LOCUS       DASSGQ010000001         6378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_607701,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000001 DASSGQ010000000
VERSION     DASSGQ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607701"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VFI55_00005"
     CDS             complement(<1..431)
                     /locus_tag="VFI55_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072452.1"
                     /translation="MSAVAGIPILSQLVAWPTEHLTEAADHWENIGGRSYGVANQVWR
                     DALSIDWHGQGAEALRTATHADMLTTSAVADQLQAASKVARTGASDLYASRSRVRYAV
                     EDARMAGFNVAEDLSVNDRSRGGSPAQRAARQAQAQTVAAD"
     gene            complement(434..733)
                     /locus_tag="VFI55_00010"
     CDS             complement(434..733)
                     /locus_tag="VFI55_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072453.1"
                     /translation="MSQLLRVDTAALQAMATRWGVSVGELSATPAPARLGLSCQASAV
                     AVNAALADVTAFTAALATRVGGHATHVCEADTGYLANEANAANAMASVIPPVTGV"
     gene            908..1663
                     /locus_tag="VFI55_00015"
     CDS             908..1663
                     /locus_tag="VFI55_00015"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HET7072454.1"
                     /translation="MLIGSHVRNDDPLAAAQADGADVVQFFLSNPQSWKKPKPRDDAA
                     TLKASALPLYVHAPYLINVASANNRVRIPSRKILQETCDAAAEINATAVIVHGGHADD
                     NDMEAGFERWVKALDYLETDVQVLLENTAGGDHAMARYFDTIGRLWDHIGDKGIGFCL
                     DTCHAWAAGEALIDAVDRIRALTGRIDLIHCNDSRDAAGSGADRHANFGTGQIDPELL
                     VAAVKAADAPVICETADEGRKDDIAFLREKTGS"
     gene            1727..3355
                     /locus_tag="VFI55_00020"
     CDS             1727..3355
                     /locus_tag="VFI55_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7072455.1"
                     /translation="MSDTHAVTNQVPPLENYNPATSAVLVEALIREGGQWGLEEVNEV
                     GALSASREAQRWGELADRHRPVLHTHDQYGYRVDEVEYDPAYHELMRAAIAHGMHAAP
                     WADERPGAHVVRAAKTSAWTVEPGHVCPISMTYAVVPALRYNPELATVYEPLLTSREY
                     DPQLRLATTKPGITAGMSMTEKQGGSDVRAGTTQATPNADGTYSLTGHKWFTSAPMCD
                     VFLVLAQAPGGLSCFLLPRVLPDGTRNRMFLQRLKDKLGNHANASSEVEYDGAIAWPV
                     GEEGRGVPTIIEMVNLTRLDCTLGSATSMRTGLTRAIHHAQHRKAFGAYLIDQPLMRN
                     VLADLAVEAEAATMVAMRMAGATDKAIRGDQTEALLRRIGLAASKYWVCKRATPHAAE
                     ALECLGGNGYVEESGMPRLYREAPLMGIWEGSGNVSALDTLRAMATRPESVEVLFAEL
                     DETAGQDPRLSAHVERLRQDLTDLDTIQYRARKVAEDICLALQGSLLVRHGHPAVAEA
                     FLTTRLDRQWGGAFGTMPAGLDLAPILERALVKG"
     gene            3418..4365
                     /locus_tag="VFI55_00025"
     CDS             3418..4365
                     /locus_tag="VFI55_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HET7072456.1"
                     /translation="MTHAIRPVDFDNLKTMTYDVTDRVARITFNRPDKGNAIVADTPL
                     ELSALVERADLDPDVHVILVSGRGDGFCAGFDLSAYADGTGSAGDRDLKGAYQGTVLD
                     GKIQAINHMSDRPWDPMIDYQMMSRFVRGFSSLMHADKPTVVKIHGYCVAGGTDIALH
                     ADQVIAAADAKIGYPPTRVWGVPAAGLWAHRLGDQRAKRLLFTGDCLTGAQAAEWGWA
                     VEAPDPKDLDERTERLVQRIAAMPVNQLIMVKLALNSALLQQGVATSRMVSTVFDGVA
                     RHTPEGHAFVADAVEHGFRDAVRHRDGPFGDYGRRASGV"
     gene            4368..5117
                     /locus_tag="VFI55_00030"
     CDS             4368..5117
                     /locus_tag="VFI55_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HET7072457.1"
                     /translation="MPNMTARSVVLSVLLGAHPAWASAGELIRLTADFGIKETTLRVA
                     LTRLVGAGDLIRSEDGYRLSDRLLARQRRQDDAMRPRTRAWRGDWVVLIVTSVGTDAR
                     TRAALRTTMHDKRFGELREGVWMRPDNLDLDLNPDVTARVRVLKAHDDAPAQLAGELW
                     DLPAWAKTGHRLLDEMAQKSRASDIPGRFVVAAAMVRHLLTDPMLPDELLPADWPGSR
                     LRAAYHDFATELAQRRDLAEQQAKTRLVEAT"
     gene            5114..5881
                     /locus_tag="VFI55_00035"
     CDS             5114..5881
                     /locus_tag="VFI55_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HET7072458.1"
                     /translation="MTDPVRVERNGPVTTVILNRPAARNAVNGPTAAALYAAFEEFDR
                     DDTASVAVLWGDGGTFCAGADLKAFGTPDANAVHRTGPGPMGPTRMVLSKPVIAAVSG
                     YAVAGGLELAVWCDLRVAEEDTVFGVFCRRWGVPLIDGGTVRLPRLIGHGRAMDLILT
                     GRAVKADEALAIGLANRVVPTGQARRAAEELAAQLAALPQQCMRSDRLSALRQWGSPE
                     PEAIDFEFASISRVAAEAAAGAGRFAAGAGRHGAAEN"
     gene            complement(5895..6371)
                     /locus_tag="VFI55_00040"
     CDS             complement(5895..6371)
                     /locus_tag="VFI55_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015292370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3060 domain-containing protein"
                     /protein_id="HET7072459.1"
                     /translation="MPDHFTDHSSRLAALVLVPAAAALVLAACSSTANPPASSSTTRA
                     SSTTTSRAAAPTSTSSGNTTASVEIGNTLNYGSLGTTATLDCANGKSLNVGGSDNTLT
                     VTGTCETVTIGGANNKITVDKVDTRISVVGLNNTVTYKDGDPKVDNLGSGNTVNKG"
BASE COUNT         1056 a         2186 c         2181 g          955 t
ORIGIN      
        1 atgtcggctg cgacggtttg tgcctgggct tgccgggcgg cgcgctgcgc gggcgagccg
       61 ccgcgggagc ggtcgttgac cgagaggtct tccgccacgt tgaagcccgc catgcgggcg
      121 tcctcgaccg cgtagcgcac ccgcgagcgg gacgcataca gatccgacgc gccggtgcgg
      181 gcaactttgg acgccgcctg cagctgatct gcgaccgcgc tggtggtgag catgtcggcg
      241 tgcgtcgcgg tacgcagcgc ctcagcgccc tggccgtgcc aatcgatcga cagcgcatct
      301 cgccacacct ggttggccac cccgtagctg cgcccaccga tgttctccca gtgatcggcc
      361 gcctcggtga gatgttcggt gggccaggcc accagctgcg aaaggatggg aatccctgca
      421 accgcagaca tcgctacacg ccggtcaccg gagggatcac gctggccatc gcgttggccg
      481 cgttagcttc attggccagg tagccggtgt cggcttcgca aacgtgggtg gcgtggccgc
      541 ctacccgagt ggccagcgcc gctgtgaagg ccgtgacgtc ggcgagggcg gcgttcaccg
      601 cgactgcgct agcctggcag gacaagccga gccgagccgg agctggcgtc gcgcttagtt
      661 cgcccaccga aacgccccac cgggtggcca tcgcctgcag ggcggcggta tccacgcgca
      721 gtagctgcga catgggcgga agttcaggtg gggccgttgg gcagcgaaaa tcagtcagcg
      781 ccgcccggaa gccaccactc gatccgcgaa tctgctagcg cctgtgctag cgctctcgaa
      841 agtcgcccat tgattcgggg cgcgacgccg ccgccaaccg tcgcacctca tcactagact
      901 ggcgaccgtg ctcattggtt cgcacgtacg caacgatgat cccttggccg cggcacaggc
      961 agacggcgcc gacgtggtgc aattcttcct cagcaacccg cagagctgga aaaagccgaa
     1021 accgcgtgac gacgccgcga cgctgaaggc gtcggccctg ccgctgtacg ttcacgcgcc
     1081 ttacctgatc aacgttgcgt ctgccaataa ccgcgttcgc atcccgtcgc gcaagattct
     1141 gcaggaaacc tgcgacgcgg ccgcggaaat caatgcgaca gcggtgatcg tgcatggcgg
     1201 tcacgccgac gacaacgaca tggaggccgg cttcgagcgg tgggtcaagg cgctcgacta
     1261 cctcgaaacc gatgtacagg tgcttttgga gaacaccgcg ggcggcgacc atgcgatggc
     1321 ccgctacttc gacaccatcg gcaggctctg ggatcacatc ggcgacaagg ggattggttt
     1381 ctgcctggac acctgccatg cgtgggccgc gggcgaggcg ctgatcgacg cagtcgaccg
     1441 aatcagagca ctgaccggcc gcatcgacct catccactgc aacgactcga gggacgcggc
     1501 cggctcgggt gcagaccggc acgccaactt cggcaccggg cagatcgatc ccgaactcct
     1561 agtcgcggca gtcaaggccg ccgacgcacc ggtgatctgc gagaccgccg acgaaggccg
     1621 taaggacgac atcgcgttcc tgcgagaaaa gaccggtagc tgaggggcac ccagtgccag
     1681 ccattacacc tcttgtgcgg ctgttgaaaa aatgtaacat ggtggcatgt cagataccca
     1741 tgccgtcacc aaccaggttc cgcccctgga gaattacaac ccggcgacct ccgcggttct
     1801 tgtcgaagcc ctgatccgcg agggcgggca gtggggcctg gaggaggtga acgaggttgg
     1861 ggcactatcc gccagccgcg aagcgcagcg atggggtgaa ctcgccgacc gccaccggcc
     1921 cgtgctgcac acccacgacc agtacgggta ccgcgttgac gaggtggagt acgacccggc
     1981 ctaccacgag ctgatgcgtg cggcgatcgc ccatggcatg cacgccgcgc cgtgggccga
     2041 cgagcggccg ggcgcccacg tggtgcgggc cgccaagacc tcggcgtgga cggtggagcc
     2101 gggacacgtc tgcccgatct cgatgaccta cgccgtcgtc cccgcgctgc gctacaaccc
     2161 cgagctggcg acggtctacg agccgttgct gaccagccgc gagtacgacc cgcagctcag
     2221 gctggccacc accaagcccg gcatcaccgc cggcatgtcg atgaccgaaa agcagggcgg
     2281 ctccgatgtg cgcgccggca ccacccaggc caccccgaac gccgacggca cctactccct
     2341 gaccggtcac aagtggttca cctcggcgcc gatgtgcgac gtcttcctgg tgctcgcgca
     2401 ggcgccgggc gggctgtcgt gcttcctgct gccgcgggta ctgcctgacg gcacccgcaa
     2461 ccggatgttc ctgcagcggc tcaaggacaa gctcggcaac cacgccaacg cctccagcga
     2521 ggtcgagtac gacggcgcca tcgcctggcc ggtgggcgag gagggccgcg gcgtcccgac
     2581 catcatcgag atggtcaacc tgacccggct ggactgcacg ctgggcagcg ccaccagtat
     2641 gcgcaccggc ctgacccgcg ccatccacca cgcccagcac cgaaaggcgt tcggcgctta
     2701 cctgatcgac cagccgctga tgcgtaacgt gctggccgac ctggccgtcg aggccgaggc
     2761 cgcgacgatg gtcgcgatgc ggatggcggg cgccaccgac aaggcgatac gcggagacca
     2821 aacagaggcg ctgctgcgcc gcatcggcct ggcggccagc aagtactggg tgtgcaagcg
     2881 ggccactccg cacgccgccg aagccctgga atgcctgggc ggcaacggct acgtcgaaga
     2941 atccgggatg ccccggctct accgtgaggc gccgctgatg ggcatctggg aaggctcggg
     3001 caacgtcagc gcactggata cactgcgcgc catggctacc cgccccgaaa gcgtcgaggt
     3061 gctgttcgcg gagctcgacg aaaccgcggg ccaggatccg cgcctaagcg cccacgtcga
     3121 acggctgcgg caagacctca ccgacctgga caccatccaa taccgcgccc gcaaggtcgc
     3181 cgaggacatt tgcctggcgc tgcagggctc gctgctggtg cgccacggcc accccgccgt
     3241 cgctgaggcg ttcctgacca cccggctcga ccgccagtgg ggcggggcgt tcggcaccat
     3301 gccggccggg ctggacctgg cgcccatcct cgagcgtgcc ctggtaaaag gctgagcggc
     3361 gtcgatgacc cacgccgtcg acgatgcaga gcgaagcgat gaggaggagc ggagtcaatg
     3421 acccacgcga tcaggccggt cgacttcgac aacctgaaaa ccatgacgta cgacgtcacc
     3481 gaccgcgtcg cgcggatcac cttcaaccgg ccggacaagg gcaacgcgat cgtcgccgac
     3541 accccgctgg agctctccgc cctggtggag cgagccgacc tggaccccga cgtgcacgtc
     3601 attctggtgt ccggtcgcgg cgacgggttc tgcgcgggct ttgacctgtc cgcctacgcc
     3661 gacggcaccg gctcggccgg cgaccgggac cttaaaggcg cctaccaggg caccgtgctg
     3721 gacggcaaga tccaggccat caaccatatg tcagaccggc catgggaccc gatgatcgac
     3781 taccagatga tgagccgctt cgtgcgcggg ttctccagcc tgatgcacgc tgacaagccg
     3841 accgtggtca agatccacgg ctactgcgtg gccggcggca ccgacatcgc gctgcatgcc
     3901 gaccaggtga tcgccgcagc cgatgcgaag atcggctacc cgcccacgcg ggtgtggggc
     3961 gtcccggcgg ccggcctgtg ggcgcatcgc ctcggtgatc aacgcgccaa acgcctgctg
     4021 ttcaccggcg attgcctcac cggtgcgcag gccgccgagt ggggatgggc ggtcgaggcg
     4081 ccggacccca aggacctcga cgagcgcacc gagcgactcg tccaacggat cgccgcaatg
     4141 ccggtcaacc agctgatcat ggtcaaactc gcactcaatt ccgccctgct gcaacagggt
     4201 gtggccacca gcaggatggt cagcacggtg ttcgacggcg tcgcccggca cacgcccgag
     4261 gggcacgcct tcgtcgccga tgcggtcgag cacggattcc gggatgcggt acggcatcgc
     4321 gacgggccgt tcggcgacta tggccgtcgg gcatccgggg tctaggcatg ccgaacatga
     4381 ccgcccggtc cgtggtgctc agcgtgctgc tcggtgctca tcccgcgtgg gccagcgccg
     4441 gcgaattgat caggctgacc gctgatttcg gtatcaagga gacgacattg cgggtcgcgc
     4501 tgacgcgctt ggtcggcgcc ggcgacctga tccggtccga ggacggctac cggctttcgg
     4561 atcgcttgct ggcccgccag cgccggcagg acgatgccat gcgcccccgg acccgggcct
     4621 ggcgcggaga ctgggtggtg ctgatcgtca ccagcgtggg caccgatgcc cgcacccggg
     4681 ccgcactgcg aactaccatg cacgacaagc gcttcggtga attgcgcgaa ggggtatgga
     4741 tgcggcccga caacctggac ctggacctga atcccgacgt gacggcccgg gtgcgggtcc
     4801 tcaaggcaca cgacgacgcg cccgctcagc tggccggcga gctatgggac ctgcccgcct
     4861 gggcaaaaac cggccaccgg ctgctcgacg agatggccca gaagagtcgg gcgtctgaca
     4921 ttcccggccg gtttgtggtg gccgcggcga tggtgcgcca cctgctcacc gacccgatgc
     4981 tgcccgacga actgctgccc gccgattggc cgggctcgcg gctgcgtgcc gcctaccacg
     5041 acttcgccac cgaactggcg cagcgacgtg acttggcgga acaacaagcg aagacacgac
     5101 tcgtggaggc gacatgactg atccggtgcg ggtagagcgc aacggcccgg tgaccacggt
     5161 gatcctcaac cggcccgcgg cccgcaacgc cgtcaacggc ccgaccgccg cggcgctgta
     5221 cgccgcgttc gaagaattcg accgcgacga caccgcctcg gtggccgtgc tgtggggcga
     5281 cggcggaacc ttttgcgccg gagccgattt gaaggccttc ggcacacccg acgccaatgc
     5341 cgtgcaccgc accggcccgg gcccgatggg gccgacccgg atggtgctgt ccaaaccggt
     5401 gatcgctgcc gtcagcggat acgccgtcgc cgggggcctg gagttggccg tctggtgcga
     5461 tctgcgggtc gccgaggagg acaccgtgtt cggcgtgttc tgccgccgct ggggggtgcc
     5521 gctcatcgac ggcggcaccg tgcggctgcc ccggctgatc gggcacggcc gcgcgatgga
     5581 cctgatcctc accggccgcg ccgtgaaagc cgatgaagcg ctcgcgatcg ggctggccaa
     5641 ccgggtcgtg cccaccgggc aggcccgccg ggccgccgag gagctcgccg ctcagctcgc
     5701 cgcgctgccg cagcagtgca tgcgatcgga tcggctgtcg gcattgcggc agtgggggag
     5761 cccggaaccg gaggccatcg acttcgaatt cgccagcatt tcccgggtcg ccgccgaggc
     5821 cgcggcgggg gcggggcggt tcgccgcggg tgcgggccgc cacggcgcgg ccgagaacta
     5881 gaccgcgccg gcgctcagcc cttgttgacc gtgttgcccg agccgaggtt gtcgaccttg
     5941 gggtcgccgt ccttgtaggt gacggtgttg ttgagtccca ccacgctgat gcgggtgtcg
     6001 accttgtcga cggtgatctt gttgttggca ccgccgatgg tcacggtctc gcaagtaccg
     6061 gtgacggtca gcgtgttgtc cgagccgccc acgttcagcg acttgccgtt ggcgcagtcg
     6121 agggtggcgg tggtccccag cgacccgtaa ttcagcgtgt tcccgatctc gaccgaagcg
     6181 gtggtgttcc cggagctcgt gctggtcggc gcggccgcgc ggctcgtggt tgtgctgctg
     6241 gccctcgtcg tcgacgagct cgcgggcggg ttggccgtcg aactgcacgc tgccagcacc
     6301 aacgccgcgg cggccggtac cagaaccagc gcggccagcc tggacgagtg gtcggtgaag
     6361 tggtcaggca ttgatcct
//
LOCUS       DASSGQ010000002         4269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_486222,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000002 DASSGQ010000000
VERSION     DASSGQ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486222"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1778)
                     /locus_tag="VFI55_00045"
     CDS             complement(<1..1778)
                     /locus_tag="VFI55_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET7072460.1"
                     /translation="MTAANEHRYDTDILDTLAQRVMVGDGAMGTQLQAADLTLDDFRG
                     LEGCNEILNETRPDVLETIHRAYFEAGADAVETNTFGCNLSNLGDYDIADKIRDLSQR
                     GTAVARRVADELSTPDRRRYVLGSMGPGTKLPTLGHTEYHVIRDAYALAAVGMLDGGA
                     DAILVETCQDLLQLKAAVLGSRRAMAQAGRHIPVFAHVTVETTGTMLLGSEIGAALTA
                     VEPLGVDMIGLNCATGPAEMSEHLRYLSRHARIPVSVMPNAGLPVLGPNGAEYPLLPD
                     ELAEALAGFLTEFGLSLVGGCCGTTPEHIRQVADAVADFNDGTARRGERHVTYEPSVS
                     SLYSAVPFAQDASVLVIGERTNANGSKGFREAMIAEDYQKCLDIAKDQTRDGAHLLDL
                     CVDYVGRDGVADMTALASRLATASTLPIMLDSTETPVLRAGLEHLGGRCTINSVNYED
                     GDGPESRFHKTMELVAEHGAAVVALTIDEEGQARTVEKKVEIAERLINDITGNWGVDE
                     SSILIDTLTFTIATGQEESRKDGIETIGAIRELKKRHPDVQTTLGLSNISFGLNPAAR
                     QVVNSVFLHECQEAGLDSAIVHASKIL"
     gene            1906..2781
                     /locus_tag="VFI55_00050"
     CDS             1906..2781
                     /locus_tag="VFI55_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HET7072461.1"
                     /translation="MTPPDGTPLPEMHNTIVVAAFEGWNDAGDAASDALEHLDAIWEA
                     DPIVEIDDEAYYDYQVNRPVIRQVDGVTRELVWPAMRITHCRPPGSDRDVVLMHGVEP
                     NMRWRTFCAELVAIADKLNVDTVVILGALLADTPHTRPVPVSGAAYSPESAKRFGLEE
                     TRYEGPTGIAGVFQDACVAAGIPAVTFWAAVPHYVSTPPNPKATVALLRRVEDVLDIE
                     VPLADLPAQAEEWEQAITEMAAEDEDLAEYVLSLEQRGDAEVDVNDALGKIDGDALAA
                     EFERYLRRRRPGFGR"
     gene            complement(2778..3653)
                     /locus_tag="VFI55_00055"
     CDS             complement(2778..3653)
                     /locus_tag="VFI55_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7072462.1"
                     /translation="MTSLQGKVVFITGGARGIGAEVARRLRNKGAKLVLTDLDKAERT
                     ALAAELGEERVLTVVADVRDLPAMQAAADEAVQHFGGIDVVVANAGIAGYGSVLQVDP
                     EAFKRVLDVNLLGVFHTVRATLPALIDRRGYLLIVSSLAAFAASPGMASYDMTKAGNE
                     HLANALRFEVGHTGVNVGVAHMSWIDTALVRDTEADLPSFGRLLAALPWPLNKTTSVT
                     RCAEAFVKGIEDRRDRVYCPRWVILFRWLKPVLSTRVGEFPARKAAAELPQLDAEVAA
                     LGRSSSAYNERRENS"
     gene            complement(3690..>4269)
                     /locus_tag="VFI55_00060"
     CDS             complement(3690..>4269)
                     /locus_tag="VFI55_00060"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103677.1"
                     /note="mycothiol biosynthesis; catalyzes the ligation of
                     cysteine to a mycothiol precursor; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine--1-D-myo-inosityl
                     2-amino-2-deoxy-alpha-D-glucopyranoside ligase"
                     /protein_id="HET7072463.1"
                     /translation="GAGRPGWHIECAAIALSRIRSGLDIQGGGSDLTFPHHEFTAAHA
                     ECVRGERRFARHYVHTGMIGWDGHKMSKSRGNLVLVSTLRAKGVEPAAIRLGLLAGHY
                     RADRFWSDEVLDEAVARLQRWRIATALPDGPDATDLIARVRQYLADDLDSPKAIAALD
                     GWTTDALEYGGHDGAAPSLVATAIDALLGVDL"
BASE COUNT          679 a         1441 c         1449 g          700 t
ORIGIN      
        1 aggatcttgg acgcgtgcac gatcgccgag tccagaccgg cttcctgaca ttcgtgcagg
       61 aacacggagt tgactacttg acgcgcggcg ggattgaggc cgaaggagat gttggacaac
      121 ccgagcgtgg tctgcacgtc ggggtggcgt ttcttcagct cccggatcgc cccgatggtc
      181 tcgatgccgt ctttgcgcga ctcctcctgg ccggtggcga tggtgaaggt caacgtatcg
      241 atgaggatgg acgattcgtc gacgccccaa ttgccggtga tgtcattgat caaccgctcg
      301 gcgatctcga ccttcttctc gacggtgcgg gcctggccct cctcgtcaat ggtcagcgcg
      361 accaccgccg cgccgtgctc ggcgaccagc tccatggtct tgtggaaacg cgactccggg
      421 ccgtcgccgt cctcgtagtt gaccgagttg atcgtgcagc ggccgcccag gtgctccaaa
      481 cccgctcgca gcaccggggt ttcggtggag tccagcatga tcggcaacgt cgacgccgtc
      541 gccagccggc tggccagcgc cgtcatgtcg gccacgccgt cgcgacccac gtagtcgacg
      601 cacaggtcca gcaggtgcgc gccgtcgcgg gtctggtcct tggcgatgtc caggcacttc
      661 tggtagtcct cggcgatcat cgcctcgcgg aaacccttgg agccgttggc gttcgtgcgc
      721 tccccgatca ccagcaccga ggcgtcctgc gcgaacggca cggcgctgta cagcgacgac
      781 accgacggct cgtaggtcac gtgacgctcg ccgcgccgtg cggtcccgtc gttgaagtcg
      841 gcgacggcat cggccacctg gcggatgtgt tcgggggtgg tgccgcagca gccaccgacc
      901 agcgacaggc cgaactcggt gaggaatccg gccagcgcct cggccaattc gtcgggcagc
      961 aacgggtatt cggcgccgtt ggggcccagc accggcagcc cggcgttggg catcaccgac
     1021 accgggatgc gggcatgcct ggacaggtag cgcaggtgct cgctcatttc ggcggggccc
     1081 gtcgcacagt tcagcccgat catgtccaca ccgagcggct cgacggccgt cagcgccgcg
     1141 ccgatctcgc tgcccagcag catggtgccg gtcgtctcca cggtgacgtg ggcaaaaacc
     1201 ggaatgtgcc gtccggcctg ggccatcgcg cggcgcgatc ccagcaccgc ggccttcagc
     1261 tgcagcaggt cctggcaggt ttccaccagg atggcgtcgg cgccgccatc gagcatgccc
     1321 accgcagcca aggcgtacgc gtcgcggatg acgtggtact cggtgtggcc cagggtcggc
     1381 agcttggtgc ccggccccat cgaccccagc acgtaccgcc tgcggtccgg ggtgctcagc
     1441 tcgtcggcga cccggcgcgc gaccgcggtg ccgcgctgcg agaggtccct gatcttgtcg
     1501 gcgatgtcgt agtcgccgag gttggacagg ttgcagccga aggtgttggt ctcgacggcg
     1561 tccgcgccgg cctcgaagta ggcgcggtgg attgtctcca gcacgtcggg gcgcgtctcg
     1621 ttaaggatct cgttgcagcc ttccaggccg cggaagtcgt cgagcgtgag atcggccgcc
     1681 tgcagctggg tgcccatcgc accgtcgccg accatcacac gctgcgccaa agtgtccagg
     1741 atatcggtgt cgtagcggtg ctcattagcg gcagtcacgc cacaaggata gtcggcctat
     1801 gcatagaggc gagtcgtcga cggctccgcc ggattacccg cctcgccccc tcagcggccc
     1861 ggatggcccg catcgtcggc aggaagaagg ccgtaccctg attctgtgac cccgccggat
     1921 ggcaccccgc tgcccgaaat gcacaacacc atcgtcgttg cggcgttcga gggctggaac
     1981 gacgcgggcg acgcggccag cgatgcgctc gagcacctcg acgccatctg ggaagcggat
     2041 ccgatcgtgg agatcgacga cgaggcgtat tacgactacc aggtgaatcg cccggtcatc
     2101 cgccaggtcg acggcgttac ccgggagctg gtgtggccgg cgatgcgaat cacccattgc
     2161 cgcccacccg gcagcgaccg ggacgtcgtg ttgatgcatg gcgtggagcc caacatgcgc
     2221 tggcgcacat tctgtgccga gctggtggcc atcgcggaca agctcaacgt cgataccgtc
     2281 gtgatcctcg gggccctgct ggccgacacc ccgcacaccc ggccggtgcc ggtgtcgggg
     2341 gcggcctact cccccgagtc ggcgaaacgc ttcggcctcg aggaaacgcg gtacgagggc
     2401 cccaccggga tcgccggggt gttccaggac gcctgcgtgg ccgcggggat cccggcggtg
     2461 acgttctggg cggcggtgcc gcactacgtg tccaccccgc cgaacccgaa ggcgacggtg
     2521 gcgctgctgc gccgcgtcga agacgtgctc gacatcgagg tcccgctggc cgacctgccg
     2581 gcgcaggccg aggagtggga gcaggcgatc accgagatgg ccgccgagga tgaggacctg
     2641 gccgaatacg tgttgtcgct ggagcagcgc ggcgacgccg aggtcgatgt gaacgacgcg
     2701 ctgggcaaga tcgacggcga cgcgctggcc gcggagttcg aacgttatct gcgccgccgc
     2761 cgcccggggt tcgggcgcta ggaattctcc cggcgctcgt tgtacgcgct gctggaacga
     2821 ccgagcgcgg cgacttcggc gtccaattgc ggcagctcag cagcggcctt gcgggccgga
     2881 aattcgccga cccgggtgga caacaccggc ttgagccaac ggaacaggat tacccagcgc
     2941 gggcagtaca cgcggtccct gcggtcctcg atccccttga caaatgcctc agcacacctg
     3001 gttaccgagg tggttttgtt caatggccaa ggcagcgcgg caagcagtcg accgaacgac
     3061 ggcaggtcgg cctcggtgtc gcgaaccagc gcggtgtcga tccaggacat gtgcgccacg
     3121 ccgacgttca ccccggtgtg gccgacctcg aatcgcagcg cgttggccag atgctcgttc
     3181 ccggctttcg tcatgtcgta cgacgccatc cctggagacg ctgcgaatgc ggccagcgac
     3241 gagacgatca acaggtagcc gcggcggtcg atcagcgccg gcaaggtggc gcgcacggtg
     3301 tgaaagacgc cgagcaggtt gacgtccagc acccgcttga acgcctccgg gtcgacctgc
     3361 agcaccgaac cgtagccggc gatcccggcg ttggccacga cgacgtcgat gccgccgaaa
     3421 tgttggacgg cctcgtcggc cgcggcctgc atggcgggca gatcgcgcac gtcggctacc
     3481 acggtcaaca cccgttcttc gccgagttca gcggccagcg cggtccgctc ggctttgtcg
     3541 agatcggtca gcaccagttt ggcgcccttg ttgcgcagcc gacgggctac ctcggctccg
     3601 atcccccggg cgccaccggt gatgaagaca actttgccct gcagcgacgt catggccgaa
     3661 aacgtacctc gccggtgact ctgctgcgat tagaggtcca ccccgagtaa cgcgtcaatc
     3721 gcggtcgcca ccagactcgg cgccgcgccg tcatggccgc cgtattccag tgcatcggtg
     3781 gtccaaccat caagtgctgc aatggctttg ggggaatcaa gatcgtcggc caaatactgc
     3841 cgcacccggg cgatcaggtc ggtcgcgtcg ggtccgtcgg gcagcgcggt cgcgatgcgc
     3901 cagcgctgca gccgggcgac cgcctcgtcg agcacttcgt cactccagaa ccgatcggcg
     3961 cgatagtggc cggccagcag gcccagccgg atggccgccg gctcaacgcc tttggcgcgc
     4021 aacgttgaga ccagcaccag attgccgcgg ctcttggaca tcttgtgccc gtcccagccg
     4081 atcatcccgg tatgcacgta gtgccgagcg aatcgccgct cgccccggac acattcggcg
     4141 tgcgcggcgg tgaattcgtg atggggaaag gtcaggtcgc tgccgccgcc ctggatgtcc
     4201 aggccgctgc gaatgcggct gagcgcgatc gccgcgcatt cgatgtgcca gccgggtcgc
     4261 ccggcaccg
//
LOCUS       DASSGQ010000003        14820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1306510,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000003 DASSGQ010000000
VERSION     DASSGQ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14820
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306510"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="VFI55_00065"
     CDS             complement(<1..179)
                     /locus_tag="VFI55_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 family protein"
                     /protein_id="HET7072464.1"
                     /translation="MPTDYDAPRRTETDDVSEDSLEELKARRNDSSSAVVDVDESESA
                     ESFELPGADLSGEELS"
     gene            351..1001
                     /gene="cei"
                     /locus_tag="VFI55_00070"
     CDS             351..1001
                     /gene="cei"
                     /locus_tag="VFI55_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope integrity protein Cei"
                     /protein_id="HET7072465.1"
                     /translation="MVAQITEGTAFDKHGRPFRRRNPRPAIFVLAFLVVVAGVVWTVA
                     LTRPAKVHEAVVCNPPPQQEGSAPAQLGEQVSRAAMMDVTPAKLSDTKVRVLNASGRG
                     GQAGDVAGAMKDLGFAQPTAANDPIYAGSRLNCQGQIRFGTAGQATAAAVWVVAPCTE
                     LFNDNRADDSVDLALGTDFTALAHNDDIDSVLASLRPGATEGADPVLLQKIHASSC"
     gene            complement(1033..1917)
                     /locus_tag="VFI55_00075"
     CDS             complement(1033..1917)
                     /locus_tag="VFI55_00075"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET7072466.1"
                     /translation="MTRPDDEPARLRSVAEMLAAEAAEFVRRRRTEVFGTESGASTAP
                     ASDVVRAKTTPTDPVTVVDTETERLLRDRLAQLRPGDPILGEEGGGPTDPTATPADAV
                     TWVLDPIDGTVNFVYGIPAYAVSVGAQVNGVSVAGAVADVVAGRVYSAAIGLGAHVAD
                     EHGTGPLRCATVEDLSMALLGTGFGYSRRRRATQAALLARMLPVVRDVRRIGSAALDL
                     CMVAAGRLDAYYEHGLQVWDCAAGALIAAEAGARVMLPAPDVPAGSAGLVVAAAPGIA
                     DELLAALDRVDGLDPILD"
     gene            2019..2828
                     /locus_tag="VFI55_00080"
     CDS             2019..2828
                     /locus_tag="VFI55_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET7072467.1"
                     /translation="MTTTDSTTDTPSTDAPTGTPQRRGFGIDVGGSGIKGGIVDLDTG
                     LLIGDRIKLLTPQPATPSAVAKTIAEVVNGFGWTGPLGVTYPGVVTHGVVQTAANVDK
                     AWIGTNAGDVIGAELNGQAVTILNDADAAGLAEARYGAGRDQSGVVVLLTFGTGIGSA
                     VIHNGTLLPNTEFGHLEVGGKEAEQRAASSVKDKNDWSYEKWAKQVTKVLVAIENALW
                     PDLFIAGGGISRKADQWLPLLENRTRVVAADLLNTAGIVGAAMASTSDVTH"
     gene            3006..4580
                     /locus_tag="VFI55_00085"
     CDS             3006..4580
                     /locus_tag="VFI55_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7072468.1"
                     /translation="MAAIKASPATDEPVKRTATKSPSSPAKRSAAKAANGSTPAKRTA
                     KSASPAAKPAPGHEATAPDELSEATKKTRASAKAGGAKASSARGTKAADNDAPDDPDA
                     SLQPLVADAAVEELDAEPDLEDEPGVDIDIDADELVLDDLEDDVAADGDEADLDPADV
                     EEAEAAEAPLAVKAGKAATAEDEEIAEPTEKDKASGDFVWDEDESEALRQARKDAELT
                     ASADSVRAYLKQIGKVALLNAEEEVELAKRIEAGLYATQLMSEHAERGDKLPAAQRRD
                     MMWICRDGDRAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYT
                     KGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQRELLQDLGREPTP
                     EELAKEMDITPEKVLEIQQYAREPISLDQTIGDEGDSQLGDFIEDSEAVVAVDAVSFT
                     LLQDQLQSVLETLSEREAGVVRLRFGLTDGQPRTLDEIGQVYGVTRERIRQIESKTMS
                     KLRHPSRSQVLRDYLD"
     gene            4690..5136
                     /locus_tag="VFI55_00090"
     CDS             4690..5136
                     /locus_tag="VFI55_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020724812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET7072469.1"
                     /translation="MPANRKLVSSESDFESTVGYSRAVRVGPHVAVAGTTGAGPAGDI
                     SAQTRDALRRIEIALQQAGAALTDVVRTRIYVTDISRWREVGAAHAQVFGAIRPAASM
                     VEVSALIEPDLLVEIEADAYVESEGGSPAAFDVRGTGGNVPSAAGG"
     gene            complement(5052..5465)
                     /locus_tag="VFI55_00095"
     CDS             complement(5052..5465)
                     /locus_tag="VFI55_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HET7072470.1"
                     /translation="MNIAADVLVHLCGAEEWSRARSRGEIRPPNAPASGSNATAFIHL
                     STPEQVHLPANRLYRGRGDLVLLHINPALLDSPVRWEPGVATDPESMLFPHLYGPLPV
                     RAVLNVTAYPPAADGTFPPVPRTSNAAGDPPSDST"
     gene            5599..5784
                     /locus_tag="VFI55_00100"
     CDS             5599..5784
                     /locus_tag="VFI55_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072471.1"
                     /translation="MNATLTSPELTRADRCDRCGAAARVRAKLPSGLELLFCQHHANQ
                     HEAKLAELAAVIEVSAS"
     gene            5847..6836
                     /locus_tag="VFI55_00105"
     CDS             5847..6836
                     /locus_tag="VFI55_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET7072472.1"
                     /translation="MNAQVAKPTRHHIWRISLRTLSKSWDDSIFSEAAQAGFWSALSL
                     PPLLLGMLGSLTYVAPLFGPDTLPEIEKTIVSTAHKFFSPSVVNEIIEPTIRDITANA
                     RGEVVSLGFLISMWAGSSAISSYVDAVVAAHDQTPLRHPVRQRFFALFLYMVMQVFVV
                     AIAPIIVLGPRKLREHIPAGLANMLHYGYYPALVLGLMIGVIVLYRVSLPVPLPTHRL
                     IFGAVLATAVFLIATLGLRIYLGWITSTGYTYGALSTPIAFLLYAFFAGFALMLGAEL
                     NAAIQEEFAAPATHAHRLRSWVISRVPALQRTVDSPTEPALPRRDVATAEPPT"
     gene            complement(6837..7076)
                     /locus_tag="VFI55_00110"
     CDS             complement(6837..7076)
                     /locus_tag="VFI55_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HET7072473.1"
                     /translation="MQTQTIERTETDERVDDGTGSDTPKYFHYVKKDKIAESAVMGSH
                     VVALCGEVFPVTRAAKPGSPVCPDCKRIYEQLKKG"
     gene            7153..7563
                     /locus_tag="VFI55_00115"
     CDS             7153..7563
                     /locus_tag="VFI55_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3099 domain-containing protein"
                     /protein_id="HET7072474.1"
                     /translation="MKRRSHKRFDDGFDDFDDKGQPVLITAAAPSYEEEQRTRIRKYL
                     TLMAFRVPALILAAVAYGAWHNGLISLAIVAVSVPLPWMAVLIANDRPPRRPEEPRRF
                     DAAPRRTRLFPRAELPALDPPRHPEPPPDWDSRE"
     gene            7748..8707
                     /gene="sigB"
                     /locus_tag="VFI55_00120"
     CDS             7748..8707
                     /gene="sigB"
                     /locus_tag="VFI55_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor
                     SigB"
                     /protein_id="HET7072475.1"
                     /translation="MANATTSRFDGDLDAQSPAADLVRVYLNGIGKTALLNAAGEVDL
                     AKRIEAGLYADHLLETRKRLGENRKRDLEAVVRDGEAARRHLLEANLRLVVSLAKRYT
                     GRGMPLLDLIQEGNLGLIRAMEKFDYTKGFKFSTYATWWIRQAITRGMADQSRTIRLP
                     VHLVEQVNKLARIKREMHQNLGREATDEELAAESGIPIEKINDLLEHSRDPVSLDMPV
                     GSEEEAPLGDFIEDAEAMSAENAVIAELLHTDIRSVLATLDEREHQVIRLRFGLDDGQ
                     PRTLDQIGKLFGLSRERVRQIERDVMCKLRSGERADRLRSYAS"
     gene            8842..9534
                     /gene="ideR"
                     /locus_tag="VFI55_00125"
     CDS             8842..9534
                     /gene="ideR"
                     /locus_tag="VFI55_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent transcriptional regulator IdeR"
                     /protein_id="HET7072476.1"
                     /translation="MNELVDTTEMYLRTIYDLEEEGVTPLRARIAERLDQSGPTVSQT
                     VSRMERDGLLHVAGDRHLELTDKGRALAIAVMRKHRLAERLLVDVIGLPWEEVHAEAC
                     RWEHVMSEDVERRLVKVLNNPTTSPFGNPIPGLLDLGVGPESGADDAHFVRLTELPAG
                     SPVAVVVRQLTEHVQGDIDLITRLKDAGVVPNARVTVETGPAAGVTILIPGHENVTLP
                     HEMAHAVKVEKV"
     gene            complement(9618..10676)
                     /locus_tag="VFI55_00130"
     CDS             complement(9618..10676)
                     /locus_tag="VFI55_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4192 domain-containing protein"
                     /protein_id="HET7072477.1"
                     /translation="MTTHRGDCELNRPGALIAALPAVLGFVPENSLVLVSLTGGELGS
                     VLRVDLGEELVDRVGHLAEVAAAAEPEAAIAVIVAADGAHCPRCNQDYRQLCATLADA
                     LSAYDIELWAAHVVDRVARGGRWHCVDGCGSSGAVDDPSASPLAAAAVLDGRRLYPRR
                     ADLQAVIAADDGARSAELASSMRQRAGAREAAHRADPAGCSRRDVEDAMAAAGRVAGG
                     HPLSDAELAQLGCALSDVLVRDTLYALAVGEIAGEAESLWALLARTLPEPWRVEALVL
                     LAFSAYARGDGPLAGVSLEAALRCDSEHRMAGMLDTALQSGLRPEQIRELAVTGYRVA
                     KRLGVRLPPRRAFGRRAG"
     gene            10790..12196
                     /gene="sthA"
                     /locus_tag="VFI55_00135"
     CDS             10790..12196
                     /gene="sthA"
                     /locus_tag="VFI55_00135"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HET7072478.1"
                     /translation="MQEYDMVVIGSGPGGQKAAIASAKLGKSVAIIERGRMLGGVCVN
                     TGTIPSKTLREAVLYLTGMNQRELYGASYRVKDRITPADLLARTQHVIGKEVDVVRNQ
                     LMRNRIDLLIGHGRFLDPHTVVVEDPGRREMTTVSGEYIVIATGTRPARPSGVEFDED
                     RVLDSDGILDLKTLPASMVVVGAGVIGIEYASMFAALGTKVTVVEKRDDMLDFCDPEV
                     VEALKFHLRDLAVTFRFGEAVTAVDVGSAGTVTTLASGKQIPAETVMYSAGRQGQTDH
                     LDLQNAGLEVEGRGRIWVDDRFQTKVEHIYAVGDVIGFPALASTSMEQGRLAAYHAFG
                     EACEGMTDLQPIGIYSIPEISYVGATEVELTKNAVPYEVGVARYKELARGQIAGDSYG
                     MLKLLVSTEDRKLLGVHIFGTSATEMVHIGQAVMGCGGTVDYLVDAVFNFPTFSEAYK
                     VAALDVTNKMRALSQFRR"
     gene            complement(12207..13028)
                     /locus_tag="VFI55_00140"
     CDS             complement(12207..13028)
                     /locus_tag="VFI55_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET7072479.1"
                     /translation="MRVPFVLLCPVVGLATLVTSCTRPAEHASIPPVSPSRVSGPVQH
                     LSQPQQQAVAGPVDPDRRVGAIFLDGGNVHVCTGSVVHSGNGNLVMTAAHCLAGASRI
                     TFVPGFAGTAAPPDMWTADAVYLDPRWSASRDPRADYAIVRVSSTGGGSVESHVGLAL
                     TLGVAPPPGSHVTVMGYPAGVGGSPIGCQTSTSVNDSGFPSLVCEGLVGGTSGGPWVS
                     GTTLTGVIGGFERGGCAENVSYSAPFDEQTAQLLVRAEAGGPGDMVPNGPDDSCS"
     gene            13268..14236
                     /locus_tag="VFI55_00145"
     CDS             13268..14236
                     /locus_tag="VFI55_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HET7072480.1"
                     /translation="MAHDQNPDDYQPGQPGLYELEFPAPQLSTNDGRGPVLVHALEGF
                     SDAGHAIRLSATHLKASLDTELVASFAIDELLDYRSRRPLMTFKTDHFTSYEDPELSL
                     YALRDSVGTPFLLLAGMEPDLKWERFITAVRLLAERLGVRRTIGLGTVPMAVPHTRPI
                     TLTAHSNDGELIADFQPWISEIQVPGSASNLLEYRMAQHGHEVVGFTVHVPHYLTQTD
                     YPAAAQALLEQVAKTGSLELPLSALSDAAAEIRAKIDEQVQASAEVAQVVAALERQYD
                     AFIDAQENRSLLTRDEDLPSGDELGAEFERFLAQEASKKSEDDDQT"
     gene            14291..>14820
                     /locus_tag="VFI55_00150"
     CDS             14291..>14820
                     /locus_tag="VFI55_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7072481.1"
                     /translation="MTERKRKSNLRPVREVTTPRLEFRTIHGYQRAYRIAGSGPAILL
                     IHGIGDNSTTWNAVQAKLAQRFTVIAPDLLGHGKSDKPRADYSVAAYANGMRDLLSLL
                     EIERATIIGHSLGGGVAMQFAYQFPHMVERLILVASGGVTKDVNLVLRLASLPMGGEA
                     LALLRLPLVLPAIQLAG"
BASE COUNT         2663 a         5040 c         4847 g         2270 t
ORIGIN      
        1 gaaagttctt caccagacag gtcggcgccg ggcagctcga aagactcagc ggattcggat
       61 tcgtcaacgt cgaccacggc cgaggacgag tcgttccgcc gtgctttgag ttcctccagc
      121 gagtcctccg agacgtcgtc agtctcggta cgccgcggag cgtcataatc ggtaggcatc
      181 gcccaatccc ctcacatgcc ttcgttacct caagcaacgc tttgtaccag cgtcgaacgc
      241 atccaccaaa cgattcgtgc ccggatcccg ccccattgcc atgtgattta catcacacca
      301 acgacctgac ccttgtattg cgtcttaaac agtgcgttta gagtgcacct gtggtcgcac
      361 aaatcaccga gggtaccgct ttcgacaagc acggtaggcc atttcggcgg cgaaatcccc
      421 gccccgccat attcgtactc gccttcctgg tggtggtggc gggtgtggtg tggaccgtcg
      481 cgctgacgcg accggcgaag gtgcacgagg cggtcgtctg caacccgccg ccgcaacagg
      541 aggggtcggc accggcgcag ctcggtgagc aggtgtcgcg ggccgccatg atggacgtca
      601 cgcccgcgaa actcagcgac accaaggtcc gcgtcctcaa cgcgagcggc cggggtggcc
      661 aagccggcga cgtcgccggt gccatgaaag atctgggctt tgcgcagccg accgcggcca
      721 acgacccgat ctatgccggc agcaggctga actgccaggg ccagatccgc ttcggtacgg
      781 ccgggcaagc caccgccgcc gccgtgtggg tggtggcgcc gtgcaccgag ctgttcaacg
      841 acaaccgcgc cgacgactcc gtcgacctcg ctctggggac agacttcacc gcgctggccc
      901 acaacgacga catcgactcg gtgctggcca gcctgcgccc cggcgccacc gagggggcag
      961 atcccgtgct gctgcagaaa attcacgcca gcagctgctg aggcgccggc atcgggtcag
     1021 tccagaatcg ggtcagtcca gaatcgggtc gaggccgtcg acccggtcca gggccgccag
     1081 tagctcgtcg gcgattccgg gcgcggcagc caccaccaat ccggcgctgc ccgccgggac
     1141 gtccggtgcc ggcagcatca cccgggcgcc tgcctccgcc gcgatcagtg caccggcggc
     1201 gcaatcccag acctgcaatc cgtgctcgta gtaggcatcc agccggcccg ccgcgaccat
     1261 gcacaagtcc agagccgcag acccgatgcg acgcacgtcg cggaccaccg gcagcattcg
     1321 cgccaacagg gccgcctggg tggcgcggcg ccgtcgtgag taaccaaaac ccgtgcccag
     1381 caacgccatc gacagatcct cgacggttgc acaccgcaac ggtcctgttc cgtgttcgtc
     1441 ggcgacatgc gcgccgaggc cgatcgcagc cgagtagacc cgcccagcga cgacgtcggc
     1501 gaccgcaccc gccaccgaca caccgttgac ctgtgcgccc accgacaccg cgtacgccgg
     1561 gatgccgtag acgaaattca ctgtgccgtc gatgggatca agcacccagg tgacggcgtc
     1621 cgcgggcgtt gcagtcggat cggtgggccc gccgccctcc tccccgagta tcgggtcacc
     1681 gggccgcagt tgcgccaacc tgtcccgcaa cagccgctcg gtctcggtat cgacgacggt
     1741 cacgggatcg gtcggggtgg tctttgcccg aaccacgtca ctggccgggg cggtcgaggc
     1801 acccgattcc gtgccgaata cctcggtgcg gcgacgccgg acgaactcgg cggcctcggc
     1861 ggccagcatc tcggctaccg accgcagccg agccggctcg tcgtcaggtc gcgtcaccgg
     1921 tctatcgcat cacaattcgc cgtggctccg cagacggcca acctgtaccg tcgccaagct
     1981 aaggtgagcc aacagccccg tctcgctgag gagtttggat gaccaccacc gactcgacca
     2041 cggatacgcc cagcaccgat gcacccaccg gcacaccgca gcgtcgtgga ttcggcatcg
     2101 acgtcggagg cagcggcatc aagggcggca ttgtcgacct ggacaccggg ttgttgatcg
     2161 gcgaccgcat caagctgctg accccccaac cggcgacgcc atcggcggtc gccaagacca
     2221 tcgccgaggt cgtcaacggc ttcgggtgga ccggtccgct gggggtgacc taccccggcg
     2281 tcgtcaccca cggcgtcgtg cagacggcgg ccaacgtcga caaggcctgg atcgggacca
     2341 acgccggcga cgtcatcggt gccgaactga acggccaagc cgtcacgatc ctcaacgatg
     2401 ccgacgcggc cggcctcgcc gaggcgcgct acggcgcggg cagggatcag tccggcgtgg
     2461 tcgtgttgct gacgttcggc acggggatcg gctccgcggt catccacaac ggcaccctgc
     2521 tacccaacac cgagttcggg catctcgagg tcgggggcaa agaggccgag caacgggcgg
     2581 catcgtcggt caaagacaag aacgactgga gctacgaaaa gtgggccaag caggtcacga
     2641 aggtgctggt ggccatcgaa aacgcgctgt ggcccgacct gttcatcgcc ggcggcggga
     2701 tcagccgcaa ggcggaccaa tggctgccgc tgctagaaaa ccgcacccgg gtggtggcag
     2761 ccgacctgct gaacaccgcg gggatcgtcg gcgcggccat ggcgtccaca tccgacgtga
     2821 cgcactgaat ttcccccgct gcggcggtac cagcgcggac tgtcgttaca atggttcacg
     2881 gcggccgccc gactgatagc gtgcgagtac tcgcgctgag atcaacgccg acattcgaca
     2941 tagcagacac tttcggttaa ccatgcccag acccaccgaa agtgcgtccg accaaagggg
     3001 tgtatgtggc agcgatcaag gcaagcccgg cgacagatga gccggtgaag cgcaccgcca
     3061 cgaagtcccc atcgtctccc gccaagcgat cggcggccaa ggccgccaac ggctcgaccc
     3121 cggcaaagcg gacagccaag tcggcatcgc cggccgccaa gcccgcgccg ggacacgagg
     3181 ccaccgcgcc cgacgaactt agcgaagcga caaagaagac ccgtgcgtcg gccaaggccg
     3241 gtggtgcgaa ggcgtcgtcg gcccgcggca caaaagcggc agacaacgat gctccggacg
     3301 accccgacgc cagcttgcaa cccctcgtcg ccgacgcggc cgtcgaggaa ctcgacgccg
     3361 aaccggacct cgaggatgag cccggcgtgg acatcgacat cgacgccgac gagctcgtgc
     3421 tcgatgatct cgaggacgac gtagccgccg acggcgacga agccgacctg gacccggctg
     3481 acgtcgagga agccgaggcg gctgaggccc ccttggccgt caaggccggc aaggctgcca
     3541 ccgccgagga cgaggagata gctgagccca ccgagaagga caaggcctcc ggcgatttcg
     3601 tctgggacga agacgagtcc gaggcgttgc gtcaggctcg caaggacgcc gagctcaccg
     3661 cctccgccga ctcggttcgc gcttacctca agcagatcgg caaggtggcg ctgctcaacg
     3721 ccgaggaaga ggttgagctg gcgaagcgca tcgaagccgg cctctacgcc acccagctga
     3781 tgagcgagca cgccgagcgc ggcgacaaac tgcccgccgc ccagcgccgc gacatgatgt
     3841 ggatctgccg cgacggcgac cgcgcgaaga accacctgct ggaagccaac ctgcggctgg
     3901 tggtttcgct ggccaagcgt tacaccggcc gcggaatggc cttcctcgac ctcatccagg
     3961 aaggcaacct gggcctgatc cgcgcggtcg agaagttcga ctacacaaag ggatacaagt
     4021 tctccacgta cgcgacgtgg tggatccgcc aggccatcac ccgcgccatg gccgaccagg
     4081 cccgcaccat ccgcatcccg gtgcacatgg tcgaggtgat caacaagctc ggccgtatcc
     4141 agcgcgagct gctgcaggat ctgggccgcg agcccactcc tgaagagttg gccaaagaga
     4201 tggacatcac gcccgagaag gtgctggaga tccagcagta cgcccgcgag ccgatctcgc
     4261 tggaccagac catcggcgac gagggcgaca gccagctggg cgacttcatc gaggacagcg
     4321 aagcggtggt ggccgtcgac gcggtgtcgt tcactctgct gcaagaccaa ctgcagtcgg
     4381 tgctcgagac gctctcagag cgtgaagccg gcgtcgtacg gctgcgcttc ggactgaccg
     4441 acggccagcc tcggaccctc gacgagatcg ggcaggttta cggcgtgacc cgcgagcgca
     4501 tccgccagat cgagtcgaag acgatgtcga agctgcgcca ccccagccgt tcccaggtgc
     4561 tgcgcgacta cctggactaa acacgccgaa tcgacggccg cgcaggccag acctgttggt
     4621 gcgcaagcca agtcatgacc cctacgcact gcggccgcgc cgagtgtgcc gccgccgtag
     4681 tgtgaccgca tgccggcaaa ccgcaaactt gtctcctccg aatccgactt cgaatcgacg
     4741 gtcggctatt cgcgggcggt gcgcgtcggc ccgcacgtgg cagtggccgg gacaaccggc
     4801 gccgggcccg ccggcgacat ttcggctcag acccgagacg ccttgcgccg catcgagatt
     4861 gcgctgcagc aggccggcgc ggcgctcacc gacgtggtcc gcacccgcat ctacgtgacg
     4921 gacatttcac gctggcgaga ggtcggagcc gcgcatgcgc aggtgttcgg tgcgattcgc
     4981 ccggcggcca gcatggtgga agtttccgcg ctgatcgagc ccgatttgct ggtggagatc
     5041 gaggccgacg cctacgtcga gtccgagggc gggtccccag ccgcgttcga cgttcgcggg
     5101 accggcggga acgtgccatc ggctgccggg gggtaggcgg tgacgttgag caccgcgcgc
     5161 accggcagcg ggccgtacag atgcggaaac agcatcgact ccggatccgt tgccaccccc
     5221 ggctcccagc gcacgggcga gtccagcagc gccggattga tgtgcagcag aaccaggtcg
     5281 ccgcgaccgc ggtagagccg gttggccggc aggtggactt gctcgggcgt cgacaggtgg
     5341 atgaaggccg tggcgttcga gccggaggcc ggggcgttcg gggggcgaat ctcgccgcgg
     5401 ctgcgggccc gggaccactc ctcggcccca cacaggtgca ccagcacgtc ggcagcgatg
     5461 ttcacagtcg gccaccttag agaacccggc gggacggccg tgagaaatcc gtgagaaacg
     5521 acacacccga caacgatcgg ggaacaacgc gaaaacccca tacgtctgag aaagtggatg
     5581 tacgagaacg gagaggccat gaatgcaact ctgaccagtc ccgagctgac cagggcggac
     5641 cgctgcgatc gctgcggtgc tgcagctcgc gtccgtgcca agttgccttc gggactcgag
     5701 cttctgttct gccagcacca cgccaaccag cacgaggcga aacttgccga gctggccgcc
     5761 gtgatcgagg tcagcgcgag ctagtcgacc caactcaccc caggcaatgt gggggccgcg
     5821 ggccctcatg ggcaatgctt gcgactatga acgctcaggt cgcaaagccg acacgccacc
     5881 atatttggcg aatcagcctt aggaccctgt cgaaaagctg ggacgattcg atcttctccg
     5941 aagccgcaca ggcgggattt tggtcggcgc tgtccctgcc gccgctgctg ttggggatgc
     6001 tgggcagctt gacttacgtg gccccgctgt tcggcccgga caccctgccc gagatcgaaa
     6061 agaccatcgt ttcgacggct cacaagttct tctcccccag cgtcgtcaac gagatcatcg
     6121 agcccaccat ccgcgacatc accgccaacg cccgcggcga ggtggtgtcc ctgggtttcc
     6181 tgatctcgat gtgggccgga tcgtcggcaa tctcgtcgta cgtcgacgcg gtggtggccg
     6241 cccacgatca gactccgctg cgacatccgg tgcggcagcg attcttcgcg ctgttcctct
     6301 acatggtgat gcaagtgttt gtggtggcaa tcgcgccgat cattgtgctg ggcccgcgca
     6361 agctgcgaga acacataccg gccggcctgg ccaacatgct gcattacggc tactaccctg
     6421 cgctggtgct cggcctgatg atcggggtca tcgtcttgta ccgggtgtcg ctccccgtac
     6481 cactgccgac gcatcggctg atctttggcg ccgtgctggc gaccgcggtg ttcctgatcg
     6541 ccacgctggg actgcggatc tacctgggct ggattaccag caccggctac acctacggcg
     6601 cgctgtccac gccgatcgcg ttcctgctgt atgcgttctt cgcgggcttt gccctcatgc
     6661 tgggcgccga gctcaacgcc gccatccaag aggaatttgc cgcaccggcg acccatgccc
     6721 accggttgcg cagctgggtg atctcgcgcg tgcccgcgct gcagcgaacg gtcgactcgc
     6781 caacggaacc cgcacttccg cggcgcgacg tcgcgacggc cgagccgccg acctgatcag
     6841 cccttcttga gctgttcgta gatgcgcttg cagtccgggc agaccgggga acccggcttg
     6901 gcggcccgag tgacgggaaa tacctcgccg cacaacgcca ccacatggct gcccatcacc
     6961 gcgctctcgg cgatcttgtc cttcttgacg taatggaagt acttcggagt gtcgctgccc
     7021 gtcccgtcgt cgacccgttc gtcggtctcg gtgcgctcga tggtctgcgt ctgcatacct
     7081 cacattctgc actgcggagc ccgctcgaaa accggctgag ccggaatcga tgaggtgtgg
     7141 gacagtggag gaatgaagcg ccgctctcac aagcggttcg acgacggatt cgatgacttc
     7201 gacgacaagg gccagccggt gctcatcacc gccgctgcgc cctcctacga agaggaacag
     7261 cgcacccgga tccgtaagta cctgactttg atggcgttcc gggtcccggc gctcatcctg
     7321 gccgccgtcg cgtacggcgc ttggcacaac ggcctgatct cgctggcgat agtggcggtg
     7381 tcggtgccgt tgccgtggat ggccgtgctg atcgcgaacg accgtccccc tcgccgcccc
     7441 gaagagccgc ggcggttcga cgctgccccg cgacgcactc ggctgttccc gagggccgag
     7501 ctgccggcgc tcgatcctcc gcgacacccc gagccacctc cggactggga tagtcgcgaa
     7561 tagtcgccgt tgccgcgggc cgccaaggtt ccgggcaacc acccggccgt cgcacttctc
     7621 aggacattct caggtcgtcg gcacatttct gcacgtcagg gcgtgtgaga tggcgaccgg
     7681 gtgggaactc ccaggctgga tctgtcgtta aaccacatga cagaacaaag cctaagggga
     7741 ggccgctatg gcgaatgcca ccacaagcag attcgacggc gatctggatg ctcaaagccc
     7801 cgcagcggac ctggtgcgcg tatatctgaa cgggattggc aagacggcgc tgctcaacgc
     7861 agcaggtgaa gtcgacctgg ccaagcgcat cgaggcgggg ctctatgccg accatctgct
     7921 ggaaacgcgc aagcgcctgg gcgagaaccg caaacgcgat ctcgaggccg ttgtgcgcga
     7981 tggcgaggca gcgcgccgcc atctgctgga agcgaacctg cgcctggtcg tgtcgctggc
     8041 gaagcgctac acgggtcggg gaatgccgtt gctggacctc atccaggagg gaaacctggg
     8101 tttgatccgc gcgatggaga agttcgacta cacaaaggga ttcaagttct cgacgtacgc
     8161 gacgtggtgg atccgtcagg ccatcacccg cggcatggcg gaccagagcc gcaccatccg
     8221 tctgccggtc cacttggtgg agcaggtcaa caagctggcg cggatcaagc gcgagatgca
     8281 ccagaacctg ggacgtgaag ccaccgacga ggaactggcc gccgaatccg gcattccgat
     8341 cgagaagatt aacgacctgt tggagcacag ccgcgacccg gtgagcctgg acatgcccgt
     8401 cggctcggag gaagaggccc cgctgggcga cttcatcgag gacgcggaag cgatgtcggc
     8461 ggagaacgcg gtgatcgccg agctgctgca caccgacatc cgcagtgtgc tggccactct
     8521 cgacgagcgc gagcaccagg tgatccggct gcgtttcggt ctggacgacg gccagccgcg
     8581 aaccctggat cagatcggca agctgttcgg tctgtcccgc gagcgggtcc gccagatcga
     8641 gcgcgacgtg atgtgcaagc tgcgcagcgg cgagcgcgcc gaccgcttgc ggtcgtacgc
     8701 gagctgaggc cgcgaaccag agccaggtag ccagacggta tgcccgccgc gttcagcggc
     8761 gggcatactg cttgcagcag gtagcgttac acgcgtatac gcagccggcc aagtagactc
     8821 agcgtcaatg gagggtgctg aatgaacgag ctggtcgata ccaccgagat gtacctgcgg
     8881 accatctacg acctcgagga agagggcgtg acgccgctgc gtgcccggat cgccgaacgc
     8941 ctcgaccaga gcggcccgac cgtcagccag accgtatccc gcatggagcg ggatggatta
     9001 ctccacgtgg ccggcgaccg ccatctggaa ctcaccgaca agggccgcgc gctggccatc
     9061 gccgtgatgc gtaagcaccg cctggccgag cggctgctcg tcgacgtcat cgggctgccg
     9121 tgggaagagg tgcacgccga ggcgtgccgg tgggaacacg tgatgagcga ggacgtcgag
     9181 cgccggctgg tcaaggtgct caacaacccg accacctccc cgttcggcaa cccgatcccc
     9241 ggtctgctgg acctgggcgt gggtcccgag tccggcgctg acgacgcgca cttcgtccgg
     9301 ttgacggagc ttcccgccgg gtctccggtg gcggtggtcg tccgccagct caccgaacat
     9361 gtccagggtg acatcgatct gatcacgcga ctcaaggacg ccggcgtcgt tcccaacgcg
     9421 cgggtgacgg tcgagaccgg cccggccgcc ggggtcacca tcctcatccc cggccacgag
     9481 aacgtcacct tgccccacga gatggcccac gccgtcaagg tcgagaaagt ctgacccgct
     9541 cgcgacggtc acgaccggcc actagcgcaa atggtggcga cccgcttcgc ccggctccgc
     9601 cgcgctcgcg atcgccacta ccctgcccgg cggccgaacg cccgtcgcgg cggtaggcgc
     9661 acgccgagcc gcttggccac ccgatagccg gtgacggcca gttcccggat ctgctcgggc
     9721 cgtaggccgg actgcaacgc cgtgtccagc atgcccgcca tccgatgctc ggaatcgcag
     9781 cgcagcgcgg cctctagcga tacgcccgca agcggtccgt caccgcgggc gtatgcgctg
     9841 aacgccagca gcaccaacgc ctcgacgcgc cagggttcgg gcagggtccg cgccagcagc
     9901 gcccacaatg actcggcttc gccggcgatc tcgccgaccg cgagcgcata cagcgtgtcg
     9961 cgcaccagca cgtcgctcag tgcgcagccc agctgcgcca gctccgcgtc cgacagcgga
    10021 tggccgcccg ccacccggcc ggcggcggcc atcgcgtctt ccacatcgcg gcggctgcac
    10081 cccgcgggat ccgcgcgatg cgccgcctcg cgtgcgcccg cccgttgccg catcgaagac
    10141 gccaactcgg cgctgcgcgc cccgtcgtcg gcggcgatga cggcctgcag gtcggcgcgc
    10201 cgcgggtaga gccgcctgcc gtccagcacc gcggcagcgg ccaacggcga cgcggacggg
    10261 tcgtccaccg cgccgctcga gccgcagccg tccacgcaat gccagcgccc gccgcgggcc
    10321 acccgatcca ccacgtgcgc ggcccacagc tcaatgtcgt acgcggacaa cgcgtctgcg
    10381 agcgtcgcgc acagctgccg atagtcctgg ttgcaccggg gacagtgcgc gccgtcggcg
    10441 gcgacgatca ctgcgatcgc ggcttcgggc tcggccgcgg cggcgacctc ggcgaggtgg
    10501 ccgacccggt ccacgagctc ctcgccgagg tcgacccgca gcaccgaccc cagttcgccg
    10561 ccggtcaatg acaccagtac cagtgaattt tccggaacga agccgagaac ggccggtagc
    10621 gcggcgatca atgccccggg gcggttcagt tcacaatctc ctcgatgcgt cgtcatgagc
    10681 gtcaacgctg acggcggcca ccgtcagctt gtgcgcacgt gaccgggctg ggccacccgt
    10741 ctgtggagaa agtctcgact gtgggcctta tcgtctaacc atgggttcga tgcaggagta
    10801 cgacatggtc gtcatcggtt cggggccggg cggccaaaag gccgccatcg cctcggccaa
    10861 gctagggaaa tccgtcgcca tcattgaacg cggccgaatg ctcggcggtg tctgcgtgaa
    10921 caccggcacg atcccgtcaa aaacgttgcg cgaggcggtg ctttacctca ccgggatgaa
    10981 ccagcgcgag ctctacgggg cgagctatcg cgtcaaagac cggatcaccc cggccgatct
    11041 gctggcgcgg acccagcacg tgatcggcaa ggaagtcgac gtggtgcgta accagttgat
    11101 gcgcaaccga atcgacctgc tgatcgggca cggccggttc ctcgacccgc acaccgtcgt
    11161 cgtcgaagac cccggccgcc gggagatgac caccgtcagc ggtgaataca tcgtcatcgc
    11221 caccggcacc aggccggcgc ggccgtccgg agtcgagttc gacgaagatc gggtgctcga
    11281 ctccgacggg atcctggatc tcaagacgct gccggcctcc atggtcgtcg tcggcgccgg
    11341 ggtgatcggc atcgagtacg cgtccatgtt cgccgcgctg ggcaccaagg tcaccgtggt
    11401 ggaaaagcgg gacgacatgc tggacttctg cgaccccgag gtggttgagg cgctgaaatt
    11461 ccacctgcgc gacctggcgg tgacgttccg gttcggtgag gcggtgaccg cggtcgacgt
    11521 cggctcggcg ggcaccgtca ccactctggc cagcggcaag cagattcccg ccgagaccgt
    11581 catgtactcc gcggggcgcc agggtcagac cgatcacctc gacctgcaga acgccggcct
    11641 cgaggtcgag ggtcgcggac ggatctgggt cgatgacaga ttccagacca aggtggagca
    11701 catctacgcc gtcggcgacg tgatcggctt ccccgcgctg gcctcgacct cgatggagca
    11761 gggacggctt gccgcctacc acgccttcgg ggaagcctgc gagggcatga ccgacctgca
    11821 gccgatcggc atctattcga tccccgagat ctcctacgtc ggtgccaccg aggtggagct
    11881 gaccaagaac gcggttccct acgaggtcgg ggtggcgcga tataaggagc tggcccgcgg
    11941 tcagatcgcc ggcgactcct acggcatgct caagctgctg gtgtccaccg aggaccgcaa
    12001 gctgctcggg gtgcacatct tcggcaccag tgccaccgag atggtgcaca tcgggcaggc
    12061 cgtgatgggt tgcggcggca ccgtcgacta cctggtcgac gcggtgttca actttccgac
    12121 gttctccgag gcctacaagg tcgccgcgct ggacgtgacg aacaagatgc gggcgctgag
    12181 ccagttccgt cgctgaccgg gccgccctac gagcagctgt cgtcgggtcc gttcggcacc
    12241 atgtcgcccg gaccgccggc ctcggcgcgg acgagtaact gcgcggtttg ttcgtcgaac
    12301 ggcgccgagt acgacacgtt ctcggcgcac ccgccgcgct cgaaaccacc gatcaccccg
    12361 gtgagcgtgg taccgctgac ccacggcccg ccgctggtgc cgcccaccag cccttcgcac
    12421 accaacgagg gaaatccgct gtcgttgacc gaggtgctgg tctgacagcc gatgggcgag
    12481 ccgcccaccc cggccggata ccccatcacc gtgacgtgac tgccgggcgg gggggccacg
    12541 cccaacgtca gcgccaggcc gacgtgtgac tcgaccgaac caccgccggt gctgctgacc
    12601 cgcacgatcg cataatcggc gcggggatcc ctgctggcgc tccagcgggg atcgaggtag
    12661 acggcatcag cggtccacat gtcggggggc gcggcggtac cggcgaaacc gggaacgaag
    12721 gtgatgcggg acgccccggc caggcagtgt gccgcggtca tcaccaggtt cccattcccc
    12781 gaatgcacga cggaacccgt gcaaacatgc acgttgcccc cgtccaggaa gatcgcgccc
    12841 acgcgcctgt ccgggtccac cggacccgcg accgcctgct gctggggctg gctgagatgc
    12901 tgaaccggcc cgctgacccg cgacggggac acggggggga tcgatgcgtg ctcggcgggt
    12961 cgcgtgcacg acgtcaccaa cgtcgcaagg ccgaccaccg ggcaaagcaa tacgaaaggc
    13021 acgcgcattt ggtgtccatc atgcccgacc ctagggtggc aagcagtgct ggacgcagca
    13081 ccgtgtgacg acgggtttgc tggctaattt gccaccgccg aaaatatcgg atacggatta
    13141 cgttccggcg ttaccgcgtt tcagggccta aatgcagaca cggttcgctg tttgatgttg
    13201 ctgcccgttg attcggggga cactggtaag ggcaccctcg agagaccccc gagaggagga
    13261 aaccctgatg gcccacgatc aaaacccgga cgattaccag ccaggacaac ccgggctgta
    13321 cgagctggag ttcccggcgc cccagttgtc gacgaacgac ggccgcggcc cggttctggt
    13381 gcatgccttg gagggcttct ccgacgccgg tcacgcgata cggctgtccg ccacgcacct
    13441 gaaggcctcc ctggacaccg agctggtcgc gtcgttcgcg atcgacgaat tgctggatta
    13501 ccgctcgcgg cggccgctga tgaccttcaa gacggatcac ttcaccagct acgaggatcc
    13561 agagctgagc ctgtatgcgc tgcgcgacag tgtcggcacc ccgttcctgc tgctggccgg
    13621 catggagccg gacctgaaat gggagcgctt tatcaccgcc gtacgcctgc tggccgagcg
    13681 gctgggtgtg cgccggacca tcggcctggg caccgtcccg atggcggttc cgcacacccg
    13741 gccgatcacg ctgaccgcgc attccaacga cggtgaactg atcgccgatt tccagccctg
    13801 gatctcggag atccaggtgc ccggcagcgc gtccaacctg ctggaatacc ggatggccca
    13861 gcatgggcac gaggttgtcg gtttcaccgt gcacgtcccg cactacctga cgcagaccga
    13921 ctaccccgcc gccgcgcagg cgttgctcga gcaggtggca aagaccggat cgctggagct
    13981 gccgctgtcg gcgctgtccg acgcggcggc agagatccgg gccaagatcg acgagcaagt
    14041 acaggcgagc gcggaagttg ctcaagtggt ggctgcgctc gagcgccagt acgatgcctt
    14101 catcgacgct caggagaaca ggtcgttact aactcgcgac gaggatctgc ctagcggaga
    14161 tgagctgggt gcggagttcg agcgattcct cgcccaagag gcaagcaaga agtccgagga
    14221 cgacgaccag acgtaagtcg tcgaaggttc tcggaacgcc gatagcccga cccgagacaa
    14281 gggtggcgag atgaccgagc ggaagcgcaa gagcaacctt cgcccggtcc gtgaggtgac
    14341 gaccccgagg ctggagtttc gcaccatcca tggttaccag cgggcctacc ggatcgccgg
    14401 ttccgggccc gcgattctgc tgatccacgg catcggcgac aactccacca cctggaacgc
    14461 cgtgcaggcc aagctcgctc agcgattcac ggtcatcgcc ccggatctgc tgggccacgg
    14521 gaaatccgac aagccgcgcg ccgactactc ggtcgcggcg tacgcaaacg ggatgcgtga
    14581 cctgctcagc ctgctcgaga tcgaacgggc gaccatcatc ggtcattcgc tgggcggcgg
    14641 cgtcgcgatg cagttcgcct accagttccc gcatatggtc gagcggctga tcctggtcgc
    14701 ttccggtggc gtcaccaagg acgtcaacct ggtcctgcgg ttggcctcgc tgccgatggg
    14761 cggcgaggct ctggcattgc tgcggctacc tctggtgctg ccggcgattc agctggcggg
//
LOCUS       DASSGQ010000004         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_698974,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000004 DASSGQ010000000
VERSION     DASSGQ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698974"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFI55_00155"
     CDS             <1..211
                     /locus_tag="VFI55_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072482.1"
                     /translation="LIEAGTSVTPPGQMAAMTKTSSPTTYLCVQQSGHLIHDEAPQAY
                     REAVESFLSVTGRRRARARSAAPPP"
     gene            complement(162..1865)
                     /locus_tag="VFI55_00160"
     CDS             complement(162..1865)
                     /locus_tag="VFI55_00160"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET7072483.1"
                     /translation="MPATKAIIVGSGAGGSTVAMELAENGWDVVIFEKGSNYFTNLEG
                     TGPLGTTFSNDELKAVYRYFEQPDPLAYPRTYRTSAAQAAGYIGPVNELPVTVGGGTT
                     HWDAKVPRFWDIDFKGLSLLGPQPGADVQDWPFEYSDIAPYYDAIEALLGTQGDVGAM
                     PALTLKHAPRSRPFPMPPGPQQRSSTLIAKGARRIGLHPFPFPSAINSVEFDGRPACN
                     NCGFCANYGCPISARVGALAPLRRALRTGRVQLRPETTATKVNFIGRRATGVSYLDPQ
                     GNAGGESADVVVLAASAIETARLALLSGLPDASGRIGARLMFHNFVDGFGIYLDQRVH
                     AYRGRSITQCMEDFCNPDFPGARAAARLAGLPYIRGGLCELGGSQDPITEGQFYQEIL
                     GFLPGVQLFGSPFKDLMRASLLRDRLAGVNFIGTDLPYLTNNVTLDPSVTDLNGQPVP
                     RITYAPGKHEAVAQTFYLLPITAMMAAAGATLFAAVPDGLERKLGGGTPPNTKHVMGG
                     MQMGVSPATSVVDPYGRMHQMDNVFVADGSVFVTSGCHNPTNTLMAVALRIGRGLAGG
                     R"
     gene            complement(1852..>2046)
                     /locus_tag="VFI55_00165"
     CDS             complement(1852..>2046)
                     /locus_tag="VFI55_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072484.1"
                     /translation="NANLVGWLDIKFPGDSQPRGYTAGEVEAAQWDLVVVSDVVTRLR
                     HGWEQLIAKLGGKGGVGAGH"
BASE COUNT          328 a          667 c          697 g          354 t
ORIGIN      
        1 gctcatcgag gcgggcacct ccgtcacccc gccgggccag atggcagcga tgaccaaaac
       61 aagttctccg acaacgtatt tgtgtgttca gcagtccggt cacctgattc acgacgaagc
      121 gccgcaggca taccgcgagg cggtcgagtc gttcttgagc gtcaccggcc gccggcgagc
      181 ccgcgcccga tccgcagcgc caccgccatg agcgtgttgg tggggttgtg gcagccggag
      241 gtgacgaaca cgctgccatc ggcgacgaag acgttgtcca tctggtgcat tcgcccatag
      301 ggatcgacga cgctggtagc cggggacacg cccatctgca tcccgcccat gacgtgcttg
      361 gtgttcggtg gagtgccgcc cccgagtttc ctttccagac catcggggac ggcggcgaac
      421 agcgtcgcgc cggctgccgc catcatcgcg gtgatgggca acaggtagaa cgtttgcgcc
      481 accgcctcgt gctttccggg ggcgtaggtg atccggggca ccggctggcc gttgaggtcg
      541 gtgacactcg ggtcgagagt cacgttgttg gtcaggtacg gcaagtcggt gccgatgaaa
      601 ttcacgccgg cgagccggtc gcgcaggagc gaggcgcgca tcaggtcctt gaacgggctg
      661 ccgaacagtt gcacgcccgg caggaatccc agaatctcct ggtagaactg gccctccgtg
      721 atcgggtcct ggctgccgcc caactcgcac agcccgccgc ggatgtaggg cagtccggcc
      781 agtctcgccg ccgctcgagc cccggggaag tcggggttgc agaaatcctc catgcactgc
      841 gtgatcgagc ggccgcggta ggcgtgcact cgctgatcga gataaatgcc gaacccgtcg
      901 acaaagttgt ggaacatcag ccgggcgccg atccgaccgc tggcgtcggg caggccggac
      961 agcaacgcca ggcgtgctgt ctcgatcgcc gacgcggcca gcaccactac atcggcgctt
     1021 tctcctccgg cattgccctg agggtcgaga taggagacgc cggtggcccg ccggccgatg
     1081 aagttgactt tggtggcggt cgtttcgggg cgcaactgca cccgcccggt ccgcagcgcc
     1141 cgccgcagcg gcgcaagcgc gcccacccgc gcggagatcg ggcagccgta attggcgcag
     1201 aacccgcagt tgttgcatgc cggacggccg tcgaactcga ccgaattgat cgcgctgggg
     1261 aacggaaagg gatgcagccc gatacgcctg gctcccttgg cgatgagggt ggacgagcgc
     1321 tgttgcggcc cgggcggcat cgggaagggc cgcgagcgcg gcgcgtgctt gagggtgagc
     1381 gcgggcatcg ccccgacgtc gccctgagtt ccgagcaggg cctcgatcgc gtcgtaatac
     1441 ggggcgatgt cgctgtattc gaacggccaa tcctgcacgt ccgcgccggg ctgcgggccg
     1501 agtagcgaga gtcccttgaa gtcgatgtcc cagaatcgcg ggaccttcgc gtcccagtgc
     1561 gtcgttcctc cgccgacggt taccgggagt tcgttgaccg ggccgatgta gccggcggct
     1621 tgggcggcgc tggtgcgata cgtgcgcggg taggcgagtg ggtccggttg ttcgaagtat
     1681 cggtacaccg ccttgagttc gtcgttggag aacgtcgtcc cgagtggccc ggtcccctcc
     1741 agattggtga agtagttcga tcccttctcg aatatgacta cgtcccagcc gttctccgcg
     1801 agctccattg ccaccgtcga cccgccggcg ccggagccga cgatgatcgc cttagtggcc
     1861 ggcacctacg ccaccctttc caccgagttt ggcgatcagc tgttcccagc cgtgacgcag
     1921 ccgggtgacg acgtcgctca cgacgaccaa gtcccactgc gccgcttcga cctcgccggc
     1981 ggtgtatccc cgcggctggg agtcgcccgg gaacttgatg tcgagccacc cgaccaggtt
     2041 cgcgtt
//
LOCUS       DASSGQ010000005         3494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_790150,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000005 DASSGQ010000000
VERSION     DASSGQ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790150"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1691)
                     /locus_tag="VFI55_00170"
     CDS             complement(<1..1691)
                     /locus_tag="VFI55_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072485.1"
                     /translation="MNDVPSAIRATSSEVPPPVSTENRLRPAENVAVHVLSSVAAFVA
                     LACAVLVLNGGPSKKLDLPLWVMAFAIALPLGTFLGARQDRRLAAIPQPLARWAVAFG
                     TSLLMFGLVFLRLLHTGSAQLTVLGFLALAAYGVTALAARRPAIFSRLEARPAWAPVV
                     VGVSAIVIMTGFFLVTPITRWALVIGRPFPPLVVVGIALTAVLVLAGAALAGVLVLDR
                     QTPRPWRRRLFDVGLSVVLAMVVFQVKLPTPLDHSVVVSVVHHDFYLASVNDMTHGRT
                     MLVDMWALYGVGVYYALLAALRVLPFNHGGLVLLLSILMTAQYVLVYATLRTAVRSQA
                     LVIAAVGASVMGNIFAAVDSYVVYPSIGPLRFGPPYLVVAAAVAAARWPQYARAMRVG
                     QLVVIAIAAVWSFETFVYTAVTWFALAALLAFGRARAGLWVFVRELAAAAAVSVGAVL
                     ALTIGARVVAGAWPNWGGYFAHVLLYSEGGYGLLPFDFWSPALLMAAVIFLSAVGVVS
                     VARDERVQASGPVLAGLAGFTGFAASSFTYYVNRSHPNALLVLFLPICALGFLWA"
     gene            complement(2114..>3494)
                     /locus_tag="VFI55_00175"
     CDS             complement(2114..>3494)
                     /locus_tag="VFI55_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020725030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072486.1"
                     /translation="FAHLLSAVLLALTASALFVAASRVIGREPALVATLVWWFPIAMT
                     FPVGGPNDFSALSTEYLPILLIVASALVPREQLAARPWLFAVLGVLAGIAVGAKYQVG
                     PLAAAWVAAQLIVLRPSVRRIAVSVLWWLVGAVLPAAAILLVMVASPATNWTIVEQIV
                     SFLTSYTGSRSPLQRVGSTFAALIGPGCYLLVALAGLIWLSLHSERRSNVARAVLVTG
                     GLTAMLGGPAGGVGVQTPGGFPHYLIILFGAVGLAFTMPVKPGAQLIPQRLSPTVLAG
                     ALATVAALVLVNGLITDRWAPLSPRGAAAAFSSNSVNRNPELARACPPGSRALVWGWK
                     NELYVAQDWKGTIPYPNVLNLSMSPANRETGEPVIREGIERADCVVETTSICSGCPRL
                     PAELTLPRYYPELGELIGRQFHTVPVGGCDGCTLYVRNVSSRASVAPRRKKKKCGGQR
                     TSAARRTPR"
BASE COUNT          719 a         1155 c         1170 g          450 t
ORIGIN      
        1 ctcgcccaaa ggaagccgag ggcgcagatg ggcaggaaca ggacgaggag ggcgttcgga
       61 tgcgagcggt tcacgtagta cgtgaagctg gacgcggcga agccggtgaa cccggcaagt
      121 ccggcgagta ctggtccaga ggcttggacg cgctcgtcgc gtgccaccga cacgaccccg
      181 actgcggaca agaagatcac ggccgccatc agcagggcgg gcgaccagaa gtcgaaggga
      241 agcaagccgt atccgccctc cgagtacaga aggacatggg cgaagtagcc gccccagttc
      301 ggccacgcac cggctacgac gcgcgcaccg atcgtcagcg cgagcacggc cccgactgag
      361 acggcggccg cggcggccag ctctcgcacg aacacccaca gccccgcccg cgcccgcccg
      421 aaggccagca gggcggccaa tgcgaaccac gtgacggccg tgtacacgaa cgtctcgaag
      481 ctccacaccg cggcgattgc gatgacgaca agttgtccta cccgcatcgc ccgcgcgtat
      541 tgcggccaac gcgcggcggc gaccgccgcc gccacgacca ggtacggggg cccgaagcgc
      601 aggggcccga tgctggggta caccacgtac gaatcgaccg cagcgaagat gttgcccatg
      661 acggacgctc ccacagccgc gatcaccaac gcttgggacc ggactgcggt gcgcagcgtg
      721 gcatagacga ggacgtactg cgcggtcatc aggatgctca ggagcagcac gagaccgcca
      781 tggttgaatg gcagaaccct cagggcggcg agtagcgcgt agtagacgcc gacaccgtac
      841 agcgcccaca tgtcgacgag catcgtgcgc ccgtgcgtca tgtcgtttac cgacgccagg
      901 tagaagtcgt ggtggacaac agacacgacg acggagtggt cgagtggcgt aggcaacttc
      961 acctggaaca cgaccatcgc cagcacgacg gagaggccca cgtcgaacag gcgacggcgc
     1021 caaggccgcg gcgtctgccg gtcaaggacg agcacgccgg cgagggcggc ccccgccagc
     1081 acgagcacgg cggtgagggc gatcccgacc accacgagtg gcggaaaagg tcggccgatc
     1141 acgagcgccc accgggtgat cggggtcacg aggaagaatc cggtcatgat cacaatcgcg
     1201 ctcacgccga ccacgacggg cgcccaggcc ggccgtgcct cgagcctgct gaagatcgcc
     1261 ggacggcgag cggcgagtgc ggtgacccca tacgccgcca acgccaagaa ccccagcacg
     1321 gtgagctgcg cgctgcccgt gtggagcaac cggaggaaca cgagcccgaa catcagtagc
     1381 gaggtgccga acgcgaccgc ccagcgggcg agcggctgcg ggattgcggc gagccggcga
     1441 tcctgacggg caccaagaaa tgtgcccagg ggaagcgcga tcgcaaaggc catgacccat
     1501 agcggcaagt caagcttttt ggacgggccg ccgttgagca ccaacaccgc gcaggcgagc
     1561 gcgacgaagg cggccaccga ggacaagacg tggacggcga cgttctcggc ggggcggagc
     1621 cggttctcgg tgctgacggg cggcggcacc tctgaactcg tcgcgcgtat cgccgagggt
     1681 acgtcgttca ccgtcgacac accgtccggg tgccctgcgg catctggcac gtggcaaaca
     1741 agagatcccc cctcgttcac gatgcataga actagaaacg gaaggtagca aaccttacgt
     1801 ttcaccggac gcttttgtct gccgcgggcc gaagaacagg tcgaacacgg tcaaatcttg
     1861 cgttaacgag ggcgattacc cggcacaacg cagtcaggtg taattcggcg cagtcgcgcc
     1921 gatgtcgtcg acggcactac gagttcctcg accatctgac attaagagcg aacggagttg
     1981 ctgcccgacg gcaaggccat gaagctcatt gcgagggaga aagaccgatg agttcgaagc
     2041 gatcctgccc cgtctgttgg cccgattgga acagttcagc caacgcctct gcaccgcatc
     2101 ccggcgcatg caatcatcgc ggcgtccgcc gcgctgctga agtcctttgg ccaccgcact
     2161 tcttcttctt cctccgtggc gctaccgacg ctctggacga gacgttacgc acatacagcg
     2221 tgcagccgtc acagccgccg acgggcacgg tgtggaactg ccgaccgatc agctcgccga
     2281 gttctgggta gtaccgcggc agggtcaact ccgccggaag acgggggcat ccggagcaga
     2341 tactggtcgt ttcgacgacg cagtcggccc gctcgatccc ttcgcggatc accggctcgc
     2401 cggtctcccg gttggcggga ctcatgctca ggttgagcac gttcgggtag gggatcgtgc
     2461 ccttccagtc ttgtgcgacg tagagttcgt ttttccagcc ccacaccagg gctcgcgacc
     2521 cgggcgggca ggcgcgggcc agttcggggt tgcggttgac cgaattggac gagaatgcag
     2581 cggcggcccc tcgaggtgac agcggcgccc agcggtcagt gatgaggccg ttcacaagca
     2641 cgagggccgc cactgtggcc agcgccccgg ccagcacagt tggcgacaac cgctgcggaa
     2701 ttagctgggc gccgggcttg accggcatgg taaaggccag gccgaccgcc ccgaacaata
     2761 tgatgaggta atgcggaaaa cccccggggg tttgaacgcc tacaccaccc gcgggtccgc
     2821 ccagcatggc cgttaacccg cccgtgacga ggacggcgcg ggccacgttg gaccgtcttt
     2881 cgctatgaag gctcagccag atcaggccag cgagcgcgac gagcagatag caccccggcc
     2941 cgatgagcgc cgcgaaggtg cttccgaccc gctgcaacgg cgagcggctg cccgtgtagg
     3001 aagtcaggaa ggagacgatc tgctcgacga tcgtccagtt ggtcgccggc gaggcgacca
     3061 tgaccagcag gatcgcggcc gccggaagca cggcgcccac gagccaccac agcaccgaca
     3121 cggcgatgcg cctcacactt ggtcgcaaca cgatcagctg cgccgcgacc cacgcagcag
     3181 ccaacggacc gacctggtac ttcgcgccaa cggcgatgcc ggccagcacc ccgaggacgg
     3241 cgaacagcca gggccgcgcc gccaactgtt cgcggggcac cagcgccgag gccacaatga
     3301 gcagaatcgg caggtactcc gtgctcagcg cgctgaagtc gttcgggccg cccaccggga
     3361 acgtcatcgc gatcgggaac caccagacca gcgtggccac gagtgcgggt tcccgaccga
     3421 tcacccggga cgcggcaaca aacagggcgg aggcggtgag cgccagcaaa actgccgaca
     3481 gcaggtgagc gaag
//
LOCUS       DASSGQ010000006         5466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_547165,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000006 DASSGQ010000000
VERSION     DASSGQ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547165"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(296..2563)
                     /locus_tag="VFI55_00180"
     CDS             complement(296..2563)
                     /locus_tag="VFI55_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HET7072487.1"
                     /translation="MAIKEHDNGVSYVRTEKDLPPVAIIDHSPITIRHKIAFGVIALI
                     GAIAWAVIAFARGETVNAVWFVVAAICTYILGFRFYARLIEMKIVRPRDDRATPAEVV
                     DDGTDYVPTDRRVLFGHHFAAIAGAGPLVGPVLATQMGYLPCSIWIILGAVFAGGVQD
                     YLVLWLSTRRRGRSLGQMARDELGTTGGTAALIGTFVIMVIIIAVLALVVVRGLAQSP
                     WGVFSIAMTIPIALFMGCYLRFLRPGRVGEVSIIGFVLLMLAVASGHWVGETSWGTTW
                     LNLSPVTVSWLIIIYGFVASVLPVWLLLAPRDYLSTFMKVGAIALLAIGICIARPLMA
                     APAISRFASSGDGPVFAGSLFPFLFITIACGALSGFHALISSGTTPKLVEKESQMRLI
                     GYGGMLTESFVAVMALISASILDQHLYFTLNAPAAQTGGTAASAADYVNRLGLSGTPA
                     TADQLKQAAAAVGEKSIVSRTGGAPTLAVGMSEVLQRVFGGSALKGFWYHFAIMFEAL
                     FILTAVDAGTRVARFMLSDSLGNVGGPLAKLRNPSWRPGVWICSLAVAAAWGSILLMG
                     VTDPLGGINTLFPLFGIANQLLAAIALTVITVVVIKKGLLRWAWIPGAPLLWDLAVTL
                     TASWQKIFSADPAIGYWTQHSQYLAAKHAGKTAFGSAKNVHQINEVIRNTFIQGTLSI
                     LFAVVVVIVVIVGLVAALRAIRGGGMPLTEEDPSPSKRFAPSGMIPTAREREVQRQWD
                     ALPAPTIGLVGAQEL"
     gene            complement(2625..4184)
                     /locus_tag="VFI55_00185"
     CDS             complement(2625..4184)
                     /locus_tag="VFI55_00185"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET7072488.1"
                     /translation="MLLLDVASTSLRVGGTSSRLSKVSHIADLLRRAAAEPEAVAIVV
                     SWLSGELRQRQIGVGWASLRSRPPPREPGHPVLTVIGVDATFSEIGAVSGKGAQARRA
                     QLVAGLFGAATETEQTFLLRLLGGELRQGALAGIMADAVARATGLPAAAVQRAAMLGG
                     DLPAVAAAGLKAGPSGGAAALDAFTLRVGRPVSPMLAQTATGVADALERHDGTTIFEA
                     KLDGARVQIHRAGDEVTVYTRSLDDVTARLPEVVEATLALPVTDLIADGEAIALRPDN
                     RPHRFQVTASRFGRSVDVAGALSAQRLSVFFFDILHCDGVDLLDAPTTERLAALDALV
                     PPPHRVDRLVTSDPSQAADFLNATLAAGHEGVMAKAPGAPYLAGRRGAGWLKVKPVHT
                     LDLVVLAVEWGSGRRRGKLSNIHLGARDPASGEFVMVGKTFKGMTDAMLDWQTARFSE
                     LAVPETAGAIDDYVVRVRPEQVVEIALDGVQRSSRYPGGLALRFARVVRYRDDKSPAE
                     ADTIDAVRALY"
     gene            4297..5064
                     /locus_tag="VFI55_00190"
     CDS             4297..5064
                     /locus_tag="VFI55_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7072489.1"
                     /translation="MEINGKKVVVIGGASGMGRATAELLAERGAQVAVLDREGSDGKS
                     VAEGLSGGAGAFYPVDVTDFAGTEQTLDTAVDKLGGLHVTVTTAGGGIAKRTLTKSGP
                     HDLESFQSVIDLNLVATFNISRIAAAQMAKNEPEDEERGVIINTASIAAFEGQIGQVA
                     YTAAKAGIAGMCLTMARDLGSMGIRVLAIAPSLFLTGLTSMVPDEMAASLTRDAAFPK
                     RMGRPIEYAKLVAAIVDNPMLNGQCLRLDAGQRFAPK"
     gene            5158..>5466
                     /locus_tag="VFI55_00195"
     CDS             5158..>5466
                     /locus_tag="VFI55_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7072490.1"
                     /translation="MGIALTDDHRELAEVARAFLTSHKARWAARSLLDTAEESRPEFW
                     QNLAELGWLGLHVDEEHGGSGYGLPELVVVIEELGRAVAPGPFVPTVIASAVIAKDGT
                     A"
BASE COUNT          982 a         1808 c         1833 g          843 t
ORIGIN      
        1 cggtgctgaa cagcacctcc tgcgtagtga tcagaacttc ggtggatgac tcctcaaccg
       61 agggtgtggc cgtggacgat tgaggcattt cctcgtcggg agggtcggtt gtgtcagcga
      121 atcccgttgg tggaagcgac atttcgtata cctccgttgc ggtgtggcat cggtggccgg
      181 gcgaatcggc cggtgatgca atcaccgtaa gagcgacgat ccgctctagg catgcgtagt
      241 cgactaccta tttctacgtg tgctcaacta ctcagttcga ggtagcgggc agcgctcaaa
      301 gctcttgagc accaaccaac ccgatggttg gcgccggcag cgcgtcccac tggcgttgca
      361 cttcgcgttc cctggccgtg gggatcatgc cggagggcgc gaaccgcttg gacggcgacg
      421 gatcttcctc ggtcaacggc atgccgccgc cgcgaattgc cctgagcgcg gcgacaagtc
      481 cgacgatcac cacgatgacg acgaccaccg cgaagaggat ggacagcgtg ccctggatga
      541 aggtattcct gatgacctca ttgatctgat ggacgttttt ggccgaaccg aacgcggttt
      601 tgcccgcgtg tttggcagcc agatattgcg aatgttgtgt ccagtagccg atcgcgggat
      661 ccgcggagaa gatcttctgc cacgacgcgg tcagcgtgac cgccagatcc cacagcagcg
      721 gagcgccggg gatccacgcc cacctcaaca ggcccttttt gatgacgacc acggtgatga
      781 cggtcagcgc tatggccgcg agcagctggt tggcgatgcc gaacagcggg aacagcgtgt
      841 tgatgccacc cagcggatcg gtcaccccca tcagcaggat gctgccccac gccgcggcca
      901 cggccaggct gcagatccac acgcccgggc gccagctggg gtttcgcagt ttggccagcg
      961 gaccgcccac gttgccgagc gagtcggaga gcatgaaacg cgcgacacgg gtgcccgcat
     1021 cgacggcggt caggatgaac agcgcctcga acatgatcgc gaagtggtac cagaacccct
     1081 tgagggccga gccgccgaaa actcgttgta gtacttccga catgcctacg gccagagtcg
     1141 gtgctccgcc ggtgcgcgac acgatggact tttcgcccac ggcggccgcg gcttgcttga
     1201 gctggtcggc ggtcgccggg gtccccgaca ggccgagccg gttgacgtag tcggcggcgc
     1261 tggccgcggt gccgccggtt tgcgcggctg gcgcgttgag cgtgaagtac aggtgctggt
     1321 cgaggatcga cgcgctgatc aacgccatca cggcgacgaa cgactcggtg agcatgccgc
     1381 cgtaaccgat cagccgcatc tggctttcct tctccaccag cttcggcgtc gtccccgacg
     1441 agatcagcgc gtgaaatccg gacagtgcgc cacacgcgat ggtgatgaac aggaacggaa
     1501 acagcgaacc ggcgaacacc ggtccgtcgc cgctggaggc gaatcgcgag atcgcgggtg
     1561 cggccatgag cgggcgcgca atgcagatgc cgatcgccag tagggcgatg gcgccgacct
     1621 tcatgaacgt ggacaggtaa tcgcgcgggg ccagcaacaa ccacaccggc agcaccgacg
     1681 ccacgaatcc gtagatgatg atcaaccacg acaccgtgac cggcgacagg ttcaaccagg
     1741 ttgtgcccca ggatgtttcg ccgacccaat ggccggacgc cacggcgagc atgagcagca
     1801 cgaagccgat gatcgatacc tcgcccactc gtccgggccg caggaaccgc agatagcagc
     1861 ccatgaagag ggcgatcggg atggtcatgg cgatggagaa cacgccccac gggctctggg
     1921 ccagtccccg gaccaccacc agcgcgagca cggcgatgat gatcaccatg atgacgaagg
     1981 tcccgatcag ggcggcggtg ccgccggtgg tgccgagctc gtcgcgcgcc atctgcccca
     2041 gggaacgacc tcgtcgcctc gtggagagcc acaacaccag gtagtcctgc acgccgccgg
     2101 cgaacaccgc gccgaggatg atccagatgc tgcagggcag atagcccatc tgggtggcca
     2161 gcaccggacc gacgagtggc ccggccccgg cgatggcggc gaaatggtgg ccgaacagca
     2221 cccgccggtc ggtcggcacg tagtcggtgc cgtcgtcgac gacttcggcc ggcgtcgcgc
     2281 ggtcgtcgcg cgggcgaacg atcttcattt cgatcaaccg ggcatagaac cggaatccga
     2341 ggatgtacgt gcagatcgcc gcgaccacaa accagaccgc gttcacggtc tcgccgcgag
     2401 cgaacgcgat gaccgcccag gcgatggccc cgatcagggc gatcacgccg aaggcgattt
     2461 tgtgccggat ggtgatgggg gagtggtcga tgatcgcgac gggcggtagg tctttctcgg
     2521 tgcggacgta gctgacgccg ttgtcgtgtt ctttaattgc cacggcgaaa accctacgcg
     2581 cgcaaatacg acctgaatat ggcaagcccg tttcgggcct ggtttcagta cagcgcccgc
     2641 acggcatcga tggtgtcggc ttcggccggg ctcttgtcgt cgcggtagcg caccacccgg
     2701 gcaaaccgca gcgccagtcc gccggggtag cgcgacgatc tttgcacgcc atccagcgcg
     2761 atctcgacca cctgctcggg gcgcactcgg acgacatagt cgtcgattgc ccccgcagtt
     2821 tcggggacgg cgagttcgct gaaccgggcc gtctgccagt ccagcatcgc atcggtcatg
     2881 cccttgaaag tctttcccac catgacgaat tcaccgctgg ccggatcacg ggcgcccagg
     2941 tggatgttgg acagtttgcc gcggcgccgc cccgaccccc actccacggc cagcaccacc
     3001 agatccagcg tgtgcacggg cttgaccttc aaccagcccg ccccgcgacg gcccgcgagg
     3061 tagggcgcgc cgggcgcctt ggccatcact ccttcgtggc cggcggccag ggtggcgttc
     3121 aggaagtcgg ccgcctgcga cggatcggac gtcacgagcc gatcgacgcg gtgcggcggc
     3181 ggcaccagcg cgtccagggc ggccaaccgc tcggtcgtcg gcgcgtcgag caggtcgacg
     3241 ccgtcgcaat gcaggatgtc gaagaagaac accgaaagcc gctgggccga aagggcccca
     3301 gccacatcaa cggatcggcc gaaccgcgac gcggtgacct ggaagcggtg cggccggttg
     3361 tcgggccgca gcgcgatcgc ctcaccatcg gcgatcaggt cggttaccgg cagcgccagc
     3421 gtcgcctcca ccacctcggg cagccgggcg gtgacgtcgt cgaggcttcg ggtgtagacg
     3481 gtgacctcgt cgcccgcccg gtggatctgc acccgcgcgc cgtccagctt cgcttcgaaa
     3541 atggttgtgc cgtcgtggcg ttcgagcgcg tcggcgacgc cggtggcggt ctgggccagc
     3601 atcgggctga ccggccggcc cacccgcagc gtgaaggcgt cgagcgccgc ggctccgccg
     3661 gacgggccgg ctttcaaacc ggccgccgcg accgccggca gatccccgcc caacatcgcg
     3721 gcacgttgta ccgcggcggc cgggagcccg gtggccctgg ccaccgcgtc ggccatgatc
     3781 ccggccagtg caccctgccg cagctccccg ccgagtaacc gcagcagaaa ggtctgctcg
     3841 gtctcggttg cggcgccgaa caatccggcg acgagttggg cgcgcctcgc ttgggccccc
     3901 ttgcccgaca cggctccgat ttccgagaac gtggcgtcga cgccgatgac ggtcagcacc
     3961 ggatgcccag gctcccgagg aggcggacgc gaccgcaacg acgcccagcc gacgccgatc
     4021 tggcgttgac gcagctcacc ggagagccac gacaccacga tcgcgaccgc ttcgggttcg
     4081 gctgcggcgc gccgcagcag gtcggcgatg tgcgaaacct tactcagccg cgacgaggtg
     4141 ccgcccaccc tgagcgatgt gcttgcgacg tcgagtagga gcacggttgt cagcttggca
     4201 cggctcgccg acaaccctgc cgggccgacg ggctagcgtg cgtagataac gttacagatt
     4261 gcagggattc tgataagtcc gacaaggagg gtccggatgg agatcaacgg gaagaaagtc
     4321 gtcgtcatcg gcggcgcgtc cgggatgggt cgtgccaccg cggagctgct ggccgaacgc
     4381 ggcgcgcagg tcgcggtgct cgatcgggag ggttccgacg gcaagagcgt cgccgagggc
     4441 ctcagtggcg gcgccggcgc gttctacccg gtggacgtca ccgacttcgc gggcaccgag
     4501 cagacattgg acaccgcggt cgacaagctg ggcggcctgc atgtcacggt cacgaccgcc
     4561 ggcggcggca tcgccaagcg gaccctgacc aagtccggtc cccacgacct cgaatccttc
     4621 caatccgtga tcgacctcaa cctcgtcgcc accttcaaca tcagccggat cgccgccgcg
     4681 cagatggcca agaacgagcc ggaggacgag gaacgcggag tcatcatcaa caccgcttcg
     4741 atcgcagcct tcgagggcca gatcggacag gtcgcctata ccgccgccaa ggccgggatc
     4801 gccggcatgt gcctgaccat ggctcgtgac ctggggtcga tgggaattcg tgtgctggca
     4861 atcgccccca gcctgttcct gaccggcctg acctcgatgg tgcccgacga gatggcggcc
     4921 agcctgaccc gcgacgcggc cttcccgaag cgcatgggcc ggccgatcga atacgcgaag
     4981 ctggtcgcgg ccatcgtgga caaccccatg ctcaacggcc aatgcctgcg gctggacgcc
     5041 ggacagcggt tcgcgcccaa ataaaaaccc ggtcggccgc gcttccgggc aataagtaca
     5101 ctctttagaa cggcccggca atgatgccgg ctgttcagcg gcgcgaggag ggtcctcatg
     5161 gggatcgcac tgacagacga ccatcgcgaa ctggccgagg tggctcgcgc gttcctgacc
     5221 tcacacaagg cgcggtgggc ggcgcgctcg ctgctcgata cggccgaaga atcccggccc
     5281 gaattctggc agaacctggc tgagctcggc tggctcggac tacatgtcga cgaagagcac
     5341 ggcggttcgg gctatgggtt gcccgaactc gtggtggtga tcgaagagct cggccgcgcg
     5401 gtggcgccgg gaccgttcgt gccgaccgtc atcgcatcgg cggtaatcgc caaagacggc
     5461 accgcc
//
LOCUS       DASSGQ010000007         2739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_61103, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000007 DASSGQ010000000
VERSION     DASSGQ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61103"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFI55_00200"
     CDS             <1..195
                     /locus_tag="VFI55_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072491.1"
                     /translation="RQPAIQETSSSSCSIGASRPGLPAHPCAVTHDQPYREGRRAACG
                     MHGGRDISATAIQEDPVGGN"
     gene            530..2137
                     /locus_tag="VFI55_00205"
     CDS             530..2137
                     /locus_tag="VFI55_00205"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HET7072492.1"
                     /translation="MRAVRGLIVAALSISVMALSPALAHADADPQPVDFTHNVTDAPA
                     PVPGAVFGTAPAVKTGTAICTTAAQLAANANTDCETTSAGPHNETSIAVNPTNPQNMI
                     GGANDYQIGLNPGGHVSETVLSRAHVTFDGGQTWSMFPVFSNSAYQATGDPAVAFDAA
                     GHAYYGTLGFRFVGPANAQNPDVLVSNSGDGGKTWNVVRVAAGSGNETSVGDLLDKEY
                     VAAWGNGNAIVTYGDFRLGQKGVTLSARIYSSVTHDGGNSWSKPVVISGSLDQAFVSV
                     PVVAADGRIYVSFLNTTDLATGRDDYEVVQVSPATGAPVAGPVKVATVIDGATDYPIA
                     LGRQTYQDSIFRTWAAGNITADPTNPAHLAVVWSDMRDSATPAPADPYTAKTNSDVIV
                     SQSFDRGATWSAPNALQRAGDQFMPWGAYDTSGHLRIGTFDRSVDPANHKYGYSLATE
                     TAAGTLAFTTAVISTATSDPTTSDLWFARTVNPAFPFATAFLGDYSNIAATADGHVVA
                     YWTDMRNDATFAGRTGHTEDAFFAKAS"
     gene            complement(2581..>2739)
                     /locus_tag="VFI55_00210"
     CDS             complement(2581..>2739)
                     /locus_tag="VFI55_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005572967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7072493.1"
                     /translation="VADRDELAAWVARLDELGIQHTGIRDEKEPMAYSTVVFRDPDNI
                     QLELIAFG"
BASE COUNT          465 a          955 c          883 g          436 t
ORIGIN      
        1 cggcagccgg ccatccagga gacgtcctcg agttcctgca gcatcggggc gtcccggccg
       61 gggctccccg ctcacccctg cgccgtgacc cacgatcagc cttaccgtga ggggcgacgg
      121 gccgcgtgcg gcatgcacgg cggacgcgat atcagcgcca cggcaatcca ggaagaccct
      181 gtcggcggca attgaggacg ttcacccaag ccgcggtgat gcataaacat acaggtcgtc
      241 tgtcactggc accggcgacc atggcatttt ggaccggtgt tcacggctgc gcagcgctgg
      301 caggagacca gccggcccgg ctgctcaaag ccatgtgccg tttttgccgg tggccggagg
      361 gagccggata ttagcccgtg cggtgaggcg gccctgtgca cggttgattc cggacctgtt
      421 atcaacccgt gactagcggt gcggacattg ctgtggcaga ttctgtcaga gcgagcggga
      481 ttctggggtg gggtcatggg tgtccgcgcc ggaggtaagg gagggtgtga tgagagcggt
      541 tcgaggcctg atcgtggcgg ctctatcgat atcggtgatg gccttgagcc cggcgctggc
      601 ccatgcggac gccgatccgc agccggtgga cttcacgcat aacgtcacgg acgcgccagc
      661 gcccgtcccg ggcgccgtgt tcggcaccgc gccggcggtc aagaccggca cggcgatctg
      721 cacgaccgcg gcgcagctcg ccgccaacgc caacaccgac tgcgagacga catcggctgg
      781 cccgcacaac gagacgtcga tcgcggtcaa cccgacgaac ccgcagaaca tgatcggcgg
      841 ggcgaacgac taccagatcg gcctgaatcc gggcggtcac gtgagcgaga ccgtgctttc
      901 gcgcgcgcac gtgacgttcg acggcggcca gacctggtcg atgttcccgg ttttctccaa
      961 ctccgcctac caggcgaccg gggacccggc cgtagcgttc gacgccgccg gccacgccta
     1021 ttacggcacg ttgggcttcc gcttcgtcgg tccggccaac gcccagaatc ccgacgtcct
     1081 ggtctccaac tctggtgacg gcggcaagac ctggaacgtg gtgcgggtcg ccgcgggcag
     1141 cggtaacgag accagcgtcg gcgacctcct ggacaaggag tacgtcgcgg cgtggggtaa
     1201 cggcaacgcc atcgtcacct atggcgactt ccggcttggc cagaagggcg tgaccctctc
     1261 ggcgcggatc tacagctcgg tcacccacga cggcggcaac agctggtcca agcccgtggt
     1321 gatctcggga agcctcgatc aggcgttcgt gtccgtcccg gtggtagcgg cggacggccg
     1381 catctatgtc tccttcttga acacgaccga cctggccact ggtcgcgacg attacgaagt
     1441 agtccaggta tccccggcga ccggcgcgcc cgtggcgggc ccggtcaagg tcgcgaccgt
     1501 catcgacggc gccaccgact acccgatcgc tctcggccgg cagacctacc aggacagcat
     1561 cttccgcacc tgggcggcgg gcaacatcac cgccgatccg accaacccgg cccacctggc
     1621 cgtcgtctgg tccgacatgc gcgacagcgc gacacccgcc cccgccgacc cgtacacggc
     1681 caagacgaac tccgacgtga tcgtgagcca gtcattcgat cgcggagcga cgtggtcggc
     1741 gcccaacgcg ctgcagcgcg cgggcgacca gttcatgccg tggggcgcat acgacacctc
     1801 gggccacctg cgcatcggca cgttcgaccg ttcggtcgat cccgccaacc acaagtacgg
     1861 ttattcgctc gccaccgaaa cggcagctgg aacgctggcg ttcaccacgg ccgtcatctc
     1921 gacggcaacc tccgatccca ccaccagcga tctgtggttc gcccgcaccg tgaaccccgc
     1981 gttcccgttc gcgaccgctt tcctcggcga ctacagcaac atcgccgcta ccgcggacgg
     2041 tcacgtggtc gcctactgga ccgacatgcg caacgacgcg accttcgccg ggcgcaccgg
     2101 gcacaccgag gatgccttct tcgcgaaagc cagctgaccg gcgaccttca gtaaggaggc
     2161 aagcgggcgg cggcgggcac atgccgccgc ccgcttgccc tcgcctatac gccggtggcg
     2221 tgccgccctg caccgatctt ctatacgccg ccgcccagcg cgccccagcg ctgacacggt
     2281 cgcgcaatat cggtacgctc cctggcggcg cacggcaacg aggcggcgga gccctgcgag
     2341 atcaacagga ccacggataa gtatgccaaa taacgcatat gtatccgaat gaccaacgcc
     2401 cgctattcgt catgtgagtg aggtgtcgca accactcgcg gcgtgaacgt ttcagccctg
     2461 ctggccgggg cctagaattg ggccaccagg tcggtgggta cccggccgcg gtctttttac
     2521 ctcgatgccg tgcgccctgg cccacccgcg gacctcgcgg agcgagcgtc gctggtttcg
     2581 tcagccgaac gcgatgagct ccagctggat gttgtcgggg tcccggaaca ccaccgtgga
     2641 gtaggccatg ggttccttct cgtccctgat gccggtgtgc tggatgccga gctcatcgag
     2701 cctggccacc caggccgcca gctcatcgcg gtcagcgac
//
LOCUS       DASSGQ010000008         6128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_91871, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000008 DASSGQ010000000
VERSION     DASSGQ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91871"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            644..940
                     /locus_tag="VFI55_00215"
     CDS             644..940
                     /locus_tag="VFI55_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072494.1"
                     /translation="MDNERRYSHAVCTGIEVRKVNGFPNRSKASTSFGERQNLSMRTG
                     MRRFTRLINAFYRQVETLAHVASLHYMNYDFARPHQTLHQGQRRDRTTPEMVQG"
     gene            1325..1734
                     /locus_tag="VFI55_00220"
                     /pseudo
     CDS             1325..1734
                     /locus_tag="VFI55_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014350546.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            1727..2758
                     /locus_tag="VFI55_00225"
     CDS             1727..2758
                     /locus_tag="VFI55_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET7072495.1"
                     /translation="MPDPSVVGIEPEAITRWFDQLGVAFTGPLRFERIGLGQSNLTYL
                     VRDTDDHRWVLRRPPLGQLLASAHDVAREARIMAALAATDVPVPQILGVAQNPEIADV
                     PLVLMEFVDGQVVDNMAIAESLAPQRRWQIGVSLPTMLAKIHAVDIDAVGLSDLASHK
                     PYAHRQLKRWAGQWERSKTRELPELDDLTRRLVAAAPEQRELSLVHGDFHLRNAIVSG
                     GTSEVIGVLDWELSTLGDPLADMGTMLAYWPEPGEEMGDFEASTLDGFPDRAELARIY
                     LDQTGRDPHALKYWHVLGSWKIAIIAEGVMRRAMDTQENKAASGTPTVKWIDALVDAA
                     REVADAEGI"
     gene            2772..3968
                     /locus_tag="VFI55_00230"
     CDS             2772..3968
                     /locus_tag="VFI55_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005252933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7072496.1"
                     /translation="MTFDEQAIIRDLGTRVRQLLDTHDPGHTDPQEFLAARFDAGLAW
                     VQIPEGHGGLAVPQSFQEHVNAALAAAGAPEPAVAKNGIGFGMAAPTIAAFGTEEQKR
                     KFLRPLYIGEHIYCQLFSEPGAGSDFASLATRAVRDGSGNGDWIVNGQKVWTSGAQNA
                     QMAILVARTDPNVPKHAGLTYFLCDMSDPGVEVRPLRQITGEAEFNEVFLTDVRVPDA
                     NRLGAEGQGWQVALTTLNNERVAIGSGVPSAMIDNVTAAWRAQPELRNPAMLDELVRL
                     WVESEAARLTGERLRQQLASGQPGPEGSAMKLALARLTQAISGFEIQLHGEAGLRYDD
                     WTLRRPETVDFIGRGPGYRYLRAKGNSIEGGTSEILRNVIAERVLGLPPEHRVDKDIA
                     WKDLTR"
     gene            3965..5071
                     /locus_tag="VFI55_00235"
     CDS             3965..5071
                     /locus_tag="VFI55_00235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014350550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7072497.1"
                     /translation="MSTANLLYTDTEDALRDSVRRLFADRRKPEEVVALYDHPDADVT
                     DIWKTLAAELGLAGLLVPESLGGAGASTREAAVVMEEIGRAVAPVPFLSSAVLATVAL
                     LGAGETQTLPLLAGGSTTGALTIPLSTPPGAFAATVRVDASGLTGRVSSVADANTADV
                     LVVPVAGPDGLELHTVSRAAAGVQITPVLSLDMTRPIADVEFSAVASARADNGSAEGA
                     VAEALLCGAVLLASEQVGVAQWCLEATLGYVKQRKQFGRAVGSFQALKHRLADLWLEV
                     SSAGATARYAADTYARGGAETPIAAAVAQSYCSDVAVHAAEECIQQHGGIGMTWEYPA
                     HLYLKRAKSDQLALGTPYRHRARLAELIDLPTSA"
     gene            complement(5129..5917)
                     /locus_tag="VFI55_00240"
     CDS             complement(5129..5917)
                     /locus_tag="VFI55_00240"
                     /inference="COORDINATES: protein motif:HMM:NF005095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7072498.1"
                     /translation="MPTATTTLQIDLTGKRALVTGGTRGIGKGITDRLRAAGATVVVA
                     ARSAPRTPSPQVITADIATADGSATIAERTLAILGGVDIVVHNVGGSQVSPGSVTNLT
                     DDDWNQAFQINLFGAVRLDRALVPSMISRRSGVIIHITSVQRRLPMPGSAPYAAAKAA
                     LANYSKALSDELAPNGIRVNSVCPGFIETEAAAAFVSEHAAALQISVDAARAQVIKSI
                     GGIPLGRTGDPTDVGDLVVFLASDHARYLTGSEFRIDGGSTRTV"
BASE COUNT         1068 a         1944 c         2089 g         1027 t
ORIGIN      
        1 gattccgtca acggatcacc gcgatcggcc agcggttcgc gaccggtaac cgccacccga
       61 gccatggctg atgatccaac tcgcgttccc agggtggcgc cgcccggctg ttgctgggat
      121 cggtagccgg agcgaacgca agaatttcgc aaatggtcgg cgacaacgta gggccaggtc
      181 cgacagcaac gaaaggggcc accatgaaca ccactcacac ccggacacgg ctcattgccg
      241 cggcgctgct atcggggttt ttgggtctgg ccggattcga gctggccgca ggtaccgctc
      301 aggccgctcc atgttccaac taccctggcc aaccggcgag ttgtgccccg cctgaggggc
      361 cccaggggtg ctatgactca gggatctgca gccaagaatg gtgtccaaat atgggcccaa
      421 tgcggaggat gcccaactgg gacatgagtg tctgccacac ctaattactt tgcgcctaac
      481 tctggtctcc ccgcggagat aatccaagga gagcccccgg ggttgctggt tcctcgtcag
      541 gtcgtgcccc ggtggctatg ccctgggcaa ggcgcgggcg gttgttgatc gtcgctgaat
      601 gtgacgacga cgtcgtccag atagcacgga atcgcccggc ggggtggata acgagcgccg
      661 gtacagccac gccgtctgca ccgggatcga ggtccggaag gtcaacggct tccccaaccg
      721 gtctaaggcc agcacgtcat ttggggagcg ccagaacctc agcatgcgga cgggaatgcg
      781 ccggttcacc cgcctcatca acgccttcta caggcaggtg gagaccctcg cgcacgtggc
      841 gtccctgcac tacatgaact acgacttcgc gcgcccccac cagacgctcc accaaggaca
      901 acggcgcgac aggacgacgc ccgagatggt gcagggatag aacatcgcgt ctggacccac
      961 cgcgacatcg ccgcgctcag tcaaggaacg cacgcgcccc agcagctaag cgcgaatcaa
     1021 actgacccat cgtctcccgg aatttgaact tgacgtcgaa ttcaggtatt gggtataagg
     1081 aacgagacgc gttttgcgtc aagccccggc cgtggcgggt gtcctgccgg cgcacggttg
     1141 ccgggcgagt gagcgtcgtc gagcagagct aggagcacgc atgcccatcg gtgagaccgt
     1201 tcgcggtccg gtcgataccg ggccgacgac gcgcaccgcg gtgctgatcg atttcggggg
     1261 cgtactcacc actgccggcg ccaacgtcgt cctcacctca cgcaaggagg acgccgccga
     1321 cgaggccacc aagacgtgga tgggcgagca cggcggttcg gtgatcaaca ccgcatcgat
     1381 cggcgggctg ggccacgaga cgggcctcgg cgtgtacaac accacgaagg cagcgctgat
     1441 ctacctgacc aagcaactgg cgcttgagct ttcgccgaag atacgggtca actcggtcgc
     1501 gccgggcgtg gcacgcaccc ggcaggccga ggcgctgtgg aaggaccacg agcaacaggt
     1561 cgcggcgtgc ccgcgctgga ccggatcggt gaacccgacg acgtcgccgc tgccgtcgtg
     1621 ttcctcgcct ccgcgaccgc ggcgtggatc accggcgaga caatggtgat cgacggcggc
     1681 caacggctcg gcgacgcccg cccgtaccag ttcggagcaa tggctgatgc ctgacccgtc
     1741 ggttgtcggg atcgagccgg aagcaataac acgctggttc gaccaactgg gagtcgcgtt
     1801 caccgggccg ttgcgattcg aaaggatcgg cctcggccag tccaacctga cctacctggt
     1861 gcgcgacacg gacgatcacc gctgggtgct gcgtcgaccc ccgctcggtc aactgctggc
     1921 ctcggcacac gacgtcgcac gcgaggcgcg gatcatggcc gcgctcgctg ctaccgacgt
     1981 ccccgttccc caaatcctcg gcgtggcaca aaatccggaa atcgccgacg taccactggt
     2041 gctcatggag ttcgtcgacg gacaagtcgt cgacaacatg gcgattgccg aatcgcttgc
     2101 cccgcaacgg cgctggcaga tcggggtgtc tttgccgacg atgctggcca agattcacgc
     2161 ggtggacatc gacgcggtgg gcctgtccga cctggccagc cacaagccgt atgcgcatcg
     2221 tcagctcaaa cggtgggccg gccagtggga gcggtcgaag acccgcgaac tgcctgagct
     2281 cgacgacctg acccgccgcc tggtcgccgc agcccctgag caacgtgagc tgtcattggt
     2341 gcacggcgac ttccacctgc gcaacgccat cgtgtctggc ggcactagtg aggtgatcgg
     2401 cgtgctggac tgggagttgt ccacgctcgg agatccgttg gcggacatgg gcacaatgct
     2461 cgcctactgg ccagagcctg gcgaggaaat gggcgacttc gaggcgtcga cgctggacgg
     2521 cttcccagac cgggccgagc tcgcgcgcat ctacctggac caaaccgggc gggatccgca
     2581 cgcgctgaag tactggcacg tactgggatc gtggaagatc gccatcatcg ccgagggcgt
     2641 catgcgccgc gcgatggaca cccaggagaa caaggcggcc tccgggactc cgacggtcaa
     2701 gtggatcgac gcattggtcg acgccgcccg cgaggtcgcc gacgcggaag gtatttagcg
     2761 aggacgaaaa catgacattt gacgaacagg cgattatccg cgatctcggc acccgagttc
     2821 ggcagctgct ggacacccac gatccggggc acaccgatcc gcaggaattt cttgccgcga
     2881 gattcgacgc cggcctggca tgggtgcaaa ttccggaggg acacggcgga ctcgctgtgc
     2941 cgcaaagctt tcaggagcat gtgaacgccg ccctggccgc ggccggcgcg cccgagcccg
     3001 cggtggccaa gaacggcatt ggcttcggca tggccgcgcc gactatcgcc gcattcggaa
     3061 ccgaggaaca gaaacgaaag tttctgcggc ccttgtatat cggtgagcat atttactgcc
     3121 agttgttcag cgaaccgggt gcgggttcgg atttcgcctc gctcgccacc cgcgcggtgc
     3181 gcgacggctc gggaaatggg gactggattg tcaacgggca gaaggtgtgg acctccggcg
     3241 cccagaatgc gcagatggcg atcctggtcg cgcgaaccga cccgaacgtg ccgaagcacg
     3301 ccggattgac gtacttcctg tgcgacatga gcgatccggg tgtggaggtc cggccgctgc
     3361 gccagatcac cggggaggcc gagttcaacg aggtgttcct caccgacgtc cgggtacctg
     3421 acgccaaccg tctcggcgcc gaaggccagg gctggcaggt cgccctcacc acactgaaca
     3481 acgagcgcgt cgccatcgga tccggcgtcc caagcgcgat gatcgacaac gtcaccgccg
     3541 cctggcgcgc gcaacccgaa ctgcgcaacc cggcgatgct cgacgagctg gtgcggctgt
     3601 gggtcgagtc ggaggcagca cggctaacgg gtgaacgcct gcgtcagcaa ttggcttccg
     3661 gccaaccggg tcccgagggc tccgcgatga aactcgccct ggctcggttg acacaagcga
     3721 tctccggctt cgagatacaa ctgcacggcg aggccggcct gcggtacgac gactggacat
     3781 tgcggcggcc ggagacggtc gacttcatcg gacgcgggcc cggttaccga tacctgcgcg
     3841 ccaaaggcaa ctcgatcgag ggcggaacct cggagatcct gcgcaacgtg atcgccgaac
     3901 gagtgctcgg tctgccgccg gaacatcgtg tcgacaagga cattgcctgg aaggacttga
     3961 cccgatgagc accgccaacc tgctctacac cgacaccgag gatgcgctgc gcgacagcgt
     4021 ccggcggctg ttcgccgatc gccgcaagcc ggaagaggtc gtcgcccttt acgaccaccc
     4081 cgatgcggac gtcaccgaca tctggaagac gctcgccgcc gaactcggac tcgccggact
     4141 ccttgtgccc gagtcgctcg gcggtgcggg cgccagcacc cgcgaggcgg ccgtggtcat
     4201 ggaggagatt gggcgcgcgg tcgcccccgt cccatttctg tccagtgcgg tgctggccac
     4261 cgtcgccctg ctcggggcgg gcgagacgca gacgctgccg ttgttggccg gcggatcgac
     4321 gacgggtgcg ctcacgattc cgctgtccac cccgccgggt gccttcgccg cgaccgtgcg
     4381 ggtcgatgcg tcgggcttga ccgggcgagt cagcagtgtc gcggacgcca acaccgccga
     4441 cgtgctcgtg gtgccggttg ccggacccga cggcctcgag ttgcacacgg tctcccgcgc
     4501 agcggcgggc gtgcagatca ccccggtgct gtcgctggac atgacgagac cgattgccga
     4561 cgttgaattt tcggccgtcg cttccgcgcg cgccgacaac gggtccgccg agggcgcggt
     4621 ggccgaggcg ctattgtgcg gcgcggtgct gctggcttcc gagcaggtcg gcgtggcgca
     4681 gtggtgtctg gaggcgacac tgggctacgt caagcaacgc aagcagttcg gccgggccgt
     4741 cggctcgttc caggcgctca agcatcgcct ggccgacctg tggttggaag tcagttccgc
     4801 gggcgctacc gcacgctacg cggccgacac ctacgcccgc ggcggtgcgg agaccccgat
     4861 cgcggccgca gtggcgcagt catactgcag cgatgtcgcc gtgcacgccg ccgaagagtg
     4921 catccagcag cacggcggca tcggcatgac gtgggagtat ccggcgcact tgtatctcaa
     4981 gcgggccaag agcgaccagc tcgcactcgg caccccttac cgccaccgtg ctcggctcgc
     5041 cgaactcatc gacttgccga cgtcagcgtg agggccagga cgcggcagca cgcgtctggg
     5101 gttcgagcgg tcagccggtt ggtgtggttt agacggtgcg ggtgctgccg ccgtctatgc
     5161 ggaattcgga tccagtcagg tagcgggcgt ggtcggatgc caggaaaacg acgaggtctc
     5221 cgacgtcggt cggatctccg gttctgccaa gggggatgcc accgatggat ttgatgacct
     5281 gtgcgcgtgc ggcatcgacg ctgatctgta gtgctgcggc gtgctcgctg acaaatgccg
     5341 cggcagcttc ggtctcgatg aatccggggc agacactgtt gacacggata ccgttgggcg
     5401 ccaattcatc cgatagcgct ttgctgtaat tggccagggc ggccttggcg gcggcgtagg
     5461 gtgcgctacc tggcatcggc aggcggcgtt gcactgaggt gatgtggatg atgacgccgc
     5521 tccggcgact gatcatcgag gggacgagcg ctcgatcgag tcgtacggct ccgaacaggt
     5581 tgatctggaa ggcttgattc cagtcgtcgt cggtcaggtt tgtgacgctt ccggggctta
     5641 cctgggaacc gccgacgttg tgcacgacga tgtccacgcc gccgagaatg gccagggtgc
     5701 gttcggcgat ggttgccgag ccgtcggcgg tggcgatgtc ggcggtgatc acctgaggcg
     5761 acggcgtgcg gggggcgctg cgcgcggcga cgacaacagt cgcgccggca gcacgcaatc
     5821 gatcggtgat gcctttgccg atgcctcggg tgcctccggt gacgagggct cgtttgccgg
     5881 tgaggtctat ctgcagcgtg gtggtggcag taggcatggt gtccctccgc gggtcggtgc
     5941 tcgccggatt ctcgtgtgct cagcgtgccg gttgttcagt gggggtatcg atgatcggcc
     6001 aggcgatttc ggtgcgccat tgggtggagt cagtggtgtg gctgtggtct cggtggtagt
     6061 actcgcgtag ttgccagccg acggctagtt cgtgttcggc gatgtaggaa cccaagtggg
     6121 cgtacgcc
//
LOCUS       DASSGQ010000009         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_942445,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000009 DASSGQ010000000
VERSION     DASSGQ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942445"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2104
                     /gene="uvrB"
                     /locus_tag="VFI55_00245"
     CDS             <1..2104
                     /gene="uvrB"
                     /locus_tag="VFI55_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019731486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HET7072499.1"
                     /translation="RRVAPFEVITDMVPAGDQPAAIAELERRILAGERDVVLLGATGT
                     GKSATTAWLIERLQRPTLVMAPNKTLAAQLANELREMLPHNAVEYFVSYYDYYQPEAY
                     IAQTDTYIEKDSSINDDVERLRHSATSSLLSRRDVVVVASVSCIYGLGTPQSYLDRSV
                     ELQVGSEVPRDGLLRLLVDVQYSRNDLSFTRGSFRVRGDTVEIIPSYEELAVRIEFFG
                     DEIEALYYLHPLTGEVIRQVDSLRIFPATHYVAGPERMAHAISTIEEELAERLAEFEG
                     QGKLLEAQRLRMRTNYDIEMMRQVGFCSGIENYSRHIDGRSAGSPPATLLDYFPEDFL
                     LVIDESHVTVPQIGGMYEGDMSRKRNLVEYGFRLPSAVDNRPLTWEEFADRIGQTVYL
                     SATPGPYELSQASGEFVEQVIRPTGLVDPQVVVKPTKGQIDDLIGEIRKRTDADERVL
                     VTTLTKKMAEDLTDYLLEMGIRVRYLHSEVDTLRRVELLRQLRLGDYDVLVGINLLRE
                     GLDLPEVSLVSILDADKEGFLRSSRSLIQTIGRAARNVSGEVHMYADTMTASMKEAID
                     ETERRRAKQVAYNQAKGIDPQPLRKKIADILDQVYREAADTQAGESVLIGGSGRNASR
                     GRRAQGEPGRAVSAGVFEGRDVAAMPRAELADLIKDLTAQMMAAARDLQFELAARFRD
                     EIADLKKELRGMDAAGLK"
     gene            2101..>2469
                     /locus_tag="VFI55_00250"
     CDS             2101..>2469
                     /locus_tag="VFI55_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7072500.1"
                     /translation="MTHTTGITGSWRELLGAKYLGTAVVLAGGVALYATNEFLTISLL
                     PNTVAEIGGSRLYAWVTTLYLVGSVVAATMVNPMLVRIGARPSYLMGLALFGASSLAC
                     GAAPNMEILIAARAAQGVAGG"
BASE COUNT          431 a          804 c          837 g          397 t
ORIGIN      
        1 gcgccgcgtc gccccgttcg aggtcatcac tgacatggtg ccggccggcg accagccggc
       61 cgcgatcgcc gagctggagc ggcgcatctt ggccggggag cgcgacgtcg ttttgctcgg
      121 cgcgaccgga accggaaagt cggcgaccac cgcgtggctc atcgagcgcc tgcagcggcc
      181 caccctggtg atggcgccca acaagacgct ggccgctcag ctggccaacg agctacgaga
      241 aatgctgccg cacaacgctg ttgagtactt cgtctcctac tacgactact accagccaga
      301 ggcatacatc gcgcagaccg acacctacat cgaaaaggac agctccatca acgacgacgt
      361 ggagcggctg cggcactcgg cgacgtcgtc gctgctgtcg cgtcgcgatg tggtggtggt
      421 ggcctcggtg tcatgcatct acggcctggg cacaccgcag tcctacctgg accgctccgt
      481 cgaattgcag gtcggctccg aggtgcctcg ggatgggttg ctgcggttgc tggtcgacgt
      541 gcagtacagc cgcaacgacc tgtccttcac ccgcggctcg ttccgggtgc gcggcgacac
      601 ggtcgagatc atcccgtcct acgaagaatt ggcggttcgc atcgaattct tcggcgacga
      661 gatcgaggcg ctttactacc tgcacccgct gaccggcgag gtgattcggc aggtcgattc
      721 gctgcggatt tttcccgcca cgcactatgt ggccggcccg gagcggatgg cacatgcgat
      781 ctccaccatc gaggaggaac tcgccgagcg gctcgccgaa ttcgagggcc agggtaagct
      841 cttggaggcc cagcggctgc ggatgcggac caactacgac atcgagatga tgcggcaggt
      901 cgggttctgc tcgggcatcg agaactactc gcgccacata gatgggaggt cggcagggtc
      961 ccctcccgcg acgctgctgg actacttccc ggaggatttc ctgctcgtca tcgacgagtc
     1021 gcacgtcacg gtcccgcaga tcggcggcat gtacgagggc gacatgtcgc gcaagcgcaa
     1081 cctcgtcgag tacgggttcc ggttgccgtc ggcggtggac aacaggccgc tgacctggga
     1141 ggagttcgcg gaccggatcg gacagacggt gtacctgtcc gcgaccccag ggccgtatga
     1201 gctcagccag gccagtggtg agttcgtcga gcaggtgatc cggccgaccg gcctggtgga
     1261 cccgcaagtg gtggtcaagc cgaccaaggg gcagatcgac gacctgatcg gcgagatccg
     1321 caagcggacc gacgccgacg agcgcgtgct ggtcaccacc ctgaccaaga agatggccga
     1381 agacctcacc gactacctgc tggagatggg cattcgggtg cgctacctgc actccgaggt
     1441 cgacacgcta cgccgggtgg aactgctgcg gcagctgcgg ctcggcgact acgacgtgct
     1501 ggtcgggatc aacctgctgc gcgagggcct ggacctgccc gaggtgtccc tggtgtccat
     1561 cctcgacgcc gacaaggaag ggtttttgcg gtcgtcgcgc agcctgatcc agaccatcgg
     1621 ccgcgccgcc cgcaatgtct ccggtgaagt gcacatgtac gccgacacga tgaccgcctc
     1681 gatgaaagag gccatcgacg agaccgaacg gcggcgggcg aagcaggtcg cctacaacca
     1741 ggccaagggc atcgacccgc agccgctgcg caagaagatc gccgacatcc tcgaccaggt
     1801 ctaccgcgag gccgccgaca ctcaagccgg cgagtccgtc ttaatcggcg ggtcgggacg
     1861 taacgcctcc cgcggccggc gcgcccaggg tgagcccggc cgcgcggtga gcgccggtgt
     1921 gttcgagggc cgtgacgtcg ccgcgatgcc acgcgccgaa ctggccgatc tgatcaaaga
     1981 cctcaccgcg cagatgatgg ccgccgcgcg tgacttgcag ttcgaactgg cggccaggtt
     2041 ccgggacgag atcgccgacc tcaaaaagga attgcgcggg atggatgccg ccggcctgaa
     2101 gtgacgcaca ccacgggcat caccggcagc tggcgggagc tgctgggcgc caaatacctg
     2161 gggactgcgg tcgtgctggc cggcggtgtc gcgctgtatg ccaccaacga attcctgacc
     2221 atcagcctgc tgccgaacac ggtcgccgaa atcggcggca gccgcctcta cgcatgggtg
     2281 accaccctgt acctggtcgg gtcggtggta gcggcgacca tggtcaatcc gatgctggtg
     2341 cgcatcgggg cgcgtccgtc gtacctgatg gggttggcct tgttcggcgc ctccagcctg
     2401 gcatgcgggg cggcccccaa catggagatc ctgatcgcgg cccgcgccgc gcagggtgtg
     2461 gccggcggc
//
LOCUS       DASSGQ010000010        27940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1064007,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000010 DASSGQ010000000
VERSION     DASSGQ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 27940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27940
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064007"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFI55_00255"
     CDS             <1..252
                     /locus_tag="VFI55_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011594087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HET7072501.1"
                     /translation="ATACAPPRPVAVDDGGIGAERAATTAAGGQYADLTDLFCAAERC
                     PVIVGNTLVFRDDNHVTTEYAQLLTPVVGALADRALSGG"
     gene            complement(496..1191)
                     /locus_tag="VFI55_00260"
     CDS             complement(496..1191)
                     /locus_tag="VFI55_00260"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL lipase"
                     /protein_id="HET7072502.1"
                     /translation="MSRLATFIVSLAFLLGVVCQPMAHPARVRAYGALTLDSRLNHIA
                     VIGDSYTTGTDEGGRGPNSWTALAWHALAQRGVPIAADVAAEGKAGYVAPGDHGSIFE
                     DLTSRAVKPDDALVVFFGSRNDQGVDPALLAGRAHDTFDLARLLAPTARFLVIGPPWP
                     TADVPQPVLQIRDVLNAMARAAGAAFVDPIGDRWFVDRPDLIGPDGVHPNDAGHQYLA
                     NNIAPLIRTQLSR"
     gene            complement(1358..2599)
                     /locus_tag="VFI55_00265"
     CDS             complement(1358..2599)
                     /locus_tag="VFI55_00265"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET7072503.1"
                     /translation="MTGEIRGEIKALTGLRIIAAVWVVLFHFRPMLNDASPDLRDALA
                     PVLNCGAQGVDLFFILSGFVLTWNYLDRMGRSWSTRATLHFLWLRLARVWPVYLVTLH
                     LAALWVIFTMHVGHIPSPDAGQLTAISYVRQVLLVQLWFVPFFDDSSWDGPAWSISAE
                     WLAYLLFGALVLVILRMTQATRARSLMLLAFAASLPPVLMLLASGHFYTPWSWLPRIV
                     TQFIAGALACAAVRRLRLTDRGRHVAGFISLLLLAAMVGVLYWFKAHPISGVVENDSG
                     GVVDMLFVPLVITLAIGLGSLPRLLSTRLLVYGGQISFCLYMVHELVHTAWGWAVEQF
                     ELTPWHSDSPWKWNVIGLLAIAVGLSSLMYHFVEEPARRWMRRMVEVRPARTKIDVAE
                     PVGANVHPIDGAREAVSARAV"
     gene            3001..3462
                     /locus_tag="VFI55_00270"
     CDS             3001..3462
                     /locus_tag="VFI55_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7072504.1"
                     /translation="MITAIAGSASNARQGNGTFDCDGAQIRAHCRHLATVVTIRGDID
                     AVNVDRVRECVRRFILGSNFVVLDVSGVSYFASGISLLHRVDEDCRAAGVGWTLVASP
                     TVVELVGDSFDRDEYGAMFPIALSAHDALHNLADAIVNRRELVLPLIAKSA"
     gene            complement(3443..3703)
                     /locus_tag="VFI55_00275"
     CDS             complement(3443..3703)
                     /locus_tag="VFI55_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072505.1"
                     /translation="MIARYRALVELVLAFAALAGAAVSWAHARHTVHVAPVADGQPFT
                     TSLVYDPQLLLLTLVLLTTAGVLEVIGVARLWRERTATPTSL"
     gene            complement(3700..4245)
                     /locus_tag="VFI55_00280"
     CDS             complement(3700..4245)
                     /locus_tag="VFI55_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3093 domain-containing protein"
                     /protein_id="HET7072506.1"
                     /translation="MTTARDVKPKPLFYEPGASWYWVLTGPAAAVSMIMIEIWSGAGA
                     QLPIPAMFLVMVSAFVGLQVKAARVHVSVELTEDTLRQGTETILVREIVRVYPEAEHN
                     EASGKELAKWQSARTLGELVGVPRGRIGIGLKLTEGRTAQAWARRPRLLRAALTPLVQ
                     ERVEPEAGLTDDDRDDDGPLQ"
     gene            complement(4287..5267)
                     /gene="hemB"
                     /locus_tag="VFI55_00285"
     CDS             complement(4287..5267)
                     /gene="hemB"
                     /locus_tag="VFI55_00285"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HET7072507.1"
                     /translation="MPYPRQRPRRLRSTPALRRLVAQTSLEPRHLVLPMFVADGIDDP
                     RPIASMPGVLQHTRDSLRSAAAAAVAAGVGGLMLFGVPREQDKDPAGSAGTDPDGILN
                     VALRDLAKDLGDATVLMADTCLDEFTDHGHCGVLDARGRVDNDATLARYVKLAVAQAE
                     SGAHVVGPSGMMDGQVAAIRDGLDAAGHTEVVILAYAAKFASAFYGPFREAVSSSLSG
                     DRRTYQQEPGNIREALREVQLDLDEGADIVMVKPALGYLDVVAAAASMSPVPVAAYQV
                     SGEYAMICAAAANGWIDARAAALESLTSIRRAGADIVLTYWAAEAADWLS"
     gene            complement(5271..6938)
                     /locus_tag="VFI55_00290"
     CDS             complement(5271..6938)
                     /locus_tag="VFI55_00290"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HET7072508.1"
                     /translation="MTTRGRKSRPGRITFVGSGPGDPGLLTTRAATVLANAALVFTDP
                     DVPQPVLALIGKDLPPVSGPVPAGPVAGSDDGSTDGDSGRAAPAVISGGPDIRPALGE
                     PAEVAKTLTAEARSGADVVRLVAGDPLTVDSVITEVNAVARSHLHIEIVPGLAATSAV
                     PTYAGLPLGSSHTVADVRDPHVDWEALAAAPGPLILQATASHLADAARALIDHELADT
                     TPCVVTAQGTTCQQRSVETTLQGLTDPAVLGGTDPAGPMTGPLVVTIGKTVASRAKLN
                     WWESRALYGWTVLVPRTKDQAGEMSERLTSYGALPIEVPTIAVEPPRSPAQMERAVKG
                     LVDGRFQWVVFTSTNAVRAVWEKFGEFGLDARAFSGVKIACVGESTADRVRAFGISPE
                     LVPAGEQSSLGLLDDFPPYDSIFDPVNRVLLPRADIATETLAEGLRERGWEIEDVTAY
                     RTVRAAPPPAATREMIKTGGFDAVCFTSSSTVRNLVGIAGKPHARTIIACIGPKTAET
                     AAEFGLRVDVQPETAAVGPLVDALAEHAARLRAEGALPPPRKKSRRR"
     gene            complement(6962..7909)
                     /gene="hemC"
                     /locus_tag="VFI55_00295"
     CDS             complement(6962..7909)
                     /gene="hemC"
                     /locus_tag="VFI55_00295"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET7072509.1"
                     /translation="MIRIGTRGSLLATTQAGVVRDALIANGHPAELVTITTAGDQSSA
                     PIETLGVGVFTTALREAIEDGRVDAAVHSHKDLPTAADPRFTVAAIPRRNDPRDAVVA
                     RDGLVLGELPPGSLVGTSSPRRAAQLRALGLGLEIRPLRGNLDTRLNRVSSGDLDAIV
                     VARAGLARLGRLDDVTETLEAVQMLPAPAQGALAVECRAGDSRLAAVLAELDDADTRA
                     AVTAERALLAELEAGCSAPVGAIAEVVESIDEDGRVFEELSLRGCVAALDGSDVIRAS
                     GVGTPGRARELGISVAAELFELGARELMGTARQDPARGN"
     gene            complement(7923..9311)
                     /locus_tag="VFI55_00300"
     CDS             complement(7923..9311)
                     /locus_tag="VFI55_00300"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014712431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HET7072510.1"
                     /translation="MSVLLFGVSHRSAPVSVLEQLSIDESDQVKIVDRMLQSPLVTEA
                     MVLSTCNRVEVYAVVDAFHGGLAVVGQVLSEHSGMSMGELTKYAYVRYSEAAVEHLFA
                     VASGLDSAVIGEQQVLGQVRRAYAAAETNRTVGRVLHELAQRALSVGKRVHSETAIDA
                     AGASVVSVALGMAERKLGGLAGKTAVVIGAGAMGALSSAHLTRAGIERVQVLNRSLSR
                     GQRLARKTRESGVRAEALTLDQLADVLPDADVVVSCTGAVSPVVSLADVHHALAAARR
                     DEATHPLVICDLGMPRDVDPAVAGLPGVWVVDVERVQDEPSAHAAAADVDAARNIVAS
                     EVAAYLAGQRMAEVTPTVTALRQRAADVVEAELLRLDNRLPGLESTQREEVARTVRRV
                     VDKLLHAPTVRIKQLASAPGGDSYAEALRELFELDQTAVDAVAAGELPVVSSGFDAGP
                     TRGAGGPQPSGD"
     gene            complement(9496..9765)
                     /locus_tag="VFI55_00305"
     CDS             complement(9496..9765)
                     /locus_tag="VFI55_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HET7072511.1"
                     /translation="MTQPTRRPQVELLTRDGCSICARVHARLAELAGELGFELSTTDV
                     DAAASAGDPGLRAEFGDRLPVVLLDGREHSYWEVDEPRLRADLAR"
     gene            9837..10148
                     /locus_tag="VFI55_00310"
     CDS             9837..10148
                     /locus_tag="VFI55_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HET7072512.1"
                     /translation="MAADGTLRADPEAMRGFTEAVCASAEDLRNRLAALDDQVGQLLG
                     GWRGASGNAYGAAWELWHRGAGEVEMGLSMLARLVAEAGGMYSANEAVSAQELREVDR
                     G"
     gene            10141..10431
                     /locus_tag="VFI55_00315"
     CDS             10141..10431
                     /locus_tag="VFI55_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014712434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HET7072513.1"
                     /translation="MAEPFRVDTDALADAVQRMMEFQRCAEQMLTEIDSLVTNLHTTW
                     TGRGAAAHAEAHRHWARGEAMMREALAHLRTSGAIAHRNYTGAMSTNVGMWS"
     gene            10598..11509
                     /locus_tag="VFI55_00320"
                     /pseudo
     CDS             10598..11509
                     /locus_tag="VFI55_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
     gene            11554..12066
                     /locus_tag="VFI55_00325"
     CDS             11554..12066
                     /locus_tag="VFI55_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HET7072514.1"
                     /translation="MTVHPDAEGLIGSHYRAPDYFEVGREKIREFALSVKDEHPAHFS
                     EIDAAAAGYPGVVAPLTFLAVAGRRVQLEIFTKFNIPINIARVFHRDQKFRFHRPIVA
                     NDKLFFDTYLDSVLESHGTVIAEIRSEVTDADGNPVVTSTVTMLGEAAQQDAGAEETV
                     AAIAAISAGK"
     gene            12077..13000
                     /locus_tag="VFI55_00330"
     CDS             12077..13000
                     /locus_tag="VFI55_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane mycolic acid synthase family
                     methyltransferase"
                     /protein_id="HET7072515.1"
                     /translation="MTSQGENHATQLKPPVEAVRSHYDKSNEFFKLWLDPSMTYSCGY
                     FDENPDPQHLTKTLEEAQYAKRKLALDKLGLKPGMTLLDIGSGWGSTMRHAVAEYDVD
                     VIGLTLSENQYAHCVAEFDKMDSPRRKEVRIQGWEEFDEPIDRIVSLGAFEHFADGAG
                     DAGYERYATFFKKYYDLLPDDGRMLLHSIVVPSREEGNAMGLKVNMTLLRFISFILKE
                     IYPGGKLPQVDLVDKYSTGAGFNIERHHFIGKNYVPTLTAWGDALEAHKEEAIALKGQ
                     ETYDIYLKYLRGCSDLFRDGYTNVCQFTMVK"
     gene            complement(13144..14211)
                     /locus_tag="VFI55_00335"
     CDS             complement(13144..14211)
                     /locus_tag="VFI55_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7072516.1"
                     /translation="MAGETRANVIPLHTNRARVAARRRADQRAESSRQHPSLLSDPNG
                     RASAEQIAAVVREIDEHRRAAGATPPAEAPLNELAQRVAAVAGFLRQRLAGDYTVDEF
                     GFDPHFNSAIVRPLLRFFFRSWFRVEVSGIENLPSDGAALLVANHAGVLPFDGLMLSV
                     AVHDEHPAHRDLRLLAADMVFDLPVIGEAARKAGHTMACTTDAHRLLAAGELTAVFPE
                     GYKGLGKRFEDRYRLQRFGRGGFVSAALRTKAPIVPCSIIGSEEIYPMLTDVKLLARL
                     FGLPYFPVTPLFPLAGPAGLVPLPSKWRIAFGEPIYTNDHATSDAEDPMVTFELTDQV
                     RETIQQTLYRLLAGRRNIFLG"
     gene            complement(14217..15344)
                     /locus_tag="VFI55_00340"
     CDS             complement(14217..15344)
                     /locus_tag="VFI55_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7072517.1"
                     /translation="MDQSNGDGGGTGGTATNTVHYPKIVLVTGACRFLGGYLTARLVQ
                     DPLIKGVIAVDAIAPSKDMLRRMGRAEFVRADIRNPFIAKVIRNGDVDTVVHAAAASY
                     APRSGGSAALKELNVMGAMQLFAACQKAPTVRRVVLKSTSEVYGSTAHDPVMFTEDSS
                     SRRPFRDGFAKDSLDIEGYVRGLGRRRPDIAVTILRLANMIGPAMDTTLSRYLAGPVV
                     PTLFGRDARLQLLHEQDALGALERAAMAGKAGTFNIGADGIIMLSQAIRRAGRIPLPV
                     PGFGVWALDSLRRANRYADVTRDQFNYLSYGRVMDTTRMRSELGYQPKWTTAEAFDDY
                     VRGRGLTPIIDPHRVRSLEGRAIALAQRWGSRNPIPRGGVR"
     gene            complement(15406..15507)
                     /locus_tag="VFI55_00345"
     CDS             complement(15406..15507)
                     /locus_tag="VFI55_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HET7072518.1"
                     /translation="MGSVIKKRRKRMSKKKHRKLLRRTRVQRRKLGK"
     gene            complement(15694..15951)
                     /locus_tag="VFI55_00350"
     CDS             complement(15694..15951)
                     /locus_tag="VFI55_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/environmental response
                     transcriptional regulator"
                     /protein_id="HET7072519.1"
                     /translation="MTSTNGPSARDSAGKPRDTSSGDGQQARTQFLTVAEVAALMRVS
                     KMTVYRLVHNGELPAVRVGRSFRVHAKAVHDMLETSYFDAG"
     gene            complement(16092..16967)
                     /gene="proC"
                     /locus_tag="VFI55_00355"
     CDS             complement(16092..16967)
                     /gene="proC"
                     /locus_tag="VFI55_00355"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET7072520.1"
                     /translation="MARIAIIGGGSIGEALLSGLLRSGQQVKDLVVAERVPERAKYLA
                     DTYSVLSTSVTEAVDNATFVVVAVKPADVDSVMGELARAAAAAENDSAEQVFVTVAAG
                     IAISYFEARLPAGTSVVRAMPNAAALVGAGVTALAKGRFVTPPQLEGVSALFDSVGGV
                     LTVPESQMDAVTALSGSGPAYFFLLVEALVDAGVAAGLSREVATDLTTQTMAGSAAML
                     LARMDSDRRGVEGEEAGRWADTTAAQLRATVTSPGGTTAAGLRELERGGLRAAVDAAV
                     QAAKRRSEQLRITSE"
     gene            complement(17004..17807)
                     /locus_tag="VFI55_00360"
     CDS             complement(17004..17807)
                     /locus_tag="VFI55_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET7072521.1"
                     /translation="MTLCEVEPGTYEGELDKHWTIGPKVHGGAMLALCANAARTACAG
                     GSSDGLGPVLEPVSVSASFLWAPDPGPMLLTTLIRKRGRRISVVDVALIQGDRTAVHA
                     VVNLGEPEHGTVPLLSANPVLDLMPPEPPGDVAPIGPGHPLAGLVHLGEGCDVRPLLS
                     TLAPSAEGRPPVIQMWARPRGVAPDPLFALMCGDLSAPVTFAVDRTGWAPTIQLTAFL
                     RGLPADGWLRVVCTCVEIGHDWFDEDHTVVDSSGRIVVQSRQLAMVPAR"
     gene            complement(17834..18676)
                     /locus_tag="VFI55_00365"
     CDS             complement(17834..18676)
                     /locus_tag="VFI55_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET7072522.1"
                     /translation="MRPAIKVGLSTASVYPLRAEAAFEYAARLGYDGVELMVWSESVS
                     QDVEAVKNLSERYRVPVLSVHAPCLLISQRVWGSNPILKLERSVRAAEELGAQTVVVH
                     PPFRWQRRYAEGFSEQVLELEASSDVMIAVENMFPFRADRFFGAGQSRERMRRRGGGP
                     GPAISAFAPSYDPLDGNHAHYTLDLSHTATAGTDSLEMAERMGEGLVHLHLCDGSGLP
                     ADEHLVPGRGTQPTAEVCQLLAAGRFAGHVILEVSTSSARSAYEREAMLVESLQFART
                     HLMR"
     gene            complement(18714..19730)
                     /locus_tag="VFI55_00370"
     CDS             complement(18714..19730)
                     /locus_tag="VFI55_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072523.1"
                     /translation="MTGPHSEKEEPGSGPISVAELLAKNGTVGSPAVTRRRRRRRGDS
                     DAVTVAELTGEIPVIRDHHDETDHDEAGHGKAERGANGAARVVESAPTEPTPEPVTEE
                     PPKKAYWSQPEPRWPKSPPQPPRSGPERSAYPRPLPYAEQSGDRTSQSGAEDMSPDPV
                     DNYADVPVDVMDSEVRDADLAAEDSAFVRSYLQSDSMLFGGQTLADEVARRRGGQQEA
                     AELDAERKVDEDRPDTKAKGAGDARVHGVRERLDALWRGSVIVFQSILAVAFGAGLFV
                     AFDQLWRWNSIVALVLSVLVILGLVVGVRVVRKTEDIASTLIAVAVGALITLGPLALS
                     LQSG"
     gene            complement(19727..20743)
                     /locus_tag="VFI55_00375"
     CDS             complement(19727..20743)
                     /locus_tag="VFI55_00375"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HET7072524.1"
                     /translation="MRLGVLDVGSNTVHLLVVDAHRGGHPTPMSSTKATLRLAEATDS
                     SGKITKRGADKLISTIDEFAKIAVSSGCAELMAFATSAVRDAENSDDVLARVRKETGV
                     ELEVLRGVDESRLTFLAVRRWYGWSAGRIINLDIGGGSLELSSGVDEDPEVALSLPLG
                     AGRLTREWLADDPPGRRRVAMLRDWLDAELAEAGVTVLEAGGPDLAVATSKTFRSLAR
                     LTGAAPSAAGPRVKRTLTANGLRQLIAFISRMTTADRAELEGVSAERAPQIVAGALVA
                     EASMRTLSIEEVDICPWALREGLILRKLDSEADGSALVESPVRDAGGQVIDRNSNRSR
                     GDKR"
     gene            21031..21816
                     /locus_tag="VFI55_00380"
     CDS             21031..21816
                     /locus_tag="VFI55_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072525.1"
                     /translation="MANSHPQPGQEVELDFAREWVEFYDPDNPEHLIAADLTWLLSRW
                     TCVFGTPACRGTVAGRPDDGCCSHGAFLSDDDDRARLDDAVKTLTDADWQFRDKGLGR
                     KGYLELDEHDGQPQYRTRKYRDACIFLNRPGFAAGAGCALHTKALKLGVAPLTMKPDV
                     CWQLPIRRSQEWVTRPDGVEILKTTVTEYDRRGWGSGGADLHWYCTGDPAAHVGAKQV
                     FESMADELTELLGAKAYAELAAMCKRRSKLGLIAVHPATRLAE"
     gene            complement(22205..22984)
                     /locus_tag="VFI55_00385"
                     /pseudo
     CDS             complement(22205..22984)
                     /locus_tag="VFI55_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725447.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23388..24788
                     /locus_tag="VFI55_00390"
     CDS             23388..24788
                     /locus_tag="VFI55_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7072526.1"
                     /translation="MLGRLPPWLRPRYWGIAARSAFVSATVVLVALVVAGGGLVVVLD
                     RSLTASLDAAAAARLQDVAAGLQTDTAGELDARLLATDQRIVGVQVIDAAGAVIARSP
                     STPVAALAPIEVAGSNPRVGIPPANALTDRDMRIAAQTVSSAGGHYTVLVAVGTEAVE
                     STVRTIGFGLALAAPIVIAVAAAATYLLVGRSMRSVEAIRARVAEISVSDLAGRVPVP
                     EHRDEISALAVTMNAMLSRIESGQAAQRRFVADASHEMRSPLATVISALEVGVAHPQL
                     LNPQLASTTLLPEAHRMQSLVEDLLLLARADERGLALRHDDVDLDDLVAAETARLQRE
                     TALAVHIDVTPVRVQGDASAMSRAVRNLLDNAARHAYSRIHVTAHTSDGHAEFTVSDD
                     GPGISAPDRLRVFDRFVRLDTGRARSGGGTGLGLAIVAEIVAAHGGSVRIAEPAGGGT
                     VVIVQLPVGKSPDSRR"
     gene            complement(24749..25438)
                     /locus_tag="VFI55_00395"
     CDS             complement(24749..25438)
                     /locus_tag="VFI55_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7072527.1"
                     /translation="MKVLVVEDEPHLVATLAMGLRAEGFVVVTATTGIDGLWQASEHS
                     FDVVVLDIMLPRMNGYEVVRQLRAKEIWTPVLMLTAKDGEYDQTDALDLGADDYLTKP
                     FSFIVLVARLRALMRRGAPQRPVVLTAGSLSLDPVRRAVHRSSTPISLTPREYALLEY
                     LMRNKDAVVTKTNILHNVWDHHYGGADNVVEVYVGYLRRKIDAPFGTNTIDTIRGVGY
                     RLESGDFPTGN"
     gene            complement(25587..25769)
                     /locus_tag="VFI55_00400"
     CDS             complement(25587..25769)
                     /locus_tag="VFI55_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072528.1"
                     /translation="MNIRKRVAIALAGSAIVLGAMGAAAVAVGAPTHVPLVAPASGPD
                     APDLPEPGDSPDGPDD"
     gene            26638..27900
                     /locus_tag="VFI55_00405"
     CDS             26638..27900
                     /locus_tag="VFI55_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072529.1"
                     /translation="MTETTKPEAVRAQRALLSKVPEVTVWFWLIKILCTTVGESFADW
                     INTTLGFGLTMTALAFAALLAAVLGWQLRLNRYVVLVYWLTVVVLSVTGTLYTDILTD
                     KLGVPLALSTSVFAAALAVIYAVWYARERTLSIHSIVTLPRELFYWVAVLVTFALGTA
                     AGDWTLQLTGWGPGTAVLLPTGLIISIVMGWRLGANPVLSFWLAYTLTRPLGANLGDW
                     FASPSSSHGLGLGTAATSALFLTAILATVVYLTRTRSDVIEDYDRTHTPIVTTNPGRE
                     RIMLSYYAAVAMATGALLVWAAGQHHATATTNDDHEAAAAPVTAQPLSGQATAHFPPA
                     EIAKFRTISQDTLDKLRAGDQAGATARINDLETAWDGAQSTLQPLDENAWQVLDRQID
                     NALKALRASRPDPATERQTLTTLLTALQ"
BASE COUNT         4763 a         9580 c         9148 g         4449 t
ORIGIN      
        1 gccaccgcct gtgcgccacc gcggccggtc gcggtcgacg acggcggtat cggcgccgag
       61 cgggcggcga ccaccgccgc aggcggacag tacgccgacc tgaccgacct gttctgcgcc
      121 gcagagcgtt gcccggtcat cgtcggcaac accctggtgt ttcgggacga caaccacgtg
      181 acaaccgagt atgcgcagct gctgacaccc gtcgtcggtg cgctggcaga ccgcgcgctg
      241 agcgggggct gagacgggga tgaacttcaa cccgcacgcg aacgtcggat caagtgaagg
      301 cttttggaga tctgggagag gtttgagatg actgtgctgc tgctgttgat ctgcatagcg
      361 gtcgtcgctc ctgtcgccgg cattggcctg atgcacatgc aggcgcggct ggagcagtgg
      421 gcctacgagc gccacgcgga agactgaagc gtcaaacgac acgtttggcg gcgatttgct
      481 atatccgccg ggcgctcacc gggacaattg cgtgcggatg agcggtgcga tgttgttcgc
      541 caggtactgg tgacccgcgt cgttagggtg cacaccatcc gggccgatca ggtcgggtct
      601 gtcgacgaac cagcggtcgc cgatcggatc gacgaacgcc gcgcccgcgg cccgggccat
      661 ggcgttgaga acgtcgcgaa tctgtagcac cggctggggt acatcggcgg ttggccacgg
      721 cggtccgatc accaggaacc tggcggtcgg cgccagcagg cgcgccagat cgaacgtgtc
      781 gtgggccctt ccggccagca gcgcgggatc gacgccctgg tcgttgcggg agccgaagaa
      841 caccaccagc gcgtcgtcgg gcttgaccgc ccgcgaggtc aggtcctcga agatgctgcc
      901 gtggtcgccg ggtgcgacat agccggcctt cccctccgcc gccacatcgg cggcgatcgg
      961 aacgcctcgc tgagcgagcg cgtgccaggc cagggcggtc cacgaattcg ggccacggcc
     1021 gccctcatcg gtgccggtgg tgtaggagtc gccgatcacc gcgatgtggt tcagccggga
     1081 atcgagggtc agcgcgccgt aggcccgcac ccgcgccggg tgcgccatgg gttgacacac
     1141 gacgcccaac agaaacgcga ggctcacgat gaacgtagcc aggcgactca ccaaaacctc
     1201 cccggggaac ctgcgtcgtc gtgcgccgca tccaaccgga atcgggcgcg gaaacgaccg
     1261 tgaactcggc gtgaacccgc ggcatcgcgg ccgcccgcaa ccgacatggt attcgacaag
     1321 ttctgggcgg cgcgcgattc gcgcaccgag gtaatcgtca caccgcgcgg gccgaaaccg
     1381 cctcccgtgc accgtcgatc gggtgaacgt tggcgcccac cggctcggcg acgtcgattt
     1441 tcgtgcgcgc gggtcgcacc tcaaccatcc ggcgcatcca gcgacgggcg ggctcttcga
     1501 cgaagtggta catcaggctc gacagcccca cggcgatcgc gagtaggccg atgacgttcc
     1561 atttccacgg ggagtcggag tgccacggtg tgagctcgaa ttgctcgacc gcccatcccc
     1621 aggccgtatg caccagctcg tgcaccatgt acaggcaaaa cgagatctga ccgccgtaca
     1681 ccagcaaccg tgtcgacagc agcctcggca ggctgcccaa gccgatcgcc agcgtgatca
     1741 ccagcgggac gaacagcatg tcgaccacgc cgccgctgtc gttctccacg accccggata
     1801 tcggatgcgc cttgaaccag taaaggacgc ccaccatggc agccaggagg agcagcgaga
     1861 tgaaccccgc gacgtggcgg ccacgatcgg tcagccgcag cctgcgcacg gcggcgcagg
     1921 ccagcgctcc cgcgatgaac tgggtcacga tgcgcggcag ccagctccac ggtgtgtaga
     1981 agtggccgct ggccagcaac atcagcaccg gcggcaacga cgcggcgaac gccagcaaca
     2041 tcaggctgcg cgccctcgtc gcctgcgtca tccgcaagat caccagcacg agcgcgccga
     2101 aaagcaggta ggccagccat tccgcactga tcgaccaggc cggcccatcc cagctggagt
     2161 catcgaagaa cggcacgaac cacaactgca ccagcaggac ttggcgcacg tagctgatcg
     2221 cggtgagctg acccgcgtcc ggcgacggga tgtgaccgac gtgcatcgtg aagatcaccc
     2281 acagggcggc aaggtgcagg gtgaccaggt acaccggcca caccctggcc agccgcagcc
     2341 acaggaagtg cagggtggcg cgcgtcgacc aggaccggcc catccggtcg aggtagttcc
     2401 aggtcagcac gaatccactg agaatgaaga acaggtcgac gccttgcgcg ccgcagttga
     2461 gcaccggcgc gagggcgtcg cgcaaatcgg gcgaggcgtc gttgagcatc ggccggaagt
     2521 gaaacagcac gacccatacc gcggcgatga tccggagtcc ggtcagggcc ttgatctcgc
     2581 ccctgatctc gccggtcatc tcttcggtgc gcacgatgct ctttccgtcc ggattgtgct
     2641 gtctcgttcg ccgtctcgtc cagctgaccg gctacccgcc acaccacaag ccacgtcaca
     2701 agccccccga cgagcgaccg gcagcctccg aagcctaaca gcgggactcc ggcgatgacg
     2761 gcaaagggcg atcgcccggc gcgcggtcac gcccgcagcg gaccggcgtc gacgttgccg
     2821 tcagcgtcgg tcggcacccc gaaattcgct tcttagcgaa gagttagacg cggctgtcgg
     2881 tccccttaga ttttgctggc accgttagag gcgtgttcgg tcggcgttgc gtcggcccac
     2941 tcgaccgcat caggttcagc ggccgcggcc acacggaaca caaggggaat ggggctggtc
     3001 atgattaccg cgatcgctgg gtcggcttcg aacgctcgtc aggggaacgg cactttcgac
     3061 tgcgatggcg ctcagatccg cgcgcactgt cgtcacctgg cgacggtggt caccatccgg
     3121 ggcgacatcg acgccgtcaa cgtcgaccgg gtccgcgagt gcgtccggcg tttcatcctc
     3181 gggagcaact tcgtggtgct cgacgtcagc ggcgtgagct acttcgcctc cggcatatcg
     3241 ctcctgcaca gggtcgacga ggactgccgc gccgccgggg tggggtggac cctggtcgcg
     3301 agcccgacgg tcgtcgagct ggtgggcgac agctttgacc gcgacgagta cggggccatg
     3361 ttcccgatcg cgctctcggc acacgacgcg ctccacaacc tggccgacgc catcgtcaac
     3421 cgtcgtgagc tcgtgctgcc gctcatagcg aagtcggcgt agcggtccgc tcgcgccaca
     3481 gcctggcgac gccgatcacc tccagcactc cggcggtcgt cagcaatacc agggtcaaca
     3541 gcagcagctg agggtcatat accaacgacg tggtgaacgg ctgcccgtcc gcgacaggcg
     3601 cgacatgcac cgtgtgacgt gcatgcgccc agctcacggc cgcgccggcc agcgcggcaa
     3661 aggccagcac gagttcgacc agagcccggt accgggcgat cactgcaggg gcccgtcgtc
     3721 gtcgcgatcg tcatcggtga ggccggcttc gggctcgaca cgctcctgga ccaacggggt
     3781 cagcgccgcc cgaagcaacc gcggacgacg cgcccaggcc tgggcagtac gaccctctgt
     3841 gagcttgagg ccgatgccga tgcggccgcg cggtaccccg accagttcgc ccagcgttcg
     3901 cgccgactgc cacttcgcca gctcctttcc ggacgcctcg ttatgctcgg cctccggata
     3961 gaccctgacg atctcacgca ccaaaatggt ttcggtgccc tggcgcaggg tgtcttcggt
     4021 gagctcgacc gagacgtgga cgcgggccgc cttcacttgc aaccccacga acgccgacac
     4081 catcaccaga aacatcgccg ggataggcag ctgcgcgccg gcgccgctcc atatctcgat
     4141 catgatcatg gacaccgccg cggccgggcc cgtcagcacc caataccagc tggcacccgg
     4201 ttcgtagaac agtggcttgg gtttgacgtc ccgcgctgtg gtcattggaa gcctcatccc
     4261 ctgattgtca cccgccgccc gcgcagtcag gaaagccaat ccgccgcctc cgcggcccag
     4321 taagtgagca cgatatcggc tcccgcgcgg cggatgctgg tcaacgattc cagcgcggcg
     4381 gcgcgcgcgt cgatccagcc gttcgcggcc gcggcgcaaa tcatcgcgta ctcccccgat
     4441 acctggtagg cggccaccgg caccggcgac atcgaggccg ccgcggccac gacgtcaagg
     4501 taacccaacg cgggtttgac catcacgatg tcggcgccct cgtcgagatc cagctggacc
     4561 tcgcgcagcg cctcccggat gttgcccggc tcctgttgat aggtgcgccg gtccccggac
     4621 aggctggagc tcaccgcctc gcggaatggg ccgtagaacg ccgaggcgaa tttcgcggca
     4681 taggccagga tcaccacctc ggtgtggccc gcggcgtcca gtccgtcgcg aatcgctgcc
     4741 acctgaccgt ccatcatccc gctcggcccg accacatggg cacccgattc cgcttgcgcc
     4801 acagccagtt tcacgtatcg agccaaggtg gcgtcgttgt ccacccggcc cctggcatcc
     4861 agcaccccgc agtgcccatg gtcggtgaac tcgtccaggc aggtgtcggc catcagcacg
     4921 gtggcgtcac cgagatcctt ggctaggtca cgcagcgcga cattgagaat gccgtcgggg
     4981 tcggtgccgg ccgagccggc tgggtccttg tcctgttcgc ggggcacgcc gaacagcatc
     5041 agcccgccta ccccggcggc gacggcagcc gcggcggcgc tgcgcagcga atcgcgggtg
     5101 tgctgcagca cgcccggcat cgaggcgatc ggccgcgggt cgtcgatgcc gtcggcgacg
     5161 aacatcggga gcaccaaatg ccttggctcc aaggatgttt gcgccaccaa acgacgcaaa
     5221 gcgggcgttg agcggagccg gcgcggccgc tgccgcgggt agggcattag ctagcgcctg
     5281 cggctcttct tacgcggcgg tggcagcgcg ccctcggcgc gcaaccgcgc ggcgtgctcg
     5341 gccagggcgt cgaccagcgg gccgactgcc gctgtctcgg gctggacgtc gacccgcagg
     5401 ccgaactcgg ccgcggtctc ggccgtcttg gggccgatgc aggcaatgat ggtccgcgcg
     5461 tgcggcttgc cggcgatgcc gaccaggttt cgcaccgtgg agctggaggt gaagcagacc
     5521 gcgtcgaagc cgcccgtctt gatcatttcg cgggtggccg ccggtggcgg cgcggcgcgc
     5581 accgtccggt aggcggtgac gtcctcgatc tcccagccac gctcgcgcag accctcggca
     5641 agcgtctcgg tggcgatgtc ggcccgcggc agcagcacgc ggttcaccgg gtcgaaaatg
     5701 ctgtcgtagg gcgggaagtc gtccagcaga ccgagcgagg actgctcgcc ggccggcacc
     5761 aactcggggc tgatcccgaa ggcgcggacc cggtcggccg tcgactcacc gacgcaggcg
     5821 atcttgacgc cggagaacgc gcgggcgtcc agaccgaact cgccgaactt ctcccacacc
     5881 gcgcgcaccg cgttggtgga ggtgaacacc acccactgga agcggccgtc gaccaggccc
     5941 ttgacggccc gctccatctg cgcggggctg cgcggcggct cgacggcgat ggtcggcacc
     6001 tcgatcggca gcgcaccgta ggacgtcagc cgctcgctca tctcgccggc ctggtccttg
     6061 gtgcgcggca ccaacacggt ccagccgtac agcgcacggc tttcccacca gttcagcttc
     6121 gcccggctgg ccaccgtctt accgatggtc accaccagcg gaccggtcat cggaccggcc
     6181 ggatcggtgc cgcccaacac ggccggatcg gtcagccctt gcagcgtggt ttccaccgaa
     6241 cgctgctggc aggtggtgcc ctgggcggtc accacgcagg gcgtggtatc ggccagctcg
     6301 tgatcgatca gggcgcgggc cgcatcggcc aaatgcgacg cggtggcctg caggatcagc
     6361 gggccggggg ccgcggccaa agcttcccaa tccacatgtg ggtcgcgcac gtcggcgacc
     6421 gtgtgcgagg aacccagcgg cagccccgcg taggtgggga cggcgctggt ggcggccagg
     6481 cccggcacga tctcgatgtg caggtggcta cgggcgacgg cgttcacctc ggtgatgacc
     6541 gagtcgaccg tcagcgggtc accggccacc agccgcacca cgtcggcgcc ggagcgggcc
     6601 tcggcggtca gcgtcttggc gacttcggcg ggctcaccca gggccggccg gatgtccgga
     6661 ccgccggaga tcaccgcggg cgcggcccgg cccgagtcgc cgtcggtgct gccgtcgtcg
     6721 ctgccggcaa cgggacccgc cggcaccggt ccggagaccg gtggcaggtc tttgccgatc
     6781 agcgccagca cgggctgggg gacgtcgggg tcggtgaaca ccagggcggc gttggccagc
     6841 accgtggcag cccgcgtggt cagcaatccc gggtcgcccg gaccggagcc cacgaacgtg
     6901 atccggcccg gcctcgactt acgccctcgc gtcgtcattg tcgctcccaa gtgattctca
     6961 ttcagtttcc tcgcgcgggg tcttgccgcg ccgtccccat caactcccgg gcgccgagtt
     7021 cgaacagctc cgcggcgacc gagatcccca gctctcgtgc ccggccggga gtaccgacgc
     7081 cggacgcgcg gatcacgtcg gacccgtcca gcgccgccac acagccacgc agcgacagct
     7141 cttcgaagac gcggccgtcc tcatcgatgg actcgaccac ttctgcgatc gcgcccaccg
     7201 gtgcggaaca tccggcctcc agttcggcca gcagagctcg ctccgcggtg accgccgcgc
     7261 gtgtgtcggc gtcatccagc tctgccagca ccgcggcgag ccggctgtca ccggcgcggc
     7321 attcgacggc gagcgcgcct tgtgccggtg ctggcaacat ctgcaccgcc tccagcgtct
     7381 cggtcacatc gtcgaggcgg cccagccggg ccagaccggc ccgggccacc acgatcgcgt
     7441 ccagatcacc actgcttacc ctgttcaacc tggtatctag gttgcctcgt agggggcgga
     7501 tttccaaacc gagacccaat gctctaagct gtgcggcccg ccgcggggac gacgtgccca
     7561 ccagcgaacc cggtggcaac tcccccagca ccaacccgtc gcgggccacc accgcgtcgc
     7621 gggggtcgtt ccgccggggt atcgccgcca ccgtgaacct tggatcggcc gcggttggca
     7681 aatccttgtg cgagtgcacc gcggcatcca cccggccgtc ttcgatggcc tcacgcagcg
     7741 cggtggtgaa gacgcccacg cccagcgttt cgatgggcgc cgacgactgg tcacctgcgg
     7801 tggtgatggt caccagctcc gccgggtggc cgttggcgat cagagcgtct ctgacgacgc
     7861 cggcctgggt ggtggccaac agactgcccc gcgtgcctat ccggatcacg tgtcccaatc
     7921 agctaatcgc ccgacggctg tggaccgccc gcgccgcggg tagggcccgc gtcgaatccg
     7981 cttgacacca ctggcaattc gcctgcggcg acggcgtcga cggcggtttg gtcgagttcg
     8041 aagagttcgc gaagggcctc cgcgtagctg tcgccgccgg gggcgctggc gagttgtttg
     8101 atccgcacgg tgggcgcgtg taacagcttg tcgaccaccc gccgcacggt gcgggccacc
     8161 tcctcgcgct gggtgctctc caggccgggc aaccggttgt cgaggcgcag cagctccgct
     8221 tcgaccacgt cggcggcccg ctggcgcaac gcggtcaccg tgggggtgac ctcggccatt
     8281 cgctgtcccg ccagataggc ggcgacctcg gacgccacga tgttgcgggc ggcgtcgacg
     8341 tcggcggccg cggcgtgggc cgacggttcg tcttgcacgc gctcgacgtc gacgacccag
     8401 acgccgggca gcccggcgac cgccggatcg acgtcgcgcg gcatgcccag gtcgcagatc
     8461 accagcgggt gggtcgcctc gtctcgacgg gccgcggcca gcgcgtggtg cacgtcggcc
     8521 agcgaaacca ccgggctgac cgcgcccgtg cagctgacca cgacgtcggc gtcgggcagc
     8581 acatcggcca gctggtcgag ggtcaacgcc tcggcccgaa ctccggattc gcgggtcttg
     8641 cgggccagcc gctgtccccg ggacagggac cggttgagca cttggacgcg ttcgatgccg
     8701 gcgcgggtca ggtgcgccga cgacagggcg cccatcgctc cggccccgat gaccacggcg
     8761 gtcttgcccg ccagcccacc cagcttgcgt tcggccatcc ccagggcgac cgacaccacc
     8821 gaagcgccgg cggcgtcgat cgccgtctcg gagtgcaccc gcttgcccac cgacagtgcc
     8881 cgctgggcca gctcgtgcag cacccggccg acggtgcgat tggtctccgc ggcggcatac
     8941 gcgcggcgca cctggccgag gacctgttgc tcgccgatca ccgccgaatc cagaccgctg
     9001 gcaaccgcga acaagtgctc tacggccgcc tcgctgtagc ggacgtaggc gtacttggtc
     9061 agctcaccca tcgacatgcc ggagtgttcc gacagcacct gcccgaccac cgccaacccg
     9121 ccgtggaacg cgtcgacgac ggcgtagacc tcgacccggt tgcacgtcga cagcaccatc
     9181 gcctcggtca ccagcggtga ctgcaacatg cggtcgacga tcttgacctg atcggattcg
     9241 tcgatgctga gttgttccag gacggatacc ggcgcactgc ggtgcgaaac cccgaagagc
     9301 aggacgctca cggcagcatc acctggccag ctcccttgct cgctccagta actgaactgg
     9361 tgtccacggt aaacgtttat caggtgggct accaaatgat cggccgcgat tttccggtgg
     9421 cctgaccggg agcaacgtgg gcggctcggt tcgacgcctg gacggccggt caacggcccg
     9481 attcggcccg ccctgtcatc gagcgaggtc agcgcgcagc cgtggctcgt cgacctccca
     9541 gtagctgtgc tcgcggccat ccagcaggac caccggcagg cgatctccga actcggcccg
     9601 caaccccggg tcgcccgccg acgccgcagc gtcgacgtcg gttgtggaca gctcgaagcc
     9661 cagctcaccg gccagttccg ccagccgcgc gtggacccgt gcgcaaatgc tgcagccgtc
     9721 gcgggtcaat agctccacct gcgggcggcg ggtgggctgg gtcatggcca ccagtgtgac
     9781 attggccacc agcgggtgaa ttgtggaggc cgcgcggtgg cgattaggtt gccgccatgg
     9841 ctgctgacgg cacattgcgg gccgacccgg aggcgatgcg ggggttcacc gaagcggtgt
     9901 gcgccagcgc agaggacctg cgcaaccggc tggccgctct cgacgatcag gtcggccaac
     9961 tgctgggcgg ctggcgcggc gcgtcgggaa atgcctacgg cgccgcgtgg gagctgtggc
    10021 atcgcggagc cggcgaggtg gagatgggct tatcgatgct ggcacgcttg gtcgccgagg
    10081 ccggcgggat gtacagcgcg aacgaggcgg tgtccgcgca ggaactgcgc gaggtggacc
    10141 gtggctgagc cgtttcgggt ggatacggac gcgttggcgg acgcggtgca gcggatgatg
    10201 gagtttcagc gctgcgccga gcagatgctc accgaaatcg attctctggt aaccaatctg
    10261 cacacgacct ggacgggccg tggcgcggcg gcccacgccg aggcacaccg ccactgggct
    10321 cgcggcgagg cgatgatgcg cgaggcgttg gcccacttgc gaacctcggg cgcgatcgcc
    10381 caccgcaact acacgggcgc gatgtcgacg aatgtgggta tgtggtcgtg acccgcggcg
    10441 ccgggtctcg ccgcttttga tcggcaccgg ctgcgcccgg cggcgtgcac ccataccctg
    10501 gccagacgcg taaatcagct caaacatcgg ccggataggg ttgttaccta ccactgccct
    10561 ggtcaccggg cggcacagcg atctaggagg ttgggccatg gcttcctctg agtcgggcgg
    10621 cgcggacccg atcggtcgcg ttgacctgca gtcgctggtc gacaacgcga gcgcgatacg
    10681 tgcgctccat gacttgcaga ccgcgggcac cgccgcggag ggccgtccgc agccgccgat
    10741 cgatttgacc gcggccgcat tcttcgacgt cgacaacacc ctggtgcagg ggtcctcggc
    10801 cgtacatttt gggcgcgggc tggccgcgcg ccactacttc acctatcgcg atgtgctcgg
    10861 attcgtctac gcccaggcca agttccagct gctcggcaag gagaacagcg aagacgtcgc
    10921 cgccggacgg cgcaaggcgc tcgcgttcat cgagggccgc tccgtcgagc agctgatgag
    10981 gttgggcgag gaggtctacg aggagatcat cgctgacaag atctggcccg gaacccgtga
    11041 gctcacccag atgcatctcg acgccggcca gcaggtctgg cttatcaccg ccacgccgta
    11101 cgagctcgcg gcgaccatcg cgcggcagct cggtctgacc ggcgcgctgg gaacggtcgc
    11161 cgaatcggtc gacggggtct tcaccggcag gctggtcggc gagatcctgc acggcacggg
    11221 aaaggcgcac gcggtgcgct cgctggcgat ccgtgagggc ctcaacctca gacgctgcac
    11281 cgcctattcg gacagctaca acgacgtacc catgctgtcg ctggtgggca cggcggtcgc
    11341 gatcaatccc gacgcgtgac tgcgtgccct ggcccgcgaa cgggggtggg agatccgcga
    11401 cttccggacg gcccgcaagg ccgctcgcat cggggttccg tcggccctgg cgctgggtgc
    11461 cgcgggcggt gcgctggcgg cgctggcgtc gaagcgccaa acacgctgat aagctgcgcc
    11521 gctgagactt attcgagcgg agagtaaagc gccatgacag tccacccaga cgccgaagga
    11581 ctcatcggca gccattaccg ggcacctgat tacttcgagg tcgggcgcga gaagatccgg
    11641 gagttcgcgc tctcggtgaa ggacgaacac cctgcgcact ttagcgagat tgacgccgct
    11701 gcggccgggt atccgggggt ggtggcgccg ctgaccttcc tggccgtggc cggacggcgt
    11761 gtccagctcg agatcttcac caagttcaac atcccgatca acatcgcccg ggtcttccac
    11821 cgggaccaga agttccggtt tcaccggccg atcgtggcca acgacaagct ttttttcgac
    11881 acctatttag actcggtgtt ggagtcccac ggcaccgtca tcgcggagat tcgcagcgaa
    11941 gtcacagatg ctgacgggaa tccggtggtc accagcacgg tcacgatgct gggtgaagca
    12001 gcacaacaag acgcaggcgc tgaagaaacc gtcgccgcaa tagcagcaat atcagcggga
    12061 aagtaggaac gaatcaatga cgtcacaggg tgaaaaccac gcaacgcagc tgaagccacc
    12121 tgttgaagca gtccgatccc actacgacaa gtcgaacgag ttcttcaagc tctggcttga
    12181 cccgtcgatg acctacagct gcggctactt cgacgagaat ccggacccgc aacatctgac
    12241 caagacgctc gaagaggcgc aatacgcgaa gcgcaagctc gctctggaca agctgggcct
    12301 caagcccggc atgacgctgc tcgacatcgg ctccgggtgg ggctcgacga tgcggcacgc
    12361 ggtggccgag tacgacgtcg acgtgatcgg cctgacgctc agcgagaacc agtacgccca
    12421 ttgtgtggcc gagttcgaca agatggacag cccccgccgc aaagaggtgc gcatccaggg
    12481 ctgggaagaa ttcgacgagc cgatcgaccg gatcgtgtcg ctgggtgcgt ttgagcactt
    12541 tgccgacggt gcgggcgacg ccgggtacga gcgctacgcc accttcttca agaagtacta
    12601 cgacttactg cccgacgacg gccgcatgct gctgcactcg atcgtggtgc ccagccgcga
    12661 agagggcaac gcgatgggct tgaaggtgaa catgaccctg ctgcggttca tcagcttcat
    12721 cttgaaggag atctacccgg gcggaaagtt gccccaggtc gatctggtcg acaaatactc
    12781 gacgggtgcg ggtttcaata tcgagcgaca ccacttcatc ggcaagaact acgtcccgac
    12841 gctgaccgcc tggggcgatg ccctcgaggc gcacaaggaa gaggcgatcg ccctgaaggg
    12901 gcaggagacc tacgacatct acttgaagta cttgcgcggc tgctccgacc tgttccgcga
    12961 cggctacacg aacgtctgcc agttcacgat ggtcaagtag tcctcacgta ggacgcggtg
    13021 gcctttccgc cgccccaggc gtaacgctgc tgcgcatttc ggccagattt ttggcagtgg
    13081 cgttacgctc gcgacttggc cgcctcaagg ccgccccaaa gacgcgccaa agcacgccga
    13141 aagctaaccc aagaagatgt tgcggcgccc ggccagcagc cggtagagcg tctgctgaat
    13201 cgtctcgcgc acctggtcgg tcaactcgaa ggtgaccatc ggatcctcgg cgtcggacgt
    13261 ggcgtgatcg ttcgtgtaga tcggctcacc gaacgcgatc cgccacttcg acggcagcgg
    13321 caccaagccc gcaggcccgg ccaacgggaa tagcggggtg accgggaagt acggcagccc
    13381 gaacagccgc gccagcagct tgacgtcggt cagcatcgga tagatctctt cggaaccgat
    13441 gatcgagcac ggaacgatgg gggctttggt gcgcagcgcc gccgacacga agccgccgcg
    13501 gccgaacctc tgcaaccgat agcggtcctc gaagcgtttg cccaggccct tgtagccctc
    13561 cgggaacacg gcggtgagct cgcccgcggc gagcagccgg tgcgcgtccg tcgtgcaggc
    13621 catggtgtgg ccggccttgc gggccgcttc gccgatcacc ggcaggtcga acaccatgtc
    13681 ggcggccagc agccgcaggt cccggtgcgc cggatgttcg tcgtgcaccg cgaccgacag
    13741 catcagccca tcgaagggca gcacgccggc gtgattggcc accagcagcg cggccccgtc
    13801 gctcggcagg ttctcgatgc cgctgacctc gacccggaac cacgacctga agaagaacct
    13861 cagcaaaggc cggacgatcg cgctgttgaa gtgcggatcg aacccgaact cgtcgacggt
    13921 gtagtcaccc gcgagccgct gccggaggaa accggcgacg gcggcaacgc gctgcgcgag
    13981 ctcgttcagc ggggcctcgg ccggcggcgt cgcgcccgca gcacgccggt gctcatcgat
    14041 ttcgcggacg acggcggcga tctgttcggc ggacgcacga ccattgggat cggacaggag
    14101 cgaagggtgc tgacgggacg attccgcccg ctgatcggct ctgcgacgcg ccgctacccg
    14161 agccctattc gtgtgcagtg gaatgacgtt cgccctggtt tcacccgcca cgatatctac
    14221 ctgaccccac cccgtggaat tggatttcgg ctaccccagc gctgcgccaa cgctatggcc
    14281 cgaccctcca gggagcgtac ccgatgcggg tcgattatgg gagtgaggcc gcggccgcga
    14341 acgtagtcgt cgaacgcctc ggcggtggtc catttcggct ggtagccgag ctcagagcgc
    14401 attctcgtgg tgtccatgac tctgccataa ctcaaataat tgaactgatc gcgggtgacg
    14461 tccgcatagc gattggcccg cctcagcgaa tccagcgccc agacgccaaa cccgggtacc
    14521 ggcaaaggga tacggccggc ccgccggatc gcctgcgaca gcatgatgat gccgtcggcg
    14581 ccgatgttga acgtgccggc cttgccggcc atcgccgcgc gctcgagcgc tcccagcgcg
    14641 tcctgctcgt gcagcaactg cagccgcgcg tcgcggccga acagcgtcgg gaccaccggc
    14701 ccggccaggt agcgcgacag cgtggtatcc atcgccggac cgatcatgtt ggccagccgc
    14761 agaatcgtca ccgcgatatc gggtctgcgc cggcccagtc cccgcacgta accttcgata
    14821 tcgaggctgt ccttggcaaa gccgtcccgg aagggtcgac ggctgctgct gtcctcggtg
    14881 aacatgaccg gatcgtgcgc ggtcgacccg tacacctcgg aggtcgactt cagcacgacg
    14941 cggcgcaccg tgggcgcctt ctggcaggcc gcgaaaagtt gcatcgcgcc catcacgttg
    15001 agttccttca acgccgcgct gccgccggat cgcggcgcgt aggatgccgc agcggcgtgc
    15061 accacggtgt cgacgtcacc gttgcgaatc accttcgcga tgaatggatt gcgaatgtcg
    15121 gcgcggacga actcggcccg gcccatccgc cgcagcatgt ccttgctggg ggcgatcgcg
    15181 tcgaccgcga tgaccccttt gatcaagggg tcctgcacca gccgagcggt caggtaaccg
    15241 cccagaaacc ggcaggcacc ggtgaccagc acaattttgg ggtaatgcac ggtgttggtc
    15301 gcggtgccgc ccgtgccgcc accatccccg ttcgactgat ccactcaaat agcctaacgg
    15361 gccggcaaga cggcggcgtt acggcggggt gagggttggc gcgggttact tgccaagttt
    15421 tctgcgctgc acccgggtgc gacgcaatag cttgcgatgc tttttcttcg acatgcgctt
    15481 gcgccgcttt ttgattactg aacccatgaa ctccgctatc caacccgtga ccgcttgaga
    15541 cgactataaa aggaccccgc cactttaccc ggccggcggc gtcgaacacc aaaccggcag
    15601 cctcgtgacc gttggcaagg ccacgtggtg cccgatgcgg tgacggtcac gcccggaaaa
    15661 tctggacgat ctcgcggcgc ggcaggagtc aggtcagccc gcgtcgaagt acgaggtctc
    15721 cagcatgtcg tgcacggcct tggcgtgcac ccggaaggag cgccccaccc gaacagccgg
    15781 cagctcgccg ttgtgcacca accggtacac cgtcatcttg gagacccgca tcagcgcggc
    15841 cacctccgcg acggtgagaa attgtgtcct ggcctgctgg ccatcgccgg aactggtgtc
    15901 ccggggctta ccagcggaat ctcgcgccga tggcccgttc gtagacgtca tcgcaaccca
    15961 atcgtgtcag gcccgcgcaa tgccagcggc ttcccctccg ctggcaccca cacgtgctta
    16021 caaagaggag aatagcggga ctagtggggt tactggtact ggtgggggat aatcaattcg
    16081 aaattttaaa attattcaga tgtaattctt agctgttcag agcgcctttt ggcggcctga
    16141 accgccgcgt cgacagccgc ccgcaaaccg cctcgttcca gttcgcgcag cccggcagcg
    16201 gtggtgccac cgggcgaggt gaccgtcgcg cgcagctgcg ccgccgtagt gtccgcccac
    16261 cttcccgcct cctcgccctc gacgcctcgc cggtcggaat ccatccgcgc cagcagcatt
    16321 gccgccgacc cggccatcgt ctgcgtggtc aggtcggtgg cgacctcgcg actcagaccc
    16381 gccgcgacac cggcgtccac cagggcctcg accagcagaa agaaatacgc cgggcccgag
    16441 ccggacagcg cggtcacggc atccatctgg gattcgggaa ccgtcagcac gccgccgacc
    16501 gagtcgaaca gggccgacac cccctcgagc tgtggcgggg taacgaaacg gcccttggcc
    16561 agggcggtta cccccgcacc caccaacgcg gccgcattcg gcatcgcccg aaccaccgac
    16621 gttccggccg gcagccgggc ttcgaagtag gagatcgcga tccccgccgc gacggtgacg
    16681 aagacctgct cggcgctgtc gttctcggca gcggcggccg cgcgggcgag ctcccccatc
    16741 accgagtcga cgtcggccgg ctttacggcg acgacgacga atgtcgcgtt gtccaccgcc
    16801 tcggtcaccg acgtcgacaa caccgaatag gtatccgcga ggtatttggc gcgctccggc
    16861 acccgttcgg ccaccaccag gtctttcacc tgctgcccgg agcgcagcag gcccgacagc
    16921 aacgcctccc cgatgctgcc gccaccgatg atcgcgattc ttgccatgcc ggaaagcatc
    16981 gcagacgggc cagccgatcc gcgctaccgg gcaggcacca tcgccagctg gcgcgactgg
    17041 accacgatgc gtcccgagct gtcgaccacg gtgtggtcct cgtcgaacca gtcgtgcccg
    17101 atctccacgc aggtgcacac cacccgcagc caaccatcgg ccgggaggcc gcgaaggaag
    17161 gcggtcagct gaatggtggg cgcccagccg gtgcggtcca ccgcgaaggt caccggcgcc
    17221 gacagatccc cgcacatcag cgcaaacagc gggtccgggg ccacgccgcg ggggcgtgcc
    17281 cacatctgga tcaccggcgg ccggccctcg gcactgggcg ccagggtgga cagcagcggc
    17341 cgcacgtcgc agccttcgcc caggtgcacg agcccggcca gcgggtggcc gggcccgatc
    17401 ggcgcgacat cgccgggcgg ttccggtggc atcaaatcca ggaccgggtt cgccgacagc
    17461 agcggcacgg tcccgtgctc gggctcgccg aggttgacga cggcgtgcac cgcggtccgg
    17521 tcgccctgaa tcagcgcgac atcaaccaca ctgatccggc ggccgcgctt gcggatcaac
    17581 gtcgtcagca acatcggccc cgggtcgggc gcccacagga agcttgccga cacggacacc
    17641 ggctccagga ccggccccag cccgtccgat gatccgccgg cgcaggcggt gcgggcggcg
    17701 ttggcgcaca gcgccagcat cgcgccgccg tgcactttgg gcccgatggt ccagtgcttg
    17761 tcgagctccc cctcgtatgt ccccggctcg acctcacaca gcgtcattgc gctggtgaac
    17821 aacgcgttcc tggtcaccgc atcagatggg tgcgggcgaa ctgcaacgac tcgacgagca
    17881 tcgcctcgcg ctcgtaggcg gagcgcgcac tcgacgtgga cacctccaga atcacatggc
    17941 cggcaaaacg gcctgcggca agcagctgac acacctcggc ggtgggttgt gtgccgcgcc
    18001 ccggcaccaa gtgctcgtcg gcgggcagac cgctgccgtc acacaaatgc agatgaacca
    18061 gaccctcgcc catccgctcg gccatttcca gcgagtcggt gcccgcggtc gcggtatggg
    18121 acagatccag cgtgtagtgc gcgtgattgc cgtccagcgg gtcgtaggac ggcgcgaacg
    18181 ccgagatcgc cggacccggg ccgccgccac gccggcgcat ccgttcccgc gactggccgg
    18241 ccccgaaaaa ccggtccgcc cggaagggga acatgttctc cacggcgatc atcacgtcgc
    18301 tggatgcttc cagctccaag acctgctcgc tgaatccttc ggcatagcgc cgttgccagc
    18361 ggaagggcgg atgcacgacg acggtctgcg ctcccagctc ctcggcggcc cgcacgctgc
    18421 gttccagctt gagaatcgga ttggagcccc acacccgctg cgagatcagc aggcagggcg
    18481 cgtgcacgga cagcaccggc acgcgatagc gctcggacag gttcttgacg gcctcgacgt
    18541 cctggctgac ggactcgctc cacaccatca gctcgacacc gtcgtagccg agcctggccg
    18601 catactcgaa cgcggcctcg gcccgcaatg ggtacaccga ggccgtcgac aggccgactt
    18661 tgattgctgg gcgcaaagtt ctgtctggtt ccgagtaggt ccgtagatct gccctagccc
    18721 gactgcaacg acaacgccaa cggccccagg gtgatcagcg cccccaccgc gaccgcgatc
    18781 aacgtactcg cgatgtcctc cgtcttgcga accacccgca cccctaccac caaaccgagg
    18841 atgaccagca ccgagagcac caatgccacg atgctgttcc aacgccacag ctggtcgaag
    18901 gccacgaaca agccggcccc gaacgcgaca gccagaatcg actggaacac gatcacgctg
    18961 ccgcgccaca acgcgtcaag ccgctcccgc acgccgtgga cccgggcgtc gcccgcgccc
    19021 ttggccttgg tgtcgggacg atcctcgtcg actttgcgtt cggcgtccag ttcagccgct
    19081 tcctgctggc cgccgcgccg ccgggcgacc tcatcggcca gggtctgccc gccgaacagc
    19141 atggaatccg actgcaggta ggagcgcacg aaggcggaat cctcggccgc caggtcggcg
    19201 tcgcgaacct cggagtccat cacatcgacc ggaacgtcgg cgtagttgtc gacggggtcc
    19261 ggactcatgt cctcggcacc ggattggctg gtccgatcgc ccgactgctc cgcatagggc
    19321 aggggccgcg ggtatgcgct ccgctcggga cccgagcgtg ggggctgcgg cggtgacttg
    19381 ggccaacgcg gctcgggttg cgaccagtag gcctttttgg gcggttcttc ggttaccggc
    19441 tcgggggtgg gttccgtggg cgccgattcg acaacccggg cggcgccgtt ggcgccgcgc
    19501 tccgccttgc cgtgccccgc ctcgtcgtgg tccgtctcgt cgtgatggtc acggatgacc
    19561 ggaatctcgc cggtcagctc ggcaaccgtg accgcgtcgc tgtccccgcg ccgacgtcgc
    19621 cgacgtcggg tgaccgccgg ggagccgacg gttccgttct tggccagcaa ctcggccact
    19681 gagatcggcc cgctgccggg ttcctccttc tcggagtgtg gtccggtcat cgtttgtcgc
    19741 ctctcgatcg gttcgagttc cgatcaatta cctgacctcc agcatcgcgc accgggctct
    19801 cgacgagtgc agatccgtcg gcttcgctgt ccagtttgcg caagatgaga ccttcccgta
    19861 gcgcccacgg gcagatgtcc acctcttcga tcgacagcgt tcgcatgctt gcctccgcca
    19921 ccagggcgcc ggccacgatc tgcggcgccc gctcggcgct gactccttcc agttctgccc
    19981 ggtcagcggt cgtcatccta gagatgaaag ctatgagttg cctgaggccg tttgccgtaa
    20041 gtgtcctctt cacccggggc ccggcggccg acggcgccgc gccggtgagg cgcgccaacg
    20101 agcggaacgt tttcgacgtt gcgaccgcca gatcagggcc acccgcttcg agcacggtca
    20161 cgccggcttc ggctaattcg gcgtcgagcc aatcccgcag catcgccacc cggcgccggc
    20221 cgggcgggtc gtcggccagc cattcgcggg tcaatcgtcc ggcacccagc ggcagcgaca
    20281 acgcgacctc ggggtcctcg tcgacgccac tcgacaattc cagcgaacca ccgccgatgt
    20341 ccaggttgat gattcgcccg gcgctccacc cgtaccaacg gcgcaccgcc aggaaggtca
    20401 gccgcgactc atcgacgccg cgcagcacct ccagctccac gccggtctct ttgcgcaccc
    20461 gggccagcac gtcgtcggag ttctcggcgt cccggaccgc ggacgtagca aaggccatca
    20521 gttccgcgca gccggagctc accgcgatct tggcgaactc gtcgatggtg gagatcaact
    20581 tgtcggcgcc gcgcttggtg atcttgcccg agctgtcggt ggcctcggcc aatcgcagcg
    20641 tggccttggt cgagctcatc ggcgtcgggt gcccgccacg gtgcgcgtcg accaccaaca
    20701 gatggaccgt gttgctgccg acgtcgagca cacccaatcg cacaggaaac aaacctagtc
    20761 gtgcgacgat gcacgccgcg aagcggagtg agaaggaggg cggcaatcca gcaccagtcg
    20821 tgcgacgatg cacgccgcga agcggagtga ggtgggggta cccccagccg cgaagcggcg
    20881 aggggggcga gggcggcaat ccagcaccag tcgggcgatc gatgcaacgg gtgcccccgg
    20941 atcggggccc gtcagcaccc gctgtccacg accccacggg acgagtgtct gcgagtcaca
    21001 cagccagatt cgtggtctaa cgttggcaac gtggcgaatt cgcatccgca gccgggtcaa
    21061 gaggtcgaat tggacttcgc ccgtgagtgg gtggagttct atgacccgga caatcccgag
    21121 cacctgatcg cggccgatct cacctggctg ctgtcccgct ggacatgtgt attcggcacc
    21181 ccggcctgcc gcggcacggt ggcggggcgc cctgacgacg gatgctgctc gcacggcgcg
    21241 ttcctgtccg acgacgacga ccgcgcccgg ctggacgatg cggtcaagac gctgaccgac
    21301 gccgattggc aattccgcga caagggcttg ggccgcaagg gttatctgga actcgacgag
    21361 cacgacggcc aaccgcaata ccgcacccga aaataccggg acgcctgcat tttcttgaac
    21421 cggcccggct ttgccgccgg cgccggctgc gcactgcata ccaaagccct caagctgggc
    21481 gtcgcgccgc tgaccatgaa acccgacgtc tgctggcagt tgccgatccg gcgcagccag
    21541 gagtgggtga cccggcccga cggcgtcgaa atcctcaaaa ccaccgtcac cgaatacgac
    21601 cggcgcggct ggggctcggg tggtgcggat ctgcactggt attgcaccgg cgatccggcc
    21661 gcccatgtcg gcgccaagca ggtgttcgaa agcatggccg acgaactcac cgagctgctc
    21721 ggcgccaagg cttacgcgga gctggcggca atgtgtaagc ggcgcagtaa gttagggctt
    21781 atcgcggtcc atcccgctac tcggctcgcc gagtagcggg gtggtcgcaa cgtttgggag
    21841 ggggggtcgt ggcagctatc aaccagccgc gggcggagcc aggatcagtc cccacccgat
    21901 ggctgggtgg gcgcggcgtc tggctcaagc gcgggccgag ggaagacgcc gcggatctcg
    21961 tattgtctga cggaccgcac gaggggtgag ccgatgtatc tcgcagtggc ccggctcggt
    22021 ggattcggcg accagacggc ataggtcacc cgtcaaacgg cgctgaacgc ctgcgggatt
    22081 tatcgtcgcg tgaggcccag ccgtccgcca ccggggcatc ctgattgggt aggacgatgc
    22141 cgtcgtttgg ctgcccgccg tcgactccga ccgattccgg tcgtaccggc gtacgccctg
    22201 atttctggcc gggatgccgt cccgggcgct ggggcaagag aatgaccaga gcgacgatta
    22261 tgacggcaat gaccgcggaa gccagcgggc ggctcagatt caggccgcca tggctcaccg
    22321 gcttatcgag gaaatcgccc acggtggcgc caagaggccg ggtcaagatg aacgcgaccc
    22381 agaacagcgt gacacgcgac acgcttgtcc agtaatacag ggccgcgacg gcgacgaggc
    22441 ctgcgccgaa taccagcgca ccgccttcgt agcccagccc gccgtcatcg gcgagccaat
    22501 ctccgagtgc ggtgcccaga gtctgcgaaa acgtgatggt ggcccagtag aacgcctcta
    22561 ccttcggcgt cgaaacggta ttgaccgaca ccgttccctc cgaccagtac cagagtccca
    22621 gggtcgccaa cagacaacac aacagcagaa gcgatccacc cgtgtagccg atccccagcg
    22681 atcggtcggc gaagtcggct aacgtcgtgc cgaacgtcgt cgaggcgacg atcgtcgccc
    22741 agtagagggc cggatggaaa cgcttggcga agatctgcgc gacaacgagc gccaccagcg
    22801 cgccgccgaa cagggcgacg ccggccagat aaccccaatt cttgtgcgta tcagcgtgca
    22861 agatggtcat cgtcacggcg tcgccgcccg tctcgcccag cgtcgtcgcc aaaaccttga
    22921 tcacccaaaa gcccagggtg acagccggta ccttggtcaa agcctgtttc gtcgcatcat
    22981 tcatggaatg acggcctccc gttttcccgg agcggctagg cgtgcgctgg tgaacggtcg
    23041 tcctgatcgg gcaacccgcg tgacgcatcc cacccgcata cccgccgccc cgttgaggcg
    23101 ggcgctgtca gacctaagct aacgagctcg gactgagact tcgctgagga acactgtgtt
    23161 ggctcaatac gtgctggtag cggtgtttac ggtaccactg cgacgcgggc ggcctggttc
    23221 ttcgcaggct ccctccgccg gtgtttcaca ctgggctggc ggtcatgagg cgcgcgcgtg
    23281 gtggatgcga cccgatccca accggcgaga aaatgatgat gacgggcgaa cacccgctgg
    23341 gcgaggagag ctatgagttg cacacgtggg cgatgtaacg gacggcggtg ttaggccggc
    23401 tgccgccgtg gctgcgcccg cgatactggg gcatcgcggc gcgttcggcg tttgtgtcgg
    23461 ccacggtggt gttggtggcg ctggtcgtag caggtggcgg tcttgtggtc gtgctggatc
    23521 gctcgctgac agccagcctg gacgccgccg ccgccgcgcg cctgcaagac gtcgcagcgg
    23581 gcctgcagac agacaccgcg ggcgaacttg acgcccggtt gctggctacc gatcagcgca
    23641 tcgttggggt gcaggtgatc gacgcggcag gggcggtgat cgcgcgttcc ccgtctacgc
    23701 cggtcgcggc gctggctccg atcgaagtgg cggggtcaaa cccgcgcgtg ggcatccctc
    23761 ccgccaacgc gctcaccgac cgcgacatgc gcatcgccgc acaaaccgtg agcagcgctg
    23821 gcgggcacta cacggtgctg gtggccgtcg gcaccgaagc ggtcgaatca accgttagaa
    23881 ccatcgggtt cgggctcgcg ttagcggcac cgatcgtcat cgcggtggcc gcggcggcaa
    23941 cctacttgtt ggtgggacgg tcaatgcgat cggtggaggc gattcgggca cgcgtcgccg
    24001 aaatcagcgt ctccgatttg gccgggcggg ttccggtgcc cgagcaccgc gatgaaatct
    24061 cggcgctggc ggtgaccatg aacgcgatgc tgtcgcgcat cgaatccggt caggccgcgc
    24121 agcgccggtt cgtcgcggat gcttcgcatg aaatgcgcag tcccctggcc accgtcatct
    24181 cggccctcga ggtgggcgtg gcccatccgc aactgctcaa cccgcagctg gccagcacga
    24241 cactgttgcc cgaggcgcac cgcatgcaat cactggtgga ggatttgctg ctgctagcgc
    24301 gggccgacga acgcgggctt gcgttgcgcc acgacgacgt cgacctggat gacctggtcg
    24361 ccgccgagac cgcacggctg cagcgcgaaa ccgcgctggc cgtccatatc gatgtgaccc
    24421 cggtgcgggt gcagggggac gcgagcgcaa tgtcgcgggc cgtgcgaaat ctgttggata
    24481 atgcggcccg tcacgcctat tcgcgcatcc acgttacggc tcacactagc gacgggcacg
    24541 ccgagttcac ggtgtcagac gatggccctg gcatatccgc gccagatcgg ttgcgcgtat
    24601 ttgaccggtt tgtgcggctg gataccggcc gcgcccgcag cggcggcgga accgggttgg
    24661 ggctggccat cgttgctgag atcgttgccg cacacggcgg cagcgtccgc atcgccgaac
    24721 ccgccggcgg cggaacggtg gtaattgttc agttgccggt gggaaaatcg cccgattcga
    24781 gacggtagcc gacaccgcgg atggtatcga ttgtgttggt gccgaacggg gcgtcgatct
    24841 tgcggcgcag atagccgacg tagacctcca cgacgttgtc tgctcccccg taatggtgat
    24901 cccacacgtt gtgaaggatg ttggtctttg tcacgacggc gtccttgttt cgcatcaggt
    24961 attccagcag cgcatactct ctgggcgtca gtgagatcgg cgtggaagag cggtgaactg
    25021 cgcgccgcac cgggtccagc gacaggctac cggcggtcag taccacgggg cgttgcgggg
    25081 cgccacggcg catgagggcc cgcagccggg caacaagcac gatgaacgaa aacggcttgg
    25141 tcaggtagtc atcggcgcct aaatccaacg catcggtctg atcgtattcc ccgtccttgg
    25201 cggtgagcat taacaccggc gtccagatct ctttggcccg cagctgccgc acgacctcgt
    25261 acccgttcat cctcggcagc atgatgtcca acacgacgac gtcaaacgaa tgctcgcttg
    25321 cctgccacaa gccatcgatg cccgtggtcg ccgtgacgac cacaaatccc tcggcgcgca
    25381 accccatcgc cagggtcgcg accagatgtg gctcatcctc gacgacgagg accttcaccc
    25441 gagtttccct cctcgactgg cggccagcgt tgctcggccg ccggtcccag tgtggcgcac
    25501 ccagtatcgg ttagccgaga ctgcggtccc cgacggcact ccaatgagaa ccgtcaaaac
    25561 cacagtctcg gcctacgcac cgcctactag tcgtccggcc cgtccgggga atcgccgggc
    25621 tcgggcaagt ccggcgcgtc gggcccactt gccggcgcga ccagcggcac gtgcgtcggc
    25681 gcaccgacag ccacggcagc ggcccccatg gcgccgagca cgatcgccga ccccgcaagc
    25741 gcgatagcaa cccgctttct gatgttcatg tgtctgtact cctttcaccg gcgacatcca
    25801 ccgacgtcgc ggccaccaga ctggcatccc ctcgctgagg aaacgctgag acattcagcc
    25861 gctcagccac ttcgggctgc agcgttcgct gacggcggcc atgatgactg cggcgctgat
    25921 atgatcggca tcgaaaactt ctgagcagca atcggtttca ctaccctgat gaagtcgtgc
    25981 tcagcgacgt ggtaatgctc atggcaacaa ccaccatcaa tcagcgcggt tcgtagctca
    26041 cccggtatgt cggattgggc tacgggtgcg gcgagctcgg tgtggtcggg cctatggaca
    26101 tcgaaggctg cgggcccgct tcgctttgcc cccacgactt ttcgccgtgg tgaccatcga
    26161 cctccggtga gccttcgccg tatttatcat cgatgcccgc cgacgattca ccatggcgct
    26221 cgtaggcacc agattcgccc cggccgaagt ggctaccggc gcagaccccg gacaaaaaga
    26281 tcaccccgac gacaaatacg gccccggcta cgatgcccgc ccaggccgcg agctggctga
    26341 gtctgttggg ccggtcacgc cgctctgggc ccatgaccgc gggctctatg cgagattctg
    26401 gtgtttcagt cactatcgtt tgtcccttcg tcttagtgcg cagtagttat cgacaccact
    26461 gtgggagcgc cccgctgaga gttcgctgag catcgctgag ggctcttccg gtaacgaccg
    26521 gctcgacggc caaccggggg ccgcggctgg cgcaagaact cggcggtccg ccgcgactct
    26581 tggggtcaga ccgcttctca gcgtgggctc agccacgtgt ctgtacggtc gggcgccatg
    26641 accgagacaa cgaaacccga ggccgtcagg gcccagcgcg cactgctgag caaggtgccc
    26701 gaggtcacgg tttggttctg gctcatcaag atcctgtgca ccaccgtggg tgagagcttc
    26761 gccgactgga tcaacacgac gctgggtttt ggtctgacca tgaccgcgct cgcctttgcg
    26821 gcgctacttg ccgcggttct cgggtggcaa ttgcgcctca accgctacgt cgtgctcgtc
    26881 tactggttaa cggtggtcgt gctcagtgtg accggcacgc tctacaccga catcctcacc
    26941 gacaagctgg gcgttccgct cgcgctgagc acaagtgtct tcgcggcggc cctggcagtc
    27001 atctacgcgg tgtggtacgc gcgcgaacgg actctgtcga tccacagcat cgtcaccctg
    27061 ccccgagagt tgttctactg ggtggcggtg ctggtcacct tcgccctggg cacagccgca
    27121 ggtgactgga cactgcagct caccggctgg ggaccgggca ctgctgtgct gctgccgact
    27181 ggcctgatca tctcgatcgt gatgggctgg cggctgggcg ccaacccggt actgtccttc
    27241 tggctcgcat acaccctgac ccgtcccttg ggtgcgaacc tcggcgactg gttcgcttcc
    27301 ccgtcgagca gccacggcct cgggttgggt accgctgcca ccagcgcgct cttcctcacc
    27361 gcgatcctcg ctacggtcgt gtacctaacc cgcacccgca gcgacgtcat cgaagactac
    27421 gaccgaaccc acactccgat cgtcaccaca aatcccggtc gggagcggat catgctcagc
    27481 tactacgccg ccgtcgctat ggccaccggc gcgctgctgg tgtgggccgc cggacaacac
    27541 cacgccaccg cgacaaccaa cgacgatcac gaagccgctg ccgcgcccgt gaccgcgcaa
    27601 cctctttcgg ggcaggcgac cgcacatttc ccgccggcag agatagcaaa attccgcact
    27661 attagccagg acaccctcga caagctgcgg gcaggcgacc aggccggcgc gactgcccgg
    27721 atcaacgacc ttgaaacggc ttgggacggc gcccagtcga ccctacagcc cttggacgaa
    27781 aacgcctggc aggtcctcga ccgtcagatc gacaacgcgc tcaaggcgtt gcgggccagc
    27841 agacccgacc ccgcgaccga gaggcagact ctcaccacgc tcttgacggc ccttcaatga
    27901 ggcaaccgcg gaacgtgcgc ggagcctagt gactcgtcga
//
LOCUS       DASSGQ010000011        21221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_426021,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000011 DASSGQ010000000
VERSION     DASSGQ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 21221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21221
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426021"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..97
                     /locus_tag="VFI55_00410"
     CDS             <1..97
                     /locus_tag="VFI55_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3046 domain-containing protein"
                     /protein_id="HET7072530.1"
                     /translation="RTAARAIEDGVEPRDVWRALCVDFDVPRDQW"
     gene            complement(231..908)
                     /locus_tag="VFI55_00415"
     CDS             complement(231..908)
                     /locus_tag="VFI55_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7072531.1"
                     /translation="MDISIQLAEISSYRGFFTLTVRGDARGWHPVRRSYDHGFADLIA
                     ATARRYHTTDLRIGASLVHLGHATRLWSPVLACTLGHGVVPDLTELQRADDGAQLRMP
                     EPVGEAVNAPSADFLYRIVVQQHLEPLAAGLPVKLAHALLAGNIASALVGASRALLSA
                     RPDLRRAIVAITNSLLDTGTLAGSGLITTPHLGFRRRSCCLFYRVPGRSVCGDCVFRR
                     TPRPNRS"
     regulatory      complement(950..1095)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1281..2321
                     /gene="recA"
                     /locus_tag="VFI55_00420"
     CDS             1281..2321
                     /gene="recA"
                     /locus_tag="VFI55_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET7072532.1"
                     /translation="MAQAPDREKALELAMAQIEKNYGKGSVMRLGDEMRQPISVIPTG
                     SIALDVALGIGGLPRGRVVEIYGPESSGKTTVALHAVANAQAAGGVAAFIDAEHALDP
                     DYAKKLGVDTDSLLVSQPDTGEQALEIADMLIRSGALDIVVIDSVAALVPRAELEGEM
                     GDSHVGLQARLMSQALRKMTGALNNSGTTAIFINQLREKIGVMFGSPETTTGGKALKF
                     YASVRLDVRRIETLKDGTNAVGNRTRVKVVKNKVSPPFKQAEFDILYGKGISKEGSLI
                     DMGVDQGFIRKSGSWFTYEGEQLGQGKENARNFLMENGDVANEIEKKIKEKLGIGAVV
                     TDDDILPAPVDF"
     gene            2287..2808
                     /gene="recX"
                     /locus_tag="VFI55_00425"
     CDS             2287..2808
                     /gene="recX"
                     /locus_tag="VFI55_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HET7072533.1"
                     /translation="MTTSCPPPSISEPSREEQARALCLRLLTARARTRAELSGQLAKR
                     GYPDDVTDRVLARLAAVGLVDDVEFAEQWVESRRSSAGKGRRALAAELHTKGVDNEVI
                     TTVLGGIDTGAERQRAEQLVRARLKREDLDGDSTRVARRLVGMLARRGYDQNMAYDVV
                     RVQLNVELERRRT"
     gene            2876..4393
                     /gene="miaB"
                     /locus_tag="VFI55_00430"
     CDS             2876..4393
                     /gene="miaB"
                     /locus_tag="VFI55_00430"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HET7072534.1"
                     /translation="MVTQDAAARAAAPARTYQVRTYGCQMNVHDSERLAGLLEAAGYR
                     RAADGADADVVVFNTCAVRENADNKLYGNLSHLAPRKRGNPDMQIAVGGCLAQKDRDA
                     LLRKAPWVDVIFGTHNIGSLPTLLDRARHNKVAQVEIAEALQQFPSSLPSARESAYAA
                     WVSVSVGCNNSCTFCIVPSLRGKEVDRSPADILAEVQSLVDDGVLEVTLLGQNVNAYG
                     VSFADPALPRDRGAFAELLRACGRVDGLERVRFTSPHPAEFTDDVIEAMAQTPNVCPA
                     LHMPLQSGSDRVLRAMRRSYRAERYLGIIDRVRAAMPHAAITTDLIVGFPGETEEDFA
                     ATLDVVRQARFAAAFTFQYSKRPGTPAATFDGQLPKAVVQERYERLVELQERISLEGN
                     RALIGQTVELLVAAGEGRKDSRTARMSGRARDGRLVHFTADGRVRPGDIVTTVVTEAA
                     PHHLIADAGIRTHRGTRAGDAHAAGQRPGSIGLGMPTVGPSAGPSESTPSLGCAR"
     gene            4381..5016
                     /locus_tag="VFI55_00435"
     CDS             4381..5016
                     /locus_tag="VFI55_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072535.1"
                     /translation="MCPMKKDETDLDALRTAIDAAERRVAGEIEPGSRALVVAILVFV
                     LLGSFILPHTGDVRGWDVLFGSHGASAAAVSLPSRVFVWLVLVFGVGFSMLALVTRRW
                     AVAWLALAGTSIASAIGLLAVWSRQTSPGHPGPGVGLIVAWITVIVLTFHWARVVWSR
                     TAVQLAAEEQRRQVAAQQQSKTLLDTFGTDTTSPGEPDNPGDPDAGRPAGP"
     gene            complement(5020..6351)
                     /locus_tag="VFI55_00440"
     CDS             complement(5020..6351)
                     /locus_tag="VFI55_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HET7072536.1"
                     /translation="MTADKPISNDSPRPAPRPGPRPGPRPLSSGPRPTTHAVATHPPS
                     NPHQFGRVDDDGTVWLISSAGERVVGSWQAGDPEAAFAHFGRRFDDLSTEVTLMEERL
                     TSGTGDARKIRANASALAETLPTASVLGDVDALEGRLTNLIARVDVIVSADRSRREGQ
                     RAALVSRKEALAAEAEDLAANSTQWKVAGDRLRAILDEWKTITGLDRKVDEALWKRYA
                     AARETFNRRRGSHFAELDRERSGIRQAKEELCERAEELSASTDWTATSTEFRKLLTEW
                     KAAGRASKDVDDALWRRFKAAQDAFFAARNAVTAEKDAELHANATAKEALLTEAESID
                     TSNRDAARAALRSIGEKWDAIGRVPRERSADLERRLRAVEKKVREADETDWSDPQAQA
                     RAEQFRTRAEQYEQQARKAAAAGRTKDAEQAKANAEQWRQWADAAAEALRR"
     gene            6726..7667
                     /locus_tag="VFI55_00445"
     CDS             6726..7667
                     /locus_tag="VFI55_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7072537.1"
                     /translation="MAKVDIAALLALVAALISGVGDVVRQRSAHEITEEKVGHVELLR
                     MSLRDTRWWLGGGAAVLSAALQAAALGLGSVVLVQALQVTALLFALPVSAWVTKKALT
                     RREWGWAVLLATAVAVFVMVGEPAAGYQRASLQTWAIVAVLIAPVMAFCVLGARTCSG
                     PPAAVLLAVVSASSWALFAVFTKAIVDVADGGLWALLRTPELYGWLLVAALGTVFQQS
                     SFRASSLTASLPTMTVTEPVVASVLGVTVLGETLGADGPKLIVLVASVVVVIVATAAL
                     ARGEAVSLASGDEGQLRDRPEPEAAASPVVVGATSHA"
     gene            7793..8716
                     /locus_tag="VFI55_00450"
     CDS             7793..8716
                     /locus_tag="VFI55_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7072538.1"
                     /translation="MSKVDVAALIALCAALASAIGDVIRQRSAQEITDKEVGHLELFR
                     MSLRDRRWWLGGLAAITNYSLQAAALAWGSVVMVTSLQVAALLFALPIYARLSHNRVT
                     RWEWIWAVVLACALAVVIIVGDPEAGQQRASLSTWLVVALVMVPALVLCVLAARIWSG
                     RPIAAVLLAVVAGSSLALFAVLTKGVVEVGEEGLGAVLRAPEFYPWLLVALCGMIFQQ
                     SAFRAGALTASLPTMTVAKPVVAGVLGVTVLGETLGATGPAAFVLVAAVAMVIIATVA
                     LARGEAASMAPRTRRDRKGSSSQETPLEAPL"
     gene            8800..9486
                     /locus_tag="VFI55_00455"
     CDS             8800..9486
                     /locus_tag="VFI55_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072539.1"
                     /translation="MLSAIAIIPSAPVLVPELAGAAAAEVADLTAAVKAAAALLPPRW
                     VAVGTGRADEVVGPSATGTFGGFGVDLPVRLSARDDGLPIEFPVCALLAAWVRGQVRP
                     DADAQVRVYRGDQDAETALARGRELRAEIDRAPDPVGVLVVADGANTLSPAAPGGYDP
                     RNAESQLVLDDALASGDVTALARLPHQIVGRVAFQVLAGLAEPGPRSSKELYRGAPYG
                     VGYFAGIWQL"
     gene            9483..10445
                     /gene="miaA"
                     /locus_tag="VFI55_00460"
     CDS             9483..10445
                     /gene="miaA"
                     /locus_tag="VFI55_00460"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET7072540.1"
                     /translation="MKSTGRPLAIVGPTGTGKSQLALDFAERVAQLGGEVGAEIVNAD
                     AMQLYRGMDIGTAKLPVEQRRGIPHHQLDVLNVTETATVARYQRDAAADIEAIAARGA
                     VPVIVGGSMLYIQSLLDDWSFPATDPAIRARWEQRLAEVGVGRLHAELARRDPGAAAA
                     ILPTDGRRVVRALEVVELTGQPFAASAPRIGAPRWDTVIVGLDCDTAILDERLARRTD
                     TMFDQGLVDEVRALLREGLRDGVTAARALGYAQVLAALDAGAEAGPSAVALRDAREQT
                     YVGTRRYVRRQRSWFRRDHRVHWLDAADRAGLVDGALRAWRHVS"
     gene            10450..11334
                     /gene="dapF"
                     /locus_tag="VFI55_00465"
     CDS             10450..11334
                     /gene="dapF"
                     /locus_tag="VFI55_00465"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HET7072541.1"
                     /translation="MEFAKGHGTQNDFVLLPDLDAELTLTAARVAAVCDRRRGLGADG
                     VLRITTAGVALAVGVLSRLPDGVGVTDWYMDYRNADGSTAQMCGNGVRVFAHYLRASG
                     LEARDEFVVGSLAGPRPVTVHHVDDTNAEVTVDMGKANRLGTGDAVLGGRRFTGLAVD
                     VGNPHLACLDPRLSAEELAALDVGAPVQFDRAQFPDGVNVEVLTSPVWGPAGGAVQMR
                     VHERGVGETRSCGTGTVAAAVAALADAGADTGTLTVRVPGGDVVVTITEATSYLRGPS
                     VLVARGQISEEWWRTQQR"
     gene            11381..12832
                     /gene="hflX"
                     /locus_tag="VFI55_00470"
     CDS             11381..12832
                     /gene="hflX"
                     /locus_tag="VFI55_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET7072542.1"
                     /translation="MTYPEIPPHDQPDGRPVSEPSTGELALEDRSALRRVAGLSTELA
                     DVSEVEYRQLRLERVVLVGVWTEGSATDNQASMEELAALAETAGSEVLEGLIQRRDKP
                     DPSTYIGSGKAQELREVVLATGADTVICDGELSPAQLTALEKAVKVKVIDRTALILDI
                     FAQHATSREGKAQVSLAQMEYMLPRLRGWGESMSRQAGGRAGGSGGGVGLRGPGETKI
                     ETDRRRIRERMSRLRREIKDMKQARDTQRSRRLASDIPSIAIVGYTNAGKSSLLNALT
                     GAGVLVQDALFATLEPTTRRAEFEDGRPFVLTDTVGFVRHLPTQLIEAFRSTLEEVVD
                     ADLLVHVVDGSDVNPLAQINAVRQVISEVFVDYKADDHGDPPPELLVVNKVDAAGDLM
                     LAKLRHVLPGAMFVSARTGDGIDALKRRMSELAAPTDTAVDVVIPYDRGDLVARLHAG
                     GRVQHQEHNADGTRIRARVPVALAASLREFSTR"
     gene            13011..14171
                     /locus_tag="VFI55_00475"
     CDS             13011..14171
                     /locus_tag="VFI55_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7072543.1"
                     /translation="MSGAIKYQRTLFEPEHELFRASYRGFLDRHVAPYHDEWEKAKIV
                     DRGVWLEAGKQGFLGMSVPEEYGGGGNPDFRYNTIIAEETTAGRYSGIGFGVHNDIVA
                     PYLLELATEEQKQRWLPKFCTGEIITAIAMTEPGTGSDLQGIKTRAVKQGDHYVLNGS
                     KTFITNGINSDLVIVVAQTDPDKGAQGFSLLVVERGMEGFERGRHLDKIGLDAQDTAE
                     LSFTDVHVPAENLLGEPGMGFIYLMQNLPQERISIAIMAAAAMEQVLEHTLRYTKDRK
                     AFGRPIGSFQNSRFVLAELATEATVVRVMVDEFVRLHLDKKLTAEQAAMAKWYSTEKQ
                     VHVIDRCLQLHGGYGYMREYPVARAYLDARVQTIYGGTTEIMKEIIGRSLGV"
     gene            14792..16936
                     /locus_tag="VFI55_00480"
     CDS             14792..16936
                     /locus_tag="VFI55_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LGFP repeat-containing protein"
                     /protein_id="HET7072544.1"
                     /translation="MRKLVARALIGMATTVMAAALLAPTVSASPIGDAETAMMAAWEK
                     AGGDTSPLGARKGDVYPVGDGFALDFDSGRMYYTTDTGAKFVFGPILDKYDSLGGPAG
                     SDLGFPTINEVPGLAGPDSRVATFSASDKPVIFWTSEHGAYVVRGAMNDAWDKLGSSG
                     GVLGAPTGDETYDGEVTSQKFSGGDISWNRQTKEFTTNPAVLADQLKGLQVVIDPTAA
                     INMAWRAAGAATGPLGAKQGGTYPIGGDGIAQNFANGKVFFSPATGANALETDILAKY
                     ESLGGPAGSDLGFPTANESDGGITPPSRTAPFSASDKPVIFWTADHGAFVVRGAMKAA
                     WDKLRGPAGKLGAPVGDQAVDGDVVSQQFTGGKISWNRAQNSFTTDPSNLAPLLSGLQ
                     VSGQSQPSTSAMPSHAKKLAWHWWWLLAAIPVLVLVVLAGLMGLGWYRRRSRAHTAAG
                     ETDHDDDGYGVVVDDHLGHDDADLATEHFERGDVHSDQESASDAGPAARVSWSREAAA
                     DDTLPEDVILARVYGEDVPSEFDEGAGREALPGVDNENTDAVDTDSFRAVSEDQLSEA
                     GYSGDAEASAEDESAEGDHAEEHYAEVDHAEEHYAEAAVPHSDSDADAQVEDAVAADA
                     AASDAEPRIGRHAAADAGAVSHLDGEAHVRPTMRLPLDDPYQVPDGYPIKANASFGLY
                     YTPGSPLYHDTLAEFWFASEEVAQANGFVKAD"
     gene            complement(16955..17674)
                     /gene="lexA"
                     /locus_tag="VFI55_00485"
     CDS             complement(16955..17674)
                     /gene="lexA"
                     /locus_tag="VFI55_00485"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HET7072545.1"
                     /translation="MGDSKDTSSAGSSVGSDGRLHSVDSTLTERQRTILNVIRASVTS
                     RGYPPSIREIGDAVGLTSTSSVAHQLRTLERKGYLRRDPNRPRAVDVRGAEDATPAAP
                     AALTDVAGSDALPVPTYVPVLGRIAAGGPILAEEAVEDVFPLPRELVGEGTLFLLKVV
                     GDSMVEAAICDGDWVVVRQQHVADNGDIVAAMIDGEATVKTFKRAGGQVWLMPHNPAF
                     DPIPGNDATVLGKVVTVIRKI"
     gene            17986..18480
                     /locus_tag="VFI55_00490"
     CDS             17986..18480
                     /locus_tag="VFI55_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET7072546.1"
                     /translation="MTIIHTVSPRTGSVRRPINGPAQRIPSGRDGQARSRRPAPSRPG
                     GAPPRYHGTGVAMSVAPHRRRPVPLATTLGLALVAGLITLWLGLVANVGQQVNGESPG
                     SAAQVPDRLAVVRVEPGESLQDVAARVAPDAPARQVADRIRDLNALNSPALVAGQTLI
                     APVG"
     gene            18637..19101
                     /gene="nrdR"
                     /locus_tag="VFI55_00495"
     CDS             18637..19101
                     /gene="nrdR"
                     /locus_tag="VFI55_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HET7072547.1"
                     /translation="MHCPFCRHPDSRVIDSRETDEGQAIRRRRSCPECGRRFTTVETA
                     VLAVVKRSGVTEPFSRETVIRGVRRACQGRQVDEDALNLLAQQVEDTVRAGGSPEVPS
                     HEVGLAILGPLRDLDEVAYLRFASVYRSFSSADDFEREIEAMRAHRKVSTAG"
     gene            19188..19673
                     /locus_tag="VFI55_00500"
     CDS             19188..19673
                     /locus_tag="VFI55_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HET7072548.1"
                     /translation="MSPDLHPDLQALAPLLGTWAGQGDGEYPTIQPFAYLEEVAFSHV
                     GKPFLAYTQKTRAVSDGRPLHAETGYLRVPQPGRVELVLAHPNGITEIEVGTYSVTVD
                     LIEVELSSTAIGLTPTAKEVTALGRSLRIDGDELAYTLRMAAVGQPLQDHLAAVLHRQ
                     R"
     gene            complement(19680..20366)
                     /locus_tag="VFI55_00505"
     CDS             complement(19680..20366)
                     /locus_tag="VFI55_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HET7072549.1"
                     /translation="MGIDVAVVRVFTDSDGNFGNPLGVVDASLVGVADRQRLASQLGY
                     SETIFVDFPAAGSTTAHATIYTPQTELVFAGHPTVGASWWLRENGRPINTLQVPAGIV
                     QVHYEGDLTGVSGRSEWAPELAINEFDSVADLFAADPADFPDDTAHYLWTWIDRAAGS
                     LRTRMFAANLGVPEDEATGSAAMRITDFLSRDLRITQGKGSVIETTWNPEGWVLVAGR
                     VVNDGVRHID"
     gene            complement(20413..>21221)
                     /locus_tag="VFI55_00510"
     CDS             complement(20413..>21221)
                     /locus_tag="VFI55_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7072550.1"
                     /translation="GSSAKPVDGDYSLGSFASVVRDLMMTLGLERGTIVGQSLGGGVA
                     MQFAYQFPHMVERLILVASGGVTKDVNLVLRLASLPMGGEALALLRLPLVLPAIQLAG
                     RLAGLAIGSTGLGRDLPNVLRILDDLPEPTASAAFSRTLRAVVDWRGQIVTMLDRCYL
                     AEGTPVQIVWGTKDVVVPVRHAWMAHAAMPGSLLEIFEGSGHFPFHDDPARFIDIVQR
                     FIDTTQPAQYDQATMRELLRTGGGEKTVTGPADTRVAVLNAMGSDERSAT"
BASE COUNT         3373 a         6971 c         7411 g         3466 t
ORIGIN      
        1 tcggaccgcc gcccgggcga tcgaggacgg cgtcgagccc cgcgacgttt ggcgggcgtt
       61 gtgcgtcgac ttcgacgtgc cccgcgacca gtggtgaggc ggcgttctcg cgccattgag
      121 tgtgcgctgg tggcgtcgag cgtgcgctgg gggctcgatc cgggagccct ttgccaccct
      181 ggatacacat ccaaagccgg gaacgcacac tcaatgtcag cggcgcaggc tcaactccgg
      241 ttcggccgcg gtgtccggcg gaacacgcaa tcgccgcata cggaccttcc ggggacgcga
      301 tagaacaggc agcagctacg acgcctgaag cccagatgcg gggtggtgat caggccggat
      361 ccggccaacg tgcccgtgtc cagtagtgag ttggtgatcg cgacgatggc gcggcgcagg
      421 tcgggtcgcg ccgaaagcaa agctcgggac gctccgacga gtgcggacgc gatgttgccg
      481 gccagcaggg cgtgcgccag cttgaccggc agaccggcgg ccagcggttc caggtgttgt
      541 tgcacgacga tgcggtacag gaagtccgct gaaggcgcgt tgacggcttc tccgacgggt
      601 tcgggcattc gcagttgggc cccgtcgtcg gcacgttgca gctctgtgag atctggaacc
      661 acgccgtggc ccagcgtgca cgcgagcacg ggtgaccaca gccgggtggc atggccgaga
      721 tgtaccagcg aggcgccgat ccgcaagtcc gtcgtgtggt agcgccgggc ggtggcggcg
      781 atcaaatcgg cgaatccgtg gtcgtatgac cgcctgaccg gatgccaacc cctcgcgtcg
      841 ccgcgcacgg taagcgtgaa aaaccctctg taggaagata tctcggccag ctggatagag
      901 atgtccatga gcgcgcgacc accctctctg gggagatccg ccccagtgac gtgagtgaag
      961 cgccgacggt gagtatctgg ctctcgggtc aggacatgcc caacccgatc acagtggcgg
     1021 gaccgcaccg gattcgcacc ggtttcctca gcttcttgta gagctttgtg tagagctttt
     1081 gtagagcttt gtgcaggccc gttggcgccc tttcggtagc cgatattgcc acacggaacc
     1141 agcacggcgt gtcaaacttg aatcgaacag atgttcgtct actgtggtga tcattcgaag
     1201 aggacgactt gtcggtggcc ttctctagtg tcacggccaa ccgaccgata ccggtcacac
     1261 cgacgatagg aagaggcacc atggcgcagg cccccgaccg cgagaaggct ctcgaattgg
     1321 cgatggccca gatcgaaaag aactacggca aaggctcggt gatgcgtctc ggcgacgaga
     1381 tgcgtcagcc gatctcagtc atcccaaccg gatccatcgc gctggatgtg gccttgggca
     1441 tcggcgggct gccccgtggc agggttgtgg agatctacgg cccggagtcc tcgggtaaga
     1501 ccaccgtcgc gctgcacgcg gtggctaacg cccaggccgc cggcggtgtc gcggcgttca
     1561 tcgacgctga gcacgccctg gaccccgatt acgccaagaa gctcggcgtc gacaccgatt
     1621 cactgctggt cagccaaccc gacaccggtg aacaggcgct ggagatcgcc gacatgctga
     1681 tccgctccgg cgcactggac atcgtggtca tcgactcggt ggcggccctg gtgccgcgcg
     1741 cggagctcga aggtgagatg ggggacagcc atgtcggcct gcaggcccgg ctgatgagcc
     1801 aggcgctgcg gaaaatgacc ggtgcgctca acaattcggg gaccacggca atcttcatca
     1861 accagctccg cgaaaagatc ggggtgatgt tcggcagccc cgagaccacc acgggtggaa
     1921 aggccttgaa gttctacgcg tcggtgcgct tggacgtgcg ccgaatcgag acgctcaagg
     1981 acggcaccaa tgcggtcggc aaccgcaccc gggtcaaggt cgtcaagaac aaggtgtcgc
     2041 cgccgttcaa gcaggccgaa ttcgacatcc tctacggcaa gggcatcagc aaggaaggct
     2101 cgctgatcga tatgggtgtg gaccagggct tcatccgcaa gtccggttcc tggttcacct
     2161 acgagggtga gcagctcggc cagggcaagg agaatgcccg caacttcttg atggaaaacg
     2221 gcgacgtggc caacgagatc gagaaaaaga tcaaggaaaa gcttggcatt ggcgcggtcg
     2281 tgaccgatga cgacatcctg cccgcccccg tcgatttctg agccgtcccg cgaagagcag
     2341 gcgcgggcgt tgtgcctgcg cctgctcacc gcgcgggctc gcacgcgggc cgagttgtcc
     2401 ggccagctgg cgaaacgtgg ataccccgac gacgtcacgg acagggtgct cgcccggctg
     2461 gccgccgtcg gactggtgga cgacgtcgaa ttcgccgaac aatgggtcga gtctcgccgc
     2521 tccagcgcgg gaaaaggcag gcgtgcgctg gccgccgagc tacacaccaa gggcgtcgac
     2581 aacgaggtga tcaccacggt gctcggcggg atcgacaccg gcgctgagcg gcagcgggcc
     2641 gagcagctgg tgcgggccag actcaaacgc gaagatctcg acggtgactc gacgcgagtg
     2701 gcccgccggt tggtcgggat gctggcgcga cggggctacg accagaacat ggcctacgac
     2761 gtggtgcggg ttcagctaaa cgtcgagctg gagcgccgac gtacgtgagg tgacgagctg
     2821 agccgcgggg acaagcccgg acgcaccaac gccgtaccat ggccccgtga cttcgatggt
     2881 gacgcaagac gccgcagccc gggctgcggc gccggcgcgc acctaccaag tccgcaccta
     2941 cggctgccag atgaacgtcc acgactccga gcggttggcg ggcctgctgg aagccgccgg
     3001 ctaccggcgg gctgccgacg gggccgacgc cgatgtggtg gtgttcaaca cctgcgccgt
     3061 ccgcgagaac gccgacaaca agctgtacgg caacctcagc cacctggccc cacgcaagcg
     3121 cggcaatccc gacatgcaaa tcgcggtcgg tggctgcctg gcgcaaaagg accgcgacgc
     3181 gctgctgcgc aaggcgccgt gggtcgatgt catcttcggc actcacaaca tcgggtcgct
     3241 gcccacgctg ctcgaccggg cgcggcacaa caaagtcgcg caggtggaaa tcgccgaggc
     3301 gctgcagcag ttcccgtcct cgctgccgag cgcccgtgaa tccgcgtatg ccgcctgggt
     3361 ttccgtctcc gtcggctgca acaacagctg caccttctgc atcgtgccct cgctgcgcgg
     3421 caaggaggtc gaccgcagtc cggccgacat cctggccgaa gtgcagtcgc tggtggacga
     3481 cggcgtgctc gaagtgacgc tgctgggcca gaacgtcaac gcctacgggg tttccttcgc
     3541 cgacccggcg ctgccccgcg atcgcggcgc cttcgccgag ctgctgcggg cctgcggccg
     3601 ggtcgacggg ctggaacggg tgcggttcac ctcgccgcat ccggccgagt tcaccgacga
     3661 tgtcatcgag gcgatggcgc agacgccgaa cgtctgcccg gccctgcaca tgccgctgca
     3721 gtcgggatcg gaccgggtgc tgcgcgcgat gcggcggtcc taccgcgccg agcgctatct
     3781 gggcatcatc gaccgggtcc gggcggccat gccgcacgcg gccatcacca ccgacttgat
     3841 cgtcggattt cccggtgaga ccgaagagga cttcgccgcg accctcgacg tggtgcggca
     3901 ggcccggttc gcggccgcgt tcaccttcca gtactccaag cggccgggca cccctgccgc
     3961 cacgttcgac ggccagctgc ccaaagccgt tgtgcaggaa cgctacgagc ggctggtcga
     4021 gctgcaggag cggatctcgc tggagggcaa ccgcgcgctg atcgggcaga ccgtcgagct
     4081 gctggtcgcc gccggcgaag gacgcaagga cagccgcacg gcgcgcatga gcgggcgggc
     4141 acgcgacggc cggctggtgc acttcaccgc cgacggcagg gtccggccgg gtgacatcgt
     4201 caccacggtg gtcaccgagg ccgccccgca ccacctcatc gcggacgcgg gcatccgcac
     4261 gcaccgcggc acccgggcgg gtgacgcgca cgccgccggg cagcgcccgg gcagcatcgg
     4321 cctcggcatg cccacggtcg gaccgtccgc cggcccttca gaatccacac catccctggg
     4381 atgtgcccga tgaagaaaga cgagacggac ctcgacgctt tgcgaaccgc aatcgacgcc
     4441 gcggaacgcc gggtggcggg tgagatcgag cccggctcaa gggctctcgt ggtcgcaatc
     4501 ctggtgttcg tgctgctggg ttcgttcatc ttgccgcaca ccggcgatgt gcgcggatgg
     4561 gatgtcctgt tcggcagcca cggggcgagc gccgccgcgg tctccctgcc gtcgcgggtg
     4621 ttcgtctggt tggtgctggt gttcggcgtc ggcttctcga tgttggcgct ggtcacccgg
     4681 cgctgggcag tcgcctggct cgcactggcc ggaacgtcga tcgccagcgc gatcgggttg
     4741 ttggccgtct ggtcgcggca gacctccccc ggccatcccg gacccggggt gggactgatc
     4801 gtcgcgtgga tcaccgtgat cgtcttgacg tttcactggg cccgggtggt gtggtcgcgc
     4861 accgccgtgc aactcgcggc cgaggagcag cggcgccagg ttgccgcaca gcagcagtca
     4921 aagaccctgc tggacacctt cggaaccgac accaccagcc ccggcgagcc ggacaacccc
     4981 ggcgacccgg acgccggcag gcctgcaggt ccgtaacgac tagcgcctca gcgcctcggc
     5041 ggccgcgtcg gcccactgcc gccactgctc ggcgttggct ttggcctgtt cggcgtcctt
     5101 ggtccggccg gccgctgccg cctttcgggc ctgctgttcg tattgctcgg cgcgagtccg
     5161 gaactgctcg gcccgagcct gcgcctgagg atcggaccaa tccgtctcgt cggcttcgcg
     5221 caccttcttc tcgaccgcac gcagccgccg ctccaagtcg gcggaccgtt cccgcggcac
     5281 cctcccgatc gcatcccact tctcgccgat ggaccgcagc gcggcccgag cggcgtcgcg
     5341 gttgctggta tcgatgcttt ccgcctccgt cagcaacgcc tctttggcgg tcgcgttcgc
     5401 gtgcaactcc gcgtcctttt ccgccgtcac ggcgttgcgg gccgcaaaga acgcgtcctg
     5461 ggcggccttg aagcggcgcc agagggcatc gtcgacgtct ttgctcgctc gtcccgccgc
     5521 cttccactcg gtgagcagct tgcggaattc cgtgctggtg gcggtccagt ccgtcgaggc
     5581 ggacagctct tcggcgcgct cgcagagctc ctccttcgcc tgacggatcc ccgatcgctc
     5641 ccggtccagc tcggcgaaat gggatccgcg ccgccggttg aaggtttccc gggcagccgc
     5701 gtagcgcttc cacaacgcct cgtcgacttt tcggtccagg ccggtgatgg tcttccactc
     5761 gtcgaggatc gcgcgcagcc ggtcaccggc gactttccat tgggtcgagt tggcggccaa
     5821 gtcctccgcc tcggcggcca gcgcctcctt gcgggaaacc agcgcggccc gctgcccctc
     5881 acggcgcgag cgatccgcgg acacgatgac gtcgactcgc gcaatcaggt tcgtcaaccg
     5941 gccctcgagc gcgtcgacat cgcccagcac ggatgccgtc ggcaacgtct cggccagcgc
     6001 ggaggcattg gccctgatct tgcgggcgtc tccggtgccc gacgtcagcc gctcctccat
     6061 gagcgtgacc tcggtgctca gatcgtcgaa cctgcgcccg aagtgggcga atgcggcttc
     6121 cgggtcgccg gcctgccagg agccgacgac acgctcgccg gccgaactga tcagccatac
     6181 cgtgccgtca tcgtcgacgc ggccgaactg gtgcggattg ctcggcgggt gcgttgccac
     6241 cgcgtgggtg gtgggccgcg gccccgagct cagaggccgg gggcccggac gcggtcccgg
     6301 acgtggcgcc ggccggggcg aatcgttgct gatgggcttg tcagccgtca tgccgctcgt
     6361 caccaccctt ttccaatcct ttcgcgcggt ctgtgtcccg cgcacccgcc gcgctgagcg
     6421 gcacccgtac ggtcgggagc gtttggcgcg gcctcgccgc tgtcgcctgc tgctccgcaa
     6481 ccggttccaa cagtattcaa gcagcttggc gagcggcgcg cgcccacctt tgccgctgtt
     6541 agtgccccgg ccggttaccg gccgccccgc cgggccccgc aaatgccgtc gcgggcgatc
     6601 gcgctggatc gataacagtt cgattccacc gtcgctcggg acatatggcg gcatcgccac
     6661 gcccggtacc gtgacgaggc gagatgttac ggctgttacg ggtgggatcg gagggggaga
     6721 tcaccatagc caaggtggat atcgcggcgc tgctcgcgct ggtcgcggcg ctgatatcgg
     6781 gcgtcggaga cgtggtccgt cagcggtccg cacacgaaat caccgaggaa aaagtcggcc
     6841 atgtcgagct gctgcgcatg tcgttgcgcg acacgcggtg gtggctgggc ggcggggcgg
     6901 cagtgctcag tgccgcgctg caggccgctg cgctgggact cggctcagta gtgttggtgc
     6961 aggcgctgca ggtgacggcg ttgctgttcg ctttgccggt gtcagcctgg gtgaccaaaa
     7021 aggcgctgac ccggcgggag tgggggtggg cggtactgct ggccaccgcg gtggcggtgt
     7081 tcgtgatggt cggcgaaccg gccgccggct accagcgggc atcgctgcaa acctgggcga
     7141 tcgtggccgt gctgatcgcc ccggtgatgg cgttttgtgt gctgggcgcg cgcacctgct
     7201 cgggcccgcc ggccgcggtg ctgctggcag tggtgtcggc gtcgtcgtgg gcgttgttcg
     7261 cggtgttcac caaggcgatc gtcgacgtgg cggacggcgg tctgtgggcg ttgctgcgca
     7321 ctcccgagct gtacggctgg ctgctggtcg cggcactcgg gacggtgttt cagcagtcgt
     7381 cgtttcgggc cagctcgctg acggcctcgc tgccgacgat gacggtgacc gagccggtgg
     7441 tggcttcggt gctgggtgtc accgtactgg gcgaaacact cggcgccgac ggcccgaagc
     7501 tcatcgtgtt ggtggcctcg gtggtggtgg tgattgtcgc gaccgcggca ctggcccgtg
     7561 gcgaggcggt ttctttggcg tccggagacg aggggcaact cagggatcgc cccgagccgg
     7621 aggcggctgc ttcgcccgtc gtggtgggcg caaccagcca cgcatagccg cccgacgcgg
     7681 gtcaagaccc tggcgccgcc gcactacagt ggtcgttgaa ctgtgatcga acagtgcgtg
     7741 tacgtacagc ttcgaaggga acgctggtgg gaacgctcgt gagggggtcc ctatgtcgaa
     7801 agtggatgtc gcggcgctga tcgccttgtg cgcggcgctg gcctccgcga tcggcgatgt
     7861 gatccggcag cgctccgccc aggagatcac ggacaaggaa gtcggtcacc tcgagctgtt
     7921 ccggatgtcg ctgcgcgacc gtcggtggtg gttgggcggc ctggcggcga tcaccaatta
     7981 cagcctgcag gcggcggccc tggcctgggg gtcggtggtg atggtgacgt cactgcaggt
     8041 ggccgcgctg ctgttcgccc tgccgatcta cgcgcggctg agccataacc gggtgacccg
     8101 ctgggagtgg atctgggcgg tggtgctggc ctgcgcgttg gcagtggtga tcatcgtcgg
     8161 cgacccggag gccgggcagc agcgggcgtc gctgtcgacg tggctcgtgg tggccctggt
     8221 gatggtcccc gcgttggtgt tgtgtgtgct ggcggcgcgg atctggtccg gccgtccgat
     8281 cgcggccgtg ctgctggcgg tggtcgcggg gtcgtcgctg gcgctgttcg cggtgctgac
     8341 caagggcgtc gtcgaggtgg gcgaagaagg cctgggtgcg gtgctgcgcg cgcccgagtt
     8401 ctatccctgg ttgctggtgg cactgtgcgg aatgatcttc cagcagtcgg cgttccgtgc
     8461 cggcgcgctg acggcgtcgt tgccgacgat gaccgtggcc aagcccgtgg tcgccggtgt
     8521 gctgggtgtt accgtgctcg gcgagacgct cggagcgaca ggtcccgcgg cgtttgtgct
     8581 ggtcgcggcg gttgccatgg tcatcatcgc gacggttgcg ctggcccgcg gcgaggccgc
     8641 ctcgatggcg ccccgaaccc gtcgcgaccg caagggcagc tcctcccagg aaacccccct
     8701 cgaagccccc ctttgaccaa aggcgagccg aaccatcact tggtccggcc cggctcccgc
     8761 tctcgtgact gtccgagccg ccaagttagt ttggtgagca tgctgagcgc catcgcgatc
     8821 attccctccg cgccggtgct cgtccccgaa cttgccgggg ctgccgccgc cgaagtcgcg
     8881 gacctgaccg cggcggtgaa ggcagccgcg gccttgctgc caccacgctg ggtggctgtc
     8941 ggaacgggcc gagccgacga ggtagtgggc cccagcgcca cgggcacctt tggcggcttc
     9001 ggggttgacc tgccggttcg gctctccgcg cgagacgacg gcctcccgat cgaattcccc
     9061 gtgtgcgcgc tgctggccgc gtgggtgcgc ggccaggtca ggcccgacgc cgatgcacag
     9121 gtgcgggtct accgaggcga ccaggacgcc gaaaccgcgc tggcccgcgg cagggagctg
     9181 cgcgccgaga tcgaccgggc gccggacccc gtcggggtgt tggtcgtggc cgacggcgcg
     9241 aacaccctgt caccggccgc gcccggcggc tatgacccgc gcaacgccga gtcgcaactg
     9301 gtgctggacg acgcgctggc gagcggcgac gtcaccgcgc tggcccggct cccgcaccag
     9361 atcgtggggc gggtcgcgtt tcaggtgttg gccggtctgg ccgagccggg cccgcgatcg
     9421 tcaaaggagc tctaccgggg agcgccctac ggcgtgggct acttcgccgg catttggcag
     9481 ctatgaaatc aaccgggcga ccgctcgcga ttgtcgggcc gaccggcacg ggcaagtccc
     9541 aactggcgct ggactttgcc gaacgcgtcg cgcaactcgg cggcgaggtg ggtgcggaaa
     9601 tcgtcaacgc cgacgccatg cagctctatc gcgggatgga catcggcacc gccaagctgc
     9661 cggtcgagca gcgtcgtggc attccgcatc atcaactcga cgtcctgaac gtcaccgaga
     9721 ccgccaccgt cgcccgctat cagcgggacg ccgccgcgga catcgaagcg atcgcggccc
     9781 ggggtgcggt gccggtgatc gtgggcggtt cgatgctcta catccaatcg ctgctcgacg
     9841 actggtcctt tcccgcgacc gatcccgcga tccgggcgcg ctgggagcag cggctcgccg
     9901 aggtcggagt gggccggctg cacgccgagc tggcccgccg cgacccggga gccgcggcgg
     9961 cgatcctgcc caccgacgga cggcgcgtgg tgcgggccct tgaggtcgtc gaactcaccg
    10021 ggcagccgtt cgccgcgtcc gcgccacgca tcggcgcgcc gcgctgggac accgtcatcg
    10081 tcgggttgga ctgtgacaca gcgattctcg atgagaggtt ggcgcgccgc accgacacga
    10141 tgttcgatca gggcctggtg gacgaggtgc gcgcactgct gcgcgagggc ctccgcgacg
    10201 gggtcaccgc cgcacgcgcc ctgggctacg cccaagtgct ggcggcgctg gatgccggcg
    10261 ctgaagcagg cccaagtgcg gttgcgctgc gcgacgcgcg ggagcaaaca tacgtgggca
    10321 cccggcgtta cgtccgccgt cagcggtcct ggttccgtcg cgaccaccgg gtgcactggc
    10381 tcgacgccgc cgaccgtgcc ggcctcgtcg acggcgcgtt gcgggcgtgg cggcacgtat
    10441 cctgaacagg tggaattcgc caagggccac ggcacccaga acgacttcgt gctgctgccc
    10501 gacctcgacg ccgagctgac gctgaccgcg gcgcgggtgg cggcggtgtg cgaccgacgc
    10561 cgcggattgg gcgccgatgg cgtgctgcgg attaccaccg cgggcgtcgc cctggcggtt
    10621 ggcgtgctct cacgcctgcc cgacggcgtc ggcgtgacgg actggtacat ggactaccgc
    10681 aacgccgacg ggtcgaccgc gcagatgtgt ggcaacggcg tccgggtgtt cgcgcactac
    10741 ctgcgggcca gcggtttgga ggcccgcgac gagttcgtgg tcgggtcgct ggccggcccg
    10801 cggccggtca ctgtgcatca cgtcgacgac accaacgccg aagtcaccgt tgacatgggc
    10861 aaggccaacc ggctcggcac gggcgacgcc gtcctcggcg gccggcggtt taccggcctg
    10921 gcggtcgatg tgggcaaccc gcacctggcc tgcctggatc cgcggctgag tgccgaggaa
    10981 ctcgcggcgc tggacgtggg cgcgccggtg cagttcgacc gtgcccagtt tcccgacggc
    11041 gtcaacgtgg aagtgctcac gtcgccggtc tggggcccgg ccggcggggc ggtccagatg
    11101 agggtccacg agcgcggggt gggcgagacc cgctcgtgcg gtaccggaac ggtggccgcc
    11161 gcggtcgccg cgctggccga cgccggtgcc gacaccggga cgcttaccgt gcgggtgccc
    11221 ggcggtgacg tcgtggtcac catcaccgag gccaccagct acttgcgagg gccgtcggtg
    11281 ctggtggccc gcggccagat cagcgaggaa tggtggcgta cgcaacagcg ttaattcgcg
    11341 tgcacgacgc cgatgtggcg tgcactattg ctaattgcct atgacatatc ccgagattcc
    11401 cccacacgat cagccggatg gtcgtcccgt ttctgagccc agtaccgggg aacttgccct
    11461 cgaagaccga tcggcgctgc gccgggtcgc ggggctgtcc accgagctgg ccgacgtctc
    11521 cgaggtcgag taccgccagc tgcggctgga gcgcgtcgtg ctggtggggg tgtggaccga
    11581 gggcagcgcc accgacaacc aggccagcat ggaggagttg gcggccctgg ccgaaaccgc
    11641 cggctcggag gtgctcgagg gacttatcca gcgccgcgac aagcctgacc cgtcgaccta
    11701 catcggctcg ggcaaggcgc aggaattgcg ggaagtggtg ctggcgaccg gtgccgacac
    11761 cgtcatctgc gacggcgagc tgtccccggc ccagctgacc gcgctggaga aggcggtgaa
    11821 ggtcaaggtc atcgaccgca ccgcgctgat actggacatt ttcgcccagc atgccaccag
    11881 ccgggagggc aaggctcagg tgtcgctggc ccagatggag tacatgttgc cgcggctgcg
    11941 cggctggggt gagtcgatgt cgcggcaggc cggcggccgc gcgggcggca gcggcggggg
    12001 agtgggcctg cgcggtcccg gtgagaccaa gatcgagacc gaccgccgtc gcatccgcga
    12061 gcggatgtcc aggctgcgcc gcgagatcaa ggacatgaag caggcccgcg acacccagcg
    12121 cagccgccgg ctggcgagcg atatcccgtc gatcgccatc gtgggctaca ccaacgcggg
    12181 caaatccagc ctgctcaatg cgctgaccgg agccggggtg ctggtgcagg acgcgttgtt
    12241 cgccactctg gaacccacca cccggcgcgc ggaattcgag gacggccggc cgttcgtgct
    12301 caccgacacc gtcggattcg tccggcacct gcccacccag ctgatcgagg cattccgctc
    12361 cacattggag gaggtcgtcg acgcggattt gttggtgcac gtggtggacg gctcggacgt
    12421 caacccgctg gcccagatca atgcggtgcg ccaggtgatc tccgaagtct tcgtcgacta
    12481 caaggccgac gatcatggcg atccaccgcc cgaactgctc gtggtcaaca aggtcgacgc
    12541 cgccggcgac ctcatgctgg ccaagctccg ccacgtcctg cccggcgcga tgttcgtctc
    12601 ggcgcgcacc ggcgatggca tcgacgcctt gaagcggcgg atgtccgagc ttgccgcccc
    12661 gaccgatacc gccgtggacg tggtgatccc gtatgaccgc ggcgacctgg tggcccgcct
    12721 gcacgccggt gggcgcgtgc agcaccagga gcacaacgcc gacggcaccc ggatcagggc
    12781 ccgagtcccg gtggcgctgg ccgccagtct ccgggagttc tccacccggt aaccgccgcg
    12841 agcgtcacgt ggccgcgatt ttcgccgggc ggtttcgcag tggcgttacg cccggcgcgg
    12901 ccccggggac cgtcgtaccg acccgcacct ggccagctgc cgaccaaccg tctggttggt
    12961 atatcttggg cgaagagttc ccgttcgccg gcggtcagcg gaggttatcc atgagtggtg
    13021 ccatcaagta ccagcgcact cttttcgaac ccgagcatga attgttccgt gcgtcctacc
    13081 ggggctttct ggatcgccac gtggcgccct atcacgacga atgggaaaag gcgaagatcg
    13141 tcgaccgcgg cgtctggctc gaggccggca agcagggctt cctgggcatg tcggtgcccg
    13201 aagaatatgg cgggggaggt aatcccgact tccgctacaa cacgatcatc gccgaggaaa
    13261 ccaccgccgg gcgctacagc ggcattggtt ttggcgtgca caacgacatc gtcgcgccat
    13321 atctgctgga gctggccacc gaggagcaga agcagcgctg gctgcccaag ttctgcaccg
    13381 gcgaaatcat caccgcgatc gcgatgaccg aacccggaac cggcagcgac ttgcagggca
    13441 tcaagacgcg cgccgtcaaa cagggcgatc actacgtgct caacgggtca aagacgttca
    13501 tcaccaacgg aatcaactcc gacctggtga tcgtggtcgc gcagaccgac ccggacaagg
    13561 gcgcgcaagg cttttcgctg ctcgtcgtcg aacgcggcat ggagggcttc gagcgcggcc
    13621 gccatttgga caagatcggt ctggacgccc aggacaccgc cgagctgtcg ttcaccgacg
    13681 tgcatgttcc ggccgaaaac ctgctgggcg aaccgggtat ggggttcatc tacctcatgc
    13741 agaacctgcc gcaggaacgg atttcgatcg ccatcatggc ggccgcggcg atggaacagg
    13801 tgctggaaca cacactgcga tacaccaagg accgcaaagc ttttggcagg ccgatcggca
    13861 gcttccagaa cagccgtttc gtgcttgccg aacttgccac cgaggccacc gtggtgcgcg
    13921 taatggtcga cgagttcgtc cgcctgcacc tggacaaaaa gctgacggcg gaacaagccg
    13981 caatggccaa gtggtattcc accgaaaagc aggtgcacgt gatcgaccga tgtctgcaac
    14041 tgcacggtgg ctacggttac atgcgcgaat acccggtcgc ccgagcgtat ctggatgctc
    14101 gggtgcagac gatttacggc ggcacgaccg agatcatgaa ggagatcatc ggccgcagcc
    14161 tcggggtcta gcccgtcggt gctgtccagg cggttcgtgt cctgccgcaa tcgcgtgcga
    14221 cgacgcattg atagcgacca gaacgaccaa caagcggcca cccttgcggg ccgtcgccgc
    14281 ttagctggcc cgcaataatt gaacggtcta tttcgttttt tggcgggtag atcgtgaacc
    14341 gactaacggc ttcgcgtacc gtcggcgcat gcgttttgcg tatgtcgact gtgtagccgt
    14401 tgatcgtcgg ggcgatcacg acgccggtaa ctgcccggcc gggtctggag ccattaactt
    14461 cgtgcccggc ccgcgagtgg tgcaccaggc ttggggaacg ttagcccgaa taccgcgcgt
    14521 gcgaaggcac ggttggtccc gatacgggcg tggaacggca tcatttcgac atcggcgcgg
    14581 tctccgtttc cgtaatattc gtgatttgcc ttcgcatttt tggtcgacgg gggcaaagtt
    14641 atgcgagccc ggcgataatg cgcgttgtaa tggggttacc gccccgcaat gggcgtacga
    14701 cccgctcgca gggtctgcag ggtgcgggca tggccacagc taggggccta tccgtcggct
    14761 gggaggcttt gtgaaggggc agagaggtcc aatgcgcaag ctggttgcgc gcgcgctgat
    14821 cggcatggcg acgacggtga tggccgccgc gctgctggcg cccactgttt ccgcgtcgcc
    14881 gataggtgac gccgagaccg cgatgatggc cgcgtgggag aaggccggtg gcgacacctc
    14941 gccgctcggg gcacggaagg gcgacgttta ccccgtcggc gacggattcg ccctggactt
    15001 cgacagcggc aggatgtact acaccacgga cacgggcgcc aaattcgttt tcgggccgat
    15061 cctggacaaa tacgactcgc tgggcggccc ggccggcagc gatctcggat tcccgaccat
    15121 caacgaagtt cccggccttg ccggaccgga cagccgagtc gctaccttct ccgcgagcga
    15181 caagccggtg atcttctgga catccgagca cggcgcgtac gtcgtgcgcg gggcgatgaa
    15241 cgacgcgtgg gacaaactgg gcagctcggg cggtgttctg ggtgccccga ccggggatga
    15301 aacctacgac ggcgaggtca cctcgcagaa gttcagcggc ggcgatatct cctggaaccg
    15361 gcaaaccaag gagttcacca ccaaccccgc ggtgctggcc gaccaactca agggtctgca
    15421 ggtagtgatc gatccgaccg cggccatcaa catggcctgg cgcgcggccg gcgccgcgac
    15481 cggtccgctg ggggccaagc agggtgggac atatcccatc ggcggtgatg gaatcgccca
    15541 gaacttcgcc aacggcaagg tgttcttcag cccggccacc ggcgccaacg ccctcgagac
    15601 cgacatcttg gccaagtacg agtcgctggg cgggccggcc ggcagcgacc tgggttttcc
    15661 caccgccaac gagtccgacg gcggcattac gcctccgagc cggactgccc cgttctccgc
    15721 atccgacaag ccggtgatct tctggaccgc tgaccacggc gccttcgtcg tacgcggcgc
    15781 gatgaaggcc gcgtgggaca aactccgtgg tcctgccggc aagctcggcg ccccggtcgg
    15841 tgatcaggcg gtggacggcg acgtggtctc gcagcagttc accggcggca agatttcatg
    15901 gaaccgggcg caaaactcgt tcaccaccga tccatcgaac ctggctccct tgttgtctgg
    15961 cctgcaagtg tccgggcaaa gccaaccgag cacgtcggcg atgccctcgc acgccaagaa
    16021 gctcgcgtgg cattggtggt ggctgctggc ggcaattccg gtgctggtct tggtcgtgtt
    16081 ggcggggttg atggggctcg gatggtatcg gcgccgctct cgcgctcaca ccgcggccgg
    16141 cgagactgac cacgatgacg acggctacgg tgtcgtggtc gacgaccacc tggggcacga
    16201 cgacgccgat ctcgccaccg agcacttcga gcgcggcgac gttcactcgg accaggagtc
    16261 ggcgtccgac gccggtcccg cggcgcgcgt gagctggagc cgtgaagccg cggctgacga
    16321 cacccttccc gaggatgtca tactcgccag ggtctacggc gaggacgtgc cgtccgaatt
    16381 cgacgagggc gcgggacgtg aggcgctgcc gggggtcgac aacgaaaaca cagacgcggt
    16441 ggacaccgac tccttccgcg ccgtctcgga ggaccagctg tcggaggccg gctattcggg
    16501 cgacgcggag gcgtccgcgg aagacgaatc cgcagagggc gaccacgcgg aggagcacta
    16561 tgcggaggtc gaccacgcgg aggagcacta cgccgaggcc gcagttccgc attcggattc
    16621 ggatgcggac gcccaggttg aagacgccgt cgctgcggat gcagcggcgt cggacgcgga
    16681 gccgcgaatc gggcgccacg cggccgcgga tgccggagcc gtgtcgcact tggacgggga
    16741 agcgcacgtg cgcccgacga tgcggctgcc gctggacgac ccgtaccagg tgcccgacgg
    16801 atacccgatc aaggccaacg ccagcttcgg cctgtactac acgcccggca gccccctcta
    16861 ccacgacacg ctggccgaat tctggttcgc cagcgaagag gtcgcccaag ccaacggctt
    16921 cgtcaaggcc gactgaggcg acgcgagccg ggcgctatat cttgcggatc accgtgacga
    16981 ccttgccgag cacggttgcg tcgttgcccg gtatgggatc gaacgccggg ttgtgcggca
    17041 tcaaccacac ctgaccgccc gcgcgcttga acgtcttgac ggtggcctcg ccgtcgatca
    17101 tggccgcgac gatgtcaccg ttgtcggcga cgtgttgctg ccgcacgacc acccagtcgc
    17161 catcgcagat ggcggcctcg accatggagt cacccaccac cttgagcagg aagagcgtgc
    17221 cctcgccgac cagctcacgc ggcagcggga agacgtcttc gacggcttcc tcggcaagga
    17281 tcggtccacc ggccgcgatg cgcccgagca cggggacata ggtgggtacc ggcagggcgt
    17341 cggatcccgc aacgtcggtg agcgctgcgg gcgcggcggg tgtggcgtcc tcggcgcccc
    17401 ggacatcgac ggccctgggg cggttggggt cgcggcgcag gtaacccttt cgctccaggg
    17461 tgcgtagttg gtgagccact gaggatgtcg acgtcaaacc gaccgcgtcg ccgatttccc
    17521 tgatgctcgg ggggtaaccg cggctggtga ccgacgcccg gatgacgttg agaatggtgc
    17581 gctgccgttc ggtcagcgtt gaatccacgg agtgcaatcg gccgtctgag ccaacggatg
    17641 agccggcgga tgaggtgtct ttgctgtcgc ccatgcagtg aatgtagccg ccccgtggcc
    17701 gagaatcaaa catgtgttcg acttgcgtgt cgtgccggca ctggggcctt gagggcactg
    17761 cacggcgcct ggacggcgcc tggacgccgc cgaataacaa acgtgtaact ccccgcgaga
    17821 tcgggtactt gagctccatg gcagtggtca caccgacgga tgtgtcggtg gtgtggtcta
    17881 gcgtttcgct cgttcgacac gcttcgctca aatgttcggc tatcgaacac acaagcgatt
    17941 atagtcgaac acatgagcga gaatcagttg accggaggag cgcagatgac aatcatccac
    18001 acagtctcgc cgcggaccgg tagcgttcgg cgtccgatca acggaccggc gcagcgcatt
    18061 ccgtcaggcc gtgacgggca ggcccgttcc cgcaggcccg ccccgtcgag gccggggggc
    18121 gcgccgccgc gttaccacgg caccggcgtc gcgatgtccg tcgcacctca tcgcaggcgt
    18181 cccgtcccgt tggcgacgac gttggggctg gctctggtcg ccgggctgat cacgctgtgg
    18241 ctgggtctgg tcgccaacgt cgggcagcag gtcaacggcg agtcgccggg atcggccgct
    18301 caggtaccgg accggctcgc cgtggtgcgg gtggaacccg gggagtccct ccaggatgtc
    18361 gccgcccggg tggcgcccga tgcgccggct cgtcaggtcg ccgatcgcat ccgcgatctc
    18421 aacgccctga actcgccggc gctggtcgcg gggcagaccc tgatagcacc ggtcggctga
    18481 cgcggtgaat cgcgaaaacc ttgccatgcc aaggctgctg ggttctggcg gtgccgggcg
    18541 tgtgagtgac gagtccgcat tcgctccgcg ggcgagcgca caggtaggct cggagtgttc
    18601 tgcgatcctg catctcagca cggcgaagga gcggtcatgc actgtccgtt ctgccgtcat
    18661 cccgattccc gggtgatcga ttcgcgggaa accgatgaag gacaggcgat tcggcgccgc
    18721 cggtcatgcc ccgaatgcgg acggcggttc accaccgtgg agaccgcggt gttggcggtg
    18781 gtcaaacgca gcggcgtcac cgaacctttc agcagggaga cggtcatcag gggggtccgc
    18841 cgggcctgcc agggccgtca ggtcgatgag gatgcgctga atctgctggc ccagcaagtc
    18901 gaagacacgg tgcgtgccgg ggggtcgccg gaggtcccga gtcatgaggt cggcctggcc
    18961 atcctggggc cgttgcgcga tctcgacgag gtggcgtacc tccggttcgc ctcggtgtac
    19021 cggtcgttct cttccgcgga cgatttcgag cgcgagatcg aggcaatgcg cgcacaccgc
    19081 aaggtatcga cggcgggctg accgcccgct tgcccgctgc gcggcggcga ggcaccccca
    19141 ccgcgggcgg tgccggcctg catcgtcgtc gagttaggct cgttttcatg tcgcccgacc
    19201 tgcaccccga tctccaggcg ctggccccat tgctcgggac gtgggccggt cagggcgacg
    19261 gcgaataccc gacgatccag ccattcgcgt atctcgagga agtcgcgttc tcgcatgtgg
    19321 gcaaaccgtt tctggcctat acgcaaaaga ccagggcggt gtccgacggc agaccgttgc
    19381 acgccgagac gggatacctg cgcgtgccgc agccgggtcg cgtcgaactg gtgctggccc
    19441 accccaacgg catcaccgaa atcgaagtcg gaacatattc ggtgaccgtc gatcttatcg
    19501 aagtcgagct gtccagcacc gcgatagggt tgaccccgac cgcgaaagag gtgacggcac
    19561 ttggccgttc cttgcgcatc gacggcgacg agctcgcgta cacgctgcgg atggccgcgg
    19621 tggggcaacc gctgcaggac cacctcgccg cggtgttgca ccgacagcgg tgaaccgcgt
    19681 cagtcgatgt gcctgacgcc gtcgttgacg acgcgcccgg ctaccagcac ccagccctcg
    19741 gggttccagg tggtttcgat caccgacccc ttgccctggg tgatgcgcag gtcgcggctg
    19801 aggaagtcgg tgatccgcat cgccgccgac ccggtggcct cgtcctccgg cacgcccagg
    19861 ttggccgcga acattcgggt acgcaacgat ccggcggccc gatcgatcca ggtccacagg
    19921 tagtgcgcgg tgtcgtcggg aaaatcggcg gggtcggcgg cgaaaaggtc agcgaccgaa
    19981 tcgaattcgt tgatggccaa ttccggggcc cattccgagc ggccgctgac gcccgtcagg
    20041 tcgccctcgt aatgcacctg cacgatgccg gccggcacct gaagcgtgtt gatcggccgg
    20101 ccgttctcgc gcagccacca cgacgcgccg acggtcgggt gcccggcgaa caccagttcg
    20161 gtctgcgggg tgtagatggt ggcgtgcgcg gtcgttgatc cggcggccgg gaaatcgacg
    20221 aatatcgttt cgctgtagcc caattgggat gccagccgct gccggtccgc gacgccgacc
    20281 aggctcgcgt cgaccacgcc gagcggattg ccgaaattcc cgtccgaatc cgtgaacacc
    20341 cgcaccaccg ccacgtcgat acccatggcc cgactgtacg gccccggctg aggccgcccg
    20401 ggtgatggtg gttcaggtgg cgctgcgctc gtcggaaccc atggcgttca gcacggcgac
    20461 gcgggtgtcg gccgggccgg tgacggtctt ctcgccgccg ccggtgcgga gcaactcacg
    20521 catggtggcc tgatcgtatt gggcgggctg ggtggtgtcg atgaagcgct ggacgatgtc
    20581 gatgaagcgg gcggggtcgt cgtggaacgg gaagtggccg gagccttcga agatctcgag
    20641 gagcgagccg ggcatcgcgg catgcgccat ccaggcgtga cggacgggga cgaccacatc
    20701 cttggtgccc cagacgatct gcaccggggt gccctcggcc aaataacatc ggtccagcat
    20761 ggtcacgatc tgcccgcgcc aatccaccac cgcccgcagg gtccggctga acgccgccga
    20821 cgccgtcggt tccggcaggt catccaagat ccgcagcacg ttgggcagat cgcggcccag
    20881 gccggtcgat ccgatcgcca ggcccgccaa ccgtcccgcc agctgaatcg ccggcagcac
    20941 cagaggtagc cgcagcagcg ccagagcctc gccgcccatc ggcaacgagg ccaaccgcag
    21001 gaccaggttg acatccttgg tgacgccacc ggaagcgacc aggatcaacc gctcgaccat
    21061 atgcgggaac tgataggcga actgcatcgc gacgccgccg ccgagcgact gcccgacaat
    21121 ggtgcctcgc tcgaggccga gcgtcatcat gagatcgcgc acgacgctgg caaaggaacc
    21181 gagcgaatag tcgccgtcga ccggcttcgc ggacgaaccg t
//
LOCUS       DASSGQ010000012        10209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_61537, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000012 DASSGQ010000000
VERSION     DASSGQ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61537"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="VFI55_00515"
     CDS             <1..1181
                     /locus_tag="VFI55_00515"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET7072551.1"
                     /translation="PDNTLAQVDALCARFRISGLPVVDDSGALVGIITNRDMRFEVDQ
                     TKQVAEVMTKAPLITAQEGVSADAALGLLRRNKIEKLPVVDGHGRLTGLITVKDFVKT
                     EQHPLATKDSDGRLLVGAAVGVGGDAWVRAMMLVDAGVDVLIVDTAHAHNRLVLDMVG
                     KLKAEVRGRVEVIGGNVATRAAAAALVDAGADAVKVGVGPGSICTTRVVAGVGAPQIT
                     AILEAVAVCAPAGVPVIADGGLQYSGDIAKALAAGASTAMLGSLLAGTAEAPGDLIYV
                     NGKQFKSYRGMGSLGAMQGRGGGKSYSKDRYFADDALSEDKLVPEGIEGRVPFRGPLS
                     SVIHQLTGGLRAAMGYTGSPTVEVLQQAQFVRITPAGLKESHPHDVAMTVEAPNYYAR
                     "
     gene            1196..2323
                     /locus_tag="VFI55_00520"
     CDS             1196..2323
                     /locus_tag="VFI55_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HET7072552.1"
                     /translation="MVEIGMGRAARRTYELSEISIVPSRRTRSSQDVSTAWQLDAYRF
                     EIPVLAHPTDALVSPEFAIELGRLGGLGVLNGEGLIGRHADVEAKIAQLVEAATKEPE
                     PSAAIRLLQELHAAPLDPDLLGAAVARISEAGVTTAVRVSPQNAQALTPVLLQAGIDL
                     LVVQGTIISAERVASDGEPLNLKTFISELDVPVVAGGVLDHRTALHLMRTGAAGVIVG
                     YGSTRGVTTSDEVLGISVPMATAIADAAAARREYLDETGGRYVHVLADGDIHTSGELA
                     KAIACGADAVVLGTPLAESAEALGAGWFWPAAAAHPSLPRGALLQIALGERPPLRQVL
                     NGPSDDPFGSLNLVGGLRRSMAKAGYCDLKEFQKVGLAVGS"
     gene            2455..4209
                     /locus_tag="VFI55_00525"
     CDS             2455..4209
                     /locus_tag="VFI55_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET7072553.1"
                     /translation="MKPDYPDFDVLIIGSGFGGSVSALRLTEKGYRVGVLEAGRRFSD
                     EEFAETSWDLRKFLWAPKLGCYGIQRIHPLRNVMILAGAGVGGGSLNYANTLYVPPEP
                     FFADQQWAHITDWRSELMPHYQQAQRMLGVVQNPTFTDADRIVKEVADEMGCGDTWVP
                     TPVGVFFGPDGTKAPGKTVPDPFFGGAGPDRTGCLECGCCMTGCRYGAKNTLLKNYLG
                     LAESAGAQVIPMTTVKDFHERSDGLWEVHTVRTGSWLRRDRRTLTATYLILAAGTWGT
                     QHLLFNMRDKGKLPRLSERLGMLTRTNSESIVGAATLEVSPDLDLTHGVAITSSIHPT
                     SDTHIEPVRYGKGSNAMRLLQTLMTDGPGPEGPGGPYVPRWKQLLHQAREDPRGIIRM
                     INVHQWSERTVIALVMQHLDNSITTFTKRGKLGIRWYTSKQGHGEPNPSWIPVGNEVT
                     RRIAEKIDGVAGGTWGELFNIPLTAHFLGGAVIGDSPEHGVIDPYHRVYGYPTLSVVD
                     GAAISANLGVNPSLSITAQAERAASLWPNKGENDERPLQGDTYRRVAPIPPAHPVVPL
                     DAPGALRWLPIDPISTAG"
     gene            complement(4227..5129)
                     /locus_tag="VFI55_00530"
     CDS             complement(4227..5129)
                     /locus_tag="VFI55_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET7072554.1"
                     /translation="MTSQITVTKLGSRIGARVDGVRLGGDLEPAAIDRIRQALLTHKV
                     VFFRHQHHLDDEQQLAFAGFMGTPIGHPAAAALAANNSPIITPINSEYGKATRWHTDV
                     TFAANYPAASILRAVTLPSYGGSTLWASTAAAYDQLPDPLRSLVDNLWALHTNRYDYV
                     TTEAAMSMTDTQRAFRQAFEKPDFRTEHPVVRVHPETGERTLLAGDFVRSFVDLDSHE
                     SSVLLELLQRRITMPENTIRWTWAPGDVAIWDNRATQHRAIDDYDNQPRLMHRVTLMG
                     DVPVNVYGERSRVVSGAPLRAIAS"
     gene            5197..5763
                     /locus_tag="VFI55_00535"
     CDS             5197..5763
                     /locus_tag="VFI55_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7072555.1"
                     /translation="MTTQSASGRRNIPTGREEVAAAILEAATDLFAERGPAATSIRDI
                     AARSKVNHGLVFRHFGAKEQLVGAVLDHLGANLAELLESGAPPDVVERALDRQMRVMA
                     RTVLDGYPAAQLQSRFPNIPGLLDAVRPRHDTEIGARLAVANALALQVGWRLFAPILR
                     SATGLDELTDADIREAVEAAVARVVEPH"
     gene            complement(5757..8123)
                     /locus_tag="VFI55_00540"
     CDS             complement(5757..8123)
                     /locus_tag="VFI55_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HET7072556.1"
                     /translation="MITEEEFPVEPWQVRETRLDLNLLAQSESLFTLSNGHVGLRGNL
                     DEGEPYGLPGTYLNSFYEIRPLPYAEAGYGYPEAGQTIVDVTNGKIIRLLVEDEPFDV
                     RYGELISHERVLDLRAGTLTRRADWRSPAGKQVRVVSTRLVSLAQRGVAAIEYVVEAV
                     GAFVRVTVQSELVTNEDQPETSGDPRVSAILDKPLEAVDHEIAERGIVLMHRTRSSAL
                     MMAAAMDHEVEVPGRVEVTTDARADLARTTVICGLRAGQKLRIVKYLAYGWSSMRSRP
                     ALRDQAAAALHSARYSGWQGLLDSQRAYLDDFWENADVEVEGDPESQQAVRFGLFHLL
                     QASARAERRAIPSKGLTGTGYDGHAFWDTEGYVLPVLTYTAPHAVADALRWRASTLPL
                     ARDRAAELGLKGASFPWRTIRGQECSAYWPAGTAAFHINADIAMAFERYRAVTGDHSL
                     EKECGLEVLVETARLWMSLGHHDRHGVWHLDGVTGPDEYTAVVRDNVFTNLMAAHNLV
                     SAAEACNRHPEAAELMGVTNEETSAWRDAAEAVHIPYDAELGVHQQCEGFTTFAEWDF
                     EANQTYPLLLHEAYVRLYPAQVIKQADLVLAMQWQSHAFTPEQKARNVDYYERRTVRD
                     SSLSACTQAVMCAEVGHLELAHDYAYEAALIDLRDLHANTRDGLHMASLAGAWMALVV
                     GFGGLRDDEGILSLDPQLPDGISRLRFRLRWRDFRLTVDVNHSDVTYTVRDGPGAELT
                     IRHAGKELMLTSESPTTVAVRIRKPLLPPPHQPPGREPMHRRALARGQ"
     gene            complement(8137..8880)
                     /locus_tag="VFI55_00545"
     CDS             complement(8137..8880)
                     /locus_tag="VFI55_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HET7072557.1"
                     /translation="MSSGLGLPENVRACLFDLDGVLTDTASVHTKAWKAMFDAYLSER
                     AKRTGEKFVPFDAAADYRQYVDGKKREDGVRSFLNSRGIDLPDGNPDDPDDAETIYGL
                     GNRKNEMFQKVLQDEGVEVFDGSRRYLEAVSAAGLGIAVVSSSANTRDVLHITGLDRF
                     VQQRVDGITLRDEHIAGKPAPDSYLRGAQLLNVGPDAAAVFEDALSGVQAGRAGKFGY
                     VVGVDRVGQADDLRRNGADVVVTDLAELL"
     gene            complement(8973..9821)
                     /locus_tag="VFI55_00550"
     CDS             complement(8973..9821)
                     /locus_tag="VFI55_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7072558.1"
                     /translation="MNDNVEEQYSTGLTRRNIERALIGAGKDLDHLEPSDLGLLEDFH
                     TMGRYATGQLVDLAGITRESAVLDAGSGVGGTARYIASHCGCTVTAVDLTAEYCQTSR
                     WLNRLVGLDDRISVRQADVTELPFADRTFDVAISQHVQMNVADKVRLYSEARRVLALG
                     GRLAIWDITIEKSGTIEKSGTIGDPGELDYPLPWADQPARSHLVTSDELRAAVESAGF
                     AVERWNDLTEQAASLMGGMLAQPPNPLGLHAFVTDFRRKAENLTQALADGRLRAIQAV
                     ARAIES"
     gene            complement(9818..>10209)
                     /locus_tag="VFI55_00555"
     CDS             complement(9818..>10209)
                     /locus_tag="VFI55_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylase"
                     /protein_id="HET7072559.1"
                     /translation="SENDITGTSSVPDMPLFGRVTAGIVRAFEAMGLSPRFGATLHTV
                     FADAGLGAPHLTLGAPIGTAVDTDILAYAAEVWRLVSPVAQQTGPAIEEIADIDDFVP
                     QFRREALAANALITMPPLITAWAEVRT"
BASE COUNT         1605 a         3362 c         3555 g         1687 t
ORIGIN      
        1 ggccggacaa caccctggcc caggtggacg cgctgtgcgc gcggttccgg atctccggtt
       61 tgccggtggt cgatgattcc ggcgcgctgg tcgggatcat caccaaccgc gacatgcgct
      121 tcgaggtgga ccagaccaaa caggtcgccg aggtgatgac caaggccccg ctgatcaccg
      181 cccaggaggg cgtcagcgcc gacgcggcgc tggggttgtt gcgccgcaac aagatcgaga
      241 agctgcccgt cgtcgacggc cacggccggc tcacgggcct gatcaccgtc aaggacttcg
      301 tcaagaccga acagcacccg ttggccacca aggacagcga cggccggctg ctggtcggcg
      361 ccgccgtcgg tgtcggcggt gacgcctggg tccgcgcgat gatgctcgtc gacgccgggg
      421 tcgacgtgct gatcgtggac accgcgcacg cgcacaaccg gctggtgctc gacatggtcg
      481 gcaaactcaa ggctgaagtc cgcggccgcg tcgaggtgat cggcggcaac gtggccacca
      541 gggccgcggc cgcggcccta gtcgacgcgg gagccgacgc ggtgaaggtc ggtgtgggac
      601 cgggttccat ctgcaccacc cgggtggtcg ccggtgtcgg cgcgccgcag atcaccgcga
      661 tcctggaggc cgtcgcggtg tgcgcaccgg ccggggttcc ggtcatcgcc gacgggggac
      721 tgcagtactc cggcgacatc gccaaggcgt tggccgccgg cgcgtcgacg gccatgctgg
      781 gttcgttgct ggccggcacc gccgaggcgc ccggcgatct gatctacgtc aacggcaagc
      841 agttcaagag ctatcgcggc atgggctcgc taggcgctat gcagggccgg ggcgggggca
      901 agtcgtactc caaggaccgc tacttcgctg acgacgcgct ctccgaggac aagctggtgc
      961 ccgagggcat cgagggcagg gtgccgttcc gcggaccgct gtcctcggtg atccaccagc
     1021 tcaccggggg ccttcgggcc gcgatggggt acaccggctc gcccaccgtc gaggtgctgc
     1081 agcaggcgca gttcgtccgc atcacgcccg ccggactcaa agagagtcac ccgcacgacg
     1141 tcgcgatgac cgtcgaagcg cccaactact acgcgcggtg aacagcgaac tggccatggt
     1201 cgagatcggg atgggccgcg ccgcccgtcg cacgtacgaa ctcagcgaaa taagcattgt
     1261 gccgtcgcgc cgcacccgct catcgcagga tgtctcgacg gcgtggcagc tcgacgccta
     1321 ccggttcgag atcccggtgc tggcgcatcc gaccgacgcg ctggtgtccc cggagttcgc
     1381 gatcgagctc ggccggctgg gcgggctggg cgtgctcaac ggcgaggggt tgatcggccg
     1441 gcacgccgac gtcgaagcca agatcgcgca acttgtcgag gccgcgacaa aggagcccga
     1501 accctcggcg gcgatccggt tgctgcagga actgcacgcg gcgccgctgg accctgacct
     1561 gctgggcgcg gcggtcgcgc gcatcagtga ggccggggtc accaccgcgg tgcgggtcag
     1621 cccgcaaaac gcccaggcgc tgacgccggt gctgctgcag gccggcatcg acctgctggt
     1681 ggtccagggg acgatcatct cggctgagcg ggtggccagc gacggtgagc cgctgaacct
     1741 gaagaccttc atctcagagc tcgacgttcc ggtggtcgcc ggcggcgtac tcgaccaccg
     1801 caccgcgctg catctgatgc gcaccggcgc cgccggggtc atcgtcggct acggctccac
     1861 ccgaggggtg accacctccg atgaggtgct gggcatcagt gtgccgatgg ccaccgcgat
     1921 cgccgacgcg gccgccgccc gtcgcgaata cctggacgag accggcggcc gatacgtgca
     1981 cgtgctggcc gacggcgata tccacacctc cggcgagctt gccaaggcca tcgcctgtgg
     2041 tgccgacgcg gtggtgctgg gcacgccgct ggccgagtcc gcggaggcgc tgggcgccgg
     2101 gtggttctgg ccggccgcgg cggcgcaccc gtcgttgccg cgcggcgcgt tgctgcagat
     2161 cgccctgggc gaacgcccgc cgctgcggca ggtgctcaac ggtccgtccg acgatccgtt
     2221 cggcagcctg aacctggtcg gcggcctgcg ccgatcgatg gccaaggccg gctattgcga
     2281 cctcaaggag ttccagaagg tcggcctggc cgtcggaagc tgaagcaagc tgagccaacc
     2341 ccgccgagcg tgcagctggc cgcacactgg tagctgaacg tgcggctggc cgcgcgctcg
     2401 cgacggagcc ggggccccta gttgggtacc cgctggtgag gccatactgg tgcgatgaag
     2461 ccggattacc ccgattttga tgtcttgatc atcggttctg gattcggggg gagcgtcagc
     2521 gcgctgcggc tgacggaaaa gggttaccgc gtcggcgtgc tggaggcggg gcgccggttc
     2581 tccgacgagg agttcgccga gacctcctgg gacctgcgca agtttctgtg ggcaccgaaa
     2641 ctcggttgct acggcattca gcggatccac ccgctgcgca acgtgatgat cctggccggc
     2701 gccggggtgg gcggcggttc gctgaactac gccaacacgt tgtatgtgcc gcccgagccg
     2761 ttcttcgccg accagcagtg ggcgcacatc accgattggc gcagcgagct gatgccgcac
     2821 tatcagcaag cgcaacggat gctgggcgtg gtgcagaacc cgacgttcac cgacgccgac
     2881 cgcatcgtca aagaagtcgc ggacgagatg gggtgcggcg acacctgggt accgacgccg
     2941 gtcggggtgt tcttcggccc ggatggcacc aaggcgccgg ggaaaacggt gcccgatccg
     3001 ttcttcggcg gcgcggggcc cgaccgcacc ggctgcctgg aatgcggctg ctgcatgact
     3061 ggatgccgct acggcgccaa gaacaccctg ctgaagaact acctcggcct cgcggaatcg
     3121 gccggtgcac aagtcatccc gatgacgacg gtgaaggatt tccacgagcg ctccgacggg
     3181 ctgtgggagg tgcacaccgt tcgcaccgga agctggctgc gtcgggacag gcgcacgctc
     3241 accgccacct acctgatcct ggccgcgggc acctggggaa cgcagcatct gctgttcaac
     3301 atgcgcgaca agggcaaact gccgcggctg tcggagcgcc tgggcatgct gacccgtacc
     3361 aactccgagt cgatcgtcgg cgccgcgacg ctcgaggtct cgcccgacct ggacttgacc
     3421 cacggcgtgg cgatcacgtc gtcgattcat ccgacgtcgg acacccacat cgagcccgtc
     3481 cgctacggca agggatccaa cgcgatgcgg ctgctgcaga ccctgatgac cgacggccca
     3541 ggtcctgagg gccccggggg gccctatgtg ccacggtgga agcagttgtt gcaccaggcc
     3601 cgcgaagatc cgcgcggcat cattcggatg atcaacgtcc atcagtggag cgagcgcacg
     3661 gtgatcgcgc tggtgatgca gcacttggac aactcgatca ccacattcac caaacgcggg
     3721 aagctgggca tccgctggta cacgagcaag cagggacacg gcgagcccaa cccgtcatgg
     3781 atcccggtcg gcaacgaggt cacccgccgc atcgccgaga agatcgacgg cgtcgccggc
     3841 ggcacctggg gcgagctgtt caacatcccg ctgaccgcgc atttcctcgg cggagcggtg
     3901 atcggcgaca gccccgagca tggggtcatc gacccctatc accgcgtcta cggctacccg
     3961 accctgtccg tggtggacgg cgcggcgatc tcggcgaatc tcggcgtgaa cccgtcgctg
     4021 tccatcaccg cgcaagccga gcgggccgcc tcactgtggc cgaacaaggg cgagaacgac
     4081 gagcgtccgc tgcagggcga cacctaccgc agggtggctc cgatcccgcc ggcgcacccg
     4141 gtggtacccc tcgacgcacc gggtgcgctg cgctggctgc cgatcgatcc gatcagcacg
     4201 gccggctgac gtgttagctc gtcaggtcag cttgcgatcg cccgcagcgg tgcgccgctg
     4261 accacccggc tgcgctcccc gtacacattg acgggcacgt cgcccatcag cgtgacgcgg
     4321 tgcatgagcc ggggttggtt gtcgtagtcg tcgatcgccc ggtgctgggt tgcccggttg
     4381 tcccagatgg caacgtcgcc cggcgcccag gtccagcgga tggtgttctc gggcatggtg
     4441 attcgccgtt gcagcaattc cagtagcacg ctggattcgt ggctgtccag gtccacgaag
     4501 ctacgcacga aatctcctgc cagcagggtg cgctcgccgg tctccgggtg cacccgcacc
     4561 acgggatgct cggtccggaa atccggcttc tcgaacgcct gccggaacgc ccgctgcgtg
     4621 tctgtcatgg acatggctgc ttcagtggtc acgtagtcgt agcggttggt gtgtagcgcc
     4681 cacaggttgt cgaccagcga tctgagcgga tcgggcagct ggtcgtaggc cgccgcggta
     4741 gaggcccaca gtgtggaccc gccatagctg ggcagcgtga ccgcccgcag gatcgaggcc
     4801 gcggggtagt tggcggcgaa cgtgacgtcg gtgtgccagc gcgtcgcctt gccgtactcg
     4861 gagttgatcg gcgtgatgat cggcgagtta ttggcggcga gggcggcggc cgccgggtgg
     4921 ccgatcggcg tgcccatgaa cccggcgaaa gcgagctgtt gttcgtcgtc gaggtgatgc
     4981 tggtgacgga agaagaccac cttgtgggtc agcagcgctt ggcggatccg gtcgatcgca
     5041 gccggctcca ggtcgcctcc gaggcgcacc ccgtcgactc gggcaccgat acggctgccc
     5101 agtttggtga cggttatctg acttgtcatt gctgtcgtcc ttgagtgaag cggtgtgtag
     5161 tcaaatgact acgtcgttgt cgtgtagtgt agtcatatga ctacacagtc ggcaagtggg
     5221 cgccgaaaca ttcccaccgg gcgcgaagag gtggccgccg cgatcctgga ggcggccacc
     5281 gacctattcg ccgagcgcgg tccggccgcg acatcgatcc gcgatatcgc cgcccgatcg
     5341 aaggtcaacc acggactggt gtttcgtcac ttcggcgcca aggaacagct ggtcggtgcc
     5401 gtgctcgatc acctgggcgc gaatctggcc gagctgctgg agtcgggcgc gccgcccgac
     5461 gtcgtggaac gggctctcga ccggcagatg cgggtgatgg cgcggacggt cctcgacggc
     5521 taccccgccg cgcagctgca gagtcgcttc cccaacatcc cggggttgct cgacgcggtg
     5581 cggccacgac acgataccga gatcggtgcg cgcctggccg tggccaacgc ccttgcgctg
     5641 caagtcggtt ggcggttgtt cgcgccgatc ctgcgctcgg ctacgggtct agacgaactg
     5701 actgacgccg acataaggga ggcggtggaa gccgcggtgg cccgggtcgt cgaacctcac
     5761 tgaccccggg ccagagcgcg gcggtgcatc ggctcgcgcc ccggcggctg gtggggtggc
     5821 ggcaatagtg gcttgcggat gcgcaccgcg acagtggtcg gcgattcgct cgtcagcatg
     5881 agctccttcc cggcgtgccg gatggtcaat tccgcgccgg gcccatcgcg caccgtgtag
     5941 gtgacgtcgg aatggttgac gtcgacggtc aggcggaaat ctcgccaccg caacctgaac
     6001 cggagccgcg aaatgccgtc gggcagctgg ggatccagcg acaggatgcc ctcgtcgtcg
     6061 cgcaggccgc cgaaccccac caccagcgcc atccaggcac cggccagcga ggccatgtgc
     6121 aacccgtcgc gggtgttggc gtgcagatcc cgcaggtcga tcagtgcggc ctcgtaggcg
     6181 tagtcgtggg ccagctccag atgcccgacc tcggcacaca tcaccgcctg agtgcaggcc
     6241 gacagtgacg agtcacgcac cgtgcgccgc tcgtagtagt cgacgttgcg cgccttctgt
     6301 tcgggcgtga acgcgtggct ttgccactgc atcgccagca ccagatcggc ctgcttgatc
     6361 acctgggcgg ggtagagccg cacgtaggcc tcgtgcaaca gcagcgggta ggtctggttg
     6421 gcctcgaagt cccactccgc aaaggtggtg aacccctcgc actgctgatg gacccccagc
     6481 tcggcgtcgt agggaatgtg gacggcctcg gcagcatcgc gccacgcgga ggtctcctcg
     6541 ttggtgacac ccatcagctc cgccgcctcg gggtggcggt tgcaggcctc ggcggcggag
     6601 accagattgt gtgccgccat cagattcgtg aacacgttgt cgcggacgac cgccgtgtac
     6661 tcgtccggac cggtgacccc gtcgaggtgc cagacgccgt gccgatcgtg gtgcccgagc
     6721 gacatccaca gccgggcggt ctcgacgagc acctcgaggc cgcactcctt ctccagtgag
     6781 tggtcgccgg tgacggcgcg gtaccgctcg aaggccatcg cgatgtcggc gttgatgtgg
     6841 aaggccgcgg tgcccgcggg ccagtaggcc gagcactcct gcccgcggat ggtccgccag
     6901 ggaaagctgg cacccttgag cccgagttcg gccgcccggt cccgggccag gggcaacgtc
     6961 gacgcccgcc atcgcagcgc gtcggccacg gcgtgcggcg ccgtgtaggt gagcaccggc
     7021 aacacgtaac cctcggtgtc ccaaaaggcg tggccgtcgt agccggttcc ggtgagcccc
     7081 ttgctgggga tcgcgcgacg ttcggcgcgg gcgctggcct gcaacagatg gaacagcccg
     7141 aaccgcacgg cttgctggga ctcggggtcg ccttcgacct cgacgtccgc gttttcccag
     7201 aagtcgtcga ggtaggcccg ttgcgagtcc agcagcccct gccagccgct gtagcgggcg
     7261 ctgtgtagcg cggccgcggc ctggtcacgc agtgccgggc gggaccgcat gctcgaccag
     7321 ccgtaggcca ggtatttgac gatgcgcagt ttctgtcccg cgcgcagccc gcagatcacg
     7381 gtggtccggg ccaagtcggc acgggcgtcg gtggtgacct cgacgcgccc gggaacttcg
     7441 acctcgtgat ccatcgcggc ggccatcatc agggcgctgc tccgggtccg gtgcatgaga
     7501 actattcccc gctcggcgat ctcgtggtcg acggcctcga gcggtttgtc caaaatggcc
     7561 gacacccgcg ggtcacccga ggtctccggt tggtcttcgt tggtgaccag ctcggattgc
     7621 acggtgacgc gaacgaatgc gccgaccgct tcgacgacgt actcgatggc ggcgacgccg
     7681 cgctgtgcca gggacaccag ccgggtggag acgaccctga cttgcttgcc cgcgggcgag
     7741 cgccagtccg cgcggcgggt cagtgtcccg gcccgcaggt cgagaactcg ctcgtgggag
     7801 atcaattcgc cgtaacggac gtcgaacggc tcgtcctcga ccaacagtcg gatgatcttg
     7861 ccgttggtga cgtcgacgat cgtttggccg gcctccgggt agccgtaacc ggcctcggcg
     7921 tagggcagcg gccggatttc gtagaaggag ttcaggtagg tgcccggcag gccgtacggc
     7981 tcgccctcgt cgaggttgcc gcgcaacccg acgtgcccgt tggacagggt gaacagggat
     8041 tccgattggg ccagcaggtt gaggtcgagc cgggtctcgc ggacctgcca cggttcgacg
     8101 gggaattctt cctcagtaat catcggggat gatgcctcac agcagctcgg cgagatcggt
     8161 gaccacgacg tcggcgccgt tgcggcgcaa atcgtctgcc tggcccactc ggtcgacgcc
     8221 caccacgtag ccgaacttac cggcgcggcc ggcctgcacg cccgacaacg cgtcttcgaa
     8281 gacggccgcg gcgtcggggc cgacattgag cagctgggcc ccgcgcaggt aggagtcggg
     8341 tgccggcttg ccggcgatgt gctcgtcgcg cagggtgatg ccgtccactc gctgctggac
     8401 gaaacggtcg agaccggtga tgtggaggac gtcgcgggta ttggcgctcg acgacaccac
     8461 ggcgataccg agacccgcgg ctgagaccgc ctccagatag cgccgcgacc cgtcgaatac
     8521 ctcgacgccc tcgtcttgca gcaccttctg gaacatctcg ttcttgcggt tgcccaggcc
     8581 gtatatcgtc tcggcgtcgt cagggtcgtc cgggttgccg tcgggcagat cgatgccgcg
     8641 gctgttcaga aacgaccgga ccccgtcctc gcgtttcttg ccgtccacat attggcggta
     8701 gtccgccgcc gcgtcgaacg gcacgaactt ttcgcccgtt cgcttggccc gttcggacag
     8761 ataggcgtcg aacatggcct tccaggcctt ggtatgaacg ctcgcggtat cggtgagcac
     8821 cccgtcgagg tcgaacagac aggcacgcac gttctctggc agacccagcc ctgaactcac
     8881 agggggacct cactctcggg tatcggggaa cgttgtccac tccaccatgc cgagcgggtg
     8941 tgccgccagg gttcacccgt ggtcgggacg catcatgact cgatcgcccg cgcgaccgcc
     9001 tggatggcgc gaagacgtcc gtccgccaga gcctgggtga ggttttcggc tttccgcctg
     9061 aagtccgtga cgaaggcgtg caggcctaag gggttggggg gctgcgccaa catgcctccc
     9121 atgagcgagg ccgcctgctc ggtcaggtcg ttccaccgct cgaccgcgaa cccggcggac
     9181 tcgacggcgg cacgcaattc gtctgaggtg accagatggc tgcgtgcggg ttggtcggcc
     9241 cacggcagtg gatagtcgag ttcacctggg tcgccaatcg tcccgctttt ttcaatcgtc
     9301 ccgctttttt caatcgtgat gtcccagatg gcaagccgac cccctagcgc cagcacccgg
     9361 cgcgcttcgg agtacaggcg caccttatcg gcgacgttca tctggacatg ctggctgatg
     9421 gcgacgtcga aagtgcggtc ggcgaaggga agttcggtga cgtcggcctg acggaccgat
     9481 atccggtcgt cgagcccgac gagccggttg agccaacggc tcgtttgaca gtattcggcg
     9541 gtgagatcca cggcggtgac cgtgcagccg cagtgggagg cgatgtagcg ggcggtgccg
     9601 ccgacgccgc tgcccgcgtc gagcaccgca ctctctcttg tgattccggc caggtcgacc
     9661 aattggccgg tggcgtagcg ccccatggtg tggaagtcct ccagcagacc caggtcggat
     9721 ggctcgaggt ggtcgaggtc tttcccggcg ccgatcagcg cccgctcgat gttgcgtcgc
     9781 gtcagccccg tcgagtactg ctcctccacg ttgtcgttca tgtgcgcacc tcggcccatg
     9841 cggtgatgag tggtggcatc gtgatcaggg cgttggccgc gagcgcctcg cggcggaatt
     9901 gcgggacgaa gtcgtcgatg tcggcgattt cctcgatggc ggggcccgtc tgttgtgcga
     9961 cgggggatac caaccgccag acctccgcgg cgtaggccag gatgtcggtg tcgacggcag
    10021 tgccgatcgg cgcgccgagg gtcaagtgcg gagcgcccag accggcatcg gcaaagaccg
    10081 tgtgcagcgt ggcaccgaac cgcggactga gccccatggc ctcgaaggcg cggacgatcc
    10141 ccgccgtcac ccgcccgaac agcggcatgt ccgggacact gctggtcccg gtgatgtcgt
    10201 tctcggaga
//
LOCUS       DASSGQ010000013        27966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1337761,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000013 DASSGQ010000000
VERSION     DASSGQ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 27966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27966
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337761"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..304
                     /locus_tag="VFI55_00560"
     CDS             32..304
                     /locus_tag="VFI55_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072560.1"
                     /translation="MAVAVFLAGSGHEEIDGAEAVVTDASGGLGGAIAGARYARGALA
                     ILAVDGENLSRLWRRSLGGAEIVVCDLADRGQRDYDNGRALIAEQL"
     gene            complement(910..2229)
                     /locus_tag="VFI55_00565"
     CDS             complement(910..2229)
                     /locus_tag="VFI55_00565"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HET7072561.1"
                     /translation="MTPTAGERFYRHGLHRLSGRDVNEQHRVSTTLELFFDLAFAIAF
                     GAAGAQFAQGIAAGHAGIATLAFIVAMIATVWAWTGYSWFASAFDNDDWLFRVLTLVQ
                     MAGVIVLAIGIPQLFTVVENGTALTGRVMVAGYVIMRVAVVAQWTRVMLTDGTYRRLA
                     LGNTLSVGGAQLGWVTLTFVNPGTGIGAIVAYGVLWVIDLGGPMVAASVAARRGAALP
                     WHPHHLAERYSLLAIIAIGETIAGTLAAAQMISGAQGWSVTTVLVTGIGIAMSFCLWW
                     TYFLIPSAPILSVRRSKVIPWAVGHSVLFASIAAAGAGFRVIGYYYDHQYSVSTVIAV
                     AAVALPVFTFLATVYASYVWLVNDVSFDIGDVVGFVAPIAAIGLASIGGPLWACLFLA
                     LASPVTLIVSYELGKWKSLAAQLDTALYKATTDRDPADEPPTRKPAG"
     gene            complement(2758..5703)
                     /locus_tag="VFI55_00570"
     CDS             complement(2758..5703)
                     /locus_tag="VFI55_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND family transporter"
                     /protein_id="HET7072562.1"
                     /translation="MTSDTTDTSDTDEIPVAPRTEVERKPRVARTIRVLALPIVVIWL
                     LIAVGVNVFVPQLEKVAEDVSVPLSPSDAPSVTGMKHIGEKFKEYDSDNLVLVTLVGD
                     RPLGQDAHQYYDDLVKKLKQDTQHVEHTLDFWGQRFTASGVESYDHKSAYIQVNLRGD
                     QGGAVGDKSVDSVRKIVADSKPPPGVKAYVAGQGALTDDTIVVGNASLFKMTIITIVI
                     IAIMLMFVYRSIGTVLLTMVILFVGLATGRGVVALLGDTGIMGLSTFAVNLLTALAIA
                     AGTDYAIFMVGRYQEGRERGLDREAAYYDTFTGVTKVVLGSGLTIVGALACLHFTRLP
                     YFSSLAFPCAIGLLVVVAAGVTLTPALIVFASGFGLFDPKRKFNYTRWRRLATAIVRW
                     PAPILATSVVLAIVGALGLMGFNPRYNDLYYLPKSASSIQAYTAADQHFTQARLNPDL
                     LMIESDHNLRNPTDMLVLDKIASALFHVPGIERVQSITRPLGPPIQDGSIPFQLSVQS
                     APIRDNLSYLKGRIADIKKITGYLDTLISLLERQYKVTQDLANAADDSSKTAANTAAI
                     TDEIRDHIADFDDFWRPIRAYFYWEKHCFDIPICWSLRGLFDALDGFDKLAEQFHHLT
                     NDLGDTAKATHELLAIIPQNIATSKAIRDTTLTIYSSFDSLIDQFDKLTDTSAVMGKS
                     FNDSQVESLFYLPPEIFQNPDFQLGLSLMVSPDGKAARFIITHAVDPATTEGISSVAQ
                     ERQATKEALKLTSLQNADIYLGGTAATFYDIANGAKYDLMIAGVASIVLIFIIMVIVT
                     RALVAASVIVGTIVMSLGAAFGLSTLIWQHLLNFQLHWVATEFALIVLLAVGSDYNLM
                     LVSRIEEEIGAGLKTGLIRGMGVTGPVVTAAGLVFAVTMATMITSDLRAIGQFGTTIG
                     IGLLFDTFVVRSLITPSIMTVMGRWFWWPKRVRVRPASQMLRSVGPRPLVRALLYQPR
                     HADPLDPQT"
     gene            complement(5700..6125)
                     /locus_tag="VFI55_00575"
     CDS             complement(5700..6125)
                     /locus_tag="VFI55_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmpS family transport accessory protein"
                     /protein_id="HET7072563.1"
                     /translation="MFKLLKRAWVPLVVVVALIIGGVAVDRLNGVFPGPSVPKPDPRD
                     ATPPYAAKTAIYEILGPPGTTGVVNWMDADAQPQKANFTTLPWSQTIVAEMPGIFAYV
                     VAQGDGPSIGCRITVDGKLVDQQQVNTRDAQVSCLDKSA"
     gene            6701..6934
                     /locus_tag="VFI55_00580"
     CDS             6701..6934
                     /locus_tag="VFI55_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric uptake regulation protein"
                     /protein_id="HET7072564.1"
                     /translation="MATKTKVKIKTCVHCGHTFDPNDRRRDLSDPEWLPDASVYCSHE
                     CSDRYHCEMGHCCSTHVKEKARREAAKAAQPKT"
     gene            complement(7269..7345)
                     /locus_tag="VFI55_00585"
     tRNA            complement(7269..7345)
                     /locus_tag="VFI55_00585"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7309..7311),aa:Met,seq:cat)
     gene            complement(7524..8162)
                     /locus_tag="VFI55_00590"
     CDS             complement(7524..8162)
                     /locus_tag="VFI55_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HET7072565.1"
                     /translation="MRLVVAMTGATGAALGIRLLEALRELEVETHLILSDWARATIKL
                     ETDHTVEDVRSLATHAYGAHDLAAGISSGSFRTDGMVVCPCSMKTLSAIRVGYSDNLI
                     TRAADVTLKERRRLVLVPREAPLSEIHLENMHYLARAGAVIFPPTIAYYARPSSVDEV
                     TTHVVGRVIDQLGIEHDLIKRWKDDPDPIAFNGVGPDAAGFHEEPDAVERTN"
     gene            complement(8363..8866)
                     /locus_tag="VFI55_00595"
     CDS             complement(8363..8866)
                     /locus_tag="VFI55_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HET7072566.1"
                     /translation="MMTVSLSANRRDAADIVSFHAPANLFENLQKVLVDLIELHLQGK
                     QVHWNIVGTNFRDLHLQLDSIVDIAREASDTIAERMRALDAVPDGRSDTVVATTTVPA
                     IPPGLVGAAVAVDMITERTYAVVDTMRTVHDDVDAADASTADLLHVIIGDLEKAAWLL
                     KSEIGTA"
     gene            complement(8911..9561)
                     /locus_tag="VFI55_00600"
     CDS             complement(8911..9561)
                     /locus_tag="VFI55_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET7072567.1"
                     /translation="MTAQSIDTIAGIAIPDTALVREATDLIRDAENDLLFDHSRRVFL
                     FGALQGRRLGLEPNLELLYVGAMFHDLGLTERYRTSPRRFEVDSANAARDFLLQHGVD
                     KADAEKVWLGIALHTTPGVPEFLAPEIALVTAGVETDVLGIGREDLSPDALAAVTAAH
                     PRPNFKQRILGAFTDGMKHRPRSTFGTVNADVLQHYDPTFVRDDFVEIILNNTWPE"
     gene            9698..10672
                     /locus_tag="VFI55_00605"
     CDS             9698..10672
                     /locus_tag="VFI55_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HET7072568.1"
                     /translation="MATAEKTERVVVIVVFDGAKLLDAAGPAEVFAEANRYGANYAIQ
                     IASVDGRDVTTSIGTRFAVTCAISSIDSADTVVVTGGDDLIGRPIDPALVEALKTVPG
                     RTRRLASVCTGSFILAQAGLLAGRRATTHWKHTSLLARAFPDVGVEPDAIYVRDGDVF
                     TSAGVTSGIDLALALVESDYGPALVRDVARSLVVYLKRAGGQSQFSVLVEAAPPPGSA
                     LRTVTDAITADPGADHSVRKLADRASLSTRQLTRLFQTELGTTPARYVEMVRIDFARA
                     ALDAGRGVNETAGLAGFGSAETLRRVFVNHIGISPKAYRDRFRTASRN"
     gene            complement(10684..13665)
                     /locus_tag="VFI55_00610"
     CDS             complement(10684..13665)
                     /locus_tag="VFI55_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HET7072569.1"
                     /translation="MGMRPTARMPKLTRRSRTLILIALGVIVLLLAGPRLIDAYVDWL
                     WFGELGYRSVFTTVLVTRLVVFLVAGVLVGGVVFAALALAYRTRPVFVPSSTNDPVAR
                     YRAVVMSRLRLFGIGIPVAIGLLAGIVGQSYWVRIQLFLHGGEFGVKDPQFGKDLGFY
                     AFDLPLYRLVLTYMFVAAFLALVANLLAQYVFGGIRLSGRTGALSRSARIQLVSLVGI
                     LVLLKALAYWLDRYELLSHTRGGKPFTGAGYTDINAVLPAKLILMAIALICAAAVFSA
                     ITLRDLRIPAIGLVLLLLSSLIVGAGWPLIVEQISVRPNAAQKESEYISRSITATRQA
                     YGLTSDVVTYRNYTGDGQATAQQVASDRATTSNIRLLDPTIISPTFTQFEQGKNFYYF
                     PDQLSIDRYLDRNGALRDYVVAARELNPDRLIDNQRDWINRHTVYTHGNGFIASPANT
                     VRGIANDPTQNGGYPEFLVNVVGANGSVVSDGPAPLDQPRIYFGPVMSNTSADYAIVG
                     RNGNDREYDYETNTETKNYTYTGQGGVPVGSWISRSVFAAKFAERNFLFSNVIGSNSK
                     ILFNRDPAQRVEAVAPWLTTDSAVYPAIVNKRLVWIIDGYTTLDNYPYSELTSLESAT
                     ADSTEVAFNRLAPDKQVSYIRNSVKATVDAYDGTVTLYQQDEQDPVLKAWMQVFPGTV
                     KPKSDITPELAEHLRYPEDLFKVQRMLLAKYHVNDPVTFFSTSDFWDVPLDPNPTASS
                     YQPPYYIVAKNIAKQDNSASYQLISAMNRFKRDYLAAYISASSDPATYGKITVLTIPG
                     QVNGPKLANNAITTDPAVSQDLGVIGRDNQNRIRWGNLLTLPVGQGGLLYVEPVYASP
                     GASDAASSYPRLIRVAMMYNDKIGYGPTVGDALTGLFGPGAGAAATGIQPTEAAPPAN
                     APPGAPAPAAAPGSPPEPVAAPPAPDGAVTLSPAKAAALQEIQAAIGAARDAQKKGDF
                     AAYGSALQRLDDAITKYNNTK"
     gene            complement(13943..14965)
                     /locus_tag="VFI55_00615"
     CDS             complement(13943..14965)
                     /locus_tag="VFI55_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET7072570.1"
                     /translation="MNRRILTLIVALVPILAFGVLLAVVTVPFVSLGPGPTFDTLGEV
                     DGKQVVAIEGTQTHPTTGHLNMTTVSQRDDLTLGQALTLWLSGQEQLVPRDLVYPPGK
                     SREDVDKANNADFKNSEDSAEYAALGYLKYAPAVTVATVSDPGPSAGKLKEGDALDAV
                     DGTPVSTVEQFTGLLKNTKPGQVVTIDYRRKNEPAGVAQITLGTNKDRDYGFMGVAVL
                     DAPWAPFVVDFNLANVGGPSAGLMFSLAVVDKLTTGDLAGSTFIAGTGTITIDGKVGQ
                     IGGITHKMVAAHDAGATVFLVPAKNCYEASSDNPSGLRLVKVETLSEAVDALHAITSG
                     GQPPSC"
     gene            15044..16447
                     /locus_tag="VFI55_00620"
     CDS             15044..16447
                     /locus_tag="VFI55_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HET7072571.1"
                     /translation="MSTVDVMADLPFGFSGGNDPERDKHGKKDPDPGSGPSDPLGAFG
                     IGGDFGMGDLGQIFTQLGQMFSNAGTVTAGGKSTGPVNYDLARQVASNSIGFVAPIPA
                     ATNQAIADAVHLAETWLDGATALPAGTTKAVGWSPTDWVDNTLETWKRLCDPMAEQIA
                     TVWASSLPEEATSMAGPLLAMMSQMGGMAFGSQLGQALGRLSREVLTSTDIGLPLGPK
                     GVAAIMPGAVEAFAAGLEQPRSEILTFLAAREAAHHRLFGHVPWLASQLLGAVEAYAM
                     GMKIDMSGIEELARDFNPASLADPAAIENLLGQGVFEPKATPAQQQALERLETLLALV
                     EGWVQVVVTAALGDRIPGTTALSETLRRRRASGGPAEQTFATLVGLELRPRKLREAAA
                     LWERLTQAAGADARDAIWQHPDLLPSTDDLDEPAGFIDRVIGGDTSGIDEAIAKLEEC
                     ADTQDRDSGPDGGPVDN"
     gene            16496..17338
                     /locus_tag="VFI55_00625"
     CDS             16496..17338
                     /locus_tag="VFI55_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodehydratase"
                     /protein_id="HET7072572.1"
                     /translation="MASSYALDPALPVLLRPDGAVQVGWDPRRAVLVRPPGGLPAAEL
                     AALLRSMRSPTPIAELQRRAGDRLQDADGLTNLVAQLVGAGVATECGRSRGRAASIRI
                     HGRGPLSDLLVEALRCSGARIAHSSQPHAAVSPTEVDLVVLSDYLVADPRMVRDLHSR
                     GVAHLPVRVRDGTGLVGPLVIPGVTSCLGCADLHRSDRDAAWPAIAAQLRDTVGVADR
                     ATLLATAALALSQVNRVIAAVRGQAGVPDPAPPPALNATLEFDLNAGSIVARQWTKHP
                     LCSC"
     gene            complement(17356..17529)
                     /locus_tag="VFI55_00630"
     CDS             complement(17356..17529)
                     /locus_tag="VFI55_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072573.1"
                     /translation="MAAKQTQHDAADALFRAIIETLDKNRTAGTLTAGVLEELSRAYA
                     SVATNVPDQGRMG"
     gene            17694..19037
                     /locus_tag="VFI55_00635"
     CDS             17694..19037
                     /locus_tag="VFI55_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HET7072574.1"
                     /translation="MDDGCVADIKRGRAARNAKLASIPVGFAGRAALGFGKRLTGKSR
                     DEVQAELLEKAANQLFTVLGELKGGAMKVGQALSVMEAAIPEEYGEPYREALTKLQKD
                     APPLPAHKVHRVLDAQLGTKWRDRFGSFDDTPVASASIGQVHKAVWSDGREVAVKIQY
                     PGADEALRADLKTMQRMVSVLRQLSPGADVQGVVDELIQRTEMELDYRLEAENQRAFA
                     KAYHGHPHFAVPRVVASAPKVVIQEWIQGVPMAEIIRDGTRADRDLIGTRLLELTFDA
                     PRRLGMMHGDAHPGNFMLLPDGRMGVIDFGAVAPLPGGYPIELGMIMRLAREKNYDLL
                     LPTMEKAGFIQRGRQVSVRDIDEMLRQYVEPIEVDVFHYTRKWLQRMTVSQIDRSVSQ
                     IRTARQLDLPVKLAIPMRVISSVAAILCQLDAHVPIRALSEELIPGFAEPDTAVV"
     gene            complement(19058..19336)
                     /locus_tag="VFI55_00640"
     CDS             complement(19058..19336)
                     /locus_tag="VFI55_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HET7072575.1"
                     /translation="MSAPPVPKPTLPELPCHADDPDLWFAEAPADLERAKTLCADCPI
                     RRQCLTAALERAEPWGVWGGEILERGSIVSRKRPRGRPRKGQQSPVAA"
     gene            complement(19485..19631)
                     /locus_tag="VFI55_00645"
     CDS             complement(19485..19631)
                     /locus_tag="VFI55_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072576.1"
                     /translation="MITNTFGAGVAAAVLTAATFHAARTAAPAAAVKHRAAATDASVD
                     RGHT"
     gene            complement(19758..21872)
                     /locus_tag="VFI55_00650"
     CDS             complement(19758..21872)
                     /locus_tag="VFI55_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HET7072577.1"
                     /translation="MPLVADALTAGLDDEQREAVLAPRGPVCVLAGAGTGKTRTITHR
                     IAQLIASGHVAAGQVLAVTFTQRAAGEMRSRLRRLAATAHTGSGIGTVQALTFHAAAH
                     RQLRYFWPRVVGDTGWQLLDSKFAVVARAASRARLNVSTDDVRDLAGEIEWAKASLIG
                     PEQYPTAVAAAGRDIPMDAAQIATVYTAYEALKVRDESVTLLDFDDLLLHTAAAIEND
                     AAVAEEFRDRYRCFVVDEYQDVTPLQQRVLAAWLGDRDDLTVVGDANQTIYSFTGASP
                     RFLLDFSRQFPDATVVRLERDYRSTPQVVSLANQVIAAARGRVAGSKLQLSGQRAPGP
                     APSFHEHPDETAEAAAVAASIGRLIESGTAASEIAVLYRVNAQSEVYEEALTEAGITY
                     QIRGGEGFFNRQEIKQALLALQRASERASEQPSTGPLPDVVRAVLEPLGLTAEQPAGT
                     RALDRWEALSSLAELVDEEVAQRPELDFAGLLAELRTRADARHPPVVQGVTLASLHAA
                     KGLEWDAVFLVGVADGTLPISHALAHGADSEAVEEERRLLYVGITRARVHLALSWALA
                     RSPGGRQSRKPSRFLNGIAPQTRPDPAPNRPRRNRGTASRCRVCNNNLTTPAAIMLRR
                     CETCAADIDEELLLRLKAWRMDVAKEQKVPAYVVFTDNTLIAIAELLPDDETALIAIP
                     GIGARKLEQYGPDVLELVRQAR"
     gene            22000..22254
                     /gene="mrx1"
                     /locus_tag="VFI55_00655"
     CDS             22000..22254
                     /gene="mrx1"
                     /locus_tag="VFI55_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycoredoxin Mrx1"
                     /protein_id="HET7072578.1"
                     /translation="MTNAAITVYTTSWCGFCHRLRTALKSDAIPYDEIDIDHDSAAAE
                     FVSSVNGGNRTVPTVKFADGSTLTNPSAAEVKAKLAEVAG"
     gene            complement(22251..23174)
                     /gene="nudC"
                     /locus_tag="VFI55_00660"
     CDS             complement(22251..23174)
                     /gene="nudC"
                     /locus_tag="VFI55_00660"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HET7072579.1"
                     /translation="MDFELRDIPVLSRVGADRADQLRTDVDAAAAGWAEAALLRVDSR
                     NQVLVADGRAVLGAAAELAHKPPPDAVFLGRIEGGRHVWAVRGALQAPEDPDVQADVV
                     NLRSLGPIFDDTSSQLVSSAIALLNWHDNARFSAVDGAPTRPARAGWSRINPITGHEE
                     FPRIDPAVICLVHDGGDRAVLARQTVWPERMFSLLAGFVEAGESFEVCVAREIREEIG
                     LHVRDVRYLGSQPWPFPRSLMVGFHAQADPAEDFSFNDGEIAEANWFTRAEVRAALEA
                     GDWSSSSGSKLLLPGSISIARVIIESWAALD"
     gene            complement(23178..24248)
                     /locus_tag="VFI55_00665"
     CDS             complement(23178..24248)
                     /locus_tag="VFI55_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HET7072580.1"
                     /translation="MGNGKSRKLSGLDETLTAQPDHLLVGVLRIPDDHGSPVRVITRR
                     VAIALVVLFGAAVIVYLDRTGYRDVRGGPPTFLDCVYFAAVSLSTTGYGDITPYTETA
                     RLVHTVIFTPLRIAFLAVLVGTTLEVLSERSRQAWKIQRWRSKVRNHTIVIGYGTKGK
                     TAIAAILGDEAAQGEVVVVDTDRNVLEHATTAGLVTVHGDATRSDVLRLAGAQHAASI
                     VVATSRDDTAVLVTLTAREIAPKAKIVASIREAENQHLLQQSGANSVVVSSETAGRLL
                     GLATTTPSVVQMIEDLLTPDAGLAIAEREVEQTEIGGSPRHLRDIVLGVVRDGHLLRI
                     GAPEVDAIEASDRLLYIRHAGH"
     gene            complement(24343..27639)
                     /locus_tag="VFI55_00670"
     CDS             complement(24343..27639)
                     /locus_tag="VFI55_00670"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HET7072581.1"
                     /translation="MNPRYSPAELAGALGLFPPTAEQAAVIAAPPGPLVVIAGAGAGK
                     TETMAARVVWLVANGYAEPGQVLGLTFTRKAAGQLLRRVRSRLARLAGIDLDLTTPAT
                     AAALEPVGTPLVSTYHAFAGSLLRDYGLLLPVEPDTRLLSETELWQLAFDVVNGYGGE
                     LRTDKTPAAITSMVLRLWGQLAEHLVDTRQLRDTHVELERLIHTLPAGPFQRDRGPSQ
                     WLLRLLATQTERTELVPLLDALRERMRAAKVMDFGAQMASAARLAVTFPQVGQELRNR
                     YRVVLLDEYQDTGHAQRIALSALFGGGVDDGLALTAVGDPIQSIYGWRGASATNLPRF
                     STDFPHSDGTPAPVLELRTSWRNPPRALHVANAISAEARRRSVAVHALRPRPDAPPGT
                     VRCALLADVRAEREWIADHLYGQYQRAQADGVSPPTAAVLVRRNADAAPIADALRARG
                     IPVEVVGLAGLLSIPEVADVVAMLRLVADPTAGAAAMRVLTGPRWRLGGRDIVALWRR
                     ALALGEGSGHASSPEALAMAAGPSNIGADAACLADAISDPGPAGSYSAVGYRRIGSLA
                     GELSALRGHLGHSLPDLVAEVRRVLGVDCEVRAAATVPAPGAAGLGAWSGAEHLDAFA
                     DVVAGYGERTSATDTSVAGLLAYLDVAEAVENGLSPAPSTVARDRVPVLTVHSAKGLE
                     WQVVAVAHLSGGIFPSTASRSSWLTDAAELPPLLRGDRASAGSLGIPVLDTADVTNRK
                     QLSDKIAEHRRQLEQRRVDEERRLLYVAITRAEDTLLVSGHHWGATGVKPRGPSDFLA
                     ELKDIVDRAAAAGAPCGMVDEWAPAPSDGERNPLHDNVIEAVWPADPLAARRGDVERG
                     AALVARAMSADTAAPGADVDGWTADVDALLAERARSPRPAARTLPSQLSVSALVDLAR
                     DPAGAAQRLMRRLPTRPDPHAMLGNAFHAWVQQFYGAEWLFDIADLPGAADSDVGDTR
                     ELAALQAAFIESRWAARTPVAVEVPFEMPIGDTVVRGRIDAVFAEPDGGATVVDWKTG
                     EPPHGAEAMRRAAVQLAVYRLAWAALSGVPESSVRAAFYYVRTGATVAPDVLPDAAEL
                     AELQADPAEV"
     gene            complement(27636..>27966)
                     /locus_tag="VFI55_00675"
     CDS             complement(27636..>27966)
                     /locus_tag="VFI55_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET7072582.1"
                     /translation="KDDAQQHAQLAMYQLAIADGLINAGPGDGAEPGGARLVYLGKTG
                     AAGATVREQDPLTTAARDEWRNLVRLAAGTTAGPQFIARRNDGCTHCPIRPCCPAHTG
                     SGGTVAP"
BASE COUNT         4684 a         9101 c         9402 g         4779 t
ORIGIN      
        1 atcggttcgg tgacgaaaag cattgggcgc catggcggtg gcagtgtttc tcgcgggaag
       61 cggtcatgaa gagatcgacg gagcagaagc ggtggtgaca gacgcttccg gtggcctcgg
      121 cggtgcgatt gcaggggccc gatacgcacg cggcgccctc gccatccttg cggtagacgg
      181 ggagaatctc tcaagacttt ggcgtcggag cctgggcggt gccgaaattg tcgtctgcga
      241 tctggcggac cgaggacagc gggactatga caacgggcgc gccctcatcg cggagcagct
      301 gtagataatt tgtccacgac agggacctgc gcgctggatt agcagtcgtc gtgtacgtcg
      361 cggatacctt cgcggcacct acagatatcc tccggacgag acgttggcgg ctgcgatccc
      421 tgcttgcctt cgcgcagcac cacccgggtc ggcggagtgt aggaatcccc tcatcctcgg
      481 gagacttcga ctcctaccca ggcaccgagg cgccgacgag ctctacaccc tcatctgcga
      541 agagccccca aagggcatga aaatggcccc cggagaagtc cgcagacgtc tccgagggcc
      601 atttccgctg gtagcgggga caggattcga acctccgacc tctgggttat gagctaaccg
      661 gtcgcggtct ccgccagtac gctcggtctc gtggctgcat gccagcgacg gtcagccgtc
      721 cgacctgtct cactgcgtcc tgttggttca catcgcttcc acggtgttcc gtgtccaaat
      781 ccgtggccaa cccattgaga cgctctttga atgttgggcc ggtagcgctc tttgagaatc
      841 ggccacgtgg ttggtgtttc gtctgccgtg ttgtttggtc gtagggacgc gattgacgct
      901 tcttctggtt catccagcgg gttttcgcgt aggcggctcg tctgcgggat cgcgatcggt
      961 ggtcgctttg tacagcgcgg tatcgagttg agcggcgagc gatttccatt tgccgagttc
     1021 gtaactgacg atcagggtga ccggggaggc gagcgcgagg aataggcatg cccacagcgg
     1081 cccgccgatc gatgcgagcc ctatcgccgc gatcggcgca acgaacccga cgacgtctcc
     1141 gatatcgaag gagacgtcgt tgaccagcca gacgtaactg gcgtacacgg tagccaagaa
     1201 cgtaaacacc ggcagcgcga cggccgctac cgcgattacc gtgctgacgg aatattggtg
     1261 gtcgtagtag tagccgatga cgcggaaccc ggcgcccgcc gcggcgatcg acgcaaaaag
     1321 taccgagtgc ccgaccgccc aggggatgac tttgctgcgc cgcacggaca ggatcggtgc
     1381 tgagggaatc aggaaatacg tccaccatag gcagaacgac atcgcgatcc cgatcccggt
     1441 gacgagcacg gtggtcaccg accagccttg agcgccggag atcatctgcg cggcggcgag
     1501 cgtacccgcg atggtttcgc cgatcgcgat gatcgccagc aggctgtacc gctcggcgag
     1561 atggtgtgga tgccacggca gcgcggcacc acggcgagcc gccacgctgg cggccaccat
     1621 cgggccaccc aagtcgatca cccacaacac gccataggcg acaatggcac cgattccggt
     1681 tcccgggttg acgaaagtca aggtgaccca acccagttgg gcaccgccga cactgagtgt
     1741 gttgcccaga gccaggcgcc gataagtgcc gtcggtaagc atcacccggg tccactgtgc
     1801 aacgacggcg acgcgcatga tgacgtaccc ggcaaccatc acccggcccg tgagcgcggt
     1861 gccgttctcg acgacggtga acagttgcgg gatgccgatc gccagcacga tcacaccagc
     1921 catctggacg agcgtcagta cccggaagag ccagtcgtcg ttgtcgaacg cagacgcgaa
     1981 ccaggagtag ccggtccacg cccagacggt cgctatcatc gcgacgatga acgcgagcgt
     2041 tgcgatcccg gcgtgtccgg cggcgatacc ctgcgcgaat tgggcaccag cggcgccgaa
     2101 cgcgatggcg aaggccaggt cgaagaacaa ctcgagcgtc gtggagacgc ggtgctgctc
     2161 gttcacgtcg cggccactca agcggtgcaa gccatgccga tagaagcgct ccccagcagt
     2221 aggtgtcacg gtgatgaatt cctccccgaa aaccgcatgg aggcaaccgt acgcttgcac
     2281 caccaccgac tgattggctc cgacacctac gacgcgcacg cagatctgcg catgccgctg
     2341 tgggaacacg gcatgattcg gttggtaggc atgggttttg gtgcctcagt cggtggcacc
     2401 ggggtggtgg ggcgatccaa ggcgcccacg ggccggccgt cgataccgct ctagccggtg
     2461 acgacgcagt cgctgtagag cactgcccaa ccgtgcagcg ctacgtgacc gatttcagcg
     2521 tcgactagcc gcctctagcc gatgtcgtct ctagctcctc gtcaacggcc caccgggcga
     2581 cgcggatcgc cgctcgcgat ccgtcaacag caacgacctg agcattcacg ctaagttcca
     2641 caccatctcg ttcattgcgc cgcgtttcgt tcaaaaattc gttcacaagc tagcggacat
     2701 cgacgtgctg tcggtgcgac acaatgcgca atcggcgggc acgtgttccg ccggatgcta
     2761 cgtctgcgga tccagcgggt ccgcgtgccg cggctggtac aacagcgcac gaaccagcgg
     2821 gcgcggcccg acggaccgaa gcatctggct ggctgggcgg acccgtaccc gcttcggcca
     2881 ccagaaccag cgtcccatca ccgtcatgat cgacggcgtg atgagcgatc ggacgacgaa
     2941 ggtgtcgaac aggaggccga tgccgatcgt cgtgccgaat tggccgatcg cccggagatc
     3001 gctggtgatc atcgtggcca tggtgacagc gaacacgaga ccggccgcgg tcaccaccgg
     3061 gccggtgaca cccatgccgc ggatgagtcc cgttttcagg ccggctccga tctcctcttc
     3121 gattcgggac accagcatca ggttgtagtc cgaccccacc gccaagagta cgattaacgc
     3181 gaattctgtt gccacccagt gcaactggaa attgaggagg tgctgccaga tcagtgtcga
     3241 caacccgaag gcggccccca gcgacatcac gatcgtgccg acgatgacgc tcgcagcgac
     3301 cagagcgcga gtcacgatca ccataatgat gaaaatgagc acgatcgagg ccaccccggc
     3361 gatcatcagg tcgtacttgg cgccgttggc gatgtcgtaa aacgttgcgg ccgtgccgcc
     3421 gaggtagatg tcggcgtttt gcagcgaggt gagtttcagc gcctccttgg tcgcttggcg
     3481 ctcctgagcg acggacgaga ttccctccgt cgtcgccgga tccactgcgt gggtgatgat
     3541 gaagcgagcg gccttgccgt ccggcgacac catcaagctc aaccccagtt gaaagtccgg
     3601 gttctggaag atctcgggcg gcaggtagaa caggctctcc acctgagagt cgttgaagga
     3661 cttgcccatc acggcgctcg tgtcggtgag cttgtcgaac tgatcgatca ggctgtcgaa
     3721 cgagctgtag atggtcagcg tcgtgtcccg gatcgccttc gaggtggcga tattctgggg
     3781 aatgatcgcc agcagttcgt gcgtggcttt agccgtgtcg ccgaggtcgt tggtgagatg
     3841 gtggaactgc tcggcgagct tatcgaaccc gtccagggcg tcgaacagtc ctcgtagcga
     3901 ccagcagatc gggatgtcga agcagtgctt ctcccaatag aagtaagcgc gaatcggtct
     3961 ccagaagtcg tcgaaatccg cgatgtggtc gcggatttcg tccgtgatgg ccgccgtgtt
     4021 cgccgcggtt ttcgagctgt cgtccgcggc gttcgcgaga tcctgagtca ccttgtactg
     4081 gcgctctaac agggagatca gggtgtcgag gtaaccggtg atcttcttga tgtcggcgat
     4141 ccggcccttc aggtagctga ggttgtcgcg gatgggcgcg ctctgcacac tgagctggaa
     4201 cggaatggat ccgtcttgaa tcggcgggcc aaggggtctg gtgatgctct gcacccgttc
     4261 gatgccgggc acgtggaaga gggcgctggc aatcttgtcc aggaccagca tgtcggtggg
     4321 attgcgcaga ttgtggtccg attcgatcat cagcaggtcc gggttcagac gggcctgggt
     4381 gaaatgttga tcggccgcgg tatacgcctg aattgaggat gcactcttcg gtaggtagta
     4441 caggtcgttg tagcgcgggt tgaagcccat caagcccagg gcgccgacaa tcgccaggac
     4501 gacggaggtg gcgaggatgg gtgccggcca ccgcacgatg gccgtcgcca ggcggcgcca
     4561 ccgggtgtag ttgaactttc gcttcgggtc gaatagaccg aagccgcttg cgaatacgat
     4621 cagtgccggt gtcagggtca ccccggcggc caccaccacc agcagaccga ttgcgcacgg
     4681 aaacgctagc gaactgaagt agggcagccg agtgaaatgc agacaggcca gtgccccgac
     4741 gatcgtcaga cccgagccca gcaccacctt ggtgacaccg gtgaaggtgt cgtagtaggc
     4801 ggcttcgcgg tcgagtcccc gctcgcgacc ctcctggtag cggcccacca tgaatatcgc
     4861 gtagtcggtc ccggccgcga tggccagtgc cgtcagcaag ttcacggcaa aggtcgaaag
     4921 ccccatgatg ccggtgtcgc ccagcagcgc taccacgcct cggccggtgg ccagtccgac
     4981 gaacaggatg accatcgtca gcagcacagt gccgatggac cgatagacga acatcagcat
     5041 gatcgcgatg atgacgatgg tgatgatcgt catcttgaac aggctcgcgt tacccacaac
     5101 gatcgtgtcg tcggtcaggg ccccctgacc cgcgacgtag gccttcaccc ccggcggcgg
     5161 cttcgagtcc gccacgatct tgcgaaccga gtcaacagac ttatcaccga ccgcaccgcc
     5221 ctggtcaccg cgcaggttga cctggatgta ggcggacttg tggtcatagc tctcgacacc
     5281 ggaggcggta aaccgctgcc cccagaagtc gagcgtatgt tccacgtgtt gggtgtcctg
     5341 cttcagcttc ttgaccagat cgtcgtaata ctggtgagcg tcttggccga gaggtctgtc
     5401 accgaccagg gtcaccagga cgaggttgtc ggagtcgtac tccttgaatt tctccccgat
     5461 gtgcttcatc ccggtcaccg aaggcgcgtc ggagggcgag agcggcactg agacatcctc
     5521 ggcaaccttc tccaactgtg ggacgaagac gttgacgccg accgcgatca gcagccagat
     5581 cacgacgatc ggcagcgcga ggacccggat ggtgcgggcc acacgcggtt tgcgctcaac
     5641 ttcggtgcgc ggcgcgacgg ggatttcgtc cgtgtcgctg gtgtcggtgg tgtcgctggt
     5701 cacgcggact tgtccaagca ggagacctgc gcgtcccgcg tatttacctg ttgctgatcg
     5761 accaatttgc cgtcgacggt gatccggcag ccgatggagg ggccgtcacc ttgggcgacg
     5821 acgtaggcga agatgccggg catctcggcg actatcgttt gcgaccacgg caacgtcgtg
     5881 aagttggcct tctgcggttg tgcgtccgca tccatccagt tgacgacgcc ggtcgtcccg
     5941 ggcggtccca ggatctcgta gatggcggtc ttggccgcgt acggcggggt cgcatcccgg
     6001 ggatcgggct taggcacgct gggtccgggg aagacgccgt tgagccggtc gactgcaact
     6061 ccgccgatga tcagtgcgac gaccacgacc aacggaaccc atgcccgctt aagcagctta
     6121 aacaccgtgc ctcccgatta ggtacgcccg aagggctgat atttagacat cgcgtcgcgt
     6181 ccgccaccgc tagccgaacg cctcagctgc tgagttaatt cctgcggttt gccgtcgcgt
     6241 cggagccacg cctccgcccg gcccgcggcc gcaccgctcg tcgtgttcca cccgatcagt
     6301 ttgctagcca cgacgtgacg ccggggtggg ctactcgcct gcgggcagca caaaagcaac
     6361 gcttgagagg catacgcacg accgtgttgt gtggctcgga gaggactatt gagtcgtaga
     6421 catggacaca ttgtgcgcta caagcgattt ggcgatgagg cgttgatctt ctgcccgttg
     6481 acctgttgcc cgtcgatctc attgatacgg ccgcgcggtc gtgtcgatct gcggcgacta
     6541 gcgagaatcg acgaaggctc atttactttg agcgagcaat tacttcgctg gcgcggcgac
     6601 gttactttga attgcacacg cgtgttttgc cagacgtcga ttgttcgccg tcgatctgaa
     6661 accagcaccg atttgggcca tggcgcgtac cctgacctgc atggctacca agaccaaggt
     6721 caagatcaag acctgcgtac attgcggcca cacgttcgat ccgaacgacc gacggcgtga
     6781 cctgtccgat ccggagtggc tcccggacgc atcagtttat tgttcgcatg aatgcagtga
     6841 tcgataccat tgcgagatgg ggcactgctg ctcgacccac gtgaaagaga aggctcggcg
     6901 cgaagccgcc aaggcggcgc agcccaaaac gtaggtcgat cggcgtcgat tcagtacctt
     6961 gccccgcgct aggacgcgaa tggaagctcg tcgtgttcgg gtggtcagcg ggcactacct
     7021 acgctgtcgg tcaagatgat tcagcttcgc ccgcgccctt gctctaatcc ggtcgccgct
     7081 cagcctcatc taacattcgc cgcgccctga atcctgttca tcgaacttag cgggctctga
     7141 ccaggcagcg tgccggctgg gatccggaat cacacgataa gcaaggctaa cgttcacaaa
     7201 tccattcaca agtcgcatgg aaatggcccc cggagaagtc cgcagacgtc tccaagggcc
     7261 atttccgctg gtagcgggga caggattcga acctgcgacc tctgggttat gagcccagcg
     7321 agctaccgag ctgctccacc ccgcgtcggt aaagacaagg ttaccgaaac ctcccaacca
     7381 cgccaaatcg cggctcagcg gcggcaacgg gcctggtcaa ccgaccgaca caattacaca
     7441 tccggcagtc ggccgccaag ctgccgcatc gtcggcagtt cccgcggcgt cgttcgtggc
     7501 tcggcgcgag cttcttgtcg tactcagttc gttctctcca ccgcatcggg ctcttcgtgg
     7561 aagccggcgg cgtccggtcc gactccgttg aacgcgatcg ggtccggatc gtccttccac
     7621 cgcttgatca ggtcgtgttc aattccgagt tgatcgatga cgcggccgac gacgtgggtc
     7681 gtcacctcgt cgacggagct gggtcgcgcg tagtaggcga tggtcggcgg gaagatcacc
     7741 gcgccggcgc gcgcgagata atgcatgttc tccaaatgga tctcgctcag tggagcctcc
     7801 cgcggcacca ggacaagcct gcggcgttcc ttgagcgtga cgtcggcggc gcgggtgatc
     7861 aggttgtcgc tgtaaccaac ccggatggcg ctcagcgtct tcatgctgca tgggcagacg
     7921 accatgccat ccgtgcggaa cgacccactg gagattcccg cggcaaggtc gtgggcgccg
     7981 taggcgtggg ttgccagcga tcggacatcc tcgacggtgt gatcggtctc gagcttgatc
     8041 gtcgcccgcg cccaatcgct gaggatcaga tgcgtctcga cctccagctc ccgaagagct
     8101 tccagcagac ggatgcccag cgcggcgccc gtcgctccgg tcatcgcgac gaccaaacgc
     8161 atgggtttct cctctcagcc ggccgtctcc aacagcagtt tcgttgcggc gcagctgatt
     8221 tggcgtgcat cgaacttaaa gacgttcccg ttcaacgcgg ctgtccgcgc cggtcggccg
     8281 gccctggcat cccgcgcagt agacatctgg actcctccca ctcgacgtcc gacgaatcgg
     8341 gcggcaactt cgccgaaatc ccttaggctg tgccgatttc agacttcaac agccatgccg
     8401 ctttctccag gtcaccgatg atgacgtgca gcaggtcggc cgtcgaggca tcggccgcgt
     8461 cgacgtcatc gtgcacggtc cgcatagtgt caacgacagc gtaagtccgc tcggtgatca
     8521 tgtcgacggc caccgccgcc cctacgagac ccggcggtat ggccggaacg gttgtggtcg
     8581 caacgaccgt gtccgagcgc ccatcgggaa cagcatccag ggcgcgcatg cgctcggcga
     8641 tggtgtcgct ggcctcgcgt gcgatgtcga cgatgctgtc gagctgcaga tgcaggtcac
     8701 ggaagttggt ccccacgatg ttccagtgca cttgcttgcc ttgcagatgt aactcgatca
     8761 agtcgacgag caccttttgc agattctcga acaggtttgc cggcgcgtga aacgacacga
     8821 tgtctgcggc gtcacggcgg ttggcggaca gcgaaacggt catgatgggc tccttatgtc
     8881 atgggatggc ggtggttggg gctggttgtc ctattcgggc caggtgttgt tgaggatgat
     8941 ctcgacgaag tcgtcgcgca cgaacgtggg gtcgtagtgc tgcagtacgt cggcgttcac
     9001 tgtgccaaag gtgctgcgcg ggcggtgctt catcccgtcg gtgaaggctc ccaggatgcg
     9061 ctgcttgaaa ttcgggcgcg ggtgggcagc ggtgaccgcg gccagagcat ccggagaaag
     9121 gtcttcgcgt ccaatgccca gcacgtcggt ttcgacgccc gctgtgacca aagcgatttc
     9181 cggcgccagg aattcgggca cgcccggcgt ggtgtgtagc gcgataccca gccacacctt
     9241 ctcggcgtcg gccttgtcca cgccgtgctg cagcagaaag tcgcgcgcgg cgttggcgct
     9301 gtcgacctcg aagcgccggg gcgaggttcg gtagcgctcg gtcaacccca ggtcgtggaa
     9361 catcgccccg acgtagagca gctcgaggtt tggttcgagc ccgaggcggc gaccctgcag
     9421 ggccccgaag aggaacaccc gccgggaatg gtcgaagagc aagtcgtttt cggcgtcacg
     9481 gatcaggtcg gtggcctcgc ggaccagagc ggtgtcaggg atcgcgatgc cggcaatggt
     9541 gtcgatcgac tgggcggtca ttttcaatct cctttggtga gctgatgcca gtctgcgggt
     9601 gagcggggcg atcagcccat ggctaatctg ccgtccttcc cacaaaatgc gacatggccg
     9661 ccttgtcccc aacgcggccg ggcgacacga tgagcacgtg gccacagcgg aaaagactga
     9721 gcgggtggtg gtgatcgtcg tcttcgacgg cgcgaaactg ctggatgcag ccgggcccgc
     9781 cgaggtcttc gccgaagcca accggtacgg cgcaaactat gcgatccaga tcgcttcggt
     9841 tgacgggcgc gacgtgacga cctcgatcgg cacacggttc gccgtcacgt gtgccatttc
     9901 gtcgatcgat tcggccgaca ccgtggtggt caccggcggc gacgatctga tcgggcggcc
     9961 gatcgatccg gctctggtcg aggcgctgaa aaccgtgccg ggccgaaccc gacggctggc
    10021 gtcggtgtgc accggctcgt tcatcttggc gcaggccggg ctgctcgccg ggcggcgcgc
    10081 caccacacat tggaaacaca ccagcctgct ggcccgcgcg ttccccgatg taggcgtcga
    10141 accggacgcg atctatgtcc gcgacggcga cgtgttcacc tcggcggggg tgacctcggg
    10201 catcgacctc gcgttagcgc tggtcgaatc ggattacggc ccggcgttgg tccgtgatgt
    10261 ggcacggtcg ctggtggtgt acctcaaacg cgccggcgga caatcgcaat tctcggtgct
    10321 ggtcgaggcg gcccctccgc cgggttcggc gcttcgcacg gtcaccgacg cgatcaccgc
    10381 cgatcccggc gccgaccaca gcgtgagaaa gctcgcggat cgggcatcgc tgagtacgcg
    10441 tcagctgacc cggctatttc agaccgagct gggcaccact ccggcgcgct acgtcgaaat
    10501 ggtccgcatc gatttcgcgc gcgcggcact cgacgcgggc cgcggcgtca acgaaaccgc
    10561 gggtctggcg ggcttcggca gcgccgaaac actgcgacgg gttttcgtca accacatcgg
    10621 catcagtccg aaggcctacc gggaccggtt ccgcaccgct tcccgcaact gataacgccg
    10681 cgcttacttg gtgttgttgt acttcgtgat ggcgtcgtcg agtcgctgta gcgctgaacc
    10741 gtacgcggcg aagtcgccct ttttctgggc gtcgcgggcg gcaccgatgg ccgcctggat
    10801 ctcttgcagc gcagcggctt tcgcgggcga caacgtgacc gcgccgtcgg gagccggtgg
    10861 cgccgccacg ggctccggcg gcgatcccgg cgcggcggcg ggggccggtg cgcccggggg
    10921 cgcgttcgct ggcggggcgg cctcggtggg ttgtatcccg gtggccgccg cgcccgcgcc
    10981 gggcccgaac agcccggtca gggcatcacc caccgtgggg ccgtacccga tcttgtcgtt
    11041 gtacatcatg gccacccgga tcagccgcgg gtaggacgac gccgcgtcgc tggctcccgg
    11101 ggaggcatac acgggttcga cgtagagcag tccgccctga ccgacgggca gggtgagcaa
    11161 gttgccccac cggatccggt tctgattgtc gcgcccgatg acaccgaggt cctgggacac
    11221 cgcgggatcg gtggtgatcg cgttattggc gagcttggga ccgttgacct gaccggggat
    11281 ggtcagcacg gtgatcttgc cgtacgtcgc ggggtcggag ctggcgctga tgtaggcggc
    11341 cagatagtcg cgcttgaacc tgttcatcgc gctgattaac tggtaggagg ccgagttgtc
    11401 ttgcttggca atgtttttcg cgacgatgta gtacggcggc tgatagctgc tggcggtcgg
    11461 gttcggatcc agcggtacat cccagaaatc agaggtggag aagaacgtca cggggtcgtt
    11521 gacgtggtat ttggccagca gcatccgctg caccttgaac aggtcttcgg gataacgcag
    11581 atgttcggcg agctcgggtg tgatgtcgct cttgggtttg acggtgccgg ggaagacctg
    11641 catccaggct ttcagcaccg gatcctgttc gtcctgctgg tacagcgtga cggtgccgtc
    11701 gtaggcgtcc accgtggcct tcaccgaatt tcggatgtag gagacctgct tgtcgggcgc
    11761 cagccggttg aacgccacct cggtggagtc ggcggtggcc gattccagcg aggtgagttc
    11821 ggagtacggg tagttgtcca gcgtggtgta gccgtcgatg atccacacca gtcgcttgtt
    11881 gacgatcgcg gggtacacgg cgctgtcagt cgttagccac ggcgctaccg cctccactcg
    11941 ctgcgccgga tcacggttga acaggatctt gctgttggag ccgatcacgt tggagaacaa
    12001 gaagtttcgt tcggcgaact tggcggcgaa gacgctgcgg gatatccagc tgccgaccgg
    12061 aactcccccc tgcccggtgt aggtgtagtt cttggtttcg gtgttggtct cgtagtcgta
    12121 ttcgcggtcg ttgccgttgc gtccgacgat cgcgtagtcg gcagacgtgt tggacatgac
    12181 gggcccgaaa tagatgcgcg gctggtctaa gggcgccggc ccgtcggata ccacgctgcc
    12241 gttggcgccg acgacgttga cgagaaactc cgggtagcca ccgttctggg tggggtcgtt
    12301 ggcgatgccc cgcacggtgt tggccggcga tgcgatgaat ccgttgccgt gggtgtagac
    12361 ggtgtgccgg ttgatccagt cgcgttggtt gtcgatcagc cggtcggggt tgagttcgcg
    12421 ggccgcgacg acgtagtcac gcaatgcacc gttgcggtcg aggtagcggt cgatggatag
    12481 ctggtcggga aagtagtaga agttcttgcc ctgctcgaac tgggtgaacg tcgggctgat
    12541 gatggtgggg tcgagcagcc ggatgttgga ggtggtcgcg cggtcggagg cgacctgctg
    12601 ggcggtggcc tgcccgtcgc cggtgtaatt gcgataggtg accacgtcgg acgtcaggcc
    12661 atacgcttgt cgcgtcgcgg tgatgcttcg gctgatgtat tcgctttcct tttgcgccgc
    12721 gttgggtctc acgctgatct gctcgacgat cagcggccac ccggcgccca cgatcaacga
    12781 cgacagcagc agcagcacca ggccgatggc cggaatccgc aagtcccgca aggtgatcgc
    12841 ggagaacacc gcggctgcgc agatcagggc gatcgccatc aggatcagct tcgccggcag
    12901 cacggcgttg atgtcggtgt acccggctcc ggtgaacggc ttgccgccgc gggtgtgcga
    12961 aagcagctcg tagcggtcca gccaataggc gagggctttg agcaacacca atattccgac
    13021 caggctgacc agttggatcc gcgccgagcg gctcagcgca ccggtgcggc cggacagccg
    13081 aatgccgccg aagacatact gcgccaacag gttcgccacg agggccagga aggccgcgac
    13141 gaacatgtag gtgagcacga gccggtacaa cggcaggtca aacgcgtaga agccgaggtc
    13201 cttgccgaac tggggatcct tgactccgaa ttctccgcca tgcaaaaaca gctggatccg
    13261 cacccaatag ctctgcccga cgatgcctgc cagcagcccg atcgccacgg gaatcccgat
    13321 gccgaacagc cgcagccgtg acatcacgac ggcacggtaa cgcgccacgg gatcgttggt
    13381 gctgctcggg acgaagacgg ggcgggtgcg gtaggccaac gcaagcgcgg cgaaaacgac
    13441 accgccgacc aggacgccgg cgaccaggaa gaccacgagg cgggtaacca gcacggtggt
    13501 gaacaccgag cggtagccaa gctcaccgaa ccacagccag tcgacgtacg cgtcgatcag
    13561 ccgcgggccg gcgagcagca agacgatcac acccagtgcg atcaggatca gagtccggct
    13621 acgccgagtc agcttcggca tccttgcggt gggccgcatc cccactggct acgctcctga
    13681 tcgatctgtc cggcgccggt gtggccgggc gccgttcggc cggatgactc ccggccggtt
    13741 tgtggtggcc acagaatttt tctggacgtc acaactgtac gcacttgcgg cgcgcgggat
    13801 cgcgggccag ttcgctgccg tctgtgcctg atcgcccacc ttctcctcag cccactgcgc
    13861 gggctgcatc gtcggcgggc cgtgcctgat cgcccacctt ctcctcagcc cgtccccctc
    13921 gccgctccgc ggctggggca ggctagcaac tgggcggctg accccccgac gtgatcgcgt
    13981 gcaacgcgtc caccgcttcg ctgagggtct cgaccttcac caagcgcaac ccggacgggt
    14041 tgtccgagga cgcctcgtag cagttcttgg cgggcaccag aaacactgtg gccccggcgt
    14101 cgtgggcggc gaccatcttg tgggtgatac cgccgatttg gcccaccttg ccgtcgatgg
    14161 tgatggtgcc ggtgcccgcg atgaacgtcg acccggccaa gtcacccgtg gtgagcttgt
    14221 cgacgaccgc caggctgaac atcaggccgg cagacggtcc gcccacgttg gcgaggttga
    14281 aatcgacgac gaacggcgcc cagggcgcgt ccagcaccgc gactcccatg aagccgtagt
    14341 cgcgatcttt gttcgtcccc agggtgatct gggccacgcc ggccggttcg ttcttacggc
    14401 gatagtcgat ggtcaccacc tggccgggtt tggtgttctt cagcaatccc gtgaactgct
    14461 ccacagtgct caccggggtg ccgtctaccg cgtcgagagc atcgccttcc ttcagcttgc
    14521 ccgctgacgg gccgggatcg ctgaccgtcg cgacggtcac cgcgggcgcg tacttcaaat
    14581 atcccagcgc ggcgtactcg gcactgtcct cggagttctt gaagtcggcg ttgttggcct
    14641 tgtcgacgtc ctcccgcgac ttccccggcg gatacacgag gtcgcggggc accagctgtt
    14701 cctgtcctga cagccacagc gtcagtgctt ggcccagggt cagatcgtcg cgttgggaca
    14761 ccgtcgtcat gttgaggtgc cctgttgtcg ggtgggtctg ggtgccttcg atcgcgacga
    14821 cctgcttgcc gtcgacctca ccgagcgtgt cgaaggtcgg gccggggccc agcgacacaa
    14881 acggcaccgt caccaccgcg agcaggacgc cgaaagccag aatcggcacc agcgcgacga
    14941 tcaaggtcag aatccgcctg ttcacgccgt ttacactaga cggaactcgt tcgggctcat
    15001 tcagctacga gcatgacgcg agattacgcg ttctgttccc tcggtgagta ccgttgatgt
    15061 catggctgac ctgcctttcg gcttctccgg gggaaacgac cccgagcgcg acaagcacgg
    15121 caagaaagac cccgatcccg gctctggccc gtccgaccca ttgggcgcgt tcggcatcgg
    15181 cggcgatttc ggcatgggtg acttggggca gatcttcacc cagctgggtc agatgttcag
    15241 caacgcgggc accgtgacag ccgggggcaa atccaccgga cccgtcaact acgacttggc
    15301 gcgccaagtc gcgtccaatt cgatcgggtt cgtcgcgcct atcccggccg ccacgaacca
    15361 agcgatcgcc gatgcggtcc acctggccga aacctggctg gacggggcaa cggcgctgcc
    15421 tgcgggcacc accaaggcgg tgggctggag cccaaccgac tgggtggaca acaccctgga
    15481 gacctggaaa cggctgtgcg atccgatggc cgagcagatt gccacggtgt gggcgtcctc
    15541 cctgcccgaa gaggctacga gcatggccgg gccgctgctg gcgatgatgt cgcagatggg
    15601 cggcatggcg ttcggatcgc agctgggcca ggcgctgggt cggctgtccc gcgaggtcct
    15661 gacctcgacg gatatcggtc tgccgctggg ccccaaaggt gtggcggcga tcatgccggg
    15721 tgccgtcgag gcgttcgctg ccggactcga gcagccgcgc agcgagatcc tgaccttcct
    15781 cgccgcccgt gaggccgcac accatcggct gttcggacac gtgccctggc tcgccagtca
    15841 actgctgggc gccgtggagg cctacgccat gggcatgaag atcgacatga gcggaatcga
    15901 ggagctcgca cgcgacttca atcccgcgtc gctggccgat ccggcggcta tcgaaaacct
    15961 gctgggtcag ggagtgttcg agccgaaggc cacgccggcg cagcagcagg cgctggagcg
    16021 gctcgagacc ctgctggcct tggtggaagg ctgggtgcag gtggtggtga ccgcggccct
    16081 gggcgatcgg atcccgggca ccaccgcgct cagcgagacg ctgcggcggc gccgggccag
    16141 cggcgggccg gccgagcaga cgtttgccac gctggtcggc ctcgagctgc ggccacgcaa
    16201 gctgcgcgaa gccgccgcgc tgtgggagcg gctgacccag gcagccgggg cggacgcccg
    16261 cgacgccatc tggcaacacc cggacctgct gccgtccacg gacgacctcg acgagccggc
    16321 cggcttcatc gaccgcgtca tcggcggaga caccagcggt atcgacgagg cgatcgccaa
    16381 actcgaggaa tgcgccgaca cccaggaccg cgactcaggc ccggacggcg gtcctgtgga
    16441 taactgagcg cggcgttcgc ggccggcgtg gcacagttca accgtgtcgt cggccgtggc
    16501 ttccagctat gcgctcgatc cggcgctgcc ggtgttgctg cgacccgacg gtgctgtcca
    16561 ggttggttgg gatccgcgcc gcgccgtgct ggtccgccca ccgggcggcc tgcccgccgc
    16621 cgagttggcc gcactgctgc ggtccatgcg gtcaccgaca ccgatagccg aactgcagcg
    16681 gcgggcgggc gaccgattgc aggacgccga cggcctgacg aatctcgtcg cgcaactggt
    16741 cggcgccggc gtcgcgaccg aatgtgggcg gtcgcgcggc cgggcggcgt cgatccgcat
    16801 ccacggccgc gggccgctgt cggacctgct cgtcgaggcg ctgcgctgct cgggggcccg
    16861 catcgcgcac agcagccaac cgcacgccgc ggtgtcgccc accgaggtcg atctggtggt
    16921 gttgtcggac tatctggtcg ccgacccgcg catggtgcgc gatctgcaca gccggggcgt
    16981 cgcgcacctg ccggtgcggg tacgcgacgg caccggcctg gtgggcccgc tggtcattcc
    17041 cggcgtgacc agctgtttgg ggtgtgctga tctgcaccgc agcgaccgcg acgccgcatg
    17101 gcctgccatc gccgcccagc tgcgcgacac cgtcggggtg gccgaccggg ccacgctgtt
    17161 ggcgaccgcc gccctggccc tcagtcaagt gaaccgggtg atcgcggcgg tgcgggggca
    17221 ggcgggtgtg cccgaccccg cgccgccgcc ggcgctgaat gccaccctgg agttcgacct
    17281 caacgcgggc tccatcgtcg cacgtcagtg gaccaaacac ccgttgtgtt catgctgacc
    17341 gcgcgtggcg ggtgcctagc ccatccgtcc ctggtcgggc acgttcgtcg ccaccgacgc
    17401 atatgccctg gagagctcct ccaggacccc cgcagtcagg gtccccgcgg tccggttctt
    17461 gtccagcgtt tcgatgatcg ccctgaacaa tgcgtcggcc gcatcgtgct gcgtctgctt
    17521 ggctgccatg gctttttcgg taccaccctt cggatgcgcg tgtcggtgag gcgctcagct
    17581 accacatgca agggacaact tatggcccgc cggccaggtc cgccgagtga tacaacacgc
    17641 ttaacacccc tgcaatggtt tgtcggcaga tacaacgcgg cgtcctgacc ctcatggatg
    17701 atggatgtgt ggcagacatc aaacgcggcc gtgctgcgcg taacgcgaag ctggccagca
    17761 ttcccgtcgg cttcgccggt cgggccgccc tcggattcgg taaacggtta accggaaagt
    17821 ccagggacga agtccaagcc gagctgctgg agaaggccgc caaccagctg ttcaccgtcc
    17881 tgggtgaact caaaggcgga gcgatgaagg tgggacaggc gctttcggtg atggaagccg
    17941 ccattcccga ggaatacggt gagccctacc gcgaagcatt gaccaagctg cagaaagacg
    18001 ccccgccgct gcccgcccac aaggtgcatc gggtgctcga cgcacagctg ggcaccaagt
    18061 ggcgggaccg gttcggttcc ttcgacgaca ccccggtcgc ctcggccagc atcgggcaag
    18121 tgcacaaggc ggtgtggtcg gacggccgcg aggtggccgt caagatccag tacccgggag
    18181 ccgacgaggc gctgcgcgcc gacctcaaga ccatgcagcg gatggtcagc gtactcaggc
    18241 agctctctcc cggcgccgac gtccagggcg tggtcgatga actcatccag cgcaccgaga
    18301 tggaactcga ctatcggctg gaggccgaga accagcgcgc gttcgccaag gcatatcacg
    18361 gccacccgca cttcgcggtg ccccgcgtcg tggccagcgc gcccaaggtg gtgatccagg
    18421 agtggatcca gggcgtgccg atggcggaga tcatccgtga cggcacccgc gcggaccgcg
    18481 acctgatcgg cacccggctc ctcgagctca ccttcgacgc gccacgtcgg ctgggaatga
    18541 tgcacggaga cgcccacccg ggcaatttca tgctgctgcc cgacggccgg atgggcgtca
    18601 tcgacttcgg cgccgtcgcg ccactgcccg ggggctatcc catcgagctc ggcatgatca
    18661 tgcgcttggc ccgggagaag aactacgacc tgctgttgcc gacgatggag aaggccggct
    18721 tcattcagag aggccgacag gtgtcggtgc gcgacatcga tgagatgctg cgccaatacg
    18781 tcgagcccat cgaggtcgac gtcttccact acacccgcaa gtggctgcag cgaatgaccg
    18841 tcagccagat cgaccggtcg gtgtcgcaga tcaggacggc gcggcagctg gacctacccg
    18901 tcaagctggc gattccgatg cgggtgatct cgtcggtggc cgccatcctg tgccagttgg
    18961 acgcgcacgt gccgatcagg gcgctgtcgg aggaactgat ccccggtttc gccgaacccg
    19021 acaccgccgt cgtttagggc cggcccggcc cggcgggtca ggcggcgacg gggctttgct
    19081 gacccttgcg gggacgtccg cgtggacgtt tgcggctgac gatcgaacct cgctcgagaa
    19141 tctcgccgcc ccacacaccc cagggttcgg cgcgttccag cgcggccgtc aaacactggc
    19201 ggcggatcgg gcagtccgcg cagagcgtct tggcgcgctc gaggtcggct ggggcctcgg
    19261 cgaaccacag gtcggggtca tcggcgtgac acggcaactc gggcagcgtc ggtttgggga
    19321 ctggcggtgc cgacatgtcc tgctcacctg cttcctggtc ggttggttgt ccttcgcagt
    19381 gatccggacc aggttgggag gctgtcgacc tgacaactat ggccacggat cctggtgact
    19441 ggggtccgtg gccatctggg taaaaccggg cggttacctt gcgctcaggt atggccccga
    19501 tccacggacg cgtcagtcgc ggcggcgcga tgctttaccg cggccgccgg cgcggcggtg
    19561 cgggcggcat ggaaggtcgc cgcggtcagc accgcggcag ccacgcctgc accgaacgtg
    19621 ttggtgatca tggtggcgac cctcctctcg tcttgtccgc ccgccgaccg gccctcagcc
    19681 ggcagcagaa tgcctttcga gggtaaatgg tagcggccga tggccacaac cgattttctg
    19741 acctgcgctt tcgccgctca acgggcttgc ctgaccaact ccagcacgtc gggtccgtac
    19801 tgttcgagct tgcgcgcgcc gatacccggg atcgcgatca gggccgtttc gtcgtcgggc
    19861 agcaactcgg cgatggcgat cagcgtgttg tcggtgaaga cgacgtacgc gggcaccttc
    19921 tgttccttgg cgacgtccat ccgccacgcc ttgagccgca gcaacagctc ctcgtcgatg
    19981 tcggcggcac atgtttcgca gcgccgcagc atgatggccg ccggcgtggt caggttgttg
    20041 ttgcagaccc ggcagcggct tgcggtgccc cggttgcggc gcggccggtt cggcgccggg
    20101 tcggggcggg tctgcggcgc gatgccgttg aggaaccgcg acggtttgcg gctctggcgc
    20161 ccgcccgggc tgcgtgccaa ggcccagctg agcgccaaat ggactcgcgc cctggtgatt
    20221 ccgacgtaga gcagtcgacg ctcttcctca acggcctcgc tgtcggcgcc gtgcgccaac
    20281 gcgtgcgaga tgggcaacgt gccgtcggcc accccgacca agaacaccgc atcccactcc
    20341 agtcccttgg cggcgtgcag cgaagccagc gtgacgccct gtaccaccgg cgggtgccgt
    20401 gcgtcggcac gcgtccggag ctcggccagc agccccgcga aatcgagctc ggggcgctgc
    20461 gccacttctt cgtcgaccag ttcggccagc gaactcagcg cctcccagcg gtccagggcg
    20521 cgggtgccgg ccggttgctc ggccgtcagc cccaggggct ccaggacggc gcgcaccacg
    20581 tcgggcaacg gcccggtcga gggttgctcg gaggctcgtt ctgaggcgcg ctgcagcgcc
    20641 aacagcgcct gcttgatttc ctgacggttg aaaaatcctt cgccgccgcg aatttggtag
    20701 gtgatgcccg cctcggtcaa cgcttcctcg tagacctcgg attgcgcgtt gacccggtag
    20761 agcaccgcga tctcggatgc cgcggtgccg gattcgatca gccggccgat cgacgccgca
    20821 accgcggccg cctcggcggt ttcgtcggga tgctcgtgaa acgacggggc cgggcccggc
    20881 gcgcgctggc ccgacaactg aagcttgctg ccggcgaccc ggccacgggc cgcggcgatc
    20941 acctggttgg ccagggaaac cacctgcggg gtggatcggt agtcgcgttc cagccgaacc
    21001 accgtggcat ccggaaactg ccgagagaag tcgagcagga agcgaggcga ggccccggtg
    21061 aacgagtaga tggtctggtt ggcgtcaccg acgacggtca ggtcgtcccg atcgcccagc
    21121 caggcagcaa gcacacgctg ctgcagcggc gtgacgtcct ggtattcgtc gacgacaaag
    21181 cagcggtagc gatcccggaa ctcctccgcg acggccgcgt cgttctcgat ggcggccgcg
    21241 gtatgcagca gcaggtcatc gaagtcgagc agggtcaccg attcgtcgcg gaccttgagg
    21301 gcctcgtagg cggtgtagac ggtcgcgatc tgcgcggcgt ccatggggat gtcccggccg
    21361 gcggcggcca cggcagtcgg gtattgctcg gggccgatca gcgacgcctt cgcccactcg
    21421 atttcaccgg ccaagtcgcg cacgtcatcg gtgctgacgt tcagcctggc gcggctggcc
    21481 gccctggcca cgacggcgaa cttgctgtcg agcagctgcc accccgtgtc gccgaccact
    21541 cgcggccaga agtaccgcag ctgacggtgc gcggcggcgt ggaaggtcag ggcctgcacc
    21601 gtgccaatcc cggaaccggt gtgcgcggtc gcggcgagtc ttcgcagccg ggaccgcatc
    21661 tcccccgcgg cgcgctgggt gaacgtgacc gcgagcacct gcccggcggc gacgtgacca
    21721 ctcgcaatga gctgcgcgat ccgatgcgtg atggtgcggg tcttgcccgt tccggcgccc
    21781 gcgagcacgc agaccggtcc ccgcggggcc agcacagctt cgcgctgttc gtcgtccagc
    21841 ccagcggtca atgcgtcagc gaccaagggc atggcgtcca tactggcagg agtggccgac
    21901 aaaccgggca cggcgccacg ccgattggcg tgcctggtgt tcggattcga cgcatcccgg
    21961 tgcacgcaat tcgttgtcgc aggtctacgt taacgggcta tgaccaacgc tgcgattacc
    22021 gtctacacga cgtcatggtg cgggttctgc catcggctca ggacggcgct caagtcggat
    22081 gccatcccct acgacgagat cgacatcgat catgacagcg ccgcggcgga gttcgtcagc
    22141 tcggtgaacg gcggcaacag gacggtcccg acggtgaagt tcgccgacgg gtcgacgctg
    22201 accaacccga gcgcggccga ggtgaaggcg aagcttgccg aggtcgccgg ctagtcgagt
    22261 gccgcccacg attcgatgat cacgcgtgcg atcgagatcg acccggggag cagcagtttc
    22321 gaccccgacg agctgctcca gtcgccggcc tccagcgctg cgcgcacctc agcgcgggtg
    22381 aaccagttcg cctcggcgat ctcaccgtcg ttgaacgaga agtcctcagc cgggtcagcc
    22441 tgggcatgga agccgaccat cagcgagcgc gggaacggcc acggctgact gcccaggtag
    22501 cgcacgtcgc gcacgtgcag cccgatctcc tcgcggatct cccgagccac acagacttcg
    22561 aaagactcac cggcctcgac gaatccggcc agcagcgaga acatccgctc gggccacacc
    22621 gtctggcgcg ccagaacggc acggtcgccg ccgtcgtgga ccagacagat caccgccggg
    22681 tcgatgcgcg ggaactcctc gtgaccggtg atcgggttga tccgcgacca gcccgccctg
    22741 gccggtctgg tcggcgcgcc gtccaccgcg ctgaatcgcg cgttgtcatg ccaattcagt
    22801 agcgcgatgg ccgacgacac cagttggctg ctggtgtcgt cgaagatcgg gccaaggctg
    22861 cgcaggttca ccacatcggc ttggacgtcg ggatcctccg gtgcctgcag cgcaccacgg
    22921 acagcccaga cgtggcggcc accctcgatg cggcccagaa ataccgcgtc cggcggcggc
    22981 ttgtgggcca gctcggccgc ggcgccgagc accgcccggc cgtcggccac cagcacctgg
    23041 ttgcgcgaat ccacccgcag cagcgccgcc tccgcccatc ccgcggcggc cgcatcgaca
    23101 tccgtacgca attgatcggc tcggtcggcg ccgacgcgcg acagcacggg aatatcgcgc
    23161 agctcgaagt ccacgaccta atgtcccgcg tgtcggatgt agagcagccg gtcgctggcc
    23221 tcgatggcgt ccacctcggg cgcaccgatc cgcagcaggt ggccgtcgcg caccacaccg
    23281 agcacgatgt cacgcagatg ccttggcgat ccgccgattt cggtctgttc gacctcgcgt
    23341 tcggcgattg ccaatcccgc gtccggggtc agcaggtcct cgatcatctg caccacgctt
    23401 ggcgtggtgg tggcgaggcc cagcagccgg ccggccgttt ccgacgagac cacgaccgag
    23461 ttcgcgcccg actgctgcaa caagtgctgg ttctcggcct cccggattga cgcgacgatc
    23521 ttggccttgg gcgcgatctc gcgcgccgtc agcgtgacca gcacggcggt gtcgtcgcgg
    23581 ctggtggcga cgacgatcga agccgcgtgc tgcgcaccgg ccagtcgcag cacatcggat
    23641 cgggtggcgt cgccgtgcac ggtgaccagg ccggcggtgg tggcgtgttc gaggacgttc
    23701 cgatcggtgt cgacgacgac gacctcaccc tgggcggcct cgtcaccgag gatggcggcg
    23761 atcgccgttt tacccttggt gccgtagccg atcacgatgg tgtggttgcg cactttgctc
    23821 ctccaacgct ggatcttcca cgcctgccgg gaccgctcgg agagcacctc gagtgtggtg
    23881 ccgaccagca cggccaagaa cgcgatccgc aacggcgtaa agatcacggt gtggacgaga
    23941 cgcgcggttt cggtgtacgg ggtgatgtcg ccgtaaccgg tcgtcgacag cgacaccgcc
    24001 gcaaagtaga cgcagtccaa aaatgtcggc ggtccgccgc gcacatcccg atagccggtg
    24061 cggtccagat agacgatgac ggcagcgccg aacaacacca ccaacgcgat agcgacccgg
    24121 cgcgtgatga cgcggaccgg gctgccgtgg tcgtcgggga tgcgcaggac accgacgagc
    24181 aggtgatcgg gctgggccgt cagcgtctcg tcgagaccgc tcagtttgcg tgacttaccg
    24241 ttgcccatgg cggtccgtca tcggcttgag cccagcgcac gagacacgca cggttcaaat
    24301 gtaaccacga tggcggttcg ccccatactt gggcagcgca cgtcacacct cggcggggtc
    24361 cgcctgaagc tcggccagct cagcggcatc gggtagtacg tcgggagcga ccgtggcgcc
    24421 ggtgcgcacg taatagaagg ccgcgcgcac cgacgattcc gggacccccg acaaagcggc
    24481 ccacgccaag cggtacacgg cgagctgaac ggccgcacgc cgcatggcct ccgccccgtg
    24541 cggcggctct ccggttttcc agtcgacgac ggtggcgccg ccgtcgggct cggcgaacac
    24601 cgcatcgatg cggccacgca ccacggtgtc gccgatcggc atctcgaacg gcacctcgac
    24661 ggcgacggga gtgcgcgccg cccaacgcga ttcgataaat gcggcctgca gtgcggccag
    24721 ttcccgggtg tcgccgacgt cagagtccgc cgcgcccggc aggtccgcga tatcgaacag
    24781 ccactcggcg ccgtagaact gctggaccca cgcgtgaaac gcgttgccca gcatggcatg
    24841 cggatccgga cgcgtcggca gccggcgcat cagccgctgc gccgcgccgg cggggtcacg
    24901 cgccaggtcg accagcgcgc tgaccgacaa ctggctgggc agggtacggg cggccggtcg
    24961 tggcgaccgg gcgcgctcgg ccagcaaggc atcgacgtcg gcggtccagc cgtcgacgtc
    25021 agcgccgggc gcagccgtgt cggccgacat cgccctggcc accagggccg ccccacgttc
    25081 gacatcgccg cgacgcgccg ccaacgggtc ggcgggccag accgcttcaa tgacattgtc
    25141 gtgcagtgga tttcgttcac cgtcggaggg cgcgggcgcc cactcgtcga ccatcccaca
    25201 cggagcgccg gcggcggccg cacggtcgac gatgtccttg agttcggcca ggaaatcaga
    25261 cgggccccgc ggcttgaccc ctgtcgcacc ccaatgatgg ccggacacca gcagagtgtc
    25321 ctcggcccgg gtgatggcca catacagcaa gcggcgttcc tcgtcgacgc ggcgctgctc
    25381 gagctgccgg cgatgttcgg cgatcttgtc cgacagctgc tttcgattgg tgacatccgc
    25441 ggtgtccagc actgggatgc ccagcgagcc cgccgaggcg cggtcaccgc gcagtagcgg
    25501 cggcagctcg gcggcgtcgg tgagccaact gctccgcgac gcggtcgacg gaaagatgcc
    25561 tcccgacagg tgcgccaccg cgaccacttg ccactccaga cccttcgcgg agtgcacggt
    25621 gagcaccggg acgcggtcgc gcgcgaccgt cgacggcgcg ggcgacaagc cgttctctac
    25681 cgcctcggcg acgtccaggt aggccagcag gccggccacc gaagtgtccg tcgcgcttgt
    25741 tcgttccccg tagccggcga cgacgtcggc gaacgcgtcg aggtgttccg cgccggacca
    25801 ggcccccaat cccgctgcgc cgggtgcagg gaccgtcgcc gcggcccgga cttcgcagtc
    25861 gacgcccagc acgcgtcgca cctcggcgac caagtcgggc agcgaatgcc ccaggtgacc
    25921 gcgcagggcg ctcagttcgc cggccaggga gccgatgcgc cgatacccca cggccgaata
    25981 ggagccggcc gggccgggat cgctgatcgc gtcggccaga cacgcggcgt ctgctccgat
    26041 gttggaggga ccggccgcca tcgccagcgc ctcaggtgac gacgcgtgac ccgagccctc
    26101 gccaagggcg agcgctcggc gccagagcgc gacgatgtcc ctgccgccga ggcgccaacg
    26161 cggaccggtc agcactcgca tcgccgccgc gcccgccgtc gggtcggcga ccaaccgcag
    26221 catggccacg acgtcggcca cctcggggat ggacagcagc ccggccagtc cgaccacctc
    26281 gacggggatg ccgcgcgcgc gaagggcgtc ggcgatgggg gcggcgtcgg cgttgcggcg
    26341 caccagcacc gcggccgtcg gtgggctgac gccgtcggcc tgagcacgct ggtactgccc
    26401 atacaagtgg tcggcgatcc actcgcgttc cgcccggaca tcggcaagca acgcgcagcg
    26461 gacggtgccg ggaggagcgt ccggtcgcgg gcgcaacgcg tggactgcga ccgatcgtcg
    26521 ccgcgcctcg gccgatatcg cgttggccac gtgcagggcg cgcggcgggt tgcgccagct
    26581 ggttcgcagc tccagaaccg gcgccggggt gccgtcggag tgggggaagt cggtgctgaa
    26641 ccggggcaga ttcgtcgccg aggcgccgcg ccacccgtag atcgactgaa tcgggtcgcc
    26701 gacggccgtc aacgccaatc cgtcgtcgac tccaccgccg aacagcgccg acagtgcgat
    26761 gcgctgggcg tgcccggtgt cctgatactc gtcgagcaac accacccggt agcggtttcg
    26821 cagctcctgg ccgacctggg ggaaggtcac cgccagccgt gcggcggatg ccatctgcgc
    26881 accgaagtcc atcaccttcg cggcgcgcat gcgttcccgc agcgcgtcga gcagcggcac
    26941 cagctcggtg cgctcggtct gggtggccag cagccgaagc aaccactggc tgggtccgcg
    27001 gtcgcgctgg aaggggcccg ccggcagggt gtggatcagc cgttccaact cgacgtgggt
    27061 atcgcggagc tggcgggtgt ccaccaggtg ctcggccagc tgaccccata gccgcagcac
    27121 catcgaggtg atcgcggccg gggtcttgtc ggtgcgcagc tccccgccgt acccgttgac
    27181 cacgtcgaac gccagctgcc acagctcggt ctcgctgagc agccgggtgt cgggctcgac
    27241 gggcagcaag agtccgtagt cgcgcagcag cgagccggcg aaggcgtggt aggtgctgac
    27301 taagggcgtg cccaccggtt cgagggcggc cgcggtggcg ggggtggtca ggtccagatc
    27361 gatgccggcc aaccgggcca gtcgggaccg gacccggcgc agcagctggc cggccgcctt
    27421 gcgggtgaac gtcaatccca gcacctggcc gggttcggcg tagccgttgg ccacgagcca
    27481 caccacccgc gcggccatcg tctccgtctt gccggcgccg gcccccgcga tgacgaccag
    27541 cgggcccggc ggcgcggcga tgacagccgc ctgctcggcc gtgggcggaa aaagccccag
    27601 cgcgccagcc aattcggccg gactgtagcg cggattcacg gcgccaccgt cccgccggat
    27661 ccggtgtggg ccgggcagca cggtcgtatc gggcagtgcg tgcaaccgtc gttgcggcgg
    27721 gcgatgaatt ggggccccgc ggtcgtgccg gctgccaacc gcacgaggtt gcgccattcg
    27781 tctctcgcgg ccgtggtcag cggatcctgt tcgcgcaccg tggcacccgc ggccccggtt
    27841 ttgcccagat agaccagccg cgcgccgccg ggctccgcac cgtcgccggg gccggcgttg
    27901 atcaagccgt cggcaattgc cagctggtac atcgccaact gcgcgtgttg ttgagcgtcg
    27961 tccttg
//
LOCUS       DASSGQ010000014        18931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_335505,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000014 DASSGQ010000000
VERSION     DASSGQ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18931
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335505"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1102)
                     /locus_tag="VFI55_00680"
     CDS             complement(173..1102)
                     /locus_tag="VFI55_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7072583.1"
                     /translation="MQWFTHHAVAIFSALLAAFWAAVGIVTRQRVAQDMPADQAMSEK
                     LLPSLLREPLWWAGTLAAVAGYVFQAVALAHGSLLLVQPLLVSSLLFALPLSARVCHQ
                     HISRADWGWALVLTAALSVFVIVGRPHEGHNRPPVQAWTLALVVIVPLVTVCVVAARR
                     ASGRVRAMLLAVAVAVLLGMIAVLTKICTHRFAVGGWHGLLTVPAPYVLVALAAILTV
                     VQQSAFHAGSLQASVPIMLVGEPMVAVLLGVVVLGEYLTVRGPAALGLAAAVMAMIVA
                     TVALARDEAPKSAGTAPPRQQPAPTTAVSGLER"
     gene            complement(1269..2873)
                     /locus_tag="VFI55_00685"
     CDS             complement(1269..2873)
                     /locus_tag="VFI55_00685"
                     /inference="COORDINATES: protein motif:HMM:NF038019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PecA family PE domain-processing aspartic
                     protease"
                     /protein_id="HET7072584.1"
                     /translation="MSFLVSPPEVISSLLYAGAGSGPMLAASAAWEGLAAELSSAAQS
                     FSSVTSGLTEEAWQGAASTAMLSTAAQYSGVLSVAAAQAQTAAAQAQAVASEFESALA
                     ATVHPALVSVNRNQLVQLVVSNLFGQNAPAIAAAESEYEQMWAQDVSAMVGYHGGVSA
                     AAAQLPTWAAAIKGLPGQAVAAAAGSPVGSVLNAAAASPIGAAQSTLNEAASAGALAD
                     PLGTLELYAIKAINAPTEILIGRPLIGGTSYALGGSANGTQATVPLTVVGGTEPIVNG
                     SVGTGTPQPLLVDTGSTGLVIPFQNAGGLLGVLQLGIPRGIGIGGYSGGLDYLYLTYN
                     APVNFGGGVATSPTPVDIEFFAWPVSIHSAIANGFTFQSFFSSDGATGVLGVGPNAGG
                     PGPSIATQALPAPYNQGVLINQPAGQLVFESQSSVGLPPIATLTGSPITTLDVSLDGG
                     ATKIPVSSIVDSGGVDGTLPTNLNAPVGTNISVYAPTGQLLYQYGLGTTYSPLLISSG
                     LMNTGNLPFEQHAAYIDYGANTTTFY"
     gene            3198..3959
                     /locus_tag="VFI55_00690"
     CDS             3198..3959
                     /locus_tag="VFI55_00690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7072585.1"
                     /translation="MTDRQERAMSFGSIAENYDGLRPHAPQQAVDWLVPSGCDVAVDV
                     GAGTGLFTRALAGKAAHVIAVEPDARMRAVLSARSSGVRVVEGHGESIPLPDASADAL
                     FVSSAWHWMDPERAVPEIGRVLRDGGRFGLIWTSRDREVDWVVGLDRLPGEEASEAER
                     FRRHLDVVLPEPQIFGDIARETFRFVRTMSVEDVIAMLGTYSRVIVASPQDRDRRLAQ
                     ARALLKERFPSADTIEVPMRARCWRADRIARGDGQ"
     gene            4016..4678
                     /locus_tag="VFI55_00695"
     CDS             4016..4678
                     /locus_tag="VFI55_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HET7072586.1"
                     /translation="MSALSIARIVGVSGMAAWALWSAPVHTPVASADPCTDVAVVYAR
                     GTHQAPGLADVGQAFVDSLTSQVGGRSLSAYAVNYPATDDYHGSAPAGADDASAHIQQ
                     TVAECPNSKIVLGGYSQGSTVIDLATTSMPAPVADHVAAVALFGEPSSGFSTALWGGQ
                     PLPTISPLYAGKTLSLCAPGDPICSAGGDIMAHVSYIQSGMTTQAATFAANKIGPGTP
                     NA"
     gene            4769..5419
                     /locus_tag="VFI55_00700"
     CDS             4769..5419
                     /locus_tag="VFI55_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HET7072587.1"
                     /translation="MKSLGALSFETVAVRIVVAVVIGVAVAIVVGNTAGWRFALVGWV
                     VTAGLYVVWTRLLLGGLDADETREYVTREDPTRWVADAVVVSASLASLGGVGYVVAAG
                     SHSGAGAIEAAILGILTVAASWFAVHTLFSVHYARLYYSDEPGGINFHDPEPPRFRDF
                     AYMAFTVGMTYQVSDTEIGLTSIRATVLRHAMLSYLLGAVVLAVTINLIAGLGSKL"
     gene            5462..6010
                     /locus_tag="VFI55_00705"
     CDS             5462..6010
                     /locus_tag="VFI55_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET7072588.1"
                     /translation="MAIESTMLALGTPAPSFSLPEPATGATVSLDELTGPALVVTFIC
                     NHCPYVKHVAAGLAALGRDLGELGVAMVGISSNDVVTYPQDGPDEMVAEARHHGWTFP
                     YLYDETQDVARAYSAACTPDTFVFDGERRLVYRGQLDDSRPKNGLPVTAADVRAAVDA
                     VLAGRPVDPNQRPSIGCGIKWR"
     gene            6082..7254
                     /locus_tag="VFI55_00710"
     CDS             6082..7254
                     /locus_tag="VFI55_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7072589.1"
                     /translation="MKVALAGYGSRGDVEPLAAVGRELLRRGHHVAMAAPPDQLGLVA
                     SAGLTSVEYGPGTQALRGQEFAPDTSPNPINTMVKIAQHFTQAMTDFGTTLTEQADGA
                     DLLLTNASEEGLATSVAEYYGIPLATLHFFPGAQPRGLIGDIAKDAENALRRQLGLPE
                     PTGRSAELEIQAYDEFWFPGLAAKWGEQGRRRPFVGTLTLELPTDVDAEVLSWIAQGT
                     PPIYFGFGSNVQIPYPDETIGVISAACAHLGERALICSGSSDFSWVTHADHVKIVTSV
                     NHGAVFPACRAVVHQGGAGTTAAGIRAGMPSLILWFSSEDQPTWAQAITRLKVGCGRQ
                     FSASTLATLVADLHSVLAPECAARAQELAPQMTKSAESVAKAADLVEETARLGRAD"
     gene            complement(7278..8132)
                     /locus_tag="VFI55_00715"
     CDS             complement(7278..8132)
                     /locus_tag="VFI55_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HET7072590.1"
                     /translation="MEKPTASAGLGEAQLRVQSLCRYPVKSMLGETVDSMFVDEHGAE
                     GDRRLALLDAQTGHVASAKQARLWRGLLKCTADGNTGRVRIALPDGTNVTADDPGVDE
                     LLSRLLGRPVRLISKRPDGATVERPDPEKLLELGLDAAADEVGGRILEIAQATPGDSF
                     TDDAPLHAITTATLEHIGVEALRYRPNLVIATPAGYPPYAENDWIGEEFVIGEARLRV
                     LAATTRCVVPTLEHGPLPRAPQALRIPAAENRWNASGSGAQPCAGAYFGVLVAGVIRV
                     GDRVTVEL"
     gene            complement(8227..8736)
                     /locus_tag="VFI55_00720"
     CDS             complement(8227..8736)
                     /locus_tag="VFI55_00720"
                     /inference="COORDINATES: protein motif:HMM:NF017148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HET7072591.1"
                     /translation="MAASRESYCASLPRRAPPSKRASAVEVGRSELHEFHYFYYFASP
                     DAPILTAPGTPTTKEGHNMLPPRWLAKLIIPVLVGAALTSSTAIAMADSTDDTYLTKL
                     HGLGFSWNSGSDSDMIAIGHQICADRSAGKTPDAIAADIHSSLSSKGYSFADVTGMVS
                     AAESTYCPD"
     gene            complement(8681..9088)
                     /locus_tag="VFI55_00725"
     CDS             complement(8681..9088)
                     /locus_tag="VFI55_00725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016882844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET7072592.1"
                     /translation="MSDDKALRLCDEKFVSLTTFKRNGDAIASPMWIVADGGQLVVWT
                     PSDAAKVKRVRRNPRVTLTTCGRTGKVKAGQPTVDGAAEVITDPASVDRAESLIKRKY
                     GVEFRIVTFIETVAARGRKPRVVLRITASTSAA"
     gene            complement(9419..10807)
                     /locus_tag="VFI55_00730"
     CDS             complement(9419..10807)
                     /locus_tag="VFI55_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPE domain-containing protein"
                     /protein_id="HET7072593.1"
                     /translation="MDFAALPPEINSGRMYVGPGSGPMLAAAAAWDETAAALQSAATS
                     YESEIAALSGGPWLGPASASMAAAAAPYVAWTRATAAQAELTADQARAAAVAYETAFA
                     ETVPPPVIAANRTLLMALIATNLFGQNTPAIATTETQYAEMWAQDATAMYGYASASAS
                     ATTITPFNSPGQNTNSGGAAGQAAAVAEAINTSAGNVQGAVSSAAQAVAAVPGALTGL
                     ATPAASIFGLAPLDALNLVAAVNGIFDPYLGAFGIAVDTAFGSSSLPYDVGGALTGFH
                     TDDIVSGWAGVEGWPGEGPATPLPFPVISGALEESGGLPAPMSAAMGEANTVGALSVP
                     SGWAAAAPEIRRLAMALPATSVGAALEASTAASTGSMGSVFSQMALASMAGRAMAGTG
                     AANGPLRRERIGAAKPEPTTEPQAPTEPQTPAGGPITSIAAELRELASLRDAGILTGE
                     EFDEQKKRLLAH"
     gene            complement(11126..12547)
                     /locus_tag="VFI55_00735"
     CDS             complement(11126..12547)
                     /locus_tag="VFI55_00735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7072594.1"
                     /translation="MSSTADVVVIGAGPAGVMAAAHAAELGARTVLVTQQSIGGMAAN
                     DGPVPVRTLAHTARLMRSARQLGQYGVVINEPALDYQQLLERVSEVTSDIRERSTLRT
                     LTESHGGVIQESTGPVHFVDAHTIANDTGLTLESEKFIICTGGVSKRLPIPGFEHTST
                     HSDAWNLTTVPASMIVVGCGATGAQVASVFNTFGTRVELFEASPHILPTEDEDTAAAV
                     AAAFQHEGIKLRENFGGIDSFEKTPAGVRMTFSKDGNRETAEATLAVVAVGWGADTDA
                     LKPSAAGVELTQRRFLQVNEYLQTSAPHIFAAGDVTGRVMLASEAIRDGYVAATNAVQ
                     GTRLPLTDHLIPAGSFTDPEYASVGLTERRARETHNVITAVSSYRASVRAIIDGQSSG
                     FCKLIVDEATAEILGCHVVGEQATNVVQVASIALAAGMRRVDDLARAPVSFPTYAEIL
                     IATAVRAAKKLNLEIGWRTHYTD"
     gene            complement(12667..14034)
                     /locus_tag="VFI55_00740"
     CDS             complement(12667..14034)
                     /locus_tag="VFI55_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPE domain-containing protein"
                     /protein_id="HET7072595.1"
                     /translation="MDFAALPPEINSGRMYVGPGSGPMLAAAAAWDQTAGSLYSAATS
                     YQAEVAALTAGPWLGPASASMAAAAAHYVEWTRATAALAELTANQARAAAVAYETAFA
                     ETVPPPVIAANRSLLMALIATNIFGQNTPAIATTETQYAEMWAQDAAAMYGYAGASAS
                     ATTIAPFNPPPQSTNSVGTAGQAAAVAQATDTAAGQAQSAVSSAAQAFSAVPGALTGL
                     ATPAQGIFGLAPLDVVTLLADVNGIFNPYLGTFAIGVNSGFGSSDLPFAVGGALTGLH
                     SDDIVSGWAGVEGWPGEGPATPLPFPVISGALGESEAAGGLGESVSAGLGEANTVGAL
                     SVPSGWAAAAPEIRRLAIALPATSVGAAAEASTGSAGSLFSQMALASMVGRATAGTGG
                     AGGPDRREQTGATKREPTAEPQARAGGPITSIAAELRELASRRDAGILTNEEFTRRKK
                     RLLAH"
     gene            14312..15319
                     /locus_tag="VFI55_00745"
     CDS             14312..15319
                     /locus_tag="VFI55_00745"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7072596.1"
                     /translation="MLRDAMVSRLYRRSMAEGRITVPAVPGLLDEYVQLCGNICASLG
                     VAYTPEQSVYLRSVLEAELAKAFKASQRSDIVISFQVPFGTNLNYRVKAEWRTVGDDY
                     DHWVAVRPPPLFGTEPDARVVVLAGEAADPATHRVLDVGAGTGRNALALARLGHPVDA
                     VEIAPKLADIIRSEAESESLGVHVIQSDVFTAVEGVHDEYQLMVLSEVVPDFRTTHEL
                     RGALELAADCLAPGGCLVFNTFLACPGYTPDDAAVQLGQQCNTMIFTRDELTNAVAGL
                     PFELVSDDSAYEYEKAHLPEGAWPPTGWFDGWASGLDVFDVEREDSPVELRWLVYRKL
                     G"
     gene            15375..15953
                     /locus_tag="VFI55_00750"
     CDS             15375..15953
                     /locus_tag="VFI55_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007471758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tat pathway signal sequence"
                     /protein_id="HET7072597.1"
                     /translation="MESNRRSLLMAAPLLLSALAAGDKTVKTDRPGKIGRPGDVADLP
                     LSPSPPASGPDPAETFVLPYDHMAFEAWGNLPPGSGEMAMLYGDFNQPGPYLVMMKWH
                     PGWFSAPHTYATDRIQVVVSGTWFVNSGNDFQPQDAVPVGPGGYVKRTARTPHYDGVP
                     ANRPDPAVIAIFGLGPVDIQLVDPAQPSWRQV"
     gene            16188..16592
                     /locus_tag="VFI55_00755"
     CDS             16188..16592
                     /locus_tag="VFI55_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET7072598.1"
                     /translation="MADLRVTRPRVAVLEAVRANPHADTETIFSAVRVGLPDVSRQAV
                     YDVLNALTAVGLVRRIQPLGLVARYESRVGDNHHHVVCRSCGIIADVDCAVGEAPCLT
                     PVDHHNALDGFVLDEAEVIYWGLCPDCSPEAS"
     gene            16611..18830
                     /gene="katG"
                     /locus_tag="VFI55_00760"
     CDS             16611..18830
                     /gene="katG"
                     /locus_tag="VFI55_00760"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019047195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HET7072599.1"
                     /translation="MTERNAVSSDTSQSESENPVIAAPEPKAHAPLTNQDWWPDQIDV
                     SKLHPHVAQSNPLGEDFDYATEFAKLDVDALKADMVALMTSSQDWWPADYGHYGGLFI
                     RLSWHAAGTYRIHDGRGGGGQGLQRFAPLNSWPDNATLDKARRLLWPIKKKYGNKISW
                     ADLIIFAGNVALDSMGFKTFGFAFGREDVWEPEEVLFGEEDTWLGTDRRYSGKRDLAE
                     PYGATTMGLIYVNPEGPEGQPDPIAAAHDIRATFGRMAMNDEETAALIVGGHSFGKTH
                     GAGNGDLVGPEPEAAPIEQQGFGWKSSYASGKGKDAITSGLEVVWTSTPTKWGNGFLQ
                     NLYGYEWELTKSPAGAWQFTAKDGAGAGTIPDPFGGPSRAPTMLVTDISLREDPIYRD
                     ITRRWLDYPEQLAEAFAKAWYKLLHRDMGPISRYLGPWVAAPQLWQDPVPAVDHPLVD
                     KRDIAALKNQVLESGLSIPQLVKTAWSSASSYRNTDKRGGANGARLRLEPQRNWEANE
                     PSELDKVLPVLQRIQQEFNNSATGGKKISFADLIVLAGSAAVEKAATDAGYEISVHFA
                     PGRTDASQDKTDENSFAVLEPRADGFRNYIRPGEKAPVEQLLLEKAYLLGVTAPELTV
                     LVGGLRALGANHGGSKHGVFTEQPGALTNDFFVNLLDMGTEWKPSETAENVYEGFDRS
                     SGQLKWTATANDLVFGSNSVLRALVEVYAQDDAQGKFVEDFVAAWVKVMNNDRFDLK"
BASE COUNT         3111 a         6246 c         6349 g         3225 t
ORIGIN      
        1 ctctgattcg gtctgcggct tgaacgtccc agcggcacac tgaaagcatg gtcgagttga
       61 gtgatttcga tctttgcggc gggtccgcca tttctacgtc acgaagatcg gaattccgcc
      121 atgttgggtg agatgtctac agcccaggtt gggcgcggac cacggacgct ttctagcgct
      181 cgagtccaga gacggccgtg gttggcgcag gctgttgccg tggcggcgca gtgcccgctg
      241 atttgggcgc ctcatcgcga gccagcgcga cggtcgcgac gatcatcgcc atgaccgccg
      301 ccgccaggcc cagcgccgcg gggccccgca ccgtcagata ttcaccgagc acgacaaccc
      361 cgagtagcac cgcgaccatc ggctccccga ccaacatgat cggtaccgac gcctgcaacg
      421 agcccgcatg aaacgccgac tgctgcacca cggtcaggat ggccgccagc gccaccaata
      481 cgtaaggggc ggggacggtc agcagcccat gccatccgcc caccgcgaag cggtgtgtgc
      541 agatcttggt caggacggcg atcatcccca gcagcaccgc gaccgcgacc gcgagcagca
      601 tggcacgcac gcggccagag gcgcggcggg cggcgacgac gcagacggtc accagcggca
      661 cgatgaccac caacgccaga gtccacgcct ggaccggggg ccggttatgt ccctcgtgcg
      721 gccggccgac gatcacgaag acggacagtg cggccgtcaa caccagcgcc cagccccagt
      781 cggcccggct gatgtgttgg tggcacaccc gcgcgctcag cggcaacgcg aacagcagcg
      841 acgacaccag cagcggctgg accaacagca acgagccgtg cgccaacgcc acagcttgaa
      901 acacgtaacc ggcgacagcg gccaacgtcc ctgcccacca cagcggttcg cgaagcaagc
      961 tgggcagcag cttttccgac atggcctggt cggccggcat gtcttgggcg acccgttgcc
     1021 gggtgacgat gcccaccgcg gcccagaacg cggccagcaa cgccgagaag atcgcgaccg
     1081 cgtgatgcgt gaaccactgc atgcttggca aggtacctgg cggtcaagcg cgagagaacc
     1141 cggcaccagc gcggcggaag aattcaccga cccgatacct gtggctgccc agctcaactc
     1201 cgggtggtgc gcccgacaac cgcattcagt cagctgcggg gaatgaccgg tcggggaagc
     1261 acttcagatc agtagaaggt ggtcgtgttc gcgccgtagt cgatgtacgc cgcgtgttgt
     1321 tcgaagggca ggttcccggt gttcatcaac ccactcgaga taagcagcgg ggagtaggtg
     1381 gtgcccaagc cgtactggta cagcagctga ccggtggggg cgtagaccga aatgtttgtt
     1441 cccacgggcg cgttcaggtt cgtcgggagg gttccgtcca ctccaccgga gtcgacgatc
     1501 gaggaaacgg gtattttggt agcaccgcca tcgagactaa cgtccaaggt ggtgatcggc
     1561 gatcccgtca gcgtggctat gggcggtagg ccgacactcg attggctttc gaacaccagc
     1621 tgaccagcgg gttgattgat gagcacaccc tggttgtacg gtgccggcaa tgcctgggtc
     1681 gcgatgctgg gccccggacc tcccgcgttc ggtccgacac ccagcacgcc ggtcgcgccg
     1741 tcggaggaga agaaggactg gaacgtgaat ccgttggcga tcgcggagtg gatggacacc
     1801 ggccacgcga agaattcgat gtcaacgggg gtcggcgagg tggcgactcc gccgccgaaa
     1861 ttcaccggcg cgttatacgt gaggtacaga tagtccaatc ccccgctgta gccgccgata
     1921 ccaatcccgc gggggatgcc gagttggagt actcccaata gtcccccggc gttttggaag
     1981 gggatgacca ggccggtgga tccggtgtcc accagcaggg gctggggggt tccggtgcca
     2041 acggagccgt tcacgatcgg ctcggtgccg ccgaccactg tcagcggcac ggttgcctgg
     2101 gtgccgttcg ccgagccgcc gagggcgtac gacgtcccgc cgatcagtgg acgcccgatc
     2161 aaaatctcgg tgggtgcgtt gatcgccttg atggcataga gctcgagggt cccgagcggg
     2221 tccgcgaggg ctccggcaga cgctgcctca ttcagtgtgc tctgcgccgc gccgatcggg
     2281 ctggcggccg cggcgttcag caccgagccc accgggctgc cggcggccgc cgcgactgcc
     2341 tggcccggta ggcctttgat cgccgccgcc caggtgggta gttgtgccgc cgcggcggag
     2401 acgccgccgt ggtagccgac catcgcggac acatcttggg cccacatctg ctcgtactcg
     2461 gactccgcgg cggcgatcgc gggcgcgttc tgcccgaaca ggttcgaaac gaccagctgc
     2521 acgagctggt ttcggttgac cgacaccagc gccggatgca ccgtggcggc cagcgcggat
     2581 tcgaactcac tggctaccgc ctgggcttgc gcggcggccg tttgtgcttg agctgctgcc
     2641 acgctcagca cccccgagta ctgcgcggcg gtggacagca tcgcggttga cgcggctccc
     2701 tgccaggcct cctcggtcag gcccgaggtc accgatgaga acgactgcgc cgccgaactc
     2761 aattcagcgg ccagcccctc ccacgccgca gaggcagcca gcatcggacc cgagcccgca
     2821 cccgcataca gcaacgatga aatgacctct ggtggcgaca ccaaaaaact catccctcga
     2881 gtgtccttcc ctcaacgcca agttccccca cgcctcagcc gtgacggcac ctcacgtcac
     2941 agcgacgatc ggggcacgtc gctgggccgg tagccaaact ctaggaaacc gcggctgaga
     3001 atttcgcggt ttcgccaaac gccttccctg tcaacgcccg cacaccggcg aacatgagct
     3061 cgcctcaggc atcggtcacc gtcgtccccg tggggcgcat cagaggcacc gtacggcaaa
     3121 ctttcggaag gcgcgctgca agaaaccggg aattcatcga accgactcgc gcgagccgct
     3181 ggactacctt ggcggctatg accgatcggc aagagcgcgc gatgtccttc gggtcgatcg
     3241 ccgagaacta tgacgggctg cgcccgcacg ccccgcagca ggcggtggac tggctggtgc
     3301 cgtcgggatg cgacgtggcc gtcgacgtgg gtgcgggcac cgggctgttc acccgcgcgc
     3361 tggccgggaa agcggcgcac gtcatcgccg tcgagcccga tgcgcggatg cgcgcggtgc
     3421 tgtcggcgcg atcgtcaggg gtccgggtgg tcgagggcca cggcgagtcg attccgctgc
     3481 cggacgcgtc cgccgacgcg ctgttcgtct cgtcggcgtg gcactggatg gatcccgaac
     3541 gggccgttcc cgagatcggc cgggtgttgc gcgacggcgg caggtttggc ctgatctgga
     3601 cgagccgcga ccgcgaggtg gactgggtgg ttgggctgga ccggctgccc ggcgaggagg
     3661 cgtccgaggc cgaacgattc cggcggcacc tggacgtcgt tctccccgag ccgcaaatct
     3721 tcggcgacat tgcacgcgag acgttccgat tcgtgcggac gatgagcgtc gaggacgtca
     3781 tcgcgatgct gggcacctac agcagggtca tcgtcgcgtc accgcaggac cgtgaccgca
     3841 ggctggccca agcgcgcgcc ctcctcaagg agcgtttccc atcggccgac accatcgagg
     3901 tccccatgcg ggcgcggtgt tggcgtgccg atcggatcgc ccgcggcgac ggccaatagg
     3961 ggtcttgccc ggccgccggc gcggccgtcg tcggccgtgt gtcaggattt ggcacatgag
     4021 tgcacttagc attgctcgca tcgtcggggt ttcgggtatg gcggcgtggg cgctgtggag
     4081 cgcgcccgtc cacactccgg tcgcctcggc ggatccctgc accgatgtcg cggtggtcta
     4141 cgcgcgcggc acccatcagg cccccggact cgccgatgtc ggccaggcgt tcgtcgactc
     4201 gctgacctca caggtgggtg ggcggtccct cagcgcctac gcggtcaact acccggcgac
     4261 cgacgattac cacgggagcg cgccggccgg tgccgacgat gccagcgccc acatccagca
     4321 gaccgtcgcc gaatgcccga acagcaagat cgtgctgggc ggatattcgc aggggtcgac
     4381 ggtgatcgat ctggccacca cgtcgatgcc ggccccggtg gccgaccacg tcgccgccgt
     4441 cgcccttttc ggcgagccat ccagcggatt ctcgaccgcg ctgtggggcg gccagccgct
     4501 gccgacaatc agcccgctgt acgcgggcaa gacgctcagc ttgtgcgctc ccggtgatcc
     4561 catctgctcc gcgggcggcg acatcatggc gcacgtttcg tatatccagt ccgggatgac
     4621 aacccaggcc gcgacattcg cggcgaacaa gatcggtcca gggactccga acgcgtagtg
     4681 cacccagccg gtgcacgtac cggtccgcgg cccgcgggcg ctgctgaggt cgggttaggt
     4741 cggggttaga tcgggttaga tcaggtttgt gaaatccctc ggtgcgttgt cgttcgaaac
     4801 ggtcgcggtt cggatcgtgg ttgccgtggt gatcggcgtc gcggtcgcca tcgtggtggg
     4861 caacacggcc ggttggcggt tcgcccttgt cggctgggtc gtcaccgccg ggctgtacgt
     4921 ggtgtggacg cgactcctcc tgggcggatt ggacgctgac gaaacccgcg aatatgtgac
     4981 ccgggaggac ccgacccggt gggtggccga cgcggtcgtc gtgtccgcga gtctcgcgag
     5041 cctgggcggg gtcggctatg tggtcgcggc ggggtcgcat tccggcgccg gagccatcga
     5101 agccgccatc ctcggcatcc tgaccgtcgc ggcgtcctgg tttgccgtgc acacgctgtt
     5161 ctcggtgcac tacgcgcggc tctattactc cgacgaaccg ggcggaatca acttccacga
     5221 cccggagccg ccccgcttcc gggacttcgc gtacatggcc ttcaccgtcg ggatgaccta
     5281 ccaggtgtca gacaccgaga tcgggctcac gtccatcagg gccacggtgt tgcgccatgc
     5341 gatgctgtcc tacctgctgg gcgcggtcgt cctggcggtc acgatcaacc tgatcgccgg
     5401 gctgggctcc aagctttagc gccgggtggc cgggccgctg agtgcagcta gtgtggctgg
     5461 catggccatc gagtccacca tgcttgccct cggcaccccc gctccgtcgt tctcgctgcc
     5521 cgagccggcc accggtgcca ccgtcagtct cgacgagctc acgggtccgg ccctcgtcgt
     5581 caccttcatc tgcaaccact gcccgtacgt caagcacgtc gccgccgggc tcgccgcgct
     5641 gggccgggac ctcggcgagc tgggagtcgc gatggtcggc atctccagca acgacgtcgt
     5701 gacctacccg caggacgggc ccgacgaaat ggtcgccgag gctcgccacc acggctggac
     5761 ctttccctac ctctacgacg agacccaaga cgtcgcgcgc gcctactccg cggcctgcac
     5821 gcccgacacc ttcgtgttcg acggcgagcg gcgactcgtc taccgcggcc aactggacga
     5881 ttcccgaccc aagaacggcc taccggtgac ggccgctgac gtgcgggcgg cggtcgacgc
     5941 cgtgctggcc gggcggccgg tcgacccgaa ccagcggccg tcgatcggat gcggcatcaa
     6001 gtggcgttga gccgggcgca cggcgccggc atgagctagg ccccgggcgt tcacgcgatc
     6061 atcctgggtt aggttgtgcc ggtgaaagtt gcgctggccg gctatggaag tcgcggcgac
     6121 gtcgagcctc tggccgccgt cggtcgggag ttgttgcgtc gcggtcacca cgtggcgatg
     6181 gccgccccgc cggaccagct cggcctcgtg gcgtcggcgg ggctcacctc ggtcgaatat
     6241 gggccgggca cacaggccct gcgcggccag gagttcgctc ccgacacgtc tccgaacccg
     6301 atcaacacga tggtcaagat tgcccagcat ttcacccagg ccatgacgga cttcggcacg
     6361 acgctcacgg agcaggcaga cggtgccgac ctgctgttga cgaacgcgag cgaggagggt
     6421 ctggccacca gcgtcgcgga gtattacggc attccgctgg caacgctgca cttcttcccg
     6481 ggggcccagc cgcgcggctt gatcggggac atcgcgaagg acgccgaaaa tgccctacgc
     6541 cgtcagttgg gcctgccgga gccaaccgga cgctcggccg agctggagat ccaggcgtac
     6601 gacgagttct ggtttcccgg gctggcggcc aaatgggggg aacagggccg gcgacggcca
     6661 ttcgtcggca cgctgacgct ggaattgccg acggatgtcg acgccgaggt cttgtcgtgg
     6721 attgcccagg ggacgccgcc gatctacttc ggcttcggca gcaacgtgca aatcccgtac
     6781 cccgatgaga cgatcggcgt catcagcgcg gcttgcgcgc acctgggcga gcgggcgttg
     6841 atttgctctg ggtcaagtga tttcagctgg gtcacgcatg ccgaccatgt caagatcgtg
     6901 acatcggtga accacggggc cgtctttccg gcctgccgcg cggtcgtgca tcaggggggc
     6961 gccggcacca ccgccgcagg cattcgagcc ggcatgccct cgttgattct ctggttctcg
     7021 tccgaagatc agccgacctg ggcgcaggcg atcacacgtc tgaaggtcgg ctgtgggcgc
     7081 caattctcgg ccagcaccct ggcaacgttg gtcgcggatc tgcattccgt cctcgccccg
     7141 gaatgtgccg cgcgggccca agagttggcg ccccagatga ccaaatccgc cgagagcgtc
     7201 gccaaagccg ccgacctggt ggaagaaacc gcccggctcg gccgagcgga ctgattggcg
     7261 aagacgcttc gcgtcgccta caactcgacg gtgacgcgat caccgacgcg gatgacgccg
     7321 gccaccagca cgccgaagta cgcccccgcg cacggctgcg ctccggaacc gcttgcgttc
     7381 caacggttct cggccgcggg tatgcgaagc gcctgcgggg cgcgtggcag cggtccgtgc
     7441 tcgagggtcg gcaccacgca ccgcgtggtg gccgccagga cgcgcaggcg tgcctcgccg
     7501 atgacgaatt cctcaccgat ccagtcgttc tcggcatagg gcgggtagcc ggccggcgtc
     7561 gcgatcacca ggttcggccg gtagcgcagc gcctcgacgc cgatgtgctc gagggtggcc
     7621 gtggtgatcg cgtgcaatgg ggcgtcgtcg gtgaacgagt cgcccggggt ggcctgagcg
     7681 atctccagaa tgcggccacc gacctcgtct gcagcggcgt ccagcccgag ctcgagcagt
     7741 ttttccgggt ccgggcgttc caccgtcgcg ccgtcgggcc gtttgctgat caatcggacc
     7801 ggccgtccca gcagccgcga gagcaattcg tcgacaccag ggtcgtcggc cgtgacgttg
     7861 gtcccgtcgg gtagcgcgat ccttacccgt ccggtgttcc cgtcggcggt acatttcagc
     7921 agcccgcgcc atagccgggc ctgcttggcg ctggccacgt ggccggtctg cgcatcgaga
     7981 agcgccagcc gccggtcccc ttcggcgccg tgctcgtcga cgaacatgct gtccaccgtt
     8041 tcgccgagca tcgacttcac cggatagcgg cacaaactct gcacgcgcag ctgcgcctca
     8101 cccagacccg ctgaagccgt cggcttttcc accacgcccc cgaccgagag acaccgccta
     8161 caccgtcagg gaaaactgta ggcgattacc ggccggtggc tgtcgggacg gcggccccgc
     8221 gatcgcctaa tccgggcagt aggttgattc cgcggcgctg accatgcctg tgacgtcggc
     8281 gaacgagtag cccttcgaac tcaagctgga gtggatgtcg gcggcaatcg cgtctggcgt
     8341 tttgccagcc gagcggtcgg cgcagatctg gtggccaatt gcgatcatgt cggagtcgga
     8401 gcccgagttc caactgaagc cgaggccgtg cagttttgtg aggtatgtgt catcggtgct
     8461 gtcggccatc gcaatcgccg tgctggaggt gagggcagcg ccaaccaaca ccggaataat
     8521 cagtttggcc agccagcgag gtggaagcat gttgtgccct tcttttgttg tcggcgtgcc
     8581 gggtgccgtc agtatagggg cgtccggaga agcgaaataa taaaaatagt gaaactcgtg
     8641 aagctccgac cggccaacct cgacggcgct ggcccgtttg ctaggcggcg ctcgtcgagg
     8701 cagtgatgcg cagtacgact cgcggcttgc ggccacgggc cgcgacggtc tcgatgaacg
     8761 tcacgatccg gaactccacg ccgtacttgc gcttgatcag cgattcggcc cggtcgacgc
     8821 tcgccgggtc ggtgatcacc tcggcggccc catcgacggt gggttgaccg gccttgacct
     8881 tccccgttcg gccgcaggtg gtcaacgtga cgcgcggatt gcgtcgcacc cgcttgacct
     8941 tggcagcgtc ggacggcgtc cataccacca gctgcccacc gtcggcaacg atccacatgg
     9001 gtgaggcgat cgcgtcgccg ttgcgcttga acgtcgtcag cgagacgaac ttctcgtcgc
     9061 acagacgcag ggccttatcg tcgctcatgg tgtcgtcctt tctgcgacgg acgccgcccg
     9121 agtgaccggc acggcaatag gccgcggatg tgcgagcggt gaattgctcg cgacgacggg
     9181 tcggagaatc gaatagcgtg caaacggtag caccatgcgc gagtgggacg gtggaatatc
     9241 atcccgccgg atcgtggcat tcatgggcac cgtctgcgac gaacgcccca cgaacgggac
     9301 gcgttcaccc gatggccgta gtgcgaagtg atcaaatacg cccggttacc agtctgcgca
     9361 gcattcatcg gatgaattcg attggccccc gaggctttaa ccgagagttt gccgtggctc
     9421 agtgagcgag caggcgcttt ttctgctcat cgaattcctc tccggtaagg attcccgcgt
     9481 cgcgcaggga ggccagttcg cgtagctcgg cggcgatgct tgtgatcggc ccgcccgcag
     9541 gcgtctgcgg ctccgtcggt gcctgtggct ccgtcgtcgg ttcgggcttg gccgcaccga
     9601 tccgctcacg gcgcaatggg ccgtttgcgg caccggtacc ggccatggca cgtccggcca
     9661 tgctcgccag ggccatctga ctgaacacgc ttcccatgct tccggtggag gctgctgtgg
     9721 aggcctctag ggcggcaccc acgctggtgg ccggcaatgc catcgcgagg cggcgtatct
     9781 cgggtgccgc cgcggcccag ccggacggca ctgacagggc cccgaccgtg ttcgcttcgc
     9841 ccattgcggc cgacatcggc gcaggtagac cacccgattc ttcgagagcg ccgctgatca
     9901 ccgggaacgg cagtggcgtg gccggccctt cgcccggcca gccttctacg cctgcccagc
     9961 cgctgacgat gtcgtcggtg tggaaaccgg tcagagcacc gccgacgtcg tagggaaggc
    10021 tcgacgaacc gaatgccgtg tcgacagcaa ttccgaacgc ccccagatac gggtcgaaaa
    10081 tgccgttgac ggccgccacc aagttcagcg catccagcgg cgccagaccg aagatgcttg
    10141 cagccggggt cgcgaggccg gtcagcgcgc caggcaccgc ggcaaccgct tgcgcggcac
    10201 tcgacacggc accctggacg tttccggcgg aggtgttgat ggcttccgct actgcggcgg
    10261 cctggccggc cgctcctccc gagttggtgt tctgccctgg agagttgaac ggcgtaatcg
    10321 tcgtcgccga cgctgacgcg ctcgcatagc cgtacatggc ggtcgcgtcc tgggcccaca
    10381 tctcggcgta ctgcgtctcg gtggtcgcaa tcgccggggt gttctgcccg aagagattcg
    10441 ttgcgatcag ggccatcagc agggtgcggt tggccgcgat gacgggcggc ggcaccgttt
    10501 ccgcgaacgc cgtctcgtag gccacggccg cggctctggc ctggtcggcg gtcaactcgg
    10561 cctgcgccgc ggtagctcgt gtccatgcga catagggagc cgccgcggcc gccatggacg
    10621 ccgacgccgg ccccagccac ggtccaccgc tcagggccgc aatctcggac tcatacgagg
    10681 tagccgccga ttgcagcgcg gcggccgttt cgtcccaggc cgccgcggcg gccagcatcg
    10741 gtcccgaccc ggggccgaca tacatccgcc cggagttgat ctccggcggc agggccgcga
    10801 agtccatggc gcctccctcc gctcggctga tgggccgcca gggcgccacg gttttgcggc
    10861 ggcgtaccgg tggttgctaa atccgagcca gcaatctaac gaactacgtc tagtagtagt
    10921 cgttcattca gtagtagtac atgtgcagcg gtcacaaatg tccgatgccg cgccgagcgg
    10981 gatgctcaat gggctacgaa gcctttgcca ccggttgcgt cagcgacccg acgtctcagc
    11041 cggtcccagc ggtttacgag gtgcttcggc caggggttat tcgggccggt taggacagca
    11101 ggctccagag gggctgccgg tgacatcagt cggtgtagtg ggtgcgccac ccgatctcga
    11161 gattgagttt ctttgcggcc ctgactgcgg tggcgatgag tatctcggcg taggtcggaa
    11221 atgagaccgg tgcgcgggcc agatcgtcga cgcggcgcat tcccgccgcg agggctatcg
    11281 aggcgacctg gacgacgttg gtggcttgtt cgccgactac gtgacagccg aggatctcgg
    11341 cggttgcctc atcgacgatc agtttgcaga atccgctgga ttgcccgtcg atgattgccc
    11401 tgacagaagc ccggtaagag gacaccgctg ttatgacgtt gtgcgtttcg cgcgccctgc
    11461 gttctgtgag gccgaccgag gcgtattcag gatcggtaaa gctgcccgcg ggtatcaagt
    11521 gatccgtgag cggcagccga gtgccttgca cggcgttggt ggcggcgacg tagccgtccc
    11581 gaatcgcttc ggaggcaagc atgacgcgtc ccgttacgtc accggccgca aagatatgag
    11641 gtgccgaggt ttgcaggtac tcgttgacct gaagaaacct acgctgggtc aactcgactc
    11701 cggcggcgct gggtttgagt gcatcagtgt cggcacccca accgacggct accacggcca
    11761 gcgtcgcctc ggcggtctcg cggttgccgt ccttgctgaa cgtcatccgt acgccggcgg
    11821 gcgttttttc aaacgagtcg atgccgccaa agttctcgcg tagcttgatg ccttcgtgct
    11881 ggaaggccgc tgccacagcg gctgcggtgt cctcgtcttc ggtgggaaga atatgtggac
    11941 tcgcttcgaa gagctcgact cgcgtaccaa aggtgttgaa aaccgaggcg acttgagcac
    12001 cggtggctcc gcagccgacg acgatcattg atgcagggac tgtcgtcagg ttccacgcgt
    12061 cgctgtgagt gctggtgtgt tcgaaacctg ggatcgggag ccttttgctc actccgcccg
    12121 tgcagatgat gaatttctct gactcgagcg tcaatccggt gtcgttggcg atggtgtgcg
    12181 catccacgaa atggaccggc cccgtgcttt cttggattac cccgccgtgc gactcggtca
    12241 gtgtcctcaa cgtggaccgc tcacgaatat cgctggtgac ttcgctgacg cgttcgagga
    12301 gttgctggta gtccagcgca ggttcgttga tgacgacgcc gtactgaccg agttgtcgcg
    12361 cactgcgcat caggcgcgcg gtatgagcca gcgtccgcac cggcaccggc ccgtcgttgg
    12421 ccgccatgcc gccaatcgac tgctgggtca ccagcacggt gcgagctccg agttcggccg
    12481 cgtgagcagc ggccatcacg ccggctggac ccgcaccaat cacaacgaca tctgcagtgg
    12541 acgacatgtt aggaaacctc ctgagtagct gattgcgtta cgccgacttt ggtgcgcccc
    12601 gtttccaggg ttggcatccg accgctgaag gcaaccacgg gatcggacga attcgattag
    12661 cctggctcag tgtgcgagca ggcgcttttt ccgcctggtg aattcttcgt tggtaaggat
    12721 tccggcgtcg cgccgggagg ccagttcgcg tagctcggcg gcgatgctgg tgatcggccc
    12781 gccggcccgc gcctgcggtt ctgccgtcgg ctcgcgtttc gtcgcaccgg tctgctcacg
    12841 gcggtctgga ccacccgcgc caccggtgcc ggccgtggca cgtccgacca tgctcgccag
    12901 ggccatctga ctgaacaggc tgccggcgct cccggtggag gcttcggcgg cggcgccgac
    12961 gctggtggcc ggcaatgcga tggcgaggcg gcgtatctcc ggtgcagccg cggcccagcc
    13021 cgacggcacc gacaacgccc cgaccgtgtt cgcctcgccc aaaccggccg acaccgattc
    13081 gcccaggcca cccgccgctt ccgattcgcc gagggcgccg ctgatcactg ggaacggcag
    13141 tggcgtcgcc ggcccttccc ccggccagcc ctctacgccc gcccagccgc tgacgatgtc
    13201 gtccgagtgt aaaccggtca gagcaccgcc gacggcaaag ggaaggtccg acgaaccaaa
    13261 tcccgagttg acaccaattg cgaatgtccc cagataggga ttgaaaatgc cgttcacgtc
    13321 cgccaacaag gtcaccacat ccagcggcgc cagaccaaag atgccttgag ccggggtcgc
    13381 gaggccagtc agcgcgccag gcaccgcgga aaacgcttgc gcggcactcg acacggcgct
    13441 ttgcgcctgt ccggcggcgg tgtccgtggc ctgtgccacc gcggcggcct ggccggccgt
    13501 cccgaccgag ttggtgctct gcggtggtgg gttgaacggc gcgatggtcg tcgccgatgc
    13561 cgatgccccg gcatagccgt acatcgcggc ggcgtcctgg gcccacatct cggcgtactg
    13621 cgtctcggtg gtcgcaatcg ccggggtgtt ctgcccgaag atgttcgtcg cgatcagggc
    13681 catcagcagg ctgcggttgg ccgcgatcac cggtggcggc accgtttccg cgaacgccgt
    13741 ctcgtaggcc acagccgcgg ctctggcctg gttagcggtc agctcggcca gcgccgcggt
    13801 ggctcgcgtc cactccacat agtgagcggc cgcggccgcc atggacgccg acgccggacc
    13861 cagccacggt cccgcggtga gcgccgcgac ctccgcctga tacgaggtag ccgccgaata
    13921 cagtgagccg gccgtttggt cccaggccgc cgcggcggcc agcatcggtc ccgagccggg
    13981 gcccacatac atccgcccgg agttgatctc cggcggcagg gccgcgaagt ccatgttgtc
    14041 tccctccgct cggctggcgg gccgccaggg acctggcgac tttgcgtcgg tcgtactggg
    14101 cggggccact aaaccgagcc ggcaatctaa ctacgtctag tagtagtcgc ttatttgcta
    14161 gtagtacatg tgccgcgatc acaaatgtcc gatgccgcgc cgagtcgcgt gctcaatcgt
    14221 cggcgtcgga ggatcccgtc acgcggccac ggcaccggtc gtgtagaagg gtctggtgag
    14281 tgaggcgccc tccagatcgt ttgatccgtc gatgctgcgc gatgcgatgg tgagccggct
    14341 gtatcgccgg tcgatggccg agggccggat cacggtgccc gccgtccccg gcttgctcga
    14401 cgagtacgtc cagttgtgcg gcaacatctg tgccagcctg ggcgtcgcgt acacgcccga
    14461 acagtccgtt tacctcaggt cggtcctgga agccgagctg gccaaggcct tcaaagcctc
    14521 ccagcgttcg gacatcgtca tttcgtttca ggttcccttc ggaacgaatc tgaactaccg
    14581 cgtcaaagcc gaatggcgga cggtcgggga cgattacgac cactgggttg ccgtccgccc
    14641 gccgcccctg ttcggcaccg aaccggacgc gcgcgtggtc gtgctcgccg gcgaggcggc
    14701 cgaccccgcg acccatcggg tgctggacgt cggcgccgga accgggcgca atgccctggc
    14761 gctggcccga ctcggccacc ccgtggacgc ggtcgagata gcgccgaagc tcgccgacat
    14821 cattcgctcc gaggcggaga gcgaatcgct gggcgtgcac gtcatccaga gcgacgtctt
    14881 cacggccgtg gagggtgtgc acgacgagta ccaattgatg gtgctttccg aggtggtgcc
    14941 ggacttccgg acgactcacg agttgcgcgg cgcgttggaa ctcgccgcgg attgcctcgc
    15001 tcccggcggc tgcctggtgt tcaacacgtt cttggcctgc ccgggctaca cacctgacga
    15061 cgccgcggtc caactcggtc agcagtgcaa cacgatgatc ttcaccaggg acgagctgac
    15121 gaatgccgtg gcggggcttc cgttcgagct cgtcagcgac gactcggcct acgagtacga
    15181 gaaggcccac ctgcccgagg gcgcctggcc gcccaccggc tggttcgacg gctgggccag
    15241 cgggctcgac gtcttcgatg tcgagcgtga ggattccccg gtcgagttgc gctggctggt
    15301 ctaccggaaa ttgggctgag tctcgcgcat agcgtcgggt ccggccgggt acttcaacgc
    15361 ttgtgcgggg caacatggaa tccaaccggc ggtcgctgtt gatggcggcg ccgttactgc
    15421 tgtcggcgct ggccgctggc gataagacgg tcaagacgga taggcccggc aagatcggca
    15481 ggccgggcga cgtcgctgac ctcccgctgt cgccgtcgcc gcccgccagc ggccccgatc
    15541 cggccgagac cttcgtcctg ccgtacgacc acatggcgtt cgaggcgtgg ggcaacctgc
    15601 cgccaggcag cggcgaaatg gccatgctgt atggggattt caaccagccc gggccctatc
    15661 tggtcatgat gaaatggcat ccgggttggt tcagcgcacc gcatacctac gccaccgacc
    15721 ggattcaggt ggtggtcagc ggcacctggt ttgtcaacag cggcaatgac tttcagcccc
    15781 aggacgcggt accggtcggg ccgggcggct atgtcaagcg gaccgcgcgt acgccgcact
    15841 acgacggcgt cccggcaaac cgtcccgacc cggcggtgat cgccatcttt gggctcggcc
    15901 ccgtcgacat acagctggtc gatccggcgc agccgtcatg gcgtcaggtg tgagccgggc
    15961 ccgcgtcagg ttggcatcgg ttcgccggcc tcgtacgccc ccgcaagggg ttatccttgg
    16021 gcggcaacgg tgttcggcga acggtgcgcg ccgctgggca agccgaaggg cgttgttcac
    16081 cacatatttg tgcgacatcc ttcggtagag tcttgactga atccagataa gtgcgacata
    16141 ttggcagggt gtcctcaacg gagacggatt acgcggaaca gctgcggatg gccgatctgc
    16201 gagtgacccg gcccagggtg gccgttctgg aggcagtgcg tgccaatccg cacgccgaca
    16261 ccgagacgat cttctccgcg gtgcgggtcg gcctgcccga tgtctcccgc caggccgtgt
    16321 acgacgtgct caacgcactg accgccgtcg gtctggtgcg gcgaatccag ccgttggggt
    16381 tggttgcgcg ctatgagtcg cgggttggcg acaaccacca tcacgtcgtg tgccgctcgt
    16441 gcggcattat tgccgacgtc gactgtgccg tcggcgaggc accatgctta acccctgttg
    16501 accaccacaa cgctctggat ggcttcgttc tcgacgaggc tgaagtcatc tactggggcc
    16561 tgtgccccga ctgctcgccc gaagcgtctt gatcgaagct cgattcactt atcaccgaaa
    16621 ggaatgctgt gtcatctgat acatcccaaa gcgagagcga aaaccccgta atcgctgcgc
    16681 ccgaacccaa ggcgcacgcc cccctgacga atcaggactg gtggcccgat cagatcgacg
    16741 tgtcgaagct gcacccgcac gtggcgcaat ccaatccgct cggcgaggac ttcgactacg
    16801 ccaccgagtt cgccaagctc gacgttgacg cactcaaggc cgacatggtc gcgctgatga
    16861 ccagctccca ggactggtgg ccggccgact acggtcacta cggtggcctg ttcatccggc
    16921 tgagctggca cgccgccggc acctaccgca ttcacgacgg ccgcggcggc ggcggccagg
    16981 gattgcagcg cttcgccccg ctgaacagct ggccggacaa cgccaccctg gacaaagcac
    17041 gccggctgct gtggccgatc aagaagaagt acggcaacaa gatctcctgg gccgacctga
    17101 tcatcttcgc cggcaacgtt gccctggact cgatgggctt caagaccttc ggcttcgcgt
    17161 tcggtcgcga ggacgtctgg gagcccgaag aggttctctt cggcgaagag gacacctggc
    17221 tgggcaccga caggcgctac tccggcaagc gcgacctcgc agaaccctac ggtgcgacca
    17281 ccatgggtct gatctatgtg aatccggaag gccccgaggg ccagccggat ccgatcgccg
    17341 cggcgcacga catccgcgcg accttcggcc gcatggcgat gaacgacgag gagaccgcgg
    17401 cactcatcgt gggtggccac agcttcggta agacccacgg cgccggcaac ggagacctgg
    17461 tcggccccga gccggaggcc gccccgatcg aacagcaggg gttcggctgg aagagctcct
    17521 atgccagcgg caagggcaag gacgccatca ccagcggcct ggaggtggtc tggacctcga
    17581 ccccgaccaa gtggggcaac ggattcctgc agaacctcta cggctacgag tgggaactca
    17641 ccaagagccc ggccggagcc tggcagttca ccgccaaaga cggcgccggc gccggcacga
    17701 tccccgatcc gttcggcggg ccgagccggg ccccgacgat gctggtcacc gacatttcgc
    17761 tgcgtgagga cccgatctac cgcgacatca cgcggcgttg gctggactac cccgaacagc
    17821 tggccgaggc tttcgccaag gcgtggtaca aactgctgca ccgcgatatg ggaccgatca
    17881 gccgctacct ggggccctgg gttgccgcgc cgcagctgtg gcaggacccg gttccggcgg
    17941 tcgaccatcc cctggtcgac aagagggaca tcgccgcgct gaagaaccag gtgctcgagt
    18001 ccggcctgtc tattccacag ctggtcaaga cggcgtggtc gtcggcgtca agctatcgca
    18061 acaccgacaa gcgcggcggc gcgaacgggg cgcggctgcg ccttgagcca cagcgcaatt
    18121 gggaggccaa cgagccgtcc gagctggaca aggtcctgcc cgttctgcag cggatccagc
    18181 aggaattcaa caactcggca accggcggca agaagatctc gttcgccgac ctgatcgtgc
    18241 tggcggggtc cgcggcggtg gagaaggcgg ccacggacgc gggctacgag atctcggtgc
    18301 atttcgcgcc cggccgtacc gacgcctcgc aggacaagac ggatgagaac tcgttcgcgg
    18361 tgctcgagcc gcgggccgac gggttccgca actacatccg ccccggcgag aaggcgccgg
    18421 tcgaacagct gctgctggag aaggcgtacc tgctgggcgt gaccgctccg gagttgacgg
    18481 tgctcgtcgg gggcctgcgc gccctcggcg ccaatcacgg cggcagcaag cacggggtgt
    18541 tcaccgaaca gcccggcgct ctgaccaacg acttcttcgt caacctgctc gacatgggta
    18601 cggagtggaa gccgtcggag accgcggaga acgtctacga gggattcgac cggtcgtcgg
    18661 gacagctgaa gtggacggcc accgccaacg atctggtgtt cgggtccaac tcggtgctgc
    18721 gtgcgctggt agaggtgtac gcgcaggacg acgctcaggg gaagttcgtc gaggacttcg
    18781 tcgcggcctg ggtcaaggtc atgaacaacg accggttcga cctcaagtag cggtcggaca
    18841 ggtcggcgac accccgtgag ccatcggctc gcggggtgtc gttagtttgc gcggcgaatc
    18901 ctgttgactc gcaaagcgga tcagcgggga a
//
LOCUS       DASSGQ010000015         9874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_912689,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000015 DASSGQ010000000
VERSION     DASSGQ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9874
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912689"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            482..847
                     /locus_tag="VFI55_00765"
     CDS             482..847
                     /locus_tag="VFI55_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HET7072600.1"
                     /translation="MNFNPDPIGTSAGTFRAHVAASTPPGDATPTERLTGFRQPSAHR
                     VIKPPPADQPARIQRTRRTVDLPAATHRALDIWQREAADRLGVARVTGQEVLAALVDQ
                     LLSDPKLSAQITRNIQARR"
     gene            complement(861..2540)
                     /gene="fadD2"
                     /locus_tag="VFI55_00770"
     CDS             complement(861..2540)
                     /gene="fadD2"
                     /locus_tag="VFI55_00770"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD2"
                     /protein_id="HET7072601.1"
                     /translation="MPNFIDLPFQAVSKVQQYLERGSAELHYVRKIIEAGGFRLEPPQ
                     NYAAMAAEIYKWGEFGMLPSLNARRNPDRAALIDEEGTFTYAELDEAAHAVANGLIAK
                     GVRGGDGVAILARNHRWFVIANYGAARTGARIILLNSEFSGPQIKEVSEREGAKVIIY
                     DDEYSDAVSKAEPPLGKLRALGVNPDAQQPSGSTDETLAELIARSSKAPAPKSSRHAS
                     IIILTSGTTGTPKGANRSTPPTLAPVGGILSHVPFKANEVTSLPAPMFHALGYLHATL
                     AMFLGSTLLLRRKFKPTLVLEDIEKHKATAMVVVPVMLSRVLDALEKTEPKPDLSSLR
                     IVFVSGSQLGAELAGRALKDLGPVIYNMYGSTEIAFATIAGPQDLEHNAATVGPVVKG
                     VRVRIVDDNGNEVAQGEVGRIFVGNAFPFEGYTGGGNKQIIDGLLSSGDVGYFDEHGL
                     LYVSGRDDEMIVSGGENVFPAEVEDLISGHPDVVEATAIGVEDKDWGHRLRAFVVKKE
                     DASIDEETIKHYVRDHLARYKVPREVIFVEELPRNPTGKILKRELREMEID"
     gene            2607..3848
                     /locus_tag="VFI55_00775"
     CDS             2607..3848
                     /locus_tag="VFI55_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HET7072602.1"
                     /translation="MTGPVVLEVAGRQVTITHPDKVVFERQTGGPYTKLDLVRYYLSV
                     ADGALRGVAGRPMILKRFVKGISQEAVFQKRAPSKRPDWVDVAELHYARGTSAAEAVI
                     HDAAGLAWVINLGCVDLNPHPVLAGDLDHPDELRVDLDPMPGVSWRRIVEVALVAREV
                     LEDYGLTAWPKTSGSRGFHIYARIAPRWEFRQVRLAAQTIAREVERRAPDAATSRWWK
                     EEREGVFVDFNQNAKDRTVASAYSVRATPDARVSTPLHWDEVADCKPEALTIDTVPGR
                     FAEMGDPWAGMDDAVGELDRLLMLAEEMGPPERAPKGSGKSADGRRSSTKPLIEIAST
                     KTKDEAMAALDTWRDRYPAAAGLLQPADVLVDGMRGPSSIWYRIRINLQHVPVDQRPP
                     QDELIADYSPWIRHEYRSGDR"
     gene            complement(3845..5236)
                     /locus_tag="VFI55_00780"
     CDS             complement(3845..5236)
                     /locus_tag="VFI55_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HET7072603.1"
                     /translation="MSITTTPVVAPAPPREQHRGRHWIDDWRPEDPVFWETTGNAIAR
                     RNLVFSIFAEHIGFSVWMLWSIVVVQMTAGPHGHPAASGWALTASQALCLVAVPSGVG
                     AFLRLPYTFAVPIFGGRNWTTISASMLLIPCLLLAWAVAHPHLPFGLLVAVAATAGFG
                     GGNFASSMANISFFYPEKDKGWALGLNAAGGNIGVAVVQKIVPPIVIAGGGVALSRAG
                     LLYVPLAVVAAVCAFLFMNNLSEAKADVKPVWQSLRHPDTWIMSLLYIGTFGSFIGYS
                     AAFPTLLKTVFGRGDIALTWAFLGAGIGSVIRPLGGKLADRIGGARITAVSFAMLAVG
                     ATAALWSVEAVNLPGFFASFMFLFVATGIGNGSTYRMISRIFKLKGELAGGDPDTMVQ
                     MRRQAAGGLGVISSVGAFGGFLVPLAYAWSKSHFGGIEPALRFYIAFFIALLAVTWYA
                     YLRKSSRMGQLGV"
     gene            5480..6064
                     /locus_tag="VFI55_00785"
     CDS             5480..6064
                     /locus_tag="VFI55_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET7072604.1"
                     /translation="MQATEPPLGLRERKKIKTRQAIRREAFRLFDANGYAATTVEHIA
                     EAAEISPSTFFRYFPSKESLLLADDLDPLVLAAFEAQPPDLSPAEAIRRAYEEAQTQL
                     SEEQLEFENTRQRLLFSIPELKAAMYDEYYRTVNVMAEVISRRIGRPANDFEVRVFAG
                     ALTGAMMAAYDSAPRTADTIYRALDFVDAGMPLG"
     gene            6121..7116
                     /locus_tag="VFI55_00790"
     CDS             6121..7116
                     /locus_tag="VFI55_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7072605.1"
                     /translation="MAPGWNRRGFLQLAGAAGMVAVATSAAGCSSRKPSPAAPGGSVT
                     INHLFGQTVIKEPPKRVVSAGYTEQDDLLAVGVVPIAVTNWFGDQPFAVWPWAQPKLG
                     AAQPVVLNLDNGIPIDQIGGLKPDLIVAINAGVDSDTYQKLSAIAPTVAQSDGDAFFE
                     PWKDQASAVGQAVFQSDQMKSLIDAVDQKFAAVAQKNPQWKGKKALLMQGTLWQGTVV
                     ATLAGWRTDFLNEMGLVIADNIKPFGSGHRAVIPRDQIKAVLDSADVVIWTTENPDDQ
                     KALLADAEVAASQATAQNRHIFTTKDQAGAIAFSSPLSYPLIADQLPPLIGKILG"
     gene            7230..7904
                     /locus_tag="VFI55_00795"
     CDS             7230..7904
                     /locus_tag="VFI55_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HET7072606.1"
                     /translation="MSVTDLNSSVSQELATDLVGNTVLDYGDQALMLQCGSTAEVLAW
                     TDALRAAALAGVIDMVPAARTVLVKLSDPRFQGVVRRTLRRMRVTADPEAPADRRADV
                     VIDVVYDGPDLAEVADHTGLTTAQVINAHSSTLWRVGFNGFAPGFAYLIEGDARLRVP
                     RRSEPRTSVPAGSVALAGEFSAIYPRQSPGGWQLIGHTDAVLWDLQRPNPALLTQGMW
                     VQFRAV"
     gene            7943..8857
                     /locus_tag="VFI55_00800"
     CDS             7943..8857
                     /locus_tag="VFI55_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase/urea amidolyase family protein"
                     /protein_id="HET7072607.1"
                     /translation="MTLEVLRTGPFAVVQDLGRPGLAHLGVGRSGAADRRAHKLANRL
                     VANPDDRATVEVTFGGFSARVRGGGVDIAVTGADTNPSVNGIKFGTNSIHHVRDGQVI
                     SLGAPRAGLRTYLGVRGGISVDPVLGSRSYDAMSAIGPAPLQTGDRLPVGEHTNEYPE
                     LDQAPVAGISRHLVDLLVVPGPRDDWLVDPDALVHTIWMASDRSDRVGMRLEGRPLTY
                     RHPDRQLPSEGATRGAIQVPPNGLPVILGPDHPITGGYPVVGVVTDEDVDKVAQVRPG
                     QYVRLHWARPRSTLGGRPQASVVDWPFS"
     gene            8894..9439
                     /locus_tag="VFI55_00805"
     CDS             8894..9439
                     /locus_tag="VFI55_00805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003899880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7072608.1"
                     /translation="MHTQVHTARLVHTSDLDSETRERVGQMVTTAFAGDFTRHDWEHA
                     LGGMHALIWRHGAIIAHAAVVQRRLLYGGDALRCGYVEAVAVREDCRGQGLVHALLDG
                     CEQVIRGAYQVGAVSSSAPARRVYTARGWLPWRGPTSVLAPTGPTRTCDDDGSVFVLP
                     VGIRLDTSADLMCDWRAGDVW"
BASE COUNT         1721 a         3289 c         3231 g         1633 t
ORIGIN      
        1 atgatatcaa ttcacgcaat taagtattta cgccgttcag tacttgcggt tctactcagc
       61 accgggtcga caccgagccg gatagccgca gttgacgcat catctactga gctactgata
      121 cgtacttccg cttctcagta cctcatgatc taccacttta cgtagtccga cattgaatcg
      181 cgaagattag gcagctgtta gggagttgtt aaacgatctg caaatacttt catcgaggac
      241 atgcgctcat gttttgcaac gataagcctt tctacagcta tctcctggaa aggtgcgaag
      301 aatgcgctgg tagacggctt ggcgcgcacc cgaccgccgt cggtcgccta ctgccgactc
      361 gctaggcgca cggcttggcg ccgcgacttc cgacagcgcg gatgagagaa tcagtatcga
      421 cagacgtata actgaagtac tgagttactg atttggcacg cacggaggga cggtcgtcgc
      481 cgtgaatttc aaccccgacc ccatcgggac cagcgccgga acgttccgcg cgcacgtggc
      541 ggccagcact ccgcccggtg acgcaacgcc taccgagcgc ctcacgggct tccggcagcc
      601 cagtgcccac cgcgtcatca agccaccgcc tgccgaccaa ccagcgcgta ttcaacgaac
      661 acgacgtaca gtcgacctgc cggccgccac ccaccgggca ctcgacatct ggcagcgcga
      721 agcggccgac cgcctcggag tcgcccgggt gaccggacaa gaagtcctcg ccgcgctcgt
      781 cgaccagctg ctgtccgacc ccaagctttc ggcccagatc acccgcaaca tccaggcccg
      841 gcgctgaccg gccgcggccg ctagtcgatc tccatttcgc ggagctcgcg cttgaggatc
      901 ttgccggtgg gattgcgcgg cagctcttcg acgaagatca cttcccgggg caccttgtag
      961 cgggccaggt ggtcgcggac gtagtgcttg atggtctcct cgtcgatgct ggcgtcctct
     1021 ttcttgacta cgaacgcccg cagccgatga ccccagtcct tgtcttccac gccgatggcg
     1081 gtggcttcca cgacgtcggg gtgcccactg atcaggtctt ccacctcggc ggggaacacg
     1141 ttctcaccgc cggaaacgat catctcgtcg tcacgaccgc tcacatacag caacccgtgc
     1201 tcgtcgaagt agccgacgtc gccggatgac aacagcccgt cgatgatctg cttgttaccg
     1261 ccgccggtgt agccctcgaa tgggaacgca ttgccgacga aaatccggcc gacctcgccc
     1321 tgagccacct cgttgccgtt gtcgtccacg atcctgaccc ggacgccctt gaccaccgga
     1381 ccgaccgtcg ccgcattgtg ttccaggtcc tggggcccgg cgatggtggc gaacgcgatc
     1441 tccgtcgagc cgtacatgtt gtagatgacg ggaccgaggt ctttgagcgc gcggccggcc
     1501 agctcggcgc ccagctgcga tcccgatacg aacacgatcc gcaagctgga caagtcgggc
     1561 ttgggctccg tcttttcgag tgcgtcgagg acgcgcgaca gcatcaccgg gacgacgacc
     1621 atcgcggttg ccttgtgctt ctcgatgtcc tccagcacca gcgtcggctt gaacttccgg
     1681 cgcagcagca gcgtcgaacc caggaacatc gccagggtgg catgtagata acccagagcg
     1741 tggaacatcg gcgccggcag cgacgtcacc tcgttcgcct tgaacggcac gtgcgacaag
     1801 atgccgccga ccggtgccaa tgtcggtggg gtgctgcggt tcgctccctt cggggtgccg
     1861 gtggtgccgc tggtcaggat gatgatcgag gcgtgcctgc tcgacttcgg ggccggcgcc
     1921 ttgctgctgc gtgcgatcag ctcggccagc gtctcgtcgg tgctgcccga cggctgctgc
     1981 gcgtcggggt tgactccgag cgctcgcagc ttgcccagcg gcggctcggc tttgctgacg
     2041 gcgtcgctgt attcgtcgtc gtagatgatc accttcgcgc cctcgcgctc cgacacctct
     2101 ttgatctgcg ggccggagaa ctcactgttg agcaggatga tccgggcgcc ggtgcgggcc
     2161 gcgccgtaat tggcgatgac gaaccagcgg tgattgcggg ccaggatggc cacaccgtcg
     2221 ccgccgcgca caccctttgc gatcaggccg ttcgccaccg cgtgcgcggc ttcgtcgagc
     2281 tcggcgtagg tgaaggtgcc ctcttcgtcg atcagcgccg cgcggtcggg attgcgcctg
     2341 gcgttgagcg acggcagcat cccgaactcg ccccacttgt agatctcggc ggccatcgcg
     2401 gcgtagttct gcggtggttc gagccggaac ccgcccgctt cgatgatctt gcgcacgtag
     2461 tgcaattcgg ccgagccgcg ttcgaggtat tgctgaactt tgctgacagc ctggaagggc
     2521 agatcaatga agttaggcat ggcttcacca tatgtgacgc ccgccgcatc gcggccacaa
     2581 agtttggccg aattaccatt gactccatga ccggtccggt ggtgctggaa gtggccgggc
     2641 ggcaggtcac catcacccat ccggacaagg tggtgttcga acgccagacg ggcggcccgt
     2701 ataccaagct cgacctggtc cgctattacc tgtccgtggc cgacggggcg ctgcgcgggg
     2761 tggcgggccg gccgatgatc ctcaagcggt tcgtcaaggg gatctcccaa gaggccgtct
     2821 tccagaagcg cgcacccagc aaacggccgg actgggttga cgtcgccgag ctgcactacg
     2881 cgcgcggcac ttccgccgcg gaagccgtca tccacgacgc cgccgggttg gcgtgggtga
     2941 tcaacctggg gtgcgtcgac ctgaatcccc acccggtgct cgccggcgac ctcgatcacc
     3001 ccgacgaact gcgggtcgac ctggatccga tgcccggggt ctcctggcgg cggatcgtcg
     3061 aggtcgcgtt ggtggcccgc gaggtgctcg aggactatgg cctgaccgcg tggcccaaga
     3121 cgtcgggctc ccggggcttt cacatctatg cccgcatcgc gccgcgctgg gagtttcgcc
     3181 aggtgcgcct ggccgcccag accattgccc gcgaggtcga gcggcgcgcg cccgacgccg
     3241 cgaccagccg ctggtggaag gaagaacgcg agggggtgtt cgtcgacttc aatcagaacg
     3301 ccaaggaccg caccgtcgcg tcggcctact ccgttcgggc gactcccgat gcccgggtgt
     3361 cgacgccgct gcattgggac gaggtcgccg actgcaaacc cgaggcgttg accatcgaca
     3421 ccgtgcccgg ccggttcgcc gagatgggcg atccgtgggc ggggatggat gacgcggtcg
     3481 gcgagcttga tcggctgctg atgctggccg aggagatggg accgccggag cgcgcgccga
     3541 agggatccgg caagagtgcc gatggccgac gcagctcaac gaagcccctg atcgagatcg
     3601 ccagcaccaa gaccaaggac gaggcgatgg ccgcgctgga cacctggcgc gaccgctatc
     3661 ccgcggcggc ggggctgctg cagccggccg acgtcctggt cgacggtatg cgcgggccga
     3721 gttcgatctg gtaccggatc cggatcaacc tgcagcacgt gcccgtcgac cagcgcccgc
     3781 cgcaggacga gctgatcgcc gactacagcc cttggatacg gcacgagtac agatccggcg
     3841 accgctaaac gcccaactgc cccatccggc tgctcttccg taggtaggcg taccaggtca
     3901 cggccagcag ggcgatgaag aacgcgatgt agaaccgcag cgcgggctcg atgccgccga
     3961 agtgcgactt tgaccacgcg tacgccagcg gcacgaggaa cccgccgaag gcgccgacag
     4021 acgagatgac gcccaggccg cccgcggcct gacggcgcat ctgcaccatg gtgtccgggt
     4081 cgccaccggc aagctcaccc ttgagcttga agatcctcga gatcatgcgg taggtcgagc
     4141 cgttgccgat gccggtcgcg acgaacaaga acatgaagct ggcgaagaac cccggcaggt
     4201 tgaccgcctc gacggaccac agcgccgcgg tggcgccgac ggcgagcatg gcgaagctga
     4261 cggcggtgat acgggcccca ccgatcctgt ccgcgagctt gccgcccagc ggacggatga
     4321 ccgatccgat gcccgcaccc aggaaagccc aggtcaatgc gatgtcaccg cgtccgaata
     4381 cggttttgag cagggtcggg aacgccgccg agtagccgat gaaggacccg aacgtgccga
     4441 tgtacagcag cgacatgatc caggtgtccg gatgccgcag tgactgccag accggtttca
     4501 cgtctgcttt ggcctcggac agattgttca tgaacagaaa cgcgcacacc gcggccacga
     4561 ccgccaacgg cacgtacaac agtccggcgc gcgatagcgc cacgccgccg cccgcgatca
     4621 cgatgggcgg aacgatcttc tgcacgaccg ccacaccgat gttgcccccg gccgcattca
     4681 ggccgagcgc ccagcccttg tccttctcgg ggtagaagaa cgagatgttg gccatcgacg
     4741 aggcgaagtt gccaccgccg aagcccgcgg tcgcggcaac ggcgaccagc agcccgaacg
     4801 ggagatgggg atgagcgacc gcccaggcca gcaacagaca tgggatcagc agcattgacg
     4861 cggagatggt tgtccagttg cggccgccga agatcgggac cgcgaaggtg tacggcaggc
     4921 gcaggaacgc gccgacaccg ctggggaccg cgaccaggca cagcgcctgg ctggcggtca
     4981 gcgcccagcc cgacgcggcg gggtgcccgt gtggacctgc ggtcatctgg acgaccacga
     5041 tgctccacag catccacacg ctgaagccga tgtgctcggc aaagatggag aaaaccaggt
     5101 tgcgacgggc gatggcgttg ccggtcgtct cccagaacac agggtcttcg ggtcgccagt
     5161 cgtcgatcca gtgccgcccg cggtgttgct cgcggggtgg cgcgggcgcg acgaccggtg
     5221 tggtggtgat ggacatcagg cggccccctg tcgacgaagc gcgtgcgtcc cagggcggtg
     5281 tgcgggcccg ggacatctcg gcgcgtgaac tgacggctcg acgttaagga cgcactatta
     5341 ccggcctggt ggtgcgcagt tacgtcggcg atacgtggag atcgcagcgc ccttcgggcc
     5401 cgtgtgacaa ctcgatctga actttgcggc gcacgcgtgc cccggcccgt cgccgcagcc
     5461 gatgcgggag aattacctca tgcaggcgac tgaaccgccc cttgggctgc gggaacgtaa
     5521 aaagatcaag acccggcagg ccatcaggcg cgaggcgttc cgccttttcg acgcgaacgg
     5581 ctacgccgcc accaccgtgg aacacatcgc cgaggccgca gagatatcgc ccagcacctt
     5641 cttccggtat ttcccatcca aggagtcgct gttgctcgcc gacgacctgg acccgctggt
     5701 gttggcggcc ttcgaggcac agccgcccga cctgtcgccc gccgaagcga tccgccgggc
     5761 ctacgaggag gcccagaccc aactttctga agagcaactg gagtttgaaa acacccggca
     5821 acggctgctg ttctcgatac ccgagctgaa ggccgcgatg tacgacgagt actaccgcac
     5881 cgtcaacgtc atggccgagg tgatcagccg ccgaatcggg cgcccggcaa atgatttcga
     5941 agtcagggta ttcgccggtg cgctgaccgg cgccatgatg gcggcctacg acagcgcgcc
     6001 gcggacggcc gacacgatct accgggcact ggactttgtg gacgccggca tgccgctggg
     6061 ttgagcggcc caaccgcgcg gcggtgcggg cgcgagcgag gaacgcgata atctcacgcg
     6121 atggcaccgg gatggaatcg acggggattc ttgcagctcg caggggctgc agggatggtc
     6181 gcggtcgcca catccgcagc cggctgctca tcgcgcaaac cgtcaccggc cgcccctggc
     6241 gggtcggtga caatcaacca tctcttcggc caaaccgtca tcaaggagcc gcccaagcgc
     6301 gtggtcagcg ccggctacac cgagcaagac gacctgctcg cggtcggcgt ggtgccgatc
     6361 gcggtgacca actggttcgg cgaccagcca ttcgcggtgt ggccgtgggc ccagcccaag
     6421 ctgggcgccg cgcagccggt ggtgttgaac ctggacaacg ggattccgat cgaccagatc
     6481 ggcggcctga aacccgatct gatcgtggcg atcaacgccg gcgtggactc cgacacctat
     6541 cagaagctgt cggcgatcgc cccgaccgtc gcgcagtcgg acggtgacgc cttcttcgag
     6601 ccgtggaagg accaggcctc cgccgtcggc caggcggtgt tccaatccga ccagatgaag
     6661 tcgttgatcg acgccgtcga ccagaagttc gccgccgtcg cccagaagaa cccgcagtgg
     6721 aagggcaaga aggccttgct tatgcagggc acactctggc agggcacggt ggtggcgacc
     6781 ctggcgggct ggcggaccga cttcctgaac gagatgggtc tggtcatcgc cgacaacatc
     6841 aaaccctttg gcagcggtca ccgcgcggtc atcccccgcg atcagatcaa agcggtgctc
     6901 gattccgctg acgtggtgat ctggacgacc gagaaccccg atgaccaaaa ggcgctactg
     6961 gccgacgccg aggtggcggc ttcccaggcg acggctcaga accgccacat cttcaccacc
     7021 aaggatcagg ccggcgcgat cgccttctcg tcgccgctga gctacccgtt gatcgccgat
     7081 caactgccac cgctgatcgg caagatcctg ggttgaacgc cctgggatat cacgcctgtt
     7141 tgtgcgatta ctaaggcacc tctggcagag ctcgaaaatc cctcgtcatc ccctcccgcg
     7201 aggcccgatt ccggggttac cgtcgagtaa tgagcgtcac ggaccttaac agcagcgtgt
     7261 cccaggagct ggcgaccgac ctggtcggca acaccgtgtt ggactatggc gatcaggcgc
     7321 tgatgctgca gtgcggcagc accgccgaag tgttggcctg gacggacgcg ttgcgcgccg
     7381 cggcgttggc cggcgtgatc gacatggtcc ccgccgcgcg cacggtgttg gtgaagctca
     7441 gcgacccccg cttccaggga gtcgtccgcc ggacgctacg ccggatgcgg gtcacggctg
     7501 atcccgaagc cccggcggac cgccgcgccg acgtggtgat cgacgtcgtc tacgacggcc
     7561 cggacctcgc cgaggtcgcc gatcacaccg ggctgaccac ggcgcaggtg atcaacgccc
     7621 acagctctac cctgtggcgg gtcggattca acgggttcgc acccggtttc gcgtacctga
     7681 tcgagggcga cgcgcgactg cgtgtgccgc gccgctccga gccgcggacc tcggtgccgg
     7741 cgggctccgt cgcccttgcc ggcgagttca gcgcgatcta tccacgtcaa tcgcccggcg
     7801 gctggcagct gatcggccac accgacgcgg tcctgtggga tctgcagcgg cccaatccgg
     7861 cactgctcac gcagggcatg tgggttcagt tccgagccgt gtgacgacga gtccgaagga
     7921 gctggccgtg gcaaatccgg aaatcaccct ggaagttctt cgcaccggac cgttcgccgt
     7981 cgtccaggac ctggggcggc ccgggctggc ccacctcggc gtcggccgat ccggagccgc
     8041 cgaccgccgc gcgcacaagc tggccaaccg gctggtcgcc aacccggacg accgcgccac
     8101 cgtcgaggtg acgttcggtg ggttctcggc ccgggtccgc ggcggcggcg tcgacatcgc
     8161 tgtgacgggc gccgatacca acccctcggt gaacggaatc aagttcggca ccaacagcat
     8221 tcaccatgtc cgcgacggcc aggtgatctc tttgggcgcc ccgcgcgccg gactgcggac
     8281 gtacctgggg gtgcgcggcg gcatcagcgt ggacccggtg ctcggctcgc gcagctatga
     8341 cgccatgtcc gcgatcggcc cggcaccgct acagaccgga gaccggctac cggtcggcga
     8401 gcacaccaac gagtaccccg aactcgacca ggccccggtg gcgggcatct cccgccatct
     8461 ggtggacctg ctggtggtgc cggggccgcg ggacgactgg ttggtcgatc ccgacgcgtt
     8521 ggtgcacacg atctggatgg cctccgaccg cagcgaccgg gtcggaatgc ggttggaggg
     8581 ccgaccgctg acctaccgcc acccggaccg gcagctgccc agcgagggcg ccacccgcgg
     8641 cgcgatccag gtaccgccca acggattgcc ggtgatcctg ggaccggatc acccgatcac
     8701 cggcggttac ccggtggttg gcgtggtcac cgacgaggac gtcgacaagg tcgcccaggt
     8761 gcggccaggc cagtacgtgc ggctgcactg ggcacgcccg cggtccacgc ttgggggccg
     8821 gccgcaggcc agcgtcgtgg actggccgtt ctcctagcac cggccggctg gacagggctg
     8881 gtagacaaag actgtgcaca cccaggtaca caccgcccgc ctggtccaca cctccgatct
     8941 cgacagcgag actcgggagc gcgtcgggca gatggtcacc accgcgttcg ccggcgattt
     9001 cacccggcac gactgggagc acgcgctggg tgggatgcat gccctgatct ggcggcatgg
     9061 cgcgatcatc gcgcacgccg cagtggtgca gcggcgactg ctgtacggcg gcgacgcgct
     9121 gcgctgcggc tacgtggaag ccgtcgcggt gcgggaggac tgccgcggcc agggcctggt
     9181 gcatgccctg ctggacggct gcgagcaggt gatccgcggc gcctaccaag tgggtgccgt
     9241 cagctcgtca gcgcctgccc gccgggtgta cacggcgcgc gggtggttgc cctggcgcgg
     9301 gcccacgtcg gtgctggcgc ccaccggtcc aactcgaact tgcgacgacg acggctccgt
     9361 gttcgtgctg ccggtcggga tcaggttgga cacctccgcg gacttgatgt gcgactggcg
     9421 cgccggggac gtttggtaaa tccttctctg ccgggctccc gcgttggctt atgtcaccga
     9481 atgtctttgc ggcagtggtg aattatagcc agctcgccgt ttcgttcgaa acctgatggg
     9541 ccacaacgat atttgcctgc agtgtcgagt gcgcatccgg tacatacccg ttcgatccca
     9601 ttcgatggga aatgcgcata gcgccaaatg tattccagct cacaccgaat cccacagccg
     9661 gacgaaatca cttggcaccc gggttgtcac cgtgccgaca tccgcgcctt cgacgtgtct
     9721 gacctgcacg tttccgcaac tcttgagctt cagcgagtta tcagataact cggattcaac
     9781 ttacagggga tatcagccaa atcgagttga cgtagttcga tcccgaatgt gagcatcgaa
     9841 acatacggca gggcatgcct cttcatttcg gggc
//
LOCUS       DASSGQ010000016         3527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_335743,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000016 DASSGQ010000000
VERSION     DASSGQ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335743"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..575)
                     /locus_tag="VFI55_00810"
     CDS             complement(156..575)
                     /locus_tag="VFI55_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7072609.1"
                     /translation="MLSLAEISDRLEIQQLLVDYSTAIDQRRFDDLDKVFTPDAYIDY
                     RALGGIDGRYPEVKEWLGQVLGTFPVYAHMLGNLSVRIDGDTASSRVICFNPMVLPGD
                     KDQVLFCGLWYDDEFVRTPEGWRMTRRVETKVFQKVM"
     gene            complement(611..1630)
                     /locus_tag="VFI55_00815"
     CDS             complement(611..1630)
                     /locus_tag="VFI55_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET7072610.1"
                     /translation="MSVVRGTSLSGYPRLVAELGGDPGELLRAAGIRPRDVGEHEVFL
                     PYRSLIVAVESAATATATPDFGRRLALRQGIEILGPLGVAARTAATVGDAFTIVETFL
                     GAYSPAISARITPSASAGQSFYAFEVLIERPPPHPQTTELSLGVSLRVLRFLLGAQYA
                     PVSVHLPHQPLTATADYLTYFGCRPCFTQPTAGFTFRTADLGRSLNQDALAHQAVVQY
                     LAGITTHQPGMTQSVRTMVRQLLPTGTASLELIAAQFDLHPRALHRRLSAEKTTFGEL
                     IDGVRRETAEHYLRDTDISLSHLTRELGYAEQSVLSRSCRRWFGCGPSTYRNTARSVP
                     SSRPG"
     gene            1732..>3527
                     /locus_tag="VFI55_00820"
     CDS             1732..>3527
                     /locus_tag="VFI55_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7072611.1"
                     /translation="MSFDTVIRTGRWFDGTGAPSAIRNIGIRDGHILAISAQPLDETG
                     CSEVIDARGKWVLPGMVDIHTHYDVEVLAGPSLSESLRHGVTTVMVGSCSLSTVHVDG
                     EDAGDLFARVEAIPREHVISAVNNHKTWNSCEEYIAALQARPLGPNVAAFIGHSDMRA
                     ATMGLDRATRKEVRPTKAEQARMEQMLTEALRAGFVGMSSQQLLFDKLDGETCRSRTL
                     PSTYAKPRELRRLKSLLRRNGRILQAGPDIQNPLNIASQAVQSLGIGRRQLKTTLLSA
                     ADIKANPLLIGVMGPAARLVNKLGGNFRWQHLPVPFEVYADGIDLVIFEEFGSGAAAM
                     HLKEQVAERNNLLRDEDYRRWFRKDYDSKYGLRVWHRDFFDAEIVACPDASVIGKSFG
                     QVGIERGGLHPVDAFLDLVIEHGTALRWHTTISNHRPDVLRKMAREPGIQMGFSDAGA
                     HLRNMAFYNFGLRLLRHVYDAQQAGTPFMTIEEAVHRLTGELADWYHIDAGHLRMGDR
                     ADLVIVDPAHLDDSLDGYAEEKVEQYGGLSRMVNRNDDTVSAVLVAGRAVFLNGQPTE
                     ALGTQRTGSFLRANTITPAATGTAAEPVATTV"
BASE COUNT          626 a         1139 c         1187 g          575 t
ORIGIN      
        1 cgtgtcacgc tgcaattcag cgaccagggc gggatgcccg gatccggttt tgcctcgcgt
       61 gtgtgaccgg cgtgcaagcg cccatcgggc gggtcgcgcg gcagacagcc gcctattgtg
      121 ccagaacgcg gtccttcagc ataaattcgc cggcctcaca tcaccttctg gaagaccttc
      181 gtttccaccc ggcgggtcat ccgccagccc tcgggcgtgc ggacgaactc gtcgtcgtac
      241 caaagaccgc agaacagcac ctggtcttta tcgccgggca gcaccatcgg gttgaagcag
      301 atcacccgcg acgatgcggt gtccccgtcg atgcgcacgg acaagttgcc gagcatgtgc
      361 gcatacaccg ggaaggttcc cagcacctgc cccagccact ccttgacctc gggatagcgg
      421 ccgtcgatgc cgcccagcgc ccggtagtcg atgtaggcgt ctggcgtgaa caccttgtcc
      481 aggtcgtcga atcgccgctg gtcaatggcg gtggagtagt ccaccagcag ttgctggatc
      541 tccaggcggt cagaaatttc ggccaggctc agcatgtcct cgattgaaca cgatccggcg
      601 tcgcgacgag ctagccgggc cggctcgacg ggacggaacg ggcggtgttg cggtaggtgc
      661 tgggaccgca gccgaaccat cgccggcacg accggctcag cacgctctgc tcggcgtagc
      721 cgagttctcg ggtcaggtgc gacaggctga tatcggtgtc gcgcaggtag tgttcggcgg
      781 tttcgcggcg cacaccgtcg atgagttcgc cgaaggtggt tttctccgcg gacagacggc
      841 ggtgcagcgc cctgggatgc agatcgaact gggcggcgat caactcgagg ctggcggtgc
      901 cggtgggcag gagttggcgg accatggtgc gcaccgactg ggtcatgccg ggttggtgtg
      961 tggtgattcc ggctaagtac tgcacgaccg cctgatgggc gagcgcgtcc tggttgagcg
     1021 agcgcccgag gtcggccgtg cggaaagtga agcccgctgt tggttgcgtg aagcacggcc
     1081 ggcagccgaa gtaggtcaga taatcggcgg tcgcggtgag cggctggtgc gggaggtgca
     1141 ccgacacggg tgcgtactgc gcgccgagta ggaaccgcaa cacccgcagt gacaccccga
     1201 gggacagttc ggtggtctgt gggtgcggcg gcgggcgctc gatcaaaacc tcgaatgcgt
     1261 agaacgactg cccggcgctg gcgctcgggg taatgcgcgc ggagatggcc gggctgtagg
     1321 cgcccaggaa ggtttcgaca atcgtgaagg cgtcgccgac ggtggccgcg gtgcgcgcgg
     1381 ccaccccgag cggtcccagg atttcgatgc cctgcctcag cgccaaccga cgcccgaaat
     1441 cgggagtcgc cgtcgccgtc gccgcactct caacggccac gatgaggctg cggtagggca
     1501 gaaagacttc gtgctcgccg acatctcggg gccggatgcc ggcggcgcgc agcagctcgc
     1561 ccgggtcgcc gcccagttcg gcgaccaggc gcggatagcc cgacagcgac gttccgcgta
     1621 ccaccgacat gtcaacaaat gtcaaataat cgtcaccaac tgtcaagact tcgacgctgc
     1681 catggccgca tcctgggagc caacgaggca acccgagaaa ggaacatccc catgagcttc
     1741 gacacggtta tccgcaccgg ccgctggttc gacggcaccg gcgccccgtc ggcgatccgc
     1801 aacatcggta tccgtgacgg tcacatcctg gccatcagcg cgcagccgct cgacgaaacc
     1861 ggctgctccg aagtgattga cgcacgaggc aagtgggtgc tgccgggcat ggtcgacatc
     1921 cacacccact acgacgtcga ggtgctggcc ggcccgtcgc tgtcggagtc gctgcgccac
     1981 ggcgtcacga cggtgatggt ggggtcgtgt tcgttgtcga ccgttcacgt cgacggcgaa
     2041 gacgccggtg acttgttcgc ccgcgtggaa gccatcccgc gcgagcacgt tatcagcgcg
     2101 gtcaacaacc acaagacgtg gaacagctgc gaggagtaca tcgccgcgct tcaggcccgg
     2161 ccattggggc cgaatgtcgc agctttcatt ggccattcgg acatgcgggc cgcgaccatg
     2221 ggcctggacc gcgccacccg aaaggaagtc cggcccacca aggccgagca ggcccggatg
     2281 gagcagatgc tcaccgaggc gctacgggca ggcttcgtcg ggatgtcctc gcagcaactg
     2341 cttttcgaca agctcgacgg cgagacctgc cgctcgcgca cgctgccctc gacgtatgca
     2401 aaaccacgcg aactgcgccg gctgaagtcg ctgctgcggc gaaacgggcg gatcttgcag
     2461 gcgggtcccg acattcagaa cccgctcaac atcgcatcac aggcggtgca gtcgctgggc
     2521 atcggccgca ggcagctgaa gaccactctg ctctcggccg ccgacatcaa ggccaatccg
     2581 ttgttgatcg gggtgatggg tcccgcggcc cggctcgtca acaagctggg cggcaacttc
     2641 cgctggcagc acctgccggt gccgttcgag gtgtacgccg acggcatcga tctggtgatc
     2701 ttcgaagagt tcgggtcggg tgcggccgcc atgcatctga aagagcaagt ggcagagcgc
     2761 aataacctgc tgcgcgacga ggactaccgt cggtggttcc gcaaggacta cgacagcaag
     2821 tacgggcttc gggtgtggca ccgcgacttc ttcgacgccg aaatcgtcgc gtgccccgat
     2881 gcgtccgtca ttggaaagtc ctttggacag gtcggtattg agcgtggcgg gctgcatccc
     2941 gtcgacgcat tcctcgacct ggtcatcgag catggcactg cgctgcgttg gcacacaacg
     3001 atttccaacc accgccccga cgtgctcagg aagatggccc gcgagccggg tatccagatg
     3061 gggttctccg atgcgggcgc gcacctgcgc aacatggcgt tctacaactt cggactgcga
     3121 ctactgcgcc acgtctacga cgcccagcag gccggtacgc cgttcatgac catcgaggag
     3181 gcggtgcacc ggcttaccgg cgagctggcc gactggtatc acatcgacgc cgggcatctg
     3241 cggatgggcg accgcgccga cctggtgatc gtcgatccgg cgcacctcga cgactcgctg
     3301 gacggctacg ccgaggagaa ggtcgagcag tacggcggac tgtcgcggat ggtcaaccgc
     3361 aacgacgaca ccgtcagcgc cgtgctcgtc gccgggcgcg cggtgttcct caacgggcag
     3421 cccaccgagg cgctcggcac gcagcgcacc ggcagcttcc tgcgcgccaa caccatcacc
     3481 cccgcggcca cggggaccgc ggccgagccg gttgccacga cggtgta
//
LOCUS       DASSGQ010000017         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1645, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000017 DASSGQ010000000
VERSION     DASSGQ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VFI55_00825"
     CDS             complement(<1..892)
                     /locus_tag="VFI55_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET7072612.1"
                     /translation="MTAGITGAGRTDPARIRNFCIIAHIDHGKSTLADRMLQFTGVVD
                     ARQMRAQYLDRMDIERERGITIKAQNVRLPWKVGDTDYVLHLIDTPGHVDFTYEVSRS
                     LAACEGAVLLVDAAQGIEAQTLANLYLAIENDLHIIPVLNKIDLPAAQPDRYAAELAH
                     VIGGDPSDVLRVSAKTGEGVRELLDIICRDVPPPTGDPDAPPRAMIFDSVYDIYRGVI
                     TYIRVVDGFIAPRERIRMMSTGATHELLEVGVISPEPKPTTGLSVGEVGYLITGVKDV
                     RQSRVGDTVTDATKPAKTPLA"
     gene            1019..>2172
                     /locus_tag="VFI55_00830"
     CDS             1019..>2172
                     /locus_tag="VFI55_00830"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HET7072613.1"
                     /translation="MTAHLPVGAAPSRVSTLELFFDLVFVFTITQVTEIIVDHPDAAG
                     LSQAAIVLFVIFWMYGGYAWLTNASEPDTAARRLVLLGAMAAFFVASLSVPDAFGANG
                     VAFGLAYLAVNVVHAIGFVVFAGLAALRPVLRLLGCNLISAGLVLAAGWVHGAADWVL
                     WLAAVAVQLATPALAQTRRNYRINVEHFTERHGLVILIVLGESLINVALAEAHAVVTF
                     RTAVGVFAGLLAAATIWWSYFVGDDDRASAALAAASPQRRAALAITGYFFAHLVMIYG
                     ILVLAAGIGLSAPDLTAPVDTAGAWLIAGGVAIFLVGSGGFRAALGYADPRTRIVGGA
                     CCLAAVPVGLVTSAAVELVVVAAIVALTLAADARIARSSGASGAGRRAGWA"
BASE COUNT          302 a          688 c          739 g          443 t
ORIGIN      
        1 cagctaacgg cgtcttcgcc ggcttggtgg cgtcggtaac agtgtctccg acgcgggact
       61 ggcgaacatc cttcacaccg gtgatcagat agccgacctc gccgacgctg agtccggtcg
      121 tcggcttcgg ctcgggcgag atgacaccga cctcgagcaa ctcgtgcgtg gctccggtcg
      181 acatcatccg gatccgttcg cgcggcgcga tgaatccgtc caccacgcgg atgtaggtga
      241 tgacgccgcg gtagatgtca tacaccgagt cgaagatcat tgcgcgcggc ggtgcatcgg
      301 ggtcccctgt gggcggtgga acgtcccggc agatgatgtc gagcaactcg cggacgccct
      361 cgccggtctt ggcgctgacc cgcagcacat cgctcgggtc gccgccgatg acatgtgcga
      421 gctcggccgc gtaacggtcc ggttgggcgg ccggcagatc gatcttgttc agcaccggaa
      481 tgatgtgcag gtcgttctcg atcgcgagat aaaggttggc gagggtctgc gcctcgatgc
      541 cctgcgcggc gtctacgaga agcacggcgc cctcacaggc cgccagggat ctggagacct
      601 cgtaggtgaa gtcgacatgg cccggggtgt cgatcagatg cagcacgtaa tcggtgtcgc
      661 cgactttcca agggagccgg acgttttggg ccttgatggt gatgccgcgc tcgcgttcga
      721 tgtccatccg gtccaggtat tgcgcgcgca tctgccgggc gtcgacgacc ccggtgaact
      781 gcaacatgcg atcggccaac gtggacttgc cgtggtcgat atgcgcgatg atgcagaagt
      841 tacggatgcg ggccggatcg gtgcggccgg ctccagttat tcctgcagtc acggatagct
      901 ttcagtcggt gaatagcgga ccgctgccat cgtcccatgt gggccgcgca gcgctcaggc
      961 gttcagcggg tcgcgaaatg gacaccacac cgcgccgatt ccattccacc atgggcaggt
     1021 gaccgcccac ctgcccgtcg gggctgctcc gtcgcgggtc tcgaccctcg agctcttttt
     1081 cgacctggtg ttcgtcttca cgatcaccca ggtgaccgag atcatcgtcg atcatcctga
     1141 cgccgcggga ttgagtcagg cggcgatcgt gctgttcgtc attttctgga tgtacggggg
     1201 gtacgcctgg ctgacgaacg ccagcgagcc cgacacagcc gcccgacggc tggtcttgct
     1261 cggggccatg gccgcatttt ttgtggcctc gctcagcgtg ccagatgcct tcggcgccaa
     1321 cggcgtcgcc tttggccttg cctatctggc cgtgaacgtc gtgcacgcca tcgggttcgt
     1381 cgtgttcgcc ggtctggcgg ctttgcggcc agtgctgcgg ctgctcgggt gcaacctgat
     1441 cagtgccgga ctcgtcctcg ccgccggatg ggtgcacggc gcggcggatt gggtgctctg
     1501 gctcgccgcg gtcgccgtcc agctcgcgac gccggcgctt gcccaaactc ggcggaatta
     1561 ccgcatcaat gtcgaacact tcaccgagcg gcacgggttg gtgattctca tcgtgctggg
     1621 tgagtcgctg ataaatgtcg ccctggccga ggcgcatgcc gtcgtgacgt tccgcacggc
     1681 ggtgggcgtt ttcgccggac ttctggcggc tgcaaccatc tggtggagct attttgtcgg
     1741 cgacgatgac cgggcgtcag cagctttggc ggccgcgtcg ccgcaacgcc gtgcggcctt
     1801 ggccattacc gggtacttct tcgctcatct ggtgatgatc tacgggattc tcgtgctggc
     1861 tgccggcatc gggttgtctg cgccggactt gaccgcgccc gttgacaccg ccggcgcgtg
     1921 gctgatcgcc ggcggcgtgg cgatattcct cgtcggatct ggtgggttcc gcgccgcgct
     1981 cggctacgcc gacccgcgga cccgaattgt gggcggagcg tgctgcctcg ccgctgtgcc
     2041 ggttggcctt gtcaccagcg ccgctgttga actggtcgtc gtcgcggcca tcgtcgcgct
     2101 gacactcgcc gctgacgctc gcatcgcccg ttcgagcggg gcaagcggtg ccggtcgccg
     2161 ggcgggttgg gc
//
LOCUS       DASSGQ010000018         8092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_852158,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000018 DASSGQ010000000
VERSION     DASSGQ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852158"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2047
                     /locus_tag="VFI55_00835"
     CDS             <1..2047
                     /locus_tag="VFI55_00835"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HET7072614.1"
                     /translation="RPWLLTAHSSADRGTKILNYLADVTVNQPHGPQLSTVYPHDKLP
                     QVDLSVPPIAGSKQRLAELGPEGFARWMRDTTTLNVTDTTFRDAHQSLLATRVRTTGL
                     LRVAPYIARTMPQLLSVECWGGATYDVALRFLKEDPWYRLAVLREAMPNVCLQMLLRG
                     RNTVGYTPYPEIVTTAFVEEATRTGIDIFRIFDALNNVDSMRPAIDAVRETGTAVAEV
                     AMSYTGDLSDPAETLYTLDYYLKLADQIVTAGAHVLAIKDMAGLLRAPAAAKLVSALK
                     SRFDLPVHVHTHDTPGGQLATYVAAWHAGADAVDGAAAPLAGTTSQPALSSIVAAAAH
                     TPYDTGLSLTAVCDLEPYWEALRKVYAPFESGLPAPTGRVYHHEIPGGQLSNLRQQAI
                     ALGLGDRFEDIEDAYAGADAILGRLVKVTPSSKVVGDLALALVGAGITAQDFAADPAR
                     YDIPDSVIGFLRGELGDPPGGWPEPLRTKALRGRGPAKAEQPLSVEDEALLAVSGAGR
                     QAALNRLLFPGPTKEFDAHRETFGDTSRLSANQFFYGLRHGEEHRVELERGVELLIGL
                     EAISDPDERGIRTVMCILNGQLRPVLVRDRSIASDVPTAEKADRTNPDHIPAPFAGVV
                     TVTIKVGDDVEAGQTVATIEAMKMEAAVSTPKAGKVARIAVSPTAQVEGGDMLMVIS"
     gene            2220..6113
                     /locus_tag="VFI55_00840"
     CDS             2220..6113
                     /locus_tag="VFI55_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pls/PosA family non-ribosomal peptide
                     synthetase"
                     /protein_id="HET7072615.1"
                     /translation="MLSPAAPRPRTLIDIIYETAARYPDAAAIDDGTVQLTYSELIGD
                     IEASVSWLAARGVGRGDRIGVRMPSGSYALYLAILATLATGAAYVPVDADDPDERAEL
                     VFGQANVVAVITEDGLLRGPGSSRGWRAAPPLVRDDAWIIFTSGSTGTPKGVAVTHRS
                     AAAFVDAEAQLFLQDNPIGPGDRVLAGLSVAFDASCEEMWLAWRHGACLVPAPRSLVR
                     SGMDLGPWLVTRDVTVVSTVPTLASLWPAEALEAVRLLIFGGEACPPELAERLAVGIE
                     GGREVWNTYGPTEATVVACAARLDGVGPISIGLPLPGWDLAVVDPDGQPVGYGEVGEL
                     VIGGVGLARYLDAGKDAEKYAPMQMLGWRRAYRSGDLVRLERDGLYYCGRGDDQVKIG
                     GRRIELGEVDSALVNLPGVSGAAAAVRRTAAGNPVLVGYLVSADPSFDIDEARGRLAE
                     HLPGALVPRLVQVAELPTRTSGKVDRDALPWPPPGGADVETANLGGTSGRLAGMWRDL
                     LGAGVVDGPEADFFALGGGSLAAAQLVAALRHQYPQVTVADVYDHPRLGSLAGFLDEL
                     EPTPQVTERVVRPTPRLTQLTQTILSLPLATLTALQWVTWLALGNNVARALHLVPWTV
                     AVNWWLIAAAFVLFVSPVGRMGIAVLFARTLLSNVRPGTYRRGGSVHLRVWFAERLAE
                     ASGAQNLAGAPWLVYYARALGATIGKGVDLHSLPPVTGLLTLGHRSAVEPEVDLSGHW
                     IDGDAFHVGEITIGNDATIGARTTLLPGAVVGKNADVAPGSGVLDKIKNGQYWKGSPA
                     VKSGRVNHPWPDERPPLRSRWVAMYGLTSLLLAGLPLVAAASGLAVITAAVQHTRRLS
                     QAVLPALLWAPVATLTAFTVYALLTLLAVRGLSIGLRAGYHPVRSRVGWQLWTTERLM
                     DAARNYLFPLYASLLTPMWLRLLGAKVGRGTEISTALLTPKFTVIEDGAFLADDTMVA
                     SYELGGGWIYAATTTVGRRAFLGNSGITQSGRRVPDDGLVAVLSATPSKAKRGSSWLG
                     SPPMRLRRRPAETDTATTYDPPLRLKAMRAVVETFRLLPVMVTATIGLAVLGALQALA
                     RIFGLWAAALCGGLVLLAAGAIAGAIAVVAKWLVVGRITAGEFPLWSSFVWRNEVADT
                     FVETVAAPWFARAASGTPVMNLWLRALGARIGRGTWCETYWLPEADLVELGEGATVNR
                     GCVVQTHLFHDRIMRMDRVVLEPGATLGPHCVALPAARLGAGSTVGPASLVVRGDEVP
                     GSTRWQGNPIRPWNVRRKKTRGKNAPERGVRDTAA"
     gene            6110..7435
                     /locus_tag="VFI55_00845"
     CDS             6110..7435
                     /locus_tag="VFI55_00845"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET7072616.1"
                     /translation="MKSPAKTPKKSPNVIDEYLPKNGNFGYQVSRYELDLDYRVSINR
                     LSGSATITAVTLTELQQLTLDLSSTLTVSKVSVNGKRADHFSCRGGKLRVRLASKLAT
                     GAALSIVVRYGGSPKPIRSLWGDVGFEELRDGVLVAGQPNGAASWFPCNDHPSAKAAF
                     HIQVSTDSRYRVVANGKLLSRHTRSSQTVWTYEQPEPTSTYLVTLQIGGYEMARLART
                     PVPMQAALPRRLRDDFDHDFARQPQMMKLFIELFGPYPLANGYTVVVTDDALEIPLEA
                     QGISIFGANHCDGERGSERLIAHELAHQWFGNSVTVRRWRDIWLHEGFACYAEWLWSE
                     HSGGPSADELARHHHGQLRAKPQDLLLADPGPRDMFDDRVYKRGALTLHTLRGRLGDE
                     KFFGLLKDWTTRYRHSTVVTDDFTGLAANYSDESLRPLWDAWLYSTEVP"
     gene            complement(7477..7863)
                     /locus_tag="VFI55_00850"
     CDS             complement(7477..7863)
                     /locus_tag="VFI55_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HET7072617.1"
                     /translation="MPDRNVLGSPLEPCGSDPLTGYYRDGCCSSGREDRGRHTICAVM
                     TAEFLEHQRSIGNDLLTPVPEYRFPGLLPGDRWCVTALNWLRAHHDGCAAPVVLASTH
                     ERTLEVVPLEALQEHKVDVPDDLANL"
BASE COUNT         1249 a         2727 c         2818 g         1298 t
ORIGIN      
        1 acggccatgg ctgctgacgg cgcattccag cgccgaccgc ggcaccaaga ttctcaatta
       61 cctggccgac gtgacggtca accagccgca cggtccgcag ctctcgacgg tgtatccgca
      121 cgacaaactg ccgcaggtcg atctctcggt gccgccgatt gcggggtcca aacagcgctt
      181 ggcggagttg ggaccggagg gattcgcgcg gtggatgcgc gataccacga ccttgaatgt
      241 caccgatacg acgttccgcg acgcgcatca gtcgttgctg gcgacccggg ttcgcaccac
      301 cgggttgctc agggtggcac cctacatcgc ccggaccatg ccacagctgc tgtcggtgga
      361 gtgctggggc ggcgcgacct acgacgtggc gctgcggttc ttgaaggaag acccttggta
      421 ccgcctggcc gtgctgcgtg aggcgatgcc caacgtctgt ttgcagatgc tgctgcgggg
      481 ccgcaacacg gtgggctaca cgccgtaccc ggagatcgtg acgacggcgt tcgtcgagga
      541 agccacgcgt accggcatcg acatcttccg gatcttcgat gcgctgaaca acgtcgactc
      601 catgcgacct gccatcgacg ccgtccggga aacgggcacg gcggtggccg aggtggcgat
      661 gtcctacacc ggcgacttga gcgatccggc ggaaaccctc tacacgcttg actactactt
      721 gaagctggcg gaccagatcg tcacggcggg cgcgcacgtt ttggcgatca aggacatggc
      781 gggactgctg cgcgccccgg ccgccgcgaa actggtgtca gcgctcaagt cgcgcttcga
      841 cttgcccgtg cacgtgcata cccacgacac cccgggcggg caactggcga cctacgtcgc
      901 ggcgtggcac gcgggggccg acgccgtcga cggggccgcc gccccgcttg cggggaccac
      961 cagccagccc gcgctgtcgt cgatcgtcgc cgccgcggcg cacaccccct acgacaccgg
     1021 cttgtcgctg accgcggtgt gcgatctgga gccgtactgg gaggccctgc gcaaggttta
     1081 cgcgcctttc gaatccgggc tgccggcccc gaccgggcgc gtgtaccacc acgagatccc
     1141 cggcggacag ttgtcgaacc tgcgccagca ggccatcgcg ctgggactcg gcgaccggtt
     1201 cgaagacatc gaagacgcct acgcgggcgc cgacgccatc ctcgggcgcc tcgtcaaggt
     1261 caccccgtcg agcaaggtgg tcggggactt ggcactggcg ttggtcggtg ccgggatcac
     1321 ggcgcaggac ttcgccgccg atccggcccg ctacgacata cccgactcgg ttatcggctt
     1381 cctgcgcggc gaactcggcg atcctcccgg cggctggccg gaaccgctgc gtaccaaggc
     1441 gttacgtgga cgcggcccgg ccaaggccga acagccactg tccgtcgaag acgaagccct
     1501 gctggccgtc tccggagccg gccgacaagc cgcgctgaac cgtcttcttt tccccgggcc
     1561 caccaaggag ttcgacgccc accgggagac attcggtgac acatcacggc tgagcgcgaa
     1621 ccagttcttc tacgggctgc gtcacggcga agaacatcgc gtcgagctgg agcgcggtgt
     1681 ggaactgctg atcggcctcg aggccatctc cgatcctgac gaacgtggga tccgcaccgt
     1741 catgtgcatc ctcaacggac agctgcgtcc cgtgctggtg cgggaccgca gcatcgccag
     1801 cgacgtgccg actgccgaga aagccgaccg caccaacccc gatcacattc ccgcgccctt
     1861 cgccggggtg gtcaccgtca ccatcaaggt cggcgacgac gtcgaggccg gtcagaccgt
     1921 cgcgacgatc gaagcgatga agatggaagc cgccgtttcc acccccaaag ccgggaaagt
     1981 cgcacggata gcggtgtccc cgaccgccca ggtcgaaggc ggcgacatgt tgatggtgat
     2041 cagctgaccg tcgcgtcccc acggcggccg ttgatcaacg cgccgggcgc gtggtcgccg
     2101 tcgcaacggc gcggtttgag tattttcgtg tagtggctac attctccggg ttctccgggc
     2161 tctccgggta cgccgggttc tccgggcctc ccggccctgc gcaagtcccg gcgcagtacc
     2221 tgctgtcgcc ggccgcaccg aggccgcgca cgctcatcga catcatctac gaaaccgcgg
     2281 cccgctaccc ggacgcggcg gcgatcgacg acggcacggt gcagctcacc tacagcgagc
     2341 tgatcggcga catcgaggcc agcgtgagct ggctcgccgc gcggggggtc ggccgcggcg
     2401 accggatcgg cgtccggatg ccgtcgggca gctacgcgct gtatttggcg atcttggcca
     2461 cgctggccac cggcgcggct tacgtgcccg tcgacgccga cgatcccgac gaacgagccg
     2521 agctggtgtt cggtcaggcc aacgttgtcg ccgtcatcac cgaagacggc ctgctccggg
     2581 gccccggctc gtcgcgcggc tggcgcgccg ccccaccgct ggtccgagac gacgcgtgga
     2641 tcatcttcac gtccgggtcg accggcactc cgaagggtgt ggccgtcacg caccgcagcg
     2701 ccgcagcgtt cgtcgacgcc gaagcgcagc tgttcctgca ggacaacccc atcgggccgg
     2761 gcgaccgggt gctggccggg ctgtcggtgg ctttcgacgc gtcgtgtgag gagatgtggc
     2821 tggcttggcg gcacggcgcc tgcctggttc ccgcgccgag gtcgctggtg cgcagcggga
     2881 tggacctcgg tccgtggctg gtcacccggg acgtgaccgt ggtgtcgacg gtgccgacgc
     2941 tcgcctcgct gtggccggcc gaggcactcg aagcggtccg gctgctgatc ttcggcggcg
     3001 aagcctgtcc ccctgagctg gccgagcggc tcgccgtggg catcgaaggc ggtcgcgaag
     3061 tgtggaacac gtacggcccc accgaggcca ccgtagtcgc gtgcgcggcc aggctcgacg
     3121 gtgtcgggcc gatcagcatc gggctcccat tgccgggctg ggatctggcc gtcgtcgacc
     3181 cggatggcca gccggtcggc tatggcgagg tcggcgagct ggtgatcggc ggcgtcgggc
     3241 tggcccgcta cctggacgcc ggcaaggacg ccgagaagta cgcgccgatg cagatgctgg
     3301 gctggcggcg cgcctaccgc agcggtgatc tggtgaggct ggaacgcgac gggctgtatt
     3361 actgcggtcg cggcgacgac caggtcaaga tcgggggccg ccgcatcgag ctgggcgagg
     3421 tcgactccgc actggtcaac ctgccggggg tcagcggcgc cgccgccgcc gttcgccgca
     3481 ccgccgccgg caacccggtg ttggtgggct atctcgtcag cgccgatccc tcgttcgaca
     3541 tcgacgaggc acgcggccgg ctcgccgagc acctgcccgg tgcgctggtg ccgcggcttg
     3601 tccaggtggc ggagttgccc acccgcacgt caggcaaggt cgaccgcgat gcgctgccgt
     3661 ggccaccccc cgggggcgcc gacgtcgaaa ccgccaacct gggcggcacg tcgggcaggc
     3721 tggcggggat gtggcgggac ctgctcggcg ccggtgtggt ggacggcccc gaggcggact
     3781 tcttcgcgct gggcggcggg tcgctggccg ccgcacaact cgtcgccgcg ctgcggcacc
     3841 aatacccgca ggtcacggtc gccgacgtct acgaccaccc ccggctcggc tcgctggccg
     3901 ggttcctcga cgagctcgag cccacgcccc aggtcaccga gcgggtggtg cgaccgaccc
     3961 cgcggctaac tcagctgacg cagacgatct tgtcgctgcc gctggcaacg ctcacggcgc
     4021 tgcagtgggt gacctggctc gcgctgggca acaacgtcgc gcgcgccctg cacctggtcc
     4081 cgtggaccgt ggcggtgaac tggtggctta tcgcggcggc gttcgtcctg ttcgtcagcc
     4141 ccgtgggccg aatgggcatc gcggtgctgt tcgcccggac gttgctgtcc aacgtgcggc
     4201 cgggcacgta ccgccgcggc gggtcggtgc acctgcgggt gtggttcgcc gagcgactgg
     4261 ccgaggccag cggcgcacag aacctggccg gggcgccgtg gctggtgtat tacgcccgtg
     4321 ccctcggcgc caccatcggc aagggcgtcg acctgcattc gttgccgccc gtcaccgggt
     4381 tgctgacact cgggcatcgc agcgccgtcg aaccagaagt ggacctgtcc gggcattgga
     4441 tcgacgggga cgccttccac gtcggcgaga tcacgatcgg caacgacgcc acgatcgggg
     4501 cccggaccac gctgctgccc ggcgcggtcg tcggcaagaa cgccgatgtg gcaccgggtt
     4561 cgggcgtcct cgacaagatc aaaaacggcc agtattggaa gggctcgccc gcggtgaagt
     4621 ccggccgggt caaccatccg tggccggacg agcggccgcc gctcagaagc cggtgggtcg
     4681 cgatgtacgg gctgacatcg ctactgctcg ccggtctgcc gttggtggcc gcggcgagcg
     4741 gcctcgcggt gatcaccgcc gccgtccagc acacccgccg cctttcgcag gcggtgcttc
     4801 cggcgctgct gtgggcaccg gtcgccacgc tgaccgcctt cacggtgtat gcgctgctga
     4861 cgctgctcgc ggtgcggggg ttgtcgatcg ggctacgtgc gggctaccac ccggtgcgca
     4921 gccgggtcgg ctggcagctc tggacgaccg agcggctcat ggacgccgcg cgtaactacc
     4981 tgttcccgtt gtatgccagc ctgctgacac cgatgtggtt gcggttgctc ggcgccaagg
     5041 tcggcagggg caccgagata tccacggcgt tgctgacccc caagttcacg gtgatcgagg
     5101 acggcgcgtt tttggccgac gacacgatgg tcgcgtccta cgagctcggc ggcggctgga
     5161 tctatgcggc caccaccacc gtgggcaggc gcgcgttcct gggcaactcg ggcatcaccc
     5221 agtccggccg ccgcgtgccc gacgacggac tggtcgcggt gctgtcggcc acgccgtcga
     5281 aggccaagcg cggctcgtcc tggttgggca gcccaccgat gcggttgcgc cgtcggcccg
     5341 cggagaccga cacggcgacc acctatgacc cgccgttgag gttgaaggcg atgcgggcgg
     5401 tggtggagac gttccggttg ctgccggtga tggtgacggc aacgatcggg ctggcggtgc
     5461 tgggcgcgtt gcaggcgctg gcaaggatat tcggcctgtg ggcggcagcg ctgtgcggtg
     5521 gcctggtgct gctggcggcg ggcgcgatcg cgggagcaat cgccgtcgtc gcgaaatggc
     5581 ttgtggtggg caggatcacg gccggcgaat ttccactgtg gtcgtcgttc gtgtggcgca
     5641 acgaggtcgc cgatacgttt gtcgagacgg tcgcggcacc gtggttcgcc cgcgcggcga
     5701 gtggcacacc ggtgatgaat ctgtggctgc gggcgctggg cgcccgcatc ggtcgcggca
     5761 cctggtgtga aacctattgg ctccccgagg cggacctcgt cgaactgggc gagggcgcga
     5821 cggtcaatcg cggctgcgtg gtgcagacgc acctgttcca cgaccggatc atgcgcatgg
     5881 accgcgtcgt gctggaaccc ggcgcgaccc tggggccgca ttgcgtggcg ctgcccgccg
     5941 cccggctggg tgctgggtcg accgtcggac ccgcgtcgct ggtggtgcgc ggcgacgaag
     6001 taccggggtc gacacgatgg cagggcaacc cgatcaggcc gtggaacgtg cgccgcaaga
     6061 agacccgggg caagaacgcg cccgagcgcg gggtgcggga cacggcagcg tgaaatcgcc
     6121 tgccaagacg cccaagaagt caccgaacgt catcgacgag tacctgccga agaacggcaa
     6181 cttcggctac caggtgtcgc ggtatgagct cgatctcgac tacagggtct cgatcaatcg
     6241 cctctccggg tcggcgacca tcaccgcggt gaccctgacc gagttgcaac agctcacgct
     6301 cgacctgtcc agcactttga cggtgtccaa ggtgtcggtc aacggcaagc gcgccgatca
     6361 tttctcgtgt cgtggaggca aactacgcgt ccggctggca tcgaaactgg cgaccggcgc
     6421 cgcgctgtcg atcgtggtgc gctacggggg ttccccgaaa ccgatccgat cgctatgggg
     6481 cgacgtcggt ttcgaggagc tgagagacgg cgtgctggtc gccgggcaac ccaacggtgc
     6541 cgcgtcgtgg ttcccctgca acgaccaccc cagcgccaag gcggccttcc acatccaggt
     6601 cagcaccgac agtcgctacc gggtggtggc caatggaaag ctgctgtcgc gccacactcg
     6661 gtcgtcgcag accgtgtgga cctatgagca acccgagccg acgtcaacct acctcgtcac
     6721 gctgcagatc ggcgggtacg agatggcccg gctggcaagg acaccggtgc cgatgcaggc
     6781 ggcgctgccc aggcgcctgc gtgacgactt cgaccacgac ttcgcccgcc aaccgcagat
     6841 gatgaagctg ttcatcgagc ttttcggacc ctacccactg gccaacgggt acacggtcgt
     6901 cgtcaccgac gatgcgctgg agataccact tgaggcccag gggatttcga ttttcggcgc
     6961 gaaccattgc gacggcgagc ggggcagcga acggctgatc gctcacgaac tggcgcatca
     7021 atggttcggc aattccgtga ccgtgcggcg gtggcgtgac atctggctgc acgagggatt
     7081 cgcctgctac gcggagtggc tgtggtcgga acacagcggc ggccccagcg ccgacgaact
     7141 ggcccgccac caccacgggc aactgcgcgc caagccgcaa gacctgttgc tggccgaccc
     7201 cggaccgcgt gacatgttcg acgaccgggt gtacaagcgc ggtgcgctga ccctgcacac
     7261 tctgcgcggc cgcctgggcg acgagaaatt ctttgggctg ctgaaggatt ggacgacacg
     7321 gtatcgccac tcgaccgttg tgaccgacga cttcaccggc ctggcagcca actactccga
     7381 tgagtcgctg cggccgctgt gggatgcgtg gttgtactcg accgaagttc cctgacttca
     7441 gctcagcccg acggatcggg gcccaagccg cgacgtctag aggttagcca ggtcgtcggg
     7501 aacgtcgact ttgtgttcct ggagcgcctc gagcggcaca acctccaagg tgcgttcgtg
     7561 ggtggacgcc aacaccacgg gtgctgcgca accgtcgtgg tgggcccgaa gccagttcag
     7621 cgcggtgacg caccagcggt cgccgggcaa aagaccgggg aacctgtact cgggaaccgg
     7681 cgtcaacaag tcgttgccga tggaacgctg gtgctccagg aattcggccg tcatcaccgc
     7741 gcagatggtg tgccggccac ggtcttctcg gccgctcgag caacacccgt cgcggtagta
     7801 accggtgagc gggtcactgc cgcatggttc cagcggactg ccgagcacgt tgcgatcagg
     7861 cacccgtcaa gtatcgcgcc ctcggggctg aagtttgaag gaacttcacc atctgcgccg
     7921 gggcgaattt tctccgtgcc gttacccggt ctcccgggtc cggccccggg ggacatatac
     7981 agtgggccga atcgatgccc cgaccccgat tcgcgcagac cccagatcgc aggagtgact
     8041 tcagcgcaat ggtgacgcct aaccaacccg gcaacccgtt cgatgagcag cc
//
LOCUS       DASSGQ010000019         6965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1277659,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000019 DASSGQ010000000
VERSION     DASSGQ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277659"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VFI55_00855"
     CDS             complement(<1..275)
                     /locus_tag="VFI55_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7072618.1"
                     /translation="MPKVSHYAQGTPDWVDLQTTDQSAAKQFYAALLGWGYDDNTMPE
                     GGVYSMATVNGETVAAIAPMPPAAPEGRPPMWNTYIAVDDVDAAVDKV"
     gene            586..1746
                     /locus_tag="VFI55_00860"
     CDS             586..1746
                     /locus_tag="VFI55_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HET7072619.1"
                     /translation="MRIGVGVSTAPGARQAAVEAAAHARDELAGEAPSLAVLLGSRPH
                     TDKAVEVLDAVQEMVEPPALIGCVAQAIVAGRKEMEDEPAVAVWLASGLAAETFQLDF
                     VRTGAGGLLTGYRFDRTAHDLHLLLPDPYTFPSSLLIEHLNTDLPGLTVVGGVVSGGL
                     RPGDTRLFRDQGVLTSGLVGMRLPGMHGIPIVSQGCRPIGDPYIVTGADGALITELGG
                     RPALQRLRAIVEGLPRHEQELVSHGLQIGIVVDERLAAPGQGDFVIRGLVGGNPSTGA
                     IEIGEVVEVGETVQFQVRDPVLADRDLRLAVERAGAQLPGRPVGALLFTCNGRGRRMF
                     GVADHDASTIDELLGGIPLAGFFAAGEIGPIAGRNALHAFTASMALFTEDTG"
     gene            complement(1792..2724)
                     /locus_tag="VFI55_00865"
     CDS             complement(1792..2724)
                     /locus_tag="VFI55_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7072620.1"
                     /translation="MPRTDNDTWNLATSVGATATMVAAARAIATKADNPLIDDPFAEP
                     LVRAVGVDFFTRWAAGEVVADDLDDHESDWKLGYMPDAMAVRTRFFDAFFRDATQAGI
                     RQAVILASGLDARAYRLAWPADMTLFEIDQPEVIEFKTITLAGLGAVPKTDLRTVAID
                     LRQDWPTALLDIGLDTSQPTAWIAEGLFGYLPPEAQDRLLDNITSLSATGSRLACEAM
                     PDRSEVDVEEAREKMRRATAKWRDHGLELDFEDLGFEGERNDVASYLQNLGWQSVAIR
                     MSELLVDNGLAAIPQNNDSVSMADTIYYSSVLSK"
     gene            complement(2815..4254)
                     /locus_tag="VFI55_00870"
     CDS             complement(2815..4254)
                     /locus_tag="VFI55_00870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HET7072621.1"
                     /translation="MKQLSGLDAAFLTLETHNSTGHVGGLLILDPSESPAPLDLAALT
                     RLMNERIPQIPLLRQRLKYVPLGLDQPYWIDDENFDIEFHVREISLPAPGSDAQLTEQ
                     VSRLHARPLDRRRPLWEMYLISGLGGGRLAVYTKIHHAAIDGVSGAELMTVLVDLTPE
                     GENRSPAPDFKPEPAPGDATLLARAAASMATRPVSAARIAVGVGRALPLVGSALSPYV
                     GALLGRGDRDGDVIKASPGLAPRTPFNDEVTLHRRFAMRSVELDDVKTVKNAFGASVN
                     DVVMAMSAGAVRHWLIDHDALPEAPLVAMVPVSVRDDASKSKLGNKVSAMFASLPTHL
                     DDPVARLAAVHEATKVAKAQQAAIPPGLVENVTDFAPPALVARAARVVFAMGLPRRIP
                     PYNLVISNVPGPNVDLYLGGAKVLANYPVSVVTDGQGFNITVNSNSGQLHFGLIGCRE
                     LVPDIDVIAGYLVEELDKLLKLAHEVEDA"
     gene            complement(4251..5210)
                     /locus_tag="VFI55_00875"
     CDS             complement(4251..5210)
                     /locus_tag="VFI55_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009477561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7072622.1"
                     /translation="MMQPQLARPWGGASHVSDIAGPVHWVDFGGPAGAPPIVMVHGLG
                     GSHLNWVRVARPLTERNRVIAIDLPGFGFSPAAGRKTSIEANTKILDRFIGEIVGGPV
                     ILMGNSMGGAITILESAASPHAVAAVVLVDPALPRPAEVPDAQVAAQFVIYALPGVGE
                     AFMDISRRSRTDRELVERVVNLCFADPRRSSPEVMDASTQMLTHRRRFMPSQDAAFLQ
                     AARSLLRAGLRPGQFKNTMRSLVQPVLLIHGDRDRLVPISAARLAATANPWWDTEFLH
                     DVGHTPQLEAPDAVIRAVQRWLDRHELAAAGAANMTAPIEENR"
     gene            complement(5221..5826)
                     /locus_tag="VFI55_00880"
     CDS             complement(5221..5826)
                     /locus_tag="VFI55_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012689265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7072623.1"
                     /translation="MSVTTACVVSSSKAAARIRAAAIEAFAEHGYGGTTTRDIAARLG
                     LSAAAMYPHYKSKEELLFVIAYEGHQKTVEWLQAADPVSESPAKRLRAVVAEFARTQA
                     AYHGRSRVVEYELTALSPDHYRVILGLRRKLTRHLREIVEAGAADGSFAVPDADGVTI
                     AIVSLCVDVCRWFPAGRYADPETVANLHSELALRLVNATTE"
     gene            complement(5924..6853)
                     /locus_tag="VFI55_00885"
     CDS             complement(5924..6853)
                     /locus_tag="VFI55_00885"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7072624.1"
                     /translation="MDRNDPLPSWRPGSTRDALEAFLAAAEEVPVVDRVAYFDNDGTL
                     WCERPSYVQLDFFLDALRKRVVEDPSIGERAEFAAVLSGDRAAVAGIGLERIALALAG
                     LFEGLTPQEFTSAAREFMARAAHPTLGRPTRSVIYQPMSELIAELRRRDFTVGIVTGG
                     GTEFVRAISDDLYGVPAELVVGTLIGYDVSRDGQGPPVIRRTSGLVGVANEGSAKVAN
                     IQTQLGRRPILAAGNSGGDREMLEWAQASRAAGSPSLALLIDHDDADREFHYTSTAQT
                     FAETEPITDVGARLGWTIVSMARDWDVVFPPVS"
BASE COUNT         1127 a         2249 c         2391 g         1198 t
ORIGIN      
        1 accttgtcga ccgccgcatc gacgtcgtcc accgcgatat aggtgttcca catcggcggc
       61 ctgccctctg gtgcggccgg cggcatcggc gcgatcgccg ccaccgtttc tccgttgacc
      121 gtggccatgg agtacacgcc gccttcaggc atcgtgttgt cgtcgtaacc ccagcccaac
      181 agcgctgcgt agaactgctt ggccgcggac tgatcggtgg tttgcaggtc gacccagtcc
      241 ggcgtgccct gagcgtagtg acttaccttg ggcatgattc accctccatt tggctaatga
      301 atcggcaccg acccgtggag caggcgagtc gatcgaggat tcggggatcg ggcccgatcg
      361 cggcggttcg gcgaaaggcc gcacacccgc gccttccggc gcgacgagct tgcggcaaac
      421 ccccagcaaa taccggacac cggctttcac gctaccaccg gccgacgggt agtcctagcg
      481 aaagcctaag tgtggaatcg aaattcgagc gagaggatgg ccgctggcgt ggctgtcccg
      541 caccgatcca acacgaggcc gtcggcatcg gcgcatcatg ggagtgtgcg gatcggagtt
      601 ggggtttcca ccgcgcccgg cgcacggcag gccgcggtgg aggccgcagc gcacgcgcgc
      661 gacgagctcg cgggtgaggc gccatcgctg gccgtgctac tgggatcgcg tccacacacc
      721 gataaggccg tcgaggtcct cgacgcggtg caagagatgg tcgagccgcc cgcgctgatc
      781 ggttgcgtcg cccaggcgat cgtcgcgggc cgcaaggaga tggaggacga gcccgcggtg
      841 gcggtgtggc tggcgtccgg cctggccgcc gagacgttcc agctggactt cgtccgcacc
      901 ggcgcgggtg gcctgctcac tggttaccgg ttcgaccgga ccgcacacga cctccacctg
      961 cttctcccgg acccgtacac atttccgtcg agcctgctca tcgagcacct caacacggac
     1021 ctgccggggc tgaccgtggt gggcggcgtc gtcagcggtg ggctaagacc gggcgacacc
     1081 cgactgttcc gcgaccaggg cgtgctgacc tccggcctgg tgggcatgcg cctgcccggc
     1141 atgcacggta tcccgatcgt gtcgcagggc tgccggccga tcggcgaccc ctacatcgtc
     1201 accggcgcgg acggagcact catcaccgag ctgggcggcc gaccggcgct gcaacgcctg
     1261 cgtgcgatcg tcgaggggtt gccgcgccac gagcaggaac tggtcagcca cggcctgcag
     1321 atcgggatcg tcgtcgacga gcgcttggcg gctccgggtc agggcgactt cgtgatccgc
     1381 gggctggtcg gcggcaaccc ctcgactggg gcgattgaga tcggcgaggt cgtcgaggtc
     1441 ggcgagaccg tgcagttcca ggttcgtgac ccggtgctgg ccgacaggga cctgcgcctg
     1501 gccgtggagc gggcgggagc gcagctaccc gggcgtccgg ttggcgcgct gttgttcacc
     1561 tgcaacgggc gtgggcggcg catgttcggg gtcgccgacc atgacgcgtc gacgatcgac
     1621 gagctgcttg gcgggattcc gctcgccggc ttcttcgccg ccggggagat cggcccgatc
     1681 gcgggccgca acgcgttgca cgcatttacc gcgtcgatgg cgctgttcac ggaagacaca
     1741 ggatgaggcg tacctgggcg cggaagatcc cgtcgggatg cggcggacgg cctatttgct
     1801 cagtaccgag ctgtagtaga tggtgtcggc catagacacc gagtcgttgt tctgtgggat
     1861 tgccgcaaga ccgttgtcga ccaacagctc actcatccgg atagcgactg actgccatcc
     1921 caggttctgc aggtaggacg ccacatcgtt gcgctcgccc tcgaagccaa ggtcttcgaa
     1981 gtccagctcg aggccgtggt cgcgccattt ggcggtagcc ctgcgcatct tctcgcgcgc
     2041 ttcttcgacg tcaacctccg acctgtccgg catcgcttcg caagccagcc ggctgcccgt
     2101 ggcgctgagc gaggtgatgt tgtcgagcaa gcggtcctga gcttctggcg gcagataccc
     2161 gaagagcccc tcagcgatcc aagcggtcgg ctggctggtg tccaggccga tgtcgagcag
     2221 ggctgtgggc cagtcctgcc gcagatcgat ggcgacggtg cgcaggtcgg tcttcgggac
     2281 ggcgcccagc ccggccaggg tgatggtctt gaattcgatc acctcaggct ggtcaatctc
     2341 gaacagcgtc atatcggcgg gccacgccag ccggtaggcg cgcgcgtcga gaccggacgc
     2401 caggatcact gcctgacgga ttccagcctg ggttgcatcg cgaaagaatg cgtcgaagaa
     2461 acgagtccgg acggccatcg catcgggcat gtagccgagc ttccagtcgg actcgtggtc
     2521 gtcgaggtca tcggcgacaa cctcgccggc ggcccagcgg gtgaagaaat ccactccgac
     2581 ggcgcggacc agcggttcgg caaacgggtc gtcgatcagg gggttgtcgg ctttggtggc
     2641 gatcgcgcgg gcggccgcca ccatcgtggc cgtggcgccc acgctggttg ccaaattcca
     2701 ggtgtcgttg tcggtgcgtg gcatgagcct gctctcgggt gtcggatagg gatatactta
     2761 gatagtttat ctaactatag caggcaccga ccgagcgtca agtccgagct tgtcctaggc
     2821 gtcttccacc tcgtgcgcga gtttcaacaa cttgtcgagt tcctcgacga ggtagcccgc
     2881 gatgacatcg atgtccggca ccagctcgcg gcagccgatc aacccgaaat gcagctgccc
     2941 gctgttgctg ttgacggtga tgttgaatcc ctggccgtcg gtgaccaccg acacggggta
     3001 gttggccaac actttcgcgc cgcccaggta aaggtcgacg ttcgggccgg gcacattcga
     3061 aatcaccagg ttgtagggcg ggatccggcg gggcaaaccc atcgcgaaca cgacccgagc
     3121 cgctcgggcc accagcgcgg gcggtgcgaa gtcggtgacg ttctcgacga gaccgggcgg
     3181 aatcgccgcc tgttgggcct tggcgacctt ggtcgcctcg tgcacggcgg caaggcgggc
     3241 aacggggtcg tcgaggtggg tcggcagcga ggcgaacatc gcggacactt tgttgccgag
     3301 cttggacttg ctggcgtcgt cgcgcaccga gacgggaacc atcgcgacca gcggagcctc
     3361 gggcagcgca tcgtgatcga tcagccagtg ccgcaccgcg ccggccgaca tcgccatcac
     3421 gacgtcgttg acagacgcgc cgaatgcgtt cttgacggtc ttcacgtcat cgagctcgac
     3481 gctgcgcatg gcgaaccgcc ggtgcagggt gacctcgtcg ttgaacgggg tgcgcggcgc
     3541 gagcccgggg gaagctttga tgacgtcgcc gtcccggtcg ccgcgaccga ggagcgcgcc
     3601 gacgtagggc gagagagcac tgccgaccag cgggagcgcg cggcccaccc ccacggcgat
     3661 ccgtgcggcg ctcaccgggc gcgttgccat cgacgcggcg gcacgcgcga gcagcgtcgc
     3721 gtcgcccggt gccggttcgg gtttgaaatc gggcgcggga gaacgattct caccctcggg
     3781 tgtcaggtcg accaggaccg tcatcagctc ggcgcccgac acgccgtcga tggcagcgtg
     3841 atggatcttc gtgtacaccg ccagccgccc gcctccgagc cccgagatca ggtacatctc
     3901 ccacagcggt cgccgccggt cgagcgggcg cgcatggagc cggctgacct gttcggtaag
     3961 ttgcgcgtcc gaacccggtg cgggaaggga gatttcacga acgtggaatt cgatgtcaaa
     4021 gttctcgtca tcgatccaat acggttggtc gagtcccagc ggcacgtact tcaaccgttg
     4081 ccgcagcagc gggatctgcg gtatgcgctc gttcatcagc ctggtcaacg ccgccagatc
     4141 caacggcgcc ggggactcgc tcggatcgag aatcagcaag ccgccgacgt gtccggtgga
     4201 gttgtgggtt tcgagcgtca agaaggccgc gtctaggccg gagagctgtt tcatcggttt
     4261 tcctcgattg gtgcggtcat gtttgcggcg ccggcggcgg caagttcgtg gcgatccagc
     4321 caacgctgca cggcgcggat caccgcgtcg ggagcctcga gctgcggggt gtgtccgacg
     4381 tcatggagaa actcggtgtc ccaccagggg ttcgccgtgg ccgcgaggcg tgcggcgctg
     4441 atgggcacca gccgatcccg atcgccgtgg atgagcagca ccggctgcac cagcgaccgc
     4501 atcgtgttct tgaactgacc cggccgcagg ccggcccgca gcaacgaccg tgcggcctgc
     4561 aggaaggcgg cgtcttggga gggcatgaag cggcggcgat gtgtgagcat ttgggtggag
     4621 gcgtccatca cctccgggga gcttcggcgc ggatcggcga agcacaggtt cacgactcgt
     4681 tcgacgagct cccggtcggt tcttgatcgt ctgctgatgt ccatgaaggc ctccccgacc
     4741 ccgggaaggg cgtagatcac gaactgtgcg gcgacctggg cgtcgggaac ctccgcgggt
     4801 cgcggcagtg ccggatcgac cagcaccacc gcagcgaccg cgtgcgggct ggcggccgac
     4861 tccaggatcg tgatcgcacc gcccatcgag ttgcccatca aaatgactgg gccgccgacg
     4921 atttcaccga tgaatcggtc gagtatcttc gtgttggctt cgatgctggt ctttctgccc
     4981 gcggcgggcg agaacccgaa tccgggcagg tcgatggcga tcactcggtt gcgttcggtc
     5041 agcggtcgag cgaccctgac ccagttcaag tgggagccac ccagtccgtg caccatgacg
     5101 attggcggag cccctgccgg cccgccgaag tccacccagt gcaccggtcc ggcgatgtcg
     5161 ctgacgtgac tggcgccgcc ccagggcctg gcgagttgcg gttgcatcat gtggtcggtc
     5221 ctattcggtg gtggcgttga cgaggcgcag cgccaattcg gagtggaggt tggcgacggt
     5281 ttcgggatcg gcgtagcgcc cggccgggaa ccagcggcag acgtcgacac acagggaaac
     5341 gatggcaatg gtgacgccgt cggcgtcggg aaccgcaaaa gatccgtcgg cggcgccggc
     5401 ttccacaatt tctcgcagat gccgcgtcag tttgcggcgc agcccgagga tcactcgata
     5461 gtggtccggc gagagtgcgg tcaactcgta ttcgaccacc cttgaccggc cgtgataagc
     5521 cgcctgcgtc cgggcgaact cggcgactac cgcccgtagg cgcttggcgg gcgattcgct
     5581 gactggatcg gcggcctgca gccattcgac agtcttttga tggccctcgt acgcaatgac
     5641 gaaaagcagt tcttctttgg atttgtagtg cgggtacatc gcggccgcgc tcaaaccgag
     5701 gcgtgctgcg atgtcacggg tggtcgtacc gccgtaaccg tgctcggcga acgcctcgat
     5761 tgcggcggcc ctgattcgcg cggcggcctt ggaactgctg acaacgcacg ccgttgtcac
     5821 gctcacccga agctccacat gctgcgacta taagcgaccg cttatttata agcaagcgct
     5881 tagtccggaa aactgtggat ggcggcgcgg aggacgaagc tgatcaagaa accggcggaa
     5941 ataccacgtc ccagtcacgg gccatgctca cgatggtcca tcccaggcgt gccccgacgt
     6001 cggtgatcgg ctcggtctcg gcgaacgtct gtgccgtact ggtgtagtgg aactcgcggt
     6061 cggcatcgtc gtggtcaatc agcagcgcga ggctgggaga gccggcggcc ctggaggctt
     6121 gagcccattc cagcatctcc cggtcacccc cggagttgcc ggcggccagg atcggccgcc
     6181 ggcccagctg ggtctggatg ttggcgacct tggcgctgcc ctcgttcgcg acgccgacca
     6241 ggccgctggt gcgccggatg accggtgggc cctgaccgtc acggctcacg tcgtagccga
     6301 tgagcgtgcc caccaccaac tcggccggca caccgtagag gtcgtcgctg atcgcccgga
     6361 cgaactcggt gccgccgccg gtgacgatcc cgaccgtgaa gtcgcggcgc cgcagctcgg
     6421 cgatgagttc tgacatcggc tgatagatca ccgagcgcgt cggccggccc agcgtcggat
     6481 gcgccgctct ggccatgaat tcccttgccg cggaggtgaa ttcctgcggc gtcaggccct
     6541 cgaacagacc ggccagggcg agcgcaatgc gttccagccc gatccccgcg accgccgccc
     6601 gatcaccact gagcacggcg gcgaactcgg cgcgctcgcc gatcgacggg tcctcgacca
     6661 cgcgcttccg cagcgcgtcg aggaagaagt cgagctgtac gtagctcggc cgctcgcacc
     6721 acagcgtgcc gtcgttgtcg aagtaggcga cccgatcgac gaccggcacc tcctcggccg
     6781 cggccaggaa cgcctccagc gcgtcccggg tgctgcccgg gcgccaggac ggcagcgggt
     6841 cgttgcgatc catcgcggtc gctcagctgc tgcgggtagt ggcgtcctgc aacttggcca
     6901 gcacctggtg catgttgaaa ctcgccgatt cctgccgctg cgggaactcg atcaacgtct
     6961 ccagc
//
LOCUS       DASSGQ010000020         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_32148, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000020 DASSGQ010000000
VERSION     DASSGQ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32148"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFI55_00890"
     CDS             <1..364
                     /locus_tag="VFI55_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ricin-type beta-trefoil lectin domain protein"
                     /protein_id="HET7072625.1"
                     /translation="TGYQGLCLDDRSASTANFNPIQVYTCNGTNAQSWTVNSNGTLTV
                     LGNCLDVAGAGTANGTKVDLYPCNGTGAQSWTHQSNGELVNTNSGKCLDDTGFGGSGT
                     QVQIWACADSANQQWNIP"
     gene            361..2511
                     /locus_tag="VFI55_00895"
     CDS             361..2511
                     /locus_tag="VFI55_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HET7072626.1"
                     /translation="MKRRLIAVLAAVALMAGLALASAHHAQAATTGAITGYQGLCLDD
                     RSASTANFNPIQVYTCNGTNAQQWTVAAGNTLQVLGNCLDVNGAGTANGTLVDLYPCN
                     GTGAQVWVPQSNGELLNPNSGKCLDDTGFGGSGTQMQIWACADSANQQWTLPSSGPPP
                     GNTPNFGSNVYVFTTSMSTSTIQADINAVYNTQQSNQFGTQRYELMFEPGTYNVSVPV
                     GFYTEVVGLGQNPTQTVITGGGIFANAAWNNGNATTNFWRGVENITINPSSGSTEWAV
                     SQADPMRRVQIDGNLVLDDNSSGNTTSNWSSGGFISDSVVTGQVNSGTQQQFIMRNDK
                     LGSWTGSNWNMVFVGDTGVPGQSFPSPPYTTVNQTPTVDEKPYLYIDSSGNWDVFVPS
                     NRTNAQGVSWANGNTPGTSLPISDFFIATPGSTVAQLNAALSSGQDLLFTPGIYQVNG
                     TVNVTNPDTVVLGLGIATLVSNGGNTILQTADVNGIRLGGLLFDAGTANSSVLVQIGP
                     SGSSASHAADPTVLSDVFARIGGATVGKATQTLQVNSANVVGDDLWLWRADHGNGGTV
                     GWTTNTAANGLIVNGANVTMYGLAVEHYQAVQVQWNGNGGADYFYQSEMPYDPPSQSA
                     WMDGSADGYPSINVAGGVTSFQAYGLGVYCFFNVNPSEISANALTSPATSGVQWHDMV
                     TVSLGGTGTIQHIINGTGGTVNSGSTVADLTSYS"
BASE COUNT          510 a          988 c          834 g          365 t
ORIGIN      
        1 caccgggtac cagggcctgt gcctggatga ccgctcggcg agcaccgcga acttcaaccc
       61 gatccaggtc tacacctgca acggcacgaa cgcccagagc tggaccgtga acagcaacgg
      121 caccctgacg gtcctcggca actgcctgga tgtcgccggc gccggcacgg ccaacgggac
      181 caaggtcgac ctgtatccct gcaacggcac cggcgcgcag agctggaccc accagtccaa
      241 cggcgagctg gtgaacacga actccggcaa gtgcctcgac gacaccggct tcggcggctc
      301 gggcacccag gtgcagatct gggcctgcgc cgactccgcc aaccagcagt ggaacatacc
      361 gtgaagcgcc ggctcatcgc ggtcctcgcg gcggtagcgc tgatggccgg cctcgcgctg
      421 gcgtccgctc accacgcgca ggccgcgacc accggggcga tcaccgggta ccagggcctg
      481 tgcctggatg accgctcggc gagcaccgcg aacttcaacc cgatccaggt ctacacctgc
      541 aacggcacca acgcgcagca gtggaccgtc gccgccggca acacgctgca ggtgctcggc
      601 aactgcctgg acgtcaacgg cgcggggacc gcgaacggca ccctcgtcga cctgtacccc
      661 tgcaacggca ccggcgccca ggtctgggta ccgcagtcca acggcgagct gctcaacccc
      721 aactcgggca agtgcctcga cgacaccggc ttcggcggct cgggcaccca gatgcagatc
      781 tgggcctgcg ccgactccgc caatcagcag tggacgctgc cgagcagcgg gccgcccccg
      841 ggcaacacgc cgaatttcgg gtcgaacgtc tacgtgttca ccacctcgat gtccaccagc
      901 acgatccagg ccgacatcaa cgcggtctac aacacgcagc agtcgaacca gttcggcacc
      961 cagcgctatg agctgatgtt cgagccgggc acctacaacg tctccgtccc cgtcgggttc
     1021 tacaccgagg tggtcggcct cgggcagaac ccgacccaga cggtgatcac cggcggcggg
     1081 atcttcgcca acgcggcctg gaacaacggg aacgcgacga cgaacttctg gcgcggggtg
     1141 gagaacatca cgatcaaccc gtcgtccggc agcaccgaat gggcggtctc gcaggccgac
     1201 ccgatgcgcc gggtgcaaat cgacggcaac ctcgtgcttg acgacaactc ctccggcaac
     1261 accacgagca actggtcgtc cggcggtttc atcagcgact cggtcgtcac cggccaggtc
     1321 aactcgggca cccagcagca gttcatcatg cgcaacgaca agctgggcag ctggaccggc
     1381 tcgaactgga acatggtgtt cgtgggcgac accggcgttc ccgggcagag cttcccgagc
     1441 ccgccgtaca cgacggtgaa ccagaccccg acggtcgatg agaagccgta cctctacatc
     1501 gactcatccg ggaactggga cgtgttcgtg ccctcgaacc ggacgaacgc ccagggcgtc
     1561 tcctgggcca acggcaacac gccgggaacc tcgctgccga tcagcgattt cttcatcgcg
     1621 acgccgggca gcacggtggc ccagctgaac gccgcgctca gcagcgggca ggacctgctg
     1681 ttcacccccg gcatctacca ggtcaacggc accgtcaacg tcaccaaccc cgacaccgtg
     1741 gtgctcggcc tcggcattgc cacgctggtg tcgaacggcg gcaacaccat cctgcaaacc
     1801 gccgacgtca acggcatccg cctcggcggg ctgctcttcg acgccggcac cgccaacagc
     1861 tcggtcctgg tccagatcgg cccgtccggg tcaagcgcca gccacgcggc cgacccgacc
     1921 gtgctgtcgg acgtcttcgc ccgcatcggc ggggccacgg tcggcaaggc cacccagacg
     1981 ctgcaggtca acagcgccaa cgtggtcggc gacgacctgt ggctgtggcg cgcggaccac
     2041 ggcaacggcg gcacggtcgg ctggacgacc aacaccgccg ccaacggact gatcgtcaac
     2101 ggcgccaacg tcaccatgta cgggctcgcg gtcgagcact accaggccgt gcaggtccag
     2161 tggaacggca acggcggggc cgactacttc taccagagcg aaatgccgta cgacccgccc
     2221 agccagtccg cctggatgga cggatccgcc gacggctatc cctcgatcaa cgtcgccggc
     2281 ggcgtgacca gcttccaggc ctacggcctc ggcgtgtact gcttcttcaa cgtgaacccg
     2341 agcgagatca gcgcgaacgc gctcacctcg ccggccacgt ccggagtcca gtggcacgac
     2401 atggtcaccg tctcgcttgg cggcacagga accatccagc acatcatcaa cggcaccggc
     2461 ggcacggtta actccggcag caccgtggcc gacctgacca gctacagctg agcgcaaccg
     2521 ggcggttagc accccagccg gcgcccgccc gcactcgcgg gcgggcgctc ggttgcccgg
     2581 tgaaacacgc acccgttcgg gatgatgatt ttgattttcc cgaacgggtg cgggttccac
     2641 gtcacaccaa gcgcggggcc gccgagcagg ccggcgtcca ggtgaccacg gatgttc
//
LOCUS       DASSGQ010000021         6538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_518105,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000021 DASSGQ010000000
VERSION     DASSGQ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518105"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1577)
                     /locus_tag="VFI55_00900"
     CDS             complement(39..1577)
                     /locus_tag="VFI55_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019738260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7072627.1"
                     /translation="MATPQIHEVPTELRPFSRAGISRDEHGVPHYEGLPTSLLEMLQS
                     HVSDRPDTEAVVELGGQRLTYRQLWDRAARVAGGLRAAGLQRGARVALRHPAGADWVL
                     AFWGTLMAGGIPVAINTRSAAPEVGFVVEDSDARIELSPQIPLPDAEPYVTDDLAGAD
                     IAALFYTSGTTGQPKGVPTTHQAFLTNAENIVRGPDIPRDIGAELRTLICVPLFHVTG
                     CNSQLIAATYLGGTSVIMPALDLPAMPTVLARERITLMVTVPAIYALLLGRSDLADAD
                     VSHVRWVGYGGAPIAPSLVDALRSTFANAAVFNGYGMTETSSLTTVLPDRDALAHADS
                     VGYAVPSVDIAVEPLGDDPTTGELLVRGANVTAGYWNRPEATAETITDGWLHTGDVVR
                     VDDAGRVHIIDRTKDIINRGGENISSVEVENALATAPGVAEAAVVAVPDAVMGEKVGA
                     VLFGGGPQIVIADVIAHCRERLADFKVPQYVSVSDDPLPRNPAGKLLKATLRKQVRWG
                     PPLR"
     gene            complement(1577..2548)
                     /locus_tag="VFI55_00905"
     CDS             complement(1577..2548)
                     /locus_tag="VFI55_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HET7072628.1"
                     /translation="MVEVETVRGRVSVDALGTVLMHEHVFVLSEEIWRNYPQVWDEKA
                     GIEDAVTRLQRLKALGVDTIVDPTVIGLGRDIRRIARVNERVDINIIPATGLYTYGDV
                     PNFFHFRGPGTLLGGDDPMVEMFVNDITVGIAGTPIRAAFLKCAIEEALTPGVERVMR
                     AVAETHRRTGAPIMVHTSAPHHTGLVAKEIFASEGVDLSRLLIAHSGDTTDMDYLHQL
                     LDGGAYVGMDRFGLDILLATDERVSTVAKLAAEGYASQMMLSHDACCEIDWIPRDIRE
                     RVTPNWHYTFLHDSVLPALKAAGVTAGQLDTMLVENPKAFFTPEGTN"
     gene            2747..4255
                     /locus_tag="VFI55_00910"
     CDS             2747..4255
                     /locus_tag="VFI55_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7072629.1"
                     /translation="MKLPTLPDLRAADDPMGPAVGDDDGDLNNSEFLEAVQRASATLL
                     SRGVSHGDVVAISLPNTVDFVVSLFAAWRLGAAVTPINPSLSLPEMGYQLADAAAKVL
                     ITATIPEFGSGVPAVVTTAELRQSEPSSNSDTVAPRGDTLALLIYTSGTTGRPKGVML
                     DHANLDAMCHSLIDAFSLTNTDHSLLILPLFHVNGIVAGTLSPLLAGGRATVAGRFRV
                     GTFFDRVEATRATYFSAVPTIYTMLCGLPADVAPDTSSVRFAVCGAAPASVELIEQFE
                     SRYHIPIIEGYGLSEGTCASTMNPLMGPRKAGTVGVALPGQAIRLVEVSGRPVPDGEA
                     GEVIVKGPNVMRGYLNRPEETATTIVDGWLHTGDVGRFDEDGYLVLVDRAKDMIIRGG
                     ENIYPREIEAVTHELAQIAEAAVVGRTHAVYGEEPVLFVSMYPGATLGIDAIDAHLRN
                     QLSKYKLPAQITVMEALPKNPVGKLDKPALRRLLSNPPATETRHATTAPEQS"
     gene            complement(4352..5578)
                     /locus_tag="VFI55_00915"
     CDS             complement(4352..5578)
                     /locus_tag="VFI55_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007530977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7072630.1"
                     /translation="MSLFDISQRAQELQAELLDFVESYVYPAEGIYDDQMRESGNPHH
                     QPPIIDELKVEARKRGLWNLFHPHPGTGAGLTNLEYAPLAEIMGRSFIASEACNCSAP
                     DTGNMEVLELFGTEEHREKYLKPLLEGTIRSAFAMTEPRVASSDATNIELSMVRDGGD
                     YVLNGRKWFASNAMHQNCKVLIVMGKTDPAAAPHRQQSMMVVPIDAPGVTVLRSLPVF
                     GYMDREGHAEIEFTDVRVPAKDVLKGEGEGFAISQARLGPGRIHHCMRAIGMAERALQ
                     LMCKRAQSRVTFGKPIADNANIQDWIAEARIDIEMIRLLTLKAAHMMDTVGNKQAQTE
                     IAAIKVAAPNIALKIIDRAIQVHGGAGVTDDFPLALAYAHLRTLRLADGPDEVHKRAI
                     ARRELRQYRDLRPEGL"
     gene            complement(5588..6406)
                     /locus_tag="VFI55_00920"
     CDS             complement(5588..6406)
                     /locus_tag="VFI55_00920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007300614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7072631.1"
                     /translation="MKVTGKVAIVTGGGGGIGGALAEELTARGARVVVADLGAAGAQA
                     VTDKINATSPGSAVAAGADVSDTAAIRGLITLAEQQFGPVDLYFANAGIVGVPGLEAS
                     EAEWEQSIDVNLRAHIRAAQLLIPGWVERGEGYFVSTASAAGLLTQIGSATYSVTKHA
                     AVGFAEWLAIAYGDQGVRVSCLCPMGVNTELLYAGEKTGDPRGVAATRAVTSAGEVIE
                     PDQVARDVLAAIDEERFLILPHAAVLDMFRHKAGDYERWLRGMRRYQAELLNNI"
     gene            complement(6403..>6538)
                     /locus_tag="VFI55_00925"
     CDS             complement(6403..>6538)
                     /locus_tag="VFI55_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019745707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET7072632.1"
                     /translation="VMVDDLQQNLDGAARLGIAGVLHTDAADTGEQLGERFGIGVRTR
                     "
BASE COUNT         1071 a         2122 c         2139 g         1206 t
ORIGIN      
        1 tgcgtccctc acgtcgtaac cgtctgcggc gcgggtggtc atcgcaacgg cggtccccac
       61 cgcacctgtt tgcgcagcgt ggccttcagc agcttgcccg cggggttgcg gggcagcggg
      121 tcgtccgaaa cgctcacgta ctggggaact ttgaagtcgg cgagccgttc gcggcaatgt
      181 gctatgacgt cggcgatgac gatctggggg ccgcctccga agagaaccgc cccgactttt
      241 tcacccatca ccgcatctgg caccgccacc actgctgctt cggcgacacc gggggcggtc
      301 gcgagcgcat tttccacctc cacactggag atgttctcac ccccgcggtt gatgatatcc
      361 ttcgtccggt cgatgatgtg cacgcgtccg gcgtcgtcga ccctgaccac gtctccagtg
      421 tgcagccagc cgtcggtgat ggtttcggcg gtggcctcgg ggcgattcca gtagcccgcc
      481 gtgacgtttg ccccgcgcac caacaactcg ccggtggtgg ggtcgtcgcc gaggggctcg
      541 accgcgatgt ccaccgatgg cacggcgtag ccaaccgaat cggcatgcgc gagggcgtcg
      601 cggtcgggca gcacggtggt caacgaggag gtttcggtca tcccgtagcc gttgaacacc
      661 gcggcgttgg cgaacgtcga ccgcagcgca tccaccaacg atggcgcgat cggcgcgccg
      721 ccgtatccca cccatcggac atggctgacg tcggcgtcgg cgaggtcgga ccgaccaagc
      781 agcagggcgt agatggccgg cacggtcacc atcagcgtga tccgttcccg cgcaagcacc
      841 gtcggcatcg cgggaagatc caacgccggc atgatcaccg aggtcccgcc caaatatgtc
      901 gcggcgatta gttgcgagtt gcagcccgtc acatgaaata gcggcacgca gataagtgtc
      961 cgcagctcgg ccccgatatc acggggaatg tccgggccgc gaacgatgtt ctcggcgttg
     1021 gtcagaaagg cctggtgggt ggtcggcacg cccttcggct gccccgtggt tcccgacgtg
     1081 tagaacaacg cggcgatatc ggcaccggcg aggtcgtcgg tgacatacgg ctcggcgtcg
     1141 ggcaggggga tctgtggcga cagttcgatc cgggcgtcgc tgtcttctac gacgaaccca
     1201 acctcggggg cggccgagcg ggtgttgatc gccaccggga tcccacccgc catgagggtg
     1261 ccccagaacg ccagcaccca gtcggctcca gccggatggc gcagcgccac ccgggccccg
     1321 cgttgcagcc cggccgcgcg caatccgccc gcgacccgcg cggcgcggtc ccacaactgg
     1381 cggtaggtca gtcgctggcc gcccaattcg acgacggcct cggtgtcggg acggtcgctg
     1441 acatgactct gcagcatttc cagcagtgag gtcggcagcc cttcgtagtg cggtacgccg
     1501 tgttcgtcgc gggagatccc tgccctggaa aacggtctca gctcggtggg cacctcgtgg
     1561 atctgcggcg tggccatcag ttcgtccctt ccggggtgaa aaacgctttg gggttttcca
     1621 ccagcatcgt gtcgagctgc ccggcggtca cgccggcggc tttcagggcc ggcagtaccg
     1681 agtcgtggag gaaggtgtag tgccagttcg gtgtcactcg ctcgcggata tcccttggta
     1741 tccagtcgat ttcgcagcac gcgtcgtggg acagcatcat ctggctcgcg tagccctcgg
     1801 cggccagttt cgccacggtc gacacccgct catcggtggc cagcaggata tccaggccga
     1861 accgatccat gccgacatag gcaccgccat cgagcagttg gtgcaggtag tccatgtcgg
     1921 tggtgtcgcc gctgtgggcg atgagcagcc gggacagatc aacgccctcc gatgcgaaga
     1981 tttctttggc caccaagccg gtgtggtggg gtgcgctggt gtggaccatg atcggcgccc
     2041 cggtgcggcg gtgggtctcg gccacggcac gcatcacgcg ttcgacgccg ggggtcagcg
     2101 cctcttcgat cgcgcatttg aggaacgcgg ccctgatcgg ggtgccggcg atcccgacgg
     2161 tgatgtcgtt gacgaacatc tccaccatcg ggtcatctcc gcccagcagc gtgcccgggc
     2221 ctcggaaatg aaaaaagttg ggcacatcgc cataggtgta caacccggtc gccgggatga
     2281 tgttgatgtc gactcgttcg ttgacccggg caatgcgacg aatgtcgcga ccgagcccga
     2341 tcacggtcgg gtcgacgatc gtgtcgaccc caagtgcttt gagcctctgc aggcgtgtca
     2401 ccgcgtcttc gatgccggcc ttctcatccc atacctgtgg gtaattgcgc cagatttcct
     2461 cgctgaggac gaacacgtgc tcatgcatca gcaccgtgcc caacgcatcc accgacactc
     2521 ggccccggac cgtctccacc tcgaccatcg tgtttgtctc ctgcttgccg ggcgggcatc
     2581 accgcttccc gctgccgccg cgagtacctg agaataccag aatagatatc gatattgaat
     2641 tcggcttcat attgttctag gtgcttgacc tgggctatgg tccagaaatg cctctcagaa
     2701 tcgttacgag tcgcccggcc gccaaaggta acgaggagcc catcgcatga aattgccgac
     2761 cttgccggat ctgcgcgcag cggacgaccc gatggggcca gccgttggcg acgacgacgg
     2821 cgatctcaac aacagcgagt tcctggaggc cgtccagcgg gccagcgcga cgctcctcag
     2881 tcgcggggta tctcacggcg acgtggtggc gatctcgcta cccaacaccg tcgattttgt
     2941 ggtgtcgctg tttgcagcgt ggcggttagg cgcggcggtc acaccgatca atccgtcgtt
     3001 gtcgctgccg gagatgggct atcagctcgc cgacgccgct gccaaggttt tgatcaccgc
     3061 gaccattccc gaattcggct cgggcgtacc ggcggtcgtg acaacagccg agctgcggca
     3121 gagtgagccc agttcgaact ccgataccgt cgcccctcgc ggcgatacac tcgcactgct
     3181 gatctacacc agcggaacga cgggtcgccc caagggtgtg atgctcgatc acgccaacct
     3241 ggatgcgatg tgccacagct tgattgatgc gttctcgctg accaacaccg accacagcct
     3301 gctcatcctg ccgctttttc acgtcaacgg gattgtggcc ggcaccctgt caccgctgct
     3361 ggccggaggc cgcgccaccg tcgccggacg gttcagagtc ggcacgttct tcgaccgggt
     3421 tgaggccacg cgcgcgacct atttttcggc agttccaacg atttacacga tgctgtgcgg
     3481 cctgcccgcc gatgtggcac cagacacgtc atcggtgcgg ttcgcggtct gtggggccgc
     3541 tccggccagc gtcgagctga tcgagcagtt cgaatcgcgc tatcacattc caatcatcga
     3601 aggctatggc ctgtcggaag gaacatgcgc atcaacgatg aacccgctga tgggcccgcg
     3661 caaggccgga acggttgggg ttgcgctgcc cggtcaggcg attcgactgg tcgaggtatc
     3721 gggtcgcccg gtgcccgacg gtgaggccgg cgaggttatc gttaaggggc ccaacgtgat
     3781 gcgcggctac ctcaaccggc ctgaggagac cgccacgacg atcgtcgacg gttggctcca
     3841 taccggcgat gtcggccgct ttgacgagga cggctacctg gtactcgtgg accgcgctaa
     3901 ggacatgatc atccgcggcg gtgagaacat ttacccccgc gagatcgaag ctgtcacaca
     3961 cgaactggcg cagatcgccg aagccgcggt cgtcggccga acgcacgccg tctacggcga
     4021 ggagcccgtg ctattcgtct cgatgtaccc cggcgccacc ctgggcatcg acgcgatcga
     4081 tgcccatctg cgcaatcagc tgtcgaaata caaactacca gcacagatta cggtgatgga
     4141 agcgcttccc aagaaccccg tcggcaagct cgataaaccc gcattgcgcc ggctgctgag
     4201 caacccaccg gcaaccgaaa cccgccacgc cacgacggcg cccgagcaga gctgagcgcg
     4261 tcggatattg aggccgtttc ctgaaatacg cgctggcgca acctggcaac gtgcccgacg
     4321 gggcaaaccg gcgcacccca cgcgctgctc gtcacagccc ctccggccga aggtcgcgat
     4381 attggcgtag ctcacggcgc gcgatcgcgc gcttgtgcac ttcgtccggc ccgtcggcca
     4441 atcgcagcgt gcgtaggtgg gcgtaggcca gggcgagtgg gaagtcgtcg gtgacgccgg
     4501 cgccgccgtg cacctggatg gcgcggtcga tgatcttcag cgcgatattc ggggcggcga
     4561 ccttgatggc cgcgatctcg gtctgcgctt gcttgttgcc caccgtgtcc atcatgtggg
     4621 cggccttgag ggtgagcaga cggatcatct caatgtcgat gcgcgcctcg gcaatccagt
     4681 cctggatgtt ggcgttgtcg gcgatcggtt tgccgaaggt gacccgggac tgggcgcgtt
     4741 tgcacatcag ctgtagcgcg cgttcggcca tgccgatggc ccgcatgcag tggtggatgc
     4801 ggccaggccc gagccgggcc tggctgatcg cgaaaccctc accctcaccc ttgagcacgt
     4861 ctttggcggg cactcgcacg tcggtgaact cgatctcggc gtggccctcg cggtccatgt
     4921 agccgaacac cggcaggctg cgcaacacgg tgaccccggg cgcatcgatc gggacgacca
     4981 tcatcgactg ctgacggtgc ggtgcggcgg cgggatcggt cttgcccatc acgatcaaga
     5041 ccttgcagtt ttggtgcatc gcgttggagg cgaaccactt tcgcccgttg agcacatagt
     5101 cgccaccgtc gcgcaccatg gacagttcga tgttggtggc gtccgagctg gctaccctcg
     5161 gctcggtcat cgcgaacgct gatctgatgg tgccctccag cagcggcttg aggtacttct
     5221 cgcggtgctc ctcggtgccg aacagttcga gcacctccat gttgcccgtg tcgggggcgc
     5281 tgcagttgca agcctcggag gcgatgaagc ttcggcccat gatttcggcc agcggcgcgt
     5341 attcgaggtt cgtcaggcca gcgccggtgc cggggtgcgg gtgaaacagg ttccacaggc
     5401 cgcgttttcg cgcctcgacc ttgagttcgt cgatgattgg cggctggtgg tgcgggttgc
     5461 cggattcacg catttggtcg tcgtatattc cctcggcggg gtagacgtac gattccacga
     5521 agtcgagcag ctcggcctga agctcttgcg cgcgttggga aatgtcaaac agggacatgg
     5581 aaactcctca gatgttgttc agcaactcag cttggtaacg gcgcatgccg cgcagccagc
     5641 gctcgtaatc gccggccttg tgccggaaca tgtccagcac ggcggcgtgc ggcaggatca
     5701 ggaaacgctc ctcgtcgatg gcggcgagga cgtcgcgggc gacctggtcg ggttcgatga
     5761 cctcgcccgc cgacgtgacg gcgcgagtgg cggcgacgcc gcgggggtct ccggtcttct
     5821 cgccggcgta gagcagttcg gtgttgacgc ccatcgggca caggcaactg acccgcacgc
     5881 cctggtcgcc gtacgcgatc gcaagccatt cggcgaatcc gacggcggcg tgcttggtga
     5941 cggagtaggt cgccgatccg atctgggtga gcaggccggc ggccgatgcg gtgctgacga
     6001 agtagccctc gccgcgttcc acccagccgg ggatcagcag ctgcgcggcc cggatgtgcg
     6061 cgcgcaggtt cacatcgatc gattgttccc actccgcctc gctggcttcc aggccgggta
     6121 ccccgacgat cccggcgttg gcgaagtaca ggtcgaccgg gccgaactgc tgttccgcca
     6181 acgtgatcag gccgcgaatg gccgccgtgt cggacacgtc cgcgccggct gcgaccgccg
     6241 aacccggtga tgtggcgttg atcttgtcgg tgacggcttg cgcgccggcc gcgccgaggt
     6301 cggccaccac tacccgggcg ccgcgggcgg tgagttcctc agcgagcgcg ccgccgatcc
     6361 cgccgccacc accggtgacg atggcgacct tgcctgtgac cttcatcgag tcctcactcc
     6421 tatgccgaag cgttcgccga gttgttcgcc ggtgtcggcg gcgtcggtgt gcagcacgcc
     6481 ggcgatcccg agccgtgcgg ccccgtcgag attttgctgt agatcgtcga ccatgacc
//
LOCUS       DASSGQ010000022        12539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1429492,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000022 DASSGQ010000000
VERSION     DASSGQ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12539
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429492"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            457..771
                     /gene="mihF"
                     /locus_tag="VFI55_00930"
     CDS             457..771
                     /gene="mihF"
                     /locus_tag="VFI55_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HET7072633.1"
                     /translation="MALPQLTDEQRAAALEKAAAARRARAELKDRLKRGGTNLTQVLK
                     DAESDEVLGKMKVSALLEALPKVGKVKAQEIMTELEIAPTRRLRGLGDRQRKALLEKF
                     GS"
     gene            834..1454
                     /gene="gmk"
                     /locus_tag="VFI55_00935"
     CDS             834..1454
                     /gene="gmk"
                     /locus_tag="VFI55_00935"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET7072634.1"
                     /translation="MSAGGGPDVEQRARPASKGRVVVLSGPSAVGKSSVVRRLRERIP
                     NLQFSVSATTRAPRPGEVDGVDYHFVSPDRFQQLIDEGALLEWAEIHGGLHRSGTPAE
                     PVRAATEAGLPVLIEVDLAGARAVKQALPEAITVFLAPPSWEDLKARLVGRGTETPEA
                     MRRRLDTARTEMAAQDDFDEVVVNSRLESACAELVSLLVGTAPGPA"
     gene            1565..1888
                     /gene="rpoZ"
                     /locus_tag="VFI55_00940"
     CDS             1565..1888
                     /gene="rpoZ"
                     /locus_tag="VFI55_00940"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET7072635.1"
                     /translation="MTIPQSDASLTAVDQFDPSAGGQGAYDTPLGITNPPIDELLDRV
                     SSKYALVIYAAKRARQINDYYNQLGEGILEYVGPLVEPGLQEKPLSIALREIHADLLE
                     HTEGE"
     gene            1909..3159
                     /gene="coaBC"
                     /locus_tag="VFI55_00945"
     CDS             1909..3159
                     /gene="coaBC"
                     /locus_tag="VFI55_00945"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HET7072636.1"
                     /translation="MDLKRIVVGVSGGIAAYKACTVVRQLTEAGHQVRVIPTESALRF
                     VGAATFEALSGQPVHIGVFDDVPEVPHVQLGKQADLVLVAPATADLLARAAAGRADDL
                     LTATLLTARCPVLLAPAMHTEMWLHPATVDNVATLRRRGAVVLEPASGRLTGADSGAG
                     RLPEAEEITTLAQLLLERHDALPYDLAGWRLLVTAGGTREPIDPVRFVGNRSSGKQGY
                     AVARVAAQRGAEVTLIAGHTTGLIDPAGVDVVHVSSAQQLGEAVSKHAPQSDVLVMAA
                     AVADFRPARVATAKIKKGPDGQDGPPPIELVRNDDVLAGTVRGRAHGQLPNMRAIVGF
                     AAETGDTNGDVLFHAREKLRRKGCDLLVVNAVGDNKAFEVDSNDGWLLASDGTESALE
                     HGSKTLMASRIVDAIVTFLHGEGE"
     gene            3278..4489
                     /gene="metK"
                     /locus_tag="VFI55_00950"
     CDS             3278..4489
                     /gene="metK"
                     /locus_tag="VFI55_00950"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET7072637.1"
                     /translation="MSEKGRLFTSESVTEGHPDKICDAISDSVLDALLAQDPRSRVAV
                     ETLVTTGQVHVVGEVTTTAKEAFADITNTVRERILEIGYDSSDKGFDGASCGVNIGIG
                     AQSPDIAMGVDTAHEARVEGAADPLDAQGAGDQGLMFGYAISDTPELMPLPIALAHRL
                     SRRLTEVRKNGVLPYLRPDGKTQVTIAYEDDVPVGLDTVVISTQHAADIDLENTLTPD
                     IREKVLNTVLDDLAHETLDTSSTRLLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWA
                     RHGGGAFSGKDPSKVDRSAAYAMRWVAKNIVAAGLAERVEVQVAYAIGKAAPVGLFVE
                     TFGTATVDPVKIEKIVPEVFDLRPGAIIRDLDLLRPIYAQTAAYGHFGRTDIDLPWEQ
                     LNKVDELRRAV"
     gene            complement(4560..5129)
                     /locus_tag="VFI55_00955"
     CDS             complement(4560..5129)
                     /locus_tag="VFI55_00955"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET7072638.1"
                     /translation="MAKEFGRIDESLRDFIADQAMFFVATAPSQGGRINLSPKGYRDT
                     FAVLDEHTVAYLDLFGSGVETIAHLRDNGRITIMFCSFTRNSRILRLFGAGRVVRPDD
                     AEFACLLTHFGTQHAGVRAVMVIDVERIADACGFAVPYYELVNERPVLDAHHTKASDE
                     GFAGMADRNRHSIDGLPALEPDHPPPRRR"
     gene            complement(5161..6123)
                     /locus_tag="VFI55_00960"
     CDS             complement(5161..6123)
                     /locus_tag="VFI55_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7072639.1"
                     /translation="MIEPALAPPGIDPTFKMLLDAFPMTFNAADGVEVARSRLRLLKV
                     PPELVPDVRTEERTIGYGEFTDIPVRIYWPASDDTPLPVVVFYHGGGFSLGDLDTHDP
                     VARLHAVSADAIVVSVDYRLAPEHPFPAGVNDCWAALQWVAENAAELGGDPKRIAVAG
                     DSAGGNLAAVMAHLARDNGGPELVFQLLWYPSVTADLSLPSHTENASAPILDRDVIDA
                     FLSWYVPDLDITNPKDVPTTLAPANAADLSGLPPAYIGTAEHDPLRDDGARYADLLNA
                     AGVPTELNNESTLVHGYVNFALVIPAAGEATHRGLTALRKALHP"
     gene            complement(6128..7111)
                     /locus_tag="VFI55_00965"
     CDS             complement(6128..7111)
                     /locus_tag="VFI55_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7072640.1"
                     /translation="MPSLDSTADGKPGIDPILLKVLDAVPFRLTTDDGVAVARQRFRD
                     LPRLPLHPEVRVEDRAIPGPAGPIGIRIYWPSTYSAGGAPAPVVLYFHGGGFVVGDLE
                     THDGSARQHAAGADAIVVAVDYRLAPEHPYPAAVEDAWAATLWVAAHGPDIGADTTRM
                     AVAGDSAGGTIAAVMAQRARDGGGPPIVFQLLWYPSTMWDPSLPSFTENADAPILDVK
                     AVTALSLWYAGEVDLSDPPPGMAPGRAENLGDLAPAYIAVAGYDPLRDDGIRYGELLA
                     AAGVPVEVHNAETMVHGYLGYAGVVPAATAELERGLAALGDTLHKREGSVR"
     gene            7110..7841
                     /locus_tag="VFI55_00970"
     CDS             7110..7841
                     /locus_tag="VFI55_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HET7072641.1"
                     /translation="MEVPYAPRMVSGAWVVAGWAGVGYGVYLTVLALRTPPGSALTGH
                     WVMQPPFKALMALLLTVAAVAHPIVRERRWLMPALLLSALGDWVLAIPWWTLSFVVGL
                     VSFLLAHLCFLAALFPLARASRVRLAAVVLMCLATVSLLAWFWPHLGRDQLTIPVTLY
                     MAVLTAMVCAALLADLPTIWAAVGAVCFAASDAMIGIDRFILYNDALAVPIWWLYAAA
                     QILITAGFFFGRQERDEPELEPFAG"
     gene            7976..9913
                     /locus_tag="VFI55_00975"
     CDS             7976..9913
                     /locus_tag="VFI55_00975"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013829229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET7072642.1"
                     /translation="MLSVPHLDREFDYLVSPEQSDDAQPGVRVRVRFHGRLVDGFVLE
                     RRNDTDHAGKLGWLDRVVSPEPVLTAETRRLVDAVAARYAGTRPDVLRLAVPARHARV
                     ERESTAVVPLPVVAPVDPSGWESYGRGGQFLAALAESRAARAVWQVLPGEPWADRFAE
                     AAAQTVRTGRAVLAIVPDQRDLDALWRAATTRIDEASVVALSAGLGPAARYRRWLAAL
                     RGTARVVIGTRSAVFAPLSDLGLVMVWSDADDSLAEPRAPYPHAREVAMLRAHQARCA
                     GLIGGYARTAEAHALVRSGWAHDIVAARPAVRARTPRVVALDDTGYADERDPAARTAR
                     IPSIALRAARSALEAGAPVLVQVPRRGYVPSLACGRCRTIARCRHCTGPLSLQEHGAV
                     CRWCGRVEPAPRCARCGSDAVRAVVVGARRTAEELGRAFAGTPVVTSSGEAVVSEVAA
                     QPALVVATPGAEPHAAGGYGAALLLDCWALLGRQDLRAAEDALWRWMSAAALVRARGD
                     GGVVTVVADASIPTVQALIRWDPVGHAEAELVARTEVGLPPSVQMAAIDGAAGAAEAL
                     LGEARLPDGAELLGPVDLPPGVRRPPGTPTDVPAIRMLVRVRREQGLQLAAALRRGVG
                     VLSARQSHEPVRVQIDPLHIG"
     gene            10070..10558
                     /locus_tag="VFI55_00980"
     CDS             10070..10558
                     /locus_tag="VFI55_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7072643.1"
                     /translation="MATDNATTADESLDAITDALLTASRLLMAISARSISQVDETITI
                     PQFRTLVILSSRGPINLATLAGLLGVQPSATGRMVDRLVAAGLIDRLPHPTSRRELLA
                     ALTKRGRDVVRRVIAYRRAEIAAIVAKMPPTERRGLVRALGAFTAAGGEPDVQLDVDI
                     DL"
     gene            10654..11583
                     /gene="fmt"
                     /locus_tag="VFI55_00985"
     CDS             10654..11583
                     /gene="fmt"
                     /locus_tag="VFI55_00985"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET7072644.1"
                     /translation="MRLVFAGTPEPALPALRRLIESPRHQVVAVLTRPDAAAGRHRKP
                     EPSPVAREALDRGIPVLRPARPNSPEFVAELSELAPQCCAVVAYGALLRDDLLAVPPQ
                     GWINLHFSLLPAWRGAAPVQAAIAAGDAITGATTFQIEPSLDSGPVYGVVTETIRPTD
                     TAGDLLERLAHSGAALLSATLDGVADGTLMPRPQPADGVSIAPKITVEQARVRWDLPA
                     PVVERRIRAVTPNPGAWTLIGDLRIKLGPVGLDQPPKPLPPGAIHVDRHSVWIGTGTE
                     PVRLGQIQPPGKKLMNAVDWARGARLDTAVRAS"
     gene            11580..>12539
                     /locus_tag="VFI55_00990"
     CDS             11580..>12539
                     /locus_tag="VFI55_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET7072645.1"
                     /translation="MSPPRPSAKGRGKPRRPLDPARAAALDVLRAVRERDAYANLALP
                     ALLRERGISGRDAAFATELTYGTCRTQGLLDAIIGAAAGRSPEAIDPVLLDLLRLGAY
                     QLLRTRVDAYAAVSTTVEQAGIEFDSARAGFVNGVLRTIAARDEKSWMDELAPDPVRD
                     PIGHAAFVHAHPRWIAQAFADALGPDAAELDAVLASDDERPRVHLAARPGLLTAAELA
                     DAVGGTVGTYSPYAVYLPGGDPGRLAPVRDGAALVQDEGSQLVARALTLAPVEGDTGR
                     WLDLCAGPGGKTALLAALGVASGARVTAVEPAPRRADLVDENTR"
BASE COUNT         1863 a         4259 c         4514 g         1903 t
ORIGIN      
        1 gtagaccgca ccccggaaat ttcgctgggc acggccccgc gagcgcgccg acctgccgct
       61 tgaccaccga cgcgacacgc gcgacacgcc acgagaaatc cgtgatccac ggcacccggg
      121 ctcccggcgg gccgctgccg gcgtcagacg gcgcccaccg ccgcggcggc ccgcgctcgg
      181 ccgtgagcct aaaactgctg gtagaagcca tgatttgggt caatgcgagg accacgccgt
      241 cgccggagcg gcggcgtgcc gaaccggcgt caacggtgtc tcacggaccg cgtgcgggcc
      301 ccacccgccg gtcccacgct gggcgtttac cccatcaacc agggggtcgg attagatttc
      361 gtcaggaagc gtgagtacgg tcgtctgcgc tggctaaatg gcccggcagg gacagaagag
      421 atcgattgag atcgattgat cagatatgga ggaatcgtgg cccttcccca gttgaccgac
      481 gagcagcgcg cggccgcgtt ggagaaggcg gctgccgcac gtcgagcgcg agcagagctc
      541 aaggaccggc tcaagcgcgg tggcaccaac ctgacgcagg tgctcaagga cgccgaaagc
      601 gatgaagtcc tcggcaagat gaaggtgtct gcgctgctgg aggcgttgcc caaggtgggc
      661 aaggtcaagg cgcaggaaat catgaccgag ctggaaatcg cccccacccg tcgcttgcgc
      721 ggcctgggtg accggcagcg caaggccttg ctggaaaagt tcggctccta gccccgccac
      781 ccgacgatgc aggccggacg gcctgagttg ggggtaccga cccggggtcg ccggtgagcg
      841 ccggcggggg accggacgtc gagcagcgcg cacgtcccgc gtcgaagggg cgtgtggtcg
      901 tgctgtccgg tccctccgcg gtcggcaagt catcggtggt ccgccgcctg cgtgagcgga
      961 ttcccaacct gcagttcagc gtgtcggcga cgactcgggc cccgcggccc ggtgaggtcg
     1021 acggcgtcga ctaccacttc gtcagccctg accggtttca gcaactgatc gacgagggcg
     1081 cgctgctgga atgggccgag atccacggcg ggctgcaccg atcgggcacg ccggccgagc
     1141 cggtgcgggc agccaccgag gccgggcttc cggtgctcat cgaggtcgac ctggccgggg
     1201 cccgggcggt caagcaagcg ctgccggagg ccatcacggt gttcctggcg ccacccagct
     1261 gggaggatct gaaggcgaga ttggtcggcc gtggcaccga gacgccggag gcgatgcggc
     1321 gccgcctcga caccgcccgg accgaaatgg cggcccaaga cgacttcgac gaggtggtgg
     1381 tcaacagtcg attggagtct gcgtgcgccg aattggtatc gttgctggtg ggaactgcgc
     1441 cgggcccggc ataaatcgtc cggccagacc aggagccaga ccagattcaa tcgagatcaa
     1501 gcatcgagtg tctaaccgtt ttagccgctc agatggcctt atagccgcca ggagattgac
     1561 ttacgtgact attccccagt ccgacgcgtc gctgaccgcc gtggaccagt tcgatccgtc
     1621 ggcaggtgga caaggcgcct acgacacccc gctgggcatc accaacccgc ccatcgacga
     1681 gctgctcgac cgcgtgtcga gcaagtatgc gctggtgatt tacgcggcca agcgggcgcg
     1741 gcagatcaac gattactaca accagctcgg cgagggcatc ctcgagtacg tcggcccgct
     1801 ggtcgagccc gggctgcagg agaagccgct gtccatcgcg ttgcgcgaga tccacgccga
     1861 ccttctcgaa cacaccgagg gcgagtaacg ggcagggccg ggcgcgctgt ggacctcaag
     1921 cggatcgtcg tcggcgtctc cggaggtatc gccgcctata aggcgtgcac cgtcgttcgt
     1981 caactcaccg aggccggtca ccaggttcgt gtcatcccca ccgaatccgc actccggttt
     2041 gtcggggccg ccaccttcga ggcactttcg ggccagccgg tgcacatcgg cgtctttgac
     2101 gacgttcccg aggtgccgca cgtccagctc ggcaagcagg ccgacctggt cctggtggcg
     2161 ccggccaccg cggacctgct cgcccgcgca gcggcgggcc gggccgacga cctgctgacc
     2221 gccaccctgc tcacggcccg gtgtccggtg ctgctcgcgc cggcgatgca caccgagatg
     2281 tggttgcatc cggccaccgt cgacaacgtg gccacgctgc ggcgccgtgg ggcggtggtg
     2341 ctcgagcccg catccgggcg gctcaccggg gccgacagcg gggcgggcag actgccggag
     2401 gccgaagaga tcaccaccct ggcccagctg ctcctggagc gccacgacgc gctgccgtac
     2461 gacctcgccg gctggcggct gctggtcacg gccggcggca cccgggaacc gatcgatccg
     2521 gtgcgcttcg tcggcaatcg cagctccggc aagcagggct acgcggtggc gcgggtcgcc
     2581 gcgcagcgcg gcgccgaggt cacgttgatc gcggggcaca ccaccggcct gatcgacccc
     2641 gccggtgtgg acgtggtcca cgtcagctcg gcgcagcaac tcggcgaggc ggtgtccaag
     2701 catgcccccc agtcggacgt gctggtgatg gcggccgccg tcgccgactt ccggccggcg
     2761 cgtgtcgcca cggccaagat caagaaaggc ccagacggcc aggacgggcc gccgccgatc
     2821 gaactggtgc gcaacgatga cgtgctggcc ggcacggtgc gggggcgcgc gcacgggcag
     2881 ctgcccaaca tgcgtgcgat cgtcggcttc gccgccgaga ccggcgacac caacggcgat
     2941 gtgttgttcc acgcccggga aaagctgcgt cgcaagggct gtgacctgtt ggtggtcaac
     3001 gccgtcggcg acaacaaggc atttgaggtg gacagcaacg acggctggct actggcatcc
     3061 gacggtaccg agtcggcgct ggaacacggc tccaagacgc tgatggcgag tcgtatcgtg
     3121 gatgcgatcg tcacctttct gcacggcgag ggcgagtaga ggggtctagc tttatagcgc
     3181 ctgggggggt gcggggtgaa ccctgcccga gcgggcggac taagacggat gccggccgat
     3241 atgattcggc taactaacta ttggaaggat cgggattgtg agcgaaaagg gtcgtctgtt
     3301 taccagtgag tcggtgaccg aagggcaccc cgacaagatc tgtgacgcga tcagcgactc
     3361 ggtcctcgac gcgcttcttg cgcaggaccc gcgctcacgt gtcgcggtcg agacgctggt
     3421 gaccaccggg caggtgcacg tggtggggga agtgaccacg acagccaagg aggcgttcgc
     3481 cgacatcacc aacacggtcc gcgagcgcat cctcgagatc ggctacgact cgtcggacaa
     3541 gggcttcgac ggggcgtcgt gcggggtgaa catcggcatc ggcgcgcagt cgcccgacat
     3601 cgcgatgggt gtggacaccg cccacgaggc acgcgtggag ggcgcggcgg atccgctgga
     3661 cgcccagggc gccggtgacc agggcctgat gttcggctac gcgatcagcg acaccccgga
     3721 gctgatgccg ctgcccatcg cgctggccca ccgcctgtcg cggcgcctga ccgaggtccg
     3781 caagaacggc gtgctgccct acctgcgccc cgacggcaag acccaggtga ccatcgccta
     3841 cgaggacgac gtcccggtcg ggttggacac cgtggtgatc tccacccagc acgctgccga
     3901 tatcgacctg gaaaacacgc tcacgcccga catccgggaa aaggtcctca acaccgtgct
     3961 cgacgacctc gcgcacgaaa cgctggacac gtcctcgacg cggttgctgg tcaacccgac
     4021 cggcaagttc gtcctcggag ggcccatggg cgacgccggg ctgaccggcc gcaagatcat
     4081 cgtcgacaca tacggcggct gggcccggca cggcggcgga gccttctccg gcaaggatcc
     4141 gtccaaggtg gaccggtcag cggcgtacgc gatgcgctgg gtggccaaga acatcgtcgc
     4201 cgccggcctg gcggagcggg tcgaggtgca ggtggcctac gccatcggca aagccgcgcc
     4261 ggttggcttg ttcgtcgaga cgttcggcac cgcgacggtt gacccggtca agatcgagaa
     4321 gatcgttccc gaggtgttcg acctgcggcc cggcgccatc atccgcgacc tggacctgct
     4381 gcgcccgatt tacgcgcaga ccgccgcgta cgggcacttc ggccgcaccg acatcgacct
     4441 gccgtgggag cagctgaaca aggtcgacga gctcaggcgc gccgtctagg tttcgcactc
     4501 gaacgtgacg ctgcggtcac gttcggcgcc cgatgtgact gcagcgtcac gttcggcggt
     4561 cagcgccgtc taggtggcgg gtggtcgggc tccagcgcgg gcagcccgtc gatgctgtgc
     4621 cggttgcgat cggccatccc agcgaatccc tcatcggacg ccttggtgtg atgcgcgtcg
     4681 agcaccggcc gttcgttgac gagctcgtag tagggcaccg cgaatccgca ggcgtcggcg
     4741 atccgctcga cgtcgatcac catgacggcg cgaacgcccg catgctgggt cccgaaatgc
     4801 gtgagcaggc aggcgaattc ggcgtcatcc gggcggacga cccgcccggc gccgaacagc
     4861 cgcaagattc gcgagttgcg ggtgaatgag cagaacatga tggtgatccg gccgttgtcg
     4921 cgcaggtggg cgatcgtttc aacgccgctg ccgaacaggt cgagataagc cacggtgtgc
     4981 tcgtcgagca ccgcgaaggt gtcgcgatat cccttggggg agaggttgat ccggccgccc
     5041 tgtgagggcg cggtggcgac gaagaacatg gcctgatctg cgatgaagtc ccgcagggac
     5101 tcgtcgatcc gcccaaactc cttggccatc ggtcgagcct agcgcccggc caccgcacgc
     5161 ctaggggtgc agcgccttgc gcagcgccgt cagcccgcga tgggtggcct cgccggcggc
     5221 gggtatcacc agggcgaagt tgacgtaacc gtgcaccagg gtcgactcgt tgttcagttc
     5281 ggtgggcacg ccggcggcgt tgagcaggtc ggcgtagcgg gccccgtcgt cgcgcagtgg
     5341 atcgtgttcc gcggttccga tgtaggcggg cggcaaaccg gacaagtcgg ccgcattggc
     5401 cggcgccagc gtggtgggta cgtcctttgg attggtgatg tcgagatcgg gcacatacca
     5461 ggacaggaag gcgtcgatga cgtcgcggtc caggatgggc gcagaggcgt tctcggtgtg
     5521 cgacggcagc gacagatcgg ccgtgaccga cgggtaccac agcaactgaa acacgagttc
     5581 cggcccgccg ttgtcgcggg ccaggtgcgc catcaccgcg gccaggttgc cgcccgccga
     5641 gtcgccggcc accgcgatcc gcttggggtc accgcccagc tcggcggcat tttcggcgac
     5701 ccactgcaag gcggcccagc agtcgttcac cccggccggg aacgggtgct cgggggcgag
     5761 ccggtagtcg accgacacga cgatggcgtc ggcactcacg gcgtgcaacc gggcgacggg
     5821 gtcatgggtg tccaggtcgc cgagcgagaa tccgccgcca tggtagaaga cgacgacggg
     5881 caggggtgtg tcgtcgctgg cgggccaata gatccgcacc ggaatgtcgg tgaactcgcc
     5941 gtaaccgatc gtccgttcct cggtccgcac gtccggaacc agttccgggg gcaccttgag
     6001 cagccgcagc ctcgagcgcg cgacctcgac accgtcggcg gcgttgaagg tcatcgggaa
     6061 cgcgtcgagc aacattttga acgtcggatc gatgcccggt ggggcgaggg ccggctcaat
     6121 catgcgttca ccgtacgctt ccctcccgtt tgtgcagcgt atcccccagc gccgcgagtc
     6181 cgcgctccag ctcggcggtg gcggcgggga ccactcctgc gtagccgagg tagccgtgca
     6241 ccatcgtctc ggcgttgtgc acctcgacgg gcacgccggc cgcggccagc aactcgccgt
     6301 aacggatgcc gtcgtcgcgc agcgggtcgt agccggcgac cgcgatgtag gccggtgcca
     6361 aatcgcccag attctcggcg cgccccggcg ccatccccgg cggcgggtcg gacaagtcga
     6421 cttcgcctgc gtaccaaagg gagagcgcgg tgacggcttt gacgtcgagg atcggcgcgt
     6481 cggcgttttc ggtgaaggac ggcagcgacg ggtcccacat cgtcgacgga taccacaaca
     6541 gctggaacac gatcggcggt ccgccgccat cacgcgcgcg ctgcgccatc accgccgcga
     6601 tggttccgcc ggccgaatcc ccggcgacgg ccatccgggt cgtgtcggca ccgatgtcgg
     6661 ggccgtgcgc ggcgacccag agtgttgcgg cccaagcgtc ttcgacggcg gcggggtagg
     6721 ggtgctcggg cgccaaccgg tagtcgacgg cgacgacgat cgcgtcggcg cccgccgcat
     6781 gctggcgggc ggagccgtcg tgggtttcga ggtcgcccac gacgaagccg ccaccgtgga
     6841 aatacagcac gacgggtgcc ggcgcgccgc ccgcggaata agttgacggc caatagatcc
     6901 ggatgccgat ggggcccgcc ggacccggaa tggcccggtc ctcgacgcgc acctcgggat
     6961 gcagcggaag acgcggcagg tcgcggaacc gttgccgcgc cacggcgaca ccgtcatcgg
     7021 tcgttagccg gaacggaacc gcatccagta ccttcagcag aatggggtcg ataccgggtt
     7081 ttccgtccgc tgtgctgtcc aggctgggca tggaagtacc gtacgcacct cggatggtgt
     7141 ccggtgcctg ggtggtcgcc ggctgggcgg gcgtcgggta cggcgtctac ctgacggtgc
     7201 tcgcgttgcg caccccgccg gggtcggcgt tgaccgggca ctgggttatg cagccgccgt
     7261 tcaaggcatt gatggcgttg ttgctgaccg tcgccgcggt ggcgcatccc atcgtccgcg
     7321 aacggcgctg gctgatgccg gcgctgctgc tgtcggccct cggcgactgg gtgctggcga
     7381 tcccgtggtg gacactgtcg ttcgtcgtgg ggctggtgtc attcctgttg gcgcacttgt
     7441 gtttcctggc cgcgctgttt ccgctggccc gcgcgtcccg ggtgcgcctg gccgccgtcg
     7501 tgttgatgtg cctggcgacg gtgtcgttgc tggcatggtt ctggcctcac ctgggccgcg
     7561 accagctgac gattccggtg acgctctaca tggcggtgct gaccgcgatg gtctgcgcgg
     7621 cgctcttggc agacctgccg acgatctggg ccgcggtggg ggcggtgtgt tttgcggcgt
     7681 cggacgcgat gatcggcatc gaccgcttca tcttgtacaa cgacgcactg gccgtgccga
     7741 tctggtggtt gtacgccgcg gcccaaatcc tcatcacggc cggattcttc ttcggtcgac
     7801 aggaacgcga cgagccagag ctggagccat tcgcaggcta gttgtcggtc cccggcgata
     7861 cggtgtgcct cggatggtta atgatgttat ttagcaacgt atctcgggca ccgtgacccg
     7921 gaacggcacc gcgcgcacgc ccgtcgaggt cgaaccgatc gcccgagtgc tgccgatgct
     7981 gtcggtgccg cacctggacc gcgagttcga ctacctggtg tcgcccgagc agtccgacga
     8041 cgctcagccg ggcgtgcggg tgcgggtgcg gttccacggc cggctggttg acgggtttgt
     8101 actcgagcgc cgcaacgaca ccgatcacgc cggcaaactg ggctggcttg atcgcgtcgt
     8161 ctcccccgaa ccggtgctca ccgcggagac ccgccgtttg gtcgacgcgg tggcggcgcg
     8221 ctacgcgggg acccggcccg acgtgttgcg cctggcggtg ccggcccggc acgccagggt
     8281 ggagcgggaa agcacggctg tcgtcccgct gcccgtcgtc gcccccgtcg acccgtcggg
     8341 ctgggagtcc tacggccgcg gcggccagtt cctggccgcg ctggccgagt cccgtgccgc
     8401 ccgcgccgtc tggcaggtgc tcccgggcga gccgtgggcg gaccgattcg ccgaggccgc
     8461 cgcgcagacc gtacggacgg gacgggccgt gttggcgatc gtgcccgatc agcgggatct
     8521 ggacgcgctg tggcgggctg cgacgacccg catcgacgag gccagcgtgg tggcgctttc
     8581 ggcgggcctc ggcccggccg cacgctaccg gcgttggctc gcggcgctgc gcggcaccgc
     8641 gcgggtggtg atcggcaccc gcagcgcggt gttcgcgccg ctgagcgact tgggtctggt
     8701 gatggtctgg tcagacgccg acgacagcct ggccgaacct cgggctccct acccgcatgc
     8761 ccgggaggtg gcgatgctgc gcgcgcatca ggcccggtgc gcggggctga tcgggggcta
     8821 cgcacggacc gctgaagccc acgcgctggt gcgcagcgga tgggcccacg acatcgtcgc
     8881 ggcccggccg gcggtgcgtg cccgcacgcc gcgggtggtg gccctcgacg acaccggcta
     8941 cgccgacgaa cgcgacccgg ccgcgcgcac cgcgcggatt ccgtccatcg cgctgcgcgc
     9001 cgcacgctca gcgctggagg ccggggcgcc ggtgctcgtt caggtcccgc ggcgcgggta
     9061 tgtcccgtcg ctggcgtgtg ggcgctgccg caccatcgcc cggtgccggc actgcaccgg
     9121 cccgctgtcc ctgcaggagc acggcgcggt gtgccgctgg tgcggccgcg tcgaaccggc
     9181 cccgcggtgc gcgcgctgcg gttcggacgc ggtgcgagcc gtggtcgtcg gggccaggcg
     9241 caccgccgaa gagctcggcc gcgccttcgc cggtacgccg gttgtcacct cgtcgggcga
     9301 ggccgtcgtc tcggaggtcg ccgcccagcc ggcgctggtg gtcgccaccc cgggtgccga
     9361 accccatgca gcgggcgggt acggcgcggc gctactgctg gactgttggg cactgctggg
     9421 ccggcaagac ctgcgggccg ccgaggacgc cctgtggcgc tggatgagcg ccgccgcact
     9481 cgtgcgggcc cgcggcgacg gcggtgtggt gacggtggtc gccgacgcgt cgatccccac
     9541 cgtgcaggcg ctgatccgct gggatccggt ggggcatgcg gaagccgaac tggtggctcg
     9601 caccgaagtc ggcctgccgc ccagtgtgca gatggcggcc atcgacggag cggcgggcgc
     9661 cgccgaggcg ttgctgggcg aggcccggct gccggacgga gccgagctgc tcggcccggt
     9721 ggacttgccg cccggcgtgc ggcgcccacc gggcacgccc accgatgtcc cggcgatcag
     9781 gatgctggtg cgggtccgtc gcgagcaggg gctccagctg gcggcggcgc tccggcgcgg
     9841 cgtgggcgtg ctcagcgccc ggcaatccca cgagccggtc cgggtccaga ttgacccgct
     9901 gcacatcggg tagccactgt gccggctctc ggcacacccg gcgccagacc atgccctcag
     9961 acgatagtat gcaatagaaa tagtcaatcc cgaagacatt agaagcctgt tacactttct
    10021 gttctttagc ccgctgatgg tccgggaacg gcccagtgaa aggggcgcga tggccacgga
    10081 caacgcgacg acggccgacg agtcgctgga tgcgatcacc gatgcgttgc tgacggcttc
    10141 ccggttgctg atggccatct cggcgcgctc aatcagccag gtcgacgaga ccattacgat
    10201 tccgcagttc cggaccttgg tgattttgtc cagccggggt ccgatcaacc tggccacgct
    10261 ggcgggtctg ctgggcgtgc aaccgtcggc caccggccgg atggtcgacc ggctcgtggc
    10321 cgccgggctg atcgaccgcc tgccgcaccc gacctctcgg cgcgaactac tggcggcact
    10381 gaccaagcgt ggccgcgatg tcgttcgtcg cgtcatcgcc taccggcgcg ccgagatcgc
    10441 cgccatcgtg gcgaagatgc cgccgacgga gcgccgcggg ctggtgcgag ccctgggggc
    10501 gttcaccgcc gccggcggcg agcccgatgt gcaactggac gtggatatcg acttgtagtc
    10561 gtccgggtcc cgtgccgggc tgggagggcg gcccggcacg ggatcgactc ccggcatgtc
    10621 ggccggaaat ccggtgcttc ttagactgtg gcggtgcgtc ttgtcttcgc cggcaccccg
    10681 gaacccgcgc tgcccgcgct gcgtcgcctc atcgagtcac cccggcacca ggtggtcgcc
    10741 gtgctcaccc gacccgacgc cgcggccggc cgccaccgca agccggagcc gtcgccggtg
    10801 gcccgcgaag cgctcgaccg tggcattccg gtgttgcggc cggcgcggcc caactcgcca
    10861 gagttcgtcg ccgagctgtc ggagttggcc ccccagtgtt gcgcggtggt ggcctacggc
    10921 gcgctgctgc gcgacgacct gctcgcggtg ccgccgcagg gctggatcaa cctgcacttc
    10981 tcgttgctgc ctgcctggcg cggtgcggcc ccggttcagg ccgcgatcgc cgcgggggac
    11041 gccatcaccg gggcgaccac ttttcagatc gagccgagcc tcgactcagg accggtgtac
    11101 ggggtcgtca ccgagacgat ccggccgacc gacaccgcgg gcgatctgct ggagcggctc
    11161 gcccattcgg gtgcggcgct gctgtcggcc acgctggacg gcgtcgccga cggaacactg
    11221 atgccgcgac cgcaaccagc cgacggggtc agcatcgcgc ccaagatcac cgtcgaacag
    11281 gcccgggtgc gctgggatct gccggcaccg gtggtggagc gtcgcatccg cgccgtcaca
    11341 cccaacccgg gggcctggac gctcatcggc gacctgcgca tcaagctcgg accggttggc
    11401 ctcgaccagc ctccaaaacc attgccgccc ggtgccattc acgtcgaccg ccacagcgtg
    11461 tggatcggca ccggcacgga accggtgcgg ctgggccaga ttcagccgcc cggaaagaaa
    11521 ttgatgaacg ccgtcgactg ggcgcgcggt gcccggctcg acaccgccgt gcgggcgtca
    11581 tgagcccgcc ccgaccatcg gctaaagggc gcggaaagcc gcgccggccg ctggacccgg
    11641 cgcgtgccgc cgcgttggac gtgctccggg ccgtccgcga gcgcgatgcg tacgcgaacc
    11701 tggccctgcc cgcgctgctg cgcgaacgcg gaatcagcgg ccgagacgcc gctttcgcca
    11761 cggaactgac ctacggcacc tgccgcaccc agggcctgct cgacgcgatc atcggcgcgg
    11821 cggccggacg ctcgccggaa gccatcgacc cggtgctgct cgacctgcta cggctgggcg
    11881 cctaccagct gctgcgcacc agggtcgacg catatgccgc ggtgtccacc acggtcgaac
    11941 aggccggaat cgaattcgac tcggcgcgag cgggtttcgt caacggcgtg ctgcggacca
    12001 tcgcggcccg cgacgagaag tcctggatgg acgagctggc tcccgacccg gtgcgggacc
    12061 cgatcgggca tgccgccttc gtgcacgccc acccgcgctg gatcgcccag gccttcgccg
    12121 acgcgctggg cccggacgcg gccgaactcg acgccgtgct ggccagcgac gatgaacgac
    12181 cccgggtgca cctggcggcg cgccctgggc tactgaccgc cgccgaattg gccgacgcgg
    12241 tgggtgggac cgtcggcacg tattcgccgt atgcggtgta tctgcccggc ggggaccccg
    12301 gacggctcgc gccggtccgc gacggcgccg cgctggttca ggacgagggc agccaattgg
    12361 tggcccgcgc gctgacgttg gcaccggtcg agggcgacac cggccggtgg ctggacctgt
    12421 gcgccggacc cggcggcaag acggcgctcc tggccgcgct cggcgtggcg tccggcgctc
    12481 gggtcacggc ggtggagccg gcgccgcggc gcgccgacct ggtggacgag aacacccgc
//
LOCUS       DASSGQ010000023        12316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1156121,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000023 DASSGQ010000000
VERSION     DASSGQ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12316
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156121"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            416..1114
                     /locus_tag="VFI55_00995"
     CDS             416..1114
                     /locus_tag="VFI55_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007529415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7072646.1"
                     /translation="MTIELRDVVREYRVGGQTVRALDAISLRLEGGQFVSMVGPSGAG
                     KSTLLHLLGALDSPDSGSIEFDSDEVGRLTDEQQSQFRHHRVGFIFQFFNLLPTLSAW
                     ENVAIPKLLDGVRLGRAKPDAIRLLERVGLGNRTEHRPAELSGGQMQRVAVARALMMD
                     PPLILADEPTGNLDSTTGSSILKLLGEVAHEPGRGRLVVMVTHNSEAADATDRIIMLQ
                     DGRVVSDERSAVLV"
     gene            1111..3627
                     /locus_tag="VFI55_01000"
     CDS             1111..3627
                     /locus_tag="VFI55_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7072647.1"
                     /translation="MRPTSALSAAASRLRVFSLRELAVHRRRTIASIAVMAVSAMYLV
                     AVFGIFGSITGSVNRLADGIAGIASLEVSGITDAGFPDTITADVAAVPGVATAAPMIR
                     SSASSSSGPVLLFGVDERTAALGGALKDVLKDGTAAQLAGPPGPANGVRVGPGVGRAK
                     GATFQLGSASVTVTEVLQGKQVADLNDGHYVLAPLALAQSITGRQGQLDSILITTKPG
                     ADAAKVRAAVSAAVNGRAIVADPSMRATRAGDGVRLMNYMALMGAMVALIVGAFLIYT
                     TMTMAITQRRPVISMLRAIGGRRVTIVRDMLVEAAILGVIGAAIGSAIGIVMGRIAIG
                     RLPPAMTQGLEARIEYWLPGYAIPAAVAVTALTSVVASAVAARQVYKVAPIEALAPVG
                     VSAADRVPRWLRMASGVGAVSVFAVSLFVVVTQRGALAFAAMSAVFAAEVALGFALTM
                     PIVKATAATARIFGSVGALAAATIERAPRRVWATVMTVLIAVVTTVSITGTNADMIRS
                     ARAIVSPVADAPVWVSVNPPDSYPTDALPQGLSKKIAALPGVRRVTEGAFGFAEVGST
                     RVMLDGFSSGTADPLYRAVDEHIRDEVLAGRGVVLSQNLGETLHVRVGGQLRMQTPHG
                     ARQTTVLALVPYFSTVIGTVGMSLDQMRSWFDRPVATTLEIAAAPGADTQRLLADVRR
                     AVPAPNYVYDGRAKLAGLEAPMRQSMFIANAVWIIVVFVAAVALLNTLTLSVLERRRE
                     IGVLRAMGSSRRFTLRMVLAEATGIGVIGGVLGLAFGCADQWLFSLVSGDMMNFPVVF
                     RPSWTAIVFTAGALALSLLGSVPPARRAARLNIIEAVGVE"
     gene            complement(4028..4534)
                     /locus_tag="VFI55_01005"
                     /pseudo
     CDS             complement(4028..4534)
                     /locus_tag="VFI55_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PPE family protein"
     gene            complement(4659..5288)
                     /locus_tag="VFI55_01010"
     CDS             complement(4659..5288)
                     /locus_tag="VFI55_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7072648.1"
                     /translation="MTRDNPLFPYVYRFGQPLFDRLFYYRYRRAALAHATGRLLMVGL
                     GPGTDLKFVPHGVTSIAAVEPVAVFRRMASRLAFRRGIAVDIVDGTGESIPFPDNTFD
                     SVHIGLVLCSVDDVAATLREIRRVLVPGGRLVVLEHVRGEGAMGRFQDLVAKPWSRLA
                     GGCEPNRRTIDAIAAAGFDITQLRSIRTPVPFPCKPHLQGVATAIANND"
     gene            complement(5285..5854)
                     /locus_tag="VFI55_01015"
     CDS             complement(5285..5854)
                     /locus_tag="VFI55_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HET7072649.1"
                     /translation="MRADFDAPSRWLWLVKWCVLAVPHYPILILLYLVYPLLTIVAGV
                     AILFTGRYPRPIFDFNVGVLRWSWRVMNYRFPMNSTDKYPPFTLASRPDYPGDLEVEY
                     PERLTNWAVLVKWWLLALPQILLCWAMEPLLQVLTVIAAVTLLAIGTIPQGMFDLLMG
                     IVRWRYRVAVYVSLMSDEYPSFQMDLGSR"
     gene            complement(5911..7104)
                     /locus_tag="VFI55_01020"
     CDS             complement(5911..7104)
                     /locus_tag="VFI55_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7072650.1"
                     /translation="MLVIGAGVSGISVARGLLRDGHNVMVFDQRNDTRAGGGAVTIWS
                     NGETVLRQLGVDMDGAGQLLSSVRVMTSRGRRLATLDVTAMVNRLGAPVRMVPRRVLL
                     ERLLEGFPGDRILCNCRASRLVTRSEGVQVEFDDGSFTEGDLVIGADGLHSMVRDFVG
                     APAARPTGWCSWQGLVSLPGLVDKRVATIVIGEHGNTGIWPAGGSDVQWWFDLPWSYD
                     FVRPHRPIDLIRSNFGGWSDAVDQVLATLTDEDLAGSPYPHFRHPIAGTSDAGAVTLL
                     GDAAHTMPPTLAQGTNQALLDTMVLCRALSELRNGPESGGDLLRALRGYEKTRRRRVK
                     AVSRVASLQVAHGESVLRPAALISDRLQTRVLTEFVRWTSHPRITAEIDRDLATATAV
                     APCPQ"
     gene            7457..8077
                     /locus_tag="VFI55_01025"
     CDS             7457..8077
                     /locus_tag="VFI55_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072651.1"
                     /translation="MRISRTVIAGVAAATALTVSVAGCGSKQTSSPSSSGSATSAKSA
                     TSSAPTSSASAQPTDYSGLLIQATDINAPVQFTGGPLTNNPNGQPGVAVTFSTQDNSH
                     VIKDTIQVLADPGAATNALNAAKGGQGNAIKNPTSDSFSVGTGGTVLSGNSPDNSKGV
                     TTVLFTEGKALVTLEFDGPVDSLPPQDFVTDVAQKQDAAVKKGLGG"
     gene            8142..8627
                     /locus_tag="VFI55_01030"
     CDS             8142..8627
                     /locus_tag="VFI55_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072652.1"
                     /translation="MPVAVPSTALAQPADYTGLLIQASDINAPEIFTASPPVNNPNGQ
                     PGVSTTYSNEGRTHVIADSIQILPDPAAALSALESAKATRDGYVHGVAEPIGIGTGGT
                     TISGPSPDGSTGVTVLLFTEGNAFAELEFDGPPDALVPPEFVTDIGQKQDAAIKKGLA
                     G"
     gene            complement(8677..9498)
                     /locus_tag="VFI55_01035"
     CDS             complement(8677..9498)
                     /locus_tag="VFI55_01035"
                     /inference="COORDINATES: protein motif:HMM:NF025700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HET7072653.1"
                     /translation="MLNGVMLLWWILTIPSLLFVVIDIWRNAPESPVLKWAFVILTLF
                     TGPFGAFFYVLGCREPIRGTHDEYVAARWRQVLGSTMHCVAGDGIGIVVAAAVGSGLA
                     LSFWPDFLFEYALGFGFGWAFFQAFAMRDMAGGSYLASLKMTFLPELLSMNLLMTGMV
                     LTSSFAMMRVAGADNPTRPQFWFVMSMALLVGFVCAYPINWWLVAHHMKHGMLTVRRE
                     REPTPMAGMAMGHTSGRTPEQSPAEQPTAGVKAAMTVASFAILGAALGTVVQFGM"
     gene            complement(9622..10098)
                     /locus_tag="VFI55_01040"
     CDS             complement(9622..10098)
                     /locus_tag="VFI55_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET7072654.1"
                     /translation="MNPLQRVARNPTAYRWLVLKGRPIALGLEALLRFATTGRIGVLD
                     LTGLPNVQVTTSGRKTGLARTATVQYVPTGEGLIVVGSNWGRERHPSWSANLKAAQRI
                     TVRERGHRFTANVRLLSGEERDRAWATVLTQWPNYQVAQDCAGGRRFRLFLLTRAP"
     gene            complement(10274..10801)
                     /locus_tag="VFI55_01045"
     CDS             complement(10274..10801)
                     /locus_tag="VFI55_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02444 family protein"
                     /protein_id="HET7072655.1"
                     /translation="MGEPGNTDFFKRFALALYRRAGVADACLDLQNRHDLDVNVVLFA
                     AFVGAVLRQPLTAADLGLASDRVNAWHQEVVRPLRAVRQRLKAGPVPAPNEATGRLRH
                     KLAQLEIEAEVIELDQLGALIPELQAPRSAGNPVECAAAAIETVIRTHTAAALSDQDR
                     KAIDRVATEAARIET"
     gene            complement(10859..11635)
                     /locus_tag="VFI55_01050"
     CDS             complement(10859..11635)
                     /locus_tag="VFI55_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7072656.1"
                     /translation="MTPTHTDALAKTTALVTGASSGIGAATAKALAAQGAAVALLARR
                     ADRLADLKADIESAGGTALVVRADVTDAEQVAGAVQRTVDELGRLDTLVNNAGLMQSG
                     PAAEAPLRDWDDMVAVNIQGVLYVTRAALPHLIDAAATSPRGVADLVTISSTAGWVAR
                     PNTAVYSLTKFGVNAFSEGVRQEVLGKRVRVGVVGPGTVDTELFTHLAAPSRDAFETQ
                     TADMVKLRPEDIADAVLYMVTRDRRVAVNHMLVRAAEQTW"
     gene            complement(11632..>12316)
                     /locus_tag="VFI55_01055"
     CDS             complement(11632..>12316)
                     /locus_tag="VFI55_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016706265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET7072657.1"
                     /translation="MRYGDSTVGAAAAYAAAVALLHRERTGAGQFIDVSAVEALSSMI
                     GDCLLEQSLTGKRLGPNGNDHPDMSPHGCYPCADGSWVAVAVADDAEWNRLCDVLGAA
                     TLAQDPRYATMDERHRHAEALDADLARFTRNHDAEQLAHRLRVAKVPANKSANAIDVI
                     GDERLWDRALYRFVTDHREGQRPVLGPSWRMARAPARIARGAPDLGEDTEYVLREILG
                     AGSPTEGRT"
BASE COUNT         2234 a         4040 c         3980 g         2062 t
ORIGIN      
        1 cgccgacacg gcgcgagtga ggagctgggc catcgaaccc agcccagcga cggcgagcgc
       61 cgacacggcg cgagtgagga gctgggccat cgaaccgagc ccagcgacgg cgagcgccga
      121 cacggcgcga gtgaggactg aatcctcttg gttgtgatcg agcttcatgg ccgttgcgca
      181 cgaaacacga cgtcgcagaa agtcgcgggg tctttctgga agcctcgccg aatccttttg
      241 aggcgcaccg ctgtctttgg atgaacggaa cgcggcgccg tctcgttata tccgatacca
      301 gcgcctgggg ggacggcgtt catgtgtggg gaagcgctga ctagtgcgga ctcgcgggag
      361 gatcatgttc agacgcgtcg acaccgacac ctccgccagt cccaagcaca aattgctgac
      421 gatcgaattg cgtgacgtgg tgcgcgaata cagagtgggc ggtcagactg tgcgggcact
      481 cgacgcaata agtctgcggc tcgagggcgg gcagttcgtg tcgatggtcg gaccgtcggg
      541 ggcgggcaag agcaccttgc tgcatctgct cggcgcactg gactcccccg attccgggtc
      601 gatcgaattc gacagcgacg aggtcggtcg cctcaccgat gagcagcagt cgcagtttcg
      661 ccaccatcgc gtggggttca tattccagtt cttcaatctg ttgccgacgt tgtcggcgtg
      721 ggagaacgtc gcgatcccga agctgctgga cggtgtccgg ctgggaaggg ccaaacccga
      781 tgcgatccgg ctgctggagc gggtggggct tggtaaccgg accgagcaca gacccgctga
      841 gctgtccggc gggcagatgc agcgagtagc tgtggcgcgc gcgctgatga tggacccgcc
      901 gctgattctg gctgacgaac caaccgggaa cctcgattcg accacgggtt cgtccatctt
      961 gaagctcctc ggcgaggtgg cgcacgagcc cggccgcggc cggctggtgg tcatggtcac
     1021 ccataactcg gaagccgcgg acgcgaccga ccggataatc atgctgcagg acggtcgggt
     1081 ggtctccgac gagcgatcag cggtcctggt gtgagaccca cttccgcgct aagtgccgct
     1141 gcgagtcggc tccgagtgtt cagcctgcgt gaactcgcgg tgcatcgccg ccgcacgatc
     1201 gcatcgatcg cggtgatggc ggtgtcggcg atgtacctcg tcgccgtatt cggcatcttc
     1261 ggatcgatca ccggatcggt caatcggctt gccgacggca tcgcgggcat cgcatcgctc
     1321 gaggtttcgg gcattaccga tgcaggattt cccgacacga taaccgccga cgtggccgca
     1381 gttcccggcg tggcgacggc agcaccgatg attcggtcgt cggcgtcctc gtcgtcgggg
     1441 ccggttctgt tgttcggcgt cgacgagcgc actgcggcct tgggcggcgc cctgaaagat
     1501 gtcttgaaag acggcacagc ggcccagttg gcgggtcccc ccgggccggc gaacggggtc
     1561 cgcgtcgggc cgggtgtcgg tcgagccaaa ggcgcgacgt tccaactcgg ctcggcatcg
     1621 gtcacggtca ccgaggtgct ccagggcaag caggtggcgg atctcaatga cggacactac
     1681 gtccttgctc cacttgcgtt ggcgcagagc attaccggac gtcaaggcca gctcgactca
     1741 atcctgatca ccactaaacc cggtgccgat gcggccaagg ttcgcgccgc ggtctccgcc
     1801 gcggtgaatg gtcgggccat cgtcgccgac ccgagtatgc gggctacccg cgccggcgac
     1861 ggtgtccggt tgatgaacta catggccctg atgggtgcga tggttgcgtt gatagtcggc
     1921 gcattcctga tctacacaac gatgaccatg gcgatcaccc agcgccggcc ggtcatctcg
     1981 atgctgcgcg cgatcggcgg ccgacgtgtc accatcgtcc gcgacatgct cgtggaagct
     2041 gcgattcttg gagtgatcgg cgcagccatc ggatctgcca tcggaatagt aatgggccgc
     2101 atcgcaattg gccgtttgcc tccggccatg acccaggggc tcgaagcccg catcgaatat
     2161 tggcttcccg gttacgcgat accggccgcc gtcgcggtga ccgcgctgac aagtgtcgtt
     2221 gcgtcggcgg tggcggcgcg gcaggtgtac aaggttgcac cgatcgaagc gctggcaccc
     2281 gttggggttt cagcggcgga ccgggtgccg cgctggctcc ggatggcatc cggggtcgga
     2341 gccgtgagcg tcttcgcggt gtcgctcttc gtggttgtca cccaacgggg cgcattggcc
     2401 tttgccgcga tgtcggcggt gtttgctgcc gaggtagccc ttggcttcgc cctgaccatg
     2461 cccatcgtca aagccaccgc cgcgactgcc cgaatattcg gctccgtcgg ggcccttgct
     2521 gcggcgacca tcgaacgcgc gccgcgacgg gtgtgggcga cggtgatgac ggtgctcatc
     2581 gcggtggtca ccaccgtctc gatcaccggg acaaacgccg acatgatccg gtcggcgagg
     2641 gccatcgtct ccccggtcgc cgatgccccc gtgtgggtga gcgtaaatcc ccccgacagc
     2701 tatccgactg atgctctccc gcaaggtctt tccaaaaaga ttgccgcatt gccgggcgtg
     2761 cgacgcgtga ccgagggtgc ctttgggttc gccgaggtgg gcagcacgcg cgtcatgctc
     2821 gacgggtttt cctccgggac agccgatccg ctgtaccggg cggtcgacga acacattcga
     2881 gacgaagtcc tggccggtcg gggcgtagtg ctctcccaga atctgggcga gaccctgcac
     2941 gttcgtgtcg gcggccagtt gcggatgcaa acgcctcacg gcgcgcggca gacgaccgtg
     3001 ctggccctgg tgccgtactt ctcgacggtg atcggcacgg tcgggatgag cctcgatcaa
     3061 atgcgctcct ggttcgaccg cccggtagcg accacgctcg agatcgccgc ggccccaggc
     3121 gcggacaccc aacgcttgtt ggccgacgtc cgtcgggcgg tgccggcgcc gaactacgtc
     3181 tacgacggcc gggccaaact ggccggcctc gaggcgccga tgcgtcagag catgttcatc
     3241 gcgaacgccg tatggatcat cgtcgtgttc gtcgccgccg tcgcgttgct caacacgttg
     3301 acgctttcgg tgctggaacg gcgccgcgaa atcggcgtgc tacgagcgat gggatccagt
     3361 cgccggttta cgttgcggat ggtgctcgcc gaagcgactg gcatcggggt catcggcggt
     3421 gtgttgggtc tggcgttcgg atgtgcagac cagtggctgt tcagtctggt cagcggggac
     3481 atgatgaatt tcccggtcgt cttccggccc agctggacgg cgatcgtctt caccgccggc
     3541 gccttggcgc tcagcctgct cggctcggtg ccgcccgcca ggcgcgcggc acggctgaac
     3601 atcatcgagg ccgtcggcgt cgaatagctc ctcgcgtgcc cgtcaggcct cagcccgccg
     3661 gccgtcagcg tcagaagccg agcgggagaa gcgcggtaaa cagcggcgcc aagggatccg
     3721 tgaccggaag cgcgatcgaa ctattcaaga acggggcgac cggactttgg aacgtgagta
     3781 tggtctgacc gggtgccccg aggcccaagg tggtttcagc cccggaagtc accccagccg
     3841 tgaagagcgg ggtcgagatg ttagtcccaa caaagtgatt gtcgtccggg tcggcaattg
     3901 aggtgttcga gaacaaaccc ccgaaaccgc tggtttggct catgaagtcc gggtcgccgg
     3961 ctacggggtt ggtgaccggg ctgaggccca gcgccccggc gagccgcgtc gccagcccgt
     4021 tcagcgactg caacggcgcc agctgcgtgg cggcggccga ggccccggcg tgatagccgc
     4081 ccatcgcggc cacatcctgg gcccacatcc gctcgtagtc ggcctcaaca gccgcaatcg
     4141 ccggcgcatt ttgcccaaag aaattcgaca tcacgagctg caccaaccca ttccggttgg
     4201 ccgccaccgc caacggatgc accgtggcag cccgggcagc ctcaaacgcg ccggccaccg
     4261 cccgagcctg ccctgccgcc ccggtcgccc gagtcgccgc cgcatttagc cagctcacat
     4321 agggcgccgc cgcagccatc atcgccgccg acgccggacc ctgccacgcc tggcccgtca
     4381 gacctgacgt caccgacgaa aacgacgacg cggcggcccc caactcggag gccagcccat
     4441 cccaggccgc cgccgcattc aacatcggtt ccgaacccgc gccagagaac atccgcgccg
     4501 agttgatctc cggcgccaac accgtaaagt tcatcgccac agtcccttcc gtaacggccc
     4561 gtcgcgatca tgctggctgc gtgatcaccg acgacacgcc ccgtgtccca ctacacgagc
     4621 tccgcgcctt aaaggttcag tgtgatctag ctcacaattc aatcgttgtt cgcgatcgca
     4681 gtcgcgacgc cttgcaaatg gggtttgcac gggaacggca caggggttcg gatactgcgc
     4741 aattgcgtga tatcgaagcc ggccgcggca atggcgtcga tggtccttcg gttcggctcg
     4801 cagcccccgg ccaagcggga ccacggcttt gcgaccaggt cctggaaacg gcccatggcg
     4861 ccttctcccc ggacgtgctc gaggacgacc aaccttccgc ctggcaccag cacccgtcgg
     4921 atctcgcgca gcgtcgcggc cacatcgtcc accgagcaca gcaccaaccc gatatgcacc
     4981 gaatcgaagg tattgtccgg gaacggaatc gactccccag tcccatcgac aatgtcgacg
     5041 gcaatgccgc ggcgaaaggc aagacgggac gccatccgcc ggaaaacggc caccggctcc
     5101 acggcggcga tcgatgtcac cccgtgcggc acaaatttca aatcggtccc cggcccgaga
     5161 ccgaccatca gcagccggcc agtcgcatgt gcgagcgccg ctcgacgata tcggtagtag
     5221 aagagccggt cgaagagggg ctgtccgaac cgatatacgt agggaaacaa tggattatcg
     5281 cgcgttatcg gctacctaga tccatctgga acgacggata ttcgtcgctc atcaacgaaa
     5341 catacaccgc cacccggtat cgccatcgaa cgatacccat cagcaggtcg aacatgccct
     5401 gggggatcgt tccgatggcc aaaagcgtca ctgccgcgat gacggtcagc acctgcagca
     5461 ggggctccat cgcccagcag aggagtatct gcggcagcgc cagcagccac cacttcacca
     5521 gcactgccca gttagtgagc cgttctggat actcgacctc cagatcaccg gggtaatcgg
     5581 gccgagatgc gagcgtgaac ggcgggtact tgtcggtgct gttcatcgga aaccggtaat
     5641 tcatgacgcg ccacgaccaa cgaagcaccc cgacgttgaa gtcgaagatc gggcgcgggt
     5701 agcgcccggt gaacaggatc gccacgccgg caacgatcgt cagtagcgga tacaccagat
     5761 agagaagtat caatatggga tagtgcggaa cggccagtac gcaccacttg actagccaga
     5821 gccaccgcga gggcgcgtcg aaatcagccc gtatgcgcac ggtatcgacc gaagtgtgcg
     5881 ccgatcgggg cgatgtggtt ctggcggcgt tcattgcgga cacggggcaa cagccgttgc
     5941 ggtcgcaagg tcgcggtcga tctccgcggt gatccgggga tgactggtcc aacgcacaaa
     6001 ctccgtcagc acccgtgtct gcaagcggtc cgagatcaac gccgccggtc taagcactga
     6061 ttcaccgtgg gctacctgaa gtgatgccac tcgggacacg gctttgaccc ggcgtcgcct
     6121 ggtcttctcg tacccgcgca gcgccctcag aaggtcaccc ccggactccg gcccattccg
     6181 cagttccgaa agcgccctgc acaacaccat ggtgtccagc agcgcctggt tggttccttg
     6241 cgcaagggtg ggcggcatcg tgtgcgcggc gtcgccgagc aaggtcaccg cgcccgcgtc
     6301 cgacgtcccg gcaatcggat gccgaaagtg ggggtagggc gaacccgcca ggtcttcgtc
     6361 ggtcagcgtc gcgagcactt ggtccaccgc gtcagaccag ccgccgaagt tggatcggat
     6421 cagatcgatt ggacggtgcg gcctgacgaa gtcgtacgac cacggcaggt cgaaccacca
     6481 ctgcacatcg gacccgcccg ccggccagat accggtattt ccgtgctcac cgatgacgat
     6541 cgtcgcgacg cgcttgtcga cgaggccggg aagggagacc agtccctgcc agctgcacca
     6601 gcccgtcgga cgcgcggccg gcgcgccgac gaaatctcga accatcgaat gcagaccatc
     6661 ggcaccgatc accaagtcgc cttcggtgaa actgccatcg tcgaattcga cctgcacgcc
     6721 ttcggaccgg gtgacgagcc tcgacgctcg gcagttgcac aggatgcggt cgcccggaaa
     6781 gccctcgagc agccgttcca gcaggacacg acgtggcacc atccggacgg gcgcacccag
     6841 ccggttcacc atcgcggtca cgtccaaagt tgcgagccgt cggccccgag acgtcatcac
     6901 ccgcacgctg gaaagcagtt ggccggcccc atccatgtcg acgcccagtt gccgcaaaac
     6961 tgtctcgccg ttggaccaga tggtcaccgc gccgccaccc gcgcgcgtgt cattgcgctg
     7021 atcgaagacc atgacgttat ggccatctcg cagcaatccc cgggcaacgg aaataccgct
     7081 cacaccggcg ccgatgacga gtatccgaag cggtcgcgtc cacccgcggg cgtcaccgcg
     7141 attcgggtcg gcgcgccagc tggtcacttt cttggatacg taagcgcgtg ccaatcggtt
     7201 cacgtaagcg gggagctcgg ctgccggacc tgaccaggct gcgcttgtcg cgattcgcag
     7261 gctcatccgg tgtgcggcgt gggccgatcg agggcgccga cgaatcctgc ggcaatgaag
     7321 agcgctgcct gggctagcaa cacgggtagg ccgttcatca ccgccaggac ggtcggcgag
     7381 acgaagcgat gtactgtgcg attcgggaac gaagtcgaca gtgtcgccaa aagtggcctg
     7441 gaaggagcac gatgcgatga ggatttctcg gacggtgatc gcgggcgtag cggcggcgac
     7501 agcgctgacg gtctcggtag ccggctgcgg cagcaagcag acgtcgtcgc cgtcaagctc
     7561 tggatcagcc acctcggcga agagtgcgac cagctcagcg ccgacttcct cggcgtcggc
     7621 acagccgacc gattacagcg ggctcctgat ccaggcgacc gacatcaacg caccggtcca
     7681 gttcacgggc ggcccgctga caaacaatcc gaatggccaa ccgggcgttg ccgtgacgtt
     7741 cagcacccag gacaacagcc acgtcatcaa agacaccatc caggtcctcg cggaccccgg
     7801 cgctgcaacg aacgcgctga acgcggcaaa gggcggacaa gggaacgcga tcaaaaaccc
     7861 gacatcggat tcgttcagcg tcggtactgg cggaactgtc ctgtccggga actcaccgga
     7921 caactccaag ggagtgacga cagtgctgtt taccgagggc aaggcgttag tcacgctgga
     7981 attcgatggc ccggtcgact cgctcccgcc gcaggacttt gtcaccgacg tggcacaaaa
     8041 acaagacgcg gcggtaaaga aggggctcgg tggctgagcc acggggcgcg tgtgccatga
     8101 gggtttgtcg tgcgctgctc accgcgacag cggtggcact gatgccggtg gcggtgccat
     8161 cgaccgcgct cgcgcagccg gccgactaca ccggattgtt gatccaggcg agcgatatca
     8221 acgcgcccga aatcttcacg gctagtccac cggtcaacaa tccgaacggc cagccaggcg
     8281 tcagcaccac ctacagcaac gaaggccgca cgcacgtcat cgccgacagc atccagatcc
     8341 tgccggatcc tgccgccgcc ctcagcgcgc tggaatcggc taaggcgact cgggatggtt
     8401 atgtccacgg ggtggcagaa ccgatcggca tcggcaccgg cggcacgacg atctcggggc
     8461 cttcgcccga cggctccacg ggcgtgaccg tgctgctgtt caccgagggc aacgccttcg
     8521 ccgaacttga gttcgacggt ccgcccgatg cgctcgtgcc gccggaattc gtaacggaca
     8581 tcggccaaaa gcaagatgct gcgatcaaaa aaggactcgc cggctaggcg cgccggactg
     8641 ctcggcggag cggatagccg ttagccaccg ccgatatcac atgccgaatt ggacgaccgt
     8701 gcccagcgcc gcaccaagga tggcgaaact ggcgacggtc atagcggctt tcacgcccgc
     8761 ggtgggctgc tcggccggcg attgttcggg tgtgcgtccc gaggtatgac ccatcgccat
     8821 ccccgccatc ggcgtcggct cacgttctcg acgaacggtg agcatcccat gtttcatgtg
     8881 gtgggcaacc aaccaccaat tgatgggata cgcgcacacg aatccgacga gcagcgccat
     8941 cgacatcacg aaccagaact gcgggcgagt cgggttgtca gcccccgcca cgcgcatcat
     9001 tgcaaagctg gaagtgagca ccattccggt catcagcaaa ttcatcgaca acagttctgg
     9061 cagaaaagtc atcttcaacg acgccaggta agacccgccg gccatgtcgc gcatcgcgaa
     9121 cgcctggaag aaagcccatc caaacccgaa acccagcgcg tactcgaaca ggaaatccgg
     9181 ccagaaagac agggcaagcc ccgacccgac ggcggccgca acgacgatgc cgatcccgtc
     9241 accggcgacg caatgcatgg tcgacccgag cacttggcgc cagcgcgccg caacgtactc
     9301 gtcgtgcgtt ccccgaattg gttcccggca gccgagcacg tagaagaatg cgccgaacgg
     9361 tccggtgaac agggtgagaa tcacgaacgc ccacttcagg actggcgatt cgggagcgtt
     9421 tcgccagatg tcgatgacaa cgaaaagaag cgacggaatc gtcaggatcc accacagcag
     9481 catgaccccg ttgagcatga cgaacagcct aaggcggcac cgagcccgac ggcatcgcca
     9541 ttatcgcccc gaattttcac atcccgtgcg gcgacagact ttccaaacgc agccgcgacg
     9601 gcccagcacc ccggagcgat ctcacggggc gcgcgtgaga aggaagagac ggaaccggcg
     9661 tccgcctgcg caatcctggg cgacctggta gttcggccac tgcgtcaaga ccgtggccca
     9721 tgccctgtcg cgttcctcgc ctgacagcag ccgcacgttg gcagtgaagc ggtggccgcg
     9781 ttcgcgaacg gtgatccgtt gtgccgcttt aagattcgcc gaccacgacg ggtgacgctc
     9841 ccgtccccaa ttggagccaa ccacgataag cccctccccg gtgggcacgt actgcaccgt
     9901 cgccgtgcgg gcgaggcccg tctttcgccc cgaagtggtg acctgcacgt tgggcaggcc
     9961 ggtaaggtcc agaacaccga tgcgaccggt ggtggcgaac cggagcagag cttcgagccc
    10021 cagagcgatc ggacgccctt tgaggacgag ccagcggtat gccgtcgggt ttcgggccac
    10081 ccgctgcagc ggattcacgc gaacactcta ttccctacgt gtgcggccag aacgtccgga
    10141 cgatgaaatc gtcctgacta gctaggattc ccaatgcggc ggctccagca cgggtttcga
    10201 tcattccgcg ccgtttttcg ggtcggtcct gcgcgccgcg acaccgttcg catcaccgcc
    10261 gacggcgcct ccttcacgtc tcgattctcg ccgcctcggt ggcgaccctg tcgatcgctt
    10321 tgcggtcctg atcgctgagt gcagcagcgg tgtgtgtcct gatgacggtt tcgattgccg
    10381 ctgcggcgca ttcgacgggg ttgcccgcgg agcggggggc ttgcaattcc gggatcagcg
    10441 cgccgagctg gtctagttcg atcacctcgg cttcgatttc gagctgggcg agtttgtggc
    10501 gcaaacgccc cgtggcttcg ttgggtgcgg gcaccggccc ggccttgagg cgctgacgga
    10561 ccgcgcgcag cgggcggacc acttcctgat gccacgcgtt gacgcggtca gacgcgaggc
    10621 cgagatcagc ggcggtcagc ggttgccgca gaacggcgcc gacaaacgcg gcgaacaaca
    10681 ccacgttgac gtccagatcg tgtcggtttt gcaggtccag gcaggcgtcg gcgacaccgg
    10741 cgcggcgata gagcgccagc gcgaatcgct tgaagaaatc ggtgttgcca ggctcgccca
    10801 tcgcttcttt ctccggcacc cctttgactg aggcgacgcg ttcggattcg tcggggtgtt
    10861 accaggtctg ttcggcggcg cgcaccagca tgtggtttac cgcgacccgc cggtcacggg
    10921 tcaccatgta cagcacggcg tcagcgatgt cttcaggccg gagcttgacc atgtcggccg
    10981 tttgcgtttc gaacgcgtcc cgcgacggcg cggccagatg ggtgaacagt tcggtgtcga
    11041 cggtcccggg acccaccacc ccaacacgga cccgcttgcc gagaacctct tgtcggacac
    11101 cctcgctgaa ggcgttgaca ccgaacttgg tcaacgagta gaccgcggtg ttaggtcggg
    11161 ccacccaacc ggcggtggaa ctgatggtga ccagatcggc cacaccgcgc ggcgaggttg
    11221 cggccgcatc gatcaggtgc ggaagcgccg cgcgggtgac gtagaggacg ccttggatgt
    11281 tgaccgcgac catgtcatcc cagtcccgca aaggcgcctc ggccgcgggt ccggactgca
    11341 tgaggccggc gttgttcacc agggtgtcga gacgtcccaa ctcgtcgacg gtgcgctgca
    11401 ccgcgccggc gacctgctcg gcgtcggtga cgtcggcgcg cacgacaagt gctgtgccgc
    11461 ctgcggattc gatgtcggct ttcaggtcgg ccagccgatc ggctcggcgt gccagtagcg
    11521 ccaccgcggc gccttgcgcg gccagcgctt tcgccgtcgc cgcgccgatg ccgctgctgg
    11581 cacccgtcac cagggctgtg gtttttgcca aggcatctgt gtgcgtcggc gtcatgtcct
    11641 gccttccgtc ggtgatcccg cgcccaggat ctcgcgcaga acatactcgg tgtcctcgcc
    11701 caggtcgggt gcaccccgcg cgatccgtgc cggggcgcgc gccatccgcc acgacggtcc
    11761 cagtaccgga cgctggcctt cacggtgatc ggtgacgaag cgatacagcg cccggtccca
    11821 caatcgttca tcaccgatca cgtcaattgc gttggcgctc ttgttcgccg gcactttcgc
    11881 cacccgcagg cggtgcgcga gctgctcggc atcgtgattt cgggtgaagc gcgccagatc
    11941 ggcgtcgagc gcctcggcgt gacggtgtcg ctcgtccatc gtcgcgtagc gcgggtcttg
    12001 ggccagcgtg gcggcgccga gcacgtcaca caatcgattc cattcggcat cgtcggcgac
    12061 ggccacggcg acccaggaac cgtccgcgca ggggtagcag ccatgcgggg acatgtcggg
    12121 gtggtcattg ccgttgggcc ccagacgttt cccggtgagg ctctgttcga gcaggcaatc
    12181 cccgatcatc gaggagagcg cttccacggc cgacacgtcg atgaattgcc ccgcgccggt
    12241 gcgttcgcga tgcaacagcg ccaccgcggc ggcgtaggcg gcggcagcgc caacggtgga
    12301 atccccgtaa cgcatg
//
LOCUS       DASSGQ010000024         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_913151,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000024 DASSGQ010000000
VERSION     DASSGQ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913151"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFI55_01060"
     CDS             <1..190
                     /locus_tag="VFI55_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072658.1"
                     /translation="LKASWHWPDEENRPSKYRCFTQDLAFPVETEGEYTIGAYYDVNG
                     RIEMATPIPISIALAEER"
     gene            complement(187..1419)
                     /locus_tag="VFI55_01065"
     CDS             complement(187..1419)
                     /locus_tag="VFI55_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7072659.1"
                     /translation="MTTAPTQAESQGLLLQLLDPANRADPYRLCAQFRDLGPIRLPEA
                     NLAVFSAYRDCDEVLRHPSSSSDNMHSTVAKRQIESGLVPPRQVPPSFLFLDPPDHTR
                     LRKLVSKAFAPKVVNALKPDIAALVDELLDAIAEKGQFDVVEDFAHPLPVAVICRLLG
                     VPLDDESRFSHASALLAQALDPFSTITGLPPEVMNERMQAAMWLREYFHGLIDRRRSQ
                     PGDDLLSGLIAVEESGDQLTELEIVSTCILLLIAGHETTVNLIGNAMLAMLSHPEQWA
                     ALGANTDRAPAVIEETLRYDPPVQLVGRVAAADMTIGDIEVPTGDVMLLLIAAAQRDP
                     AEFDWPDAFDPDRGTLRHLGFGRGAHYCLGAPLARLEAVVALSAVTARIPDARLYGEP
                     RYKPNVTLRGLSELTVTV"
     gene            complement(1452..>2012)
                     /locus_tag="VFI55_01070"
     CDS             complement(1452..>2012)
                     /locus_tag="VFI55_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7072660.1"
                     /translation="RHVTVVGQDWGGLIGLRLAAEHPERFARVVVANTGLPNGDQPMA
                     EVWWRFREAITTAPQLNIGAFVQSGCRQPLSEDVRASYDAPFPADEYCAGPRAMPGLV
                     PTSPDDPAAAANKAAWAKLSVSPMPMLVAFSDSDPITGPMGLIFRREMRGAQGIDHPT
                     IRGAGHFLQEDAGEELANHTVEFLRR"
BASE COUNT          371 a          666 c          647 g          328 t
ORIGIN      
        1 cctcaaagcg agttggcatt ggccggacga ggaaaacaga ccgtccaaat accggtgctt
       61 tacccaagac ctcgcgttcc cggtcgagac cgaaggtgag tacacgatcg gcgcctacta
      121 cgacgtgaac ggcagaatcg agatggccac gcccataccg atttcgattg ccttagccga
      181 agagcgttag acggtaaccg tcagctccga cagaccgcgc agcgtgacgt tgggcttgta
      241 ccgcggctca ccgtaaagcc gcgcgtccgg gatccgtgca gtcaccgccg acagagccac
      301 aaccgcttcc agtcgcgcca gaggcgcgcc caggcaataa tgggcgccac ggccaaatcc
      361 taagtgccgc aacgttccac gatcgggatc gaatgcatcg ggccaatcaa actcggcggg
      421 atcacgctgc gccgcggcga tcagcaacag catcacgtcg cccgtcggca cctcgatgtc
      481 gccaatcgtc atgtcggcgg cggcaactcg gccgaccagt tgcacgggcg ggtcgtaacg
      541 cagggtctcc tcgatcactg cgggcgcacg atcagtgttg gcgcccagcg ccgcccactg
      601 ttcggggtgg ctcaacatcg ccagcatcgc attgccgatc aggtttacgg tggtctcgtg
      661 gccggcgatc aacagcagga tgcaggtgga gacgatctcc agttcggtca gctgatcgcc
      721 ggactcttcc accgcgatca ggcccgagag caagtcgtcg ccgggttgcg atcggcgccg
      781 gtcgatcaga ccgtggaaat attcgcgcaa ccacattgcg gcctgcatcc gctcgttcat
      841 cacctccggc ggtagaccag tgatggtcga gaacgggtcc agcgcctgag ccaacaacgc
      901 cgacgcgtgg ctgaaccgcg attcgtcgtc gagagggaca cccagcaacc ggcagatcac
      961 cgcgacaggc agcggatggg cgaagtcctc gaccacatcg aattggcctt tctcggcgat
     1021 cgcgtcaagc aattcgtcca ccaacgcggc gatgtcgggt ttcaacgcat tgaccacttt
     1081 cggcgcgaac gccttgctga ccagcttgcg cagccgggtg tgatcgggtg gatcgagaaa
     1141 caagaaactc ggcggcacct gccggggcgg caccaaaccg gactcgatct gccgcttggc
     1201 gaccgtggag tgcatgttgt cgctactcga cgatgggtgc cgcaacacct cgtcgcagtc
     1261 ccggtaagcc gagaagacgg ccaggtttgc ctcgggcagc cggatcggcc cgaggtcgcg
     1321 gaactgtgcg cagagccgat acgggtcggc gcggttggcc gggtcgagca gctgcaacag
     1381 cagcccttgc gattcggcct gggtgggcgc ggtcgtcatc ctcacattct gccctcgccg
     1441 ccggaccggg ttcagcggcg gagaaactcg acggtgtggt tggccagttc ttcgccggcg
     1501 tcctcctgca ggaagtgtcc ggcgccgcgg atcgtcgggt ggtcgatgcc ctgcgcgcca
     1561 cgcatctcgc gccgaaagat cagacccatc ggcccggtga tgggatcgct gtcgctgaag
     1621 gcgaccagca tgggcatcgg actcacggac agcttcgccc acgccgcttt gttcgccgcc
     1681 gccgcgggat catccggtga ggtcggcacc agaccgggca tggcgcgcgg cccggcgcag
     1741 tactcgtccg cgggaaacgg cgcgtcataa ctcgcgcgca cgtcttcgct cagcggctgg
     1801 cgacagccgc tctgcacaaa cgcgccgatg ttcagctggg gcgccgtcgt gatggcctca
     1861 cggaagcgcc accacacctc ggccatcggc tgatctccgt tgggcaggcc ggtgttggcg
     1921 acgacaacgc gggcaaaccg ttcgggatgc tcggcggcca gccgtaaccc gatcagtccg
     1981 ccccagtcct ggccgaccac cgtcacgtgg cg
//
LOCUS       DASSGQ010000025         3336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1277992,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000025 DASSGQ010000000
VERSION     DASSGQ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277992"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            648..2498
                     /locus_tag="VFI55_01075"
     CDS             648..2498
                     /locus_tag="VFI55_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019929315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET7072661.1"
                     /translation="MTDRTGTQTLSPDGAPKLGANRGLGLDQRAEAHKLTTLGGLAAL
                     SLDALSSVAYGPESIVLVLVAAGAAAIAWTLPIAVAITTLLLVLVISYRQVIAVHPDG
                     GGAYAVAKKDLNRPVSLLAAASLIVDYVLTVAVSLAAGAASLASVFPSLAPHLLAVTL
                     VALAVITIVNMIGIAESAKVLMIPTLVFIFGILATIFVGYFRPHPVALIGSDLGPIHA
                     TEALGVVLILKAFSAGCSALTGVEAIANAVPAFRAPAIKRAQNCEVALGVLLGVMLVG
                     LAGLIRLHNMVPRGDVTILAQLTAAAFGTGWPFYVTNLAVVLVLGCAANTSFGGLPVL
                     LQLLAKDNRMPHLFMLRSERPVYRYGVAALAILAFLLLLIVGADTQKLLPVYAVGVFI
                     GFTISQTGLVKHWRQERGKGWALKALLNGSGAALTAVASVVLFATKFTEGAWLLLIIV
                     PSLMLLFDRNERYYRRVGEELGIGRTPIKPSPGADPQALVIVPIVAVSVLAQRVLQAA
                     MRLGGEILPVAVDVDPEDTQRLTEQWKQWDPGVDLRVLPSPNRSVVLPITTLVRSQLL
                     HGRHVTVLLGQVEPSRRRYRILHNQRGLVLAAALRMRTDAVVATLLVRVT"
     gene            complement(2547..3221)
                     /locus_tag="VFI55_01080"
     CDS             complement(2547..3221)
                     /locus_tag="VFI55_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HET7072662.1"
                     /translation="MPLRKWAGVGLAAAVVAATLALTGAVLPSGTLVPVAAASCPDVQ
                     VVFARGRYESPGPGVLGNAFISALESKVSKNIGVYAVRYPADTEVDVGANDMSHHIQD
                     MVNNCPDTRLVVGGYSLGAAVTDVVLAAPIGAFGFDSPLPPGTDQHIAAVALFGNGSQ
                     WVGPITNFNPTYNDRTIELCHGADPVCNPADPNTWKQNWPQHLASAYIDSGMANQAAD
                     FVAGKL"
BASE COUNT          539 a         1101 c         1098 g          598 t
ORIGIN      
        1 ccgcggcggc gggggctgtg gctaaccgcc ttgcgcggca aggtaatacc tcgggctgcg
       61 caatgacgag tacgtcggca taattctccg ggtgccgcag acacgcagag cgaaggcgct
      121 cgccaaccca gcccagcccc atggtgcgct tattcacgaa ttggagcgct acattgaggc
      181 ccaccatccc aacgtgagcg atgtcaacgt ggtgagcgcc acggacaccg gtcaggccga
      241 cacgagccat aagcccgtgc gccactggta ccacgtcacc tacgaatcat gagggtgaca
      301 cccgcggcga agtaggccgt gccgccggtg agcacatcag ggtcgcgacg gcatttgttg
      361 cagcttccaa tttgcgctcg ggaatcaagt gcgtccgaac catgacgcct accaggccgt
      421 cgccgacaat ccatacagcg gcccgctgtc gacgccgctg agcaggccgt tcttgccggg
      481 tcaggtgggt aaggcgtcag aagcgtgagt agacaccccc aacgtcgaat tgcaggagtc
      541 catatgagcg catccgtgcg ccaccttccc gattgcggcg cccccgcacc gatcgcggtt
      601 ccgcaactca gccaaaaagt gccggccccg cgcgccgagg cgtcgcgatg accgaccgga
      661 cggggaccca aaccctatcg cccgacggcg ctcccaagct cggtgccaat cgcgggctgg
      721 ggctggatca gcgtgctgag gctcataagt tgacgacgtt gggcgggctt gccgccctgt
      781 cgctggacgc gttgtcaagc gtggcatacg gcccggaatc tatcgtgctc gttctggtgg
      841 ccgccggcgc cgcggcgatc gcgtggacgc tgcccatcgc ggtggcgatc acgacgctgc
      901 tgctcgtctt ggtgatctcc taccgccagg tgatcgcggt gcatccagac ggcggtggcg
      961 cctatgcggt ggcgaaaaag gacctgaacc gcccggtgag cttgttggcc gccgccagtc
     1021 tgatcgtcga ctacgtgttg accgtcgcgg tcagccttgc cgccggcgcg gcgagcctgg
     1081 ccagcgtctt cccgtcgctc gcgccgcacc tgctcgccgt gacgctggtg gcgcttgccg
     1141 tgatcacgat cgtgaacatg atcgggatcg ccgaatcggc gaaggtgctg atgattccga
     1201 cgttggtgtt catcttcggc atcctcgcga ccattttcgt cggatacttc cgcccccacc
     1261 cggtggccct gatcggttcc gatctcggcc cgatccacgc caccgaggcg ctcggagtgg
     1321 tcctgattct gaaggcattc tcggccggct gctccgcact gaccggagtc gaggccatag
     1381 ccaacgcggt tccggccttt cgggctccgg cgatcaaacg agcccaaaac tgtgaagtag
     1441 cactgggagt gctgctcggc gtcatgctgg tcgggctggc cgggctgatc cggctacaca
     1501 acatggtgcc ccgcggcgac gtcacgattc tcgcgcaact gacggcggcc gcgttcggca
     1561 ccggctggcc gttttacgtg acgaatctgg cggtggttct ggtgctgggt tgcgcggcca
     1621 ataccagctt cggcggactg ccggtgctgc tgcagttgct ggccaaagac aatcggatgc
     1681 cgcacttgtt catgctgcgc tccgaacgcc cggtgtaccg gtacggcgtc gcggcgctgg
     1741 cgatcctggc gtttctgctc ttgctgatcg tcggcgccga cacccagaag ttgctgcccg
     1801 tttacgcggt cggcgtgttc atcggattca ccatcagtca gacgggcctg gtcaagcatt
     1861 ggcgccaaga acgcgggaaa ggttgggcgc tcaaagccct cctcaacggg agcggcgccg
     1921 cgctcaccgc ggtggcgtcg gtggtgcttt tcgcgaccaa attcaccgaa ggcgcctggc
     1981 tgctgctgat catcgtgccc tcgctgatgc tgctcttcga tcgcaacgaa cgctattacc
     2041 gccgcgtcgg cgaagagctg ggaattggcc gaacaccgat caagccgtcg cccggcgccg
     2101 accctcaggc actggtcatc gttccgatcg tcgcggtcag cgtgctcgcc cagcgtgtgc
     2161 tgcaggcggc gatgcgctta gggggcgaaa tcctgccggt ggcggtcgac gtcgaccccg
     2221 aagacactca acgcctcacc gagcagtgga agcagtggga tcccggggtt gatcttcgag
     2281 tgctgcccag cccgaatcga agcgtggttt tgcccatcac gacgctggtg cgaagccaat
     2341 tgctgcacgg ccggcatgtc accgtcctgc tcggtcaggt cgaacccagc cggcgtcgct
     2401 accggattct gcacaaccag cgtggtctcg tccttgccgc ggcgctgcgc atgcgcacag
     2461 atgcggtcgt cgcgaccctc ctggtgcgcg tcacctagca gcgggtggga tcgccgccac
     2521 cacgcctttt tcggagaccg gaacgactac agcttgcctg cgacgaagtc ggccgcctgg
     2581 ttggccatgc ccgaatctat gtatgcgctg gcgaggtgct gcggccagtt ttgtttccac
     2641 gtgttcgggt cggccgggtt gcagaccgga tccgcgccgt ggcacagctc gatggtcctg
     2701 tcgttgtagg tcgggttgaa gttcgtgatc ggacccaccc actggcttcc gtttccgaac
     2761 aacgcgacgg cggcgatgtg ctgatcggtg cccggcggca gggggctgtc gaaaccgaac
     2821 gcgccgatgg gcgccgcgag caccacgtcg gtgacggcgg cgccgagcga gtagccgccc
     2881 accaccagcc gggtgtccgg gcagttgttg accatgtcct ggatgtggtg gctcatgtcg
     2941 ttggcgccga cgtcgacctc cgtgtcggcg gggtacctga ccgcgtatac gccgatgttc
     3001 ttcgagacct tggactcgag cgcgctgatg aacgcgttgc cgagcacgcc aggccctggc
     3061 gattcgtatc ggccgcgcgc gaagaccacc tgaacatcgg gacaactcgc ggcggccacc
     3121 ggcaccagcg tcccggacgg cagcaccgca ccggtcaatg ccagcgtcgc tgcgaccaca
     3181 gccgcggcca ggccgacgcc ggcccattta cgaagaggca taccaagcac aggacgatcg
     3241 taaagcggtc gcgcccgcgg tgctggccgg ggcaaggcct caggggcgtc accgctggcc
     3301 ggggcggccg tcgaccactc cgtcggcgca gaacgg
//
LOCUS       DASSGQ010000026         8186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_305806,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000026 DASSGQ010000000
VERSION     DASSGQ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8186
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305806"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VFI55_01085"
     CDS             <1..441
                     /locus_tag="VFI55_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072663.1"
                     /translation="AAVTAAGGNVAIVGQPDIGLLAAVEMGADLSRLAVIPDPGTDPV
                     EVAAVLIDGMDLVVLGLGGRLVPQTRARAVVARARIKGCTLLVTDGDWQGAPTRLRAR
                     VCGYEITPGCRGVPAPGFGCISGVRLQVSGVCAGRRVIGRARAG"
     gene            448..2040
                     /locus_tag="VFI55_01090"
     CDS             448..2040
                     /locus_tag="VFI55_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HET7072664.1"
                     /translation="MVSPFGSRVLALWCMDWPAVAAAAAADISATVPVAVTLANRVIA
                     CSSAARAAGVRRGLRRREAAARCPQLHVVTADADRDARFFEGVIAAVDELVPRAEVLR
                     PGLLVLPVRGAARYFGSEQQAAERLIDAVAVSSVAGAECQAGIADQLSTAVFAARAGR
                     VVQPGGDARFLSVLSIRQLATEPSLSGPGREELTDLLWRMGIRTIGQFAALSRTDVAS
                     RFGADGVTAHRFARGEPERGPSGREPPAELEAVLDCDPPIDRVDAAAFAGRSLAGALH
                     QTLMAAGVGCTRLAIHAVTANGEERSRVWRCAEPLTEDATADRVRWQLDGWLSNRRAL
                     DRPTSPVTLLRLQAVEVVSAEALQLPLWGGLGEEDRLRARRALVRVQGLLGPEAVLVP
                     VLSGGRGPAERITLTPLGDEPVPRADPALPWPGQLPEPSPAVLLDDPVELLEAQGNSI
                     RVTSRGMFSADPVRLTLHGRDEQLHWWAGPWPVDERWWDDRPGAGQGGGRTARAQVLL
                     DGERALLLCYRQRRWYLEGSYE"
     gene            complement(2031..3062)
                     /locus_tag="VFI55_01095"
     CDS             complement(2031..3062)
                     /locus_tag="VFI55_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HET7072665.1"
                     /translation="MNPVFVDVDTGVDDALALMYLLASSEADVVGIASTGGNVAVQQV
                     CQNNLGLLELCRSTGIPVSKGSDETLTGPIRAPSRVHGPRGMGYADLPPSDRQLTDYD
                     SATAWVRAAHAFPGELIGVATGPLTNLALALRAEPALPTLLRGLVIMGGSYDHRGNTT
                     AVAEWNISVDPEAAAEVFAAWPVKDVEPQRLPLLCGLNLTRNVAMTPDHLARLAAAAG
                     SPTTVMSASDERGTRSKASNPLIRVIEDAMRFYLEAYNDNGHGYQAHLHDPLAAAAAL
                     DAQLVMPRPATVEVELTGTLTRGMTVTDWSGRRESNALIGIDVDPAVFFDRFIERVGS
                     FARRLDGHS"
     gene            complement(3084..5108)
                     /locus_tag="VFI55_01100"
     CDS             complement(3084..5108)
                     /locus_tag="VFI55_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7072666.1"
                     /translation="MRYVVTGGTGFIGRRVVSRLLETRPDARVWVLVSRPSLGAFGRL
                     AAGWGERAKPLVGELPELDLTDETLAELGHIDHVVHCAAIDHITDITAGEAEQRAISV
                     TDVEATRSVIDLARRLGATLHYVSSIAVAGDFAGEYTEDDFDVGQRLPTAYHQTKFEA
                     ESLVRAATGLRYRVYRPAVVVGDSRTGEMDKIDWPYYFFGVLAKLAALPSLTPILLPD
                     TGRTNVVPVDYVVDALVALMHADGSDGRTFHLTASKTIGLRAIYRVVAKEAGLPPARG
                     SLPRSVTAPVLKARGRARVLRNMAATQLGIPAEIFDLVDLKPTFVSDETRKALRENGI
                     PEAPEFADYAPTLWRYWAEHLDPDRARRDDTSGPLVGRHVIITGASSGIGRASAIAVA
                     ERGATVFALARNGEALDQLVAEIRANGGQAYAFTCDVTDSVSVEHTVKDILGRFDHVD
                     YLVNNAGRSIRRSVVNSTERLHDYERVMAVNYFGAVRMVLALLPHWRERRFGHVVNVS
                     SAGVLARNPKYSSYLPTKAALDAFADVVASETLSDHITFTNIHMPLVRTPMIEPSKRL
                     NPVPAISAERAAAMVVRGLVEKPARIDTPMGTLAEAGNYFTPKLSRRILHQLYLGYPD
                     SAAARGISTAEGGDRPSLHRRPKRPIRAVARMQVPRPVRKAVRLVPGVHW"
     gene            complement(5160..5864)
                     /locus_tag="VFI55_01105"
     CDS             complement(5160..5864)
                     /locus_tag="VFI55_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7072667.1"
                     /translation="MRKRSKILTAVAVLAATVILGACSGSPQVRSITVTFIRHAQSEA
                     NASGIIDTEVPGPGLTPEGKGQAEKVAHDLARNNYDGVYASTMVRTQQTAAPLAAELG
                     KKVEVLQGLQELNAGWYEGKPVSMAPATVLVAPAAWLSGDIQAAIPGSITGKEFNDRF
                     TAAMQKIYDSGQQNPVVFSHLYAIEYWTLLNARNAKDSLLTSHPLPNIGRVVISGNPM
                     TGWTLEDWDGIRNFTN"
     gene            5957..6838
                     /locus_tag="VFI55_01110"
     CDS             5957..6838
                     /locus_tag="VFI55_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HET7072668.1"
                     /translation="MAIDPSAVGAVTEPMLFEWTDRDTMLYALGVGCGTEDLSFTTDN
                     SHDITQQVLPTYAVICCPAFGAAAKIGKFNWAMLLHGSQGIRLHAPLPAAGKLSVVTE
                     VADLQDKGEGKNAIVVLRGRGTDPGSGRLIAETLTTLVLRGEGGFGGQPGQRPVAPEF
                     PDHEPDIQVAIPTREDQALIYRLSGDRNPLHSDPWFARELAGFPKPILHGLCTYGVSG
                     RALVAELGRGLAANITSISARFTSPVFPGETLTTLIWRTEPGKAVFRTGASGVEGASE
                     VDARVVLDDGVVEYVDG"
     gene            complement(6857..8095)
                     /gene="otsB"
                     /locus_tag="VFI55_01115"
     CDS             complement(6857..8095)
                     /gene="otsB"
                     /locus_tag="VFI55_01115"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HET7072669.1"
                     /translation="MEPVIDPRRHDAVLFDLDAVLAADGSALDSAVALVRLLREVGVG
                     AAVFSSNGGRGPSTPAVVGEWLSGDADGPLEAADRLAVRPGRCVVVAANEANITAARA
                     GGFAWVIAVELRANRDALRGLGADAVVADLREVAVRTGDQRMSELPDALQALDSAAGL
                     TAGRPAVFFDFDGTLSDIVEDPDAATPVAGAVEALQQLASQCPVAVLSGRDLADVTKR
                     LGVPGIWYAGSHGFELTAPDGTHHQNDAAAAAIPVLDRAAAELRDRLGSIPGVVVEHK
                     RFGVAVHYRNAARDRVGEVAAAVRAAGQRDGLRVTTGREVIELRPDMDWDKGKTLRWV
                     IDQLHDAGSGSLLPIYLGDDITDEDAFDAVRQDGAGILVRHAEDGDRATAARFALDSP
                     TRVAEFTERLARRVSDSRAN"
BASE COUNT         1215 a         2731 c         2918 g         1322 t
ORIGIN      
        1 gccgcggtga cggctgccgg gggcaacgtc gccatcgtcg gtcagccgga tatcggattg
       61 ctggccgccg tggagatggg ggcggatctg agtcggctcg cggtgatacc ggatcccggg
      121 accgatccgg tggaagtggc cgcggtactc atcgacggca tggatctggt ggtgctcggt
      181 ctgggcgggc gcctggtgcc gcagacacgg gcgcgggcgg tggtggcccg cgcccgcata
      241 aagggctgca ccctgctggt caccgacggt gactggcaag gggcgccgac gcggcttcgg
      301 gcccgggtct gcggttacga gatcactccg ggttgtcggg gggtgcccgc ccccggcttc
      361 ggatgcatca gcggggtgcg gctgcaggtc agcggggtct gtgcggggag gcgggtgatc
      421 ggccgggcgc gagccgggtg aattcggatg gtatccccct ttgggtcccg ggttttagcg
      481 ctctggtgca tggactggcc cgcggtcgcg gcggcggccg cggcggacat atccgcgacg
      541 gtcccggtcg cggtcactct ggccaatcgg gtgatcgcct gctcgtcggc cgcccgtgct
      601 gcgggagtgc ggcgagggct gcggcgccgg gaggcggcgg cccggtgccc gcaactgcac
      661 gtcgtgaccg ccgacgccga ccgcgacgcc cggttcttcg aaggtgtgat cgcggcggtg
      721 gacgagctgg tgccccgcgc cgaggtgttg cgtcccggac tcttggtgtt gccggtgcgc
      781 ggggcggccc gctatttcgg gtccgaacag caggccgccg agcggctgat cgacgcggtg
      841 gctgtgagtt cagtggccgg cgccgagtgt caggccggga tcgccgatca gttgtccacc
      901 gcggtcttcg ccgcgcgtgc gggccgcgtc gtgcagccgg gcggcgatgc gcggtttctg
      961 tcggtgctgt cgatccggca gctcgccacc gagccgagcc tgtccggccc ggggcgagaa
     1021 gaactgacgg acctgttgtg gcggatgggg atccgcacca ttggccagtt cgccgccctg
     1081 tcccgcaccg acgtggcctc caggttcggc gccgacgggg tgaccgcgca ccggttcgcc
     1141 cgcggcgaac ccgaacgggg accgtccggg cgggagccac cggccgaact cgaggccgtg
     1201 ctggactgcg atccgccgat cgaccgggtt gatgccgcgg cattcgccgg gcgctcgctg
     1261 gccggcgcac tgcaccagac gctgatggcc gcgggggtgg gatgcacccg gctggccatt
     1321 cacgccgtca ccgccaacgg cgaagaacgc agcagggtgt ggcggtgtgc cgagccgttg
     1381 accgaggacg ccaccgccga ccgggtgcgc tggcaactgg acgggtggtt gagcaaccga
     1441 agggctctgg accggcccac ctcaccggtg acgctgttgc ggctgcaggc ggtggaggtg
     1501 gtgtccgccg aggcgctgca gttgccgttg tggggcggct tgggcgagga ggacaggctg
     1561 cgggcccgtc gggcactggt gcgggtgcag ggcttgctgg gtccggaagc ggtgctggtg
     1621 ccggtgctgt ccggcggtcg cgggcctgcc gagcgcatca cgttgacccc gctgggcgac
     1681 gaaccggtac cgcgggccga cccggccctg ccgtggcccg gccaactacc ggagccgtcg
     1741 ccggccgtgt tgctggacga tccggtggag ttgcttgaag cccaagggaa ttcgatacgg
     1801 gtgaccagcc gggggatgtt ctcggccgac ccggtgcgac tgaccctgca cggtcgggat
     1861 gagcagctgc actggtgggc cggaccttgg ccggtcgacg agcgctggtg ggacgatcgg
     1921 cccggggccg gtcaaggggg tggccgcacc gcccgtgccc aggtcttgct ggacggcgag
     1981 cgggcgttgc tgctgtgcta ccgccagcgg cgctggtacc tcgaaggaag ctacgagtga
     2041 ccgtcgagcc ggcgtgcgaa cgatccgacc cgttcgatga accggtcgaa gaacaccgct
     2101 ggatcgacgt caatgccgat gagcgcgttg gattctcgac gtcccgacca gtcggtcacc
     2161 gtcatgccgc gggtcagggt gcccgtcagc tcgacctcca ccgtcgccgg gcggggcatg
     2221 acgagctgcg catccagcgc ggccgccgcg gccaagggat catgcagatg cgcctgatag
     2281 ccgtgcccgt tgtcgttgta ggcctccaag tagaaccgca tcgcgtcctc gatcacccgg
     2341 atcaacggat tggatgcctt cgacctggtg ccgcgttcgt cgctcgcgct catcaccgtt
     2401 gtcggcgagc ccgcggcggc cgccagcctc gcgagatggt ccggcgtcat cgcgacgttg
     2461 cgggtcagat tcaagccgca cagcagcgga aggcgttgcg gctcaacatc tttgaccggc
     2521 caggccgcga atacctcggc cgccgcctcg gggtccacgc tgatgttcca ttccgccact
     2581 gcggtggtgt tgccacggtg gtcgtaggag ccgcccatga tcaccagccc gcgcaacagc
     2641 gtcggcagcg cgggttcggc ccgcagcgcc agcgccagat tggtcaacgg gcccgtcgcc
     2701 acgccgatca gctcaccggg aaaggcatgc gccgcccgta cccacgccgt cgcagagtcg
     2761 taatcggtga gctgacgatc actcggcggc aggtcggcgt accccatccc ccgggggccg
     2821 tggactctcg agggggcgcg tatcgggccg gtcagggttt cgtcggagcc cttggacacg
     2881 ggtatgccgg tgctgcggca cagctccagc aggcccaaat tgttctggca aacctgttgt
     2941 accgcaacgt ttccgccggt cgaggcgata ccgaccacat cggcttccga actggccaac
     3001 aggtacatca acgccagcgc atcgtcgacg ccggtgtcga cgtcgacgaa aacgggattc
     3061 accgcggcaa cgataccgtc gctttaccag tgcacgcccg gcaccagtcg gaccgccttc
     3121 cggaccggcc tggggacctg catgcgggcc acggcacgaa tcgggcgttt cgggcgacgg
     3181 tgcaacgagg gacggtcccc gccttcggcg gtcgaaatcc cgcgcgcggc ggccgaatcg
     3241 gggtagccca ggtagagctg gtgcagaatt cggcgcgaca gtttcggtgt gaagtagttg
     3301 ccggcctcgg ccagcgtgcc catcggggtg tcgatccgcg ccggcttctc caccagaccg
     3361 cgaaccacca tcgccgccgc acgttcggcg ctgatcgccg gcaccgggtt gagccgcttg
     3421 gacggctcga tcatgggggt gcggaccagc ggcatatgga tgttggtgaa cgtgatgtgg
     3481 tcggacagcg tctccgaggc gaccacatcg gcgaacgcgt ccagtgctgc cttggtgggc
     3541 aggtacgagc tgtatttggg attacgggcc agcacgccgg cactggacac gttgaccacg
     3601 tgaccgaacc ggcgctcacg ccagtgcggc aacagcgcca gcaccatccg caccgcgccg
     3661 aagtagttga ccgccatcac gcgttcgtag tcgtgcagtc gctcggtgga gttgaccacc
     3721 gagcggcgaa tcgagcgccc ggcgttgttc accaggtagt cgacgtggtc gaagcggccc
     3781 aggatgtctt tgacggtgtg ctccaccgac accgaatcgg tgacatcgca ggtgaaggcg
     3841 tacgcctggc cgccgttggc gcggatctcg gccaccagct ggtccagcgc ctcgccgttg
     3901 cgggccagag cgaacaccgt tgcgccccgc tcggcgacgg cgatcgccga cgcccgcccg
     3961 atgccgctgg atgctccggt gatgatgacg tgccgtccca ccagcggccc cgacgtgtcg
     4021 tcacggcgcg cgcgatcggg gtcgaggtgc tcggcccaat accgccacag cgtgggtgcg
     4081 tagtcggcga actcgggggc ttcgggaatg ccattttccc gcaatgcttt tcgggtttcg
     4141 tcgctgacga aggtgggctt gaggtcaacc agatcgaaaa tctcggcggg aatgcccagc
     4201 tgggtggccg ccatgttgcg aagcaccctg gcccgcccac gcgccttgag caccggcgcg
     4261 gtcaccgagc ggggcaatga tccgcgcgcc ggtggcagcc cggcctcctt ggcgacaacg
     4321 cgatagatgg cgcgcaggcc aatggttttc gaggcggtga ggtggaatgt gcggccgtct
     4381 gaaccatcgg cgtgcatgag cgcgacgagc gcgtcgacga catagtcgac cgggacgacg
     4441 ttggtgcgcc ccgtgtcggg cagcaggatc ggggtcaacg atggcaatgc ggccagcttg
     4501 gccagcacgc cgaagaagta gtagggccag tcgatcttgt ccatctcgcc ggtgcgggaa
     4561 tcgccgacca ccaccgccgg gcggtagacg cggtagcgca gtccggtcgc cgcgcgcacc
     4621 agcgactcgg cttcgaactt cgtctggtga tacgccgtcg gcaaccgctg gccgacgtcg
     4681 aagtcgtctt cggtgtactc gccggcgaag tcgcccgcca ccgcgatcga cgacacgtag
     4741 tgcagcgtgg cgcccagccg gcgcgccaag tcgattacgg agcgggtggc ttcaacatca
     4801 gtgacgctga tggcccgctg ctcggcctcg cctgccgtga tgtccgtgat gtgatcgatc
     4861 gccgcgcagt gcacaacgtg atcgatgtgg cccagctcgg ctagggtctc gtcggtcagg
     4921 tcgagctcag gcagctcgcc tacaagcggc ttcgctcgtt caccccatcc ggcggcgagg
     4981 cgtccgaagg ctccgagcga cgggcgggag accaagaccc acacccgggc atccgggcgg
     5041 gtttccagca gacgagatac gacgcggcgc ccgataaacc cggtaccgcc ggtaacgaca
     5101 taccgcatgg agccatggtg gccgctagcc gccgcctacg tcaaccgtgc ccaccgggct
     5161 cagttggtga agttgcggat cccgtcccag tcctcgagcg tccagccggt catcgggtta
     5221 ccgctgatca ccacgcggcc gatgttcggc agtggatgac tggtcaggag gctgtccttg
     5281 gcgttcctcg cgttcagcag cgtccagtac tcgatggcgt agagatgcga gaacaccacc
     5341 gggttctgct gaccactgtc atagattttc tgcatcgccg ccgtgaaccg gtcgttgaac
     5401 tctttgcccg tgatcgaccc ggggatggcc gcctggatgt cgccgctgag ccaagccgcc
     5461 ggagctacca ggaccgtcgc gggcgccatc gagaccggct tgccctcgta ccagcccgcg
     5521 ttgagctcct gcaggccctg cagcacctcg actttcttgc cgagttcggc ggccagcggc
     5581 gcggcggtct gctgggttcg caccatggtc gaggcgtaga cgccgtcgta gttattgcgc
     5641 gcaagatcgt gcgcgacctt ttcggcctgc ccctttccct ccggcgtcag gcccggaccc
     5701 ggcacctcgg tgtcgatgat cccgctggcg ttggcctctg actgtgcgtg ccggatgaag
     5761 gtcacggtga tggagcgcac ctgcggtgat ccgctgcagg caccgaggat taccgttgcg
     5821 gcgaggaccg ctaccgctgt caagatcttg ctgcgcttgc gcatggcgat agcctgccct
     5881 gccgaaggtg tcgctgtgaa gtgtttgcgt ggttaagtgg cgaaaatggg caaattttcg
     5941 tcgggaggag actcgcatgg ccattgaccc gagtgcggtc ggcgcggtga ccgagccgat
     6001 gctgttcgaa tggaccgacc gggacacgat gctgtatgcc ctcggcgtcg gttgcggaac
     6061 cgaggatctg tcgttcacca ccgacaacag ccacgacatc actcagcagg tgctgcccac
     6121 ctacgcggtg atctgctgcc cggcgttcgg cgccgccgcc aagatcggga agttcaactg
     6181 ggccatgctc ttacacggct cgcaaggtat taggctgcac gcgccgctgc cggctgcggg
     6241 caagctgtcg gtggtcaccg aggtcgccga cctgcaggac aagggtgagg gcaagaacgc
     6301 catcgtcgtg ctgcgcggcc gcggcaccga tccgggttcg gggcgactga tcgccgagac
     6361 gctgaccacg ctggtcctcc gcggcgaggg tgggttcggc gggcagccgg gtcagcggcc
     6421 ggtcgcgccg gaattcccgg accacgagcc cgacatccag gtcgcgatcc ccactcgcga
     6481 ggatcaggcg ctgatctacc gcctgtccgg tgatcgcaac ccgctgcaca gcgacccctg
     6541 gttcgcccgc gagttggccg ggttccccaa gccgatcctg cacgggctgt gtacctatgg
     6601 ggtgtcgggc cgcgcgctgg tcgcggagct cggccggggc ttggccgcca acatcacgtc
     6661 gatctcggcg cggttcactt cgccggtctt tcccggtgag acgctgacca cgctgatctg
     6721 gcgcaccgaa ccgggcaagg cggtgttccg caccggggcg tctggtgtcg aaggcgcctc
     6781 ggaggtggac gcccgcgtgg tgctggacga cggcgtggtg gagtacgtgg acggctaggc
     6841 cccgctgagt gtttcgctaa ttcgcgcggg agtcgctgac ccggcgtgcc agtcgctcgg
     6901 tgaattcggc gacccgtgtc gggctgtcca gcgcgaaccg tgcggcggtg gcccggtcac
     6961 cgtcctcggc gtgccgcacc aggattccgg caccgtcttg acggaccgca tcgaaggcat
     7021 cctcgtcggt gatgtcgtcg ccgagataga tcggcagcaa tgaacccgag ccggcgtcat
     7081 gcagctgatc gatcacccag cgaagggttt ttcccttgtc ccagtccata tccggacgca
     7141 gctcgatgac ctcgcggccg gtggtgaccc gaaggccatc gcgctgaccc gccgcgcgca
     7201 ccgccgccgc cacttccccc acccggtcgc gcgcggcgtt gcgatagtgc acggcaacgc
     7261 caaatcgctt gtgctccacc acaacacccg gaatcgatcc gagccggtcg cgcagctcgg
     7321 cggcggcccg atcgagcacc gggatcgccg ccgccgcggc gtcgttttgg tggtgggttc
     7381 cgtcgggcgc ggtcagctcg aaaccgtggc tgccggcata ccagattccg ggtacgccga
     7441 ggcgcttcgt cacgtcggcg agatcgcggc cggacagcac cgcgaccgga cactgcgagg
     7501 ccagctgctg caacgcctcg accgcgccgg cgacgggcgt ggccgcgtcc ggatcctcaa
     7561 cgatgtcaga gagcgtgccg tcgaagtcga agaacaccgc cgggcgcccc gcggtcaggc
     7621 cagccgccga atccagggcc tgcagcgcat ccggcagctc cgacatccgc tggtcgccgg
     7681 ttcgcacggc cacctcacgc aggtcggcga caaccgcgtc ggcgccgagg cctcgcagtg
     7741 cgtcgcggtt cgccctcagc tcgacggcga tgacccacgc gaagccaccg gcacgcgcgg
     7801 ccgtgatgtt cgcctcgttg gccgcgacga cgacacaccg gccaggccgc accgccagcc
     7861 ggtcagccgc ctcgagcggg ccgtctgcgt cgccggagag ccactcgccg acgacggccg
     7921 gcgtcgatgg gccccggcca ccgttcgacg aaaaaacggc ggcgccgaca ccgacctccc
     7981 ggagcaggcg gaccagtgcc accgccgagt ccagcgcgct gccgtcggcc gccaatacgg
     8041 cgtcgaggtc gaacagcacg gcgtcatgac ggcgcgggtc gatgaccggc tccaagttgg
     8101 gcacgcccca accctctcac ggtgctgggc gccgccggag aaaggtccgg ttgggtcagg
     8161 gtcgtccggg cgcggacggc cgcccg
//
LOCUS       DASSGQ010000027         2444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1278110,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000027 DASSGQ010000000
VERSION     DASSGQ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278110"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..306
                     /locus_tag="VFI55_01120"
     CDS             85..306
                     /locus_tag="VFI55_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072670.1"
                     /translation="MTSRLKAWWRQPDQFDWVTTFLRQREMLGSARMILAVVAGSSVL
                     VPLTVLPSRYQPSALELIAGGIAAIFTLA"
     gene            303..1157
                     /locus_tag="VFI55_01125"
     CDS             303..1157
                     /locus_tag="VFI55_01125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET7072671.1"
                     /translation="MTVLWLTRWPTLRQSQAGVVIGALCIGGWSLVQPTPALAALACT
                     AMAVTGGYIAFFHSPRLLLFNGAVAVSVAIAAVLRLAREFNVATAASAFWVINFINLS
                     IPLAIWGISRAMGTYVQRSEEDPLTGLLNRRAFTDAVTHRLVHSPPAHAYLAVVMIDL
                     DNFKRINDTHGHSVGDRALQAVAASLRAHTPGDAVICRAGGEEFLVALTSVTSDMGPL
                     AAEFCVAFAELSPKITASIGTASTELHLLIGPDVAGLIDELVKTADSAMYVAKRKGGN
                     QAHYGVGP"
     gene            complement(1174..2064)
                     /locus_tag="VFI55_01130"
     CDS             complement(1174..2064)
                     /locus_tag="VFI55_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072672.1"
                     /translation="MRRDPELARRFFDRFEPVHAVTYFAPEARAALEQLGYRGFWMGY
                     FAARSAPLGIVPREVVTAIFYNFAPERVAKALPAAWQIAGPDAALRARRDSAVAALRR
                     YGIGGENVDVAAELAGKAARQAPLDGRPLFAANVALPWPDDPLAALWHAATLLREQRG
                     DAHVAVLAAAGISGRESNVLHAAAGWISRDYIARTRDYDDAAWRHHEQRLAERGLLND
                     DGSLTATGRELKDGIESATDTLALSALDALSDDEVETLFQSLTPITRAVVAGGDIPAV
                     TPMGLHRDELDDGSAHLINT"
     gene            complement(2248..>2444)
                     /locus_tag="VFI55_01135"
     CDS             complement(2248..>2444)
                     /locus_tag="VFI55_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET7072673.1"
                     /translation="RRHAPISLREALGREYSMFMTQHPTTTGLLIVDRDPGRYVEELE
                     NIPPPNPMDHSLRIGGLKGK"
BASE COUNT          395 a          814 c          810 g          425 t
ORIGIN      
        1 ggccgaaatg agtttctgcg ctgaccactg gtcacacgcc ttcgggctga cacagcactc
       61 gatgcggggc aggaggtttg tcgagtgacg tcacggctga aggcttggtg gcggcaaccc
      121 gaccaattcg attgggtgac cacattcctg cggcagcgcg aaatgctggg ctcggcacgg
      181 atgatcctgg ctgtcgtcgc cggatcgtcg gtgttggtgc cgctgaccgt gctgccgagc
      241 cgataccagc ccagcgccct cgaactgatc gccggcggaa ttgccgccat cttcaccttg
      301 gcgtgacggt gctgtggctg acgcgctggc ccaccctgcg gcagtcgcag gccggtgtgg
      361 tgattggcgc gttatgtatc ggcgggtgga gtctcgtcca gcccaccccg gcccttgccg
      421 cgctggcctg cacggcgatg gccgtcaccg gcggctatat cgcgttcttc cacagcccga
      481 gactcttact cttcaatggc gcggtagccg tctcggtcgc catcgcggcg gtgcttcgac
      541 tggcgcgtga attcaacgtg gcgaccgcgg cctccgcgtt ttgggtgatc aacttcatca
      601 atttatctat ccccctcgct atttggggca tatcgcgagc catgggaacg tatgtgcagc
      661 gctccgaaga ggatcctctc accggcctgc tgaaccggcg ggcgttcacc gatgcggtca
      721 cccaccgctt ggtgcattca ccgccagccc acgcctacct cgcggtggtg atgatcgatc
      781 tcgacaactt caaacgcatc aacgacaccc acggtcattc ggtcggcgac cgcgccctgc
      841 aggcggtggc ggcgtcgctg cgcgcgcaca ctcccggcga cgcggtcatc tgccgggccg
      901 gaggcgagga gttcctggtc gcgctgacct ccgtgacctc ggacatggga ccactggccg
      961 cggagttttg cgtcgccttc gccgaacttt caccgaagat aacggccagc atcggcaccg
     1021 ccagcaccga gcttcaccta ctgatcggac ccgatgtcgc cggcctcatc gacgagctcg
     1081 tcaagaccgc cgacagcgcg atgtacgtcg ccaaacgcaa aggcggaaac caggctcatt
     1141 acggcgtcgg gccgtagcag tcgagtgacc tgatcaggta ttgatcaaat gcgcactgcc
     1201 gtcgtctaat tcgtcgcgat gcaatcccat cggcgtgacg gcggggatgt cgccgccggc
     1261 gaccacggcg cgggtgatcg gcgtgagcga ctggaagagc gtctccacct cgtcgtcgct
     1321 gagcgcgtcg agcgccgaca gcgcgagtgt gtcggtcgcc gattcgatgc cgtctttcag
     1381 ttcccgaccc gtggcggtca gggacccgtc gtcgttgagc agaccccgct cggccaaccg
     1441 ttgctcgtgg tgacgccacg ccgcgtcgtc gtaatcgcgg gtacgcgcga tgtagtcacg
     1501 cgaaatccag cctgccgcgg cgtgtagcac gttgctctcc ctacccgaga tgcctgcggc
     1561 ggcgagcacc gccacgtgtg cgtcgccgcg ctgctcgcgt aacagcgtcg cggcatgcca
     1621 tagggcggcc agcgggtcgt cgggccaggg cagcgcgaca ttcgcggcga acagcgggcg
     1681 tccgtcgagc ggcgcctggc gcgccgcctt tcccgctaat tcggctgcga catcgacgtt
     1741 ttcgccgccg atgccatagc gccggagcgc cgcgacggcc gagtcccgtc gggcccgcaa
     1801 cgccgcatcg ggacccgcga tctgccacgc cgccggcagg gccttggcga cccgctcagg
     1861 ggcgaagttg tagaagatcg cggtcaccac ctcccgcggc acgatgccca gcggcgccga
     1921 tcgggcggcg aagtaaccca tccagaaacc gcggtagccc agctgttcca gggcggcccg
     1981 cgcctccggc gcgaaatatg tcaccgcatg caccggctcg aatcgatcga aaaagcgtcg
     2041 ggccagctcc ggatctctgc gcaccgtcgc agttaaccac ccgccgcgac cgcgaccaag
     2101 ggcgaagctg tggatgaggt ggcggcgaca ggacacccgc gcgggcggcg gctcgcgtcg
     2161 cggcacgcgc tgaggtagcg aggacgggta ccccgccgga gtaccgacca gccagcattg
     2221 aggggtctga gcggtcagga cgccagctca tttgcctttc aggccgccga tgcgaagcga
     2281 atggtccatg ggattgggtg gcgggatgtt ctcgagttcc tcgacatagc gcccggggtc
     2341 acggtcgacg atcagcaatc ccgttgtggt ggggtgctgg gtcatgaaca tgctgtactc
     2401 ccggccgagt gcttcgcgaa ggctgatggg ggcatgacga cgga
//
LOCUS       DASSGQ010000028         4723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_670548,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000028 DASSGQ010000000
VERSION     DASSGQ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670548"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1056
                     /locus_tag="VFI55_01140"
     CDS             112..1056
                     /locus_tag="VFI55_01140"
                     /inference="COORDINATES: protein motif:HMM:NF017393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HET7072674.1"
                     /translation="MRIAKSLSVLAVSGLTYAAAGMMPAAVVANPAAAASSVFCSASD
                     LVNGVSQSAAVSGRGSDAREPDLGEVHEDLPATAKGKAGKNFKVTVPVYFHVVTDGAT
                     GAVTNDQIATQINVLNNTYAGGEGGATSGFSFALAGVTRTDNADWFYANPGGTNEHAM
                     KRTLHQGGTGTLNYYSTTAGDYLGWAYLPSIVTKPGQAYLDGVVVDWESMLGTSTRYA
                     GQYDQGETGTHEVGHWLNLEHTFYGGCNAKGDFVEDTPAQKTATRGCPEGKDTCKEPG
                     LDPIHNYMDYSYDSCYTEFTDGQVQRMRDAWLYYRASA"
     gene            1426..1725
                     /locus_tag="VFI55_01145"
     CDS             1426..1725
                     /locus_tag="VFI55_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072675.1"
                     /translation="MAQAVKNLVMDLHDTGCRARFLIRDRDGVPALVNEILADAGIQT
                     VLRGVRVSRMNAIMERWVQSCSHELLDRTLIWNERYATLCSSTSSSTTPIEPTRP"
     gene            2485..4170
                     /gene="acpA"
                     /locus_tag="VFI55_01150"
     CDS             2485..4170
                     /gene="acpA"
                     /locus_tag="VFI55_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HET7072676.1"
                     /translation="MLLLGSLAVIPVPGGPAAAGADRLSRIEHIVVIYQENHSFDNLY
                     GRWEGVNGLNRAPATRTIQVNQAGAPYTCLKQNDVNLTAPPLSARCTDSTTATTFTSH
                     FTNAPFTIDDYIPASATTCPAPGVFAPNGVPNGAGLPGGCTRDLVHRFYQEQYQINGG
                     RQNRYVTGSDAIGLSMGVYNTRALPIYQYLHQPGHPRYAIADSFFQAAFGGSYLNHQW
                     LIAARNPVWPNATNDGTDNDLHSVVDVNGMPNNYELYTSPSTVRDGALTASCDPAPGR
                     GPTPAGILCGDYAVNTIQPPYQPYAPGTPVSRRLPPQTAPTIGERLNAADVDWAWYSG
                     GWSNANGDVGAAGWTNGTGPTCADPNAFATATFPNCPDKLFQFHHQAFNYYANYAPGT
                     AARAAHLRDEQEFRQLARSSTNRSCNLKPVSFVKPIGADNEHPGYTGESGGSAHLVDL
                     LKEIQGSRCAKDTMVVVTYDEFGGQWDHATPPGQAGNRGPHDVFGPGTRLPALVVAPF
                     LRDQFVLDHTQHDTTSILATIEHRFDLPPLSTRDAAVRDLSSVFHADSPWRED"
BASE COUNT          863 a         1705 c         1446 g          709 t
ORIGIN      
        1 gcaagtattc atgatgacgg gcatcaacta cctgcgaacc tccgacgccc gccaaggcaa
       61 tcctccttga ccacaaagca cggagaggta ccgggacgga gaggcaacag catgagaatc
      121 gcaaaatcgc tgtcggtgct cgcggtttcg ggcctgacgt acgcggccgc gggtatgatg
      181 ccggcggccg tggtggcgaa cccggccgcc gcggcgtcgt cggtgttttg cagcgcgtcc
      241 gacctggtca acggggtttc gcagtccgcg gcggtcagcg gtcgtggcag tgacgcccgg
      301 gaacccgacc tcggcgaggt gcacgaggat ctgcccgcca cggcgaaggg caaggccggc
      361 aagaacttca aggtcacggt gccggtgtac ttccacgtcg tcacggacgg cgcgaccggt
      421 gcggtaacca acgatcagat cgccacccag atcaacgtgc tcaacaacac ctacgccggg
      481 ggcgagggcg gcgcgacgtc cgggttcagc ttcgccctgg ccggtgtcac ccgcaccgac
      541 aacgccgact ggttctacgc caaccccggc ggcacgaacg aacacgccat gaagcggacg
      601 ctgcaccagg gcggtacggg caccctcaac tactactcga caaccgccgg ggactacctc
      661 ggctgggcat acctgccgtc catcgtgacc aagcccggtc aggcgtacct cgacggcgtc
      721 gtcgtcgact gggagtcgat gctgggaacg tcgacccggt acgcgggcca gtacgaccag
      781 ggcgagaccg gcacgcacga ggtcggccac tggctgaatc tcgagcacac cttctacggc
      841 ggctgcaacg ccaagggtga cttcgtcgag gacaccccgg cgcagaagac ggcgaccagg
      901 ggctgccctg aaggcaagga cacctgcaag gagcccggcc tcgacccgat ccacaactac
      961 atggactatt cctacgactc ctgctacacc gagttcaccg acggccaggt ccagcgcatg
     1021 cgggacgcct ggctgtacta ccgcgcgtcc gcgtaatcgc cggccgcacc gagcacgccc
     1081 cggcgccggc ccccgtctgg tgggccggcg ctgtcgtcgg atttccgcgt gtgcccgctg
     1141 cgagcgctgc ggtcgggtgc tcctaagctc gcgcagcctc gatctggacc ggcggatgtc
     1201 agcgataccc cccccccggt cttccggcag cggtcgccct gaccatggcc cgcagaatca
     1261 cgacctgggc cgacttcctc cgctcccaag ccgaagcgct gctcgcctgc gattcatcga
     1321 gaccgtcacc ctgaccggcc agcgccagta catccttgcc agcatcgagc acgccaaccg
     1381 gcgtatccgc atcctcggca ccaccgcgca tccgacgcgg gctgggtggc acaggccgtc
     1441 aagaacctcg tgatggatct ccacgacacg ggctgccggg cgcgcttcct gatcagggac
     1501 cgggacgggg ttcccgccct tgtcaacgaa atcctcgccg acgcgggcat tcagaccgtc
     1561 ctccgcggcg ttcgggtctc aagaatgaac gcgatcatgg agagatgggt gcagtcctgc
     1621 agccacgagc tgctcgaccg caccctgatc tggaacgaac gctacgccac gctctgcagc
     1681 agtacgagca gttctacaac gcccatcgag cccaccagac catgatggaa gccgcacccc
     1741 tacgaactgt cccggcaccg atcacagaca taggacgaat cgctcagctc gacatgcgcc
     1801 gacgagaccg actcggcggc gtcgtccacg agtatcaaca tgccgcttga cctgcacgga
     1861 cgagatattc ggcaggcgca atcacgttct tcaaccggtt aacaatccga tagaagccga
     1921 taacggtcaa ttcaagcatc ggctgcgacc gatgcgggga ttgcgtaccg accgcacggc
     1981 gagggtgatc atcgccgggc gtgccttcat gcagaacatc cgacgcggca ctatgcgctg
     2041 ggactcgacg tgccgccggc gttgcaggtc gccgcagcgt tcaccgaact ggcacaggcc
     2101 atctgaccca catgcaaggc gccactcgac gcgtctttga atcagctaat gaaactgtgc
     2161 cgactcggct ccgcacgacg tgagaccgca tcgcggtcgc acggtaggcc cgctgaccac
     2221 acgcttccca cccaacccca gcccgccacg cccacaccaa aagccacgct cggtattgac
     2281 cgaggtgatg taccgggtgt aactggatta aggttctttc gcgcgtgaaa tttcgggtcg
     2341 tcggtttgcc gttcgctgcc gttgtcctat ccgacctgga cactgatgga cggagatcct
     2401 tcagccagca ccggttgaca gcgttaatgc ttggtcgaga tgtgtgggag gtacgtgatg
     2461 tggagacgcc tgtgtgcttc gttcgtgctc cttctcgggt cgctggccgt gataccggtg
     2521 ccaggcgggc cggccgccgc cggggccgat cgcctgtcgc ggattgagca catcgtggtg
     2581 atctaccagg agaaccacag cttcgacaac ctgtacggcc gctgggaggg cgtcaacggg
     2641 ctcaaccggg cacccgcgac gcgcaccata caagtcaacc aggccggcgc cccgtacacc
     2701 tgtctcaagc agaatgacgt gaacctcacc gcgcccccgc tgtcggcgcg gtgcaccgac
     2761 tcgacgaccg ccacgacgtt caccagccac ttcacgaacg caccgttcac gatcgacgac
     2821 tacattccgg cgtccgcgac cacctgcccg gctcccggcg tattcgcgcc gaacggtgtg
     2881 cccaacggcg ccgggctgcc gggtggctgc acacgcgatc tcgtgcaccg gttctaccag
     2941 gagcagtacc agatcaacgg cgggcgacag aaccggtacg tgaccggaag cgacgcgatc
     3001 ggattgtcca tgggcgtcta caacactcgc gccctgccga tctaccagta cctgcaccag
     3061 cccggtcacc cgcggtacgc gatcgcggac agcttcttcc aggccgcctt cggcggctca
     3121 tatctcaacc accagtggct gatcgccgcc cgcaacccgg tctggccgaa cgcgaccaac
     3181 gacggtaccg acaacgacct gcactccgtc gtcgacgtaa acgggatgcc caacaactac
     3241 gagctgtaca cgtcgccgtc gacggtccgc gacggggccc taacggcgtc ctgcgacccg
     3301 gcgcccggcc gcggtccgac cccggccggt atcctctgcg gcgactacgc cgtcaacacg
     3361 atccagcccc cctaccagcc atacgccccg ggcacacccg tctcgcgtcg gctcccaccg
     3421 caaaccgcgc ccaccatcgg cgagcgtctc aacgccgctg atgtcgactg ggcctggtac
     3481 tcagggggat ggtcgaacgc caacggcgac gtcggcgcgg ccggttggac caacggcact
     3541 ggcccgacgt gcgcggaccc gaacgcgttc gccaccgcca cgttcccgaa ctgtccggac
     3601 aagctgttcc agttccacca ccaggcgttc aactactacg ccaactatgc gcccggaacc
     3661 gcggccaggg cggcgcacct gcgcgacgag caggagttcc ggcaactcgc ccggtcgtcc
     3721 accaaccggt cctgcaacct caagccggtg agcttcgtca agccgatcgg cgccgacaac
     3781 gagcaccccg gctacacggg tgagagcggc ggcagcgccc acctggtcga cctgctcaag
     3841 gagatccaag gtagccgttg cgccaaggac accatggtcg tggtcaccta cgacgagttc
     3901 ggcggtcaat gggatcacgc caccccgccc ggccaggccg gcaaccgcgg tccgcacgac
     3961 gtcttcggac ccggaacacg gttacccgcg ctggtggtcg caccgttcct ccgcgaccag
     4021 ttcgtcctcg accacaccca acacgacacc acctcgatcc tcgccacgat cgaacaccgg
     4081 ttcgacctcc cgccgctgag cactcgcgac gccgcagttc gggatctgtc cagcgtgttc
     4141 cacgccgaca gcccctggcg agaggactga tcgacaaggg tgcacgcgtc gccggtgaca
     4201 cgaaaccggc ggcaccgccg ccctggcccg ggcgccatca gccgggcccg ggcggcggcc
     4261 tttccaccga ttggcccggc atggtggccg tcgatatcag cccgtggctg cggccgaagg
     4321 cggtcacgtc gccagctacg acgtcagccg gctggcgcga actcgaagcc gttggacgtc
     4381 ggctttccgc cggcccgtgc cggcgcgagg tcctgttctc cggcgccggc gcgctgcagg
     4441 agatccttgt cacgagcgtc gcgagcagtc ccgcttccgt aacaccgatc gaaacgatcg
     4501 ccgccgccct cgaagccgct gggggcctgc tccagcggcg tcgcgaatca tcgcggcggc
     4561 gctagagcct gatcgacgca aaccccgagc cgcaggaacg cgaactgctc aagctcgcat
     4621 ccctcgcctc ggcgctggcc gatgcgttgc gccggcgggg cgtcggggag ccggccgcga
     4681 gcctgaccgc gcaggcgggg attgcggtct tcaaggtcgc ctt
//
LOCUS       DASSGQ010000029         3524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_973908,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000029 DASSGQ010000000
VERSION     DASSGQ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973908"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFI55_01155"
     CDS             complement(<1..227)
                     /locus_tag="VFI55_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072677.1"
                     /translation="MTSLNFDIYLRSTPERVWAVLTDPRKVPAGRFGMSSHTDWHARH
                     QVPGQTIEDETDSESAAERRLTCEWLQTEHLT"
     gene            complement(224..493)
                     /locus_tag="VFI55_01160"
     CDS             complement(224..493)
                     /locus_tag="VFI55_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072678.1"
                     /translation="MTDPGVPHNDMLSFLWRSRMDIPQIGDEALDALLAATVGGGGKV
                     AAYCASAPRPGVGAHVKWASHATGRATSRPGHTPAGKHKGKAASR"
     gene            complement(657..1877)
                     /locus_tag="VFI55_01165"
     CDS             complement(657..1877)
                     /locus_tag="VFI55_01165"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HET7072679.1"
                     /translation="MAFDTKGRVYQTTLPFNAFWGGSTLHPDGAIDVSYSDDMGQHWV
                     KGNGGQDLEQSPNASAKQAGHVEDKQWIAVNDIAGSPNQDHVYAMWSVFNSSTTKIRI
                     AVSRDRGKTFSKAVTITAPSQTGPSNTYIYPSVDAAGTLYIAFASFPQNGKTSTATLY
                     VSHSTDDGQTFAPFARAATAGVLPAANLPNTTFRDGITENFTASPTYPGHLYLTYENW
                     DGTQMDVKFTQSTDGGSTWSTPTTVNDNVDAAGVPTDQFQPSVAAGPNGAVAVAFYDR
                     RLPCPNDPSILPADIGRTNFCIDVSLQAYKDSGNGAKLVGGNVRITNSGWDPQQPGQT
                     VGGLSQLPCADANCAVGFIGDYFGLAVSRGNIYALFVSTHYPSDVTADGGGPVYYQQQ
                     VLATVPRSDFGTGF"
     gene            complement(2216..3043)
                     /locus_tag="VFI55_01170"
     CDS             complement(2216..3043)
                     /locus_tag="VFI55_01170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET7072680.1"
                     /translation="MTQGTPPARTDSSKLPPFDPSVPNPARMWNYWLGGKDNFAADRE
                     AAERVLEAMPSLPLIARAARLFLIDAVHQLAAEHGVRQFLDIGTGLPTADNTHDVAQR
                     AAPESKIVYVDYDPVVLTHAQALLTSSPEGVTDYIQADLRDTDTIFKAASRTFDLTHP
                     VAVLLIAVLHFIPDADDPYAVVARLMDAVPPGSYLVIAHAAADIAPEASAEMARRYNQ
                     MASATVTPRTRDQVARFFTGLDLLPPGLVPINQWALTGHIDATTGALVGYSAIARKP"
     gene            complement(3172..>3524)
                     /locus_tag="VFI55_01175"
     CDS             complement(3172..>3524)
                     /locus_tag="VFI55_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007297569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7072681.1"
                     /translation="DIGTVGIPVSDQDKAVEFFTGTLGFEKRLDARMGESFRWVTVAA
                     PGASTSVALIARPDTGADTGIRFLVPDAETEHAAMRQRGIEVGDLLRWPGVPPMFEFK
                     DPDGNRFEIVEASA"
BASE COUNT          514 a         1059 c         1306 g          645 t
ORIGIN      
        1 gtgaggtgtt ctgtctgcag ccactcgcac gtcaaccgtc gctccgcggc tgattcggag
       61 tccgtttcat cctcgattgt ctggccggga acctggtggc gtgcatgcca gtcagtgtgg
      121 gatgacatgc cgaagcgccc ggccggcact ttgcgcggat cggtcagaac tgcccagacc
      181 cgctcgggcg tggagcgcaa gtagatgtca aaatttaggc tcgtcatcgt gatgccgcct
      241 tgcccttgtg cttgccggcc ggtgtatggc caggccggga tgtggcccgg ccggtggcgt
      301 ggctggccca cttgacgtgt gcgccgaccc cggggcgggg tgcgctcgcg cagtaggccg
      361 ccaccttgcc gccgccgccg actgtggcgg ccagaagagc atccagagcc tcgtccccta
      421 tctgggggat gtccatcctg gatcgccaga gaaagctcag catgtcattg tgcggaacgc
      481 cggggtccgt catagcgtta cacctgcgtt ggccaggatt cctgcaagcc gccgaaggcc
      541 gcaatgggca gccaccctag gccacgaacg ggttgccgtt ctgcggcagt ggtcaggtgg
      601 gcgcctgctc gccgaggcac gagcggtgtt ccgtgccctg tcggcggggt ccgggttcag
      661 aatccggtgc cgaagtccga tcggggcacg gtggcgagta cctgctgttg gtagtacacc
      721 ggccctccgc cgtcggcggt cacgtcagat ggatagtgag tcgatacgaa cagcgcgtag
      781 atgttgcctc tggagaccgc caggccgaag tagtcgccga tgaatcccac ggcacagttg
      841 gcgtcggcgc acggaagctg gctcaagccg ccgaccgtct gaccaggctg ctgcggatcc
      901 cagccagaat tcgtgatccg cacgtttccg ccaacgagct tcgcgccgtt gcccgagtcc
      961 ttgtatgcct gcagcgagac gtcgatgcag aagttcgtcc tgccgatgtc ggcgggcagg
     1021 atgctcgggt cgttcgggca cgggagacgg cggtcgtaaa acgcgacggc gaccgcgccg
     1081 ttggggccgg cggccacgga tggctggaac tgatcggtcg ggacaccggc ggcgtcgacg
     1141 ttatcgttca ccgtggtcgg cgtcgaccag gtgctgccgc cgtcggtcga ctgggtgaac
     1201 ttgacgtcca tctgggtgcc gtcccagttc tcgtaggtga ggtagaggtg gccggggtag
     1261 gtcgggctcg cggtgaagtt ctcggtgatc ccgtcccgga aggtcgtgtt cggcaggttc
     1321 gcagctggca gcacgccggc cgtggcggct cgggcgaacg gggcgaatgt ctggccgtcg
     1381 tcggtggagt ggctgacgta cagcgtcgcg gtcgacgtct tgccgttctg cgggaacgag
     1441 gcgaaggcga tgtacagcgt gcctgcggcg tcgaccgagg ggtagatgta cgtgtttgag
     1501 gggccggtct ggctgggtgc ggtgatcgtt accgccttgg agaaggtctt tccccggtcg
     1561 cgggagacgg cgatcctgat cttggtggtt gagctattga ataccgacca cattgcgtag
     1621 acgtggtcct ggttggggct gccggcgatg tcgttgaccg cgatccactg cttgtcctcg
     1681 acatgacctg cctgcttagc cgaggcgttc ggggactgct ccaggtcctg cccgccgttg
     1741 cccttgaccc agtgctggcc catgtcgtcg gagtaggaga cgtcgatcgc tccgtcggga
     1801 tgcagcgtcg agccgcccca gaaggcgttg aacggcagcg tcgtctggta gacacgtccc
     1861 ttggtgtcaa acgccacgtt cgggtcggta aggttcgtcc aactcggcgg catcgcctgc
     1921 gttcctgtcg acacgcagtc gtacccctgc acctggttgt caccggtcgg cgtgccgccc
     1981 aggatgcggt agctgccaag ggagtggtcg tagaaacgat gatcgcgaac cggaagaagt
     2041 acatgatcgg tccttcccgc tcaaacccat acgaacgcta cgccgggggc cgaccgcacg
     2101 gaaggccaca tgttgtcaca aatggcgacg tccttgcctc gggggcggga acgtaggccc
     2161 gttcggcatc aaattgcggc gggacggcgg tcacctttgg ccgctcaggg gccggtcagg
     2221 gtttgcgggc gatggcgctg tagccgacca gggcgccggt ggtggcgtcg atgtgaccgg
     2281 tgagggccca ctggttgatg gggaccaggc cgggtgggag caggtccaag ccggtgaaga
     2341 agcgggcgac ctggtcgcgg gtgcgcggag tgaccgttgc ggacgccatc tggttgtagc
     2401 ggcgggccat ctcggcggag gcttcggggg cgatgtcggc cgcggcgtgg gcgatgacca
     2461 ggtagctgcc gggggggacg gcgtccatca gccgcgccac gaccgcgtag gggtcgtcgg
     2521 cgtcggggat gaagtgcagg acggcgatca gcaggacagc gaccgggtgg gtgaggtcga
     2581 aggtgcggga cgcggccttg aagatggtgt cggtgtcgcg caggtcggcc tggatgtagt
     2641 cggtcacgcc ttccgggctg ctggtcagca gggcctgggc gtgggtgagc accaccgggt
     2701 cgtagtccac gtagacgatc ttggactcgg gggcggcgcg ctgggcgacg tcgtgggtgt
     2761 tgtccgcggt gggcaggccg gtgccgatgt cgagaaactg gcggacgccg tgttcggccg
     2821 ccagctggtg caccgcgtcg atcaggaaca gccgcgcggc gcgggcgatc agcggcaggg
     2881 agggcatcgc ctcgaggacc cgttcggccg cttcccggtc ggcggcgaag ttgtccttgc
     2941 cgcccagcca gtagttccac atccgcgccg ggttcggcac gctggggtcg aagggcggca
     3001 gcttgctgct atcggtccgg gccggtggcg tgccctgcgt cacttgtctc ccttcctgcc
     3061 cgcccgttcg gtcatcggtc aaagcttacc ggcgggtatg tgtcaccggt gtgcatgggg
     3121 gcccggccgc cagttggtga ggacctgcgc aaggtgacgg cgaggcggaa ctcaggcgct
     3181 ggcttcgacg atctcgaacc tgttcccgtc cgggtccttg aactcgaaca tgggcgggac
     3241 acccggccac cggagcaggt ccccgacttc gatgccgcgc tggcgcatgg cggcgtgctc
     3301 ggtttcggcg tcgggaacca ggaaccggat gccggtatcg gccccggtgt caggcctggc
     3361 gatcagcgcg accgacgtgg acgcgccggg ggccgccacc gtgacccagc ggaagctctc
     3421 acccatccgt gcgtcgagcc gcttctcgaa gccgagcgtg ccggtgaaga actccaccgc
     3481 cttgtcctgg tcgctgaccg ggattcccac ggtgccgatg tcgg
//
LOCUS       DASSGQ010000030        27870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1004567,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000030 DASSGQ010000000
VERSION     DASSGQ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 27870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27870
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004567"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VFI55_01180"
     CDS             complement(<1..902)
                     /locus_tag="VFI55_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Mce family protein"
                     /protein_id="HET7072682.1"
                     /translation="MRTLEPPNRLRLGLMGILVLLLAIGVGQSITSVPMLFAKPSYYG
                     QFTDSGGLINSDKVRIAGMDVGRIEGITIQDDHVLVKFSLGTQTIGTESRLAIRTDTI
                     LGKKVLEVEPRGAETLRPGATLPLGQSTTPYQIYDAFFDVTKAATGWDIDTVKQSLHV
                     LSQTVDETYPHLSATLDGVAKFSDTIGKRDEEIKHLLAQANQVASVLGDRSEQVDRAL
                     VNAQTLVAAFDERGQAIDALLGNVAAFSQQVKGLIKENKNLTHVLEQLQTISDILVQR
                     KEDLAKGLTEVGAFLPSLNESIGSG"
     gene            complement(899..1939)
                     /locus_tag="VFI55_01185"
     CDS             complement(899..1939)
                     /locus_tag="VFI55_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Mce family protein"
                     /protein_id="HET7072683.1"
                     /translation="MKNTGTLIRLGIVSTVLLLFTVMIIVVFGQLRFDRTTGYSAEFS
                     NISGLRQGQFVRASGVEIGKVASMHLIDGGKRVRVDFNVDRSVPLYQSTTAQIRYLDL
                     IGNRYLELKRGEGEGADRILPPGGFIPLARTSPALDLDALIGGFKPLFRALDPQKVNA
                     IASALITVFQGQGGTINDILDNTAQLTTQIGERDQAIGEVVKNLNVVLDTTVRHRKEF
                     DQTVNNLEVLITGLKDHADPLAAGTAHISNVSGTVADLLAEDRALLHKTINYLDAVQQ
                     PLIDQHDQLNDYLHKIPTALNLIGRGIGSYGDFVNFYACDITLKINGLQGGGPVRTVR
                     LFNQPSGRCSPQ"
     gene            complement(1936..3240)
                     /locus_tag="VFI55_01190"
     CDS             complement(1936..3240)
                     /locus_tag="VFI55_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HET7072684.1"
                     /translation="MTAPAGKVNAARTPPYKAAGLVLVVVSVLVLWLVYMQFRGNFTP
                     KTHLTMLSTRAGLVMDPGAKVTYNGVEIGRVATISEVSQDGKPAAKFRLDVYPRYLQL
                     IPANVDANIRTSTLFGGKYVSLTAPKNPSPQRITPQNVIDARSVTTELNTLFQTVTSI
                     AEKVDVVKLNLTLSAAAQALTGLGDKFGESLVKSNVILDDVNPQMPQIRHNFSQLANL
                     ADVYANASPDLFDFLNNAVVTSRTINQQQKDLDQALLSAAGFGNTGADIFERGGPYLA
                     RGAADLVPTAQLLDTYSPAVYCTIHNYHDIEPKAAAYLGGNGYSLNDHAELLSGLGLF
                     LNPVAAVAAIGSMFGGLAGVVGGAPNPYIWPENLPRVNARGGPGGAPGCWQHITHDLW
                     PAPELVMDTGNSIAPYNHLDTGSPYAIEYVWGRQVGDNTINP"
     gene            complement(3246..4115)
                     /locus_tag="VFI55_01195"
     CDS             complement(3246..4115)
                     /locus_tag="VFI55_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7072685.1"
                     /translation="MSTAQVFRARFPQASAAVNRYAGATTRTLDTLGQMGWFNVVAIT
                     DTPFAMRRYRKETLRLIAQIGMGTGAMAVVGGTVAIVGFVVLSASSLVAIQGFASLGY
                     LGIEVFTGFFAALINVRFVAPVVCGIALAATVGAGATAELGAMRISEEIDALEVMGIK
                     SIAFLASTRIVAGFVVIIPLYSLAMLMSFISPQIVTTVLYGQTSGTYDHYFRTFLRPG
                     DAFWSFVEVLIISVVVMTTHCYYGYNAGGGPVGVGEAVGRSMRFSLVSVNVVVLLIAL
                     AIYGVNPNFALAV"
     gene            complement(4117..4917)
                     /locus_tag="VFI55_01200"
     CDS             complement(4117..4917)
                     /locus_tag="VFI55_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7072686.1"
                     /translation="MTATQPRLGSYLRDQMQTPLTMIGGFFRMCVLTGKALFRFPFHW
                     GELLEQCWFITRVAFLPTIMVSIPVTVMFIFTLNILLGEVGASDISGAGAAIGTVTQI
                     GPIVTVLVVAGAGSTAICADLGARTIREEIDALEVLGIDPIHRLVVPRVIAATLVATL
                     LNALVITVGLVGGFIFGVFLQNVSPGAYLSTLTLITGLPEVVIAEVKAATFGLIAGLV
                     GSYRGLTVRGGAKGLGTAVNETVVLCVVALYAVNVVLTTIGIRFGTGH"
     gene            complement(5121..6780)
                     /gene="fadD5"
                     /locus_tag="VFI55_01205"
                     /pseudo
     CDS             complement(5121..6780)
                     /gene="fadD5"
                     /locus_tag="VFI55_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid--CoA ligase FadD5"
     gene            6947..7639
                     /locus_tag="VFI55_01210"
     CDS             6947..7639
                     /locus_tag="VFI55_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7072687.1"
                     /translation="MNTPISMRPKTQRPLRRAQLSDEVAGHLRAAIMSGTLRPGTFIR
                     LDETAAELGVSVTPVREALLKLRGEGMVQLEPHRGHVVLPLTRQDVADIFWLQATIVR
                     ELAAAATDHITDAEIDELERINDALATAVGSGDAEATAGNEFAFHRAFNQAGGRIKLA
                     WFLLNAARYMPVLVYAADPRWGAAAVDSHRRLIAALRRRDAAAVIEHTVWQFTDQANR
                     STEMLDRAGVFG"
     gene            complement(7648..8088)
                     /locus_tag="VFI55_01215"
     CDS             complement(7648..8088)
                     /locus_tag="VFI55_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7072688.1"
                     /translation="MPVLSKTVEVSADAATIMAIVGDFEAYPQWNESIKGLWVLARYD
                     DGRPSQLRLDTEVQGMHSTFIQAVYYPGANQIQTVLQQGDLFSKQEQLFSVVGTGASS
                     LLTVDVDVETTMPVPAPVVKQLLHNVLDHLAETLKQRAEQLSGN"
     gene            complement(8181..8702)
                     /locus_tag="VFI55_01220"
     CDS             complement(8181..8702)
                     /locus_tag="VFI55_01220"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET7072689.1"
                     /translation="MASGPSVPKVPAVPPAPDELTSDDFPLLWPVGTRWADNDMFGHL
                     NNAVYYEIFDTAINAWINTTVSVDPMTASALVVVAESGCRYMSELHFPETLAVGISVT
                     RLGRSSVTYRLGVFRADHRQPGNGSGGPTAGGEPIAAVGHWVDVYVDRASRKPVPIPD
                     AIRSLLSTVCVDH"
     gene            complement(8790..9383)
                     /locus_tag="VFI55_01225"
     CDS             complement(8790..9383)
                     /locus_tag="VFI55_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072690.1"
                     /translation="MVTSTFGISQNSRIAVGPATRAEGECLKYRLDVIAASAADVVAS
                     AGGWLYDRAMAGWEVTVLLQHSCDTRSLRILGVQALDPESEPAARFALTGPTSQSLAV
                     SAEAFTTDARVRAKVLESLDDRLTEVALWGRAPQGWPLGVNRAMTRAQHVLSAAARKF
                     KGYALAAAGIGCQSVDPTETLLCDGRSPVVSGLIRLD"
     gene            complement(9566..10747)
                     /locus_tag="VFI55_01230"
     CDS             complement(9566..10747)
                     /locus_tag="VFI55_01230"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET7072691.1"
                     /translation="MAGPVQGIKVVELGVWVAAPAAGGILADWGADVIKIEPPTGDPG
                     RMFGRMLGCDLGVNPPFEMDNRSKRSIVLDLTGAAGRDTAFELLSDADVFLTNVRPGA
                     LVRLGMDFASVSARNPRLVYGLITGYGETGPDADRAAYDVAAFWSRAGVAHLLTRPGD
                     TPPFQRGGMGDHSAGMTLAAAVCAALLARERTGTGQLVTTSLYRQGVYTVSFDLNTYL
                     MTGQPIAIGQRESMGNPCMNNYAAGDGRRFWIVGLQGDRHWPPLCRVVGHPEWGDDPR
                     FRDARDRAANAVQLIAELDEIFATRTLDEWAQIFAGEPDFFWSPVNTLEDVVADEQFY
                     AAGGLVDVPVDAPDGEVSVGMVATPADFHGTPWAPRSAAPQLGQHTDEVLAELKARSG
                     S"
     gene            complement(10809..11426)
                     /locus_tag="VFI55_01235"
     CDS             complement(10809..11426)
                     /locus_tag="VFI55_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072692.1"
                     /translation="MTYPAWAEQTAHRTSEIRPTAGWPARINAMLRPLVVAVGIITAG
                     ATSLPMAHADPPPGVPPNNIGFGNNGPTSNALAGMGQSICPMLVQPGATVASIASQLS
                     GSTGLPPGLVGLAASMAIQMGCPGFMNSLANGQIPGPLQGPLQGLAGNPGSPSPFQLP
                     GANPAQGVPLSPPGANPAPGVPPSLRGTGPAVPAAATSELRMPFM"
     gene            11697..12392
                     /locus_tag="VFI55_01240"
                     /pseudo
     CDS             11697..12392
                     /locus_tag="VFI55_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874179.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
     gene            complement(12372..13562)
                     /locus_tag="VFI55_01245"
     CDS             complement(12372..13562)
                     /locus_tag="VFI55_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7072693.1"
                     /translation="MDFSRVELCAEDQDFLDQTRAFISKHVTDEVLRHDREFGENFDE
                     DVHLALGEAGYLASDWKQESEGGFSPVRRRIFALEIARAHTPWYHWGTTSAVARLVQQ
                     FGAPELAGKVIPGVLSGEIRLCLGYTEPEGGSDVATCKTRAVRDGSADGAWVINGSKM
                     FTSNAQNSRYVFLLTNTDPEGPKHKNLTMFLVPLDSPGIEIQGVRTLDGDRTNIVYYS
                     DVRVDDLYRIGEVNGGWTVMRSALASEHGIVDPEDHGLQHIAAMAGHGDVMAEVVDTV
                     AAIAGRAGPDGRRQLDDDSVKYRLGRSFARMEAALSTPGMFGRVAIAQTMRDISGDMM
                     DILGTASALPTDAHGSAGDGAAEHLYRLALPLGIYGGTLEVFRNMIAQHELKLGRPTT
                     AGER"
     gene            complement(13563..14477)
                     /locus_tag="VFI55_01250"
     CDS             complement(13563..14477)
                     /locus_tag="VFI55_01250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET7072694.1"
                     /translation="MYFTITHPIHTHPYNPELLSGEGIATVAAAAEAAGIHGFGFTDH
                     PAPSQRWLEAGGHDALDPFVALGFAAARTTTLRLIPNIVVLPYRNPFVVAKSGASLDL
                     LSGGRFTLAVGVGYLKREFAALGVSYDERAELFDEALQVIKAIWTSDDISFEGKHFSA
                     RGVTAHPRPISSPPIWIGGNTAASRQRVAQHGDGWCPFPAPARLAQTAGTAVIDSVQR
                     LGETIDDLRRRCDAAGRDWSAIDITFANFAGGSPANDEFNADAYLDGLEKLAALGVTW
                     VSVGLPGDSMVHALETLQRFRTLVIDAI"
     gene            complement(14494..15393)
                     /locus_tag="VFI55_01255"
     CDS             complement(14494..15393)
                     /locus_tag="VFI55_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HET7072695.1"
                     /translation="MTNISRESEQAQWSVESLLGLFDVRADGPDRFVGNTGGDVGAGQ
                     DGRQVVEGTQVLAQAIVAAAKRFPDKSVRSAHAVFSRAVAVGPPIELVLDVVAEGRST
                     ATAVVAVHQHGRRCLSITVLADVPTGDVIRHQLPLPDVAAPADANHCEMPMTGRQLRL
                     VDVVDVNSPDEVGPPELYAWLHYDSIPTRDDLAKALVAYFTGHLGISTTMRAHEGIGT
                     SQAHLTVSTAPMSISVAFHEPVRWDGWLLYTHESTQVGAGMSYVRGTVHAQDGELLCS
                     FAQEALIRPLRTADNAIDSRARF"
     gene            15540..16358
                     /locus_tag="VFI55_01260"
     CDS             15540..16358
                     /locus_tag="VFI55_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET7072696.1"
                     /translation="MTPEPLANGFCFGEGPRWFEGLLWFSDRLGAAVHTADMRGTLTT
                     LPLPGHSPSGLGFRPDGSLLIASTEDRQVLRYDGECVVGIADLTGLAPAHLGDMVVDD
                     EGRAYIGSQAFSAGVIIRLDPDDTATVVAEDLDFPNGMVITPDRTTLIVAESTGRRLT
                     AFSIGEGGALGERRVFADGLDGPPDGIALDADGGVWTSMTLAHQFARIVEGGGVTDRI
                     DMGERVAIACALGGPTRRTLFLLSSTDAYPQHLVGTRLSRLDAVQVGSPGAGLP"
     gene            16380..17201
                     /locus_tag="VFI55_01265"
     CDS             16380..17201
                     /locus_tag="VFI55_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET7072697.1"
                     /translation="MTDSYYELIDGTDAQGEKFRATDLARGTWSAAIQHGGPVSALLV
                     RALERCERRDDTRLSRVVIDLLGGVPADGDLWVSSQLLRGGKQIELVGAEILAPGPDG
                     DPRPVARASGWRLQQQDTRSLTHAAAPLPRPRTEASSRNMKALMWDRNYVHSLDWLWL
                     TEPLTAGPGESWITPTVDLVGGEAMTPLERLFAVADCANGIGSKLNITKWTFLNIDLA
                     VHVFRIPDGEWIGIRAETSYGPDGVGTTIGTLFDEQGAVGAIQQSVLVRRRPGRS"
     gene            17218..17766
                     /locus_tag="VFI55_01270"
     CDS             17218..17766
                     /locus_tag="VFI55_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7072698.1"
                     /translation="MSQAPPADTTVTDTSTRQRILAATAEVLGRNGKTKLSLSDVANQ
                     AGVSRPTLYRWFASKEELLSAFSRYERQVFESGLAKATAGLKGTDRLDAALRFIVEYQ
                     HSYSGVRMVDVEPEHVIAQVSRAIPQMREGLERLLPGPNAAVKAATVIRIAISHYIVR
                     SDDAAQFLAQLRHAVGIKAPAE"
     gene            complement(17810..19162)
                     /locus_tag="VFI55_01275"
     CDS             complement(17810..19162)
                     /locus_tag="VFI55_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7072699.1"
                     /translation="MADQLANHLANIVGSTHVITDPDVLAGRSVDHTGRYRGRASALV
                     RPGSPEQVAEVLRVCRDAGAHVTVQGGRTSLVAGTVPEYEDVLLSTERLSAVGDVDVV
                     ERRVAAGAGATLAAVQLAAAAAGLVFGVDLAARDTATVGGMASTHAGGLRTVRYGNMG
                     EQVVGLQVALPDGSLMRRHSLVRRDNTGYDLPALFVGAEGTLGVITSLDLRLHPTPSH
                     RITAVCGFADLDALVDAGRTFRDVDGIAALELIDGRAAALTAEHLGVGAPVAGDWLLL
                     VELAADQDQTDRLGELLDGLQLCGEPAVGVDVAAQQRLWRLRESLAEVLGVYGPPLKF
                     DVSLPLAAIAEFARDATALIEARAHDALRLLFGHIGEGNLHLNVLRVPLERERELYAP
                     MMDLIAGSGGNVSSEHGVGSRKRAYLGMSREAADIAAMRTVKAALDPTGYLNAAVLFD
                     "
     gene            complement(19211..19951)
                     /locus_tag="VFI55_01280"
     CDS             complement(19211..19951)
                     /locus_tag="VFI55_01280"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET7072700.1"
                     /translation="MGRVAGKVALISGGARGMGAEDARLLVAEGAKVVIGDILDNEGK
                     ALADEIGDAARYVHLDVTRPDQWDAAVATATGEFGKLNVLVNNAGIVALGQLKNFDLA
                     KWQKVIDVNLTGTFLGMRVAVEPMTAAGGGSIINVSSIEGLRGAPAVHPYVASKWAVR
                     GLAKSAALELAPLNIRVNSIHPGFIRTPMTAKLPDDMVTVPLGRPGEAREVAMFVVFL
                     ASDESSYATGSEFVVDGGLVSDVPHKQL"
     gene            complement(19969..21057)
                     /locus_tag="VFI55_01285"
     CDS             complement(19969..21057)
                     /locus_tag="VFI55_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HET7072701.1"
                     /translation="MAIRVAHVGTGNVGRLALAGLVTSPRFKLTGLCVSTPEKVGKDA
                     GALCGAAAVTGVAAVDDLDAVLATGPECVVYCAMGDTRLPDAMKDVMRILAAGVNVVG
                     SSPGMLQFPWGVLPDKYIARVEDAAQQGNSSIFINGVDPGFINDLIPFALSGTCQRIE
                     QVRCMEIADYATYDGAEVMHYMGFGRPLDDVPMLLLPGVLSIAWGGAIRQLAAGLGIE
                     LDGITESYQREPAPEAFDIAVGRVAEGTMAALRFEIRGMSNGRPAIVIEHITRLRPDL
                     RPDWPQPASGGGSYRVEIVGEPSYAVDIVPSSRKGDHNHAAIVGAAGRIVNAIPAVIA
                     APPGIRTTLDLPLVSGKGLFAPNSLVAT"
     gene            21258..22766
                     /locus_tag="VFI55_01290"
     CDS             21258..22766
                     /locus_tag="VFI55_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PPE domain-containing protein"
                     /protein_id="HET7072702.1"
                     /translation="MSYLVAAPETLASTAGDVESIGSAIRSASASAAAPTTGLLAAAQ
                     DEVSAAIANLFGAYGQEYQAVVSRAAAFHNAFAQTLAAAGNAYAQADAAGAALVSFEL
                     AALGVPGQTPSGGGASGGSSLLTSVVRSASTDPTIALILGTTGTAQPGPDYLAAVNSS
                     FIQRLYPGAIPQGVFTPEQFWPVTPNLGNMTFGQSRALGVPLLNDAINTTLGGTTNHA
                     VVFGVSGSATLATDEINALMAAGSPYQGRLSFVLAGDPNNPVGGILERFPGFYIPFLD
                     VAFNGATPPNSPYPTVIYTAQYDGIADLPQYPLNVVSDLNAFMGYFFVHDTYGTPGTP
                     VFVTPDQVANAVPLPTSPGYTGNTQYYMIMTQNLPLLEPIRAIPYAGPPIADIFQPDL
                     RVLVDMGYPDYGPGGDYANIPTPAGLFVVPDPLTIIPDLALGAVQGPYGAAVEIGVEA
                     GLVSPSMFPTTYPWAPSLDPHLNFPIVQSSTTGLSTLTGAIGPYLELIPPVH"
     gene            22855..24300
                     /locus_tag="VFI55_01295"
     CDS             22855..24300
                     /locus_tag="VFI55_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PPE domain-containing protein"
                     /protein_id="HET7072703.1"
                     /translation="MSYLVTAPATLASTAADVARIGSAIDAANASAAGPTAGLAAAAE
                     DEVSAAIARLFSSYGREYRALIAQAVAFHDEFAQTLSAAGNAYAQAEATAATLLGQTS
                     TGSASTGIGALTSTVTPTSPDPVVALLMGGNTNPQPSPSYVGSVNVAYIQRLFPGAVP
                     QSLFTPEQFWPLTPSLGNLTFGQSVAQGVPLLNTAINNQINTLHNNVVVFGYSQSAVI
                     ATDEINALLALPAAQQPTAGQLAFILAGDPNNPVGGLLERFPGFYIPLLDVPFNGATP
                     QSPWHTTVYTLQYDGIADAPQYPLDVVSDLNAIMGFYYIHGTYPMLTTTQIASATLLP
                     TSGGNTQYYMVMTQNLPLLQPIREIPYVGTPFADLVQPDLRVVVDLGYADYGPDGNYA
                     NVPTPAGLFSVGDPFTIVPDLAIGAVQGVKGALVDVGWLPASDTPTTYPYVPSLDPHL
                     NFFLGQSSTTLLSTLSGDAGGVLELIPPVLN"
     gene            complement(24316..24945)
                     /locus_tag="VFI55_01300"
     CDS             complement(24316..24945)
                     /locus_tag="VFI55_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7072704.1"
                     /translation="MPSENGLTRREELLAVATKLFAARGYHGTRMDDVADVIGLNKAT
                     VYHYYASKSLILFDIYRQAAEGTLAAVHDDPSWTAREALYQYTVRLLTGIAANPERAA
                     VYFQEQPYIAEWFTSEQVAEVREKEAQVYEHVHGLIDRGISSGEFYECDSHVVALGYI
                     GMTLGSYRWLRPSGRRTAKEIAAEFSTALLRGLIRDETIREHSPLGPAS"
     gene            25108..25233
                     /locus_tag="VFI55_01305"
     CDS             25108..25233
                     /locus_tag="VFI55_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072705.1"
                     /translation="MDPSPDYDVSDEIEWFMRYVPWGLRGVLDGNGYPPPAYPPV"
     gene            complement(25238..26662)
                     /locus_tag="VFI55_01310"
     CDS             complement(25238..26662)
                     /locus_tag="VFI55_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HET7072706.1"
                     /translation="MNYVVTVLYIVAFSLFIYGLMGLTGPKTAVRGNLIAAVGMAIAV
                     GATFIKIAHTDQWVLIIAGLVVGVVLGVPPARLTKMTAMPQLVAFFNGVGGGTVALIA
                     LAEFIETKGFSAFQHGESPTVHIVVASLFAAIIGSISFWGSIIAFGKLQEILPGRPIG
                     LGKAQQVVNVLLLAAAITAAVIIGLGAHPGSGGVLLWWMIGLLGAAGVLGLMVVLPIG
                     GADMPVVISMLNAMTGLSAAAAGLALDNTAMIVAGMIVGASGSILTSLMAKAMNRSIP
                     AIVAGGFGGGGSAPADAGSGDKHVKATSAADAAIQMAYANQVIVVPGYGLAVAQAQHA
                     VKDMASLLEDKGVPVKYAIHPVAGRMPGHMNVLLAEAEVDYDAMKDMDDINDEFGRTD
                     VAIVIGANDVTNPAARNEKSSPIYGMPILNVDKARSVIVLKRSMNSGFAGIDNPLFYA
                     DGTTMLFGDAKKSVSEVAEELKAL"
     gene            complement(26659..27015)
                     /locus_tag="VFI55_01315"
     CDS             complement(26659..27015)
                     /locus_tag="VFI55_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HET7072707.1"
                     /translation="MYDELLANLAILVLSGFVGFAVISKVPNTLHTPLMSGTNAIHGI
                     VVLGALVVFGEVEHPSLAVQVILFVAVVFGTLNVIGGFIVTDRMLGMFKGKKKKALPA
                     AAERPALPAKTEKAAK"
     gene            complement(27018..>27870)
                     /locus_tag="VFI55_01320"
     CDS             complement(27018..>27870)
                     /locus_tag="VFI55_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HET7072708.1"
                     /translation="DEVGRLRRGQTLIGFLAPRNAENSIGALRAAGVQGFAVEAIPRI
                     SRAQVMDALSSQANVSGYKAVLLAASESTRFFPMLTTAAGTVKPASVLVLGVGVAGLQ
                     ALATAKRLGGRTTGYDVRPEVADQVRSVGAQWLDVGIDAAGEGGYARELTEEERAQQQ
                     QALEKAIAGFDVVITTALVPGRPAPRLVTAAAVEGMKPGSVVVDLAGETGGNCELTEP
                     GRTVVKHDVTIASPLNLPATMPEHASELYSKNITALLELLIKDGELAPDFDDEVVAES
                     CVTREGS"
BASE COUNT         4789 a         9588 c         8839 g         4654 t
ORIGIN      
        1 cccgacccga ttgactcatt cagcgacggg aggaacgcgc cgacctcggt cagccccttg
       61 gccaggtcct ctttgcgctg gaccaggatg tcgctgatcg tctgcaactg ctccagcacg
      121 tgcgtcaggt tcttgttctc cttgatcagc cccttgacct gctgcgaaaa cgcggcgacg
      181 ttgcccagca acgcgtcaat ggcctgcccg cgttcatcga aggcggccac cagcgtctgg
      241 gcgttgacca gcgcccggtc cacctgctcg ctgcggtcac ccagcacact ggccacctgg
      301 ttggcctggg cgagcaggtg tttgatctcc tcgtcgcgct tgccgatcgt gtcggagaac
      361 ttggccacac catccagcgt tgcgctcagg tgcgggtagg tctcatcgac ggtctgcgac
      421 agcacgtgca gcgactgctt gaccgtgtcg atgtcccagc cggtagcggc cttggtgacg
      481 tcgaagaacg cgtcgtagat ctggtaggga gtagtgcttt gccccagcgg caaggtggcg
      541 ccaggtcgta gcgtctcggc gccccgcggc tcgacctcga ggaccttctt acccagaatg
      601 gtgtcggtgc ggatcgccag ccggctctcg gtgccgatgg tttgggtgcc gagcgaaaac
      661 ttgaccaaga catggtcgtc ctggatcgtg atcccctcga tcctgccgac gtccatgccc
      721 gcaatgcgca ccttgtcgct attgatcagt ccgcccgaat cggtgaattg cccgtagtag
      781 ctgggcttgg cgaacaacat cggaacactg gtgatgcttt gaccaacgcc gatggccagc
      841 agcagcacca ggatgcccat gagtccgagg cgtagccggt tggggggttc tagcgttctc
      901 attgcggcga acacctacct gacggctggt tgaagagccg aaccgtgcgg accgggccac
      961 cgccctgcag cccgttgatc ttgagcgtga tgtcgcaggc gtagaagttg acgaagtcgc
     1021 cgtaggagcc gataccgcgc ccgatcaggt tcaacgcggt cgggatcttg tggagatagt
     1081 cgttgagttg gtcgtgctga tcgatcagcg gctgctgaac ggcgtccagg tagttgatgg
     1141 tcttgtgcag cagagcgcgg tcctcggcga gcaggtcggc caccgttccc gagacattgc
     1201 tgatgtgcgc ggtcccggcc gccagcgggt cggcgtggtc tttgagcccg gtgatcagca
     1261 cctcgaggtt gttgacggtc tgatcgaatt ccttgcgatg ccgcaccgtg gtgtccagca
     1321 ccacgttcaa gttcttgacc acctcgccga tcgcctgatc acgctcaccg atctgcgtgg
     1381 tcagctgcgc ggtgttgtcc agaatgtcgt tgatggtgcc gccctgaccc tgaaacaccg
     1441 tgatcagggc ggatgcgatg gcgttcactt tctgcgggtc cagcgcccga aacagcggct
     1501 tgaaaccacc gatcagcgcg tcgaggtcca gcgccggcga cgtgcgcgcc agcgggatga
     1561 aaccaccggg cggcaagatc cggtccgcgc cttcgccctc gccgcgcttg agctccaggt
     1621 agcggttgcc gatcaggtcc agatagcgga tctgcgcggt cgtcgactga tacagcggca
     1681 ccgagcggtc cacattgaaa tccacccgca cccgcttgcc gccgtcgatc aaatgcatcg
     1741 aggcgacctt gccgatctcc acccccgagg cccgcacgaa ctggccttgc cgcagaccgc
     1801 tgatattgct gaattccgcc gagtagccgg tggtccggtc aaaacgcagc tgaccgaaga
     1861 ccacgatgat catgaccgtg aagagcagca gcaccgtgga gacgatgccg agtcggatga
     1921 gggtcccggt gtttttcatg ggttgatcgt gttatcccct acctgacggc cccacacgta
     1981 ctcgatcgcg tagggggaac cggtgtcgag gtggttataa ggcgcaatgc tgttgccggt
     2041 gtccatcacc aactccggcg cgggccacag gtcatgggtg atgtgctgcc aacaacccgg
     2101 ggccccgccc ggtccgccgc gcgcgttcac ccgcggcagg ttctccggcc agatataggg
     2161 gttcggcgcc ccgccgacca caccggccaa cccgccgaac atcgatccga tggcggccac
     2221 cgcggccacc gggttcaaga acagccccaa ccccgacagc aactcggcgt ggtcattcag
     2281 cgaatagccg ttgccgccca ggtaggccgc ggccttgggc tcgatgtcgt gatagttgtg
     2341 gatcgtgcag tacaccgccg ggctgtaggt gtccagcagc tgcgcggtgg gcaccaggtc
     2401 ggcggctcca cgcgccagat acggcccgcc gcgctcgaag atgtcggcgc cggtgttacc
     2461 gaaccctgcc gccgacaaca acgcctggtc caaatccttt tgctgctggt tgatggtgcg
     2521 cgacgtgacc accgcgttgt tgaggaagtc gaacaaatcc ggcgaggcgt tggcatagac
     2581 gtcggccagg ttcgccaact gcgagaagtt gtggcggatc tgcggcatct gcgggttcac
     2641 gtcgtcgagg atcacgttgc tcttgaccag cgactccccg aacttgtcgc ccagcccggt
     2701 cagcgcttgg gcggcggcgc tcaacgtcag gttcagcttg accacgtcca ccttctccgc
     2761 gatggaggtg acggtctgga acagcgtgtt gagctctgtc gtcaccgatc tcgcgtcgat
     2821 gacgttctgc ggcgtgatcc tctgcggcga cgggttcttc ggtgcagtaa gcgacacgta
     2881 cttaccgccg aacagcgtgg aggtccggat gttggcatcc acgttggccg gaatcagctg
     2941 caggtagcgc ggatacacgt ccaaccggaa cttggcggcc ggcttgccgt cctggctcac
     3001 ctccgagatg gtggccaccc ggccgatctc caccccgttg taggtgacct tcgcccccgg
     3061 gtccatcacc aaacccgctc gcgtcgataa catcgtcaaa tgcgtcttgg gcgtgaaatt
     3121 tccccggaac tgcatataga ccaaccacag caccaggacg ctcacgacca ccagcaccag
     3181 gcccgcggcc ttgtacggcg gggtgcgggc tgcgtttacc ttccctgcgg gcgctgtcat
     3241 cgcggctaca ccgccagcgc gaagttgggg ttaacgccgt agatcgccag cgctatgagc
     3301 aggacgacga cattcaccga gaccaacgag aaccgcatcg atcgccccac cgcctcgccc
     3361 acgccgaccg gcccgccgcc ggcgttgtat ccgtagtagc aatgggtggt catcaccacc
     3421 accgagatga tgagcacttc gacgaacgac cagaacgcgt cgccgggccg gagaaacgtt
     3481 cggaaatagt ggtcataggt gcccgacgtc tgcccgtaca acaccgtcgt gactatctgc
     3541 ggagagatga acgacatcag catcgccagc gagtacagcg ggatgatcac cacgaacccg
     3601 gccacgatcc gcgttgacgc caggaaggcg atcgacttga tgcccatcac ttccagggcg
     3661 tcgatctcct cgctgatccg catggcgccc agttcggcgg tggcgccggc gccgacggtg
     3721 gccgccagcg cgataccgca aacaaccggc gcgacgaagc gcacgttgat cagtgcggcg
     3781 aagaacccgg tgaacacctc gataccgaga tagcccagcg acgcaaagcc ctggatggcg
     3841 accagcgagc tggcggacaa caccacgaat ccgacgatcg cgacggtgcc gccgacgacg
     3901 gccatcgcac cggtgcccat gccgatctgg gcgatcagcc gcagtgtctc cttgcggtag
     3961 cggcgcatcg cgaagggagt gtcggtaatg gcgacgacgt tgaaccagcc catctgcccc
     4021 agcgtgtcca gcgtccgggt cgtggccccg gcgtaacggt tgacggcggc ggaggcctgg
     4081 gggaagcggg cgcgaaatac ctgagccgtc gacatgtcag tgccccgttc cgaaccggat
     4141 accgatggtg gtcagcacga cgttcaccgc atacagcgcg accacacaca gcaccacggt
     4201 ctcgttgacg gcggtgccca ggcccttggc gccgccacgc acggtcagcc cgcggtaact
     4261 gccgacaagg ccggcgatga gtccgaacgt cgcggccttg acctcggcga tgaccacctc
     4321 aggcaggccc gtgatcagag tcagtgtgga caggtacgcg cccggcgata cgttctgcag
     4381 aaacacgccg aagatgaagc cacccaccag gccgactgtg atcaccagtg cgttgagcag
     4441 cgtggcgacg agggtcgcgg cgatcacccg gggcaccacc agccggtgga tggggtcgat
     4501 gccaaggact tccagggcgt cgatctcctc gcggatggtg cgagcgccca ggtccgcgca
     4561 gatggcggtc gacccggcgc cggcgaccac caacaccgtg acgatcgggc cgatctgggt
     4621 gaccgtgccg atcgccgcgc ccgccccgga gatgtcggaa gcgccgacct cgccgagcag
     4681 gatgttgagc gtgaagatga acatcacggt taccggtatc gagaccatga tcgtcggcag
     4741 gaacgcgacc cgggtgatga accagcactg ctcgagcagc tcgccccagt ggaagggaaa
     4801 acggaacagc gcctttccgg tgagcacgca catccggaag aacccgccga tcatggtcag
     4861 cggcgtctgc atttggtcgc ggaggtagct gcccagccgc ggctgggtcg ccgtcatcgt
     4921 cgcaccctca gggcagcacc acgtccgtcg caacgctgcg cgggatcgtg tcgcacggcc
     4981 cctccttgcc ccgaccctac gaatgtgacc atcgtctccc taccagcaag tagtaacgga
     5041 gaccacagga atgtacccga tggccgtgca cgtgtgaaca gcatccgcac aattagacct
     5101 tcgccaaaag cgagcaaacg ttacaggttt cgtgagcatt cctttctagt ggtaaaaccc
     5161 attgacaacg tgcgtccttc aagttagggg cgttccatag cgcaaacgca attcggtctt
     5221 gagaaccttt cccgccgggt tgcggggcag cgcatcgacg atctccagcg ccttggggtg
     5281 cttgtagcgc gccagccgct cggccagaaa ttcggccagg tcctcgagcc gcaggtcgtc
     5341 gccggtgacg gccgcgatcg ccaccggcac ctcaccccac ttctcgtggg cccggccgat
     5401 gacggcgacc tcgacgatgc gcggatggtc ggcgagcacg ttctccacct cggcgcagta
     5461 gatgttctcg ccgccggaga tgatcatgtc cttcttgcga tcgaccaccc acacgtagcc
     5521 gtcccggtcc atccggacga gatctccgga gtggaaccaa ccacccgcga acgcctcggc
     5581 cgtggccacg ggttggttcc agtagccgct catcaacgtg ggcgcccggt agacgatctc
     5641 gcccacctcg ccgatggcca cgtcgttcat gttttcgtcg accacccgcg cggcgaccgt
     5701 ggggaccacc ttcccgaccg atccacgctt gcggattgcg tcgtcgccga gcagcatgca
     5761 cgtcaccggt gacatctctg tctggccgaa cgcggccagc acctgggttc ccgggaacgt
     5821 cgccgacatc tcgcgcagca acgcgtcggg tgccggcgcg gccccccacg acatcacccg
     5881 cagcctcagc tcgcggggtc gcgcccgttg ttcggcgcag accgcctgcc attgcgccgg
     5941 aaccaagaag aggccggtga ccttctccgc ggccaacacg tcgagcagtt gcgcggggtc
     6001 gaagtcgccc agcggataga tcaccgtggg gagaccgagc aacatcccgg tcagcaggtt
     6061 gccgattccg gcgatgtgga agaacgggac gccgacgaaa ccgacgtcac tatctatatc
     6121 tgcgccgttg gtgtacagcc cggtcatcgt ctggccggtc aagttggcgt gggtgagcac
     6181 cgcgcctttg gcccggccgg tggtacccga cgtgtacatg atcagcgccg gcgactcgtc
     6241 cgggatgtcg acgggtccgt gggaatcgcc gggctcgcta atgaggtctt cgtagccaag
     6301 aaggccgtcc tcggtcgcgt ccccggccac gacgatcatt tcaagcaacg gctggattgc
     6361 tctgacgccg gtcgccaccg gggcaagcac cgattcggtg atcaccgccc gcgcttggca
     6421 gtcctccacg aggaaggcga tttcggcggg agtgagccgg aaattcagcg ggaccgcgat
     6481 ggccccgagc gagttgacgg ccagcaccga ctcgacgaat tcggtgcggt tgagcatcaa
     6541 gatcatgacc cggtcgccga agccgactct ccgacggctc aacgcatcgg ccagcgccac
     6601 gaccctgcga tgtaggccgt cccacgtgac ggttttcccc acaaaccgca gcgccgtccg
     6661 gttcggctgc atcagcgcgt gccgttcgag ttggttgacc cagttctgcc gacgggccag
     6721 gtacggctgc tccgtcagca gtgatgccag tgtcggatgg ctggccaatc gcgcggtcaa
     6781 ctcacgcctc ccttcactcg cgcaccgctt gcgtcacgtt gatatttgat aaaacatggt
     6841 gtggtgtggg tcacactatg gcgtcgacgc cccggtgaca agccctgtca aaacccgccg
     6901 gcccctatag accccctaag agccccgaaa gcccctgaca gctgacgtga acacgccgat
     6961 atcgatgagg ccgaagaccc agcggccact gcgccgggcg cagttgtccg acgaagtagc
     7021 gggccacttg cgggcggcaa tcatgtccgg gacattgcgc ccgggaacgt ttatccgtct
     7081 ggatgagacc gcagcggagc tcggggtcag cgtcacgccg gtgcgcgagg ctctgctgaa
     7141 attacgcggc gagggcatgg tgcagcttga gccgcaccgc ggtcacgtcg tgctgccgct
     7201 gacccggcag gacgtcgccg acatcttctg gctgcaggcg accatcgtca gggagctggc
     7261 cgccgccgcg accgatcaca tcaccgacgc cgagatcgac gagctcgagc gcatcaacga
     7321 tgcgctggcg acagccgtcg ggtccggtga cgccgaggcc actgcgggca acgagttcgc
     7381 gttccatcgc gccttcaacc aggccggcgg ccggatcaaa ttggcgtggt tcctgctgaa
     7441 cgcagcgcgc tacatgcccg tgctggtgta cgccgccgat ccgcggtggg gtgcggccgc
     7501 ggtcgacagt catcggcggc tgatcgccgc gctgcgtcgg cgcgacgctg ccgccgtgat
     7561 cgagcacacc gtctggcagt tcaccgatca ggcgaatcgg tcgaccgaga tgctggaccg
     7621 ggcgggcgtt ttcggttgac ccgggcccta gttgccggat agctgctcgg cgcgctgctt
     7681 cagggtttcc gcgagatggt cgagcacgtt gtggaggagc tgcttcacca ccggcgcggg
     7741 caccggcatg gtggtctcga cgtcaacgtc cacggtgagc aaactcgatg ccccggttcc
     7801 caccacgctg aacagttgtt cctgcttgga gaacaggtca ccttgctgca ggacggtctg
     7861 gatctggttt gcccccgggt agtagaccgc ctggatgaag gtgctgtgca tgccttggac
     7921 ctcggtgtcg agccgcagct ggctgggccg cccgtcgtcg tagcgggcga gcacccacag
     7981 gcccttgatc gactcgttcc attgcgggta cgcctcgaaa tcgccgacga tggccatgat
     8041 cgtcgcggca tcggcactga cctcgacggt cttgctcaaa actggcattg gcgaagcata
     8101 accgggcacc cggcggccct ccgcgctgtg gtcggcgctg cggctgcgcc gaaaccgacc
     8161 gcgccgggcg cgatttcagg ctagtggtcc acgcagactg tcgacagcag tgagcggatg
     8221 gcgtcgggaa ttgggaccgg cttgcggctg gcccgatcca cgtagacgtc cacccagtgc
     8281 cccaccgccg cgatcggttc cccgccggcc gtaggacccc cggagccgtt gccgggttgt
     8341 cggtggtcgg cgcgaaacac gccgagccgg taggtgacgc tgctgcggcc cagccgcgtc
     8401 accgagatgc ccaccgcgag ggtttccggg aaatggagtt cggacatgta gcgacaaccc
     8461 gactcggcga cgacgacgag ggcggacgcg gtcatcggat caacgctgac ggtcgtgttg
     8521 atccaggcgt tgatcgcggt atcgaagatc tcgtagtaga cggcgttgtt gaggtggccg
     8581 aacatgtcgt tgtcggccca ccgggttccc accggccaca gcagggggaa gtcgtcgctg
     8641 gtgagctcgt ccggcgcggg cgggaccgcg gggaccttcg gaaccgatgg acctgaagcc
     8701 atggctgtat tccagcatgt ccaacatccg gagggggcgt acccctgcac ggcggtagag
     8761 cagtgtggcc gtatttgtgg tgcagggcgc taatcgagcc ggatcagccc tgaaacgacc
     8821 ggtgagcggc catcacacag cagcgtctcg gtgggatcga ccgactgaca gccgatcccg
     8881 gccgccgcga gcgcataacc cttgaatttg cgggccgccg cgctgagcac gtgttgagcc
     8941 cgggtcatgg cgcgattgac gcccaacggc cacccttgag gcgctcggcc ccacaacgcg
     9001 acttcggtca accggtcgtc cagcgactcg agcaccttgg cgcgcacgcg cgcgtcggtg
     9061 gtgaaagcct cggcgctgac cgccaggctc tggctcgtcg gccccgtcaa ggcgaaccgt
     9121 gccgccggct ccgactcggg gtccagggcc tgcacaccga gaatccgcag cgagcgggtg
     9181 tcgcagctgt gctgcagcag caccgtcacc tcccagccgg ccattgcccg gtcgtagagc
     9241 cagccccccg ccgacgccac gacgtccgcg gcgctcgccg cgatgacgtc gagtcgatat
     9301 ttcagacact cgccctcagc gcgcgttgcc ggtccgacgg caatccggct gttctggctg
     9361 atgccgaacg ttgaagtgac catcaatccg ttcctcaccg ccgcgccgcg gggcgcgttg
     9421 gcgttaccct gcagcgcttg ggctccttgt tgtgagctgc cgccccacgc agcgccgatg
     9481 ccgggaccgg cccggtcgac gagtcgggtg actcatccga cagcgtgaca tttaagccag
     9541 aatttgtaaa gattcgacgg cccgatcacg acccggatcg ggccttgagt tcggcgagaa
     9601 cctcgtcggt gtgctgtccc agttgcggtg ccgcagaacg cggtgcccac ggtgtgccgt
     9661 ggaaatctgc gggcgtggcc accatcccga cgctgacctc gccgtccggc gcatcgacag
     9721 ggacgtccac caagcccccc gcggcgtaga actgctcgtc ggccacgacg tcctcgagtg
     9781 tgttgaccgg cgaccagaag aaatccggtt cgcccgcgaa gatctgcgcc cattcgtcga
     9841 gcgtgcgcgt tgcaaagatc tcgtccagct cggcaatgag ctgcaccgca ttcgcggcgc
     9901 ggtcccgggc gtcgcggaag cggggatcgt cgccccactc ggggtggccg acgacgcggc
     9961 acagcggggg ccagtgccga tcaccttgca gcccgacgat ccagaaacgc cgcccgtcgc
    10021 cggcggcata gttgttcatg cacgggttgc ccatcgattc gcgttgcccg atcgcgatcg
    10081 gctggccggt catcagatac gtgttgaggt cgaagctgac ggtgtagacg ccctgccggt
    10141 acagcgaagt ggtgaccagc tgacccgtcc cggtgcgttc gcgggcgagc agcgccgcac
    10201 agacggccgc ggccaaagtc atgccggccg agtggtcgcc catcccgccg cgctggaacg
    10261 ggggtgtatc gccgggccgg gtcagcagat gggccacacc ggcgcgggac cagaacgcgg
    10321 cgacgtcgta ggcggcacgg tcggcgtcgg gaccggtttc gccgtacccg gtgatcagcc
    10381 cgtagaccag gcgcgggttg cgggccgaca ccgacgcgaa gtccatcccc agacgtacca
    10441 acgcgccggg ccgcacattg gtcaggaaaa cgtctgcatc agagagcaat tcgaacgcgg
    10501 tgtcgcggcc cgctgcaccg gtgaggtcca agacgatgct gcgcttggag cggttgtcca
    10561 tttcgaacgg cgggttgacg ccgagatcgc agcccagcat ccggccgaac atgcgccccg
    10621 ggtcaccggt cggcggctcg atcttgatga cgtcggcgcc ccagtcggcc aggatgcccc
    10681 cggcagccgg tgcggccacc cagaccccga gctcgacgac cttgatgcct tgtaccggtc
    10741 cggccatggc tacgttccta ctccgcggtc ggaccgcccg ggcgtctggt cggcgtactt
    10801 cgggccactt acatgaaggg catcctcaac tcgctcgtcg ctgcggccgg cacggcgggg
    10861 ccggtaccgc ggagcgaggg cggcacgccc ggggcgggat tcgctcccgg cggcgagagc
    10921 ggcacgcctt gggcgggatt ggcccccggc aattggaacg gactgggcga gccgggattg
    10981 ccggccaggc cctgcaaagg gccttgtaaa gggccgggta tctggccgtt ggcgagcgag
    11041 ttcatgaagc ccgggcatcc catttgtatc gccatgctgg cggccaatcc gacgaggcct
    11101 ggcggaagtc ccgtgcttcc cgacagttgt gacgctatcg aggcgacggt cgcgcccggc
    11161 tgcacgagca tcgggcagat ggattgcccc attccggcga gggcgttgct cgtcgggccg
    11221 ttgttaccga agccgatgtt gttcggcggc acgcccgggg gcgggtcggc gtgcgccatc
    11281 ggcagcgacg ttgcccccgc ggtgatgatg ccgaccgcga ccaccagcgg acgaagcatc
    11341 gcgttgatcc gagccggcca tcctgcggtc gggcggatct ctgaggtgcg atgggccgtc
    11401 tgctcggccc acgcagggta cgtcatcggt gcagtcattt cattggcccc tcggcgataa
    11461 caggaataac aggaataaca gaaacagatg tgtactcatt tcactttggt aacgatggac
    11521 tacttaagtc acaggaacaa cacggttaga tcaaggtttg tgatctggcc gtttcctcac
    11581 cgttgtctca agaaacccca tgggacgctg cggggttcgg cgcacagccc gcctgcgggg
    11641 caccggttcg gccgtatcgt tggacctggc cgccattgaa ggcggaaagg atcaccatga
    11701 cgttagtagc cggacgtggt ccgctcagca acgatccggc cgggcggttc tcgcccccgc
    11761 tacccgccga cgccccctac atcggggtct acatcgagcc gcatccgcga cgcgtccagg
    11821 ctttccgcgg cgggcgcgcg gtgatcgata ccgagcaggc gctgatggtg caccggcgcg
    11881 gtcatccgct cagctacgtc ttcccggtcg acgaggttgg cgacgtgccc agcgagccgg
    11941 tcccggaggc cccgggctac gtccatgtgc cctgggacgc cgtcgacacc tggctggaag
    12001 aaggccgcca actcgtccac tatccgccga atccctacca ccgcgtcgac tgccgcccga
    12061 cgaagcgccg cctgcgcgtc gcggtcgccg gcaccacgct ggtggacacc gaagacacgg
    12121 tgatcctttt cgaaaccgcc ctcgaaccaa ggctttacgt cgatcccgcg cgggtgcgca
    12181 ccgatctgct gcaacgcacg aagacgacga gctactgcaa ctacaaaggc ttcgcgacgt
    12241 attgggccgt cacactgaac ggcctcgtca tcgatgacgc cgcctggagc tacgaggatc
    12301 cgttcccgga aagcctcgga atcaagggtt tcctcagctt cgacaccgaa cgcgtcgacg
    12361 tcgtcgccga actaccgctc acccgccgta gttgggcggc ccagcttgag ctcatgctgg
    12421 gcgatcatgt tgcggaagac ctccagggtg ccgccgtaga tccccagcgg taacgccagc
    12481 cggtaaaggt gttccgccgc accgtcaccg gcagagccgt gggcatcggt gggcagcgct
    12541 gatgccgttc ccaggatgtc catcatgtca ccggagatgt cgcgcatcgt ctgcgcgatc
    12601 gcgacgcggc cgaacatgcc gggcgtcgac agcgccgcct ccatccgggc gaagctgcgt
    12661 cccagccggt acttcaccga gtcgtcgtcc aactggcgcc tcccgtccgg gccggctcgg
    12721 ccagctatgg ccgcgacggt gtcgaccacc tcggccatca cgtcgccgtg gcctgccatt
    12781 gccgcgatgt gttgtaagcc atggtcttcc ggatcgacga tgccgtgctc ggaggccagc
    12841 gcggagcgca tcaccgtcca gccgccattc acctcaccga tgcggtagag gtcgtcgacg
    12901 cggacgtcgc tgtagtagac gatgttggtg cggtcgccgt cgagggtccg gacgccctga
    12961 atctcgatgc ccggggagtc gagaggcacc agaaacatgg tcagattctt gtgcttcgga
    13021 ccttcggggt cggtgttggt gagcagaaag acgtacctgg agttctgtgc gttcgaggtg
    13081 aacatcttgg agccgttgat gacccacgcc ccgtctgctg agccgtcgcg caccgcccgg
    13141 gtcttgcagg tggcgacatc cgaaccgccc tcgggttcgg tgtatcccag acacagccgg
    13201 atttcgccgg acagcacgcc cggtatcacc ttgccggcca gttccggtgc gccgaattgc
    13261 tgtaccagcc gggcaacggc ggacgtggtg ccccagtggt accacggcgt gtgtgccctg
    13321 gcgatttcga gtgcaaagat tcgccggcgc acggggctga acccgccttc ggactcctgc
    13381 ttccagtccg aggccaggta gcccgcctca cccagcgcca gatgtacgtc ctcgtcgaag
    13441 ttttcgccga actccctgtc gtggcgcagt acctcgtcgg tgacgtgctt gctgatgaac
    13501 gcccgggtct ggtcgagaaa atcctggtcc tcggcgcaga gttcgacgcg ggagaagtcc
    13561 atctagatcg cgtcgatcac gagggtgcgg aagcgttgca gcgtctccag cgcgtgcacc
    13621 atgctgtcac cgggcagccc gacgctgacc cacgtcactc ccagtgccgc aagcttttcc
    13681 aggccgtcca ggtaggcgtc ggcgttgaac tcgtcgttcg ccggactacc gccggcgaag
    13741 ttagcgaacg tgatgtcaat cgcggaccaa tcccggcccg ccgcatcgca tctgcgccgc
    13801 agatcgtcga tggtctcgcc gaggcgctgc accgagtcga tgaccgcggt gcccgccgtc
    13861 tgggcgagcc gggccggggc ggggaacggg caccagccgt cgccgtgctg cgccacccgc
    13921 tggcgcgagg ccgccgtgtt gccgccaatc cagatcggcg ggctgctgat cggtcgcggg
    13981 tgcgcggtga cgccgcgcgc gctgaaatgc tttccctcga acgatatgtc gtcgctggtc
    14041 cagatcgcct tgatgacctg cagcgcttcg tcgaacagct cggcgcgctc gtcgtagctc
    14101 acgcccagcg ccgcgaactc gcgcttgaga taccccaccc cgaccgcgag cgtgaagcgg
    14161 ccacccgaca gcaggtccag cgaggcgccg gacttggcca ccacgaacgg gtttcggtac
    14221 ggcagcacca cgatgttggg gatcagccgc agcgtggtgg tcctggccgc cgcgaaaccc
    14281 aacgcgacga agggatccag ggcgtcgtgt ccgccggcct ccaaccatcg ctgggagggc
    14341 gccgggtgat cggtgaagcc gaagccgtga atccccgccg cttcggcagc ggcagccacc
    14401 gtcgcaatgc cttccccgct cagcagctca gggttgtagg gatgggtgtg tatcgggtgg
    14461 gtgatggtga agtacatgtg gttttgcccc gcgctagaac cgtgcgcggg agtcgatcgc
    14521 gttgtcggcg gtgcgcagcg ggcggatcag cgcctcctgg gcaaaggaac acagcagttc
    14581 cccgtcctgc gcgtgcacgg tgccccgcac gtaggacatg cccgcgccga cctgggtgct
    14641 ctcgtgggtg tacagcagcc agccgtccca gcgcaccggt tcgtggaacg cgaccgagat
    14701 gctcatcggc gcggtcgaca ccgtgaggtg ggcctggctg gtgccgatgc cctcgtgcgc
    14761 ccgcatggtg gtggagatcc ccaggtgccc ggtgaagtac gcgaccagcg ccttggccag
    14821 atcgtcgcgg gtaggtatcg agtcgtagtg cagccacgcg tacagctccg gcggccccac
    14881 ctcgtcgggg ctgttgacgt cgacgacatc gacgagccgc agttggcgcc cggtcatcgg
    14941 catctcgcag tggtttgcat cggcgggcgc ggccacgtcc ggcagcggca gctggtggcg
    15001 gatgacgtcg ccggtcggga cgtcggccag caccgtgatg ctcaggcagc gccgcccgtg
    15061 ctggtgcacg gcgacgaccg cggtggcggt ggaccggccc tcggcaacga cgtcgagcac
    15121 gagttcgatc ggcggcccga cggcgacggc gcgggagaac accgcgtgcg ccgagcgcac
    15181 cgacttgtcc gggaaccgtt tcgccgcggc gacaatcgcc tgcgcaagca cctgggtgcc
    15241 ctccaccacc tgccggccgt cctggccggc cccgacgtca ccgccggtgt tgccgacgaa
    15301 ccggtcgggc ccgtcggccc gcacgtcaaa taggcccaac aggctctcga cggaccactg
    15361 cgcctgctcg gattcgcggg aaatattggt caaatccgcc ccagcctctc gccatcagcc
    15421 cagggcaaag cgtgacactt tgtgtacagt ttgtaaagca aacccgcagg acgcgccgga
    15481 ttgacaccgg gacccgcggt gcgtgacact tttgcggtgt tttgtaaagg cgcgagcgaa
    15541 tgacacccga accactggcc aacgggttct gcttcggcga ggggccacgc tggttcgagg
    15601 ggctgctgtg gttctcggac aggctgggtg cggcagtaca caccgcggac atgcgtggca
    15661 cgctgaccac gttgccgctg ccggggcact ccccttcggg tctggggttt cggcccgacg
    15721 gttcgctgct catcgcctcg accgaagacc ggcaggtgtt gcggtatgac ggcgaatgcg
    15781 tggtcggcat cgccgatctc accggcctgg cgccggccca cctcggcgac atggtcgtcg
    15841 acgacgaagg acgtgcctac atcgggtccc aggccttctc ggctggcgtg atcatccggc
    15901 tcgaccccga cgacaccgcc accgtcgtcg ccgaagacct cgactttccc aacggcatgg
    15961 tcatcacgcc ggaccgcacc acgctgatcg tcgccgagtc gaccggccgg cggctgaccg
    16021 cgttcagcat cggcgagggc ggcgcgctcg gtgaacgccg ggtcttcgcc gacggcctcg
    16081 acgggccgcc cgacggtatc gcgctcgatg ccgacggtgg cgtgtggaca tcgatgacgc
    16141 tggctcacca attcgcgcgg atcgtcgaag gcggcggcgt gacggatcgc atcgacatgg
    16201 gcgagcgggt tgccatcgcc tgcgcgctcg gcggcccgac acgccgcaca ctgtttctgc
    16261 tgtcgagcac cgacgcgtat ccgcagcatc tggtcggcac ccggctatcg cgactggacg
    16321 ccgtgcaggt cggcagcccc ggcgccggcc tgccctgaga catcccgaga ggttgacgag
    16381 tgacggactc ctactacgag ctgatcgatg gcactgacgc gcaaggagag aagttcaggg
    16441 cgaccgacct ggcccgcggc acctggtcgg ccgcgataca gcacggcgga ccggtgtcgg
    16501 cgctactggt ccgggcgctg gaacggtgcg agcggcgcga cgacacccgg ctgtccaggg
    16561 tcgtcatcga cctgctgggc ggagtgccgg ccgacggcga cctgtgggtc agctcgcaac
    16621 tgctccgcgg cggcaaacag atcgagctgg tcggcgccga gatcctggcc cccggtcccg
    16681 atggcgatcc ccggccggtc gcgcgcgcca gcgggtggcg cttgcagcag caggacaccc
    16741 ggtcgctgac gcacgccgcg gcacccctgc cccggccgag gaccgaggcg agcagccgca
    16801 acatgaaggc actgatgtgg gaccgcaatt acgtgcacag cctggactgg ctgtggctga
    16861 ccgaaccgct gaccgctggc ccgggagaat cctggatcac tccgaccgtt gacctggtcg
    16921 gcggcgaggc catgacaccg ctggaacggc tcttcgcggt ggccgactgc gccaacggca
    16981 tcggcagcaa gctgaacatc accaagtgga cgttcctcaa catcgacctg gccgtgcacg
    17041 tgttccgcat ccccgacggc gaatggatcg ggattcgcgc cgagaccagc tacgggccgg
    17101 acggcgtcgg tacgacaatt ggcacgctgt ttgacgaaca gggcgcggtc ggcgccatcc
    17161 agcagtcggt gctggtgcgc cggcgcccgg gccgatcctg aggtgggagc gacagacatg
    17221 agccaggcac ccccggctga cacaaccgtg accgacacct cgacgcgtca gcggatcctc
    17281 gcggccaccg ccgaggtcct gggccgcaac ggcaaaacca agctcagcct ctcggacgtg
    17341 gccaaccagg ccggggtctc gcggccgacg ctgtaccgct ggttcgcgtc caaggaagag
    17401 ctgctgtccg cgttctcgcg ctacgagcga caggtcttcg aaagcggcct ggccaaggcg
    17461 accgccgggc tgaaggggac ggacaggctc gacgccgcgc tgcgattcat cgtcgaatac
    17521 cagcactcgt attcgggtgt gcggatggtc gatgtcgagc ccgagcacgt catcgctcag
    17581 gtgtcccgcg ccatcccgca gatgcgggag ggactcgaac ggctgcttcc cgggcctaac
    17641 gccgcggtga aagcggctac ggtgatccgg atcgccatct cgcactacat cgttcgcagc
    17701 gacgacgccg cccagtttct ggcgcagctg cggcacgccg tcgggatcaa ggctccggcc
    17761 gaatagcccg cgccggccgg cgcgcgcaat tgcgcaccct cgcgacgggt cagtcgaaca
    17821 gcaccgcggc attcagataa ccggtcgggt ccaacgcggc cttgaccgtg cgcatcgctg
    17881 cgatatcggc ggcctcccgc gacatcccga gataggcgcg cttgcggctg ccgacgccgt
    17941 gctcggagct gacgttgccg ccgctcccgg cgatgaggtc catcatcggc gcgtacagct
    18001 cgcgctcacg ctcgagcggc acccgcagca cgttgaggtg caagttgccc tccccgatgt
    18061 ggccgaacag cagccgcagc gcgtcgtgag cgcgcgcctc gatcaacgcc gttgcgtcgc
    18121 gggcgaattc ggcgatcgcc gccagcggca acgagacgtc gaacttcagc ggcggcccgt
    18181 acacgccgag cacctcggcg agcgattcgc gcaggcgcca caacctttgc tgcgccgcga
    18241 cgtccacgcc gacggcgggc tcgccgcaca gctgcaggcc gtcgagcagt tcgccgagcc
    18301 gatcggtctg gtcctggtcg gccgccagct ccaccaacag cagccagtcg ccggctaccg
    18361 gagcgcccac ccccagatgc tcggcggtca gcgccgcggc ccggccgtcg atcagctcca
    18421 gcgccgcaat cccgtccacg tcgcgaaatg tccggccggc gtcgaccagc gcgtcgaggt
    18481 cggcgaaccc gcagaccgcc gtgatccggt gcgatggcgt ggggtgcagc cgcagatcca
    18541 gcgacgtgat gacgccgagg gtgccttcgg caccgacgaa caacgccggc aggtcgtagc
    18601 cggtgttgtc gcggcgcacc aggctgtgcc ggcgcatcag cgaaccgtcc ggcagtgcca
    18661 cctgtaagcc gacgacttgc tcgcccatgt tgccgtagcg gacggtgcgc agcccgccgg
    18721 cgtgggtcga ggccatgccg ccgacggtcg ccgtgtcgcg tgcggccagg tccacgccga
    18781 acaccaaacc cgccgccgcc gcagcgagtt gcaccgcggc cagcgtggcc ccggccccgg
    18841 ccgcgacgcg gcgctcgacg acgtcgacgt cgcccaccgc gctgagccgc tcggtggaca
    18901 gcagcacgtc ttcgtactcg ggaacggtgc cggccaccag cgaggtgcga ccgccctgca
    18961 ccgtcacgtg cgccccggcg tcccggcaca cccgcagcac ctccgcaacc tgctcgggag
    19021 agcccggccg caccagcgcg ctggcccgtc cgcgatagcg gccggtgtgg tcgacgctgc
    19081 ggccggccag cacgtcgggg tcggtgatca catgagttga tccgacgatg ttggccaggt
    19141 ggttggccag ttggtcggcc atgctgcgcg gcatgctcgc ggttatagca ccgcttgccg
    19201 aacccagccc tcagagctgt ttgtgcggca cgtcggacac cagtcccccg tccacgacga
    19261 actcgctgcc cgtcgcgtac gaggactcgt cgctggcgag aaacaccaca aacatcgcga
    19321 cttcgcgagc ctcgccaggg cgacccaacg ggacggtgac catgtcgtcc ggcagcttgg
    19381 cggtcatcgg ggtgcggatg aagccggggt ggatcgagtt cacccgaatg ttcagcggcg
    19441 ccaattccag ggccgccgac ttggccaggc cgcgcaccgc ccatttcgac gccacgtagg
    19501 ggtgcacggc cggggcgccg cgcaacccct cgatcgacga cacattgatg atcgagccgc
    19561 cgcctgcggc ggtcatcggc tcgacggcca cccgcatgcc caggaaggtg ccggtcaggt
    19621 tgacgtcaat caccttctgc cacttggcca aatcgaagtt cttcagctgc ccgagcgcca
    19681 cgatgccagc gttgttgacc agcacgttga gtttcccgaa ttcgccggtg gccgttgcga
    19741 ccgccgcgtc ccactggtcg ggccgtgtca cgtcgaggtg cacatagcgt gcggcgtcac
    19801 cgatctcgtc ggcaagcgcc ttgccctcgt tgtccaggat gtcgccgatc accaccttgg
    19861 cgccttctgc cacgagcaga cgcgcgtctt cggcgcccat tccgcgggcg cccccactga
    19921 tcagcgcaac tttgcctgct acccgtccca cgcgggtctc ctttcggttc aagtggccac
    19981 caaactgttt ggcgcgaaaa ggccctttcc gctgaccagc ggaaggtcaa gcgtggtccg
    20041 gatgcccggc ggcgcggcga tcaccgccgg gatggcgttg acgatgcgcc cggccgcgcc
    20101 gacgatcgcg gcgtggttgt gatcgccctt gcggctgctc ggcacgatgt ccacggcata
    20161 ggacggttcc ccgacgatct cgacgcgata cgagccgccg cccgaagccg gttgcggcca
    20221 gtcgggtcgc aggtcgggcc gcaaccgggt gatgtgctcg atgacgatgg cgggacgccc
    20281 gttggacatg ccgcggatct cgaaccgcag cgcggccatc gtgccctccg ccacgcgccc
    20341 cacggcgatg tcgaaggcct ccggcgccgg ctcacgctga tacgactcgg tgatcccgtc
    20401 gagctcgatg ccgagcccgg ccgccagctg acggatcgcg ccgccccacg cgatgctgag
    20461 cacccccggc agcagcagca tgggcacgtc gtccagcggc cgaccgaatc ccatgtagtg
    20521 catgacctcg gccccgtcgt aggtcgcgta gtcggcgatc tccatgcagc gcacctgctc
    20581 gatgcgctga caggtccccg aaagcgcgaa cggaatgagg tcgttgatga atccggggtc
    20641 cacgccgttg atgaagatgc tcgaattacc ttgctgcgca gcgtcttcga cgcgggcgat
    20701 gtacttgtcc ggcagcacac cccagggaaa ctgcagcatc cccggcgacg accccacgac
    20761 gttgacaccc gcggccagga tgcgcatgac gtccttcatc gcatcgggca gccgggtatc
    20821 gcccatggcg cagtagacga cacactcggg accggtggcg agcacggcat cgagatcgtc
    20881 gacggccgcg acgcccgtaa cggcggcggc gccgcacagc gcgcccgcat cctttcccac
    20941 cttctccggc gtggacacac acaacccggt cagtttgaac cgcggactgg tgacgagccc
    21001 ggctagggcg agccttccga catttccggt accaacgtgg gcgacacgga tagccatgag
    21061 agtctccgtc cctcgaacgc tcaagaactt ttctagacac tagtccacaa tttttggaag
    21121 gcccaccgcg aagcgtgggc cctaacagga caaacttgtg caaaacgaca gacttccggg
    21181 cagcacagct ctactctctg tcaataccta agacccacgg ttgcgggtaa gtcccggtct
    21241 ctgggaagga cagggagatg tcgtatctgg tagctgcccc tgaaactctg gcgtcaacgg
    21301 ccggcgatgt ggaaagcatt ggttcggcga ttcgctcagc cagtgcgagc gcggcggccc
    21361 cgacgaccgg actgctggcg gcggcgcagg atgaggtgtc ggcagcgatc gcgaatctct
    21421 ttggcgcata cggccaggag taccaagcgg tcgtctctcg ggccgcggcc ttccacaacg
    21481 cgttcgccca gacgctggcc gccgcgggca acgcctacgc gcaggccgac gcggccggcg
    21541 cagcgctggt ctcgttcgaa ctcgccgcgc tgggcgtgcc cggccaaacg cccagcggcg
    21601 gcggcgcgtc cggcggcagc agcctgctga cttcggtggt gaggtcggcc tccacggacc
    21661 caacaattgc cttgatcctc gggaccacgg gcacggcgca acctggcccc gactacttgg
    21721 cggcagtcaa cagctcgttc atccaacgcc tctacccggg ggccatcccc cagggcgtgt
    21781 tcacgcccga gcagttctgg ccagttaccc caaacctagg caatatgacg ttcggccagt
    21841 cccgcgcctt gggcgtcccc ttactgaacg acgcaatcaa cacgacactc ggcggcacga
    21901 caaatcacgc agtggttttc ggcgtctcgg ggagcgccac gcttgccacc gatgaaatca
    21961 acgccctcat ggcagcgggc tcgccgtatc aaggccggct gtccttcgtg ctggctggtg
    22021 atcccaacaa ccccgtcggc ggcatcctcg agcgcttccc aggtttctac atcccgttcc
    22081 tggatgtggc attcaacggc gcgactccgc cgaattcgcc ctacccgacc gtcatttaca
    22141 cggcccagta cgacggtatc gccgacctcc cgcaataccc gctcaatgtc gtctcggacc
    22201 tcaacgcctt catgggctac ttcttcgtgc acgatacgta cggaacccca ggaacgcctg
    22261 tttttgtgac gcccgatcag gttgccaacg ctgtcccgtt gccgacctct cccggctaca
    22321 ccggcaacac ccagtactac atgatcatga cgcagaacct gccgctgctg gagccgattc
    22381 gcgccatccc ctatgccggt ccgccgatcg ccgacatatt ccagcccgat ctgcgggtgc
    22441 tcgtcgacat gggctacccc gactacggac ctggcggcga ttatgcgaac attcccaccc
    22501 cggccgggct gttcgttgtg cccgatccgt tgaccatcat ccccgacctg gccttgggtg
    22561 ccgtgcaggg accgtatggt gccgcggtcg agatcggggt ggaagccggg ttggtgtcac
    22621 cgtcgatgtt cccgaccacc tacccgtggg ccccgtcgtt agacccgcac ctgaatttcc
    22681 ctatcgtcca gtcgagcacg acggggctgt ccacgttgac cggggccatc gggccctacc
    22741 tggaattgat tccgccggtc cattaggccg gggcacgcca cccattctcg ggtccgggca
    22801 gacgatgccg gcggaccggc acgtcgggat cgaccggggc agagatagga ggagatgtcg
    22861 tacttagtca ctgcccctgc gacgttggct tcgactgccg ccgacgtggc acgcatcggc
    22921 tcggcgatcg acgcggccaa cgccagcgca gcaggcccga ccgccggttt ggcagcggcg
    22981 gccgaagatg aggtgtcggc ggcgattgcg cggttgttca gctcgtacgg ccgggaatac
    23041 cgggcgctca tcgcccaggc tgtggccttc catgacgagt tcgcccaaac gctttccgcc
    23101 gccggcaatg cctacgccca ggccgaggcc accgccgcca cgctgctggg acaaacgtcg
    23161 acgggcagcg cgtccaccgg catcggcgcg ctgacgtcga cggtgacgcc gacctccccc
    23221 gaccccgtgg tggcactgct catgggcggg aacaccaacc cgcaacccag cccgtcctac
    23281 gtggggagtg tcaacgtcgc ctacatccag cgcctcttcc ccggagccgt cccgcaaagc
    23341 ctgttcacgc ccgagcagtt ctggccgctg acccccagcc ttggcaattt gaccttcggc
    23401 caatccgttg cgcagggcgt gcccctgctg aacaccgcga tcaacaacca aatcaacacc
    23461 ctgcacaaca acgtggtggt tttcggctac tcccaaagcg ccgtgatcgc caccgacgaa
    23521 atcaacgcgc tactggcgtt gcccgccgcc caacaaccca ctgccggcca gctcgccttc
    23581 attctggccg gcgacccgaa caaccccgtc ggcggtctgc tcgagcggtt tccgggcttt
    23641 tacatcccgc tgctggacgt gccattcaac ggcgcgactc cgcaatctcc ctggcacaca
    23701 acggtttaca cgcttcaata cgacggcatc gccgatgctc cgcagtaccc gctcgacgtg
    23761 gtgtcggacc tcaacgccat catgggcttt tactacatac acggtacgta ccccatgctc
    23821 acgacgaccc agattgccag cgccacgctg ctgccgactt cgggcggcaa cacccagtac
    23881 tacatggtca tgacgcagaa tctgccgctg ctgcagccga ttcgtgaaat cccttatgtc
    23941 ggaaccccgt tcgccgacct ggtccagccg gacctgcggg tggtcgtcga cctgggctac
    24001 gcggactacg ggcccgacgg caactacgcg aacgtgccca ccccggccgg gctgttctcc
    24061 gtaggtgacc cgttcacgat cgtccccgac ctggccatcg gggccgtgca gggagtcaag
    24121 ggtgccctcg tggatgtcgg ctggctgccg gcatcggaca cgccgactac ctacccgtac
    24181 gttccatccc tggatccgca cctgaatttc ttcctcggcc agtcgagcac gacgttgctg
    24241 tccaccctca gcggcgacgc cgggggtgtc ctggaattga ttccgccggt tctgaattga
    24301 cccgcgggga cccgctcagc tcgcgggtcc gagcggggaa tgctcgcgga tggtctcgtc
    24361 gcggatcaag ccccgcagca gcgcggtgct gaactcggcg gcgatctcct tcgcggtgcg
    24421 ccgcccgctt ggccgcagcc agcggtagct gcccagcgtc atcccgatgt agcccagggc
    24481 caccacgtgc gaatcgcatt catagaactc gccgctggag atgccgcggt cgatgagccc
    24541 gtgcacgtgc tcatagactt gggcctcctt ctcgcggact tcggcaacct gttcgctggt
    24601 gaaccactcg gcgatgtagg gctgctcctg gaagtacacg gcggcgcgct cgggattagc
    24661 cgcgatccca gtaagcaggc gaacggtgta ctggtacagc gcttcccgcg cggtccacga
    24721 tggatcgtcg tgcacggcgg ccagcgtgcc ctcggcggcc tgccggtaga tgtcgaacag
    24781 gatcagcgac ttgctggcgt agtagtggta gacggtcgcc ttgttcagtc cgatcacatc
    24841 ggctacgtcg tccatccggg taccgtgata gccgcgcgcg gcgaacagct tggtcgcaac
    24901 ggccagcaat tcctcgcggc gcgtcagccc gttctcggat ggcatctata ctcactatct
    24961 tcgctgccgg cagggcgggt gggccccgaa ccaggctaat tatcaatcaa ctggttggac
    25021 agtttaggca tgatggcggg tgtcgcagcg actcggatgg gactacactc gtttgacgac
    25081 cgaagtcgcg accagatggg tggaccaatg gatccgagtc cggactacga cgtctccgac
    25141 gaaatcgaat ggttcatgcg ctacgtcccg tggggcctgc gtggcgtcct cgacggcaac
    25201 ggctatccgc caccggctta tccgcccgtc tagagaccta taacgccttc agttcctcgg
    25261 cgacctccga cacggatttt tttgcgtcgc cgaacaacat cgtcgtcccg tcggcataga
    25321 acagtgggtt gtcgatgccg gcgaatccgg aattcatcga tcgcttgagg acgatgacgg
    25381 agcgggcttt gtcgacattc aggatgggca tgccgtagat ggggctggat ttttcgttgc
    25441 gggctgcggg gttggtgacg tcgttggcgc cgatcacgat ggcgacgtcg gtgcgaccga
    25501 attcgtcgtt gatgtcgtcc atgtctttca tcgcgtcgta gtcgacttcg gcttcggcca
    25561 gcaacacgtt catgtgcccg ggcatccgcc cggcgacggg gtggatggcg tatttgaccg
    25621 gcaccccttt gtcttccagc agggaggcca tgtccttgac ggcgtgctgg gcctgggcca
    25681 cggccagccc gtagccgggc accacgatca cctggttggc ataagccatc tggatcgcgg
    25741 catccgcagc cgaggtggcc ttgacatgct tatcgccgct accggcgtcg gccggggcgg
    25801 acccgccgcc accgaaccca ccggccacga tcgcgggaat ggagcggttc atcgccttgg
    25861 ccatcaggct ggtcaaaatc gaccccgagg cgcccacgat catcccggcc acgatcatcg
    25921 cggtgttatc caacgccaac cccgccgcgg ccgccgagag cccggtcatc gcgttgagca
    25981 tcgagatgac caccggcatg tccgccccgc cgatcggcaa caccaccatc aaccccaaca
    26041 ccccggccgc gcccagcagc ccgatcatcc accacaacaa caccccaccg ctgccgggat
    26101 gggcgcccag cccgatgatc accgccgcgg tgatcgcggc ggccaacagc agcacgttga
    26161 ccacctgctg cgccttgccc agcccgatcg ggcggcccgg caggatttcc tgcaacttgc
    26221 caaacgcaat gatcgacccc caaaacgaga tcgacccgat aatcgcggcg aacaacgagg
    26281 ccaccacgat gtgcacggtc ggcgactcgc catgctgaaa cgccgaaaac cctttggttt
    26341 cgatgaactc cgccagcgcg atcaacgcca ccgtgccccc acccaccccg ttgaaaaacg
    26401 ccaccaactg cggcatcgcg gtcatcttcg tcaaccgcgc cggcggcacc cccaacacca
    26461 cccccaccac cagcccggcg atgatcaaca cccactggtc ggtgtgggcg atcttgatga
    26521 aggtggcgcc caccgcgatc gccataccca ccgcggcgat caaattgccc cgcaccgcgg
    26581 tcttgggccc ggtcaacccc atcaacccgt aaatgaacag cgaaaacgcg acaatgtaga
    26641 ggaccgtcac cacatagttc atttggcggc cttttcggtc ttggcgggca gcgcgggccg
    26701 ctcagcggca gccggcagcg ccttcttctt tttgcccttg aacatgccca gcatccggtc
    26761 ggtcacgatg aacccgccga tcacattcaa cgtgccgaac accaccgcca cgaacaaaat
    26821 gacctgcacc gccaacgacg gatgctccac ctccccgaac accaccaacg cacccaacac
    26881 cacaatgccg tgaatcgcgt tggtgcccga catcaacggg gtatgcagcg tattgggcac
    26941 cttggaaatc accgcgaacc caacaaaccc cgacaacacc aaaatcgcca gattggccag
    27001 cagctcgtca tacatctcta ggagccttcc cgtgtcacgc acgattccgc gacgacttcg
    27061 tcgtcaaagt ccggcgccag ctcgccgtct ttgatcagca gttccagtag cgcggtgatg
    27121 ttcttgctgt acaactcgct ggcatgctca ggcatcgtcg ccggcaaatt caacggcgac
    27181 gcgatcgtca catcatgctt gaccaccgtg cgccccggct cggtcaactc acaattgccg
    27241 ccggtctccc ccgccaaatc caccaccaca ctgcccggct tcatcccctc caccgccgcc
    27301 gcggtcacca accggggggc cgggcggccc ggcaccagcg ccgtggtgat caccacatca
    27361 aacccggcga tcgctttttc caacgcctgc tgctgctgag cgcgctcctc ctcggtcagc
    27421 tcacgggcat acccaccctc acccgccgca tcaatcccca catccagcca ctgcgccccc
    27481 accgaacgca cctgatcggc cacctccgga cgcacgtcat accccgtcgt gcgcccgccc
    27541 aaccgcttcg cggtcgccaa cgcctgcaac ccggccaccc ccacccccaa caccaacacc
    27601 gacgccggct tcaccgtgcc cgccgccgtg gtcaacatcg ggaaaaaccg cgtcgactcc
    27661 gacgccgcca acaacaccgc cttatacccc gacacattgg cctgcgacga caacgcatcc
    27721 atcacctgcg cccgcgaaat ccgcggaatc gcctccaccg caaacccctg cacccccgcc
    27781 gccctaagcg caccgatcga attctccgca ttacgcggcg ccaaaaaacc gatcaacgtc
    27841 tgcccccgcc gcaacctccc cacctcatct
//
LOCUS       DASSGQ010000031         8806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1278229,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000031 DASSGQ010000000
VERSION     DASSGQ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8806
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278229"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..2562
                     /locus_tag="VFI55_01325"
     CDS             160..2562
                     /locus_tag="VFI55_01325"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET7072709.1"
                     /translation="MVDGMLAAVRVLDLSSGNADPVTRLFADLGADVLKVEPPGGNLG
                     RDELPTLAGASIPFAVHNANKRSAVLDPFDENDCRLFLDLAAYADIVVDSGIDGLADA
                     FGAPGVDLATRYPHLVVLSITDFGATGPRSSWRATDPVLYAMGGSLSRSGPTTGTPVL
                     PPDGIASATAAVQAAWAALAAYYNRLRCGTGDYIDFSRFDAVVMALDPAFGAHGQAAA
                     GIRGTPQWRGRPKNQDAYPIYPCRDGYVRLCVLAPRQWRGLRRWLGEPQEFQDPKYDA
                     IGARFAAWPEISVLIRALSAEHTMKELVAAGQAHGVPIAAVLAPSQILGSEHFQAVGA
                     ITDAELVPGVRTSVPTGYFVVDGQRAGFRTPAPTAGQDQPYWLANPPAVPSPAGRQGG
                     YPFAGLRILDLGVIVAGGELSRLFGDLGAEVIKIESADYPDGLRQARAGDAMSESFAW
                     THRNQLGLGLDLRSTEGARIFGRLVAQADAVFANFKPGTLASLGFSYDALRAINPRIV
                     LAESSAFGDRGPWSARLGYGPLVRAATGVTRLWTSDDPDGRSDTARHAFYDATTIFPD
                     HVVGRVTAFAALAALIHRDRTGRGAHVHISQAEAVVNQLDTLYVTQAALEAGISRIRS
                     DTSLHAVCPCAGDDEWCVISIQSDNEWRSAASVFDHPAWADDPRFSSAESRLANRHEL
                     VGLVSAWTRMRTPVRAAELLQAAGIAAGPMNRPPDLLEDPQLAGRKVYCDMVHPLFDH
                     PLPAETGPAPFQHIPPAPQHPAPLPGQDTREVCRNLLGMTADETERLIHDRVLFEPTG
                     GA"
     gene            2605..4056
                     /locus_tag="VFI55_01330"
     CDS             2605..4056
                     /locus_tag="VFI55_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HET7072710.1"
                     /translation="MTATSVPTELSARYFIGGRFFTADNVQPVVEAATGEPLGDGACV
                     TESDIDDAVAAARQALPEWRSSSPDHRAGLLNALAAALKSRAKATSQLVSRENGMPIS
                     LSRGANGAFPPLLLRYYAQLVTEIPIEEIRPGVTGHTIVRREAVGVVAAIIPWNYPQA
                     LAVMKIAPALAAGCTVVLKASPETALDALVFADAAVEAGLPPGVLNVVSGGPATGAYL
                     VAHPGVDKVAFTGSTATGRVVAEMCGRLMRPVTLELGGKSAAIILDDADLDVTMRGLR
                     TASFVNNGQTCHLSSRILAPRSRYDEIVDAVAALAESMTVGDPLNPSTEIGPLVSSRQ
                     RDRVLSYIAVGKDGDARLVAGGSVPKDQPRGWFVSPTVFADVDNSDRLAREEIFGPVL
                     TISAYDTDDQAIALANDSEFGLAGTVWSADTERATTVARAVHTGSIGVNDYALDVRAP
                     FGGVKASGLGRELGPEGLEAYRTLKSIYRTGSA"
     gene            4176..5660
                     /locus_tag="VFI55_01335"
     CDS             4176..5660
                     /locus_tag="VFI55_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HET7072711.1"
                     /translation="MTVDPRTPVLIGYGQVNHRDEIDPATRSVEPVDLMAAAARRAAD
                     SRVIEAVDSIRVVNVLSAHYRDPGRLLGDRIGATGFTTLYSPVGGNVPQSLVNQACLD
                     IQRGRANVILLAGAETWRTRTGLKAKGSKLTWTVQDESVPMAEVSGTDVPMAGAAELR
                     IHLNRPAHVYPLFEQALRIANGESVKDHLNRVAELWARFNAVAVSNPHAWIRKPVTAE
                     EIRRPGPQNRMISWPYTKLMNSNNMVDQGAALVLTSVERARGLRIPAERWVYPHAGTD
                     AHDTPAVADRDELHRSTAIRIAGRRALELAGLHIDDVDYVDLYSCFPSAVQVAAAELG
                     LSVEDSARPLTVTGGLTFAGGPWSNYVMHSIATMAELLVANPGRRALITANGGYLTKH
                     SFGVYSTEPPAEFRWEDMQPAVDREHTREGLVEWEGVGTVEAWTTPFNREGQPERAFL
                     AVRTPDGSRALAVIADPTAAETTVREDIGGAKVAVAADGSAALL"
     gene            5770..7875
                     /locus_tag="VFI55_01340"
     CDS             5770..7875
                     /locus_tag="VFI55_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7072712.1"
                     /translation="MQILVTDATGAVGRLVAQQLIAAGHTVSGIAEFPHPYLDPDVEF
                     VCASLTDPALQDLSDEADVVIHLAPVDTTAPGGVGIDGVAHVTHAAARAGARLLFVSQ
                     AAGRPDLYRPAEELVSTSWAPSLVVRIAPPVGRQLDWMVCRTVATLLRSKVSAQPMRV
                     LHIDDLVRFLVMALNTDRTGVVDLATPDTTNVITAWRMLRSASPRSRLRGVRSWAQLI
                     PEMDIAAAQEDWAFEFGWQALEAVADTARGLVGRRLEDTGATSRGGQLALPVEVAPRP
                     LSSDELHRAAPEGMEAEFDDRIDARFPVFSAARLAEALPGPLTPITLDVQLSGLRAAS
                     RVMGQVLALGGVVDDEWGSRAIAVFGHRAYIGVSANVVAASQLPGWDQDAVARHALVD
                     QPQVGDVLPFGEPRLAEGALGSVAKAVVAMRSLTLLRHLRSDTRAYRAAAQAEHLDGA
                     QLAALPGAALDVRVRLLRDRIHQGWILTAIWLVDTGVTAAALEHTRAASSVAGFDMIM
                     ESGLISAETAELAAALRGDPPLRALAREGNVSSILALSSNTAGALESAVTRIGHRGPG
                     EAELASTVFSDDPAKLLMAASDDAPTEPTPPSTLSRRLAANARGSRELAHDTTIRFTH
                     ELRMTLRELGSRRVAADQIDVVDDVYYLTCDELVTMPADARLRIKRRRAERERLQVQR
                     PPDVIDGTWSPVDRSAASD"
     gene            complement(7888..>8806)
                     /locus_tag="VFI55_01345"
     CDS             complement(7888..>8806)
                     /locus_tag="VFI55_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7072713.1"
                     /translation="ATGSASRRPPIPGSDADGVYYLRTYDDAAALNSVLTEGSSLAVV
                     GAGWIGLEVAAGARQRGVGVTVVEAAKQPLAAALGEKVGEVFATLHRDHGVDLRLETQ
                     VEEITRSDGPEGKATGLKMRDGSTVAADAVLVAVGAKPNIELAEEAGLSMGDGGVLVD
                     ASLRTSDPDIYAVGDIAAAENPLFGTRIRTEHWANALKQPAVAAAGMLGTPGEYTELP
                     YFFTDQYDLGMEYVGHAPSFERVVFRGDVPGREFVAFWLDGGNRVLAGMNVNIWDVLD
                     DVKSLIRSRTPVDVDKLTDPKAPLADLLS"
BASE COUNT         1382 a         3069 c         2990 g         1365 t
ORIGIN      
        1 acgccatgat catcgcgatc ccccaggcgc ctccgatcgt cgatgcatcg aatcccgctg
       61 cccggcttag cttttgaccg cggcgcgaac gccgcgggcg ttgatcgcag cggcaatgcc
      121 ccgcaaatta ggtacctgac cggcaggtac ggtgtcctga tggtcgacgg gatgctcgct
      181 gccgtgcgcg tcctcgacct gtccagcggt aacgcggacc ccgtgacccg gctgttcgcc
      241 gacctgggtg ccgacgtcct caaggtggaa cccccgggcg gaaacctggg gcgcgacgaa
      301 ttgccgacac tggccggcgc gagcattccg ttcgcggtgc ataacgccaa caaacgcagc
      361 gcggtgctgg acccgttcga cgaaaacgac tgccgcctct tcctcgacct cgccgcgtat
      421 gccgacatcg tcgtggacag cggcattgac ggactggccg acgcgttcgg ggcgccgggt
      481 gtggacttgg ccacccgcta cccgcacctg gtggtgctat cgatcaccga cttcggcgcg
      541 acgggtccgc gctcatcgtg gcgtgccacc gatcccgtgc tgtacgccat ggggggctca
      601 ctgtcgcgat ccgggcccac caccggtacc cccgtgctgc cgccggacgg tattgcttcg
      661 gcaacggccg ccgtgcaggc ggcctgggcc gcgctggccg cctactacaa ccgattacgt
      721 tgcggcaccg gcgattacat cgacttctcc cggttcgatg ccgtcgtgat ggcactcgac
      781 ccggcgttcg gcgcgcacgg gcaggccgcc gccggaatcc gcggcacccc gcagtggcga
      841 gggcggccaa agaaccagga cgcgtacccg atctatccgt gccgggacgg ctacgtccgg
      901 ttatgcgtgc tggcgccgcg acagtggcgc ggcctgcggc gctggctggg agagccgcaa
      961 gaatttcagg acccgaaata cgacgccatc ggcgcccggt tcgcggcctg gcccgagatc
     1021 agcgtgctga tccgggcact gtccgccgaa cacaccatga aggaactggt ggccgccgga
     1081 caggcgcacg gggtgccgat cgccgcggtg ctggcgccgt cacagatcct gggctccgag
     1141 cattttcagg cggtgggcgc gatcaccgac gccgagctgg tccccggggt ccgcaccagc
     1201 gtgcctaccg gatatttcgt ggtcgacggg cagcgggcgg gttttcgcac accggccccc
     1261 accgcgggac aggatcagcc gtactggctc gccaacccac cggcggtgcc atcgccggcg
     1321 ggcaggcagg gcggttatcc gtttgcggga ctgcggattc tcgatttggg cgtcatcgtc
     1381 gccggcggtg agctgagccg actgttcggc gatctgggcg ccgaggtcat caagatcgaa
     1441 agcgccgact acccggacgg attgcggcaa gcccgcgccg gcgacgccat gagcgagtca
     1501 ttcgcgtgga cacaccgcaa ccaactcggg ctcggcctgg acctgcgcag caccgagggc
     1561 gcccgtatct tcggcaggct ggtcgcgcag gcggatgcgg tcttcgctaa cttcaagccg
     1621 ggaacccttg cttcacttgg gttttcctac gacgcgctgc gcgcgatcaa cccgcggatc
     1681 gtgctcgcgg agagcagcgc attcggcgac cggggcccgt ggagcgcccg gttgggctac
     1741 gggccgctgg tccgcgccgc caccggcgtc acccggcttt ggacatccga cgatccggac
     1801 ggccggtccg acaccgcgcg gcacgccttt tacgacgcga cgacgatctt tccggaccac
     1861 gtggtcgggc gggtcaccgc attcgcggcg ctggccgcgc tgatccaccg cgacaggacc
     1921 ggcagaggag cccacgttca tatctcgcag gccgaagccg tcgtcaatca gctcgacacc
     1981 ctgtacgtca cccaggccgc gctcgaggcg ggcatctccc ggatccgcag tgacacgagc
     2041 ctgcacgccg tctgcccgtg cgcgggcgac gacgaatggt gcgtcatctc gattcagtcc
     2101 gacaacgaat ggcgtagtgc cgcatcggtt ttcgaccatc cagcatgggc cgacgatccg
     2161 cgtttcagct cggctgagtc acgcctggcg aaccggcacg agctggtggg gctggtgtcg
     2221 gcctggacac ggatgcgcac cccggtgcgg gccgccgaac tgctgcaggc ggcgggtatc
     2281 gcggccggcc cgatgaaccg tccgccggac cttctggaag accctcagct ggcagggcga
     2341 aaggtgtact gcgacatggt gcatcccctg tttgaccatc cgctgccggc ggagacgggt
     2401 ccggcgccgt ttcagcacat cccgccggcc ccgcaacacc ccgcacccct cccgggtcag
     2461 gacacccgcg aggtttgccg aaacctgctc gggatgaccg ccgacgagac cgagcggctg
     2521 attcacgatc gcgtcctgtt cgaaccgacg ggcggcgcct agcatttttg tcacacctct
     2581 cggttcccga gcacaggaca acccatgaca gctacctcag tgccaaccga gctcagcgca
     2641 cggtatttca tcggtggccg gttcttcacc gcggacaacg tccagccggt ggtggaggcg
     2701 gccaccggcg aaccgctcgg cgacggcgcc tgcgtcaccg aatccgatat cgacgacgcg
     2761 gtggccgcgg cgcgtcaggc ccttcccgag tggcggtcga gctcgccgga tcaccgcgcc
     2821 gggctcctca acgcgttggc agccgcactg aagtcacgcg ccaaggccac cagtcagctg
     2881 gtgagccggg agaacggcat gccgatctcg ctgtcccgcg gcgccaacgg cgccttcccg
     2941 ccgttgctgt tgcggtacta cgcacagctg gtcacggaga tcccgatcga agagatccgg
     3001 cccggtgtga ccggccacac gatcgtgcgc cgcgaggccg tcggggtggt tgccgcgatc
     3061 atcccgtgga actatccgca ggcgctggcc gtcatgaaga tcgcaccggc gctagccgcg
     3121 gggtgcacgg tggtactcaa ggcctcgccc gaaaccgcct tggatgcatt ggttttcgcc
     3181 gacgccgccg tcgaagccgg gctgccgccg ggggtattga acgtggtgtc cggcgggccg
     3241 gcgaccggtg cttacctggt ggcgcacccg ggtgtggaca aagtcgcgtt caccggttcg
     3301 accgccaccg ggcgcgtcgt ggcggaaatg tgcgggcggc tgatgcgtcc cgtcaccctc
     3361 gagctgggcg gcaagtccgc ggcaatcatc ctcgacgacg ccgatctgga cgtcacgatg
     3421 cgcggattgc gcacggcgtc gttcgtcaac aacgggcaga cctgccacct gagctcccgc
     3481 atcctggcgc cgcggtcccg gtacgacgag atcgtcgatg cggtcgcggc actggccgaa
     3541 agcatgacgg tcggcgaccc gctgaacccg tccactgaga tcggcccgct ggtcagctcg
     3601 cgccagcgcg accgggtgct cagctacatc gcggtcggaa aggacggcga cgcgaggttg
     3661 gtcgccggcg gatcggtgcc gaaggaccag ccgcggggct ggttcgtgtc gccgaccgtg
     3721 ttcgccgacg tcgacaactc ggatcgtctg gcgcgcgagg agatcttcgg cccggtgctg
     3781 accatcagcg cctacgacac cgacgaccag gcgatcgcgc ttgccaacga cagcgaattc
     3841 ggtctcgccg gcaccgtgtg gtcggccgac accgaacgcg ccaccaccgt cgcccgcgcc
     3901 gtgcacaccg gctcgatcgg ggtcaacgac tacgcattgg acgtgcgtgc gccgttcggt
     3961 ggggtcaagg ccagcgggtt gggtcgcgag ctgggccccg aggggctcga ggcctaccgg
     4021 acactgaagt cgatctaccg cacgggctcg gcgtagcagc gtcgccctcg agcgccgaac
     4081 gtgcggctgg ccgcacgctc gcaaccgaac gtgcggctgg ccgcacgttc gatgcccacg
     4141 gccgggcgcg agcggtcgtc agataggttc gctatatgac cgtcgatccc aggacaccgg
     4201 tgttgatcgg ctatggccag gtcaaccacc gcgacgaaat cgaccccgca acgcggtccg
     4261 tcgaaccggt cgacctgatg gccgccgcgg cccgccgagc tgcggattcc cgggtgatcg
     4321 aggcagtgga ttccatccgt gtggtgaacg tgttgtcggc gcactaccgc gatcccgggc
     4381 gcctgctcgg cgaccggatc ggagccaccg gcttcaccac gctgtacagc ccggtcggcg
     4441 gcaacgtgcc gcaatcgctg gtcaaccagg cctgcctgga catccagcgt ggtcgcgcaa
     4501 atgtgattct gctcgccggc gccgaaacct ggcgcaccag gaccgggttg aaggccaaag
     4561 gcagcaaact cacgtggacg gtccaggacg agtccgtgcc gatggccgag gtcagcggca
     4621 ccgacgtccc aatggccggt gcggcggagc tcaggatcca cctcaaccgg ccggcccacg
     4681 tctacccgct cttcgaacag gcgcttcgga tcgccaacgg cgagtccgtc aaggaccatc
     4741 tcaaccgcgt ggccgagctg tgggcacgct tcaacgccgt cgcggtcagc aacccgcacg
     4801 cgtggattcg caaaccggtg accgccgagg aaatccggcg acccggcccc cagaatcgga
     4861 tgatcagctg gccctacacc aagctgatga actccaacaa catggtcgac cagggcgccg
     4921 cgctggtcct gacgtcggtc gagcgggcga gaggcctgcg gatccccgcc gagcgctggg
     4981 tttacccaca cgcgggcacc gacgcgcacg acaccccggc cgtcgccgac cgtgacgaac
     5041 tgcaccgttc gacggccatc cggatcgccg gcaggcgggc gctcgaactg gccggcctgc
     5101 acatcgacga tgtggactac gtcgacctgt actcctgctt tccctccgcc gtccaagtcg
     5161 cggcggccga actgggcctg agcgtcgagg attccgcccg gccgctcacc gtcaccggcg
     5221 gcctgacgtt cgcgggcggc ccgtggagca actacgtgat gcattccatt gccaccatgg
     5281 ccgaattgct ggtcgccaac cccgggcgcc gggctctgat caccgcaaac ggcggctacc
     5341 tgaccaaaca cagcttcggg gtgtacagca ccgagccgcc cgcggagttc cgatgggagg
     5401 acatgcaacc ggcggtcgac cgagagcaca cccgggaagg cttggtcgag tgggagggcg
     5461 tcggcaccgt cgaagcctgg acgacaccgt tcaaccgcga gggacaaccg gagcgggcgt
     5521 ttctggccgt gcgcacgccc gatgggtcgc gcgcactggc ggtcatcgcc gatcccactg
     5581 ccgctgaaac caccgtgcgt gaagatatcg gaggcgccaa ggttgccgtc gccgcggacg
     5641 gcagcgccgc gctgctgtaa ccggccaagg gaatcattac tggccagggc acgtttggca
     5701 gcgtaacggt cgcgcgagtc aggccgacgt gcctattgtc aaggtggacg ttgggggagg
     5761 tgagcccagg tgcagatcct ggttaccgac gccaccggcg cagtcgggcg gctggtcgct
     5821 cagcagctca tcgctgctgg gcacacagtc agcggcattg ctgagttccc gcacccctac
     5881 ctggacccag atgtcgagtt tgtttgcgcg tcgctgacag atcccgccct gcaggacctg
     5941 tccgacgaag ccgacgtggt gattcacctg gccccagtcg acaccaccgc acccggcggc
     6001 gtgggcatcg acggggtggc gcacgtcacc cacgcggccg cccgagccgg tgcgcggctg
     6061 ctgttcgtgt cccaagccgc gggccggccc gatctgtatc ggccggccga ggagctggtg
     6121 tcgacgagtt gggcgcccag tttggtcgtc aggatcgcgc cgccggtcgg acgccagctc
     6181 gactggatgg tgtgccgcac cgtggccacg ctgctgcgca gcaaggtctc cgcgcagccg
     6241 atgcgcgtgc tgcacatcga cgacctggtg cgcttcctgg tcatggcgct gaacaccgat
     6301 cgcaccggtg tggtggacct ggccaccccg gacaccacca atgtgatcac cgcttggcgg
     6361 atgctgcgat cggccagccc gcgttcccgg ctgcgcgggg tgcgcagctg ggcacagttg
     6421 attccggaga tggatattgc cgcagcacaa gaggattggg cgttcgaatt cgggtggcag
     6481 gcgttagagg cggtcgccga caccgcgcgc ggactggtcg ggcgccgctt ggaggacacg
     6541 ggtgcgacca gccgcggggg ccaactggca ctcccggtgg aggttgcccc gcgccccctc
     6601 tcatccgacg agctgcaccg cgccgcccct gagggtatgg aggccgagtt cgacgaccgg
     6661 atcgatgctc ggttcccggt cttcagcgcg gcccgcctcg ccgaggcact gcccggaccg
     6721 ctcaccccga taaccctgga cgtccagttg agcgggctgc gtgcggccag ccgggtgatg
     6781 ggccaggtgc tcgcactcgg cggcgtggtg gacgacgagt gggggagcag ggccatcgcg
     6841 gtgttcggtc atcgcgccta catcggggtg tcggcgaacg tcgtggccgc ctctcagctg
     6901 cccggctggg accaggacgc cgtcgcccgg cacgcgttgg tcgaccagcc ccaggtgggg
     6961 gacgtcctgc cgttcggcga gcctcggctg gccgagggcg cgctggggtc ggtcgccaaa
     7021 gcggtcgtgg cgatgcggtc gctgaccctg ctccgccatc tgcggtccga cacgcgggcc
     7081 taccgtgccg ccgcccaggc ggaacacctc gacggcgcac agctggccgc gcttccgggc
     7141 gccgccctgg acgttcgggt tcggctgctg cgggatcgca tccaccaagg ctggatcctc
     7201 acggcgatct ggttggtcga cacgggcgtc accgcggcgg cgctggagca cacccgggcg
     7261 gcgtccagcg tggcgggctt cgacatgatc atggaatccg gcctcatctc cgccgagacg
     7321 gccgagctgg ctgcggcgtt acgcggcgac ccgccgttgc gcgcgctggc ccgggaaggc
     7381 aacgtcagca gcatcctggc gttgtcctcg aacaccgccg gcgctctcga gtccgctgtc
     7441 acccggatcg ggcaccgtgg cccgggggaa gcggagctgg ccagcacggt gttcagcgac
     7501 gacccggcga agctgctgat ggcagcatct gacgacgcgc ccaccgagcc gacgccaccg
     7561 tcgacgctgt cccggcggtt ggccgccaac gcgcgcggtt cgcgggagct ggcccacgac
     7621 accaccatcc gattcaccca cgagctgcga atgacgctgc gagagctggg atctcggcgc
     7681 gtcgcggccg accagatcga cgtcgtcgac gacgtgtatt acctgacctg tgacgaactg
     7741 gtcaccatgc cggccgacgc ccggctgcgg atcaagcgtc gccgcgccga acgggaacgc
     7801 ttgcaggtgc aacgtccacc cgacgtcatc gacgggacgt ggagcccggt cgaccgaagc
     7861 gccgcgtctg actgacgccc cgcgccgtca gctcagcagg tcggccagtg gcgccttcgg
     7921 atcggtcagc ttgtcgacgt cgacgggagt tctggaccgg atcagggatt tgacgtcgtc
     7981 gagcacatcc cagatgttga cgttcatccc ggccagcacc cgattgccgc catcgagcca
     8041 aaacgcgacg aattcacggc cggggacgtc accgcggaac accacccgct cgaagctggg
     8101 agcgtgccca acgtactcca tcccgaggtc gtactggtcg gtgaagaaat agggcagctc
     8161 ggtgtattcg cccggcgtgc ccagcattcc ggccgccgct accgcgggct gcttgagggc
     8221 gttggcccag tgctcggtgc ggatccgggt gccgaacagc gggttctcgg cggcggcgat
     8281 gtcaccgacc gcataaatgt cgggatcgct ggtgcgcagc gaagcatcca ccagcacccc
     8341 gccgtcaccc atggatagcc cggcctcttc ggcgagttcg atgttcggct tggcgccaac
     8401 ggcgaccagt acggcatcgg ccgcaaccgt cgagccgtcg cgcatcttca gcccggtggc
     8461 tttgccctcc ggcccgtcgg accgggtgat ctcctcgacc tgcgtttcta gcctcagatc
     8521 tacgccgtga tcgcggtgca atgtggcgaa cacttcgccg accttctctc cgagcgcggc
     8581 cgccagtggt tgtttggccg cctcgacgac ggtgacgccg actccgcgct gacgggcacc
     8641 ggcagccacc tccaggccga tccagccggc gcccaccacc gcaagcgaag acccttcggt
     8701 cagaacggaa ttcagcgccg cggcgtcgtc gtaggtacgc aggtagtaca ccccgtcggc
     8761 atccgagccg ggtatcggtg gccgccgcga cgcggatccc gtcgcc
//
LOCUS       DASSGQ010000032        24443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_913520,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000032 DASSGQ010000000
VERSION     DASSGQ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 24443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24443
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913520"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1177)
                     /gene="carA"
                     /locus_tag="VFI55_01350"
     CDS             complement(47..1177)
                     /gene="carA"
                     /locus_tag="VFI55_01350"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET7072714.1"
                     /translation="MNKALLVLEDSRVFTGKPFGKVGQTLGEAVFSTGMSGYQETLTD
                     PSYHRQIVVATAPQIGNTGWNGQDGESRGDKIWVAGYAVRDPSPRASNWRATGTLDEE
                     LVRQHIVGIAGIDTRAVVRHLRSRGSMKAGIFSGASLADPAELVERVRGQQSMLGADL
                     AAEVSTAETYVVEPEGPAGFPRFTVAALDLGIKTNTPRNFARRGIRSHVLPSSVTFEQ
                     IADIKPDGVFLSNGPGDPATADHVVALTREVLGAGIPLFGICFGNQILGRALGLSTYK
                     MVFGHRGINVPVIDHATRRVAVTAQNHGFALEGEAGQRFDTPFGAAIVSHTCANDGVV
                     EGVKLVDGHAFSVQYHPEAAAGPHDAEYLFDQFVDLMAGEGR"
     gene            complement(1227..1748)
                     /locus_tag="VFI55_01355"
     CDS             complement(1227..1748)
                     /locus_tag="VFI55_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET7072715.1"
                     /translation="MNSGTLVGSLIFAAVLVVVIAVLIQLMMRGWHRRGQRQAELIGD
                     LPDVPDAVGSATTTIRGLYVGSTLSPAWNERITAGDLGYRSKAVLTRFPEGILLERIR
                     AKPIWIPQEEITAVRTERGVAGKVAARNGILAIRWRLPSGVEIDTGFRADNRGEYGGW
                     LEGWPDGRQEGAE"
     gene            complement(1798..3102)
                     /locus_tag="VFI55_01360"
     CDS             complement(1798..3102)
                     /locus_tag="VFI55_01360"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET7072716.1"
                     /translation="MSVLLRGVRLYGEGDRVDVLVSAPGREGQIAEIGPGLAIPDHAD
                     VIDAPGHVLLPGFVDLHTHLREPGREYAEDIETGSAAAALGGYTAVFAMANTNPVADS
                     PVVTDHVWHRGQQVGLVDVHPVGAVTVGLAGAVLTEMGMMAAGAAQVRMFSDDGICVH
                     DPLMMRRALEYATGLGALIAQHAEEPRLTVGAVAHEGPTAARLGLAGWPRAAEESIVA
                     RDALLARDAGARVHICHASTAGTVEILKWAKAQGISITAEVTPHHLLLDDSRLANYDG
                     VNRVNPPLREAADAIALRHALAEGVIDCVATDHAPHAEHEKCVEFSAARPGMLGLQTA
                     LSVVVQTMVAPGLLTWRDVARVMSENPARIVGLPDHGRPLEVGEPANLTVVDPEATWT
                     VAGSDLASRSANTPYESMTLPATVTATLLRGKVTARDGKISA"
     gene            complement(3099..4073)
                     /locus_tag="VFI55_01365"
     CDS             complement(3099..4073)
                     /locus_tag="VFI55_01365"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET7072717.1"
                     /translation="MTRHLLAAADLSREVATAILDDADGFAQALVGREIKKLPTLRGR
                     TIVTMFYENSTRTRVSFEVAGKWMSADVINVSAAGSSVGKGESLRDTALTLRAAGADA
                     LIIRHPASGAAHLLADWTSSFAVGDSRGLGPSVINAGDGTHEHPTQALLDALTIRQRL
                     GGIEGRRIVIVGDILHSRVARSNVMLLDTLGAEVVLVAPPTLLPVGVEEWPVTVTHDF
                     DAELPAADAVLMLRVQAERMNGGFFPSVREYSVRYGLSGRRQAMLPGHAVVLHPGPMV
                     RGMEIASSVADSSQSAVLQQVSNGVHVRMAVLFHVLVGTESAQNEGAA"
     gene            complement(4070..4654)
                     /gene="pyrR"
                     /locus_tag="VFI55_01370"
     CDS             complement(4070..4654)
                     /gene="pyrR"
                     /locus_tag="VFI55_01370"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET7072718.1"
                     /translation="MGAAGDTGLGRGSRELMSAADVGRTISRIAHQIIEKTALDGPDA
                     PRVVLLGIPTRGVILANRLTVNISEYSGVDVGHGALDITLYRDDLMKKPPRPLAATSI
                     PAGGVEDALVILVDDVLYSGRSVRSALDALRDVGRPRAVQLAVLVDRGHRELPLRADY
                     VGKNVPTSRSESVHVQLREHDGCDGVVITRDGDS"
     gene            4789..6078
                     /locus_tag="VFI55_01375"
     CDS             4789..6078
                     /locus_tag="VFI55_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HET7072719.1"
                     /translation="MFDLRELAAVDPHADEAGLIARITELERAKSAAAAGQARAAAAL
                     DALRRSNEADADVPAAHRGRGVSSEIALARRDSPARGGRHLGFAKALVYEMPRTLAAL
                     EAGLLSEWRATLIVRESACLDVDDRHELDAELCADESKLDGMGDARIAATARAIAYRL
                     NAQAVVDRAAKAASERTVTIRPAPDTMTYVTALLPVAQGVSVYAALKRAADTTFDDRT
                     RGQVMADTLVERVTGRPADVAQPVAVNLIIADETLLGGDDIAAVVDGYGPIPAAVARN
                     LVSNATTDKRSQATLRRLYRHPRSGALLAMESRARRFPKGLATFIGLRDQRCRTPYCD
                     APVRHRDHAQPRHRGGATNAANGLGLCERCNYVKEAPGWRVTAAMDETGRHMAEFTTP
                     TGMRYQSGAPPAPGPLEIFVSQVETRIGVALAKLHAA"
     gene            complement(6109..6537)
                     /locus_tag="VFI55_01380"
     CDS             complement(6109..6537)
                     /locus_tag="VFI55_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose-binding domain-containing protein"
                     /protein_id="HET7072720.1"
                     /translation="MTGLNNYVKRWRTALHVTVSALIVAILGLAITPVAHAAAAAATL
                     SVEHTWQTGFIAHFTIINSSMAPLTDWKLEFDMPVGQGVSHTWNSTLTQSGTHYVLTP
                     ANWNRTIAPGGSATGGLRGVLTGSYSPPSNCLLNGQYLCT"
     gene            6718..6987
                     /locus_tag="VFI55_01385"
                     /pseudo
     CDS             6718..6987
                     /locus_tag="VFI55_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            7171..7530
                     /locus_tag="VFI55_01390"
     CDS             7171..7530
                     /locus_tag="VFI55_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072721.1"
                     /translation="MTVVRTELARQFYFGDDAVLLVMDTGGVATFLSPLLSAHQQGAS
                     RLEHGGTVHDFLIEPGAADIELHGDRVVWRLDHAKAAEIIELLTAMTQSSTGAGHYYV
                     DISTPAETLVLSRNEHV"
     gene            complement(7680..8804)
                     /locus_tag="VFI55_01395"
     CDS             complement(7680..8804)
                     /locus_tag="VFI55_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072722.1"
                     /translation="MAATATLPTLSQVQTLDTAYLRGAAEYWTRTANLWEQAFADVHD
                     TMSIPGGSPWKGRAAAAAQERSHLDLVKVREASDQLHGAAGLALRGDQQLQACKDGVL
                     EAVNDARVDGFHVGEDYSVTDRLRGGTPEFRAARLAAAQGHASFIRHRLAALLTSDQQ
                     LTTRITAATKDIDVLNFHEGSGVQAVDRHTFKDAPNPEPDPPPPPARGLPPDGVHPPV
                     EGNLTVGPASRPSEEAKGGLSLWDDKGGEWRYDPGANQWHNPHWDYNPHDTKFSQWQN
                     IPMGELPPHTAKAAPKAPVPGPAPSPASKPQPAPRPAPPPVEAPPARPVPPESGGMPI
                     IPGGPATPLGPHVIHPPHSIPHHFPILGEDDPAEGARDFE"
     gene            complement(8805..9116)
                     /locus_tag="VFI55_01400"
     CDS             complement(8805..9116)
                     /locus_tag="VFI55_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072723.1"
                     /translation="MESGRTAGELQVSEGGLRAQANWCEALATRLATNSAPAGAGSSP
                     LASSAAVNISHAQVVAAGMRCATRMQTTAGKLAAASAGYGSNEASSAALMRALRPVTV
                     F"
     gene            9205..10410
                     /locus_tag="VFI55_01405"
     CDS             9205..10410
                     /locus_tag="VFI55_01405"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7072724.1"
                     /translation="MNLDGNQAAIREVCEAGLLAGAVTVVWQRGEVLQLNEIGYRDID
                     AGLPMTRDTLFRIASMSKPVTVAAVMSLVDQGKLALKDPILRWAPELADVQVLDDPHG
                     PLDRTHPARRAILIEDLLTHTSGLAYAFSVSGPISKAYMRLPFNQGPDVWLSELAKLP
                     LVHQPGERVTYSHSIELLGIIAARVEDKPFHQVLDERVLDPTGMPDTGFFVSVEARRR
                     AATMYSLTEEHVLQHGVMGPPHITPPSFCNAGGGLWSTADDYLRFVRMLLGDGSVDGV
                     RVLSPESARLMKTDRLTDEQKRHNFLGAPYWVGRGFGLNLSVVTDPAKSAPLFGPGGL
                     GTFSWPGAYGTWWQADPTADLVLLYLIQNLPDLTVDAATAVAGNTSLAKLRTAQPKFV
                     RHSYRAVGL"
     gene            10415..10954
                     /locus_tag="VFI55_01410"
     CDS             10415..10954
                     /locus_tag="VFI55_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7072725.1"
                     /translation="MVDYPPVRIIGPRLVLRPPVLDDAGALFQRVARDPEVTRYLLWA
                     PHSDVGVTRRVIAEKMNVSDDERTWVIELKHRGEVIGLTSCRRKLPHSVEIGYCLGRR
                     WWGKGLMAEALDMLLDALAGDRLVHRVWATCSPYNERSARLLEGAGFVLEGRLAHHAV
                     YPTMGPEPQDSLLYAKALR"
     gene            complement(10989..13691)
                     /locus_tag="VFI55_01415"
     CDS             complement(10989..13691)
                     /locus_tag="VFI55_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HET7072726.1"
                     /translation="MMQMPDLAPLRLDCDVLVIGGGTAGTMAALSAAERGAQVLLLEK
                     AHVRHSGALAMGMDGVNNAVIPGKAEPEDYVAEITRANDGIVNQRTVYQTATRGFAMV
                     QRLERYGVKFEKDEHGEYAVRRVHRSGSYVLPMPEGKDVKKALYRVLRQRSMREKIRI
                     ENRLMPVRVLTDASQDGRAVGAAAFNTRTGEFVAVGAKAVILSTGACGRLGLPASGYL
                     YGTYENPTNAGDGYAMAYHAGAELSGIECFQVNPLIKDYNGPACAYVANPFGGYQVNS
                     HGERFVDSDYWSGQMMAEVKSEIDSARGPIYLKVSHLSDETLTALENILHTTERPTRG
                     TFHANRGHDYRTHDVEMHISEIGLCSGHSASGVWVDEHARTTVPGLYAAGDLACVPHN
                     YMIGAFVFGDLAGTDAASTCEEVAAPQTLPEDQLRAAHELIYRPLRHPDGPPQPQVEY
                     KLRRFVNDYVAPPKTAAKLSIAVRTFDRMQAEIADMGARNPHELMRAVEVWFIRDCAE
                     MAARSSLTRTESRWGLYHDRADLPVRDDSQWGYHLNLRKGTDGEMVFLKRPVAPYFVP
                     VPELDGLPPSDQTVQAVEQPPLVGGQAPATTRSRVASAATTFEPPSPRIAKVLALEEP
                     TIADLGPYLADSDPGVRRTAVATLTEHIPDGYAPALFAVLDDADAAVRGTAAEGIREL
                     VEVLPDPADARAYLGSRDTVVRAAAVYLLYARRAGDAGEYRGALTDADHRVRIEAVRA
                     LVSVDDVAGVVEATRDESREVRIAAAAGLATLRDGAQAVSALIADPDPLVRAAALAAL
                     GELGCRQDDFAAIERALRAPAWQVREGAARALSGASEELAAPAVSGLFAALGDPHLDV
                     RKAAVLSLTRWAGEPAARDALGTALKDSDADVRAYARRALEQGG"
     gene            complement(13688..14431)
                     /locus_tag="VFI55_01420"
     CDS             complement(13688..14431)
                     /locus_tag="VFI55_01420"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7072727.1"
                     /translation="MTVGVSLELDRVRLSYTGVPVIEGLSLVARPGEILVLTGPSGCG
                     KSTVLRALAGLLSPDDGRVLADGAEVTTTSGDRGMVFQDNALLPWRSVRSNIELALRL
                     RGVPRAGRRTEADRWIAELGLTGFGDYLPKSLSGGMRQRVQLARGLAGAPRAVMMDEP
                     FGALDTQTRSAMQRLLIDTWRAHPTTIVFVTHDVDEALLLGDRIAVLGNAGRPLRALI
                     DVAEPRSDRQRPALRAEIIAALDHSAVAA"
     gene            complement(14428..15273)
                     /locus_tag="VFI55_01425"
     CDS             complement(14428..15273)
                     /locus_tag="VFI55_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7072728.1"
                     /translation="MTAQLIDQTVSAPILGTPRQAASPWRSRLLRLASVAAAIGLWQV
                     LTAGKVRFWLRFDTLPTVTQIAHALTRRVQTYEYWLDLAQSLLRILTGFGIAAVVGVA
                     TGVLLGRSRLFADVVGPLTELARPIPAIAMVPVAILLFPTDEAGIVFITFLAAFFPIM
                     VSTRHAVRALPTLWEDAVRTLGGGRWEVLAQVVLPGILPGVFGGLSVGMGVAWICVIS
                     AEMISGRLGVGYRTWQDYTVLSYPQVFVGIITIGVLGFVTAAAVEVVGRRVTRWLPRA
                     EEEQR"
     gene            complement(15270..16658)
                     /locus_tag="VFI55_01430"
     CDS             complement(15270..16658)
                     /locus_tag="VFI55_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7072729.1"
                     /translation="MKRHIARLLSVVIAVASLTSGCSLESLSESSGVVNVVIGYQSKT
                     INTVTAGTLLRAQGYLERRLADITTRTGTKYSVRWQDYDTGAPITAQMLAEKIDIGSM
                     GDYPMLINGSKTQANPLAHTEMISVTGYNPKGALNMVVVEPDSTATGLSDLAGAKVSA
                     SVGSAGHGTLVRALDRAGIKGVQVLNQQPQVGASALESGQVQGLSQFVAWPGLLVYQG
                     KAKLLYDGAELNLPTLHGVVVRRTYAASHPEVLAAFLQAQLDATDFLNQKPLQAARIV
                     AQASGLPQEVVYLYNGPGGTSFDTTLKPSLVDALKNDVPYLKSIGDFADLDVSGFVQD
                     GPLRTVFGARGLDYDAARAATANPSLLGGDPALASELWLDGSDTTQTLANPTSLLRAV
                     RDAIAHGAKVRAAYVPDAELGTRWFADKAFWVQDGQSYRPFGTLAGARRYLAAHPGGV
                     AVNYQQALGGSL"
     gene            complement(16684..16911)
                     /locus_tag="VFI55_01435"
     CDS             complement(16684..16911)
                     /locus_tag="VFI55_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019931803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HET7072730.1"
                     /translation="MTLVNNRADVPVTIDESLCIDGCTLCVEVCPLDALAINPDTGKA
                     FMHVDECWYCGPCAARCPTGAVTVNMPYLIR"
     gene            complement(16908..17660)
                     /locus_tag="VFI55_01440"
     CDS             complement(16908..17660)
                     /locus_tag="VFI55_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7072731.1"
                     /translation="MAQPVPIAGPRADRARRVADVLRQQIHAGAYPDGLPAEQELAAE
                     FFVSRNAVREALAVLKREGLIERGPKVGTHVAQRKYEHGLDALLGLKETFKNLGEVRN
                     EVRAAMLVTAPPSVARRLRLEPGEQAVFVERLRYLGDLPLSLDLTYLAPDIGTQILDY
                     PLETNDLFALIEQVSGQRLGSASLALEAIPADAHSAATLEVPDGAALLMLERLTSLDD
                     GRPVDLEYIRMRGDRITMRGSLIRAEPIRSKP"
     gene            complement(18061..20916)
                     /locus_tag="VFI55_01445"
     CDS             complement(18061..20916)
                     /locus_tag="VFI55_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7072732.1"
                     /translation="MNRNSARPRRLRVSALAAVANPSYTRLDTWNLLDDACRHLAEVD
                     LAGLDKGHDVARVKRLMDRIGAYERYWLYPGADNLAIFRAHLESLSTVRLTEEVSLAV
                     RLLSEYGDRAALFDTSAPLDDQELVAQAKQQQFYTVLVADDVPPTAPDCLAESLRALR
                     NPAEDVQFEVLVAPSVEDAITAVALNGEIQAAIIRHDLPLRSRDRVPLMNTLLGPNDS
                     EAVSPDCAQDWVECGEWIKELRPHIDLYLLTDESIAAGNDDEPDVYDRTFYRLNDVTD
                     LHSTVISGLRNRFATPFFDALRAYASAPVGQFHALPVARGASIFNSKSLQDMGEFYGR
                     NIFMAETSTTSGGLDSLLDPHGNIKKAMDKAAITWNSNHTYFVTNGTSTANKIVVQSL
                     TRPGDIVLIDRNCHKSHHYGLVLAGAYPLYLDAYPLPQFAIYGAVPLRTVKKALLDLE
                     AAGQLHRVRMLLLTNCIFDGVVYNPRRVMEEVLAIKPDICFLWDEAWYAFATAVPWAR
                     QRTAMVSAERLEQTLASPEYAEEYRNWAASMQGIPRSEWADHRLLPDPGRARVRVYAT
                     HSTHKSLSAFRQASMVHVRDQDFNSRARDAFGEAFLTHTSTSPNQQLLASLDLARRQV
                     DLEGFQLVRQVYDMALVFRHRVRKDRLISKWFRILDESDLVPEEFRASAVSSYREVRQ
                     GALAEWNEAWRSDQFVLDPTRVTLFLGNTGMNGYDFREKILMDRFGIQINKTSINSVL
                     LIFTIGVTWSSVHHLLDVLRRVAADFDREKQGASAADWALHQRRVEEITQDLPHLPDF
                     SEFDSAFRPVDACSVGDMRAAFYAGYEETDREYVLIGAAGRRLADGKRLVSTTFVVPY
                     PPGFPVLVPGQVVSKEIVYFLAQLDVKEIHGYNHDLGLSVFTQAALARMEAQRNAATA
                     AVGAAVPIFELSSDAASLGALGAVNGDRSVRTAANDG"
     gene            complement(21055..21453)
                     /locus_tag="VFI55_01450"
     CDS             complement(21055..21453)
                     /locus_tag="VFI55_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein NusB"
                     /protein_id="HET7072733.1"
                     /translation="MTRLELRVVLAAALAATVVLGAVACAAFGLAIVASTLSIYALAV
                     GAWLYHSIERLVLARRIRTVRTAAKPLAPLLPLMAAIMGLTQAVVRSLSDVTELPMRP
                     WDLPVLRWVDATRFSRRSVDGECGDDELEC"
     gene            complement(21450..21968)
                     /gene="nusB"
                     /locus_tag="VFI55_01455"
     CDS             complement(21450..21968)
                     /gene="nusB"
                     /locus_tag="VFI55_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET7072734.1"
                     /translation="MPERKTGPDPKPARGKGRHQARKRAVDLLFEAEARGLSPAEVVD
                     VRGALAGTNPDVAPLNAYTAAVARGVSEHSAHIDDLISSHLVGWTLDRLPAVDRAVLR
                     VAVWELLYADDVPEPVAVDEAVQLAKELSTDESPGFVNGVLGQVLLVTPQIRAAAAAV
                     RSGAQDDRGPQP"
     gene            complement(21971..22534)
                     /gene="efp"
                     /locus_tag="VFI55_01460"
     CDS             complement(21971..22534)
                     /gene="efp"
                     /locus_tag="VFI55_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET7072735.1"
                     /translation="MASTADFKNGLVLVIDGQLWTITEFQHVKPGKGPAFVRTKLKNV
                     LSGKVVDKTYNAGVKVDTATVDRRDTTYLYRDGSDFVFMDSQDYEQHPLPETLVGDAA
                     RFLLEGLPVQVAFHNGAALYIELPVTVELEVTHTEPGLQGDRSSAGTKPATLETGAQI
                     QVPLFINTGDKLKVDSRDGSYLGRVNA"
     gene            complement(22549..23673)
                     /locus_tag="VFI55_01465"
     CDS             complement(22549..23673)
                     /locus_tag="VFI55_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET7072736.1"
                     /translation="MTHSQRRHNLKAQIVAGGLDAMLVTDLVNVRYLSGFSGSNGALL
                     VFADDRDPVLATDGRYRTQAAAQAPDLEIAIERAVGRYLVGRAAEADVGKLGFESNVV
                     TVDGYDALTGELGERSGATELVRAAGTVEALREVKDAGEVALLRLACEAADAALADLV
                     ARGGLRPGRTEREVSRQLEALMLDHGADAVAFETIVAAGAHSAIPHHRPTNAVLAAGD
                     FVKIDFGALVAGYHSDMTRMFVLGKPADWQLEIYQLVAEAQRAGREALRPGAGLREVD
                     GAARRLIDDAGYGDQFGHGLGHGVGLQIHEAPGIGATSAGTLLAGSAVTVEPGVYLPG
                     RGGVRIEDTLVVAGGALTSRDTAGQTPELLTRFPKELAIL"
     gene            23707..24435
                     /locus_tag="VFI55_01470"
     CDS             23707..24435
                     /locus_tag="VFI55_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B-4DMT family transporter"
                     /protein_id="HET7072737.1"
                     /translation="MRNWMLRGLVFAAVMVVVRLLQGALINAFQAQAGLISAVLLLLF
                     VIAVAAWGVVDGRADAAANPDPDRRQDLAMTWLLAGMLAGVVSGAVSWLISLFYDGIY
                     TGGVLNELTTFAAFTALMVFVPAIIGVSIGRWRVDRQFAKQPEKHHGLGVKGERADTD
                     VFSAVRADDTPTGEIPTQTTGARHEERTAAVATAEREEPTQTLPTTPWGAPTKAMPTS
                     DDDANTEVIRTDRHGKPGSEPEKG"
BASE COUNT         3950 a         8298 c         8194 g         4001 t
ORIGIN      
        1 cccgagccga tcaccagcac atggttgagg tcagtccgcc gcggcactag cgcccctccc
       61 ccgccatcag gtcgacgaat tggtcgaaca ggtattccgc gtcgtgcggg ccggcggcgg
      121 cctccggatg gtattgcacc gaaaaagcat gcccgtcaac gagtttgacg ccttccacca
      181 cgccgtcgtt ggcacaggtg tggctgacga tcgccgcgcc gaacggcgtg tcgaagcgct
      241 ggccggcctc cccctcgagc gcgaagccgt ggttctgcgc ggtcacagcg acccgccggg
      301 tcgcatggtc gatgaccggg acgttgatcc cgcgatgccc gaacaccatc ttgtaggtcg
      361 acaggcccag cgcccggccc aggatctgat tgccgaaaca gatgccgaac aacgggatgc
      421 cggcgcccag cacctcacgg gtgagcgcga cgacgtgatc ggcggtagcg gggtcgccgg
      481 ggccgttgga caggaacacg ccgtccggct tgatgtcggc gatctgctcg aaggtcaccg
      541 acgacggcag cacatggctg cgaatcccgc gccgggcaaa gttgcgcggc gtgttggtct
      601 tgatgccgag gtccagggcg gccaccgtga acctcggaaa tcccgccggc ccttccggtt
      661 ccacgacata ggtctccgca gtgctgacct cggcggcgag atcggcgccc agcatcgact
      721 gctggcctcg cacccgctcc accagctcgg ccgggtccgc aagggaggct ccggagaaaa
      781 tccccgcctt catcgagccg cggctgcgca gatgccgcac caccgcacgg gtgtcgatgc
      841 cggcgatccc gacgatgtgc tggcggacca gctcctcgtc cagcgtgccg gtggcccgcc
      901 agttggaggc gcgcggcgac gggtcgcgca cggcgtaacc ggcaacccag atcttgtcgc
      961 cgcggctctc gccgtcctgg ccgttccagc cggtgttgcc gatctgcggc gcggtggcca
     1021 ccacgatctg ccggtgataa ctgggatcgg tcaacgtctc ctgatagccg gacatgccgg
     1081 tggaaaagac cgcttccccc agcgtttggc cgaccttgcc gaacggtttg ccggtgaaga
     1141 cacggctgtc ttccagcacc agtagggcct tattcaccag cgcccctcct cgtcatcgat
     1201 tcgttctgca tcgccgtgag cgcgattcac tcggctccct cctgccggcc gtccggccag
     1261 ccttccaacc aaccgccgta ttcgccgcgg ttatctgcac gaaagcccgt gtcgatctcg
     1321 acgcccgacg gcagccgcca ccggatcgcc aggatgccat tgcgggcagc gactttaccg
     1381 gccactccgc gctcggtacg gacggcggtg atctcttctt gcggaatcca aattggctta
     1441 gcacgaatgc gttccagcag gatgccctcg ggaaagcggg tcagcaccgc cttgctgcgg
     1501 tagcccagat cgccggccgt gatgcgctcg ttccaggcgg gcgacagcgt ggacccgaca
     1561 tacaagccgc ggatcgtggt ggtcgcggag cccaccgcgt cgggcacgtc gggcaggtca
     1621 ccgatcagtt ccgcctgccg ttggccgcgg cggtgccacc cgcgcatcat cagttggatc
     1681 agcaccgcga tcaccaccac cagcaccgcc gcgaagatca gcgaccccac cagcgtgccc
     1741 gagttcatgc gcgccgctcc tcctcatcgc tccgctctgc atcgtcgccg gcgcggttca
     1801 tgccgagatc ttcccgtccc gggctgtgac cttcccgcgc agcagggtcg ccgtcacggt
     1861 ggcgggcaac gtcatcgact cgtatggcgt gttggcggac cggctggcca gatccgaccc
     1921 ggcgaccgtc caggtggcct ccgggtccac gacggtcagg ttggccggtt cccccacttc
     1981 cagcggccgg ccgtgatcgg gcaaaccaac gattcgcgcc ggattctcac tcatcaccct
     2041 ggcgacatcg cgccaggtca acagtccggg cgccaccatc gtctgcacca ccaccgacag
     2101 cgccgtctgc agccccagca tgccggggcg cgccgcggag aactccacgc atttctcgtg
     2161 ctcggcatgc ggggcatggt cggtggccac gcagtcgatg actccctcgg ccaacgcgtg
     2221 acgcaacgcg atggcgtcgg cggcctcgcg cagcggcggg ttgacccggt tcaccccgtc
     2281 gtagttggcc agcctgctgt cgtcgagcag caggtgatgc ggcgtgactt cggcggttat
     2341 cgagatacct tgcgccttag cccatttgag gatctcgacg gttcccgcgg tggaggcgtg
     2401 gcagatgtgg acgcgggcgc cggcgtcgcg ggccagcagc gcatcgcgcg cgacgatcga
     2461 ctcctcggcg gcccgcggcc atcccgccag tcccagcctg gcggcggtgg gtccctcgtg
     2521 ggcgacggcc ccgacggtga gcctcggctc ctcggcgtgc tgggcgatca gcgcccccag
     2581 gccggtggcg tattccagag cgcggcgcat catcagcggg tcgtgcacgc agatgccgtc
     2641 gtcggagaac atccgcacct gcgcggcgcc ggccgccatc atgcccatct cggtgagcac
     2701 ggccccggcc agcccgacgg tgacggcccc gacggggtgc acgtcgacca ggccgacctg
     2761 ctggccgcgg tgccagacgt ggtcggttac caccgggctg tcggcgaccg gattggtgtt
     2821 ggccatcgcg aacaccgcgg tgtatccgcc caaagccgca gccgccgaac cggtttcgat
     2881 gtcctcggcg tattcgcggc cgggttcgcg caggtgggtg tgcaggtcca cgaagccggg
     2941 cagcagcacg tgcccggggg catcgatgac gtccgcatga tcggggatgg caagacccgg
     3001 gccgatttcg gcgatctggc cctctcgccc aggagcggag accagcacgt cgacccggtc
     3061 accttcgccg tacagcctga ccccgcgtag cagcacgctc atgcggcacc ctcgttttgc
     3121 gccgattcgg tgccgacgag cacatggaag agcaccgcca tccgcacgtg cacgccattg
     3181 gaaacctgtt gcagcacagc ggattgcgac gagtcggcga ccgacgacgc gatctccatg
     3241 ccccgcacca tcggccccgg gtgcagcacc accgcgtggc ccggcagcat cgcctggcgt
     3301 cggcccgaaa gcccgtagcg aaccgagtac tcgcgcaccg acgggaagaa tccgccgttc
     3361 attcgttcgg cctgcacccg cagcatcagt accgcgtcgg cggccggcag ctccgcgtcg
     3421 aagtcatggg tgacggtgac cggccactcc tcgaccccga ccggcagcag cgtcggcggc
     3481 gcgaccagca ccacctcggc gcccagggtg tccagcagca tgacgttgga gcgggccacc
     3541 cggctgtgca ggatgtcgcc gacgatcacg atgcgccggc cttcgatgcc accgagccgc
     3601 tggcggatgg tcaacgcgtc cagcagcgcc tgcgtcgggt gctcgtgtgt gccgtcgccc
     3661 gcgttgatca ccgacggccc gagaccccgg gagtcaccga ccgcaaaaga cgaagtccag
     3721 tcggccagca gatgggcggc accggatgcc gggtggcgga tgatcagcgc gtcagctccc
     3781 gcagcccgca acgtcaatgc ggtgtcgcgc agcgactcgc ccttgcccac cgacgatccg
     3841 gccgcgctga cgttgatcac gtcggcgctc atccacttgc cggccacctc gaacgacacc
     3901 cgtgtgcggg tcgagttctc gtagaacatc gtgacgatgg tgcggccgcg cagcgtcggc
     3961 agcttcttga tctcgcggcc caccagcgcc tgcgcgaacc cgtcggcgtc gtcgaggatc
     4021 gcggtggcca cctcgcggct caggtcagcg gcggccagca gatgcctggt cacgagtcac
     4081 cgtctcgagt gatcacgacg ccgtcgcagc cgtcgtgctc gcgcagttgc acgtgcacgc
     4141 tctcgctgcg tgaggtcggt acgttcttgc cgacatagtc ggcgcgcaaa ggcagttcgc
     4201 ggtggccgcg gtcgaccagc accgccagtt gcactgcccg cggccggccc acgtcacgca
     4261 gtgcatccag ggctgagcgc accgagcgcc cggagtacag cacatcgtcg accaggatca
     4321 ccagcgcgtc ttcgacgcca ccggcgggaa tcgacgtggc cgccagcggg cggggcggct
     4381 tcttcatcag atcgtcgcgg tagagggtga tgtcgagggc cccgtggccg acgtcgaccc
     4441 cgctgtattc gcttatgttg acggtcaggc ggttggccag gatcacgcca cgggtcggga
     4501 tgcccagcag caccacccgt ggcgcgtcgg gaccatccag cgcggtcttt tcgatgatct
     4561 gatgtgcgat gcgggaaatg gtgcggccga cgtcggccgc cgacatcagt tctcgggatc
     4621 cgcggcctaa gccggtgtca cccgctgcac ccatacgaaa cttgacctcc ttctccgcct
     4681 cgccggacgg atcgttaaag gatgtcgact gcccggcagc gtagcacttc gcaccgccgg
     4741 gctcgcgctg cggtcaatgt gtcggtggcc tgacctagca tcgccaatat gttcgatttg
     4801 agagagcttg ctgcggtgga tccgcatgcc gacgaggccg ggttgatagc gcggatcacc
     4861 gagttggagc gggcgaagtc ggccgccgcc gcaggtcagg cgcgagcggc ggcggcgctg
     4921 gatgcgctgc gccgttccaa cgaggccgac gccgatgtgc cggctgcgca ccgaggccgt
     4981 ggggtgtcca gtgagattgc gctggcgcga cgggattctc cggctcgggg tgggcggcac
     5041 ctcggattcg cgaaggcgtt ggtgtacgag atgccgcgca cgctcgccgc gctggaagcc
     5101 gggctgttgt cggagtggcg ggccacgctg atcgtccggg aatcggcttg cctggacgtt
     5161 gacgatcgcc atgagctgga cgccgagctc tgcgccgacg agtcgaagct ggacgggatg
     5221 ggtgatgcgc ggatcgccgc gacggcccgg gcgatcgcct accggctcaa tgcgcaggcc
     5281 gtggtggatc gcgccgccaa ggccgcatcc gagcgcacgg tgaccattcg gccggccccc
     5341 gacaccatga cctacgtgac ggcgctgctc ccggtggccc aaggtgtgtc ggtgtatgcg
     5401 gcgctcaaga gagccgccga caccacattc gatgaccgca cacgtggtca ggtgatggcc
     5461 gacacgttgg tcgaacgcgt caccggacgg cccgccgacg tagcccaacc ggtcgctgtc
     5521 aacctgatca tcgccgacga gacgctgctg ggtggggatg acatcgcggc ggtggttgac
     5581 gggtatggcc caatcccggc ggccgtcgcc cgcaacctgg tgagcaacgc gaccaccgat
     5641 aagcggtcgc aagcgacgct gcgcaggctt taccggcatc cgcgctcggg ggcgctgcta
     5701 gccatggagt cgcgggcgcg ccgtttcccg aaggggctgg ccaccttcat tgggttgcgg
     5761 gatcagcgct gtcgcacccc ctactgtgat gccccggtcc gccaccgcga ccacgcccag
     5821 ccgcgccatc gcggcggtgc gaccaacgcc gccaacgggc tcgggttgtg tgagcgctgt
     5881 aactatgtca aggaagcacc cggctggcgc gtcaccgccg caatggacga aaccggcagg
     5941 cacatggccg aattcactac tcccaccggg atgcgttacc agtccggcgc gcctcccgca
     6001 cccgggccac tcgagatatt cgtcagtcag gtagagaccc ggatcggtgt ggcactcgcc
     6061 aaacttcacg ccgcttaggc tggcggaaat aacaggtgtt agctgcctct aggtgcaaag
     6121 atattgcccg ttgagcagac aattcgacgg tggcgagtag gaaccggtaa gcacgcctct
     6181 tagaccacct gtggctgaac caccgggtgc aatggtgcga ttccaattcg cgggggtgag
     6241 gacgtagtgc gtgccagatt gcgtaagggt gctattccac gtgtgcgaga caccttgtcc
     6301 caccggcatg tcgaattcaa gcttccaatc ggtgagcggc gccatgctcg agttgatgat
     6361 ggtgaagtgg gcgatgaaac cggtctgcca cgtatgttcc accgacaacg tcgccgcggc
     6421 cgcagccgca tgagctacgg gggtgatggc gagcccgagg atggcaacta tcaatgccga
     6481 cacggttacg tgaagcgctg tgcgccagcg cttcacgtaa ttgttcagtc cggtcatagg
     6541 agccaacact aaagccgaac aggcgatatc ggcccacgca tcgcgggtca gctgccgtaa
     6601 ctttgctcaa ccgaaaccgt catgaaattt tagcctcgat ccaccgatac ccgcttttga
     6661 tctgtgcggt tagtcgctgc gaaggccgta ggtgcagcgt ggaggatgct atgacgcatg
     6721 tcccactggc tggacgacga cggcaagcac agctacgccg ccgccgcaca tcgggctagc
     6781 aggcgcggtg ccaccctgtt gctcagctgc ttctccaacg ccaacccctc cgacgacgaa
     6841 tggtcacgac cggaggtatc cgagcaaacc gtgcgcgacg tcctcggcgg tgcgggctgg
     6901 gacgtcgaat tgctggagcc cgccgggtgg cgccgcgaaa tcggcggcaa ggatgtcgaa
     6961 atggcgttct ggcacgtccg cgcgcagtgc gctagtggaa cgtgcctgaa ctactcggcg
     7021 tgtggcgttt tcgaagcgca gaagcaactg ggcagcattc cggctgttag ccgacgcggt
     7081 aaccagctgg ctgcactacg tatgatttgg atattcacag ccggagtcgt ctattgccaa
     7141 cccatttggg cgtgagaaag catgggaatt atgaccgtcg ttcgtaccga actggctcgg
     7201 cagttttact ttggcgacga cgcggtgttg ttggtcatgg acaccggcgg ggtggcgact
     7261 ttcctctcgc cgttgctttc tgcgcaccaa cagggagcgt cgcggctgga gcacggcggg
     7321 acggttcacg atttcctgat tgagccaggt gccgccgaca tcgagctgca cggtgatcgc
     7381 gtggtgtggc ggctcgatca tgccaaggcc gcagagatca tcgagttgtt gacggcgatg
     7441 acccagagca gcactggtgc gggtcattac tacgtcgaca tctcgacacc ggctgagacg
     7501 ctggtgttgt cacgcaacga acatgtctag ccgacaggcc ccgtgcggct gactgacctc
     7561 ccgtgcaggg cttccggtcg gcaatcatct gctcgcgcag tatcggtcgc gctcccgtaa
     7621 ggcatgatcg gcagctgacg ctgagacctt ttggcaagca acagctatga gggcgcgcgc
     7681 tactcgaagt cccgggcgcc ttccgccgga tcatcttctc ccaggatggg gaaatgatgg
     7741 gggatgctgt gcggcgggtg aatgacatgg ggccctaacg gcgtggcggg tccgcccggg
     7801 attatcggca tcccgccgct ttctggcggt actggcctcg ccggcggtgc ttcaaccggt
     7861 ggcggtgctg gacggggcgc aggttggggc ttagaggccg gcgacggggc gggtccaggc
     7921 acgggcgcct tgggcgctgc tttagctgtg tgcggcggaa gttcgcccat tgggatgttc
     7981 tgccattggg agaacttggt gtcgtgtggg ttgtagtccc agtgcgggtt gtgccactgg
     8041 ttggcgcctg ggtcatatcg ccactcaccg cccttgtcgt cccacagact caggccgccc
     8101 ttggcctctt cgctggggcg actggccggt cctaccgtca ggttcccctc gaccggagga
     8161 tgaacgccgt cgggtggcaa accgcgagcc ggcggaggcg gaggatcggg ttccggattc
     8221 ggcgcgtcct tgaaggtgtg gcggtcgacg gcctggacgc cggatccctc gtggaaattg
     8281 aggacgtcga tgtccttggt agcggcggtg attcgcgtgg tgagctgctg atcgctggtg
     8341 agtagggcgg ccaaccggtg acgaatgaag ctggcgtgtc cttgagccgc agcaagacgt
     8401 gcggcccgaa actcgggcgt gccgccacgc aaccggtcgg tgaccgaata gtcctctccg
     8461 acatgaaatc cgtcgacgcg cgcatcgttt acggcctcta gcactccgtc tttgcacgcc
     8521 tgcaactgct ggtctccgcg aagagcgagg ccggccgcgc catgcaactg gtcagacgcc
     8581 tcgcggacct tcaccaaatc gaggtgggag cgttcctgcg cggcagccgc ggcccgaccc
     8641 ttccacgggg agcccccggg gatcgacatc gtgtcatgaa catcggcgaa ggcctgctcc
     8701 cacaggttgg ccgtccgcgt ccaatactcc gccgccccac gcaaatacgc ggtatccaag
     8761 gtctgcactt gcgacagcgt cggcagcgtg gcggtcgccg ccatctaaaa caccgtcacg
     8821 ggccgcagcg cccgcatcag ggccgccgag ctggcttcat tcgagccata gccggccgag
     8881 gctgcggcca gtttgccggc cgtggtttgc atccgagtcg cacaccgcat gccggccgcg
     8941 acgacctgcg cgtggctgat gttcaccgcg gccgagctgg ccagcggtga cgatcccgcg
     9001 ccggccggag cgctattggt cgccagcctg gtggccaggg cctcacacca gttggcctgc
     9061 gcgcgcagcc caccctcgct gacctgcagc tcccccgcgg tccgccccga ctccatgcgc
     9121 agactctaaa accgacgccc gttgcgcaca cttcaccagg gagttatcgc aagcccgtcg
     9181 cgcccgcccg ctaacctgaa ccgggtgaat cttgacggca atcaggccgc tatccgcgag
     9241 gtctgtgagg ccggcctact ggccggtgcg gtgacggtgg tctggcagcg cggagaagtg
     9301 ctgcagctca acgagatcgg ctaccgagac attgacgcgg gcctgccgat gaccagagac
     9361 acgctgttcc gcatcgcatc gatgtccaag cccgtcaccg tcgcggcggt gatgagcctg
     9421 gtcgaccagg gcaagctggc gctgaaagac ccgatcctgc gctgggcgcc cgagttggcc
     9481 gacgtgcagg tgctcgacga tcctcatggt ccgctggacc ggacgcatcc ggcccgtcgc
     9541 gccatcctca tcgaggatct gctcacccac accagcggcc tggcctacgc attctcggtg
     9601 tcggggccga tctcgaaggc ctacatgcgg ctgccgttca accagggccc ggacgtctgg
     9661 ctgagcgagt tggccaagct gccattggta catcagcccg gcgagcgggt cacctacagc
     9721 cactccatcg agctgctggg catcatcgcg gctcgcgtcg aggacaaacc gttccaccag
     9781 gttctcgacg agcgggtgct ggacccgacc ggcatgcccg acaccggatt cttcgtttcc
     9841 gtcgaagcgc gccggcgcgc ggcgaccatg tactcgctca ccgaggaaca cgtcctacag
     9901 cacggcgtga tggggccgcc gcacatcaca ccgccatcgt tctgcaacgc cgggggtggc
     9961 ctgtggtcca cggccgacga ctacctgcgg ttcgtgcgga tgctgctggg cgacggctcc
    10021 gtcgacggcg tgcgggtgct ctcccccgag tcggctcgtc tcatgaagac cgaccggctc
    10081 accgacgaac agaagcggca caacttcctc ggagcaccgt attgggtggg ccgcggattc
    10141 gggctcaacc tgtcggtggt gaccgatccg gcgaagtccg ccccactgtt cggaccgggc
    10201 gggctgggga cgttcagctg gcccggcgcc tacgggacgt ggtggcaggc cgacccgacc
    10261 gcggacctgg tcctgctgta cctgattcag aatctgcccg acctgaccgt cgacgcggcc
    10321 acggccgtcg cgggcaacac gtcgctggcg aaactccgca cggcacaacc gaagttcgtc
    10381 cgccacagct atcgcgcggt cgggctctag caccatggtc gattacccac cagtccgcat
    10441 catcgggccg cggttggtgc tgcgcccgcc cgtactcgat gacgccggcg cgctcttcca
    10501 gcgggtcgcc cgcgatcccg aggtcaccag gtatctgctg tgggcaccgc attccgatgt
    10561 gggggtgacg cggcgggtga tcgccgagaa gatgaatgtc agcgacgacg agcggacctg
    10621 ggtcatcgag ctgaagcaca ggggcgaagt gatcgggctg accagttgcc ggcgcaagtt
    10681 gccgcactcg gtcgagatcg gttactgcct cggtcggcgc tggtggggca agggtttgat
    10741 ggccgaggcg ctcgacatgc tgctggatgc gctggcgggc gatcggctgg tgcatcgggt
    10801 ctgggcaacg tgcagccctt acaacgagcg ctcggcgcga ttgctggagg gcgccggttt
    10861 cgtcctggag gggcggctcg cccaccacgc cgtctacccc accatgggac ccgagccgca
    10921 ggacagcctg ctctacgcga aggccctgcg gtgagcgttg gccgccacgc atgaccaagc
    10981 cggggcggct acccgccttg ttccaaggct cgacgcgcgt aggcccgcac gtcggcgtcg
    11041 ctgtccttga gtgccgttcc gagcgcgtcg cgggcagcgg gttcaccggc ccatcgggtc
    11101 aggctcagca cggccgcctt gcgcacgtcg aggtgcgggt cacccagagc cgcgaagagg
    11161 cccgaaaccg ccggcgccgc aagttcttcc gaggcacccg acagcgcacg ggccgccccc
    11221 tcgcgcacct gccacgccgg tgcccgcagc gccctttcga ttgccgcgaa atcgtcttga
    11281 cggcagccta attcccccag cgccgccagc gcggccgcgc gcaccagcgg atcggggtcg
    11341 gcgatcagcg cgctgactgc ttgcgcgccg tcccgcagcg tggcaaggcc ggcggccgcc
    11401 gcgatgcgca cctcccggct ctcgtcgcgg gtggcttcga cgaccccggc gacgtcgtcg
    11461 accgacacca gcgcccgcac cgcctcgatc cgcacccggt ggtcggcatc ggtgagcgcg
    11521 ccgcggtatt cccccgcgtc accggcccgc cgcgcgtaaa gcaggtagac ggcggccgcg
    11581 cgcaccaccg tgtcgcggga acccagatac gccctggcgt cggccgggtc gggcagcacc
    11641 tcgaccagct cgcggattcc ctcggcggcc gtgccgcgca ccgcggcgtc ggcgtcgtcc
    11701 agcacggcga acagggcggg cgcgtacccg tcgggaatgt gttcggtcag cgtggccacc
    11761 gcggtgcggc gcaccccggg atcggagtcg gccagatacg gccccagatc ggcgatcgtc
    11821 ggctcctcga gggcgaggac cttggcgatg cgcggggacg gcggctcgaa cgtggttgcg
    11881 gccgaggcga ctcgggaccg ggtggtggcc ggcgcctggc cgcccaccag cggcggctgc
    11941 tctaccgcct gcacggtctg gtccgacggc ggcaggccgt ccagctccgg caccggcacg
    12001 aaatacgggg ccaccggccg cttgaggaag accatctccc cgtcggtccc cttgcgcagg
    12061 ttgaggtgat agccccattg gctgtcgtcg cggaccggca ggtcggcgcg gtcgtggtag
    12121 aggccccaac gcgattcggt ccgggtgagc gaggatcgcg cggccatctc ggcgcagtcc
    12181 cggatgaacc acacctcgac ggcgcgcatc agctcatgcg gattgcgggc acccatgtcg
    12241 gcgatctcgg cttgcatccg gtcgaaggtg cgcaccgcga tggacagttt ggccgccgtc
    12301 ttgggcggcg cgacatagtc gttgacgaat cgccgcaatt tgtattcgac ctgtggctgc
    12361 ggcggtccgt ccggatgccg cagcggccgg tagatcagct cgtgtgcggc acgcagctgg
    12421 tcttccggga gtgtttgtgg ggcagcgact tcctcgcatg tcgacgcggc gtcggtgccg
    12481 gccaggtcgc cgaagacgaa tgccccgatc atgtaattgt gcgggacaca ggccaggtct
    12541 ccggcggcgt acagtccggg gacggtcgtg cgggcgtgct cgtcgaccca gactcccgac
    12601 gcggaatgtc cgctgcacaa accgatttcg gagatatgca tctccacgtc gtgagtacgg
    12661 taatcgtggc cgcggttggc gtggaacgtt ccgcgggtcg gccgttcggt agtgtgcagg
    12721 atgttctcca gcgcggtcag cgtctcgtcg gacaagtgag acaccttgag gtagatgggc
    12781 ccgcgggcgg agtcgatctc gcttttgact tcggccatca tctggcccga ccaatagtcg
    12841 gagtcgacga accgctcgcc gtgcgagttg acctgatagc cgccgaacgg attggccacg
    12901 tacgcgcacg ccgggccgtt gtagtccttg atcagcgggt tgacctggaa gcattcgatg
    12961 ccggacagct ccgcccctgc gtggtaggcc atcgcgtagc cgtcgccggc gttggtcggg
    13021 ttctcgtagg tgccgtagag atagcctgac gcgggcaggc cgagccgccc gcacgccccg
    13081 gtcgacagga tcaccgcctt ggcgcccacg gcgacgaatt cgccggtccg cgtgttgaat
    13141 gccgcggcgc ccactgcgcg gccgtcttga gaagcatccg tgagcacccg caccggcatc
    13201 aagcggttct cgatgcggat cttctcccgc atcgagcgct gccgcagcac ccggtagagc
    13261 gccttcttga cgtccttgcc ctccggcatg ggcagcacgt aggagcccga gcgatgcacg
    13321 cgacgcaccg catactcgcc gtgctcgtcc ttctcgaatt tcacgccgta gcgctccagc
    13381 cgctgcacca tggcaaaccc tcgggtcgcc gtctgataca ccgtgcgctg attgacgatt
    13441 ccgtcgttgg cccgggtgat ctcggcgacg tagtcctcgg gttcggcctt gccggggatg
    13501 acggcgttgt tcaccccgtc catgcccatc gccagcgcac cggagtgccg cacgtgggcc
    13561 ttctccagca gcagcacctg ggcgccccgc tcggcggcgg acagggccgc catggtgccc
    13621 gcggtgccgc caccgatgac cagtacgtca cagtcgagcc ggagcggcgc cagatcgggc
    13681 atctgcatca tgccgccacc gccgaatgat caagcgccgc aatgatttca gcacgcagcg
    13741 cgggccgttg ccgatcgctg cgcggctccg ccacgtcgat cagggcgcgc agcggccggc
    13801 cggcgttgcc cagcacggct atccggtcac cgagcagcag cgcctcgtcg acgtcgtgcg
    13861 tgacgaagac gatcgtggtc gggtgcgcac gccaggtatc gatcagcaat cgctgcatgg
    13921 cggagcgggt ttgggtgtcc agggcaccga acggctcgtc catcatcacc gcgcgcggcg
    13981 ccccggccag gccgcgggcc agctggacac gctgccgcat gccgccggac aggctcttgg
    14041 gtagatagtc gccgaagccg gtgagcccga gttcggctat ccaccggtcc gcctccgtgc
    14101 gccgtccggc gcgcggcacg ccgcgcagcc gcagcgccag ttcgatgttg gatcgcacac
    14161 tgcgccacgg cagcagcgcg ttgtcctgaa acaccatgcc ccggtcgccg gaggtggtgg
    14221 tcacttcggc gccgtcggcc agcaccctgc cgtcgtcggg tgacagcagg cccgccaggg
    14281 cccgcagcac cgtggacttg ccgcatcccg acggcccggt gagcaccagg atctcccccg
    14341 gccgcgccac caggctcagt ccctcgatca ccggaactcc ggtgtaggac aaccgaactc
    14401 gatccagttc gaggcttact cccacggtca tcgctgctcc tcctccgcgc gcggcaacca
    14461 gcgggtcacc cgccggccga ccacttcgac ggcggcggcc gtcacgaacc ccagcacgcc
    14521 gatggtgatg atcccgacga acacctgcgg gtaggacaac accgtgtagt cctgccaggt
    14581 gcggtagccg acaccgagcc ggccggagat catctccgcc gagatcacac agatccacgc
    14641 cacccccatg ccaaccgaca ggccgccgaa cacgccgggc aggatgcccg gcagcaccac
    14701 ctgggcaagc acctcccacc ggccgccacc cagggtgcgc actgcgtcct cccacaacgt
    14761 gggcaacgcc cgcacggcgt gccgggtgct gaccatgatc gggaaaaagg ccgccaggaa
    14821 ggtgatgaac acgatgccgg cctcgtcggt gggaaacagc aggatcgcca ccggcaccat
    14881 cgcaatggcc gggatgggcc gcgccagctc ggtcagcggc ccgacgacgt cggcgaagag
    14941 gcgggagcgt ccgaggagca cacccgtcgc gacgccgacc accgcggcta ttccgaagcc
    15001 ggtgaggatg cgcagcaacg attgggccag gtcgagccag tactcgtagg tctgcacccg
    15061 gcgggtcagc gcatgcgcga tctgggtgac ggtcggcagc gtgtcgaacc gcagccaaaa
    15121 ccgcaccttt ccggcggtga gcacctgcca gagcccgatc gcggcggcca ccgacgccaa
    15181 ccgcagcagc cgggaccgcc acggcgaggc ggcttgtcgc ggcgtgccga gaatgggcgc
    15241 cgatacggtt tggtcgatca gttgggccgt catagcgaac ctcccagtgc ctgttggtaa
    15301 ttcacggcaa cgccgcccgg atgcgcggcg agatagcgtc gcgcgccggc cagggttccg
    15361 aaggggcgat aggactggcc gtcctgcacc cagaacgcct tgtcggcgaa ccagcgggtg
    15421 cccagctcgg catcggggac ataagccgca cggactttgg cgccgtgggc gatcgcgtcc
    15481 ctgacggccc gcagcaggct ggtggggttc gcgagcgttt gagttgtgtc ggatccatcc
    15541 agccacagct cactcgcgag ggcgggatca ccaccgagca acgacgggtt cgcggtggct
    15601 gcgcgggccg cgtcatagtc caacccgcgg gcgccgaaca cggtgcgcag cggcccgtcc
    15661 tggacgaacc cgctgacgtc gaggtcggcg aaatcgccga tcgacttcag gtatggcaca
    15721 tcgtttttca gcgcgtcgac cagcgacggc ttcagcgtgg tgtcgaatga cgtgccgccg
    15781 ggaccgttgt agaggtacac cacctcctgc ggcagcccgc ttgcttgcgc gacgatgcgg
    15841 gcggcctgaa gcggcttctg gttgaggaaa tcggtggcgt ccagttgcgc ttgcagaaac
    15901 gccgcgagca cttcggggtg cgaggccgcg taggtgcggc gcaccaccac accgtgcagg
    15961 gtcggcaggt tcagttccgc gccgtcgtac agcagcttgg ctttaccctg gtagaccagc
    16021 agcccgggcc acgcgacgaa ctgcgaaaga ccttgcacct gaccagattc cagtgccgac
    16081 gcgccgacct gcggctgctg gttgagcacc tgcacgccct tgatcccagc cctgtccagg
    16141 gcgcgcacca gcgtgccgtg cccggccgaa ccgacactgg ccgaaacttt cgcacccgca
    16201 aggtcgctca ggccggtcgc cgtcgaatcc ggctccacca ccaccatgtt cagcgcaccc
    16261 ttggggttgt aaccggtgac ggagatcatc tcggtgtggg ccagcgggtt ggcctgggtc
    16321 ttggaaccgt tgatcaacat ggggtaatcc cccatggagc cgatgtcgat cttctcggcg
    16381 agcatctggg cggtgatcgg agcgccggtg tcgtaatcct gccatcgcac cgagtatttg
    16441 gtgccggtgc gggtggtgat gtcggcgagc cggcgctcca ggtagccctg ggcgcgcagc
    16501 agggtgcccg cggtgacggt gttgatggtc ttcgactggt atccgatgac gacgttgacc
    16561 acgccggagg attccgaaag cgattccagc gagcagcccg aggtgagcga ggcgacagcg
    16621 atcaccacgg acaacagccg cgcgatgtgg cgtttcatga aaaaccctag gggagaagtc
    16681 ttttcagcgg atgagatacg gcatgttgac cgtgacggcg ccagttgggc agcgggccgc
    16741 gcagggcccg cagtaccagc attcgtcgac gtgcatgaag gccttgccgg tgtcggggtt
    16801 gatggccagc gcgtccagcg gacagacttc gacgcacagg gtgcagccgt cgatgcacag
    16861 cgactcgtcg atcgtcaccg gcacgtcggc acgattattg accagcgtca cggcttgctc
    16921 cttatcggct cggccctaat cagactgccc cgcatggtga ttcgatcgcc ccgcatccgg
    16981 atgtactcca aatcaacggg tctgccgtcg tcgaggctgg tgagtcgctc cagcatgagc
    17041 agcgccgccc cgtcgggcac ctccagcgtg gcggccgagt gcgcgtcggc ggggatggcc
    17101 tccagcgcca gcgacgccga gcccagccgt tgtccgctca cctgctcgat gagcgcgaac
    17161 aggtcgttcg tctccagcgg gtagtcgagg atttgcgttc cgatatccgg ggccaggtac
    17221 gtcaggtcga ggctgagcgg caagtcgccg aggtaacgca accgctccac gaagaccgcc
    17281 tgctcgccgg gttccagccg cagccggcga gcgaccgacg gcggggcggt caccagcatg
    17341 gcggcgcgca cctcgttgcg gacctccccg aggtttttga acgtttcctt cagccccagc
    17401 agcgcatcga gtccgtgctc gtacttgcgc tgcgcgacgt gggtgccgac cttgggcccg
    17461 cgctcgatca gcccttcgcg tttcaggacg gcgagcgcct cgcggacggc attgcgggag
    17521 acgaagaatt ccgccgccag ctcctgctcg gccggtagcc cgtcggggta tgcaccggcg
    17581 tggatctgct ggcgcagcac gtcggcgacc cggcgcgcac ggtcggcgcg aggtcctgcg
    17641 atcggtactg gttgcgccac gacgtcacgc taacgcagct cagaggccta ttcggggtgg
    17701 ccggtggggg cgtgcaacgg cgcgacgggg tattgcgccg ggctgggttc ggcgccaacc
    17761 tggcaaaacc cgggcccgtc gcgctattgc gccacctgcg cggcgacgcc gcctttgcga
    17821 tcccggtgag ccgaacctcc gtcggcagaa gcctgatcac atccgccgca tgggctagat
    17881 gggcctcatg ggcctctccg gaactcggtt gacaatttgc ccacctcaca gcgactttgc
    17941 ctgctcgccc gccaaccctt cgaatttgtc gctcgcaggt gggcaaaaag tgtgtcgcct
    18001 ccagcagaga cgcgtgtggc gaatgtgacc accgtcagtt ggtcagcgag ctaaggcccc
    18061 tcaaccgtcg ttggcggccg tccgcaccga gcgatctcca ttgacggctc ccaaggcccc
    18121 caagctggcg gcatccgatg agagttcgaa gatcgggacc gccgcaccca ccgcggcggt
    18181 tgccgcatta cgttgggcct ccatgcgggc cagcgcagcc tgggtgaaca ccgacaaccc
    18241 gaggtcgtgg ttgtacccat ggatttcctt gacgtccagc tgggcgagga aatagacgat
    18301 ctccttggac accacctgac cgggtaccag caccgggaat ccgggcgggt agggcaccac
    18361 gaaggtggtg gacaccagcc gcttcccatc ggcgagccgc cgcccggccg cgccgatcag
    18421 cacgtattcg cggtcggtct cctcgtatcc ggcgtagaag gctgcccgca tgtcaccgac
    18481 tgagcaagcg tccacggggc gaaaggcaga gtcgaactcg ctgaagtcgg gcaggtgcgg
    18541 caggtcctgg gtgatctcct cgacccggcg ctggtgcagt gcccagtcgg ccgcgctggc
    18601 gccctgcttc tcccggtcga aatcggcggc cacccgccgc agcacgtcga gcagatggtg
    18661 cacgctcgac cacgtgacac cgatggtgaa gatcaacaac acgctgttga tcgacgtctt
    18721 gttgatctga atgccgaatc ggtccatcag gatcttctcg cggaagtcgt acccgttcat
    18781 gccggtgttg ccgaggaaca gcgtcacccg cgtcgggtcc agcacgaact ggtcggatcg
    18841 ccaggcctcg ttccactcgg ccagcgcgcc ctgcctgacc tcgcggtagg agctgaccgc
    18901 cgaggcccgg aactcctcgg gcaccaggtc ggactcgtcg aggatgcgga accacttgct
    18961 gatcaggcgg tctttgcgga cgcggtgccg gaagaccagc gccatgtcgt agacctgccg
    19021 gaccagctgg aagccctcca agtcgacctg ccggcgggcc aggtccagcg aggcgagaag
    19081 ttgctggttc ggcgacgtcg aggtgtgggt caagaacgcc tcaccgaacg cgtcgcgggc
    19141 acgcgagttg aagtcctggt cgcgcacgtg gaccatcgac gcctgccgaa acgccgatag
    19201 cgacttgtgg gtcgagtgcg tcgcatacac ccggacccgc gcccgaccgg ggtcgggcag
    19261 cagccggtgg tcggcccact ccgaccgcgg gatcccctgc atcgacgcgg cccaattccg
    19321 gtattcctca gcgtattcgg gcgacgccag cgtctgttcg aggcgctccg cggacaccat
    19381 ggcggtgcgc tgccgggccc acggcacggc ggtggcgaac gcgtaccacg cctcgtccca
    19441 caggaagcag atgtccggct tgattgccag cacctcctcc atgacacggc gggggttgta
    19501 cacgacgccg tcgaagatgc agttggtgag cagcagcatg cggacccggt gcaactgccc
    19561 ggccgcctcc aggtccagca gcgccttctt gacggtgcgc agcggcaccg ctccgtagat
    19621 cgcgaactgc ggcaacggat acgcgtccag gtacagcggg tacgcgccgg ccagcaccag
    19681 gccgtagtgg tgcgacttgt ggcagttgcg gtcgatcagc acgatgtcgc ccggccgggt
    19741 cagggactgg acgacgatct tgttggcggt cgacgttccg ttggtgacga agtaggtgtg
    19801 attggaattc caggtgatcg cggccttgtc catcgctttc ttgatgttgc cgtgcgggtc
    19861 cagcagcgaa tccaggccac ccgaagtggt cgaggtctcg gccatgaaga tgttgcggcc
    19921 gtagaactca cccatgtcct gcagcgactt cgagttgaag atgctggcgc cacgcgcgac
    19981 gggaagcgca tggaactggc cgaccggagc cgacgcatag gcgcgcaggg catcgaaaaa
    20041 cggtgtggcg aaacggttcc gcaagccgga gatcaccgtg ctgtgcagat cggtgacgtc
    20101 gttgagacgg tagaaagtcc ggtcgtagac gtcgggctcg tcgtcgttgc cggcggcgat
    20161 cgactcgtcg gtgagcaggt agaggtcgat gtgtggccgc aactccttga tccactcgcc
    20221 gcattcgacc cagtcctgcg cgcagtcggg gctgaccgcc tccgagtcgt tgggccccaa
    20281 cagcgtgttc atcagcggca cccgatcccg cgagcgcagc ggcaggtcgt ggcggatgat
    20341 cgcggcctgg atctcgccgt tgagtgcgac cgcggtgatg gcatcctcga cactgggcgc
    20401 caccaggacc tcgaactgga cgtcttcggc cgggttgcgc agtgcgcgca ggctttccgc
    20461 caggcaatcg ggcgccgtcg gcggcacgtc gtcggcgacc aacaccgtgt agaactgctg
    20521 ctgtttggcc tgggccacca gctcctggtc gtccagcggc gcggacgtgt cgaaaagcgc
    20581 tgcgcggtcg ccgtattcac tcaacaggcg caccgccagc gagacctctt cggtcagccg
    20641 caccgtggaa aggctttcca ggtgcgcgcg gaagatcgcc agattgtccg caccgggata
    20701 cagccagtac cgctcgtagg caccgatgcg gtccatcaga cgcttcaccc gggcaacatc
    20761 atggcctttg tccagcccgg cgaggtcgac ctcggcgagg tgccggcatg cgtcgtcgag
    20821 cagattccag gtgtccaggc gcgtgtacga cgggttggcc accgccgcca gagcggacac
    20881 acgaagtcgt cgagggcggg cactgtttcg attcatatcg ttacctgcct gtcgttctgg
    20941 cgtcagtgca actcaccatt atcccgcgca cgacggaaac gcagacagcc cgggggcgca
    21001 cgttcgccat tgtgtcccgt tggcggacac cgcgggatca tttgaccgag tcggtcagca
    21061 ctccagctcg tcgtcgccgc actcgccatc cacggatcgg cggctgaagc gggtggcgtc
    21121 cacccaccgc agcaccggaa gatcccaggg acgcatcggc agctcggtga cgtcgctcag
    21181 cgagcgcacc acggcctgcg tcaggcccat gatggcggcc atcagcggca gcagcggcgc
    21241 cagcggcttg gccgccgtgc ggacggtacg gatgcgacgg gccaagacga gacgttcgat
    21301 cgagtgatac agccaggcgc ccacggccag cgcgtaaatc gacagcgtcg acgcgacgat
    21361 ggccagcccg aaggcggcgc atgcgacggc gcccagcacc acggtcgcgg ccagggcggc
    21421 ggccagcacg acacgtaact ccaaccgggt catggctgcg gtccccgatc gtcttgggcg
    21481 ccggaacgga ccgccgcagc ggccgcccga atctggggcg tcaccagaag tacctgaccc
    21541 agcaccccgt tgacgaagcc cggcgactcg tcggtggaca gctccttggc cagctgcacg
    21601 gcctcgtcga cggcgacggg ctccggcacg tcgtcggcat agagcagttc ccacaccgcg
    21661 acccgcagaa cggcccggtc caccgccggc agccggtcca gcgtccagcc caccagatgc
    21721 gagctgatca ggtcgtcgat gtgtgcggaa tgctcgctga cgccccgggc caccgccgcg
    21781 gtgtacgcgt tgagcggcgc cacatccgga ttcgtgccgg ccagcgcgcc gcgaacgtcg
    21841 acgacctccg ccgggctcag cccccgggcc tcggcttcga acaacaggtc gacggcacgc
    21901 ttgcgggcct gatgccgccc cttacccctc gccggtttgg ggtccggccc cgttttccgc
    21961 tccggcatga tcaggcgttt acccggccca ggtaactgcc gtcgcgggag tccaccttca
    22021 gcttgtcgcc ggtgttgatg aacaggggca cctggatctg cgcacccgtc tcgagggtgg
    22081 ccggcttggt gccggcgctg gaccggtcgc cctgcaggcc cggctcggtg tgggtgacct
    22141 ccaattcgac ggtcaccggc agctcgatgt acagcgccgc cccgttgtga aacgccacct
    22201 gcaccggcaa tccctccagc aggaaccgcg ccgcgtcgcc gaccagggtt tccggcagcg
    22261 gatgctgctc gtaatcctgg ctgtccatga acacgaagtc cgacccgtcg cggtacagat
    22321 aggtcgtgtc gcgccggtcg acggtggcgg tgtccacctt gacgccggcg ttgtacgtct
    22381 tgtccacgac cttgcccgaa agcacgttct tcagcttggt gcgcacaaac gccgggccct
    22441 tgccgggttt gacgtgctgg aactcggtga tggtccacag ctggccgtcg attaccagga
    22501 caagtccgtt cttgaagtca gcagtgctcg ccacggtcga agaatctcct acaaaatggc
    22561 cagttctttg gggaaccggg tcagcagttc cggggtctgc ccggcggtgt cacgagaggt
    22621 gagcgccccg ccggccacta ccaacgtgtc ctcgatgcgg acaccaccgc ggccgggcaa
    22681 atagacgccg ggctccacgg tcaccgcgga gcccgcaagt agtgtaccgg cggatgtcgc
    22741 cccaattccc ggtgcttcgt gaatctgcag gccaacgccg tgtcccaaac cgtggccgaa
    22801 ctggtcgccg tagcccgcat cgtcgatcag ccggcgcgcc gcgccgtcca cctcacgcag
    22861 gccggcgccc ggccgcaacg cctcccggcc ggcccgctgc gcctcggcga ccaactgata
    22921 gatctcgagc tgccagtcag cgggcttgcc cagcacgaac atgcgggtca tgtcggagtg
    22981 gtagccggcg accagcgcgc cgaagtcgat cttgacgaaa tcacccgcgg ccagcaccgc
    23041 gttggtcggg cggtggtgcg ggatcgccga atgcgcgccc gcggccacga tggtctcgaa
    23101 cgcgaccgcg tccgcgccgt ggtcgagcat cagggcctcc agctggcggc tgacctcacg
    23161 ttcggttcgg cccggccgca ggccgccgcg cgccaccagg tcggccagcg cggcgtcggc
    23221 cgcctcgcag gccaacctca gcagggccac ctcaccagcg tctttgacct cacgcaacgc
    23281 ctcgaccgtg ccggcggccc gcaccagctc ggtggcgccg ctgcgttcgc cgagctcgcc
    23341 ggtcagcgcg tcgtagccgt cgacggtgac cacattgctt tcgaaaccca gctttcccac
    23401 gtcggcctcg gcagcgcgcc ccaccaggta gcgaccgacg gcccgctcga tggcgatctc
    23461 gaggtcgggc gcctgcgcgg cggcctgggt gcggtaccgg ccgtcggtgg ccagcaccgg
    23521 gtcgcgatcg tcggcgaaca ccagcaacgc gccgttggac ccgctgaaac ccgatagata
    23581 tcgaacgttg accaggtccg tgaccagcat cgcgtccagc ccaccggcaa cgatttgcgc
    23641 tttcagattg tgtcgacgct gcgaatgtgt cacgaccctt gacggtactc gctacgctga
    23701 cttcctatga ggaactggat gctgcgcgga ttggtgttcg ccgcggtgat ggtcgtcgtc
    23761 cgcttgcttc aaggggcact gatcaatgcg tttcaggcgc aggccggcct gatcagtgcg
    23821 gtgctgctac tcctgtttgt gattgccgtt gccgcgtggg gggtggtcga cggccgggcc
    23881 gacgccgcgg ccaaccccga tccggatcgc cgccaggatc tggcgatgac ctggctgctg
    23941 gccggcatgc tggcgggggt cgtcagcggc gccgtgtcgt ggctgatctc gctgttctac
    24001 gacgggatat acaccggggg cgtgctcaat gagctcacga cgttcgcggc cttcaccgcg
    24061 ctcatggttt tcgtgcccgc gatcatcggc gtgagcatcg gccgctggcg ggtggaccgg
    24121 cagttcgcga agcagccgga gaagcaccac ggccttggcg ttaaagggga acgcgccgac
    24181 accgacgtgt tctccgccgt tcgcgccgac gacacgccga cgggcgagat tcccacccag
    24241 accaccggcg cccgccatga ggaacggacg gcggcggtgg cgacggccga gcgcgaggag
    24301 cccacccaga cgctcccgac gaccccgtgg ggcgcgccca ccaaagcaat gcccaccagc
    24361 gacgacgacg ccaacaccga ggtgatccgc acggacaggc acggcaagcc gggttcggaa
    24421 cccgagaagg gttagttctc agt
//
LOCUS       DASSGQ010000033        16916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_62715, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000033 DASSGQ010000000
VERSION     DASSGQ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 16916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16916
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62715"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFI55_01475"
     CDS             <1..190
                     /locus_tag="VFI55_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072738.1"
                     /translation="LVAEGVETPAAALTLMQHGCHRAQGFLLSRPVPGDAMEALLAAR
                     WMPMPFLADREALSMGAI"
     gene            202..1905
                     /locus_tag="VFI55_01480"
     CDS             202..1905
                     /locus_tag="VFI55_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019683569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072739.1"
                     /translation="MFGSLKIGKQAPVAGLAAVAVVASTALLLTGCGRQSNPGPLSAM
                     VSPAIPPTVQDVPNPLRGQYEDQLNPLFPQVNPAQQHYPAWPASSDASLSVSWRQLQP
                     TDPHTLPPDAPDDRKFDFSMIDDALTKLANRTMRLTLRVYAYKSCCNDSYPNNTNIAV
                     PDWMRSAATSYPGPPNGPTSGVTQVVPNWNDPTYLDGFTQLLGALGRRYDGDERLSVF
                     EFSGYGDSSENQISYLRDVPGAAGTAPEASAARLGYYSQYGDQSIAIAAIRQLVAANV
                     NAFPHTQLVASPHNPEIVRELFADDVTKRLSAPVGVRSDCLGVQSPLPVWAESSDSQY
                     VRTNDAVVNVIKQRLASALVISEWCRLPNGTDPRSYYEKGLHDVVRYHISMTSSANFP
                     DRDSTSVMDPALYLLWAQANASAGYRYSVEARPGSQSIQGAVATISVAWTNYGSAAVT
                     EQWAPGYKLVDFSGAVVRSLPATVNLKTTVSDDSSQSREEAVPATSDEAVHVDLTGLA
                     PGHYTLRASADWQQHKPGAAHVVNYAPMALARDGRDGSGLYPIATLDIPRDNPTSTNG
                     R"
     gene            complement(1946..2608)
                     /locus_tag="VFI55_01485"
     CDS             complement(1946..2608)
                     /locus_tag="VFI55_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET7072740.1"
                     /translation="MIPLSTGAIASIAAIGAHCNDIMVGAGATLLQIADRNPDVVVHA
                     LVLTGAGTAREVEEKNAFAALLPEVDVRLTVAELSEGNLPEEWGSVKRRLAEFRRRCQ
                     PDLVLGPHRDDRHQDRRLVAQLIPSEFRAHLVLGYEILKWESDLLKPTLYVEIPTRIA
                     QQKAKLLEQCYPSQSQRGWFDDEAFLGLMRVRGVQCRAQYAEAFAGEKAVVSPFGVHS
                     LN"
     gene            complement(2755..3045)
                     /locus_tag="VFI55_01490"
     CDS             complement(2755..3045)
                     /locus_tag="VFI55_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HET7072741.1"
                     /translation="MSFLTTEPAASAGELPRFGAPLDAGKASVAPPTTRSSLVAADDA
                     SARMAKQFAVHAAIYQAISAQALAILRQLVGVLTVGNGSQAPADAVKSHAAG"
     gene            3487..4164
                     /locus_tag="VFI55_01495"
     CDS             3487..4164
                     /locus_tag="VFI55_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7072742.1"
                     /translation="MTNNSADGRADLTRLQILRAAAHQFARRAYHDVGLDDILSDAEL
                     TKGAMYFHFRSKHALAVAIIESQTTAGTIALTDLLSRNLSGLETLIDFSYLIAAQDIH
                     AEGVRAGLNLLESVGRSEGLQEKLLDGWVQALAGVVEKAIADGDIDQGCSPRDVGRLI
                     VSLHMGLRQISNLDEPDRFLLDLERSWALVLTGILHPERFDYFSQFVRRRTALAIRGG
                     PTGLDSD"
     gene            4296..4997
                     /locus_tag="VFI55_01500"
     CDS             4296..4997
                     /locus_tag="VFI55_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7072743.1"
                     /translation="MARQVRSQATRRKILDAAIEVFSEVGYPAAAWGTIIERTGMTKG
                     ALYHHFDSKESLASAIIEEGSDTLLVAFRNVCGSASPALENMIHGTFTIANVLRSDKV
                     ARAAEQLTAALSGFNEAAARFCAGLVDLMAAEARRASAEGDLRPDLDAEVVSESIVGA
                     MVGIRLLSNAIPANGLSGDLISLTSQVWELLLPGLVTDDSLAYFRQFLGRETLRHARL
                     MESPETNTATVPEGT"
     gene            complement(5271..6830)
                     /gene="metG"
                     /locus_tag="VFI55_01505"
     CDS             complement(5271..6830)
                     /gene="metG"
                     /locus_tag="VFI55_01505"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET7072744.1"
                     /translation="MRPYYVTTAITYPNAAPHLGHAYEYVATDAVARFKRLDGFDVRF
                     LTGTDEHGLKVAQAAAAEGLPTAELARRNSDVFQRLQEKLNISFDRFIRTTDADHHEA
                     SKEIWRKMAAAGDIYLDSYQGWYSVRDERFFVESETAVVDGTRIAVETGTPVTWTEER
                     TFFFRLSDYTDKLLAHYEANPDFIAPEVRRNEVVSFVSGGLRDLSISRTSFDWGVQVP
                     EHPDHIMYVWVDALTNYLTGAGYPDTNSELFRRYWPADLHMIGKDIIRFHAVYWPAFL
                     MSAGIELPRRVFAHGFLLNRGEKMSKSVGNIVDPGTLIDTFGVDQVRYFLLREVPFGQ
                     DGSYSEDAIITRINTDLANELGNLAQRSLSMVAKNLGGVVPEPGELTADDTELLAAAD
                     GLLERVRANFDAQAMHLALEAIWLMLGEANKYFSAQQPWVLRKGESEADQARFRTVLY
                     ITCEVVRIAALLVQPVMPESAGKLLDLLGQAEYQRAFTAIETRLSPDTKLPAPTGVFP
                     RYQPPVSTEVE"
     gene            6849..7712
                     /locus_tag="VFI55_01510"
     CDS             6849..7712
                     /locus_tag="VFI55_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET7072745.1"
                     /translation="MAVTSSRPGKPEAPPAPEPLAPLIDAHTHLDACGDTRVRGAEGV
                     RAIVERAAAVGVGAVVTVADDLDSARWVTRAAEWDPRVYAAVALHPTRANALNDAARA
                     EIERLAGHPRVVAVGETGMDLYWPGRLDGCAQPDVQREAFAWHIDLAKRSGKPLMIHN
                     READAAVLDVLAGEGAPDVVIFHCFSSDSAMARRCVDAGWFLSLSGTATFRNARALRE
                     AVPLIPPAQLLVETDAPFLTPHPYRGAANEPYCLPYTLRAIAELVEHDPGELAQVTTS
                     NARRVYGLASE"
     gene            7878..9005
                     /locus_tag="VFI55_01515"
     CDS             7878..9005
                     /locus_tag="VFI55_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HET7072746.1"
                     /translation="MNVLTKLHQNPSPMLRLVVAGLLLVLGLAGGFAVSASKTVTLTV
                     DGIAMRVTTMKSRVVDIVQENGFAVGERDDLYPAGDVEVRDAANIVLRRSRPLQISLD
                     GRDAKQVWTTASTVDEALAQLAMTDTAPAAASRGSRVPLAGMALPVVSAKTVQINDGG
                     AVRTVHLPAPTVAGLLSAAGAPLLDSDQVVPSATSPIADGMEIQVTRNRIQHVTERIP
                     LPPNSRRIEDPEMNMSRQVVEDAGSPGTQDVTFAVAMVNGVETGRIPVANTVIAPARE
                     AVLRVGIKPGTDVPPVTNGSIWDAISGCESGGNWAINTGNGFYGGVQFDFGTWIANGG
                     LRFAPRADLATREEQIAIAEVTRARQGWGAWPVCSGRAGAS"
     gene            8969..9931
                     /gene="rsmA"
                     /locus_tag="VFI55_01520"
     CDS             8969..9931
                     /gene="rsmA"
                     /locus_tag="VFI55_01520"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015289494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET7072747.1"
                     /translation="MARVQRKSWCKLTIQLLGRTEIRRLAKDLEFRPRKALGQNFVHD
                     ANTVRRVVSTSGVSRSDRVLEVGPGLGSLTLALLDRGAAVIAVEIDPVLAGRLPQTVA
                     EHSNSEIHRLTVVNHDVLTLRRDELATEPTALVANLPYNVAVPALLHLLAEFPSIQTV
                     TVMVQAEVAERLAAEPGGKEYGVPSVKARFFGRVRRCGTVSPTVFWPIPRVYSGLVRI
                     DRYATSPWPTDEVFRRQVFQLVDIAFAQRRKTSRNAFAEWAGSGNESASRLLAASIDP
                     ARRGETLSIDDFVRLLQRSGNRDDAHPARPDAAQPHISGHVSAG"
     gene            9995..10975
                     /locus_tag="VFI55_01525"
     CDS             9995..10975
                     /locus_tag="VFI55_01525"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HET7072748.1"
                     /translation="MTMSDGNTAAQWVPTGSVTVRVPGKVNLYLAVGDRRKDGYHELT
                     TVFQAVSLLDEVTVRNADVLSLELVGEGADKLPTDERNLAWQAAELMADHVGRAPDVS
                     IMIDKSIPVAGGMAGGSADAAAVLVAMNSLWELNVPRRDLRTLAARLGSDVPFALHGG
                     TALGTGRGEELATVLSRNNFHWVLALADGELLTSAVYAKLDRLREKRSPPRLAEPGPV
                     LAALAAGDPEQLAPLLGNELEAAAVSLNPALRRALRAGVQVGALAGIVSGSGPTCAFL
                     CASAASAVDVATELSAAGVCRTVRVASGPVPGARVVPAPTTPHDQGLNGV"
     gene            11232..12866
                     /locus_tag="VFI55_01530"
     CDS             11232..12866
                     /locus_tag="VFI55_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HET7072749.1"
                     /translation="MSRFTDKMYLNARTSTTGMVTGEPHEPIRHTWGEVHERARCIAG
                     GLAAAGIGLGDAVGVLAGFPVEIAPTAQGLWMRGASLTMLHQPTPRTDLAVWAEDTMT
                     VIGMIEASAVIVSDPFVAAIPVLEDKGITVLTIADLLASEPIDPVEVGEDDLALMQLT
                     SGSTGSPKAVQITHRNIHSNAEAMFVGAEYNVDRDVMVSWLPCFHDMGMVGFLTIPMY
                     FGAELIKVTPMDFLRDTLLWAKLIDKYQGTMTAAPNFAYALLAKRLRRQAKPGQFDLS
                     TLRFALSGAEPVEPADVEDLLDAGKPFGLRPSAILPAYGMAETTLAVSFSECNAGLVV
                     DEVDADLLAALRRAVPASKGNTRRLATLGPLLNDLEARIIDEHGDVMAARGVGVIELR
                     GESLTPGYLTMGGFIPAQDEHGWYDTGDLGYLTEEGHIVVCGRVKDVIIMAGRNIYPT
                     DIERAACRVEGVRPGCAVAVRLDAGHSRETFAVAVESNAWQDPAAVRRIEHQVAHEVV
                     AEVDMRPRNVVVLGPGTIPKTPSGKLRRSTSVSLVS"
     gene            complement(12887..13462)
                     /gene="pth"
                     /locus_tag="VFI55_01535"
     CDS             complement(12887..13462)
                     /gene="pth"
                     /locus_tag="VFI55_01535"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET7072750.1"
                     /translation="MAEPQLVVGLGNPGDNYARTRHNLGFMVADVLAARLGAKFKAHK
                     RSGAEVVSGRLAGHPVLVAKPRCFMNESGRQVGPLAKFYSVAPADIIVIHDDLDLEFG
                     CIRLKIGGGEGGHNGLRSVAAALATKDFQRVRIGIGRPPGRKDPAAFVLENFTAAERT
                     EVPTICEQAADATELLIELGLEPAQNRVHAW"
     gene            complement(13475..14143)
                     /locus_tag="VFI55_01540"
     CDS             complement(13475..14143)
                     /locus_tag="VFI55_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HET7072751.1"
                     /translation="MAKGTVNQLAVTVRTETGKGASRRARRAGNIPAVLYGHGADPQH
                     LELPGHDFAAVLRHSGTNAVLTLNIGGKEQLALTKALDIHPIRRTIQHADLLVVRRGE
                     KVVVEVTVVVEGEAASDTLVTQETTTIEIEAEALSIPEQLTVSVQDAEPGTQFTAGQV
                     ALPRGINLISDPELLVVNVVNAPTAEDLEEEGAGEVAEGEETPEEAAEAEEAGEAAAA
                     EESE"
     gene            complement(14414..15283)
                     /locus_tag="VFI55_01545"
     CDS             complement(14414..15283)
                     /locus_tag="VFI55_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET7072752.1"
                     /translation="MTDWTAADLPSFAGRTAIITGANSGLGAVTARELARNGARVILA
                     VRNTAKGEAAAQQMTGTIEVHRLDLQDLSSVRQFADGVDGVDILVNNAGIMAAPYALT
                     VDGFESQIGTNHLGHFALTNLLLPKLTDRVVTVSSMAHWPGRIDFDDLNWRSRRYSPW
                     AAYGQSKLANLLFTSELQRRLDSVGSPLRALAAHPGYSHTNLQGASGRRVSDALISAA
                     TRVVATDADFGARQTLYAASQDLPGDTFVGPRFGYIGPTQPVGRSRRARDAAAATSLW
                     ELSEQLTGTKFPL"
     gene            complement(15314..15946)
                     /locus_tag="VFI55_01550"
     CDS             complement(15314..15946)
                     /locus_tag="VFI55_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqN/LpqT family lipoprotein"
                     /protein_id="HET7072753.1"
                     /translation="MAAAVVVLVAGACAPKAPDYQSILSKSSTAAATTTTTTDKPVPL
                     SQYLEDIGVIGKQVAPGSLTDLAVSIPTPPGWSPFTNPKITPETLIIAKDRKYPTARL
                     VVFALRGDFDPAKVIKHGNDDAQLFENFKQLDASTADYNGFPSSMIQGSYDLDGTRLH
                     SWNRIVIPTGSPPANQRYLVQLTITSLADQAVAQSSDIEAIIRGFVVAAK"
     gene            complement(15958..16326)
                     /gene="arsC"
                     /locus_tag="VFI55_01555"
     CDS             complement(15958..16326)
                     /gene="arsC"
                     /locus_tag="VFI55_01555"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254208.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HET7072754.1"
                     /translation="MAPVTGETSHNTVIYHNPKCSTSRKTLDLLRDNGVEPTIVQYLK
                     TPPSRAELVRMIHDAGIDVRTAVRKREALYTELNLADVTDDELLDAMAEHPILIERPF
                     VVTPKGTRLARPIDTVREIL"
     gene            complement(16327..>16916)
                     /gene="prsA"
                     /locus_tag="VFI55_01560"
     CDS             complement(16327..>16916)
                     /gene="prsA"
                     /locus_tag="VFI55_01560"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET7072755.1"
                     /translation="IVTVDLHTDQIQGFFDGPVDHMRGQNLLTGYIKDNYPDGNMVVV
                     SPDSGRVRIAEKWADSLGGVPLAFIHKTRDPRVPNQVVSNRVVGEVAGRTCVLIDDMI
                     DTGGTIAGAVKLLREAGASDVVIAATHGVLSDPAAQRLAACGAREVIVTNTLPIGEEK
                     RFPQLTVLSIAPLLASTIRAVFENGSVTGLFDGDA"
BASE COUNT         2799 a         5477 c         5649 g         2991 t
ORIGIN      
        1 actggtcgcc gagggagtcg agacacccgc tgccgcactg actttgatgc aacacggctg
       61 ccaccgggcg cagggattct tattgtccag accggtcccg ggcgacgcca tggaagcttt
      121 gctggccgcg cgctggatgc ccatgccgtt cctcgccgac cgcgaggcgc tctcgatggg
      181 cgcaatctag catcggtggc gatgtttggt tcgttgaaaa tcggcaaaca ggcccctgtg
      241 gctggtctgg ctgcggtagc cgtcgtggcg agcacggcac tgctgttgac cggatgtggt
      301 cgtcaaagta acccgggacc attgtcggcg atggtcagtc cagccatacc gcccacggtt
      361 caggatgtac cgaatccgtt gcgcggccag tacgaggatc agctgaatcc gcttttcccg
      421 caagtcaatc ccgcacagca gcactatccg gcgtggcccg cgtcatccga cgccagtttg
      481 agtgtctcgt ggcggcagct gcagcccacc gatccgcaca cgctgccccc ggacgcaccc
      541 gacgaccgca agttcgactt cagcatgatc gacgacgctc tcacgaagct ggccaaccgg
      601 accatgcggc tgactcttcg tgtgtatgcc tacaagtcgt gttgcaacga ttcctacccg
      661 aacaacacga acatcgcggt ccccgattgg atgcgctcgg ctgccaccag ctatcccggg
      721 ccgccgaacg gccccacgtc aggggtgacg caggtggtgc cgaactggaa cgatccgact
      781 tatctggacg gcttcaccca gctgctcggc gcgctgggcc gccgctacga cggcgacgag
      841 cgcttgagcg ttttcgagtt ctcgggttac ggggattcga gcgagaacca gatctcgtat
      901 ttgcgcgacg tgccgggcgc ggcgggtacg gcacccgaag ccagcgccgc caggctcggg
      961 tactacagcc agtacggcga tcagagcatt gccatcgcag ccatccggca gttggtcgcg
     1021 gcgaacgtca atgcgtttcc gcatacgcaa ttggtggcca gcccgcacaa cccggagatc
     1081 gtgcgcgagc tctttgctga cgacgtcaca aagcggctct ccgcgccggt gggcgtccga
     1141 tcggattgcc ttggtgtgca atcaccttta ccggtgtggg ccgagtccag cgactcccaa
     1201 tatgtgcgga ccaacgacgc ggtcgtcaat gtgatcaaac agcggctcgc ctcggctctg
     1261 gtgatcagcg aatggtgccg gctgcccaat gggaccgatc cgcggtccta ctacgaaaag
     1321 ggtctgcacg acgtcgtgcg gtatcacatt tcgatgacgt cgagcgccaa cttcccggac
     1381 cgggattcga cgtcggtgat ggatcccgcg ctgtatctcc tctgggcgca ggccaacgcc
     1441 tcggccggat accggtactc ggtcgaggcg cgaccgggat cacagtcgat acagggcgcg
     1501 gtggcgacca tctctgtcgc ctggacgaac tacggctcgg ccgccgtcac cgagcagtgg
     1561 gcacccggct ataagctggt ggacttttcg ggggcggtgg ttcgcagcct cccggcaacg
     1621 gtcaacctca agacgacggt gagcgacgac tccagccaat cacgcgagga agcggtcccg
     1681 gcgacgtccg acgaggcggt tcacgtcgac ctgacgggtt tggcgcccgg ccactacacg
     1741 ctgcgcgcgt ccgcggattg gcagcagcac aagccgggcg ccgcgcacgt ggtgaattac
     1801 gcacccatgg cgttggcccg tgacggccgc gacggctcgg ggttgtaccc gattgccacg
     1861 cttgacatcc cacgcgacaa cccgacgtcg accaacgggc gatgaggcgc cggcgcccgt
     1921 ccgggccgca ggcatcatcg cgcacttaat tgagcgagtg aacgccgaag ggactcacga
     1981 cggccttctc cccggcaaag gcttcggcgt actgtgcccg gcactgcacg ccgcggactc
     2041 gcatcaagcc gaggaacgct tcatcatcga accatccgcg ctgcgattga gacggatagc
     2101 actgctcgag cagcttcgcc ttctgttggg cgattcgcgt cgggatctct acgtagaggg
     2161 ttggcttgag caggtccgat tcccacttga gaatctcgta gcccagcacc aggtgcgcgc
     2221 ggaactcgct cgggatcagc tgcgcgacca agcggcgatc ctggtgacgg tcgtcacggt
     2281 gtggaccgag caccagatcc ggctggcagc ggcggcgaaa ctccgcgagg cggcgcttta
     2341 ccgatcccca ctcttccgga aggttgcctt cagatagctc ggctaccgtc agccggacgt
     2401 ccacctccgg cagtagcgcc gcgaaggcgt tcttctcctc gacctcgcgc gcggtgccgg
     2461 caccggtgag caccaacgcg tgcacgacaa catcgggatt gcgatcggcg atctgcagca
     2521 acgtcgcgcc ggcgccgacc atgatgtcgt tgcagtgcgc cccgatggcg gcgatcgagg
     2581 cgatggcgcc ggtgctaagc gggatcatgc cgaacgtctc gtcgctcgag gtggctcatc
     2641 gtcccatgtc agagccgctt tctgtggcga ccccgagccc gcagcggcat acattctcgt
     2701 ggtatcgaat gtcggtggga acgcgacgat gctcatcgct tcgcatccag ctcgttagcc
     2761 ggccgcgtgc gatttgacgg cgtcggccgg ggcttgcgaa ccattgccga ccgtcaagac
     2821 gccaacgagc tgccggagaa tcgccagggc ttgggcgctg attgcctggt atatcgcggc
     2881 atgtacggcg aattgcttcg ccatccgcgc cgacgcgtcg tcggccgcca cgagggacga
     2941 tcgggtcgta ggaggtgcca cgctcgcctt tccggcgtcc agcggcgcgc cgaaccttgg
     3001 tagctcgccg gccgatgccg ccggctcagt agtcaggaac gacatgtgct tctcctaacg
     3061 ggtgcagatc cgaccgggat cgggtgtggc ggctgagcga agcgtccaag cgttgctgga
     3121 aaccgaacgc ggaaagcaaa ccaacagcca catgacaagg gagtatacat actgttagta
     3181 tgtcaacaac catggaactg gctaccggta tttgttttcg ttatgtttgg tgatcaacgt
     3241 tgagaatctg cgagctacta catactgcta acctcagggc cggccgacat gccgtccgag
     3301 gtccgcttat gagtgctcgc cccggccgca gtgtgcggct tgctcgaggg agggcatcgt
     3361 gaacgtaacg gcgatcgacc cgccccggca cgcgggcctg gacagccgtg tcccgcgtgg
     3421 ccatcgtcgc gctcgccaac gcggaccaca atcacccggt acccctcacg tcagcccgtt
     3481 gttgcgatga cgaacaactc ggctgacgga cgcgcagact tgacgcggct ccagattctg
     3541 cgagccgccg cacaccagtt cgctcgacgg gcctaccacg acgtgggcct cgacgacatc
     3601 ctgagcgacg ccgaattgac caagggcgca atgtatttcc acttccgttc caagcacgcg
     3661 ctggcggttg cgatcatcga gagccagacc accgcgggta ccatcgccct gacggacctg
     3721 ctttctcgaa acctttcggg cctcgaaacg ctgatcgact tctcctattt gatagctgcg
     3781 caggacatcc acgcggaggg cgtcagagcg ggactgaact tgctcgaatc ggtcgggcgg
     3841 tcggaggggc tgcaggagaa actgttagac ggatgggtgc aagcgctcgc cggggtcgtt
     3901 gagaaagcca ttgccgacgg tgacatcgac cagggttgca gcccacggga tgtcggacgc
     3961 ctcatcgtgt cgctccatat ggggttgcga cagatcagca acctcgacga gccggaccgg
     4021 ttcctcctcg acctggagcg cagctgggca ctggtgctga cgggaatcct gcatccggaa
     4081 cgattcgatt acttcagcca attcgtcagg cgacgcaccg cactcgcgat ccgcggcggt
     4141 ccgaccggcc tggattccga ttgaaaaacc ccgcgggccg gaggatcggg ccgattgcgg
     4201 gttgatgttg ttaggcttgg tcctttgccg gcaaaagacg agaagcccag atccggaatc
     4261 tgaaaagatc acggaactcc ggaggtgcag gagcgatggc gcgccaggtg cgatcccaag
     4321 ccacccgtcg gaaaattctg gacgcggcga ttgaagtctt cagcgaggtc gggtatccgg
     4381 ccgctgcgtg ggggacgatc atcgagcgga cggggatgac caagggggcc ctgtatcacc
     4441 atttcgactc caaggaatcg ctggcgtccg ccatcatcga ggagggttcg gacaccctcc
     4501 tggtcgcctt ccgcaatgtt tgtgggtcgg cctcaccagc gctggagaac atgatccacg
     4561 gcacgttcac gatcgcgaat gtgctgagat ccgacaaggt ggcccgcgcg gctgagcaat
     4621 tgaccgctgc gctgagcgga ttcaacgaag cggcggcgcg attctgcgca ggcttggtgg
     4681 atttgatggc cgccgaggcc cggcgggcaa gcgccgaggg agacctccgg ccagacctcg
     4741 acgcggaggt ggtcagcgag tcgatcgtgg gcgcaatggt aggaattcga ttgctgtcca
     4801 acgcgattcc cgctaatggt ctcagtggag acctgatcag tctaacgagt caggtgtggg
     4861 aactgctgct tcccgggctc gtcaccgacg actcattggc gtatttccgg cagttccttg
     4921 gacgcgagac tctccggcac gcccggctaa tggagtctcc cgagacgaac accgccacgg
     4981 tgccggaagg tacatagccc gtcgcgacgt cgcgggtttg gttgcgtgcc aatcgtttcg
     5041 acgaccggcg cgacggaacg cgggttgacc cgggggatga gtgagcaacc cgccggtcgt
     5101 cgatggtgat gcgcaagcgg ctacccgcgc atcatcggtt acgcaattcg attgcggctc
     5161 aacaagttcc cgtcgctgtg ctgggttgct cgtctcactc gcatcacttc gttctgctcg
     5221 ttcgacttcg gtgctggatt gctcgtctca ctcgcgtcac ttcgttccgc tcactcgact
     5281 tcggtggaaa cgggcggttg gtagcgcggg aacaccccgg tgggcgccgg caacttcgtg
     5341 tcgggggaga gccgcgtctc gatggcggtg aaagcccgtt ggtactcggc ctggccgagc
     5401 aagtccagca gcttgccggc cgactcgggc atgacgggct gaaccagcag cgccgcgatc
     5461 cggacaactt cgcaggtgat gtacaggacc gtacgaaacc tcgcttgatc ggcctccgac
     5521 tcgcccttgc gcagcaccca cggctgctgc gcggagaagt acttgttcgc ttccccgagc
     5581 atcagccaga tcgcctcgag cgccaggtgc atcgcctgcg cgtcgaagtt ggcccgtacc
     5641 cgctccagca atccgtcggc ggccgccagc agctcggtgt cgtcggcagt cagctcgccg
     5701 ggctcgggca ccaccccgcc caggttcttg gccaccatcg acaacgatcg ctgagccaga
     5761 ttgcccagct cgttggccag gtcggtgttg atccgggtga tgatcgcgtc ttcgctgtag
     5821 ctgccgtcct ggccgaacgg gacctcgcgc agcaggaagt agcgcacctg atcgacgccg
     5881 aacgtgtcga tgagtgtgcc cgggtcgacg atgttgccca ccgacttgct catcttctcg
     5941 ccacggttga gcagaaaccc gtgcgcgaaa acccttcgcg gcagctcgat tccggccgac
     6001 atcaaaaacg ccggccagta cacggcgtgg aaacggatga tgtccttgcc gatcatgtgc
     6061 aagtcggcgg gccagtagcg gcggaacaac tccgaattgg tgtcagggta gcccgccccg
     6121 gtcaggtagt tggtcagcgc gtcgacccag acgtacatga tgtgatcggg atgctcgggc
     6181 acctgcacgc cccagtcgaa cgaggtccgc gagatcgata ggtcacgcag cccgccggag
     6241 acgaaactga ccacttcgtt gcgccgcacc tcgggtgcga tgaagtccgg gttggcctcg
     6301 tagtgggcca gcagcttgtc ggtgtagtcc gacagccgga agaaaaacgt ccgctcctcg
     6361 gtccaggtca ccggggtgcc ggtttcgacg gcgatccggg tgccgtccac caccgcggtc
     6421 tccgattcga cgaagaatcg ctcgtcgcgc accgaatacc agccctgata gctgtccagg
     6481 tagatatcgc ctgcggccgc catcttccgc cagatctcct tggacgcttc gtggtggtcc
     6541 gcgtcggtgg tgcggatgaa gcggtcgaag gagatgttca acttctcctg caggcgctgg
     6601 aacacgtcgg aattgcgacg ggccagttcg gcggtgggta gcccttcggc cgcggcggcc
     6661 tgggcaacct tgagcccgtg ctcatcggtt ccggtcagga agcgcacgtc gaaaccgtcg
     6721 aggcgcttga accgtgcgac ggcgtcggtg gcgacgtact cgtacgcgtg ccccaagtgc
     6781 ggcgcggcat tggggtaggt gatcgcggtg gtgacgtaat agggcctcat cgagaatcac
     6841 cctattgtgt ggccgtgacc tccagccgcc ccggtaaacc agaagcgccg cccgcgcccg
     6901 agccgttggc gcccctgatc gacgcccaca cccacctcga cgcgtgtggc gacactcggg
     6961 tcaggggagc cgagggagtg cgggccatcg ttgaacgcgc cgcggcggtc ggggtgggag
     7021 cggttgtcac cgtcgccgat gacttggact cggcgcgctg ggtgaccagg gccgccgagt
     7081 gggatccgcg ggtttatgcc gcggtggcgt tgcaccccac ccgcgcgaac gcgctgaacg
     7141 acgccgcccg tgccgagatc gagagactcg cgggacaccc cagggtggtg gccgtcggcg
     7201 agaccgggat ggatctctac tggcctgggc ggctggacgg ctgcgcgcag ccggacgtcc
     7261 agcgggaagc cttcgcgtgg catatcgacc tggccaagcg gagcggcaag ccgttgatga
     7321 tccacaaccg agaggccgac gccgcggtac tcgacgtcct ggcgggcgag ggcgccccgg
     7381 acgtggtgat cttccactgc ttttcgtcgg acagcgcgat ggcccgccgg tgcgtggacg
     7441 cgggctggtt cctcagcctt tccgggaccg cgaccttccg caacgctcgc gccctgcgcg
     7501 aggccgttcc gctgataccg ccggcacagc tgctggtgga aaccgatgcg ccctttttga
     7561 cgccgcatcc ctatcggggc gcagcgaacg agccgtattg ccttccctac acgctgcgcg
     7621 cgatcgctga actggtagag cacgatcccg gagagctggc gcaggtcacc accagcaatg
     7681 cccgccgggt ctatggactg gcttctgagt agcgttttcg ccagccgaat cccttaacac
     7741 catcgctccg gagttgctca agattggccg gttcgttacc gtcttgtgat cgaacaccgg
     7801 ggcccttggg cccatttgtc attgtatgtg tgctgattag gtcgttaagt agttgctgag
     7861 gtcggggtag acgcgctttg aatgtattga caaaacttca tcagaaccca tcgccgatgt
     7921 tgcggctcgt ggtcgcaggg ttgctgttgg tactcggttt agccggcgga tttgcggttt
     7981 ccgcatccaa gacggtgacg ctgaccgtcg atggaatcgc gatgcgggtg acgaccatga
     8041 agtcgcgggt ggtcgacatc gtccaggaga acggcttcgc cgtcggcgag cgcgacgacc
     8101 tctaccccgc gggtgacgtg gaggtccgcg acgcggccaa catcgtcttg cggcgtagcc
     8161 ggccgttgca gatctcgctg gacggtcgcg acgccaagca ggtctggacg acggcatcca
     8221 ccgtggacga ggcgctggcc cagctcgcga tgaccgacac cgccccggcc gcggcctcgc
     8281 gcggcagccg tgttccgctg gccgggatgg cgctgccggt cgtcagcgcc aagacggtcc
     8341 agatcaacga cggcggcgcg gtgcgcaccg tgcacctgcc ggcacccacc gtcgcggggc
     8401 tgctcagcgc cgccggcgct ccgctgctcg acagcgacca ggtggtgccc agcgcaacat
     8461 ccccgatcgc tgacggcatg gaaatccagg tgacccgtaa ccggattcaa catgtcaccg
     8521 agcgaattcc gctgccgccg aactcgcgcc ggatcgagga cccggagatg aacatgagcc
     8581 gccaggtcgt cgaggacgca ggcagcccgg gcacgcagga cgtgacgttc gcggtggcca
     8641 tggtcaacgg cgtcgagact ggccggatac cggtcgccaa caccgtcatc gcgccggccc
     8701 gtgaagccgt gctgcgggtg ggcatcaagc ccggcaccga tgtgcccccg gtgaccaacg
     8761 gatcaatctg ggacgccatc tcgggctgcg aatccggcgg aaattgggca atcaacacgg
     8821 gcaacgggtt ctacggcggc gtgcaattcg actttggtac gtggatagcc aacggcgggc
     8881 tgcggtttgc gccccgcgcc gacctcgcca cccgcgaaga gcagatcgcc atcgcagagg
     8941 tgacccgggc gcgtcagggt tggggggcgt ggcccgtgtg cagcggaaga gctggtgcaa
     9001 gctgaccatc cagcttctcg ggcgcaccga gatcaggcgc ctggccaaag atcttgaatt
     9061 tcggccacga aaagctttgg gccaaaattt cgtccacgac gccaacacgg tgcgacgggt
     9121 ggtgtccacg tccggggtca gccgctccga ccgcgtcctg gaggtcggtc ccggcctcgg
     9181 gtccctgacg ctggcgctgc tcgaccgtgg cgccgccgtc atcgccgtcg aaatcgaccc
     9241 ggtgctggcc ggtcgcctcc cgcagaccgt cgccgagcat tcgaatagtg aaatccaccg
     9301 gctgacggtg gtgaaccacg acgtgttgac gcttcgccgt gacgagctgg ccactgagcc
     9361 gacagcccta gtcgccaacc tgccctacaa cgtcgcggtc ccggcgctgt tgcatctgct
     9421 cgccgaattt ccgtcgattc agaccgtgac ggtgatggtg caggccgagg tcgccgaacg
     9481 gctggccgcc gagccgggcg gcaaggaata cggcgtgccc agcgtcaagg cccgtttctt
     9541 cgggcgggtt cgtcgttgcg gcacggtctc gccgaccgtc ttctggccga tcccgcgtgt
     9601 ctattccggg ctggtgcgca tcgaccggta cgcgacttcg ccgtggccga cagacgaggt
     9661 ctttcggcgg caagtgttcc aactcgtcga catcgcgttc gcccagcggc gcaagacgtc
     9721 ccgcaatgcg ttcgccgagt gggccggctc gggcaacgag tcggcgagcc gattgctggc
     9781 ggccagcatc gatcctgcgc gtcgcggcga gacgttgtcc atcgatgact tcgtgcggct
     9841 gttgcagcgt tccggcaatc gggacgacgc ccacccagca cgccctgacg cggcgcagcc
     9901 gcacatttcg ggccacgttt ccgcgggctg acttggtgct ctggagcgcc gttgcgtgtg
     9961 cttcaccatt ggcagcgata gtctgctgcg gtggttgacg atgtctgacg gcaacaccgc
    10021 tgcccagtgg gtgccaaccg gctcggtcac cgttcgggtg cccgggaagg tcaacctcta
    10081 cctggcggtc ggtgaccgac gcaaagacgg ctaccacgaa ctgacgacgg tgtttcaggc
    10141 cgtttcgctg ctcgacgagg tgacggtccg caacgccgac gtgctgtcgc tcgagctcgt
    10201 cggcgagggc gccgacaaac tgcccaccga cgaacgcaat ctcgcctggc aggcggccga
    10261 gctgatggcc gaccacgtcg gccgggctcc cgatgtctcg atcatgatcg acaaatccat
    10321 tccggtggcc ggcggcatgg ccggcggcag tgcggacgcg gcggcggtgc tggtcgcgat
    10381 gaactcgctg tgggaactca atgttcctcg ccgcgacctg cgcacgcttg ccgcgcgact
    10441 gggcagcgat gtgcccttcg cgctccacgg cggcaccgcg ttgggaaccg gtcgcggcga
    10501 ggagctggcc accgtcctgt cccgcaacaa cttccactgg gtgctggcgt tggccgacgg
    10561 cgagctgctc acttcggcgg tgtacgccaa gctggaccgg ctcagggaga aacgcagccc
    10621 gccgcgactt gccgagccgg gcccggtgct ggccgccctg gccgcgggcg atccggagca
    10681 gcttgcgccg ctgctgggca acgagctgga ggcggccgcg gtgagcctga acccggcgct
    10741 gagacgtgcg ctgcgcgcgg gggtccaggt gggtgccctg gcgggcatcg tgtcgggttc
    10801 tgggccgacc tgcgctttcc tgtgcgcctc agcggcctcg gcggtcgatg tcgcgaccga
    10861 gctgtcggcg gcgggcgtgt gccgcaccgt ccgggtggcc agcggaccgg tgccgggcgc
    10921 ccgcgtggtg ccggcgccca cgacccccca tgatcagggc ctgaacggag tgtgaaatcg
    10981 aactcaaatc gcgccttggt ttggcagttc cttaagagga gtttaagata attcgcgatg
    11041 gggagcagcg ataagcaagg cctctcagct tcgtcgtccg ccgggcctac cggcggactc
    11101 gttgtcgcat catcgattcg agacctaacc gcggcaaaac tgtgctcgcg tgcctgctac
    11161 aaagtggcaa cccgcaggcg gaatataaga tcccgggcct tcgggaatgc gccggaaccg
    11221 aggaggtttt tgtgagcagg tttaccgaca agatgtacct caacgcccgg acgtcgacga
    11281 cgggcatggt caccggtgaa ccccacgaac ctatccgtca cacctggggt gaggtccacg
    11341 agcgcgcccg ttgcattgcg ggcggcctgg cggcggccgg catcggcctc ggcgacgcgg
    11401 tcggtgtgct ggccggcttc ccggtggaga tcgcgccgac ggcgcagggc ctatggatgc
    11461 gcggggccag cctcaccatg ctgcaccagc ccaccccacg caccgacctg gccgtgtggg
    11521 ccgaggacac catgaccgtc atcggcatga tcgaggccag tgccgtcatc gtctcggacc
    11581 ccttcgtggc ggccatcccg gtgctcgagg ataagggcat cacggtcctt accatcgccg
    11641 acctgctggc gtcggaaccg atcgaccccg tggaggtcgg cgaggacgac ctggcgctga
    11701 tgcagctgac atccggatcc acgggttctc cgaaagccgt gcagatcacc caccgcaaca
    11761 tccactccaa cgccgaggcg atgttcgtgg gcgccgagta caacgtcgac agggacgtca
    11821 tggtcagctg gctgccgtgc ttccacgaca tgggcatggt cggcttcctc accattccga
    11881 tgtacttcgg cgcggagctg atcaaggtca cgccgatgga cttcctgcgc gacacgttgc
    11941 tgtgggccaa gctcatcgac aagtaccagg gcaccatgac cgcggcaccc aacttcgcct
    12001 acgcgctgct ggccaagcgg ttgcgccgcc aggccaagcc cgggcagttc gacctgtcga
    12061 cgctgcgatt cgccctgtcc ggcgccgagc ccgtcgaacc cgccgacgtc gaggacctcc
    12121 tcgacgcggg caagccgttc ggcctgaggc cctcggcgat cctgccggcc tacggcatgg
    12181 ccgagacgac gctggccgtg tcattctcgg agtgcaacgc cggtctggtc gtcgacgagg
    12241 tcgacgccga cctgctggcc gccctgcgcc gggccgtacc cgccagcaag ggcaacaccc
    12301 gccggctggc cacgctgggt ccgctgctca acgatctcga ggcccgcatc atcgacgagc
    12361 acggcgacgt gatggccgcg cgcggtgtcg gtgtgatcga gctgcgcggc gagtcgctga
    12421 cgcccggcta cctcaccatg ggcggcttca ttccggccca ggacgagcac ggctggtacg
    12481 acaccgggga cctcggctat ctcaccgagg agggccatat cgtggtgtgc ggccgcgtca
    12541 aggacgtcat catcatggcg ggccgcaaca tctatccgac cgacatcgag cgggccgcct
    12601 gccgggtgga gggcgttcgg cccggttgcg ccgtcgcggt gcgcctcgat gccgggcact
    12661 cacgcgagac gttcgccgtg gcggtggagt ccaacgcctg gcaggacccg gccgcagtgc
    12721 gccgcatcga acaccaggtc gcccacgaag tggtggccga ggtcgacatg cggccgcgca
    12781 atgtcgtggt gctgggcccg ggcaccatcc cgaagacgcc gtcgggcaag ctgcgccgct
    12841 ccacctcggt cagcctcgtc agctagaggc caaagcttct cgaggcctac caggcgtgga
    12901 cgcgattttg cgcgggttct aaccccagtt cgatgagcag ctcggtggcg tcagccgcct
    12961 gctcgcagat cgtcggcacc tcggtgcgct cggcggcggt gaagttctcc aacacgaacg
    13021 ccgccgggtc tttgcgtccc ggcggacggc cgatcccgat gcgcacccgc tggaagtctt
    13081 tggtggccag cgcggccgcc accgagcgca gcccgttgtg gccaccctca cctccgccga
    13141 tcttgagccg gatgcagccg aactcgaggt cgaggtcgtc gtggatgacg atgatgtcgg
    13201 ctggggcgac cgagtagaac ttcgccagcg gccccacctg gcggccggat tcgttcatga
    13261 agcagcgcgg cttggccacc aacaccgggt gcccggccag tcggccgctg acaacttcgg
    13321 caccggatcg cttatgcgcc ttgaacttcg cacccagccg ggcggcgagc acgtcggcaa
    13381 ccatgaaccc gaggttgtgc cgggtgcggg cgtagttgtc tccggggttg cccaggccga
    13441 ccaccaattg gggttcggcc acgtcgtgaa ccctctactc ggactcttcg gcggccgcgg
    13501 cttcgccggc ctcttctgct tctgccgctt cttccggggt ttcctcgccc tcggcgacct
    13561 cgccggcgcc ctcttcctcg aggtcctcgg ccgtcggcgc gttcaccacg ttgaccacca
    13621 gcaattcggg gtcggaaatc aggttgatgc cccggggcag cgcgacctgt ccggcggtga
    13681 actgggtgcc tggctcggcg tcctggacgg acaccgtcaa ctgctcggga atcgacagcg
    13741 cctcggcctc gatctcgatg gtggtggttt cctgggtgac caaggtatcg gaagcggcct
    13801 cgccctcgac caccacggtg acttccacga cgaccttctc accgcgacgc acgaccagca
    13861 ggtccgcgtg ctggatggtg cggcggatcg ggtgaatgtc gagcgctttg gtcagggcca
    13921 gctgttcctt gccgccgatg ttgagcgtca gcaccgcgtt ggtgcccgag tgccgcagca
    13981 ccgccgcgaa gtcgtgcccg ggcaattcca ggtgctgtgg atcggcgccg tggccgtaga
    14041 ggaccgcggg aatgttgccg gcgcgccggg ctcgccggga cgcgcccttg ccggtctcgg
    14101 ttcgcaccgt aaccgccaac tggttgactg tgcctttagc catttcgtcg ctcctgtgtg
    14161 ctctcgttcg gggcacggcc gggatcgcga ctaagagtcg gtctccgtcg ataacggtgc
    14221 cggcctaatg accggccacc ctcgccgtga cgctcggtca ggttagcgca tgtgcacctc
    14281 agacggaaaa tttggcgccc gggggtagcg gcgaatgtca catctgtcac ctgggcgtct
    14341 ggcggcgcgt tggctcatca aaaatgcctg ctcgctttgc gagagaccgg tgtggccgac
    14401 gtggccgatg acgtcagagc gggaatttgg tgccggtgag ctgctcggac agctcccaga
    14461 gcgaggtggc ggcagccgcg tctcgtgccc gccggctgcg cccgacgggt tgggtggggc
    14521 cgatatagcc gaatcgaggc ccgacaaagg tgtcgcccgg taagtcctgc gacgccgcgt
    14581 acaaggtttg ccgggcaccg aaatccgcgt cggtggccac cacccgggtc gccgccgaga
    14641 tcagcgcgtc actcaccctg cggcccgagg cgccttgcag gttcgtgtgc gagtagccgg
    14701 gatgtgccgc cagggcgcgc agcggggacc cgaccgagtc gagccgtcgc tgcagctcgc
    14761 tggtgaataa caggttggcg agcttcgact ggccgtaggc cgcccagggc gagtagcgcc
    14821 gcgatctcca gttcaggtcg tcgaagtcga tgcggcccgg ccaatgcgcc atcgacgaca
    14881 ccgtgacgac ccggtcggtg agcttgggta gcaacaggtt ggtgagcgcg aaatgaccga
    14941 gatggttggt gccgatctga ctctcgaagc cgtccacggt cagcgcgtag ggcgcggcca
    15001 tgatgccggc gttgttgacc aatatgtcga ccccgtccac cccgtcagcg aactgacgga
    15061 ccgacgacag gtcctgcagg tcgagtcgat gaacctcgat ggtgccggtc atctgctggg
    15121 cagcggcctc gcccttggcc gtgttgcgga cggccaggat gaccctggcg ccgtttcggg
    15181 ccagctcgcg ggccgtcacc gcgcccagcc cgctgttggc gccggtgatg atggcggtgc
    15241 gccccgcgaa agatggcagg tctgcggcgg tccagtcagt catggctcac cacactagcg
    15301 acgccggcgg ttgtcacttc gcggcgacga cgaatccgcg aatgatcgcc tcgatgtccg
    15361 atgactgcgc gaccgcctgg tcggccagac tggtgatcgt cagctgaacc aggtaccgct
    15421 ggttagccgg cggcgatccg gtggggatga cgatccggtt ccagctgtgc agccgggtgc
    15481 cgtcgaggtc gtagctgccc tgaatcatcg acgaggggaa gccgttgtag tccgccgtcg
    15541 aggcgtccag ctgcttgaag ttctcgaaca gctgggcatc gtcattgccg tgcttgatga
    15601 ccttggccgg gtcgaaatcc ccacgcaacg cgaagaccac cagccttgcc gtcgggtact
    15661 tacggtcttt ggcgatgatc agcgtctccg gcgtgatctt cgggttggtg aacggcgacc
    15721 agcccggtgg cgtgggtatc gacacggcca gatcggtcag ggagcctggc gccacctgtt
    15781 tcccaatgac gccgatgtct tccagatact gcgacagggg aacgggcttg tccgtcgtgg
    15841 tggttgtggt ggcggccgct gtcgagctct tcgacaagat cgactggtag tcgggcgctt
    15901 tcggcgcgca cgcacccgcg accagcacga ccaccgcggc ggccatgggc aggagtctca
    15961 cagaatctcg cggaccgtat cgatgggccg cgccagcctg gtgcccttcg gcgtgacgac
    16021 gaaaggtcgc tcgatcagga tggggtgctc ggccatcgcg tcgagcaact cgtcatcggt
    16081 cacgtcggcg aggttgagtt cggtgtagag cgcttcgcgc ttgcgcaccg ccgtccgcac
    16141 gtcgatgccg gcgtcgtgga tcatgcgaac caactcggcc cgcgacggcg gagttttcag
    16201 gtactggacg atggtcggct caacaccgtt gtcccgcaac agatccagcg tcttgcggga
    16261 ggtgctgcat ttcgggttgt ggtagatgac ggtgttgtgt gaagtctccc ctgttacggg
    16321 agccatctag gcgtctccgt cgaatagccc tgtgactgag ccattttcga agacggcgcg
    16381 aatggtgctg gccagcagcg gcgcgatgga caagacggtg agctggggga agcgcttctc
    16441 ctcgccgatc gggagtgtat tcgtgacgat cacctcccgg gcgccgcacg ccgccaaccg
    16501 ctgcgcggcc ggatcggaca gcacaccgtg agtcgccgcg atgaccacgt cgctggcacc
    16561 ggcctcacgc agcagcttca ccgcgccggc gatggtgccg ccggtgtcga tcatgtcgtc
    16621 gatcagcacg caggtccggc ccgctacttc accgacgacg cggttggaga ccacctggtt
    16681 gggcacgcgc gggtcgcggg tcttgtggat gaaggccagc ggaacgccgc ctagcgagtc
    16741 ggcccatttc tcggcgatgc gcacccggcc ggagtcgggg gagaccacga ccatgttgcc
    16801 gtccggatag ttgtccttga tgtagccggt cagcaggttc tggccgcgca tgtggtcgac
    16861 gggcccgtcg aagaaaccct ggatctggtc ggtgtgcagg tcgacggtca cgatcc
//
LOCUS       DASSGQ010000034         5904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_549551,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000034 DASSGQ010000000
VERSION     DASSGQ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549551"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFI55_01565"
     CDS             <1..397
                     /locus_tag="VFI55_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET7072756.1"
                     /translation="VGAAAYGVGVLMRPPPRQTVAEQIMSAPDVRTVSRPLANGTATV
                     MFSRDRNEGMLVMNNVPPPSPGTVYQMWLLGANGPRSAGTMGTAAVAPSTTATLSNLG
                     ASTALAFTVEPGTGSPRPTGAILAELPLS"
     gene            complement(389..1255)
                     /locus_tag="VFI55_01570"
     CDS             complement(389..1255)
                     /locus_tag="VFI55_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072757.1"
                     /translation="MRFAVTFLLWLATTVALAVAVPAVWAQLTIVGEDGWAALAEKAA
                     ADPALQSAAAAELAARATALIAAHGGGRYPVDGAQLHDVAGAFTAGPSFPPLFAQANR
                     EAHRWLFSAPQSGQNGNQWVIDLAPMLNDASFQPILSTYHVQEPATLTVPLTVSIPST
                     GQSVRQGQLNRLATWGPWVSSGAVALSGLCGLLTVAVARRRGKALAGLGISALLVGAA
                     GWAGIEVGGRYVDDALNTTTGDIRQIADVMVRHAEAGMHQWLNLTLAAGAALAGLGVL
                     VAMAGSVRRPST"
     gene            complement(1276..1791)
                     /locus_tag="VFI55_01575"
     CDS             complement(1276..1791)
                     /locus_tag="VFI55_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol transferase"
                     /protein_id="HET7072758.1"
                     /translation="MSGTDAAAQELLRDAFTRLIEHVDELTDGLTEEVSNYRPATDAN
                     SIAWLIWHSARVQDIQLAHVAGVEEVWTRDGWVDRFRLDLPRHDTGYGHNSQQVAKVR
                     APSDLLSGYYHAVHKLTLEYIASVTADELARVVDTSWDPPVTASARLVSIVDDCAQHL
                     GQASYVRGIAP"
     gene            complement(1802..2185)
                     /locus_tag="VFI55_01580"
     CDS             complement(1802..2185)
                     /locus_tag="VFI55_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7072759.1"
                     /translation="MIDHTGINCADYPRSQEFYDAVLGVLGFTRQMDFGRAIGYGRDG
                     KPAFWISDGAAGAGLGPNREIHLAFEAADENAVRAFHQVAVGLGAESLHAPRLWPEYH
                     PGYFGAFVRDPDGNNVEAVCHNLRA"
     gene            2233..3963
                     /locus_tag="VFI55_01585"
     CDS             2233..3963
                     /locus_tag="VFI55_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7072760.1"
                     /translation="MTYDLIIRNGTIVDGLGGEPYVGDVAVKDGAIEAVGSVNGDSAT
                     REIDATGLLVTPGFVDLHTHYDGQSIWSQRLTPSSAHGVTTVVMGNCGVGFAPCRKED
                     HDVLVDVMAGVEDIPGVVMTDGLPWNWETFPEYLDTLAAGKRDIDVAAYLPHSPLRVY
                     VMGQRGADREPATPEDLAKMRALAKEAMEVGALGFASSRLTIHKTESGSPIPSYDAAR
                     EEIEEIARGVVDGGGGLLQFVPDIPAGGYQPVLQTVFDVAEDVGLPVTFTLVVANAGD
                     PTWPDAITMVEKANAAGGDITAQLLPRPIGLIIGLQLSANPFVLYPSYREIAHLPLAE
                     RVAEMRKPEVRARILADKPGQGHPILYVAQMWDWIFPLDDNPDYEPDPSDSIGARARA
                     KGVNPMEEAYDRLLDDDGRAMLLVATSNLENNSLDTVGRLLHRDDVVLGLGDGGAHYG
                     MICDASYSTYFLAHWARDRKSGRFSVAEAIRKLTSVPARVAGLGDRGRIAVGYKADIN
                     VIDHAALRLHKPMISYDLPAGGRRLDQTADGYVATIVSGEIIAENGVPTDARPGELIR
                     GRKPSPVPVQ"
     gene            4002..5411
                     /locus_tag="VFI55_01590"
     CDS             4002..5411
                     /locus_tag="VFI55_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015629539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPE family protein"
                     /protein_id="HET7072761.1"
                     /translation="MTSPHFAWLPPEINSALLFAGPGAAPLLAAAAAWDGLAEDLASS
                     ASSFFSVTSDLASGSWQGASAAAMMAVSTQYVSWLSAAAAQAEAASSQAAAIAAAFES
                     AVAATIQPAVVAANRALVQALSATNWFGLNAPAIMDTEAAYEAMWAADVAAMSGYHAD
                     ASAAVAQLAPWQQILQKLGINFNKGQLTFGAPGSTQSSSNAGFGNSGTQNMGSGNSGN
                     NNLGMGNLGNNNVGFGNTGNNDFGIGLTGNNQFGFGGFNSGSGNIGLFNSGTGNVGFF
                     NSGNGNFGIGNAGTSNSGFLNSGAVNSGFFNNGNANAGFFQDNTLAGQAGSDPGMFNS
                     SRYLTGGVSAANLSSGLLNSVTATTGGLNPGLLNPALANAAFAAHADPGVIAGSVEPG
                     GANAGVVSAANGSTPTPSAMRTTTTAPSGFFTAGNDAGFRNPAATRESSIPNSGFFNK
                     ADRDPGAPNPGIRLPDLPD"
     gene            5450..5899
                     /locus_tag="VFI55_01595"
     CDS             5450..5899
                     /locus_tag="VFI55_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET7072762.1"
                     /translation="MRSKKKVDVKRLAAALTDYPFAYLITVDDGYRVHTVTVAPELRS
                     LPESPDGPAAVLDIGLVGGHTRENLAHRSDVTLLWPPSEPGGYSLIVDGIAEAQSGSD
                     AGSEAGSETVRLGVVPTRALLHREADPDSPTAAKGCKHDCVVFALPA"
BASE COUNT          983 a         2052 c         1969 g          900 t
ORIGIN      
        1 ggtgggtgcc gcagcgtatg gcgtcggggt gctgatgcgg ccgccgccaa ggcagacggt
       61 cgccgagcag attatgtcgg cccccgatgt gcggacggtc tcgcgcccgc tggccaacgg
      121 cacggccacg gtgatgttct cccgcgaccg caacgagggg atgctggtga tgaacaacgt
      181 gccgccgccg tcccccggca ccgtctatca gatgtggttg ctcggagcca acggcccgag
      241 atcagcgggc accatgggga ccgcggcggt ggcgccgtcg accacggcga cgctgtccaa
      301 cctgggagcc tccacggcgc tggcgttcac cgtcgagccg ggcaccggat cgccccggcc
      361 gaccggggcg atactggccg agttgccgct aagttgacgg gcgccgcacg ctgcccgcca
      421 tcgctacgag cacgccgagc cccgccagcg ccgcccccgc ggccaacgtc aggttgagcc
      481 attgatgcat gccggcctcg gcgtgccgca ccatcacgtc ggctatctga cggatgtctc
      541 cggtggtcgt gttgagcgcg tcgtcgacgt agcggccgcc gacctcgatg cccgcccatc
      601 cggctgcgcc gacaagcagt gccgagatgc cgagaccggc cagcgccttg ccgcggcggc
      661 gcgcgaccgc aacggtcagc aacccgcaga gtccgctcag ggccaccgcg ccgctgctca
      721 cccagggccc ccaggtggcg agcctattga gctgtccctg ccgcaccgat tgccccgtag
      781 acggaatcga caccgtcagc ggaacggtca gcgtcgcggg ctcctgcacg tggtaagtgc
      841 tcaagatcgg ttggaacgac gcgtcgttga gcatcggggc caggtcgatc acccattgat
      901 tgccgttctg cccggactgc ggggcgctga acagccagcg gtgcgcctca cggttcgcct
      961 gggcgaacaa cggcggaaac gacgggccgg ccgtgaacgc gccggcgacg tcgtgcagct
     1021 gcgcgccgtc gacggggtag cgtcccccgc cgtgtgcggc gatgagcgcc gtcgctcggg
     1081 cggcgagttc ggccgccgcg gccgattgca gagccggatc ggccgccgcc ttctcggcca
     1141 gcgcggccca gccgtcctcg ccgacgatgg tcagctgcgc ccacaccgcc gggaccgcca
     1201 ccgcgagggc caccgtggtg gccagccata gcaggaaggt caccgcgaat cgcacttggc
     1261 caatctacgg ccaaattacg gtgcgatccc ccgcacgtag gaggcctgcc cgaggtgttg
     1321 tgcgcagtcg tcgacgatgc tcaccaatcg cgcgctggcg gtcaccggcg gatcccagct
     1381 ggtgtccacg acgcgcgcca gttcgtcggc ggtcacgctg gcgatgtatt cgagcgtcag
     1441 cttgtgcacc gcgtggtagt agccggacag caggtcggag ggtgcccgta ccttcgccac
     1501 ctgttgggag ttgtggccgt agccggtgtc gtggcgcggc aggtccagcc tgaaccggtc
     1561 cacccagccg tcacgggtcc acacctcctc cacgccggct acgtgggcca gctgaatgtc
     1621 ctgcacgcgg gcgctgtgcc agatcagcca ggcgatgctg ttggcgtcgg tggccggccg
     1681 gtaattggac acctcctcgg tcagcccgtc ggtgagttcg tcgacgtgct cgatcaaccg
     1741 ggtgaacgcg tcgcgaagca actcctgagc cgcggcgtcc gtgcctgaca tacgtgcgac
     1801 cctacgcccg caagttgtgg cagacggctt cgacgttatt gccgtcggga tcgcgcacaa
     1861 aagcgccgaa atagccgggg tgatactccg gccagagccg cggcgcgtgc aacgactccg
     1921 cacccagacc caccgcgacc tgatggaatg cgcgcaccgc gttctcgtcg gcggcctcga
     1981 aggcgaggtg aatttcccgg ttaggtccca ggccggcgcc ggcagcacca tccgagatcc
     2041 agaacgcggg cttgccgtcc cgcccgtagc cgatggccct gccgaaatcc atctgtcgcg
     2101 tgaagccgag cacgcccagc accgcgtcgt agaactcctg cgatctcggg tagtccgcgc
     2161 agttgattcc ggtgtggtcg atcacaacac cgatcctggc atgacccaac gacacggtcg
     2221 tagattattt agatgaccta cgacctcatc atccgcaatg gcaccatcgt cgacgggctg
     2281 gggggtgagc cgtacgtcgg cgacgtggcg gtaaaagacg gcgccatcga agcggtcggt
     2341 tccgtgaacg gcgattccgc gacacgggag atcgacgcga cggggctcct ggtcacacct
     2401 ggcttcgttg acctgcacac ccactacgac ggccagtcca tctggtcgca gcgcctgacc
     2461 ccgtcgtcgg cgcacggggt caccacggtg gtgatgggta attgcggggt cggctttgca
     2521 ccgtgtcgca aggaagacca cgacgtgctc gtcgacgtga tggccggggt cgaggacatt
     2581 cccggcgtgg tcatgaccga cgggctgccg tggaactggg agaccttccc ggaatacctg
     2641 gacactttgg cggcgggcaa acgcgatatc gatgtcgcgg cctatcttcc gcattccccg
     2701 ctgcgggtgt acgtgatggg ccagcgcggc gccgaccgcg agccggccac ccccgaagac
     2761 ctcgcgaaga tgcgggcgct ggccaaggag gcgatggagg tcggggcgct gggcttcgcg
     2821 tcgtcgcggt tgacgatcca caagaccgag agcggttcgc cgatcccgag ctacgacgcc
     2881 gcccgcgagg agatcgaaga gatcgccagg ggtgtcgtcg acggcggcgg cggcctgctg
     2941 caattcgtgc ccgacattcc ggccggcggc taccagcccg tgctgcagac ggtgttcgac
     3001 gtcgccgagg acgtgggact gcccgtcacg ttcaccctgg tcgtcgccaa tgcgggcgac
     3061 cccacctggc cggacgccat caccatggtc gaaaaggcaa acgcagcagg tggagacatc
     3121 accgcgcagt tgctgccacg ccccatcggg ctgatcatcg ggctgcagct gagcgccaac
     3181 ccattcgtgc tctacccgag ctaccgcgag atcgcgcacc tgccgctggc cgagcgggtg
     3241 gccgagatgc gcaagcccga ggtccgcgcc cgcatcctgg ccgacaagcc cggccagggt
     3301 cacccgatcc tgtacgtggc ccagatgtgg gattggatct tcccgctgga tgacaacccc
     3361 gattacgagc ccgacccgtc ggacagcatc ggagcccgcg cccgggccaa gggtgtgaac
     3421 ccgatggagg aggcctacga ccggctgctc gacgacgacg ggcgcgccat gctgctggtc
     3481 gcgacgagca acctggagaa caactcgctg gacaccgtcg gccggttgct gcaccgggac
     3541 gacgtggtgc tcggcctcgg cgacggcggc gcgcactacg gcatgatctg tgacgccagt
     3601 tactcgacgt acttcttggc gcattgggcg cgcgaccgca agtccgggcg gttcagcgtc
     3661 gccgaggcga tccgcaagct cacctccgtg ccggcccggg tggccgggct gggtgaccgc
     3721 ggccgcatcg ccgtcggcta caaggccgac atcaacgtca tcgaccacgc cgcgttgcgg
     3781 ctgcacaagc cgatgatcag ctatgacctg cccgccggcg ggcgacgcct ggaccaaacc
     3841 gccgacggat atgtggcaac gatcgtgtcc ggcgagataa tcgccgaaaa cggcgttccc
     3901 accgatgcac gtcccggcga gctgatccgc ggccgcaagc ccagcccggt acctgttcaa
     3961 taacgccacg aagggcgggt cgtcccggct aagttgggtt ggtgacgagc ccgcattttg
     4021 cgtggttgcc gccagaaatc aactcggcac tactcttcgc cggccccggt gccgcgccgc
     4081 tgcttgctgc ggcggcggca tgggacgggc ttgccgagga tctggcgtcg tcggcgtcgt
     4141 cgtttttctc ggtgacgtcg gacctggcca gcggttcgtg gcagggggcg tcggcggcgg
     4201 cgatgatggc ggtctcgacg cagtatgtga gctggctcag cgcggcggcg gcgcaggccg
     4261 aagctgcgtc aagccaggcc gcggccatcg cggcggcgtt cgaaagcgcc gtggcggcca
     4321 ccattcagcc cgcggtggtg gcggccaatc gtgccctggt gcaggcgctt tcggcgacga
     4381 actggttcgg actcaatgcc ccggcgatca tggacaccga ggccgcctac gaggcgatgt
     4441 gggcagcgga tgtggccgca atgtccggct accacgccga cgcgtcggcg gccgtcgcac
     4501 agctggcgcc ctggcagcaa atcctgcaga agctcggtat caatttcaac aaaggccaac
     4561 tcactttcgg cgccccaggg tccacgcaaa gcagttcgaa cgctggtttc ggaaacagcg
     4621 gcacgcagaa catgggctcg ggcaactcgg gcaacaacaa cctcggcatg ggcaacctcg
     4681 gcaacaacaa cgtcggtttc ggcaacaccg gcaacaacga cttcgggatc gggctgaccg
     4741 gcaacaacca gttcggcttc ggcggcttca actcgggtag cggcaacatc ggcctgttca
     4801 actcgggcac cgggaacgtc ggctttttca actccggcaa cggaaacttc ggcatcggaa
     4861 acgcgggcac ctccaacagc ggcttcttga actctggggc ggtcaactcg ggctttttca
     4921 acaacggcaa cgccaacgcc ggcttcttcc aggacaacac cctggcaggc caggccggct
     4981 cggacccggg catgttcaac tccagccgct acctcacggg cggcgtcagc gcggcgaacc
     5041 tcagctcggg cctgcttaac tcggtcaccg caacgaccgg cggcttgaac ccgggcctgc
     5101 tcaacccggc gctggcgaac gccgccttcg ccgcccacgc cgacccgggc gtcattgccg
     5161 gcagtgtcga accgggcggc gcgaacgccg gcgtcgtcag cgcggcgaac ggctccaccc
     5221 ccacgccatc ggcgatgcga accacgacaa ccgccccgtc gggcttcttc accgccggca
     5281 acgacgcggg tttccggaac ccggccgcca cccgtgagtc gtcgatcccc aactcgggct
     5341 tcttcaataa ggcagaccgc gacccgggcg ctccgaaccc tggcatcagg ctgccggacc
     5401 tgcccgacta gcggcccacg agcctgtatt actcgaatat cctcgaacca tgcgctcgaa
     5461 gaagaaggtg gacgtcaagc ggctcgccgc cgccctcacc gactacccgt tcgcgtacct
     5521 gatcaccgtc gacgacggat atcgcgtaca caccgtgacg gtcgctccgg agctgcgcag
     5581 tctccccgaa agccccgacg ggcccgcagc ggtcctcgac atcgggctgg tcggcgggca
     5641 cacccgggaa aacctcgcac atcgcagcga cgtcaccttg ctgtggccgc cgtccgagcc
     5701 ggggggctac tcgctgatcg tcgacggcat agccgaagcg cagtccggct cggacgccgg
     5761 ctccgaggcc ggctcggaga ccgtccgcct gggcgtggtg cccacccgcg cgctgctgca
     5821 ccgcgaggcg gatcccgact cacctacggc ggccaagggc tgcaagcacg actgcgtggt
     5881 ctttgcgctg ccggcctgag acac
//
LOCUS       DASSGQ010000035         6228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_701339,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000035 DASSGQ010000000
VERSION     DASSGQ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701339"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1199
                     /gene="bioB"
                     /locus_tag="VFI55_01600"
     CDS             156..1199
                     /gene="bioB"
                     /locus_tag="VFI55_01600"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HET7072763.1"
                     /translation="MTQAPIRPTTDAEQMNNADIVSVARQQVLERGEGLSSEQVLQVL
                     RLPDDRLDELLALAHEVRMRWCGPEVEVEGIISLKTGGCPEDCHFCSQSGLFASPVRG
                     VRLDIPSLVEAAKQTAKSGATEFCIVAAVRGPDERLMAQVAAGIEAIRNEVEINIACS
                     LGMLTAKQVERLSAMGVHRYNHNLETARSFFPNVVTTHTWEERWETLSMVRDAGMEVC
                     CGGILGMGETLEQRAEFAADLAELGPDEVPLNFLNPRPGTPFGDREVMPVTEALKSVA
                     AFRLALPRTMLRFAGGREITLGDLGAKQGILGGINAVIVGNYLTTLGRPAEADLELLA
                     DLQMPIKALNASL"
     gene            1213..1449
                     /locus_tag="VFI55_01605"
     CDS             1213..1449
                     /locus_tag="VFI55_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072764.1"
                     /translation="MAEKVSGPGAPVSAGVYNVYTGERGGTAVPAAARLGLEPPRFCA
                     ECGRRMVVQVRPDGWRVQCSRHGQADSAELEAQR"
     gene            1533..2108
                     /locus_tag="VFI55_01610"
     CDS             1533..2108
                     /locus_tag="VFI55_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2567 domain-containing protein"
                     /protein_id="HET7072765.1"
                     /translation="MLAAAATGGFIAGVWAWIAPPIHAVVAITRAGERVHDYLGTESE
                     HFFDAPCLMLGLLTLVAVVAPVLAWQWHERRGPAMVICLSIGTIIAAGVASAIGALVM
                     RLKYGALNFDAVPLSGRPSVAYVIQAPPVFLSDDPLLVAATLMWPAGIAALVYALLAA
                     ADARDDLGALTPADQSHALPMAPEAPEAAVS"
     gene            complement(2147..3487)
                     /locus_tag="VFI55_01615"
     CDS             complement(2147..3487)
                     /locus_tag="VFI55_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HET7072766.1"
                     /translation="MVELGNLAGKRGAQWIGRPNHEELQRKVRPLLPSDDPFYQPPPG
                     FQHAEPGTVLRSRDVELAFLGLIPQPTTAVQLLYRTMDMNGEPEATVTTVIVPAGLSS
                     WQTCPLVSYQCAIDAMTSRCFPSYALRRRAKALGSLSQFEFLLINAAVAEGWAVSLPD
                     HEGLHGSWGAPYEPGYRVLDGIRAALSSDRVGLSPSAQIGLWGYSGGGLASAWAAEMC
                     GEYAPELDIVGAVLGSPVGDLGHTFRRLNGGVLAGLPALVVAALVHIYPDLDRVIKEH
                     ASDEGRALLEALENMTTVEAVVRMAGKDMGDYLDEPLEDILSTPEVTYVFDSIKLGTA
                     VPTPPVLIVQAVHDYLIDVKDIDELADAYSAGGAQVTYHRDAFNEHICLHPLSAPMTL
                     RWLTDRFAGRPLDAHLVRTTWPTMFNPMTYVGMARLAKIAAKVITGRKVPRRPL"
     gene            complement(3685..4395)
                     /locus_tag="VFI55_01620"
     CDS             complement(3685..4395)
                     /locus_tag="VFI55_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7072767.1"
                     /translation="MAHINTAHEVLAVVFQVRQVTGALRTEKPQLNVLLWQRARDPQR
                     GTWSLPGGRLRNDEDLTTSVRRQLAEKVDLRELAHLEQLAVFSDPHRVPGARVIASTF
                     LGLVPSPATPELPPDTRWHPVNALPPMAFDHGPMVTHARTRLVAKMSYTNIGFALAPR
                     EFALSTLRDIYGAALGYQVDATNLQRVLARRGVITATGTIAQSGRSGGRPAALYHFAD
                     AALRVTDEFAAFRPPGPA"
     gene            4442..5491
                     /gene="nadA"
                     /locus_tag="VFI55_01625"
     CDS             4442..5491
                     /gene="nadA"
                     /locus_tag="VFI55_01625"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HET7072768.1"
                     /translation="MTVLTRMDTLADDMTAGITNTPTGYVGLDGNEQWANEIRRLAKL
                     RGATVLAHNYQLPAIQDVADHVGDSLALSRIAADAPEDTIVFCGVHFMAETAKILSPD
                     KTVLIPDQRAGCSLADSISPDELRAWKDEHPGAVVVSYVNTTAAVKALTDICCTSSNA
                     VEVVESIAPDREVLFCPDQFLGAHVRRVTGRKNVHVWAGECHVHAGINGDELTDQARA
                     HPDAELFVHPECGCATSALYLAGEGTFPPDRVRILSTGGMLEAAHRTHARKVLVATEV
                     GMLYQLRRAAPQIDFQAVNDRASCKYMKMITPAALLRCLVEGADQVHVDPEIAAAGRR
                     SVQRMIEIGQPGGGE"
     gene            5491..>6228
                     /locus_tag="VFI55_01630"
     CDS             5491..>6228
                     /locus_tag="VFI55_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET7072769.1"
                     /translation="MIRPDWTHAADVVVIGTGVAGLAAALAARRAGRSVVVLSKADHT
                     RGATATHYAQGGIAVVLPDNDDSVDAHVADTLAAGAGLCDPDAVSSIVSDGYLAVSEL
                     VGVGARFDESAPGRWDVTREGGHSRRRIVHAGGDATGAEVQRALDHAAAVLDLRTGHV
                     ALRVLHDGTAVTGVLVGNADGYGIVSAPAVILASGGLGHLYGATTNPEGSTGDGIALA
                     LWAGVAVSDLEFIQFHPTMLFAAAGGEV"
BASE COUNT          990 a         2044 c         2161 g         1033 t
ORIGIN      
        1 tggtgacata ccccggcggt ccccacgttc tgcggccgtt acccttcttg gaacaccgtt
       61 tcgttctggc tgccatgcca cgtggcaccg aggtgggctg atgcgtttcg catcggccct
      121 ggacaaagat gacaaagatg cagggggagt acctggtgac ccaagcgccg attcgaccga
      181 cgaccgacgc cgagcagatg aacaacgcgg acatcgtgtc ggtggcacgg cagcaggtgc
      241 ttgagcgcgg tgagggactg agctccgaac aggtgctgca ggtgctccgg ctgcccgacg
      301 accggctcga cgagctgctg gcgttggccc acgaggtgcg gatgcgctgg tgtggtcccg
      361 aggtcgaggt cgagggcatc atcagcctga agaccggcgg atgcccggaa gactgccact
      421 tctgctcgca gtcggggctc ttcgcgtcgc cggtgcgcgg cgtccggctc gacattccca
      481 gcctggtcga ggcggcgaag cagactgcca agtcgggtgc caccgagttc tgcatcgtgg
      541 cggccgtacg tggacccgac gagcggttga tggctcaggt cgcggcgggc atcgaggcga
      601 tccgcaacga ggtggagatc aacatcgcct gctcgctggg gatgctgacc gccaagcagg
      661 tggagcggct ttccgcgatg ggcgtgcacc gctacaacca caacctggag accgcccgct
      721 cgttcttccc caacgtcgtg accactcaca cctgggaaga gcgctgggag acgttgtcga
      781 tggtgcgcga tgccggcatg gaggtgtgtt gcggcggcat cctcggcatg ggcgagacgc
      841 tggagcagcg cgcggagttc gccgccgacc tcgccgaact cggccccgac gaggtgccgt
      901 tgaactttct caacccgcgg ccgggcaccc cgttcgggga ccgggaggtg atgccggtca
      961 ccgaggcgtt gaagtcggtg gccgcgttcc ggctggcgct gccccgcacg atgctgcgat
     1021 tcgccggggg tcgcgagatc accctgggtg acctgggcgc caagcagggc atcctgggcg
     1081 gcatcaacgc cgtgatcgtc ggaaactacc tgaccacgct cggccggccc gccgaagcgg
     1141 acctggaact gctcgcggac ctgcagatgc cgatcaaggc gctgaacgcg agcctgtaag
     1201 aaactgggag ccgtggctga aaaggtgagc ggtccgggcg ctccggtcag cgcaggcgtg
     1261 tacaacgtct acaccgggga acgaggtggt accgccgtgc cggcagcggc ccggctgggc
     1321 ctcgagccgc cgcggttctg cgccgaatgc ggacgccgga tggtggtcca ggttcgtccg
     1381 gacggctggc gggtgcagtg ctcgcggcac ggacaggcgg actcggccga attggaggca
     1441 cagcggtgac cgagcaggcc gcgcccgagg ctcccgggcc caccggcgtc ccccgcacgt
     1501 ccccttcctg gaggcgcgcg gtcgtcgtca cggtgctggc agcggccgcg actggtgggt
     1561 ttatcgctgg ggtgtgggca tggatcgccc cgccgatcca cgccgtggtg gccatcacgc
     1621 gcgccggcga gcgagtccac gactatctgg gcaccgagtc cgagcacttt ttcgacgcgc
     1681 catgcctgat gctcggcctg ctgaccctgg tggcggtagt cgctcccgtg ctggcgtggc
     1741 aatggcatga gcgccgggga ccggcgatgg tgatctgcct gtcgatcggc acgatcattg
     1801 ccgccggcgt cgcttcagcg atcggggcgc tggtgatgcg gctgaaatat ggcgcgttga
     1861 acttcgatgc cgtaccgctg tcgggtaggc cgtcggtggc gtatgtcatc caggccccgc
     1921 cggtgtttct ttccgatgat ccgttgctgg tggcggcgac gctgatgtgg cctgcgggca
     1981 tcgccgcgtt ggtctatgcg ctgttggcgg cggcggacgc tcgcgacgac ctgggcgcac
     2041 tcacgccagc cgatcagtca catgccttgc cgatggcgcc ggaggccccg gaagccgctg
     2101 tgtcatagcg gagctttaga ggccccatag cggacctata gcggacctac aagggacgac
     2161 gcgggacttt cctgccggtg atgaccttgg ccgcgatctt ggccagccgc gccatgccga
     2221 cgtaggtcat cgggttgaac atcgtcggcc atgtggtccg gacgaggtgc gcgtccagtg
     2281 gtcggcccgc gaatcggtcg gtgagccagc gcagcgtcat cggggcggac agcgggtgca
     2341 ggcagatgtg ttcgttgaac gcgtcccgat ggtaggtgac ctgggcgcca ccggccgaat
     2401 acgcgtcggc gagctcgtcg atgtccttca cgtcgatgag gtagtcgtgc acagcctgca
     2461 cgatcaacac cggcggcgtc ggcaccgcgg tgcccagctt gatgctgtcg aagacgtacg
     2521 tgacctcggg cgtcgacagg atgtcttcga gcggttcgtc caagtagtcg cccatgtctt
     2581 tgcccgccat gcggacgacc gcttccacgg tggtcatgtt ttccagcgcc tcgagcaggg
     2641 cgcggccctc gtcgctggca tgttctttga tcacccggtc gaggtcgggg tagatgtgca
     2701 ccagcgcggc caccaccagc gcgggcagac cggccagcac gccgccgttg agcctgcgga
     2761 aggtgtgacc gaggtcgccg acgggcgatc ccagcacggc accgacgatg tccaattcgg
     2821 gtgcgtactc gccacacatt tcggcggccc aggcgctggc cagcccgccg ccggagtatc
     2881 cccacagccc gatttgcgct gacggtgaca gcccgacccg atcggagctc agcgcggcgc
     2941 ggatcccgtc caggacgcgg tagccgggct catacggcgc gccccacgaa ccgtgcaggc
     3001 cttcgtggtc cggcagcgag acggcccaac cctcggccac ggccgcgttg atcagcaaaa
     3061 actcgaactg gctcaatgac ccgagagcct tcgccctgcg ccgcagcgcg taggacggaa
     3121 agcagcgtga cgtcatcgcg tcgatcgcgc actggtagga caccagcggg caggtctgcc
     3181 acgaggagag cccggccgga acgatcaccg tcgtcaccgt cgcctcgggc tcgccgttca
     3241 tgtccatcgt tcggtacagc agctgaacag cggttgtggg ctgaggtatc agcccgagaa
     3301 acgccagctc gacgtcgcgt gagcgcaaca ccgtcccggg ctcggcgtgc tgaaaaccgg
     3361 gtggcggctg gtagaaaggg tcatccgacg gcagcaacgg ccgcaccttg cgttgcagct
     3421 cctcgtggtt cggccggccg atccactgcg cgccacgttt tccggcgaga ttgccgagct
     3481 caaccattga gggtcccttc ttggctatcc ggcattgtcg cgtatgaacg gacggtaacc
     3541 aaagcgtgat cgccgtattg tgagccaatt ctcgtctgtg aattgagctc tcgtctgtga
     3601 attgagctca ccgactccgg tgttgccgcc caatctaccg gcgtgtccgg ggcaggggtt
     3661 tgcgttcgcc cagccgggcg gcgttcaggc cgggccgggc ggtcgaaacg ccgcgaactc
     3721 gtcggtgacc cttagcgccg cgtcggcgaa gtggtacagc gccgcaggtc gcccgccgct
     3781 gcggccggac tgcgcgatgg tgccggtcgc cgtgatgact ccgcggcggg ccaaaacccg
     3841 ttgcaggttc gtcgcgtcga cctgatagcc cagcgccgcg ccgtagatgt cgcgcaacgt
     3901 ggacagcgcg aattcccttg gtgctaaggc gaatccgatg ttggtgtagg acatcttggc
     3961 gaccagcctg gtgcgcgcgt gggtgaccat cggaccgtga tcgaacgcca tcgggggcag
     4021 cgcattcacc gggtgccaac gcgtatcggg cggcagctcg ggggtggcgg gggagggcac
     4081 cagacccagg aacgtcgacg cgatcacccg ggcgcccggc acccggtgtg ggtcggagaa
     4141 cacagccagt tgctccaagt gagcgagctc acggaggtcc accttctcgg ccaattggcg
     4201 ccgcacggag gtggtcaggt cctcgtcgtt gcggagccgg ccacccggta gtgaccacgt
     4261 gccgcgttgc ggatcccggg cacgttgcca taacaacacg ttcagctgcg gtttttctgt
     4321 tcggagggcc ccggtaactt gacgaacctg gaacacgacc gcgagaactt cgtgggcggt
     4381 gttaatatgg gccatgtttt cgattgtaag tcgaaaacct ttcgacgcga aaggacccgc
     4441 catgacggtt ttgactcgca tggacacact cgcggacgac atgactgccg gcatcacgaa
     4501 cacgcccacc ggctacgtag gtctggacgg gaatgagcag tgggccaacg agattcgccg
     4561 cctggcaaag ttgcgcggtg cgaccgtgct cgcgcacaac taccagctgc ccgccatcca
     4621 ggacgtcgcc gaccacgtgg gcgattcgct ggcgctgtcg cggatcgccg ccgacgcacc
     4681 cgaggacacc atcgtgttct gcggcgtgca cttcatggcc gagaccgcca agatcctcag
     4741 tccggacaag accgtgctca tcccggacca acgggccggc tgctcgctgg ccgattcgat
     4801 cagccccgac gaactgcgtg cctggaagga cgagcacccc ggcgctgtcg tcgtttccta
     4861 cgtcaacacc acggccgccg tgaaggcgct caccgacatc tgctgcacct cgtctaatgc
     4921 ggtcgaagtg gtcgagtcca tcgccccgga ccgcgaggtg ctgttctgcc cggaccaatt
     4981 cctcggggcg cacgttcgcc gcgtgacggg ccgcaagaac gtccatgtct gggccggcga
     5041 atgccacgtg cacgcaggca tcaacggcga cgaactcacc gaccaggccc gcgcgcatcc
     5101 cgacgccgaa ttgttcgtgc acccggaatg cggatgtgcc acgtcggcct tgtacctcgc
     5161 cggcgaaggc accttccccc ccgaccgggt gaggattctg tcgaccggcg ggatgctgga
     5221 ggccgcgcac cggacgcacg cccgcaaggt tctggtcgcc accgaagtcg gcatgttgta
     5281 ccagctgcgc cgggcggcgc cgcaaatcga cttccaggcc gtcaacgacc gcgcctcgtg
     5341 caagtacatg aagatgatca ccccggccgc gttgttgcgt tgtcttgtcg agggtgctga
     5401 ccaagttcac gtcgaccccg aaatcgcggc ggcgggccgg cgcagcgtgc agcgcatgat
     5461 cgagatcggg cagccgggcg gcggcgaata atgatccgtc ccgattggac ccatgcagcc
     5521 gacgtcgtcg tcatcggcac gggcgtcgca ggattggccg ccgcgctggc cgcccgacgc
     5581 gccggccgca gcgtggtcgt cctgtccaag gcggaccaca cgcgcggggc gaccgccacc
     5641 cactacgcac aaggcggcat cgccgtggtg ctgccggaca atgacgactc ggtggacgcc
     5701 cacgtcgccg acaccctggc ggcgggcgcg ggcctctgtg atccggacgc ggtgagctcg
     5761 atcgtctccg acggctacct tgccgtgtcc gaattggtcg gcgtcggagc ccgtttcgac
     5821 gaatcggccc cgggtcgctg ggacgtgacg cgcgagggtg gtcactcgcg gcgacgcatc
     5881 gtgcacgcgg gcggcgacgc caccggcgcc gaggtccagc gcgcgctcga ccacgccgcg
     5941 gccgtgctgg acctccgcac cggccacgtg gccctgcggg tgctgcacga cggtacggcg
     6001 gtgacgggcg tgctggtggg caacgcggac gggtacggga tcgtcagcgc gcccgcggtg
     6061 atcctggcgt ctggcggcct cgggcacctc tacggcgcga ccaccaatcc cgagggctcc
     6121 accggcgacg gaatcgcgtt ggcgttgtgg gccggcgtcg cggtcagcga tctcgagttc
     6181 atccagttcc acccgacgat gcttttcgcc gctgccggcg gcgaggtc
//
LOCUS       DASSGQ010000036        24170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_124302,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000036 DASSGQ010000000
VERSION     DASSGQ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 24170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24170
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124302"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VFI55_01635"
     CDS             complement(<1..216)
                     /locus_tag="VFI55_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072770.1"
                     /translation="MKVAVSNQYSAAAKSAAAPNATITPGGDMTPASGAFDTELSRTS
                     RSTGHTEYFSISPLLYSFPVSVRGNCVS"
     gene            131..1054
                     /locus_tag="VFI55_01640"
     CDS             131..1054
                     /locus_tag="VFI55_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET7072771.1"
                     /translation="MSPPGVIVAFGAAADFAAAEYWLETATFTLTEDPSVDRRAVTVD
                     SVPEAVAELAERCQRWPHASSVCEDVLRAVDPSGPTLAGVVTESLAYSTLQAGPEFAR
                     WLDERGPARMPEIADPVQAQRAGDTLRIRFNRPQRHNAFSTDARAALLEALTVAQLDP
                     SVTGIVLSGNGPSFCSGGDLAEFGTFADPASAHLARTRHSPALALDALTARLGRSCRA
                     EVHGRVMGSGLEMAAFCGWIEARDDSVFGLPELGLGLIPGAGGTVSITRRIGRWRTAY
                     LVLSGHSVDADTALAWGLVDATYVGPQTVSQ"
     gene            complement(1048..1476)
                     /locus_tag="VFI55_01645"
     CDS             complement(1048..1476)
                     /locus_tag="VFI55_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014712517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD225/dispanin family protein"
                     /protein_id="HET7072772.1"
                     /translation="MSYPPGPYEGSPEWQGQQQPHWQPQQPPWQGPPPAGWPGPQPGW
                     PGQREPDNYLVWAILCTVLCCIPLGIVSIVYSTKVSGLWAQGRYAEAQSASDSAKKWA
                     IIGAVVGIVLDVILVMLWFFVFAVAISKLPSTTTTTFSGY"
     gene            complement(1605..2756)
                     /locus_tag="VFI55_01650"
     CDS             complement(1605..2756)
                     /locus_tag="VFI55_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7072773.1"
                     /translation="MQVTDEEDIVVATVRAFIDRDVKPGVREVEHANTYPEAWIEQMK
                     RIGIYGLAIPEEYGGSPVSMPCYVRVTQELARGWMSLAGAMGGHTVVAKLLTLFGTEE
                     QKRAYLPRMATGELRATMALTEPGGGSDLQNMSTTALPDGPDGLVINGSKTWISNARR
                     SGLIALLCKTDPTAAPRHTGISIVLVEPGPGLTVSRDLPKLGYKGVESCELSFDDFAV
                     PASAVLGGRMGEGFAQMMKGLETGRIQVAARALGVATAAFEDALAYAQQRESFGRPIW
                     QHQAIGNYLADMATKLTAARQLTRYAAERYDSGQRCDMEAGMAKLFASEAAMEIALNA
                     VRIHGGYGYSTEYDVERYFRDAPLMIVGEGTNEIQRNVIAAQLIARGGI"
     gene            2808..3824
                     /locus_tag="VFI55_01655"
     CDS             2808..3824
                     /locus_tag="VFI55_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET7072774.1"
                     /translation="MTGNGYAGQLEGGPYFDDLSVGQVFDWAPSITLSSGLAAAHQAV
                     VGDRLRLSLDAGLCAAVTGLPAPLVHPALVCDVAIGQSTLVTQRVKANLFYRGLTFHR
                     FPIVGDSIYTRTEVVGLRANSLKQGRAPTGLAALRMITIDQADRLVLDFYRCAMLPAS
                     PDWDPSGAPGDDLSAIGADAAPPVADPTAQWDGDVFRNKVPGPHFATGLAGAVLRSTG
                     DVVSSAPELARLTLNIAATHHDSRVGGRRLVYGGHTIGLALAQASRLLPNLVTVLGWQ
                     SCDHTGPVHEGDTLYSELHVESAEPTDHGGALRLRSLVYAVGESDGEPDRPVLDWRFD
                     ALHF"
     gene            3894..5099
                     /locus_tag="VFI55_01660"
     CDS             3894..5099
                     /locus_tag="VFI55_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET7072775.1"
                     /translation="MSSAGSQWASKWGSSGLAHLTGLGDGPPDFCRANVLARAEQVAT
                     AVGHRMGIAIDAAGLLIGRAALLGLTRGGRVSAGGATRLLAARDGWCAITLSRPDDIA
                     AVPALVQVDDPPADPWPLLRHWVATHPVSTITERSRLLDIPAAALGEVAAGRPLIRRM
                     GPRASERGLTGLLVADLSSMWAGPLCGKLLARGGATVVKVESPARPDGTRAGNRAFFD
                     WINGEKLSYCVDFVTQADELRALLAVADVVIEGSRPAALARRGLGPDHAASHAGRIWL
                     RINGYDEARPAFGDDAAVAGGLVGTAVGAGSHGPVFCGDAIADPLTGLDATLTVTESL
                     GRGGGELIHVSMAAVAATYAALPTADSVAGFPAPPPQAPPASRPAPELGADNDAVRRL
                     VVERGCPSC"
     gene            5093..6397
                     /locus_tag="VFI55_01665"
     CDS             5093..6397
                     /locus_tag="VFI55_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014943093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7072776.1"
                     /translation="MLIQRAALLDGTIADIRVGAHIDEVGEGLVARRGEGVLYAGGGT
                     VLPGLHDHHVHLRSAASALNSFLVGPPGVSTKAQLTQLLCNASPGPDGWIRAVGYHES
                     VAGELDRAALDGLVRNIPVRIQHRSGALWILNSAALGRIGLAEHPDGRLRSADRGWSE
                     ALARRETDLAELSRRITATGVTGVTDATPDLHADDMVSLVVAHRRDEFRPRLRFLSPG
                     KQILHDDRLDLDSLTGWIADQHADDRPVAVHCVTAAQLVVTIAALRAAGSHPLDRIEH
                     AAVVPDDQVSDLAALGVTVVTQPNFVAERGDQYLAEVPVAEHDQLWRVATLLNATVPV
                     AFSTDMPFGHGDPWTAMRAAVYRTTPSGAVLGANERVSALRALTMFLGRPDRPGRVRT
                     VETGQPGDLCVLTEPPQTVLAELDAGMVAATVIGGELVYFAM"
     gene            6691..8808
                     /locus_tag="VFI55_01670"
     CDS             6691..8808
                     /locus_tag="VFI55_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017836322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HET7072777.1"
                     /translation="MAASTPQPTKTHCPYCSLQCGIELDVSGGSVVLAPQDDFPTNRG
                     GLCAKGWTAAELLGHPDRLTKPLVRDGRREPLRPATWNEALERVVTAFQKAQHDHGRD
                     AVGCFGGGGLTNEKAYQFGKFARVALRTSAIDYNGRFCMSSAAAASIRSFGVDRGLPF
                     PLADIAHADLLLIVGSNPAETLPPAMQFFDEGRSRGAKHIVVDPRRTATAARADLHLQ
                     PVPGTDLALANGMLNVVVREGLIDQKYIADRTTDFEAVRRAVRLYWPDRVERLTGVPE
                     NDIVAAARMLAKAERAIILTARGAEQHSCGTDTVQGFINLALALGLPGGEYQGFASIT
                     GQGNGQGGREHGQKCDQLPGYRKLDDPAARAHVAQVWGINPADLPSSGRSAYEMLDAI
                     GTPGGVRALWVMASNIVVSTPNSLRVAAKVADLDFLVVSDIFLSETAALADVVLPTAQ
                     WAEESGTMTNLEGRVILRRKAVAAPGEVRSDLDVMADLAKRLGVQGFSADPEEVFDEL
                     TRASAGGKADYRGISYARIDAEQGVFWPCPSPSYPATVRMFAQDFPTPDRRAHFHPVE
                     QAAPAERPDDEFPYYLTTGRVLRQYQSGTQTRRVASLAEAEPEPVVELHPMLAQLVGV
                     RTGEKVRLRTRRGEVVMTARTESGIRPDTVFAPFHWGGAGNINRLTIAELDPHSRMPS
                     FKVCAVALSRADTADTRKRADDG"
     gene            8801..9154
                     /locus_tag="VFI55_01675"
     CDS             8801..9154
                     /locus_tag="VFI55_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase"
                     /protein_id="HET7072778.1"
                     /translation="MGDDSTPKFLQGVFTFDGKGYDAPFLLDSTLRYVVPAGVISQPL
                     YFRGGNSADELITVIVMRDGAPMRYFPIGAKDAVHVPLRVVEDLIADTVLEVFVTAPD
                     GLSGTVIVDIGLVEV"
     gene            9161..11683
                     /gene="nirB"
                     /locus_tag="VFI55_01680"
     CDS             9161..11683
                     /gene="nirB"
                     /locus_tag="VFI55_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005172397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HET7072779.1"
                     /translation="MTLLAQPSTNGHARPVPARLVMVGNGMAGARALEEIIARGGRER
                     FAITVFGDEPYGNYNRILLSNVLAGSDDAREIYLNPLDWYTENNIDLRAGVRVVRIDP
                     FAHVVFANDGTKIRYDKLILATGSRSFFPPMEGIWQADNKTLIPGVFGFRSLDDCEAM
                     IEFAKGRSHGLVVGGGLLGLEAARGLQNRGLQAGVVQSGPTLMNAQLDDQGGAILRRL
                     VETVGIAVHTGKRPTSVITEGALPVAMGFDDGTRLPCDMVVLAAGIRPNVGLAQRAGL
                     TVERAIVVDDQMRSIDDDDIYVVGECAQHRGQVYGLVAPLWEQAGVLADHLAGTNPRA
                     AYHGSRTATKLKVAGVDVAAMGLKGPENADDEFVQYVEPKHGVYKTLVVRDDKLVGAT
                     LVGDVSKVAFLMQAFDRGLPLPPERVSLMFDIGTPDVAVGAAELSDDAQVCNCNGVSK
                     AAIVNCIKQGQTSLTGVMNATRAGKGCGSCKGLVGQIVEWAVSESGAEVCEDVASSWY
                     VPGVPYEKPELMRLVRELNLHSVSSVFEALAPEGREDAQSKMALSSLLEMMWGDEFVD
                     ERASRFINDRVHANIQRDGTFSVVPQLKGGVTTSEQLRKIADVADKYAIPMIKLTGGQ
                     RIDLLGVRKEDLPAVWADLGMPSGYAYGKSFRTVKTCVGSDFCRYGLGDSTALGIAIE
                     ERYQGLASPAKMKLAVTGCPRNCAEALCKDLGVVAIEGGQWQIYVGGAAGAHVRKGDL
                     LATVDSHHEVMTLTGRFLQYYRENANWLERTYTFVPRIGIERIRAIVVDDANGIAAAL
                     DARMQKSVEAYRDPWAQSRDEPEDNQFRPSLPLIPLPQVPVR"
     gene            11680..11985
                     /locus_tag="VFI55_01685"
                     /pseudo
     CDS             11680..11985
                     /locus_tag="VFI55_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
     gene            12060..13508
                     /locus_tag="VFI55_01690"
     CDS             12060..13508
                     /locus_tag="VFI55_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013416711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HET7072780.1"
                     /translation="MTAQPATTAPAATTATPAPPLSRLRAGHHRISGWDPEDLQAWQN
                     GGSKVARRNLVWSIFAEHIGFSVWSIWSVMVLFMPQNAYHIDAAGKFYLVAVPTLVGA
                     VLRLPYTTAVARFGGRNWTIVSALALLVPLTLTWWFMVHPGFSYTTCLIVAAFAGLGG
                     GNFASSMTNINAFYPARLKGWALGLNAGGGNIGVPVIQLVGLLVIATVGNRSPQIVCA
                     TYLVLVGLAGLGAALFMDNLDNQKSNAAAMLRVIRFADTWLISFLYIGTFGSFIGYSF
                     AFGQVLQINYLSVPHTTAAAAALHASQIAFLGPLLGSLSRPLGGRLADRIGGAKITMA
                     CFVAMVLAAGVLVVAGTRGDAHPGKISHGTMVAYVVGFILLFLISGIGNGSVYKMIPA
                     IFAAKSLDLKNVSAVETLNWERSMAGALIGIAGAIGALGGVGINVVLRASYAGAQKSA
                     TTAFWVFLVFYIVCVGVTWAAYVRPRGATSLV"
     gene            13712..14374
                     /locus_tag="VFI55_01695"
     CDS             13712..14374
                     /locus_tag="VFI55_01695"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014943091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7072781.1"
                     /translation="MLGHLYDRALGGERCWIRHEDGEVLPLPAHRWLGARGISDGSDP
                     SADALEEAFDEAVTQLCTGPTIELGCGPGRLVARLTRRGIPALGIDRSATAVRLAGCG
                     GAPALLGDVFAPLPGTGLWQTVLLVDGNVGLGGDPRRILARAAELLGCGGCCVAEFDA
                     EVIGICPRWVRLESAGDVGPWFRWASVGVDSAATLAAHVGLTLTGVRLIAGRVVAILA
                     AA"
     gene            14380..15657
                     /locus_tag="VFI55_01700"
     CDS             14380..15657
                     /locus_tag="VFI55_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7072782.1"
                     /translation="MDRHPPPGVPRRTRVLVHFRSPLRGPWLTSVFGLVLLITLPIVI
                     VTGLLSYIAYAPQLGQAIPGDVGWLRLPAFTWPTRPSWLYRLTQGLHVGLGLAIIPVV
                     LAKLWSVIPRLFVWPPARSIAQLLERLSLLMLVGGVLFEIVTGVLNIQYDYVFGFSFY
                     TAHYFGAWVFIAGFLMHICLKIPRMVTGLRSLPLREVLRTNRSDTRPQSPDPDGLVAG
                     DPAEPTMSRRGALALVGSGVLLVCTLTVGQTLGGAARGAALLLPRGRGPGGLRGDFPV
                     NKTAAAAGITPESVGGGWLLTLRGGPSEVALDRATLAALPQYTARLPIACVEGWSTVQ
                     TWSGVRLAELARIAGVPAPRAARVTSLQRGGAFGAATLQANQIRDPAALLALRVNGAD
                     LSLDHGYPARIIVPALPGVHNTKWVASIEFEPS"
     gene            15659..16225
                     /locus_tag="VFI55_01705"
     CDS             15659..16225
                     /locus_tag="VFI55_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072783.1"
                     /translation="MPGLVTRFRACYGSHPLHLLTMLSGFALLGAVLTIFKPATLWNP
                     GAWWQSIAVWLAACVVAHDLLLFPLYALADRVLFTRIGRRPSPVRHPKVSARNHIRVP
                     ALGAGLTLLIFLPGIIQQGAPTYQAATGQTQQPFLGRWLLLTAAMFAVSALCYVARLA
                     VCGRRARRGDDLAASVGFGDPPGSTLRT"
     gene            16222..17427
                     /locus_tag="VFI55_01710"
     CDS             16222..17427
                     /locus_tag="VFI55_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7072784.1"
                     /translation="MTARGPRVTPRIRWLLRAGPADYILAFSMAGASLPVVGKRLEPL
                     GGLTAMGVWGARQAPQALSAMTQEWLTPGSGDVRRRDRESTQEVARAALRGVVSAADL
                     DVEWPTAERTPPIWDAMSQRRYLYRRGVHYGNSRAQVLDVWRLKELPAQPAPVLVFVP
                     GGAWVHGRYLGQGSALMSRLAEQGWVCLAIDYRVAPHHRWPRHITDVKTAVAWARANV
                     DKFGGDRNFVAVAGCSAGGHLAALAGLTPDDPALRAELPDGADTSVDAVVGIYGRYDW
                     HDRSTPERVRFVDFLERVVVRRTIARHPEVFRDASPIARVHRNAPPFLVIHGSKDGVI
                     PVDQARSFVERLRAVSHSVVGYLELPGAGHGFDLIDGERAGAMAHATALFLNEVYRTR
                     TRIGAKEVI"
     gene            17472..18881
                     /locus_tag="VFI55_01715"
     CDS             17472..18881
                     /locus_tag="VFI55_01715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HET7072785.1"
                     /translation="MKRLSGWDAVLLYSETPNVHMHTIKVAVIELAPDRRDFDVDAFR
                     QVIGGRLNKLEPFTYQLVEIPFKFHHPMWREHCEIDLDYHIRPWRLPAPGGRRELDDA
                     IGRIASTPLDRERPLWEMYFVDGLADNRVAVVGKIHHALADGVASANLMARGMDLVPT
                     PESGPQVTDPAPSKPQLVTSAFMDHLRHLGRIPATIRYTADGMGRVRSSSRRLSAELT
                     RPFTPPPTFMNHLLTPERRFATATLALSDVKETGRKLGATINDMVLAMSAGALRTLQL
                     RYDGTAQPLLASVPVSFDFSPERISGNRFTGMLVALPTDSDDPLERVRCSRENAISAK
                     ESHHLMGPELVSRWAAYMPPAGTEAFFRWASGRDGQNKILNLNISNVPGPRERGRVGG
                     ALVTEIYSVGPLTAGSGLNITVWSYVDQLNISVLADGATVKDPHEVTEAMVADFIEIR
                     RAAGLSDELTVVETAMAQA"
     gene            19023..19814
                     /locus_tag="VFI55_01720"
     CDS             19023..19814
                     /locus_tag="VFI55_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HET7072786.1"
                     /translation="MSTGNADASVGEPEVLVEQRDRILIITINRPKAKNAVNGAVSRG
                     LADAVDRLDDDAGLSVGILTGAGGSFCAGMDLKAFTRGENVVVAGRGLGFTERPPVKP
                     LIAAVEGFALAGGCELALATDLIVASRDSAFGIPEVKRGLVAAGGGLLRLPDRIPYAI
                     AMELALTGDQLPAERAHELGLVNVLAEPGKVLEAAIALAEKITANGPLAVAATKRIIT
                     ESRGWSPDTMFTEQIKIFAPVFSSNDAKEGAIAFAEKRPPHWTGT"
     gene            complement(19853..20443)
                     /locus_tag="VFI55_01725"
     CDS             complement(19853..20443)
                     /locus_tag="VFI55_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET7072787.1"
                     /translation="MSEAQRPGRWRTGQQNRQRIIDAARERFMRDGYEGATVRAIAAD
                     AGVDVAMVYYFFDSKEGLFTAATLTGPEHPLHQLATLLDAGTEQIGPRLVRRFLEHWE
                     EGAVFEPFLTLWRSAAIHPQARKVLHDSLAGPIAKRVAAEFGVADAELRVELVASHLA
                     GLAFARYQLKIEPLASTAIDDLVAWLGPTVQRYLTS"
     gene            20547..21104
                     /locus_tag="VFI55_01730"
     CDS             20547..21104
                     /locus_tag="VFI55_01730"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014712511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET7072788.1"
                     /translation="MVTNTRERRLWWRHLMSVLLFPVTVTVVVPALIVIPAGLRGPQL
                     GVTATVPRVIVGSLLIAAGLGLLIWTVVLFDRVGEGTLGLGNVMGEPVHLVVRGPYRH
                     VRNPMITGVLCILLGEAAITASGSLLLWFAIFLTFQATAIRFWEEPHLARRYGSEYVD
                     YRRNVPRWIPRISAWSAGHTGTSRC"
     gene            21177..22712
                     /locus_tag="VFI55_01735"
     CDS             21177..22712
                     /locus_tag="VFI55_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HET7072789.1"
                     /translation="MSISLLLEMAASSNPDRTAIVSAEPSGPVRLTTQQLSDLADGGA
                     AVIAGSNAKHVAYVGAGGAMLPVLIFAAARAGLAFTPINYRLSDDGIQALIRRLPEPL
                     VIVDGRYRITLGDASDRVIESDDFLAAARNADPSDYAVEFPDPESVAIVLFTSGTTSE
                     PKAVELTHNNLTSYVTGTVEFESADPADAALICVPPYHIAGVGAALSNLYAGRKMVYL
                     PTFDPLEWVRLINAEHVTTATVVPTMLERIVTVLEAGGHRLPSLRNLAYGGSKVGLPL
                     VRRALGLLPNVGFVNAYGLTETSSTIAVLTPDDHRAAQAALSSSDIALARRLGSVGQP
                     VPGIELQIRGDDGTVLGAGQTGELFVRGEQVSGRYTGVGSVLDENGWFPTKDIAMLDE
                     EGYLFIGGRSDDTIIRGGENIAPAELEEVLIEHPHVRDVAVVGVEDPQWGQAIVAVVV
                     PAAGVEPDAEELREYVRGSLRGSRTPDRVVFRGELPTTATGKVLRREIIETLQEDAKR
                     TSA"
     gene            22709..23005
                     /locus_tag="VFI55_01740"
     CDS             22709..23005
                     /locus_tag="VFI55_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072790.1"
                     /translation="MIKNGTRLASQVCDTQVIVVRSAESLDDLRCGGAPMVAIGAERS
                     GSLDPAFAGGTVMGKRYVVETGAEVLVTKPGAGSLSVGATALQLKEVKPLPASD"
     gene            complement(23012..>24170)
                     /locus_tag="VFI55_01745"
     CDS             complement(23012..>24170)
                     /locus_tag="VFI55_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HET7072791.1"
                     /translation="AAANPWQTLQQDVLNAINAPTELLLQRPLIGNGTNGLPGTGQNG
                     QAGGILWGNGGNGGSGAANSPGGAGGAAGLFGHGGTGGAGGLSTTAGVAAGNGGAGGA
                     GGLVFGNGGAGGAGGSYNNSTAFGPAGLGGAGGDAIGLFGTGGAGGVGGLGNVPQVFA
                     GGIGGAGGHGGHGGLLYGNGGSGGNAGDGSFGSVGGGGGVGGLLSGNGGDGGNGSGVQ
                     SGGTGGNAGLIGGGGRGGNGTGLSSAGFGGPGGPGGNGGILWGDGGDGGNGAQSTLGR
                     GGAGGAGGNAIGLFGNGGDGGGAGQTNAAITGATGGHGGNGALLIGNGGAGGSAGTGP
                     QPGNGGDGGNAQLIGNGGAGGDSFGAGSTVGQGGAGGLLLGVPGPDGQTGQ"
BASE COUNT         3675 a         8205 c         8282 g         4008 t
ORIGIN      
        1 cgagacgcag ttgccgcgca ccgacaccgg aaagctgtac aagagcgggc tgatcgagaa
       61 gtattcggtg tgacccgtgc tccggctggt ccgactgagt tcggtatcga atgcgccgga
      121 cgccggcgtc atgtcacccc cgggtgtgat cgtcgcgttc ggcgcggccg ccgacttcgc
      181 ggcggccgaa tactggttgg agacggcgac tttcacgctt accgaggatc cgtctgtcga
      241 tcgtagagcg gtcaccgtcg attcggtgcc cgaggccgtg gccgagctgg ccgagcgctg
      301 ccaacggtgg ccgcacgcca gcagcgtgtg cgaggacgtg ctccgcgcgg tggacccgag
      361 cggcccgacg ctggccggag tggtgacgga gtcgctggcc tactcgacct tgcaggccgg
      421 cccggaattc gcgcgctggc tcgacgagcg tggcccggcg cgcatgcctg agatcgccga
      481 cccggtgcag gcgcagcgcg ccggcgacac cctgcggatc aggttcaacc gcccgcaacg
      541 ccacaacgcc ttttccaccg atgcgcgcgc cgcgctgctc gaggcgctga ccgtcgcgca
      601 actggacccg tcggtgaccg gaatcgtgtt gagcggcaat ggaccgtcgt tttgcagcgg
      661 cggagacctc gccgaattcg gcaccttcgc cgacccggcg agcgcgcacc tggcccgcac
      721 tcggcacagc cccgccctgg cgctggatgc cctgaccgcc aggctcggcc ggtcctgccg
      781 cgccgaggtg cacggccggg tgatgggcag cggactggag atggcggcgt tctgcggatg
      841 gattgaggcg cgcgacgatt cggtgttcgg gctacccgaa ttgggtctgg gcctgatccc
      901 cggcgccggc ggcaccgtaa gcatcacccg ccggatcggc cgctggcgca cggcatatct
      961 ggtgttatcg ggtcactcgg tcgacgcgga taccgcgttg gcctgggggc tggtggacgc
     1021 gacttacgtt ggcccccaaa ctgtttctca gtagccagaa aacgtcgtgg tggtggtgga
     1081 cgggagtttc gagatggcca ccgcgaagac gaagaaccac agcatcacca ggatcacgtc
     1141 gaggacgatg cccacgacgg cgccgatgat ggcccacttc ttggcgctgt cggacgcgga
     1201 ttgggcctcg gcgtagcgcc cttgggccca cagcccggag accttggtgg agtacacgat
     1261 cgagacgatc ccgagcggaa tgcagcacag gaccgtgcac agaatcgccc agacgaggta
     1321 gttgtccggc tcgcgctggc ccggccagcc cggctgcggg cccggccagc cggcgggcgg
     1381 ggggccctgc cacggcggct gctggggctg ccaatgcggt tgttgctggc cctgccattc
     1441 gggtgaacct tcgtacggcc ctgggggata gctcatggcg gctgccttcc tgtttggttc
     1501 ccacgggtcc gaaaaggtct gctcccgagc cgatttgctg gcgtgagaag tcaggcaaat
     1561 atacagcacg tgcccaggcc gggccgggac attgtgattc gggctcagat cccgccgcga
     1621 gcgatgagct gcgcggcaat cacgttgcgc tgaatctcat tggtgccttc accgacgatc
     1681 atcaacggcg cgtcccggaa gtagcgctcg acgtcgtatt cggtcgaata gccatagccg
     1741 ccgtggatgc gcacggcgtt cagggcgatc tccatcgccg cctccgaagc gaacagcttg
     1801 gccatcccgg cctccatgtc gcagcgctgg ccactgtcat agcgttcggc ggcatagcga
     1861 gtgagctgac gggcggcggt gagtttggtc gccatgtcgg ccaggtagtt gcctatcgcc
     1921 tggtgttgcc agatcggccg gccgaagctt tcccgctgct gcgcataggc cagcgcatcc
     1981 tcgaatgccg ccgtcgccac gcccagggcc cgggcggcga cttggatgcg acccgtctcg
     2041 agccctttca tcatttgcgc gaacccctcg cccatccggc cgcccagcac cgccgacgcc
     2101 ggcacggcga agtcgtcgaa cgacagctcg caggactcga cgcccttgta gcccagcttg
     2161 ggcaggtccc gcgacactgt caggccgggc ccgggctcaa ccagcacgat cgaaatgccc
     2221 gtgtgacgcg gcgcggcggt ggggtcggtc ttgcacagca gcgcgatcag ccccgaacgg
     2281 cgcgcgttgc tgatccaggt cttggagccg ttgatgacca gaccgtccgg gccgtccggt
     2341 agtgcggtgg tcgacatgtt ctgcaggtcc gagccgccgc ccggttccgt cagcgccatg
     2401 gtggcccgca gctcgccggt cgccatgcgc ggcaggtagg cccgtttctg ctcctcggtg
     2461 ccgaacagcg tgagcagctt ggccaccacg gtgtggccgc ccatcgcacc ggccaggctc
     2521 atccagcccc gcgccagctc ctgggtgacc cgcacatagc acggcatcga caccggcgac
     2581 ccaccgtact cctcgggtat ggccaggccg tagatgccga tccgcttcat ctgctcgatc
     2641 cacgcctcgg ggtaggtgtt ggcatgctcg acctcgcgga cacccggctt gacgtcgcgg
     2701 tcgatgaagg cccgcacagt ggcgaccacg atgtcttctt cgtcagtcac ctgcatccac
     2761 cctccaacat tgacccccga ttcgacaccc tgccagcatg gggcgttgtg actggtaacg
     2821 ggtatgcggg tcagctcgag ggcgggccct acttcgacga tctttccgtg gggcaggtat
     2881 tcgactgggc gccgtcgatc acgctgtcat ccggtctcgc cgcggctcat caggcggtcg
     2941 tgggggaccg cctgcggctg tcgctggacg ccggtctgtg tgcggccgtg acgggcctgc
     3001 ccgccccgct ggtgcatccg gcgctggtct gcgacgtggc tatcggtcag tcgacgctgg
     3061 tgacccagcg ggtcaaggcc aacctgttct atcggggcct cacgtttcac cgattcccga
     3121 ttgtcggcga ctcgatatac acccgtaccg aagtggttgg gctgcgggcg aactcgctca
     3181 agcagggtcg ggcgccgacc gggttggcgg cgttgcggat gatcacgatc gaccaggccg
     3241 accggttggt gctcgacttc taccgatgtg ccatgctgcc ggccagcccg gattgggatc
     3301 cgtcaggcgc acccggcgac gacctgtcgg ccatcggcgc cgacgcggcc ccaccggtcg
     3361 ccgatccgac ggcgcaatgg gacggcgatg tcttccgaaa caaggtgccc ggtccccatt
     3421 tcgcgaccgg cctggcgggt gcggtgctgc gcagcaccgg cgatgtggtc agtagcgcac
     3481 ccgaactggc tcgactcacc ctgaacatcg ctgccacaca ccatgattcg cgggtcggcg
     3541 ggcggcgttt ggtgtacggc ggacatacca tcgggctggc actagcgcag gcgagtcggc
     3601 tgttgccgaa tttggtgacg gtgctgggtt ggcagtcctg cgaccacacc ggcccggtgc
     3661 acgagggtga caccctctac agcgagctgc acgtggagtc cgccgaaccg accgatcacg
     3721 gcggggcgtt gcggctccgg tcgctggtat acgccgtcgg tgagtccgac ggtgaacccg
     3781 accggccggt gctggactgg cggtttgacg cgctgcattt ctgaacggtt cgcgagcgta
     3841 acgccactga gaaaccgccc gccatttttc gcagcggcgt tacgctggcg gccgtgagct
     3901 cggcggggtc gcaatgggcg tccaagtggg gtagcagcgg gctggcccac ctgacggggc
     3961 tgggcgacgg gccacccgac ttctgccgcg cgaacgtgct ggcccgcgcc gagcaggtgg
     4021 ccacggcggt cggtcaccgc atgggtatcg ccatcgacgc cgccggcctg ctcatcggac
     4081 gcgccgcact gctcggattg acccgcggcg gccgggtgtc ggccggcggc gccactcggc
     4141 tgcttgcggc gagggacggc tggtgtgcga tcacgctgtc gcgccccgac gacatcgccg
     4201 ccgtccccgc gctggtgcag gtcgatgacc cgcccgccga cccgtggccg ctgctgcggc
     4261 actgggtcgc aacacatccg gtgtccacga tcactgaacg ctcacgcttg cttgacatcc
     4321 cggccgccgc cctgggcgag gtcgccgccg ggcggccgct gattcgccga atgggcccgc
     4381 gggcgtcgga gcgcgggctg accggcctgc tggtcgccga cctgtcgtcg atgtgggcgg
     4441 gcccgctgtg cggaaagctg ctggcacgcg gcggggcgac cgtcgtcaag gtcgaaagcc
     4501 cggcacgccc ggacggcacc cgagcaggca accgcgcgtt cttcgattgg atcaacggtg
     4561 aaaagctctc ctactgcgtc gatttcgtta ctcaggccga cgagctgcgc gctttgctcg
     4621 ccgtcgccga tgtcgtgatc gaaggctcgc gtcccgcggc gctggcccgg cgcgggctcg
     4681 ggcccgacca cgccgcgtca cacgcgggtc gaatctggct gcggatcaac ggatatgacg
     4741 aagcgcgacc ggcattcggc gacgacgccg ccgtggccgg cggcctggtc ggcaccgccg
     4801 tcggtgccgg ttcgcacgga ccggtgttct gcggcgacgc catcgccgac ccgctgaccg
     4861 ggctggacgc gaccttgacc gtgacggagt cgctcggccg gggcggcggc gaattgatcc
     4921 acgtgtcgat ggccgccgtc gccgcgacct acgccgcact gccgacggcg gactcggtgg
     4981 cgggcttccc ggcgccgccg ccgcaggcac cgcccgcgtc ccgcccggcg cccgaactcg
     5041 gcgccgacaa cgacgccgtg cggcgcttgg tcgtcgaaag aggttgcccg tcatgctgat
     5101 tcaacgagct gcgttgctgg acggaacgat cgccgatatc cgggtcggcg cgcacatcga
     5161 cgaggtgggg gagggccttg ttgccaggcg cggcgagggt gtgctctacg cgggcggcgg
     5221 aaccgtgctg cccgggctgc acgaccacca cgtgcacctg cgctcggcgg cttccgcgct
     5281 caattcattt ctcgtcggac cgcccggggt cagcaccaaa gcgcaactga cacaactgct
     5341 gtgcaacgca tcgccaggtc ccgacggatg gattcgcgcc gtcggctatc acgagtccgt
     5401 ggccggcgag ttggaccggg ccgctctcga cgggttggtg cgcaacattc cggtgcgcat
     5461 ccagcaccgc agtggtgcgc tgtggatcct caactccgcc gcactgggcc ggatcggcct
     5521 ggccgaacat cccgacggcc ggctgcgcag cgccgaccgc ggctggtcgg aggcgctggc
     5581 gcggcgcgaa accgacctgg cggaactcag ccgccgcatc accgcgaccg gcgtcaccgg
     5641 ggtcaccgac gccacccccg accttcatgc cgacgacatg gtctcgctgg tggtggcgca
     5701 ccgtcgcgac gagtttcggc cccggctgcg tttcctgtca ccgggcaagc aaatcctgca
     5761 cgatgaccgt cttgacctgg actcgctgac gggctggatc gccgatcagc acgcggacga
     5821 ccggccggtg gccgtgcact gcgtgaccgc ggcgcagctg gtggtgacca tcgcggcact
     5881 gcgtgccgcc ggcagccatc cgctcgatcg catcgaacac gccgccgtcg tgcctgacga
     5941 ccaggtgagc gacctcgccg cactcggggt cacggtggtg acacagccca acttcgtcgc
     6001 cgagcgcggc gaccagtacc tcgccgaggt ccccgtcgcc gagcatgacc agctatggcg
     6061 cgtcgcaaca ctgctcaacg cgacggtgcc ggtggccttc tccaccgaca tgccgttcgg
     6121 tcacggagac ccatggacgg cgatgcgcgc tgccgtatac cgcaccacac cgagcggcgc
     6181 cgttctcggt gcgaacgaac gtgtctcggc gctaagggct ttgacgatgt ttctggggcg
     6241 gccggatcgt ccgggccggg tgcggaccgt ggaaacggga cagccagggg acctctgcgt
     6301 gctgaccgag ccaccgcaga cggtgctggc cgaactggac gccggcatgg tggcggccac
     6361 cgtcatcggc ggcgagctgg tgtacttcgc gatgtgactc gcgatcgcgc aagtcgccca
     6421 acgctaccca atccggataa ccggtgtatg actggactgt tacaaccaat gacccacgcc
     6481 gcgtatgaga ttcgttgaca gctcgcgaaa acatttgact caccagcggc gtgagcgcgc
     6541 ggtagtgctg cggacaatca gcggaaatgc cttggaaaca cttggcgcgt agcttcgccg
     6601 acaggggacg aatcgatcgg aggtgaagtg gcaggtcaag aagaaggcgg ccgcacctcc
     6661 gccgagacag caacgcagcg gtgatggttc atggcggcca gcacgcccca gccgacgaaa
     6721 acgcactgtc cgtactgctc gttgcagtgc ggaatcgaac tcgacgtgtc cgggggatcc
     6781 gtggtcctcg caccgcagga cgactttccg accaatcgcg gcgggctgtg cgctaaaggc
     6841 tggacggcgg ccgagttgct gggccacccc gatcggctca ccaagccgct ggtccgcgac
     6901 gggcgccgcg aaccgctccg gccggcgacc tggaacgagg cactggaacg cgtcgtcacc
     6961 gccttccaga aggcgcaaca cgaccacggc cgtgacgcgg tgggatgctt cggcggcggc
     7021 ggcctgacca atgaaaaggc ctatcaattc ggcaaattcg cgcgcgtcgc actgaggact
     7081 tcggcgatcg actacaacgg ccggttctgc atgtcgtcgg cagcggcggc gtcgatccgc
     7141 tccttcggtg tcgaccgggg gttgccgttc ccactggccg acatcgcgca cgccgacctc
     7201 ctgctcattg tcggcagcaa ccccgccgaa accctgccgc cggcgatgca gttcttcgac
     7261 gagggccgct cccgcggcgc caagcacatc gtcgtcgatc cacgccgcac cgccaccgcc
     7321 gcccgcgccg acttgcacct gcaaccggtc cccggcaccg atcttgcgct ggccaacggc
     7381 atgctgaacg tcgtcgtccg ggaaggactg atcgaccaga agtacatcgc cgaccggaca
     7441 acggatttcg aagcggtgcg ccgcgcggtt cgactctact ggcccgaccg ggtggaacgg
     7501 ctcaccggcg tgcccgagaa cgacatcgtg gcagcagccc ggatgctggc gaaagccgaa
     7561 cgggcgatca ttctcaccgc acgcggcgcc gagcagcaca gctgcggaac cgacaccgtc
     7621 caggggttca tcaacctcgc cctggccctt ggactgcccg gcggcgaata ccaggggttc
     7681 gcatcgatca cgggacaggg taacggtcag ggcggtcgcg aacatggcca gaaatgcgac
     7741 cagctgccgg gctaccgcaa gcttgacgac ccggccgctc gtgctcatgt tgcgcaggtg
     7801 tggggaatca acccggccga cctgccgtcg tcgggccgtt cggcttacga aatgctggac
     7861 gccatcggca cccccggcgg tgtgcgggcg ctgtgggtaa tggcgtccaa catcgttgtg
     7921 tcgacgccga actcgctacg tgtcgccgcc aaggtcgcag atctggactt tctggtggtg
     7981 tccgacatct tcttgtccga gaccgccgcg ctcgccgacg tggtgttgcc gacggcccag
     8041 tgggccgagg agtcgggcac catgaccaac ctggaaggcc gggttatcct gcgccgcaaa
     8101 gcggttgcgg cccctggcga ggtccgcagc gacctggacg tgatggccga cctggcgaaa
     8161 cgcctagggg ttcaggggtt ttccgccgat cccgaagagg tgttcgacga gctcacccgg
     8221 gccagcgcgg gcggaaaggc cgactaccgg ggcatcagct atgcccgcat cgacgccgaa
     8281 cagggggtgt tctggccctg cccttccccg tcctacccgg ccaccgtacg catgttcgcg
     8341 caagacttcc cgacgccgga ccggcgggcg cacttccatc ccgtcgaaca ggccgcgccc
     8401 gccgagcggc ccgacgacga attcccgtat tacctcacca ccgggcgggt gctgcgccag
     8461 taccagtccg gcacgcagac gcggcgggtc gcttcgctcg ccgaagcgga gccggagccc
     8521 gtggtcgagc tgcatccgat gctggctcaa ctggttggtg tccgaaccgg cgagaaggtc
     8581 cggctgcgga cccgcagggg agaggtcgtg atgacggcgc gcaccgaaag cggcatccgc
     8641 ccggacaccg tgttcgcgcc gtttcactgg ggcggtgccg ggaacatcaa ccggctcacc
     8701 atcgcagagt tggacccaca ttcgcggatg ccgtcgttca aggtctgcgc ggtcgcgctc
     8761 agccgagccg acacagccga tacgaggaag agagccgacg atgggtgatg acagcacacc
     8821 gaaattcctg caaggagtgt tcacgttcga cggaaagggt tacgacgcac cgtttctgct
     8881 cgactccacg ctgcgctacg tggtgcccgc cggtgtgatc agccagccgc tgtacttccg
     8941 tggcggcaac agcgccgacg agctgatcac cgttatcgtg atgcgcgacg gcgcgccgat
     9001 gcgatacttc cccatcggcg ccaaagacgc tgtgcacgtg ccgcttcggg tcgtcgaaga
     9061 cttgatcgcc gacacagtcc tcgaggtgtt cgtcaccgca cccgacggac tgagcggcac
     9121 ggtcatcgtc gacatcggcc tcgtggaggt ctagccggtc atgacactgc ttgcgcaacc
     9181 ctcgacgaat gggcacgctc gcccggtccc ggcccgcctg gtgatggtcg gcaacgggat
     9241 ggccggggcg cgcgcgctcg aggagatcat cgcccgcggc ggccgcgaac ggtttgccat
     9301 tacggtcttc ggcgacgaac cgtacggcaa ctacaaccgg atcctgttgt ccaacgtgct
     9361 ggccggttcc gacgatgccc gcgagatcta cctcaacccc ctggactggt acaccgagaa
     9421 caacatcgac ctgcgtgccg gcgtccgcgt cgtgcgcatc gacccgttcg cgcatgtggt
     9481 gttcgccaac gacggcacca agatccgtta cgacaagctg atcctggcga ccggcagccg
     9541 atcgttcttc ccaccgatgg agggcatctg gcaggccgat aacaagaccc tgatccccgg
     9601 cgtgttcggg ttccgcagcc tcgacgactg cgaggcgatg atcgagttcg ccaaagggcg
     9661 ctcgcacggc ctcgtcgtcg ggggcgggct gctcggcctg gaagccgcgc gaggtctgca
     9721 gaatcggggc ctgcaggccg gcgtcgtcca gagcgggccc acgctgatga acgcgcaact
     9781 cgacgaccag ggcggcgcca tcctgcgcag actggtggag accgttggca ttgcggtgca
     9841 caccggcaaa cggcccacct ccgtgatcac cgagggcgcc ctgcccgtcg cgatgggatt
     9901 cgacgacggc acccggctgc cgtgcgacat ggtggtgctg gccgccggca tccggcccaa
     9961 cgtcggtctg gcccaacgcg ccggactcac cgtcgaaagg gcaatcgtcg tcgacgacca
    10021 gatgcgctcg atcgacgacg acgacatcta cgtcgttggt gagtgcgcac agcaccgcgg
    10081 ccaggtctac ggtctggtcg cgccgctttg ggagcaggcc ggcgtgctcg ccgatcatct
    10141 cgccggcacc aatccccgtg cggcatacca tggttcacgc accgcaacca agctcaaggt
    10201 cgccggcgtc gacgtggccg cgatgggcct gaagggcccg gagaacgccg acgacgaatt
    10261 cgtgcagtac gtcgagccca aacacggggt ttacaagacc ctcgtcgtcc gcgacgacaa
    10321 gctcgtgggc gcgacgctgg tcggtgacgt cagcaaggtg gcgttcctga tgcaggcctt
    10381 cgaccgcggg cttccgcttc cgccggaacg cgtttcgttg atgttcgaca tcggcacacc
    10441 cgacgtggcc gtcggcgcag ccgagctttc cgacgacgca caggtgtgca actgcaacgg
    10501 ggtcagcaag gccgcgatcg tcaactgcat caagcagggc cagacatcgc tgaccggcgt
    10561 gatgaacgcc acccgcgcag gcaagggctg cgggtcgtgc aagggcctgg tcggccagat
    10621 cgtcgaatgg gccgtttccg agtccggtgc cgaggtttgc gaggacgttg cgtccagctg
    10681 gtatgtgccg ggggtgccgt acgagaagcc ggagctgatg cggcttgtcc gcgagctgaa
    10741 cctgcactcg gtgtcgtcgg tgttcgaggc gctggcgccc gaaggccgcg aggacgccca
    10801 atcgaagatg gcgttgtcgt cgctgctgga gatgatgtgg ggcgacgaat tcgtcgacga
    10861 acgcgccagc cgcttcatca acgaccgcgt gcacgccaac atccagcgcg acggcacgtt
    10921 ctcggtggtg cctcagctca agggtggcgt gaccaccagc gaacagctgc gcaagatcgc
    10981 cgacgtcgcc gacaagtacg cgattccgat gatcaaactc accggcggcc aacgcatcga
    11041 cctgctcggc gtgcgcaagg aagacctccc cgcggtatgg gccgacctcg gtatgccgtc
    11101 gggctacgcc tacggcaaga gctttcgcac tgtgaaaacc tgtgtgggaa gcgacttctg
    11161 ccggtacggg ctgggcgact ccaccgcgtt gggcatcgcg atcgaagagc gctaccaggg
    11221 cctggcgagt ccggcaaaga tgaagctggc cgtcaccggc tgtccgcgca actgcgccga
    11281 ggcgctgtgc aaggacctgg gcgtggtcgc gatcgagggc gggcagtggc agatctacgt
    11341 cggcggcgcc gcgggcgcgc acgtgcgcaa gggcgacctg ctcgcaaccg tcgacagcca
    11401 tcacgaggtg atgacgctca ccggtcgctt tttgcagtac taccgggaga acgccaattg
    11461 gctcgagcgc acctacactt tcgtgcctcg gattggaatc gagcgcattc gcgcgatcgt
    11521 ggtcgacgac gccaacggca tcgccgccgc cctcgacgca cggatgcaga aatcggttga
    11581 ggcttaccgt gatccgtggg cccagagccg ggatgaaccc gaggacaacc aattccgtcc
    11641 ttcgctgccg ctgatcccgc ttccgcaggt gccggtgcga tgaccgacat cagcctgggc
    11701 cccgtcgacg accttcccgt gtgcgagggt cgcgccttcg ccgtcgaggg ccgccagatc
    11761 gccgtcttcc ggctgcggga cggctcactt cgcgcgctcg atgccgtctg tccgcaccgg
    11821 ggcggaccgt tggccgacgg cctgatcgac gatcgcgtcg tcgtctgccc gctgcacggc
    11881 cgcgcatacg aactcgacag cggatgcgag gtcgccggcg gcggcgcgtc ggtgaccacg
    11941 tacccggtac acgccgacag cggcgggacg ctgcgggtca gcctcgaacc ggccaacacc
    12001 gaatttcacg ggcgcaacgg agcgcattcg ccaaccggcc cgctccccag gaggtcacca
    12061 tgaccgcaca accggcaacc acggcacccg cggcaaccac ggcaacgccc gccccgccgc
    12121 tgtcgcggct gcgcgcgggt catcatcgga tctcgggatg ggatcccgag gacctgcaag
    12181 cgtggcaaaa cggcggaagc aaggtggcgc ggcgcaacct cgtctggtcg atcttcgccg
    12241 agcacatcgg cttttcggtg tggtccatct ggtcggtgat ggtgctgttc atgccgcaga
    12301 acgcctacca catcgacgcg gcgggcaagt tctacctggt agccgtcccg acgctggtgg
    12361 gtgccgtgct gcggctgccc tacaccaccg ccgtcgcgcg gttcggtggg cggaactgga
    12421 cgatcgtcag cgcgctggca ctgctggtcc cgttgacgct gacctggtgg ttcatggtgc
    12481 atccgggctt ctcctacacc acgtgcctga tcgtggcggc cttcgccgga ctcggcggtg
    12541 gcaacttcgc ctcgtcgatg acgaacatca acgccttcta cccggctcgc ctcaagggct
    12601 gggcgctcgg cctcaacgcc ggcgggggca acatcggagt gccggtgatc cagctggtcg
    12661 gcctgctggt catcgcaact gtgggcaacc gttcgccgca gatagtgtgc gctacctacc
    12721 tggttctggt cgggctggcc gggttgggcg ccgcgttgtt catggacaat ctggataacc
    12781 agaagtccaa tgccgcggcg atgctgcggg tgatccggtt cgccgacacg tggttgatct
    12841 cgttcctgta catcgggacc ttcggatcct tcatcggcta cagcttcgcg ttcgggcagg
    12901 tgttgcagat caactacctg tccgtgccgc acaccacggc ggcggcggcc gccctgcacg
    12961 cttcgcagat cgcgttcctg ggtccgctac tgggatcgct gagccggccg ttgggcgggc
    13021 ggctcgccga ccggatcggt ggcgccaaga tcaccatggc ctgcttcgtc gcgatggtgc
    13081 ttgcggccgg ggtcctggtc gtagccggga cccgcggcga cgcccacccc ggcaagatca
    13141 gccacggaac catggtggcc tacgtcgtcg gtttcatcct gttgtttctc atctccggca
    13201 tcggcaacgg atccgtctac aagatgatcc ctgccatctt cgcggcgaaa tcgcttgacc
    13261 tcaaaaatgt ttcggctgtg gaaacgctga actgggagcg gtcgatggcc ggcgcgttga
    13321 tcggcatcgc cggagcaatc ggcgctctgg gcggcgtcgg catcaacgtc gtgctacgcg
    13381 cttcctacgc tggggcccag aaatccgcga cgacggcctt ctgggtgttc ctggttttct
    13441 acatcgtgtg cgtcggggtc acctgggcgg cgtacgtgcg gccgcgcggg gccacgtcac
    13501 tcgtctgagt cccatccggg cgtggtttcg cgccgaatca ctctcgccag gactctgggt
    13561 tagccgaccc gggatccggg tatcgattcg attgatcgaa atgccggggt aactcgtgta
    13621 catcggcatg tcggcagcgc gggttggttg caacgttcgc caacgaggcg ggtccggccc
    13681 gcccggtcgg gcacgacacg agaggcgtga catgctgggc catctctacg atcgggcgct
    13741 cggcggcgag cggtgttgga tccgtcatga ggacggcgaa gtcctgcccc tgcccgccca
    13801 tcgttggttg ggtgctcgcg gcatttccga cggttcagac ccatccgctg acgcccttga
    13861 ggaggccttc gacgaggccg tcacgcagct gtgcaccgga ccgacgatcg agctgggatg
    13921 cgggcccggg cgtctggtgg cgcgcctgac ccggcgggga atacccgcgc tcggcatcga
    13981 tcggtcggcg accgctgttc gcctggcggg ttgcggcggc gcgccggccc tgctgggcga
    14041 cgtgttcgcg ccgctccccg ggacgggtct ttggcagacg gtcttgctgg tcgacggcaa
    14101 cgtcggcctc ggcggcgatc cccgacggat cctggcgcgt gccgccgagc tgctcggctg
    14161 cggtgggtgt tgcgtcgccg agttcgacgc cgaggtcatc gggatctgtc cgcgatgggt
    14221 gcggctggag tcggccggcg acgtcggccc ctggttccgg tgggcatcgg tcggtgtcga
    14281 cagtgccgcc acgctggccg cgcacgtggg cctgacgctc accggcgttc gtctgatcgc
    14341 cggccgggtg gttgcgatac tggccgccgc gtgagcaagc tggaccggca tccgccaccg
    14401 ggcgtgcccc ggcggacccg ggtgctcgtc cacttccgga gcccgttgcg cggtccgtgg
    14461 ctgacgtcgg tgttcgggct ggtgctgctg atcacgctgc cgatcgtcat cgttaccggg
    14521 ctgctgtcct acatcgccta tgcgccgcaa ctcggccagg ccatccccgg cgacgtcggc
    14581 tggctgcgtc tgcccgcctt cacgtggccg acccgcccgt cgtggttgta ccgcctgacc
    14641 cagggcctgc acgtggggct gggcctggcg atcatccccg tggtgctggc caagctgtgg
    14701 tcggtgattc cacggctgtt cgtgtggccg cccgcccgct ccatcgccca actgctggag
    14761 cggctgtcgt tgctgatgct ggtcggcggg gtgctgttcg aaatcgtcac cggggtgctc
    14821 aacatccagt acgactacgt cttcgggttc agcttctaca ccgcccacta cttcggggcg
    14881 tgggtgttca tcgccggctt tctgatgcat atctgcctca agattccacg catggtcacc
    14941 gggttgcggt cgctgccgct gcgagaagtg ttgcgcacca accgcagcga tactcgtccg
    15001 caatcacccg atcccgacgg cctggtggcc ggcgacccgg ccgaacctac gatgagcaga
    15061 cgaggtgcct tggcgctggt cggctcggga gtgctgctgg tttgcacgct gaccgtgggc
    15121 caaaccctcg gcggtgcggc gcgtggcgcc gccttactgc tgccgcgggg gcgtggccct
    15181 ggtggtctga ggggggactt cccggtcaac aagaccgccg ccgccgcggg gatcacgccc
    15241 gaaagtgtgg gcggcggttg gctgttgacc ctgcgcggcg gaccctcgga ggtggcgctg
    15301 gaccgcgcga cgctggccgc gctgccgcaa tacacggctc ggctgccgat cgcgtgcgtc
    15361 gaggggtggt cgacggtgca aacgtggagc ggggttcggc tggccgagct ggcccggatc
    15421 gcgggcgtgc ccgcgccacg cgccgcgcgc gtgacgtcgc tgcagcgggg cggcgcgttc
    15481 ggtgcggcga cgttgcaggc caatcagatt cgcgatccgg ccgcgctgct ggcgctgcgc
    15541 gtcaacggcg cggacctgtc cctggatcac ggctatccgg cacggatcat cgtcccggcg
    15601 ctgcccggcg tgcacaacac caaatgggtg gcgtccatcg agttcgagcc gagctgagat
    15661 gcccggattg gtaacgcgtt tcagagcctg ctacggatcg catccgttgc atctgctgac
    15721 catgctgtcc ggtttcgccc tgttgggcgc cgtcctgacc atcttcaagc cggcgacgct
    15781 gtggaatccc ggcgcctggt ggcagtcgat cgccgtgtgg ttggccgcct gcgtcgtcgc
    15841 ccacgacctg ctgctgtttc ccctctacgc gctggccgac cgggtgctgt ttacccggat
    15901 cggccggcga ccgtcccctg tccgtcatcc aaaagtgagc gcccgcaacc acattcgggt
    15961 gcccgctctg ggtgccgggc tgacgctgct gatattcctt ccggggatca tccaacaggg
    16021 cgccccgacc taccaggctg ccaccggaca gacccagcag ccgttcctgg gcaggtggct
    16081 gctgctcact gcggcaatgt tcgcggtgag cgcgctgtgc tacgtcgcgc gactcgccgt
    16141 ctgcgggcgg cgggcgcgcc gcggcgacga cctcgctgca agcgtcggtt ttggtgatcc
    16201 gcccgggagt acgctccgta catgacggcg aggggacccc gcgtgacgcc ccggatcaga
    16261 tggttgctgc gggccggccc cgccgactac atcctggcct tcagcatggc gggtgcttcg
    16321 ctgccggtgg ttggcaagcg cctcgagcca ctgggtggcc tgaccgccat gggcgtctgg
    16381 ggtgcccggc aggcgccaca ggccctatcg gcgatgacgc aggagtggct gacgccgggc
    16441 agtggcgacg tccgccggcg ggatcgcgag agcacccagg aggttgcccg cgccgcgctg
    16501 cgcggcgtcg tgtccgccgc ggacctcgac gtcgaatggc ccaccgccga gcggacaccg
    16561 ccgatctggg atgcgatgag tcagcgccgc tacctctacc gccgcggggt ccactacggc
    16621 aacagcaggg cccaggtgct cgacgtgtgg cgcctcaagg agctgcccgc gcagcccgcg
    16681 ccggtcctgg tcttcgtccc gggcggcgcc tgggttcacg gcaggtacct gggtcaggga
    16741 tccgcgctga tgtcgcggct ggccgagcaa gggtgggtgt gcctggcgat cgactaccgc
    16801 gtcgctccgc accaccgctg gccgcgccac atcaccgacg tcaagacggc cgtcgcgtgg
    16861 gcgcgagcca acgtcgacaa gttcggcggc gatcgcaatt tcgttgcggt ggcaggttgt
    16921 tcggccggcg ggcacctcgc cgcgctggcc gggctgaccc ccgacgaccc cgcgctgcgg
    16981 gcggaactgc ccgatggcgc cgacacttcg gtggatgccg tggtcgggat ttacggccgc
    17041 tacgactggc acgaccgctc cactcccgaa cgtgtccggt tcgtcgactt cctggagcgg
    17101 gtggtggtcc gacggaccat tgcccgtcac cccgaggtgt tccgcgacgc gtcgccgatc
    17161 gcgcgcgtgc accggaatgc gccgccgttc ttggtgattc acggcagcaa ggatggcgtc
    17221 atcccggtcg accaggctcg cagcttcgtc gagcggctgc gggcggtttc gcattcggtg
    17281 gtcggctacc tggagctgcc cggggcgggc cacggtttcg acctcatcga cggcgagcgg
    17341 gcaggcgcaa tggcccacgc gactgcgctg ttcctcaacg aggtctatcg caccaggacg
    17401 cggatcggcg cgaaagaggt tatttgagcc gccacagctg agtatggtcg ccctatgggc
    17461 aaggcgcagc gatgaagcgg ctgagcggct gggacgcggt actgctgtac agcgagacgc
    17521 cgaacgtgca catgcacacc atcaaagtcg ccgtgatcga actggctccg gataggcgtg
    17581 acttcgatgt cgacgcgttt cgccaagtca tcgggggccg gctgaacaag ctcgagccat
    17641 tcacctacca actcgtcgaa atcccgttca agttccacca tccgatgtgg cgggagcact
    17701 gcgagatcga cctcgactac cacatccgcc cgtggcggtt gccggccccc ggcggtcgcc
    17761 gcgagttgga cgacgcgatc gggcggatcg caagcacgcc gctggaccgc gagcgcccgc
    17821 tgtgggaaat gtatttcgtg gacggtctgg ccgacaaccg ggtcgcggtg gtcggcaaga
    17881 tccaccacgc gctcgccgac ggtgtggcct cggccaactt gatggcgcgc ggcatggacc
    17941 tggttcccac gcccgagagc ggtccgcagg tgaccgaccc ggctccttcc aagccgcagc
    18001 tggtcacgtc ggcgttcatg gaccacctgc gccacctggg gcgtatcccc gcgacgatcc
    18061 gttacacggc cgacgggatg ggccgggtgc ggagcagttc gcgcaggctg tcagcggagc
    18121 tgacccggcc cttcacgccg cccccgacct tcatgaatca cctgctcacc ccggagcgca
    18181 ggttcgccac cgcgacgctg gcgctctccg atgtcaagga aaccggcagg aagctcgggg
    18241 caacgatcaa cgacatggtg ctggccatgt ccgccggagc gctgcgcacc ctgcagctgc
    18301 ggtacgacgg cacggcccag ccgctgctgg cctcggttcc ggtgagcttc gacttctcgc
    18361 cggagcggat ctccggaaac cgcttcaccg gaatgctggt cgccctcccg accgattccg
    18421 acgacccgct ggaacgcgtc cggtgcagcc gcgagaacgc catctcggcg aaggagagcc
    18481 atcacctgat ggggccggaa ctggtcagcc gctgggcggc ctacatgccg cccgcgggca
    18541 cggaggcctt cttccgctgg gcgtcgggcc gggacggaca gaacaaaatc ctgaacctga
    18601 acatctcgaa cgtgccgggt ccgcgtgagc gtggccgggt gggcggcgcg ctggtcaccg
    18661 agatctattc ggtggggccg ttgaccgccg gcagcggcct gaacatcacc gtgtggagtt
    18721 atgtcgatca gctcaacatc tcggtgctcg cggatggcgc gaccgtcaag gatccgcacg
    18781 aggtcaccga ggccatggtt gctgacttca tcgaaatacg cagggccgca gggctttccg
    18841 atgagctgac ggtcgtcgag accgcgatgg ctcaggcttg attcgacgct ttaccgagct
    18901 ggcgggtgga gcggcccggt atcggagtaa cgggattccc tccgacattc cggccattcc
    18961 agtcggccgt taccatcggt cgaagagccg ccaccgaatg cccgaaggag ccgtgcagca
    19021 ccgtgagcac tgggaacgca gacgccagcg tgggtgaacc cgaagttctg gtcgaacaac
    19081 gggatcggat cctgatcatc accatcaacc ggcccaaggc caagaacgcg gtcaacggcg
    19141 cggtcagccg gggcctggcc gacgcggtgg atcggctcga cgacgatgcc ggcctgtcgg
    19201 tggggatcct gaccggcgcg ggcggctcgt tctgcgcggg catggatctc aaggcattca
    19261 cccgcggcga gaacgtcgtg gtcgcgggcc gcggcctggg cttcaccgaa cgaccgccgg
    19321 tcaagccact catcgccgcg gtggaaggtt tcgccctggc cggcggctgc gagctggcgt
    19381 tggccaccga cctgatcgtg gcgtcccggg actccgcctt cggcatcccc gaggtcaagc
    19441 gcgggctggt ggccgccggc gggggactgc tgcggctgcc cgatcgcatc ccgtacgcga
    19501 tcgccatgga gctggccctc accggcgacc agctgcccgc cgagcgtgcc cacgaactgg
    19561 ggttggtcaa cgtgttggcc gagcccggca aggtgctcga ggccgcgatt gcgctggccg
    19621 agaagatcac cgccaacggg ccgctcgcgg tggccgccac caagcggatc atcaccgagt
    19681 cccgtggctg gagcccggac accatgttca ccgaacagat aaagattttc gctccggtgt
    19741 tctcgtccaa cgacgccaaa gaaggtgcga tcgcgttcgc cgagaagcgt ccaccgcact
    19801 ggacgggaac ttaaccgctc gtcgaacacc tgggatcgtt gttcgacacg cttcaactgg
    19861 tcagatagcg ctgcacggtt ggtccgagcc aggcaaccaa atcgtcgatg gcagttgacg
    19921 cgagtggctc gatcttgagc tggtagcgcg cgaacgccag cccggccaga tgactggcca
    19981 ccagctcgac ccgcagttcg gcatcggcca cgccgaattc cgcggcgacc cgcttcgcga
    20041 tggggccggc gaggctgtcg tgcaacacct ttcgcgcctg ggggtgaatc gccgccgagc
    20101 gccacagcgt caggaatggc tcgaagaccg cgccttcctc ccagtgctcg aggaagcggc
    20161 gcaccagcct cgggccgatc tgctcggttc ccgcgtcgag aagcgtcgcc agctggtgca
    20221 gcgggtgctc ggggccggtg agggtggcgg cggtgaaaag cccttccttg ctgtcgaaga
    20281 agtagtagac catcgcgacg tccacccctg cgtcggcggc gatggcgcgc accgtggcgc
    20341 cctcgtatcc gtcgcgcatg aagcgttcgc gcgccgcgtc gatgatgcgc tgcctgttct
    20401 gctgaccggt gcgccaccgg cccgggcgct gcgcttctga catgggcgaa agcatacttc
    20461 aacaagcgtt gatctttatc cggcggcggg cgtaggctcg actttgttca acgatggttg
    20521 aagtaatgcc ggaaggggac gaagccatgg tgacgaacac gcgcgaacga cggctttggt
    20581 ggcgacacct gatgtccgtc ctgttgtttc cggtgacggt gacggtcgtc gtcccggccc
    20641 tgatcgtgat cccggccggc ctgcgagggc cgcagctggg agtcactgcg acggtcccac
    20701 gggtgatcgt cggtagcttg ctgatcgccg ccggtttggg cctgctgatc tggacggtgg
    20761 tgctgttcga ccgggtgggc gaggggacgc tcgggctggg caatgtgatg ggggagccgg
    20821 tccatctggt cgtgcggggg ccgtatcgac acgtgcggaa cccgatgatc accggcgtgc
    20881 tgtgcatcct gctcggggag gcggcgatta ccgcgtccgg ctcgctgttg ctctggttcg
    20941 cgatctttct cacgttccag gcgacggcca ttcggttctg ggaggagcct catctcgcga
    21001 ggcgctacgg cagcgagtat gtcgactacc ggcgcaacgt tccgcgctgg atcccgcgca
    21061 tctcggcctg gagcgccggt cacaccggca ctagtcgttg ttgagccggc ggccggtccg
    21121 gctgagttac actatggact ggatttgtaa cgggctggga tcgaggatgt tcgaggatga
    21181 gcatttcgct gctgttggag atggccgcgt cgagcaaccc ggatcgcacg gccatcgtct
    21241 ctgcggagcc ctccggcccc gtgcgattga cgacgcagca gctcagcgat ctggccgacg
    21301 ggggcgcggc tgtgatcgcg ggatccaacg ccaaacacgt ggcctacgtg ggcgccggcg
    21361 gcgcgatgct gccggtgctg atcttcgccg ccgcgcgcgc cgggctcgcc ttcaccccga
    21421 tcaattaccg gctgtccgac gatggcatcc aggcgctgat ccggcggctg cccgaacccc
    21481 tggtgatcgt cgacggccgc taccggatca cgctcgggga tgcgtcggac cgggtgatcg
    21541 agtccgatga cttcctggcc gccgcccgca acgccgaccc gtcggactac gcggtggaat
    21601 tcccggaccc ggagtcggtc gcgatcgtgc tgttcacctc cgggacgacg tcggagccca
    21661 aggccgtaga actcacacac aacaacctga ccagctacgt caccggaacg gtcgaattcg
    21721 aatcggccga ccccgccgac gccgcgctga tctgcgtgcc gccgtatcac atcgccggag
    21781 tcggtgccgc gctgtccaat ctctacgccg ggcgcaagat ggtctacctg cccaccttcg
    21841 atcccttgga atgggtgcgg ctgatcaacg ccgaacacgt caccaccgcg acggtggtgc
    21901 ccaccatgct ggagcggatc gtgaccgtgc tcgaggccgg cgggcatcgg ctgccgtcgc
    21961 tgcgcaatct cgcttacggc ggctcgaagg tgggcctgcc gctggttcgc cgggctctcg
    22021 ggctgctgcc gaacgtgggt ttcgtcaacg cctacggctt gaccgagacc agctcgacga
    22081 tcgcggtgct gaccccggac gaccaccggg cggcgcaagc ggcgctttca tcgtccgaca
    22141 tcgcactggc caggcggctg ggatcggtcg ggcagccggt accggggatc gaattgcaga
    22201 tccgcggcga cgacggcacc gtgctcggcg cgggccagac gggagagttg ttcgtgcgcg
    22261 gcgagcaggt ctccgggcga tacacgggcg tcggctcggt gctcgacgaa aacggctggt
    22321 ttcccacgaa ggacatcgcc atgctcgacg aggagggcta cctgttcatc ggcgggcgca
    22381 gcgacgacac catcatccgc ggcggcgaga acatcgcacc tgccgagctg gaggaggtgc
    22441 tgatcgaaca tcctcacgtg cgcgacgtcg cggtggtcgg tgtcgaggat ccgcagtggg
    22501 gccaggcgat cgtcgccgtg gtggtgcccg ccgcgggtgt cgaacccgac gccgaggagt
    22561 tgcgcgagta tgtccgcggg agtttgcggg ggtcgcgcac tcccgaccga gtagtgtttc
    22621 gcggcgagct gccgacgacc gccaccggga aggtgcttcg ccgcgagatc atcgagacgc
    22681 ttcaagagga tgcgaaaagg acaagtgcat gatcaagaac ggaacccgtc tcgccagcca
    22741 ggtgtgtgac acgcaggtca tcgtcgtcag aagcgccgag agcctcgacg acttgcgttg
    22801 cggcggtgcg ccgatggtgg cgatcggcgc cgagcggtcg ggctcccttg acccggcatt
    22861 tgctggcggc accgtgatgg gcaagcgcta tgtcgtcgaa accggcgccg aggtgctggt
    22921 gaccaagccc ggcgccggaa gcctgagcgt cggtgcgacc gcgctgcagc tcaaagaggt
    22981 caagccgctg ccggccagcg actgaactcg gctactggcc cgtttgcccg tcgggtccgg
    23041 gaaccccgag caacaacccg ccggcgccgc cttgtccgac ggtgctgccc gcgccgaagc
    23101 tgtcccctcc ggcgccgccg ttgccgatca gctgggcgtt gccgccatca ccaccgttac
    23161 ccggttgcgg accggtgccc gcgctgccgc cggcgccgcc gttgccgatc agcagggcgc
    23221 cgttgccgcc gtgaccgccg gtggcgccgg tgatggccgc gttggtctgg ccagctccgc
    23281 cgccgtcgcc gccgttgccg aacagcccaa tggcattgcc gccggcaccg ccggcgccac
    23341 ccctcccgag ggtggattgc gcgccattgc cgccgtcacc gccgtcgccc cacaggatcc
    23401 cgccgttgcc gcccggaccg ccggggccgc cgaagccggc actgctgagg ccggtgccgt
    23461 tgccgccgcg gccgccaccg ccgatcaggc ccgcattccc gccggtgcca ccgctctgca
    23521 cgcccgagcc gttgccgcca tcgccgccgt tgccgctcag cagcccgccg acaccgccgc
    23581 cacccccgac ggagccgaag gagccgtccc cggcattgcc gccgctgccg ccgttgccgt
    23641 acaacaggcc gccgtggccg ccgtgaccgc ctgcacctcc gatgccacct gcaaaaactt
    23701 gggggacgtt cccgaggccg cccaccccac cggcgccgcc ggtgccgaac aagccgatgg
    23761 cgtcaccgcc ggcgccgccg agtccggcgg gaccgaacgc ggtgctgttg ttgtacgacc
    23821 cgcccgcgcc gcccgcgccg ccgttgccga acaccaggcc gccggcgcca ccggcgccac
    23881 cgttcccggc ggccacgccg gcggtggtgc tcagcccgcc cgcgcctcct gtgccgccgt
    23941 ggccgaacag accggccgcc ccgccggcac cgcccgggga gttcgccgcg cccgagccgc
    24001 cgttgccacc gttgccccac agaattccgc cggcctggcc gttctggccg gtgcccggta
    24061 aaccgttggt gccgttgccg atcaacggac gctgcaacag cagttcggtc ggcgcgttga
    24121 tggcgttgag cacatcttgc tgcagggtct gccatggatt ggctgccgcc
//
LOCUS       DASSGQ010000037         9087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_822936,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000037 DASSGQ010000000
VERSION     DASSGQ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9087
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822936"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="VFI55_01750"
     CDS             complement(<1..1475)
                     /locus_tag="VFI55_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HET7072792.1"
                     /translation="MTSLADRAAQMSPKAREVLARELVRAGTAFPTEVAEPIAVIGMG
                     CRLPGNVTGPESFWQLLLDGRDAVGEVPGDRWDADAFYDPDPQASGRMTTKWGGFVDD
                     VAGFDADYFGITPREAAAMDPQQRMLLEVAWEALEHAGLPSDSLAGKRIAAIMGLSSW
                     DYTIVNIERRAEIDAYLSTGNPHSAAVGRISYLLGLRGPAVAVDTACSSSLVAVHLAC
                     QSLRLRESEVALAGGVQLALSPFTGIALSKWSALSPTGRCKTFDATADGFVRGEGCGV
                     VVLKRLADAQRDGDRVLAVIRGSAINQDGRSNGMTAPNASAQRDAITTALRQADITAE
                     SVRYVETHGTGTILGDPIEFEALAATYGRGEGSCALGAVKTNLGHLEAAAGITGFIKA
                     TLAVGRGYIPRNLHFSRWNPAIDASETRLYVPAEGASWPAGAARRAAVSSFGLSGTNA
                     HVVIEQAPDVVVGAGAGSVSTSVVSTLVVSGKSDRRVAATAG"
     gene            complement(1472..7996)
                     /locus_tag="VFI55_01755"
     CDS             complement(1472..7996)
                     /locus_tag="VFI55_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020786540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HET7072793.1"
                     /translation="MTTPSAPSHDRRAIITEALHKIDDLTARLEIAEKASTEPIAVVG
                     MGCRFPGGVNSPEQYWELLRDGRSGVVRVPSERWDADAFFTEDHTVPGTICNREGGFL
                     TGWQPDEFDAEFFSISPREAAAMDPQQRLLLEVAWEALEHAGVAPETIRGTQTAVFVG
                     VTAYDYMLTLSGQLRPEDLDAYIPTGNAANFAAGRLAYFLGVRGPAVVIDTACSSSLV
                     AIHLACQSLRSRESDTVLVGGTNLLLSPGPSIACSRWGMLSPEGQCKTFDAAADGYVR
                     SEGCGVVVLKRLADAQRDGNRILAVVRGSAVNQDGASSGVTVPNGPAQQALLRQALTS
                     ARLKPADIDYVEAHGTGTPLGDPIELDSLSKVFADRQDRGPLVLGSVKTNLGHLEAAA
                     GIAGFMKTVLSVGHGHIPRHLNFNQLTSHVGEGVSRLSIAAEAMQWPATANPRRAGVS
                     SFGVSGTNAHVVIEQAPDVVVGAGAGSVSTSVVSTLVVSGKSDRRVAATAGVLADWLA
                     GPGASIPLADVAHTLNHHRARQHRFATVVARDHAQAVAGLRALASGAVAPNQAAGGGQ
                     GTVFVYSGQGSQWAGMGRQLLADEPAFAAAIAELEPDFLAQTGFSLHDTLAQGRELLG
                     IHTIQPVLVGLQLALTALWRSYGVQPDAVIGHSMGEVSAAVVAGALTPAQGFQVIATR
                     SKLMATLSGQGAMALLELDAQATAALIADYPQLTLAVHASPRQSVIAGPPDQVDAIIT
                     TVSAENLLARRIEVDVASHHPIIDPILAPLHAALADLTPHPPTIPLITTTHDTHDTHD
                     THDAPLLDADYWTANLRNPVRFHQAITTAGAHHHTFIEISPHPLLTHAITDTLQHAHT
                     LPTLQRDTDDTITFHTNLNATHTTRPPDTPHPSEPHPLLPTTPWQHTRHWISATSKAS
                     HDANAHPLLGTGVIDPTNGTRVWQTKLGPDLLWLGDHCVDDVCVLPGAAYAEIAVAAA
                     NEAFGAFGTRADRSWMIRELSLEQLMHIADDTVLVTTLTGDEQLCRVEMRTRSATSGW
                     IKHAAATVTPASHAAPMDSTPRPADEVVTEMQADDLYERLRAAGQQHGPAFQGITGLT
                     VAPSGAATAQVRLPAKAKAGSRKFLMHPVMMDVALQTLGATGLASELAAGAGDQRATV
                     LPVRFAGIHVYGDISDGARAIATLATTDSPDRLVGHVLLTDAEDRPLLEIDEVDMAVL
                     RSRGGAAELGNRLFTLDWEPAALEKSAGPAGAAGAVLLIGDPDDADPLLAGLESALRE
                     RTTHCELVARDDDQSIRAAITTSDTEWAGIVVVCPPRAVDELQSVPTQLELARRRTLQ
                     LAEIVKTVSRMGARNSPRLWVITRGARQLHPAEPVTLAQTQLRGLARVLTFEHPELKT
                     SIVDIDPDGSEPLSDLVDELLTGADQDEVALRGGRRYVSRLVAAPVTPSGELAIESRE
                     TTVDLDTGAVRLELDHVGRLDGLKVHAVKRIRPTGHQVEVRITAAALNFSDVLKVMGV
                     YPGLGGNAPIIGGECAGTVTAIGSPLESGLEIGQRVIAFGAGTIGSHLTTSQDLVVPI
                     PDALSDSEAAAFGVAYLTAWHSLCEVGRLGPGERVLIHSATGGVGLAAMSIAKMIGAR
                     IYATAGTDAKRDMLSGMGVEYVGDSRGVTFADEILDLTDGAGVDIVLNSLPGEAIRAG
                     MRVLAPGGRFIELGKKDVFADASVGLAALARSGTFAVVDVDLNMRLQPKRYREYLQRI
                     LRHAAAGELTPLPVTEFAFADAIDAFRLMASGGHTGKIVIAIPQSGSITALAPRPAQP
                     LVSPDGGYLVVGGMGGLGLVTARWLAQQGAGLVVVNGRSAPGAEVLAEIDAIRADGGR
                     VEVVTGDIADPETARRLVAAVEDGDFRLAGVVHSAMVLADETVLNISESAATRVFGPK
                     VAGGWWLHEATAHAELDWWLTYSSAASLIGSPGQGTYAAANSWIDGLVAYRRSLGLPA
                     AGINWGPWAEVGRAQFFADLGISMISSAEGLAAVQLVLTADRARTGVFSLDARQWFQS
                     FPAAAGSSLFAKLQDSIRVERRGGGRIRVELEACDPAERPGRLADAIADEIRAVLRST
                     EPIAHDRPLESFGLDSLMALELRNRLESSLGTTLPVALVWAYPTITDLAGAMCERLGY
                     TVPAEDQQPPDEEIELSDEEMDLLSDLVAASELEAATGGVEQ"
     gene            complement(8002..>9087)
                     /locus_tag="VFI55_01760"
     CDS             complement(8002..>9087)
                     /locus_tag="VFI55_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl reductase"
                     /protein_id="HET7072794.1"
                     /translation="LVQMGARTIVAVSRNPGARLAELAGSLEPAGVRLVQIAADASDP
                     AAMSALFDRFGEDLPPLEGIYLAAFAGAPVLLSEMTNGDVAAMFRPKLDAARLLHRLS
                     LKKPVRQFVLFSSISGLIGSRWLAHYTATSAFLDALAHARRLVGLPATVVAWGLWQSL
                     ADAQQDASQVSSESGLQPMADEVAIGALARAMSPDAGPHSVVVAADWPLLAAAYRTRG
                     SLRMVDDLLPAPDGQTMPESEFRKALRKCQPERRHDMLHDQVSMLASRVMGLPDTQTL
                     DPSAGFFQLGMDSLMSLTLQRAVSEALGEFLPASVVFDYPTVYGLTDYLATVLPELIE
                     TAEQPAADVYDDLTEDELLQQLSQRLS"
BASE COUNT         1399 a         3085 c         3162 g         1441 t
ORIGIN      
        1 cccgcggtgg ccgccacccg ccgatccgac ttacccgaca ccaccaacgt cgacaccacc
       61 gacgtcgaca ccgacccagc acccgccccc accaccacat ccggcgcctg ctcaatcacc
      121 acatgcgcat tcgtcccgct caggccgaag gacgacaccg ccgcgcggcg cgccgccccg
      181 gcgggccacg acgcgccctc cgccggcacg tagagccgcg tctcggatgc gtcgatggcc
      241 ggattccagc gggagaagtg cagattgcgc gggatgtagc cgcgtccgac ggccagcgtc
      301 gccttgatga acccggtgat cccggccgcg gcctccagat gaccgagatt ggtcttgacc
      361 gccccgagcg cgcacgaacc ctcgccgcgg ccgtaggtgg cggccagcgc ttcgaactcg
      421 atggggtcac ccaaaatggt tccggtgcca tgggtttcga cataccgcac gctctccgcg
      481 gtgatatccg cctggcgcag tgcggtggtg atcgcgtcgc gttgagccga tgcgttgggg
      541 gcggtcatgc cgttggaacg gccgtcctgg ttgatcgccg aaccacggat gaccgcgagc
      601 acccggtcgc catcgcgctg cgcgtcggcg agccgcttga gcaccaccac cccgcagccc
      661 tcgccgcgca cgaagccgtc ggcggtggcg tcgaacgtct tgcaccgacc ggtcggcgac
      721 agcgccgacc acttggacaa cgcgatgccg gtgaacggcg acaaggcgag ctgcacgccg
      781 ccggccagcg ccacttcgct ttcccgcagc cgcaagctct ggcaggccag gtgtacggcc
      841 accagcgacg acgaacacgc ggtgtccacc gcgacggccg gaccacgcag gcccagcaga
      901 taggagatcc ggcccaccgc cgcgctgtgt ggattgccgg tgctcaggta cgcgtcgatc
      961 tcggcacgac gctcgatgtt gacgatggtg taatcccagg acgacagccc catgatcgcc
     1021 gcgatgcgct tgccggccag cgaatccgag gggaggccgg cgtgttcgag cgcctcccag
     1081 gcgacttcga gcagcatccg ctgctgggga tccatcgcgg cggcttcccg tggggtgatg
     1141 ccgaagtagt cggcgtcgaa cccggcgacg tcgtcgacga atccgcccca tttggttgtc
     1201 atccgcccgc tcgcctgggg atccgggtcg tagaacgcgt ccgcgtccca ccggtcgccg
     1261 ggcacctcgc cgacggcgtc gcggccgtcg agcagcaact gccaaaagct ttccgggcca
     1321 gtgacgttgc cgggcaatcg gcaacccatg ccgatgaccg cgatcggttc ggccacctcg
     1381 gtggggaacg ccgttcccgc ccggaccagt tcacgcgcga ggacctcgcg cgccttcggc
     1441 gacatttgcg ccgcgcgatc ggccagactt gtcattgttc gacgcccccc gtcgcggcct
     1501 ccagctcgct cgccgcgacc agatccgaca acagatccat ctcctcgtcc gacagctcga
     1561 tttcctcatc gggcggctgc tggtcctcgg ccggcaccgt gtagccgagc cgttcgcaca
     1621 tcgcgccggc cagatcggtg atggtcgggt acgcccacac caacgccacc ggaagcgttg
     1681 tgcccaggct tgattcgagc cggttgcgca gttcgagggc catcagcgag tccaggccga
     1741 acgactccag cggccggtcg tgtgcgatcg gctcggtgga gcgcagcacc gcccggatct
     1801 cgtcggcgat cgcgtcggcg aggcgtccgg ggcgctcggc cggatcgcag gcctccagct
     1861 ccacccggat gcggccgccg ccgcggcgtt cgaccctgat cgagtcctgg agcttggcga
     1921 acagcgacga gcccgccgcc gcggggaagg actggaacca ctgccgcgcg tcgaggctga
     1981 acacaccggt gcgggcccgg tcggccgtca acacgagctg caccgcggcc agcccttcgg
     2041 cggagctgat catcgagatg ccgagatcgg cgaagaactg ggcacgcccc acctcggccc
     2101 agggacccca gttgatcccg gccgcaggca aacccagtga ccgccggtag gcgaccaggc
     2161 cgtcgatcca cgagttcgcg gcggcgtagg tgccctgtcc cggcgatccg atcagtgacg
     2221 ccgccgacga atacgtcagc caccagtcga gttccgcgtg ggcggtggcc tcatgcagcc
     2281 accagccgcc ggcgaccttc gggccgaaca cccgcgttgc ggccgattcg gagatgttca
     2341 acacggtttc gtcggccagc accatcgcgc tgtgcacgac gccggccagc cggaaatcgc
     2401 cgtcctcgac ggcggcgacc agccgccgcg cggtttcagg gtccgcgatg tcgccggtga
     2461 ccacctcgac ccggccaccg tcggccctga tcgcatcgat ttccgcgagc acttcggcgc
     2521 cgggggccga acgtccgttc accacaacca atcccgcgcc ctgctgggcc agccagcgcg
     2581 cggtgacaag gcccagacca cccataccgc ccaccaccag gtacccgccg tcgggactga
     2641 ccaggggctg ggccggccgc ggagccagcg cggtgatgct gccgctctgc ggtatcgcga
     2701 tgacaatctt gccggtgtgg ccgccggatg ccatcagccg gaacgcgtcg atcgcgtccg
     2761 cgaacgcgaa ctcggtgaca ggcagcggcg tcaactcgcc cgcggcggcg tgccgcaaga
     2821 tccgttgcag gtattcccgg tatcgcttcg gctgtaggcg catattcagg tcgacgtcga
     2881 ccaccgcgaa ggtaccgctg cgggccagcg cggccaatcc gaccgacgcg tcggcgaaga
     2941 cgtccttctt gccgagctcg atgaaccgtc cgcccggggc cagtacccgc atgcccgcgc
     3001 gaatggcctc tcccggaagg gaattgagga cgatatcgac tccggcaccg tcggtcaggt
     3061 caagaatctc gtcggcgaat gtcacgccgc gtgagtcgcc gacgtattcg acgcccatcc
     3121 cggacagcat gtcgcgcttg gcgtcggtgc ccgcggtggc atagatgcgc gcgccgatca
     3181 tcttggcgat cgacatcgcg gccaaaccca caccgccggt ggcggaatgg atcaatacgc
     3241 gctcacccgg ccccagccgg ccgacctcgc acaatgagtg ccacgcggtc aggtaggcca
     3301 cgccgaacgc ggcggcttcg gaatccgaga gcgcgtcggg aatcggaacg accaggtcct
     3361 gactcgtggt gaggtgagac ccgatggtcc cggcgccgaa agcgatgacc cgctgcccga
     3421 tttcgagtcc ggattcgagg gggctgccaa tggcggtcac cgttccggca cactcgccgc
     3481 cgatgatcgg cgcgttgccc ccaaggcccg ggtagacgcc catgaccttc aggacatcgc
     3541 tgaagttcaa cgcggcggcg gtgatgcgga cctcaacctg gtggccggtg ggccggatgc
     3601 gcttgaccgc gtgaactttc agcccgtcga ggcggcccac gtggtccagc tccagcctga
     3661 ccgcacccgt gtcgaggtcc accgtggttt cccggctctc gatggcgagt tcgccggacg
     3721 gggtgaccgg tgcggccacc agccggctca cgtaccgccg accgccgcgc agcgccacct
     3781 cgtcctggtc ggcgccggtg agaagctcgt cgacgaggtc cgaaagcggt tcgctgccat
     3841 ccgggtcgat gtcgacgatc gacgtcttca actccgggtg ctcgaaggtc agcacccgtg
     3901 cgagtccgcg cagctgtgtc tgggcgaggg ttaccggctc ggcggggtgc agttgtcgcg
     3961 cgccgcgcgt gatgacccac agccgcgggc tgtttcgggc gcccatgcgc gagactgtct
     4021 tgacgatctc ggcgagctgt agggtgcgcc gccgcgccag ctccaattgc gttggcaccg
     4081 actgcaattc atcgaccgcg cgcggtgggc agacgacgac gatgccggcc cattcggtgt
     4141 cgcttgtggt gatcgccgcg cggatcgatt gatcatcgtc gcgtgcgacc agctcacagt
     4201 gcgtggtcct ttcccgtagc gccgactcca acccggccag taacgggtct gcgtcgtcgg
     4261 ggtcgccgat cagcagcacg gcacctgccg cgccggcggg cccggccgac ttctccaggg
     4321 cggcaggctc ccagtcaagg gtgaacaggc ggttgccgag ttctgccgcg ccgccgcgtg
     4381 atcgcagcac cgccatgtcg acttcgtcga tctccagtag cggccggtct tcggcatcgg
     4441 tcagcagcac gtggcccacc agcctgtcgg ggctgtccgt ggtggccagc gtggcgatcg
     4501 cccgcgcccc gtcgctgatg tcgccgtaca cgtgaatgcc cgcgaatcgg accgggagca
     4561 ccgtggcgcg ctgatcgcct gcccccgctg ccaactcgct cgccagcccc gttgccccca
     4621 gggtctgcag cgcgacgtcc atcatgaccg ggtgcatcag gaacttgcgg gaacccgcct
     4681 tggccttggc gggcagccgc acttgtgcgg tggccgcgcc ggagggtgca acggtgagcc
     4741 cggtgattcc ctggaaggcg ggaccgtgct gttgtcccgc ggcgcgcagt cgttcgtaca
     4801 ggtcgtcggc ctgcatttcg gtgacgactt cgtcggccgg ccgtggagtg ctgtccatcg
     4861 gcgcggcgtg cgatgccggt gtcacggtcg ccgccgcgtg tttgatccac cccgaagttg
     4921 cgctgcgagt gcgcatttca acgcggcaca gctgctcgtc gccggtcagt gtggtgacca
     4981 gaaccgtgtc gtcggcgatg tgcatcaatt gctcgaggga cagctcacga atcatccagg
     5041 accgatcggc ccgggtcccg aaggccccga aggcctcgtt cgctgcggcg accgcgattt
     5101 cggcgtacgc cgctcccggc aacacgcaga cgtcgtcgac gcagtggtcg cccagccaca
     5161 gcaggtcggg gccgagcttg gtttgccaca cccgggttcc gttggtcgga tcgatgacgc
     5221 ccgtgccgag caggggatgg gcgttggcgt cgtgcgatgc cttcgacgtg gcgctgatcc
     5281 agtgccgagt gtgctgccac ggtgtggtcg ggagcagcgg gtgcggctcg gaggggtgcg
     5341 gggtgtcggg tgggcgcgtg gtgtgggttg cgttgaggtt ggtgtggaag gtgatggtgt
     5401 cgtcggtgtc gcgttggagg gtgggcaggg tgtgggcgtg ttggagggtg tcggtgatgg
     5461 cgtgggtgag cagggggtgg gggctgattt cgatgaaggt gtggtggtgg gcgccggcgg
     5521 tggtgatggc ttggtggaag cgcacggggt tgcgcaggtt ggcggtccag tagtcggcgt
     5581 ccagtagtgg tgcgtcgtgg gtgtcgtggg tgtcgtgggt gtcgtgggtg gtggtgatca
     5641 gtgggatggt gggtgggtgt ggcgtcaggt cggccagcgc tgcgtgtagg ggggccagga
     5701 tggggtcgat gatggggtgg tgggaggcga cgtcgacctc gatgcggcgg gccagcaggt
     5761 tctcggcgct gacggtggtg atgatggcgt cgacctggtc ggggggcccg gcgatcaccg
     5821 attggcgtgg ggaggcgtgc acggccaggg tcagttgcgg gtagtcggcg atcagggctg
     5881 cggtggcttg tgcgtcgagt tcgagcaggg ccatcgcgcc ctggccggac agggtggcca
     5941 tcagcttgga gcgggtggcg atcacctgga atccttgggc gggggtcagt gcgccggcga
     6001 ccacggcggc gctcacttca cccatggagt gcccgatcac cgcatccggc tgcacgccgt
     6061 aggagcgcca cagcgcggtc agtgccagtt gcaggccgac cagcaccggt tggatggtgt
     6121 ggatgccgag cagttcgcgg ccctgggcca gggtgtcgtg cagcgaaaaa ccggtctggg
     6181 cgaggaagtc gggttctagt tcggcgatcg cggcggcgaa agcgggttcg tcggcaagca
     6241 gttggcggcc catgccggcc cattgcgagc cctgaccgga gtagacgaac acggtgccct
     6301 gcccgccgcc ggcggcctgg ttgggggcta ccgcgccgct ggccagcgcc cgtaaccccg
     6361 ccaccgcctg ggcgtggtcg cgggccacca cggtggcgaa ccgatgctga cgggcccggt
     6421 gatggttgag cgtgtgggcc acatcggcca gcgggatgct cgcgcccggc ccggccagcc
     6481 agtcggccag cacccccgcg gtggccgcca cccgccgatc cgacttaccc gacaccacca
     6541 acgtcgacac caccgacgtc gacaccgacc cagcacccgc ccccaccacc acatccggcg
     6601 cctgctcaat caccacatgc gcattcgtcc cactgacacc gaacgaggac actccggcgc
     6661 gacgcggatt tgccgtggcc ggccactgca tcgcctcggc cgcgatgctc agccgcgaaa
     6721 cgccttcgcc gacatgcgaa gtgagctgat tgaaattcag gtgccgcgga atgtgaccgt
     6781 gcccgacgga gagcacggtc ttcatgaacc cggcgatccc ggccgcggcc tccagatgac
     6841 cgaggttggt tttcaccgat ccgagcacca gcggcccgcg gtcctgccgg tcggcgaaca
     6901 ccttactgag agagtcgagt tcgatcgggt cgccgagcgg cgtgcccgtc ccgtgcgcct
     6961 cgacgtagtc gatatcggca ggcttcagtc gcgccgacgt cagcgcctgg cgaagcaggg
     7021 cctgctgcgc cgggccgttg ggcaccgtca ccccgctgct cgcgccgtcc tggttgaccg
     7081 ccgaaccacg caccacggcc aggatccgat tgccgtcgcg ctgcgcgtcg gccaaccgct
     7141 tgagcaccac cacgccacac ccctcgctgc gcacatatcc gtcagcggcg gcgtcgaacg
     7201 tcttgcactg gccctccggc gacagcatgc cccaccgcga gcaggcgatg ctgggccccg
     7261 ggctgagcaa caggttggtc ccgccgacca acacggtgtc gctttcccgc gagcgcagac
     7321 tctggcaggc caggtgtatc gccaccaacg acgacgagca ggcggtatcg atgaccacgg
     7381 cggggccgcg taccccgagg aaataggcca accgaccggc ggcgaaattc gcggcgttgc
     7441 cggtcggtat gtaggcgtcg agatcttcgg gccgcaactg gccggacagc gtgagcatgt
     7501 agtcataggc ggtgacaccg acgaacaccg cggtctgcgt cccgcgaatg gtctccggcg
     7561 ccacaccggc gtgctcgagt gcctcccaag cgacttcgag cagcaaccgc tgttgcgggt
     7621 ccatggcggc ggcttcgcgc ggtgagatcg agaagaactc ggcgtcgaac tcgtcgggct
     7681 gccagccggt gagaaatccg ccttcgcggt tgcagatcgt gcccggcacg gtgtggtctt
     7741 cggtgaagaa ggcgtccgca tcccagcgtt cggacgggac gcgaacgaca ccgcttcggc
     7801 cgtcccgcaa caactcccag tactgctcgg ggctgttcac cccgccggga aaccggcacc
     7861 ccatcccgac caccgcgatg ggttctgtgc ttgccttctc ggcgatctct agccgcgcgg
     7921 tgagatcgtc gatcttgtgc agcgcttcgg tgatgatcgc gcggcgatca tgcgacggag
     7981 cagacggagt agtcatggcg cctagctcag tcgctgcgaa agttgttgca acagttcgtc
     8041 ctcggtcaga tcgtcgtaga catcggcggc cggctgctct gccgtttcga tgagttcggg
     8101 tagcacggtg gccagatagt cggtgagccc gtacacggtc gggtagtcga acaccacaga
     8161 cgcgggcagg aattcgccga gcgcctccga gacggcgcgc tgaagtgtca ggctcatcag
     8221 cgagtccatg ccgagctgga aaaagccggc ggacgggtcg agtgtctgcg tgtccggtag
     8281 tcccatcacg cgggaggcca gcatgctgac ctggtcgtgc aacatgtcgt gccgccgctc
     8341 gggctgacac ttacgcagag ctttgcggaa ctcgctttcc ggcattgtct gcccatccgg
     8401 tgccgggagc aggtcgtcga ccatgcgcag cgacccgcgt gtgcggtatg cggccgccaa
     8461 caggggccaa tccgcggcca ccacaacgga atgcggcccc gcgtcggggc tcatggcccg
     8521 cgccagcgcg ccaatggcga cttcgtcggc cattggctgt agtccggatt ctgaactcac
     8581 ctggctggcg tcttgttgcg catcggccag cgactgccat aggccccagg cgacgacggt
     8641 ggccggcagc cccacgaggc gacgggcgtg cgccaacgcg tccaggaagg cgctggtggc
     8701 ggtgtagtgg gccagccacc gggaaccgat gagacccgag atcgaggaga acaaaacgaa
     8761 ttggcgcacc ggctttttca gcgacagcct gtgcaacagt cgggcggcgt ccagctttgg
     8821 ccggaacatg gcggcgacgt cgccgttggt catctcgctg agcaacaccg gtgcgccggc
     8881 aaaggccgcc aggtagatgc cctccagcgg cggcaggtcc tccccgaagc ggtcgaacag
     8941 ggcggacatc gcggccgggt cgctggcgtc cgcggcgatt tggaccagac gcacgccggc
     9001 gggctcgagg ctgccggcca gctcggccag gcgcgcgccc ggattgcggg acaccgcgac
     9061 gatggtccgg gcgcccatct gcaccag
//
LOCUS       DASSGQ010000038         7590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_33238, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000038 DASSGQ010000000
VERSION     DASSGQ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7590
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33238"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VFI55_01765"
     CDS             complement(<1..854)
                     /locus_tag="VFI55_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET7072795.1"
                     /translation="MFVTGGSIVYSASDLAAAARCEYALLREFDAKLGRGPAISTDRT
                     EDELLARTATLGTEHERRRLDQLRDEFGDGVAVIGRPAYTIAGLTAAAEATQRAIAGR
                     APVVYQAAMFDGRFVGFADFLILDGERYRVADTKLARTAKVTALLQLAAYADALSSSG
                     VEVAPEAELELGDGTVVRYRIDDLIPVYRAQRTLLQRLLDRHYAAGSPARWDDDAILA
                     CFRCPLCAEQLRATDDLLLVAGLRVAQRDKLIAAGITTITELADHNGPVPDLASAALA
                     KLTAQAKL"
     gene            complement(935..1480)
                     /locus_tag="VFI55_01770"
     CDS             complement(935..1480)
                     /locus_tag="VFI55_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppP/LprE family lipoprotein"
                     /protein_id="HET7072796.1"
                     /translation="MAAVILAAAMLSGCGSGDSTVAKTPQATTTAGAGAPSSAAPGTA
                     AGPSSSAAPADPCAINLASPTIAKVVSELPRDPRSQQPWSPEPMAGNYNECAQLSAVI
                     IKANTNADNPTTRAVLFHLGQFIPQGVPDTYGFNGIDPAQTTGDTVALTYPGGINGLN
                     TDVRFHWNGNGVELIGNTPGH"
     gene            1746..3476
                     /locus_tag="VFI55_01775"
     CDS             1746..3476
                     /locus_tag="VFI55_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7072797.1"
                     /translation="MNLSDSSPAAASATFADLRIHPAVLQATADVGYESPTAIQAATI
                     PALMAGSDVVGLAQTGTGKTAAFAIPILSKIDVTSKATQALVLAPTRELALQVAEAFS
                     RYGARLPQINVLPIYGGSSYSVQLAGLRRGAQVVVGTPGRVIDHLERGTLDLSRVDYL
                     VLDEADEMLTMGFAEDVERILSETPEYKQVALFSATMPPAIRKLTTKYLHDPFEVTAG
                     AKTATAENISQRYIQVAGPRKMDALTRVLEVEPFEAMIVFVRTKQATEEVAEKLRARG
                     FSAAAINGDIVQAQRERTITALRDGGPKGIDILVATDVAARGLDVERISHVVNYDIPH
                     DTESYVHRIGRTGRAGRSGTALLFVSPRERHLLKAIEKATRQTLAEAELPTVEDVNAQ
                     RMAKFADSITDALGGSGIELFRKLVEDYEREHDVPMVDIAAALALQSRDGGAFLMAPD
                     PPPEQRERRERSERPRETRAFTTYRISVGKRHKIGPGAIVGAIANEGGLHRSDFGHIA
                     IGPDFSLVELPATLPKSTLKKLEHTRISGVLIDLRPDRGSTAAFAKAPDKARRTAPSG
                     GGKPRRKRPE"
     gene            3473..4618
                     /locus_tag="VFI55_01780"
     CDS             3473..4618
                     /locus_tag="VFI55_01780"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET7072798.1"
                     /translation="MTLAKERDAKGGLEQIAHVDRVASLTGLRAVAAVLVVATHAAYT
                     TGKYTHGYWGLVGARMEVGVPIFFVLSGFLLFRPWVKSAARGGPPPSLSRYARHRVRR
                     IMPGYVITVLFAYVLYHFREAGPNPGHTWTGLVRNLTLTHIYADGYLGKYLHQGLTQM
                     WSLAVEAAFYVALPLLAYLLLVLLSRRKWRPKLLVGTLTGLMLISPAWLILVHIDRWF
                     PDGARLWLPTYLAWFLAGMLLTVLQEMGVQCYAFVAIPLAVICYFIVSTPIAGAPTTS
                     PTSVGEALVKTVFYAVIAALVVAPLALGAGVEGWYSRFLASRPMVWLGEISYEIFLIH
                     LITMEFAMVYVVHAHVYTGPMLNLFIATLVLTIPLAWLLNRFTRVRD"
     gene            complement(4693..5274)
                     /locus_tag="VFI55_01785"
     CDS             complement(4693..5274)
                     /locus_tag="VFI55_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7072799.1"
                     /translation="MPVDWLAGRRTEVAADRILDAAEQLYTRRDQAAIGMNEIARAAG
                     CSRATLYRYFENREALRTAYVHRETHRLGRAIKEQIDGIDDRRERLVAGVTAALRMVR
                     HSPALASWFAITRPPIGGELAGQSEVITALAAAFVNSLGPEEPAAVERRARWVVRVLT
                     SLLMYPGHDDADERSMIEEFVVPIVLPVPAGPS"
     gene            complement(5274..6515)
                     /locus_tag="VFI55_01790"
     CDS             complement(5274..6515)
                     /locus_tag="VFI55_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7072800.1"
                     /translation="MTAVVSRNPPAMFRLATADTWPNPWPMYRALRDHDPVHHVIPPN
                     RPDHDYYVLSRHADVWSAARDHETYSSAQGLTVSYGDLEMIGLQDNPPFVMQDPPVHT
                     EFRKLVSRGFTPRQVEAVEPKVREFVVERLEKLHADGGGDIVADLFKPLPSMVVAHYL
                     GVPEEDWSQFDGWTQAIVAANTADGGVAGALETVGDAVGSMMAYFTTLIERRRTEPED
                     DTISHLVAAGVGADGDIAGTLSILAFTFTMVTGGNDTVTGMLGGSMPLLDQRPDQREL
                     LVDNPDRIPDAVEELLRLTSPAQGLARTVTRDVTIGDTTVPAGRRVLLLYGSANRDER
                     QYGPDAGELDVSRCPRNILTFSHGAHHCLGAAAARMQSRVALTELLARCPEFEVDESG
                     IVWAGGSYVRRPLSVPIRVRP"
     gene            6656..7126
                     /locus_tag="VFI55_01795"
     CDS             6656..7126
                     /locus_tag="VFI55_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7072801.1"
                     /translation="MSPEEAEFVDRLGLFFELLGATRTMGRLYGWLLICDPPQQSLTQ
                     LANALSVSKASISTVARQLQEGGMVERLPSPNRQHLYRVTSGGFTSVLETQLSLMRLG
                     IEPAEFALSVLREDRVEQRQRVEDFRDFCQFCTQAYRDQLMRMWTEYRETRRQP"
     gene            7123..>7590
                     /locus_tag="VFI55_01800"
     CDS             7123..>7590
                     /locus_tag="VFI55_01800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7072802.1"
                     /translation="MTSTDKVDFSGVGWGSVEWTNLCTLYLRAYESRSPAPILGDKAA
                     AEAVDRIEYDWARMRRAMRPGSNQYMVTMRAKQLDDWSAAFLQSHPNAVVLHLGCGMD
                     TRAFRLDPPDNVLWFDIDQPDVIALRRKLYDDRDGYRMIGSSVTDEAWLDEIPT"
BASE COUNT         1273 a         2484 c         2536 g         1297 t
ORIGIN      
        1 tgcagtttgg cctgcgcggt caatttggcg agggcggcgg acgccaggtc gggcaccggc
       61 ccgttatggt cggcgagctc ggtgatcgtg gtgatcccgg cggcaatgag cttgtcccgt
      121 tgggctaccc ggagaccggc gaccagcagc agatcgtcgg tcgcacgcaa ctgctccgcg
      181 cataacggac aacgaaaaca cgccagaatc gcgtcgtcgt cccagcgcgc cggggagccc
      241 gcggcatagt gccggtcgag cagtcgctgc aacagcgtgc gctgggcgcg gtagaccggg
      301 atcaggtcgt cgatgcggta gcgcacgacg gtgccgtcgc ccagttccaa ctcggcctcg
      361 ggggccacct cgacacccga gctagacagc gcatcggcat atgccgccag ctgcaacaac
      421 gcggtgacct tggccgtgcg tgccagcttg gtgtcggcaa cccgatagcg ctcaccgtcc
      481 agtatcaaaa agtcggcgaa cccgacgaaa cggccgtcga acatcgcggc ctgatacacc
      541 accggtgcgc ggccggcgat ggcgcgttgg gttgcctcgg cggcggccgt cagcccggcg
      601 atggtgtatg ccggacggcc gatgaccgcg accccatccc cgaattcgtc gcgcaattgg
      661 tcgagccggc gtcgttcgtg ctccgtgccc agggtggccg ttcgtgcgag cagctcatct
      721 tcggtgcggt cggtggaaat cgcggggccg cggccgagtt tggcgtcgaa ttcccggagc
      781 agcgcgtact cgcaccgcgc ggcggccgcg agatccgacg cgctgtagac gatgctgccc
      841 cccgtgacga acacagcagc aactgtaggt gagacgtccg acaccgctgg ccaccgaagc
      901 ggccccggac cgctgcggcg ctttcgctct gcggctagtg gccgggggtg ttcccgatga
      961 gctcgacgcc gttgccgttc caatggaacc gcacgtcggt gttcaagccg ttgatgccgc
     1021 cggggtaggt caaggccacc gtgtcaccgg tggtctgcgc ggggtcgatg ccgttgaacc
     1081 cgtaggtgtc gggcaccccc tgggggatga actggccgag atggaacagc accgcccggg
     1141 tggtgggatt gtcggcgttg gtgttggcct tgatgatcac cgccgacagc tgtgcgcact
     1201 cgttgtagtt gcccgccatg ggctcgggac tccagggctg ctgactgcgt ggatcgcgag
     1261 gcagctcgga gaccactttc gcgatggtgg gcgaggcgag attgatcgcg cacgggtctg
     1321 ccggcgcggc gctgctgctg ggtccggctg ccgtcccggg cgccgcgctg ctcggtgcac
     1381 cggcgccagc ggtcgtcgtg gcctgcggcg tcttggcgac cgtggaatct cccgaaccgc
     1441 agccgctcag catcgcggcg gccaagatga cggcggccat gcccgtcagc ggcgcggcgc
     1501 gcttcggtag caacaacaca tggcgcaccg taccgcgatt cgggttcccc ggtgcgacgg
     1561 gcggtgcgcc agacgcggtg acccgccacg ctcagctccg ctgagctcgc gatcaccacg
     1621 ggacttgatg gtggtgaccc gccacgctca gctccgctga gctcgcgatc accacgggac
     1681 ctgatggtgg tgacccgcca cgctcagctc cgctgagctc gcgatcacca ctagactcct
     1741 gggcgatgaa cctctcggac agctcgcccg cggctgcctc tgccacattt gccgacctgc
     1801 ggattcaccc tgcggtcctg caggcgaccg ccgacgtggg ttacgagtcg ccgacagcca
     1861 tccaggcggc gacgattccg gcactgatgg cgggttccga cgtggtggga cttgcgcaga
     1921 ccggcaccgg caagacggcg gctttcgcca tcccgatcct gtccaagatc gacgtgacca
     1981 gcaaagcgac ccaagctctg gtgctggcgc ccacccggga gctggcgctg caggtggccg
     2041 aggcgttcag ccgctacggg gcgcggttgc cccagatcaa cgtgctgccg atttacggcg
     2101 ggtcgtctta cagcgtgcaa ctggccgggc tgcggcgtgg cgcgcaggtc gtggtcggga
     2161 ccccgggccg ggtcatcgac cacctcgaac gcggcacgct ggacctgtcc cgggtggact
     2221 acctggtgct cgacgaggcc gacgagatgc tcaccatggg tttcgccgaa gatgtcgagc
     2281 gcatcctgtc tgaaacgccg gagtacaaac aggtagccct gttctcggcg accatgccgc
     2341 cggcgatccg caagctcacc accaaatatt tgcacgatcc gttcgaagtc accgctggcg
     2401 cgaaaaccgc taccgccgag aacatttcgc agcgctacat tcaagtcgcc ggcccccgca
     2461 agatggatgc gctgaccaga gtcctcgagg tcgagccatt cgaggcaatg atcgtcttcg
     2521 tccgcaccaa acaggccacc gaggaggtcg ccgaaaagct gcgtgcccga gggttttccg
     2581 cggccgccat caacggtgac atcgtgcagg cgcaacgcga gcggaccatc accgcgctgc
     2641 gcgacggggg ccccaaaggc atcgacatcc tggtcgccac cgatgtggcc gcgcggggcc
     2701 tggacgtgga acgaatatcg catgtggtca attacgacat cccgcacgac accgagtcct
     2761 acgtccaccg catcgggcgc accggcaggg cggggcgttc ggggacggcg ctgttgttcg
     2821 tctcgccgcg ggagcgccac ctgctcaagg cgatcgaaaa agcgacgcgg caaacgctcg
     2881 ccgaggcgga attgcccacg gtggaggacg tcaacgccca acgcatggcg aaattcgccg
     2941 attccatcac cgacgcactc ggcggctcgg gcatcgaact gttccgcaag ttggtcgagg
     3001 actacgaacg cgagcacgat gttccgatgg tcgacatcgc cgcggcgttg gcgttgcagt
     3061 cgcgcgacgg cggggcgttc ctgatggcgc ccgatccgcc gccagaacag cgcgaacgtc
     3121 gggaacgtag cgaaaggcca agggaaacca gagcattcac cacctaccgg atctcggtcg
     3181 gcaaacgtca caagatcggt cccggtgcga tcgtcggcgc catcgccaac gagggcggtc
     3241 tgcatcgcag cgatttcggc cacatcgcca tcggaccgga cttctcgctg gtggaattgc
     3301 cggccacgct ccccaagtcg acgctgaaaa agctcgagca cacgcggatc tcgggcgtac
     3361 tgatcgacct gcggccagat cgcggctcta cggcagcctt cgcgaaagcc cctgacaaag
     3421 cgcgtcgcac tgccccatcg gggggcggca aaccgcgcag aaaacgcccc gaatgaccct
     3481 cgccaaggaa cgggacgcca agggcgggct cgagcaaatc gcccacgtgg accgggtcgc
     3541 gtcgctcacc ggcctccgcg cggtcgccgc cgtgctcgtc gtcgccaccc acgcggcgta
     3601 caccaccggc aagtacaccc acggctactg gggtctggtc ggcgcccgga tggaggtcgg
     3661 cgttccgatc ttcttcgtgc tgtctggttt cctgctgttc cgcccgtggg tgaaatcagc
     3721 cgccaggggc ggtccgccgc cttcgctgag tcgctatgcg cggcaccggg ttcggcgcat
     3781 catgcccggc tacgtcatca ctgtgctgtt cgcctacgtt ctgtatcact tccgggaagc
     3841 gggaccgaac cctgggcaca cctggacggg actggtgcgc aacctcacgt tgacgcacat
     3901 ctacgccgac ggctacctgg gcaagtacct gcatcagggc ctgactcaga tgtggagcct
     3961 ggcggtagag gcggcctttt acgtcgcact accgctgctt gcctacctgc tgctggtgct
     4021 gttgagtcgg cggaaatggc ggccgaagct gctggtcggc acgctgacgg gactgatgct
     4081 gatcagcccg gcgtggctga tcctggtgca catcgaccgc tggtttcccg atggcgcccg
     4141 gctgtggcta ccgacctacc tcgcttggtt cctcgcgggc atgttgttga cggtgctgca
     4201 ggaaatgggt gtgcagtgct acgcgttcgt cgccataccg ttggcggtca tctgctattt
     4261 catcgtctcc acacccatcg cgggtgcgcc caccacctcg ccgacatcgg tgggggaggc
     4321 gctggtcaag acggttttct acgccgtgat cgccgcgctg gtggtggcac cgctggccct
     4381 gggtgccggt gtcgaaggat ggtattcccg gtttctagct tcccggccca tggtgtggct
     4441 gggtgagatc tcctacgaga tcttcctgat ccatctcata acgatggaat tcgccatggt
     4501 ctacgtggtt cacgcgcatg tgtacaccgg cccgatgctg aatctgttca tcgcgacgct
     4561 ggtgttgacg attccgctgg cctggctgtt gaaccgtttc acgcgcgtcc gggattgagg
     4621 gttgcggcca cgccggccag gccgcgatcg tcacgtccgc ccctcggcag tggtgccgcc
     4681 aagccgcacg aatcaagacg gaccggcggg aaccggcagc acgatcggca cgacaaactc
     4741 ctcgatcatg gaccgctcgt cggcgtcgtc gtggccgggg tacatcaaca gcgaggtgag
     4801 cacgcgcacc acccaacgcg cccggcgctc gacggcggcg ggttcttcgg gaccaagcga
     4861 gttgacgaag gcggcggcca aggcggtaat cacctcggac tgcccggcca gctcgccgcc
     4921 gatcggggga cgggtgatgg caaaccacga tgccaacgcg ggactgtgcc gcaccattcg
     4981 caacgcggcg gtgacgccgg cgacgagccg ctcgcgccgg tcgtcgatac cgtcgatctg
     5041 ctccttgatc gcgcgaccga gccggtgcgt ctcgcggtgc acgtaagcgg ttcgcagcgc
     5101 ctcgcggttc tcgaagtagc ggtacagcgt cgcgcgggaa cagcctgcgg ccctggcgat
     5161 ttcgttcatg ccgatggcgg cctggtcgcg ccgagtgtaa agctgctcgg ccgcgtcgag
     5221 tatccggtcc gcggctactt ccgtgcggcg cccggccagc cagtcgaccg gcatcagggt
     5281 ctcactcgaa tcggcaccga tagcggacga cggacatagc tgccgccggc ccacacgatc
     5341 ccggattcgt cgacctcgaa ctccgggcat cgcgccagca gttcggtcag cgcaacccgc
     5401 gattgcatcc gggccgcagc ggctcccagg cagtggtggg caccgtggct gaaggtcagg
     5461 atgttgcgcg gacaccggga cacgtcgagt tcacctgcgt ccggtccgta ttggcgttcg
     5521 tcccggttgg ccgacccata gagcagcaac acccttcgtc cagccgggac ggtggtgtcg
     5581 ccgatggtga cgtcgcgggt gaccgtgcgc gccagtcctt gcgccggcga ggtcagccgt
     5641 agcagttcct cgacggcgtc ggggattcgg tccgggttgt ctaccaacaa ctctcgctgg
     5701 tccggccgtt gatcaagcag cggcatcgag ccgcccagca tgccggtgac cgtgtcgttg
     5761 ccgccggtga ccatggtgaa cgtgaacgcc agaatggaca gcgtgccggc gatgtcgccg
     5821 tccgcgccga ccccggcggc gaccaggtgc gagatggtgt cgtcttcggg ttcggtccgg
     5881 cgccgctcga tcagggtggt gaagtaggcc atcatcgacc cgacggcatc gccaaccgtt
     5941 tccagtgcac ccgcgacgcc gccgtcggcg gtgttggccg cgacgatcgc ctgggtccag
     6001 ccgtcgaact gcgaccagtc ctcctcgggc acaccgaggt aatgcgcgac caccatcgac
     6061 ggcagcggtt tgaacaggtc cgcgacgatg tctccaccgc catcggcgtg cagcttctcg
     6121 aggcgttcga cgacgaattc acgcaccttc ggctccaccg cctcgacctg ccggggggtg
     6181 aacccgcggg aaaccagttt gcgaaactcg gtgtgcaccg gtggatcctg catgacaaac
     6241 ggtggattgt cctgcagacc aatcatttcc aagtcgccgt agctgacagt cagcccctgc
     6301 gcggaggagt aggtctcgtg gtcgcgggcc gctgaccaca cgtcggcgtg ccgcgacagc
     6361 acgtaatagt cgtgatcggg gcggttcggg gggatgacgt ggtgcaccgg gtcgtggtcg
     6421 cgcagcgcgc ggtacatcgg ccacggattc ggccaggtgt cggcggtggc gagccggaac
     6481 atcgcgggcg ggttccgaga cacaacggca gtcatgtctc atgcgtacga catagcgagc
     6541 aagacgtcaa ccatcgataa ggggccggcc ccggtagggc cggcgtcact tcctcaagcg
     6601 cgagcatccg cgtaaactat gtttagttcg tactgaacaa acgtgcggga ggcttatgtc
     6661 accggaagag gccgagttcg tcgaccgcct cgggctgttc ttcgagctcc tcggtgccac
     6721 ccgcaccatg ggtcggctat atggatggct cctgatctgt gatccgccgc agcagtcttt
     6781 gacccagctg gccaatgcgt tatcggtgag taaggcatcg atcagtaccg tcgcgcggca
     6841 gctgcaggaa ggcgggatgg tggagcggct gcccagcccc aaccgtcaac acctctaccg
     6901 ggtcacgtcg ggcgggttca ccagcgttct ggaaacgcag ctgtcgctga tgcgtctggg
     6961 catcgagccc gccgagttcg ctctgtctgt gctgagggag gaccgcgtcg aacagcggca
     7021 gcgggtcgag gacttccgcg atttctgcca gttctgcacc caggcctacc gcgaccagct
     7081 catgcggatg tggaccgaat atcgggagac aaggagacag ccatgacttc gacggacaag
     7141 gtcgacttca gtggcgtggg gtggggctcg gtggaatgga cgaatctgtg cacactgtac
     7201 ctgcgggcat atgaaagccg ttcgccggca ccgattctcg gagacaaggc ggcagccgag
     7261 gcggtggacc gcatcgaata cgactgggcc cgcatgcgcc gcgccatgcg gcccggatcc
     7321 aatcaatata tggtcaccat gcgcgccaaa cagcttgacg actggagcgc cgccttcctc
     7381 caaagccatc cgaacgccgt cgtgctgcac ctcgggtgcg gcatggacac ccgggcgttc
     7441 cggctggatc cacccgacaa cgtgctgtgg ttcgacatcg atcaaccgga tgtaatcgcg
     7501 ttgcggcgca agctgtatga cgaccgcgac ggctaccgga tgatcggctc gtcggtgacc
     7561 gacgaggcgt ggctggacga aattcccacc
//
LOCUS       DASSGQ010000039         4593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_883965,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000039 DASSGQ010000000
VERSION     DASSGQ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883965"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2637)
                     /gene="glgP"
                     /locus_tag="VFI55_01805"
     CDS             complement(<1..2637)
                     /gene="glgP"
                     /locus_tag="VFI55_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HET7072803.1"
                     /translation="MKALRRFTVRAHLPERLAALDQLSTNLRWSWDKPTQDLFATIDP
                     KLWARCAADPVALLGAVNPARLDELALDEGFLGRLDELAADLNDYLSRPLWYQQQQHE
                     GVAMPAAVAYFSMEFGVAEVLPNYSGGLGILAGDHLKSASDLGVPLIAVGLYYRSGYF
                     RQSLTADGWQHETYPSLDPQGLPLRLLTDSTGDPALVELTLPDSAQLRARIWVAQVGR
                     VPLLLLDSDVPENEHELRGVTDRLYGGDQQHRMVQEILAGIGGVRAIRAFTAIEELPA
                     PEVFHMNEGHAGFLGVERIRELMTDSGLDFDTALTVVRSSTVFTTHTPVPAGIDRFPV
                     DMVRLYFDDHHDGNRKKNRDGEAALALLPGVPTSRIIALGAEDDPTKFNMAHMGLRLA
                     QRANGVSLLHGQVSRVMFNELWPGFDPDEVPIGSITNGVHARTWAAPEWLQLGRELAG
                     SDSFGDPGVWLRLQQVDPGHLWWIRSQLRSLLVEDVRVRLRRSWLERGASDAELGWIA
                     TAFDPSVLTIGFARRVPTYKRLTLMLRDPERLERLLLNRERPIQLIVAGKSHPADDGG
                     KALIQQVVRFADRPQVRHRIAFLPDYDMSMARLLYWGCDVWLNNPLRPLEACGTSGMK
                     SALNGGLNLSIRDGWWDEWYDGENGWEIPSADGVTDESRRDDLESSALYNLLEQAVAP
                     KFYERDEHGLPPRWIEMVRHTLQTLGPKVLASRMVRDYVEQYYLPAAESVRRTVGAPE
                     GAEREGDREFGAARELAAYRRRVEEAWPKIEITDVDSTGLPDTPVLGSKLTLTATVQL
                     AGLRPDEVTVQAVIGRVDGTDVLFDPVTVDMSYTGTAEGGNQVFSTTTPLPLAGALGY
                     TVRVLPHHPMLAASNELGLVTLA"
     gene            2979..>4593
                     /locus_tag="VFI55_01810"
     CDS             2979..>4593
                     /locus_tag="VFI55_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003915171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HET7072804.1"
                     /translation="MPGRVEIDNVQPVVSCGAYPAKAVVGETIPVSAAVWREGHEAVA
                     ATLVVRYLGPRYPQVTEVRQVRAVQAPELPAAAFDPKAAEQRRVKPLLLPMTEGQEPY
                     VFHGQFTPDRVGLWTFRVDGWGDPIHTWRHGLIAKLDAGQGETELSNDLLVGAALFER
                     AATGVPRAQRDPLLTAAAALRTPGDPVTRTALALAPEIEELIATYPLRDIVTRGEQFG
                     VWVDRPLGRFGSWYEMFPRSTGGWDADGKPVHGTFATAAAQLPRIADMGFDVVYLPPV
                     HPIGKIHRKGRNNSPTAAAGDVGSPWAIGSDEGGHDAIHPKLGTIDDFDDFVSAARDL
                     GMEVALDLALQCAPDHPWAREHRQWFTELPDGTIAYAENPPKKYQDIYPLNFDNDPAG
                     LYDEVLRVVRHWVDHGIKFFRVDNPHTKPPNFWAWLIGQVKDTDPDVLFLSEAFTPPA
                     RQYGLAKLGFTQSYSYFTWRTAKWDLTEFGNDIAALADFRRPNLFVNTPDILHAVLQH
                     DGPGMFAIRAVLAATMSPAWGVYSGYELFE"
BASE COUNT          816 a         1566 c         1473 g          738 t
ORIGIN      
        1 agccagggtg acgagaccga gctcgttgct ggcagcgagc atcggatggt ggggcagcac
       61 gcgcaccgtg tatcccaacg ctcccgccag cggcagcggc gtcgtcgtcg agaagacttg
      121 gttgcctcct tcggcggtgc cggtgtagga catgtcaacc gtgaccggat cgaacagcac
      181 gtcggtgccg tcgactcggc ctatcaccgc ctgcaccgtg acttcgtcgg gccgcagccc
      241 cgccagctgc acggtcgccg tcagggtgag cttcgagccg agcaccggag tgtcgggcag
      301 gccggtgctg tcgacatcgg tgatctcgat cttcggccat gcctcctcga cgcgccggcg
      361 gtaggcggcc agctcgcggg cggcgccgaa ctcgcgatcg ccctcacgtt cggcgccttc
      421 gggcgcaccg acggtcctgc gcaccgactc cgccgccggc aggtagtact gctcgacata
      481 gtcgcgcacc atccgtgacg ccagcacctt cggtcccagg gtctgcaagg tgtggcggac
      541 catttcgatc caccggggcg gcagcccgtg ttcgtcacgc tcgtagaact tcggtgccac
      601 cgcttgttcc agcaagttgt agagcgcgct cgactccagg tcgtcgcgcc ttgactcgtc
      661 ggtcacaccg tcggcagacg gtatctccca accgttttcg ccgtcgtacc attcgtccca
      721 ccagccgtcg cggatggaca ggttcagccc gccgttcagg gcgctcttca ttcccgaggt
      781 gccgcaggcc tccagtggcc gcagcgggtt gttgagccag acgtcgcagc cccaatagag
      841 cagccgggcc atcgacatgt cgtagtcggg gaggaaggcg atgcggtggc gcacctgcgg
      901 gcggtcggcg aaacggacga cctgctggat cagcgccttg ccgccgtcat cggctgggtg
      961 cgatttaccg gcgacgatca gctggatcgg cctttcacgg ttgagcagca gccgttccag
     1021 ccgttccggg tcgcgcagca tcagtgtcag gcgcttgtac gtcggcaccc gccgcgcgaa
     1081 cccgatggta agcacgctcg gatcgaacgc cgttgctatc caaccaagtt cggcatccga
     1141 ggcgccgcgc tccagccacg aacgccgcag tcgcacgcgg acatcctcga ccagcagtga
     1201 ccgcaattgc gagcggatcc accacagatg gccagggtcg acctgctgca gtcgcaacca
     1261 gacgccgggg tcaccgaacg agtcggaccc ggccagctcg cgccccagct gcaaccattc
     1321 cggcgcggcc caggtccgcg cgtgaacccc gttggtgatc gacccgatcg gcacctcgtc
     1381 cgggtcgaat ccgggccaca gctcgttgaa catgacccgg ctcacctggc cgtgcagcaa
     1441 cgaaaccccg ttggcgcgct gcgccagccg cagacccata tgagccatgt tgaatttggt
     1501 cggatcgtcc tcggcgccga gcgcgataat ccgggaggtc ggcacaccgg gcagcaacgc
     1561 gagcgcggcc tcgccatccc gattcttttt gcgattgcca tcgtgatggt cgtcgaaata
     1621 gagccgcacc atgtcgaccg ggaatcggtc gatacccgcg ggcaccgggg tgtgggtagt
     1681 gaacacggtg ctggaccgca ccaccgtcaa cgccgtgtcg aagtccagtc ccgaatcggt
     1741 catcagttca cgaatgcgtt ccactccaag gaacccggcg tggccctcgt tcatgtggaa
     1801 tacctcaggt gccggcaact cttcgatcgc ggtgaacgcg cggatcgccc gcaccccgcc
     1861 gatgccggcc agaatctcct gcaccatccg atgttgctga tcgccgccgt acaggcggtc
     1921 ggtgacgcca cgcagctcgt gctcgttttc ggggacgtcg gaatcgagca acagcagcgg
     1981 aacacgaccc acctgcgcga cccagatccg tgcccgcagc tgcgccgaat cgggaagcgt
     2041 cagctcgacc agcgcgggat cgccggtcga gtcggtgagc agccgcaacg gtagcccctg
     2101 ggggtccagc gacggatagg tttcgtgctg ccagccgtcc gcggtcagcg actgccggaa
     2161 gtaacccgag cggtagtaga gacccaccgc gatcagcgga acccccagat cggacgcgga
     2221 cttcaggtgg tcgcccgcca gaatccccaa gccgcccgag taattgggca gcacctcggc
     2281 gacgccgaac tccatcgaga agtacgcgac ggcggccggc atcgcgacac cttcgtgctg
     2341 ctgctgctgg taccacagcg gacggctcag gtaatcgttg aggtcggccg ccagctcatc
     2401 gagccggccc aggaatcctt cgtcgagcgc caactcgtcg agccgcgccg ggttcaccgc
     2461 accaagcagg gccaccggat cggcggcgca ccgcgcccac agcttcgggt cgatggtcgc
     2521 gaacaggtcc tgagttggct tgtcccacga ccagcgcagg ttggtcgaca actggtcgag
     2581 cgcggccaga cgctcgggca gatgagcgcg gacggtaaag cggcggaggg ctttcacgcg
     2641 tcattagctt actgaggatt ccacagctcg cagtgctagt gacccaccgt ttacgttcag
     2701 cgtgtgtgac cgaacactag ggtgggtacc gggtagttat tggggcggca acgaagctcc
     2761 ccatcacgaa gccccccgaa cgacaggaag tatcgcaccg agagggcatt ccgcgacgag
     2821 cacaccgcgg tttggccgca cgcaatcgga acaccaccgg cgacgatgcg gcccggacag
     2881 cgggccgagg aggagccggg gaaatcgaaa caccaccggc gacgatgcgg cccggacagc
     2941 gggccgagga ggagccggga aatcgaaacg gagtggttgt gcccggtcgt gtcgagatcg
     3001 ataacgtcca gcccgtcgtt tcctgcggcg cctaccccgc caaggccgtg gtcggtgaga
     3061 ccatcccggt cagcgccgcg gtatggcgcg agggccacga ggccgtcgcc gccaccttgg
     3121 tcgtgcgcta cctgggcccg cgctatccgc aggttacgga agtcagacag gtcagggcgg
     3181 ttcaggctcc cgaactgccc gccgcggcat tcgatcccaa ggccgccgag caacgacggg
     3241 tcaagccgtt gctactcccg atgaccgagg gtcaagagcc ttacgttttc cacggccagt
     3301 tcaccccgga ccgggtcggg ctgtggacgt tccgggtcga tggctggggt gaccccatcc
     3361 acacctggcg gcacgggctg atcgcaaagc tggacgccgg gcagggcgag accgaactgt
     3421 ccaacgatct actggtcgga gcggcactgt ttgagcgtgc cgcgaccggt gtgccgcgcg
     3481 cgcaacgcga tcccctgctg accgcggccg cggcgctgcg gacccccgga gatccagtga
     3541 cacgcaccgc gctggccctg gctcccgaga tcgaagagct gatagcgacc tacccgttac
     3601 gcgacatcgt cacgcggggc gaacagttcg gggtgtgggt ggatcggccg ctgggccgct
     3661 tcggctcgtg gtacgaaatg tttccgcgct cgaccggcgg gtgggacgcc gacggcaagc
     3721 ccgtgcacgg caccttcgcc accgcggccg cgcaacttcc gcgcatcgcg gacatggggt
     3781 tcgacgtcgt ctacctgccg cccgtgcatc cgatcggcaa gatacaccgc aagggccgca
     3841 acaactcccc caccgccgcc gcaggagatg tgggttctcc gtgggcaatt ggcagcgacg
     3901 agggtggcca cgatgcaatt cacccgaagt tgggcacgat cgacgacttt gacgacttcg
     3961 tatctgcggc acgcgatctg ggcatggagg tggcgctcga tctggcgctg caatgcgcac
     4021 cggaccatcc gtgggcacgc gaacatcggc agtggtttac cgaattgccg gatgggacca
     4081 ttgcctacgc ggagaacccg ccgaagaaat atcaggacat ctatccactc aacttcgaca
     4141 acgatcccgc gggtctctac gacgaagtgc tgcgcgtggt ccggcactgg gtcgaccacg
     4201 gcatcaagtt ctttcgggtc gacaatccgc acaccaagcc ccccaacttc tgggcctggc
     4261 tgatcggtca ggtcaaagac accgaccccg acgtgctctt cctgtccgag gccttcaccc
     4321 cgcccgcccg ccagtacggc ctggccaaac ttggcttcac ccagtcctac agctacttca
     4381 cctggcggac ggccaaatgg gacctcaccg aattcggcaa tgacatcgcc gccctcgccg
     4441 atttccgccg gcccaacctg ttcgtcaaca cccccgacat cctgcacgcg gtactgcaac
     4501 acgacggccc gggaatgttc gccatccgcg cggtgctggc ggcgacgatg agcccggcgt
     4561 ggggagtcta ttccggctac gagctgttcg aac
//
LOCUS       DASSGQ010000040        20720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_489055,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000040 DASSGQ010000000
VERSION     DASSGQ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 20720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20720
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489055"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1251)
                     /locus_tag="VFI55_01815"
     CDS             complement(16..1251)
                     /locus_tag="VFI55_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013830050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HET7072805.1"
                     /translation="MGLTLAERRAVTEMTAIRYVVADRRVKSRILDELCANTGWHRNH
                     ARKALKAALQPKVVAPRRSPRPPVYGPDVIAALTACWLVLGMPAGKRLAPMLNELVAV
                     LRHFGELVIDDDTAELLVSMSAATIDRRLAGERAKRQLKGHRATKPGSLLRSQIPIHT
                     WADWDDAKPGFVEIDLVSHDGGNTIGPFAFTLTVTDVATGWTENRSVPSKEAKCVLSA
                     LESIANKMPFPILGVDSDNGAEFINVYLFVWCRNRKITFTRARPTNKNDGCHVEQKNW
                     AVVRTVVGYHRYDTAPELLLLNEIWQLQSQLTNYFYPQQKLISKVRDGAKVTKKHDTA
                     TTPFHRAINHPDTTVDHIAALARTHALINPAATQRQIQALTTQLHKMTTSKFQPATRA
                     RVPKRAQSHEATEPPSRAS"
     gene            1389..2742
                     /locus_tag="VFI55_01820"
                     /pseudo
     CDS             1389..2742
                     /locus_tag="VFI55_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384814.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
     gene            complement(2772..3866)
                     /locus_tag="VFI55_01825"
     CDS             complement(2772..3866)
                     /locus_tag="VFI55_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HET7072806.1"
                     /translation="MTDDATKRGDQAIVVRDAYKHYGDFVALDHVDFVVPTGSLTALL
                     GPSGSGKSTLLRTIAGLDQPDTGTVTINGRDVTRVPPQRRGIGFVFQHYAAFKHMTVR
                     DNVAYGLTIRKRPKAEIKQKVDNLLEVVGLSGFQTRYPNQLSGGQRQRMALARALAVD
                     PQVLLLDEPFGALDAKVREDLRAWLRRLHDEVHVTTVLVTHDQSEALDVADRIAVLNK
                     GRIEQVGSPTEVYDSPANAFVMSFLGAVSTLNGALVRPHDIRVGRNPDMAVATADGTA
                     ESTGVLRATVDRVVVLGFEVRVELTNAATGGAFTAQITRGDAEALALRDGDTVYVRAT
                     RVPPVLGGLAEPAGDGAVGAGEDQNALTSA"
     gene            complement(3888..4700)
                     /gene="cysW"
                     /locus_tag="VFI55_01830"
     CDS             complement(3888..4700)
                     /gene="cysW"
                     /locus_tag="VFI55_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HET7072807.1"
                     /translation="MTSSPGVRYLIRFVALAYIFVLLVVPVTLILWRTFQPGLGQFFD
                     WVSTPAAISALNLTLLVVAIVVPLNVIFGVPTALALARNRFRGKGVLQAVIDLPFAVS
                     PVIVGVALIVLWGSGGVLGFVEKDLGFKIIFGLPGIVLASIFVTLPFVVREVEPVLHE
                     LGTDQEQAAATLGSGSWQTFWRITLPSIRWGLTYGVVLTVARTLGEYGAVIMVSSNLP
                     GVSQTLTLLVSDRYNRGAEYGAYALSTLLMCVAVVVLIFQVVLDARRARATK"
     gene            complement(4697..5584)
                     /gene="cysT"
                     /locus_tag="VFI55_01835"
     CDS             complement(4697..5584)
                     /gene="cysT"
                     /locus_tag="VFI55_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HET7072808.1"
                     /translation="MTGSFLSSSANPEAIRPELEAAPAPPAARRVRGLDATSLRLGVA
                     MLWLSVIVLLPLAAIVWQAAGGGWEDFWLAVTSHAAVESFRVTLVISSAVTVLNLVFG
                     LVIAWVLVRDDFVGKRLVDAIIDLPFALPTIVASLVMLALYGHNSPVGIHLQHTPWGV
                     GVALAFVTLPFVVRAVQPVLLEIDRETEEAAASLGATGPKIFTSVVLPSLLPALLSGA
                     GLAFSRAIGEFGSVVLIGGAVPGKTEVSSQWIRTLIENDDRTGAAAISIVLLTISFVV
                     LLLLRVVGARAAKREESAA"
     gene            complement(5581..6627)
                     /locus_tag="VFI55_01840"
     CDS             complement(5581..6627)
                     /locus_tag="VFI55_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7072809.1"
                     /translation="MLNDIRGGPRWRPVRFVGVLTLIVGVVAACQGGASDVVGGGGLT
                     AAHTSLTLVAYSAPEPGWSKVIPAFNASDEGKSVQVITSYGASGDQSRGVVDGKPADL
                     VNFSVEPDITRLVKAGKVSKDWNTDATKGIPFGSVVALVVRAGNPKNIKDWDDLLRPG
                     IQVITPSPLSSGSAKWNLLAPYAVKSQGGNNGQAGVDFIKKLVGDHVKLRPGSGREAT
                     DVFVQGSGDVLISYENEAIATERAGKPVQHVIPQQTFKIENPLAVVNTSPHLQAATAF
                     KNFQYTAAAQKLWAQAGFRPVDPSVSADFRDQFPVPEKLWTIADLGGWTTVDPQLFDK
                     NTGSITKIYTQATG"
     gene            complement(6964..7158)
                     /locus_tag="VFI55_01845"
     CDS             complement(6964..7158)
                     /locus_tag="VFI55_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072810.1"
                     /translation="MEAFLNWWDGNELWLSGLPFVLQATVVMPVVLAVAYITAELLDR
                     LLGKGIQLMRRVRHADGAPG"
     gene            7212..8447
                     /locus_tag="VFI55_01850"
     CDS             7212..8447
                     /locus_tag="VFI55_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7072811.1"
                     /translation="MPTASFDSAATTCLIAGGGPAGMMLGLLLARAGVQVTVMEKHAD
                     FLRDFRGDTVHASTLGLLDELGLGPRFAQLPHRNIDTVRMEIQGDPVNLDLRRLPGAH
                     QYIALVPQWDFLEMIAGAAEAEPTFRLLRSTEVTGVIRDGRRVLGVTYRDQTGEAKEM
                     RATLTVACDGRTSTVRSAVGLVPRSFGAPMDVWWFRLPRRPDDPSGLAGVLRSGHASI
                     MIDRGDYYQIAYIIPKGADAQMRAQGVEALKSALVGMVPWIADRVDTLTSFDDVKLLD
                     VELNRLRRWYSDGVLCIGDAAHAMSPVGGIGINLAVADAVAAARILAGPLRAGRVSTG
                     RLARVQARRWLPAALLQAAQRSIHANVIAAAVAGGDRPPPRAVRAVSRSPALRRFAGY
                     LVAIGPLPEHAPRYARRAG"
     gene            8635..10599
                     /locus_tag="VFI55_01855"
     CDS             8635..10599
                     /locus_tag="VFI55_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET7072812.1"
                     /translation="MTSAPPLEFSSAGPLATSASLRNPFPPIADYAFLSDWETTCLIS
                     PAGSVEWLCLPRPDSPSVFGAILDRSAGHFRLGPYGVSVPSARRYLPGSLIMETTWQT
                     PTGWLIVRDALVMGPWHDIERRSRTHRRTPMDWDAEHILLRTVRCVSGTVEVMMSCEP
                     AFDYHRVGAAWEYSANAYGEAIARASRQPDAHPTLRLTTNLRIGLEGREARARTRMRG
                     GDDVFVALSWTKHPAPQTYEEAADKMWQTTECWRQWINIGNFPDHPWRAYLQRSALTL
                     KGLTYSPTGALLAASTTSLPETPQGERNWDYRYAWVRDSTFALWGLYTLGLDREADDF
                     FAFIADVSGANSNERHPLQVMYGVGGERSLVEDELNHLSGYDHARPVRIGNGAYDQVQ
                     HDIWGSILDSFYLHAKSREQIPETLWPVLKKQVEEAIKHWREPDRGIWEVRGEPQHFT
                     SSKVMCWVALDRGSKLAERQGEKSYAQQWRAIAEDIKADILEHGVDSRGVFTQRYGSD
                     ALDASLLLVVLTRFLPPDDPRVRNTVLAIANELTEEGLVLRYRVEETDDGLSGEEGTF
                     TICSFWLVSALVEIGEVTRAKRLCERLLSYASPLHLYAEEIEPRTGRHLGNFPQAFTH
                     LALINAVVHVIRAEEEADGSGTFQPANAPV"
     gene            complement(10634..11392)
                     /locus_tag="VFI55_01860"
     CDS             complement(10634..11392)
                     /locus_tag="VFI55_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HET7072813.1"
                     /translation="MRILGPAAALTVMAALVAGCGSVVDGTAKPAPNLKPRPLSGATV
                     QQVLLDGTTLSQMLSQPFVSRRPAEFGGPDKLTHRDGPVASPGCLGVTTMLQQSVYQS
                     ADVKDVASESWWNNGEPAQVIGVQEGVVSLPSAEQAQVLFATFSAQWQQCKGTTMTEQ
                     SGPISTTTDINDVRVAGSVLAATNTATAVLPNMPALRPTPQARAIGVQLNCLVEVEVV
                     FFGERRSSDPGSANVDTSATDIAKAMMDKVNALS"
     gene            complement(11395..13299)
                     /gene="lepA"
                     /locus_tag="VFI55_01865"
     CDS             complement(11395..13299)
                     /gene="lepA"
                     /locus_tag="VFI55_01865"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET7072814.1"
                     /translation="MPSTTGAQEIPISSFADKTFTAPAQIRNFCIIAHIDHGKSTLAD
                     RMLQFTGVVDERSMRAQYLDRMDIERERGITIKAQNVRLPWRVSAGEDAGKDYVLHLI
                     DTPGHVDFTYEVSRALEACEGAVLLVDAAQGIEAQTLANLYLALDRDLHIIPVLNKID
                     LPAADPDRYAGEIAHIIGCEPSDVLRVSGKTGEGVADLLDHVVREVPPPQGDADAPTR
                     AMIFDSVYDIYRGVVTYVRVVDGKITPRERILMMSTGATHELLEVGIVSPDPKPSEGL
                     GVGEVGYLITGVKDVRQSKVGDTVTTARHGAKEALTGYREPKPMVYSGLYPVDGSDYP
                     DLRDALDKLQLNDAALTYEPETSVALGFGFRCGFLGLLHMEITRERLEREFDLDLIST
                     SPNVVYRVILEGRAGGADDSIVVTNPSDWPEGKIRTVYEPVVKTTIIAPSEFIGTIME
                     LCQSRRGELGGMDYLSPERVELRYTMPLGEIIFDFFDSLKSRTRGYASLDYEEAGEQE
                     AQLVKVDILLQGEAVDAFSAIVHKDGASAYGNKMTTKLKELIPRQQFEVPVQAAIGSK
                     IIARENIRAIRKDVLSKCYGGDITRKRKLLEKQKEGKKRMKTIGRVEVPQEAFVAALS
                     SDAAGDKGKK"
     gene            13408..14010
                     /locus_tag="VFI55_01870"
     CDS             13408..14010
                     /locus_tag="VFI55_01870"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HET7072815.1"
                     /translation="MASARKSQWRTFQRFAENLVFNGAPRLVRHVQTSQIVLRELQQA
                     VKITANVIAAVAPPPPASITTGRPVTSTSFPTAQRARRLVYAPDLDGRADPGEVVWTW
                     VVYEDDPTQGKDRPVLVVGRDRAVLLGLMMSSQERHAADPDWVRIGSGAWDYEGRESW
                     VRLDRVLDVPEEGIRREGAILDREIFDVVAARLRANYSWS"
     gene            complement(14100..14528)
                     /locus_tag="VFI55_01875"
     CDS             complement(14100..14528)
                     /locus_tag="VFI55_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7072816.1"
                     /translation="MRIADVLRNKGAAVVTINPDATVQELLAGLAEQNIGAMVVVGAE
                     GVVGIVSERDVVRQLHKHGPSVLSRPISKIMTSAVATCTKSDTVDAISVLMTKNRVRH
                     VPVLDGKKLIGIVSIGDVVKTRMEELEAQQQQLHSYITQG"
     gene            14740..15582
                     /locus_tag="VFI55_01880"
     CDS             14740..15582
                     /locus_tag="VFI55_01880"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HET7072817.1"
                     /translation="MIEITLLGTGSPIPDPNRAGPSTLVRVSGQVFLVDCGRGVLQRA
                     AAVGVGAAGLSALLLTHLHSDHIGDLGDLLITRWISTFAPDPTPLPIIGPPGTAETVE
                     ATLKAFGHDIGYRMAHHADLNAPPAVEVFECTEGPAWDLDGVSIRVAPTDHRPVAPTI
                     GFRIESGTTAVVLAGDTVPCAGLDDLATGADALVHTVIRKDIVANIPQQRLRDICDYH
                     SSVEQAAATAARAGVGTLVMTHYVPALVPGQEEQWRALAASEFGGRVELGDDLHRVEV
                     NARA"
     gene            complement(15586..16323)
                     /locus_tag="VFI55_01885"
     CDS             complement(15586..16323)
                     /locus_tag="VFI55_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072818.1"
                     /translation="MNARTDGTGPGRGRARQGGPPLVLLSAVCLVLLFGGLAIGVALG
                     GVMPLPYGPAAAVQAYVRAQPVAVQVIATAAFASSVPLALYAATASARLRQLGVTAPG
                     GTIALTGGTLAAGALGLTGLLAWTLSRPEVSADAALVRALYFLVFLVGGPGHIVALGL
                     LVAGMAVPSLVLGLTPRPVAWMGVAIAALAELTTLVLIWPPLAVILPIARVSALVWLV
                     AVGALLPLRRSDVRSREAPSVRAAVRE"
     gene            complement(16359..17201)
                     /locus_tag="VFI55_01890"
     CDS             complement(16359..17201)
                     /locus_tag="VFI55_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HET7072819.1"
                     /translation="MWRLRVVHTTGYAYQSPVTASYNEVRLTPRSDTRQNVVLNRIET
                     IPATRSYRYIDYWGTAVTAFDLHAPHTDLTVTSSSVVETERPEPPAAKVSWDDLESEA
                     VIDRYDELLRPTAHTPNSNKRVASVAKRIAKAYGPRDAVVAVGDWVRRELDYLPGTTG
                     VHSSGLDALSEGKGVCQDFVHLSLIVLRSMGIPGRYVSGYLHPKPNAVVGDTVEGRSH
                     AWIQAWTGDWWNYDPTNDAEITEQYVSVGAGRDYTDVSPLKGIFSGQGATDLDVIVEI
                     TRLA"
     gene            complement(17201..18178)
                     /locus_tag="VFI55_01895"
     CDS             complement(17201..18178)
                     /locus_tag="VFI55_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HET7072820.1"
                     /translation="MLARNAEALYWIGRYVERADDTARILDVALHQLLEDSSVDPDQA
                     SRLLLRVLGIDPPDHDLDVWSLTDLVAFSTNVSGGCSIVDAITAARENAKSAREVTSS
                     EIWECLNTTYHALAERERAAKRLGPHEFLSYVEGRAAMFAGLADSTLSRDDGYRFMSL
                     GRAIERVDMTVRLLLSRFGDSASSPAWVTLLRSAGAHDTYLRTYRGVLDANRVVEFML
                     LDRLFPRSVFYSLRMAEHNLHELAHDPQSRIGATAEAQRLLGRARSELEFVEPGVLLE
                     SLESRLADLQRTCYDVGEALSLQYFHVTPWVAWSDAGQRGRLVARQGEA"
     gene            complement(18178..19872)
                     /locus_tag="VFI55_01900"
     CDS             complement(18178..19872)
                     /locus_tag="VFI55_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HET7072821.1"
                     /translation="MSKVSLPGQLEENRRGSRGGAGRPNARSERIFGGYNTSDSYSAA
                     FDEMFDAQGNVRGPYRGIFAELAPSDASELKARAEALGRAFIDQGITFSLSGQERPFP
                     LDLVPRVISAAEWSRLERGITQRVKALEMYLDDIYGDQEILNDDIIPRRLITSCEHFH
                     RQAVGIVPPNGVRIHVAGIDLIRDERGNFRVLEDNLRSPSGVSYVMENRRTMARVFPN
                     LFATHRVRAVDDYSSHLLRALRNSAATNEADPTVVVLTPGVANSAYFEHSLLARQMGV
                     ELVEGRDLFCRDNQVYMRTTQGERQVDVIYRRIDDAFLDPLQFRADSVLGVAGLVNAA
                     RAGNVVISSAIGNGVGDDKLVYTYVPTMIEYYLGEKPLLANVETYRCWLDDERAEVLD
                     RIGELVLKPVEGSGGYGIVFGPQASDKELAAVAKKVRDDPRSWIAQPMMELSTVPTQV
                     GSALAPRYVDLRPFAVNDGDKVWVLPGGLSRVALVEGSRVVNSSQGGGSKDTWVLAPR
                     ASSGNRELGAAKVVRSLPKSIPDPALDGSARLTQQQPWPADPQDEQPQQQQQQAMR"
     gene            20005..20265
                     /gene="rpsT"
                     /locus_tag="VFI55_01905"
     CDS             20005..20265
                     /gene="rpsT"
                     /locus_tag="VFI55_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET7072822.1"
                     /translation="MANIKSQQKRNRTNERARLRNKSVKSSLRTAVRAFREAAHTGDK
                     DKAAELLVVTQRKLDKAASKGVIHKNQAANKKSALARALNKV"
     gene            complement(20279..>20720)
                     /locus_tag="VFI55_01910"
     CDS             complement(20279..>20720)
                     /locus_tag="VFI55_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216929.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET7072823.1"
                     /translation="DTGGDVDADAVRRYHSGKAEVKGFDIADKAVAGDVAGATEALRW
                     AMMRGEPLVVLADALAEAIHTIGRVGPLSGDPYRMAAQLGMPPWRVQKAQKQARRWSS
                     DTVATAMKVVATLNANVKGAVADADYALESAVRQVAELVADRSR"
BASE COUNT         3601 a         7010 c         6731 g         3378 t
ORIGIN      
        1 gcctgttgcc tcacgtcaag atgcgcgcga gggcggttcc gttgcctcat gtgattgtgc
       61 gcgcttgggg actcgggccc gggtggcggg ttgaaacttg ctggtggtca ttttgtggag
      121 ctgtgtggtc agggcctgga tctggcgctg ggtggccgcg gggttgatca gagcgtgggt
      181 tcgggcgaga gcggctatgt ggtcgacggt ggtgtcgggg tgattgatgg cgcggtggaa
      241 tggggtggtc gcggtgtcgt gtttcttcgt caccttggcg ccgtcgcgga ccttggatat
      301 cagcttctgc tgtgggtaga agtagttggt caactgtgac tgaagctgcc agatttcgtt
      361 gagtagcaac agttctggag cggtgtcgta gcggtggtag ccgacaactg tgcggaccac
      421 tgcccagttt ttctgctcga cgtggcagcc gtcgttcttg tttgtcggcc gcgcgcgagt
      481 gaaggtgatc ttgcggtttc tgcaccagac gaagagatag acattgatga attcagctcc
      541 gttgtcagag tccaccccca ggatcgggaa cggcatcttg ttggcgatgc tttccaaggc
      601 gctcaggacg catttggcct ctttgctggg cactgagcgg ttctcggtcc aaccagtggc
      661 gacgtcagtg accgtgagcg tgaatgcgaa cgggccgatg gtgtttccgc cgtcgtggga
      721 gaccagatcg atctcaacga agcccggctt agcgtcgtcc cagtctgccc aggtgtgtat
      781 tgggatctga cttctcagca gcgatccggg cttggtggcg cgatgcccct tgagctgacg
      841 tttggctcgt tcacctgcca ggcgacgatc gatggtggcc gccgacatcg acacgaggag
      901 ttctgcggtg tcatcgtcga tgaccagctc gccgaagtgc cgtaacactg ccactagttc
      961 gttcaacatc ggcgccagtc tcttgcctgc cggcatcccg agtaccagcc agcacgccgt
     1021 aagtgcagca atcacatccg gcccgtagac cgggggacgc ggacttcgcc tcggcgctac
     1081 caccttgggc tgcagcgcag ccttgagcgc tttgcgggca tggttacggt gccaaccagt
     1141 gttggcgcac aactcgtcca gaatcctgct cttgactcgc ctatccgcca ccacgtaccg
     1201 gatcgcggtc atctcggtca cagccctgcg ctcggccaac gtcaacccca tcctcagggc
     1261 ctaccgagac ccccagcaat ccctcaggca ggcacgccgc acgcgcgcat tttgaatgag
     1321 gcaacgaata gccactttgc gcgcataaat ggcgattcaa cgcgggtggg tggcgataat
     1381 attcgaacat gagttcgcat agccgagggg ggatctacgc agtcttcgac gcgctcgacg
     1441 ccgacttgga tcgtgcctgc gcgctgtcgt ttgaggagtt gaccacaccg gaacggcttg
     1501 gagcgtctgg aaacggtcgc tcggcgggtg ccggtgccgg cgcaccagct gatcaatcag
     1561 ctcgctgaac aggccccgcc tgaagagctg ggcggcaagc tcccccacgc actggccgac
     1621 cggctacaca tcacccgcag cgaggcccga cggaggatcg ccgaggccgc tgacttaggg
     1681 ccacgccgcg ccctcaccgg cgaggcgctg ccaccgttgt tgaccgcaac cgccgccggc
     1741 cagcgcaccg gacagctcgg gcctggtcac gtgcaggtga tccgccgctt ctaccaccag
     1801 ctgccctgtt cggtggacgt cggaacccgc gaggccgccg aaacgcatct ggccaagttg
     1861 gccaacgggt ttcgtcccga ccagatcgcc ggtttggccg acacgctcac cgactgcctc
     1921 aaccccgacg gcacctacac cgacgaggac cgcgcccggc gacgtggact gaccctgggt
     1981 aagcaaggcc ccgacggcat gtcacggatc agcggttggc tgagccccga agcacgggcg
     2041 accttcgaag ccgtcctggc caagaccgcc gcccccggcc tgtgcaaccc ggaggacgac
     2101 aaccccgtcg tggacggccc tccccccgag gacacggccc ggcgggacac ccgcagcgcc
     2161 acccaacgca accacgatgg actcgatgcg gcgctgcgcg ccgtgctggc gtcgggaaag
     2221 ttggggcagc acaacgggtt gccggccacc attatcgtgt ccaccacgct caaggagctc
     2281 gaatccgcgg ccggtacagc ggtcaccggc ggaggcaccc gactgcccat gaacgacgtg
     2341 atccgcctgg cccgccatgc ccaccactat ctggcaatct tcgacaacgg cagagccatc
     2401 gcgctgtatc acaccaaacg cctggcatcc gccggacaac gaattgtctt gtacgccaag
     2461 gatcgcggct gcacgcggcc ggggtgtgat gtgcgaggct actggtgtga agtgcaccat
     2521 gtcgaggact gggccatcac gcattgcacc gacatcgaca cgctgacact tgcctgcgga
     2581 ggcgatcacc cactggttga acccggcggc tggaccaccc gcaaacgcaa agacggcacc
     2641 accgaatgga ttccaccgcc acacctggac tacgggcaac ccaggaccaa catgttccat
     2701 caccccgaga agctcttggc cgacgaagac gacgaaccgt agcccggcga gtggggcggc
     2761 ttcgccctag atcacgccga cgtcagcgca ttctgatctt cgcccgcgcc aacggcaccg
     2821 tcgccggctg gctcagccag gccaccgaga acgggcggca ctcgggtggc ccggacgtac
     2881 acggtgtcgc cgtcacgcaa cgccagcgcc tcagcgtcgc cccgggtgat ctgggcggtg
     2941 aaagcgccac cggtcgccgc gttggtcaac tccacgcgca cctcgaagcc gagcaccacc
     3001 acccggtcga cggtggcacg caggacaccg gtggactccg cggtgccgtc ggcggtggcg
     3061 accgccatgt cgggattgcg gccgacccgg atgtcgtgcg ggcggaccag ggcgccgttc
     3121 agtgtggaaa ccgcacccag gaacgacatc acgaacgcgt tcgccgggga gtcgtagact
     3181 tcggtcgggg atccgacctg ctcgatgcgg cccttattga gcaccgcgat gcgatcggcc
     3241 acgtccagcg cctcggactg atcgtgggtc accagcaccg tggtgacgtg cacctcgtcg
     3301 tgcaagcggc gcaaccacgc ccgcagatcc tcgcggacct tggcgtccag cgcgccgaac
     3361 ggctcatcga gcagcagcac ctgcggatcg acagcgagcg cgcgcgccag cgccatgcgc
     3421 tgtcgttgac cgccggacag ctggttgggg tagcgagtct gaaaaccgct caagcccacc
     3481 acttcgagca ggttgtcgac cttctgtttg atctcggcct tgggccgctt gcggatcgtc
     3541 aacccgtagg ccacgttgtc gcgaacggtc atgtgcttga acgccgcata gtgctggaac
     3601 acgaatccga tgccgcgacg ctgcggcggc acccgggtca cgtcacggcc attgatcgtc
     3661 acggtgccgg tatcgggctg atcgaggccg gcaatggtac gcaacagcgt cgacttgccc
     3721 gaaccgctgg gacccagcag tgccgtcagc gaaccggtgg gcacgacgaa gtccacatgg
     3781 tccaaggcga cgaaatcgcc gtagtgcttg taagcgtcgc gcaccacgat cgcctgatcg
     3841 ccacgcttgg ttgcgtcatc ggtcatcgac gagtctcctt gtcacgccta ttttgtcgct
     3901 cgtgcccggc gggcgtcgag aactacttgg aagatcagga ccacgacggc aacacacatc
     3961 agcagcgtcg acaacgcata ggcgccgtat tcggccccgc ggttatatcg gtccgagaca
     4021 agcaatgtca gcgtttgcga cacccctgga aggttggatg acaccatgat caccgccccg
     4081 tactcgccga gagtacgcgc gacggtcagc accacgccgt atgtcaatcc ccaccggatg
     4141 gacggcaagg tgatccgcca aaacgtctgc cacgagcccg atcccagcgt tgccgccgcc
     4201 tgttcctggt cggttcccag ctcatgcagc accggttcca cttcgcgcac gacgaatggc
     4261 agggtgacga agatgctggc cagcacaatg ccgggcaggc cgaagatgat cttgaagcca
     4321 aggtctttct cgacaaaacc cagcacaccg cccgatcccc acaacacgat cagcgccacg
     4381 cccacgatga cgggcgagac cgcgaacggc aaatcgatga ccgcttgcag cactcccttg
     4441 ccgcgaaagc ggttgcgcgc gagcgccaac gcggtgggca cgccgaaaat cacgttgaga
     4501 ggcaccacga tggccaccac cagcagcgtc aggttcagcg ccgatatcgc cgccggggtg
     4561 ctcacccagt cgaagaactg gcccagcccc ggctggaagg ttcgccacag gatcagcgtc
     4621 accggaacga ccaacaacac gaagatgtag gccagcgcca caaatcggat gaggtagcgg
     4681 accccgggag aagatgtcac gcggccgact cctcacgctt ggccgcccgc gcgcccacga
     4741 cccggagcag cagcagcacg acaaacgaaa tcgtgagcag cacaatggat atcgcggcgg
     4801 caccagtgcg gtcgtcgttc tcgatcaagg tccggatcca ctgcgaggac acctcggtct
     4861 tgcccggcac cgcaccaccg atcaggacca ccgaaccgaa ctcgccgatc gcacgcgaga
     4921 acgccagccc cgcaccggac aacaacgccg gcaacagcga cggcagcacg accgaggtga
     4981 agatcttcgg tccggtggcg cccagcgacg ccgcggcttc ctcggtttct cgatcgattt
     5041 ccagcagcac cggctgaacc gcgcgcacca cgaacggcag cgtgacgaag gccagggcga
     5101 cccccacgcc ccagggggtg tgctgcaggt gaatacccac cgggctgttg tgcccgtaca
     5161 gggccagcat caccaggctg gcgacgatgg tcggcagcgc gaacggcaga tcgatgatcg
     5221 cgtcgacgag ccgtttgccg acgaagtcgt cacgcaccaa cacccaggcg atcaccaaac
     5281 cgaagaccag gttgaggacc gtcaccgccg aggagatgac gagcgtcacc cggaacgact
     5341 ccaccgccgc gtgggaggtc accgccagcc agaagtcctc ccagccgccc ccggccgcct
     5401 gccagacgat ggcagccagc ggcagcagca cgatcaccga cagccacagc atcgccaccc
     5461 cgaggcgaag cgatgtggcg tccagtccgc gcacacgccg agccgcgggc ggtgccggtg
     5521 ccgcctcgag ctcgggccgg atcgcctcgg ggttggcgct gctgctcaaa aatgacccgg
     5581 tcatccggtg gcctgcgtgt agatcttggt gatgctgccg gtgtttttgt cgaacaactg
     5641 cggatccacc gtggtccacc cgccgaggtc ggcgatggtc cacagtttct ccggcacggg
     5701 aaattggtcc cggaaatccg cggagaccga cggatcgacc ggccggaaac cggcctgcgc
     5761 ccacaacttc tgcgctgcgg ccgtgtactg gaaattcttg aacgcggtgg cggcctgcag
     5821 gtgcgggctg gtgtttacca ccgccagcgg gttttcgatc ttgaacgtct gctgcgggat
     5881 gacgtgttgg accggtttgc ccgcgcgctc cgtggcgatc gcctcgttct cgtaactgat
     5941 caacacgtct ccgctgccct gcacgaaaac atcggtggct tcccgtcccg agccgggacg
     6001 caacttgacg tggtcgccta ccaacttctt gatgaagtca acgccggcct gaccgttgtt
     6061 gccgccttgg ctcttcacgg cgtatggcgc aagcagattc cacttcgctg agccggagct
     6121 caacgggctg ggcgtgatga cctgaatgcc gggccgcaac aggtcatccc aatccttgat
     6181 gtttttggga ttgcccgcgc gcaccaccag cgcgaccacc gaaccgaatg ggatgccctt
     6241 ggtggcgtcg gtattccagt ctttggacac cttgcccgcc ttgaccaacc gggtgatgtc
     6301 cggttcgacc gagaagttca cgagatcggc cggcttgccg tcgacaacgc cgcgtgactg
     6361 gtccccggaa gcgccatagg aggtgatcac ctgcacgctc ttaccctcgt cggacgcatt
     6421 gaacgcggga atcaccttgc tccaaccggg ttctggtgcg gagtaggcga ccagggtgag
     6481 gctggtgtgg gcagcggtca gcccaccgcc gccaacgacg tcgctggcgc cgccctgaca
     6541 cgcagcgacg acaccgacaa tcagggtgag caccccgaca aaccggacgg gccgccagcg
     6601 cggtccgcca cgaatgtcgt tgagcattgg tggcctttcg gtgcgggacg tagggatatc
     6661 ggtcccgtta ccgcggaaag gcgcctagaa cttacggaga attcacgact ccataccaga
     6721 ccgcgcacgg gttcgggagt cagcgacaac agcgcacgag gacacagcat tccagaacac
     6781 cggacgccac gcgcatgccg cgaagcctaa caggaattcg ctgggccgcc accgcacggc
     6841 ccgcagcagc aggtcagcta cgcataccag gcggcgcggc tcagcgtgtc agtagccacg
     6901 cgacgatcac cagcaccacg agcgtgacca gaaccaacgt cacgtgtgac cgaggcatac
     6961 gcgctacccg ggcgccccat cggcgtggcg gacgcggcgc atcaactgga tgcccttgcc
     7021 gagcagacga tccagcaatt cggcagtgat gtaggcgaca gccaacacca cgggcatcac
     7081 caccgtggcc tgcaagacaa acgggagccc ggaaagccac agctcgttgc cgtcccacca
     7141 attcaggaac gcctccatcc cgctcacact attcgccccg gaagccgaaa ccaatgtgcc
     7201 aggatcggcg agtgcccacg gcatccttcg actctgcggc aacgacctgt ctcatcgccg
     7261 gcggcggacc ggcaggcatg atgcttggct tgctgctggc ccgcgccggt gtgcaggtca
     7321 cggtcatgga aaagcacgcc gacttcctgc gtgacttccg gggagacacg gtgcacgcga
     7381 gcaccttggg gctgcttgac gaactcggat tggggccgcg gttcgcgcag cttccgcatc
     7441 ggaacatcga caccgtccgg atggagatcc agggcgatcc ggtgaatctg gacctgcgcc
     7501 ggctgcccgg cgctcatcaa tacatcgcgc tggtgcccca gtgggacttt ctggagatga
     7561 ttgccggcgc tgccgaggcg gagccgacat ttcggctgtt acgcagcacc gaggtgactg
     7621 gagtgattcg cgacggccgc cgcgtactgg gcgtcaccta ccgtgaccag acgggcgaag
     7681 ccaaggagat gcgcgccacg ctcacggtgg cctgcgatgg ccgaacgtcg acggtgcgtt
     7741 cggcagtggg cctggtacca cgcagcttcg gcgccccgat ggacgtctgg tggttccggt
     7801 tgccgcgccg gcccgacgat ccgtctgggc ttgccggcgt gctccgctcg gggcacgcgt
     7861 cgatcatgat tgaccgcggg gactactacc agatcgccta catcattccc aagggagccg
     7921 acgcgcagat gcgtgcccaa ggcgtcgagg cgttgaagag cgcgctggtg ggcatggtgc
     7981 cctggatcgc cgaccgcgtc gacacgttga cgtccttcga cgacgtgaaa ctgcttgacg
     8041 tggaacttaa ccggctgcgt cggtggtatt cggacggagt gctgtgcatc ggcgacgcgg
     8101 cgcacgcgat gtcgccggtc ggcggcatcg gcatcaacct ggcggtggcc gacgcggtgg
     8161 ccgcggcgcg catactggcc ggtccgttgc gcgcgggacg cgtgtccacc ggccggctcg
     8221 ctcgggtgca ggcacgtcgg tggctcccgg cagcgctcct gcaggcggca caacggtcga
     8281 tccatgccaa cgtcatcgcc gccgctgtgg ccggcggtga tcgaccaccc ccgcgggccg
     8341 tgcgggccgt gagccgatcg ccggcgcttc gacgattcgc cggctacctg gtggcgatcg
     8401 gcccgctgcc cgagcacgcc ccgcggtacg ccaggcgagc cgggtagtcc ggccagtgga
     8461 ggccgagcaa ggccattgca ggccgagcag gccgacacct gggtcaaaga atggacgccg
     8521 ggcggccaga gagtcgatga tgtcgggatg gttctgcacg cccaacccgc cgatcaaccc
     8581 gccttccaac ccgccgacac cggtcccgag gaagcctttg aatcggcccc cagaatgacc
     8641 tccgccccgc cgctggaatt cagctccgcc gggccgctgg cgaccagcgc gtcacttcgg
     8701 aacccgtttc cgccgatcgc cgattatgcg ttcctgtcgg actgggagac gacatgcctg
     8761 atctcccccg ccgggtcggt ggaatggctc tgcctgcccc ggcccgactc gccgagcgtg
     8821 ttcggggcga tcctggaccg tagcgcgggc catttccggc tcggcccgta cggcgtctcg
     8881 gtgccctcgg cccgccgcta ccttcccggc agcctgatca tggagaccac ctggcagacc
     8941 ccgaccggct ggctgatcgt gcgtgacgcg ctcgtaatgg ggccgtggca cgacatcgag
     9001 cggcgatcgc ggacgcatcg ccgcaccccg atggactggg acgccgaaca catcctgctg
     9061 cgcacggtgc gctgcgtcag cggcaccgtc gaggtgatga tgagctgcga gcccgcgttc
     9121 gactaccacc gcgtcggggc ggcctgggaa tactcggcca acgcctacgg cgaggccatc
     9181 gcccgggcca gcaggcaacc ggacgcccat ccgacgctgc ggctgaccac caacctgcgg
     9241 atcgggctgg agggccgcga agcgcgggca cgcactcgga tgcggggggg cgacgacgtg
     9301 ttcgtcgccc tgagctggac caagcatccg gcgccgcaga cctacgaaga ggccgccgac
     9361 aagatgtggc aaaccaccga gtgctggcgg cagtggatca acatcggcaa tttccccgac
     9421 cacccgtggc gggcctacct gcagcgcagc gcgctcaccc tgaaggggct gacatattcc
     9481 ccgacgggtg cgctgctggc cgccagcacc acgtcgcttc cggaaacacc gcagggcgaa
     9541 cgcaactggg actaccgcta cgcctgggtt cgcgattcga cgttcgcgtt gtgggggctt
     9601 tacacgctgg gactcgatcg tgaggccgac gatttcttcg ccttcatcgc tgacgtttcc
     9661 ggcgccaaca gcaacgagcg ccacccgctg caggtgatgt acggcgtggg cggtgagcgc
     9721 agtctggtcg aagacgagct gaaccatctg tccggctacg accacgcgcg cccggtgcgg
     9781 atcggcaacg gcgcctacga ccaggtgcaa cacgacatct ggggctcgat tctggactcg
     9841 ttctacctgc acgccaagtc gcgcgagcaa atcccggaga cgttgtggcc ggtgctgaag
     9901 aagcaggtgg aagaggccat caagcactgg cgcgagcccg accgcggaat ctgggaggtg
     9961 cgcggggagc cgcaacactt cacgtcgtcg aaggtcatgt gctgggtggc gctggaccgc
    10021 gggtccaagc tggccgagcg gcagggtgag aagagctacg cccagcagtg gcgcgccatc
    10081 gccgaggaca tcaaggccga catcctggaa cacggggtgg attcccgcgg tgtgttcacc
    10141 cagcgctacg gcagcgacgc tctggatgcc tcgctgttgc tggtggtgct cacccggttc
    10201 ctgccgccgg acgacccgcg ggtgcgcaac accgtgctgg cgatcgccaa tgaactcacc
    10261 gaggaaggcc tggtgttgcg ataccgggtc gaggagaccg acgatgggct gtccggcgag
    10321 gagggcacct tcacgatctg ctcgttctgg ctggtgtccg cactggtcga gatcggcgag
    10381 gtgacccgcg ccaagcggct gtgcgagcgg ctgctgtcct acgcaagccc gctgcacctg
    10441 tacgccgagg agatcgagcc gcgcaccgga cgccacctgg gcaacttccc gcaggcgttc
    10501 acccatctgg cgctgatcaa cgcggtggtc cacgtgatcc gggccgagga ggaggccgac
    10561 ggctccggga cgttccagcc cgccaacgcc ccggtgtgag aggccgggtc agcgcagggc
    10621 gctgttagac gcgtcagctc agggcgttga ccttgtccat catggccttc gcgatgtcgg
    10681 tggcgctggt gtcgacgttg gccgatccgg gatctgagga ccgccgctcg ccgaagaaca
    10741 ccacctcgac ctcgacgaga cagttcaact ggacgccgat ggcacgggcc tgcggcgtgg
    10801 gccgcagggc cggcatgttg ggcagcaccg ccgtcgcggt gttggtggcc gcaagcacgg
    10861 aaccggcaac gcggacgtcg ttgatatcgg tcgtcgtgct gatcgggccg ctttgttccg
    10921 tcatcgtcgt gcccttgcac tgctgccact gcgccgagaa cgtggcgaac agcacctggg
    10981 cctgctccgc cgaaggcagc gagaccacgc cctcttggac gccgatcact tgcgccggct
    11041 cgccgttgtt ccaccacgac tccgacgcga cgtccttgac gtcagcggac tggtagacgc
    11101 tttgctgcag catggtcgtc acgcccaggc aaccgggtga cgccaccggt ccgtcacggt
    11161 gggtgagctt gtccgggccc ccgaactcgg ccggcctacg ggagacaaaa ggctggctga
    11221 gcatttgcga cagcgtggtg ccgtcaagga gcacctgctg gacggttgcg ccgctaagcg
    11281 gccgcggctt caggttcggc gccggtttcg ccgtgccatc caccaccgat ccacagcccg
    11341 ccaccagcgc ggccatcacc gtcagcgccg cggccggtcc aagtatccgc atggctactt
    11401 tttacctttg tcccccgcgg cgtcgctcga cagcgcggcg acgaacgcct cctgcggcac
    11461 ctcgacgcgc ccgatggtct tcatccgttt tttgccttcc ttctgctttt ccagaagttt
    11521 gcgtttacgg gtgatgtcgc cgccgtagca cttggacagc acgtccttac gaatcgcgcg
    11581 aatgttctcg cgcgcaatga ttttcgatcc aatggccgcc tgcaccggca cctcgaactg
    11641 ctggcgcggg atgagttcct tgagcttggt ggtcatcttg ttgccatacg ccgaagcccc
    11701 gtccttgtgg acgatcgcgc tgaacgcgtc gaccgcctcg ccctgcagca ggatgtcgac
    11761 cttgaccagc tgggcctcct gctcgccggc ctcctcgtag tcgaggctgg cgtaaccgcg
    11821 ggtgcgcgac ttcagtgagt cgaagaagtc gaagatgatc tcccccaggg gcatcgtgta
    11881 gcgcagctcc acccgctcgg gcgacaggta gtccatgccg cccagctcgc cacggcgcga
    11941 ctggcagagc tccatgatgg tgccgatgaa ctcgctcggc gcgatgatgg tggtcttcac
    12001 caccggctcg taaaccgtgc gaattttgcc ctccggccaa tccgacgggt tggtgacgac
    12061 aatggagtcg tccgcgcctc ctgcccgtcc ttctaggatc acccggtaca cgacgttcgg
    12121 cgacgtcgag atgaggtcca ggtcgaattc gcgctccagc cgctcgcggg tgatctccat
    12181 gtgcagcagt cccaggaagc cgcatcggaa cccgaacccc agcgccaccg acgtttccgg
    12241 ctcgtaggtc aaggcggcgt cgttgagctg cagcttgtcc agggcgtcac gcagatcggg
    12301 gtaatccgag ccgtcgacgg gatacagccc cgagtagacc atcggcttgg gttcgcgata
    12361 cccggtcagc gcctctttgg ccccatggcg ggcggtcgtc acggtgtcac cgaccttgga
    12421 ctgacggacg tccttgaccc cggtgatcag gtagcccacc tcccccacgc ccaggccctc
    12481 gctgggcttc ggatcgggtg agacgatgcc gacctccagc agttcgtggg tggcgccggt
    12541 ggacatcatc aagatgcgct cgcgcggggt gatcttgccg tccaccaccc ggacgtaggt
    12601 caccacgccg cggtagatgt cgtagacgga gtcgaagatc atcgcgcggg ttggcgcatc
    12661 ggcgtcgccc tgcgggggcg gcacctcacg caccacatgg tcgagcaggt cggccacacc
    12721 ctcccccgtt ttgccggaca cgcgcagcac gtcactgggt tcgcagccga tgatgtgggc
    12781 tatctcaccg gcgtagcggt ccggatcggc ggcgggcagg tcgatcttgt tgagcaccgg
    12841 gatgatgtgc aaatcccggt ccagcgccag gtagaggttc gccagcgtct gcgcctcgat
    12901 gccctgggcc gcatccacca gcagcaccgc gccctcgcag gcctccagcg cgcgcgacac
    12961 ctcgtaggtg aagtcgacgt ggcccggggt gtcgatgagg tgcagcacat agtcttttcc
    13021 agcatcctca ccggccgata cccgccaggg cagccgcacg ttctgcgcct tgatggtgat
    13081 gccgcgctcc cgctcgatgt ccatccggtc caggtactgg gcgcgcatcg agcgctcgtc
    13141 gacgacgccg gtgaactgca gcatccggtc ggccagcgtg gacttgccgt ggtcgatgtg
    13201 agcgatgatg cagaaattcc gaatctgcgc cggcgcggtg aaggtcttgt cggcgaaact
    13261 gctgatggga atctcctggg ctccagttgt tgaaggcatc cgacgcaggt gagcggcgcc
    13321 tagctggtgc gtccagcgta tccatccggg gtggccggga cacacttgga cacaatctca
    13381 gcggccgctc gcagctatgc tgcgaatatg gcgtctgccc ggaaatctca gtggaggacg
    13441 tttcagcgct tcgcggaaaa cctggttttc aacggtgctc cgcggctggt ccggcacgtg
    13501 cagacctccc agatcgtgct gcgcgagctg cagcaggccg tgaagatcac cgcgaacgtc
    13561 atcgccgcgg tcgcaccgcc gccgcccgca tcgatcacga cgggccggcc ggtgacgagc
    13621 accagcttcc ccaccgccca gcgagcccgc aggctggtgt acgccccgga cctcgacggc
    13681 cgggcggatc ccggcgaggt cgtctggacg tgggtggtct acgaggacga ccccacccag
    13741 ggcaaggatc gacccgtcct ggtcgtgggc cgtgaccgcg ccgtcttgct gggattgatg
    13801 atgtccagcc aggagcgcca cgccgccgac cccgactggg tccgcatcgg ctccggcgcc
    13861 tgggattacg agggcaggga gagttgggtg cggctggacc gggtcctcga tgtgcccgag
    13921 gagggcattc gccgcgaggg cgcgatactc gatcgcgaga tcttcgacgt ggtggccgcc
    13981 cggttgcgcg ccaactattc ctggagttag ccgtcctggg caatcagccc tgccccgggc
    14041 actcagccac gcccgccgac gatgcgggcc gcgcagccgc cagaggtgga gccgggcaat
    14101 cagccctgcg tgatgtagga gtgcagctgc tgctgctgcg cctcgagttc ttccatccgg
    14161 gtcttcacca catcgccgat gctgacgatg ccgatcagct tcttgccgtc caggacgggg
    14221 acgtggcgta cccggttttt ggtcatcaag acgctgatgg cgtcgacagt gtcggacttc
    14281 gtgcacgtgg cgacggcgga cgtcatgatc ttggagatcg ggcgggacag gacgctgggg
    14341 ccgtgtttgt gcagctggcg cacgacgtca cgctcggaca cgatcccgac gaccccctcg
    14401 gcgccgacca ccaccatggc accgatgttc tgctcggcga gaccggcgag cagttcctgg
    14461 acggttgcgt ccgggttgat cgtgacgacc gccgcaccct tgttccgcag aacgtccgca
    14521 atccgcataa aggcctccag ctggttgtga gctgcttcac accaggctaa ggctaacttg
    14581 cccggcggga aagccaccgt cgaccacgag tgtccgtacc ctgccgcccg ccgtctcgac
    14641 gcgcccgcga ttcgtcgcgt cggcgttccc cgaccgcctg acggcgagcc ggatcgccaa
    14701 atgtcactgc ccgccgacgc ggcttgcaag gatagggcca tgatcgagat cactttgctg
    14761 ggcaccggaa gtcccatccc ggatccgaac cgcgccggcc cgtccacgct ggtgcgggtc
    14821 agcgggcagg tcttcttggt ggattgcggg cgtggagtgc tacagcgcgc ggcggcagtc
    14881 ggcgtcggcg ccgcaggctt gtcggccctg ctgctcaccc acttgcacag cgaccacatc
    14941 ggcgacctcg gcgaccttct gatcacgcgg tggatcagca ctttcgcgcc tgatcccacg
    15001 ccgctgccga tcatcggacc gcccggcacg gcggagacgg tggaggcgac gctgaaggcg
    15061 ttcggtcatg acatcggtta ccggatggct catcatgccg atctgaatgc gccgccggcg
    15121 gtcgaggtgt tcgaatgcac cgagggtccg gcgtgggacc tcgacggtgt gtcgatccgg
    15181 gtggccccca ccgatcaccg accggtggca ccgaccatcg ggttccgcat cgaatccggg
    15241 acgacggcgg tggtgctggc cggcgacacg gtgccctgcg ccggcctcga cgacctggcg
    15301 accggcgccg acgcgttggt gcacaccgtg atccgcaagg acattgtcgc caacatcccg
    15361 caacaacgcc tcagggacat ctgcgactac cactcttcgg tcgagcaggc ggcggcgacc
    15421 gcggcccgtg cgggtgtggg cacgctggtg atgacgcatt atgtcccggc gctggtgccc
    15481 ggccaggagg aacagtggcg cgcgctggcc gcctccgaat tcggcgggcg cgtcgagctc
    15541 ggcgacgacc tgcaccgcgt cgaggtcaac gcgcgggcct gaccgtcatt ccctgaccgc
    15601 ggcgcggacg gatggcgcct cccgtgaccg aacatcgctg cgccgcaatg gcaagagcgc
    15661 accgacggcc accagccaga ccagcgccga gacccgcgcg atcggcagga tcaccgccag
    15721 cgggggccag atcagcacca gcgtcgtcaa ttcggccagc gcggcaatcg ccacacccat
    15781 ccaggccacc ggtcgagggg tcaacccgag gacgaggctg ggaaccgcca tgccggcaac
    15841 cagcaggccc aacgccacga tgtgcccggg gccgccgacc aggaacacca ggaagtagag
    15901 ggcccggacc aacgcggcgt ccgcgctgac ctccggccgt gacaacgtcc atgccaacaa
    15961 acccgtcagg cccagcgcgc cggccgccag ggtgccgccc gtgagcgcga tcgtgccgcc
    16021 cggcgccgtg acgccgagct gccgcaaacg ggcactggcc gtggccgcat agagcgcaag
    16081 cgggaccgac gacgcgaacg ccgccgtcgc gatcacctgc acggcgaccg gctgggcgcg
    16141 gacgtacgcc tgaaccgctg cggccgggcc atagggcaac ggcatcaccc cgccgagggc
    16201 gaccccgatg gcgagcccac cgaacagcag gaccaaacag accgcggaga gcaataccaa
    16261 cggcggtccg ccctgccggg cgcgaccgcg acccgggcca gttccatccg tgcgcgcgtt
    16321 catggtctga acgatattcc cgcgctcgca cgaggtggct aagccagccg ggtgatctcc
    16381 acaatcacgt cgagatcggt ggccccctgc ccggagaaaa tgcctttcag cggggagacg
    16441 tcggtgtaat cccggccggc ccccacgctg acgtattgct cggtgatctc cgcgtcgttg
    16501 gtggggtcgt agttccacca gtcgccggtc caggcctgaa tccaggcgtg gctgcgacct
    16561 tccaccgtgt ccccgacgac ggcgttgggt ttggggtgca gatagccgga cacgtagcgc
    16621 cccggaattc ccatgctgcg caacacaatc aacgacaaat gcacgaaatc ctggcagaca
    16681 cccttgccct cactgagggc gtccagtccg gaggaatgca cgccggtggt tcccgggagg
    16741 tagtccagct cacggcgcac ccagtcgccc accgcgacga cggcgtcccg ggggccgtag
    16801 gccttggcga ttcttttggc cacggacgcg acacgcttgt tgctgtttgg ggtgtgcgcg
    16861 gtggggcgaa gcaactcgtc gtaacggtcg atcaccgcct cggattccag gtcgtcccag
    16921 ctgacttttg ccgccggcgg ctccggccgc tccgtctcga cgaccgacga cgacgtcacc
    16981 gtgagatcgg tgtgcggcgc gtgcaggtcg aacgccgtca ccgccgtgcc ccaatagtcg
    17041 atatagcggt aggagcgggt cgcggggatg gtttcgatgc ggttgaggac gacgttctgc
    17101 ctggtgtccg accgcggggt caaccggacc tcgttgtagg aggcggtcac cggcgattgg
    17161 taggcgtacc cggtggtgtg caccacccgc agccgccaca tcaggcttcg ccctgtcggg
    17221 cgaccaaccg gccgcgctgg ccggcatccg accacgccac ccagggcgtc acgtggaagt
    17281 actgcagcga caacgcttct ccgacgtcgt agcacgttcg ctgcaagtcc gccaggcggc
    17341 tctccagtga ctcgagcaac acgccgggtt ccacgaactc cagttcgctg cgagcccgcc
    17401 cgagcagccg ctgcgcctcg gcggtggccc cgatccggct ctgcggatcg tgcgccagct
    17461 cgtggaggtt gtgttcggcc atcctcagcg aataaaacac cgaacgcgga aacaggcggt
    17521 cgaggagcat gaattcgacc acccggttgg catccagcac accacggtag gtgcgcagat
    17581 aggtgtcgtg cgcacccgcc gagcgcaaca gcgtcaccca cgccggcgac gacgcgctgt
    17641 ccccgaaccg cgacaacaac aggcgcaccg tcatgtcgac ccgctcgatc gcgcgcccca
    17701 gcgacatgaa gcggtagccg tcgtcgcgcg acagcgtcga gtcggccaag ccggcgaaca
    17761 tcgccgcacg gccttcgacg tacgacagaa actcgtgcgg gccaaggcgt ttcgcggcac
    17821 gctcgcgttc ggccagggcg tggtaggtgg tgttgaggca ctcccagatc tcgctggatg
    17881 tcacttcccg ggccgacttc gcgttttccc gtgccgctgt gatggcgtcg acgatggagc
    17941 agccgcccga cacgttggta ctgaaggcca ccaggtcggt cagcgaccag acgtccaggt
    18001 cgtgatccgg cgggtcgatg ccgagcaccc ggagcagcag ccgggatgcc tggtcggggt
    18061 cgacgctgga atcctcgagc aactggtgca gcgccacgtc cagaattcgt gcggtgtcat
    18121 cggcgcgctc gacgtagcgg ccgatccaat agagcgcctc agcgttgcgg gccagcatca
    18181 gcgcatcgcc tgctgttgct gctgctgggg ctgctcgtcc tgcggatcgg ccggccacgg
    18241 ctgttgttgc gtcagccgcg ccgagccgtc gagcgccggg tccggtatgg acttcggcag
    18301 cgaccgcacc accttggccg cgcccagctc acggttgccg ctcgacgcgc gcggtgccaa
    18361 cacccaggtg tccttcgagc caccgccctg gctggagttg accacccgcg agccctcgac
    18421 gagggccacc cggctcagcc cgccgggcaa cacccacact ttgtcgccat cgttgaccgc
    18481 gaacggccgc agatcgacgt agcggggcgc caacgcgctt ccgacctgcg tcggcacggt
    18541 cgacagttcc atcatcggct gcgcgatcca gctgcgcgga tcgtcgcgga ccttcttggc
    18601 gacggccgcc aactccttgt cggaagcctg cggaccgaac acgatgccgt aaccgccgga
    18661 cccctcgacc ggcttgagga ccaattcgcc gatccggtcc agcacctctg cgcgttcgtc
    18721 atccagccag caccggtagg tctccacgtt ggccaacagc ggtttctcac ccaggtagta
    18781 ctcgatcatg gtcggcacat aggtgtagac gagcttgtcg tcgcccacgc cgttgccgat
    18841 cgcgctggaa atcacgacgt tgccggcgcg ggcggcgttg accagaccgg ccacgcccag
    18901 cactgagtcg gcacggaact gcagcgggtc caggaaggca tcgtcgatac gccggtagat
    18961 gacgtcgacc tggcgctcgc cctgggtggt ccgcatgtac acctggttgt cgcgacaaaa
    19021 caagtcgcgg ccttcgacga gctcaacgcc catctggcgg gccagcagcg aatgctcgaa
    19081 gtacgccgag ttcgccacgc ccggggtcag gacgacgacg gtcgggtcgg cctcgttggt
    19141 cgccgcggag ttccgcagcg cccgcagcag gtgcgaggag tagtcgtcga ccgcgcgtac
    19201 ccgatgggtg gcgaacaggt tcgggaagac ccgcgccatg gtccgccggt tctccatgac
    19261 atacgacaca cccgacggcg agcgcagatt gtcctcgaga acgcggaaat tgccccgttc
    19321 gtcgcggatc aagtcgatgc cggcgacgtg gatccgtacc ccgttgggcg ggacgatccc
    19381 caccgcctgg cggtgaaaat gctcgcagga cgtgatcagg cggcgcggga tgatgtcgtc
    19441 gttcaggatc tcctgatcgc cgtagatgtc gtcgagatac atctcgagcg ccttgacccg
    19501 ctgggtgatg ccgcgctcca gccggctcca ctcggcggcc gagatcaccc gtggcaccaa
    19561 gtcgagcgga aagggccgct cctgacccga cagcgagaac gtgatgccct ggtcgatgaa
    19621 tgcccggccc agcgcctcgg cgcgggcctt gagctctgag gcgtccgagg gcgcaagctc
    19681 ggcgaagatg ccgcggtagg ggccgcggac gttgccctgc gcgtcgaaca tctcatcgaa
    19741 ggccgccgaa tacgagtccg acgtgttgta tccgccgaaa atgcgctcag agcgcgcgtt
    19801 gggacgccca gcaccgccgc gcgaccctcg cctgttttcc tcaagctgac ccggaagact
    19861 cactttagag atggtgcctc aatcgatgtt ccggcaggct gcgggttccc tttttgggag
    19921 aaaacgtaac cgactgctaa cctgagcagt cgttattggg tgggtcgccc aagcaaatac
    19981 cgattcgaag aaggaacaac aggcgtggcc aacatcaagt cgcagcaaaa gcgcaaccgt
    20041 acaaacgagc gcgcccgact gcgcaacaag tcggtgaagt cgtcgcttcg caccgccgtg
    20101 cgtgcgttcc gcgaggcagc ccacaccggc gacaaggaca aggccgcgga gttgctggtg
    20161 gtgacccaac gcaagttgga caaggcggcc agtaaaggcg tgatccacaa gaaccaggcg
    20221 gccaacaaga agtcggcact ggcgcgggcc ctcaacaagg tctgacccgc cggcgttctc
    20281 agcggctccg gtccgccacc agctccgcaa cctgccgtac tgcggattcc agcgcgtagt
    20341 ccgcgtcggc gacagccccc ttcacattgg cgttgagcgt cgccaccacc ttcatcgcgg
    20401 ttgccactgt gtcactcgac cagcggcggg cttgtttctg ggctttctgc acccgccacg
    20461 gcggcatccc cagttgcgcc gccattcgat aagggtcgcc ggacagcggc cccacccggc
    20521 cgatggtgtg tattgcctcg gccagcgcgt cggcgagcac caccagcggt tcaccgcgca
    20581 tcatcgccca ccgcagcgcc tcggtggcac ccgccacgtc gccagcgacg gctttgtccg
    20641 cgatgtcgaa acccttcacc tcggccttcc cgctgtgata gcgccgcacc gcgtcggcgt
    20701 cgacgtcgcc gccggtgtcg
//
LOCUS       DASSGQ010000041         9162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1309079,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000041 DASSGQ010000000
VERSION     DASSGQ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9162
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309079"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(787..1014)
                     /locus_tag="VFI55_01915"
     CDS             complement(787..1014)
                     /locus_tag="VFI55_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET7072824.1"
                     /translation="MTTGLLNPTHIILLLLVALIILGPKRLPQAGRSLGRGMREFRDG
                     ISGRDDPPEQTLPPESADDSPVARHSPGQGE"
     gene            complement(1083..2951)
                     /locus_tag="VFI55_01920"
     CDS             complement(1083..2951)
                     /locus_tag="VFI55_01920"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET7072825.1"
                     /translation="MLTRRQFIKRGAAGAGGIMVGNVLLQAGAATAVAPTANLTSYMM
                     PFAVPPAVALGAGDIHAFTAKEITRQLHPQLAGATHPWSYDDGSSAYSGYLGPTIVVH
                     SGSAATLTYSNGLPATYPFPRIPVDRNLTDGDNRTRIIGHLHGAFDSGLNDGNPAASS
                     EGGVLFGDTQSAFYPNNQRATLLWYHDHATGATRLNVFAGLAGGYVIRDAFDTGGPKN
                     PGDPNPWMPFGYGIGAGKYEIPLVIQDRQFNAANGDFLYPTMPPGAGTTGECGDGLNV
                     GGYPNTVDPWPNSTGPWIGEYFGDEMLVNGVCSPVLDVEPAIYRFRILNGCNARFLNL
                     TFQNVGPGVAPPPTFVQIGAEQGLFKTPVTLGSLPMVNAERADVIVDFRRFAGQELLL
                     RNANMPKPYATPAPRLQDIMKFRVSLPSTQANNAPLAAVPTGAIDGGEVAGMGLGSAV
                     LTRQIVLQEWNAGLPGWHLTLSPYSVDQATLPAVAPITTPDQAACFHTSRSDILQKGF
                     VPEEPKSGTVEEWEFYNYTADTHPMHMHLTRFQLVDRRPIGTRAGGAGTVAPAPWEQG
                     WKDTVAAHPGMVTRIRQKFDLPNGTIPNTADATYVYHCHIVEHEDNDMMRPFIVVA"
     gene            complement(4389..8915)
                     /locus_tag="VFI55_01925"
     CDS             complement(4389..8915)
                     /locus_tag="VFI55_01925"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxKF domain-containing protein"
                     /protein_id="HET7072826.1"
                     /translation="MLSRSKTGARAASGLWRRAAILAALVSFTLASVLAMSAAGIAST
                     VSSANFTGGSGTVSVGSVLYAKQGGALTLTVNTSSDTKCVDVTGAFTGHQTSSSAKSS
                     WTFNFTASTGDGVKTVTATASPNFNANNCTGQSQSPRSASFILDNTGPVVTAALSPTP
                     NGAGWNDSNVSVTWSAADAGSGVASGPTPATDSQTSNTAGVTKTSTATDRLGNSGSGS
                     VTIKLDKTAPTITGNRSPAANANGWNNSDVTVGFSCSDSLAGIKSCSGPTTLTDSAAN
                     QSVTATAVDNADNSASASVSGINIDKVAPTLTGAPTTSPNDAGWYNGNVTIHWSASDA
                     LSGLAGSSPTDSTISSEGTGLTAQASVFDKAGNTTNAPSSPAVKIDKTAPLTTATAPT
                     NWNNTDVTVSLDAADALSGVADTFYELDGGPQQSGASVQISAEGTHSLQFWSVDKAGN
                     TEAAKTVNVKIDKTPPTITDVFDPLPNANGWLQSDVTVTFACADPGSSGIAGSGIASC
                     TGPQTVSTEGKDQAVTGTAIDNAGNATTDPAKVSIDKTAPTIKASVDRPANAAGWYMD
                     DVVVSFECADALSGIDTCPVPKTLGEGADQSTSGTATDAAGNSASDGVSHINVDKSAP
                     TLAGAATTAPNANGWYRDDVTVHWTCSDGLSGVDGGCPADSVISGESDNLSASAVVSD
                     KAGNTTATAVDHIKIDRTPPTTTVSVTDPLDSGWYANAAKVTLTGHDGLSGVDKTYYA
                     VDSGAAQAYSGPFDFALKGVHTISFWSVDDAGNTEDQTAAGHTVTLKIDNVRPTITGA
                     RTPAANQNGWNNQPVTVSFSCADQESGVAGCSDPTTVSTEGAGQFVTGNAADNAGNTA
                     ETMVTGINIDLTPPDIAGQLPDPNGIDSTGAKWYTGDVAVNWACADGLSGIDGPCPAA
                     GTISGEGRHLGAGPVSVSDKAGNSSFASVTDVNIDRNGPVISGAPTTQPNQDGWYSGL
                     VKVAFGCADPNLADGSPGSGVASCPSDIAITDEGANLSVTSDAAKDYAGNSTAGKTVG
                     GINIDNTPPVSTDTVNCTLVGNYCNAGSPVSMTINASDPTPTGVTGVSGVKEIHYSKD
                     GGATWMTATGSSVNVPITLSASGTAAAYYYAVDKAGNAEDKHADSINYDGTPPAVSHT
                     LAPLANAADWSNANTLVHFNATDDSGGSGVNTGTLTPDQTYSTETADQLITGSADDNA
                     GNHGTDSFHFHLDKTAPTISAAVTPGSPDGNNGWYVHAVLVTFSCADPAAANGAAGSG
                     VATCPDPVTLSQNGANQSVTRSVADKADNTASKTASGINIDMENPTLTIGGVKDGGIY
                     TLGSVPTPTCNATDSFSGVASCKGSLSGGLPNGVGTFTYTATATDKAGNLVTQSVSYK
                     VIYNVPANVAFFLQPINDTAHTASTTLSIFKAGQTVPVKFQLKNAAGQIVQANSAPIW
                     QTPAQGNLTTSAVNEDSFTTTGDSASGFRWDSTAQQYIYNWNTSSTQGGYYWKIGVKL
                     DDGMTYFTDIGLRK"
BASE COUNT         1356 a         2997 c         3096 g         1713 t
ORIGIN      
        1 acggctcgcg gcccggatcg gcgggaaccg cgcttgcctc gcgctctcgc gcaagctcct
       61 caaacgcagc taccacatcc tcaaagacct cggcgaccaa gcactcgctc cggtcgcctg
      121 accgctgtcg cgcggcaagc ccgagatcaa ccgatgaaac cgcggccagc tcctggcgag
      181 actgctgccg acccacctca acgtggggac ggcccagaaa gaatgagcgg ccgcaacctc
      241 tgccggaaca ccccatcaca catcatgtcg ccgacccgga gccacaccgg gccgagtgcc
      301 gaagtaagcc tgggcgtccg cggacacaca accccacaac gcgctcacgc accccccttc
      361 ccgccggcgt tccaccgcca tccatgccgg cgggcgaccc ctcacgcaca caaccaccat
      421 cccgcagaag ccccgccaag aacatcacgc gcttgacaag gccgccgcgg gacaagtaag
      481 cagcagcccg ccgtgtgacc ggcgcgaccg tcgtcctgct gtcgagtcgc atggatagca
      541 gcactctcct ttagccaccg ctcgcgactc cgtcccgcgg gactagcgcg ccggccaagc
      601 gggcgctgac tgttgcttag ccgagccacc ggttcgagga cgctgccgct tgcggtggag
      661 agcggcaacc tggctggacg ctggtggcga tccacgatcg cgccggcttc cgaaatgccg
      721 ggcggatgca gcggtcccac gcgtctgttg acgccgatgc tgctagccga atgcggcagt
      781 ccgctcctat tcgccttgtc ccgggctatg ccgagcgacg ggactgtcgt ccgcggactc
      841 aggtggcagc gtctgctcgg gagggtcatc gcgtccgctg atgccgtccc ggaactcccg
      901 catgccgcgt ccgagggagc gtcctgcctg cggtagtcgt ttgggtccga ggatgatcag
      961 cgcgacgagc agcagcagga tgatgtgggt tgggttgaga agtccggtgg tcatgtgtct
     1021 tgtcctttgc ttcccgacgg agcgccgagc gtgtccggcg ccccgtggcg cggctagtgg
     1081 gctcaggcga cgacgatgaa tggacgcatc atgtcgttgt cctcgtgctc gacgatgtgg
     1141 cagtggtaga cgtacgtcgc gtcggcggta ttggggatcg ttccgttggg gaggtcgaac
     1201 ttctggcgga tccgggtgac cattcccggg tgtgctgcca cggtgtcctt ccagccttgc
     1261 tcccacggtg cgggtgcgac cgtccccgct ccgccggcgc gcgttccgat gggtcgccgg
     1321 tcgaccaatt ggaaccgcgt caggtgcatg tgcatcgggt gcgtgtcggc ggtgtagttg
     1381 tagaactccc actcctcgac cgtgcctgat ttcggctcct cgggaacgaa gcccttctgc
     1441 aggatgtccg atcgcgaagt gtggaagcac gccgcctgat ccggggtcgt aatcggcgcc
     1501 acggcaggca gcgtcgcctg gtcgacgctg tacgggctga gcgtcaggtg ccacccaggc
     1561 aggcccgcat tccactcctg cagcacgatc tgccttgtca gcaccgccga gccaagaccc
     1621 atccctgcga cctcgccgcc gtcgatcgct ccggtgggga ccgccgcgag gggcgcatta
     1681 ttcgcctgag tcgacggcag gctgacgcgg aacttcatga tgtcctgcag ccgtggcgca
     1741 ggggtcgcgt acggcttcgg catgttcgcg ttgcgcaaaa gcaattcctg accggcgaac
     1801 cttctgaagt cgacgatcac gtcggcccgc tcggcgttca ccatcggcag cgaccccagc
     1861 gtcaccgggg tcttgaacaa gccctgctcg gctccgatct ggacaaaggt cggcggcggc
     1921 gccacgccgg gaccgacgtt ctggaaggtc aagttcaaaa accgggcgtt gcagccgttc
     1981 aggatccgga accggtagat cgcgggctcg acgtcgagca ccggcgagca gaccccattg
     2041 acgagcatct cgtcgccgaa gtactcgccg atccacgggc cggtgctgtt cggccacggg
     2101 tcgaccgtgt tcggatagcc acccacattg agcccgtcac cgcactcgcc ggtcgtgccg
     2161 gcaccgggcg gcatcgtcgg atacaggaaa tcgccgttgg cagcattgaa ctggcgatcc
     2221 tggatgacga gcgggatctc gtacttgccc gctccgatcc cgtagccgaa cggcatccac
     2281 gggttagggt cgcccgggtt cttcggtccg ccggtgtcga acgcgtcccg gatcacgtag
     2341 ccgccggcca gccccgcgaa cacgttcagc cgggtcgcgc ccgtcgcatg gtcgtggtac
     2401 cacagcagcg tcgcgcgctg gttgttcgga tagaacgccg actgagtgtc gccgaacagc
     2461 acgcccccct ccgaagaggc ggccggattg ccatcgttta ggccagagtc aaacgccccg
     2521 tgcaggtgcc cgatgatgcg cgtgcggttg tcgccgtccg tcagattcct gtcgaccggg
     2581 atccgcggga aggggtaggt ggccggtagc ccgtttgaat aggtgagcgt ggcggcggac
     2641 ccgctatgga ccacgatggt cgggccgaga tagccgctgt acgccgagga gccgtcgtcg
     2701 taggaccacg ggtgggtcgc tccggcgagc tgcggatgca actgtctcgt gatctccttc
     2761 gcggtgaagg catggatatc gccagcgccg agcgcgaccg cgggcggaac cgcgaacggc
     2821 atcatgtacg acgtcaggtt cgccgtcggc gcaaccgcgg tagcggcacc ggcctgcaac
     2881 agcacgttac cgaccatgat cccgcccgcg cctgccgcgc ccctcttgat gaactgccgt
     2941 ctggtgagca tgtcgccctc tcctttctca gacgcgcaga atcgacgcca gcgcccagca
     3001 ggtcactacc cctggacctt tagtccagtt gttacggggt ggacccctct cacggagccc
     3061 gagttcacgc atccggcttt ttagaccttt gatgggcaat ggtcggcaat tgcaggcccg
     3121 ccgacatcgg tgcatgctcg cgaacacgac acgtactaat ccacatcatg cttgtcgaat
     3181 ccttgccgtc gccctaggaa gcaaggtgct ggcggcatct gccggcagcc gcggagcgct
     3241 gcctccattc gatagagcac agccgcgtca tgtgccggcc caggcagtga gcgtcgcacg
     3301 ctcgtgcgcc gtcgcgaccc ggacgtaccc cgagcggcgt gggggtcact gtgatttggc
     3361 gtcgagcgtg acctcagctt cctgcggcgg ctgagaacat tctcacccgg tcatgccaag
     3421 gcttcgaaca tcgcccgtta ccgtcgagaa cggccgcatc ggcccttacg catccgggcg
     3481 ctgcggccac ctaccaccca cgtcgcagat caagcgccag ctacccggca gcgcgatcca
     3541 gacgtctact ctgcgctgat atccggccat cgtcgaagga cggtccaaaa gactcgcgtc
     3601 agccccgcag cgcaaaccag ctcccgcata ggcaggcggc tgactgccca gttgggcccg
     3661 ctcgctgtgt tccgccgatc ggtcgcgccc cgactccggt cctccgccca cccgtagcgg
     3721 cgaaaccgtg ctcggctccg caaagcattg atcacctagt tcgcccggat ctcaggaatc
     3781 tggcaccctc tatcacgagc ggagctggcg ctgcgggtca agcagccgag cggtacgcag
     3841 cacgctgccc gaacacacct actgcgaccg ccgcgcaggg tcggacagaa tgctgctctg
     3901 atcgaacagc agcgacgacg ctcgactgct gcttgcgttt ggaggcaagg ctctcgcacc
     3961 actgtcacgc gaggtccggt cccaactcgc gtcgacttct ccagcgccgg gcggatccag
     4021 cagcctccca tacgcggcga gaacgcggga cctcgtcgga gcgcaagaga ttttcgaagc
     4081 gctcggcgtg gctgctttgc ctacgtacgt gaccgcctcg ctcagcggcg tcgggctttg
     4141 gggcttgcga cggtaactac cagctgatgg ttgacgcaag cgcctcggtg ttgatggcga
     4201 atttccggac tactatcgca atccgcggga ggcgcacggc ggccggcggc tcaacgtgcc
     4261 tgctgcgaac gcgctgccga cctgtttctg gtgcgagtcc agcaggccta ctgcgtatgg
     4321 ggcgctccgg gcatctaccc gttctcgcgg ttgcgcgtcc ctttgctttc ttcaggtgtt
     4381 tgtgtgggct acttacgcag gccgatgtcg gtgaagtacg tcatcccgtc gtcgagcttg
     4441 acgccgatct tccagtagta gccgccttgg gtcgacgagg tgttccagtt gtagatgtac
     4501 tgctgggcgg tgctgtccca gcggaagccg cttgcggagt cgccggtcgt ggtgaacgag
     4561 tcttcgttga ccgcgctggt ggtcaggttg ccctgtgcgg gggtttgcca gatcggggcg
     4621 ctgttggctt gaacgatctg cccggctgcg ttcttgagct ggaacttcac cggcacggtt
     4681 tgccccgcct tgaagatgct cagggtcgtg gacgcggtgt gcgcggtgtc gttgatgggc
     4741 tgcaggaaga acgcgacgtt tgcggggacg ttgtagatca ccttgtagga gaccgactgc
     4801 gtgaccaagt tgccggcttt gtcggtggcc gtggcggtgt aggtgaaggt cccgaccccg
     4861 tttgggagcc cgccgcttag gctgcccttg cagctggcga ccccggagaa cgagtccgtg
     4921 gcattgcagg tcggcgtggg caccgatccg agcgtgtaga tgccgccgtc cttgactccg
     4981 ccgatcgtca gcgtggggtt ctccatgtcg atgttgatcc cgctcgcggt cttgctggcg
     5041 gtgttgtcgg ccttgtcggc taccgacctg gtcacggact gattggcgcc gttttggctg
     5101 agggtcacgg gatccgggca ggtcgcgacc cccgagccgg cggcgccgtt cgccgcggcc
     5161 gggtcggcgc agctgaaggt gacgagcacc gcgtggacgt accagccgtt gtttccgtct
     5221 gggctcccgg gggtgaccgc ggcggagatc gtcggcgcgg ttttgtcgag gtggaagtgg
     5281 aaggagtcgg tgccgtggtt tccggcgttg tcgtcggccg agccggtgat cagttgatcg
     5341 gcggtctcgg tcgagtaggt ctggtccggg gtcagggttc ccgtgttgac gccggagccg
     5401 ccggaatcgt ccgtggcgtt gaagtgcacc agggtgttgg cgttgctcca gtcggccgcg
     5461 ttggcgagcg gggcgagcgt gtgcgagaca gccggtggcg taccgtcgta gttgatggag
     5521 tcggcatgct tgtcctcggc gttgccggcc ttgtcgaccg cgtagtagta ggcggccgct
     5581 gttccgctcg ccgacagggt tatcggcacg ttcaccgagc tcccggtggc cgtcatccag
     5641 gtggctccgc cgtccttgct gtagtggatc tccttgacgc cggacacacc ggtcacgccg
     5701 gtcggcgttg ggtcggaggc gttgatcgtc atcgacaccg gggaaccggc gttgcaatag
     5761 ttgccgacta gggtgcagtt caccgtgtcg gtagacaccg gcggggtgtt gtcgatgttg
     5821 atgccgccga cggttttgcc ggcggtcgag ttgcccgcgt agtccttggc cgcatcgctg
     5881 gtgacgctga ggttggcgcc ctcgtcggtg atggcgatgt ccgaggggca gctcgcgacg
     5941 ccggagccgg ggctgccgtc ggccagatta ggatcggcgc agccgaacgc gaccttgacc
     6001 agcccgctgt accagccgtc ctggttgggc tgcgtggtcg gggcgcccga gatcaccggg
     6061 ccgttgcggt cgatattgac gtcggtgacc gaggcaaagc ttgagttgcc ggccttgtcg
     6121 gacacgctca ccggcccggc gcccaggtgc cggccctcgc cgctgatcgt gccggcggcc
     6181 gggcagggcc cgtcgatccc ggacagaccg tccgcgcagg cccagttgac ggcaacgtcg
     6241 ccggtgtacc acttggcccc ggttgagtcg atcccgttcg gatcgggcag ctgcccggcg
     6301 atgtccggcg gggtcaagtc gatgttgatg cccgtgacca tcgtctccgc cgtgttgccg
     6361 gcgttgtcgg ccgcgttgcc ggtcacgaac tggcccgcgc cctcggtgga gaccgtggtc
     6421 gggtccgagc aaccggcgac gccggactcc tggtcggcgc agctgaagct gaccgtgacc
     6481 ggctggttgt tccagccgtt ctgattggcc gccggggtcc gcgcgccggt gatcgtcggc
     6541 cggacgttgt cgatctttag cgtgacggta tggccggcgg cggtctggtc ttcggtgttg
     6601 ccggcgtcgt cgacgctcca aaaggagatc gtgtgcacgc ccttgagcgc gaagtcgaac
     6661 gggccgctgt aggcctgcgc ggcgccgctg tcgaccgcgt agtaagtctt gtccacgccg
     6721 gacagcccgt cgtggccggt cagagtgacc ttcgcggcat tggcgtacca gccgctgtcg
     6781 agtggatcag tcacgctcac cgtggtcgtc ggcggcgtgc ggtcgatctt gatgtggtcc
     6841 accgccgtcg ccgtggtgtt gccggccttg tcggacacca ccgcgctggc ggaaaggttg
     6901 tccgactcgc cgctgatcac gctatcggcc gggcagccgc cgtcgactcc cgagagcccg
     6961 tcggagcacg tccagtggac ggtcacatcg tcgcggtacc agccgttcgc gttcggtgcc
     7021 gtcgtggcgg cgccggcaag ggtgggcgcg ctcttatcga cgttgatgtg gctgacgccg
     7081 tccgaggccg agttgccggc cgcgtcggtc gcagttccgc tcgtggactg gtcggcgccc
     7141 tcgcccagcg tcttcgggac cgggcacgtg tcgatgcccg acagcgcgtc ggcgcactcg
     7201 aagctcacca cgacatcgtc catgtaccag ccggcggcat tcgccggccg gtcaacgctg
     7261 gccttgatcg tcggcgcggt cttgtcgatc gagaccttcg ccggatcggt ggtcgcgttg
     7321 ccggcgttgt cgatggcggt cccggtcacg gcctggtcct tgccctcggt gctcaccgtc
     7381 tgcggacccg tgcacgaagc gatgccgctg ccggcgatgc cgctgctgcc gggatcggcg
     7441 caggcgaagg tcacggtcac gtcactctgc agccagccat tggcgttggg cagcgggtcg
     7501 aacacatcgg tgatggtggg cggcgtcttg tcgatcttga cgttgacggt cttggccgcc
     7561 tcggtgttgc ccgccttgtc gacactccag aactgcaggc tgtgcgtgcc ttcggcagag
     7621 atctggaccg aggcgccgct ctgctgcggg ccgccgtcga gctcgtaaaa cgtgtcggcc
     7681 acgcccgaca gcgcgtccgc ggcgtcgagc gacaccgtga cgtccgtgtt attccaattc
     7741 gttggtgcgg tcgcggtcgt gagaggcgcc gtcttgtcga tcttcaccgc cggcgagctc
     7801 ggggcgttcg tcgtgttgcc ggccttgtcg aagacagacg cctgcgcggt gaggccggtg
     7861 ccctcgctcg agatcgtgct gtccgtcggc gatgagccgg cgaggccgga gagcgcatcg
     7921 ctcgcgctcc agtggatggt gacgttgccg ttgtaccagc ccgcatcgtt tggactcgtg
     7981 gtgggagcgc ccgtcagcgt cggcgccacc ttgtcgatgt tgatcccact gacgctcgcc
     8041 gatgccgagt tgtcagcgtt atccacggcc gttgcggtaa cggactgatt ggcagcgctg
     8101 tcggtcagcg tggtcggacc ggagcagctc ttgatccccg ccaagctgtc cgagcagcta
     8161 aagccgacgg tgacatcgct gttgttccag ccgttcgcgt tggctgccgg ggaccggttg
     8221 ccggtgatcg tcggcgcggt cttgtcaagc ttgatcgtca ccgaaccaga tccggagttg
     8281 cccaggcggt cagtcgcagt cgacgtcttg gtcacaccgg ccgtgttgga cgtctgcgag
     8341 tccgtcgccg gggtcggtcc gctggccacg cccgacccgg cgtcggccgc cgaccacgtc
     8401 accgaaacgt tcgaatcgtt ccaacccgcc ccgttaggcg tgggcgagag cgccgcagtg
     8461 acgaccggac cggtgttgtc gaggatgaag gacgccgacc ggggactctg cgactggcct
     8521 gtgcagttgt tagcgttgaa gttgggcgat gcggtggcgg tgactgtctt aacgccgtcg
     8581 cccgtgctgg ccgtgaagtt gaaggtccag ctggacttcg cggagctcga tgtctggtgc
     8641 ccggtgaacg cgccagtgac gtcgacgcac ttcgtgtcgc tggaggtgtt gacagtgagc
     8701 gtcaacgcac cgccctgctt ggcgtagaga acgctcccca cgctcaccgt accgctcccg
     8761 ccggtgaagt tcgccgagct gaccgtcgag gcgattcccg cggcggacat cgccagaacc
     8821 gacgccagcg tgaacgaaac gagcgccgca aggatcgcgg cccgacgcca caacccgctc
     8881 gccgcccgcg cgccagtctt gctgcgagat aacatcctcg cccccttttc agctgtgcca
     8941 aagcgcgacc acaccgtcgg gcctgttgtg caggtccatc cgcggcgtat tacgcccaca
     9001 ccttctatgg agataaacgt aagctgctag gcaaagcttg tatcgccctg gcgcggccgc
     9061 gccctctgca catcgaggcg gcttgctcag gccctcgcta tcaaccgaca gaaggcgaag
     9121 gccagcagag cccgcactac cggcgaacgg acgcacgctt tc
//
LOCUS       DASSGQ010000042         9146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1065875,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000042 DASSGQ010000000
VERSION     DASSGQ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9146
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065875"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VFI55_01930"
     CDS             <1..275
                     /locus_tag="VFI55_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET7072827.1"
                     /translation="SGATVSGQGNDMVLDTLSFYKPTQPGAYPIVLATYEIVCSKYPD
                     SQVGTAVKAFLQSTIGAGQDGLGDNGYIPIPDSFKSRLSASVNGIA"
     gene            287..1279
                     /gene="pstC"
                     /locus_tag="VFI55_01935"
     CDS             287..1279
                     /gene="pstC"
                     /locus_tag="VFI55_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET7072828.1"
                     /translation="MTRGVIAGPSTGTQPALKPTDSRKVRRGDRLFKLAATAAGSTIV
                     IAIVLIAVFLLVRAVPSLRADRVNFFTSAEFDTTKAEHLAFGIRDLFMVTALSSMGAL
                     VLAVPIAVGIAVFLTQYSPKRFARPFGAMVDLLAAVPSIIFGLWGIFVLAPKLEPFAE
                     LLNRRLGWLFLFKQGNVSLAGGGTIFTAGIVLSVMILPIITSVSREVFRQTPHLQMEA
                     AQALGATKWEVVRMTVLPFGRSGVVAASMLGLGRALGETVAVLIILRSAARPGNWSLF
                     DGGYTFASKIASAAAEFSEPLPTGAYISAGFALFVLTFIVNAAARAIAGGRVNG"
     gene            1314..2216
                     /gene="pstA"
                     /locus_tag="VFI55_01940"
     CDS             1314..2216
                     /gene="pstA"
                     /locus_tag="VFI55_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET7072829.1"
                     /translation="MTIDALDLPVKAEVFTPLSMRRRITNNAAATFFIASFLVALVPL
                     IWVLSVVVARGWYAVTRSGWWTHSLRGVLPEEFAGGVYHALYGTVVQAGVAAVLSVPL
                     GLMTAVLLVEYGTGRLVRVTTFMVDVLAGVPSIVAALFVFSLWIVTLGFQQSAFSVSL
                     ALVLLMLPVVVRSTEEMLRLVPNDLREASYALGVPKWKTIVRIVFPIAMPGIVSGVLL
                     SVARVIGETAPVLVLVGYSRSINLDIFHGNMASLPLLIYTELTNPEHAGFLRIWGAAL
                     TLIIIVAVINLLAAAFRLLATRRR"
     gene            complement(2233..3126)
                     /locus_tag="VFI55_01945"
     CDS             complement(2233..3126)
                     /locus_tag="VFI55_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET7072830.1"
                     /translation="MRSIWKGSIAFGLVNVPVKVYSATEDHDIKFRQVHAKDNGRIRY
                     QRVCEADGEVVEYRDIARAYESDDGQMVVITDDDIATLPEERSREIEVLEFVPASEVD
                     PLLFDRSYFLEPDSKSSKSYVLLAKTLAETDRMAIVHFTLRNKTRLAALRVKDFGKRD
                     VMVVHTLLWPDEIRDPDFPVLDQKVDIKPAELKMAGQVVDSMAEDFNPDRYHDTYQEQ
                     LQELIDAKLEGGEAFTTEERPKELDETEDVSDLLAKLEASVKARTGDGKAPAKKTPAK
                     KAQAKKAPAKQAAARKAASKS"
     gene            complement(3259..4179)
                     /locus_tag="VFI55_01950"
     CDS             complement(3259..4179)
                     /locus_tag="VFI55_01950"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HET7072831.1"
                     /translation="MARGLVIGEALIDIVEHQGATERVGGSPLNVAVGLARLGREIDF
                     LTHIGTDEPGRRIASYLKSSGAQLVPGSTSARRTPSATATIADDGSASYAFDLEWQLS
                     GTPEVAPPLFVHTGSIAAVQEPGCLAVAALLEAYRLSATVTFDPNVRPSLIVDPDLAR
                     ERIAHVVERSDIVKASDDDLRWVDPEHEPEWTARGWLASGPALVVVTMGDRGAVAFCS
                     RGEVRVAARPVQVIDTVGAGDAFMVGLLDALWGLDVLGGGRRAALGEIGADALTAALE
                     AGSLSSALTLGRAGADLPDRATLQAQSGFA"
     gene            complement(4192..5613)
                     /locus_tag="VFI55_01955"
     CDS             complement(4192..5613)
                     /locus_tag="VFI55_01955"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HET7072832.1"
                     /translation="MVGLNSATLSNLPIATPSYDRDGVQVGIAHIGVGGFHRAHQAMY
                     IDTLLERGAAREWGICGIGVMPADRRMRDVLRAQDGLYTLVLEHPDGARDARVIGSIV
                     DYRYAPDDPPATVELLAAPATRIVSMTITEGGYQIRDPGSVFGLIVGALAARRDRGIP
                     APTIVSCDNIEHNGEVARRAVVEHADLLRPGLADWIAETTRFPCSMVDRITPVTTPDI
                     AAGVERDFGITDAWPVVAEAFASWVLEDDFADGRPPLEDAGVLLVDDVSPYESMKLRL
                     LNAGHQCLCYFAWLCGYRMVHDAARDPLFAEFLLAYFDSEAVPTLRPVPGIDLRAYTR
                     GLIERFANPGVGDTVARLCAYSSDRIPKWLLPVIRDNLLTGGPIRLASAAVASWARYA
                     EAVDERGEPIDVVDNLAASLIPTARSQRLHPAAFIENAALFGDLAAAPRFVDAYSRAL
                     DSLHTHGARATLDLLLHGFNSGV"
     gene            5661..7973
                     /locus_tag="VFI55_01960"
     CDS             5661..7973
                     /locus_tag="VFI55_01960"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET7072833.1"
                     /translation="MGAAASGAQARVQLTNADKVLYPATGDGRTAVTKGDVFDYYTRI
                     ADAMLPHIAGRPATRKRWPNGVEQAAFFEKQLASSAPDWLPRVSVTHRSGTTTYPIID
                     SPEALAWIAQQAALEVHVPQWRFVAEWTRSKSEELKPGPATRLVFDLDPGEGVTMAQL
                     AEVARAVRDLIADIGLTTFPLTSGSKGLHLYTPLDEPVSSKGATVLAKRVAQQLEKTM
                     PKLVTSTMTKSVRAGKVFLDWSQNNGSKTTIAPYSLRGREHPTVAAPRSWEELDDPAL
                     CQLRYDEVLDRLAREGDLLAPLDTRSPVPDRLSKYRNMRDASKTPEPVPAGKPGKPVT
                     GQGNTFVIQEHHARRLHYDFRLERDGVLVSWAVPKNLPETTSVNHLAVHTEDHPLEYG
                     TFEGSIPKGEYGAGKVIVWDSGTYEAEKFRDDGEKGEVIVNLHGSRISGRYALIQTNG
                     NQWLAHRMKDQTVFVFDEIAPMLASEGSVAALKAGQWAFEGKWDGYRLLVEVDRGALR
                     VRSRSGRDVTAEYSQLDSLATDLADHHVVLDGEAVVLDKSGVPRFNEMQNRGRGTPVE
                     YWAFDLLYLDGRSLLRAKYQDRRKLLETLSSATNLIVPELLPGDGTEALGYSREHGWE
                     GVVAKKRDSSYQPGRRSASWIKDKNWNTQEVVIGGWKAGEGGRTSGIGSLLMGIPGAG
                     GLHFAGRVGTGFTERDLANLKQTLAPLRTEQSPFDAPLPARDRKGVTFVEPLLVGEVR
                     YSEWTPDERLRQSSWRGLRPDKKPSEVVRE"
     gene            7970..>9146
                     /locus_tag="VFI55_01965"
     CDS             7970..>9146
                     /locus_tag="VFI55_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET7072834.1"
                     /translation="MKWATYQGGNDGTAERTGVLSGDVIHALPSGVTLLELVGRGADG
                     LREAGVQALKSPAAVARLGEVTLKAPIPRPPSIRDFLCFLDHMRNCQAAMGAGRELAD
                     TWYRIPAFYFACPATVLGPYDDAPMAPGSAWQDFELEIAAVIGTRGKDLTVEQAEQAI
                     IGYTIFNDWSARDLQQLEGQLAIGQAKGKDSGVTLGPFLVTPDELEPYRRDGKLDLQV
                     TALVNDTVIGSGSTAQMDWSFAEVISYASRGVTLHPGDVIGSGTVPTCTLVEHLNPTA
                     LESFPGWLHDGDVVTLRVEGLGETRQVVRASRAPHPLAARPNPSAKTAAARVNRAPAR
                     VPYTRGLHEVAERVWAWTLPDGGYGWSNAGLVAGDGASLLVDTLFDLALTREMLAAMK
                     "
BASE COUNT         1522 a         2969 c         3051 g         1604 t
ORIGIN      
        1 tttccggtgc caccgtgagc gggcagggca acgacatggt gctggacacg ctgtcgttct
       61 acaagccgac gcagcccggc gcctacccga tcgtgctcgc gacctacgag atcgtctgct
      121 cgaagtatcc ggattcccag gtcggcacgg ccgttaaggc attcctgcag agcaccatcg
      181 gtgccggaca ggatggcttg ggggacaacg gatacattcc cattcctgac tcgttcaagt
      241 cgaggttgtc ggcgtcggtc aacggtatcg cgtgatccgg agtggcatga cccgcggagt
      301 gatcgccggc ccgtcgacgg ggacccagcc cgcgctgaag ccgaccgatt cgcgcaaagt
      361 gcggcggggc gaccggctgt tcaagctggc cgccaccgct gccggttcga cgatcgtgat
      421 cgccatcgtg ctgatcgcgg tattcctgtt ggttcgtgcc gtcccgtcgc tgcgcgcgga
      481 ccgcgtgaac ttcttcacca gcgccgaatt cgataccacc aaggcggagc acctggcgtt
      541 cggtatccgc gacttgttca tggtcacggc gctgagttcg atgggcgcgc tggtgctggc
      601 cgtgccgatc gcggtcggga tcgcggtttt cctcactcaa tactcaccga aacggttcgc
      661 gcgtccgttc ggagcaatgg tcgatctgct ggccgcggtg ccgtcgatca tcttcgggct
      721 gtgggggatc ttcgtgctgg cgcccaaact cgagccgttc gcggagttgc tcaaccgcag
      781 gctgggctgg ttgttcttgt tcaagcaggg caacgtttcc ctggccggag gcggcaccat
      841 tttcaccgcc ggcatcgtgc tttcggtgat gatcctgccg atcatcacat cggtgtcgcg
      901 agaagtgttc cggcagactc cgcacctgca gatggaggcc gcgcaggcac tgggtgccac
      961 gaagtgggag gtggtgcgga tgaccgtgct gccgttcggc cgtagcggtg tcgtcgccgc
     1021 gtccatgctg gggttgggcc gcgctctggg agaaaccgtg gcggtgctga tcatcctgcg
     1081 ctcggccgcc cgtccaggga attggtcgct gttcgacggc ggctatacgt tcgcttccaa
     1141 gatcgcttcc gcggcggccg aattcagcga accgctgccc accggagcct acatctcggc
     1201 gggatttgcg ctcttcgtcc tgacgttcat cgtcaacgcg gccgcccgtg cgatcgccgg
     1261 cggaagggtc aacggatgac ccgcgcgcga agcgttggga aggagcggcg cgcatgacca
     1321 tcgatgcgct cgacctgccc gtcaaagcgg aggtgttcac ccctctgagc atgcggcggc
     1381 ggatcacaaa caacgccgcg gcaacgtttt tcatcgcctc gttcctggtg gctctggtgc
     1441 cgctgatctg ggtgctatcg gtagtggttg cgcgcggctg gtatgccgtc acccggtcgg
     1501 gctggtggac ccactcactg cgcggcgtgc tgcccgagga gtttgccggc ggtgtgtacc
     1561 acgcgctgta cgggacggtg gtgcaggccg gtgtcgccgc cgtcttgtcg gtgccgctgg
     1621 gattgatgac cgcggtgctt ttggtggaat acggtactgg ccggctggtg cgggtgacga
     1681 ccttcatggt cgacgtgctg gccggggtgc cctcgatcgt cgcggccctg ttcgtcttca
     1741 gcctctggat cgtgaccctg ggatttcagc agagtgcctt ctccgtgtcg ttggcgttgg
     1801 tcttgctgat gttgccggtg gtggtccggt ccaccgagga gatgctcagg ttggtgccta
     1861 acgacctgcg agaagccagc tacgcgttgg gtgttcccaa atggaagacc atcgtgcgga
     1921 ttgtcttccc gatcgcgatg ccgggcatcg tctccggtgt gttgctgtcc gtcgcacgcg
     1981 ttatcggcga aaccgctccg gtgctggtgc tggtcggata cagccggtcc atcaacctcg
     2041 acatctttca cggcaacatg gcctcgctgc cgctgttgat ttacaccgaa ctcaccaatc
     2101 cagagcacgc cggttttttg cgcatctggg gcgcggcgct gacgctgatc atcatcgtgg
     2161 ccgtgatcaa cctgctcgca gcggcgttcc gcctcttggc gactcgccgg cgctgatccg
     2221 gcccggcccg ggtcaggatt tcgacgcagc ttttctagcc gccgcctgct tggcgggcgc
     2281 ctttttggcc tgtgccttct tcgccggtgt ctttttggcg ggtgccttgc cgtcgccggt
     2341 gcgcgccttc acgctggcct ccagcttggc cagcaggtcg gagacatctt ccgtctcgtc
     2401 cagctccttg ggccgctcct cggtggtaaa tgcttcccca ccttcgagtt tcgcgtcgat
     2461 gagttcctgc aattgctcct ggtaggtgtc gtggtaacgg tcggggttga agtcttcggc
     2521 catcgaatcc accacctgcc cggccatctt gagctcggcg ggcttgatgt cgaccttctg
     2581 atcgagcacg ggaaagtcgg gatcgcggat ctcgtcgggc cacaacaatg tgtgcaccac
     2641 catcacgtcg cgcttaccga agtccttgac gcgcaacgcc gccaaccggg tcttgttgcg
     2701 caacgtgaaa tgcacaatcg ccatccgatc cgtctcggcg agcgtcttgg ccagcagcac
     2761 ataggatttg gatgacttcg agtcaggctc caggaagtaa ctgcggtcga acagcaacgg
     2821 gtcgacctca ctggccggga cgaattccag cacctctatc tcgcgactgc gctcttcggg
     2881 caaggtggcg atgtcgtcgt cggtgatcac caccatttgg ccgtcgtcgg attcgtaggc
     2941 ccgggcgatg tcacggtatt cgacgacctc gccatctgcc tcgcacacgc gttggtatcg
     3001 gatgcggccg ttgtccttgg cgtgcacctg acggaacttg atgtcatggt cttcggtggc
     3061 gctatacacc ttgaccggaa cgttcaccag cccgaacgcg atcgagccct tccagatgga
     3121 gcgcatgtaa gtcagtatgc ccatacggcc cctagcaaaa cagcgcgacg ggcagttgag
     3181 gtttgctgaa agcctgcgag cgtaacgctg ctgcgatttc aagccggttc tttcgcgctg
     3241 gtgttacgct cgcggctttt aagcaaagcc ggattgagcc tgcagcgtgg cccggtcggg
     3301 taggtcggcg ccggcgcggc cgagggtcag cgcggacgac aggctgcccg cctcgagcgc
     3361 ggcagtcagc gcgtcggccc cgatctcgcc cagggccgct cgccgtccgc cgcccaacac
     3421 gtccagtccc cagagcgcgt cgagcaggcc caccatgaac gcgtcgccgg cgccgacggt
     3481 gtcgatcacc tgaaccggcc tggcggccac ccggacctcg ccgcgcgaac agaacgccac
     3541 cgcgccgcga tcgcccatcg tcacgacgac gagcgctggc cccgacgcca accagcctcg
     3601 ggccgtccac tccggttcgt gctcgggatc gacccaacgc aggtcgtcgt cacttgcctt
     3661 gacaatgtcg ctgcgctcca cgacgtgggc gatccgttcg cgggccagat ccgggtcgac
     3721 gatcagcgag ggacgcacgt tcgggtcgaa ggtcacggtg gccgacaaac gataggcctc
     3781 gagcagcgct gccaccgcca ggcaccccgg ctcctgcacg gcggcgatgg atccggtgtg
     3841 cacgaacagc ggtggcgcga cttccggcgt gccggagagc tgccattcca ggtcgaacgc
     3901 gtaggacgcc gatccgtcgt cggcgatcgt cgccgtcgcg ctcggcgttc ggcgcgccga
     3961 cgtgcttccc ggaacgagct gcgcgccaga ggatttgagg taactagcga tgcggcggcc
     4021 gggttcatcc gtgccgatgt gggtcaagaa atcgatctcg cgccccagcc tggcaagccc
     4081 gacggcgacg ttgagcgggc tgccgccgac acgttcggta gcgccctgat gctcgacgat
     4141 atcgatcaac gcttcgccga tcaccaggcc gcgcgccata gattcacccc ttcacactcc
     4201 actgttgaag ccgtgtagca acaggtccag cgtcgcccgt gccccgtgag tatgcaatga
     4261 atccagcgcc cggctgtagg cgtcgacgaa tcggggcgcc gctgccaggt ccccgaacag
     4321 cgccgcgttt tcgatgaagg cggccggatg cagccgctgc gaccgtgcgg tcgggatcag
     4381 cgaggccgcc aggttgtcga cgacgtcgat cggttcgccg cgctcgtcga ccgcttcggc
     4441 atagcgggcc cagcttgcca ccgccgccga cgccagccgg atcggcccgc cggtcagcag
     4501 gttgtcgcgg atcaccggaa gcagccattt cggaatgcga tccgaggaat aggcgcacaa
     4561 ccgcgccacc gtatcgccca ctcccggatt ggcgaaccgc tcgatcagcc cacgggtgta
     4621 ggcacgcaga tcgatgccgg ggacgggccg cagcgtcggc accgcttcgg aatcgaaata
     4681 cgccagcagg aattcggcga acagcgggtc gcgcgccgcg tcgtgcacca tgcggtaccc
     4741 gcacagccac gcgaagtagc acaggcactg gtgaccggcg ttgagcagcc gcagcttcat
     4801 cgactcgtac gggctcacgt cgtcgacgag caggacgccc gcgtcttcga gtgggggcct
     4861 gccgtcggcg aagtcgtcct cgagcaccca cgaagcgaac gcctccgcca ccaccggcca
     4921 cgcgtcggtg ataccgaagt cgcgctccac tccggccgcg atgtccgggg tggtgacggg
     4981 cgtgatgcgg tccaccatcg aacacggaaa ccgggtggtt tcggcgatcc agtcggccag
     5041 gcccgggcgc aggaggtcgg catgttcgac gacggcgcgc cgcgcgacct cgccattgtg
     5101 ctcgatgttg tcgcacgaga cgatcgtcgg cgcggggatg ccgcggtcgc ggcgcgcggc
     5161 caacgccccg acgatcaagc cgaacacgga tcccgggtct ctgatctggt agccgccctc
     5221 ggtaatggtc atcgagacga tgcgggtcgc cggtgcggcc agtagctcga ccgtggccgg
     5281 cgggtcgtcg ggcgcgtaac ggtagtcgac gatcgagccg atcacacgcg cgtcccgggc
     5341 gccgtcgggg tgttccaaga ccagggtgta caggccgtcc tgggcgcgca agacatcccg
     5401 cattcggcgg tcggccggca ttaccccgat tccgcaaatg ccccattccc gcgcagcacc
     5461 gcgctcgagc agcgtgtcga tgtacatcgc ctgatgtgcc cggtgaaacc cgccgacccc
     5521 gatgtgcgcg atgccgacct gcacgccgtc acggtcgtag ctcggtgtcg cgatcggcag
     5581 gttgctcagg gtggcgctgt tgagcccaac cacggtgtcg acggtaccgg cgcgcgccga
     5641 gttttcagta cgttgacacc atgggtgcgg cggcttccgg ggcgcaggcg cgggtccaac
     5701 tgaccaacgc cgacaaggtg ctgtaccccg cgacagggga cggccgaacg gccgtcacaa
     5761 aaggcgacgt cttcgactat tacacccgca tcgccgacgc gatgctgccg cacatcgccg
     5821 gacgcccggc cactcgcaag cgctggccga acggcgtcga gcaggcagcg ttcttcgaaa
     5881 aacagctggc gtcgtcggcg cctgactggc tgccccgcgt cagcgtgacc caccggtccg
     5941 gcacaacgac ctatccgatc atcgacagtc ccgaggcgct ggcctggatc gcgcagcagg
     6001 cggcgctgga ggtacacgtg ccgcagtggc gcttcgtcgc cgagtggacc cgaagtaagt
     6061 ccgaagaact caagccagga ccggcgacgc gattggtgtt cgacctggat ccgggcgagg
     6121 gcgtgacgat ggcccaactt gctgaggtgg cgcgcgccgt ccgggatctg atcgccgata
     6181 tcggactgac cacttttccg ttgaccagcg gcagtaaggg gttgcacctt tacacaccgc
     6241 tggacgagcc cgtcagcagc aagggcgcca ccgtattggc caaacgtgtt gcgcagcaac
     6301 tggaaaagac gatgcccaag ttggttacgt cgacgatgac caaaagcgta cgggccggca
     6361 aggtcttcct ggactggagc cagaacaacg gctcgaagac caccatcgcg ccgtattcgc
     6421 tgcgtggacg cgaacatccg accgtcgcgg caccacgcag ttgggaggaa ctcgacgatc
     6481 ccgcactgtg ccaattgcgc tacgacgagg tgctcgaccg gctggcgcgc gagggcgatc
     6541 tgctggcgcc gctggacact cgatcgcccg tcccggacag gctgagcaag taccgcaaca
     6601 tgcgcgacgc gtcgaagacg cccgagccgg tacccgcggg gaagccagga aaacccgtta
     6661 ccggccaagg caatacgttc gtcattcagg agcaccacgc ccgtcggctg cactacgact
     6721 tccggctgga gcgcgacggt gtgctggtgt catgggcggt gccgaaaaac ctacccgaga
     6781 ccacgtcggt gaaccacctc gccgtgcaca ccgaagatca tccgctggaa tacgggacct
     6841 tcgagggcag catccccaaa ggcgaatacg gcgccggcaa ggtgatcgtc tgggactccg
     6901 gcacctacga agccgagaag ttccgcgacg acggtgaaaa aggggaggtc atcgtcaatc
     6961 tgcacggcag ccgaatctcc gggcgttatg cgctgattca aaccaacggc aaccagtggc
     7021 tggcgcaccg gatgaaagac cagacggtct tcgtattcga cgagatcgca cccatgctcg
     7081 caagcgaagg gtcggttgcg gcgctgaagg ccggccaatg ggcattcgaa ggcaaatggg
     7141 acggctaccg gctactggtc gaggtggatc gcggcgccct gcgggtgcgg tcccgcagcg
     7201 gacgcgatgt gaccgcggag tattcccaat tggattcact ggcaaccgat ctcgccgatc
     7261 atcatgtggt gctcgacggc gaggccgtcg tcctcgacaa gtccggtgtg ccgcgcttca
     7321 acgagatgca gaaccgcggt cgcggcaccc ctgtcgagta ctgggcattc gacctgctct
     7381 acctcgacgg ccgctcgctg ctgcgcgcga agtatcaaga ccggcgcaag ctactggaaa
     7441 ccctttccag cgcaaccaat ctcatcgttc cggagctgct gccgggggac ggcaccgagg
     7501 cgctcgggta ttcccgcgag catggctggg agggcgttgt tgccaagaaa cgcgattcga
     7561 gttatcagcc gggtcggcgc tccgcgtcgt ggatcaagga caagaactgg aacacgcagg
     7621 aagtcgtcat cgggggctgg aaggcgggtg agggcgggcg taccagcggc atcggctcgc
     7681 tgctgatggg aattcccggc gccggcggac tgcatttcgc ggggcgggtc ggcaccggct
     7741 tcaccgaacg tgacctggcc aacctgaagc agacgctggc gccgctgcgc accgagcaat
     7801 ctcccttcga cgcaccgctt cccgcgcgcg acaggaaggg tgtgacattc gtcgagccgc
     7861 tgctggtggg cgaggtgcgc tacagcgagt ggaccccgga tgagcgcttg cggcaatcga
     7921 gttggcgcgg gctgcggccg gacaagaagc caagtgaggt ggtacgcgaa tgaaatgggc
     7981 tacgtatcaa gggggcaacg atggcactgc cgagcgcacc ggggtgctct ccggcgacgt
     8041 gatccacgcg ctgccgtcgg gggtgacgct gctcgagctg gtcggacgcg gcgcggacgg
     8101 gctgcgcgag gccggcgtgc aggcgctgaa atcgcctgcg gcggtggcgc gtctgggcga
     8161 ggtgacgctg aaggcgccga ttccgcggcc gccgtccatc cgtgatttcc tgtgcttcct
     8221 ggaccacatg cgcaactgcc aggccgcgat gggcgccgga cgggaactcg ccgacacctg
     8281 gtatcgcatc ccggcgttct acttcgcctg cccggcgacc gtccttggac cctatgacga
     8341 tgcgccgatg gcgcccggaa gcgcttggca ggatttcgaa ttggagatcg ccgcggtcat
     8401 cggaacccgc ggcaaagact tgacggttga gcaggccgaa caagccatca tcggctacac
     8461 catcttcaac gactggtccg cgcgcgactt acagcagttg gagggccaac tggcgatcgg
     8521 gcaggccaag ggcaaggata gcggcgtcac gctgggcccg tttctggtca ccccggacga
     8581 gctggagccg taccgccgcg acggcaagct cgatctgcag gtgaccgccc tggtcaacga
     8641 caccgtgatc ggttcggggt cgaccgcgca gatggattgg agcttcgccg aagtcatctc
     8701 ctatgcctct cgcggggtaa cgctgcaccc cggcgacgtc atcggctccg gcaccgtacc
     8761 cacgtgcacg ctggtcgagc acctcaaccc gacggcgctg gaatcgttcc cgggctggct
     8821 gcacgacggg gacgtcgtca cgctgcgggt cgaggggctg ggggagacac ggcaagtcgt
     8881 gcgcgccagc cgtgcgccgc acccactggc cgcccgtccc aacccgagcg ccaaaacggc
     8941 ggcggcacga gtcaaccggg cgccagcgcg ggtgccctac acccgcgggc tccatgaggt
     9001 cgccgagcgg gtgtgggcgt ggacgttgcc cgacggcggc tacggctgga gcaacgccgg
     9061 actggtcgcc ggcgacggcg cctcgctcct ggtcgacacg ctgttcgacc tggcgctgac
     9121 ccgcgagatg ctcgccgcga tgaagg
//
LOCUS       DASSGQ010000043         7374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_884047,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000043 DASSGQ010000000
VERSION     DASSGQ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884047"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..417
                     /locus_tag="VFI55_01970"
     CDS             31..417
                     /locus_tag="VFI55_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET7072835.1"
                     /translation="MAPTFKDVITSKYLLLTTFTKDGRPKPTPIWGVPEGGKLLVITD
                     DGSWKVRRIRNTPSVTVAKSGSLGKPKSDPVEGVARFLPRSETRRVYNAVLKRYWYHA
                     WWFYAHSLVRGGIDKVHIGLEIEPAA"
     gene            complement(440..1417)
                     /locus_tag="VFI55_01975"
     CDS             complement(440..1417)
                     /locus_tag="VFI55_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7072836.1"
                     /translation="MRVLVTGGTGFVGGWTAKAIADAGHSIRFLVRNPDRLATSVAKL
                     GVDVSDFAVGDIADRVSVRDALRGCDAVVHSAALVATDPRQAAQMLTTNMQGAQNVLG
                     QSVELGLDPIIHVSSFTALFHPGLEMLRADLPVVGGADGYGRSKAQVDIYARGLQDAG
                     APVNITYPGMVLGPPVGDQFGEAGEGVKAALQMHAIPGRSAAWLVVDVRDLAALHAAL
                     VEPGRGPRRYTAGGHRVPAAELANLLGQIAGTPMIAVPIPDTALRVAGAVMDRAGRFL
                     PFDTPFTSAGMQYYTQMPASDDSPSERELGITYRDPRETVADTFEALRG"
     gene            complement(1437..2051)
                     /locus_tag="VFI55_01980"
     CDS             complement(1437..2051)
                     /locus_tag="VFI55_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HET7072837.1"
                     /translation="MPAAAADPCPDVEVVFARGSGEPPGVGGVGGSFVDSLRGQIGGR
                     AMGVYPVNYQASNDFDNPDFPATVIDGIRDASSHIESMAANCPNTREVLGGYSQGAAV
                     AGFVTSAAVPPGVPAAAVPKPMSPDIANHIAAVTLFGTPSDQFLKKYGAPTIAIGPLY
                     QPKTLELCASGDPVCGDGSSPPAHAAYAVNGMTGEAANFAASHL"
     gene            complement(2307..2480)
                     /locus_tag="VFI55_01985"
     CDS             complement(2307..2480)
                     /locus_tag="VFI55_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET7072838.1"
                     /translation="MSGADKFKNKIEDLGGRAKEAIGKATGDAETRNEGRADQVKSHL
                     KDAGEKVKDAFKK"
     gene            2597..2749
                     /locus_tag="VFI55_01990"
     CDS             2597..2749
                     /locus_tag="VFI55_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072839.1"
                     /translation="MSTAVVVGIVATLMLLGIGMVVNQLLRLRGFLKQSPPGNPPDEE
                     DRQPPQ"
     gene            2818..4119
                     /locus_tag="VFI55_01995"
     CDS             2818..4119
                     /locus_tag="VFI55_01995"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7072840.1"
                     /translation="MKPSTLLITDDGLPPGVSGAADPHFANVIKIFLRLFPGRRFGGG
                     ALSVYIDGIPVVDVWTGWSDRAGTQRWTADTGAMVFSATKGVASTVIHRLADRGLVSY
                     DVPVAEYWPEFAANGKADITVRDVLRHRSGLSHLRGVTKTELMDHLLMEQRLAAAPVD
                     HLRGVQAYHALTYGWLLSGLARAVTGKGMRDLIRQEVARPLNTDGLHLGRPPEDAPTK
                     VAEILIPQAKLRTPVINFIAPRLAGLPFSGALGAMYFPGVVSLIQGDTALLDGEVPAA
                     NGVVTGRGLAKMYAALANDGRIDGKKYLSKELVRGLTGQPRRKWPDANMVVPMPFHLG
                     YHESPVPGLLNGFGHVGLGGTLGWADPEFGSAFGFIHNRLVTPLLFDMGSFAGLAGPL
                     RNAIEAARHRGPLQVPRLGAAYPARPSARARAAASAGDRRM"
     gene            complement(4064..5122)
                     /locus_tag="VFI55_02000"
     CDS             complement(4064..5122)
                     /locus_tag="VFI55_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HET7072841.1"
                     /translation="MPNNYRVVQWTTGNVGKSSLQSIAENPALELVGCYAWSPEKAGR
                     DAGELVGIAPLGVTSTNDVDALLALKPDCVVYNPMWINVDELVRILSAGVNVVTTASF
                     ITGENLGGGRDRIAEACHKGGSTMFGSGVSPGFAELLAIVSAMVCNRIDKVSVNEAAD
                     TTFYDSPDTEKPVGFGQPIDHPQLQQMAAKGTAIFAEAVRLVADALGVELDDVRCVAE
                     FAQTTADLDLGSWTIAAGCVAGVYVSWRGVIGERTVIDLNVRWRKGQNLEPDWKIDQD
                     GWVIQVDGQPSVTTKVGFLPPPYFQAETIADFMALGHIMTAMPTINAIPAVIAAPPGI
                     VTYADLPLTLPRGHVPTA"
     gene            complement(5151..6314)
                     /locus_tag="VFI55_02005"
     CDS             complement(5151..6314)
                     /locus_tag="VFI55_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072842.1"
                     /translation="MRLVVDSGLWSTGPTIATPPPLVVVLEVSGAVLSWTVDDPPGDA
                     ATRITFTDLSRADWLWRVLGASGHVALAATLGADPNTDRPDGIELAGVDIVAGSVEPL
                     RRLAVGHWLRRWWPASRQDGIAGLDRALLDVEVALLTVGAQDFFTDDTLDSDVAELLA
                     PHAASLIMHIRDDDPRIRDLAVAGSGLADDIGVDDTGWPELFAALDDSRAVLTMPTGR
                     RDDYALAAGSGVGLGHQGDAAPIARGVASITWSAVPPRIFDAAENTVDWSVQVTGAAV
                     VALVRAAVIGPDSPTGIEVRLRSGPITGTGALDGDGRATLPLVDAGHRPVTESAAWDH
                     DWTNASVIIGAAVPGVTETRETRERVRRWVRGRLDRPGGDAFLAEILAAESDY"
     gene            complement(6311..>7374)
                     /locus_tag="VFI55_02010"
     CDS             complement(6311..>7374)
                     /locus_tag="VFI55_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072843.1"
                     /translation="AWFEILKLERRRQRIRTGNAQELYFPVCFELAVTKGPPAPHDHQ
                     TPAAALADIHRGRDRTGIEALHEYVANPEVVARLADQLDRSWRDVRPGVTGENLADPF
                     LAELATVLGPANSHRTAVARQRVWSAVIADATPYNLGALARSEGPETAALPWSIVELG
                     LSSIEPQRQPTISIGSDSDRPLDRSVVDRVRATLRRALDRDALPDIPLLCEEEVDRAC
                     APWGLLSEDKQAALVAGIEVAVELAPLDRSVSTRYALAAQIQARLRKEAYVLHARRYL
                     AEGGPIHPRQRQVVDDLTAYAHPYLSRLWARLHGRDVWQEPCDDVDEVRSLLEGVARS
                     VSLDHRQRIKAMLELQVAG"
BASE COUNT         1147 a         2524 c         2449 g         1254 t
ORIGIN      
        1 agcaggcacg gcccgggcat ttgcagtggc gtggctccca ctttcaagga cgtcatcacg
       61 tccaagtacc tgcttttgac gacgttcacc aaggatggcc gtcccaagcc gacaccgatc
      121 tggggagtgc ccgagggcgg caaactcctc gtcatcaccg atgacgggtc gtggaaggtc
      181 cgccggatcc gtaacacgcc cagcgtcacc gtcgccaaga gcggatcgtt gggcaagccc
      241 aagagcgatc cggtcgaagg ggtcgcccgg ttcctgccca ggtccgagac ccgccgggtc
      301 tacaacgccg tgctcaagcg gtactggtac cacgcctggt ggttttatgc gcactcgctg
      361 gtgcgcggcg gcatcgacaa ggtgcacatc ggcctggaga tcgaacccgc ggcgtagaaa
      421 ctgacggggg gctacccggc tatccgcgaa gcgcctcgaa tgtgtcggcc accgtttccc
      481 ggggatcccg gtaggtgatg ccgagttcgc gctcgctcgg cgaatcgtcg gacgccggca
      541 tctgggtgta gtactgcatc cccgccgagg tgaagggcgt gtcgaagggc agaaatcggc
      601 ccgcccgatc catcaccgcc cccgcgacgc gcagcgcggt gtcgggaatc ggaacggcga
      661 tcatgggcgt cccggcgatt tggccgagca ggttcgccaa ttcggcggcg ggaacccgat
      721 gaccacccgc ggtgtagcgg cgtggcccgc ggccgggctc caccagcgcc gcatgcagag
      781 cggccagatc gcggacgtct acgaccaacc atgccgcgct tcgccccggg atcgcatgca
      841 tctgcagcgc cgccttgacc ccctcgccgg cctcaccgaa ttggtcgccg accggcggac
      901 cgagaaccat gcccggatag gtgatgttga ccggcgcgcc ggcgtcctgc aggccgcggg
      961 cgtagatgtc gacctgcgcc ttggaccttc cgtacccgtc cgccccaccg accaccggca
     1021 ggtccgccct cagcatctcc aggcccggat gaaacagcgc ggtgaagctc gacacgtgga
     1081 tgatcggatc caggccgagc tcgacggact gtccgaggac gttttgtgct ccctgcatgt
     1141 tcgtggtcag catctgggcg gcctgacgcg ggtcggtcgc caccagcgcg gcactgtgca
     1201 cgacggcgtc gcagccgcgc aacgcgtccc tcaccgagac gcgatcggcg atgtccccga
     1261 cggcgaagtc cgacacgtcc acacccagtt tggcgacgga ggtcgccagc cgatcgggat
     1321 tgcgcaccag aaaccggatg gagtgcccgg cgtcggcgat ggccttggcg gtccacccgc
     1381 ccacgaatcc agtgccgccc gtgaccagaa ctcgcatggt gcgattctgc aacgaactac
     1441 aggtgactcg cagcgaagtt cgcggcctcg cccgtcatgc cattgaccgc atacgcggca
     1501 tgcgccggcg gactgctgcc gtcaccgcag acagggtcgc cggaagcaca caattccagg
     1561 gtcttgggct gatacagcgg gccgatcgcg atcgtcgggg cgccgtactt cttgaggaac
     1621 tggtcggaag gtgtcccgaa cagcgtgacc gcggcgatgt ggtttgcgat atcaggcgac
     1681 atcggctttg gcaccgcggc ggcgggtacg cccggtggta cggcagccga cgtgacgaag
     1741 cccgcgacgg ccgcgccctg ggagtatccg ccgagcactt cccgggtatt cgggcagttc
     1801 gcggccatcg actcgatgtg cgagctcgcg tcacggatcc cgtcgatcac cgtcgccggg
     1861 aaatcgggat tgtcgaagtc gttactcgcc tggtagttca ccgggtatac gcccatcgcc
     1921 cggccgccga tctgcccacg caacgagtcg acgaacgatc cgccgacccc gccgacgccg
     1981 gggggctcgc cgctaccgcg agcgaacacc acttcgacgt ccgggcacgg atcggcggcc
     2041 gccgccggca tcggcgcgct cagcagccca aaggtcgcga ccagcgccgc gactccgagt
     2101 ggcgggccga gccggcgcgc ccgcgcagac ccgatccgtg cccgcgcaaa cccgatcaat
     2161 ctggcgacgt tgatttcgtg gatttccatg cacccgcctc gcatcgttgg gttcccttgc
     2221 tggtattcga cgttaatcgg ggcttggatt gccagtcgga tattttccaa ctacacggtg
     2281 ttgtcgcgtg cttggttcac gcgcgattac ttcttgaagg cgtcctttac cttctcgccg
     2341 gcatctttca ggtgggactt cacctgatcc gcccggcctt cattcctggt ctcggcgtct
     2401 ccggtggcct tgccgatggc ttctttggcc cggccgccca ggtcctcgat cttgttcttg
     2461 aacttgtccg cgccgctcat gggttcctgc ctcctcctga ttgctgtgtt gtgcttgttc
     2521 taactacccg gcggcggacg atgtcgaaac cctggcgatc cgctattcgg ccgatcggcc
     2581 tgtcatgatg gaatttgtga gcaccgcagt cgtggtggga atcgtggcga cgctgatgtt
     2641 gctgggcatc ggcatggtgg tcaatcagct ccttcggctg cggggatttc tgaagcagtc
     2701 gccccccggc aacccgcccg acgaggaaga ccgccaaccc ccgcagtgat cgaccactga
     2761 agattgtgta cggggttctc ctttcctgat aacgtcattc tcatgacggc agcgcgagtg
     2821 aaaccgtcga ctctgctgat aaccgacgat ggcttaccgc ccggtgtctc cggggctgcc
     2881 gatccacatt tcgcgaacgt catcaagatc tttctgcggc tgtttcccgg ccgccggttt
     2941 ggtggcggcg ccctcagcgt ttacatcgac ggcatacccg tcgtcgacgt ctggacgggg
     3001 tggtcggatc gggccggcac ccagcgctgg acggccgaca cgggcgccat ggtgttctcg
     3061 gcgaccaagg gtgtggcgtc cacggtgatt caccggctgg ccgaccgggg cctggtgtcc
     3121 tacgacgtcc cggtcgccga gtactggccc gaattcgcgg ccaacgggaa ggcggacatc
     3181 acggtccgcg acgtcctgcg gcaccgatcc gggttatcgc acctgcgggg cgtcaccaag
     3241 accgagttga tggaccacct cctgatggag cagcggctgg ccgccgcgcc cgtcgaccat
     3301 ttgcgcggcg tgcaggccta ccacgcgctg acctacggat ggctgctgtc cggtctggcc
     3361 agggctgtga ccggcaaggg catgcgcgac ctgatccgcc aggaggtcgc gcggccgctc
     3421 aacaccgacg gcctgcatct cggccgtcca cccgaagacg cgccgaccaa ggtggcggag
     3481 atcctgatac cgcaggcgaa attgcgcact ccggtcatca atttcatcgc gccgaggctg
     3541 gccggcctgc cattctcggg cgccctgggc gccatgtact ttcccggcgt cgtatccctg
     3601 atccagggcg acaccgcgct tttggatggc gaggtgcccg cggccaacgg tgtggtgacg
     3661 ggccgtggtc tggccaagat gtatgccgcg ctggccaacg acggccgcat cgacggaaag
     3721 aagtatctgt ccaaggagtt ggtgcgcggt ctcaccgggc agccgcgacg taagtggccc
     3781 gacgcgaaca tggtggtgcc catgcctttt cacctgggct atcacgaatc gccggttccc
     3841 ggcctgctca acgggttcgg tcacgtcggt ctgggtggca cgctcggatg ggccgacccc
     3901 gaattcggca gcgccttcgg gttcatccac aaccggctgg tgacgccgtt gctgttcgac
     3961 atgggatcgt tcgcgggcct ggccggtccg ctgcgcaatg cgatcgaggc ggcgcgtcac
     4021 cggggtccgc tacaggtgcc gcggctcggt gcggcttacc cggctaggcc gtcggcacgt
     4081 gcccgcgcgg cagcgtcagc gggagatcgg cgtatgtgac gatgcccggc ggcgcagcga
     4141 tgacggccgg gatcgcgttg atggtgggca tcgccgtcat gatgtgcccg agcgccatga
     4201 agtcggcgat ggtctcggcc tggaaatacg gcgggggcag gaagccgacc ttggtcgtca
     4261 ccgacggctg cccgtcgacc tggatgaccc agccgtcctg gtcgatcttc caatcgggtt
     4321 ccaggttctg tcccttgcgc caccggacgt tgaggtcgat cacggttctt tcgccgatga
     4381 cgccccgcca gctgacgtag acgcccgcca cgcatcccgc ggctatcgtc catgacccca
     4441 ggtcgagatc ggctgtggtc tgggcgaact cggccacgca ccgcacgtcg tcgagctcca
     4501 caccgagcgc atcggccacc agccggacgg cctcggcgaa aatggccgtg cccttggccg
     4561 ccatctgctg cagctgcggg tggtcgatcg gctggccgaa gccgaccggc ttctcggtgt
     4621 cgggtgagtc gtagaacgtg gtgtccgcgg cctcgttgac ggagaccttg tcgatccggt
     4681 tgcacaccat cgccgacacg atggccagca gctcggcgaa gccggggctg acgccggacc
     4741 cgaacatcgt cgacccgcct ttatggcagg cttccgcgat ccggtcgcga ccgccgccca
     4801 ggttttcccc ggtgatgaac gacgcggtcg tcaccacgtt gacgcccgcg gacaggatgc
     4861 gcaccagctc gtcgacgttg atccacatcg ggttgtagac gacgcagtcc ggtttgaggg
     4921 ccagtagcgc gtcgacgtcg ttggtggaag tcacgcccaa cggcgcgatg ccgaccagtt
     4981 cgccggcgtc gcggccggct ttctccggcg accaggcgta acagcccacc agttccagcg
     5041 cggggttttc ggcgatcgat tgcaatgagc tcttgccgac gttcccggtg gtccactgga
     5101 cgactcgata gttgtttggc actcgctcag cataggggct cgatcggagc tcagtaatcg
     5161 gattcggcgg ccaagatctc ggccaggaat gcgtcgccgc ccgggcggtc cagccgcccc
     5221 cgcacccacc ggcgcacacg ctctcgcgtt tcgcgtgtct ccgtcacgcc cggcaccgcg
     5281 gcaccgatga tcaccgaggc gttcgtccaa tcgtggtccc aggccgccga ttccgtcacg
     5341 ggccggtgcc ccgcgtcgac cagggggagg gtcgcgcggc cgtcgccgtc cagggcgccc
     5401 gtgccggtga tgggtccgga ccgcagccgc acctcgattc ccgtgggcga atccggcccg
     5461 atgacggctg cccgcacgag ggcgacgacc gccgcaccgg tcacctggac gctccagtcg
     5521 acggtgttct cggcggcatc gaagattcgg ggagggaccg cgctccaggt gatggacgcg
     5581 acgccgcggg cgatcggagc cgcgtccccc tggtggccga gtcccacgcc ggaaccggcg
     5641 gcgagcgcgt agtcgtcccg gcgccccgtc ggcatcgtca gaaccgctct cgaatcatca
     5701 agagccgcga acagttccgg ccatcccgtg tcgtcgacgc cgatgtcgtc ggcaagcccg
     5761 ctaccggcca ccgccaggtc gcgtatccgc ggatcgtcat cccggatgtg catgatcagg
     5821 ctggcggcat gcggtgcaag caactcggcg acatccgaat ccagcgtgtc gtcggtgaag
     5881 aagtcctgcg cgccgacggt caacagggcg acctcgacgt cgagcagggc gcgatcgagg
     5941 cccgcgatac cgtcctggcg actggcgggc caccaccgcc gcagccaatg gcccacggcc
     6001 aggcgccgga gcggctcgac cgatccggcg acgatgtcga cgccggcgag ctcgatgcca
     6061 tccggtcggt cggtgttggg gtccgcgccc agcgtcgccg ccagggcgac gtgtcccgat
     6121 gcaccgagga cacgccaaag ccagtcggcg cgggacaaat cggtgaaggt gatccgggtt
     6181 gccgcgtcgc cgggcggatc gtccaccgtc caggacagca ccgcgccgct gacctccagc
     6241 accacgacca acggcggggg agtcgcgatg gtggggccgg tactccaaag accggaatcg
     6301 acaaccaatc tcatccggcc acctgcaact ccagcattgc cttgatccgt tgccggtggt
     6361 caaggctcac cgaccgcgcg actccctcca gcagcgaccg cacctcatcc acatcgtcgc
     6421 agggctcctg ccagacgtcg cgtccgtgca gtcgcgccca cagcctgctc aggtaggggt
     6481 gtgcgtacgc ggtgaggtcg tcgactacct gccgttggcg tggatggatc gggccgccct
     6541 cggcaagata gcgccgcgcg tgcagcacgt aggcctcctt gcgcagcctg gcctggatct
     6601 gcgccgccaa cgcgtaccgg gtggaaacgg atcggtccag cggtgccagc tcgaccgcca
     6661 cctcgatgcc ggcgaccagc gcggcctgct tgtcctccga caacagcccc cagggtgcgc
     6721 aggcccggtc cacctcctct tcgcacaaca ggggaatatc gggcagcgcg tcgcgatcca
     6781 gggcccgacg cagtgtcgcg cgtactcggt ccaccacgct gcgatccagc ggccggtcgc
     6841 tatcggagcc gatcgagatc gtcggctgcc gttgcggctc aatcgaactc aatcccaact
     6901 cgacgatcga ccacggtagc gcggcggttt ccggcccttc cgagcgggcc agtgcgccga
     6961 ggttgtacgg tgtcgcgtcg gcgatcacgg ccgaccagac ccgctgccgg gccaccgcgg
     7021 tgcgatggct gttggccggc cccagcaccg ttgcgagctc ggcgaggaaa gggtcggcga
     7081 ggttttcgcc ggtcacgccg ggccgcacgt cacgccaact gcggtcgagc tggtcggcga
     7141 gccgggcgac gacctcgggg ttcgccacgt attcgtgcaa cgcctcgatc ccggtgcggt
     7201 cccggccccg gtggatgtcg gcgagcgccg cggccggcgt ctggtgatcg tgcggtgccg
     7261 gtgggccttt cgtcaccgcg agctcgaagc acaccgggaa gtacagttcc tgggcgtttc
     7321 cggtgcgaat ccgctgcctg cggcgctcca gtttgaggat ctcgaaccat gctg
//
LOCUS       DASSGQ010000044         8661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_550047,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000044 DASSGQ010000000
VERSION     DASSGQ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8661
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550047"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2557)
                     /locus_tag="VFI55_02015"
     CDS             complement(<1..2557)
                     /locus_tag="VFI55_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7072844.1"
                     /translation="MRPEASAFRAAPLTVWVGPTRYVFAPGRDVLVGYGRGVDISLGP
                     PGNAGPPAPRPDVVLRFTGSHWVAIDRSPGGMFVDGARVPTLDIRSGQAIAIGDPQRG
                     PRLVFQLGPPAGPPGRHPGPPQRPAQPPRQPPRSPRPPQPPQSPPRPQQLPPRPQQLP
                     PPAPPPVAAQPNRPNPPNVHVPTQRATQRLQVAPPAPPAAPAAERPGQTTRSGPTASP
                     PIPPIPLPQEPPAAEDEQSKGRTLIERMTTRRIRTPPPAARTEDPEDPNSTYRLPLKP
                     GARTTGMTAYQLRLTADGREVLTDISFKARPASLTAVVGPSAARNSALLGLLAGRREQ
                     SSGRITVDGHDVRAEPESMRSRIGIVLREERDVCIHPRLTVEQALGYAAELRLPPDTA
                     PEHRRRVVDQVLDELDLTAHRATRISKLSPEVRRCASIAIELITRPTLLVVDEPNAGL
                     DAAQQHHVMTILRRQADIGCAVVVTKSSGSSLAGVDMCDQVLVLTSTGTVAFLGAPPQ
                     IESSMGTTDWSKVLEQVGADPDGAHRAFRARPPASGPGAPPEVAAPWPLPAELPVKRQ
                     IGLLIRRQLRLLFADHVYLLFLAVLPFVSAALTLLIPGDSGLDRPKPSSPNLHEAIEV
                     LAALNIAAVIVGSALTIRTVVEERRIFRREQAVGLSTFAYVAAKMIVFGLAAAVLTAI
                     LFAVVIAVKGGPVHGGVLLHGVRMGATIELYLGVAATAVVSTIVGLALSSLGNRPREV
                     VPLLVPVILASALFNGSLVELVGTWGLQQISWFVPAQWGFAATASTVDLRRVDALAAN
                     AEMWTHYSGWWLFDMVMLVLLGAGVAGFVLYRLRTPDAAVRRRALRHQPGDLSH"
     gene            complement(2652..3149)
                     /locus_tag="VFI55_02020"
     CDS             complement(2652..3149)
                     /locus_tag="VFI55_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020786820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET7072845.1"
                     /translation="MNASAANHAEVVDAANSEPQSGLGADLLAVVARLNRLATQRIQM
                     PLPAAQARLLATIEAHGEARIGDLAAVDHCSQPTMTTQVRRLEDAGLVTRTVDPGDAR
                     AVRIRITDQGIRTLNAVRADRAAAIEPQLGLLEPADRQVLKEAVEVLRRLLENAATTG
                     RRNTL"
     gene            3268..5325
                     /locus_tag="VFI55_02025"
     CDS             3268..5325
                     /locus_tag="VFI55_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7072846.1"
                     /translation="MTVPRPLPSRHRGGPLQPVELAQASVMGALCAVVAIISVIVPFA
                     AGLALLGTVPTGLLAYRYRLRVLVAATVAAGVIAFLIAGLGGFMTVVHCVYIGGLTGF
                     IKRRGRGTGTVIVSSLIAGVAFGAVNIGALMVMTRLRHLIFEVTSANVNGLATFLSRV
                     HMQGVAADLKRYFADAVHFWPWLLLAYFAIGIMIVSLIGWWALSRLLERLRGVPDVHK
                     LEAWEGSKGEERPIGPVPVRLDKVRFRYPGANQDALRELSLDLRVGEHVAITGPNGSG
                     KTTLMLILAGRKPTSGTVHRAGAVGLGKLGGTAVVLQHPESQVLGSRVADDVVWGLPP
                     GTEIDVARLLGEVGLDGLAERDTGSLSGGELQRLALAAALAREPALLIADEVTTMVDQ
                     QGREALLGVLSGLTERHRTALVHITHYNNEADSADRTIDLSDSPDNADMVEIAQAPAP
                     TTVVDHSQDAPVIELLNVGHEYGSGTPWAKTALRDVSFTVEQGDGVLIHGGNGSGKST
                     LAWIMAGLTIPTTGSCLMDGRPTHEQVGAVALSFQAARLQLMRSRVDLEVASAAGFSH
                     RDADRVAAALAVVGLDPTLAKRRIDQLSGGQMRRVVLAGLLARSPRALILDEPLAGLD
                     ATSQRGLLRLLEDLRRDRGLTVVVISHDFVGLDDLCPRILHLRNGTMEPLKPMPAAAG
                     GVA"
     gene            5322..6170
                     /locus_tag="VFI55_02030"
     CDS             5322..6170
                     /locus_tag="VFI55_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET7072847.1"
                     /translation="MSTTPAPAQDTRRRPRPVVLLVPVPGTSAIHKLWAGTKLLVVFG
                     VSLLVTFYPGWVTIGLVAALVFAAVRIARIPRGVLPSVPRWLWVVIALGGITAALAGG
                     SPNVSIGGLQIGLGGGLNFLRITALSVVLLALGAMVSWTTNVAEIGPALATLGRPFRA
                     LRIPVDEWAVALALALRAFPMLIDEFQVLYAARRLRPKRIPHSRKARRRRHALELIDL
                     LAAAITVTLRRADEMGDAITARGGTGQLSANPAQPKLADWVTLAIVIATSGAAVVLET
                     ILHTAH"
     gene            6210..6659
                     /locus_tag="VFI55_02035"
     CDS             6210..6659
                     /locus_tag="VFI55_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7072848.1"
                     /translation="MADIHRTRTIAARVGEIWDVLADFGSISSWAGNVDHSCILFSGA
                     DGKPLGTARRVQIKRDALVERITEFDPPHVLAYDIDGLPRRLRRVANRWTLTAEGEST
                     VVALTSTVEIGPRPDQKLAERVLCRFLARQSDAMLAGLANRLENARV"
     gene            6712..8499
                     /locus_tag="VFI55_02040"
     CDS             6712..8499
                     /locus_tag="VFI55_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET7072849.1"
                     /translation="MTDEERAVPPYEAADVAAWRDRMLTGRKWFDEHGVGFGRHYTGS
                     LACVPSRPTIFTGHYPDLHGVTQTDGIGKTADDTRMRWLRQGEVPTLGNWFRAAGYDT
                     HYDGKWHISHADLIDPGTGRPLLTNDDDGVVDSAAVRRYLDADPLAPFGFSGWVGPEP
                     HGALLANAGIRRDPLIADRVVAWLTDRYARRRAGDEAALRPFLLVASFVNPHDIVLFP
                     GWSRRSPVKPSPLDPPPVPPAPTADEDLSTKPAAQIAFREAYYSGYGPAPAINWTYDH
                     NAQRYRDLYYRLHAEVDGPIDRVRRAVTEGGSDNAVLVRTADHGDLLGAHGGLHQKWF
                     NLYDEATRVPFVIARIGERPTMPRVVTAPTSHVDLIPTLLGAAGFDASGVAALATTLA
                     ESFSEVHELPGRDLMPVVDGAPADDARAVYILTRDNMLEGDSGASGLARLLKRTVNPP
                     APLRIRVPAHTASNFEGLVVRVDEASATGGAGHLWKLVRTFDDPATWTEPGVRQLAAN
                     GLGGEAYRTSPLDDQWELYDLTADPLEADNRWTDPDLDDLRRHLRTRLKHVRADSVPE
                     RNQPWPYASRRPPSPSTSRPWRRFGKPRT"
     gene            complement(8575..>8661)
                     /locus_tag="VFI55_02045"
     CDS             complement(8575..>8661)
                     /locus_tag="VFI55_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET7072850.1"
                     /translation="LERIRSSADIERSESIVVLSNLIDRMRT"
BASE COUNT         1298 a         2964 c         3024 g         1375 t
ORIGIN      
        1 catgtgacag gtcacccggt tggtgccgca gcgcccggcg gcggactgcg gcgtcgggcg
       61 tccgcagccg gtacagcacg aagccggcca cccctgcgcc gaggagcacc agcatgacca
      121 tgtcgaacag ccaccacccc gaatagtggg tccacatttc ggcgttggcg gccagcgcgt
      181 cgaccctgcg cagatcaacg gtggacgccg tcgccgcgaa gccccactgg gccgggacga
      241 accaggagat ctgctgcaag ccccacgtgc ccaccagctc aaccagactc ccgttgaaca
      301 gcgcggaggc caggatcacc ggcacgagca gcggcacgac ctcccgtggc ctgttcccca
      361 gcgaagacag cgccagcccg acgatcgtcg agacgacggc cgtggcggcg acgcccaagt
      421 agagctcgat cgtggccccc atcctgacac cgtgcagcaa caccccgccg tgcacgggtc
      481 cgcctttgac ggcgatgacg acggcgaaca ggatggccgt caacaccgcg gcggccagtc
      541 cgaagacgat catcttggcg gccacatagg caaacgtcga cagcccgacg gcctgctcgc
      601 gccggaagat gcggcgctcc tcgaccaccg tgcggatcgt cagcgcggag cccacgatca
      661 ctgcggcgat gttgagcgcg gccaggacct cgatggcttc gtgcaggttg gggctgctcg
      721 gcttgggccg gtccaggccc gagtcgccag ggatcagcag ggtcaacgcc gccgacacga
      781 acggcaggac cgccaaaaag agcaggtaga cgtggtcggc gaagagcagg cgtagctggc
      841 gacggatcag caacccgatt tgccgcttca ccggaagctc ggcgggaagc ggccacggcg
      901 ccgcgacctc cggcggcgcg cccggaccgg acgcaggcgg gcgggcacga aacgcacggt
      961 gtgcaccgtc gggatcggca ccgacctgct cgaggacttt cgaccagtcg gtggtgccca
     1021 tcgacgattc gatctgcggg ggcgccccga ggaaggccac ggtgcccgtc gaggtgagca
     1081 ccaacacctg gtcgcacatg tcgacgccgg ccagcgatga tcccgacgac ttcgtcacca
     1141 cgaccgcaca gccgatgtcg gcctggcgcc gcaggattgt catcacgtgg tgttgttggg
     1201 ccgcatccag cccggcgttc ggctcgtcca ccacgagcag cgtgggtctg gtgatgagtt
     1261 cgatcgcgat cgacgcgcac cggcgcacct cgggcgagag tttgctgatc cgggtcgcgc
     1321 ggtgggctgt caggtcgagc tcgtcgagga cctggtccac cacgcggcgg cggtgctcgg
     1381 gtgcggtgtc cggcggcagg cgcagttcgg cggcgtagcc caacgcttgt tcgacggtga
     1441 gtcggggatg aatgcacacg tcgcgctcct cgcgcagcac gatcccgatg cgggagcgca
     1501 tcgactcggg ctcggcgcgc acgtcgtggc cgtcgacggt gattcggccg gaactttgtt
     1561 ccctcctgcc ggcgagcagg ccgagcagcg ccgagttgcg tgccgccgac ggcccgacaa
     1621 ccgcggtcag tgacgccgga cgcgccttga acgagatgtc cgtgagcacc tcgcgcccgt
     1681 cggccgtgag gcgcagttgg tatgcggtca tcccggtggt gcgcgcgccg ggtttgagcg
     1741 gcaatcgata cgtggagttg gggtcttcgg ggtcttcggt gcgggcggcg ggcggtgggg
     1801 tgcggatcct gcgcgtggtc atccgctcga tcaaagtccg accctttgac tgctcgtcct
     1861 ctgcggcggg cggttcctgg gggagcggga ttggcgggat gggcggtgac gccgtcggtc
     1921 ccgaccgggt ggtttggccg ggtcgttctg cggcgggcgc agcgggcggg gcgggcgggg
     1981 cgacctgtag tcgctgggtc gcccgctgcg tcgggacgtg cacgttgggc gggttcggtc
     2041 ggttcggctg cgcggcgacg ggaggtggag ccggcggtgg caattgctgg ggtcgcggcg
     2101 gcaattgctg gggtcgcggc ggcgactgcg ggggttgcgg cggtcggggg gaccgcgggg
     2161 gttgccgggg cggctgggcc gggcgttgcg gtggaccggg atgtcgcccg ggcggtccgg
     2221 ccggtgggcc gagctgaaaa accagccgcg ggccgcgctg cggatcaccg atcgcgatcg
     2281 cctgaccgga gcggatgtcg agcgtcggca cccgggcgcc gtcgacgaac atgccgcctg
     2341 ggctcctgtc gatggcgacc cagtggctac cggtgaaccg cagcacgacg tctggacgag
     2401 gcgccggcgg gccggcattg cccggtggac ccagcgagat gtcgaccccg cggccatatc
     2461 ccaccagcac atcgcggccc ggggcgaaga cgtacctggt cggtccgacc cacacggtca
     2521 acggtgcggc tcggaaggca gaagcctcgg gccgcatcac cgccaccttt cgtcacccgc
     2581 ggacatcgat tttctcgcga ttcaagtgtc cacccgtttc cggtggtcca gccaccggaa
     2641 acgcgggtcg atcacagggt gttgcggcgc cccgtcgtcg cggcgttctc gagcagccgg
     2701 cgcagcacct cgaccgcctc cttcagcacc tgccggtccg ccggctcgag caggcccaac
     2761 tgcggctcga tcgcggcggc gcggtcggcc cggaccgcgt taagcgtgcg tatgccttga
     2821 tcggtgatgc ggattcggac cgcgcgcgca tcgccggggt cgacggttcg ggtcaccagg
     2881 ccggcgtcct cgagtcgacg cacctgtgtg gtcatcgtcg gctgcgaaca gtgatcgacg
     2941 gcggccaggt caccgatgcg ggcttctccg tgggcttcga tggtggccag cagcctggcc
     3001 tgcgcggcgg gcaagggcat ctggatccgc tgcgtggcca gtcggttgag ccgggcgacc
     3061 acggcgagca gatcggctcc cagtccggac tggggctcgg agtttgcggc gtcaacgact
     3121 tcggcgtggt tcgcggcgga tgcgttcatg catccatggt tgcatagctt tgctaagcga
     3181 gacgaatctc acacaccgtc acgcgaaaac ccagcaagct tggtgttcta cggagacatg
     3241 ccgccggggc ctggcacaat cgataaagtg accgtcccca gaccactccc ctcacgtcat
     3301 cggggtggac ccctgcagcc ggtcgaattg gcacaggcct ccgtgatggg ggcgctctgt
     3361 gcggtggtcg ccatcatctc cgtgatcgtc ccgttcgcgg cgggcctggc cttgctgggc
     3421 accgtgccga ccgggctgct ggcctaccgc taccggttgc gcgtgctggt ggccgcgacc
     3481 gtcgccgcgg gggtgatcgc cttcctgatc gccggcctcg gcggtttcat gaccgtcgtc
     3541 cactgcgtct acatcggcgg tctgaccggg ttcatcaagc gcaggggccg cggcaccggc
     3601 accgtgatcg tctcgtccct gatcgccggg gtcgcgttcg gcgccgtcaa tatcggtgcg
     3661 ctgatggtga tgacccggct gcgacacctg attttcgagg tgacgagcgc gaacgtgaac
     3721 gggctcgcta cgtttctgtc ccgggtccac atgcaaggcg tcgccgccga tttgaagcgt
     3781 tatttcgccg acgcggtcca cttctggccg tggttgctgc tggcctactt cgccatcggg
     3841 atcatgatcg tgtccctgat cggctggtgg gcattgtccc ggctgctgga gcgactgcgc
     3901 ggcgtccccg acgtgcacaa gctcgaggct tgggagggga gcaagggcga ggaacgcccg
     3961 atcggaccgg ttccggtgcg gttggacaag gtgcgtttcc ggtatcccgg tgccaaccag
     4021 gacgccctgc gcgagctcag cctggacctg cgggtcggcg aacacgtcgc gatcaccggg
     4081 cccaacggct ccgggaagac cacgttgatg ctgatcctgg ccggccggaa accgacgtcg
     4141 ggcaccgtgc accgcgcggg cgcggtgggt ctgggcaagc tgggcggcac cgcggtcgtg
     4201 ctacagcacc ctgaaagcca ggtcctgggc agccgggtcg ccgacgatgt ggtctggggc
     4261 ttgccaccag gcaccgagat cgatgtggcc aggttgctgg gcgaggtcgg cctcgacgga
     4321 ctcgccgaac gcgacacagg gagcctgtcc gggggtgaac tgcagcgcct cgcgctcgcc
     4381 gcggcgctcg cccgggagcc ggcgttgctg atcgccgacg aggtcaccac catggtcgac
     4441 cagcagggcc gggaagcgtt actgggcgtg ctgtcgggcc tcaccgaacg acaccggacc
     4501 gctctggtgc acatcacgca ctacaacaac gaggccgatt ccgccgaccg caccatcgat
     4561 ctcagcgatt cgccggataa cgccgacatg gtggagattg cccaggcacc cgcaccgacc
     4621 accgtggtgg atcactcgca ggacgcgccg gtgatcgaac tcctcaacgt gggccacgaa
     4681 tacggcagcg gcactccgtg ggccaagacc gcgctgcgtg acgtcagttt caccgtggag
     4741 caaggggacg gggtgctgat ccacggcggc aacggctcgg ggaagtcgac gctggcgtgg
     4801 atcatggccg gactgaccat tccgacgacg ggctcctgcc tgatggacgg ccgacccact
     4861 catgagcagg tcggcgcggt ggcgttgtca ttccaggcgg cgcggctcca gttgatgcgc
     4921 agccgcgtcg acctggaagt ggcgtcggca gcgggctttt cgcaccgcga cgcagatcgc
     4981 gtcgccgcgg cgctcgccgt cgtcggactc gatcccacgc tcgccaagcg gcgcatcgat
     5041 cagctcagtg gcggtcagat gcggcgcgtg gtgctggccg gactcttggc ccggtcgccg
     5101 cgcgccttga tcctcgacga gcccctggcg ggcctggatg caacaagcca gcgtgggctg
     5161 ctgcgactgc tggaggacct gcgccgtgac cggggtctga cggtggtggt catttcgcat
     5221 gattttgttg ggctggacga cctgtgtcca cgcatcctgc atttgcgcaa tggcaccatg
     5281 gaaccactaa aacccatgcc ggccgcggcg gggggtgtgg cgtgagcact actccggccc
     5341 ctgcgcagga cacccggcgc cgcccacgtc cggtcgtgct gctcgtcccg gtgcccggaa
     5401 cgtcggccat tcacaagctc tgggcgggca ccaaactgct ggtcgtgttc ggcgtttcgc
     5461 tgctagtgac gttctatccc gggtgggtga cgatcggcct ggtggcggcg ctggtgttcg
     5521 cggcggtcag gatcgcgcga attccgcgcg gcgtgctgcc gtcggtaccg cggtggctgt
     5581 gggtcgtgat cgccttgggc ggcatcacgg ccgcgctggc cggcggaagc cccaacgtct
     5641 cgatcggcgg cctccagatc ggcctcggcg gagggttgaa cttcctgcgc atcaccgcgc
     5701 tgtcggtcgt gttgctcgcg ctcggcgcga tggtgtcctg gaccaccaac gtcgccgaaa
     5761 taggccctgc gctggcaact ttgggccggc cgttccgagc gttgcggatc ccggtcgacg
     5821 aatgggcagt ggctttggcg ctggcgctgc gtgccttccc gatgttgatc gatgaattcc
     5881 aggtgctcta cgccgcccgg cggctacggc ccaaacggat tccgcacagc cgcaaggccc
     5941 gtcgccgtcg gcacgccttg gagctgatcg acctgctggc cgccgccatc accgtgaccc
     6001 tgcggcgtgc cgacgagatg ggcgacgcga tcaccgcccg cggcggcacc ggtcagctct
     6061 cggccaatcc ggcgcagccc aagctggcgg actgggtgac gctcgcgatc gtcatcgcga
     6121 ccagtggcgc cgcggtggtg ctcgaaacga tcttgcacac cgcccactag atgcagtacg
     6181 gccggtcgtc gttcgcgtag tttgagcggg tggccgacat tcaccgcact cgcaccatcg
     6241 cggctcgcgt cggcgaaatc tgggatgtcc tggccgactt cggctcgatc agttcgtggg
     6301 ccggcaacgt cgatcactcg tgcatcctgt tctccggtgc ggatgggaaa cccctcggca
     6361 ccgcgcggcg ggtccagatc aaacgcgatg ccctcgtcga gcgcatcacc gagttcgacc
     6421 cgccgcacgt cctcgcctac gacatcgacg gcctgccccg ccgcctgcgc agggtggcca
     6481 accgctggac gctgacagcg gagggcgagt ccaccgtcgt ggcactgacc agcacggtcg
     6541 aaatcgggcc ccgcccagat caaaagctgg ccgaacgcgt gctgtgtcgc ttcctcgcac
     6601 gacagtccga cgcgatgctc gccgggctcg caaaccgatt ggagaacgcc cgtgtctgat
     6661 gaccccggcc tgcggcgcgc tggccccagt ggccccgatg ttgtcgtcat catgaccgac
     6721 gaggaacgcg cggtgccgcc gtacgaggcc gccgacgtgg ccgcgtggcg cgatcgaatg
     6781 ctgaccggcc gcaagtggtt cgacgagcac ggcgtcggct tcggccggca ctacaccggc
     6841 tcgctggcct gtgtgcccag ccgcccaacg attttcaccg ggcactaccc cgacctgcac
     6901 ggggtcaccc aaaccgatgg catcggaaag acggccgacg acacccggat gcgctggctc
     6961 cgccagggcg aggtgcccac actgggcaac tggttccgag ccgcgggata cgacacccac
     7021 tacgacggca agtggcacat ctcgcatgcc gatctcatcg acccggggac cggccgcccg
     7081 ctgctgacca acgacgacga cggtgtcgtc gattccgccg cggtgcggcg ctacctcgat
     7141 gccgatccgc ttgcgccgtt tggcttctcg ggatgggtcg gcccggagcc gcacggggcg
     7201 ctgctggcca acgcgggcat tcgccgcgac ccgctgatcg ccgaccgcgt cgtcgcctgg
     7261 ctcacggacc gctacgcgcg ccggcgcgcc ggcgacgagg ccgcgctgcg ccccttcctc
     7321 ctggtcgcca gcttcgtgaa cccccacgac atcgtgctgt tccccgggtg gtcgcggcgc
     7381 agcccggtta agccgtcacc gctggacccg ccgccggtgc ccccggcacc caccgcggat
     7441 gaggacctct cgaccaagcc ggctgcgcag atcgcctttc gcgaggccta ctactccggc
     7501 tacggtccgg cgcccgcgat caactggacc tacgaccaca acgcccagcg ctatcgcgac
     7561 ctgtactacc ggctgcacgc cgaggtggac gggccgatcg accgcgtcag aagggccgtc
     7621 accgaaggtg ggtcggacaa cgcggtcctg gtgcgcacgg ccgatcacgg cgacctgctg
     7681 ggcgcccacg gcggcctgca ccagaaatgg ttcaacctct acgacgaggc cacccgcgtt
     7741 ccctttgtga tcgcccgcat cggcgagcgc ccgaccatgc cacgcgtcgt caccgctccc
     7801 acgtcgcacg tcgacctgat cccgacactg ctgggagccg ccggcttcga cgcctcgggc
     7861 gtcgcagcgc tggccaccac gttggccgag tcgttctccg aagtgcacga actgccgggc
     7921 cgcgacctca tgcccgtcgt cgacggggca cccgccgacg acgcgcgcgc cgtctacatc
     7981 ttgacccgcg acaacatgct cgagggcgac agcggcgcct cgggcctggc gcgccttctc
     8041 aagcggaccg tcaatccgcc tgcgccgctg cgtattaggg tgcccgccca caccgcttcc
     8101 aacttcgagg gactggttgt ccgcgtcgat gaagcgtcgg caaccggcgg cgccgggcat
     8161 ctgtggaagc tggtccgcac cttcgacgat ccggccacct ggaccgaacc cggggtgcgt
     8221 cagctggcgg ccaacgggct gggcggcgag gcttaccgga cctctccgct ggacgaccag
     8281 tgggagctgt acgacctgac cgcagatccg ctcgaagccg acaaccgttg gacggatccg
     8341 gacctggatg acctgcgccg gcacctgagg acccggctga aacacgttcg ggccgattcc
     8401 gtcccggaac gaaaccagcc gtggccctac gcatctcgcc ggccaccgag cccgtcaacc
     8461 tcgcgaccgt ggcgcaggtt tggcaaaccg cgaacgtaac gccagtgcga aaagccgacc
     8521 cgaatttcgc agtggcgtta cgctcgcgcg gcgggttggg cggccgaaag gccgctaggt
     8581 ccgcatccgg tcgatgaggt tcgacaacac gacgatgctt tcgctgcgct cgatgtcggc
     8641 gctcgaccgg atccgctcga g
//
LOCUS       DASSGQ010000045         3939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_671533,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000045 DASSGQ010000000
VERSION     DASSGQ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671533"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            525..953
                     /locus_tag="VFI55_02050"
     CDS             525..953
                     /locus_tag="VFI55_02050"
                     /inference="COORDINATES: protein motif:HMM:NF036408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5313 family protein"
                     /protein_id="HET7072851.1"
                     /translation="MTTHTTPSIAHPDPVRWLWYTFGGSLGPRYRQWVLHDNTARTRW
                     LRQAVRGTVQISPLAVLMLVVLPFRWLTGASIVLGLLLALWYSVAYINQTGERRLVKH
                     GYEPGTLALTLHDRYLRENEDGIAQYMATYRGMETGVERC"
     gene            complement(1423..2052)
                     /locus_tag="VFI55_02055"
     CDS             complement(1423..2052)
                     /locus_tag="VFI55_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET7072852.1"
                     /translation="MSDVDDTPKPVPAWVQAHGITDRDTGIAAARASRQPLIDAGANA
                     FDGGQGFSTAVALLGGLVTRGIGLHEGAVAALGADNPFAAFTTEPKSERSRRTVPLSA
                     PIVALLKVQRAAQAAERLHTGDQWTDNGLVFTTEFGRPVDPRNLLRAIEIAAGKAGVE
                     DIGVHTLRHSAAVAWLESGVHIKAVADLLGHAGTPSRFQGLPQLVPQLG"
     gene            complement(2233..2544)
                     /locus_tag="VFI55_02060"
     CDS             complement(2233..2544)
                     /locus_tag="VFI55_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072853.1"
                     /translation="MAGVNLLLQRWVHHNSRAVVATHLYQEVSSAQFEEADISEDWCI
                     DREDDGVDIATATTEIDAWLSTATDDGSTNRDLLKNPQKRSNIRKAMRSHLQYLRDVG
                     E"
     gene            2677..>3939
                     /locus_tag="VFI55_02065"
     CDS             2677..>3939
                     /locus_tag="VFI55_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HET7072854.1"
                     /translation="MSYLSAAPEFLASAATDLAGIGSAVSAANAAAVAPTTGVLAAAD
                     DEVSAAVASLFSSHGQAFQQLGAQAAAFHAQFVRALNTAEGAYTATEAANASPLQTVW
                     QDVRAVINAPTNALLGTPLIGNGTNGAPGTGQPGGPGGLLWGNGGMGGSGAPGQPGGR
                     GGSAFLFGNGGQGGAGGAGTNGAMGSAASSTTGGSGGTGGVGGAGGNGGLIFGNGGAG
                     GVGGVGGLGGAGGGGVNGSITGASLNGGNGTNGGNGGAGGAGGAGGQGARMFGGHAGA
                     NGDGGAGGNGGQGGQGGLGATDTGTGAGNGGNGANGGSPGLGGNPGGTGALAGAAGSA
                     GNGGNGGNGGQGVIFNAGAGGNGGNGGDVTSFTGGTGGTGGNGGDGGIINVNVNATLS
                     GGAGGNGGAGGNVTGGTGGNGGNGGNGGN"
BASE COUNT          634 a         1248 c         1385 g          672 t
ORIGIN      
        1 agcaccgctg cctgggtgga agggttgcgg gtggacgaga tagcggtgga cgaagtggac
       61 gccaaccggc gctcgccact gagccgacgc aacagaagac ccccgttttg aacgtcgagg
      121 cggggttcca acaccattca atgaaggtaa ccaacttgag gaaaacccta gctgctgtcg
      181 ccgtggctgc gggagccatg ctgattgccc ctctcgcata tatgctgaca gcgactacga
      241 cggtttcgcg gcggcggcca aagcggcagg catcccgttc gtggacactg atgccatgca
      301 gcaggtttcg gcagatgagt gtggacgcat gcatcgtggc ctgccaccag tcccatccgg
      361 gcccctgcgc cgtctcccaa gcgcaggcgt aagaagcgct tcgcggccga gtacgccctg
      421 ctgcgggagt tgggcctcgg ctagcgtcta acacaatcgg gcattgtgac cccacaacat
      481 caaggccgct actgagcgtc acacaggatg gcgacgatga gggtatgact actcacacca
      541 caccctccat cgcccacccg gacccagtgc gctggctctg gtacaccttt ggcggcagcc
      601 tcgggccgcg ctaccgccag tgggtactgc acgacaacac cgctcgcacc cgctggttgc
      661 gccaggccgt gcgcgggacg gtacagatct cgccgctcgc ggtgctgatg ctggtcgtgc
      721 tgccgttcag gtggctcacc ggggcgtcaa tagtcctcgg cctactgttg gcgctctggt
      781 actccgtggc ctacatcaac cagaccggtg aacgacgcct cgtgaagcac gggtacgagc
      841 cgggaacgct ggcgctgacg ctgcacgacc gctacctccg tgagaacgag gacgggatcg
      901 cgcagtacat ggcgacctac cggggcatgg agacgggggt tgagcggtgc tgaccgttgt
      961 gccagctgcg ccaacgaaaa gaactggcac aactggcata gcggttactg gccctcctcc
     1021 tcgcggtaga tctgggccag ccgttcgaac agctcgacca cctcgccgct gacgggcgtt
     1081 gagtcgccgt tgtcatcggg gggccggtaa caaagacggt gccctggagc acatccagcc
     1141 cctccatgct cgggtctagc ttccaccaga agtagctggc catggagttg agagccactc
     1201 cagcgagctg gacggcgttg caccagaggg tgcaagtgct cggtcgagaa ggcgtctggc
     1261 ggaccgtcga cgaggctttg gagcgtgcga atgtcttgcc cgatctcgcg cacttcgtgc
     1321 cgtccccgtc gacgtaggac agtcgcgctt cccacgttcc gttggggcgc ttgcggatgt
     1381 tgccggtgcc gttcgcagtg cgtgtgccga tcgcggcgca acttacccca attggggtac
     1441 taactggggt aagccttgaa atctcgatgg tgttccggcg tgcccgagca ggtcggccac
     1501 cgccttgatg tgcacgccgg actcaagcca ggctacggcg gcgctgtgcc gcagcgtgtg
     1561 cacgccgatg tcctcgacgc ccgccttgcc ggctgcaatc tcgattgccc gcaataggtt
     1621 ccgcggatcg acaggtcgtc cgaactcggt ggtgaatact agaccgttgt ctgtccattg
     1681 atcaccggtg tgcaggcgct cggcagcctg agctgcccgc tggaccttca acagcgccac
     1741 aatgggcgcc gacagcggca cggtgcgccg ggaccgctca gattttggct cagtggtgaa
     1801 tgctgcgaaa gggttgtccg ccccgagcgc tgcgacggct ccctcatgca gaccgatccc
     1861 gcgagtcacc aggccgccaa gcagcgctac tgcagtgctg aagccctgcc ctccatcgaa
     1921 cgcgtttgca cccgcgtcga tgagtggctg acgtgaggcg cgggctgccg cgatgccggt
     1981 gtcccggtct gtgatgccat gggcctgaac ccaggctgga acgggcttcg gtgtgtcgtc
     2041 gacgtcactc accgtcggat gttcccgtac gagggcgaca gccaaccgtg atcgatccgc
     2101 atgaatccgc aagagttgca tccgcgcggg cgtcccggcc tggggttggt aatcacgatt
     2161 gcacttccat tccagctagt ttggacaaag gggcctggat gccgccattt tctaacgcgg
     2221 cactgggggt ggtcactcgc ccacgtcgcg caaatattgg agatggctcc gcatcgcttt
     2281 tcggatgttg cttcgttttt gcggattttt gagtaagtcg cggttcgtcg agccatcgtc
     2341 ggtcgcggtc gatagccagg cgtcgatttc ggttgtcgca gttgcgatgt caacgccgtc
     2401 gtcttcacgg tcgatacacc agtcttcgct gatgtcggcc tcttcaaact gggcgctgct
     2461 tacttcttga tacagatggg tggccacaac agcccggctg ttgtgatgga cccaacgctg
     2521 tagaaggagg ttgacgcctg ccatcttgct ccttacgcga ggggtgaacg tttgcccaaa
     2581 cgctgaacta tcgggtcgga atcggcctac gctctatccg ctcgtgaagt tcatccgatt
     2641 cgccgatgtg tcgacgacct ggcggaaggt atagggatgt cgtatctttc ggcggccccc
     2701 gagtttttgg cttcggcggc aacggatctg gcgggcatcg gctcggcggt gagcgccgcg
     2761 aatgcggcgg cggtggcccc gacgaccggg gtgctggccg ccgccgacga tgaggtgtcg
     2821 gcggcggtgg cgtcgctgtt ttccagccac gggcaggcct ttcagcagct cggcgcgcag
     2881 gcggcggcgt ttcatgccca gttcgtgcgg gcgctgaaca ccgccgaggg tgcgtatacg
     2941 gctaccgagg ccgctaacgc gtcgccgttg cagaccgttt ggcaagacgt gcgagcggtg
     3001 atcaatgcgc ccaccaatgc gttgttgggg accccgttga tcggcaacgg caccaatggt
     3061 gcaccgggga ccgggcagcc cggcgggccg ggcgggctgt tatggggcaa cggcggcatg
     3121 ggcgggtccg gcgctcccgg tcagcccggc ggccgcggcg gtagcgcttt cttgttcggc
     3181 aacggcggcc agggcggggc cggtggggcc ggcaccaacg gcgccatggg ctcggcggcc
     3241 agcagcacca ccggcggcag cggcggcacc ggcggggtgg gcggggccgg gggcaacggc
     3301 gggctgatct ttggcaacgg cggtgccggc ggtgtcggcg gtgttggcgg gctcggcggg
     3361 gccggtggcg gcggtgtcaa cggctctatc accggcgcct ctctcaacgg cggcaacggc
     3421 actaacggcg gcaacggcgg ggccggcggg gccggcgggg ccggcggcca aggcgcgagg
     3481 atgttcggcg gtcatgccgg cgcgaacggc gacggcgggg ccggcggcaa cggcggccag
     3541 ggcggtcagg gcggcctggg cgccaccgac accggcaccg gcgccggcaa cggcggcaac
     3601 ggcgccaacg gcgggtcccc cggcctgggc ggcaaccccg gcggcaccgg cgccctcgcg
     3661 ggcgccgcgg gcagcgccgg caacggcggc aacggcggca acggcggtca gggcgttatc
     3721 ttcaacgccg gtgccggcgg caacggcggc aacggcggcg atgtcaccag cttcaccggt
     3781 ggcaccggcg gcaccggcgg caatggtggc gacggcggca tcatcaacgt caacgtcaac
     3841 gccaccctta gcggcggcgc cggcggcaat ggcggcgcgg gtggcaacgt cactggcggc
     3901 accggcggca acggcggcaa cggcggcaac ggcggcaac
//
LOCUS       DASSGQ010000046         3587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_793256,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000046 DASSGQ010000000
VERSION     DASSGQ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793256"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1475..1999)
                     /locus_tag="VFI55_02070"
     CDS             complement(1475..1999)
                     /locus_tag="VFI55_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013128796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HET7072855.1"
                     /translation="MNVTETSTPAADAESGAAARSSDSDLFAQSEVAADYVEGLLDIL
                     DYDGDIDEVVQGGRPVVEVVGDRLQALVGQRGATLEALQELTRLAIFRATGTPSRLLL
                     DVGGYRDARRKELVSIAKNAAERVKQHGEAVRLEPMNAFERKCVHDVINATAGVESES
                     EGVEPQRRIVVHPA"
     gene            complement(2056..3051)
                     /gene="yidC"
                     /locus_tag="VFI55_02075"
     CDS             complement(2056..3051)
                     /gene="yidC"
                     /locus_tag="VFI55_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET7072856.1"
                     /translation="MGVIYYSISWLMLTWHTVWDTLGVGEWLATNWDWILSIVFLVIT
                     IRAILFPAFVKSIKSQRAMQALQPQLTEIRTKYKGDNQRIQQETMELMRAEKANPLMG
                     CLPLLVQAPVLYGLYHVLRHLAKPDISPQYKTLYGWTVPLFNSASHARLFGAPIALSF
                     RSNAAEAAHLGGTMTAVRVVAALLIVTMIITTFLTSRQMILKTGWATDPTQLMMQRLM
                     MYGVPIGLLVSGLTFPIGVVIYWVTTNLFSLGQQFYVLRKFPPPANANGGGGTGFFGP
                     TKPKTVNGKVVKTVQPTAKNKIKDTNDAPVQAKAVTGPKVGAKPVNPKKGNAAKR"
     gene            complement(3071..3334)
                     /gene="yidD"
                     /locus_tag="VFI55_02080"
     CDS             complement(3071..3334)
                     /gene="yidD"
                     /locus_tag="VFI55_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET7072857.1"
                     /translation="MTDPVTTPTAMSPGARMLTWPIIAYRRWISPALPARCRFYPSCS
                     AYALEAVQTHGAIKGIRLAIWRLLRCQPFNAGGYDPVPPRRAH"
     gene            complement(3331..>3587)
                     /gene="rnpA"
                     /locus_tag="VFI55_02085"
     CDS             complement(3331..>3587)
                     /gene="rnpA"
                     /locus_tag="VFI55_02085"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET7072858.1"
                     /translation="DPARTDHPSARAGFVVSKAVGGAVIRNTVKRRLRHLARTEIAAL
                     PAGADVVVRALPGAATRSYTQLEIDLREGIAAAIRRGGRR"
BASE COUNT          642 a         1287 c         1123 g          535 t
ORIGIN      
        1 tcaaacgatc ggccgattag tcggcgcccc gccgtgagcc gccaacccga tccggccatg
       61 cggcccgcgc gcgagctgct gccgctccca gccgagccac tcacccgacg acggcctgcc
      121 gcaaaccctc agcgccgagc catcggttcg ctctccgccg atcccagaac gccgcgtcag
      181 ccagtgatcc attcgcgtcg ccaccgatcc ccgtaacacg gcatctaccg atcgtcccat
      241 tggcgaaccc accgccgacc tcacggaccc ggaccgatcc tggccgaacc gccggcccgg
      301 caccgagttt cgtgacgcag ccaacgaagt agcgggtaca cctcgtgacg cacggatcgc
      361 tccggccccg ccccggcagc cggctggagg cggtcacgga tccggcagtc accgccaccg
      421 cgttgccgca gagccgagcg atcatcgggc gccctcggac ggacacgtga tgaccaacgc
      481 gacgtcggtc gccggcgcga ggagttgaag ggctgatagc acccggccgc caagtcgggc
      541 gccatcgaac cgcgccatcg gccggccgtg atcggtgccg ccgtggcgtg ccgggcactg
      601 cggtcagcca cccctcgcca tccacaagaa ctcagactcg tcgccggcca ccattccgcc
      661 ggctgccaag cactcgccta cgttgagtcc cctccagcac cccgaattgg cccgaacaac
      721 acccatacct caggtaccac ctgctacgaa agtagctgac ataacgtcat tgattcccac
      781 aaatacgttg aagtccctca aggccaccgc atcgcccgcg caccgaagcg cggcgagctt
      841 ggtcacaaaa ccttcaccaa gcgtccccac aacgtctgtg ccgcggggtc accaacgttg
      901 tcggtcgcgg acgcagtttc tcggccaaga accagcgcag ccgcccgcac gcacatcgcg
      961 caggggaacg aaacagacct cgcagggtct tcagttggac acaccgacgc gccgcgctgt
     1021 gaccatcggc cccaccgcca tggagacccg cacaacggtg cccgcttcac ccaccggaac
     1081 acgccacaac ccagaacgcg ccaccaaacc ggaacacgcc accaagccga aacgtgagct
     1141 tggctcgagt taccagacac cgcgtccaag gaacccggat cagctcaaac cgcgccacgg
     1201 tgccggcaag tccgaatcca ccaaggccca agcacaagcc acgccggcaa aaccagaccg
     1261 acgcccgttt ggacccagcc gccgacgcca agcacaacaa acatcgacca ggacccaccc
     1321 gccgacgccg gacgcgacaa acccccgcca cgacccggcc gccgaccccg ccgagccgca
     1381 cggaagcgcc acccaaaatc ccaaacccca aaccccacga aaaaggcgac cggccccatt
     1441 cgggaccggc cgccgatcaa cacacctgaa ccacctacgc cggatgtacg acgatccggc
     1501 gctgcggctc gacaccttcg gattccgact ccacaccggc cgtcgcgttg atcacgtcgt
     1561 gtacgcactt gcgctcgaac gcgttcatcg gctccagccg gacggcctca ccgtgctgct
     1621 tgacccgctc ggcggcgttc ttggcgatcg acaccagttc ttttcggcgg gcgtcgcggt
     1681 agccgccgac atcgagcagc agacgtgaag gggtgccggt ggcccggaag atggccagcc
     1741 gggtcagctc ctgcagcgcc tccagcgtcg caccgcgctg gccgaccagt gcctggaggc
     1801 ggtcgccgac gacctccacc actgggcgtc cgccctgtac gacctcgtcg atgtccccgt
     1861 cgtagtcgag aatgtcgagg agcccctcga cgtagtccgc agcgacctcg ctctgcgcga
     1921 acaggtcgct gtctgagctg cgggcggcgg caccggattc ggcgtccgcc gctggcgtac
     1981 tcgtctcggt cacgttcatc tccgtttgtt tctaatggcc cccgacgccg aggccgggga
     2041 ggctcatgtg cgcggctagc gcttggccgc gttgcccttc ttcgggttca ccggcttggc
     2101 gccgaccttg gggccggtga ccgccttcgc ctgtaccggg gcgtcgttgg tgtccttgat
     2161 cttgttcttc gcggtcggct gcacggtctt cacgaccttg ccgttgacgg tcttcggctt
     2221 ggtcggaccg aagaagccgg tgccgccgcc gccgttcgcg ttcgccggcg gcgggaactt
     2281 ccgcagcacg tagaactgct ggccgaggga gaagaggttg gtggtgaccc agtagatgac
     2341 cacaccgatc gggaacgtca ggccggagac gagcagaccg atcgggacgc cgtacatcat
     2401 gaggcgctgc atcatgagct gcgtcgggtc ggtggcccag ccggtcttga ggatcatctg
     2461 gcggctggtc aggaacgtcg tgatgatcat cgtgacgatc agcagggcgg cgacgacccg
     2521 tacggcggtc atggtgccac cgaggtgcgc ggcctcggcg gcgttcgacc ggaacgacag
     2581 ggcgatcggg gcaccgaaca ggcgggcgtg cgaagccgag ttgaacagcg ggacggtcca
     2641 gccgtacagc gtcttgtact gcgggctgat gtccggcttg gccaggtgtc gcagcacgtg
     2701 gtagaggccg tagagcaccg gggcctgtac gagcagcggg aggcagccca tgagcgggtt
     2761 cgccttctcc gcgcgcatca gctccatggt ttcctgctgg atgcgctggt tgtcgccctt
     2821 gtacttggtg cggatctcgg tcagctgcgg ctgaagcgcc tgcatggccc gctgcgactt
     2881 gatcgacttc acgaacgccg ggaacaggat cgcccggatc gtgatcacca ggaagacgat
     2941 cgagaggatc cagtcccagt tggtggcgag ccactcaccg acaccgagtg tgtcccagac
     3001 cgtatgccat gtcagcatca gccaggagat cgagtaatag atcacgccca ttggtgtgtg
     3061 gctcccgtaa tcagtgtgcg cgccggggtg ggactgggtc gtacccacct gcgttgaagg
     3121 gttggcaccg cagcagccgc cagatcgcga gccggattcc cttgatcgcg ccgtgcgtct
     3181 ggaccgcctc cagggcgtac gcactgcatg acgggtagaa ccggcaccgc gccggcaacg
     3241 ccgggcttat ccaacgacgg tacgcgatga tcggccatgt cagcatgcgg gcaccggggc
     3301 tcatcgcagt gggcgtcgtc acggggtcgg tcatcggcgc ccacctcggc ggatagcggc
     3361 cgcgatcccc tcgcgaagat cgatttcgag ttgcgtgtac gagcgggtgg ccgcgcccgg
     3421 cagcgcccgc acgacgacgt ccgcgccggc cggcagcgcg gcgatctcgg tccgggccag
     3481 gtgccgcagc cggcgcttga ccgtgttacg gatcaccgca ccgccgacgg ccttggagac
     3541 gacgaatccg gcccgcgccg acggatggtc ggtgcgagcc ggatccg
//
LOCUS       DASSGQ010000047         5509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1005739,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000047 DASSGQ010000000
VERSION     DASSGQ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005739"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..325)
                     /locus_tag="VFI55_02090"
     CDS             complement(35..325)
                     /locus_tag="VFI55_02090"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HET7072859.1"
                     /translation="MAVASLVCSLLGWLCLIGPILGMIFGFVALNQIKQTGQRGRGMA
                     LAGIIIGGILIALAIGLGILRTVAKHTTNTDSSGPAAVMIVEPQAVSPILAA"
     gene            complement(656..1063)
                     /locus_tag="VFI55_02095"
     CDS             complement(656..1063)
                     /locus_tag="VFI55_02095"
                     /inference="COORDINATES: protein motif:HMM:NF025197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HET7072860.1"
                     /translation="MRKVALAAAMGVLVAAGSLVAAAAAPAAKADLDFECTPGCWGAV
                     AASPSNGEEAIRLNYRTRQKAEDAAVLWCDVDGKTNDCQVLTSGLGCLSIAEGSDGKA
                     IAGRVAPTLDAADAAALDGAGPGAKIDLHDCNG"
     gene            1445..2818
                     /locus_tag="VFI55_02100"
     CDS             1445..2818
                     /locus_tag="VFI55_02100"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7072861.1"
                     /translation="MSRHRVVIIGSGFGGLNAAKALKRADVDVTLISKTTTHLFQPLL
                     YQVATGILSEGDIAPTTRLILRKQENVRVLLGEVIAIDLKAKTVTSKLMSMTTVSPFD
                     SLIVAAGAQQSYFGNDHFATFAPGMKTIDDALELRGRILGAFEAAEVTTDPAERERRL
                     TFVVVGAGPTGVEVAGQIVELAERTLAGAFRTITPSQCRVILLDAAPAVLPPMGPNLG
                     LKTQRRLEKMNVEVQLNAMVTAVDYNGIAIKEKDKGERRIECACKVWAAGVQASALGK
                     MIAEQSDGTQTDRAGRVIVEPDLSVKGHPHVFVVGDLMSVPNVPGMAQGAIQGAKYAT
                     TLIKQTLKGQDDPANRKPFKYFDKGSMATISRYSAVAKVGKLEFAGFIAWLAWLVLHL
                     FYLVGHRNRIAALFSWVMSFLGRSRGQMAITSQMIYARVVMEAQTLGGSLAVAERAEE
                     AEQKAAG"
     gene            complement(2931..4157)
                     /locus_tag="VFI55_02105"
     CDS             complement(2931..4157)
                     /locus_tag="VFI55_02105"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HET7072862.1"
                     /translation="MTHTDGESVRTPKPLPDGVSQATIGVRGGLLRSGFEETAEAMYL
                     TSGYVYESAAVAERSFIGELDHYVYSRYGNPTVTMFEERLRLIEGAPAAFATASGMAA
                     VFTSLGALLAAGDRLVAARSLFGSCFVVCNEILPRWGIETVFVDGDDIAQWEQALSVP
                     TAAVFFETPSNPMQSLVDIAAVTELAHAAGAKVVLDNVFATPLLQQGFPLGVDVVVYS
                     GTKHIDGQGRVLGGAILGDQEYIDGPVQKLMRHTGPAMSAFNAWVLLKGLETLAVRVE
                     HSNSAAHRIAEFLERHPAVSWVRYPFLESHPQYDLAKRQMTGGGTVVTFALDCARSAA
                     KQRAFEVLDKLQLIDISNNLGDAKSLITHPATTTHRAMGPEGRAAIGLGDNVVRISVG
                     LEGTDDLIADIDQALS"
     gene            complement(4154..4576)
                     /locus_tag="VFI55_02110"
     CDS             complement(4154..4576)
                     /locus_tag="VFI55_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015292128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7072863.1"
                     /translation="MSYAGDITPLQAWKLLADNPRAVLVDVRTEAEWRFVGVPDLSSL
                     GREVVYIEWNMSNGMYNEQFADELKELVPPAAADEERPVVFLCRSGNRSIGAAEVATD
                     VGINPAFNVLDGFEGHLDAHGHRGGTGWRAVGLPWKQQ"
     gene            complement(4573..>5509)
                     /gene="purT"
                     /locus_tag="VFI55_02115"
     CDS             complement(4573..>5509)
                     /gene="purT"
                     /locus_tag="VFI55_02115"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HET7072864.1"
                     /translation="ALIAVAERGDIEVFPTPRSTRLSLDREGLRRLAADELGLPTAPF
                     WFVASVAELKAVAAHSGFPLLVRPVSGRGHSVVSGPDDVEAAWQRAVANGTGEQRPRV
                     MAETIVEIEFDVTLIVVRSEGPQGPLIEFCSPIGYRSADGVVVESWQPQQMSAAAVDA
                     AKSIAARIVKALGGRGVFGVELMINGDEVYFADVTARLHQSAWVTLRSQRLSAFELQA
                     RAILGLPLDTMMMSPGAAHVVDACRDGGALTAATLTAALAVPETDLRVFAPRSGSPDA
                     TTRLGVALATAPDVPTARDRARQVTSALNVRDSRG"
BASE COUNT          982 a         1855 c         1771 g          901 t
ORIGIN      
        1 cgccggcgca tggcccttct gaaccgctgg cgacttatgc cgccagaata ggagaaaccg
       61 cttgcggttc aacgatcatc accgcggcgg ggccgctcga gtccgtgttt gtcgtgtgct
      121 tggccaccgt cctgaggatc cccaagccga tagcgagcgc gatcagaatg ccgccgataa
      181 tgattccggc gagcgccatg ccacgacccc tctgaccggt ctgcttgatc tggttcagcg
      241 cgacaaatcc gaagatcatc cccaggatgg gcccgatcag gcaaagccag cccaacaacg
      301 agcagaccaa ggatgcgaca gccatcccgt tcattccggc gcggcgcggc ggatagccat
      361 agccgtcctc aggagatgac gggtaaccgt aaccgcccgg tggtgggggt ggataactag
      421 gcggttgttc cgtcaagggg agcctcctgg atcgagaggc catactctac tctgcaggtc
      481 acaccttgtc gcaggacggt ttcatggacc agtgctgcga tggcggagcg aatcgaaacg
      541 cgttgtgcca gaatggcgat gggcttgttt ggtggtcggt ttggatcggc aggtccggaa
      601 cacgctgact cccaacgtgt ttagcggctg ccatcagacg gcagtaacga attggtcagc
      661 cgttgcagtc gtggaggtcg atcttcgccc cgggccccgc accgtccaag gctgccgcat
      721 ctgccgcgtc cagggtgggc gctacgcgcc ccgctatcgc cttaccgtcg ctgccctccg
      781 cgatcgagag gcagcccagt cccgatgtga gcacctggca atcgttcgtc ttgccgtcga
      841 cgtcgcacca caggacggcc gcgtcctccg ccttctgccg cgtccgatag ttgagtcgga
      901 tcgcctcttc tccgttcgag ggcgacgccg cgaccgcgcc ccagcatcct ggcgtgcatt
      961 caaaatccag gtcggctttg gccgccgggg ccgctgccgc ggcaaccagc gacccggcgg
     1021 cgaccagaac gcccatcgcc gcggccagcg caactttcct catactcacg acctcagcta
     1081 cgtcggctag gttccccaag tcatcgggta acgacttcac agtcgcaggc gcagttcgac
     1141 acgacgtggg agcagactgt gaggcccgtt tgaacgatcc ggttggccga gcccggcccg
     1201 cgggcgcggg ggtgggcgcg cgggctttgc ggcgcacgca caacaaaccg atctcgtcaa
     1261 cttacccacg cacattgcgt ttgcccgctc gctgcggcga tgatgacatc caccaccccg
     1321 gcgtcgccta tcgtgtcgaa tttgcgttcg atttgatctg agcctccgac agccttggcg
     1381 ccaccggcgt gagtggcgaa aactatgaag gccagtgaca cgagaagcac cggaagggac
     1441 gagtatgtcg cgccatcgcg tcgtcatcat cggaagcggg ttcggcggcc tgaacgcggc
     1501 caaggcgctc aagcgcgctg acgtcgacgt caccctgatc tcgaaaacaa ccacccacct
     1561 gttccagccg ctgctgtatc aagtggcgac cggaattctg tccgaaggcg atatcgcccc
     1621 gaccacgcgg cttatcttgc gtaagcagga gaacgtgcgg gtgttgctgg gcgaggtgat
     1681 tgccatcgac ctcaaggcca agacggtcac gtcgaagttg atgagcatga cgacggtgtc
     1741 gcccttcgac agcctcatcg tggccgccgg cgcgcagcag tcctacttcg gcaacgacca
     1801 cttcgccacc ttcgcgcccg gcatgaagac catcgatgac gcccttgagc tgcgcgggcg
     1861 catcctcggc gcgttcgagg ccgccgaggt caccaccgac cccgccgagc gtgaacgccg
     1921 gctgaccttc gtcgtggtcg gcgccggacc gaccggcgtc gaggtggccg gacagatcgt
     1981 cgaactcgcc gagcgcaccc tggccggggc atttcggacc atcacaccca gccagtgccg
     2041 ggtgatcctg ctcgacgcgg cgcccgcggt gttgccgccg atgggcccga atttgggtct
     2101 caagacgcag cggcggctgg aaaagatgaa tgtcgaggtc cagctcaacg cgatggtgac
     2161 ggccgtcgac tacaacggca tcgcgatcaa ggagaaggac aaaggcgaac gccgcatcga
     2221 atgcgcgtgc aaggtgtggg cggcgggcgt gcaggccagc gcgctgggca agatgatcgc
     2281 cgaacagtcc gacggaaccc aaaccgaccg cgccggacgg gtgatcgtgg agcccgatct
     2341 cagcgtcaag gggcacccgc atgtctttgt cgtcggcgat ctgatgtccg tgcccaacgt
     2401 gccggggatg gcgcagggcg cgattcaagg ggcgaaatac gccacgacgc tgatcaaaca
     2461 gacgctgaaa ggccaagacg accccgccaa ccgcaagccg ttcaagtact tcgacaaggg
     2521 cagcatggcg accatctcgc ggtacagcgc cgtcgcaaag gtcggcaagc tggagttcgc
     2581 cggcttcatc gcctggttgg cgtggctggt gctgcacctg ttctacctgg tcggtcaccg
     2641 gaaccgcatc gccgctttgt tctcctgggt gatgtccttc ttgggtcgca gtcgcggcca
     2701 gatggccatc acgagccaga tgatctacgc cagggtggtg atggaagcgc aaacgctagg
     2761 cggatcgctg gcggtggccg aacgcgccga agaggccgaa cagaaggcgg cgggttagcg
     2821 gcccggcggc agcgacgttc agccgctcgg cggcagcccc ggcggccttg gcggcgcccg
     2881 cggctgcttt atggcactca aagccaaaac gggtagttga acccggtcta tcagctcagg
     2941 gcctggtcga tgtcggcgat cagatcgtcg gtgccctcca gcccgacgga gatgcggacc
     3001 acattgtcac cgagcccgat cgcggcgcgc ccctccggac ccatcgcgcg gtgcgtggtg
     3061 gtcgccggat gagtgatgag cgatttggcg tcgccgagat tgttcgagat gtcgatcagc
     3121 tgcagcttgt ccagtacctc gaaggcccgt tgcttggcag cacttctcgc gcagtcgagc
     3181 gcgaaggtga ccaccgtgcc gccgccggtc atctggcgct tggccaggtc gtattgcggg
     3241 tgcgactcca gaaaggggta gcgcacccaa ctcaccgccg gatggcgctc caggaactcc
     3301 gcaatccggt gtgccgcgga attgctgtgc tcaacccgaa ccgccagcgt ctcgagaccc
     3361 ttcagtagca cccaggcgtt gaaggcactc atcgccgggc cggtgtgccg catcagcttc
     3421 tgcaccggcc cgtcgatgta ttcctggtcg ccgaggatgg ccccgcccag cacccgaccc
     3481 tgaccgtcga tgtgcttggt gcccgaatac accaccacgt cgaccccgag cggaaaacct
     3541 tgctgcagca atggcgtggc gaatacgttg tccagcacga cttttgcgcc cgcagcgtga
     3601 gcgagctcgg tcaccgccgc gatgtccacc agcgactgca tcggattcga cggcgtctcg
     3661 aagaacaccg ccgcggtggg caccgacagc gcctgttccc attgcgcgat gtcgtcgccg
     3721 tcgacgaaga cggtctcgat gccccaccgc ggcaggatct cgttgcacac cacaaaacac
     3781 gacccgaaca ggctgcgcgc cgcgaccaac cggtcaccgg ccgccagcag cgcgcccagc
     3841 gaggtgaaca ccgcggccat gccgctagca gtggcgaacg ccgcgggcgc cccctcgatc
     3901 aggcgcaacc gctcctcgaa catcgtcacg gtcgggttgc cgtaccgcga gtacacgtaa
     3961 tgatccagct cgccgatgaa cgaccgctcc gcgacggcag ccgattcgta gacatacccg
     4021 gaggtcagat acatcgcctc ggcggtctct tcgaaacccg agcgcaacag tccgccgcgc
     4081 acgccgatgg tggcctggct gacgccgtcg ggcagcggct tgggcgtgcg gaccgactcg
     4141 ccgtcggtgt gggtcattgc tgcttccagg gcaacccaac cgcgcgccag ccggtgccgc
     4201 cgcgatgacc gtgggcatcc aggtgaccct cgaaaccgtc cagcacgttg aaggcggggt
     4261 tgatgcccac gtcggtcgcg acctcggccg cgccaatgga gcggttcccg gagcgacaca
     4321 gaaagaccac cggccgctct tcgtcagccg ctgcgggtgg aaccagttcc ttcagctcgt
     4381 cggcgaactg ttcgttgtac atcccgttgg acatattcca ctcgatgtac acgacctcgc
     4441 ggcccagact ggacaggtcg ggcacgccga cgaaccgcca ttcggcctcg gtgcggacgt
     4501 cgaccagcac ggcgcgagga ttgtcggcga gtagcttcca cgcctgaagg ggcgtgatgt
     4561 ctcctgcgta gctcacccgc gtgagtctcg cacattcagg gcgctggtca cctgtcgggc
     4621 gcggtcgcgc gcggtcggca catcgggtgc cgtggccagc gccacgccca gcctggttgt
     4681 cgcgtcgggt gatccggatc gcggcgcgaa aacgcggaga tcagtttccg gcacagccag
     4741 cgcggcggtc agagtggcgg cggtgagcgc cccgccgtcg cggcatgcgt cgaccacatg
     4801 cgctgccccc ggcgacatca tcatggtgtc caacggcagg cccaaaatag cccgggcctg
     4861 cagttcgaat gccgaaagcc gctggctgcg caaggtcacc caggcactct ggtgcaagcg
     4921 ggcggtgacg tcggcgaagt acacctcgtc gccgttgatc atcaattcga caccgaagac
     4981 accccgcccg ccgagcgcct tgacgatccg tgcggcgatc gacttggccg catccaccgc
     5041 ggccgcgctc atctgctgcg gttgccagga ctccaccacg acaccgtcgg cgctgcgata
     5101 cccgatgggt gagcagaact cgattagcgg accctgcggg ccttcgctgc gcaccacgat
     5161 cagggtgacg tcaaactcga tctcgacgat ggtttcggcc attacccgcg gccgctgttc
     5221 gcccgtccca ttcgcgaccg cacgttgcca ggccgcctcg acgtcgtcgg gccccgagac
     5281 caccgagtgt ccccgcccgg acaccggcct caccagcaac ggaaacccgg agtgtgcggc
     5341 caccgccttg agttcggcga ccgatgcgac gaaccagaac ggcgcggtgg gcagcccgag
     5401 ctcgtcggcg gccagccggc gcagaccctc acggtccagg gacagccgcg tgctgcgcgg
     5461 tgtggggaag acctcgatgt cgccgcgctc ggcgacggcg atcagggcg
//
LOCUS       DASSGQ010000048         3782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_125119,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000048 DASSGQ010000000
VERSION     DASSGQ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125119"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="VFI55_02120"
     CDS             complement(<1..1072)
                     /locus_tag="VFI55_02120"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET7072865.1"
                     /translation="MKPTLDDLPLRDDLRGKSPYGAPQLAVPVRLNTNENPHPPTQAL
                     VDDVVRSVGEAAADLHRYPDRDAVALRRDLAGYLSAQTGTPLGVENLWAANGSNEILQ
                     QLLQAFGGPGRSAIGFVPSYSMHPIISDGTRTEWLEAARAEDFGLDVDVAVAAVTDRR
                     PDVVFIASPNNPSGQSVSLPDLRRLLDVVPGILIVDEAYGEFSSQPSAVALVEEYPTR
                     LVVTRTMSKAFAFAGGRLGYLVATPAVIDAMLLVRLPYHLSSVTQAAARAALRHADDT
                     LGSVATLIAERERVSAALAGMGFRVIPSDANFVLFGEFVDAPAAWQRYLDAGVLIRDV
                     GIPGYLRATTGLADENDAFLMAS"
     gene            complement(1069..2415)
                     /gene="hisD"
                     /locus_tag="VFI55_02125"
     CDS             complement(1069..2415)
                     /gene="hisD"
                     /locus_tag="VFI55_02125"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HET7072866.1"
                     /translation="MDNVSATQSSPMARIDLRGTQLTAARLRAALPRGGADVETVLPQ
                     VRSVVQAVAERGAEAALEFGASFDGVRPPTVRVPAAALDAALAGLAPDVSDALQVMIE
                     RTRAVHADQRRADITTTLGPGATVTERWVPVERVGLYVPGGNAVYPSSVVMNVVPAQA
                     AGVGSLVVASPPQARFDGLPHPTILAAARLLGVDEVWAVGGAQAVALLAYGGTDMDGT
                     ELTSVDLITGPGNIYVTAAKRLCRSRVGIDAEAGPTEIAILADDTADPALVAADLISQ
                     AEHDEMAGSVLVTPSEDLAAATDAEVAAQLQTTVHRERVTTALSGSQSAVILVDDLAA
                     GVKVVNAYAAEHLEIQTADATRVAGQIRSAGAIFVGPWSPVSLGDYCAGSNHVLPTAG
                     SARHSSGLSVQTFLRGIHVVDYTEAALKDVSGHVVTLAEAEDLPAHGEAVRRRFER"
     gene            2509..2922
                     /locus_tag="VFI55_02130"
     CDS             2509..2922
                     /locus_tag="VFI55_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET7072867.1"
                     /translation="MSAKDHPNNAPGVPMVFPPWFERLQVKYLNPAFKPIARYTPGTA
                     TIKHRGRTSGKPYETIVTTYRKGDVLAIALAHGKTDWVKNVLAAGGADVQFSPRREVH
                     ITNPRILPAGSDGAGLPFMARVQLRRMGVFVGDIA"
     gene            complement(2941..>3782)
                     /locus_tag="VFI55_02135"
     CDS             complement(2941..>3782)
                     /locus_tag="VFI55_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7072868.1"
                     /translation="EVVAVGDDVGGVQEGDRVVVPFQISCGTCRECGRGVTGSCGSMP
                     LMAMYGMAPIAGLDGGGFMSDLVLVPYADAMLIPVPDTIEPVAIASLSDNIPDGWRAV
                     GPYAAELARLDSADRRVLVVGRLSIGLYAAGIAAALGAHVDYLDTDPQRLAAAEKLGA
                     TVHDRARPDKAWDPYPVTVHTSGDSALLAATLRATWADGVCTDTGIYPEYKVEMPLLP
                     MYTRGVRFVTGRVSARAVIPEVLELLADGLDLSPAVDRVVPWEDAPSAWSTMTGKTVF
                     TRA"
BASE COUNT          617 a         1328 c         1282 g          555 t
ORIGIN      
        1 cgcttgccat gaggaaggcg tcgttctcgt cggccagccc ggtggtggcg cgcaggtacc
       61 cggggatccc cacgtcgcgg atcagcacgc cggcgtccag ataacgctgc cacgcggccg
      121 gcgcgtcgac gaactctccg aacagcacaa agttcgcgtc gctggggatc acccgaaagc
      181 ccatgccggc caacgccgct gaaacccgct cacgttcggc gatcaaggta gcgacgctgc
      241 ccagggtgtc gtcggcgtgc cgcagcgcgg cccgggccgc ggcctgggtg accgacgaca
      301 ggtgatacgg cagccgcacc agcagcatcg cgtcaatcac agcgggggta gcgaccaggt
      361 acccaagcct gccgccggcg aaggcgaacg ccttactcat ggtccgggtg acgacgagcc
      421 gggtcggata ctcctcgacc agcgccaccg cgctgggctg tgacgagaac tcgccgtagg
      481 cctcgtcgac gatcaagatc cccggcacca catcgagcag ccgccgcagg tccggcagtg
      541 aaacactttg tcccgacggg ttgttggggc tggcgatgaa caccacatcg ggccggcgat
      601 cggtgacggc ggcaacggcg acatcgacgt cgaggccgaa gtcctcggcg cgagcggcct
      661 cgagccactc ggtgcgggtg ccatcggaga tgatcgggtg catcgagtag gacggcacga
      721 acccgatggc gctgcgcccg ggcccaccga acgcctgcag cagctgctgc aggatctcgt
      781 tggaaccgtt ggcggcccaa aggttttcga caccgagcgg ggtgcccgtt tgcgcggaga
      841 ggtagccggc cagatcacgg cgcagggcta ccgcatcgcg gtcggggtag cggtgcaggt
      901 cggcggccgc ctcgcccacc gaccgcacca cgtcgtcgac cagcgcctga gtcggcgggt
      961 gcgggttctc gttggtgttc agccgcaccg gtaccgccaa ttgcggtgcc ccatagggcg
     1021 atttgccacg caggtcgtcg cgcagcggca ggtcgtcgag tgtgggcttc atcgttcgaa
     1081 cctccgacgc accgcctcgc cgtgggccgg caggtcttcg gcctcggcga gggttaccac
     1141 gtgtccggag acgtctttga gggccgcctc ggtgtagtcg acgacgtgga tgccgcgcag
     1201 gaacgtctgc accgacaggc cgctggaatg ccgggcagat cccgccgtcg gcagcacgtg
     1261 gttggatccg gcacagtagt cacccaagct taccggcgac cacgggccga cgaaaatggc
     1321 accggccgaa cgtatttggc cggcaactcg cgtcgcgtcg gcggtctgga tctccaggtg
     1381 ttcggcggcg tacgcgttca ccaccttgac cccggcggcc agatcgtcaa ccagaatgac
     1441 cgccgactga ctcccgctca gcgcggtagt cacccgttcg cggtgcaccg tcgtttgcag
     1501 ctgggcggcc acttcggcat cggtggcggc ggccaggtcc tcgctggggg tgaccagcac
     1561 gctgccggcc atctcgtcgt gttcggcctg gctgatcaag tcggcggcca cgagcgccgg
     1621 atccgccgtg tcgtcggcga ggatggcgat ctccgtcggg ccggcttcgg cgtcgatacc
     1681 cacccgcgag cggcacagcc gcttggcggc ggtgacgtag atgttgccgg gcccggtgat
     1741 caggtcgaca gatgtcagct ctgttccgtc catatcggtg ccgccgtagg ccagcaatgc
     1801 caccgcctgc gcaccgccga cggcccacac ctcgtcgact cccagcagcc gggccgcggc
     1861 caggatcgtc ggatgtggca gtccgtcgaa gcgggcctgc ggcgggctgg ccaccaccag
     1921 cgagccgact ccggcggcct gcgccggcac gacgttcatc accacgctgg acgggtagac
     1981 cgcgttgccg ccgggcacat acagaccgac ccgctcgacg ggaacccacc gctcggtgac
     2041 cgtcgcgccc ggcccgagcg tggtggtgat gtcggcgcga cgctgatcgg cgtgcacggc
     2101 gcgggtgcgt tcgatcatca cctgcagcgc gtcggagacg tcgggggcca ggccggccag
     2161 cgccgcgtcc agcgctgcag cgggaacccg gacggtcggt ggccgcacgc cgtcgaagga
     2221 cgccccgaat tccaaggccg cctccgcccc gcgctcggcg acggcctgca cgaccgagcg
     2281 cacctggggc aagaccgtct ccacatccgc gccaccgcgc ggtaacgcgg cccgcaaacg
     2341 ggcggcggtc aactgcgtgc cccgcaggtc gatacgggcc atcggcgacg attgggtggc
     2401 gctcacgttg tccattgtcc cagagcagct caagtcgata tccgaccggt acagtgccgg
     2461 tgtaaggcca cgctcgcgtg tgtgcagagt ttccccaagg gagccgccat gtccgccaag
     2521 gatcacccga acaacgcccc cggcgtcccg atggtgttcc cgccctggtt cgagcgctta
     2581 caggtcaaat acctcaaccc ggcattcaaa ccgatcgcgc gctatacgcc gggaaccgcg
     2641 accatcaagc atcgcggtcg cacgtccggc aaaccctacg agaccatcgt gaccacctac
     2701 cgcaaaggcg atgtgctggc gatcgcgctg gcacatggga agacggactg ggtcaaaaac
     2761 gtgctggccg ccggtggggc cgacgtgcaa ttcagccccc gccgcgaggt gcacatcacc
     2821 aacccacgga tcctgcccgc cggctccgac ggtgctgggc tgccgttcat ggcccgggtg
     2881 cagctgcgcc ggatgggggt cttcgtcggc gacatcgcct gatgctgccg gcgagtgtga
     2941 ctaagccctg gtgaagaccg tcttgccggt catcgtcgac cacgccgacg gggcgtcctc
     3001 ccacggcacg acgcggtcga cggccggcga caggtccaat ccgtcggcca gcagttccag
     3061 tacctccggg atcacggcgc gcgcactgac gcgcccggtg acgaacctca ctccgcgggt
     3121 gtacatcggc agcaacggca tttcgacttt gtattcgggg taaatcccgg tgtcggtaca
     3181 cacgccgtcg gcccaggtgg cgcgcagggt cgccgcgagc agcgccgagt cgccgctggt
     3241 gtgcacagtg accggatacg ggtcccacgc cttgtcgggc ctggcccgat cgtggaccgt
     3301 cgcgccgagc ttctcagcgg ccgccagtcg ctgtgggtcg gtgtccaggt agtcgacgtg
     3361 ggcgcccagc gccgccgcga tccccgccgc gtacagcccg atcgatagcc ggccgacgac
     3421 aagaacgcgg cggtcggccg aatcgagccg cgccagttcc gcggcgtagg gcccgaccgc
     3481 ccgccagccg tcgggaatgt tgtccgaaag ggacgcgatc gcaacgggtt cgatggtgtc
     3541 ggggaccggg atcagcatcg cgtcggcgta gggcaccagc accaggtccg acatgaagcc
     3601 gccaccatcg agcccggcaa tcggtgccat gccgtacatc gccatcagcg gcatcgagcc
     3661 gcatgaaccg gtcacaccgc ggccgcattc gcggcaggtt ccgcagctga tctggaacgg
     3721 caccaccacc cggtcgcctt cctgaacacc gcccacatcg tcaccaaccg cgacgacctc
     3781 gg
//
LOCUS       DASSGQ010000049        17671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_489621,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000049 DASSGQ010000000
VERSION     DASSGQ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 17671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17671
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489621"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VFI55_02140"
     CDS             <1..496
                     /locus_tag="VFI55_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HET7072869.1"
                     /translation="SVAPALRSHCVVAAVRSATVGMPIEASATAPFTDGHWLLSHNGI
                     VDRAVLPPASSAESVCDSAMLAAVIFARGLDALGDTIAEIGAADPNARLNIVAANGSR
                     LVASVWGDTLFMLRRDDGVVLASEPYDDAPDWADVPDRHLVEVTARGVTLTSLAPSGV
                     PKGS"
     gene            496..1461
                     /gene="egtD"
                     /locus_tag="VFI55_02145"
     CDS             496..1461
                     /gene="egtD"
                     /locus_tag="VFI55_02145"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HET7072870.1"
                     /translation="MTLTLSNHLAADSAYHALRRDVFDGLQKTPKSLPPKWFYDSVGS
                     DLFDQITRLPEYYPTRAEAEILRARSTEIASASAADTLVELGAGTSEKTRQLLDALRA
                     RGSLRGFVPFDVDAGMLSATATAIQREYPGVEIEAVCGDFEEHLNEIPGGGRRLFVFL
                     GSTIGNLTPVPRAEFLATLAAVMRPGDSLLLGTDLVKDAGRLVRAYDDAAGVTARFNR
                     NVLAVINRELDADFDVDAYRHVARWNAESERIEMWLRADRRQRVRVGALELSVDFAAD
                     EEMLTEVSCKFRPDGVNAELAGAGLRRIRWWTDSAGDFGLSLAVK"
     gene            1458..2609
                     /gene="egtE"
                     /locus_tag="VFI55_02150"
     CDS             1458..2609
                     /gene="egtE"
                     /locus_tag="VFI55_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis PLP-dependent enzyme
                     EgtE"
                     /protein_id="HET7072871.1"
                     /translation="MTDTDSLADRWRAARPPVAGLHLDSAACSRQSLAVIEATAQHAR
                     NEAEVGGYVAADAATPVLDAGRIAFAALCGMSDADVVFTTGSLHALDLLLGSWPADRP
                     TVACLPGEYGPNLALLAAHGFDRQLLPTLGDGRVALDDAALALDNNPPDFVHLTAVAS
                     HSGVVQPLSMIAKLCGELGLPLVVDAAQALGQVDCAVGADVTYSSSRKWIAGPRGVGL
                     LAVRSGLMEGLRPRLAAPDWAQRSAPLTVARQLEFGEANVAARVGFSVALGEHLAYGP
                     EAVRARLAELGGISRTALADVPGWAVVEEVEEPSAITTLAPVDGADPHAVRSWLLTER
                     RILTTFVGVQRAPLELTAPLLRISPHVDTTADDLATFADALVAATAAVA"
     gene            complement(2691..3381)
                     /locus_tag="VFI55_02155"
                     /pseudo
     CDS             complement(2691..3381)
                     /locus_tag="VFI55_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(3400..4884)
                     /locus_tag="VFI55_02160"
     CDS             complement(3400..4884)
                     /locus_tag="VFI55_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HET7072872.1"
                     /translation="MGEEVKRTTYDRAQRQEYRRKVRRCLDVFETMLSQSRFDSDQPL
                     TGMEIECNLVDADYQPAMSNRFVLDAIADPAYQTELGAYNIEFNVPPRPLPGHTGLDL
                     EAEVRASLNDAEIKANSGGAHIVMIGILPTLMPEHLDDGWMSESTRYAALNDSIFSAR
                     GEDIPINIAGPEPLSWSAASIAPESACTSMQLHLQLAPADFAANWNSAQVLAGPQLAL
                     AANSPYFFGHQLWAETRIEVFAQSTDTRPEELMAQGVRPRVWFGERWIGSILDLFQEN
                     IRYFPSLLPEVSDEDPVAELAAGRAPQLAELRLHNGTVYRWNRPVYDVVDVQGVGRPH
                     LRLENRVLPAGPTVIDMLGNSAFYYGMLRSLSEAEAPLWNSMSFDAAHANFLEAARNG
                     IDARLHWPGLGEVTARELVLDTLLPIAHEGLRRWGVDAEVRDRFLGVIEGRARTGRNG
                     ATWQVSTVRKLEDGGMSRPAALAEMLRRYCEHMHSNEPVHTWNQ"
     gene            5001..6551
                     /gene="glpK"
                     /locus_tag="VFI55_02165"
     CDS             5001..6551
                     /gene="glpK"
                     /locus_tag="VFI55_02165"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET7072873.1"
                     /translation="MEEQQLAKFAESAEYIAAIDQGTTSTRCMIFDHDGAEVARHQLE
                     HEQILPRAGWVEHDPVEIWERTSSVLMSVLNRANLSPKHLLALGITNQRETTLVWNKR
                     TGRPYYNAIVWQDTRTDRIASALDRDGRGDVIRRKAGLPPATYFSGGKLQWILENVDG
                     VREAAESGDALFGTADTWVLWNLTGGPRGGAHVTDVTNASRTMLMDLQTLDWDDELLS
                     FFSIPRAMLPAIAPSSRQQPYGVTLDTGPLGGEVPITGILGDQHAAMVGQVCLDVGEA
                     KNTYGTGNFLLLNTGETIVRSDNGLLTTVCYQFGNAKPVYALEGSIAVTGSAVQWLRD
                     QLGIISGAAQSESLARQVDDNGGVYFVPAFSGLFAPYWRSDARGAIVGLSRFNTNAHL
                     ARATLEAICYQSRDVVDAMRADSGVRLEVLKVDGGITGNDLCMQIQADVLGVDVVRPK
                     VAETTALGAAYAAGLAVGFWADPSDLRANWQEDKRWTPTWSDDKRAAGYAGWRKAVQR
                     TLDWVSVT"
     gene            complement(6582..7298)
                     /locus_tag="VFI55_02170"
     CDS             complement(6582..7298)
                     /locus_tag="VFI55_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET7072874.1"
                     /translation="MSNPFTPPDGPFGGQAGFGFGFGAGPVQHRALHEARRQARRQFH
                     EHLRDHAGGPDFGPGFGPGFGPGFGPGFGPGFGFGPGGRRGGWRRGGGGRGRRGDVRA
                     AILALLAERPMHGYEMIQQIAERSNGIWRPSPGSVYPTLQLLDDEGLITARESDGSKK
                     LYELTDEGRAAAGKVETPPWDEIAEGADPGPVNLRAAIGQLFGAVAQSAHTATAEQQQ
                     RIVEIINNARREVYNILAED"
     gene            complement(7518..8405)
                     /locus_tag="VFI55_02175"
     CDS             complement(7518..8405)
                     /locus_tag="VFI55_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET7072875.1"
                     /translation="MSEVVTGDAVVLDVQIAQLPVRVVSALIDITVIFACYMLGLMLW
                     AATLTQFDSALSAAVLLIFTVLVIVGYPLAFETATRGRSVGKIVMGLRVVSDDGGPER
                     FRQVLFRALASVVEIWMLAGSPAVICSILSPKAKRIGDIFAGTVVVSERGPRSGPPPA
                     MPPSLAWWASSLQLSGLNAGQADVARQFLSRAPQLDRYLRQQMAYRIAGDVVSHIAPP
                     PPPGAPPELVLAAVLAERHRRELARLRLATPVPGPPWGPGSPGGPATAPGTAPWPQQP
                     WPPQQPAPRPHPGGFSPPH"
     gene            8485..9486
                     /locus_tag="VFI55_02180"
     CDS             8485..9486
                     /locus_tag="VFI55_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HET7072876.1"
                     /translation="MDVDAFVLTHRGTWDRLDELVKRRRSLTGAEVDELVELYQRVST
                     HLSILRSARTIGSDSLLIGRLSSLVARARSVVTGAHAPLTRMFTRFWTVSFPVVAYRA
                     WRWWLGTAVVFFAVVVVIALWVAGNPEVQSAIGTPSDIDELVNHDVASYYSEHPAAAF
                     ALQVWVNNSWVAAQCIALSVVLGLPIPFVLFNNAANLGLIAGLMFQAGKGGILLGLLA
                     PHGLLELTAVFLAAASGMRLGWSVISPGDRPRGQVLAEQGRAVVSVAVGLVAVLLVSG
                     LIEALVTPSPLPTFVRVGIGVVAEVAFLSYIVYFGRRAVKAGETGDIDDAPDVIPTR"
     gene            complement(9500..10822)
                     /locus_tag="VFI55_02185"
     CDS             complement(9500..10822)
                     /locus_tag="VFI55_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET7072877.1"
                     /translation="MVLTGRTGLVALICVLPIALSPWPARTFSVLLVLLSILVVVDAG
                     LAASTRGLRYTRSPDSSIRLTQQVDVGVLIHNDGRRRFRGQIRDAWPPSARAEPRIHA
                     VNIAAGQRQHVQTRLRPVRRGDQRAAAVTARSIGPLGLAGRQGSRSVPGQVRVLPPFL
                     SRKHLPSRLAKLQQIDGLLPTLIRGQGTEFDSLREYVVGDDVRSIDWRASARRADVVV
                     RTWRPERDRRVVIVLDTGRTAAGRIGVDPTATDPAGWPRLDWAMDAALLLAALASRAG
                     DHVDFLAHDRISRAAVFGASRTELLAQLVEAMAPLQPALIESDWRAMVAAVARRTRRR
                     SLVVLLTDLNATALDEGLLPVLPQLSARHHMLIAAVADPRVDQMAAGRSDAAAVYDAA
                     AAERSRNDRRAIAARLRRSGVEVVDAAPTELAPALADRYLAMKATGRL"
     gene            complement(10822..11820)
                     /locus_tag="VFI55_02190"
     CDS             complement(10822..11820)
                     /locus_tag="VFI55_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET7072878.1"
                     /translation="MTQPSPSTAAESPTSEPTSASAREALLALRAELAKAVVGQEGVV
                     SGLVIALLCRGHVLLEGVPGVAKTLLVRALSAALQLEFKRVQFTPDLMPGDITGSLVY
                     DARTAAFVFRPGPVFTNLLLADEINRTPPKTQAALLEAMEERQVSVDGEPKPLPDPFI
                     VAATQNPIEYEGTYQLPEAQLDRFLLKLNVTLPSRDSEIAILGRHAHGFDPRDLSAIK
                     PVAGPAELAAGRQAVQQVLVADEVLGYIVDIVGATRSSPALQLGVSPRGATALLGTAR
                     SWAWLSGRNYVTPDDVKAMARPTLRHRVMLRPEAELEGATPDGVLDGILASVPVPR"
     gene            complement(11817..12968)
                     /locus_tag="VFI55_02195"
     CDS             complement(11817..12968)
                     /locus_tag="VFI55_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HET7072879.1"
                     /translation="MATTAAHPDAVTTTVARRRRSWRGVVLTLIALAAIAVIGAYLTA
                     PRHGARMDPESTDPDGAHALVTLLRDFGVEVVVAHGIADVERAARPGSLILVAQTQYL
                     TDATLLDRLAKTPGDLLLMEPTVRTRDALMPDVRMSTASTFDSNPNCALREATRAGMV
                     RFGPSDTYQATDGRTLTRCYDGVLIRFRENGRTVTAVGSTDFMTNDGLLQAGNAALAM
                     NLAGDRPRLIWYAPQRAEGETSTSTSLVDLIPENAIWIVVQLCLVVLLVALWKGRRPG
                     PLVAEELPVVVRASETVEGRGRLYRSRRARDRAAAALRTATMQRLLPRLGLGPDAPAP
                     AVVTTVAQRTGSDPGFVAYQLFGPPPATDNDLLQLARALDEIERQVTRT"
     gene            complement(12968..13621)
                     /locus_tag="VFI55_02200"
     CDS             complement(12968..13621)
                     /locus_tag="VFI55_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HET7072880.1"
                     /translation="MPSIDIDSDAAHQAAQVELDKPIYSKGSVAQQFAEWVNELLYRL
                     LQHASSLPGGWLTSSVLLILLVIAVVVAIHVARRTMRTRRRGDHPLFDAGQLTAAQHR
                     ATAEGYAAQGDWAAAIRHRLRAVARQLEETGVLEAAPGRTANELASDAGTALPHLAGE
                     LSQAAMAFNDVTYGEQPGTQASYQMIVDLDDHLRSRSAAVGTAAGQPAVIESWAQVR"
     gene            complement(13646..14980)
                     /locus_tag="VFI55_02205"
     CDS             complement(13646..14980)
                     /locus_tag="VFI55_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072881.1"
                     /translation="MPETLRDGTPVKIEKCPDRRQDGAHDERRARLRCLFQSWFARPL
                     HTVNAPPPGYPLGPPGGYVPAGYPPPAYGPPPGYGPPPGYGPPLGYGPPPGYGAPPGY
                     GPPPGYGPPPGYGPSPVVPGALKPGIIPLRPLTLSEIFNGAVGYIRANPKATLGLTAM
                     VVVAMQIISLLATVGPLAAYGGINTERSDQLSPGVVGAWMASMGASVLVTWLAGMLLS
                     GMLTVIVGRAVFGSSITISETWAKIRGRLLPLLVLALLEAVVLAAVFGLVAVAIALLA
                     AVGNPGAAVVLGLPLVLVVIALIMYLYTVVLFAPVAIVLERLPVIDAISRSFALVRNG
                     FWRVLGIRALTFVVASVVAYAVAIPFSIVGQVILLGTPSTATLLLGTAIASVGSAIGQ
                     IITAPFSAGVVVLLYTDRRMRAEAFDLVLQSGAAGGPYAVASTDNLWLTRPV"
     gene            14979..15443
                     /locus_tag="VFI55_02210"
     CDS             14979..15443
                     /locus_tag="VFI55_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET7072882.1"
                     /translation="MAELKSRLRADLTKAMKNQDKLRTATLRMVLAAIQTEEVSGKQA
                     RELSDDEVIKVLARESRKRAEAAEIYTQNGRGELAAEEHAEARVIDEYLPTQLTEAEV
                     ADVADTAIAQVAEELGERPGMKQMGQVMKAATAIAAGKADGARLSAAVKERL"
     gene            15524..>17671
                     /locus_tag="VFI55_02215"
     CDS             15524..>17671
                     /locus_tag="VFI55_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HET7072883.1"
                     /translation="MLIRVRSPRQILELLRSKDPERDALRRAIRAAIMVPFTVGFAFV
                     VVGGKATPLYALLGAFWLLVVTEFPGNRQQRAVAYLGLALNGFVLITLGTLVAPIAWL
                     AVTLMFLLGVAVTLAGMLSATMSAGQRATLLFYVWPVCTPVGPIGERLLGWLIALVLC
                     VPAALFFLPPRHYDDLRRHAARVCAALADRIEGVCSTMDVTAAMDALRSNFLAANFRP
                     VGLSAGSRALVRIVDFLELVADLVDDDAGTTLGPVKPPAVAVLRCCSGLLDASQLAQR
                     ATNRAGLEQELPRLRSLARGRYRDDVLLMLSAPDDAGAVEVGERLLACRVIATTIGLT
                     GRVIAAAATADARPVWARALGLQLPPTGFADRLSPEAVAGTTIVTAFLASRSVAAHNG
                     LRIGVGLALAVALTHLHLVERGFWVVVGTMLVISSSALATRTKVVQAVVGTALGIIFG
                     GLLVAAVGVAPVAQWLLVPITVFLATYLPRFASFTIAQAMGALNLLTILSLTIPSGWH
                     LGLLRIEDIAMGAAVGMGVSLLLWPRGATAVVSKLIGAAREVNVRYLQAAVLRVTRDH
                     PEEGPEETDAALAELSHHAVVGSRRVDDAVRHYLSETGSGADLRTPVVRAANRATWVR
                     LTADMIADIRTLPPTGAYPSARALLEAHLEFATNQVFGRVEEINRPIAGEFARALRVE
                     ATNQGEAVEAAQALVTVAANLAQLEMVTAVESET"
BASE COUNT         2735 a         5811 c         6232 g         2893 t
ORIGIN      
        1 atcggtcgcg ccggcgctgc gtagccactg cgtggtggcc gcggtgcgct cggcgaccgt
       61 cgggatgccg atcgaggcca gcgcgaccgc gcccttcacc gacggccact ggctgttgtc
      121 gcacaacggc atcgtcgatc gcgcggtgct gccgcccgcg tcgtcggcgg aatcggtgtg
      181 cgacagcgcg atgctggccg ccgtgatctt cgcgcgtgga ctcgacgcgc tgggcgacac
      241 catcgccgag atcggggcgg ctgacccgaa cgcacgactg aatatcgtgg ccgccaacgg
      301 ttctcgtctt gtggccagcg tgtggggcga cacgttgttc atgctgcgcc gtgacgacgg
      361 tgtggtgctg gccagcgagc cgtatgacga tgcgcccgac tgggcggacg tgccggaccg
      421 gcacctggtt gaggtcaccg cgcgcggtgt caccctgact tccctggccc cctctggagt
      481 cccgaaagga tcttgatgac gctgacgctg tccaaccacc tcgccgccga ctctgcgtat
      541 cacgcgttgc gtcgcgatgt gttcgacggc ctgcagaaga caccgaagtc gttgccgccc
      601 aagtggtttt atgattcggt gggcagcgat ctgttcgacc agatcacccg gttgcccgag
      661 tactacccga cccgcgccga ggccgagatc ctgcgtgccc gatcgaccga aatcgcgtcg
      721 gcgagcgcag cagacacgct ggtcgagctg ggcgccggaa cgtcggagaa gacccggcag
      781 ctgctggacg cgctgcgtgc ccgcgggtcg ctacgcggat tcgtcccgtt cgacgtcgac
      841 gccggcatgc tctccgcgac ggcgaccgcg atccagcgcg aatacccggg cgtcgaaatc
      901 gaggcggtct gcggggattt cgaggagcac ctcaacgaga ttcccggcgg gggccggcgg
      961 ttgttcgttt tcctgggctc gacgatcggc aatctgacgc ccgtcccgcg cgcggagttt
     1021 ctcgcgaccc tggccgcggt gatgcggccg ggggacagcc tgctgctggg caccgacctg
     1081 gtcaaggacg ccgggcggct ggtgcgcgcc tatgacgacg ccgccggcgt gacggcccgg
     1141 ttcaatcgca acgtgctcgc cgtgatcaac cgagaactcg acgccgattt cgacgtcgac
     1201 gcctaccggc acgtggcccg ttggaacgcc gaatccgaac gcatcgagat gtggctgcgc
     1261 gccgaccgtc gccagcgggt gcgggtcggc gcgctcgaac tgagcgtgga tttcgcggcc
     1321 gacgaggaaa tgctgaccga agtgtcgtgc aagttccgtc ccgacggggt gaacgccgaa
     1381 ctggccggcg ccggactgcg ccgcattcgg tggtggaccg atagtgcggg tgacttcggt
     1441 ctttcgctgg ccgtgaagtg accgataccg attcgctggc cgaccgatgg cgggcggccc
     1501 gtcccccggt ggccgggctg cacctggaca gcgcggcctg ctcccgccag agcctcgcgg
     1561 tgatcgaggc caccgcccag cacgcacgca acgaggccga agtcggcggg tacgtcgcgg
     1621 ccgacgcggc cacacccgtg ctggacgccg ggcggatcgc gttcgccgcg ctgtgcggca
     1681 tgtccgacgc cgacgtggtg ttcaccacgg gctcactgca cgcgttggac ctgttgctgg
     1741 gcagctggcc ggcggatcgg ccaacggtgg cctgcctgcc cggcgaatac ggtcccaacc
     1801 tggccctgct ggccgcccac ggatttgacc gacagctgct cccgaccctg ggtgatggcc
     1861 gggtcgcgct cgacgacgcc gcgctggcgc tggacaacaa cccgcccgat ttcgtgcacc
     1921 tgaccgcggt ggccagtcat agcggtgtgg tgcaaccgct gtcgatgatc gccaagctct
     1981 gcggcgagtt ggggctgccg ctggtcgtgg acgccgccca ggcgctgggc caggtggact
     2041 gcgcggtggg cgccgacgtg acctactcct cgtcgcgtaa gtggattgcc gggccccgcg
     2101 gggtgggcct cctggcggtg cgctccggtc tgatggaggg tttgcgcccg cggctggccg
     2161 cgccggactg ggcccagcgg tcggcgccgc tgacggtggc ccggcaactc gaattcggcg
     2221 aggccaatgt cgccgcgcgc gtgggctttt cggtggccct cggggaacat ctggcatacg
     2281 gacccgaggc tgtgcgcgca cgtctggccg aactgggcgg catcagccgg acggcgctgg
     2341 ccgacgtgcc cggctgggca gtggtcgaag aggtcgagga gccgagtgcg atcaccaccc
     2401 tggccccggt cgacggtgcc gatccgcacg cggtgcgtag ctggctgctc accgaacgcc
     2461 ggatcctgac cacgttcgtc ggggtgcagc gggcaccgct ggagctcacg gcgccgctct
     2521 tgcggatctc gccgcacgtg gacaccacgg ctgacgacct ggccaccttc gccgacgcgc
     2581 tggtggccgc gaccgccgct gtggcgtaac gacgcttagt tcgccgaaat cgacacccgc
     2641 gcgggaaagt tcgagaaaga gcgcggtagc gtcgatttcg acgcagcagg tcaggtcttg
     2701 tgggcggtca gcaggtaggc cggtaacttc atccggtcct tttcgtcgcg gtcgtggggc
     2761 gggaattcga acggcgcctc gggggggccg ccctgcggga tgttcgcatg aatgtagccg
     2821 ggccggattt cgtcgatctc ccagtacttg ctcaccgcgg cgcgcaactc atcgtcgtcc
     2881 accgggttgg gacccgtctc catctcggcg gggaaggcgc ccttggcgaa caccagcacg
     2941 aagtagctgg cccctggggc cgccgcgcgg tggatggcgc tcaggtaccc gtcgcgagcc
     3001 tcgatgggta gcgaatgaaa cagcgtgctg tcgacgatgg tgctgaaccg gccttctgat
     3061 cccggtgggt agctcgtgaa atcggtgatg tcggccttcg cgaagctggc ggcggccagg
     3121 ccgcggtcgg cagccgcttt ggtggccgcc gcgaccgcgg tcggcgtgag atcgatgccg
     3181 acgacggtgt aaccctgcgc ggccagcccc aaggacagtt cggcgacccc gcaaccggcg
     3241 tcgagcacat cgctgcggaa ctggccgttc gcgatcagcg cggccagttc gggctgcggt
     3301 tcaccgatgt tccacggtgg tggaccctcg aattctccct gctcggtaag cgctgtccca
     3361 gtcgatcagt cctggtgcca tggttccccc taggcgctgc tattgattcc aggtatgcac
     3421 cggttcgttg ctgtgcatgt gttcgcagta ccgccgtagc atctctgcca gtgccgccgg
     3481 ccggctcata ccgccgtctt ccagcttacg cacggtggac acctgccagg tggctccgtt
     3541 gcgcccggtc ctggcgcggc cctcgatgac acccaggaac cggtcgcgca cctcggcgtc
     3601 gacgccccac cggcgcaggc cttcgtgggc aatcggcagc agcgtgtcga gcaccagttc
     3661 ccgcgccgtc acttcgccca ggccgggcca gtgcagccgg gcatcgatgc cgttgcgggc
     3721 cgcttcaagg aaattcgcgt gtgccgcatc gaaactcatg ctgttccaca gcggcgcttc
     3781 cgcttcggac aagctgcgca gcatgccgta gtagaaggcc gaattcccca gcatgtcgat
     3841 gaccgtcggg ccggccggca atacccggtt ttccagccgc agatgcggac gcccgacgcc
     3901 ctgcacgtcc acgacgtcgt acaccggccg gttccaccgg tacacggtgc cattgtgcag
     3961 gcgcagttcg gcgagttgcg gggcgcgtcc ggcggccagt tcggcgaccg ggtcctcgtc
     4021 ggaaacctcg ggcaacaggg aggggaagta gcggatgttc tcttgaaaca ggtccaggat
     4081 ggagccgatc catcgttcgc cgaaccacac ccgcggtcgc acgccctggg ccatgagctc
     4141 ctcgggccgg gtgtcggtgg actgcgcgaa cacctcgatc cgggtctccg cccacagctg
     4201 gtggccgaaa aagtagggcg agttagcggc cagcgccagc tgcggaccgg cgagcacctg
     4261 agccgaattc cagttggcgg cgaaatcggc cggcgccagc tgcaagtgca attgcatgct
     4321 ggtgcaggcg gattcgggcg cgatggacgc ggcgctccaa ctcagcggct ccgggccggc
     4381 gatgttgatc gggatgtctt cgccgcgggc ggaaaagatc gagtcgttga gggccgcata
     4441 ccgcgtcgac tcgctcatcc agccgtcgtc gagatgttcg ggcatcagcg tgggcaggat
     4501 cccgatcatc acgatatggg cgccgccgga gttggccttg atctcggcat cattgaggct
     4561 ggcgcgcact tctgcctcga ggtccagacc cgtgtggcca ggcagcggac ggggtggcac
     4621 gttgaattcg atgttgtagg cgcccaattc ggtctggtag gccgggtctg cgatggcatc
     4681 cagcacgaac cggttcgaca tggccggctg atagtcggcg tcgacgaggt tgcactcgat
     4741 ctccatgccg gtgagcggct ggtcggaatc gaagcgcgac tgcgagagca tcgtctcgaa
     4801 gacgtccaaa caccgccgca ccttgcgccg gtactcctgc cgttgtgcgc ggtcgtacgt
     4861 ggtgcgcttg acctcttcgc ccacaacgcc gatgcaacag gtttgccggc ggtaacgcaa
     4921 gggcgacggg ctgacctgcg ctgtcgtctg gtggagtggg tagaccatca tggaccctga
     4981 ccgcaagtgc cacaccacgg gtggaggagc agcagttggc taagttcgcg gaatccgctg
     5041 agtacatcgc cgccatcgat cagggcacca ccagcacccg ctgcatgatt ttcgatcacg
     5101 acggcgccga agtggctcgc catcagctcg agcacgagca gatcctgccc cgcgccggct
     5161 gggtcgagca cgaccccgtc gaaatctggg agcgcacctc gtcggtgctg atgtcggtgc
     5221 tgaaccgggc caacctttcg ccgaaacacc ttttggcgct ggggattacg aatcagcgcg
     5281 aaaccacgct ggtatggaat aagcggaccg gcagaccgta ctacaacgcg atcgtctggc
     5341 aggacacccg caccgaccgg atcgcttcgg cgctggaccg ggatggccgt ggcgacgtga
     5401 tccgccgcaa ggcgggactg ccgccggcga cctatttctc cggcggaaag ctgcaatgga
     5461 tcctggagaa cgtcgatggg gtgcgcgagg ccgcggaaag cggtgacgcc ctcttcggca
     5521 ccgccgacac ctgggtgttg tggaatctga ccggtggtcc gcggggcggc gcgcacgtca
     5581 cggacgtgac caacgccagc cggaccatgc tgatggatct tcagaccctg gactgggacg
     5641 acgagctgtt gtcgttcttc tccatcccgc gcgcgatgct gccggcgatc gcgccgtcgt
     5701 cgcggcaaca gccctacggt gtgacgctgg acaccgggcc cctcggcggc gaagtgccga
     5761 tcaccgggat cctcggcgac cagcacgcgg cgatggtggg ccaggtgtgc ctggacgtgg
     5821 gcgaggcgaa aaacacgtac ggcactggca attttcttct gctcaatacg ggcgagacga
     5881 tcgtgcgatc ggacaacggc ctactgacca ccgtctgtta tcaattcgga aacgccaaac
     5941 ccgtttacgc gcttgagggt tcgatcgcgg tcaccggttc ggcggtgcag tggctgcgcg
     6001 atcagctggg catcatcagc ggtgccgcgc aaagcgagtc gctggcccgt caggtcgacg
     6061 acaacggcgg ggtgtatttc gtgccggcgt tttccgggtt gttcgccccg tattggcgat
     6121 ccgacgcccg tggcgcgatc gtggggctgt cgcggttcaa caccaacgcg cacctggctc
     6181 gggcgacgct ggaggcgatt tgctatcaga gccgcgacgt ggtcgacgcg atgagagcgg
     6241 attccggtgt gcgccttgag gtattgaagg ttgacggcgg catcaccggc aatgacttgt
     6301 gcatgcagat ccaggccgac gtgctgggcg tggacgtggt gcggccgaag gtcgccgaga
     6361 ccaccgcgct gggcgccgcc tacgccgcgg gtttggcggt cgggttctgg gccgatccgt
     6421 ccgacctgcg cgccaactgg caagaagaca agcgctggac gccgacgtgg agcgacgaca
     6481 agcgcgccgc cggatatgcg ggttggcgca aggccgtgca gcggaccctg gactgggtct
     6541 cggtaacgtg atccgtccta atccagggtc cgcggcaggg atcagtcctc ggcgaggatg
     6601 ttgtagacct cgcgtcgggc attgttgatg atctcgacga tgcgctgctg ctgttcggcg
     6661 gtggcggtgt gcgcggattg cgcgaccgcg ccgaacaact ggccgatggc cgcccgcagg
     6721 ttcacggggc cggggtcggc gccttcggcg atctcgtccc acggtggggt ctcgaccttg
     6781 ccggctgccg cgcggccctc gtcggtcagt tcgtagagct tcttactgcc gtcggattcc
     6841 cttgcagtaa tcaggccttc gtcgtcgagt agctgcagcg tcgggtacac cgacccgggg
     6901 ctgggccgcc agattccgtt gctgcgctcg gcgatctgct ggatcatttc gtagccatgc
     6961 atgggccgct cggccagcag cgcgaggatg gctgcccgca cgtcaccgcg tcgtccgcgg
     7021 ccaccgccgc cgcgacgcca tccgccgcgt cggccacccg ggccgaagcc gaatcccggg
     7081 ccgaatcccg gcccgaagcc tggaccgaag cccggaccga acccgggacc gaaatccggg
     7141 ccgccggcgt gatcgcggag gtgttcgtgg aattgccgtc gggcctgtcg cctggcctcg
     7201 tgcagggcgc ggtgctgaac cggcccggcg ccgaacccga agccgaaacc tgcctggccg
     7261 ccgaagggcc cgtcaggggg agtgaatggg ttgctcattg gttgtggtcc tctcaaaggt
     7321 ttgtggtccc catgtctggg gccgcgcact cgtcgtgcgc ttcggtatat cacgatatat
     7381 cgtaaactaa cgtgatgcaa cgagatattc ccacattcgg gcaaaaccgg gtgtcccggt
     7441 ggcgtgacct gcgggaaagc ggaactatgc gcgtcgcaag cccggcgcgg cggcgcggtc
     7501 taaagccggc gctgcagtta gtgaggcggc gagaagccgc ccgggtgcgg ccgaggggcg
     7561 ggctgctgcg ggggccatgg ctgttggggc cacggcgcgg tgccgggtgc cgtggccggc
     7621 ccgcccgggg acccaggtcc ccacggtggg cccgggacgg gtgtggcaag ccgaagccgc
     7681 gccagttcgc ggcggtgccg ttcggccagc acggccgcaa gcaccaactc cggaggggcg
     7741 cccggtggtg gcggcggcgc gatgtgcgac actacgtcac cggcaattcg gtatgccatc
     7801 tgctgccgta gataacgatc gagttgtggt gcacgggaga ggaattggcg tgcgacgtcg
     7861 gcttggccgg cgttcagtcc cgacagttgc agcgacgaag cccaccacgc caacgaaggc
     7921 ggcatcgccg gcggcggccc cgatcggggg ccgcgttcgc tgaccacgac ggtgccggcg
     7981 aagatatcgc cgatgcgctt tgccttcggc gacaagatgc tgcagatgac ggcggggctt
     8041 cccgccaaca tccagatctc caccaccgac gccagcgcgc gaaacagcac ctgccggaag
     8101 cgttctggac cgccgtcgtc ggacaccacg cgcaggccca tcacgatctt gcccaccgac
     8161 cggccacggg tcgcggtttc aaatgccaag ggatacccga caatcaccag caccgtgaaa
     8221 atgagcagga cggcggcgct gagcgcactg tcgaactggg tcagggtggc cgcccacaac
     8281 atgagcccca gcatgtagca ggcaaaaatc acggtgatgt cgatcagcgc gctcaccacc
     8341 cgcaccggca actgggcgat ctggacgtcg agcaccacgg cgtccccggt caccacctcc
     8401 gacataacac cgaacgtaca ggtttcgcgg gtggcaccga ccgggcgtgc ccccagcgcc
     8461 gaatccccga ttaggctgcg cagggtggat gtcgacgcat tcgtgctgac ccatcgaggc
     8521 acgtgggacc ggctcgacga gctggtcaag agacgccggt cactgaccgg cgccgaagtc
     8581 gacgagcttg tcgagctcta ccagcgggtt tcgacccatc tgtcgatact gcggtcggcc
     8641 aggacgattg ggtcggattc gctgctgatc ggccggcttt cgagtctggt ggcgcgcgcc
     8701 cgttcggtgg tgaccggcgc ccacgcgccg ctgaccagga tgttcacccg attctggacg
     8761 gtgtctttcc cagtggttgc ataccgcgcc tggcgctggt ggctgggcac ggccgtggtg
     8821 tttttcgccg tcgtggtggt catcgcgctg tgggtggccg gcaatccgga ggtgcagtcg
     8881 gccatcggaa cgccaagcga catagacgaa ttggtcaacc acgatgtcgc gtcgtactac
     8941 agcgaacacc ccgcggcggc gttcgcctta caagtttggg tgaataactc ctgggtcgcc
     9001 gcgcagtgca tcgcgttgtc cgtggtgctg gggctgccga ttccgttcgt gctcttcaac
     9061 aacgccgcca acctcgggct gatcgccggg ctgatgttcc aggccggcaa gggcggcatc
     9121 ctgctgggcc tgctggctcc gcacgggttg ctggagctga cagccgtgtt cctcgcggcg
     9181 gcgtcgggga tgcggctggg gtggtcggtg atatcgcctg gggatcggcc gcgcggacaa
     9241 gtgctcgccg aacagggccg cgccgtcgta tcggtggccg tggggctggt ggctgtgctg
     9301 ttggtgtccg ggttgatcga agcactggtg acgccgtcgc cgttgccgac gttcgtccgg
     9361 gtgggcatcg gcgtggttgc cgaagtggcg ttcctgtcct acatcgtgta cttcggtcgt
     9421 cgcgcggtga aagccggtga gaccggcgat atcgacgacg cacccgatgt gatccccacc
     9481 cgctgagcgc caccgaaagt catagtcgcc cggtggcttt catcgccaga tagcggtcgg
     9541 ctagggcggg agccagttcc gtgggggcgg cgtcgacgac ttccacgccg ctgcggcgca
     9601 gccgcgccgc gatcgcgcgg cggtcgttgc gggatcgttc ggcggccgcc gcgtcgtaca
     9661 cggcggccgc atccgaacgg cccgcagcca tttggtcgac gcggggatcg gcgaccgcgg
     9721 cgatcagcat gtggtgtctg gccgacaact gcggcagcac cggcagcagg ccctcgtcca
     9781 aagcggtggc gttgaggtcg gtcagcagca ccaccagcga ccgccgcctg gtgcgccgcg
     9841 cgacggcggc aaccatggcg cgccaatcgg attcgatcag tgccggttgc aggggagcca
     9901 ttgcctcgac gagctgggcg agcagttcgg tgcgcgacgc gccgaacacg gcggcccggc
     9961 tgatccggtc gtgtgcgagg aagtcgacat ggtcgccggc ccgcgacgcg agcgcggcca
    10021 gcagcagcgc ggcgtccatc gcccagtcca gccgcggcca gcccgcggga tccgtggcgg
    10081 tggggtcaac gccgatgcgg cccgctgcgg tgcggccggt gtcgagcacg atcaccacac
    10141 gccggtcgcg ttcgggtcgc caggtgcgga cgacgacgtc ggcgcggcgc gcgcttgcgc
    10201 gccagtcgat cgagcgcacg tcgtcgccga ccacatactc acgcaacgaa tcgaattcgg
    10261 ttccctgccc gcggatcagg gtgggcagca gtccgtcgat ctgctgcagc ttggccagcc
    10321 gtgacggcag gtgcttgcgc gacagaaacg gcggcagcac ccggacctgg cccggcaccg
    10381 accgcgagcc ctgccgtccc gccagcccca gcggcccgat cgaccgggcg gtgaccgcgg
    10441 ccgcccgctg gtcgccccgg cggaccggcc gcagccgggt ctgcacgtgc tgacgttggc
    10501 ccgcggcgat gttgacggcg tgtatgcgcg gctcggcgcg ggcgctgggc ggccacgcgt
    10561 cgcggatctg gcctcgaaac ctgcgtcggc cgtcgttgtg gatcagcacg cccacgtcca
    10621 cctgctgggt cagccggata gagctgtccg gtgaacgcgt ataacgcaat ccgcgggtgc
    10681 tggccgccag cccggcatcg acgaccacca gtatcgacag cagcactagc aacaccgaga
    10741 aagtccttgc cggccaagga gacagcgcga tgggcaggac gcagatcagc gcgaccagcc
    10801 cggtgcgtcc ggtcaggacc actagcgggg caccggaacc gaggccagga ttccgtcgag
    10861 cacgccgtca ggcgtggctc cttcgagctc ggcctccggg cgcagcatca cccggtgcct
    10921 cagcgtcggg cgggccatgg ccttcacgtc atcgggagtg acgtagttgc ggccggacag
    10981 ccacgcccag gaccgggccg tgcccagcag tgccgttgcc ccgcgcggcg agacacccag
    11041 ttgtaacgcg ggagaggagc gggtggcccc gacgatgtcg acgatgtagc ccagcacctc
    11101 gtcggcgacg aggacttgct gcaccgcttg ccggccggcc gccagctcgg cggggccggc
    11161 caccggcttg atcgcggaca gatcgcgggg atcgaagccg tgcgcgtgcc tgcccagaat
    11221 ggcgatctcg gagtcgcgcg acggcagtgt gacattcagt ttcagcagga agcggtccag
    11281 ttgggcttcg ggcagctggt aggtgccctc gtattcgatc gggttctgcg tcgcggcgac
    11341 gatgaaggga tcgggcaacg gcttgggttc gccgtccacg ctgacctgac gctcctccat
    11401 cgcctcgagc agcgcggcct gggttttggg tggtgtgcgg ttgatctcgt cggcaagcag
    11461 caggttggtg aacaccgggc ccggccggaa cacgaatgcg gcggtgcgcg cgtcgtacac
    11521 cagcgaaccg gtgatgtcgc cgggcatcag atcgggggtg aactggaccc gcttgaattc
    11581 cagttgcagc gcggcggaca gcgcccggac cagcagcgtc ttcgccactc ccggaacacc
    11641 ttccagcagc acgtggccgc gacacagcag cgcgatcacc aggccgctga ccactccctc
    11701 ctgtccgacg acggccttgg cgagttcggc gcgcaacgcc agcagcgctt cgcgtgccga
    11761 tgccgaggtc ggttccgagg tggggctctc ggctgcggtg gaaggggacg gttgcgtcac
    11821 gtgcgtgtga cctgcctttc gatttcgtcg agggcgcgag caagttgtag gaggtcgttg
    11881 tcggtggccg gcggcggccc gaacagttgg taggcaacga accccgggtc ggatccggtg
    11941 cgctgggcca ccgtcgtcac caccgcgggc gccggtgcgt ccgggcccag gccgagccgg
    12001 ggcagcaggc gctgcatcgt ggctgtgcgc agtgccgctg cggcgcgatc gcgcgcccga
    12061 cgggagcggt agagccgacc gcgtccctcg acggtctccg aggcacgcac cacaacgggc
    12121 aactcctcgg ccaccagcgg tccgggccgg cgtcccttcc acagggcgac cagcagcacg
    12181 accaggcaca gctgcaccac aatccagatg gcgttctccg ggatcaggtc gacaagcgat
    12241 gtggaagtgg aggtttcgcc ctcggcgcgc tgcggtgcat accagatgag gcggggtcgg
    12301 tcgcccgcaa ggttcatcgc cagcgcggcg ttgcccgcct gcaacaggcc atcgttggtc
    12361 atgaaatcgg tgctgccgac cgcagtgacg gtccgcccgt tctcccggaa tcggataagt
    12421 accccgtcgt aacagcgggt caatgtccgg ccgtcggtgg cctggtaggt gtcgcttggc
    12481 ccgaatcgaa ccattccggc tcgagtcgcc tctcgcagag cgcaattcgg gttgctgtcg
    12541 aaggtgctgg cggtcgacat gcgcacatcc ggcatgaggg cgtcgcgggt tcgcaccgtc
    12601 ggttccatca ggagcaggtc gccgggggtt ttcgccagcc ggtccagcag cgtggcgtcg
    12661 gtgaggtact gggtctgcgc gaccaggatc agcgagccgg gccgcgctgc ccgttcgaca
    12721 tcggcgatgc cgtgggcgac gacgacctcg acgccgaagt cgcgcagcag cgtcaccaat
    12781 gcgtgggcgc cgtcgggatc ggtcgattcc ggatccatcc gggcaccgtg ccggggtgcg
    12841 gtcagatacg cgccgatcac cgcgatggcc gcgagcgcaa tcagcgtgag aacgacccca
    12901 cgccatgaac gccgtcgccg cgccaccgtc gtggtcacgg cgtccggatg cgccgcggtg
    12961 gtcgccatca ccgcacctgc gcccaggatt cgatcacggc gggctggccc gccgccgtgc
    13021 cgacggccgc cgatcgcgac cgcaggtggt cgtcgaggtc gacgatcatc tggtaggagg
    13081 cctgggtccc gggctgctcg ccgtaggtca cgtcgttgaa tgccattgct gcctgcgaca
    13141 attcgcctgc cagatgtggt aacgccgtac ccgcgtcgga ggccagctcg ttggcggtgc
    13201 ggcccggtgc cgcttccagg acgccggtct cctcgagctg acgggcgacc gcgcgcagtc
    13261 ggtgccggat cgctgcggcc caatcacctt gggccgcata gccttcagcg gtggcccggt
    13321 gctgtgccgc ggtgagctgg ccggcgtcga acaacgggtg gtcgccgcgg cggcgggtgc
    13381 gcatggtgcg ccgtgcgaca tggatggcga ccaccaccgc gatcaccagc aggatgagga
    13441 gcaccgagct ggtcaaccac ccgcccggca gcgaggaggc gtgctgcagc agccggtaca
    13501 gcagttcgtt gacccactcg gcgaactgtt gtgccacaga gcctttggaa tagatcggct
    13561 tgtccagttc gacctgtgcg gcctgatgtg cggcatcgct gtcgatgtcg atggaaggca
    13621 ctgtcagctg atccttaatc gggccctaca cgggccgcgt cagccagagg ttgtcggtgg
    13681 atgcgacggc gtagggtccg ccggccgccc cgctctgcag caccaggtcg aacgcctcgg
    13741 cgcgcatgcg ccggtcggtg tagagcagca cgacgacgcc cgcactgaat ggcgcggtga
    13801 tgatctgtcc gatcgccgac ccgactgatg cgatcgcggt gcccaacagc agagttgcgg
    13861 tggacggcgt cccgagcagg atgacctggc cgacgatgct gaacggtatc gcgacggcgt
    13921 aggcgaccac ggatgccacc acgaacgtca gcgcccggat ccccaatacc cgccagaagc
    13981 cgttgcgcac cagggcgaag gatctgctga tcgcatcgat gacgggcagc ctctccagca
    14041 cgatcgccac gggcgcgaac aacaccaccg tgtagaggta catgatcaac gcgattacga
    14101 ccagcaccag cggcaggccc aagacaaccg ccgccccggg attgccgacc gccgccaaca
    14161 gcgcgatcgc gacggccacc agcccgaaca ccgcggccag cacgacggct tccagcagcg
    14221 ccagcacgag caatgggagc agccgccccc ggatcttggc ccaggtttcg ctgatcgtga
    14281 tcgacgaccc gaacaccgcc cgcccgacga tgacggtgag catgccggac aacagcatgc
    14341 cggcgagcca ggtgaccagg acgctggcac ccatcgacgc catccatgcg ccgacgactc
    14401 ccggggaaag ctggtccgac cgttcggtgt tgatcccgcc gtaggccgcg agtgggccga
    14461 ccgtcgccag cagcgagatg atctgcatgg ccacgaccac catcgcggtc agtcccagcg
    14521 tcgccttggg gttggcccgg atgtaaccga ccgcgccgtt gaaaatctcg ctgagggtga
    14581 ggggacgcag cgggatgatc ccgggtttga gcgctcccgg gacaactggc gacgggccgt
    14641 agcccggcgg ggggccgtag ccgggaggcg ggccgtaccc cggcggcgca ccgtagcccg
    14701 gcggggggcc gtagccgagt ggggggccgt agccgggtgg ggggccgtag cccggcgggg
    14761 gcccgtatgc cggcggcgga tagccggcgg gcacgtagcc tccaggcgga ccaagcggat
    14821 atccaggcgg cggcgcgttc accgtgtgca gcggccttgc aaaccagctc tggaacaagc
    14881 accgcaaccg ggcccgtcgt tcgtcatgag ctccatcctg tcgacggtcc gggcatttct
    14941 caatcttgac cggtgtcccg tcgcgtagcg tctcgggcat ggcggaactc aaatcccggc
    15001 tccgggcgga cctgaccaag gcgatgaaga accaggacaa gttgcggacg gcgaccctgc
    15061 gcatggtgct ggccgcaatc cagaccgagg aggtctccgg caagcaggcc cgcgagctct
    15121 ccgatgacga ggtcatcaag gtgctggcca gggaatcgcg aaagcgcgcc gaggcggccg
    15181 agatctacac ccagaacggc cgcggcgagc tggccgccga ggaacatgcc gaagcgcggg
    15241 tcatcgacga gtacctgccg actcagctca ccgaggccga ggtggccgac gtcgccgaca
    15301 ccgcgatcgc acaggttgcc gaggaactgg gagagcggcc gggcatgaag cagatgggac
    15361 aggtgatgaa ggccgccacc gcgatcgctg ccggcaaggc cgacggcgca cgcctatccg
    15421 cggcggtcaa ggagcggctg taatttcaat tgctgtgcgc tgaccggtaa tccgcccgag
    15481 cgggattacg acaccacccg gacaacggct ttatcatcca ggcatgctga tccgggtccg
    15541 gtcgccccgg caaattctgg agctgttgcg cagcaaggat ccggagcgcg acgcgctgcg
    15601 ccgcgccatc cgtgcggcca tcatggtgcc gtttacggtg gggttcgcgt ttgtcgtcgt
    15661 gggcggtaag gcgacaccgc tgtacgcgtt gctcggggcc ttctggctgc tggtggtcac
    15721 cgaatttccg ggcaaccgac agcagcgtgc cgtggcctac ctcggcttgg ccctgaacgg
    15781 gtttgtgttg atcaccctcg gcaccctggt cgctcccatc gcctggctgg cggtgacgtt
    15841 gatgtttctc ctcggggtgg cggtgacact ggccggcatg ctcagcgcga cgatgtccgc
    15901 cggccaacgc gcgaccctgt tgttctacgt ctggccggtc tgcacgcccg tcggtccgat
    15961 cggcgagcgg ctgctgggct ggctgatcgc gctggtgctt tgcgtgccgg cggcgttgtt
    16021 cttccttccc ccgcggcact acgacgacct gcgccgccac gccgcacgtg tgtgtgcggc
    16081 gctggccgac cggatcgagg gggtctgctc gaccatggat gtgacggcgg cgatggacgc
    16141 gctgcggtcc aacttcctgg ccgcgaactt ccggccggtc ggtctgagcg ccggcagccg
    16201 ggccctggta cgcatcgtcg actttctcga gctggtcgcc gacctcgtcg acgacgacgc
    16261 cggcaccacg ctggggccgg tgaaaccgcc ggccgtcgcc gtcttgcggt gctgttccgg
    16321 cctgctcgac gcatcccaac tggcccaacg cgcgaccaat cgcgccggtc tcgaacagga
    16381 acttccccga ttgcggtcct tggcacgcgg ccgttaccgc gacgacgtgc tgctgatgct
    16441 cagcgcgccg gacgatgcgg gggcggttga ggttggcgag cggctgctcg cgtgccgagt
    16501 gatcgcgaca accatcggtt tgacgggcag ggtcatcgcc gccgctgcca cggccgatgc
    16561 ccggccggtg tgggcgcggg ccctcgggct acagctgccg cccaccggct tcgccgatcg
    16621 gttgtcacca gaggccgtcg ccggcaccac gatcgtcacc gcgtttctgg ccagccgttc
    16681 cgtagcggca cacaacggcc tgcgcatcgg ggtcgggttg gcgctggcag tcgccctcac
    16741 tcatctgcat ctggtcgagc gcggcttctg ggtcgtggtg ggaacgatgc tggtgatcag
    16801 cagcagtgcg ttggcaacca gaaccaaagt ggttcaggcg gtcgtgggta cggcgctggg
    16861 gatcatcttc ggcgggttgc tcgtcgccgc tgtcggtgtc gcacccgtgg cgcagtggct
    16921 gctggtgccg atcacggtgt tcctggcaac gtacctgccg cgtttcgcgt ccttcaccat
    16981 cgcacaggcc atgggcgcgt tgaatctgct caccatcctg agcctcacca tcccctccgg
    17041 ctggcacctc ggtctgcttc gcatcgaaga catcgcgatg ggcgcggcgg tgggtatggg
    17101 cgtatcgctg ctgttgtggc ccaggggtgc gaccgccgtg gtgtccaagc tgatcggggc
    17161 cgcccgagag gtcaacgtgc gctatctgca ggcggcggta ctgcgtgtca cccgtgatca
    17221 tccggaggaa ggccccgagg aaaccgatgc cgccctcgcg gagctgagcc accatgcggt
    17281 ggtgggcagc cgaagggtcg atgacgctgt gcgccactat ctttcggaga ccggcagcgg
    17341 ggctgacctg cggaccccgg tggtgcgggc agccaaccgc gccacctggg tgcgcctcac
    17401 ggcggacatg atcgccgaca tcaggacgct gccgccgacc ggggcgtacc cgtcggcccg
    17461 ggctttgctg gaagcccacc tggaattcgc caccaaccag gtgttcgggc gtgtcgagga
    17521 gataaatcgg ccgatagcag gggaattcgc gcgggcattg cgcgtcgagg cgacgaacca
    17581 gggcgaagcg gtcgaggccg cgcaggcgct cgtcacggtg gcggccaacc tcgctcaact
    17641 cgagatggtc actgccgtgg aatcggaaac c
//
LOCUS       DASSGQ010000050         2803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1492359,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000050 DASSGQ010000000
VERSION     DASSGQ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492359"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VFI55_02220"
     CDS             <1..454
                     /locus_tag="VFI55_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET7072884.1"
                     /translation="YPPNLYVVDGGAPQVNAANAVLEELGVTDVAVIGLAKRLEEIWV
                     PSEPDPVIMPRNSEGLYLLQRVRDEAHRFAITYHRSKRSKRMTASALDAVPGLGEHRR
                     KALVTHFGSIARLKEATVDQITAVPGIGVATATAVLEALRANQPGVSQ"
     gene            451..1374
                     /gene="rapZ"
                     /locus_tag="VFI55_02225"
     CDS             451..1374
                     /gene="rapZ"
                     /locus_tag="VFI55_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET7072885.1"
                     /translation="MTGQGDGEQFAAFADTHPVQAGIDVVLVTGLSGAGRGTAAKVLE
                     DLGWYVADNLPPQLITRMVDFGLAAGSRITQLVVVMDVRSRGFTGDLDEVRTELATRK
                     IYPRVVFMEASDDMLVRRYEQNRRSHPLQGEQTLAEGIAAERRMLAPVRATADLIIDT
                     STLSVRGLRESIERAFGGDTGASTSVTVESFGFKYGLPMDADIVMDVRFLPNPHWVDD
                     LRPLTGQAPAVADYVLSQPGATEFLDAYHRLLNLVLEGYRREGKRYMTVAIGCTGGKH
                     RSVAIAEALMRRLGSNPQLAVRVLHRDLGRE"
     gene            1371..2459
                     /locus_tag="VFI55_02230"
     CDS             1371..2459
                     /locus_tag="VFI55_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HET7072886.1"
                     /translation="MSPSNSEGIVALGGGHGLYATLSAARRLTPYVTAVVTVADDGGS
                     SGRLRSELDVIPPGDLRMALAALASDSPYGRLWATILQHRFGGNGALAGHPIGNLMLA
                     GLNEVLADPVAALDELGRILGVKGRVLPMCPIALEIEADVSGLETDPRIFRLIRGQVA
                     IATTPGKVRRVRLHPPNPPATRQAVDAIMAADLVVLGPGSWFTSVIPHVLVPDLAAAL
                     RSTPARRAVVLNLVAEPGETAGFSVERHLHVLVQHAPGFTVHDIIIDAERVPGEQERE
                     QLRRSATSLEAEVHFVDVARPGTPLHDPGKLAAALDGVRAGRSRGSKGPGAPSVAATA
                     EVRVDGGSPQTGVKGPTGSGPRGDDAWR"
     gene            2456..>2803
                     /gene="whiA"
                     /locus_tag="VFI55_02235"
     CDS             2456..>2803
                     /gene="whiA"
                     /locus_tag="VFI55_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HET7072887.1"
                     /translation="MTTEVKDELSRLAVKSVSARRAEVTSLLRFAGGLHIVGGRVVVE
                     AEVDLGNTARRLRKEIFELYGYNAVVHVLSASGIRKNTRYVLRVANDGEALARQTGLL
                     DMRGRPVRGLPAQV"
BASE COUNT          458 a          896 c         1014 g          435 t
ORIGIN      
        1 gtacccgccc aacctctatg tcgtcgacgg cggcgcgccg caggtcaacg cggccaacgc
       61 ggtgctcgag gaacttggtg tcaccgacgt cgcggtgatc ggcttggcca agcggctgga
      121 agagatatgg gtgccgtccg agccggaccc ggtcatcatg ccgcgcaaca gcgaggggct
      181 ctatctgctg cagcgggtgc gtgacgaggc gcatcggttc gccatcacct accatcgcag
      241 caagcgatcc aagcgaatga ctgcgtcagc gctggatgcg gtgccaggat tgggagaaca
      301 tcgccgcaaa gcgctggtga cccatttcgg atcgatagcc cgcctcaagg aggccaccgt
      361 cgaccagatc accgcagttc cgggtatcgg cgtggctact gccacggccg tcctcgaggc
      421 gctgcgagcc aatcaaccgg gagtctcaca atgaccggcc aaggggacgg cgagcagttc
      481 gccgcgttcg ccgacactca ccccgtgcag gcaggcatcg acgtcgttct ggtgacggga
      541 ttgtccggcg ccggacgggg taccgcggcc aaggtgctgg aggacctcgg ctggtatgtg
      601 gcggacaacc tgccgccgca gctgatcacc cggatggtcg acttcgggtt ggccgccggg
      661 tcgcggatca cccagctggt ggtggtgatg gacgtgcgtt cgcgcgggtt caccggcgac
      721 ctcgacgagg tgcgtaccga gctggccacc cgcaagatct acccccgcgt ggtgttcatg
      781 gaggcatcag acgacatgtt ggtgcgacgc tacgaacaga atcgccgcag ccacccactg
      841 cagggcgagc agacgctggc cgagggcatc gccgccgagc gcaggatgct ggcgccggtc
      901 cgcgccaccg ccgacctgat catcgacacg tcgacgctgt cggtgcgcgg actgcgggaa
      961 agcatcgagc gcgcgttcgg cggggacacc ggcgcctcca ccagcgtcac cgtcgagtcg
     1021 ttcggcttca aatacgggct gccgatggac gccgacatag tgatggacgt gcggtttctg
     1081 cccaacccgc actgggtgga cgatttgcgt ccacttacgg gccaagcccc ggcggtggct
     1141 gactatgtgt tgagccagcc gggcgccacc gagtttctcg acgcctacca tcgactgcta
     1201 aacctggttc tcgagggcta ccgccgggag ggcaagcgtt atatgacggt cgccatcggg
     1261 tgcaccggag gcaagcatcg cagcgtcgcg atcgccgagg cgttgatgcg gcggctgggg
     1321 agcaatccgc aattggcggt gcgggtactg caccgggatc tgggtcgcga atgagcccgt
     1381 cgaatagcga gggcatcgtt gcgctgggtg gcggacacgg tttatacgcg acgctgtccg
     1441 cggcgcgccg gctgactccc tacgtcaccg cggtggtcac ggtcgccgat gacggtggct
     1501 catcgggccg gctacgcagc gaactggacg tgatcccacc gggcgacctg cgaatggcac
     1561 tggccgcctt ggcatccgac agcccgtatg gacggctgtg ggcgaccatc ctgcagcacc
     1621 ggttcggtgg caatggtgcc ctggccggac acccgatcgg caacctgatg ctggccggcc
     1681 tcaacgaggt gctggccgat ccggtcgccg cgctcgacga actcggacgc atcctcggcg
     1741 tcaaaggcag ggtgttgccg atgtgcccga tcgcgctgga gatcgaggcc gacgtgtcgg
     1801 gcctggagac cgacccgcgg atcttccggc tgatccgcgg ccaggtggcg atcgcgacca
     1861 cacccggcaa ggtgcggcgg gtgcggctgc atccgccgaa cccgccggcg acccggcagg
     1921 ccgtcgacgc gatcatggcg gccgacctgg tggtgctggg gcccgggtcg tggttcacca
     1981 gtgtgatccc gcacgtgctg gtgccggacc tggcggcggc gctgcgcagc acgcccgcca
     2041 ggcgcgcggt ggtactgaat ctggtggccg aaccggggga gacggccggt ttctccgtcg
     2101 agcgtcatct gcacgtgctg gtccagcacg cgccgggctt caccgtgcac gacatcatca
     2161 tcgacgcgga acgggtgccg ggggagcagg aacgtgagca actgcgccgc tccgcgacct
     2221 cgctagaagc cgaggtccac ttcgtcgatg tggcccgacc tggtacacct ttacatgacc
     2281 ccggcaagct cgcggcggcg ctcgacgggg tccgagcggg tcgcagtcgt ggttcaaaag
     2341 gcccgggagc cccctcggtg gcggccaccg cggaggttcg ggtcgacggt ggaagtccac
     2401 agaccggcgt gaagggaccg actggcagcg gaccgagggg tgacgacgcg tggcgatgac
     2461 gaccgaagtc aaggacgagc tgagccggct ggcagtgaaa tcggtcagcg cgcgccgcgc
     2521 cgaggtcacg tcgctgctga ggttcgccgg cgggttgcac atcgtgggcg gccgggtggt
     2581 ggtcgaggcc gaggtggacc tgggcaacac cgcgcggcga ctgcgcaagg agatcttcga
     2641 actctacgga tacaacgccg tggtgcatgt cttgtcggcc agtgggattc ggaagaacac
     2701 ccgctacgtg ctgcgggtgg ccaacgacgg cgaggcgctg gcacggcaga ccgggctgct
     2761 ggacatgcgg gggcgtccgg tgcgcggcct gcccgcccag gtc
//
LOCUS       DASSGQ010000051         4200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_216221,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000051 DASSGQ010000000
VERSION     DASSGQ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216221"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1360)
                     /locus_tag="VFI55_02240"
     CDS             complement(17..1360)
                     /locus_tag="VFI55_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET7072888.1"
                     /translation="MSAATTSDPELRRVFVLDTSVLLADPGALGRFGEHDVVLPLVVI
                     NELEGKRHHPELGWFARETLRLLDDLRVQEGRLDAPVPIGDDGGRLHVELNHADFSRL
                     PTGFHLDNNSARILAVALNLAAEGRDVTLVTKDMPLRVKAASVGLRADEYRVHATVDS
                     GWTGMAEVPVSAETVGALYAADRVPLVASEYEDLAAANDLPCHTGLVLQSPRGSALAR
                     ITPDKQVRLIRGDREAFGLRGRSAEQRVALDLLLDPDIGIVSLGGRAGTGKSALALCA
                     GLDAVLERRAHKRVLVFRPLYAVGGQELGYLPGTENEKMAPWAQAVFDTLGALVSQNV
                     IDEVLDRGMVEVLPLTHIRGRSLHDSFVIVDEAQSLERNVLLTVLSRIGTGSRVVLTH
                     DVAQRDNLRVGRHDGIAAVIDALRGHPLFAHVTLTRSERSPIAALVTEMLEYLPW"
     gene            complement(1748..2515)
                     /locus_tag="VFI55_02245"
     CDS             complement(1748..2515)
                     /locus_tag="VFI55_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013127729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET7072889.1"
                     /translation="MGVRSAVYSIYERRLRSQLAGKPIPHHVAVMLDGNRRWARAAGF
                     EDVTQGHVAGARRISDLLEWCTEAGVEHVTIWLLSTDNLTRPAGELEPLLRIIEGVAA
                     ELSDDGRPWRINPVGALDLLPEATANALKAAAERTSTRRGLTVNIAVGYGGRREIADA
                     VRALLQEHAAAGSSLEEVAEFLDVDHIAEHLYTRGQPDPDLLIRTSGEQRLSGFLLWQ
                     SAHSEFYFCDALWPDFRKVDFLRALRAYGTRQRRYGG"
     gene            2752..3447
                     /locus_tag="VFI55_02250"
     CDS             2752..3447
                     /locus_tag="VFI55_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005199257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072890.1"
                     /translation="MRYENTSDAERRHHHPGGHGHGPGFSGFGRGGGYRRGRGRGFGR
                     PGGWQQADLPLTDDLTAWLAGRLPDDWFTGAPQVTVDREEITVIGELPAPAASPEGGD
                     GASAEGRISRFREETREKRMQVADEAEARYGRKVAWGASIGDTTVLFTHLSVPVMTRL
                     RQSERRVLDTLVDAGVARSRSEALAWSVRLVGEHTEEWLSSLRSAMSEVDKLRAEGPD
                     LSGEADTPGTTEV"
     gene            complement(3471..>4200)
                     /locus_tag="VFI55_02255"
     CDS             complement(3471..>4200)
                     /locus_tag="VFI55_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acylglucosamine 2-epimerase"
                     /protein_id="HET7072891.1"
                     /translation="AAVRCADLLVERHLVGGRLRRVSRDGRVGEALGVAEDYGDLAEG
                     LLALHQATGSADYLRVAESLLNTAVEKFGDGRGGFYDTADDAEALIQRPQDPTDNATP
                     SGSSALAGALLTSAALTGSLVHLQSAQSALGIVAAVGVEQPRFLGWALAVAEALVSGP
                     VEVAIVGEPSRALRDVAWRLRPPGAVVVSGQPDAPGIPLLADRPTIDGRPAAYVCRGM
                     VCDAPVTDVEALRRQLAEAAPASL"
BASE COUNT          764 a         1432 c         1392 g          612 t
ORIGIN      
        1 gcgggcgaat agccaactac cagggcagat actcaagcat ctcggtaacg agcgccgcga
       61 tcggcgaccg ctcggaccga gtgagggtca cgtgtgcgaa cagcggatgc ccgcgtagtg
      121 cgtcgatcac ggcggcaata ccgtcgtgcc gacccacccg caggttgtcg cgctgcgcca
      181 catcgtgggt cagcacgacc cttgagccgg tgccgatccg agacagcacg gtgagcagca
      241 cgttgcgctc gagggactgt gcttcatcga cgatcacaaa cgagtcgtgc aatgaccgtc
      301 ctcgtatgtg ggtcagtggc aggacttcga ccatgcctcg gtcgagcacc tcatcgatca
      361 cgttctggct gaccaacgcg ccgagcgtgt cgaataccgc ctgcgcccat ggcgccatct
      421 tctcgttctc ggtgccgggc agataaccaa gctcctgccc acccacggcg tacaacggcc
      481 gaaacaccag gacgcgctta tgcgcgcgcc gctcaaggac cgcatccaga cctgcacaca
      541 acgcgagcgc agacttgccg gtgccggctc ggccacccag cgacacgatg ccgatgtcgg
      601 gatcgagaag cagatcgagc gcgacgcgtt gttcggccga acgaccgcgc aggccgaacg
      661 cctcccggtc gccccgtatg aggcgaacct gcttgtcggg cgtgatccgc gcaagtgccg
      721 agccccgcgg cgactggagc accaatccgg tatggcacgg aagatcgttt gcggcggcca
      781 gatcttcata ctcgctggcg accagcggaa cccggtccgc ggcgtatagg gcaccgacgg
      841 tctcggccga taccgggact tccgccatgc ccgtccaacc ggaatcgacg gtcgcatgaa
      901 cgcgatactc gtcggcgcgc agcccgaccg acgccgcctt gacccgcagc ggcatgtcct
      961 tcgtgaccaa cgtgacgtcc cgaccctcgg ccgcgagatt cagcgcgaca gccaggatgc
     1021 gggccgaatt gttgtcgagg tgaaagcccg tcggcaggcg agagaagtcg gcatggttca
     1081 gctcgacatg tagacgtccg ccgtcgtcgc cgatcggtac cggcgcatcg agccgccctt
     1141 cctgcacgcg cagatcgtcg agcaacctga gcgtttcccg agcgaaccag cccagctcgg
     1201 ggtggtgacg cttgccctcc agctcattga tgaccacgag tggcagcaca acgtcgtgct
     1261 ccccgaatcg gccgagcgct cccgggtccg cgagcagcac ggaggtatcc aggacgaaca
     1321 cgcggcgaag ctcaggatca gacgtggtgg cggcgctcat gggacgctcc agatggggtc
     1381 ggcgggtggc acgatgcggt aaggcgtagc accagatatc ggcacgacga gactccgtcg
     1441 aggatccggc ggcagcaccg cccaacgtcc agcgggcgcc ggtccggccc ggtcgctgat
     1501 cgtcggcacg gtgcgggacc tcccgggcgg tccggttggc cggatcacca ccgagacacg
     1561 ctaccggcca cgccagggcc ccgggtgccc ggcacgcggt ttggcgccga ttcgggcaga
     1621 ttccgccaag agcctgcgcc agtagcgcgc catagccgac cggggtttcc acgcgaaatc
     1681 acgcagcgac ctgggtccct gcgaaatcgc gccagcgatc cgggcgctaa gtgcagggcg
     1741 agcgcgctca gccgccgtag cggcgttgcc gagttccgta ggcgcgcagc gcgcgaagga
     1801 agtcgacctt gcggaagtcc ggccacagcg cgtcgcagaa gtagaactcc gaatgggccg
     1861 actgccacaa caggaagccc gacagccgct gttcccccga ggtgcggatc agtagatccg
     1921 gatccggctg gccccgggtg tagagatgtt cggcgatgtg atcgacgtcc aggaactcgg
     1981 cgacttcctc cagtgagcta cccgcagcgg cgtgctcctg aagcagggcg cgcaccgcgt
     2041 cggcaatctc gcgtcgtccc ccgtatccga cggcgatgtt cacggtcagt cctcgacggg
     2101 tcgaggtgcg ctctgcggcc gccttcaacg cgttggcagt ggcttcagga agcagatcca
     2161 gcgccccgac cgggttgatc cgccacggcc ggccgtcgtc cgatagttcg gccgcgacac
     2221 cttcgatgat ccgcagcaac ggctccagct cgccagccgg ccgggtgaga ttgtccgtgg
     2281 agagtaacca gatcgtgacg tgctcgacgc cggcctcggt gcaccattcg agcaggtcgg
     2341 agatgcgcct cgcgccggcg acgtgaccct gggtcacatc ctcgaatccg gctgcccgcg
     2401 cccagcgacg gtttccgtcg agcatgaccg caacgtgatg cgggatgggc tttccggcga
     2461 gctgactacg gagccgacgt tcgtagatgg agtagacagc gctacggacg cccacgatca
     2521 cgcagcctag acccgccagc ccagcagccg gcccgacgtg gcagatcgtc gcgcagcgca
     2581 gtgcgggcgg gaacggactt atccggcata gcggcgccgt cgaaatcctt gctgtcccat
     2641 tcgccgcgtt cgacctatta accccgtgta gtggattcgg gaatacgagc actggacgtc
     2701 aggttgccac gtatgcaagt atgtaatcat gtaatttaga aggagcaaga aatgagatac
     2761 gagaacacat ccgatgccga gcggcggcat caccaccccg gtggccacgg ccacggaccc
     2821 ggtttcagcg ggttcggacg cggtggcgga taccggcgtg ggcgcggccg tggtttcggc
     2881 cggcccggtg gctggcaaca agccgatctg ccactaaccg acgacttgac cgcgtggctc
     2941 gccggacgcc tacccgatga ctggttcacc ggcgcaccgc aggtgaccgt tgaccgggag
     3001 gaaatcaccg tcattggcga gctgccggcc cccgcggcgt cgcccgaggg cggcgatggc
     3061 gccagcgccg aagggcggat aagccgcttc cgcgaggaaa cccgcgagaa gcgaatgcag
     3121 gtcgcggacg aagcggaagc gcgctacggc cgcaaggtgg cctggggagc gtcgatcggc
     3181 gacaccacgg tgctgttcac gcacctgtcg gtgcccgtca tgacgcgcct gcgccagagt
     3241 gagcggcgcg tgctcgacac gctggttgac gccggtgtcg cgcgctcccg gtccgaggcg
     3301 ctggcctggt ccgtgcgcct ggtcggcgag cacaccgagg agtggctcag cagcctgcgc
     3361 agcgcgatgt ccgaagtgga caagctgcgg gccgaagggc cagatctgag cggtgaggcc
     3421 gacacgcctg gcaccaccga ggtctaaacc aacatcaccc cgcgccggcg ctacaaactc
     3481 gccggcgcgg cctccgcgag ttggcgacgc aacgcctcga cgtcggtcac aggcgcatca
     3541 cacaccatgc cgcggcagac ataagcagct ggccgaccgt caattgtcgg gcgatcggcc
     3601 aacagcggga tgcctggcgc atctggttgc ccactgacca caaccgcccc gggcgggcgc
     3661 aaccgccaag caacatcgcg gagggcgcgc gatggttcgc cgacgatagc cacctccacc
     3721 ggcccgctga ccagtgcctc agccacggcg agggcccagc cgaggaagcg gggctgttcg
     3781 acgccgactg ccgcaacgat tcccagagca gactgcgccg actgcaggtg tacgagcgaa
     3841 ccggtgagag cggcagaggt caacaacgcc ccggccagtg cgctcgaccc cgacggggtg
     3901 gcattgtccg tcggatcctg cggccgctga atcaacgcct ccgcgtcgtc agccgtgtcg
     3961 tagaagccac cccgtccgtc gccgaatttc tcgacagccg tgttgagcag cgattcggcg
     4021 acgcgtaggt agtccgccga tcccgtcgcc tgatgcaggg cgagcagccc ttcggccagg
     4081 tcgccgtaat cctcggctac gccgagtgcc tcaccgaccc gaccatcgcg agatacccga
     4141 cgcagtctcc cgcccaccag atggcgctcg accaggagat cggcgcagcg cacggccgcg
//
LOCUS       DASSGQ010000052         7179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1249473,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000052 DASSGQ010000000
VERSION     DASSGQ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249473"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VFI55_02260"
     CDS             <1..305
                     /locus_tag="VFI55_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET7072892.1"
                     /translation="GSRLVAEVFLNSGANLEALNAATQKWRENGLDVALDNLGFPGDR
                     NDVAGYLQDRGWHPARTPLNQLLADNGLPLQSTGPDAESAPFSMNYYCTAVLDKAA"
     gene            319..1527
                     /locus_tag="VFI55_02265"
     CDS             319..1527
                     /locus_tag="VFI55_02265"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET7072893.1"
                     /translation="MPNSKPAKPLDGFRVLDFTQNVAGPLAGQVLADLGAEVIKVEAP
                     GGDAARHITAVLPGRPPLATYFLPNNRGKKSVTADLTTEEGKQRILGLADTADVVLEG
                     FRPGVMERMGLGPDDLRSRNPKLIYARLSAYGGNGPHGSRPGVDLMVAAEAGMTTGMP
                     TPDGKPQIIPFQLVDNASGHVLAQAVLAALLNRERHGVADTVRVAMYDVAVGLQANQL
                     TMHLNKPGDDQPKQDSGSAAKPNAKKRKGVGFATQPSDAFRAADGYLVISAYVPKHWA
                     KLCEIVGRSDLLQDERFLSQRSRALNYPELTDELESALAAKPAAEWVELLQEGGLMAC
                     LAYTWKQVVDTPLFAENDLALHVGAGDEAITVIRTPARFSSFDAVATDPPPAAGQHND
                     LFLTTPEAVS"
     gene            complement(1540..3474)
                     /locus_tag="VFI55_02270"
     CDS             complement(1540..3474)
                     /locus_tag="VFI55_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase PknD"
                     /protein_id="HET7072894.1"
                     /translation="MNDTESRVGTRFGPYQLRRLLGRGGMGEVYEAEDTRKHRIVALK
                     LMSPQFSTDTTFRARMQREADTAGRLTEAHIVPIHDYGDIDGQFYVEMRMIEGVSLRT
                     LLTNFGPLGPARTVAIVRQIAAALDAAHANGVTHRDVKPENILITNDDFAYLVDFGIA
                     RAATDPGLTQGGIAVGTYNYMAPERFTGDQVTYRSDIYALACVLGECLTGAPPYRADS
                     VERLIAAHLLEPAPRPSQLRPGRVPPELDPVIGKGMAKNPADRYFTAGDLAAAAHSAL
                     SPPEQLQEATILRQGENATLLANATDRAWTSYAGSGADLTMARPVPWRDSGGSRRHTG
                     PPAPPAFGQQQSVLSPVAPRKRKRWILAGAAVLLLAAAVTATGYLGIHPSAASKQQVS
                     GQIELPFNGIDFRLSPGGVALDKAGDVYVTSEGMYGRVVELPPGSSTPTVLPFSGLYQ
                     PQGLAVDNAGAVYVADFDNRVVKLTAGSTNQTVLPFTGLDYPEGVAVDGQGSVYVADR
                     GNNRVLKLPPGSNTATALPFTGLKNPDGVAVDPAGNVYVTDTDNNRVLMLAAGATAAN
                     VLPFTGVAVPWGIAVDNAGDVFVTEHDSSQVLKLPAGSNNPTVLPLTGLNTPLGVAVD
                     QNGSVYVDDRGNDRTVKLAA"
     gene            complement(3542..4648)
                     /gene="pstS"
                     /locus_tag="VFI55_02275"
     CDS             complement(3542..4648)
                     /gene="pstS"
                     /locus_tag="VFI55_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET7072895.1"
                     /translation="MKSNRAGAVLGLLAAGALVLAGCGSTTHASSSGATAAPVQCGGK
                     KQLKGSGATAQENAMEQFVFAYVHACPGYTLDYNANGSGAGVTDFVNNVSDLAGSGDP
                     LDPSKGQVDRAAQRCGSPAWDLPTVFEPIAITYDVSGVNTLKLDAPTLAKIFNGGISK
                     WDDPAIRALNPGANLPAEPIHVLFRSDQSGDTSNFEQYLVGASDGAWGRGAGQAFLGG
                     VGDGGVGDNGTSAQVQSTDGSITYNEWSFAVGRQLNMAQIITSAGPDAVSITTDSVSK
                     TIAGATFKGQGNDLVVDTSSFYQPTQPGAYPIVEPTYEIVCSKYPDSATGAAVKAFMQ
                     AAIGPGQVGLDQYGSIPLPSAFQSKIAAAVNAIS"
     gene            5049..5873
                     /locus_tag="VFI55_02280"
     CDS             5049..5873
                     /locus_tag="VFI55_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7072896.1"
                     /translation="MTAVNLALSFGTKTVLDEVSLDFPTRAVTCLLGPTGSGKTTFLR
                     TLNRLNDKVSGYRYHGDVLLGGHSIFKSSDLMGFRRRVGMLFQRPNPFPMSIVDNVLA
                     GVRAHKLVPRKEFKAVAQARLTEVGLWDAVKDRLGDSPFRLSGGQQQLLCLARALAVH
                     PQVLLLDEPTSSLDPATTENIEKLIRSLANRLTVIMVTHDLAQAARIGDRTALFFGGR
                     LIEEGPTAQLFLAPRHAETVRYVSGLFGDPGLPGPESGPESGCVGANLISDGGSER"
     gene            5880..6995
                     /gene="pstS"
                     /locus_tag="VFI55_02285"
     CDS             5880..6995
                     /gene="pstS"
                     /locus_tag="VFI55_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003900236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET7072897.1"
                     /translation="MKFRLRALLVAVPVMLVAAACGANSAGGSSSPGPRGGSPATSPA
                     TSKVALSETGASELYPLMNLWAPAYNAKYPNVSITTGSTGSGAGISQAAAAAVNIGAS
                     GAYLSGGDMSAHKGLMNIALAVSAEQVNYNLPGITEHLKLNGKVLAAMYEGTIKTWND
                     PQIAGLNPGVNLPATPVVPLHRSDGSGDTFVFTQYLSKQDPDGWGKSPGYNTTVDFPA
                     VPGALGENGAGGMVTGCAQTPGCVAYIGTSYLDQAAQKGLGAAQLANASGNYLLPDAH
                     SVSAEAAGFAAPTPANEVISLINGPAPDGYPIVNYEYAVVYANQKDPAVAQTIQAFLH
                     WTVTDGNSPSFLDQFHFQPLPASVQKLSDAQIATITT"
     gene            7074..>7179
                     /locus_tag="VFI55_02290"
     CDS             7074..>7179
                     /locus_tag="VFI55_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET7072898.1"
                     /translation="MVRWAGGVAAVIPLLALVFVVVTLLIEATGAIRFN"
BASE COUNT         1208 a         2431 c         2335 g         1205 t
ORIGIN      
        1 acggcagccg gctggtggcc gaagtctttc tgaattccgg tgccaacctg gaagctttga
       61 acgctgccac ccagaagtgg cgggaaaatg gacttgacgt cgccctggac aatctgggtt
      121 ttcccggcga ccgcaacgac gtggcggggt acctgcagga ccgcggctgg catccggctc
      181 gcacgccgct gaatcaactg ttggccgaca acggactacc cctgcagtcg accggccccg
      241 atgctgaaag tgcgccgttt tcgatgaact actactgcac cgcagtgttg gacaaggcag
      301 cttaagggaa ggcacagcat gccgaacagc aagcccgcca agccacttga cggattccgg
      361 gtactcgatt tcacccagaa cgtagccggg ccgctggccg gacaggtgct ggccgacctg
      421 ggcgccgagg tgatcaaggt cgaggcgccc ggcggcgacg cggcccggca tatcaccgcg
      481 gtcctgcccg gacgcccgcc gttggccacc tacttcctac cgaacaaccg gggcaagaag
      541 tcggtgacgg cggacctgac caccgaagaa ggcaagcagc ggatactggg gctcgccgac
      601 accgccgacg ttgtgctgga agggtttcgg cccggggtca tggaacgcat ggggttgggc
      661 cccgacgact tacggtcgcg caatcccaag ctgatctacg cgcgcctgtc cgcctacggc
      721 ggcaacggcc cacacggcag caggccgggc gtcgacctga tggtggccgc cgaggcgggc
      781 atgaccaccg ggatgcccac gccggacggc aaaccgcaga tcatcccgtt ccaactcgtc
      841 gacaacgcca gtggccacgt gctggcccag gccgtgctgg ccgcgctgct caaccgtgaa
      901 cgccacggcg tggccgacac ggtccgggtc gcgatgtacg acgtcgccgt gggactacag
      961 gccaaccagc tcacaatgca cctgaacaag ccgggcgatg accaacccaa acaggacagc
     1021 ggttcggcgg caaagccaaa tgccaagaag cgcaagggag ttggttttgc cactcagccg
     1081 tccgatgcgt tccgggccgc cgacggctac ctcgtgatca gcgcatacgt ccccaaacac
     1141 tgggccaagc tgtgcgagat cgtcggccgg tccgatctgc tgcaggacga gcggttcctc
     1201 agccagcgct cgcgggcgct gaactacccc gagctgaccg acgaactcga atcggcactg
     1261 gcggccaagc ccgccgccga atgggttgag ctgctgcagg aaggcggcct gatggcctgc
     1321 ctcgcctaca cctggaaaca ggtcgtcgac accccgctgt tcgccgagaa cgatctcgcc
     1381 ctgcacgtcg gcgccggcga tgaggccatc acggtgatcc gcaccccggc ccgcttttcc
     1441 agcttcgacg ccgtggccac cgatcccccg cccgccgcgg gacagcacaa tgacctgttt
     1501 ctcaccacac ccgaagcggt ttcataaccc tgtggcacct caggccgcca gcttgaccgt
     1561 tcggtcgttg ccgcggtcgt ccacgtagac actgccgttc tgatcgaccg ccacccccag
     1621 gggcgtgttg aggccggtca acggcagcac cgtcgggttg ttggagccgg ccggcaactt
     1681 cagtacctgg ctgctgtcgt gttcggtgac gaacacgtcg ccggcgttgt ccaccgcgat
     1741 gccccacggc acggcgacgc cggtgaacgg cagcacgttc gccgccgtcg ccccggccgc
     1801 caacatcagc acccggttgt tgtcggtgtc ggtgacgtag acgttgccgg ccgggtccac
     1861 cgccacaccg tccgggttct tcaggccggt gaacggcagc gcggtcgcgg tgttcgaccc
     1921 gggcggcagc ttcagcaccc tgttgttgcc ccggtcggcg acgtagacgc tgccctggcc
     1981 gtccaccgcc accccttcgg ggtaatcgag gccggtgaac ggcagcacgg tctggttggt
     2041 cgacccggcg gtcaacttca ccacgcggtt gtcgaaatcg gcgacataca cggcgccggc
     2101 gttgtccacc gccagcccct ggggctggta gaggccgctg aacggcagca ccgtcggcgt
     2161 gctcgatccg ggcggcagct ccaccacccg tccgtacatg ccttcactgg tgacatacac
     2221 gtcgccggcc ttgtccagcg ccaccccgcc cggagacagg cggaagtcga taccgttgaa
     2281 gggcaactcg atctgtcccg acacctgctg cttggatgcc gccgacgggt ggatgccgag
     2341 gtacccggtg gcggtgacag cggcggccag cagcagcacg gcggcgccgg ccaagatcca
     2401 ccgcttgcgt ttgcggggcg caaccggcga cagaaccgac tgttgttgac caaacgccgg
     2461 cggcgccggt ggcccggtgt gacgccggct accaccgctg tcgcgccagg gcaccggcct
     2521 cgccatggtc aaatcggcgc ccgagccggc gtagctggtc catgcccggt ccgtggcgtt
     2581 ggccagcagc gtcgcgttct cgccctgccg cagaatcgtg gcttcctgca gctgctcggg
     2641 tgggctcagc gcgctgtgcg cggccgcggc cagatccccg gcggtgaaat agcgatccgc
     2701 cgggtttttc gccatcccct tgccgatcac cgggtccagc tccggcggca cccgcccggg
     2761 ccgcagttgg ctgggccgcg gggcgggctc gagcagatgc gcggcgatca accgctcgac
     2821 gctgtcggcc cggtagggcg gcgcccccgt caaacactca cccaacacgc aagccagcgc
     2881 gtagatgtcg gaacggtagg tgacttggtc gccggtgaac cgctccgggg ccatgtagtt
     2941 gtaggtgccc accgcgattc cgccctgggt caggccggga tcggttgcgg cgcgggcgat
     3001 accgaaatcc accaggtagg cgaagtcatc gttggtgatc aggatgttct ccggcttgac
     3061 gtcgcggtgc gtgacgccgt tggcgtgggc ggcgtccagc gcggcggcga tctggcgcac
     3121 gatggcgacg gttcgtgccg ggcccaacgg accgaagttc gtcaacagcg tccgcagcga
     3181 gacgccctcg atcatgcgca tctccacgta gaactggcca tcgatgtcgc cgtagtcgtg
     3241 gatgggaacg atgtgggctt cggtcagcct cccggcggtg tccgcctcgc gttgcatccg
     3301 ggcccggaac gtcgtatcgg tggagaactg cggcgacatc agcttcagcg cgactatccg
     3361 gtgcttgcgg gtgtcctcgg cctcatacac ctcgcccatg ccaccgcgac ccaaaagccg
     3421 cctcagctga tagggcccaa accgcgtccc cacccgtgac tcggtatcgt tcacgtcgac
     3481 gctcctttca ctaattcgcc cggtcgccgt ctattcaagg ggcgcaaatt gagatagccg
     3541 atcacgaaat tgcgttcacg gccgccgcta ttttcgattg gaacgcgctc ggcagcggaa
     3601 tcgatccgta ttggtctaaa cccacttggc ccggaccaat tgcggcctgc ataaatgctt
     3661 tcaccgcggc tccggtcgcc gagtccggat atttcgagca gacaatttca taggtcggct
     3721 cgacgatggg gtaggcgccc ggctgcgtgg gctggtaaaa cgacgaggtg tccaccacca
     3781 ggtcattccc ttggcccttg aacgtggccc ccgcgatggt cttgctgacc gagtcggtgg
     3841 tgatcgacac cgcgtccggg ccggccgacg taatgatctg ggccatgttc aactgccggc
     3901 ccaccgcgaa cgaccattcg ttgtaggtga tcgacccgtc ggtgctctgc acttgtgccg
     3961 aggtgccgtt atccccgacg ccgccatcac cgacgccccc gaggaacgcc tggccggcgc
     4021 ccctgcccca cgcgccgtcg gaggcgccga cgaggtattg ctcgaagttg gacgtgtcac
     4081 cggattggtc gctgcggaac aagacgtgaa tgggctccgc cggcaaattg gcgccggggt
     4141 tgagcgccct gatcgcaggg tcatcccatt tgctgatgcc gccgttgaaa atctttgcca
     4201 gcgttggcgc atcgagtttc agggtgttca cgccgctgac gtcgtaggtg atcgcgatcg
     4261 gctcgaacac cgtcggcaga tcccaggcgg gcgaaccgca ccgctgggcc gcgcgatcga
     4321 cctggccctt cgatggatcc aacggatcgc cagaacccgc cagatcgctg acgttgttga
     4381 cgaaatccgt caccccggcg cccgacccgt tggcgttgta gtccagcgtg tacccggggc
     4441 acgcatgaac gtaggcgaac acgaattgct ccatcgcgtt ctcctgtgcg gtcgcgccgc
     4501 tgcccttgag ctgcttcttg ccgccgcact gcaccggcgc ggcggtggcc ccggaggacg
     4561 acgcgtgggt ggtgctcccg catcccgcta acaccagtgc gccggcggcc agcagcccca
     4621 gcacagcgcc cgctcggttg gacttcaccg gaaactcctc cgactcgtca gggaggcgga
     4681 cgcggtccgc caccggaaag ccccccttga aaaggtgaga acacgttctg ccgcgccgga
     4741 cagacacatg aaccttccga atatgcaagc cgcgcaggat gggcgcaggg ttggcggcgc
     4801 ctatgcggat catcagaaat gggcagagga ttttcgcagc gttttcaaga tgagcggata
     4861 ttaggcaccg gatcggccca cgctttgaaa atggggagag ggtgagcgtt gcctttgaag
     4921 atatgcagag catcaccgcg ggccgccttt atcgcggcat agcgacgtag cttgatgggg
     4981 tgtttaccgg ccttgcaccc ctggacgccg agaccggcgg cgttgatccc gacggcgctg
     5041 ctccggcgat gacggcggtg aatctcgccc tcagtttcgg caccaagacc gtgctcgacg
     5101 aggtgagcct ggacttcccc acgcgtgcgg tcacctgcct gctcggaccg acgggctcgg
     5161 gtaagacgac cttcctgcgc actctgaatc ggttgaacga caaggtttcc ggctaccgat
     5221 accacggtga cgtcctgttg ggcggacaca gcatcttcaa aagctccgac ctgatggggt
     5281 ttcggcgcag ggtcggcatg ctgttccagc gtcccaaccc gttcccgatg tcgatcgtgg
     5341 acaatgtgct cgccggcgtg cgcgcccata agttggtgcc gcgcaaggaa ttcaaggcgg
     5401 ttgcgcaggc ccgcctgacc gaggtcggcc tgtgggatgc ggtcaaggat cggctcggtg
     5461 attcgccgtt ccggctctcc ggtgggcagc agcagctgct gtgtctggcc cgtgcgcttg
     5521 cggtgcaccc ccaggtgctg ctcctcgacg agcccacctc ctcactggac ccggccacga
     5581 cggagaacat cgagaagctg attcgatcgc tggccaaccg gctcaccgtg atcatggtga
     5641 cccacgatct ggcccaggcc gcccggatcg gcgaccggac cgcgttgttc ttcggcggca
     5701 ggctgatcga ggaaggtccc accgcgcagt tgttccttgc gccgcggcat gcggagaccg
     5761 tccgttacgt ctccggactg ttcggcgacc ccggcttgcc ggggccggaa agcgggcctg
     5821 aaagcggttg tgtgggggca aatctgatct cagacggggg cagcgaaagg tgagggccca
     5881 tgaagtttcg tttgcgtgcg ctattagtcg cggtaccggt gatgctggtc gcagccgcct
     5941 gcggcgcgaa ctccgcgggc ggttcgtcca gccccgggcc ccgcggcggt agccccgcca
     6001 cctcccccgc gacgtcgaag gtggcgttgt cggaaaccgg tgctagcgag ctgtatccgc
     6061 tgatgaatct gtgggcgccc gcttacaacg cgaaataccc gaacgtctcg atcaccacgg
     6121 gcagcaccgg gtcgggtgcc gggatctccc aggccgctgc ggccgcggtc aacatcggcg
     6181 cctccggcgc ctatctgtcc ggtggtgaca tgtccgcgca caaggggctg atgaacatcg
     6241 cgctggccgt ctccgccgag caggtcaact acaacctgcc cggcatcacc gaacacctca
     6301 agctgaacgg caaagtgctg gcggcgatgt acgagggcac gatcaaaacc tggaacgacc
     6361 cgcagatcgc ggggctcaac cccggggtga acttgccggc gactccggtg gtcccgctgc
     6421 accggtccga cggctcgggc gacactttcg tgttcaccca gtatctgtcc aaacaggatc
     6481 ccgacgggtg gggtaagtcg cccggctaca acaccaccgt cgacttcccc gcggtgccgg
     6541 gcgctttggg cgagaacggc gccggcggca tggtgaccgg ctgcgcccag acccccggct
     6601 gcgtcgccta catcggcacc agctacctcg accaggccgc ccagaaaggg ttgggcgcag
     6661 cccagctggc caatgcctca ggtaattacc tgctgcccga cgcccacagc gtctcggccg
     6721 aggccgccgg cttcgccgcc ccgaccccgg ccaacgaggt gatctcgctg atcaacgggc
     6781 ccgccccgga cgggtatccg atcgtcaact acgagtacgc cgtggtgtac gccaaccaga
     6841 aggatcccgc tgtggcgcaa acgattcagg cattcctaca ctggacggtc accgacggga
     6901 acagcccctc gttcctggac cagttccact tccagccgct gccggcctcg gtgcagaagt
     6961 tgtccgacgc ccagatcgcg acgatcacga cctaggcggc tccggtgaca ctcacgcttc
     7021 ccgggcaacg gtacgcgccg ggacgccccc cggaagcgag ccgcggggga cgcctggtgc
     7081 ggtgggccgg cggggtggcc gcggtgatcc cgctgctggc gctggtgttc gtggtggtga
     7141 cgctgctcat cgaggcgacg ggcgccatca ggttcaact
//
LOCUS       DASSGQ010000053         4042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_398615,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000053 DASSGQ010000000
VERSION     DASSGQ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398615"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFI55_02295"
     CDS             complement(<1..509)
                     /locus_tag="VFI55_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7072899.1"
                     /translation="MSGHSKWATTKHQKAVKDARRGKEFARLIKNIEVAARTGGGDPA
                     GNPTLYDAIQKAKKTSVPNDNIERARKRGAGEEAGGADWQTIMYEGYAPNGVAVLIEC
                     LTDNRNRAASEVRVAMTRNGGAMADPGSVSYLFSRKGVVALEKNGLTEDDVLTAVLDA
                     GAEDVNDLG"
     gene            complement(673..1269)
                     /gene="pdxT"
                     /locus_tag="VFI55_02300"
     CDS             complement(673..1269)
                     /gene="pdxT"
                     /locus_tag="VFI55_02300"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET7072900.1"
                     /translation="MTTPKIGVLALQGDTREHLAALREAGAESMPVRRRSELDAVDGL
                     VIPGGESTTMTHLLLDFELLEPLRGRLADGLPAYGACAGMIMLASQILDAGARGRQAL
                     PLRAIDMTVRRNAFGRQVDSFEGDIEFAGLDGPVRAVFIRAPWVERAGDGVQVLARAA
                     GHIVAVRQGAALATAFHPEMTGDRRIHHMFVDIVNGKL"
     gene            complement(1277..2122)
                     /gene="tesB"
                     /locus_tag="VFI55_02305"
     CDS             complement(1277..2122)
                     /gene="tesB"
                     /locus_tag="VFI55_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HET7072901.1"
                     /translation="MSIQKILDLEQLEVNIYRGSVFSPESGFLQRTFGGHVAGQSLVS
                     AVRTVDPRYHVHSLHGYFLRPGDAKEPTVFLVERTRDGGSFTTRRVNAIQHGEIIFSM
                     GASFQTPQQGISHQDAMPAAPPPDGLPGLDSVWVYANSGFRQFEEWDVRIVPRDRIQL
                     LPGKASQQQVWFRHRDPLPDDPVLHICALAYMSDLTLLGSAQVNHLDERKHMQVASLD
                     HAMWFMRGFRADEWLLYDQSSPSACGGRALTQGKIFTQSGAMVAAVMQEGLTRYERGY
                     QPETQ"
     gene            complement(2132..3043)
                     /gene="pdxS"
                     /locus_tag="VFI55_02310"
     CDS             complement(2132..3043)
                     /gene="pdxS"
                     /locus_tag="VFI55_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET7072902.1"
                     /translation="MDSAAQSSQRNQTGTARVKRGMAEMLKGGVIMDVVTPEQARIAE
                     GAGAVAVMALERVPADIRAQGGVSRMSDPDMIEGIIAAVTIPVMAKARIGHFVEAQIL
                     QSLGVDYVDESEVLTPADYTHHIDKWKFTVPFVCGATNLGEALRRIGEGAAMIRSKGE
                     AGTGDVSNATTHMRAIGGEIRRLTSLSEDELFVAAKELQAPYELVAEVARAGKLPVTL
                     FTAGGIATPADAAMMMQLGAEGVFVGSGIFKSGSPEHRAAAIVKATTFYDDPDVLAKV
                     SRGLGEAMVGINVEQIAQPHRLAQRGW"
     gene            3306..>4042
                     /locus_tag="VFI55_02315"
     CDS             3306..>4042
                     /locus_tag="VFI55_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPE family protein"
                     /protein_id="HET7072903.1"
                     /translation="MNSARIFAGAGRGPMLAAAAAWDGVAEELHAAAGSVAAVVSELA
                     GESWQGQAALAMTQATGPYVAWLSTAAGQAEQAAAQARLATRAFDMALAATVHPTVVA
                     ANRSELASLVASNLLGLNAPAIAAAEAEYEQMWAQDVAAMSGYHLGASAATQLAPWAQ
                     LLQSLQAELSSAYAASGLPEISVDLATGTFNVGSGNVGVDNIGSGNIGNGNIGVANLG
                     NGNVGIDNIGNANFGIGNTGNRLIGFGR"
BASE COUNT          616 a         1377 c         1361 g          688 t
ORIGIN      
        1 tcgccgaggt cgttgacgtc ctcggcgccg gcgtcgagca ccgcggtcag cacgtcgtcc
       61 tcggtcaggc cgttcttctc cagcgctacg acgcccttgc gggagaacag gtaggacacc
      121 gaacccgggt ccgccatcgc gccgccgttg cgggtcatcg ccacccggac ctcgctggcc
      181 gcacggttgc ggttgtcggt caggcactcg atcagcaccg ccacgccgtt gggcgcgtag
      241 ccctcgtaca tgatcgtctg ccagtcggcg ccgccggctt cctcgccggc gccgcgcttc
      301 cgcgcgcgct cgatgttgtc gttgggcacc gaggtcttct tcgccttctg aatggcgtca
      361 tacagcgtcg gattgcccgc cgggtcacca ccaccggtac gggcggcgac ctcgatgttt
      421 ttgatcagcc gggcgaactc cttgcctcgg cgcgcgtctt tgacggcctt ctggtgcttc
      481 gtggtggccc acttggaatg gccgctcatc gatgttccat atctcttctg ttgctcttct
      541 gttgctcgaa agttcgccag acgagtctac gtgcatggtc ggcgccgatt gcccggccgc
      601 tcccccggcg cctcagcatc cccgcgagtc acgcggactc ggggccaccg gctgtgccca
      661 cccgatgggg cgtcagagtt ttccgttgac gatgtcgacg aacatgtggt ggatgcggcg
      721 gtcgccggtc atctccgggt gaaacgccgt cgccagcgcc gcgccctgcc gcaccgcgac
      781 gatgtgcccc gccgcccggg ccagcacctg cacgccgtcg cccgcccgct ccacccaggg
      841 tgcgcggatg aacaccgcac gcaccggtcc gtccaggccg gcgaactcga tgtcgccctc
      901 gaaggagtcg acctgccgcc caaaagcatt gcgccgcacc gtcatatcga tcgcgcgcag
      961 cggcagcgcc tgccggcccc tggcgccggc gtcgaggatc tggctggcca gcatgatcat
     1021 gccggcgcac gcaccgtagg ccggaagccc gtcggccagc cgtccgcgca agggctccag
     1081 cagctcgaag tcgagcagca ggtgggtcat ggtggtggat tcaccgccgg gaatgaccag
     1141 cccgtccacc gcgtccagct cgctgcggcg ccgcaccggc atggactcgg ctccggcttc
     1201 gcgcagcgcc gccaggtgct cacgggtgtc gccctgcagc gccaaaaccc cgatcttggg
     1261 tgtggtcacc gaacgatcat tgcgtctcgg gctggtatcc gcgctcgtag cgggtgaggc
     1321 cctcctgcat caccgcggcg accatcgcgc cggactgcgt gaagatcttg ccctgggtca
     1381 gagcccgacc gccgcaggcc gacggcgacg actggtcgta gagcagccac tcatcggccc
     1441 gaaacccgcg catgaaccac atcgcgtggt cgagcgacgc cacctgcatg tgcttgcgct
     1501 catcgaggtg attgacctgt gccgatccca acagggtgag gtcgctcatg taggccaacg
     1561 cgcagatgtg cagcaccggg tcgtcgggca gcggatcgcg gtggcgaaac cagacctgct
     1621 gctgggaggc cttgccgggc agcagctgga tgcggtcccg cggcacgatg cggacgtccc
     1681 actcctcgaa ctgacgaaac ccggagttcg cgtacaccca gaccgagtcc aacccgggca
     1741 ggccgtcggg cggcggcgcc gccggcatgg cgtcctggtg gctgatgccc tgctgaggcg
     1801 tctggaacga cgcgcccatg ctgaaaatga tttccccgtg ctggatcgcg ttgacgcggc
     1861 gggtggtgaa cgatccgccg tcgcgggtgc gctcgacgag aaaaacggtg ggctccttgg
     1921 cgtctccggg ccggagaaag taaccgtgca gcgagtgcac gtgatagcgc gggtcgacgg
     1981 tgcgcacggc ggacaccagc gactggccgg ccacgtgacc gccgaaggtg cgctgcagga
     2041 aacccgattc cgggctgaag acgctacctc ggtagatgtt gacctcgagc tgctcgagat
     2101 cgaggatttt ttggatcgac acaggatgtt tttaccagcc gcgctgggcc aggcgatggg
     2161 gctgcgcgat ctgctccacg ttgatcccca ccatcgcctc gcccaagccc cgagacacct
     2221 tggccagcac atcggggtcg tcgtagaacg tggtggcctt cacgatcgcg gcggcgcggt
     2281 gttccgggga gcccgacttg aagatgcccg atcccacgaa caccccttcg gcgccgagct
     2341 gcatcatcat cgccgcgtcg gcgggggtgg cgatgccgcc cgcggtgaac agcgtgaccg
     2401 gcagcttgcc cgcccgggcg acttcggcga caagctcgta gggtgcctgc aattcctttg
     2461 ccgccacgaa caattcgtct tcggacaacg acgtcagccg gcggatttcg ccgccgatgg
     2521 cccgcatgtg cgtggtggcg ttggagacgt caccggtgcc ggcttcgccc ttggagcgga
     2581 tcatggccgc gccctcgccg atgcgccgca gcgcctcgcc gaggttggtc gccccgcaca
     2641 cgaacggcac ggtgaacttc cacttgtcga tgtggtgggt gtagtcggcg ggagtcagca
     2701 cctccgactc gtcgacgtag tccacgccga ggctctgcag gatctgggct tccacgaaat
     2761 gcccgatgcg ggccttggcc atcaccggga tggtcaccgc ggcgatgatg ccctcgatca
     2821 tgtcggggtc actcattcgc gacaccccgc cctgcgcgcg gatatcggcc ggaacccgtt
     2881 ccagcgccat caccgcgacg gcgccggcgc cttcggcgat gcgggcctgc tccggggtga
     2941 cgacgtccat gatgacgccg cccttgagca tctcggccat gccgcgtttg acccgcgccg
     3001 ttccggtctg gttgcgctgg ctcgactgtg cggcgctgtc cactggcctg tccactaccc
     3061 tcctttgatc tccgtcaccc cgtttaattc gcgcaccagt ctagtggtgt ccgacgaccg
     3121 ggtttctcca gcatgcggga caactcgggc ggaaacccca agatccgggc ggacatgtca
     3181 tagggttttc gactaccgga gtgccctttg catgcggcgt ctctaggtgg tggctgggtg
     3241 gtggctaccg agaaaggcca agtgacatca gcgtagcgac gaattttgcc gtgttgccgc
     3301 cggaaatgaa ttcggcgcgc atattcgccg gtgcgggccg gggcccgatg ctggcggcgg
     3361 cggcggcctg ggacggggtg gccgaggagt tgcacgccgc cgcgggctcg gttgcggcgg
     3421 tggtttcgga gctggccggc gagtcctggc agggccaggc ggcgctggcc atgacacagg
     3481 cgacgggccc gtacgtggcg tggctgagca cggccgcggg gcaggccgag caggcggccg
     3541 cccaggcccg gttggccacg cgcgccttcg acatggcgtt ggccgccacc gtgcacccga
     3601 cggtggtggc cgcgaaccgt agcgagctgg cctcgttggt cgcatccaac ctgctcggcc
     3661 tgaacgctcc ggcgatcgcg gccgccgagg ccgaatacga gcagatgtgg gcccaggacg
     3721 ttgccgcgat gtctggctat cacctcggcg cgtcggcggc cacgcaattg gccccgtggg
     3781 cgcagttgct gcaaagcctg caggccgagc tgagcagcgc ctacgccgcc tcggggctcc
     3841 cagaaatcag cgtggatttg gctaccggca ccttcaatgt gggtagcgga aacgtcggcg
     3901 tcgacaacat cggcagcgga aacatcggca acggaaacat cggcgtcgcg aacctgggca
     3961 acggcaacgt cggcatcgac aacatcggca acgcgaactt cggcatcggg aacaccggca
     4021 acagacttat cggcttcggg cg
//
LOCUS       DASSGQ010000054        11852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1279965,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000054 DASSGQ010000000
VERSION     DASSGQ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11852
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279965"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..2451)
                     /locus_tag="VFI55_02320"
     CDS             complement(124..2451)
                     /locus_tag="VFI55_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7072904.1"
                     /translation="MAGFGSDLLAVALAGTAAGEHPLRHVAELPARSGRPHDWPQWAE
                     PDVVGAFAERGISAPWSHQFEAAELAHTGRHVVVSTGTASGKSLAYQLPVLNALATDP
                     RARVLYLSPTKALGHDQLRAAYALTAAIPRLHDVAPTAYDGDSPAEVRRFARERSRWL
                     FSNPDMIHLSILRNHARWAVLLRGLRYVVVDECHYYRGVFGSNVAMVLRRLLRLCARY
                     SARPDLAPTVIFASATTDSPGATAAALIGLPVEEVTEDGSPQGARTVALWEPALRTDL
                     TGENGAPVRRSAGAEAARVMADLIAEGAQTLTFVRSRRAAELTALGAQARLDDIAPEL
                     SPTVASYRAGYLAEDRAGLERALAEGRLRGLATTNALELGVDIAGLDAVVLAGFPGTV
                     ASFWQQAGRSGRRGQGALVVLIARDDPLDTYLVHNPAALLEKPVERVVIDPVNPYILG
                     PQLLCAATELPLDEIEVREFGATEVAQGLIDDGLLRRRNGKYFPAPGIQPHGAVDIRG
                     SAGGQIVIVEADTGRLLGSVGVGQAPATVHPGAVYLHRGDTYVVDSLDFEDGIAFVHA
                     EDPGYATFARELTDIVVTGSGERLRFGPVTLGLVPVTVTHQVVGYLRRRLSGEVLDFV
                     EVDMPQQTLATAAAMYTITPDALLHNGIEVPRIPGSLHAAEHAAIGLLPLVASCDRGD
                     IGGMSTAIGPDGLPSVFVYDGYPGGAGFAERGFRQARTWLGATAAAIDACECPSGCPS
                     CVQSPKCGNGNDPLDKAGAVLVLRLVLAELGGKSR"
     gene            2712..2915
                     /gene="cspA"
                     /locus_tag="VFI55_02325"
     CDS             2712..2915
                     /gene="cspA"
                     /locus_tag="VFI55_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HET7072905.1"
                     /translation="MPQGTVKWFNAEKGFGFIAPEDGSADVFVHYTEIQGSGFRTLEE
                     NQKVEFEIGHSPKGPQATGVRSL"
     gene            3097..3687
                     /locus_tag="VFI55_02330"
     CDS             3097..3687
                     /locus_tag="VFI55_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072906.1"
                     /translation="MSQLSFFTAESVPPAVADLSGVLAASGQVVLVGTGDDKGARLSV
                     VVDRLWRASALAEMIREAGLVPEITRTDEDTPLVRTAVEPSLTTIAAEWTRGAVKTVP
                     PRWLPGPRELRVWTLAAGSPEGDHYLLGLDPHAPDTHSPLASALMRVGIAPTLIGTRG
                     GHPALRISGRRRLSRLVENVGEPPDAAEALSLWPRV"
     gene            3953..6772
                     /gene="topA"
                     /locus_tag="VFI55_02335"
     CDS             3953..6772
                     /gene="topA"
                     /locus_tag="VFI55_02335"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET7072907.1"
                     /translation="MKDPRSPSSGGNGNRRRLVIVESPTKARKLAGYLGSRYIVESSR
                     GHIRDLPRAAADVPAKFKSEPWARLGVNVDADFEPLYIISPEKKSTVSELKGLLKDVD
                     ELYLATDGDREGEAIAWHLLQTLKPSVPVKRMVFHEITEPAILEAAENPRDLDIDLVD
                     AQETRRILDRLYGYEVSPVLWKKVAPKLSAGRVQSVATRIIVQRERDRMAFRSASYWD
                     IVAQLDASVSDPEAQPPTFSARLTNVDGQRVATGRDFDSLGQLRKSATSSSEVVILDE
                     ASATELATGLRGAQLRVASVEEKPYTRRPYPPFMTSTLQQEAGRKLRFSSERTMSIAQ
                     RLYENGYITYMRTDSTTLSESAITAARNQARQLYGEEYVSPSPRQYTRKVKNAQEAHE
                     AIRPAGETFATPDAVRRELDGDEFRLYELIWQRTVASQMADARGTTLSLRMAGTAGGA
                     AGERQVVFSASGRTITFAGFLKAYVETVDELAGGEADDTERRLPHLTEGQRLEALELT
                     PDGHATNPPARYTEASLVKALEELGIGRPSTYSSIIKTIQDRGYVDKKGSALVPSWVA
                     FAVTGLLEQHFGRLVDYDFTAAMEDELDEIASGNEQRTNWLNHFYFGGDHGVPGSVAR
                     SGGLKKLVGVNLEGIDAREVNSINLFDDEQGRPVYVRVGKNGPYLERVVVGDDGEPTP
                     QRANLNDSLTPDELTLEVAETLFSTPQEGRVLGVDPATGHEIVAKDGRYGPYVTEILP
                     EPEPPADAGAEPAKKGKKAAAKGPKARTGSLLRDMDLQTVTLEDALKLLSLPRVVGVD
                     PASGEEITAQNGRYGPYLKRGSDSRSLATEDQMFDITLDEALKIYAEPKRRGRQSASA
                     PPLRELGTDPASGKPMVIKDGRFGPYVTDGETNASLRKGDDVLSITDERAAELLADRR
                     ARGPAKRPAKKATRKAPAKKAVKRT"
     gene            complement(6769..8430)
                     /locus_tag="VFI55_02340"
     CDS             complement(6769..8430)
                     /locus_tag="VFI55_02340"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7072908.1"
                     /translation="MLVVSHQLRPARYGGLVATTAALPGRISAFVRWVARTPWPLFSL
                     SMLQADIIGGLFVLGFLRYGLPPQDRIQLQDLPPVNLAVFVSVVITLFLVGFAVNLRM
                     LVPVFRWQRRDNLLADSDPAATELARSRALRMPFYRTVSSMVAWCIGGVVFIIASWSV
                     AKSAAPVVAVATALGATATSIIGYLQSERVLRPVAVAALRSGVPENVKAPGVILRLML
                     TWMLSTAVPLLAIVLAVAADKASLLHASPEKLFNPILLLALAALGIGLISTLLVAMSI
                     ADPLRQLRWALSEVQRGNYNAHMQIYDASELGLLQAGFNDMVRDLSERQRLRDLFGRY
                     VGEDVARRALERGTELGGQERDVAVLFVDLVGSTQLAATRPPAEVVHLLNEFFRVVVD
                     TVGRHGGFVNKFQGDAALAIFGAPIEHPDASGGALGAARELHDELLPVLGSAEFGIGV
                     SSGRAIAGHIGAQARFEYTVIGDPVNEAARLTELAKLEAGHVLASAIAVSGALDAEAL
                     CWDVGEVVELRGRTAPTQLARPLNLAAPGEVLSDEVSSEVGSQLS"
     gene            8539..9756
                     /locus_tag="VFI55_02345"
     CDS             8539..9756
                     /locus_tag="VFI55_02345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET7072909.1"
                     /translation="MSGVFTRLVGQEAVEAELLAAARAARGDSAHSEAGSGSMTHAWL
                     ITGPPGSGRSIAAVCFAAALQCTSSPEDGGPGCGRCRACTTTMAGTHADVRRIIPEGL
                     SIGVGEMRAIVQIASRRPTTGHRQIVVIEDADRLTEGAANALLKVVEEPPPSTVFLLC
                     APSVDPEDIAVTLRSRCRHVALVTPSTEAIARVLVDSDGLAADTANWAASVSGGHVGR
                     ARRLATDPEARQRRERALGLVRDAATPSRAYAAAEELVGAAETEAVVLTADRAEAETE
                     ELRTALGAGGTGKGTAGTLRGVTGAIRDLERRQKSRVTRASRDALDRALIDLATYFRD
                     ALVVSANAGAVRANHPDMADRVAALAGHASPERLLRCIEAVLECREALAINVKPKFAV
                     DAMVATIGQELRD"
     gene            9819..9894
                     /locus_tag="VFI55_02350"
     tRNA            9819..9894
                     /locus_tag="VFI55_02350"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9852..9854,aa:Thr,seq:cgt)
     gene            complement(9970..11733)
                     /locus_tag="VFI55_02355"
     CDS             complement(9970..11733)
                     /locus_tag="VFI55_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072910.1"
                     /translation="MGSEFSAAALITLHLGIRAVLAFGGYFYWDDLILIGKAGTQGLL
                     SPSYLFDDHDGHLMPAAFLVAGAIIRLAPLDWTGPAISLLVLQLLASLALLRALCVIL
                     GRRWVLLIPLTFALFTPLAVPGFAWWAAALNSLPMLAALAWVCADAILLVRTGNRRYA
                     ATGVLVYLGGLLFFEKAAVIPFVAFAVAALLCHVAGDGSAVRTVWRAGMRLWIASLTL
                     TAAWIALYLAAVNQRRWSWDLSMTWDLLWRSITHGIVPGLAGGPWRWDRWAPASPWAA
                     PPAAVMALGWLVLTGVFVLSLIRKERIGPVWLTAVGYAVACQVPIYLMRSSKQTALEL
                     AQTLRYLPDLVVVLALLAAVALCAPNRESSRRLDASRQRTAVTTGLAAAFAASSLYST
                     ATFLTSWQDNPTKSYLQNARAGLATAHAASDAPLLDQEVDPLVLQRVAAPENLASHMF
                     ALLRNRPEFATATTHLRMLNSSGRLIDARVTWIRTIAPGPTPRCGYFAQPDKPARLIV
                     DGPLLPADWTVELNYLANTDGSMTLSLTEGPDVKVPVHPGLNRVFARLPGAGDVITAR
                     ADTSALALCIASGPVGFLAPA"
     gene            11708..11852
                     /locus_tag="VFI55_02360"
                     /pseudo
     CDS             11708..11852
                     /locus_tag="VFI55_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218152.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2037 a         4028 c         4077 g         1710 t
ORIGIN      
        1 cgcgctgcca cctaagcccc gacggaagtt gcggtcgccc aatgccttgc tacgtcaacg
       61 caatccgtgg tggtggccag actacgacgc aagccaccac ccagcttcgc ctaccggcct
      121 tgctcagcgt gatttgccac ccaactccgc aagcaccaac cgaagcacca gcacagcccc
      181 cgccttgtcc agcgggtcgt tgccgttgcc gcacttgggc gactgcacac acgacgggca
      241 tccgctgggg cattcgcatg cgtcgatggc cgcggccgtc gcacccaacc aggtgcgagc
      301 ctggcggaag ccgcgttcgg cgaaacccgc gccgcccggg tagccgtcgt agacgaagac
      361 actgggcagc ccatcggggc cgatcgcggt ggacatgccg ccgatgtcgc cgcggtcaca
      421 gctcgccacc aggggcagca gaccgatggc agcatgttcg gcggcatgca acgacccggg
      481 gatccgtggc acttcaatac cattgtgcag caaagcatct ggtgtgatcg tgtacatggc
      541 cgcggcggtg gccagcgtct gttgcggcat gtcgacctcg acgaagtcga gcacctcacc
      601 ggacagccgg cgacgcaggt aacccaccac ctgatgggtg actgtgaccg ggaccaggcc
      661 cagggtgacg ggcccgaatc gcaagcgttc accgctgccg gtgacgacga tgtcggtgag
      721 ctcgcgcgcg aacgtcgcgt agccggggtc ctcggcgtgc acgaaggcga tcccgtcctc
      781 gaagtccagc gagtccacga cgtacgtgtc gccccgatgc aggtagacgg ctccggggtg
      841 gaccgtggcc ggggcctgac cgacgccgac gctgccgagc agccggcctg tgtccgcctc
      901 cacgatgacg atctggccgc ccgccgagcc gcgaatgtcc acggcaccgt gcggttgtat
      961 gccgggcgcc ggaaagtact tgccgttgcg ccgccgcagc aacccgtcgt cgatgaggcc
     1021 ttgggccacc tcggtggcac cgaactcccg aacctcgatc tcgtcgagcg gcagttcggt
     1081 tgcggcgcaa agcagttggg ggccgaggat gtatgggttg acggggtcga tcacgacccg
     1141 ctcgacgggc ttttccagca gcgcggcggg gttgtgcacc agataggtgt ccagcggatc
     1201 gtcgcgggcg atcaagacga ccagcgcgcc ctggccgcgc cggcccgacc ggccggcctg
     1261 ctgccagaac gacgcgaccg tcccggggaa accggcgagc accaccgcgt ccagcccggc
     1321 gatgtcgacg cccagctcca acgcgttggt ggtggccagg ccgcgcagcc ttccctcggc
     1381 cagggcgcgc tccagcccgg cgcggtcctc ggcgagatac cccgcccgat acgacgccac
     1441 cgtcggggac agctccgggg cgatgtcgtc cagccgcgcc tgggcgccca gcgcggtcag
     1501 ttccgcggcg cgcctcgacc ggacgaaggt cagggtctgc gctccctcgg cgatcaggtc
     1561 ggccatcacg cgcgccgcct cggcgccggc ggatcgccgc accggcgccc cgttctcacc
     1621 ggtcaggtcg gtccgcagcg ccggctccca caaagccacc gtccgggccc cctgcggtga
     1681 gccgtcttcg gtcacctcct cgaccggaag accgatgagc gcggcggcgg ttgcgcccgg
     1741 cgaatccgtc gtcgcgctgg cgaagatcac cgtcggcgcg agatcaggcc gagccgagta
     1801 acgcgcgcac agccgcaaca gccggcgcag caccatcgcc acgttcgagc cgaaaacccc
     1861 acggtagtaa tggcattcgt cgacgaccac gtagcgcagg ccacgcaaca aaaccgccca
     1921 gcgggcatgg ttgcgcagga tcgacagatg aatcatgtcc gggttggaga acaaccagcg
     1981 ggagcgttcc cgggcgaagc ggcgcacctc ggcggggctg tcgccgtcgt aggcggtggg
     2041 cgcgacgtca tgcagccgtg gaatggccgc cgtcagcgcg tatgcggcgc gcaactgatc
     2101 gtggccgagt gctttggtcg gcgacaggta aagcacccgg gccctcgggt cggtcgccag
     2161 cgcgttgagg accggaagct gataggccaa tgacttgccc gacgcggtgc cggtgctgac
     2221 cacgacgtga cgcccggtgt gcgccagctc ggcggcctcg aactggtgtg accacggggc
     2281 gctgataccg cgttcggcaa atgcgccaac cacgtccggc tccgcccact gcggccaatc
     2341 gtgtggccgg ccgctgcgcg ccggcaactc ggcgacatgg cgcaacggat gctcgcccgc
     2401 agcggtaccg gcgagcgcga cggcaagcag gtcgctgccg aaacccgcca cctcacaccc
     2461 tccaagaatc cggcccagaa tccgtctgtg acacaagtct gtcgccgacg cgccgaacgt
     2521 gattgactaa gtgcggtcgt agcttctgtg ttcgtgtcaa actttcgcag gatcgacgtt
     2581 gcggccgcgg ttcctgcgag gttatcggtc gggtgaagtt ccggcgaatc cgcgaggtat
     2641 ggcggtctca gctggcccgg ggcatcgaaa ggcgctgccc cgcacccata aaaagaagga
     2701 aagatcgaga aatgccacag ggaactgtga agtggttcaa cgcggagaag gggtttggct
     2761 tcatcgcccc cgaagatggt tccgcggatg tttttgtcca ctacacggag atccagggtt
     2821 cgggcttccg cacccttgaa gaaaaccaga aggtcgagtt cgagatcggc cacagcccta
     2881 agggccccca ggccactgga gtccgctcac tgtagagaag agcagattcc gacgtaaacc
     2941 ccccgtgcgg tcccgcacag cggatctcgc cggggggttt tcgtttttgt ccagaggacc
     3001 ctgcgggccg gccacccgcc gggcccacct cgcccccaag ccgccgcgcc ggatggttca
     3061 aggcgtggac ggacgacctg gcctactgtc ggtggcgtga gccagctttc cttcttcaca
     3121 gcggagtcgg tgccgcccgc ggtcgccgat ctctccgggg tgctggcggc ttctggccag
     3181 gtcgtactgg tcggaactgg agacgacaag ggtgctcggc tttcggtggt ggtggatcgg
     3241 ctctggcgcg cctcggcgct ggccgagatg atccgcgagg cgggtttggt gcccgagatc
     3301 acccgcaccg acgaagacac cccgttggta cgcacggcgg tggagccttc gctgaccaca
     3361 atcgccgccg aatggacgcg aggcgcggtc aagaccgtgc cgccacgctg gctgcccggg
     3421 ccgcgtgagc tgcgggtgtg gacgctggcc gccggcagcc ccgaaggcga ccattacctg
     3481 ctgggtctgg acccacacgc ccccgacacc cattccccac tggcgtcggc gctgatgcgg
     3541 gttgggatcg caccgaccct gatcggcacc cgcggcggtc acccggcgct gcggatcagc
     3601 ggccgtcgca ggctatcgcg cctggtagag aacgtggggg aacccccgga cgcggccgag
     3661 gcgttgtcgc tctggccccg cgtttagccg tgcgacgata cgggccgtga gcggcccgag
     3721 gaggagtgcg gcagtcaggt ttagccgtgc ggcaggacct gcgatttggg tagccggttt
     3781 gcgaaggccc gcccgagggt gcgaaattgt caggtgccct aaagagaagg aacgctggcg
     3841 tacccgaatt tcaccggaat ttcaggcgct agcctgtcat tcttcctgca gggcggtctc
     3901 gtagggggcc gagataagag atggagcgta agcgcagttg cctgagccga agctgaagga
     3961 ccctagaagc ccgagcagcg gcggaaatgg gaaccgtagg cgactcgtga ttgtcgagtc
     4021 gccgaccaag gcgcggaaac tggcgggcta cctgggctcc aggtacatcg tcgaatcctc
     4081 gcgcggccat attcgcgacc tgccccgggc ggcggccgac gtgcccgcga agttcaagtc
     4141 ggagccctgg gccaggctcg gggtcaacgt cgatgccgac tttgagccgc tgtacatcat
     4201 cagcccggag aagaaaagca ccgttagcga actcaagggc ctgctcaagg acgtcgacga
     4261 gctctatctg gccacggatg gtgaccgcga gggcgaagcc atcgcgtggc acctgttgca
     4321 aactctcaaa cccagcgtcc cggtcaagcg gatggtcttt cacgagatca ccgaaccggc
     4381 catcctcgaa gccgcggaaa acccccgcga cctggacatc gacctggtcg acgcgcagga
     4441 aacccggcgc atcctggaca ggctgtacgg ctacgaggtc agcccggtgc tgtggaagaa
     4501 ggtcgcgccc aagctgtcgg cgggccgggt ccagtcggtg gccacccgga tcatcgtgca
     4561 gcgcgaacgc gaccgcatgg cgttccgcag cgcgtcctac tgggacatcg ttgcccagct
     4621 ggacgcgagc gtgtccgacc ccgaagccca gccgcccacc ttcagcgccc gactgacgaa
     4681 cgtcgacggc cagcgcgtgg ccaccggccg cgatttcgac tcgctgggcc agctccgcaa
     4741 gagcgccacc tcgtcgtcgg aggtcgtaat cctggacgag gcgagcgcga ctgagctggc
     4801 caccgggtta cgcggcgcgc agttgcgcgt ggcctcggtg gaggaaaagc cctacacccg
     4861 tcgtccctat ccgccgttca tgacgtcgac gctgcagcag gaagccggcc gtaagttgcg
     4921 gttctcctcc gagcgcacga tgagcatcgc ccagcggctt tatgagaacg gctacatcac
     4981 ttatatgcgg accgactcca cgacgctgtc ggagtcggcg atcaccgcag cccgaaatca
     5041 ggcgcgccag ctctacggcg aagagtacgt ctcaccgtcg ccgcggcagt acacccgcaa
     5101 ggtgaagaac gcccaggagg cgcacgaggc gatccggccc gccggcgaga cgttcgccac
     5161 cccggatgcg gtgcgccgcg agctcgacgg cgacgaattc cggctctacg agctgatctg
     5221 gcagcgcacg gtggcctcgc agatggccga cgcgcgcggc accacgctga gcctgcggat
     5281 ggccggtacg gcgggcgggg ccgccggaga aaggcaggtg gtgttctcgg ccagcggtcg
     5341 gaccatcacg ttcgccgggt tcctcaaggc ctacgtggaa accgtggacg agttggccgg
     5401 cggcgaggcc gacgacaccg agcgccggct gcctcatctg accgaggggc agcggctgga
     5461 ggcccttgag ctcacgcccg acggccatgc caccaacccc cctgcgcggt acaccgaggc
     5521 gtcgctggtc aaggcgctcg aggagttggg catcggccgc ccgtcgacgt actcgtcgat
     5581 catcaagacc attcaggacc gcggctacgt cgacaagaag ggcagcgcgc tggtgccctc
     5641 gtgggtcgcg ttcgcggtca ccggactgct ggagcagcat ttcggccggc tcgtcgacta
     5701 cgacttcacc gcggcgatgg aagacgagct cgacgagatc gcgtcaggca acgagcaacg
     5761 caccaactgg ttgaaccact tctacttcgg cggggaccac ggggtgcccg gttcggtggc
     5821 ccgctccggt ggcctgaaga agttggtcgg tgtcaacctc gagggcatcg atgcccgaga
     5881 agtcaactcc atcaatctgt ttgacgacga gcagggccgc cctgtctatg tccgggtggg
     5941 caagaacggg ccctacctgg aacgcgtggt ggtcggcgac gacggtgagc ccacgccgca
     6001 gcgggccaac ctcaatgact cgctcacccc tgacgagctg acgctcgaag tggccgaaac
     6061 ccttttctcc acaccgcaag agggtcgcgt cttgggtgtg gatccggcga cgggtcacga
     6121 aatcgtcgcc aaggacggcc ggtatgggcc gtacgtgacc gagatcctcc cggagcctga
     6181 gccgcccgcc gacgcgggcg ccgagcccgc caagaagggt aagaaggctg ctgccaaggg
     6241 ccccaaggcc cggactggtt cgctgctgcg cgacatggac ctgcagacgg tcaccctcga
     6301 ggacgcgctg aagctgctgt cgctgccccg ggtggtcggc gtggatcccg cctccggtga
     6361 ggagatcacc gcgcagaatg ggcgctatgg cccatacctg aagcgcggca gcgattctcg
     6421 ttcgctggcg accgaggatc agatgttcga catcactctg gacgaggcgc tgaaaatcta
     6481 cgcggagccc aaacgccgtg gcaggcagag cgcttcggca ccgccgttgc gcgagctggg
     6541 gaccgatccg gcgtccggta agccaatggt catcaaggac ggccgatttg ggccctacgt
     6601 caccgacggc gagaccaacg cgagcctgcg caagggcgac gacgtgctgt cgatcaccga
     6661 cgagcgtgcc gccgaactgc tggccgaccg gcgagcccgg ggtccggcca agcggcccgc
     6721 aaagaaggcc actcgcaagg cgccggcgaa gaaagccgtc aagcgcacct agctgagctg
     6781 gctacccacc tcgctggaaa cctcgtcgct gagcacttcc cccggagcgg ccagattcag
     6841 cggccgggcc agctgggtgg gcgccgtgcg cccgcgcagc tcgacgacct caccgacatc
     6901 ccagcacagg gcttcggcgt ccagcgcgcc gctgaccgcg atcgccgagg cgaggacatg
     6961 gccggcctcg agcttggcca gctcggtgag ccgcgcggcc tcgttgaccg gatcgccgat
     7021 caccgtgtac tcgaagcggg cctgcgcacc gatgtggccc gcgatggccc tgccggacga
     7081 caccccgatc ccgaactccg cggaacccag cactgggagg agctcgtcgt gcagctcacg
     7141 ggcggcgccc agtgcgccac cggacgcgtc ggggtgttcg atcggcgcgc cgaagatcgc
     7201 cagcgcggcg tcgccctgga acttgttgac gaagccaccg tgccggccga cggtgtcgac
     7261 cacgacccgg aagaactcgt tgagcaggtg gaccacctcg gcgggcggtc gtgtcgcggc
     7321 cagctgggtg gagccgacca ggtccacgaa caggaccgcg acgtcgcgct cctggccacc
     7381 cagctcggtg ccgcgctcca gcgcccggcg agccacgtct tcgcccacgt agcgaccgaa
     7441 caggtcgcgc agccgctgcc gttcggacag atcacgcacc atgtcgttga agccggcctg
     7501 cagcaggccc agttcgctgg cgtcatagat ctgcatgtgc gcgttgtagt tgccgcgttg
     7561 cacctcggac agcgcccagc gcagctggcg cagcgggtcg gcgatcgaca tggcgaccag
     7621 cagggtgctg atcagcccga tcccgagtgc cgccagtgcc agcagcagga tggggttgaa
     7681 cagcttttcg ggtgacgcat gcagcaggga ggccttgtcg gccgccacgg ccagcacgat
     7741 cgccagcagc ggcacggcgg tagacagcat ccaagtcagc atcagccgca ggatgacgcc
     7801 gggggccttg acgttctccg gcacgccgct gcgcagtgcc gcgaccgcca ccggccgcaa
     7861 cacccgctcg gactgcaggt agccgatgat cgaggtggcg gtggcaccca gtgccgtggc
     7921 gacggccacc acgggcgccg cggacttggc caccgaccag ctggcgatga tgaacaccac
     7981 gccgccgatg caccacgcga ccatgctgct caccgtgcgg tagaacggca tgcgcaacgc
     8041 gcggctgcgg gccagttcgg tcgcggcggg atcggagtcg gccagcaggt tgtcgcgtcg
     8101 ctgccatcgg aaaaccggca ccagcattct caggttcacc gcgaacccga ccaggaacag
     8161 cgtgatgacc acgctgacga agacggccag gttgaccggt ggcaggtcct gcaactggat
     8221 gcggtcctgg ggcggcaagc cgtagcgcag aaagcccagc acgaagaggc cgccgatgat
     8281 gtcggcctgc agcatgctca gcgagaacaa cggccacggg gtacgcgcca cccaacggac
     8341 gaatgcgctg atccgccccg gtagtgctgc cgtggttgcc accaatccac cgtatcgggc
     8401 ggggcggagt tggtgggaaa ctacaagcat ctaggcaagt cgccccggag ctgccgaaac
     8461 acccggcaat gtcacggaac tgtcgcgatt cggccaccca tcgttatctg tcgcccggtg
     8521 tcactactgt tgccggtgat gtccggggtg tttacgcggc tggtaggcca agaagcggtg
     8581 gaggccgagc tgctcgccgc cgcccgcgcg gcccgtggtg attcggctca cagcgaggcc
     8641 ggcagcgggt ctatgacaca tgcgtggctg atcaccgggc caccgggttc cggtcgctcg
     8701 atcgcggcgg tgtgcttcgc ggccgcgctg cagtgcacct cttctccgga agacgggggc
     8761 cccggctgcg ggcgctgccg ggcgtgcacg acgacgatgg ccggcacgca cgccgacgtg
     8821 cgccgcatca ttcccgaggg cttgtcgatc ggcgtgggcg agatgcgggc catcgtgcag
     8881 atcgcgtcgc gccgtccgac caccggacac cggcagatcg tggtgatcga ggacgccgac
     8941 cggctgaccg agggtgccgc caacgcgctg ctcaaggtgg tcgaggaacc accgccgtcg
     9001 accgtgttcc tgctgtgcgc gccgtcggtg gatcccgagg acatcgcggt cacgttgcgg
     9061 tcccggtgcc ggcatgtcgc cctggtgacg ccgtcgaccg aggcgatcgc gcgggtgctg
     9121 gtcgacagcg acggcctggc cgccgacaca gcgaactggg cggcgtcggt gagcggtggc
     9181 catgtggggc gggcgcgccg gttggccacc gatccggaag cccggcagcg gcgggagcgg
     9241 gcgctgggac tggtgcgcga tgccgcgacc ccgtcacggg cctatgccgc cgccgaggaa
     9301 ctggtgggtg cggccgagac cgaggcggtg gtgttgaccg ccgaccgcgc cgaggccgag
     9361 acggaagagc tgcggacggc cctcggggcc ggcggcacgg gtaagggcac tgcgggcacg
     9421 ttgcggggcg tcacgggggc gatcagggac ctcgagcgcc ggcagaagtc gcgtgtgacg
     9481 cgggcgtcgc gcgatgccct cgaccgggcc ctgatcgacc tggcgaccta tttccgggac
     9541 gctctggtgg tgtcggcgaa tgccggggcc gttcgggcca accatccgga tatggccgac
     9601 cgggtggcgg cgctggccgg ccatgcctcg ccggagcggc tgctgcgctg catcgaggcg
     9661 gtgctggagt gccgcgaggc gctggcgatc aacgtcaaac cgaaattcgc cgtcgacgcc
     9721 atggtggcca ccatcggtca ggaactgcgg gattgatttt ttggagtttg ggggtgcccg
     9781 cccgcctgcc gtagactcgt gccgcccggc gcgggtgtgc cgccttagct cagtcggtag
     9841 agcgattcac tcgtaatgaa taggtcgggg gttcgattcc cccaggcggc tccatttcct
     9901 ttcgccgagc gtgacgccac ggtcacgttc gggcccgaaa gtgaccgtgg cgtcacgctc
     9961 gaggagcgat caggccgggg cgaggaaacc caccggtccg gatgcgatgc acagcgccag
    10021 cgcgctggtg tcggcccgcg cggtgatgac gtcgccggcg cccggcagcc gggcgaacac
    10081 ccggttgagt cccggatgca ccggaacctt gacgtcgggt ccctccgtga gcgacaacgt
    10141 catcgatccg tcagtgttcg ccaggtaatt gagttcgacg gtccaatcgg cgggcagcaa
    10201 cggcccatcg acgatcaacc gcgccggctt atccggttgc gcgaaatagc cgcaccgagg
    10261 ggtgggcccc ggcgcgatgg tgcggatcca ggtcacgcgc gcgtcgatca gccggccgga
    10321 gctgttcaac atgcgcaagt gcgttgtcgc cgttgcgaat tcgggcctat tccgcaacag
    10381 cgcgaacatg tggctggcca ggttctccgg tgcggcgacc cgctgcagca ccagcggatc
    10441 gacctcttgg tccagcagcg gcgcatccga cgccgcatgc gcggtggcca ggcccgcccg
    10501 cgcgttctgc aggtaggact tggtcgggtt gtcctgccag ctggtcaaaa atgtcgcggt
    10561 cgagtacagg ctgctggccg cgaacgccgc ggccaaaccc gtcgtcaccg cggtgcgctg
    10621 ccgagacgca tccaaccggc gcgacgattc ccggttcggg gcgcacaacg cgacggctgc
    10681 cagcaacgcc agcacgacga cgagatccgg cagataacgc aacgtctgag ccaattccag
    10741 ggcggtctgc ttcgatgacc gcatcagata gatgggcacc tggcaggcga cggcgtagcc
    10801 gacggcggtc agccacaccg gcccgatgcg ttctttgcgg atcagcgaca gcacaaacac
    10861 cccggtcagc accagccagc cgagcgccat caccgccgcc gggggagctg cccacggcga
    10921 tgccggcgcc caccggtccc agcgccacgg cccaccggcc agtccgggca cgatgccgtg
    10981 ggtgatcgag cgccacaaca ggtcccacgt catcgacagg tcccaactcc accgccgctg
    11041 attgaccgcc gccagataca gcgctatcca ggcggcggtc aacgtcagcg acgcgatcca
    11101 cagccgcatg ccggcccgcc acaccgtccg caccgcagac ccgtcacctg ccacgtggca
    11161 cagcaacgca gccacggcga atgcgacgaa cggaatcacc gccgccttct cgaagaacag
    11221 cagcccgccc aggtagacca gcacaccggt cgccgcgtag cgccggttgc ccgtgcggac
    11281 cagcaggatc gcgtcggcgc acacccaggc cagcgccgcc agcatcggca gcgagttcag
    11341 cgccgccgcc caccacgcga agcccggcac cgccaacggt gtgaacagcg cgaacgtcag
    11401 cgggatcagc agcacccagc gccggccgag gatgacgcac agcgcgcgca gcagggccag
    11461 cgaggccagc aactgcagca ccagcaggct gatcgccggc cccgtccagt ccaggggcgc
    11521 cagccggatg atcgcgccgg cgaccaggaa ggcggccggc atcaggtggc cgtcgtggtc
    11581 gtcgaacagg tacgacggcg acaacaggcc ctgggtaccg gccttcccga tgagaatcag
    11641 gtcgtcccag tagaagtagc cgccgaacgc cagcaccgca cgtatcccca gatgcagcgt
    11701 gatcagggcg gcggcgctga attcgctgcc catgctggcg gcgctggcct gggtgtgcgc
    11761 cgacgcgatc ctgctggtcc gcaccggcaa ccagcgctac gcggcgacgg gcgtgctggt
    11821 ctatctcggc gggctgctgt tcttcgagaa gg
//
LOCUS       DASSGQ010000055         3650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1279991,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000055 DASSGQ010000000
VERSION     DASSGQ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279991"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VFI55_02365"
     CDS             <1..379
                     /locus_tag="VFI55_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072911.1"
                     /translation="VVLCGFVLLVVPGLVAFPWLVLATPLVSLERTGVWPALRRSIAL
                     VRGNFWPVAALTTLTFVPASLGDWLSDEISHAHAPLWTELLVEIVTDAVAVAVTAAVV
                     VTMFDALCKIAANPAEEPPAVRS"
     gene            complement(438..1937)
                     /locus_tag="VFI55_02370"
     CDS             complement(438..1937)
                     /locus_tag="VFI55_02370"
                     /inference="COORDINATES: protein motif:HMM:NF020768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0031221 - arabinan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinofuranosidase catalytic domain-containing
                     protein"
                     /protein_id="HET7072912.1"
                     /translation="MTVRARLRHARKALLVLTGAVALAAGTLIAGGAAPAGAATSLPC
                     DIYTAAGTPCVAAHSTTRALYAAYNGPLYQVRRASNGATTNIGTLAAGGYANAAAQDS
                     FCAGTTCTITEIFDQSPQHNNLTIGPAGGAGGADVGANATALPVMAGGHRVYGVFVSP
                     GTGYRDDATTGVATGSQPQGAYMVASGTHVNSGCCFDYGNAETNNRDTGNGDMDAIYF
                     GKLCWFSPCANGGPRVAADLENGLFAGGDGSNTQNTGRSSAYVTAMTKNNGTSTYAIK
                     DGNAQSGGLATRYNGALPSQSGYRPMTKQGAIILGIGGDNSNGSVGSFFEGVMTSGFP
                     SDATENSVQSNIVSVGYTKSVTFPVSGNTYRLTNLAAGKVMDAINCGTANGTRIDVWQ
                     ALGNTCQQWRFTNVGPNKWNITNVNSGTVLDAVNCGLALGTEVDLWQSLGNLCKQWAV
                     IPAGNSRYELVVQNSGMVLDDKNCGTANGTRVQLWMWLNNTCQLWSIAP"
     gene            complement(2860..3489)
                     /locus_tag="VFI55_02375"
     CDS             complement(2860..3489)
                     /locus_tag="VFI55_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007296341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7072913.1"
                     /translation="MMLTLNHLVTSGTFSLDGGTWDVDNNVWVLGDDAECVVIDAPHD
                     ADAIAAAVASRRLTAILCTHAHDDHIDAAAALAEATGAPIWLHPADRELWDQTYPGRA
                     PDRDLADGQVITVAGADLTVLHTPGHSRGAVCFHLPDQGWLFSGDTLFAGGPGATGRS
                     FSDFGTIIDSIRDRLLVLDPDTVVHTGHGSDTTIGAERPHLQEWIARGH"
BASE COUNT          526 a         1169 c         1270 g          685 t
ORIGIN      
        1 cgtggttctg tgcgggtttg tgctgctcgt cgtgcccggt ctggtggcgt tcccttggct
       61 cgtcctggcc actccgttgg tgagtctgga gcggacgggt gtctggccgg ccctgcgaag
      121 gagcatcgcc ctggtgcgcg ggaacttctg gccggtggcg gcgctgacca cgttgacgtt
      181 cgtcccagcg tctcttggcg actggttgag cgacgagatc tcgcacgccc atgctccgtt
      241 gtggacggag ctgctcgtcg agatcgtgac cgacgcggtc gccgttgcgg taacggcggc
      301 ggtcgtcgtg accatgttcg acgcgctgtg caagatcgcg gcgaacccgg ccgaggagcc
      361 gccggccgtg cgttcatagc gcactcacgg attggtccgg cgacatgtgc tgcccgcggt
      421 ctacgtcggg gaagcagtta gggcgcgatg gaccagagct ggcaggtgtt gttcagccac
      481 atccagagct ggacgcgcgt gccgttggcg gtgccgcagt tcttgtcgtc gagcaccatg
      541 ccgctgttct gcaccaccag ctcgtagcgg ctgttgccgg cggggatgac cgcccactgc
      601 ttgcacaggt tgcccagcga ctgccacagg tcgacctcgg tgccgagggc caggccgcag
      661 ttgaccgcgt cgagcaccgt gccggagttg acgttcgtga tgttccactt gttggggccg
      721 acgttggtga agcgccactg ctggcaggtg ttgcccagtg cctgccagac gtcgatccgc
      781 gtgccgttgg ccgtgccgca gttgatggcg tccatgacct tgccggccgc caggttcgtc
      841 agccggtacg tgttgccgct caccgggaac gtgacgctct tggtgtagcc gacggagacg
      901 atgttggact gcaccgagtt ctcggtggca tcgctcggga agccactggt catgacgccc
      961 tcgaagaacg agccgaccga tccgttgctg ttgtcgccgc cgatgccgag aatgatcgcg
     1021 ccctgcttgg tcatgggccg gtagcccgac tggctcggca gcgcgccgtt gtaccgcgtg
     1081 gccaggccgc cggactgcgc gttgccgtcc ttgatggcgt aggtcgaggt gccgttgttc
     1141 ttcgtcatcg cggtcacgta cgcgctgctg cgaccggtgt tctgcgtgtt cgaaccgtcg
     1201 ccgccggcga acagaccgtt ctccagatcg gccgcgaccc gcgggccacc gttggcacac
     1261 ggcgagaacc agcacagctt gccgaagtag atggcgtcca tgtcaccgtt gccggtgtcc
     1321 cggttgttgg tctcggcgtt gccgtagtcg aagcagcagc cgctgttgac gtgggtgccg
     1381 ctggccacca tgtaggcgcc ctgcggctgg gatccggtcg ccactcccgt ggtggcatcg
     1441 tcgcggtacc cggttcccgg gctcacgaag accccataga ccctgtggcc cccggccatc
     1501 accggcagcg cggtcgcgtt cgcgccgaca tccgccccgc cggccccgcc ggctggcccg
     1561 atcgtgaggt tgttgtgctg cggggactgg tcgaatatct cggtgatggt gcacgtggtg
     1621 ccggcgcaga acgagtcctg cgccgccgca ttggcgtagc cgcccgcggc gagcgtcccg
     1681 atgttcgtgg tggcgccgtt cgacgcacgc ctgacctggt acagcgggcc gttgtaggcc
     1741 gcatacaggg ccctggtggt gctgtgcgcc gccacgcacg gcgtgccagc cgcggtgtag
     1801 atgtcgcacg ggagggacgt cgccgctccg gctggggccg caccgccggc gatcagggtt
     1861 ccggcggcga gtgccaccgc cccggttagg accagcaacg cctttctcgc gtgccggagt
     1921 cgtgcccgga cagtcatcgt cgagcctgcc tttctcatcc gaaggtccat tgctggctgg
     1981 tcccccgtcg ggcaacgcag caggcgacgg gccggaagcg cgcccggatg ttcgggttta
     2041 aggggcgtgt cacctcctga cgcgtacggg ccaggtcatt gcggtccaga cggcgcggcc
     2101 ggcatggcca cggcgagtcg tgaggttagc gctcacaatt cctgcgcaac ggcttcggtg
     2161 accgcaacgg cttcagcgac gaaatgggga ttgccgtcat tgtgagcgct aacaatccgg
     2221 gcgtcaatag gccggcgttt ctgctcgagc ctgtttctcg tgcgccatcc cgcgacctgg
     2281 gaattcacga ggggaacatt gtttagccca ctgccacgta gctagatggc gttcaaacgc
     2341 gctgaaagtc cagcttcatg gcgcctgcag ggtgggtcct tgtcatggtt cccgaagacg
     2401 gggtcggtgt tactggccgg ccagtcggtg acgccgtgat taccctggtc cgcagtgttg
     2461 acgtacagcg aggccttcgg ctgccgcgtg ccgccgacag aaaactgggc ccagcttatt
     2521 tcgtcctcca ggcaggggtt cgtattgttc gggagcccct cgttcacggt cacgatgccg
     2581 cggcgtgccg accatcgcca tcgcggcgga taccgcggtc acggtccggc cgaatgccac
     2641 cggccagccg cttttccgtc gccccagcag ccgcccggat catgcgcttt ttattaagag
     2701 cgagactgaa ttcaacgcgc cgtgtcctcc ccttcgcacc tggcattgcg tctgatatac
     2761 aagacacgcc agcgaggctt gtggtgtacc tgcgaagtaa cgttagaccg cccgcgcggg
     2821 cggcccgcct gcgggaaggc cggccgggcc gcctgccgat cagtggccgc gggcgatcca
     2881 ttcctgcagg tgaggtcgtt cggcgccgat ggtggtatcc gacccgtgac cggtgtggac
     2941 gaccgtgtcc gggtcgagga cgagcagccg gtcacggatc gagtcgatga tcgttccgaa
     3001 gtcggagaaa gaacgcccgg tcgcgcccgg gccgccagcg aacaacgtgt caccgctgaa
     3061 cagccagccc tgatcgggca ggtggaagca gacggcgccg cgtgaatgtc cgggtgtgtg
     3121 cagcaccgtg aggtcggcgc cggccacggt gatcacctgc ccgtcggcca ggtcccggtc
     3181 tggggcccgg ccgggatagg tctgatccca gagctcgcgg tcggcggggt gcagccagat
     3241 cggcgcaccg gtggcctcgg ccagggcggc ggcggcatcg atatggtcgt cgtgggcgtg
     3301 ggtgcacaag atggcggtga gccgccgcga cgcgacggcg gccgcgatcg cgtccgcgtc
     3361 gtgcggggcg tcgatgacca cgcattcggc gtcgtcgccg agcacccaga cgttgttgtc
     3421 cacgtcccag gtacccccgt caagggagaa cgtgccggac gtaacgagat ggttcaaggt
     3481 gagcatcaag ccgatcctag tgccgcatca gagtaccgcg cagttccgca cctcacgtct
     3541 caggcgtacc gcaacactga tgttctctcg tgcctagccg acccggcgct tagctgatgc
     3601 ggtacatgac tgggtcgatg tgccgttggg caggttagag cagtaccaac
//
LOCUS       DASSGQ010000056         9083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_763292,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000056 DASSGQ010000000
VERSION     DASSGQ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9083
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763292"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..846
                     /locus_tag="VFI55_02380"
     CDS             145..846
                     /locus_tag="VFI55_02380"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HET7072914.1"
                     /translation="MSRAALDKDPRDVASMFDGVARRYDLTNTVLSLGQDRFWRRATR
                     SALGVGPGQKVLDLAAGTAVSTVELTKSGAWCVAADFSVGMLAAGAARDVPKVAGDAT
                     RLPFCDGVFDAVTISFGLRNVADQPAALREMLRVTRPGGRLVVCEFSTPTNGLFATAY
                     KEYLMRALPRVAQAVSSNPDAYVYLAESIRAWPDQATLAHRISAAGWSAVRWRNLTGG
                     IVALHAGYKPLRQGQ"
     gene            complement(854..1204)
                     /locus_tag="VFI55_02385"
     CDS             complement(854..1204)
                     /locus_tag="VFI55_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HET7072915.1"
                     /translation="MKGTSLAASVAVAAPILAGAIALAAPAQADDYDAPFNAQLHTYG
                     IYGPQDYNAWLGKITCERLSRGVDGDAYKSATFIQRNLPLHTTEGQSFQFLGAAIDHY
                     CPDQVGVLQRAGTR"
     gene            complement(1233..1919)
                     /locus_tag="VFI55_02390"
     CDS             complement(1233..1919)
                     /locus_tag="VFI55_02390"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7072916.1"
                     /translation="MTQPVDFEFETAYRGKSAQFGEGVRPPWSIGIPQPELAALIEQG
                     KFHGDVLDVGCGEAAVSLALAERGYTTVGLDLSPTAIDLARREAAKRGLTNATFAVAD
                     ISDFATFPPESAGRFGTIVDSTLFHSIPVEAREGYQQSIVRAAAPGASYFALVFDRAA
                     VTEGPINAVTSDELRDAVAKYWVVDEIRPGRLYAKAPKDFFGMPGMEVREESDGFLSV
                     GGWLLSAHLG"
     gene            complement(2008..3234)
                     /gene="menJ"
                     /locus_tag="VFI55_02395"
     CDS             complement(2008..3234)
                     /gene="menJ"
                     /locus_tag="VFI55_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone reductase"
                     /protein_id="HET7072917.1"
                     /translation="MDVELGADVVVVGAGPAGSAAAGWAARAGNDVLVIDSASFPRDK
                     ACGDGLTPRAVAELERLGLGDWLDTRIRHRGLRMSGFGGEVEVGWPGPSFPSTGSAVA
                     RLELDDRIRKCAEDSGARMLLGTKAVAVHHDSSRRVVSLTLADGTEVGCRRLIVADGA
                     RSSLGRKLGRRWHQETAYGVAARGYLTSPRADDPWLTSHLELRSPDGAVLPGYGWIFP
                     LGNGEVNIGVGALSTSKRPADLALRPLISYYADLRRDEWGFTGEPRAVSSALLPMGGA
                     VSGVAGPNWMLIGDAAACINPLNGEGIDYGLETGRLAAEVLDSSDLSQLWPSLLQQHY
                     GRGFSVARRLALLLTFQRFLPTTGPIAMRSTTLMTIAVRVMANLVTDDDADWVARAWR
                     GGGRLSRLIDRRTPFS"
     gene            3250..4266
                     /gene="grcC1"
                     /locus_tag="VFI55_02400"
     CDS             3250..4266
                     /gene="grcC1"
                     /locus_tag="VFI55_02400"
                     /EC_number="2.5.1.10"
                     /EC_number="2.5.1.29"
                     /EC_number="2.5.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0052924 - all-trans-nonaprenyl-diphosphate
                     synthase (geranylgeranyl-diphosphate specific) activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0033384 - geranyl diphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nonaprenyl/(2E,6E)-farnesyl/geranylgeranyl
                     diphosphat synthase"
                     /protein_id="HET7072918.1"
                     /translation="MSTPATVVAGIDFGDAEFAATVRDGVGRIEQLMDTELRSSDSVM
                     TDSLTHLFKAGGKRFRPLFTVLSAQIGPKPCTAEVTTAGAVIELVHLATLYHDDVMDE
                     AEVRRGTPTANVRWSNNVAILAGDYLFATASRLVARLGPDAVRLIAETFAQLVTGQMR
                     EVRGIVGGVDGEDPVEHYLKVVYEKTACLIAAAGEFGAMFSGAGPDQVQRLSRLGGIV
                     GTAFQISDDIIDIDSDSQESGKLPGTDVREGVRTLPMVYALREPGPEAARLRELLAGP
                     IDDDAEVAEALTLLRASPGMAKAKDFLARYAGQAHQELALLPDGPGRRALETLVDYTV
                     SRHG"
     gene            4383..5243
                     /gene="htpX"
                     /locus_tag="VFI55_02405"
     CDS             4383..5243
                     /gene="htpX"
                     /locus_tag="VFI55_02405"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HET7072919.1"
                     /translation="MTWHPHANRLKTFTLLVGMSALIVFVGALFGRTTLLFATLFAVG
                     MNIYVYFNSDKLALRAMHAQPVSEVQAPAMYRIVRELANAARQPMPRLYISDTNAPNA
                     FATGRNPRNAAVCCTTGILGLLNERELRAVLGHELSHVYNRDILISCIAGAMASVITA
                     LANMAMWAGMFGGNRDGENPIALLLVSFLGPIAATVVRLAVSRSREYQADESGAVLTG
                     DPLALASALRKISGGVQAAPLPPEPQLASQAHLMIANPFRAGERMGSLFSTHPPIEDR
                     IRRLEAMARE"
     gene            5357..5713
                     /locus_tag="VFI55_02410"
     CDS             5357..5713
                     /locus_tag="VFI55_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HET7072920.1"
                     /translation="MSRYTYRLEWLPDYGEYVGVCIELPYLRRDAPTAQLAAAAIEQA
                     VAEHVHALKASGESIPTPLSERSYSGTIVVRTSPQLHARLALEATEQRVSMNQWIVQK
                     LSGRTPSDTFGLSGFD"
     gene            complement(5731..7287)
                     /locus_tag="VFI55_02415"
     CDS             complement(5731..7287)
                     /locus_tag="VFI55_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HET7072921.1"
                     /translation="MQNTERLGGQDIGQDRKESRIGRIRPVIRHDVPASLVVFLVALP
                     LSVGIAIASNAPVLAGLIAAIVGGIIAGAVGGSPLQVTGPAAGLTVVVADLISDFGWG
                     VTCLITATAGVLQVLLGLSRVARAALAISPVVVHAMLAGIGLTIALQQTHVLLGGKSK
                     STAWHNLIDLPGQIIGAHRPGALLGALVIGILIAWRWVPTRLRSVPGPLVAIVAVTAI
                     SEVFPFHVRRIDLDGSPLDALQLPDLPDGNWGAVTIGVITVALIASVESLLSAVSVDR
                     MHHGSRTDFNRELIGQGAANIVSGTVGGLPVTGVIVRSSTNVNAGAKTRASAVLHGVW
                     ILLFTVPFSELVEEIPTAALAGLLIVIGVQLLKPAHIETASKHGDLAVYVVTAVCVIF
                     LNLLHGVIIGLALAIALTGWRVIRARVEACSVDGEWHVAIEGAACTFLALPRLTRVLT
                     SIPSGTVVTVDISAHYLDHAADQAITDWRYQHEATGGTVRIRRTVQPGHLALRRPPNH
                     VKPEQPGAAA"
     gene            complement(7521..8546)
                     /locus_tag="VFI55_02420"
     CDS             complement(7521..8546)
                     /locus_tag="VFI55_02420"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET7072922.1"
                     /translation="MAAEKREPEVVVLGGGSWGTTVASICARRGPTLQWVRSEATAKD
                     INEHHRNSRYLGNDVILSDTLRATTDFVEAANCADVVVMAVPSHGFRGVLTELARELR
                     PWVPVVSLVKGLEQGTNMRMSQIIEELLPGHPAGILAGPNIAREVAEGYAAAAVLAMP
                     DQHLATRLSGLFRTRRFRVYTTDDVIGVEMAGALKNVFAIAVGMGYSLGIGENTRALV
                     IARALREMTKMGVAMGGHPDTFPGLAGLGDLIVTCTSQRSRNRHVGEQLGAGKQIDEI
                     IASMNQVAEGVKAASVVMEFAAEFGLNMPIAREVDSVINHGSSVEDAYRGLAAEVPGH
                     EVHGSGF"
     gene            complement(8605..>9083)
                     /locus_tag="VFI55_02425"
     CDS             complement(8605..>9083)
                     /locus_tag="VFI55_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7072923.1"
                     /translation="DIVITATGLQLQALGGATISRDGTEIDHRDRFVYKAHMLEDLPN
                     LFWCVGYTNASWTLRADITARATAKLLAHMASHGYTHAYPHRGNEPMAVKPSWDIQAN
                     YVKRSAHALPKSGTKRPWNVRQNYLADAIDYRFDRIDEAMVFGRVADRAPVATQPG"
BASE COUNT         1495 a         3004 c         3083 g         1501 t
ORIGIN      
        1 gtccgaagcc tcgcgatgta cccgttatga accacacttt ttcactcacg tagccgtaga
       61 acgcacccac ggccgcgcca ttcccgaaat gaccggatcc cgctgtacgg gctggatggt
      121 cgccgggacg gtaccgtcta cgtcgtgagt cgcgcggcct tggacaagga cccccgggac
      181 gttgcctcga tgttcgacgg cgtcgcccgc cggtatgacc tgaccaacac cgtgctgtcg
      241 ctgggtcagg accggttctg gcggcgcgcc acccggtcgg cgctgggggt cgggcccggt
      301 cagaaggtgc tggacctggc cgcgggcacc gcggtatcca ccgtggagct cacgaaatcc
      361 ggggcatggt gtgtggcggc cgatttttcg gtcggaatgc tggccgcggg cgctgcgcgc
      421 gacgtgccga aggttgccgg tgacgctacc cggctgccgt tctgcgacgg ggtattcgac
      481 gcggtcacca tcagtttcgg gttgcgcaac gtggccgacc agccggcggc gctgcgcgag
      541 atgctccgcg tcacccgacc cggcgggcgg ttggtggtgt gcgaattctc cacgccgacc
      601 aatgggctgt tcgccaccgc ctacaaggag tacctgatgc gggcgctgcc gcgggtggct
      661 caggccgtgt ccagcaaccc cgacgcgtac gtctacctcg ccgagtccat cagggcctgg
      721 cccgaccagg ccaccctggc gcaccggatc tcggcagccg ggtggtcggc tgtgcgctgg
      781 cgcaacctga ccggtggcat cgtggcattg cacgccggat acaagcccct tcgacaaggc
      841 cagtgacgag aacttagcga gtgccggccc gttgcaggac gccgacctgg tcggggcagt
      901 agtggtcgat cgcagccccc aggaactgga acgattgccc ctcggtggtg tgcagcggca
      961 ggttgcgctg aataaaggtg gccgacttgt acgcgtcgcc gtccacgccg cgggacagtc
     1021 gttcgcaggt gatcttgccc agccaggcgt tgtagtcctg tggaccgtaa atgccgtagg
     1081 tgtgcagttg cgcgttgaac ggggcgtcgt agtcgtcggc ctgcgccggc gcggccagcg
     1141 cgatcgcccc cgccaatatc ggtgccgcca cagcaacgct ggcggcgagg ctcgtgccct
     1201 tcattagctg gactatacac ctggccgttc agtcagccca ggtgtgccga caacagccag
     1261 ccgccgaccg acaggaagcc gtcggactcc tcgcgcacct ccatgccggg catcccgaag
     1321 aagtccttgg gggccttggc gtagaggcgg cccggccgga tctcgtcgac aacccagtac
     1381 ttggccaccg cgtcgcgcag ctcgtcgctg gtcaccgcgt tgatcgggcc ttcggtcacc
     1441 gccgcccggt cgaagaccag cgcgaaatag gacgcgccgg gggccgcggc ccgcacgatc
     1501 gactgctggt agccctcgcg cgcctccacg gggatggaat ggaacagcgt gctgtcgacg
     1561 atggtgccga atcggccggc cgattcggga gggaacgtgg cgaaatcgct gatgtcggcc
     1621 accgcgaagg tggcgttggt gagcccgcgc ttcgcggcct cgcggcgggc caggtcgatg
     1681 gcggtgggag acaggtcgag ccccacggtg gtgtaccccc gttcggccag cgccagcgag
     1741 acggccgcct ccccgcagcc cacgtccagc acgtcgccgt ggaacttgcc ctgctcgatc
     1801 agggcggcca gctcgggctg ggggatcccg atgctccagg gcggacggac gccctcaccg
     1861 aattgggcgg acttgccgcg ataggccgtt tcgaactcga aatctactgg ctgagtcatg
     1921 tcatcaggta tatcaacacg cctgatatat gtcaaccaga ttgatatatg gatcgtgcac
     1981 cacccgttgt ccaccgagaa aactggctca gctgaacgga gttcggcgat ctatcagccg
     2041 ggacagccgc ccgccgcccc gccaggcccg cgccacccag tcggcatcgt cgtcggtgac
     2101 caggttggcc atcacccgca cggcgatggt catcagcgtc gtcgagcgca tcgcgatcgg
     2161 cccggtggtg ggcaggaagc gctgaaaggt cagcagcaac gccagcctgc gggcgaccga
     2221 aaatccgcgg ccgtaatgct gctgcagcag cgatggccac agctgcgaca ggtcgctgct
     2281 gtctagcacc tccgcggcca gccggccggt ctccaagccg tagtcgatgc cttcgccgtt
     2341 cagcgggttg atgcacgccg cggcgtcgcc gatcagcatc cagttggggc cggccacccc
     2401 ggagaccgcg ccgcccatcg gcagcagcgc cgaggacacc gcccgcggct cgccggtgaa
     2461 accccactcg tcgcggcgca ggtccgcgta gtaggagatc agcggtcgca gcgccagatc
     2521 cgccggtcgt ttggacgtcg acagggcgcc gacgccgatg ttcacctcgc cgttgcccag
     2581 cgggaagatc cacccgtagc cgggcagcac ggcgccgtcg ggggaacgca gttccaggtg
     2641 cgacgtcagc caggggtcgt cggcgcgcgg actggtcaga tacccgcggg cggcgacgcc
     2701 gtaggccgtc tcctggtgcc agcgccggcc cagttttcgg ccgagcgatg agcgggcacc
     2761 gtcggcaacg atcagccggc ggcagcccac ctcggtgccg tcggccagcg tgagcgatac
     2821 cacccgtcgc gacgagtcat ggtgcacggc aacggccttg gtcccaagga gcatccgtgc
     2881 cccggaatcc tcggcgcatt tgcggatccg gtcgtccaac tcgaggcggg ccaccgcgct
     2941 gccggtcgac gggaacgacg ggccgggcca gccgacttcc acctcgccgc cgaatccgct
     3001 catccgcagt ccgcgatgcc ggatgcgggt gtccagccag tcgccgagcc ccagtcgctc
     3061 cagttcggcg accgcgcgcg gcgtcagccc gtcgccgcag gccttgtcgc gggggaagct
     3121 ggcggagtcg atgacgagga cgtcgttgcc cgcgcgggcg gcccaaccgg ctgccgccga
     3181 acccgcaggt ccggccccca cgaccaccac atccgcgccg agttccacat ccaccagtat
     3241 gttggtcggg tgagtactcc ggcgacggtg gtggcgggca ttgactttgg cgatgccgaa
     3301 ttcgccgcga ctgtgcgcga cggtgtgggg cgaatcgagc agctcatgga caccgagctg
     3361 cgcagctcag actcggtaat gaccgattcg ctgacgcacc tgttcaaggc cggcggcaaa
     3421 cggttccggc cgctgttcac cgtgctgtcg gcgcagatcg ggcccaagcc gtgtaccgcg
     3481 gaggtgacaa ccgccggtgc ggtcatcgag ctggtgcacc tggccacgct ctaccacgac
     3541 gacgtgatgg acgaggcgga ggtccgccgc ggcacgccca ccgccaatgt gcgctggagc
     3601 aacaacgtgg cgatcctggc cggcgactat ctgttcgcga cggcgtcgcg gctggtggcc
     3661 cggctgggcc cggacgcggt gcggctgatc gccgagacgt tcgcccagct ggtgaccggg
     3721 cagatgcgcg aggtgcgggg catagtcggc ggggtcgacg gggaggatcc ggtcgagcac
     3781 tacctgaagg tggtgtacga gaagacggcg tgcctgatcg cggcggccgg cgaattcggc
     3841 gcgatgttct ccggtgccgg ccccgatcag gtccagcggc tgagccgcct tggcggcatc
     3901 gtcggcaccg cctttcagat ctccgacgac atcatcgaca tcgacagcga ttcccaggag
     3961 tccggcaagc tgcccggtac cgacgtgcgc gaaggcgtgc gcaccttgcc gatggtctac
     4021 gcgctgcgag aacccggccc cgaggcggcg cggctgcgcg agctgctggc cggacccatc
     4081 gacgacgacg cggaggtggc cgaggcgctg acgctgttgc gcgcatcgcc tggcatggcc
     4141 aaggccaagg acttcctggc acggtacgcg ggccaggcac accaggagct ggcccttttg
     4201 cccgacggtc ccggtcggcg cgccctcgag acgctggtcg actacacggt cagccggcac
     4261 ggctagcgct gattgccgcg ctttcctgtg gtggcgcgga acccggaagg tcatcccggg
     4321 cgttcaatga gcgatagtca gcaatgaggc gagtgttctt gagtgtttgg aggagtacac
     4381 cgatgacctg gcatccccac gccaacaggt tgaagacctt caccctgttg gtcggtatgt
     4441 ccgcgttgat cgtgtttgtc ggtgcgttgt tcggcaggac gacgctgctt ttcgccacgc
     4501 tcttcgcggt cggcatgaac atctacgtgt acttcaacag cgacaagctg gcgttgcggg
     4561 cgatgcacgc gcagcccgtt tccgaagtgc aggcgccggc gatgtatcgg atcgtgcgcg
     4621 agctggccaa cgccgctcgc cagccgatgc ccaggctgta catcagcgac accaatgcgc
     4681 ccaatgcgtt cgccaccggc cgcaacccgc gcaacgccgc ggtgtgctgc accaccggca
     4741 tcctcggtct cctcaacgag cgcgagctgc gcgccgtgct gggacacgag ctgtcgcacg
     4801 tctacaaccg cgacatcctg atctcctgta tcgccggcgc gatggcatcg gtgatcaccg
     4861 cgctggccaa catggccatg tgggccggca tgttcggcgg caaccgggat ggcgagaatc
     4921 cgattgcgct gttgctggtt tcgtttttgg gcccgattgc cgcgacggtg gtgcggctgg
     4981 cggtgtcccg gtcgcgcgaa taccaggcag acgaatcggg cgccgtgctg accggggatc
     5041 cgctggccct ggcgtcggct ctgcgcaaga tctccggcgg ggtccaggcg gccccgctgc
     5101 ctccggagcc gcagctggcc agccaggcac atctgatgat cgccaacccg ttccgcgcgg
     5161 gcgagcgcat gggatcgctg ttctcgacgc acccgccgat cgaagaccgc atccgccgat
     5221 tggaggccat ggcgcgcgag tagcgatagc gcatgcgcta tcaaaatcgc agcaggccgc
     5281 atgccctgaa gaaccgctct cgccctgggg ataagtgact cccgaaagcg atagcgtatg
     5341 tgctaccaaa acccgcgtga gccgctacac ctaccgcttg gaatggttgc ccgattatgg
     5401 cgagtacgtg ggtgtgtgca tcgagctgcc gtacttgagg cgcgacgcgc cgacagcgca
     5461 gctggccgcc gccgcgattg agcaagccgt cgctgagcac gtgcacgccc tgaaggcaag
     5521 cggagagtcc atccccacac cactttccga gcgcagctac agcgggacga tcgtcgtcag
     5581 gacatcaccg cagctgcatg cccggctcgc cttggaggcc actgagcaga gggtttcgat
     5641 gaaccagtgg atcgttcaga agctcagcgg acggacgccg agcgatacct tcgggctgtc
     5701 ggggttcgat taggggccgg cgtccgccga tcaggcagca gccccaggtt gttcgggctt
     5761 tacgtggttg ggagggcgcc gtagggcgag gtggccgggc tggaccgttc ggcgaatccg
     5821 gacggtgccc cccgtggcct catgctggta ccgccagtcg gtgatcgcct gatccgcggc
     5881 gtggtccagg taatgggccg aaatgtcgac ggtgacaacc gttccggagg ggatggacgt
     5941 cagcacacgc gtcagccggg gcagggcgag aaaggtgcag gccgcccctt cgatggctac
     6001 gtgccactcg ccgtctaccg aacacgcctc gaccctggcg cggatcaccc gccatccggt
     6061 cagcgcgatg gccagggcga gtccgatgat cacgccgtgc agcagattga ggaatatgac
     6121 gcaaaccgcg gtcaccacgt agacggcgag atccccatgc ttgcttgcgg tttcgatatg
     6181 cgccggcttc agcagctgaa ccccgatgac gatcagcagg ccggcgagtg cggcggtcgg
     6241 gatttcttcg accaactccg agaacggaac cgtgaacagc aggatccaga cgccgtgcag
     6301 gacggccgat gcgcgggttt tcgcgcccgc gttgacattg gttgaactgc ggacgattac
     6361 accggtgacc ggcagcccgc cgacggtgcc cgacacgatg ttcgcggcgc cctggccgat
     6421 caactcgcgg ttgaagtcgg tgcgtgaccc gtggtgcatc ctgtcgacgg aaaccgctga
     6481 gagcaggctc tcgacgctgg cgatcagggc gacggtgatc acgccgatcg tgaccgcgcc
     6541 ccagttgccg tccggaaggt caggcagttg cagcgcatcc agcggtgacc catcgaggtc
     6601 gatccgtcgt acgtggaatg ggaagacctc cgagatggcc gtcacggcca cgatcgcgac
     6661 gagtgggcct ggaacagaac gcaatcgagt cggaacccac cgccaggcga tcaggatgcc
     6721 gatcaccagc gcgcccagca gcgccccggg ccggtgggcg ccgatgatct ggcccggcag
     6781 gtcaatgagg ttgtgccagg ccgtgctttt ggatttcccg cccagcaaga cgtgcgtctg
     6841 ttgcagtgcg atcgtgagtc cgatgccggc cagcatggca tgcacgacga cgggtgagat
     6901 cgccagtgcg gcccgcgcga cacggctgag ccccaacagg acctgcagca caccagcggt
     6961 tgcggtgatc aaacacgtca ccccccaacc gaagtcggag atcagatcgg caacgacgac
     7021 ggtcagcccg gccgcgggac cggtgacctg cagcggtgat ccgccgacgg ctccggcgat
     7081 gattcccccg acgatcgctg cgatcagacc ggcgagcacc ggagcgttcg acgcgatcgc
     7141 gatgcccacc gacaatggaa gtgcgaccag gaaaaccacg agcgacgcgg gcacgtcgtg
     7201 ccggataacc ggtcttatcc ggccaattcg cgattccttg cggtcctggc caatatcctg
     7261 accgcccagt cgttcggtgt tttgcatcgg ttgcttccct cgttaggtct agcgggggat
     7321 gttccgatac ccgcccctac tgcgcttcgt cgcgtcggcg tagctcattg gcgcacaagt
     7381 gattacagcc gcgccagtcg gcaggtctaa gcctgggttc ctcgttcgag agtattttgg
     7441 cggcgaagcg cgggtcaagg cgcgcagccg ggcgcatatc gaatgcaaat cttctcctca
     7501 gcacgagagg agtcgccggg tcagaacccc gagccgtgga cttcgtgtcc gggaacctca
     7561 gcggccagtc cgcgataggc gtcctcgacg ctggacccgt ggttgatcac agagtcgact
     7621 tcgcgcgcga tcggcatgtt cagaccgaac tcggcggcga attccatgac cacgctggcg
     7681 gccttgacgc cctcggcgac ctggttcatc gacgcgatga tctcgtcgat ctgtttgccc
     7741 gcgccgagtt gctcgccgac gtggcggttg cggctgcgct ggctggtgca ggtgacgatg
     7801 aggtcgccga ggccggccag cccggggaag gtgtcggggt gtccgcccat cgctacaccc
     7861 atcttcgtca tttcgcgcag ggcgcgcgcg atcaccagtg cgcgggtgtt ttcgccgatg
     7921 cccagcgaat aacccatccc gacggcgatg gcgaagacgt tcttcagcgc gccggccatc
     7981 tcgaccccga tgacgtcgtc ggtggtgtag acgcggaacc gccgggtgcg gaacagtccc
     8041 gagagtcggg tggccagatg ctgatcgggc atggccagca ccgccgcggc ggcgtaaccc
     8101 tcggccacct cacgggcgat gttcgggccg gccagtatgc ccgcgggatg accgggcagc
     8161 agctcctcga tgatctgcga catccgcatg ttggtgccct gctcgagccc cttgaccagc
     8221 gacacaaccg gcacccatgg ccgcagttcc ctggccagtt cggtgagcac accccggaag
     8281 ccgtgcgagg gcaccgccat gaccacgacg tcggcacagt tcgcggcctc gacaaagtcg
     8341 gtggtcgcgc gaagcgtgtc gctcaggatg acgtcattgc cgagatagcg gctgttgcgg
     8401 tgatgctcgt tgatgtcctt ggcggtcgcc tccgagcgca cccactgcag ggtcggcccg
     8461 cgtcgcgcgc agatcgacgc cacggtggtt ccccaggaac cgccacccag cacaacgact
     8521 tcgggttcgc gcttctcagc tgccatggcg atcagcctat tgccgtaacc agacatttag
     8581 tagcagttca accggagtcc gggatcagcc cggctgcgtc gccaccgggg cccggtcggc
     8641 gactcgtccg aagaccatcg cctcgtcgat gcggtcgaac cggtagtcga tggcgtcggc
     8701 gaggtagttc tgccgcacgt tccacggccg cttggtgcct gacttcggca gcgcatgcgc
     8761 ggaccgtttc acgtagttgg cctgaatgtc ccaggacggc ttcacagcca tcggctcgtt
     8821 gccccggtgc gggtaggcgt gggtgtagcc gtgagaggcc atgtgcgcca acaacttcgc
     8881 cgttgcccgg gccgtgatgt cggcccgcag cgtccaggac gcgttggtat agcccacgca
     8941 ccagaacagg ttcggcaagt cttcgagcat gtgcgccttg tagacgaagc gatcgcggtg
     9001 atcgatctcg gtgccgtcgc ggctgatcgt ggccccgccc agcgcctgca gctgcaggcc
     9061 ggtggccgtg atgacgatgt cgg
//
LOCUS       DASSGQ010000057         2624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_186444,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000057 DASSGQ010000000
VERSION     DASSGQ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186444"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..2392)
                     /locus_tag="VFI55_02430"
     CDS             complement(80..2392)
                     /locus_tag="VFI55_02430"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET7072924.1"
                     /translation="MERVRLAARWRGLISTAAVVLGAALVTQAGPSPAGASTLPTGFR
                     DSVVLSGLTNPTVLQFAPDGRIFVGQKNGVIKVFQSLTDTNPVTVADLSGEVDDYWDR
                     GLLGLALPPNFPASPYVYVLYAYDAPIGGTAPAWGDACPTPPGPTTDGCLVSARVSRL
                     PISGNVMTGSEQVLINDWCQQFPSHSIGTLLFGRDGYLYVSGGDGASFNNVDYGQYGA
                     NYAGDQANPCGDPPGAAGAALSPPGAEGGALRSQSVRRTDGPATLDGAVLRIDPANGA
                     GVPGNPFYSSPDANARRIVAYGLRNPFRITQRPGTDELWIGDVGWNTWEEIDRVTSPA
                     NTSASNFGWPCYEGASAQGGYQGAGLNLCSSLYSAPGSVIAPYYTYNHGACVVSYTGC
                     HTGGSSITGVAFYQGGSYPAQYNGALFFGDHTRNEIWAMLAGANGLPDPSRLQSFVGV
                     DAAGGAAGHPVDLKIGPGGDLFYVDMDDGTVHRITFTAANQPPTAMITASPTNGPLPL
                     TVSFDGTGSSDPEGKPLSYSWDLNGDGTFGDATGPTASYAYTTAGVYHATLRVTDDQG
                     ASDTATVTITAGNTAPTAVIDSPTSSLTWKVGDAISFSGHAADAQDGTLPASKLSWSL
                     ILHHCFTPTDCHTHLIQTLSGVSSGLFTAPDHEYPCWLELQLTATDSGGLTSITSVRL
                     DPKTVVLTFKTNPGGLVLSDLVVNEAPRTTPFSVTVVVGSANSVSAPSPQQFNKSTYF
                     FSSWSDGGPQSHTITAPAANTTYTATYRKR"
BASE COUNT          419 a          856 c          925 g          424 t
ORIGIN      
        1 gcggcgaagt agtgcgttct agttgttcag tctccacggt ttcaggggaa cccgtccgtc
       61 cggcgccgcg ggtcgcatct cagcgcttgc ggtatgtcgc ggtataggtg gtgttcgccg
      121 cgggcgcggt gatggtatgg ctctgcgggc cgccgtccga ccaggagctg aagaagtagg
      181 tggacttgtt gaattgctgc ggtgatgggg cgctgaccga gttggccgag ccgaccacca
      241 cggtcacgga gaacggcgta gtacgcggcg cctcgttgac gaccaggtcg ctgaggacga
      301 gacctcccgg gttggtcttg aacgtgagca cgaccgtctt ggggtccagc cggacgctgg
      361 tgatggaggt cagcccgcct gagtcggtcg cggtcaattg cagttcgagc cagcacgggt
      421 attcatggtc cggcgcggtg aacaatccgc tggagacacc gcttagggtc tggatcaggt
      481 gcgtgtggca gtcggtcggc gtgaagcagt gatgcaggat cagcgaccag ctcagcttcg
      541 acgccggcag cgtcccgtcc tgcgcgtcgg cggcgtgacc ggagaagctg atcgcgtcgc
      601 cgaccttcca ggtcaggctc gatgtcggcg agtcgatgac cgcggtcggc gcggtattgc
      661 cggccgtaat ggtgaccgtc gccgtgtcgc tcgcaccctg gtcgtcggtg actcgcaggg
      721 ttgcgtgata gacgcccgcc gtggtgtagg cgtacgacgc cgtcgggccg gtcgcatcgc
      781 caaacgttcc gtcgccgttg agatcccacg agtagctcag cggcttaccc tccgggtcgg
      841 acgagccggt gccgtcgaag ctgaccgtca gcggtagcgg accgttggtc gggctcgccg
      901 tgatcattgc ggtcggcggc tggttggcgg ccgtgaaggt gatgcggtgg acggtgccgt
      961 cgtccatgtc gacgtagaac aggtcgccgc cggggccgat cttcaggtcg acgggatgcc
     1021 ctgcggcgcc gcccgccgcg tcgaccccga cgaacgactg cagcctggac gggtcgggca
     1081 acccattcgc gccggcgagc atcgcccaga tctcgttgcg ggtgtggtca ccgaagaaca
     1141 gcgccccgtt gtattgcgcc ggatacgacc cgccctggta gaacgccacc ccggtgatgg
     1201 acgagccgcc ggtgtggcag ccggtatagc tcaccacgca cgcgccatgg ttgtaggtgt
     1261 aatagggcgc gatgaccgaa ccaggcgccg agtagagaga cgagcacaga ttgaggccgg
     1321 ccccctggta accgccttgc gctgacgctc cctcatagca cggccagccg aagttcgacg
     1381 cgctggtatt cgccggcgat gtcacgcggt cgatctcctc ccaggtattc cagccgacgt
     1441 cgccgatcca cagttcgtcg gtgcccggac gctgggtgat gcggaacggg ttgcgcagcc
     1501 cgtaggccac gatccgccgc gcgttggcat ccggcgagga atagaagggg ttgccgggca
     1561 cgcccgcacc attggcggga tcgatccgca gaaccgcgcc gtcgagggtt gccgggccat
     1621 cggtacgccg gacgctctgg ctgcgcagcg cgccgccctc cgctccgggc gggctcagcg
     1681 cggcgccggc cgcgccgggc ggatcgccac agggattggc ctggtccccg gcataattcg
     1741 cgccgtactg cccgtagtca acgttgttga agctggcgcc atcgccgcca ctgacgtaga
     1801 ggtacccgtc gcggccgaac agcagcgtgc cgatcgaatg gctggggaac tgctggcacc
     1861 agtcgttgat gagcacctgc tcgctgccgg tcatgacgtt cccactgatc ggcagccgcg
     1921 acacgcgcgc cgacacgaga cagccgtcgg tggtcgggcc cggcggcgtc gggcaggcat
     1981 cgccccaggc cggggccgtg ccgccgatcg gggcgtcata cgcgtagagc acgtagacgt
     2041 aagggcttgc ggggaaatta gggggcagcg ccaagccgag caggccacgg tcccagtaat
     2101 cgtcgacctc gcccgacaag tcggcaacgg tgaccgggtt cgtgtcggtc aggctctgaa
     2161 agaccttgat cacgccgttc ttctggccga cgaagatccg cccgtcgggt gcgaattgca
     2221 gcaccgtcgg gttggtcagc ccgctcagca cgaccgaatc gcgaaacccg gtgggcaacg
     2281 tggacgcacc cgccgggctg ggcccggcct gcgtgacgag cgccgcgccc agcaccaccg
     2341 ccgccgtact gatcagcccg cgccaacgcg cggcaagcct cacacgctcc atgccgcccc
     2401 ccggcgatcc gctccgcagc gcacggccgc ttccgcgcgc cgctccaccc ctattctgct
     2461 cgccgggcaa gcactcggtc aggaatttgg caaattactc acatcgcatg tgcggtaaac
     2521 cagccgacag ggcggcggat accgggaacg cacccgggac cagaggcgtg cgacggaaaa
     2581 tccggtccgt gcggtgcagg agcggcgccg ccgacgaacg tggc
//
LOCUS       DASSGQ010000058         7113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1006629,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000058 DASSGQ010000000
VERSION     DASSGQ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7113
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006629"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VFI55_02435"
     CDS             <1..786
                     /locus_tag="VFI55_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET7072925.1"
                     /translation="PPEPIPDVPAWDSVLASRRPDRPGVGYLLRHATTDPEPLSGIGL
                     GEAATTLLALARFNGQPAVVLGQQRVTGGLVGPASLRAARRGMALAAELRLPLVLVID
                     TAGPALSADAEEGGLAGQIAQCVAELVTLDTPTVSVLLGQGSGGPALSMLPADRVLAA
                     LHGWLAPLPPEGASAIVFRDTAHAPELAAAQGIRSADLLASGIVDAIVPEHPDAADEP
                     LEFSQRLSRAIAAELHALRDIPARERLAARLRRYRRLGLTGDA"
     gene            complement(791..1342)
                     /locus_tag="VFI55_02440"
     CDS             complement(791..1342)
                     /locus_tag="VFI55_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET7072926.1"
                     /translation="MRRSSEETKALILAAARERFGAAGFQAATIRAVAADAGVDPAMV
                     MRYFGSKDKLFAAAAEFDLRFPDYATTDPTQVGQSLVRHFLERWEGDEALVILLRSSA
                     TNGEAAQRMQEIFGSQLQPLVASLVPVEECAARAGLIATQILGMALCRFVLRLPPIVG
                     MTRDEIVEWIGPTIQRYLGLPEG"
     gene            1453..2592
                     /locus_tag="VFI55_02445"
     CDS             1453..2592
                     /locus_tag="VFI55_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7072927.1"
                     /translation="MNDTDVLVVGAGPTGLTLTASLIANGVRAVVVDKLPEGQNTTRA
                     AAVNARTLEVLEDLDVARRMVKAGVIAPRFTMREGRRVLIPVDFSKLPTKYPYTLMLS
                     QADTERLLLQRLRELGGGVIRPKTLSGVTQDADGVTATFDHGDTIRAGYVVGTDGMHS
                     TVRDQAGIGFTGGEFAESFALADVRLAGDAPRDEVILFYAREGLNVLAPLPGDIFRIV
                     APVTDAPKVPSTAFVQALLDNRAFGPGQLVVTELQWGTRFHIHHRVADAYRSGRLLLA
                     GDAAHVHSPAGGQGMNLGITDAISLAGALTEVLNGRSDSALDAYGTAQRQRAAQVLKL
                     TGRLTRIATLPRPLRPVRNSGMRLAAEVPAVRRQLALQLSGLGRR"
     gene            2693..3403
                     /gene="prrA"
                     /locus_tag="VFI55_02450"
     CDS             2693..3403
                     /gene="prrA"
                     /locus_tag="VFI55_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PrrA"
                     /protein_id="HET7072928.1"
                     /translation="MGGMDTGATSPRVLVVDDDADVLASLERGLRLSGFEVSTAVDGA
                     EALRSATETRPDAIVLDINMPVLDGVSVVTALRAMDNDVPVCVLSARSSVDDRVAGLE
                     AGADDYLVKPFVLAELVARVKALLRRRGATATSSSETITVGPLEVDIPGRRARVNGVD
                     VDLTKREFDLLAVLAEHKTAVLSRAQLLELVWGYDFAADTNVVDVFIGYLRRKLEANG
                     GPRLLHTVRGVGFVLRMQ"
     gene            3431..4771
                     /locus_tag="VFI55_02455"
     CDS             3431..4771
                     /locus_tag="VFI55_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7072929.1"
                     /translation="MKILTRVFARTPSLRTRVVIATAIGAAIPVLIVGTVVWVGITND
                     RKERLDRRLDEAAGFAIPFLPRGLDEIPRSPNDRDAIITVRRSGLVKSNSDVTLPQLN
                     ADYADTYVNGVRYRVRTVAIPGPEPTSLAVGATYDTTLAETNNLHRRVLLICGFAIGA
                     AAVFAWLLAAFAVRPFKQLAEQTRAIEAGDEAPRVEVHGASEAVEIAEAMRGMLRRIW
                     DEQNRTKEALASARDFAAVSSHELRTPLTAMRTNLEVLATLDLPDDERKEVLNDVIRT
                     QSRIEATLSALERLAQGELSTADDHMPVDITELLDRAAHDAMRVYPELDVSLVPSPTC
                     IIVGLPAGLRLAVDNAIANAVKHGGASRIQLSAVSSRAGVEIAIDDNGVGVPETERQV
                     VFERFSRGSTASHSGSGLGLALVAQQAQLHGGTASLENSPLGGARLVLRLPAPC"
     gene            complement(4788..5015)
                     /locus_tag="VFI55_02460"
                     /pseudo
     CDS             complement(4788..5015)
                     /locus_tag="VFI55_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215416.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5726..5878)
                     /locus_tag="VFI55_02465"
     CDS             complement(5726..5878)
                     /locus_tag="VFI55_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072930.1"
                     /translation="MDFVIQWLWYLLAFVAGSAVARVIANIVNKRAGAEIVPGDATGP
                     GAGGQR"
     gene            complement(5890..6912)
                     /locus_tag="VFI55_02470"
     CDS             complement(5890..6912)
                     /locus_tag="VFI55_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015292520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7072931.1"
                     /translation="MGLGAGLRQAAATTDSRRAPESGWRSLGRPWLIAVVVIPLLIAA
                     IGYGAYERPRSVNGPTGAVPTLSPSSKSGAPKFSLAPLSITRNGNTITLTGDFPDDSA
                     KAALMKALTGSLAPGVNIVDQIHINPNVDALDFSNAGAIFKDSASIPDFSLTVNGDSI
                     TLAGTAASQDQKNTVEQETAHTWSNLNVVDRLVVNAANPPPPPAKPGAPPPPPPPPPA
                     PGPCADLQSAINAATGGPITFGNDGFSLTPADEQILTQVADKLKACPNAHATINGYTD
                     NSGTEAINIPLSNQRAQTVADFLTAHGVAAAELVVTGLGSLNPVAPNDTVEGRAKNRR
                     VEIVVS"
     gene            7019..>7113
                     /locus_tag="VFI55_02475"
     CDS             7019..>7113
                     /locus_tag="VFI55_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 domain-containing protein"
                     /protein_id="HET7072932.1"
                     /translation="MGNGKKPTDSETLAHIRGLVAEEKALREQLQ"
BASE COUNT         1114 a         2392 c         2430 g         1177 t
ORIGIN      
        1 ccacccgagc cgatacccga cgtgcccgcg tgggactcgg tgctggcatc ccgacggcca
       61 gaccggccgg gcgtcggata cctgctgcgg cacgccacga ccgacccgga gccgctgtcc
      121 ggcatcggcc tgggcgaggc ggcgaccacg ttgctggcgc tggcccgatt caacgggcag
      181 cccgcggtgg tcctcggcca gcagcgcgtc accggcggac ttgtcggccc cgcttcgctg
      241 cgcgcggcgc ggcggggcat ggcgctggcc gccgagctgc gcctgccgct ggtgctggtg
      301 attgacaccg ccggccccgc gttgtcggcc gatgccgagg agggcggact ggccggccag
      361 atcgcgcagt gcgtcgccga actcgtgacg ctggataccc cgacggtttc ggtcctgctg
      421 ggccagggca gcggtggacc cgcactgtcg atgttgcccg ccgaccgggt gttggccgcc
      481 ctgcacggct ggttggcgcc gttgccgccg gagggcgcca gcgcgatcgt gttccgtgac
      541 accgctcatg cccccgaact cgcggcggcc cagggcatcc ggtcggccga cctgctggcg
      601 tccgggattg tcgacgccat cgtgccggag caccccgacg ccgccgacga gccgctcgaa
      661 ttctcccaac ggctgtcgcg cgcgatagcc gccgagctgc acgcactgcg cgacatcccc
      721 gcccgcgagc ggctggcggc gcgactgcgt cgctaccgcc gcctcgggtt gaccggcgac
      781 gcttaggttt tcagccctcc ggcagcccga ggtagcgctg tatcgtcggg ccgatccact
      841 cgacgatctc gtcgcgggtc atcccgacga tgggcggtag ccgcaacacg aagcggcaca
      901 gcgccatacc gaggatctgc gtggcgatca gcccggcccg ggcagcgcat tcctcaaccg
      961 gcaccagcga cgcgaccaat ggctgcagtt ggctcccgaa aatctcctgc atccgttgcg
     1021 cggcttcccc gttggtggcg ctggaccgca gcaggatcac cagcgcctcg tcaccctccc
     1081 agcgctccag aaagtgccga accaacgact gcccgacctg cgtcgggtcg gtggtcgcgt
     1141 aatcgggaaa cctcaggtcg aactcggcag cggcggcgaa cagcttgtcc ttgctgccga
     1201 agtaccgcat caccatcgca gggtccacgc cggcgtcggc ggcgacggcc cgaatggtgg
     1261 ccgcctggaa gccggccgcg ccgaatcgct cacgggccgc cgcgaggatg agcgccttgg
     1321 tctcctcgga cgatcgtctc atgtcaacaa ttgtaggcca acaactgttg acattgccgc
     1381 agcggcgcgc gaccatgatg gtatgccaac aagcgttgac taactgaatt caatcgtcgc
     1441 gaggagtgga ccatgaacga caccgacgtc ctggtggtag gcgccggccc gaccggcctg
     1501 acgttgaccg catccctgat cgccaatgga gtccgagcgg tggtggtcga caagttgccg
     1561 gaggggcaga acaccacacg tgcagccgcc gtgaacgccc gcacgctcga ggtgctcgaa
     1621 gacctcgacg tggcccggcg gatggtcaaa gccggggtga tcgcgccgcg attcaccatg
     1681 cgggaaggcc gccgcgtcct gataccggtc gacttcagca agctgccgac gaagtacccg
     1741 tacacgctga tgctctccca ggccgatacc gagcgactgt tgctgcagcg actgcgcgag
     1801 ttgggcggtg gcgtgatccg gcccaagacc ctgagcggcg tcacccagga tgcagacggc
     1861 gtgacggcca ccttcgacca cggcgacacc atccgggccg gctatgtcgt cgggaccgac
     1921 ggcatgcaca gcacagttcg tgatcaggcc ggaatcggtt tcaccggagg tgaattcgcg
     1981 gaatccttcg cactcgccga cgtccggctg gccggtgacg cgccgcgcga cgaggtgatc
     2041 ctgttctacg ccagggaagg cctgaacgtg ttggcgccgc tgcccggcga catcttccgc
     2101 atcgtcgccc cggtgaccga cgcgccgaag gttccgtcaa cggcattcgt ccaggcactg
     2161 ctggacaatc gggctttcgg ccccgggcag ctggtggtga ccgaactgca gtggggaacg
     2221 cggttccaca tccaccaccg cgtcgccgac gcctatcggt ccggccggtt gctgctggcc
     2281 ggcgacgcgg ctcacgtgca cagccccgcc ggcggccagg gcatgaatct tggcatcacc
     2341 gacgcgatct ccctggccgg cgcgctcacc gaagtcctga acggcaggtc cgacagcgcg
     2401 ctggacgcct acggcaccgc ccagcgtcaa cgggccgcgc aggtgctgaa actgaccggg
     2461 cggctgaccc ggatcgcgac cctgccgcgc ccgctgcgcc cggtccgcaa ttcgggaatg
     2521 cggctggccg ccgaggtacc cgccgtacga cggcagctgg cgttgcagtt gagcggcctt
     2581 ggacgccggt gatctaccag aggtcgggag tccgtgaatt acccctcccg gcgtcagtcc
     2641 ccccgcgacc cagcggtgat cctcccaccg ccacacgcaa caatcaggca aaatgggcgg
     2701 catggacact ggtgcgacct ccccgcgcgt cttggtcgtc gacgatgacg ccgacgtact
     2761 ggcctcgctg gagcgcggct tgcgcctctc cggattcgag gtgtcgacgg cggtcgacgg
     2821 cgccgaggcc ttgcgcagcg ccaccgagac gcgcccggac gcgatcgtgc tcgacatcaa
     2881 catgcccgtc ctggacggcg tcagcgtcgt cacggcgctg cgggccatgg acaacgacgt
     2941 gccggtctgc gtgctgagcg cgcgcagctc ggtcgacgac cgggtggcgg gcctggaggc
     3001 gggtgccgac gattacctgg tcaaaccgtt cgtcctggcc gaactggtcg cgcgggtgaa
     3061 ggcgctgctg cgccgccgcg gcgccaccgc cacctcgtcg tcggaaacca tcaccgtggg
     3121 cccgctcgag gtggacatcc ccggccggcg ggcccgcgtc aacggcgtcg acgtcgacct
     3181 gaccaagcgg gagttcgatc tgctggccgt gctcgccgag cacaagaccg cggtgctgag
     3241 ccgcgctcag ctgttggagc tggtctgggg atatgacttc gccgcggaca cgaacgtcgt
     3301 cgacgtgttt atcgggtacc tgcgccgcaa gttggaagcc aatggcggcc ccaggctgct
     3361 gcacaccgtc cgcggggtcg ggttcgtgct gcggatgcag taaccatgtg ctgaccagag
     3421 tgaagcggtg atgaagatcc tgacccgggt ttttgcccgt acaccgtcgc tgcggacccg
     3481 ggtggtgatc gcgacggcca tcggcgccgc gattccggtg ctcatcgtcg gcaccgtggt
     3541 ctgggtgggg atcaccaacg accggaagga acggctcgac cgccggctgg acgaggcggc
     3601 gggtttcgcc atcccgttcc tgccgcgcgg gcttgacgag ataccgcgct cccccaacga
     3661 ccgcgacgcc atcatcacgg tccgccgtag cggcctggtc aaatcgaact cggatgtcac
     3721 gctgccccaa ctgaacgccg actacgccga cacctacgtc aacggggtgc gctaccgcgt
     3781 gcggaccgtc gcgatacccg ggccggagcc gacgtcgctc gcggtgggcg cgacgtatga
     3841 caccaccctc gccgagacca acaatttgca ccgccgggtg ctgctgatct gcgggttcgc
     3901 tatcggcgcg gcggccgtgt tcgcctggct gctggccgca ttcgcggtac gtccgttcaa
     3961 acagctcgcc gagcagaccc gcgcgattga ggccggggac gaggcgccgc gggtcgaagt
     4021 gcacggcgcc agcgaagccg tcgagatcgc cgaggcgatg agaggcatgc tgcggcgcat
     4081 ctgggacgag cagaaccgga ccaaggaagc gctggcctcg gcgcgcgact tcgcggcggt
     4141 gtcgtcccat gagctgcgca ctccgctgac cgcgatgcgc accaatctcg aggtgctggc
     4201 cacactggac ctgcccgacg atgaacgcaa agaggtgctc aacgacgtca tccgcaccca
     4261 gtcgcggatc gaggccaccc tgagcgcctt ggagcggttg gcccagggcg aactgtctac
     4321 ggccgacgat cacatgccgg tcgacatcac cgaattgctc gaccgagcgg cgcacgacgc
     4381 catgcgggtc taccccgaac tcgacgtctc gctggtgccg tcgccgacct gcatcatcgt
     4441 gggattgccg gccgggttgc gactggcggt cgacaatgcg atcgccaacg ccgtcaagca
     4501 cggcggcgcc tctcgcatcc agctgtcggc ggtcagctcg cgcgcaggcg tggaaatcgc
     4561 catcgacgac aacggcgtcg gagtgcccga gacggagcgc caagtggtgt tcgagcggtt
     4621 ctcccgcggg tcgacggcct cgcattcggg gtcgggccta gggctggcat tggtggctca
     4681 gcaggcccag ctgcacggcg ggacggcgtc gctggaaaac agcccgctgg gcggcgcgcg
     4741 gctggtgttg cgactccccg ctccgtgcta gccgaactgc ctgacgatca ctttcgtgag
     4801 ctcttgcgcc acggcgtgaa aagcgcccgc gcggcggatc cctcgtcctt gaaccagacc
     4861 tgcgcgacgg tcgcgtcgta ggtcggatcg tcgggggtgt aatagagcct ggtatccgaa
     4921 cggcccttca ccaaccagcc cgacggcccg ccgccatccg gggtggcgcg agcggagccg
     4981 gggccgtacg gcgcatacgg caacacgggg atcttcgtgg tcggcgattc gtgggcgccg
     5041 ggacgcttct tcgccggccg cgccccgggg ggcggaatct tcttgcctgg cgcggccttg
     5101 gctgccggac cctttctggg cggcccgttt ctcggcggca gctttttcgc ggccgggatc
     5161 ttcctcgtcg gcgactcgcc gggcggcgga gcctttcttg gcggcacttt ccccggcggt
     5221 gctttccccg gcggcacctt cttgagcggc gccttcttgg gcggcgcctt cttcgggggc
     5281 gccttcttgg cagccgggat cttcttcgtc ggcggctcgc cggcggcagc atccctcttc
     5341 ggcagccatc gcttgatgaa cgggaacctg gtcgtgggcg aatctttcgc gaccggatgc
     5401 tcgatcttcg gcgactcttt ggcgacggca atctttgtcg tcggcgggtc gtgctccacc
     5461 gggatcttgg tcgtcggcgg ctcatgctcc accggaatca gggtcgtcgg cgattcattt
     5521 tcgacgggga tggtcgtcgt caaggactcg tcggcgacgg gaatctccgt cgtggccggc
     5581 tcggattcgg catcggcgac tggacccgtg gcagttgctg acacccgcgc cggcacttgg
     5641 cgtttgacgg gtcgaagcgt cagcgcgagg gtcaacacca accccgtcac aaaggccaac
     5701 ccgaagagcc accaatgcac gtggttcatc gctgcccgcc tgccccgggc ccggtggcat
     5761 cgccggggac gatttcagcg ccggcccgtt tgttcacgat attggcgatc acccgggcga
     5821 cggccgaacc cgccacgaag gcgagtaggt accacaacca ctggatcacg aaatccatcc
     5881 tggatcccct cagctcacaa cgatttcgac gcggcgattc ttggctcggc cctcgacggt
     5941 gtcgttgggg gcgaccgggt tgagtgaacc gagccccgtg accaccagct cagcggcggc
     6001 aacgccgtgg gcagtgagaa agtcggcgac ggtttgggct cgttggttgc tcaacggaat
     6061 gttgatggcc tcggtgccgg aattgtcggt gtagccgttg atcgtcgcgt gcgcgttcgg
     6121 gcatgccttg agcttgtctg ccacctgagt caatatctgc tcgtcggccg gggtcaggct
     6181 gaatccgtcg ttgccgaatg tgatcggccc accagtcgcg gcgttgatgg ccgattgcaa
     6241 gtcggcgcag ggcccggggg caggcggcgg gggcggtggg ggtggaggtg cgccgggctt
     6301 cgctggtggc ggcggcggat tcgcagcgtt aaccaccagc ctgtcaacga cattcagatt
     6361 tgaccaggtg tgcgctgtct cctgctcgac ggtgttcttc tggtcttgcg atgcggccgt
     6421 gcccgctaac gtaatggagt cgccgttgac cgtgagactg aaatcgggaa tcgaggcgct
     6481 gtctttgaaa attgctccgg cgttggagaa gtcgagcgcg tcgacattgg ggttgatgtg
     6541 gatttggtcg acgatgttga cgccgggagc cagcgagccg gtgagcgcct tcatcaacgc
     6601 cgctttagcg gagtcgtcgg gaaagtcacc ggtgagcgta atggtgttgc cgttgcgagt
     6661 gattgacagc ggcgccaggg aaaacttcgg ggcaccagac ttgctcgacg gtgacagggt
     6721 gggaaccgcg cccgtgggcc cgttgacgga ccgcggtcgc tcgtaggcac cgtagccgat
     6781 tgccgcaatc agcaaaggaa taaccacaac ggcgatcagc cagggacgcc caagcgatcg
     6841 ccagccagac tccggagctc gccgcgagtc ggtggttgcg gccgcttgcc gcagccccgc
     6901 ccctaaaccc actagtgccc ctctttcatt ccgttaccgg gactgatccg tcctgcaggc
     6961 gatatgcagc ctaccgagtc gatgccccga cgcccctcaa gtcgggcaca ctagagcgat
     7021 gggcaacggc aaaaaaccga ccgacagtga aaccctggca cacatccgcg ggctggtcgc
     7081 ggaggagaaa gcgctgcggg agcagctgca gca
//
LOCUS       DASSGQ010000059         6573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_95319, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000059 DASSGQ010000000
VERSION     DASSGQ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95319"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1240
                     /locus_tag="VFI55_02480"
     CDS             2..1240
                     /locus_tag="VFI55_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7072933.1"
                     /translation="MASLAVTAVTAVSTFNAYRPLVRRGQLSVFSWMLGLVVTEFPLQ
                     TLASQLGGLASTDRHLTRPVRIIAWLVSGISTLGLLNFSRAGHQANVPLTAALDNGLG
                     TSRRTDSVGLWRRPSGAGTAKSPGALRMMRIYRDYAHDSNISYGEYGSANHLDIWRRP
                     DLDRNGKAPVLFQIPGGAWTTGNKRGQAHPLMSHLAELGWICVAINYRHSPRNTWPDH
                     IVDVKRALAWVKAHIGEYGGDPDFIVITGGSAGGHLSALAALTPNDPQFQPGFEDADT
                     RVQAAVPLYGVYDFTRFDDAMHPMLPALLVKSVIKQRPSTNPQPFVTASPINHVSADA
                     PPFFVLHGSNDSLVPVEQARAFVERLRQLSRQPVVYAELPFTQHAFDILGSARAAHTA
                     VAVEQFLAEVYATQRQANVA"
     gene            complement(1237..1866)
                     /locus_tag="VFI55_02485"
     CDS             complement(1237..1866)
                     /locus_tag="VFI55_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7072934.1"
                     /translation="MSRERKGSEAVARRDSYHHGDLKRALTSAALSLVAEKGPKGFTL
                     TEAARRAGVSAAAPYRHFADKAELLAAVAEQGFCELHADLVAAVDAVPEPKARVIELG
                     RAYVRWAVTHPDRYQVMFGAESEKLAHPALAVAAERAFGDLRDAITRCQEGGIREGQD
                     PREIAAPLWSLVHGVASLAIGGELRAVGIHQDPEAMIADVVNRLLGDGG"
     gene            1927..2355
                     /locus_tag="VFI55_02490"
     CDS             1927..2355
                     /locus_tag="VFI55_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HET7072935.1"
                     /translation="MDKQLVTDRAGDRDREKSAARLGQALAQGYLQLDEYEQRVQAVF
                     QTHTKTELNQLLADLPLERIRRADPRRRAARIAAVQRGVRIHLAAFVAMAATALIVWA
                     AIAATTGASYFWPIWPILGAAIGLASHALAVRPSVKVIGR"
     gene            2547..4391
                     /locus_tag="VFI55_02495"
     CDS             2547..4391
                     /locus_tag="VFI55_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020725729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET7072936.1"
                     /translation="MTEGDPTVVRHRSGALVSTLPQAFQETAALHPDATAIRTVGARV
                     VITWSQYADRVRKIATGLSALGVERGDTVGLMLRNRPEFHLADTAAMHLGAIPFSIYN
                     TSAPEQIRYLFSNAENKIVITEAEFLPAIKAAGAELTRVVVIDADVDGCMTLEHLESL
                     EAGTDFDFESAWRGVGPDDVATLIYTSGTTGPPKGVELTHANLIAAFSAIVNLLGMQP
                     TDRITSYLPHAHIADRVSSHYANIVLGVEVTDVADTRAIVEALPDARPTVWVGVPRVW
                     NKIKAGIETKLGAEPNAIKRRLALWAIHTGIRAARLTLAGTSVPRSLAFRRNIADRLV
                     LAKLRHALGLEQVRWAVIGAAPSPVETIEFVWGLGVPVYEVWGLSESVGCGTSNRPGA
                     SKIGSVGQALSGVEISTAGDGELLMRGPVVMRGYRHQPEKTIEAIDLDGWLHSGDIGT
                     IDEQGFVTIVDRKKELLVNEAGKNLSPANIEMAIQSTSTLIDQVVALGDGRPYVTALV
                     VLDSDAVAAQAAALGMADTSLAAFVQSGAAREMLTAAVLEGNAKLSRVEQIKRFVVLG
                     TPWEPGGDELTPTMKLKRRPIAEKYSAEIDNLYANAPGPDVINLSGPG"
     gene            complement(4388..4867)
                     /locus_tag="VFI55_02500"
     CDS             complement(4388..4867)
                     /locus_tag="VFI55_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072937.1"
                     /translation="MFGFLRNWSEIGQLPAELREQLEAEGVLFAAGKVGVSRHFSGHV
                     PGVYSASGVSRYTGGFGFSTERVVATFPTRGDAKLRSIDCAWGTNKGPARASITAKGL
                     QIEIDLHGVDPAFSGSMRLNYKKKIPDDVLQELPATALRFRVEPVFVYRAAGVRPKA"
     gene            complement(4922..5356)
                     /locus_tag="VFI55_02505"
     CDS             complement(4922..5356)
                     /locus_tag="VFI55_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HET7072938.1"
                     /translation="MRIRLLVAVAVLVLSTMVGCTFASRNPPSTQTLEVPMDDVLKQS
                     VITQNVDLGVGNTLVIKLGSNYTTPYRWKPDTKIGDPTIMKQSGHEFVQPTSDARGAA
                     GTEVWTFTALKAGTTTISTSYTSFVGKNAKPACTYTANVTVQ"
     gene            5558..>6573
                     /locus_tag="VFI55_02510"
     CDS             5558..>6573
                     /locus_tag="VFI55_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HET7072939.1"
                     /translation="MFGLVLIVALVSTVIVGTILGRRYRVGPPVLLIVLGALLGLIPQ
                     FGGVRIDGEIVLLLFLPAILYWESMNTSFREIRANARVIVFLSIGLVIATSLAVAWTA
                     RAFGMESHAAAVLGAVLSPTDAAAVAGLAKQMPRRALTVLKGESIINDGTALVLYAVS
                     LSVAVGGAPVSPAALTGRFVISYVGGIAAGLLVGALVTLIRNYIDAPLEEGALSLLTP
                     FAAFLLAQSFDCSGVVAVLVSAFMLTYYGPKVIRARSRLLSFAFWDITTFLLNGSLWV
                     FVGVQIPSAVRGIDHVDGGIRHATVLALAVTAAVILSRLAWGEATTVVLRAVDRREVQ
                     RSRRV"
BASE COUNT         1137 a         2198 c         2087 g         1151 t
ORIGIN      
        1 aatagcgtca ctggcagtga cggcggtgac cgcggtttcc acgttcaacg cgtatcgacc
       61 gctggtgcgg cgcgggcagc tgtcggtgtt ctcgtggatg ttgggcctgg tcgtcaccga
      121 gttcccgctg cagacactgg ccagccagct cggcggactg gcgtcgacag accgccacct
      181 cacccgcccg gtgcgcatca tcgcgtggct ggtctcgggc atctcgacgc tgggtttgtt
      241 gaacttcagt cgggccggcc accaggccaa tgtgccgctg acggccgcgc tggacaacgg
      301 cctgggaacc tcacgccgta ccgattccgt gggtctgtgg cgccggccga gcggcgcggg
      361 caccgccaaa tcgccgggag cgctgcggat gatgcggatc taccgcgact acgcccacga
      421 ctcaaacatc agctacggcg aatacggcag cgccaaccac ctggacatct ggcgacgccc
      481 cgatctcgat cggaacggca aggcgccggt gctcttccag atccctggcg gtgcctggac
      541 gacgggaaac aaacgcggac aagcgcatcc gttgatgagc cacctcgccg agctgggctg
      601 gatctgcgtg gcgatcaact atcggcacag cccacgtaac acctggcccg accacatcgt
      661 cgacgtcaag cgcgccctcg cgtgggtcaa ggcgcacatc ggcgaatacg gcggcgatcc
      721 agacttcatc gtcattacgg gcggttcagc gggcggccat ctgtcggcgc tggcggcgtt
      781 gacgcccaac gacccgcagt tccagccggg attcgaagac gccgacaccc gggtgcaagc
      841 ggccgtaccg ctctatggcg tctatgactt cacccgtttc gacgacgcga tgcatcccat
      901 gctgccggcg ctgctcgtga aatcggttat caagcaacgg ccctcgacca acccgcagcc
      961 gtttgtcacc gcatcgccga tcaaccacgt gtcggccgac gctcccccat tcttcgtcct
     1021 gcacggcagc aacgactcgc tggttcccgt cgagcaggcg cgcgcgttcg tcgaacggct
     1081 ccggcagcta agcaggcagc cggtcgtcta cgccgaattg cccttcacgc aacacgcttt
     1141 cgacatcctc ggttcggcgc gcgcggcgca caccgcggtc gccgtcgagc agttcctggc
     1201 cgaggtttac gccacgcagc gccaggcgaa cgtggcctaa ccgccatctc caagcagtcg
     1261 gttcaccacg tccgcaatca tggcctccgg gtcttggtgg atgccgacag cacgtagttc
     1321 gccaccgatg gcgagcgaag cgacgccgtg caccagcgac cagagcggcg cggcaatctc
     1381 gcgggggtct tgaccctcgc gaattccccc ctcctgacac ctggtgatcg cgtcgcgcag
     1441 gtcaccaaac gcccgttccg cggcgaccgc cagggcaggg tgagccaatt tttcgctctc
     1501 ggcaccgaac atgacctgat agcggtcggg atgggtgacc gcccagcgca cataagcgcg
     1561 gccgagttcg atcaccctgg ccttcggctc cggtaccgca tcgacagccg cgaccaggtc
     1621 ggcgtgcagt tcgcagaatc cctgttcggc gacggcggcc agcagctcgg ccttgtcggc
     1681 gaagtgccgg tacggcgccg cggcgctcac gccggcgcgc cgtgccgcct cggtaagggt
     1741 gaagcctttc ggccctttct cggccaccag ggacagcgcg gcgctggtca gcgcacgttt
     1801 gagatcgcca tggtggtaac tgtcacgacg cgccacggcc tccgatccct tccgctcacg
     1861 tgacatgttg acgacattaa cattatcgtc tatgttaatg ctattcacat cccggaagga
     1921 acggcaatgg acaagcaact cgtgaccgac cgcgccggag accgcgatcg cgagaagagc
     1981 gccgcgcgcc tgggccaagc tctcgcccag ggctacctgc aactcgacga gtacgagcag
     2041 cgggtccaag cggtgttcca aacccacacc aagacagagc tcaaccaact cctcgccgat
     2101 ctgccgctgg agcgcatccg gcgcgcggac cctcgccgcc gcgccgcgcg gatcgccgcg
     2161 gttcaacgcg gggtgcggat tcatctcgcg gccttcgtcg cgatggccgc cacggcgctc
     2221 atcgtctggg cagcgatcgc cgccaccacg ggcgcgtcat atttctggcc gatatggccg
     2281 atcctcggcg ccgcgatcgg cctcgccagt cacgccttgg cggtgaggcc gagcgtgaaa
     2341 gtaattggca gataacgatt ttagaaggat cagcaatgtc gaaacaccga gcacgggcgg
     2401 acgactggca cgcgcttggc tcgcggcgct cgtcaccggg cggacgccag ggcccgccga
     2461 ggtggcctga gcggtaacgt gccatagacc ggcgccgata cgggattaag gaagagtctg
     2521 cggtgacggc acgggaagtg ggaacaatga ccgaaggcga cccaacggtg gttcggcatc
     2581 gcagtggagc actggtgtcc acgctgccac aggcttttca ggagaccgcg gccttgcacc
     2641 cggacgcgac ggccatccgg acggtcggcg ccagagtggt catcacgtgg agccagtacg
     2701 ccgatcgcgt gcggaagatc gccaccgggc tgtcggcgct tggcgtcgaa cgcggcgaca
     2761 ccgtaggtct catgctgcgc aaccgtcccg aattccactt ggccgacacc gccgccatgc
     2821 atctcggcgc gatcccgttc tcgatctaca acacctcggc gcccgaacag atccggtacc
     2881 tgttcagcaa cgccgagaac aagatcgtca tcaccgaagc ggaattcctg ccggcgatca
     2941 aggcagccgg ggcggagctt acccgggtgg tcgtcatcga cgccgacgtc gacggctgca
     3001 tgacgctcga acatctcgaa tccttggaag ctggaacaga tttcgatttc gagtcggcat
     3061 ggcgaggggt agggccagat gacgtcgcga cgcttatcta cacgtcggga accaccgggc
     3121 cgcccaaggg cgtcgagctc acgcacgcca atttgatagc cgcgttctcg gcaatcgtca
     3181 atctccttgg catgcaaccg actgaccgga tcacctcata cctgccgcac gcgcacatcg
     3241 ccgatcgcgt gtcctcgcac tacgccaaca tcgtcctggg cgtggaggtg accgacgtcg
     3301 ccgacaccag ggcgatcgtg gaggcgctgc ccgatgctcg ccccaccgtg tgggtcggag
     3361 tgcctcgcgt gtggaacaag atcaaggccg ggatcgaaac gaagctgggc gcggagccca
     3421 acgcgatcaa gcgacggtta gcgctgtggg ccatccacac cggtatccgg gccgcccgcc
     3481 tgacactggc aggcacgtcc gtgccccggt cacttgcctt ccgccgcaac atcgccgacc
     3541 gcctggtgct ggccaagttg cgccacgcac tcggcctgga acaggtgcga tgggcggtga
     3601 tcggcgcggc accgagtccg gtcgaaacga tcgaattcgt gtggggactg ggcgttcccg
     3661 tctacgaggt ctggggattg tcggagagtg tgggctgtgg gacctccaac cgacccggcg
     3721 caagcaagat cggttcggtc gggcaggcgc tttccggcgt cgagatctcg acggccggcg
     3781 acggcgaact gctgatgcgc gggccggtcg tgatgcgggg ctaccggcat cagccggaga
     3841 agaccatcga agcaatcgac ctggacggct ggttgcacag cggtgacatc ggcaccatcg
     3901 acgaacaggg tttcgtcacg atcgtcgacc gcaagaagga gttgctggtc aacgaagccg
     3961 gaaagaatct ctcccccgcc aacatcgaga tggcgatcca gtccacgtcc acgttgatcg
     4021 accaggtagt cgcgctcggc gacggcaggc cgtacgtgac ggcgctcgtc gtcctggaca
     4081 gtgatgccgt cgccgcacag gccgccgcgc tcggcatggc cgatacgtcg ctggccgcct
     4141 tcgtgcaaag cggcgcggcg agggaaatgc tcaccgcggc cgtcctcgag ggcaacgcca
     4201 aactttcccg cgtcgagcag atcaagcgct tcgtggtcct cggcacgccg tgggagccgg
     4261 gcggagatga gctcacgccg acgatgaaac tcaaacgtag gcctatcgcc gaaaaatact
     4321 ctgcggagat cgacaatctc tacgccaacg cgccggggcc ggacgtcatc aacttgtccg
     4381 gcccgggcta ggccttggga cgcacgccgg ccgcgcgata cacgaacacc ggctccaccc
     4441 gaaaccgcaa cgccgtcgcg ggcagctctt gcagcacgtc gtccgggatc ttcttcttgt
     4501 agttcagcct catcgagccg gagaaggcgg gatcgacccc gtgcaggtcg atctcgatct
     4561 gcaggccctt tgctgtgatg cttgcccgcg ccggcccctt gttggtgccc caagcgcaat
     4621 cgatggaccg cagcttggcg tcaccccgcg tggggaaggt ggcgacgacc cgctcagtgc
     4681 tgaagccgaa cccgcccgtg tagcgggaga cgcccgaagc cgaatagacg cccggcacat
     4741 ggccgctgaa gtgccggctt accccgactt tgcccgcggc gaagagcacg ccctcggcct
     4801 cgagttgttc gcgcagctct gcgggcagtt ggccaatttc ggaccagttg cgaaggaagc
     4861 cgaacacagc cagacggtag cccggtaggc cacccgcgcg cagggatttg ccagagtcgt
     4921 tttactgcac cgtcacgttc gccgtgtagg tgcacgcggg cttggcgttc ttgcccacga
     4981 agctggtgta ggaggtgctg atggtcgtcg tcccggcttt caaagcagtg aaggtccaca
     5041 cctcggtgcc ggcggcgccc cgcgcatcag aggtcggctg cacaaattcg tggccgcttt
     5101 gcttcatgat cgtcggatcg ccgatctttg tatcgggctt ccatcggtac ggggtggtgt
     5161 agttcgagcc caacttgatg acgagcgtgt tgccgacgcc caggtcgacg ttctgggtga
     5221 tgacgctctg tttcagcacg tcgtccatgg ggacctcgag cgtttgcgtc gacggcgggt
     5281 tcctggacgc gaacgtgcag cccaccatcg tcgacaaaac gagcacggcg actgcgacca
     5341 gcagtctgat cctcacgaaa atcccccttc aaattccgct ccgcagccta gtgttcaggt
     5401 cgccattccc gccgcaggtc agcgttaatc gactcaatcg gatgaacacg agccggccgg
     5461 tcaaaccggc tccatcccat tgtcaccgcg tcgccaggga aggatcggca gcaaaccccg
     5521 cgcggcgcgc gccgagccgg tccggcagga tgggctggtg tttggccttg tcctcatcgt
     5581 tgctctcgtc tccaccgtca tcgtgggaac gatcctgggc cggcgctatc gggtgggccc
     5641 tcccgttctg ctcatcgtgc ttggcgccct actgggtctg attccccaat tcggcggagt
     5701 ccgcatcgac ggcgagatcg tcttgctgct gttcctgccc gcgatcctct attgggagag
     5761 catgaacacc agcttccgcg aaatccgggc gaacgcacgc gtcatcgttt tcctcagcat
     5821 cggcctggtg atcgccacct cgctggcggt cgcgtggacg gcacgagcgt tcggaatgga
     5881 atcgcatgcc gcggcagtcc tcggcgccgt gctgtccccc accgacgccg ccgccgtggc
     5941 cggcctggcg aagcagatgc cgcgcagagc gctcacggtg ctgaaaggcg agagcatcat
     6001 caacgacggc accgcgctcg tcctctacgc cgtcagtctt tctgttgcgg tcggcggcgc
     6061 cccggtcagc ccggcagcgt tgaccggccg ctttgtcatc tcctacgtcg gcggtatcgc
     6121 cgcgggattg ctggtcggcg cactggtgac cttgattcgc aattacatcg acgcgccact
     6181 ggaagaaggg gccctgagtt tgctgacgcc gttcgcggcg ttcctgttgg cccaaagctt
     6241 cgattgcagc ggcgtcgtcg cggtgttggt gtccgccttc atgctcactt attacggtcc
     6301 gaaggtgatc cgggctcgct cccgactcct gtcattcgcg ttctgggaca tcacgacgtt
     6361 tctgctcaac ggctcattgt gggtgtttgt cggagtccag atccccagcg cggtgcgggg
     6421 tattgatcac gtcgacggcg gaattcgcca cgccacagtc ctggccctcg cggttactgc
     6481 cgcagtcatc ttgtcccggc tcgcttgggg cgaagccacc actgtggtgc ttcgtgccgt
     6541 cgaccggcgc gaggtgcagc gctcccgccg agt
//
LOCUS       DASSGQ010000060        13129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1431979,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000060 DASSGQ010000000
VERSION     DASSGQ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13129
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431979"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2547
                     /locus_tag="VFI55_02515"
     CDS             <1..2547
                     /locus_tag="VFI55_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019731102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HET7072940.1"
                     /translation="DTGTPQFEIVDPQPLTLLPEPDPGDLFFDFEGDPLWTADGRDWG
                     LEYLFGVLEARGDFHPLWAHHRVDERRALTDFLALVAKRRKRRPNMHIYHYAPYEKTA
                     LLRLAGRYGVGEDEVDELLRSGTLVDLYPLVRKSIRVGAESFSLKALEPLYMGPQLRV
                     GDVTTAAESITSYARYCELQAAGRSDEAASVLKEIEDYNHYDCRSTRGLRNWLMLRAY
                     ESGVVPVGAQPVRQGNTVEDRDQLATTLSTFAGDAGIVDRTPEQTAVALVAAARGYHR
                     REDKPFWWAHFDRLNFPVDEWADNTDVFVAADASVSVDWHTPPRARKPQRRVRLRGEL
                     ARGDLMTNVFALYEPPAPPSMTDNPDRRAAGRAVVVDADDPTLPSEVVIVERVGNDGN
                     TFHQLPFALTPGPPVPTTALRESIESSSVTVAAGLPHLPHTAALDILLRRAPRTRSGG
                     PLPRSGDTVTDITAAALDLDSSYLAVHGPPGTGKTHTAARVIMQLVTGHDWRIGVVAQ
                     SHATVENLLDCVIDAGLDPGRVAKKRYDHAAPRWQEIDGSEYATFIGNNAGCVIGGTA
                     WDFANANRVTPGSLDLLVIDEAGQFCLANALAVAPAAANLLLLGDPHQLPQVSQGTHP
                     EPVDASALEWLLDGQRTLPGERGYFLDRSYRMHPAVCAAVSALSYECRLHPHTERTAA
                     RHLNGYQPGLHVIPVAHHGNSTESPEEADAIAAEIGRLLGTAWTDERGTRPLAASDVL
                     VLAPYNAQVALVRQRLGSVGLAGVRVGTVDKFQGAQAPVVFVSMTASSVDVVPRGISF
                     LLNRNRLNVAVSRAQYAAVIVRSQVLTEYLPATPAGLIDLGAFLALTEPV"
     gene            complement(2570..4633)
                     /locus_tag="VFI55_02520"
     CDS             complement(2570..4633)
                     /locus_tag="VFI55_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET7072941.1"
                     /translation="MTTATPRSPGGTRRAGPRRADFANRWRSIAGSNPSQSANPWNAL
                     WAMMIGFFMIMVDSTIVAIANPTIMADLRIGYGAVVWVTSAYLLGYAVVLLVAGRLGD
                     RFGPKNLYLIGLVVFTAASMWCGLSGSAAMLIAARVVQGVGAGVLTPQTLSTITRIFP
                     AHRRGVAMSVWGATAGVASLVGPLAGGALVEGLGWPWIFFVNVPIGIVGLALAVRLVP
                     VLPTQAHRFDLIGVALSGVGMFLIVFGLQQGQSAHWQPWIWAMIVAGIGLMTAFVYWQ
                     SLNTREPLVPLQIFGDRDFSLCNVGVGIISFAATAMMLPLTFYAQAVCGLSPTRSALL
                     IAPMAVVGGVLAPLVGKIVDRYHPGPLLGFGFSVLAIALTWLAIEMSPDTPIWRLVLP
                     FSAIGVGMAFVWSPLTATATRNLPPHLAGAGSAAYNSIRQLGAVLGSAAMAAFMTSRI
                     SAEVPRSPGTATGDGAHALQLPEFLRAPFSAAMSQSVLLPAFVTLLGIVAALFLVGFG
                     PLVVGRKGTGTSSSMPDEGDVDDAGYDDDEYVEFILLREPDAGPPPRADVEPPSEKDR
                     GTVPVPERFAHPRPTSTGSGHNDRAGPWRGPVDHPIPRAAPIGFAHNGSHVDTGKRLR
                     RAASPNAFGPAAPDDRRFAPPQEWHHNGSAHRQFTDGAPPRGPRYRPDPDEDPSDYGR
                     HSSGK"
     gene            4944..5681
                     /locus_tag="VFI55_02525"
     CDS             4944..5681
                     /locus_tag="VFI55_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072942.1"
                     /translation="MSNPGDAPNGTGGRPKASTRALAQVIERSSRLHGPAAEAYVARL
                     RRTHPGASPAEIDAKLETRYLAALTASGAAVGSAATFPGIGTLTAMSAGAGETIFFIE
                     ATATFVLAKAVVYNVPADHRERRRALVLAVLVGDDSRRALGEVIGPARTNGGWLAEGM
                     ASLPLPALARFNARMLKYFIKRYTMRRGALMFGKMLPVGIGAVVGGAGNRMAGKKIVL
                     NAREAFGAPPTRWPGTLRLLPPTQEVG"
     gene            5886..9581
                     /locus_tag="VFI55_02530"
     CDS             5886..9581
                     /locus_tag="VFI55_02530"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HET7072943.1"
                     /translation="MSNISSPFGQNEWLVEEMYRKFRDDPSSVDPSWHEFLVDYNPEP
                     ITESAPTAADKPSAPQATPAPVPSAAAAAPSTTAAGGNGAAVPTAPPPAQPSGPPPAE
                     DDELQVLRGAAAAVVKNMSASLHVPTATSVRAVPAKLLIDNRTVINNQLKRTRGGKIS
                     FTHVLGYALVQAVKAYPNMNRHYAEVNGKPNAVTPAHTNLGIAIDLHGKDGKRSLVVA
                     GIKRCEELRFAEFVSAYEDIVRRARDGKLTAEDFAGVTISLTNPGTIGTVHSVPRLMA
                     GQGAIIGVGAMEYPAEFQGASAERIAELGIGKLITLTSTYDHRIIQGAESGDFLRTIH
                     EMLLSDDFWDEIFRELGNPYLPVRWSTDNPDSIVDKNARVMELIAAYRNRGHLMADVD
                     PLRLDQTRFRSHPDLEVLTHGLTLWDLDRVFKVNGFAGCEYKKLRDVLGLLRDAYCRH
                     IGVEYTHILEPEQQKWLEQRVETKHVKPTVAEQKYILSKLNAAEAFETFLQTKYVGQK
                     RFSLEGAESQIPLMDAAIDQCAEHGLDEVVIGMPHRGRLNVLANIVGKPYSQIFSEFE
                     GNLNPSQAHGSGDVKYHLGATGVYLQMFGDNDIQVSLTANPSHLEAVDPVLEGLVRAK
                     QDLLDPGDDGETKAFSVVPMMLHGDAAFAGQGVVAETLNLANLPGYRVGGTIHIIVNN
                     QIGFTTAPEYSRSSEYCTDVAKMVGAPIFHVNGDDPEACVWVAKLAVDFRQKFNKDVI
                     IDMLCYRRRGHNEGDDPSMTNPYMYDVVDTKRGARKSYTESLIGRGDISLKEAEDALR
                     DYQGQLERVFNEVRELEKHGAQPSLSVEEDQMIPAGLDTAVDKSLLARIGDAFLALPD
                     GFTVHPRVLPVLERRREMAYEGKIDWAFGELLALGSLVAEGKWVRFSGQDTKRGTFSQ
                     RHSVIIDRSTGEEFTPLQLLATNPDGRSTGGKFLVYDSPLSEYATVGFEYGYTVGNPD
                     ALVLWEGQFGDFVNGAQSIIDEFISSGEAKWGQLSNVVLLLPHGHEGQGPDHTSGRIE
                     RFLQLWAEGSMTIAVPSTPANYFHLLRRHALDGIARPLIVFTPKSMLRNKAAVSDIKD
                     FTEIKFRSVLEEATYEDGIGDRSKVRRILLTSGKLYYELAARKAKDKRDDIAIVRIEQ
                     LAPVPKRRLSETLDRYPNAGEFFWVQEEPANQGAWPRFGLELPELLPDKLTGIKRVSR
                     RAMSAPSSGSSKVHAVEQQEIIDTAFG"
     gene            9773..10603
                     /locus_tag="VFI55_02535"
     CDS             9773..10603
                     /locus_tag="VFI55_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7072944.1"
                     /translation="MRGFAGKVAAVTGAGSGIGQALAVELARSGAKLAISDVDTEGLA
                     QTEEQLKAIGAPVKAGRLDVTEREAFLAYADAVNDHFGQVNQIYNNAGIAFTGDIEVS
                     QFKDIERVMDVDFWGVVNGTKAFLPYLIASGDGHVINVSSVFGLFSVPGQAAYNSAKF
                     AVRGFTEALRQEMVLAGHPVGVTTVHPGGIKTAIARNATAAEGLNRDELAKLFDKRLA
                     KTSPQRAAEIILEAVDKNKARVLVGTDAKLLDLMVRLSGSGYQRIFGSVLGRLGLSKH
                     "
     gene            complement(10616..11488)
                     /locus_tag="VFI55_02540"
     CDS             complement(10616..11488)
                     /locus_tag="VFI55_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7072945.1"
                     /translation="MRIGKLAAALLAATLAVVLWAGCTTHTGDHPSEVVVGSRPDSES
                     MLLAAIYVAALQSYGFAARADTAEDPMAKLDSGEFTVLPAFTGQTLRRLQPGAFALSD
                     AQVYRAMIAALPEGILAGDYTTAAADKPLLLVSQATASAWAGHEPSADLSTLPSHCDG
                     LTIGAVTGFEEPSVVGSCRLPAPREFADQATLFAAVRNGQPTAGWTSTADPGIPADLT
                     ALPDGKPALIQAENVVPLYRRNALSDRQLLAVNEVAGVLDTAALAEMRRQLAGGAGPQ
                     AVAGGWLAEHPLGR"
     gene            complement(11485..12675)
                     /locus_tag="VFI55_02545"
     CDS             complement(11485..12675)
                     /locus_tag="VFI55_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HET7072946.1"
                     /translation="MPESVLPQIVIGNDEIFDAHVGGKLLVELKSPLDTQRALSIAYT
                     PGVAQVSRAIAADRTLAARYTWANRLVAVVTDGSAVLGLGDIGPEASLPVMEGKCALF
                     QAYGGLNAIPIVLATKDPDEIVETLVRLRPTFGAVNLEDISAPRCFEIERRVIEALDC
                     PVMHDDQHGTAIVVLAALMGATKLLGRDMSSVRVVVSGAGAAGVACTNLLLAMGVSHI
                     TVLDSRGILHTGRNDMNSVKVDLARRTNPSSLSGGMSEALKGADVFLGVSAGVVPEEL
                     IATMAPDGIVFALSNPDPEIHPHIAAKYAAVVATGRSDFPNQINNVLAFPGLFRGALD
                     AGARRITEKMMVAAAEAIFAVVSDDLAVDRIVPSPLDLRVGEAVASAVALAAEVSDAA
                     VSWE"
     gene            complement(12733..>13129)
                     /locus_tag="VFI55_02550"
     CDS             complement(12733..>13129)
                     /locus_tag="VFI55_02550"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215756.1"
                     /note="catalyzes the oxidation of malate to oxaloacetate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET7072947.1"
                     /translation="AEVGGKNAAEVVGDQAWIEKDFIPTVAKRGAAIIDARGASSAAS
                     AASATVDAARDWLLGSPADNWVSMAVVSDGSYGVPEGLISSFPVTTKDGNWEIVGGLK
                     IDEFSRGRIDASTAELADERNAVTGLGLI"
BASE COUNT         2368 a         4433 c         4385 g         1943 t
ORIGIN      
        1 gataccggga cgccacaatt cgagatcgtc gatccgcagc cgctgacact gttgcccgaa
       61 cccgaccctg gagacctgtt cttcgacttc gagggcgacc cgctatggac cgccgacggc
      121 cgcgattggg gcctggaata cctattcgga gtcctcgaag ccagggggga cttccacccg
      181 ctgtgggcgc accaccgggt cgacgaacgc agggcactca ccgatttcct tgcgctggtg
      241 gccaagcgcc gcaagcgccg tcccaacatg cacatctacc actacgcgcc ctacgaaaag
      301 accgcgttgt tacggcttgc gggacgatac ggagtcggcg aggacgaggt cgacgaattg
      361 ctacgcagcg ggacactcgt cgacctgtat ccgttggtgc gcaagagcat tcgcgtgggt
      421 gccgagtcgt tcagcctcaa ggccctcgag ccgctttaca tgggccccca gctgcgtgtc
      481 ggcgacgtca ccacggccgc cgagtcgatc acctcctacg cccgttactg cgaattgcag
      541 gccgccggcc gctctgacga ggccgcatcc gtactcaagg agatcgagga ctacaaccac
      601 tacgactgcc ggtcgacgcg ggggctgcgt aactggctga tgctgcgcgc ctacgaatcc
      661 ggagtcgtgc ccgtcggcgc gcaaccggtt cggcaaggca acaccgtcga agaccgtgac
      721 cagctggcga ccacactttc cacgttcgcc ggcgatgccg gcatcgttga ccgcacgccc
      781 gaacagaccg cggtggcgct ggtcgccgcg gcacgcggct atcaccggcg cgaggacaaa
      841 ccgttttggt gggcgcattt cgacaggctg aacttccccg tcgacgagtg ggccgacaac
      901 accgacgtct tcgtcgcggc tgacgcgtcg gtcagcgtcg actggcacac cccgccccgc
      961 gcccgcaaac cgcagcgacg agtgcggctg cggggagagc tggcacgcgg agacctgatg
     1021 acgaacgtct ttgcgctcta cgagccgccg gctccaccga gcatgaccga caatcccgac
     1081 cggcgggccg ccggacgggc cgtggttgtc gacgccgacg acccgacgtt gcccagcgag
     1141 gtggtgatcg tcgagcgggt tggcaatgat ggcaacacat ttcaccagct gccgttcgcg
     1201 ctcactcccg ggccgccggt tccgacaacg gcgttgcggg aatcgatcga atcgtcctcc
     1261 gtcaccgtgg cagccgggtt gccgcacctg ccgcacaccg ccgcgctcga catcctgctg
     1321 cgccgcgcgc cccgtacgcg cagcggcggc ccactgccgc gcagcggcga caccgtcacc
     1381 gacatcaccg cggcggccct ggacctggac tcgtcgtacc tggcggtgca cggaccgccg
     1441 gggaccggta agacgcacac ggctgcccgg gtgatcatgc agctggtcac cggccatgac
     1501 tggcgtatcg gtgtcgtggc gcaatcacac gccacggtcg agaacctgtt ggactgtgtg
     1561 atcgacgccg gcctggaccc cggccgggtc gccaagaaac gctacgacca cgccgccccg
     1621 cgatggcagg agatcgacgg cagcgagtat gccaccttca tcggcaacaa tgcgggctgc
     1681 gtgatcggcg ggacggcctg ggatttcgcg aacgccaacc gggtgacccc gggcagcctg
     1741 gatctgctgg tgatcgacga ggcgggccag ttctgtttgg ccaacgccct cgcagtggca
     1801 ccggcggccg ccaatctcct gctgctcggc gatccccacc agctgccgca agtcagtcag
     1861 ggaacgcatc ccgaaccggt cgacgcgtcc gcgctggaat ggttgctgga cggtcagcgg
     1921 acgctgcccg gcgaacgcgg ctacttcctg gaccgctcgt accgaatgca tcctgcggtc
     1981 tgcgcggcgg tctccgcgct gtcctatgag tgccgactgc atccgcacac tgaacgcacc
     2041 gcggctcgcc acctcaacgg gtaccagccc ggcctgcacg tgattcccgt cgcccatcac
     2101 ggcaactcga cggagagtcc agaagaggcc gacgcgatcg ccgccgagat tgggcggctg
     2161 ctcggcaccg cgtggaccga tgagcgcggc acccgaccgc tcgccgcctc cgatgtgctg
     2221 gtgctggcgc cctacaacgc ccaggtggcg ctggtgcggc agcggttggg ctccgtcggg
     2281 ctcgcagggg tgcgggtcgg aaccgtcgac aagttccagg gcgcacaggc gccggtggtc
     2341 ttcgtctcga tgacggcctc ctccgtcgat gtggtaccgc gcggaatctc attcctgctc
     2401 aacaggaacc ggctcaacgt cgcggtcagc cgggcacagt acgcggcggt gatcgtgcgc
     2461 tcgcaggtgc tgaccgagta cctacccgcc acgcccgccg gcctcatcga cttgggcgcg
     2521 tttctggcgc tgacagaacc tgtgtgaggc ccaaggggca agccgcggac tatttgccgg
     2581 aggagtgccg gccgtagtcg ctggggtcct cgtccggatc gggacggtac cgcgggcctc
     2641 ggggcggcgc gccgtcggtg aactggcggt gcgcggagcc gttatggtgc cattcctgcg
     2701 gcggcgcaaa ccggcgatcg tcgggcgcgg cggggccgaa cgcgtttggc gacgctgcgc
     2761 ggcgaagccg cttcccagtg tccacgtgcg agccgttgtg ggcgaagcca atcggcgcgg
     2821 cccgcggaat cggatgatca accgggccgc gccagggtcc ggcgcggtcg ttatgcccgc
     2881 tacccgtgga cgtcgggcgg gggtgggcga accgctccgg gacgggcacg gtgccgcggt
     2941 ccttctcgga cggcggttcc acgtccgcac gcggtggggg cccggcgtcc ggttcccgaa
     3001 gaagtatgaa ctcgacgtac tcgtcgtcgt catagccggc gtcatccacg tcgccctcgt
     3061 cgggcatcga tgagctggtg ccagtgccct tgcggccgac caccaaaggc ccgaagccga
     3121 ccaaaaacag ggcggcaacg atcccgagca acgtgacgaa cgcgggcaac agcaccgact
     3181 gcgacatcgc ggcggagaac ggcgcgcgca agaactcggg cagttgcaag gcgtgggcgc
     3241 cgtcgccagt ggccgtaccg ggcgaccgcg gcacctccgc gctgatccgc gatgtcatga
     3301 acgcggccat tgcggcgctg ccgagcaccg ctccgagctg gcggatcgag ttgtaggcgg
     3361 cagagcccgc gccggccagg tgcgggggca ggttgcgggt ggcggtggcc gtcagcggcg
     3421 accacacgaa cgccatcccg acgcctatcg cggaaaacgg caggaccagc cgccagatcg
     3481 gggtgtccgg ggacatctcg atcgcgagcc aggtcagcgc gatcgccagt accgaaaagc
     3541 cgaagccgag caggggcccc ggatggtatc ggtcgacgat cttgccgacg agcggcgcga
     3601 gcacaccgcc gacgaccgcc atcggcgcga tcaataaggc cgagcgcgtc ggcgagaggc
     3661 cgcataccgc ctgcgcataa aacgtcagcg gcagcatcat cgccgtcgcc gcgaacgaga
     3721 tgatgcccac cccgacattg cacaggctga agtcccggtc accgaagatc tgcaggggaa
     3781 ccagcggctc acgcgtgttc agcgactgcc agtaaacgaa cgccgtcatc aaaccgatac
     3841 cggcgacgat catcgcccag atccagggct gccagtgcgc ggactggccc tgttgcaggc
     3901 cgaagacgat cagaaacatg cccaccccgg acaacgcgac accgatcagg tcgaaccggt
     3961 gcgcctgagt gggcagcacc ggaaccagcc gaacggccag cgccagcccg acgatgccga
     4021 tgggaacatt aacgaagaaa atccagggcc agcccaaccc ctcgaccagc gcgccgccgg
     4081 ccaacggccc gaccaggctg gccacgccgg cggtggcacc ccacacgctc atcgcgacac
     4141 cgcgccggtg cgcggggaag atccgggtga tcgtcgacaa ggtctgcggg gtgagcaccc
     4201 cggcgccgac accttgcacg actcgagccg cgatcagcat cgcggcgctg cccgacagcc
     4261 cacaccacat cgaggcagcg gtgaacacca ccagaccgat caggtagagg ttcttggggc
     4321 cgaaccggtc acccagccgc cccgccacga gcaacaccac cgcgtatccc agcaggtagg
     4381 cgctggtcac ccagaccacc gcgccgtacc cgatacgcaa gtcggccatg atggtcgggt
     4441 tggcgatcgc gacgatggtc gagtcgacca tgatcatgaa gaagccgatc atcatcgccc
     4501 acagcgcgtt ccacgggttc gcggactgcg acgggttaga cccggcgatc gacctccaac
     4561 ggttcgcaaa atcggcgcgc cgcggccccg cgcgccgggt acccccggga gagcggggcg
     4621 ttgccgtggt catgtgcgca cctcgtttca gctcccgatg acggcggccg gtcactcggc
     4681 cctatgtggt caaccggctt acccgcacat caaaggtgat acgcgtggac gcggcaaagc
     4741 gcccaccacc aggaccggcg aacggcgccg acctgtttcc agcctgcatt attccgggtg
     4801 ccgcgattgg ctgcgcaccc gccgacccat aacgccgatg gctccgcagg tctccagcgc
     4861 agtccgcgtt ttgcgcgggc gagttagcct gaaaagacgc cataagcaag agaggcaaca
     4921 tgcgggcccg aggaaacaaa gatatgtcca atccaggaga cgcgccgaac ggcacgggcg
     4981 gccggcccaa ggcatccact cgggcattgg cgcaggtcat cgagcgcagc tcccggctac
     5041 acggaccggc cgcggaggcc tacgtggctc ggttgcggcg gacacacccg ggcgccagcc
     5101 cggctgagat cgacgccaag ctggaaacgc gatacctggc cgcgctcacg gccagcggtg
     5161 ccgcggtggg ctcggccgcg acattcccgg gcatcgggac gctgacggcg atgtccgccg
     5221 gcgccgggga aaccatcttc ttcatcgagg ccaccgccac gttcgtgctc gccaaggccg
     5281 tggtgtacaa cgtcccggcc gaccatcggg agcggcgccg cgccctggtg cttgccgtcc
     5341 tggtcggaga cgacagcagg cgcgccctcg gcgaagtaat cggtccggcc cgcaccaatg
     5401 gtggctggct ggccgaaggg atggcgtcgc tgccgctgcc tgccctggcg cggttcaacg
     5461 ccaggatgct caagtacttc atcaagcggt acaccatgcg gcgcggtgcc ctgatgttcg
     5521 gcaagatgct gccggtcggc atcggcgcgg tggtcggcgg cgccggcaac cgaatggcgg
     5581 gcaaaaagat cgtcctcaac gcgcgggagg cgttcggggc tccgccgacc cggtggccgg
     5641 gcaccctgcg cctgctcccc ccgacccagg aagtcggcta gcggcgaacg gcctaagtcc
     5701 cctccgtcag atacgcgcga attgccgcga attgctggcg aaaggttagc ctttatgagg
     5761 cggaaacctg gaagactgcc gagggtgaga gatgccccaa ccgaccgggg cgcacaaagt
     5821 cgcgggcgcc gcgcaatagc gcttaaagcg cctgcaggac aaaagaattg aggcgaacag
     5881 cggccgtgag caacataagt tcaccattcg ggcaaaacga atggctggtc gaggagatgt
     5941 accggaagtt ccgcgatgac ccgtcgtcgg tcgatccgag ctggcatgag ttcctggttg
     6001 actacaaccc cgagccaatc accgaatctg cgccgaccgc cgccgacaag ccgtccgctc
     6061 cgcaagcgac gcccgcgccc gtgccgtccg ctgcggccgc tgcgccctcc accaccgccg
     6121 cgggcggcaa cggcgccgcg gtgccgaccg cgccgccgcc ggcacaaccc agcggtccgc
     6181 cgccagccga agatgacgag ttgcaggtgc tgcgcggcgc ggccgcggcc gtcgtgaaga
     6241 acatgtccgc gtcgctgcac gtgccgacgg caaccagcgt ccgcgccgtt ccggccaagc
     6301 tgctgatcga caaccgaacc gtcatcaaca accagctgaa gcgcacccgc ggcggcaaga
     6361 tctccttcac ccacgtcctg ggctacgcgc tggtgcaggc ggtcaaggcg taccccaaca
     6421 tgaaccggca ttacgccgag gtcaacggga agccgaatgc cgtcacaccg gcccatacca
     6481 atctaggcat cgcgatcgac ctgcacggca aagacggcaa gcgctcgctg gtggtggccg
     6541 gcatcaagcg ctgcgaggaa ttgcgattcg ccgagttcgt ctcagcctac gaagacatcg
     6601 tccggcgggc gcgcgacggc aagctgaccg cggaagattt tgccggcgtg acgatttcac
     6661 tgaccaaccc cggcaccatc ggcaccgtgc actcggtgcc caggctgatg gccggccaag
     6721 gcgcgatcat cggtgtcggc gccatggaat accccgccga gtttcagggc gcgagcgcag
     6781 agcgcatcgc ggagctgggt atcggcaagc tgatcaccct gacctcgacc tatgaccacc
     6841 gcatcatcca gggtgcggaa tccggcgact tcctgcgcac catccacgaa atgctgctct
     6901 ccgacgattt ctgggacgag atcttccgcg agctgggcaa cccgtacctg ccggtccgct
     6961 ggagcaccga caacccggac tcgatcgtcg acaagaacgc ccgggtcatg gagctgatcg
     7021 ccgcctaccg caaccgcggc cacctgatgg ccgacgtcga tccgctgcgc ttggaccaaa
     7081 ccaggttccg cagtcacccc gacctcgagg tgctgaccca cggcctgacg ctgtgggacc
     7141 tcgatcgggt gttcaaggtc aacggcttcg ccggttgcga gtacaagaag ctgcgcgacg
     7201 tgctgggcct gctccgcgac gcctactgcc gccacatcgg cgtcgagtac acccacatcc
     7261 tcgagcccga acaacagaaa tggctggagc agcgggtcga gaccaaacac gtcaaaccca
     7321 ctgtggccga acagaaatac atcctgagca agctgaacgc cgccgaggcc ttcgagacgt
     7381 tcctgcagac caagtacgtc gggcagaagc ggttctcgct ggaaggcgct gaaagccaga
     7441 tcccactgat ggatgcggcg atcgaccagt gcgccgagca tggcctcgac gaggtggtca
     7501 tcgggatgcc gcaccgcggc cggctcaatg tgctggccaa catcgtcggc aagccgtact
     7561 cacagatctt cagcgagttc gagggcaacc tcaacccgtc acaggcgcac ggctccggcg
     7621 acgtcaaata ccacctcggc gccaccggcg tgtacctgca gatgttcggc gacaacgaca
     7681 ttcaggtgtc actgaccgcg aacccgtcgc acctggaagc cgtggacccg gtgctggaag
     7741 ggctggttag ggccaagcag gacctgctgg acccaggcga tgacggggag acaaaagcgt
     7801 tctcggtcgt ccccatgatg ctgcacggtg acgccgcgtt cgccggccag ggagtcgtcg
     7861 ccgagacgct gaacctggcg aacctgcccg gttaccgcgt cggcggcacc atccacatca
     7921 tcgtcaacaa ccagatcggc ttcaccaccg caccggaata ctcccggtcc agcgaatact
     7981 gcaccgacgt cgccaagatg gtcggggcgc cgatcttcca tgtcaacggc gacgacccgg
     8041 aagcctgcgt gtgggtggcg aagctggcgg tggacttccg gcaaaaattc aacaaggacg
     8101 tcatcatcga catgctctgc taccggcggc gggggcacaa cgagggcgac gacccgtcga
     8161 tgaccaaccc gtacatgtac gacgtggtcg acaccaagcg cggggcccgc aagagctaca
     8221 ccgagtccct gatcggccgc ggtgacatct cgctcaagga agccgaggac gcgctgcgcg
     8281 actaccaggg ccagctggag cgggtgttca acgaggtccg cgagctggag aagcacggtg
     8341 cgcagccgag cttgtcagtc gaggaagacc agatgattcc ggcggggctg gacaccgcgg
     8401 tcgacaagtc gctgctggcc cgcatcggtg acgcattcct ggccctaccc gacggtttca
     8461 ccgtgcaccc gcgcgtgctg ccggtgctgg aaaggcgccg ggagatggcc tacgaaggca
     8521 aaatcgactg ggccttcggc gagctgcttg cgttgggttc gctggtggcc gagggcaagt
     8581 gggtgcggtt ctccgggcaa gacaccaagc gcggtacctt ctcccagcgg cattcggtga
     8641 tcatcgaccg cagcaccggc gaagagttca caccgttgca actgttggcg acaaaccccg
     8701 atggcaggtc caccggcggc aagttcctgg tttacgactc accgctgtcg gagtacgcca
     8761 cggtcggctt cgagtacggg tacaccgtgg gcaacccgga cgcacttgtg ctgtgggagg
     8821 ggcagttcgg cgacttcgtc aacggcgcac agtcgatcat cgacgagttc atcagttccg
     8881 gtgaggccaa atggggtcag ttgtccaacg tggtgctgct gctgccgcac gggcatgagg
     8941 ggcagggccc cgaccacacc tccggtcgga tcgaacgctt cctgcagctg tgggcggagg
     9001 gctcgatgac gatcgcggtg ccgtcgactc cggcgaacta cttccatttg ttgcgtcgac
     9061 atgccctcga cggcatcgcg cgcccgctga tcgtgttcac accgaagtca atgctgcgca
     9121 acaaggcggc cgtcagtgac atcaaggatt tcaccgagat caagttccgc tcggtgctgg
     9181 aggaggccac ctacgaggac ggcatcggcg accgcagcaa ggtcaggagg atcctgctga
     9241 ccagcggtaa gctctattac gagctggcgg cacgcaaggc caaggacaag cgcgacgaca
     9301 tcgcgatcgt gcgcatcgag cagctggcgc ccgtgccgaa gcgccggctc agcgaaaccc
     9361 tggaccggta tccgaacgcc ggcgagttct tctgggtcca ggaagagccg gccaaccaag
     9421 gcgcctggcc gcggttcggc ttggagctgc ccgagctact gcccgacaaa ttgaccggaa
     9481 tcaagcgcgt ctcacgccgg gcgatgtccg cgccgtcgtc gggctcgtcg aaggtgcacg
     9541 ccgtcgagca gcaggagatc atcgacaccg cgttcggcta gcccggcgac ggtgagcgcc
     9601 gcaacggcgc gagtgaggag ccgggcaatc ggggcaggcc cggcgacggt gagcgccgca
     9661 acggcgcgag tgaggagccg ggcaatcggg gcaggcggcc gacccacccg ccggtatcgg
     9721 ggaccgctgg taccgggtaa cctcgatgac agccgacact agggagcgtg ttatgcgggg
     9781 gttcgcaggc aaggtagccg cggttaccgg cgcgggttcg ggcatcggac aggcgctggc
     9841 cgttgagctg gcccgctcgg gggccaagct ggcgatcagc gacgtcgaca ccgaaggact
     9901 ggcgcaaacc gaggagcagt tgaaggcgat cggtgcgccc gtcaaagcgg gtcggctcga
     9961 cgtgaccgag cgcgaggcat tcctggccta cgccgacgcg gtcaacgacc acttcggcca
    10021 ggtcaaccag atctacaaca acgccggcat cgcgttcacc ggcgacatcg aagtcagcca
    10081 gttcaaggac atcgaacggg tgatggacgt cgacttctgg ggcgtcgtga atggcaccaa
    10141 ggcgttcctt ccgtatctga tcgcctccgg cgacggccac gtgatcaacg tctccagtgt
    10201 cttcgggctg ttctccgtgc cggggcaggc ggcgtacaac tcggccaagt tcgccgtgcg
    10261 cggcttcacc gaggcgctac gccaggagat ggtgctggcg ggccacccgg tgggggtgac
    10321 cacggttcac cccggcggca tcaagaccgc gatcgcgcgc aacgccacgg cggccgaggg
    10381 actcaaccgc gacgagctgg ccaagctgtt cgacaaacgg ctggccaaga ccagtccaca
    10441 acgcgccgcc gagatcatcc tggaggctgt ggacaagaac aaggcccggg tgctggtcgg
    10501 cacggacgcc aagctcttgg acctgatggt gcggctgagc ggttccgggt atcagcgcat
    10561 tttcgggtcc gtgctgggcc ggttggggct gtcgaagcac tgacggtcag cactctcagc
    10621 ggcccagcgg gtgctcggcc aaccatccgc ccgccactgc ctgcggaccg gcgccgccgg
    10681 ccagttggcg gcgcatctcg gccagcgccg cggtgtccag cacgccggcc acttcgttga
    10741 ccgccaggag ctggcgatcg ctcagcgcgt tgcggcgata gagcggcacc acgttctcgg
    10801 cctgtatgag cgcgggtttg ccgtcgggca gcgcggtcaa gtcggcggga atgccggggt
    10861 cggcggtgct cgtccagcca gcggtcggct gtccgttccg caccgcggcg aacagtgttg
    10921 cttggtcggc gaattcgcgc ggagcgggga gccggcaaga tccgacgacc gagggctcct
    10981 cgaacccggt gaccgccccg atcgtcagcc catcgcaatg gctcggcagc gtgctgaggt
    11041 ccgcgctcgg ctcatggccg gcccaggcgc tggcggtggc ttgcgacacc agcagcaggg
    11101 gcttgtctgc ggcggcggtg gtgtagtcgc ccgccaagat gccttcgggc agggccgcga
    11161 tcatcgcgcg atagacctgc gcgtcggaca gggcgaatgc gccgggctgc aggcgccgca
    11221 aggtctgtcc ggtaaacgcg ggaaggacgg tgaactcgcc cgagtccagc ttcgccatcg
    11281 ggtcttcggc ggtgtcggcc cgggcggcga aaccgtatga ctgcaacgcc gccacgtaga
    11341 tcgcggccag cagcatcgat tcggaatcgg gtcgggatcc gaccaccacc tccgaagggt
    11401 gatcgccggt gtgggtcgtg cagccggccc agagaaccac agcgagcgtg gcggcgagca
    11461 gcgccgcggc aagcttgccg attctcactc ccaggaaact gcagcgtctg agacttctgc
    11521 ggccagcgct accgcactgg ccaccgcttc accgacgcgc aagtccagtg ggctcgggac
    11581 gatccggtcg accgcgaggt cgtcgctgac gacggcgaag atcgcctcgg ccgcggccac
    11641 catcatcttt tcggtgatcc ggcgggcgcc ggcgtccagc gcgccgcgga acaacccggg
    11701 gaacgcgagc acattgttga tctggttcgg gaagtcgctg cgtccggtgg ccaccaccgc
    11761 cgcgtacttg gccgcgatgt gcgggtgtat ctccggatcc gggttggaca acgcaaacac
    11821 gatcccgtcg ggcgccatgg tggcaatcag ctcctcgggg accacgcccg ccgacacccc
    11881 gaggaagacg tcggcgccct tgagcgcttc gctcatgccg ccgctgagac tactcggatt
    11941 ggtgcgtcga gccagatcga ccttgacgct gttcatgtcg ttgcggccgg tgtgcaggat
    12001 gccgcgcgag tcgagcacgg tgatgtgtga aactcccatt gccagaagca gattcgtgca
    12061 tgccaccccc gcggcgccgg caccggacac cactacgcgc accgaggaca tgtcgcggcc
    12121 gagcaacttg gtggcgccca tcaacgcggc cagcacgacg atcgccgtgc cgtgctggtc
    12181 gtcgtgcatc accggacagt ccagagcctc gatgactcgt cgttcgatct cgaaacagcg
    12241 cggagcggag atgtcctcca ggttgacggc gccgaacgtc ggccgcagcc ggaccagagt
    12301 ctcgacgatc tcgtcgggat ccttggtggc caacacgatc gggatggcgt tcagtccacc
    12361 gtatgcctga aaaagggcac acttgccctc catcacgggc agcgacgcct cgggcccgat
    12421 gtcacccagt ccgagcaccg cgctgccgtc ggtgacgacg gccaccagcc ggttcgccca
    12481 ggtgtagcgg gcagcgagcg tgcgatcggc ggcgattgca cgactgacct gcgcgacacc
    12541 cggggtgtac gcaatcgaca gagcccgctg ggtgtcgagc ggggatttca gctcaacgag
    12601 gagcttcccg cctacgtgcg catcgaaaat ttcgtcgttt ccgataacga tctgcggtag
    12661 cacgctttcg ggcacggcac tagggtactg tgccccaacc gccagccggt ccgggcccgg
    12721 gtggccgatg cgtcagatca gaccgaggcc ggtgaccgcg ttgcgctcgt cggccagctc
    12781 cgcggtcgat gcgtcgatcc ggccgcggga gaactcatcg atcttcaggc cgccgacgat
    12841 ctcccagttg ccgtctttgg tggtcaccgg gaacgacgaa atcagccctt cgggcacacc
    12901 gtaggaacca tcggagacga cggccattga gacccagttg tcggcggggc tgccgagcaa
    12961 ccagtcgcgg gcggcgtcga cggtcgccga cgcggccgag gcagccgagg aggcgccgcg
    13021 cgcgtcgatg atcgccgcgc cgcgcttggc gacggtcggg atgaagtcct tctcgatcca
    13081 ggcctggtcg cccacgacct cagccgcgtt cttgccgccg acctcggcg
//
LOCUS       DASSGQ010000061         4240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1492845,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000061 DASSGQ010000000
VERSION     DASSGQ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492845"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VFI55_02555"
     CDS             <1..701
                     /locus_tag="VFI55_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003916985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET7072948.1"
                     /translation="AAMLALAVTGAIILSRLFWGEATTVLLRIVDRREVQRTRRVTWR
                     ARMVTAWAGFRGAVSLAAASAIPMTIHGGQPFPDRSLIIYVVVVVILLTVIIQGTTLP
                     AVVRWARMPDDVAHAEELRLARTRGTQAALDSLPSVASALGVDPDILNRIQQEYEKRA
                     ALILATEDGPERSRLAELKNQVRRVRLGVLEHKRQEITALRNQNLIDDIVLRELQAEM
                     DLEEVQLLNATEND"
     gene            773..1420
                     /locus_tag="VFI55_02560"
     CDS             773..1420
                     /locus_tag="VFI55_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7072949.1"
                     /translation="MTDQPRGLRERKKADTRRAISDAALNLAFERGLDNVTRGDIASL
                     AGVSLRTFNNYFNGKYQALAYRQTERMRRSLAALRQRPADEPLWTSVTHAALEPLDDD
                     LSDARGDEDLVPSRQELLEVRKLLMNPQVRKALPQELFDEWLEVIAERTGTDPDRDMY
                     PRLVVAVMRAVSDAAAEAYVRADPPVAITDLIRAAFAAVSVDQPEPTERREAHHG"
     gene            1413..2939
                     /locus_tag="VFI55_02565"
     CDS             1413..2939
                     /locus_tag="VFI55_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7072950.1"
                     /translation="MADERADIVIAGAGPNGLMLACELALAGLRPVVLDAQSGPSSEP
                     KANGLVGQVVRMLDMRGLYQAFTGDADPPEPSYGWIFAGMTLNFLGMTDNPMYAMLLP
                     QPRLVRLLEKRARDLGVDLRWGHELVGLAQEPESVAVSVSSPEGDYRIAARYLVGADG
                     GRSLVRKTVGIDFPGSTSETVGRLAHVHVPEELRGADGGVDIPGFGRLPFGHTRLDNG
                     GAIFAEFEPGRALFGTIEFEPPVEDKPMSFDELRDSARRVLGGVTVPFEEPKGPGPHA
                     LRRINGQNTRLAERYRDGRVLLLGDAAHVHSPMGGPGLNLGLQDAVNLGWKLAAHLNG
                     WAPAGLLDTYESERHPVGQRVMMQSLTQTALMAPGPEVAAARALLGELFTIPDVAKHM
                     AGLLAGSDVRYDVGDDHPLSGRLVPHLTLEDGRRVAELLHDARPVLLDVDGAFADAAR
                     GWADRVDIIGAAIADRPAAALLIRPDGYVAWAADTFTATGEAGLRTGLQRWFGPASRG
                     "
     gene            2966..>4240
                     /gene="alsS"
                     /locus_tag="VFI55_02570"
     CDS             2966..>4240
                     /gene="alsS"
                     /locus_tag="VFI55_02570"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HET7072951.1"
                     /translation="MAPTTPGQPVRSAQKIVDVLSAQGVEYIFGVPGAKIDPVYDALV
                     DGGPELVVCRHEQNAAFMAGAIGRLTGNPGVVLVTSGPGTTNLATGLLTANTEQDPVV
                     ALCGAVRREDRLKRTHQSMDAAAMLKTVTKFTGEINDPDNAAEATIGAFRAAVSEPRG
                     ATAVVLPADVLAAPTTAEITARLPIPPMAAAPAAAIAEASELIRGASRPALLVGIRGA
                     DPDSCQALRALVAATGLPVVETFQAAGVASRSLEDHFLGRVGLFRNQPGDVILAHADA
                     LIAIGYDSVEYDPVLWNDNVERTVIHIDSLPADIDNSYQPTQELLGNIGATLTALTEN
                     LAGLTLASDYHAEIGLQRKALADIDANARDETPDGPGLNPVAVVLRLREELDDTATIA
                     CDIGSVYIYMARHFRVYEPRRLLFSNGQQTLGV"
BASE COUNT          731 a         1496 c         1367 g          646 t
ORIGIN      
        1 atgccgcaat gctcgctctc gcggtcacgg gcgccatcat cttgtcccgg ctcttctggg
       61 gagaagccac cacggtgttg ctgcgtatcg tcgaccgccg cgaggtgcag cgcacccgcc
      121 gagtcacctg gcgcgcacgc atggtgaccg cgtgggcggg attccgcggc gcggtgtcgc
      181 tcgccgcagc gtcggccatc ccgatgacga tccatggcgg ccagccattc cccgaccgga
      241 gcctgatcat ctacgtcgtg gtcgtcgtca ttctgctgac cgtgataatc caagggacca
      301 cgctgcccgc cgtggtccgc tgggctcgaa tgcccgatga cgttgcccac gctgaagaac
      361 tgcgactggc ccgcactcgc ggtacccagg ccgccctcga ctcgctgcca tcggtggcca
      421 gcgcgctggg cgtcgacccg gacatcttga accgcataca gcaggaatac gaaaaacgcg
      481 ccgcgctcat tctggccacc gaggacggcc cggagcgcag ccgattggcc gagctcaaga
      541 atcaggttcg gcgggtgcgt ctgggtgtgc tcgagcacaa gcgccaggaa atcaccgcgc
      601 ttcgaaacca gaatctcatc gacgacatcg tgctgcgcga gctgcaggcc gagatggacc
      661 tagaggaagt gcaacttctc aacgccactg agaacgacta gctcccccac acagaaattt
      721 cccagcgggc aattttgccc acagtgcaac attttggggt atcttcgtgt ccatgaccga
      781 tcagccgcgc ggcctgcgcg agcgcaagaa ggccgatacc cggcgtgcaa tcagcgatgc
      841 cgcgttgaat ctggcgttcg aacgtggact ggacaacgtg acccgcggag atatcgccag
      901 tctggccggg gtctcgctgc gcaccttcaa caactacttc aacggcaaat atcaggcgct
      961 ggcctaccgg cagaccgaac gcatgcgccg cagcctcgcc gcgcttcgcc aacgtcccgc
     1021 cgacgaaccg ctgtggacgt cggtcacaca cgcggcactc gagcccctgg acgacgatct
     1081 cagcgatgcc cgcggcgacg aagacctggt gcccagccgc caggaactcc tcgaagtccg
     1141 aaaactgctg atgaacccgc aggtgcgtaa ggcgttgcca caggagctct ttgacgagtg
     1201 gctcgaggtg atcgcagaac gtaccggcac cgacccggac cgcgacatgt atccgcggct
     1261 tgtggtggcc gtcatgcgcg ccgtcagcga cgcggctgcc gaggcgtacg tccgggcgga
     1321 tccgccggtg gcgatcaccg acttgatccg ggcggccttc gccgcggtca gcgtggacca
     1381 acccgagccc accgagcgca gggaggcaca ccatggctga cgaacgggcg gacatcgtca
     1441 tcgccggcgc cgggcccaac ggactgatgc tggcctgcga gctggcgctg gccggcctca
     1501 ggcccgtcgt gctggacgcc cagtccggtc ccagctccga accgaaggcc aacggccttg
     1561 tcggacaagt ggttcggatg ctggacatgc gcggcctgta tcaggctttc accggcgatg
     1621 ccgatccgcc cgaaccgtcg tacggatgga tattcgcggg catgaccctg aatttcctgg
     1681 gtatgaccga caatccgatg tacgcgatgc tcctgccgca gccccggctg gtacggctcc
     1741 tggaaaagcg ggcgcgcgat ctcggggtgg acctgcgctg ggggcacgag ctcgtcggct
     1801 tggcgcagga gccggaatcc gttgcggtgt cggtgtcgtc accggagggc gattaccgca
     1861 tcgccgccag gtacttggtg ggcgccgacg gcggacgcag cctggtccgc aagacggtcg
     1921 gcatcgactt ccccggcagc acctcggaga cggtgggccg gctcgcccac gtgcacgtcc
     1981 ccgaggaact ccgcggcgcc gacggtggcg tcgacatccc cggattcggc aggctcccct
     2041 tcggccacac gcggttggat aacggcggag cgattttcgc cgagttcgag ccgggccggg
     2101 cgcttttcgg caccatcgaa ttcgagccgc cggtcgagga caaaccgatg agcttcgacg
     2161 aactccgcga cagcgcacgc cgcgtgctcg gcggcgtgac tgtaccgttc gaggaaccga
     2221 aaggccctgg gccgcacgcc cttcgccgca tcaacggaca gaacacccgc ctcgcggagc
     2281 ggtaccgcga cggccgggtc ctgctactcg gtgacgcagc ccacgtgcac agcccgatgg
     2341 gcggtcccgg gttgaacctg gggctgcagg acgcggtcaa cctcggctgg aagctggcgg
     2401 cccacctcaa cggctgggcg cccgccggat tgctcgacac gtacgaatcc gaacgccatc
     2461 ccgtgggcca gcgcgtgatg atgcagtcgc tgacccagac cgcgctgatg gcgccgggcc
     2521 cggaagtcgc cgcggcgcga gcgcttttgg gcgagctctt caccattccc gacgtcgcca
     2581 agcacatggc cggactgctg gccggctccg acgtgaggta cgacgtgggt gacgaccacc
     2641 cgctttcggg ccggctggtg ccccacctga ccctcgagga tggccggcgg gtcgccgaac
     2701 tgctccacga cgcgcgtccg gtgctgctcg acgtcgacgg cgccttcgcg gatgcggctc
     2761 gcggctgggc cgatcgcgtc gacatcatcg gcgcggccat cgccgatcgg cccgccgcgg
     2821 cgctgctgat ccggccggac ggatacgtcg cgtgggcggc agacaccttc acggcaaccg
     2881 gggaagcggg cctgcggacc gggctgcagc gctggttcgg acccgcatcg cgcggatagc
     2941 agcaccagcg accagactgg gctgcatggc ccccactact cccggtcaac ctgtccgcag
     3001 tgcgcaaaaa atcgtcgacg tcctctcggc acagggcgtc gagtacatct tcggcgtgcc
     3061 cggtgcgaag atcgaccccg tctacgacgc cttggtcgac ggcggcccgg agctggtggt
     3121 gtgccgccac gaacaaaacg cggcgttcat ggccggcgcg atcggtcgcc tcaccggcaa
     3181 ccccggtgtg gtgctggtga cttctggccc cggcaccacc aacctggcca ccggcctgct
     3241 caccgccaac accgaacaag atccggtggt tgcgctctgt ggagcggtac gccgcgagga
     3301 ccggctcaag cgcacccatc agtcgatgga cgccgcggcg atgctcaaga cggtcaccaa
     3361 gttcaccggt gagatcaacg accccgacaa cgccgctgag gccacgatcg gtgcgtttcg
     3421 cgccgcggtc agcgaaccgc gcggcgcgac cgccgtcgtg ctgcccgccg acgttctggc
     3481 cgcccccacg acggcggaaa tcacggcccg actgccgatc ccaccaatgg cggcggcgcc
     3541 ggccgcagcg atcgccgagg caagcgagct gatccgcggc gcgagccggc cggcattgct
     3601 ggtcggtatc cgcggcgccg accccgacag ctgccaggcg ttgcgtgccc tggtcgccgc
     3661 gaccgggctg cccgtcgtag aaacctttca agccgccggg gtcgcgtccc gcagtctgga
     3721 agatcacttc ctgggccggg tgggactgtt ccgcaaccag cccggcgatg tcatcctcgc
     3781 ccacgccgac gccctgatcg ccatcggata cgactccgtc gaatacgacc cggtgctgtg
     3841 gaacgacaac gtcgagcgca ccgtcatcca catcgactcg cttcctgccg acatcgacaa
     3901 cagctatcag cccacccagg aactactcgg caacatcggg gccaccctca ccgccctgac
     3961 cgaaaacctg gccggcctca ctctcgccag cgactatcac gccgagatcg gcctgcagcg
     4021 aaaagccttg gccgacatcg atgccaacgc acgcgatgag acaccggacg ggccggggct
     4081 caaccccgtt gccgtggtgc tgcggctacg cgaggaactc gacgacaccg cgaccatcgc
     4141 ctgcgacatc ggttccgtgt acatctacat ggcccgccac ttccgggtct acgaacccag
     4201 acggctgctg ttttccaacg gacaacaaac cctgggtgtg
//
LOCUS       DASSGQ010000062        10425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_459962,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000062 DASSGQ010000000
VERSION     DASSGQ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10425
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459962"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VFI55_02575"
     CDS             <1..326
                     /locus_tag="VFI55_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007172077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072952.1"
                     /translation="NGHILFAAAAAEALNLPDSNHDWAHASGSPLELSFPASAYEQLR
                     WTQDSLMPWVWRRMRGLSSADGRVPKRPMLEPLSTPSEPRQGRPAESPDIAARFDRRS
                     GILDP"
     gene            323..988
                     /locus_tag="VFI55_02580"
     CDS             323..988
                     /locus_tag="VFI55_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007172078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET7072953.1"
                     /translation="MNVDALLQSIPPLAVYLVVGGVVGIESLGIPLPGEIVLVSAALM
                     SSHHDLAVNPVGVGTAAVIGAVVGDSIGYSIGRRFGMPLFDRLGARFPKHFGPGHVAL
                     AEKLFNRWGVRAVFFGRFIALLRIFAGPLAGALKMPYPRFLAANVSGAICWAGGTTAL
                     VYYAGMAAERWMERFSWIALVIAVVGGAIAAFVLRERTSRAIAELEAEHSGKTGTTAA
                     DAA"
     gene            complement(1007..1627)
                     /locus_tag="VFI55_02585"
     CDS             complement(1007..1627)
                     /locus_tag="VFI55_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7072954.1"
                     /translation="MTSHEVTTPAVSARERILAAAYELFSRRGIRAVGTDEVIERAVV
                     ARATLYRHFATKDDLVLAVLERREQLWTHGLIEEQSRQRGETPEEQLLAIFDVMHDWF
                     QLRDGYEGCSFINVLLELGPEHPAGRASIAHIDHVRDIVRHRAFAAGLTDVEDFASSW
                     HILMKGAIILAAVGDLDAGLRARKMASTLIEEHRPVAPAEVGEAVG"
     gene            1793..2230
                     /locus_tag="VFI55_02590"
     CDS             1793..2230
                     /locus_tag="VFI55_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HET7072955.1"
                     /translation="MSLVIAEPMSSPHLMLSTRLVYELADPHSTLRTTTDRSGPAVLI
                     NAGGEIDGCNEHTWRRLVSEAASGVTAPGPFVVDVTGLDFMGCCAFAVLADAARRCRE
                     RGIELRLVSREAIVARIVDACGLNGLLPIYPTADAALAAAARW"
     gene            2268..3386
                     /locus_tag="VFI55_02595"
     CDS             2268..3386
                     /locus_tag="VFI55_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HET7072956.1"
                     /translation="MSGFSSFGPTFGVEEEFLLVDPRTGEPAPRNTAVADEAQRRGVT
                     LQLELSSCQVETTSSVATRSAQLGEELTRLRRTATQAAEACGVRLLASGLPPATPHKF
                     PVTDTPRYRRIGAEFGMIAHEQGICGCHVHVQVPDRTAAIHVSNWLRPWLPSLLALSA
                     NSPVYRNADTGHASWRSVLWRRWPAAGSPPFFASPDEYDRVVRMLVDTGVILDEDMIY
                     WDVRPSAQFPTVEVRVADVPATVAETVLLATLIRAAVMTALDARKEGHEKGDAPARLP
                     PGALRAAYWKAAHDGLSGYALDLINGCGAVPAREQLGVLVHRVRAALEALGEYDRVIG
                     ELDRIAAQGNGAMRQLRAWRKRGNVLDVVEEAAAATLR"
     gene            complement(3407..4192)
                     /locus_tag="VFI55_02600"
     CDS             complement(3407..4192)
                     /locus_tag="VFI55_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7072957.1"
                     /translation="MLPSHMVLIALAGLGAGAINALVGSGTLITFPTLVALGYPPVTA
                     TMSNAVGLVAGSVTGTWGYRAELAGQWDRLRWQLPASLAGAALGAFLLLHLPEKVFVQ
                     IVPVLLVLALVLVVAGPRIQSWARRRAEAQGRSPEHITPTRKGVLVLGTFAVGVYGGY
                     FTAAQGILLVGLMGALLPESVQRMNAAKNLMTLVVNVVAAIGYTLVAFDRISWPVAGL
                     IAAGSLVGGWLGARYGRRLSPNALRATIVVVGLIGLYRLVMVA"
     gene            complement(4256..5197)
                     /locus_tag="VFI55_02605"
     CDS             complement(4256..5197)
                     /locus_tag="VFI55_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HET7072958.1"
                     /translation="MTGSVTPDEAIDAIRGTGGAQPGCRALHAKGTLYRGTFTANPVA
                     GGLSRAAHLDGSTVPALVRFSNGSGSPSQSDALPGVRGMAVKFTLPDKSTTDVSTQTA
                     RLFVSSTPDGFVDLLKAMRPGLLTPLRMATYFLTHPRLLGALPVLRDANKVPASYATI
                     EYHGLHAFRWIGVDGSTRFVRYHLVPAAGEKYLSPSAAHGKGADFLTDELNTRLATGP
                     VRFDYRVQIAGPKDSTVDPSAAWRSTQFVTVGTLEIAGLETEREHGGDIVVFDPMRVT
                     DGIEPSDDPVLRFRTLAYSASVKLRTGVDRGAEAPHV"
     gene            complement(5194..5460)
                     /locus_tag="VFI55_02610"
     CDS             complement(5194..5460)
                     /locus_tag="VFI55_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072959.1"
                     /translation="MSVLTIPVSVLPIWFPQRGFQDLARAGVLRDESAEVNSFHSVIR
                     GHAPAGDGGPSSCANIPTRRSPPAGITASSNSSAFPGNAFKIRA"
     gene            5471..6259
                     /locus_tag="VFI55_02615"
     CDS             5471..6259
                     /locus_tag="VFI55_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7072960.1"
                     /translation="MDAQRRDAAISVLGGPTTVVDIAGRRIVMDPTFDPPGVHAYLTK
                     LTGPAVSADALGPVDAVLISHDQHPDNLDDDGRRMALGAPLVLTHPGAAGRLGPPAIG
                     LPPWQSYNLPGGRGALAVQAVPAVHGPADGQRDAGGHINCEVTGFVLSGPGLPTVYLS
                     GDNASMGAVKDVADRVGDIDIAVLFAGAASVPTKEHGRPLTLTSARAVAAAEVLGAKV
                     VIPAHVDGWAHFSGGIDEFVAAFDAAGIGSVLHVAAHGEWIDCR"
     gene            complement(6327..6620)
                     /locus_tag="VFI55_02620"
     CDS             complement(6327..6620)
                     /locus_tag="VFI55_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007172090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072961.1"
                     /translation="MAGNQEGIGVLKLQCPQAHPVGRILREAPHHPVMYDPGAVVGPR
                     RFWPDEDEQPRFTAHCRVCDKPVGEVTATLQSQLSTLIADMTQSVGTAMLSYL"
     gene            complement(6677..7204)
                     /locus_tag="VFI55_02625"
     CDS             complement(6677..7204)
                     /locus_tag="VFI55_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072962.1"
                     /translation="MKAQTAAALAVLAAAACTRQASGPPPSSTATAPANTQTAATPTT
                     SGLKPGPHSPLADVDLPAGATFAGSSSLEERWNYGTSYAATAAFLSKQFARGRKYDAR
                     GATWWRALPPCYNGTHHESPPEGWVTDDSTRWVWADANTSLSVQIFRPSSTVTPSEIV
                     IDYMRPDDSYVCNRQ"
     gene            complement(7324..8979)
                     /locus_tag="VFI55_02630"
     CDS             complement(7324..8979)
                     /locus_tag="VFI55_02630"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HET7072963.1"
                     /translation="MPDEKRGQDALFDALTTKGTAFSVAERRRYGLLGLLPTVVKTLE
                     EQAEHCWHEFSTRHDDLDKHIYLRALQDRNETLFYRVLHDHIAESLPIVYTPTVGEAC
                     QRFSEIYRRPRGLFVSYADRDCLDEVLGNRPQRDVDVIVVTDGQRILGLGDQGIGGMG
                     IPIGKLSLYTLVGGIDPARTLPIVLDVGTDNVELLDDPQYLGWRHRRIDDDEYDEFID
                     RFVATVHKHLPDVLLQWEDFATVHARPILARYRDKLLTFNDDIQGTAAVTLGALHGAA
                     KVAGHPLSQLQVVMLGAGSAGIGVLDMIRREMETEGLSEQAARERIWVVDVVGLLTDD
                     RTDLSAEQREYAQPANRVAGWGLSGPAQLDDVVHNVDVGVLLGLSTAAGAFTESIVRE
                     LAGKTKRPIIFPLSNPTSRAEAHPAELDQWTDGRALIATGSPFAPLRRNGVEYPVAQC
                     NNVYIFPAMGLAVTAAQATRVTDEMMRVAAATLGDASPALADPDQPLLPAWSELPDVA
                     VRIAHAVAVQAVADGVAPKRSDQEIAERIAQVHWIPDYPPPPA"
     gene            complement(8982..10370)
                     /locus_tag="VFI55_02635"
     CDS             complement(8982..10370)
                     /locus_tag="VFI55_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinic semialdehyde
                     dehydrogenase"
                     /protein_id="HET7072964.1"
                     /translation="MAIATVSPVTGEVLKSFDELTPEELEAKLARAAAAAASYRLTSV
                     EQRAGWLGRAADHLEKEADSVSRLITTEMGKTYRAAHEEVAKCVRGLRFYAAEGPAFL
                     RALPGDGARVGAKQTYVTYQPIGVVLAVMPWNLPLWQAMRFAAPALTAGNVGILKHAS
                     NVPQTALYMEQLFRDAGFPDDVFQTLLISARRVEQVLRDDRVEAATLTGSEPAGKSVG
                     AIAGDEIKKVVLELGGSDPFIVMPSTNLERATDVAVRARTLNNGQSCINGKRFFVHED
                     IFDGFLERFVAKMGALVVGDPMDDATDIGPLATESGRDDVEAYVQDAVAKGAKVLVGG
                     KRPDGPGWFYPPTVLTDVTPDMRMYHEEVFGPVAQVWPVASVAEALRLANGHPYGLGS
                     NLWSEDEAERELFIRDVQSGMAFINGNTTSYPEIPFGGVKRSGYGRELSDLGMREFMN
                     AKTVWIGEDAGA"
BASE COUNT         1653 a         3546 c         3508 g         1718 t
ORIGIN      
        1 ccaacgggca catcttgttc gccgccgcgg cggccgaggc tctcaatttg cccgacagca
       61 atcatgattg ggcccacgcg agcggctctc ccctggaact gtcattcccg gccagcgcct
      121 acgaacagct gcgctggacc caagacagtc tcatgccctg ggtgtggcgc cggatgcgcg
      181 gcctgtcttc ggccgacggg cgagtgccca aacgtccgat gctggaaccc ctcagcacgc
      241 cgagcgaacc gcgccagggc aggccggcgg aaagcccgga cattgcggcg cggtttgacc
      301 gccggtcagg catccttgac ccatgaatgt ggatgctttg ctgcaatcca ttccgccgct
      361 cgcggtctat cttgtggtcg gcggtgtggt cggaatcgag agcctgggca tcccccttcc
      421 cggagagatc gtgctcgtca gcgccgcgct gatgtcgtcg catcacgacc tcgccgtcaa
      481 cccggtcggc gtcggcaccg ccgcggtgat cggcgcggtg gtcggcgact cgatcggcta
      541 ctcgatcggc cgccgcttcg gcatgccgct cttcgaccga ctcggcgcgc ggttccccaa
      601 acacttcggt cccggacatg tcgccctcgc cgagaagttg ttcaaccgct ggggcgttcg
      661 ggcggtcttc ttcggccgct tcatcgcgtt gctgcggata tttgccgggc cactggccgg
      721 agccctgaaa atgccgtacc cacgtttcct ggcggccaat gtttcgggcg cgatctgctg
      781 ggcgggcggc actaccgcgc tggtttatta cgccggaatg gccgccgaac gctggatgga
      841 gcggttctcc tggatcgccc tggtcatcgc ggtcgtcggc ggcgctatcg ccgcgttcgt
      901 gttgcgcgaa cgcacatccc gggcgatcgc cgaactcgaa gccgagcact ccggcaaaac
      961 cggcacgacc gcggccgacg ccgcgtgaaa tgcgtgcctc ggcgggctaa cccactgcct
     1021 cgccgacctc ggccggcgcc accggccggt gctcttcgat cagcgtgctg gccatcttgc
     1081 gagcgcgcag cccggcgtcc aggtcaccca ccgccgccag aatgatggcg cccttcatca
     1141 ggatgtgcca tgacgaggcg aagtcctcga cgtcggtcag ccctgccgca aaagcgcggt
     1201 gacgcacgat gtcgcggacg tggtcgatgt gcgcgatgct ggcccgtccg gccggatgct
     1261 ccggccccaa ctcgagcaac acgttgatga acgagcagcc ctcgtaaccg tcgcggagct
     1321 gaaaccagtc gtgcatgacg tcgaagatcg ccagcagctg ttcttcgggc gtctcgcccc
     1381 gctggcgtga ctgttcctcg atcaacccgt gcgtccacag ttgttcgcgc cgctccagca
     1441 cggccagtac gaggtcgtcc ttggtcgcga aatgacggta gagggttgcc ctggcgacaa
     1501 cggcccgctc gatcacctcg tcggtgccga cagcccggat cccgcggcgg ctgaacagct
     1561 cgtacgctgc cgccaggatg cgttcacggg ccgacacggc cggcgtcgtg acctcgtggc
     1621 ttgtcatagc gagccttacc atactcttag cggcgacagg tagacagacc gccctgtctc
     1681 gttatacaaa tgagattcgc ggttcgccaa tcgcgagtcg tctgtcttca gacagcagga
     1741 cgggcatcca tcctggcgcc accggcgccg gtcgattagg gagtccacga ttatgagcct
     1801 ggttattgca gaacccatgt ccagcccgca tttgatgctg agcacgcggc tggtctacga
     1861 gctggccgac ccgcacagca cgctgcggac gaccacggat cgcagcggcc cggcggtgct
     1921 catcaatgcg ggcggcgaga tcgacggctg caacgagcac acctggcggc ggctggtcag
     1981 cgaggcggca agcggcgtga ccgcgccggg acccttcgtg gtcgacgtca cgggactcga
     2041 tttcatgggt tgctgtgcgt tcgcggtgct ggccgacgcg gcgcggcggt gccgggagcg
     2101 cggcatcgaa ttgcgtttgg tgagccgtga ggcgatcgtc gcccgcatcg tcgacgcgtg
     2161 cgggctcaac ggcctgctac ccatctatcc gacggcggac gccgcgctcg cggcggcagc
     2221 ccgctggtaa agccgaccgg cgcccctgat ctgccgtacc gggccggatg agcggcttct
     2281 ccagtttcgg gcccaccttt ggtgtggagg aggaattcct tctggtcgac ccgcggacgg
     2341 gcgagccggc gccccgcaac accgcggtgg ccgacgaggc gcagcgccgg ggcgttacct
     2401 tgcagctcga gttgagcagt tgccaagtcg aaaccacctc cagcgtggcg acgaggagcg
     2461 cccagctcgg tgaggaactc acccggctgc gacgcaccgc tacgcaggcc gccgaggcct
     2521 gtggggttcg gctgctggct tcggggctgc cacccgcgac gccgcacaag tttcccgtca
     2581 ccgacactcc ccgttaccgc cggatcggtg cagagttcgg gatgatcgcg cacgagcaag
     2641 gcatctgcgg atgtcacgtg cacgtgcagg tgcccgaccg gaccgccgcc attcacgtca
     2701 gcaactggct gcggccctgg ctgccgtcac tgcttgcgtt gtcggccaat tcgccggtat
     2761 atcgcaacgc cgacaccggc cacgcgagct ggcgcagcgt gctgtggcga cgttggccgg
     2821 ccgccgggtc gccgccgttc ttcgcctcac ccgacgaata cgaccgcgtg gtccgcatgc
     2881 tcgtcgacac cggggtgatt ctggacgagg acatgatcta ttgggacgtg cggccgtcgg
     2941 cgcagttccc cacagtggag gtccgggtcg ccgacgtgcc ggcgaccgtc gccgaaaccg
     3001 tgctgcttgc caccttgatc agggccgcgg tgatgacggc gctcgacgcg cgcaaagagg
     3061 gccacgaaaa gggtgatgcg cctgcgcgcc tgccgcccgg cgcgctgcgg gcggcctact
     3121 ggaaggccgc ccacgatgga ctgtcggggt acgcgctcga cctgatcaac ggctgcggag
     3181 cggtgccggc gcgtgagcag ctgggcgtgc tggtgcaccg ggtgcgcgcg gcgttggagg
     3241 cgctcggtga atacgaccgg gtgatcgggg aactcgaccg gatcgccgcg cagggcaatg
     3301 gggcgatgcg gcagctacgg gcgtggcgca aacgcgggaa cgtgctggac gtcgtcgagg
     3361 aagccgccgc ggccacgttg cgttgagtag cgccgccggc ccttcgtcac gccaccatga
     3421 ccagccggta gagcccgatc aggccgacca ccacgatggt ggcgcgcaac gcattcggcg
     3481 acagccggcg cccgtagcgc gccccgagcc atcccccgac cagcgaaccc gccgcgatga
     3541 gaccggcgac cggccagctg atccgatcga aggcgaccag cgtgtagcca atcgccgcaa
     3601 ccacattgac taccagggtc atcaggttct tggccgcgtt catgcgctgc acggactccg
     3661 gcagcaacgc acccatgagg ccaaccagca agatgccctg agcggcggtg aagtagccgc
     3721 cgtagacgcc gacggcgaac gtgcccaaca ccagcacacc ctttcgtgtc ggtgtgatgt
     3781 gttccggtga gcgtccctgc gcctcggccc gtcggcgcgc ccacgactga atccgcggcc
     3841 cggccaccac cagcaccagg gccagcacca acagcaccgg cacgatctgg acgaacacct
     3901 tttcggggag gtgcaggagc aggaaggccc ccagcgcggc gccggccaac gatgccggca
     3961 gctgccaccg cagccgatcc cactgacccg ccagctcggc gcgataaccc cacgtgcccg
     4021 tcacgctgcc ggcgaccaga ccgaccgcgt tcgacatggt ggcggtgacc ggcggatagc
     4081 cgagcgcgac gagcgtgggg aacgtgatca gggtgcccga cccgaccagc gcgttgatgg
     4141 cgccggcgcc gagcccggcc aaggcgatca aaaccatatg ggaaggcagc acttgtgcac
     4201 acgttagtcc gccgccgcgc cgccccaaaa accgcgtggc cgagcgcctt cgacgtcaga
     4261 cgtgcggcgc ttccgcacct cgatccacgc cggtgcgcag cttgaccgat gcggaatagg
     4321 caagggtacg gaaccgaagg accgggtcat cggagggttc gatgccgtcg gtcacgcgca
     4381 tcgggtcgaa cacgacgatg tcgccgccat gttcgcgctc ggtctcgagt ccggcgatct
     4441 cgagcgtgcc gacggtgacg aactgtgtgc ttcgccacgc cgccgacggg tcgaccgtcg
     4501 agtccttggg accggcgatc tgaacgcggt agtcgaatcg cacggggccc gtcgcgaggc
     4561 gggtattcag ctcgtcggtg aggaagtcag cgcccttgcc gtgggccgcg gacggcgaca
     4621 ggtatttctc acccgcggcc gggaccagat ggtaacgaac gaatctggtg ctgccgtcga
     4681 caccgatcca ccggaatgcg tgcaagccgt ggtattcgat ggtggcgtaa ctggccggca
     4741 ccttgttcgc gtcgcgcaac accggcaacg cgccgagcag ccggggatgg gttaggaaat
     4801 atgtggccat gcgcagcggc gtcagcaggc cgggccgcat cgccttcagg aggtcgacga
     4861 atccgtccgg cgtgctggac acgaacaggc gggcggtctg cgtcgacacg tcggtcgtcg
     4921 acttgtcggg cagtgtgaac ttcaccgcca tcccccgcac ccccggcagc gcgtcgcttt
     4981 gtgacgggct tcccgatccg ttggagaacc ggaccagcgc cggcacggtc gaaccgtcga
     5041 ggtgcgccgc gcgcgacagc ccgcccgcca cggggttcgc ggtgaacgtt ccccgataca
     5101 gcgtgccttt ggcgtgcaat gcccggcagc cgggctgcgc accgccggtg ccccgaatcg
     5161 cgtcgatcgc ctcgtcaggt gtgacagaac cggtcatgcg cgaatcttaa aggcattacc
     5221 ggggaaagcc gaagagtttg acgacgccgt gatcccggcc ggcggcgaac gacgcgtcgg
     5281 aatgttggcg cacgacgagg ggccgccgtc gccggcggga gcgtggccgc gtatcacaga
     5341 gtggaacgaa ttcacctcgg cggattcgtc gcgcaggacg cccgcgcggg caagatcctg
     5401 aaaaccacgc tgcgggaacc atattggcaa aaccgaaacc ggaatggtga gtaccgacat
     5461 gagtgccctc atcgacgcgc aacgacggga cgctgcgatc agcgtgctgg gcggccccac
     5521 gacggtcgtc gacatcgccg gccgccggat cgtgatggac ccgaccttcg atccgcccgg
     5581 ggtgcacgcc tacctgacca agctcaccgg tcccgcggtg agcgccgacg cgctggggcc
     5641 ggtggacgcg gtgttgatca gccacgacca gcatcccgac aatctggacg acgacggccg
     5701 tcggatggcg ctgggcgcgc ccctggtgct gacccatccc ggtgccgcgg gccggttggg
     5761 accgccggcg atcggattgc cgccctggca gtcgtataac ctgcccggcg gccgcggagc
     5821 ccttgcggtg caagctgttc cggccgtgca cggcccggcc gacgggcaac gcgacgccgg
     5881 cggtcacatc aactgcgagg tgaccggctt cgtgctatcc ggacccggcc taccgaccgt
     5941 ttacctcagc ggcgacaacg cctcgatggg tgccgtcaag gacgtcgccg atcgcgtcgg
     6001 cgacatcgac atcgcggtgc tcttcgcggg ggccgccagc gtgccgacca aggagcacgg
     6061 tcggccgttg acgctgacgt cggcgcgcgc cgtcgcggcc gccgaagttc tgggcgccaa
     6121 ggtggtgata ccggcccacg tcgacggctg ggcgcacttc agcgggggca tcgacgaatt
     6181 cgtcgcggcg ttcgacgcgg ccggaatcgg cagcgtgctg cacgttgccg cgcacggcga
     6241 atggatcgac tgccgctgac cggccgccac cacaggcgtc cctgcacccg cagctccgta
     6301 tgcacctaat cgggcgcgcg ccgcccctac agatacgaca gcatcgcggt cccgacagac
     6361 tgggtcatgt cggcgatcaa ggtgctcagt tggctttgga gcgtcgccgt gacttccccc
     6421 accggtttgt cacagacccg gcagtgcgct gtgaatcgcg gctgctcgtc ttcgtccggc
     6481 cagaatcgtc gtggcccgac gacggccccg gggtcgtaca tgaccggatg atgtggggcc
     6541 tccctgagga tcctgcccac cggatgggcc tgcgggcatt gaagtttcag cacgccgatc
     6601 ccctcttggt tgcctgccat cgtccaccgt cctcacgggt ctgctctgca cgcgattgtc
     6661 agccgcgtgc gctgctctac tgcctattgc acacgtacga gtcgtccggg cgcatgtagt
     6721 cgatcacgat ctcgctggga gttaccgtgc tgctcggccg aaagatctgg acggagagcg
     6781 aggtgttggc gtccgcccac acccaacgcg tggaatcgtc ggtgacccaa ccctccgggg
     6841 ggctctcgtg gtgtgtgccg ttgtagcagg gcggcagggc gcgccaccac gtcgcaccgc
     6901 gcgcgtcgta cttgcgtccg cgggcgaact gcttgctcag gaacgccgcg gtggccgcat
     6961 aggaggttcc gtagttccag cgttcttcca gcgagctgct gcccgcgaag gtcgcgcccg
     7021 cggggagatc gacgtcggca agcggggaat gcggtcctgg cttcagcccg gacgtcgtcg
     7081 gcgttgccgc cgtttgggtg ttcgccggcg cggtcgcggt cgacgacggc ggcggcccgg
     7141 aggcttgccg cgtgcatgcg gcggcggcga gcaccgccaa ggcggccgcg gtctgtgcct
     7201 tcatcgttct ccgagttggc tatcgcgcat ggcggggagc cggctatcga gacccaccgt
     7261 accgctggac ggctgcggcg cgtgcgggtc gcacagaggc gatgcgtgtc gacagctggc
     7321 ccgtcaggct ggcggtggag ggtagtcggg aatccagtgc acctgtgcga tccgttcggc
     7381 gatttcctgg tcgctgcgtt tcggggcgac gccgtccgcc acggcctgca cggcgacagc
     7441 gtgggcaatg cggacggcca cgtcgggcag ctctgaccac gcgggcaaca acggctggtc
     7501 ggggtcggcg agggcggggg acgcatcgcc cagggtggcc gcggcgaccc gcatcatttc
     7561 gtcggtgact cgggtcgcct gcgccgcggt caccgcaagc cccatcgcgg ggaagatgta
     7621 gacgttattg cattgggcca caggatattc cacgccattg cggcgcagcg gagcgaacgg
     7681 tgagcccgtg gcgatcagcg cccgcccgtc ggtccactgg tccaattcgg ccgggtgcgc
     7741 ctccgcgcgg ctcgtcgggt tggagagcgg aaagatgatc ggccgcttgg ttttgcccgc
     7801 cagttcgcgc acgatgctct cggtgaacgc accggccgcg gtggacagac ccaacaaaac
     7861 accgacgtcg acgttgtgga cgacgtcgtc gagctgggcc ggacccgaca atccccagcc
     7921 ggccacccgg ttggcgggct gagcgtactc gcgctgttcc gccgacaggt cggtgcggtc
     7981 gtcggtcagc agccccacca catcgaccac ccagatccgc tcccgtgcgg cctgttcgga
     8041 cagtccctcc gtctccatct cccgacggat catgtccagc accccgatgc cggccgagcc
     8101 cgctccgagc atgacgacct gcagctgcga cagcgggtga ccggcgacct tggcggcgcc
     8161 gtgcagtgcg ccgagtgtga cggcggcggt gccttgaatg tcgtcgttga aggtgagtag
     8221 cttgtcgcga tagcgggcca ggatcggccg tgcatgcacg gtcgcgaagt cctcccactg
     8281 cagcagcaca tcgggcaggt gcttgtgcac cgtggcgacg aatctgtcga tgaactcgtc
     8341 gtactcgtcg tcgtcgatgc gccggtggcg ccagcccagg tattgcgggt cgtcgaggag
     8401 ttcgacgttg tcggtaccca cgtcgagcac aatcggaagc gtgcgagcgg gatcgatgcc
     8461 gccgacgagc gtgtacagcg acagcttgcc gatcgggatg cccatcccgc cgatgccctg
     8521 gtcacccagc ccgaggatgc gctgcccgtc ggtcaccacg atcacgtcga cgtcgcgctg
     8581 cggccggtta cccaacacct cgtcaagaca gtcgcggtct gcgtaggaca cgaaaagtcc
     8641 acgcggccgc cggtagatct cgctgaaacg ctggcaggcc tcacccacgg tgggcgtgta
     8701 gacgatcggc agggattcgg cgatgtggtc gtgcagcacc cggtagaaca gggtttcgtt
     8761 gcggtcctgc aaggcgcgca gatagatgtg tttgtcgaga tcgtcgtggc gagtggagaa
     8821 ttcatgccag cagtgctcgg cttgctcttc cagcgtcttc actacggtcg gcagcaggcc
     8881 cagcaaccca tacctgcggc gctccgccac cgaaaaggcc gtgcccttgg ttgtcaaggc
     8941 gtcgaacagg gcgtcctgcc cgcgcttttc gtcaggcatc tctaggcacc tgcatcctcg
     9001 ccgatccaga cggtttttgc gttcatgaac tcacgcatgc ccaggtcgga cagttcccgc
     9061 ccgtacccgc tgcgcttgac cccgccgaaa gggatctccg gatagctcgt cgtgttgccg
     9121 ttgatgaacg ccatccccga ctggacgtcg cggatgaaca gttctcgctc cgcttcgtcc
     9181 tcgctccaca ggttcgatcc cagtccgtac ggatggccgt tggccaaccg cagggcctcc
     9241 gccaccgagg ccaccggcca cacctgcgcc accggaccga acacctcctc gtggtacatc
     9301 ctcatgtccg gggtgacatc ggtcagcacc gtcggagggt agaaccaacc gggcccgtcc
     9361 ggccgtttcc cgcccaccaa caccttggcg cccttggcta ccgcgtcctg gacgtaggct
     9421 tcgacgtcgt cgcgcccgga ttcggtggcc agcggaccga tgtcggtcgc gtcgtccatc
     9481 gggtccccca ccaccagcgc ccccattttg gcgacgaacc tctccaggaa gccgtcgaag
     9541 atgtcctcgt gcacgaagaa ccgcttgccg ttgatgcacg actgcccgtt gttgagggtg
     9601 cgggccctga ccgcgacgtc ggtggcccgc tccagattgg tggacggcat gacgatgaac
     9661 gggtcggacc cgccgagttc gaggaccacc ttcttgatct cgtcgccggc gatggccccc
     9721 accgacttcc ccgcgggctc gctgccggtc agcgtcgcgg cctcgacccg gtcgtctcgc
     9781 agcacctgct ccaccctgcg ggcggagatc agcagggtct ggaagacatc gtccgggaaa
     9841 ccggcgtcgc ggaagagttg ctccatgtac agggcggtct ggggcacatt cgacgcatgc
     9901 ttgaggatgc cgacgttgcc ggccgtcagg gcgggcgcag cgaatcgcat ggcctgccac
     9961 aacggcagat tccacggcat caccgcgagc accacgccga tcggctgata ggtgacgtac
    10021 gtctgcttgg cgccgacgcg cgcgccgtca ccgggcaggg cacgcaggaa cgcggggccc
    10081 tcggcggcgt agaaacgcag cccccgtaca catttcgcca cctcttcgtg tgcggcccgg
    10141 taggtcttgc ccatctccgt cgtgatcaac ctgctgacgg aatcggcctc cttttccaaa
    10201 tggtcggccg cccggccgag ccaccccgcg cgctgttcga cgctcgtcag ccggtatgac
    10261 gccgcggcgg cggccgcccg agcgagtttg gcttcgagtt cctcgggcgt gagttcatcg
    10321 aagctcttca ggacctcacc tgtgacagga cttaccgtcg cgatagccat aggtggatcg
    10381 tatggcctcc aggtgagcca cggactgcgc gcagttgaat tgcgg
//
LOCUS       DASSGQ010000063        21028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_733541,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000063 DASSGQ010000000
VERSION     DASSGQ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 21028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21028
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733541"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..118)
                     /locus_tag="VFI55_02640"
     CDS             complement(<1..118)
                     /locus_tag="VFI55_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HET7072965.1"
                     /translation="MTTPMDRVTHLDLVARLKSTYPDLPDAPTPDLIDHERFT"
     gene            491..883
                     /locus_tag="VFI55_02645"
     CDS             491..883
                     /locus_tag="VFI55_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET7072966.1"
                     /translation="MFLSEIISRRLVVGAIGAGAVTGTLLGAAAGIAAADPPPRPPNC
                     TAADVASVAAGVAASLSTYLFTHPDVNGFYTGLQDQPKDQLRDDVQNYFNANPQEQAD
                     LEGIRQPLVDIRQRCQWTPLLGQDGTTP"
     gene            1042..1335
                     /locus_tag="VFI55_02650"
     CDS             1042..1335
                     /locus_tag="VFI55_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072967.1"
                     /translation="MNTWVRSWSAAGIAVLAITSIPELYAVIQPPGVANADVCASVGR
                     RVSVSGCTNVADTVNTYAPPPAYYAPLPQDYPPPPPVSVCVGAGRRIHVSGCS"
     gene            complement(1418..1603)
                     /locus_tag="VFI55_02655"
     CDS             complement(1418..1603)
                     /locus_tag="VFI55_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072968.1"
                     /translation="MLLVIMGGRLSPNSQLPQEPQVLTFGYNKRRRHEINRGPKDLPF
                     HDFAPTLRASRADLSSP"
     gene            1621..1836
                     /locus_tag="VFI55_02660"
     CDS             1621..1836
                     /locus_tag="VFI55_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7072969.1"
                     /translation="MAAVAVLVAAVAVSVAAVAVSVAAVAASVAAVAASVAAVAASSV
                     AAAVSPKQPNTISSRAAPAVSMRKGHA"
     gene            complement(1981..2523)
                     /locus_tag="VFI55_02665"
     CDS             complement(1981..2523)
                     /locus_tag="VFI55_02665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007471136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7072970.1"
                     /translation="MQTSTSLVRPASRQDADACVAIYRPYVLGTVISWELEVPSVDEM
                     AARIAAAREAHEWLVLERDEEIIGFAYGHAFNRQPAFKWSTETGIYVSVDHHRAGCGR
                     QLYTRLLDRLRERGYRRAFAGITQPNEASTSFHRSFGFERIGLYRRVEWKHDSWHDVA
                     WMQLELRGTADQNQPPGPII"
     gene            complement(2598..3458)
                     /locus_tag="VFI55_02670"
     CDS             complement(2598..3458)
                     /locus_tag="VFI55_02670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET7072971.1"
                     /translation="MKFCVSSAFLNTREIIEIAKAADELGYDGIGIPDHVVNLENLDT
                     PYPYTKDGQRRWQPFTDWPDPWVLVGALAQVTDRLRFVTTVYIPAMRNPYSAAKAIGT
                     AAVLAAGRVELGIGVGWCREEFALMGEQFDARGKRTDEIIELMRALWEPGWTEFDGEF
                     YPTPRLEMQPTPPPIPVYVGGLSDAALRRAARNDGWIGDLITTDRAIEAVGRLRELRI
                     ENGLSLTDFTVLTPLTDAFTGADYQRAAAAGITGIITMPWMFYAGPDATLADKIDGMQ
                     RFRKDLGLDG"
     gene            complement(3455..4081)
                     /locus_tag="VFI55_02675"
     CDS             complement(3455..4081)
                     /locus_tag="VFI55_02675"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET7072972.1"
                     /translation="MEATFEVLSEEELSRVTATYAPLTEAVRELIDATVRTHADDDLI
                     RQARTAIDAVTQSLLSQQTRPPGVSYRIDGRPLPLGNAATGQCNPIAPPLVVHHDGDG
                     RCWSEFVLGAAYEGPPGLVHGGVCALVLDHMLGEAASQGLTKPLFTGTITIKYLRGTP
                     LTSLRAEAFVERNEGFKAFARGYLSDAAGVTVEAEGVFIKPAWAREAE"
     gene            complement(4098..7106)
                     /locus_tag="VFI55_02680"
     CDS             complement(4098..7106)
                     /locus_tag="VFI55_02680"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HET7072973.1"
                     /translation="MIVTKPATERPRLPSVGRLGLIDPPAGKRLAQLGWYDHDDQAHV
                     DLLWSLSRAPDPDAALRALVRLAENPETGWNELNAALLTERGLRGRLFAVLGSSLALG
                     DHLAAHPRSWKLLRGKVKLPSPDELCASFTACCDSIEKQESAAPGAGPDTAVSRLRTL
                     YRDHLLVLAALDVAPTVEDEPVLPFTLVAEHLSDIADAALAAALRLAETTVCGDRTPP
                     RLAVIAMGKCGARELNYVSDVDVIFVAERADAASARVASEMMRVASEAFFQVDAGLRP
                     EGRNGELVRTVESHVAYYQRWAKTWEFQALLKARTAVGDAELGERYLAALMPMVWIAC
                     EREDFVVEVQAMRRRVEQLVPAEVRAREIKLGSGGLRDVEFAVQLLQLVHGRGDESLH
                     VASTVDALAALGRGGYIGREDTANLTASYEFLRLLEHRLQLQRLKRTHLLPEVDNEEA
                     VRWLARAAHIRPDGRHDAAGVLREELRHQNVRVSQLHAKLFYQPLLESIGPTGLEISH
                     GMTSEAAARQLAALGYEGPQTALKHMSALVNQTGRRGRVQSVLLPRLLNWMSYAPDPD
                     GGLLAYRRLSEALAGETWYLSTLRDKPAVAKRLMHVLGTSAYVPDLLMRAPRVIQDYG
                     DGPGGPKLLETEPEVVARALVASASRHPDPVRAIAAARALRRRELARIGSADLLGMLD
                     VIDVCKALTSIWVAVLQAALEAMIRANLPEDGKAPAAIAVIGMGRLGGAELGYGSDAD
                     VMVVCEPAHGVEDSRAVRWSTTIGEQVRALLGTPSVDPPLQVDANLRPEGRNGPMVRT
                     LSSYAAYYQQWAQPWEIQALLRAHAVAGDAELGQRFLLMADKTRYPADGVSAEAVREI
                     RRIKARVESERLPRGADPNTHTKLGRGGLADVEWTVQLLQLRYAHEVPALHNTSTLES
                     LDAAAAAGLISEEDVELLRHAWLTATRARNALVLVRGKPTDQLPGPGRQLNAVAVAAG
                     WQTDDGSEFLDNYLRVTRRAKAVVRKVFGS"
     gene            complement(7158..8498)
                     /gene="glnA"
                     /locus_tag="VFI55_02685"
     CDS             complement(7158..8498)
                     /gene="glnA"
                     /locus_tag="VFI55_02685"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET7072974.1"
                     /translation="MDRQKEFVLRTLEERDIRFVRLWFTDVLGYLKSVAIAPAELEGA
                     FEEGIGFDGSSIEGFARVSESDTVANPDPSTFQVLPWATNSGHHHSARMFCDITMPDG
                     SPSWADPRHVLRRQLQKANDLGFSCYVHPEIEFFLLKPGPDDGSRPVPVDNAGYFDQA
                     VHDSASNFRRHAIEALEFMGISVEFSHHEGAPGQQEIDLRFADALSMADNVMTFRYVI
                     KEVAIDNGARASFMPKPFAEHPGSAMHTHMSLFEGDVNAFHSADDPLQLSDVGKSFIA
                     GILEHASEISAVTNQWVNSYKRLVVGGEAPTAASWGAANRSALVRVPMYTPHKTSSRR
                     IEVRSPDSACNPYLAFAVLLAAGLRGVEKGYVLGPQAEDNVWDLTPEERLTMGYRELP
                     TSLDSALRSMESSELVAETLGEHVFDFFLRNKRTEWANYRSHVTPYELSTYLSL"
     gene            complement(8591..10150)
                     /locus_tag="VFI55_02690"
     CDS             complement(8591..10150)
                     /locus_tag="VFI55_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7072975.1"
                     /translation="MTRMLRLRPLSSALLSFGLLAGQSALPVAGATPEPGAGQTPSPA
                     PPSAVVRGQDWGSCSHFVTDTSDVPTAQCTTVSVPVDYANPGGAQAKLAVIRVPATGQ
                     RMGSLFINPGGPGASAVDMVVGLAPDLANSAITRNFDLVGFDPRGVGHSTPQLRCRTD
                     AERDAYRREPMVDYSPSGVAHIEQLYRQMAQQCVDRMGNAFLANVGTASGARDMDMVR
                     QALGDDQINYLGYSYGTELGTAYLEGFSNHVRAMVLDGAIDPTVAPVDENVNQMAGFQ
                     TAFNDYAADCARSAACPLGTDPTQFVNRYHALIDPLVTKPGRTSDRRGLSYADATTGT
                     INALYTPQHWKYLTSGLLGLQRGTDAGDLLLLADDYEDRDKNGHYGNDQDTFNAVRCV
                     DAPTPTDSATWVSADRRIRQAAPFLSYGQCTGDAPRDLCALWPVPATSRPHAAPTVAP
                     GKVVVVSTTHDPATPYQSGVSLARQLGAPLITYDGTQHTAVFNGDACVDTAVMRYFVA
                     GAVPGALNCRP"
     gene            complement(10321..11877)
                     /locus_tag="VFI55_02695"
     CDS             complement(10321..11877)
                     /locus_tag="VFI55_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7072976.1"
                     /translation="MCLSRRDKIARTLLIWTAIAAVALLLAGCLRVVGGRAVMAGPKL
                     GQPIEWTPCRSSNPQAKIPDGAMCGRLAVPVDYDHLDGDVAALAMIRFPATGDKIGSL
                     VINPGGPGESGIEAALGVVQSLPKRIRERFDLVGFDPRGVGSSRPAIWCNSDADNDRL
                     RTEPDVDYSPAGVAHIEDETKQFVGRCVDKMGKSFLANVGTVNVARDLDAIRAALGDD
                     KLTYLGYSYGTRIGSAYAEAYPKNVRAMILDGAVDPNADPIEADLRQAKGFQDAFNDY
                     ATDCAKTPTCPLGTDPTKAVDVYHSLVDPMVDPNNEMIGRPVPTKDPRGLSYSDAIVG
                     TIMALYSPSLWHHLTDGLSELVDHRGDTLLALADMYMRRDPNGHYTNATDARVAINCV
                     DQPPITDRAKVIDEDRRSREIAPFMSYGQFTGDAPLGTCAFWPVPPTSKPHAVSAPGL
                     APTVVVSTTHDPATPYKAGVELANQLRGSLLTFDGTQHTVVFQGDSCIDDYVTEYLLG
                     GATPPSGAKC"
     gene            complement(11935..13326)
                     /locus_tag="VFI55_02700"
     CDS             complement(11935..13326)
                     /locus_tag="VFI55_02700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HET7072977.1"
                     /translation="MQRLSGLDASFLYLETPSQPMHVCSIMELDTSTMPGGYAFDRLR
                     EALTLRMRAMPQFREKLANSPLNLDHPVWVDDENFDVDRHLHRIGLPPPGGRSELSEI
                     CGHIASLPLDRRRPLWEMWVIEGVAGTDCHRDGRLAVMTKVHHSGVDGVTGANLMSQL
                     CATEADPPAPDPVEGVGGGGGWQIAAGGLFRFAARPLHLANMVPQTMSSVVTTLQKAR
                     EGKAMARPFAAPRTAFNASISGRRTIAYAQLDLEDVKRVKDHFGVKVNDVVMALVSGV
                     LRQYLVDRDALPEAALVASVPVSVHGKSDRPGRNQVSAMFSSLHTQIADPVERLKAIA
                     EANSIAKQHSSAIGASLLQDWSQFAAPAVFGIAMRLYARTRLTKSMPVYNLVVSNVPG
                     PQVPLYMLGCQVKAMYPLGPIFHGSGLNITVMSLSGKLDVGLISCPELLPDLWAMADD
                     FAVAMEELLATCG"
     gene            13476..14333
                     /gene="panB"
                     /locus_tag="VFI55_02705"
     CDS             13476..14333
                     /gene="panB"
                     /locus_tag="VFI55_02705"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET7072978.1"
                     /translation="MSEQNIYGADLSAGTSMPAKPKPKIRTHHLQKMKAEGHKWAMLT
                     VYDYSTSRILDDAGIPVLLVGDSAANVVYGYDTTVPVSIDELIPLVRGVARGSHHAMV
                     VADLPFGSYEAGPGAALAAATRFMKEGGAHAVKLEGGERVADQIACLTTAGIPVMAHI
                     GFTPQSVNSLGGFRVQGRGDAAERTVADAIAVAEAGAFSVVMEMVPAELATQITGKLT
                     IPTIGIGAGPNCDAQVLVWQDMAGLSGGKPARFVKRFADIGGELRRAAMQYAEEVAGG
                     VFPADEHCF"
     gene            14486..16015
                     /locus_tag="VFI55_02710"
     CDS             14486..16015
                     /locus_tag="VFI55_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HET7072979.1"
                     /translation="MPVKAQKTSRHLEVERKFDVVDSTVSPSFEGIAAVTRVDKPPTQ
                     SLDATYFDTPTQDLARNKITLRRRTGGSDAGWHLKLPAGPDARTEIHAPLTSGTAEHD
                     TVPSELRDVVLAIVRSKSLEPVARITTERQTQVLYGAEGTALAEFSNDHVTAWSASAS
                     DNPGAQPAEQEWREWELELIKGTESKETNGTADTELLSRLSNRLLDAGAAPAGHASKL
                     ARVLGATAQSKAAEPPADPVHRAVKEQIDELLLWDRAVRADAYDAVHQMRVTIRKVRS
                     LLADSHESFGSSDDAGVLDELRELSNVLGAARDAEVLAERYQLALDKLPPELVRGPVR
                     ERLVEGARHRYQSGLRRSLIAMRSQRYFRLLDALDAWVANTTASGEEAAPITIDAAYK
                     KVRKAAKAAAEVASGDEQEHDDALHRIRKRAKRLRYTAAATGATRVSEQAKVIQTLLG
                     DHQDSVVSREHLSHQAEAAHAGGEDTFTYGLLYQQENDLAEQCSQQLDGALRKLAKSV
                     H"
     gene            complement(16069..16495)
                     /gene="rnpB"
                     /locus_tag="VFI55_02715"
     ncRNA           complement(16069..16495)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFI55_02715"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            16650..17828
                     /locus_tag="VFI55_02720"
     CDS             16650..17828
                     /locus_tag="VFI55_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7072980.1"
                     /translation="MTETEGFVDQAVMGLAEPQPMYKALRESAPVFRAPQAVVLSRLA
                     DIEMALKHTELFSSNMDAVDLGNIRPLIPLQVDPPEHAKYRRILDPLFTPREMARREP
                     RVTELVNEMIDRFADRGECDFHTEFAVPLPCTVFLQLLGLPLGDLDKFLEWKDGVIRP
                     EGESGYDRRHETSAPVALEIYSYFERAIDDHIADPRDDVLSAMIAANFKGDGGQPLSR
                     EELLDICFLFLIAGLDTVTDSLDCFFVYLARHPEHRHQLIEQPDILPGAIEELLRWET
                     PVPGVARVAMQDVEVGGCPISKGERVSPLLGAANTDPAEFPDPDVVDFARNPNRHRAF
                     GGGPHRCLGSHLARMELRVALREFHRRIPDYEIKPGTELTYTAALRSVESLPLIFAAP
                     "
     gene            17825..18019
                     /locus_tag="VFI55_02725"
     CDS             17825..18019
                     /locus_tag="VFI55_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET7072981.1"
                     /translation="MTRVSVDADRCTGHGRCYTLAPTVFDADEAGHCIVLTEDVSGER
                     EAQAVIGEQNCPEQAITLSN"
     gene            complement(18076..18678)
                     /locus_tag="VFI55_02730"
     CDS             complement(18076..18678)
                     /locus_tag="VFI55_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HET7072982.1"
                     /translation="MQPSRPPGESAGDVPADIRAQVMSAVLDELARWGVERFSVEAMA
                     ERHHLDTAMIYRYWGDRQRLIVDAAVADVEGSRAAADTGSLRGDLLALARAVTERLNT
                     EVGRTLLRAVVMDRPGHHDEETRTMFWAARFDVVRAVVDRARQRGELRDGVNTLAAVL
                     IVLAPLNIRALYSDVPVDDDYCLAIADMAWHALALHTDLQ"
     gene            complement(18894..20009)
                     /locus_tag="VFI55_02735"
     CDS             complement(18894..20009)
                     /locus_tag="VFI55_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional RNase H/acid phosphatase"
                     /protein_id="HET7072983.1"
                     /translation="MKVVIEADGGSRGNPGPAGYGAVVRTADRSAVLAEAKQAIGRAT
                     NNVAEYRGLIAGLDDAVRLGATEAEVFLDSKLVVEQMSGRWKVKHPDLIGLHAQARTL
                     ASRFDRISYAWIPRARNSHADRLANEAMDAAADVAGPAENSAPPVAEPAKSVGKQAET
                     GPGWTGARGTPTRLLLLRHGQTELSAQRRYSGRGNPALTEVGRQQADAAARYVAQRGG
                     IAAVLSSPLQRAYDTAAAAAKALGLDVSVDDDLIETDFGAWEGLTFAESAERDPELHR
                     RWLRDTGTTPPGGESFDEVLDRVLRVRDRIIAAHQGATVLVVSHVTPIKMLLRLALDA
                     GPGILYRLHLDLASLSIAEFYSDGATSVRLVNQTGYL"
     gene            complement(20006..20743)
                     /locus_tag="VFI55_02740"
     CDS             complement(20006..20743)
                     /locus_tag="VFI55_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET7072984.1"
                     /translation="MKAEVAQQLSLLELSKLDAELSRIAHRASHLPQQQAYERTRAEY
                     NAATDRLGVLRIALEDVDAQVSRFESEIEAVRQREDRDRSMLDAGATDARQLSDLQHE
                     LDTLLRRQTSLEDSLLEVMERREEMQAQLNSEQGGVETLQAELASAQQSLDAAVAEID
                     QARQEHSSRRDSLSATLDPALSALYERQRAGGGPGAGPLLGHRCGACRIEIGRGDLSR
                     ISAAADDELVRCPECGAILLRVKGFQQ"
     gene            complement(20740..>21028)
                     /locus_tag="VFI55_02745"
     CDS             complement(20740..>21028)
                     /locus_tag="VFI55_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET7072985.1"
                     /translation="CGGAGDSLLAAAVGADVQAYVTADLRHHPADEHRRASQVALIDV
                     AHWASEFPWCQQAAGILRSHFGAALPVRVCTIRTDPWNLGRPGHDYGRNES"
BASE COUNT         3513 a         7122 c         6898 g         3495 t
ORIGIN      
        1 cggtgaaccg ctcgtggtca atgaggtccg gtgtcggtgc gtccggaagg tctgggtacg
       61 tggacttcag gcgagccacc aggtcgaggt gagttacccg gtccatggga gtcgtcatat
      121 gagctccgct cgttcgtatg agtctgcgac ctccgagcgc ccccacggat tcccgattct
      181 ccccacggcg cagtgcagtg ctccaaccac agctcacccc aaccgtggcc ttcacgcaac
      241 cgacaaacgg gttaacggcg aactcagccc ggcaatccgg cggctacgac gacacggacg
      301 gagcactgct cccaggatct cggtttgctc cccatcgccg gtacatgtcg caggcgtcca
      361 ccgacgggtc gcgcttttcg aggattaatc cgctgcatcg cagaaaggcg gccatctcta
      421 tctgcttagc tagggccagg cgaccggcat cggttgcggc caggttgatc agaggatgaa
      481 ggagccagtc atgttcctct cagaaatcat ttcgcgccgg ttggtggtcg gcgcgatcgg
      541 cgcgggcgcg gtaaccggga cgctgctagg cgccgccgcg gggatcgccg cggccgatcc
      601 gccgccgcgg ccgcccaact gcacagccgc ggacgtagcc agtgtcgcgg ccggtgttgc
      661 cgcgtccctg tccacctacc tgttcactca cccggacgtg aacgggttct acaccggcct
      721 tcaggatcag cccaaagatc agctccgcga cgacgtgcag aactacttca acgccaaccc
      781 gcaagagcaa gccgaccttg agggcatccg tcagccgctg gttgacatca ggcaacgttg
      841 tcagtggact ccgttactcg ggcaggacgg caccacgccc tgaatgatcg ggccgcgcca
      901 tgtaaccacg tcccgaaacc cgcgagcgca aatccgcggt acgaattccg gtctcatcgt
      961 gcccacggcg gaatatactg cggccctcag ttcatgtaag ggctcaaaca agccccggcc
     1021 atcgcggcga aggagatcgc aatgaatacg tgggtgcggt cttggagcgc cgcggggata
     1081 gcggtgttgg ccatcacgtc gatccctgag ctgtatgccg tcattcagcc gcccggggtg
     1141 gccaacgccg acgtctgcgc gagcgtcggc aggcgtgttt cggtaagcgg ttgcaccaac
     1201 gtcgccgata ccgtcaacac ctatgccccg ccgccggcct actatgcgcc gctgccgcag
     1261 gactatccgc ccccaccgcc ggtaagtgtc tgcgtgggtg ccggccggcg catccacgta
     1321 agcgggtgct cctagcaacg gcgactgcgt gggcgtcagc cgactctgca agggaagcgc
     1381 ggcgccgcac ttgattgccg cgactttgcg ggttcgatca cggcgacgag agatccgcgc
     1441 gggatgcacg caaagtcggg gcaaagtcgt ggaacggcaa atcttttggg ccgcggttga
     1501 tctcgtgccg ccggcgcttg ttgtagccaa atgttaagac ttgcggctct tgcggcagct
     1561 gcgagttcgg cgatagcctt ccgcccatga tcacgagcaa caccgcggaa atgatggtcg
     1621 gtggcggcgg tggccgtctt ggtggcggcg gtggccgtct cggtggcggc ggtggccgtc
     1681 tcggtggcgg cggtggccgc ctcggtggcg gcggtggccg cctcggtggc ggcggtggcc
     1741 gcctcgtcgg tggcggcggc ggttagcccg aagcaaccaa ataccatttc ttcccgcgcg
     1801 gcccccgcgg tttcgatgcg caaggggcac gcgtaggtag agcgggattg ctcatcagcc
     1861 ggttcgacgt cggcgccgat cgggctgctc aagcacgccc gttgcgagcg tggtgctgac
     1921 gggcgcggtc agttaccgat tgcgccacaa tcggtctcgc aagcgtctca acggcgtcga
     1981 tcagatgatt gggccgggcg gttggttctg gtcggcggtg ccgcgcagtt cgagttgcat
     2041 ccaggcaacg tcatgccagc tgtcgtgttt ccattcgacg cgccgataca gcccgatgcg
     2101 ctcaaacccg aacgaccgat ggaaactcgt gctggcctcg ttgggttggg tgatgcccgc
     2161 gaaggcacgc cgatagccac gctccctgag gcggtccagc agccgcgtgt agagctggcg
     2221 gccgcagccg gcgcgatgat ggtcgacact gacgtagatc ccggtctcag tcgaccactt
     2281 gaatgcgggc tggcgattga acgcgtgccc gtacgcgaag ccgatgatct cctcatcgcg
     2341 ttcgaggacg agccattcgt gggcctcgcg ggccgcggcg atgcgcgcag ccatctcatc
     2401 gaccgaaggg acctccagct cccaactgat caccgtgccc aggacatacg ggcggtaaat
     2461 cgcgacgcaa gcgtccgcat cctgtcgaga cgccggacgc acaaggctgg tgctcgtttg
     2521 catgcccgca ttcttccagt cgctgtcggg ggtttgcgcg agtgtggccg actgagcaca
     2581 tgggccagag tgcggcctca gccgtcgagc cccaagtcct tgcggaaacg ctgcatgccg
     2641 tcgatcttgt cggccagcgt ggcgtccggg ccggcgtaga acatccacgg catggtgatg
     2701 atgccggtga taccggcggc tgcggcgcgt tgatagtcgg cgccggtgaa ggcatcggtc
     2761 aatggcgtca gaaccgtgaa atccgttaac gagagcccgt tttcgatccg cagttcccgc
     2821 agccggccaa ccgcttcgat ggcgcgatcg gtcgtgatca gatcaccgat ccagccgtcg
     2881 ttgcgggcag cacggcgcag cgcggcgtcg ctgaggccgc cgacgtacac cggtatcggc
     2941 ggaggggtgg gctgcatctc cagccgtggt gtcgggtaga actcgccgtc gaactccgtc
     3001 caacccggtt cccacagcgc ccgcatcaac tcgatgatct cgtcggtgcg tttcccgcgg
     3061 gcgtcgaatt gctcgcccat cagggcgaat tcctctcggc accagccgac tccgatgccc
     3121 agctccaccc ggcccgccgc caaaaccgcc gcggtgccaa tggctttggc cgccgagtag
     3181 gggttgcgca tggccgggat gtagacggtg gtgacgaagc gcagccggtc ggttacctgg
     3241 gccagcgctc cgacgagaac ccagggatcg ggccaatcgg tgaagggctg ccaccgtcgt
     3301 tgcccgtctt tggtgtatgg gtacggggtg tccaggttct ccaggttgac gacgtggtcg
     3361 gggattccga ttccgtcgta gcccagctcg tcggcggcct tggcgatctc gatgatctcc
     3421 cgagtgttca aaaaggcgct gctgacgcag aacttcattc ggcctcgcgc gcccacgccg
     3481 gcttgatgaa taccccttcc gcctctaccg tgacgcccgc ggcatccgaa aggtaacccc
     3541 gcgcaaaggc tttgaagcct tcattgcgtt cgacgaatgc ctcggcgcga agcgacgtca
     3601 gtggcgtgcc acgcaggtat ttgatggtga tggttccagt gaacagcggt ttggtcaggc
     3661 cttggctggc cgcctcgccc agcatgtggt ctaagaccag ggcacaaaca ccgccgtgca
     3721 ccaggccggg aggaccttcg taggccgcgc ccagaacgaa ctcgctccag caccggccgt
     3781 ccccgtcgtg gtggaccacc aaaggtgggg cgatcggatt gcactggccg gtcgccgcgt
     3841 taccaagcgg gagtggacgg ccgtcgatgc ggtagctcac gccgggtggc cgggtctgtt
     3901 gactgagcag cgattgggtc accgcatcga tcgcggtccg cgcctgccga atgaggtcgt
     3961 cgtcggcgtg ggtccggacg gtggcgtcga tgagctcgcg aacagcctcc gtcaacggcg
     4021 catacgtcgc cgtcacccgg ctgagctcct cttcgctcaa gacctcaaac gtggcttcca
     4081 acgatccgcc ttccgactca gctcccgaac accttgcgca ccaccgcttt cgctcgccgg
     4141 gtgacccgca gatagttgtc caggaactcg ctgccgtcgt cggtctgcca gccggcggcg
     4201 accgcgaccg cgttgagctg acgcccgggc cccggcagct ggtcggtggg cttgccacgg
     4261 accagcacca gcgcgttgcg agcccgggtg gccgtcagcc aggcgtggcg cagcagctcc
     4321 acgtcttctt cggagatcag gccggccgcg gcggccgcgt ccagagattc cagcgtcgag
     4381 gtgttgtgca gggccggcac ctcgtgcgcg taccgcagtt gcaggagctg cacggtccat
     4441 tcgacgtcgg ccaatccccc gcgacccagc ttggtgtggg tgttggggtc ggcgccacgc
     4501 ggcaaccgct cggactcgac gcgggccttg atgcggcgga tctctcgcac cgcctcggcc
     4561 gacaccccat cggctggata gcgcgtcttg tcggccatca gcaagaaccg ctgacccagc
     4621 tccgcgtcgc ccgcgaccgc gtgggcgcgc agcagggcct ggatctccca cggctgcgcc
     4681 cactgctggt agtacgcggc gtacgagctc agcgtgcgga ccatcggacc gttgcgtccc
     4741 tccggccgca ggttggcgtc gacttgcagc ggcgggtcga cgctgggggt ccccagcaat
     4801 gcccgaacct gctcgccgat cgtggtcgac caccgcaccg cccgcgaatc ctcgacaccg
     4861 tgggccggct cgcagaccac catcacatcg gcatcggacc cgtagccgag ctcggcgccg
     4921 cccagcctgc ccatgccgat aacggcgatg gccgcgggcg ccttgccgtc ttcggggagg
     4981 ttggcgcgga tcatcgcctc caacgcggct tgcagcacgg ccacccatat ggaggtgagc
     5041 gccttgcaga cgtcgatgac gtcgagcatg ccgagcagat cggcggaacc gatgcgggcc
     5101 agctcgcgac ggcgcagcgc acgcgcggcg gcgatcgccc gcaccgggtc cgggtgacgg
     5161 ctcgccgagg cgaccagagc acgggccacc acctcgggtt cggtctcgag cagtttcggg
     5221 ccgcccggtc cgtcgccgta atcctgaatg accctgggcg cccgcatcag caggtcgggc
     5281 acgtaggcgc tggtccccag cacatgcatg agccgcttgg ccaccgcggg cttgtcccga
     5341 agcgtggaca ggtaccaggt ttcgccggcc agcgcctcgg acaggcgccg ataggccaac
     5401 aggccgccgt cggggtcggg cgcatacgac atccagttca gcagcctggg cagcagcacc
     5461 gactgcaccc gaccgcgccg accggtctgg ttgaccagcg ccgacatgtg cttcaacgcc
     5521 gtctgtggac cctcgtaacc cagcgcggcc agctgtcgcg ccgcggcctc cgaggtcatg
     5581 ccgtgcgaga tttccaggcc ggtggggccg atcgattcca gcagcggctg atagaacagc
     5641 ttggcgtgca gctgggacac ccgcacgttc tggtgccgca gctcctcgcg cagcaccccc
     5701 gccgcatcgt gtcggccgtc cggccggata tgggccgccc gcgccagcca gcgcaccgcc
     5761 tcctcgttgt cgacctccgg cagcagatga gtgcgcttga gccgctgcag ctgtagccgg
     5821 tgttcgagca gtcgcaggaa ctcgtacgac gcagtgaggt tggccgtgtc ctcgcgcccg
     5881 atgtagccgc cgcggcccag cgcggccagc gcgtccaccg tcgaagccac gtgcagcgac
     5941 tcgtcgccgc ggccgtgcac caactgcagc agctgcacgg cgaactccac gtcgcgcaaa
     6001 ccaccgctgc cgagtttgat ctcgcgggcg cggacctcgg ccggcaccag ctgctctacc
     6061 cggcggcgca tggcctgcac ctcgaccacg aagtcctcgc gctcgcaggc aatccacacc
     6121 atcggcatca gcgctgccag gtagcgctcg ccgagttccg cgtcgccgac cgccgtccgg
     6181 gccttcagca gggcctgaaa ctcccaggtc ttcgcccagc gctggtagta ggcgacgtgc
     6241 gattcgacgg tacgcaccag ttcgccgttg cggccttcgg gcctcaggcc ggcgtccacc
     6301 tggaagaacg cttcggaggc cacccgcatc atctcgctgg cgactcgagc gctggccgcg
     6361 tcggcgcgct cagcgacgaa gatgacgtcg acgtcgctga cgtagttcag ttcacgggca
     6421 ccgcatttgc ccatcgcgat gaccgccagc cgtgggggcg tgcgatcccc gcacacggtt
     6481 gtctcggcga gccgcagcgc ggccgccagc gccgcgtccg cgatgtccga caggtgttcg
     6541 gccaccaggg tgaacggcag aactggctcg tcctcgaccg tcggggcgac gtcgagcgcg
     6601 gccagcacca gcagatggtc acggtacaac gtgcgcaatc gcgacaccgc cgtgtccggc
     6661 cctgcgccgg gcgcggctga ctcttgcttc tcgatgctgt cacagcaagc ggtgaacgac
     6721 gcgcacagct cgtcggggga cggcagtttg accttgcccc gcaacagctt ccacgaccgc
     6781 gggtgggcgg ccaggtgatc ccccagggcc agcgacgaac ccagcacagc gaacagtcgc
     6841 ccacgcaggc cgcgttcggt gagcagcgca gcgttgagct cgttccatcc ggtctcgggg
     6901 ttctccgcca gccggaccag ggcgcgcagc gcggcgtcgg ggtcgggcgc acgcgacagc
     6961 gaccacagca gatcgacgtg cgcctggtcg tcgtggtcgt accaacccag ctgcgccaga
     7021 cgcttgcccg ccggggggtc gatcaacccg aggcggccga cgctgggcag ccgggggcgc
     7081 tccgtcgcgg gtttggtcac gatcaagacg gtatcgcaac ccgccgatcc ctcagaccaa
     7141 tcggctcgcg atgggcgcta cagcgacagg taggtactca gctcgtacgg cgtgacatga
     7201 ctgcggtagt tcgcccactc cgtgcgcttg ttgcgcagga agaagtcgaa cacgtgctcg
     7261 cccagggttt ccgcgaccag ctcggaggac tccatgctgc gaagcgcgct gtcgagactg
     7321 gtcggcagct cgcgataccc catcgtgagg cgctcctcgg gcgtgaggtc ccagacattg
     7381 tcctcggcct gcggccccag cacgtagccc ttctccaccc cgcgcagccc ggcggccagc
     7441 aacacggcga acgccaggta gggattgcac gccgagtcgg ggctgcgtac ctcgattcgc
     7501 cgcgacgacg tcttgtgcgg tgtgtacatc ggcacccgca ccaaagccga ccggttggcc
     7561 gccccccacg atgcggccgt gggcgcctcg ccgccgacca ccagccgctt gtaggagttg
     7621 acccattgat tggtgaccgc gctgatctcg gaggcgtgct cgaggatccc ggcgatgaac
     7681 gacttgccca cgtccgacaa ctgcagcgga tcgtcggcgc tgtggaaggc gttgacgtcg
     7741 ccctcgaaca ggctcatatg ggtgtgcatc gccgagccgg ggtgttccgc gaacggcttg
     7801 ggcatgaatg acgcccgagc gccgttgtcg atcgcgactt ccttgatgac gtagcggaag
     7861 gtcatcacgt tgtcggccat cgacaacgcg tcggcgaagc gcaggtcgat ctcctgctgg
     7921 ccgggcgcgc cctcgtggtg gctgaactcg accgagatgc ccatgaattc cagggcctcg
     7981 atggcgtgcc ggcggaagtt ggacgccgag tcgtgcaccg cttggtcgaa atagccggcg
     8041 ttgtcgacgg gaacgggcct cgacccgtcg tcgggaccgg gcttgagcag gaagaattcg
     8101 atttcgggat gcacatagca ggagaagccg aggtcgttgg ccttctgcag ctgacggcgc
     8161 agcacatgcc gcgggtcggc ccacgacggc gagccgtccg gcatggtgat gtcgcagaac
     8221 atccgcgccg agtggtggtg gccggaattc gtggcccagg gcagcacctg gaacgtcgac
     8281 ggatccgggt tggccacggt gtcggattcc gagacccgcg cgaagccctc gatcgaggaa
     8341 ccgtcgaagc cgatgccctc ttcgaaggca ccttccagct cggcgggggc gatggcgacc
     8401 gacttgaggt agccgagcac atccgtgaac cagagccgca caaagcggat gtctcgttcc
     8461 tccagcgtgc ggaggacgaa ttccttctgt cgatccatat cccgaacagt aggcaacagc
     8521 tgttaaatct gtgttaccga tgcccgctca gaaccattgc gcagtgcaaa ttagagccgc
     8581 tcaactgggc ctagggacgg cagttgaggg cgcccggcac cgctcccgcg acgaaatagc
     8641 gcatcaccgc ggtgtccaca cacgcgtcgc cattgaacac cgcggtgtgt tgggttccgt
     8701 cgtacgtgat cagcggggcg ccgagctggc gggccaggct cacgccggac tggtacgggg
     8761 tggccgggtc atgcgtggtg gaaacgacga cgaccttgcc cggtgcgacg gtcggggcgg
     8821 cgtgcggtcg cgacgtggcg ggcaccggcc atagcgcgca caggtcgcgc ggcgcatcgc
     8881 cggtgcactg cccgtagctc aagaacggag cggcctggcg gattcgtcga tcggcggata
     8941 cccacgtcgc ggagtccgtc ggcgtcggcg cgtcgacgca acgcacggcg ttgaacgtgt
     9001 cctggtcgtt gccgtagtgg ccgtttttat cacggtcttc gtaatcgtcg gcgagcaaca
     9061 acaagtcgcc ggcgtcggtg ccgcgctgca gcccgagcag accgctggtc aagtacttcc
     9121 agtgctgcgg ggtgtaaagc gcgttgatgg tgccggtggt cgcgtcggcg tagctcaggc
     9181 cgcgccgatc cgacgtccgg ccgggcttgg tgaccagcgg gtcgatcagg gcgtggtacc
     9241 ggttgacgaa ttgagtcggg tcggtgccca gcgggcaggc cgccgagcgc gcgcagtccg
     9301 cggcgtaatc gttgaaagcc gtttggaatc cagccatttg gttgacgttc tcgtcgactg
     9361 gggccacggt cgggtcgatg gcgccgtcga gcaccatcgc ccgtacgtgg ttactgaatc
     9421 cctcgagata ggcggtgccc aattcggtgc cgtagctgta gcccaggtag ttgatctgat
     9481 cgtcgcccag ggcctggcgg accatgtcca tgtcgcgcgc accggatgcg gtgccgacgt
     9541 tggccaggaa cgcatttccc atccggtcga cgcattgctg ggccatctgc cggtagagct
     9601 gctcgatgtg cgcgacgccg gaggggctgt aatcgaccat cggttcacgc cggtaggcat
     9661 cgcgttcggc gtcggtacga caccgcaact gcggggtcga gtggcccacc ccgcgggggt
     9721 cgaaccccac caggtcgaag ttgcgggtga tggcgctgtt cgccaggtct ggcgccaagc
     9781 cgaccaccat gtcgaccgcg gacgccccgg gtccgcccgg attgatgaac agcgatccca
     9841 tccgctggcc ggtcgccggt acccggatca cggccagctt cgcttgcgcc ccaccggggt
     9901 tggcgtagtc gaccgggacg gacacggtgg tgcattgggc ggtgggaacg tcgctggtgt
     9961 cggtcacgaa atggctgcaa ctgccccagt cctgcccccg aacgacagca gacggcggag
    10021 ccgggctcgg ggtctggccg gcaccgggtt ccggagttgc tccggcgacc ggcagagccg
    10081 attgccccgc aagcagtccg aacgacagca gcgccgagct caacggtctc aggcgcaaca
    10141 tgcgtgtcat cgttgcacct tcgaataccc gtggcggcgc gaaacggtca cgccttgatc
    10201 tcgggcaccg ccggattgcg tggctgttag cctcgcggca cgcttaccaa attgcccgcc
    10261 ttgccccctc gccgcggcgc ggctgggggt gcccccagcg cggggcgcat cgtcggcggg
    10321 ctagcacttc gcgccgctcg gcggcgtggc gccaccgagc agatactccg tcacgtagtc
    10381 gtcaatgcag ctgtcgccct ggaacacaac cgtgtgctgg gtgccgtcga aggtaagcag
    10441 cgacccgcgc agctgattcg ccagttccac cccggcctta tacggcgtcg ccggatcatg
    10501 cgtggtcgac accaccacgg tcggggccag gccgggcgcc gagacggcgt gcggcttgct
    10561 cgtgggcggc accggccaga acgcacaggt gcccagcggc gcgtcaccgg tgaactgtcc
    10621 gtagctcatg aacggcgcga tctcgcgcga gcggcggtct tcgtcaataa ccttggcgcg
    10681 gtctgtaatc ggcggctggt caacacagtt gatcgccact cgcgcgtcgg tggcattggt
    10741 gtagtgaccg ttcgggtcgc ggcgcatgta catatcggcc agggcaagca gggtgtcgcc
    10801 gcggtggtcg acgagttcgc tcaggccgtc ggtcaggtgg tgccacaagc ttggcgagta
    10861 aagcgccatg atggtgccca caatggcgtc gctgtaactc aacccgcgtg ggtccttcgt
    10921 gggtaccggc cgaccgatca tctcattgtt cgggtcgacc atcgggtcga ccaggctgtg
    10981 gtacacgtcg acggctttgg tcgggtcggt gcccagcggg cacgtcgggg tcttcgcgca
    11041 atcggtggcg tagtcgttga acgcgtcctg aaatcccttg gcctgacgca ggtctgcctc
    11101 gatggggtcg gcattgggat ccacggcccc gtcgaggatc atcgcccgca cgttcttggg
    11161 gtaggcctcc gcgtaggccg agccgatgcg ggtgccgtac gagtagccga ggtaggtcag
    11221 cttgtcgtcg cccagcgccg cgcggatcgc atccaggtcc cgggcaacgt tgacggtgcc
    11281 cacgttcgcc agaaagctct tgcccatctt gtcgacgcag cgaccgacga attgcttggt
    11341 ctcgtcctcg atgtgggcca cacccgccgg gctgtaatcg acatcaggtt cggtgcgcaa
    11401 ccggtcgttg tcggcatccg agttgcacca gatcgccggt cgcgacgaac ccaccccgcg
    11461 ggggtcgaag ccgaccaggt cgaaccgttc gcggattcgc ttgggcaacg actgaacgac
    11521 acccaacgcg gcctcgatgc ctgactcgcc aggcccgccc ggattgatca ccagcgaacc
    11581 gatcttgtcg cccgtcgccg ggaaacggat catcgccaat gccgccacgt cgccgtcgag
    11641 gtggtcgtaa tcgacgggca cggccagcct tccgcacatt gcgccatcgg gaatcttggc
    11701 ctgcgggttg gaagagcggc acggtgtcca ttcgatcggc tggcccagtt tgggtcccgc
    11761 catgactgcg cgtccgccga cgacgcggag gcagccggcg agcaggagcg ccacggcagc
    11821 gatcgccgtc cagatcagca gcgtgcgcgc gattttgtcg cggcgagaca ggcacatgcc
    11881 accatatgct gccagagcca acctgagatt ctgatgagcg gcaccgccgg ctaactaccc
    11941 gcaggtcgcg agcagttctt ccatcgccac ggcgaagtcg tcggccatcg cccacaggtc
    12001 cggcagcaat tccgggcacg agatcagccc gacgtctaac ttgccgctca gcgacatcac
    12061 ggtgatgttc aggcccgaac cgtggaagat cggccccagc ggatacatcg ccttgacctg
    12121 gcagcccagc atgtacagcg gcacctgggg gcccgggacg ttggagacca ccaggttgta
    12181 cacgggcatg ctcttggtca acctcgtccg ggcgtacagc cgcatcgcga tgccgaacac
    12241 ggccggcgcg gcgaattgcg accagtcctg cagcagtgac gcgccgatgg ccgaactatg
    12301 ttgcttggca atcgaattcg cttcggcgat ggctttcagc cgctccaccg ggtcggcgat
    12361 ttgagtatgc aggctggaaa acatggcgga aacctggtta cggccggggc gatcggactt
    12421 gccgtgtacc gataccggca ccgacgccac cagcgccgcc tcgggtaagg cgtcgcggtc
    12481 gaccaggtac tggcgaagca ctccggacac caaagccatc accacgtcgt tgaccttgac
    12541 gccgaaatgg tccttcaccc ttttgacgtc ctcgaggtcg agttgggcgt acgcgatggt
    12601 gcgccggccg ctgatgctcg cattgaaagc ggtccggggt gcggcgaacg gacgcgccat
    12661 cgccttgccc tcgcgggcct tctgcagagt tgtgaccacc gaggacatcg tctggggcac
    12721 catgttggcc aggtgtagtg gccgggcggc gaacctgaac agtccgccgg cggcgatctg
    12781 ccatccgccg ccgccgccaa cgccttccac cggatccggc gcgggcgggt cggcttcggt
    12841 ggcgcacagc tgtgacatca ggttggcgcc ggtcaccccg tccaccccgg aatggtgcac
    12901 cttggtcatc accgccagcc gcccgtcgcg atgacaatcg gtgcctgcca ccccttcgat
    12961 gacccacatc tcccataacg gccggcggcg gtccaacggc agcgatgcga tgtggccgca
    13021 gatttccgac aattcgctcc ggccccccgg cggcggcaac ccgatgcggt gcaaatgccg
    13081 atcgacatcg aagttctcgt cgtccaccca caccgggtgg tcaaggttca gcggactgtt
    13141 ggccaacttc tcgcggaact gcggcatcgc cctcatgcgc agcgtcaacg cctcgcgcag
    13201 ccggtcgaac gcgtagccgc caggcatcgt cgacgtgtcc aactccatga tcgagcaaac
    13261 atgcatcggc tgtgagggag tttccagata gaggaaactg gcgtcgaggc cgctgagccg
    13321 ctgcatgcgc agaatcgtat gtctcacggc gcaccggcgg tgcaactgtg cagaagagat
    13381 ggcagaatgt cgaaagtcac gcgaacccgg ggaccttttc aggacgagaa cctgagcggc
    13441 cacgaggatc gacccgacca ctaccaggga caatgatgtc tgagcagaac atttacggtg
    13501 cagatttgtc agcgggcacc tcgatgcccg caaagcccaa gccaaagatt cgcacacacc
    13561 acctgcagaa aatgaaggcc gaaggccaca agtgggccat gctcacggtc tacgactatt
    13621 cgacctcccg aattctcgac gatgccggca tcccggtcct gctggtcggc gactcggcgg
    13681 ccaacgtcgt ctacggctac gacaccacgg tgcccgtgtc gatcgacgaa ctgatcccgc
    13741 tggtccgcgg ggtggcgcgg gggtcgcacc acgcaatggt cgtggccgac ctgccgttcg
    13801 gcagttacga ggcgggaccg ggcgccgcgc tggccgccgc caccaggttc atgaaggaag
    13861 gcggtgcgca cgccgtcaag ctcgagggcg gcgagcgggt ggccgaccag atcgcctgcc
    13921 tgacgacggc gggcatcccc gtgatggcgc acatcggctt cacgccgcaa agcgtgaaca
    13981 gcctgggcgg cttccgcgta cagggccgcg gcgacgccgc ggagcgcacg gtcgccgacg
    14041 cgatcgcggt cgccgaagcc ggggcgtttt ccgtcgtgat ggagatggtg ccggccgaat
    14101 tggccaccca gatcactggc aaactgacca ttccgaccat cgggatcggc gccggaccaa
    14161 actgcgatgc ccaggtcttg gtgtggcagg acatggccgg cctgagcggc ggcaagcccg
    14221 ctcgcttcgt caagcggttt gccgacatcg ggggcgaatt gcgccgggcc gcaatgcaat
    14281 acgcagaaga agtggccggc ggggtttttc cggccgatga gcactgcttc tgagcgctca
    14341 ctgagcgcgc ctattgcacc accttgcaga tcggtagccc aaacctcagc tccgatcgcg
    14401 tcgccggtat ggcactctat gggtgcccat cgaaaccgat catgatctat aagaccgcgg
    14461 cgacgcgccg tcaacggagc cgaaaatgcc tgtaaaagcg caaaaaacct cacgccatct
    14521 ggaggtggag cgcaagttcg acgtcgtcga ctcgacagtg tcaccgtcct ttgaaggcat
    14581 cgccgcggtg actcgcgtcg acaagccacc gacgcaatcg ctggatgcga catacttcga
    14641 cacgccgacg caggacctgg cacgcaacaa gatcaccctg cggcgccgta ccggtggctc
    14701 cgacgcgggc tggcacctga aattgccggc cggtcccgac gcccgcaccg agatccatgc
    14761 accattgaca tccggcacgg ccgagcacga caccgtgccg agcgaattgc gcgacgtcgt
    14821 gctggcgatc gttcgctcca agtcgcttga accggtcgcg cgcatcacga ccgaacgcca
    14881 aacccaggtg ctctacggcg cggagggcac cgcgctggcc gagttcagca acgaccacgt
    14941 caccgcatgg tcggcttcgg cctcggacaa ccccggggca caacccgccg aacaggagtg
    15001 gcgggagtgg gaactcgagc tcataaaagg tactgagagt aaggaaacaa acgggaccgc
    15061 cgacaccgag ctgttgagcc ggttgagcaa tcggctgctc gatgcgggtg ccgcgcccgc
    15121 cggtcacgcc tcaaagctgg cccgggtact cggcgcgacc gcgcaatcca aggccgctga
    15181 gccgcccgcc gacccggtgc accgggcggt gaaggagcaa atcgacgagc tgctgctgtg
    15241 ggatcgggcg gttcgggccg acgcgtacga cgcggtgcac cagatgcggg tgaccatccg
    15301 caaggtacgc agcctgctgg ccgactccca cgaatcgttt ggatcgtctg acgatgcggg
    15361 ggtcctggac gagctgcggg agctgagcaa tgtgctgggc gcggcccgtg acgccgaggt
    15421 gctggccgag cgttaccagc tggcactgga caagctgcca ccggagctgg tgcgcggacc
    15481 cgtccgggag cgtctcgtcg agggtgcccg gcaccgctac cagagcggac tgcggcggtc
    15541 cctgatcgcg atgcgttcgc agcgatactt ccgtctgctc gacgccctcg acgcgtgggt
    15601 cgccaacacc accgcttccg gcgaggaggc ggccccgata accatcgacg ccgcctacaa
    15661 gaaggtccgc aaggccgcca aggcggccgc cgaggtagca tccggtgacg agcaggaaca
    15721 tgacgacgct ctgcatcgaa tccgcaagcg cgccaagcga cttcgctata ccgcggccgc
    15781 taccggtgcg acgcgggtgt ccgagcaggc caaggtcatc cagacgctgc tcggcgacca
    15841 ccaggacagc gtggtcagcc gggaacatct gagtcaccag gccgaagccg cgcacgccgg
    15901 cggcgaggac accttcacct acggcctgct gtaccagcag gagaacgact tggccgagca
    15961 atgcagtcag cagctggacg gggcgttgcg caaactcgcc aaatcggtgc actaagcgct
    16021 ccgccggccc gctcggcgac tatgcgtgtc gggcgggccg ggggcgggcg gacgagttgg
    16081 cctataagcc ggattctgtt ccccgccgct gcggcttaag caacggcggc ggagcgacga
    16141 ccatccatct ggacacaccg tcgccgggtg cctcgagcgg cctacccgca ggctcgggcg
    16201 agcaaccctc gggcgcctgc gcggccgcac tcccgtgcgg ccttcttggc cttgcttcgg
    16261 gtggggtttg ccgagccact ccggtcaccc ggaatgctgg tgcgctctta ccgcaccgtt
    16321 tcacccttac caccacgaag gtggcggttt gttttctgtg gcactttccc gcgagtcacc
    16381 tcggattgct gttagcaatc accctgctct gtgaagtccg gactttcctc gactcgacgc
    16441 cggcctctgg atctgacacc agcccgccgc gagccgcggc cgcccagcca actcgtccgc
    16501 aggaacacgg tactcgctgc agaatctcct ggccagcggc acgacgccgg ccgtacaccg
    16561 gggcgtcggc gcccgcttac cggcggccgg ctattgccca attaatccaa tcattgccta
    16621 gatagtgatg ttagtgtcac gctgaccgca tgacagaaac cgaaggcttc gtcgatcagg
    16681 cggtgatggg tctcgcggag ccgcagccga tgtacaaggc gttacgcgaa tcggccccgg
    16741 tgttccgtgc cccgcaggcg gtggttctga gccgcctcgc cgacatcgag atggcactca
    16801 agcacaccga gctgttctca tcgaacatgg acgccgtcga tctcggcaac atccgacctc
    16861 tcattccatt gcaagtcgat ccgcccgagc atgccaagta ccgccgcatc ctcgacccgc
    16921 tgttcacccc gcgggagatg gcccgacggg aaccgcgtgt caccgagctg gtcaacgaga
    16981 tgatcgaccg cttcgccgat cggggcgaat gcgacttcca caccgaattc gccgtcccgc
    17041 tgccgtgcac cgtcttcctg cagttgctgg gtctgccgtt gggcgacctg gacaagtttc
    17101 tcgagtggaa ggacggcgtg attcgcccgg aaggcgaatc cggctacgac cgccggcacg
    17161 agacctcggc acccgtggcc ctggagatct acagctactt cgagcgcgcg atcgacgacc
    17221 acatcgccga cccacgtgac gacgtgctgt ccgcgatgat cgctgcgaac ttcaaagggg
    17281 acggcggcca gccgttgtcg cgcgaggaac tgctcgacat ctgcttcctg ttcttaatcg
    17341 cggggctgga caccgtcacc gactcgctgg actgcttctt cgtgtacctg gcacggcatc
    17401 cagagcaccg gcaccagctc atcgagcagc ccgacatctt gccgggcgcg atcgaggaac
    17461 tactgcgctg ggagacgccg gtacccggtg tcgctcgggt cgccatgcag gacgtcgagg
    17521 tgggcgggtg tcccatcagc aagggcgaac gggtcagccc gctgctcggc gcggcgaaca
    17581 cggaccccgc cgagttcccc gatcccgacg tcgtggactt cgcccgcaac cccaaccggc
    17641 accgcgcgtt cggcggaggg ccgcaccgct gccttggatc gcacctggcc cgcatggagc
    17701 tgcgagtggc gttacgcgaa ttccaccggc gcataccgga ttacgagatc aagcccggca
    17761 ccgagctgac ctacaccgcc gcgctgcggt cggtggaatc gttgcccttg atattcgccg
    17821 cgccgtgacc agagtttccg tcgacgccga ccgctgcacc ggtcacggac gctgctacac
    17881 gttggcgcca acggtcttcg acgccgacga ggctggccac tgcatcgtgc tcacggagga
    17941 cgtctcgggt gagcgcgaag cgcaggccgt catcggcgag cagaactgtc ccgagcaggc
    18001 gatcactctc tcgaactgac gggcatttcg cctcggcgtg accgcgttgc ccgcttctcg
    18061 ctggctgttg cggatctatt gcaggtctgt gtgaagggcg agcgcatgcc acgccatgtc
    18121 ggcaatcgcc aagcaatagt cgtcgtcgac ggggacgtca gagtaaaggg cacgaatgtt
    18181 cagcggcgcc aacacaatca gcacagcagc cagcgtgttc acgccgtcac gcaactcgcc
    18241 acgctgcctg gcccgatcca ccaccgcacg caccacgtcg aaacgtgccg cccagaacat
    18301 cgttcgggtt tcttcgtcgt gatgcccggg acggtccatg accaccgccc gcagcagagt
    18361 gcgacccacc tccgtattga gccgttcggt cacggcgcga gccagcgcca agaggtctcc
    18421 gcgcaacgag ccggtgtccg cggccgcccg cgagccctcg acatcggcga cggccgcatc
    18481 gacgatgaga cgttgccgat cgccccagta gcggtagatc atcgccgtgt cgaggtggtg
    18541 gcgctcggcc attgcctcga cgctgaaccg ctcgacaccc cagcgggcca actcgtcgag
    18601 cactgccgac atcacctgcg ccctgatgtc cgcaggcacg tcgcctgcgg actcaccagg
    18661 tggtcgagac ggctgcaatc aatgactcct taacccctga cctcactacc cgccgcgatc
    18721 gctcgccgaa acgcggctgg cgcaattgcc tcctcccggc acgtgtcgtc gctgagcagg
    18781 agccgatcgc ccgcctcctc cgcacgccgc cccagcggcg tgcatcgtca acaggctaaa
    18841 acgatcgccc gcctcctccg cacgccgccc cagcggcgtg catcgtcaac aggctaaaga
    18901 tagcccgttt ggttcaccag tcgcaccgac gttgccccgt cggagtagaa ctcggcgatg
    18961 ctcaacgacg ccaggtcaag gtgcagccgg tacaggatcc ccggcccggc gtccaaagcc
    19021 agccgcagga gcatcttgat cggcgtcacg tgcgacacca ccagcaccgt cgcgccttgg
    19081 tgtgcggcga tgatccggtc gcgtaccctc agcacccggt ccagcacctc gtcgaagctt
    19141 tcaccgcccg gtggcgtggt gccggtgtcg cgtaaccagc gacgatgcag ctccgggtcg
    19201 cgttcggccg attcggcgaa cgtcagcccc tcccaggctc cgaagtcggt ctcgatcaaa
    19261 tcgtcgtcga cggacacgtc caggcccagc gccttggcgg ccgcagccgc ggtgtcatag
    19321 gcccgttgca ggggcgaaga gagcaccgcg gcgattccgc cgcgttgcgc cacgtaacgc
    19381 gccgcggcgt ccgcctgctg tcggccgacc tcggtcagcg ccgggttgcc gcgccccgaa
    19441 taacgccgct gcgccgacaa ttcggtctgt ccgtgccgca gcaacagcag cctggtcggt
    19501 gtgccgcgcg cgccggtcca tccgggcccc gtctcggctt gcttcccaac ggatttcgct
    19561 ggctcggcga ccggcggtgc ggaattttcc gccgggccgg cgacatcggc ggccgcgtcc
    19621 atcgcctcat tggccaaccg atccgcatgc gagttgcggg cccgtggaat ccacgcgtaa
    19681 ctgatccggt cgaacctcga cgccagcgtc cgcgcctggg cgtgcagccc tatcaggtcg
    19741 gggtgcttga ccttccaccg cccggacatc tgttcgacca ccagcttgga atccagaaat
    19801 acctcggcct cggtggcacc cagccgcacg gcgtcgtcca aaccggctat caggccccgg
    19861 tattcggcga cgttgttggt cgcgcgcccg atggcctgct tggcctcggc cagcacggcc
    19921 gaacggtctg cggtgcgcac caccgcgccg taccccgccg gtcctggatt cccgcgcgac
    19981 ccgccgtcgg cttcgatgac gactttcact gctggaaacc tttgacccgc aacaggattg
    20041 ctccacattc cgggcatcgc accaattcgt cgtcggccgc agccgagatg cgggacaaat
    20101 cgccgcgacc gatttcgatg cggcaggcac cgcaccgatg tccgagcaat ggcccggcac
    20161 ccggccctcc cccggcgcgt tgccgctcgt aaagggccga caaggcggga tccagtgtgg
    20221 cgctgagcga gtcacgtcgc gatgaatgtt cctgacgggc ctggtcgatc tcggccacgg
    20281 cggcgtccag ggactgctgg gcgctggcca gctcggcctg cagcgtctcg acgcctccct
    20341 gttcggaatt gagttgggcc tgcatctcct cgcggcgctc catcacctcc agcagggaat
    20401 cttccaggct ggtctgccgg cgaagcaggg tgtccagttc gtgctgaaga tctgacagct
    20461 gccgggcgtc cgtcgcgccc gcgtcgagca tcgaccggtc acggtcttcg cgctgccgca
    20521 ctgcctcgat ctccgactcg aagcgcgaca cctgagcgtc cacgtcttcc agcgcgattc
    20581 ggagcacgcc cagtcggtca gtggcggcgt tgtattcggc ccgcgtcctc tcataggcct
    20641 gttgctgcgg cagatggctg gcgcggtgcg cgatacggga cagctcggca tccagcttcg
    20701 acaactcgag cagcgaaagc tgctgcgcta cttctgcttt catgactcat tcctcccgta
    20761 gtcgtggccc ggacgcccca agttccaagg gtcggtgcgg atggtgcaca cccgcacggg
    20821 cagcgccgct ccgaagtgcg accgcagtat gccggccgcc tgttggcacc agggaaattc
    20881 gctggcccag tgcgccacgt cgatcagggc aacctgcgag gcccggcgat gctcgtcggc
    20941 cggatggtgc cgcagatcgg ccgtgacata cgcctgcacg tccgcgccga ccgcggcggc
    21001 cagcagcgag tcgccggcac cgccgcag
//
LOCUS       DASSGQ010000064        35042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_247795,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000064 DASSGQ010000000
VERSION     DASSGQ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 35042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35042
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247795"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VFI55_02750"
     CDS             complement(<1..904)
                     /locus_tag="VFI55_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HET7072986.1"
                     /translation="MSRLPGSCAIVGIGQTEFSKESGRSELQLACEAVSSALDDAGLA
                     PGDVDGMVTFTMDASDEIDVARSVGVGDLSFFSRVPHGGGAAAGTVVQAAMAVATGVA
                     DVVVCYRAFNERSGFRFGGSGRTNAETPPFMAHYAPFGLLTPAAWVALHAQRYMSTYG
                     VTNEDFGRIAVVDRAHAARNPDAWFYERPITLTDHQNSRWIVEPVLRLLDCCQESDGG
                     VALVVTSAQRARDLRQPPAVITAAAQGAAYNGEMMTSYYREDITGLPEMGVVARQLWR
                     DSGLEPPDIQTAFIYDHFTPFVFTQ"
     gene            complement(901..1365)
                     /locus_tag="VFI55_02755"
     CDS             complement(901..1365)
                     /locus_tag="VFI55_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET7072987.1"
                     /translation="MTTAKTRITTLKWADIEVGDEVTPLEIPVTTTLIVAGAIASRDF
                     MPVHHDRDYASKQGSPNLFMNILTTNGYCVRFLTDWAGPEAMVKKLSIRLGVPCFPDD
                     PLRFTGSVTSKTKGRGEAPAEDFVEITFQGANSLGNHVSGTAVLSLLDGAGA"
     gene            complement(1362..1769)
                     /locus_tag="VFI55_02760"
     CDS             complement(1362..1769)
                     /locus_tag="VFI55_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HET7072988.1"
                     /translation="MAPRLAPAIGPDNEFFWKGLREHRLLLQRCTECEALRHPPRPMC
                     PTCRSVSWDAIEASGRGTVYSYVMPLEPKFPFFDYPYIVVLVQLDEGMRLVSNLCGID
                     PADVTVGMPVEVYYKTFDNDLVLHQFRPAEGSR"
     gene            complement(1769..2317)
                     /locus_tag="VFI55_02765"
     CDS             complement(1769..2317)
                     /locus_tag="VFI55_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HET7072989.1"
                     /translation="MTDREAEFRTKLRALVGQTTGGTGKPTVAPDPVNQPMIRHWAHA
                     LDDMNPVYLDPEFAASSRFGGIVSPPVMLQTWTMPSPMLEGIGERGGAPVEIEGNPTA
                     FLDEAGYTSTVATNSEFEIERYPRLGDVVSATSVFEDVSGEKKTALGTGYFLTWVITY
                     RDQNGEVLGRQRFRVLRFRPEH"
     gene            complement(2314..3525)
                     /locus_tag="VFI55_02770"
     CDS             complement(2314..3525)
                     /locus_tag="VFI55_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7072990.1"
                     /translation="MQTAFPLHSPDFYSGDPYPAYRQLRATSPVCWNDVGKFWALLKY
                     EDIRFVSSNPAMFTSTKGITIPEPGMESPVQPGGLIFTDPPRHRRLRTLINSGFTRRR
                     VAVLEPKIREIVRGILNGIEPHSVHEFAEEIAAPLPTRMIAELIGAPPDDWEQFRVWS
                     DAATGTADPEIELEPVVAVGQLFEYFRKLIAARRTDARDDLLSVLVGAQLDGHRLTDE
                     DLLNFAFLLLVAGNETTRNLIALGTCALIDHPDQRRLLVEDPTLIPGAVEEMLRWNSP
                     VVHMARTAMTDVEIRGQLIAEGDTVVMLYGSANRDEDIFGNDSEEFTVTRSPNPHIAF
                     GCGEHSCIGAQLARLEASVMFDELLRRFPDMELVGEVDRMRATMVPGVKRMPVRLGTG
                     REIDRSGGSGW"
     gene            3740..5209
                     /locus_tag="VFI55_02775"
     CDS             3740..5209
                     /locus_tag="VFI55_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7072991.1"
                     /translation="MAPRQPPEPLVDTYQLYIDGRWVEPENGRYDDIDPATEEIIAGA
                     PDASVAQVGQAIAAARRAFDTGPWATMTPGDRAGCLNQLGDALREHADEFFALSQVEW
                     GCVANERVMQIDGAGYMSMHAAQLATKLGDEPVTGVGVGTTLLRHEPLGVVSILTPWN
                     FPHCLNVMKVNNALAAGNTVVLKPSPLTPLAGLALARIIDEHTNIPPGVVNVVTPSGV
                     EAAKLLTTDPRIDMISFTGSSAVGCEVMRAAGDAMKRLLLECGGKSASIVLDDTEVTD
                     DMLEQMMFDCCSLHAGQACILHSRLLLPASLHDDVVDRLVARARAVRVGDPKDPAAQM
                     GPLISAAQRERVQAHVDGAVNDGAKLVTGGGRPAGLDVGFYFEPTILSEVQPDSAIAQ
                     EEVFGPVLTVLRYRDDDDAVAIANNSRYGLSGAVWSGDVDRAVGVARRIRTGQVAVNG
                     VGPGDAPFGGFKLSGFGREGGGMSALHQYMELKAIGIPA"
     gene            5277..6185
                     /locus_tag="VFI55_02780"
     CDS             5277..6185
                     /locus_tag="VFI55_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7072992.1"
                     /translation="MALAIARPKLEGNVAVGKGRQIGFAEFGEAQGRAVFWLHGTPGA
                     RRQIPTEARVYADSHHIRLIGVDRPGIGSSTPHQYENVAAFADDLGAIADTLGIDKMA
                     VVGLSGGGPYTLACAAALPDRVVAVGVLGGVAPTRGPDAISGGAMNLGLRVAPLLKFG
                     GNPLRIGASLLVQAARPIASPALNVYALVSPQADRHLLTRPEFKAMFLDDLLNGSRKQ
                     LAAPFNDVILFARDWGFRLDEVKVPVRWWHGDHDHIIPFSHGEYVVSRLPDAELFHLP
                     EESHLAGLGRGEEILSTLMKIWDEQA"
     gene            6225..7646
                     /locus_tag="VFI55_02785"
     CDS             6225..7646
                     /locus_tag="VFI55_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HET7072993.1"
                     /translation="MLLASLDPSAITTTDIPDAVRIDGAVLSRSDLVGAATSVAERVA
                     GAERVAILATPTASTVLAITGCLIAGVPFVPVPSDVGVAERRHMLTDSRVQAWVGPVP
                     DDREGLSHIPVRLHARSWHRYPEPSPDAPAMFIYTSGTTGLPKGVQLSRRAIAADLDA
                     LAEAWQWTADDVLIHGLPLFHVHGLVLGLLGSLRIGNRFVHTGKPTPAGYAQAGAEAG
                     GTLYFGVPTVWSRVVADESAARALRPARLLVSGSAPLPVPVFDRLAGLTGHRPIERYG
                     ASESLITISTRADGERRAGWVGLPISGVQTKLLDDNGDEVPHDGETVGRLQVRGPMMF
                     DGYLNRPEATAEAFDADGFYRTGDVAVIDGDGMHRIVGRESVDLIKSGGYRVGAGEIE
                     TSLLGHPGVQEAAVVGLPDEDLGQRIVAFVVGSADLTPDELIGYVAQELSIHKRPREV
                     RIVDALPRNAMGKVLKKQLLSDG"
     gene            7915..9234
                     /locus_tag="VFI55_02790"
     CDS             7915..9234
                     /locus_tag="VFI55_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7072994.1"
                     /translation="MTRVATACLVGSAIEFYDFLIYGTAAALVFPAVFFPHLSPAVAT
                     VASMGTFGAAFLSRPVGAAVFGYFGDHLGRRKTLVATLLVMAVSTVTVGLVPSTRAIG
                     AAAPLILVALRLLQGFAVGGYWAGSALLSAEYAPADKRGRYGMFTHLGGGTALVLSSL
                     TFLAVNFTIGEGSTAFLQWGWRLPFLISAGLIVVALFVWFKIDETPLFAEEKGRHQVP
                     KAPLAEVLHLQGREIALAGGSVLVSFGFVFMAGTYLTTYAHTHLGYSRNLILFAGVLG
                     GLASIASVALSATLCDRVGRRRMMLVGWTGCLLWSFAVIPLVNTGNPVLYAVAIVVMY
                     AVGAIGNAPTAAFIPELFSTRYRYTGSALSVNLAGVIGGALPPLIAGTLLATYGSWAI
                     GLMLAALAMVSLVCTYVLPETNGTALRTTQGVHVGHGSAELELPGGR"
     gene            complement(9236..10189)
                     /gene="dapD"
                     /locus_tag="VFI55_02795"
     CDS             complement(9236..10189)
                     /gene="dapD"
                     /locus_tag="VFI55_02795"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HET7072995.1"
                     /translation="MCAVTGAAGIGLATLASDGSILDTWYPAPELTVSGTSTNSRLAI
                     SDVPPELAALIGRDDDRRTETIAVRTVIGTLDDVAADAYDAYLRLHLLSHRLVAPHEL
                     NTGGLFGVLTNVVWTNRGPCSVIGFEAVRTRLRRHGPVTVYGVDKFPRMVDYVVPSGV
                     RIADADRVRLGAHLAPGTTVMHEGFVNYNAGTLGASMVEGRISSGVVVGDGSDIGGGA
                     SIMGTLSGGGSEVISVGKRCLLGANAGLGISLGDDCVVEAGLYVTPGTKVVTAEGTTV
                     KARDLSGANNLLFRRNSVSGAVEVVARDGQGIALNEDLHAN"
     gene            10247..11311
                     /gene="dapE"
                     /locus_tag="VFI55_02800"
     CDS             10247..11311
                     /gene="dapE"
                     /locus_tag="VFI55_02800"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HET7072996.1"
                     /translation="MLDLGGDPIELTAALVDIPSESRDETRLADEVEAALRAQTSGFE
                     IIRNGNAVLARTRRQRPSRVVLAGHLDTVPVAGNLPSHRENGDLYGCGTADMKSGDAV
                     FLHLAATVAEPVHDLTLIFYDCEEIEAAANGLGRIERELPDWLSADVAILGEPTAGYI
                     EAGCQGTLRVVISATGTRAHSARSWLGDNAIHKLGAVLDRLAGYSARTVDIDGCAYRE
                     GLSAVRIDGGVAGNVIPDAASVTVNFRFAPDRSPPDALQHVRDVFDGLDVHLEQTDAA
                     AGALPGLSQPAAQALVEAAGGLVRAKYGWTDVARFAALGIPAVNFGPGDPNLAHRRDE
                     RVPVANITAAVAMLRRYLGG"
     gene            complement(11324..12820)
                     /locus_tag="VFI55_02805"
     CDS             complement(11324..12820)
                     /locus_tag="VFI55_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET7072997.1"
                     /translation="MRIHPHRAAGLVAAGLLIAALTTDASSVIAPPAASAASTGPTLP
                     LGHVGRWMTDTDGRVVVLHGLNQVYKVPPYEPSADGFGDDDAAFLAANGFNAMRVGVI
                     WAAVEPQPLSYDDNYLASIAQTVATLTSHGIVSLLDFHQDLYNEAFQGEGAPAWAVQT
                     GGLPNPQLGFPGNYFANPAEEFAWNAFWRDAPAPDGIGLHDHYTRAWAHVAAFFRGNP
                     GVFGYDVLNEPWPGFVWEGCFDPVLGCPLQDHKLTEFYRKVVPAIRAADPTTLVFFEP
                     NVLFDEGIHTDVGKVVDPSTGFSFHDYCAIESVLHRNVTCRLEDGVTLTNASLYADIY
                     RIPPLLTEFGATDDLKNLAEVMRHADRHRMGWLEWAYTGNDKTSSSPTAQALVFDPSR
                     PPTGSNVNTAKLAVLAAPYPQVVAGTPKSWSFSSGTFRLCYSTERPDRQGRFAPGAQT
                     LVSVPPIEYPNGYQVSVTGGQVVSAPNAPVLTVVANAGVDTVDVVVTP"
     gene            complement(12954..15182)
                     /locus_tag="VFI55_02810"
     CDS             complement(12954..15182)
                     /locus_tag="VFI55_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7072998.1"
                     /translation="MPIRWNVPQHAAALEQLDAALGPDVSAAPPGEMPAPSPGHQRPA
                     SAPAGAVVLGPDGVGKSTLARLAAEHFVARHPTTHTRWVTGTPTERVVPFGAFSHLVD
                     FSSFGADVGKPAALLRAARASLSRDDRERDLLLVVDDAHDLDVLSATLVYQLALAGTA
                     RMIVTARGPEFGPAPEAIAALWTDDLLDRIDIGPPGGTTKPSEPADVDAFIAELPAAA
                     RSVLDYLAVEEPLSVADLTALAGDGAVAEAADWGAAETRVRGEPANTPVVYTAHPLFS
                     ERARAALGNDGARRRRTELVTLLSRHPSDHLSDRLRLASLALDSDAPQPVAEVVDAAQ
                     QALRVGDPVLAERLARSALERSGDLAARLPLANALAFQGRGRDAEALLAAVDPAALSE
                     PELMSWTLLRAANQFFMLSEPERATAFLQTIRNRVPDAGARITLDALSATFAMNAGNV
                     GRAVEISREVLASASADDQAVAWAASAAALCSARQGRFGDVEPLAQRALGAEHPGLLR
                     FTIGLGQTTTLLMAGRLDAARDLAQQFTDFAELQQPGRAIGEVLLAHVLIAQGEFGPA
                     ARLLGPAAATLERTGYSWGPLSLMLLATALAQQGDIPESAKALRRAETRHGTKSALFA
                     PELRLARAWTKAVARDATGAIAEAREAARMAERAGQSAVAVRAWHDAVRLGDVRAVEP
                     VGRLAAEIDCPVGNIVANHARAWAEGDAAALVAVSEELAAVGMHAAAADAAAQAQRCQ
                     TG"
     gene            15244..15807
                     /locus_tag="VFI55_02815"
     CDS             15244..15807
                     /locus_tag="VFI55_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET7072999.1"
                     /translation="MRPDSDSSRKWAICVYCASGPTDPELLELATELGEAIAERGWTL
                     VWGGGRVSAMGAVASAARARGGWTLGVIPQNLMRREVADTDADELIVSDTMRERKQVM
                     EDRADAFIVLPGGVGTLDELFDAWTTGYLGVHGKPIVMLDPFGHYEGLWLWLGGLLDK
                     GYITQAAMDRLVLVDKVGAAVEACAPA"
     gene            15871..17655
                     /gene="fadD6"
                     /locus_tag="VFI55_02820"
     CDS             15871..17655
                     /gene="fadD6"
                     /locus_tag="VFI55_02820"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase FadD6"
                     /protein_id="HET7073000.1"
                     /translation="MSDHDGGARTAVRLTDILTRAPALLADLPVIVRGLLTGLPVRPS
                     SKKSIGTVFQERAARYGHRIFLKFGDQHLTYRDANAAANRYAAVLAARGVGSGDVVAV
                     MLRNSPNAVLTMLAAVKCGAVAGMLNYHQRGEVLAHSLGLLNAKVLIAETDLVSAVAE
                     CGASGGTETLTIEDVERFAVSAPASNPASASAVQARDTAFYIFTSGTTGFPKASVMTH
                     LRWLKAMAMFGGVGLRLKSSDTLYCCLPLYHNNALTVALSSVLNSGATLALGKSFSAS
                     RFWDEVIASKATAFIYIGEICRYLLNQPPKPTDRAHRVRVIAGNGLRPEIWEEFTKRF
                     GIARVCEFYASSEGNTAFINVFNVPRTTGVFPLPLAYVEYDPDTGTPLRDENGRVRRV
                     PPGQSGLLLSPVNRLQPFDGYTDEVSSEKKLVRNAFREGDCWFNSGDVMSPQGMGHAA
                     FVDRLGDTFRWKGENVATTQVEAALASDHSVEECTVFGVQVPNTGGRAGMAAVKLRDG
                     AEFDGKSLARAVYSHLPAYALPLFVRVVKSLEHTTTFKSRKVALRDQAYGAGVRDPLY
                     VLAGRAEGYVPYYDEYPDEVASGERPQG"
     gene            complement(17724..18570)
                     /locus_tag="VFI55_02825"
                     /pseudo
     CDS             complement(17724..18570)
                     /locus_tag="VFI55_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171618.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18777..19601
                     /gene="folP"
                     /locus_tag="VFI55_02830"
     CDS             18777..19601
                     /gene="folP"
                     /locus_tag="VFI55_02830"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET7073001.1"
                     /translation="MAIVNRTPDSFYDRGATFGDAAARDAAHRAIAEGADVIDVGGVK
                     AGPGETVDSATEIERLVPFIEWLRGAYPDQLISVDTWRSGVARVACAAGADLINDTWG
                     GIDPAMPEVAAEFGAGLVCSHTGGALPRTRPFRVSYGTTPRGVVDDVIRQVTAAAERA
                     VACGVARDRVLIDPAHDFGKNTFHGLALLRHVGDLVMTGWPVLMALSNKDFVGETLGV
                     ELTERLEGTLAATALAAAAGARMFRVHEVAATRRVLEMVSSIQGIRPPARTVRGLA"
     gene            19598..20596
                     /locus_tag="VFI55_02835"
     CDS             19598..20596
                     /locus_tag="VFI55_02835"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HET7073002.1"
                     /translation="MTASELVAGDLTSDEALAARPGDIWLSDRSLNRPTWTIGQLVEA
                     KAGRTVSVVLPALNEEATIESVIDSISPLVRASGGLVDELIVLDSGSTDDTEIRAIAA
                     GARVVSREQALPEVPIRRGKGEALWRSLAATSGDIVVFVDSDLINPHPMFVPWLVAPL
                     LTCDGIHLVKSFYRRPLNVGDVGGGAGATGGGRVTELVARPLLAALRPELGCMLQPLG
                     GEYAATRELLTSLPFAPGYGVEIGLLVDTLDRLGLDAIAQVNLGVREHRNRPLAELGA
                     MSRQVIATLLSRCGIPDSGVGLTQFFAGGPDGFGYTAQTSPVSLADRPPMKGLRPR"
     gene            20636..21001
                     /locus_tag="VFI55_02840"
     CDS             20636..21001
                     /locus_tag="VFI55_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HET7073003.1"
                     /translation="MALVLLYLVVLVLVAVVLFGAASLLFGRGEQLPPLPRGTTATVL
                     PAYGVTGADIDAVKFTQVLRGYKTSEVDWVLDRLARELEALRGQLAAVHASSVVEAAT
                     EGERDPMEPDGGEDRQDTM"
     gene            20998..21576
                     /gene="tag"
                     /locus_tag="VFI55_02845"
     CDS             20998..21576
                     /gene="tag"
                     /locus_tag="VFI55_02845"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET7073004.1"
                     /translation="MNDDGLVRCSWAVTRPGPDGELYRDYHDQEWGRPLHNGVGLFER
                     MSLEAFQSGLSWLIILRKRENFRRAFSGFDIETIAGYADADVQRLLADEGIVRNRAKI
                     EATIANARAVAELGSADDLSQLLWSFAPPPRPRPIDGSEIASATTESKAMARELKRRG
                     FRFVGPTTAYALMQATGMVDDHIRDCWVPSPR"
     gene            21870..22037
                     /locus_tag="VFI55_02850"
     CDS             21870..22037
                     /locus_tag="VFI55_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003945169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3117 domain-containing protein"
                     /protein_id="HET7073005.1"
                     /translation="MAAMKPRTGDGPLEATKEGRGIVMRVPLEGGGRLVVELTPDEAA
                     ALGDELKGVTS"
     gene            complement(22073..23236)
                     /gene="glgA"
                     /locus_tag="VFI55_02855"
     CDS             complement(22073..23236)
                     /gene="glgA"
                     /locus_tag="VFI55_02855"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HET7073006.1"
                     /translation="MRVAMMTREYPPEVYGGAGVHVTELVAQLRRLCAVDVHCMGAPR
                     PDAFAHQPDPRIHGANAALSTLSTDLMMANAASAATVVHSHTWYAGMAGHLTALLYDI
                     PHVLTAHSLEPLRPWKAEQLGGGYRVSTWVEQTAVLAAHAVISVSSAMREDVLRVYPT
                     LDPSLVHVIRNGVDTNVWYPAGPAESGSVLAELGVDPSRPIVAFVGRITRQKGVGHLV
                     AAAHEFNPDVQLVLCAGAPDTPELAEEARAAVAALARSRTGVHWIREMLPIGELREIL
                     SAATVFVCPSVYEPLGIVNLEAMACATAVVASDVGGIPEVVADGITGSLVHYDADDPF
                     GFEAGLAEAVNELVANPDKAKRYGEAGRQRCVEEFSWAHVAEQTLDIYRKVGA"
     gene            23465..24679
                     /gene="glgC"
                     /locus_tag="VFI55_02860"
     CDS             23465..24679
                     /gene="glgC"
                     /locus_tag="VFI55_02860"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET7073007.1"
                     /translation="MREAPHVLGIVLAGGEGKRLYPLTADRAKPAVPFGGAYRLIDFV
                     LSNLVNARYLRICVLTQYKSHSLDRHISQNWRLSGLAGEYITPVPAQQRLGPRWYTGS
                     ADAIYQSLNLIYDEDPDYIVVFGADHVYRMDPEQMVRFHIDTGAGATVAGIRVPRSEA
                     HAFGCIDADESGGIRNFVEKPLEPPGTPDDPDSTFVSMGNYIFTTKVLIDAIRDDADD
                     DHSDHDMGGDIIPRLVGDGMAAVYDFSDNEVPGATERDRGYWRDVGTLDAFYDAHMDL
                     VSVHPVFNLYNKRWPILGASENLAPAKFVNGGSAQESVVGAGSIISAASVRHSVLSSN
                     VVVDDGAIVEGSVIMPGARVGRGAVVRHAILDKNVVVGPGEMVGVDLERDRERFAISA
                     GGVVAVGKGVWI"
     gene            complement(24767..26461)
                     /locus_tag="VFI55_02865"
     CDS             complement(24767..26461)
                     /locus_tag="VFI55_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET7073008.1"
                     /translation="MAKNRAPTLDRPWRRSGALHYALGRIRGIAKPPVTVTDPPAGIV
                     VDRDVSVPTRDGTTLRINVFHASDAGAKPVILSIHPYGKDNLPTRRGKRSTFSVQYRV
                     LRQPVPVTFSTLTGWEAPDPVWWVRHGFTVVNADARGCGHSDGTGKLLSRQEAEDTYD
                     LVQWIAGQPWCDGNVVMLGVSYLAITQYAVAALRPPALRAICPWEGWTDAYRDLTFPG
                     GIRETGFTRLWSRNLRRDTRQTYVLAEMQTDHPLRDEFWRSLAPDLSAIHVPMLVCGS
                     FSDNNLHSRGSIRAFTRGSSSHARLYTHRGGKWVTFYSEAALAEQLKFFRGVLDGAPA
                     SRSVRLEVREDRDTIAAVREESEWPLARTRWRPLYLGGGGMLVTEQPQDSGGITFETL
                     SSAAGFSWTMPADLELTGPMAARLWLELDGCADANLFVGVEKWRDGMFVGFEGSYGYG
                     RDRVTTGWQRVALRALDPELSRPWEPVAACTTPQPIPAGEVVSVDVALGPSATLFRAG
                     EQLRLVVAGRWLCGRNPLTGQFPTAYRSSPRGRVTLHWGPRYDAHLLIPEIPGQPG"
     gene            complement(26489..27163)
                     /locus_tag="VFI55_02870"
     CDS             complement(26489..27163)
                     /locus_tag="VFI55_02870"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015627239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET7073009.1"
                     /translation="MKTGIRVAASSVYGLIVLGLILFLPAGTLDYWQAWVFIVVFTAS
                     TLLPTIYLARANPAALQRRMRAGPRAETRTAQKIIITGSFLGLFATMAFSAFDHRMGW
                     STVPAWVCWLGDVLVAVGLGVAILVVIQNSYAAATVTVETGQKVVSSGVYKYVRHPMY
                     VGNVIMMLGIPLALGSYWGLLFVIPGTMVLVFRILDEEKLLTQELRGYREYTQRVRYR
                     LVPRVW"
     gene            complement(27204..28847)
                     /locus_tag="VFI55_02875"
     CDS             complement(27204..28847)
                     /locus_tag="VFI55_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7073010.1"
                     /translation="MSTATLDRPRHPAHPAPRAGSNFSGTLRILRLDLRRDRVSLPLW
                     VFLLSVPLATVYVGSIEKVYPTQAARAGLAATIMASPAQRALYGQVYNDSVGAVGIWK
                     AGMFHTLIAVAVILTVIRHTRADEETGRTELVDSTAVGRYAGLTAALMLSFGASIATG
                     AIGAAGLLATDIPATGSLAFGVALSGSGLVFSAVAAVAAQLSASARVARGTAFTVLGA
                     AFTLRAVGDAGDGKLSWLSPLGWSLQVRPYAGDRWWVLLLHLATTVALTAVAYRLLAG
                     RDVGAGLIAERPGPGTAAPTLGNVFGLTWRLDRGALLLWTVGLCLYGVVMGSVVHGIG
                     DELGDSSVARDIVARMGGTSSLEEAFIAVAFTLIAMVAAAFAISLTLRLHQEEAGQRA
                     ETILAGAASRSRWVASHLLAALLGSAVAMLVSGAAAGLVYGVAAGDVGGKLAIVVGSA
                     AVQLPAVWLLSAVTVLLFGLAPRFTPVAWGVLVGFVALYLVGSLAGFPQWVLDLEPFS
                     HAPRVGADFTAVPLLWLLAIDAALIIVGIFAFRRRDLRC"
     gene            complement(28847..29767)
                     /locus_tag="VFI55_02880"
     CDS             complement(28847..29767)
                     /locus_tag="VFI55_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7073011.1"
                     /translation="MPTENSAPIEIRGLTKNFGSVRALDGLDLTVREGEVHGFLGPNG
                     AGKSTTIRILLGLVKADGGSARLLGGDPWTDAVELHRQIAYVPGDVTLWPSLTGGETI
                     DLLARMRGGIDQKRRAELIERFDLDPHKKARTYSKGNRQKVSLISAFSSHARLLLLDE
                     PSSGLDPLMENVFQQCVSEARDRGATVLLSSHILAETEVLCERVTIIRAGKTVESGSL
                     DSMRHLSRTSIKAEMTGDPGDLRRIKGVEDLSIEGNTLRAQVDSESLGELIRALGDAG
                     VRSLVSQPPTLEELFLRHYDTGRSESKVSA"
     gene            complement(29757..30398)
                     /locus_tag="VFI55_02885"
     CDS             complement(29757..30398)
                     /locus_tag="VFI55_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET7073012.1"
                     /translation="MRSATDLTAAARIRDAAIEQFGQHGFGVGLRTIADAAGVSAALV
                     IHHFGSKEGLRKACDEFVAEQIRTSKSDALQSSDPATWLGELAEIESYAPLTAYLVRS
                     LQSGGELANMLWNRMIENAESYFEDGVRAGTIKPSRDPRARAKYLAITGGGGLLLYLQ
                     MHENPTDIRAVLRDYARDMVLPALEVYTNGLMADSTMYDAFLAADDQGESHAN"
     gene            complement(30478..31146)
                     /locus_tag="VFI55_02890"
     CDS             complement(30478..31146)
                     /locus_tag="VFI55_02890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET7073013.1"
                     /translation="MDGTDAESPGRAAPSRAESLCAHAEGSISEDAVLVAARERAVEI
                     GAAPVTPAAGALLSVLAKLSGGKAVAEVGTGAGVSGLWLLSGMSNDGVLTTIDIEPEY
                     LRLAKQAFSEAGIGPSRTRLISGRAQDVLTRLADESYDLVFIDADPIDQPDYVVQGVR
                     LLRSGGVIVVHRAALGGRAGDPAARDAEVAAVREAARLIAEDERLTPVLVPLGDGVLA
                     AVRD"
     gene            31370..32158
                     /gene="sigE"
                     /locus_tag="VFI55_02895"
     CDS             31370..32158
                     /gene="sigE"
                     /locus_tag="VFI55_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigE"
                     /protein_id="HET7073014.1"
                     /translation="MGDTVRWNENTGNTGNTKWQLRVDAAERLPNFDGRPNSEDPTIT
                     TLLSPSTMSHAQDRCDDGWAETTDALQGTAVFDATGDKAAMPSWDELVRQHADRVYRL
                     AYRLSGNQHDAEDLTQETFIRVFRSVQNYQPGTFEGWLHRITTNLFLDMVRRRARIRM
                     EALPEDYDRVPADEPNPEQIYHDSRLGPDLQAALDSLPPEFRAAVVLCDIEGLSYEEI
                     GATLGVKLGTVRSRIHRGRQALRDYLAAHPENGARADTSPKASA"
     gene            32320..32724
                     /gene="rseA"
                     /locus_tag="VFI55_02900"
     CDS             32320..32724
                     /gene="rseA"
                     /locus_tag="VFI55_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma E factor RseA"
                     /protein_id="HET7073015.1"
                     /translation="MADRGDVFRRAFSWLPAQFASQSDAPVGAPRQFRSTEHLSVEAI
                     AAFVDGELRMNAHLRAAHHLSLCPECAAEVDDQGRARAALRDSHPIRIPSALLGLLSE
                     IQHYPADDGAASEAPAPDRIADRDARGQRKRR"
     gene            32885..34408
                     /locus_tag="VFI55_02905"
     CDS             32885..34408
                     /locus_tag="VFI55_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET7073016.1"
                     /translation="MTSDHGDDSGNKDSGGGSRLAPRPISRPPVDPASRQLFSRPAGL
                     QGSFVAERVRPQKYRDQAEFKPGEQAADPVLQEAFHRPAGTPSSLQRHPIDAGALAAE
                     KDGAGPDEPADPWRDPAAAAALGTPAVAPSAPQTALGYGGKLGVRDVLFGRKVSYLAL
                     AVLLLIALLIGALGGFIGRKTAEVVEAFTTSKVTLSTYGNGEQPAGRFAKVASATANA
                     VVTIESKSDQEGMQGSGVVIDGRGYIVTNNHVISEAANNPSQFKTTVVFNDGKEVPAN
                     LVGRDPKTDLAVLKVDNVDNLSVAQLGDSDKVRVGDEVLAAGAPLGLRSTVTHGIISA
                     LHRPVPLSGEGSDTDTVIDALQTDASINHGNSGGPLIDMNSQVIGINTAGKSLSDSAS
                     GLGFAIPINEAKEVAQTLIKDGKILHPTLGVSTRSVSNAIAAGAQVANVKAGSPAQKG
                     GILENDVIVKVGNRKVADADEFVVAVRQLTIGQDSPIEVVRDGRHVTLTVKPDPDNG"
     gene            34420..34824
                     /gene="tatB"
                     /locus_tag="VFI55_02910"
     CDS             34420..34824
                     /gene="tatB"
                     /locus_tag="VFI55_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003406260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HET7073017.1"
                     /translation="MFANLSWEHILVLVVVGLVILGPERLPGAIRWTSTTLRQARDYL
                     SGMTSQLRQDLGPEFDELRGPISELQKLRGMTPRAALTKHLLDGDDSFLTGNFDRPVN
                     GAPPNPVEPSGPVARTDEQHAPGPAPFDTDAT"
     gene            complement(34851..>35042)
                     /locus_tag="VFI55_02915"
     CDS             complement(34851..>35042)
                     /locus_tag="VFI55_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HET7073018.1"
                     /translation="DVPLLGQIPLDPALVAAGDAGIPMVLNAPDSPAGKELLSVADSL
                     SSRRRGLAGVSLGVDPTRR"
BASE COUNT         5759 a        11703 c        11900 g         5680 t
ORIGIN      
        1 gctgcgtgaa cacgaacggc gtgaagtggt cgtagatgaa agcggtttgg atgtcgggtg
       61 gctcgaggcc cgaatcgcgc cacaactgcc gggcgaccac tcccatctcc ggcagtccgg
      121 tgatgtcttc gcggtagtaa ctggtcatca tctcgccgtt gtaggcggcg ccctgggcgg
      181 ccgcggtgat caccgccggc ggctgccgta ggtcacgtgc ccgttgcgca ctggtgacga
      241 cgagcgcgac cccgccgtca ctctcctggc agcaatccag cagtcgcagc acgggttcga
      301 cgatccagcg ggagttctgg tggtccgtta gcgtgatcgg gcgctcatag aaccacgcgt
      361 cggggttcct cgccgcgtgt gcgcggtcga cgacggcaat gcggccgaag tcctcgttgg
      421 tcactccgta cgtcgacatg tagcgctggg cgtgcagcgc gacccaggct gcgggagtga
      481 gcaatccgaa cggcgcgtag tgcgccatga acggcggcgt ctctgcgttg gtgcggccgc
      541 tgccgccgaa acgaaagccg gaacgctcgt tgaaggcgcg gtagcacacc accacatcgg
      601 cgacgccggt cgcgacggcc attgccgcct gcaccacggt gcccgccgcg gccccgccgc
      661 cgtgcggcac gcgagaaaag aaactcaggt cgccgacgcc cacgctgcgg gccacgtcga
      721 tctcgtcgct ggcgtccatg gtgaaggtga ccatgccgtc gacatcgcca ggcgccaggc
      781 ccgcgtcgtc gagcgcggaa ctgactgcct cacaggccaa ttgcagctcg ctgcgcccgg
      841 attccttgga gaactcggtc tggccgatcc cgacgatcgc gcagctgccc ggaagccggc
      901 tcatgcgccg gccccgtcca gcaggctgag cacggcggtg ccggagacgt ggttgcccag
      961 actgttggcg ccttggaagg tgatttcgac gaagtcctca gccggcgcct ccccgcgacc
     1021 cttcgtcttg gaggtgacgc tgccggtgaa gcgcagggga tcgtcgggaa agcacggcac
     1081 accaagacga atggacagct tcttgaccat cgcttcgggt ccggcccagt cggtgaggaa
     1141 acgcacgcaa tacccgttgg tggtgaggat gttcatgaac aggttgggcg agccctgttt
     1201 gctggcgtag tcgcggtcgt ggtgcacggg catgaagtcg cgcgacgcga tcgccccggc
     1261 gacgatcagc gttgtggtaa cggggatctc gagtggtgtc acctcgtcgc ccacctcgat
     1321 gtcggcccat ttcaaggttg tgatacgagt cttggcagtc gtcatcggct tccctctgca
     1381 ggccggaact ggtgcagcac gaggtcgtta tcgaaagtct tgtagtagac ctcgaccggc
     1441 atgccaaccg tgacgtcagc cggatcgatg ccacacaggt tggaaaccag ccgcatgccc
     1501 tcgtcgagct gcaccagcac cacgatgtag ggatagtcga agaagggaaa cttcggctct
     1561 agcggcatca cgtagctgta gacggtgccg cggcccgacg cttcgatcgc atcccagctc
     1621 accgaacgac aggtggggca catgggacgg ggcgggtggc gcagcgcttc acattccgtg
     1681 cagcgctgta gcaacagcct gtgctcgcgc agacctttcc aaaagaattc gttgtccggg
     1741 ccgatcgccg gtgccaagcg gggcgccatc agtgctccgg cctgaatcgg agcactcgga
     1801 atcgctggcg ccccagcacc tctccgttct ggtcgcggta ggtgataacc caggtcagaa
     1861 agtagccggt gcccagtgcc gtcttctttt cccctgagac gtcctcgaac accgatgtcg
     1921 cgctgacgac atcgcccagc cggggatagc gttcgatctc gaactccgag ttggtcgcca
     1981 ccgtgctggt gtatccggct tcgtcgagga atgccgttgg gttgccttcg atctcgacgg
     2041 gcgcgccgcc gcgctctccg atcccttcca gcatcggcga cggcatcgtc caggtttgca
     2101 gcatgaccgg cggcgacaca atgccgccga atcgcgacga cgccgcgaat tccgggtcga
     2161 ggtagaccgg gttcatgtcg tcgagcgcgt gcgcccagtg ccggatcatc ggttgattga
     2221 cgggatccgg tgccaccgtt ggcttcccgg tcccgccggt cgtctggccg acgagtgccc
     2281 gcagcttcgt gcggaactcg gcttctcgat cggtcaccaa cccgatcctc ccgatctatc
     2341 tatttcacgg ccagtcccga gccgtaccgg cattcgtttc accccgggca ccatggtggc
     2401 ccgcatccgg tccacctcgc cgaccagctc catgtcggga aaccgccgca gcagctcgtc
     2461 gaacatcacc gaggcctcca ggcgcgccaa ttgcgcaccg atacaagaat gttcaccgca
     2521 tccgaacgcg atgtgggggt tggggctgcg ggtcaccgtg aactcttctg agtcgttgcc
     2581 gaaaatgtcc tcgtcccggt tggccgagcc gtagagcatc accacggtgt cgccttcggc
     2641 gatcagctgc ccgcgtatct cgacgtcggt catcgcggtg cgcgccatgt ggaccaccgg
     2701 gctgttccag cgcaacatct cctcgaccgc gcccggaatc agggtggggt cctcaaccag
     2761 caggcggcgt tgatcgggat ggtcgatcag cgcgcacgtg cccagcgcga tgaggttgcg
     2821 ggtggtctcg ttgccggcga ccaacagcag gaacgcgaag ttgagcaggt cctcgtcggt
     2881 gaggcgatgc ccgtcgagct gcgcgcccac cagaaccgac agcaggtcgt cgcgcgcatc
     2941 ggtgcgcctg gccgcgatca gctttcggaa gtactcgaag agctgtccca cggccaccac
     3001 aggctcgagt tcgatctccg ggtcggccgt tccggtcgcg gcgtccgacc atacccggaa
     3061 ctgctcccag tcgtcgggcg gggcaccgat cagctcggcg atcatccggg taggcagggg
     3121 agcggcgatc tcttcggcga actcgtggac ggaatgcggt tctatcccgt tgaggattcc
     3181 ccgcacgatc tcgcggattt tcggctcgag tacggccacc cgccgccggg tgaaccccga
     3241 gttgatcagc gtgcgcagcc gccggtgtcg cggagggtcg gtgaagatga gcccgcccgg
     3301 ctggaccggg ctctccatgc ccggttcggg gatggtgatg cccttggtcg aggtgaacat
     3361 cgccggattg ctcgacacga aacggatgtc ttcgtacttc agcagcgccc agaacttgcc
     3421 gacgtcgttc caacacaccg gcgaagtggc gcgcagctgc cggtacgccg ggtagggatc
     3481 gcctgagtag aagtccgggg agtgcagcgg gaaagcggtc tgcacgacga acctctttcc
     3541 actgactcgg cgggccagaa tccgacacat tgcatcaaga tgtcctgcga cgttgtgtct
     3601 actctcggcc gaccatctct gtcaatacgc ttgcaacgcg ctgaaatagc gtggaatccg
     3661 cttccaagcg tgattgacgg ccgcaatgca ggggtgtaca ccgagtgatg agatgaccga
     3721 cgttcgggtg aggaagcaga tggccccgcg acaacctccg gaacctcttg tcgacaccta
     3781 tcagctctac atcgacggga gatgggtcga gccggagaac ggtcgctacg acgacatcga
     3841 cccggcgacc gaggagatca tcgccggcgc gcccgacgcc agcgtcgcgc aggtcggtca
     3901 ggcgatcgcg gccgcgcggc gggctttcga cactgggccg tgggcgacca tgacccccgg
     3961 ggaccgggcc ggctgcctga accagctcgg cgacgccctg cgcgagcacg ccgacgagtt
     4021 cttcgcgctg tcacaggtgg agtggggctg cgtcgccaat gaacgcgtga tgcaaatcga
     4081 cggcgccgga tacatgtcca tgcacgcggc ccagctcgcc acgaagttgg gcgacgagcc
     4141 ggtcacgggc gtcggtgtcg gcacgacgtt gctgcgtcac gagccgctgg gcgtcgtttc
     4201 gatcttgacg ccgtggaact tcccgcactg tctcaacgtc atgaaggtga acaacgcgct
     4261 ggccgcgggc aataccgtcg tgctcaagcc ctcgccgctg acgccgctgg ccgggctggc
     4321 gctcgcgcgc atcatcgatg agcacaccaa tatcccaccg ggtgtggtca acgtcgtcac
     4381 gccgtccggt gtcgaggccg ccaagctgct caccaccgat ccgcgcatcg acatgatcag
     4441 cttcaccggc agctcggccg tcggctgtga ggtgatgcgg gcggccggcg acgccatgaa
     4501 gcggctcttg ctggagtgtg gcggaaagtc ggcgagcatc gtgctcgacg acaccgaggt
     4561 gaccgacgac atgctggagc agatgatgtt cgattgctgc tcgctgcacg ccgggcaggc
     4621 ctgcatcctg cacagccgat tactgcttcc cgcttcgttg cacgacgacg tcgtcgaccg
     4681 gctcgtcgcg cgcgcccgag cggtacgcgt cggcgacccc aaagaccctg cggcacagat
     4741 gggtccgctg atcagcgcgg cgcagcggga gcgagtccag gcgcacgtcg acggggcggt
     4801 caacgacgga gcgaagctgg tgaccggcgg tggccgtccg gccggcctgg acgtcggctt
     4861 ctacttcgag ccgacgattc tttctgaggt gcaaccggat tcggccatcg cgcaggagga
     4921 agtcttcggg cccgtgctca cggtgctgcg ctaccgcgac gacgacgacg cggtggcgat
     4981 cgcgaacaac tcgcgctacg ggctgtccgg cgcggtgtgg agcggcgacg tggaccgcgc
     5041 cgttggcgtc gcccgccgca tccgcacggg acaggtggcg gtcaacggcg tcgggcccgg
     5101 cgacgcgccg ttcggcggtt tcaaattgag cgggttcggc cgcgagggcg gcgggatgag
     5161 cgcactgcac cagtacatgg agctgaaggc catcggcatc cccgcatgac gtgatccctt
     5221 ggcggaagcc cggtgagctt ttggtgaacc ctgcgccaca tgcaaagctg ctgggcatgg
     5281 ccctcgcgat tgctcgcccc aagctcgaag gaaacgtcgc cgtcgggaag ggccgccaaa
     5341 tcggctttgc tgaattcggg gaggcgcagg ggcgcgcggt gttctggctg cacggcactc
     5401 ccggcgcccg ccggcagatc ccgaccgagg cccgcgtcta cgccgatagc caccacatcc
     5461 ggctgatcgg cgtcgacaga cccggcatcg gctcgtcgac gccgcatcag tatgagaacg
     5521 tcgccgcatt cgccgacgat ctgggcgcca tcgccgacac gctcggcatc gacaagatgg
     5581 ccgtcgtcgg cctatccggt ggcggtccgt acacccttgc ctgcgcggcg gcgttgcccg
     5641 accgggtggt cgccgtcggc gtgctcggcg gcgtcgctcc cacgcgcggg ccggacgcga
     5701 tcagcggcgg cgcgatgaac ctgggtttgc gggtcgcccc gctgctgaag ttcgggggta
     5761 acccgctgcg catcggcgcc agtctgttag tccaggccgc ccggcccatc gcgtcccccg
     5821 cgctgaacgt ctacgcgctg gtctcaccgc aagccgaccg tcacctgctg acccgccccg
     5881 aattcaaggc gatgtttctc gacgacctgc tcaacggcag ccgcaagcag ctcgccgcac
     5941 cgttcaacga cgtcatcctg ttcgcccggg actggggatt ccggctcgac gaggtgaagg
     6001 tcccggtccg ttggtggcat ggcgaccacg accacatcat cccgttctcg cacggggagt
     6061 acgtcgtctc ccggctgccc gacgccgagc tgttccacct gcccgaagaa agccacctgg
     6121 ccgggctggg ccgtggcgag gagatcctgt ccacgttgat gaagatctgg gacgagcagg
     6181 cctgacccgc agtcgtcggt cggccgccga gtaacctgct ttgcgtgttg ctggcatcgc
     6241 tggacccctc ggccatcacc accaccgaca tcccggacgc ggtcaggatc gacggcgctg
     6301 tgctgagccg cagcgactta gtcggtgcgg cgacgtcggt ggccgaacgc gtcgccggcg
     6361 cggaacgggt tgcgatcctg gccacgccga ccgcgtcgac ggtgctggcg atcaccggct
     6421 gcctgatcgc cggtgtgccc ttcgttccgg tgccctccga cgtcggcgtg gccgagcgtc
     6481 ggcacatgct caccgactcc cgcgtgcagg cctgggtcgg gccggtgcct gacgaccgag
     6541 aagggctgtc gcacatcccg gttcgattgc acgcccgctc gtggcaccgt tatcccgagc
     6601 catcacccga tgccccggcg atgttcatct acacctcggg caccaccggg ctgcccaagg
     6661 gggtgcagct gagccgccgg gcgatcgccg ccgacctcga cgcgctggcg gaggcctggc
     6721 agtggacggc cgacgacgtt ctgatccatg gcctgccgct gttccacgtg cacggactgg
     6781 ttttgggctt gctcgggtcg ctgcggatcg gaaatcggtt cgtgcacacc ggaaaaccga
     6841 caccggcagg ctatgcgcag gctggtgccg aagccggggg cacgttgtat ttcggggttc
     6901 cgacggtgtg gtcgcgggtc gtggccgacg agtcggccgc ccgggcgctg cggccggccc
     6961 ggctgctggt gtccgggagc gccccgctgc cggtgccggt gttcgaccgg ctggccgggc
     7021 tcaccgggca tcggcccatc gagcgatacg gcgcctcgga atcgctgatc accatctcga
     7081 cgcgcgccga cggcgagcga cgcgcgggct gggtgggcct gccgatttcc ggcgtgcaaa
     7141 ccaagctgct ggacgacaac ggcgacgagg tgccacacga cggggaaacc gttgggaggc
     7201 ttcaggttcg cggtccgatg atgttcgacg gctacctgaa tcggcccgag gcgaccgccg
     7261 aagctttcga cgccgacggc ttctaccgca ccggcgacgt cgcggtcatc gacggtgacg
     7321 gcatgcaccg catcgtcggc cgcgagtcgg tcgacttgat caagtccggc ggataccgcg
     7381 tcggcgcggg cgaaatcgaa acgtcgttgc tcgggcaccc cggtgtgcag gaggcggccg
     7441 tggtcgggct gcccgacgag gacctgggcc agcgtatcgt cgcgttcgtc gtcggctcgg
     7501 ccgatctgac gcccgacgaa ttgattgggt atgtcgccca agagctttcg atacacaagc
     7561 ggccgcgtga ggtgcgcatc gtggatgctc tgccgcgcaa tgccatgggc aaggtgctca
     7621 agaagcagct gctctccgac ggctgaaccc cactcgtcag tcgcgagcat gcgcggtggt
     7681 acgcctgatc cggcgtgttg gcgtacgtgg caatccccca gagcgggcaa acccacccgc
     7741 ttgcgggcaa cacgcaggat gttgccagct cgtccatcaa gcgagaccgc gaaccttcac
     7801 ctaccgtcgt aacagtggat agacgcaacc gaccactcgg cgttcttcgt gggtgaggcc
     7861 aatcacccgg gtggctcagc tgcgacggtg gtagcccgcc ctggggccac gccgatgacg
     7921 cgggtggcca cagcctgcct cgtcggctcg gcgatcgagt tttacgactt cctcatctat
     7981 ggcacggcgg cggcgctggt gtttccggcg gtgttcttcc cgcacctcag tcccgcggtg
     8041 gccaccgtcg cttcgatggg gacatttggc gccgcgtttc tgtcgcgccc ggtcggcgcc
     8101 gccgtcttcg gctacttcgg ggaccacctg ggccgaagaa agaccctggt ggccacgctg
     8161 ctggtcatgg cggtgtcaac cgtgaccgtg ggcctggtgc ccagcacgcg agccatcggt
     8221 gcggcagccc cattgatcct ggtcgcgcta cggctgctgc aaggcttcgc ggtcggcggc
     8281 tattgggccg ggtcggcact gctgagcgcc gaatacgcac cggccgacaa acgcgggcgg
     8341 tacggcatgt tcacccacct cggtggcggc accgccttgg tattgagcag cctgaccttt
     8401 ctcgcagtga acttcaccat cggcgagggc agcaccgcgt tcctgcagtg ggggtggcgc
     8461 ctaccgttcc tgatcagcgc ggggctgatc gtcgtcgccc tgttcgtctg gttcaaaatc
     8521 gacgaaactc cattgttcgc cgaggaaaaa ggccgccacc aggtacccaa ggcgcctctc
     8581 gcagaggtac tccacctcca gggccgggaa atcgccctgg ccggcggcag cgtcctcgtt
     8641 tcgtttggtt tcgtgttcat ggccggcact tacctgacca cgtacgccca cacgcacctg
     8701 gggtattccc gcaacctcat cttgttcgcc ggtgtgctgg gcgggctggc gagcattgcg
     8761 tccgtcgcgc tctcggccac tctgtgcgat cgagtcggcc gccgccgcat gatgctggtg
     8821 gggtggacgg gctgcctgct gtggtctttc gcggtgatcc cactggtgaa taccggcaac
     8881 cccgtcttgt atgcggtggc aatcgtggtc atgtacgccg tcggtgcgat cggcaacgca
     8941 ccgaccgcgg cgtttatccc cgagttgttc tccacccgct accgctacac cgggtcggcg
     9001 ctgtcggtga acctcgccgg cgtcatcggt ggcgccctgc cgcccctgat cgccggaacg
     9061 ctgctcgcaa cctacggcag ctgggcgatc gggctcatgc tggcagctct cgcgatggtg
     9121 agcctggtgt gcacctacgt gctgccggaa accaacggaa ccgcgctgcg caccacgcag
     9181 ggtgtgcatg tcggtcacgg gtcggccgag ctggagttgc ccggcggccg gtagttcagt
     9241 tggcgtgcag gtcctcgttg agcgcgatgc cctggccgtc gcgggccacc acctccacgg
     9301 cacccgagac cgagttgcgg cggaacaaca ggttattggc gccggagagg tcgcgggcct
     9361 tgaccgtggt gccctcggcc gtgacgacct tggtgccggg ggtgacgtac aggccggcct
     9421 cgacgacgca gtcgtccccg agcgagatgc ccagcccggc gttggcgccg agcagacacc
     9481 gcttgcccac cgaaatgact tcgctgccac caccggacag cgtgcccatg atggaggctc
     9541 cgccgccgat atccgacccg tcgcccacca ccacaccgga tgagatgcgg ccttccacca
     9601 tcgaggcgcc caaggttccg gcgttgtagt tgacgaagcc ctcgtgcatg acggtggtgc
     9661 ccggcgccag gtgcgcgccc agtcgtaccc ggtcagcgtc ggcgatgcgt accccgctgg
     9721 gcaccacgta gtcgaccatc cgggggaact tgtcgacacc gtagacggtg acgggtccat
     9781 ggcggcgcag ccgtgtccgg accgcctcga aaccgatgac cgagcatggt ccgcgattgg
     9841 tccacacgac gttggtcaag acaccgaaca agccgccggt gttcagctcg tgcggcgcca
     9901 ccagccgatg cgacagcagg tgtagccgca ggtaggcgtc gtaagcgtcg gcggccacat
     9961 cgtccagcgt gccgatgacc gtgcgcacgg cgatcgtctc ggtgcggcgg tcgtcgtcgc
    10021 ggccgatcag cgcggccaac tcggggggga catcggatat cgcaagtcgc gaattcgtgc
    10081 tggtgcccga taccgtcagt tccggtgcgg gataccacgt gtcgaggatc gatccgtcgg
    10141 aggccagcgt cgccagccca atgcctgcag ctccagtcac ggcgcacagg ttacttcccc
    10201 ggccggcggg cggcttgccc ggctcgacaa cggataccct ggggccgtgc tggacttggg
    10261 cggggatccg atcgagttga ccgcggcgct ggtcgacatc cccagcgaat cgagggacga
    10321 gacgcgcctc gccgacgagg tggaggccgc gctgcgtgcc cagacatccg ggttcgagat
    10381 cattcgcaac ggcaatgccg tgctggcgcg tacccggcga cagcggccgt cgcgggtggt
    10441 gctggccgga cacctcgaca ccgtcccggt ggccggaaat ctgcccagtc accgcgagaa
    10501 tggcgatctg tacggctgcg gcaccgcgga catgaaatcc ggtgacgccg tctttcttca
    10561 cctggccgcc accgtggccg aaccggtgca cgacctgacg ctgatcttct acgactgcga
    10621 ggaaatcgaa gcggcggcaa acggtttggg ccgcatcgag cgggagctgc cggactggtt
    10681 gagcgccgac gtggccatcc tgggcgagcc cacggccggc tacatcgagg ccggctgcca
    10741 gggcaccttg cgcgtggtga tcagcgccac cggaactcgc gcccactcgg cgcgttcctg
    10801 gttgggcgac aacgcaattc acaagctggg cgccgtactg gaccgattgg ccggatacag
    10861 cgcacgcacc gtcgacatcg acggctgcgc ctaccgcgag ggcttgtccg cggtgcggat
    10921 cgacggcggt gtggccggca acgtgatccc cgacgccgcc tcggtgacgg tcaacttccg
    10981 tttcgccccc gaccggtcgc cgcctgatgc cctgcaacac gtgcgcgacg tattcgacgg
    11041 cctcgacgtg cacctcgagc agaccgacgc cgcggcgggc gcgctgcccg gtctgtcgca
    11101 gcccgccgcc caggcgctgg tcgaggccgc cggcggactg gtccgcgcga aatacggctg
    11161 gaccgacgtc gcgcggttcg ccgcgctggg cataccggcc gtcaatttcg gccccggcga
    11221 tcccaacctg gcgcaccggc gcgacgaacg ggtgccggtc gccaacatca ccgcggcggt
    11281 ggcgatgctg cggcgctacc tgggcggctg acggctcggc gcttcacggc gtcacgacga
    11341 cgtcgacggt gtcgacgccc gcgttcgcca cgacggtcag caccggggcg ttgggcgccg
    11401 aaacgacctg tccgcctgtg acgctcacct gatagccgtt cggatactcg atggggggca
    11461 ccgagacgag cgtctgcgcg cccggggcga accggccctg gcggtcgggc cgttcggtcg
    11521 agtagcacag ccggaatgtg ccggaactga acgaccagga cttcggtgtg cccgcgacca
    11581 cctgggggta gggcgcggcg agcaccgcga gcttggccgt gttgacgttg ctgccggtcg
    11641 gcggccggct ggggtcgaaa accagcgctt gggccgtcgg cgacgagctc gtcttgtcgt
    11701 tgccggtgta agcccactcc agccagccca tccggtgccg gtcggcgtgg cgcatcacct
    11761 cggcgaggtt cttgaggtcg tcggtggcac cgaactccgt caacagcggc ggaatgcggt
    11821 aaatgtccgc gtacaggctg gcattggtaa gcgtgacccc gtcctccagg cgacaggtga
    11881 cgttgcggtg cagcaccgat tcgatggcgc agtagtcgtg gaaggaaaag ccggtgctcg
    11941 ggtcgaccac cttgccgacg tcggtgtgga ttccctcgtc gaacaacacg ttcggctcga
    12001 agaacaccaa cgtggtcggg tccgcggcac gaatcgcggg cacgactttt cggtaaaact
    12061 cggtcagctt gtggtcctgg agcgggcagc ctaaaaccgg gtcaaagcat ccttcccaca
    12121 caaagcccgg ccatggctcg ttcaacacgt cgtagccgaa cactcccggg ttgccgcgga
    12181 agaaggccgc gacatgtgcc caggcgcgcg tgtagtggtc gtgcagcccg atcccgtccg
    12241 gcgccggggc gtcccgccag aaggcgttcc aggcaaactc ctcggccggg ttggcgaagt
    12301 agttgccggg aaaaccgagt tgcggattgg gcagcccacc cgtttgcacc gcccacgccg
    12361 gcgcgccctc gccctggaac gcctcgttgt agaggtcctg atggaaatcg agcaggctga
    12421 cgatgccgtg cgacgtcaac gtggccacgg tctgcgcgat cgaggcgagg taattgtcgt
    12481 cgtagctgag cggctgcggc tccacggctg cccagatgac gcccacccgc atcgcgttaa
    12541 aaccgttggc agccaggaag gccgcgtcgt catcgccgaa cccgtctgcg gaaggctcgt
    12601 acgggggcac cttgtacacc tggttgagtc cgtgcagcac caccacgcga ccgtcggtgt
    12661 cggtcatcca acggccgacg tggccgagcg gcagcgtcgg ccccgtggac gccgccgacg
    12721 cggccggcgg ggcgatgacc gaggacgcgt cagtcgtcag cgcggcaatc agcaggccgg
    12781 ccgcgaccag gccggcggcg cggtgagggt ggatacgcat ggtgcctcca acccccccgg
    12841 gtcactgcag ctcgcgaaat cactctggaa acagtgatgc gtaatagtca ctctgctaac
    12901 acgggggcgt tacggttcgc gcacaatgcg gcgtgccatt tgcgagctcg ggcctatccg
    12961 gtttggcacc gctgcgcttg tgcagcggca tcggcggccg cagcgtgcat cccgaccgcc
    13021 gccaactcct ccgacaccgc caccagggcg gcggcgtcac cctcggccca tgcgcgggca
    13081 tggttggcga cgatgttgcc caccgggcaa tcgatttcgg cggccagccg gcccaccggc
    13141 tcgacggcac ggacgtcgcc cagccgaacg gcgtcgtgcc aggcccgcac cgccaccgcc
    13201 gactggccgg cacgctccgc catgcgagcg gcctcacggg cctccgcgat tgctcccgtc
    13261 gcgtcccggg cgacggcctt ggtccaggcg cgagccagcc ggagttcggg tgcgaacaac
    13321 gccgacttcg tcccgtgccg ggtctcggcg cgccgcaacg cttttgcgga ttccggtatg
    13381 tcgccctgct gcgccagcgc cgtcgccagc agcatcagcg acagcggtcc ccacgagtag
    13441 cccgtgcgct ccagggttgc ggccgccggg ccgagcagcc tcgccgccgg gccaaattcg
    13501 ccctgcgcga tgagcacgtg cgccaacaac acctcaccga tggcgcggcc cggctgttgg
    13561 agttcggcaa agtcggtgaa ctgctgggcg aggtcgcgcg ccgcatcgag ccgacccgcc
    13621 atcaacagcg tggtggtctg gcccagtccg atggtgaagc gcagcagccc gggatgctcg
    13681 gcgcccaagg cgcgctgcgc cagcggctcg acgtcaccga agcggccctg gcgcgccgag
    13741 cacagtgcgg cggcgctggc cgcccacgcg accgcctggt cgtcggccga cgccgacgcc
    13801 agcacctcgc gggagatctc cacggcccgc ccgacgttgc cggcgttcat cgcgaaggtg
    13861 gcactcagcg cgtcgagagt gattcgcgcg ccggcgtcgg ggacgcggtt gcggatggtc
    13921 tgcagaaacg cggtggcccg ctcgggctcg ctgagcatga agaactgatt ggcggcccgc
    13981 aacagtgtcc aggacatcag ctccggctcg gacaacgcgg cggggtcgac ggctgccagg
    14041 agcgcctccg cgtcgcggcc acgaccttga aacgccaggg cattcgccag cggcagccgc
    14101 gcagccaggt caccggagcg ttccagcgcc gagcgggcca atcgttcggc gagcacgggg
    14161 tcgcccacgc gcagcgcctg ctgtgcggcg tcgacgacct cggcgaccgg ctgcggggcg
    14221 tcgctgtcga gtgccaggga cgccagccgg agccggtcgc tgaggtggtc cgacggatgc
    14281 cgcgacagca acgtgaccag ctcggtgcgc cgccgtcggg cgccgtcatt gccgagcgcg
    14341 gcacgcgctc gttcggagaa cagcgggtgt gccgtgtaca cgacgggggt gttcgcgggt
    14401 tcgccgcgta cccgggtttc ggccgcgccc cagtccgctg cctccgcgac tgcgccgtcg
    14461 ccggcgaggg cggtgagatc cgcgaccgac agcggctcct cgacggccag gtagtccaac
    14521 accgaacgcg ccgcggcagg caactcggcg atgaatgcat cgacgtcagc gggctcggag
    14581 ggcttggtcg taccgccagg cggcccgatg tcgatccggt ccagcagatc gtcggtccac
    14641 agcgccgcta tcgcctcggg cgcgggccca aattcggggc cccgcgcggt gacgatcatc
    14701 cgggccgtac cggccagcgc caactggtac accagggtgg ccgacaggac gtccaggtcg
    14761 tgcgcatcgt cgaccaccag cagcagatcg cgctcccgat cgtcgcggct cagcgatgcc
    14821 cgggccgcgc gtaacaacgc ggccggcttg ccgacgtcgg ccccgaaact ggaaaagtcg
    14881 acgagatggc tgaaggcccc gaacggcacc actcgctcgg tcggggtgcc ggtgacccag
    14941 cgggtgtgtg tggtcgggtg ccgggcgacg aagtgttcgg cggccaagcg ggccagggtg
    15001 gatttgccaa cgccgtcggg tcccagcaca accgcgccgg ccggagccga tgcgggccgc
    15061 tggtggcccg gtgatggtgc gggcatttcg cccggcggtg ccgcggagac gtcagggccc
    15121 agcgcagcgt cgagctgctc caaggccgct gcgtgttggg gtacgttcca tcgaatcggc
    15181 accgaccgga tcatattgcg cgcggttcgc acgccgcacc gggcgtggtc tacgtttcgt
    15241 tgtatgcgcc cggacagcga ttcatcacgc aagtgggcga tttgtgtgta ctgcgcgtcc
    15301 gggccgacgg atcccgaatt gctcgagctc gctaccgaac tcggcgaggc gatcgccgaa
    15361 cgcggctgga cgctggtgtg gggcggcggc cgggtttctg cgatgggtgc ggtggcgagc
    15421 gcggcacgcg cccgcggcgg ttggaccctc ggtgtcattc cgcagaactt gatgcgccgc
    15481 gaggttgccg acacagacgc tgacgagctg atcgtcagtg acaccatgcg cgagcgcaag
    15541 caggtcatgg aggaccgcgc cgatgcgttc atcgtgctgc ccggcggcgt cggcaccttg
    15601 gacgagttgt tcgacgcatg gaccacgggc tatctcggcg tacacggcaa acccatcgtg
    15661 atgctggacc cctttgggca ctacgagggc ctgtggctct ggctgggcgg attgctcgac
    15721 aagggataca tcacgcaagc ggcgatggac cggttggtgc tggtcgataa ggtgggtgcc
    15781 gcggtggagg cgtgcgcacc cgcctgaaag ccggcggccg tacgtcggcg gacaggcgcc
    15841 gaggggagcc gagaagaatt gagggatgac gtgtccgacc acgacggggg agcccgcaca
    15901 gcggtcagat tgaccgatat cctcacccgg gcgccggctc tgttggccga cctgccggtg
    15961 atcgtgcggg gattgctgac cgggctgccg gtccgaccga gctccaagaa atcgatcggc
    16021 acggtgttcc aggaacgggc cgcccgctac ggccaccgga tcttcctgaa attcggcgac
    16081 cagcacctga cctaccgcga cgccaacgcc gcggccaatc ggtacgccgc tgtgctggcc
    16141 gcgcgcggcg ttggcagcgg cgacgtcgtc gccgtgatgc tgcgcaactc gcccaacgcg
    16201 gtgctgacga tgctggccgc ggtcaagtgc ggtgccgtcg ctggcatgct caactaccac
    16261 cagcgcggcg aggtgctggc gcacagcctg ggcctgctca acgccaaggt gttgatcgcc
    16321 gagaccgacc tggtcagcgc cgtcgcagag tgcggcgcct cgggtggcac cgagacgctg
    16381 accatcgaag acgtggagcg attcgccgtg agcgctccgg ccagcaaccc tgcatcggcg
    16441 tcggcggtgc aggccaggga caccgccttc tacatcttca cctcgggcac aacgggattc
    16501 cccaaggcca gcgtgatgac gcatctgcgg tggctcaagg caatggccat gttcggcggc
    16561 gtcgggttgc ggctgaagag ctccgacacg ctgtactgct gcttgccgct gtaccacaac
    16621 aacgccctga cggtggcgtt gtcgtcggtg ctcaattccg gggcgacgct ggcgctgggt
    16681 aagtcgttct cggcgtcgag attctgggac gaggtgattg cgagcaaggc cacggcgttc
    16741 atctacatcg gcgagatctg ccggtacctg ctcaaccaac cgcccaagcc gaccgaccgc
    16801 gcgcaccggg tgcgcgtgat cgccggcaat gggctgcgcc cggagatctg ggaagagttc
    16861 acgaagcggt tcggcatcgc gcgcgtgtgc gagttctacg cctccagcga gggcaacacc
    16921 gctttcatca acgtgttcaa cgtgcccagg accaccggtg tcttcccgtt gccgttggct
    16981 tacgtggaat acgaccccga caccggcacc ccactgcgcg acgagaacgg gcgggtacgc
    17041 cgggtgccgc ccgggcagtc gggcctgttg ctcagcccgg tcaatcggct gcagccgttc
    17101 gatggataca ccgacgaggt gtccagcgaa aagaaattgg tgcgcaacgc ctttcgtgag
    17161 ggggactgct ggttcaactc cggcgacgtg atgagcccac agggcatggg tcacgccgcg
    17221 ttcgtcgacc ggctcggtga caccttccgc tggaagggtg agaacgtcgc caccacccag
    17281 gtcgaagcgg cgctggcgtc cgatcattcc gtcgaggaat gcacggtctt cggcgttcag
    17341 gtgcccaata ccggcggccg cgccgggatg gccgccgtca agctgcgcga cggcgccgag
    17401 ttcgacggca agtccctggc ccgcgcggtg tacagccacc ttcccgccta cgcactgccg
    17461 ttgttcgtgc gagtggtgaa gtccctggag cacaccacga ccttcaaaag ccgcaaggtg
    17521 gcgctgcggg accaggcgta tggcgcgggc gtgcgggatc cgctctatgt gctggccggg
    17581 cgcgccgagg gctatgtgcc gtactacgac gagtaccccg acgaagtcgc ctcgggagag
    17641 cgtccgcagg gctagcggcc ccgtcgaggg ccccgtcgag attgaaacca cgcacactgt
    17701 tgtcggggtt tcgtatgcgt gggttaaacg tcgcgatagc catggcggcg tagcgcttca
    17761 ccggcgcggc ggacgacgtc gtcgggcttg tcctcggcga tcactcggac gatgatccag
    17821 cccagttctt ccagtctgcg cacccgccac tggtcacgca cgtactgtcg acggtcgctg
    17881 cggtgatggt caccgtcgta ttcgacggcc actttgtagt tctcccagcc catatcgagc
    17941 acgccaatgc aacgccagtt ctcctgcacc ggtatctgcg tcgttgggac cggcaaaccc
    18001 gcgttgatca gcagtaaccg cagccacgtc tccttgggag aggcagcgcc ggcatcgacc
    18061 agcgaaagta cggacctcaa ccgccgcagg ccgcgcgcgg gggtgccgct tgacgagcag
    18121 tagcacctct tcgatcgaga aggacttggc acgctttagt gcatcgagtc gcgctactgc
    18181 ctcgccggtg ggcagttgcc gtgccaggtc gaacgccgtc cgcgcgggtg tcgtgacagg
    18241 aagacccgcg acacgggcga gttcgtcacc gtcgagtcgt cggctgcggg tgaccacgcc
    18301 ggcgggtggg tgcgggttac gccagaccat ctcgacgggc tcgtcgtcgt cgatccagct
    18361 cgaaccgtgc aatgccgccg ccgccagccc ggctagcacc ccgcgtcgac ccgaccacag
    18421 ccacgccgca attgagcgtg tgcgcagcga gggcgccgca catttcggca cgtaaacatc
    18481 gggatagatc ggacggaaat cggttcgcag ccgatacggg gttagctttc cctgtcgcag
    18541 ggcttcgctg cctcgaaata cgtcatccac acaccaaaga cgtccccacc agacaacacg
    18601 ctccgcgaaa attaaacgcc gcacaccccg cccgacgtgt cgtgtgcgtg gttgcagtgt
    18661 cgcggcgggt ggtggctggc ctgcccgaaa cggcgcggcc cgcagtcacc aggcaccatg
    18721 gatgcgtgca gtcgactctg tgcggccgac cggtcgcggg ggatcgcccg ctgatcatgg
    18781 cgatcgtcaa ccgcaccccg gattcgttct atgaccgggg tgcaaccttt ggcgacgcgg
    18841 cggccaggga cgcggcgcac cgggccatcg ccgagggcgc cgatgtcatc gacgtcggtg
    18901 gggtcaaagc gggccccggc gaaaccgtcg actcggccac cgagatcgaa cgactggtgc
    18961 cgttcatcga atggctccgc ggcgcctacc cggaccagtt gatcagcgtc gacacctggc
    19021 ggtccggggt ggcccgagtg gcctgcgccg ccggcgcgga cctgatcaac gacacctggg
    19081 gtggcatcga ccccgccatg cccgaggtgg ccgccgagtt cggtgcgggc ctggtgtgtt
    19141 cacacaccgg tggtgcgctg ccgcgcaccc gaccgttccg cgtgagttac ggaaccaccc
    19201 cgcgcggtgt ggtggacgac gtcattcgcc aggtcaccgc cgctgctgag cgggcggtag
    19261 cgtgcggggt ggcccgcgac cgggtgctga tcgacccggc ccacgatttc ggcaagaaca
    19321 cgttccatgg attggctttg ttgcgccacg tcggcgatct tgttatgacc gggtggcctg
    19381 tgctcatggc tttgagtaac aaagacttcg tcggggagac tttgggtgtg gaactgaccg
    19441 aacggcttga gggaacgctg gcggccaccg cgctggcggc cgcggccgga gcgcggatgt
    19501 ttcgggtgca cgaggtcgca gcgacccgcc gggtgctgga gatggtgtcc tcgatccaag
    19561 ggatacgccc gccggcgcgc acggtgaggg ggctcgcatg accgcgtcgg agctggtggc
    19621 cggcgatctc accagcgatg aagccttggc cgctcgcccc ggagacatct ggctgtccga
    19681 ccgcagcctg aaccgtccca cctggaccat cggccaactg gtagaggcga aggccgggcg
    19741 gaccgtctcg gtggtgctgc cggccctcaa cgaggaagcg accatcgaat cggtgatcga
    19801 cagcatctca ccgctggtca gggcctctgg gggtctggtc gacgagctga tcgtgctgga
    19861 ctccggttcg accgacgaca ccgagatccg cgccatcgcc gccggtgccc gcgtcgtcag
    19921 ccgtgaacag gcgttgccgg aggtgcccat ccggcgcggc aagggcgagg cgttgtggcg
    19981 gtcgctggcg gctaccagcg gtgacatcgt ggtgttcgtc gactccgacc tgatcaaccc
    20041 gcacccgatg ttcgtgccgt ggctggtcgc cccgctgctc acctgcgacg gcattcacct
    20101 ggtcaaaagc ttctatcgcc ggcccctcaa tgtcggcgat gtgggtggcg gtgcgggtgc
    20161 caccgggggt gggcgcgtga ccgagctggt ggcccggccg ttgttggcgg cgctgcggcc
    20221 cgagctgggg tgcatgctgc agccgctcgg cggcgaatac gcggccactc gggaactgct
    20281 gacctcgctg ccgttcgccc ccggctacgg cgtggagatc ggcctgctgg tggacacctt
    20341 ggaccggttg ggcctggacg cgatcgccca ggtcaacctc ggggtgcggg agcatcgcaa
    20401 ccggccgctc gccgagctgg gcgcgatgag ccgccaggtc atcgccacgc tgctgtcgcg
    20461 ctgcgggatt cccgactccg gggtcgggtt gacccaattt ttcgccggcg gccccgacgg
    20521 cttcggctac accgcgcaga cgtcaccggt gtcgcttgcg gaccggccgc cgatgaaggg
    20581 gttgcggccg cgctgatccg ccgggttgtc ggcggtatag ggcaatatcg tccacgtggc
    20641 gttggtgttg ctgtacctgg tggtgttggt gctggtcgcg gtggtgctgt tcggcgccgc
    20701 cagcctgctg ttcggtcgtg gtgagcagct gccgccgctg ccccggggga cgacggcaac
    20761 agtccttccg gcctacggtg tcaccggcgc cgatatcgac gccgtcaagt tcacccaggt
    20821 cctgcgggga tacaaaacca gcgaggtgga ctgggtgctg gaccggctcg cccgtgagct
    20881 cgaagcgctg cggggccagc tggccgcggt ccatgcctcg tcggttgtcg aggccgcaac
    20941 cgagggcgaa cgcgatccga tggaaccaga cggcggcgag gatcgtcagg acacaatgtg
    21001 aacgacgacg ggctggtgcg gtgcagctgg gcggttaccc gacccggacc ggacggtgag
    21061 ctgtatcgcg actaccacga ccaggagtgg ggccgcccgc tgcacaacgg ggtggggttg
    21121 ttcgagcgga tgagcctcga ggccttccag agcggcctgt cgtggctgat cattctgcgc
    21181 aagcgggaga attttcgacg cgccttctcg gggttcgaca tcgagacgat cgccggctat
    21241 gcggatgccg atgtgcagcg cctcctggcg gacgagggaa tcgtccgtaa ccgggccaag
    21301 attgaggcga cgatcgccaa cgcgcgtgcc gtcgccgagc tggggtccgc cgacgacctg
    21361 tcccagctgt tgtggtcgtt cgcgccccca ccgcgccccc ggccgatcga cggctcagaa
    21421 atagcttcgg ctacaacgga atccaaggcg atggcacgcg aactcaagcg gcgcggtttc
    21481 cgcttcgtcg gcccgaccac ggcctatgcg ctgatgcagg cgaccggcat ggtcgatgac
    21541 cacattcgcg attgctgggt tccttcaccg cggtgacgat gggggctgcg agcggcccgc
    21601 ggaggaggcg ggcaatccgg ttcagcccgg tgacgatgcg gctctgagcg gcccgtagag
    21661 gaggcgggca atccgattca gcggccccat gacgcaccgg cgaggcgtca caggcgggtc
    21721 tttgtgtaac tgccgactcg aatagggaac aatagagggg tgatttggca gttcaatgtc
    21781 gggtatggct ggaaattcat cggcggggca tgctcggcgc cgaccaggtc cgcgaaggcg
    21841 ggtcagctcg aatctggagg gagcactcga tggcggcgat gaagccccgg accggcgacg
    21901 gtcctctgga agcaaccaag gaggggcgcg gcatcgttat gcgagtacca ctcgagggtg
    21961 gcggtcgact ggtcgtcgag ctgacgcccg acgaagccgc cgccctgggt gacgaactca
    22021 agggcgttac cagctagagc cggctaagcc cgccgaacgg aagctgtcga agtcaggcac
    22081 cgaccttccg atagatgtca agcgtctgct cggcaacgtg ggcccacgaa aattcctcga
    22141 cgcagcgctg gcgtcctgcc tctccgtagc gttttgcttt atccgggttg gcgaccaatt
    22201 cattgaccgc ttcggccaat ccggcttcaa aaccgaacgg gtcgtccgcg tcgtagtgca
    22261 ccagcgaccc ggtgatcccg tcggcgacga cctccggtat tcccccgacg tcggaggcca
    22321 ccaccgccgt agcgcaagcc attgcctcca ggttgacgat gcccaacggt tcgtacaccg
    22381 acgggcagac gaaaacagtt gccgccgaga gtatttcgcg tagttccccg atgggaagca
    22441 tttcccggat ccagtgcacg cccgtccggc tgcgcgccag cgcggctacc gcggcccgag
    22501 cctcttcggc gagctccggg gtgtcgggag caccggcaca caacaccaac tgcacgtcgg
    22561 ggttgaattc gtgcgcggcg gccaccaaat ggccgacgcc cttctgcctg gtgatccgtc
    22621 cgacgaacgc gacgatgggc cggcttggat cgaccccgag ttcggccagc acagacccgc
    22681 tttctgccgg ccctgcggga taccagacgt tggtgtcgac cccgttgcgg atcacgtgca
    22741 ccaggctggg gtccagggtg gggtagaccc gcagcacgtc ttcgcgcatc gcggagctga
    22801 ccgagatgac ggcgtgggcg gcgagcaccg cggtctgctc cacccatgtc gacacccggt
    22861 agccgccgcc gagctgctct gccttccacg gccgcagcgg ctcgagcgaa tgcgccgtca
    22921 ggacatgggg gatgtcgtag agcaacgccg tcagatgccc ggccatgccg gcgtaccagg
    22981 tgtgcgaatg cacgaccgtg gccgccgacg cggcattggc catcatcagg tccgtggaca
    23041 aggtggacag cgccgcgttg gcaccgtgaa tccgtgggtc gggctggtgg gcgaacgcat
    23101 ccggacgggg tgcgcccatg cagtgcacgt cgactgcgca cagccggcgc agctgtgcga
    23161 ccagctcggt gacgtgcacc ccggctccgc catagacctc cggtggatac tcccgcgtca
    23221 tcatcgccac ccgcatacac cgcaccgtag ttcggcgacg ttgccgcccg cgagtcgggc
    23281 gggggaggag ccggacaatt gggttgagtt cggcgacgtt gccgcccgcg agtcgggcgg
    23341 ggcatcggtt gcggcgagcg ggctacccta cggagccaaa tacattgacg gacagcccgt
    23401 cagacagcgg ccaatagcgc gatccccctg gcagcggttc gcggcggccg atagtttgga
    23461 ccccatgagg gaagcgccac acgtgctggg catcgtcctg gccggcggtg agggcaagcg
    23521 gttgtatccg ctgaccgcgg accgggccaa acccgcagtg cccttcggcg gcgcctaccg
    23581 gctgatcgac ttcgtgctct ccaacctcgt caatgctcgc tatttgagga tctgcgttct
    23641 cacccaatac aagtcgcatt cactcgaccg ccatatttcg cagaattggc ggctgtcggg
    23701 tttggccggt gagtacatca cgccggtgcc ggcgcaacag cgtctggggc cgcgctggta
    23761 taccggatcg gcagacgcga tctatcagtc gctcaatctg atctacgacg aagatcccga
    23821 ctacatagtg gttttcggcg ccgatcacgt gtaccggatg gaccccgaac agatggtccg
    23881 gttccacatc gatactgggg ccggcgcaac cgtggccggg atccgggttc cgcgcagcga
    23941 ggctcacgcg ttcggctgca tagacgccga cgaatccggt ggcattcgca atttcgtcga
    24001 gaagccgctg gagccgcccg ggacaccgga cgaccccgac agcacattcg tctcgatggg
    24061 caactacatc ttcaccacca aggtgctcat cgacgcgatc cgcgacgacg ccgacgacga
    24121 ccactcggat cacgacatgg gcggcgacat catccctcgg ctggtgggcg acgggatggc
    24181 cgcggtctac gacttctccg acaacgaggt gcccggcgcg accgagcgcg atcgcggcta
    24241 ctggcgtgac gttgggacgc tggacgcctt ctacgacgcg cacatggacc tggtgtcggt
    24301 gcatccggtg ttcaacctct ataacaagcg ctggccgata cttggcgcgt cggagaacct
    24361 ggcgcccgcg aaattcgtca atggcggctc ggcgcaggag tcggtggtgg gcgccggcag
    24421 catcatctcg gcggcctcgg ttcgccactc cgtcttgtcg tcgaacgtcg tggtcgacga
    24481 cggcgcgatc gtggagggca gcgtgatcat gccgggcgcc cgggtcggcc gcggtgcggt
    24541 ggttcggcac gcgatcctgg acaagaacgt cgtcgtcggt cccggcgaga tggtcggagt
    24601 ggatctggag cgagaccggg aacgcttcgc gatcagcgcc ggcggcgtgg tcgcggtggg
    24661 taaaggtgtc tggatctagc ccggcgacga tgcggcccgg gttgcgggcc gaggtggggc
    24721 gccgccctgc tgcgaagcgg cgacggggag aatcgggcca tcggattcag cccggctgcc
    24781 cgggaatctc cgggatcagc aggtgcgcgt cgtaacgcgg tccccagtgc agtgtgacgc
    24841 ggccgcgcgg cgaactccta taggccgtgg ggaattggcc ggtcagtggg ttgcgcccgc
    24901 acaaccagcg ccccgcgacc accagccgca gttgctcgcc ggcgcggaac agcgtcgcgg
    24961 aggggcccag cgcaacatcg acggagacca cctcgccggc cgggatcggc tgcggcgtgg
    25021 tgcatgcggc gaccggctcc cacggccgcg aaagttcggg atccagtgcg cgtagcgcga
    25081 cccgctgcca gccggtggtg acccggtcgc ggccgtaccc gtacgatccc tcgaatccga
    25141 caaacattcc gtcgcgccac ttctcgacac cgacgaacag gttcgcgtcg gcgcagccgt
    25201 ccagttccag ccacagccga gcagccatcg gcccggtcag ctcgaggtcg gcaggcatcg
    25261 tccagctgaa tcccgctgcg ctggaaaggg tttcgaacgt aataccgccc gagtcttgcg
    25321 gctgttcggt gaccagcatt ccgccgccgc ccagatataa aggccgccaa cgtgtgcggg
    25381 ccagcggcca ctcgctttct tcgcgcaccg cggcgatggt gtcgcgatcc tcgcgcacct
    25441 cgagacgcac gctgcgggat gcgggcgcgc cgtcgagcac gccgcggaag aacttgagct
    25501 gttcggccag cgcggcttcg gaatagaagg tcacccattt gccgccgcga tgtgtgtaga
    25561 gacgagcatg gctggagctg ccgcgggtga aagcccgtat cgaaccgcgg gagtgcaggt
    25621 tgttgtccga gaagctgcca cagaccagca tggggacgtg gatcgccgac aggtctggcg
    25681 ccaaggagcg ccagaattcg tcccgcagtg ggtggtcggt ttgcatctcg gcaaggacgt
    25741 aggtctgccg ggtatcgcgt cgaaggttgc gcgaccacag ccgggtgaag ccggtctccc
    25801 gaattccacc gggaaacgtg aggtcgcggt aagcgtcggt ccagccttcc cacgggcaga
    25861 tcgcccgcag cgccggcggc cgcagggccg cgacggcgta ctgggtgatg gccagatagg
    25921 acacaccgag catgaccaca ttgccgtcgc accacggctg accggcgatc cactgcacca
    25981 ggtcgtaggt gtcctcggct tcctggcgcg acaatagttt tccggtgccg tcggaatggc
    26041 cgcagccgcg cgcgtcggca ttcaccacgg tgaagccatg gcgcacccac cacaccgggt
    26101 ccggcgcctc ccagccggtc agcgtggaga aggtcaccgg tacgggctgg cgtaacacgc
    26161 ggtattgcac ggagaaggtc gagcgcttac ctcgccgggt cggcaggttg tccttgccat
    26221 aggggtggat gctcaagatg accggtttag cgccggcatc gctcgcgtgg aagacgttga
    26281 tccgcaacgt cgttccgtca cgggtaggga cgctgacatc acggtcgacg acgatgcccg
    26341 ccggcggatc agtgacggtt accgggggtt tggcgatgcc gcgaattcgg cccagtgcgt
    26401 agtggagggc accggaacgt cgccagggcc ggtccagagt gggtgcccgg tttttggcca
    26461 catccgccac tgtagagcga gtgggccgtc accacacacg gggaaccaac cgatagcgca
    26521 cccgctgcgt atattcgcgg taaccgcgca attcctgggt gagtagcttt tcctcgtcga
    26581 gaatgcggaa gacgaggacc atagttcccg ggatcacgaa gagaagcccc cagtacgagc
    26641 cgagtgccag gggtatgccc agcatcatga tcacgtttcc gacgtacatc gggtgccgga
    26701 cgtacttgta gacaccgctg gagacgactt tctggcccgt ctccactgtg accgtggcag
    26761 ccgcgtaact gttctgtatg accaccagaa tcgcgacgcc gagcccgacc gccaccagga
    26821 cgtcgccgag ccagcacacc cacgccggca ccgtcgacca gcccatccga tgatcgaaag
    26881 cgctgaacgc catcgtcgcg aagagcccca gaaacgaccc tgtgatgatg atcttttgag
    26941 ctgtcctggt ttccgcgcgc gggccggctc gcatgcgtcg ttgcagagcc gccgggttcg
    27001 ccctggccag atagatcgtg gggagaagcg tcgacgcggt gaatacgacg atgaaaaccc
    27061 atgcctgcca atagtcgagc gtgccggcgg gtaagaacag gatcaaaccg agcacgataa
    27121 gtccgtaaac cgaggatgcc gccacccgga taccagtctt catgagcccc accatccttc
    27181 cggggttgcg ctggcgcgtg taatcaacac cgcagatcac gccgccgaaa agcgaagatt
    27241 cccacaatga tcagcgccgc gtcgatggcc agcagccaca gcagcggcac ggcggtgaag
    27301 tcggcgccga ccctcggagc gtgggagaac ggctcgaggt cgagcaccca ctgggggaac
    27361 ccggccaacg aaccgaccag gtacagggcg acgaacccga ccagcacgcc ccacgcgacg
    27421 ggcgtgaaac gcggtgcgag gccgaacaat aagactgtca ccgcggacag cagccagact
    27481 gcgggcagct gcaccgcggc ggatccgacg acgatggcca gcttgccgcc cacgtcgccg
    27541 gccgcgacac cgtagacgag gccggcggcc gcaccgctca ccagcatcgc gactgccgat
    27601 cccagtagag cagccagcag atggcttgcc acccaacgac ttcgcgacgc cgcgccggcc
    27661 aatatcgtct cggcccgctg gccggcttcc tcctgatgca accgcagggt gagcgagatc
    27721 gcgaaggcgg cggccaccat ggcgatcagt gtgaacgcca ccgcgatgaa cgcctcctcc
    27781 aacgagctgg tgccgcccat ccgcgcgacg atgtcgcggg caacactgct gtcgcccagc
    27841 tcgtcgccga tgccgtgcac cacgctgccc atcaccactc cgtacaggca caggcccacc
    27901 gtccacagca gcagcgcgcc gcggtccagc cgccacgtca gcccgaagac gttgcccaga
    27961 gtcggcgcgg cggtgccggg accgggacgt tcggcgatga gtccggcgcc gacgtcgcgg
    28021 ccggcgagca gtcggtaggc caccgcggta agggccaccg tcgtcgcaag gtgcagcagc
    28081 aggacccacc agcggtcgcc cgcataaggc ctgacctgca gcgaccaccc cagcggcgag
    28141 agccaggaca gcttgccatc accggcgtca ccgacggcgc gcagcgtgaa cgcggctccc
    28201 agcacagtga acgcggtacc ccgggctacc ctggcgctcg ccgacagctg cgcggccacc
    28261 gcggcaaccg cggaaaagac caggccggag ccgctcagcg ccaccccgaa ggccagcgag
    28321 ccggtggccg ggatgtccgt ggcgagcagt ccggcggcgc cgatcgcgcc ggtggcgatc
    28381 gacgctccga acgacagcat cagcgccgcg gtgaggccgg catagcggcc gacggcggtc
    28441 gagtcgacca attcggtgcg gccggtctcc tcgtcagcgc gggtgtgccg gatcacggtg
    28501 agaatgacag ccaccgcgat cagcgtgtga aacatcccgg ccttccaaat gcccaccgcc
    28561 ccaacgctgt cgttgtagac ctgcccgtag agcgcacgtt gggccgggct ggccatgatg
    28621 gtggccgcca atccggcgcg ggcggcctgg gtcgggtaga ccttctcgat gctgccgacg
    28681 tacacggtgg ccagcggcac cgacagcaga aacacccaca ggggcaacga gactcggtcg
    28741 cggcgcaggt cgagccgcag gatccgcagt gtgccggaga agttggaacc tgcccgcggc
    28801 gccgggtgcg ccgggtggcg gggccggtcc agggttgcgg tgctcatcac gctgaaacct
    28861 tgctctcgct gcgtcccgtg tcatagtggc gcaggaacag ttcctcaagg gtcggtggct
    28921 gactcacgag gctgcgcaca cccgcgtcgc cgagcgcccg gatgagttcc ccgaggcttt
    28981 cgctgtccac ctgggcgcgc agcgtgttgc cctcgatgct gaggtcctcg acccctttga
    29041 ttcgtctgag atcgcctgga tcaccggtca tttcggcctt gatggacgtg cggctgaggt
    29101 gccgcatcga gtccagtgaa ccgctctcga cggtcttgcc ggcgcggatg atggtcaccc
    29161 tttcgcacag cacttccgtt tcggccagga tgtgactgga caacaagacc gtcgcaccgc
    29221 gatcgcgcgc ctcgctgacg cactgctgaa acacgttctc catcaacggg tccaggccac
    29281 tgctcggctc atccaacaga agcagcctgg cgtgggagga gaaggccgaa atcagggaga
    29341 ccttttgccg gttgcccttg gagtaggtgc gagccttttt gtgcgggtcg aggtcgaagc
    29401 gctcgatcag ctcggcgcgg cgtttctggt cgatgccgcc gcgcatacga gcgagcaggt
    29461 cgatggtctc gccaccggtc agtgatggcc acaacgtgac atcgccaggc acgtaagcga
    29521 tctggcggtg caattcgacg gcgtcggtcc atgggtcgcc acccagcaac cgcgcgctcc
    29581 cgccgtcggc tttcaccaga cccagcagga tgcggatggt cgtcgacttg ccggccccgt
    29641 tcggcccgag gaatccgtgc acttctcctt cccgcaccgt gaggtcgaga ccatccagcg
    29701 cccgcactga accgaaattc tttgtcaagc cccggatttc gatgggtgcg gagttttcag
    29761 ttggcatggg actctccttg atcgtcggcg gccaggaatg cgtcgtacat ggtgctgtca
    29821 gccattaatc cgttggtgta gacctcgaga gcgggcaaca ccatgtcgcg ggcgtagtca
    29881 cgcaggactg cccggatatc cgttgggttt tcgtgcatct gcagatacag cagcagcccg
    29941 ccgcccccgg tgatggcgag atacttcgcc ctggcccgcg gatcccggct gggttttatc
    30001 gtgccggcgc gcacaccgtc ttcgaagtac gattcggcgt tctcaatcat cctgttccac
    30061 aacatgttcg ccagctcacc gccgctctgc aggctgcgca ccaggtaggc ggtcagtggc
    30121 gcgtaggact cgatctcggc caactcccca agccaggtcg ccgggtcgct ggactggagc
    30181 gcatcggact tgctggtgcg gatctgttcg gcgacgaact cgtcacatgc tttgcgcagg
    30241 ccttccttgg acccgaagtg atggatgacc agcgccgcgc tcactcccgc ggcatcggcg
    30301 atcgtgcgca acccgacacc gaagccgtgc tggccgaact gctcgatggc cgcatcccgg
    30361 atccgggcgg ccgcggtcag atcggtggct gaacgcatgt tcagaacact aaacgcgcgt
    30421 tcagccgaac gtcaaggaga cacccggtca agattccgta aaggttgcgt tccgcagcta
    30481 gtcgcggacc gcggccagca cgccgtcgcc cagcggcacc agcaccggcg ttagccgctc
    30541 gtcctcggcg atcagccggg ccgcctcccg caccgcggcc acctcggcgt cgcgcgccgc
    30601 gggatccccg gcccgcccgc ccagcgccgc ccggtgcacc acgatgacgc cgccggaacg
    30661 cagcagccgc acgccctgca cgacgtaatc cggctggtcg atcgggtcgg catcgatgaa
    30721 caccaggtca tacgactcgt cggcgagccg ggtgagcacg tcttgggcgc ggccgctgat
    30781 cagccgggta cgcgacgggc cgatcccggc ttcggagaaa gcctgcttgg ccagccgcag
    30841 gtactcgggt tcgatatcga tcgtcgtcag cacgccgtcg ttgctcatgc ccgacaacag
    30901 ccagagtccg ctgacgccag cgccggtgcc cacctcggcg acggctttgc cgccgctgag
    30961 cttggccagc acgctcaaca gcgcgccggc ggcgggtgtc acgggtgcgg cgccgatctc
    31021 cacggctcgc tcgcgggcgg ccaccaggac cgcgtcttcc gatatcgacc cctcggcgtg
    31081 cgcgcagagg gattcggctc gactgggggc cgctcggcct ggggattctg cgtcggtgcc
    31141 gtccatgtcc cgcagcgtat tccaattccc ggcgcggccg ggcaggcgcg cctgagtcgg
    31201 gccgtgccga cacgcccgga gccggtgcgg cgacgaagct cactcgcggt cgggttcgcc
    31261 ctgctcaacg caggtaacga tctgatttct caggcaaacc tcagattgct cctatgccgc
    31321 gcatacgccg atacgcgacg gtgtccctat ggaacgcggt gtgcgctcga tgggggatac
    31381 agtgcgctgg aatgagaaca ccggcaatac cgggaatacg aaatggcagc ttcgtgttga
    31441 cgcggctgag cgactgccaa acttcgacgg caggccaaat tcggaggatc caaccatcac
    31501 cacgcttttg agcccgagca ctatgtctca cgcccaggac cgttgcgacg acggatgggc
    31561 ggaaaccacg gatgcgctgc agggcaccgc ggttttcgac gcgaccggcg ataaggcagc
    31621 gatgccatcg tgggacgagc tggtgcgtca gcacgccgac cgggtgtacc ggctggctta
    31681 tcggctgtcc ggcaaccagc acgacgccga ggaccttacc caggagacct tcattcgggt
    31741 cttccgctcc gtccagaatt accaaccggg aacctttgaa ggttggctgc accgcatcac
    31801 caccaacctg ttcctggaca tggtgcgccg gcgtgcccgc atccggatgg aagcgctgcc
    31861 tgaggattac gaccgggtgc ccgcggacga gccgaatccc gagcagattt accacgattc
    31921 gcggcttggc cctgacctgc aggccgcgct ggattcgctg ccgccggagt ttcgcgccgc
    31981 cgtggtgctc tgtgacatcg aaggcctgtc ctacgaagag atcggcgcca ccctcggggt
    32041 gaagttgggc accgtgcgca gccgcataca ccgcggacgt caggcgttgc gcgactacct
    32101 cgctgcgcac ccggaaaacg gcgcccgcgc agacacatcg ccgaaagcgt cggcctagcc
    32161 gaccaccccg cacaatcgct atagagtcat gccatcggcc tgtgcgcagg cgattgccag
    32221 gattgccgcc aacccggcgg cttttgccag ctacagatgc ggtatcgggc gtgacgtcgc
    32281 gctacattcg agtgtgggcg gcaacggaag gagctggcga tggccgaccg aggagatgtg
    32341 tttcgccgcg cgttctcctg gctgccggca cagttcgcct cccagagcga tgcgccggtc
    32401 ggtgccccgc gtcagttccg ttccaccgag catctgtccg tcgaggcgat cgcggcattc
    32461 gtcgatggcg aactgcgcat gaacgcccac ctgcgcgccg cacaccacct ctcgctgtgc
    32521 ccggaatgcg cggccgaagt cgatgatcag ggccgggcgc gcgcggccct gcgcgactcg
    32581 catcccatcc ggatacccag cgcattgctt gggctgcttt ccgaaattca gcactatccg
    32641 gcggatgacg gggccgcttc cgaggccccc gcgccggacc gcatcgccga ccgcgacgcg
    32701 cgcgggcagc gtaagcgccg gtagcttgac agcgttgtcg ctgcgtgtcg ggggcgcgtg
    32761 tgctcatgcc gactaactcc gaactgtgga tactagggtg gacacggacc gcgccggtgc
    32821 ggtgtatagc gcccggtgag gggccaatcg cgagcgctca agaagaacat ccaaaagggg
    32881 taacgtgacc tccgaccacg gcgacgacag cggcaacaag gactccgggg gcggaagccg
    32941 cctggcgccg cggcccatct ctcggccccc ggtcgacccc gcatcgcgtc agctgttcag
    33001 tcggcccgct gggctgcagg gctcgttcgt ggccgagcgc gtgcgcccgc aaaagtaccg
    33061 cgaccaggcc gaattcaagc ccggcgagca agccgccgac ccggtgctgc aggaggcgtt
    33121 tcaccggccg gccgggacac cgagctcgct gcagcgccac cccatcgatg ccggtgcgct
    33181 ggccgccgaa aaggacggtg ccgggccgga cgagcccgcc gatccctggc gagaccccgc
    33241 ggccgcggcc gccctgggta ccccggccgt cgcgccgtcg gcaccgcaga ccgcgctggg
    33301 ctacggcggc aagctcggcg tccgcgacgt gctgttcgga cgcaaggtgt cctaccttgc
    33361 gctggccgtc ctgctcctga tcgcgctgct catcggggcg ctgggcggct tcatcggtcg
    33421 taagaccgcc gaggtcgtcg aggcgttcac cacatcgaag gtcacgctgt cgacctacgg
    33481 caacggcgag cagcccgcgg gccggttcgc caaggtggcg tccgcgaccg ccaacgcggt
    33541 ggtgaccatc gagtccaaga gcgaccagga gggcatgcag ggctccggcg tggtcatcga
    33601 cggccgcggc tacatcgtca ccaacaacca cgtcatctcc gaggcggcga acaatcccag
    33661 ccagttcaag accaccgtgg tcttcaacga cggcaaggaa gtgcccgcca acctggtggg
    33721 ccgcgacccc aagaccgacc tggccgtgct caaggtcgac aacgtcgaca acctgagcgt
    33781 ggctcaactg ggcgattccg acaaggtccg tgtcggcgac gaggtcctcg ccgccggcgc
    33841 gccactgggg ctgcgcagca ccgtgaccca cggcatcatc agcgcgctgc accgacccgt
    33901 cccgctgtcg ggggagggct ccgacaccga caccgtcatc gacgcgctcc agaccgacgc
    33961 gtcgatcaac cacggcaact ccggcggtcc gctgatcgat atgaattccc aggtgatcgg
    34021 catcaacacc gcgggcaagt cgttgtcgga cagcgcgagc gggctgggct tcgcgatccc
    34081 gatcaacgaa gccaaagagg tcgcccagac cctgatcaaa gacggcaaga tcttgcaccc
    34141 gacgctgggg gtcagcaccc ggtcggtgag caacgcgatc gccgccggcg cccaggtcgc
    34201 caacgtgaag gcgggaagcc ctgcgcagaa gggcgggatc ttggaaaacg acgtgatagt
    34261 caaggtcggc aaccggaagg tcgccgacgc cgacgaattc gtcgtcgccg tgcgacagct
    34321 gaccatcggc caggactccc cgatcgaggt ggtccgcgac gggcggcacg tcacgctgac
    34381 ggtgaaaccg gacccggaca atggctagtt gagggctagg tgttcgcgaa cctgagctgg
    34441 gaacacatcc tggtcctcgt cgtggtcggg ctggtgatcc ttggtccgga acggctccca
    34501 ggtgccatcc ggtggacttc gaccacgttg cggcaggcgc gtgactacct cagtggtatg
    34561 acgagccagc tgcgccagga tctcgggccc gagttcgacg agctgcgcgg tccgatcagt
    34621 gagctgcaga agctgcgagg tatgacgcca cgcgccgcgc tcaccaagca cctcctggat
    34681 ggcgacgact cgttcttgac cgggaacttc gaccggcccg tcaatggggc tccgccgaat
    34741 ccggtggagc cgtcaggccc ggttgcgcgc accgatgagc agcacgctcc cggaccggcg
    34801 ccgttcgaca ccgacgcgac ctgaaccgat tcggccgcgg ggccggctcc tcagcgtcgc
    34861 gtcgggtcaa ctcccaatga cacgcccgcc agtccccgcc gccgcgacga caggctgtcc
    34921 gccacgctga gcagttcctt gcccgccggc gagtccggcg cgttgagcac catcggaatc
    34981 cctgcatcac ccgcggcgac cagcgccggg tccagcggga tctgaccgag cagcggcacg
    35041 tc
//
LOCUS       DASSGQ010000065         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1432514,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000065 DASSGQ010000000
VERSION     DASSGQ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432514"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VFI55_02920"
     CDS             complement(<1..1022)
                     /locus_tag="VFI55_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7073019.1"
                     /translation="MMRWIVGSSLRFRLLVLPVAAALTAIGVAQLRAAPVDVLPEFSP
                     PTVQVQTEALGLSAVEVEQFITVPLEQDLLNGVPWLSTIRSQSVAGLSQVDLVFEPGT
                     DVIKARQVVGERLTQAVALPHVSKAPVMIDPVSSTSRVMVIGLSSPDISLIDMSVLAR
                     WQIKPRLQGLPGVANVAIWGQRERQLQVQVDPKRLAAAGVTLNQVVATTGNALWVSPL
                     TFIEASTPGTGGFIETPTQRLTIQHVLPITTAKDLAQVPVEDVAGVPLRLGDVADVVE
                     DHQPLIGDAVVDDGPGLMLVVEKAPGANTLEVTREVEDALQALRPGLGGIKIDTSVYR
                     PATYIES"
     gene            1653..>2167
                     /locus_tag="VFI55_02925"
     CDS             1653..>2167
                     /locus_tag="VFI55_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073020.1"
                     /translation="MTDLVKVIDGVPARVIWDVDYDGNQVAEAELAFFAQDDGDNVWT
                     LGEYPEEYDNGKFAGAPSTWIQGEAHARGGVLVPGHPELGSARFIQGVSRSIDFLDCA
                     QVFKVRQHTCAPTGCYQSVLVMDENSPLDTDSGHQRKSYASGVGVVRIGAVNDPQAET
                     LELTKIVHLGP"
BASE COUNT          368 a          710 c          736 g          353 t
ORIGIN      
        1 gactcgatgt aggtcgccgg ccgatatacc gaagtgtcga tcttgatgcc gcctaggccg
       61 ggccgcagcg cctggagagc atcctcgacc tcgcgggtga cctccagcgt attggcgccg
      121 ggggccttct cgaccacgag catcaggccc gggccgtcat cgacgaccgc gtcaccgatg
      181 agtggctggt ggtcctcgac gacgtcggcc acgtcgccca ggcgcagcgg cacgcccgcg
      241 acgtcctcca ccggcacctg ggcgaggtcc ttggcggtgg tgatcggcag cacgtgctgg
      301 atggtcaggc gttgggtggg tgtttcgatg aatccgccgg tgcccggggt ggacgcctcg
      361 atgaaggtca gcggggagac ccacagcgcg ttgccggtgg tggccacgac ctggttgagc
      421 gtgacgccgg cggcggcgag ccgcttcggg tcgacctgta cctgcagctg ccgctcgcgc
      481 tggccccaga tcgccacgtt ggcgactccg ggcaggccct gcaggcgggg cttgatctgc
      541 cagcgggcca gcaccgacat gtcgatcagg gagatgtcgg gcgaggagag cccgatcacc
      601 atcacccggc tggtcgacga caccgggtcg atcatgaccg gcgccttgga gacgtgcggg
      661 agcgctaccg cctgggtgag ccgttcgccg accacctggc gggctttgat gacgtcggtc
      721 cccggctcga agaccagatc cacctgggac agcccggcga cggactgcga ccggatggtg
      781 gacaaccacg gcaccccgtt gagcaggtct tgttcgaggg ggacggtgat gaactgctcg
      841 acctccaccg cggacaggcc cagagcttcc gtctgcacct gcacggtcgg cgggctgaac
      901 tcggggagca catcgaccgg cgcggcgcgc aactgggcga cgccgatggc ggtcagcgcc
      961 gcggcgacgg gcagcaccag aagccggaac ctgagactcg atccgacgat ccaacgcatc
     1021 attgccgcga cctcccgcgt tgcgcgacgt ttcggtatgg ctactccgcg ccaatggcgc
     1081 gcacaagccc gtgccgaagc cgctggtcgg gggtgtgcgg aatttgcggc tattggctgt
     1141 ccgattcccg acgcaggccc ggaaaccatg acctggctgt gtgttgaata tgagctgcgt
     1201 cacgccagcg gccgatccgc tacggcaatg tttctcggct gatcggacac atagtgtccc
     1261 tcttgacaac tgcctcgcac cggccacagt ggacctacga ctggccggag gtaacgatgg
     1321 cgcgatggac aatcggcgcg aagccacagc gcatgagctg ggcacttgtc gcgtcaaccg
     1381 tgctgctggc cccactgctc gtcggggcgg attcggcttt cgccgccaac cctacaacga
     1441 gtagtaattc gaaagtccct acaacgagta ggaactcggc gtcgcatgcc ggccattcct
     1501 gcgtcgcgtt caaacgattc gacgcggacg atttcccgca ctcgacgaga atcacgggca
     1561 aatggtttcc actcgtcccc ggtacgcagt ttgtctacaa aggaactgtt gtcgaggacg
     1621 gggcctcggt gtcgcatcgg gtggtattca cggtgactga tttggtcaag gtcatcgacg
     1681 gtgtgccggc ccgggtcatc tgggacgtcg actacgacgg caaccaggtc gcggaggccg
     1741 aactcgcctt cttcgcgcag gacgacggcg acaacgtgtg gaccctcggc gagtaccccg
     1801 aggagtacga caacgggaag ttcgccgggg ccccgagcac ctggatccag ggcgaggcgc
     1861 acgcacgtgg cggcgtgctg gttccgggcc accccgagct cggctcggcc cggttcatcc
     1921 agggcgtgtc ccggtccatc gacttcctgg actgcgccca ggtgttcaag gtgcgccagc
     1981 acacctgcgc cccgaccggc tgctaccaga gcgtcctggt catggacgag aacagcccgc
     2041 tcgacacgga cagcggccac cagcgcaagt cctacgcgtc cggggtcggc gtggtacgca
     2101 tcggcgcggt caacgacccg caagccgaga ccctggagct cacgaagatc gtgcacctcg
     2161 ggccgag
//
LOCUS       DASSGQ010000066         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1007375,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000066 DASSGQ010000000
VERSION     DASSGQ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007375"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..431)
                     /locus_tag="VFI55_02930"
     CDS             complement(36..431)
                     /locus_tag="VFI55_02930"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7073021.1"
                     /translation="MTGWPPWTRSPAAAFPAAATTGPRPPTSPSSHCCSTKPRRRATP
                     ARLSRESATVIYPRGTVAMATNGPNTNGSQFFVVFRDAEIEPTSTVFGSVDEAALATL
                     DKIAKVGVAGNRTRGMPASTVTINSVRLG"
     gene            complement(322..1218)
                     /locus_tag="VFI55_02935"
     CDS             complement(322..1218)
                     /locus_tag="VFI55_02935"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7073022.1"
                     /translation="MPTNEQRRATAKRKLERQLERRAKQARWRRILVIAAAAIGAIAV
                     AVVVVIAIIDVKHEHTSNTAASSTSSSAPETSPTTAPADTGPVPPLPAFKPSAGLGAN
                     CQYPASSDPSAKAVKPPRPGKIPTTPAQVSASMVTNEGNLGLMLANNESPCTVNNFAS
                     LANQGYLNNTKCHRLTTSDTLGVLQCGDPKGDGSGGPGYQFANEYPTDQYPPNDPKAQ
                     QPVLYPRGTMAMANAGPNTNGSQFFLVYKDSQLPPQYTVFGTIQSDGLATLDKIAGGG
                     VSGGGDDGAPATDVTIKSLLLD"
     gene            1323..1985
                     /locus_tag="VFI55_02940"
     CDS             1323..1985
                     /locus_tag="VFI55_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7073023.1"
                     /translation="MLITGFPAGALQCNCYVLAERPGTDAVIVDPGQRAMGPLRRILD
                     ENRLTPAAVLLTHGHIDHMWSAQKVSDTYGCPTYIHPEDRFMLKDPIYGIGPRLAQVF
                     TGAFFREPKQVVELDRDGDKLDLGGVIVNVDHTPGHTRGSVVFRVASDADVVFTGDTL
                     FERSVGRTDLFGGSGRDLLTSIVDKLLVLDDKTVVLPGHGNSTTIGAERRLNPFLEGL
                     SR"
     gene            1982..>2073
                     /locus_tag="VFI55_02945"
     CDS             1982..>2073
                     /locus_tag="VFI55_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073024.1"
                     /translation="MTESVAAPKGVPDSVPPESAQVVAVRDGLLT"
BASE COUNT          290 a          650 c          749 g          384 t
ORIGIN      
        1 atatgggaac gccggagagc cagtcgtggg gccgtctagc ccagccgcac cgagttgatc
       61 gtgacggtgc tcgcgggcat gccccgcgtg cggttgccgg ccacacccac cttggcgatc
      121 ttgtcgaggg ttgccagggc cgcctcgtcg accgagccga acaccgtgga cgtcggctct
      181 atctcggcgt cccggaacac cacgaagaac tggcttccgt tggtgttggg tccgttggtg
      241 gccatcgcca cggtcccgcg cggatagatc acggttgctg attctcgact aagccgggcc
      301 ggtgttgcgc ggcgccgggg cttagtcgag cagcagtgac ttgatggtga cgtcggtggc
      361 cggggccccg tcgtcgccgc cgccggaaac gccgccgccg gcgatcttgt ccagggtggc
      421 cagcccgtca ctctgaatcg tgccgaagac cgtgtattgc ggaggtagct gggagtcctt
      481 gtagaccagg aagaactggc tgccgttggt gttgggtccg gcgttggcca tcgccatggt
      541 gccgcgcgga tacagcaccg gctgctgcgc cttggggtcg ttcggcgggt attggtcggt
      601 cgggtactcg ttggcgaatt ggtagccggg accaccgctg ccgtcgccct tggggtcgcc
      661 acattgcagg acgcccaacg tgtccgaggt ggtcagccga tggcatttgg tgttgttgag
      721 gtacccctgg ttcgccaagc tggcgaaatt gttgacggtg cagggcgatt cgttgttggc
      781 gagcatcagc ccgaggttgc cctcgttggt caccatgctc gcgctcacct gggccggagt
      841 ggtggggatc ttgccgggcc gcggcggctt gaccgccttg gcggacgggt ccgacgacgc
      901 cgggtactgg cagttcgcgc ccagaccggc cgacggcttg aacgccggca gcggcggaac
      961 cggtcccgtg tcggccggcg cggttgtcgg gctggtctcg ggggcggagc tgctggtgga
     1021 cgacgccgcg gtgttgctgg tgtgctcgtg tttgacgtcg atgatcgcga tcaccaccac
     1081 gacggccacg gcgatcgcgc cgatcgcggc ggcggcgatc accaggatcc gccgccatcg
     1141 ggcttgcttg gcgcggcgct ccagctgtcg ttccagcttg cgtttggcgg tggcacgtcg
     1201 ctgttcgttg gtcggcacgg ccggtatgcc tccatggttg taagtggggg cgggttagca
     1261 ggcttaggct aatgcgggcg gcgcgagcgg tgtcaagtgg cccccgtggg aaactgggaa
     1321 tcgtgttgat caccggattc cccgccggcg cgttgcagtg caactgctat gtgctggccg
     1381 aacggcccgg gaccgatgcc gtcatcgtgg atccgggaca gcgtgccatg ggtccgttgc
     1441 ggcgcatcct cgacgaaaac aggctgaccc cggccgcggt gctgctcacc cacggacaca
     1501 tcgaccacat gtggtccgcg cagaaggttt ccgacaccta cggctgcccc acctacatcc
     1561 atcccgagga ccggttcatg ctgaaagacc cgatctacgg catcgggccg cggctggccc
     1621 aggtgttcac gggcgcgttc ttccgcgagc ccaagcaggt ggtggagctg gaccgcgacg
     1681 gcgacaagct cgatctgggg ggtgtgatcg tcaacgtcga ccacaccccg gggcacacgc
     1741 gcggatcggt ggtgttccgg gtcgcatcgg atgccgacgt ggtgttcacc ggtgacacgc
     1801 tgttcgagcg ctcggtgggt cgcaccgacc tgttcggcgg cagcgggcgc gacctgctga
     1861 cgtcgatcgt cgacaagctc ctggtgctcg acgacaagac cgtcgtcctg ccgggacacg
     1921 gcaactccac cactatcggc gcggaacggc gcctcaatcc gttcctcgaa ggcctgagcc
     1981 ggtgacggag tcagtcgcgg cgcccaaggg ggtgccggac tccgtcccgc cggagtcggc
     2041 gcaggtcgtc gcggtgcgcg acggcctgct cac
//
LOCUS       DASSGQ010000067         2179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_96057, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000067 DASSGQ010000000
VERSION     DASSGQ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96057"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VFI55_02950"
     CDS             complement(<1..386)
                     /locus_tag="VFI55_02950"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HET7073025.1"
                     /translation="MKTILAVALLGAGIAAAAQQTINVTGDAEVKVVPDRVLISLGVE
                     VRAKSLVEARRENDRRVRAVLDTAKRGGIQPKDIQTDFVQLGIQYQADGLTPQYYYAR
                     KSIELVLYDLNRMESILGSAVDAGAT"
     gene            complement(438..>2179)
                     /locus_tag="VFI55_02955"
     CDS             complement(438..>2179)
                     /locus_tag="VFI55_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073026.1"
                     /translation="TNNLASAVSVSFGACEAALGTGNSFYNSLWQQAAAQGMSVFVSS
                     GDAGAAGCDNPSSTLPAKSGLGVNGLSSTPYNISVGGTQFDDTANAAAYWNETNDGYK
                     ASARSYIPEIVWNESSYLSGSGNNNLFAGSGGVSQIYPTPVWQSGAGVPPADPGAATQ
                     HHRYLPDVSLAAAGHDGYLVYRQGGLYMVAGTSASSPAFAGLAAILNQHSGGRNGNLN
                     PRFYALAAQTPAAYHDVTAGTIAVPCAGGSSGCGSTVAGSVGVINGFPATPGYDLATG
                     LGSVDAYAMVLNWGAHPATAPVISSLNPNPMTASTAAQTLTINGSNFVAGATVQASYP
                     GGAVTTLTVASATATRITATIMTGAVTRAWSIVVTNPNGQSSFAATLQVTAPNPTPAI
                     AGMSPNPMTGSANPQVLSINGSGFQSGAGLAAKLAYAGGPITTLTGGQIVFVNSGQIL
                     ALVAVGTTPRTWSVTVTNPSGLVSNAASLTVVAPPPPPAITSVTPNPMTRSTASQTLT
                     INGTGFTSGAGLRVLAGYPGFSGVLQGSQITSVSETRITVLINVGNTPRNWTIQVQNP
                     NGDTSNQVALQVQ"
BASE COUNT          351 a          705 c          716 g          407 t
ORIGIN      
        1 tgtgtggcgc cggcatccac cgcggaaccg agaatggatt ccatgcggtt gaggtcgtat
       61 agcaccaact caatggattt gcgggcgtag tagtattgcg gcgttaatcc gtcggcctgg
      121 tactggatgc caagctggac gaagtcggtc tgaatgtcct tgggctggat accacctcgc
      181 ttggctgtgt cgaggaccgc ccggacgcgc cggtcgtttt cgcgccgcgc ctcgacaagc
      241 gatttggcgc gcacctctac tccaagcgag atcagtaccc tgtccggtac tacctttacc
      301 tcagcatcgc cggttacatt gatggtctgt tgcgcagcgg ccgcgattcc ggcgcctagc
      361 agcgccacgg caagaatagt cttcataccg tggtaatgcc acggtcgaat gcattccttt
      421 cgaaattccg ctcagtatta ctgcacctgc agcgcgacct gattcgatgt gtcgccattc
      481 ggattctgca cctggatggt ccagttgcgc ggggtgttgc cgacgttgat cagcaccgtg
      541 atcctggttt ccgagacgct ggtgatctgc gagccttgca gcacgccgga gaaaccagga
      601 tagccggcca gcacgcgcag tcccgcgcct gacgtgaatc ccgttccgtt gatggtcaac
      661 gtctggctgg ccgtcgaccg cgtcatcggg ttgggcgtca ccgacgtgat cgccggcgga
      721 ggaggcggcg cgaccaccgt cagactggcg gcattcgata ccaaaccgct cggattggtc
      781 acggtcacgc tccaggtgcg cggcgtggtg ccgaccgcca ccagcgcgag aatctggccg
      841 ctgttgacaa acacgatctg gccgccggtc agcgtcgtga tcggaccgcc tgcataggcc
      901 agtttagccg caagcccggc tcccgattgg aagccgctgc cgttaatcga cagcacctgc
      961 gggttcgccg agcccgtcat cggattcggg ctcattccgg cgatcgccgg agtcggattg
     1021 ggcgcagtca cctgtagcgt cgccgcaaag gacgattggc cgttcgggtt tgtcaccacg
     1081 atactccagg cgcgcgtcac ggcgccggtc atgatggtcg cggtgatcct ggtggcggtc
     1141 gcactggcca cggtcagagt tgtcaccgcg cctcccggat agctggcctg caccgtggct
     1201 cccgcgacga agtttgatcc gttgatggtc agcgtctgcg cggcggtcga cgccgtcatc
     1261 gggttcgggt tcagcgatga gataaccggc gcggtcgccg ggtgcgcgcc ccagttgagc
     1321 accatcgcgt acgcgtccac ggagccgagc ccggtggcga gatcgtaccc cggagtcgcg
     1381 gggaagccgt tgatcacacc caccgagccg gccaccgtcg acccacagcc ggatgacccg
     1441 cccgcgcagg ggaccgcgat ggtgccggcg gtgacgtcgt ggtaggccgc cggggtttgc
     1501 gccgccagcg cgtagaagcg cgggttcaga ttgccgttgc gcccgccgga gtgctggttc
     1561 aggatagcgg ccagtcccgc gaaggccggc gacgatgccg aagtgcctgc caccatgtac
     1621 aagccgccct gccggtagac caggtagcca tcgtggcccg ccgcggccag cgacacgtcg
     1681 ggcaggtagc gatgatgctg cgtcgccgcg cccgggtcgg ccggcgggac acccgcgccg
     1741 ctctgccaca ccggagtcgg ataaatctgg ctgacgccgc cgctgcccgc gaacaggttg
     1801 ttattgcccg agccgctgag gtacgagctt tcgttccaca cgatttccgg aatgtagctt
     1861 cgcgcggagg ccttgtagcc gtcgttggtc tcgttccagt aggccgccgc gttggccgtg
     1921 tcgtcaaact gcgtgccgcc caccgagatg ttgtacggcg tggagctgag cccgttgacc
     1981 ccgaggccgc tcttggccgg cagcgtcgag ctgggattat cgcacccggc cgcgccggca
     2041 tcgcccgacg atacgaacac cgacatcccc tgcgctgcgg cctgctgcca cagcgaattg
     2101 tagaagctgt tgcccgtgcc cagggcggcc tcgcaggctc caaagctgac gctcaccgcc
     2161 gatgcaagat tgttggtga
//
LOCUS       DASSGQ010000068         3472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_825274,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000068 DASSGQ010000000
VERSION     DASSGQ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825274"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFI55_02960"
     CDS             complement(<1..329)
                     /locus_tag="VFI55_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007172560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HET7073027.1"
                     /translation="MRANLADGRDLLFFALPGHCPAPVEDRRPLPERDPDQSQLRFDR
                     STGQWVIVAARRQDRTYKPPADQCPLCPGPTGLTSEVPAPDYDVVVFENRFPSLSGVG
                     EPITVPAG"
     gene            489..2666
                     /locus_tag="VFI55_02965"
     CDS             489..2666
                     /locus_tag="VFI55_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HET7073028.1"
                     /translation="MATRTTAALGCSLLILYLATRASGQPLTVALLGVVITMVASRSV
                     NEPDPGQQRITMALLPVAAALAITAAALLAPHKVASDVVFVAIVFAAAYVRRFGARGR
                     ALGMVGFMAYFLTLYLRARISELPWMIGAVAVGTACTFVMSTYMLPDRPERVLRATVR
                     ALRARMAIVVDTTAEAVRTGRFDERRRRRMRARTIRLNETALMVQGQVEDRADPAMLW
                     PGVTSEQLAPWLFDAELAIEWVATAGRRAAALASDPPGAISASARGELVGALTQLARA
                     IRTPQQGGLQEAAGVAQQVLDQQPGPSDDPGTTAVRRLALAIINAATATAEVRAIVEG
                     TATRAAGTPEIDASSPPVSNSIGSEVGSAPGEQQEKRLLPTTRQAIQVSVAASLAIVV
                     GELVSPARWYWAVIAAFVIFAGTNSWGETLSKGWQRLVGTVLGVPSGVLVATLFAGNK
                     TASLVAIFVCLFCAFYFMTVTYSLMTFWITTMLALLYGLLGEFSFGVLLLRIEETAIG
                     AVIGVTVAILVLPTNTQTAIRNDTREFLTALSALIEISTATMFGAHEAVSPTEQAREL
                     DRKLQQFRLSAKPLLAGVAGLSGRRSIRRSLRLFTACDHFGRSLAYSSERYQAPDRPH
                     RLDHDLGDAFMSASVQTRSNIDALVALIDDADGVTVNPTTDELDAAEALARRLDGKGQ
                     TSPDTQRFLRAVHALRQIEQAIVTAATNLGARDSLKLPSPAAS"
     gene            2727..>3472
                     /locus_tag="VFI55_02970"
     CDS             2727..>3472
                     /locus_tag="VFI55_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019737670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HET7073029.1"
                     /translation="MVPEVTTAKTPEGALLVSADNFTRAESDRYFGNVVKDGGFGNFT
                     HIRELTPLDKQLVVRSNRDTLYSAGVFDLDAGPVSITLPDPGGRFMSMQVITEDHYVP
                     GVFYGRGEHRLIRDDIGTRYVMVAVRTLVDPNDEADLNEVHALQDAVAVRQDHPGSFE
                     IPDWDPISQKAVRDALLQLAATLPDSKQMFGTKDDTDPVRHLIGSAAAWGGNPEKDAI
                     YFSVLPADNGGATAYRLSVDRVPVDGFWSVT"
BASE COUNT          606 a         1158 c         1118 g          590 t
ORIGIN      
        1 ccggcgggca ccgtgatcgg ctcaccgacg cccgataggc tggggaatcg gttctcgaaa
       61 acgacgacgt cgtagtcggg ggcgggcacc tcgctggtca gacctgttgg gcccggacac
      121 agcgggcact ggtcggcggg cggcttgtag gtgcgatctt ggcgccgcgc cgcgacgatc
      181 acccactgtc ccgttgaccg gtcgaaccgc aattgcgact ggtcggggtc acgctccggc
      241 aacggcctgc gatcttccac cggcgccggg cagtgcccgg gcaacgcgaa gaacaacagg
      301 tcacggccat cggccaggtt tgcccgcata ggcaccctca cgacgtcgaa gactagcttg
      361 gtccgcgcag cgaagttcgg gtaaccatgt ggtgttcggt ggacgagggg ggtcaggaga
      421 taacggttct ctacgaaaga gcccgggact ggtggatcgg ctccgatccc ggactgctgc
      481 ggttacggat ggcgactcgg acgacggccg cgctcggctg ttccctgctg atcctttact
      541 tggcgacgcg ggcatcagga cagccgctga ccgtcgccct gctgggcgtc gtcatcacca
      601 tggtcgcctc acgatccgtc aacgagcctg atcccggcca acagcggatc acgatggcgc
      661 tgctgccggt ggccgccgcc ctggccatca ccgccgccgc actgctcgcg ccgcacaagg
      721 tggccagcga cgtcgttttc gtggcgatcg tgttcgccgc ggcatacgtc cgccgtttcg
      781 gtgcccgggg cagggctctg ggcatggtcg ggttcatggc ctatttcttg actttgtatc
      841 tgcgagcccg catttcggag ttgccctgga tgatcggggc ggtcgcggtg ggaaccgcgt
      901 gcacgttcgt gatgagcacc tacatgctgc ccgatcgacc cgagcgcgtg ctgcgagcca
      961 ccgtacgggc gttgcgggcg cggatggcga tcgtggtcga caccaccgcc gaagcagtac
     1021 gcacgggccg gttcgacgag cggcgccggc gtcgcatgcg cgcacgcacc atccggctca
     1081 acgaaaccgc gctgatggtg caaggtcagg tcgaagacag agccgacccc gccatgctgt
     1141 ggcccggcgt gaccagtgaa caactggccc cttggctctt cgacgctgaa ctggccatcg
     1201 aatgggtcgc caccgccggt cgacgagccg cagccctcgc gtcagacccg cccggcgcca
     1261 tctctgccag cgcccgcggc gagctggtcg gggcgctcac acagctcgca cgggcaattc
     1321 gcacaccgca acagggcggg ctccaagagg ccgccggcgt cgcacaacag gttctggatc
     1381 agcagccggg cccctccgac gaccccggca ccaccgccgt acgccgcctt gcactggcga
     1441 tcatcaatgc ggcgacggcg actgccgagg tccgcgcaat tgtcgagggc accgccaccc
     1501 gagcggccgg cacgccggaa atcgacgcaa gttcaccgcc ggtatccaac agcatcggct
     1561 cggaggtcgg ctcagcccca ggcgagcaac aggagaagcg tctgctgcca acaacccggc
     1621 aagccatcca ggtgtcggtc gcggcttcgt tggccatcgt cgttggcgag ctggtgtcgc
     1681 ctgcccgctg gtattgggcg gtgatcgcgg cgttcgtgat cttcgccgga accaactcgt
     1741 ggggcgaaac cctgagcaag gggtggcaac ggctggtcgg caccgtgctt ggcgtgccaa
     1801 gtggagtgct ggtggccacg ctcttcgccg gcaacaagac cgcgtcgctc gttgcgatat
     1861 tcgtctgctt gttctgcgcc ttctatttca tgacggtcac ctacagtctg atgaccttct
     1921 ggatcaccac catgctggca ctgctgtacg gcttgctcgg cgaattctcc ttcggcgtat
     1981 tgcttttgcg gatcgaagag accgcaattg gtgccgtgat cggcgttacc gtggcaattc
     2041 tggtgctacc gaccaatact cagaccgcca tccgtaacga cacccgagaa ttcttgacgg
     2101 cgttgtccgc gctgattgag atatccaccg caaccatgtt cggcgcgcac gaggcggtca
     2161 gccccaccga acaggcacgt gaactcgacc gaaagctgca gcagtttcgg ctcagcgcca
     2221 aaccactttt ggcaggggtc gcaggcctct ccggccggcg aagcattcga cgtagcttgc
     2281 ggctattcac cgcctgcgat catttcgggc gctctctggc gtatagcagc gagcgctacc
     2341 aagcccccga ccgcccgcac cggctggacc atgatctggg cgatgccttc atgtcggcct
     2401 cagtgcagac gcgaagcaac atcgacgcgt tggtcgcgct catcgacgat gccgacggcg
     2461 tgactgtcaa ccccacgacc gacgaactcg atgcggcaga ggcgttggcc cgacgtctgg
     2521 acggaaaagg ccagacttcg cccgataccc aacggttcct cagggcggtg cacgcccttc
     2581 gccaaatcga gcaggcgatc gtcaccgcgg cgaccaacct cggcgcgcgc gacagcctca
     2641 agctgcccag cccggcggcg agctgaacct cacgggttgc cggtccgccg ctgactcgat
     2701 gccgggccac aaatgacccg cggcgtatgg tcccagaggt gacaacggcc aagacgccgg
     2761 aaggcgccct actggtatca gcggacaatt tcaccagggc cgaaagtgac cggtatttcg
     2821 gcaacgtcgt caaggacggc gggttcggca atttcaccca tatccgagaa ctcacgccgt
     2881 tggacaaaca gctggtggtg cggtcgaacc gcgacacgct gtattcggcg ggtgtgttcg
     2941 acctcgacgc cgggccggtc tccataacgc tgcccgaccc gggcggccgc ttcatgtcga
     3001 tgcaggtgat caccgaagac cactacgtgc ccggcgtgtt ctacggtcgg ggcgaacaca
     3061 ggctcatccg cgacgacatc ggcacacggt atgtcatggt cgccgtccgc accctggtcg
     3121 atcccaacga cgaggccgac ctcaacgagg tgcacgcact gcaggatgcc gtcgcggtgc
     3181 ggcaggacca ccccggcagc ttcgagatac ccgactggga cccgatcagc cagaaggccg
     3241 tacgggacgc gcttcttcaa cttgcggcca cgctgccgga ttcgaagcag atgttcggca
     3301 ccaaggacga caccgacccg gtgcgacacc tgatcggctc ggcggcggca tggggtggca
     3361 atccggagaa agacgccatc tatttcagcg tccttccggc cgacaatggc ggcgccaccg
     3421 cgtatcggct gtccgtggac cgggtcccgg tggatgggtt ctggtcggtc ac
//
LOCUS       DASSGQ010000069         5563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_217541,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000069 DASSGQ010000000
VERSION     DASSGQ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217541"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1305)
                     /gene="alr"
                     /locus_tag="VFI55_02975"
     CDS             complement(139..1305)
                     /gene="alr"
                     /locus_tag="VFI55_02975"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET7073030.1"
                     /translation="MAVTPLSLTPGVLAEAVVDLGAIEHNVRLLREHAGRAQVMAVVK
                     ADGYGHGATQVARASLAAGAAELGVATVDEALALRADGITAPVLAWLHPPGIDFGPAL
                     LADVQIAVSSVRQLDELLDAVRRTGRTATVTVKVDTGLNRNGVPPAQYPSMLAALRQA
                     VAEDAVRPRGLMSHMVHADRPDSPINNVQAQRFTDMVAQARNQGLLFEVVHLANSSTT
                     MSRPDLAFDLVRPGIAVYGLSPVPELGDMGLIPAMTVKCAVALVKSIRAGEGVSYGHT
                     WIADRDTNLALLPIGYADGVFRMLGGRLDVLINGRRRPGVGRICMDQFLVDLGPGPLD
                     VAEGDEAILFGPGTRGEPTAQDWADLLGTIHYEVVTSPRGRITRTYREAETIEP"
     gene            complement(1500..2894)
                     /locus_tag="VFI55_02980"
     CDS             complement(1500..2894)
                     /locus_tag="VFI55_02980"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HET7073031.1"
                     /translation="MARHPSLPSHSIAPAYTGRLFTAPVPALRLPEESMDPDAAHRFI
                     HDELMLDGSSRLNLATFVTTWMDPQAGMLMAETFDKNMIDKDEYPATAAIEQRCVCMV
                     ADLFHADDLRDDDPSSAIGVSTIGSSEAVMLGGLALKWRWRAKVGSGKEDGWQTRTPN
                     LVMGSNVQVVWEKFCRYFDVEPRYLPMEEGRYVITPEQVVDAVDENTIGVVGILGTTY
                     TGELEPIAEICAALDKLAAGGGVDVPVHVDAASGGFVVPFLHPELRWDFRLPRVVSIN
                     VSGHKYGLTYPGVGFVVWRSKEYLPEDLVFHVNYLGGDMPTFTLNFSRPGNQVIGQYY
                     NFLRLGRQGYTQVMHSLSSTARWLGDQLRDADQCELISDGSAIPVVSFRLAKDRGFTE
                     FDLSHELRTFGWQVPAYTMPENATNVSVLRVVVREGLSADLARALHDDARTALASLDK
                     VKPAGHYEAQHFAH"
     gene            complement(2918..4348)
                     /locus_tag="VFI55_02985"
     CDS             complement(2918..4348)
                     /locus_tag="VFI55_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET7073032.1"
                     /translation="MRHYYSVDAIRDAEAPLLASLPDGALMRRAAFGLATEIIGELTA
                     RTGGVAGRRVCAVVGSGDNGGDALWAATFLRRRGAAADAVLLNAERTHREGLAAFRKA
                     GGRIVENVLPATDLVIDGVVGISGSGPLRPAAAEVFAGVRDAGIPVVAVDIPSGVDVA
                     TGAITGPAVRAVLTVTFGGLKPVHALADCGRVELIDIGLSLPDTDVLGFEAADVAARW
                     PVPGPHDDKYSQGVTGVLAGSSTYPGAAVLCTGAAVAAHSGMVRYAGGAHAEVLARWP
                     EVIASPTPAAAGRVQAWVVGPGLGTDDTGAAALWFALETDLPVIVDADALTILAAHPE
                     LLKNRAKRGAPTVLTPHAGEFARLAGHPPGDDRVGACRKLADAFGATVLLKGNVTVIA
                     DPGGPVYLNPAGQSWAATAGSGDVLSGMIGALLASGLPPAEAAAAAAFVHARTAALSA
                     ADPGPGDAPTSSSRMVPHIRAALAAL"
     gene            4483..5409
                     /locus_tag="VFI55_02990"
     CDS             4483..5409
                     /locus_tag="VFI55_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7073033.1"
                     /translation="MARTDNDSWDLATSVGATATGVAVGRALASRGADALINDPFAEP
                     LVRAVGVDFFTRLASGELDPSEVDDGAEFGMARMRDMMAVRTRFFDDFFIEATNAGIR
                     QAVILASGLDARAYRLPWPPGTTVYEVDQPRVIEFKTATLKHLRAAPTADRRTVAIDL
                     RHDWPAALREAGLDPAKPTAWSAEGLLPFLPPDAQDALLDNIGLLSAAGSRLATENLP
                     DASRSVPMMADRMREATDRWRDHGFDVEMTDLWYGGDRNDVLEYLNAHGWQAAATSAM
                     DLFAAQGFTLPAAAGEGEAAFASLGYVTASRT"
BASE COUNT          890 a         1919 c         1915 g          839 t
ORIGIN      
        1 tcggcgtaag gatcgtcgat gacggcggcg cgctgggtca tcgacaggcg ggcggaggct
       61 ccgacgatgg cggcgacggc ggccacgcct gctcctcccg caagccaagc actgcccttc
      121 aggcgcctgc gggttttatt acggctcaat ggtttcggcc tcgcggtagg ttctggtgat
      181 ccgcccacgg gggctggtga ccacctcgta gtggatggtg ccgagcaggt cggcccagtc
      241 ctgtgcggtg ggttcgcctc tggtgccggg cccgaacagg atggcctcgt ctccttcggc
      301 gacatcgagc ggtcccggac ccaggtcgac gaggaactgg tccatgcaga tccgccccac
      361 accgggccgc cgcctgccgt tgatcagcac gtccaggcgc ccgcccagca tacgaaaaac
      421 accgtccgcg tagccaatcg gcaatagggc cagattggtg tcacggtcgg cgatccaggt
      481 gtgtccatat gacacgcctt cgccggcgcg gatcgacttc accagcgcaa cagcgcattt
      541 cacggtcatc gctggtatca atcccatgtc gccgagctcc ggaacggggc tcaggccgta
      601 gaccgcgatg cccggtcgca ccaggtcgaa ggccaggtcg ggacgcgaca tcgtggtcga
      661 cgaattcgcc aggtgcacga cctcgaacag cagcccctgg ttgcgcgcct gcgccaccat
      721 gtcggtgaac cgttgcgcct gaacattatt gatcgggctg tcgggccggt ccgcgtgcac
      781 catgtgcgac atcagcccgc gcggccggac ggcgtcctcg gcgacggcct ggcgcagggc
      841 ggccagcatc gacgggtact gcgccggcgg gacaccgttg cggttgagcc ctgtgtcgac
      901 cttgacggtc accgtcgcgg ttcgaccggt ccggcgcacc gcatccagca actcgtcgag
      961 ctgacgcacc gaggacacgg cgatctgcac gtcggccagc aacgccggac cgaagtcgat
     1021 gccgggcggg tgcagccagg ccagcaccgg tgcggtgatt ccgtcagcgc gcagtgccag
     1081 cgcctcgtcg acggtggcga cacccagttc ggccgctcca gcggccaacg acgcgcgggc
     1141 gacctgggtg gccccgtggc cgtagccgtc ggccttgacg acggccatca cctgcgcacg
     1201 gccggcatgc tcgcgcagca gccgaacgtt gtgctcgatg gcacccaggt ccaccacggc
     1261 ctcggcgagg acgcccggag tcagggatag cggtgtaacg gccaacgaag ttttgctccc
     1321 gtgtgtcggt tcgtgccgac attctcccag aaccgttcgg gcacgggatc gaagaggcac
     1381 acccctagtc caactttgtt ccatgggtcg cggttgccac atcagccgga ggcggggaca
     1441 acgaggaccc ttcccgaagg tgaccgatgt cacggatgag acaagacggc gccggcccct
     1501 cagtgcgcga agtgctgcgc ttcgtagtgc ccggccggct tgaccttgtc cagcgacgcc
     1561 agcgcggtgc gggcgtcgtc gtgcagcgcc cgggccaggt cggcggagag cccttcgcgt
     1621 accacgacgc gcagcacgga gacgttggtg gcgttctcgg gcatggtgta cgcgggcacc
     1681 tgccagccga aggtgcgcag ctcgtgggac aggtcgaact cggtgaatcc ccggtctttg
     1741 gccagccgga agctgaccac cgggatcgcc gaaccgtccg agatcagctc gcactggtcc
     1801 gcgtcgcgca gctggtcacc cagccagcgg gcggtcgacg acagactatg catgacctgc
     1861 gtgtagccct ggcggcccag ccgcaggaag ttgtagtact ggccgatcac ctggttgccc
     1921 gggcgggaga agttcagcgt gaaggtcggc atgtcaccgc cgaggtagtt gacgtggaag
     1981 accaggtcct cgggcaggta ctccttgctg cgccatacga cgaagccgac gccgggatag
     2041 gtcagcccgt atttatgtcc gctgacattg atcgacacca cgcgcggcag ccggaaatcc
     2101 catctcagtt cgggatgcag gaatggcacc acaaagccgc cgctggccgc atcaacgtgc
     2161 accggcacgt ccacaccccc gccggcggcc agtttgtcca gtgcggcaca gatctccgcg
     2221 atcggttcga gttcaccggt gtaggtggtg cccaagatcc ccaccacccc gatggtgttc
     2281 tcgtcgacgg cgtcgacgac ctgctcgggc gtgatgacat agcgcccctc ctccatcggc
     2341 aggtagcggg gttcgacgtc gaaatagcgg cagaacttct cccagaccac ctggacgttg
     2401 gagcccatca ccaggttggg ggtgcgcgtt tgccagccgt cttcttttcc ggagccgacc
     2461 ttggcccgcc agcgccactt aagcgccagc ccgccgagca tgaccgcctc gctggagccg
     2521 atggtggaca ccccgatagc actcgagggg tcgtcgtcgc gcaggtcatc ggcatgaaac
     2581 aggtcggcca ccatgcacac gcatcgctgc tcgatggccg cggtcgccgg atactcgtcc
     2641 ttgtcgatca tgttcttgtc gaacgtctcg gccatcagca tgccggcctg ggggtccatc
     2701 caggtggtca cgaacgtcgc caggttcagc cgcgagctgc cgtccagcat cagctcgtcg
     2761 tggatgaagc ggtgggcggc gtcgggatcc atcgactcct cagggagccg cagcgcgggg
     2821 accggcgcgg tgaacaggcg gccggtgtat gcgggcgcga tcgaatgcga aggcaaagac
     2881 gggtgtcggg ccacgagaaa tcctttcgtt gggggagcta gagggcggcc agggcggccc
     2941 ggatgtgcgg aaccatccgt gacgacgacg tcggcgcatc gccggggcca gggtcggcgg
     3001 ccgacagcgc cgccgtacgc gcgtgcacga acgccgcggc cgcggcggcc tcggccggcg
     3061 gcaaccctga cgccagcagc gcgccgatca tcccggacag cacatcgccg gagccggcgg
     3121 tggccgccca ggactgtccg gcgggattga ggtagaccgg gccgccgggg tcggcgatga
     3181 cggtgacatt gcccttgagc agcacggtgg cgccgaacgc gtcggccagc ttgcggcagg
     3241 cgccgacccg gtcgtcgccg ggcggatgcc cggccagccg ggcgaattca cccgcgtgcg
     3301 gagtcagcac cgtcggggcg ccgcgcttgg cgcgattttt tagcagctcg gggtgggcgg
     3361 ccaggatggt cagcgcgtcg gcgtccacga tcaccggaag atcggtctcc agcgcgaacc
     3421 acaacgccgc agccccggtg tcgtcggtgc ccaggcccgg cccgacgacc caggcctgca
     3481 cccgcccggc cgccgccggg gtgggtgagg cgatgacctc gggccaccgg gcgagcacct
     3541 cggcatgtgc gccaccggcg tagcggacca tgccggagtg ggccgcgacg gcggcaccgg
     3601 tgcacagcac ggccgccccc gggtacgtcg acgagcccgc cagcacaccg gtgacgccct
     3661 gggagtactt gtcgtcgtgg gggccgggca ccggccagcg cgcggccacg tcggcggcct
     3721 cgaaacccag gacatccgtg tccggcaggc tcagcccgat atcgatcagc tcgacgcggc
     3781 cgcaatcggc cagggcgtgc acgggtttga gtccgccgaa ggtgacggtc agcacggcgc
     3841 gcaccgcggg cccggtgatc gccccggtcg ccacgtcgac gccgctgggg atgtcgacgg
     3901 cgaccaccgg gatcccggcg tcacgaacac cggcgaacac ctcggccgcc gccggtcgca
     3961 gcggacccga gccggagatg cccaccactc cgtcgatgac gagatcggtt gcgggcaaaa
     4021 cgttctccac aatgcgaccg ccggctttgc gaaacgcggc cagcccttcg cggtgggtgc
     4081 gctccgcatt gagcagcacc gcgtcggcgg ctgcgccgcg acggcgcaga aaggtcgccg
     4141 cccacagcgc gtcgccaccg ttgtctcccg agccgacgac cgcgcacacc cggcggccgg
     4201 cgactccgcc ggtgcgggcg gtcagctccc cgatgatctc ggtggccagc ccgaacgccg
     4261 cgcgccgcat cagggcaccg tcgggcaggc tggccagcag cggtgcctcg gcgtcgcgga
     4321 tagcgtccac agagtagtaa tgccgcatcc gagtcagcgt tccatgcttg cgtcagctcc
     4381 tctgctcgcg gccgatcctg tccgcgctca ccaacattac gtgtcaccat cgaggcgtac
     4441 agtcgcgctc ggctcgattc aatcggcaca cagggggcgc agatggcacg aaccgataac
     4501 gacagctggg atctggcgac cagcgtgggg gccacggcca ccggcgtcgc tgtggggcgc
     4561 gcgctggcca gccgcggcgc ggacgctctg atcaatgacc cgttcgccga gccgctggtg
     4621 cgcgcggtgg gggtcgactt tttcaccagg ctggccagcg gcgaattgga tccctccgag
     4681 gtcgacgacg gcgccgagtt cggcatggcg cggatgcggg acatgatggc ggtgcgcacc
     4741 cgcttcttcg acgacttctt catcgaggcg acgaacgcgg gaatccgcca ggcggtgatc
     4801 ctggcgtcag gcctcgatgc ccgcgcttac cggttgcctt ggccgcccgg caccaccgtc
     4861 tacgaggtcg accagccgcg ggtcatcgag ttcaagaccg ccacgctgaa gcacctccgc
     4921 gccgcgccga ccgccgaccg ccgcaccgtc gccatcgacc tgcgccacga ctggcccgcc
     4981 gcgctgcgcg aggccgggtt ggaccccgcc aaaccgaccg cgtggagcgc tgaaggcctg
     5041 ctgccattcc tgcctccgga tgcccaggac gcgctgctgg acaacatcgg cttgctcagc
     5101 gcggcgggca gccgattggc gaccgagaac ctgccggacg ccagccgttc ggtcccgatg
     5161 atggccgatc ggatgcgcga ggccaccgat cgttggcgcg accacggctt cgacgtggag
     5221 atgaccgacc tgtggtatgg cggcgaccgc aacgatgtgc tcgagtacct gaacgcccac
     5281 ggctggcagg ccgctgccac cagcgccatg gatctatttg ccgcccaagg ctttacgttg
     5341 ccagcagccg ccggagaagg ggaagcggcg ttcgcctcgc tgggctacgt cactgctagc
     5401 cgaacctagc caggcggtgt cgaatacgct tgccggacga cgactttcgc cgacgccccg
     5461 gtcaggttga tgcctcgtgg gatcggagtc gccgcacggt ctgcttgaac cgcgcgggat
     5521 tccactgcgg cttcttgttc gcccgggcca tcggatagaa gca
//
LOCUS       DASSGQ010000070        21457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_35767, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000070 DASSGQ010000000
VERSION     DASSGQ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 21457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21457
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35767"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VFI55_02995"
     CDS             complement(<1..761)
                     /locus_tag="VFI55_02995"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET7073034.1"
                     /translation="MTDSPLMLAPSPGSEVVRQHFTVTGVVQGVGFRPFVHRIASELG
                     LAGFVGNDSGAVFLEVQGARARVDEFDRRLRHEAPPLARIGAVTVTDLTADTACRREF
                     RIVASQSVAGAHTPKTPIPPDVAVCDDCVAELFDPHNRRYRHPFVTCTNCGPRFTIIR
                     ELPYDRPATTMSAFTMCARCAAEYHDPSDRRFHAQPIACPDCGPSLWFRPAGATQGGE
                     RTQASDAALAATQGALAAGVVVAIKGIGGYHLACAV"
     gene            complement(763..1029)
                     /locus_tag="VFI55_03000"
     CDS             complement(763..1029)
                     /locus_tag="VFI55_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HET7073035.1"
                     /translation="MCLGIPGQVIRMLDGYEGQLALVDVVGEQRRVNVGMLPEVTFSP
                     GDWIIIHMGFAVEKTDRAGAEQAMAGLELMGRGRTDTTVSQGDG"
     gene            complement(1032..1559)
                     /locus_tag="VFI55_03005"
     CDS             complement(1032..1559)
                     /locus_tag="VFI55_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HET7073036.1"
                     /translation="MTARILVAGIGNIFLGDDGFGSEVVRNAEIPQNDPSVRVIDYGI
                     RGMHLAYDLLEEWDTLVLVDAVPSRGNPGMLHVFQADHEEPSAPGRVSGSGATGLDGH
                     SMDPAAVFASLRALGGNPPYTVVIGCEAGSVEEGIGLTEPVAKAVPRAARAVEEIVAA
                     LQTPATLQSPLRQED"
     gene            complement(1689..3048)
                     /locus_tag="VFI55_03010"
                     /pseudo
     CDS             complement(1689..3048)
                     /locus_tag="VFI55_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393662.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3045..3698)
                     /locus_tag="VFI55_03015"
     CDS             complement(3045..3698)
                     /locus_tag="VFI55_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020728304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HET7073037.1"
                     /translation="MTGQQLGVDFAVLDVAPEPYRVSPVLTARVSVATDGEDPVHAIA
                     LRCQVRIEPLRRPYSDAEADGLIDLFGLRERWATTQRTFLWQHCSTMVPGFAGNTTVG
                     LPLDCTYDFEVAAAKYMHALRDGTIPLQFLFSGTIFVKSERGFSVQQVPWDCEYRYDM
                     PVAAWRELITQHYPNTGWVRLSHETIAALAGYKSAQGLLDLDHAVTALLDAERETAQ"
     gene            complement(3695..4342)
                     /locus_tag="VFI55_03020"
     CDS             complement(3695..4342)
                     /locus_tag="VFI55_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5947 family protein"
                     /protein_id="HET7073038.1"
                     /translation="MTGAYDVLARIRSNQRAPGPAGERCEMCSELVADEHQHVVNVTG
                     RQLMCVCRACYLLFTDGGAELRYRAVPDRYLAFPDFALDRRAWDALQIPVGVAFFFTN
                     SALGRTVAFYPGPAGATESELDLDAWNAISGADSRVGLLADDVEALLVRVPADGEAAQ
                     PQSYLVPIDACYEFVGRLRLLWRGFDGGQEARAFIDNFFAQIAARAVTTQAGTPP"
     gene            complement(4339..4410)
                     /locus_tag="VFI55_03025"
                     /pseudo
     CDS             complement(4339..4410)
                     /locus_tag="VFI55_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217793.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
     gene            complement(4416..5354)
                     /locus_tag="VFI55_03030"
     CDS             complement(4416..5354)
                     /locus_tag="VFI55_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HET7073039.1"
                     /translation="MADRPTAPPEGPDSDAKWRTAGDRIQTLLDSCAASGATAYERAQ
                     QLVREVVELYGAGLERVMLLAQAPGQGPALAERLAADDLVASLLLVHGLHPHDVHRRV
                     SDALDRVRPYLGSHGGDVDLLDITGDTVHLAFRGSCKSCPSSAVTLELAVEDAVRAAA
                     PEISAIEVVAAEPATTPAGMIPAESLLARVHSNGPSNGHGATTWHAVPELAELGPGEV
                     AGFAIDGATVLACRVDERLFAYRDRCPVCDDTLAGSQLHGPLLRCRRCQTDFDVVHAG
                     AGVTGGSRDHLDPIPLLTRDGVLSVASRGQPMGAPA"
     gene            complement(5358..7157)
                     /locus_tag="VFI55_03035"
     CDS             complement(5358..7157)
                     /locus_tag="VFI55_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HET7073040.1"
                     /translation="MTTIIPEPSHATREPGQLVEMAWDPITRIVGSLGIYTKIDFENK
                     EVVECHSTSSIFRGYSIFMKGKDPRDAHFITSRICGICGDNHCTCSCYTQNMAYGVKP
                     PHLGEWIVNLGEAAEYMFDHNIFQENLVGVDFCEKMVSETNPSVLSLAEKTPAPHADA
                     HGYKTIADIMRSLNPFSGEFYREALQVSRWTREMFCLMEGRHVHPSTLYPGGVGTVAT
                     IQLMTDYMTRLMRYVEFMKKVVPMHDDLFDFFYEALPGYEKVGLRRTLLGCWGSFQDP
                     EVCNFNYKDMERWGNAMFVTPGVVVDGELVTHSLVDINLGIRILLGSSYYDDWTEQEM
                     FVKTDPLGNPVDRRHPWNQHTNPHPQKRDMDGGKYSWVMSPRWFDGKDHLALDTGGGP
                     LARLWATALAGLVDIGYVKATGNSVKINLPKTVLKGPVELEWKIPQHGSNTIERDRAR
                     TYFQAYAAACALHFAEQALAEIRAGRTKTWEKFEVPDEAIGCGFTEAVRGVLSHHMVI
                     RDGKIANYHPYPPTPWNANPRDSFGTPGPYEDAVQGQPIFEENSRENFKGIDVMRTVR
                     SFDPCLPCGVHMYLGEGKTLDRLHSPTQSPAGE"
     gene            complement(7238..8293)
                     /locus_tag="VFI55_03040"
     CDS             complement(7238..8293)
                     /locus_tag="VFI55_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012691718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypE"
                     /protein_id="HET7073041.1"
                     /translation="MPTEAAVKAEETLIHVLWINAGLSCDGDSVALTAATQPSVEEIA
                     LGALPGLPKVAVHWPLIDFECGPTGGADDFLEWFFKADRGELEPFVLVVEGSIPNEKI
                     KDEGYWCGFGNNPATGQPMTTSEWLDRLTPKATAVVAVGTCATYGGIHAMAGNPTGAM
                     GVPDYLGWDWKSKAGIPIVCVPGCPIHPDNLSETLTYLLYMATGQAPMIPLDEALRPK
                     WLFGNTVHEGCDRAGYYEQGDFATEYGSPKCIVKLGCWGPVVKCNVPKRGWINGIGGC
                     PNVGGICIGCTMPGFPDKFMPFMDEPPGGKFSSTASGLYGTVIRNLRGITARTVDKEP
                     RWRHKGDQLTTGARRTW"
     gene            complement(8612..9388)
                     /gene="hypB"
                     /locus_tag="VFI55_03045"
     CDS             complement(8612..9388)
                     /gene="hypB"
                     /locus_tag="VFI55_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HET7073042.1"
                     /translation="MGRFHRHDDGTVHTHEHDHLPREHDHGDHSGYETGKQRIDVLEA
                     IFAENDLLADANRAAFESNGIRTLNLMSSPGSGKTTILQATLDELSAEFAIGVIEGDI
                     ATDLDAAKLSGRGAQVSLLNTSNGFGGECHLDAPMVNRALPGLDLASLDLVIVENVGN
                     LVCPAEFDVGEHAKAMVYSVTEGEDKPLKYPVMFRSVDVVLLNKIDLVPYLDVDVDSY
                     IAHVREVNATATILPVSARTGAGMGAWFGWLRRFADDADS"
     gene            complement(9417..9752)
                     /locus_tag="VFI55_03050"
     CDS             complement(9417..9752)
                     /locus_tag="VFI55_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HET7073043.1"
                     /translation="MHELSLCEAIAGVVKTHADGRHVDVVRVRIGALRQVVPDSLSFC
                     WTLVRDSEDMPDAELELECVSAEVKCRSCGRQSEITSAWSIWCPLCDSADVEVLRGNE
                     FLVTSLDVS"
     gene            9812..10147
                     /locus_tag="VFI55_03055"
     CDS             9812..10147
                     /locus_tag="VFI55_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073044.1"
                     /translation="MVILNLTGRSVKVDELFASIEAHRPGRDDVVYLERRGAEYDWRI
                     VPLHGAITPVTGAGCEPDAWMSFSASWPSDPQRLRAFFDDLLAELESMVARADRCRWP
                     LDEPWPHSH"
     gene            10163..11239
                     /locus_tag="VFI55_03060"
     CDS             10163..11239
                     /locus_tag="VFI55_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073045.1"
                     /translation="MNYRITEDDRVRFKRCRRQWDFASPHRRGLEPVGPVHPVVPAVL
                     KDALAVYYYPGTWDWQPEVRRSLVHKAVVRALDDAGPPEAQPTALPALQALVDAYDAW
                     AVTVDDFAPVRIGHDVAGLVPDPRDPERGLTTPDGSPVIYTCRIDLLVVDAGDEYWVV
                     RHLIDDEWPQLGTLIGDEAAVAACWAWEQEYLGMEISGTIHNQVRIAGPLDRPAAGGA
                     ARVSRVQQSEPSGGGRAIPQHRRLSARTSRSDESGHIQEATAGLVRRTRIRRSRADIG
                     AAGELIGAEAVQMSSEPAIYPTFAAHCQACEFIAPCLAMVEGTDPEPLLAAEFRRHPA
                     DDQRKPRLGQSTWGFGRGAAPPQW"
     gene            complement(11250..12377)
                     /locus_tag="VFI55_03065"
     CDS             complement(11250..12377)
                     /locus_tag="VFI55_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HET7073046.1"
                     /translation="MAGGLVSWRSKVRSALTPTEWWRLLAMLSVIVALHLVGWLALVL
                     IVDPARLSLGGKAFGFGVGLTAYTLGLRHAFDADHIAAIDNTTRKLMGDGQRPLAVGF
                     FFSLGHSTVVFALALLLATGVKALVGPVEEDSSTLHHYTGLIGTSVSGVFLYLIAILN
                     VIVLVSILRVFARTRRGGSDAGSDSAMDRAELEQQLQNRGLLNRFLGRFTRSVTKSWQ
                     MYFVGLLFGLGFDTATEIALLVLAGTSAAAGLPWYAVLCLAVLFTAGMCLLDTIDGSF
                     MNFAYGWAFANPVRKIYYNITITALSVAVALLIGTVELLGLFAGQLGWRGPFWDWLGG
                     LDLNTVGFVVVGMFVLTWAVAVLVWRYGRFDEKWNSAETAS"
     gene            12525..13019
                     /locus_tag="VFI55_03070"
     CDS             12525..13019
                     /locus_tag="VFI55_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET7073047.1"
                     /translation="MTPKPSALDPAVTQRIGDFLRARGLRRMASRIQVMAVLEPVNGH
                     LAVAEIHERVRKFLPPGAQPPDVATIYRTVTTLVAQGVLHALTVDGGVTTYGLATAPH
                     HHAVCTQCGSIIEVPARQLRSALEHAMAGSSFALSEAAGLTLHGLCPLCQGDQRDPMS
                     VQAQ"
     gene            complement(13002..14381)
                     /gene="mtr"
                     /locus_tag="VFI55_03075"
     CDS             complement(13002..14381)
                     /gene="mtr"
                     /locus_tag="VFI55_03075"
                     /EC_number="1.8.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050627 - mycothione reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothione reductase"
                     /protein_id="HET7073048.1"
                     /translation="METYDIAIIGTGSGNIILNHRYAHKRAAICEQGTFGGTCLNVGC
                     IPTKMFVYAAEVANTVRDAARYGVDAHVDRVRWDDIVSRIFGRIDPIALSGEDYRRSA
                     PNIDVYGEHTRFGPVQPDGRYLLRTDTGEQFTADQVVIAAGSRPVIPPAILASDVEYH
                     TSDTIMRIAELPEHLVIIGSGFVAAEFAHIFSALGVRVTVVIRGRSMLRHFDDSICER
                     FTRIASAKWELRTHRNVTHAANRGSGVALELDDGCTVDADLLLVATGRSSNADLLDAG
                     QAGIDVQDGRVVVDEYQRTSARDVFALGDVSSPYQLKHVANHEARIVQHNLLCDWDDT
                     ASMAVTDHRFVPSAVFTDPQLATVGLTENQAIARGFDISVAIQNYGDVAYGWAMEDTT
                     GIVKLIAERGSGHLLGAHIMGHQASSIIQPLIQAMSFGLTAAEMARGQYWIHPALPEV
                     VENALLGLH"
     gene            complement(14392..15450)
                     /locus_tag="VFI55_03080"
     CDS             complement(14392..15450)
                     /locus_tag="VFI55_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003414553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7073049.1"
                     /translation="MTEWAPDVLPGYWQRTMPLGPDPAGEGDIVATLIRRGQPTGADH
                     AVLAVHGYTDYFFNTALADHFADRGFAFYALDLRKCGRSRRDGQTPHFITNLAHYDAE
                     LEHALSAIRELPRPGGPGRPAQPISVLVYGHSAGGLIVSLWLDRLHRRDPAAHAAVEG
                     LVLNSPFLDLQGPAVLRHPMTSALISGLSRVRSKGVVRQPTEGGYGTTLHRDYHGEFD
                     YNLQWKPLGGFPITLGWLHAIRHGQARLHRGLDVGVPNLILRSDHTVRETSDPASMQC
                     GDAVLDVSQIARWAGCIGNRTSIVPVPDAKHDVFLSLAQPRETAYRQLDLWLDDHLST
                     DTTDTTDTTEASAPSEKG"
     gene            complement(15539..15925)
                     /locus_tag="VFI55_03085"
     CDS             complement(15539..15925)
                     /locus_tag="VFI55_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET7073050.1"
                     /translation="MPNGNDAPKVSEDSGEPAGLKLYVVTSAARGLAAVQEHLHGHLA
                     YLRELEDRGVLFAAGPLWTDDGEYFTGDGMLIYRAGSISEAQAIADGDPMHTSGARTY
                     RIRPWFLHDGNIGMRVILSQSRHELF"
     gene            16142..17569
                     /gene="mqo"
                     /locus_tag="VFI55_03090"
     CDS             16142..17569
                     /gene="mqo"
                     /locus_tag="VFI55_03090"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HET7073051.1"
                     /translation="MSATLGALLRRLEPDWSITLVERLDTVAAESSGPWNNAGTGHSA
                     LCELNYTPQRPDGSIDITKAVRINEQFQVTRQFWAYAVENGILTDRGFLNPIPHVSFV
                     QGAQRVDYLRRRQRALAGNPLFAGMELIDDPDEFARRLPLMAAKRDFAQPVALNWAED
                     GTDVDFGALSRQLIGFCVRNGTTALFHHEVRNLTRQPDGSWTLLIGNRRTGEKHRLNA
                     KFVFVGAGGDALPLMQKSGIKEIKGFAGFPIGGRFLRASNPALTAAHRAKVYGVPAPG
                     APPLGALHLDHRFVNGKSWLVFGPYAGWSPKFLKHGHFTDLPRSVKSDNLLSLLGVGV
                     SELALVNYLIRQLRLSEPDRVRVLRGFAPSAVDSDWELTVAGQRVQVIRRDKRKGGVL
                     DFNTTLIGAADGSIAGLLGGSPGASTAVPAMLDVLRRCFADRYGSWLPTLKEMVPSLG
                     FELSSEPALYDEVWSRGSKILQLEA"
     gene            17646..18161
                     /locus_tag="VFI55_03095"
     CDS             17646..18161
                     /locus_tag="VFI55_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7073052.1"
                     /translation="MTEALRRAWAKDLDARTLYELLKLRVEVFVVEQACPYPELDGRD
                     LLAETRHFWLETPGGVGVLPSRAGTPPARGGVICTLRLMEEHAGGEKVFRIGRLCTKR
                     SARGQGHTTRLLRAALAEVGDYPCRIDAQTYLADMYAQHGFVRDGEDFVDDGIPHVPM
                     LRPGSHPAAKT"
     gene            18158..19987
                     /locus_tag="VFI55_03100"
     CDS             18158..19987
                     /locus_tag="VFI55_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase subunit D family protein"
                     /protein_id="HET7073053.1"
                     /translation="MKPYPFSAIVGHDQLRLALLLCAVRPEIGGALIRGEKGTAKSTA
                     VRGLAALLSAATGGTDSGLVEMPLGATEDRVIGSLDLQRVLRDGEHAFSPGLLARAHG
                     GVLYVDEVNLLHDHLVDVLLDAAAMGRVHIERDGISHSHDARFVLIGTMNPEEGELRP
                     QLLDRFGLTVEVRASRDVDVRVQVIRQRMAYEADPDAFADRYADADAELAGRIAAARA
                     LVDDVVLPDNELRRIAALCAAFDVDGMRADLVVARTAAAHAAWRGASTVEEQDIRVAA
                     ELALPHRRRRDPFDDPGIDRDQLDEALARAGSEEPQPDPDPPGGGQSVNEPASQPNSH
                     PSSSAQPPRPSAPPSKVFRTRALTVPGVGEGAPGRRSRARNTAGSVVAAADGDDASAH
                     GLHLFATLLSAAEHARAGPLRPRPADVRRAIREGREGNLVIFVVDASGSMAARDRMAA
                     VSGATLSLLRDAYQRRDKVAVITFRQQGARLLLPPTSSAHIAARRLAGFDTGGKTPLA
                     EGLLAARELIVRERARDRARRPLVVVLTDGRATAGPDPLGRSRIASARLLAEGAAAVV
                     VDCETSYVRLGLAYELARQLGAPAVRLEQLHADHLTRAVRSVA"
     gene            20003..20626
                     /gene="cobO"
                     /locus_tag="VFI55_03105"
     CDS             20003..20626
                     /gene="cobO"
                     /locus_tag="VFI55_03105"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET7073054.1"
                     /translation="MPQGRPIHVPDDGLTTRARRNTPVLAVHTGAGKGKSTAAFGMAL
                     RAWNVGLSVAVFQFVKSAKWKVGEEAAFTQLGELHDRHGIGGAVEWHKMGAGWSWTRT
                     SRSEGSELEHAAAAADGWAEISRRLAAQRHDFYVLDEFTYPLKWGWVDVDEVVDVLLA
                     RPGHQHVVITGRDAPPRLVEAADLVTEMTKVKHPMDAGRKGQKGIEW"
     gene            20730..>21457
                     /locus_tag="VFI55_03110"
     CDS             20730..>21457
                     /locus_tag="VFI55_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HET7073055.1"
                     /translation="MTRVPAVVIAAPASGSGKTTVATGLIGALRRAGHTVAPFKVGPD
                     FIDPGYHGLAAGRPGRNLDPVLVGERLIGPLYAHGAGGADIAVVEGVMGLFDGRIGPT
                     QNPVTATPGSTAHVAALLGAPVILVVDARGQSHSIAALLHGFSTFDSATRVCGVILNR
                     VGSPRHEQVLRQACEHAGVPVLGAIPRTAELELPTRYLGLVTVVEYGRRAHVAVEAMT
                     ALVARHVDLAAVFAAAGSRSADPP"
BASE COUNT         3495 a         7096 c         7266 g         3600 t
ORIGIN      
        1 acagcgcagg ccaggtggta accgccgatg cccttgatgg cgaccaccac accggccgcc
       61 agggcgcctt gagtggccgc cagggccgca tccgatgcct gtgtccgttc tcccccttgg
      121 gttgcgcccg ctggacggaa ccacagcgag ggtccgcaat cggggcaggc gatcggctgc
      181 gcgtggaacc ggcgatccga cggatcgtgg tattcggcgg cgcatcgcgc acacatggtg
      241 aatgcggaca tggtggtggc cgggcggtcg tacggcagct cgcggatgat ggtgaaccgc
      301 ggcccgcagt tggtacaggt gacgaacggg tgccggtaac gccggttgtg cgggtcgaac
      361 agctcggcga cacagtcgtc gcacaccgcg acgtcgggcg gaatgggcgt cttaggggtg
      421 tgagcaccgg cgaccgattg gctcgcgacg atccggaact cgcggcggca ggccgtatcg
      481 gcggtgaggt cggtgacggt gacggcgccg atccgggcca gcggcggggc ctcgtgacga
      541 agccgacgat cgaactcgtc gacgcgggcg cgtgcgccct gcacctcgag gaagaccgca
      601 cccgaatcgt tgccgacgaa gccggccagg cccagctcgc tggcgatccg gtggacgaag
      661 ggacgaaagc cgaccccctg aactacaccg gtcaccgtga agtgctggcg gacgacctcg
      721 gaccccggag acggggcaag catcaggggg gagtcggtca tctcagccgt ccccctggct
      781 gacagtcgtg tcggtacggc ccctgcccat cagctcgagg ccggccatcg cttgctcggc
      841 cccggccctg tccgtcttct cgacggcgaa gcccatatgg atgatgatcc aatcacctgg
      901 cgagaacgtc acttccggca acatgccgac gttgaccctg cgctgttcgc cgacgacgtc
      961 gaccagcgcg agctgtccct cgtaaccgtc gagcatccgg atcacttgac cggggatccc
     1021 cagacacatg ctcaatcctc ctgccgcagc ggcgattgca aggtcgcggg agtctgcaac
     1081 gccgcgacga tctcctcgac ggctcgggcg gcgcgcggca cggccttggc caccggctcg
     1141 gtgaggccga tgccctcctc gacgctgccc gcctcgcacc cgatgacgac cgtgtacggc
     1201 gggttgccgc ccagcgctct caggctggcg aacaccgcgg ccggatccat gctgtggccg
     1261 tcgaggccgg ttgcgccgga gccggatacg cggcccggcg cggaaggttc ttcgtggtcg
     1321 gcctggaaga cgtgcagcat ccccggattc ccgcggctgg gcaccgcgtc gacgaggacc
     1381 aacgtgtccc actcctcgag caggtcgtag gccaggtgca tacccctgat gccgtagtcg
     1441 atcacgcgga cactcggatc gttctgcggg atctcggcgt tacggaccac ctccgagccg
     1501 aatccgtcgt ctcccaagaa gatgttgccg atcccggcta ccaggatgcg cgctgtcatc
     1561 gggcttcccg gatgactaca tccgccgcag tcgaaggtag cggcgggcgt cgggcagcga
     1621 caccagtccc agcccgaccg cgactccgag caccagcgcg acgatggcaa cgaatatcca
     1681 acccaacact tccatttcga actcctttca ggggtttacg gtttgttcgg cttcgaccgg
     1741 ctgtatttcg tcgggggaga agtacaggta gcgcccgtac cagtcgtgca gttcggcggc
     1801 ggggtcatcg tcgacgacca ccccgacgtg cttgttgcct tccacgtcct cgtgcaccga
     1861 cgcgacgcgg gcgatcttgc cggcgacgaa gatgtcctgc gcgtcggcgt tgcgtcttgg
     1921 ccgcaacctg acccggctgc cccgggcgac ccggaccccg ttcaccagta ccgcgtcgac
     1981 ttcggggcga acagcgttgt cggccagcgg atcccaccag tcgactccct cggggatctc
     2041 cgggaccagt cccgcggaat caccgggcgc gggtggattc cgcagcacac cgtgcaggcg
     2101 tgccatcgcc tcgggcgaca tggcgtcgca ttggtcgatg atctgcgcgg cccgcggatc
     2161 ggtggcccgg gcctgcgcct tctcctcgtc ggtcatggtc atcacgcgta gcgtgaggat
     2221 ctcgtcgatt tccgtgcagt cataaagcgc ggtgctgctc tgttcggcga cctcggggtg
     2281 gtcgtagagg atgatcggcg agatgagcag aatgtcgtgg gtgccgggcg ggccggccag
     2341 caccgggaag cagcggtggt ggcggcaccg ggacaccgca gcggtggcgg ccggcggcgg
     2401 ctcgagcaac gaaacgaact ggccaccaat gacttcggca atgatgtggg tcccgatcat
     2461 ggaccgcgcg atcgtgtcat tcttgtcggc cccggcagcc ccgacgttgc tcagccgaac
     2521 cgcgaccctg cgcagctccc cgtccggctc gctggacagg ctcaagacgc cacgcaccgc
     2581 acgccgttcg cggaccaggc gcccgccgtc gaccagctcg atatcggtcg acgcgtcggc
     2641 gcgcaccggc agcgtccacg gctgatcgtc gaaggccaag gggccgaacg atcgttcgca
     2701 ttcgaccgcc tcgtcccagg tgagccaaga cccctgccgg gtggtcagct cgtggacggg
     2761 ttcgaactcg ccgtgggcga actcgcgttc ggcgccgcgg tgctgcagct gcaggaaccg
     2821 caccaccagg tttatcgccc gggcaccgtc gaccagaaac tccgctgcca gggtgtcgtc
     2881 ttcacccagc ccggcgtccg ccgcgccggg agggcccaaa acaccgaact gccaacgcga
     2941 ttggttcttg ctcgatgtcc cccggtaggg gtagaggaga tagccctcgt acagcaccgc
     3001 gtcggcgacg gcgcgggccc ggtcccagtt cgaggtggaa gaaatcattg cgccgtctcc
     3061 cgctcggcgt ccaacagcgc ggtgaccgcg tggtccagat cgagcagccc ctgcgcggac
     3121 ttgtagccgg ccagcgccgc gatggtttca tggctcagcc gaacccagcc ggtattcgga
     3181 tagtgctgcg tgatcagctc ccgccaggcc gcgaccggca tgtcatagcg atactcgcag
     3241 tcccagggca cctgctgcac cgagaatccg cgttcggatt tgacgaaaat tgttccgctg
     3301 aacagaaact ggagcggtat ggtgccgtcg cgcagcgcgt gcatgtactt tgcggcggcg
     3361 acctcgaagt cgtaggtgca gtccagcgga aggccgaccg tggtgttgcc ggcgaagccc
     3421 ggcaccatcg tgctgcaatg ttgccagagg aacgtgcgtt gggtggttgc ccaccgctcg
     3481 cgcaggccga acaggtcgat cagcccgtcg gcttcggcgt cggagtaggg ccggcgcagc
     3541 ggttcgatcc gaacctgaca gcgcagcgcg atcgcgtgca ccggatcctc gccgtcggtg
     3601 gcgacgctga cccgggcggt cagcaccggg ctcacgcggt agggctcggg tgccacgtcg
     3661 agaaccgcga agtccacgcc gagctgctgt ccggtcatgg cggcgtcccg gcctgcgtcg
     3721 ttacggcgcg ggcggcgatc tgcgcgaaga agttgtcgat gaaggcccgc gcctcctggc
     3781 caccgtcgaa gccccgccag agcaagcgca agcgcccgac gaactcgtag caggcgtcga
     3841 tcggaaccag atagctttgc ggctgcgcgg cctcgccgtc cgccgggacc cggaccagca
     3901 gcgcctcgac gtcgtcggcc agcaggccca cccgcgaatc cgcgccgctg atggcgttcc
     3961 aggcgtccag gtccagctcg gattcggttg ccccggccgg gcccggatag aacgcgacgg
     4021 tgcggcccag cgcggagttg gtgaagaaga aggccacacc caccgggatc tgcagggcgt
     4081 cccatgcgcg gcggtccagc gcgaagtccg gaaacgccag atagcggtcc ggcaccgccc
     4141 ggtagcgcag ctcggcgccg ccgtcggtga acagcaggta gcaggcccgg cacacgcaca
     4201 tcagctggcg gccggtcacg ttcaccacat gctggtgctc gtcggcaaca agttccgagc
     4261 acatctcgca ccgttcgccg gccggcccgg gggcccgctg gttgcttctg atgcgcgcca
     4321 ggacgtcgta ggcaccagtc atgagcgccg ctcctcctca tcgcttcgtc ccccgcaagc
     4381 gggcggtgcc cccactgcat cgtcgccggc gccggtcatg ccggagcccc catcggttgg
     4441 ccccgcgatg ccaccgacag cacgccgtcg cgggtcaaca gcggaatcgg gtcgaggtgg
     4501 tcacgcgacc cgccagtcac acccgccccg gcgtgcacga cgtcgaaatc cgtctgacag
     4561 cgccggcatc gcagcagggg accgtgcagt tgcgagcccg ccagcgtgtc gtcgcagacc
     4621 gggcaacgat cccgataggc gaacagccgc tcgtcaacac ggcacgccag caccgtggcc
     4681 ccgtcgatcg cgaaacccgc gacctcgccg ggtccgagtt cggccagctc gggcaccgca
     4741 tgccaggtgg tcgcgccgtg accgttggaa ggcccgttcg agtgcacgcg cgccagtagc
     4801 gattcggcgg gaatcatgcc cgcgggagtg gtggcgggtt cggcggccac cacttcgatc
     4861 gcggagatct ccggtgccgc cgcacggacg gcgtcctcga cggccagctc cagggtcacc
     4921 gccgacgacg gacagctctt gcagctgccc cggaaagcaa ggtgcacggt gtcgccggtg
     4981 atgtccagca gatccacgtc gcccccgtgc gagcccaggt acggccgcac ccggtccagc
     5041 gcgtccgaca ctcgccggtg cacgtcatgc gggtgcaggc cgtgcaccag gagcaggctg
     5101 gccaccaggt cgtcggcggc cagccgctcg gcaagagcag ggccctggcc cggagcctga
     5161 gccaacagca tgacccgttc cagcccggcc ccatagagct cgacgacctc gcggaccaac
     5221 tgctgggcgc gttcgtacgc ggtcgccccg ctcgccgcgc aggaatccag cagtgtctgg
     5281 atccgatcgc ccgctgtacg ccacttcgcg tcgctatcgg ggccttcagg gggcgccgtc
     5341 gggcgatcag ccatgcgtca ctccccggcg ggtgactgcg ttggtgagtg cagtctgtcg
     5401 agcgtcttgc cctccccgag gtacatgtgc acaccgcagg gcagacacgg gtcgaagctg
     5461 cgcaccgtgc gcatgacgtc gattcccttg aagttctccc tgctgttctc ctcgaaaatc
     5521 ggctggccct gcaccgcatc ctcgtagggg cccggcgtgc caaagctgtc gcgcgggttg
     5581 gcattccacg gggtcggcgg gtacgggtga tagttggcga tcttgccgtc ccgaatcacc
     5641 atgtggtgac tgagcacacc ccggaccgct tcggtgaagc cgcagccgat ggcctcgtcg
     5701 ggcacctcga acttctccca ggtcttggtg cgtccggcgc ggatctcggc gagcgcctgc
     5761 tcggcgaagt gcagcgcgca cgccgccgca taggcctgga agtaggtacg ggcccggtcg
     5821 cgttcgatcg tgttgctgcc gtgctggggg atcttccact ccagttccac cggccccttc
     5881 aggacggtct tggggaggtt gatcttcacg ctgtttccgg tcgccttgac gtaaccgatg
     5941 tcgaccaggc cggccaacgc cgtcgcccac agccgggcca gcgggccgcc gccggtgtcc
     6001 agcgccaggt gatccttgcc gtcgaaccag cgcggcgaca tcacccagct gtacttgccg
     6061 ccgtccatgt cacgcttctg cgggtgcggg ttggtgtgct ggttccacgg gtggcgccgg
     6121 tccaccgggt tgcccagcgg atcggtcttg acgaacatct cctgttcggt ccagtcgtcg
     6181 taatacgaac tgcccaaaag gattcggata ccgaggttga tgtccaccag cgagtgggtg
     6241 accagctcgc cgtcgaccac cacgccgggt gtgacgaaca tcgcgttgcc ccagcgctcc
     6301 atgtccttgt agttgaagtt gcacacttcg gggtcctgga aggagcccca gcagccgagc
     6361 agggtccgcc gcagtcccac cttctcgtaa ccgggcagcg cctcgtaaaa gaagtcgaag
     6421 aggtcgtcgt gcatggggac gaccttcttc atgaactcga catagcgcat cagccgcgtc
     6481 atgtagtcgg tcatcagctg gatggtcgcg acggtgccga ccccgcctgg gtacagcgtg
     6541 gacggatgca cgtgacgccc ctccataagg cagaacatct ctcgcgtcca gcgactgacc
     6601 tgcagcgctt cccggtagaa ctcaccgctg aacgggttga gcgaccgcat gatgtcggca
     6661 atggtcttgt acccgtgcgc atccgcgtgc ggcgcgggcg ttttctccgc cagtgaaagc
     6721 acgctcgggt tggtctcgga gaccatcttc tcgcagaagt caacgccgac caggttctcc
     6781 tggaagatgt tgtggtcgaa catgtattcc gcggcctcgc cgaggttgac gatccactca
     6841 ccgaggtggg gcggcttgac gccgtaggcc atgttctgcg tgtagcacga gcaggtgcag
     6901 tggttgtcgc cacagatccc gcaaatgcgg ctggtgatga agtgggcgtc gcgaggatcc
     6961 ttgcccttca tgaagatcga gtagccgcgg aagatcgacg acgtgctgtg gcactcgacg
     7021 acttccttgt tctcgaaatc gattttggtg taaatgccga gactgcccac gatcctggtg
     7081 atcggatccc acgccatctc gaccagctgg ccgggttcgc gggtggcatg tgacggctca
     7141 gggatgatcg ttgtcatcgg ccttcgctct ctttagagac gatcaagaaa catgggtggt
     7201 gtcacctgat ggcgctgctt gctcaacgcc ggtgcaccta ccaggtgcga cgggctccgg
     7261 tggtgagttg gtcgcccttg tggcgccagc gcggctcctt gtcgacggtg cgcgccgtga
     7321 taccccgcag gttgcggatc acggtcccgt acagacccga cgcggtgctg gagaatttgc
     7381 cccccggcgg ctcgtccatg aacggcatga acttgtccgg gaaacccggc atcgtgcacc
     7441 cgatgcagat gccgccgacg ttcgggcaac ccccgatgcc gtttatccag ccacgcttag
     7501 gcacgttgca cttcacgacc ggtccccaac aaccaagctt gacaatgcat ttcggtgacc
     7561 cgtactcggt ggcgaaatcg ccctgctcgt agtagccggc gcggtcacaa ccctcgtgca
     7621 cggtgttgcc gaacaaccac ttggggcgca gcgcctcgtc aagcgggatc atcggcgcct
     7681 gaccggtcgc catgtagagc aggtaggtca gcgtctccga aaggttgtcc gggtgaatcg
     7741 ggcagcccgg aacgcagacg atcgggatgc cggccttgct cttccaatcc cacccgaggt
     7801 agtcgggcac gcccatcgcc ccggtcgggt tgccggccat cgcgtggatg ccgccgtagg
     7861 tggcgcacgt tccgaccgcg acgaccgcgg tggctttggg tgttaaccgg tccagccact
     7921 cgctggtggt catcggctgg cccgtggcgg ggttgttgcc gaacccgcac cagtaaccct
     7981 cgtccttgat cttctcgttg gggatggagc cttcgacgac gagcacgaag ggctccaatt
     8041 cgccgcgatc ggccttgaag aaccattcga ggaagtcgtc ggcccctccg gtgggcccgc
     8101 attcgaagtc gatcaagggc cagtgcacgg cgaccttcgg cagacccggg agggcaccga
     8161 gtgcgatttc ctcgacgctg ggctgggtgg cggcagtcaa cgccaccgag tcgccgtcac
     8221 aacttagccc cgcattgatc catagaacgt ggatcagtgt ttcttctgct ttgactgccg
     8281 cttctgttgg catgctgcag cttcccgggg ccgggctgag gcccctacgc cgccggagtc
     8341 gaccatcggc ccacttgcgc agcgctgttt ttgggtctac gcctgcggct caaagttgtc
     8401 aacggttccc acttttcggg caatcaaaca gctgttgcat atcaattctt gccctattat
     8461 cttccgttgt gcggcagtgt atttggacga ttcaaggcgc accgcgtgga gcaatgcggc
     8521 ctggccgaaa ttgtgtgatt ttcacacgat tcgatgcaaa tcatttgcaa caatagggcc
     8581 gtaaatgtat tccctgacgg tgacgccggg ctcagctgtc ggcatcatcg gcgaaccgcc
     8641 gcagccaccc gaaccaggcg cccatgccgg ctccggtgcg cgcactgacc ggcaggatcg
     8701 tcgctgtcgc gttgacctcg cggacgtgtg cgatgtagct gtccacgtcg acgtctagat
     8761 agggcaccaa atcaattttg ttgaggagga cgacgtccac cgatcggaac atcaccgggt
     8821 acttcagcgg cttgtcctcg ccctcggtga cggagtacac catcgccttg gcgtgctcgc
     8881 cgacgtcgaa ttccgccgga cagaccaggt taccgacgtt ctcgacgatc accaggtcca
     8941 ggctcgccag atcgagtccg ggcagggcac ggttgaccat gggcgcgtcc agatggcatt
     9001 cgccgccaaa cccattgctg gtgttcagca gtgacacctg ggcgccgcgg ccgctgagct
     9061 tggccgcgtc cagatccgtc gcgatgtcgc cttcgatcac cccgatcgcg aactcagcgg
     9121 agagctcgtc cagcgttgcc tgcaggatgg tcgtcttgcc cgatcccggt gagctcatca
     9181 ggttgagggt gcggatcccg ttgctttcga acgccgctcg gttggcatcg gcgaggaggt
     9241 cgttctccgc gaagatcgcc tccagcacgt cgatgcgctg tttgcccgtc tcgtagccac
     9301 tgtgatcccc gtggtcgtgc tcacggggaa gatgatcgtg ctcgtgggtg tgcacggttc
     9361 cgtcgtcgtg gcggtgaaat ctacccatgg tcaaatccgt tcacggagag gatctttcat
     9421 gagacgtcca atgacgtcac caggaattcg ttgccgcgta gcacctcgac gtcggcactg
     9481 tcgcacagcg gacaccaaat cgaccaggcc gaggtgatct ccgattggcg gccgcacgaa
     9541 cggcatttca cctcggcgct gacacactcg agttccaact cggcgtccgg catgtcctcg
     9601 gagtcccgca ccagcgtcca gcagaacgac agcgagtccg gtaccacctg gcgaagggcg
     9661 ccgatccgga cacgcaccac gtccacgtgc cggccgtcgg catgggtctt gaccacaccg
     9721 gcgatcgctt cgcacagcga cagctcatgc atcgctgatc accggcctcg gatcaagggc
     9781 aggcccatga gcactgttct acacccgaat cgtggttatc ctgaacctca cggggcgcag
     9841 cgtgaaggtt gacgagttat tcgcctcgat cgaggcgcac cgccccggtc gcgatgacgt
     9901 cgtgtacctc gagcgccgtg gcgccgaata tgactggcgg atcgtgcccc tgcacggcgc
     9961 gatcaccccg gtcaccgggg ccgggtgcga acccgacgcg tggatgtcgt tctcggcgtc
    10021 gtggccgtcg gacccgcagc ggttgcgggc gttcttcgat gacctgctcg ccgagttgga
    10081 atccatggtc gctcgtgcgg atcgctgtcg ctggccgctc gacgagccct ggccgcactc
    10141 gcattgagcg gcggcggcca tcgtgaacta tcggatcacc gaagacgacc gcgtccggtt
    10201 caagcgctgt cggcgacagt gggatttcgc ctcgccgcac cgccgagggc tagagcccgt
    10261 cgggccggtg catccggtgg tgcctgccgt gctcaaggat gcgctggccg tctactacta
    10321 cccgggcacg tgggactggc aacccgaggt gaggcgatcg ctggtgcaca aggccgtggt
    10381 ccgggcgctg gacgatgcgg gcccgccgga ggcgcaaccc accgcccttc ccgccctgca
    10441 ggcgctggtc gacgcctacg acgcctgggc ggtgacggtc gacgacttcg ctccggtgcg
    10501 gatcggccac gacgtcgcgg ggctggtgcc cgacccgcgc gaccccgagc gcggcttgac
    10561 gaccccggac ggatcccccg tgatctacac ctgccggatc gatctgctgg tcgtcgacgc
    10621 cggcgacgaa tattgggtgg tgcggcacct gatcgacgac gaatggccgc agttggggac
    10681 gctgatcggt gatgaggcgg cggttgcggc gtgctgggcc tgggaacagg aatacctggg
    10741 catggagatc tcgggcacca ttcacaacca ggtgcgcatc gccggcccgc tcgatcggcc
    10801 tgccgccgga ggcgccgcga gggtaagccg ggttcagcag agcgagccca gcggcggcgg
    10861 tcgtgcgatc ccgcagcatc ggcggctgtc ggcgcgtacg tcacgaagcg acgagtcggg
    10921 ccatattcag gaggcgaccg ccgggctggt gcggcgaact cgcatccggc gaagccgcgc
    10981 cgacatcggc gccgccggcg agctgatcgg tgccgaggca gtgcagatga gcagtgagcc
    11041 ggcgatctat ccgaccttcg ccgcccactg tcaggcgtgc gaattcatcg ccccgtgcct
    11101 ggccatggtc gagggaacag acccagaacc gctattggcg gcagagtttc gccggcaccc
    11161 ggccgacgat cagcgaaagc cgcgactggg tcagtcgaca tggggcttcg gtcgcggcgc
    11221 cgcaccgccg cagtggtgaa cggcggcggc taactcgcgg tttcggccga attccacttt
    11281 tcgtcgaagc gcccgtaacg ccagaccagc accgcgaccg cccaggtcag gacgaacatg
    11341 ccgacgacga cgaagccgac ggtgttgagg tccaggccgc ccagccagtc ccagaagggg
    11401 ccccgccagc cgagctggcc cgcgaacagg ccgagcagtt cgacggtgcc gatcagcagc
    11461 gcgacggcca ccgacagcgc cgtgatggtg atgttgtagt agatcttgcg caccgggttg
    11521 gcgaaggccc agccgtaggc gaagttcata aacgaaccgt cgatggtgtc cagcagacac
    11581 atgcccgcgg tgaacagcac ggccagacac agcacggcgt accacggcag gccggccgcc
    11641 gcgctggtgc ccgcgaggac cagcagcgcg atttcggtgg cggtgtcgaa ccccagcccg
    11701 aacagcaatc cgacgaaata catctgccag gacttggtga ccgacctggt gaagcggccg
    11761 aggaagcggt tgagcaatcc gcggttttgc aattgctgct cgagttcggc gcggtccatg
    11821 gcggagtcag agccggcgtc agagccgccg cggcgcgtcc gcgcgaacac gcgcaggatg
    11881 gacaccagga cgatgacgtt gaggatggcg atcagataca ggaacacgcc cgacacgctg
    11941 gtgccgatga ggcccgtgta gtggtgcagc gtcgaggaat cctcctcgac cggtccgacg
    12001 agggccttga ccccggtcgc cagcagcagt gccagcgcaa agaccaccgt ggagtggccc
    12061 agcgagaaga agaagccgac ggccaggggc cgctgcccgt cgcccatcag cttgcgggtg
    12121 gtgttgtcga tggcggcgat gtggtcggcg tcgaacgcgt gccgcaggcc caaggtatag
    12181 gccgtcaacc cgacgccgaa gccgaatgcc ttgccgccca ggctgagtcg tgccggatcc
    12241 acgatcagca ccaaggcgag ccagccgacc agatgcagcg cgacgatcac cgacaacatc
    12301 gctaggagtc gccaccactc cgtcggcgtc agggcgctgc gcaccttcga ccgccatgaa
    12361 acgagcccac cggccatgtc ggtccttcca gcattgggca gacacaacag ccaaaactgt
    12421 aggttcgtcc gccgaccgga tgcaagtgac ttgcaggaaa cgcaccggcc cctgttgccc
    12481 cggggcactg gtaatcgggg ggtgtccggc gcgacactga atgggtgacg cccaagccgt
    12541 cggctctcga ccccgccgtc acccagcgca tcggtgactt cctccgtgct cgcgggctgc
    12601 gaaggatggc ctcacggatc caggtgatgg cggtgctcga gccggtcaac gggcacctcg
    12661 ccgtggcgga gatccacgag cgggtgcgga agtttctgcc gcccggcgcg cagccccccg
    12721 acgtggcgac catttatcgc acggtgacga ccctggtcgc ccagggcgta ttgcacgcgt
    12781 tgaccgtcga cggtggcgtc accacctacg ggctggccac cgccccccac caccacgcgg
    12841 tgtgtacgca atgcggctcg atcatcgagg tgcccgcacg gcagctccgc tcggcgctcg
    12901 agcacgcgat ggcgggcagc tcgttcgcgc tgtcggaggc ggcgggcctg acgctgcacg
    12961 ggctgtgccc gctgtgccag ggcgaccagc gtgatccgat gtcagtgcag gcccagtagc
    13021 gcgttttcga ccacctcggg cagcgccgga tggatccagt attgcccgcg ggccatctcg
    13081 gcggcggtca gcccgaagct catcgcctgg atcagcggct ggatgatcga cgatgcctgg
    13141 tggcccatga tgtgggcgcc cagcaagtgg ccgctgccgc gctcggcgat gagtttcacg
    13201 attccggtgg tgtcttccat cgcccagccg taggcgacgt cgccgtagtt ctgtatcgct
    13261 accgagatgt cgaatcctcg tgcgatggct tggttttcgg tcaacccgac ggtcgccagc
    13321 tgggggtcgg tgaacaccgc cgaggggacg aagcggtggt cggtcacggc cattgacgcg
    13381 gtgtcgtccc agtcgcacag caggttgtgc tgcacgattc gggcctcgtg attggcgacg
    13441 tgtttgagct gatacggcga agagacgtca ccgagtgcga aaacatcgcg tgccgacgtc
    13501 cgttggtatt cgtcgaccac cacccggccg tcctggacgt cgatgccggc ttgtccggcg
    13561 tccagcaggt cggcgttgga cgaccggccg gtcgcgacca gcagcaggtc ggcgtcgacg
    13621 gtgcaaccat cgtcgagctc cagcgcgacg ccggagccgc gattggcggc atgcgtgacg
    13681 ttgcggtgcg tgcgcagctc ccatttggcc gaggcaatgc gggtgaatcg ttcgcaaatg
    13741 ctgtcgtcga aatgccgcaa catgctccga ccacggatca ccacggtgac tcgtacgccg
    13801 agagcggaga agatatgcgc gaattccgct gccacgaagc cacttccgat gatcaccagg
    13861 tgttcgggca actcggcgat ccgcatgatg gtgtcgctgg tgtggtactc gacgtcggat
    13921 gcgaggatcg ccggcgggat caccgggcgc gagcccgccg cgatcaccac ctggtccgcg
    13981 gtgaattgtt cgccggtgtc ggtacgcaac aggtagcgcc cgtcgggttg taccggtccg
    14041 aaccgggtgt gctcgccgta tacgtcgatg ttgggtgccg agcgccggta gtcttcgccg
    14101 ctgagcgcga tggggtcgat ccgtccgaag atgcgggaga cgatgtcgtc ccagcgcacc
    14161 cggtcgacgt gcgcgtcgac gccgtaccgt gccgcatccc ggacggtgtt ggcgacctct
    14221 gcggcgtaga cgaacatctt cgtcgggatg cacccgacgt tcaggcaggt gccgccgaag
    14281 gtgccctgct cgcagatcgc cgctcgtttg tgggcatagc gatggttcaa aatgatgttg
    14341 cccgaaccgg ttccgatgat cgcgatgtcg taggtttcca tttttgcgtc gtcacccctt
    14401 ttccgagggt gccgaggctt cggtggtatc ggtggtatcg gtggtatcgg tgctcaggtg
    14461 gtcatccagc cagaggtcca gttgccgata ggccgtctcc cgcggctgcg ccaacgacag
    14521 gaacacatcg tgtttggcgt ccggcaccgg cacgatgctg gtgcgattcc cgatacagcc
    14581 ggcccacctg gcgatctggc tgacgtcgag gaccgcgtcg ccgcattgca tggacgccgg
    14641 atcgctggtt tcgcgcacgg tgtgatccga acgcaggatc agattcggca cccccacgtc
    14701 gagtccgcga tgcagccgtg cctggccgtg acgtatggcg tgcagccacc cgagggtgat
    14761 cggaaagccg cccaacggtt tccactgcag gttgtaatcg aactcgccgt ggtaatcgcg
    14821 gtgcagggtg gtgccgtagc cgccttcggt gggttggcgg accacaccct tggaccgcac
    14881 tcgagacagg ccggagatca gcgccgaggt catcgggtga cgtaggacgg ccgggccctg
    14941 cagatccagg aacgggctgt tgaggaccag gccctcgacg gcggcgtgtg ccgccgggtc
    15001 gcgccggtgc aaccggtcca gccacaacga cacgatgagc cccccggcgg aatggccata
    15061 caccaggacg ctgatgggct gggcgggtcg gccgggtccg ccgggacggg gtagctcgcg
    15121 gatggctgac agcgcgtgtt cgagttcggc gtcgtaatgg gccaggttgg tgatgaagtg
    15181 tggggtctgg ccgtcgcgcc gcgaccggcc gcacttccgc aggtccagcg catagaacgc
    15241 aaagccgcga tcggcgaagt gatcggccag tgcggtgttg aagaagtagt ccgtgtaccc
    15301 gtgcaccgcg aggaccgcat gatcggcccc ggtgggctgg ccgcgtcgta tcagggtcgc
    15361 gacgatgtcg ccctcgccgg ccggatcggg cccgagcggc atcgtgcgct gccaatagcc
    15421 gggcagcaca tcgggcgccc actcggtcac cccgtctgtc acggggccag cttagagggt
    15481 ggtcaccgga cgtcagtgcc gacggcaaga acggatcgga ttcgccaccc ttatgtcctc
    15541 aaaagagctc gtggcgagac tgcgagagga tcactcgcat accgatattc ccgtcgtgga
    15601 ggaaccacgg tctgatccgg taggttcgcg cgccgctggt gtgcatggga tccccgtcgg
    15661 cgatggcctg ggcctcgctg atcgaaccgg cgcgatagat caacatgccg tcgcctgtga
    15721 agtactcgcc gtcatcggtc cacagcggtc cggcggcgaa gagcactccg cggtcttcca
    15781 gctcacgcag gtaggcaagg tggccgtgca gatgttcttg caccgcggcc aatccgcgtg
    15841 ccgcggaggt gacgacgtag agtttcaacc cggccggttc gcccgaatcc tcgctgactt
    15901 tcggcgcgtc gtttccgttg ggcaccatca actccttgtc cttggtcgtt cttgagtggc
    15961 gatgcgacct gtcgattccg cgtaacggtc cgcccgcgaa gtggcacttt gcgaagccgg
    16021 cctgcggtga cgcttcctat cgggataacc tgggagacca gcatagggaa ggaccagcga
    16081 tccggtgtca gagctagcca gaaccgcacg gaccgatgtc gtgctggtcg gcgcgggcat
    16141 catgagcgcg acgctgggtg cattgctaag gcggctggag ccggattggt cgatcaccct
    16201 ggtcgagcgt ctcgacacgg tcgccgccga aagcagcggt ccctggaaca acgccggcac
    16261 cgggcactcg gcgctgtgcg agctcaacta caccccacag agacccgacg gctcgatcga
    16321 catcaccaaa gcggtacgca tcaacgagca gttccaggtg acccgccaat tctgggcgta
    16381 cgccgtcgag aatggcatcc tgaccgatcg cggtttcctc aacccgatcc cgcacgtgag
    16441 cttcgtgcag ggggctcagc gggtcgatta tctgcgccgt cggcagcggg cgctggccgg
    16501 caacccgctg ttcgccggta tggagctcat tgacgacccg gacgagttcg cccgccgctt
    16561 gccgttgatg gccgccaagc gtgacttcgc tcaacccgtt gcgctcaatt gggccgaaga
    16621 cggcaccgat gtcgacttcg gagcgttatc ccgacagctc atcggctttt gcgtgcgcaa
    16681 cggcaccact gcgctgttcc atcacgaggt ccggaacctg acccgccagc ccgacggcag
    16741 ctggacgctg ttgatcggca accgccgcac cggcgaaaag caccggctca acgcgaagtt
    16801 cgtgtttgtc ggcgccggtg gcgacgcgtt gccgctgatg cagaagtcgg gcatcaagga
    16861 gatcaagggt ttcgccggct tccctatcgg cggccggttc ctgcgcgcca gcaacccggc
    16921 gctcaccgcc gcacaccgag ccaaggtgta tggcgttccg gcaccgggag ccccgccgct
    16981 cggcgcgctg catctggacc accggttcgt caacggcaag tcctggctgg tgttcgggcc
    17041 gtacgccggt tggtcaccga agttcttgaa acacggccac ttcaccgatc ttccccgctc
    17101 ggtcaagtcg gacaacttgc tgtcgttgct gggcgtcggc gtaagcgagt tggcgttggt
    17161 caactatttg atcaggcaat tgcggctgtc cgaacccgac cgggtgcggg tactgcgcgg
    17221 attcgccccc agcgcagtcg attcggactg ggagctgacg gtcgccggtc agcgtgttca
    17281 ggtcatccgg cgcgacaagc gcaaaggggg ggtgctcgac ttcaacacca cattgatcgg
    17341 tgccgctgac ggcagcatcg ccggactgct gggcggctct ccaggcgcgt cgacagccgt
    17401 accggccatg ctcgacgtgc tgcggcgatg ctttgccgac cgctatgggt cgtggctgcc
    17461 cacactcaaa gagatggtgc cgtcgctggg ttttgagctg tcaagcgaac ccgcgctcta
    17521 cgacgaagtg tggtcgcggg gatccaagat attgcagctg gaggcctgac gacctgcgac
    17581 ggcgacgatg cagtgggggc accgcccgct cgcgggggac gtggcgatga ggaggagcgg
    17641 cgcccatgac ggaagcactg cgtcgcgcgt gggccaaaga ccttgatgcc cggactcttt
    17701 acgagctact caagttgcgg gtggaggttt tcgtcgtcga gcaggcctgt ccgtatccgg
    17761 agctggacgg gcgcgacctg cttgccgaaa cccggcattt ctggctggaa acgcctggcg
    17821 gcgtgggggt actccccagc agagcgggga ccccacccgc tcgcggaggc gtgatctgca
    17881 cactgcggtt gatggaggag cacgcgggcg gggagaaggt gttccggatc ggccggttgt
    17941 gcaccaaacg cagtgcgcgc ggtcagggcc acaccactcg gctgctgcgg gcggcactgg
    18001 cagaggtcgg cgactacccc tgccggatcg acgcgcagac ttatctggcc gacatgtacg
    18061 cccaacacgg attcgtgcgt gacggagagg atttcgtcga cgacggtatt ccgcatgtgc
    18121 ccatgctgcg gcccggctcc catccggcgg ccaagacgtg aagccatatc cgttcagcgc
    18181 gattgtcggc cacgatcaac tgcggctggc gctgttgttg tgtgcggtgc ggccggagat
    18241 cggtggtgcg ctgatccgcg gcgagaaggg caccgccaag tcgaccgccg tgcgagggct
    18301 cgcggcgctg ctgtccgccg cgaccggcgg caccgactcg ggcctggtcg aaatgccgct
    18361 cggcgcaacc gaagaccggg tcatcggttc gctggacctg cagcgggtcc tgcgcgatgg
    18421 cgagcatgcg ttctcacccg gactgctggc ccgcgcacac gggggcgtgc tctacgtcga
    18481 cgaggtcaac ctgttgcacg accatctggt cgacgtgctg ctcgacgccg cggccatggg
    18541 acgggtacac atcgagcgcg acgggatctc gcattcgcac gatgcccggt tcgtcctcat
    18601 cggcaccatg aatccggagg agggcgagct gcgtccgcaa ctgctggacc ggttcggtct
    18661 caccgtcgag gtgcgcgcgt cgcgcgacgt agacgtccgg gtgcaggtta tccggcagcg
    18721 aatggcctac gaggccgacc cggacgcgtt cgcggatcgt tacgccgacg ccgacgcgga
    18781 gctggccgga cggatcgccg cggcgcgcgc tctcgtcgac gatgtggtgt tgccggacaa
    18841 cgaattacgg cgcatcgcgg cgctgtgcgc ggcgttcgac gtcgacggca tgcgggccga
    18901 tctggtggtg gcccgcaccg ccgcggccca cgcggcctgg cgcggcgcga gcacggtcga
    18961 ggagcaggac atccgggtgg ccgccgagct ggcgttgccg caccggcgtc ggcgagatcc
    19021 cttcgacgat cccggcatcg accgcgacca gctggacgaa gccctggctc gcgcgggttc
    19081 tgaagaaccg cagcccgatc cggacccgcc gggtggcggt cagtccgtga atgagccggc
    19141 ttcccaacca aactcacacc cctcatcgag cgcgcaacca ccgaggccca gcgcgccgcc
    19201 gtcgaaggtc ttccgcaccc gcgcgctgac cgttccgggc gtcggcgaag gcgcaccggg
    19261 acgacggtcg cgggcccgca acaccgcagg cagcgtggtg gccgccgccg acggcgacga
    19321 cgcaagcgct catggcctgc acctgttcgc gaccctgctg tcggccgccg agcatgctcg
    19381 ggcggggccg ctgcggccac gacctgcgga cgtgcgccgg gcgatccgcg aaggacgcga
    19441 aggcaacctg gtgatcttcg tcgtcgacgc gtcgggttcg atggccgccc gcgatcgcat
    19501 ggccgcggtc agcggcgcca ccctgtcact gctgcgcgac gcctaccagc gccgcgacaa
    19561 ggtcgcggtg atcacgttcc gccaacaggg ggcacggctg ctcctcccgc ccacgtcgtc
    19621 ggcgcacatc gccgcgcgcc gcctggccgg cttcgacacc gggggcaaga ctccgttggc
    19681 cgaaggcctg ctggccgccc gtgagctgat cgtccgggag cgggcgcgtg accgggcccg
    19741 tcgtcccctg gtggtggtgc tcaccgacgg ccgggccacc gccggaccgg acccgttggg
    19801 ccgcagcaga atcgcgtcgg cgcggctgct cgccgaaggc gcggccgcgg tggtcgtcga
    19861 ctgcgagacg tcctatgtgc ggctcgggtt ggcttacgaa ctcgcccgtc agctcggtgc
    19921 gcccgccgtg cggctggagc agctgcacgc cgaccacctg acgcgggccg tgcgcagcgt
    19981 ggcctgagaa aggtaagtgc ttatgccaca aggccgtccg atccacgtgc ccgatgacgg
    20041 cctgaccacc cgggcccggc gcaatacgcc cgtgctggcg gtacacaccg gtgcgggcaa
    20101 ggggaagtcg acggcggcat tcggaatggc gttgcgcgcc tggaatgtcg gcctcagcgt
    20161 ggcggtgttc cagttcgtca agagcgccaa gtggaaggtg ggcgaggagg cggccttcac
    20221 gcaactcggc gagttgcacg accggcacgg gatcggggga gccgtcgaat ggcacaagat
    20281 gggtgcgggc tggtcctgga cgcgtacgtc gcgtagcgag ggcagcgaac tcgagcacgc
    20341 agcggccgcg gccgacggct gggccgagat ctcccgccgg ctggccgcgc agcggcacga
    20401 cttctacgtg ctcgacgaat tcacctatcc gctgaagtgg ggttgggtcg atgtcgacga
    20461 ggtggtggac gtcctgctgg ccaggcccgg tcaccagcat gtggtgatca cgggacgcga
    20521 cgccccgccg cgcttggtcg aggccgccga cctggtcacc gagatgacca aggtcaagca
    20581 tccgatggac gcgggacgca agggccagaa gggcatcgag tggtgacccc gccttcggcc
    20641 ggcgagcgtg cgcgtctgta caacgacacg ccgtataccg tggcattttg cgcacgctcg
    20701 cggcactggg tcacgcagtg ggccgcaagg tgacccgagt ccccgcggtg gtcatcgccg
    20761 cgcccgcatc gggaagcgga aagaccaccg tggcaacggg tttgatcggg gctttacgac
    20821 gggcgggcca tacggtcgcg ccgttcaagg tcgggccgga cttcatcgac cccggctatc
    20881 acggcctggc cgccggacgg cccggccgca acctcgaccc ggtattggtg ggggagcggc
    20941 tcatcggccc gctctacgcc cacggcgccg gcggcgcgga catcgccgtg gtcgaaggcg
    21001 tgatggggct gttcgacggg cgcatcggac ccactcaaaa tccagtgacc gccacgcccg
    21061 gttccaccgc gcacgtcgcc gccctgctgg gcgccccggt gatcctggtc gtcgacgcgc
    21121 gcggccagag tcacagcatt gcggctctgc tgcacggctt ttcgacgttc gatagcgcga
    21181 cccgggtctg cggtgtgatc ctcaaccgcg tcgggtcgcc cagacacgag caggtgctgc
    21241 gtcaggcctg cgaacacgcc ggcgtccccg tgctgggcgc catcccgcgc accgctgaat
    21301 tggagctgcc gacaaggtat ctcgggcttg tcacggtcgt cgagtacggc cgtcgggcgc
    21361 acgtcgcggt cgaggcaatg accgcactgg tcgcccgcca cgtcgatctg gccgcggtgt
    21421 tcgcggccgc cggatcccgg tccgccgatc cgccatg
//
LOCUS       DASSGQ010000071        17947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1463110,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000071 DASSGQ010000000
VERSION     DASSGQ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 17947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17947
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463110"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VFI55_03115"
     CDS             <1..305
                     /locus_tag="VFI55_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HET7073056.1"
                     /translation="PVGFDPNIPMPPAPDVPPPPPVHAYSVNWDAIAQCESGGNWSIN
                     TGNGYSGGLQFTSGTWHANGGSGSAAGASREEQIRVAENVLHSQGIHAWPVCGRRG"
     gene            461..1717
                     /locus_tag="VFI55_03120"
     CDS             461..1717
                     /locus_tag="VFI55_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020725990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-acting enoyl reductase family protein"
                     /protein_id="HET7073057.1"
                     /translation="MTATPREFDIVLYGATGFVGKLTAEYLARAAAGARIALAGRSQD
                     RLLAVRESLAEPAQSWSVLTADAASPSTLDEMAARTQVVVTTVGPYTRYGLPLVAACA
                     AAGTDYADLTGEPPFIGDSIDLYHKQAADNGARIVHACGFDSIPSDLSVYALYRAARD
                     DGEGELAGTDFVVRSFSGGLSGGTIASLVEVLNAASSDPDVRRRLADPYSLSTDRGAE
                     PELGRQPDLPWRRGRQLAPELARQWTAGFMMAPSNTRIVRRTNALLDWAYGRQFRYSE
                     NMSLGSSPIAPLASAVVTGIGNATFGLGGRYFRLLPRRLVERILPKPGTGPSAAARDR
                     GYYRVETYTTTTTGARYVARMEQQGDPGYKATSVLLGESALALAFDRDSLSELRGVLT
                     PASAMGDALLARFPAADVSLRTERLS"
     gene            1872..2441
                     /locus_tag="VFI55_03125"
     CDS             1872..2441
                     /locus_tag="VFI55_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020786722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HET7073058.1"
                     /translation="MAGLDDLYSQIPISDIANKLGASQGDVEKAVHTLVPALLTGMQQ
                     NAEDPENVSKIESAATSHAARGLLDVGGGEGGDGHQAVASLFGGNDADQVASALADRG
                     AGNRDLLKQLLPVVLPIVLAYIGKQLGSGGASSAPQKTASGGGLGDILGSILAGGSGD
                     KSLGGILGNVLSGKGGLGDILGGLLGGKK"
     gene            2609..3202
                     /locus_tag="VFI55_03130"
     CDS             2609..3202
                     /locus_tag="VFI55_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET7073059.1"
                     /translation="MADTLTAGQKLVRGDSIASNNGAYTLTLQDDGNLVLASRGHAIW
                     STATDGQDVVRAEVQPDGNFVLYTPDKPVWHTDTKGKKDVRLVLQDDRNLVLYAGDGA
                     AWSTKTETDAPPPPEPVAEEPVAEEAAGEAEPVAEAAPEAAPEPEVRTYTVVSGDSLW
                     AIAERFYGDGSKYQVIADASGIPNPDLIQPGQVLTIP"
     gene            3312..5972
                     /locus_tag="VFI55_03135"
     CDS             3312..5972
                     /locus_tag="VFI55_03135"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET7073060.1"
                     /translation="MTASHNPAADLPKSWEPGVMEQAIYQRWLDAGYFTADPASAKPA
                     YSIVLPPPNVTGSLHMGHALEHTMMDALTRRKRMQGYEVLWQPGMDHAGIATQTVVEK
                     QLAVDGKTKEDFGRELFVDKVWDWKRESGGAIGGQMRRLGDGVDWSRDRFTMDEGLSR
                     AVRTIFKRLYDAGLIYRAERLVNWSPVLQTAISDLEVNYQDVDGELVSFRYGSMDDAE
                     PHIVVATTRVETMLGDTAIAVHPDDERYRHLVGSSLPHPFVDRPLAIVADEHVDPEFG
                     TGAVKVTPAHDPNDFEIGLRHQLPMISIMDTKGRITGTGTQFDGMDRFEARVAVREAL
                     AAQGRVVAEKRPYLHSVGHSERSGEPIEPRLSLQWWVRVESLAKAAGDAVRNGDTVIH
                     PASLEPRWFAWVDDMHDWCISRQLWWGHRIPIWYGPNGEQVCIGPDDTPPEDWEQDPD
                     VLDTWFSSALWPFSTLGWPEKTPELEKFYPTSVLVTGYDILFFWVARMMMFGTFVGDD
                     DAITLGGLRGPQVPFTDVFLHGLIRDEFGRKMSKSKGNVIDPLDWVETFGADALRFTL
                     ARGASPGGDLAVGEDAVRASRNFGTKLFNATRYALLNGAVLAPLPAQDELTDADRWIL
                     GRLEQVRAEVDSAFDSYEFSRACEALYHFAWDEFCDWYLELAKSQLADGLAHTTAVLA
                     AVLDTLLRLLHPVIPFITEALWQALQNGETNQESLVVADWPTASGVDMDPVAAQRITD
                     MQRLVTEVRRFRSDQGLADRQKVPARLTGVEESDLTGQVPAATSLAWLTAPGPDFRPS
                     ASLEVRLSRTTVVVELDTSGTIDVAAERRRLEKDLALAQKELASTAAKLANADFLAKA
                     PEQVVGKIRERQRVAQEETDRITSRLAGLQ"
     gene            5969..7432
                     /locus_tag="VFI55_03140"
     CDS             5969..7432
                     /locus_tag="VFI55_03140"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HET7073061.1"
                     /translation="MTEPPEWESATGPETGTAPTPDEIASLLQVEHLLDQRWPETKIE
                     PSLTRISALMDVLGSPQLSYPSIHIAGTNGKTSVARMVDALVTALQRRTGRTTSPHLQ
                     SAIERIAIDGRPISPAHYVATYREIEPFVQMIDAQSEASGAGPRLSKFEVLTAMAFAA
                     FADAPVDVAVVEVGMGGRWDATNVINAPVAVITPIGIDHVDYLGDDIAGIAAEKAGII
                     TRAPDGAPDTVAVIARQVPEAMEVLLAQSVRADAAVAREDAEFAVLGRQVAIGGQVLQ
                     LQGLGAVYSDVYLPLHGEHQAHNAVVALAAVEAFFGAGAQRQLDVEAVRAGFAAVRSP
                     GRLERMRSAPTVFIDAAHNPAGAAALAQTLTTEFNFRFLVGVLSVLADKDVDGILAAL
                     EPVFDAVVVTHNGSPRALDVESLALAAGERFGPDRVTTAGNLRDAIDIATALVDDASM
                     DTADGADGGGFSGTGIVITGSVVTAGAARTLFGRDPE"
     gene            7429..7797
                     /locus_tag="VFI55_03145"
     CDS             7429..7797
                     /locus_tag="VFI55_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4233 domain-containing protein"
                     /protein_id="HET7073062.1"
                     /translation="MTDSPGDQSPAPRADPWKSFSAVMAATLILEAIVVLLAIPVVGA
                     VGGGLTAGSLAYLLGLAAVLILLAGLQRKPWAIWVNMGVQAILLAGFAVYPGVGFIGV
                     LFTGLWALIAYFRAEVRRRQ"
     gene            7852..8262
                     /gene="ndk"
                     /locus_tag="VFI55_03150"
     CDS             7852..8262
                     /gene="ndk"
                     /locus_tag="VFI55_03150"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET7073063.1"
                     /translation="MTERTLVLIKPDAVERHLIGEIISRIEQKGLTIAALELRHTSEE
                     LAVQHYAEHDGKPFFASLLEFITSGPVVAAIVEGPHAVAAFRQLAGGTNPVEQATPGT
                     IRGDFGLETQFNLVHGSDSADSAKREIALWFPGA"
     gene            8604..11468
                     /locus_tag="VFI55_03155"
     CDS             8604..11468
                     /locus_tag="VFI55_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HET7073064.1"
                     /translation="MADGAPPSEPTQEQTRPEDLPDRLRVHQLARALGSTNREVLEAL
                     NTLDGRPRNVHSGVDREDALRVRDLLFAKPLPNIPGFSSAPVAQQPLLLPTSTDRPQY
                     MPLFVAPQPLETADDDDVDDDSGDDSDSDDDEEQTDRPANRRRRRGRRGRGRGRGEQG
                     GSDGGDDDGEPGRRGQGPAQADAESEDTDGRDSDEEDDADSGDDENGLSEAGSRRRRR
                     RRRRKSGSGDDNDEGLSPDDPPNTVVHERPPRSGQNGSDGGGSNGSGSTEIKGIDGST
                     RLEAKRQRRRDGRDAGRRRPPVLTEAEFLARREAVERMMVVRDRVRTEPPHPGARYTQ
                     IAVLEDGIVVEHFVTSAASASLVGNIYLGIVQNVLPSMEAAFVDIGRGRNGVLYAGEV
                     NWEAAGLGGSDRRIEQALKPGDYVVVQVSKDPVGHKGARLTTQVSLAGRYLVYVPGAS
                     STGISRKLPDTERQRLKEILREVVPANAGVIIRTASEGVKEDDIRNDVNRLQERWQQI
                     EAKAAETKQKAGGAAVALYEEPDVLVKVIRDLFNEDFAGLLVSGDEAWNTINEYVNSV
                     APELVSKITKYEPAAGSDGATGPDVFAVHRIDEQLAKAMDRKVWLPSGGTLVIDRTEA
                     MTVIDVNTGKFTGSGGNLEQTVTKNNLEAAEEIVRQLRLRDVGGIVVIDFIDMVLESN
                     RDLVLRRLTEALARDRTRHQVSEVTSLGLVQLTRKRLGTGLIEAFSTSCPNCGGRGIL
                     LHADPVDSAPVNGRKSEPGGGRRSRRSKKGRPEEPAVAKVPVHTPGEHPMFKAMAAGS
                     TGVSEEADRGDEESEEAGDEATAKLDVIAKLDGETEERAVADLEDADQEDFEDSEDDT
                     DDDDEDELDDEDLDDEDLDDDDDDDDDDDEDLDEDLDDDDDDDLDEVDSDPGPDEHSN
                     AGTGNDLAQSTAPGITGSPFGRPRRRRAAGRPAGPPIHVD"
     gene            11693..12004
                     /gene="rplU"
                     /locus_tag="VFI55_03160"
     CDS             11693..12004
                     /gene="rplU"
                     /locus_tag="VFI55_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET7073065.1"
                     /translation="MATYAIVKAGGKQYKVAVGDVVKVEKIESEPGAQVSLPVALVVD
                     GATVTTDAAALAKVAVTGEVLEHTKGPKIRIHKFKNKTGYHKRQGHRQRLTVLKVTGI
                     K"
     gene            12020..12286
                     /gene="rpmA"
                     /locus_tag="VFI55_03165"
     CDS             12020..12286
                     /gene="rpmA"
                     /locus_tag="VFI55_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET7073066.1"
                     /translation="MAHKKGASSSRNGRDSNAQRLGVKRFGGQVVKAGEILIRQRGTK
                     FHPGVNVGRGGDDTLFAKEAGAVEFGVKRGRKTISIVAAGQSTD"
     gene            12406..13848
                     /gene="obgE"
                     /locus_tag="VFI55_03170"
     CDS             12406..13848
                     /gene="obgE"
                     /locus_tag="VFI55_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET7073067.1"
                     /translation="MPRFIDRVVIHARAGSGGNGCASVHREKFKPLGGPDGGNGGRGG
                     SIVLVVDPQVHTLLDFHFHPHVTAPSGKQGMGNNRDGAAGADLEVHVPDGTVVLDENG
                     RMLADLVGAGTRFEAAAGGRGGLGNAALASRARKAPGFALLGEAGQSRDLTLELKTVA
                     DVGLIGFPSAGKSSLVSAISAAKPKIADYPFTTLVPNLGVVSAGEHTFTVADVPGLIP
                     GASAGRGLGLDFLRHIERCAVLVHVVDCATAEPGRDPISDIDALEAELAAYTPTLQGD
                     AALEDLAERPRAVVLNKIDVPEARELAEFVRDEIAERGWPVYLVSTVTREGLQPLIFG
                     LWQMISDYNAARPQTVPRRPVIRPVPVDDSGFTVEPDGEGGFVVTGARPQRWVGQTNF
                     DNDEAVGYLADRLARLGVEEELLRLGARPGCAVTIGEMTFDWEPQTPAGQQVALSGRG
                     TDARLERSDRVGAAERKAARRRRREHGDGS"
     gene            13904..15007
                     /gene="proB"
                     /locus_tag="VFI55_03175"
     CDS             13904..15007
                     /gene="proB"
                     /locus_tag="VFI55_03175"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET7073068.1"
                     /translation="MTEHREAIRTARSLVVKVGTNALTTRSGVFDAGRLAGLADAIEA
                     RMKAGTDVVIVSSGAIAAGIEPLGLSRRPKDLATKQAAASVGQVALVNSWSAAFARYG
                     RTVGQVLLTAHDISMRASHTNAQRTLDRLRALHAVAIVNENDTVATNEIRFGDNDRLS
                     ALVAHLVGAEALVLLSDIDGLYDSDPRKTKGAKFIPEVSGTADLDGVVAGPGSDLGTG
                     GMASKMSSALLAADAGVPVLLAAATDAATALTDASTGTVFAARSDRMSARRFWVRYAA
                     EAAGALSLDEGAVRAVVLQRRSLLPAGITTVSGRFYAGDVVELRDPDATMVARGVVAY
                     DATELATMMGRSTSELPGELRRPAVHADDLVAV"
     gene            complement(15012..15662)
                     /locus_tag="VFI55_03180"
     CDS             complement(15012..15662)
                     /locus_tag="VFI55_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7073069.1"
                     /translation="MPSVTRKPQANREQRRQQRREQMERRLLDAAERLMRDGASFTEL
                     SVDRLSTEAGISRASFYVYFEDKGHLLRRLAGQVFADLAASANRWWSVAWRHNPDDVR
                     VAMDGIVATYRRHQPVLVALNEMASYDPVVGATYRNLLTAITGRVTRVIEQGQSDGSI
                     RSELPAATTASALTWMVERACQQNLPTAPPSYDAELAASMTEIIWGTLYLKPVPPS"
     gene            15766..17043
                     /locus_tag="VFI55_03185"
     CDS             15766..17043
                     /locus_tag="VFI55_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7073070.1"
                     /translation="MSVMTGTSGTEKPGRAYDAIDLSSRAFWSTTAADRERSFAVLRA
                     ERPVSWHPPVEDALMHDPDDPGYWAITRRSDIVAVSRNNEVFLSGQGVLFENIPVELL
                     EASQSFLAMDPPRHTKLRKVVSAAFTPRQVRRIEDSIKSNAKTIVEELRAAGSGADFV
                     DRCAKEVPIRTLSDMVGIPESERERMAHATDALVSWADPVYLDGRNPLEVLLENQMYL
                     HQVAGMLAAQRREHPGDDLLSSLVNAEVDGDRLTDADVAAFFVLLSVAGNDTTRQTIS
                     HAMKALTDFPGERAWLLEDFDNRIGTAVEEFIRWATPVMTFRRTAATDSELGGQRIRA
                     GEKVVMFYPSGNWDREAFDHPDRFDLSRSPNPHLGLGGGGVHFCLGASVGRAQLRAIF
                     GELLTQLPDIQAGDPAYVPGNFIHAIRSLPCTF"
     gene            complement(17040..17909)
                     /locus_tag="VFI55_03190"
     CDS             complement(17040..17909)
                     /locus_tag="VFI55_03190"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HET7073071.1"
                     /translation="MTARCAESPELVALLAGRRVAAVTGAGISTDSGIPDYRGPDSPP
                     SNPMTIRQFTSDPVFRQRYWARNHVGWRHMHDTLPNAAHRALAALERASVVTGVITQN
                     VDLLHTKAGSLNVVNLHGTYARVICLGCSYGMSRATLAERLEALNPGFIERAEAVGGL
                     AVAPDADAVVADTASFRYLDCPRCDGMLKPDIVYFGESVSKAVVAQAYSLVDQAEALL
                     VAGSSLTVFSGYRFVRRAAALGIPVAIVNRGHTRGDNLAAVKVDSGCSELLALLASEL
                     VGAPARAPAAATR"
BASE COUNT         3056 a         5873 c         6208 g         2810 t
ORIGIN      
        1 caccggtggg tttcgacccg aacattccga tgcctccggc accggacgtt ccgccgccgc
       61 ccccggtgca cgcctacagc gtgaactggg acgccatcgc gcagtgcgag tcgggtggaa
      121 actggtcgat caacaccggt aacggctact ccggtggcct gcagttcacg tcgggcacct
      181 ggcacgccaa cggtggctcg ggttcggcgg ccggcgccag ccgcgaggag cagattcggg
      241 tggccgagaa cgtcctgcac agccagggca ttcacgcttg gccggtctgc ggtcggcgtg
      301 gctgatcagc ggctgcgcct gacggcctga cagccgtcgt caagtaatac cgtcgttaag
      361 tgatagagga atgccgggcc ctgtgcccgg catttctttg ttttcttgcc cgttgcgccc
      421 accggttacg cctgacctcc gtcggggtag cgtcggaccc gtgaccgcaa cgccgcgcga
      481 gttcgacatt gtgttgtatg gggcgaccgg cttcgtgggg aagctgaccg ccgaatacct
      541 ggcgcgggcc gcagcgggcg cgcggatcgc gttggccggc aggtcccagg accgattgct
      601 cgccgttcgc gagagccttg cggaacccgc gcagtcttgg tcggtcctga ccgccgacgc
      661 ggcatcgccg tcgacgctgg acgagatggc cgcccggaca caagtcgtcg tcaccacggt
      721 cgggccttac acccgctacg ggctgccgct ggtggccgcg tgtgccgcgg cgggcaccga
      781 ttacgccgac ctgaccggcg agccgccgtt catcggcgac agcatcgacc tgtatcacaa
      841 gcaggccgcc gacaacggcg cccgcatcgt gcacgcctgc ggattcgact ccatcccctc
      901 ggacttgagc gtgtacgcgc tgtaccgggc ggcgcgagac gacggcgagg gcgagctcgc
      961 cggcaccgac ttcgtggtgc gctcattctc gggtgggcta tccggtggca ccatcgcgtc
     1021 gctggtggaa gtgctgaacg ccgcctccag cgaccccgac gttcgccgcc ggcttgccga
     1081 cccgtactcg ttgagcaccg accgcggcgc cgaacctgag ctcggccgcc aacccgacct
     1141 gccatggcgt cgcggtcgcc agctcgcgcc ggaactggcg cgtcagtgga cggcgggatt
     1201 catgatggcg cccagcaaca cccgaatcgt gcggcggacc aacgccctgc tcgactgggc
     1261 atacggccgc caattccgct acagcgaaaa catgagcctc gggtcgtcgc ctatcgcgcc
     1321 attggcatcg gccgtcgtca ccgggatcgg caacgccacc tttggattgg gcggccgtta
     1381 tttccggctg ctgccccgtc ggctggtgga gcgcattctc cccaaacccg gcaccggccc
     1441 gagtgcggcg gcccgggacc gtggctacta ccgggtcgag acctacacca ccacaaccac
     1501 cggtgcccgt tatgtagcac gcatggagca gcagggggac cccggttaca aggcgacctc
     1561 ggtgctgtta ggcgagtcgg ctctggcgct ggcattcgac cgcgactcgc tgtcggagct
     1621 gcgcggcgtg ctgacgcccg cttcggcgat gggggacgcc ttgttggcgc gattcccggc
     1681 cgccgacgtc tcactgcgaa ccgagcggct gagctgactc tcgcggcggt gcggggtgcg
     1741 gaaggtccga gcagtcgacc tacggccggg agttaactcc gtcctgggag aaggctgtcg
     1801 accaacaccg catgcgtcta gtgtcgtaat ggacttggct ttacatcctg ttaacgaagg
     1861 tggacacatc gatggccggt ctcgacgatc tgtacagcca gattcccatc tctgatatcg
     1921 ccaacaagct cggcgccagc caaggcgacg tcgagaaggc tgttcacacg ctggtgcccg
     1981 cactgcttac cgggatgcag cagaacgcgg aagaccccga aaacgtaagc aagatcgaat
     2041 ccgccgccac cagtcacgcc gcacgcggtc tactcgacgt cggcggtggc gaaggcggcg
     2101 acgggcacca ggcggtcgcg tcgctgttcg gtggcaatga cgccgaccag gtcgcgtcgg
     2161 cgctggccga tcggggagcc ggcaatcgtg atctgctcaa gcagctgttg ccggtggtgc
     2221 tcccaattgt gctggcatac ataggaaagc agctcggttc cggcggcgcg agcagtgcgc
     2281 cgcagaaaac ggcgtcgggc ggcggcctgg gtgacattct gggcagcatc ctcgctggtg
     2341 gctccggcga caaatccctt ggcggcatcc tcggcaatgt gttgagcggc aagggaggac
     2401 tcggcgatat cctcggcggc ctgctcggcg ggaagaagta gcgcgacgcg cgtccggatt
     2461 gttcacgggt agttcccgga ttggtttcga ttgcgtcgaa ttcaaggctg cccaagacaa
     2521 atcaaggtaa gtaggctcgg ttctatcgaa gatgggcaat ggcgtccgac ccgagtcggc
     2581 cgggcgtcga cgacgagagg gagatgccat ggcggacacg ctcactgcag gacaaaagct
     2641 cgtgcgaggt gactcgatcg cctcgaacaa cggcgcctac accctcacgt tgcaagacga
     2701 cggcaacctg gtgctggcgt cccgcggcca cgcgatctgg tccacggcga ccgacggcca
     2761 ggatgtggtg cgcgccgaag tgcaacccga cggcaacttc gtcctctaca cgccggacaa
     2821 accggtatgg cacacggaca ccaagggcaa gaaggacgtc aggctggtgc tgcaggacga
     2881 ccgcaacctg gtgctctacg ccggtgacgg cgcggcctgg tcgaccaaga ccgagactga
     2941 cgccccgccg ccgcccgagc ccgtcgccga agagcctgtc gccgaggaag ccgctggcga
     3001 ggcggagccc gttgcggaag cagctcctga ggcggccccg gagcccgagg ttcggacgta
     3061 cacggttgtc tcgggcgaca gcctgtgggc gatcgcggag cgtttctacg gcgacggcag
     3121 caagtaccag gtgatcgccg atgccagcgg aatccctaac cccgacctga tccagcccgg
     3181 ccaggtgctg accattccct gatcggcacg gctggcggcc cccatcgaat cgcaagtatt
     3241 cgcgagaatt cgcgtgacct gcggattcgg gcgcttacac gcgcgtcaat gcgtctccct
     3301 agaattgacc ggtgaccgcc agccataacc ctgccgccga cctgcccaag tcgtgggaac
     3361 ccggcgtgat ggagcaggcg atctatcagc ggtggctgga cgcgggctat ttcacggcgg
     3421 atccggccag cgccaaaccc gcgtactcga tcgtgctgcc gccgccgaac gtgaccggca
     3481 gcctgcacat gggccacgcg ctggaacaca cgatgatgga cgccctgacc cgccgcaagc
     3541 ggatgcaggg ctacgaggtg ctgtggcagc cgggcatgga ccatgcgggc atcgcgacgc
     3601 aaaccgttgt ggaaaagcag cttgcggtcg acggcaagac caaggaggac ttcggccgcg
     3661 aactgttcgt cgacaaggtg tgggactgga agcgtgagtc gggtggcgct atcggcggcc
     3721 agatgcgccg gctcggcgac ggcgtggact ggagccgcga ccggttcacc atggacgagg
     3781 gcctgtcgcg ggcggtgcga accattttca agcgcctcta cgacgcgggg ctcatctatc
     3841 gggcggagcg gctggtcaac tggtcgccgg tgcttcagac ggcgatctcc gacctagagg
     3901 tcaattacca agacgtcgac ggcgagctgg tgtcgttccg gtacggctcc atggacgacg
     3961 cggaacccca catcgtggtc gccaccaccc gagtcgagac gatgttgggc gacaccgcga
     4021 tcgcggttca tccagacgac gagcgctacc gccacctggt cggcagcagc ctgccgcatc
     4081 cgttcgtcga ccgcccgctc gctatagtcg ccgacgaaca tgtcgacccc gaattcggca
     4141 ccggcgcggt caaagtcacg cccgcgcacg acccgaacga cttcgaaatc gggctgcggc
     4201 accagttgcc gatgatctcg atcatggaca ccaagggccg gatcaccggc accggaacgc
     4261 aattcgatgg catggaccgc ttcgaggcgc gagtcgcggt gcgcgaagct ctggcggcgc
     4321 aaggacgcgt ggtcgcggag aagcgcccct acctgcacag cgtcgggcat tctgagcgca
     4381 gcggcgagcc gatcgagccg cggttgtcgc tgcagtggtg ggtccgggtg gagtcgctgg
     4441 ccaaggccgc cggcgatgca gtgcgtaacg gggacactgt gattcacccc gccagcctgg
     4501 aaccccgctg gttcgcgtgg gtcgacgaca tgcacgactg gtgtatctcg cggcagctct
     4561 ggtggggcca tcgcatcccg atctggtacg ggcccaacgg cgaacaggtg tgcatcggtc
     4621 ccgacgacac acctccggag gactgggaac aggaccccga cgtgctggac acctggttct
     4681 cgtccgcgct gtggccgttc tccacgttgg gctggccgga gaagactccc gaactggaaa
     4741 agttttatcc gacaagtgtt ctcgtgaccg gatacgacat cctgttcttc tgggtcgcgc
     4801 ggatgatgat gttcggcacc ttcgtgggcg atgacgacgc aatcaccctc ggcggcctgc
     4861 gcggcccgca ggtgccgttc accgatgtct tcctgcacgg cctcatccgt gacgaattcg
     4921 gccgcaagat gagtaaatcc aagggcaacg tcatcgatcc gctggattgg gtcgagacgt
     4981 tcggggccga cgcgctgcgg ttcacgctgg cccgcggcgc cagtcccggc ggtgatctgg
     5041 ccgtcggcga ggatgccgtc cgcgcgtcgc gcaacttcgg caccaagctg ttcaacgcca
     5101 cccgctacgc actgctcaac ggtgccgtac tggcaccgct gcctgcgcag gatgagctga
     5161 ccgacgccga ccgatggatc ctggggcggc tggaacaggt tcgcgccgaa gttgattcgg
     5221 ccttcgacag ctacgagttc agtcgcgcgt gcgaggcgct ctaccacttc gcgtgggacg
     5281 aattctgtga ctggtacctc gaattggcca agtcgcaact tgccgacgga ctggcgcaca
     5341 ccaccgcggt gctggccgcg gtgctggata cgttgctgcg gctgctgcat ccggtgatcc
     5401 cgttcatcac cgaggcgctg tggcaggctt tgcagaatgg ggagaccaac caggagtcac
     5461 tggtggtcgc agactggccg accgcgtcgg gcgttgacat ggatcctgtt gctgcacaac
     5521 gcatcacgga catgcaaagg ctggtgaccg aggtgcggcg gttccgcagc gaccagggcc
     5581 tcgccgatcg gcagaaggta ccggcccgac tgaccggcgt cgaggagtcc gacctcacgg
     5641 gccaagtgcc cgccgcgacc tcgctggcgt ggctcaccgc accgggcccc gacttccgcc
     5701 cgtccgcgtc gctggaggtc cggctcagcc gcaccaccgt cgtcgtcgaa ctcgacacct
     5761 cgggcaccat cgacgtggcc gccgaacgtc gccgcctgga aaaggacttg gccttggcgc
     5821 aaaaggaatt ggcgtcgacg gccgccaagc tggccaacgc ggacttcctg gccaaggcgc
     5881 cggagcaggt cgtcggcaag atacgtgagc ggcagcgggt ggcacaggag gaaaccgatc
     5941 ggatcacctc caggttggcc gggctgcaat gactgagccg cccgagtggg aatcggcgac
     6001 cggacccgaa accggcaccg ctcccacacc ggacgagatc gcgtccctgc tgcaggtcga
     6061 gcatctgctg gatcagcgct ggccggagac caagatcgag ccgagcctga cccgcatcag
     6121 cgcactgatg gacgtgctgg gctcgccaca actcagttat ccgtcgatcc acatcgcggg
     6181 caccaacggt aagacctcgg tcgcgcggat ggtggacgcg ctggtgaccg cgctgcagcg
     6241 gcgcaccggg cgaaccacca gcccacacct gcaatcggcg atcgaacgca tcgcgatcga
     6301 cggccggccg atcagcccgg cccactacgt ggcgacctac cgcgagatcg agccgttcgt
     6361 gcagatgatc gacgcgcagt cggaggcttc tggggctggc ccgcgcttga gcaagttcga
     6421 ggtcctcacg gcgatggcgt tcgccgcgtt cgccgacgcg cccgtcgacg tcgcggtggt
     6481 cgaggtgggc atgggcgggc gttgggatgc gaccaacgtg atcaacgcac cggttgcggt
     6541 gatcaccccg atcggcatcg accacgtcga ctatctcggc gacgacatcg ccgggatcgc
     6601 cgcggagaag gccggcatca tcaccagggc tcccgatggg gcaccggaca ccgtcgccgt
     6661 catcgcgcgt caggtgcccg aggcgatgga ggtgttgttg gcccaatcgg tgcgcgccga
     6721 cgccgcggtc gcccgcgagg acgcggagtt cgcggtcctg ggccgccagg tcgccatcgg
     6781 agggcaggtg ctgcagctgc agggtctggg tgcggtgtac tccgacgtgt acctgccgct
     6841 gcatggcgaa caccaagcgc acaacgcggt ggtggcgttg gccgcggtcg aggccttctt
     6901 cggcgccggt gcccagcgtc agctcgacgt cgaggccgtt cgggcgggtt tcgccgccgt
     6961 caggagcccc ggccggctgg aacgaatgcg cagcgcccca acggttttca tcgacgccgc
     7021 gcacaatccg gccggggcgg cggcgctggc gcagacgttg accacggagt tcaacttccg
     7081 atttctggtc ggcgtgctca gcgtgctggc cgacaaggat gtcgacggca tcctcgccgc
     7141 gctggagccg gtgttcgatg ccgtcgtcgt gacccacaac ggctcgccgc gggcgctgga
     7201 cgtggaatcg ctggcactgg cggcgggtga gcggttcgga cccgaccggg tgacgaccgc
     7261 cgggaacctg cgcgacgcca tcgacatcgc gaccgccctg gtcgacgacg cctccatgga
     7321 caccgccgat ggcgccgacg gcggcgggtt ctccggcacg gggatcgtca tcaccgggtc
     7381 ggtggtcacc gccggcgccg cacggaccct gtttggtcgt gatccggaat gaccgacagt
     7441 ccgggagacc agtccccggc gccccgggcc gatccgtgga agagcttcag cgccgtgatg
     7501 gccgcgacgc tgattttgga agcgatcgtg gtgctgctgg cgattccggt ggtcggcgcg
     7561 gtcggcggcg gcctgaccgc cggttcgctg gcctatctgc tggggctggc cgcggtgctg
     7621 atcctgcttg ccggattgca gcgcaaaccc tgggcgatct gggtgaatat gggcgtgcaa
     7681 gcgatcctgc tcgcggggtt tgcggtgtac ccgggtgtgg gtttcatcgg ggtgttgttc
     7741 accggcctgt gggcgttgat cgcctacttc cgcgccgaag tgcggcgacg gcagtagcgt
     7801 ccagccagct acgccgggct aatccgcacg gggttctgta cgctgtccgc cgtgaccgaa
     7861 cggaccctgg tactgatcaa gccggacgcc gtcgagcggc acctgattgg cgagatcatc
     7921 agccgcatcg agcaaaaagg cctcaccatc gcggcgctgg agctccggca taccagcgaa
     7981 gagctggccg tccagcatta cgcggagcat gacggcaagc ccttcttcgc gtcgttgctg
     8041 gagttcatca cttcggggcc cgtggtcgcg gcaatcgtgg aaggaccgca cgcggttgcg
     8101 gcgttccgcc agctcgccgg cggcaccaac ccggtggaac aggcgacgcc cggcacgatt
     8161 cgcggtgatt tcgggctgga aacgcagttc aatctggtgc acggctcgga ttcggccgat
     8221 tccgccaagc gcgaaatcgc gctctggttc cccggcgcct agggttggac ggcaggcgcc
     8281 cgtagcttcg gccccggtcc tggttcgcgg agccttcgga tcaggggcgg gtgagcgcgc
     8341 ccggtcatgt gggatactta cagcgggtga acgtcgccgg accggcgtca gacacccgaa
     8401 tgaagactta gacaagcgcg accatcgcca ccccgcgatg ttgcgccgca tgtcaggccg
     8461 tcattcagca cggcgccgag cgagttctac ccgggctgct cggggcaaga ccctcacaag
     8521 cctgggagaa gtgaccctgg catataacga agccctcgcg tggccgcgtc gcaacgacgc
     8581 gcccgggggc taaaggagaa tacgtggcag acggtgcacc accttcagaa ccaacacagg
     8641 agcagacccg gcccgaggac ctgccggacc ggctgagagt ccatcagctg gcacgcgcgc
     8701 tgggcagcac caacagggag gtgctggaag cgctcaatac cctcgacggg cggccccgca
     8761 acgtgcattc cggcgtcgac cgcgaggacg cgctccgggt gcgtgacctg ctcttcgcca
     8821 agcccctgcc aaacatcccc ggcttcagca gcgcaccggt cgcccagcaa ccgctgctgc
     8881 tgccgacctc gaccgaccgg ccgcagtaca tgccgctgtt cgtcgccccg caaccgctcg
     8941 agacggccga tgacgatgac gtcgacgacg actcgggcga cgactccgac agcgacgacg
     9001 acgaggagca gaccgaccgg ccggcgaacc ggcgtcggcg tcgtggtcgt cggggtcgtg
     9061 gccgaggacg tggtgaacag ggcggatcgg acggcggtga cgacgatggc gagcccggcc
     9121 gccggggaca gggtccggcc caggccgacg cggaatccga ggacaccgac ggccgagact
     9181 ccgacgagga agacgacgct gactccggcg acgacgagaa cggcttgtcg gaagcgggga
     9241 gccggcgccg ccgcaggcgt cggcgacgca agtcgggatc cggtgacgac aacgacgagg
     9301 gtctgtcgcc cgatgatccg ccgaacaccg tggtgcacga gcgcccgccc cgcagcggcc
     9361 agaacggttc cgatgggggt ggctccaacg gctccggtag caccgagatc aagggcatcg
     9421 acggctccac tcggctagag gccaagcggc agcgccgacg ggacgggcgc gacgccggac
     9481 gtcgccgccc gccggtgctg accgaggccg agttcctggc gcgccgcgag gccgtcgaac
     9541 ggatgatggt ggtccgcgac agggtccgca ccgagccgcc gcaccctggc gcgcgctaca
     9601 cccagatcgc ggtgctcgaa gacggcattg tcgtcgaaca cttcgtaacg tcagcggcct
     9661 cggcttccct ggtgggcaac atctacctgg ggatcgtgca gaacgtgctg ccgtcgatgg
     9721 aggcggcctt cgtcgacatc ggtcgcggcc gcaacggcgt gctctacgcg ggcgaagtca
     9781 attgggaagc cgcgggattg ggcgggtctg accgcaggat cgaacaagcg ctcaaacccg
     9841 gcgattacgt cgtggtccag gtcagcaagg acccggtggg gcacaagggt gcgcggctca
     9901 ccacgcaggt gtcactggcg gggcgctacc tggtctatgt gccgggcgcc tcgtcgaccg
     9961 ggatcagtcg caagctgccg gacaccgaac gtcagcggct caaggagatc ctgcgtgagg
    10021 tcgtaccggc gaacgccggg gtgatcatcc gcacggcgtc cgagggcgtc aaagaggacg
    10081 acatccgcaa tgacgtcaac cgcctgcagg agcgctggca acagatcgag gccaaggcgg
    10141 ccgagaccaa gcagaaggcg gggggcgccg cggtggcgct ctacgaggag ccggacgtgt
    10201 tggtcaaggt catccgtgac ctgttcaacg aggacttcgc cgggctcctg gtgtccggcg
    10261 acgaggcgtg gaacacgatc aacgaatatg tgaattcggt tgcgcccgaa ctggtttcga
    10321 agattacgaa atacgagccc gcggcggggt cggatggcgc gacgggaccg gacgtgtttg
    10381 cggtacatcg catcgacgag cagctcgcca aggccatgga ccggaaggtg tggctgccgt
    10441 cgggtggcac gcttgtcatc gaccgcaccg aggcgatgac ggtgatcgac gtcaacaccg
    10501 gcaagttcac cggttccggg ggaaacctcg agcagaccgt gaccaagaac aacctggagg
    10561 cggccgagga gatcgtgcgc cagctacggc tgcgcgacgt cggcggcatc gtggtcatcg
    10621 actttatcga catggtgctt gagtccaacc gtgacctggt gttgcgtcgg ctgaccgagg
    10681 cgctggcccg cgaccgcacc cgccatcagg tctccgaggt gacgtcgctg ggcttggtcc
    10741 aactgacccg caagcggttg ggaaccgggc tgatcgaggc gttctcgaca tcgtgcccga
    10801 actgcggcgg ccgcgggatc ttgctgcacg ccgacccggt cgattcggct ccggtgaacg
    10861 ggcgaaagtc cgagccgggc ggtggccgcc gaagcaggcg atcgaagaag ggcaggcccg
    10921 aagagcctgc cgtggccaag gtgccggtgc atacccccgg cgagcacccg atgttcaagg
    10981 cgatggccgc cggctcgacc ggggtgtcgg aggaagccga tcgcggtgac gaggaatccg
    11041 aggaggccgg tgacgaagcc accgccaagc tcgacgtcat cgccaaatta gatggcgaga
    11101 ccgaagagcg ggcggtcgcc gacctggaag acgccgacca ggaggacttc gaggactccg
    11161 aagacgacac cgacgacgac gatgaggacg aactcgacga tgaggacctc gacgatgagg
    11221 acctcgacga cgacgatgac gatgacgatg acgacgacga ggatctcgac gaagacctcg
    11281 atgacgacga cgatgacgat ctcgatgaag tggattccga ccccgggccc gacgagcaca
    11341 gcaatgccgg gaccggtaat gacctcgccc agagcaccgc gccgggcatc accgggtcgc
    11401 cttttggccg cccgcgacgt cggcgcgcag caggaaggcc ggccggaccg cccatccacg
    11461 tggactgacc ccggcccggt tgggcaccgg atcagcagcg gttgcagctg gtttgaccct
    11521 gtagccgctg gtcaagtacc cttggacagt tgtcgtcagg cctggactgg ccgtcgactg
    11581 gcgggactgc cggaaagtcg gcgcagaccc gcacccagac ccaccacgca cacaccggtg
    11641 gcacgcgcgc gacgcgccga gccggcggaa caagaaagag gcaggagcaa cgatggcgac
    11701 ctacgcaatc gtcaaggcgg gcggcaagca gtacaaagtc gccgtcggag acgtggtcaa
    11761 ggtcgagaag atcgaatccg agcccggcgc gcaggtgtcg ctgccggtcg ccctggtcgt
    11821 cgacggtgcc accgttacca ccgacgccgc cgcgctggcc aaggtcgcgg tgaccggtga
    11881 ggtgctcgag cacaccaagg gccccaagat ccgtatccac aaattcaaga acaagaccgg
    11941 ctaccacaag cgtcagggtc accgtcaacg gctgacggtc ctgaaggtca ccggcatcaa
    12001 gtagcgaggc gacgaacaga tggcacacaa gaagggcgcg tccagctcac gcaacggtcg
    12061 cgattccaac gctcagcggc tgggcgtcaa gcggttcggt ggccaggtcg tcaaggccgg
    12121 cgaaatcctg atccgccagc gcggcaccaa gttccatccc ggcgtcaacg tcggacgtgg
    12181 cggcgacgac acgctattcg ccaaggaagc cggagctgtc gagttcgggg tcaagcgcgg
    12241 gcgcaagacg atcagcatcg tcgcggccgg ccagagcacc gactgagctt cttccccggg
    12301 ccgtgctggc ccgcatcgtc gccgggctga ggttaggatt gctcggctcc tcagcgggcc
    12361 gtggtggccc gtatcgtcgc cgggctgaag gtgggagggc actcgatgcc tcggtttatc
    12421 gatcgcgtcg tcatccacgc gcgcgcgggt tcgggtggta acggctgcgc ctcggtccat
    12481 cgggagaaat tcaagccgct tggcggccct gacgggggca acggcggccg cggcggcagc
    12541 atcgtcctcg tcgtcgaccc gcaggtgcac accctgctgg acttccattt ccatccccac
    12601 gtcaccgcac catccggcaa gcagggaatg ggcaacaacc gcgacggcgc ggccggcgcg
    12661 gacctggagg tgcacgtccc cgacggcacc gtcgtgctgg acgaaaacgg ccgcatgctg
    12721 gccgatctgg tgggcgcggg cacccgcttc gaggccgcgg ccggcggccg tggcggtctc
    12781 ggcaacgccg cgctggcatc ccgcgcccgc aaggccccgg gcttcgcgct gctcggcgag
    12841 gcgggccagt cccgcgacct gacactggag ctcaagaccg tcgccgacgt cggcctgatc
    12901 gggttcccgt cggccggcaa gtcttcgctg gtgtccgcga tttcggcagc taagccgaag
    12961 atcgccgact atccgttcac cacgttggtc cccaacctcg gtgtcgtctc ggcgggcgag
    13021 cacaccttca ccgtcgccga cgtgcccggc ctgatcccgg gtgcttcggc gggccgcggc
    13081 ctgggcctgg atttcctgcg gcacatcgag cgctgtgccg tgctggtgca cgtcgtcgac
    13141 tgcgccaccg cggagcccgg acgcgacccg atttccgaca tcgacgcact ggaagcggaa
    13201 ctcgccgcgt atacgcccac cctgcaaggg gatgcggcgt tggaggacct cgccgagcgg
    13261 ccccgggccg tggtgctcaa caagatcgac gtgcccgagg ctcgtgagct cgccgaattc
    13321 gtccgcgacg agatcgccga acgtggctgg ccggtgtatc tggtgtcgac cgtgacgcga
    13381 gaaggcctgc agccgttgat cttcgggctc tggcagatga tctccgacta caacgccgcg
    13441 cggccgcaaa ccgtcccgcg acggccggtg attcgtccgg tgcccgtcga tgacagcggc
    13501 tttacagtcg aaccggacgg agagggcggc tttgtcgtca ccggtgcgcg gccccagcgc
    13561 tgggtcggcc agaccaattt cgacaacgac gaagccgtgg gctatctcgc cgaccggctg
    13621 gcgcgtctgg gtgtcgaaga ggaactgctg cggctgggcg cgcgaccggg gtgcgccgtg
    13681 acgatcggag aaatgacgtt cgactgggag ccccaaacgc ccgcgggaca acaggtggcg
    13741 ttgtcgggac gcggtaccga cgcgcggctg gagcgcagcg atcgcgtcgg cgccgccgag
    13801 cgcaaggccg cccggcgtcg gcgccgcgaa cacggtgacg gctcgtgact gtgtcggcga
    13861 cggtgcaggg ggcaccgccc gcttgcgggg gagagtggcg ctcgtgactg agcatcgcga
    13921 agccatccga acggcgcgca gcctggtggt caaggtcggg accaacgcgc tgaccacacg
    13981 gtcgggagtg ttcgacgccg gccggctggc cgggctggcc gacgcgatcg aggcgcgaat
    14041 gaaggcgggc accgacgtcg tcatcgtgtc gtccggagcc atcgccgctg gcattgagcc
    14101 gctcggattg tcgcgccgcc caaaggatct ggcgaccaag caggccgcgg ccagcgtcgg
    14161 gcaggtcgcc ctggtgaact cgtggagcgc ggcgttcgcc cgttacggcc gcaccgtcgg
    14221 tcaggtgctg ctgaccgcac acgacatttc gatgcgggcc tcgcacacca acgcccagcg
    14281 cacgctggac cggctgcgcg cgttgcacgc ggtggcgatc gtgaacgaga acgacaccgt
    14341 ggccaccaac gagatccggt tcggcgacaa cgaccggctt tccgcgctgg tggcccacct
    14401 ggtcggagcc gaggccttgg tgctgctctc cgacatcgat gggctgtatg actccgaccc
    14461 tcgaaaaacc aagggggcca agtttattcc tgaagtgtcg ggtacggctg acctggacgg
    14521 cgtggtcgca ggtccgggca gcgacctggg caccggcgga atggcgtcga agatgtcgtc
    14581 ggcgctgctg gcggccgacg ccggggtccc ggtgttgctg gccgccgcga ccgacgccgc
    14641 gacggcgctc accgacgcgt caaccggcac tgtgttcgcc gcgcgctcgg atcggatgtc
    14701 ggcccgccgg ttctgggtgc ggtatgccgc cgaggcggcc ggtgcgctgt cgctcgacga
    14761 gggcgcggtg cgcgctgtgg tgctgcaacg ccgctcgctg ctgcccgccg gcatcaccac
    14821 cgtctcgggc cgattctatg cgggagatgt cgtcgaattg cgcgacccgg acgcgacgat
    14881 ggtggcccgt ggtgtggtcg cctacgatgc gaccgaactc gcgaccatga tgggccggtc
    14941 gacctccgag ctgcccgggg agctgcgccg gcccgccgtg cacgccgatg acctggtcgc
    15001 cgtctgacaa ttcagctcgg cggcactggc ttgagataca gtgtgcccca aatgatttca
    15061 gtcatcgacg cggccagctc ggcatcgtag gaaggcggag cggtcggtag attctgctgg
    15121 cacgcgcgct cgaccatcca ggtcagcgca ctggctgtgg tggccgcggg cagctcactg
    15181 cggatggagc cgtccgactg gccctgttcg atgacgcggg tcacccgtcc ggtgatggcg
    15241 gtcaacagat tgcggtaggt cgctccgacc acggggtcat aggaagccat ctcgttcagg
    15301 gcgacgagca cgggctgatg acgccggtag gtggcgacaa tgccgtccat cgccacgcgg
    15361 acgtcgtcgg gattgtgccg ccacgccacg ctccaccagc gattcgcgct ggcggcaagg
    15421 tcggcgaaca cctgcccggc caaccggcgc agcaggtggc ccttgtcctc gaagtagacg
    15481 tagaagctgg cacgcgaaat gccggcctcg gttgacagcc ggtccacgct gagctcggtg
    15541 aaactggcgc catcgcgcat cagccgctcg gcggcgtcca gcagccggcg ctccatctgc
    15601 tctcgccgct gctggcgtcg ctgttcgcga ttggcctgcg gtttccgcgt caccgacggc
    15661 atttgccgag catagcgaac ctttggacac attgactaga catggcgtct aggcatattg
    15721 tcggtcgaga ttgtgacagg tgtcataagt gacggaggat ctgcaatgtc tgtgatgacc
    15781 gggacgtccg gcaccgaaaa gccgggccgt gcctacgacg cgatcgatct gtcgtcgcgt
    15841 gcgttctggt ccacgaccgc ggccgatcgc gaacgatcat ttgcggtgct gcgggccgag
    15901 cggccggtga gctggcatcc tcccgtcgag gacgccctga tgcacgatcc cgatgacccc
    15961 ggctattggg cgatcacccg acgctccgac atcgtcgcgg tcagccgcaa caacgaggtg
    16021 ttcctgtctg gccaaggggt gcttttcgag aatatcccgg tggagttgct cgaagcgtcg
    16081 cagtccttcc tggcgatgga cccgcctcgg cataccaagc tccgcaaagt cgtcagtgcc
    16141 gcattcaccc cgcgccaggt gcggcgtatc gaggactcga tcaaatcgaa tgccaagacc
    16201 atcgtcgagg agttgcgcgc ggccggcagc ggtgccgatt tcgtcgaccg ctgtgccaag
    16261 gaagtgccca tccgcaccct gtcggacatg gtcgggattc cggaatccga gcgagaacgc
    16321 atggcgcacg ccaccgacgc gctggtgtcc tgggccgacc cggtctactt ggatgggcgt
    16381 aacccgctgg aggttctgct cgaaaaccag atgtacctgc accaagtcgc cggcatgctg
    16441 gccgcgcagc gccgtgagca tcccggcgac gacctgctca gcagtctggt caacgccgag
    16501 gtggacggcg atcggctgac cgatgcggac gtggccgcgt tcttcgtgtt gctttcggtc
    16561 gccggcaacg acaccacccg ccagaccatc agccatgcca tgaaggcgct caccgacttt
    16621 cccggtgaga gggcctggct gctagaggat ttcgacaatc ggatcggcac ggcggtcgag
    16681 gagttcatcc gttgggcaac gcccgtcatg accttccgtc gcacggccgc aacggattcc
    16741 gagttaggtg ggcagaggat ccgggcgggg gagaaggtgg tgatgttcta tccgtccggt
    16801 aactgggacc gcgaggcgtt cgaccatccg gaccgtttcg acctgagtcg cagtcccaac
    16861 ccgcacctgg gcctcggcgg tggcggagtg cacttctgtc tcggagctag tgtgggccgg
    16921 gcgcagttgc gtgccatctt cggcgaactg ctgacgcagt tgcccgacat ccaggcgggc
    16981 gatccggcct acgtgccggg taacttcatc cacgccatcc gcagcttgcc ctgcaccttt
    17041 tagcgcgtcg ccgccgctgg cgcccgtgcc ggtgccccca ccaattccga ggcgagcagc
    17101 gccagcaatt cggagcaccc gctgtcgacc ttgacggcgg ccaggttgtc gccgcgcgtg
    17161 tggccgcggt tgacgatcgc gaccgggatg cccagcgcgg ccgcgcggcg cacgaatcgg
    17221 tagccggaga acacggtcag cgacgagccc gcgaccagca gcgcttcggc ctggtcgacc
    17281 agtgaatagg cttgggccac cacggctttg gacacgctct cgccgaagta gacgatgtcc
    17341 ggcttgagca tgccgtcaca gcgcgggcag tcgagatagc ggaacgacgc ggtatcggcg
    17401 acaacggcgt cggcgtcggg agccaccgcc agcccgccga ccgcttcggc tcgctcgatg
    17461 aatcccgggt tgagcgcctc gagccgttcg gccagtgtgg ctcggctcat gccgtagctg
    17521 cagcccaggc agatcacccg cgcgtaggtg ccgtgcagat tgacgacgtt gaggctgccc
    17581 gctttggtgt gcagcagatc gacgttctgg gtgatcacgc cggtgaccac cgaagcgcgc
    17641 tcgagcgcgg ccaatgcccg gtgcgccgcg ttgggcaggg tgtcgtgcat gtggcgccac
    17701 ccgacgtggt tgcgcgccca ataccgttgc cggaacacgg gatccgaggt gaactggcgg
    17761 atcgtcatgg gattgctcgg cggcgaatcg ggtccccggt agtcgggaat gccggagtcg
    17821 gtggatatcc ccgcgccggt caccgccgcg acccggcgac cggccagcag tgcgacgagc
    17881 tccggtgatt ctgcgcaacg ggcggtcacc tactcgagga taatcggctt cagccagcga
    17941 gcccggt
//
LOCUS       DASSGQ010000072        13514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_430490,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000072 DASSGQ010000000
VERSION     DASSGQ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13514
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430490"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..86)
                     /locus_tag="VFI55_03195"
     CDS             complement(<1..86)
                     /locus_tag="VFI55_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HET7073072.1"
                     /translation="MKTHITCPCGEAIVGKDEDDLVELTQAHL"
     gene            complement(119..694)
                     /locus_tag="VFI55_03200"
     CDS             complement(119..694)
                     /locus_tag="VFI55_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HET7073073.1"
                     /translation="MPNLQLVQDPDADALLDDNPFALLVGMLLDQQVPMETAFAGPKK
                     IADRMGGFDAAEIADHDPDKFAALCSERPAIHRFPGSMAKRIQALAQIIVDRYDGDAA
                     GLWTAGQPDGQELLRRLKGLPGFGEQKAQIFLALLGKQYGVTPAGWQAAAGEFGKPGT
                     CMSVADIVDAQSLGKVRSYKKQRKAAAKAAE"
     gene            complement(788..964)
                     /locus_tag="VFI55_03205"
     CDS             complement(788..964)
                     /locus_tag="VFI55_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5302 domain-containing protein"
                     /protein_id="HET7073074.1"
                     /translation="MAESNSHEEDETKRKFREALDRKKAKSAGASAHKDGGDKQSRTH
                     GPVENRREFRRKSG"
     gene            complement(1006..1449)
                     /locus_tag="VFI55_03210"
     CDS             complement(1006..1449)
                     /locus_tag="VFI55_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET7073075.1"
                     /translation="MGRQVFDDKLLAVISGNSLGVLATIKRDGRPQLSNVSYHFDPRE
                     QLVQVSITEPRAKTRNLRRDPRASILVAADDGWSYAVAEGAAELTPPAAAPDDDTVEA
                     LIALYRSIAGEHPDWDEYRQAMVTDRRVLLTLRISHVYGMPPGKR"
     gene            1525..2046
                     /locus_tag="VFI55_03215"
     CDS             1525..2046
                     /locus_tag="VFI55_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET7073076.1"
                     /translation="MTKYAAFLRGVNVGGVNLKMAEVSTALTDAGFENVRTILASGNV
                     LLESSAGVAAVRKKAEGALRERFGYDAWVLAYTIDRVRAIVDAYPFEREVDGYQSYVT
                     FVAEKAVLTELTALVESAGANEKIAPGEGVVYWQVPKGSTLDSTIGKTMGKPRYKSST
                     TTRNLRTLAKVLA"
     gene            complement(2058..2423)
                     /locus_tag="VFI55_03220"
     CDS             complement(2058..2423)
                     /locus_tag="VFI55_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7073077.1"
                     /translation="MELRDLLRVDTKARKPLFDQLRNQVIDGVRTGALQPGARLPTVR
                     DLAGQLGVAANTVGRAYRELESAAIVETRGRYGTFISRFDPTDAAMAAAAKEYVQVAR
                     SLGLTKSDAIRYVTNVPDD"
     gene            2461..3177
                     /locus_tag="VFI55_03225"
     CDS             2461..3177
                     /locus_tag="VFI55_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HET7073078.1"
                     /translation="MRVAVLSGAGISAESGVPTFRDDKNGLWARFDPYELSSTQGWRE
                     NPERVWGWYLWRHYLVAKVEPNPGHRAIAAWQDHVEVSVITQNVDDLHERAGSAPVHH
                     LHGSLFEFRCERCDLPYTNALPEMTEPTLEVEPPLCPRCGAMIRPDIVWFGEPLPDEP
                     WQRAVEATEAADVMVVVGTSAIVYPAAGLPDLALSRGTAVIEVNPEVTPLTSSATISV
                     RETASQALPGLLQRLPALLR"
     gene            3310..4161
                     /locus_tag="VFI55_03230"
     CDS             3310..4161
                     /locus_tag="VFI55_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073079.1"
                     /translation="MTSNELFIGSEAVQSGTLRKHQLRSRFRRVLPDVYAPRDHRLTL
                     RDRTMAAWLWSQRRGVLASLTAAAWHGSKWVDERLPIELVWSNARPPRGVTYGMALLP
                     AEVQMVAGVRVTTPRRTAFDIGRRQATGTAIAHLDALMRATGTKLGEVLEVADHHRGA
                     RGLRQLETALDLVDPGAQSPKETWLRLLLIRAGLPRPTTQIPVMTARAAQVYHLDMGW
                     EDLVVAVEYDGEHHRLDRWQYTRDIHRREALDRLGWIVIRVIASDRPADVIGRVRDAL
                     QFRASSL"
     gene            complement(4253..4894)
                     /locus_tag="VFI55_03235"
     CDS             complement(4253..4894)
                     /locus_tag="VFI55_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7073080.1"
                     /translation="MTQLAVSKIDNPFFAHVWPVVVTHETQAVRALRRENVSGLSGRV
                     LEVGAGVGTNFANYPQTVEEVIAVEPEPRLAARARAAANAAPVRVVVVGESAEGFSDA
                     QPFDAVVCSLVLCSVRDPGGVLHRLYTFLRPGGQLRYLEHVASAGGRGRLQRLADATL
                     WPRLFGNCHTHRDTERSIVDAGFEIDTSRREWTLPIWAPLPVSELVLGRARRP"
     gene            complement(4891..5586)
                     /locus_tag="VFI55_03240"
     CDS             complement(4891..5586)
                     /locus_tag="VFI55_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7073081.1"
                     /translation="MEGLRRVRAPRGSGDRLRDEILDAATELLLETGHVRSVSIRSVA
                     QRVGVTPPSIYLHFADKDALMDAVCARYFEKLDLEMQGVASGRSSAVEVLRAQGLAYV
                     RFATQTPELYRIATMGEWRSGSNIDTTLDSSAFKHMRATVQKLMDEGIYLPDDPTVVA
                     LELWSAAHGVAAMLIAKPHLPFGDAEAFADRVLYAVFCGRVVIGHCGTDVTPQKLATW
                     VTARRPAQEEPRA"
     gene            complement(5594..7927)
                     /locus_tag="VFI55_03245"
     CDS             complement(5594..7927)
                     /locus_tag="VFI55_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET7073082.1"
                     /translation="MLQAIARLAIAAPRRIIAAALLVFVAAAVFGIPVAKSLSPGGFQ
                     DPNSESAHAINVLADKFGQSGQQMLILLTAPAGANSEQARKVGTDLVGQLQRSPLVLN
                     VSSAWTAPPQAAADLVSTDGKSGLIVVNLKGGENFVQKNAQTLSDQLVHDRDGVTVIA
                     GGPAMQYAQINKQNQDDLLVMEIIALPMSFLVLIWVFGGLLAAALPMALGALAVVGSM
                     SVLRLVTFTTEVSIFALNLSTALGLALAIDYTLLIVSRYRDELAEGSDRDEALIRTMA
                     TSGRTVLFSAVTVALSMSATALFPMYFLKSFAYAGVATVAFVAISSIVITPAAIVLLG
                     PRLDALDVRRLLRRVFRRPDPAHKPVEELFWYRSSRFVMRRWAPIGLAVMALLLLLGL
                     PFFSVKWGFPDDRVLPRSASSHEVGDRLRSGFAHDSATAVPVVIPDARGLSPADLDKY
                     AVDLSRIPDVSAVSAPNGTFVNGNLVGPPAAATGLADGSAFLTVSSTAPLFSQSSDTQ
                     LRRLHQVAGPAGRSVEMAGVAQVNRDSVDAVTDRLPLVLGLMAAITFVLLFLLTGSVV
                     LPAKALICNILSLTAAFGALVWIFQDGHLGALGTTPSGTLVANMPVLLFCIAFGLSMD
                     YEVFLISRIREYWLASGAARPAKVSAKQAHAANDESVARGVARTGRVITAAALVMSMS
                     FAALIAAHVSFMRMFGLGLTLAVIADATLVRMVLVPAFMHVMGRWNWWAPEPLAWLHQ
                     RFGISETPAQDIGYGEPTDAPTQHNGRPIRESVTNIG"
     gene            8217..8348
                     /locus_tag="VFI55_03250"
     CDS             8217..8348
                     /locus_tag="VFI55_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073083.1"
                     /translation="MWIDDSSADVIKADFEALYHGDMLVEGETSEQFEEWHPLPTAS"
     gene            complement(8497..9258)
                     /locus_tag="VFI55_03255"
     CDS             complement(8497..9258)
                     /locus_tag="VFI55_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HET7073084.1"
                     /translation="MEIRDAVAVVTGGASGLGLATTKRLLDAGAQVVVLDLRGEDVVR
                     ELGDRARFAPADVTDEAAVTSALDTAESLGALRILVNCAGTGNAIRVLSRDGVFPLAA
                     FRKIVDINLIGTFNVLRLAAERIAKTEPIGPNGEERGVIVNTASVAAFDGQIGQAAYS
                     ASKGGVVGLTLPVARDLAGHLIRVVTIAPGLFDTPLLASLPEEARASLGKQVPHPSRL
                     GNPDEYGALAVHIIENPMLNGEVIRLDGAIRMAPR"
     gene            complement(9301..10392)
                     /locus_tag="VFI55_03260"
     CDS             complement(9301..10392)
                     /locus_tag="VFI55_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HET7073085.1"
                     /translation="MNGPLKGLRVVELAGIGPGPHAAMILGDLGADVVRIDRPSAGGV
                     GSGGAAKDAMLRNRRIVTADLKSDDGRGLVLKLVTRADVLIEGYRPGVTERLGLGPED
                     CAKVNERLVYARMTGWGQTGPRSQQAGHDINYISLNGILHAIGRVNERPVPPLNLVGD
                     FGGGSMFLLVGILAALWERQTSGKGQVVDAAMVDGSSVLIQMMWQMRTSGMWTDVRGA
                     NLLDGGAPYYDTYECADGRYVAVGAIEPQFYAAMLTGLGLDAANLPAQNDVARWPELR
                     GVLTDAFASRDRDHWAKVFANSDACVTPVLAFGEVHTEPHITERNTFYEVNGGLQPQP
                     APRFSRTVPDTPRPAAPATDVEALLEEWV"
     gene            10515..11071
                     /locus_tag="VFI55_03265"
                     /pseudo
     CDS             10515..11071
                     /locus_tag="VFI55_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
     gene            11157..12515
                     /locus_tag="VFI55_03270"
     CDS             11157..12515
                     /locus_tag="VFI55_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HET7073086.1"
                     /translation="MVERARCLVTGATGYIGARLVPRLLDEGHRVRGLARDPSKLAEV
                     PWREHADVVRGDVADVDSLITAFDGIDVIYYLVHSMGTSKDFAAAEARAVRNVVTAAR
                     RTGVRRVVYLSGLHPETGKLSDHLESRKAVGAALIDSGVEAVVLQAGVVIGTGSASFE
                     LIRHLTDRLPVMTTPKWVRNRIQPIAVRDVLHYLVAAATAPVPSSRTWDIGGPDVLEY
                     ADMMRVYAQVAGLSRRYLIVLPFLTPTIASLWVGTVTPIPPGLARPLIESLECDAVMS
                     DFDIDSIIDPPPGGLIGYRRAVELALDRATRGLPDATWASLRSEPAAPLPSDPDWAGA
                     TVYTDARNVRTSTEPGELWKAAEAATCGGRLGRGRLPGRWTVAEREPGSRLRLHGHLR
                     ALGRAWLEITITPHDGGSAYTQRAIFFPSGVVGRLCWFAVWPLRRAALGALARDVVTP
                     GR"
     gene            complement(12522..13244)
                     /locus_tag="VFI55_03275"
     CDS             complement(12522..13244)
                     /locus_tag="VFI55_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET7073087.1"
                     /translation="MSQSTTPSHATVLSELRRAMTNVAVPHNEPPGVVRRRRVVVAIT
                     LVIGAAVLGFSLRRHPGESSFYWLTLALAAVWIVGGFASGPLHLGGICWRGRNQRPVI
                     TGTTVGLLLGGVFVLGGLIVKEIPAFSALITRVLLFAHQGAFPLIVAITVVNGVAEEI
                     FFRGALYSALGRYHPVVISTILYACVTMASGNAMLGFAAIVLGTVCALERRASGGVLA
                     PVLTHFVWGVIMVLALPPMFGV"
     gene            complement(13241..13393)
                     /locus_tag="VFI55_03280"
     CDS             complement(13241..13393)
                     /locus_tag="VFI55_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073088.1"
                     /translation="MRILITGATGYVGSRLVTGDCTATDPGTLAGALRTGLDMPFTVT
                     PKACSA"
BASE COUNT         2330 a         4568 c         4431 g         2185 t
ORIGIN      
        1 aggtgcgctt gggtcagctc gaccaggtcg tcctcgtcct tgcccacgat ggcttcgccg
       61 cacggacacg ttatgtgtgt cttcacgctg ccgccctttc tccttagcca ctcagcagct
      121 actcggccgc tttggccgcc gctttccgct gctttttgta tgagcgcacc ttcccaaggg
      181 actgggcgtc gacgatgtcg gcgacggaca tgcatgtgcc gggcttgccg aactccccgg
      241 ccgccgcctg ccagcccgcc ggcgtcacgc cgtactgctt gccgagcagt gccagaaaga
      301 tctgcgcctt ctgctcgccg aagccgggta accccttgag ccggcgcagc agttcctgcc
      361 cgtcgggctg gccagccgtc cacaggccgg cggcatcgcc gtcgtagcgg tccacgatga
      421 tctgcgccag ggcctggata cgtttggcca tcgaccccgg aaagcggtgt atcgcaggcc
      481 tttccgagca caatgcggcg aacttgtccg ggtcgtgatc ggcgatctcg gcggcgtcga
      541 atccgcccat ccgatccgcg atcttcttcg gtcccgcgaa ggcggtttcc atgggcacct
      601 gctggtctag cagcatgccg accagcaacg cgaacgggtt gtcatccagc agtgcgtcgg
      661 cgtccggatc ttggacaagc tgcaaattcg gcacccccac atagtaaaac tgcgccgaaa
      721 tcccgacact ggcggccgaa gtcgacggca accgcgacga acacgttgac ggccagtgaa
      781 ctatggccta gccgctcttg cggcggaatt cccgacgatt ctcgaccgga ccgtgcgtac
      841 gggattgctt gtcgccgccg tctttatgag cggacgcgcc cgccgatttg gccttcttgc
      901 ggtcgagggc ttcacggaac ttgcgcttgg tctcgtcctc ttcgtgcgag ttcgattcag
      961 ccataccggc agcctagcgt cgacaggccc ggcctggctt gtgatttacc gtttgcccgg
     1021 cggcatgccg tacacgtgcg agatccgcag cgtcagcagc acccgccggt cggtgaccat
     1081 cgcctgccgg tactcgtccc agtccggatg ctcaccggcg atactgcgat acaaggcaat
     1141 cagcgcctcg acggtgtcgt cgtcgggcgc ggccgccggc ggcgtcagtt ccgcggcgcc
     1201 ctcagcgacg gcatacgacc atccatcatc ggcggccacc aggatcgatg cccgcgggtc
     1261 gcggcgcagg ttgcgcgtct tggcccgcgg ctcggtgatc gagacctgca ccagttgctc
     1321 gcgcgggtcg aagtgataac tcacgttcga cagttggggc cgtccgtcac gcttgatggt
     1381 ggccagcacc ccgagtgagt tcccactgat cacggccagc aacttgtcgt cgaagacttg
     1441 gcgtcccatg tcgaaagcct acgtcgctgc gccgaacgcc ggtccgaatg tcgccgtcgc
     1501 agtgtgggct ggtggaatcg gttcatgacc aagtacgcag cgttcctgcg cggcgtcaac
     1561 gtcggcggcg tcaacctcaa gatggccgag gtgtccaccg ctctgaccga cgcgggattt
     1621 gagaatgtgc gcaccatcct ggccagcggc aacgtgctgt tggagtcgtc ggcgggcgtc
     1681 gccgcggtgc gcaagaaggc cgaaggcgcc ttgcgcgagc ggttcggtta tgacgcgtgg
     1741 gtgctggcct acaccatcga tcgggtgcgc gcgatcgtcg acgcctaccc cttcgagcgc
     1801 gaggtcgacg ggtatcagtc ctacgtcacc ttcgtcgccg agaaagcggt gctcaccgaa
     1861 ctcaccgccc tggtcgagag tgccggcgcg aacgagaaga tcgcccccgg tgaaggcgtc
     1921 gtctactggc aggtacccaa gggcagcacc ctggacagca cgatcggcaa gacgatgggc
     1981 aagccgcgct acaagtcctc gacgacgaca cgcaacctgc gcacgctggc caaagtgttg
     2041 gcgtgaatgc gcgcgcctca gtcgtcgggc acgttggtca cgtagcggat cgcgtcggac
     2101 ttggtcagcc ccaacgatcg cgccacttgg acatactcct tggcggcggc ggccatcgcg
     2161 gcatcggtcg gatcgaagcg ggaaatgaac gtgccatacc gcccccgcgt ttcgacgatc
     2221 gccgcggact ccagctcccg gtacgcacgg cccacggtgt ttgccgcaac gcctagctga
     2281 ccggccagat cgcgcaccgt cgggagccgg gcaccgggct gcagcgcgcc cgtccggact
     2341 ccgtcgatga cctggtttct gagttggtcg aacaacggtt tgcgtgcctt ggtgtcgacc
     2401 cgtaacaaat cccgtagctc cacgtgacca gtatcacgca accgcggcta tgtttgtggg
     2461 atgcgtgtgg cggtgctcag tggcgcgggg atctccgctg agagcggcgt gccgacattc
     2521 cgggacgaca agaacgggct gtgggcccgc ttcgatccct acgagctgtc cagcacacag
     2581 gggtggcgcg agaaccccga gcgggtgtgg ggatggtatc tgtggcgcca ttacctggtg
     2641 gccaaggttg agcccaaccc cggacatcgc gcaatcgccg cctggcagga ccacgtcgag
     2701 gtcagcgtca tcacgcagaa cgtcgacgac ctgcacgagc gggcgggcag cgcgccggtg
     2761 caccacctgc acggaagtct tttcgaattc cgttgtgagc gttgcgattt gccctacacc
     2821 aacgcgttgc ccgagatgac cgaaccgacg ctggaagtgg aacctccgct ctgcccccgc
     2881 tgcggcgcga tgatcaggcc ggacatcgtg tggttcggtg agccgctgcc cgacgagccc
     2941 tggcagcgtg cggtcgaggc gaccgaggcc gccgacgtga tggtggtggt gggaacctcg
     3001 gcgatcgtct atcccgcggc cggcctgccc gatctggcgc tgtcacgcgg cacggccgtg
     3061 atcgaggtca atcccgaggt gacaccgctg accagcagcg ccacgatcag cgtgcgcgag
     3121 accgcgagcc aggctttgcc cggtctgctg cagcgactgc ccgccctgct gaggtagccc
     3181 cacattcgcc ggcgccgagt ctgcggcagc ggcgtcgagc ctgcacacag atcgcggtcc
     3241 gcccacaaaa tcgcgcgccg agcgcagatt cgctgcgacc gagccggcaa cgtcggcaag
     3301 tctgccgtca tgacgagcaa cgaactcttc atcggcagtg aggctgtgca gagcggcacg
     3361 ctgcgaaagc atcagctgcg ttcccgattc cgccgggtgc ttcccgatgt ctatgcccca
     3421 cgcgatcacc ggctcacact gcgcgaccgc acgatggccg cctggctgtg gtcacaacgc
     3481 cggggagttc ttgcgtcgtt gaccgcggct gcgtggcatg gctcgaaatg ggtggacgaa
     3541 cgcctgccca tcgaattggt ctggtcgaac gcccggccgc ctcgtggtgt gacctatggc
     3601 atggcgctgc tgccagcgga agtccagatg gtcgccgggg ttcgggttac gacgccgcga
     3661 cgtaccgcat tcgacatcgg tcgtcgtcag gcgacgggga cggcaattgc gcatctagac
     3721 gccctcatgc gagcgaccgg caccaagcta ggtgaagtcc tcgaagtagc cgaccaccat
     3781 cgcggcgctc gagggcttcg gcagctggaa acagcgctgg acttggtcga tccgggtgca
     3841 cagtcgccga aggagacgtg gctgcggctc ctattgatcc gcgcaggctt accgcgaccg
     3901 acgacccaga tcccggtgat gactgccagg gcagcgcagg tgtatcacct cgacatgggg
     3961 tgggaggacc tcgtggtcgc cgtcgagtat gacggcgagc atcatcgtct ggaccgctgg
     4021 cagtacacca gggacatcca ccggcgcgag gccttagacc ggctgggctg gattgtcatc
     4081 cgggtgatcg cgtcagatcg cccggccgac gtcattggca gagttcgcga tgcgctgcag
     4141 ttccgggcat cgagcctgta gtggcggcgc cgagcctgcg cacagatcgc ggtttactcg
     4201 ggaacgcacg cgctcagcgc agtctcggcg tagatggaag gcggaaacag tgtcagggcc
     4261 tgcgcgcgcg gcccagcacc aattccgaca ccggcaacgg ggcccagatc ggcagcgtcc
     4321 actcgcggcg cgacgtgtcg atctcgaatc cggcatcgac aatcgaacgc tctgtatcgc
     4381 gatgggtgtg gcagttgccg aataacctcg gccacagcgt cgcgtcggcc aaccgctgta
     4441 gtcggccccg tccgcccgcg ctggccacgt gctcgagata ccgcagctgg ccgccgggcc
     4501 gcaagaacgt atacagacgg tgcagcaccc cgccgggatc gcgcaccgag cacaacacca
     4561 gcgagcacac gaccgcgtcg aacggctgcg cgtcgctgaa cccctccgcg gactccccga
     4621 cgacgacaac ccggacgggc gccgcgttgg ctgcggcccg ggcgcgcgcg gccagccgcg
     4681 gctcgggctc cacggcgatc acctcttcga ccgtttgcgg gtagttggcg aagttcgtcc
     4741 cgacacccgc gccgacttcg aggacccggc ccgagaggcc ggacacattc tcccggcgaa
     4801 gcgcgcgcac cgcctgggtt tcatgtgtga cgacgacggg ccagacatgc gcgaagaacg
     4861 gattgtcgat cttggacacc gctagctgcg tcatgcccgc ggctcctcct gcgccggccg
     4921 gcgcgcggtc acccaggtcg ccaacttctg gggtgtgaca tccgtgccgc aatgcccgat
     4981 caccacccgc ccgcagaaga ccgcgtacag tacccggtcg gcgaacgcct cggcatcgcc
     5041 aaaaggcaag tgcggcttgg cgatcaacat agcggccact ccgtgcgcgg cgctccagag
     5101 ttccagggcg acgacggtcg ggtcgtccgg caggtaaatg ccttcgtcca tcagcttttg
     5161 cacggtggcc cgcatgtgct tgaacgccga gctgtccagc gtcgtgtcga tgttgcttcc
     5221 ggagcgccac tcgcccatcg tggcgatccg gtacaactcc ggggtttggg tggcgaaccg
     5281 gacgtaggcc agcccttggg cgcgcagcac ctcgaccgcc gagctgcgcc cgctggcgac
     5341 cccctgcatt tccaggtcga gcttttcgaa gtagcgcgcg caaacggcgt ccatcagcgc
     5401 gtctttgtcc gcgaaatgca ggtagatcga cgggggtgtg acgccgacac gttgtgccac
     5461 ggaccgaatc gacaccgacc tgacgtgccc ggtttccagc agcagctcgg tggccgcgtc
     5521 cagaatctcg tcgcggagcc gatcgcccga cccgcggggg gccctgacgc gccggagccc
     5581 ttcgatcgtg acgttaccca atgttggtca ccgactccct gatcgggcgc ccgttgtgct
     5641 gggtcggtgc gtcggtgggc tcgccgtatc caatgtcctg ggccggtgtc tcgctgatgc
     5701 cgaaccgctg atgcagccag gccaacggct ctggcgccca ccaattccac cggcccatca
     5761 cgtgcatgaa cgccgggacc aggaccatcc gcaccagcgt cgcatcggcg atcacggcaa
     5821 gcgtcaggcc gaggccgaac attcgcatga acgacacgtg ggcggcgatc aacgcagcga
     5881 acgacatcga catcaccaac gcggcggcgg tgatcacccg ccccgtgcgg gccacgccgc
     5941 gtgccacact ctcgtcattc gcggcgtgcg cctgcttagc gcttaccttt gccggccgcg
     6001 cggcacccga ggccagccag tactcgcgga tccgggaaat caggaacacc tcgtaatcca
     6061 tggacaagcc gaacgcgatg cagaacaaca gcaccggcat gttcgccacc aacgtgccac
     6121 tcggagttgt tccgagcgca ccgagatgac cgtcctggaa aatccacacc agcgcgccga
     6181 acgcagcggt caaggacagg atgttacaaa tcagcgcctt ggccggcagc accacgctgc
     6241 ccgtgagcaa gaacagcaac acgaacgtga tggccgccat cagccccagc accagcggaa
     6301 gccggtccgt caccgcgtcg acgctgtcgc ggttgacctg cgcgacaccg gccatctcga
     6361 cggatcggcc ggcggggccg gccacctggt gtaaccgtct gagctgggtg tcagaagatt
     6421 gcgagaacag cggcgccgtg ctgctcacgg tcaggaatgc actgccgtcg gccaacccgg
     6481 tcgcggccgc cggtggcccc accaggtttc cgttgacgaa cgtcccgttg ggggcggaca
     6541 ccgccgacac atcgggaatc cgtgacaggt cgacggcgta cttgtcgaga tccgccgggc
     6601 tcaggccgcg ggcgtcgggg atgacgacgg gcaccgcggt tgccgaatcg tgcgcgaaac
     6661 cactgcgcaa tcggtcaccg acctcatgcg acgacgccga acgcggcagc acccggtcgt
     6721 cggggaaacc ccacttcacc gagaagaacg gaagcccgag cagcaacagc agcgccatca
     6781 ccgccagccc gatcggcgcc cagcggcgca tcacgaacct actcgaccgg taccagaaca
     6841 gctcctcgac gggtttgtgt gccgggtcgg gccggcggaa gacccggcgc aacagccggc
     6901 ggacgtccag cgcgtccagc cgggggccca gcagcactat cgcggcgggc gttatcacga
     6961 tggacgagat cgcgacgaag gccacggtgg ccaccccggc gtaggcgaac gatttcagga
     7021 agtacatcgg gaacagcgcc gtcgccgaca tcgacaacgc cacggtgacc gcggagaaca
     7081 gcaccgtgcg gccggaggtg gccatggtcc ggatcagtgc ctcgtcccgg tcgctgccct
     7141 cggccaactc gtcgcgatac ctgctgacga tcaacagcgt gtagtcgatg gccagggcca
     7201 agcccagcgc ggtgctcagg ttgagcgcga agatcgacac ttcggtggta aaggtgacga
     7261 gccgcaacac cgacatggag ccgacgacgg ccaatgcgcc gagggccatt ggcaacgccg
     7321 ccgccagcag cccaccgaac acccagatca gcaccaggaa gctcatcggg agcgcgatga
     7381 tttccatcac cagcaggtcg tcctggttct gcttgttgat ctgggcgtac tgcatcgccg
     7441 ggccgccggc gatgacggtg acgccgtcac gatcgtggac gagttggtcc gacagagttt
     7501 gggcgttttt ctgcacgaag ttttcgccgc ctttgaggtt gaccacgatc aaccccgact
     7561 tgccgtcggt gctgaccaga tcggcggcgg cctgcggcgg tgcggtccac gcggacgaga
     7621 cattgagtac cagcggggac cgttgcaatt ggccgacgag gtcggtgccc accttgcggg
     7681 cctgctcact gttggcgcct gcgggtgccg tcagtaagat cagcatctgc tgaccgctct
     7741 gcccgaactt gtcggccaac acattgatgg cgtgggccga ttccgagttc ggatcttgga
     7801 aacccccggg agacaggctc ttagcgacag gaatgccaaa caccgctgcg gctacgaaga
     7861 ccaagagggc cgccgcgatt attcgtcgcg gcgccgcgat agccagccga gcgatcgctt
     7921 gcagcatgtt tccggtcctt ccaccgcgag cggcggtcgt gccgacccgc cacctatccg
     7981 cggtaactta tcaacgataa gttacacgtg tcaaacagca tcccgcccag tactacggac
     8041 gagggcaccc gatggttcac ccgccttccg aagcgtcatg ccggccagcg cgaaagcggt
     8101 caccacatgc gcctgatgct tcattaccca cgagaaacct acccgccggt aaattgccat
     8161 tgttttccga atccaggctc aaagattcct tagagctgac ccataggctc atggtcatgt
     8221 ggatcgacga ctcgagtgcc gacgtcatca aagctgactt cgaggccctc taccacggcg
     8281 acatgctggt ggaaggtgag acctccgagc agttcgaaga gtggcacccg ctacctacgg
     8341 caagctgacc ggccatgagc gtgctcgcgc ggctgcttat ggccgaaccc tcgatcactc
     8401 gctggtttcg tcgatagatc tcgttcctca atgtcagccc cccgcaatgc ggggggcttt
     8461 cgtctgtccg ctagtccttc cctgcgggag gcggccttac cgaggtgcca tgcggatggc
     8521 gccgtccaga cggatgacct ctccgttgag catcggattt tcgatgatgt gcaccgcgag
     8581 cgccccgtat tcgtccgggt tgcccagccg tgacgggtgc ggcacctgct tacccagcga
     8641 tgccctggcc tcctcgggca aagaggccaa cagcggcgtg tcgaagagcc cgggcgcgat
     8701 ggtgaccacc cggatgaggt ggccggccag gtcacgcgcg accggcaggg tcaggccaac
     8761 cacgccgccc ttggacgccg agtaggcggc ctggccgatc tggccatcga acgccgcgac
     8821 cgacgcggtg ttgacgatga ccccgcgctc ctccccgttc gggccgatgg gctcggtctt
     8881 cgcgatccgc tcggcggcga gtcgcagcac gttgaaggtt ccgatcaggt tgatgtcgac
     8941 gatcttgcgg aaggcagcca gcgggaaaac accgtctcgg ctcagcaccc ggatggcgtt
     9001 gccggtgccg gcgcagttga ccaggatgcg cagggcgccc agcgattcgg ccgtgtccag
     9061 cgcgctggtg acggcggcct cgtcggtgac gtcggcggga gcgaagcgcg cccgatcgcc
     9121 gagctcgcga accacgtcct cacccctgag gtccagcacc accacctgcg cccccgcgtc
     9181 cagcagccgc ttggtggtgg ccagacccag gcccgacgca cccccggtga cgacggctac
     9241 ggcgtctcta atctccatac gagcccttcc tcggttgccc agccaactag ttggttggga
     9301 ctatacccac tcttcgagca acgcctcgac gtcggtcgcc ggcgcggccg gtcggggtgt
     9361 gtctggcacg gtccgagaga accgcggagc gggctgcggc tgcaaaccac cgttcacttc
     9421 gtagaaggtg ttgcgctcgg tgatgtgcgg ctcggtgtgc acctcgccga acgccaacac
     9481 cggcgtcaca caggcgtcgg agttggcgaa cacctttgcc cagtggtcgc ggtcacgact
     9541 ggcgaacgcg tcggtcaaca ccccccgaag ttcgggccag cgggcgacgt cgttctgcgc
     9601 cggcaggttg gcggcgtcca gacccaggcc cgtgagcatg gccgcgtaga actggggttc
     9661 gatggcgccg accgcgacgt agcgtccgtc ggcgcactca taggtgtcgt aatagggcgc
     9721 gccgccgtcg agcagattgg cgccacgcac gtcggtccac ataccggaag tgcgcatctg
     9781 ccacatcatc tggatcagca cgctggaacc gtcgaccatc gcggcgtcaa cgacctggcc
     9841 tttgccggag gtttggcgct cccacagcgc ggccaggatg ccaaccagca agaacatcga
     9901 gccgccgccg aagtcgccga cgaggttgag tggcggcacc gggcgctcgt tgacgcggcc
     9961 gatcgcgtgc aggatcccgt tgagcgagat gtagttgatg tcatggcccg cctgctggct
    10021 gcgcggaccg gtctggcccc agccggtcat ccgggcatac accaggcgct cgttgacctt
    10081 cgcgcaatcc tcggggccaa ggccgagccg ctcggtgacg ccgggacggt agccctcgat
    10141 caacacgtcg gcccgcgtga cgagcttgag caccaggccc cgcccgtcgt cggacttgag
    10201 gtcggccgtg acgatgcgcc ggttgcgcag catggcgtcc ttcgccgcgc cgcctgagcc
    10261 gacgccacct gcggacggcc gatcgatgcg caccacgtcg gcccccagat ccccgaggat
    10321 catcgcggca tgtgggccgg gcccgatgcc ggccaactcg acaactctca gtcctttgag
    10381 aggtccattc agcggtccag ccacgaccca ccgacctttc gtctgctctg acgcgcccga
    10441 cccgtgcggg atcgaacaac cggttgtttg catcttcgca gccgccccac accccgctca
    10501 cctagggtgg gctcatgccg caatctgcga tcgacacgct ggcccccgtc gcaggtcttg
    10561 ccgtcacgct gtccgagggt gtcctctcgg tcaccatcga ccgccccgac agcctcaact
    10621 cggtgaccgc cccggtgctg gccggtatcg ccgacgcgat ggagttcgcg gccacggatc
    10681 cgcgggtcaa ggtggtgcgc ctcggcggat cgggtcgcgg cttttgctcc ggagcgggca
    10741 tgagcgccaa ggacgtggcc ggtggcggac cggggaccac gatcatcggg gaagccaacc
    10801 gggcggtgcg ggccatcacc gcgttgtcgc ggcccgtcat cgccgtggtt caggggccgg
    10861 cggccacaag gtggtttcca cgttgctcgc gggccccgcg ggggcgtacg aaaagaccaa
    10921 gcatgcgatc aacgctgcca cattgaccga attggatgcc gccctggagc gcgagcttcg
    10981 cggccaggcc gtcctcctgc gatcgcacga cttcatcgag ggcgcaacgg cattccagca
    11041 acgacgcaca ccccacttca ccgattgctg acggctcacc cgtttacttc cggcccgacc
    11101 ggcaatccct cgctgcagca gatccgaatt actgacaggc ggggaatgga ggggtgatgg
    11161 tggagcgggc ccgctgtctg gtaaccggag cgaccggcta catcggcgcc cggctggtgc
    11221 cgcggctgct ggacgagggc catcgagtcc gtggattggc ccgcgaccca agcaaattgg
    11281 ccgaagtgcc atggcgggag cacgccgacg tggtccgcgg cgacgttgcc gatgtcgatt
    11341 cgctgatcac ggcgttcgac ggcatcgacg tcatctacta cctggtgcac tcgatgggaa
    11401 cgtcgaagga tttcgccgcg gcggaagccc gcgccgtccg caacgtggtg acggccgccc
    11461 gtcgcaccgg ggtacgacgg gtggtgtatc tgagcgggct gcatcccgag accggcaagc
    11521 tctcggatca tctcgagtcg cgcaaggccg tcggtgcggc cctgatcgat tccggtgtcg
    11581 aggccgtggt gctgcaagcc ggggtggtca tcgggaccgg gtcggcgtcg ttcgaactga
    11641 tccggcacct caccgaccgg ttgcccgtga tgaccacacc gaagtgggtg cgcaaccgga
    11701 ttcagccgat cgcggtgcgc gacgtgctgc attatctggt cgccgcggca acggcaccgg
    11761 tgccgtcgtc gcgcacgtgg gatatcggcg gacccgacgt gttggagtac gccgacatga
    11821 tgcgggtcta cgcccaggtt gcgggcttga gcaggcgcta cctgattgtg ctgccgttcc
    11881 tgacaccaac gatcgcgagc ctgtgggtgg gcaccgtcac gccgattccg cccggactgg
    11941 cgcggccgct gatcgaatcg ctggaatgcg atgccgtgat gagtgacttc gacatcgaca
    12001 gcatcatcga tccaccaccg gggggcctga tcggctaccg ccgcgccgtc gaactggcgc
    12061 tggaccgcgc gacacgcgga ctccccgacg ccacctgggc ctccctgcga tcggagccgg
    12121 ccgcgccgct gcccagcgac ccggactggg ccggggcgac ggtatacacc gatgcgcgga
    12181 acgtccggac ctcgacggaa cccggcgagc tgtggaaagc ggccgaggcg gcgacttgcg
    12241 gcggccggct ggggcgaggc cgcttgcccg ggcgctggac cgtggcggag cgcgagcccg
    12301 gctcgcggct gcggctacat ggccatctgc gcgcgttggg gcgggcgtgg ctcgagatca
    12361 cgatcacccc gcacgacggc ggcagcgcgt acacgcagcg ggcgatcttt ttcccgagcg
    12421 gcgtcgtggg ccggttgtgc tggttcgcag tgtggccgct gcgcagggcc gcgctgggcg
    12481 ccctggcccg cgacgtcgtc accccgggac gctgaccgcc gtcaaacgcc gaacatcggc
    12541 ggcagcgcga gcaccatgat cacgccccac acgaagtggg tcagcaccgg agccagcaca
    12601 ccgccgctgg cccggcgctc cagcgcgcac accgtaccga ggacgatcgc ggcgaatccg
    12661 agcatcgcgt tgccgctggc catggtcacg caggcgtaca aaatggtcga aataaccacc
    12721 gggtggtacc ggcccaacgc gctgtacagc gcgccgcgga aaaagatttc ctcggcgacg
    12781 ccgttgacca cggtgatcgc aacgatcagc ggaaaagcgc cctggtgggc gaataacagc
    12841 acgcgggtaa tgagggccga gaaggccggt atctccttta cgatcagtcc gcccaacacg
    12901 aagacaccac caagcagcag gccgacggtc gttcccgtga tcaccggccg ctggttgcgg
    12961 ccgcgccagc agatgccacc cagatgcaaa gggcccgagg cgaatccacc gacgatccac
    13021 acggctgcca gtgccagcgt gagccaatag aaacttgatt cacccggatg gcggcgcagc
    13081 gaaaagccaa gcaccgcagc gccgatcacc agtgtgatcg cgacgacgac gcgccggcgt
    13141 cgcacgacac cgggcggttc gttgtggggc acagcgacat tggtcatggc gcgacgcaac
    13201 tcggataaca cggtggcgtg ggacggagtg gtcgactgac tcatgccgaa catgccttcg
    13261 gggtcactgt gaacggcatg tccagcccgg ttcgcagcgc accggccagc gtgccggggt
    13321 cagtggcagt gcaatcccca gtaaccagcc gcgatcccac ataaccggtg gcaccggtga
    13381 tgagaatccg catacccccg accgtaaccc ggcgacgatg cccgccgcgg cgcggcggga
    13441 gaaggagccg ggcaatcaga cctcagccgg gcgacgatgc ccgccgcggg acttgtccgg
    13501 tcgacgtcgt tacg
//
LOCUS       DASSGQ010000073         8065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_217712,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000073 DASSGQ010000000
VERSION     DASSGQ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217712"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..99
                     /locus_tag="VFI55_03285"
     CDS             <1..99
                     /locus_tag="VFI55_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7073089.1"
                     /translation="VLFLASDAARYITGQQLRVDGGGFLKVKPWGV"
     gene            201..1139
                     /locus_tag="VFI55_03290"
     CDS             201..1139
                     /locus_tag="VFI55_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET7073090.1"
                     /translation="MVLMKAADDRLSKGIGTGEFMCVYWPSRGQEAIAAAMGVALRPD
                     DQLVTTYRGLHDLIGKGVPLQEIYGEMMGRTVGASRGKGGTMHIANPDKGVMLSTGIV
                     GAGPPVAVGLAMAAKRKGLDRVTVVSFGDGATNTGSFHEAANMAALWDLPLVFVCQNN
                     LFAEMTPTSETMKLEHVAERASGYGMPGVRVDGNDPLAVKAVLDEALRRARRAGGPTF
                     IECVTFRFRGHYFGDRMPYIPKDQLAAAMAADPVPRFRDHLAEAGVCSLDELDRIDTD
                     ALATVEAALNTVMSAECPSIDELDRDVYANPIKFPV"
     gene            1161..2159
                     /locus_tag="VFI55_03295"
     CDS             1161..2159
                     /locus_tag="VFI55_03295"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET7073091.1"
                     /translation="MDEKEMTMREALNLALDQALGADDRVFLLGEDIADPGAAGPTAG
                     LSTKYGRERVLDTPISEAAILGAAVGAAIDGLLPVAEIMIMDFIGIAADQLINHAAKL
                     RFMTAGRTTAPITVRTQVYAGLATGATHSQSLEAWFMHIPGMKVIVPSTPRDGKGLLT
                     AAIFDEDPCLFIETIRLQSKKGPVPVQPGFSIPLGQADIKRHGSDVTLISYGRSVHDA
                     LAAAAALAEQAVSAEVVDLRTLVPLDVETIIESARRTRRVVIAHDAVQFCGPGAEIAA
                     ILQSELFGELAAPVERVGARFVPNPAAAALEAQVCPSPARIIAAAQRTLDKANVRG"
     gene            2152..2394
                     /locus_tag="VFI55_03300"
     CDS             2152..2394
                     /locus_tag="VFI55_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET7073092.1"
                     /translation="MADFIIRIPRISVAVSEAELTGLLVEAGQHVDEGTPIYVIATEK
                     AEQEIEAGASGTVQWTGHVGTTYDIGAEIGVITSSR"
     gene            2414..3286
                     /locus_tag="VFI55_03305"
     CDS             2414..3286
                     /locus_tag="VFI55_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET7073093.1"
                     /translation="MDLNETRERIIYEKEGPIARVTLNWPEKANAQDQKLAEEVDAAL
                     LDADRDYDIKVLILKANGKGFCSGHAIGNNAVDYPAFVEAAKVMGTPWKPQTDLFVKP
                     ILNLWEFSKPTIAAVHGYCVGGGTHYGLTTDIVIASEDAYFSYPPLQGFGMPSGECSI
                     EPWVFMNWRRAAYYLYLAEVIDAKRALEVGLVNEVVPREQLNDRVEAIARHIAQAPMT
                     TLLATKANLKRAWELMGMRVHWQSSNDLVALASISKDVQQLIQQVFKDKVLPSEQARR
                     QAAAAASTDGASAT"
     gene            3316..4860
                     /locus_tag="VFI55_03310"
     CDS             3316..4860
                     /locus_tag="VFI55_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HET7073094.1"
                     /translation="MNIADHATTAGQAPALIMPGGATITFGELYARSQRVAAVLHSAG
                     LRRGDGVALVLPNRPEFVEITWGCQLSGLYYTAVNTHFTPGEVAYVIDDSDAKAVFLD
                     ASDGSMAELAAHICDINAAVEVHIGVGGNLPDWLAYENALAPAGDAPPVSDGSEMLYS
                     SGTTGRPKAVRRPLPTDGNGSWAQAVLEMALVHKYGMNRSSVYLSPAPLYHAAGVNYT
                     MAVNRVGAASIIMGKFDAETVLRLIETHSVTHAQFVPTMFVRMLKLPDSVRLKYDVSS
                     LRCVIHAAAPCPVDVKHRMMKWFGPIIHEYYGGTEGFAGTTIGPTDWLAHPGSVGIPM
                     APVHVVGADGQELPVGQSGELYFEGGPDFEYFKDPAKTASVYNDRGWRSLGDVGYVDE
                     DGYMYLTDRSTFMIVSGGVNIYPQEVENLLVMHPKLVDAAVFGVPNHEFGEEVKAVVQ
                     PAAGVVSGPDLEAELIQYCRAQLAGYKCPRTVEFDPKLPRDPNGKLYKRRIRDRYWQG
                     RASRIV"
     gene            4882..6264
                     /locus_tag="VFI55_03315"
     CDS             4882..6264
                     /locus_tag="VFI55_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET7073095.1"
                     /translation="MAVETGAPRTEVNWTPLPVPWAVQSPNRIPKQRYYDAEFYALEA
                     EMFWPRVWQLACRLEEIPKPGDFVEYEILDESIIVVRVDTQTVRAYHNACRHRGVKLV
                     EGNGSRRAFVCPFHGWCWSIDGRNTFVARPEVFAEENLCPDDIQLVSVKCELWGGCAW
                     INLDDGAPALRDCIEPFASIYDAWKVESLRVEWWQSCRLPVNWKLATAAFMEGYHVPQ
                     THPQLLPPAQIGGPAAAVHPVIQNSLYFMRTLGDGMGGMTHESDIRIAEGLQHLELPT
                     DSTEAMTAWRSALNDAVVGWHRARGSTIPDLNDLLRRGITGAIGFCFPHHFILPTYSS
                     ASSYRIRPLGPEETLFEIWSLTRFPSDHSAGKPTPPEPMAPDDPRWPPIPAQDFSNLP
                     RQQKGLHSKGFEYMRLSNQIEGLISNFERVVDGFLAGLSYETLVPAVQKTNTTIDVPI
                     ADLGFTQEPR"
     gene            6261..7961
                     /locus_tag="VFI55_03320"
     CDS             6261..7961
                     /locus_tag="VFI55_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET7073096.1"
                     /translation="MTARWDRSVDLLIAGSGGGGMVAGLAALDCGLEPLIIEKQPLVG
                     GSTGLSGGIVWMPNNPLMRADGIADSHEDGLAYLADVVGDIGAPSSPARREMFLTAGY
                     DMINFLLRKDVRLIRCAGWSDYYPNHKGGNASGRAVEGTPFDAAELGSWRDKVQPPLA
                     KNYGYVVLTNELRSVQYFNRSPHAFTVATKVFLRTVAARMRRHQILTNGASLIGQMLK
                     MLIDLTDGQPPLWTNTAMDDLIVEDGRVVGARVTRDGATLDIEARRGVLLAAGGFGHN
                     ADMRRRYSGNQPNEAQWSIANAGDTGEVLQAAMRVGAQTDLLDEAWWLPSVFIANGGA
                     AAASLGSGRQRPGAIYVDSTGRRFCNESNSYVEVGKAMYANKAVPCWMIFDDGYVRRY
                     VASANPFKRNQHLPPELIDNGAVKRADTTAELARQIDLPAEELARTIQRFNQFAAKGL
                     DPDFGRGQSAYNDCLGDPGYRPNAAVGPLDRAPYYATRVFPADVGTCGGVITNEHAQV
                     LDEQDLVIEGLYATGNTTATVMGRTYPGAGASIASSMVFGYVAARHAAGRKIAGEMSR
                     "
BASE COUNT         1505 a         2630 c         2604 g         1326 t
ORIGIN      
        1 gtgttatttc tggcctccga cgcggcgcgt tacatcaccg gccagcaact gcgtgtcgat
       61 ggcggcggct tcctcaaagt caagccctgg ggcgtgtgac acatggtggc gccaatccgg
      121 ctgcggtgca acgaaaccgg aggcacgtgg tgagcgacgg tcggtccacg acagaggttg
      181 agcggcgcct gtacgaactg atggtcctga tgaaggcggc cgatgaccgg ctctccaagg
      241 gcatcggcac cggcgagttc atgtgcgtgt actggccgtc tcgtggtcag gaggccatcg
      301 cggctgcgat gggggtggcg ctccggcccg acgaccagct ggtgacgact taccgcgggc
      361 tgcacgacct catcggcaag ggggtaccgc ttcaggagat ttacggcgag atgatggggc
      421 gcactgtcgg cgcgagtcgc ggcaagggcg gcaccatgca tatcgccaat cccgataagg
      481 gtgtgatgct gtcgacggga atcgtcgggg cgggaccgcc ggtggccgtg gggttggcga
      541 tggccgccaa gcgcaagggc ctcgatcgcg tcaccgtcgt cagcttcggt gacggcgcga
      601 cgaataccgg ctcgttccac gaggccgcca acatggccgc cctgtgggat ctgccgctgg
      661 tgttcgtctg ccagaacaat cttttcgccg agatgacgcc gacgtcggaa accatgaagc
      721 tcgagcacgt cgccgaacgg gcttccggat acggcatgcc gggtgtccgc gtcgacggca
      781 atgatccgtt ggcggtgaaa gcggtgctcg acgaggcact gcggcgagcc cgccgcgccg
      841 gggggcccac gttcatcgag tgcgtcacct tccgcttccg tggccattac ttcggtgatc
      901 ggatgccgta tatcccaaaa gatcagctgg ccgcggcgat ggcggccgat ccggtgccgc
      961 ggttccgcga ccacctcgcc gaagccggcg tctgcagtct cgacgaactc gaccgcatcg
     1021 acaccgacgc cctggcaacg gtggaagcgg cattgaacac cgtcatgagt gccgagtgcc
     1081 cgtcgatcga cgaactcgac cgcgacgtat acgccaaccc catcaagttc ccggtgtgag
     1141 gcccgcagga aagaaagatg atggacgaaa aagagatgac gatgcgcgag gcgctcaacc
     1201 tcgcgctcga ccaagccctg ggggctgatg accgggtgtt cttgctcggt gaggacattg
     1261 ccgacccggg cgcagccgga ccgaccgcgg ggctgtcgac caagtacggc cgcgaacgcg
     1321 tgttggacac gcccatctcg gaagccgcaa tccttggcgc cgccgtcggt gcggcgatag
     1381 acggcctgct gccggtcgcc gagatcatga tcatggattt catcggcatc gccgccgacc
     1441 agctgatcaa ccacgcggcc aaactgcggt tcatgaccgc ggggcgaacg acggcgccga
     1501 tcaccgttcg gacccaggtt tacgccgggc tggccaccgg cgccacacat tcgcagagcc
     1561 tggaagcgtg gttcatgcac attcccggca tgaaggtgat cgtgccctcg acaccgcgcg
     1621 acggcaaagg cctgctgaca gccgcgatct tcgacgagga cccctgcctg ttcatcgaga
     1681 cgatccggct gcaaagtaag aaaggacccg tcccggtcca gcccgggttc tcaattccgc
     1741 tcggccaggc cgacatcaaa cggcacggca gcgatgtcac gctgatcagc tacgggcgca
     1801 gcgtgcatga cgcgctcgcg gcggccgccg cattggccga gcaagccgtc agcgcggagg
     1861 tcgtcgattt gcgcacgctg gtgccgctcg acgtcgagac catcatcgaa tccgcccggc
     1921 gcacaagacg agtcgtcatc gcccacgacg cggtgcaatt ctgcggtcct ggggccgaga
     1981 tagcggcgat cctgcagtcc gaattgttcg gcgagctggc cgcccccgtc gagcgggtgg
     2041 gggccaggtt cgtccccaac ccggccgcgg cggcgctcga ggcgcaggtg tgtccgtcgc
     2101 cggcccgcat catcgcggcc gcccagcgca ccctggacaa ggctaacgtc cgtggctgac
     2161 ttcatcattc ggattccgcg catctcggtg gctgtttcgg aagccgagct caccggactt
     2221 ctcgtcgagg caggtcagca tgtcgacgag ggcacgccga tctacgtgat cgccaccgag
     2281 aaggcggaac aggaaatcga agccggagcc tcaggcaccg tccaatggac gggccacgtc
     2341 gggaccacct acgacatcgg cgccgaaatc ggcgtcatca cctcatcacg gtaaacagcg
     2401 gaaggcaaaa cccatggatc tcaacgagac ccgcgagaga atcatctacg aaaaagaagg
     2461 cccgatcgcg cgcgtcaccc tgaactggcc cgagaaggcc aacgcccagg accagaaact
     2521 ggccgaagaa gtcgacgccg cactgctgga cgccgaccgc gactacgaca tcaaagtgct
     2581 gatcctcaag gccaacggca agggcttctg ctcgggacac gccatcggca acaatgccgt
     2641 cgactatccg gctttcgtcg aggccgcaaa ggtcatgggc acgccgtgga agccgcagac
     2701 cgacctgttc gtcaagccga tcctgaatct gtgggagttc tcaaagccga ccatcgcggc
     2761 cgtgcacggc tactgcgtcg gcggcggcac ccactacggg ctgaccaccg acatcgtcat
     2821 cgcctccgag gacgcgtact tctcctaccc gcccctgcag ggattcggca tgccgtccgg
     2881 tgaatgctca atcgagccat gggttttcat gaactggcgg cgcgcggcgt attaccttta
     2941 cctcgcagag gtgattgacg ccaagagagc tctggaggtc gggctggtca acgaggtcgt
     3001 tccgcgggag caactcaacg accgggtcga ggcgatcgcc cggcatatcg cccaggcgcc
     3061 gatgactacg ctgctggcga ccaaggccaa cctcaagcgg gcctgggagc tgatggggat
     3121 gcgggtgcac tggcagagct ccaacgacct cgtcgcgctg gcgtcgatca gcaaggatgt
     3181 gcaacagctg attcagcagg tgttcaagga caaggtgctg ccgtcggagc aggcccggcg
     3241 ccaggcggcc gccgccgcct cgaccgacgg cgcttcggcc acttgatttt tcgagacccg
     3301 gttagaggtc cctcggtgaa tatcgccgat catgcgacca ccgccggcca ggcgccggcg
     3361 ctgatcatgc ccggcggcgc caccatcacg ttcggcgagc tttatgcgag gagccagcgg
     3421 gtcgccgcgg tgctgcacag tgccggactg cgccgcggtg acggcgtggc cctggttctg
     3481 ccgaaccggc cagagttcgt cgaaatcacc tggggctgcc agctttccgg cctctactac
     3541 accgccgtca acacccattt cactcccggc gaggtcgcct acgtcatcga tgactctgac
     3601 gcaaaggcgg tgtttttgga cgcttcggac gggtcgatgg ccgaactcgc ggcgcacatt
     3661 tgcgacatca acgccgcggt cgaagtccat ataggcgtcg gaggcaacct gcccgactgg
     3721 ctagcgtatg agaacgcgct ggccccggcg ggcgatgcgc cgccggtgtc ggacgggtcg
     3781 gagatgctct actcgtcggg cactacagga cggcccaagg ccgtgcgtcg cccgctcccg
     3841 accgacggca acggctcatg ggcccaggcg gtcctggaga tggcgctggt gcacaagtac
     3901 gggatgaacc gatcgagcgt gtacctctcc cccgcgccgc tctatcacgc cgctggagtg
     3961 aactacacga tggcggtcaa tcgtgtgggg gcggcatcga tcatcatggg caagttcgac
     4021 gccgagacag tcttgcggct gatcgagacc cacagcgtca cccatgccca attcgtgccg
     4081 acaatgttcg tgcggatgct caagctgccg gactcggtcc ggctgaagta cgacgtttcg
     4141 agcctgcgat gcgtgatcca cgcggccgcc ccctgcccgg tcgatgtcaa gcatcggatg
     4201 atgaagtggt tcggaccgat catccacgaa tattacggtg gcactgaggg attcgcggga
     4261 acaacgatcg gccctacgga ctggctcgct catcccggtt cggtaggcat accgatggca
     4321 ccggtgcacg tggtcggcgc ggacgggcag gagcttcccg tcgggcagtc cggagagctt
     4381 tacttcgaag gtgggcctga cttcgagtac ttcaaggacc ccgccaagac cgcgtcggtg
     4441 tacaacgacc gcgggtggcg ctcgttgggc gacgtgggct acgtggacga ggacggctac
     4501 atgtacctga ccgaccggtc gacgttcatg atcgtctccg gtggagtgaa catctacccg
     4561 caggaggtgg agaacctgct ggtcatgcac cccaagctgg tcgatgccgc ggtgttcggc
     4621 gttcccaacc acgagttcgg cgaggaggtc aaagccgtcg tacaacctgc cgccggcgtc
     4681 gtatccgggc ccgacctcga agccgagctc atccaatact gccgagcgca gctcgccggc
     4741 tataagtgcc cgcgcacagt cgaattcgac ccgaaattac cgcgggaccc gaacggaaag
     4801 ctctacaaga ggcggatccg agaccggtac tggcaggggc gagcatcacg gattgtgtga
     4861 gccgatacaa gggatggcga catggcagtt gagacgggtg caccccgaac cgaagtgaat
     4921 tggacaccgc tacccgtgcc gtgggcggtc cagtccccca atcggatccc caagcagcgg
     4981 tactacgacg cggagttcta cgcgctggag gccgagatgt tctggccacg cgtgtggcag
     5041 ttggcatgtc gcctcgagga gatacccaag ccgggagact tcgtggagta cgagatcctc
     5101 gacgagtcga tcatcgtggt acgagtcgac actcagaccg tgcgggccta ccacaacgca
     5161 tgccgtcatc gaggcgtaaa gctggtggag ggcaacgggt ctcgacgcgc cttcgtctgc
     5221 ccattccacg gctggtgctg gagcatcgac ggacgcaaca cgtttgtggc tcgcccggag
     5281 gtgttcgccg aggagaacct gtgcccggac gatattcaac tcgtctcggt caagtgcgag
     5341 ctgtggggag gatgcgcgtg gatcaacctc gacgacggtg ccccagcgtt gcgcgactgc
     5401 atcgagccgt tcgcatcgat ctacgacgcc tggaaggttg aatcgctgcg ggtcgagtgg
     5461 tggcaatcgt gccggctgcc ggtgaattgg aagttggcga ccgcggcgtt catggagggc
     5521 taccacgtcc cgcaaaccca ccctcagctg ctgccgccgg cgcagatcgg cgggcctgct
     5581 gccgcagtgc atcccgtcat tcagaacagc ctgtatttca tgcgcactct cggagacggc
     5641 atgggcggca tgacccacga aagcgacatc cgcatcgccg agggccttca gcacctcgaa
     5701 ctaccgaccg attcaacgga agcgatgacc gcctggcgca gcgccctcaa cgacgccgtc
     5761 gtgggctggc atcgagcccg cggcagcacc atccccgatc tcaacgacct gcttcgtcgc
     5821 ggaatcaccg gcgcgatcgg attctgcttc ccgcaccact tcatcctgcc cacctacagc
     5881 agcgcctcgt cgtaccggat ccgtccgctg gggcccgagg agaccctgtt cgaaatctgg
     5941 tccctcacgc gtttcccctc cgaccactcc gcgggaaagc ccacaccgcc ggagcccatg
     6001 gcgcccgacg acccgcgctg gccgccgatc cccgcccaag acttctccaa cctgccgcga
     6061 cagcagaaag gcttgcattc caaagggttt gaatacatgc gattgtccaa tcagatcgaa
     6121 ggactcattt cgaacttcga acgcgtcgtc gacggtttcc tcgccggcct gtcctacgag
     6181 acgctggtgc ccgcggttca gaaaaccaac accaccatcg atgtgccgat cgccgacctg
     6241 ggattcacgc aggagccgcg atgaccgctc gctgggaccg ttccgtcgac ctgctgatcg
     6301 cgggcagcgg cggcggcggg atggtggcag ggttggcggc actcgactgc gggctggagc
     6361 cactgatcat cgaaaaacag ccgctggtcg gcggttcgac ggggctttcg ggcggcattg
     6421 tctggatgcc gaacaacccg ttgatgcggg ccgatggcat cgcggactcg cacgaggatg
     6481 gactggccta cctggccgac gtggtcggcg atatcggtgc gccctcctcg cccgcccgcc
     6541 gcgaaatgtt cctcaccgcg ggctacgaca tgatcaactt ccttctgcgc aaggacgttc
     6601 ggctgatccg ctgtgccgga tggagcgact actaccctaa tcacaagggc ggcaatgcgt
     6661 ctggccgcgc ggtcgaagga accccgttcg acgccgccga gttgggtagt tggcgcgaca
     6721 aggtgcaacc gccgctggcc aaaaactacg ggtatgtggt gctgaccaac gaactgcgct
     6781 ccgtccagta tttcaaccgt tcgccgcacg cattcacggt cgcaaccaag gtgttcctgc
     6841 gcaccgtggc ggcgcggatg cgccgccacc agatcctcac caatggggcg tcattgatag
     6901 gccagatgct gaaaatgctt atcgacctca ctgacggcca gcccccgttg tggacaaaca
     6961 ccgcgatgga cgatctcatc gtcgaggacg gcagggtcgt cggcgcccgc gtcacccgcg
     7021 acggcgcaac gctggacatc gaggcgcgca ggggcgtgct gctggctgcc ggcggcttcg
     7081 gccacaacgc agacatgcgc cgccgataca gcggcaatca acccaatgag gcacaatggt
     7141 cgatcgccaa cgccggggac accggcgagg tgcttcaggc cgcgatgagg gtgggcgccc
     7201 aaaccgacct gctagacgaa gcctggtggc tgccttcggt tttcatcgcc aacggcggcg
     7261 ccgccgccgc ctcgctcggg tccggtcggc agcggccggg agccatctac gtcgactcga
     7321 ccggccggcg gttctgcaac gagtcgaact cctacgtcga ggtcggcaag gcgatgtacg
     7381 ccaataaggc ggtgccctgc tggatgatct tcgacgacgg ttacgtacgc agatatgtcg
     7441 ccagcgccaa tccatttaaa cggaatcagc acctacctcc cgaactgatc gacaacggcg
     7501 cggtcaaacg ggccgacacc accgccgagc tggcgcgcca gatcgacctt cccgccgaag
     7561 aactggcgcg cacgatccag cgattcaacc agttcgccgc caagggcctc gaccccgatt
     7621 tcgggcgcgg ccaatcggcc tacaacgact gtctgggcga tcccgggtat cggccaaacg
     7681 ccgccgtcgg ccccctcgac cgcgcgccgt attacgcgac cagggtcttc ccggcggacg
     7741 tcgggacgtg cggcggcgtg atcaccaacg agcacgcaca ggtcctggac gagcaggacc
     7801 tggtgatcga aggcctgtac gccaccggga acaccaccgc gacggtgatg ggccgaacgt
     7861 atccgggcgc cggcgcgagc atcgccagct cgatggtgtt cggctacgtc gccgcccggc
     7921 atgccgccgg ccgcaagatc gcgggcgaga tgagccgcta gtcccccgca agtcgggccg
     7981 agcgtgacgc tgtcgtcacg ctcggcctcg aatgtgactg cagcgtcacg ttggcggggc
     8041 gtccggttcg tcgtggccga cgaat
//
LOCUS       DASSGQ010000074         4945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_399997,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000074 DASSGQ010000000
VERSION     DASSGQ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399997"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2234
                     /locus_tag="VFI55_03325"
     CDS             <1..2234
                     /locus_tag="VFI55_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET7073097.1"
                     /translation="AVVRLAAISPGAVADMVQDVVRARVSESLLNLAAKAHGNPFLVS
                     ELVGGLGEEGRLNVSGGRAVATGEALPRRLGASMQHRLDLLSEGAGEVVRVAAVLPDR
                     FSAGLLAAMLERQPSSLISAVGEVVRADLLVEDGEQLRFRHDLLREATRQSLPQSLRR
                     AMERQSASIMLGMGAAPPEVATQLARSADPGDREAISALRQAAQSVGHSDASAAADLS
                     KRALELLPGEDPDQGLLVAETVELLNRASRYEEAEELAVVALSEVASPEQEAEIRLRL
                     PTLNKHTNQRRIEENRRALRLLNISEVTRARHLAWLAYNLTFQNQTAQQRVAADEAAA
                     AAASTGDLESKMVVSLTLALLDAGEGYAGGALRRLEELCALARTSDATAAHDLAAMHH
                     ALLLAAVGRLDDAAARAAEGTEQARREGNSMALAMWAAVDAVVHLSAGRLSAARAAAE
                     SLPPPRRAGVSEPDMIRMVILAEVAALTDDRTLLQQMVNAAHEAYSNRSSAVRRGAAH
                     VLALAAWQRDDVDDAMRWLGGDIALFRTPLWPQVLDRVILTARIASAGGDAGLRARVL
                     HAIDSLEREQPGIPLFTGVAGCARGVLERDAQALQAAADLLRSSSRPLFYAAAAEDAG
                     TELARIDRRDEAVERLNAAFDTYLHYGALADARRVGRELRRLGVERRIVSQARAKTGW
                     DSLTDAELKVVNLIAQGATNRDVAAQLHLSLSTVKTHVHNAFAKLGINSRTQLTQVTR
                     GSD"
     gene            2533..2676
                     /locus_tag="VFI55_03330"
     CDS             2533..2676
                     /locus_tag="VFI55_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014712137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long chain fatty acid-CoA synthetase Faa4p"
                     /protein_id="HET7073098.1"
                     /translation="MIRVPEIGGIARASRRAQVEVVARECIAARTGIPIGYVAIFVAR
                     EIG"
     gene            complement(2770..>4945)
                     /locus_tag="VFI55_03335"
     CDS             complement(2770..>4945)
                     /locus_tag="VFI55_03335"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005110390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HET7073099.1"
                     /translation="REALTAMGQNLDTVLACEEEPGLGNGGLGRLAACYLDSLATLER
                     PAIGYGIRYEFGIFDQEIHDGWQVEKTDNWLDSGNPWEIPKPDVNYLVKWGGYAEHYT
                     DDAGHERIRWVPGRMLKGVAYDTPIQGYGVNTCNVLTLWSARAVKSFALDAFNTGDYY
                     KAVEDEVTSETVTKVLYPNDEPEAGKRLRLLQQYFFVSCSLQHVLHIMDDLADAGVRE
                     LPQRFALQLNDTHPSIGVAELMRLLVDERQLEWEEAWEITVATFGYTNHTLLPEALET
                     WPLEMFAESLPRHLEIIYEINRRFLDEVRAKFPGDEDRIRRMSLIGEDGGKKVRMAHL
                     ATVGSHAVNGVAALHSELLKASVLKDFYELWPERFSNKTNGVTPRRFLALANPGLREL
                     LDRTVGEGWLTDLKRLRGLESFVDDHSFRAQWRDIKRTNKARLATYIRSVAGVELNPD
                     WMFDVQVKRIHEYKRQHLNVLHIVALYHRLKCNPGLSIPQRAFIFGGKAAPGYFMAKR
                     IIKLINAVGETVNADPDVNRFIKVAFVPNFNVQNAHLIYPAADLSEQISTAGKEASGT
                     GNMKFMINGALTIGTLDGANVEIREEVGAENFFLFGLTESEVEAVKARGYRPADYIAA
                     NDELREVLDLIAGGTFSHGDTEVFRPLVDNLRYDDPFLVCADFASYVSCQDQVSTAWR
                     DKESWTKMSILNTARSGKFSSDRAITEYCDDIWNVWPLTVTM"
BASE COUNT          764 a         1577 c         1717 g          887 t
ORIGIN      
        1 ccgccgttgt gcgactggcc gcgatatcgc cgggcgcggt cgccgacatg gtgcaggacg
       61 tcgtgcgggc cagggtgagt gagtcgctgc tgaatctggc cgccaaggcg cacgggaacc
      121 cgtttttggt cagcgagctg gtggggggtc tgggcgagga gggccggctc aatgtgagcg
      181 gcggccgggc ggtggccacc ggggaggcgc tgccgcgccg gctgggcgcc agcatgcagc
      241 accgattgga cctgctctcc gagggtgccg gcgaggtggt gcgggtggcc gcggtgttgc
      301 cggaccggtt ttcggcgggg ctgctggccg cgatgctgga acgccagccg tcgtcgttga
      361 tctcggctgt cggggaggtc gtccgcgcgg acctgctcgt cgaagatggt gagcagttgc
      421 ggtttcggca tgacctgctg cgggaggcca cccggcagtc gttgccgcag tcgctgcggc
      481 gtgcgatgga gcgccagtcg gcgtcgatca tgctggggat gggcgcggcg ccgcccgagg
      541 tggccactca gctggcgcgc agcgccgacc cgggagaccg ggaggcgatc agtgcgctgc
      601 ggcaggcggc gcaatcggtt gggcacagcg atgccagtgc ggccgcggat ttgagcaaac
      661 gcgcattgga gctgttgccc ggcgaagatc ctgaccaagg actcctggtc gccgaaacgg
      721 tggagttgct caaccgggcc agccgctacg aggaggccga agagttggcc gttgtggcgc
      781 tctcggaggt ggcgtcgccc gaacaagagg ccgagattcg cttgcgcctc ccgacgctca
      841 acaagcacac caaccagcgg cgtatcgagg agaatcgtcg tgcgctgcgg ttactcaaca
      901 tcagcgaggt cacccgggca cggcacctgg cgtggctggc ctacaacctg acctttcaga
      961 accaaaccgc acagcagcgc gtggcggccg atgaggctgc cgccgctgcg gcgtccacgg
     1021 gtgatctcga gtcgaaaatg gtggtcagcc ttacccttgc cttgctcgac gccggcgagg
     1081 ggtacgcggg cggcgccctg cgccgcctcg aggagctctg cgcgctcgcc cgcacgagtg
     1141 acgcgaccgc agctcatgac ttggccgcca tgcaccacgc gctcctgctg gcggcggttg
     1201 gccggttgga cgatgccgcg gcccgcgccg ctgaggggac agagcaagcc cgccgggaag
     1261 gcaactcgat ggccctggca atgtgggccg cggttgacgc ggtggtccac ttatctgcgg
     1321 gccggttgtc tgctgcgcgc gccgcggccg agtctctgcc gcccccgcgg cgggcaggcg
     1381 tatccgagcc ggacatgatc cgcatggtga ttctggccga ggtggcggct ctcacggatg
     1441 accgaacgtt gttgcagcag atggtcaatg ccgcgcacga agcctactcc aatcgctcat
     1501 ctgcggtacg tcgtggagcg gcgcacgtgc tcgcgctcgc ggcgtggcag cgtgacgacg
     1561 tcgacgacgc gatgcgctgg ctgggcggcg acatcgccct gttccggaca ccactctggc
     1621 cgcaggtctt agatcgggtg atcttgactg cgcggattgc gtcggccggc ggcgatgccg
     1681 gcttgcgcgc gcgtgtgctg cacgcgattg actcgctcga acgtgagcag ccggggatcc
     1741 cgctgttcac cggggtcgcg gggtgtgccc gcggggtcct cgaacgcgat gcccaggcac
     1801 tgcaggccgc cgcggatttg cttcggtcgt cgtcgcggcc gctcttctat gccgcggccg
     1861 ccgaggacgc cggaactgaa ctggcgcgca tcgaccgtcg tgatgaggcg gttgaacgac
     1921 tcaatgcggc ctttgacacc tacctgcact acggagcgct cgccgatgcg cgtcgggtgg
     1981 gtcgcgagtt gcgccggttg ggtgtggagc ggcgcatcgt cagccaggcg cgggcgaaga
     2041 cgggctggga cagcctcacc gacgccgagc tcaaggtcgt caacctcatc gcgcagggtg
     2101 ccaccaaccg cgacgtcgcg gcccaactgc acctttccct gtccacggtg aaaactcatg
     2161 tccacaatgc gttcgccaag ctgggcatca actcccgcac ccaattgacg caggtcacgc
     2221 ggggatctga ttgatcggcc cggcactagc gcggactcca agatcggccg caccgttagc
     2281 actggtcgac accattgttt cggccgcggt cacgatgacc acctcagcgg attaggttct
     2341 tggccaaaga cggattaccc ttggccgcgg cctgtcatcg actcaggaaa aatccgtatg
     2401 cctgttcagc ccactccgcg acgcacacaa ccactgcgcg ggagtcgtgc ccaacgtgtc
     2461 gcccatcgta agaccttccc gacggtcggt cagtgctttg aagtggagat cacccgcgag
     2521 gcggacgggt ggatgatccg ggtccccgaa attggtggga tcgcacgcgc gagtcgtcgc
     2581 gctcaggtcg aggtggtggc gcgggaatgc atcgccgccc ggacgggcat cccgatcggg
     2641 tacgtcgcca tctttgtggc gcgcgagatc ggttagtgcc gagcggcttt cgctgcgcga
     2701 ctgggtgaat gcccggcgag cgcgtttggc cggcgggtac tagtgcgcgg ctgatcgggc
     2761 agcccagatt cacatggtta cggtcagcgg ccagacattc cagatgtcgt cgcagtactc
     2821 ggtgatggcg cgatccgagg agaacttgcc gctgcgcgcg gtgttgagga tggacatctt
     2881 cgtccacgac tccttgtccc gccacgcggt gctcacctgg tcctggcagc tgacgtagga
     2941 ggcgaagtcg gcgcacacca ggaaggggtc gtcgtagcgg aggttgtcga ccagcggccg
     3001 gaacacctcg gtgtcgccgt gcgagaacgt accgccggca atcagatcca gcacctcgcg
     3061 cagctcgtcg ttggccgcga tgtagtcggc cgggcggtag ccgcgtgcct tgacggcctc
     3121 gacctcggac tcggtcagac cgaacaggaa gaaattctcc gcgccgacct cttcgcgtat
     3181 ctcgacgttg gccccgtcca gtgtgccgat ggtcagcgct ccgttgatca tgaacttcat
     3241 gttcccggtg cccgaggctt ccttgcccgc ggtggagatc tgttcggaca gatcggcggc
     3301 cgggtagatc aggtgcgcgt tctgcacgtt gaagttcggc acgaaggcca ccttgatgaa
     3361 ccggttcacg tccgggtcgg cgttgaccgt ctcaccgacg gcgttgatca gcttgatgat
     3421 ccgcttggcc atgaagtagc ccggagcggc cttaccgccg aagatgaacg cccgctgcgg
     3481 aatcgacagt ccggggttgc acttgagccg gtggtagagc gcgacgatgt gcaggacgtt
     3541 gaggtgctga cgcttgtact cgtggattcg cttgacctga acgtcgaaca tccagtccgg
     3601 gttcagttcg acgccggcca ccgatcggat gtacgtggcg agccgtgcct tgttggtgcg
     3661 tttgatgtcc cgccattgcg cacggaacga gtggtcgtcg acgaacgact ccagcccgcg
     3721 cagccgcttc agatcggtca gccagccttc accgacggtg cggtccagca gctcgcgcag
     3781 gccggggttg gccagcgcga ggaaacggcg cggcgtcacg ccgttcgtct tgttgctgaa
     3841 ccgctccggc cacagctcgt aaaagtcttt cagcacactg gctttcagca gctccgagtg
     3901 cagcgcggcg acgccgttga cggcgtggct gcccaccgtc gccaggtgcg ccatccggac
     3961 cttcttgccg ccgtcctcgc cgatcagcga catccggcga atccgatctt cgtcgccggg
     4021 gaacttggcg cgcacctcgt cgaggaagcg gcggttgatc tcgtagatga tctcgaggtg
     4081 gcggggcagc gactccgcga acatctccag cggccaggtc tcgagcgcct cgggcagcag
     4141 ggtgtggttg gtgtagccga acgtcgcaac cgtgatctcc cacgcctctt cccactccag
     4201 ctggcgctcg tcgaccagca accgcatcag ctcggccacc ccgatcgagg ggtgagtgtc
     4261 gttgagctgc agggcgaacc gctgggggag ttcccggacg ccggcgtccg cgaggtcgtc
     4321 catgatgtgc agcacgtgct gcagcgagca ggacacgaag aagtactgct gcagcaggcg
     4381 cagccgcttg cccgcctccg gctcgtcgtt ggggtacagc accttggtga ccgtctcaga
     4441 ggtgacctcg tcctcgaccg ccttgtagta gtcgccggtg ttgaaggcgt ctaacgcgaa
     4501 cgacttgacg gcccgcgcac tccacagcgt cagcacgttg catgtgttga cgccgtaacc
     4561 ctggatgggc gtgtcgtagg cgacgccttt cagcatccgc cccggcaccc accggatgcg
     4621 ctcgtggccg gcgtcatcgg tgtagtgctc ggcgtagccg ccccacttga cgaggtagtt
     4681 gacgtcgggt ttgggtatct cccaagggtt tccgctgtcc agccagttgt cggtcttctc
     4741 cacctgccag ccgtcgtgga tctcctggtc gaagatgccg aattcgtagc ggatcccgta
     4801 gccgatcgcc ggacgctcga gggtggccag cgagtctagg tagcacgcgg cgagcctgcc
     4861 taagccgccg ttgcccaatc ccggctcctc ttcgcaggcc aggaccgtgt cgaggttctg
     4921 gcccatcgcc gtgagcgcct cccgg
//
LOCUS       DASSGQ010000075        29196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_947419,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000075 DASSGQ010000000
VERSION     DASSGQ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 29196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29196
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947419"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VFI55_03340"
     CDS             <1..450
                     /locus_tag="VFI55_03340"
                     /inference="COORDINATES: protein motif:HMM:NF023900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE/PPE C-terminal domain-containing protein"
                     /protein_id="HET7073100.1"
                     /translation="NPAIIEPAVTAAMADVNAVSVAAQPAATVLPPMGSGSGNGTWLP
                     IGSAGLGVGGVPGADLSGVSAGISRAAFVGRLSVPQSWTEAVSVVNHSGAALPGGGWI
                     GTAVPDSSVGMPGMPGMPMARTAGYSFGHGPRYGFQPTVMPRPPAAG"
     gene            737..1864
                     /gene="pstS"
                     /locus_tag="VFI55_03345"
     CDS             737..1864
                     /gene="pstS"
                     /locus_tag="VFI55_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET7073101.1"
                     /translation="MKHSRSAAAMSLLTAAALALSSCSNSHTTLAYAAGAKVDCGGKQ
                     TLAASGSTAQANAMTKFIDAYHIACSGQTLSYTANGSGAGISDFLAGKTDFGGSDTPL
                     SGDQYTAAKQRCGGADAWNLPVVFGPMAITYNLSAVDSLVLDAPTLAKIFNGTITRWD
                     DPALTALNASMPAEDIRVIYRSDASGTTYNFQSYLQAASGGAWNQGAAKTFNGGVGTG
                     AAGNSGTAALVKTTEGAISYNELSFALQQGLYAAEIKTPASRRSLRPVRIGTDTVGKT
                     ITGAKITGSGNDLVLDISSFYNPAQPDVYPIVMATYEIVCSKYPNADVAKAVKAFLQA
                     AIGPGQVDLNRIGYIPLSPDFQARVSNAVDAISAAGTTNSE"
     gene            complement(1930..4281)
                     /locus_tag="VFI55_03350"
     CDS             complement(1930..4281)
                     /locus_tag="VFI55_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET7073102.1"
                     /translation="MATEFTGKIALDIRDSEPDWGPYAAPTAPANSPNILYLVWDDTG
                     IATWDCFGGLVEMPAMTRIAARGVRLSQFHTTALCSPTRASLLTGRNATTVGMATIEE
                     FTDGFPNCNGRIPADTALLSEVLAERGYNTYCVGKWHLTPLEESNLASTKRHWPTSRG
                     FERFYGFMGGETDQWYPDLVYDNHPVNPPGTPEDGYHLSKDIADKTIEFIRDAKVIAP
                     DKPWFSYVCPGAGHAPHHVFKDWADKYAGRFDMGYERYREIVLENQKSLGIVPPDTEL
                     SPVNPYLDVKGPQGEPWPLQDTVRPWDSLNDEEKNLFSRMAEVFAGFLSYTDAQIGRI
                     LDYLEESGQLDNTIIVVISDNGASGEGGPNGSVNEGKFFNGYIDTVEESMKLFDHLGG
                     PETYNHYPIGWAMAFNTPYKLYKRYASHEGGIADTAIISWPNGIAAHGEVRDNYVNVC
                     DITPTVYDLLDMTAPETVKGITQTPLDGISFKAALADSSTDTGKETQFYTMLGTRGIW
                     HKGWFANTVHAATPAGWSHFDADRWELFHIEADRSQCHDLAAEMPDKLEELKALWFSE
                     AAKYNGLPLADLNLLETLTRFRPYLIGERDSYIYYPDCADVGIGAAVEIRGRSFAVLA
                     DVTVDTTGAEGVLFKQGGGHGGHVLFIQDGRLHYVYNFLGERQQLISSSGPVPLGRHV
                     FGVRYERTGTVENSHTPVGEVTLFIDDTLVGSLAGVTAHPGTFGLAGAGITVGRNGGS
                     GVSSRYKAPFAFTGGTIAQVTVDVSGRPYEDVERELALAFSRD"
     gene            complement(4546..4908)
                     /gene="rpsQ"
                     /locus_tag="VFI55_03355"
     CDS             complement(4546..4908)
                     /gene="rpsQ"
                     /locus_tag="VFI55_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET7073103.1"
                     /translation="MAEAKVASSAAKSAPKAAPEAAGSKDKGPKHTPPTPTARGRRKV
                     RIGYVVSDKMQKTIVVELEDRMRHPLYGKIIRTTKKVKAHDENSVAGIGDRVSLMETR
                     PMSATKRWRLVEILERAK"
     gene            complement(4908..5153)
                     /gene="rpmC"
                     /locus_tag="VFI55_03360"
     CDS             complement(4908..5153)
                     /gene="rpmC"
                     /locus_tag="VFI55_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013830257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET7073104.1"
                     /translation="MAVGISPGELRELTDEELAERVRESKEELFNLRFQMATGQLTNN
                     RRLRVVRHEIARVYTVLRERELGLASGPNGSDGNKEA"
     gene            complement(5153..5569)
                     /gene="rplP"
                     /locus_tag="VFI55_03365"
     CDS             complement(5153..5569)
                     /gene="rplP"
                     /locus_tag="VFI55_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET7073105.1"
                     /translation="MLIPRKVKHRKQHHPRQRGIASGGTSVNFGDYGIQALEHAYVTN
                     RQIESARIAINRHIKRGGKVWINVFPDRPLTKKPAETRMGSGKGSPEWWVVNVKPGRV
                     LFELSYPNEQIARAALTRAIHKLPIKARIVTREEQF"
     gene            complement(5573..6430)
                     /gene="rpsC"
                     /locus_tag="VFI55_03370"
     CDS             complement(5573..6430)
                     /gene="rpsC"
                     /locus_tag="VFI55_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019737224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET7073106.1"
                     /translation="MGQKINPHGFRLGITTDWKSRWYADKQYAEYVKEDVAIRRLLSS
                     GLERAGIADVEIERTRDRVRVDIHTARPGIVIGRRGTEADRIRADLEKLTKKQVQLNI
                     LEVKNPESQAQLVAQGVAEQLSNRVAFRRAMRKAIQSAMRQPNVKGIRVQCSGRLGGA
                     EMSRSEFYREGRVPLHTLRADIDYGLYEAKTTFGRIGVKVWIYKGDIVGGKRELAVAA
                     PAGAERPRRERPSGTRPRRSGASGTTATSTEAGRAAGAEESTTSGDAAPTVSSLEPQS
                     TESTESTES"
     gene            complement(6430..6987)
                     /gene="rplV"
                     /locus_tag="VFI55_03375"
     CDS             complement(6430..6987)
                     /gene="rplV"
                     /locus_tag="VFI55_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET7073107.1"
                     /translation="MSTTTTEFPSATAVARFVRVSPTKARRVINLVRGRSVTDALDIL
                     RWAPQAASEPVAKVIASAAANAQNNNGLDPATLVVATVYADEGPTAKRIRPRAQGRAF
                     RIRRRTSHITVVVESRPAKDQGSAKSTRARRAEASKAAAKKAPANKTAAKKAPAKSAE
                     AKATGKTTGTATATAQTSEAKGGSD"
     gene            complement(6984..7265)
                     /gene="rpsS"
                     /locus_tag="VFI55_03380"
     CDS             complement(6984..7265)
                     /gene="rpsS"
                     /locus_tag="VFI55_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET7073108.1"
                     /translation="MPRSLKKGPFVDDHLLKKVDVQNEKNSKQVIKTWSRRSTIIPDF
                     IGHTFAVHDGRKHVPVFVTEAMVGHKLGEFAPTRTFKGHIKDDRKAKRR"
     gene            complement(7329..8171)
                     /gene="rplB"
                     /locus_tag="VFI55_03385"
     CDS             complement(7329..8171)
                     /gene="rplB"
                     /locus_tag="VFI55_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET7073109.1"
                     /translation="MAIRKYKPTTPGRRGASVSDFAEITRAQPEKSLVRPLHGHGGRN
                     AHGRITTRHQGGGHKRAYRVIDFRRNDKDGVNAKVAHIEYDPNRTANIALLHFLDGEK
                     RYIIAPQGLSQGDVVESGANADIKPGNNLPLRNIPAGTLIHAVELRPGGGAKLARSAG
                     SSIQLLGKEGTYASLRMPSGEIRRVDVRCRATVGEVGNAEQANINWGKAGRMRWKGKR
                     PSVRGVVMNPVDHPHGGGEGKTSGGRHPVSPWGKPEGRTRHPNKLSNKLIVRRRRTGK
                     KHSR"
     gene            complement(8199..8501)
                     /gene="rplW"
                     /locus_tag="VFI55_03390"
     CDS             complement(8199..8501)
                     /gene="rplW"
                     /locus_tag="VFI55_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET7073110.1"
                     /translation="MATIADPRDIILAPVISEKSYGLLDDNVYTFVVHPDSNKTQIKI
                     AVEKIFSVKVASVNTANRQGKRKRTRTGFGKRKNSKRAIVTLAPGSKPIDLFGAPA"
     gene            complement(8501..9199)
                     /gene="rplD"
                     /locus_tag="VFI55_03395"
     CDS             complement(8501..9199)
                     /gene="rplD"
                     /locus_tag="VFI55_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET7073111.1"
                     /translation="MAAQEDQKGLKIQVKTPGGKVDGFIELPAELFDAPANIALMHQV
                     VTAQRAAARQGTHSTKTRGDVRGGGKKPYRQKGTGRARQGSTRAPQFTGGGVVHGPKP
                     RDYSQRTPKKMIAAALRGALSDRARNGRIHAVTELVAGQTPSTKSAKAFLDALTDRKQ
                     VLLIIGRTDETGTKSVRNLPGVHVLTPDQLNTYDVLRSDDVVFSVEALNAYIAANTAT
                     DTVADTASTEEVSA"
     gene            complement(9199..9852)
                     /gene="rplC"
                     /locus_tag="VFI55_03400"
     CDS             complement(9199..9852)
                     /gene="rplC"
                     /locus_tag="VFI55_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET7073112.1"
                     /translation="MARIGILGTKLGMTQVFDENNRIVPVTVVKAGPNVVTRIRTPER
                     DGYSAVQLAYGEISPRKVNKPVTGQYTAAGVNPRRYLAELRLDNPDAAAEYEVGQELT
                     AEIFADGNYVDVTGTSKGKGFAGTMKRHGFRGQGASHGAQAVHRRPGSIGGCATPARV
                     FKGTRMAGRMGNDRVTVQNLLVHKVDTENGVLLIKGAVPGRTGGLVMVRSAIKRGEK"
     gene            complement(9867..10172)
                     /gene="rpsJ"
                     /locus_tag="VFI55_03405"
     CDS             complement(9867..10172)
                     /gene="rpsJ"
                     /locus_tag="VFI55_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET7073113.1"
                     /translation="MAGQKIRIRLKAYDHEAIDASARKIVETVVRTGASVVGPVPLPT
                     EKNIYCVIRSPHKYKDSREHFEMRTHKRLIDILDPTPKTVDALMRIDLPASVDVNIQ"
     gene            10632..11894
                     /locus_tag="VFI55_03410"
     CDS             10632..11894
                     /locus_tag="VFI55_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014712393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7073114.1"
                     /translation="MGTPTVDPVSREAAKVFADPTAYADEPRLHAALTHLRAHAPVVP
                     VDCAPYRPFWAITKHADIMEIERANNLFINEPRPLLGTAAADDVARAQLDAGMGLRTL
                     IHMDDPQHRVVRAIGADWFRPKAMRALKVRTDELAKLYVDKMMAAGNECDFVQEVAVN
                     YPLYVIMSLLGLPESDFGRMLKLTQELFGGDDAEFRRGTSLDEQLQVLLDFFGYFNAL
                     TGSRRETPTEDLASAIANARVDGEPLSDVDTASYYVIIATAGHDTTSATIAGGLRALI
                     ENPDQRERLTSNLDLMPLATEEMIRWVTPVKEFMRTATKDTTIRGVPIAEGEAVYLAY
                     VSGNRDEEMFDDPFSFDVGRDPNKHLAFGYGVHFCLGAALARIEVNSFFSELLPRLKS
                     IELNGEPELIATTFVGGLKHLPIRYSLR"
     gene            12235..12492
                     /locus_tag="VFI55_03415"
     CDS             12235..12492
                     /locus_tag="VFI55_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073115.1"
                     /translation="MGIAAAGGLHRTGLQRGNRLAATAMAEVSKSAFLTTTHASMAVM
                     AVIVAVSALRIGRWAPGRDVQQLILVRRASTAAGSRAEPAY"
     gene            12599..13474
                     /locus_tag="VFI55_03420"
     CDS             12599..13474
                     /locus_tag="VFI55_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HET7073116.1"
                     /translation="MSYVIAAPEYVAAAATDLANIGSTISDANSVASGPVSSILPAGA
                     DEVSASIAALFDAHAQAYQALSAAAQAFHTQFVQLMSAGSEQYALTEAANSTPLQAAG
                     QGVLSGGHAPSQVLKAATTVQPVMGSAASAGSAAAGVPAAGASATGVAGGSVPAAPAA
                     PLTAAIAPISAPPAGLASTSSPAAAVPAGSAATPVSPLQAATPAYAPATADAVAATPV
                     GTPVAAVPAETPELSPAAATALPAATPVTAAPAATAAYSPATPPYSPATAASGSPREQ
                     SAAPAEYGEMTPYSA"
     gene            complement(13471..14916)
                     /gene="mftG"
                     /locus_tag="VFI55_03425"
     CDS             complement(13471..14916)
                     /gene="mftG"
                     /locus_tag="VFI55_03425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin system GMC family oxidoreductase
                     MftG"
                     /protein_id="HET7073117.1"
                     /translation="MTEAMLHSDVLIIGAGSAGSVVAERLSADPACVVTVLEAGPGLT
                     DPELLARTTNGLQLPIGVGSPLAQRYLTRLTDRHALQLPIVRGSTVGGSGAVNGGYFC
                     RGMPRDFDRAAIPGWAWSDVLDHFRAIETDLDFDGPAHGDSGPILVRRTRDMTGTTER
                     FIAAAGRAGFPWIADLNDCGPELTSGVGAVPLNIVDGVRIGSGAAYLIPALDRSNLTL
                     LAQTRAVRVRFAGSTAFGVDAIGPQGPTTFTATRIVLCAGAIQSAHLLMLSGIGDEAM
                     LRAAGVPVVSPLPVGMFCSDHPEWVLPTNWAVATGRPVIEVVLSTDDGIEIRPYTGGF
                     VAMTGDGTPGHPDWPHIGVALMQPRARGRVMLVSRDPGVPPRIEHRYDSEPDDVAALR
                     RGSELARELAGAETHVGSPVWSTSQHLCASVPMGADGDPRAVVDHRCRVRGIENLWVI
                     DGSILPSITSRGPHATIVMLGHRAAGFVRLN"
     gene            complement(14936..16348)
                     /gene="mftF"
                     /locus_tag="VFI55_03430"
     CDS             complement(14936..16348)
                     /gene="mftF"
                     /locus_tag="VFI55_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215210.1"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="HET7073118.1"
                     /translation="MSQPRLPDGFAVQVDRRVRVLGDGSALLGGSPTRLLKLAPAAQD
                     MLSDGRLKVRDDVSAQLARTLLDATVAHPRPSGGPSHRDVTVVIPVRNNVSGVRRLVS
                     SLRGLRVVVVDDGSSPPIEPADFVGAHCDVEVLRHTFSKGPAAARNTGLASCKTDFVA
                     FLDSDVAPRRGWLEALLGHFCDPTVALVAPRIVGLSQSENVVARYEAVHSSLDLGQRE
                     APVLPHSTVSYVPSAAIICRCSAIRDVGGFDETMQSGEDVDLCWRLIEAGARLRYEPI
                     ALVAHDHRTELRDWLARKAFYGGSAAPLSVRHPDKTAPVVISGWALMAWILMALGTSL
                     SQLTSIVIALLTGRRIARAMRSAETSMTDVLLIASRGLGSAALQLASALCRHYWPLAL
                     FAAILSRHFRRVVLVAAVMDGVVDWLRRRDASSDEIEPIGLLTYLLLKRVDDLAYGLG
                     LWWGVVRERNLRALKPQFRS"
     gene            complement(16345..17088)
                     /gene="mftE"
                     /locus_tag="VFI55_03435"
     CDS             complement(16345..17088)
                     /gene="mftE"
                     /locus_tag="VFI55_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="HET7073119.1"
                     /translation="MNSSYHQPVPVLGELGTATSSQLSSTSPSIMVPLGSTEQHGPHL
                     PLDTDTRIATAVARGAAAGLRTLLGHEWLVAPAIAYGASGEHQDFAGTISIGTDALTT
                     LLVEYGRSAACWAQRLVFVNGHGGNVGALRGAVRTLRAEGRDVGWSACAASGGDAHAG
                     HSETSLLLHISPAEVRSDRWLAGNRAPLAELIGPMRRGGVAAVSRIGVLGDPTTATAV
                     EGSRIFGEMVDGCVRRVARWLPGPDGMLT"
     gene            complement(17321..18511)
                     /gene="mftD"
                     /locus_tag="VFI55_03440"
     CDS             complement(17321..18511)
                     /gene="mftD"
                     /locus_tag="VFI55_03440"
                     /EC_number="1.4.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167825.1"
                     /note="MftD, an enzyme found in the mycofactocin
                     biosynthesis locus, performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the
                     non-exchangeable NADH of TIGR03971 family SDR-type
                     oxidoreductases; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pre-mycofactocin synthase MftD"
                     /protein_id="HET7073120.1"
                     /translation="MANEWFETVAVAQQRAKRRLPKSVYSSLISASEKGITVADNVAA
                     FSELGFAPHVIGASVKRDLSTTVMGQDLALPVIISPTGVQAVDPDGEVAVARAAAARG
                     TAMGLSSFASKPIEEVIAANPKVFFQVYWLGGRDAIAERVERARQAGAVGLIVTTDWT
                     FSHGRDWGSPEIPEAMNLRTVLRLSPEAILRPRWLWKFGKTLRPPQLRVPNQGRRGEA
                     GPPFFAAYGEWMGTPPPTWEDIAWLRELWGGPFMLKGVMRVDDAKRAVDAGVSAISVS
                     NHGGNNLDGTPASIRALPAVAAAVGDQVEVLLDGGIRRGGDVVKAVALGARAVMIGRA
                     YLWGLGAAGQAGVENVLDILRAGVDSALMGLGHSSVHDLSPDDVLVPPGFTRTLGAAS
                     AADA"
     gene            complement(18514..19689)
                     /gene="mftC"
                     /locus_tag="VFI55_03445"
     CDS             complement(18514..19689)
                     /gene="mftC"
                     /locus_tag="VFI55_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215207.1"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron
                     carrier mycofactocin from the terminal Val-Tyr dipeptide
                     of the precursor peptide MftA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="HET7073121.1"
                     /translation="MPRLIEQFERGLDAPICLTWELTYACNLACVHCLSSSGKRDPRE
                     LSTRQCQDVIDELERMQVFYVNIGGGEPTVRPDFWELVDYATAHHVGVKFSTNGVRIT
                     PEVAARLAASDYVDVQISLDGATAEVNDAVRGAGSFAMAVRALENLAAAGFADSGGGA
                     KISVVVTRHNVGQLDEFAALAHRYGATLRITRLRPSGRGADVWDDLHPTAAQQVALYD
                     WLVAKGERVLTGDSFFHLSPLGQSGALAGLNMCGAGRVVCLIDPVGDVYACPFAIHDR
                     FLAGNILSDGGFDNVWRNAPLFRELREPQSAGACGTCGHYDSCRGGCMAAKFFTGLPI
                     DGPDPECVQGHSASALSRERQTPRPRADHSHGQPGRQPVALTLSVRPPARPCNESPL"
     gene            complement(19707..19952)
                     /gene="mftB"
                     /locus_tag="VFI55_03450"
     CDS             complement(19707..19952)
                     /gene="mftB"
                     /locus_tag="VFI55_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814231.1"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ,
                     a cofactor that derives entirely from a Tyr and a Glu in
                     the precursor PqqA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="HET7073122.1"
                     /translation="MHPQVAIRPESFGALLYHFGTRKLSFLKNPTILTVVRSLADHAD
                     ARSACRAAGIGDAGQAPYLHALGVLAASNMLVAREDQ"
     gene            complement(20099..20188)
                     /gene="mftA"
                     /locus_tag="VFI55_03455"
     CDS             complement(20099..20188)
                     /gene="mftA"
                     /locus_tag="VFI55_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_007409348.2"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes
                     in Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin precursor MftA"
                     /protein_id="HET7073123.1"
                     /translation="MDHEIDTDSELVTETLVEEVSIDGMCGVY"
     gene            20283..20879
                     /gene="mftR"
                     /locus_tag="VFI55_03460"
     CDS             20283..20879
                     /gene="mftR"
                     /locus_tag="VFI55_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215205.1"
                     /note="MftR, the mycofactocin system transcriptional
                     regulator, is an uncharacterized TetR family DNA-binding
                     transcription factor. Its role is inferred by context. It
                     occurs as part of the biosynthesis locus for mycofactocin,
                     a partially characterized electron carrier derived from
                     the terminal Val-Tyr dipeptide of the precursor peptide
                     MftA, through a radical SAM enzyme-mediated process;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0036042 - long-chain fatty acyl-CoA
                     binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin system transcriptional regulator"
                     /protein_id="HET7073124.1"
                     /translation="MMPESRVGRRRSTTPHHITDVGIELFSARGFGEVSVDDVAQAAG
                     ISRRTLFRYYASKNAIPWGDFDTHLEHLRDLLDNIDRAVPLGEALRAALLAFNTFDES
                     ETVRHRQRMRVILQTPELQAYSMTMYAGWREVIAGFVARRLDGKTTDLLPQTVAWTML
                     GVALAAYEHWLGDESVPLPVALGDAFDVVGAGLERLNQ"
     gene            20876..21928
                     /locus_tag="VFI55_03465"
     CDS             20876..21928
                     /locus_tag="VFI55_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019256787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 family protein"
                     /protein_id="HET7073125.1"
                     /translation="MSARPEHLLHTLRAQGQFCSASGSPMYGELFELVAGDVETGGVF
                     ATILSGHEDDPSRHAVPLRLLGGLHRLVLDGRVPQLRRWYPSTGGSWDADAAWPEILR
                     TGAAHRNSLRAALEHPPQTNEVGRSAALIGGLLRVNREFDLPVRLFEIGSSAGLNLRA
                     DHYHYRYPGGQWGSVESPVTIDDAWHGALPPQGAVRIVERNGYDIAPIDVTGADGEMT
                     VLSYVWPDQDTRLRRLRGAVAVARNVPARLHRITAAAAVAGLRPAEGTLTVLWHSITW
                     QYLSDDERAAVRAGIDAVGAQADARSPFAHLTLEPARDGPGAPIRFLVRARSWPGGEL
                     AILGECHPHGPPVHWQ"
     gene            complement(21934..22911)
                     /locus_tag="VFI55_03470"
     CDS             complement(21934..22911)
                     /locus_tag="VFI55_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014710820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HET7073126.1"
                     /translation="MPRVAILDDYAGLALDLADWSPVQTRSEVTVFDRHLSEDEAAEA
                     LRPFDVICTLRERMALPRTLIERLPNLRLITIVGRSLPNLDMAAASECGVLVAHSDFA
                     NPRFSSVRDATPELAWGLLIATVRNLAEEHRRMRDGGWQVTTGMTLSGKTLGLLGLGR
                     IGKRMAEYAKVFGMQIIAWSHNLTEETAVSVGARRVDKATVFEESDVVSIHLVLSERT
                     RGLVGQTELALMKPHAYLINTSRGPIVDEAALIAALKTGRIAGAGIDVYDVEPPPPDH
                     PLRLLPNVTLSPHLGYVTREMLSAFYSDTIEAVVAWLDGAPIRIANPET"
     gene            complement(22916..24112)
                     /locus_tag="VFI55_03475"
     CDS             complement(22916..24112)
                     /locus_tag="VFI55_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7073127.1"
                     /translation="MTSSEAAERSEKGIVIVGGGLAAARTAEQLRRSQYTGRITIVSD
                     EVHLPYDRPPLSKEVLRKEVDDVALKPREWYDEKDIALRLGSAVVGLDTAERTITLDD
                     GTVLGYDELVIATGLVPRRIPAFPDLEGIRVLRSVDESMALREHASAARHAVVIGAGF
                     IGCEVAASLRSLGVDVVLVEPQPTPLASVLGEQIGELVARLHRGEGVDVRLGLGVAEV
                     RGQECVDTVVLTDGTELSADLVVVGIGSRPATEWLEGSGIEVDNGVICDEAGRTSAPN
                     VWALGDVASWRDATGHQARVEHWSNVADQARVVVPAMLGQDVPTNAVVPYFWSDQYDV
                     KIQCLGEPEADDIVHLVEDDGRKFLAYYERDGVLVGVVGGGMPGKVMKVRAKIAAATP
                     IAEVLG"
     gene            complement(24137..24964)
                     /locus_tag="VFI55_03480"
     CDS             complement(24137..24964)
                     /locus_tag="VFI55_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HET7073128.1"
                     /translation="MAGHAGSLQGRVAFVTGAARAQGRSHAVRLAREGADIVALDICA
                     PASHTLTYQPATPEDLAETVRLVEAEGRKVLAREADIRDDAALRHLVADAVEQFGRLD
                     IVVANAGVLGWGRVWELTDEQWDTVIGVNLTGTWRTLRAAIPAMIEAGNGGSIVVVSS
                     AAGLRATPGNGHYAASKYGLTALTNTLAIELGEYDIRVNSIHPYSVDTPMIEPGAMAQ
                     IFAKHPRYVHSFPPMPLQYKGFMTADEVSDVVVWLAGDGSGTVTGTQIPVDKGALKY"
     gene            complement(25162..25992)
                     /locus_tag="VFI55_03485"
     CDS             complement(25162..25992)
                     /locus_tag="VFI55_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HET7073129.1"
                     /translation="MLARYLKAQLVVLLCGGLVGPIFLVVYFTLGLDNVLQWMLYVGA
                     LITVADVVIAVALANYGAKSSAKTAALEQNGVLGLAQITGLTETGTWINNQQVVKVHL
                     HISGPGFLPFDSEDRVIASVTRLGNLNARKVVVLVNPTTQEYRIDWERSALVNGLMPA
                     QFTIAEDNRSYDLSGQAGPLMEILQILKANNVPLNRMVDIRSNPALRQQIQAVVRRAA
                     ERQAPAAAPAAPAGPAPVVTPPQQSIAQRLQELETLRAGGALTDQEYASRRAQIISEI
                     "
     gene            complement(26084..26911)
                     /locus_tag="VFI55_03490"
     CDS             complement(26084..26911)
                     /locus_tag="VFI55_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HET7073130.1"
                     /translation="MSFVFAAPEFVASAATDLANIGSTISSASAAALGPTSGVLAAGA
                     DEVSATVAALFEAHAQAYQALSAQAALFHQQFVQLMSGGAAQYAATEAANASPLQAVG
                     QELSSAIATPSQALAQSPAMSSGANGALTSGTGGTSGSGGFGTGGNGAAGRLVGVAAS
                     SVSPAAAATGGGGAFFGPGGAGFAGSLAAGESLGGAGFAGANGGLVGSGGVGAAAAEG
                     AEELGIGGAPLAAGSALPVTPLAAVPAAAPANSSATIRPAGPAYSPGTAQSAETAGE"
     gene            complement(27098..28288)
                     /gene="tuf"
                     /locus_tag="VFI55_03495"
     CDS             complement(27098..28288)
                     /gene="tuf"
                     /locus_tag="VFI55_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET7073131.1"
                     /translation="MAKAKFQRTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKFPDLN
                     EKKAFDQIDNAPEERQRGITINIAHVEYQTDKRHYAHVDAPGHADYIKNMITGAAQMD
                     GAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKADAVDDEELLELVELEVRELL
                     AAQEFDEEAPVVRVSALKALEGDAKWVESVEQLMDAVDESIPDPVRETDKPFLMPVED
                     VFTITGRGTVVTGRVERGRINVNEEVEIVGIKPTSTKTTVTGVEMFRKLLDQGQAGDN
                     VGLLLRGIKREDVERGQVVVKPGTTTPHTEFEGQVYILSKDEGGRHTPFFNNYRPQFY
                     FRTTDVTGVVTLPEGTEMVMPGDNTNINVKLIQPVAMDDGLRFAIREGGRTVGAGRVV
                     KIIK"
     gene            complement(28430..>29196)
                     /gene="fusA"
                     /locus_tag="VFI55_03500"
     CDS             complement(28430..>29196)
                     /gene="fusA"
                     /locus_tag="VFI55_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177746.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET7073132.1"
                     /translation="ETGQTIISGMGELHLEILVDRMRREFKVEANVGKPQVAYRETIR
                     RTVDKEEYTHKKQTGGSGQYAKVVVKLEPLAMTPDGPSYEFANEVTGGRVPREFIPSV
                     DAGAQEAMQYGVLAGYPLVNLKVTLLDGAFHEVDSSEMAFKIAGSQVLKKAAQQAQPV
                     ILEPIMAVEVTTPEDYMGDVIGDLNSRRGQIQAMEERAGARVVRAHVPLSEMFGYVGD
                     LRSKTQGRANYSMVFDSYAEVPANVSKEIIAKATGQ"
BASE COUNT         4709 a         9937 c         9517 g         5033 t
ORIGIN      
        1 aaccccgcca tcatcgaacc ggccgtcacc gccgcgatgg ccgacgtcaa tgcagtatcc
       61 gtcgccgcac agccggcggc cacggtatta ccacccatgg ggtccggttc cgggaatggc
      121 acgtggctgc ccatcgggtc cgccggcctg ggcgtcggag gcgtgccggg cgcagacctg
      181 agcggggttt cggcgggcat cagccgggcg gcgttcgttg ggcgattgtc agtaccccaa
      241 agctggaccg aggccgtttc ggtggtgaac cattcgggtg cggcgctgcc gggaggcggc
      301 tggatcggca cggccgtccc ggacagctcg gtaggcatgc ccggtatgcc ggggatgccc
      361 atggcccgca ccgccgggta cagcttcggg catgggcctc gatacggatt ccagccgacg
      421 gtgatgccac gtccacctgc tgccggttga tggaaacgcg tcttccctcc gcgcggctgt
      481 taaccatttc acctgatgtt tgtttgcgtg ttagttcgtt tgctgtaaat gtcgatttgt
      541 gtcgaaatcg gggtgacgtg tgcgtgctcc ccgacaccga gctcgccgcc gacgccgggc
      601 gccatcgtgg ccgaacgagg ccacgcgccg caatgtgccc gacatctaca ccgttggagg
      661 ctgagtgcac acccccccat cacctacccg ggctgaattg gcgacacggc gtccgttttt
      721 caaggagaca atcaaggtga agcacagtcg atcagccgcc gcgatgagct tgctgactgc
      781 ggccgctctg gcgctgtcgt catgcagtaa ctcccatacc accctggcct atgcggccgg
      841 ggccaaggtg gactgcggcg gcaagcaaac cttggcggcc agcgggtcga ccgcgcaggc
      901 aaacgcaatg acgaaattca tcgacgccta ccacattgcc tgctccgggc agaccctcag
      961 ctacaccgcc aacggctcgg gcgccgggat cagcgacttc ctcgccggca agaccgattt
     1021 cggcggatcg gacacaccac tgagcgggga ccaatacacc gccgccaaac agcgatgcgg
     1081 cggcgccgac gcctggaacc tacccgtggt gttcggcccg atggccatca cttacaacct
     1141 cagcgccgtc gattcgttgg tgctcgacgc gcccacgctg gccaagatct tcaacggcac
     1201 catcacgcgc tgggacgacc cggcgctcac cgcgctcaac gcatccatgc ctgcagagga
     1261 catccgtgtc atctaccgca gcgacgcgtc gggtacgacc tacaactttc agtcctacct
     1321 acaggccgcg tccggcggag cctggaatca gggcgcggcc aagacgttca acggcggcgt
     1381 cggcaccggg gccgcgggca acagcggcac agccgcactg gtgaaaacca ccgagggtgc
     1441 catcagttac aacgaattgt cgttcgcgct gcagcaaggg ctgtacgccg ccgagatcaa
     1501 gaccccggcg agccgcaggt cgttacgtcc ggtacggatc ggcaccgaca cggtcggcaa
     1561 gaccatcacc ggcgccaaga tcaccggcag cggcaacgac ctggtgctcg acatttcgtc
     1621 gttctacaac cccgcccagc ccgacgtcta cccgatcgtg atggccacct atgagatcgt
     1681 ctgctcgaaa tacccgaatg ccgacgtggc caaggccgtc aaggccttct tgcaggcggc
     1741 gatcgggccc ggccaggtcg atctgaacag aatcggctac atcccgctgt cgcccgactt
     1801 ccaagccaga gtctcgaacg ccgtcgacgc catcagcgct gcggggacaa cgaattcgga
     1861 gtagtaccgc tcgacagccg cagcccgccc accccgccga cggccgcgca ctcagctcgc
     1921 gccgccggtt tagtcgcgcg aaaacgcaag cgcgagttct ctttccacgt cttcataggg
     1981 cctgcccgac acgtcgacgg tgacctgggc gatggtgccg ccggtgaacg cgaacggcgc
     2041 cttgtagcgg ctcgacactc ccgaaccgcc gttgcgcccg acagtgatgc cggcccccgc
     2101 caacccgaaa gttccggggt gggcggtcac gccggcaagg gaaccgacca aggtgtcgtc
     2161 gatgaacagc gtgacctccc cgactggggt gtggctgttt tcgaccgttc ccgtccgctc
     2221 gtagcgcaca ccgaacacgt ggcggcccag cggcaccggg ccagacgaag agatcagctg
     2281 ctgacgctcg ccgaggaagt tgtacacgta gtgcagtcgc ccgtcctgga tgaacaagac
     2341 atgtccgccg tggccaccgc cctgcttgaa caacacccct tcggcgccgg tggtatccac
     2401 ggtcacgtcg gcgagcacag cgaacgagcg cccgcgaatc tcgacggcgg cgccgatgcc
     2461 gacgtccgcg cagtcgggat agtagatgta gctgtcccgt tcgccgatca ggtagggccg
     2521 gaacctggtg agcgtttcca ggaggttcag atccgccagc ggcaacccgt tgtacttggc
     2581 ggcctcggaa aaccacagcg ccttgagttc ttcgagtttg tccggcatct cggcggccag
     2641 gtcgtgacac tggctgcggt ccgcctcgat gtgaaacagc tcccaacgat cggcgtcgaa
     2701 atgcgaccaa cccgccgggg ttgccgcgtg caccgtgttg gcgaaccagc ccttgtgcca
     2761 aatcccgcgg gtacccagca tggtgtagaa ctgagtttcc ttgccggtgt cggtgctcga
     2821 atcggcaagg gccgctttga aactgatgcc gtccagcggc gtctgcgtga tgcccttgac
     2881 ggtctcgggt gccgtcatat ccagcaggtc gtacacggtc ggagtgatgt cgcagacgtt
     2941 gacatagttg tcacgcacct cgccgtgcgc cgcaatgccg ttgggccagg agatgattgc
     3001 cgtgtcggca atgccgcctt cgtgcgaggc gtagcgcttg tagagcttgt agggcgtgtt
     3061 gaaggccatc gcccagccga tcgggtaatg gttgtaggtc tccggcccgc ccaggtggtc
     3121 gaacagcttc atgctctctt cgacagtgtc gatgtagccg ttgaagaact tgccttcgtt
     3181 caccgatccg ttgggcccgc cctcaccact ggcgccgttg tcggaaatca ccacgatgat
     3241 ggtgttgtcc agctggccgg actcctcgag atagtccagg atgcggccga tctgggcatc
     3301 cgtgtagctc aggaagccgg caaaaacctc ggccatccgg ctgaacaggt tcttctcttc
     3361 gtcgttgagt gagtcccacg gccgcaccgt atcctgcagc ggccagggct cgccctgcgg
     3421 tcccttgacg tccaaatacg ggttgacggg cgacaattcg gtgtccggcg gcacgatccc
     3481 cagggacttc tggttttcca gcacaatctc gcgatagcgc tcgtagccca tgtcgaaccg
     3541 gcccgcgtac ttgtccgccc agtccttgaa cacgtggtgc ggcgcatgcc cggcgcctgg
     3601 gcacacatag ctgaaccacg gcttgtcggg cgcgatcacc ttggcatcac ggatgaattc
     3661 gatggtcttg tcggcgatgt ccttcgacag gtggtagccg tcttccggtg tacccggcgg
     3721 gtttacgggg tgattgtcgt acaccaggtc gggataccac tggtcagtct cgccgcccat
     3781 gaaaccgtag aagcgttcga agccgcgcga cgtcggccag tgccgtttgg tagacgccag
     3841 attcgactcc tcgaggggcg tcaggtgcca cttgccgacg cagtaagtgt tgtaaccacg
     3901 ttcggcgagc acttcggaga gcagcgcggt atcggccggg attcgtccat tgcagttagg
     3961 gaacccgtcg gtgaactctt cgatggtggc catgccgacg gtggtggcgt tgcgtccggt
     4021 cagcaaggac gcccgggtgg gcgaacacag ggcggtggtg tgaaattgcg acagtcgcac
     4081 gccacgtgcg gctatccgcg tcatggcggg catctcgacg aggccaccga agcagtccca
     4141 cgtcgcgatc ccggtgtcat cccagaccaa atagaggatg ttcggtgagt tcgcgggtgc
     4201 cgtgggcgcg gcgtacggac cccagtccgg ttccgagtcg cgaatatcca gcgcgatctt
     4261 gcccgtgaat tctgtcgcca tcgtcagcct tacttccgtg gtggacctac cgagggtcgc
     4321 gtgcagacta caccggcgac atggcgactg gccgaaagtc cagcacggcg gcggggccaa
     4381 gcggcgccca tctggggcac cgcccgcttg cgagcgtaac gccaccgcga aatcgccggc
     4441 cgattttcgc agcggcgtta cgctcgccga actgcaatcg cagaatcgcc cgggacctcg
     4501 taaggtcccg ggcgattccg ttgcgacttt gcggctagtt ggccgttact tggccctctc
     4561 caaaatctcg acgagccgcc agcgcttggt ggccgacatc gggcgcgtct ccatcagcga
     4621 gacacggtca ccgatgccgg cgacgctgtt ctcgtcgtgt gccttgacct tcttggtggt
     4681 ccggatgatc ttgccgtaca aggggtgacg catgcggtct tccagctcga ccacgatggt
     4741 cttctgcatc ttgtcgctca ccacataacc gatgcgcacc ttgcgccggc cgcgcgctgt
     4801 cggcgtgggc ggagtgtgct tggggccctt gtctttcgat ccggcggctt ccggggccgc
     4861 cttcggggcg gacttggccg ccgaggacgc aaccttcgct tctgccatca cgcttccttg
     4921 tttccgtcgg acccgttggg tccgctagcc aggcccagtt ctcgttcacg caacacggtg
     4981 tagacgcgcg cgatctcgtg acgcaccacg cgcagccgac ggttgttggt cagctgtccg
     5041 gtcgccatct ggaagcgcag gttgaacagc tcttccttgg attcacgcac gcgctcggcc
     5101 aactcctcgt cggtcagctc gcgcagttcg ccaggcgaaa ttccgactgc catcagaact
     5161 gctcctctcg ggtcacgatg cgtgccttga tcggcagctt gtggatcgcc cgggtcaggg
     5221 cagcacgggc gatctgctcg ttggggtagc tgagttcgaa cagcacccga cccggcttga
     5281 cgttgacgac ccaccattcc ggggagccct tacccgaacc catccgggtt tcggcgggct
     5341 tcttggtcag cgggcggtcg gggaagacgt tgatccacac cttgccgcca cgcttgatgt
     5401 ggcggttgat ggcgatacgc gccgactcga tctgccggtt cgtgacgtag gcgtgctcca
     5461 gggcctggat gccgtaatca ccgaagttca ccgacgtacc accgctggcg ataccgcgct
     5521 ggcggggatg gtgctgctta cggtgcttga ctttgcgtgg aatcaacatg attcagctct
     5581 ccgtgctctc cgtgctctcc gtgctctgcg gttccaagga ggacacagtg ggcgcagcgt
     5641 cgccggatgt cgtgctctct tcggcgccgg cggcccgccc ggcctcggtg ctggtcgcgg
     5701 tggtgcccga cgcgccgctg cgccgtggac gcgtacccga cgggcgctcg cggcgcggac
     5761 gctcggcgcc cgccggtgcg gcgaccgcca attcgcgttt gcctccgacg atgtcgccct
     5821 tgtagatcca caccttcacg ccgatccggc cgaaggtggt cttggcctca tacaggccgt
     5881 agtcgatgtc ggcgcgcagc gtgtgcagcg gcacccgtcc ctcgcggtag aactccgagc
     5941 ggctcatctc ggcaccgccg aggcggcccg agcactgcac ccggatgccc ttgacgttgg
     6001 gctggcgcat ggccgactgg atcgccttgc gcatcgcgcg gcggaacgcc acccggttgc
     6061 tcagctgctc ggccaccccc tgcgccacca attgtgcctg cgactccggg tttttgacct
     6121 cgaggatgtt cagctggacc tgcttcttgg tcagcttttc caggtcggca cgaatccggt
     6181 cggcctcggt gccgcggcgc ccgatgacga tccccggacg cgcggtgtgg atgtccaccc
     6241 ggacccggtc tcgggtgcgc tcgatctcca cgtcggcgat cccggcgcgc tccaggccgc
     6301 tggacagcag ccgtcggatc gccacgtctt ccttgacgta ctcggcgtac tgcttgtcgg
     6361 cataccagcg ggacttccag tccgtggtga tgcccagccg gaagccatgc ggattgatct
     6421 tttggcccac tagtctgagc cccccttcgc ttccgaagtc tgcgcggttg ccgtagcagt
     6481 tccagtagtt ttgccggttg ccttagcctc agcggatttg gctggtgctt tcttggccgc
     6541 cgtcttgttc gccggcgcct tcttcgccgc ggccttgctg gcctcggcgc ggcgggcccg
     6601 ggtcgacttc gccgaaccct gatccttggc cggccgactc tcgaccacga ccgtgatgtg
     6661 gctggtgcgc ctgcggatcc ggaacgcgcg gccctgcgcg cgcggacgga tgcgcttggc
     6721 ggtcggtccc tcgtcggcgt acaccgtcgc gaccaccaag gtcgcggggt ccagcccatt
     6781 gttgttctgg gcgttggccg cggcactggc gatcaccttg gccaccggct cgctggcggc
     6841 ctgcggcgcc cagcgcagga tgtcgagcgc gtcggtcacc gagcgaccac gcaccaggtt
     6901 gatgacccgg cgcgccttgg tcggcgagac ccgcacgaac cgtgcgacag ccgtcgccga
     6961 cgggaattca gtagtggtgg tgctcatcgc cgtttggcct tccggtcgtc cttgatgtgc
     7021 cccttgaagg tgcgcgtcgg cgcgaattcg ccaagcttgt ggccgaccat cgcctcggtg
     7081 acgaacaccg gaacgtgctt gcggccgtcg tgcaccgcaa aggtgtggcc gatgaagtcc
     7141 ggaatgatcg tcgaacggcg cgaccaggtc ttgatgacct gcttgctgtt cttctcgttc
     7201 tgaacgtcga ccttcttcag caggtggtcg tcgacgaacg ggcccttctt caggctgcgt
     7261 ggcatgtgct actcctacgt ccgattgccc ggcttctcct catcgctgcg ctctgcatcg
     7321 tcgccggcct accgcgagtg cttcttgccg gtgcgccggc gtcggacgat gagcttgttg
     7381 ctgagcttgt tcggatggcg ggtgcggccc tcgggcttgc cccacgggct gaccgggtga
     7441 cggccacccg aggtcttgcc ctcgccaccg ccgtgcgggt ggtccaccgg gttcatgacc
     7501 acaccacgga cggacgggcg cttgcccttc caccgcatgc gaccggcctt gccccagttg
     7561 atgttcgcct gctcggcgtt gcccacctcg ccgaccgttg cgcgacagcg cacgtcgacg
     7621 cggcggatct caccgctggg catgcgcagc gacgcgtagg tgccctcttt accgagcagc
     7681 tggatgctcg accctgccga acgcgcgagc ttggcaccac caccgggccg cagttcgacg
     7741 gcgtggatca acgtaccggc cgggatgtta cgcagcggca ggttgttgcc cggcttgatg
     7801 tcggcgttgg cgcccgactc caccacgtcg ccctgcgaaa gcccctgcgg ggcaatgatg
     7861 tagcgcttct cgccgtccag gaaatgaagc aacgcgatgt tggcggtgcg gttggggtcg
     7921 tactcgatgt gtgcgacctt ggcattgaca ccgtctttgt cgttgcgccg gaaatcaatc
     7981 acccggtagg cacgcttgtg gccgccaccc tggtgacggg tggtgatgcg gccgtgtgcg
     8041 ttgcgcccac catgcccgtg cagagggcgc accaacgact tctccggctg cgcgcgggtg
     8101 atctcggcga agtcggacac gctggcgccg cgacgcccgg gggtcgtcgg cttgtacttg
     8161 cgaatagcca tgtctaatca ggtctttctc tcgcgcggct aggccggcgc tccgaacagg
     8221 tcaatcggct tgctgcccgg cgccagggtg acgatggcgc gcttggagtt cttgcgcttg
     8281 ccgaagccgg tccgggtgcg cttgcgcttg ccctgccggt tggcggtgtt caccgatgcg
     8341 accttgacgg aaaagatctt ctcgacagcg atcttgatct gcgtcttgtt cgaatcgggg
     8401 tgaaccacga acgtgtacac gttgtcgtcg agcagcccgt aggacttctc cgaaatgacc
     8461 ggcgccagga tgatgtcgcg ggggtcagcg atggtcgcca tcaggccgaa acctcctcgg
     8521 tgctcgccgt atcggccacc gtgtcggtgg ccgtgttcgc cgcgatgtaa gcgttgagcg
     8581 cctcgacgct gaacacgacg tcatcggacc gcagcacgtc atacgtgttg agctgatcgg
     8641 gcgtcaggac gtgcacaccc ggcaggttgc gcacgctctt cgtgccggtc tcgtcggtgc
     8701 gcccgatgat taacagcact tgcttgcggt cggtcaaggc gtccagaaat gccttggcgc
     8761 tcttggtgga aggggtctgg cccgcgacca gctcagtgac cgcgtgaatg cgcccgttgc
     8821 gtgcccggtc ggacagcgcc ccgcgcaacg cggcggcgat catcttcttg ggcgtgcgct
     8881 ggctgtagtc gcgcggcttg ggaccgtgca cgacgccgcc gccggtgaac tgcggggcgc
     8941 gcgtcgagcc ctgccgggcc cgaccagtcc ccttctgccg gtagggcttc ttgccgccgc
     9001 cgcggacgtc gccacgggtc ttggtcgagt gcgtgccctg gcgggcggcc gcgcgctgag
     9061 cggtgaccac ctggtgcatc agtgcgatgt tggccggagc gtcgaacaac tcggccggca
     9121 gctcgatgaa gccgtcgacc ttgccgcccg gcgtcttgac ctgaattttg aggcccttct
     9181 ggtcctcttg agctgccatt acttctcacc tcgtttgatc gcgctgcgga ccatcacgag
     9241 tccaccggtg cggccgggga ccgcgccctt gatcagcagc acgccgttct cggtatcgac
     9301 cttgtgtacc aacaggttct gaacggtcac ccggtcgttg cccatccggc cggccatccg
     9361 ggtgcccttg aagacccgcg ccggggtggc gcagccgccg atggagccgg gacggcggtg
     9421 caccgcctgg gcgccgtgac tggcgccctg gccgcggaag ccgtgccgct tcatggtgcc
     9481 ggcgaagccc ttgcccttgg acgtgcccgt cacgtcgaca tagttgccgt cggcgaagat
     9541 ctcagccgtc aactcctgcc cgacctcgta ctcggccgcc gcgtccgggt tgtccaggcg
     9601 cagttcggcc agataccggc gcgggttgac gcccgcggcg gtgtactgac cggtcacggg
     9661 cttgttgacc ttgcgcgggc tgatctcgcc gtaagccaac tgaacggcgc tgtagccgtc
     9721 gcgctccggg gtgcgaatgc gggtcaccac gttcggcccc gccttgacca ccgtcaccgg
     9781 cacgatccgg ttgttctcgt cgaatacctg cgtcatgccc agcttggtac ccagaatgcc
     9841 tattctcgcc attgctctgg aatctcctac tggatgttga cgtcgacact ggccggaaga
     9901 tcgatgcgca tcagcgcgtc aaccgtcttc ggcgtgggat cgaggatgtc aatcagccgc
     9961 ttgtgagtgc gcatttcgaa gtgctcccgc gagtccttgt acttgtgcgg agagcggatg
    10021 acgcagtaga tgttcttctc agtcggcagc ggcaccggac ctacgacgct ggcacccgtg
    10081 cggacgacgg tctcgacgat cttgcgcgcc gacgcgtcga tggcctcatg gtcgtaggcc
    10141 ttaagcctga tgcggatctt ctgtcccgcc acgcttctcc taccctcact cctgttgagg
    10201 cccgtccccg ggcctggtgc cccccagcgc gccaatcggc ccggccaatg ttggcccggt
    10261 cgagcgtcgc gccggtactg cgccgctgtt tacctgtcgt ggtccaccga cccccgcggt
    10321 cgggtgtgtc acccttacgc agccttgttc gcggccgaaa ttcgtcgctc gccaaggatg
    10381 ggaccggacg cgcccgttta ggcgctggtc gtatgcccgg ccaggtacag acctggcgca
    10441 aggcaacctg aacagtatgc ctcagatcat gggtttggcc aaatctcggg caaacatcgc
    10501 cagcatggtc gcggacacgg tttcgcagga cccctaaccc ggtggtcagg ggtcttcttg
    10561 aggtacagac ccaccctcgc tcagactggc acccgttgag gaaattccgt gatcgcgagg
    10621 cgaggcaggc gatgggcaca ccgacggtcg acccggtatc gcgcgaggcc gccaaggtgt
    10681 tcgccgaccc aaccgcctac gcggacgagc cgcggctgca cgcggcgctg acccatctgc
    10741 gggcccacgc accggtggtg ccggtcgact gtgcgcccta ccggccgttc tgggcgatca
    10801 ccaagcacgc ggacatcatg gagatcgagc gcgccaacaa tctgttcatc aatgaaccga
    10861 ggccgctgct cggcacggcc gccgccgacg acgtcgcgcg ggctcagctg gacgccggga
    10921 tggggctgcg cacgctaatc catatggacg atccgcaaca tcgggtggta cgcgcgatcg
    10981 gcgccgactg gtttcggccc aaggcgatgc gggcgctcaa agtccggact gacgaactcg
    11041 ccaaacttta tgtcgacaag atgatggcgg cgggcaacga atgcgacttc gtgcaggaag
    11101 tcgcggtgaa ttaccccttg tacgtgatca tgtcgctgct gggcctgccg gagtcggact
    11161 tcgggcgcat gctcaagcta acccaggaac tgttcggcgg cgacgatgcc gaatttcgcc
    11221 gcggcaccag cctcgacgag caactccagg ttctgctcga ctttttcgga tacttcaatg
    11281 cgctcaccgg atcgcgccgc gagactccga ccgaagacct ggcctcggcg atcgccaacg
    11341 cccgcgtcga cggcgagccg ttgtcggacg tcgacaccgc gtcctactac gtgatcatcg
    11401 ccaccgccgg ccacgacacc acgagcgcga cgatcgccgg aggcctgcgc gcgctgatcg
    11461 aaaaccccga ccaacgggag cgactgacct ccaatctcga cttgatgccg ctggccaccg
    11521 aggaaatgat ccgctgggtg acgccggtca aggaattcat gcgcaccgcc actaaggaca
    11581 ccaccatccg cggggtgccg atcgccgaag gcgaagccgt atatctggct tacgtctcgg
    11641 gcaaccgcga cgaggagatg ttcgacgatc cgttcagttt tgacgtcgga cgagacccca
    11701 acaagcactt ggcattcgga tacggcgtcc acttctgcct cggggccgca ctggcccgca
    11761 tagaggtgaa cagctttttc agcgaactgt tgccgcgact gaaatcgatt gagctcaatg
    11821 gcgaacccga actcatcgcc accacgttcg tcggaggcct gaagcacctg cctattcgat
    11881 attcgctgcg ctagccgttt tcgcgctgtc cggcgcaacc ttcgaattcg tccgtctcgg
    11941 caggccgtag ttgcagatca cgggtcatcc ccgctgatga cggccggcgc tgggcatttc
    12001 ctcggcactg tcattctggt acgtaccaaa actttcggct ggtgctattc gagggcacgt
    12061 tgttgatatc ggttggcttc gcgtgcatgc caatacttga cctccattcc gcattgcgcg
    12121 aattaacctg gttcactttg atattcagca cggccgtcgg gttctgcacc gcacctacga
    12181 accgcggaga tcctgagtgc ggttcgggat tagaaatagg gcgccgccta agcggtgggc
    12241 atcgccgcgg ccggcgggct gcaccggacc ggtttgcagc gcggcaaccg actcgccgcc
    12301 acggcgatgg ccgaggtcag caaatcagca ttcctcacca ccacgcacgc ttcgatggcc
    12361 gtgatggccg taatcgtcgc ggtctccgca ctccggatcg ggcggtgggc cccaggacgt
    12421 gacgtgcaac agctaatcct ggtgcgccgg gcctcgacgg ccgcaggtag ccgcgccgaa
    12481 cccgcctact gaagttcacc tgaaaacgcc tcggcggatg cggtggtcgg gttaccatcc
    12541 ggatgagcgg agttcgccct ggggagggcg cgcgaactcc aggcaggtgg ggagcgggat
    12601 gtcgtacgtg atcgcggcgc cggagtatgt ggccgctgcc gcaacagatt tggcgaatat
    12661 cggatccacg atcagcgatg ccaactccgt agcgtcgggg ccggtgtcga gcatattgcc
    12721 cgccggagct gacgaggtgt cggcgagcat tgcggcgctg ttcgacgcgc acgcccaggc
    12781 ctatcaggcg ctcagcgcgg cggcacaggc gttccacacc cagtttgtcc agctgatgag
    12841 tgccggctcc gagcaatatg cgctcaccga ggccgcgaac agcacgccgc tgcaggccgc
    12901 cggccagggt gtgctcagcg gggggcacgc ccccagtcag gtcttgaaag ccgcaaccac
    12961 ggtgcagccg gtgatgggca gcgcggcatc ggcgggttcg gcggcggcgg gggtaccggc
    13021 ggctggggca tccgcgacgg gggtggcggg tgggtcggtg ccggccgcac cggcggcacc
    13081 cctgacggcg gcgatcgcgc ccatatcggc tccgccggcc gggctggcat ccacgagttc
    13141 gccggccgct gccgtgccgg cggggtcggc tgccacgccg gtatcgccgc tgcaggcggc
    13201 cacaccggct tatgctccgg caaccgcgga tgcggtggcg gcaacaccgg tcggcacgcc
    13261 ggtagcggcg gtgccggccg aaacgcccga actctctccg gcggcggcaa ccgcgttgcc
    13321 cgccgccacg ccggtgacgg cggcgcctgc ggcaacggcg gcttattccc cggcaacgcc
    13381 accctattct ccggcgacgg ccgcgtcggg atcgccgaga gagcagtcgg ccgcacccgc
    13441 ggagtacggc gaaatgacgc cctactcagc ctaattcagc cgaacgaacc cggccgcgcg
    13501 atgtccaagc atcacaatgg tggcgtgcgg cccgcggctg gtgatcgaag gcaggatgga
    13561 accgtctatc acccaaaggt tttcgatgcc gcgcacccgg cagcgatgat cgacgacggc
    13621 tcgcgggtcg ccgtcggctc ccatcgggac gctggcacac aggtgctgcg acgtcgacca
    13681 caccggagac ccgacatgtg tttccgcacc ggctaattcg cgggccaatt cactgccccg
    13741 gcgcagcgct gcgacatcgt cgggctcgct gtcgtagcga tgctcgatac gcggcggcac
    13801 accgggatcc cgcgacacga gcatgacgcg cccacgcgcc cgtggctgca tgagcgccac
    13861 tccgatgtgc ggccagtcgg gatgccccgg ggtgccgtca ccggtcatgg cgacgaatcc
    13921 gcccgtgtac ggccggatct cgatcccgtc gtcggtgctc agcacgacct ctatcaccgg
    13981 ccgcccggta gcgacggccc agttggtcgg caacacccac tcggggtgat cgctgcagaa
    14041 catgcccacc ggcagcggcg ataccacggg cacgccggcc gcacgcaaca ttgcttcatc
    14101 gccgatgccg gacaacatca gcagatgagc agactgaatc gccccggcac acaacacgat
    14161 gcgggtagcg gtaaacgtcg tcgggccctg cgggccgatc gcgtcgacac cgaacgcggt
    14221 gctgccggca aaacgcactc gcaccgcccg ggtttgcgcc agcaacgtca ggtttgatct
    14281 gtccaaggcc ggaatcagat atgccgcgcc ggacccgatg cgcacaccgt cgacgatgtt
    14341 gagtggtacg gcgcccacgc cagaggtcag ctccggcccg caatcgttga ggtcggcaat
    14401 ccagggaaaa ccggcacgcc cggcggccgc gatgaaccgt tcggtggtgc cggtcatgtc
    14461 gcgagttcgg cgaaccagga tggggccgct gtcgccgtgc gcgggaccat cgaaatccaa
    14521 gtcggtctcg atcgcgcgga agtggtcgag aacatcagac cacgcccagc cggggattgc
    14581 ggcgcgatcg aagtcgcgcg gcattccgcg gcagaaatac ccgccattga cggcacccga
    14641 tccgccgacc gtcgatccgc gcacgatggg cagctgcaaa gcgtggcggt cggtgagccg
    14701 ggtcaggtag cgctgggcca gcgggctgcc caccccgatc ggcagttgca aaccgttggt
    14761 cgtccgagcc agcaactccg gatcagtcaa ccctggcccc gcctccagga cggtcaccac
    14821 gcaggcgggg tcggcggaaa gccgttcggc aacaaccgat ccagcgctgc cggcaccgat
    14881 gatcagcaca tcgctgtgca acatggcttc ggtcaaggca acgccaaggc ggccgttaac
    14941 tccgaaactg cggtttgagc gcgcgcaggt tgcgttcgcg caccacgccc caccacaggc
    15001 ccaggccgta ggccaggtcg tcgacgcgct tgagcagcag gtaggtcagc agcccgatcg
    15061 gctcgatttc atcgctgctg gcgtcccggc gccgaagcca gtccaccacg ccgtccatga
    15121 cagccgcgac cagcacgacc cgtcggaagt ggcgcgacag gatggccgcg aacaacgcca
    15181 gtggccagta gtgccggcat agggccgacg cgagctggag cgccgccgac cccaaaccgc
    15241 ggctggcgat cagcagaacg tcggtcatcg aagtctccgc gctgcgcatg gccctggcga
    15301 tccgccggcc cgtcaacagc gcgatcacga tcgaggtgag ttggctcagg ctcgtcccca
    15361 gcgccatcag aatccaggcc atcaaggccc agccggagat caccaccggc gccgtcttgt
    15421 ccgggtggcg cacggacagc ggagccgccg aacccccgta gaacgccttg cgcgcaagcc
    15481 agtcccggag ctcggtgcgg tgatcgtggg cgaccagtgc aatcggctcg taccgcagcc
    15541 gggcgcccgc ttcgatcagc cgccagcaca ggtcgacgtc ctcgcccgac tgcatggtct
    15601 cgtcgaaccc gccgacgtcg cggatggccg agcaccggca gatgatcgcc gcgctgggaa
    15661 cgtaggacac cgtgctgtgg ggtaacaccg gcgcctcgcg ctgcccaagg tccagcgacg
    15721 agtgcaccgc ctcatagcgc gcgaccacat tctcgctctg cgagagaccg acgatgcgtg
    15781 gcgcgaccaa ggcgacggtg gggtcgcaaa agtgacccag gagcgcctcc agccaacccc
    15841 ggcgcggcgc gacgtcggag tcgaggaacg cgacgaagtc ggtcttgcaa gaggccagcc
    15901 ccgtgttgcg tgccgccgcc ggacccttgc tgaacgtgtg gcgcaacacc tcgacgtcgc
    15961 agtgcgcgcc gacgaagtcc gccggctcga tcggcgggga cgaaccgtcg tccaccacga
    16021 cgacacgcaa tccgcgcagc gagctcacca ggcgccgaac accagaaacg ttgttgcgta
    16081 ccgggattac gacggtcacg tcgcggtgcg atgggccacc cgagggcctg ggatgggcca
    16141 ccgtggcgtc cagcagggtg cgggccagtt gggcgctgac gtcgtcgcga accttgagcc
    16201 ggccatcgga cagcatgtcc tgggcggcgg gagccagctt gagcaggcgg gtcggtgagc
    16261 cgccgagcag ggccgagccg tcgccgagta cccgcactcg gcgatcgacc tggacagcga
    16321 aaccgtccgg cagtcggggt tggctcatgt cagcatcccg tcgggcccgg gcagccaccg
    16381 ggcgactcga cggacacacc cgtccaccat ctcgccgaag atgcggctgc cctcgacggc
    16441 ggtcgcggtg gtcgggtccc ccagcacgcc gatccggctg accgccgcga ctcccccgcg
    16501 acgcatcggt ccgatcaatt ccgccaacgg tgcccggttt ccggcgagcc agcgatcgct
    16561 gcgcacctcg gccggcgaga tatgcagcaa cagcgatgtc tcgctgtggc cggcatgggc
    16621 gtcgccgccg gaggcagcgc aggcgctcca tccgacgtcc cggccttcgg cgcgcaacgt
    16681 gcgcaccgcg ccgcgcagag cgccgacgtt gccgccgtga ccgttgacga agaccaggcg
    16741 ctgggcccag caggcggccg acctgccgta ctccaccagc agcgtcgtca gggcatcggt
    16801 accgatcgag atcgtgccgg cgaagtcctg gtgctcgccg ctggcgccgt aggcgatggc
    16861 aggtgccacc aaccactcgt gcccgagcag ggttcggaga ccggccgcag ccccgcgggc
    16921 gacggcggtc gcgatccggg tgtcggtgtc caacggaaga tggggaccgt gttgctcggt
    16981 cgaacccaat gggaccatta tggacggcga ggtgctggaa agctggctcg aagtcgccgt
    17041 tcctaattcg ccgagtacgg gcactggctg atggtaggac gaattcacct ggcgtgcacc
    17101 aattgttgta ccttcaaaac aaattttttc tgagccgttc atcggttggg cggaattcaa
    17161 gactcgtttg tcacgggtac cccgcgagca ccacaaattt tcgcgtccgt cgattgtaag
    17221 caaagggcat tcccgcgact gccgaaaata gcgcaccgct aatccaggca aagctgccca
    17281 acatgaccgg aaatttgccg gcgcccccgc agcgacccgg ctaggcatcc gcggcggacg
    17341 ccgcaccgag cgtccgggtg aagccgggcg gcaccaggac gtcgtcgggg ctcaggtcat
    17401 ggaccgagga gtgacccaga cccatcagcg ccgagtcgac gccggcgcgc aagatgtcga
    17461 ggacgttttc gacgcccgcc tggccggccg cgccgaggcc ccacaggtac gcccggccga
    17521 tcatcaccgc gcgtgcgccc agcgccaccg ccttgacgac gtcgccgccg cgccggatgc
    17581 caccgtccag cagcacctcg acctggtcgc cgaccgccgc ggccaccgcg ggcaacgcgc
    17641 gaatcgacgc cggggtgccg tccaggttgt tgccgccgtg gttcgacacc gatatcgcgg
    17701 aaacaccggc atccactgcc cttttagcgt catccacccg catcacgccc ttgagcatga
    17761 acggtccgcc ccacagctcg cgaagccacg cgatgtcttc ccaggtcggc ggaggcgtgc
    17821 ccatccactc cccgtatgcg gcgaagaagg gcgggccggc ctcgccgcgg cgaccctggt
    17881 tgggcacccg cagctgcggc ggccgcagtg tcttgccgaa cttccacagc caccgcggcc
    17941 ggaggatcgc ctccggagac aaccgcagca cggtccgcag attcatcgcc tcggggatct
    18001 ccgggctccc ccagtcccgg ccgtgcgaga acgtccagtc cgtggtgacg atcagaccga
    18061 ccgcgccggc ctgacgcgcg cgttccaccc gctcggcgat cgcgtcgcgc ccgccgagcc
    18121 agtacacctg gaagaaaacc ttggggttgg cggcgatgac ctcctcgatc ggcttgctgg
    18181 cgaaagagga cagccccatg gccgttccgc gtgccgcggc ggcccgcgcc acggccacct
    18241 caccgtctgg gtcaaccgcc tgaacgccgg tcggcgagat gatcaccgga agcgcaagat
    18301 cctgtcccat aacggtagtt gacagatcac gctttaccga cgcgccgatc acgtgcggcg
    18361 cgaagcctag ctcgctgaag gctgcgacgt tgtcggccac cgtaatgcct ttttcgctgg
    18421 ccgaaatcaa cgacgagtaa acggatttcg gtagccgtcg cttcgcgcgc tgttgcgcga
    18481 cggcgaccgt ttcgaaccac tcgttggcca tcactacagc gggctttcgt tgcacggccg
    18541 cgccggcggc cgcacggaga gggtcagcgc caccggttgg cggcccggct gcccgtgcga
    18601 gtggtcagcg cgcggccggg gcgtctggcg ctcacgggac agcgccgacg cactgtgccc
    18661 ctggacgcac tcgggatccg ggccgtcgat cggcaggccg gtgaagaact tggccgccat
    18721 gcagcctccc cggcagctgt cgtagtgccc gcaggtgccg cacgcgcccg ccgactgcgg
    18781 ctcccgcagc tcacggaaca gcggcgcgtt cctccagacg ttgtcaaatc ccccgtcaga
    18841 cagaatgttt ccggccaaga agcggtcgtg gatggcgaac gggcaggcgt acacgtcgcc
    18901 gaccgggtcg atcaggcaca ccacgcgacc ggctccgcac atgttcaggc cggccagtgc
    18961 accggactgg ccgagcggcg acagatggaa gaaggagtcg ccggtgagca cccgctcccc
    19021 cttggcgacc agccagtcgt agagcgcaac ctgttgggcg gcggtggggt gcagatcgtc
    19081 ccacacgtcg gcgccgcgac cggagggacg cagccgggtg atgcgcaggg tcgcaccgta
    19141 gcggtgcgcc aaggccgcga attcgtcgag ttgcccgacg ttgtgccggg tgaccacgac
    19201 cgagatcttg gcgccgcccc cgctgtcggc gaaccccgcc gcggcgagat tttccagcgc
    19261 gcgcaccgcc atcgcgaatg accccgctcc gcggacggcg tcgttgacct cggcggtggc
    19321 accgtccagt gagatctgga catccacgta atcactggcc gccagccgcg cggccacctc
    19381 cggggtgatg cggaccccgt tggtggagaa cttcacgccg acatggtggg cggtggcgta
    19441 atcgaccagc tcccaaaagt ccgggcgcac agtcggttcc ccgccgccga tgttgacgta
    19501 gaacacctgc atgcgttcca gctcgtcgat gacgtcctga cattgacggg tggacagctc
    19561 gcgtggatcg cgtttgcccg acgacgacag gcagtgcaca catgccaggt tgcaggcgta
    19621 ggtcagctcc caggtcaggc agatgggtgc atccagtccg cgctcgaact gttcgatgag
    19681 ccggggcacg gttgtggctg ggacggtcac tggtcctccc gtgctaccag catgttggaa
    19741 gcggccagca cacccaacgc gtgcagatac ggcgcctgcc cggcgtcgcc gatccccgcg
    19801 gcgcggcagg cggaccgggc gtcggcgtgg tcggccagcg accgcaccac ggtgaggatg
    19861 gtggggttct tcagaaacga cagcttgcgg gtgccgaagt gatagagcag cgcaccaaac
    19921 gactccggcc gaatcgccac ctgcgggtgc aaccgccagc cgcgatcagg atcgaagacg
    19981 cccggcgacg atgcgggccg aaccggggcc cgctgaggag ccgggcaatc gagccctgca
    20041 ctcggcgacg gtgcgggcgg tactgcgacc gagtctgcgc gcgcgggcgc ggccacggtc
    20101 agtagacccc gcacatgccg tcgatggaca cctcttcgac gagggtctcg gtgacgagct
    20161 cggagtcggt gtcgatctcg tggtccatgg gcataacctc tctcgtgaag gctcgacgac
    20221 gattgatctg gatcacaatc gtcgtcataa tatggcatcg agtgccgaaa cgaaaggttc
    20281 cgatgatgcc ggagtcgcgg gtgggcaggc gccgctcgac gacgccgcac cacatcaccg
    20341 acgttggcat cgagttgttc agcgcgcgcg gttttggcga ggtgagtgtc gacgacgtcg
    20401 cgcaggccgc cggcatctcc cgccgaacgc tcttccgcta ctacgcgtcc aagaacgcca
    20461 tcccctgggg cgacttcgac acccacctcg agcacctgcg ggacctgctc gacaacatcg
    20521 atcgagcggt gcccctgggc gaggcgctgc gcgcggcgct gttggcgttc aacacttttg
    20581 acgaatccga gaccgtccgc caccgccagc gcatgcgggt gatcctgcag acccccgaac
    20641 tgcaggcgta ttcgatgacc atgtacgccg gctggcgcga agtgatcgcc ggcttcgtgg
    20701 cgcgccgcct ggacggcaag acgaccgatc tgctgcccca gaccgtggcg tggacgatgc
    20761 tcggggtggc gctcgccgcc tacgagcatt ggctgggcga cgaatccgtc ccgttgcccg
    20821 tcgcgctcgg cgatgcgttc gacgtcgtgg gcgccgggct ggagaggctg aaccagtgag
    20881 cgccaggccc gagcacctgc tgcacacatt gcgggcgcag ggacagttct gctccgcgtc
    20941 cggctctccg atgtacggcg agctgttcga gctggtggcc ggcgacgtcg aaaccggcgg
    21001 cgtcttcgcg accattttgt ccggccacga ggacgatcca tcgcgccacg cggtgccgct
    21061 tcggctgctc ggcggcctgc accggttggt gctcgacggc cgggtaccgc agttgcgccg
    21121 ctggtatccc agcaccggcg gtagctggga cgccgacgcc gcgtggcccg aaatcctgcg
    21181 caccggcgcc gcgcaccgca actcgctgcg cgcggcgctc gaacacccac cccagaccaa
    21241 cgaggtgggc cgttccgccg cgctgatcgg cggtttgctg cgcgtcaatc gcgaattcga
    21301 tttgccggta aggcttttcg agatcggatc gagcgccggg ttgaacctgc gggccgacca
    21361 ctaccactac cggtaccccg gcggccaatg gggatcggtg gaatcgccgg tgaccatcga
    21421 cgacgcgtgg cacggcgcgc tgccgccgca gggtgcggtg cgaatcgtcg agcgaaacgg
    21481 ctacgacatc gcgcccatcg acgtcaccgg cgccgacggc gaaatgaccg tgttgagcta
    21541 cgtctggccc gaccaggaca cccgactgcg ccggctgcgc ggcgccgtcg ccgtcgcccg
    21601 gaacgtcccg gcgcggctgc atcgcatcac tgccgccgcg gcggtggccg ggctgaggcc
    21661 ggccgagggc acgttgaccg tgctgtggca ttcgatcacc tggcagtatc tgtccgacga
    21721 cgagcgcgcc gccgtccgag ccgggatcga cgcggtgggc gcacaagccg atgcgcgctc
    21781 gccgttcgcc cacctcacac tggagcccgc gcgggacgga cccggcgcgc cgatcaggtt
    21841 tttggtgcga gcccgtagct ggccgggtgg cgagctcgcg atcctcggcg aatgccatcc
    21901 gcacgggccg ccggtgcact ggcagtagat tttctaggtc tcggggttgg cgatccggat
    21961 gggcgctccg tccagccagg ccaccacggc ttcgatggtg tcggagtaga aggcactcag
    22021 catttcccgg gtgacgtacc ccaggtgggg ggacaacgtc acattcggaa gaagtcgcaa
    22081 cggatggtcg ggcggaggcg gttcgacgtc atagacatcg atcccggctc cggcgatgcg
    22141 cccggtcttc agcgcggcaa tcagcgctgc ctcatcgacg atcgggccac gggaagtgtt
    22201 gatcaggtag gcgtgcggct tcatcagcgc cagctcggtc tggccgacca gacctcgcgt
    22261 gcgctccgag agcaccaggt ggatggagac cacgtcggat tcttcgaaca ccgtcgcctt
    22321 gtcgacccgg cgagcgccga ccgaaaccgc ggtctcctcg gtgaggttgt ggctccacgc
    22381 aatgatttgc atgccaaaca ctttcgcgta ttcggccatg cgtttgccga tcctgcccag
    22441 accgagcagc cccagcgtct tgccggacag cgtcataccg gtggtgacct gccagccgcc
    22501 gtcgcgcatc cggcgatgct cttcggccag attgcgcacc gttgcgatca gcagccccca
    22561 ggcgagttcg ggagtggcat cacgaaccga gctgaagcgc ggattggcga aatccgagtg
    22621 ggcgaccagg acaccgcatt cgctggccgc ggccatgtcc aagttcggca ggctcctgcc
    22681 gacgatggtg atcagtctca ggttcggcaa ccgctcgatg agtgtgcgcg gaagcgccat
    22741 ccgctcgcgc agggtgcaga tcacatcgaa gggccgcagc gcctccgcgg cctcgtcctc
    22801 ggaaaggtgc cggtcgaaga cggttacttc ggatctcgtc tgcaccggcg accagtcggc
    22861 caagtcgagg gccaggccgg cgtaatcgtc gagtatcgcc acccggggca cgcggtcagc
    22921 ccaatacttc ggcgatgggg gtagcggcgg cgatcttggc gcggaccttc ataaccttgc
    22981 cgggcatccc accgccgacc acgccgacca gcacgccatc gcgctcgtaa taagccagga
    23041 acttgcgtcc gtcgtcctcg acgagatgca cgatgtcgtc ggcttccggc tcccccagac
    23101 actggatctt gacgtcgtac tggtcgctcc agaaatacgg aacaaccgcg ttggtgggca
    23161 cgtcctgccc gagcatcgcg ggcaccacca cgcgggcctg gtcggcgacg ttgctccaat
    23221 gttccacgcg cgcttgatgt cccgtcgcat cacgccagga ggcgacgtcg cccagcgccc
    23281 acacgttcgg ggcgctggtg cgtccggctt cgtcgcagat gacgccgttg tcgacttcga
    23341 taccgctgcc ctcgagccac tcggtcgccg gccgggatcc gatgccgacc acgaccaggt
    23401 ctgcagacag ctccgtaccg tcggtcagca ccacggtgtc gacacactcc tggccgcgga
    23461 cttccgccac acccagaccg aggcggacat cgacgccctc accgcggtgc agccgcgcca
    23521 ccagctcgcc gatctgttcg ccgagcaccg acgcgagcgg cgtcggctgc ggctccacca
    23581 gcacgacgtc caccccgagg ctgcgcaggc ttgccgccac ctcgcagccg atgaaaccgg
    23641 caccgatgac cacggcgtgc cgcgccgcgg acgcgtgttc gcgcaacgcc atgctctcgt
    23701 cgaccgagcg cagcacccga atcccctcga gatcggggaa cgccggaatg cgccgcggca
    23761 ccaacccggt cgcgatgacg agctcgtcgt acccgagtac cgttccgtca tcgagagtga
    23821 tcgtccgctc cgcggtgtcg agaccgacga cggccgaacc cagacgcagg gcgatgtctt
    23881 tctcgtcgta ccactcgcgc ggtttgaggg ccacgtcgtc gacttccttg cgcagcacct
    23941 ccttggacag cggcgggcga tcgtagggca ggtgcacctc gtcgctgacg atggtgatgc
    24001 ggccggtgta ttgcgagcgg cgcagctgct cggccgtccg ggcggcggcc aacccaccgc
    24061 cgacgatcac gattcccttt tcgctgcgct cggcggcttc gctgctggtc acgtggcgtt
    24121 catacacgat ggcttgtcaa tacttcaagg cgcccttgtc gacggggatc tgcgtgccgg
    24181 tcaccgtgcc cgagccgtcg ccggccaacc agacaacgac gtcggatacc tcatcggccg
    24241 tcatgaaccc tttgtactgc aacggcattg gcgggaagct gtgcacataa cggggatgct
    24301 tggcgaatat ctgggccatc gcccccggct cgatcatcgg ggtgtcgacc gagtaggggt
    24361 ggatggaatt gacccggatg tcgtattcgc cgagctcgat cgcgagcgtg ttggtcagtg
    24421 cggtaaggcc gtacttgctg gccgcgtagt ggccgttgcc cggggtggcc ctcaggcccg
    24481 ccgccgagct gacgacgacg atggagcctc cgttgcccgc ctcgatcatc gcgggaatgg
    24541 cggctcgcag ggtgcgccac gtgccggtca ggttgacacc gatcaccgtg tcccactgct
    24601 catcggtgag ctcccaaacc cggccccagc ccagcactcc ggcgttggcc accacaatgt
    24661 cgagccgacc gaattgttcg acggcgtcgg ccaccagatg ccgcagcgcg gcgtcgtcgc
    24721 ggatgtccgc ctcgcgggcc agtaccttgc ggccctcggc ttcaaccaag cggacggtct
    24781 cggcgagatc ctcgggggtg gccggctggt aagtcagggt gtgagacgcc ggtgcgcaga
    24841 tgtcgagcgc gacgatgtcg gcgccttccc gagcaagtcg caccgcgtgc gagcgccctt
    24901 gggcccgggc ggcgccggtg acaaacgcca cccgtccctg cagtgatcca gcatgtccag
    24961 ccactcacgg tccctttcgc tgcgccgtct cggcaactga caggctagca gcgaaactga
    25021 aacgtgttct aatcgcccgg cgacgatctc gtgtgccgaa cgtgcggcca gccgcacgtt
    25081 cggtgccgag cgtgcggcca gccgcactat cgggggatgg tgccccagca gctagccggg
    25141 caattcagcc cagcccggga atcagatctc ggagatgatc tgggcccgtc ggctggcgta
    25201 ctcctgatcg gtcagggcgc cgccggcgcg cagcgtctcc agctcctgga ggcgctgggc
    25261 gatggactgc tgcggtggcg tgacgacggg tgccgggccc gcgggggccg ccggcgcggc
    25321 agccggtgcc tgccgttcgg ccgcccggcg caccacggcc tgaatctgtt ggcgcagcgc
    25381 cggattggac cggatatcga ccatccggtt gagcggaaca ttgttggcct tgaggatctg
    25441 caggatctcc atcagcgggc cggcctgccc gctgagatcg taagacctgt tgtcctcggc
    25501 aatcgtgaac tgcgccggca tcaacccgtt gaccaaagcg cttcgctccc agtcgattcg
    25561 gtattcctgg gtggtcgggt tgaccagaac cacgaccttg cgggcgttga ggttgccgag
    25621 ccgggtgacg ctggcgatga cccggtcttc gctgtcgaac ggcaagaacc cgggcccgga
    25681 gatgtgcagg tgcaccttca ccacctgttg gttattgatc cacgtgcccg tctcggtgag
    25741 cccggtgatc tgcgccagcc ccagcacgcc gttctgctcg agcgccgcgg tcttggccga
    25801 tgacttcgcc ccgtagttgg ccaacgcgac cgcgatcacc acgtcggcca cggtgatcag
    25861 cgcgccgacg tacagcatcc actgcagcac gttgtcgagc ccgagggtga agtagacgac
    25921 caaaaagatc ggaccgacca ggccgccaca cagcaaaacc accagctgtg ccttcaggta
    25981 gcgcgccaac acctgtctct cctcaccttg ctgggaagtt cgtgccagat tagcgccgtg
    26041 gtgaacgcgg tgcgcgcaaa ccgccacggc cggctatcag cggtcattcg ccggcagttt
    26101 ccgccgattg ggccgttccc ggagagtagg caggccccgc cggccgtatc gtcgccgaag
    26161 aattggcggg cgctgccgcc ggcaccgcgg ccagcggcgt gaccggcaag gccgatcccg
    26221 cggccagcgg cgccccaccg atgccgagct cttcggcgcc ttccgcggcc gccgccccca
    26281 cgccgccgga tccgaccagc ccgccgttcg cgccggcaaa acccgcaccg cccaggctct
    26341 cgccggcggc caagctgccg gcaaagccgg ccccgccggg cccgaagaat gcgcccccac
    26401 caccggttgc cgcggcggcc gggctgacgc tgctcgcggc gacgccgacc agtcggcctg
    26461 ccgcgccgtt gccgccggtg ccaaagccgc cggacccgct ggtcccgccg gtcccgctcg
    26521 tcagcgcccc gttggcgccg ctgctcatcg ccggactctg cgccagcgcc tggctgggtg
    26581 tagcgatcgc gctcgacaat tcttgcccga cggcttgcag cggcgatgcg ttcgcggcct
    26641 cggtcgcggc atactgcgcc gcgccgccgc tcatgagctg tacaaactgc tggtggaaca
    26701 acgcggcctg ggcgctgagc gcctggtagg cctgcgcgtg cgcctcaaac agggccgcga
    26761 cggtcgccga cacctcatcg gcgcccgcgg ccagcacccc cgacgtcggg cccagagccg
    26821 ccgcactggc cgagctgatc gtcgaaccga tgtttgccag gtccgtcgcc gcggacgcca
    26881 caaactccgg cgccgcgaac acgaacgaca tccgaacctc ccggcgagta cacgacaaac
    26941 ctaacgattc gcccggtcgt cggacacgga ctcaataagc gcagatcgta tcctggcaaa
    27001 ccgagtcccg ccaagataac gccgaaccgc gacgcacaag gcccggactg ccgaagcggg
    27061 cagtccgggc cttgtactga cttcgcgtcg agcagaccta cttgatgatc ttgacgaccc
    27121 ggccggcgcc gacggtgcgg ccaccttcac ggatcgcgaa ccgcaggccg tcgtccattg
    27181 cgaccggctg gatcagcttc acgttgatgt tggtgttgtc accgggcatc accatctcgg
    27241 tgccctccgg cagcgtcacc acaccggtca cgtctgtggt gcggaagtag aactgcggac
    27301 ggtagttgtt gaagaacggc gtgtgccggc cgccctcgtc cttggacagg atgtagacct
    27361 ggccctcgaa ctcggtgtgc ggcgtggtgg tgccgggctt gaccacgacc tgaccgcgct
    27421 cgacgtcctc acgcttgata ccacgcagca gcaaaccgac gttgtcgccg gcctgtccct
    27481 ggtcgagcag cttgcggaac atctccacgc cggtgacggt ggtcttggtg ctggtcggct
    27541 tgataccgac gatctcgact tcctcgttca cgttgatccg accgcgctcc acacgaccgg
    27601 tcaccacggt gccgcggccg gtgatggtga agacgtcctc gacgggcatc aggaatggct
    27661 tgtcggtctc gcggacgggg tccgggatcg actcgtcgac cgcgtccatc agctgctcga
    27721 cggactcgac ccacttcgcg tcgccctcga gcgccttcag cgccgagacc cgcacgaccg
    27781 gggcttcctc gtcgaactcc tgggcggcca gcagctcgcg gacctccagc tcgacgagct
    27841 ccagcagctc ctcgtcgtcg actgcgtcgg ccttgttcag cgcgaccagg atgtagggca
    27901 ccccgacctg acgggccaac agcacgtgct cacgggtctg cggcatcggg ccgtctgtgg
    27961 cggcgaccac caggatcgcg ccgtccatct gggccgcgcc ggtgatcatg ttcttgatgt
    28021 agtcagcgtg acccggggcg tcgacgtggg cgtagtgccg cttgtcggtc tggtactcca
    28081 cgtgcgcgat gttgatggtg atgccgcgct gacgctcctc aggagcgttg tcgatctggt
    28141 cgaacgcctt cttctcattc aggtccggaa atttgtcgtg caagaccttg gtgatcgccg
    28201 cggtcagtgt ggtcttgccg tggtcaacgt gaccaatggt cccgatgttg acgtgcggct
    28261 tggtccgctg gaacttcgcc ttcgccactg ttgtgtcctc cgacttgttg gtgctttgta
    28321 agcaatgttg atgttttcag tgatgccgcg gtgttaaccg ggccaggtta ctccctggtg
    28381 tcctgccgat cacggcccgg taccgcatcc gtcagcagcc gttggctgct cactggcctg
    28441 tcgccttcgc gatgatctcc ttcgacacgt tcgccggcac ttcggcgtac gagtcgaaca
    28501 ccatggagta gttcgcccgg ccttgagtct tggaccgcag gtcgccgacg tagccgaaca
    28561 tctccgacag cggcacgtgc gcccgaacga cgcgcgcacc cgcccgctcc tccatggcct
    28621 gaatctggcc acggcgggag ttcaggtcgc cgatcacgtc gcccatgtag tcctcgggcg
    28681 tggtgacctc gacagccatg atcggttcca ggatcaccgg ttgcgcttgc tgcgcagcct
    28741 ttttcagcac ctgggaaccg gcgatcttga acgccatctc cgacgagtcg acctcgtgga
    28801 aggcgccgtc gagcagggtg accttcaggt tcaccaatgg gtagccggcc agcacgccgt
    28861 actgcatggc ctcctgcgcg ccggcgtcga ccgacgggat gaactccctg ggcacccgcc
    28921 cgccggtgac ctcgttggcg aactcgtagc tggggccgtc cggagtcatc gccagcggtt
    28981 ccagcttgac gaccaccttc gcgtactgcc ccgagccacc tgtctgcttc ttgtgggtgt
    29041 actcctcctt gtcgacagtg cgccggatcg tctcccgata ggccacctgc ggcttgccga
    29101 cgttggcctc gaccttgaac tcgcggcgca tccggtcgac caggatctcg aggtgcagct
    29161 cgcccattcc ggagatgatt gtctgtccgg tttcct
//
LOCUS       DASSGQ010000076         2729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_157425,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000076 DASSGQ010000000
VERSION     DASSGQ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157425"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            538..1119
                     /locus_tag="VFI55_03505"
     CDS             538..1119
                     /locus_tag="VFI55_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073133.1"
                     /translation="MAGIAATGAVVLAGTAAQLIDYGFLDGRVAALDSATDGGIFGVV
                     GDVSLALATAAAWTVLIRMRPVTAATVALPPILTFLTIDKAFRFHDHIPHWIVFYLPL
                     LAAVFVAAVSIARRLSSRCFRVIAIALAMLAGAFLINRSGDWILREVGGWDNGWMWQV
                     KAVVKHGLEVAGWLLMALGLYVGVEEYVDLRSV"
     gene            complement(1259..2074)
                     /locus_tag="VFI55_03510"
     CDS             complement(1259..2074)
                     /locus_tag="VFI55_03510"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7073134.1"
                     /translation="MNIQIGDVTLHVRDIGDGSPVVLLHGWPDTGELWAHQSRTLAAA
                     GYRTIVPDMRGFGASSKPTDVGAYAAPAQVGDVVGILDALGVDRSHLVGHDWGAAIAW
                     MTAAFAPDRVTSITALSVGHPSSFATAGWQQRQLSWYMLLFQFPGVAEQWLSADGFRN
                     LRQWSSHPQIETVIQRLTEPGALTASLGVYRANLPPESLIAPPRELPPVTAPAMGVWS
                     SHDLALTEENMTNSAKYIAGPWRYERVEGAGHWLQLDAPGEIDALLLDFLDSV"
     gene            complement(2071..>2729)
                     /locus_tag="VFI55_03515"
     CDS             complement(2071..>2729)
                     /locus_tag="VFI55_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7073135.1"
                     /translation="ALRALREPAAWSTAIARYPLAAHWIDAEPITAVDVMAGMEDRLR
                     RYVVAGDPVASGVVAVGDAWACTNPSLGRGASFAIIHARVVRDVLRDVGVDDHDKLVR
                     RFDEATTQTVEPLYRATNTFDTHRLAEIDGDVAGLPYRPEDPRWVGSKALFAAALRDP
                     DALRALLSITSFLATPDEVFATPGLLDRVLALGVTAPQYPLPGPSRAELLGAITGGIR
                     "
BASE COUNT          442 a          843 c          976 g          468 t
ORIGIN      
        1 gtcgaggggt tcggtgaggc gctttcgggg ttgcggcgcc ggcattgtga gatctccacc
       61 gccggaatcg ttcccctgtc gaagggctga tcacccgtca gcgaaggcat ttgtcaggtg
      121 aaaggttgcc gggatgcgtc cgaagatcag tatcaggccg gacgcaattt cggcttggct
      181 gtggccgccc aagcggtgtg gtacctggcg tcccattgca tttgggcatc aatgatgcgg
      241 aaccataatg caacggcgtc cgcgggggca tccggtgaga tgggcaagaa tcccgcgggt
      301 tccggacagg atacgtccgg cagagatcaa gatatggcgt acggagaagc ggcacggtaa
      361 acatctacca agtgaacctt tttcaacgtg gccagggcgg cagtggccgg cgcaggccaa
      421 cggcccggac cccttcggcc cgacgccagg ttgaaaaagg ttcagtgcat gccctagtgc
      481 ccgggaggga tcttgctcct cgccagaacg caccgcgcaa ggacaaagca cctcatcgtg
      541 gctggaatcg cagcgacggg tgcggtcgtt ctcgctggaa ccgccgcgca actgatcgac
      601 tacgggttcc tcgacggacg cgtggcggca ctggactcgg ctaccgacgg gggaatattc
      661 ggagtggtcg gcgatgtctc cctcgcattg gcaacggctg cggcatggac agtgctgatc
      721 cgaatgcggc ctgtaacagc agcgacggtg gcgctgccgc cgattctgac tttccttacg
      781 atcgacaagg cattccgttt ccacgatcat attccgcact ggatcgtgtt ctacctgccg
      841 ctgctggccg cggtctttgt cgccgccgtg tccatagccc ggagactgtc gtcgcgttgc
      901 tttcgggtca ttgcgatcgc gctcgccatg ctggccggag cattcctgat caatcgatca
      961 ggggactgga ttctgcgcga ggtcggcggc tgggacaacg gctggatgtg gcaggtcaaa
     1021 gctgttgtga agcacgggtt ggaagttgcc gggtggctgc tgatggctct cggactgtat
     1081 gtgggagttg aggagtacgt ggatctccgc tcggtgtaac ggccggatat caattcggcg
     1141 atttcgtctc gctgctccgc cgccaatgct cgaagaccag gtggtggtcg attctcctgc
     1201 gggtttcctc ccggcgcagg ctcagccctg cccgggccag gagaatgcga tgcgacacct
     1261 acacggaatc caggaagtcg agcagaaggg cgtcgatttc gccgggcgca tcgagctgaa
     1321 gccaatggcc ggcgccctcg acccgttcgt agcgccacgg cccggctatg tacttcgccg
     1381 agttggtcat gttttcctcg gtcagcgcga ggtcgtggct gctccacacg cccatggcag
     1441 gtgccgtcac cggcggaagc tcccgcggcg gggcgatcag cgactcgggc ggcaggttcg
     1501 cccggtagac gcctaggctc gcggtcaacg cgcccggctc ggtcagccgt tggatgaccg
     1561 tttcgatctg cgggtgtgac gaccactgac gcaagttgcg gaatccatcg gcggacagcc
     1621 actgctcggc gacgccgggg aactggaaca gcagcatgta ccaggacagc tgccgctgct
     1681 gccagccggc cgtcgcgaac gacgacggat gcccgaccga gagcgcggtg atcgaggtga
     1741 cccggtccgg cgcgaatgcc gcggtcatcc acgcgatcgc ggcgccccag tcgtgtccga
     1801 cgaggtggga acggtcgacg ccgagcgcgt cgaggatgcc cacgacgtcg ccgacctgcg
     1861 ccggcgcggc gtacgccccg acatcggtgg gtttgctgga cgcgccgaag ccgcgcatgt
     1921 ccggcacgat cgtccggtag ccggcggcgg ccagggtccg gctctggtgc gcccacagtt
     1981 cgcctgtgtc gggccagccg tgcaacagca cgactggcga gccatcgccg atgtcgcgta
     2041 cgtgcagggt gacgtcgccg atctggatgt tcaacggata cctccggtga tggcgccgag
     2101 tagttcggcg cgggacggtc cgggtagcgg gtactgcggc gcggtgacgc cgagtgccag
     2161 gacgcggtcg agcaggcccg gcgttgcgaa gacctcgtcg ggggtggcga ggaacgaggt
     2221 gatcgagagc agggcccgca gcgcgtcggg gtcgcggagg gcggccgcga acagggcctt
     2281 ggagccgacc cagcgcgggt cctccggtcg gtacggcagg ccggcgacgt cgccgtcgat
     2341 ctcggccagc cggtgcgtgt cgaaggtgtt ggtggcgcgg taaagcggtt ccacggtctg
     2401 cgtggttgcc tcgtcgaacc ggcggaccag cttgtcgtgg tcgtcgaccc cgacgtcgcg
     2461 gagcacgtcc cttacgacgc gggcgtggat gatcgcgaag gaggcgccgc ggccaagaga
     2521 cgggttcgtg cacgcccacg cgtcgccaac cgcgaccacg ccgctggcga ccggatcacc
     2581 ggcgacgacg taccggcgca accggtcctc catgccggcc atgacgtcga cggcggtgat
     2641 cggctcggcg tcgatccagt gcgcggcgag cgggtagcgc gcgatcgcgg tggaccaggc
     2701 cgccggctcg cgcagtgccc gcagcgccc
//
LOCUS       DASSGQ010000077         6437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_157634,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000077 DASSGQ010000000
VERSION     DASSGQ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157634"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3630)
                     /locus_tag="VFI55_03520"
     CDS             complement(<1..3630)
                     /locus_tag="VFI55_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HET7073136.1"
                     /translation="MMRTAFSRISGMSALQRATLTEEFSKISRLAVAEPVAVVGIGCR
                     FPGDVTGPDSFWDLLVEGRNAISRIPADRWDADAFYHPDPLMPGRMTTKWGGFLPDIA
                     GFDAEFFGITPREAAAMDPQQRMLLEVSWEALENAGIPQDSLSGTRTGVMMGVYFNEY
                     QSMLAATPERVNAYTGTGNAHSVTVGRISYLLGLRGPAVAVDTACSSSLVALHLACQS
                     LRLRETDLALAGGVSLTLRPETQIAISAWGLLSPHGRCATFDAAADGFVRGEGAGVVV
                     LKRLTDAVRDGDRVLAVVRGSAVNQDGRSNGVTAPNTAAQCDVIADALRSGDIAPESV
                     HYVEAHGTGTGLGDPIEFEALAATYGLRKGQSDGTCALGAVKTNLGHLEAAAGIAGFI
                     KAVLAVQRGHIPPNLHFSQWNPAINASTTRFFGPTENSQWPAAEGPRRAAVSSFGLGG
                     TNAHVVIEQGPELEPVSAGRGDTELSTLVVTGKTAQRVAGTAGVLADWMAGPGAGVPL
                     TDVAHTVNHHRSRHAKFGTVVARDRAQAVAGLRALAAGRHAPGVVAPRGGSPGPGTVF
                     VYSGRGSQWPGMGRQLLVDEPAFAHAIAELEPVFVEQAGFSLHEVIARGGELVGIEQI
                     QLGLIGMQLALTALWRSYGVQPDAVIGHSMGEVAAAVVAGALTPAEGLRVTATRSRLM
                     APLSGQGGMALLGLDVTATEALIADHPQVTLAIYNSPNQTVIAGPSGQIDALIEEVRA
                     QNRFASRVNIEVAPHNPAMDALQPQMRSELADLAPRTPTIPIISTTYEDLHTSPSFDA
                     EHWATNMRNPVRFQQAISAAGTDHHTFVEISAHPLLTQAVAATLDAAQPDGAYVSVGT
                     LQRDTDDSITFRTNLNSVRTRPPDTPHPAEPHPRVPSTPWQHTHHWITGPGIDAGALV
                     PPGAHGHNGIAVDAAGGDGVLDGWRYQLQWPVRPLADVETSGVARWLAIADRPLSTEL
                     ARAAGPLSRVETLGPEVLDEAAPDLTGALEGVDNVLYAPPVVTEPLDVESGYRMFHAV
                     RRLVMSMTAADSPAKLFVVTRNAQPVSDADRADPAHAVLWGLGRTLALEHPEIWGGVI
                     DLDESMPAELVARCVLDEAAAADGEDQVVYRRGVRRAPRLQRPTVAVPAPATLDGDTS
                     QLVIGGTGNIGPHLIRQLVQMGARTIVAVSRNPGARLAELAGSLEPAGVRLVQIAADA
                     SDPAAM"
     gene            complement(3632..>6437)
                     /locus_tag="VFI55_03525"
     CDS             complement(3632..>6437)
                     /locus_tag="VFI55_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="KR domain-containing protein"
                     /protein_id="HET7073137.1"
                     /translation="TRPPHTPHPAEPHPPIPSTPWQHATHWMSTRRPANSVGSAPRPG
                     TLLGRHTTVTALSASSPSHLWQAKLAPAAKPYQGRHRFHGVEVVPASVVLHTILCAAA
                     ELGYSGLTGIRFEQPIFADQPRLIQVVADHQSISLASSPVTEQPPVRWTRHVTAELSL
                     APADFAVASNEARHANGQPDPIPDPIPDPIPDIAALLALRGVDGLGFKWSVGSWAHKS
                     TGLTVAIDLPPALPEGSTAPLLDAAILVPALADVSDTRLYVPASIEEVWLGDAVTAPR
                     GLVTLNRTARDGDGITVDVAVAAAGAPWASLRSLRYRALDFGDTFGFGGPAESHPDVL
                     EDARTFVHTIDWQPRADLDAAQAHWVSGAGPVAVIGDDSALLRLRLEEAGYPPAPVPD
                     GVDRTSGARYVLYVADSQPATAAETTETHVDFAVRTSAEIKDLVRTLAQREADMPAAL
                     FIVTRGVHESVTPSALRQSFLWGLAGVIAAEHPELWGGLVDLPVDADLDDAGPALANL
                     LHTPSKSILVVRDGAVLAPTLAPVRGKPVRKALRCRPDAAYLITGGMGALGLLMAGWL
                     ADRGARRLVLTGRTPLPPRRDWDRDILQNTQAEARRKIDAIRALEMRGVTVEAVAVDI
                     GRYDDVQALLAKRDRDGAAPIRGIIHAAGVTNDQLVTNMTDDPVRQVMWPKIGGGQVL
                     HDAFPPGSVDFFFLTASAAGIFGIPGQGSYAAANSYLDALARARRRQGCHTMSLDWVA
                     WRGLGFAADAQIVNAELQRMGSRDITPAEAFTAWEYADGYDVAQAVVVPVPSPAGADG
                     STAADTYLIPARDWSHMSATEVRSELENGLRTIIAAELRLPEEDLDTDRPFAELGLNS
                     LMAMAIRREAEQFVGIELSAIMLFNHPTVASFAAYLANIVAPQHDSEVDEMAELSASA
                     GSVLDSLFDHIESTSTEAER"
BASE COUNT         1038 a         2157 c         2223 g         1019 t
ORIGIN      
        1 catcgcggcc gggtcgctgg cgtccgcggc gatttggacc agacgcacgc cggcgggctc
       61 gaggctgccg gccagctcgg ccaggcgcgc gcccggattg cgggacaccg cgacgatggt
      121 ccgggcgccc atctgcacca gctgccggat cagatgcggc ccgatgtttc cggtgccgcc
      181 gatcaccagc tgactggtgt cgccgtccag ggttgccggg gcaggcacgg ccacggtcgg
      241 ccgctgaagc cggggcgcac ggcgaacacc gcggcggtag acgacctgat cctcaccgtc
      301 cgcggcggcg gcttcgtcca gcacgcaccg cgcgaccagt tcggccggca tcgattcgtc
      361 gagatcgatg acgccgcccc agatttcggg atgctccagc gccagtgtgc ggcccaggcc
      421 ccacagcacc gcatgcgcgg ggtcggcccg gtcggcgtcc gagaccggct gggcgttgcg
      481 ggttacgaca aacaacttcg ccggcgagtc ggcagcggtc atggacataa ccagccgccg
      541 cacggcgtgg aacatccggt atcccgactc gacgtcaagg ggttcggtca cgacgggcgg
      601 cgcgtagagc acgttgtcga cgccctcgag agcgccggtg aggtctgggg cagcttcgtc
      661 gagcacctcg ggcccgagag tctcaacccg cgacagcggg ccggcggcgc gggccagttc
      721 ggtcgacagc ggccgatccg cgatcgcgag ccaccttgcg acgccggagg tctcgacgtc
      781 ggccagtggc cggaccggcc attgcagctg atagcgccag ccgtccagca cgccgtcgcc
      841 gccggcggcg tctacggcaa ttccgttgtg gccgtgggct cccggcggaa ccagagcacc
      901 cgcatcgatc ccggggccgg tgatccagtg atgagtatgc tgccagggcg tgctcgggac
      961 gcgggggtgt ggttcggccg ggtgcggggt gtctgggggc cgggtgcgca cgctgttgag
     1021 gttggtgcgg aaggtgatgg agtcgtcggt gtcgcgttgc agcgtcccga cactcacgta
     1081 ggcgccgtcg ggctgggcgg cgtccagtgt tgcggcgacg gcctgggtca gcaacgggtg
     1141 ggcgctgatt tcgacgaagg tgtggtgatc ggtgcctgcg gcgctgatgg cctgttggaa
     1201 gcgcaccggg ttgcgcatgt tggtggccca gtgttcggcg tcgaaggacg ggctggtgtg
     1261 caggtcttcg taggtggtgg agatgatcgg gatggtcggg gtgcgcgggg ccagatcggc
     1321 cagctccgaa cgcatctggg gttgcagggc atccatggcc ggattgtgcg gggccacttc
     1381 gatattgacc cggctggcga atcggttctg tgcgcgcacc tcttcgatga gcgcatcgat
     1441 ctgcccggat ggtcccgcga tcacggtctg gttcggtgag ttgtagatcg ccagcgtcac
     1501 ctgcgggtgg tcggcgatca acgcctcggt ggcggtcaca tccagtccca gcagggccat
     1561 gccgccctgt cccgacagcg gcgccatcag ccgagaccgg gtggcggtca cccgcaaccc
     1621 ctcggcgggg gtgagcgctc cagccaccac ggcggcggcc acctcaccca tcgagtggcc
     1681 gatcacggca tccggctgca cgccgtagga gcgccacagc gccgtcagcg ccagctgcat
     1741 gccgatgagc cccagctgga tctgctcgat gccgaccagt tcaccgccgc gggcgatcac
     1801 ctcgtgcagc gaaaatccgg cctgctcaac gaacaccggc tcgagttcgg cgatggcgtg
     1861 ggcgaaggcg ggctcgtcga ccagcagctg gcggcccatg ccgggccact gggatccgcg
     1921 cccggaatag acgaaaaccg tgccgggacc gggcgagccc ccacggggag cgacgactcc
     1981 cggggcgtgc cggccggcgg ccaatgcgcg caaccccgct accgcctggg cccgatcccg
     2041 cgcgaccacg gtgccgaact tggcgtgccg ggagcggtgg tggttgacgg tatgggccac
     2101 gtcggtcaaa gggaccccgg cgccggggcc tgccatccaa tcggccagca cgcccgccgt
     2161 ccccgccacc cgctgcgccg tcttacccgt caccaccagc gtcgacaact cggtatcgcc
     2221 gcggcctgcg gacacgggtt ccagctcggg accctgctcg atgacgacgt gtgcgttggt
     2281 cccgcccaat ccgaacgacg acaccgccgc ccgccgcggc ccctccgcgg caggccactg
     2341 ggagttctcg gtgggaccga aaaatcgcgt cgtcgacgcg ttgatcgccg gattccattg
     2401 cgagaaatgc aaattcggcg gaatatggcc ccgctgcacc gccagcaccg ctttgatgaa
     2461 gccggcgatc ccggccgccg cttccaggtg accgagattg gtcttgacgg cccccagtgc
     2521 acatgtgccg tcgctctggc cttttcgaag gccgtaggtg gccgctagcg cctcgaactc
     2581 gatcggatcc cccaaccccg tgcccgtccc atgggcttcg acatagtgca cactctccgg
     2641 cgcgatgtcg ccggatcgga gcgcatcggc gatcacatcg cactgcgccg ccgtgttcgg
     2701 tgcggtgaca ccgttggaac gaccatcctg gttgaccgcc gacccgcgca ccaccgccag
     2761 cacccggtcg ccgtcgcgca ctgcgtcggt caaccgtttg agcaccacaa ccccggcgcc
     2821 ctctccccgg acgaacccgt cggcggccgc gtcgaaggtg gcgcagcgac catgcgggga
     2881 cagcaatccc catgcggaga tggcgatttg ggtctcgggg cgcagggtca ggctcactcc
     2941 accggcgagg gccagatcgg tttcgcgcaa ccgcaggctc tggcatgcca ggtgcagcgc
     3001 caccagcgac gacgaacatg cggtgtccac cgcgaccgcc ggaccgcgca atcccaacag
     3061 gtaagaaatg cgtcccaccg tgacgctgtg cgcatttccg gttccggtgt aggcgttcac
     3121 ccgttccggg gtggcggcca gcatggactg atattcgttg aaatagacgc ccatcatcac
     3181 gccggtacgg gtgccgctaa gggaatcctg cggtatgccg gcattttcga gggcttccca
     3241 cgaaacctcg agcagcatcc gttgttgcgg gtccatggcc gcggcctcgc gtggggtgat
     3301 gccgaagaac tccgcgtcga acccggcgat atcgggtagg aagccgcccc acttcgtcgt
     3361 catccgcccc ggcatcaacg ggtcggggtg gtagaacgcg tcggcgtccc accgatcagc
     3421 cggtatccgc gagatcgcgt tgcgaccttc gaccagcaag tcccagaagc tatccggccc
     3481 ggtgacgtcc ccgggaaaac ggcagccgat ccccaccacc gcgacgggtt ctgccaccgc
     3541 gaggcgagag atcttgctga actcctcggt aagggttgcg cgctgcagcg cgctcatacc
     3601 ggagatccgg ctgaaggccg ttctcatcac gctacctttc ggcctcggtc gaggtcgatt
     3661 cgatgtggtc gaacaggctg tccaatacgc ttcctgccga tgcggatagc tcggccatct
     3721 cgtctacttc cgaatcatgc tgcggtgcaa cgatgtttgc cagatatgcg gcgaacgatg
     3781 ccacggttgg atgattgaac agcatgatgg ccgacagctc gattcccacg aactgctcgg
     3841 cttcacgccg aatcgccatc gccatcaggg agttgaggcc gagttcggcg aagggtcggt
     3901 cggtatccag atcctcctcc ggcaatcgaa gctcggcggc gatgattgtc cgtaaaccgt
     3961 tttcgagttc actgcgtacc tcggtggccg acatgtgcga ccagtcccgc gccgggatca
     4021 ggtaggtgtc cgcagcggtg gatccatccg cgcccgcggg cgacggcacc ggcaccacca
     4081 ccgcttgggc gacgtcgtag ccgtcggcgt actcccacgc ggtgaacgcc tccgccggcg
     4141 tgatgtcacg cgaacccatt cgctgcaact ccgcgttgac aatctgtgcg tcggcggcga
     4201 atcccagccc ccgccaggcc acccagtcga ggctcatggt gtggcagccc tgtcggcggc
     4261 gggcgcgtgc caacgcgtcc aggtaggaat tggcggcagc gtaggaaccc tgccccggga
     4321 taccgaatat gcctgcggcc gaggcggtca ggaagaagaa gtccacgctg ccgggcggaa
     4381 acgcgtcgtg cagcacctgg ccgccgccga tcttgggcca catcacctgg cgcaccggat
     4441 cgtcggtcat gttcgtgacc agctgatcgt tcgtgacgcc cgccgcgtgg atgatcccgc
     4501 ggatgggtgc agccccgtcg cgatcgcgct tggccagcag ggcctgcaca tcgtcgtagc
     4561 gcccgatgtc aacggcgacg gcttcgactg tcacaccacg catttccagg gcacggatcg
     4621 cgtcgatctt ccggcgggct tcggcttgag tgttctggag gatgtcgcgg tcccagtccc
     4681 gccgcggcgg caatggcgta cggccggtca gcaccaatcg gcgagcgccg cggtcggcga
     4741 gccacccggc catcagcaga ccgagtgcac ccatgccacc ggtgatgaga tacgccgcat
     4801 cgggtctgca tcgcagcgcc ttgcgcaccg gcttgccgcg caccggtgcc aacgtcgggg
     4861 cgagcacggc accgtcacgg actaccaaga tcgacttact gggagtgtga agcagattcg
     4921 caagtgccgg gccggcgtca tcgaggtccg cgtcgacggg cagatcgacc agcccgcccc
     4981 acagttctgg atgctccgcg gcgatgacgc ccgcaaggcc ccacaagaag ctctggcgca
     5041 gcgcgctcgg ggtcaccgat tcgtgcactc cacgtgtgac gatgaacaac gcggctggca
     5101 tgtccgcctc ccgctgcgcc aaagtccgca ccagatcttt gatctcggcg gaggtccgca
     5161 cggcgaagtc gacatgggtc tcagtggtct cggcggcagt tgccggctgg gagtccgcga
     5221 cgtagagcac gtaacgggcc ccagaagtcc gatccacccc atcgggcacc ggtgcgggcg
     5281 gatagcccgc ctcctcgaga cgcaacctga gcaaggcgct gtcatcgccg atcaccgcga
     5341 cgggccccgc accggacacc caatgggcct gcgcagcatc gagatcggcg cgcggttgcc
     5401 agtcgatcgt gtgcacgaac gtcctggcgt cttccaagac atccggatgg gattcggcgg
     5461 gaccgccgaa cccgaatgtg tcgccaaagt ccagcgcccg gtaccgaagt gagcgtagcg
     5521 atgcccacgg tgcccctgct gcggcgacgg cgacgtcgac ggtgatcccg tcgccgtcgc
     5581 gagcggtgcg gttcagcgtg accaagccac gcggcgcggt gacagcgtcg ccgagccaca
     5641 cctcctcgat gcttgccggc acgtagagtc gcgtgtcgga cacgtccgcc agcgcaggga
     5701 ccagaatcgc ggcgtcgagt agcggcgcgg tcgacccttc gggcagggcc gggggtagat
     5761 cgatggcaac cgtcaggccc gttgacttgt gcgcccacga acccaccgac cacttgaagc
     5821 cgaggccgtc gaccccacgc agcgcaagca gcgccgctat gtcgggaatc gggtccggga
     5881 tcggatcggg gatcggatcg ggctgaccgt tggcgtgccg ggcttcgttg gaagccacgg
     5941 cgaaatcggc cggcgccagt gaaagctccg cggtcacatg ccgcgtccac cgaaccggcg
     6001 gctgctcggt aaccggactg gaggccaggc tgatcgactg gtgatcggcg actacctgga
     6061 tcaggcgcgg ctggtcggcg aaaatgggtt gctcaaaccg gattccggtc aacccggaat
     6121 agcccaattc tgctgcggcg cagaggattg tatgcagcac aacagaagcc gggaccacct
     6181 cgacaccgtg gaatcgatgc cggccctggt agggcttggc ggccggcgcc agcttcgctt
     6241 gccagaggtg ggagggcgaa ctcgccgaga gtgcggtgac cgtcgtgtgt cggccgagca
     6301 gtgtgcccgg acgcggcgcc gagccaacgg agttggccgg gcgccgggtg ctcatccaat
     6361 gcgtggcgtg ctgccacgga gtgctgggaa tgggggggtg cggctcggcg ggatggggag
     6421 tgtgtggtgg gcgggtg
//
LOCUS       DASSGQ010000078        10366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_66125, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000078 DASSGQ010000000
VERSION     DASSGQ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10366
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66125"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..2489)
                     /locus_tag="VFI55_03530"
     CDS             complement(243..2489)
                     /locus_tag="VFI55_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET7073138.1"
                     /translation="MTILNLTLLPLRIALHVADAVLRPSSPAPTPPAELVVVDGMPEG
                     VPPAARRPEPQLPVPSNWPFGEEFPRTCGAGRLAGGALFWTDFLYDDHGATGIPVGDL
                     KIQAPPRGTYVYPDGPAAGNGADIFRVAIGLTDTHTWWRIDWNTLLDASVPIALFTFD
                     TDPARTADEKWPAGAGVRSAGIDMALLVSGRSAAWIDLTTQVTTPVELSVDMAARSFL
                     AQVPRSLVDPTRSWTVRLAAGLANATGDGFADVPAVRGALPGQPNVYNVAFRTNAQEP
                     PHLNFWSDSAQAAALRSGDVSEFAVTVPWAQLAAGETTPEPVITGPSTRWYVSSVELG
                     QGVAAHEILGTKPQFLGRVQPYSICVPSSYAPGRALPLTLLLHSLALGQTQFAAIDPN
                     LLHQVCDVRDSVVVTPLARGPSTWYFDTGELDVWEVWARVAEQFGTDPNRTVVSGYSM
                     GGYATYKFGLSYPQVFSQAVVLAGPPACGVRLLPGVGIPADLDLDSPCAREGDSWKLL
                     ANARWLPYVIAHGLVDELVPFASAAEQVLELDRLGYRHRFTVYPLEDHVVWALQDKFE
                     DPIAHMGTGLRQADPGHITFAWYPQLVRADLGIGPHQVWWVSELTADPAVAARRGAVA
                     EVDARSYARPDPTHTVRHHRGVVLDFDPTPAFYSELDWRVGPPVPALPYLTLRLTGVA
                     SLTVDVARAGLATLASSTIMVATDTAAQITLGGLPAGAEVQLDDHVAGPTVAVPPGRH
                     RITLRVDV"
     gene            complement(2525..3652)
                     /locus_tag="VFI55_03535"
     CDS             complement(2525..3652)
                     /locus_tag="VFI55_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET7073139.1"
                     /translation="MDSHRFEAGVYARRLASAATATADAGLAGLVITPGYDLRYLVGS
                     RAQTFERLTALVLPASGEPTVVLPRLELASLKESAVAELGLAVRDWVDGDDPYQLVSA
                     ALGGGPAATAVTDSMPALHLLPLAGALGVLPTLATEVLRGLRMVKEEPEIDALAKAGA
                     AIDRVHARVPELLCPGRTEADVAADIAEAIVAEGHSEVAFIIVGSGPHGADPHHGYSD
                     RELQVGDIVVVDIGGAYEPGYHSDSTRTYSIGEPTPDVAQQYSVLQRAQRAAFDAVRP
                     GVTAERVDAAARDVLAEAGLAEFFVHRTGHGIGLSVHEEPYIVAGNHVPLAAGMAFSI
                     EPGIYFPGRWGARIEDIVVVTEDGALAVNNRPHELVVVPLS"
     gene            3750..4706
                     /locus_tag="VFI55_03540"
     CDS             3750..4706
                     /locus_tag="VFI55_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HET7073140.1"
                     /translation="MPAPLVLLDGASMWFRSFFGVPSSITAPDGRPVNAVRGFFDSMA
                     VVITQQRPSRLAVCLDLDWRPQFRVDLIPSYKAHRVAEPEPTGQPDIEEVPDELTPQV
                     DMIMELLDAFGIPTAGAPGFEADDVLGTLAARERRDPVVVVSGDRDLLQVVADDPIPV
                     RVLYLGRGLTRATLFGPAEVAEHYGVPVDRAGPAYAELALLRGDPSDGLPGVPGVGEK
                     TAATLLAQYGSLDQILAAVHDPKSKLAKGLRAKLLAATDYIEAAGTVVRVAEDAPVTL
                     STPTDVLPLVAVDPQRTAELATELGVGSSIARLQKALDSLQA"
     gene            complement(4714..5502)
                     /locus_tag="VFI55_03545"
     CDS             complement(4714..5502)
                     /locus_tag="VFI55_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019830717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HET7073141.1"
                     /translation="MSGPQGSDSGHQWQPPGQTGDQSSEPTQVGSPWQQPTEDVTWQS
                     PAYTQPAEYPHYQQPTEHVYPQQYPQQPAYGQPGQYGAQAPQYGQPGQYGQYAQPGQY
                     GQPGQPGQPGQPGQPGQPGQAVHYGQPGQYGQPGQYPQQYQPYEQPGKKRPVALIGGV
                     AGAIAFLLIAAVLVLGFWEPGFFVTTKLDVNKAQAGVQQILTDETNGYGAKNVKDVKC
                     NHGQDPVVKKGGTFDCDVSIDGAAKRVTVTFQDNKGTYEVGRPQ"
     gene            complement(5582..8371)
                     /locus_tag="VFI55_03550"
     CDS             complement(5582..8371)
                     /locus_tag="VFI55_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HET7073142.1"
                     /translation="MTELTELSRFTAELPFKLDDFQQRACAALERGHGVLVCAPTGAG
                     KTVVGEFAVHLALAAGGKCFYTTPLKALSNQKHSDLTARYGRDRIGLLTGDISVNADA
                     PVVVMTTEVLRNMLYADSPALQGLSYVVMDEVHFLADRMRGPVWEEVILHLPDEVRVV
                     SLSATVSNAEEFGGWIKTVRGDTTVVVDEHRPVPLWQHVLVGKRLFDLFDYRNPEADE
                     PADKRRPRVDPDLLRHIAHRREADRMSDWHSPRRQAGRGGAGRGAAGRPRFYRPPARP
                     DVIAILDSQGLLPAITFVFSRAGCDAAVGQCLRSPLRLTTDEERAQIAEVIDHRCGDL
                     ADSDLAVLGYYEWREGLLRGLAAHHAGMLPVFRHTVEELFTAGLIKAVFATETLALGI
                     NMPARTVVLERLVKFNGEQHVPLTPGEYTQLTGRAGRRGIDVEGHAVVLWNPAEDTTE
                     PSAVAGLASTRTFPLRSSFAPSYNMTINLVHRMGPEQAHQLLEQSFAQYQADRSVVGL
                     VRGIERGKQMLGEIADELGGPEAPILDYARLKARISEAERAQSRASRLHRRQAASDAL
                     AALRRGDIITITHGRRGGLAVVLESARDSTDPRPLVLTEHRWAGRISSADYTGASAPV
                     GSMSLPKRVEHRQPRVRRDLASALRSAAEALTVPKGRRGGDGNGSYEDFHDPELASLR
                     AQLRRHPAHNGANLDPQIREAERYLRIERDNAQLEKKVAAATNSLAKTFDRIVGLLTE
                     RGFIQGPASDPHVTDDGRLLARIYSESDLLVAECLRTGAWAGLKPAELAAVVSAVLFE
                     SRGGDGPSATLAAEVPTQRLRQALQQTSRLSTALRADEHTHRITPSREPDDGFVNVIY
                     RWAQAGDLAAALAAADVTGIGSPLSAGDFVRWCRQALDLLDQVRNAAPDPELRAAARR
                     AINDVRRGVVAVDAG"
     gene            complement(8423..9379)
                     /gene="tatC"
                     /locus_tag="VFI55_03555"
     CDS             complement(8423..9379)
                     /gene="tatC"
                     /locus_tag="VFI55_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET7073143.1"
                     /translation="MKRLKVSARTAGLLKRLNPRNRRSSTNPDATMSLVDHLTELRTR
                     LLISLAAILITTIFGFVWYSHSIFGLESLGEWLRHPYCSLPQSARAAISADGQCRLLA
                     TAPFDQFMLRLKVGMTAGVVLACPVWFYELWAFITPGLYQKERRFAIAFVIPAAALFV
                     VGAVLAYFVLSKALSFLLTVGSDVQVTALSGDRYFGFLINLLVVFGVSFEFPLLIVML
                     NQTGVLTYQRLKSWRRGLIFGMFVFAAVFTPGSDPFSMTALGVALSVLQEFAIQLARL
                     HDKRKAKREAQAVIPDDQASVIERPSPIPEPSFIAGQHDDVT"
     gene            complement(9420..9707)
                     /gene="tatA"
                     /locus_tag="VFI55_03560"
     CDS             complement(9420..9707)
                     /gene="tatA"
                     /locus_tag="VFI55_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HET7073144.1"
                     /translation="MGSLSPWHWAILAVVIILLFGAKKLPDAARSLGKSMRIFKSELR
                     EMQSEGKAETPSLQTPPVTPAASAPIPQPVQSQRVDPAAPSEQGHTEARPA"
     gene            complement(9816..>10366)
                     /locus_tag="VFI55_03565"
     CDS             complement(9816..>10366)
                     /locus_tag="VFI55_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HET7073145.1"
                     /translation="PVERRAAAAVRAAVRDKKALALDYYAASHDSLTSRIVDPIRVLL
                     IGGHSYLEAWSREAEGVRLFRFDRIVDATELGEPAAPPAPALQAPPDTSLFDGDPSLP
                     SARLRVAPSASWMFEYYPMRDVRELPDGSCEAVMTYASEDWMTRLVLGFGAAVRVLEP
                     ESLARRVRDAATAALQSYQVLN"
BASE COUNT         1675 a         3587 c         3512 g         1592 t
ORIGIN      
        1 atcccaggcc gaacacctcc cccgccgcgt ccaagccagt gtgggccgca aaacccgacg
       61 tcaacacagc caggctggca ttcaagccat cgccggcggc caccacggcg ctgcccgcag
      121 caaacagcgc ctcaggatca actaccagcg gccccacgcg ccgaattctc gcccaggccg
      181 acgcccacca ctattcaccc aggtcagacg gccaacccag ccacaactag ccgcgccaca
      241 actcacacat caacgcgcag cgtaatgcga tgacgtcccg gcggcaccgc cacggtcggc
      301 ccggccacgt gatcgtcgag ctgcacctca gccccggcgg gcagcccgcc gagcgtgatc
      361 tgtgcggcgg tgtcggtggc caccatgatc gtcgacgatg ccaaggtggc cagcccggca
      421 cgcgcgacgt cgaccgtaag gctggcgact cccgtgagac gcagcgtcag atagggcagc
      481 gcggggacgg gcggccccac ccgccagtcc agctcgctgt agaacgcggg tgtcgggtcg
      541 aagtcaagga cgaccccgcg atggtgccgg acggtgtgcg tcgggtccgg tcgggcgtac
      601 gaccgcgcgt cgacttcggc gaccgcccca cgccgcgccg cgactgccgg atcggcggtg
      661 agctccgaca cccaccacac ctggtggggc ccgatcccca ggtccgcacg caccagttgg
      721 gggtaccacg cgaacgtgat gtgtcccgga tcggcttggc gcagaccggt tcccatgtgc
      781 gcgatcgggt cctcgaactt gtcctgaagc gcccagacga cgtgatcctc cagcgggtaa
      841 accgtgaacc ggtgccggta gccaagccgg tcgagttcga gtacctgctc ggcggcggag
      901 gcgaacggca ccagttcgtc gaccagcccg tgggcgatga catacggcag ccagcgtgcg
      961 ttcgccagca gcttccagct gtcgccctcg cgggcacacg gcgagtccag gtcaaggtcg
     1021 gccggaatgc cgacgccggg gagcagccgc acaccgcacg ccggcggacc ggccagcacc
     1081 accgcctgtg aaaacacctg cgggtagctc aacccgaact tgtaggtcgc atacccgccc
     1141 atcgaataac ccgagacgac agtgcggttg gggtcggtgc cgaactgttc ggccacccgc
     1201 gcccacactt cccagacgtc gagttcgccc gtgtcgaagt accaggtcga cgggcctcgc
     1261 gccagcggcg tgaccaccac cgaatcgcgc acgtcgcaca cctggtgcaa gaggttcggg
     1321 tcgatcgcgg cgaattgagt ttgaccgagc gcgagcgagt gcagcagcag ggtcagtggc
     1381 agcgcgcgac ccggggcgta gctggacggc acgcatatcg aatacggctg aacccgaccc
     1441 agaaactgcg gcttggtgcc caaaatttcg tgggccgcca caccctgccc aagctcgact
     1501 gaggacacgt accaacgggt cgacgggccg gtgatgacgg gttcgggagt ggtttcgccg
     1561 gccgcgagct gagcccacgg caccgtcacc gcaaactcgg acacgtcgcc gcttctcaga
     1621 gcggcggcct gggccgaatc ggaccagaag ttcagatgcg gcggctcctg agcgttggtg
     1681 cgaaaagcga cgttgtagac gttgggttgc cccggcagcg ctccgcgcac ggcgggcacg
     1741 tcggcgaatc catcccccgt cgcgttggcc aaccccgcgg ccagccgcac cgtccagctt
     1801 cgggtcggat cgaccaagga gcgcggcact tgcgccagaa atgaccgtgc cgccatatcg
     1861 acgctgagct cgacgggcgt cgtgacctga gtggtcaggt cgatccacgc cgcgctgcgc
     1921 cccgagacca gcagggccat gtcgatgccc gccgagcgca cgcccgcccc ggccggccac
     1981 ttttcgtcgg cggtgcgagc gggatcggta tcgaaggtga acagcgcgat gggcaccgag
     2041 gcgtccaaca gtgtgttcca gtcgatccgc caccaggtgt gggtgtcggt gagcccgatg
     2101 gcgacgcgga agatgtcggc gccgttgccg gccgcgggcc cgtcggggta gacgtaggtg
     2161 ccgcgtggcg gtgcctggat tttcagatcc ccgaccggga tccctgtcgc gccgtggtcg
     2221 tcgtagagga aatcggtcca gaacagggca cccccggcca gccggccggc gccgcaggtc
     2281 cgcgggaact cttcgccgaa cggccagttc gacggcacgg gtagttgggg ctcgggtcgt
     2341 cgggccgccg gtggcacacc ctcgggcatg ccgtcgacca cgacgagttc ggccgggggc
     2401 gtcggtgccg gtgagctggg gcgcaacact gcatcggcga cgtgaagtgc gatgcgaagc
     2461 ggaagcagcg tgaggttcag gattgtcacc acgcccaacc tacgcgggca gggtgtcgcg
     2521 ttcgttaaga caggggcacc acgacgagtt cgtgcggccg gttgttgacc gccagggcgc
     2581 cgtcttcggt caccacgacg atgtcttcga tccgggcacc ccaccggcca ggaaaatata
     2641 tgcccggctc gatggaaaag gccatgcccg cggcgagcgg cacgtgattg ccggcgacga
     2701 tgtacggctc ctcgtgaacc gacagcccga taccgtgccc ggtgcggtgc acgaagaact
     2761 ccgcgagccc ggcctcggcc agcacgtcgc gggcagcggc gtcgactcgc tccgcggtca
     2821 cgccggggcg gaccgcgtcg aaggcggcgc gttgggctcg ctgcagcacc gaatactgct
     2881 gtgccacatc gggcgtgggc tcaccgatgc tgtaggtgcg ggtcgagtcg gagtggtagc
     2941 cgggctcgta ggcaccgccg atgtcgacga ccacgatgtc acccacctgc agttcgcgat
     3001 ccgaataacc gtggtgcggg tcggcgccat gcggaccgga gcccacgatg atgaacgcca
     3061 cctccgaatg cccttcggcg acaatcgctt cggcgatgtc tgcggcgacg tcggcctcgg
     3121 tgcggcccgg gcacagtaac tccggcacgc gggcgtgcac ccggtcgatg gccgcgccgg
     3181 ccttggccag cgcgtcgatc tcgggttcct ccttgaccat ccgcagtccg cgcagcacct
     3241 cggtggccag tgtcggcagc acgccgagcg cgccggccaa cggcaacaag tgcagcgccg
     3301 gcatcgaatc cgtgacggcc gtggccgccg ggccgccgcc caaggcagcg ctcaccaact
     3361 gatacgggtc gtcgccgtcc acccaatccc gcaccgccag gcccagttcc gcgacggctg
     3421 actccttgag cgaggccagc tcgagccgcg gcagcacgac cgtgggttca ccggaggccg
     3481 gcagcaccag cgccgtgagc cgctcgaacg tctgggcgcg cgatccgacg agatagcgca
     3541 ggtcgtagcc cggggtgatg accaggccgg ccaggccggc gtcggcggtc gcggtggccg
     3601 ccgacgccag ccgacgcgca tacactcctg cctcgaatcg gtgagagtcc atgcgagtga
     3661 ggctagtgct caattgcctg gcttctcctc gcccgccctc gcggccgcat cgtcgccagg
     3721 ccaacctggc gcgtgagacc atagccggca tgcccgcgcc attagtgctg ctcgacggcg
     3781 ccagcatgtg gttccgctcg tttttcgggg tgccgtcgtc gatcaccgcc cccgacgggc
     3841 ggcccgtgaa cgccgtgcgc ggattcttcg actcgatggc cgtggtgatc acccagcagc
     3901 ggccgagccg gctcgcggtc tgcctcgacc tggactggcg gccgcagttc cgggtggacc
     3961 tcatcccgtc ctacaaggcg catcgcgtgg ccgaaccgga gccaacaggc cagcccgaca
     4021 ttgaagaagt gcccgacgaa ctcacaccac aggtcgacat gatcatggag ctgctggacg
     4081 cgttcgggat cccgacggcg ggcgcaccgg ggttcgaggc cgacgatgtg ctgggcacgc
     4141 tggcggcgcg ggagcgccgt gacccggtcg tggtggtcag cggtgaccgc gacctgctgc
     4201 aggtggtggc tgacgatccc atcccggttc gggtgctcta cctgggccgc gggttgacga
     4261 gagcgacctt gttcggaccc gccgaggtgg ccgaacacta cggcgtgccg gtggatcgcg
     4321 ccggaccggc ctacgccgaa ctggcgctgt tgcgcggcga tccctccgat ggcctgcccg
     4381 gagtgccggg cgtcggcgag aagacggcgg ccacgctgct ggcgcagtac ggttcgctgg
     4441 atcagatcct ggcggccgtc cacgacccga aatccaagtt ggccaagggt ttgcgcgcga
     4501 aactgcttgc cgcgaccgac tacatcgagg cagccggcac ggtggtgcgc gtggccgagg
     4561 acgcgccggt cacgctgtcg acgccgaccg acgtcctacc gctggtggcc gtcgacccgc
     4621 aacgcaccgc cgagctggcg accgagctgg gcgtcgggtc ctcgatcgcc cgcctgcaga
     4681 aagcgctcga ctcgctgcag gcgtgataat cggctactgc ggccggccga cctcgtaggt
     4741 gcctttgttg tcctggaaag tgaccgtcac gcgcttcgcg gcgccgtcga tgctgacgtc
     4801 gcaatcgaaa gtgccgccct tcttgacgac tggatcttgg ccgtggttgc acttgacgtc
     4861 cttgacgttc ttcgcgccgt agccgttcgt ctcgtcggtt aggatctgct gcactccggc
     4921 ctgcgccttg ttgacgtcca atttggtggt cacgaaaaac cctggctccc agaagccgag
     4981 caccaggacg gcggcgatga gcagaaaggc gatcgctccg gccaccccgc cgatcagcgc
     5041 gaccgggcgc ttcttaccgg gctgctcgta gggctggtac tgctgggggt attggccggg
     5101 ctgaccgtat tgacccggct ggccgtaatg gaccgcttgt ccgggctgtc cgggctgtcc
     5161 gggctgtccg ggctgaccgg gttggccggg ctggccgtac tggcccggtt gagcgtattg
     5221 gccgtactgt ccgggctgtc cgtactgcgg ggcctgcgca ccgtactgcc cgggttgtcc
     5281 atacgcgggc tgctgggggt actgctgcgg gtagacgtgt tcggtcggct gctggtagtg
     5341 cggatactcg gcgggttgcg tgtacgccgg ggactgccac gtcacgtcct cggtcggctg
     5401 ttgccagggc gatcccacct gggtgggttc ggaggactga tcgccggtct gaccgggcgg
     5461 ttgccactgg tggcccgagt ccgatccctg cggtccgctc atcgtttctc ctcagtcccc
     5521 ttgcgtctta cccgaactcg tccggttccc cctaccgatg taacggtagc tcgggcccag
     5581 cctacccggc gtcaactgcg acgacgccgc gccgaacgtc gttgatcgcc cgcctggcgg
     5641 ccgcccgcag ctccggatcc ggtgcggcgt tgcggacctg gtccaacagg tcgagcgcct
     5701 ggcggcacca ccgcacgaag tcccccgccg acaacggcga accgatgccg gtcacgtcgg
     5761 cggcggccaa cgccgcggcc agatctccgg cctgggccca gcggtaaatg acgttgacga
     5821 agccgtcgtc gggttcacgg ctcggggtga tgcgatgggt gtgctcgtcg gcgcgcagcg
     5881 ccgtcgacag cctcgacgtc tgctgcagcg cctgccgtaa ccgctgggtg ggcacctcgg
     5941 cggcgagcgt agcactgggg ccgtcgccgc cccgagactc gaagaggacc gccgagacca
     6001 ccgcggccag ctcggcgggt ttcaggccgg cccacgcgcc ggtacgcagg cattcggcca
     6061 ccaacaggtc gctctcgctg tagatgcggg ccaacagccg gccgtcatcg gtcacgtggg
     6121 gatcgctggc cgggccttgg ataaagccac gttcggtgag cagcccgacg atgcggtcga
     6181 acgtcttggc cagcgagttg gtggcggcgg ccaccttttt ctccaattgt gcgttgtcgc
     6241 gttcgatgcg caggtagcgc tcggcctctc ggatctgagg atccaggttg gcgccgttat
     6301 gcgcggggtg ccggcgcagc tgcgcccgca acgacgccag ctccgggtca tggaagtcct
     6361 cgtacgagcc attcccgtca ccgccccggc ggcccttcgg gaccgtcagc gcctcggcgg
     6421 ctgatcgcag agccgaggcc aggtcgcgcc ggacacgcgg ctgccgatgt tcgacccgct
     6481 tgggcaagga catcgaaccg accggcgccg aggcgcccgt gtagtccgcc gacgaaatcc
     6541 gccccgccca tcgatgttcg gtgagcacca gcgggcgcgg gtcggtgctg tcgcgggccg
     6601 actccaggac caccgcgagc ccgccgcggc ggccgtgggt gatcgtgatg atgtcgcctc
     6661 gacgcagcgc ggccagcgca tcgctggcgg cctgccgccg atgcagccgc gacgcacgcg
     6721 actgcgcgcg ctccgcttcg gaaatccgcg ccttcagccg ggcgtagtcg aggatcggcg
     6781 cctccgggcc gcccagctcg tcggcaatct cgccgagcat ctgtttgccg cgctcgatac
     6841 cacggaccag gccgacgacg gaccggtcgg cctggtactg ggcgaacgac tgctccagca
     6901 gctgatgcgc ctgctccggg cccatccggt ggaccaggtt gatcgtcatg ttgtacgacg
     6961 gggcgaacga actgcgcagc gggaaggtgc gggtggaggc caggccggcc actgcggacg
     7021 gttccgtggt gtcttcggcg ggattccaca gcaccaccgc atgaccctcg acgtcgatgc
     7081 cgcgcctgcc ggcgcggccc gtcagctgcg tgtactcccc cggcgtcagc ggcacgtgct
     7141 gttcgccgtt gaacttcacc agtcgctcga gcaccacggt gcgcgcgggc atgttgatgc
     7201 ccagcgccag cgtttcggtg gcgaacaccg ccttgatcag gccggcggtg aacagctcct
     7261 cgaccgtgtg ccggaacacc ggcaacatcc cggcatggtg ggccgccaaa ccccgcagca
     7321 acccctcccg ccactcgtag tagccgagca ccgccaggtc ggaatcggcg agatcaccac
     7381 aacggtggtc gatcacctcg gcgatctgtg cgcgctcctc gtcggtggtc agccgcagcg
     7441 gcgaccgcaa gcactggccg acagcggcgt cacaaccagc gcgggagaac acgaacgtga
     7501 tcgccggcaa cagcccttgg gaatccagga tggcgatcac atccggccgc gcgggtggcc
     7561 ggtagaagcg cggacggccc gcggctccgc gcccggcacc ccctcgtccc gcctgccggc
     7621 gggggctatg ccagtccgac atccggtccg cctcgcggcg gtgggcgatg tggcgcagca
     7681 ggtccggatc cacccgcggc cggcgtttat ccgcaggctc gtcggcttcg ggattgcggt
     7741 agtcgaacag atcgaacagg cgcttgccca ccaacacgtg ttgccacagc ggcaccgggc
     7801 ggtgctcgtc gaccaccacg gtggtatcgc cgcgcacggt cttgatccag ccgccaaact
     7861 cctcggcgtt gctcaccgtc gccgacaggc tgaccacccg cacctcgtcg ggcagatgca
     7921 ggatcacctc ctcccacacc ggaccccgca tccggtcggc caggaaatgc acctcatcca
     7981 tcaccacata ggaaagccct tgcagcgcag gcgaatcggc gtagagcatg ttgcgcagca
     8041 cttcggtggt catcaccacg accggcgcgt cggcgttcac cgatatgtcg ccggtcagca
     8101 ggccgatgcg gtcgcggccg tagcgcgcgg tcagatcgga gtgcttctgg ttgctcaggg
     8161 ctttgagagg tgtggtgtaa aagcacttgc cgccggcggc cagcgccaga tgcactgcga
     8221 attcgccgac gaccgtcttg cccgcaccgg tcggcgcgca caccagcacg ccgtggccac
     8281 gctccagcgc cgcacacgcc cgctgctgaa agtcgtcgag tttgaacggc aactccgcgg
     8341 tgaaccggga cagctcggtc agttcggtca cgctgccgcc gtgcgacgcc gacatcgccg
     8401 acttctccgg catcgtcgac atctaggtga cgtcgtcatg ttgcccggcg atgaacgacg
     8461 gctccggtat cggcgagggc cgctcgatga ccgatgcttg gtcgtcgggg atcacggctt
     8521 gcgcttcgcg cttggctttc cgcttgtcgt gtaagcgtgc gagctgaatg gcgaactcct
     8581 gcagcaccga cagggcgacc ccgagtgccg tcatcgaaaa cggatcggat ccgggcgtga
     8641 ataccgcggc gaacacgaac atcccgaaga tcagcccgcg ccgccacgac ttgagccgct
     8701 ggtatgtcag cacgccggtc tggttgagca tgacgatcag cagcgggaat tcgaagctga
     8761 ccccgaacac caccagcaaa ttgatcagaa acccgaaata gcggtccccg gacagcgcgg
     8821 tcacctgtac atcgctgcct acggtcagca ggaaactcag cgccttggac agcacaaagt
     8881 acgccagcac ggcgccgacg acgaacagcg cggcggcggg gatcacgaac gcgatcgcga
     8941 agcggcgctc cttctggtag agccccggcg tgatgaacgc ccacaactcg tagaaccaca
     9001 ccggacaggc cagcaccacc ccagccgtca tcccgacctt gagccgcagc atgaattggt
     9061 cgaacggtgc cgtggccagc agccggcact gcccgtcggc gctgatcgcc gcccgcgccg
     9121 actgcggcag cgaacagtac gggtggcgca gccactcgcc gaggctctcc agcccgaaga
     9181 tcgaatgcga ataccagacg aacccgaaaa tcgtggtgat caggatggcg gccagcgaga
     9241 tcagcagccg ggtgcgcagc tcagtcaggt ggtcgaccag cgacatcgtc gcgtccggat
     9301 tggtgctgct gcgcctgttg cgggggttga gccgcttcaa caagccagcg gtgcgcgctg
     9361 aaaccttcag acgtttcacg acgctctgtc agtggcgctc gggcaccgcg gcgacgcggc
     9421 taggccgggc gcgcttcggt gtgaccctgc tcggagggcg ccgccgggtc gacccgctgg
     9481 gactgcacgg gctgcgggat cggcgcggaa gcggcgggag ttaccggggg tgtttgcagg
     9541 gaaggcgtct ccgccttgcc ctcggactgc atttcacgga gctcggactt gaagatgcgc
     9601 atcgacttgc ccaacgagcg cgcggcatcg gggagcttct tggcaccgaa cagcaggatt
     9661 atcacgacag cgaggatcgc ccagtgccac ggactaagac tgcccacttt gattacctcc
     9721 agacgtctac ccgatgctac cgcagcgcag cgacgtgccg gtgcgcccgc gccgcggcgc
     9781 gcgaggtcct cgccggcatt acgcctgagc tttttctagt tcaagacctg gtaggactgt
     9841 agggccgccg tcgccgcatc ccgtacacgt cgcgccagcg actccggttc aagcacccgc
     9901 accgccgccc cgaaacccag caccaggcgc gtcatccaat cctcggaggc ataggtcatg
     9961 acggcctcgc acgatccgtc cggcaactcg cgcacgtcgc gcatcgggta gtactcgaac
    10021 atccaggagg ccgagggggc cacccgcaac ctggccgacg gcagtgacgg gtcgccgtcg
    10081 aacagcgagg tgtccggcgg cgcctgcagc gccggcgcgg gcggggcggc cggctcaccc
    10141 agttcggttg cgtcgacgat ccggtcgaag cggaacagtc ggactccttc cgcttcgcgc
    10201 gaccaggcct ccaagtagct gtggccgccg atcagcagta cccggatagg gtcgacgatc
    10261 cggctggtca ggctgtcatg cgaggcggcg tagtagtcga gggccagcgc ctttttgtca
    10321 cgcaccgcgg cgcgtaccgc cgctgccgcc cgcctttcga cgggcg
//
LOCUS       DASSGQ010000079         5999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1312508,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000079 DASSGQ010000000
VERSION     DASSGQ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312508"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VFI55_03570"
     CDS             <1..1037
                     /locus_tag="VFI55_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7073146.1"
                     /translation="ASSVPHPLAARPNPSAKTTAARVNRALARVPYTRGLHEVADRVW
                     AWTLPDGGYGWSNAGLVAGDGASLLVDTLFDLALTREMLAAMKGVTDGAPIADALITH
                     SNGDHTHGNQLLGASVRIIAAKGTADEIAHGMPPEMLAMTQTANLGPVATPYARDRFG
                     HFDFSGIKVRNADLTFDRELTVEVGGRRVELLNLGPAHTAADSVLHVPDAGVLFGGDL
                     LFIGCTPIVWAGPIANWVAACDAMIALDAPIVVPGHGPVTDPDGIRGVRGYLVHVAEQ
                     AEAAYRKGLSWTEAADTIDLGEYATWLDAERVVVNVYQRYRELDPDTPQLQILALLVM
                     QAEWLAKRSS"
     gene            complement(1119..1988)
                     /locus_tag="VFI55_03575"
     CDS             complement(1119..1988)
                     /locus_tag="VFI55_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7073147.1"
                     /translation="MRFSFAESLTDPTFYIPLAQAAEEAGYSSMTIADSIAYPFESDS
                     KYPYTPDGSREFLDGKEIIETFVLTAALGAVTKTLRFNFFVLKLPVRHPALVAKQAGS
                     LAALIGNRVGLGVGTSPWPEDYELMGIPFAKRGKRMDECIEIVQGLTTGDYFEFHGEF
                     YDIPKTKMSPAPTQPIPMLVGGHADAALRRAARLDGWMHGGGSGPEELDGLIARVKKY
                     RAEEGKTGPFEIHVISGDAYTVDGVKRLEDKGVTDAIVGFRWPYIKGPDTEPLETKIR
                     HLERYAEKVIAKV"
     gene            complement(2213..2986)
                     /locus_tag="VFI55_03580"
     CDS             complement(2213..2986)
                     /locus_tag="VFI55_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015292549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7073148.1"
                     /translation="MPAVSELAESPSSLSVTTRTDQSLVVLTAEGSLDAANGAALRDV
                     IMQAILGEPSAVIVDVSALRVLAESAWSTLIGARWQLRTPPPVPIVLVCADRATREAI
                     TRRQVARFMPVYSTEKGARKALGRLAGRTVRHADAELPANLTSLRESRRLVREWLTGW
                     SQSTFIPVALVVVNVFVENVLEHTGSVPAMRVETDGATATIAVSDGSSTPALRLPSPD
                     KGIDVSGLAIVDALSRAWGSTPTATGKTVWAVIGPENQL"
     gene            complement(3412..4704)
                     /locus_tag="VFI55_03585"
     CDS             complement(3412..4704)
                     /locus_tag="VFI55_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4873 domain-containing protein"
                     /protein_id="HET7073149.1"
                     /translation="MTRPPGPAQRSHHVVIVGAGPSGRSALAALQEAGVTDVVMLDKA
                     PRAATSGHLWPGHEVLGSRFDDDTDTWSLTTTGGEAVRAHVVIAAYRPAHVPFLPDIA
                     GRNDFRGASFHAAQWQPDFDATGQHVAVIGTDATAGHYLPQLTGSAASVTVFAHPPRR
                     IVPELPLATTRAKRWLARPIRRAADRRRSQPTLVGSAISSITTSSIRTADGVDHDVDA
                     IIYGTGFAIPDRISDTTLTGAAGLSVRQAWDDGMEPYLGVAFHGFPNYFFVGGPDVDA
                     QARFVADCVGLMRRTASTRIEVRRSSHCVFNERINLRPAHPGRSAATFKAFDLSSGAR
                     DRDETYDGGATLTVAGTEHPVRVRLAGHLDPIDGHYHWQGTVFSSVSQPLSAAALKRA
                     RTATVTIGARSAPARIVEETPWGTHTVAGVGAPPYPLS"
     gene            complement(4701..5606)
                     /locus_tag="VFI55_03590"
     CDS             complement(4701..5606)
                     /locus_tag="VFI55_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HET7073150.1"
                     /translation="MTTTVKPSGPSREEFSERLLRGSVKKSYEPVVDIDWDTPLDPDK
                     FYLPARLVSLYGTAMWDEMTREQHIELSRQELVNTLSAGIWFENMLNQSLLRTILHED
                     PTSKSTHYKLTELGDETRHMVMFGKAIERIGAKPVRPRRLHRMVINALPLAFQQGSML
                     WVAALIGEEIFDSLQRQMMDDPELQPIIQRLMRIHVTEEARHIQFARDGARKRVAEMP
                     RLNRWFMANINGLGGYFFNYLFSNPIPYARTGLDAKRARRIARTSPHRRETQVAGFAP
                     LAAFLTEIGLMGPIARRGWKRSRFL"
     gene            5733..>5999
                     /locus_tag="VFI55_03595"
     CDS             5733..>5999
                     /locus_tag="VFI55_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET7073151.1"
                     /translation="MGRHIHVIGIGAGDPDYMTVQAIRALNDTQVFFAMDKGEAKSDL
                     VALRREICARFINEPGYRFVELPDPQRAVGVGCAADYRGAVSDWH"
BASE COUNT          958 a         1978 c         2021 g         1042 t
ORIGIN      
        1 gcgccagcag tgtgccgcac ccgctggccg cccgtcccaa cccgagcgcc aaaacgacgg
       61 cggcacgggt caaccgggcg ctagcgcggg tcccttacac ccgagggctc catgaggtcg
      121 ccgaccgggt gtgggcgtgg acgttgcccg acggtggcta cggctggagc aacgccgggc
      181 tggtcgccgg cgacggcgcc tcgctcctgg tcgacacgct gttcgacctg gcgctgaccc
      241 gcgagatgct cgccgcgatg aagggcgtca ccgacggcgc gcccatcgcc gacgcgttga
      301 tcacccattc caacggcgac cacacgcacg gaaaccaact gctcggcgcg tcggtgcgga
      361 tcatcgccgc caagggcacg gccgacgaga tcgcgcatgg catgccgcct gagatgctgg
      421 ccatgacgca gaccgccaac cttggtccgg tggcgacccc gtacgcgcga gaccgcttcg
      481 ggcacttcga cttcagcggc atcaaggtgc gcaacgcgga cctgacgttc gaccgcgagc
      541 tcaccgtcga ggtcggcggc cggcgggtcg aactgctgaa ccttggccct gcgcacaccg
      601 cagcggactc ggtgttgcac gtgcccgacg ccggggtgct gttcggcggg gacctgctgt
      661 tcatcggttg cacgccgatc gtgtgggccg gaccgatcgc gaactgggtc gcggcgtgtg
      721 acgcgatgat cgccctggac gccccgatcg tggtgcccgg ccacggcccg gtcaccgatc
      781 cggatggcat ccgcggggtg cgcgggtatc tggttcatgt cgccgagcag gccgaggccg
      841 cctaccgcaa gggcttgtcg tggaccgagg ccgccgacac catcgacctt ggtgagtacg
      901 cgacctggct ggacgccgag cgggtcgtcg tcaacgtcta tcagcggtac cgcgaactcg
      961 accccgacac cccgcagctg cagatactcg cgctgctggt aatgcaggcc gagtggctgg
     1021 ccaaacggtc atcgtgaccg cgagagtgca accaccaacg catcgcccga gaagggctgt
     1081 cggcagttgc actttcgcgg tcagattttg actgacactt agactttcgc gatcaccttc
     1141 tccgcgtacc tttccaggtg acgaatcttg gtctccagcg gctccgtgtc gggtcccttg
     1201 atgtagggcc accggaaccc gacgatggcg tcggtgacgc ccttgtcctc gagccgcttg
     1261 acgccgtcca cggtgtaggc gtcgcccgag atgacgtgaa tctcgaacgg tccggtcttc
     1321 ccctcctccg cgcggtactt cttgacccgg gcgataagcc cgtcgagttc ctcggggccg
     1381 ctgcccccgc cgtgcatcca gccgtccagg cgcgccgcgc gccgtaacgc ggcgtcggcg
     1441 tgcccgccga ccagcatcgg gatgggctgg gtaggggccg ggctcatctt cgttttggga
     1501 atgtcgtaga actcgccgtg gaactcgaag tagtcgccgg tggtgagccc ctggacgatc
     1561 tcgatgcatt cgtccatccg cttaccccgc ttggcgaatg ggatgcccat cagctcgtag
     1621 tcctcgggcc acgggctggt gcccacgcca agccccaccc ggtttccgat gagcgcggcc
     1681 agcgaacccg cctgtttggc gacgagcgcc ggatgccgga cgggcagttt gaggacgaag
     1741 aagttgaacc gcaacgtttt cgtcaccgca cccagcgccg ccgtcaggac gaaagtctcg
     1801 atgatctcct tgccgtccag gaattcacgg ctgccgtcgg gcgtgtacgg atacttcgag
     1861 tccgactcga agggataagc gatgctgtcg gcaatcgtca tgctgctgta gccggcctcc
     1921 tcggccgcct gcgccagcgg aatgtagaaa gtggggtcgg tcagtgactc cgcaaagctg
     1981 aaccgcacgt catccttacc ttcctcgcag ggactcgtcc tacgacacta gaacgtgttc
     2041 tagtttgacc gacagtcggg ttccccgaca aggcataacc gggatgaggc cgcgtagctg
     2101 cccaggccgg attgccggct ccccggcccc ctgggcctag ccgcatcgtc gtccgcctag
     2161 gccggattgc cggactcctc cccggcccgt tccgggccgc atcgtcgtcc gcctagagtt
     2221 ggttctccgg gccgatgacc gcccagaccg tcttgcccgt cgcggtgggc gtgctgcccc
     2281 aggcgcggga taacgcgtcg acgatcgcaa ggccagacac gtcgatgccc ttgtccggcg
     2341 agggcagcct gagggccggg gtgctgctgc catcggacac cgcgatggtc gccgtggcgc
     2401 catcggtctc gacgcgcatc gccgggacgc ttccggtgtg ttcgagcacg ttttccacga
     2461 acacgttgac cactaccaga gcgaccggaa tgaacgtgga ttgcgaccag ccggtgagcc
     2521 attcccgtac cagccgacgt gactcgcgaa ggctggtcag gttcgcgggc agttcggcat
     2581 cggcatgccg tacggtgcga ccagcgagcc ggccgagcgc ctttctcgcc cccttctcgg
     2641 tcgagtagac aggcatgaag cgggcgacct gccggcgggt gatcgcctcg cgggttgcgc
     2701 gatctgcgca gaccagcacg atcggaaccg gtggtggggt gcgcagctgc cagcgcgctc
     2761 cgatgagggt cgaccaggcc gattccgcga gcactcgcag tgcgctgaca tcgacgatga
     2821 cggcggaggg ttcgccgagt attgcctgca tgatgacgtc gcgcagcgcc gcgccgttgg
     2881 cggcgtccag cgaaccttcg gccgtcagaa cgaccaacga ttggtccgtg cgtgtcgtaa
     2941 cggacaggga gctgggcgac tcagccagct cgctcaccgc gggcacccca aaacactgct
     3001 gtaccagctc gtgtgtcccg acggcaggcc tggcaacctg gtcgaaaacg gttgggacgt
     3061 catattccgg tttcgtagct cgatgttgtc gccgacttcc atttcccgct cctcgaattc
     3121 ccgtccggct gacagccgga gaacccgccc cccttggcgg aaaaatcgtc tagcgccccg
     3181 atgagcggca gctccgcagt ggacgccgaa aatgccgttc tcgcttaatc gtttgctgca
     3241 gcgcaggcac gcagatggat tacccacttc gcctccgcgg ccaacttccg tgtgcgatga
     3301 aatcggccgg gcgagattac ccacacgtaa cagttagcaa acccgcgatg gcccggcaag
     3361 cagcacacag ctcggagtcg ccgcggatat gcgtgccggg cccgccggat gttagctcag
     3421 cgggtacggc ggcgcgccca ctccggcgac cgtgtgcgtt ccccaaggtg tttcctcgac
     3481 gatgcgcgcg ggagcgctgc gggcgccgat cgtgactgtc gcggtgcgtg cccgcttgag
     3541 cgccgcagcc gacagcggct gcgacacgga actgaacacc gtgccttgcc agtggtagtg
     3601 gccgtcgatc gggtccaggt ggccggcgag ccggacgcgc accgggtgtt cagtgccggc
     3661 taccgtcagc gtcgccccgc cgtcgtacgt ctcgtcgcga tcgcgtgcgc cggacgacag
     3721 gtcgaaggcc ttgaacgtcg cggccgaccg gcctgggtgg gcgggtcgca ggttgatgcg
     3781 ctcgttgaac acgcagtggc tgctgcgccg gacttcgatg cgagtgctcg ccgtgcgcct
     3841 catcaggccc acgcaatcgg cgacgaagcg tgcttgcgcg tcaacgtcgg gtccgccgac
     3901 gaagaagtag ttggggaaac cgtgaaacgc gaccccaagg tagggctcca tgccgtcgtc
     3961 ccacgcctgc cggacactca gtcccgcggc gccggtcagc gtcgtatcgg agatccggtc
     4021 ggggatcgcg aatccggtgc cgtagatgat ggcgtcgacg tcatggtcga cgccatcggc
     4081 ggttcggatg ctcgatgtgg tgatgctgct gatcgccgac ccgaccagag tgggctgcga
     4141 tcgtctgcgg tccgcggccc gccggatggg gcgagccagc cagcgtttgg cgcgggtggt
     4201 cgccaacggc agttcgggga caatccggcg tggcggatgc gcgaacacgg tgaccgaggc
     4261 ggctgatccg gtcagctgcg ggaggtaatg accggcggtg gcgtcggtcc cgatgaccgc
     4321 gacgtgctga ccggtggcgt cgaagtcggg ctgccactgc gccgcgtgaa acgacgcccc
     4381 gcggaaatcg ttgcgcccgg cgatgtccgg taggaacggg acgtgcgcgg gtcggtaagc
     4441 cgcgatgacg acgtgagccc ggacagcctc gccaccggtc gtcgtcaggc tccaggtgtc
     4501 ggtgtcgtcg tcgaatctcg aaccgaggac ttcgtggccg ggccacaggt gcccggatgt
     4561 ggccgccctg ggtgctttgt cgagcatcac gacgtcggtg acgcccgctt cctgcaacgc
     4621 cgccagcgcg ctgcgaccgc tcggtcccgc gccgacgatg acgacgtggt ggctacgctg
     4681 cgccgggccg ggcggccgcg tcacagaaac ctgctgcgct tccagccgcg gcgggcgatc
     4741 ggccccatca ggccgatctc ggtcaaaaac gccgcgaggg gagcgaagcc ggccacctgc
     4801 gtctcgcgcc ggtgcgggct ggtgcgcgcg atccgtcgtg cccgcttggc gtccagaccg
     4861 gtgcgcgcgt acggaatcgg attgctgaag aggtagttga agaaataccc gcccaagccg
     4921 ttgatgttgg ccatgaacca tcggttgagc cgcggcattt cggcgacgcg cttgcgtgcc
     4981 ccgtcgcggg cgaactgaat gtggcgggcc tcttcggtga cgtgaattcg catgagccgc
     5041 tggataatcg gctgcagttc cgggtcgtcc atcatctgcc gttgcagcga gtcgaagatc
     5101 tcctcaccga tcagcgcggc cacccacaac atcgagccct gctggaacgc cagcggcagc
     5161 gcgttgatga ccatccggtg caaccgccgc gggcgcaccg gcttggcgcc gatgcgctcg
     5221 atggctttgc cgaacatgac catgtggcgg gtctcgtcgc ccagctcggt cagcttgtaa
     5281 tgggtcgact tgctcgtcgg gtcctcgtgc aggatggtgc gcaacaacga ctggttgagc
     5341 atgttctcga accagatccc ggccgacagg gtgttcacca gttcctggcg ggacagctcg
     5401 atgtgttgct cgcgggtcat ctcgtcccac atcgcggtgc cgtacaacga caccagccgt
     5461 gccgggagat agaacttgtc cgggtccagc ggggtgtccc agtcaatgtc cacgaccggc
     5521 tcgtaggatt ttttgaccga gcccctcagc agacgctccg agaactcttc acgactcggc
     5581 ccactaggtt tcacagtggt ggtcatcgct ggcgtccctt cattgggtgc tgcagacggt
     5641 acccatggta ctgggtactt gcggtcccga ctaggcccct tgtgcgccga ttgggcaatc
     5701 tgaatcgacc agcggcgcgt caggagaatt cggtgggtcg gcatattcac gtgatcggca
     5761 tcggcgcggg ggatcccgac tacatgaccg tccaggcgat ccgggcgctc aacgacacgc
     5821 aggtgttctt cgccatggac aagggagagg cgaaaagcga tctggtagcg ctgcggcggg
     5881 aaatctgcgc gcggttcatc aatgagcccg gttatcgctt cgtcgagctg cccgatcccc
     5941 aacgagccgt cggcgtgggc tgtgcggccg actaccgggg agcggtctcg gactggcac
//
LOCUS       DASSGQ010000080        18344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1251753,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000080 DASSGQ010000000
VERSION     DASSGQ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18344
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251753"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VFI55_03600"
     CDS             complement(<1..340)
                     /locus_tag="VFI55_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET7073152.1"
                     /translation="MLQGIARLAIAAPRRIIAVALLVMVGAGIFGIPVSKSLSAGGLQ
                     DPTSESSQAARLLSEKFGQGDMHLVIAVTSEAGAESLTARAVGTDLVRQLEASHQVAD
                     VNSAWTASPPD"
     gene            complement(445..1362)
                     /locus_tag="VFI55_03605"
     CDS             complement(445..1362)
                     /locus_tag="VFI55_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HET7073153.1"
                     /translation="MTVIERTGGIRAFLVERYLPVYPIYLTLWVVAVESMTAMTGTVT
                     GSAGPWRPTWDTALKVLALIAAGAFLRIVDDQKDLDYDRLHNPTRPLVQGRITHAELR
                     VAMAVTAAVALALAAAVSLPAAAVLAAALGYGLALWWMELHVSWVRDNAIVNLAVVCP
                     AQFLVTGFVMVGQPGVGDASWGRLLAVPVVFTSAFLHLEFARKTCRTGAGDPHSYSQL
                     IGVSASAKTAWAFGMLAVLLELSVTAPWRWAEHWWPVAWVPLAAGILPCVSACIFLGG
                     GADRHPRSWPTVFVIVFYLAIVGQGLVYR"
     gene            complement(1359..2315)
                     /locus_tag="VFI55_03610"
     CDS             complement(1359..2315)
                     /locus_tag="VFI55_03610"
                     /inference="COORDINATES: protein motif:HMM:NF015700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="HET7073154.1"
                     /translation="MNAGNYSRTIARELVHKRSIAEVLITSVETTPATWICHAQLPRR
                     HSFHTDTTGVQSAYHDPLLVLEAFRQACIAGSHACYGVPLDARYTVRYYELSVIDLAA
                     LKCGPHVLDLELDIAVRKEFRRGGEGLQGPVHGLDVAAVLMHEGTKVMELSAAFGWMS
                     LAKWEAFRGDASWDPGPQAAPADPQMVGRTRPENVVIGEPARLDGGAASAAIVVDVSH
                     PTIFDHPLDHLPGGLILEACRQLSLAVLGSSGETITGPAWLRCDFHSFAELDATNTVT
                     VAQSAEDALSFRGEVMQSGQKRASVDLRFTAVDGGHGMSPSA"
     gene            complement(2312..3181)
                     /locus_tag="VFI55_03615"
     CDS             complement(2312..3181)
                     /locus_tag="VFI55_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET7073155.1"
                     /translation="MIRTEVATLAPPETHRRSPSRHASDTYFLETCDALQRLSIDPDE
                     FARRTALLLLKPDAAVTGGMRRAVTWLTEHGYRIVGAQPVGLSRLHLRALWYFNWHSA
                     TPERRRLADRLAGMSASIVLVVTHPDDSVPASVRLTADKGPADPAQRAPGQLRYALAA
                     GTYLLNQVHTPDDPDDVLRELSIYFADPELSEVIAACAAGTDASADAVRLADQIESGV
                     ERRSGDLAVTHDMVWQHLRASGATARPRTGPEWVAAIELADSHHVPVDPWFRIVLESA
                     YLPMHRQTQRGEL"
     gene            complement(3178..4260)
                     /locus_tag="VFI55_03620"
     CDS             complement(3178..4260)
                     /locus_tag="VFI55_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7073156.1"
                     /translation="MNARRVILVTGAAGHVGAEVVARLVVRGHNVVALVHNRFDIVSN
                     NGRPVRPARVLRGDVRRPDFGLDADVVRELAAQVAVIVHCAAVTDFGLPDQRYAELNV
                     AGTANALALARNWDTGLLYVGTAYVCGEFNGTFGEHQLDVGQRFGNGYERSKFQAEQL
                     VRASGQRWAVVRPGIVSGEHRTGHSREHKHIYQVLKLIVEGKLRTLPGNYAATLALSP
                     VGHVADTIATATERFSDNVGRTFHAVGAKCVSLQTLSDILAEYPSFRVADFVPPSTFS
                     VDQLDEIERGYFQKVGTLYASYLTRRLEFDSSNTRDRLGIAAPTTGAGYVRRILDSCL
                     RTGYLGRSGPSIAEVLAALKRERNCA"
     gene            complement(4257..4502)
                     /locus_tag="VFI55_03625"
     CDS             complement(4257..4502)
                     /locus_tag="VFI55_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET7073157.1"
                     /translation="MTAIQNDVLALVTALAPAPAAEPDLHRALIDDLGYTSLRLLELC
                     IAVEHAFALPPLGHDMLAGVSTVGDLVNLVRTQRNCS"
     gene            complement(4499..7879)
                     /locus_tag="VFI55_03630"
     CDS             complement(4499..7879)
                     /locus_tag="VFI55_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7073158.1"
                     /translation="MREPDEADVARILGWLTSPSEDTGVHLAADDGGWEFLSYATLAE
                     QVRRVARLLVEQGVGSGDTVCVLMPTSHLCLATMFAVLAAGATLTPIAPPLFGSHEQY
                     RRHLRGVLESSQASAVVTSSGFVDAVARAAADLPEAPATIVLDGVPAHPPLIELASPA
                     PFVLLQMTSGSTHLPRGARISWRNLTTNLAAIDGACEETGTVDDDATATWLPLYHDMG
                     LIGGIFQAITRQRVLYLMRPDQFVRDPARWLRVAALARNTVSPSFGLAYSARRLTRKD
                     IEGIDLSRIRTMVVGAEPINPDHLRAFTELTAAQGFSPSAFLPSYGLAEHTLLATAHR
                     VGEPHRMVRIDKSALRHGQPVRVLAAATDCLTVESGTDWVMSVGAPIPGHHVRIAHES
                     GLAAAEGILGEIVLSGPSVFGGYRGNPDSATRYIDGELHTGDAGFILDGRLFVLGRMG
                     TSLKVNGRTVFAEDLDVTAAAALGVAPSRLIAAAVNESGRPGVALFVEQRARQLLAGQ
                     IAAARTAVQAQVGEDALVWFIGVPRGGLSRTSSGKPRRAHMWQQWRAGYLVGAALLSM
                     SGPDRDPALLEKVRSLFERTRELAVIPDDATVHFEGSLAEGFGNEGSDIDLLLLVPGA
                     GTEAVMPTVLFVDGHRVEIRVQSHDQVRQRLQRIRRAADTGSLSGITEDLLNRVQRFL
                     RGTVLNIGPRYGELRDVVSYPELTNLLAAWWRRRAGQCLRQAAALALLDHDGEAAHWA
                     REGVTQAMKAFLAERGEGYVEVKWLPLQVERLRARGDRDVAAPLAEYLDLDYPQTAEV
                     GARDFVVRALALSARLGAPTATLDPAKVILRRVPGVTTWPIRSTTHVVRGKADVFVLS
                     PECAESWRHVVFGETLADTRAASTHIALFVRYGLIALGWRGAGTIRPVAAMCDPSRPL
                     TPAPSTRRPVITINGAIADGHIARSPLAAKPFAECAGALMLANMVLENAREDFEGAVK
                     DGQWQVASLCGRRIVTMAVRILTSAWGVTPLPGDPVLVEGLATLIPEHPQLAATARRL
                     VRLSVHDDDEALDARAEIDRFVSDVQAASGGEMFPSSFASPEQWRETLRYGYQWLRMA
                     GYLEAYLELDEARDLLSSGGTQPAARAARSDSESGESA"
     gene            complement(7872..10634)
                     /locus_tag="VFI55_03635"
     CDS             complement(7872..10634)
                     /locus_tag="VFI55_03635"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014982632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HET7073159.1"
                     /translation="MPFLITDQAPGDQLRTLAGGKAHNLHTLTAAGFPVPRWAVLGTD
                     AFHAAVAAAGLQLSDAPLPLSERLREAAHAESVVRDLAIPATILDAIGAALDYVGNTS
                     VAVRSSGRQEDGASHSFAGLFDTILNVTGPVAVEDAVRRCWASAFSERAVRYRHMRGL
                     DLHDIDDFALAVIVQRLVQARSSGVMFTADPVTGSPDRIVINGVLGLGEGLVSGAVDS
                     DSVVVDKRTGKAVDEKVGAKEEMIVTGLGGGVRTVAVPAEQQRHCSIGPDLRDSLIDL
                     GARLEHHFGAPQDIEWAVDESNTWILQSRPITTLTNENPATPAGAEYLGEDVPPGDLR
                     IWDNSNIIESFAGVTSPLTFTTARKLYADVYRAYARSLKVPSAQLDQMESWLPVMLGQ
                     FNGHVYYNLLHWYRMVGIAPGYPLNRRVLEVALGVSEPLDRRTARALRPFGFSNALVK
                     GCSRACTTVTYARRFWSIDALVARFDSSFEEFVDRHGLTDVSALDGPQAYRLFRRIQD
                     DVADIWGPMMVLDAILLTSVGALAALTKTFLPHAPEWFGFAVVNPGTDVVSIEPARAL
                     ADIAAFARDHADLWAFIDAAEPDFAYPLLVDHAGAASAAGAPHWQDLLGKIDEYIDRF
                     GYRCLDELKLEAPDLRQDPSGIFHMLRMASAEETQRAGDSGASVQAYLDAHLRGPRRR
                     LFDALRRKIQRAATHREHLRFCRTQGFGILKSLVAVMGRDLEQRGIVDASADVFLLRL
                     DELFAVYEGSMSRGAAREVIARRAVLHEGYRKLTAPARFATTGVGYSPEELTRAGWRL
                     SGDQTQAPAGTVLNGTPSCPGVVTGRAVVVEKPQDFSSGILVAYRTDPGWVAALPFAS
                     ALLIERASPLTHVAIIARELGVPTVVQIDGLTTAIRTGMTVSVDGASGRITLLEDQPE
                     LGSFDA"
     gene            complement(10988..13783)
                     /locus_tag="VFI55_03640"
     CDS             complement(10988..13783)
                     /locus_tag="VFI55_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET7073160.1"
                     /translation="MRRDQTIAEVSASGGACRDHLRFTRPAVARQMRAFGPEFESCAN
                     PSAEIAFAAREAATGGPLTGRDGELSAIRRALSGAGNQRGAVIVGAAGVGKTRLAREI
                     LAGAEASGARTSWIVGTESARKLPLGAFAASLGQPMIDSLSDVRRVISSFVAQQRQGR
                     VLIGVDDAHLLDGSSAHVVHQLAQMPGTRLLVTVRSGGDQPDAITALWKDHLLNRVDL
                     QPLSSADTRLIIETTLGGAVDSASAQRFWKLTGGNALFTRELLKDQIAAGRMRQVAGV
                     WMWDERVAASDGISGMVGRQLCRLAPDVAMVVDTLSQYEPLEVDMLCDLVRRHDLEVA
                     EQMNLVTVERCGSRLMARLAHPLLGELRRATAGELYLSRIRGKLAQRLAKEDDDDMQA
                     TVRRALLTLESDLAPDRELFLEAARCGMTLLDLDLSDRFATAAADAGAAEATGLRALS
                     LAARGDGDGADAILRQIGMDGADGHHWATVRAANLIWNLGRPTEASTILDGLASARES
                     AAERAERVAVEACADAVAARCETAVEKARAALSSSTVPDFHVMLASSALTMALGALGE
                     IDDVTNHAAEYIDRATTSFQASPMRFWYAAVYAQACRLTGRIDECVGSATQLAGSAPD
                     VPGVVAAYLAFLLGYAELARGQVPTAVKLLHEALAGMENHDVTTGLQAAICFALAEAH
                     AKLGDAEAANDAIAEVRRSVPAEYHFMQTPLAMARGWALAAGGRLREAIAVVQAAGKG
                     AWEPNRPTHELACLQVAAQWGDTSGAKRARELAGLLNLPLANAVARHTKSLRDGSGEG
                     LLAASADYRALGDVATAADVAAQASMAFSRSQQRKRSLYAAALAAQLANECGGLCTPA
                     LRNPADQPLTYRQREIIELAAAGLSNKEIAERLVTSVRTVEGHIYRAYQRLGARSREE
                     LFSIMRSGPARLVCDPA"
     gene            complement(14046..14894)
                     /locus_tag="VFI55_03645"
     CDS             complement(14046..14894)
                     /locus_tag="VFI55_03645"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7073161.1"
                     /translation="MILLHGLLGDGDSWRRQTSHLTSRHPDIRVIAYDQRGHGRSGRG
                     CVLRNTLGQLARDLDAVIEATTPAGDIVLVGHSMGAMTILQYLQHHQSRNTDRITGVG
                     LIATAAADITRYGLGKLLTTPVAGGACALAAHIPASVDAVRITAVRATEPLWRLATKK
                     APTLAAVISAPQLPIVAGFLGSISRFDGTAAYRALSRTPTLIMCGTVDRVTPSNHSAA
                     IAAAVSAAEFVDVPGAGHMLMLDRPDVVNDRLDALLKRTRRELARRPATTAPNCPATV
                     SGPVPA"
     gene            complement(15119..15334)
                     /locus_tag="VFI55_03650"
     CDS             complement(15119..15334)
                     /locus_tag="VFI55_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073162.1"
                     /translation="MSQAPVFITTQEVVFSTAAASSPRPASISRRLIDAIQVVGAALH
                     RPPARRQYPERISYLEHSRMAREMDRL"
     gene            15907..16575
                     /locus_tag="VFI55_03655"
     CDS             15907..16575
                     /locus_tag="VFI55_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7073163.1"
                     /translation="MSGPISVLIAEDCLLVRDSVYRALSTDADVTVVGVAEDYDSTVE
                     LVEKHQPTMLVTDVRMPPTSTDEGIRLARWMRSAHPDTGVIVLSQYADPAYAAGLLDG
                     GTASRGYLLKERVSHFDQLSEAVHQVAAGGTVLDPLVVEALLAQPRSAATLSRLTPRE
                     REVLAELATGLSNRTVARRLVLSQRAVEKHINSIFAKLGLSVDDSVDRRVKAVLMFLD
                     DVSV"
     gene            16669..17043
                     /locus_tag="VFI55_03660"
     CDS             16669..17043
                     /locus_tag="VFI55_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7073164.1"
                     /translation="MRHERVRVWVVDDQASFRLATAATVAATEEFVMAGECETGESAI
                     ELLRDSDAGIVLMDIHMPGMGGIEATRRIRAAHPELMVLLMSTYDLEDLPPAAADCGA
                     ATYLHKEHLSPDLLTRLWRTAH"
     gene            17196..>18344
                     /locus_tag="VFI55_03665"
     CDS             17196..>18344
                     /locus_tag="VFI55_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET7073165.1"
                     /translation="MYFGTGVLLPLWEYQFVLRSRWLLAIVGVCIAQCVVLVVALRLA
                     RRTRYQQSITLVCIGNWASVLLITFVAPVLLPVMVLAALVPVVFAQPYARWQRGLTFA
                     VITAGCVLALGALARFQNVSHLAGQAPRWIETAFIVTALPVVAFHILVIVWNNAAALR
                     VSESMLAERAAQLAASRTRLITAADDERRRLERDLHDGAQQHLVALAVLIQLARTAGH
                     DSYQPLLTEASALVETAIAEIRRLAHGIYPPLLVSGGLAQALPAVAARAPVPAQLNLH
                     GLGRYPASIETALYFCCSEALQNAAKHGGPGTTVTITAHADDHTITLTINDTGRGFDP
                     ATIGTGLTNMTDRLSAIGGDLVIDTAPGRGTRVTAVVTTSVPPGSPELP"
BASE COUNT         3172 a         6080 c         6049 g         3043 t
ORIGIN      
        1 cgtccggcgg cgacgcggtc cacgccgagt tgacatcggc gacctggtgc gacgcctcga
       61 gttgacggac gaggtcggtg ccgacggcac gcgctgtcag actttcggcg ccggcctcgg
      121 aggtcacggc gatgaccaga tgcatgtcgc cctgaccgaa cttctcggac agcagccgag
      181 ccgcctgcga cgactcgctg gtcggatcct gtaggccgcc ggcggacagg ctcttggata
      241 ccgggatgcc aaagatgccg gcgccgacca tgaccagcaa tgcgaccgcg atgatccggc
      301 gtggcgccgc gatggcaagt cgagcgattc cttgtagcac gttcgtcttt ccgcgccgct
      361 ccacctcgac gcccagcgcc accccgggct gcgcgtcgcc gcggcttcct gcaagaggac
      421 tgagagtacg cacgatgcac ctttctagcg gtaaacgaga ccctggccga cgatggcgag
      481 atagaaaacg atgacgaaaa ccgttggcca acttcgggga tgccggtccg cgccaccccc
      541 gaggaagatg catgcggaga cgcagggcag gatgcccgcg gccagcggca cccaggccac
      601 cggccaccag tgctccgccc accgccacgg cgcggtgacc gacaactcga gcagcaccgc
      661 gagcatgccg aatgcccaag cggtcttcgc actggccgat acaccgatca gctgcgagta
      721 ggagtgcggg tcccccgcgc ccgtccggca cgtcttcctg gcgaattcca gatgcaggaa
      781 tgcgctggtg aacacgacgg gcacggcaag gaggcgtccc cacgaggcgt caccgacacc
      841 cggctggccc accatcacga acccggtgac caggaactgg gccgggcaga ccaccgcgag
      901 gttgacgatg gcgttgtccc gcacccatga cacgtgcaat tccatccacc agagcgcgag
      961 gccgtagccg agtgcggccg ccagcaccgc cgccgccgga agcgaaaccg ccgcggcgag
     1021 agccaaagcc actgcggccg tcacggccat cgccacccgc agctctgcgt gggttatccg
     1081 gccctgtacc agcggacggg tgggattgtg caggcggtcg tagtcgagat ccttctggtc
     1141 gtcgacgatc cgcaggaagg cgccggccgc gatcagggcc agcaccttca aggcggtgtc
     1201 ccaggtcggg cgccacgggc cggcggagcc ggtcactgtg cccgtcatgg ccgtcatgct
     1261 ttcgacggcg acgacccaca gcgtcagata gatcgggtag accgggaggt accgctcgac
     1321 cagaaacgcc cggattccgc cggtccgttc gatgaccgtc atgccgaagg cgacattccg
     1381 tgaccgccgt cgacggcggt gaatctcagg tcgaccgaag ccctcttctg gcccgactgc
     1441 atcacctcgc cgcggaacga caaagcgtcc tcagcggact gcgccacggt gaccgtgttg
     1501 gtggcgtcca attcggcgaa cgaatgaaag tcacaccgca gccaggccgg ccccgtgatc
     1561 gtctcgccgc tcgatcccag gaccgccagc gaaagttggc gacacgcctc gaggatcaag
     1621 cccccgggca gatggtcgag aggatggtcg aagatcgtgg gatggctgac gtccaccacg
     1681 atggccgcgc tcgccgcgcc accgtcaagc cgggccggct cgccgatcac cacgttctcc
     1741 ggacgtgtcc gcccgaccat ctgcggatcg gccggggccg cctgtggccc cgggtcccaa
     1801 ctcgcatcgc cacgaaacgc ctcccacttg gcgagggaca tccagccgaa tgccgccgac
     1861 agctccatga ccttcgtgcc ctcgtgcatc aggacggccg ccacgtcgag cccgtgcacc
     1921 gggccttgaa ggccttcgcc gccccgccgg aactccttgc gcacggcgat gtcgagctcg
     1981 agatccagca cgtggggacc acacttcagc gcggccagat caatgacgga cagctcgtaa
     2041 taacgcacgg tgtaccgggc gtcgagaggc acgccgtagc aggcgtgact cccggcgatg
     2101 cacgcctgcc ggaacgcctc caggaccagt aacgggtcgt gataggcgct ctgcacgccc
     2161 gtcgtgtcgg tgtgaaagct gtgtcggcgc ggcagctgcg catggcagat ccaggtcgcc
     2221 ggagtcgttt cgaccgacgt gatgagtact tccgcgatgg agcgcttgtg taccagctct
     2281 cgcgcgatgg ttcgggaata gttgccagcg ttcataattc acctcgttga gtctgtcggt
     2341 gcatggggag ataggcggat tcgaggacga tgcggaacca cgggtcgacg gggacgtggt
     2401 gactgtcggc cagctcgatg gccgccaccc actccggtcc cgtccggggc cgcgccgtcg
     2461 cgcctgacgc ccgcaggtgc tgccagacca tgtcgtgcgt gaccgccagg tcgccgctcc
     2521 ggcgctcgac gccggattcg atctggtcgg ccagtcgaac ggcgtcggcc gaggcgtcgg
     2581 tgccggcggc acacgcggcg atgacctcgc tgagttcggg gtcggcgaag tagatgctca
     2641 gctcgcgcaa gacgtcgtcg ggatcatcgg gcgtatgaac ctggttgagc aggtacgttc
     2701 cggcggccag cgcgtacctc agctggccgg gcgcccgctg cgccgggtcg gcggggccct
     2761 tgtccgccgt caggcgaacg gacgccggca cgctgtcgtc gggatgggtg accaccagca
     2821 cgatcgatgc ggacatccca gcgagccggt cggccagccg ccgccgctcg ggagttgcgc
     2881 tgtgccagtt gaagtaccag agggcgcgta gatgcaggcg gctgagcccc actggttgcg
     2941 ctcccacgat gcgatatccg tgctcagtca gccaggtgac ggctcgccgc attccccccg
     3001 tgacggccgc gtcgggtttg aggagcagca gtgctgtgcg gcgggcgaat tcatcgggat
     3061 cgatggacag ccgctgcagc gcgtcgcagg tttccaggaa ataggtatcg gatgcgtgcc
     3121 tactcgggct ccgccggtgc gtctcgggtg gcgccagtgt ggcgacttcg gtgcggatca
     3181 tgcacaattc ctctctcgtt tcagtgcggc cagcacttcg gcaatggatg ggcccgatct
     3241 gcccagataa cctgtgcgca ggcatgagtc gagaattcgg cggacgtagc ccgcgccggt
     3301 tgtgggcgca gcgatgccga gccggtcacg ggtgttcgag ctgtcgaact ccagccggcg
     3361 ggtgaggtag ctcgcgtaca acgtcccgac cttctggaaa tacccgcgct cgatctcgtc
     3421 gagttggtcc acgctgaacg tgctcggcgg cacgaagtcg gccacccgaa acgatgggta
     3481 ctcggccaag atgtcggaaa gcgtttggag cgagacacat ttcgcgccga ctgcgtggaa
     3541 ggtgcgtccg acgttgtcgg agaacctctc ggtcgcggtc gcgatggtgt ctgcgacatg
     3601 tccgacgggc gacagcgcca acgtggccgc gtagtttccc ggcagcgttc gcaacttgcc
     3661 ctcgacgatc agtttgagca cctgatagat gtgtttgtgt tcacgactgt gcccggtgcg
     3721 gtgttcgccg gagacgatgc cgggacgcac cacggcccat cgctgccccg aggcacgcac
     3781 cagctgctcg gcctggaact tgctgcgctc gtagccgttg ccgaaccgtt gcccgacgtc
     3841 gagctgatgt tcgccgaaag tcccgttgaa ctcaccacag acgtaggcgg tgccgacgta
     3901 tagcaatccg gtgtcccaat tccgtgccag cgcaagcgcg ttggcggtac ccgccacgtt
     3961 cagctcggcg taccgctggt cgggcaggcc gaagtccgtc accgcggcac agtggacgat
     4021 caccgcgacc tgcgccgcga gctcccgcac gacatccgcg tcgagcccga aatccgggcg
     4081 ccggacgtca ccgcggagca cgcgcgccgg ccggaccgga cgcccgttgt tgctgacgat
     4141 gtcgaatcgg ttgtgcacca aggcgacaac gttgtggccc cggacgacca gccgggccac
     4201 gacttccgcg ccgacgtgcc cggccgcccc ggtgacgaga atcacccgcc gcgcgttcat
     4261 gagcagttcc tctgcgtccg aacgagattg accagatcgc cgactgtgct cacgccggcc
     4321 agcatgtcgt gacccagcgg cggcagtgcg aaggcatgct cgacggcgat gcacaattcg
     4381 agcagccgca gcgacgtata gccgaggtca tcgatcaacg cgcggtgcaa gtccggctcg
     4441 gccgccggag caggagccag cgcggtcacc agcgccagca cgtcgttctg gattgcagtc
     4501 atgccgattc tcccgattca gagtccgatc gggcggccct ggccgccggt tgagttccgc
     4561 cgctggacaa caggtcccgt gcttcgtcca gttccagata ggcttcgagg tatccggcca
     4621 tccgtagcca ctggtagccg tacctgaggg tctcccgcca ctgttccggc gaggcgaacg
     4681 acgaggggaa catctcgccg ccgctcgcgg cttggacgtc cgacacgaac cggtcgatct
     4741 cggcccgggc gtcaagtgcc tcgtcatcat cgtggacgga caggcgcacg aggcgccgag
     4801 cggtcgccgc cagctggggg tgctcgggta tgagcgtggc gaggccttcg acgagcaccg
     4861 gatcacccgg cagcggcgtg acgccccacg cgctcgtcag gatccggacg gccatggtga
     4921 cgatccggcg cccgcacagc gatgccacct gccactgtcc gtccttgacc gcaccctcga
     4981 agtcttctcg ggcgttctcg agcaccatgt tggccagcat caacgcaccc gcacactcgg
     5041 caaatggctt tgccgccagc ggggatcgag cgatgtgccc atcggcgatc gcgccgttga
     5101 tcgtgatcac cgggcgacgg gtggagggcg ccggggtcag cggccgactc gggtcgcaca
     5161 tcgcggccac cggcctgatg gttcccgcgc cacgccaacc gagtgcgatg agaccgtatc
     5221 ggacgaacag cgcgatgtgt gtgctcgccg cccgggtgtc ggcgagcgtc tcgccgaaca
     5281 ccacgtggcg ccaggattcc gcgcactccg gtgagagcac gaacacgtcc gccttgccgc
     5341 gtacgacatg tgtggtggac ctgatcggcc aggtggtcac gccggggacc cgtcgcaaga
     5401 tcaccttcgc cgggtcgagg gtggccgtgg gcgcacccag tctggccgaa agcgccagtg
     5461 cgcgaacgac gaaatcccgg gctccgacct cggcggtctg cgggtagtcg aggtccagat
     5521 actcggcgag cggggccgcc acgtcgcgat caccgcgggc gcgcagccgc tcgacctgca
     5581 gcggaagcca tttgacttcc acatagcctt ccccgcgctc ggcgaggaac gccttcatcg
     5641 cctgcgtcac gccttcgcgc gcccagtgcg ctgcctcgcc gtcgtgatcg agaagggcaa
     5701 gggcggcggc ctggcgcaga cactgcccgg cccgccgacg ccaccacgcg gcgagcaggt
     5761 tcgtcagttc gggataggac acgacgtcgc ggagctctcc gtaccgcggc ccgatgttca
     5821 gcacggtgcc ccgaaggaat cgttggaccc tgttgagcag gtcctcggtg attccgctca
     5881 gcgaacccgt gtcggcggcg cggcgaatcc tttgcagccg ttgacgtact tggtcgtgtg
     5941 actggacacg gatctcgacc cggtggccgt ccacgaagag aaccgtaggc atgaccgctt
     6001 ctgtgccggc gcctggcacc aacagcagca ggtcgatgtc ggagccttcg ttgccgaaac
     6061 cctcggccag cgaaccctcg aaatgcactg tcgcgtcgtc ggggatcacc gccagttcgc
     6121 gggtccgctc gaacagcgaa cgcacctttt cgagcagagc cggatcacgg tccggaccgg
     6181 acatcgacag cagtgcggcg ccgacgagat aacccgctcg ccactgctgc cacatgtgtg
     6241 cgcggcgggg ctttccgctc gaggttcgcg acaggccgcc gcgcggaaca ccgatgaacc
     6301 acaccagtgc gtcctccccg acctgagcct gcactgcggt gcgagcggcg gcgatctgcc
     6361 cggcaaggag ttgacgtgcc cgctgctcga cgaacagggc aacccccggc cggccgctct
     6421 cgttgaccgc ggcggcgata agccggctcg gtgccacgcc caacgcggcg gccgccgtca
     6481 cgtcgagatc ttcggcaaag accgtgcggc cgttgacttt caggctcgtc cccatccggc
     6541 cgagcacgaa caaccgcccg tccaggatga acccggcgtc accggtgtgc aattcgccgt
     6601 cgatgtatcg tgttgcgctg tccgggtttc cgcgatatcc gccgaacacc gatggtccgg
     6661 acaggacgat ctctccgaga atcccctccg cggctgccaa accggattcg tgggcgatcc
     6721 gaacgtggtg gcccgggatc ggggcgccga ccgacatcac ccaatcggtt ccggattcga
     6781 ctgtcaagca gtcggtggcg gcggccagga cacgcaccgg ttgcccgtga cgcaaggcgg
     6841 acttgtcgat ccggaccatc cggtgcggtt cacccacgcg gtgcgcggtc gccagcagcg
     6901 tgtgctccgc gagaccatat gacggcagga acgcgctggg cgagaatcct tgagcggcgg
     6961 tcagttcggt gaaggccctg aggtggtccg ggttgatcgg ctcggcgccc accaccatcg
     7021 tgcggatccg cgagaggtcg ataccctcga tgtccttccg cgtcagtcga cgcgcggaat
     7081 acgcgagccc gaatgacggc gagaccgtgt ttcgtgcgag cgcggccacc cgcagccaac
     7141 gagccgggtc gcggacgaac tgatcgggac gcatcaagta cagcacccgc tgccgggtga
     7201 tggcctgaaa gatccccccg atcaatccca tgtcgtgata cagcggcagc cacgtggcgg
     7261 tggcgtcgtc gtccaccgtc cccgtctctt cgcatgcccc gtcgatggcg gcgaggttgg
     7321 tcgtcaggtt gcgccaactg atgcgcgctc cgcgcggcag gtgcgtggat cccgacgtca
     7381 tctgcagtag aacaaacggt gcgggagagg ccaattcgat caggggcggg tgtgccggca
     7441 caccgtcgag gacgatcgtg gcaggcgctt ccggcaggtc ggcggccgcc cgcgccaccg
     7501 cgtcgacgaa ccccgacgag gtgaccaccg cactggcctg cgagctttcg agaaccccgc
     7561 gaaggtgccg ccggtactgt tcgtgactgc cgaacagcgg gggcgcgatg ggcgtcaacg
     7621 tcgcccccgc cgccagcaca gcaaacatcg tcgccaggca caggtgcgaa gtcggcatca
     7681 gcacgcacac ggtgtccccg gaaccgactc cttgctccac gagcagacgc gccactcgtc
     7741 gaacttgttc ggccagcgtc gcatacgaaa ggaattccca gccgccgtcg tcggccgcca
     7801 gatgcacgcc ggtgtcctcg ctcggtgagg tcagccaccc gaggatgcgg gcgacatcgg
     7861 cctcatcagg ttcacgcatc gaaactcccc agttcgggct ggtcttccag cagggtgatg
     7921 cggccactgg caccgtccac gctgacggtc atgccggtgc ggatcgcggt ggtcaaaccg
     7981 tcgatctgaa cgacggtcgg cacgcccagt tcgcgggcga tgatcgcgac atgggtgagc
     8041 ggcgatgcgc gttcgatgag cagcgcggac gcgaagggaa gtgccgccac ccaaccggga
     8101 tcggtgcgat aggccaccag gatgccgctg gagaagtcct gcggcttctc cacgaccacc
     8161 gcgcgtccgg tcaccacccc ggggcaggac ggcgtgccgt tgagcaccgt cccggccggc
     8221 gcctgtgtct gatcgccgga caaccgccat ccggccctcg tcagttcctc cggggagtag
     8281 cccactcccg tcgtggcgaa ccgggcgggc gccgtgagct tccggtagcc ctcgtgcagg
     8341 actgcgcgac gggcgatcac ctcgcgcgcc gccccgcggg acatcgagcc ctcgtacacc
     8401 gcgaacaact cgtcgagacg caacaggaag acatcggcgg acgcgtcgac gatgccgcgt
     8461 tgctcgaggt cgcgacccat caccgcgaca agggatttca ggatgccgaa gccctgggtc
     8521 cggcagaacc gcagatgctc gcgatgggtg gccgcgcgct ggattttcct gcgcagcgca
     8581 tcgaagagcc tccggcgggg cccgcggagg tgcgcatcga ggtatgcctg cacggaagca
     8641 cccgaatcac ctgcacgctg tgtctcttcg gcggaagcca tccgcagcat gtggaagatc
     8701 ccggacgggt cctgccgcag atcgggcgcc tcgagcttga gctcgtcgag gcaacggtaa
     8761 ccgaatctgt cgatgtactc gtcgatcttg cccagcaggt cctgccagtg cggggcaccg
     8821 gcagcgctgg cagcaccggc gtggtcgacg agtagggggt aggcgaagtc gggctcggcc
     8881 gcgtcgatga acgcccacag gtccgcgtgg tcgcgcgcga atgcggcgat gtcggcgagt
     8941 gctcgcgccg gctcgatgga caccacgtcg gtcccgggat tgacgacggc gaagccgaac
     9001 cactcgggtg cgtggggcag gaacgtcttg gtgagggcag cgagggcgcc caccgaagtc
     9061 agcaggatgg cgtcgagaac catcatcggt ccccagatat cggccacgtc gtcttggatc
     9121 cggcgaaaga gccggtacgc ctgcggaccg tcgagcgcgg agacatccgt caggccgtga
     9181 cggtcgacga actcctcgaa cgagctgtcg aagcgggcga ccaacgcatc gatggaccaa
     9241 aaccggcggg cataggtgac ggtggtgcac gcccgagagc accctttgac gagcgcgttg
     9301 gagaatccga acggccgcag ggcccgcgct gtcctgcgat cgaggggctc actgacgccg
     9361 agggcgactt ccagcacacg ccggttcagc ggatacccgg gagcgatgcc caccatgcgg
     9421 taccagtgca acaggttgta gtagacgtga ccgttgaact ggcccagcat gaccggcagc
     9481 caggactcca tctgatcgag ctgcgccgac ggcactttca ggctgcgggc ataggcccgg
     9541 taaacgtcgg cgtacagctt gcgagcggtg gtgaacgtca ggggcgaggt gacacccgcg
     9601 aagctctcga tgatgttcga gttgtcccag atgcgaagat cgccgggcgg cacgtcttcg
     9661 ccgagatatt ctgccccagc aggcgtggcg gggttttcgt tggtcagtgt ggtgataggc
     9721 cgggactgca gaatccacgt gttcgactcg tcgacagccc actcgatgtc ctgaggagca
     9781 ccgaaatggt gttccaagcg tgcgcccagg tcgatcagcg agtcccgcag gtcggggccg
     9841 atgctgcaat gtcgttgttg ttcggcagga acggccaccg tgcggacgcc tccaccgagg
     9901 ccggtgacga tcatctcctc cttggcgccg accttttcgt cgaccgcctt gccggtgcgc
     9961 ttgtcgacca cgacggagtc tgagtcgacg gcccccgaga ccagaccctc gcccagaccc
    10021 aggacgccgt tgatgacgat gcggtccggt gaacccgtga ccgggtccgc ggtgaacatg
    10081 acaccgctgc tgcgggcctg cacaagccgc tgcacgatga cggcgagcgc aaagtcgtca
    10141 atgtcgtgca agtccagccc tcgcatgtgc cggtaccgca cagcccgttc tgagaaggcc
    10201 gatgcccagc atcgacggac cgcgtcctcg accgcgacgg gtccggtcac attgaggatg
    10261 gtgtcgaaga gtcccgcgaa cgaatgtgaa gcgccgtcct cttgccggcc cgacgagcgt
    10321 acggcgacgc ttgtgttgcc cacgtagtcc agcgcagcgc cgatcgcgtc caggattgtt
    10381 gcgggaatgg ccaagtcgcg cacgaccgac tcggcgtgcg ctgcctcccg cagccgttcc
    10441 gacaacggca gcggtgcgtc actcagctgc agaccggccg ccgcgacggc ggcgtgaaag
    10501 gcgtcagtgc ccagaaccgc ccagcgcggt accgggaagc cggcggcggt gagggtgtgg
    10561 agattgtggg ccttgccgcc cgcgagggtg cgcagctgat cgccgggggc ttgatcggtg
    10621 atgagaaatg gcattctgcg tgcggtgcgc atcgcttctt tcctggtttg tcgcgggttg
    10681 ggggttctcg ccgctaagac gggccctgaa actggcttgt ggaaactcct tgatacacat
    10741 caagtttcgt cggacggcca gcggtgggcc agagagcaca caccacacct gccggtgatg
    10801 tacacacccc ggtgtgctca ccagcgcaac gtggtaccta ccggcgggcg gactccaccg
    10861 tcatcaccaa gagtcgcgtt cggatgtgaa ttgatggtct tgacgaaccg catcgaagtg
    10921 agcgcagcgt cgcgagcgag gtcggcgagt cggctggcga cgttcgagct actcagcgtt
    10981 acgttcgcta ggcagggtcg cacacgagtc gggcagggcc gctccgcatt atcgagaaga
    11041 gctcctcacg cgatctcgcg ccgaggcgct ggtaggcgcg atagatatgt ccctcgacgg
    11101 tgcgaaccga tgtgacaagg cgctccgcga tctccttgtt ggacaagccg gcagcggcca
    11161 gctcgatgat ctcgcgttgg cggtaggtca atggttggtc cgccgggttt ctcagcgcgg
    11221 gcgtgcacag cccgccacac tcgttggcca actgtgcggc caacgctgcg gcgtagagac
    11281 tccgcttacg ctgctggctc cggctgaacg ccatgctcgc ctgagccgcg acatcggcgg
    11341 ccgtggcgac atcgcccagt gcccggtagt cggccgatgc cgcgagcagg ccctccccac
    11401 tgccatcccg cagcgatttg gtgtggcgag caacggcatt cgcaagggga aggttcaaca
    11461 acccggccag ttcgcgcgcg cgcttggcac ctgaggtgtc accccattgc gccgctacct
    11521 ggaggcaagc caattcgtgc gtcggccggt taggctccca cgcacccttg ccggccgcct
    11581 gaacaaccgc gatcgcctcg cgcaggcggc cgccagcggc gagcgcccag cctctcgcca
    11641 tcgccagagg ggtctgcatg aaatggtatt cggcgggcac agaccgccga acctcggcga
    11701 tcgcgtcgtt ggcggcctcg gcatcgccca gtttggcgtg cgcctcggcc aacgcgaaac
    11761 agatggccgc ctgtagacct gttgtgacgt cgtggttttc cattcctgcg agcgcctcgt
    11821 gcagaagttt gaccgccgtc ggcacttgcc cgcgcgcgag ttcggcatag ccaagcaaga
    11881 atgccagata cgcagcgaca acaccgggaa catcaggggc cgagccggcc agttgcgtgg
    11941 ccgagcccac acattcgtcg atacggccgg tcaagcggca cgcctgtgcg tagacagccg
    12001 cgtaccagaa cctcatgggc gacgcttgaa acgaggtggt cgcgcggtca atgtactccg
    12061 cagcatggtt ggtgacgtcg tcaatttcac ccagcgcgcc cagcgccatc gtcagtgcgc
    12121 tggacgccag catcacgtga aaatccggca ccgtcgacga actcaacgct gcccgagctt
    12181 tttccacggc agtctcacag cgggccgcca cggcgtctgc gcaggcctcg accgcgacgc
    12241 gctcggctcg ctccgccgcg gattcccgcg ctgaggccaa gccgtccaga atggtcgacg
    12301 cctccgtggg acggccgaga ttccagataa ggttcgcggc tcgcacggtt gcccagtggt
    12361 gcccgtcggc cccatccatc ccgatctgac gcaggatcgc atcggccccg tcgccgtcac
    12421 cccgggccgc caggctcaat gcccgcaggc ctgtggcctc ggccgcgccc gcgtcggcag
    12481 cggcggtggc gaaccggtca gacaagtcca ggtcgagcaa cgtcatgccg caccgagccg
    12541 cctccaggaa tagctcgcgg tcgggggcca agtccgattc gagggtgagc aatgcccgcc
    12601 gcaccgttgc ctgcatgtcg tcgtcgtcct ctttggccaa ccgctgggcc agcttgccgc
    12661 ggatccgcga cagatacagc tcaccggccg tcgcgcgtcg aagttcaccc aagagcgggt
    12721 gtgcaaggcg cgccatgagc ctagagccgc agcgttccac ggtaacgagg ttcatctgtt
    12781 cggcaacctc gaggtcgtgg cggcgtacca ggtcgcagag catgtccacc tcaagcggct
    12841 cgtactggga caatgtgtcg acgaccatgg caacatccgg ggcgagccga cacagttggc
    12901 gtccaaccat gccgctgatg ccgtccgacg ccgcgacacg ctcgtcccac atccacaccc
    12961 cggccacctg ccgcatccgg ccggccgcga tctgatcctt gagcagttca cgcgtgaaca
    13021 acgcgttccc gccggtcagc ttccaaaacc gctgggcgct agcgctgtcc accgcacccc
    13081 caagagtcgt ctcaataatg agacgagtat ctgccgacga aagtggttgc agatcaacgc
    13141 ggttcagcag atgatctttc cacagcgcgg tgatcgcatc gggctgatcg ccgccgctgc
    13201 gtacggtgac cagcagtcgg gtcccgggca tttgagcgag ttggtgcacc acgtgagccg
    13261 atgatccatc gaggaggtgc gcgtcgtcca cgccgatcag cacccggccc tggcgctgtt
    13321 gcgcgacaaa ggaactgata acgcgtcgga cgtcggataa cgaatcgatc atcggttggc
    13381 caagcgatgc agcgaaagcg cccaagggaa gtttgcgcgc cgactcggtg ccgacaatcc
    13441 agctcgtgcg cgcgcctgag gcttcggcgc cggcgaggat ctcacgcgcc agccgagtct
    13501 tgccgacccc cgccgcaccg acgatcaccg cgccgcgttg attaccagct ccgctgagtg
    13561 cgcgccggat ggcgctcagt tcgccatccc gtccggtcag cgggccgccg gtggccgcct
    13621 cccttgcggc gaacgcgatc tcagctgacg ggttggcgca ggactcaaat tcaggcccga
    13681 aggcccgcat ctggcgcgcc accgccgggc gtgtgaaacg caaatggtcg cggcaagcgc
    13741 cgcccgatgc cgagacttcc gcgatcgtct ggtcacgacg catcgaatca gcccttcatg
    13801 gcttcgagtg tggtgcgcat cgcttctccg ttggtttgtc gcgggttggt ggttctcgcc
    13861 gctaacacgg gcctcggtgg aaactccctg atacacatca agtttcgtcg gacggctaga
    13921 tggtgggcca gagaggacac accacacctg gcggtgatgt acacaccccg gtgtgctcac
    13981 cagcgccctt gagggagagg atccacacga tggtgcaacc tggccctgca cggagctctt
    14041 ggccgtcaag ccggcaccgg tcccgatacg gtcgcaggac agttaggcgc cgtggtggct
    14101 ggtcgccgtg ccagttccct ccgggttcgt ttcagcagag cgtcgagtct gtcgttgacg
    14161 acgtcaggac gatcgagcat caacatatgt cccgcaccgg ggacatcgac aaactctgcc
    14221 gcggagaccg cagcggcaat ggccgccgaa tggttagacg gcgtgacccg atccacggtt
    14281 ccgcacatga tcagtgtcgg tgtgcggctg agtgcccggt aagccgctgt gccgtcgaag
    14341 cgagagatcg agccgagaaa acccgccacg attggcagct gcggcgcgct gatgacggca
    14401 gccagggtgg gcgccttctt tgtagcgagt cgccatagtg gttccgtggc acggactgca
    14461 gtgatgcgga cggcatcgac ggaggcgggg atgtgggcgg ccagagcaca ggcaccgccg
    14521 gctacgggag tagtcagcaa tttgccgagc ccgtagcgtg tgatgtccgc ggccgcggtg
    14581 gcgatgagcc cgactcccgt gattcggtcc gtgttacggg attggtgatg ctgcaggtac
    14641 tgcaggatgg tcatggcccc catggaatgg ccgacgagca cgatgtcgcc ggcgggcgtc
    14701 gtcgcttcga tgacggcgtc gaggtcacgg gcgagctgcc cgagggtgtt acgtaggaca
    14761 cagccgcgac cggagcggcc gtgtccgcgt tggtcgtacg cgattacccg gatgtcaggg
    14821 tgacgcgatg tcagatgact cgtctggcgc cgccaactat ccccgtcgcc caggagtcca
    14881 tgcaggagaa tcactgtcag atccgaaact ccgggaccaa tttgtctcgt tgccagcgca
    14941 agcccgtcgg ctgtggtgac tgaaacccgc tgtgctgcta ctgggttcat cagcgtggcc
    15001 agccactaat cgttgaaatg ccgctcgcgg tcccagcgct caagccacaa ctgcaggtcg
    15061 tgcatgccga ccgcaaacaa cgccagtgac agcacgataa cgacgttctg gaaggtactc
    15121 acagccggtc catttcgcgg gccatgcggg agtgctcgag gtaactgatg cgctccgggt
    15181 attggcgtcg cgccggggga cggtgcaacg cggcgccgac aacttggatc gcgtcgatca
    15241 accggcggga gatcgacgcg ggtcgcggcg agctggccgc cgcagtgctg aacacaacct
    15301 cctgcgtggt gatgaaaacg ggcgcttgag acatcctgtc ttgcaagaag gttggttcag
    15361 cgtcttggat gacttcgggc gtcgccgcgg atgtcgttgg tgattgcgac atttcgcctc
    15421 ctcgtcgtgt tggcttgcta ccctggctgc acatgctctg ataggcatcg agtgtcatcg
    15481 gatggctggc ggcgggccag agagcacaca ccacagttgg cggtgatgtg cacccccggg
    15541 tgtgcgcatc agtaccgaag gtggtgcgta cctaccgccc tgcccgagcg tcgcgcagga
    15601 cgctcatccc ctgtgcgcgg gcgtttagct gttgacgctc gcagcgggtc gcaacatcag
    15661 ggtggcgatc ccggatgtcg gcatcccaaa tgccgccacg agaaacgcaa acctcgccga
    15721 cgaagtcgac gcgttcgagg gcggcaaacc gccccgcagc cgatggcggg gttgcgtcac
    15781 aaaactaggt agctaactac gctagcggac cggcgaagct acgcgaatag ttgcggatag
    15841 cctcagtggt caacaccgac gctttcgagt agcgatcgtg aacgaccggg aaaggatggc
    15901 attagggtga gtggtccaat ttcggtcctg attgccgagg actgcctgct ggtgcgagac
    15961 agcgtttacc gggcattgtc gaccgacgcc gatgtgaccg ttgttggcgt tgccgaggat
    16021 tatgactcca cggtcgaact cgtcgaaaag catcaaccga cgatgctggt caccgacgtg
    16081 cggatgccac cgacatcgac cgatgagggc attcggttgg cccggtggat gcggtcggcg
    16141 catccggata ccggggtgat cgtgttgtcg caatacgcgg acccggccta cgcggcgggg
    16201 ttgctggatg gcggtacggc gtcacgcggg tatctgttga aggaacgcgt ttcacatttc
    16261 gaccagctca gcgaggcggt gcaccaagtc gcggccggtg gcacggtgct cgatccgctg
    16321 gtggtagagg cgctgctggc ccagccgcgg tccgccgcga cgctcagccg attgaccccc
    16381 agggaacgcg aggtgctcgc cgaattggcc accgggctca gcaatcgcac ggtggcgcgg
    16441 cggcttgtgc tgtctcagcg ggcggtggaa aaacacatta attcaatttt tgccaagctc
    16501 ggcttgtccg tcgacgattc ggttgatcgg cgggtcaagg ctgtgctgat gttcctcgac
    16561 gacgtatcgg tttagaggta tcggtttaga agtgggtgtc cagacccttt aagaagtgtg
    16621 gtgtgtgcac catggtgtgg ggcaaaaggg gctccgcaca atgcgcacgt gaggcacgag
    16681 cgggtgcgag tttgggtcgt cgacgaccag gccagtttcc gtctcgctac cgccgccacc
    16741 gtggctgcaa cggaggagtt cgtcatggcc ggcgaatgcg agaccggtga atccgcgatc
    16801 gaactccttc gcgacagtga tgccgggatc gtgttgatgg atatccacat gcccggaatg
    16861 ggtggcatcg aagccacgcg gcgaatccgc gccgcacacc cagaattgat ggtcctgctg
    16921 atgtcaacct acgacctcga ggaccttccc ccggcggcag ccgattgcgg cgccgcgaca
    16981 tacctgcaca aagaacacct gagtccagat ctgctgaccc ggctatggcg aacagcccac
    17041 tgaggccgac gacggcgccc ggatcggtgc cctactggcg ggtgccgaag gtcctccttc
    17101 tcgcggcggt ccggtcttca tcgttgttag gtcacgacct cgttcaccgc cttacggcgg
    17161 aacgctttca gctgttcctt gctggttacc tggccatgta cttcggtact ggcgtgttgc
    17221 tgccgctgtg ggagtaccag tttgtgctgc gatcccgctg gttgctggcc attgttgggg
    17281 tctgcatcgc gcagtgtgtc gtgctggtgg ttgcgctgcg cctggcgcga cgcaccaggt
    17341 atcagcaatc gatcacgctg gtttgtatcg gtaactgggc gagtgttttg ctcattacct
    17401 tcgtcgcgcc ggtcctgttg ccggtgatgg tgctcgcggc gttggtgccc gtcgttttcg
    17461 cccagcccta cgcccgctgg cagcgagggt tgactttcgc cgtgatcacc gccggctgcg
    17521 tactggcgct gggtgcgctg gcccgctttc agaacgtttc acacctggcc ggccaagcgc
    17581 cccggtggat cgagactgca ttcatcgtca cagcactacc ggtcgttgcc tttcacatcc
    17641 tggtgatcgt gtggaacaac gccgcggcgc tgcgagtatc ggagagcatg ctcgccgaac
    17701 gcgccgccca actcgcggcg tcacgcacgc ggctgatcac cgccgccgac gacgaacggc
    17761 gccgcctcga acgcgacctg catgacggcg cccaacaaca cctcgtcgcc ctggcggtgc
    17821 tcatccaact cgcccgcacc gccggacacg acagctacca gcccctgctc accgaagcct
    17881 ccgcgctggt agagaccgcg atcgccgaga tccgcagact cgcccacggc atctaccccc
    17941 cactgctggt cagcggcggc ctggcccaag ccctgcccgc agtcgccgcg cgcgccccag
    18001 tccccgcaca actgaacctg cacggcctcg gccgctaccc cgcctccatc gaaaccgccc
    18061 tgtatttctg ttgcagcgaa gcactgcaga acgccgccaa acacggcggc cccggcacca
    18121 cggtcaccat cacagcccat gccgacgacc acacgatcac cctcacgatc aacgacaccg
    18181 gccgcggatt cgaccccgcg accatcggca ccgggctaac caatatgacc gaccgcctat
    18241 cagccatcgg cggcgacctc gtcatcgaca ccgcccccgg acgcggcacc cgcgtcaccg
    18301 ccgtcgtcac gacctcggtg ccaccaggca gcccggaact gccc
//
LOCUS       DASSGQ010000081         3302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1190753,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000081 DASSGQ010000000
VERSION     DASSGQ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190753"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VFI55_03670"
     CDS             <1..713
                     /locus_tag="VFI55_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7073166.1"
                     /translation="RVLIDELVRRSRSRWVRATVDRLERAGHGWALLDDTGAHWEADA
                     VILAVPAPRLARLVPQITPRTCAAANQIASASSVVVALAVPAATAFPQCSGVLAASGE
                     PLRAKAITLSSRKWGLRGDAQLLRLSFGRFGDDLAATASDEELLTWALGDLAAVFGLA
                     VDPVDVRLQRWIDAMPQYGPGHAGVVAELRAGLPPTLAVAGSYLDGIGVPACVGAAGR
                     AAERVIRAVEGSEKQVAR"
     gene            719..1426
                     /gene="hemQ"
                     /locus_tag="VFI55_03675"
     CDS             719..1426
                     /gene="hemQ"
                     /locus_tag="VFI55_03675"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HET7073167.1"
                     /translation="MAKLDAKLDFAALNSTIRYLMFSVFSVRPGVLGQERDAVIDDAS
                     TFFKQQEERGVVVRGLYDVAGLRADADFMIWTHAERVEALQATYADFRRTTVLGRACS
                     PVWSSVALHRPAEFNKSHIPAFLAGEEPGNYICVYPFVRSYEWYLLPDEERRRMLAEH
                     GMAARRYKDVRANTVPAFALGDYEWILAFEAPELDRIVDLMRELRATDARRHTRAETP
                     FFTGPRVPVEQLVNSLP"
     gene            1440..2192
                     /locus_tag="VFI55_03680"
     CDS             1440..2192
                     /locus_tag="VFI55_03680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7073168.1"
                     /translation="MTTQFDPTDPTRFEEMYRDDRLSHGLPAATPWDIGGPQPVVQQL
                     VSLGAIKGEVLDPGTGPGHHAIYYATKGYSATGIDGSEGALERARENARKAGVSVKFE
                     LADATKLDGLDKRFDTVVDCAFYHTFSTEPEMQKSYVQALRRATKPGARLYMFEFGAH
                     DVNGFTMLRSLSEADFRDVLPLGGWEITYLGPTTYQVNMSVETIEMMAARNPDMADEA
                     ERLLERFRAMEPWLVNGRVHAPFWEVHATRVD"
     gene            complement(2362..2772)
                     /gene="msrB"
                     /locus_tag="VFI55_03685"
     CDS             complement(2362..2772)
                     /gene="msrB"
                     /locus_tag="VFI55_03685"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET7073169.1"
                     /translation="MSRPKVQLSDDEWRKKLSPQEFDVLRRAGTERPFTGEYTDTKTQ
                     GVYQCRACGAELFRSTEKFDSHCGWPSFFDPANSDAVILRPDHSFGATRTEVLCANCH
                     SHLGHVFAGEGYPTPTDQRYCINSVSLRLVPAEA"
     gene            complement(2809..>3302)
                     /gene="aftC"
                     /locus_tag="VFI55_03690"
     CDS             complement(2809..>3302)
                     /gene="aftC"
                     /locus_tag="VFI55_03690"
                     /EC_number="2.4.2.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arabinofuranan 3-O-arabinosyltransferase"
                     /protein_id="HET7073170.1"
                     /translation="NSSVEGNGVYFGLPTWLIVFLRLLFTVLAIGAMWLLYRYYRTRD
                     PLFWFTSSSGVLLLWSWLALPLAQGYYSMMLLPFLMTVVLPNSLIRNWPAWLGVYGFM
                     TLDNWLLFNWMRYGRALQYLKITYGWSLLLIVVFTVLYFRYLDAKAENRLDSGLDPTW
                     LRG"
BASE COUNT          557 a         1085 c         1140 g          520 t
ORIGIN      
        1 accgggtgct gatcgacgag cttgtgcggc gcagccggtc gcgctgggtg cgtgcgacgg
       61 tggatcgcct cgagcgtgcc ggacacgggt gggcgctgct cgatgacacc ggggcgcact
      121 gggaggccga cgcggtgatc ctggccgtcc cggccccgcg gctagcgcgc ctcgtaccgc
      181 agataacgcc gcgcacgtgc gccgcggcga accagatcgc gagcgcgtcc tcggtggtgg
      241 tggcgctggc cgtgcccgcc gcgaccgcct ttccgcagtg ttccggtgtg ctggcggcca
      301 gcggcgagcc gttgcgcgcc aaggcgatca ccctgtcgtc ccgcaaatgg gggctgcgcg
      361 gggacgcgca gctgctgcgg ctgtcgttcg ggcgcttcgg cgacgatctc gccgccaccg
      421 cctcggatga ggagctgctg acctgggcgc tgggcgatct ggccgccgtg ttcgggctgg
      481 ccgttgaccc ggtcgacgtg cgcctacagc gctggatcga cgcgatgccg cagtacggtc
      541 ccggccacgc gggcgtggtc gccgagctgc gcgccggcct gccgcccacg ctcgccgtgg
      601 cgggcagcta cctcgacgga atcggcgtac cggcgtgtgt cggggcggcg ggccgggcgg
      661 ccgagcgcgt gatcagggcc gtcgagggct cagaaaagca agtggcacga taggtttcat
      721 ggccaaactc gacgcgaaac tggattttgc cgccctgaac tcgacgattc gctacctgat
      781 gttctcggtg ttctcggtgc ggccgggcgt gctcggccag gagcgggatg ccgtcatcga
      841 cgacgcgtcc accttcttca agcagcagga agaacgtggc gtcgtggtgc gcggcctcta
      901 cgacgtcgcc gggctgcggg ccgacgccga tttcatgatc tggacgcacg ccgaacgggt
      961 cgaggcgctg caagcgacct acgccgattt ccggcgcacc accgtcctgg gccgggcgtg
     1021 ctcgccggtg tggagcagcg tggcgctgca ccgcccggcg gagttcaaca agagccacat
     1081 cccggcgttt ctggccggcg aggagcccgg caactacatc tgcgtgtatc cgttcgtgcg
     1141 gtcctacgag tggtacctgc tgcccgatga ggaacgccgt cgcatgctcg ccgagcacgg
     1201 catggccgcc cgccggtaca aggacgtccg tgccaacacg gtgccggcgt tcgcgctggg
     1261 cgactacgaa tggatcctgg ccttcgaggc tcccgagctg gaccgcatcg tcgacctgat
     1321 gcgtgagctg cgtgccaccg acgcccgccg gcatacgcgt gcggagacgc cgttcttcac
     1381 cggcccgcgg gtgccggtcg agcagctcgt gaactcgcta ccttaacgaa gggggataca
     1441 tgacaacgca attcgacccg accgacccca cccgcttcga ggagatgtac cgcgacgacc
     1501 ggctgtcgca cggattgccc gccgccacgc cgtgggacat cggtggcccg cagccggtag
     1561 tccagcagtt ggtctcgttg ggcgcgatca aaggtgaggt gctcgacccg ggcaccggcc
     1621 cgggtcatca cgcgatctac tacgccacca agggctactc ggcgaccggc atcgatggct
     1681 cggagggtgc cctggagcgg gcccgggaga acgcccggaa ggccggggtg tcggtgaaat
     1741 tcgagctggc ggacgccacc aagctggatg gcctggacaa acggttcgac accgtcgtcg
     1801 actgcgcctt ctaccacacc ttcagcaccg agcccgaaat gcagaagtcc tatgtgcagg
     1861 cgctgcgccg ggccaccaag ccgggagcgc ggctgtacat gttcgagttc ggcgcgcacg
     1921 acgtcaacgg cttcacaatg ctgcggtcgc tgtcggaggc cgactttcgc gacgtgctgc
     1981 cgctcggcgg gtgggagatc acctacctgg gcccgacgac ctatcaggtg aacatgagcg
     2041 tcgagacgat cgagatgatg gccgcgcgca accctgacat ggccgacgag gccgagcgtc
     2101 tgctggaacg gttccgggcg atggagccgt ggctggtcaa cggccgggta catgccccgt
     2161 tctgggaagt tcacgccact cgggtcgact gacgccccaa ccgtcacttg cgtctccagc
     2221 ggacctgagt gggattttgc ccacctcgca gcgactgttg gcggcgcgat ccacgttgtc
     2281 gctcggaggc gggcattttg gcacaccgcc acttccagtg acggctggga cttctgagac
     2341 caacggcgcg cgacgagccg gctaagcctc cgcgggcacc aggcgcagcg aaaccgagtt
     2401 gatgcagtag cgctgatcgg tgggcgtcgg atagccctca ccggcgaaga cgtggcccag
     2461 gtggctgtgg cagttcgcgc agagcacctc ggtgcgcgtc gccccgaacg aatgatcggg
     2521 acgcaggatc accgcgtcgg agttcgcggg gtcgaagaac gacggccagc cgcagtgtga
     2581 atcgaatttc tctgtgctgc ggaacaattc agccccgcac gcccgacact ggtagacgcc
     2641 ctgggtcttg gtgtcggtgt actcgccggt gaacggtcgc tcggtaccgg cgcggcggag
     2701 cacgtcgaac tcctgggggc tgagtttctt acgccactcg tcgtcagaca gctgcacctt
     2761 cgggcgcgac aagtcgggtg acagatcggg aggagtcatg cgtccacgct aaccgcggag
     2821 ccaggtcgga tccaagccgc tgtccagccg gttctcggcc ttggcgtcca gatagcggaa
     2881 gtagagcacc gtgaacacca cgatcagcag cagcgaccac ccgtaggtga tcttcaggta
     2941 ctgcagcgcg cggccatacc tcatccagtt gaacagcagc cagttgtcca gcgtcatgaa
     3001 gccgtacacc cccagccagg cgggccaatt gcggatcagc gaattgggca gcacgaccgt
     3061 catcaggaac ggcaacagca tcatcgagta gtagccctgc gccagcggca gcgccagcca
     3121 cgaccacagc agcagcacgc ccgacgagct ggtgaaccag aacagcgggt cgcgggtgcg
     3181 gtagtagcgg tacagcagcc acatcgcgcc gatcgccaac accgtgaaca ggagtcgcag
     3241 gaacacgatc agccaggtcg gcagcccgaa gtacacaccg ttgccctcga ccgagctgtt
     3301 ga
//
LOCUS       DASSGQ010000082        12020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_400995,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000082 DASSGQ010000000
VERSION     DASSGQ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12020
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400995"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VFI55_03695"
     CDS             complement(<1..744)
                     /locus_tag="VFI55_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HET7073171.1"
                     /translation="MVTITKLSPADAIAEIGASPPGPRIGAFFDLDGTLIDGFTASVH
                     VGDRIRRRQAGIGELLGVLAGALRYRFGRLEFEQLIIRAAGYLRGETLAELDELGERL
                     FVERVASRLYSHMHEVVQAHQARGHTVVLSSSALSIQALPVARFLGIANVQCNHFELD
                     ERGCLTGGIVKPIVWGATKATAVERFCADNNVAVQRSYFYADGDEDAASMSLVGYPRP
                     VNPRSGLAAAAATHGWPVMCLASGRRGPVR"
     gene            complement(751..1671)
                     /locus_tag="VFI55_03700"
     CDS             complement(751..1671)
                     /locus_tag="VFI55_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7073172.1"
                     /translation="MTSTSPEFGSLRSDDDQWDIVSGVGYTALLVAGWRALHAAGPHP
                     LVRDDFAKVFIAASGDPYLAGALANPGTSEDETAFPRLYGVQTRFFDDFFTAAGDAGI
                     RQAVIVAAGLDSRAYRLDWADGTTVFEVDLPKVLEFKTNSLNEHGAAPKARRINVAAD
                     LRTDWATPLKAAGLDPQAPSAWSVEGVLPYLTDDAQSALFTRISELCAPGSRVAVGAL
                     GSRLDRERLAALETDHPGVSMSGDVDFSALTYEPKTDPADWLAAHGWAVEPVRNTLEL
                     QAGYGMTPAEVDVKIDGFMRSQYITAIRLG"
     gene            complement(1735..2166)
                     /gene="dtd"
                     /locus_tag="VFI55_03705"
     CDS             complement(1735..2166)
                     /gene="dtd"
                     /locus_tag="VFI55_03705"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HET7073173.1"
                     /translation="MRVLVQRVSSAAVSVDGQVVGAIRPDDQGLLAFVGVTHSDDPGK
                     ARRLAEKLWNLRILDDERSAADVNAPILVVSQFTLYADTAKGRRPSWNAAAPGAVAEP
                     LVAAFAEALRELGAHVQTGVFGANMQVALVNDGPVTVFLEL"
     gene            2218..2523
                     /locus_tag="VFI55_03710"
     CDS             2218..2523
                     /locus_tag="VFI55_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HET7073174.1"
                     /translation="MSVLVAFSVTPLGVGESVGEIVADAVRVVRDSGLPSKTDSMFTV
                     IEGDTWEQVMGVVQRAVEAVAARAPRVSTVIKVDWRTGVTDAMTQKVASVERYLSDG"
     gene            complement(2520..3014)
                     /locus_tag="VFI55_03715"
     CDS             complement(2520..3014)
                     /locus_tag="VFI55_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HET7073175.1"
                     /translation="MIAIEVVQADVTKLDVDAISNAANTQLLHGGGVAAAISRAGGPQ
                     VQRESTEKAPIGLGDAVETTAGDMPARYVIHAATMELGGPTSAEVIERATGATLRKAD
                     ELGCRSLALVAFGTGVGGFPLEDAARLMVDAVRQHRPRSLSKVVFAVHGDAAERAFRD
                     AVQG"
     gene            complement(3019..4398)
                     /locus_tag="VFI55_03720"
     CDS             complement(3019..4398)
                     /locus_tag="VFI55_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7073176.1"
                     /translation="MAGKSGAPRTRYAECGDVDIAYQVLGDGPDLVVFPGPFVPIGSI
                     DDEPSMYRFHRRLASFCRVIRFDHRGIGLSSPVNSMDELQPHFWAQDAIAVMDAVGCE
                     QATIFAPSVHAMNGLVLAADYPERVRGLVIVNGSARTLWAPDYPAGVQHRGANPFTTV
                     ALQPDAMEQGFDVLRIVAPTVADDDAFRAWWDLAGNRAGAPSMARAVSRAVSKGDVRE
                     ALSRITAPTLILHRVDSTFVPVGHGHYLAEHIAGSRFVELPGADVLYWVGDTARLLDE
                     VEEFITGAPGGFHSERVLATIVFTDIVRSTQRAALLGDGRWRDLLDNHDSIVRHELQR
                     FGGREVNTAGDGFVATFTSPSAALGCADEIVDAVRVLGIEVRVGIHAGEVEVRGDDVA
                     GMAVHIGARVAALAASGEVLVSSTVREIVAGSRRAFDARGERELKGVPGRWRLHALVR
                     EQDRSSARQ"
     gene            4447..5730
                     /locus_tag="VFI55_03725"
     CDS             4447..5730
                     /locus_tag="VFI55_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET7073177.1"
                     /translation="MAVSARAGIVVTGTEVLTGRVQDANGPWIADRLLELGVELAHIT
                     ICGDRPSDIDAQLRFMAEQGVDLIITSGGLGPTADDMTVEVVARFCGRELVLDSETEN
                     KIAAILKSFMARFDGVDFDAVRAANRKQAMIPAGAQVLDPVGTAPGVVVPGKPTVIVL
                     PGPPRELQPMWRSAIATPAAQQAIAGRTSYQQKTIRMFGLPESGLAETLRTAASIPGF
                     DALEITTCLRRGEIEMVTRYEPDAADAYALLTELLRDRHGQQIYSEDGSHVDDLVARL
                     LAGRRIATAESCTAGLVAARLTDRPGSSDYVLGGVVAYSNDAKTELLGVDAALIEAHG
                     AVSEPVAEAMAAGALRRFGADIAVATTGIAGPGGGTAEKPVGTVCFSVMLGPPGGRTI
                     TRTLRLPGNRSDVRDRSTTVAMHLLWRMLSGAESG"
     gene            complement(5732..6943)
                     /locus_tag="VFI55_03730"
     CDS             complement(5732..6943)
                     /locus_tag="VFI55_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7073178.1"
                     /translation="MLGWTMDAFDYFIVVFVYADIAKTFHISRAEVAFVTTATLVMRP
                     VGALLFGLWADRVGRRTPLMVDVIFYSVVGFLCAFAPNFTVLVILRMLYGLGMGGEWG
                     LGAALAMEKVPAERRGFFSGLLQEGYAFGYLLASLGSLVVMDWLGLSWRWLFGLSIVP
                     ALISLIIRYRVQESEVWEATQDQMKLTNTRLRDVLRDGAIVRRFIYLVALMTAFNWMS
                     HGTQDVYPTFLGSTTNQGAGLPSSTVKLIVVVYNIGAIIGGLFFGTQSQRFGRRYTVV
                     FCALLALPIVPLFAYSHSAAMLCLGSFLMQLCVQGAWGVIPAHLTEMSPDSIRGLYPG
                     VTYQLGNLLAAFNLPIQERLAESHGYPFALTVTIVPVLLAVALLTLVGKDATGIRFGT
                     AESAFVPTQVK"
     gene            7069..8136
                     /locus_tag="VFI55_03735"
     CDS             7069..8136
                     /locus_tag="VFI55_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7073179.1"
                     /translation="MRIIDADGHVAENPSLAIEAIKRWPQHVKPSTDGRLLLTLEGRN
                     YPEDQGPGAGCPPEHGITAAPGVNCRSAEGVLGDADRDHIDTMVLYPSLGLCVPSLEN
                     PDFAAGFARLYNQWIADYCAPSGGRLRGVAVTPVEHGQAAIDIMREAKDLGLVATLVP
                     PALKTRNLDHPDLDPFYAAAVELGMPLGIHGAPGIHLPKIGVDRFTNYIQVHCISFPF
                     DQMTAMTALVSGGVFERHPQLRVAFLEAGAGWVPFFIDRLHEHYEKRGDWVEHGWRRD
                     PHEYLAAGNIWVTCEPEEPILPGVIDVLGADFIMFASDYPHWDGDWPESTKHLRTRSD
                     ISEEAREKIGGRNAQRFYNLN"
     gene            8182..8583
                     /locus_tag="VFI55_03740"
     CDS             8182..8583
                     /locus_tag="VFI55_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET7073180.1"
                     /translation="MGAFLLRAAVTGFALWIVTLFVHGLSFVGGDSTLQRIGIIFVVG
                     VIFGLVNAFIKPVVQFLSIPLYILTLGLFHIVINALMLWITARITEHTTHWGLQIDHF
                     WWTAIWAAIVLSIVSWVLSLVVGDVGRRTRV"
     gene            complement(8588..9370)
                     /locus_tag="VFI55_03745"
     CDS             complement(8588..9370)
                     /locus_tag="VFI55_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET7073181.1"
                     /translation="MSIDYTLDAHIATITIDRPETRNSLDMAHFRDLARAWAAFRDDA
                     GAWVAVITGVGGDFCTGADLKKFIPELTGDLPKPDGWNKDDAIHAVLHRFPLYKPIIA
                     AVNGTCVAGGFEMLTSTDIRVAVPEARFAVMEPKRGLFAGGGSTVRLPRQLPYPLAME
                     LLLTADLVDAERALTMGLINRVVPAARLMDAAYDYAERVAANAPLAVRATKQSAVEGL
                     ALDLESAYENETRHSDRVFASEDAKEGPRAFAEKRPPRWQAR"
     gene            complement(9392..10360)
                     /locus_tag="VFI55_03750"
     CDS             complement(9392..10360)
                     /locus_tag="VFI55_03750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7073182.1"
                     /translation="MTRSARPIVVIGAGVSGLTSAISLAEAGWPVRVWAAAPPQQTTS
                     IVAGAVWAPPRPAERAASTLGWTEHSLRVFRELAEDPGTGVRMAPALIVGELTGAQAM
                     SSAAELIPDLRPADPADLPEGFGVGFHATMPMIDMPQYLAYLTRRLAAAGCEIEEHPV
                     RSLAEAAGVAPIVVNCAGLGAAALAGDDAMRPVFGQHVVLTNPGLRQLFLEINSGPEW
                     TCYFPHPQRIVCGGISIPGRWDTTPDPEVTDRILRRCRRVEPRLGRAEVIETITGLRP
                     DRPSVRVEAEPLGSSRAIHNYGHSSNGVTLSWGCAREVVRLANGDR"
     gene            complement(10374..10847)
                     /locus_tag="VFI55_03755"
     CDS             complement(10374..10847)
                     /locus_tag="VFI55_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073183.1"
                     /translation="MWSKAAPTAASFLVAGVMLVGWGATAGVAGADPEPAPSPSPAPK
                     TTMDHDGTYAVGTDITPGTYSSAGPVGNGTCYWKRIGNPDGNLIDNALSKKPQVVQID
                     PTDKAFKTTGCQPWQPTSDAPPPTVPGPAAGANLQNDLGVLNGLLAPNGQQVPQS"
     gene            11017..11358
                     /locus_tag="VFI55_03760"
     CDS             11017..11358
                     /locus_tag="VFI55_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073184.1"
                     /translation="MTDTADDVAERLDGTTPVPANETVSFGGENVPVWRPVTLYRTVT
                     DSQDRNVPRTNSPAAQPWNTPSTLDQATVLAEDLTVVYQDPQGGQWTLSDMIIELFKA
                     HLGLPTKAAGA"
     gene            11358..>12020
                     /locus_tag="VFI55_03765"
     CDS             11358..>12020
                     /locus_tag="VFI55_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HET7073185.1"
                     /translation="MANTWADVADRLDGIYSGAPSFAGNLPIILLCWQGTGSDMWNTI
                     PPQPASVATVVAQAFPEILHFQPVGNYPAATYPMGPSVADGYNEGIRLATQVYPNNLL
                     LPFGYSQGAICASHFLRDFIMPNNLLNRVVGGITFGNPCRSPGVANGNAWAGWPMPGE
                     IDGVVTGGIAGPDCLTAAQTPTWWLDFVNTTTSDKGASELYGNCPVGTDPWTAEAAPG
                     KVG"
BASE COUNT         2022 a         4144 c         3986 g         1868 t
ORIGIN      
        1 ccgcaccggc ccgcggcggc cggaggcgag gcacatcacc ggccagccgt gcgtcgcggc
       61 cgccgccgcg aggcccgagc gggggttcac cggccgcgga tacccgacga gtgacatcga
      121 tgccgcgtcc tcgtcgccgt cggcgtagaa gtaactgcgt tgcaccgcaa cgttgttgtc
      181 ggcgcagaac cgttcgacgg cggtcgcctt cgtcgctccc cacacgatcg gtttgacgat
      241 tccgccggtc aggcagccgc gctcgtcgag ttcgaaatgg ttgcactgca cattggcgat
      301 accgagaaag cgtgcgacgg gcagtgcctg gatggacagc gccgacgaac tcagcaccac
      361 agtgtgtccg cgggcctgat gggcctgcac aacttcatgc atgtgggagt agagccgcga
      421 ggcgacgcgt tccacaaaca gccgctcacc caactcgtcg agctcggcga gcgtttcgcc
      481 acgcagataa cccgcggccc ggatgatcag ctgctcgaac tccaggcggc cgaaccgata
      541 ccgcagcgcg cctgccaaaa caccgaggag ctcgccgatc ccggcctggc gacgccggat
      601 ccggtcaccg acatgcaccg acgcggtgaa gccgtcgatc aatgtcccgt cgaggtcgaa
      661 gaacgcaccg atcctgggtc ccggcgggct ggcgccgatc tccgcgatgg cgtccgcggg
      721 ggagagcttc gtgatcgtca ccatctcgcc tcaccccaat cggatcgcgg tgatgtactg
      781 cgagcgcatg aagccgtcga tcttgacgtc cacctcggcg ggcgtcatcc cgtagcccgc
      841 ctgcaactcc aaggtgttgc ggaccggttc gacggcccag ccgtgcgcgg ccagccaatc
      901 cgccgggtcg gtcttgggtt cataggtcag cgcggagaag tcgacgtccc ccgacatgct
      961 gaccccgggg tgatccgttt ccagggcggc gagccgttcg cgatccaggc gcgatcccag
     1021 cgcgcccacc gcaactcggc tgcccggggc acaaagttcg ctgatccggg tgaacagtgc
     1081 gctttgcgcg tcgtcggtca ggtagggcag cactccctcc accgaccagg cgctcggggc
     1141 ttgtggatcc aacccggccg ctttcaacgg cgtcgcccag tcggtgcgca ggtcggccgc
     1201 gacgttgatc ctgcgcgctt tgggggcggc gccgtgttcg ttgagcgagt tggtcttgaa
     1261 ttccagcact ttcggcagat cgacctcgaa aaccgttgtg ccgtcggccc agtcaaggcg
     1321 atacgcgcgg gaatccagcc ccgcggcgac gatcaccgcc tgccggatgc ccgcgtcgcc
     1381 cgcggcggtg aagaagtcgt cgaagaatcg ggtttgcacg ccgtacaggc ggggaaaggc
     1441 cgtctcgtcc tcagacgttc ccggattggc aagagccccg gccaaatacg ggtcgcctga
     1501 agcggcgatg aaaacctttg caaagtcgtc acggaccagc gggtgcgggc ccgcggcgtg
     1561 caacgcccgc catcccgcca ccagcagggc ggtgtacccc acgccgctga cgatgtccca
     1621 ctggtcgtcg tccgaacgaa gcgagccgaa ttcaggtgag gtggatgtca tgggccctcc
     1681 ccatctacgc ggcaaaacat agccgacgta accaacgtac ccgcggcggc gccgtcacag
     1741 ctcgagaaac accgtgactg ggccgtcgtt caccaacgcg acctgcatgt tcgcgccgaa
     1801 cacgccggtc tgtacgtggg cgcccagctc ccgcaacgcc tccgcgaacg ccgctaccag
     1861 cggctcggcg accgcaccgg gagctgcggc gttccaggac ggccggcgcc ccttcgcggt
     1921 gtcggcgtac agggtgaact ggctgacgac caggatcggc gcgttgacgt cggccgcgga
     1981 ccgttcgtcg tcgagaatcc gcagattcca cagcttttcg gccaaccgcc gcgccttgcc
     2041 gggatcgtcg ctgtgggtca caccgacgaa cgcgagcagt ccctggtcgt ccggccggat
     2101 cgcgcccacc acctggccgt cgacggatac cgcagccgat gagacccgtt gcaccagaac
     2161 ccgcatgggc ctcgatgctg ccaggtcggc ccagaaaagg ttgggtacgc tcgtcgggtg
     2221 tccgtgctcg tcgcgttttc cgtcaccccg ctgggtgtcg gtgagagcgt cggcgagatc
     2281 gtcgccgacg cggttcgcgt ggttcgcgat tccgggctgc ccagcaagac ggattcgatg
     2341 ttcaccgtga tcgaaggcga tacgtgggaa caggtgatgg gcgtcgtgca gcgcgcggtc
     2401 gaggccgtcg cggctcgcgc gccccgcgtc agcacggtga tcaaggtgga ctggcgcacg
     2461 ggagtaacgg acgcgatgac gcagaaggtc gcctccgtcg agcgttatct gtccgacggc
     2521 tagccctgca cggcgtcccg gaacgcccgc tcggcggcgt cgccgtggac cgcgaacacg
     2581 accttggaaa gcgagcgcgg ccgatgttgc cggacggcgt cgaccatcag ccgcgccgcg
     2641 tcttccagcg ggaagccgcc gacgccggtg ccgaacgcca ccagcgccag cgaccggcaa
     2701 ccgagctcgt cggctttgcg cagggtagcg ccggtcgccc gctcgatgac ctcggccgag
     2761 gtcgggccgc cgagctccat ggtcgccgcg tggatcacgt agcgcgccgg catgtcgccc
     2821 gccgtggtct cgaccgcgtc cccgagcccg atcggcgctt tttcggtcga ctcacgctgc
     2881 acctgtggcc cgccggcgcg ggagatcgcc gcggcgacac cgccgccgtg cagcagttgg
     2941 gtattggctg cgttgctgat cgcgtcgacg tcgagcttcg tcacgtcggc ctgcacgacc
     3001 tctattgcga tcatcgattc attgtctcgc agacgatcgg tcctgctcgc gcaccagcgc
     3061 gtgcaaccgc caccggcccg gcacgccctt gagttcacgc tcgccgcgcg cgtcgaaggc
     3121 gcgccgcgag cctgcgacga tctcacgcac agtcgaggac accagcacct cgcccgaggc
     3181 ggccagcgcg gccacccgcg cgccgatgtg cacggccatg cccgcgacgt catcgccgcg
     3241 cacctcgacc tcacccgcgt ggatacccac ccgcacctcg atgccgagca cccgcaccgc
     3301 gtccacgatc tcgtcggcgc agccaagcgc ggcgctgggg ctggtgaacg tcgcgacgaa
     3361 cccgtcaccg gcggtgttca cctcgcgccc gccgaaccgc tgcagctcgt ggcgcacgat
     3421 gctgtcgtgg ttgtccaaca ggtcgcgcca ccggccgtcg ccgagcaggg cggcgcgctg
     3481 cgtggaccgg acgatgtcgg tgaacacgat cgtggccagc acgcgctcgc tgtggaaccc
     3541 gcccggcgcg ccggtgatga actcctcgac ttcgtcgagc aaccgcgcgg tgtcacccac
     3601 ccagtacagg acgtcggcgc ccggtagctc gacgaagcgc gatcccgcga tgtgctcggc
     3661 gaggtagtgc ccgtgcccga cgggaacgaa ggtcgaatcg acccgatgca ggatcagcgt
     3721 cggcgcggtg atgcgcgaga gagcttcgcg cacatcgcct ttggaaaccg ccctcgacac
     3781 cgcgcgggcc atgctcggcg cacccgcgcg gttgccggcc agatcccacc aggcccggaa
     3841 cgcgtcgtca tcggccaccg tcggcgccac gatgcgcaac acgtcgaaac cctgctccat
     3901 ggcgtcgggt tggagcgcga cggtggtgaa cggattggcg ccccggtgct ggacgccggc
     3961 cgggtagtcg ggcgcccaca gcgtgcgcgc ggagccgttg acgatcacca ggccgcgcac
     4021 ccgctcggga taatcggcgg caagcaccag cccgttcatt gcgtgcacac tcggcgcgaa
     4081 gatcgtggcc tgctcgcatc cgaccgcatc catcaccgca atcgcgtctt gcgcccaaaa
     4141 gtggggttgc agctcgtcca tcgagttcac cggagacgac agaccgatcc cgcggtggtc
     4201 gaaccggatc acccggcaaa acgacgccag gcgccgatgg aagcggtaca tcgacggctc
     4261 atcgtcgatg gagccgatcg ggacgaaggg ccccggaaac acgacaaggt caggtccgtc
     4321 accgagcacc tggtaggcga tgtccacatc gccgcattcg gcgtagcgag tccgcggcgc
     4381 cccagacttc ccagccacgg ccccaggcta gttcaggcca ccgtcaagaa tcgcgcaagt
     4441 aggctcgtgg cggtgagcgc acgcgcgggc atcgtcgtca ccggaactga agtcctcacc
     4501 ggccgcgtcc aagacgccaa cggcccctgg atcgccgatc gcctgctgga gttgggcgtg
     4561 gagctggccc acatcaccat ctgcggcgac cgcccctccg acatcgatgc gcagctgcga
     4621 ttcatggccg agcagggcgt ggacctgatc atcaccagcg gcggcctggg ccccacggcc
     4681 gacgacatga cggtcgaggt ggtggcccgt ttctgcggcc gcgagctggt gttggacagc
     4741 gaaaccgaaa acaagatcgc cgccatcctc aaatctttca tggcacgctt cgacggcgtc
     4801 gacttcgacg cggtgcgcgc cgccaaccgc aagcaggcca tgattcccgc gggtgcgcag
     4861 gtgctcgatc cggtgggcac ggcgcccggc gtcgtggtcc ccggcaaacc gacggtgatc
     4921 gtgcttccgg gcccgccacg cgagctgcag ccgatgtggc gcagcgctat cgcgaccccc
     4981 gctgcgcagc aggcgatcgc cggccgaacg tcctaccagc agaagaccat tcggatgttc
     5041 gggctgcccg agtcgggcct ggccgaaacg ctgcgcacgg cggcgtccat ccccggcttc
     5101 gacgcgctgg agatcaccac ctgcctgcgt cgtggggaga tcgaaatggt cacccgctac
     5161 gaacccgacg ccgcggacgc ctacgcgcta ctgaccgagt tgctgcgtga ccggcacggg
     5221 cagcagatct actccgaaga cggctcgcac gtcgacgatc tggtcgcgcg gttgctggcc
     5281 gggcgccgga tcgcgacggc ggagtcctgt accgcggggc tggtggcggc gcggctgacc
     5341 gaccggcccg gttcgtccga ctacgtgctg ggcggcgtgg tggcctactc caacgacgcg
     5401 aaaacggagc tgctcggcgt cgatgccgcc ctgatcgagg cgcacggtgc ggtgtccgag
     5461 ccggtggccg aggcgatggc cgcgggcgcg ctgcggcgct tcggcgccga catcgccgtc
     5521 gcaaccaccg gaatcgccgg ccccggcggg ggaaccgccg aaaagccggt gggcacagtc
     5581 tgtttcagcg tgatgctcgg ccctcccggc ggccgcacga tcacccgcac cctgcggctc
     5641 cccgggaacc ggtccgacgt ccgggatcgg tcgacgacgg tggcgatgca cctgttgtgg
     5701 cgcatgctga gcggcgccga gtcgggttag gtcacttcac ttgggttggc acaaaggcgc
     5761 tttccgcggt gccgaatcgt attccggtgg cgtccttgcc gaccagcgtc agcagcgcga
     5821 cggcgagcaa caccggcacg atcgtgacgg tcaacgcgaa cgggtagccg tgcgactcgg
     5881 cgaggcgttc ctggatcggc aggttgaacg cggcgagcag attgccgagc tggtaggtga
     5941 cgccggggta gaggccacgg atggagtccg gcgacatctc ggtcaagtgc gcggggatga
     6001 caccccaggc accctgcacg cacagctgca tcagaaacga gcccaggcac aacatcgccg
     6061 cggagtgcga gtaggcgaag agtggcacga tcggcagcgc gagcagggcg cagaaaacca
     6121 cggtgtagcg acggccgaac cgctgtgact gcgtgccgaa gaacagtccg ccgatgatgg
     6181 cgccgatgtt gtagaccacc acgatcaact tgacggtcga gctgggcagc ccggcgccct
     6241 ggttggtggt cgaccccagg aaggtggggt agacgtcctg ggtgccgtgg ctcatccagt
     6301 tgaacgcggt catcaacgcc accaggtaga tgaaccggcg gacgatggcg ccgtcgcgca
     6361 gcacgtcgcg gagtctggtg ttggtcagtt tcatctggtc ctgggtggct tcccacacct
     6421 cggactcctg tacccggtac cggatgatca ggctgatcag ggccgggacg atggacaaac
     6481 cgaacaacca gcgccacgac aaccccagcc aatccatcac gaccagcgat cccaggcttg
     6541 ccagcaggta accgaatgcg tagccctcct gcagcagccc ggagaagaag ccgcgccgct
     6601 cggcgggaac cttctccatg gcgagcgcgg cgcccagtcc ccattcgccg cccatgccaa
     6661 ggccgtacag catccgcagg atcaccagca cggtgaagtt gggcgcgaac gcgcacaaga
     6721 agccgaccac ggagtagaag atcacatcca ccatcaacgg cgtccgtcga ccgacccggt
     6781 cggcccacag cccgaacaac aacgcgccca ccgggcgcat caccagggtg gcggtcgtga
     6841 cgaacgcgac ctcggccctg ctgatgtgga aggtctttgc gatgtcggcg tagacgaaca
     6901 ccacgatgaa gtaatcgaag gcgtccatcg tccagcccaa catcgccgca atgaacgagt
     6961 tccgctgatc ggcgttgatc ttctgggtct ttggagttgt cacgtgagaa tcctggccca
     7021 cccgcaagcc gtttgaccag acaacccgcg agagtaagtt ccggatgcat gcgaatcatc
     7081 gacgccgacg gacacgtcgc cgaaaaccct tcgctggcga tcgaggcgat caaacgctgg
     7141 ccgcagcacg tcaaacccag caccgacggg cggttgctat tgacgctgga aggccgcaac
     7201 tacccggaag accagggccc cggcgccggt tgtccgcccg agcacgggat caccgcggcg
     7261 cccggcgtca actgccggtc agccgagggc gtgctgggcg acgccgaccg cgaccacatc
     7321 gacacgatgg tgctgtatcc cagcctcggg ctgtgcgtgc ccagcctcga aaacccggat
     7381 ttcgcagcgg gattcgcgcg gctctacaac cagtggatcg cggattactg cgccccgtca
     7441 ggcgggcggc tgcgcggcgt cgcagtgacc cccgtcgagc acggccaggc ggccatcgac
     7501 atcatgcgcg aggccaaaga cctcgggctg gtggcgacgc tcgtcccgcc cgcactcaag
     7561 acccgcaacc tcgaccaccc cgacctcgat ccgttctacg cggccgccgt cgaactgggg
     7621 atgccgctgg gcattcacgg cgcgcccggc atccatctgc ccaagatcgg cgtggaccgc
     7681 ttcaccaact acatccaggt gcactgcatc agctttccgt tcgaccagat gacggcgatg
     7741 accgcgctcg tctccggcgg tgtcttcgaa cgccatccgc agctgcgggt cgccttcctc
     7801 gaggcgggcg cgggctgggt gccgttcttc atcgatcgcc tgcacgagca ctacgagaag
     7861 cggggagatt gggtcgagca cggctggcgc cgcgatccgc acgagtatct ggccgcgggc
     7921 aatatctggg tgacctgcga gccggaagag ccgatcctgc ccggtgtgat cgacgtgctc
     7981 ggcgccgact tcatcatgtt cgccagcgac tacccacatt gggacggcga ttggccggag
     8041 agcaccaaac accttcgaac gcgatcggac atcagcgagg aggctcgcga gaagatcggc
     8101 ggacgcaacg cgcagcgttt ctacaacctg aactgaggcc tgctgacgtc gagggggccc
     8161 gcggtggcag gatcttcgga catgggtgcc tttctgctgc gtgccgcggt gaccggattt
     8221 gccttgtgga tcgtcaccct tttcgtccac gggctgagct tcgtcggcgg cgacagcacg
     8281 ctgcagcgga tcggcatcat cttcgtcgtc ggcgtgattt tcggtctggt caatgcgttt
     8341 atcaagcccg tcgtccagtt cctgtcgatc ccgctgtaca tcttgaccct tggcctgttc
     8401 cacatcgtca tcaacgcgtt gatgttgtgg atcaccgccc ggatcaccga acacaccacg
     8461 cactgggggc tgcagatcga ccatttctgg tggacggcga tctgggcggc catcgtgttg
     8521 tccatcgtga gctgggtgct gtcgctggtg gtgggtgacg tcggtcgccg cactcgggtc
     8581 tgagggctca gcgggcctgc caccgcggcg gtcgcttctc ggcgaaggcg cgcggtccct
     8641 ccttcgcgtc ctcgctggcg aaaacgcggt cgctgtgccg ggtttcgttc tcgtaggccg
     8701 actcgaggtc cagagccaaa ccttcgacgg ctgactgctt ggtggcgcgg accgcgagcg
     8761 gtgcgttggc cgcgacgcgc tcggcgtagt catacgcggc gtccatcagc cgggccgccg
     8821 ggaccacccg gttgatcagc cccatcgtca gggctcgctc cgcgtccacc aggtcggcgg
     8881 tcagcaacag ctccatcgcc aacgggtagg gcagctggcg cggcagccgc acggtgcttc
     8941 caccgccggc gaagagaccg cgcttgggct ccattaccgc gaatcgcgcc tcgggcacgg
     9001 caacgcgaat atcggtggag gtcaacatct cgaagccgcc cgcgacgcag gtaccgttga
     9061 cggccgcgat gatcggcttg tacagcggga accgatgcag gactgcgtgg atggcgtcgt
     9121 ccttgttcca tccgtcgggc ttgggtaggt caccggtgag ttccggaatg aatttcttga
     9181 ggtcggctcc cgtacagaag tccccgccga cgccggtgat caccgcgacc cacgcgccgg
     9241 cgtcgtcgcg gaaggccgcc caggcgcggg ccagatcgcg gaagtgtgcc atgtccagcg
     9301 agttgcgtgt ctcggggcgg tcgatcgtga tggtggcgat gtgcgcgtcc agcgtgtagt
     9361 cgatgctcac aagcttcgac gttagacacc gctaacggtc gccattcgcc aggcgcacca
     9421 cctcgcgcgc gcagccccac gacagcgtca cgccgttgct gctgtggccg tagttgtgga
     9481 tggcgcggct gctgccgagc ggctcggctt ccacccgcac cgacgggcgg tcgggacgca
     9541 gcccggtgat cgtctcgatc acctcggccc ggccaagccg cggctcgacc cgccggcacc
     9601 gtcgcaggat ccggtcggtc acctcgggat ccggggtggt gtcccagcgc ccggggatgc
     9661 tgatgccgcc gcacacgatg cgctgcgggt gcgggaagta gcaggtccat tccgggccgc
     9721 tgttgatttc cagaaacagt tgccgcagac cgggattggt cagcacgacg tgctggccga
     9781 acaccggccg catcgcgtcg tcgcccgcca gcgctgccgc gcccaaaccc gcacagttga
     9841 cgacgatggg tgcgacaccg gcggcctcgg ccagcgaccg cacgggatgc tcctcgattt
     9901 cgcacccggc ggcggcgagc cgccgggtca ggtaggcaag gtattgcggc atgtcgatca
     9961 tcggcatggt ggcgtggaac ccgacgccga agccctccgg gaggtcggcc gggtcggccg
    10021 gacgcaggtc ggggatcagc tcggccgccg acgacatcgc ctgggccccg gtcaattcgc
    10081 cgacgatcag cgccggcgcc atccgcaccc cggtgccggg gtcctcggcc agttcgcgaa
    10141 acacccgcag ggagtgctcg gtccaaccca gcgtcgaggc ggcacgttcg gccggtcgcg
    10201 ggggcgccca taccgcgccc gccacgatcg aggtcgtctg ctgcggcggt gcggccgccc
    10261 acacgcgcac cggccacccc gcttcggcca agctaatcgc cgacgtcaac ccactcacgc
    10321 cggcaccgat cacgacgatt ggtcgcgcgc tacgtgtcac ggcggcggac ccgtcaggac
    10381 tgcggcacct gctgtccgtt cggggccagc agcccgttca ggacgccgag atcgttttgc
    10441 agattggcgc ctgcggccgg tccgggcaca gttggcggcg gggcgtcact cgtcggctgc
    10501 cagggctggc agccggtcgt cttgaacgcc ttgtcggtcg ggtcgatctg cacgacctgc
    10561 ggcttcttgc tcaaggcgtt gtcgatgagg ttgccgtcag ggttgccgat ccgcttccag
    10621 tagcacgtgc cgttgccgac cggtccggcc gaactgtagg tgccgggcgt gatgtcggtc
    10681 cccaccgcgt aggtgccgtc gtggtccatc gtcgtcttgg gcgcgggtga cggggacggc
    10741 gccggctccg gatcggctcc ggcaacgccg gcagtcgcac cccagccgac aagcatcacg
    10801 cccgcgacga gaaagcttgc cgctgtcggt gctgcctttg accacataga acgcaagcct
    10861 accggggtgg gcgacccatt tcgaaccgcg agaccggcgt cagcatcggc gtctcatcag
    10921 gcccgaaatc aagtagcgca ctacgcgtgc cgtcatcgcg cgtcgttgaa accgtgtgaa
    10981 gacaccccgc cgcggcccgc tgaccaagga gcgcacatga ccgacaccgc agacgacgtc
    11041 gccgaacgcc tcgacggcac cacccccgtc ccggccaacg aaacagtctc attcggcggt
    11101 gagaacgtgc ccgtgtggcg accggtcacc ctgtaccgga ctgtcaccga cagccaggac
    11161 cgtaacgtgc cgcgcaccaa ctcgccggcg gcccagccgt ggaacacccc gtcaacactc
    11221 gatcaggcca ccgtgctcgc cgaagacctg accgtcgtct accaagaccc gcagggcggc
    11281 cagtggaccc tttccgacat gatcatcgaa ctgttcaagg cgcatttggg gctgccgacg
    11341 aaagctgcgg gcgcgtaatg gcgaacacct gggccgacgt cgccgaccgc ctggacggca
    11401 tttacagcgg cgcccccagc tttgccggca acctgccgat aatcctgctg tgctggcagg
    11461 gcaccggttc agacatgtgg aacaccatac cgccgcaacc cgcatcggtg gcaaccgttg
    11521 tggcgcaagc ctttccggag atacttcatt tccagccggt gggaaattac ccggccgcaa
    11581 cctatcccat gggcccgagc gtcgccgacg gctacaacga aggcatccgt ctggcgacgc
    11641 aggtctaccc gaacaacctg ctgctcccgt tcggctacag ccaaggggcg atctgcgcga
    11701 gtcactttct gcgcgacttc atcatgccca acaacctgct gaaccgggtc gtgggtggca
    11761 tcacgttcgg aaacccctgc aggtcaccgg gagtcgcgaa cgggaacgcg tgggccggtt
    11821 ggcccatgcc gggagagatc gacggcgttg tgaccggcgg catcgccggg ccggactgcc
    11881 ttaccgcggc gcaaaccccg acctggtggc tggacttcgt caacaccact accagcgaca
    11941 aaggtgcgag cgagttatac ggcaactgcc ccgtcgggac tgatccgtgg acggcagaag
    12001 cggcaccggg gaaggtgggc
//
LOCUS       DASSGQ010000083        10059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1008698,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000083 DASSGQ010000000
VERSION     DASSGQ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10059
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008698"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VFI55_03770"
     CDS             <1..287
                     /locus_tag="VFI55_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073186.1"
                     /translation="GRVVVASYLLADGLFQERLHACGADLVGRPLGTHPGLARLIANR
                     FRRAVPEPLPSTMRHAARRPSLRRAQGPHALPSFKARAAVSPWRADARDA"
     gene            319..771
                     /locus_tag="VFI55_03775"
     CDS             319..771
                     /locus_tag="VFI55_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073187.1"
                     /translation="MFRREDRSRKLLDPVAKMLRLPFGTSEFIDRIVTGGVNQVGRRT
                     LYMLITAWDAAGGGPFAASTIAGTGIAKAAEVVENTFVGPFFGPLLKRLGADKAAVRA
                     SLCASQLVGLGIMRYGLRSEPLHSMSVDMVVDAIGPTMQRYLVGDITR"
     gene            complement(796..1152)
                     /gene="nirD"
                     /locus_tag="VFI55_03780"
     CDS             complement(796..1152)
                     /gene="nirD"
                     /locus_tag="VFI55_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HET7073188.1"
                     /translation="MTLLNDIQVWTTACAYDHLIPGRGVGVLLDDGTQAALFRLDDGS
                     VHAVGNVDPFSGAAVMSRGIVGDRGGRATVQSPLLKQAFALEDGSCLDDPRASVPVYQ
                     VRVTPEGQIQVGRIAS"
     gene            complement(1211..3775)
                     /gene="nirB"
                     /locus_tag="VFI55_03785"
     CDS             complement(1211..3775)
                     /gene="nirB"
                     /locus_tag="VFI55_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HET7073189.1"
                     /translation="MAPSETARAHCAPRHVVVVGHGMVGHRFVEALRARDTEGVWCIT
                     VLAEEADAAYDRVGLTSYTDSWDRGLLALPGNHYAGDERVRLVLNTRVTEIDRATKSV
                     VTADGQRHQFDALVLATGSYAFVPPVPGHDLPACHVYRTLDDLDAIRADAQRTMDGGR
                     ATAGVVIGGGLLGLEAANALRQFGLQTHVVEMMPRLMAQQIDEAGGALLARMVSDLGI
                     AVHVGTGTESIESVAASDGPTSLRVRLSDGELIDAGVVIFAAGIRPRDELAVAAGLTR
                     AERGGVLTDLSCRTSDPDIYAVGEVAAIGGRCYGLVGPGYTSAEVVADRLLDGTAEFP
                     EADLSTKLKLLGVDVASFGDAMGATENCLEVAINDAVNRTYAKLVLSDDAKTLLGGVL
                     VGDASSYGVLRPMVGSELPGDPVALIGPAQSDGVAALGIGSLPDSAQICSCNNVTKGD
                     LKCAIAGGCADVPALKSCTAAGTSCGSCVPLLKQLLEAEGVEQSKALCEHFSQSRAEL
                     FEIISATEIRTFSGLLERFGRGKGCDICKPVVASILASTGSDHILDGEQASLQDSNDH
                     FLANIQKNGSYSVVPRVPGGDIKPEHLILIGQIAQDFGLYTKITGGQRIDLFGARVDQ
                     LPQIWKRLVDGGMESGHAYGKALRTVKSCVGSDWCRYGQQDSVQLAIDLELRYRGLRA
                     PHKIKLGVSGCARECAEARSKDVGVIATEKGWNLYVGGNGGMTPKHAQLLVSDLDTET
                     LVRYVDRFLMYYIRTAERLQRTAPWVESLDGGLDHVREVVCEDSLGLAAEFEAAMERH
                     VQNYQCEWKGVLEDPDKLSRFVSFVNAPDAVDSTVTFTERAGRKVPVSIGMPRVRP"
     gene            4009..4458
                     /locus_tag="VFI55_03790"
     CDS             4009..4458
                     /locus_tag="VFI55_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7073190.1"
                     /translation="MTNLAVWSRSARDTDRWLRDFFGPAAAADWYKPLNSGFSPAAEI
                     VKDGDDAVVRLELPGVDVEKDVNVEVDKGRLVVHGERRDERTEEENGRTLREIRYGSF
                     RRSFKLPAHVTSEAVTASYDDGVLTVRVTGAHQDPAQPQAQRIEITK"
     gene            complement(4576..5334)
                     /locus_tag="VFI55_03795"
     CDS             complement(4576..5334)
                     /locus_tag="VFI55_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HET7073191.1"
                     /translation="MAGHTITERARALLELHQPGNPVVLPTVWDAWSARLATDAGFVA
                     LTVGSHPLADSIGKPDGEGMSFDDVLARVAQITAAVDVPVSADIESGYGQPAGRLIEG
                     LLSVGAVGLNIEDTVHSEGKRLRSAGEHAQLVGALRSAADAAGVHVVINARTDVFLRQ
                     DGEQSDRVERAVARLTEAAAAGADVLYPVGRHDPETLRRLATELPLPINAIALPELDD
                     PASFGPLGVGRISFGPFLQAALAVRARELLARWA"
     gene            5495..5752
                     /locus_tag="VFI55_03800"
     CDS             5495..5752
                     /locus_tag="VFI55_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073192.1"
                     /translation="MGGLRKCVHSLKARYGDSPAMRRIAIDADRILSDVELLETDLSE
                     LDLARATVMQSGEKIAIPDTQYDSDFWRDVDDEGVGGHARS"
     gene            5832..6653
                     /locus_tag="VFI55_03805"
     CDS             5832..6653
                     /locus_tag="VFI55_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073193.1"
                     /translation="MSAPTANRPASGVFSPGRAEIAQRTLRTDRWWQAPLLTNIGLLA
                     FIVYATTRAFWGSDYWVDQYHYLTPFYSPCVSASCEPGASHLGVWFGHFPWWIPLGSL
                     VLPFLLGFRLTCYYYRKAYYRSVWQSPTGCAVPEPRVHYTGETRLPLIIQNSHRYFFY
                     VALVVSLINTYDAIMAFHSPSGFGFGLGNVILVTNVLLLWAYTLSCHSCRHVTGGRLK
                     HFSKHPVRYWIWTQVSKINTRHMLFAWITLGTLSLTDFYIMLVASGTIADLRFVG"
     gene            6696..8615
                     /locus_tag="VFI55_03810"
     CDS             6696..8615
                     /locus_tag="VFI55_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HET7073194.1"
                     /translation="MVEVERRSYDVVVIGAGGAGLRAVIEARERGLKVAVVCKSLFGK
                     AHTVMAEGGCAASMGNTNPKDNWKTHFGDTMRGGKFLNNWRMAELHAKEAPDRVWELE
                     TYGALFDRTKDGKISQRNFGGHTYPRLAHVGDRTGLELIRTMQQKIVSLQQEDFAELG
                     DYEARIKVFAECTITELLKDGVRIAGAFGYWRESGRFVVFEAPAVVLATGGIGKSFKV
                     TSNSWEYTGDGHALALRAGASLINMEFVQFHPTGMVWPPSVKGILVTEGVRGDGGVLK
                     NSDGKRFMFDYIPPVFKGQYAETEQEADQWLKDNDSARRTPDLLPRDEVARAINSEVK
                     AGRGTPHGGVYLDIASRLTPAEIKRRLPSMYHQFMELAEVDITKQPMEVGPTCHYVMG
                     GVEVEPDTGAATVAGLFAAGECSGGMHGSNRLGGNSLSDLLVFGRRAGLGAADYVRAL
                     SSRPAVDQDAIDAAAKRALDPFEGPSNGAAAAENPYTLHLELQQSMNDLVGIIRNADE
                     ISQALDRLDKLRERFKNIQVEGHRQYNPGWNLAIDLRNMLLVSECVAKAALERTESRG
                     GHTRDDHPSMDSSWRKLLLVCRAAGGDPVIPDIAVTREDQVPMRSDLLELFEISELEK
                     YYTGAELAEHPGRRG"
     gene            8615..9361
                     /locus_tag="VFI55_03815"
     CDS             8615..9361
                     /locus_tag="VFI55_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HET7073195.1"
                     /translation="MTYNATMRVWRGDEAGGALEDFKVEVNEGEVVLDIIHRLQQTQT
                     PDLAVRWNCKAGKCGSCSAEVNGQPRLMCMTRMSTFAEDEVVTVTPLRTFPVIRDLVT
                     DVSFNYEKAREIPSFAPPKELQPGEYRMAQVDVARSQEFRKCIECFLCQNVCHVVRDH
                     EENKKAFAGPRFLMRVAELEMHPLDTRDRRNQAQEEFGLGFCNITKCCTEVCPEHIKI
                     TDNALIPMKERVVDRKYDPVVWLGNKLFRR"
     gene            complement(9433..>10059)
                     /locus_tag="VFI55_03820"
     CDS             complement(9433..>10059)
                     /locus_tag="VFI55_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PGRS family protein"
                     /protein_id="HET7073196.1"
                     /translation="GGAGGAGTTVGGAGGPGGASGLLFGAGGVGGAGGQGVTTGGAGG
                     VGGDAVGLFGSGGLGGVGGPSVGNGGVGGVGGNSAMVVGSGGVGGTGGPSSNGVGGAG
                     GAGGDGRGLIGSGGVGGTGGSGATTSGAGGVGGRAGVVGNGGNGGEGGVNFFLAGGPG
                     AGGNGGNAVLIGDGGNGGNPGSNAGGFLALAGAGGAAGLLFGLPGFNG"
BASE COUNT         1762 a         3430 c         3256 g         1611 t
ORIGIN      
        1 cgggtcgcgt cgtggtcgcc tcctatctgc tggccgacgg cctgtttcag gagcgtctgc
       61 atgcctgcgg cgccgacctg gtcggtcggc cgttgggcac ccatcccgga ctggcgcggc
      121 tgatcgcgaa ccgattccgc agggcagtgc ccgagccgct gccatcgacg atgcggcacg
      181 cagcccgtcg acccagcctg cggcgagccc aaggacccca cgcgctgccc agcttcaagg
      241 cgcgcgctgc cgtgtcgccg tggcgcgccg atgcccgcga cgcttgatct ttgatggcgc
      301 gcacgcaatc ctgacatcat gttccgtcgc gaagatcgat cgcggaagct ccttgatccg
      361 gttgcaaaga tgctgcggtt gcccttcggc acatcggagt tcatcgatcg catcgtcacc
      421 ggaggggtca atcaggtggg ccgccggacg ttgtacatgc tgatcaccgc gtgggatgcg
      481 gccggcggtg gcccgtttgc ggccagcacg atcgcgggca cggggatcgc caaggccgcc
      541 gaagtcgtgg agaacacgtt cgtcggcccg ttcttcggcc cgctgttgaa aaggctcgga
      601 gccgataagg cggccgtgcg ggcgtcgttg tgcgcctctc agctcgtcgg cctgggcatc
      661 atgcgctacg ggctacgctc cgagccgctg cactcgatgt cggtggacat ggtcgtcgac
      721 gcgatcggcc cgacgatgca gcgctacttg gtgggtgata tcacgcgctg acggctaccg
      781 gccggcgcgt tcgattcagc tggcgattcg cccgacctgg atctgtccct cgggcgtgac
      841 gcggacctga tacaccggca ccgacgcccg tggatcgtcc aggcaggaac cgtcctcgag
      901 cgcaaaagcc tgcttcagga gcggcgattg gaccgtggca cggccgccgc ggtccccgac
      961 gatcccacgc gacatcaccg ccgccccgga aaacgggtcg acgttaccga cggcgtgcac
     1021 cgacccgtcg tcgagccgga acagcgccgc ctgcgtgccg tcatcgagga gcacaccgac
     1081 accacgaccc ggaatgaggt ggtcgtaagc gcaggcggtg gtccacacct ggatgtcgtt
     1141 gagaagtgtc atcgttgctc cttgcttctt gcgcgccaca tctgcgacct gccggcatcg
     1201 tcgttgcggt tcacgggcgg acccgcggca taccaatgga cacagggact tttcgcccgg
     1261 cacgctcggt gaatgtgacc gtcgagtcga cggcgtccgg ggcgttgacg aaggagacga
     1321 accgcgacag cttgtccgga tcttccagca cgcccttcca ttcgcactgg tagttctgta
     1381 cgtggcgctc catcgcggcc tcgaattccg cggccagacc tagggagtcc tcgcacacga
     1441 cctcacgcac gtgatccagg ccgccgtcca gcgattcgac ccacggtgcg gtgcgctgca
     1501 ggcgctcggc cgtgcggatg tagtacatca ggaaccggtc gacgtagcgg acgagcgttt
     1561 cggtatccaa gtcgctgacc agcagctgag cgtgcttggg ggtcatgccg ccattgccgc
     1621 ccacgtagag gttccagccc ttttcggtgg cgatgacgcc cacgtccttg ctgcgggcct
     1681 cggcgcactc gcgcgcgcag cccgagacgc cgagcttgat cttgtgcggc gcccgcaggc
     1741 cccgataccg cagctcgaga tcgatggcca gctgcaccga atcctgctgg ccgtagcggc
     1801 accagtcact gcccacgcag ctcttcacgg tgcgcaacgc cttgccgtag gcgtggccgg
     1861 attccatgcc gccgtcgacc agccgcttcc agatctgcgg cagctggtcc acccgggcgc
     1921 cgaacaggtc gatccgctgg ccgccggtga tcttggtgta aagcccgaaa tcctgcgcga
     1981 tctggccgat cagaatcagg tgctcgggct tgatgtcgcc gccggggacc cgcggcacca
     2041 ccgagtagct gccgttcttc tgaatgttgg ccaggaaatg gtcgttggaa tcctgcagcg
     2101 aggcttgctc gccgtccaga atgtggtcgg acccggtgga cgccagaatc gaggcgacca
     2161 cgggtttgca gatgtcgcaa ccctttccgc ggccgaagcg ctccagcaga ccggagaacg
     2221 tccggatttc ggtggcggag atgatctcga agagctccgc ccgcgactgg ctgaagtgct
     2281 cacacagcgc cttggactgc tccacgccct cggcttccag cagctgcttg agcaacggca
     2341 cgcacgaccc gcacgaggtg ccggccgccg tgcacgactt gagcgccgga acgtcggcgc
     2401 agccgccggc aatcgcgcac ttcagatcac ccttggtgac gttgttgcac gagcagatct
     2461 gggccgagtc cggcagcgaa ccaatgccca gagccgctac gccatccgac tgcgccggcc
     2521 cgatcaacgc caccggatcc cccggcagct cgctgccgac catgggccgc agcaccccgt
     2581 acgacgaggc gtcgcccacc agcaccccgc cgagcagggt tttggcgtcg tcggacagca
     2641 caagtttggc gtaagtccgg ttcaccgcgt cgttgatggc gacttccaga cagttctccg
     2701 tcgcgcccat ggcgtcgccg aagctggcga cgtcgacgcc cagcagtttg agcttggtcg
     2761 acagatccgc ctcgggaaac tccgcggtgc cgtccagcaa ccggtcggcc accacctcgg
     2821 cggaggtgta accgggcccg accaggccgt agcaccgccc cccgatagcg gccacctcac
     2881 cgaccgcgta gatgtcggga tcgcttgtcc ggcaagacag atcggtgagg acgccgcccc
     2941 gctcggcgcg ggtcaacccc gcggccaccg ccaactcgtc acgcggccgg atgccggccg
     3001 cgaaaatcac cacgcccgcg tcgatgagct caccgtcgct cagccgcacc cgcagcgaag
     3061 tcgggccatc ggacgcggca acggattcga tcgattcggt gcccgtcccg acgtgcaccg
     3121 cgataccgag gtcgctgacc atcctggcca acagcgcgcc gccggcctcg tcgatctgtt
     3181 gggccatcaa ccgcggcatc atctcgacaa cgtgggtctg caacccgaac tggcgcagcg
     3241 cattagcggc ttcgagtccc agcaggccgc cgccgatgac cacccccgcg gtggcgcggc
     3301 cgccgtccat cgtgcgctgc gcatcggccc ggatggcgtc gaggtcgtcg agagtgcggt
     3361 agacgtggca cgcgggcagg tcatggccag gcaccggagg gacgaacgcg taggacccgg
     3421 tggcgagcac cagcgcgtcg aattggtggc gttgtccgtc ggcggtgacc acagactttg
     3481 tcgcgcggtc gatttcggtc acccgcgtgt tgagcaccag gcgcacccgc tcgtcgccgg
     3541 cgtagtggtt cccgggcagt gccagcaacc cgcgatccca gctgtcggtg taagaagtca
     3601 agccgacccg gtcataggcg gcgtcggcct cttcggccaa aaccgtgata caccagactc
     3661 cctcggtgtc gcgcgcgcgg agcgcctcga cgaatcggtg acccaccatg ccgtgcccga
     3721 ctacgacgac gtgacgcgga gcacagtgcg cacgcgcagt ttcggatggg gccatgtcac
     3781 gagggtaggg gtatgaaatt accggggaat cgcggaatgt gacaccggca tgacagcgcc
     3841 ctcacacctc acaagtcgcg ctgtgaaact gttcgctgcg ggcgaacgcc cacgtggaac
     3901 tttagcgtgg tcgactcatg tttctagaga tagccgaccg ataaggcatc ggtcacagct
     3961 aactcacacg aaagcacaag gatccgaaag gatcgaaaag gagaagccat gactaacctc
     4021 gcagtgtggt cgcgatcggc ccgggacacc gaccggtggt tgcgcgactt tttcggcccc
     4081 gccgcggcgg cggactggta taagccgctg aacagcggtt tcagtccggc agcggagatc
     4141 gtcaaggacg gcgacgacgc ggtggtgcgg ctcgagctgc caggcgtcga cgtcgagaag
     4201 gacgtgaacg tcgaggtcga caagggccgc ctggtagtcc acggcgaacg ccgcgacgag
     4261 cgcacggagg aggagaacgg ccgcaccctg cgcgagattc gttatggatc gttccgccgg
     4321 tcgttcaagc tgcccgcgca cgtcaccagc gaggccgtca cggcttcgta cgacgacggt
     4381 gtgctcaccg tgcgggtcac cggtgcccac caggacccgg cccagccgca ggcacagcgc
     4441 atcgagatca ccaagtaatc cggagtaaac aacgggaatc cggcggggcc atcggctccg
     4501 ccggattcgc cgcgcctgga cacctgggca tcatcaactg tgttgacgcg caacacattc
     4561 gccggccccg gtagatcagg cccagcgcgc cagcaattcc cgggcccgaa ccgccagcgc
     4621 ggcctgcaga aagggcccga agctgatccg gccgacaccc agcgggccga acgatgccgg
     4681 gtcatccagc tcgggcagcg cgatcgcgtt gatcggcagc gggagctcgg tggccaagcg
     4741 gcgcaatgtc tccgggtcgt ggcgacccac cggatacaag acgtcggcac ccgcggcggc
     4801 ggcctcggtc agccgcgcca ccgcgcgctc gacgcgatcc gactgttcac cgtcctgccg
     4861 caggaacacg tcggtgcggg cgttgatcac gacatgcacc ccggccgcgt cggccgccga
     4921 ccgcagcgcg cccaccagct gggcatgttc accggctgac cgcagcctct tgccctcgga
     4981 gtgcacggtg tcctcgatgt tcagcccgac ggcacccacg ctcagcaggc cctcgatcag
     5041 gcgcccggcc ggctgcccgt aaccggactc gatgtccgcc gacaccggca cgtcgaccgc
     5101 ggcggtgatc tgagcgacgc gggccaacac gtcgtcgaac gacatgccct cgccgtccgg
     5161 tttgccgatc gaatcggcga ggggatgact gcccacggtg agagcgacga aacccgcgtc
     5221 cgtggccagg cgcgccgacc aggcatccca taccgtcggc aggaccaccg ggttgcccgg
     5281 ctgatgcagc tcaagcagcg ccctcgcacg ttcggtaatg gtgtgacctg ccatgggcat
     5341 ttactccctc gatccttcgg ggtgacctgg ctgccgccag gctcctgagg tggatagtat
     5401 ttagggcgta ctgtgatctg taacaccgtc tgcccgagga gatccgttga ctagctctgc
     5461 cggcacgagc gaactcgccg aactgcgcga tctcatgggc ggtttgcgga agtgcgtgca
     5521 ctccttgaag gcgcgatacg gcgacagccc ggcgatgcgt cgcatcgcca tcgacgcaga
     5581 tcggatcctg tccgacgtcg aattgctcga gaccgacctc tccgaattgg acctcgcacg
     5641 cgcgaccgtc atgcagtccg gcgagaagat cgcgattccc gacacccagt acgacagcga
     5701 cttctggcgc gacgtcgacg acgaaggtgt cggaggtcac gcccggtcct gacaacccaa
     5761 gccccccgaa gataaagggg gtctctctgt gtacccttcg acctgacagc ccgttcgaag
     5821 aggaacacta gatgagcgca cctacggcga accgtcctgc gtccggcgtt ttctcacctg
     5881 gacgtgccga aatagcacag cgaaccctcc gcaccgaccg atggtggcaa gcaccgctgc
     5941 tgaccaacat cggcctgctg gcgttcatcg tttatgcgac cacccgggca ttttggggca
     6001 gcgactactg ggttgatcaa taccactatc tgacgccgtt ctattcgccg tgcgtgagtg
     6061 cgtcgtgcga gccgggcgcc agccacctgg gtgtttggtt cggtcatttc ccgtggtgga
     6121 tcccgttggg atcgctggta ttacccttcc tgctgggttt ccggcttacc tgctactact
     6181 accgcaaggc ctactaccgg tcggtgtggc agtctccgac cggatgcgcg gtgcccgaac
     6241 cacgtgtcca ctacaccggc gaaacacggc tcccgctgat tattcagaac agccaccgct
     6301 acttcttcta cgtcgcgctg gtggtctcgc tgatcaacac ctacgacgcg ataatggcgt
     6361 ttcattcgcc gtcgggtttc ggattcggtt tgggcaacgt cattttggtc accaacgtgc
     6421 tgttgctgtg ggcttacacc ctgtcctgcc attcgtgccg gcacgtcacc ggcggccgcc
     6481 tcaagcattt ctccaaacac cctgtgcggt actggatttg gacacaggtc agcaagatca
     6541 atacccggca catgctgttc gcgtggatca cgttggggac cctgtcactg accgacttct
     6601 acatcatgct ggtggccagc ggaaccatcg cagacttgag atttgttggc tgacaatcca
     6661 ttcaggattg cccagagaga gctgagcgag gtttcatggt tgaggtcgaa cggcgctcct
     6721 acgacgtcgt cgtgatcggt gccggcggcg cggggctgcg tgcggtcatc gaagcgcgcg
     6781 aacgcggcct caaggtcgcg gtggtgtgta agtccctgtt cggtaaggcg catacggtga
     6841 tggccgaggg cggctgtgcg gcctcgatgg gcaacaccaa cccgaaagac aactggaaga
     6901 cccacttcgg cgacacgatg cggggcggga agttcctgaa caactggcgg atggccgaac
     6961 tgcacgccaa ggaggcgccg gaccgggtct gggagctgga gacctacggc gcgctgttcg
     7021 accgcaccaa agacggcaag atcagccagc gaaacttcgg cgggcacacc tatccgaggc
     7081 tggcgcacgt cggcgaccgc accggcctgg agctgatccg caccatgcag cagaagatcg
     7141 tgtcgctgca gcaggaggac ttcgccgagc tcggcgatta cgaggcgcgg atcaaggtgt
     7201 tcgccgaatg cacgatcacc gagctgctca aagacggggt gcggatcgcc ggagccttcg
     7261 gctactggcg cgaaagcggc cggttcgtgg tgttcgaggc ccccgccgtg gtgttggcca
     7321 ccggcggcat cggcaagtcc ttcaaggtga cctcgaactc ctgggagtac accggcgacg
     7381 ggcacgcgtt ggcgctgcgg gcgggcgcct cgctgatcaa catggagttt gtccagttcc
     7441 acccgacggg catggtgtgg ccgccgagtg tgaaagggat ccttgtcacc gaaggtgtgc
     7501 gcggtgacgg cggggtgctg aagaattccg acggcaagcg gttcatgttc gactacatcc
     7561 cgcccgtgtt caagggccag tacgccgaaa ccgagcaaga agccgaccag tggctcaagg
     7621 acaacgactc ggcccgccgc acccctgatt tgctcccgcg cgatgaggtc gcacgcgcga
     7681 tcaactcgga ggtcaaagcc ggccgcggca ccccacacgg cggggtctac ctcgacatcg
     7741 cgtcccggtt gacgccggcc gaaatcaaac gccggctgcc gtcgatgtac caccagttca
     7801 tggagctggc cgaagtcgac atcaccaagc agccgatgga ggtcggcccg acgtgccact
     7861 acgtcatggg cggtgtcgag gtcgaaccgg acaccggggc cgcgacggtg gccgggctgt
     7921 tcgccgccgg cgagtgctcc ggcggcatgc acggttccaa ccggctgggc ggaaattcgc
     7981 tgtcggacct gctggtcttc ggtcggcggg ccggcctggg cgccgctgac tacgtgcggg
     8041 cgctgagtag ccgcccggcg gtcgaccagg atgccatcga cgcggcggcc aaacgggcgc
     8101 tggacccgtt cgagggaccc agcaacggcg ccgccgccgc cgagaacccc tacacgttgc
     8161 acctggaatt gcagcagtcg atgaacgacc tggtcggcat catccgcaat gcggacgaaa
     8221 tctcgcaggc cctcgaccgg ctcgacaaac tccgcgagcg gttcaagaac atccaggtcg
     8281 aggggcaccg gcagtacaac cccggctgga acctggccat cgacctgcgc aacatgctgc
     8341 tggtcagcga atgcgtggcc aaggcagcgc tggagcgcac cgagagccgc ggtggacata
     8401 cccgcgacga tcacccatcg atggactcgt catggcgcaa gctgttgctg gtctgccggg
     8461 ccgccggcgg tgacccggtg attccggaca tcgccgtcac gcgagaagac caggtgccga
     8521 tgcggtccga tctgctggaa ctcttcgaga tctccgaact tgagaagtac tacaccggcg
     8581 ctgagctggc cgaacatcca ggacggagag gctgatgacc tacaacgcaa ccatgcgggt
     8641 gtggcgcggc gacgaagccg gcggcgcgct ggaggacttc aaggttgagg tcaacgaggg
     8701 cgaagtggtg ctggacatca tccaccgcct gcagcagacc cagacgcccg acctcgcggt
     8761 gcggtggaac tgcaaggcgg gtaagtgcgg atcgtgctct gcggaggtca acggccaacc
     8821 gcggctgatg tgcatgaccc ggatgtccac attcgccgag gacgaggtgg ttaccgtgac
     8881 gccgctgcgg acgttcccgg tgattcgtga cctggtcacc gacgtctcgt tcaactacga
     8941 gaaggcgcgc gagatcccgt cattcgcgcc gcccaaggaa ctgcagcccg gcgaataccg
     9001 gatggcgcag gtggacgtcg cgcgctcgca ggagttccgc aagtgcatcg agtgcttcct
     9061 gtgccaaaac gtctgccacg tggtgcgtga ccacgaggaa aacaagaagg cgttcgccgg
     9121 cccgcgattc ctgatgcggg tcgccgagct ggaaatgcac cccctggaca cacgggaccg
     9181 gcgcaatcag gcgcaggagg agttcggcct gggcttctgc aacatcacca agtgctgcac
     9241 cgaagtgtgc cccgaacaca tcaagatcac cgacaacgcg ctgatcccga tgaaagagcg
     9301 ggtcgtcgat cgcaagtacg accccgtggt gtggctgggt aacaagctgt tccgtcgctg
     9361 agggcgctaa gcctcggtta tttccggctg cgcacctggt cgcgcgttgc ggacggcttc
     9421 tacttcctca gatcaaccgt tgaacccggg taggccgaac agcagtccgg cggcgccgcc
     9481 ggcaccggcg agagccaaga acccgccggc gttcgacccg gggttgccac cgttaccgcc
     9541 gtcgccgatc aacacggcgt tcccgccgtt cccgccagcc ccgggccccc cggcgaggaa
     9601 gaagttgacc ccgccttcac cgccgttgcc gccgttgccg accaccccgg cccggccccc
     9661 gaccccgccg gccccggaag tggtcgcgcc cgagccaccg gtgccaccga cgccgccgga
     9721 gccgatcagc ccgcggccat cgccgccggc cccgccggcc ccaccgacgc cgttgctgct
     9781 aggcccgccc gtgccgccga ccccgcccga gccgaccacc atggcggagt tgccgccgac
     9841 cccgccgacc ccaccgttgc ccacgctcgg accgccgacc ccgccgagcc cgccggagcc
     9901 gaacagcccg accgcgtcgc cgccgacccc gccggcccca ccggtggtga caccctggcc
     9961 gccggccccg ccaaccccgc ccgcgccgaa gagcaggcca ctggccccgc cgggcccgcc
    10021 ggccccaccg acggttgtgc cggccccgcc ggccccgcc
//
LOCUS       DASSGQ010000084        12135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_552888,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000084 DASSGQ010000000
VERSION     DASSGQ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12135
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552888"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..108
                     /locus_tag="VFI55_03825"
     CDS             <1..108
                     /locus_tag="VFI55_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7073197.1"
                     /translation="LTRLREDIVTPVFSCYVSHRIRRAAAKLAGRLIER"
     gene            complement(168..1160)
                     /locus_tag="VFI55_03830"
     CDS             complement(168..1160)
                     /locus_tag="VFI55_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7073198.1"
                     /translation="MDSARAKKKLVIGASGFLGSHVTRQLVAAGADVRVMLRHNSSTK
                     GIDDLDVERCYGDVFDDDALRAAMTGCDVVYYCVVDARMWLRDPAPLFRTNVEGLRHV
                     LDAALDADLTKFVFTSTTATLAISDRRVVTEDDAHNWDHGGAYVEARVAAEKMLMSYV
                     QDKGLPAVAMCISTTYGPGDWAPTPHGSLIALVATGRFPFYFGYSAEVVGIEDAARAM
                     LLAAEHGRIGERYIISDRYMSVRALHEIAATAVGRRPPRIGIPLSIMLAGARVNDVMA
                     RLLGRDLPFAYAGMRMAELMSPLDHSKAERELGWTPEPVDDSIRKAALWFASRN"
     gene            complement(1172..2143)
                     /locus_tag="VFI55_03835"
     CDS             complement(1172..2143)
                     /locus_tag="VFI55_03835"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7073199.1"
                     /translation="MKQAQLGELEVGRIGLGAMGMSVAYSGAGSDDAESIRTIHRAID
                     LGSTLIDTAEVYGPYINEELVARALRDRRDRVTLATKFGVISHTGRDVLDSSPANVRI
                     AVEGSLQRLATDHIDLYYQHRLDRETPIEDTVGALAELVTAGKIRYIGLSEVGVNTIR
                     RAHAVHPITAVQSEYSLWTRDPEDGVLDVLRELGIGFVAYSPLGRGFLAGAIRSANEL
                     ADSDYRKTNPRFFDENFRHNLNSANELRAIAADVGATPAQVALAWLLAKGSDIVPIPG
                     TKRVARLEENVAADAVELSPDQLARLDELTPPVGGHHAEAQMGWIDR"
     gene            2198..3526
                     /locus_tag="VFI55_03840"
     CDS             2198..3526
                     /locus_tag="VFI55_03840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET7073200.1"
                     /translation="MQDTARPDRNLAVDFCRVSGVVLIVLGHWLAGSVTYHNGSFGRQ
                     NPLVDIPWTQWLTWPFQAVPTFFLVAGYAGAVSWTHRRQTDGVSRQTWLRHRLARVLG
                     PTTVYVVLVSAMVAVAVGRVAGSTLEYAGWAIAMHLWFLAVYLVVVSLTPIAIAAQRR
                     WGLVVPMVLAMGVVAVDALSIGGHAHDLRRLNYLLCWGALYQLGIAWHGGLLAHRRAM
                     LLAVGSAIALALAIGVGPYPVSMIGVPGQTLDNTTPPTAAMLAFGYAQAGIVIAVAPA
                     LNRALRAGLVKRVLSVANNNVMALYLWHMIPVVLVAIVGYPAGLLPQPPEGTAQWWLA
                     RLEWVVILGLVAAIEMVLLWWGRRLFAAPLTMLGIPVTDRWTEPIMLAGVAMAAYGLS
                     FLAAEGFAPDGHFAWMTALVFAAGVVLVALLPTKVPRRSVVSAPEIGMTP"
     gene            complement(3551..4201)
                     /locus_tag="VFI55_03845"
     CDS             complement(3551..4201)
                     /locus_tag="VFI55_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7073201.1"
                     /translation="MSAMPDVEPPNRLERRKQRTRAALVRAAQRLIAEGKVNVPVLEI
                     TRAADVGMGSFYNHFDSKEQLFEAAIADVLDAHGAMLDRLTAPLDDPAETFATSFRLT
                     GRLFRRRPEESEVLLANGPALLASDRGLAPRALRDIKAAADAGRFSVDDPELALAMAG
                     GALLGLGKLLRDNPDRDDARAADTVTENVLRLFGLSAEEARVICQRPLPDDAFAET"
     gene            4303..5433
                     /locus_tag="VFI55_03850"
     CDS             4303..5433
                     /locus_tag="VFI55_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7073202.1"
                     /translation="MNLTNNPHRDLHSEHGALRGEHPGRSRNPVIKVADLAWLEFEKP
                     DLARAEKFARAFGFHTAQHGPAELLLRGTQAGGPSVILRRGPRTRFAGAAFRAHDEVD
                     VRRLADKSGAATRPLPENIGGLSVDLIDPSGMLVRVVAGMHELPELPDQQPHVFNFGH
                     DTQRTNTGQRPPLVPARVQRLGHLVVQSTKYRETLDWYLDNLGMIVSDFQFFPGQRDR
                     GPTMSFIRCDRGATPADHHTLALALGPANRYVHSAYQVSDLDALAAGGEYLRERGYFR
                     SWGIGRHIQGSQIFDYWRDPDGFLVEHFTDGDLFDNTLEPGWAPFTASGLAQWGPRAT
                     KDFLGTDLKSAGHEVISIISALRASNEFDVRRLAGLLKVPTS"
     gene            5430..6389
                     /locus_tag="VFI55_03855"
     CDS             5430..6389
                     /locus_tag="VFI55_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET7073203.1"
                     /translation="MTVSVLHTASETGHAWWLKTPDGAARIDTTATTTAALLADRTAF
                     ESAISGAANADTVAVHSLKLISPVTRPCRVVAQMTNFESHVKDAGMDPASIPLTFFRK
                     ASASINGPFDDIVKPPHVQLLDYEVEIGLVIGRPIPVGTNISDANLADFIAGLVVAND
                     VSARDIQLPQTQFYEAKSYPTFTPVGPELVLLTADELNRFGELRLRLTVNGAERQNAL
                     VNGDMLYRPLEALQSLTQFQELAPGDLVLTGTPAGTALSAPPKPVQMVGNLLPPALKW
                     KMFFKRQAGNPNYLRCGDVVEASVATDDGAIDLGTQRTAVRFA"
     gene            6386..7957
                     /locus_tag="VFI55_03860"
     CDS             6386..7957
                     /locus_tag="VFI55_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HET7073204.1"
                     /translation="MTDNAIQRVPVVPVVPVVPVVIVGAGPTGVTAATLLAQYGLDCL
                     VLDRWPGVYPQPRAVHLDDEIYRVIARLGIAEEFAAISRPTLGLRLLDNRSRPSQRFG
                     VLAEFHRDPSRSPNGFPQANMFDQPELEALLRANLKRFPNARLRGEAEVTELTEHGRC
                     IRVTFTDRSDGRVHHVDADYVLGCDGANSVVRAEIGSAMRDLKFQQRWLVIDVAASSD
                     LHQWDGVHQVCDPVRAATYMRIGPSRYRWEFQLLAGETADDFGTLDALRPLIAPWTAD
                     VADCELTLLRVTEYTFRAQVADRWRRGNVFILGDAAHLTPPFVGQGMGAGVRDAANLA
                     WKIAGVHDGTLAAAVLDTYEQERKPHTRYMIRLALGVGWAMTGGGRVGDAARRVLLPR
                     LRLIPGLRDKVVDSTTPALRASALVCKSRRPRALAGTLCPNPLLPNGQRLDDVVGTGF
                     ALVTRIGPSAAEEASLRRRGVFVLIAQPHGALDNWLRRGHATAALVRPDRTVLRAARD
                     VTALCAWTVGALRGG"
     gene            complement(7963..9291)
                     /gene="efeB"
                     /locus_tag="VFI55_03865"
     CDS             complement(7963..9291)
                     /gene="efeB"
                     /locus_tag="VFI55_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HET7073205.1"
                     /translation="MVDDELAESADDTAKDSGAPADTTGVSRRRMLGGAMLAGLTGTA
                     VGAVGGGFAGYATAATHDSDSAAGDDTVDLRRSYPFYGQAHQGGIATLPQHYAVFMSF
                     SLAAGAGRAGLQTLLARWSAAAAVLQQGKPVGTVQPQVDVQPPTDTGEAYGLSPASLT
                     VTIGLGPSVFGDRFGLAARRPAAFTDLPPLNGDNLDPRLHGGDFSVQACADDPQVCYH
                     AVRNLARIGRNIVSPFWAVLGFGRASAGPGQHTPRNLLGFKDGTRNIASDAEYDRFVW
                     VDNSDQQWMNGGTYQVIRKIRMLLETWDVDRIGNQQKIFGRTKEEGAPLSGKAEFDTP
                     NFAANDADGDPLIDPKSHVGVAARENNDGIMIRRRSYNYTDGLDANGQLNAGLLFISY
                     QKDPQDFIRLQNRLGAHDLLNEYIRHIGSAIFAVPPAPAEGHYIGQSLFS"
     gene            complement(9294..10469)
                     /gene="efeO"
                     /locus_tag="VFI55_03870"
     CDS             complement(9294..10469)
                     /gene="efeO"
                     /locus_tag="VFI55_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HET7073206.1"
                     /translation="MLSVTACGHSSGTSSPSKPGQHGGSSVVNVSMANNGGKDGCALD
                     TTHVPAGPVTFTVVNTSAPGITEVELLRDQRIVGEKENLAPGLDPVSFTVTLDGGSYQ
                     LYCPGASAEYQTLTVTGQAPAAPTGTVASVLSQGTKDYAAYIVNQIGQLNGGVKALDA
                     AVQSGNLDAAKTAYAKARLFWERSESSVEGFVLPGFAAGDNAGSLDYLIDMRESTPVD
                     AKVGWKGFHAIERDLWRGGAITPGTKALSTELVGNVGKLDATVATLQYKPEDLANGAS
                     DLIEEVQNTKITGEEEAFSHIDLVDFSGNVEGAQQAYASLRTGLEKIDGNLVTQIDQQ
                     FQTVLSALDGYRDPSALGGYRLYTPELRATDASKLTAVIQPLHQSLSTVAQKVVSAN"
     gene            complement(10507..>12135)
                     /gene="efeU"
                     /locus_tag="VFI55_03875"
     CDS             complement(10507..>12135)
                     /gene="efeU"
                     /locus_tag="VFI55_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HET7073207.1"
                     /translation="GVFGVFIGTFLIGLREGLEATLIVSIIGAFLKRNSQSTRPMFAG
                     VAAAVLISASVGVGLDLLSATLPQASQEMMETVINAIAVVFVTSMIIWMNRNAARLKG
                     ELERDAQKALNQGGALALAAMAFLAVLKEGFETSVFLLAAAETSYGNRWLALLGGVAG
                     IAVSIALGAGMYFGGLKLNLGRFFRVTGVFLVLIAAGLVTGALRAAHEAGWISIGQQQ
                     VLDFSAWIPSRSVLGALVTGVFGIPADPRLIEVLGWLLYAVPVLIVFLWPAGLAAAPR
                     IRCRLLAATSAALLIVAAVLAFAVPSGGDASGVHTRTVTEQDGHTSTVSLTPGPHGSM
                     LAIVPEGAANVRSIPLAAVGDQTVDGVWVRVWQGVVTSNGAPAGDGATALTLEQLLRM
                     TGDRLPVGVSAARTPGPFQAQLATSTVYTVLAQGDFVVSARAISNRTAVLTGGGLTAA
                     KTVSIGGLPTDWSTAGAEDASTAAQIAATDRNRAEGQLWKVWLPLVLAGFALACAVSA
                     LVALFSVRGDRARTEHEGNTIDSESQHPGKVPAS"
BASE COUNT         1943 a         4013 c         4010 g         2169 t
ORIGIN      
        1 ctaactcggc tgcgcgagga catcgtcacg ccggtgttct cgtgctacgt ctcccaccgc
       61 atccggcggg cagccgccaa gctcgccggc cggctgatcg agcggtagcg cgtcgatgac
      121 tgttggcacc atttttgaat acgggcctgc ggcgctggcc aatgcgacta gtttcgcgat
      181 gcgaaccaga gcgcggcttt gcgtatcgag tcgtcgacgg gttcgggcgt ccatccgagt
      241 tcgcgttcgg ccttgctgtg gtcgagcggt gacatgagtt cggccattcg cataccggca
      301 taggcgaacg ggaggtcgcg gcccagcagt cgcgccatca cgtcgttgac gcgcgcaccg
      361 gcaagcatta tcgacagcgg gatgccgatg cgcggcggcc gcctgccgac ggcggtggcg
      421 gcgatttcgt gcaacgcacg aacgctcatg tagcggtcgg agatgatgta tcgttcgccg
      481 attcgtccgt gttcggcagc gagaagcatt gccctggctg cgtcttcgat acccacgact
      541 tccgccgagt agccgaaata gaagggaaaa cgtccggtgg cgacgagggc gatcagtgag
      601 ccgtgcgggg tcggtgccca gtcgcccggg ccataggtgg tggatatgca cattgccacg
      661 gcgggcaggc ctttgtcctg gacatacgac atcagcatct tctcggcggc gacccgagcc
      721 tcgacgtacg caccgccgtg gtcccaattg tgcgcatcat cttcggtgac aacccggcgg
      781 tcgctgatgg cgagagtggc tgtggtgctg gtgaatacga acttcgtcag atcggcgtcc
      841 agggcggcgt cgaggacgtg gcgcagccct tcaacgttgg tccggaacag cggcgcggga
      901 tcacgcagcc acatccgggc gtcgacgacg cagtagtaca caacgtcaca accggtcatg
      961 gccgcccgca gggcgtcgtc gtcgaaaaca tccccgtaac agcgctcgac gtcgaggtcg
     1021 tcgatcccct tggtggagct gttgtggcgc aacatcactc gcacgtctgc acccgcggcg
     1081 accagctgcc gtgtcacgtg cgatcccaga aagccgctgg ctccgatcac cagcttcttt
     1141 ttcgcacgcg ccgaatccat atccgcctgc ctcagcggtc gatccatccc atttgcgctt
     1201 cggcgtggtg gcccccgacg ggcggagtga gttcgtcgag tctcgccagc tgatcggggc
     1261 tgagctcaac cgcgtcggcg gcaacgtttt cctcaagacg tgcgacccgc ttggtccctg
     1321 ggatgggcac gatgtcggaa cctttcgcca gcagccacgc cagcgcgacc tgagccggcg
     1381 tggcgccgac gtcggcggca atggcacgca gttcgtttgc gctgttgagg ttgtgccgaa
     1441 agttctcgtc gaagaagcgg gggttggtct tgcggtagtc gctgtcggca agctcattcg
     1501 cggagcggat ggcgccggcg agaaaaccac gtccgagcgg cgagtaggca acgaatccga
     1561 ttccgagttc acgcagcacg tccagcactc catcttcggg atcgcgggtc cacagcgagt
     1621 attccgactg caccgcggtg atcggatgca cggcgtgggc gcggcgaatc gtgttcacgc
     1681 cgacttcgga aagacctatg taccggatct ttccggcggt gaccagttcg gccagcgctc
     1741 cgaccgtgtc ttcgatcggg gtctcgcggt cgaggcggtg ttggtagtag aggtcgatgt
     1801 ggtcggtcgc cagcctctgc agggaacctt cgacggcgat gcggacattg gcggggctgc
     1861 tgtcgagaac gtcccggccg gtatgggaga tcacgccgaa cttggttgcc agcgttacgc
     1921 gatcgcgtcg gtcccgcagg gcgcgcgcca cgagttcttc gttgatgtag gggccataga
     1981 cctcggcggt gtcgatcagc gtgctgccga ggtcgatggc gcgatgaata gtgcggatcg
     2041 attcggcgtc gtcgctgccg gcgccggaat acgcgaccga catgcccatc gcacccagac
     2101 cgatgcgtcc aacttcgagt tcgccgagct gagcctgttt catggggcca ttctgcgccg
     2161 agccgcttcg cgcgacggga cgggtaccgt ggcgccagtg caagacaccg cgcgaccgga
     2221 ccgcaatctg gcggtggact tttgccgcgt ctcgggtgtg gtcctcatcg tgctggggca
     2281 ttggctggcc ggatcggtga cctatcacaa cggatccttc ggtcgccaga acccgctcgt
     2341 cgacatcccc tggacccagt ggctgacgtg gcccttccag gcggtcccga cgtttttctt
     2401 ggtggccggc tacgccggcg cggtgtcttg gacgcatcgg cgccagaccg acggcgtgtc
     2461 gcgccagacc tggcttcggc atcggctggc ccgcgtgctg ggcccgacaa cggtatacgt
     2521 cgtgctggtg tccgcgatgg tggcggtggc agtcggccgg gtggccggtt cgactctgga
     2581 gtacgcgggt tgggcgatcg cgatgcacct ctggttcctg gccgtatacc tggtcgtggt
     2641 gtcgctgacg ccgattgcga ttgcggcaca acgccgttgg ggtcttgtgg tgccgatggt
     2701 gctggcgatg ggcgtcgttg cggtggatgc cctgtcgatc ggcggccatg ctcacgacct
     2761 gcgccggctg aactatctgc tgtgctgggg cgcgctctac cagcttggaa tcgcttggca
     2821 cggcgggcta ttggcccatc gcagagcgat gctgctcgcg gtcggctcgg ccatcgcgct
     2881 ggcgctggcg atcggggtgg ggccctatcc cgtcagcatg atcggtgtgc ccggtcaaac
     2941 cctggacaac accacgccgc ccaccgcggc gatgttggcg ttcgggtacg cgcaggccgg
     3001 aattgtgatc gcagtcgcgc ccgcgctcaa ccgcgcgctc cgcgccggtc tcgtcaaacg
     3061 cgtgctatcc gttgccaaca acaacgtgat ggccctctat ctatggcaca tgattcccgt
     3121 ggtgctggtg gccatcgtcg gctatccggc cgggctcttg ccccaaccac ccgaagggac
     3181 ggcccagtgg tggctggcca ggctggaatg ggtggtcatc ctcggccttg tggcagcgat
     3241 cgaaatggtg ctgttgtggt ggggacggcg cctcttcgcg gcgccgctta ccatgctcgg
     3301 cataccggtc accgatcgct ggaccgaacc gatcatgctg gcgggcgtgg cgatggcggc
     3361 ctatggcctg tcattccttg ccgccgaggg cttcgcgccg gacggccatt tcgcgtggat
     3421 gaccgcgttg gtctttgccg ccggagtggt tctggtggcg cttctcccga cgaaggtccc
     3481 ccggcgatcg gttgtctcgg cacccgagat cgggatgacc ccctgacttg ccatcgtcgg
     3541 tgatggtggc tcaggtctcg gcgaacgcgt cgtccggcaa tgggcgctga cagatcacgc
     3601 gtgcctcctc ggcgctgagc ccgaacagcc gcaggacgtt ttcggtgacg gtgtcggcgg
     3661 cgcgggcgtc gtcgcgatct gggttgtcac gcaacagttt tccgagcccc agaagcgcgc
     3721 cgccggccat ggcgagggcg agttcggggt cgtcgacgct gaatcggccc gcgtccgcgg
     3781 cggccttgat gtcgcgcagc gcacgcgggg ccaggccgcg atcggacgct agcagcgccg
     3841 gcccgttggc caataggacc tcgctctcct ctgggcggcg acgaaacaat cggccggtca
     3901 gccggaaact ggtggcgaat gtttccgccg ggtcgtcgag gggcgcggtg agccggtcca
     3961 gcatcgcccc gtgggcgtcc agcacgtcgg cgatcgcggc ctcgaacagc tgctccttgc
     4021 tgtcgaagtg gttatagaag gaacccatcc cgacgtcggc ggcccgggtg atctccaaga
     4081 ccggcacgtt gaccttccct tcggcgatca accgctgcgc ggctctgacc aacgccgccc
     4141 tggtgcgctg cttgcgccgc tccaggcggt tgggcggctc tacgtccggc atcgcgctca
     4201 tcctcagcag tatgcatcac ttttgaggat ttcgtcagta acccttgact gaacgttgcc
     4261 tcgtatgtga cgatttcgtc agttacccct ctggagatgg tcatgaatct gacgaacaac
     4321 ccccaccgcg acctacacag cgagcatgga gctctgcgcg gcgaacaccc cggtcggtcc
     4381 cgcaacccgg tgatcaaagt cgccgatctt gcctggctgg agttcgaaaa gcccgacctg
     4441 gcgcgagctg agaagttcgc ccgggccttc ggcttccaca cggcacagca cggccccgcc
     4501 gagttgctgt tgcgcggcac ccaggcgggt ggaccgagtg tgatcctgcg tcgtgggccg
     4561 cggacacgat tcgccggtgc ggcgtttcgg gcgcacgacg aggtcgacgt gcggcgcctg
     4621 gccgacaaat ccggtgccgc cacgcggccg ttgcccgaga acatcggcgg cctgtcggtg
     4681 gacctgatcg accccagcgg catgctggtc cgcgtcgtcg ccgggatgca cgagttgcct
     4741 gagctacctg atcagcagcc ccacgtcttc aatttcgggc acgacacaca gcgcaccaac
     4801 accggacaac gtcccccgct cgtacccgca cgggtgcagc gcctgggcca cctggtggtg
     4861 cagtcgacga agtaccgcga gacgctcgac tggtatctgg acaacctggg gatgatcgtc
     4921 agtgacttcc agttctttcc cggccagcgc gaccgcgggc cgacgatgag cttcatccgc
     4981 tgcgatcgcg gggcgacacc ggccgatcac cacacgctgg cgctggcgct gggtccggcc
     5041 aaccgttacg tccactcggc gtatcaagtc agcgatctcg acgccctggc cgccggcggc
     5101 gaatacctca gggagcgggg ctatttccgg tcctggggaa tcggcaggca catccagggc
     5161 agtcagatct tcgactactg gcgtgatccc gatggcttcc tggtcgagca tttcaccgac
     5221 ggcgacctgt tcgacaacac cctggagccc ggctgggcgc cgttcaccgc gtccggactg
     5281 gcccagtggg ggccacgggc caccaaagac ttcctcggaa ccgacctgaa atctgctggc
     5341 cacgaagtaa tttcgataat ctcggcgctg cgcgcaagca acgagttcga cgtccgccgc
     5401 ttggccggcc tactgaaagt gccgacgtca tgaccgtctc cgtgctgcac accgcctctg
     5461 aaaccggcca tgcctggtgg ctcaagacgc ccgacggggc cgcaaggatc gacaccaccg
     5521 ccaccaccac cgccgcgctg ctggccgacc ggaccgcgtt cgaatccgcg atctcgggcg
     5581 ccgccaacgc cgacaccgtc gccgtgcaca gcctgaaatt gatctcgccg gtgaccagac
     5641 cgtgccgagt ggtggctcaa atgaccaact tcgagtcgca cgtcaaggac gcgggcatgg
     5701 accccgcgtc gatcccgctg accttcttcc gcaaggcgtc ggcgtcgatc aatggtccgt
     5761 ttgacgacat cgtcaaaccc ccgcacgtcc agttgctcga ctacgaggtc gagatcggat
     5821 tggtgatcgg gcggccaatc ccggtcggca ccaatatttc cgacgcgaac ctggccgact
     5881 tcatcgccgg actggtcgtc gccaacgacg tctctgcgcg tgacatccaa cttccacaga
     5941 cccagtttta cgaggccaag tcctatccga cgttcacccc ggtcggtccg gagctggtct
     6001 tgttgacggc cgacgaactc aatcgattcg gcgagttgcg gcttcggcta accgtcaacg
     6061 gcgccgagcg gcaaaacgcc ctcgtcaacg gtgacatgct gtatcggccg ctggaggcac
     6121 tgcaatcgct cacccaattc caggagctcg ctcccggtga tctggtgctc accggcaccc
     6181 cggccggcac cgcgctgagc gcgccgccga agccggtgca gatggtcgga aacctgttgc
     6241 caccggctct gaagtggaag atgttcttca agcgccaggc cggcaacccg aactatctgc
     6301 ggtgcggcga cgtggtggag gcgtcggtgg caaccgacga cggggccatc gatcttggaa
     6361 ctcagcgcac cgcagtgcga ttcgcgtgac cgacaacgct attcagcgcg ttcccgtcgt
     6421 cccggtcgtc ccagtcgtcc cggtcgtcat tgtcggcgcc ggaccgaccg gcgtcaccgc
     6481 cgccacgctg ttggcacagt acgggctgga ttgcctggtg ctcgatcgct ggcccggggt
     6541 ctacccgcag ccacgtgcgg tgcatctgga cgacgaaatc tatcgtgtga tcgctcgcct
     6601 ggggattgcc gaggagttcg ccgcgatttc gcgacccacg ctgggtttgc ggctgctgga
     6661 caaccgatcc cggccatcgc aacgtttcgg tgtcctcgcc gaattccacc gcgacccctc
     6721 gcgcagcccg aacggcttcc cgcaggccaa catgttcgac caaccggagt tggaggcgct
     6781 gctgcgggca aacctgaagc gcttcccgaa cgcccgactg cgcggggagg ccgaggtcac
     6841 cgagctgacc gagcacggtc gatgcatccg cgtcaccttc accgaccggt ccgacggccg
     6901 cgttcaccac gtcgacgccg actacgtgct gggctgcgat ggcgccaaca gcgtggtgcg
     6961 cgccgagatc ggctcggcca tgcgggattt gaagttccag cagagatggc tggtcatcga
     7021 tgttgccgcc agcagcgacc tgcaccagtg ggacggcgtg caccaggtat gcgacccggt
     7081 gcgggcggcg acctacatgc gcatcggccc gtcccgctat cggtgggagt ttcagctgct
     7141 ggccggcgaa accgctgacg acttcggcac cttggacgca ttgcggccgc tcatcgcgcc
     7201 ctggaccgcc gacgtcgcag actgcgagct gacgctgctg cgcgtgaccg agtacacctt
     7261 tcgcgcgcag gtcgccgatc ggtggcgccg cgggaacgtg ttcatcctcg gcgacgcggc
     7321 acacctcact cccccattcg tcggccaggg catgggcgcc ggcgtgcggg atgcggccaa
     7381 tctcgcctgg aagatcgccg gagtccacga cggcaccctg gcggcagccg tgctcgacac
     7441 ctacgagcag gaacgcaagc cgcacacccg ctacatgatt cgcctggccc tcggtgtcgg
     7501 ctgggcgatg accggcggtg gccgcgtagg cgacgcggcg cgccgcgtgc tgctgccccg
     7561 gctgcgactg attcccggcc tgcgggacaa ggtcgtcgat tcgacgacgc cggcgctgcg
     7621 tgcgtcggcg ctggtctgca aatcccgccg gccgcgcgcg ctcgccggca cgctgtgccc
     7681 gaatccgttg ctgcccaacg gtcaacggct cgacgatgtg gttggcaccg gattcgcgct
     7741 ggtcacccgg atcggaccca gtgcggccga ggaagcatcg ctgcggcgcc gtggagtctt
     7801 cgtgctgatt gcccagccgc acggcgcgct cgacaactgg ctgcggcggg gacacgccac
     7861 tgccgcgctg gtgcgtccgg accggacggt cctgcgcgcc gcacgcgacg ttacggcgtt
     7921 gtgcgcgtgg accgtcggcg cccttcgcgg cgggtgaagg cttcagctga acagactttg
     7981 cccgatgtag tggccttcgg ccggcgcggg cggcaccgca aagattgctg atccgatgtg
     8041 ccggatgtat tcgttgagta gatcgtgggc gccgagccgg ttctgcaaac gaataaaatc
     8101 ttgcggatct ttttgatacg agatgaacag caagccggcg ttgagttggc cgttggcatc
     8161 gaggccgtcg gtgtagttgt aggaccgtcg ccggatcatg atgccgtcgt tgttttccct
     8221 ggcagccacg ccgacgtgag acttcggatc gatgagcgga tcgccgtcgg cgtcgttggc
     8281 ggcgaaattc ggggtatcga attcggcctt gccgctcagg ggcgcgcctt cctctttggt
     8341 gcggccgaat atcttctgct gattgccgat ccgatcaaca tcccatgtct cgagcagcat
     8401 ccggatcttg cggatgactt gataggtccc gccattcatc cactgctggt cgctgttgtc
     8461 aacccacacg aaccggtcat actcggcgtc gctggcgatg ttgcgggtac cgtccttgaa
     8521 tcccaagagg tttcgtgggg tgtgctggcc ggggccggcc gaggcgcggc cgaatcccag
     8581 caccgcccaa aacggtgaca cgatgttgcg gccgatccgg gccaggttgc gcacggcgtg
     8641 gtagcagacc tggggatcgt cggcgcaggc ttgcaccgaa aagtcaccgc cgtgcagacg
     8701 gggatccagg ttgtccccat tgagcggcgg caggtcggtg aacgcggcgg ggcgccgcgc
     8761 cgcaagtccg aaacggtcac cgaacaccga cggccccaac ccgatggtga cggtgagact
     8821 ggcagggctc agcccgtagg cctctccggt atcggtgggc ggctgcacat cgacctgcgg
     8881 ctgcacggtt cccacgggct tgccctgctg caagaccgct gccgcagccg accagcgtgc
     8941 caggagggtt tgtagacctg cccggccggc gcccgcggcc agcgagaacg acatgaacac
     9001 ggcgtaatgc tgtgggagcg tcgcgattcc gccctggtgg gcctgaccgt agaacgggta
     9061 gctgcgccgc aggtccacgg tgtcgtcccc agcggccgag tcgctgtcat gcgtggccgc
     9121 ggtggcgtag ccggcgaacc cgccaccgac ggcacccacc gcggtaccgg taagccctgc
     9181 caacatcgcg ccgccgagca tgcggcggcg cgacacgcct gtggtgtcgg ccggcgcccc
     9241 gctgtctttg gcagtgtcgt cggcggattc ggccagctcg tcgtccacca tcgtcagttg
     9301 gccgaaacga ctttttgggc gaccgtggac aggctctgat ggagcggctg gatcacggcg
     9361 gtcagcttcg atgcgtcagt ggccctcaac tcgggcgtgt acagccggta gccgcccagc
     9421 gccgacggat ctcggtagcc gtccagtgcg ctgagcaccg tctggaattg ctgatcgatc
     9481 tgggtgacaa gattgccgtc gatcttttcc agccccgtcc gcagcgaggc gtaggcctgt
     9541 tgagcgcctt cgacgttgcc cgaaaagtcg accagatcga tgtggctgaa ggcctcctcc
     9601 tcaccggtga tcttggtgtt ttgcacctct tcgatcagat cgctggcacc gttggccaaa
     9661 tcctcgggct tgtattgcaa agtggcgacg gtggcgtcca atttgcctac gttgcccacc
     9721 aattcagtgc tcaacgcctt ggtgcccggg gtgatcgcgc cgcctcgcca gagatcgcgt
     9781 tcgatggcat gaaagccctt ccacccgact ttcgcatcga ccggggtgga ctcgcgcatg
     9841 tcgatcaggt agtcgaggct gccggcattg tcaccggcgg cgaatcccgg caggacgaag
     9901 ccctccacgc tggactccga acgctcccag aacagccgcg ctttcgcgta ggccgtcttc
     9961 gccgcatcga ggttgcccga ttgcaccgcg gcgtccagcg ctttcacgcc gccgttgagc
    10021 tgaccgatct ggttgacgat gtatgccgcg tagtccttgg tgccctggct gaggacgctc
    10081 gccaccgtgc ccgtcggcgc cgccggtgct tgacccgtga ccgtcaaagt ttgatattcg
    10141 gcgctcgcgc cggggcagta cagctgatag gaaccgccgt ccagcgtgac ggtgaacgac
    10201 accgggtcca ggccgggcgc gaggttctcc ttctcaccga cgatgcgctg gtctctgagc
    10261 aactccactt cggtgatccc cggcgcactg gtgttgacca ccgtaaaggt caccggcccg
    10321 gcgggcacgt gggtggtgtc cagcgcacag ccgtcttttc cgccgttgtt ggccatgctg
    10381 acgttgacca cgctgctgcc gccatgctgc ccaggtttcg aaggactcga ggtgccgctc
    10441 gaatggccgc acgcggtcac ggacaacagc gcggccccga ccaacgccgg tgcggtcagc
    10501 cacaattcac gaggccggca ccttacccgg gtgttgcgac tcactatcaa tcgtgttccc
    10561 ttcgtgttcg gttcgcgcgc ggtcgccgcg caccgagaat agggcgacca aggcgctgac
    10621 tgcgcaggct agcgcaaaac cggccagtac caacggaagc cagactttcc acagctgccc
    10681 ctctgcgcga ttgcggtcgg ttgcggcgat ctgcgccgcg gtcgacgcgt cttcggcgcc
    10741 ggccgtggac caatcggtgg gcagcccgcc gatgctgacc gtctttgccg cggtcagccc
    10801 gccgccggtc aggacagccg tgcggttgct gattgcgcgg gcgctcacca cgaagtctcc
    10861 ttgggcgagc accgtgtaca cggtgctggt cgccagctgc gcctggaagg gacccggggt
    10921 ccgggccgcg gagacaccca ccggcaacct gtcccccgtc atgcgcaata gctgctccag
    10981 ggttagagcc gtcgcgccgt caccggcggg ggccccgttc gaggtcacca cgccctgcca
    11041 cacccgcacc cacacgccgt ccaccgtttg atcgcccacc gcggcgaggg ggatgctgcg
    11101 gacgttcgcc gcgccctcgg ggacgatcgc cagcatgctt ccgtgcggcc cgggggtcag
    11161 cgacaccgtg gaggtgtggc cgtcctgttc ggtcaccgtg cgtgtatgca ctccggatgc
    11221 gtcgccaccg gagggcaccg cgaaggccaa taccgccgcg acgatgagca gcgcggccga
    11281 ggtggcggcc agcagccggc accggattcg cggcgctgcg gcaagtccag cgggccacag
    11341 aaagacgatg agcacgggca ccgcgtagag gagccagccc agcacctcga tgaggcgggg
    11401 gtcggcgggt atcccgaaca ccccggtgac aagtgcgccc agcacggacc ggctgggtat
    11461 ccaggcggag aagtcgagca cctgctgttg gccgatgctg atccagcccg cctcgtgcgc
    11521 cgcgcgcagc gcacccgtca ccaatccggc cgcgatgagc accaaaaaca ccccggtgac
    11581 gcgaaagaag cggcccaggt tgagtttcaa gccgccgaag tacatgccgg cgcccagcgc
    11641 gatcgacacc gcgatgccgg ccacgccacc cagcaacgcc aaccaccggt tcccgtacga
    11701 agtctccgcg gccgccagca agaacaccga cgtctcaaag ccctccttga gaaccgcaag
    11761 aaaggccatg gcggccaatg cgagcgcgcc accctggttg agggcctttt gggcatcgcg
    11821 ctcgagctcg cccttgagcc gtgcggcgtt gcgattcatc cagatgatca tcgaggtcac
    11881 gaacacgacg gcgatcgcgt tgatgaccgt ctccatcatc tcttgcgagg cttgcggcag
    11941 ggtcgccgag aggagatcca gaccaacgcc gaccgacgcg ctgatcagca cggccgcggc
    12001 gacgccggca aacatcggac gggtgctttg gctgtttcgc ttgaggaagg caccgatgat
    12061 gctgacgatg agcgtcgcct cgaggccttc acgcaggccg atgaggaacg tgccgataaa
    12121 aacgccgaat acgcc
//
LOCUS       DASSGQ010000085        12211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_705082,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000085 DASSGQ010000000
VERSION     DASSGQ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12211
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705082"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="VFI55_03880"
     CDS             complement(<1..1526)
                     /locus_tag="VFI55_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7073208.1"
                     /translation="MRSWWGWGTVEDALSEQETRDLVARVATLLPGHDLTDHQAPDPN
                     ALGLATPRITPPSSLADLCSIDPVDRAGHAHGKAFRDVARNLGGRLDHVPDLIVRPRR
                     ERDVVDVLDWCARQGISVIPYGGGSSVVGGVEPRFDDPVLTVDTTAMSAVLEIDRTSR
                     AARIQAGALGPVIENQLRPYELTLRHFPQSFGFSSLGGWLATRAGGHFATLYTHIDDL
                     TESLRVVTPVGVSESRRLPGSGAGPSPDGLFLGSEGTLGIITEAWMRLQDRPKWQVTA
                     SLTFDDWVAALTATRTVAQAGLYPSNCRLLDPAEAFLNAGAAVKGGLLVLAFESADHP
                     VGPWLDRAVEIVAEHGGTLTARRSRETGTQTTKNDASDASGNWRSAFLRMPYQRDALA
                     RRGVIAETFETACTWDGFDGLHAAITEAARKAIERVCGTGVVTCRFTHVYPDGPAPYY
                     GIYAGGRWGSLDTQWDEIKAAVSEAIITNGGSITHHHAVGRDHRPWYDRQRPDPFAAA
                     L"
     gene            complement(1537..1740)
                     /locus_tag="VFI55_03885"
     CDS             complement(1537..1740)
                     /locus_tag="VFI55_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073209.1"
                     /translation="MARGEIDPIRAKSALAVIKQNPGIVLFAVSPALVALGVVWWLAG
                     VGWAVAAAILLLIAGTAAIVLRR"
     gene            complement(1842..2171)
                     /locus_tag="VFI55_03890"
     CDS             complement(1842..2171)
                     /locus_tag="VFI55_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_007411850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073210.1"
                     /translation="MWELGVLLLLVAVLGVFLAQRFIPRGPRGELVSGTLLVTGVSPR
                     PDASGQQYVTIAGVINGPTVNEYAVYQRLVVDVDHWPSMGQLFPVVYSPKNPDNWRLA
                     PPGPPVG"
     gene            2260..3048
                     /locus_tag="VFI55_03895"
     CDS             2260..3048
                     /locus_tag="VFI55_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET7073211.1"
                     /translation="MSPDDLTLALALADRADGLTSARFGALDLRVDTKPDLTPVTDAD
                     RAVETELRYVLGRERTEDSVVGEEFGGTTTFSGRQWIIDPIDGTKNFVRGVPVWASLI
                     ALLEDGVPSVGVVSAPALQRRWWAASGQGAFAAVDGGPPRRLSVSSVAQLGSASLSFS
                     SLSGWAQRGVRDRFIELTDAVWRVRAYGDFLSYCLVAEGAVDIAAEPEVSVWDLAALD
                     VLVREAGGSLTGLDGVAGPHRGSAVATNGLLHQQVLAHLRTENV"
     gene            3133..4554
                     /locus_tag="VFI55_03900"
     CDS             3133..4554
                     /locus_tag="VFI55_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7073212.1"
                     /translation="MTDTHSGTKQTSKNSRSPIKRNGRKTGVGLQPHRRTGIDITLAL
                     LTPIVGQEFLDKYHLRDPLNRGLRYGTKTMFSSAGAASRQFKRVQNLRGGPTRLKSSG
                     KDYFDLTPDDEQKMIVETLDEFAEEVLRPAAHDADEAATYPPELISRAAELGITAINI
                     PEDFDGIAAHRSSVTNVLVAEALAYGDMGLALPILAPGGVAAALTHWGSADQQATYLT
                     EFAGDNVPQACVAIAEPQPLFDPTRLKTTAVRTPSGYRLDGVKSLVPAAADAELFVVG
                     AQLNGKPALFIVESSANGLTVKADPSMGIRAAALGQVELSGVSVPLSARLGPEDAAEA
                     DFDTAYSEAIALSRLGWAALAVGTSHAVLDYVIPYVKQREAFGEPVARRQAVAFMCAN
                     IAIELDGLRLITWRGAARAEQGLPFAREAALAKRLGADKGMQIGLDGVQLLGGHGYTK
                     EHPVERWYRDLRAIGVAEGVVVI"
     gene            4592..5800
                     /locus_tag="VFI55_03905"
     CDS             4592..5800
                     /locus_tag="VFI55_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7073213.1"
                     /translation="MAINLELPRKMQAVIDKAHQGAAEMMRPIARKYDLKEHAYPVEL
                     DTLSNLFSGAAESNALGLAGAEAFRGGEDKDENRNGANMAAGLQTLEASWGDAAMMLS
                     MPYQGLGNAAISGVASDEQLSRLGRVWAAMAITEPGFGSDSAAVSTTAKLDGDEYVIN
                     GEKIFVTAGSRATHIVVWATLDKSLGRPAIKSFIVPREHPGVTVERLENKLGIKGSDT
                     AVIRFDNARIPKDNLLGNPEIESGKGFSGVMETFDNTRPVVAAMAIGIARATLEELRK
                     ILTEAGIEISYDKPSHAQSAAAAEFLRMEADFEASYLLTVRAAWQADNKIPNSKEASM
                     AKAKAGRVGSDITLKAVELAGTAGYSEQTLLEKWARDSKIMDIFEGTQQIQLLVVARR
                     LLGLSSAELK"
     gene            complement(5810..6505)
                     /locus_tag="VFI55_03910"
     CDS             complement(5810..6505)
                     /locus_tag="VFI55_03910"
                     /inference="COORDINATES: protein motif:HMM:NF037007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7073214.1"
                     /translation="MTAQTKPERARGRPRASDSMPIEEILDKALTMFATVGYDGMSLR
                     TLNRELGVSHNLIHQRFGTKEGQRFGTKEGQRFGTKEGLWRAAVDYGFGRLMRHMQGL
                     FDPTISEPLEQLRHAVHRFVMFSADHPELVALMNIEGRQDTDRLAYIYDTYIEPMLTD
                     VARLLVHLADNGVIRPIPLRTFHFLLAHGATAPQALVPLATKFDATSPFEPAAIEQHA
                     QLVSTLLVEGLRL"
     gene            6609..8270
                     /locus_tag="VFI55_03915"
     CDS             6609..8270
                     /locus_tag="VFI55_03915"
                     /inference="COORDINATES: protein motif:HMM:NF004780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7073215.1"
                     /translation="MTRTLHVPVLIAGGGPTGLVLAAELSRHGVACVLAERNEHTTLF
                     PKMDITNGASMELLRRLGMDAELRAVGVGAQHPFDVIFAAGLDGPIHGRWRLPSVDEQ
                     RGIIAATADGSVPGQPWQRCSQAIFEAIMMDRARRDPRVDVRQGWRLQRCTQAGDVAA
                     AELSDSDGNTVTVHADYVVGCDGASSRVRTELGIEMDGLKDFTTFALVHFRSSDLTNL
                     HALGQFWHLYTSGGVVLIAQNEIDTWTLHRDLGAQVDDPDPIGDPREFVARALGRPIV
                     IDDVLASSVWRPNAMLAGSYGRDRILLAGDAVHTMIPTGGYGMNTGLGDAVNLGWKLA
                     ATIQGWGGPALLASYEIERRPIADRNRNACVENAMVILQYRDMLDPQLFDQDNEAGQA
                     HRQRVAEFLATNNAENLSLGVELDVRYDGSPVVVADGSATPPWDRRTFVPTVRPGHRA
                     PNIALGKHDTLFDQFGPGFTLVDALDDPSQSALLLGEAARVGLPIRHLTLTDPALAAL
                     YRHRLVLVRPDLHVAWSGTDASCAADIIASVRGMHPATAPATVNT"
     gene            8313..8687
                     /locus_tag="VFI55_03920"
     CDS             8313..8687
                     /locus_tag="VFI55_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7073216.1"
                     /translation="MNALLWTLQIVLALVFAGAGTVKLVQPRDQLAKRLGGWVYDFPA
                     PLLKPLGLAELLGAIGLIVPPLVHVAPILTPVAAAGLVVVMIGALVTHARRREFPNVA
                     VTVLLAAMAAVVAWGRFGPYSF"
     gene            complement(8715..9572)
                     /locus_tag="VFI55_03925"
     CDS             complement(8715..9572)
                     /locus_tag="VFI55_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009955101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET7073217.1"
                     /translation="MDSFPLGGYSVGRVGFGAMQLPGPGVFGPPRDREQALAVLRRAV
                     ELGVDHIDTAQFYGPDVANELIREALYPYPANLALVSKVGGRRDDAGAWLPLAEPADL
                     RRDIEANLSTLGIDQLAAVNLRLFESKAPDRLFDDQLSVMIDARDDGLIGGIGLSAIT
                     REHLLHALERTEIACVQNSFNLVDRSSAGVLEECTARGIAFVPFFPLGAAFLQPNPVL
                     THPVVAEIAQQLGRTPAQVALAWTLSVAPNVLLIPGTSSVRHLEENLAVADIALDEDS
                     RERLSAVAG"
     gene            9596..10603
                     /locus_tag="VFI55_03930"
     CDS             9596..10603
                     /locus_tag="VFI55_03930"
                     /EC_number="1.3.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009955102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acryloyl-CoA reductase"
                     /protein_id="HET7073218.1"
                     /translation="MDTFQALVARQDGDRITASVETLSPSDLPPGEVTIRVLYSSVNF
                     KDALAVTPGGGVVRSYPLVPGIDLTGEVVESQSPDFAVGDKVLAHGYQIGTGHHGGYA
                     EYVRLPADQLVVLGTLSPHDGAAIGTAGFTAAMSVQALIDWGITPDAGPIVVTGATGG
                     VGSVSVDLLAAAGYRVVASTGKSQQDQVADRLKALGAAEVIGRLPADPDAKPRPLGKT
                     RWAGAVDCVGGATLADVLSTIDYGGAVAASGLTGGAALHTTVMPFILRGVALLGMDSV
                     LMPIGPRRKLWARLGDSLRPRHLDDVTTDVDVKDVVGVLDQVRAGAFSGRAVVRVAGG
                     F"
     gene            10643..11614
                     /locus_tag="VFI55_03935"
     CDS             10643..11614
                     /locus_tag="VFI55_03935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HET7073219.1"
                     /translation="MRAVQVTRLDGPDGIVVTEVDEPEGEGVVVEVHAAGAAFPDALL
                     TRGLYQYRPEPPFVLGAEIAGVVRSAPDGSGLRAGDRVVGLTMLIGGMAEVAVLQPDR
                     VFKLPDNVTFEAAAGLLFNDLTVYFTLSVRGRLQQGETVLVHGAAGGIGTSALRLAPV
                     LGASRTIAVVSTEEKGEIATAAGATDVVLAEGFKDAVKELTSDRGVDIVVDPVGGDRF
                     TDSLRSLAPGGRILVVGFTGGEIPSVKVNRLLLNNIDVVGVGWGAWALTHPGALAQQW
                     AALKSLLSSGRLPPPKPVVYPLEEAAAAVASLENRTAKGKVVLRVRS"
     gene            11877..>12211
                     /locus_tag="VFI55_03940"
     CDS             11877..>12211
                     /locus_tag="VFI55_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET7073220.1"
                     /translation="MNGALDGIRVLELGTLIAGPFAGRLLGDMGADVIKVEPPGAPDP
                     LRTWGQAELDGHHVFWTVHARNKRAITLDLRRPRGRELFLELVEKSDIVVENFRPGTL
                     EGWSLGYDVL"
BASE COUNT         2045 a         4121 c         4128 g         1917 t
ORIGIN      
        1 agcgccgccg cgaacggatc cggccgctgt cggtcgtacc agggccggtg atcgcggccg
       61 accgcgtggt gatgggtgat gctgccgccg ttggtgatga tggcctccga gaccgcggcc
      121 ttgatctcgt cccactgcgt gtccagcgag ccccagcggc cgccggcata gatgccgtaa
      181 tacggggccg ggccgtcggg atacacgtga gtgaaccgac aagtcaccac gccggtgccg
      241 cagactcgtt cgatcgcctt ccgggccgcc tcggttatcg cggcatgcag cccatcgaat
      301 ccgtcccagg tgcaagcggt ttcgaatgtc tcggcgatga cgccgcgccg ggccagggcg
      361 tcgcgctggt agggcatccg caagaatgcc gagcgccagt tcccggaagc atctgacgcg
      421 tcgttttttg ttgtctgggt tccggtttcg cggctacgcc gtgcggtcag cgtgccaccg
      481 tgttcggcga cgatctccac ggcccggtca agccacgggc caacgggatg gtcggccgat
      541 tcgaaagcca gcaccaacag ccctcctttg accgcggcgc cggcgttcag gaacgcctcc
      601 gccgggtcca gcagccggca gttcgacggg tagagcccgg cctgcgcgac cgtccgggtc
      661 gcggtcagcg cggcgaccca gtcgtcgaat gtcaacgacg cggtgacctg ccacttcggg
      721 cggtcttgta accgcatcca ggcctcggtg atgatgccga gcgtgccctc ggagccgagg
      781 aacaacccgt ccggggatgg tccggcgccg gatccgggca gccgccggga ctcgctcacc
      841 ccgaccgggg tgacgacccg cagcgattcg gtcaggtcgt cgatgtgggt gtacagcgtg
      901 gcgaagtgcc caccggcgcg ggtggccagc cagccgccga ggctggagaa accgaacgac
      961 tgcgggaaat gccgcagcgt caattcgtat ggacgtagct ggttttcgat caccggcccg
     1021 agtgcgccgg cctggattcg cgccgcgcga ctggtccggt cgatctcgag gaccgcgctc
     1081 atggcggtgg tgtccaccgt gaggaccggg tcgtcgaacc gcggctcgac gccgccgacc
     1141 accgagctgc caccgccgta cgggatgacc gagattcctt ggcgcgcaca ccaatcgagc
     1201 acgtcgacaa cgtcccgctc acggcgcggt cgcacgatca ggtcaggcac gtggtcgagt
     1261 cggcctccca gattgcgcgc aacgtcgcga aaagccttgc cgtgcgcgtg gccggcccgg
     1321 tcgacggggt cgatcgaaca caggtcggcc agcgacgacg gcggggtaat ccgcggcgtc
     1381 gccaacccaa gcgcgttcgg gtccggcgcc tggtgatcgg tcaggtcgtg cccgggcagg
     1441 agcgtcgcca cacgtgcgac cagatcacgt gtttcctgct ccgagagcgc gtcttcgacg
     1501 gtgccccaac cccaccatga acgcatgctg acggcgtcag cgccgcaaga cgatggcggc
     1561 cgtaccggcg atcagtagca ggattgccgc ggcaacggcc cagccgacgc cggcaagcca
     1621 ccagacgacg cccaacgcca cgagcgccgg cgacaccgcg aagagcacta ttccggggtt
     1681 ctgcttgatc accgcaagtg cactcttcgc ccggatcgga tcgatttcac cacgcgccat
     1741 gctatttaga atgccagttc aggacgcgta gcgaaatcca ctggcggcgt cggtgcaatc
     1801 ccggcgtcgg tgcagtctgt tctgggtgaa cgccgccggc gtcagcccac cggcggcccg
     1861 ggcggtgcga gcctccagtt gtcgggattc ttcggcgaat acaccaccgg gaacagttgg
     1921 cccatgctcg gccagtgatc gacgtcgacc accaagcgct gatacaccgc gtattcgttg
     1981 accgtgggtc cgttgatcac gccggcgatg gtcacgtact gctggccgct cgcgtccggt
     2041 cgcgggctga ccccggtcac cagcaacgtg ccgctcacca gctcaccgcg gggaccgcgc
     2101 ggaatgaagc gctgcgcgag gaacaccccc aggacggcga cgaggaggag cagcacacct
     2161 aattcccaca cccggccatg gtagggccgt gataggactg ctggcatgag tcgcaccggc
     2221 gacgatgcag aagcggagcg atgaagagga gcggtgcgca tgagtccgga cgacctgacg
     2281 ctggcgctcg cgctggccga ccgcgcggat ggactgactt ccgcccgttt cggcgcgctg
     2341 gacctgcggg tcgacaccaa gccggacctg acgccggtga ccgacgccga tcgcgcggtg
     2401 gaaaccgagc tgcgatacgt gctcggacgc gaacgcaccg aggacagcgt cgtcggcgag
     2461 gagttcggcg gaaccaccac attcagcggg cggcagtgga tcatcgaccc gatcgacggc
     2521 accaagaact tcgtgcgcgg ggtgccggtg tgggccagtt tgatcgcgtt actcgaagac
     2581 ggcgtcccct cggtcggcgt cgtgagcgcg ccggcgctgc aacgccggtg gtgggcggcg
     2641 agcggccagg gggcgttcgc ggccgtcgac ggcgggccgc cgcgccggct gtccgtctcg
     2701 tcggtcgcac agctgggttc ggccagcctg tcgttctcca gcctgtccgg atgggcgcag
     2761 cgcggcgtgc gcgaccggtt catcgagctg accgacgcgg tgtggcgggt ccgcgcctac
     2821 ggcgactttc tgtcttattg cctcgtcgcg gagggggcgg tcgacatcgc cgccgaaccc
     2881 gaggtgtcgg tgtgggacct ggcggcgctc gatgtgttgg tgcgtgaggc gggcgggtcg
     2941 ctgaccggcc tggacggcgt agcgggcccg caccgcggca gtgccgtggc gacaaacggc
     3001 ctgctgcatc agcaggtgct cgcccacctg cgcaccgaaa acgtgtgagt tactcaacac
     3061 ggcagctcta gctaccttac tccggagtaa gataggcttg tctgcatcgc cccaacgacc
     3121 gaggctgctg aaatgaccga cactcattcg ggtacaaaac agacttcgaa aaactcccgc
     3181 tcacccatca agcggaacgg ccgaaaaacc ggcgtcgggc tgcaaccgca caggcggacc
     3241 gggatcgata tcaccctggc gcttctcacc ccgatcgtcg ggcaggaatt tctggataaa
     3301 taccatctgc gcgatccact caatcgcggc ctgcgctacg gcacgaagac gatgttctcc
     3361 agcgccggcg ccgcttcccg ccagttcaag cgggtccaga atctgcgcgg cggaccgacg
     3421 cggctcaagt ccagcggcaa ggactacttc gacctgacgc ccgacgacga gcagaagatg
     3481 atcgtcgaga ccctcgacga attcgccgag gaggtcctgc gacccgcggc acatgatgcc
     3541 gacgaggcgg cgacgtatcc gcccgagctg atctcgaggg ccgcggagtt gggcatcacc
     3601 gccatcaaca tccccgagga tttcgacggc atcgccgcac accggtccag cgtgaccaac
     3661 gtgctggtgg ccgaggcgct ggcatacggc gatatgggtc tggcgttgcc gatcctggcc
     3721 cccggaggcg tggcggccgc gctgacccac tggggcagcg ccgaccagca ggccacctac
     3781 ctcaccgagt tcgccggcga caacgtcccg caggcctgcg tggccatcgc cgaaccgcag
     3841 ccgctgttcg accccacccg gctgaagacg accgcggtgc gcacccccag cgggtaccgg
     3901 ctcgacgggg tgaagtcgct ggtacccgcc gcggccgacg ccgagttgtt cgtcgtcggg
     3961 gcgcagctga acggcaaacc ggcactgttc atcgtcgagt cgtccgccaa cggccttacc
     4021 gtcaaggcgg atccgagcat gggaattcgc gcggctgccc tgggccaggt cgaactctcc
     4081 ggagtgtcgg tgccgctgag tgcccggctg ggtccggaag acgccgccga ggccgacttc
     4141 gacaccgcct actccgaggc gatcgcgctg tcccggctgg gctgggcggc gctggccgtc
     4201 gggacctcgc acgcggtgct cgattacgtc atcccgtacg tgaaacagcg cgaagctttc
     4261 ggcgagccgg tcgctcgtcg tcaagcggtc gcgttcatgt gcgccaacat cgccatcgag
     4321 ttggacgggc tgcggctgat cacctggcgc ggcgccgccc gggccgaaca gggcctgcct
     4381 tttgcccgcg aagcggcact ggccaagcgg ctcggcgccg acaagggcat gcagatcggc
     4441 ctggacggcg tgcaactgct cggcggccac ggctacacga aagaacatcc ggtcgagcgc
     4501 tggtaccgcg acctgcgggc catcggcgtg gccgagggcg tagtggtcat ctaggtagtc
     4561 atccaggtcc tcatcgagcg aaagatcaat catggcaatc aatctggaac tgccccgcaa
     4621 gatgcaggcg gtgatcgaca aggcacacca gggcgccgcg gagatgatgc ggccgatcgc
     4681 gcgcaaatac gacctcaagg aacacgccta cccggtcgag ctcgacacgc tgtccaacct
     4741 gttctccggg gccgccgagt ccaatgccct gggactcgcc ggggccgagg cgtttcgcgg
     4801 cggcgaagac aaggacgaaa accgcaacgg cgccaacatg gcagcggggc tgcagaccct
     4861 cgaggccagc tggggcgacg ccgcgatgat gctgtccatg ccctaccagg gcctgggcaa
     4921 cgcggcaatc tccggtgtgg ccagcgacga gcagttgagc cggttgggca gggtgtgggc
     4981 ggcgatggcc atcaccgagc cgggctttgg ctccgactcg gcggcggtgt ccaccaccgc
     5041 caagctcgac ggcgacgagt acgtgatcaa cggcgaaaag atcttcgtca ctgcgggttc
     5101 ccgggccact catatcgtgg tgtgggcgac gctggacaag tcgctgggtc gcccggcgat
     5161 caagtcgttc atcgtgccgc gcgaacatcc cggcgtcacc gtcgagcggc tcgagaacaa
     5221 gctcggcatc aagggctccg ataccgccgt catccggttc gacaacgcgc ggatcccgaa
     5281 ggacaacctg ctggggaacc ccgaaatcga gtcgggcaag gggttttccg gggtgatgga
     5341 gaccttcgac aacacccggc cggtcgtggc cgccatggca atcggcattg ccagggccac
     5401 actggaagag ctgcgcaaga tcctgaccga ggccgggatc gagatctcct atgacaaacc
     5461 gtcgcacgcc caaagcgccg ccgcggccga attcttgcgg atggaggccg atttcgaggc
     5521 cagctacctg ctgaccgtgc gcgcggcgtg gcaggcggac aacaagatcc ccaactccaa
     5581 ggaagcctcg atggccaagg ccaaggccgg ccgggtgggc agcgacatca ccctgaaggc
     5641 cgtcgaattg gccggcacgg caggctattc cgagcagacg ctgctggaga agtgggcacg
     5701 tgactcgaag atcatggaca tcttcgaggg cacccagcag atccagctac tggtggtcgc
     5761 gcgccgactg ctgggcctgt cctcggccga gctcaagtag ttcatcgagt cacagccgca
     5821 gtccctccac caggagggtg gaaaccagct gcgcgtgctg ctcgatcgcg gcgggctcga
     5881 atggcgaagt cgcgtcgaac ttcgtcgcga gcggcaccag cgcctgcggc gcggtggcgc
     5941 catgggccag cagaaagtgg aaggtccgca acggaattgg gcgtatcacc ccgttgtcgg
     6001 ccaggtggac gagcagccgg gcgacgtcgg tgagcatcgg ctcgatgtag gtgtcgtaga
     6061 tgtaggccag ccggtcggtg tcctggcggc cctcgatgtt catcaacgcg accagctcgg
     6121 gatgatcggc cgagaacatc acgaatcggt gcacggcatg acgcagctgc tcgagcggtt
     6181 cgctgatggt cgggtcgaag aggccctgca tgtgccgcat caggcgaccg aagccgtagt
     6241 cgaccgccgc ccgccacaat ccctccttgg tgccaaagcg ctgaccctct ttggtgccaa
     6301 agcgctgacc ctccttggtg ccaaagcgct gatggatcag gttgtgactg acaccgagct
     6361 cccggttgag ggttcgtagc gacatcccgt cgtatccgac cgtggcgaac atggtcagcg
     6421 ccttgtccag gatctcctcg atcggcatgg aatccgacgc cctgggacgc ccacgcgctc
     6481 gctccggctt ggtttgggcg gtcacgccaa ccagcatgcc atatcacttg cccatttata
     6541 tattgacagt cagcaattta agttctagct tctgacttac cgcaccgaca gccgcaggag
     6601 cgaccaccat gacgagaacg ctgcatgtac ccgtactcat cgccgggggc ggcccgaccg
     6661 gattggtgct ggcagctgag ttgtctcggc acggcgtcgc ctgcgtgctg gccgaacgca
     6721 acgagcacac cacgctgttt cccaagatgg acatcactaa cggcgcgagc atggaactgc
     6781 tgcgccgcct gggcatggac gccgagctgc gggcggtcgg ggtgggcgcc cagcatccct
     6841 tcgacgtcat cttcgccgcc ggtctggacg ggcccatcca cggacgctgg cggctgccgt
     6901 cggttgacga gcagcgcggc atcatcgcgg ccaccgccga tggcagcgtg cccggccaac
     6961 cgtggcagcg ctgctcccag gcaatcttcg aggcgataat gatggaccgc gcccgccgcg
     7021 acccgcgagt cgacgtccgg caaggctggc ggctgcaacg ctgcacccag gccggcgacg
     7081 tggcggccgc cgaactgtcc gacagcgacg gcaataccgt caccgtccac gccgattacg
     7141 tcgtcggctg cgacggcgcc tccagtcggg ttcgcaccga actgggcatc gaaatggacg
     7201 gcctgaagga tttcaccacc ttcgccctgg tccatttccg ctcctcggat ttgacgaacc
     7261 tgcatgccct gggccagttc tggcatctgt acacctccgg cggtgtggtg ctgatcgccc
     7321 aaaacgagat cgacacctgg acgttgcatc gcgacctggg tgctcaggtc gacgatcccg
     7381 atccgatcgg tgatccccga gagttcgtcg ctcgcgccct gggtcgtccg atcgtcatcg
     7441 acgacgtcct cgcgtccagc gtgtggcgac ccaacgccat gctggccggc tcgtacggcc
     7501 gtgaccggat cctgctggcc ggcgacgcgg tgcacacgat gatccccacc ggtggatacg
     7561 gcatgaacac cggactcggc gacgcggtca acctcggctg gaaactcgcc gcgacgatcc
     7621 agggatgggg cggcccggcg ctgctggcca gctacgagat cgagcgacgc ccgatcgccg
     7681 accgcaatcg caacgcctgt gtggaaaacg cgatggtcat tctgcaatac cgcgacatgc
     7741 tcgacccgca actcttcgac caagacaacg aggccggaca agcgcaccgt caacgcgtgg
     7801 cggagttcct cgccaccaac aacgccgaaa acctcagcct cggtgtcgaa ctcgatgtgc
     7861 gctacgacgg ctcaccggtg gtggtcgccg acggcagcgc caccccaccg tgggatcgcc
     7921 gcaccttcgt ccccaccgtg cggcccggcc accgcgcccc caacatcgcg ctggggaagc
     7981 acgacacgct gttcgatcag ttcggccccg gtttcaccct ggttgatgcg ctggacgacc
     8041 ccagccaatc tgctctgctg ctcggcgagg ccgcccgggt cggcctgccg atccggcatc
     8101 tgacactcac cgaccccgcc ctggcggcgc tctaccggca ccggctggtc ctggtccgcc
     8161 cggatctgca cgtcgcgtgg agtggcaccg acgcctcctg cgccgccgac atcatcgcca
     8221 gcgtccgcgg catgcacccg gccacagcgc cggccacggt caacacctaa gcgcggcaac
     8281 ctgattcgca caccacacag aaacgaacaa cgatgaatgc cctgctatgg acactgcaga
     8341 tcgtgctggc cctcgtcttc gccggggccg gcaccgtcaa actcgttcag ccccgggacc
     8401 aactcgccaa gcgtcttgga ggctgggttt acgactttcc ggctcccctg ctcaaaccgc
     8461 tcggtctcgc cgaattgctg ggcgccatcg gactgatcgt gccgccgctg gtccacgtcg
     8521 cgccgatcct gacgccggtc gcagccgccg ggctagtcgt cgtcatgatc ggcgcgctgg
     8581 tcacccacgc ccgccgacgc gaattcccca acgtcgccgt caccgtcttg ctggcagcaa
     8641 tggccgcagt cgtcgcctgg ggacgattcg ggccctactc cttctgatca ggcaccgcca
     8701 gatgcgcgcc ggcattaccc ggcgaccgcg ctcagccgct cgcggctgtc ctcgtccaac
     8761 gcgatgtcgg cgacggccag gttctcctcc aggtgccgca ccgacgaggt gcccgggatc
     8821 agcagcacgt tgggcgccac gctgagtgtc cacgccagcg cgacctgcgc cggcgtgcga
     8881 ccgagttgct gagcgatctc ggccaccacc gggtgggtca gcaccgggtt cggctgcagg
     8941 aaggccgctc ccagcgggaa gaacgggacg aacgcgatcc cccgcgcggt gcattcctcc
     9001 agcaccccag ccgatgaccg gtcgacgagg ttgaaagagt tttgcacaca ggcgatttcg
     9061 gtgcgttcca acgcgtgcag caggtgctcg cgggtgattg cgctcaggcc gattccgccg
     9121 atcagtccgt cgtcacgggc gtcgatcatg accgagagct gatcgtcgaa aagccgatcc
     9181 ggcgctttgc tctcgaacag gcgcagattg accgcggcca gctggtcgat accgagggtg
     9241 ctcaggttgg cttcgatatc gcggcgcaaa tcggcgggct cggcgagcgg cagccaggcg
     9301 ccggcgtcgt cgcgccgccc accgaccttg ctcaccagcg ccaggttcgc cgggtacgga
     9361 tacagcgcct cccggatcag ctcgttggcg acgtcgggac cgtagaactg ggcggtgtcg
     9421 atgtggtcga caccgagttc gaccgcccgc cgcagtaccg ccagggcctg ttcgcggtcg
     9481 cggggcggcc cgaacacccc ggggccgggc agttgcatgg cgccgaaccc gacgcggcca
     9541 accgaatagc caccgagtgg aaaggaatcc atgcggtgag attagcgtcg tcgtcatgga
     9601 cacgtttcaa gcgcttgtcg cgcgtcaaga tggtgaccgg ataaccgcgt cggtcgagac
     9661 actgagcccg tcggacctgc cgcccggtga ggtgacgatc cgggtgctgt attcgagcgt
     9721 gaacttcaag gacgcgctcg cggtgacgcc cggcggcggc gtggtgcgca gctatccgct
     9781 ggttccgggc atcgacctga ccggcgaggt tgtcgagtcg cagtcacccg acttcgccgt
     9841 cggcgacaag gtgctcgcgc acggatacca gatcggcacc ggccaccacg gcggctatgc
     9901 cgaatacgtc cggctgccgg ccgaccagct ggtggtgctc ggcacattga gtccgcacga
     9961 cggcgcggcg atcggcaccg ccggcttcac cgccgcaatg agcgtgcagg cgctgatcga
    10021 ctggggcatc acgcccgacg ccggacccat cgtggtcacc ggcgccaccg gcggcgtcgg
    10081 ctccgtcagc gtggacctgc tggcggccgc cgggtaccgg gtggtggcct cgaccggcaa
    10141 atcccagcaa gaccaagtcg ctgaccgcct gaaagccctt ggcgccgcgg aggttatcgg
    10201 ccggctgccc gccgaccccg acgccaagcc gcgaccgctc ggcaagacgc ggtgggcggg
    10261 cgcggtggac tgtgtgggcg gcgcaaccct ggccgacgtt ctcagcacga tcgattacgg
    10321 cggcgcggtg gctgccagcg gcctcaccgg cggcgccgcc ctgcacacca ccgtgatgcc
    10381 cttcatcctg cggggcgtgg cgctgctggg catggattcg gtgctcatgc cgatcggccc
    10441 acgccggaag ttgtgggcgc ggctgggcga ttcgttgcgg ccgcgtcacc tcgacgacgt
    10501 caccaccgac gtcgacgtca aagacgtcgt cggcgttctt gatcaggtgc gggccggcgc
    10561 attctccggc cgggcggtgg tgcgggtcgc cggcggattc tgagttacgg ggcgtcagta
    10621 ggctaggcac gagggaggca gcatgcgcgc ggtacaggtg actcggctgg acgggccgga
    10681 cggcatcgtg gtgaccgagg tcgatgagcc cgaaggcgag ggcgtcgtcg tcgaggtgca
    10741 cgccgccggc gcggcgttcc cggacgcgtt gctgacccgg ggcctctacc agtaccggcc
    10801 ggaaccgccg ttcgtgctcg gggcggagat cgccggggtg gtgcggtcgg cgccggacgg
    10861 ctcggggcta cgtgccggtg acagagtcgt cggtctgacc atgctcatcg gcgggatggc
    10921 cgaagtcgcg gtcctgcaac ccgaccgagt gttcaagctg ccggacaacg tgaccttcga
    10981 ggcggccgcg gggctgctgt tcaacgacct gaccgtgtac ttcacgctga gtgtccgcgg
    11041 ccggttgcag cagggcgaaa cggtgctggt gcacggggca gccggcggga ttggaacctc
    11101 cgcgctgcgg ctggcgcccg tgctcggcgc gtcccgcacc atcgcggtgg tcagcaccga
    11161 ggagaagggc gagatcgcca cggcggccgg cgccaccgac gtggtgttgg ccgagggttt
    11221 caaggatgcg gtgaaggagt tgaccagcga ccgcggcgtc gacatcgtcg tcgacccggt
    11281 cggcggcgac cggttcacgg attcgctgcg ctcgctcgcc ccggggggac ggatcctggt
    11341 tgtcggcttc accgggggtg aaataccgtc cgtgaaggta aaccggctgc tgctcaacaa
    11401 cattgacgtg gtcggtgttg gctggggcgc gtgggccctc acgcaccccg gtgcgctcgc
    11461 ccaacagtgg gcggcgctca aatccctgct cagctcaggt cggttgcctc cccccaagcc
    11521 agtggtctac ccgctcgagg aggccgccgc cgcggtggcc tcgctggaga accggaccgc
    11581 caagggcaag gtcgtgctgc gggtacgcag ctgacggatt cggcggcgtc accgccggct
    11641 ttaggcccca cctggcacat gggcctcagc gcagaaggga tcgactatat gcccgcctcc
    11701 gagcgacaac caggccgtgg ccggttgtcg taccgaactt gtcgctccga ggcgggcaca
    11761 acgtgtgtcc cgtctccgag agacgccagt ggcgcatgtg actatgccgt cggcatagac
    11821 caccgcagcc cggagccacc gatggaatcc ttcgtccacg aattcgcgag cgaagagtga
    11881 acggtgcgct ggacggcatc cgggtgctcg agctgggcac cttgatcgcc ggaccgttcg
    11941 ccggccggct gctcggcgac atgggcgccg acgtgatcaa agtggaaccg cccggcgctc
    12001 cggatccgtt gcgcacctgg ggccaggccg aactcgacgg ccaccacgtc ttctggacgg
    12061 tgcatgcgcg caacaaacgg gcgatcacgc tcgacctgcg ccggccacgc ggccgggaat
    12121 tgttccttga actcgtcgag aagtccgaca tcgtggtgga gaacttccgt cccggcaccc
    12181 tggaggggtg gagcctgggc tacgacgtgc t
//
LOCUS       DASSGQ010000086        14017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_279750,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000086 DASSGQ010000000
VERSION     DASSGQ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14017
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279750"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..878)
                     /locus_tag="VFI55_03945"
     CDS             complement(201..878)
                     /locus_tag="VFI55_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7073221.1"
                     /translation="MTAVHDHYTGHVDPGTAARRTLPGATILKASVGPMDNNAYLVTC
                     SATGETLLIDAANDADVLIGLIRGYAPKLSLIVTSHQHFDHWQALEAVAAATGAPTAV
                     NEIDAGPLPVKPDRLLANGDTVQVGELSFDVIHLRGHTPGSIALALDGPATGGVTQLF
                     TGDCLFPGGLGRTTKPAEFDSLFDDVKARVFDRFGDQTVVYPGHGDDTTLGAERPHLG
                     EWRERRW"
     gene            999..3917
                     /gene="uvrA"
                     /locus_tag="VFI55_03950"
     CDS             999..3917
                     /gene="uvrA"
                     /locus_tag="VFI55_03950"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET7073222.1"
                     /translation="MADRLIVKGAREHNLRGVDLDLPRDSLIVFTGLSGSGKSSLAFD
                     TIFAEGQRRYVESLSAYARQFLGQMDKPDVDFIEGLSPAVSIDQKSTNRNPRSTVGTI
                     TEVYDYLRLLYARAGTPHCPICGERIARQTPQQIVDQVLAMEEGTRFLVLAPVVRTRK
                     GEFADLFDKLNAQGYSRVRVDGVVHPLTDPPKLKKQEKHDIEVVVDRLTVKASAKQRL
                     TDSVETALNLADGIVVLEFPDDRDEHDHAREQRFSEKLACPNGHPLAVDDLEPRSFSF
                     NSPYGACPECTGLGIRKEVDPDLVVPDPDRTLAEGAVAPWSNGHTAEYFTRMMAGLGE
                     ELGFDVDTPWAKLPAKARKAILEGSDHQVHVRYRNRYGRTRSYYADFEGVLAFLERKM
                     AQTESEQMKERYEGFMRDVPCPVCEGTRLKPEILAVTLAGESGGERDAKSIAEVCELS
                     ISDCADFLNALTLGQREQAIAGQVLKEIQSRLGFLLDVGLEYLSLSRAAATLSGGEAQ
                     RIRLATQIGSGLVGVLYVLDEPSIGLHQRDNRRLIETLTRLRDLGNTLIVVEHDEDTI
                     AHADWVVDIGPGAGEHGGRIVHSGTYKDLLVSKDSITGAYLSGKESIEIPAIRRPVDR
                     RRQLTVVGAREHNLRDIDVSFPLGVLTSVTGVSGSGKSTLVNDILAAVLANRLNGARQ
                     VPGRHTRVTGLDHLDKLVRVDQSPIGRTPRSNPATYTGVFDKIRTLFAATTEAKVRGY
                     QPGRFSFNVKGGRCEACTGDGTIKIEMNFLPDVYVPCEVCHGARYNRETLEVHYKGKT
                     ISEVLDMSIEEAAEFFEPISGIHRYLRTLVDVGLGYVRLGQPAPTLSGGEAQRVKLAA
                     ELQKRSTGRTVYILDEPTTGLHFDDIRKLLKVINGLVDKGNTVIVIEHNLDVIKTSDW
                     IVDMGPEGGAEGGTVVAQGTPEDIAAVPESYTGKFLAEVVGDGAASPQRSTRRRKASA
                     "
     gene            complement(3997..5172)
                     /locus_tag="VFI55_03955"
     CDS             complement(3997..5172)
                     /locus_tag="VFI55_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HET7073223.1"
                     /translation="MRRFTITERRNRLARRHFLARTGSRPIAEVAAALVGLHATDPAT
                     PYLSLWARTPEFAVADLEQELYQKRAVVRHLAMRRTLWLVNGDDLAMIQSAFSNRVAT
                     NERRRLAADVHKAGVAYDGDRWLELACTAVLRHLGDNEPASSTELRAALPELAGTYDP
                     APGKRWGGSVPLAPRVLTLLSARGDIVRGPNDGSWTTSRPRWATTADWLGRPFEVPPA
                     QAAQAELIRRWLRTFGPATAADIKWWFGITLTAARKTLADIGAVKVDLHGSDGYALPD
                     DLEPEPAVAPWDALLPGLDVTTMGWFERDWYLGEHRAQVFDRNGNAGPTAWWNGRVVG
                     GWYQDDSARVQLQLTEDPGGDGRRALQRRADGLTSWLDGVRISPRFPSPLSKAGSTG"
     gene            complement(5202..5450)
                     /locus_tag="VFI55_03960"
     CDS             complement(5202..5450)
                     /locus_tag="VFI55_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073224.1"
                     /translation="MPDESATPDTSEPGAGYDSAGVPTFESVREKIETRYATAQGAAE
                     LDAETAEGRSVEEQFEERRRAAAERLAQIRESMRPEER"
     gene            complement(5460..6011)
                     /locus_tag="VFI55_03965"
     CDS             complement(5460..6011)
                     /locus_tag="VFI55_03965"
                     /inference="COORDINATES: protein motif:HMM:NF042492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6653 family protein"
                     /protein_id="HET7073225.1"
                     /translation="MSGGDAQRVAQHVPAVGVFRARGDGRALPRRHPVTALKPHVARI
                     RRAIFARHCNPWSAWTRWASTPLILVPVWTRRWSHGLWIALWLAINPFVFGKPADERA
                     WSTRAMLGEELWISRRRGDGAMLVSVATSAAALCGVLAARRHRMIPALIASAVQMALT
                     LVYWEQMVRYFDRQPRAKDTPRQ"
     gene            complement(5909..7387)
                     /locus_tag="VFI55_03970"
     CDS             complement(5909..7387)
                     /locus_tag="VFI55_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET7073226.1"
                     /translation="MDHVTASIPPATAQLLASNFTSLMHGWIPATIQITTAIVLTLGI
                     GWRSRRWRMIWLPAAGLVGGGAVYATHWYIVDRSLSDEPAPTTLWVWVALTGMAAAVL
                     VLGWRHAEPWRRGATLLAVPMCLLCAALVLNLWVGYFPTVPSAWNALTYGPVPDQTDQ
                     ATVTAMVGKRARPPHGSVVPVVIPSDASHFKHRGELVYLPPAWFTSSPPPALPTVMMI
                     GGQFNTPADWTRAGNAVKTIDDFAATHDGNAPVLVFVDPGGAFDNDTECVNGVRGNAA
                     DHLTKDVVPYMISNFGVSRDRSNWGVVGWSMGGTCAVDLTVMHPDMFSAFVDIAGDFF
                     PNAGNKAQTIARLFGGNADAWAAFDPTTVINRHGPYRDVAGWFAISSQGTPRHDSAMT
                     DAGAMRLVSRDAAANPGNQTAAAYSLCALGRANGIDCAVVSQTGKHDWPFADRSFAAT
                     LPWLAGRLGCPGVTRSALPSTSPPSAFSGPVVTAEHSHDGTR"
     gene            complement(7467..10820)
                     /gene="lysX"
                     /locus_tag="VFI55_03975"
     CDS             complement(7467..10820)
                     /gene="lysX"
                     /locus_tag="VFI55_03975"
                     /EC_number="2.3.2.3"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014390178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     synthetase/lysine--tRNA ligase LysX"
                     /protein_id="HET7073227.1"
                     /translation="MTLASSHPGPHSGPRSSSRPSSRYRWVPAAAGWTVGIIATLSLV
                     ASVSPLIRWVIKVPREFINDYLLNFPDTSIAWSFVLALLAAALTARKRVAWLLLLGNM
                     VLAAALNAADIAAGDNTAAETFGENLGFAVHIVAIVVLVLSYRQFWAKVRKGALFKAA
                     AVLVAGDVIGILASWGLVEMFPGTLAPESRFWYVVNRVVGFSIVDPDAFTGRPHVVLN
                     AIFGLFGALALIAAAIVLFQSQRADNALTGEDESAIRGLLELYGQNDSLGYFATRRDK
                     SVVFAHSGRAAVTYRVEIGVCLASGDPVGDPRAWPQAVDAWLGLCQTYGWAPGVMGAS
                     SQGAQVYREAGLNALELGDEAILRTADFKLSGPDMRGVRQAVTRARRAGLTVRTRRHR
                     DISPEEMTDTIARADAWRDTQTERGFSMALGRLGDPADGDCLLVEALDRDGQVIAMLS
                     LVPWGNTGVSLDLMRRSPQSPNGTIELMVSELALNAEALGITRISLNFAMFRSAFEQG
                     AQLGAGPIARLWRGLLLFFSRWWQLETLYRSNMKYQPDWVPRYACYEDARLFPRVGVA
                     SVIAEGFLVLPFSRREKTHTGHHPAVPARLAESGLLHPDGSAPDMSELQRSAEGDFDD
                     ARSRLPEQVRVRLTKLKTLQRNGVDAYPVGSPPSHTVIRALDADDQETVSVAGRILRI
                     RDYGGVLFAQLRDWSGEMQVLLDNSHLERGHTADFTKAIDLGDLVEITGQMGFSKNGT
                     RSLIVREWRMIGKCLRPLPNKWKGLTDPEARVRTRYVDLAVNPESRELIKARSSVVRS
                     VRETLFAKGFIEVETPILQQVHGGATARPFITHINTYDMDLFLRIAPELYLKRLCVGG
                     VERVFELGRAFRNEGVDFSHNPEFTLLEAYQAHADYLVWIDGCRELIQNAAHAANGEQ
                     VVLRPRADSDGADGCLEPFDISGVWPVKTVHDAVSEALGEHVDPDTPLAKLRKLSDKV
                     HIPYRAHWDAGAVVLELYEHLVEDRTEQPTFYIDFPLSVSPLTRPHRSRRGVAERWDL
                     VAWGVELGTAYSELTDPVEQRRRLQEQSLLAAGGDPEAMQLDEDFLQAMEYAMPPTGG
                     LGVGIDRVVMLVTGRSIRETLPFPLAKPH"
     gene            11269..11865
                     /gene="infC"
                     /locus_tag="VFI55_03980"
     CDS             11269..11865
                     /gene="infC"
                     /locus_tag="VFI55_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET7073228.1"
                     /translation="MSTETRVNERIRVPEVRLIGPGGEQVGIVRIEDALRVAADADLD
                     LVEVAPNARPPVCKIMDYGKYKYEAAQKARESRRNQQQTVVKEQKLRPKIDDHDYETK
                     KGHVVRFLEAGSKVKVTIMFRGREQSRPELGYRLLQRLGADVADYGFVETSAKQDGRN
                     MTMVLAPHRGAKTRARARHPEAPASDSDAAADTDTSPN"
     gene            11905..12099
                     /gene="rpmI"
                     /locus_tag="VFI55_03985"
     CDS             11905..12099
                     /gene="rpmI"
                     /locus_tag="VFI55_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET7073229.1"
                     /translation="MPKAKTHSGASKRFRRTGTGKIVRQKANRRHLLEHKPTKRTRRL
                     DGRTAVSANDTKRVNALLNG"
     gene            12188..12583
                     /gene="rplT"
                     /locus_tag="VFI55_03990"
     CDS             12188..12583
                     /gene="rplT"
                     /locus_tag="VFI55_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET7073230.1"
                     /translation="MARVKRAVNAHKKRRSVLKASKGYRGQRSRLYRKAKEQQLHSLG
                     YAYRDRRARKGEFRKLWISRINAAARANNITYNRLIQGLKAAGVEVDRKNLADIAIAD
                     PAAFTALVEVARAALPEDVNAPSDSGEAA"
     gene            12619..13410
                     /locus_tag="VFI55_03995"
     CDS             12619..13410
                     /locus_tag="VFI55_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET7073231.1"
                     /translation="MLTERSARVASAVKLHRHVGRRRAERFLAEGPNLVEAAFARGLV
                     LEIFVTEAAAQRHASLLASLQAAQTFPVHLVTERAAKALSDTVTPTGLVAVCAMPATR
                     LEDVLAGAPRLIAVAVEIGEPGNAGTLIRIADAMGAGAVILAGHSVDPYNGKCLRAST
                     GSTFSIPVVAVPDAHAAVTALRSAGLQVLATTVDGVTRLDEADLATPTAWLFGAESHG
                     LSAEIAEAADHRVRIAMAGGAESLNVAAAAAICLYQSAQALGCVR"
     gene            complement(13581..>14017)
                     /locus_tag="VFI55_04000"
     CDS             complement(13581..>14017)
                     /locus_tag="VFI55_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073232.1"
                     /translation="VAGQRWIAAGLFEPAVREKAGMHWTAGDEVLLRVFGKLVELAFM
                     AVPDEIRLHPRALAAYRRAQGRVPGDAPLVEAPTFVGPPRDRRGLPMHYFPRGSRPSL
                     RSALDPALQPARALMERAGSLVHTTLSLAGLRPTSRGPGKAA"
BASE COUNT         2468 a         4629 c         4692 g         2228 t
ORIGIN      
        1 aacgcagcgg agaagggatg ggcaaggtgg gcgatcagta tctcgcgacc attcagcccg
       61 attcgtccgg caatcaggtg ctgtacctgc atcaaatcaa ccccgatgga gacgtcgtga
      121 cgttctgcac cgccgcgggg atgaagagtg ccgactcgcc gtgtggcgcc tgacgtgacc
      181 gtgcccgcga cgccgcgtgc tcaccagcgt cgttcgcgcc actcccccag atgggggcgt
      241 tcggcaccca gcgtggtgtc gtccccgtgg ccggggtaga caaccgtctg atcgccgaac
      301 cggtcgaaga cccgcgcctt cacgtcgtcg aacagcgagt cgaactccgc gggtttagtt
      361 gtccgcccca gacctccggg gaacaggcaa tcgccggtga acaactgcgt gacaccgccg
      421 gtcgcgggcc cgtcgagggc cagggcgatc gagccgggcg tgtgcccgcg caggtggatg
      481 acgtcaaagc tcagctcgcc gacctgcacg gtgtcgccgt tggccagcaa acggtccggc
      541 tttaccggca gcggtcccgc gtcgatctcg ttgaccgcgg tgggcgcccc ggtggcggcg
      601 gcgacggcct ccagcgcctg ccaatggtcg aagtgctggt ggctggtgac gatcagcgac
      661 agcttcgggg catagccccg gatcaggccg atgaggacat cggcgtcgtt ggcggcgtcg
      721 atcagcagtg tttctccggt ggccgaacat gtcaccaggt aggcgttgtt gtccatcggg
      781 cccaccgatg ccttcagaat ggtggcgccg ggcagcgtgc ggcgggctgc ggtaccaggg
      841 tcgacgtgtc cggtgtagtg gtcgtgcacg gcagtcatac cgatcacgct actggtgcgc
      901 ggttcgttgt cggtatgggc acatagcatg gaatacggcg tgcgcattta tttctagaac
      961 cgcgtgcggc acgtgggagt ttgagcgaga ggggcagcgt ggctgatcgt ctgatcgtca
     1021 aaggcgcccg tgagcacaac ctgcgcggag tcgacctcga cctgccgcgc gactcgttga
     1081 tcgtcttcac cgggctatcc ggatcgggaa agtcgtcgct ggccttcgac accatcttcg
     1141 ccgaaggtca gcggcgttac gtcgagtcgc tgtccgccta cgcccgccaa ttcctggggc
     1201 agatggacaa gccggacgtc gacttcatcg aggggctgtc cccggcggtc tccatcgacc
     1261 agaagtcgac caaccgcaac ccgcggtcga cggtcggaac catcaccgag gtgtacgact
     1321 acctgcggtt gctgtacgcc cgcgcgggga cgccgcattg cccgatctgt ggcgagcgga
     1381 tcgcccgcca gacgccgcag caaatcgtcg accaggtgct ggcgatggag gagggcaccc
     1441 gatttctggt gctcgctccg gtggtgcgca cccgcaaggg cgaattcgcc gacctgttcg
     1501 acaagctcaa cgcccagggc tacagccggg tacgggtcga cggcgtggtg catccgctga
     1561 ccgatccgcc gaagctgaag aagcaggaga agcacgacat cgaggtggtg gtggatcgcc
     1621 tcaccgtgaa ggcgagcgcc aagcagcggc tcaccgattc ggtggagacc gcgctgaacc
     1681 tggccgacgg catcgtggtg ctggagttcc ccgacgatcg ggacgagcac gaccacgcgc
     1741 gcgagcagcg gttctccgag aagctggcct gccccaacgg gcatccgctg gcggtggacg
     1801 atctggaacc gcggtcgttc tcgttcaact cgccctatgg cgcctgcccg gagtgcaccg
     1861 gcctgggcat ccgcaaggag gtcgaccccg atctggtggt tccggatccg gatcgcacgc
     1921 tggccgaggg cgcggtggcg ccgtggtcga acggccacac cgccgagtac ttcacccgga
     1981 tgatggccgg gctcggcgag gagctcggat tcgacgtgga cacgccctgg gccaaactgc
     2041 cggccaaggc ccgcaaggca atcctggagg gttccgacca ccaggtccat gtgcggtacc
     2101 gcaaccggta cggccgcacc cggtcgtact acgcggattt cgaaggggta ctggcgttcc
     2161 tggagcgcaa gatggcccag accgagtccg agcagatgaa ggaacgctac gaaggtttca
     2221 tgcgtgacgt gccctgcccg gtttgcgagg gcacccggct caagccggag attctggcgg
     2281 tgacgctggc cggcgaatct gggggggagc gggacgcgaa gtccatcgcc gaggtctgcg
     2341 agctctccat ttcggactgc gcggattttt tgaacgcgct caccctgggt cagcgcgagc
     2401 aggcgatcgc cggacaggtg ctcaaggaga ttcagtcgcg gctcggcttt ctgctcgacg
     2461 tgggcctcga gtacctgtcg ttgtcgcgcg cggcggccac gctgtccggc ggtgaggcac
     2521 aacgcatccg gttggccacc cagatcggct ccggcctggt cggtgtgctc tatgtgctgg
     2581 atgagccgtc catcgggctg caccagcgcg acaaccgtcg tctcatcgaa accctcaccc
     2641 gcctccggga tttggggaac acgctgatcg tcgtggagca cgacgaggac accatcgcgc
     2701 acgccgactg ggtcgttgac atcggcccgg gcgccggcga acacggcggc cgcatcgtgc
     2761 acagcggaac ctacaaagac ctgctggtca gcaaggactc gatcaccggc gcctacctgt
     2821 ccggcaagga aagcatcgag atccccgcga tccggcgccc cgtcgaccgg cgacgtcaac
     2881 tcaccgtggt cggcgcccgc gagcacaacc tgcgcgatat cgacgtgtcg tttccgctcg
     2941 gtgtgctgac ctcggtgacg ggcgtgtccg gctcgggcaa gtcgacactg gtcaacgaca
     3001 tcctggcggc ggtgctggcc aaccggctga acggcgcccg gcaggtcccg ggccggcata
     3061 cccgggtcac cgggctggac cacttggaca agctggtgcg ggtcgaccag tcgccgatcg
     3121 gccgcacgcc gcgatccaac ccggccacct acaccggtgt cttcgacaag atccgcaccc
     3181 tgttcgcggc caccaccgag gccaaagtcc gtgggtatca acccggccgg ttctcgttca
     3241 acgtcaaggg cggtcgctgc gaggcatgca cgggcgacgg caccatcaag atcgagatga
     3301 acttcctgcc cgacgtgtat gtgccctgtg aggtctgcca cggggcccgc tacaaccgcg
     3361 aaaccctcga ggtgcactac aagggcaaga ccatctccga ggtgctggac atgtcgatcg
     3421 aggaagccgc ggaatttttc gagccgatca gcggcattca ccgctacctg cgcacgctgg
     3481 tcgacgtcgg cctgggctac gtccggctcg gccagcccgc gccgacgctg tccggcggtg
     3541 aggcgcagcg ggtcaagctg gccgccgagc tgcagaagcg ttccaccggg cgcaccgtct
     3601 acatcctcga cgaacccacc accggattgc atttcgatga catccgcaag ctcttgaagg
     3661 tcatcaacgg cctcgtcgac aaaggcaaca cggtcatcgt catcgaacac aacctggacg
     3721 tgatcaagac gtcggactgg atcgtcgaca tggggccgga gggcggcgcc gagggcggaa
     3781 ccgttgtcgc ccagggcact ccggaggaca tcgccgccgt gcccgaaagc tacaccggga
     3841 agtttctcgc cgaggtggtc ggtgacggag cggcgagccc gcaacggtcg acccgccgcc
     3901 gaaaagccag cgcctgagtc tgctgtcacg gcgacgaccc cgtgcccagg gcgggatttc
     3961 accgcgccgg tttgctcgcg ctgagcacgg gctcgatcag cccgtcgacc cagctttgga
     4021 cagcggcgac gggaagcgcg ggctgatccg cacgccgtcc agccacgaag tcagcccgtc
     4081 ggcgcgtcgc tgcagggcac gccgcccgtc accaccgggg tcctcggtca actgcaactg
     4141 gacccgggcg ctgtcgtctt ggtaccagcc gcccaccacg cgtccgttcc accacgcggt
     4201 gggtccggcg ttgccgttgc ggtcgaaaac ctgggcgcga tgttcgccga ggtaccaatc
     4261 gcgctcaaac cagcccatcg tggtgacgtc cagccccggc agcagcgcgt cccaaggtgc
     4321 cacagcgggt tccggctcga ggtcgtcggg gagcgcgtag ccgtcgctgc catgcagatc
     4381 gactttcacc gcgccgatgt cggccaacgt ttttcgggcg gccgtcagcg tgatgccgaa
     4441 ccaccacttg atgtcggcgg cggtggcggg tccaaatgtc cgtagccacc ggcggatcag
     4501 ctcggcctgc gcggcctggg cgggagggac ctcgaaggga cggccgagcc agtccgcggt
     4561 ggtcgcccag cgcggacgcg atgtggtcca gctaccgtcg ttgggcccgc gcacgatgtc
     4621 gcctcgcgcc gagagcagtg tcagcacccg cggggcaagc ggaaccgatc caccccaacg
     4681 ctttccaggc gcggggtcgt aggttcctgc cagttcgggc agcgcggccc gcagttcggt
     4741 gctgctggcg ggctcgttgt caccgaggtg gcgcaacacc gcggtgcagg ccagttcgag
     4801 ccagcggtcg ccgtcgtagg ccaccccggc cttgtggacg tcagcggcca gtcggcgacg
     4861 ttcgttggtg gcgacgcggt tgctgaatgc cgactggatc atcgccaagt cgtcgccgtt
     4921 caccaaccac agtgtccgtc gcatcgccaa atgccgcacc acagcacgct tttggtatag
     4981 ctcctgctcc aggtcggcga cggcgaactc cggggtccgc gcccacagcg acaggtaggg
     5041 cgtggcgggg tcggtagcgt gcagtccgac cagggcggcg gcgacttccg ctatcggtcg
     5101 tgagccggtg cgcgccagga aatgccggcg cgccaggcgg ttgcgccgct cagtgatggt
     5161 gaatctgcgc acggttgaat cgtcggcggc gtcgtggacc ctcagcgctc ctcgggtcgc
     5221 atcgattcgc ggatctgcgc caaccgctcg gccgccgcgc gtcggcgctc ttcgaactgc
     5281 tcctcgacgg agcggccttc ggccgtctcg gcgtcgagtt cggccgcacc ctgcgccgtc
     5341 gcgtagcggg tttcgatctt ctcgcggacg gattcaaagg tcggcacacc ggcgctgtcg
     5401 tacccggcgc cgggctcgga ggtgtccgga gttgctgatt cgtcgggcat tccgccacgc
     5461 tactgccgcg gtgtgtcttt cgcccgtggc tgccgatcga aatagcggac catctgttcc
     5521 cagtacacca gcgtcagggc catctgcacc gcggacgcga tcagcgccgg aatcatccgg
     5581 tgacggcggg cggcgaggac accgcacagc gcggccgcgg acgtcgccac gctgacgagc
     5641 atcgctccat caccgcgtcg acggctgatc cacagttcct cgcccagcat tgcccgagtc
     5701 gaccaggcac gttcatcggc gggcttgccg aagacgaacg gattgatggc cagccacagc
     5761 gcgatccaca gcccatggct ccagcgccgc gtccacacgg gcaccagtat cagcggtgtg
     5821 ctcgcccagc gtgtccacgc actccacgga ttgcagtgcc gcgcgaagat cgcccgccga
     5881 atgcgcgcaa cgtgcggctt cagggcggtt accgggtgcc gtcgtgggag tgctcggccg
     5941 tcaccacggg cccggaaaac gccgacggcg gggacgtgct gggcaacgcg ctgcgcgtca
     6001 cccccggaca gcccagccgg ccggccagcc agggcaacgt ggccgcgaag gaccggtcgg
     6061 cgaatggcca gtcgtgctta cccgtctggg agaccaccgc gcagtcgata ccgttggcac
     6121 gaccgagcgc gcacagtgaa taggcggccg cggtctggtt gcccgggttg gcggcggcgt
     6181 cacgactgac cagccgcatt gcgccggcgt cggtcatcgc ggagtcgtgt cgcggcgtgc
     6241 cctgtgacga gatcgcgaac cagccggcca cgtcgcggta agggccgtgc cgattgatca
     6301 ccgtggtcgg atcgaatgcc gcccaggcgt cggcgtttcc gccgaacagc ctcgcgatgg
     6361 tttgcgcctt gtttccggca ttcgggaaaa agtcaccggc gatgtcgacg aacgcgctga
     6421 acatgtcggg gtgcatgacg gtcaggtcca cggcgcaggt tccgcccatc gaccagccga
     6481 cgacgcccca gttggaccgg tcccggctga cgccgaaatt cgaaatcatg taggggacga
     6541 cgtctttggt gagatggtcg gccgcgttgc cgcgaacccc gttgacgcat tcggtgtcgt
     6601 tgtcgaacgc gccgccaggg tcgacgaaca ccaataccgg cgcgttgccg tcgtgggtgg
     6661 ctgcgaagtc gtcgatcgtc ttgaccgcat tgcccgcgcg cgtccagtcc gcgggcgtgt
     6721 tgaattgccc accgatcatc atcaccgtcg gcagcgccgg cggcggactg ctggtgaacc
     6781 atgcgggcgg caggtacacc agttcaccgc ggtgcttgaa atgcgatgcg tccgacggga
     6841 tcaccaccgg taccacgctg ccgtgcggtg gccgggcccg cttgccgacc attgccgtga
     6901 cggtggcttg gtcggtttga tcgggcaccg gaccgtacgt gagcgcgttc cacgcggacg
     6961 gcacggtggg gaagtagccg acccacagat tgagcaccag tgccgcgcaa agcaaacaca
     7021 tcggaacggc aagcagcgtc gcgccacgcc gccacggttc cgcgtggcgc cagcccagga
     7081 ccaacacggc cgcggccatg ccggtcaacg cgacccagac ccacagcgtg gtcggcgccg
     7141 gttcgtcgga caggctgcga tcgacgatgt accagtgcgt tgcatagacc gcgccgccgc
     7201 ccaccagacc ggccgccggc aaccagatca tccgccagcg ccgcgagcgc cagccgatac
     7261 ccagcgtcag cacgatagcc gtggtgatct ggatggtcgc gggtatccaa ccatgcatca
     7321 gggaggtgaa attgctcgcc agcaactgcg ccgtggcagg gggtatcgac gccgtcacgt
     7381 ggtccattgt gagctattgt taaccagcct ggaacatatt cgcgcaaggg ctcgccgcga
     7441 attacctcta gcggccgcgc gctcgcttaa tggggtttag ccagcggaaa tggcaaagtc
     7501 tcgcgaatgc tgcggccggt tacgagcatg acgacccgat cgatgcccac gccgagcccg
     7561 ccggtcggcg gcatcgcgta ctccatggcc tgcaggaagt cttcgtcgag ctgcatcgct
     7621 tcggggtctc cgccggcggc cagcagcgac tgttcctgca gccggcgccg ctgctcgacc
     7681 ggatcggtga gctcgctgta ggcggttccc agctcgacac cccaggccac cagatcccac
     7741 cgctcggcga caccgcgccg actgcggtgc ggccgggtca acggagacac ggacagcgga
     7801 aagtcgatat agaaggtcgg ttgctcggtc cggtcctcga cgaggtgctc gtacaactcg
     7861 agcaccaccg cgccggcgtc ccaatgcgcc cgataaggaa tgtgcacctt gtcggacagc
     7921 ttgcgcagct tggccagagg ggtgtctggg tcgacgtgtt ctccgagcgc ttcggacacc
     7981 gcgtcgtgca cggtcttgac cggccagaca ccggagatgt cgaacggttc gaggcagccg
     8041 tcggcaccat cggagtcggc gcggggtcgc agcacgacct gctcgccgtt ggcggcgtga
     8101 gcggcgttct gaatgagctc gcgacagccg tcgatccaca ccaggtagtc ggcatgggcc
     8161 tggtaggcct ccagcaaggt gaactccgga ttgtggctga agtcgacgcc ctcattacgg
     8221 aaggcgcgcc ccagctcaaa gacccgctcc acgccaccga cgcacaaccg cttcagatag
     8281 agctcggggg cgatgcgtaa gaacagatcc atgtcatagg tattgatatg ggtgatgaac
     8341 ggccgcgccg tcgccccgcc gtggacctgt tgcaggatcg gggtctcgac ttcgatgaac
     8401 cccttggcga acagcgtttc cctgacggag cgcaccacgc tgctgcgggc cttgatcagc
     8461 tcacgcgact ccggattcac cgccaggtcg acgtagcggg tccgcactcg cgcctcgggg
     8521 tcggtcagcc ccttccactt attcggcaac ggccgcaaac atttaccgat cattcgccac
     8581 tcgcgcacga tcagcgagcg ggttccgttc ttgctgaagc ccatctgccc ggtgatttcg
     8641 accaaatcac caagatcaat ggccttggtg aaatcggcgg tgtggccacg ctccagatgc
     8701 gaattgtcca gtagcacttg catttcgccc gaccagtccc gcaactgggc aaacaacacg
     8761 ccgccatagt cgcggatccg caatatccgg ccggccaccg agacggtctc ctggtcgtcc
     8821 gcgtccagcg cccgaatgac cgtatggctt ggcggcgatc ccaccgggta ggcgtcaaca
     8881 ccgttgcgct gcaaggtttt cagcttggtg aggcgcaccc gcacctgttc tggaagacga
     8941 gatcgtgcat cgtcgaagtc gccctctgcg ctgcgttgca gctcactcat gtccggtgcc
     9001 gacccgtcag ggtgcagtag cccggactcc gccaatcggg ccggcacggc ggggtgatgc
     9061 ccggtgtgcg tcttctcccg ccggctgaac ggcaggacaa ggaaaccttc cgcgatcacc
     9121 gaggcgacac cgacgcgggg aaacagcctg gcgtcctcat agcaggcgta gcggggaacc
     9181 caatcgggtt ggtacttcat gttggagcgg tacagcgtct ccagctgcca ccaccgcgag
     9241 aagaacagca acaggccccg ccacaaccgc gcaatcggac cggcgcccag ttgggcaccc
     9301 tgctcgaagg cagaccggaa catcgcaaag ttcaatgaga tacgggtgat cccaagggct
     9361 tccgcgttca gggcgagctc gctcaccatg agttcgatgg tgccgttggg ggattggggt
     9421 gaacgccgca tcaagtccag ggagacgccg gtgttccccc acgggaccag cgacagcatc
     9481 gcgatcactt ggccgtcgcg gtccaacgcc tcgaccagca ggcagtcccc gtcggccgga
     9541 tcaccgagcc ggccgagtgc cattgaaaag ccgcgttcgg tttgggtgtc acgccaggcg
     9601 tcggcgcggg caatggtgtc ggtcatctct tcgggggaga tgtcgcgatg tcgccgggtg
     9661 cgcaccgtca gtcccgctct gcgggctcgc gtcaccgctt gacgcacccc gcgcatgtcc
     9721 gggccggaca gcttgaaatc agcggtgcgc aaaatcgcct cgtcgcccag ctcgagcgcg
     9781 ttgaggccgg cctcgcggta cacctgggct ccctgtgagc tggcgcccat gacgccgggc
     9841 gcccagccat aggtctggca caaacccaac cacgcgtcca cggcctgggg ccaggcgcgc
     9901 gggtcgccaa cggggtcgcc gctggccagg cagacgccga tttcgactcg gtaggtgacg
     9961 gcggcgcggc cactgtgtgc gaacaccacc gacttgtcgc ggcgcgtggc gaagtagccc
    10021 agcgagtcgt tttgcccgta cagctcgagc agcccgcgga tggcggactc gtcctcgccg
    10081 gtcagcgcgt tatcggcgcg ttgggactga aacagcacga ttgccgccgc gatcagcgcg
    10141 agcgcgccga acagtccgaa gatcgcgttg agtacgacgt gcgggcgccc ggtgaacgcg
    10201 tccgggtcga cgatggagaa tccgaccacc cggttgacca cataccaaaa gcgggactcg
    10261 ggcgcgaggg tgcccgggaa catctcgacc aggccccagg atgccaagat tccgatcaca
    10321 tccccggcga ccagcaccgc cgccgccttg aagagcgcgc ctttgcggac cttggcccag
    10381 aactgccgat acgacagcac cagcacgacg atcgcgacaa tgtgcacggc gaagccgaga
    10441 ttctcgccga acgtctccgc ggcggtgttg tcgcccgcgg cgatgtcggc ggcgttcaat
    10501 gcggcggcaa ggaccatgtt gccgagcaac agcaaccacg cgacgcgttt gcgcgctgtc
    10561 agcgcggcgg ccagcaatgc cagcacgaac gaccacgcga tgctggtgtc agggaagttg
    10621 agaaggtagt cgttgatgaa ttcccgtggg accttgatca cccaccggat cagcggcgac
    10681 acgctggcga ccagcgacag tgtggcgatg atgccaaccg tccacccagc cgccgccggc
    10741 acccaccggt accgagagct cggccggctg ctcgaacgag gccccgaatg gggtcccgga
    10801 tgagaagaag cgagtgtcac agaccgcgag gatatgcctt taacccgtga aaatcctggc
    10861 gaagcgccat gagtcgcgac gacggattag gcgggtttgc tgaacggggg taatagccgt
    10921 caaacgctcg tcccgccgga gaagtgctgg atgacggctc atgactgcta aactagcgac
    10981 tgcgaccatc ccggcgaatg ccggggacag ttaagtggag tcccactccc accggtagcc
    11041 acgacaaagc ttctcggctt ttccaggcta ttcggtccgg tcacaggcat cgtgcatgcc
    11101 tcgttccgcg ggtagcgcgg gcggcttgag tggtttcagg ccgtgatcgg tcggcattgg
    11161 gccctgctta tgcagggctt tttttgctga tggtgtggtg tttccaccgc tgaatccgga
    11221 cgggcttggg actggccgcg ccgacagaag cccaacaagg gaggccccat cagcactgag
    11281 acccgcgtca acgagcgcat ccgcgtacct gaagttcgat tgattggccc gggaggggag
    11341 caggtaggca tagtgcgcat cgaagacgca ctgcgcgtcg ccgcggacgc cgatctcgac
    11401 ctcgttgaag ttgccccgaa cgccagaccc ccggtctgca aaatcatgga ctacggcaag
    11461 tacaagtacg aggcggcgca gaaggcgcgc gaatcccgcc gcaaccagca gcagaccgtc
    11521 gtcaaagagc aaaagctccg accaaagatc gacgatcacg actacgagac caagaagggt
    11581 cacgtggtcc gctttctgga agcgggatcg aaggtaaagg tgaccatcat gtttcgcgga
    11641 cgggagcaat cgcggcccga gctgggctac cggttgctgc aacggctggg tgcggacgtc
    11701 gccgattacg gattcgtcga gacgtcggcc aagcaggacg gccggaacat gacgatggtg
    11761 ctggcaccgc accgcggcgc gaagactcgt gccagggcgc ggcatccgga agcaccggct
    11821 tcggattcgg acgcggcggc cgacacggac acttcaccca actgacctga cagcgataac
    11881 gacgaaacaa gaactagagg aaacatgccc aaggccaaga cccacagcgg ggcttcgaag
    11941 cgattccggc gcaccgggac cggaaagatc gtccgccaaa aagccaatcg ccggcatctg
    12001 ctcgagcaca agccgaccaa acgaacccgg cggctggacg gccgcacggc ggtatcagcc
    12061 aacgacacca agcgggtcaa cgcgctgctg aacggctgac cctcgatcta ccgagcgccg
    12121 caagaccgtt gaacacgggg cggcgcatcc tttgaccact tacgtttgaa cgagaatagg
    12181 aacatccatg gcacgcgtga agcgggcggt caacgcccac aagaagaggc gcagcgtcct
    12241 gaaggcctcg aaaggctatc gcggccagcg atcccgcctc tatcgcaaag ccaaagagca
    12301 gcagctgcat tcgctgggct atgcctatcg cgaccgccgt gcgcgcaagg gcgaattccg
    12361 caaattgtgg atctcgcgga tcaacgctgc tgcgcgcgcc aacaacatca cctacaaccg
    12421 gctgatccag ggcctgaagg ccgccggcgt cgaggtggac cgcaagaacc tcgccgatat
    12481 cgcgatcgcc gatccggcgg cgttcacggc gctcgttgag gtcgcccggg cggcactgcc
    12541 cgaagacgtc aacgctccat cggattcggg agaggctgct tagcccggca atcagcaaac
    12601 ctcgagacga tgccggcgtt gctcaccgaa cgctcggccc gggtggcctc ggcggtcaaa
    12661 ctgcatcggc acgtcggccg ccggcgtgcg gaacggtttc tcgccgaggg cccgaacctg
    12721 gtcgaggccg ccttcgcacg cgggttggtg ctggagatct ttgtcaccga agctgctgcg
    12781 cagcggcacg cttccttgct ggcgtctttg caagcggcac agacgtttcc ggttcatctg
    12841 gtaaccgagc gggccgcaaa agcgctctcg gacaccgtca ctccaacggg attggtagcg
    12901 gtgtgcgcga tgccggcgac gcggctggag gacgtgttgg ccggcgcgcc ccggctgatc
    12961 gcggttgccg tcgagatcgg cgagccgggc aatgcgggca cgctgattcg catcgccgac
    13021 gcgatgggcg cgggggcggt gatcctcgcc gggcacagcg tcgatccgta caacggcaag
    13081 tgtctacgcg catcaacggg cagcactttc tcgattccgg tcgtcgccgt gcccgacgcg
    13141 cacgccgcgg tcaccgcgtt gcggtccgcc gggctgcagg tgctggccac cacggtggac
    13201 ggggtgacgc gcctcgacga ggccgacctc gccacgccga cggcctggtt gttcggggcc
    13261 gaatcacacg gtctgtcggc cgagattgcg gaagcggccg accaccgcgt gcggatcgcg
    13321 atggccgggg gcgccgagag cctgaacgtg gcggccgccg cggccatctg cctgtaccag
    13381 agcgcgcagg ccctggggtg cgttcgctga gcggcgttac ggtccgcgtg cctcactcat
    13441 cgagtctgtg ctgagggatt cgactctgcg tgtagggccg actgaagccg gggatcgcgc
    13501 gcccatcgca gactcgatgg cgccgagccg tcagacgctc aggtgacgga agtgacagcc
    13561 tgcgtccgat ccatccgccc tcacgcagct tttccgggcc cgcgagaggt gggccgcaag
    13621 ccggccagcg acagcgtggt gtgcaccagc gatccggcgc gttccatcag cgctcgagca
    13681 ggttggaggg cggggtcaag ggcggagcgc agcgaggggc gcgaaccccg gggaaaatag
    13741 tgcatcggca gcccgcggcg gtcacgcggc ggccccacga acgtcggtgc ctcgaccaac
    13801 ggcgcgtcgc cgggaacgcg tccttgcgca cggcggtatg ccgccagcgc gcgggggtgg
    13861 agccggattt cgtcgggaac cgccatgaag gccagctcga cgagtttgcc gaacacgcgc
    13921 agcagcacct cgtcgcccgc cgtccagtgc attcccgcct tctcgcgaac cgcgggctcg
    13981 aagagtcccg ccgcgatcca gcgctggccg gccacca
//
LOCUS       DASSGQ010000087         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1343106,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000087 DASSGQ010000000
VERSION     DASSGQ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343106"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFI55_04005"
     CDS             <1..249
                     /locus_tag="VFI55_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7073233.1"
                     /translation="RLVGDVLTAITATYRDDVDTAWQRAVTAGAAVVYPLADQFYGER
                     AGWLRDPFGQQWMLSRRIETVPAEEMTRRAAAFFHQSG"
     gene            complement(407..1780)
                     /locus_tag="VFI55_04010"
     CDS             complement(407..1780)
                     /locus_tag="VFI55_04010"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET7073234.1"
                     /translation="MGDDVMISVDPHKASNTLAVMDPVTKTVIARQRFANTAVGYRRL
                     REFAGGWTHRRWAVEGCHGAGRSLAQRLIGDGEIVLDVPAKLAARVRVYSQGHGRKTD
                     ADDAISIGLAALNSTRVAVVRRDDTLVSLRLLCDRRDELGALRTQAVCRLHRLLCELT
                     PGGTHRGLRASRAEQLLVKLRPVDPVDVVRCQLAHQHLDDIRALDAKMTAVRAQIVAL
                     VDATGTRLTELYGIGPIIAGRILAEVERIDRFPSRHHFASYNGTAPIDASSGEQVRHR
                     LSRAGNRRLNHALHMMAVTQIRQPETMGRGYYERKRLEGKTPKEAMRCLKRRLSDVVY
                     WQLVADRRATITQCGSATTPTRTRPTSTSATTPVPRIAPLRRPPHPTVSTGSSHSTGK
                     TAASSASKSSTPAVDSPKTSSTKQKTRAEATRKPRTNSAAVPDRRPTSAPAAGVKVGA
                     ATRRARP"
BASE COUNT          358 a          712 c          686 g          363 t
ORIGIN      
        1 cgtctggttg gcgacgtgct caccgcgatc acggcgacct atcgggacga cgtcgacacg
       61 gcctggcagc gggcagtcac ggccggcgcc gcggtggtgt acccgcttgc ggatcagttc
      121 tacggcgagc gggccgggtg gctgcgcgat ccgttcggac aacagtggat gctcagccgg
      181 cgcatcgaga ccgtgccggc cgaggagatg acgcgccggg ccgcggcatt cttccaccaa
      241 tccgggtgac gcacgtcgat ccgctcatag ttgaatgccg gcacgcggcc ccggtaaacg
      301 atgatcagcg actgctcacg ccaagacgat cttgtatcgg cacctctctg tcaagagctg
      361 tggcagctgt tcccgcccgg ggattgtgtc gtcgtcctcg ccggggtcaa ggccgagccc
      421 ttcgggtggc tgcgccaacc ttgaccccgg ctgcgggcgc cgaggttggg cggcggtcgg
      481 gaacagcggc ggagttggtc cttggcttgc gcgtcgcctc agctcgcgtc ttctgcttcg
      541 tcgaggatgt cttgggtgag tcgactgctg gcgtcgagga tttcgatgcc gacgatgcgg
      601 ccgtcttccc agtcgagtgc gacgaacccg tcgacaccgt cgggtgtggc ggcctgcgta
      661 gtggtgcgat gcgcggcacc ggggtcgtcg ccgaggtgga tgtaggccgc gtccgcgtcg
      721 gggttgtagc tgatccgcac tgggtgattg tcgcgcgccg gtcggcgacc agctgccagt
      781 acacgacatc ggacagccgc cgcttcaggc agcgcatcgc ctctttcggt gtcttgccct
      841 cgagccgttt gcgttcgtaa tagccgcggc ccatggtttc tggttggcgg atctgtgtta
      901 ccgccatcat gtgcagcgcg tggttgagcc gccggttccc ggcgcgggag agccgatgcc
      961 ggacctgctc accagaggag gcgtcgatgg gtgcggtgcc gttgtaagac gcgaaatgat
     1021 gccgcgacgg gaaccgatcg atccgctcca cttcggccag gatccgcccg gcaatgatcg
     1081 gcccgatgcc atacagctcg gtgagccggg tcccggtggc atcgaccagg gcgacaatct
     1141 gcgcccggac cgcggtcatc ttcgcgtcca acgcccgaat atcgtccagg tgctgatggg
     1201 ccagctggca gcgcaccaca tcaaccggat caacgggacg cagcttgacc agcaactgct
     1261 cggcacgtga cgcccgaagc ccacgatggg tcccgcccgg agtgagctcg cacaacagcc
     1321 gatgcaaccg gcacaccgcc tgggtgcgca acgcgcccag ctcatcgcgg cgatcgcaca
     1381 gcagccgcaa gctgaccagc gtgtcatcac ggcgtaccac cgcaacgcgc gtgctgttca
     1441 gcgcagccag accgatcgag atcgcgtcat cggcgtcggt cttgcggccg tgcccttgcg
     1501 agtacacccg aacgcgagca gccaacttgg ccggcacgtc gagcacgatc tcaccatcac
     1561 caatcagccg ctgcgccagc gaccggcccg cgccatggca accctctacc gcccagcgcc
     1621 gatgcgtcca gcccccggcg aactcccgta gccggcgata cccgacagca gtgttcgcaa
     1681 accgttgccg ggcaatcacc gtcttggtga ctgggtccat cactgccagc gtgttacttg
     1741 ccttatgcgg atcgacgctg atcatcacat cgtcgcccat cacaccgcct cctagctcga
     1801 acgggacaac tgcgtggcga ggagggcaat gcaactaccg gcgacgcaga cctctcaaga
     1861 gccacaaccc cgcgggcgga cccggtaagc cgcaggccgt tcgtgagcca gaacgcacca
     1921 gacgacgacg gcagccgatt cgcgagcgaa ctcaccgagc ccaacgcctg agactgctac
     1981 tcccaggctc ccgacgtcga gtcaaacaag ttgccgattc ggtacgtatc ttctggatgc
     2041 aagacggcgc ggcgtgtcgt tgttctgtgc gcaatgttgg gattgacgat ccggtctcgc
     2101 gcatcggcga ctgcacacc
//
LOCUS       DASSGQ010000088         3045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_796226,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000088 DASSGQ010000000
VERSION     DASSGQ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796226"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VFI55_04015"
     CDS             complement(<1..207)
                     /locus_tag="VFI55_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7073235.1"
                     /translation="MIDLLVVGGGPAGLATALNGARAGLEVVVVERRAGLIDKACGEG
                     LMPHTLRHIARLGVHPHGRPFRGIR"
     gene            complement(204..713)
                     /locus_tag="VFI55_04020"
     CDS             complement(204..713)
                     /locus_tag="VFI55_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET7073236.1"
                     /translation="MTWYGLLIGAVAVERLIELVLANRHRAWSRARGGVEFGARHYPA
                     LVALHAALLAGCLAEAIGLHRPFIPAVGWPMLTLVAAAQALRWWCISTLHEQWNTRVI
                     VIPGAARIDGGPYRLVPHPNYIAVIIEGFALPLVHGAWITALVFTVLNAWLLKARIGV
                     ENKALASLT"
     gene            complement(716..1822)
                     /locus_tag="VFI55_04025"
     CDS             complement(716..1822)
                     /locus_tag="VFI55_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HET7073237.1"
                     /translation="MRGPLSGNRFRAHKASSVIAGVHGVLPEHRYAQSEITESLATLP
                     AFENYADALRKFHASSKVNSRYLVLPLERYASLTDFGDANDIFVENAVELGCAAVSGA
                     LDDAGLRPADVDVIYSTTVTGLAVPSLEARIAARLGLRPDVRRVPMFGLGCVAGAAGV
                     GRLNDYLRGAPDDVAVLLSVELCSLTFPATGPSPASLVGTALFGDGAAAVVAVGERRA
                     RKIGAAGPDVLGSRSHLYPDSQNTMGWNIGASGFGLVLSPDVASVIERHLADDVTQFL
                     AARGLSVPDVGAWVSHPGGPKVIEAIVASLGLPEDALELTWRSLSEIGNVSSSSVLHV
                     LRDTIAKQPPSGSPGVMLAMGPGFCSELVLLRWR"
     gene            complement(1819..>3045)
                     /locus_tag="VFI55_04030"
     CDS             complement(1819..>3045)
                     /locus_tag="VFI55_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET7073238.1"
                     /translation="PRPARKPVEDEFWYRSTNFVTRRAVPVGVAVVAVLLLLGAPFLG
                     VRWGFPDDRVLPDSASARQVGDQLRSDFANDSATDVSVVIPDSDGLSDAEFTRYAASL
                     SLVQGVSAVSAPVGTFTHGALAGPPAAPTGASQGSAFLTVRSTAPLFSDASERQLRRL
                     HDVPGPGGRKVEFAGTAQTNRDSVNAITSRLPLVLGLIALVTFVLLFLLTGSVVLPLK
                     ALVLNVLSLTAAFGAMVWIFQNHHLGGLGTTTTGTLVANMPVLLFCIAFGLSMDYEVF
                     VASRIREFWRASGQTRADNDESVALGVARTGRVVTAAALVMSISFAALIAAHVSFMRM
                     FGVGLTLAVVVDATLVRMVLLPTFMHVLGTWCWWAPRWLTRIHQRFGISEERHPAATE
                     PVPHAVPEKVARKVSK"
BASE COUNT          543 a         1070 c         1019 g          413 t
ORIGIN      
        1 cctgatgccc cgaaacggtc tgccgtgcgg gtgcacgccg aggcgtgcga tgtggcgcag
       61 ggtatgcggc atcaggccct cgccgcaggc cttgtcgatc agcccggcgc ggcgttcgac
      121 gaccacgact tcgagcccgg cgcgggcgcc gttgagggcg gtggccaggc cggcgggccc
      181 accgcccacg accaggaggt cgatcatgtc aggctcgcca acgctttgtt ctccacaccg
      241 atccgcgctt tcagtagcca cgcgttgagc accgtgaaca ccaacgcggt gatccacgcg
      301 ccgtgcacca gcggcagcgc gaacccctcg atgatcaccg cgatgtagtt cgggtgcggc
      361 acaaggcgat acgggccacc gtcgatgcgt gccgcaccgg ggatgacgat cacccgggtg
      421 ttccactgct cgtgcaacgt ggagatgcac caccagcgca gcgcctgcgc ggcggccacg
      481 agcgtgagca tcggccagcc caccgccggt atgaacgggc ggtgcagccc gatcgcctcg
      541 gccaggcatc ccgccagcag ggcagcgtgc agcgcgacca gcgcggggta gtggcgggcg
      601 ccgaattcga cgccgccgcg cgcccggctc caggcgcggt gacggttggc cagcacgagc
      661 tcgatcagtc gctccaccgc aaccgcgccg atcagcagcc cataccaggt catgatcaac
      721 gccaccgcag cagcacgagt tcggagcaga accccggacc catcgccagc atgacgcccg
      781 ggctgccgct cggcggctgc ttggcgatcg tgtcacgcag cacgtgcagc accgacgacg
      841 acgagacgtt gccgatctcg ctgagcgacc gccacgtcaa ttcgagtgcg tcctcaggca
      901 atccgagcga cgcgacgatt gcctcgatca ccttcgggcc gcccgggtga ctgacccacg
      961 cccccacgtc ggggacgctc aacccgcgcg ccgccaggaa ctgcgtgacg tcgtcggcca
     1021 ggtgtcgttc gataacgctc gccacgtccg gagagaggac gaggccgaat cccgacgctc
     1081 cgatgttcca gcccatcgtg ttctgcgagt ccgggtacag gtggctgcgc gaaccgagga
     1141 cgtcgggccc ggccgccccg atcttcctcg cgcgacgctc gccgacggcg accaccgccg
     1201 cggcgccgtc gccgaaaagc gccgtgccga caagcgaggc cggcgaggga cccgtcgcgg
     1261 gaaaggtgag cgagcacagc tccaccgaga gcaggaccgc gacgtcgtcg ggagcgccgc
     1321 gcaggtaatc gttgagtcgg ccgaccccgg cggcgccggc gacgcaaccc aaaccgaaca
     1381 tcggcacccg ccgcacgtct gggcgcagcc cgaggcgcgc cgcgatgcgg gcctccagcg
     1441 aggggaccgc gagaccggtg acggtcgtgg agtaaatgac gtcgacgtcc gccggacgca
     1501 atcctgcatc atcgagagcg cccgacaccg ccgcgcaccc gagctccacc gcgttttcca
     1561 cgaagatgtc gttggcgtcg ccgaagtcgg tcagcgaggc gtaccgctcg agaggcaaca
     1621 ccaggtagcg gctgttcact ttggaactcg cgtggaattt ccgcagcgcg tcggcgtaat
     1681 tctcgaacgc cgggagcgtg gcgagcgact cggtgatctc gctttgcgcg taacggtgct
     1741 ccggcagcac gccgtgcacg cccgctatga cgctgctggc cttgtgggca cgaaacctgt
     1801 tgccgctcag aggtcccctc atttgcttac ctttctcgca accttctctg gaactgcgtg
     1861 aggtaccggc tccgttgccg ccggatggcg ctcctcgctg atgccgaagc gctgatggat
     1921 ccgggtcagc caccgaggtg cccaccagca ccaggtgccc agcacgtgca tgaaagtcgg
     1981 cagcagcacc atgcggacca gcgtcgcgtc cacgaccaca gccaatgtca ggccgactcc
     2041 gaacatccgc atgaatgaca cgtgcgcagc gatcagcgcc gcgaacgaga tcgacatcac
     2101 cagcgccgcg gccgtcacca cccggcccgt ccgggccacg ccgagcgcga cgctttcgtc
     2161 gttgtcggcc cgggtttgtc ccgacgcgcg ccagaactcg cggatccggg aagccacgaa
     2221 gacctcgtag tccatcgaca ggccgaaggc tatgcagaac agcagcaccg gcatgttggc
     2281 gaccaaggtt ccggtagtcg tggtcccgag gccaccgagg tggtggttct ggaatatcca
     2341 gaccatcgcg ccgaatgccg cggtcagcga aagcacattg agcacaagag ctttgagcgg
     2401 caatacgacg ctgccggtca gcaggaacaa caacacgaac gtcaccaggg caatcaggcc
     2461 cagcaccagc ggcaggcgcg acgtgatcgc gttgacgctg tcccgattgg tctgtgcggt
     2521 cccggcgaat tcgaccttcc ggccgccggg accggggacg tcgtgcagac ggcgcagctg
     2581 gcgctcggaa gcgtcggaga agagcggggc cgtgctgcgt acggtgagaa acgcgctgcc
     2641 ctgcgacgct ccggtgggcg ccgcgggcgg gccggccagg gccccgtgtg tgaaggtgcc
     2701 caccggagcc gaaacagccg agactccctg cacaagagaa aggctcgccg cgtagcgggt
     2761 gaattcggcg tccgacagcc cgtcgctgtc ggggatgacg acgctgacgt cggtcgcgga
     2821 gtcgttggcg aagtcgctgc gcagctggtc gccgacctgc cgggccgacg cggagtccgg
     2881 cagcacccgg tcgtcgggga agccccaccg cacaccgagg aacggcgccc ccaacaacag
     2941 cagcacggcg accaccgcca ccccgaccgg aactgcgcga cgggtgacga aattcgttga
     3001 gcggtaccag aactcgtcct cgactggctt ccgcgccggc cgcgg
//
LOCUS       DASSGQ010000089         4140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_371229,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000089 DASSGQ010000000
VERSION     DASSGQ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371229"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VFI55_04035"
     CDS             <1..433
                     /locus_tag="VFI55_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7073239.1"
                     /translation="VDAVANSIKNVLGIDAVGTPQPTFAGFRTDITNHTIATAFRAAW
                     QGDYPSMIEFLAPLFATGAGSNDVGYSNPAFDAALAAAEAAPDLRASDVLANAAQRFL
                     VQDMPVVPLWDNISVVGWSAEVKNVHVTWNGLPDYENIVKG"
     gene            435..1361
                     /locus_tag="VFI55_04040"
     CDS             435..1361
                     /locus_tag="VFI55_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7073240.1"
                     /translation="MGWYIVRRIAVMVPVFLGATLLIYGMVFLLPGDPVAAMAGDRPL
                     TPAVAAALRARYHLDDPFMVQYLRYLGGVLHGDLGRSFSGLPVSAVLAHAFPVTIRLA
                     LIAVAVEAVLGIGFGVIAGLRAGGLFDSTVLITGLIIVAIPIFVLGFLAQFVFGVRLG
                     IAPVTVGEHATFGRLLLPGIVLGSVSFAYVVRLTRSAVAANAHADYVRTATAKGLSRP
                     RVVTVHILRNSLIPVVTFLGADLGSLMGGAVVTEGIFNIHGVGGVLFQAVTRQEAPTV
                     VSIVTVLVLVYLVTNLAVDLLYAALDPRIRYG"
     gene            1354..2229
                     /locus_tag="VFI55_04045"
     CDS             1354..2229
                     /locus_tag="VFI55_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7073241.1"
                     /translation="MAERIPDQHGFWWDTWRKLRRRPKFVVASALILLIVVVALFPAW
                     FTGIDPTYADPGQSLLPPSAAHWFGTDLQGHDIYARTIYGARASVTVGLGATLLVFVV
                     GGSFGALAGFYGGWVDAVVSRITDIFFGLPLLLAAIVLMGVMHHRRVWTVIVILAVFG
                     WPQIARIARSAVIEARAGDYVLAAKALGLSNFQTLVRHALPNALGPVFAVATLAFGVF
                     IVTEASLSYLGVGLPTSVVSWGGDINLAQSRLRSGSPILFYPAGALAITVLAFMMMGD
                     ALRDALDPASRALRA"
     gene            2226..3860
                     /locus_tag="VFI55_04050"
     CDS             2226..3860
                     /locus_tag="VFI55_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7073242.1"
                     /translation="MSSPEAPLLSVDALEVRFGDHAPSVCGVDLTVRRGQTVAVVGES
                     GSGKSTTAAAILGLLPTGGRITAGHIVFDGRDITAADRRTLRSIRGRGIGYVPQDPMT
                     NLNPVWKVGFQIREALRANTDNRETRRRALQLLAEAGMPNPVQQAGRYPHQLSGGMCQ
                     RALIAIGLAGRPKLLIADEPTSALDVTVQRQVLDHLQQLTDQLGTALLLITHDLALAA
                     ERAESVVVIHRGVVVESGAAQAILQDPQHEYTRRLVAAAPSLTARSSARTRSRTADEP
                     SDDILVASELTKVYRESHGAPWRRAEFRAVDAVSFRVRRASTLAIAGESGSGKSTVAR
                     MVLGLLQTTSGSVVFDGTPIAPRMDRNQALAFRRRVQPVFQNPYSSLDPMYSVFRAIE
                     EPLRIHRVGDRKQREQAVRELVDHVALPSSVLGRLPRELSGGQRQRVAIARALALRPE
                     VLVCDEAVSALDVLVQAQILDLLAELQNELGLTYLFISHDLAVIRQIADDVLVMRAGR
                     VVEQAPTEELFTRPRHDYTRQLLEAIPRAPAPPQIG"
     gene            3867..>4140
                     /locus_tag="VFI55_04055"
     CDS             3867..>4140
                     /locus_tag="VFI55_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET7073243.1"
                     /translation="MTADPLAPLMKLPGVAEASDAARDALGRAHRHRANLRGWPLTAA
                     EAALRAARASSVLDGGPVRLDDLAEAALPKNPVFGGALRVSQALEGG"
BASE COUNT          591 a         1349 c         1431 g          769 t
ORIGIN      
        1 ggtggacgcg gtggccaaca gcatcaagaa cgtgttgggc atcgacgcgg tgggcacgcc
       61 gcagcctacc ttcgccggat tccgcaccga catcaccaac cacacgatcg cgaccgcgtt
      121 ccgcgcggcc tggcagggag attatccgtc catgatcgag tttcttgccc cgctgttcgc
      181 cacgggggcc gggtccaacg acgtcggcta ctccaacccg gcgttcgacg cggcgctggc
      241 ggccgccgag gcagcacccg acttgcgggc atcggatgtc ctggccaacg ccgcgcagcg
      301 attcctggtg caggacatgc ccgtcgttcc gctttgggac aacatcagcg tggtgggctg
      361 gtcggcagag gtcaaaaacg tccacgtcac gtggaatggc ctgcccgact acgagaacat
      421 cgtgaagggc tgagatgggc tggtacatcg ttcgccgaat cgccgtcatg gtgccggttt
      481 tcctgggcgc gacgctgctg atctacggca tggtgttcct gctgcccggt gatccggtgg
      541 cggcgatggc gggcgaccgc ccgctgacgc cggcggtggc cgccgcgctg cgtgcccggt
      601 accacctcga cgacccgttc atggtgcagt atctgcgcta tctgggcggc gtcctgcacg
      661 gagacctggg acgatcattt tccgggttgc cggtcagtgc cgttctggct catgcgtttc
      721 cggtcaccat caggctggcc ctgatcgcgg tggcggtgga agccgtgctg gggatcgggt
      781 tcggcgtgat cgccggtctg cgcgcaggcg ggctgttcga ttccacggtg ctgatcaccg
      841 gcctcatcat cgtcgcgatc ccgatattcg tgctgggttt ccttgcgcaa ttcgtgttcg
      901 gcgtccggtt ggggatcgcg cccgtcacgg tgggcgaaca tgcgaccttc gggcggctgc
      961 tgctgcccgg gatcgtcttg ggttcggtgt cattcgcgta cgtggtgcga ttgacccgat
     1021 ccgcggtggc cgccaatgcg cacgccgact atgtccgcac cgcaaccgcc aagggactgt
     1081 cgcgaccgcg cgtggtgacc gttcacatat tgcgtaactc gttgattccg gtggtgacct
     1141 tcctcggcgc cgatttgggc tcgctgatgg ggggagccgt cgtgaccgag ggcatcttca
     1201 acatccatgg tgtcggtggc gtgttgtttc aggccgtcac caggcaggaa gcgccgacgg
     1261 tagtctccat cgtgacggtg ctggtgctgg tgtatctggt aaccaatctg gcggtggact
     1321 tgctgtacgc cgccctcgac cctcggatcc gatatggctg aacgcattcc ggaccaacat
     1381 ggcttctggt gggacacgtg gcgcaagttg cgccggcggc cgaagttcgt cgtcgcgtcc
     1441 gcgttgattc tcttgatagt ggtggttgca ctatttccgg cgtggttcac cggaatcgat
     1501 cccacctacg ccgatcccgg ccagagcctg cttcccccgt cggccgcgca ctggttcggg
     1561 accgatctgc agggacacga catctacgcg cgcaccatct acggagcgcg ggcatcggtg
     1621 acggtggggc tgggcgcaac cctgctcgtc ttcgtcgtgg gcggatcgtt cggcgcgctg
     1681 gcgggcttct acggcggctg ggtcgacgcg gtggtctcgc gtatcaccga catcttcttc
     1741 gggttgcccc tgctgctcgc cgccatcgtg ctgatgggcg tcatgcacca ccgcagggtg
     1801 tggacggtga tcgtcatcct ggccgtgttc ggctggccgc agatagccag gatcgcgcga
     1861 agcgcggtga tagaggcgcg agccggtgac tatgtgctcg cggcgaaagc gttggggctg
     1921 agcaacttcc aaaccctggt tcggcatgcc ctgcccaacg ccctgggccc ggtgttcgcg
     1981 gtggccaccc tcgccttcgg tgtcttcatc gttaccgaag cctcgttgtc ctacctgggc
     2041 gtcggtctgc cgacatcggt ggtgtcatgg ggcggcgaca tcaacctcgc gcagagccgg
     2101 ctccggtccg gctcgccgat tctgttctat cccgccggcg cactggcgat tacggtgctg
     2161 gctttcatga tgatggggga tgctttgcgg gacgcgctgg atcctgcgtc gcgggcgttg
     2221 cgcgcatgag ctccccggag gcgccgctgt tgtcggtcga cgctctggaa gtcaggttcg
     2281 gcgaccacgc tccgtcggtt tgcggagtgg atctgaccgt gcggcgcggt cagaccgttg
     2341 cggtggtggg cgaatcggga tcggggaaat ccaccaccgc cgccgccatt ctcgggctcc
     2401 tgccgaccgg agggcgaatc accgccgggc acatcgtttt cgacggccgc gacatcaccg
     2461 ctgccgaccg ccgcacactc cgatcgatcc gtgggcgcgg catcggctat gtgccacagg
     2521 acccgatgac caacctcaac ccggtctgga aggtgggctt tcagatccgg gaagcgctgc
     2581 gcgcgaacac cgacaatcgc gagacgcggc gtcgtgccct gcagctgctg gccgaggcgg
     2641 ggatgccgaa tccggtgcaa caagccggcc ggtacccgca tcagctgtcc gggggcatgt
     2701 gtcaacgggc gctgatcgcc atcggattgg cgggacgacc gaagctcctc attgccgacg
     2761 agccgacgtc cgcgctggac gtcaccgtgc agcggcaggt gctcgaccat ttacagcaac
     2821 tcaccgacca actcggaacg gccctgctac tgatcaccca cgacctggcg ctggccgccg
     2881 aacgggcgga gtccgtcgtc gtcatccatc gcggcgtggt ggtggaatcc ggcgcggcgc
     2941 aagctatcct gcaggacccg caacacgagt acacgcgacg gttggtggcc gctgccccgt
     3001 cgctcacggc gaggagttcg gccagaaccc gatcgcggac ggcggacgag ccctccgacg
     3061 acatcctcgt cgcctcggag ctgaccaagg tctaccggga atcgcacggc gcgccgtggc
     3121 gacgggcgga gttccgcgcc gtcgacgcgg tgtcgtttcg ggtgcggcgg gccagcaccc
     3181 tcgcgatcgc cggcgagtcg ggttcgggca aatcgaccgt ggcgcggatg gtgctgggtc
     3241 tgctgcaaac cacttccggc agtgtggttt tcgacggcac gccgatcgcg ccccgaatgg
     3301 accggaacca ggcgttggct ttccgccgtc gcgtccagcc ggtgttccaa aacccttaca
     3361 gcagtttaga tcccatgtac tcggtgtttc gtgccatcga ggagccgttg cggatccatc
     3421 gcgtcggtga ccgcaagcag cgcgagcagg cggtgcgcga gcttgtcgac cacgtagcgc
     3481 tgccgtcgtc ggtgttgggg cgactgcccc gggagctgtc gggtggtcag cggcaacggg
     3541 tggcgatcgc ccgggcgctg gcgctgcggc ccgaggtgct ggtgtgcgac gaggcggtgt
     3601 cggccctcga cgttctggtg caggcgcaga tattggacct gctggccgag ctgcagaacg
     3661 aactcgggct gacctacctg ttcatcagcc atgacttggc ggtgatccgc cagatcgcgg
     3721 acgacgtcct ggtgatgcgc gccggacgcg tggtggagca ggcgcccacc gaggagctgt
     3781 tcacccggcc ccgccacgac tacacccgcc agctactgga ggccattccg cgtgcccccg
     3841 cacctccgca gataggttga caacccgtga cggctgaccc gctggccccg ctcatgaagc
     3901 tccccggcgt cgccgaggcc agcgacgcgg cccgcgacgc gctgggtcgc gcccaccgcc
     3961 accgggccaa ccttcgcggc tggccgctga ccgccgccga ggcggcgttg cgggcggcac
     4021 gcgcctcgtc ggtgctcgat ggcggcccgg tgcgactgga tgacctggct gaggccgcct
     4081 tgccgaagaa tccggtattc ggcggagcgc tgcgggtgtc ccaggcgctc gagggcggag
//
LOCUS       DASSGQ010000090         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_371258,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000090 DASSGQ010000000
VERSION     DASSGQ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371258"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /gene="rocD"
                     /locus_tag="VFI55_04060"
     CDS             complement(<1..1085)
                     /gene="rocD"
                     /locus_tag="VFI55_04060"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HET7073244.1"
                     /translation="MTMLDARTSEWVAANRTENAIALVERYAAHNYSPLPVVAASAKG
                     AWITDVSGRRYLDCLAAYSAVNFGHGNPEIIATAHAQLDRVTLVSRAFHCDNLGPFCA
                     ALARLCGKDMVLPMNSGAEAVESALKVARKWGADVKGVPAGQGNIIVADNNFHGRTIS
                     IVSFSSDPAARDGFGPFTPGFRSVPFGDAAALAEAIDDDTVAVLLEPIQGEAGIIVPP
                     DDYLPTVRELCSERNVLMIADEIQSGLARTGYTFACERWGVVPDVYVLGKALGGGVVA
                     LSAVVADGDILGVLHPGEHGSTFGGNPLAAAIGTTVVGMLARGEFQARSARLGVHLHR
                     RLRELVGDGVVAVRGLGLWAGVDIDPTL"
     gene            complement(1082..1945)
                     /locus_tag="VFI55_04065"
     CDS             complement(1082..1945)
                     /locus_tag="VFI55_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylarginine dimethylaminohydrolase family
                     protein"
                     /protein_id="HET7073245.1"
                     /translation="MTDTHVPAPALRLAAEPAGRSRTPSTRRYAMTPPAFFAVEYAIN
                     PWMDVSKPIDVHLAQTQWEGLYRTYLQLGHHVDLVEPVRGLPDMVYAANGGIIARDTA
                     IVARFRFAERAGESRAYAAWMTSLGYRPVSTRHVNEGQGDLLMIGEMVLAGYGFRTDR
                     RAHAEISAALGVPVVSLRLVDPRFYHLDTALAVLDDHTVAFYPPAFSAAAQDQLRALF
                     PDAIVVGSADAYVLGLNAVSDGLHVVLPSAATGFATQLRQAGFEPVGVELSELLKGGG
                     SVKCCTLEVFP"
     gene            2066..2512
                     /locus_tag="VFI55_04070"
     CDS             2066..2512
                     /locus_tag="VFI55_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET7073246.1"
                     /translation="MDRLDDTDERILAELMQHARATFAEIGLTVNLSAPAVKRRVDRM
                     LDNGVIKGFTTVVDRNALGWNTEAYVQLFCQGTIAPDQLRAAWVDMPEVVSAATVTGT
                     PDAILHVLARDMRHLEAVLERIRSSADIERSESIVVLSNLIDRMRT"
BASE COUNT          447 a          863 c          856 g          399 t
ORIGIN      
        1 agcgtcgggt cgatgtcgac accggcccac aacccgagac cccggaccgc caccacgccg
       61 tcgccgacca gctcccgcag ccgccgatgc aggtggacgc ccagtctggc cgaacgggcc
      121 tgaaattcgc cgcgtgccag catgcctacc accgtggtac cgatggccgc ggccaacggg
      181 ttcccgccga aggtcgagcc gtgttcgccg ggatgcagta cgccgaggat gtcgccgtcg
      241 gcgaccaccg ccgacagcgc gacgaccccg ccgcccagcg ccttgccgag cacgtacacg
      301 tccgggacga cgccccaccg ctcgcaggcg aacgtgtagc cggtgcgagc cagccccgac
      361 tggatctcgt cggcgatcat cagcacgttg cgctcgctgc acagctcgcg gaccgtcggc
      421 aggtagtcgt cgggcgggac gatgatgccc gcctcgccct ggatgggctc cagcagcacc
      481 gccaccgtgt cgtcgtcgat cgcctcggcc agtgccgcgg cgtcgccgaa cggcaccgag
      541 cggaagcccg gggtgaacgg cccgaagccg tcgcgcgccg cggggtccga cgagaagctg
      601 acgatgctga tcgtgcgccc atgaaagttg ttgtcggcca cgatgatgtt gccctgaccc
      661 gccgggacgc ccttgacgtc ggcgccccac ttgcgggcga ccttgagtgc gctctccacg
      721 gcctcagcgc ccgagttcat cggcagcacc atgtccttgc cgcacaaccg ggcaagcgcg
      781 gcgcagaacg gtccgagatt gtcgcaatgg aatgcgcggc tgaccagcgt gaccctgtcg
      841 agttgcgcgt gagccgtcgc gatgatctcc gggttgccat gaccgaagtt gactgccgag
      901 taggcggcca gacagtcgag gtagcgccgg ccgctgacat cggtgatcca cgcgcccttt
      961 gcgcttgccg cgactacggg tagcggcgaa taattgtgcg ccgcatacct ttcgactagt
     1021 gcgatcgcgt tctcggttcg gttcgcggcg acccattccg aagtccgggc gtcgagcatc
     1081 gtcatgggaa tacctccaac gtgcaacatt tgaccgaccc gccgcccttg agcagctcgg
     1141 acagctcgac gccaaccggc tcgaagccgg cttgacgcag ctgtgtggcg aaacccgttg
     1201 cagcagaggg aagtacaacg tgcagaccgt cggagacggc gttgagacca agcacgtagg
     1261 cgtcggcgct tcccacgacg atagcgtcgg gaaacagcgc acgcagctgg tcttgcgccg
     1321 ccgcgctgaa cgccggcgga tagaacgcaa cggtgtggtc gtcgagaacg gccagcgcgg
     1381 tgtccaggtg atagaagcgc gggtccacca gccgcagcga gacgaccggc actcccagcg
     1441 ccgcggagat ttcggcgtgc gcgcgccggt cggtgcgaaa gccgtagccc gctaacacca
     1501 tttcgccgat catcagtagg tcgccctgtc cctcgttgac gtggcgagtg gacaccggcc
     1561 ggtagcccag cgacgtcatc cacgctgcgt aggcccgaga ctctcccgcc cgttcggcga
     1621 accggaacct ggccacgatg gcggtgtcgc gggcgatgat cccgccgttg gccgcataga
     1681 ccatgtccgg aagcccacgc accggctcga ccaggtcgac gtggtgaccc agctgcaggt
     1741 aggtccggta caggccctcc cactgcgttt gcgcgaggtg gacgtcgatg ggcttgctga
     1801 cgtccatcca cgggttgatc gcgtactcga ccgcgaagaa agccggcggg gtcatcgcgt
     1861 accggcgagt actgggtgtg cggctgcgcc cggcgggctc ggccgcgagc cgtaacgcgg
     1921 gtgcggggac gtgagtgtcc gtcataaacc aaccatattt tggcgctgct aaacaatcaa
     1981 acgacgatta ttgcgtgcct atagccgatt tattgcgcta aagtcgctgg tgcagcgatt
     2041 tgttgcatac gaatggggag cagtgatgga ccgcctggat gacaccgatg agcgcatcct
     2101 cgccgagctg atgcagcacg cgcgggcgac gttcgccgaa atcgggctga cggtgaatct
     2161 gtccgcgccg gcggtgaagc gacgggtcga ccggatgctc gacaacggcg tgatcaaggg
     2221 cttcaccacg gtcgtcgacc gcaacgcgct cggctggaac accgaggcct atgtgcagct
     2281 gttctgccag ggcacgatcg ctcccgatca gctgcgggcg gcctgggtgg acatgcccga
     2341 ggtggtcagc gcggcgacgg tgacgggcac gcccgacgcg atcctgcacg tgctggcccg
     2401 cgacatgcgg cacctggagg cggtcctcga gcgcatccgg tccagcgccg acatcgaacg
     2461 cagcgaaagc atcgtcgtgc tgtccaacct catcgaccga atgcggacct agcggccttt
     2521 gggccgccca acccgccgcg cgtgcgtaac gccactgcga gagtc
//
LOCUS       DASSGQ010000091         3685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_674938,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000091 DASSGQ010000000
VERSION     DASSGQ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674938"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VFI55_04075"
     CDS             complement(<1..698)
                     /locus_tag="VFI55_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073247.1"
                     /translation="MGMLFGFAPWIVYWVLVGNVPFAVAALIALAFAVAAYAIGAATG
                     KPGHPLETGAVATFLVLTILTFTLSDAFSQRWTLPLSNAGIFLVALASVLMGKPFVHE
                     FAAAEQTDDVVKTGLFGRIVRLLAWIWVAAFAGMTVSSAVAPTLEGRTGIAASILDTK
                     TPLSFLCYWVIPFSLLGLAVLASRLLPDRMLIGIDDLARETSFVAYDEATIDELYFLA
                     QEHANREVGPGKEA"
     gene            complement(797..870)
                     /locus_tag="VFI55_04080"
     tRNA            complement(797..870)
                     /locus_tag="VFI55_04080"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(834..836),aa:Leu,seq:taa)
     gene            1230..2090
                     /locus_tag="VFI55_04085"
     CDS             1230..2090
                     /locus_tag="VFI55_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015627097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE domain-containing protein"
                     /protein_id="HET7073248.1"
                     /translation="MSFLTTIPEELLAAAAQLGGIGSSLSAQNAGAAAPTTAVAPAAS
                     DQVSTLQAGIFSAYGTLYQQIAAEAQTVQEQFVSTLGLSSGTYGDTEAANASQSALAG
                     PLQNLTGLLNNGAILLGGPGNSVGGNPFTLSGNSANFGSFEFGNWASAMSDCLGMAGG
                     GLLPAAPADAGADAAAAAGAADSVNAVGPAGSGVGAMGAMPAASVGQATMVGKLSAPP
                     SWAAGTFTPGASPATAIQTVGWTGAAPQAGPGTIIPGMPGMGAARNSAGFGAPRYGVK
                     PIVMPKPTAV"
     gene            2166..3401
                     /locus_tag="VFI55_04090"
     CDS             2166..3401
                     /locus_tag="VFI55_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPE family protein"
                     /protein_id="HET7073249.1"
                     /translation="MVLDFAALPPEVNSALMYSGSGSGPMMAAATAWSNLAAELSTTA
                     TSWESIVASLTSEQWTGLGSAAAAAASAPYVNWLSTTAAAAEQASAQAASSAAAYEAA
                     FTGTVPPPLIAANRAQLAALVATNFLGINTPAIMATEALYAEMWVQDATTMYTYAAAA
                     TAASALQPLTPASPTASPAAPATQAAAVGAAAAATPAQGGLGSLISGLQGALGSLGTP
                     AAVQPIGTGVWTALQTSYPNLANLIFAADGLGGTLFNFNGVQQIGVTAAWWVGNTIPT
                     AVSLGHTLAASAPAAAASDVTPAAGGAIIGEGSLASSVAPGGGLGGAATAGIGEAGSV
                     GGLSVPAGWSAAAPATLASSTAPLDGSGWTAAADEAAPVGAMPGMPGMAAAAKGAGAY
                     GAGPRYGFKPIVMPKQVVV"
     gene            3547..>3685
                     /locus_tag="VFI55_04095"
     CDS             3547..>3685
                     /locus_tag="VFI55_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HET7073250.1"
                     /translation="MARFMTDPHEMRAMAGRFEMHAQTVEDEARKMWASSMNIAGAGW
                     SG"
BASE COUNT          642 a         1236 c         1240 g          567 t
ORIGIN      
        1 taggcctctt tgccggggcc gacctcgcga ttggcgtgtt cctgggccag gaagtacagc
       61 tcgtcgatgg tggcttcgtc gtaggcgacg aatgaggtct cccgggcgag gtcgtcgatg
      121 ccgatgagca tccggtcagg cagcaatcgc gacgccagca ccgccagccc caacagcgag
      181 aagggaatga cccagtaaca caggaatgac agcggagtct tggtgtccag gatgctcgct
      241 gctattccag tccggccttc caacgtcggc gcgaccgccg acgacaccgt catgccggcg
      301 aacgcggcga cccagatcca ggccaacaac cggacgatcc ggccgaacaa tcccgtcttg
      361 accacatcat cggtttgttc cgcggcggcg aactcatgca cgaacggctt gcccatcagc
      421 acgctcgcca gcgcgaccag gaagatcccc gcgttgctca gcggcagtgt ccaccgctga
      481 ctgaacgcgt cgctcagcgt gaatgtcagg atcgtgagca ccaggaacgt cgcgacggcc
      541 ccggtttcga gcggatgccc cggtttgccg gtggcggcgc cgatcgcata cgcggcgacg
      601 gcgaaagcca gcgcgatcag cgcggcgacg gcgaacggga cgttgccaac cagcacccaa
      661 tagacgatcc acggcgcaaa gccgaacagc atgcccacaa tcagccagtt tatgagtggc
      721 ggttcgcgac gccgtcggcg gcaagcgcca acgtggcttg ggacccagaa atggactgct
      781 accaggaatt taccggtgcc cccagtcgga ctcgaaccga cactggacgg attttaagtc
      841 cgctgcctct gccaattggg ctatgggggc ccggcggcga atctagcgcg ccaagcgggc
      901 atccggggca ccgcatccgc cgaaaaaata gcccgattcc gaagggtgcg cggaagtcat
      961 acttcgcgta ggatccgcgc gccgcgcgca gtcctattgg tttgccccca ccgctcaggt
     1021 gacaccgcag tgtcgcccgg caggagcttg ttaaaactgt ccgcgcccac gttaagaagc
     1081 cgtaaacggg ggccacccta gccttctccg tcgcgactgt ggagtcgtgg ctcgttagcc
     1141 ttacgccaac gcacagggcc cgttacgcca acacacaggg tcgatccgcc gcaagcggct
     1201 cggcacgttc cggatactag gagcaataga tgtcatttct gacaacaatt cctgaagagt
     1261 tgctcgccgc agcggcgcag ctggggggga tcggcagttc actgtccgct cagaatgccg
     1321 gtgccgcggc accgaccacg gccgtcgctc ccgcagcatc cgaccaggtg tcgaccctgc
     1381 aggccggaat cttctcggcg tacggcaccc tctaccagca gatcgccgcc gaagctcaga
     1441 ccgttcaaga acagtttgtg agcaccctgg gcctcagctc cggcacctat ggcgacaccg
     1501 aggccgctaa cgcgagccag tccgcgctgg ccggccccct ccaaaacctc accggcctcc
     1561 tcaacaacgg cgcaattttg cttggtggtc cgggcaacag cgtgggcggc aacccattca
     1621 ccctgtcggg taactcggcc aacttcggca gtttcgagtt cggcaactgg gcctcggcta
     1681 tgtccgactg cctcggcatg gccggcggcg gtttgctgcc ggccgcgccg gctgacgccg
     1741 gcgccgacgc cgccgcggca gccggcgcgg cggattcggt caacgcggtc gggccggccg
     1801 gctccggcgt cggggccatg ggcgcgatgc cggccgcgag cgtgggccag gcaaccatgg
     1861 tcggcaagct gtccgctccg cccagctggg cggccggtac cttcaccccg ggcgcgagcc
     1921 ctgccaccgc gatccagacc gtgggctgga ccggggcggc gccgcaggcc gggccgggaa
     1981 cgatcatccc cgggatgccc ggaatgggcg cggcgcgcaa cagcgccggc ttcggtgccc
     2041 cccgctacgg cgtcaagccc atcgtcatgc ccaagccgac ggccgtctag gggactgggc
     2101 tcccacacgg tcgcaggtag tcaaccaaca gcgaaattcg gtcaaaagga acgggaaaca
     2161 gaaaaatggt cttggacttt gcagctttgc ccccggaggt caactccgcg ctcatgtact
     2221 ccggttcggg ctctggaccg atgatggccg ccgcgacggc ctggagcaac ctggccgccg
     2281 aactgagcac caccgcgacc tcctgggagt cgatcgtcgc atcgctgacc agcgagcagt
     2341 ggacgggttt ggggtcggcg gccgcggcgg cggcatccgc gccctacgtg aactggctga
     2401 gcaccacggc ggcggcagcc gagcaggcgt cggctcaggc cgcgtcgtcg gcggccgcgt
     2461 atgaggcggc gtttacggga acggtgcctc cgccgctgat cgctgccaac cgtgctcagc
     2521 tcgcggcgct ggtggcgacg aacttcctgg gcatcaacac gcccgcgatc atggccaccg
     2581 aggccctgta cgccgagatg tgggtgcagg acgccaccac gatgtacacg tatgcggccg
     2641 cggcgacggc ggccagtgcc cttcagccgc tgaccccggc gtcgccgacc gcgagcccgg
     2701 ccgccccggc cacccaagcg gccgcggtcg gcgcggcagc ggcggccacg cccgcccagg
     2761 gcggactggg ttccctcatc tccggcctgc agggcgcttt ggggtcgctt ggcacccccg
     2821 ccgcggtgca gccgatcggt accggtgtgt ggaccgcgct gcaaacgagc taccccaatt
     2881 tggcgaatct gatttttgcc gctgacggcc tcgggggcac cctgttcaac ttcaacggcg
     2941 tccagcagat cggcgtcacc gcggcttggt gggtcggtaa caccatcccg accgcggtat
     3001 cgctggggca cacccttgcc gcctccgcac cggcagcggc cgccagcgac gttactcccg
     3061 ccgcgggcgg ggcgatcatc ggcgaagggt cgctggccag ttcggtggcg ccaggcggcg
     3121 gattgggcgg ggcagcgacg gcgggtatcg gcgaagcggg ctcggtcggc ggtctgtcgg
     3181 tgccggccgg ctggtcggcg gcggccccgg cgacgctggc ttcgtccacc gctccgctgg
     3241 atggctcggg ttggaccgcc gccgccgacg aagccgcgcc tgtcggggcg atgcccggga
     3301 tgccgggaat ggccgcggcg gccaagggtg ccggcgccta cggtgccggg ccgcggtacg
     3361 gattcaagcc aatcgtcatg cccaaacagg tggtcgtctg atggggcgaa aaagggggga
     3421 tatgtcaccc aattgaggcg agcatttaac cggtaggccg taatctgtcc gttatgcggc
     3481 caccaccgca tcaaaatcaa caagacttcg acaaactgaa ggcttagaac aagaaagaag
     3541 ggcaacatgg cacgttttat gactgatccg cacgagatgc gggcgatggc tggtcgtttt
     3601 gagatgcacg cgcagacggt tgaggatgag gcccgcaaga tgtgggcgtc gtcgatgaac
     3661 attgcgggtg cggggtggag tggtc
//
LOCUS       DASSGQ010000092        18191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_6523, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000092 DASSGQ010000000
VERSION     DASSGQ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18191
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6523"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFI55_04100"
     CDS             <1..330
                     /locus_tag="VFI55_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HET7073251.1"
                     /translation="EVAPFGVHVTLIEPAGFDTDWGGASAKHAAPLADYADVHAAADK
                     ERRGRATKQGDHTASAAALLKVVDAEEPPLRVFFGELPLQLAKADYESRLRSWEQWQP
                     VAELAQG"
     gene            complement(354..1508)
                     /locus_tag="VFI55_04105"
     CDS             complement(354..1508)
                     /locus_tag="VFI55_04105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET7073252.1"
                     /translation="MRVAIVAESFLPNVNGVSNSVLRVLEHLRRTGHEALVIAPDNPP
                     RQPRADRIHDGIRVHRVPSRMFPKITTLPLGVPMPRMVKVLRGFDPDVVHLASPALLG
                     YGGVLAARRLGVPTVAVYQTDVPGFAASYGIPMTERAAWAWFRHLHNLADRTLAPSSV
                     TMESLVSHRFPRVHRWARGVDVLRYAPSARDEEVRQRWSPDGKPIVGFVGRLAPEKHA
                     ERLAALAGNDSVQLVIVGDGVDQRKLQSAMPTAVFTGALYGEELATAYASMDVFVHTG
                     EHETFCQVVQEAMASGLPVIAPDAGGPRDLIAPYRTGLLLPVKEFEARLPDAVEHLLA
                     ERHRYALAARRSVLGRSWPVICDELLGHYEAVLSPFARRRMRANRFAQGE"
     gene            complement(1599..2126)
                     /locus_tag="VFI55_04110"
     CDS             complement(1599..2126)
                     /locus_tag="VFI55_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HET7073253.1"
                     /translation="MISAKELLHILIHGRTDEPPTTRPGIVLRWARIAVLIVTALVTL
                     QSALLVAGAWRNDLAIEHNMGVAQAEVLSAGPRRSTIEFVTPERVTYRPELGVLYPSE
                     LATGMRIYVEYNKNDPNLVRVQHRNARLAIIPAGSIAVVSWLVAAASLIGLALLDNWL
                     DRRAASDGRSRQLAS"
     gene            complement(2134..3795)
                     /gene="menD"
                     /locus_tag="VFI55_04115"
     CDS             complement(2134..3795)
                     /gene="menD"
                     /locus_tag="VFI55_04115"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HET7073254.1"
                     /translation="MNPSTTQAHVVVDELIRGGVRDVVLCPGSRNAPLAFALQDADRS
                     GRIRLHVRIDERTAGYLAIGLAIAADAPVCVAMTSGTAVANLGPAVVEANYARVPLIV
                     LSANRPYELLGTGANQTMEQLGYFGTQVRATINLGLAEDAPERLDAFNATWRSATCRV
                     LAAATGSRSANAGPVHFDIPLREPLVPDREPHGAVTPQGRADGRPWTYTPPATFDQPL
                     DIDLSPDTVVIAGHGAGAQPNLAQLPTVAEPTAPAPANPLHPLALPLLHPKQVIMLGR
                     PTLHRPVSALLADPKVPVYALTTGPRWPDVSGNSQATGTRAVTTGAPHPAWLHRCAEM
                     NRHAIAAVRGQLKAHPLTTGLHVAAAVADALRPGDQLVLGASNPVRDAALVGLSSADI
                     RVRSNRGVAGIDGTVSTAIGAALAFEGALERAGSPDSPARTVALIGDLTFVHDSSGLL
                     IGPTEPTPRRLTIVVSNDNGGGIFELLEQGDPRFSDVSSRIFGTPHDVDVGALCRAYH
                     VESRQIEVDELESALDEPGPGMRVLEVKADRSSLRQLHAAIKAAL"
     gene            complement(3868..4650)
                     /locus_tag="VFI55_04120"
     CDS             complement(3868..4650)
                     /locus_tag="VFI55_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7073255.1"
                     /translation="MINLAYDDQGSGEPVVFIAGRGGAGRTWHPHQVPAFLAAGYRVI
                     TFDNRGVGATENAEGFTAQTMVADTAALIESLGAAPARIVGVSMGSFIAQELMVSRPE
                     LVSAAVLMATRGRLDYARKFFHDAEAELYASGVQLPPSYDVRARLLESFSRKTINDDA
                     AVSDWIALFSMWPVKQTPGLRCQLDVAPQTNRLPSYRSIAAPVLVIGFSDDVVTPPYL
                     GREVADALPHGRYLQIPDAGHLGFFERPQAVNAAALQFFGKA"
     gene            complement(4647..5618)
                     /locus_tag="VFI55_04125"
     CDS             complement(4647..5618)
                     /locus_tag="VFI55_04125"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HET7073256.1"
                     /translation="MIPALADLLDRLHVVALPMRVRFRGITTREVALIEGPAGWGEFG
                     AFVEYQAVEASAWLASGIEGAYCEPPPALRDRVPINATVPAVPAAQVGDVLARFPGAR
                     TAKVKVAEPGQTLADDVGRVNAVRRLVPNVRVDANGGWSVEEAVRAAAALTADGPLEY
                     LEQPCTTVDELAALRRRIDVPIAADESIRKADDPLAVVRARAADIAVLKVAPLGGVST
                     LLDIAAQIDMPVVISSAIDSAVGIAAGLSAAAALPRLRHACGLGTGRLFLEDVADVAP
                     PVDGFLPVGPVTPDAARLRALAAPPERRQWWIDRVKACYPLLVPSCG"
     gene            complement(5615..7228)
                     /locus_tag="VFI55_04130"
     CDS             complement(5615..7228)
                     /locus_tag="VFI55_04130"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET7073257.1"
                     /translation="MAQADVVITGTVLTVDDAQPTAEALAVADGRIVAVGSRADVADF
                     IGADTQTVDVGDLNGGCVMPGFVEAHGHPLMEAIALSDRIIDIRPVTLRDADDVVAAV
                     GTEVRKRGADGAYLTGWDPLLQNGLPEPTRAWLDEMAPDGPLVIIHNSGHKAYFNSHA
                     AERLGLTRDTPDPKGAKYGRDSSGELDGTAEETGAVFPLLGGAIQPADYPAMLRAECA
                     RLNRAGLTTCSEMAFDPAFRPLVEQMHDELTVRLRTYEISNPQMSTDATPGEGDDMVR
                     QVGIKIWVDGSPWVGNIALSFPYLDTAATRAIGVQPGSCGHANYTREQLTEIVGAYFP
                     LGWPMACHVQGDAGIDTILGVYEEALRRNPRNDHRLRLEHVGAIRPDQLRRAADLGVT
                     CSIFVDQIHYWGDIIVDGLFGPERGSRWMPAGSAVATGMRISLHNDPPVTPEEPLRNI
                     SVAVTRTAPSGRVLAPDERLTVEQAIRAQTIDAAWQLFADDVTGSLQVGKYADMVVLS
                     ADPRTVPPERIADLEVRATYLAGRQVYRQ"
     gene            7406..9007
                     /gene="fadD8"
                     /locus_tag="VFI55_04135"
     CDS             7406..9007
                     /gene="fadD8"
                     /locus_tag="VFI55_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid--CoA ligase FadD8"
                     /protein_id="HET7073258.1"
                     /translation="MSDELLRNPTHNGHLLVGALKRHKNRPVLFLGESTLTGGQLAER
                     ISQYVQAFEALGAGTGVTVGLLSLNRPEVLMIQGASGTQGYRRTALHPLGSLDDHAYV
                     LSDAGASSLIIDPNPMFVERALGLLERVDALKQILTIGPVPDALTDVAVDLSAEAAKY
                     EPKPLVVADLPPDHIGGLTYTGGTTGKPKGVIGTTGNIASMTQIQLSEWEWPEHPRFL
                     MCTPLSHAGAAFFTPTVVKGGEMVVLGKFDPAEVLKTIEEQRITATMLVPSMLYALMD
                     HPDSHTRDLSSLETVYYGASAINPVRLAEAIRRFGPIFAQYYGQSEAPMAVTYLAKGD
                     HDAKRLTSCGRPTLFARVALLGEDGRPVPQGEPGEICVSGPLLAGGYWNLPDETSRTF
                     KDGWLHTGDMAREDEDGFYYIVDRVKDMIVTGGFNVFPREVEDVVAEHAAVAQVCVVG
                     APDEKWGEAVTAVVVLRGDAARDDAAIEAMTAEIQAAVKERKGSVQSPKQVVVVDALP
                     LTGLGKPDKKAVRARFWEGAGRAVG"
     gene            complement(9096..9644)
                     /locus_tag="VFI55_04140"
     CDS             complement(9096..9644)
                     /locus_tag="VFI55_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7073259.1"
                     /translation="MAERIRSDARRNREKLLEVATAAFAAADGRPVSLESIARDAGVG
                     IGTLYRHFPNREALVEAIYRTELAEVAAAAGQLLERHSPKVALRRWMDRYASFVAAKR
                     GMAESLHAMFDSGALAPSQTRESIVGAVDMLLGAGVSDGTLRSEVQADDVVSSLIGIF
                     LTSGSPEQTGRMLDLLVAGVSR"
     gene            9728..10666
                     /locus_tag="VFI55_04145"
     CDS             9728..10666
                     /locus_tag="VFI55_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7073260.1"
                     /translation="MTDKPEPGGVGSIGTNAVARIGYGAMQLFETSPDAAAAVLRRAV
                     ELGVNHIDTASFYGPGEVNRRIRAALTPYPNDLVIVSKVGARYTGEEPVPLAAAQRPA
                     ELRAAVEDDLSQLGVECIPVVNLRRLDVGPGLQAEGDQIVDLDDQLAEMIALRDEGKI
                     GGIGVSSVPLDVLRRALPAGIACVQNAYSLLDRSQEEMLDVCTAEGIAWVPYFPLGSA
                     FPGFPKVADKPVVVDIAGEIGATSSQVGLAWLLAHAPTTLLIPGTRSIVHLEENIGAG
                     NVTLSAGAIARLDAVTTPGADPWAHGVEPFLEAPRT"
     gene            10691..11179
                     /locus_tag="VFI55_04150"
     CDS             10691..11179
                     /locus_tag="VFI55_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET7073261.1"
                     /translation="MANADGLKPPLRAPWWLKPTNKVFIQLSRLGLSFGGESPVVLTV
                     KGRKSGRDRSTPVTPMTVDGKQYVVAGFPGADWVSNVRAANEVTVARGRRVQQLRMVE
                     LSAEDSRPFLRAFPTQVPTGVGFMKRSGLIKEGRPDEFEALAGVCPVFRLDPVQDGVQ
                     KP"
     gene            11176..12078
                     /locus_tag="VFI55_04155"
     CDS             11176..12078
                     /locus_tag="VFI55_04155"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HET7073262.1"
                     /translation="MSDNPFDAQAWRPVDGFGDLTDITYHRHVHDATVRVAFNRPEVR
                     NAFRPHTVDELYRTLDHARMSPDVGVVLLTGNGPSPKDGGWAFCSGGDQRIRGRSGYQ
                     YASGETADTVDAARAGRLHILEVQRLIRFMPKVVICLVNGWAAGGGHSLHVICDLTLA
                     SREHARFKQTDADVGSFDGGYGSAYLARQVGQKFAREIFFLGRSYTAEQMHHMGAVNE
                     VVDHADLERVGVQWAAEINAKSPQAQRMLKFAFNLLDDGLVGQQLFAGEATRLAYMTD
                     EAVEGRDAFLEKRDPDWSPFPRYF"
     gene            12181..13077
                     /locus_tag="VFI55_04160"
     CDS             12181..13077
                     /locus_tag="VFI55_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7073263.1"
                     /translation="MSKSPFRRFTEQIALAGMRPPSSPQVLVNRPAIKPVDLDGKRVL
                     LTGASSGIGEAAAEQFARRGATVVVVARRQDLLDALAARITAAGGTAESIPCDVSDMD
                     AVDALVADVEKRIGGVDILINNAGRSIRRPLAESLERWHDVERTMVLNYYAPLRLIRG
                     FAPGMLERGDGHIINVSTWGVLTEASPLFAVYNASKAALSAVSRVVETEWGRKGVHST
                     TLYYPLVATPMIAPTKAFEGMPALTSEEAAEWMITAARTRPVRIAPRMAIAAKALDSF
                     GPRWVNSLMARQSVQPNHEASG"
     gene            13121..13762
                     /locus_tag="VFI55_04165"
     CDS             13121..13762
                     /locus_tag="VFI55_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HET7073264.1"
                     /translation="MSDVWTTISAERGALAGDLADLTPAQWDTPSLCAGWTVRDVVAH
                     LSATASLNPALFFLGMARARFNFDRFANERIARHRGADPVATLAEFRGLQNSTSAPPG
                     PTTSWLGEVVVHGADIRRPLGIPHTYARGAVRQVIDFYKGSNLLIGSKNRIAGLALHA
                     TDHDWRHGQGERDAWVEGPLLSLLLAMTGRAAACDDLIGPGVATLRSRCAPNR"
     gene            13784..14191
                     /locus_tag="VFI55_04170"
     CDS             13784..14191
                     /locus_tag="VFI55_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7073265.1"
                     /translation="MEILASRMLLRPADYERSLSFYRDEIGLAIARDYGAGTVFFAGQ
                     SLLELAGYGSPDHSRGPFPGALWLQVRDIAATQAELQGRGVPIAREARREPWGLHEMH
                     VTDPDGITLIFVEVPPGHPLRRDARQDRGATPG"
     gene            14289..15533
                     /locus_tag="VFI55_04175"
     CDS             14289..15533
                     /locus_tag="VFI55_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HET7073266.1"
                     /translation="MTIDLFLLIIVVITALGFDFTNGFHDTGNAMATSIASGALKPKA
                     AVALSAVLNLVGAFMSTAVAATIAKGLIDGHIVTLELVFAGLVGGIVWNLLTWLFGIP
                     SSSSHALIGGIVGATIAAVGAHGVLWKGLVSKVLVPAVIAAILAIMVGALATWLVYRI
                     TRGVPEQRTEAGFRYGQWGSASLVSLAHGTGDAQKTMGVIFLALMSYGAISKSAAAPP
                     LWVIGACALAMAMGTYLGGWRIIRTLGKGLVEIKSPQGMAAESSSAAVILLSTHFGYA
                     LSTTQVCTGSVLGSGLGKPGGEVRWSVAGRMAVAWVFTLPLAGLVGAITYWIVHEIGG
                     YPGAIIGFGMLVAVASAIYIRSRKVKVDHNNVNAEWEGSLTAGLDASDDHQPPSDTGP
                     KIGTGSPRSGSNDSTVIAGNAS"
     gene            15530..15829
                     /locus_tag="VFI55_04180"
     CDS             15530..15829
                     /locus_tag="VFI55_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073267.1"
                     /translation="MSHLSEWFNYQASLKIFIFALLAGAALPGLFALGLRLHVVGGGQ
                     VSVGGATPHKNPALLALSYAIFALVVAVIVFALLYVSRDFLAHTTGHPFLGAKPK"
     gene            15894..16196
                     /locus_tag="VFI55_04185"
     CDS             15894..16196
                     /locus_tag="VFI55_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HET7073268.1"
                     /translation="MNRFLNSVVSWLRAGYPDGIPPTDTFAVLALLTRRLSNDEVKAV
                     AHEFMQRGDFDDVDIGVAISKLTDELPSPEDIERVQTRLAAKGWPLDEGRDAGDPV"
     gene            16265..17365
                     /gene="menE"
                     /locus_tag="VFI55_04190"
     CDS             16265..17365
                     /gene="menE"
                     /locus_tag="VFI55_04190"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HET7073269.1"
                     /translation="MLVGRDSALVAVPADGDHDPELDALRAGLRVGEEIDDDVALVAV
                     TSGITSTPKGALLTPAALTASASATHERLGGPGSWLLALPPHHIAGVQVLVRSVLAGT
                     VPVELDVSAGFNVAELPGAVRRLGSGRRYASLVATQLAKALADPAAAYALAELDAVLL
                     GGGPAPRPILDAAAAAGITVVRTYGMSETAGGCVYDGVPLDGVLLRVPADGRIQIGGA
                     TLAKGYRNPVDPDPFAEPGWFSTDDLGTVNEAGVLTVLGRVDDAISTGGLTVLPQPVE
                     AALCTHPAVSECAVFGLADDRLGQRVVAAIVVGDGCEAPTLDALRAQVTRTLDATAAP
                     RELHLVAALPRRGIGKVDRTALVRRFSRADDQ"
     gene            17377..>18191
                     /locus_tag="VFI55_04195"
     CDS             17377..>18191
                     /locus_tag="VFI55_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073270.1"
                     /translation="MKVARRESLAVAVGVVLVAAAVLLPWLDRGIVPRLDIGPQPFAT
                     HAQAAPIFGTWEIHASWGTGPAILIALAAVAWGPGVAQRLSWRTLTLGTWATACAWAF
                     ALAMIDGWQRGFAGRLTTRDEYLSQVPTITDIPAALRTFTSRIVDYQPNSWVTHVSGH
                     PPGALLTFVWLDRIGLHGGAWAGLLCLLAGSSAAAAVLIGVRALADEQTARRAAPFIA
                     VAPTAIWIAVSADGYFAGVAAWGIALLAVAVHRPVRFPALVAAAAGLLLGWAV"
BASE COUNT         2833 a         6202 c         6344 g         2812 t
ORIGIN      
        1 gaggtcgccc cgttcggggt gcacgtcacg ctgatcgagc cggccggatt cgacaccgac
       61 tggggcggcg catcggccaa acacgcggcc ccgcttgccg attacgccga tgtgcacgcc
      121 gccgccgaca aagagcgccg cgggcgggcg accaagcaag gcgatcacac ggcctcggcg
      181 gcggcgctgc tcaaggtcgt cgacgcggaa gagccgccgc tgcgggtctt tttcggcgag
      241 ttgccgctgc aactggcgaa ggccgactac gagagccggc tacgcagttg ggagcaatgg
      301 cagccggtcg cggagctggc gcagggctaa cgccacgcgg atctgcttgc ggctcactcg
      361 ccttgcgcga accggttggc gcgcatgcgc ctgcgggcga agggcgacag caccgcctcg
      421 tagtggccga gcagttcgtc gcagatgacc ggccagctgc ggccgagcac gctgcggcgg
      481 gcggcgagcg cgtagcggtg tcgttcggcg agtaggtgct caaccgcgtc gggcagccgc
      541 gcctcgaact ccttgacggg cagcaagagt ccggtccgat acggcgcgat caggtcacgc
      601 gggccgccgg cgtcgggggc gatcaccggc aaccccgacg ccatcgcctc ctgcacgact
      661 tggcaaaacg tctcgtgctc accggtatgc acgaagacgt ccatgctggc atacgcggtc
      721 gcaagctctt cgccgtacag cgcaccggtg aaaaccgctg tgggcattgc cgattgcagt
      781 ttgcgttggt cgacgccgtc gccgacgatg acgagctgca cggagtcgtt gcccgccagt
      841 gcggccagcc gctcggcgtg cttctccggc gcgagccttc ccacgaaccc gacaatcggc
      901 ttgccgtcgg gcgaccagcg ctgccggact tcctcgtcac gcgccgacgg cgcgtaccgt
      961 agcacgtcaa ctccgcgcgc ccaccgatgc acgcgcggaa agcgatgtga gacaagagat
     1021 tccatcgtca cgctggacgg agccagcgtg cggtccgcga ggttgtgcag gtggcggaac
     1081 cacgcccacg ccgcccgttc cgtcatcggg atgccgtagc tcgcagcgaa acccgggaca
     1141 tcggtttggt acaccgcgac cgtcggcacg ccgagccgcc gggccgccag cacccccccg
     1201 tagccaagca gcgccggcga cgccagatgt acgacatcgg gatcgaatcc gcgcagcacc
     1261 ttgaccatcc gcggcatcgg cacaccaagc ggcaacgtgg tgatcttggg gaacatccgc
     1321 gacgggaccc ggtgtacccg gatgccgtcg tgaattcgat cggcccgtgg ttgacgcggc
     1381 gggttgtcgg gtgcgatcac cagagcttcg tggccggttc ggcgcagatg ctctaggaca
     1441 cgcaacactg agttgctgac accgttgaca ttggggagga atgactcggc gacgatggca
     1501 acgcgcacga gaccagcatg tcagcatcgg ctgtcgataa ggttgccgac gggcatacgt
     1561 cgcgcgaaat ctgctggtcg gacggcgttc ggacggcctt aggaagcgag ttgcctcgac
     1621 ctgccgtcgg acgcggcacg ccggtccagc cagttgtcca gcaacgccag gcctatcagg
     1681 ctagccgccg cgaccagcca gctcaccacg gcgatggatc ccgccggaat gatcgccagc
     1741 cgcgcgttgc ggtgctgcac ccggaccagg ttcgggtcgt ttttgttgta ttcgacatag
     1801 atccgcatcc cggttgccaa ttcggacgga tacagcacgc cgagctcggg gcggtaggtg
     1861 acacgctcgg gtgtgacgaa ttcgatggtg gagcgccgtg gaccggcgct gagcacctct
     1921 gcctgcgcga cccccatgtt gtgttcgatc gcaaggtcgt tgcgccaggc accggccacc
     1981 agcagcgccg actgcagcgt gacgagggcc gtcacgatca gcaccgcaat ccgcgcccac
     2041 cgcaacacaa tcccgggccg ggttgtcggc ggttcgtcgg tgcgaccatg aatcaggatg
     2101 tgcagcaact cttttgcaga gatcacggag tgctcatagc gctgccttga tggcggcgtg
     2161 cagctgccgc agcgacgacc gatccgcctt gacctcgagc acccgcatgc ccggccccgg
     2221 ctcgtcgagg gccgactcga gctcgtcgac ctcgatctgg cggctctcca cgtggtaggc
     2281 gcggcacagc gcgccgacgt cgacgtcgtg cggggtcccg aagattcgcg acgacacgtc
     2341 ggagaaccgt gggtcgccct gctcgagcag ttcgaagatg ccgccgccgt tgtcgttgga
     2401 caccacgatc gtcaggcggc gcggggtcgg ttcggtgggg ccgatcagca gcccggagct
     2461 gtcgtgcacg aaggtcaggt cgccgatcag cgcgacggtg cgggccgggc tgtcggggct
     2521 gccggcgcgc tcgagggccc cctcgaatgc caacgcggcc ccgatcgcgg tggacacggt
     2581 gccgtcgatg ccggcgactc cgcggttgga ccggacccgg atgtcggcgc tggacaggcc
     2641 caccagcgcc gcgtcgcgga ccgggttgga cgccccgagc accagctggt ctcccggccg
     2701 cagcgcgtcg gccacggccg cggcgacgtg caggccggtg gtgagcgggt gcgccttgag
     2761 ttgaccgcgg accgcggcga tcgcgtgccg gttcatctcc gcgcagcggt gcaaccacgc
     2821 cgggtgcggc gccccggtgg tgaccgcccg ggtgccggtg gcctgggagt tgcccgagac
     2881 gtcgggccag cgcggcccgg tggtcagcgc gtacaccggc accttggggt cggccagcag
     2941 cgccgacacc gggcggtgca acgtcgggcg gccgagcatg atcacctgct tcgggtgcag
     3001 cagcggcagc gccagcgggt gcagggggtt ggccggggcg ggagccgtcg gctcggcgac
     3061 ggtgggcaac tgcgccaggt tgggctgcgc gcccgcgcca tggcccgcga tgacgacggt
     3121 gtccggcgac aggtcgatat ccagcggttg gtcgaacgtg gccggcggcg tgtaggtcca
     3181 aggcctgccg tcggcccggc cctgcggcgt caccgcgccg tgcggctctc gatcgggcac
     3241 caatggctca cgcaggggga tgtcgaagtg cacgggcccg gcattggcgc tgcgagatcc
     3301 cgtggcggcc gccaagaccc ggcaggtggc cgatcgccag gtggcgttga acgcgtccag
     3361 ccgctcgggc gcgtcctcgg ccaggcccag gttgatggtg gcgcggacct gggtgccgaa
     3421 gtagcccagc tgctccatgg tctggttggc cccggtgccc agcagttcgt agggccggtt
     3481 ggccgacagc acgatcagcg gcacccgcgc gtagttggcc tcgaccaccg ccgggccgag
     3541 gttggccacc gcggtgccgg acgtcatcgc gacacatacc ggggcgtcgg cggcgatggc
     3601 cagcccgatg gccaggtaac cggcggtgcg ctcgtcgatg cgaacgtgca accggatccg
     3661 gccggaccgg tcggcgtctt gcagcgcaaa tgccagcggc gcgtttcgcg atcccgggca
     3721 cagcaccaca tcgcggacgc cgccgcggat cagctcgtca acgacgacgt gggcctgagt
     3781 cgtcgacggg ttcacttcta cagggtgtca tacgcggcta accgcgcgcc gaacgtggag
     3841 tcgtcggcta cccgacgtcg ggcgcaacta ggccttgccg aagaactgca gcgccgcggc
     3901 gttgacggcc tgcggccgct cgaagaaccc gagatgaccg gcatcgggta tctgcaggta
     3961 acggccgtgc ggcagcgcgt cggcgacctc tcgtcccagg tacggcggcg tcaccacgtc
     4021 gtcggagaag ccgatcacca gcaccggcgc ggcgatgctg cggtaagacg gcaaccggtt
     4081 ggtctgcgga gcgacatcca gctggcagcg caatcccggg gtctgcttga ccggccacat
     4141 gctgaacaac gcgatccagt cggagacggc cgcgtcgtcg ttgatggtct tgcgcgagaa
     4201 gctttccagc aaacgagccc gcacatcgta ggagggcggg agctggaccc cggaggcata
     4261 cagctcggcc tcggcatcgt gaaagaactt gcgggcgtag tccaggcggc cgcgggtggc
     4321 catcagcacc gcggcgctga ccagctcggg ccgcgacacc atgagttcct gcgcgatgaa
     4381 cgagcccatg gacaccccga cgatccgcgc cggggcggcg cccagcgact cgatcagcgc
     4441 ggcggtgtcg gcgaccatcg tttgcgccgt gaacccttcg gcgttttcgg tggcgccgac
     4501 cccgcggttg tcgaaggtga tcactcggta tccggccgcc aaaaacgccg ggacctggtg
     4561 cggatgccac gtacggccgg cgcccccgcg gccggcgatg aaaacaacgg gctcaccgga
     4621 cccctgatcg tcgtaagcca ggttgatcat ccgcacgacg gtacaagcag cggatagcag
     4681 gccttgaccc ggtcgatcca ccactgccga cgctcaggtg gcgcggccag cgcccgcaac
     4741 cgggccgcgt cgggcgtcac gggcccgacc ggcaggaagc cgtcgaccgg cggggcgacg
     4801 tccgccacat cctccaagaa caaccggccg gtgcccagcc cgcaggcatg ccgaagccgc
     4861 ggcagggcgg ccgcggcgct caacccagcg gcgatcccca ccgccgaatc gatcgcgctg
     4921 gagatcacca ccggcatgtc gatctgtgcg gcgatatcaa gcagggtcga aaccccgccc
     4981 agcggagcaa ctttcagcac cgcgatgtcg gcggcgcggg ctcggaccac ggcgagcggg
     5041 tcgtcggcct tacggatgct ttcgtccgcg gcgatcggca cgtcgatccg ccggcgcagc
     5101 gcggcgagtt cgtcgacggt cgtgcagggt tgctcgaggt actccagcgg gccgtcggcg
     5161 gtcagcgccg cggcggcccg cacggcctcc tcgacgctcc agccgccatt ggcgtccacc
     5221 cgcacgttgg ggaccagtcg acgcaccgcg ttgacccggc cgacgtcgtc ggccagcgtc
     5281 tgcccgggct cggcgacctt caccttggcc gtgcgggcac cggggaaccg ggccagcacg
     5341 tcgcccacct gggccgcggg aaccgcgggc accgtggcgt tgatcgggac acggtcgcgc
     5401 aacgccggcg gcggctcgca gtaggcgcct tcgatgccgg acgccagcca cgcggaggcc
     5461 tcgacggcct ggtattccac gaacgccccg aattcgcccc agcccgccgg gccctcgatc
     5521 aacgcgacct cgcgggtcgt gatgccgcgg aaacgcaccc gcatcggcag cgccaccacg
     5581 tgtagccggt cgagcaggtc cgctagggcc ggtatcactg ccggtagacc tggcggcccg
     5641 ccagatacgt cgcacgcacc tcgagatcgg cgatccgttc cggcggcacc gttcggggat
     5701 cggccgacag caccaccatg tcggcgtact tgcctacctg tagggacccg gtcacgtcgt
     5761 cggcgaacag ctgccaggcg gcgtcgatgg tctgcgcgcg gattgcctgt tcgaccgtca
     5821 atcgctcatc gggtgccagc acccggccgc tgggcgccgt ccgggtgacg gccacgctga
     5881 tattgcgcag cggctcctcg ggggtgaccg gcggatcgtt gtgcagcgag atgcgcatgc
     5941 cggtggccac cgcggatccc gctggcatcc aacgggatcc gcgctcgggt ccaaagaggc
     6001 cgtcgacgat gatgtcgccc cagtagtgga tctggtcgac gaagatgctg caggtgacgc
     6061 cgaggtcggc ggcgcgccgg agttggtccg ggcggatggc gccgacatgt tcgagccgta
     6121 gccggtggtc gttgcgcggg ttgcgacgca gcgcctcttc atacacgccg agaatggtgt
     6181 cgatgccggc gtcgccctgc acgtggcagg ccatgggcca gccgagcgga aagtaggcgc
     6241 cgacgatttc ggtcagctgc tcgcgggtgt agttggcgtg tccgcaggaa ccgggctgca
     6301 ccccgatggc gcgggtggcg gcggtgtcca ggtacggaaa cgacagggcg atgttgccca
     6361 cccacggcga gccgtccacc cagatcttga tgccgacctg gcgcaccatg tcgtcgccct
     6421 cgccgggtgt ggcgtcggtg ctcatctgtg ggtttgagat ctcgtaggtg cgcagccgga
     6481 ccgtcagctc atcgtgcatc tgctcgacca gcggccgaaa cgccgggtcg aaagccattt
     6541 ccgagcaagt ggtcaggccc gcccggttga gccgggcgca ttcggcgcgc agcatcgccg
     6601 ggtaatcggc gggctgaatg gcgccgccca gcagcgggaa caccgcaccg gtttcctcgg
     6661 cggtgccgtc gagttcgccg ctggagtcgc gaccgtattt ggcgcccttc ggatctggtg
     6721 tgtcccgggt caacccgagg cgctcggcgg cgtgcgaatt gaaatacgcc ttgtgtcccg
     6781 agttgtggat gatcaccagc gggccgtcgg gcgccatctc gtcgagccag gcccgcgtcg
     6841 gctccggaag tccgttctgc agcaacggat cccagccggt cagataggcg ccgtccgcac
     6901 ctcgcttgcg aacctcggtg ccgaccgccg cgacaacatc gtccgcgtcg cgcagcgtga
     6961 ccggtcggat gtcgatgatc cggtccgaca gcgcgatggc ctccatcaac ggatggccgt
     7021 gcgcctcaac gaatcccggc atgacacagc ctccattgag atcgccgaca tcgacggtct
     7081 gcgtgtcggc gccgatgaag tcggcgacat cggcgcggct gccgaccgcg acgatccgcc
     7141 cgtcggcgac tgcgagtgct tcggccgtgg gttgcgcgtc atccaccgtc agcacggttc
     7201 cggtgatgac gacatctgcc tgcgccatgt ccagggatcc tagtggcgat cgcgagtgcg
     7261 gcggagccgg acgacgcggg tcgccaccat tcaagctagc ggcgatcgcg agtgcggcgg
     7321 agccggacga cgcgggtcgc cacagtcgac gtagtgaccg agggcggcga cgggcaccaa
     7381 ttgcaacacg ttctagtctt gcgtcatgag cgacgagctg ctgcgcaacc cgacccataa
     7441 cggccacctg ctggtgggtg cgctcaagcg ccacaagaac aggccggtgc tgttcctggg
     7501 cgagtccacg ctgaccggcg gtcaactggc cgagcggatc agccagtacg tgcaggcgtt
     7561 cgaggcgctg ggcgccggca ccggtgtcac ggtcgggctg ctgtcgctca accgtcccga
     7621 agtgctgatg atccagggcg ccagcgggac ccagggctat cggcgcactg cgctgcatcc
     7681 gctgggctcg ctggacgatc acgcctacgt gctctccgac gccggcgcca gttcgctgat
     7741 catcgacccc aaccccatgt tcgttgagcg ggcgctgggg ctgttggagc gggtggacgc
     7801 gctcaagcag atcctgacca tcggcccggt gcccgacgct ttgacagatg tcgcggtcga
     7861 tctgtcggcc gaggccgcca agtacgagcc gaagccgctc gtcgtcgccg acctgccgcc
     7921 ggaccacatc ggcgggctga cctacaccgg tggcaccacc ggcaagccca agggcgtgat
     7981 cggaaccacc ggcaacatcg ccagcatgac gcaaatccag ctgtcggaat gggaatggcc
     8041 cgagcacccg cggttcctga tgtgtacgcc gctgtcgcac gcgggtgcgg cgtttttcac
     8101 acccacggtg gtcaagggcg gcgagatggt ggtgctgggc aagttcgacc cggccgaggt
     8161 gctgaaaacg attgaggagc aacgcatcac ggccaccatg ctggtgccgt cgatgctcta
     8221 cgcgctgatg gaccacccgg attcacacac ccgggacctg tcgtcgctgg aaaccgtcta
     8281 ctacggcgcc tcggcgatca acccggtgcg gctggccgag gccatccgcc ggttcggccc
     8341 gatcttcgcg cagtactacg ggcagtccga ggcgccgatg gcggtgacct atctggccaa
     8401 gggcgaccac gacgcgaagc ggctgacttc gtgcgggcgc ccgacgctgt tcgcgcgcgt
     8461 cgcgctgctg ggggaggacg gcaggccggt gccgcaaggc gagccgggcg aaatctgtgt
     8521 cagcggacca cttttggcgg gcggttactg gaacttaccc gatgagacgt cgcgaacttt
     8581 taaagacggc tggctgcata ccggcgacat ggcccgcgag gacgaggacg gcttctacta
     8641 catcgtcgac agggtcaagg acatgatcgt caccggcggc ttcaacgtgt tcccgcgcga
     8701 agtcgaagac gtggtcgccg agcacgcggc cgtcgcgcag gtgtgtgtgg tcggggcgcc
     8761 cgacgagaag tggggcgagg ccgtcacggc ggtggtggtg ctccgcggcg acgcggcccg
     8821 cgacgacgcc gcgatcgagg cgatgaccgc cgagatccag gccgcggtca aggaacgcaa
     8881 gggctcggtg cagtcaccca agcaggtcgt ggtcgtcgac gcgctgcctt tgaccgggct
     8941 gggcaagccg gacaaaaagg ccgtccgggc gcggttctgg gagggcgccg gccgcgccgt
     9001 cggctgattt cccgcccaga gtgcagccag ccgcacactc gcggcccaac gtgcagctgg
     9061 ccgcacgctc ggcgcgatca ggcgcgatca agaagtcagc gcgacacgcc ggccaccaac
     9121 aggtcgagca tgcgacccgt ctgctccggt gaaccgctgg tcaagaagat gccgatcagg
     9181 ctggacacca catcgtccgc ctgcacctcg ctgcgcaaag tcccgtcgct gacaccggcc
     9241 cccaagagca tgtcgacggc accgacgatg ctctcacgag tctggctggg cgcaagagcg
     9301 ccggagtcga acatggcgtg cagcgattcg gccattccgc gcttggcggc cacgaagctt
     9361 gcgtagcggt ccatccagcg ccgcagcgcc accttcggcg aatgccgctc gagcaactgc
     9421 ccggccgccg cggccacttc ggcgagctcg gtgcgataga tcgcctcgac gagggcttcc
     9481 cgattgggga aatggcgata cagcgtgccg atcccgacgc cggcgtctcg cgcgatcgac
     9541 tccagcgaca ccggccgccc gtcggcggcg gcgaacgccg cggtcgcgac ctcgagcaac
     9601 ttctcgcggt tccggcgcgc gtcggaccga attcgctcgg ccaaagcgga gggtcctccg
     9661 tttctgttac gctgacacaa agcggaggat cctccgcatt ttctccagtg tctcacgagg
     9721 gagcgccatg acagacaagc cagagcccgg cggcgtcggc agcatcggca ccaacgcggt
     9781 tgcccgcatc ggctacggcg ccatgcaact gttcgagaca tcgcccgacg ccgccgccgc
     9841 ggtgctgcgc cgcgctgtcg aactcggcgt caaccacatc gacacggcgt cgttttacgg
     9901 ccccggcgag gtgaaccgcc ggatccgcgc ggcgctaacg ccctacccga acgacctcgt
     9961 catcgtcagc aaggtcggtg cccggtatac cggcgaagaa ccggtacccc tggccgcggc
    10021 ccagcggccc gccgaactga gggccgcagt tgaggatgac ctgagccagc tcggcgtcga
    10081 atgcataccg gtggtgaatc tgcgacgcct ggacgtcggg cccggcctgc aggccgaggg
    10141 cgaccaaatc gtcgacctcg acgaccagct ggccgagatg atcgcgctgc gcgacgaggg
    10201 caagatcggc gggatcggcg tcagcagtgt gccgctcgac gtgctgcggc gggcgctgcc
    10261 ggcggggatc gcctgcgtgc agaacgccta cagcctgctc gaccgctccc aggaggagat
    10321 gctcgacgtc tgcacggccg aaggaatcgc ctgggtgccg tatttcccgc tcggatcggc
    10381 gttcccgggg ttcccgaagg tcgccgacaa acccgtggtg gtggacatcg ccggcgaaat
    10441 cggcgcgaca tcgtcccagg tcgggttggc ctggctgctg gcccacgcgc cgaccacgtt
    10501 gctgattccg ggaacccggt cgatcgtcca ccttgaggag aacatcggcg cggggaacgt
    10561 cacgctgagc gccggggcga tcgcccggct ggacgccgtc accacaccgg gggccgaccc
    10621 gtgggcgcac ggtgtcgaac ccttcttgga ggcgccccga acatgacggg ccctgactac
    10681 tgtggcaggc atggcaaacg ctgatgggct caagccaccg ctcagggcgc cctggtggct
    10741 gaaaccgacg aacaaggtgt tcatccaatt gtcgcgactg ggcttgagtt tcggcggcga
    10801 gagcccagtc gtgttgacgg tgaagggacg caagtccgga cgtgaccggt ccacaccggt
    10861 gacgccgatg accgtcgacg gcaaacagta cgtcgtcgcc ggatttcccg gcgccgactg
    10921 ggtgtccaat gtgcgcgccg ccaacgaggt gacggtggcg cgcggccgcc gcgtccagca
    10981 gctgcgaatg gtggagctgt ccgccgaaga ttcccggccg tttttgcgtg cattcccgac
    11041 gcaggtgccc acgggcgtcg gcttcatgaa gcggtccggg ctgatcaaag agggccgacc
    11101 cgacgagttc gaggcgctgg ccggggtctg tccggtgttc cgattggacc cggtgcagga
    11161 cggagttcaa aagccttgag tgacaaccca tttgatgcgc aggcatggcg acccgtcgac
    11221 ggattcggcg acctgaccga catcacctac caccgccacg tccacgacgc gacggtgcgg
    11281 gtggcgttca accggcccga ggtgcgcaac gcgtttcggc cgcacaccgt cgacgagctg
    11341 tatcgcacgc tcgaccacgc ccggatgtcc cccgacgtgg gcgtggtgct gctgaccggc
    11401 aacggcccct cacccaaaga cggcggctgg gcgttctgct ccggcggtga ccagcggatt
    11461 cgaggtcggt ccggttacca atatgcctcc ggtgagaccg ccgacacggt ggatgccgca
    11521 cgcgcgggcc ggctgcacat cctcgaggtt cagcggctga tccggttcat gcccaaggtg
    11581 gtcatctgcc tggtcaacgg gtgggccgcc ggtggtgggc acagcctgca cgtgatctgc
    11641 gacctcaccc tggccagccg cgagcacgcc cgtttcaagc agaccgatgc cgacgtcggc
    11701 agcttcgacg gcggctacgg cagcgcctat ttggcccgtc aggtgggcca gaagttcgcg
    11761 cgcgagatct tcttcctggg ccggtcgtac accgcggagc agatgcacca catgggcgcg
    11821 gtcaacgagg tcgtcgacca cgccgacctg gagcgggtcg gggtgcagtg ggcggccgag
    11881 atcaacgcga aatcccctca ggcgcaacgg atgctgaagt tcgcgttcaa cctgctcgac
    11941 gacgggctgg tgggtcagca gctgttcgcc ggcgaagcga cccgactggc gtacatgacc
    12001 gacgaggccg tcgagggccg cgacgcgttc ctggaaaagc gcgacccgga ctggagcccc
    12061 ttcccgcgct acttctgagg tcgcgcgcat cgaaatcgac gccaccgcgg gaaagggcga
    12121 gaggaagccc gcgccaatgt cgatctcgac gacatgccgg cgccccctag actcccccct
    12181 gtgagcaaga gtccgtttcg tcggttcacc gagcaaatcg cgctggccgg catgcggccg
    12241 cccagttcgc cgcaggtgct ggtcaaccga cccgcgatca agcccgtcga cctcgacggc
    12301 aagcgagttc tgctcaccgg cgcatcgtcg ggcatcggcg aggcggcggc cgaacagttc
    12361 gcccgccgcg gcgccaccgt ggtcgtggtc gcccgccgcc aggacctgct ggacgcgctg
    12421 gcagcacgaa tcacggcggc gggcggcacc gcagagtcga taccctgcga cgtctcggac
    12481 atggacgccg tcgacgcgct ggtcgccgac gtggaaaagc gcatcggtgg agtcgacatc
    12541 ctgatcaaca acgccggccg gtccattcgc cgtccgctgg ccgagtcgct ggaacgctgg
    12601 catgacgtcg agcggaccat ggtgctcaac tactacgcgc cactgcggct gatccgcggg
    12661 ttcgcccccg gcatgctcga gcgcggcgac ggtcacatca tcaacgtctc gacgtggggt
    12721 gtgctgacgg aagcgtcgcc cctgttcgcg gtctacaacg catcgaaggc cgcgctatcg
    12781 gcggtgagcc gggtggtgga aaccgagtgg ggccgcaagg gggtgcattc gacgacgctg
    12841 tactacccgc tggtggccac gccgatgatc gcgcccacga aagcgttcga ggggatgccg
    12901 gcgctgacgt ccgaagaggc cgccgagtgg atgatcaccg cggcccgcac ccggccggtg
    12961 cggatcgcac cgcgcatggc gattgcggcc aaggcgctgg acagcttcgg accccgctgg
    13021 gttaactcgc tgatggcgcg gcagagcgtc cagcccaacc acgaagcctc gggctaagca
    13081 gtgacgacgc cgcagagcgc tcgaccttca ggagtttgct gtgtctgacg tctggaccac
    13141 tatctccgcc gagcgcggtg cgcttgccgg cgacctcgcc gacctgaccc cggcgcagtg
    13201 ggacacgccg tccctctgcg ctggatggac ggtccgtgac gtcgtcgcgc acctgtcggc
    13261 gacggcctcg ctgaaccccg ccctgttctt cctcgggatg gccagggcgc ggttcaactt
    13321 cgacaggttc gccaacgaac ggatcgccag gcaccgcggc gccgacccgg tcgccaccct
    13381 ggcggagttc cgcggcctgc agaactccac ctcggcgccg ccgggtccca ccacgtcctg
    13441 gctgggcgag gtggtggtgc acggcgccga cattcgtcgc ccgctgggca tcccgcacac
    13501 ctatgcccgc ggtgcggttc gccaggtgat cgacttctac aagggctcca acctgctcat
    13561 cggctccaag aaccgcatcg cggggctggc gctgcatgcc accgaccacg actggcggca
    13621 cgggcagggg gagcgcgacg cctgggtcga gggcccattg ctgtcgctac tgctcgccat
    13681 gaccggccgg gccgccgcct gcgacgatct catcggccct ggcgtggcaa cgctgcgcag
    13741 ccgatgcgcg ccgaaccgat agcgacgtga tagacacaac gctatggaga tcctggccag
    13801 ccggatgctg ctccggccgg cggactatga gcggtcgttg agcttctacc gcgacgagat
    13861 cggcctggca atagcccgcg attacggggc cggcacggta tttttcgcgg gtcagtcttt
    13921 gctggagctg gccggttacg gctcccccga ccactcccgc ggaccctttc ccggtgcgct
    13981 gtggctgcag gtccgcgaca tcgcggcaac ccaggccgag ctgcaaggcc ggggggtgcc
    14041 gatcgcccgc gaggcacgcc gcgaaccctg gggcctgcac gagatgcacg tgaccgaccc
    14101 agacggaatc acgctgattt tcgtcgaggt gcctcccggt cacccgctac gccgcgacgc
    14161 ccggcaagac cgtggcgcaa cgcccggtta actgggcgct aacatctgac gacaagtcaa
    14221 ggtacacccg cgatttggct ctctgtgcgt tcgacaattt aatcccccta ctaccagaat
    14281 caggcgctgt gaccatcgac ttgttcctcc taatcatcgt cgtaatcacc gcactcggct
    14341 tcgacttcac caacggattc cacgacaccg gcaacgcgat ggcaacctcc atcgccagcg
    14401 gtgcgctgaa acccaaggca gccgtcgccc tgtccgcggt cctcaacctc gtgggcgcct
    14461 tcatgtccac cgccgtcgcg gccacgatcg ccaaagggct gatcgacggg cacatcgtga
    14521 cgctggaact tgtctttgcc ggcttggtcg gcggtatcgt ctggaacctg ctgacctggc
    14581 tcttcgggat cccctcgagc tcctcgcacg cactgatcgg cggcatcgtc ggcgcgacga
    14641 tcgctgccgt cggcgcgcac ggggtgctct ggaagggcct ggtgtccaag gtgctggtcc
    14701 cggccgtcat cgccgcgatc ctggccatca tggtcggggc gctggccacc tggctggtgt
    14761 accggatcac ccgcggcgtc ccggagcagc gcaccgaggc cgggttccgg tacggccagt
    14821 ggggctcggc gtcgctggtc tccctggcgc acggcaccgg cgacgcgcag aagacgatgg
    14881 gcgtcatctt cctggcgctg atgtcctacg gggcgatcag caagagcgcc gcggcgcctc
    14941 cgctgtgggt catcggggct tgcgcgctgg ccatggcgat gggtacctac ctgggtggct
    15001 ggcggatcat ccgcaccctg ggcaagggac tggtcgagat caaatcgccg cagggcatgg
    15061 ccgccgaatc gtcctcagcc gcggtcattc tgctgtccac ccacttcggc tacgcgctgt
    15121 cgacaacgca ggtctgcacc ggttcggtgc tgggcagcgg gctgggcaag cccggtggcg
    15181 aggtgcgctg gagcgtggcc ggccggatgg cggtcgcctg ggtgttcacg cttccgttgg
    15241 ccggtttggt cggggcgatc acctactgga tcgtgcacga aatcggtgga taccccggcg
    15301 cgatcatcgg cttcgggatg ctggtcgcgg tcgcttcggc catctacatc cggtcgcgga
    15361 aggtcaaggt cgaccacaac aacgtcaacg cggagtggga aggcagcctg accgccggtc
    15421 tcgacgcttc ggacgaccat cagccgccgt ccgataccgg gcccaaaata ggcaccgggt
    15481 cgccccggtc gggctctaac gactccacgg tgatcgcggg gaacgcatca tgagccacct
    15541 tagcgaatgg ttcaattacc aggcctcctt gaagattttt atcttcgcat tgctggccgg
    15601 tgccgcactg ccggggcttt tcgccctcgg gctccggctg cacgtcgtgg gcggcggaca
    15661 agtcagcgtt ggcggcgcga caccacacaa gaatccggcg ctcctggcgc tctcctacgc
    15721 gatcttcgcg ctggtagtcg cggtgatcgt gttcgccctg ctgtatgtct cccgcgactt
    15781 cctcgcgcac acgacgggtc acccgtttct aggcgccaag cccaagtagc cccgcgtagc
    15841 attgaatcgt tgtccacata actgccgatc ccctcgcgtg gagagatagc caagtgaacc
    15901 gattcctcaa ttcggtcgtc tcgtggttgc gcgcgggata cccggacggc atcccaccga
    15961 ccgacacgtt cgcggtgctc gccctgctga cccgccggct gagcaacgac gaggtcaaag
    16021 cagtggctca tgagttcatg cagcgcggcg atttcgatga cgtggatatc ggcgtcgcga
    16081 tcagcaagct gaccgacgag ctgccgtcgc ccgaagacat cgagcgggtg cagacacggt
    16141 tggccgccaa gggctggccg ctcgacgagg ggcgcgacgc cggagacccc gtatagccct
    16201 cctgcgcgca ctcagcgtcg catccggctc tgccgcagcg tcgctgatgc ccgcgctgga
    16261 gcgcgtgctg gtcggccggg actccgcgct ggtcgcggtc ccggccgacg gagatcacga
    16321 ccccgagctg gacgctttgc gggcggggtt gcgggtgggc gaggagatcg acgacgacgt
    16381 ggccctcgtg gcggtcacgt cgggaatcac cagcacaccc aagggcgccc tgctgacccc
    16441 cgccgccttg acggccagcg cgtcggccac ccacgagcgt ctcggcggac ccggcagctg
    16501 gctgctggca ttgccgccgc atcacatcgc cggcgtccag gtgctggtgc gcagcgtgct
    16561 ggccggcacc gtgcccgtag agctggacgt ctccgcaggg ttcaacgtcg ccgagttgcc
    16621 cggcgccgtg cggcggttgg gctccggccg tcgctacgcg tcactcgtcg ccacccagct
    16681 ggccaaggcg ctcgccgacc cggccgcggc gtacgcgctg gccgaactgg atgcggtgct
    16741 gctcggcggc ggaccggcgc cgcggccgat cttggacgcg gcggcggcgg cgggcatcac
    16801 ggtggttcgc acctacggga tgagcgagac ggcgggcggc tgcgtctacg acggcgtccc
    16861 gctggacggg gtgctgctgc gggtgccggc cgacgggcgc atccagatcg gcggcgcgac
    16921 cctggcgaag gggtaccgca accccgtgga tcccgacccg ttcgccgagc cgggctggtt
    16981 cagcaccgac gacctgggca ccgtcaatga ggcgggcgtg ctgaccgtgc tgggccgcgt
    17041 cgacgacgcg atcagcacgg gcgggttgac cgtgctcccg cagccggtcg aggccgcgct
    17101 gtgcactcac cccgccgtta gtgagtgcgc ggtcttcggc cttgccgacg accggctggg
    17161 ccagcgggtg gttgccgcga tcgtggtggg cgacgggtgc gaagcgccga cgttggacgc
    17221 gctgcgggcc caggtgaccc gcacgttgga tgccaccgcc gcaccgcgcg agctgcacct
    17281 cgtcgccgcg ctgccgcgac gcgggatcgg caaggtcgac cggacggcac tggtgcgccg
    17341 gttctcccgc gccgacgatc aataggctgg tcgatcgtga aggtcgcgcg gcgggaatca
    17401 ctggccgtcg cggtcggcgt cgtcctggtg gcggcggcgg tgctgctgcc gtggctggac
    17461 cggggcatcg tgccgcgact cgacatcggg ccacagccgt tcgccaccca cgcccaagcc
    17521 gcaccgatct tcggcacgtg ggagattcac gcgagctggg ggaccgggcc ggccattctc
    17581 atcgccctgg ccgccgtggc gtggggaccc ggcgtcgcac aacgtctttc gtggcggacg
    17641 ctgacgctgg gcacgtgggc aaccgcctgc gcgtgggcgt ttgcgctcgc aatgatcgac
    17701 ggctggcaac ggggcttcgc cggccgattg accaccaggg acgagtacct gtcgcaggtg
    17761 ccgacgatca ccgacattcc cgccgcgctc cgcacgttca ccagccggat cgtcgactac
    17821 cagccgaact cctgggtcac gcacgtctcc gggcatccgc ccggcgcgct tctgacgttc
    17881 gtctggctgg accggatcgg cctgcacggc ggcgcctggg ccgggctgct gtgcctgctg
    17941 gccggctcca gcgccgcggc ggccgtactg atcggtgtcc gtgcgctggc cgacgagcag
    18001 accgcgcggc gcgccgcgcc gttcatcgcg gtggcgccga cggcgatctg gatcgcggtc
    18061 tcggccgacg ggtacttcgc cggagtcgcg gcgtggggca ttgcgctgtt ggcggtggcg
    18121 gtgcaccgcc ccgttcggtt ccccgcgctg gtcgccgcgg cggccggtct gctgctgggc
    18181 tgggcggttt t
//
LOCUS       DASSGQ010000093         4904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_735617,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000093 DASSGQ010000000
VERSION     DASSGQ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735617"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..1377
                     /gene="pstS"
                     /locus_tag="VFI55_04200"
     CDS             265..1377
                     /gene="pstS"
                     /locus_tag="VFI55_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET7073271.1"
                     /translation="MKRNRFGAALSILTTAAVLLSACGADNNVTGGSATTGTSSATVA
                     CGGKPTLTASGSTAQQNAMTRFVKAFEQACQGQSLNYTPNGSGAGISEFEGNQTDFGG
                     SDSPLSKDDYGRAQQRCGSPAWNLPVVFGPIAIAYNVNGVTSLSLDGPTAAGIFNGDI
                     TTWNDSQIQALNLGVTMPAEPIRVVFRSDESGTTDNFQKYLDSASNGIWGKGAGKKFN
                     GGVGEGAKGNDGAAGAVKATEGSISYVEWSFAQAQHLNMAGVVTSAGPEPVAISAASV
                     GKTISAAWFIKKGNDLALDTISFYRPNRPDSYPIVLATYEIVCSKYPDSQVGAAVKAF
                     LQIAIRAGQDGLADNGYVPIPQEFKTRLSTAVDAIS"
     gene            complement(1406..1618)
                     /locus_tag="VFI55_04205"
     CDS             complement(1406..1618)
                     /locus_tag="VFI55_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073272.1"
                     /translation="MFTKVLLSVIVGLVAGVVVAAPAGADPSVFSKLSCSCTDGSTVS
                     NYAPATIDRINEGIQSGMSALPDVPQ"
     gene            complement(1701..2042)
                     /locus_tag="VFI55_04210"
     CDS             complement(1701..2042)
                     /locus_tag="VFI55_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HET7073273.1"
                     /translation="MTTRRARLPRPVRSLSAGLVLLVALLGLPEVAIPAAHADAIDNS
                     FLSAIKAKGITFASPEDAIVAGHEVCDELDLGRQKSDVASEVMNNAKMDDYHAGFFVG
                     VSVAAYCPRYR"
     gene            2224..3141
                     /locus_tag="VFI55_04215"
     CDS             2224..3141
                     /locus_tag="VFI55_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HET7073274.1"
                     /translation="MSYVRWFSLAVLAVCVAGCGMRDVSAASARELPGTFRSGGMDRT
                     YTLHVPPGDPVGLVLSLHGGGGSGSGQRDLTDFDAVADANNLLVVYPDGYDKSWADGR
                     GASPADRHHVDDVGFLVGLVSKLQSDYGIARGHVFATGMSNGGFMSNRLACDRADVFA
                     AIAPVAGTLGAGVACNPSRPVSVLQAHGTADPLVPFNGGDVRGRGGVSHSISANGIVN
                     KWRGANGCEGDPSVQVLPNVGDGTVVHRFDSVACAASTEVVFYQIDNGGHTWPGGKQY
                     LPQAIIGRTSRALDASEVIAQFFLVHARN"
     gene            complement(3148..3903)
                     /gene="nei2"
                     /locus_tag="VFI55_04220"
     CDS             complement(3148..3903)
                     /gene="nei2"
                     /locus_tag="VFI55_04220"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII Nei2"
                     /protein_id="HET7073275.1"
                     /translation="MPEVDTVWHTAATLREHLAGQTLTRCDIRVPRFATVDLTGQVVD
                     EVLSRGKHLFIRVGPVSIHSHLKMDGTWRVGGRTVRVDHRTRIVLESNDIRAVGTDLG
                     VLEVLERDRDGDAVAHLGPDLLGPDWDPARAAANLTAGPDRPIGEALLDQRVLAGVGN
                     VYRNELCFVSGHLPTAPVAAATDPLRLVSRARDMLWANRFRWNRCTTGDTRAGRQLWV
                     YGRAGLPCRRCGTRINYDDAGERVTYWCPSCQR"
     gene            complement(3917..>4904)
                     /locus_tag="VFI55_04225"
     CDS             complement(3917..>4904)
                     /locus_tag="VFI55_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217813.1"
                     /note="similar to the DNA and RNA helicase superfamily II
                     and eukaryotic DEAD family of helicases; longest known
                     protein in E. coli; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HET7073276.1"
                     /translation="APAEIDFTDAHRAILDTLAGGGAYFFRQLAHQEIGEAALKAALW
                     ELIWAGWITGDTFAPVRALLSGVGARKRSAPAHRSHRPPRLTRYSVAHPQLRSADPTV
                     AGRWSALPAPEPDSTVRAHYQAELLLNRHGVLTRGAVAAEGLPGGFAMLYKVLGTFEE
                     AGRCQRGYFVESLGGAQFAVASTVDRLRSYLDGIDPERPEYRAVVLAAADPANPYGAA
                     LPWPTSSGESPGARPGRKAGALVVLVDGELAWFLERGGRSLLTFTDDAAADHAAAAAL
                     ADLVAARRVASILVERIDGTPALEPRAGGPGPVGAALSDAGFVRTPRGMRLR"
BASE COUNT          822 a         1603 c         1662 g          817 t
ORIGIN      
        1 tgacttgccc gctcacgggg atccgcgcct gcggtggcta actccgcggc ctgctgcgcg
       61 gacccaggga tcctcagggc ccggaaaagt tcgagcaacc gtggccgaga ttctgaatcc
      121 ggcgatgtcc agccaaagcc acacatcacc gacccaacgg cgatcttgtt attatgcctc
      181 agggctgcga ccaagacggc agtctgacgg ggtgcggcta cgccggcggc gaccatctca
      241 tctgtaccat tcaaaggaat tgaattgaaa cgcaatcgat ttggcgccgc gctgagtatt
      301 ctcaccactg ccgcggtgtt gttgtcggcc tgtggtgctg acaacaacgt aaccggggga
      361 agtgcgacga ccggcacgtc gtcggcgacg gttgcatgcg gtggaaagcc gactttgacg
      421 gcgagcgggt cgacggccca gcaaaatgca atgaccaggt ttgtcaaggc tttcgagcaa
      481 gcctgccagg ggcagtccct gaattacact ccgaacggtt ccggcgccgg aattagcgaa
      541 ttcgagggca accaaaccga tttcggtggc tcggactcgc ccctgagcaa ggacgattac
      601 ggcagagcgc agcaacgctg tggctcgccg gcgtggaact tgccggtcgt gttcggcccc
      661 atcgccattg cctacaacgt caacggtgtg acctcgttga gcctcgacgg tccaaccgca
      721 gccgggatct tcaacggcga catcaccacc tggaacgatt cccagatcca ggcgctgaac
      781 ttgggcgtca caatgccggc tgagccgatt cgcgtggtgt tccgtagcga tgagtccggc
      841 accacggaca acttccagaa atatctcgac agcgcttcca acggcatatg gggcaagggt
      901 gccggaaaga agttcaacgg tggtgtcggc gagggcgcca agggcaacga cggcgccgcc
      961 ggggccgtca aagccaccga agggtcgatc agctacgtcg aatggtcatt cgcccaggcg
     1021 cagcacctga acatggccgg tgtcgtcacg tcggccggtc cggagccggt ggcgatcagc
     1081 gcagcctcgg tgggcaagac gatctctgcc gcctggttta tcaagaaagg caacgacctg
     1141 gccctcgaca cgatctcctt ctatcggcca aacaggcccg actcctaccc gatcgtgttg
     1201 gcgacctatg agatcgtgtg ctccaagtac cccgattcac aggtcggtgc ggccgtgaaa
     1261 gcgttcctgc aaatcgcgat ccgcgccggc caggatggtt tggcggacaa cggctatgtc
     1321 ccgattccac aggagttcaa gacgcgattg tcgacggcag tcgacgccat ctcgtgaggt
     1381 gaggcggctt tcggcctccc gcaggttatt gcggcacgtc tggcagagcc gacatgccgc
     1441 tctggatccc ctcgttgatt cggtcgatgg tggcaggcgc gtaattcgag accgtggatc
     1501 cgtcggtaca gctacagctg agcttgctga acacgctggg gtcagcgccg gccggcgctg
     1561 ccaccaccac ccccgcaacg agcccgacga tgacactcaa caacactttc gtgaacattg
     1621 ccggctccct gtgatatcag tcactttgag tctaggcgtc cccgtctcga gtggtccacg
     1681 ggcgaaatgc gttaggtcga tcagcgatac ctggggcagt acgcggccac gctgacgccg
     1741 acaaagaacc cggcgtgata gtcgtccatt ttggcgttgt tcatgacctc ggacgcgaca
     1801 tcggacttct gcctgccgag atcgagttcg tcgcagacct cgtgaccggc gacgatcgcg
     1861 tcttctggtg acgcgaaggt gatgcccttc gctttaatcg ccgacaggaa cgagttgtcg
     1921 atggcgtcgg cgtgcgcggc ggggatcgcc acttcgggga ggccgagcag cgcgaccagc
     1981 aggactaagc cggccgatag cgatcgcacg ggtctgggca agcgcgcgcg gcgagtcgtc
     2041 attgctggaa cctccccgct gcgctgtgat gacggcaagc cgtgccgatg atagtgcgat
     2101 catggcatgc cgcgaccgga cgggtgaaag tccctcggtc cgcccgagac ccgcgcggtg
     2161 tcctcgacac caagacctgg cccgctttcg tcgatcggcg cgcgggatcg gtaatttggc
     2221 ggcatgtcgt acgttcgctg gttttcgctc gccgttctgg cggtctgcgt ggccggctgc
     2281 ggcatgcggg atgtgtcggc ggccagcgct cgagagctgc ccgggacgtt tcgttccggc
     2341 ggcatggacc ggacttacac gctgcacgtg ccgcccggcg atcccgtcgg cttggtcctc
     2401 agcctgcacg gtggtggggg tagcggaagc gggcagcgtg atctgaccga cttcgacgcc
     2461 gtcgccgacg ccaacaacct gctggtggtc tatcccgacg gttacgacaa gagctgggcc
     2521 gacgggcggg gtgcgtctcc ggcggatcgc caccatgtcg acgatgtggg atttttggtc
     2581 ggattggtga gcaagctgca aagcgattac ggcatcgccc gcggacatgt ctttgccact
     2641 ggcatgtcca acgggggctt catgtccaac cggctcgcgt gtgaccgcgc cgacgtcttc
     2701 gcggcgatcg cgccggtggc ggggacgctg ggcgccggtg tggcctgcaa tccgtcgcgg
     2761 ccggtgtccg tcctgcaggc gcacggaacc gccgatccgc tggtcccgtt caacggcggg
     2821 gacgtgcgcg gacgcggcgg agtcagccac tccatctcag ccaacggcat cgtgaacaag
     2881 tggcgcgggg cgaacgggtg tgagggcgat ccgtcggtgc aggtgctgcc gaacgtcggg
     2941 gacggcaccg tggtccaccg cttcgactcc gtggcgtgtg cggcgtctac cgaagtcgtc
     3001 ttctaccaga tcgacaacgg cggccatacc tggccgggcg gcaagcagta tttgccccag
     3061 gcgatcatcg ggcgcacttc gcgcgcgctg gacgcctcgg aagtcatcgc acagtttttt
     3121 ctggtgcacg cccgcaacta gccgggttca gcgctggcag gacgggcacc agtacgtcac
     3181 ccgctcgccg gcgtcgtcgt agttgatgcg ggtgccgcag cggcggcagg gcagccccgc
     3241 tcgcccgtac acccacagct gccggcctgc ccgggtgtcg ccggtggtgc agcggttcca
     3301 gcggaaacgg ttggcccaca gcatgtcccg ggcgcgagag accagccgaa gggggtcggt
     3361 ggccgcggcg accggcgcgg tgggcagatg cccgctgacg aaacacagtt cgttgcggta
     3421 gacattgccg accccggcca gcacccgctg gtccaacagc gcctcgccga tcggccgatc
     3481 cgggcccgcc gtcaggttgg cggcggcccg cgcgggatcc cagtctggtc cgaggagatc
     3541 gggtcccagg tgcgcgaccg catcgccgtc ccggtcgcgc tccaagactt ccagcacgcc
     3601 caggtcggtg ccaaccgcac gaatatcgtt tgattccaat acgattcgtg ttcgatgatc
     3661 cacccgtacc gtgcggccgc cgacgcgcca ggtgccgtcc atcttcaggt gcgagtgaat
     3721 gctgaccggc ccgacccgga tgaacaagtg ctttccccgg ctgagcactt cgtcgaccac
     3781 ctgcccggtg aggtcgacgg tggcaaagcg cggcacccgg atatcgcagc gcgtcagagt
     3841 ctgccccgcc aggtgctccc gcagcgtggc cgccgtgtgc cagacggtgt caacctcggg
     3901 cattagtcac cacaggtcac cgcagccgca ttccgcgcgg ggtgcggacg aagccggcgt
     3961 ctgagagcgc cgcgccgacg gggccgggtc cgccggcacg tggctccaat gcaggcgtcc
     4021 cgtcgatccg ttcgacgaga atcgatgcga cgcgccgcgc cgcgaccaaa tcggccagtg
     4081 ccgcggcagc ggcatggtcg gccgcggcat cgtcggtgaa tgtcagcagc gaccggccgc
     4141 cgcgctcgag gaaccaggcc agctcgccgt ccaccaacac gacgagcgcc cccgccttgc
     4201 ggccgggccg cgccccgggg ctttcaccgc tggatgtcgg ccagggcagt gcggcgccgt
     4261 aggggttggc cgggtcggcg gctgccagca cgacggcccg gtattccggc cgttccggat
     4321 cgatcccgtc gaggtagctg cgcagccggt ccacggtcga ggcgacggcg aactgggcgc
     4381 cacccagcga ctcgacgaaa tagccgcgct ggcaccggcc ggcctcctcg aacgtgccca
     4441 gcaccttgta gagcatcgcg aacccgcccg gcaggccctc ggccgccacg gcgccccggg
     4501 tcaacacacc gtggcggttc agcagcaact cggcctgata gtgcgcccga acggtggaat
     4561 ccggctcggg agccggcagg gcggaccatc gcccggccac ggtcgggtcg gcgctgcgaa
     4621 gctgagggtg cgcgacgctg tagcgggtca gccgcggcgg gcgatgggac cggtgcgctg
     4681 gggcggaacg cttgcgcgcg ccgacgccgc tgagcagggc acgcaccggt gcgaacgtgt
     4741 cgccggtgat ccagccggcc caaatcagtt cccacagtgc ggccttgagc gccgcctcgc
     4801 cgatttcctg gtgggcgagc tggcggaaga agtacgcgcc acctccggcc agtgtgtcca
     4861 ggatcgcgcg gtgggcgtcg gtgaagtcga tctccgcggg tgcg
//
LOCUS       DASSGQ010000094        21523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_310398,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000094 DASSGQ010000000
VERSION     DASSGQ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 21523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21523
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310398"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..290
                     /locus_tag="VFI55_04230"
     CDS             75..290
                     /locus_tag="VFI55_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073277.1"
                     /translation="MSETLIWMKHGDDYRATGDEDREYFVHPLPGSAEFPWEAGTTAP
                     REPCKERSRVAAQRLCQAWEDGLALTD"
     gene            369..881
                     /locus_tag="VFI55_04235"
     CDS             369..881
                     /locus_tag="VFI55_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073278.1"
                     /translation="MDDDTASTDVTVWYRADLFGIPPGEEASYSQDWYVYAVSDLKAA
                     RAFAALREDQEGTPRSVYRVRLIGEVETDPDYRSFPEFVRCLSGIVQEVIEAHPTMSP
                     DEATEYMCQTYNPPWPGGGGPMYDGDGYPTASPAMLGAGISANELRHVGKCKPPLIIG
                     GYAEGLVNRA"
     gene            complement(1048..1124)
                     /locus_tag="VFI55_04240"
     tRNA            complement(1048..1124)
                     /locus_tag="VFI55_04240"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1088..1090),aa:Pro,seq:cgg)
     gene            complement(1142..2290)
                     /locus_tag="VFI55_04245"
     CDS             complement(1142..2290)
                     /locus_tag="VFI55_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HET7073279.1"
                     /translation="MNDLTQIVTRSLPRSWTDNAIRLIQADARRSADTHLLRYPLPSA
                     WCAEVDVALYLKDETTHITGSLKHRLARSLFLYALCNGWIGEGTTVVEASSGSTAVSE
                     AYFAALLGLPFVAVMPAATSAAKVALIESQGGRCHFVPDSSRVYAEAERVARETGGHY
                     LDQFTNAERATDWRGNNNIAESIYEQMRDEKYPVPEWIVVGAGTGGTSATIGRYIRYR
                     RHATRLCVVDPENSAFFPAYAEERYDIVMPTSSRIEGIGRPRVEPSFLPGVVDRMVAV
                     PDAASIAAARHVSAVLGRRVGPSTGTNLWGAFGLLAEMVAGGRGGSVVTLLADSGDRY
                     ADTYFSDEWVSAQGLDPSGPAATLVEFERDCRWSGLQPAVSPADGPVR"
     gene            complement(2287..3246)
                     /locus_tag="VFI55_04250"
     CDS             complement(2287..3246)
                     /locus_tag="VFI55_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7073280.1"
                     /translation="MPDVLPALIRTGAVALGSTVAGIGYAALVERNAFVLREITMPVL
                     TPGSTPLRVLHISDLHMLPNQRRKQAWLRELVSWEPDLVVNTGDNLAHPKAVPAVVQT
                     LGDLLSRPGVFVFGSNDYFGPRLKNPLNYLTNPSHRVRGEPLPWQDLRAAFTERGWLD
                     LTHTRREFEVAGLQIAAAGVDDPHIDRDRYETIAGPASAAANLRLGLTHSPEPRVLDR
                     FAADGYQLVMAGHTHGGQLCLPFYGALVTNCGIDRSRAKGPSRWGANMRLYVSAGIGT
                     SPFAPVRFCCRPEATLLTLIATPMGDRDSSRDLSRSQPTASVR"
     gene            complement(3303..5732)
                     /gene="ponA2"
                     /locus_tag="VFI55_04255"
     CDS             complement(3303..5732)
                     /gene="ponA2"
                     /locus_tag="VFI55_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_178005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase/D,D-transpeptidase PonA2"
                     /protein_id="HET7073281.1"
                     /translation="MSDRPPAALTLIKLAGCCLLASVVATALMFPLVGGVGLMSNRAS
                     EVVANGSAQLLQGEVPAVSTMVDKKGNVIAWLYSQRRFEVPTDKIANTMKLAIVSIED
                     KRFADHNGVDWKGTLTGLAGYASGDVGTRGGSTIEQQYVKNYQLLVTAQTDAEKRAAV
                     ETTPARKLREIRMALTLDKTFSKPEILTRYLNLVSFGNNSFGVQDAAQTYFGINASDL
                     NWQQAALLAGMVQSTSTLNPYTNPDGALARRNLVLDTMIDNLPQETDALRAAKAEPLG
                     ILPQPNELPRGCIAAGDRAFFCDYVQEYLSRAGINKEQVARGGYTIRTTLDPDVQAPV
                     KSAIDKYASPTLPGISSVMSVIAPGKDSHHVMAMASNRKYGLDLDAGETMRPQPFSLV
                     GDGAGSVFKIFTTAAALDMGMGINATLEVPGRFQAKGMGSGGAKGCPKDTWCVINAGN
                     YRGSMNVTDALATSPNTAFAKLIQQVGVTRTVDMAIKLGLRSYADPGTARDYNPDSNE
                     SLADFVKRQNIGSFTLGPIEVNALELSNVAATLASGGVWCPPSPIDKLIDRNGNEVAV
                     TTETCDQVVPEGLANTLANAMSKDALGAGTAAGSAGAAGWDLPVSGKTGTTEAHRSSG
                     FVGFTNHYAAANYIYDDSTTPTDLCSAPLRHCGEGDLYGGNEPARTWFTAMKPIATNF
                     GPVQLPPTDPRYVDGSPASRVPSVSGLDVDAARQRLKDAGFQVADQTNSVNSSAKFGE
                     VVGTTPNGQTIPGSIITIQVSNGVPPAPPPPPEGGPPPPVGSQVVEIPGLPPITIPLL
                     APPPPPGAPPP"
     gene            6094..6444
                     /locus_tag="VFI55_04260"
     CDS             6094..6444
                     /locus_tag="VFI55_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HET7073282.1"
                     /translation="MSGTRPTARRTNIAAAQGVLHSVETEERIAWVSQALCRTTDPDE
                     LFVRGAAQRKAAVICRHCPVMQECGADALDNKVEFGVWGGMTERQRRALLKQHPEVIS
                     WADFFDKRRNRSIG"
     gene            complement(6813..7970)
                     /locus_tag="VFI55_04265"
     CDS             complement(6813..7970)
                     /locus_tag="VFI55_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HET7073283.1"
                     /translation="MSVTPKVLDMASILADRTNRVVVCCGAGGVGKTTTAAAMALRAA
                     EYGRTVCVLTIDPAKRLAQALGVNDLGNTPQRVPLAPEVTGELHAMMLDMRRTFDEMV
                     VQYSGAGRAQAILDNQFYQTVATSLAGTQEYMAMEKLGQLLSQDRWDLVVVDTPPSRN
                     ALDFLDAPKRLGSFMDSRLWRLLLAPGRGIGRLVTGAMGLAMKAISTVLGSQMLADAA
                     AFVQSLDATFGGFREKADRTYALLKQQGTQFVVVSAAEPDALREATFFVDRLSQEGMP
                     LAGLVLNRTHPTLCSLPVERAIDGSEELDAEKDSDAASLASAVLKIHADRGQTAKREV
                     RLLSRFTGANPHVPVIGVPSLPFDVSDLDTLRALADQITAVGDDAARASGS"
     gene            complement(7967..8989)
                     /locus_tag="VFI55_04270"
     CDS             complement(7967..8989)
                     /locus_tag="VFI55_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HET7073284.1"
                     /translation="MVTSTSSDGNSVGWPARLSKARLHFVTGKGGTGKSTVAAALALT
                     LAAGGRKVLLVEVEGRQGIAQLFDVPPLPYQELKIATAERGGQVNALAIDIEAAFLEY
                     LDMFYNLGIAGRAMRRIGAIEFATTIAPGLRDVLVTGKIKESVVRVDKDKRPVYDAVV
                     VDSPPTGRIARFLDVTKAVSDLAKGGPVYSQSESVVNLLHSEQTAIHLVTLLEALPVQ
                     ETLEAIEELAEMELPIGSVIVNRNIPSYLDPRDLAKAAEGDVDADSIRAGLETAGIKL
                     NDTDFAGLLTETIEHGTRIAARAETAQQLDALKVARLELPAISDGVDLGSLYELSESL
                     AEQGVR"
     gene            9096..9257
                     /locus_tag="VFI55_04275"
     CDS             9096..9257
                     /locus_tag="VFI55_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_178004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HET7073285.1"
                     /translation="MSQPTAWEYATVPLLTHATKQILDQWGADGWELVSVLPGPTGEQ
                     HVAYLKRPK"
     gene            9268..9723
                     /locus_tag="VFI55_04280"
     CDS             9268..9723
                     /locus_tag="VFI55_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET7073286.1"
                     /translation="MSVSARLEQLGVVLPQAVAPLAAYVPALRTGNLVYTAGQLPIEG
                     GKLARTGKVGAEVGPEEAKVLARICALNALAAVDSLVGIDAVTQVVKVVGFVASAPGF
                     NGQPGVVNGASELLAEVFGDKGAHARSAVGVSELPLDAPVEVELIVEVG"
     gene            9729..10520
                     /locus_tag="VFI55_04285"
     CDS             9729..10520
                     /locus_tag="VFI55_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7073287.1"
                     /translation="MSEAVESLTHPAYGRLRAVTDTASVLLADNPGLLTLEGTNTWVL
                     RGPGSDELVIVDPGPDDDEHIARVAAVGRIALVLISHRHSDHTDGIDKLVELTGATVR
                     SAGSGFLRGLGSELIDGEVIDAAGLKITVLATPGHTADSLSFVLDDAVLTADTVLGRG
                     TTVIDKEDGSLAAYLESLRRLRGLGRRTVLPGHGPELADLGAVASGYLTHREERLQQV
                     RAALADLGADATARQIVEHVYVDVDEKLWDVAEWSVQAQLDYLRR"
     gene            complement(10714..11388)
                     /gene="crp"
                     /locus_tag="VFI55_04290"
     CDS             complement(10714..11388)
                     /gene="crp"
                     /locus_tag="VFI55_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HET7073288.1"
                     /translation="MDEVLARAGIFQGVDPNAVAALTKQLTPVDFPRGHTVFAEGEPG
                     DRLYIITAGKVKIGRRSPDGRENLLTIMGPSDMFGELSIFDPGPRTSSATTITEVRAV
                     SMDRDALRAWIADRPEIAEQLLRVLARRLRRTNNNLADLIFTDVPGRVAKQLLQLAQR
                     FGTQEGGAMRVTHDLTQEEIAQLVGASRETVNKALADFAHRGWIRLEGKSVLICDSER
                     LARRAR"
     gene            complement(11485..11880)
                     /locus_tag="VFI55_04295"
     CDS             complement(11485..11880)
                     /locus_tag="VFI55_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073289.1"
                     /translation="MFTLLVSWLLVACVPGLLMLATLGLGRLEKMLAHDTVTASDVDE
                     FLDHAEAVDMHTLALEGMPEALEYLHRRQAGRLADSPALGPNTAPRHGAPLFATAFGD
                     QDGAGLPTRIRTQSHVNPQFRGTRHANRV"
     gene            12054..12734
                     /gene="nth"
                     /locus_tag="VFI55_04300"
     CDS             12054..12734
                     /gene="nth"
                     /locus_tag="VFI55_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET7073290.1"
                     /translation="MNRALAQAFPDAHCELDFTTPLELTVATILSAQSTDKRVNLTTP
                     ALFKRYPSALDYAKADRAELENLIRPTGFFRNKASSLIGLGQALVERFGGEVPSTLAE
                     LVTLPGVGRKTANVILGNAFGVPGITVDTHFGRLVRRWRWTAEEDPVKVEHAVGELIE
                     RSEWTMLSHRVIFHGRRVCHSRKPACGVCVLAKDCPSFGLGPTEPLLAAALVQGPEKE
                     HLLALVGL"
     gene            12751..13407
                     /locus_tag="VFI55_04305"
     CDS             12751..13407
                     /locus_tag="VFI55_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET7073291.1"
                     /translation="MTPTTRWTIAILAVVAALTAALVAELRDDSATTSTVATDQAPAA
                     REHRDADTPAALAGPRQRADLPPCPAAAPGFSGPGAAALQGVTAECAADGSVVDVARA
                     LAGRRVVLNLWAYWCAPCTAELPAMADYQRRVGPDVMVVTVHQDENETAALLRLAELG
                     VRLPTLQDGRRLIPAALRVPNVVPATLVLRPDGSVAQTLLRDFASADEIAAAVGNGTG
                     "
     gene            13429..14244
                     /locus_tag="VFI55_04310"
     CDS             13429..14244
                     /locus_tag="VFI55_04310"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HET7073292.1"
                     /translation="MSAGGTPLRSGDPTCSRGTPPQTPGVCPSWLRPLVDNVDQIPDA
                     YRRRLPPDVLAMLTEARAAAQSSSPGDDREAAVLVLFSGPESGPANGRIPDDADLLLT
                     VRASTLRHHAGQAAFPGGAADPGDAGPVATALREAHEETGIDVSRLHPLATLARAFIA
                     PSQYEVVPVLAYSPDPGPVAVVNETETAIVSRVPVRAFVNPANRLMVYRGDLGRRWSG
                     PAFLLNQMLVWGFTGQVISAVLDVAGWAVPWDTTDVRELDEAMMLVGDEGGLR"
     gene            14250..15443
                     /gene="marP"
                     /locus_tag="VFI55_04315"
     CDS             14250..15443
                     /gene="marP"
                     /locus_tag="VFI55_04315"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance serine protease MarP"
                     /protein_id="HET7073293.1"
                     /translation="MTPSQWLDVAVLAVAFIAAISGWRSGALGSVLSFVGVLLGAIAG
                     VLLAPHIVSHIAAPRAKLFTALFLILALVVVGEVAGVVLGRAVRGAIHSRTIRSVDSI
                     IGVGVQLVVVLTAAWLLATPLTQSKDQPELAAAVRGSRVLAEVNEVAPPWLKTVPKRL
                     SALLNTSGLPAVLEPFSRTPVIPVASPDPALATNPVVTGTAPSVVKVRSLAPSCQKVL
                     EGSGFVIAPDRVMTNAHVVAGSNSVQIYASGNPLDATVVSYDPSVDIAILAVPNLPPR
                     PLTFAQAEAKTGANVVVLGYPGGGNFTATPARIREAIKLSGPDIYRDPAPVTRDVYTI
                     RASVEQGNSGGPLIDLSGQVLGVVFGAAVDDPDTGFVLTADEVAGQLAKVGATQQVAT
                     GTCVS"
     gene            complement(15436..16425)
                     /locus_tag="VFI55_04320"
     CDS             complement(15436..16425)
                     /locus_tag="VFI55_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7073294.1"
                     /translation="MAAPDPSVTRIDGPWRHLDVHANGIRFHVVEALPIGQPTGEGAI
                     MPPSEPATARPLVMLLHGFGSFWWSWRHQLRGLTGARVVAVDLRGYGGSDKPPRGYDG
                     WTLAGDTAGLIRALGHSSATLVGHADGGLACWATALLHSRLVRAIALISSPHPAALRR
                     SILTRRDQGYALLPTLLQYQAPIWPERLLTRDDAAEIERLVRSRSSAKWVASEDFSQT
                     IGYLRTAIQIPAAAHCALEYQRWAVRSQLRGEGRRFMRSMSQQLSAPLLHLRGDADPY
                     VLADPVDRTQRYAPQGRYISIAGAGHFSHEEAPAEINRHLMRFFENVHGPRVS"
     gene            complement(16426..16959)
                     /locus_tag="VFI55_04325"
     CDS             complement(16426..16959)
                     /locus_tag="VFI55_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET7073295.1"
                     /translation="MSKVDRKNGVPSTLTTIPLADPHARPADPSIGDLIKDATTQVST
                     LVRAEVELARSEITRDVKKGLTGSVYFIAALVVLFYSTFFFFFFLAELLNNWLWRWVA
                     FLIVFAIMAVVGAVLALLGFLKVRRIRGPRQTIESVRETRAALTPGHDKAPAGTKALT
                     EPGGKHESGAPADPTGW"
     gene            17369..18064
                     /locus_tag="VFI55_04330"
     CDS             17369..18064
                     /locus_tag="VFI55_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HET7073296.1"
                     /translation="MAHSWHPAAMVVVLVALMALPANVAAADDRVPMGGGAGIVINGD
                     TMCTLTTIGTDAAGDLIGFTSAHCGGPGEQVVAEAAQNRGPLGVMVSGNDNLDYAVIK
                     FDPAKVAPVATYNGFTIAGIGPDPAFGQIACKQGRTTGNSCGVTWGPGQDPGTIVMQV
                     CGQPGDSGAPVTVNNMLVGMIHGAFSDNLPTCIIKYIPLHTPAVVMSFNADLADINAK
                     HRPGAGFVPLPPN"
     gene            complement(18082..20055)
                     /gene="acs"
                     /locus_tag="VFI55_04335"
     CDS             complement(18082..20055)
                     /gene="acs"
                     /locus_tag="VFI55_04335"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HET7073297.1"
                     /translation="MTAANTGGPSSDPASYPPPTQFAEQANAGPELYREAQEDRMAFW
                     AKQANRLSWSTPFTEVLDWSGAPFAKWFADGKLNVAYNCVDRHVAAGLGDRVAIHWEG
                     EPAGDSRSLTYSDLQTQVCKAANALTGLGLVAGDRVAIYMPLIPEAVVAMLACARLGI
                     MHSVVFAGFTAKALRARIADAQAKLVITTDGQFRRGKPAPLKDAADEAIAAGPDGASS
                     VERVLVVRRTGIEVSWNDDRDLWWHDVVDSASPEHTPEPFDAEQPLFLLYTSGTTGKP
                     KGIVHTSGGYLTQASYTHYNVFDIKPDKDVFWCTADIGWVTGHTYGVYGPLSNGVSEV
                     LYEGTPDSPTQHRHFEIIEKYGVTIYYTAPTLVRTFMKWGRDIPDAHDLSSLRLLGTV
                     GEPINPEAWRWYRRVIGADSLPIVDTWWQTETGAAMISPLPGVASAKPGSAMRPLPGI
                     SAKIVDDHGDQIPPANAKGEHVSGYLVLDQPWPSMLRGIWGDPERYVETYWSRFAEQG
                     WYFAGDSAYYDADGAIWVVGRIDDVMNVSGHRLSTAEVESALVGHIAVAEAAVVGMTD
                     ETTGQAICAFVVLVADFDVHDGIVDELRVEVAREISPIARPREVHVVPELPKTRSGKI
                     MRRLLRDIAENRELGDTSTLLDPSVFDAIKTAK"
     gene            20303..>21523
                     /locus_tag="VFI55_04340"
     CDS             20303..>21523
                     /locus_tag="VFI55_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7073298.1"
                     /translation="MGSLAAIAAGWLLVACLAACLAGCGGGVLTPDLVLVNGGEPPYP
                     LIPTSTNDSNGGRILDRIFAGLMSYDAAGNPSAEVAQAIETTDNVNYRIILRPGWKFT
                     DGSPVTAHSFVDAWNYGALSTNAQLQQSFFSPIDGFDDVSGVTSEGKPAAKTGRTTMS
                     GLRVVNDLEFTVRLKEPTIDFVLRLGHSAFYPLPDIAFRDMAAFGRNPVGNGPYLLAD
                     GPDGPAWEHNVKVDLRPNPGYHGNRKPRNKGLRFELYANLDTAYADLLSGNLDVLDTI
                     PPSALPIYKRDLGDNVISGPVAVKHSLDTPLRLPHFGGEEGRLRRLALSAAVDRQHIC
                     QSIFMDTRTPSRDFTARSLPGFDPNLPGNDVLNFDPVRARQLWAQANAISKWSGQYVI
                     AYNADAGNQEWVDAV"
BASE COUNT         3586 a         7063 c         7180 g         3694 t
ORIGIN      
        1 cacctcggcc agtggacggc ggcactcgcg gtggccagcg atgagatcca acgcccgtgc
       61 ggcacactgt aaccatgagc gaaaccctga tttggatgaa gcacggcgac gattatcggg
      121 cgacgggcga cgaggaccgc gagtacttcg ttcacccgct ccctggatcg gcagagtttc
      181 cgtgggaggc ggggaccacc gccccgcggg agccgtgcaa ggagcgttcg cgggtagcgg
      241 cgcagaggct gtgtcaggcg tgggaggacg ggttggcgct gacggattag ctaccggcat
      301 gagcgacgta tcgcgcgatg gagactctgt gtcgataagc ggcccacttc ccggcttggc
      361 gcgaactgat ggacgacgac accgcatcca ctgacgtgac cgtgtggtat cgcgcagacc
      421 tattcggcat cccccctggc gaggaggcct catattccca ggactggtac gtctacgccg
      481 tcagcgattt gaaagccgcc cgcgcctttg cggccttacg agaagatcaa gagggcacac
      541 cgcgatccgt ctaccgcgta aggctcattg gggaggtgga gaccgaccct gactaccgga
      601 gtttccccga gtttgtcaga tgcctatcgg gaatagttca ggaagtcatc gaggcccacc
      661 cgacgatgtc acccgacgaa gcgaccgaat acatgtgcca gacctacaat cctccgtggc
      721 cgggtggtgg tggccccatg tacgacggcg acggataccc gacagcttcc cctgcaatgc
      781 tgggagcggg aatcagtgca aacgagcttc gccatgttgg aaaatgcaaa ccgccgctca
      841 tcattggggg ttacgcagag ggcttagtta accgggccta ggtaacctcc acccctaccc
      901 gcacgtgaac tgcggaaagc ccgcacagcg gcactcaaac ggcccgcaag tgccactacc
      961 gacagtgaat ccgcacgaga agacgtaccc ggtacgctgg gcaaacttct tagtaacgct
     1021 ctgtaagtcg tttagctgcg actacattgg tcggggtggc gggattcgaa cccacggcct
     1081 cttcgtcccg aacgaagcgc gctaccaagc tgcgccacac cccgcttgaa gccacgacag
     1141 cctatcgcac cggcccgtcg gctggcgaaa ccgcaggctg aaggccagac catcggcaat
     1201 ctcgttcgaa ttcgaccagc gtcgcggcgg ggccgctggg atcgagtccc tgcgcgctga
     1261 cccactcgtc gctgaaatag gtgtcggcgt agcggtcgcc gctgtcggcg agcagcgtga
     1321 ccaccgaacc gccgcggccg ccggcgacca tctcggccag caggccgaag gcgccccaca
     1381 ggttggtgcc cgtcgacggc cccacccggc gccccagaac ggcgctgacg tggcgagcag
     1441 cggcgataga cgccgcgtcg gggaccgcga ccatgcggtc caccacccca ggcagaaacg
     1501 acggctcgac ccgcggccgg ccgatgccct cgatgcgcga tgacgtgggc atgacgatgt
     1561 cgtatcgctc ttcggcgtag gccgggaaga acgcggagtt ctcggggtcg acgacgcaca
     1621 accgggtcgc gtgccgccgg tagcggatgt agcgcccaat ggtcgcactg gttccgccgg
     1681 tgcccgcacc gaccacgatc cattccggga cggggtactt ctcgtcgcgc atctgctcgt
     1741 agatcgactc ggcgatgttg ttgttgcccc gccagtcggt ggcgcgctcg gcgttggtga
     1801 actggtccag gtagtgccca cccgtctctc gggcgacacg ctcggcctcg gcgtacaccc
     1861 gactcgagtc cggcacgaaa tggcaacggc cgccttgcga ttcgatcagc gcaaccttgg
     1921 ccgcgctggt tgccgccggc atcacggcga cgaacggcag ccccagcagt gccgcgaagt
     1981 acgcctccga caccgccgtc gaacccgacg acgcctcgac aaccgtggtg ccctcgccga
     2041 tccagccgtt gcacagcgcg tacaggaaca acgagcgcgc cagccgatgc ttgaggctgc
     2101 cggtgatgtg cgtcgtctcg tctttgaggt acagcgcgac gtccacctcc gcgcaccagg
     2161 ccgacggcag cgggtaccga agcaggtggg tgtcggcgct gcggcgggcg tcggcctgaa
     2221 tcagccggat tgcgttatcc gtccagctgc gcggcaggct gcgggtgacg atctgggtca
     2281 gatcgttcaa cgcaccgagg cagttggctg cgaccggctc aggtccctgg aggaatcgcg
     2341 gtcgcccatc ggggtggcga tcaacgtcag cagggtcgct tccggcctgc agcagaaccg
     2401 cacgggcgca aagggagagg tacctatccc tgcggataca tacaatcgca tgttggcgcc
     2461 ccaacgggac ggtcccttgg ccctggaccg gtcgatgccg cagttggtca ccagagcgcc
     2521 gtagaacggc agacacagct gcccaccgtg ggtatgtccg gccatcacca gctggtaacc
     2581 gtcggcggcg aagcgatcca gtacccgagg ctccggggaa tgcgtcaggc ccagccgcag
     2641 gttcgcggcc gcgctggccg ggcccgcgat cgtctcgtag cggtcccggt cgatgtgcgg
     2701 gtcgtccacg cccgcggcgg cgatctgcag accggccacc tcgaactcac gccgggtgtg
     2761 ggtgaggtcg agccagccgc gctcggtgaa agccgcccgc agatcctgcc agggcagcgg
     2821 ttcgccgcgg acccggtgcg acgggttggt caggtagttc aacgggttct tcagccgcgg
     2881 cccgaaatag tcgttgctgc cgaagacgaa cacgccaggc cgcgacaaca ggtcgccgag
     2941 ggtctggacg accgcaggga cagccttggg gtgcgcaagg ttgtcgccgg tgttgaccac
     3001 caggtcgggc tcccagctga ccagttcgcg cagccaggcc tgtttgcggc gctgattcgg
     3061 cagcatgtgc agatcgctga tatgcagcac ccgcagcggt gttgagcccg gcgtcaggac
     3121 gggcatggtg atctctcgca gcacgaacgc gttgcgctcg acgagcgcgg cataaccgat
     3181 gcccgcgacg gtcgacccaa gcgcaacagc cccggtgcgt atcagggcgg gcaaaacatc
     3241 gggcatggta ggcagcctac tgccggtagc cgactcagag cgcgatctgg cgctttaaga
     3301 tcctacggag gcggtgctcc gggcggcggc ggaggtgcca gcagcggaat ggtgatcggc
     3361 ggcagccctg ggatttccac cacctgcgat ccgaccgggg gtggcggccc accctccggc
     3421 ggcggcggag gcgctggagg cacgccgttg ctgacctgga tcgtgatgat cgaccctgga
     3481 atcgtctggc cgttgggcgt cgttccgacc acctcaccaa acttggcgct gctgttgacc
     3541 gagttggttt ggtcggcaac ctggaagccc gcgtctttga ggcgctggcg tgccgcgtcc
     3601 acatccaggc ccgagacgct cggcactcgt gaggcgggcg agccgtcgac gtagcgcggg
     3661 tcggtgggcg gcaattgcac cggcccgaag ttggtggcga tcggcttcat cgcggtgaac
     3721 caggtgcgcg ccggctcgtt accgccgtac aggtcgccct cgccgcaatg ccgcagtgga
     3781 gcggagcaca aatccgtcgg cgtggtcgag tcgtcgtaga tgtagttggc ggccgcgtag
     3841 tggttggtga aaccgacaaa accggacgag cggtgcgcct cggtggtgcc tgtcttaccg
     3901 gaaaccggca ggtcccagcc ggctgcgccg gccgaaccgg ccgccgtgcc ggcgcccagc
     3961 gcgtccttac tcatcgcgtt ggccagcgtg ttcgccagac cctcgggcac cacctgatcg
     4021 caggtttcgg tggtgacggc gacttcgttg ccgttgcggt cgatcagttt gtcgatcggg
     4081 cttggcgggc accacacgcc gccggaagcc agcgtggccg cgacgttgga cagctccagc
     4141 gcgttcactt cgattgggcc gagcgtgaac gagccgatgt tctgccgctt gacgaagtcg
     4201 gccaggctct cgttgctgtc ggggttgtag tcgcgagcag tgccgggatc ggcgtaggag
     4261 cgcagcccga gtttgatggc catgtcgacg gtgcgtgtta ccccgacctg ctggatcagt
     4321 ttggcgaatg cggtgttggg cgaggtggcc agcgcatcgg tcacgttcat cgagccccgg
     4381 tagttaccgg cgttgatcac gcaccaggtg tccttggggc accccttggc cccgccgctg
     4441 cccatgccct tggcctgaaa ccggcccggg acctccagcg tggcgttgat gcccatgccc
     4501 atatccagcg ccgcggccgt ggtgaagatc ttgaagacgg accccgcgcc atcgccgacc
     4561 agggagaacg gctgcggccg catggtctcg ccggcatcga ggtccagccc gtatttacgg
     4621 ttggaggcca tcgccatgac gtggtgggag tccttgcccg gcgcgatcac gctcatcacg
     4681 ctcgagatgc ccggcagcgt cgggctggcg tacttgtcga tggccgactt gaccggagcc
     4741 tgcacgtccg ggtccaaggt ggtgcggatc gtgtagccgc ccctggctac ctgttccttg
     4801 ttgatcccgg cgcgggacag atactcctgc acgtaatcgc agaagaaggc ccggtcgccc
     4861 gccgcgatgc agccgcgcgg caactcattc ggctgcggca agatccccag cggctccgcc
     4921 ttggcggcgc gcaacgcgtc ggtctcttgc ggcaggttgt cgatcatggt gtccaggacc
     4981 aggttccgcc gggccaacgc tccgtccgga ttggtgtacg ggttgagcgt gctggtcgac
     5041 tgcaccatgc ccgccagcag ggccgcctgc tgccaattca ggtcggacgc gttgatgccg
     5101 aagtaggtct gcgcggcatc ctgcaccccg aacgagttgt tgccgaacga gaccagattg
     5161 aggtagcggg tcaagatctc gggcttgctg aacgttttgt ccagcgtgag cgccatccgg
     5221 atctcgcgca gcttgcgcgc gggggtggtt tcgacggccg cgcgcttctc ggcgtcggtt
     5281 tgggcggtga ccagcagctg gtagttcttt acatactgct gttcgatggt cgaaccgccg
     5341 cgcgtgccga cgtcgccgga cgcgtagccg gccaaccccg tcagggtgcc cttccagtcc
     5401 accccgttgt ggtcggcgaa ccgcttgtcc tcgatcgata cgatcgccag cttcattgtg
     5461 ttggcgatct tgtcggtggg tacctcgaac cggcgctgcg aatacagcca cgcgattacg
     5521 ttgcccttct tgtcgaccat cgtcgacacc gccggcacct caccctggag gagttgggcc
     5581 gagccgttgg cgacgacctc agaggcccga ttggacatca gtccaacgcc gcctaccagc
     5641 gggaacatca atgcggtggc gaccacgctg gccaacaagc agcacccggc aagcttgata
     5701 agcgtgaggg cggccggagg gcggtctgac atgcgtacta gggtagccgc ccccggtgac
     5761 ctggcgatgc cgcgagttgc gaaaaagctc cgccatgccg tgaccagcga aattgcggtc
     5821 ggacccgata taaaatttgg gtctgcgtca cggtcccgtc agcggctcga gatgcgacgt
     5881 cctacggtcg gccgtattct cggggacgag actgcacgcg cgtcccaaaa aaccgttatc
     5941 gcactgttgc gcaatcgcca cctgaccacc taacttagac agacggtgag attcagataa
     6001 caccgatgtg cacagtgtgg cgtagatcgc agcaggggtc tgcacctgag gagggcggcc
     6061 gtgagccagc ggccgggagg aagggaacag ctcgtgtcag gaacacggcc gaccgcgcgc
     6121 agaacgaaca tcgcggctgc acaaggggta ctgcacagcg tcgagaccga agagcggatt
     6181 gcatgggtct cgcaagcact gtgccggacc actgatcccg acgaactgtt tgtccggggc
     6241 gccgcccagc gcaaggcggc ggtgatctgc cgtcattgcc cggtgatgca agaatgtggc
     6301 gcggacgcgt tggacaacaa ggtcgaattc ggggtgtggg gcggcatgac cgagcgccag
     6361 cgccgggccc tgctcaagca gcaccccgag gttatttcgt gggcggactt cttcgacaag
     6421 cgccgtaacc gcagcatcgg ctgatcctgc cgttataacg atcgattcct ggcgatccct
     6481 gaagatcagc tccccatcat tacttttctg ttacagccgc ctggttcggt gagcttggtt
     6541 ggatcttgtc caatagacga tcgctcatgt tcaattcgag ctgccctgta cgcgagagaa
     6601 atccggtggt gacaatccac ccgtgccgca caggccggtc tggcgtcaaa taagagcccg
     6661 cagccagaac cggcgtgccc tcaaaacgcc gggttgtcag gtgcccgcag cccaggcggc
     6721 gtcgtcgccg acggattcgg gattggtcgg ctcctcagct gcccgcagcg cgggccgcgt
     6781 cgtcgccgac ggattcggga ttggtcggct cctcagctgc ccgatgcgcg ggccgcatcg
     6841 tcgccgaccg cggtgatctg gtccgcgagc gcgcgcagcg tgtccaagtc cgaaacgtcg
     6901 aacggcagcg acggaacccc gatgaccggc acgtgagggt tcgctccggt gaatcgggat
     6961 agcaacctga cctcccgctt ggcggtttga ccgcggtcgg catggatttt caggacggcc
     7021 gaggccagcg acgcggcgtc cgaatccttt tcggcgtcca actcctcgct tccgtcgatg
     7081 gcccgctcga ccggcagcga acacagcgtc gggtgcgtgc gattcaggac gagccccgcc
     7141 agcggcatcc cttcctgcga cagccggtcg acaaagaagg tcgcctcgcg cagcgcgtcg
     7201 ggttcggcgg ccgacaccac cacaaattgt gtaccctgct gtttgagtag cgcataagtg
     7261 cgatcggcct tttcgcggaa gccgccaaaa gtggcgtcca gtgattgcac gaatgccgct
     7321 gcatcggcca gcatctggga accgagcacg gtggagatcg ccttcatcgc caaacccatt
     7381 gcgccggtca ccaatcgccc gatgcctcga cccggcgcca gtagcagccg ccacaatcgg
     7441 ctatccatga agctgcccag tcgttttggt gcatccaaga agtccagtgc attgcgtgac
     7501 gggggagtgt ccaccaccac caggtcccac cggtcctggc tcagcagttg tccgagcttc
     7561 tccatcgcca tgtactcctg cgtgccggca agcgaagtgg cgacggtctg gtagaactgg
     7621 ttgtccagaa tcgcttgcgc ccgaccagct ccggagtatt ggaccaccat ctcgtcgaac
     7681 gtgcgacgca tgtcgagcat catcgcgtgc agctcaccgg tgacttcggg agccagcggc
     7741 acccgttgcg gggtgttgcc gaggtcgttg actcccaggg cttgcgccag tcgcttggcc
     7801 gggtcgatcg tcaaaacgca tacagtgcgc ccatattcgg ccgctcgcag cgccatcgca
     7861 gctgcggtcg tggtcttgcc cactccaccg gcgccgcagc acacgactac cctgtttgtc
     7921 ctgtcggcca gaatcgaggc catatcaagg actttcggcg tgacgctcat cgaaccccct
     7981 gttctgcaag cgattccgag agctcataca ggctgccgag atcaacaccg tcggagatcg
     8041 ccggcagttc cagtcgcgca accttgagcg cgtccagctg ttgcgcggtc tcggcgcgcg
     8101 cggcgattcg ggtcccatgc tcgatggtct cggtcagcag gccggcaaag tcggtgtcgt
     8161 tgagcttgat cccggctgtc tccagccccg cccgtatcga gtcagcgtcg acgtctccct
     8221 cggcggcctt tgccaggtcg cgcggatcga ggtacgacgg aatgttgcgg ttcacaatga
     8281 cgctgccgat cggcaactcc atctccgcga gctcctcgat ggcttccagc gtctcctgca
     8341 ccggcaacgc ttccaacaag gtgaccaaat ggatggcggt ctgctcggag tgcagcaagt
     8401 tcaccacact ctcgctctgc gaatagaccg gcccgccctt ggccagatcc gaaaccgcct
     8461 tggtgacgtc caggaaacgc gcgatccgac ccgtcggcgg cgagtcgacg acaaccgcgt
     8521 catagacggg tcgcttgtcc ttgtcgacgc gcaccaccga ttccttgatc ttgccggtga
     8581 ccagcacatc gcgcagaccg ggcgcgatcg tcgtcgcgaa ctcgatcgca ccgatgcgcc
     8641 gcatcgcccg gcccgcgatg ccgaggttgt agaacatgtc gaggtactcc aggaacgcgg
     8701 cctcgatgtc gatcgccagg gcattgacct ggccgcctcg ctcggcagtc gcaattttga
     8761 gctcctggta gggcaggggc gggacgtcga agagctgtgc aatcccttgg cgtccctcga
     8821 cttcgacgag caggactttg cggcccccag ctgccagcgt cagcgccagc gcggccgcga
     8881 cggtcgattt gcctgtgccg cccttgccgg tcacgaaatg taagcgggcc ttcgacaagc
     8941 gcgccggcca gccgacggaa ttaccgtcgc tagaagtgga tgtcaccaac gcatgctagc
     9001 tctaccgccc cacgtccacc gcagatcggg gggcggcttc aatcgggccg gccaccggac
     9061 cggagccgtc caacagcgac cgataagctc gcggcatgag ccaaccgacc gcatgggaat
     9121 acgccaccgt cccactgctg acgcatgcca ccaaacagat ccttgaccag tggggtgccg
     9181 acggctggga gctggtatcg gtgctgcccg ggccgaccgg ggaacagcac gtcgcgtact
     9241 tgaagcgccc caagtagggg gctcgggatg agcgtttcgg cccggctcga gcagcttggc
     9301 gtcgtgcttc cgcaagcggt tgcgccgctg gccgcctatg taccggcgct gcgcacaggc
     9361 aacctggtct acaccgcggg tcagctgccg atcgaaggcg gaaagctggc gcgcaccggc
     9421 aaggtcggcg ccgaagtcgg cccggaggaa gccaaggtgt tggcgcggat ctgtgcgctc
     9481 aacgcgcttg ccgcggtgga ctcgctggtg ggcatcgacg ccgtgacgca ggtggtcaaa
     9541 gtcgtcggct tcgtcgcctc cgccccgggt ttcaatggcc agcctggcgt cgtcaatggg
     9601 gcgtccgagc tgctcgccga ggtgttcggc gacaaaggag cgcatgcgcg ttcggcggtc
     9661 ggcgtctcgg agctaccact ggacgcgccg gtggaagtcg agttgatcgt ggaggtcggc
     9721 tgacggttgt gagcgaggct gtcgagtcgt tgacccatcc cgcgtacggt cggttgcggg
     9781 cagtcaccga cactgcgtcg gtgctgctgg ccgataaccc cggtttgctg acgctcgagg
     9841 gcacgaacac ctgggtgcta cgcggaccgg gcagcgacga gctggtcatc gtggaccccg
     9901 ggcccgacga cgacgaacac atcgcccgag tcgccgccgt cggtcgaatc gcgctggtgc
     9961 tgattagcca tcggcactcc gaccatacgg acggcatcga caagctggtc gagctgaccg
    10021 gcgcgacggt gcggtcagcg ggtagtgggt tcttacgcgg cctgggcagc gagctgatcg
    10081 atggcgaggt cattgatgcg gcgggcctga aaatcacagt gctggcgacc ccgggccaca
    10141 ccgcggattc gctgtcgttc gtcctcgacg acgccgtgct cacggcggat accgtgctgg
    10201 gtcgcgggac caccgtcatc gacaaggaag acggcagcct ggccgcttac ctggagtcgc
    10261 tgcggcgact gcgcggcctc ggccggcgca cggtgttgcc cgggcacggt ccggagctcg
    10321 ccgacctggg agccgtcgcg tcgggctacc tgacccaccg ggaggagcgg ctgcagcagg
    10381 tccgcgcggc actggcggac ctaggcgctg acgcgaccgc tcgccagatc gtcgagcacg
    10441 tctatgtcga cgtggatgag aaactctggg acgtcgccga atggtctgtt caggcgcaat
    10501 tggactatct gcgccgctag tgcacgccga gatcaacgcc agcgcggaga agttcgagag
    10561 gccgtgtcgg tagcgttgat ttcgacgtag acgcgctcgg ggctacctcg ctcggggttg
    10621 gattctcgct cggttgcgcc cgcgctccgc gcgcgcttgg cctgattgcc cggctcctcc
    10681 tcggtccgct tcgcggaccg catcgtcgtc gggttacctc gctcggcggg ccagccgttc
    10741 ggagtcgcaa atcagcacgc tcttgccctc cagacggatc cagccgcggt gggcgaaatc
    10801 ggccagtgcc ttattcaccg tctcccggga agcacccacc agctgcgcga tctcctcctg
    10861 ggtcaaatcg tgggtgaccc gcatcgcgcc accttcctgg gtgccgaaac gctgggcgag
    10921 ctgcagcagc tgcttggcca cccggccggg cacgtcggtg aagatgaggt cggcgagatt
    10981 gttgttggta cggcgcagcc gtcgcgcgag cacccgcagc agttgttcgg cgatctccgg
    11041 gcgatccgcg atccatgccc gcagcgcgtc gcggtccatc gacaccgcgc ggacctcggt
    11101 aatcgtggtc gcgctggaag tccgcggacc cgggtcgaag atcgacaact cgccgaacat
    11161 gtccgacggg cccatgatcg tcagcaggtt ctcccggccg tctggcgagc ggcgaccgat
    11221 cttgaccttg cccgcagtga tgatgtagag gcgatcgccc ggctctccct cggcgaaaac
    11281 cgtgtgtcct ctggggaagt cgacgggtgt cagttgcttg gtcagtgctg cgaccgcgtt
    11341 ggggtcaacc ccctggaaga ttcctgccct tgccaggacc tcgtccactt tgcctcttca
    11401 gctctcaaac gacgtgatat ggcaggctaa cccctgcccg cgtgacgagt gtagaggtaa
    11461 accagtgtgt ccaacgtgcc aacgctacac acgattggcg tgtctagtcc cccgaaactg
    11521 cggattgaca tgcgattgag tacgtattcg cgtcggtaat ccggccccat cttggtcgcc
    11581 gaaggcggtc gcaaacaacg gcgcgccgtg acgcggcgcg gtgttaggcc ccaatgccgg
    11641 ggaatcggcg agccgcccgg cctggcgccg atgcaggtat tccagagcct cgggcatgcc
    11701 ttcaagcgcc aacgtatgca tatccacggc ctcggcgtga tcgaggaact catcgacgtc
    11761 gctcgcggtg acggtgtcat gggcaagcat cttttccaga cgccccagac cgagcgtcgc
    11821 gagcatcaaa agcccgggaa cgcaggccac cagcaaccat gacacaagga gagtaaacat
    11881 ggccttgtat caacacgggg tctcggtgag attacgaaat cagtacgctg tcgtaggtga
    11941 cagcggcaaa ggcatccggg cgctcgaagt ccaagccggc ggcacccggc ggtatcgtcg
    12001 cgcggcgctt cactgaggag acccgagtcg gcttggtgcg acgggcgcgc aggatgaatc
    12061 gcgcactggc gcaagcattt ccggatgcgc actgcgagtt ggatttcacc acgccgctgg
    12121 agctgacggt ggccaccatc ctgtcggcgc agagcaccga caaacgcgtc aacctgacga
    12181 cgccggcatt attcaagcga tacccgtcgg cgctggatta cgcgaaagcc gatcgggccg
    12241 aactcgaaaa cctcatccgt cccactggtt tcttccgcaa caaggcgtct tcactcatcg
    12301 gtcttgggca ggccttggtc gaacggttcg gcggcgaggt gccgtcgaca ctcgccgaac
    12361 tggtgacgct gcccggcgtg ggacgcaaga ccgccaacgt gatcctgggc aacgcgttcg
    12421 gcgtcccggg aatcaccgtc gacacccatt tcgggcggct ggtgcgcagg tggcggtgga
    12481 ccgccgagga ggatccggtc aaggtcgagc acgccgtcgg cgaactgatc gaacgcagcg
    12541 agtggaccat gttgagccat cgagtgattt tccacggccg ccgcgtgtgc cactcccgca
    12601 aacctgcctg cggtgtctgc gtgctggcca aggattgccc gtcgttcggc ctgggtccca
    12661 ccgaaccgtt gctcgccgcg gccctcgtgc agggtcccga aaaggagcac ctgctggccc
    12721 tcgtggggct gtgatacggg tgcagcagac ttgacgccga cgacccgctg gaccattgcg
    12781 attctggcgg tggtggcagc gttgacggcg gcgctggtgg ccgagttgcg tgacgactcc
    12841 gcgacgacct cgacggtggc caccgaccag gcgccggccg cgcgcgaaca ccgcgacgcc
    12901 gacacacccg cggcgctggc gggtccccgc cagcgtgccg acctgccgcc ttgccccgcc
    12961 gccgctccgg gtttcagcgg accgggggcc gcggcgctac agggcgtgac ggccgaatgc
    13021 gccgctgacg gttcggtggt cgatgtcgcc cgcgcgctgg ccggccgccg ggtggtcctc
    13081 aatctgtggg cgtattggtg cgccccgtgc accgccgaac ttccggcgat ggccgactat
    13141 caacgacgtg tcggacccga cgtgatggtg gtgacggtgc atcaagacga gaacgagacc
    13201 gcggcattgc tgcggttggc ggagttgggc gttcggctgc cgaccctgca ggacgggcgg
    13261 cgcctgatcc cggcggcgct gcgcgtccca aacgtggtgc ccgcgacgct tgtcctgcga
    13321 ccggacggta gcgttgcgca aacgctgctg cgcgatttcg cgagtgccga cgagatcgcg
    13381 gctgcggtgg gaaacggcac gggatgaggc gaatagaggg aggcgccggt gagtgctggg
    13441 ggtactcccc tccggagcgg agaccccacc tgctcgcggg ggactccgcc tcagacgcct
    13501 ggcgtctgcc cgtcttggct gcgcccgctg gttgacaacg tcgatcagat ccctgacgca
    13561 tatcgccgtc gactgccgcc cgacgtgctg gcgatgttga ccgaggccag agccgccgcc
    13621 cagtcgtcat cgcccggcga cgaccgcgaa gcggccgtgc tggtgttgtt ctcgggaccg
    13681 gagtccggcc cggcgaacgg ccgcattccc gacgacgcgg atctgctgct gaccgtccgc
    13741 gcctcgacac tgcgccatca cgcggggcaa gcggcgttcc ccggcggcgc ggccgatccc
    13801 ggcgacgccg gcccggtggc caccgccttg cgggaggccc acgaagaaac cgggatcgac
    13861 gtcagccggc tgcatcccct ggccacgctg gcgcgcgcgt tcatcgcacc ctcgcagtac
    13921 gaggtcgtcc cggtgttggc ctattcgccg gatcccgggc cggttgccgt cgtcaacgag
    13981 acggaaactg cgatcgtgtc gcgggttccg gtgcgcgcct tcgtcaatcc ggcgaaccga
    14041 ctgatggtgt accgcggcga cctgggccgc cgttggtccg gaccggcgtt tctgttgaac
    14101 cagatgctgg tgtggggatt cactggccag gtgatctccg cggtgctcga cgtcgccggc
    14161 tgggcagtcc cctgggacac caccgacgtc cgagagctgg acgaggccat gatgctggtc
    14221 ggcgacgagg gtgggctccg atgaattcga tgaccccctc gcaatggctg gatgtcgccg
    14281 tcctggcggt cgcgttcatc gcagcaatct cgggctggcg ttcgggggcg ctgggctcgg
    14341 tgttgtcgtt cgtcggtgtg ttgctcggcg cgatcgccgg cgtgctgctg gcgccccaca
    14401 tcgtcagcca catcgccgcg ccccgcgcca aactgttcac cgcgctgttt ttgatccttg
    14461 cgctggtcgt cgtcggtgag gtcgccggtg tggtgctggg gcgtgccgtt cgcggcgcga
    14521 tccacagccg caccatccga tccgtcgact cgatcatcgg ggtgggggtc cagctcgtcg
    14581 tggtgttgac cgcggcctgg ttgttggcga cgccgctcac acagtccaag gaccagcccg
    14641 agctggcggc cgcggtccgg ggttcccgag tgctcgccga ggtcaacgag gtcgcaccgc
    14701 cctggctgaa aacggtgccc aagcggctgt cggcgctgct gaacacctcg ggcctgcccg
    14761 cggtcctgga gccgttcagc cgtactcccg tgatcccggt cgcctcaccg gatcccgccc
    14821 tggccaccaa tccggtggtg acgggcaccg cgcccagtgt cgtcaaggtg cgcagcctgg
    14881 cgccgagctg ccaaaaagtt ttggagggca gcggattcgt gatcgcaccc gatcgggtga
    14941 tgaccaacgc gcacgtggta gccgggtcca acagcgtcca gatctacgcc agcggcaacc
    15001 ccctggatgc caccgtggtg tcctacgacc cgtcggtcga catcgccatt ctcgccgtcc
    15061 cgaacctgcc cccgcggccg ctgaccttcg cccaggccga ggcgaagacc ggcgcaaacg
    15121 ttgtggtgct gggttatccc ggtggcggca acttcaccgc gaccccggcc aggattcgcg
    15181 aggccatcaa gctcagtggt cccgacatct accgcgaccc ggcaccggtc acccgcgacg
    15241 tgtacaccat tagagccagt gtggagcaag gcaactcggg tggaccgttg atcgacctca
    15301 gcggtcaggt gctcggtgtg gtgttcggtg cggctgtcga cgaccccgac accggtttcg
    15361 tgttgaccgc cgacgaggtg gccggtcagc ttgccaaggt cggtgccacg cagcaggtgg
    15421 ccaccgggac ctgcgtcagc tgacccgggg tccgtgcacg ttctcgaaga acctcatcag
    15481 gtgcctgttg atttccgctg gtgcctcttc gtggctgaaa tgtcctgcgc cggcgatgga
    15541 tatgtagcgc ccctgtggtg cgtaacgctg ggtgcgatcg acggggtcgg ccagcacgta
    15601 ggggtcggcg tcaccgcgca agtgcagcaa cggcgcactg agctgttggg acattgatct
    15661 catgaatcgc cggccctcgc cgcgcagctg gcttcgcacc gcccagcgct ggtattccaa
    15721 tgcgcagtgc gcggcggccg gaatctggat cgccgtgcgc agatagccga tggtttggga
    15781 aaaatcttcg gaagcaaccc atttggcact actgcggctc cggaccaggc gctcgatctc
    15841 cgccgcgtcg tcccgggtca gcaggcgctc gggccagatc ggcgcttgat attgcagcag
    15901 cgtcggcaac agcgcgtatc cctggtcgcg ccgcgtcagg atggatcgtc gcagcgccgc
    15961 cgggtgaggc gaactgatca gcgcgatggc gcgcaccagc cgcgaatgca gcagcgcggt
    16021 ggcccagcag gcgagcccgc cgtcggcgtg gccgaccagc gtcgccgacg agtgcccgag
    16081 cgcacgaatg agccctgcgg tatcgccggc cagcgtccag ccgtcgtagc cgcgcggggg
    16141 cttgtcgctg ccgccgtagc ctcgcagatc gaccgcgacc acccgcgcgc cggtcagccc
    16201 gcgcagctgg tgacgccagg accaccagaa cgacccgaaa ccgtgcagca acatcaccaa
    16261 tggccgcgcc gtcgccggct ccgagggcgg cataatcgcg ccttcgccgg tcggctggcc
    16321 aatcggcaaa gcctcgacga catgaaatcg gatgccgttg gcgtgcacgt ccagatggcg
    16381 ccatgggccg tcgatgcggg tgaccgacgg atctggtgct gccatctacc aacccgtggg
    16441 atcggcgggg gcgccgcttt cgtgcttgcc gccgggctcg gtgagcgcct tcgtcccggc
    16501 gggggccttg tcatggccag gggtgagcgc ggcgcgcgtc tcccggaccg attcgatcgt
    16561 ctgccgcggt ccccggattc ggcgcacctt caagaaaccc aacagggcca gcacggcccc
    16621 gaccaccgcc atgatcgcga acacgatcag gaacgccacc cagcgccaca gccagttgtt
    16681 gagcagctcg gcgagaaaga agaagaaaaa gaacgtggag tagaacagca ccaccagcgc
    16741 ggcgatgaag tagacgctgc cggtgaggcc cttcttgacg tcccgggtga tctcggagcg
    16801 ggccagttcg acctcagcgc gcaccagagt ggatacctgg gtcgtcgcgt ctttgatcag
    16861 atcgccgatc gacgggtccg cgggcctcgc atgcgggtcg gccaacggga tcgtggtcag
    16921 ggtgctgggc acaccgttct tgcgatcgac tttgctcaca gacggtcctc tccgttcggt
    16981 tgcccggggc ctcatcgagc cgttgctcgg cccgcagcgt cgccgagcct gttcgctcgt
    17041 cgcggttcat gttgccatgc ggtgcggtgc cagacgaggg cagccgaaaa tcgaggcaca
    17101 tctcggcggt cgggtggatg cggcgcgctc tggccggcgg ccgaaaaagt tgagccgcaa
    17161 gggcttagcc ttcgcctgcg cggccgccgc ccgcatgtgg tcagttccgg ttcacgggga
    17221 ctcagagcgc cgatcgtgtt gggagcgaac agaataaaca aagccgcgtg gggacggcgc
    17281 cgaccagcgc gaacgagatg cgttatccga ttagactggc cacgctgctc gggatgttcg
    17341 gcccgttgag gggagtgttg ctttgcagat ggcccacagt tggcatccgg cggcaatggt
    17401 agtcgtgctc gtggcgctga tggccttgcc cgccaacgtc gccgcggctg acgacagggt
    17461 cccgatgggc ggcggtgcgg gcatcgtcat caacggggac accatgtgca ccctgacgac
    17521 gatcggcacc gacgcggcgg gggatctgat cggattcacc tcggcgcact gtggcggtcc
    17581 cggtgagcag gtcgtcgcgg aggccgccca aaacaggggc ccgctggggg tcatggtctc
    17641 gggcaacgac aacctcgact atgcggtaat caagtttgac ccggccaagg tggcaccggt
    17701 ggccacgtac aacggattta ccatcgccgg catcggtccg gatccggcct tcggtcagat
    17761 cgcctgcaag cagggccgca cgaccggcaa ttcgtgcggc gtcacctggg ggcccggcca
    17821 ggacccgggc accatcgtca tgcaggtgtg cggtcaaccg ggtgactccg gggcgccggt
    17881 gaccgtcaac aacatgctgg tggggatgat tcacggagcg ttcagcgaca acctgccgac
    17941 gtgcatcatc aaatacatcc cgctacacac cccggcggtg gtgatgtcgt tcaacgccga
    18001 tctcgccgac atcaacgcca aacaccggcc cggcgcggga ttcgtgccgc tgccgcccaa
    18061 ctgagcaacg cgtcgcgcga cctactttgc cgtcttgatc gcgtcgaaca cgctgggatc
    18121 cagcagcgtc gacgtgtcac ccaactcgcg gttctccgcg atgtcgcgca acagccgccg
    18181 catgatcttg ccgctgcggg tcttcggcag ctcgggcacc acatgaacct ctcgcggtcg
    18241 cgcgatgggt gagatctccc gggccacctc gactcgcaac tcgtcgacga tcccgtcgtg
    18301 tacgtcgaag tcggcgacca ggacgacgaa cgcgcagatg gcctggccgg tggtttcgtc
    18361 cgtcatcccg accaccgccg cctcggccac cgcgatgtgg ccgacgaggg ccgattccac
    18421 ctcggcggtc gagagccgat gccccgacac gttcatcacg tcgtcgatgc ggcccaccac
    18481 ccagatggcg ccgtccgcgt cgtaataggc gctgtcgccg gcgaagtacc agccctgctc
    18541 ggcgaaccta gaccagtaag tctcgacata gcgttccgga tcgccccaga tgccgcgcag
    18601 catggacggc cacggctggt ccaggaccag gtagccgctg acgtgctcgc ccttagcgtt
    18661 tgccggcggg atctgatcgc cgtggtcgtc gacgattttg gccgagatgc ccggcaacgg
    18721 cctcatcgcc gaacccggtt tggccgaggc gactccgggc agcggggaga tcatcgcggc
    18781 gccggtttcg gtctgccacc aggtgtccac gatcgggagg ctgtcggcgc cgatcaccct
    18841 gcggtaccag cgccacgctt cggggttgat cggctcgccg accgtcccca gcagtcgcag
    18901 gctggacagg tcgtgcgcgt cggggatgtc gcgcccccac ttcatgaacg tccggacgag
    18961 cgtgggcgcg gtgtaataga ttgtcacgcc gtacttttcg atgatctcga aatgccggtg
    19021 ctgagtgggc gaatcggggg tgccctcgta gagaacctcg gagaccccgt tggacagcgg
    19081 accgtagaca ccgtaggtgt gtccggtgac ccagccgatg tcggcggtgc accagaacac
    19141 gtccttgtct ggcttgatgt cgaagacgtt gtagtgcgtg taggaggcct gggtcagata
    19201 gccgccgctg gtgtggacga tgcccttggg cttgccggtg gtccccgagg tgtacagcag
    19261 gaacagcggc tgctcggcgt cgaacggctc cggggtgtgt tcgggcgatg cggaatcgac
    19321 gacatcgtgc caccacaggt cgcggtcgtc gttccaagac acctcaattc cggtgcgccg
    19381 caccaccaga acgcgctcga cggagctggc gccgtcggga cccgctgcga tagcctcgtc
    19441 ggccgcgtcc ttgagcggtg ccggcttgcc acggcggaac tgcccgtcgg tggtgatcac
    19501 cagcttggcc tgggcgtcgg cgatccgggc gcgcaatgcc ttggcggtaa acccggcgaa
    19561 cacgacgctg tgcatgatgc cgaggcgcgc gcaggcaagc atcgcgacga ccgcttcggg
    19621 gatcagcggc atgtagatcg cgacccgatc gccggcgacc agacccagcc cggtcagcgc
    19681 gttggccgct ttgcacacct gggtctgcag atcggagtag gtcaggctgc ggctgtcgcc
    19741 ggcgggctcg ccctcccagt gaatcgcgac ccgatccccg agaccggccg ccacgtggcg
    19801 atccacgcag ttataggcga cgttgagctt gccgtcggcg aaccacttgg cgaacggcgc
    19861 ccccgaccag tccaatacct cggtgaacgg cgtcgaccag gacagtcgat tggcctgctt
    19921 ggcccagaag gccatccggt cctcttgcgc ctcgcgatac agctccggac cggcgttggc
    19981 ctgttcggca aactgcgtcg gcggcgggta cgaagcggga tccgaagacg ggcctccggt
    20041 attggcggcg gtcactggct tcctcctagt gtcgaggctc gtcaaccgct tgagagccta
    20101 accccgcgag ccgcggagca taatcaccgg ttatccggtc gtcgactggg gcaccggcgg
    20161 gccgccgctt cacgaccggt ggaaggaccc cggcaggggc cgtgattttc cgatcggtgc
    20221 agggggcggg tgtctggtag tggatcaccg caccctgcag gtcgggcagc ccgcacgccc
    20281 tatcgtcgct gacatgcatc ggatgggctc gctggccgca atcgccgccg gatggctgct
    20341 ggttgcgtgc ctggccgcct gccttgctgg ttgtggaggc ggcgtcttga cccccgacct
    20401 ggtgctggtc aacggtgggg aaccgcccta cccgctgatc ccgacgagta ccaacgacag
    20461 caatgggggg aggatcctcg atcggatctt cgccggactc atgtcctacg acgccgccgg
    20521 aaacccttcg gccgaggtcg cccaggcgat cgagaccacc gacaatgtca actaccgcat
    20581 cattctgcgg cccggctgga agttcaccga cggctcgccg gtcacggccc attcgttcgt
    20641 cgacgcgtgg aactacggag ccttgagcac caatgcccag ctgcagcaga gcttcttcag
    20701 cccgatcgac gggttcgacg acgtctccgg ggtgaccagc gaaggcaagc ccgccgcaaa
    20761 aacgggacgg accaccatgt ctggtctgcg ggtggtcaac gacctcgagt tcaccgtgcg
    20821 gctcaaggag ccgaccatcg acttcgtctt gcgactgggc cacagcgcct tttatccgct
    20881 gccggacatt gcatttcgcg acatggcggc gttcggccgc aaccccgtcg gcaacggccc
    20941 gtacctgctc gccgacggcc cggacgggcc cgcctgggaa cacaacgtca aggtcgacct
    21001 gcggcccaac cctggctatc acggcaaccg aaagccccgc aataaaggcc tgaggttcga
    21061 gttatacgcc aatctggaca ccgcctacgc cgatctactg tcgggcaatc tcgatgtgct
    21121 ggatacgatt ccgccgagcg cgctgccgat ctacaagcgc gacctgggcg acaacgtcat
    21181 cagcggcccc gtggcggtca aacacagcct cgatacgccc ctgcggctgc cgcatttcgg
    21241 cggcgaggag gggcggctgc gccggcttgc gttgtcggcc gccgtcgacc gtcagcacat
    21301 ctgtcagagc atcttcatgg acactcgcac cccatcgcgc gatttcaccg ccaggtcgtt
    21361 gccgggtttc gatccgaacc tgccgggcaa cgacgtattg aacttcgatc ccgttcgtgc
    21421 gcgccaactt tgggcgcaag ctaacgcgat ctcgaagtgg agcgggcaat acgtcatcgc
    21481 ctacaacgcg gacgccggaa accaggaatg ggtggacgcg gtg
//
LOCUS       DASSGQ010000095         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_583705,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000095 DASSGQ010000000
VERSION     DASSGQ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583705"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="VFI55_04345"
     CDS             <1..863
                     /locus_tag="VFI55_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7073299.1"
                     /translation="VRTFNKAFAPYGLPRTAFKPSYGIAEATLFVATIEPSAQASVVY
                     FDREQLGAGHAVRVPEGTPSAVAQVSCGRTARSLWAVIVDPDTGTELPDGDVGEIWLQ
                     GENVGRGYWGLPEETQLAFGAKLQTRLGTGSHTEASTVEGTWLRTGDLGVYLDGELYV
                     TGRIADLVIIDGRNHYPQDIEATVAEASPMVRRGYVTAFSVPADRVPGSGAGNADTGE
                     RLVIIAERAAGTSRDDPLPAIEAIRAAVSQRHGVAIADVRFLPAGAIPRTTSGKLARR
                     AARAEYLSGT"
     gene            923..1603
                     /locus_tag="VFI55_04350"
     CDS             923..1603
                     /locus_tag="VFI55_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073300.1"
                     /translation="MGLLFRLAELLIMILPLVGVIIAGVKAFGAYQRRAQEPQGVDRA
                     STPDAVIPPTAHAGNSQAAQWRSLRRVIDEHGRTDARWLEYELDFAKLLDFPLMTDMR
                     DPLTIGFHKAKLRADFLRPVKAEELLDDRDAAAQYRAAVEDYVTAFNTAEAEAIRRRR
                     SDFSTAGRQRIARAQSLLRVASDPAATPQERQNAYDLARKELDGIVVLPSTTRASIER
                     GISGQFEG"
     gene            complement(1851..2330)
                     /locus_tag="VFI55_04355"
     CDS             complement(1851..2330)
                     /locus_tag="VFI55_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MPT63 family protein"
                     /protein_id="HET7073301.1"
                     /translation="MKITTMMKTAIAMVGMATIAIAGAPIATAVYPMNGKFGSELTMT
                     DTVGQVVLGWTVNDLKSSADTIPNYPVAGQLWEATATVNAIRGTVTPAISQFNARTAS
                     GTDYRVLWQASGPNTISGATIPQGAQSTGKIYFDVTGPAPTVVTMNNGMEDLMIWGP"
     gene            2526..>3056
                     /locus_tag="VFI55_04360"
     CDS             2526..>3056
                     /locus_tag="VFI55_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HET7073302.1"
                     /translation="MTAIPGPPAADSGGSPALAGFPVVAPPLRGPVTFEQRWVDLTFL
                     HWPVRPASVEGLYPPGTRPDVFDDGMTYVALVPFAMNATTVGTTLPLPYFGSFAETNV
                     RLYSIDDAGRHGVLFRSLDAARLTAAAAIRIGLGVPYAWSRMRMTRRGDHITYDSVRR
                     CPRRGLRSRLTVALGDA"
BASE COUNT          504 a         1012 c         1009 g          531 t
ORIGIN      
        1 cggtcaggac gttcaacaag gcgttcgcgc cgtacggatt gccgcgcacc gcgttcaaac
       61 cctcgtacgg catcgcggag gcgacgctgt tcgtcgcgac catcgagccg tccgctcagg
      121 catcggttgt ctatttcgac cgcgaacaat tgggcgccgg tcacgcggta cgcgtccccg
      181 aaggcacccc ctccgcggtg gcgcaggtgt cctgcggccg gacggctcgc agcctgtggg
      241 cggtcatcgt ggaccccgat accgggaccg aactgcccga cggtgatgtc ggcgaaatct
      301 ggttgcaggg cgagaacgtc ggccggggtt attggggact gcccgaggaa acccagcttg
      361 cgttcggcgc caagctgcag acccggcttg gcacgggcag ccacaccgag gcctcgaccg
      421 tcgaaggcac ctggctgcgg accggcgacc tcggggtgta tctggacggc gagctctacg
      481 taaccggccg gatcgcggat ctggtgatca tcgacggccg caaccactac ccgcaagaca
      541 tcgaggccac ggtcgcggaa gcgtcgccga tggtgcggcg cggatacgtg accgcgttct
      601 ccgtgccggc cgaccgggtg ccgggctcag gcgccggcaa tgccgacacc ggcgaacgcc
      661 tggtgatcat cgccgaacgt gccgccggca ccagccgcga cgatccgctg ccggccatcg
      721 aggcgatccg ggccgcggtg tcgcaacggc acggtgtggc catcgccgac gtgcgcttcc
      781 tgcccgccgg tgcgatcccg cgcaccacca gcggcaagct ggcccgccgg gccgcgcgcg
      841 ccgagtacct cagcggtaca taaccggcgg cactgcccgc caccgggccc gcgcaccgtc
      901 gcagtggtgg catcctggcc gtgtgggttt gctattccgc ttggcggagc tgctgatcat
      961 gatcctgccg ctggtgggtg tgatcatcgc gggggtaaag gcattcggcg cctaccagcg
     1021 gcgagcccag gagccgcagg gcgtcgatcg ggcttcgaca ccggacgcag tgatcccgcc
     1081 caccgcgcac gcgggcaata gtcaagcggc gcaatggcgt tcgctgcgac gcgtcatcga
     1141 cgagcacggt cgtaccgacg cccgctggct cgagtacgaa ctcgacttcg ccaagcttct
     1201 cgacttcccg ctcatgaccg acatgcgcga tccgctcacg atcggcttcc acaaggccaa
     1261 actgcgggcg gacttcctgc gcccggtcaa ggccgaagaa ctcctcgacg accgggacgc
     1321 cgcggcgcag taccgcgccg cggtcgagga ctatgtgacc gcgttcaaca ccgccgaggc
     1381 ggaagcgata cgaaggcgcc gcagcgactt ctcgacagcg ggccggcagc gaatcgcgcg
     1441 agctcagagc ctgctgcggg tggcgtcgga ccccgccgcg acgccgcagg agcgtcagaa
     1501 tgcctacgac ctggcccgca aagagctcga cggcatcgtc gtgttgccat cgacgacgcg
     1561 ggccagcatc gaacgtggga tctcgggcca gttcgagggc tgagctatgg gttcttattc
     1621 gcgcatgcgc gatctggccc ggtaaaaagg acatcaaaag gtcgccgtga ctggcatgtc
     1681 gcgggtgtgg ccgaatatct ggatcgcgct ttacgccagt caagattccg cattttcccg
     1741 atggtcacga cgcgaacgcg acggcgcgtt attgacggcc tgtaattcat tgagccgcaa
     1801 ggtgaatcgc ggcccgcatt aggggggatg atctagcgtg ctgaggccct ctaaggcccc
     1861 caaatcataa ggtcttccat gccattgttc atggtgacca cggtcggcgc cggaccggtg
     1921 acatcgaagt agattttgcc ggtcgattgc gcgccctgcg ggatagtggc cccgctgatg
     1981 gtgttcggac ccgaggcctg ccacaagacc cggtagtcgg tgccactggc ggtacgggca
     2041 ttgaactgag agatggcggg agtgacggtg ccccggatcg cgttgacggt cgcggtggct
     2101 tcccagagct ggccggcaac cggataattc gggatggtgt cggcgctgga tttcaggtcg
     2161 ttgaccgtcc agccgaggac aacttgtcca accgtgtccg tcattgtcag ctcgctgccg
     2221 aatttgccgt tcatgggata gacggccgtc gcgatcggag cgcccgcgat agcaatcgtg
     2281 gccatgccca ccatcgctat cgccgtcttc atcattgtgg tgattttcat tggtcctccg
     2341 ctcctctcgt ttgagcggcc agaccattcg aagccgcccg acgtaataca tactttaagc
     2401 cgcaaacgac caaatgtttc caaagaattt gacgccaagc ggccggtgtg cggcttcttt
     2461 gtcgacgatt cggacttctc gtgatggaga gtcggcgtgc ccgatccttg tgccacgatg
     2521 gtcggatgac cgccataccc ggcccgccgg ccgcggattc aggcggcagc ccggctcttg
     2581 ccgggttccc ggtcgtcgca ccacccctgc gcggcccggt taccttcgag cagcgctggg
     2641 tcgatctgac cttcctccat tggccggtgc ggccggcaag cgtcgaaggc ctgtacccgc
     2701 cgggaacccg cccggacgtc ttcgacgacg ggatgaccta cgtggcgctt gtcccgtttg
     2761 cgatgaacgc caccacggtt ggcaccacgc ttccgctgcc gtatttcggc agcttcgcgg
     2821 agacgaacgt ccggctttat tcgatcgacg acgccggacg acacggcgtc ctcttccgat
     2881 cgctggacgc ggctcggctg accgcggcgg ccgccatccg gatcggtctc ggcgtgccct
     2941 acgcctggtc gaggatgcgg atgacgcgac gcggcgacca catcacgtat gacagcgtgc
     3001 gtcgctgccc gcgccgcggc ctgcgcagca ggctgacggt cgccctgggt gacgcg
//
LOCUS       DASSGQ010000096        20291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1161614,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000096 DASSGQ010000000
VERSION     DASSGQ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 20291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20291
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161614"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VFI55_04365"
     CDS             complement(<1..733)
                     /locus_tag="VFI55_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017487610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B synthase"
                     /protein_id="HET7073303.1"
                     /translation="MVRARDTDACPGALQVHWAADGALVRVRLPGGAITAEQMATLSR
                     VSTDLGSGRLELTARGNVQLRGITDVTAVAQAIAEVGLLPSPTHERARNIVASPLSGR
                     TGGRADVRPWVSELDAAICADPRLAELGGRFWFSIDDGRADVSGLCADVGVHVLDDGV
                     ALLLAGRDTGMRLAVDDIAESLVALAARFIEIRGNAWRVNELADIGSLLPGTDISATT
                     FPPVTRPPVGWISQRDGRIALGAALP"
     gene            908..3511
                     /locus_tag="VFI55_04370"
     CDS             908..3511
                     /locus_tag="VFI55_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET7073304.1"
                     /translation="MVGRDVELGQALAAFEAGPPLRGVALLGESGVGKSTLARTLAET
                     LASGNHTVRFVLGTETGRAIPLGAFYRSVTVDATHEPAIMLASAHQALEREENLVIVV
                     DDAQLLDPLSATLVHQLAVSGQTGLIVVVRTGDSAPDAVTALWKEQLLLPLSIKPFTR
                     KQVGELARGVLGGEVDGRLIDELHQRTAGNLLLLRGLLSAGRESGVLARTETGWRLHG
                     PLRGDDELYDLVEFRLRSLAPEELEVIEVVALAEVLDWHILQAVCDAYAVARLERRGL
                     IQLVADGAHAVARLNHPVIGEAALQRAGVARTRQLNTLLAQQLQQRSAHLPAPDVRTR
                     IQLAQFMIQSDLAPDLEVIMDAAASAVTMSNVAFGEKLARFAFQRGGGLRAAIVLAEA
                     VSWQGRGDEAEDVLSAFDPDGSDELLTVRWGCLRAANLFWGCGRVDAARAVLANVKDR
                     VNSGAMLGLAAAMGVSFAFFSGDLSTAMTAGLVVCESGVGPPAAVWAAMSTSWAMALS
                     GRFPDCHRVANAGFRAAALGESGPQRFAIGLAEVMALTAAGDLPAADLVCERYAAAAA
                     GVREAEAIVKALVGLANFARGALGPACEALRDSVAAMLTGFPSGWLMLVSALLAQAEA
                     GQQHDDDAGSALKLSEDANGPQVAVFLPELELARGWVRASCGQTSSAQRHALRAAEIA
                     RQSGMCAVEMRALHTAVRFGDRAQAARLSELARILDAALPDVIAAHARALADHDGGLL
                     DDVADRFSGMGAMALAADAAAQAAREHARAGERAKELESSARVHWLAGKFGLQSPAID
                     AATQPLPITDREREIAAMVSAGLSNRAIADRLSVSVRTVDGHLYRIFAKLDIKNRDQL
                     ARLLNPAESHA"
     gene            3524..4033
                     /locus_tag="VFI55_04375"
     CDS             3524..4033
                     /locus_tag="VFI55_04375"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET7073305.1"
                     /translation="MCSEALRIVREYRTCEFTTVSGNGAPQTWPVSPLLLEDGRFLIA
                     TNIGLPQKALNVRRNPKVSLLFSDPTGSGIARPGAVLIQGDAICEERIVSDIAREPDL
                     AALVQTVFGRQPTGSSFMISRLGRRLFPFYCLRLLIHVLPRRAHFWPTRDFAGPPEQI
                     DPRDLQHVV"
     gene            4023..4481
                     /locus_tag="VFI55_04380"
     CDS             4023..4481
                     /locus_tag="VFI55_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073306.1"
                     /translation="MWSDAASELAGYPDAVLTALDPAGYPVSVRQRAPRYDPATGQCT
                     VSWPSELPVAAGPAVLLCHRHDENLWNIRQMHIEGRLERRADQWIFTSTDFRRPPHSQ
                     LGVFWRLARDMRRAGRRYLDRRGLKTPTVNWKALEALAITRQQNSGSRLL"
     gene            4545..5966
                     /locus_tag="VFI55_04385"
     CDS             4545..5966
                     /locus_tag="VFI55_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7073307.1"
                     /translation="MDKRGGHAVVLGASMGGLLAARVLADFYGRVTVVERDILPRDAV
                     NRRGVPQGRLIHAALAQCTGILDELFPGFVEDLKAAGVGSWDDGDLSKLWMSVGSHQA
                     VRSGRAPNAPRILFPSRPVLEWNVRRRVQAIDNVMFLEGHDVLALTATANRGRVTGAK
                     VVDRANDRESTLTADLVVDATGRGSRTPFFLEGLGYGRPREDELIVQLEYACQLLRLT
                     PDAVHEYMIALFPKPGRPKMFGLIRYENDTWMFGVGTMAGAKPPSRRDDMVTFAADFA
                     PAHAMEAIRTAEPLGEVVHHRVPSNRWRRYDKMRRTPDGLLVVGDAVCSFNPIYGQGM
                     TVAAIEAMVLRDCLRRGDRGLPRRFFRASARKVRVAWQTAVGSDLNLPEVVGPRPMSM
                     RISNSFLERVLSAVEVDTVVAGQFMRVTAMVDPPARLLRPSILLRLMRTSGRRATAAG
                     SVDEIASRTDGPRTGIAMTQTGS"
     gene            5991..6377
                     /locus_tag="VFI55_04390"
     CDS             5991..6377
                     /locus_tag="VFI55_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7073308.1"
                     /translation="MSNQNIETTKKGYAAFAAGDLETALSVFDDSVEWTINGQSTIGG
                     TYHGKNELTDLLMRLSEKTTKVETKRFLADGDVVIALTEVTAGAETGPEADVFEFRDG
                     KVVRALSLGDTALQERVFGSKRVAAG"
     gene            6457..10032
                     /gene="cobN"
                     /locus_tag="VFI55_04395"
     CDS             6457..10032
                     /gene="cobN"
                     /locus_tag="VFI55_04395"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HET7073309.1"
                     /translation="MAEPTILLLSTSDTDLISARASGKNYRWANPSRLTDDQLPDLLA
                     NAAIVVVRILGGYRAWQSGIDAVIASGVPTVLVSGEQAADAELTGLSTPAAGIAVQAH
                     IYLAHGGVDNLRQLHAFLSDTVLMTGFGFAPPVVTPTWGELERPLASHTEGPTIAVLY
                     YRAQQLAGNTAYIESLCRAVEDAGGRALPVYCASLRTAEPELLQRLGEADAMVVTVLA
                     AGGLRPATVSAGGDDDSWNVEHLAALDIPILQGLCLTSPRRQWLDNDDGLSPLDVASQ
                     VAVPEFDGRIITVPFSFKEIDDDGLIAYVADPERCARVAGLAIRHAQLRHVAPADKRV
                     ALVFSAYPTKHARIGNAVGLDTPASAVALLRAMRERGYDVGDLPGVDAADGDALIHAL
                     IERGGQDPDWLTDGQLAGNPVRMSAKDYRDWFATLPAELTDAVQAHWGPPPGGLFVDR
                     SNDPDGEIVIAAMRAGNVVLMVQPPRGFGENPVAIYHDPDLPPSHHYLAAYHWLDVGF
                     GSHALVHLGKHGNLEWLPGKTLGMSAACGSDAALGNLPMIYPFLVNDPGEGTQAKRRA
                     HAVLVDHLIPPMARAETYGDIARLEQLLDEHAAVAALDPGKLPAIRQQIWTLIRAAKM
                     DHDLGLTERPEEDSFDDMILHVDGWLCEIKDVQIRDGLHILGQKPTGEAELDLVLAIL
                     RARQLFGGEHVLPGLRQALGLAEDGTDERSSVDHAEAAARELVAALQASGWDPDGVQR
                     LTDDADVAAVLRFAATEVVPRLAGTAAEIDQVLRALDGRFIPAGPSGSPLRGLVNVLP
                     TGRNFYSVDPKAVPSRLAWEAGVALADSLLARYRDDHGQWPQSVGLSVWGTSAMRTAG
                     DDIAEVLALLGVRPVWDDASRRVVGLTPIPLTELGRPRIDVTVRISGFFRDAFPHVVT
                     MLDDAVRLVADLDEPADDNYVRAHAQADLAQHGDQRRSTTRIFGSKPGTYGAGLLQLI
                     DSCNWRDDADLAAVYTAWGGFAYGRDLDGREAVDDMNRQYRRIAVAAKNTDTREHDIA
                     DSDDYFQYHGGMVATVRALTGQAPAAYIGDNTRPDAIRTRTLSEETARVFRARVINPR
                     WMAAMRRHGYKGAFEMAATVDYLFGYDATAGVMADWMYEQLTERYVLDAENRKFMTES
                     NPWALHGMAERLLEAAGRGMWAEPQPDTLDGLRQALLETEGDLEG"
     gene            10122..10502
                     /locus_tag="VFI55_04400"
     CDS             10122..10502
                     /locus_tag="VFI55_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET7073310.1"
                     /translation="MTPTFADLAKAQYILLTTFTKDGRPKPVPIWAAADGDRLLVITE
                     AKSWKVKRIRNTPRVTMATCTMRGRPTSEAVEGTAAILDKSQTGTVYDAIGRRYGIVG
                     TVFNFVSKLRGGMQNNVGLELRVA"
     gene            10527..11387
                     /locus_tag="VFI55_04405"
     CDS             10527..11387
                     /locus_tag="VFI55_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET7073311.1"
                     /translation="MGAVLVPIPDRDFDPTEVAVSWRVLTRNGHRVVFATESGDPGVA
                     DDIMVTGRGLDVWSELPGLSAIPLLGLMLRANKDGRDAYQEMLRSAEYQRPVSWAQAT
                     LDGVDALLLPGGHRARGMRSYIDSDVLHRLVVDAFARGLIVAAICHGVLLAARSVDPG
                     TGRSVLYGRKTTALTWAMERLAWRLTRITRYWDRNYYRTYAEEPGQPSGYMSVQSEVT
                     RALEDPADFCDVVRGSPDWLLKSSGMVRDTFTDSRPAFVVDDGSYLSARWPGDVHTFA
                     GALSRKLPAG"
     gene            complement(11412..12032)
                     /locus_tag="VFI55_04410"
     CDS             complement(11412..12032)
                     /locus_tag="VFI55_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073312.1"
                     /translation="MRGWAIRRHARWVDVGVALVVLAVLIVVGRVAMFGRDDAPSSQP
                     SRGAVRDAATQTVPRARFSGAEQQIVGLLPPGYSAAACTRAVNPFPSAVASLDCSQNT
                     NSDTPTYARFTLYNDMDALTGDFQLTADGMAVSACPGENTSRGTWSYRADAAQISGKI
                     VCGIVADRANIAWTRDDQLLLATVNGGPDLTSLYRWWRTYGGMPQR"
     gene            complement(12157..12863)
                     /locus_tag="VFI55_04415"
                     /pseudo
     CDS             complement(12157..12863)
                     /locus_tag="VFI55_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770891.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
     gene            12937..13479
                     /locus_tag="VFI55_04420"
     CDS             12937..13479
                     /locus_tag="VFI55_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HET7073313.1"
                     /translation="MLRRLLLIYAIVELAAFFLLISTIGWGWALMALLVSFVVGWGLL
                     APMAGSHLIGQIGRLRSAPAEPNRAVSDGALVTLATTLVLVPGVVTTALGLLLLIPPI
                     RSVARPGLTALGMRGLRRRMPLLTDATLFGAGARRGYAGDGRDYIDGEVIDVLDVEPP
                     SLAGDPMGHEFPGRPASSTS"
     gene            13607..15139
                     /locus_tag="VFI55_04425"
     CDS             13607..15139
                     /locus_tag="VFI55_04425"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET7073314.1"
                     /translation="MAVRGGIVAWLGSDDVGRSQFPDADVEDLDGGFVAPGFVDSHLH
                     LTATGLTLSGLDLRPAVSRAQCLQMVADYAAAHPGRPVWGHGWDESSWPENTPPSSGD
                     LDAVLGDRPAYLARVDVHSALASTALRRLVADLPAAAGFAPQRPLVGDAHHLTRAVAR
                     DLLTAEQLAEARTVALDAAAAAGIVAVHECAGPEIGGLDDWLRLRDLNHGVEVIGYWG
                     EAVATPAQARSLVDATGAHGLAGDLFVDGALGSRTAWLHEPYRDAPDRLGSCYLDHAA
                     IEAHLRACTEAEVTAGFHVIGDAAVSAVVGALERVVAGLGVAAVARCGHRLEHLEMVT
                     ADQAAKLGAWGVIASVQPNFDALWGGADGMYAQRLGAERGTQLNPLALLASQGVPLAL
                     GSDAPVTGLDPWVSVRAAVNHQTPGSAVSARAAFAAATRGGWRASGVRDGQTGALVPG
                     APASYAVWDADTLDVHAPRDTVQRWSTDPRSRVPALPRLGPQDALPRCRRTVHRGAVI
                     YG"
     gene            15480..18115
                     /gene="lnt"
                     /locus_tag="VFI55_04430"
                     /pseudo
     CDS             15480..18115
                     /gene="lnt"
                     /locus_tag="VFI55_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290363.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
     gene            complement(18151..18486)
                     /gene="rbpA"
                     /locus_tag="VFI55_04435"
     CDS             complement(18151..18486)
                     /gene="rbpA"
                     /locus_tag="VFI55_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HET7073315.1"
                     /translation="MADRVLRGSRLGAVSYETDRNHDLAPRQIARYRTENGEEFEVPF
                     ADDADIPGTWLCRNGMEGTLIEGNLPEPKKVKPPRTHWDMLLERRSVEELEELLKERL
                     ELIRSRRRG"
     gene            18675..19034
                     /locus_tag="VFI55_04440"
     CDS             18675..19034
                     /locus_tag="VFI55_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073316.1"
                     /translation="MSRRARPQPCRWCGRDVADVGMGRRRQYCRQSCRQRAYEQRASF
                     NRAEPGALLALPSDAVVLSADDAADLSDRVYQVRCAAEDVATALDEGAGASELRELCD
                     VLIRAAQMADGWRRAGV"
     gene            19398..>20291
                     /locus_tag="VFI55_04445"
     CDS             19398..>20291
                     /locus_tag="VFI55_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HET7073317.1"
                     /translation="MGDQIQHATEALRKALVQVERLKRTNRALLERSREPIAIVGMSC
                     RFPGGVDTPEGLWQMVAEGRDVMSEFPADRGWDLAGLFDADPDARHKSYARTGGFVDG
                     VADFDPGFFGIAPSEALAMDPQHRMLLELSWEALERAGIDPSGLRGSATGVFAGLIVQ
                     GYGMLAEEIEGYRLTGMTSSVASGRVSYVLGLEGPAVSVDTACSSSLVALHMAVQSLR
                     SGECDLALAGGATVNATPTIFVEFSRHRGLSPDGRCKSYAGAADGVGWSEGGAVLVVE
                     RLSDARRLGHPVLAVVRGSAVN"
BASE COUNT         3135 a         6754 c         7107 g         3295 t
ORIGIN      
        1 gcggcagcgc ggcgcccagg gcaatccggc cgtctcgctg gctgatccat ccgaccggcg
       61 gccgggtgac cggcgggaac gttgtagcgc tgatgtcggt accgggcagc agactcccga
      121 tgtcggccaa ttcgttgaca cgccaagcat ttccgcgtat ctcgatgaac cgcgcggcaa
      181 gtgcgaccag tgactcggcg atgtcgtcga cagccagccg catgccggtg tcccgccccg
      241 ccaacaacag cgccacaccg tcgtccagca cgtgcacgcc gacatcggcg cacaggccgg
      301 acacgtcggc gcggccgtcg tcgatgctga accagaaccg gccgcccagt tccgcaagcc
      361 ggggatcagc gcagatcgcc gcgtccaact cgctcaccca cggtcgcacg tcggcgcgtc
      421 cgccggtccg gccggacagt ggtgaggcga cgatgttgcg ggcccgctcg tgcgtcggcg
      481 acggcaacag cccgacttcg gcgatcgctt gggcaaccgc tgtgacatcg gtgatgccac
      541 gtaattggac gttgccccgg gcggtcagct cgagccttcc cgagccaagg tcggtcgaga
      601 cccgcgacag ggttgccatc tgttcggcgg tgatggcgcc gccgggtagc cggacccgca
      661 ctagtgcgcc gtcggcggcc cagtgcacct gcagcgcccc ggggcacgcg tcggtatcgc
      721 gggctcttac caccttctaa aggtacggct ccccggcacc gcccgatgcg tgatgagctt
      781 tacacagaga tttctatgaa aatacaaatt ttggctagcg ccgcgggcag tgacggtacc
      841 gtttgctgaa aatccggccc aggaggttcg gctggcggtg caaacggagc gcgttacgaa
      901 ctggccgatg gtgggccgcg acgtcgaact cggtcaggcg ctggcggcgt tcgaggctgg
      961 tccacccctt cggggtgtcg cgctgctggg cgagagcggg gtcggcaagt cgaccctcgc
     1021 gcgaaccctt gccgagacgt tggcttcggg caatcacacc gtgcggttcg tcctgggcac
     1081 cgagaccggc cgtgcaatcc cactgggcgc gttctatcgg tcggtgaccg tcgatgcgac
     1141 gcacgaaccc gccatcatgc tggcgtccgc acaccaggcg ctggaacggg aagaaaacct
     1201 ggtcatcgtc gtcgacgatg cccagttgct cgacccgctg tccgcgacat tggtgcatca
     1261 gctcgccgtc agtggccaaa ccgggctgat cgttgtggtc cgcaccgggg actcagcgcc
     1321 cgacgcggtg acggcgctgt ggaaagaaca gctcctgctg ccgctgagca tcaagccgtt
     1381 cacccgcaag caagtcggcg agctcgcccg gggcgttctc ggcggcgagg tggacggccg
     1441 gctcatcgat gagctgcacc aacgaaccgc cggcaacctg ttgctgctgc gtggcctgct
     1501 cagcgccggc cgggagagcg gggtgctcgc ccggacggag acgggctggc ggctgcatgg
     1561 accgctgcgc ggcgacgacg aactctacga cctggtggag tttcggctgc ggtcgttggc
     1621 gcccgaggaa ctcgaagtca tcgaggtcgt cgcgctcgcc gaagtcctcg actggcacat
     1681 cctgcaggcg gtctgcgacg cctacgcggt cgcgcgcctc gagcgccgag gtttgatcca
     1741 gctcgtcgcc gacggggcgc acgcggtggc gcggttgaac caccccgtca tcggggaagc
     1801 ggcgcttcag cgcgccgggg ttgcgcggac gcggcaactc aacacgctgc tggcgcaaca
     1861 acttcagcag cgttccgcgc acctgcccgc gcccgacgtg cgcacccgga ttcagctggc
     1921 gcagttcatg atccagagcg accttgcccc agacctcgag gtgatcatgg acgccgcggc
     1981 cagcgccgtc accatgtcga acgtcgcctt cggcgagaag ttggcccgct ttgccttcca
     2041 gcgcggcggc ggcctgcggg cggcgatcgt gcttgcggag gcggtgagtt ggcagggacg
     2101 cggcgacgag gccgaagacg tgttgagcgc ctttgacccc gacggttccg acgagctgct
     2161 gacggtgcga tggggctgtc ttcgcgcggc gaacctgttc tggggctgcg gtcgagtcga
     2221 tgcggcgcgc gcggtgctcg ccaacgtgaa agaccgcgtc aattccgggg ccatgctcgg
     2281 tttggcggcg gcgatggggg tgtcgttcgc gttcttctcc ggcgatctgt cgacggcgat
     2341 gacggcggga ctggtcgtgt gtgagtcggg ggtcggaccg cccgcggcgg tgtgggcggc
     2401 gatgtccacc tcgtgggcaa tggcgctgtc cggacgtttc ccggactgtc atcgggtcgc
     2461 caacgcgggt ttccgggccg ccgcgctcgg cgaatccggg ccgcaacggt tcgccatcgg
     2521 gctggccgag gtcatggcgc tgaccgccgc cggggatctg ccggccgccg atttggtctg
     2581 cgaacgctac gcggccgcgg cagcgggtgt gcgcgaggcg gaggcaatcg tgaaagcgct
     2641 ggtgggcctg gccaacttcg cccgcggcgc gctggggccg gcatgtgagg cgttgcggga
     2701 ttcggtagcc gcgatgttga cgggttttcc gtccggctgg ttgatgctgg tttcggcgtt
     2761 actggcccag gccgaagccg gacagcaaca tgacgatgac gcgggttccg cgttgaagct
     2821 ctccgaggac gcgaatggac cgcaagttgc ggtgttcctg ccagaactcg aactggcacg
     2881 cggctgggtg cgggcaagct gcggccagac gagttcggcg caaaggcacg cgttacgcgc
     2941 cgccgagatc gcacgacagt ccgggatgtg tgccgtcgag atgcgtgcgc tacacacggc
     3001 cgtgcggttc ggtgatcgcg cgcaggcggc ccggttgagc gaattggccc ggatcctcga
     3061 cgctgctctg cccgacgtga tcgcggcaca cgcccgcgcc ctggctgacc acgacggcgg
     3121 attgctcgac gacgtcgccg accggttctc gggcatgggc gcgatggcgc tggcggcgga
     3181 tgcggcggct caggccgcac gcgagcacgc ccgcgccggc gagcgggcca aggagctgga
     3241 atcgtcggcg cgggtgcact ggctggcggg caagttcggg ctgcagtcgc cggcgatcga
     3301 tgccgccacc cagccgctgc ccatcaccga ccgcgaacgc gagatcgcgg cgatggtttc
     3361 ggccgggttg tccaatcgtg cgatcgccga ccgactgtcc gtctcggtgc gcacggtgga
     3421 cggacatctt tatcgcatct tcgccaagct cgacatcaaa aaccgcgacc agctcgctcg
     3481 cctactgaac ccggccgagt cccacgcata gaaagttacg gttgtgtgct ccgaggcgct
     3541 caggattgtc cgcgaatacc gaacgtgcga gttcaccact gtgtccggca atggcgcgcc
     3601 gcaaacctgg ccggtgagcc cgttactcct cgaggacggt cgcttcctca ttgcgacgaa
     3661 tatcggcctg ccgcaaaagg cgttgaacgt ccgacgcaat cccaaggtga gcttgctgtt
     3721 ctcggacccg accggatcgg gaatagcgcg gccgggtgcc gttctgattc agggtgacgc
     3781 gatctgcgag gaacgcatcg tgtccgatat cgctcgcgag ccagatcttg ccgcgctcgt
     3841 gcagacggtg ttcggccgcc agcccacggg ctcatcgttc atgatcagcc gacttggccg
     3901 gcggctgttt cccttttact gtctgcgcct cttgattcac gtgctgccgc gccgcgctca
     3961 cttctggcca acacgagact tcgccggccc accggagcag atcgatccca gggacctgca
     4021 acatgtggtc tgatgcggcc agcgagctcg ccggatatcc ggacgcggtg ctcacggctc
     4081 tggaccccgc cggctatccg gtcagcgtgc gccagcgggc gccgcgctac gacccggcca
     4141 cgggacaatg cacggtcagc tggccgtcgg agcttcccgt cgccgcaggc ccggcggtgc
     4201 tgctgtgtca ccgccacgac gagaacctgt ggaatatccg gcagatgcac atcgaaggcc
     4261 gattggagcg ccgtgccgat cagtggatat tcaccagcac cgattttcgg cgtccgccgc
     4321 attcacaact cggtgtcttc tggcggctgg cccgcgatat gcgcagggca ggcagacgct
     4381 acctcgatcg ccggggtctg aaaacaccga cggtgaactg gaaagccctt gaggcactcg
     4441 ccataacacg tcagcagaat tcgggtagtc gcctactcta gctgccgatc cctgccggcc
     4501 ggacactgca aagcagtcca gttacggccg gtcaggggtg caccatggac aagcgcggtg
     4561 gtcatgcagt agtgctcggc gcgagtatgg gcggcttgct ggccgcccgc gtgctggccg
     4621 acttctacgg ccgggtgacg gtggtggagc gggacatctt gccgcgcgat gcggtcaacc
     4681 ggcgaggcgt cccgcaaggg aggttgatcc acgccgccct cgcacagtgc acgggaattc
     4741 tcgacgaact cttccccggg tttgtggagg acctcaaggc cgccggcgtc ggcagctggg
     4801 acgacggcga tctgtccaag ctatggatgt cggtgggctc gcatcaagcg gtgcgttcgg
     4861 gcagggcacc gaatgcgccg cggatcctgt ttccgagccg gcccgtcctc gaatggaatg
     4921 tgcgtcgccg cgtacaggcg atcgacaacg tgatgttcct cgaaggccac gacgtgctcg
     4981 ccctgacggc cacagcgaat cgtggccgcg tcaccggggc gaaggtggtg gatcgcgcca
     5041 acgaccgcga gtcgacgctg accgccgatc tggtcgtcga cgcgaccggc cgcggctccc
     5101 gcacaccgtt ctttctcgag ggactcgggt atgggcgtcc ccgggaggac gagttgatcg
     5161 tccagctcga gtacgcctgt caactgctgc gactcacccc ggacgccgtg cacgagtaca
     5221 tgatcgccct gttccccaag cccggcaggc cgaaaatgtt cgggctcatc aggtacgaaa
     5281 acgacacctg gatgttcggt gtcggcacca tggcgggagc caagccgccg agccggcgtg
     5341 acgacatggt gaccttcgcc gccgatttcg ccccagcgca tgcgatggaa gcgattcgaa
     5401 cggccgagcc ccttggcgag gtggtgcacc atcgcgttcc gtccaaccgc tggcggcgtt
     5461 acgacaagat gcgccgaacg cccgacgggc tgctcgtagt cggcgatgcg gtgtgcagct
     5521 tcaaccccat ctacgggcag ggcatgacgg tcgcggcgat cgaggcgatg gtgctgcgcg
     5581 actgtctgcg gcgcggggat cgcggcctgc cgcgacgctt cttccgcgcc tcggccagga
     5641 aagtccgggt ggcctggcag acggccgtcg gctccgactt gaatctcccg gaagtggtcg
     5701 gaccccgacc gatgtcgatg cgcatcagca actcgttcct cgagcgggtg ctgtccgccg
     5761 tggaggtcga caccgtggtg gcggggcagt tcatgcgggt caccgcaatg gttgatccac
     5821 ccgcgcggct gctgcgtccg tccatcctgc tgcgcctgat gcgcacgagc ggccgtcggg
     5881 cgacggccgc ggggtccgtc gacgagatcg cttcgcgcac agacggcccg cggacgggca
     5941 tcgccatgac gcagaccgga agctaatcgc aagaaacaga agggaatccg atgtccaacc
     6001 agaatatcga gacgacgaag aagggctatg cggctttcgc cgctggcgac ctcgaaaccg
     6061 cgttgagcgt cttcgacgac tctgtcgagt ggaccatcaa cggccagagc acgatcggtg
     6121 gcacgtacca cgggaaaaac gaactcaccg acctgctcat gcggctgtcg gagaagacga
     6181 ccaaggtcga aacaaagcga ttcctggccg acggcgacgt cgtcattgcc ctgaccgaag
     6241 tcaccgccgg cgcagagacc ggtcccgaag ccgacgtgtt cgagttccgc gacggcaagg
     6301 tcgtcagagc cctctcactc ggggacaccg cactgcagga acgcgtcttc ggcagcaagc
     6361 gggtggctgc cgggtagtac ttgcaaagct ggagcgctcg atcaccgcga ggtagccggg
     6421 gcccactggt gaacccccct agggtacgaa tggtgggtgg ctgagccaac catcctgttg
     6481 ctctcgacgt ccgacaccga cctgatcagc gcccgcgcca gcgggaagaa ctatcgctgg
     6541 gccaacccgt cgcggctaac cgatgaccag ctgcctgacc tgctggccaa cgcggcgatc
     6601 gtggtggtcc ggatcctggg cggttacagg gcctggcaga gcggcatcga cgcggtgatc
     6661 gccagcggcg tcccgacggt gctggtcagc ggcgagcagg cggcggacgc ggagctgacc
     6721 ggcctgtcca ccccggcggc cggcatcgcg gtgcaggcgc acatctacct ggcccacggc
     6781 ggggtggaca acctgcgtca actgcacgcc ttcctctcgg acaccgtgtt gatgaccggc
     6841 ttcgggttcg ccccgccggt ggtgacgccg acttggggtg agctggagcg gccgctcgcc
     6901 agccacaccg aaggcccgac catcgcggtg ctgtactacc gggcccagca gctggccggc
     6961 aacaccgcct acatcgaatc gctgtgccgg gccgtcgaag acgccggcgg gcgagcgctg
     7021 cccgtctact gcgcgtcgct gcgcaccgcc gaacccgaac tgttgcaacg gctcggcgag
     7081 gccgacgcca tggtggtcac cgtgcttgcg gcgggcgggc tgcgaccggc caccgtgtcg
     7141 gccggcggcg atgacgacag ctggaacgtc gaacaccttg cggcccttga tattccgatc
     7201 ttgcaagggc tctgcctgac cagtccacgc aggcaatggc tcgacaacga cgacggactc
     7261 agccccctcg acgtggccag tcaggtggcg gtacccgagt tcgacggccg catcatcacg
     7321 gttccgttct cgttcaagga gatcgacgac gacgggctga tcgcctacgt cgccgacccg
     7381 gagcgctgcg ctcgggtcgc gggactggcg atccggcacg cgcaattgcg acatgtcgcc
     7441 ccggccgaca agcgggtggc tctggtgttc tcggcctatc ccaccaagca cgcgcgcatc
     7501 ggcaacgcgg tcgggctgga cacgccggcc agcgcggtcg cgctgctgcg ggcgatgcgc
     7561 gagcgcggct atgacgtggg tgatttgccc ggagtcgacg ccgcagacgg tgacgcgttg
     7621 atccacgcgc tgatcgaacg gggcggacaa gaccccgact ggctgaccga cggccagctg
     7681 gccggcaatc cggtccggat gtccgccaag gattaccgtg actggttcgc gacgctgccc
     7741 gccgaactca ccgacgccgt gcaggcgcat tggggcccac cgccgggagg gctgttcgtc
     7801 gaccggagca acgaccccga cggggagatc gtcatcgccg cgatgcgggc gggcaacgtg
     7861 gtcctgatgg tgcagccgcc gcgtggtttc ggcgagaatc cggtcgcgat ctaccacgac
     7921 ccggacctgc cgcccagcca tcactacctg gccgcctatc actggctgga cgttggattc
     7981 gggtcccacg cattggtgca tctgggcaag cacggcaacc tggaatggct gcccggcaag
     8041 acactgggca tgtcggcggc ctgcggatcc gatgcagcgc tgggcaacct gccgatgatc
     8101 tacccgttcc tggtgaacga tcccggcgag ggaacgcagg ccaagcgacg ggcgcatgcg
     8161 gtcctcgtcg accatctgat cccgccgatg gcgcgcgccg aaacctacgg cgacatcgcc
     8221 cgtttggagc agttgctcga cgagcacgcc gccgtcgccg cgctggaccc cggcaagctg
     8281 ccggccatcc gccagcagat ctggacgctg attcgcgccg ccaaaatgga ccacgatctg
     8341 ggtctgaccg agcggccgga agaggactcg ttcgacgaca tgatcctgca cgtcgacggg
     8401 tggctgtgtg agatcaaaga cgtccagatc cgcgacgggc tgcacatcct gggccaaaag
     8461 ccaacggggg aggccgaact cgacctcgtg ctggccatct tgcgggcccg ccagctgttc
     8521 ggcggcgagc acgtcctccc cggcctgcgg caggcgctgg gcctggccga ggacggcacc
     8581 gacgaacggt cgtcggtcga ccacgccgag gccgcggccc gcgaactggt cgccgccctg
     8641 caggccagcg gctgggatcc cgacggggtc caacggctga ccgacgatgc cgacgtcgcc
     8701 gcggtgctgc ggttcgcggc caccgaggtg gtgccccgcc tggccggcac tgccgccgag
     8761 atcgaccagg tgttaagagc tttggatggc cggttcattc ccgccggtcc gtccggttcg
     8821 ccgctgcgcg gcctggtcaa cgtgctgccc acgggccgca acttctactc cgtcgacccc
     8881 aaggcggtgc cgtcccggct ggcctgggaa gccggtgtgg cgctggccga ttcgctgctg
     8941 gcccggtacc gcgacgacca cgggcagtgg ccgcaatcgg tggggctgtc ggtgtggggc
     9001 acctcggcga tgcgcaccgc cggcgacgac atcgccgagg tccttgcact gctgggtgtt
     9061 cggccggtct gggacgacgc gtcgcggcga gtcgtcggcc tgacgccgat tccgctgacc
     9121 gagctggggc gcccgcgcat cgacgtcacc gtgcggatct cgggcttctt ccgcgacgcg
     9181 ttcccgcacg tcgtgaccat gctcgacgac gcggtgcggc tggtcgccga cctcgacgag
     9241 cccgccgacg acaactacgt gcgcgcgcac gcccaggccg acctggccca gcacggtgac
     9301 caacgtcgat ccaccacaag gattttcggc tccaagcccg gcacctacgg tgccgggctg
     9361 ctgcagctga tcgacagctg caactggcgt gacgacgccg acctcgccgc ggtgtacacc
     9421 gcgtggggag gtttcgccta cggccgtgac ctcgatggcc gcgaggcggt cgacgacatg
     9481 aaccggcaat accgccgtat tgcggtggcc gccaaaaaca ccgacacccg tgaacacgac
     9541 atcgccgact ccgacgacta cttccagtac cacggcggca tggtcgccac ggtgcgcgcc
     9601 ctgaccgggc aagcgccggc ggcgtatatc ggcgacaaca ccaggcccga cgccatccgc
     9661 acccgcaccc tgtccgagga gaccgcccgg gtgttccgcg cccgggtgat caatccgcgc
     9721 tggatggccg cgatgcgccg gcacgggtac aagggcgcgt tcgagatggc ggccaccgtc
     9781 gactacctgt tcggctacga cgccaccgcg ggggtgatgg ccgactggat gtacgagcag
     9841 ctgaccgagc gctacgtgct ggatgccgag aaccggaagt tcatgacgga atccaacccg
     9901 tgggcactgc acggcatggc cgaacggctg ctggaggccg ccgggcgtgg catgtgggcc
     9961 gagccgcaac cggacacact cgacgggctg cgtcaggcgc tgctggaaac cgagggtgac
    10021 ctggagggct agctgcctaa agccggtgag cagacgcaga atcgcataaa aaacaccgtt
    10081 cgcatgcgat tctgcgtctg ctcaccggta ggttgagcag cgtgacgccc accttcgccg
    10141 acctcgccaa ggcgcaatac atcctgctga ccaccttcac caaagacggg cggcccaagc
    10201 ccgttcccat ctgggccgcc gccgacggtg accgactgct ggtcatcacg gaggcaaagt
    10261 cgtggaaggt caagcggatc cgcaacacgc cccgggtgac gatggccacc tgcaccatgc
    10321 gcggccgccc gacgagcgag gccgtcgagg gcacggcggc catcctcgac aagtcgcaaa
    10381 ccggcaccgt ctacgacgcg atcggcaggc gctatggcat cgtgggcacg gtcttcaact
    10441 tcgtcagtaa gttgcgcggc ggcatgcaga acaacgtcgg cctcgagctc agagtggcct
    10501 gaggccaacg cggctcggag accatgatgg gcgcggtgtt ggttccgatc ccggatcgcg
    10561 acttcgaccc gaccgaggtc gcggtgagct ggcgggtcct gacccgcaac ggtcaccgtg
    10621 tggtctttgc caccgaaagc ggcgatcccg gtgtcgccga cgacatcatg gtgaccggcc
    10681 gcgggctgga cgtctggtcc gaactaccgg gcctgagcgc tatcccgctg ctcgggctga
    10741 tgctgcgggc caacaaggac ggccgcgacg cgtatcagga gatgctgcgg tcagccgagt
    10801 accagcgtcc ggtcagctgg gctcaggcca ccctcgatgg cgtcgacgcg ctgctgttgc
    10861 ccggcgggca tcgtgcccgg ggcatgcgca gctacatcga cagcgacgta ctgcatcggc
    10921 ttgtcgtgga cgcctttgcc cgtgggctga tcgtcgccgc gatctgtcac ggtgtgctgc
    10981 tcgccgcgcg cagcgttgat ccgggcacgg gccgctcggt gctctacggc cgcaagacca
    11041 ccgcgttgac gtgggcgatg gagcgcctgg cctggcggct cacccggatc acccgatatt
    11101 gggaccgcaa ttactaccgg acctacgccg aggagcccgg ccagcccagc gggtacatgt
    11161 cggtgcagtc ggaagtcact cgcgcacttg aagatccggc ggacttctgc gacgtggtcc
    11221 gcggatcgcc ggactggttg ctcaaatcct cggggatggt gcgcgacacg ttcaccgact
    11281 cgcgaccggc gttcgtcgtc gacgacggca gctacctatc ggcgcgctgg ccgggcgacg
    11341 tgcacacctt cgccggcgcg ctgtcgcgga aactcccggc cggttagctt atccggggcc
    11401 gatgcagctt ttcagcgctg tggcatgccg ccgtaggtcc gccaccaccg gtagaggctg
    11461 gtgaggtcgg gtcctccgtt gacggtggcg agtagcagct gatcgtcgcg ggtccacgcg
    11521 atgttggcgc ggtccgcgac gatgccgcac acgattttgc cgctgatctg ggcggcgtcc
    11581 gcgcggtaac tccaggtgcc gcgcgacgtg ttttcgccgg ggcaggctga caccgccatg
    11641 ccgtcggccg tcaattgaaa gtcgccggtc agcgcgtcca tgtcgttgta aagcgtgaag
    11701 cgcgcgtagg tcggggtgtc ggagttggtg ttttggctgc agtccaggct ggcgactgcg
    11761 ctcgggaacg ggttcacggc ccgcgtgcag gccgcggcgc tgtacccggg gggcagcagg
    11821 cccacaatct gctgctcggc gccgctgaag cgggcgcggg gaacggtctg ggtcgccgcg
    11881 tcccgcaccg ccccccggct gggctggctg gacggggcgt cgtcccggcc gaacatggcg
    11941 acgcggccca ccacgatcaa caccgccagc acgaccaggg ccacgccgac gtcgacccac
    12001 cgggcatgcc gccggatcgc ccatccgcgc atcgtcgtct ccttactagg cccaccacgg
    12061 gacgatcgcg acgacatatt agcccgcctg acgatcacac cgatggctgc cgtgggcgtt
    12121 tgcccatcgc cgccacagcg cactcggcca acgacgctag gtcgttgcgc ccgtccccag
    12181 gtgctcaccg aagaacgcga agacgcgcct gaacgcgtcc tcggtggcgg cctcgttgta
    12241 cccgaacccc gcgatgcgaa gcagcggttg ggcggggagg gcgttcgcga agctgtgccc
    12301 ggcgtcgggg tacaccttga tgtcggtggc tatctgcttg gccgcggccg tttcgcgcag
    12361 tcgctcgggc gcgcccctgc ccgccagatc acggccgccg aagctcgcca cgatcgggca
    12421 cgccccgtcc agcgtctcgc tgaggttggc gggcagggga gtgccgtaga acggggcagc
    12481 ggcaccaaaa cctttggacg acatgacgag ggcaaaccgg ccgcccatgc agaagcccgc
    12541 gatgccgacc cgcccggagc actccggcat cgcctgcagc tgatcgcggg cggccagaat
    12601 gtcgtcaacg gagcggcccc gctgcatact cacctcccgc atgacccgtg tgatgcatcg
    12661 gacgaggccg ccccgcgaat acagattcgg ggtgagcgcc acatagcccg ccccggcgat
    12721 gcgctcgttg atcgcctcct tgtcgcgccc gtagcccacc gcgtcgtgga tcaccaccac
    12781 acccggcgac ggtcccggcc cctggggaag gctcagcagc gcatcgatcg gtccgtcggg
    12841 gcctcgatct gaattgtggt cataccgtca tctaactgca gccgggatac gcacccgtgg
    12901 gggaactcgg gcgccgcggc tgcacgttgg cgtcacatgt tgcggaggct gttgttgatc
    12961 tacgccattg tcgagctcgc ggcgttcttc ctgctgatct cgacgatcgg gtggggatgg
    13021 gcgttgatgg cgctgttggt cagcttcgtg gtggggtggg gcctgttggc cccgatggca
    13081 ggttcgcacc tgatcggtca aatcggtcga ttgcggtccg ccccggccga accgaatcgc
    13141 gcggtgagcg atggcgcgct ggtcacgctg gccaccactt tggtcctggt tcccggtgtg
    13201 gtcaccacgg cattgggcct gttattgctg attccgccga tacggtcggt cgcccgtccc
    13261 ggattgaccg cgctggggat gcgtgggttg cgccggcgca tgccgctgct caccgacgcg
    13321 acgctgttcg gagccggcgc ccgacgcggg tatgcgggcg atggccgcga ctacatcgac
    13381 ggcgaggtca tcgacgtctt ggacgtcgag ccgccctccc tggcgggcga tcccatgggc
    13441 cacgagttcc cggggcgtcc cgcgtcgtcg acaagctgac gctgcttgcc ctcgtcacca
    13501 ccacacgtag ttttggcgtg tgaccgagac cgcgcaggac cggattccca ccacgttgct
    13561 ggtcaacggc cgggtgcaca gcccgaccca tcccgacgct accgccatgg cggtgcgcgg
    13621 cggcatcgtc gcgtggttgg gcagcgacga cgtcggccgg agccagttcc ccgacgcgga
    13681 cgtggaggat ctggacgggg gcttcgtggc gccgggattc gtcgacagcc atctgcacct
    13741 aacggcgacc ggcctgacgc tcagtgggct ggacttgcgc ccggcggtct cgcgagcgca
    13801 gtgcctccag atggtcgccg actatgcggc cgcccaccct gggcggccgg tgtgggggca
    13861 cggctgggac gagtcgtcgt ggcccgagaa caccccgccg agcagcggcg acctcgatgc
    13921 cgtgctgggt gaccggcccg cctacctggc tcgcgtcgac gtccactccg cactggcctc
    13981 aaccgcgctg cgacgcctgg tcgcagactt accggcggcc gccggtttcg ctccgcagcg
    14041 gccgctggtg ggcgacgcgc accacctgac ccgggccgtc gcccgtgacc tgctgaccgc
    14101 cgagcagctc gccgaggccc gaacggtggc actcgacgcc gccgcggcgg ccgggatcgt
    14161 cgcggtgcac gaatgcgcgg gccccgaaat cggcgggctc gacgactggc tgcggctgcg
    14221 tgacctcaac cacggcgtcg aggtgatcgg ctactggggc gaagcggtag ccacgcccgc
    14281 acaggcgcgc tcgttggtcg acgcgaccgg ggcccatggg ctggccggtg atctgttcgt
    14341 cgacggggcc ctcggatcgc gcaccgcctg gctgcacgag ccgtaccgcg acgcccccga
    14401 ccgcctcggc agctgctacc tggaccacgc cgccatcgag gcccacctgc gggcgtgtac
    14461 cgaggcggag gtgaccgcgg gtttccacgt catcggcgat gcggcggtct cggcggtggt
    14521 gggcgccttg gaacgggtgg tcgcgggcct cggggtggct gcggtcgcgc gctgcggaca
    14581 ccgcctcgag cacctggaga tggtcaccgc ggaccaggcc gccaaactgg gcgcatgggg
    14641 cgtcatcgcg agcgtccagc cgaattttga cgcgctgtgg ggtggcgccg acggaatgta
    14701 cgcccagcgc cttggcgcag aacgaggcac tcaactcaat ccgcttgcgc tgttagcatc
    14761 ccaaggcgtg cccctcgcgc taggctccga cgccccggta acgggcctcg acccgtgggt
    14821 aagcgtgcgg gcagcggtca atcatcaaac cccgggcagt gcggtctcgg cacgggcggc
    14881 gttcgccgct gcgacgcgtg gtggctggcg cgcgagcggt gtccgcgacg gccagacggg
    14941 ggccctggtc cccggcgcgc cggcctccta tgccgtgtgg gacgccgaca cgctggatgt
    15001 ccacgcacct cgcgacacgg tccagcgctg gtcgaccgat ccgcgttcgc gggtgcccgc
    15061 cttgccgcgc ttgggcccgc aggacgccct gccgcggtgc cggcggaccg tgcatcgagg
    15121 cgctgtcatc tatggctaag cactggctcg gccggaaacg gcgcactggc caggaccgcg
    15181 atgtcacccc ggtggccggc gatgcggctg caccaccgga caccgatctg atcagcgacg
    15241 aagccgagtc ggagccagac gaagagccag cggcggttca cgccgaacca aagcaggagc
    15301 cggaactcga gccggagccg gaacccgaac ccgaaccgga gccggaccct gccaccgact
    15361 cgcccgaccc ggaagcgccc gaaaccgcga ccgcggcgca cgttccgagc ctggccgatc
    15421 ggctgggggc cctgacgcgc cgggccctgg cgggtgcggg cgctgcggtg atcgcgcgga
    15481 tgcccgcgat tcgcgccgcg gtgcagccac gcctgccgcg attgctgctc gccgttgcgg
    15541 ccggcgcgct gatgtacacg agcttcccgg cggtgaactg gtggtgggcc gcagtggtcg
    15601 ccgccgcgct gctgggtttg gtgttgaaac accccgccac gacgcctgtg ggtggtttgg
    15661 gctacggatt cctgttcggg ttggcgctct acctgccact gctgccctgg atcagcagcc
    15721 tggtgggcgc cgtgccgtgg ctggcgctgg cggcgacgtg cgcgctgttt cccggcctct
    15781 tcggtctatg cgccgttatc gtgcgcaggc tgcctggctg gccgatctgg ttcgccttgg
    15841 tatgggcggc ccaagagtgg ttgaagtctg tctttccgtt cggcggcttt ccctgggggt
    15901 tggtggcctt cggtcaaacg cagggtccgc tgttgtcgtt ggtccagctc ggcggcgtag
    15961 cgctgctctc gacggcgatc gtgctggtgg gattcagcgc aaccgcgatc gggctggaga
    16021 tcgtgatctg gtggaaaacc ggcaactcgc cgcgcggccg caccgccggc tcgaacgtcg
    16081 tgagaaacac gccggaaacc aggggggccg agccgcccgc cgtggcgctg ccgggtgtct
    16141 cgatttgtct ggtgttattc gcggccgtca tcgtcacgcc gcacgtgcgg cacgccggca
    16201 ccgggtcggg cggcgaaccc acggccaccg tcgccgtcgt gcaaggcaac gttccgcgtc
    16261 tgggcctcga cttcaactcg cagcgccgag cggtgctgga caaccacgtc cgggagacgc
    16321 tgcggttggc cgaggatgtg cgttcggggg ctgccccaca acctgagttc gtgatctggc
    16381 cggaagattc gtcggatatc gaccccctga tcaatgccga tgcggcggaa cagatcacgc
    16441 acgccgtcgc ggcgatcggc gcgccgatac tccttggcac cgttctcgac gtgcccggcc
    16501 gcgccccgga aaatcccgaa tacaccaaca cggtgatcgt gtggaacccg gcgaccggac
    16561 cggccgaccg gcacgacaag gaaatcgtgc agcccttcgg cgagtatctg ccgatgccct
    16621 ggcttttccg gcacctctcc ggatatgcgg accgggccgg ccatatggtg cccggcaaga
    16681 gcagcggggt ggtgcacatc gccggggtgc cggtcggggt ggccacctgc tgggaagtga
    16741 tctttgaccg tgccccgcga aaagccgtcc tgaacggtgc ccagttactg gccgtgccca
    16801 gcaacaacgc cactttcaac aagacaatga gtgagcaaca gctggcgttc gccaaggtgc
    16861 gggcggtaga gcacgacaga tacgtcgtgg ttgccggcac caccggaatc agcgcggtga
    16921 tcgcgccgga cggcgtcgag ttggtgcgca cggacttctt ccagcccgca tatctggaca
    16981 tccaggtgcg cctcaagacg gcgctgacgc cggcgacccg ctgggctccg atcctgcaat
    17041 gggtcctggt cgcgatggcc ggagcggtaa ttctggccgg gatacggcag aatggatggt
    17101 tcgcgcttcc gattcgtctc aggtccaggc cgcataggga atcggaggac gccggtacgc
    17161 ctccggccga aaccgagggc gacctggaaa ccgcgcaaga cgctgcgccc gatgacgagc
    17221 tcggcgagcc gactccgccc ggcaagggcg gtgaagacgg tccgctccac cggggcggcc
    17281 accgactgtt gcgttatctc gggcgacata gaggagctac atgaccaccg gccagcaggc
    17341 gccccgggtc ccggggaatc gtcccagcga gagcgtcttg gtgatcatcc ccacgttcaa
    17401 cgagctggaa aacctgccgg tgatccaccg gcgtctgaaa gaggcgtgcc ccggcgtgca
    17461 cctgctcatc gtcgacgaca gcagccccga cggcaccggc gagctcgccg acgagttggc
    17521 ggcggccgat ccgggtcgta cgcacgtgat gcaccggacg gcgaaggacg gcctgggtgc
    17581 ggcctatctg gccggtttcg cgtggggctt gagccggcag tacgcggtgc tggtcgagat
    17641 ggacgccgac ggcagtcacg cacccgagca gctgcacctg ctgctggacg ctgtcgacgc
    17701 cggagcggac ctggccatcg gttcgcgcta cgtcgagggt ggaacggtgc gaaattggcc
    17761 gtggcgacgc tgggcgctgt cctggacggc caacacctat gcgaggttgg cgcttgacat
    17821 cggtatccac gacatcaccg ccggctaccg cgcttaccgc cgggaagtgc tcgaggcgat
    17881 cgacctcgac ggcgtggatt ccaagggcta ctgcttccag atcgatctga cctggcgcac
    17941 catctgtaac ggtttcgtcg tcgtcgaagt gccgatcacc ttcaccgagc gtgaactcgg
    18001 cgtctccaaa atgagtggat ccaacatccg ggaggcgctg gtgaaggtcg cgaagtgggg
    18061 catcgaaggc cgaaagaccc gtgcgcgcgg cacgggggcc aatacccgca cgtaaccggt
    18121 cgagccgacc ggtccgcgat gagctcgcct ttagccgcgg cggcgtgagc gaatcagctc
    18181 gaggcgctcc ttgagcagct cttcaagctc ttcgacgctg cggcgctcca gcaacatgtc
    18241 ccagtgcgtg cgcggtggct tgaccttctt cggctcgggc aggttgccct cgatgagggt
    18301 gccttccatg ccgttgcggc acagccaggt gccggggatg tcggcgtcat cggcgaacgg
    18361 gacctcgaac tcctcgccgt tctcggtgcg gtaccgggcg atctgacgtg gcgcgagatc
    18421 atggttgcgg tcggtctcgt agctcacggc tccgaggcgg ctgcctctca ggacgcgatc
    18481 agccatgtgt gctactcctt cagggttctc tgggcttcag ttcttgtgca gttcttgtgg
    18541 gccctgtagt gcaattctgt ccgtttagcg aacgctatag cggtcttggg agttcccagg
    18601 ccgacacgct gcatgcgcga cgcgaccatc aatgttaccg gggttgcgca acaccgccga
    18661 ctagagtcgg cggcgtgagt cgccgcgccc gtccccaacc gtgccgctgg tgcggccgag
    18721 acgtcgccga cgtcgggatg ggccgccgtc gccagtactg ccgtcagtcg tgccggcagc
    18781 gggcctacga gcaacgtgct tcgttcaacc gggccgagcc gggggccttg ttggccctgc
    18841 cctccgatgc ggtggtgctg tcggccgacg acgcggccga cctgtcggat cgcgtttatc
    18901 aggtgcgatg cgcggcggaa gacgtggcca ccgcgctgga cgagggcgcc ggggcgagcg
    18961 aactgcgcga actgtgtgac gtgctgatcc gggccgccca gatggccgac ggctggcgcc
    19021 gagcgggtgt ctaacccgcc cccgaccacc accgatgggt ggccgggtac agtcgcgcca
    19081 tggacggcgc gccaggtgta cccagctatg tcggggacca gggaggagcg ggcgcgtcgg
    19141 cgggccgggt cgcgtcgggg tgaccagccg ctcttgccgt cggcgccggg ggaatcggac
    19201 gttttgaaat cctgccggag ttggccatac tcaccagcga cattcccggg tcggcgagca
    19261 gcacgacgct ggccgaaaag cgcctgcggt tcgacaaagc ggcgaggccg cttcgtgcaa
    19321 cttccgtgac tcggctcctg ccgcggcggg cggtcaaagg cggccacatg ttaggtgagg
    19381 gttaggcgag gttagcgatg ggtgatcaaa tccagcacgc gaccgaagcg ctgcgcaaag
    19441 cgctggtcca ggttgaacgc ctgaaacgca ccaaccgtgc gctgctggag cggtcgcgcg
    19501 agccgatcgc gatcgtcggg atgtcgtgcc gcttcccggg cggggtggac accccggagg
    19561 gtctctggca gatggtggcc gagggccgcg atgtgatgtc ggaatttccc gccgatcgcg
    19621 gctgggacct ggccgggctg ttcgacgccg atcccgacgc gcgccacaag tcgtacgcgc
    19681 gcaccggcgg tttcgtggac ggtgtcgcgg acttcgaccc gggtttcttc ggcattgccc
    19741 cgagcgaggc gttggcgatg gatccgcagc atcggatgct gctggaactg tcgtgggaag
    19801 cattggagcg ggccgggatt gatcccagcg ggctgcgcgg cagcgcgacc ggcgtgttcg
    19861 ccggactcat cgtccagggc tacgggatgc tggccgagga gatcgagggc tatcggctga
    19921 ccggcatgac ctccagcgtg gcctcggggc gggtgtcgta tgtgctgggc ctggagggcc
    19981 cggcggtgtc ggtggatacc gcgtgttcgt cgtcgttggt ggcattgcac atggcggtgc
    20041 agtcgctgcg ctcgggcgag tgcgacctgg cgctggccgg cggcgcgacc gtcaacgcca
    20101 cgccgacgat cttcgtggag ttcagccggc accggggctt gtcccccgat ggccgatgca
    20161 agtcgtacgc cggtgcggcc gacggggtcg gctggtccga gggcggggcg gtgctggtcg
    20221 tcgagcggtt gtcggacgcg cggcggttgg gtcatccggt gttggcggtg gtgcggggtt
    20281 cggcggtcaa t
//
LOCUS       DASSGQ010000097         6975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_705959,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000097 DASSGQ010000000
VERSION     DASSGQ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6975
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705959"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..739)
                     /locus_tag="VFI55_04450"
     CDS             complement(155..739)
                     /locus_tag="VFI55_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HET7073318.1"
                     /translation="MSDTGGDMVALRVSDADRNGTMRRLHNAVALGLIDIDEFEQRSS
                     QVSYARTRHELDGLVGDLPGPGAIVTSAADRVELRGWAGSLKRHGEWTVPTRLALVRR
                     LGSIELDLTKARFAGPVVVIELDMRFGSVDIRLPDGASASIDDVEVYVGSASDRRKEA
                     PAEGRPHVVLTGRVLCGSLVVRGPRRSLLRRRAI"
     gene            complement(847..2661)
                     /locus_tag="VFI55_04455"
     CDS             complement(847..2661)
                     /locus_tag="VFI55_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET7073319.1"
                     /translation="MVTRTTLASSVSGLLLVAVIALSGCTPRPDGPAPAAEKFFGALA
                     IGDTATAARLTDDPNEAREALNAAWAGLQATHLDAQILSSKYAEDTGTVNYRFTWHLP
                     KNRTWSYDGQLRMARDEGRWEVRWATTGLHPKLGEHQTFALRADTPRRASVNELGGSD
                     VLAPGYLYHYTLDATQAGPALIDTVHAIVQALQPFNNALTDPQMLAEQASSATQPLDL
                     VTLAPDDNDKVFPVIGRLPGIVITPQADMLPTDPHFAPAVISEVKKAVIDQLNGQAGW
                     RVVSVNQNGVDVAVLHEVEGSPAPSVSITLDRAVQNAAQHAVDTRGGKAMIVVIKPST
                     GEVLAIAQNRGADADGLPATNGLFPPGSTFKMITAGAAIDRDMATPNTMLGCPGHIDI
                     GHRTIPNYGGFDLGVVSMSRAFASSCNTTFAELSSKMPPRGLSQAASRYGIGLDYTMD
                     GITTVTGSVPPTVDLAERTEDGFGQGKVLASPFGMALVAATVAGGKTPVPQLIAGRPT
                     TVEGDNTPISPKMVDALRPMMRLVVTNGTAKEIAGCCGEVYGKTGEAEFPGGSHSWFA
                     GFRGDLAFAALIVGGGSSEYAVRMTKVMFESLPRDYLA"
     gene            complement(2839..3693)
                     /locus_tag="VFI55_04460"
     CDS             complement(2839..3693)
                     /locus_tag="VFI55_04460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7073320.1"
                     /translation="MSAPPIFRLVGERRVSVVRDAAAVWRVLDDDPVGSCMVAARVAD
                     HGIDPNSIGGELWTRRGAEESLCFAGANLIPLRGGLSDLNAFADEAMSGTRRCSSLVG
                     RTDLVMPMWERLAPAWGPARDVRDHQPLMALTSHPSCDIDPGVRQVRPDELDAYLVAA
                     VDMFIGEVGVDPRVGDGGRGYRRRVASLIAAGRAWARFEHGQVIFKAEVGSQSPGVGQ
                     IQGVWVHPEWRGLGLGTGGTAMVAAVIVAGGRVASLYVNNFNTVARAAYARVGFAEVG
                     TFATVLLD"
     gene            complement(3749..4915)
                     /gene="ispG"
                     /locus_tag="VFI55_04465"
                     /gene_synonym="gcpE"
     CDS             complement(3749..4915)
                     /gene="ispG"
                     /locus_tag="VFI55_04465"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HET7073321.1"
                     /translation="MTIGLGMPQPPAPTLAPRRTTRQLMVSNVGVGSDYPISVQSMCT
                     TKTHDVNSTLQQIAELIAAGCDIVRVACPRQEDADALAEIARHSQIPVIADIHFQPKY
                     IFAAIDAGCAAVRVNPGNIKEFDGRVGEVAKAAGAAGIPIRIGVNAGSLDKRFMEKYG
                     KATPEALVESALWEASLFEEHGFGDIKISVKHNDPVVMVAAYEQLAAQCDYPLHLGVT
                     EAGPAFQGTIKSAVAFGALLSRGIGDTIRVSLSAPPVEEVKVGSQILESLNLRPRTLE
                     IVSCPSCGRAQVDVYTLANEVTAGLDGLDVPLRVAVMGCVVNGPGEAREADLGVASGN
                     GKGQIFVRGEVIKTVPEAQIVETLIEEAMRLAAEMQTDHGPGTETSGSPIVTVS"
     gene            complement(4930..6144)
                     /locus_tag="VFI55_04470"
     CDS             complement(4930..6144)
                     /locus_tag="VFI55_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HET7073322.1"
                     /translation="MMFAIGIALFALAILVSVALHECGHMWVARATGMKVRRYFVGFG
                     PTLWSTRRGETEYGVKAIPLGGFCDIAGMTPVEELAPDEADRAMYKQATWKRVATLFA
                     GPGMNFIICLVLIYGIALVWGLPNLHPPSKAVIGETACVAPEVAPGKLGNCTGPGPAA
                     LAGIRPGDVVVKVGDTPVSSFDDMAAAVRKLHGTVPVVVDRDGSAITTYVDVTPTQRY
                     IAKGQGSQPEAATVGAIGVGAVRVTPAHYNALSAIPATFAFAGDLTVEVGKSMVTIPT
                     KVGALVHAIGGGQRDPQTPMSVVGASIIGGDTVDHGLWVAFWFFLAQLNLVLGAINLV
                     PLLPFDGGHIAIAVFEKIRNLIRSGRGMVAAAPVNYLKLMPATYVVLVLVVGYMLLTV
                     TADLVNPIRLFQ"
     gene            complement(6153..>6975)
                     /locus_tag="VFI55_04475"
     CDS             complement(6153..>6975)
                     /locus_tag="VFI55_04475"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HET7073323.1"
                     /translation="VLQAARPGQIVPVDSEHSALAQCLRGGAPDEVAKLVLTASGGPF
                     RGWAAADLEAVTPEQAGAHPTWSMGPMNTLNSASLVNKGLELIETHLLFGIPYDRIEV
                     VVHPQSIVHSMVTFIDGSTIAQASPPDMKLPISLALGWPHRIGGAAPACDFTTACSWE
                     FEPLDSDVFPAVELARHAGQTGGCLTAVYNAANEEAAEAFLQGRIGFPAIVRTIADVL
                     HAADQWAVSPANVEEVLDAQRWARERAQRAIETATATARPAGVSEKVSPMVLERS"
BASE COUNT         1084 a         2382 c         2392 g         1117 t
ORIGIN      
        1 agccgcggag cggcgagggg gagcatcggc ggcgttcagt gtgaacgtgg ggcggaaatt
       61 cgtccgattt cccgctccca gcgcacgctg gacgaggcgc ccggacgaag cggcccgtgc
      121 aacggaccgc cgaggagccg ggcaatcaag tcattcagat ggcccggcgg cgcaacagcg
      181 agcgacgcgg tccgcggacg accagcgacc cgcacagcac ccgccccgtc aacacgacat
      241 gcggccttcc ttccgccggc gcctccttgc ggcggtcgct ggcgctgccc acatagacct
      301 cgacgtcgtc gatcgaggcg ctggcgccgt cgggtaaccg gatgtccacc gagccgaatc
      361 tcatgtcgag ttcgatcacc accaccggcc ccgcgaatcg agccttggtg aggtcgagtt
      421 cgatcgagcc caggcggcgt accagcgcca ggcgggtggg cacggtccat tcgccgtggc
      481 gcttcagcga gccggcccag ccacgcagtt ccacccggtc ggctgcggag gtgacgatgg
      541 cgcccggccc gggcaggtca ccgacgagcc cgtccagctc atgtcgggta cgcgcgtagg
      601 acacctgcga cgagcgctgc tcgaactcgt cgatgtcgat cagcccgagc gccaccgcgt
      661 tgtgcaatcg ccgcatggtg ccgttgcggt cggcgtccga gacgcgcaac gcgaccatgt
      721 ctccacccgt gtcactcatg gactttcctg acgctctgcc aaccggcacc ggaccgggtc
      781 gatttcgcct acactcgcaa cttaccgccg tcggcggtcg gcgggcggcg ctgcaggccg
      841 ctggcgctat gccaggtagt cccgcggcag cgactcgaac atcaccttgg tcatccggac
      901 cgcgtactcc gagctgccgc ccccgacgat cagcgccgcg aaggccaggt cgccgcggaa
      961 ccccgcgaac caggaatgcg atccgcccgg gaactcggcc tcaccggtct tcccgtacac
     1021 ctcaccgcag cagccggcga tctccttggc ggtcccgttg gtcaccacga gccgcatcat
     1081 ggggcgcagc gcgtcgacca tcttcgggct gatcggcgtg ttgtcacctt cgaccgtcgt
     1141 cggccgcccg gcgatcagct gcggcaccgg ggtcttgccg ccggcgacgg tggccgcgac
     1201 caacgccatg ccgaacgggc tggccagcac cttgccctgg ccgaagccgt cttcggtgcg
     1261 ctcggccagg tccaccgtcg gcggcaccga ccccgtcacc gtcgtgatgc cgtccatcgt
     1321 gtagtcgagc ccgatgccat agcggctggc cgcctggctc aggccacggg gcggcatctt
     1381 gctgctcagc tcggcgaacg tggtgttgca ggaactggcg aacgcacgtg acatcgacac
     1441 cacgccgaga tcgaagccgc cgtagttggg gatggtgcga tgcccgatgt cgatatggcc
     1501 cgggcagccc agcatcgtgt tgggcgtggc catgtcgcga tcgatggcgg cgccggcggt
     1561 gatcatcttg aacgtcgatc cgggcgggaa caggccgttg gtggccggta gcccgtcggc
     1621 gtccgcgccc cggttctgcg cgatcgcgag aacctccccg gtcgacggct tgatcacgac
     1681 gatcatcgct ttgccgccgc gggtatcgac cgcgtgctgc gcagcgttct gcaccgcccg
     1741 gtccaacgtg atcgacaccg acggcgccgg tgaaccttcg acttcgtgca gcacggccac
     1801 gtcgacgccg ttctggttga cgctgaccac ccgccagccc gcctggccgt tgagttggtc
     1861 gatcacggcc ttcttcactt cgctgatgac ggccggtgcg aaatgcgggt cggtcggcag
     1921 catgtcggcc tggggcgtga tcacgatgcc gggcagcctg ccgatgacgg gaaagacttt
     1981 gtcgttgtcg tctggcgcca gcgtgaccag gtccagcggc tgggtcgccg agctggcttg
     2041 ttcggcgagc atttgcgggt cggtcaacgc gttattgaaa ggctgtaggg cctgcactat
     2101 cgcgtggacg gtgtcgatca gggcgggccc ggcctgggtg gcgtccagcg tgtagtggta
     2161 caggtagccc ggcgcgagca cgtcgctgcc cccgagttcg ttcaccgaag cgcgccgcgg
     2221 cgtgtcggcc cgcagcgcga acgtctgatg ctcacccagc ttggggtgca gcccggtggt
     2281 ggcccagcgg acctcccagc gcccctcgtc acgggccatc ctgagctggc cgtcgtagct
     2341 ccaggtccgg ttcttgggca gatgccaggt gaagcggtag ttgacggtgc cggtgtcttc
     2401 ggcgtacttc gagctgagta tctgcgcatc caagtgggtg gcctgcagtc cggcccacgc
     2461 cgcgttgagc gcttcgcggg cttcgttggg gtcgtcggtg agtcgggcgg ccgtcgcggt
     2521 gtcaccgatg gccagggcgc cgaagaactt ctcggcggca ggggccgggc cgtcgggccg
     2581 tggagtgcat ccggacagcg cgatgaccgc gacgagcagc aagcctgaga cggatgaggc
     2641 tagtgttgtt ctagttacca tcggtgctga tgttagagct gcggctcgga cacgggcgcc
     2701 gacacaccga gacccaaccg ttatgcacac gcacgtgggc gcgcccccgc gccgaatgtg
     2761 cggccagccg cacgttgggt gccgagcgtg cggccagccg cacgttcgcg cgcgggcggc
     2821 gatcccggag accgccggtc agtccagcag gaccgtcgcg aaggtgccga cctcggcgaa
     2881 gcccactcgg gcgtaggcgg cgcgtgccac cgtgttgaag ttgttgacgt agagactggc
     2941 gacgcgtcca cccgcgacga tcaccgcggc caccattgcg gtaccgccgg tgcccaggcc
     3001 caggccgcgc cactcggggt gcacccaaac tccttggatc tggccgacgc cgggagactg
     3061 cgaccccact tcggctttga agatgacctg accgtgctcg aagcgggccc aggcccgccc
     3121 cgccgcgatc aggctggcca cccgccggcg atagccgcga ccgccgtcgc cgacccgcgg
     3181 gtcgacgccc acttcgccga tgaacatgtc gacggcggcg accaggtagg cgtccagctc
     3241 gtcgggccgc acctgacgca cccccgggtc aatgtcgcag ctcggatggc tggtgagcgc
     3301 catcagcggc tggtggtcgc gcacgtcgcg cgccgggccc caggccggcg cgagccgctc
     3361 ccacatcggc atcaccagat cggtcctgcc gaccagcgac gaacagcgcc gcgtcccgct
     3421 catcgcctcg tccgcaaacg cgttcaggtc ggacagtccg ccacgcagcg ggatgaggtt
     3481 ggcgccggcg aagcacagcg actcctccgc gccgcggcgg gtccacagtt ccccgccgat
     3541 cgaattcggg tcgatgccgt ggtcggcgac ccgggccgcg accatgcacg agccgaccgg
     3601 gtcgtcgtcc agtacccgcc acacggcggc ggcgtcgcgc accactgaca cccgtcgctc
     3661 gccgaccagg cgaaagatgg ggggagccga catcgtggcg aactctctat ggtgcgaact
     3721 gaggatcagt gaggggcgct ggcgcctatc agcttacggt cacaattggc gaaccgctcg
     3781 tttctgttcc cggtccgtga tcggtttgca tctctgcggc cagccgcatg gcttcctcga
     3841 tcagcgtctc gacgatctgg gcttcgggca cggtcttgat cacctcgccc cgaacgaaga
     3901 tctggccctt gccatttccc gacgccacgc ccagatcggc ctcgcgggcc tcgcccggtc
     3961 cgttgacgac gcagcccatc acggcgacgc gtaacggcac atccagccca tcgagccccg
     4021 cggtcacctc gttggccagg gtgtagacgt cgacctgcgc gcgcccgcac gacgggcagg
     4081 acacgatctc cagcgtgcgc ggccgcaggt tcagcgactc gaggatctga cttccgacct
     4141 tgacctcttc caccggcggc gccgacagcg acacccggat ggtgtcgccg atgccgcggg
     4201 acagcagcgc accgaaggcg accgcggact tgatggtgcc ctggaatgcc ggaccggcct
     4261 cggtgacacc gaggtgcagc gggtagtcgc actgtgcggc cagctgctcg taggcggcga
     4321 ccatcacgac ggggtcgttg tgcttgacgc tgatcttgat gtcgccgaag ccgtgctcct
     4381 cgaacagcga ggcctcccac agcgcggact cgacgagggc ctccggcgtg gccttgccgt
     4441 acttctccat gaaccgcttg tccagcgagc cggcgttcac accgatgcgg atcgggatgc
     4501 cggccgcgcc ggcggccttg gcaacctcgc cgacgcggcc gtcgaactcc ttgatgttgc
     4561 ccgggttgac ccgcaccgcg gcgcatccgg catcgatggc ggcgaagatg tacttcggct
     4621 ggaagtggat gtcggcgatc accggaatct ggctgtgccg ggcgatttcg gccagcgcgt
     4681 cggcatcctc ctgacgcggg caggccactc gcacgatgtc acagccggcc gcgatcaact
     4741 cggcgatctg ctgcaacgtc gagttgacgt catgggtctt ggtggtgcac atcgactgca
     4801 ccgagatcgg atagtcgctg cccaccccga cgttgctcac catcagctga cgcgtggtgc
     4861 gccgcggagc aagcgtgggc gccggaggct ggggcatgcc caggccaata gtcactgttc
     4921 cctctcggtt tactggaaga gcctgatcgg attgaccaga tcagcggtca ccgtcagcag
     4981 catgtaaccg accacgagga ccaacaccac gtacgtggcg ggcatgagtt tgaggtagtt
     5041 caccggcgcg gccgcgacca tgccgcggcc cgaccggatg aggttgcgga tcttctcgaa
     5101 caccgcgatc gcgatgtggc cgccgtcgaa gggcagcagc ggcaccaggt tgatcgcgcc
     5161 caggacgaga ttcagctggg cgaggaagaa ccagaacgcc acccacaacc cgtggtcgac
     5221 ggtgtcgccg ccgatgatgc tggctccgac cacgctcatc ggcgtctgcg ggtcacgctg
     5281 cccgccgccg atggcgtgca ccagcgcgcc gacctttgtc gggatcgtga ccatcgactt
     5341 gcccacctcg acggtcaggt ctccggcgaa ggcgaaggtg gccggtatcg ccgacagcgc
     5401 gttgtaatgc gcgggcgtga ccctgacggc accgacgccg atggcgccga cggtggcggc
     5461 ctcgggctga ctgccctgcc ctttcgcgat atagcgctgg gtgggcgtga catcgacgta
     5521 ggtggtgatc gcgctgccgt cacgatccac gacgaccggg acggtgccgt gcaacttgcg
     5581 caccgccgcg gccatgtcat cgaagctgga caccggagtg tcaccgacct tgacgacgac
     5641 gtcaccgggg cggattccgg cgagtgccgc cgggcccggt ccggtgcagt tccccagctt
     5701 gcccggcgcc acttccggag cgacgcaggc ggtttcgccg atgacggcct tgctgggcgg
     5761 gtgcaggttg ggcagccccc acaccagtgc gatcccgtag atcagcacca ggcagatgat
     5821 gaagttcatg ccggggccgg cgaacagtgt cgcgacacgc ttccaggtcg cctgcttgta
     5881 catcgcgcga tcggcttcgt cgggcgccag ctcctcgaca ggggtcatgc cggcgatgtc
     5941 gcagaagccg cccaagggaa tggctttgac gccgtactcg gtctcaccgc gccgggtcga
     6001 ccacagggtg gggccgaagc cgacgaaata gcgacgcacc ttcatcccgg tggcgcgggc
     6061 gacccacatg tggccgcatt cgtgcagggc caccgagacc aggatggcga gcgcgaacaa
     6121 cgcaatgccg atagcgaaca tcatcttcgc tgctaggacc tttctaagac cattggggag
     6181 accttctcag agactcccgc ggggcgcgcg gttgcggttg ccgtctcgat agcccgctgc
     6241 gcccgctccc gtgcccaacg ctgcgcatcc agtacctctt ccacgttagc gggtgaaacg
     6301 gcccattggt cggcggcgtg cagcacgtcg gcgatggttc tgacgatggc ggggaagccg
     6361 atccggcctt gaaggaatgc ctctgccgcc tcttcgttgg cggcgttgta gacggcggtc
     6421 aggcagccgc cggtctggcc ggcatgccgg gccagctcga ccgcggggaa gacatcgctg
     6481 tccagcggct cgaattccca gctgcaggcg gtagtgaagt cgcaggcagg agcggcgccg
     6541 ccgatccggt gcggccaccc cagggccagc gaaatgggca gcttcatgtc cggggggctg
     6601 gcttgggcga tggtcgaacc gtcgatgaac gtgaccattg agtggacgat cgactggggg
     6661 tgtacgacga cctcgatgcg gtcgtagggg atgccgaaca acaggtgggt ttcgatgagc
     6721 tccagcccct tgttgaccag cgacgccgag ttcagcgtgt tcatcgggcc catcgaccag
     6781 gtggggtggg cgccggcctg ctcgggcgtg acggcctcga ggtcggcggc ggcccagccg
     6841 cggaacggac cgcccgaggc ggtcagcacg agcttggcga cctcgtcggg ggcgccaccg
     6901 cgcaggcact gggccagcgc ggagtgttcg gagtcgacgg gcacgatctg gcccggccgc
     6961 gccgcctgca gcacg
//
LOCUS       DASSGQ010000098         9865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_158921,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000098 DASSGQ010000000
VERSION     DASSGQ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9865
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158921"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1415
                     /gene="ftsZ"
                     /locus_tag="VFI55_04480"
     CDS             261..1415
                     /gene="ftsZ"
                     /locus_tag="VFI55_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET7073324.1"
                     /translation="MTPPHNYLAVIKVVGIGGGGVNAVNRMIEQGLKGVEFIAINTDA
                     QALLMSDADVKLDVGRDSTRGLGAGADPEVGRKAAEDAKDEIEELLRGADMVFVTAGE
                     GGGTGTGGAPVVATIARKLGALTVGVVTRPFSFEGKRRGNQAETGIAALRESCDTLIV
                     IPNDRLLQMGDAAVSLMDAFRSADEVLLNGVQGITDLITTPGLINVDFADVKGIMSGA
                     GTALMGIGSARGEGRSLKAAEIAINSPLLEASMEGAQGVLMSIAGGSDLGLFEINEAA
                     SLVQDAAHQDANIIFGTVIDDSLGDEVRVTVIAAGFDAQGPGRKPVVGAGAPEKPGGA
                     HRIETARAGKLTSTLFEPVDAVSVPVHTNGSTLNIGGDDDDVDVPPFMRR"
     gene            1507..2223
                     /gene="pgeF"
                     /locus_tag="VFI55_04485"
     CDS             1507..2223
                     /gene="pgeF"
                     /locus_tag="VFI55_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HET7073325.1"
                     /translation="MSVRIRRVTTTRAGGVSKAPFGTFNLGDHVGDDPAAVAANRARL
                     ASALGLGADRVVWMNQVHGDRVEVVDGPQSAAVGGGVGTDALVTRTPRLALAVVTADC
                     VPVLIADARAGVIAAVHAGRVGAQRGVVARAVEAMLGLGARAGDISVLLGPAVSGRNY
                     EVPAVMADEVEAALPGSRTTTAAGTPGLDLRAGIACQLKDLGVKAIDVDPRCTVADST
                     LFSHRRDAPTGRLASLVWME"
     gene            2228..3013
                     /locus_tag="VFI55_04490"
     CDS             2228..3013
                     /locus_tag="VFI55_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET7073326.1"
                     /translation="MAADRLARENHGNRESELTLALAGVRSRLAAAAEAAGRNVGEIE
                     VLPITKFFPATDVAILSRLGCRSVGESRDQEASAKVAEVARLASAPPRAALRDMHWHM
                     VGRIQRNKARSLANWAHTAHSVDSPRLAIALDRAVSAALGDGRRQYPLRVYVEVSLDG
                     DVSRGGVDVLTRGAVDRVCAQVEESKSLELVGLMGIPPLGWEPGEAFDRLQAEHRRVL
                     ETYPNAVGLSAGMSSDFEIAVKHGSTCVRVGTALMGPRRLPSP"
     gene            3081..3737
                     /locus_tag="VFI55_04495"
     CDS             3081..3737
                     /locus_tag="VFI55_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HET7073327.1"
                     /translation="MSTLHKVKAYFGMAPMEDYDDEYYDDRAPSRGFPRPRFDDGYGR
                     MEGRYEGRDYDDPRGEPADYPPSSGYRGGYGEESRYSPVHPREFDRSELGRNRVGSWL
                     RSSTRGALAMDPRRMAMLFEEGHPLSKITTLRPKDYSEARTIGERFRDGTPVIMDLVS
                     MDNADAKRLVDFAAGLAFALRGSFDKVATKVFLLSPADVDVSPEERRRIAETGFYAHQ
                     "
     gene            3892..4182
                     /locus_tag="VFI55_04500"
     CDS             3892..4182
                     /locus_tag="VFI55_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET7073328.1"
                     /translation="MVLFFQILGFALFIFWLLLIARVVVEFIRSFSRDWRPTGITVVI
                     LEIIMSITDPPVKLLRRLIPQLTIGAVRFDLSIMVLLLVAFIGMQLAFGAAA"
     gene            4436..5239
                     /locus_tag="VFI55_04505"
     CDS             4436..5239
                     /locus_tag="VFI55_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HET7073329.1"
                     /translation="MPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIE
                     ENSDLRQRIEELDRELAAGGGGGGGATQVTQAIPTFEPEPEPVKPAPVAAAAPVVSGS
                     NEEHALKAARVLTLAQDTADRLTSTAKAESDKMLADARANADQILSEARHTAETTVTD
                     ARQRADAMLTDAQSRSETQLRQAQEKADALQADAERKHSEIMGTINQQRTVLEGRLEQ
                     LRTFEREYRTRLKTYLESQLEELGQRGSAAPVDSSADAGGFDQFNRGSN"
     gene            5335..5661
                     /locus_tag="VFI55_04510"
     CDS             5335..5661
                     /locus_tag="VFI55_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073330.1"
                     /translation="MLIIALVLALIGLVALVFAVVTSNELVAWVCIGASALGVVLLII
                     DALRERQRRGAGDEDHDEDAAVDYPEEASDKAEPTADAEAGESSEEAATDASEGSTVS
                     GDDRAK"
     gene            complement(5721..6398)
                     /locus_tag="VFI55_04515"
     CDS             complement(5721..6398)
                     /locus_tag="VFI55_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HET7073331.1"
                     /translation="MNPPRGFLRRTATRTFRDRREAGRVLAEELSCYRDKDGVLVLGL
                     ARGGVPVAWEVAASLRAPLDVFLVRKLGVPRWPELAMGALASGGAVVMNDNVVSSLQI
                     SDDQVREVIASEKAELIRREQAYRRGRPIADTRGKIAILVDDGIATGASMLVAVRALR
                     AAGPRSIVVAVPVGPQSTCHDLAREADDVVCATMPPGFEAVGQVYADFHQVSDNEVGK
                     VLDTPTC"
     gene            6651..6857
                     /locus_tag="VFI55_04520"
     CDS             6651..6857
                     /locus_tag="VFI55_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073332.1"
                     /translation="MSRRFNTYTSYSVACAIVWAVILVGVSLAADSATRRTFLLICAG
                     WAIGWLSATIARFVYPPPKRQLGA"
     gene            complement(6864..6949)
                     /locus_tag="VFI55_04525"
     tRNA            complement(6864..6949)
                     /locus_tag="VFI55_04525"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6914..6916),aa:Leu,seq:gag)
     gene            6968..7141
                     /locus_tag="VFI55_04530"
     CDS             6968..7141
                     /locus_tag="VFI55_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073333.1"
                     /translation="MAAKAIGWARTDPTQLADDAAREAGRGEGGQIQPTSPTMRAREV
                     KAAGARAERPRQW"
     gene            7153..8508
                     /locus_tag="VFI55_04535"
     CDS             7153..8508
                     /locus_tag="VFI55_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7073334.1"
                     /translation="MTVETGAREVPTHPSDDVVEVVSRLIRFDTTNTGEPETTRGEAE
                     CARWVAEQLREVGYLPQYVESGAPGRGNVFARLKGADTSRGALLIHGHLDVVPAEQTD
                     WSVHPFSGAIKDGYVWGRGAVDMKDMVGMMIVVARQLKRAGVVPPRDLVFAFLADEEH
                     GGHYGAQWLVDNRPELFDGITEAIGEVGGFSLTVPRRDGGERRLYLIETAEKGLSWMR
                     LTARGTAGHGSMVHDHNAVTAVAEAVARLGRHRFPLVLTDTVAQFLAAVSEETGLAFD
                     GPSTDLEEMIEKLGPTARMLKAVLHDTANPTMLKAGYKANVIPATAEAVVDCRILPGR
                     KAAFEAEVDELIGPDVTREWIRDFSSYETSFDGDLVDAMNAALLALDPDGRTVPYMLS
                     GGTDAKAFARLGIRCFGFSPLRLPPDLDFSALFHGVDERVPIEALHFGIDVLAHFLTH
                     C"
     gene            8562..9092
                     /locus_tag="VFI55_04540"
     CDS             8562..9092
                     /locus_tag="VFI55_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET7073335.1"
                     /translation="MSLPPDPYDTLPKLPLFSLTSNSITDGQPLATPQISGILGAGGE
                     DVSPQLSWSGFPKETRSFAVTVYDPDAPTLSGFWHWAVANLPADVTELPEDAGDGREL
                     PGGAVSLVNDAGMRRYVGSAPPPSHGVHRYYFAVHAVDVDKLELTEDASPAYLGFNLF
                     QHAIARAYIVGTYEQT"
     gene            9164..9730
                     /locus_tag="VFI55_04545"
     CDS             9164..9730
                     /locus_tag="VFI55_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010595508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7073336.1"
                     /translation="MLVLPGGKVRSEDASRWWQPANLRMMLIAAALRRRLGPDVEVRR
                     VQYRCRGWNSPRLDAVCDARAVLDEVRRRFEPKRIVLVGHSMGGRVAAQLCAGAGVGG
                     VVALAPWWVGGDADLIPESTRLLVVHGTADTWTDPRSSRIQTRRAGEHGLDAQWVGVA
                     GARHYMLRRCGEWHRRTTDFVADYLSCS"
BASE COUNT         1649 a         3158 c         3406 g         1652 t
ORIGIN      
        1 gtagtgccgc cacaccggct gttggtggct gttggtggct attggtgccg attccaggcc
       61 aagccgggcg cgacagctcg tcgcgcgcgc gacaaagaaa aaattgcgcg ccgcgtgtcg
      121 gcgcgcctgc agggctagcg cgcgtcctac ccataccgtt ctgtttgcgc ggaactactt
      181 gacataactc taagcctatg gttgaggttg agggtttgca agccgacaca ccgagtccca
      241 ccagggagga ataacgaatg atgacccccc cgcacaacta cttggccgtg atcaaggttg
      301 tgggtatcgg tggcggcggc gtcaacgccg tcaaccggat gatcgagcaa ggcctcaagg
      361 gcgtggaatt cattgcgatc aacaccgacg cccaggcgct gttgatgagc gatgccgacg
      421 tgaagctgga cgtcggacgc gactccacgc gcgggctggg cgccggcgcg gaccccgagg
      481 tggggcgtaa ggccgccgag gacgccaagg acgagatcga ggaactgctg cggggggcgg
      541 acatggtgtt cgtcaccgcc ggcgaaggcg gcgggaccgg caccggcggg gcgcccgtcg
      601 tcgccaccat cgcccgcaag ctgggcgcgc tgaccgtcgg cgtggtcacc cggccgttct
      661 cgttcgaggg caagcggcgc ggtaaccagg ccgaaactgg cattgctgcg ctccgggaga
      721 gctgcgacac ccttatcgtg atccccaacg accggctgct gcagatgggt gatgccgcgg
      781 tatcgctgat ggatgccttc cgcagcgccg acgaggtgct gctcaacggc gtgcagggca
      841 tcaccgacct gatcaccacc ccgggtctga tcaacgtcga cttcgccgac gtcaagggca
      901 tcatgtccgg ggccggcacc gcgctgatgg gcatcggctc ggcccgcggt gaaggccgtt
      961 ccctcaaggc cgccgagatc gccatcaact caccactgct ggaagcgtcg atggagggcg
     1021 cccagggcgt gctgatgtcg atcgccggcg gcagcgacct gggcctgttc gagatcaacg
     1081 aggcggcatc gctggtgcag gacgccgccc atcaagacgc caacatcatt ttcggcaccg
     1141 tgatcgacga ctcgctgggc gacgaggtcc gcgtcaccgt catcgcggcg ggcttcgacg
     1201 cccaaggccc cggccgcaaa cccgtggtcg gtgccggtgc tccggagaag cccggcggcg
     1261 cgcacagaat cgagaccgcg agagccggca agctcacctc gacgttgttc gagcccgtcg
     1321 atgccgtcag cgtgccggtt cacaccaacg gctcgaccct gaacatcggc ggcgatgacg
     1381 acgatgtcga cgtgccaccc ttcatgcgcc gctgagggtc tcaatttgct tgccagaacg
     1441 cgacaaactg gccgcggccg cacgttggcg ccccctggtc ggcagcaggt tccggatact
     1501 aggcgtgtga gcgtccgtat ccgtcgggtg accaccaccc gagcgggcgg tgtgtcgaaa
     1561 gcgccgttcg gcaccttcaa cctcggcgac catgtcggtg atgatccggc ggcggtggcg
     1621 gccaaccggg cccggctggc ctccgcgctt ggcctcggcg ccgaccgggt ggtgtggatg
     1681 aaccaggtcc acggagatcg agtcgaggtg gtcgacgggc cgcagagtgc cgccgtcggc
     1741 ggcggggtgg gcaccgacgc gttggtgacc cgcacccccc ggctggcgct ggcggtggtg
     1801 acggccgact gcgtgccggt gttgattgcc gatgcgcgcg ccggtgtgat cgcggcggtg
     1861 cacgcgggcc gcgtcggtgc gcagcggggg gtggtggcgc gcgcggtgga ggcgatgctg
     1921 gggctgggcg cgcgggccgg cgacatctcg gttctgctcg gcccggcggt gagcggccgc
     1981 aactacgaag tccccgcggt gatggccgac gaggtggagg cggccctgcc gggcagccgc
     2041 accaccactg cggccggcac tccagggctc gaccttcggg ccggaatcgc ttgccagctc
     2101 aaggatttgg gtgtcaaggc aatcgacgtg gatccgcgct gcacggtggc cgactcgacg
     2161 ctgttcagtc atcgccggga tgcgccgacg gggcggctgg cctcgctggt ctggatggag
     2221 tgaccgcatg gcggcggaca ggctggcccg cgaaaaccat ggcaaccgcg aatcggaatt
     2281 gacacttgcg ttagccggcg tgcgctcgcg acttgcggcg gccgcggaag cggcgggacg
     2341 caatgtcggc gaaattgaag tgctgccgat taccaaattc tttccggcaa cagatgtggc
     2401 gattttgtct cgattgggtt gccggtccgt cggcgaatct cgggatcagg aagcttcggc
     2461 gaaggtggcc gaagttgccc ggttggcttc ggctcccccg cgggctgccc tgcgggatat
     2521 gcactggcac atggtgggcc ggatccagcg gaataaggcg cgctcgcttg ccaattgggc
     2581 gcacaccgcg cattcggtcg acagcccgcg gctggcgatt gcactcgatc gcgcggtatc
     2641 ggcggcgctg ggcgacggcc gccggcagta cccgttgcgg gtatacgttg aggtcagcct
     2701 cgacggggat gtgtctcgcg gaggggtgga cgtgctcacg cggggtgcgg tcgatcgggt
     2761 ctgcgcgcag gtcgaggagt cgaaaagcct ggaattggtg gggttgatgg gcatcccgcc
     2821 gctgggctgg gagcccggcg aggccttcga ccggctgcaa gccgagcatc gccgggtgct
     2881 ggaaacgtat ccgaatgcgg tcgggctgtc cgccggcatg tccagcgatt tcgagatcgc
     2941 cgtcaaacat ggttcgacat gtgtgcgtgt cggtaccgcg ctaatgggtc cgcggcggct
     3001 accgtcaccg tgaatagtca ctgtagtcac attttcatca cagacatcac aactcccagg
     3061 ggctagaagg ggttcacgca atgagcacac tgcacaaggt caaggcctac ttcggcatgg
     3121 ctccgatgga ggactacgac gacgagtact acgacgaccg cgccccctcc cgcgggttcc
     3181 cgcgtccgcg gttcgacgac ggttacggtc gcatggaggg ccgctacgag gggcgcgact
     3241 atgacgaccc gcgcggcgag ccggccgatt acccgccgtc cagcggttat cgcggcggct
     3301 acggcgagga gtcccgttac agtccggttc acccccgcga gttcgatcgg tccgagctgg
     3361 gccgcaaccg ggtggggtcg tggttgcgga gttccacccg cggagcgctg gccatggatc
     3421 cgcgccggat ggcgatgctg ttcgaagaag gccacccgct gtcgaagatc accacgctgc
     3481 ggcccaagga ctacagcgag gcgcgcacca tcggcgagcg gttccgggac ggcacgccgg
     3541 tcatcatgga cctggtgtcg atggacaacg ccgacgcaaa acggctggtc gacttcgcgg
     3601 ccggcttggc cttcgcgctg cgcggctcgt tcgacaaggt cgctaccaag gtgttcctgc
     3661 tgtcgccggc cgacgtcgac gtgtccccgg aggagcgtcg ccgcatcgcc gaaaccgggt
     3721 tctacgctca ccaatagccg cacctggtcg ggcccggcgc ccgtcctggc gcgacgcggt
     3781 ccacgtgaga tgtgtgcgcc catcatggag tcggtacgct ggcgactacc gcattagcgc
     3841 gcggcgacta cgtcgtcggt catctcatcg gttaggtcgg ggctactaga gttggtgctg
     3901 ttctttcaga tccttgggtt tgcgctgttc atcttctggc tgctgctcat cgctcgggtc
     3961 gtcgtcgagt tcatccgctc gttcagccgt gactggcggc ccaccggcat caccgtggtg
     4021 atcctcgaga tcatcatgtc gatcaccgat ccgccggtga aactgctgcg ccggctcatc
     4081 ccgcaactca ccattggagc ggtccgtttc gacctgtcga tcatggtgct gctgctggtc
     4141 gcgttcatcg gcatgcaact ggcgttcggc gccgcggcgt gagccaggcg ctggcccccg
     4201 cgacagaagt ccggcgacag ttcggctact tttccgcgtc ggtttggtcc ccgcgatttg
     4261 aaattgttaa ggattcggat tttattgctc ttaatgtttg aaatcggttc ttaattatcg
     4321 ggctaatggg ctaccgccgg gtctggtgtg acaggatgga cgccagttgc gcgacggcaa
     4381 ctactccgtt ctacactttc cagatcgttt gacaggaact tgaggggaac gaacaatgcc
     4441 gcttacaccc gccgacgtcc acaatgtggc gttcagtaag ccgccgattg gcaagcgcgg
     4501 ttacaacgaa gacgaggttg acgccttcct cgacctagtg gagaacgagc tgacgcggct
     4561 gatcgaagag aactccgatc tgcgtcaacg gatcgaggag ctggaccgcg agctggccgc
     4621 cggcggcggc ggtggtggcg gtgcgactca ggtcacccag gcgattccca ccttcgagcc
     4681 cgaaccggag ccggtcaagc cggcgccggt cgcagccgcg gcgcccgtgg tgtcgggaag
     4741 caacgaggaa cacgccctga aggccgctcg ggttctcacc ctggctcagg acaccgcgga
     4801 ccggttgacc agcaccgcaa aggccgaatc ggacaaaatg ctggcagatg cccgcgccaa
     4861 cgccgaccaa atccttagcg aggcacggca caccgccgag accacggtca ccgacgcccg
     4921 tcaacgcgcc gacgcgatgc tgaccgacgc ccaatcgcgt tccgagaccc agctgcgcca
     4981 ggctcaggag aaggccgatg ccctgcaggc cgacgcggag cgcaagcatt ccgagatcat
     5041 gggaaccatc aaccagcagc gcacggtgct ggaaggccgg ctcgagcagc tacgcacctt
     5101 cgaacgcgag taccgcaccc ggctcaagac ctacctggaa tctcagctcg aggaactcgg
     5161 ccagcgcgga tcggcggcac ccgtagactc cagcgccgac gcgggtgggt tcgatcaatt
     5221 caatcggggt agtaactagc cggggaccgt gtccgacact cgctaagctt cgaggtcggc
     5281 acacaacgtg cactagacct ggcagccccg tttcggcggg tggaggaatg accgatgctg
     5341 atcattgcgc tggtactggc cctcatcggg ctcgtggcgt tggtgttcgc cgtcgtcaca
     5401 agtaacgagc tggtcgcatg ggtatgcatt ggcgccagcg ccttgggtgt ggtgctgctg
     5461 atcatcgacg cgttgcggga gcgccagcgg cggggcgccg gcgacgaaga ccatgacgaa
     5521 gacgcggccg tcgactatcc cgaggaagcg tcggacaagg ccgagccgac ggcggacgcc
     5581 gaggccggtg agtcgtcgga ggaggccgcc accgacgcca gcgaggggtc gacggtatcc
     5641 ggcgacgacc gggccaagta gtccagccca atagcgctct tcaacaccgt tcagccgctc
     5701 tccaagaggc ctggactggc tcagcaggtc ggcgtatcca gcactttgcc aacctcgttg
     5761 tcgctgacct ggtggaagtc ggcgtagacc tgcccgacgg cctcgaaccc gggcggcatg
     5821 gtcgcgcaca ccacgtcgtc ggcttcccgc gcgaggtcgt ggcaggtgga ctgcggcccg
     5881 accggcaccg ccacgacgat cgaccgcgga cccgcggccc gcagggctcg gacggcgacc
     5941 agcatgctgg cgccggtggc gatgccgtcg tcgaccagga tcgcgatctt gccgcgcgta
     6001 tcggcgatcg ggcgcccgcg ccgatacgcc tgttcccgcc ggatgagttc ggctttctcg
     6061 ctggcgatca cttcgcggac ctggtcgtcg ctgatctgca agctggacac cacgttgtcg
     6121 ttcatcacaa ccgcgccgcc gctggccaac gcgcccatcg caagctccgg ccagcgcggt
     6181 accccgagct tgcggaccag gaagacatcc agcggggcgc gcagtgacgc ggcgacttcc
     6241 caggccacgg gaacgccacc gcgcgccaac cccaaaacca gcaccccgtc cttgtcgcga
     6301 tagcacgaca actcctcggc cagcacgcga cccgcttcac gacggtcgcg gaacgtgcgc
     6361 gtcgccgtgc gccggaggaa gccgcggggt gggttcattg gtcacactcc aggctaccgc
     6421 tttggccacg ccgcagagag ccgaacaaaa tctgcccaac cccgctgaat cggtcttact
     6481 atgggttaca tcacgttgtg cgggaacgga attggcggcg ccgatgctgt tggtggctcg
     6541 gcgccttggc cggttgtgat gaaaactcgt gctcggacac gagccgctgc gcgtttgcga
     6601 ccggatctcg ccttgcaggt cgagccacga cacgatcgaa aggatccgcg atgagtcgcc
     6661 gcttcaacac ttacacgtcc tacagcgtgg cctgcgcaat cgtgtgggcc gtcatcctgg
     6721 tcggagtttc gttggcggcc gatagcgcca cccggcgcac gttcttgctg atttgcgctg
     6781 ggtgggcgat cgggtggctg tcggcgacga tcgccaggtt cgtctacccg ccgccaaaac
     6841 ggcagcttgg agcataaaac gggtgtccga ggggggactt gaacccccac gcccattagt
     6901 agggcactag cacctcaagc tagcgcgtct gccattccgc cactcggaca acaccaaacc
     6961 acatgggatg gcagctaagg ctatcggatg ggcccgcacg gacccaaccc agctcgccga
     7021 cgatgcggcc cgcgaggcgg gccgaggaga aggcgggcaa atccaaccca cctcgccgac
     7081 gatgcgggcc cgggaggtca aggcggccgg agcccgcgcc gagcggcccc ggcagtggta
     7141 ggaaaggggg ttgtgaccgt tgagaccggg gcgagggagg ttccgaccca cccgagcgat
     7201 gatgtggtcg aggttgtcag caggttgatc cggttcgata ccaccaacac cggcgaaccc
     7261 gagacgacgc gcggcgaggc cgagtgcgcg cggtgggtcg ccgagcagct ccgagaggtc
     7321 ggctacctgc cgcagtacgt cgaatccggc gcacccggcc ggggcaacgt gttcgcgcgc
     7381 ctcaagggcg ccgacacctc tcgcggcgcc ttgctcatcc acgggcatct cgacgtggtg
     7441 ccggccgagc agaccgattg gagcgttcac ccgttttccg gtgcgatcaa ggacggctac
     7501 gtgtggggcc gcggtgcggt cgacatgaaa gacatggtcg gcatgatgat cgtggtggcg
     7561 cggcagttga agcgggccgg cgtcgtgccg cctcgcgatt tggtgttcgc gttccttgct
     7621 gacgaagagc acggcggtca ttacggggcg cagtggttgg tcgacaatcg gcccgagctg
     7681 ttcgacggga tcaccgaggc catcggggaa gtcggcgggt tctcgctgac cgtgccacgc
     7741 cgcgacggcg gtgaacgtcg gctctatctg atcgagacgg ccgagaaggg gctgtcgtgg
     7801 atgaggctga ccgcccgggg cacggccgga cacggctcga tggtgcacga ccacaacgcg
     7861 gtcaccgccg tcgccgaggc tgttgcccgg ttgggtcggc accggtttcc gctggtgctg
     7921 accgataccg tcgcccagtt tctggccgcc gtgagcgagg agaccgggct ggctttcgac
     7981 ggcccgtcga ccgatctcga ggaaatgata gaaaagctcg gcccgacggc ccggatgctc
     8041 aaggccgtgc tgcacgacac cgccaacccg acgatgctca aggccgggta caaggccaac
     8101 gtcatcccgg cgacggccga agccgtggtg gactgccgca tcctcccggg ccgcaaggcg
     8161 gcgttcgagg ccgaggtcga cgagctgatc ggccccgacg tgacgcgcga atggatcagg
     8221 gacttttcgt cctacgagac cagcttcgac ggtgacctgg tcgacgcgat gaacgccgcg
     8281 ctgctcgcgc tggacccgga cggccggacg gtgccctaca tgctttccgg ggggaccgac
     8341 gctaaagcct tcgcgcgctt gggtattcgc tgtttcggtt tcagcccgct gcggttaccg
     8401 ccggatttgg atttcagcgc attgttccac ggcgtcgacg agcgggtacc catcgaagcg
     8461 ctgcatttcg gcatcgatgt gctggcacac ttcctgacac actgctagtc caagctagtg
     8521 cgaatttatt gagcccctaa acacatcacg agaggatcgc catgagcttg ccgcccgacc
     8581 cgtacgacac gctgcccaag ctgccgttgt tcagcctgac ctcgaactcg atcaccgacg
     8641 gccagccgct ggcgacaccg cagatcagcg ggatcctagg tgccggcgga gaggacgtga
     8701 gcccgcagct gagctggtcg ggattcccga aggagacccg cagtttcgcg gtcacggtct
     8761 acgaccccga cgccccgacg ctgtccgggt tctggcactg ggcggttgcc aacctgcccg
     8821 ccgacgtgac cgagttgccc gaggacgcgg gcgacggccg tgagctgccg ggcggcgcgg
     8881 tgagcttggt caacgacgcc gggatgcgcc gctatgtcgg ctccgcgccg cctcccagtc
     8941 acggggtgca tcgttactac tttgcggtgc atgccgtgga cgtcgacaaa ctcgagctca
     9001 ccgaggacgc cagccccgcc tatctgggct tcaacctgtt ccagcacgcg atcgcgcgcg
     9061 cgtacatcgt cggcacctac gaacagacgt agggcggcca gcgcgacttt tccaaccgcg
     9121 tcggcgccgg ctccgaatca ccgtcgtgga accgcgcgcc tgtgtgttag tcctgccggg
     9181 cggcaaggtg cgcagcgagg acgcctcccg ctggtggcaa ccggccaacc tgcggatgat
     9241 gttgatcgcc gccgcgttgc gacgccggct gggtcccgat gtcgaggtgc ggcgcgttca
     9301 gtaccgctgt cgcggatgga atagcccgcg gttggacgcc gtgtgcgacg ccagggcggt
     9361 gctcgacgag gttcggcggc gattcgaacc gaaacgcatt gtgctggtgg ggcattcgat
     9421 gggcgggcgg gttgcggcgc agctgtgtgc gggcgcgggc gtcggtgggg tggtcgcgct
     9481 tgcgccgtgg tgggtgggcg gcgacgctga cctgatcccc gagtccaccc ggctgctggt
     9541 cgtgcacggc accgccgaca cctggaccga cccgcgcagt tcacggatcc agacgcggcg
     9601 ggccggcgag cacgggctgg acgcgcagtg ggtgggcgtg gcaggtgcga gacattacat
     9661 gctgcgccgc tgtggtgaat ggcaccgtcg cactacggac ttcgtcgccg actacctgag
     9721 ctgtagctaa tgggacccga acagcgagcc ttcgataggc ggtcccttgc tcgggggacg
     9781 ctgttaccga gccgccggtc ggtgagcgtc gtgagtagct gaagcgttag tcgacttcta
     9841 gagctagatc acgccccggc cggtt
//
LOCUS       DASSGQ010000099         4321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1222472,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000099 DASSGQ010000000
VERSION     DASSGQ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222472"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VFI55_04550"
     CDS             <1..689
                     /locus_tag="VFI55_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073337.1"
                     /translation="AVSADGYFAGVAAWGIALLAVAVHRPVRFPALIAAAAGLLLGWA
                     VFLSYGLALLGIPALAVLVSASDWRAALRALGPATLAALGVAVSFAVAGFYWFDGYTL
                     VQQRYWQGIAKYRPFQYWSWANLACVVCAIGLGSVAGISRVFDWAFDRAAVRRRSGFH
                     LLLVAMLAAIVFADLSMLSKAEVERIWLPFTVWLTAAPALLPVRSHRWWLALNAAGAL
                     LVNTVMVTNW"
     gene            complement(727..1389)
                     /locus_tag="VFI55_04555"
     CDS             complement(727..1389)
                     /locus_tag="VFI55_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HET7073338.1"
                     /translation="MSVLPVTVLVVAKSPEPGRAKTRLAATVGDRVAADIAAAALLDT
                     LDAVAAAPVAARVVAVAGDVGAAARAAELRRRLEAFTVIPQRGDDFANRLANAHVDCA
                     DGYPVLQIGMDTPQVTAELLTDCARRLLDAPAVLGMAHDGGWWVLGVATPTMAGCLRA
                     IPMSQPDTGELTLKALRDSGIDVAQVQSLADIDVLDDVAAVRAACGPASRFARVTRSA
                     GL"
     gene            complement(1386..2042)
                     /locus_tag="VFI55_04560"
     CDS             complement(1386..2042)
                     /locus_tag="VFI55_04560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7073339.1"
                     /translation="MGHDAVTVVLPCLNEEESLPAVLAAIPVGYRALVVDNNSTDDTA
                     AVATRHGARVVAETRPGYGAAVHAGVVAATTPTVAVIDADGSMDPGDLPRLVAALDRG
                     ADLVTGRRRPVPGLHWPWVARVGTVVMSWRLRTRHGLPVHDIAPMRVARREALLGLGV
                     VDRRSGYPLELLVRAAAAGWRVVEVDISYGPRTGGKSKVSGSLRGSITAILDFWKVIS
                     "
     gene            complement(2054..3763)
                     /locus_tag="VFI55_04565"
     CDS             complement(2054..3763)
                     /locus_tag="VFI55_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073340.1"
                     /translation="MDVVLGVAVAGPVARLALVGPGAHGADVIDQSVVDLADNPINTL
                     TQTVVGTNRLLADENHRLVATRLCWDDGKADQLRQSLEDSGVQNVAVLSQSQAVTALM
                     RAAGRAGGVLVVDDETATLSMVGGAEDGNAPPTLLASEPLGADATAALDTMMARLADL
                     PGAPTGLWLVGASSDLTGVADQLRDASTMQVEIPDDPTFALARGAALAAAPLAAASAA
                     DATAMAPAVGLTADDTAFAPAAEVAGAKAEEPQLAYSQADDGESLPGDEYGPEFDEYD
                     DFDEGADGAPARLSRRSLLIGNAVIAFAVIGFASLAAAVVIAVRPTASQVPVVGHQNA
                     APGKFMPLLPTQQQAPVPPPPPDAPNAGFQGGTVPAPTGGRSVPQQGPPPVAPAPVGP
                     ETPGTPGFVPNPNPNVPIPIPIIIPFPGWHPTNPYPNPYPYPYPPPTAPTTPYTPPTT
                     TQTPPTTTPPTTPPTTPPTTTHTPPTTTNAPPTTTNAPPTTTNAPPTTTYSPPTTQPS
                     TPKTTVPTSVAPPPSTKPPTQSVPTQTVPTQTHTQQQVTPQQPVSPQQPVSPQQPHGR
                     HGF"
     gene            4069..>4321
                     /locus_tag="VFI55_04570"
     CDS             4069..>4321
                     /locus_tag="VFI55_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073341.1"
                     /translation="MVSQSSDDAPTGPIRAHAQQTAAGGAGRRPVAAVTGRPTRRWNI
                     VSDLTSAALLVIALLLPWNLYFGFGIPGSNKTVLTVLFAV"
BASE COUNT          574 a         1474 c         1576 g          697 t
ORIGIN      
        1 tcgcggtttc ggccgacggg tacttcgccg gagtcgcggc atggggcatt gcgctgttgg
       61 ccgtggcggt gcaccgcccg gttcggttcc ccgcgctgat cgccgccgcg gccgggctgc
      121 tgctgggctg ggcggttttc ctcagctacg gcctggcctt gctcgggatt ccggcgctgg
      181 cggtgctggt gtcggcgtcg gattggcggg cggcgctgcg ggcactcggc ccggcgactc
      241 tggcggcgtt gggggttgcg gtgagcttcg ccgtcgcggg gttctactgg ttcgacggct
      301 atacccttgt gcagcaacgc tattggcagg ggatcgccaa ataccggccg ttccagtatt
      361 ggagctgggc gaacctggcg tgcgtggtgt gcgcgatcgg tctgggcagc gtggccggaa
      421 tcagccgggt gttcgactgg gcattcgata gagccgccgt ccggcggcgc tccggtttcc
      481 acctattgct ggtggcgatg ctggccgcca tcgtgttcgc tgatctgagc atgctgagca
      541 aggctgaggt cgaacggatc tggctgccgt tcaccgtctg gctcaccgcg gcgcccgcat
      601 tgctgccggt gcggtcacac cgctggtggt tggcgctcaa cgccgccggc gccttgctgg
      661 tgaacaccgt catggtcacc aactggtagg cgtcatcgag cgtgctgacg gctttcggtg
      721 tgaatgtcag agtccggccg agcgcgtgac ccgcgcgaag cggctcgcgg gtccgcatgc
      781 ggcgcgtacc gcggcgacat cgtccagcac gtcgatgtcg gccagcgact gcacctgggc
      841 cacgtcaatg ccactgtccc gcaatgcctt cagcgtgagt tccccggtgt cgggttgaga
      901 catcgggatg gcgcgcaggc atccggccat cgtcggcgtt gccaccccga gcacccacca
      961 accgccatcg tgcgccattc cgagcacggc cggggcgtcg agcaagcggc gtgcgcaatc
     1021 cgtcaatagt tcggcggtca cctgcggggt gtccatcccg atctgcagca ccggataccc
     1081 gtcagcgcag tcgacgtgcg cgttggcgag gcggttggcg aagtcgtcgc cgcgctgcgg
     1141 aatcaccgtg aaggcctcga gccgccgccg gagctcggcg gcgcgcgcgg cggcgcccac
     1201 gtcgccggca acggccacga cccgggccgc caccggcgct gcggccaccg cgtcgagcgt
     1261 gtccagcagc gcggcggcgg cgatgtcagc cgcgacccgg tcgccgaccg tcgccgcgag
     1321 ccgcgtcttg gcccggccgg gctccggcga tttggccacc accagcaccg tcaccggcag
     1381 gacgctcacg aaatgacctt ccagaagtcc aggatcgcgg tgatgctgcc gcgcagcgac
     1441 ccgctgacct tcgactttcc gccggtgcgg gggccgtagc tgatgtccac ctcgactacg
     1501 cgccagccgg cggccgcggc ccggaccagc agttccaggg gatagcccga gcgccgatcg
     1561 acgacaccga ggcccagcag cgcctcgcgt cgggctaccc gcatcggggc gatgtcgtgc
     1621 accggcaggc catgccgggt gcgcagccgc cagctcatca ccacggtgcc gacccgggcg
     1681 acccagggcc agtgcagccc ggggaccggc ctgcgccggc cggtgaccag gtcggcgccg
     1741 cggtcgagcg cggcgaccag ccggggcagg tcaccggggt ccatcgagcc gtcggcatcg
     1801 atgaccgcca cggtcggggt ggtcgcggcg acaacgcccg cgtgcaccgc cgccccgtac
     1861 cccgggcgcg tttcggcgac cacccgagcg ccgtgacgag tggccaccgc cgcggtgtca
     1921 tcggtgctgt tgttgtcgac caccagcgcc cgataaccga ccggaatggc cgccagtacg
     1981 gcgggcagcg attcttcctc gttgagacag ggcagcacca ccgtgaccgc gtcgtggccc
     2041 atggcgttcg cgatcagaag ccgtgccggc cgtggggctg ctgcgggctc accggctgct
     2101 gggggctcac cggctgctgg ggagtcacct gctgctgcgt atgcgtctgc gtcggaacgg
     2161 tttgcgtcgg aacactttgc gtcggtggct tcgtgctcgg cggcggcgcg acggacgtgg
     2221 gcaccgtcgt cttcggggtg gagggctggg tggtcggcgg cgaataggtc gtcgtcggcg
     2281 gggcgttcgt cgtcgtcggc ggggcgttcg tcgtcgtcgg aggggcgttc gtcgtcgtcg
     2341 gaggtgtgtg cgtcgtcgtc gggggcgtcg ttggaggtgt ggtcgggggc gtggtcgtcg
     2401 ggggtgtctg cgtggtggta ggcggcgtgt acggggtcgt cggcgccgtc ggcggtgggt
     2461 acgggtacgg gtacgggttg gggtaggggt tggtcgggtg ccagcccggg aacggaatga
     2521 tgatcgggat cggtatcggc acattagggt tcgggttcgg cacgaaaccg ggggtgcccg
     2581 gcgtttccgg cccgaccggc gctggcgcga cgggcggcgg tccctgctgc ggaaccgatc
     2641 tcccaccagt cggagcgggc acggtgccgc cctggaatcc tgcattgggg gcatcgggcg
     2701 ggggcggagg caccggcgcc tgctgttgcg tcggcaacag cggcatgaac ttgccgggcg
     2761 cggcgttctg gtgccccacc accggcactt gactcgcggt cggacgaacg gcgatcacca
     2821 ccgcggccgc cagcgacgcg aatccgatca ccgcgaaggc gatgaccgcg ttgccgatca
     2881 gcaacgatcg tcggctcagc cgcgccggtg cgccgtcggc tccttcgtcg aagtcgtcgt
     2941 actcgtcgaa ctcggggccg tactcgtcgc cggggagcga ttcgccgtca tcggcctgcg
     3001 aatacgccag ctgcggttcc tccgccttcg ctccggcgac ctcagccgcg ggcgcgaacg
     3061 cggtgtcatc tgcggtgagc cccaccgccg gggccattgc cgtcgcgtct gcggccgacg
     3121 ccgccgccag gggcgcggca gccagcgccg cgccgcgggc cagcgcaaag gtggggtcgt
     3181 cgggaatctc cacttgcatg gtcgacgcgt cgcgaagctg gtccgcaacc ccggtgagat
     3241 ccgacgacgc acccaccagc cagagccccg tcggggcgcc gggcaggtcg gcgagcctgg
     3301 ccatcatcgt gtcgagcgcg gcggtggcgt cagccccgag cggctcactg gccaacaggg
     3361 tgggtggggc gttcccgtcc tcggcgccac ccaccatcga cagcgtcgcc gtctcgtcgt
     3421 caaccacgag cacaccgccg gcgcgccccg cggcacgcat caacgccgtc acggcctgcg
     3481 actgcgacag caccgcaacg ttctggacac cggaatcttc cagcgactgc cggagttggt
     3541 cggccttccc gtcgtcccag cacagccggg tggcgaccaa ccgatggttc tcgtcggcca
     3601 atagccggtt cgtcccgacg acggtctggg tgagtgtgtt gatggggttg tcggccaggt
     3661 caacgacgga ttggtcgatc acgtcagcgc cgtgcgcgcc cggcccgacg agcgccaagc
     3721 gggcaacggg acctgcgacc gcgaccccca acacgacgtc catccccggc tctccttcgg
     3781 ctgggctacc ccacgaccag catgtcccgg catcattaca ctgcgatacc ctcggcagta
     3841 acagtcattc gggcgacagg gtttcgcctg tgttcgccta cgaccgcagc gtagccggat
     3901 tgcccaacag caaaaccgtc gacgcggaat cgcacgtccc ggcccctgcc gcgtcgggcg
     3961 aacggagatt atgtttccaa gtaagcaacg cagtcggcac gctagggggt tccctcgcct
     4021 cgcaacccgc gctgcgtggg ccgtagccgt ccgcgacacc cggggggaat cgtgagtcag
     4081 agcagcgacg acgcgcctac cggccccatc cgcgcgcacg cgcagcaaac ggcggcgggc
     4141 ggcgcgggac ggcggccggt cgcggccgtg acgggcaggc cgacccgccg gtggaacatc
     4201 gtcagcgacc tgacttcggc cgccctgctc gtcatcgcgc tgttgctgcc gtggaatctg
     4261 tacttcggtt tcggtatccc cggcagcaac aagaccgtct tgacggtgct gttcgcggtg
     4321 a
//
LOCUS       DASSGQ010000100         5639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_37459, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000100 DASSGQ010000000
VERSION     DASSGQ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37459"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VFI55_04575"
     CDS             <1..369
                     /locus_tag="VFI55_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET7073342.1"
                     /translation="LPLLVGRSRAIEIVLGADDYDAGTAERYGWINRAIADAELDDFV
                     ANFVRRVLSFDQQALSTAKKIINQRGLPDPAQLQATEDIFFTTYQWPGATERMPKLRE
                     RGIGTPGEFELNLGRHIENL"
     gene            complement(795..1091)
                     /locus_tag="VFI55_04580"
     CDS             complement(795..1091)
                     /locus_tag="VFI55_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007533950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HET7073343.1"
                     /translation="MSVSGLVGRVVGFLRAGYPDGVPATDYVPLFALLRRRLSDDEVR
                     QLARELSASGTLPVNVVDIEVAITKATDGLPSPEEVQRVKDRLVAGGWPVSDAL"
     gene            1273..1917
                     /gene="phoU"
                     /locus_tag="VFI55_04585"
     CDS             1273..1917
                     /gene="phoU"
                     /locus_tag="VFI55_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET7073344.1"
                     /translation="MRTAYHQQLEALNTRIADICGLTGQAMQDATDALLHADLALAED
                     VIDRHHEIAYRCSQLEADAFTVLALQAPVAGDLRRVVSALQDVADAHRMGALALHVAK
                     ISRRRHPGTAVPDEVSGYFAEMGRIAVRLGNDTKAAVLSRDPHQAAQLSVEDQSIDDI
                     HRQLFTIVSDHSWPHGTAAAVDVTLLSRYYERFADHAVEIARRVIYQATGAHQT"
     gene            complement(2155..2850)
                     /locus_tag="VFI55_04590"
     CDS             complement(2155..2850)
                     /locus_tag="VFI55_04590"
                     /inference="COORDINATES: protein motif:HMM:NF014089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HET7073345.1"
                     /translation="MKSAPRGCRSGSELHVCHGGDRLRLEPVGERSATKWRQYGCRGG
                     GLFMTEYCAHDRPGRVAFAAKLPGHIITVPLRPDTSLMVHRQGFLCGVGDIRLSRSFQ
                     RRLGAGVFGGTGFILQQLTGAGQAWVELSGEIVPYTLAPGETLRVHPRHVGMFEPSVS
                     FDVTMVRGIANALFGGDGLFMASLTEPGRVWLQTMTISNLAHAIAPYLPGRSEDSGGG
                     GMARTLGDQLSGS"
     gene            2940..4394
                     /locus_tag="VFI55_04595"
     CDS             2940..4394
                     /locus_tag="VFI55_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7073346.1"
                     /translation="MTLQREPTRAISRQTFLRGAVGALAAGAVFGSGRAIADPRTSGW
                     DGLSTTIGGQVIRPDSGQFAAAKQVFNTNYNDSTPAAVVVPTSAADVQKAMAFAAAHN
                     LKVAPRSGGHSYVGASTANGAMVLDLRQLPGGINYDAASGQVTVTPATSLYAIHEALA
                     AAGRGIPTGTCPSVGAAGHALGGGLGAQSRHAGLLCDQLMSASVVLPGGQAVTASSAN
                     NPDLFWALRGGGGGNAGVTTSLTFATFPTSDVDVVNLNFPAQSFAQVLLGWQNWLRTA
                     DQRSWALADATADAMGNHCRIMATCPAGSGNNVASAITSAVGTQPTGTENHTFNYLDL
                     VKYLAVGNLNPSPLGYVGGSDVFSTITPAAAQGIASAFDAFPHGAGRALVIMHALDGA
                     LASVAPGASAFPWRRQSSLVQWYVESSGNPSAATSWLNTAHQAVQPYSVGGYVNYLEA
                     NQPASRYFGANLSRLSAIRQKYDPGQIMFSGLNV"
     gene            4461..5231
                     /locus_tag="VFI55_04600"
     CDS             4461..5231
                     /locus_tag="VFI55_04600"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET7073347.1"
                     /translation="MVQTDPVLFSVDNRVALITVNDPGRRNALTDEMSRCLRDAVEHA
                     EADPDVHAVVITGAGKAFCAGADLSALGAAGGEAAESGLQRLYDGFMAVGACRLPTIA
                     AVNGAAVGAGLNLALAADVRIAGPGALFDARFQQLGLHPGGGATWMLQRAVGPQVARA
                     AVLFGMRFDAESAVRYGLALSVADDPVAAALELAAGPAAAPRDVVLATKATMRATASP
                     GSVDTEQHELAMRTELGPQATSIRSPEFAARLAAARRK"
     gene            complement(5269..>5639)
                     /locus_tag="VFI55_04605"
     CDS             complement(5269..>5639)
                     /locus_tag="VFI55_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET7073348.1"
                     /translation="ITDAQNRTTRELATRTAELIAAARGGTVTPADLRGSTFTVSNFG
                     ALGADDGVPVINHPEAAILGMGAIKPRPVAVGDQVVVRSTMTLTCVFDHRVADGAQAV
                     QFLRELRDLIESPETALLDL"
BASE COUNT         1035 a         1960 c         1743 g          901 t
ORIGIN      
        1 ctaccactgt tggtcgggcg ttcccgggcc atcgagatcg tcttgggcgc cgacgactac
       61 gacgcgggga ccgcggaacg atacggctgg atcaaccgtg ccatagccga cgccgagcta
      121 gatgacttcg tcgccaactt cgtgcgacgc gttctgtcct tcgatcagca ggctctgagc
      181 accgcgaaaa agatcatcaa tcagcgtggg ttgccggatc ctgcccagct ccaggcgacc
      241 gaagatatct tcttcacgac ataccaatgg ccaggggcta ctgaacggat gcccaagctg
      301 cgggagcgcg gcatcgggac tcccggcgaa ttcgaactga acctcggccg tcacatcgaa
      361 aatctgtagg caggcaccgg ctgcacccgt gctcggaagt gtctaggcga gcccgcgctt
      421 cgcgaacgac gtccagtgca tcggcagcat cgacgatgag gtgctgcgct agctctgcca
      481 ttccgctgcc cagcctggct acgctataca gctgtggcca ccaccgtcga cccacacgca
      541 gccctaggcg ccaaacagcc agtccgtcga tgaaaaccgc tgaggttcaa caagcccaga
      601 tgctcatcgc agaattacgt gacgcaatca aggaattgga agcccaaatc gagtccgccg
      661 agacccgctg ggcccaccgg ctgcaccgat cctcccgcga gatcgaaacc ccgccgcgac
      721 tagtgcggtt acgcactcag ctcgaccagg cgcaacgtct gctcacacgg ctacgttccc
      781 agcaagccaa acgctcaaag agcgtcgctg accggccaac ccccggcgac caaacggtcc
      841 ttgacccgct gcacttcctc cggtgacggc aacccatcag tggccttggt gatcgcgacc
      901 tcgatgtcaa cgacgttgac cggcaacgtt cccgaggcgc tcagttccct cgccagctga
      961 cggacctcat catcggaaag ccgccggcgc agcaaagcaa acagcggtac atagtcagtg
     1021 gcgggcaccc cgtcgggata gcccgcccgc aagaacccca ccacccgccc gacaagcccc
     1081 gacaccgaca tggctacacc tcaccgtcat tggtcaccgc accccgtgac cacgtcggtc
     1141 gtcgacctca tgcccccagg actgtccgtc gggggcgccc aagaatcgac cacacccgcc
     1201 actaccgggt aagtgttcta caccgggacc cgttgggcaa tcattgggcg cacactcgta
     1261 acctgagtct tcgtgcgcac cgcataccac caacagctcg aagctctcaa taccaggatc
     1321 gccgacatct gcgggctgac cggacaggcc atgcaggacg ccaccgacgc gctcctgcac
     1381 gccgacctcg cactcgccga ggacgtcatc gaccgccatc acgagatcgc ttaccggtgt
     1441 tcacagctgg aagccgatgc cttcacggtg cttgccctgc aagcacccgt tgccggtgat
     1501 ctacgcagag tggtcagcgc cctacaagac gttgccgatg ctcaccgcat gggcgccctt
     1561 gcactacacg tcgccaaaat cagccgccgc cgccaccccg ggaccgcagt gcccgacgag
     1621 gtcagcgggt acttcgccga aatggggcgc atcgcagtgc gactcggcaa cgacaccaaa
     1681 gccgctgtgc tatctcgtga tccgcaccaa gccgcgcagc tgtccgtcga ggaccaatcc
     1741 atagacgaca tccaccgaca gctgttcacc atcgtcagcg accacagctg gccacacggg
     1801 accgctgccg ccgtcgatgt cacgctgctc agccgctatt acgaacgttt cgccgaccac
     1861 gcagtcgaaa tcgcccgccg tgtgatctac caggccaccg gagcacacca gacctgaccg
     1921 aacaaagatc agctgccgct gagcttgtca tcgcgctgag ccaagccgcg acaatcccaa
     1981 acacgttgca ctataagaga gtaaagcaag gtgcgggtga cccggctacg gacgggtcgc
     2041 gtcgatctca gggactagtc aacggtgccg tagctgctcc ggacctaacc ccccgcgtaa
     2101 cgacggcgaa accgtaagcc cccgcccata attcgccgcc cttcggcgat atcgctagct
     2161 gccggagagc tggtcgccca gtgtcctggc catgccgccg ccacccgagt cctcggaacg
     2221 ccctggaaga taaggcgcta tcgcgtgggc caggttgctg atcgtcatgg tctgtagcca
     2281 gacgcgtccg ggttcggtaa gtgaggccat gaacaacccg tcgccgccga agagggcatt
     2341 ggcgattccg cgcaccatgg tgacgtcgaa ggagaccgag ggttcaaaca tgccgacgtg
     2401 gcggggatgt acccgcaggg tttctcccgg agccaatgtg tagggcacga tctcacccga
     2461 tagctccacc caggcctgcc cggcgccggt cagttgctgc aggatgaacc ccgttccgcc
     2521 gaacacgccc gccccgaggc ggcgttgaaa gctccgtgac agtcgaatgt cgccgacgcc
     2581 gcagagaaac ccttgccggt gaaccatcaa cgacgtatcg gggcgtagcg gcacggtgat
     2641 gatgtggccc ggaagtttcg cggcaaacgc gacgcggccg ggccgatcgt gcgcgcagta
     2701 ttcggtcata aacaagccgc cgccacgaca gccgtactga cgccactttg tggcggaacg
     2761 ctcccccacc ggttcaagcc tgagccgatc gccgccatgg cacacgtgta gttcgctacc
     2821 tgacctgcag ccgcgaggcg cggatttcac gagtcagggc ctgtcacaac ctgctaacag
     2881 tcccgcgaca cgtcgtgggc ggcggcggtt tttgatgccg agtagcccac actgcggaca
     2941 tgacgctaca gcgggagccg acgcgcgcga tctcgcgtca gacgtttctg cggggcgccg
     3001 tcggggcgtt agccgccggc gcggttttcg gatcgggccg agccattgcc gatccgagaa
     3061 cctcgggctg ggacggactt tccaccacca tcggcggcca agtgatacga ccggatagcg
     3121 gtcaattcgc tgcggccaaa caggttttca acactaacta caacgactcg acaccggcgg
     3181 ccgtcgttgt tcccacgtca gcggcggacg tgcagaaagc gatggcattc gccgccgcgc
     3241 acaacctcaa ggtcgctcca cgcagcggtg ggcactccta tgtgggggcg tccacggcga
     3301 acggcgcgat ggtgctcgac ctgcgccagc tgcccggcgg aatcaattac gacgccgcca
     3361 gcgggcaggt cacggtgacg cccgcgacca gcttgtatgc gatacacgag gcgctggccg
     3421 cggccggccg ggggatcccg acgggtacct gcccgtcggt cggtgcggcg ggccacgccc
     3481 tgggcggcgg gctgggcgcc cagtcccggc atgcgggcct gctgtgtgac caattgatgt
     3541 cggcgtcggt ggtgctgccc ggcggccagg cggtcacggc gtccagcgcc aacaaccccg
     3601 atctgttctg ggcgttgcgc ggcggcggtg gcggcaacgc cggggtgacc acgtcgctga
     3661 ccttcgccac ctttcccacc agcgacgtcg acgtcgtcaa cctcaacttc ccggcacagt
     3721 cattcgcgca ggttctcctc ggctggcaga actggctacg taccgccgac caacgcagct
     3781 gggcattggc cgacgccacc gccgacgcga tgggcaatca ctgtcgcatc atggcgacct
     3841 gcccggccgg gtcgggcaac aacgtggcga gcgcgatcac ctcggcggtg ggaacgcaac
     3901 cgaccgggac cgagaaccac acgttcaatt atttggatct ggtgaagtat ctggccgtcg
     3961 ggaacctcaa cccgtcgccg ctcggatatg tcggcggatc ggacgtattt tcgacgatca
     4021 cccccgctgc cgcccagggg atcgcctcgg cgttcgacgc ctttcctcac ggggcgggcc
     4081 gtgcgttggt gatcatgcac gcgctcgacg gcgccctcgc cagtgtggcg ccgggggcct
     4141 ccgccttccc gtggcggcgg cagtcctcgc tggtgcagtg gtacgtcgag tcatccggta
     4201 acccgtccgc agccaccagc tggctcaaca cggcacacca agcggtgcaa ccgtattcgg
     4261 tgggcggtta cgtgaattac ctggaggcga accaaccggc ttcgcggtac ttcggcgcga
     4321 acctgtcccg gctgtcggcc atccggcaga agtatgatcc gggtcagatc atgttctcgg
     4381 gtctcaacgt ctgacgcagg cctgagcggt cctgtctttc gttcgctccc gagaccgaac
     4441 ctggcaacgc ggaggtgccg atggtccaaa ccgaccccgt cttgttcagc gtcgacaatc
     4501 gtgtcgcgct catcaccgtc aacgaccccg gtcggcgcaa cgccctcacc gacgagatgt
     4561 cgaggtgcct gcgcgacgcc gtcgagcacg ccgaagccga tcccgatgtg cacgccgtcg
     4621 tgatcacggg cgcgggaaag gccttctgcg ccggcgcgga tctcagtgcc ctgggggctg
     4681 ccggaggcga agcggcggag tcgggactgc agcggctcta cgacggtttc atggccgtcg
     4741 gcgcttgccg attgcccacg atcgccgcgg tcaatggggc ggctgtcggc gcaggcctga
     4801 acctggcgct ggccgccgac gtccgcatcg ccgggcccgg tgcgttgttc gacgcccgct
     4861 tccaacagct gggcttacac cccggcgggg gcgcgacgtg gatgttgcag cgcgcggtgg
     4921 gtccgcaggt ggctcgcgcg gcggtgttgt tcggcatgcg cttcgacgcc gaatccgcgg
     4981 tgcggtacgg cttggcgctc agcgtcgccg acgaccccgt cgccgctgcc ctcgagttgg
     5041 ccgcgggccc cgcagcggct ccccgcgacg ttgtgctggc gaccaaggcc accatgcgtg
     5101 ccacggccag cccgggctcg gtggataccg agcaacacga acttgccatg cgcaccgagc
     5161 tggggccgca agcgacgtcg atccggtcgc cggaattcgc cgcccggttg gccgcggcac
     5221 gacgcaagta ggcggccgca cgcacgacac gccggacggt gggacggcct acagatccaa
     5281 cagggcggtc tcgggcgact cgatcagatc ccgcagttcg cgaaggaact gcaccgcctg
     5341 ggcgccgtcg gcgacacgat ggtcgaacac gcacgtcagc gtcatcgtcg accgcacgac
     5401 aacctgatcg ccgacggcca ccggccgagg cttgatcgca cccataccca gaatggccgc
     5461 ttcgggatgg ttgataaccg gcacaccgtc gtcggcgcct agcgcaccga agttcgacac
     5521 ggtgaacgtc gagccgcgca gatccgccgg cgtcactgtc cctccacgcg cagcggcgat
     5581 caattctgct gtccgggtag ccaattcgcg tgtcgtccga ttctgcgcgt cagtgatca
//
LOCUS       DASSGQ010000101        15113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1131375,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000101 DASSGQ010000000
VERSION     DASSGQ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15113
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131375"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VFI55_04610"
     CDS             complement(<1..879)
                     /locus_tag="VFI55_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7073349.1"
                     /translation="MTNDPRTVPAALDRLARRLPGHAALITDERSFTTAELRDEVHHA
                     AAALIALRVKPQDRVAIWSPNTWHWVVACLAIHHCGAVMVPLNTRYTATEANDILART
                     AAPVLFAMGQFLGNDRVAELDRDALPALRHVVRIPIDTAETGVGTWDEFIVQGSAAVG
                     VVDRAAAVSPDDVSDILFTSGTTGRSKGVLCAHRQSLSASASWAANGKITSDDRYLCI
                     NPFFHNFGYKAGILACLQTGATLIPHRTFDPLRALQAIEQHRITVLPGPPTIYQTLLD
                     HPARGDYDLSSLRFAVT"
     gene            979..2127
                     /locus_tag="VFI55_04615"
     CDS             979..2127
                     /locus_tag="VFI55_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7073350.1"
                     /translation="MQDVAEFRAEVRQWLADNLVGEFAELKGLGGPGREHEAFEERRA
                     WNQHLAAAGLTCLGWPVEHGGRGLPTAHRVAFYEEYARADAPVKINHFGEELLGPTLI
                     AFGTREQQQRFLPRILDVTELWCQGYSEPGAGSDLASVSTTAQLDGDQWVINGQKVWT
                     SYAHVSQWCFVIARTEKGFKRHAGLSYLLVPLDQPGVQVRPIVQITGASEFNEVFFDD
                     ARTDADLVVGAPGDGWRVAMGTLTFERGVSTLGQQIIYARELSRLAALAQSNGAADDP
                     FIRERLTRAWAGLRAMRSYALATMDNEQPGQDNVSKLLWANWHRSLGELAMDVVGKPG
                     MTMPDGEFDEWQRLFLFTRADTIYGGSNEIQRNIIAERVLGLPREAKG"
     gene            2124..2915
                     /locus_tag="VFI55_04620"
     CDS             2124..2915
                     /locus_tag="VFI55_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7073351.1"
                     /translation="MSILSEAPKEVAGHGLLQGKVVIVTAAAGTGIGSATARRALAEG
                     ADVVISDHHERRLGETADELSALGQGRVEKVLCDVTSTAQVDALIDSATARMGRIDVL
                     VNNAGLGGQTPVADMTDDEWDRVLDVTLTSVFRATRAALRYFRAAGHGGVIVNNASVL
                     GWRAQHSQAHYAAAKAGVMALTRCSAMEAVEYGVRINAVSPSIARHKFLDKTTSADLL
                     DRLSADEAFGRAAEPWEVAATIAFLASDYSSYLTGEVISVSSQHP"
     gene            complement(2928..4643)
                     /locus_tag="VFI55_04625"
     CDS             complement(2928..4643)
                     /locus_tag="VFI55_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015291547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPE domain-containing protein"
                     /protein_id="HET7073352.1"
                     /translation="MSFLVLPPEINSLRMFTGAGSAPMLGAAAAWTDLADELGTAADS
                     FSSVTSGLATQAWQGPASQAMTAAAAPYASWLSAASAQAANAAGQAQAVTSAFEAALA
                     ATIHPLAVTANRNTFVQLVMSNLFGFNAPAIAAAEGQYEEMWAQDVSAMVGYYGQASA
                     AAAQLPPLQALQSLPSLMGLAGSPTAAATPAAPNTTPGINLGVGNKGGSNLGSGNTGS
                     GNVGNGNTGNSNFGGGNVGDSNIGSGNNGGANIGLGNIGNRNVGFGNQGVPISGADPG
                     GGNQGFGNLGNSNAGFGNQGNSNQGAGNTGNANSGFANTGNSNIGIGNTGNNDIGIGL
                     TGNNQVGIGGLNSGSGNIGLFNSGTNNIGFFNSGSGNIGIFDAGTNTFTGGMNNFGIG
                     ESGIGNLGMGNSGFGNFGLGNSGIIDTGLFNTGATDTGMANAGANNTGNANSGSINTG
                     SGNSGNFNTGFFNSGNFNTGSGFVTDTPNTMVSGFGNTGTLVSGFFNTSSGGAFNGEM
                     SGFFNTASGGSVTDSDTSGFFNTGITAPIPIMGFPTSGLASGFNSGLLNMGTGLAGLF
                     NLIRL"
     gene            4911..5531
                     /gene="kstR2"
                     /locus_tag="VFI55_04630"
     CDS             4911..5531
                     /gene="kstR2"
                     /locus_tag="VFI55_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator KstR2"
                     /protein_id="HET7073353.1"
                     /translation="MDREPNPAISRRDELLELAATMFAERGLRATTVRDIADGAGILS
                     GSLYHHFSSKEEMVDEVLHSFLDWLFARYREIVDSEANPLERLKGLFMASFEAIEDRH
                     AQVVIYQDEAKRLSSQPRFAYIEDMNRQQRKMWVEVLNQGIDEGYFRPDLNVDLVYRF
                     IRDTTWVSVRWYRTGGPLTAEQVGQQYLAIVLGGITADSEKTDEGA"
     gene            5533..6693
                     /locus_tag="VFI55_04635"
     CDS             5533..6693
                     /locus_tag="VFI55_04635"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET7073354.1"
                     /translation="MPEAYVIDAVRTAVGKRNGSLAGIHPVDLGALAWRGLLDRSDVD
                     PMSVDDVIAGCVDAIGGQAGNIARLSWLAAGYPEEVPGVTVDRQCGSSQQAISFGAQA
                     IMSGTADLIVAGGVQNMSQIPISSAMTVGEQFGFTSPTNESKGWLHRYGDQKISQFRG
                     SELIAERWNLSREEMEQYALASHQRAFAAIRGGHFDNEIITVETEFGPFRIDEGPRES
                     SLEKMATLKPLVEGGRLTAAMASQISDGASAVLLASEQAVKDHKLTPRARIHHISARA
                     ADPVFMLTGPIPATRYALQKTGLSIDDIDTVEINEAFAPVVLAWLKEIKADPDKVNPN
                     GGAIALGHPLGATGAKLFTTMLNELERVGGRYGLQTMCEGGGTANVTIIERL"
     gene            6802..7710
                     /locus_tag="VFI55_04640"
     CDS             6802..7710
                     /locus_tag="VFI55_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013830633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HET7073355.1"
                     /translation="MTELFLGCEALAANAMPERVMRSLYEAVYPGVYGPSGIELTARQ
                     RAQAAWLWSRRKAVIAGNSAAALLGAKWVSPALDAELIHDNRRPPARITVHKDTLADG
                     EVGEVDDMPVTTPARTAFDIGRRTTSRLQALQRLDALTNATALKLTDVEGVIAEHPGA
                     RGLVRLRRVLPLVDGGAESPQETRSRLVLVEAGLPVPQTQITVLDDYGDFVARIDLGY
                     RDLRVGIEYDGPQHWTSAQQRDRDIDRYTALLDLGWTVIRVSSELLRYRRGTYVARVV
                     AAMRAAGWRPSLPNVNLATSSRRVAS"
     gene            complement(7732..8472)
                     /locus_tag="VFI55_04645"
     CDS             complement(7732..8472)
                     /locus_tag="VFI55_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073356.1"
                     /translation="MSIVAVLLIALGVADLCRRAIRVAWPPLVIGPVVVVVCAVLCAL
                     WRGGDIPLLVLAAVAAVVWEWLCLRSERSGKRQAAPLAVFAIALALLMVLSGWSSAVD
                     GIVAQWSAWVQLPLNHVTPTRLLMVVGVVLMQFATGNQLVRLVLGSVGAVRPPGEPQP
                     SDRLKGGRLLGPLERLLILGLGLGGQVAAASAVVAAKGIIRFPELNAQKSSNGEVGID
                     ELTEYFLVGSFASWLLALGGIALVLAAR"
     gene            complement(8469..9116)
                     /locus_tag="VFI55_04650"
     CDS             complement(8469..9116)
                     /locus_tag="VFI55_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HET7073357.1"
                     /translation="MALMKLDASPRATLLGDVVGSRRAADRSELHLVITKALRRVAAT
                     AIDPPAFTVGDEFQGAYATVGDALDAALVLRLTVSPEIDIRFGIGWGTVTILDADAGI
                     QDGPGWWAARDAIQHTAQVQRQPGFALVRTTFRAADDSRDDVAAINAALLCRDHLLGS
                     LDERSQRILKGLMNNRTKKELAADEGISPSAVSQRASRDGLDLIVLASQYLRSLA"
     gene            complement(9178..10248)
                     /locus_tag="VFI55_04655"
     CDS             complement(9178..10248)
                     /locus_tag="VFI55_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET7073358.1"
                     /translation="MRLRTPLTELVGVEHPVVQTGMGWVAGARLVSATANAGGLGILA
                     SATMTLDELATAIGKVKAATDKPFGVNIRADAADAGDRVELMIREGVKVASFALAPTQ
                     DLIARLKEAGAVVIPSIGAAKHARKVAAWGADAMIVQGGEGGGHTGPVATTLLLPSVL
                     DAMKATGIPVIAAGGFFDGRGLAAALSYGAAGVAMGTRFLLTSDSTVPDAVKRRYLEA
                     ALDGTVVTTRVDGMPHRVLRTGLVERLESGSRARGFAAAVGNAAKFKKMSRMTWRSMI
                     ADGMAMRHDKEMTWSQVVMAANTPMLLKAGLVEGNTKAGVLASGQVAGILDDLPSCAE
                     LIDSVVHDAIEQLRAASALVED"
     gene            complement(10245..10988)
                     /locus_tag="VFI55_04660"
     CDS             complement(10245..10988)
                     /locus_tag="VFI55_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET7073359.1"
                     /translation="MSTRAEVCAVACAELFRAAGEIVVSPMTNMAAIGARLARLTFSP
                     DILLTDGEAQLLADTPALGASGAVEGWMPFGRVFETLTWGRRHVVMGANQVDRFGNQN
                     ISAFGPLQHPTRQMFGLRGAPGNAINHATSYWVGNHSKRVFCEAVDVVCGIGWDKVDA
                     DNPAFRFTGTYRVVSNLGVFDFGGPQHTMRALSLHPGVSPNEVRENTSFEVHGLDAAE
                     VTRLPTDDELRLIRAAIDPKALRDKEVRS"
     gene            complement(10997..11881)
                     /locus_tag="VFI55_04665"
     CDS             complement(10997..11881)
                     /locus_tag="VFI55_04665"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET7073360.1"
                     /translation="MSNKLTTLDEAVMQLRSGMTIGIGGWGSRRKPMAFVRAMLRTDI
                     TDLTVVTYGGPDLGLLCSAGRVKRVYYGFVSLDSPPFYDPWFAKARTSGALEAREMDE
                     GMLRCGLQAAAQRLPFLPTRAGLGSSVPDFWEGELRTITSPYPAHGGGRETLIAMPAL
                     RLDAAFVHLNLGDSQGNAAYTGIDPYFDDLFLMAAEKRYLSVERVVSTEELIKAVPPQ
                     ALLVNRMMVDAVVEAPGGAHFTTAAPDYGRDETFQRHYAEAASSEEGWRQFVQTYLSV
                     GEDDYQAAVRSFGQQASS"
     gene            complement(11878..12621)
                     /locus_tag="VFI55_04670"
     CDS             complement(11878..12621)
                     /locus_tag="VFI55_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HET7073361.1"
                     /translation="MPITSTVTEPGIVAVTVDYPPVNAIPSRGWFELAEAVTAAGADP
                     DTHAVILRAEGRGFNAGVDIKEMQRTEAFSALIDANRGCFAAFRAVYECAVPVIAAVN
                     GFCVGGGIGLVGNSDVIVASDDATFGLPEVERGALGAATHLSRLVPQHMMRRLFFTAA
                     TVDAATLHHFGSVHEVVPRAELDEAALRVARDIAAKDTRVIRAAKEALNFIDVQRVNS
                     SYRMEQGFTFELNLAGISDEHRDAFVEKS"
     gene            12725..13477
                     /locus_tag="VFI55_04675"
     CDS             12725..13477
                     /locus_tag="VFI55_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7073362.1"
                     /translation="MNLGLAGRVVLVTGGVRGVGAGISAVFAGQGASVVTCARRSVEG
                     LPYEFHSCDVRDDDAVNTLINAIVDRHGRLDVVVNNAGGSPYMLTADSSAKFNRKIIE
                     LNLIGALAVSQHANDKMQNQAQGGSIVNICSVSGRRPTPGTAAYGAAKAGLESLTQTL
                     AVEWGPKVRVNACVVGMVETEQSELFYGDAESIAAISKNVPLRRLAKPDDVGWAAAFL
                     ASDAASYISGATLEVHGGGEPPHYLATTSAIK"
     gene            13492..14397
                     /locus_tag="VFI55_04680"
     CDS             13492..14397
                     /locus_tag="VFI55_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7073363.1"
                     /translation="MGVVDGRVVIVTGAGGGIGRAHALAFAAEGARVVVNDIGVGLDG
                     SPASGGSAAQGVVDEITAAGGEAVADGSDIADWDQAADLVATAVETFGGLDVLVNNAG
                     IVRDRMMANTSEDEFDAVIAVHLKGHFATMRHAASYWRGLSKEGKPVDARIINTSSGA
                     GLQGSVGQGNYSAAKAGIAALTLVGAAEMGRYGVTVNAIAPSARTRMTETVFAEMMAT
                     QDQDFDAMAPENVSPLVVWLGSAESRDVTGKVFEVEGGKIRVAEGWAHGPQIDKGARW
                     DPAELGPVVADLLAKARPPIPVYGA"
     gene            complement(14394..14852)
                     /locus_tag="VFI55_04685"
     CDS             complement(14394..14852)
                     /locus_tag="VFI55_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET7073364.1"
                     /translation="MKKPRNLNSPWADVFIKWMARANIWIYRRSNGTLGGTVQKAPVA
                     LLTTIGRKTGEPRVSPLIYVRDRDRVILVGSRGGSDKHPLWYLNLKADPKVSVQIKKE
                     VLHLRARDATAEERAEYWPKLAAVYPTLDDYRSWTDRVIPVVICDPGRTN"
BASE COUNT         2578 a         4894 c         5046 g         2595 t
ORIGIN      
        1 ggtcaccgcg aaccgcagcg agctcaggtc gtagtcaccg cgggcggggt ggtcgagcag
       61 ggtctggtag atggtgggcg gcccgggcag cacggtgatg cggtgttgct cgatggcctg
      121 cagcgcgcgc agcggatcga acgtccggtg cgggatcagc gtcgcgccgg tctgcaaaca
      181 ggccaggatg ccggccttgt aaccgaagtt gtgaaagaac ggattgatgc acaggtagcg
      241 gtcgtcgctg gtgatcttgc cgttggcggc ccacgatgcc gaggccgaca gcgattgccg
      301 gtgggcgcac agcacgcctt tgctacggcc ggtagtgccg gaggtgaaca gaatgtcgct
      361 gacgtcgtcg gggctgacgg cggcagcgcg gtcgacgacg cctaccgccg cagaaccctg
      421 gacgatgaac tcgtcccacg tcccgacacc agtctctgcg gtgtctatcg ggattcgcac
      481 gacgtgccgg agcgccggca gcgcgtctcg gtcgagctcg gcgacgcgat cgttgccgag
      541 gaattggccc atcgcgaaca gcaccggcgc tgcggtgcgg gccaggatgt cgttcgcctc
      601 cgtcgcggta taccgcgtgt tgagcggcac catgacagcg ccgcaatggt ggatcgccag
      661 gcaggcgacc acccagtgcc aggtgttcgg cgaccagata gccacccggt cttgcggttt
      721 gacgcggagc gcgatgagcg cggccgcggc gtggtgtacc tcgtcacgca attcggcagt
      781 ggtgaaggaa cgttcgtcgg tgataagggc cgcgtgcccg gggagtcggc gcgccaagcg
      841 atccagtgcc gcgggcacgg tgcgcggatc gttggtcact gatcctcctc acgcccgggc
      901 gcgcccctgc aagcggggac ccctacctca cgcggcggcg cttacaaagc aagtgcttgg
      961 taggttagcc tacagggcat gcaggacgtc gcggagttcc gggccgaagt ccgccagtgg
     1021 ctcgccgaca atctcgtcgg cgaattcgcg gagctcaagg gtctcggcgg accgggacgc
     1081 gagcacgagg cgttcgaaga gcgccgggcg tggaaccagc acctcgcggc ggccggactg
     1141 acctgtctgg gctggccggt tgagcacggc gggcggggcc ttccgaccgc acaccgggtg
     1201 gcgttctacg aggagtacgc ccgcgccgac gcgccggtaa agatcaatca cttcggggaa
     1261 gagctgctgg gaccgacgtt gatcgcgttc ggcacccgcg agcagcagca acgcttcctg
     1321 ccgcggatcc ttgacgtcac cgagctgtgg tgtcaggggt attcggaacc cggcgccggc
     1381 agcgacctgg ccagcgtgtc gaccaccgcc cagctcgacg gcgaccaatg ggtcatcaac
     1441 ggccagaagg tgtggacgtc atacgcgcac gtctcgcagt ggtgcttcgt gatcgcgcgt
     1501 accgaaaagg gctttaagcg ccacgccggt ttgtcgtatc tgctggtgcc gctggaccag
     1561 cccggcgtcc aggtccggcc catcgtccag atcaccggcg cctcggagtt caacgaggtg
     1621 ttcttcgacg acgcccgcac cgacgccgac ctggttgtcg gcgcgccggg cgacggttgg
     1681 cgcgtcgcga tgggaacgct gaccttcgag cggggcgtct ccacgctcgg gcagcagatc
     1741 atctatgcgc gtgagctttc caggctcgcc gcgctcgcgc agagcaacgg cgccgccgac
     1801 gacccgttca tccgggagcg gctgacgcgg gcgtgggccg gcctgcgcgc catgcgcagc
     1861 tacgctttgg ccaccatgga caacgagcag cccggacagg acaacgtgtc gaagttgttg
     1921 tgggccaact ggcatcggag cctcggtgag ctggcgatgg acgtggtcgg caagccgggg
     1981 atgacaatgc ccgatggcga gttcgacgaa tggcagcggt tgtttctgtt cacccgggcc
     2041 gacaccatct acggcgggtc caacgagatc cagcgcaaca tcatcgctga gcgcgtgctc
     2101 ggcctacccc gagaggctaa gggatgagta ttttgtccga agcgccgaaa gaggttgccg
     2161 gacacggcct tctgcaaggc aaggtggtaa tcgtgaccgc cgccgcgggc accggcatcg
     2221 ggtccgcgac cgcacggcgg gccctggcag agggcgccga cgtggtgatc tccgaccacc
     2281 acgaacgccg gctgggggag accgcagacg aactgtccgc gctgggacag ggccgtgtcg
     2341 agaaggtgtt gtgcgacgtg acgtccaccg cccaggtcga cgccctgatc gactcggcaa
     2401 ccgcgcggat ggggcgcatc gacgtgctgg tcaacaacgc cgggctgggc ggacagacgc
     2461 cggtggccga catgaccgac gacgaatggg accgcgtcct ggatgtgacg ctgacgtcgg
     2521 tgttccgggc cacccgagcc gcgctgcgct atttccgtgc cgctgggcac ggcggggtga
     2581 tcgtcaacaa tgccagcgtt ctgggctggc gcgcccagca ctcgcaagcg cactatgccg
     2641 cggccaaggc gggagtgatg gcgttaaccc ggtgcagcgc aatggaagcc gtcgagtacg
     2701 gagtccggat caacgcggtc tcgcccagca tcgcgcggca caagtttctg gacaagacga
     2761 cctcggccga ccttctcgat cggctatcgg ccgacgaggc gtttgggcgc gccgccgaac
     2821 cctgggaagt ggcggccacc atcgcgtttc tggccagcga ctactcgagc tacctcaccg
     2881 gcgaggtgat ctcggtatcc agccagcacc cgtgagctga ccagttgcta cagccggatc
     2941 aggttaaata accccgcgag accggtgccc atattcagca ggcccgagtt aaagccgcta
     3001 gcgaggccag acgtcgggaa gcccattatt ggtattggtg ccgtgatgcc ggtgttgaag
     3061 aaacccgacg tgtcgctgtc tgtgacggag ccgccgctgg ccgtgttgaa gaagcccgac
     3121 atttcgccgt tgaacgcgcc gccgctggac gtgttgaaga agcccgagac gagggtaccc
     3181 gtgttgccga agcccgagac catggtgttg ggggtgtcgg tgacgaaccc ggagcccgtg
     3241 ttgaagttgc ccgaattgaa gaagccggtg ttgaagttgc ccgaattccc gctgcccgtg
     3301 ttgatgctgc cggagttcgc attgcccgtg ttgttagcgc ccgcgttcgc catgccggtg
     3361 tccgtcgcgc ccgtgttaaa gaggccggtg tctatgatgc ccgagttgcc caagccgaag
     3421 tttccgaagc ccgagttccc cattcctagg ttgccgatgc ccgattcgcc gatgccaaag
     3481 ttattcatgc cgccggtgaa ggtattagtg cctgcgtcga agatgccgat gttgccgcta
     3541 ccggagttga agaagccgat gttgtttgtg cccgagttga acaagccgat gttgccgctg
     3601 cctgagttca gcccgccgat tcccacctga ttgttgccgg tgagcccgat cccgatgtcg
     3661 ttgttgccgg tgtttccgat cccgatgttg ctgttgccgg tgtttgcgaa cccgctgttt
     3721 gcgttgccgg tgtttccggc gccttggtta ctgttgcctt gatttccgaa gccggcgtta
     3781 gagttgccga ggttgccgaa gccttggtta ccgccgcccg ggtccgcgcc gctgatgggg
     3841 acgccctggt ttccaaagcc gacgttgcgg ttgccgatgt tccccaagcc aatgtttgcg
     3901 ccgccgttgt ttccgctacc aatgttggag tcgccgacgt ttccgccgcc aaagttggag
     3961 ttgccggtgt ttccgttgcc gacgttcccg ctaccggtgt tcccgctgcc caggttggag
     4021 ccgcccttgt taccgacacc caggttgata cccggggtcg tgttgggcgc cgcaggcgtg
     4081 gcggccgcgg tcgggctgcc cgccaggccc atgaggcttg gcaggctctg cagcgcttgc
     4141 aatggcggca gttgggcggc cgccgccgac gcctgaccgt agtagcccac cattgcggac
     4201 acgtcctgag cccacatctc ctcgtactgg ccctcagcag ccgcgatcgc cggcgcgttg
     4261 aagccaaaca agttcgacat caccagctgc acgaacgtat tgcggttggc cgtcaccgcg
     4321 agcgggtgga tcgtcgccgc caaggccgcc tcaaacgcac tggtcaccgc ctgcgcctgt
     4381 ccggccgcgt tcgctgcctg ggccgaggcc gcgctcagcc agctcgcgta aggcgccgcc
     4441 gcggccgtca tcgcctgcga cgccggcccc tgccatgcct gagttgccag ccccgaggtc
     4501 accgacgaaa acgaatccgc tgctgttccc agctcgtcgg ccaggtcggt ccacgccgcc
     4561 gcagccccca gcatgggtgc agaaccggca ccggtaaaca tccgcaagga attgatctct
     4621 ggcggtaaca ccaagaaact catggctgtt gacctccaca ctgagagccg tttgtcggta
     4681 gtcgggctgc ccccaacgcg agtgagacta cgacgattcg ggggattgct ctcggttttg
     4741 gccgataatc tcaagacaga ccacgcgggg acggctttcg ttggtccaga atgaacgctg
     4801 ccggcgcccg tgtccgccat gaggcggtga tgccgtagcg cgacatgtct gggattcggg
     4861 cgcgcccatc acggctggcc gagcaagcgc ttggttgata ctctgatcgg gtggaccgcg
     4921 agcctaaccc ggcgatcagc cgccgcgacg agttgctgga gctcgccgcg accatgttcg
     4981 ccgagcgtgg cctgcgcgcc accaccgtgc gcgacatcgc cgacggagcc ggcatccttt
     5041 ccggcagcct ctaccaccat ttctcttcca aggaggagat ggtcgacgag gtgctgcaca
     5101 gttttctcga ctggctgttc gcccgctacc gcgaaatcgt tgacagcgaa gccaatccgc
     5161 tggagcgact caagggactg ttcatggcgt cgttcgaggc gatcgaggat cggcatgcgc
     5221 aggtcgtgat ctatcaggac gaagccaaac ggctgtcatc gcagccccgg ttcgcctaca
     5281 tcgaggacat gaaccgccaa cagcgcaaga tgtgggtcga ggtgctcaat cagggcatcg
     5341 acgagggcta cttccggccc gatctcaacg ttgacctcgt ctaccgattc atccgtgaca
     5401 ccacctgggt gtcggtgcgc tggtatcgga ccggcgggcc gctcaccgcg gagcaggtgg
     5461 gtcagcaata tctcgccatc gtgctcggcg ggattactgc ggattctgaa aagaccgacg
     5521 agggagcgtg aaatgcccga ggcgtacgtc atcgatgctg tacgtaccgc ggtcggcaag
     5581 cgcaatggat cgctggcagg catacacccc gtcgacctgg gtgcgttggc gtggcgcggg
     5641 ttgctcgacc ggtctgacgt cgaccccatg tctgtcgatg atgtgatcgc cggttgcgtc
     5701 gacgcgatcg ggggacaggc gggcaacatc gcccggctct cgtggctggc cgccggctat
     5761 cccgaagagg ttcccggcgt cacagtcgac cggcagtgcg ggtccagcca acaggcgatt
     5821 tcctttggtg cgcaggcgat catgtccggc acggccgacc tgatcgtggc cggcggcgtg
     5881 cagaacatga gccagattcc gatctcgtcg gcgatgacgg tcggcgagca gttcggcttc
     5941 acgtcgccga ccaatgagtc caagggatgg ctgcaccggt acggcgatca gaagatttcc
     6001 caattccgcg gctccgagct aatcgccgag aggtggaacc tgtcccgcga agagatggag
     6061 caatacgcgc tggccagcca ccagcgcgcg ttcgcggcga tccgcggcgg ccacttcgac
     6121 aacgagatca tcactgtgga aaccgaattt gggccgtttc ggatcgacga gggcccgcgc
     6181 gaatcgtcac tggaaaagat ggccaccttg aagccgttgg tcgagggggg tcggctgacc
     6241 gcggcgatgg ccagtcagat ttccgacggc gccagtgcgg tgctgctcgc ctcggagcag
     6301 gccgtcaagg accacaagct gacgccccgg gcgcgcatcc accacatcag tgcgcgcgcc
     6361 gccgatccgg tgttcatgtt gaccgggccc atcccggcca cccgctacgc gctgcaaaag
     6421 accggactga gcatcgacga catcgacacc gtcgagatca atgaggcgtt cgcgccggtc
     6481 gtgctggcct ggctcaagga gatcaaggcc gatccggaca aggtcaaccc caacggcggc
     6541 gcgatcgccc tcggccatcc gctgggcgcc accggggcca agctgttcac caccatgctc
     6601 aacgagttgg agcgcgtcgg cggccggtat ggtctgcaga cgatgtgcga gggcggcggc
     6661 accgccaacg tcacgatcat cgagcggctg taactccctg cgccctgcgc cgaatgtgaa
     6721 cctcgcgacg tcctctcggc gtgtcgcgtc gtaaattgca cactcgcggc cctgtcagtg
     6781 gcccgcggca gcatccctgg catgacggaa ctctttttgg gctgtgaagc cttagccgcc
     6841 aatgccatgc cggagcgggt gatgcgttcg ctctatgagg cggtgtaccc aggcgtatac
     6901 ggccccagcg gcatcgagtt gacggcacgc cagcgagcgc aggcggcatg gctgtggtcg
     6961 cggcgcaagg cggtcatcgc aggcaattcg gcggcggcac tacttggcgc caagtgggtg
     7021 agcccggcgc tcgacgccga gctcatacac gacaatcgcc gcccgccggc gcgcatcacg
     7081 gtgcataagg acacgctggc cgacggcgag gttggcgaag tcgacgacat gccggtaacc
     7141 acgccggcac gtacggcatt cgatatcggg cgccgaacga cctctcggct gcaggcactc
     7201 cagcgacttg acgcattgac gaatgcgacc gcgctaaagc tcacagatgt ggagggggtc
     7261 atcgccgaac atccgggcgc gcgtggactg gtccggctcc gacgggtctt gccgcttgtc
     7321 gacggaggtg cggaatcgcc acaggagacg cgaagccggc tggtgttggt cgaggcgggc
     7381 ctgcctgtgc cccagacgca gatcacggtc ctcgacgact acggtgactt cgttgcgcgc
     7441 attgacttgg gttacaggga tttacgggtc ggcatcgagt acgacggccc tcagcactgg
     7501 acgagcgcgc agcagcgcga ccgcgacatc gaccgctata ccgcattgct cgatctgggc
     7561 tggaccgtga tcagggtcag tagcgagcta ctccgatacc ggcgaggcac gtacgtcgcc
     7621 cgcgtcgtcg ctgccatgcg tgctgccggg tggcgcccct ctttgccgaa tgtgaacctc
     7681 gcgacgtcct ctcggcgtgt cgcgtcgtga attgcacact cgcgcaaccc gttaccgcgc
     7741 agccagcacc agcgcgatgc cgccaagcgc cagcagccaa ctcgcgaagc tgcccaccag
     7801 gaagtactcg gtcaactcat cgatgccgac ctcaccgttg ctgctttttt gggcgttcaa
     7861 ctcgggaaac cggatgatgc cctttgcggc gacgacggcg ctggccgcgg ccacctgtcc
     7921 gcctaggccg aggcccagga tcagcaggcg ttccagaggg ccgagcaggc ggcctccctt
     7981 gaggcggtcc gatggctgcg gttcgccggg aggacgtacc gcgcccactg atccgagcac
     8041 gaggcgcacc agctgattgc cggtggcgaa ctgcatcagc accacaccca cgaccatcag
     8101 cagtcgagtg ggcgtgacgt ggttgagcgg cagctgtacc caggccgacc actgcgccac
     8161 gatgccgtcg accgccgaag accatccgga caacaccatc agcagcgcca gcgcgatggc
     8221 gaacacggcc aggggcgcgg cttgccgctt gccgctgcgt tcgctgcgca ggcatagcca
     8281 ctcccagaca accgcggcga cggccgctag gacgagcaag gggatgtcgc cgccacgcca
     8341 cagggcacac agcaccgcac acaccaccac cacgaccggt ccgattacca gcgggggcca
     8401 cgccacgcga atcgcccgtc gacacaggtc ggccacgccc agcgctatga gcaaaaccgc
     8461 gacgatgctc acgccagact ccgaagatat tggctggcaa gcacaatcag atccaagccg
     8521 tcgcggctag cgcgctggga caccgccgag ggactgatgc cctcgtcggc ggccagttcc
     8581 tttttggtcc ggttgttcat caagcccttc aaaatccgct gtgagcgttc atcgagtgat
     8641 ccaagcagat ggtcccgaca cagcagggcc gcgttgattg cggcgacgtc gtcacggctg
     8701 tcgtcggccg cccgaaacgt cgtgcgaacc agcgcgaagc cgggctgccg ctgcacctgt
     8761 gcggtgtgtt ggatggcgtc tcgcgcggcc caccagccgg gtccgtcctg gattcccgca
     8821 tcggcgtcga ggatggtgac cgtgccccag ccgatcccga accggatgtc gatttcgggg
     8881 ctcacggtca gccgcagtac cagcgcggca tcgagcgcgt cgcccacggt cgcgtaggcg
     8941 ccctgaaact cgtcgccgac ggtgaatgcc ggcgggtcga tcgcagtggc ggcgacgcgg
     9001 cgcagggctt tcgtgatgac caggtgcagc tcggaccggt cggccgcgcg ccgtgatccg
     9061 acgacgtcgc cgaggagcgt cgcccgcggt gaagcatcaa gcttcatcaa agccatgtga
     9121 agaatatagc ttaagtacga cgaattttag ccctgggctt cagtaaaggc ggccacgcta
     9181 gtcctcgacc agtgcagacg ccgcccgtag ctgctcgatc gcgtcgtgaa cgaccgaatc
     9241 gatcagctcc gcacacgacg gcaggtcgtc gaggatgccg gccacctgtc cggaggccag
     9301 gaccccggcc ttggtattgc cttcgaccag cccggctttc agcagcatcg gggtattcgc
     9361 cgccatcacc acctgcgacc aggtcatctc cttgtcgtgg cgcatcgcca tgccgtcggc
     9421 gatcatcgag cgccaggtca tccgcgacat cttcttgaac ttcgcggcat tacccaccgc
     9481 cgcagcgaaa ccccttgccc gcgagccgct ttccagcctt tcgaccaatc cggtgcgcag
     9541 cacgcggtgc ggcatgccgt cgacccgcgt ggtgaccacg gtgccgtcca gcgccgcctc
     9601 cagatagcgc cttttcaccg cgtcgggcac cgtggagtcc gaagtgagca gaaaccgggt
     9661 acccatcgcc acgcccgcgg ctccatagga caaggccgcc gcgagcccgc gtccgtcgaa
     9721 gaaaccgccc gccgcgatca ccgggatccc ggtggccttc atcgcgtcca gcacggacgg
     9781 cagcagcagc gtcgtggcga cgggcccggt gtgtccgccg ccctcgccgc cctgcacgat
     9841 catcgcgtcg gcgccccagg ccgccacctt gcgcgcgtgt ttggccgcgc cgatcgacgg
     9901 gatcaccact gccccagcct ctttcagccg ggctatcagg tcctgcgtgg gcgccaaagc
     9961 gaacgatgcc accttcacgc cctcgcggat catcaactcg acgcggtcac cggcgtcggc
    10021 ggcgtcggcg cggatgttca cgccgaacgg cttgtcggtg gcggccttga ccttgccgat
    10081 ggccgtcgcc agctcgtcca gcgtcatggt cgccgaagcc aggatgccga ggccgcccgc
    10141 gttggccgtg gcagacacca gccgggcgcc ggcgacccag cccatcccgg tttgcaccac
    10201 cgggtgctcg acgccgacca gctcggtcag cggcgtgcgc aacctcatga acgtacctct
    10261 ttgtcgcgca acgccttcgg atcgatagcc gcgcggatca ggcgcagctc gtcgtcggtg
    10321 ggcagccggg tcacctcggc cgcgtcaagg ccgtgcacct cgaacgaggt gttttcgcgg
    10381 acttcgttgg gtgacacgcc ggggtgcagg ctcagtgccc gcatggtgtg ctgtgggccg
    10441 ccgaagtcga atacgcccag attcgacacc actcggtagg tgccggtgaa ccgaaatgcg
    10501 gggttgtccg cgtccacctt gtcccagccg atgccgcaga ccacgtcgac ggcctcgcaa
    10561 aacacccgct tcgagtggtt gccgacccag taactggtgg cgtggttgat ggcgttgccg
    10621 ggcgcgccgc ggaggccgaa catctgccgg gtcgggtgct gcagcgggcc gaatgccgaa
    10681 atgttctgat tgccgaaacg gtcaacctga ttggcgccca tcaccacatg gcgccgcccc
    10741 cacgtcagcg tctcgaacac ccgtccgaat ggcatccagc cttcaacggc tccggacgca
    10801 cccagtgccg gggtgtccgc caggagctgc gcctcaccgt cggtcagcag gatatcgggc
    10861 gagaaagtga ggcgcgccaa ccgggcgccg atggcggcca tgttcgtcat cgggctgacc
    10921 acgatttcgc ctgccgccct gaacaattcg gcgcaggcga ccgcacacac ctcggcccgg
    10981 gtgctcaccg cgttggtcac gacgatgcct gctgtccgaa tgaacgcacc gcagcttggt
    11041 agtcgtcttc gccgaccgac agataggtct ggacgaactg ccgccagccc tcttccgacg
    11101 aggccgcttc ggcgtagtgc cgctggaacg tctcgtcacg gccgtaatcg ggtgcggcgg
    11161 tggtgaagtg ggcgccgccg ggcgcctcca caaccgcgtc gaccatcatc cggttcacca
    11221 acagtgcctg tggcggcacc gccttgatca gctcttcggt cgacacgacg cgctccaccg
    11281 acaggtagcg cttctcggcg gccatcaaaa agagatcgtc gaagtagggg tcgatgcccg
    11341 tgtacgccgc attgccttgg ctgtcaccga gattgaggtg cacgaaggcg gcgtccaggc
    11401 gcaacgccgg catggcaatc agcgtctcgc gtccgccacc gtgagccgga tagggactgg
    11461 tgatggttcg cagctcgccc tcccagaagt ccgggaccga gctgcccagc ccggcacgcg
    11521 tcggcaggaa cggcaggcgt tgagccgcgg cttgcagccc gcagcgcagc atgccctcgt
    11581 ccatttcccg ggcttcgaga gctccgctgg tgcgggcctt ggcgaaccac ggatcgtaga
    11641 agggcggcga atccagcgag acgaacccgt agtagacgcg cttgaccctg ccggccgagc
    11701 acaacagtcc aaggtcgggt ccgccgtagg tgaccactgt caggtccgtg atgtcggtgc
    11761 gcagcatggc ccgcacaaag gccatgggtt tgcgccgcga cccccagccg ccgatgccga
    11821 tcgtcatgcc gctgcgcaat tgcatgacgg cctcgtcaag ggtcgtcaac ttattgctca
    11881 tgacttttcg acgaacgcgt cgcggtgctc gtcggatatt ccggcgaggt tgagctcgaa
    11941 cgtaaagcct tgctccatac ggtaactcga gttgacccgt tgcacgtcga tgaagttgag
    12001 cgcctctttg gccgcacgga tcacgcgcgt gtctttggcg gcgatgtcgc gggccacccg
    12061 cagagcggct tcgtcgagtt cggcgcgggg caccacctcg tgcacggagc cgaagtggtg
    12121 caaggtggcg gcatccacgg tcgccgcggt gaagaacagc cgtcgcatca tgtgttgggg
    12181 caccagtcgc gacaggtgag tcgccgcacc cagcgcgccg cgctccacct ccggcagccc
    12241 gaaggtggcg tcgtcggagg cgacgatgac gtcggagttg cctaccaggc cgatgccgcc
    12301 gccgacgcag aatccgttca ccgccgcgat caccgggacc gcgcactcgt agaccgcgcg
    12361 gaacgcggcg aagcagccgc gattggcgtc gatgagcgcg ctgaatgctt cagtccgctg
    12421 catctccttg atgtcgacac cggcgttaaa gccgcggccc tcggcccgca ggatcacggc
    12481 gtgggtgtcg ggatcggcgc ccgccgcggt gaccgcttcc gcgagttcga accagccacg
    12541 cgacgggatg gcgttgacgg gcgggtagtc gacggtgacc gcgacgatgc ctggttcggt
    12601 cacggtggat gtgatcggca aggtggcagg tccttcccaa aatacgtggc tacctaagca
    12661 agcacttgct tggtacgcta gcacagtgac tcgccccgaa ggaggccccc ttcctggaga
    12721 agccatcaat ttgggcctgg cagggcgggt tgttctggtc accggcggcg ttcgaggggt
    12781 aggcgccggt atcagtgcgg ttttcgccgg gcagggtgcc agcgttgtca cctgtgcccg
    12841 gcgttccgtc gaaggcctgc cgtatgagtt tcattcgtgc gacgtgcgcg acgacgacgc
    12901 cgtcaacaca ctgatcaacg cgatcgtcga ccggcacggc aggctcgacg tcgttgtgaa
    12961 caacgccgga ggttccccat acatgctcac cgcggattcc agcgcgaagt tcaaccgcaa
    13021 gatcatcgag ctcaatctga ttggtgcact ggctgtttcg cagcatgcga acgataagat
    13081 gcaaaaccag gcgcagggcg ggtcgatcgt caacatctgc agtgtcagcg gacgacggcc
    13141 gaccccgggc accgccgcgt acggagcggc caaggcgggt ctggagagcc tcacgcagac
    13201 actcgcggtg gagtgggggc cgaaggtcag ggtgaatgcg tgcgtggtcg gcatggtgga
    13261 gaccgaacag tcggaactgt tctatggtga cgccgagtcg atcgctgcga tttcgaagaa
    13321 tgtgccgttg cgccggctgg ccaaacccga cgatgtcggt tgggccgcag cgtttttggc
    13381 ctcagatgcg gcgtcttaca tcagcggtgc cacgttggag gtgcatgggg gcggcgagcc
    13441 accgcactat ctcgccacca cgagcgcgat caagtagagg agacaagggg tatgggagtg
    13501 gtggacggcc gcgtcgtcat cgtcaccgga gcgggcggtg gcatcggtcg cgcgcatgcc
    13561 ctcgccttcg cggccgaagg cgcgcgtgtg gtggtcaacg acatcggggt gggcctggac
    13621 gggtcgcccg caagcggcgg cagcgccgcg cagggcgtgg tggacgagat caccgcggcc
    13681 ggtggggaag ccgtcgccga cggatccgac attgccgact gggatcaagc ggccgacctg
    13741 gttgcgaccg ccgtcgaaac tttcggcggc ctagacgttt tggtgaataa cgccggcatc
    13801 gtgcgggacc ggatgatggc caacaccagc gaggatgagt tcgacgccgt gattgcggtg
    13861 cacctcaagg gccacttcgc cacgatgcgc cacgccgcgt cgtattggcg tggactctcc
    13921 aaagaaggga aaccggtcga cgcacggatc atcaacacca gttcgggtgc gggcctgcag
    13981 ggcagcgtcg ggcagggtaa ctacagcgcc gccaaagcag gtatcgctgc gctgacgctc
    14041 gtcggcgccg cggagatggg tcgttacggc gtcaccgtca acgcgatcgc gccatcagca
    14101 cgcacccgga tgaccgagac ggtgtttgcg gagatgatgg caacgcagga ccaagatttc
    14161 gacgccatgg caccggaaaa cgtttcgccg ctggtggtct ggctgggcag cgccgaatca
    14221 cgggacgtca ccgggaaggt attcgaggtc gagggtggca agatccgcgt cgcggagggc
    14281 tgggcgcatg gcccgcagat cgacaagggt gccagatggg acccggccga gctgggaccg
    14341 gtggtcgccg acctgctggc gaaggcgcgg ccaccaattc ccgtctacgg cgcctagttg
    14401 gtccgcccgg gatcgcatat cacgaccggg atcacccgat cggtccacga ccggtagtcg
    14461 tcgagggtgg gatatacggc ggccaacttg ggccagtact ccgctcgttc ttcggccgtg
    14521 gcgtcgcgtg cccgcaaatg gagcacttct tttttgatct gcaccgagac tttcgggtcg
    14581 gctttgaggt tgaggtacca cagcgggtgt ttgtccgacc cgccccgcga ccccaccaga
    14641 atcacgcgat cgcggtcgcg cacatagatc agcggactca cccgcggctc accggtcttg
    14701 cggccgatgg tggtcagcag tgcgaccggg gccttctgga ccgtcccgcc gagcgttccg
    14761 ttgctccggc gataaatcca tatgttggcc cgggccatcc acttgataaa aacgtcggcc
    14821 cagggcgagt tcaaattgcg tggctttttc atttgagtcc cggtagtcag gtttgattgc
    14881 ccggctccgt ctcaccccgc tgcgcgggct gcatcgtcgc cggagggtgc tggattgccc
    14941 gactccttct caccccgctg cgcgggctgc atcgtcgccg ggcgttgctg gattgcccga
    15001 ctccttctca ccccgctgcg cgggctgcat cgtcgccggg cggtgccgga tgaatggtcg
    15061 gaaggtaacc ttgatgtggt ggatttgcgc ttgcccgctc ggactttcgg cgg
//
LOCUS       DASSGQ010000102         3446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1131457,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000102 DASSGQ010000000
VERSION     DASSGQ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131457"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..909)
                     /locus_tag="VFI55_04690"
     CDS             complement(94..909)
                     /locus_tag="VFI55_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7073365.1"
                     /translation="MSINRLQAHWGFTRMPFGRSLAPSMLHRHDGHAEAVARIGWCID
                     QHALGVITGEVGAGKTVAVRAATAALDTSRHVVIYLPNPSVGVRGMLHHVVSALGQVP
                     SFYTATLVPQAAAALAAEHAERGRTPIVIFDEAHLLDNAQLEAVRMLTNHEMDSGAPF
                     AALLVGQPTLRHRLRLGVLAALDQRISVRYALAGMTATETADYITHHVKIAGRTDTLF
                     SHDAVTLIHNASRGYPRAINNLAVNALTSAFARNSPTVDEKAARIAISETGAD"
     gene            complement(906..2411)
                     /locus_tag="VFI55_04695"
     CDS             complement(906..2411)
                     /locus_tag="VFI55_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011600390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HET7073366.1"
                     /translation="MTTYEHDDDAARRARAQQVALFRYQLICPALDPGLSTKARGRVV
                     RAIAAGTHAGPFGGQYGYSRDTLDRWIRRYRVGGFDALTPSTRQPGSRIDTTVLELAV
                     ALKRENPERTAAQVARILRASSGWAPSESTLLRLFHRRELMGPAAGDEPAVFGRFEAA
                     APNERWVGDALHGPRLGGRKTYLFAFLDDHSRLAVGYRFGFAEDTVRLAAALQPALAA
                     RGVPASCYVDNGSAYVDSWLLRACGKLGIRLIHSTPHRPQGRGKIERWFRGVRDQFLV
                     EVTGTTAEQLAEQGLSPAAALLELNALFTAWVESVYHHSVHSETGQTPLRRWDDGWQA
                     AGHGPAMASADALTEAFLWSQWRVVTKTATVSLHGNTYQVEPVLVGRKVELVFSPFNL
                     EVIDVHYQDAHYGRAVPHRITRHAHPKARPETPEPAQAPATGIAYLQLVADAHQQQVA
                     ADERIGFHALYSTGQTGTQASPPDSDQLAGQLSIDDAVIDDVTQPRAEAGA"
     gene            complement(2484..3116)
                     /locus_tag="VFI55_04700"
     CDS             complement(2484..3116)
                     /locus_tag="VFI55_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7073367.1"
                     /translation="MVTVEADPVGVESRLMGGRVDCPDCSGALRPWGWARPRRVHGIG
                     GVLRPRRGRCGGCLVTHVLLPVTVLLRRAYAADVIGAALLARAAGRGHRSSGAALEVP
                     AATVRGWLRVTAGRLEAVRVRLLQVAVRAGVDVRVPERLGCRWRDLLAALGAATAAVT
                     GRFGPVGVLGAVTAWQVAVACSAGRLLAPGWPVAGGRQPATRVAPDVRGG"
BASE COUNT          521 a         1217 c         1211 g          497 t
ORIGIN      
        1 gtggtgcgga agggccccgt ccgctggttg tggacggggg cttggtcggt tcggtgccgg
       61 ggtactcggc gtggtggtgg tggtggtggc gggtcagtcg gcgcctgttt cggagatcgc
      121 gatgcgggcg gctttctcgt cgacggtggg gctgttgcgc gcgaacgctg aggtgagcgc
      181 gttgacggcc aggttgttga tcgcgcgtgg gtagccgcgg gaggcgttgt ggatcaaggt
      241 gaccgcgtcg tggctgaaca gcgtgtcggt ccggccggcg atcttcacgt ggtgcgtgat
      301 gtagtcggcc gtctcggtgg cggtcatgcc ggccagggcg tagcggacgg agatccgctg
      361 gtccagcgcg gcgagcacgc cgaggcgtag ccggtgccgc agcgtcggtt gcccgacgag
      421 cagcgcggcg aacggcgcgc ctgagtccat ctcgtggttg gttagcatgc gcaccgcttc
      481 gagttgggcg ttgtcgagca ggtgcgcttc gtcgaagatc acgatcgggg tgcggccccg
      541 ttcggcgtgc tcggcagcca gcgccgccgc ggcctgtggg accaacgtcg cggtgtagaa
      601 gctgggcacc tgcccgagtg cggacacgac gtggtgcagc atgccgcgca ccccgactga
      661 cgggttgggt aggtagatca ccacgtgccg ggaggtgtcc aacgctgcgg tggcggcccg
      721 gacggccact gtctttcccg cgccgacttc gccggtgatc acgcctaggg cgtgctggtc
      781 gatgcaccag ccgatccggg cgacggcttc ggcgtgcccg tcgtggcggt gcagcatcga
      841 cggggcaagg cttctgccga acggcatcct cgtgaaaccc cagtgcgcct gcagacgatt
      901 gatgctcatg cccccgcctc cgcacgcggc tgggtgacgt cgtcgatcac ggcgtcgtcg
      961 atcgatagct gccccgcaag ctggtcgctg tctggtggcg aggcttgtgt ccctgtctgc
     1021 ccggtggagt agagggcgtg gaagccgatc cgttcatcgg cggcgacctg ctgttggtgg
     1081 gcgtcggcga ccagttgcag gtaggcgatc ccggtcgccg gcgcctgggc cggctcgggt
     1141 gtttcgggcc gcgctttcgg gtgcgcgtgg cgggtgatgc ggtgcggcac cgcgcggccg
     1201 tagtgggcgt cttggtagtg cacgtcgatg acctcgaggt tgaacgggga gaacaccagc
     1261 tcgaccttcc gcccgaccag caccggttcg acctggtagg tgttgccgtg cagcgacacc
     1321 gtcgcggtct tggtgacgac acgccattgc gaccacagga acgcctcggt caacgcgtcg
     1381 gccgaggcca tggccgggcc gtgcccggcg gcctgccagc cgtcgtccca gcgccgcaac
     1441 ggtgtctgac cggtctcgga gtgcacgctg tggtggtaga ccgattcgac ccaggcggtg
     1501 aatagggcgt tgagctcgag caacgccgca gccggtgaca atccctgctc ggcaagctgt
     1561 tcggcggtgg tgccggtgac ctcgacgagg aactggtcgc gcaccccgcg gaaccagcgt
     1621 tcgattttgc ccctgccttg cgggcggtgc ggggtggagt ggatcagccg gatgccgagc
     1681 ttgccgcacg cgcgtagcag ccacgaatcc acgtaggcac tgccgttgtc gacgtagcag
     1741 ctcgccggga cgccccgcgc cgcgagggcg ggttgcagcg cggccgccag ccgcacggtg
     1801 tcttcggcga acccgaaccg gtaccccacc gcaagacgcg aatggtcgtc caggaacgcg
     1861 aagaggtagg tcttgcgacc accgagccgt ggaccgtgca acgcgtcgcc gacccagcgt
     1921 tcgttcggcg cggcggcttc gaagcggccg aacacggccg gctcgtcgcc ggcggccgga
     1981 cccatcagtt cgcggcggtg gaacaggcgc agcagcgtcg actccgacgg cgcccacccg
     2041 gacgaggcgc gcaggatccg cgcgacctgc gcggcggtgc gctcgggatt ctcccgcttc
     2101 aacgcgaccg caagctcgag cacggtggtg tcgatgcggc taccgggctg gcgggtcgac
     2161 ggtgtgagcg cgtcgaaccc gccgacgcgg taacggcgga tccagcggtc caacgtgtcg
     2221 cgggagtagc cgtactgccc gccgaacggg ccggcatggg tgccggcggc gatcgcccgc
     2281 accacccggc cgcgggcctt ggtcgacaag cccggatcca aggcagggca gatcagctgg
     2341 tagcggaaca gcgccacctg ctgtgcccgt gcccgccggg ccgcgtcgtc gtcgtgctca
     2401 tacgtggtca cgaaacctcg attcggtcag ggaacacccg cgccgggacc ggcgcgggcc
     2461 agtcaccctc gcgaggtttc cgatcaaccg ccccgaacgt caggggcgac tcgtgttgcc
     2521 ggttgccgcc caccagccac cggccaccca ggcgcgagga gccgaccagc cgaacacgcc
     2581 acggcgacct gccacgccgt caccgcgccg agcaccccaa ccggcccgaa ccggcccgtc
     2641 accgccgcag tcgcagcacc caacgcggcc agcagatccc gccaccggca gccgagcctc
     2701 tccggcaccc tcacgtcgac accggcacgg acagcgacct gcaacagacg cacccggacc
     2761 gcttccagcc gccccgccgt cacccgtagc cagccccgca ccgttgccgc gggaacctcc
     2821 agcgccgccc cgctcgagcg gtgcccccgc ccggcggcgc gggccagcag cgccgcgccg
     2881 atcacgtcgg ccgcgtaggc ccgccgcagc agcaccgtca ccggcagcag cacatgcgtc
     2941 accaggcacc cgccacaacg accgcgacgg ggccgcaaca ccccgccgat cccgtgcacg
     3001 cggcgcggcc gggcccaacc ccacggacgc agcgcacccg aacagtcagg gcagtcgacc
     3061 cgcccgccca tcaaccgcga ctcgactcca accggatccg cctctaccgt caccaacggt
     3121 gcctccgcgc atcagcacgg ccctcccgcc acggtcctcc acgacatgac gcgggagggc
     3181 cgttgtcatc ttcgtcggca ggattcttcc catcccggca cagtgacgaa gccgaccacc
     3241 acagccgaca gcacgcagaa gatcaaaccg tcatcggcaa ccgaactgac gccaaccgcc
     3301 ccgatcgtca gcgttaagaa tgacggtcaa caaccggccc cgctccttca tggatgaccc
     3361 caacggcatc aagatccgcc tcaagcggct ggggcaggcc gccggagtat ccgacgtgca
     3421 cgcccatcgg tggcggcaca gcttcg
//
LOCUS       DASSGQ010000103        13537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1283164,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000103 DASSGQ010000000
VERSION     DASSGQ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13537
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283164"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VFI55_04705"
     CDS             complement(<1..912)
                     /locus_tag="VFI55_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073368.1"
                     /translation="MQQQQQEQYAQELHDQWTQFASRNRGHTADDLVARFQQGGKQHL
                     DLAEKYKVKAEAFERGADAIDNLREGLREIADDYNQRIANIENSNEPSPAKTADIARL
                     VAEANGFAAYKSGAAIATIMDGVQKILTVEGVPLSSGEFLNSEGLTTESSPKAGPADG
                     GPPGTHGAGGGSHGSAGIAGDGGSPGAHGAGGGSHGSAGHAGDGGSPGARGASGGSSG
                     AAGGSPPVAAQVGIGHVPGAPAISSPAPGGGGLSPAAFGGQSPASLANSFTTGMMAGQ
                     PAAADAHKFGEGILSATGSASPAQAPVV"
     gene            complement(1121..1651)
                     /locus_tag="VFI55_04710"
     CDS             complement(1121..1651)
                     /locus_tag="VFI55_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073369.1"
                     /translation="MMNPQPPPPAQWTPPAEPSRPTANGRIVLALVVIGLIAVAALVV
                     GIVDLARPNSSGASAASTSVTPSPTFTPEQIATAKKDLCSAYQLASHSVNVDTHTSDT
                     ALARISLVNGAGLLDAAAANPALRANDRNAAQALASAYRTSNAVSSDTDAASPLYQTT
                     LDDITRTNAAMAAICR"
     gene            complement(1826..3712)
                     /locus_tag="VFI55_04715"
     CDS             complement(1826..3712)
                     /locus_tag="VFI55_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7073370.1"
                     /translation="MQLRYISNPHLITEAGGDPWAINNSLQVGRPAQIADLAEAFHDA
                     GRCTAESSAAFDEARRRFEASWNRLNGVHPINDFAEVERTVQSLGAQSLQLPKIAVDL
                     ESVAVVLAEAQRTGAALISTLERQLQQTDNQLGPALDLEKDPQRNAADRTTLDALIRG
                     MEQQAIDDTKSALRQLRSLRSVYSDRLQTSLTTLRTDGYDPTVIQLVDAPETQADPQQ
                     PMQIPPPGTSAQDINRWWKTLSSEQQSRLIADHRPELGNLNGIPVDVRSRVNTAVMGD
                     DLHRVEDVAKQSGSSVDEVLGDPVRWRLTEVAITRYNNARRTQEGLTASADAVNERTN
                     DHPAVFLLKYQPEAFGGAGAAAIAMGNPDTAANTAVLVSGAGTSVREGTLAKADGARL
                     YEESARADSRHQTAVVMWVGYDTPNSWHDPGLWQPNMARTGGQALAADVNALAVTHEA
                     APTRVTVVGHSYGSTVVSDAAAAYGMRANDVVLIGSPGTDLAHSATDFHLPPGGHLYV
                     GAASGDPVTWSPGHVAGPGVFAANLAGLGDDPTGDGYGSTRFKAEVPGDWTNPFYDHL
                     HYFDDGSESLFSIADVVSGHGDALQRDGMTARHRGEYGLGGWADPEAARRATTDHRHR
                     APPR"
     gene            complement(3679..4008)
                     /locus_tag="VFI55_04720"
     CDS             complement(3679..4008)
                     /locus_tag="VFI55_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014943199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2563 family protein"
                     /protein_id="HET7073371.1"
                     /translation="MFVDTDLLHSGANESHRAGGHAQDGVEQLSRGPLLWGMFGGFPA
                     AEAFHDAVASAHAQHARNLQAQHETLTELGRKAHYAADGFTHMDDRNAAEMRAVRCSS
                     DTSATRT"
     gene            complement(4108..4617)
                     /locus_tag="VFI55_04725"
     CDS             complement(4108..4617)
                     /locus_tag="VFI55_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HET7073372.1"
                     /translation="MDYCLSGDNGAAAIWHGQPDLDLDHDGRLDAFGLDLDDDGLNDD
                     AMADFDGDGIADHAVLDIDNDGAPESYFTDDGSGTWSVAFDRGGQLRWFGLDGVEHTG
                     GPLVDFDGHGQADDRLLDTDGDGVADRVLCAGESGVTGFVDTDGDGRWNIRLTDTDGD
                     GLADGASNL"
     gene            complement(4642..6078)
                     /locus_tag="VFI55_04730"
     CDS             complement(4642..6078)
                     /locus_tag="VFI55_04730"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_178026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET7073373.1"
                     /translation="MPEADADLLTAAAVALNRHAPMLRELGSLFDAAGHELYLVGGSV
                     RDALLGRLSPDLDFTTDARPEQVQEIVRRWADAVWETGIEFGTVGVGKGEHRLEITTF
                     RADTYDQVSRNPEVRFGDRLDDDLVRRDFTVNAMAVRVTPTGSGEFLDPLGGLAALRE
                     RVLDTPAPPSVSFGDDPLRMLRAARFVSQLGFAVTPRVRGAIEEMATQLGRISAERVA
                     AELDKLLLGTDPVAGIDLLVETGMGEVVLPEVGGMRMAIDEHHQHKDVYQHSLTVLRQ
                     AIGLEDVGPDLVLRWAALLHDIGKPITRRHEPDGGVSFHHHEVVGAKMVRKRMRALKY
                     SKQMIDAVSQLVFLHLRFHGYGDGKWTDSAVRRYVTDAGPLLPRLHKLVRADCTTRNK
                     RRAARLQASYDQLEARIVELAAQEDLERIRPDLDGNQIMQLLDIPAGPEVGEAWRYLK
                     ELRLDRGPMTTEQATAELLAWWQSRGNR"
     gene            6260..6994
                     /locus_tag="VFI55_04735"
     CDS             6260..6994
                     /locus_tag="VFI55_04735"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015291814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7073374.1"
                     /translation="MSDGEQAKPRRRRGRRRGRGSAGAAENPPDDQGTGDSTPTKPRR
                     SRAARRIPERLRTVHETSAGGLVIDGIDGPRDAQVAALIGRIDRRGRMLWSLPKGHIE
                     QGETAEQTAIREVAEETGIRGSVLAALGRIDYWFVTDGRRVHKTVHHYLMRFSGGELS
                     DDDLEVAEVAWVPIRDLPSRLAYADERRLAQVADELIDKLQSDGPAALPPLPPSSPRR
                     RPQTHSRTRHSEKPATGRKNGTGPPP"
     gene            6991..9381
                     /locus_tag="VFI55_04740"
     CDS             6991..9381
                     /locus_tag="VFI55_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073375.1"
                     /translation="MTQLRICGAGLLRLAAVVGIVAGFAVLTGSVVPRAAAGEPAATT
                     FVRVRIDQVTPDVVTTTSPAVVTVTGMVTNIGDRPVRDVMVRLEHAGAITASAGLRTS
                     LDGSTDQYQPAADFFTVAPELQRGQEAGFTLSLPLRSSAKPSLGIDEPGIYPVLVNVN
                     GTPDYGIPARLDNARFLLPVVGVPPDAASDQDSAVAPDTSKPVWITMLWPLADRPRLS
                     PGVPGGAMPVRLVDDDLATSLAGGGRLDILLSAAEVATSRDVDPDGAVGRALCLAVDP
                     DLLVTVNAMTAGYVVSNSPDGPAQQPGTPTHPGTGQVAAADWLNRLRALAHRTCVVPL
                     PYAQADLDALQRVHDPGLSAIATNSVGGIVDRILDVASTRGATLLPDGPLTDGAVNLL
                     SAGDGTVAIAAADLSAQDSASDASTSVDTAPRRLSPQVVVAPFDPAVGAALAGAGTDP
                     AVPTYLDSSLSVRLGHDSATARRQDAVGSIFWHALQRDDAPRTELLVPPATWNLQSDD
                     AQVIMTALATTIRSGLAVPRPLPAVIAEAAARTDPPEPAGANGSERGQFTDAVTGDIA
                     VQVARLWGLTAALTTDERTGLTGVQYTAPLREDMLRAVSQSEPPDTRNGLAQQRLAVV
                     GKTIGDLFGAVTIVNPGGSYTLATEHSPLPLVLHNGLAVPVRVRLQVDAPPGMTVTDV
                     GQIELPPGYLPLRVPIEVKFTQRVAVDVTLRTPDGMRLGDTVRLSVHSNAYGKVLFAI
                     TMTAAAVLVTLAGRRLWHRFRGQPDRADLDRPDPPRPRRPASHHEIDHRVDHEHRV"
     gene            9378..13001
                     /gene="murJ"
                     /locus_tag="VFI55_04745"
     CDS             9378..13001
                     /gene="murJ"
                     /locus_tag="VFI55_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HET7073376.1"
                     /translation="MNDAYRRTAPEPPPRPHHPPPPPPQPRRAGGELGRRRPELSDAA
                     LVSRSWAMALATLVSRITGFARIVLLAAILGAALSSAFSVANQLPNLVAALVLEATFT
                     AIFVPVLARAEQSDPDGGTAFVRRLVTVTTTLLVFATALSVLAAPLLVRLMLGRAPQV
                     NEPLTTAFAYLLLPQVLAYGLTSVFMAILNTRNVFGPTAWAPVVNNVVGLATLAVYLA
                     VPGELSVDPVRMGNAKLLVLGIGTTLGVYAQTAVLLVALRRHRVSLRPLWGIDQRLKR
                     FGAMAFAMVLYVLISQLGLVVGYQIASTAAASGPAIYNYTWMVLMLPFGMIGVTVLTV
                     VMPRLSRNAAADDTRAVLADLSLATRLTLITLIPIVAFMTVGGPAIGSALFAYGHFGE
                     VDAGYLGAAIALSAFTLLPYGLVLLQLRVFYAREQPWTPIAIIVVITAVKIAGSVLAP
                     HLTGDPKLVAGFLGLANGVGFLAGAIVGYWLLRRALLPSGGQLIGAGEVRTILVTTVA
                     SMLAALIAHVADRLLGLGALTAHGGPASLLRLLVLAIIMLPIMAAVMVRARVPEAEAA
                     LRVVNRRLLNRGGNRNAPAAPRKQTVPDPSSQRVPVTYPEQRNSSQPGVNAVQEPIRR
                     MPSERAASARIAKGPEVTDRPVESAASGSASELPRPVADDFQPDLPPDQAWEAARAFD
                     RLNGDLSAESPRGPAPFDAPRERGGESAAGADDVHLVPGARIAGGRYRLLVFHGGAPP
                     LQFWQALDTALDRQVALTFVDPDGALPGDVLQEILSRTLRLSRIDKPGIARVLDVVRT
                     GSGGLVVAEWIRGGSLQEVADTAPSPVGAVRAMQSLAAAADAAHRAGVALSIDHPSRV
                     RVSIEGDVVLAYPATMPDANPQDDIRGIGAALYALLVNRWPLPEHGVRSGLAPAQRDA
                     TGNPVEPMVIDRDIPFQISAVAVRAVQEDGGIRSASTLLNLLQQATAVADRTEVLGPI
                     DDSPAPPTSRVPTGREYATFARRRRNVLIGVGAGVAVLVVALLVLASVVSSIFGNVGG
                     GLNKDELGLNGPSSSTASSAAPGSVAAGSMVKPTKATVFSPDGEADNPGQAGQAIDGN
                     PGTSWETDTYTDAVPFPGFKNGVGLMLQLPNPTVVGAVALDVSSTGTKVEIRSSSTPT
                     PSKLEDTTVLTPATALKPGHNTIPVRSGAATSNLLVWISTLGTTDGKSKAAISELTVQ
                     AAS"
     gene            13067..>13537
                     /gene="sigM"
                     /locus_tag="VFI55_04750"
     CDS             13067..>13537
                     /gene="sigM"
                     /locus_tag="VFI55_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigM"
                     /protein_id="HET7073377.1"
                     /translation="MGFGGNGQRERSDAELLAAHVAGDRYAFDELFHRHQRQLHRLAR
                     LTTRTPEDAEDALQDAMLSAHRGAGTFRHDAAVGSWLHRIVVNACLDRLRRAKAHPTA
                     PLEDVYPVPDRTAQVETAIVVQRALMRLPVEQRAAVVAVDMQGYSVADTAALLGI"
BASE COUNT         2100 a         4815 c         4361 g         2261 t
ORIGIN      
        1 caccaccggc gcctgggccg gcgacgccga gcccgtcgcg ctcaagatgc cctcgccaaa
       61 cttatgagca tcggctgctg ccggctgacc cgccatcatc cccgtcgtga atgaattcgc
      121 cagcgaagcc ggcgattgtc ccccgaaggc ggctggtgaa agtccaccac cgccgggtgc
      181 cggagaactt atcgcaggag cgcccggtac atgtccaata ccgacttggg cagcgaccgg
      241 agggctgcct ccggctgcgc cacttgaacc accgctagca ccgcgcgcac ccggcgaacc
      301 accatcgccc gcatggccag cacttccatg cgatccgccg ccagcaccgt gcgcacccgg
      361 cgaaccacca tcccctgcaa tgccagcact tccatgcgat ccgccgccag caccgtgcgt
      421 acccggcgga ccgccatcag ctggtccagc tttcggcgaa ctctcagtgg ttaacccctc
      481 actgttgaga aattcccccg aagacagcgg aactccctcg acggtaagga tcttttggac
      541 accgtccata attgtggcga tggccgcgcc cgacttatac gcggcgaacc cgttcgcctc
      601 ggcaactaac cttgcgatat ctgcagtctt cgcggggctg ggttcattcg aattttcgat
      661 atttgcgatt cgttggttgt agtcgtcagc aatctctcgt aatccttcgc gcaagttatc
      721 gattgcatcc gctcccctct caaaagcttc agcttttacc ttgtactttt ctgccaaatc
      781 gagatgctgt tttccgccct gttgaaagcg ggcgacgaga tcgtcggccg tatgtcccct
      841 attgcgtgaa gcgaactgtg tccactggtc atgcagttct tgcgcgtact gctcctgctg
      901 ctgctgctgc actttccaat gccccgcacc ggcccggagc atcgatgacg gctgcggcca
      961 ccaacggccg atgagtggca ccgtatattt tcccgctggc aaatcccccg gcacaggcac
     1021 cctcctgaga gctaaccccc cgttcccgcc aaattgtcgt tcagctgtcc cagcgacggc
     1081 aacccggtct acgagatcat ctgtcccttt tgttctcgtt tcatcggcaa atcgccgcca
     1141 tggcagcatt ggttcgagtt atgtcgtcaa gcgttgtctg atagagcggc gatgcagcat
     1201 ctgtatcact acttacagca ttggaggtgc gataggccga agctagcgcc tgtgcagcat
     1261 tccgatcatt agctctcagc gctggattcg ccgctgccgc atcgagcagc cccgcaccgt
     1321 taactaggga gatacgcgcg agagccgtgt ccgaggtgtg cgtatccaca ttcaccgagt
     1381 gcgacgccaa ctgatacgca gagcaaagat cctttttagc ggtcgcaatc tgctcgggcg
     1441 tgaaagtcgg cgaaggcgtc acggacgtcg aagccgcgct tgcgccgctc gaattcggtc
     1501 gcgcaaggtc gacgattccg acaacaagag cggccactgc gatcaggcca atcactacca
     1561 gtgcgagaac gatgcggcca ttcgccgtag gcctgctcgg ttcggcgggc ggtgtccact
     1621 gcgccggagg aggcggctgg ggattcatca cctcatcaag gctaatcggc tagatgcgaa
     1681 tgtcacttca ggccgccgtc ttcagaatgt ccagcaggta cgtccaagcg ccgtcgcgtt
     1741 tcagctccgc ttcgcagcga tgtggttcgg tgctatcgcg tttgaaaggc cggccaaacc
     1801 gcgcgacgaa ttagcgcgcc ggacgctagc gcggcggcgc gcgatggcgg tgatcagtcg
     1861 tcgcccttcg cgcagcttct gggtcggccc atccacccaa gccgtattcc ccgcggtgac
     1921 gtgcggtcat gccgtcgcgt tgcagcgcat caccatggcc ggagaccacg tcggcgatgc
     1981 tgaacagcga ttccgatcca tcgtcgaagt aatgaaggtg gtcatagaac ggattagtcc
     2041 agtcgcccgg cacctccgcc ttgaaccgcg tggaaccata gccgtcgccg gtggggtcgt
     2101 cgccgagtcc tgccaagttc gcggcgaaga cgccgggtcc cgccacatga ccaggtgacc
     2161 aggtgaccgg atcacccgat gccgctccta cgtagaggtg cccacctggc ggcaggtgaa
     2221 agtcggtcgc cgaatgcgcc aggtcagtcc ctggggagcc gatcagcact acatcatttg
     2281 cgcgcatccc atacgccgcg gcggcatccg aaacgacggt ggatccgtag gagtgcccaa
     2341 ccacggtcac acgtgtcggg gcagcttcgt gggtgaccgc aagggcgttc acatccgcgg
     2401 ccaacgcctg ccctccggta cgggccatgt tcggttgcca caaacccggg tcatgccagc
     2461 tgttgggggt gtcgtagccc acccacatca ccaccgcggt ttgatggcgc gagtcagcgc
     2521 gcgcggactc ctcgtacagg cgcgccccat cggctttagc cagcgttccc tcgcgcacgc
     2581 tggtgcccgc tccactaacc aaaaccgcgg tgttggccgc ggtgtcgggg ttgcccatcg
     2641 cgatggccgc cgccccagca ccgccgaaag cctcgggctg atacttcagg agaaatacgg
     2701 ccgggtgatc gttggtccgc tcgttgacgg cgtccgcgga cgccgtaagg ccttcttggg
     2761 tgcggcgggc attgttatag cgggtgatcg ctacttcggt cagcctccac ctgacaggat
     2821 ctccgagcac ctcgtcgaca gaggaaccgg attgcttggc cacgtcctcc actcgatgca
     2881 ggtcatcgcc catcacggcc gtattgaccc tgctgcggac atcgacggga atgccgttga
     2941 ggtttcccag ctccggtcgg tggtcggcga tcaatcgact ctgctgttcc gaactgagcg
     3001 ttttccacca ccgattgatg tcctgggcac tcgttcccgg tggtgggatt tgcatcggtt
     3061 gttgtgggtc cgcctgcgtt tccggagcgt ctaccagctg gatcactgtc gggtcgtacc
     3121 catcggtgcg caaggtggtc agcgacgtct gcaagcggtc cgaatagacg ctgcgcaggg
     3181 accggagttg cctgagcgct gatttagtgt catcgatggc ctgttgttcc atgccgcgga
     3241 taagcgcgtc gagcgtggtt ctgtctgccg cgttgcgttg ggggtctttc tcgagatcca
     3301 gcgccgggcc aagttggttg tcggtctgtt gtagttgacg ctccaaggtc gagatcagcg
     3361 ctgcgccggt gcgctgcgcc tcggccagga caaccgcgac gctttccaag tcgacggcga
     3421 tcttgggcaa ctgtagggac tgggcaccga gcgactggac cgtgcgctcc acttctgcga
     3481 agtcgttgat cggatgcacc ccatttagcc gattccagga agcctcaaag cgacggcgtg
     3541 cctcatcgaa cgcggcgctg gactcggcgg tgcagcggcc tgcatcgtga aacgcctccg
     3601 ccaaatcggc aatctgcgcc ggacgtccga cttgcaggct gttgttgatc gcccaggggt
     3661 caccgccggc ttcggttatc aggtgcgggt tgctgatgta tcggagctgc atcgcaccgc
     3721 ccgcatttct gcggcgttgc ggtcatccat gtgggtgaac ccgtcggcgg cataatgcgc
     3781 cttgcggccg agttcagtca gcgtctcgtg ctgggcctgc aaatttctgg cgtgttgagc
     3841 gtgcgcggag gcgaccgcgt cgtgaaatgc ctcggcggcc gggaagccac cgaacatccc
     3901 ccacagcagc ggacctcgcg atagctgctc gaccccgtct tgtgcgtgcc cgcccgcgcg
     3961 gtgcgactcg ttggcccccg agtgcagcaa gtcagtatcg acaaacatcg ctaccccctg
     4021 ccggcacgta atgcccatga actgcgacga agctaatacg ggcccgcttg ccggtcaatt
     4081 cggtcttcca ctcccccaac ggcatcctca caggttgctc gccccatcgg ccaaaccgtc
     4141 gccatcggtg tcggtcagcc tgatattcca cctgccgtcg ccgtcggtat cgacgaatcc
     4201 ggtcacgccg ctctcgccgg cgcacagcac ccggtcggcc accccgtcgc cgtcggtatc
     4261 gagcagtcgg tcgtcagcct gcccgtgacc gtcgaagtcg accagtggac cgccggtgtg
     4321 ctcaacaccg tcgagtccaa accatcgcag ctggccaccc cggtcgaacg cgaccgacca
     4381 cgtccccgat ccgtcatccg tgaagtagct ttccggtgct ccgtcattgt cgatgtccag
     4441 gacggcgtga tcggcgattc catcgccgtc gaaatcagcc atcgcgtcgt cgttcagccc
     4501 gtcatcgtcg aggtccaggc cgaacgcgtc gagccggcca tcgtggtcga gatcgaggtc
     4561 gggctgacca tgccagatgg ccgcggctcc gttatctcca gaaaggcagt agtccatgcc
     4621 tagtcggacg ccccgcctcc actagcggtt cccccgcgac tgccaccacg ccagcagctc
     4681 ggcggtggcc tgctcggtgg tcatcggacc gcgatccagc cgcagttctt tcaggtaacg
     4741 ccacgcctcg ccgacttccg gtccggcggg aatgtcgagc agctgcatga tctggttgcc
     4801 gtccaaatcg gggcggatgc gctcaagatc ctcttgggcg gccagttcga cgatgcgcgc
     4861 ctccagctgg tcatagctgg cctgtagccg cgcggcccgc cgcttgttgc gggtcgtgca
     4921 gtcggcgcgc accagcttgt gcagccgggg cagcagcggt cccgcgtccg tgacgtagcg
     4981 gcgcaccgcg gaatccgtcc acttgccgtc gccgtagccg tggaaccgca gatgcagaaa
     5041 caccagctgc gagacggcgt cgatcatctg cttggagtac ttgagcgccc gcatccgctt
     5101 gcggaccatc ttcgcgccga ccacttcgtg gtgatggaag ctcacgccgc cgtcgggctc
     5161 gtgccgccgc gtgatcggct tgccgatgtc gtgcaacagc gccgcccagc gcagcaccag
     5221 gtcggggccc acatcctcga gcccgatcgc ctggcgcagc acggtcagcg aatgctggta
     5281 gacgtctttg tgttgatggt gttcgtcgat ggccatccgc atgccgccga cctccggcag
     5341 caccacctca cccatcccgg tctccaccag caggtcgatg ccggcgaccg ggtcggtgcc
     5401 gagcaagagc ttgtccagct cggcggccac ccgttcggcg ctgatgcggc ccagctgggt
     5461 ggccatctct tcgatcgccc cccgcacccg cggagtaacg gcgaagccga gttgcgacac
     5521 gaaacgcgcg gcgcgcagca tccgcaacgg gtcgtcaccg aaggacaccg acggcggcgc
     5581 cggggtgtcg agcacccgtt cgcgcagcgc cgccaaaccg cccagcgggt caaggaattc
     5641 acccgacccc gtaggcgtta cacgcacggc catcgcgttc acggtgaaat cgcggcgcac
     5701 caaatcgtcg tcgaggcgat cgccgaagcg cacctcggga ttgcgcgaca cctggtcgta
     5761 ggtatccgcg cggaacgtgg tgatctctag gcggtgttcg cccttgccga cgccaacggt
     5821 gccgaattcg attcccgtct cccacaccgc gtcggcccat cgccgcacta tctcttggac
     5881 ctgctcgggg cgggcgtcgg tggtgaagtc caggtcggga ctcaacctgc ccagcagggc
     5941 gtcccgcacc gaaccgccga ccaggtacaa ctcgtggccc gcggcgtcga acagcgagcc
     6001 cagttcgcgc agcatgggcg cgtgccgatt cagggcgacc gcggcggcgg tcaacaggtc
     6061 ggcatcggct tcgggcacgt tcggtgagcc tagtcggccg ccgatgctgg ccggcacggc
     6121 cagaggagaa ggcggcccga caaccccgag aattggcgtt ccgcaagagg gtccctcaga
     6181 cggcgcgctg cgccgccgca caccgcgcta gcggcgagta tgtccagaag accgacctgt
     6241 gccagctact atcgcttggg tgtcggacgg cgaacaagcc aaaccacgtc ggcgccgggg
     6301 gcggcgccgc ggtcgtggct ctgccggcgc ggccgagaac ccaccggacg accaagggac
     6361 cggcgactca acaccgacca aaccgcgccg atcccgcgcc gctcgccgca taccggagcg
     6421 gctgcgcacc gtccacgaaa cctccgccgg cggcctggtc atcgacggca tcgacgggcc
     6481 gcgcgacgcg caggtcgcgg ccctcatcgg tcgcatcgat cggcgcgggc gcatgttgtg
     6541 gtcgctcccc aaaggtcaca tcgaacaagg cgaaaccgca gaacagaccg ccattcgtga
     6601 ggtcgccgag gagaccggga ttcgcggcag cgtgctggcc gcgctgggac gcatcgacta
     6661 ctggttcgtc accgacggcc gtcgcgtgca caagacggtg caccattatt taatgcgctt
     6721 ctcgggcggc gagctgtccg acgacgacct cgaagtcgcc gaggtggcct gggtgccgat
     6781 acgcgacctg ccgtcccggc tggcctacgc cgacgaacgt cggctggccc aggtcgccga
     6841 cgaactgatc gacaaactgc agagcgacgg ccccgcggcg cttccgccgc tgccgcccag
     6901 ctcgccacgc cgacgcccgc agacacattc gcggacccgg cattccgaaa agcccgccac
     6961 gggccggaag aacggtaccg ggccgccgcc gtgactcagc tgcgaatctg cggggccggc
     7021 ctgttgcgcc ttgccgcagt cgtcggcatc gtggccggtt ttgcggtgct caccggatcc
     7081 gtcgtgccac gcgcggccgc aggcgaaccc gccgcgacga cgttcgtccg cgtgcgcatc
     7141 gaccaggtga ccccggacgt ggtcaccaca acgagtccag ccgtcgtcac cgtcaccggc
     7201 atggtgacca acattggcga ccgcccggtc cgcgacgtga tggtccggct cgagcacgcc
     7261 ggggcgatca ccgcctcggc gggcttgcgc acctccctgg acggcagcac cgaccagtac
     7321 cagccggcag cggacttctt cacggtggcg cccgaacttc agcgcgggca ggaggccggc
     7381 ttcaccctgt ctctcccgct ccgctcgtcg gccaaaccat cgctcggcat cgacgagccc
     7441 gggatctacc ccgtcctggt caacgtcaac ggaacacccg actacggcat ccccgcccga
     7501 ctcgacaacg cccggttcct gctgcccgtc gtcggcgtgc cacccgacgc cgccagtgac
     7561 caggactccg ccgtcgcgcc cgacacctct aaacccgtgt ggatcaccat gctgtggccg
     7621 ctcgccgacc ggccccggtt gtctcccggc gtgcccggcg gggccatgcc ggtccgactc
     7681 gtcgacgacg acttggccac ctcgctggcc ggcggcggcc gcctggacat cctgctgtcc
     7741 gccgccgagg tcgccaccag ccgcgacgtc gacccggacg gcgccgtcgg ccgcgcgctc
     7801 tgcctggccg tcgacccgga cctgctggtc accgtcaatg cgatgaccgc cggatacgtc
     7861 gtgtccaatt cacccgacgg ccccgcacaa caaccgggca ccccgacaca cccgggcacc
     7921 ggccaggtcg ccgccgccga ctggctgaac cggctgcgcg cgctggccca ccggacctgc
     7981 gtggtgccgc tgccctacgc gcaagccgac ctggacgcgt tgcagcgcgt ccacgatccc
     8041 gggctcagcg cgatcgccac caacagcgtc ggcggcatcg tcgaccgcat tctggacgtg
     8101 gcctccaccc gcggggcaac gctgctgccg gacggcccgt tgaccgacgg cgcggtcaac
     8161 ctgctcagcg ccggcgacgg aacagtcgcc atcgccgccg cggacctgtc cgcccaagat
     8221 tcggcgagcg acgcttcgac gagcgtcgac accgcacccc ggcggttgtc gccgcaagtg
     8281 gtggtcgcgc cgttcgaccc ggccgtcggt gccgcactgg ccggagcggg caccgatccg
     8341 gcagtgccga cgtacctgga ctcctcgctg tcggttcggc ttggccacga ctcggccacc
     8401 gctcgacgcc aggacgccgt gggctccatc ttttggcacg ccctgcagcg cgacgacgcg
     8461 ccgcgaaccg agctgctggt cccgccggcg acgtggaacc tgcagagcga cgacgcgcag
     8521 gtgatcatga ccgcgctggc gactactatc cgttccggtc tggcggtgcc gcggccacta
     8581 cccgccgtga tcgccgaggc cgcggcccgc accgacccac cggagcccgc gggtgccaat
     8641 gggtcggagc gcggccagtt caccgacgcc gtcaccggtg acatcgcagt tcaagttgcc
     8701 cggctgtggg gtctgaccgc cgcgttgacc accgacgagc gcaccgggct gaccggcgtc
     8761 cagtacaccg ccccgctgcg cgaggacatg ctgcgcgcgg tgagccaatc ggaaccgccc
     8821 gatacccgca acgggctcgc gcagcagcgc ctggcggtgg tcggcaagac catcggcgac
     8881 ttgttcggcg cggtcaccat cgtcaacccc ggcggttcgt acaccctggc caccgagcac
     8941 agcccgctgc cgctggtgct gcacaacggg ctggcggtgc ccgtccgggt gcggctgcag
     9001 gtcgacgcgc cacctgggat gacggtgacc gacgtcggcc agatcgagtt gccgcccgga
     9061 tacctaccgc tgcgggtgcc gatcgaagtg aagttcaccc agcgggtcgc cgtcgacgtg
     9121 acgctgcgga cgccggacgg catgcgcctg ggcgacaccg tgcgtttgtc ggtgcattcc
     9181 aacgcgtacg gcaaggtgct gttcgccatc acgatgaccg ccgcggcggt gctggtgacg
     9241 ctggccggac gccggctgtg gcaccgtttc cgcggccagc ccgaccgcgc cgacctggac
     9301 cgtccggacc cgccccgccc gaggcggccc gcgtcgcacc acgagatcga ccaccgggtc
     9361 gatcacgagc accgagtatg aacgacgcct accggcggac cgccccggag ccgccgccgc
     9421 ggccgcacca cccgccaccg ccgccaccac agccccgtcg ggcgggcggt gagctgggtc
     9481 gacgacgacc ggaactgtcc gacgccgcgc tggtgtcccg gtcgtgggcg atggccttgg
     9541 caacattggt cagccgcatc accggcttcg cccggatcgt cttgctggcc gcgatccttg
     9601 gtgcggcgtt gtccagcgcc ttctccgtgg ccaatcagct gccgaacctg gtcgcggcgc
     9661 tcgtgctgga ggccacgttc accgcgatct tcgtgcctgt gctggcccgt gccgagcaaa
     9721 gcgaccccga cggcggcacg gcgttcgtgc gccgcctggt cacggtgacg accacgctgc
     9781 tggtgttcgc cacggcgctg tcggtgttgg ccgcgccgct gctggtgcgg ctgatgctgg
     9841 ggcgcgctcc gcaggtcaac gagccgctga ccaccgcgtt cgcctatctg ctgctgccgc
     9901 aggtgctcgc ctacggactc acgtcagtgt tcatggcgat cctgaacacc cgcaacgtgt
     9961 tcgggccgac ggcctgggcg ccggtcgtca acaacgtcgt cgggctcgcg acgctggcgg
    10021 tgtatctggc cgtccccggt gagctttccg tggaccccgt tcggatgggc aacgccaagc
    10081 tattggtgct cggcatcggc accaccttgg gtgtgtacgc gcagaccgcg gtgctgctgg
    10141 tggcgctgcg acggcaccgc gtcagcctgc gcccgctgtg gggaatcgat cagcgcctca
    10201 agcgcttcgg cgcgatggcc tttgcgatgg tgctctacgt gctgatcagc cagctcggcc
    10261 tggtggtcgg ctaccagatt gccagcaccg cggcggcttc cggccccgcg atctacaact
    10321 acacctggat ggtcctgatg ctgccgttcg gcatgatcgg ggtcaccgtg ctgacggtgg
    10381 tgatgccgcg gctgagtcgc aatgccgccg ccgacgacac ccgggccgtg ctggccgacc
    10441 tgtcgctggc cacccggctg accttgatca cgctgatccc gatagtggcg ttcatgaccg
    10501 tcggcgggcc cgcgatcggc agcgcgctgt tcgcctacgg ccatttcggg gaggtcgacg
    10561 cggggtacct gggtgccgcg atcgcgttgt cggcgttcac gttgctcccc tacgggctgg
    10621 tgctgctgca actgcgcgtg ttctacgccc gcgaacagcc ctggacgccg atcgcgatca
    10681 tcgtcgtcat cacggcagtc aagatcgccg gatcggtgct ggcgccccac ctcaccggcg
    10741 accccaagct ggtggccggt tttctcgggc tggccaacgg cgtcggattc ctggccggcg
    10801 cgatcgtcgg ttactggctg ctgcgacgcg cgctgctgcc ctccgggggg cagctgatcg
    10861 gcgccggtga ggtgcggaca attctggtga cgacggtcgc gtcgatgctg gccgcgttga
    10921 tcgcccacgt ggccgaccgg ctgctgggcc tgggcgcgct gacggcccac ggcggtccgg
    10981 cctcgttgct gcgcctgctg gtgctggcga tcatcatgct gccgatcatg gccgcggtca
    11041 tggtccgggc gcgcgtcccc gaggccgagg cggcgctccg cgtggtcaac cgccgacttc
    11101 tcaaccgcgg cgggaaccgg aacgccccgg cagcgccgcg gaaacagact gtgccggatc
    11161 catcgtctca gcgggttccc gtcacgtacc ctgagcagag gaattcgtcc cagccggggg
    11221 taaatgcggt ccaggagccg atccggcgca tgccttcgga gcgggcagcc agcgcccgga
    11281 tagcgaaagg accggaggtg acagaccgcc cagtagagag cgccgcatcc ggctccgcgt
    11341 ccgagctgcc gcggccggtt gccgacgact tccagcccga ccttccgccc gaccaggcgt
    11401 gggaagcggc gcgcgccttc gaccggctga acggcgatct ctccgctgaa tccccccgcg
    11461 gcccggcccc gttcgacgcg ccccgcgaac gcggtgggga gtcggcggct ggcgctgacg
    11521 atgtccacct ggttcccggc gcccgcatcg ccggcggccg gtatcggctg ctcgtcttcc
    11581 acggcggagc gcctcccctg cagttctggc aggcactgga caccgcgctg gaccggcagg
    11641 tggcgctgac cttcgtcgac ccggacgggg ccctgccggg cgacgtgctg caagagatcc
    11701 tctcccgcac cttgcggctc agccggatcg acaagcccgg tatcgcccgg gtgctcgacg
    11761 tggtccgcac cgggtccggc ggcctggtgg tcgccgagtg gatccgcggc ggttccctgc
    11821 aggaagtggc cgacacggcg ccgtcgcccg tcggcgccgt gcgcgcgatg cagtcgctgg
    11881 ccgcggccgc cgacgcggcc caccgcgccg gtgtcgccct gtcgatcgac caccccagcc
    11941 gggtacgcgt cagcatcgaa ggcgacgtcg tgctcgccta ccccgcgacc atgcccgacg
    12001 ccaaccccca agacgacatc cgcggcatcg gcgcggcgct gtacgccctg ctggtcaacc
    12061 gctggcccct gccggagcac ggggtgcgca gcgggctggc gccggctcag cgcgacgcca
    12121 cgggcaatcc cgtcgaaccc atggtcatcg accgtgacat ccctttccag atttccgcgg
    12181 tcgccgttcg ggcggttcaa gaggacggcg ggatacgcag tgcgtcaacg cttttgaact
    12241 tgctgcagca agccaccgcg gtggccgacc gcaccgaggt actggggccg atcgacgatt
    12301 cgccggcacc gccgacctcc cgcgtgccga cgggacggga gtacgcgacc ttcgcgcgcc
    12361 ggcgccgcaa cgtgctgatc ggtgtcggcg cgggcgtcgc cgtcctggtg gtggccttgc
    12421 tggtgctggc gtcggtggtg agctcgatct tcggcaacgt cgggggtggc ctcaacaagg
    12481 acgagctcgg gctgaacggg ccctcgtcgt cgacggcgtc gtcggcggcg cccggttccg
    12541 tcgccgcggg gagcatggtc aaacccacca aggcaaccgt cttctccccc gacggcgagg
    12601 ccgacaaccc gggccaggcc gggcaggcga tcgacggcaa ccccggcaca tcctgggaga
    12661 ccgacaccta caccgatgcc gtcccgttcc ccggcttcaa gaacggcgtc ggactgatgt
    12721 tgcagttgcc gaatcccacc gtcgtgggcg cggtcgccct ggacgtctcc agcacgggca
    12781 ccaaggttga gatccgctcg tcgtccacgc cgacgccatc caagctggaa gacaccaccg
    12841 tgctgacacc ggccaccgcg ctcaaacccg gtcacaacac catcccggtc agatccggcg
    12901 cggcgacatc gaatctgctg gtgtggatct ccacgttggg aaccaccgac ggcaagagca
    12961 aagcggccat ctcggagctg acggttcagg ccgcgtcctg acaagcgctc acagacccgc
    13021 accccggtga agtgccgcgt ttccaccgat tggttactgt ccggccgtgg gtttcggggg
    13081 aaacggccag cgggaacgca gtgacgccga gctgctcgcg gcccacgtcg ccggcgatcg
    13141 ctacgccttc gacgagttgt tccaccgtca ccaacggcag ctgcaccgcc tcgcccggct
    13201 gaccacccgc acccccgaag acgccgaaga cgcgctgcag gacgcgatgc tttccgcaca
    13261 ccgtggtgcg ggcacgtttc ggcacgacgc cgcggtcggc agctggctgc accgcatcgt
    13321 ggtcaacgcc tgcctggacc ggctgcggcg cgccaaggcc cacccgaccg cgccgctgga
    13381 ggacgtctac ccggtgcccg atcgcaccgc ccaggtcgag acggccatcg tggtgcaacg
    13441 cgccttgatg cggcttcccg tcgagcagcg ggcggcggtg gttgccgtcg acatgcaggg
    13501 ctactccgtt gccgacaccg cggcgctgct gggtatc
//
LOCUS       DASSGQ010000104        22850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1374678,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000104 DASSGQ010000000
VERSION     DASSGQ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 22850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22850
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374678"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /gene="aceA"
                     /locus_tag="VFI55_04755"
     CDS             <1..522
                     /gene="aceA"
                     /locus_tag="VFI55_04755"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HET7073378.1"
                     /translation="EGFYRVKNGLEPCIARAKAYAPYSDLIWMETGTPDLDLARKFAE
                     GVKSEFPDQMLAYNCSPSFNWRKHLDDATIARFQKELGAMGFKFQFITLAGFHALNYS
                     MFDLAYGYARNQMSAYVELQEREFAAEERGYTATKHQREVGAGYFDRIATTVDPTSST
                     TALTGSTEEGQFH"
     gene            691..1557
                     /locus_tag="VFI55_04760"
     CDS             691..1557
                     /locus_tag="VFI55_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020731747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HET7073379.1"
                     /translation="MSNATAIQRVGVVGAGQMGSGIAEVAVRAGVAVTVFEPTEALVT
                     AGRTRIVKSLERGVSAGKVTERERGRALSQLTFTTDLNDLADRQLVIEAIVEDEAVKA
                     QIFAELDRIVTDPHAVLASNTSSIPIMKIAAATKNPQRVLGLHFFNPVPVLPLVEMVC
                     TLLTDEAAAACTERFASAVLGKQVVRCSDRSGFVVNALLVPYLLSAIRMVDAGIATVE
                     DVDKAVVAGLSYPMGPLRLSDLVGLDTLKLIADKMYDEYKEPHYGPPPLLLRMVEAGR
                     LGKKSGQGFYTY"
     gene            1643..2503
                     /locus_tag="VFI55_04765"
     CDS             1643..2503
                     /locus_tag="VFI55_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane mycolic acid synthase family
                     methyltransferase"
                     /protein_id="HET7073380.1"
                     /translation="MTALRPYYEESQSIYDVSDEFFALFLDPTMGYTCAYFEREDMNL
                     EEAQNAKFDLALGKLNLEPGMTLLDIGCGWGGALVRAVEKFDVNVIGITLSRNQFEYS
                     KARLAQIPTERTVEIRLQGWEEFDDKVDRIVTIGAFEAFKLERYGAFFDRAYDVLPDD
                     GRMLLHTILAYTQKQLHERGVALTMRDIRFARFIGTVIFPGGQLPAQEDIFKFAQDAG
                     FSVERVQLLREHYARTLNIWAANLEANREKAIELQSEEIYDRYMHYLTGCEQFFDRGV
                     TNVGQFTLTK"
     gene            complement(2561..3424)
                     /gene="pcaA"
                     /locus_tag="VFI55_04770"
     CDS             complement(2561..3424)
                     /gene="pcaA"
                     /locus_tag="VFI55_04770"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane mycolic acid synthase PcaA"
                     /protein_id="HET7073381.1"
                     /translation="MAVQLTPHFGNVQAHYDLSDDFFRLFLDPTQTYSCAYFERDDMT
                     LEEAQLAKIDLSLSKLSLEPGMTLLDVGCGWGATMKRAIEKYDVNVVGLTLSENQAEH
                     VQKMFDEMDTPRSARVLLEGWEKFDEPVDRIVSIGAFEHFGRARYGRFFKMAYHVLPA
                     DGVMLLHTIARPTFKDARSRGLPLTHEIVHFTQFILAEIFPGGWLPTIPVVEEHATAA
                     GFKLARIQSLQLHYAKTLDLWAAALKANKETAIAIQSKEVYDRYMKYLTGCADLFRKG
                     YTDVDQFTLEK"
     gene            complement(3560..4582)
                     /locus_tag="VFI55_04775"
     CDS             complement(3560..4582)
                     /locus_tag="VFI55_04775"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HET7073382.1"
                     /translation="MSDVSLGSRLRSWSYALRTTNPPPDGPIDTVTRWLVVTRAAVLP
                     MTLVSGLVAALLAVGKPGVDWRWLVLAVLGITLAHTANNLMNDLYDTQVGTDSAGYPR
                     ALYAPHPVLSGLISRRTLGLAILLVNIADLAILVTLGWARGWPVVAFALAGFALSVAY
                     TAPPLRLKKRGLGEPDVFLVWGPLMVCGTYYSAVGTVGWDVVLASLPYGLLCTTVLMG
                     KHIDKIPYDAPLGIRTLPVLLGEARARTVTLAMMVGFYMLVALAVAVRAMPWPALVVV
                     LALPRLAKVWPYFRRPRPDEPPPKFPVWPLWYAALAWVHVRQAGALLVLGLAIGALLH
                     TLRFAV"
     gene            complement(4603..5304)
                     /locus_tag="VFI55_04780"
     CDS             complement(4603..5304)
                     /locus_tag="VFI55_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7073383.1"
                     /translation="MAEQIPAATVKMDGRKRRWHQHKVERRNELVDGTIEAIRRRGRY
                     LSMDEIAAEIGVSKTVLYRYFVDKNDLTTAVMMRFTQTTLIPNMAAALSSDLDGIDLT
                     REVIRVYVETVANEPEPYRFVMANGSASHSKVIADSERIIARMLALVMRRRMQMAGMD
                     TGGAEPWAFLIVGGVQLATHSWMSDPRMSRDELIDYLTMLSWNALCGIVEVGGSLEKF
                     REEPHPSPIVPPRSD"
     gene            5439..6821
                     /locus_tag="VFI55_04785"
     CDS             5439..6821
                     /locus_tag="VFI55_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HET7073384.1"
                     /translation="MVAHRASDRPEASDSPAAGLNPTAPPTGAARARASFPESFAGAD
                     PEADAQRRVGLRRMKLVALSFLIGASIVFLACRWAQAHTSVGAWVGYVGAAAEAGMVG
                     ALADWFAVTALFKYPLGLPIPHTAIIKRKKDQLGEGLGTFVRENFLSPTVIENKLRDA
                     EVAGRLGKWLSEKAHADRVASETATMLRVLVELLRDDDVQQVIDRMLVRRIAEPEWGP
                     PVGRVLASLLAENRQEALIQLLADRAFQWSLNAGEVIQRVVERDSPSWSPRFIDHLVG
                     DRIHRELMDFTDKVRRNPDHELRRSATRFLFEFADDLQHDPDTIARAEAIKGQLLARD
                     EIANAAATAWKTLKRLVLEGVDDPSSTLRTRISDTVIRVGESLRDDEALRDKVDNWIV
                     RAAQHLVSQYGVEITTIITDTIERWDAEEASRRIELYVGRDLQFIRINGTVVGALAGL
                     VIYAIAQLIP"
     gene            6909..7328
                     /locus_tag="VFI55_04790"
     CDS             6909..7328
                     /locus_tag="VFI55_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7073385.1"
                     /translation="MAPEEKLSAKVTTAASDMASDIGSFIRTQRENAHVSVRQLAERS
                     GVSNPYLSQVERGLRKPSADVLSQIAKALRVSAEVLYVRAGILEPSDKSQVRDAIITD
                     TAITERQKQILLDIYASFTQQNESIHEECPTESNSDE"
     gene            7683..8273
                     /locus_tag="VFI55_04795"
     CDS             7683..8273
                     /locus_tag="VFI55_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparin-binding hemagglutinin"
                     /protein_id="HET7073386.1"
                     /translation="MPENANIEDLRAPLLAALGAADLALATVNDLLTSIRERAGDTRA
                     ETRSRVEERRARLSKLQEDLPETLTELRERFTPEELRKAAEGYLEAATSRYNELVERG
                     EAALERLRSQLPFEDASARAEGYVDQAVELTQEALGTVASQTRAVGERAAKLVGIELP
                     KKEEAKKAPAKKATAKKAPAKKAPAKKAPAKKVTQK"
     gene            8396..8671
                     /locus_tag="VFI55_04800"
     CDS             8396..8671
                     /locus_tag="VFI55_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003917305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2516 family protein"
                     /protein_id="HET7073387.1"
                     /translation="MYTVLLILQVAVLVAAVYAFVHAALQRPDAYTAAEKLTKPVWLV
                     ILGLAVALTSILSFVFGVLGTVIAACATGVYLVDVRPKLLEIQGKSR"
     gene            8676..9134
                     /locus_tag="VFI55_04805"
     CDS             8676..9134
                     /locus_tag="VFI55_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015289074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2599 domain-containing protein"
                     /protein_id="HET7073388.1"
                     /translation="MKALLSASAAVLVAVLGWLPATAPASADPGTDTADPSAPSGPGY
                     SPPFVDHTEWTQSGGLSSLRVFPTPSARAAARRPWTAAAADEAWDEVLALSPDADTPG
                     MRAQFICHWRFAEVAQPGKTSWNLEPWRPVVDDAEMVATRCNPGGPEDTF"
     gene            9134..9823
                     /gene="deoC"
                     /locus_tag="VFI55_04810"
     CDS             9134..9823
                     /gene="deoC"
                     /locus_tag="VFI55_04810"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011742101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET7073389.1"
                     /translation="MTSRLTRSQLASLVDHTLLTPEATDADVAALVAEGAELGVYAVC
                     VSPSMVPVALEAGGGMRVAAVAGFPSGKHVSAVKAHEAAEAVAAGAGEIDMVIDVGAA
                     LAGAIDAVGRDIEAVRAAVPTAVLKVIVESAVLLSQADGRRTLAAVCRAAEGAGADFV
                     KTSTGFHPAGGASVRAVALMAETVGGRLGVKASGGIRTAADAIGMLDAGATRLGLSGT
                     RAVLDGLSRES"
     gene            complement(9836..10870)
                     /locus_tag="VFI55_04815"
     CDS             complement(9836..10870)
                     /locus_tag="VFI55_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HET7073390.1"
                     /translation="MTNPQGPPDEGPSTWARPGNQGPFGHPPTERATEQLHPGDAVQG
                     PPTVVSQGPQQTEQLSAPHAGVQRPGHQPPPTPPRGPAQKQGATNQEPAPVKSKRRFL
                     RDPLSILLVCIIVFALVIGGLIGGELYARHRADTKVAQAVACEVKDQATASFGVAPLM
                     LWQLATSHFTNISVETAGNQIRDAKGMKLAINIKDVRLASSGNSKGTIGSLDATITWT
                     ADGIKQSVQNAIPVLGPFVTNSVVTHPADGTIELKGMLDNITAKPVVSGNGLELQIVS
                     FNALGFTMPRESVQSTLDDFTANLTSNYPLGIHADSVQVTDAGVVSHFSTQNASIPTG
                     NDNDPCFADI"
     gene            complement(10867..11697)
                     /locus_tag="VFI55_04820"
     CDS             complement(10867..11697)
                     /locus_tag="VFI55_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET7073391.1"
                     /translation="MRIALAQILSGSDPAANLQLVRDYTGQAADAGAKLVVFPEATMC
                     RFGVPLAPIAEPIDGPWANEVRRIAKDSGITVIAGMFTPADDGRVANTLIAAGPGTPN
                     QPDSHYDKIHLYDAFGFAESHTVAPGHEPVVVTVEGVRVGLSICYDIRFPALYTELAR
                     RGAQLIVVSASWGAGRGKLEQWTLLARARALDSMSYIAAAGQADPGDALTGSGAPTGV
                     GGSLVASPLGEVVALAGDRPQLLVADVDVDKVSLARDSIAVLRNRRDFTQIDRAQSVG
                     "
     gene            complement(11726..12247)
                     /locus_tag="VFI55_04825"
     CDS             complement(11726..12247)
                     /locus_tag="VFI55_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HET7073392.1"
                     /translation="MPRSFELSADYGDGVEEVHRAFGEEKYWHARLADIPVDDAQVEL
                     LRVGGQSGNDGTIDVITLQIVQTKNLPALVTQLHRGDLRIRREETWGPVTNGAASASI
                     QGTIVDTPVDVSGIAELSPNAEGGARVVYRITVQVRVPFLGGKVEKLIGTHLADLVTR
                     EGRFTTEWLANNA"
     gene            12274..13401
                     /locus_tag="VFI55_04830"
     CDS             12274..13401
                     /locus_tag="VFI55_04830"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET7073393.1"
                     /translation="MKTSDAGSLFAGAHVAAAVPLAPLTTLRVGPVARRLITCTSTEQ
                     VVAVLRQLDAQVGNGDRRPILIFSGGSNLVVADTMTDLTAVRLANDAITIDGNVVRAE
                     AGAVWDDVVVKTIERGLGGLECLSGIPGSVGATPVQNVGAYGAEVSDTITRVRVLDRG
                     SHAVRWVPGSELGFGYRTSMFKRSDQSERETPSVVLEVEFALDASGRSAPLRYGELTA
                     ALNAGSGDRADPKVVREAVLMLRARKGMVLDAVDHDTWSVGSFFTNPVVPPEVYQRLA
                     GNSSNVHGPVPHYPAPDGVKLAAGWLVEQAGFGKGFPDPDPGGPEAPCRLSTKHALAL
                     TNRGGATSEDVIALARTVRDGVRDVFGVTLRPEPVLLGCRL"
     gene            13442..14803
                     /locus_tag="VFI55_04835"
     CDS             13442..14803
                     /locus_tag="VFI55_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET7073394.1"
                     /translation="MAGYGSFLTVSLFVVSTSNPPPINRRLALAALGLGVLAPGVLAA
                     CSGTAAKQAEKKETPAPQLKFQPADAATDVVPTAPISVQIADGWLQKVALTNSSGKVV
                     AGAFNQDRTVYTTTEPLGFDATYSWTGSAVGHDGKAVPVAGKFTTVSPSKKIGGGFQL
                     ADGQTVGVAAPIIIQFDAPISDKAAVEKVLTVTTSPPVEGSWAWLPDEAQGARVHYRT
                     REYYPAGATVNVDAKLYGVAFGEGAYGAQDISLHFQVGRRQVVKAEVSSHRIQVMTDA
                     GVIMDFPCSYGEADKARNVTRNGVHVVTEKYSDFYMSNPAAGYTNAHERWAVRISNNG
                     EFIHANPSSAGAQGNSNVTNGCINLSTSDAEEYYHTAMYGDPVEVTGSSIQLSYSDGD
                     IWDWAVDWDTWVAMSALPPASAHPPATQIPVTAPVTPSTAPTLSGTPTTTTTTTTSSS
                     PGG"
     gene            complement(14787..15536)
                     /locus_tag="VFI55_04840"
     CDS             complement(14787..15536)
                     /locus_tag="VFI55_04840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7073395.1"
                     /translation="MNAARGHQRVAVVTGASSGIGEAAAKKLAAQGFHVVAVARRADR
                     INALADDIGGTAIVADVTDDGAVDALAAEVGRVDVLVNNAGGARGLELVADANLEHWR
                     WMWETNVLGTLRVTRALLPKLIESGDGLIVTVTSIAALEVYDGGAGYTAAKHAQGALH
                     RTLRGELLGKPVRLTEIAPGAVETEFSLVRFDGDQQRAGAVYTGITPLVAADVAEVIA
                     FVASRPSHVNLDLVVMRPRDQASATRFNRRG"
     gene            15709..17031
                     /locus_tag="VFI55_04845"
     CDS             15709..17031
                     /locus_tag="VFI55_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET7073396.1"
                     /translation="MRSTSLTHSHRSPIRKSHLRVHRQILPPSLHISDSAAASVFRAV
                     RLRGPVGRDVIAAVTSLSIATVNRQVIALLDAGLLRERADLAVSGAIGRPRVPVEINH
                     EPFVTLGIHIGARTTSIVATDLFGRTLDTVETPTPLSPAGPALASLAESASRYLRRWH
                     RRRPLWVGVAIGGTVDSNTGHVDHPRLGWRQAPVGPVLADALGLPVSVASHVDAMAGA
                     ELLLGLRRFAPSSPTSLYVYARETVGYALVIGGRVHCPASGPGTIAPLPAYSELLGGS
                     GQLESTVSDEAVLAAARRLKILPSVPPAAPATRTNGSTSAMTDLLRVARAGNQQARDL
                     LAERGRVLGEAVALLRDLLNPDELVVGGQAFTEYPEAMEQVEATFAARSILAPRDIRV
                     TAFGNRVQEAGAGTVSLGGLYADPLGSMRRAGVLDSGLRDVAADESSA"
     gene            17092..18429
                     /gene="mshA"
                     /locus_tag="VFI55_04850"
     CDS             17092..18429
                     /gene="mshA"
                     /locus_tag="VFI55_04850"
                     /EC_number="2.4.1.250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011742109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="HET7073397.1"
                     /translation="MRHDDVFRLKDPRRVAVLAVHTSPLAQPGIGDAGGMNVYVLQTA
                     LHLARRGIEVEIFTRATASADPPVARVAPGVLVRNVVAGPFEGLDKYDLPTQLCAFAA
                     GVLRAEASHEPGYYDIVHSHYWLSGQVGWLARDRWAVPLVHTAHTLAAVKNAALADGD
                     APEPPLRTVGEQQVVDEADRLIVNTEDEAKQLISIHHADPARIDVVHPGVDLDVFRPG
                     DRRAARAALGLPLDEDVVAFVGRIQPLKAPDIVLGAAAKLPGVRIVVAGGPSGSGLAS
                     PDGLLRLADELGITDRVTFLPPQSRTDLATLFQAADLVAVPSYSESFGLVAIEAQASG
                     TPVVAAAVGGLPVAVRDGISGTLVSGHDVGDWAAALDRLLRLGAPQADGMSRAAVAHA
                     ANFSWENTTDALLASYRRAIGDFTARQRRGAVKPDRASMRKPRRWTSRRGVDA"
     gene            18512..19042
                     /locus_tag="VFI55_04855"
     CDS             18512..19042
                     /locus_tag="VFI55_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HET7073398.1"
                     /translation="MGATVQDVIEHTLQASELTYSKHEGAHGGPPGLVVELPGERKLK
                     TNTILSIGEHSVRVEAFVCRKPDENHEGVYRFLLKRNRRLYGVAYTLDNVGDIYLVGR
                     MSLASVSPEEIDRVLGQVLEAVDSDFNTLLELGFRSSIQKEWEWRVSRGESLKNLEAF
                     AHLIDDEDDDAERGAR"
     gene            19232..19981
                     /locus_tag="VFI55_04860"
     CDS             19232..19981
                     /locus_tag="VFI55_04860"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HET7073399.1"
                     /translation="MGDTATLVLLRHGESDWNALNLFTGWVDVGLTEKGRAEAVRSGE
                     LLTQHDLLPDVLYTSLLRRAISTAHLALDTADRLWIPVRRTWRLNERHYGALQGLNKA
                     ETKERYGEEQFMAWRRSYDTPPPPIEQGSEFSQDADPRYANIGGGPLTECLADVVVRF
                     LPYFTDVIVPDLRTGKTVLIVAHGNSLRALVKHLDQMSDDDIVGLNIPTGVPLRYDLD
                     ADMRPLLPGGTYLDPEAAAAGAAAVAGQGRG"
     gene            20132..21352
                     /locus_tag="VFI55_04865"
     CDS             20132..21352
                     /locus_tag="VFI55_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7073400.1"
                     /translation="MTMFSALLLAGVLSVLALAVGVAVGTRLSPRVVQRRQHVSTEWT
                     GITVAQMLQRIVALMPLGAAVVDSHRDVVYLNDRAKELGLVRDRQLDDQAWQAAQQAL
                     NGVDTEFDLRPGKRAAGRSGLSVHGQARLLSEEDRRFAVVFVHDQSDYARMEATRRDF
                     VANVSHELKTPVGAMALLAEALLASADDPETVRRFAEKVLIEANRLGDMVAELIELSR
                     LQGAERLPNVTDVEVDIVVSEAISRHKVAADNAHIEVRTDAPSGLRVLGDETLLVTAL
                     ANLVSNAIAYSPPGSPVSISRRRRGDNIEIAVTDRGIGIALEDQERVFERFFRGDKAR
                     SRATGGSGLGLAIVKHVAANHSGSIGVWSKPGTGSTFTLSIPAYQDSDEAPEQPRGRE
                     VRPNRSQREEELSR"
     gene            21378..22061
                     /gene="regX"
                     /locus_tag="VFI55_04870"
     CDS             21378..22061
                     /gene="regX"
                     /locus_tag="VFI55_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensory transduction protein RegX"
                     /protein_id="HET7073401.1"
                     /translation="MTSVLIVEDEESLADPLAFLLRKEGFEATVVTDGPAALAEFDRA
                     GADIVLLDLMLPGMSGTDVCKQLRARSSVPVIMVTARDSEIDKVVGLELGADDYVTKP
                     YSARELIARIRAVLRRGGDDDSEISDGVLESGPVRMDVERHVVSVNGDTITLPLKEFD
                     LLEYLMRNSGRVLTRGQLIDRVWGADYVGDTKTLDVHVKRLRSKIEADPANPVHLVTV
                     RGLGYKLEG"
     gene            complement(22068..22565)
                     /locus_tag="VFI55_04875"
     CDS             complement(22068..22565)
                     /locus_tag="VFI55_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7073402.1"
                     /translation="MPVPDFIVELRRAIGDAPLWLAGVTAVTIHDRKVLLVKRSDNGA
                     WTAVTGIVEPGENPADCAAREVSEETGVRAQATRLAWVHVTRPTVHANGDQAQYLDLV
                     FRMRWLSGEPFAADDESTAAQWFDLDTLPPMTEDMRRRITLGASDDARTAFDTNGPLP
                     AEPSR"
     gene            complement(22631..>22850)
                     /locus_tag="VFI55_04880"
     CDS             complement(22631..>22850)
                     /locus_tag="VFI55_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HET7073403.1"
                     /translation="LAAFHPTGTAAAGADEQLCPVDAAGRLRGAEGVWVADASILPSC
                     PEVNPQLSIMALALGVADEIVDQAAGAS"
BASE COUNT         3881 a         7380 c         7730 g         3859 t
ORIGIN      
        1 gaaggcttct accgggtgaa gaacggcctc gagccatgca tcgcccgtgc caaggcctac
       61 gcgccgtact cggatctgat ctggatggag accggcacgc cggacctgga ccttgcccgc
      121 aagttcgccg agggcgtcaa gagcgagttc cccgaccaga tgctggccta caactgctcg
      181 ccgtcgttca actggcgcaa gcacctcgac gacgcgacga tcgcccggtt ccagaaggag
      241 ctcggcgcga tgggcttcaa gttccagttc atcacgctgg ccggcttcca cgcactgaac
      301 tactcgatgt tcgatctggc ctacggttac gcccgcaacc agatgagcgc ctatgtcgag
      361 ttgcaggagc gcgagttcgc cgccgaggag cgtggctaca ccgccaccaa gcaccagcgc
      421 gaggtgggtg ccggttactt cgaccgcatc gccacgacgg ttgacccgac ctcgtcgacc
      481 accgcgctga ccggctccac cgaagagggt cagtttcact gagccactga gccactgagg
      541 atgagccctt gccgaggaga cgcaaaatcg cacgtttgcg gttgaaatga tgcgattttg
      601 cgtctgctcg aaaccaacaa taggtcccgc ccacccaacc tggacgggac ctattgcgtt
      661 gcaggacaat atgactggag aaggcgacaa gtgagcaacg caacggcgat ccagcgggta
      721 ggggttgtcg gggctgggca gatgggatcc ggcatcgccg aggtcgccgt ccgcgccggt
      781 gtcgccgtca cggtcttcga gcccaccgag gcgctcgtca cggcgggacg cacccgtatc
      841 gtgaagtcgt tggaacgcgg cgtcagcgcg ggcaaggtga ccgagcggga gcgtggccgc
      901 gccctcagcc agctgacgtt caccaccgac ctgaacgact tagccgaccg gcaactggtg
      961 atcgaggcca tcgtcgaaga cgaggccgtg aaggcccaga ttttcgccga actcgaccgg
     1021 atcgtcaccg atcctcacgc ggtgctggcg tccaacacgt ccagcatccc gatcatgaag
     1081 atcgccgcgg ccaccaagaa tccacagcgc gtgcttggcc tgcacttctt caatccggtc
     1141 ccggtgctgc cgctggtcga aatggtctgc acgctgctca ccgacgaagc tgccgccgct
     1201 tgcaccgagc gatttgccag tgcggtcctg ggcaagcagg tggtgcgctg ctccgaccgg
     1261 tcgggcttcg tggtgaacgc gctgttggtg ccgtatctgc tatcggcgat ccggatggtc
     1321 gacgcgggca ttgccaccgt cgaggacgtc gacaaagccg tcgtcgccgg gctgtcgtac
     1381 ccaatgggcc cgctgcggtt gtcagacctc gttggcctgg acaccctcaa actgatcgcg
     1441 gacaagatgt acgacgaata caaagaaccg cattatggtc cgccgccgct gttgttgcgg
     1501 atggtggagg cgggtcggtt ggggaaaaag tcaggtcagg gtttctatac gtactgaggc
     1561 cgagtggtga gttggagtag cagccgttgc tcaaatcaga tatcatgctc gggttaaacg
     1621 agcgagaggt attgggcaag caatgactgc gttgaggccg tattacgaag agtcgcaatc
     1681 aatctatgac gtttcagatg aatttttcgc attatttcta gacccgacta tgggctacac
     1741 gtgcgcttac tttgagcgtg aagatatgaa cctcgaggaa gcgcaaaacg ccaagtttga
     1801 tctggcgctg ggaaagctga accttgagcc ggggatgacg ttgctcgaca ttggctgcgg
     1861 ctggggtgga gcgctggtgc gcgcggtcga gaagttcgac gtcaatgtca tcggaattac
     1921 gctcagccgc aatcagttcg agtacagcaa agccaggttg gcccaaatcc ccaccgagcg
     1981 caccgtcgag atccggctgc agggctggga agagttcgac gacaaggtcg accgaatcgt
     2041 caccatcggc gccttcgaag cgttcaagct ggagcgttat ggggccttct tcgatcgcgc
     2101 ctacgacgtt cttcccgatg acggcaggat gttgctgcac acaattttgg cgtacaccca
     2161 gaagcaactg cacgaaaggg gcgtcgcgct gacgatgcgc gacatccgat tcgcgcgttt
     2221 cattggcacc gttattttcc cgggcggaca gttgccggcg caggaagaca tattcaagtt
     2281 cgcgcaggac gccgggttct cggtcgaaag ggtgcaattg ctgcgggagc attacgcgcg
     2341 gacgctgaac atttgggcgg caaatctgga agccaaccga gaaaaggcga tagagctgca
     2401 gtcagaagag atctacgacc ggtacatgca ctacttgacc ggatgtgagc aattcttcga
     2461 caggggcgta accaacgtcg ggcagttcac gctgacgaag tagcccgcgg ccgtcgtacg
     2521 cggcccgtgc gcggatgcgc gcacgggctc acggcggcag ttatttttcc agggtgaact
     2581 ggtcgacgtc ggtgtagccc ttgcggaaca ggtccgcgca acccgtgagg tacttcatgt
     2641 accggtcgta gacctctttg gactggatgg cgatcgccgt ttccttgttt gccttgaggg
     2701 cggcggccca cagatccagg gttttggcgt agtgcagctg cagcgactgg atacgggcca
     2761 gtttgaaacc cgccgcggtt gcgtgctctt caaccaccgg gatggtcgga agccagccgc
     2821 cggggaagat ctcggccagg atgaattggg tgaagtgcac gatttcgtgg gtcagcggca
     2881 gcccacgcga cctggcgtcc ttgaaagtgg ggcgcgcgat ggtgtggagc agcatgactc
     2941 cgtctgccgg caacacgtgg taggccatct tgaagaagcg gccatagcgc gcgcggccga
     3001 agtgctcgaa ggcgccgatc gagacgatgc ggtcgaccgg ctcgtcgaac ttctcccagc
     3061 cctccagtag cacccgcgca gagcgcgggg tgtccatctc gtcgaacatc ttctggacgt
     3121 gctcggcctg gttctcggac agcgtcaggc ccacgacgtt gacgtcgtat ttctcgatgg
     3181 cgcgcttcat ggtcgcgccc cacccgcagc cgacgtccag cagcgtcatc ccaggctcca
     3241 ggctcaattt cgacagcgac aggtcgatct tggcgagctg tgcctcctcg agcgtcatgt
     3301 cgtcacgctc gaagtaggcg cagctgtacg tctgggtcgg atccaggaac agccggaaga
     3361 agtcgtcgga cagatcgtaa tgggcttgaa cattcccaaa atgtggcgtg agctgcacag
     3421 ccataaggaa gaaagccttt ctgtatatcg gggttcggga cgcattggca ggcctcgcac
     3481 cccaggtcta cccccgatgc attccatgca tgttagccgt taacgcggtt gaagtcgcat
     3541 tgcgcagcgg cgcttcgcgt cacacagcga atcgcaaggt gtgcaatagg gcgccgatcg
     3601 cgaggcctaa caccagcagc gcacccgcct gacgcacgtg cacccaggcc agcgccgcat
     3661 accacaatgg ccacaccgga aactttggtg gcggctcgtc gggcctcgga cggcggaagt
     3721 acggccacac cttcgccagc ctgggcaggg ccaggacgac gaccagcgcc ggccagggca
     3781 tcgcccgcac cgcgactgcg agcgccacca gcatatagaa gccgaccatc atcgccagcg
     3841 tcaccgtccg ggcgcgcgcc tcacccagca ggacgggcag cgtccggatc ccgagcggtg
     3901 cgtcgtacgg gatcttgtcg atgtgcttgc ccatcagcac cgtcgtgcac aacaagccat
     3961 agggcagcga tgccagcacg acgtcccagc ccacggtgcc caccgcggag tagtacgtgc
     4021 cgcataccat cagcggaccc cacacgagga agacgtcggg ctcacccagg ccgcgcttct
     4081 tcagtcgcag cggtggggcg gtgtaggcga cgctcagcgc gaacccggcg agcgcaaagg
     4141 ccaccacggg ccagccgcgt gcccacccca aggtgaccag gatcgccaga tcggcgatgt
     4201 tcaccagcag aatcgccagg cccagcgtgc ggcggctgat cagcccggag agcaccgggt
     4261 ggggtgcgta aagcgcgcgc gggtagccgg cgctgtcggt gccgacctgg gtgtcgtaca
     4321 ggtcgttcat caggttgttg gcggtgtgcg ccagcgtgat ccccagtacg gccaagacca
     4381 gccagcgcca gtcgaccccg ggcttaccga ccgccagcag cgccgcgacc aggccggaga
     4441 ccaaggtcat cggcagcacc gcagcgcggg tgactacgag ccaccgcgtg acggtgtcga
     4501 tcggcccgtc cggcggcggg ttggtggtgc gaagtgcgta cgaccatgac ctcagccgtg
     4561 agcccaggct cacgtcagac attcctaaag cctaggccga cctcaatccg accgcggagg
     4621 aacgatggga gatggatgcg gctcctcgcg gaacttctcc agcgatccgc cgacctcgac
     4681 gatcccgcac agcgcgttcc aactgagcat ggtcaagtag tcgatgagct cgtctctgct
     4741 catccgggga tccgacatcc acgagtgggt ggccagttgc acgccgccga cgatcaggaa
     4801 agcccagggt tccgctccgc cggtgtccat ccccgccatc tgcatgcgcc ggcgcatcac
     4861 cagcgcgagc atgcgggcga tgattcgctc ggagtcggcg atcactttgc tgtggctggc
     4921 tgagccgttc gccatcacga agcggtacgg ctcaggttcg ttcgccacgg tttcgacgta
     4981 cacccggatg acctcgcggg tcaggtcgat gccatccaga tccgacgaca gtgccgcggc
     5041 catgttggga atcaatgtgg tttgggtgaa gcgcatcatc accgcggtgg tgaggtcgtt
     5101 cttgtcgacg aagtagcggt acagcacggt cttggagacg ccgatttcgg ccgcgatctc
     5161 gtccatgctc aggtagcgtc cgcgccggcg aatcgcctca attgtgccgt caaccagctc
     5221 attgcggcgc tcaaccttgt gttggtgcca gcgccgtttg cgaccatcca tcttcacggt
     5281 cgcagccggt atttgctctg ccactttcgc tgcttcccac tcatcagacg cccccgatac
     5341 tacggcttca gggggtcctt ttcgcgcgcg gccgcgcagc cgcgggggcc ctagataccg
     5401 ggccgtcaca tcaactcccc gcggcaatag atgatggtgt ggtggcacac agagcctcag
     5461 acaggccaga agcgtccgac tcgccggcgg cggggttgaa cccgaccgct ccgccgaccg
     5521 gtgctgcccg ggctcgcgcc tccttcccgg aatcgttcgc cggggccgat ccggaggccg
     5581 atgcccagcg gcgggtgggg ttgcgcagga tgaagctggt cgcgttgagc tttttgatcg
     5641 gtgccagcat cgtgtttctg gcgtgccgct gggcgcaggc ccacaccagc gtgggggcgt
     5701 gggtcggcta cgtcggtgcg gccgccgaag ccggcatggt gggcgccctg gccgactggt
     5761 ttgcggtgac cgcactgttc aagtacccgc tgggcttacc catcccgcac accgcgatca
     5821 tcaagcgcaa gaaggaccag ctcggcgagg gcctgggaac cttcgtgcga gagaacttcc
     5881 tgtcgccgac ggtgatcgag aacaagctgc gcgacgccga ggtggccggc cggctcggca
     5941 agtggctgtc ggagaaggcg cacgcagacc gggttgccag cgagacggcg acgatgctgc
     6001 gggtgctggt ggagctgctg cgcgacgacg acgtccagca ggtgatcgat cggatgctcg
     6061 tgcgccgcat cgccgaaccg gagtggggtc cgccggtggg ccgggtgctg gcgagcctgc
     6121 tggccgagaa ccggcaggag gccttgatcc agttgctggc cgaccgggcg tttcagtggt
     6181 cgctgaacgc cggcgaggtc atccagcggg tggtcgagcg tgactcgccg agctggtcgc
     6241 cccggttcat cgaccaccta gtcggcgacc gcatccaccg cgagctgatg gacttcaccg
     6301 acaaggtgcg gcgcaacccc gaccacgaat tgcgccgttc ggcgacccgc ttccttttcg
     6361 agttcgccga cgacctgcag cacgacccgg acaccatcgc gcgcgccgag gcgatcaagg
     6421 ggcagctgct ggcgcgcgac gagatcgcca atgccgcggc gacggcatgg aaaacgctga
     6481 aaaggttggt gctcgagggc gtcgacgacc cgtccagcac gttgcggaca cgcatttccg
     6541 acaccgtgat ccgggtaggg gagtcactgc gcgacgacga agccctgcgc gacaaggtcg
     6601 acaactggat tgtgcgggcg gcccagcatc tggtgtccca gtacggggtt gaaatcacca
     6661 cgatcatcac cgacacgatc gagcgctggg acgccgagga ggctagtcgg cgaatcgaac
     6721 tgtacgtggg tcgtgacctg caattcattc gcatcaatgg caccgtggtg ggtgcattgg
     6781 cggggctggt gatctatgcg atcgcgcagt taattcccta ggggtgctaa caagtgcttg
     6841 caaaagcaag caccattccg tagactggaa ttcgtcaaga tctgccgtcg gattcaggga
     6901 gtaacgaaat ggcaccggag gagaaactct ccgccaaggt gaccacggcg gcgtctgaca
     6961 tggcttctga catcggcagc ttcatcagaa cccagcgtga aaatgctcac gtttcggtgc
     7021 gacaacttgc cgaacgatcc ggtgtcagta acccttacct gagccaggtg gagcgcggat
     7081 tgcgcaaacc ttccgccgat gtcctgagcc aaatcgcgaa ggcgctgcgg gtttccgccg
     7141 aggttctgta tgtacgggcc gggattctcg agcccagcga caagagtcaa gtgcgcgacg
     7201 cgatcatcac cgatacggca attaccgagc ggcagaagca aattctgctc gacatctacg
     7261 cgtcgttcac ccaacaaaac gaatccatcc acgaggagtg tccgaccgaa tccaacagcg
     7321 acgagtgatg tgacccgtcg tggccgatac tgttacgcag tcggccgggc accggagggc
     7381 cggcaaggct tcgcatcgcg tcagtgagtg acgtatatgc cgccgaccac ttggttaccc
     7441 aagaggaggg gcctaacgtc agtcgcgcga acaatgcgag ctcgctaaat caaccgacca
     7501 aatccacacg accaatcagg aggttctttc gcaagctgtg cgccgtgtca attcgcaggt
     7561 cttgactact cctccagatc ctcccgatcc tccggatctt cccgaggagg cgacgcttgc
     7621 ccctctacca gaccacccac gcagtaacgc cgataacgaa atcaaacatg aaaggaagca
     7681 caatgcctga aaacgcaaac atcgaagacc tgcgggctcc gttgctggca gccctgggcg
     7741 cggctgacct ggccctggcc acggtcaacg acttgctcac cagcatccgt gagcgcgccg
     7801 gggacacccg cgccgagacc cgcagccggg tcgaggagag gcgtgctcgc ctgtccaagc
     7861 tgcaggagga cctgcccgag acgctgaccg agctgcgcga gaggttcacc cccgaggaac
     7921 tgcgcaaggc cgccgagggc tacctggagg ccgcgaccag ccggtacaac gagctggttg
     7981 agcgtggcga ggccgccctg gagcggctgc gcagccagct gcccttcgag gacgcgtcgg
     8041 cgcgcgccga gggctacgtg gaccaggctg tcgaactgac gcaggaggcg ctgggcacgg
     8101 tcgcctcgca gacccgtgcc gtcggtgagc gcgcggccaa gctggtcggc attgagctgc
     8161 cgaagaagga agaggccaag aaggctccgg ccaagaaggc gactgccaag aaggctccgg
     8221 ccaagaaggc tccggccaag aaggctccgg ccaagaaggt cacccagaag tagtcagtga
     8281 ccgagtcgcc cccgggcgac ccggacatcg caagacactc gactccgagt cgccattggg
     8341 cgactcggag tcgacgtttt ggacgctccc cgcatacgct tagtgcgtga gcctcgtgta
     8401 tacagtcctg ttgatcttgc aggtcgccgt cttggtggcg gccgtgtacg cgtttgtgca
     8461 cgcagcgctg cagcggcccg acgcctatac cgccgctgag aagctgacca agccggtgtg
     8521 gctggtgatc ctcggcctgg ccgtcgcgct gacatctatc ctgagcttcg ttttcggcgt
     8581 cctgggcacg gtgatcgcgg cctgcgccac aggcgtgtat ctggtcgacg tgcggcccaa
     8641 gctgctcgaa attcagggta agtcgcgcta gcgggatgaa agccctgctg tccgcgtcgg
     8701 cagcggtact tgttgccgtg ctggggtggc taccggcgac ggcgcccgcg tcggccgatc
     8761 ccggcaccga tactgccgac cccagtgccc cgtccggccc cggctattcc ccgccctttg
     8821 tcgatcacac cgaatggacc caatctggag gcctgagcag ccttcgggtg ttcccgaccc
     8881 cgtctgcgcg cgcggccgcc cggcgaccct ggacggctgc cgccgccgac gaggcgtggg
     8941 acgaggtgct cgcgctgtcg ccggacgccg acaccccggg tatgcgggcg cagttcatct
     9001 gccactggcg gttcgccgaa gtggcgcaac cgggcaagac cagctggaac ctcgagccgt
     9061 ggcggcccgt ggtcgacgac gccgaaatgg tcgccacccg ctgcaatccc ggtggcccag
     9121 aagacacctt ctagtgacga gccgactcac ccggtcgcag ttggcctcgc tggtcgacca
     9181 caccctgctc acacccgagg ccaccgatgc tgatgtggcc gccctggtcg ccgagggcgc
     9241 cgagttgggc gtatatgcgg tgtgcgtctc gccgtcgatg gtgccggtgg cattggaggc
     9301 gggcgggggt atgcgcgtgg cggcggtggc gggtttcccg tcgggcaagc atgtctcggc
     9361 ggtcaaggcg cacgaggccg cggaggcggt ggcggccggg gccggcgaga tcgacatggt
     9421 catcgatgtc ggtgccgcgc tggccggggc gatcgatgcg gtgggacgcg atatcgaggc
     9481 ggtgcgggct gctgtaccga cagccgtgct caaagtcatc gtggagtcgg cggtcctgtt
     9541 gagccaggcg gacggccgcc gcacgctggc agcggtgtgt cgtgccgccg agggcgcggg
     9601 tgcggatttc gtcaagactt cgaccgggtt tcaccctgcc ggcggggcgt cggtgcgtgc
     9661 ggtcgccctg atggccgaga ccgtcggcgg gcggctgggc gtcaaggcca gcggcggaat
     9721 ccgcaccgcc gccgacgcga tcgggatgct ggatgccggc gcgacgaggc tgggcctgtc
     9781 aggcacgcgg gcggtgctcg acgggctcag ccgggaaagc tagagcgccc cgacgtcaga
     9841 tgtcggcgaa gcaggggtcg ttgtcgttgc cggtcgggat gctggcgttc tgcgtcgaga
     9901 agtggctcac gacgccggca tcggtgacct gcacactgtc ggcgtgaata cccaacgggt
     9961 agttgctggt gaggttcgcc gtgaagtcgt cgagcgtcga ctgcactgac tcccggggca
    10021 tcgtgaaccc gagcgcgttg aaactgacga tctgcagttc caacccgttg ccggacacca
    10081 ccggcttggc cgtgatgttg tccagcatgc ccttcaattc gatggtgccg tcggcgggat
    10141 gcgtgaccac gctgttggtg acgaatgggc ccagaactgg aatcgcgttt tgcaccgact
    10201 gcttgatgcc gtcggcggtc caggtgatgg tggcatcgag cgagccgatc gtcccctttg
    10261 agttaccgct cgacgcgaga cggacgtctt tgatgttgat ggcgagcttc atgcccttgg
    10321 cgtcgcggat ctggttgccg gccgtctcca ccgagatgtt ggtgaaatga ctggtggcga
    10381 gctgccagag catcaggggt gccacgccga acgacgcggt ggcctgatcc ttgacctcgc
    10441 atgcaaccgc ttgcgcgacc ttggtgtccg cccgatgacg ggcgtacagc tcgccgccga
    10501 tgagcccgcc gatgacgagc gcgaacacga tgatgcagac cagaaggatg gacagcgggt
    10561 cgcgaaggaa gcgtcgtttg ctcttcaccg gtgccggttc ttggttcgtg gcgccctgct
    10621 tttgcgcggg accccggggt ggggtcggcg gaggctggtg gccgggtcgc tgcacgcctg
    10681 cgtggggcgc gctgagctgt tcggtctgct gcggcccttg actgactacg gtcgggggcc
    10741 cttggactgc atcgccgggg tgcagctgct ccgtggcccg ctcggtgggc gggtggccga
    10801 agggaccctg gttaccggga cgggcccacg tcgacgggcc ctcgtcgggt ggtccttgtg
    10861 ggttcgtcac ccgaccgatt gtgccctatc aatctgagtg aagtcgcggc ggttgcgcag
    10921 cactgcgatg ctgtcccgag ccagggacac cttgtcgacg tcgacgtcgg cgacgagtaa
    10981 ttgcggccgg tcacccgcca aggccaccac ttcgcccagc ggtgaggcca ccaggctgcc
    11041 acccactcca gtcggcgcgc ccgagccggt cagggcgtca ccgggatcgg cctgaccagc
    11101 cgcggcgatg tagctcatcg agtccagcgc ccgggcccgg gccagcagcg tccactgctc
    11161 gagtttcccg cgccccgcac cccacgacgc actgacgacg atcagctgag cgccgcggcg
    11221 ggccagctcg gtgtagagcg ccggaaaccg aatgtcatag cagatgctta accccacccg
    11281 aacgccctcg accgtgacca ccaccggctc atgcccgggc gcgacggtgt gggactcggc
    11341 aaagccgaac gcgtcataga ggtggatctt gtcgtagtgg gaatccggct ggttgggagt
    11401 gccaggcccg gccgcgatca gcgtgtttgc cactcgcccg tcgtcggcgg gagtgaacat
    11461 gccggcgatc accgtgatgc cggagtcttt cgctatccgg cggacttcgt ttgcccaggg
    11521 cccgtcgatc ggctcggcga tcggtgccaa cgggacgccg aatcggcaca tcgtcgcttc
    11581 cgggaagacc accagcttcg cgcccgcgtc ggccgcctgg ccggtgtagt cgcgcaccag
    11641 ctgcaaattc gctgccggat cgctgccgct gaggatttgc gccaaagcga ttcgcatggc
    11701 cgccagctta ggctcggcgc tccggctagg cgttattggc gagccactcc gtcgtgaagc
    11761 gtccttctct ggtgaccagg tcggccaagt gcgtgccgat cagcttctcc accttgccgc
    11821 cgaggaaagg gacccgcacc tgcacggtga tccggtacac gacccgggcc ccgccttcgg
    11881 cattcgggga cagttcggcg atacccgaca catccaccgg agtgtcgaca atcgtgccct
    11941 ggatggaggc cgacgcggcg ccattggtga ccggccccca tgtctcctcg cgcctgatgc
    12001 ggagatcccc gcggtgcagc tgcgtgacga gggcgggcag gtttttggtt tgcacgatct
    12061 gcagcgtgat cacgtcgatg gtgccgtcgt ttccggactg accgccgacc cgcagcaact
    12121 ccacctgtgc gtcgtcgacg ggaatgtcgg ccagtctggc atgccagtac ttctcttcac
    12181 cgaatgctcg gtggacctct tcaacgccgt ccccgtaatc ggccgacaac tcaaatgaac
    12241 gcggcatagc tggccaggct accgttacgg gccatgaaaa cgagcgatgc cggttcgctc
    12301 ttcgccggtg cgcatgtcgc cgcggcggtg ccgctcgcgc cgttgaccac cttgcgggtc
    12361 gggccagtgg cacgacggct gatcacgtgc accagcaccg agcaggtggt cgccgttctg
    12421 cggcagctgg atgcgcaggt cggcaacggg gaccgtcgac cgatcctgat cttttccggc
    12481 ggctccaact tggtggtggc cgacaccatg accgatctga ccgcggtgcg attggccaac
    12541 gacgccatca ccatcgacgg caacgtggtg cgcgcagagg cgggcgcggt gtgggacgac
    12601 gtggtggtca agaccatcga acgcgggctg ggcgggctgg aatgtctgtc cggtatcccc
    12661 gggtcggtcg gcgcgacccc ggtgcagaac gtcggggcct acggggcaga agtgtccgac
    12721 accatcacgc gggtccgggt gctggaccgc ggcagccacg cggtgcgctg ggtacccggc
    12781 agcgaactgg gcttcggcta ccgcaccagc atgttcaagc ggtccgacca gtcagagcgt
    12841 gagacccctt ctgtcgtgct ggaggtggag tttgcgctgg acgcctcggg tcgtagcgca
    12901 ccgctgcgct acggagaatt gacggccgca ttgaatgccg gtagcggcga tcgcgccgac
    12961 ccgaaagtcg tccgcgaggc ggtgctgatg ctgcgggcgc gcaagggcat ggtgctcgac
    13021 gcggtcgacc acgacacctg gagcgtgggt tcgttcttca ccaacccggt ggtcccgccg
    13081 gaggtctacc agcggctggc cggaaattcc tcaaatgtgc acgggccggt tccgcactat
    13141 ccggcgcccg acggcgtcaa gctggccgcc ggctggttgg tggagcaggc gggcttcggc
    13201 aaagggtttc cggatccgga tccgggcggc cccgaagcgc cgtgccggct gtccaccaag
    13261 cacgcgctgg ctctgaccaa ccgcggcggc gccacctccg aggacgtgat cgcgctggcg
    13321 cggacggttc gcgacggagt ccgtgatgtg tttggcgtca cattgagacc cgagcctgtg
    13381 ctgcttgggt gcaggctata gctgccaatt tcatgtggaa gcatcccgcc cgcgagttgt
    13441 cgtggcgggg tacgggtctt ttctcacggt atccttattt gtcgtgagca cttccaatcc
    13501 cccgccgatc aaccggcgct tggccttggc ggccttgggg cttggtgtgt tggctcccgg
    13561 cgttttggct gcctgctccg gcaccgcggc caagcaagcc gaaaagaagg aaaccccggc
    13621 gccgcagctg aagttccagc ctgccgacgc cgcgacggac gtggtgccga ccgccccgat
    13681 cagcgtccag atcgccgacg gctggttgca gaaagtcgcg ttgacgaact cctccggcaa
    13741 ggtcgttgcc ggggcgttca accaagaccg cacggtttac acgaccaccg agccgctcgg
    13801 cttcgacgcg acctacagct ggaccggctc ggccgtcggc catgacggca aggcggttcc
    13861 ggtggcgggc aagttcacca cggtgtcgcc gtccaagaag atcggcgggg gctttcagct
    13921 cgccgacggc cagaccgtcg gggtcgccgc gccgatcatc atccagttcg acgccccaat
    13981 cagcgacaag gccgccgtcg agaaggtgct gaccgtcacc acctccccgc ccgtcgaggg
    14041 cagctgggcg tggttgcccg acgaggcgca gggcgcccgg gtccactacc gcacccgtga
    14101 gtactacccg gcgggcgcca cggttaacgt cgacgccaag ctatacgggg tggcgttcgg
    14161 cgaaggcgcc tacggtgcgc aggacatttc gttgcacttt caggtgggcc ggcgacaggt
    14221 ggtgaaggcc gaagtctcct cgcaccgcat ccaggtgatg accgacgcgg gcgtcatcat
    14281 ggacttccca tgcagctacg gcgaggccga caaagcgcgc aacgtgaccc gcaacggggt
    14341 tcacgtggtc accgagaagt actccgactt ctacatgtcc aacccggccg cgggctacac
    14401 caatgctcac gaacgctggg cggtgcggat ctccaacaat ggcgaattca tccacgccaa
    14461 cccgtcgagc gcgggcgcgc aaggcaacag caacgtcacc aacggctgca tcaatctgtc
    14521 gacatccgac gccgaggagt attaccacac cgcgatgtac ggtgacccgg ttgaggtgac
    14581 cggcagctcg atccagctgt cctactccga cggcgacatc tgggactggg ccgtggactg
    14641 ggacacctgg gtggcgatgt ctgcgttacc gcccgcgtcg gcgcatccgc cggccaccca
    14701 gatcccggtc accgcgccgg tcaccccgtc gaccgccccg actttgtcgg gcacgccgac
    14761 caccacaacg acaacgacga cctcatctag ccccggcggt taaaccgtgt ggcgctggcc
    14821 tggtcgcggg ggcgcatgac gaccaggtcc aggttgacgt gcgacgggcg cgacgcgacg
    14881 aacgcgatca cctcggcgac atcggccgcg accaacggtg tgatgccggt gtagacggca
    14941 cccgcgcgct gttggtcgcc gtcgaaacgg accagtgaga attcggtttc gacggcaccc
    15001 ggggcaatct cggtgagccg caccggcttt cccagtagct caccgcgcag cgtccggtgc
    15061 aacgccccct gggcgtgctt ggccgcggtg tagccggcgc cgccgtcgta cacctcgagc
    15121 gccgcgatcg aggtgacggt gacgatcagg ccgtcgccgg actcgatcag cttgggcaac
    15181 agcgcccggg tgacccgcag cgtgcccagc acgttggtct cccacatcca ccgccagtgc
    15241 tccaagttgg cgtcggcgac caactccagt ccccgcgcgc caccggcgtt gttgacaagc
    15301 acatcgaccc ggcccacttc ggccgccagc gcgtccaccg caccgtcgtc tgtgacatcg
    15361 gccacaattg cggttccgcc gatgtcgtcg gccagtgcat tgatgcggtc cgcccggcgc
    15421 gccacagcaa ccacgtgaaa tccttgtgcc gcaagctttt tcgctgccgc ctcaccgatg
    15481 ccggagctgg cgccggtgac caccgcgacc cgttggtgcc cccgagctgc attcaccgga
    15541 acaactctaa taagcgtgct aaattttcgg ggtgcaccac agcgccctgt tcaacaacac
    15601 cgccgcgtgt ctttgcgcgg catgtgcgtg ttgttgccgc gcactttgcc gctcctgatc
    15661 tgcaggtgtg gccaaggccg gccatcggaa ctcggacaaa ggacgctcgt gcgctcgact
    15721 tctctcactc atagccatcg ttcacccatc cgcaaaagcc acctgcgggt gcaccgccag
    15781 atcctgccgc cgtcgttgca catctccgac tcggcggcgg catccgtctt ccgcgccgtc
    15841 cggttgcgcg gcccggtcgg ccgtgacgtc atcgccgctg tcacctccct gagcatcgcc
    15901 acggtcaacc gccaggtcat cgccctgctc gacgccggac tcctgcgcga acgcgccgac
    15961 ctcgcggtct ccggggccat tggtcgtccg cgggtgcccg tggagatcaa ccacgaaccg
    16021 ttcgtgacgc tgggcatcca catcggcgca cgaaccacca gcatcgtggc caccgacctg
    16081 ttcggccgca cgctggacac ggtcgagacg ccgacgccgc tgtctcccgc cggccccgcg
    16141 ctggcgtcgc tggccgagag cgccagccga tacctgcggc gctggcaccg ccggcgcccg
    16201 ctgtgggtcg gggtggcgat cggtggcacc gtcgacagca acaccggcca cgtcgaccac
    16261 ccgcggctgg gctggcggca ggcgccggtc gggccggtgc tggccgacgc gctgggtctg
    16321 ccggtgtcgg tggcctccca cgtcgacgcc atggccggcg ccgagctgct gctcggcctg
    16381 cggcgattcg cgccgagttc gccgaccagc ttgtacgtct acgcccgcga gaccgtggga
    16441 tatgcgctgg tgatcggcgg gcgggtgcat tgcccggcca gcggcccggg caccatcgcg
    16501 ccgctgcccg cctactctga gctactcggc ggcagcgggc agctggaatc caccgtcagc
    16561 gacgaggcgg ttctggccgc tgcccgccgg ctcaaaatcc ttcccagcgt cccgccggct
    16621 gccccggcga ctcgcaccaa cggatccacg tcggccatga ccgacttgct gcgggtggct
    16681 cgggccggaa accagcaggc cagggatctg ctggcggaaa ggggccgcgt tctcggtgag
    16741 gcggtggcgt tgctgcgcga cctgctcaac cccgacgagc tggtggtcgg tggtcaggct
    16801 ttcaccgagt acccggaggc gatggagcag gtggaggcga cgttcgctgc gcgctcgatc
    16861 ctggcgcccc gcgacatccg cgtcaccgcg ttcggcaacc gggtgcagga ggccggggcg
    16921 gggacggtgt cgctgggcgg gctctacgcc gacccgctgg ggtcgatgcg gcgggccggg
    16981 gtgctcgact ccgggctgcg cgatgtcgcc gccgacgaat cgtccgcctg acgcgacccc
    17041 gcggccagcg cacttgggtt ggttggcggc ccgtcctgta aagatgaagg cgtgcggcac
    17101 gacgacgtct tccggctgaa ggacccgcgc cgcgtcgcgg tgttggcggt gcacacctcg
    17161 ccgctggccc agccgggaat cggcgacgcc ggcggcatga acgtctacgt actgcaaacc
    17221 gcgctgcatc tggcccggcg tggcatcgag gtggagatct ttacccgggc caccgcgtcg
    17281 gccgatccgc cggtcgcccg ggtggcgccc ggcgtgctgg tgcgcaacgt ggtggcgggg
    17341 ccgttcgagg gcctggacaa gtacgatctg ccgacccagc tgtgcgcgtt cgcggccggg
    17401 gtgctgcgcg ccgaagcgtc ccacgaaccg ggttactacg acatcgtgca ctcgcactat
    17461 tggctgtccg gacaagtcgg ctggctggcc cgcgaccgct gggcggtgcc gttggtgcac
    17521 accgcgcaca cgctcgccgc ggtgaagaac gccgccctgg ccgacggaga tgcgcccgag
    17581 ccgccgctgc gcacggtcgg cgagcagcag gtcgtcgacg aagcggaccg gctgatcgtc
    17641 aacaccgaag acgaagcgaa acaattgatt tcgattcacc acgccgatcc ggcgcgcatc
    17701 gacgtggtcc accccggtgt ggacctggac gtgttccggc cgggtgaccg ccgggcggcc
    17761 cgggccgcgc tgggactccc gctcgacgag gacgtggtgg ctttcgtcgg gcggatccag
    17821 ccgctgaagg cgcccgacat cgtgctgggc gccgccgcga aactgcccgg ggtgcgcatc
    17881 gtggtggccg gaggaccgtc gggcagcggc ctggcttccc cggacggact cctccggttg
    17941 gccgacgaac tgggcatcac cgatcgggtg acattcctgc cgccgcagtc ccgcaccgac
    18001 ctggctacgt tgttccaggc cgccgatctg gttgcggtgc cgagctattc ggagtcgttc
    18061 ggcctggtcg ccatcgaggc gcaggcgagc ggaacgccgg tggtggccgc cgcggtcggt
    18121 ggcctgccgg tagcggtgcg ggatgggatc tcgggcacct tggtgtccgg tcatgacgtc
    18181 ggtgactggg ccgctgccct ggatcggctg ctgcggctcg gcgcaccgca ggccgacggg
    18241 atgagtcgcg ccgccgtcgc ccacgcggcc aacttctcct gggagaacac caccgacgcg
    18301 ctgctggcca gctatcggcg cgcgatcggc gacttcaccg cacgccagcg ccggggtgcc
    18361 gtgaaaccgg accgggcatc gatgcgcaag ccacgccgct ggacatcgcg tcgcggggtg
    18421 gacgcatgac ttctgccgac gacgatgggg tgggtgtgcg gcacgctttc gggggagtgg
    18481 gggtactgcc cgcgagcggg ggatggcgct gatgggcgcc accgtgcagg acgtcatcga
    18541 gcacacgctg caggccagcg agctgaccta ttcgaaacac gaaggggcac acggcggacc
    18601 gccggggctg gttgtcgaac tgcccggcga gcgcaagctc aagaccaaca ccatcctgag
    18661 catcggcgag cattcggtac gtgtcgaggc gttcgtgtgc cgtaagcccg acgagaacca
    18721 cgagggtgtc taccggttcc tgctcaagcg caatcgtcgg ctgtacgggg tcgcctacac
    18781 gctggacaac gtcggtgaca tctatctggt gggccgcatg tcgctggcgt cggtcagccc
    18841 agaggagatc gaccgggtgc tcggccaggt gctcgaagcg gtggattcgg actttaatac
    18901 gttgctggag ttgggttttc gctcgtcgat ccagaaagag tgggagtggc gggtgtcccg
    18961 tggggagtcg ctgaagaacc tggaggcgtt cgctcacctg atcgacgacg aagacgacga
    19021 tgcagagcgc ggagcgcgat gaggtggggg caccacccgc ttgcggggga gagtcgagca
    19081 agtaacgacg aagacgacga tgcagagcgc ggagcgcgat gaggtgggtg gagtagttcc
    19141 gcgagcgtaa cgtggctgcg attttcgggc cagaatttcg cggtggcgtt acgctcggcg
    19201 aacctcagaa tctgcgtgag agactagcgg catgggagac actgccacgc tggtgctgct
    19261 gcgccatggc gagagcgact ggaacgccct caacctgttc accggctggg tggacgtcgg
    19321 tctgaccgaa aagggccggg ccgaggcggt tcgaagtggc gagctgttga ctcaacacga
    19381 tctgttgccc gacgtgctct acacctccct gctccggcgc gcgatcagta ccgcgcacct
    19441 ggcactggac accgccgacc ggttgtggat tccggtgcgg cgcacttggc gtctcaacga
    19501 gcgtcactac ggcgcgctgc agggcctgaa caaggccgag accaaggagc gctacggcga
    19561 ggagcaattc atggcctggc gccgcagcta tgacacgccg ccgccaccga tcgagcaagg
    19621 cagcgagttc agtcaggacg ccgatccccg ctacgccaac atcgggggcg gcccgctgac
    19681 cgaatgcctg gccgacgtgg tggtccgttt cctgccgtat ttcaccgacg tcatcgtgcc
    19741 ggatctgagg accgggaaga cggtgctgat cgtcgcgcac ggcaactcgc tgcgtgccct
    19801 ggtcaagcac ctggaccaga tgtccgacga cgacatcgtc gggttgaaca tcccgaccgg
    19861 agttccgctg cgctacgacc tggatgccga catgcgtccg ctgctgccgg gcggcaccta
    19921 tctggatccg gaggccgccg ccgccggtgc cgcggcggtt gccggccaag ggcgcggctg
    19981 agtttgcccg caggcccgaa cgtggcttta aaggcctgtt cgccaaacat cgtgtgaacg
    20041 caagcggaac acctatagcg cagaatatga cttgtctgat tttggcctcg ctccgctagg
    20101 gtggcgttca ccagatttgt aggatttcgt tgtgactatg ttctcggcgc tgttgctggc
    20161 cggggtcttg tccgtgctgg cgctggccgt aggtgttgcg gtcgggaccc ggctatcgcc
    20221 ccgcgtggtc caacggcgcc agcacgtgtc caccgagtgg acggggatca cggtcgcgca
    20281 gatgctgcag cgcatcgtcg cgctgatgcc gctgggcgct gcggtggtgg attcgcatcg
    20341 cgacgtcgtt tacctcaacg atcgggccaa ggagctgggc ctggtgcgcg atcggcagct
    20401 cgacgatcag gcgtggcagg ccgcgcagca ggcgctcaac ggcgtcgaca ccgagttcga
    20461 cctgcgaccg ggtaaacgcg cggcgggccg gtccgggcta tcggtgcacg ggcaagcgcg
    20521 actgctgagc gaggaagacc gccgcttcgc ggtggtcttc gtgcacgacc agtccgacta
    20581 cgcccgcatg gaggcgacca gacgcgattt cgtggccaac gtcagccacg agctcaagac
    20641 cccggtcggc gccatggccc tgcttgccga ggccctgctg gcctccgcgg acgaccccga
    20701 aaccgttcgc cggttcgccg agaaggtgct gatcgaagcc aaccgtctgg gggacatggt
    20761 cgccgagctg atcgagctgt cccggctgca gggtgccgaa cgcttgccca acgtcaccga
    20821 tgtcgaggtc gacatcgttg tgtccgaagc gatttcacgc cacaaggtgg cagctgacaa
    20881 cgctcacatc gaggttcgca ccgacgcgcc cagcggcctg cgggtgctgg gggacgaaac
    20941 gctgctggtg accgccctgg ccaacctggt ctccaacgcg atcgcctact cgccgccggg
    21001 ttcgccggtg tcgatcagcc gtcgccgccg gggtgacaac atcgagatcg ccgtcaccga
    21061 ccgcggcatc gggatcgccc tggaagatca ggagcgggtc ttcgagcggt tcttccgcgg
    21121 cgacaaggcg cgctcgcgcg ccaccggcgg cagcgggctg gggctggcca tcgtcaaaca
    21181 cgtcgcggcc aaccacagcg gcagcatcgg cgtgtggagc aagccgggaa cgggttcgac
    21241 gttcaccttg tcgattcccg cttaccagga cagcgacgag gcgcccgagc aaccgcgggg
    21301 tcgtgaggtg cggcccaaca ggtcacaacg agaggaagaa ctgagtcgat gacccgcgag
    21361 caggaggagt ggcgcccgtg accagtgtgt tgatcgtgga ggacgaggag tcgctggccg
    21421 atccgctggc gttcctgctg cgcaaggagg gcttcgaggc cacagtggtg accgacggcc
    21481 ccgcggcgct cgccgagttc gatcgcgccg gcgcagacat cgtgctgctc gatttgatgc
    21541 tgccgggtat gtcgggaacc gacgtgtgca agcagctgcg tgcccgttcc agcgttccgg
    21601 tgatcatggt gaccgcccgc gacagcgaga tcgacaaggt ggtcggcctg gagctgggcg
    21661 ccgatgacta tgtgaccaaa ccctattcgg cgcgtgagtt gatcgcccgg atccgggcgg
    21721 tgctgcgccg cggcggcgac gacgactccg aaatcagcga cggcgtgctg gagtcggggc
    21781 cggtccgcat ggacgtcgag cgccacgtcg tctcggtgaa cggcgacaca atcaccttgc
    21841 cgctcaagga attcgatctg ctcgaatacc tgatgcgcaa cagcggacgg gtgcttaccc
    21901 gcgggcagtt gatcgaccgg gtgtggggtg ccgattacgt cggtgacacc aagacactcg
    21961 acgtccacgt caagcggctg cggtcgaaga tcgaagccga cccggccaat ccggtacacc
    22021 tggtgacggt gcgggggctg ggctacaagc tcgaaggcta gctagcgcta gcgcgacggc
    22081 tccgccggca gagggccgtt ggtgtcgaag gcggtccggg catcgtcgct cgcgcccaac
    22141 gtaatacgcc ggcgcatgtc ctcggtcatc ggtggaagcg tgtcgaggtc gaaccactgc
    22201 gccgcggtgc tctcgtcgtc ggccgcaaac ggctccccgg acagccaccg catccggaac
    22261 acgagatcaa ggtactgggc ctggtcgccg ttggcgtgta cggtcggccg ggtcacgtgc
    22321 acccaggcga gtcgggtggc ctgcgctctg accccggttt cttcgctcac ctcgcgggcg
    22381 gcgcaatccg ccggattctc gccaggttcg acgatccccg tcaccgccgt ccaggcgccg
    22441 ttgtccgagc gcttgaccag caatactttt cggtcgtgga tggtgacagc cgtgacgccc
    22501 gccagccaca gcggtgcatc gccgatcgcg cgacgcaact caacgatgaa gtcggggacc
    22561 ggcactcacc gttcttaaca cacgggtgtc gacggacggc tcgctagcgt cccccaagac
    22621 gcacactcga ttagcttgcg ccggcggcct ggtcgactat ttcgtcggcg acgcccagcg
    22681 ccagcgccat gatggacagc tgcgggttta cctccggaca gctgggcagg atcgacgcgt
    22741 cggcgaccca cacgccttcc gcgccgcgta ggcgcccggc cgcatcgacg ggacagagct
    22801 gctcgtcggc gcccgcggcg gcggtgccgg tgggatggaa ggcggccagg
//
LOCUS       DASSGQ010000105         2669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1040379,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000105 DASSGQ010000000
VERSION     DASSGQ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040379"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VFI55_04885"
     CDS             complement(<1..449)
                     /locus_tag="VFI55_04885"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HET7073404.1"
                     /translation="MSHHFDSPTAIQDGRLNLCDVFAFPGRAASSVLILTVNPDAGRS
                     SPTTFRPDAVYEFVIAADPEDQESVCLRVRFAEPTADGNQALRVLKAAGDEGVDRGTE
                     IGRGTTDGVSRFDVGRVRGSLWAGVAADPFWADGIALAGLLGSIGEGR"
     gene            complement(578..1645)
                     /locus_tag="VFI55_04890"
     CDS             complement(578..1645)
                     /locus_tag="VFI55_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012832538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET7073405.1"
                     /translation="MSVAAALVLRDGDKSRLTALLRAPTVEAGLAARARIVLLAAEGL
                     PNAEIARQVGVSRPTVIGWRARYDSGGIAALRDLDRSGRPPVIDDVAVVVATVQGPPE
                     SLGVTHWSARLLGRHLGVSFASVARIWREWKLQPWRRETFKFSTDPELDAKVRDVVGL
                     YLNPPQNAVVVCVDEKSQVQALDRTAPILPMRPGLPEKATHDYVRHGTTSLFAALEVA
                     TGKVTDACYDRHTHVEFLDFLKLVAKAYPRVRLHVVADNYATHKHPAVNTWLAKHPRV
                     TMHFTPTSGSWLNMVEVFFGIITRQAIRRGSYNSVKDLIAAIETYIDGWNERCQPFVW
                     TKTADQILAKAQRKKTSNTRH"
     gene            1746..2649
                     /locus_tag="VFI55_04895"
                     /pseudo
     CDS             1746..2649
                     /locus_tag="VFI55_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558197.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          473 a          914 c          828 g          454 t
ORIGIN      
        1 cgaccctcac caatcgagcc gagcaagccg gccaaggcga tgccgtccgc ccagaacgga
       61 tccgcggcca caccggccca caatgaaccg cgcacccggc cgacatcgaa tcggcttacc
      121 ccgtcggtcg ttccgcggcc gatctcggtt ccacgatcga cgccctcatc gccggccgcc
      181 ttcagaaccc gcaacgcttg attcccgtca gcagtcggct cggcaaatcg cacgcgcagg
      241 cacacgctct cttggtcctc agggtccgcg gcgatgacaa actcgtaaac ggcgtcgggc
      301 cgaaaggttg tcggcgacga gcgtcctgcg tccggattca ccgtcaagat cagtaccgag
      361 cttgccgccc gtcccggaaa ggcaaagacg tcacagagat tcagtcgtcc gtcttgaatc
      421 gctgtcgggc tatcgaaatg atgcgacatg ggaggtcctt ggcgtcggca gggagtgctc
      481 atctgttcgg gccgatgcga tgacccgcat tgacctcaat cgttagtccg ctaaatggat
      541 cctgtgttac gcgatctcgt ctagccgaat tgctgagcta gtgtcgcgtg tttgaagtct
      601 tcttacgctg ggctttcgca aggatctggt cggcggtctt ggtccagaca aagggctggc
      661 agcgttcgtt ccagccgtcg atgtaggtct cgatggcggc gatgaggtct ttgaccgagt
      721 tgtagctgcc gcggcggatg gcttgccggg tgatgatccc gaagaagact tccaccatgt
      781 tcagccacga cccggaggtt ggggtgaagt gcatcgtgac ccgggggtgt ttggccagcc
      841 acgtgttcac ggccgggtgt ttgtgcgtgg cgtagttatc ggcgaccacg tgcaacctca
      901 cccgcgggta ggccttcgcg acgagtttga gaaagtcgag gaactcgacg tgggtatgcc
      961 ggtcgtagca ggcgtcggta actttcccgg tcgcgacctc cagcgccgcg aacaggctgg
     1021 tcgtcccgtg gcggacgtag tcatgggtgg ctttctccgg cagcccgggc cgcatcggca
     1081 agatcggggc ggttcggtcc agcgcctgca cttggctttt ctcgtccacg cacacgacga
     1141 cggcgttctg cggcgggttc aagtacagcc cgacaacgtc acggaccttc gcgtccaatt
     1201 ccgggtcggt ggaaaacttg aacgtctccc gccgccacgg ctgcagcttc cactcccgcc
     1261 agatccgggc caccgacgcg aacgagaccc ccagatgccg gcccaaaaga cgggccgacc
     1321 agtgcgtcac ccccagcgac tccggcgggc cctgcaccgt cgccaccacc accgcgacat
     1381 catcgatcac cggcgggcga ccggaccgat ccagatcccg cagcgccgca atcccaccgc
     1441 tgtcataccg cgcccgccat ccgatcaccg tcggacgcga caccccaacc tgacgggcaa
     1501 tctcggcgtt aggtaaaccc tcggccgcca gcagcacaat ccgcgcccga gccgccaacc
     1561 cggcctccac cgtcggcgca cgcagcagcg ccgtcaacct cgacttgtca ccatcacgca
     1621 gaaccagggc tgcagcaacc gacacgcctc attgtcccga accccacacc gtaaatccac
     1681 ttccgacacg caacactagg gcgtgtcctg tggatcttgt tggcgggttg cggtagctgt
     1741 ttggtgtgct tgatcgtcat gatctgaccg agcaggagtg gtctcgtctt cggccgttgt
     1801 tgccggatcg ctcgccgcga cgtggtggcc ggtgggccga tcatcgggcg gtgatcaacg
     1861 gcgtgttgtg gcggacccga acgggttgtc cgtggcggga tctgccgtcg tgtttcggga
     1921 attggaagac ggtctataac cggcaccgac gctggtcggc ggacgggacg tgggcgcgga
     1981 tcgtgacgga gttgcgtcgc ggctgcgacc aggccgaggg ccaggcgtgg acggtcgcgg
     2041 tggattccac ggtggtgcgg gcgcatcagc acgcggcagg cgcccgccgg gcggcaccag
     2101 ccgatatcgg gggcaacgtc gaattacaag aatcccgtcg acccgggcgg cgctgacccg
     2161 cctgagggcc cgacccggga ggcgctgggc cgttcccgcg gtgggttgag cacgaagatc
     2221 cacctcgccg ccgacacccg ctgccggccg atctcccgag cgaccaccgg cgggcaccgg
     2281 cacgactcga tcgccttcga cgcggtcatg gctggcattc gtgtgccgcg cccggccggg
     2341 cgaggcagac cacgaaccag acccgacacc gtgctcgccg acaaggccta ctccgcgggc
     2401 ccgatccgcg acccgctgcg ccgccgccgg atccaggccg tcatcccggc caaggccaac
     2461 gaacacctcg cccacgccgg ccatttcgac gccgcggcct accggcaacg caacgttgtc
     2521 gaacgatgca tcaacaaact caaactgttc cgcgccctgg ccacccgcta cgacaaacgc
     2581 aactacatct atctcggcac aatcgatgtc gccacaatca agatctggct tcgcgacccc
     2641 gttccatgat ccacgggaca ggtcctagt
//
LOCUS       DASSGQ010000106         4785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1222935,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000106 DASSGQ010000000
VERSION     DASSGQ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222935"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /locus_tag="VFI55_04900"
     CDS             complement(<1..1398)
                     /locus_tag="VFI55_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HET7073406.1"
                     /translation="MNEPPALVFEEQRFSLPQLDALVDGLTATLVKLRVGAGRRVAVM
                     ASNRPEFVAALLATWRLGATAVLISPSWKRAEVDHALALTGPSHAVGDHPVLAGLMPM
                     LHLDEPITSGTPMPCKPAPSGDALLIFSSGTTGLPKAVRHTHASVGAAVRHWRDALQL
                     TPQDRIQVATPPSHILGLLNILTALRSRAPVRLHRRFDVNQMLHHIENDRITIEMAVA
                     PIAMAIASHPLLESYDLSSLRFIVWGATPVSTGVAETVTRRTGVGWVPAYGTTELPVI
                     ACNPLTGGRLDAVGRPVPGVNLRVVSLKTGRPVRPGQVGEIQARSMSLMAGYLPAEAT
                     DEAICDGWYRTGDVGSLDADGWLRITDRRKEMIKVRGFQVAPAEIETVLHGHPAVEDC
                     AVFGIPDGVNGEAIVAAVTARTPVDAADLVASVDNRLASYKHLSRVVFVDDIPRLPSG
                     KVLRRVLKEQYGCTPD"
     gene            1533..1871
                     /locus_tag="VFI55_04905"
     CDS             1533..1871
                     /locus_tag="VFI55_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET7073407.1"
                     /translation="MTVTRIIEETPRVADPASNGAVAGTVTIHLDRKKVSVPLVPGET
                     LLESARRAGLDPPFNCEAGNCGTCMARLTEGHATMRVNDALEDDEVAEGYILTCQGVP
                     DTDSITVRYE"
     gene            1950..2270
                     /locus_tag="VFI55_04910"
     CDS             1950..2270
                     /locus_tag="VFI55_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073408.1"
                     /translation="MAKGIILVESRPSSPDRDHEYNTWYDEVHLGELVALDGFVSARR
                     LRPVDADGPYVALYEVEADDLRSVLQNMLSNAGRLHMSDALQLDPPPIMRLLEVTTEY
                     GPKS"
     gene            complement(2310..2675)
                     /locus_tag="VFI55_04915"
     CDS             complement(2310..2675)
                     /locus_tag="VFI55_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073409.1"
                     /translation="MADTVVDAARQALPPEFTAQAWQLLGPDKLLVVGLSVPPDADRD
                     ETKAAARETVSGRVDSTAVAAELRAHLTAGADHVTLLLPIGYDFASGIGQLEQLAPVL
                     VGLGDRSRASRCRHVRPPG"
     gene            2803..3123
                     /locus_tag="VFI55_04920"
     CDS             2803..3123
                     /locus_tag="VFI55_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073410.1"
                     /translation="MPRVRHSDPAWSAFWRIDPRPDGRRAPDGPFIEAIEDKFEFIAL
                     GQAVAITAGFHGNSLRPDITTVPLEGVEASHVVLASRAGDRSRLVAAFRKLAQAHLTR
                     PASA"
     gene            complement(3137..4177)
                     /locus_tag="VFI55_04925"
     CDS             complement(3137..4177)
                     /locus_tag="VFI55_04925"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET7073411.1"
                     /translation="MTPGDTGPLAGIRILEVGTMLAGPYATMLLADLGAEVTKIEPRG
                     GEISRRVGSSYFASLNRNKSSVSLDLNSQSGQQHLGELVATSHALLVNLKPSAIRRLG
                     LTYDALRGWNERIVCVSITGFGLDGGDDPAFDYVVQAGVGIAALTGDPDGPPTLPGYS
                     SADNSTGMCAALGLLAKIISGTGGQVDVSLRDVMLSQLNYHASAYLNNGVEPQRHPNG
                     AHSYYVPAQLFSTANGYLALFITHDGFWKSFAAEANIDGFEGMAERVERRDEVLGLVA
                     SALATDTAAGWERRLRPLGVPAAAVRTLPEALNATPEVVVTAGDFRLVGNPIRVSGYR
                     PDYRPPPELGDG"
     gene            complement(4174..>4785)
                     /locus_tag="VFI55_04930"
     CDS             complement(4174..>4785)
                     /locus_tag="VFI55_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7073412.1"
                     /translation="PAAYTRDYLDVLDAAFTSLGPVDVENDTFRVHNPLDLAPVAPLP
                     VLVAALGPVMLSLAGERADGTILWMADERAVAEHVVPRITKAADNAGRPAPRIVAGVP
                     VCLCATGEVDSARERANRILGEAEVSPNYQRLLAYGDAKDVGDICAAGDEGAILARLR
                     RFADAGVTDLSVRLLPIGDNRDELVASKRRTREMIAALATELR"
BASE COUNT          826 a         1572 c         1606 g          781 t
ORIGIN      
        1 gtcaggcgta catccatatt gctccttgag gactcggcgc agcaccttgc ccgacggcag
       61 ccgcggaata tcgtccacga acacgacacg gctcaggtgt ttgtaggagg caagcctgtt
      121 gtccacgctg gcgacgaggt cagcggcgtc aaccggggtg cgcgccgtga cggcggccac
      181 gatcgcttcg ccgttgacac cgtcgggaat cccgaacacc gcacagtctt cgacggccgg
      241 atggccgtgc agcaccgtct cgatttcggc gggcgcgacc tggaaaccgc gcaccttgat
      301 catctctttg cggcggtcgg tgatccgcag ccaaccgtcg gcgtccaacg agccgacatc
      361 cccggtccga taccaaccgt cacagatcgc ctcgtccgtc gcctcggccg ggaggtagcc
      421 ggccatgagc gacatcgacc gggcctggat ctcaccgacc tgcccggggc ggaccggccg
      481 gccggttttc aaggagacca cgcgcaggtt cacgccgggc accgggcgcc cgacggcatc
      541 cagccggccc cccgtcagcg gattgcacgc gataacgggt agttccgtgg tgccgtaggc
      601 gggcacccaa cccacgccgg tgcgccgggt tacggtctca gcgacgccgg tgctgaccgg
      661 cgtcgcgccc cacacgatga aacgcaggga agacagatcg taggactcga gcagggggtg
      721 cgacgcgatg gccatcgcga taggtgctac cgccatctcg attgtgatgc ggtcgttttc
      781 gatgtgatgc agcatctggt tgacgtcgaa gcggcgatgc agccggacgg gcgcgcgact
      841 ccgcagcgcg gtgaggatgt tgagcaggcc gaggatgtgc gacggcggcg tggcgacctg
      901 aattcggtcc tgcggtgtca gctgcagcgc atcgcgccag tgtcggaccg ccgcgccgac
      961 cgacgcatgc gtgtgccgca ccgctttcgg cagaccggtg gtgcccgagc tgaatatcag
     1021 cagcgcatcg cccgagggcg ccggcttaca gggcatcggc gttcctgaag tgatcggttc
     1081 gtccaggtgc agcatcggca tgaggccggc cagtaccgga tggtctccga cagcgtgcga
     1141 cgggccggtc agcgcaagcg cgtggtcgac ttcggcgcgc ttccaggacg ggctgatcag
     1201 taccgccgtg gcacccaggc gccaggtcgc cagcaaagcg gcgacgaatt ccggacggtt
     1261 ggaggccatc accgcgacgc gccgacccgc gccgactcgg agtttgacaa gcgtcgcggt
     1321 cagtccgtcg accagcgcgt cgagctgagg caggctgaat cgctgttcct cgaacacgag
     1381 cgcgggcggc tcgttcacgt cccgtccttc cctggcaggc gcggccgacc cgacctccgc
     1441 gcagtctcga gaagttccta tcgcctagcg agaatagtat tctctatact gaagaacgca
     1501 agtactggcc tggttcgaag ggggcggtca tggtgacggt cacccggatt atcgaggaga
     1561 ctccacgggt ggcagacccc gcgtcaaacg gtgccgtggc aggcaccgtg acgattcacc
     1621 tcgaccgcaa gaaggtgtcg gtccccctgg tgcccggcga gaccctcctg gaaagcgcgc
     1681 gacgggcggg gctggatcca ccgttcaatt gcgaggccgg caattgcggc acatgcatgg
     1741 ccaggctgac agagggccac gcgaccatgc gagtcaatga cgctctcgaa gacgacgagg
     1801 tggccgaggg ctacattctg acgtgccagg gcgtacccga tacggattcg atcacggtgc
     1861 gctacgagta gtccgcccgg cgacgatgcg gcccgcgaag cggggcgaga aggagccggg
     1921 caattcggcc aaaggagagg cggcccaaga tggccaaggg catcatcctg gtggagagtc
     1981 gacccagttc gcccgatcgc gaccacgagt acaacacctg gtacgacgaa gtccatctgg
     2041 gcgaactcgt ggctctcgac gggttcgtct cagcgcgtcg gctgcgcccg gtcgatgccg
     2101 acggcccata tgtcgctcta tatgaggtcg aggccgacga cctgcggtcc gtcctgcaga
     2161 acatgctctc caacgccggc cggctgcata tgtctgacgc cctccagttg gatccaccgc
     2221 cgatcatgcg actgctcgag gtgacaaccg aatacggccc aaaaagctag gcggagtggg
     2281 ctgcccggac ctcgaatgtg gcgctgcagt caccctggag gccgaacgtg acggcagcgt
     2341 gacgctcgag acctgtcccc gagtccgacc agcactggag ccaattgttc gagttgcccg
     2401 atgccagagg caaagtcgta gccgatcggc agcagcagcg tcacgtgatc ggcgccggcg
     2461 gtcagatgcg cgcgaagttc tgcggcgaca gccgtcgagt cgactcgacc cgaaacagtc
     2521 tcccgtgcag cagctttcgt ctcatcgcgg tcagcgtccg gaggcacaga tagcccgacg
     2581 actaggagct tgtccggccc gagcagctgc caagcctgcg cggtgaactc cgggggcaac
     2641 gcctgccggg ccgcgtcgac cacggtgtca gccacgactt ttctacgtca ctcgccgaag
     2701 tagtgacctt cgtcgaggtc ttcgatcagc ccgggccgtt cggggtgcca gtcaagcact
     2761 ttctgcgtca gggcacttga cgtcggattg tccgacgagc ccatgccgcg ggtgcggcac
     2821 tccgatccgg cctggagtgc cttctggcgc atcgatcccc ggcccgacgg gcgtcgcgcc
     2881 cctgatggtc cgttcatcga agcgatcgag gacaagttcg aattcatcgc cctcgggcag
     2941 gcggtggcca tcacggcggg ctttcacggc aacagcctgc gccccgacat caccacggtc
     3001 cccttggaag gtgtcgaagc cagccatgtc gtattggcaa gccgtgccgg ggaccgtagc
     3061 cgtctggtgg cggcctttcg caaactcgcc caagcccacc tcactcgtcc cgcctcggcg
     3121 taagtccccg cggtgtctag ccgtcgccca gctcgggtgg cggtcgatag tccggtcgat
     3181 aaccggaaac gcggatcgga ttcccgacga ggcggaaatc acctgccgtc acaaccactt
     3241 cgggcgtggc gttgagcgct tccggcagcg tcctgacggc cgccgccgga actccgagcg
     3301 gacgcagtcg ccgttcccag ccggctgcgg tgtcggtggc cagcgctgag gcgacgaggc
     3361 caagtacctc gtcgcgccgc tccacccgtt cggccatccc ttcgaaaccg tcgatattcg
     3421 cctcggcggc aaaggatttc cagaacccgt catgggtgat gaatagcgcc agataaccgt
     3481 tggcggtcga gaagagctgg gccggaacgt aatacgagtg cgctccgttc gggtgtcgct
     3541 gcggctcgac gccgttgttg aggtaggcgg aggcgtggta gttgagctgc gacagcatga
     3601 cgtcgcgcag cgacacatcc acttgcccac cggtgccgga gatgatcttg gcaagtaggc
     3661 ccagcgccgc gcacattccg gtggagttgt cggccgacga atagccgggc agcgtcggcg
     3721 ggccgtcggg atcgccggtc agtgcggcga tgccgacacc cgcctgcacc acgtagtcga
     3781 aagccggatc gtcgccgccg tccagcccga aacccgtgat cgacacgcag acaattcgct
     3841 cgttccagcc gcgtaacgcg tcgtaggtca gtcccagccg gcggatggcc gacggtttca
     3901 ggttgaccag cagcgcgtgg gacgtcgcta ccaattcgcc taaatgttgc tgtccgcttt
     3961 gcgaattcag gtccaggctg acgctggatt tgttccggtt gaggctggcg aagtagctgg
     4021 acccgacccg gcgcgagatt tcaccgccac ggggttcgat cttggtgacc tcggcaccga
     4081 gatcggccag cagcatggtg gcgtacggac cggccagcat ggtgccgacc tcgagaatgc
     4141 gtattcccgc cagcggcccg gtgtctccgg gtgtcatcgc aactctgttg cgagcgcggc
     4201 gatcatctcg cgggtgcggc gtttggaggc aacgagctcg tcgcggttgt ctccgatcgg
     4261 cagcagtcgc accgacagat cggtcacccc cgcgtcggcg aatcgacgca accgggccag
     4321 gatcgccccc tcgtcgccgg ccgcgcagat gtcgcccacg tccttggcgt caccataggc
     4381 gagcaggcgc tgatagttcg gcgagacctc ggcctccccc agaatccggt tggcgcgctc
     4441 gcgcgcggag tcgacttcac ctgtcgcgca aaggcagaca gggacaccgg cgacgatgcg
     4501 cggggccgga cgcccggcgt tgtcggcggc cttggtgatc cgcggcacca cgtgctcggc
     4561 caccgcgcgt tcgtcggcca tccacaggat ggtgccgtcg gcccgctcgc cggccaggct
     4621 cagcatgacc ggcccgagcg ccgcgaccaa caccggcaaa ggcgccaccg gagccaggtc
     4681 gagcgggttg tgcactcgga aggtgtcgtt ctccacgtcg acgggaccaa ggctggtgaa
     4741 ggcggcgtct aggacgtcga ggtagtcacg ggtgtaggcg gccgg
//
LOCUS       DASSGQ010000107        15728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_493913,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000107 DASSGQ010000000
VERSION     DASSGQ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15728
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493913"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..1923
                     /locus_tag="VFI55_04935"
     CDS             337..1923
                     /locus_tag="VFI55_04935"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006895733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HET7073413.1"
                     /translation="MNKPATKTPTTTSEPRPATLSDDVVRRLLTGVRATGGMVDTIAP
                     GTGKRLFSLPQSNTADLLESFQAAREAQRAWAATPPAKRVKPFFRLADLLLSRQDEGL
                     DVIQLETGKARLHAFEEILDAVTSTLYYAREAPDLLKPARRAGSLPGFTRTYEYAHPK
                     GVVATITPWNYPMTLGMDVVPALLAGNAIVHKPDNQTALSTLWPRSLAIEAGLPPELW
                     QVALGDPALIGNDLIDNADYVGFTGSTNAGRAIGLRAASRLIGCSLELGGKNPMVVLA
                     DADLDKTANVAVRGCFANAGQLCVSIERIYADASIFDEFVKRFADTVKGLRLGFALDY
                     STDMGSLTSERQLQRVSEHVQSAVAAGAKVVAGGRARPDLGPYFYEPTILVDVPPTAP
                     LYRDETFGPVVSVYPFKSEAEAITQANDTEYGLNAAVFSRNTRHAREVASEIKAGTVN
                     INEGYAAAYTSQGAPMGGMKASGVGRRHGSGGLLKYTEAQTIASQHLIGFDPLMGMDS
                     QQHARLLTRLLQSMKALRIR"
     gene            2081..2950
                     /locus_tag="VFI55_04940"
     CDS             2081..2950
                     /locus_tag="VFI55_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HET7073414.1"
                     /translation="MSYVIAAPGMLAGTAADVAGIGSSLSEANGAAAPATTTVIAAGS
                     DEVSAAIASLFSSHGKAFQALSARAAAFHSRFVQGLIAGAGAYAGAEAANAAPFQTVT
                     LTLTLKVPVPNAAVSVGGFTLLQAGSANASSNFGGLAVAFGANSDATVTGGVFNTALA
                     IGNTSLAEAGTGNLNLATAFGTNSAAYAGLAGNLDTAVALGKNNIASAASGNFNFASA
                     LGTGSDAFAGGIRFVPGSGNVALVVGTDSTAFAGASPTTSGGNGDLAVAVGNMLDAAA
                     TGTFQWVYSAFQP"
     gene            3211..3597
                     /locus_tag="VFI55_04945"
     CDS             3211..3597
                     /locus_tag="VFI55_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073415.1"
                     /translation="MPFASGAMPAGQTIVRLPGYGGMGDVYFAQHPRSLRSQALKILR
                     TDISAHDAYRRRLVREADVPGSLGTDAATMQRDRYPVGASANQVAAVVAAIPSAVDYA
                     HRRRDKSPTRASVQAALAPQATTREA"
     gene            3675..4634
                     /locus_tag="VFI55_04950"
     CDS             3675..4634
                     /locus_tag="VFI55_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HET7073416.1"
                     /translation="MSTGITRFTGITSHAGALATAIVVLTAAAILRNSAAVADSNQDE
                     QFLALLDKEGIPALRGVPSLIYTAHNVCRELDAGMPAGGLVDAMVNDAYGIDPPERLY
                     APGRLARTEARFITAAVEAYCPYDQGKIASIVDNSGAGWNESAQRVAPYTHNALNSRG
                     DRREPPAWQEPTGTGAVRRPHLMDGGALVAGRSGGDRSDFDVHGTAFASLIGAAPSGE
                     ITDPNPPPLPAPPPSAAHIRTAPHPIAAPPPPKHLPPPPQQPPPPAAPQPGGAAGSGG
                     NGSGGSGGGDGNGGGGTGGSGGRHPAEPSPEPPMPPGFVTLAP"
     gene            complement(4924..5382)
                     /locus_tag="VFI55_04955"
     CDS             complement(4924..5382)
                     /locus_tag="VFI55_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016884659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET7073417.1"
                     /translation="MDISELASLPERVGKRLGTSRSELITQQDVQWFADATRAHEWIH
                     VDPQRAARESPFGTTVAHGYLTLSLATPFVTELLGVGDGVVGINYGLNRVRFPAPVPV
                     GSRVRAHGTLRSATPDGPGMRTVIELVYEVEGGDRPPCVAEVVSVLLPAR"
     gene            5409..5855
                     /locus_tag="VFI55_04960"
     CDS             5409..5855
                     /locus_tag="VFI55_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7073418.1"
                     /translation="MSDQDEIATLEEALVKFFAAYRRSRSRLQRDPGLVGLTNAQFAV
                     LEAVTTHGNKGVGAVAAACGMTQPPATRAIARLAAKELIERRGSAQDGRVSQLAVTPA
                     GRRLLAHHRQRLHRAASTLLDKLPPASYADAAGLLDLLGGALDELP"
     gene            5911..6605
                     /locus_tag="VFI55_04965"
                     /pseudo
     CDS             5911..6605
                     /locus_tag="VFI55_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055628.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase"
     gene            7002..7301
                     /locus_tag="VFI55_04970"
     CDS             7002..7301
                     /locus_tag="VFI55_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073419.1"
                     /translation="MSNHHVNLTQQESSLIGESHPDVLERMDDKQLKDLQSRLRNARE
                     KNFSLLRRQGAARVEAEGSRGAAQPANERRGEKVEVFDEALARVNQRLESIRVTE"
     gene            complement(7733..7815)
                     /locus_tag="VFI55_04975"
     tRNA            complement(7733..7815)
                     /locus_tag="VFI55_04975"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7780..7782),aa:Leu,seq:cag)
     gene            8067..9734
                     /locus_tag="VFI55_04980"
     CDS             8067..9734
                     /locus_tag="VFI55_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HET7073420.1"
                     /translation="MSSQKGLVQRIERKLEATLDNAFARVFGGSIVPQEVEALLRREA
                     EDGVQALHGNRLLAPNEYIITLGMHDFEKVAAESDLKSRGFATYLADYIHEKGWQTYG
                     DVVVRFEQSSSLHTGQYRARGAVNPDVEPRPTVNHHLRPQSDHAFGAERGVAPMTDNS
                     SYRGGQGQGRPGDEYYDDRYGRPQEEPRSQDPQGAPDPRAGYPPEQGGYPPQGGYAPP
                     RHPDQGGYPEQGGYPEQGGYPGQGGYPDQGGYQGQRGGYPDQGGYDQRGYQDQRGYQD
                     PGRGGYPDQGQGGYPPPYEQRPPAPGGYGGQGYDQGYRQGGYAPPAAPGGGQPGAGQQ
                     GYGGYGDYGRGPARPEEGGYAPAAPPAPEQQRPPYPDQGGGYDQGYQQGGGYGQQDYS
                     QGDYTQYAENAPGGGYAAPQGGYAETGHRDYGQPADYGQQADYGQPADYGQQAGGGYG
                     GYGQGGYGSAGTSVTLQLDDGSGRTYQLREGSNIVGRGQDAQFRLPDTGVSRRHLEIR
                     WDGQVALLSDLNSTNGTTVNNAPVQEWQLADGDVIRLGHSEIIVRIH"
     gene            9859..10326
                     /locus_tag="VFI55_04985"
     CDS             9859..10326
                     /locus_tag="VFI55_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET7073421.1"
                     /translation="MQGLVLQLTRAGFLMLLWVFIWSVLRILRTDIYAPTGAVMVRRG
                     LALRGTLLPSRQRRHAARYLVVTEGALTGGRITLSGQPVLIGRADDSTLVLTDDYAST
                     RHARLSQRGSEWYVEDLGSTNGTYLDRAKVTTAVRVPLGTPIRIGKTAIELRP"
     gene            10415..11914
                     /locus_tag="VFI55_04990"
     CDS             10415..11914
                     /locus_tag="VFI55_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET7073422.1"
                     /translation="MTLVLRYAARSDRGLVRTNNEDSVYAGARLLALADGMGGHAAGE
                     VASQLVIAALAHLDDDEPGGDLLAKLDSAVRAGNSAIAAQVEMEPDLEGMGTTLTAIL
                     FDGNRIGLVHIGDSRGYLLRDGELAQITKDDTFVQTLVDEGRITREEAHSHPQRSLIM
                     RALTGHEVEPTLTMREARAGDRYLLCSDGLSDPVSDETILEALQIPDVAESAYRLIEL
                     ALRGGGPDNVTVVVADVVDYDYGQTQPILAGAVSGEEDQMTLPNTSAGRASAIRPRNE
                     VAKRVPPQAETPKRPRWSRRRMAIVVTLVVLLVLAGVAIGRAIIRSNYYVAEYNGMVA
                     ILRGIQGSLLGMSLHEPYLIGCLNARNELSLVGSGQTGGHLDCRLMQLKDLRAPERAQ
                     VQAGLPAGTLDNAIGQLRQLSTNSLLPPCPPPRATSPPGSPVPAATSSPSGTLPNSTS
                     TGSPATTAPAAPATTAPAAPATTSPTNSQTVTALPPPSLQPGIDCRALT"
     gene            11922..13331
                     /locus_tag="VFI55_04995"
     CDS             11922..13331
                     /locus_tag="VFI55_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET7073423.1"
                     /translation="MTTQVQPPVSVTPPLPTRRNAELLLLCFAALITVAALLIVEGNQ
                     ERSLRWDLTSYGLIFLVIFGSAHLAIRRFAPYTDPLLLPIVALLNGLGLVMIHRLDLV
                     NNELSGRRHPSATQQMLWTVVGVAAFALVVTFLKDHRQLARYGYICGVAGLIFLVIPA
                     LLPASLSEQNGAKIWIRFPGFSIQPAEFSKILLLIFFSAVLIAKRRLFTSVGKHFMGM
                     TLPRPRDLAPLLAAWVISVGVMTFEKDLGTSLLLYASFLVVVYLATQRFSWVVIGLLL
                     FAVGSVVAYFIFPHVRVRVQMWWDPFSDPDGAGYQIVQSLFSFATGGIFGTGLGNGQP
                     DTVPAASTDFIIAAFGEELGLVGLASILMLYTIVIVRGLRTAIATRDSFGKLLAAGLA
                     STLAIQLFIVVGGVTKLIPLTGLTTPWMSYGGSSLLSNYVLLAILARISHSARRPLRT
                     RARNKSPIAAAKTEVIERV"
     gene            13328..14806
                     /gene="pbpA"
                     /locus_tag="VFI55_05000"
     CDS             13328..14806
                     /gene="pbpA"
                     /locus_tag="VFI55_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-transpeptidase PbpA"
                     /protein_id="HET7073424.1"
                     /translation="MNASLRRISVTVMALIVLLLLNATMTQVFAADSLRADPRNQRVL
                     LDEYSRQRGQIVARDQLLAYSVATDSRFRFLRVYPNPAVYAPITGFYSLRYSSSGLER
                     AEDPLLNGSDERLFGRRLADFFTGRDPRGGNVDTTIVPRIQQAGWDAMQQGCGGPPCK
                     GAVVALEPSTGKILAMVSSPSYDPNLLASHDPEVQALAWQRLRDDPDNPLTNRAISET
                     YPPGSTFKVITTAAALQAGATDTEQLTAAASIPLPGSTATLENYGDAPCGSAPTVSLS
                     QAFALSCNTAFVQLGILTGADALRNTAHSFGLDTTPSVIPLQVAESTVGIIPDAAALG
                     MSSIGQKDVALTPLQNAEIAATIANGGVTMQPYLIDSLKGPDLSNISTTAPYQQRRAV
                     SPQVAAKLTELMVGAEKVAQQKGAIPGVQIASKTGTAEHGTDPRHTPPHAWYIAFAPA
                     QTPKVAVAVLVENGADRLSATGGALAAPIGRAVIEAALQGGP"
     gene            14803..>15728
                     /locus_tag="VFI55_05005"
     CDS             14803..>15728
                     /locus_tag="VFI55_05005"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET7073425.1"
                     /translation="MSPRVGVTLSGRYRLQRLIATGGMGQVWEAVDSRLGRRVAVKVL
                     KQEFSQDPEFIERFRAEARTTAMLNHPGIAAVHDYGESQLDGEGRTAYLVMELVNGEP
                     LNSVIKRTGRLSLRHALDMLEQTGRALQVAHAAGLVHRDVKPGNILITPTGQVKITDF
                     GIAKAVDAAPVTQTGMVMGTAQYIAPEQALGHDATPASDVYSLGVVGYEVVSGKRPFT
                     GDGALTVAMKHIKEPPPPLPPELPPNVRELIEITLVKNPTMRYRSGGPFADAVAAVRS
                     GRRPPRPNQAPSIGRAAPAAVPSAAQARAAADL"
BASE COUNT         2745 a         5332 c         5049 g         2602 t
ORIGIN      
        1 cccatcgcgt cgaggtggtc ggtcgcggcg gttgatcagc ggccattttg caaggacccg
       61 gccgcgcctg ttcccgtctg catatcggct ggctgaggtc gtggaatgtc gatctgcgcg
      121 ggtccccgac ggcgccatca gcggcaaagg cgtttgtgtg ggaacacaag aatcagcggt
      181 aatgccatgt agctgaacat tgtgcgtcag cagcgcaaac ccgagaatta gagggtccat
      241 gtccaactct tcggacgcgt gccgtgcggt tggtggcacc ggcgattcca ccagggccat
      301 ccggcaccag cacgcctgaa cgcttgggag ccaccgatga acaagccagc gacgaagacc
      361 ccgaccacca catcggagcc gaggcccgcc acgctgtccg acgacgtagt gcgacgactg
      421 ctcaccgggg tacgcgccac cggcgggatg gtcgacacca tcgcccccgg caccggcaaa
      481 cgcctcttct cgcttcccca gtccaacaca gccgacctgc tggagtcgtt ccaggccgcc
      541 cgcgaggccc agcgcgcctg ggcggcgacc ccgcccgcca aacgggtcaa gccttttttc
      601 cggctcgccg acctgttgct gtcccgccag gacgagggcc tggacgtaat ccaactggaa
      661 acaggcaaag cgcggctgca cgcgttcgag gagatcctcg acgcggtgac ctcgacgctg
      721 tactacgcgc gcgaagcgcc cgacctgctc aaacccgccc gccgcgccgg ctcgttgccc
      781 ggattcaccc gaacatacga atacgcgcac cccaaaggcg tggtggccac catcaccccg
      841 tggaactacc ccatgacgct ggggatggac gtcgtcccgg ccctcctcgc gggcaacgcg
      901 atcgtccaca aaccggataa ccagaccgcc ctgtcgaccc tatggccacg cagcctggcc
      961 atcgaggccg gactgccacc ggaattgtgg caagtcgctc tcggtgatcc agccctgatc
     1021 ggcaatgatc tcatcgacaa cgccgactac gtcggattca ccggatccac caatgcgggc
     1081 cgcgcaatcg ggctacgcgc cgcttcgcgg ctcatcggct gctcattgga gctgggcgga
     1141 aaaaacccga tggttgtgct cgccgacgcc gacctggaca agaccgcgaa cgtcgcggtc
     1201 cggggatgct tcgccaacgc cggccagctg tgcgtgtcga tcgaacgaat ctacgccgat
     1261 gccagcatct ttgacgagtt cgtaaagcgg ttcgccgaca ccgtcaaggg gctgcggctg
     1321 gggttcgcgc tggattacag caccgacatg ggatcactga cctccgaacg tcaactgcaa
     1381 cgcgtcagcg aacacgtcca aagcgccgtc gccgccggcg cgaaagtcgt cgcgggcggc
     1441 cgcgctcgcc cggacctggg cccctacttc tacgagccca cgatattggt tgacgttccc
     1501 ccgacggccc cgttgtaccg ggacgagacc ttcgggccgg tggtctcggt gtacccattc
     1561 aagtccgaag ccgaggcgat cactcaggcc aacgacaccg aatacggttt gaacgcagcg
     1621 gttttcagcc gaaacacccg tcacgcccgt gaggtggcca gcgagatcaa agccggcacc
     1681 gtcaacatca acgaggggta cgccgcggcg tacacctccc agggcgcccc gatgggcggc
     1741 atgaaagcct cgggggtcgg acgccgacac ggttccggtg gtctcctgaa gtacaccgaa
     1801 gctcaaacga ttgcgagcca acatctcatc ggtttcgacc ccctcatggg catggactcc
     1861 caacagcacg cccggctact cacccgactt ctgcaatcga tgaaggctct gcgcatccga
     1921 taaccgcacg gctgccgaca acggccgggc aactaccgcg tacccaccaa cacaacggcg
     1981 ggtacacgtt tccgtttagc gccccaaccg atctgcagac ctaacgcgat acgctctggt
     2041 atttcgttga gaaatcgatg actcgggtgg caggtcgacg atgtcgtatg tgatcgcagc
     2101 accgggcatg ttggcgggga cggcggcaga tgtggcgggc atcggctcgt cgctcagcga
     2161 ggccaacggg gcggccgcgc ctgcgaccac caccgtgatc gccgccggta gcgatgaggt
     2221 gtcggcggcc atcgcgtcgc tcttttccag ccacggcaag gcgtttcagg cgctgagtgc
     2281 ccgggcggcg gcgtttcatt cccggtttgt ccagggcctc atcgcgggtg cgggagccta
     2341 tgcgggtgcc gaggccgcca atgcggcacc tttccagacc gtgacgctga cgctgacgct
     2401 gaaagtgccc gttccgaatg cggccgtctc ggtcggcggg ttcacattgc tccaggcggg
     2461 cagcgcgaac gccagctcga acttcggcgg cctcgcggtc gccttcggcg ccaacagcga
     2521 cgcgaccgtc accggaggcg tcttcaacac cgcactcgcc atcggcaaca ccagcctagc
     2581 cgaggccggc acgggcaacc tgaacctcgc caccgccttc ggcaccaaca gtgccgcgta
     2641 tgccggcctc gccggcaacc ttgataccgc cgtcgccctc ggcaagaaca acatcgccag
     2701 cgctgccagc ggcaacttta acttcgcctc cgccttgggc actggcagcg acgcctttgc
     2761 cggcggcatc aggtttgtcc ccggcagcgg caacgtcgcc ctcgtcgtgg gcactgacag
     2821 caccgccttt gccggcgcca gccccactac ttccggcggc aatggcgacc tcgccgtcgc
     2881 cgtcggcaac atgctcgatg cggcggctac cggcaccttc caatgggtct actccgcttt
     2941 ccaaccatga ccggctggcg ccgcggggct tgacagcggc ggcttgcgac gggactcgtt
     3001 cgtgcttcgc gggcctgctc gccgacggtg gctcacacgc cgtggtgtgc gccagcgcgg
     3061 caacccggcc aaggcctcgc ctcgtccggt cgcgagcgcc gcatccattt ttcgcatatc
     3121 ggatgatgca ttcgcttgtg gggaatcctg cagcatagcg tcaggatttg cccataccgc
     3181 ttgtcgaagc ggaggcagta gactttttgc atgccgtttg ccagcggcgc aatgcctgct
     3241 ggacagacca ttgtgcggct gcccggatat ggaggaatgg gcgacgtcta cttcgcccag
     3301 catccccggt cgctccgctc ccaagcgctc aagatattgc gcactgacat ctccgcgcac
     3361 gacgcctacc gccggcgttt ggttagagag gcggacgtgc cgggctcgct cggaaccgac
     3421 gcggcgacga tgcaaaggga ccgctatccg gtcggcgcgt cagcaaacca ggtagccgcg
     3481 gtagtcgcag caatccccag cgcagtcgac tatgcgcatc gccgccgcga taaatcccca
     3541 actcgtgcgt ccgttcaggc cgcgctggcc ccgcaggcaa ctacccgcga ggcgtagatc
     3601 aaatatccaa agctcgtcac gtgacgggcc ctagaaagtc acgtgacggg ccctagaaga
     3661 acgggaaccc tgccatgtct accggcatca ccaggttcac cggtatcacc agccacgccg
     3721 gtgccctggc taccgccatc gtggtgctca ccgctgctgc aattctgcgc aacagcgcag
     3781 cagtggccga ctcaaaccaa gacgagcagt ttctcgcgct gctcgataaa gagggcatcc
     3841 ctgccctcag gggcgtgcca agcctcatct acacagccca taacgtttgt cgcgaactcg
     3901 atgccggcat gccggcgggt ggcttggtgg acgcgatggt gaacgatgcg tatggcattg
     3961 acccgcccga gcgcctatac gcccccggcc gcctcgcgcg caccgaggcc cgattcatca
     4021 cggcggcagt ggaggcctac tgcccgtacg accaagggaa gatcgcttcc atcgtggaca
     4081 attccggggc gggatggaat gagtcggcgc aacgtgtggc gccgtacacg cataacgccc
     4141 tcaactcgcg aggcgatcgc cgggagccac cggcgtggca ggaaccgacg ggcaccggtg
     4201 cggtgcggcg gccacacttg atggacggtg gcgctttggt ggcggggcgc tcaggaggcg
     4261 accggtcgga ttttgacgtg catggcacag cgttcgcctc gctgatcgga gcggctccct
     4321 ccggcgagat caccgacccg aacccgccgc cattgccggc accaccgccg tcggcggcgc
     4381 atatccggac agcaccccac ccgatcgcgg caccgcctcc accaaaacac ctaccgcctc
     4441 caccccaaca gccgccacct cctgctgccc cgcagccggg gggcgctgcc ggcagtggcg
     4501 gcaacggcag tggcggcagt ggcggtggtg acggcaacgg cggtggtggc actggcggca
     4561 gcggcggtcg ccacccagcg gagccgtcgc cagagccacc catgccgccg ggctttgtca
     4621 cgctcgcgcc gtgactatcc tgcgggtttg ttggcgaaaa ggacggcggt catccgcatg
     4681 gccatccgaa gaaccgtggt cgagaattcc ttgaccgggc cctgacaggg gcagtcctgc
     4741 aaccccgcac acgccggttg ccggcgtgtg cgacaaggac cggttggccg gcgcggtggt
     4801 ggcctcgcct acgaggtggt cgcaggcggc ggtgtggcca acggtgacct atcgcaatcg
     4861 cgccgccgtt gacgaagaga atccgcaaga ggcctcacat agcgccccgc acctgccccc
     4921 ctgctagcgc gcgggaagca gcacgctgac cacttcggcg acgcagggtg gccggtcgcc
     4981 gccttcgact tcgtatacga gctcgatcac tgtgcgcatc cccggaccgt cgggagtcgc
     5041 gctgcgcagc gtgccgtgcg cgcgtacccg cgagccgacg gggacgggcg ctgggaagcg
     5101 cacgcggttg agcccgtagt tgattcccac cacgccgtca ccgacgccga gcagctcagt
     5161 cacgaacgga gtggccagtg acagggtcag gtatccgtgc gcgacagtgg tgccgaaagg
     5221 gctttcccgt gcggcccttt ggggatcgac atgaatccac tcgtgcgcgc gggtcgcgtc
     5281 ggcgaaccac tgcacgtctt gttgggtgat caactccgag cggctggtcc cgagccgctt
     5341 gccgacacgc tcgggcaggg aggctagctc cgatatgtcc atacgccaac catataccct
     5401 aggtatatat gtcagatcaa gacgaaatag ccactctcga agaagcgctg gtgaaattct
     5461 tcgccgccta tcggcgctct cggtctcgcc tgcaacgaga tccaggcctg gtcgggctca
     5521 ccaacgccca attcgccgtg ctggaggcgg tgaccaccca tggcaacaag ggtgtcgggg
     5581 ccgtggcggc ggcgtgcggg atgacccagc ctccagccac ccgcgccatt gctcgcctgg
     5641 cggccaagga actcatcgag cgccgcggtt cagctcagga tggtcgcgtc agccagctcg
     5701 ccgtcacacc agcgggacga cgactacttg cccaccaccg tcaacgactt caccgcgccg
     5761 cgagcaccct gcttgacaag ctgccgcccg cgagctacgc cgacgccgcc ggactgcttg
     5821 acctgctagg cggcgccctc gatgaactgc catgaacaca acgcgggttg tgaagcggcc
     5881 cgcattctgc tcccggtcta cggtcaattg atgacggcgg atgagcatgc gcctaagcag
     5941 cggtggtcta atgcggacgg gggacgttcg taccttcgaa cgctgcctgt gttgacgggc
     6001 gtgggccctg ggttcgatgt cgacacagcc cctgcagacc cggtgtcgtt gttcgtcgcg
     6061 tggtggcagg aggccgttga tgccggcgtt cccgagcctc atgcatgacg ttgtcgacgg
     6121 tcgatgccca gggattgccg cgtgctcggg tgctgattgt gaaggcggtc gacgtgcgtg
     6181 gttggcattt cgcggtcagt tcggtgagcc agaagggctg cgaccttgca gcgaatcccg
     6241 ttgcagcgtt gaccttttac tggccttcac aggttcgcca ggtaagggta ggcggtgttg
     6301 ttgtcggtga cggcgaccag gccagcgctg aagacttcct tgctcggccg ctggggtcac
     6361 gtgcgatcac gttgacgctg agacaaagtc aaccacttgc cgatgttgcg gagttggatg
     6421 cggagatcga taaggcctac cgcaagctca gcgatgatcc tggtctggtt ccggatgaat
     6481 ggatctcgta tgcgctgcgg ccagcgcatg ttgagttctg ggagggaaca cccgaccgtc
     6541 gacaccagcg actgacttat gaacgttccg gggatggctg ggaccgtacg cggttgtggc
     6601 cgtaggagtg aatccgtgtc aagggcggca ccggatgttg ttgcggccat gacgcttccg
     6661 ctgcaggtga gtgcgattcg tctttgactg cgatggattg ctgctcgaca ccgagccgtg
     6721 ctggtcacgt gcttaggcgt cgttgtacgc caattgcgaa ccctactccg ctacgctgcc
     6781 acctgcaccc acccgacgaa ccgctcgcag agcacggcag ggaggttgga gtggccacgg
     6841 attcggggac gccgcggggt agtcgagcgg aaagcgatcg gtttggcagg cgaaccccac
     6901 gccgtctggc gatgtgaact ttagatggcc ctggcgtgac cgttcggcgt gctgtccggc
     6961 gattcgccgt gccggtggcc ggcgcaggct atgaatctac ggtgagcaac caccatgtga
     7021 acctgaccca gcaggaaagc tcgttgatcg gcgagagtca tcccgacgtt ctggagcgca
     7081 tggatgacaa gcagctgaag gatctacaga gccggctcag gaacgcgcgt gagaagaact
     7141 tcagcctatt gcggcggcag ggcgcggccc gagtggaggc cgaaggttct cgcggtgccg
     7201 cccagccagc caacgagcga cgtggcgaaa aggtggaggt cttcgacgag gcgctggctc
     7261 gggtgaacca gcgcctggag tccatccgcg tcaccgagta gcggcggcgt tttacggatt
     7321 gaacgggttc acggcgaact ggcttgaggc gtcggttgtc gaagtttgcg accactcgag
     7381 caataacggc gaggacgccg ccttgtgtcg cctagtttta tgcccactcc tttcccagca
     7441 gcgattattt tcgggatggc gccgcaacgt gcgcgcgatt gcaaagcttc attggtctgg
     7501 aggcgagccg tggtgcttcc gtagacgtgc gggcggagtc catagaaggg gttagagctc
     7561 tccgcatcga cgccgagccc accaacagca aggcgtccaa ttgggaagtt gcgacgaagt
     7621 cccgcccacc gtcccttggg cggcgtaaaa acaacagccc accaaacaca aaggtcacga
     7681 cccatttctg gttcgtgacc tttgtgcctg cagttcgtag aaccgctcag ggtgggcgaa
     7741 gggggacttg aacccccacg tcccgaagga cactggcacc tgaagccagc gcgtctgcca
     7801 ttccgccact cgcccggaaa acaaccgatg gaccatacca ctgtattagc cctgcgcccc
     7861 aaccgccccg gagcgtttgg cgaccgctca cgccgggagc tttgctagac agacgtgtct
     7921 gagctgcccg gatgcaattc tcagagttcc gaaggtgccg tggcgtcgcc gtcgcccggt
     7981 aacatgctgg ggtgatacga cacgcgggcg attcatttgc ctcgcgcccg cgacacacca
     8041 cggcaactgc gggcgagcgc taaaggatga gtagccagaa ggggctcgtg cagcgcatcg
     8101 agcgcaagct tgaagcgacc ctggacaatg cgtttgcccg agtattcggg ggatcgattg
     8161 ttccgcagga ggttgaagcc ctattgcgcc gcgaagccga agacggcgtc caggcgcttc
     8221 acggaaatcg tcttttggcc cccaacgaat acatcattac cctcgggatg cacgactttg
     8281 agaaggtggc cgccgagtcg gacctcaagt cgcgcggttt cgctacatac ttggccgact
     8341 atatccatga aaaggggtgg caaacgtatg gtgatgtggt cgtccggttc gagcagtcgt
     8401 cgagcctgca taccggtcag taccgcgccc gcggtgctgt caaccctgac gtcgagcccc
     8461 gcccgacggt caaccatcac ctccggccac aatcagatca cgcgttcggc gcagaacgag
     8521 gagtagcacc aatgaccgac aattcgagct accgcggggg tcaggggcag gggcggcccg
     8581 gcgacgagta ctacgacgat cgctatgggc gtccgcaaga ggaaccccgc agccaggacc
     8641 cgcagggcgc accggacccc cgggctggct acccgcccga gcagggcggg tacccgcccc
     8701 agggcggata cgcgcctccc cgtcatcccg accagggcgg ctatccggag cagggcggct
     8761 atccggagca gggcggttac ccgggccagg gcggttaccc cgatcagggc ggctaccagg
     8821 gccagcgcgg cggttacccg gatcagggcg gctatgatca gcgcggatac caagaccagc
     8881 gcgggtacca agaccccggt cgcggcggct atcccgacca aggtcagggt ggctacccac
     8941 cgccctacga gcagcgtccc cccgcccctg gcggctacgg cgggcaaggc tacgaccagg
     9001 gctaccgcca gggcggctac gcccctcctg ctgctcctgg cggcggtcag ccgggcgctg
     9061 gacagcaggg ttacggcggg tatggcgatt acggtcgcgg acccgcccgc ccggaggagg
     9121 gcggttacgc ccccgcggcc ccgccggcgc cggagcaaca acggccgcct taccccgatc
     9181 agggcggcgg ctatgaccag ggctaccagc agggcggggg atacggccag caggactaca
     9241 gccagggcga ctacacccaa tacgccgaaa acgccccggg tggcgggtac gccgccccgc
     9301 aaggcggata cgccgaaacc ggccaccgcg actacggcca accggcggac tacggccagc
     9361 aagccgacta cggccaaccg gcggactacg gccagcaggc gggcggcggc tacggcggtt
     9421 acggccaggg cggctacggc tccgccggga cctcggtcac gctgcagctc gacgatggca
     9481 gcggccggac ctaccagctg cgcgaaggct ccaacatcgt gggtcgtggc caggacgccc
     9541 agttccgcct gcccgacacg ggtgtgtcgc gccgccacct ggagatccgc tgggacggac
     9601 aggtcgcgct gctctccgac ctcaactcca ctaacggcac caccgtcaac aacgcgccgg
     9661 tacaggagtg gcaattggcc gatggcgacg tgatccgttt gggccactcc gagatcatcg
     9721 ttcgcattca ctgaacgcaa cgggatcaaa gctgccgtgc ttcgccccgc gtcgaccgcc
     9781 gtccaagtat cgtgacgttg ctgatgtgct cggtgtcacg ggtgcgacgg agcgccgcca
     9841 ggacggaaag gacgccagat gcagggattg gtactgcagc tgacgcgcgc cggatttcta
     9901 atgttgttat gggtattcat ctggtccgtg ctgcggatcc tgcggacgga tatctacgct
     9961 cccaccggtg ctgtcatggt gcgtcgcggg ttggcgttgc gcggcacact gctgccctcc
    10021 cgccagcgcc gccacgccgc ccgctacctg gtggtgaccg aaggtgcgct tacgggtggg
    10081 cgcatcacgc tcagcgggca gccggtgttg atcgggcgcg ctgacgactc gactctggtg
    10141 ctcaccgatg actacgcctc gacacggcac gcccggctgt cgcagcgcgg ctcggaatgg
    10201 tacgtcgagg atctaggatc gaccaacggc acataccttg acagggcgaa ggtgacgact
    10261 gcggtacggg tccccttagg aacgccgatt cggatcggca aaacggcaat cgagttgcgc
    10321 ccgtgacccg cgccgacgac gatgacgccg gctcggcagg ctatcgagcc ggggtcgtga
    10381 gggtactgcc tgcttgcggg ggagagtggc gcttgtgact ttggtcctgc gatatgccgc
    10441 ccgcagtgat cgcggcctgg tacgcaccaa caacgaggac tcggtctacg ccggcgcacg
    10501 gctcttggcc ctggccgacg gcatgggtgg gcacgctgcc ggcgaggtgg cgtcccagct
    10561 ggtgatcgcc gcgctggccc acctcgacga cgacgagccc ggtggcgacc tgctcgccaa
    10621 gctcgactcg gcggtgcgcg ccggcaactc ggccatcgcc gcacaggtcg agatggagcc
    10681 cgaccttgag gggatgggca ccacacttac cgcaatcttg tttgacggca acaggattgg
    10741 gttggtgcac atcggagact cacgcggcta tctgttgcgc gacggcgagc tggcccagat
    10801 caccaaggac gacacgtttg tccaaaccct ggtcgacgag ggccggatca cacgcgagga
    10861 agcgcacagc cacccgcagc ggtcgttgat catgcgggca ctgaccggtc acgaggtcga
    10921 gccgacgttg accatgcgcg aagcgcgcgc cggtgatcgc tacctgctct gctcggacgg
    10981 gttgtccgac ccggtgagcg acgaaaccat cctcgaggcg ttgcagatcc ccgacgtggc
    11041 cgagagcgcc taccggctca tcgaattggc gctgcgcggc ggcggccccg acaacgtgac
    11101 cgtcgtggtc gccgacgtcg tcgactacga ctacggccag acccagccga ttctggccgg
    11161 cgcggtctcc ggcgaagaag accagatgac gctgcccaat acctccgccg gccgcgcctc
    11221 ggccatcagg ccccgtaacg aggtcgccaa acgcgttcca ccgcaggcgg aaacccccaa
    11281 gcggccacgg tggtcgcggc gccggatggc gatcgttgtc acgctcgtgg tgctgctggt
    11341 gctcgcgggc gtggccatcg gccgtgccat catccgcagc aactactacg tcgccgaata
    11401 caacggcatg gtggccatcc tgcgcggaat tcaaggttcg ctgctgggca tgtcgctgca
    11461 cgagccctac ttgatcggct gcctgaacgc ccgcaacgag ctgtccctgg tcggttccgg
    11521 ccagaccggc ggtcatctcg actgccgctt gatgcaactg aaggatctac gcgcacccga
    11581 acgcgcacag gtccaggccg gtcttccggc cgggaccctg gataacgcga tcgggcagct
    11641 gcgccaactg tcgaccaact cgctgctgcc gccctgtccg ccgcctcggg ccacctcccc
    11701 gcccggatcc ccggtcccgg cggctaccag ctctcccagc ggcacgttac ccaactccac
    11761 ttcgaccggc agtcccgcga ccaccgcgcc ggcagcgccc gcgaccaccg cgccggcagc
    11821 gcccgcgacc acgtcgccca cgaattccca gacggtgact gcgctccccc caccctcact
    11881 tcagccgggc atcgattgcc gggccctgac gtgagcgcgc catgacgaca caagtgcagc
    11941 cgccggtctc ggtgacgccg ccgctgccta ctcggcgcaa cgccgaactg ctgctgctgt
    12001 gcttcgccgc gctgatcacc gtcgctgcac tgctcatcgt cgaggggaac caggagcgca
    12061 gcctgcgctg ggacctgacc agctacgggc tgatcttcct ggtcatcttc ggaagcgcac
    12121 acctggccat ccggcgcttc gcgccgtaca cggacccact cctgctgccg atagtggcgc
    12181 tgctcaacgg gcttggcctg gtgatgatcc accggctcga cctggtcaac aacgagttga
    12241 gcggtcgccg tcaccccagc gcaacccagc agatgttgtg gaccgtggtt ggagtcgccg
    12301 cgttcgcgct ggtagttacc ttcctgaaag accatcggca gcttgcgcgg tacggataca
    12361 tctgtggcgt cgcgggtttg atctttttgg tcattcccgc gctgctgcct gcgtcactgt
    12421 cggagcagaa cggcgccaag atctggatcc gctttcccgg cttctcaatt cagccggccg
    12481 agttttccaa gattttgctg ctgatcttct tttcagcggt gctgatcgcc aaacggcgtc
    12541 tgttcaccag cgtcggcaag cacttcatgg gaatgaccct gccccgcccc cgagacctgg
    12601 ccccgctctt ggccgcttgg gtgatctcgg tgggagtgat gaccttcgag aaggatctcg
    12661 gcacgtcgct gctgctctac gcgtcgtttc tggtggtggt ttacctcgcc acccagcgtt
    12721 tcagctgggt cgtcatcggc ttgctgctgt tcgccgtcgg aagcgtggtg gcgtatttca
    12781 tcttcccgca tgtccgggtt cgggtgcaga tgtggtggga cccgttctcc gaccccgacg
    12841 gcgccggcta tcagatcgtg caatcgctgt tcagcttcgc caccggcggc atcttcggga
    12901 ccgggctggg taacggccaa cccgacaccg tgcccgccgc gtcgaccgac ttcatcatcg
    12961 ccgcgttcgg tgaagagctc gggctggtcg gcctggccag catcttgatg ctctacacga
    13021 tcgtcatcgt ccggggtctg cgcacggcga tcgccacccg cgacagcttc ggcaaactgc
    13081 ttgccgcggg tttggcgtcg acgctggcca tccagctgtt catcgtcgtc ggcggcgtga
    13141 ccaaactcat tccgttgacc gggttgacca cgccgtggat gtcctatggc gggtcgtcgt
    13201 tgctgtccaa ctacgtgctg ctggccatcc tggcccgcat ctcgcacagc gcccgacgtc
    13261 cgttgcgcac ccgcgcacgc aacaagtcgc cgatcgcggc cgccaaaacc gaggtgattg
    13321 agcgggtatg aacgcctctc tccgccggat ctcggtgacc gtgatggcgt tgatcgtgct
    13381 gctgttgctc aacgccacga tgacccaggt cttcgccgcc gacagcctgc gtgccgaccc
    13441 gcgcaaccag cgcgtcctgc tcgacgagta ttcgcgtcag cgcggccaga tcgtcgcgcg
    13501 cgaccagctg ctggcctatt ccgtggccac cgacagccgg tttcgtttcc tgcgggtgta
    13561 tcccaacccc gcggtgtacg caccgatcac cggcttctac tcgctgcgct actccagctc
    13621 gggcctggaa cgggccgagg accccctatt gaacgggtcc gacgagcggc tgttcgggcg
    13681 acggttggcc gacttcttca ccggtcgcga cccgcgcggc ggcaacgtcg acacgacgat
    13741 cgttccgcgg atccaacagg ccgggtggga cgcgatgcag cagggctgtg gcgggccgcc
    13801 ctgcaagggc gccgtcgtcg ccctggaacc gtccaccggc aagatcctgg ccatggtgtc
    13861 gtcgccgtcc tacgacccca acctgctggc gtcgcacgac cccgaggtgc aggcactggc
    13921 ctggcagcgg ctgcgcgacg accccgacaa tccgctgacc aaccgggcca tctcggagac
    13981 ctacccgccg gggtccacgt tcaaggtgat caccaccgcg gcggcgctgc aggccggcgc
    14041 caccgacacc gagcagttga ccgcggcggc ctcgattccg ctgcccggca gcaccgccac
    14101 gctggaaaac tacggtgacg cgccgtgcgg cagcgcgccg acggtgtccc tcagccaggc
    14161 attcgccctg tcttgcaaca ccgcgttcgt ccaactcggc atcctcaccg gtgccgatgc
    14221 gcttcgcaac actgcgcact cgttcgggct ggacaccact cccagcgtga ttccgctgca
    14281 ggtcgccgaa tcgaccgtcg gaataatccc ggacgccgcc gcactcggga tgtcgagcat
    14341 cggtcagaag gacgtcgcac tcacgccgct gcagaacgcg gaaatcgccg cgaccatcgc
    14401 gaacggcggg gtgacgatgc agccgtacct tatcgacagc ctcaaggggc cggacctgtc
    14461 caatatcagc accactgcgc cctatcaaca gcgccgcgcg gtatcgccgc aggtcgccgc
    14521 taagctaaca gagctcatgg tcggcgccga gaaggtcgca caacagaaag gggccattcc
    14581 cggcgtgcag attgcatcca agaccggtac cgcggagcat ggcaccgacc cgcgccacac
    14641 tccaccgcac gcgtggtaca tcgcgttcgc gcccgcgcag acgccgaagg tcgccgtggc
    14701 ggtgctggtg gagaacggtg ccgaccgctt gtccgcgact gggggtgcgc tggctgctcc
    14761 gatcgggcgg gcggtgatcg aggccgcgct acagggggga ccatgagccc gcgagttggg
    14821 gtgacgctgt caggcaggta tcgcctgcaa cgtctgatcg ccaccggcgg catgggccaa
    14881 gtgtgggaag cggtggacag ccggttgggc cggcgggttg cggtcaaggt gctcaaacag
    14941 gagttctccc aggaccccga gttcattgaa cgtttccgcg ccgaggcccg caccaccgcc
    15001 atgctcaacc accccgggat cgccgccgtc cacgactacg gcgaaagcca gctggacggc
    15061 gagggccgaa ccgcctacct ggtgatggag ctggtcaacg gcgaaccgct gaattcggtg
    15121 atcaagcgca ccggccggct ctcgctgcgg catgcgctgg acatgctcga acagacaggc
    15181 cgggcccttc aagtggcgca cgctgccggc ttggtacacc gcgacgtcaa accgggcaac
    15241 atcctgatca ccccgaccgg gcaggtgaag atcaccgact tcggcatcgc caaggccgtc
    15301 gacgcggcgc cggtgaccca gaccgggatg gtgatgggca ccgcccaata catcgccccc
    15361 gagcaggcgc tgggccacga cgccaccccg gccagcgatg tctattcgtt gggtgtcgtc
    15421 gggtacgagg tggtttcggg caagcggccc ttcaccggcg acggggcttt gacggtggcg
    15481 atgaaacaca tcaaggaacc cccgccgccg ctgcctcccg agctgccgcc caatgtgcgc
    15541 gaactcatcg agatcacctt ggtgaagaac cccaccatgc ggtatcgcag cggcgggccg
    15601 ttcgccgacg cggtcgccgc ggttcggtcc ggccgacggc cgccgcggcc gaaccaggcg
    15661 ccgtccatcg gcagagcggc accagcagca gtgccatcgg ccgcacaggc gcgtgccgcc
    15721 gcagatct
//
LOCUS       DASSGQ010000108         8213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1010263,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000108 DASSGQ010000000
VERSION     DASSGQ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8213
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010263"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..870
                     /locus_tag="VFI55_05010"
     CDS             166..870
                     /locus_tag="VFI55_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7073426.1"
                     /translation="MVVMPGASSVRPYRGVAATQRLAARRGRLLAAGLDLLGAEQQDI
                     SELTVRGICGQAGLAARYFYESFADKDEFVSGVFDWVIVELATTTQAAVAAVPVPEQS
                     RAGIANIVRTIDGDARIGRLLFSTQLANAVVMRKRAESSALFAMLSGKYAGDALRMPA
                     NDRIKAGAHFAVGGVGQTISAWLAGDVRLEPPQLVDQLAALLDELTDPKLYGLKETAA
                     GATAASPDPAATNATS"
     gene            complement(803..1351)
                     /locus_tag="VFI55_05015"
     CDS             complement(803..1351)
                     /locus_tag="VFI55_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014943072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073427.1"
                     /translation="MTDGILTDAQIAALKPAERRELVQRLEQPLKDVIDPVLLTRIRR
                     IRLSLMIGCSIAMIPWIVYLSLTLPENYVAHNWPLTWVGFDVLLVGFMVATAVFGYLR
                     RQVLLLAAFTSGVLLICDAWFDLMTAGPDDVWLSVVTALFIEVPLAIFMIRGAQRIMR
                     LTMLRLWLLDPGMRLWHLPLFP"
     gene            complement(1387..1968)
                     /locus_tag="VFI55_05020"
     CDS             complement(1387..1968)
                     /locus_tag="VFI55_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7073428.1"
                     /translation="MIKPHYANTEFELGGINHVALVCSDMERTVDFYTNVLGMPLIKS
                     LDLPGGAGQHFFFDAGNGDCVAFFWFADAPDRVPGVSSPGAIPGIGDITSAVSTMNHL
                     AFHVPAEKFDAYRQRLKDKGVRVGPVLNHDESEMQVAATLHPGVYVRSFYFQDPDGIT
                     LEFACWVKEFDASDTKAVPRTAADRRPPVATAR"
     gene            2066..2716
                     /locus_tag="VFI55_05025"
     CDS             2066..2716
                     /locus_tag="VFI55_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7073429.1"
                     /translation="MSTAHQPTERPAEFPTHRGRRTQAALDAAARTVIARKGVLATTV
                     ADIAAEAGRSSASFYNYYDSKEAMVRQWALRFRDDADQRARSVTRHGLSNRERAHEAA
                     TAHWQTYRSRLAEVISVSQLAMVNDDFARYWAEICAIPVSFIAETVRRAQAEGHCPDD
                     DPELIAQAIVAMFNQFCYIQLTGTSSAATEPDDAACIKTLANIYYRAIYAGEGESN"
     gene            2713..3846
                     /locus_tag="VFI55_05030"
     CDS             2713..3846
                     /locus_tag="VFI55_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7073430.1"
                     /translation="MNRHTGAAGVVREFVGLPSPTAGRAGAGGHPCQGIYHRGVGRKP
                     KVAMIAAHYQIDFSEHYLADYMATRGIGFLGWNTRFRGFESSFLLDHALVDIGVGVRW
                     LREVAGVDTVVLLGNSGGGSLMAAYQSQAVEPNVTPLQGMRPAAGLTDLTAADGYVAT
                     AAHPGRPDVLTAWMDAAVVDENDPVATDPQLDLFNEDNGPPYPAEFIARYRRAQTARN
                     QAITDWAETELKRVRAAGFSDRPFTVMRTWADPRMVDPGIEPTKRPPNTCYAGVPAKA
                     NRSAHGIAAACTLRNWLGMWSLRVAQTRAEPHLARITCPALVINAGADTGVFPSDAER
                     IHDGLAGTDKAQVSIDSDHYFTTPGARSELADTIVKWIGKRWR"
     gene            3822..4793
                     /locus_tag="VFI55_05035"
     CDS             3822..4793
                     /locus_tag="VFI55_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013827205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HET7073431.1"
                     /translation="MDRQAVALRVLAHFTPGDKVLDFVAPESDWLDVRWCADNDDTRF
                     YRELPDAEVIWHSLRPLSGDDLRRGARLRLVQKLGIGVNTIDVETATKCGIAVANMPG
                     ANAPTVAEGTVMLMLAALRRLLALDRAVRHGSGWPADPELGETVRDIGSCTVGLVGYG
                     NIAKRVGDIVAAMGASVLHNTTRDDGRPGWRQLPELLAACDIVSLHVPLTTETHHLLS
                     RDALARMKPTAVLVNTARGAIVDEEALVDALRGGRLAAAGLDVFEVEPVAPDNPLLGL
                     DKVVLTPHVAGFSADSMRRYLTEAVANCRRLRDGEPLANVVNQPTGC"
     gene            4848..7049
                     /locus_tag="VFI55_05040"
     CDS             4848..7049
                     /locus_tag="VFI55_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET7073432.1"
                     /translation="MPIAITPEHHDLADSVRSLVARVAPSEVLHAALETALENPPPYW
                     HAAAEQGLQGVHLAESVGGQGFGILELAIVLAEFGYGAVPGPFVPSAIASALISAHDA
                     QAKVLFELASGAAIAAYALDSGLTATRQGHDEKTLVIRGEVRAVPAAAQASVLVLPVA
                     IDGGEEWVVLRAEQLEIEPVKSIDPLRPIAHVRANAVEVGDDAALGNLTMTTAHALIS
                     TLLSAEAVGVARWATDTASHYAKIREQFGRPIGQFQAIKHRCAEMIADTERATAAVWD
                     AARAIDEAGQHDSDIVGVEFAAAVAATLAPAAAQRCTQDCIQVHGGIGFTWEHDTNIY
                     YRRALMLAASFGRRSEYPQKVVDTATGTGMRAVNIDLDPDTEKLRTEIRGEVAAFKAM
                     DRESRKVAIAEAGLVLPYLPEPWGRAASPVEQVIIAQEFTTGRVKRPQVGIAAWIIPS
                     IVAFGTEDQKQRFLPPTFRGEMIWCQLFSEPGAGSDLAGLSTKATRTDGGWRITGQKI
                     WTTAAQYSQWGALLARTDPSAPKHNGITYFLLDMKSEGITVTPLRELTGNAMFNTVYI
                     DDVFVPDEFVLGEVNRGWEVSRNTLTAERVSIGSSDANFLATLPEFVEFVRDGQLDQI
                     AQHRAGHLIAEGHAAKVLNLRSTLLTLAGGDAMPSAAISKLLSMRTGQGYAEFAVSSF
                     GTDAAIGDTDEPAGKWGEYLLASRATTIYGGTSEVQLNIIAERLLGLPRDP"
     gene            complement(7219..7701)
                     /locus_tag="VFI55_05045"
     CDS             complement(7219..7701)
                     /locus_tag="VFI55_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073433.1"
                     /translation="MMISPRSVRSAAAGAVGAGVLTGALLFGGAPAAQAAPPAAAGTS
                     FEMAGPHGAPDLPLRPGGGGGGHGGGGHGGGGWGGHGGGGWGGHGGWGHGGWGNGGWG
                     HGGWGHGGWGNGGWGNGGWGRGGWGHGGWGGHPGWGNGGWGPPGLGFRPWWNLWNWWW
                     "
BASE COUNT         1462 a         2775 c         2684 g         1292 t
ORIGIN      
        1 gttggcggct ccgcgatcgc ccgttcgaca tgccgaatcg gctggtgcac cttgtgaatg
       61 gccatagcaa gtcctccggt ggcgcggcgg gctaatgtgc gaacgaatgt ttcctgatgt
      121 tgtcttggtc tgatgtcggt gtcaagacgg tcgttcggtg tcaagatggt tgttatgccc
      181 ggtgcttcca gcgtgcgccc ctaccgcggc gtcgctgcca cacaacggct ggccgcgcgc
      241 cgcggccggc tactggccgc cgggctggac ctgctgggcg ccgagcaaca ggacatctcc
      301 gagctgaccg tccgcgggat atgcggccag gccggcctgg cggcccgcta tttctacgaa
      361 agtttcgccg acaaagacga attcgtcagt ggagtgttcg actgggtgat cgtcgagctg
      421 gccaccacca cccaggccgc ggtggcggcg gtcccggtgc ccgagcagag ccgcgcgggg
      481 atcgccaaca tcgtgcggac catcgacggt gacgcccgca tcggacgact gctgttcagc
      541 acgcaactgg ccaacgccgt ggtcatgcgc aagcgcgccg aatccagcgc cctgttcgcc
      601 atgctgtcgg gcaagtacgc cggcgatgcg ctgcggatgc cggcaaacga ccgcatcaag
      661 gccggggccc acttcgcggt cggcggcgtc ggtcaaacca tcagcgcctg gctggccggc
      721 gacgtccgac tcgagccacc gcagcttgtc gatcagctgg ctgcgctgct ggacgaactc
      781 accgacccaa aactgtacgg gctcaaggaa acagcggcag gtgccacagc cgcatccccg
      841 gatccagcag ccacaaacgc aacatcgtga gtcgcatgat gcgctgcgcg ccgcggatca
      901 tgaagatggc caacggcacc tcgatgaaca gcgcggtgac caccgacagc cacacatcgt
      961 caggtccggc cgtcatcagg tcgaaccacg cgtcgcagat cagcagcacc ccggaggtga
     1021 aggccgcgag cagcagcacc tggcggcgca ggtatccgaa cacggccgtt gccaccatga
     1081 atccgaccag caggacgtcg aagcccaccc aggtcagcgg ccagttgtgg gccacgtagt
     1141 tctccggcag cgtcagcgag aggtacacga tccacggaat catcgcgatg gagcagccga
     1201 tcatgaggct caggcgaatg cgccgtatcc gggtgaggag caccgggtcg atcacgtcct
     1261 tgaggggttg ctccagccgc tgaaccagct cccgccgctc cgccggcttc agtgccgcga
     1321 tctgcgcgtc ggtcagaatg ccgtcggtca tcatggggtc ccccgagatc gggtgcggtc
     1381 tgccggctag cgtgcggtgg ccaccggagg tcgccggtcg gccgcggtcc tgggcacggc
     1441 cttcgtgtcg ctcgcgtcga attccttgac ccagcaggcg aattccaacg tgatgccgtc
     1501 ggggtcctgg aagtagaacg agcgcacgta cacgcccgga tgcaacgttg cggcgacctg
     1561 catctcgctc tcgtcgtggt tgagcaccgg gcccacccgc acgcccttgt ccttgagccg
     1621 ctgccggtag gcgtcgaact tctcggccgg cacatggaac gccagatggt tcatggtgct
     1681 caccgcgctg gtgatgtccc cgatgcccgg gatggcgccc ggcgacgaga cgcccggcac
     1741 tcggtccggc gcgtcggcga accagaaaaa ggcgacgcag tcgccgttgc cggcgtcgaa
     1801 gaagaagtgc tgccccgccc cgccgggcag atccagcgat ttgatcagcg gcatgccgag
     1861 aacgttggtg tagaagtcca cggtgcgctc catgtctgaa cacaccagcg cgacgtggtt
     1921 gatcccgccg agctcgaact ccgtgttggc gtagtgcggc ttgatcatct tgcggctccc
     1981 atcggtccgc ggggcgcggg tcctggccaa gatacgaatc tgaatctaac atcaggttca
     2041 gttttgctcc agggaggtac gccacgtgtc gaccgcacac cagccgaccg agcgtcccgc
     2101 cgaatttccg acgcatcgcg gccgccgcac gcaggcggcg ctcgacgcgg ccgcgcgcac
     2161 ggtcatcgca cgcaagggcg tcctggcgac caccgttgcc gacatcgccg ccgaagcggg
     2221 ccgctcgtcg gcctcgtttt acaactacta cgactccaaa gaggcaatgg tccgccaatg
     2281 ggccctgcga ttccgcgacg atgccgatca acgcgcgcgt tcggtgaccc ggcacggcct
     2341 gtccaaccgc gaacgcgccc acgaggcggc gacggcccac tggcagacct accgcagtcg
     2401 gctcgccgag gtgatcagcg tgtctcagct ggcgatggtc aacgacgact tcgcgcggta
     2461 ctgggccgag atatgcgcga tccccgtctc tttcatcgcc gagacggtcc ggcgcgccca
     2521 ggccgagggc cactgccccg acgacgaccc cgagctgatc gcgcaggcga tcgtggccat
     2581 gttcaaccag ttctgctaca tccagctcac cggaacaagt tcggccgcaa ccgaacccga
     2641 cgacgcggcg tgcatcaaga cgctcgccaa catctactac cgagccatct acgccggtga
     2701 aggggagtcg aattgaatcg tcatacgggt gctgcagggg ttgtccggga gttcgtaggg
     2761 ttgccgtcgc ccactgccgg gcgcgccggg gcgggagggc atccctgcca gggcatctac
     2821 caccgggggg tgggacgcaa gccgaaggtg gcgatgatcg ccgcgcacta tcagatcgac
     2881 ttctccgagc attatctggc cgattacatg gccacccgcg gcatcggatt tctcggctgg
     2941 aacacgcggt tccgtggttt cgaaagcagc tttctgctcg accacgcgct ggtcgacatc
     3001 ggcgtcgggg ttcgttggct gcgtgaggtc gccggcgtgg acaccgttgt cctgctcggc
     3061 aattcggggg gaggctcgtt gatggcggcc tatcagtcgc aggccgtcga accgaatgtg
     3121 acgccactgc agggcatgcg tccagcggcc gggctgaccg acttgaccgc cgccgacggc
     3181 tacgtcgcga ccgccgctca tcccgggcgc ccggacgtgc tgacggcctg gatggacgcc
     3241 gccgtcgtcg acgaaaacga cccggtggcc accgatcccc agctcgatct gttcaacgag
     3301 gacaacggcc cgccgtaccc ggcggagttc atcgcccgct accgccgcgc gcagaccgcg
     3361 cgcaaccagg ccatcaccga ctgggccgaa accgagctca aacgtgttcg cgccgcaggg
     3421 ttttcggatc gaccgttcac cgtgatgcga acctgggccg acccccgcat ggtcgatccc
     3481 ggcatcgagc ccacaaagcg accgccgaac acgtgttatg cgggcgtccc cgccaaggcg
     3541 aaccgttcgg cacacggcat cgcggcggcc tgcacgctgc gcaactggct gggcatgtgg
     3601 agcctgcggg tggcacagac ccgggccgag ccgcacctgg cccgcatcac ctgccccgcg
     3661 ctggtgatca acgccggggc cgacacggga gtatttccct ccgacgccga gcgcattcac
     3721 gacgggctcg ccggcaccga caaggcacag gtctcgatcg acagcgacca ctacttcacc
     3781 accccgggcg cgcgcagcga gctggccgat accatcgtca aatggatcgg caagcggtgg
     3841 cgctgagggt tctcgcccac ttcactcccg gcgataaggt tctcgatttc gttgcgccgg
     3901 aatcggattg gctcgacgta cgatggtgcg ccgacaacga cgacacgcgc ttctaccggg
     3961 aattgcccga cgcggaggtg atctggcact cacttcggcc gctgtcgggc gacgacctgc
     4021 ggcgcggggc gcggctgcgg ctggtgcaga aactcggcat cggggtgaac accatcgacg
     4081 tggaaaccgc aacgaagtgc ggtatcgcgg tcgccaacat gcccggcgcc aacgcaccga
     4141 cggtggccga gggcacggtg atgctgatgc tggccgcctt gcgccggctg ctggcgctgg
     4201 accgcgcggt gcggcacgga tccggctggc cggcggatcc ggaactgggc gagacggtgc
     4261 gcgatatcgg cagctgcacc gtcggcctgg tcggctacgg caacatcgcc aaacgggtgg
     4321 gcgacatcgt cgccgcaatg ggcgccagcg tcctgcacaa caccacccgt gacgacggac
     4381 gcccgggttg gcggcagctg cccgaactgc tggccgcctg cgacatcgtc tcgctacacg
     4441 tgccgttgac gacagagacc catcatctgc tcagcagaga cgcgctggcc cggatgaaac
     4501 caaccgcggt gctggtcaac acggcacgtg gcgccatcgt cgacgaagag gcactcgtcg
     4561 acgcgctgcg aggtgggcgg ctggctgccg cgggtttgga cgttttcgag gtcgagccgg
     4621 ttgctcccga caatccgctg ctcggcctcg acaaggtggt gctgacaccg cacgtcgccg
     4681 ggttcagcgc cgacagcatg cggcggtatc tgactgaggc cgttgccaac tgccgccggc
     4741 tgcgcgacgg cgaaccgctt gccaatgtgg tcaaccaacc gaccggttgt tagcttggaa
     4801 ggtgcttggc cgaaacggac tgcgaaagcc acgggaaggc gcgaccaatg ccgatcgcaa
     4861 taactcctga gcatcacgac ctggccgact cggtgcgatc cctggtggcg cgggtagcac
     4921 cgtccgaggt actgcacgcg gccttggaaa cagcactgga gaacccgccg ccgtactggc
     4981 atgccgccgc agagcaaggg ctgcaggggg tgcatctggc ggaatccgtc ggcgggcaag
     5041 ggttcggcat cctcgaattg gccatcgtgc tggccgagtt cggctacggg gcggtgcccg
     5101 gaccgttcgt cccgtcggcg atcgccagcg cgctgatctc cgcgcacgac gcgcaagcga
     5161 aggtgctctt cgaattggcg tccggcgccg cgatcgccgc ctacgcgctg gattccggcc
     5221 tgaccgccac ccggcagggc cacgacgaga agacgctggt gatccgcggc gaggtccgcg
     5281 cggttcccgc cgcagcgcag gcatcggtgc tggtgctgcc cgtcgcgatc gacggcggcg
     5341 aagaatgggt ggtgctgcgc gccgaacagc tcgagatcga gccggtgaaa agcatcgacc
     5401 cgctgcgtcc catcgcgcat gtgcgggcca acgcggtcga ggtcggcgac gatgccgcgc
     5461 tgggcaatct gaccatgacc accgcgcacg cgctgatttc gacgctgctg tcggccgagg
     5521 ccgtcggcgt ggcgcgctgg gccaccgaca cggcgtcgca ctacgccaag atccgcgagc
     5581 agttcggcag gccgatcggc cagttccagg ccatcaagca caggtgcgcg gagatgatcg
     5641 ccgacaccga gcgagccacc gcggcggtgt gggatgcggc ccgcgcgatc gacgaggccg
     5701 gccaacacga ttcggacatc gtcggagtcg agttcgccgc ggcggtggcc gcaaccctgg
     5761 cgccggcggc cgcccaacgg tgcactcagg attgcatcca ggtgcacggc ggcatcggct
     5821 tcacgtggga acacgacacg aacatctact accgccgggc gttgatgctg gcggcctcct
     5881 tcggccgcag atcggagtac ccgcaaaagg tggtggacac cgccaccggc accgggatgc
     5941 gggcggtgaa catcgatttg gatcccgaca ccgaaaaact gaggaccgag atccggggcg
     6001 aggtcgccgc gttcaaggcg atggaccgcg agtcccgcaa ggtcgcgatc gccgaggccg
     6061 ggttggtgct gccgtacctg ccggagccct ggggccgggc cgcgagtccg gtggagcagg
     6121 tcatcatcgc ccaggagttc accacgggac gcgtcaagcg gccccaggtc ggcatcgcgg
     6181 cgtggatcat cccgtcgatc gtggcgttcg gcaccgagga ccagaagcaa cggttcctgc
     6241 cgccgacctt ccgcggcgaa atgatctggt gtcagctgtt ttccgagccg ggcgccggat
     6301 cggatctggc ggggctgagc accaaagcga ccaggaccga cggcgggtgg cgcatcaccg
     6361 ggcaaaagat ctggacgacc gcggcgcagt attcgcagtg gggcgcgcta ttggcgcgga
     6421 cggacccgag cgcgccaaaa cataacggca tcacgtactt cctgctggac atgaagagcg
     6481 aaggcattac ggtgacgccg ctgcgcgagc tcaccggcaa cgcgatgttc aacacggtct
     6541 acatcgacga cgtgttcgtg cccgacgagt tcgtgctcgg cgaggtcaac cggggctggg
     6601 aggtcagtcg caacaccctg acggcggagc gggtgtcgat tggcagcagc gatgcgaatt
     6661 tcctggcgac actgcccgag ttcgtcgagt tcgtccgaga cgggcagttg gaccagatcg
     6721 cgcaacaccg ggccgggcac ctgatcgccg aaggccatgc cgccaaggtg ctgaacctgc
     6781 gctcgacgct gttgacgctg gccggcggtg acgcgatgcc gtcggcggcg atctccaagc
     6841 tgttgtcgat gcgcaccggc cagggctatg ccgaattcgc cgtgtcctcg ttcgggaccg
     6901 acgccgcgat cggcgacacc gacgagccgg cgggcaagtg gggcgaatac ctgctggcca
     6961 gccgggccac cacgatttat ggcggtacgt ccgaggtgca gctcaacatc atcgccgaac
     7021 ggctgttggg cctgccgcgc gacccctgaa cacaccatgc cgacctgagg cccgtctcag
     7081 gtcggcatgg acattcctca agggcgggcc atgccgacct gcgggtggca ggccggcatg
     7141 gattcccccc acagcgtggg cccggtcaac tcggtagcga cgtcactgac cagtgccaag
     7201 cggtgattgc ggcttgactt accaccacca gttccacaag ttccaccacg gccggaagcc
     7261 caaaccgggg ggcccccaac caccattgcc ccaaccaggg tggccccccc aaccgccgtg
     7321 gccccagcca ccgcgacccc agccgccatt gccccagccg ccattgcccc agccgccgtg
     7381 gccccagccg ccgtggcccc agccaccatt gccccagccg ccgtggcccc agccaccgtg
     7441 gccgccccag ccgccaccgc cgtgaccgcc ccagccgcca ccgccgtgac ccccgccgcc
     7501 atggcccccg ccgccgccac cgggcctaag cgggaggtca ggagcgccgt gggggccggc
     7561 catctcgaag ctcgttccgg cagcggctgg tggagcggct tgcgcggcgg gggcgccgcc
     7621 gaacagcagc gctccggtca gcacgccggc gccgaccgca ccggcggccg cgctgcgcac
     7681 ggatcgcggt gaaatcatca ttgcgagctc cttccaagac ctgtactacg cctaaaacgt
     7741 tactcagaat ttcataggca aacaatagct atagcgcgaa aattgctgtg aattagttga
     7801 cagtcgctaa cgctagttat caggcattcg caccaaatca tgtatttctt aatatccatt
     7861 tacgcaatta agtatttacg cacttcagca cttgcgcagc tactgaagac caggccgtcg
     7921 ccacggctaa taacagcagc tcatgctcca tatactgaac tactgaagta cgtacttccg
     7981 cttctcagta cctcatgatc taccacttta cgcagtccga catgaatcgc gacgattagg
     8041 ccactgttag ggaactgtta aacgatctgc aaatactttc atagaggcca tgaactgacg
     8101 cttcgcaacg atatgccttt ctacagctat ctcctggaaa ggtgtggcga acgcgctggt
     8161 agacggcttg gcgcgcaccc gaccgccgtc ggtcgcctac tgccgactcg cta
//
LOCUS       DASSGQ010000109        12659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1070773,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000109 DASSGQ010000000
VERSION     DASSGQ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12659
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070773"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="VFI55_05050"
     CDS             <1..1449
                     /locus_tag="VFI55_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014712296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7073434.1"
                     /translation="SEYASLRTRIGMVPQDDVVHRQLTVNQALRYAAELRLPPDTSRA
                     DRHQVVAQVLDELGLTEHADTRVDKLSGGQRKRASVALELLTGPSLLILDEPTSGLDP
                     ALDLQVMTMLRQLADAGRVVLVVTHSLTYLDVCDQVLLMAPGGKTAFLGPPGQIGDAM
                     GTTNWARIFAKVGADPDEANRRFLARNQPPPPVETAPADLGAPAHTSLRQQFSTIARR
                     QVRLVVSDRAYFTFLALLPFILGALSLTVPGHAGFGVAEPTSSTPDEAAQILILMSIA
                     AVFMGTALTIRDLIGERAIFRREQAVGLSTGAYLTAKIAVYCVFAIVQAAITTGIVLV
                     GKGAPTQPAVLLGPSTFAAGVELFVTVAATCVASAILGLALSSIARSNEQIMPLLVVS
                     LMLQLVLAGGIVPVTGRMVLDQMSWTVPSRWGYAASASTVDLRALVPGSLIPKDRHWL
                     HTRGAWLLDMGMLAALSLVYAVIVRWRIRLKR"
     gene            complement(1453..2022)
                     /locus_tag="VFI55_05055"
     CDS             complement(1453..2022)
                     /locus_tag="VFI55_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010839295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HET7073435.1"
                     /translation="MPPGSDWSDCHFEGRDFRDEDLSRLRTERAVFTECNFSGVNLAE
                     SQHRGSAFRNCTFERSLLWHATFAQCSMLGSVFVQCRLRPITFDEVDFTLAVLGGNDL
                     RNVDLSGCRLRETSLVETDLRKAVLRGADLSGARTTGTRLDDADLRGATVDPSLWRTA
                     SLAGACVDVGQALAFALAHGLRFEGSMDG"
     gene            complement(2028..2600)
                     /locus_tag="VFI55_05060"
     CDS             complement(2028..2600)
                     /locus_tag="VFI55_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HET7073436.1"
                     /translation="MALKHSDRGHASTKIVVAGGFGVGKTTFVGAVSEIMPLRTEAML
                     TDASAGVDMLETTPDKRTTTVAMDFGRITLDEDLVLYLFGTPGQRRFWFMWDDLVRGA
                     IGAIVLVDCRRLQDSFAAVDFFEHRNLPFLVAVNEFDGAPRYPVHEVREALTLAAHIP
                     VITVDARDRRSATDALIAVSEYALEALAAS"
     gene            complement(2581..2955)
                     /locus_tag="VFI55_05065"
     CDS             complement(2581..2955)
                     /locus_tag="VFI55_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
                     /protein_id="HET7073437.1"
                     /translation="MDTPDTWPPSPEANLVRPYTLTAGRTDTDVDLPLEAPIQTLEAG
                     LAHRWPPDDVRGRIIQLCVDSPSVAEISARLDLPLGVVRVLVGDLVLSGYLRVHRTLT
                     ERSTRDERHELIGRTLRGLKAL"
     gene            complement(2962..3372)
                     /locus_tag="VFI55_05070"
     CDS             complement(2962..3372)
                     /locus_tag="VFI55_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET7073438.1"
                     /translation="MTSAGNSLDWLVTKFAREVPGVAHALVVSVDGLPVAASEHLPRE
                     RADQLAAVASGLASLASGAAQLFEGGQVLQSVVEMQNGYLLLMRVGDGSHLATLAATS
                     CDIGQIGYEMAILVERVGSMVESSRRSAPSSAHS"
     gene            complement(3369..6215)
                     /locus_tag="VFI55_05075"
     CDS             complement(3369..6215)
                     /locus_tag="VFI55_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7073439.1"
                     /translation="MTMFTRPAHSVEAALPEPTPIPAAEATPAKRPPAWSPSNWPVRS
                     KVLAIVLVPLVLATAFGALRVKDAMAGSRNLNLAADRAEVLPAITKYMSALDVALLAS
                     STGRDVDGAKKNYDARKYELQTRLADTDVTPDVRSGVSTLINGGQGLLDKVADNSIGL
                     RDRVTTYAPILLTAEDVINASVRVGDEQIRAEAQGLSRAVGARGQMTMQEILVTRGAD
                     LPEPQLRTSMITLAGTEPSTLFGMSEVLGASSPEAKTLQQQMVNRMAIMSDPASVLVD
                     NPELLRSIQTTDTIAEQVTDDATASVTKSVHAEAADRRNAAIRDTVLVLSAVVIALAI
                     VLVMARTLVRPLRVLRDGALRVAHTDLEEEVARVRAGGAEPILQPLPVYTTEEIGQVA
                     HAVDELHTQALLLAGDEARLRLLVNDMFETMSRRSRSLVDQQLALIDRLERDEEDPDR
                     LDNLFRLDHLAARLRRNSANLLVLAGAQLARDQREPVPLATVINAAVSEVEDYRRVEI
                     TGLPDCALIGSVAGGVIHLFAELIDNALRYSPPTTSVQVSAARGGDGGVVLRISDSGL
                     GMNDTDRRIANMRLRAGGEVTPDPTPDNARHMGLFVVGRIAARHDIRVGLRGPAANDA
                     GSGTTAEIYLPPTALEGVSVAQAPEPRTAGADPPPSVADVAAPQTANRPHHPNPPNHS
                     DHPNHQGFTEPGPSVTLLPRRNPGSSGITDVPAAPAEQQPSPRRRELATPWWETRQQT
                     KQSPAAPRTAAASDTSAFLAARSQSAADRAEAPPVRPASPAAAANISKPSIPVVPPAA
                     TASTAPADDDVIYRRMLSEMLGDPHDLVNSPDLDWQSVWDRGWSLAAAAEDKPVESHT
                     AEHGLPVRKPGARLVPGAANGMSLTGQDPQDQQDQSQGLLVTSNGAHSGRQPQHSSAT
                     RDPEAVRASFSSHFGGVRSARSHARDASEGSDQQ"
     gene            6480..6944
                     /locus_tag="VFI55_05080"
     CDS             6480..6944
                     /locus_tag="VFI55_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET7073440.1"
                     /translation="MFRLLFYSPRIAPNTGNAIRTVAATGAELHLVEPLGFDLSEPKL
                     RRAGLDYHDLASVTVHPSLPAAWDALSPQRVIAFTAHATTSFADVDYHAGDVLMFGPE
                     PDGLDAATLADAHITGQVRIPMLAGRRSLNLSNAAAIAVYEAWRQHGYAGAI"
     gene            complement(7018..7662)
                     /locus_tag="VFI55_05085"
     CDS             complement(7018..7662)
                     /locus_tag="VFI55_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET7073441.1"
                     /translation="MTLNLSFDEVLTTTRSVRKRLDFDKPVGRDVLMECLELALQAPT
                     GSNSQGWQWVFVEDAEKKKAIGDIYLANARTYLSSPSPEYADGDTRGERMGKVKDSAT
                     YLAEHMHEAPVLMIPCIYGREDKQPLGGASFWASLFPAVWSFCLALRSRGLGSCWTTL
                     HLLAGGEKKAAEILGIPDDRYSQAGLFPIAYTKGTDFRPAKRLPAESLTHWNTW"
     gene            complement(7699..11019)
                     /locus_tag="VFI55_05090"
     CDS             complement(7699..11019)
                     /locus_tag="VFI55_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HET7073442.1"
                     /translation="MGWFNGPPSWAEMERVLDGKPRHAGAPAAEPAFDPGGDVPLSHK
                     RGTYQPPSSGRAARSSVAHSVPYAELHAHSAYSFLDGASTPEELVEEAARLGLRALAL
                     TDHNGLYGAVRFAEAATELDVRTVFGAELSLGSVARTEQPDPPGPHLLVLARGPEGYR
                     RLSRQLAAAHLAGGEKGKPRYHIDALTEAAGGHWHILTGCRKGTVRHALSDGGPDAAA
                     RALADLVDRFGAGRVSIELTHHGHPLDDEHNATLAGLAPRFGVGVVATTGAHFAHPSR
                     SRLAMAMGSIRARQSLDSAAGWLAPLGGSHLRSGEEMARLFAHVPLGGPDVVAAAAEL
                     GEQCAFGLALIAPRLPPFDVPDGHTEDSWLRQLTMAGARDRYGLPDGASRAYSQIERE
                     LKVIAQLTFPGYFLVVHDIARFCRENNILCQGRGSAANSAVCYALGVTAVDPVANELL
                     FERFLSPARDGPPDIDMDIESDQREKVIQYVYDKYGRDYAAQVANVITYRGRIAVRDM
                     ARALGFSQGQQDAWSKQISHWNGLADSPDLDGIPLQVIDLATQIRNLPRHMGIHSGGM
                     VICDRPIADVCPVEWARMENRSVLQWDKDDCAAIGLVKFDLLGLGMLSALHYVIDLVA
                     EHKGIEVDLARLDLSEPAVYEMLCRADSVGVFQVESRAQMATLPRLRPRVFYDLVVEV
                     ALIRPGPIQGGSVHPYIRRRNGIDPVVYDHPSMESALRKTLGVPLFQEQLMQLAVDCA
                     GFSAAEADQLRRAMGSKRSTERMRRLRGRFYDGMRALHGATDEVIDRTYEKLEAFANF
                     GFPESHALSFASLVFYSSWFKLHHPAAFCAALLRAQPMGFYSPQSLVADARRHGVTVH
                     GPDVNASLAHATLEDAGNTVRLGLGAVRHIGDDLAEQLVEERKTNGPFASLLDLTSRL
                     QLSVPQTEALATAGALGCFGMSRREALWAAGAAATQRPDRLPGVGSSSHIPPLPGMSE
                     LELAAADVWATGVSPDSYPTQFLRADLDAMGVVPAEALLSVPDGDRVLIAGAVTHRQR
                     PATAQGVTFINLEDETGMVNVLCTQGVWARHRKLANTAPALLIRGQVQNASGAVTVVA
                     ERLGRISLVVGSKSRDFR"
     gene            11170..12549
                     /locus_tag="VFI55_05095"
     CDS             11170..12549
                     /locus_tag="VFI55_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015303852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HET7073443.1"
                     /translation="MAELTTLDTGPLGTQDPDRQVSLAIGAVAVVDGAAPDHDLLKRV
                     LEERIQSIPRCTQVLRTHPFNGAQQWVDDPRFDFDHHVRRVTIPRPGDEAELSRAIAQ
                     ALERPLDPDRPLWECWVIEGLKGNRWAILMKIHHCLSEGNSAAHLLARLCDEADTDIF
                     VNHVGSKHVSPSQTPKRSLFDALWRASTIAGNVTNTLAGSIWSAVRSPSTIPVNTRRR
                     YRTVRVPLTAVDDVCRKFGVTTNDVALAAITEGFRTVLLHRGEQPRADSLRTLDKTDN
                     RISAMLPYLPVEHDDPVQRLQTVHSRLNRPKQTSQRPTPAIVRLANSYPPFVLSAKLA
                     KLLLSRLPQPGIVTLATNAPGPRHRLGLMGRTVERLLPIPPTASQLSSGVAVLSYGDE
                     LVFGITADYDNAAELEQLAVGIERGMARLVALSQDSVLLFTKESRKRPARALPTAAQR
                     GRPSAPGRP"
BASE COUNT         2005 a         4377 c         4225 g         2052 t
ORIGIN      
        1 agcgagtacg cctcgttgcg gacccggatc ggcatggttc cccaagacga cgtcgtgcac
       61 cggcagctga cggtcaacca ggcgctccgc tacgcggccg agctgcggct gccgcccgac
      121 accagcaggg ccgaccggca ccaggtcgtc gcccaggtgc tcgacgaact tggactgacc
      181 gagcacgcgg acacccgcgt cgacaagctg tccggcggcc agcgcaaacg cgcctcggtg
      241 gcactggagt tgctcaccgg gccgtcgctg ctgatcctcg acgaaccgac gtcgggtctg
      301 gacccggcgc tggacctgca agtcatgacg atgctgcggc agctggccga cgccggtcgc
      361 gtggtgctgg tcgtcaccca ctcgctgacc tatctcgacg tctgtgacca ggtgctgctg
      421 atggcacccg gcggcaagac ggcgtttctg ggcccacccg gccagatcgg cgacgcgatg
      481 gggaccacca actgggcgcg gattttcgcc aaggtgggcg ccgatcccga cgaggccaac
      541 cgccgctttc tggcccgcaa ccagcccccg ccgcccgtcg agacggcacc ggccgacctg
      601 ggtgcacccg cgcacaccag cctgcgccag cagttctcga cgatcgcgcg gcgccaggtc
      661 cggttggtcg tttccgatcg cgcctacttc acttttctgg cgctgttgcc cttcatcctg
      721 ggggcactgt cgctgaccgt tcccgggcac gccgggttcg gcgtcgccga accgaccagc
      781 tcaacccccg acgaagccgc acagatcctg attctgatgt ccatcgccgc cgtcttcatg
      841 ggtaccgcgc tgacgatccg cgacctcatc ggcgagcgcg ccattttccg gcgcgaacag
      901 gcggtggggc tgtcgacggg ggcctacctt accgccaaga tcgccgtgta ctgcgtgttc
      961 gccatcgtcc aggcggcgat cacgacgggc atcgtgctgg tgggcaaggg tgcgcccact
     1021 cagccggccg tgctgctcgg cccgtccacg ttcgcggccg gcgtggagct gttcgtcacc
     1081 gtcgccgcga cgtgtgtggc gtcggcgatc ctggggttgg cgctgtcgtc gatcgcgcgg
     1141 tccaacgagc agatcatgcc gctgctggtg gtgtcactga tgctgcagct ggtgctcgcc
     1201 ggtggcatcg tcccggtgac cggccgcatg gtcctcgacc agatgtcctg gacggtgccc
     1261 tcgcgttggg ggtacgcggc gtcggcgtcc actgtcgacc tgcgggcgct ggtgcccggt
     1321 tcgctcatac ccaaggaccg ccactggttg cacacgcgcg gcgcgtggct gctggacatg
     1381 gggatgctcg ccgcgctgtc gctggtctac gccgtcatcg tgcggtggcg catccggctc
     1441 aagcgctgac cgtcaaccgt ccatactccc ctcgaatcga aggccgtgcg ccaacgcaaa
     1501 agccaacgct tggccgacgt ccacgcaggc gcccgccagc gaggcggtcc gccacagcga
     1561 cgggtccacc gtcgcgccgc gcaaatcggc gtcgtccagc cgggtgccgg tggtccgcgc
     1621 gccgctgaga tcggcgcccc gcagcacggc cttgcgcagg tcggtttcca ccaggctggt
     1681 ctcccgcagc cgacagccgc tcaaatcgac gttccgcagg tcgttgccgc cgagcaccgc
     1741 gagcgtgaaa tccacctcgt cgaacgtgat cggtcgcagc cggcactgca cgaaaaccga
     1801 ccccagcatg ctgcactgcg caaaggtggc gtgccacagc agggatcgct cgaaggtgca
     1861 gttacggaac gccgacccgc ggtgttggga ttcggccagg ttgacgccgc tgaaattgca
     1921 ttcggtgaac actgcccgct cggtgcgcag ccggctgagg tcttcgtcgc gaaaatccct
     1981 gccttcgaaa tggcagtcac tccaatccga ccccggcggc aaagcggtca gctcgcggcc
     2041 aaggcttcca gtgcgtattc gctgaccgcg atgagcgcgt cggtcgccga cctgcgatcc
     2101 ctggcgtcga ccgtgatcac cgggatgtgg gccgccagcg tcagcgcctc gcgcacctcg
     2161 tgcacggggt agcgcggtgc gccgtcgaac tcgttgaccg cgaccaggaa cggcaggttg
     2221 cggtgctcga agaagtcgac cgcggcgaag ctgtcctgca ggcggcggca gtcaaccagg
     2281 acgatcgcgc cgatggcgcc gcgcaccagg tcatcccaca tgaaccagaa ccgccgctgc
     2341 cccggggtgc cgaacaggta gaggaccagg tcctcgtcca gggtgatccg gccgaagtcc
     2401 atcgcgaccg tggtggtccg cttgtccggg gtggtctcga gcatgtcgac gccggccgag
     2461 gcgtccgtga gcatcgcctc ggtgcgcaac ggcatgattt ccgagacagc cccgacgaat
     2521 gtggtcttgc cgacaccgaa cccgcccgcc acgacgatct tcgtcgaagc gtggcccctg
     2581 tcagagtgct ttaaggccac gcagggtcct tcctatgagt tcgtggcgct catcacgggt
     2641 ggaacgctcg gtcaaggttc tatgcacccg aaggtagccg gacaggacca gatcgccgac
     2701 caggacgcgc acgacaccga gcggtaaatc cagccgggcc gagatttccg cgaccgaggg
     2761 gctgtccacg cacagttgaa tgattctgcc tcgtacatcg tcgggcggcc accggtgcgc
     2821 caggcccgct tccaacgtct gtatcggggc ttccaagggc aggtcgacgt cggtgtccgt
     2881 gcgcccggcc gtcagggtat acgggcggac cagattcgct tcaggtgagg gcggccaggt
     2941 gtcgggtgtg tccatcgcgg ctcacgagtg tgcgctgctc ggcgcagacc ggcgggagga
     3001 ctccaccatg ctgcccaccc gttcgaccag gatggccatc tcgtagccga tctgaccgat
     3061 gtcgcaggac gtcgcggcca gcgtggccag gtgtgagcca tcgccgacgc gcatcaacag
     3121 caggtatccg ttctgcatct cgacaaccga ctgcagcacc tgcccgccct cgaacagctg
     3181 cgcggcgccg ctggccaggc tggccagccc ggaggccacc gcggccaact gatcggctcg
     3241 ctcgcgcgga agatgctcgc tggcggccac cggcagcccg tcgacggaga ccaccaaggc
     3301 gtgcgcgaca ccggggacct cgcgggcgaa cttcgtcacc agccagtcca gcgagttgcc
     3361 agctgatgtc attgctgatc ggatccttcg ctcgcgtcac gggcgtgcga ccgggcggag
     3421 cgcacaccgc cgaaatggct gctgaaggac gcgcgaaccg cctcggggtc ccgcgtcgcg
     3481 gacgaatgct gcggctgccg cccggaatga gcgccgttgg aggtgacgag caatccttgg
     3541 ctttggtcct gctggtcctg cgggtcctgt ccggtgaggc tcatcccgtt ggccgcaccg
     3601 ggtaccagcc gggcgccggg tttgcgaacc ggcagcccgt gctcggcggt gtgcgattcc
     3661 acgggcttgt cctcggccgc ggcggccaac gaccagccac ggtcccacac cgactgccaa
     3721 tccaggtcgg ggctgttcac caggtcgtgc ggatcgccca gcatctccga gagcatccgc
     3781 cggtagatga cgtcatcatc ggccggggct gtcgaagccg tcgcggccgg cgggacgacc
     3841 gggatcgagg gctttgagat gtttgccgcg gcggcgggac tcgcgggcct gacaggcgga
     3901 gcttcggcgc ggtcggcggc gctctgcgaa cgcgcggcca agaacgccga ggtgtccgac
     3961 gccgcagcgg ttcggggcgc cgccggcgat tgtttggttt gttgccttgt ctcccaccaa
     4021 ggcgtggcga gctcgcgccg gcgcggggag ggctgctgct cggcgggcgc ggcgggaacg
     4081 tcggtgatgc cgctggaacc cgggttgcgg cgcggcaaca gcgtcaccga aggtcccggc
     4141 tccgtaaagc cttggtggtt ggggtggtct gagtggtttg gggggtttgg gtggtgaggg
     4201 cggtttgctg tctgcggcgc ggcgacgtcg gcgacactgg gcggcgggtc cgcccctgcc
     4261 gtgcgcggct ccggtgcctg agccaccgac acgccctcga gcgccgtcgg cggtaggtag
     4321 atttcggcgg tcgtcccaga tccggcgtcg ttggccgccg ggccgcgcag ccccacccgg
     4381 atgtcgtgcc gggcggcgat ccggcccacc acgaaaagac ccatgtggcg ggcattgtca
     4441 ggagtggggt ccggggtgac ctcgccgccg gcccgtagcc gcatgttggc gatgcgccga
     4501 tccgtgtcgt tcatgcccag gccggagtcg gagatccgca gcaccactcc cccgtcgccg
     4561 ccgcgtgccg cggagacctg cacggatgtc gtcggcggcg aataccgcag ggcgttgtcg
     4621 atcagttcgg cgaacagatg gatgacgccg cccgccaccg agccgatcag cgcgcagtcg
     4681 ggcagcccgg tgatctcgac ccggcggtag tcctcgacct ccgacaccgc ggcattgatc
     4741 accgtggcga gcggcaccgg ctcgcgctgg tcacgcgcga gctgtgcgcc cgccagtacc
     4801 aacaggttgg cgctgttgcg gcgcagccgg gccgccaggt gatcgagccg gaacaggttg
     4861 tcgaggcggt cgggatcctc ctcgtcgcgc tccagccggt cgatcagtgc cagctgctgg
     4921 tcgaccaacg aacggctgcg ccgcgacatg gtctcgaaca tgtcgttgac cagcaatcgc
     4981 aatcgtgcct cgtcgccggc cagcagcagg gcctgcgtgt gcagttcgtc gaccgcgtgg
     5041 gcgacctgcc cgatctcctc ggtggtgtac accggcaacg gctgcaggat cggctcggcg
     5101 ccaccggctc tgacccgggc cacctcctcc tccaggtcgg tgtgggcgac ccggagcgcg
     5161 ccgtcgcgca ggacgcgcag cggccgcacc agcgtccgcg ccatcaccaa aacgatggcc
     5221 agtgcgatca cgacggccga cagcaccagg acggtgtcgc ggatcgcggc attacggcga
     5281 tcggcggcct cggcatgcac cgacttcgtc accgacgcgg tggcgtcgtc ggtgacctgt
     5341 tcggcgatcg tgtcggtggt ctggatcgag cgcagcagct cggggttgtc gacgagcacg
     5401 ctggccggat cggacatgat cgccatccgg ttcaccatct gctgctgcag cgtcttggct
     5461 tcgggcgacg aggcgccgag cacttcgctc atcccgaaca acgtcgacgg ttcggtgccg
     5521 gccaaggtga tcatcgaggt ccgcagttgt ggctcgggaa ggtcagcgcc gcgggtcacc
     5581 aggatttcct gcatcgtcat ctgcccgcgg gcgccgacgg ctcggctcaa accctgcgcc
     5641 tcggcgcgga tctgctcgtc gccgacgcgc accgaggcgt tgatgacgtc ctcggcggtc
     5701 aacaggatcg gtgcgtaggt ggtcacccgg tcccgcaaac cgatgctgtt gtcggccacc
     5761 ttgtccagca gcccctggcc gccgttgatc agggtgctta ctcccgatcg gacgtcgggg
     5821 gtgacgtcgg tgtcggccag ccgcgtctgc agttcgtact tgcgcgcgtc gtagttcttt
     5881 ttcgccccgt caacgtcgcg tccggtggag ctcgccagca gcgcgacatc caacgccgac
     5941 atgtacttgg tgatcgccgg cagcacctcg gcgcggtcgg ccgccagatt gaggttgcgc
     6001 gagccggcca tcgcatcctt gacccgcagc gcgccgaacg ccgtcgccaa aaccagcggt
     6061 accagcacaa tcgccaaaac tttggagcgg accggccagt tgctcggcga ccacgccggc
     6121 ggacgcttgg ccggcgtcgc ctcggccgcc gggatgggtg tgggttcggg cagagccgcc
     6181 tcgacggaat gggccgggcg ggtgaacatc gtcacgttgt cacagccgcg cgactggccg
     6241 ctgcgccgat tatcggcgct cgcaaaaaca agaggctcat aaaacttcct ggtttttccg
     6301 cccttatgcc acccgtcacg cgcaaaaagg cgcgagcgaa tactcctggc aatccgacga
     6361 gtatgacagc ccgcggccga ggtctccaca attgttactg attaggcaga gccctccaag
     6421 gttgggccat gccccggtgc gcaagtttgg caaacaaacg gctttgtcat gatcggcaga
     6481 tgttccggct gttgttctat tccccgcgta tcgcccccaa cacgggcaac gccatcagga
     6541 cggtggccgc caccggcgcc gaattgcatc tggtcgagcc gctgggtttc gacttgtccg
     6601 aacccaaact ccggcgggcc ggtctcgatt atcacgacct cgcgtcggtc accgtccatc
     6661 cttcgcttcc ggccgcgtgg gatgcgctgt cgccgcagcg agtgatcgcg ttcaccgcgc
     6721 acgccacgac ctcgtttgcc gacgtcgact accacgccgg cgacgtgttg atgttcgggc
     6781 cggaacccga cgggctggac gcggcgacgc tggccgacgc ccacatcacc gggcaggtgc
     6841 gcattcccat gctggccggc cggcgctcgc tgaacctgtc caacgcggcg gcgatcgccg
     6901 tctacgaggc ctggcggcag cacggctacg ccggcgcaat ctagccctcg cgatcgccac
     6961 ggtctgatgg tggcgacccg cttcgcccgg ctacgccgcc ctcgcgatcg ccacgggcta
     7021 ccaggtgttc cagtgcgtca ggctctcggc gggcagcctc ttggccggcc ggaagtcggt
     7081 gcctttggtg taggcgatcg gaaacaggcc ggcctggctg taccggtcgt cgggaatgcc
     7141 gagtatttcg gcggccttct tctcgccgcc ggcgagaagg tgcagcgtgg tccagcagga
     7201 gccgagtcca cgggagcgca gcgccaggca gaagctccag accgctggga acagcgacgc
     7261 ccagaacgac gcaccgccca atggctgctt gtcttcccga ccgtaaatgc aggggatcat
     7321 caggaccggt gcctcgtgca tgtgctcggc gaggtaggtc gcggaatctt tgaccttgcc
     7381 catccgctca ccgcgggtgt caccgtcggc gtattcgggc gacggcgagc tgaggtaggt
     7441 gcgggcgttg gccaggtaga tgtcgccgat cgccttcttc ttttcggcgt cctcgacgaa
     7501 cacccattgc cagccctgcg agttggaccc agtgggcgcc tgcagcgcca gctcgaggca
     7561 ttccatcagc acgtcgcggc ctacgggctt gtcgaaatcg aggcgtttgc ggaccgagcg
     7621 ggtggtggtg aggacttcgt cgaacgacaa gttaagggtc atgtggggag actactcagc
     7681 ccccgcgagg gagcggtatc agcggaagtc gcgcgacttc gatccgacga caaggctgat
     7741 tcggcccaac cgttcggcca cgacggtgac cgccccgctg gcgttttgga cctgcccccg
     7801 gatgagcagc gccggtgccg tattcgccag cttgcgatgc cgcgcccaca ccccctgcgt
     7861 gcaaagcacg ttgaccatcc cggtctcgtc ctcgaggttg atgaacgtca ccccctgggc
     7921 cgtcgcgggt cgctgccgat gggtcaccgc accggcgatc agcacccggt cgccgtcggg
     7981 cacgctcagc agcgcctcgg cgggcaccac gcccatcgcg tccaggtcgg cccgcagaaa
     8041 ctgcgtggga tagctgtccg gggagacgcc ggtggcccac acgtcggcgg cggccaactc
     8101 cagctcactc atccccggca acggcgggat gtgcgacgac gaccccacac cgggtaaccg
     8161 gtccggccgc tgcgtagcgg ccgccccggc cgcccacaac gcctcccgcc gggacatgcc
     8221 gaaacagccc agcgctcccg ccgtggccag cgcctcggtc tgcggcacgg agagctgcaa
     8281 ccgggacgtc agatccaaca gagaagcgaa cgggccgttg gtttttcgct cttcgaccag
     8341 ctgctcggcg aggtcgtcgc cgatgtggcg cacggcgccc agaccgaggc gaacggtgtt
     8401 ccccgcatct tccagggtgg catgggccag gctggcgttg acgtccgggc cgtgcaccgt
     8461 cacaccgtgc ctgcgcgcat cggccaccag cgactgcggt gaatagaaac ccatcggctg
     8521 cgcgcgcagc agcgccgcgc agaacgccgc cgggtgatgc agcttgaacc acgaagagta
     8581 gaacaccagc gacgcgaagc tcagggcgtg gctttccgga aagccgaaat tggcgaacgc
     8641 ctccagcttt tcgtaggtcc ggtcgatcac ctcgtcggtg gcgccgtgca gcgcacgcat
     8701 gccgtcgtag aaccgtccgc gcaaccgtcg catgcgttcg gtcgaccgct tcgatcccat
     8761 ggcacgccgg agctggtcgg cctcggcggc ggaaaatccg gcacagtcca ccgcgagctg
     8821 catcagctgc tcctgaaaaa gcggcactcc caacgtcttt cgcaatgccg actccatgga
     8881 cgggtggtcg tagacgaccg ggtcgatgcc gttgcgccgc cggatgtacg ggtgtaccga
     8941 gccgccctgg atgggcccgg gtcggatcag cgcgacctcc accaccaggt cgtagaacac
     9001 ccgcggccgc agccgcggca gggtggccat ctgcgcccgc gactccacct ggaacacgcc
     9061 gacggaatcg gcgcggcaca gcatttcgta caccgccggc tcggagaggt ctagcctggc
     9121 cagatccacc tcgatgcctt tgtgctcggc caccaggtcg atcacatagt gcagcgccga
     9181 gagcatgccc agcccgagca ggtcgaactt caccagaccg attgccgcgc agtcgtcttt
     9241 gtcccattgc aggacgctgc ggttctccat gcgcgcccac tccaccggac atacgtcggc
     9301 gatcgggcgg tcacagatca ccatgccgcc ggaatggatg cccatatgcc ttggcagatt
     9361 acggatctgg gtggccagat cgatcacctg cagcggaatg ccgtccaggt ccggcgagtc
     9421 ggccagccca ttccaatgac tgatctgctt gctccacgcg tcctgctgcc cctgcgagaa
     9481 gcccagggcg cgggccatgt cgcgcaccgc gatccggccc cgataggtga tcacgttggc
     9541 gacctgggcg gcgtagtcac ggccgtactt gtcgtagacg tactggatga ccttctcgcg
     9601 ctggtccgac tcgatgtcca tgtcgatgtc gggcggtccg tcgcgggccg gcgacaggaa
     9661 gcgttcgaac aacagctcgt tggccaccgg atcgactgcg gtgacgccca gggcatagca
     9721 gaccgcggaa ttggccgccg atcccctgcc ctgacacagg atgttgttct cccggcagaa
     9781 ccgggcgata tcgtgcacca ccaggaagta gcccggaaat gtcagctgcg caatgacttt
     9841 cagctcacgc tcgatctgag agtatgcgcg ggacgcgccg tcggggagtc cgtagcggtc
     9901 gcgggccccc gccatcgtca actgccgcag ccagctgtcc tcggtgtgcc cgtcgggcac
     9961 gtcgaacggc ggcagccgcg gtgcgatgag cgccagcccg aacgcgcact gctcgccgag
    10021 ttcggcggcg gcggccacca cgtcagggcc cccgaggggc acatgcgcga acagccgggc
    10081 catctcctcg ccggaccgta ggtgcgagcc acccagcggt gccagccacc ccgcggccga
    10141 atccagggac tgccgggccc ggatggaacc catcgccatg gccagccggc ttcgcgacgg
    10201 atgggcgaag tgtgctccgg tggtggcgac gacgccgaca ccgaagcgcg gcgccaggcc
    10261 ggccaatgtc gcgttgtgtt cgtcgtccag cggatgacca tgatgggtca gctcgatact
    10321 cacccgcccg gccccgaacc ggtccaccag atcggccagc gcccgcgccg ccgcgtccgg
    10381 accgccgtcg gaaagcgcgt ggcggacggt gcctttgcgg catccggtca ggatgtgcca
    10441 gtgtccaccg gcggcctcgg tcagcgcgtc gatgtgatag cgcggcttgc ctttctcccc
    10501 gccggccaga tgtgccgcgg ccagctgccg cgacagccgt cgatagcctt ccgggccgcg
    10561 ggccagcacc agcagatgcg ggccgggcgg atcgggctgc tcggtgcggg caaccgaccc
    10621 cagcgacagc tcggcaccga acacggtacg cacatcgagt tcggtggcgg cttcggcgaa
    10681 ccgcaccgcc ccgtacagcc cgttgtggtc ggtcagcgcc agggcacgaa gacccagccg
    10741 ggcggcctcc tcgaccagtt cctcgggcgt gctggccccg tcaaggaagc tgtacgccga
    10801 gtgcgcatgc agctcggcgt atggaacgga atgagccacg gaggaacggg ccgcacggcc
    10861 tgagctcggc ggctggtatg tcccgcgttt gtgcgatagg ggaacgtctc cgccggggtc
    10921 gaaagccggc tcggcggccg gtgcgccggc gtggcggggc ttgccgtcga gcacccgctc
    10981 catttccgcc cagctcggcg gcccattaaa ccagcccacg tggacattct atcgaatgtt
    11041 tgttcgattc gatccagtgc tgttaaacgc ttcttcaact tctaaacgcg ttcttaacgc
    11101 taggggccta tcgttctacg agacctgcac agatactggt acaagcgcaa gactagggtg
    11161 gggacaagca tggcagagct cacgacattg gatacgggtc ctctcgggac ccaggacccc
    11221 gatcgccagg tcagcctggc gatcggcgcg gtcgccgttg tcgacggcgc cgcccctgac
    11281 cacgatctac tcaaaagagt tctggaagaa cggattcagt cgataccgcg atgcacgcag
    11341 gtattgcgta cccacccgtt caacggcgcg cagcagtggg tcgacgaccc gaggttcgac
    11401 ttcgaccacc atgtgcgccg agtgacgatt ccgcgccccg gcgacgaggc cgaactgtcc
    11461 cgggccatcg cgcaggccct ggagcgtccc ctggacccgg accgcccgct gtgggagtgc
    11521 tgggtcatcg agggcctcaa gggcaaccgg tgggcgatct tgatgaagat ccaccactgc
    11581 ctgtcagaag gcaattcggc ggcccacctg ctcgcccggc tctgcgacga agccgacacc
    11641 gacatattcg tcaaccacgt tggctccaag catgtttcac catcgcaaac cccgaagcga
    11701 agcttgttcg acgccctgtg gcgcgcgtcg accatcgccg gcaacgtcac caacaccctg
    11761 gccgggagca tctggtctgc ggtgcggtcg ccgtcgacga tcccggtcaa caccaggcgg
    11821 cgctaccgca cggtgcgcgt tcccctcacc gccgtcgacg acgtgtgccg caagttcggg
    11881 gtcaccacca atgacgtcgc gctcgcggcc atcaccgagg gcttccggac ggtgctgctg
    11941 caccgcggtg aacaaccgcg cgcggactcg ctgcgcaccc tggacaagac cgacaatcgg
    12001 atctcggcga tgctgccgta cctgcccgtc gaacacgacg atccggttca gcggctgcag
    12061 acggtgcaca gcaggttgaa ccggcccaag cagacgagcc agcgcccgac tccggccatc
    12121 gtgcggttgg cgaacagcta cccgccattc gtgttgtccg ccaagctcgc caaattgctg
    12181 ctgagccgac tgccgcaacc ggggatcgtg acgctggcga ccaacgcgcc cggcccacgc
    12241 caccggctgg ggctgatggg gagaacggtg gagcgtttgc tgcccatccc gccgacggct
    12301 tcgcagctgt ccagcggggt tgccgtgctc agttacggcg atgagctggt tttcgggatc
    12361 accgccgact acgacaacgc ggccgaactc gagcagctcg cggtcggcat cgaacgcggc
    12421 atggcacgtc tggtggcgct cagccaggac tccgttctgc tattcaccaa agagagccgc
    12481 aagcgcccgg cgcgcgccct tcccaccgcc gcccagcgcg ggcggccgtc cgcgcccgga
    12541 cgaccctgac ccaaccggac ctttctccgg cggcgcccag caccgtgaga gggttggggc
    12601 gtgcccaact tggagccggt catcgacccg cgccgtcatg acgccgtgct gttcgacct
//
LOCUS       DASSGQ010000110         6881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_676278,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000110 DASSGQ010000000
VERSION     DASSGQ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676278"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFI55_05100"
     CDS             <1..357
                     /locus_tag="VFI55_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HET7073444.1"
                     /translation="EIPDVITQALSELRLAGVDGPYSVLLAADVYTKVSETTEHGYPI
                     LEHINRLVNGDIIWAPAIDGAFVLTTRGGDFDLQLGTDVSIGYTSHDADTVQLYLQET
                     LTFLCYTAEASVALGP"
     gene            complement(367..2079)
                     /locus_tag="VFI55_05105"
     CDS             complement(367..2079)
                     /locus_tag="VFI55_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HET7073445.1"
                     /translation="MGRLVSVNVGMPKDVRWRDRTVYTGIWKAPVEGPVMVRRLNIDG
                     DGQGDLAGHGGEQRAVMVYQTESYAFWKTYLGRDDLLPGHFGENFTVTGLADDQVCIG
                     DRYRIGDAEFEVTQPRVTCFRVGLRLNKPEMPNLLVSQHRPGFYFRVIGEGQVRAGDD
                     IVRTARGRHELSVADVDALLYLPNRDIDQLRKIVDVPALSPGWQQSFRDMLKAHETSG
                     AQSAPPVGVEPGWNGFRPLRVSAVCRESPQVLSIRLESDDPGRLPPPLPGQYLTVRIP
                     GAGDPAPLRSYSLSGDPAAPDYRISVKREDHGLVSHWLHDHIQPGSVLEAAAPRGEFY
                     LTEGANPVALISAGIGITPVLAMLHTLAADRSTREIWWVHTTRNRETSAFSAELATLI
                     QSLPHARQLVVYTQTQGRLDSSRIAGLRLPAEAVAYLCGPTQFMTDMRDALTAAGLDP
                     ARIHSELFGALPAINPGIVDGAPRKPPHPPAGAPGTGPSITFARSGLTVNWSPDYGTI
                     LDLAEACDVPTRFACRSGVCHVCVTGVVAGTTTYVRAPLEPPEQGSVLICSAAPGSDV
                     VLDL"
     gene            complement(2103..2819)
                     /locus_tag="VFI55_05110"
     CDS             complement(2103..2819)
                     /locus_tag="VFI55_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7073446.1"
                     /translation="MGDTLRDKTVVVVGRGSGIARAVALRAQSEGARVIVAGRDPAKL
                     AGAYSGGDVTAEVVDITDDGSIAGLADRVGAVDHVVSTASARARGKLGDLDRHKLQLS
                     FDTKVIGPTMLAKYFAPQVNPGGSFVLFSGVHAFKLNVGYLGVGITNGAVDFLTRWLA
                     VELAPIRVNAISPGVIDTGAWDSLGDEGKRDYFKQIAENNPARRIGTPDDIADAVLFA
                     MTNTFLTGVTLKVDGGEPLI"
     gene            3122..3994
                     /locus_tag="VFI55_05115"
     CDS             3122..3994
                     /locus_tag="VFI55_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7073447.1"
                     /translation="MPAVHHRYATVEGHRLFYREAGEPDSPALVLLHGFPTSSYMFRN
                     LVPALADRYHVIAPDHLGFGLSDAPPVEEFDYTFDALTDLTAGLLRSLGVDRFSIYVQ
                     DYGAPIGWRLALREPSAITAIISQSGNGYDAGFVESFWKVVQAYQNEQTADTEAPVRQ
                     FLTLGATRWQYLTGVPDETLVDPESWYHDFALISRPGNDLVQLKLLRDYATNAPLYPR
                     VHEYFRASRVPLLAVWGRGDEIFGPAGAEAFADDLPDAEIHLLDGGHFLLESALDEVA
                     ELIRGFLAKVPRPD"
     gene            4095..4790
                     /locus_tag="VFI55_05120"
     CDS             4095..4790
                     /locus_tag="VFI55_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7073448.1"
                     /translation="MVHPDVQDEQRLTAKGRATRDRIVRAAAELIVTEGLSASNMENV
                     RRAAAVSGSQLAHYFADKRALIRAVIRRQIGVVLDFHQQPKLRGLASFDDFERWIDLN
                     MRYLRRIGYFGTPTYHALAAQLAKSDDGIREALAAGYWQWIELLAQAIQRMKDAGVLV
                     DGADPRRLALAMVSAHQGGGTLAFIYRAEWPHADAVRFAVNYLRMFAADPADRIPRPP
                     RRRRGPRPTRKAS"
     gene            4815..5483
                     /locus_tag="VFI55_05125"
     CDS             4815..5483
                     /locus_tag="VFI55_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7073449.1"
                     /translation="MSDHTTSRFTRKGLATRARIVDVAARLMFERGVANTSIDQVRRI
                     AEVGGSQISHYFRDKRDLTRHVVAARRNEVRAFHTQPQFDSLDSLDALQAWADACVSD
                     IDAVYRVGGCVYGSLAGELIEGDDEIHDDLSTGYDEWIALFEAGLTAMHRRGDLRADA
                     DPRHLAVSLVTAHQGGAMITYATGDPEPLRVAVTAAVDYVRSFAPPAPRESRSTRSRR
                     TAKS"
     gene            5643..6701
                     /locus_tag="VFI55_05130"
     CDS             5643..6701
                     /locus_tag="VFI55_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HET7073450.1"
                     /translation="MAATSSPPLPTAPAVVREAPGGETMRAIILNGFGGLDSLVYTDI
                     PKPLPKDGEVVIEVKGFGINHAELHMRRGEWAEAAEVSGIECVGIVDSCPGGEFPVGA
                     KVAALMGGLGRTVNGSYAEYTRVRVANAAPIESDLPWSDLAALPETYAVAWTCLFRNL
                     DLKAGQTLVLRGATSSLGQAALKMAVAAGARVIATTRSTKRFSMLEELGASRVELERR
                     DLAAHLAESKTEAKRVDSVLDLVGNSTILDSLDMLCRGGTACLAGWLGGLDPIGDFNP
                     LLRMASGVNWNFFGSFVFGNPGFPLSDVPLQDIARQVAEGKLDAKPSRVFSFNQIHEA
                     HRVMEAGEATGKMVVVMS"
BASE COUNT         1162 a         2312 c         2245 g         1162 t
ORIGIN      
        1 gaaatccccg acgtcatcac ccaggcgctg tcggaactgc ggctggccgg cgtggacggc
       61 ccctattcgg tgctgctggc cgccgacgtc tacaccaagg tcagcgagac caccgagcac
      121 ggatatccga tcctcgagca catcaaccgg ctggttaacg gagacatcat ctgggctccg
      181 gccatcgacg gcgcttttgt gctgaccacc cgcgggggcg acttcgacct gcaactgggc
      241 accgacgtct ctatcggata caccagccac gacgcggaca cagtccagct gtatctgcag
      301 gagacgctga cgttcctgtg ctacaccgcc gaggcgtcgg tcgcgctcgg cccgtagctc
      361 gagtcgctac aggtccaata cgacgtcgct gccgggcgcg gccgagcaga tgagcaccga
      421 tccctgctcg gggggctcca gcggggcccg gacgtaggtt gtcgtccccg ccacgactcc
      481 ggtgacacag acgtggcaga ccccgcttcg gcacgcgaag cgggttggca cgtcgcacgc
      541 ctcggccaga tcgaggatgg tgccgtaatc cggtgaccag ttgaccgtga gcccgctgcg
      601 ggcgaacgtg attgacggcc cggttccggg cgctccggcg ggcgggtgcg gcggtttgcg
      661 cggagcgccg tcgacgatgc ccgggttgat cgcggggagc gcgccgaaca attcgctgtg
      721 gatccgcgcg gggtcgaggc ccgcggcggt gagggcatca cgcatatccg tcatgaattg
      781 cgttgggccg caaaggtatg cgacggcttc agcgggcagc ctcagccccg cgatgcgcga
      841 ggagtccaga cgcccctgcg tctgcgtata gacaaccagc tgccgggcat gcggcaacga
      901 ttgaatcagc gtcgcgagtt cggctgaaaa cgcggaggtt tcgcgattgc gggtggtgtg
      961 cacccaccag atctcgcggg tactgcgatc agccgccagc gtatgcaaca tggccaagac
     1021 cggtgtgata ccgattcccg ccgagatcag ggcgaccggg ttggcacctt cggtgaggta
     1081 gaactcgccg cgcggggcgg cggcctcgag gaccgatccg ggctggatgt ggtcgtgcag
     1141 ccagtggctc accagaccgt gatcctcgcg cttgacgctg atgcggtagt cgggcgcggc
     1201 ggggtcaccc gacagggagt aactgcgcag cggcgccggg tcgccggcac cgggaatcct
     1261 caccgtcagg tattggccgg gaagcggcgg cggcagccgt ccagggtcgt cggactccag
     1321 gcggatggac agcacctgcg ggctttcgcg gcacactgcg ctgacccgta acggtcgaaa
     1381 cccgttccac cccggctcga ccccaaccgg gggtgcgctc tgggccccgg aagtctcgtg
     1441 cgctttgagc atgtcgcgga acgactgctg ccagccgggg ctgagcgcgg gaacgtcgac
     1501 gatcttgcgg agttggtcga tgtcacggtt cggcaggtac aggagcgcgt cgacgtcggc
     1561 gacgctgagt tcgtggcggc cacgggcggt gcgaacgatg tcgtcaccgg cacggacctg
     1621 cccttccccg atgacccgga agtagaagcc cggacggtgt tgggagacaa gcaggttggg
     1681 catctccggc ttgttcagcc gcaggccaac ccgaaagcag gtgacgcgcg gctgggtgac
     1741 ttcgaattcg gcgtcgccga tgcggtaacg gtcaccgatg cacacctggt cgtccgccag
     1801 cccggtgacg gtgaagtttt cgccgaagtg gccgggcagg aggtcgtcgc gacccagata
     1861 ggtcttccag aaggcgtacg actcggtctg gtacaccatg acggcgcgtt gttcaccgcc
     1921 gtggccggcg agatcgccct gaccgtcgcc gtcgatgttg agccggcgca ccatgaccgg
     1981 tccctcgacc ggcgctttcc atatcccggt gtagacggtc ctgtcccgcc atcggacgtc
     2041 cttgggcatg cccacgttga ccgaaacgag cctacccacc gcgtctcctt gactacccgg
     2101 agctagatca gcggttcgcc gccgtccacc ttcaaggtca cacctgtcag aaaagtattg
     2161 gtcatcgcga acagcacggc atcggcgatg tcgtcaggag ttccgattcg ccgtgccggg
     2221 ttgttctcgg cgatctgctt gaaatagtcc cgcttgccct cgtcgccgag cgagtcccag
     2281 gctccggtat cgatgactcc gggagagatc gcgttgaccc tgatcggggc gagttcgacg
     2341 gccagccagc gggtgaggaa gtcgacggca ccattggtga tacccacgcc gagatagccg
     2401 acgttgagct tgaatgcatg aacccccgag aacagcacga acgagccgcc cggattaact
     2461 tgcggcgcaa agtatttggc cagcatggtg ggcccgatga ccttggtgtc gaacgagagc
     2521 tgcaacttgt ggcggtcgag gtcgcccagc tttccgcggg ctcgagccga ggccgtcgac
     2581 accacgtggt caaccgcgcc cacccggtcc gcgaggccgg cgatcgatcc gtcgtcggtg
     2641 atgtcgacca cctcggcggt gacgtcgccg ccgctgtagg cgccggcaag tttggccggg
     2701 tcccgtccgg ccacgatgac cctcgcgcct tcggattgcg cccgcagggc gacggcacgc
     2761 gcgatcccgc tgccacgtcc gaccaccacg actgtcttgt cccgcaaggt gtcacccatc
     2821 gtgcgctcgc tcctcgggtg cggttggccg acggctgcct acgcgcccgc cgtcaccgca
     2881 aagaccgggc gcaaattggg atggttggcc cagaatatgc cagcccgatc gccagggcaa
     2941 caccggaccg gcgggacccc gatcggccgt gagtgtgctg cccgagactg ttgcgcgccg
     3001 cccactttgg tgcatactgg gtcatacaac ccagaaatgc agcagtgtga gtttgtgaac
     3061 ttgcggcgaa cgcccgcagg gcgctcgatc gtgaatcgca cgtgggctca ggaggtatct
     3121 catgccggcc gttcatcacc gctacgccac cgtcgagggg catcgactgt tctaccgtga
     3181 agccggcgaa cccgattcgc ccgcgctggt gctgttgcac gggtttccga ccagttccta
     3241 catgttccgc aacctggttc cggccctcgc ggaccgctac cacgtgatcg caccggacca
     3301 cttgggcttc gggctgtccg acgcaccgcc cgtcgaggag ttcgactaca cattcgatgc
     3361 gctgaccgac ctcaccgcgg gcctgctgcg ctcccttggc gttgatcggt tctcgatata
     3421 cgtgcaggac tatggcgccc ccatcggctg gcggctggcc cttcgcgagc cgtccgcaat
     3481 cacggcgatc atcagccaaa gcggcaacgg ctacgacgcc ggcttcgtgg agagcttttg
     3541 gaaggtcgtg caggcctacc aaaacgaaca gacggcggac accgaggccc cggtccggca
     3601 gttcctcacc ttgggcgcca cccggtggca gtacctcacc ggcgtacccg acgagacgct
     3661 ggtcgacccc gaatcctggt atcacgactt cgcgctgatc tcccggcccg gcaacgacct
     3721 cgtgcagctg aaactgttgc gggactatgc caccaatgcg ccgctctacc cccgcgtgca
     3781 cgaatacttc cgcgccagcc gggtgccact gctggcggtg tggggccgcg gcgacgaaat
     3841 cttcggcccg gccggcgccg aggcgttcgc cgacgacctg cccgatgccg agatccatct
     3901 gcttgacggc gggcacttcc tgctggagtc cgcactggac gaagtcgccg agctgattcg
     3961 cggctttctc gccaaggttc cccggcccga ctgacgtccg gtgggcggcg ggttccagcc
     4021 ggtcaatctt tccagtcttc gccagtgggc tggatatccc ataattccgg gggttcgcgg
     4081 gttagcctga cgcgatggtc catcccgatg ttcaggacga acaacggttg accgccaaag
     4141 ggcgcgccac ccgcgatcga atcgtgcggg cggccgccga gttgatcgtc accgaaggcc
     4201 tctccgcgtc gaacatggag aacgtgcgcc gagcggcagc ggtcagcggc tcgcagctcg
     4261 cccactattt cgccgacaaa cgcgcgctga ttcgcgcggt gatcaggcgc cagatcggcg
     4321 tggttctgga ctttcaccaa cagcccaagc ttcgcggcct ggcgtcgttc gacgacttcg
     4381 agcgctggat cgacctgaac atgcgctacc tcaggcggat cgggtatttc ggcacgccca
     4441 cctaccatgc cctcgccgcg cagctggcga agtccgacga cggtatacgc gaggcgttgg
     4501 cggccggcta ctggcagtgg atcgagttgc tggcgcaggc catccagcga atgaaggatg
     4561 ccggcgttct ggtcgacggc gccgacccgc gacggctggc attggccatg gtcagcgctc
     4621 accagggcgg cggcacgttg gccttcatct accgcgccga atggccgcac gctgatgcgg
     4681 tccgcttcgc ggtcaactac ctgcgcatgt tcgccgctga tcccgccgat cggattcccc
     4741 gcccaccgcg ccgccggcgc ggcccgcgcc cgacgcggaa agccagctga cccatctgcg
     4801 ccggaggtgg caacgtgtcc gaccacacca cgtcgcggtt cacccgcaag ggtctggcga
     4861 ctcgtgcgcg catcgtcgac gtcgccgccc gtctcatgtt cgagcgcggc gtcgcgaaca
     4921 ccagcatcga ccaggtgcgg cgcatcgccg aagtgggtgg gtcgcagatt tcccactatt
     4981 tccgcgacaa gcgcgacctg acccgtcatg tcgtcgccgc acgccgcaac gaggtgcggg
     5041 ccttccacac gcaaccgcag ttcgactccc tcgacagcct cgatgccctg caggcgtggg
     5101 ccgacgcctg cgtgtccgac atcgacgccg tgtaccgcgt cggtggctgc gtctacggtt
     5161 ccctggccgg tgagctgatc gagggcgacg acgaaatcca cgacgacctg tccaccggct
     5221 atgacgagtg gattgcgctc tttgaggccg gcctcaccgc gatgcaccgc cgtggcgacc
     5281 tgcgcgccga cgccgacccc cgacacctcg cggtgtcgct ggtcaccgca caccagggcg
     5341 gcgcgatgat cacatacgcc accggcgatc ccgaaccgct gcgggtcgcc gtcacggccg
     5401 cggtcgacta cgtgcgctcc ttcgccccac ccgcacctcg cgaaagccga tccacccgat
     5461 ctcggcgcac ggccaagtcc tgacctggtg ccgcgccctg gacatgtttg ggtccactgg
     5521 cccataataa tgggtcagct ggcccattaa gtttggcgcg gacggacgcg gacgctgttg
     5581 ccgtaggttc gcttcgcgcg tcggtatccg aaggcctact aacgatttgg gaagaggcag
     5641 aaatggccgc caccagttca cccccactgc ccaccgcacc ggcggtggta cgcgaagcac
     5701 ccggcgggga aaccatgcgc gcgatcatcc tgaacgggtt cggcgggctc gacagcctgg
     5761 tctacaccga catcccgaag ccgctgccca aggacggcga agtggtgatc gaagtgaagg
     5821 gattcgggat caaccacgcc gagctgcaca tgcgccgcgg tgagtgggcg gaggccgcgg
     5881 aagtcagcgg catcgaatgt gtcggcatcg tcgactcgtg cccgggaggc gagtttccgg
     5941 tcggcgccaa ggtggccgcc ctgatgggcg gtttggggcg gaccgtcaac ggcagctacg
     6001 ccgaatacac ccgcgtgcgc gtggcgaacg ccgcccccat cgaatccgac ctgccttggt
     6061 cagatttggc ggcgctgccc gaaacttacg ccgtcgcttg gacttgcctg ttccgcaacc
     6121 tcgacctgaa agccggacag acgttggtgc tgcgcggcgc gacgtcatcg ttggggcagg
     6181 ccgccctcaa gatggcggtc gccgccggag cgcgggtcat cgccaccacg cgcagcacca
     6241 agcgcttttc gatgctcgaa gagctgggtg catcccgcgt cgagttggag cgccgcgacc
     6301 tggccgccca cctcgctgaa tcgaaaaccg aagccaagcg agtcgattcc gtcctggacc
     6361 tggtgggcaa cagcaccatc ctcgactcgc tcgacatgct gtgccgcggc ggtacggcct
     6421 gcctcgcggg atggctgggt ggacttgacc ccatcggtga cttcaatccg ctgctgcgca
     6481 tggccagcgg cgttaattgg aacttcttcg gcagcttcgt gtttgggaat cccggcttcc
     6541 cgctctccga cgtgccgcta caggacatcg cccggcaggt cgccgaaggc aagctggatg
     6601 caaagccgtc ccgcgtcttc tccttcaacc agattcacga agcacaccga gtgatggaag
     6661 ccggagaagc gaccggcaag atggtcgtcg tgatgtcgtg agccgcacag cgttgagcga
     6721 ccgcaaatag gcactctggc aacaggtttc ggagagcgaa gatacgacaa tggttatcgt
     6781 gacggcagac ggccaggggt cgagcccggc cagacccaac gaacgaccgg catgaataca
     6841 caccccaggc ccccggcacc gtctgtactc gtcttcgatg t
//
LOCUS       DASSGQ010000111        22797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_797920,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000111 DASSGQ010000000
VERSION     DASSGQ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 22797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22797
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797920"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFI55_05135"
     CDS             <1..252
                     /locus_tag="VFI55_05135"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875370.1"
                     /note="catalyzes the formation of
                     (R)-4'-phosphopantothenate in coenzyme A biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HET7073451.1"
                     /translation="DIEQWYVSRFLSLRGTAFADPESHFHHYSALDDEKAVVAAREIW
                     RSINRPNLVENILPTRPRATLVLRKDADHSINRLRLRKL"
     gene            complement(338..760)
                     /locus_tag="VFI55_05140"
     CDS             complement(338..760)
                     /locus_tag="VFI55_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073452.1"
                     /translation="MTSVSGASGLATRPLYDSTDSLLRFAMRADATLTGLCGLAAAFV
                     ADPLSSLTGLTSGQEYALGAFFVLYGLAVFSLAALPNLRRVGIGVVAANIVYTLAAIV
                     AAGVVPMTATGVAATLASGVYTAAFAGLQYWGVRRLAA"
     gene            complement(987..2405)
                     /locus_tag="VFI55_05145"
     CDS             complement(987..2405)
                     /locus_tag="VFI55_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073453.1"
                     /translation="MAALSAVVFTLSGWLGLYLLARDPRKPVLVLAAVGLCGFALVVA
                     LDAVRTAGSHTFQAHAALLSGVEIYLVAVPSVAWFAVLVELSRPCDHWRARTGELLFI
                     GGVGALTLLGAALAGSVDGPLRAGHMVMFAVISVSTLGAMFVALRRSARPVPAGRRGM
                     GLIIVATLFFALGNAILIIPLGLLPSWLALASTGFDLLLLGVAVALWDAFDEGQALRA
                     DMLRSFAGCSVVASLFGGQVLIGLALTRDEPAAHTVLTVLLFTTLAIAIMVQVLADPL
                     AGVLDRMAFSKSPTLRADRAALRHTEAALPLRSVDPRRDIHDVDDETFTRLTRRALGH
                     YADLTKLVASPLTALPVIDERLAARGAPDHPVERANELKAVLADGIARLKPRDGGDFG
                     TTEEWRFYNSLYFPYVVGVRAYAQNATAAGLDPTARQAWQWLVTEVPQRSLHNWQNAA
                     ARLIAADLRGRVPVPTDQASAR"
     gene            complement(2452..3240)
                     /locus_tag="VFI55_05150"
     CDS             complement(2452..3240)
                     /locus_tag="VFI55_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET7073454.1"
                     /translation="MEIIPPRLKEPLYRVYEMRLRQGLAASKSQLPRHIAVLCDGNRR
                     WARDAGYDDVSYGYRMGAAKIAEMLRWCQEAGIEMTTVYLLSAENLRRDPDELAALIE
                     IITDVVEEICAPANRWSVRTVGDLALIGQEPARRLRDAVESTPAVAPFHVNVAVGYGG
                     RREIVDAVRALLSKQLANGATAEELIDAVTVEGISENLYTSGQPDPDLVIRTSGEQRL
                     SGFLLWQSAYSEMWFTEAHWPAFRRVDFLRALRDYSHRHRRYGK"
     gene            3402..4136
                     /locus_tag="VFI55_05155"
     CDS             3402..4136
                     /locus_tag="VFI55_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET7073455.1"
                     /translation="MTQTSTASHAEPESPNLTANTAHQLVDGVARVLTKPRFRGWIHV
                     YSAGVAVAAGASLIAVSWAVGSTRAGLATFAYTAATILMFTVSATYHRVHWKSATKQK
                     WMKRLDHSMIFVFIAGSYTPFALLAMPPDVGHLVLYIVWGGALAGVLLKVCWPSAPRW
                     VGVPLYLLLGWVAICFTGTILHNAGVTAVVLLAVGGALYSIGGILYAFRWPDPWPATF
                     GYHEFFHACTAVAAICHYVAMWFVVF"
     gene            complement(4166..4549)
                     /locus_tag="VFI55_05160"
     CDS             complement(4166..4549)
                     /locus_tag="VFI55_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7073456.1"
                     /translation="MPSAADRTQAITETVHRYIDLVATGSVDELVELYADDATVEDPV
                     GGEVHIGRRAIHGFYSAVSGVERTCELVSLRVAGHEAAFQFRLTVVTGERRMVIEPID
                     VMVFDHRGKVSAMKSYWSAADVSYL"
     gene            complement(4581..6605)
                     /locus_tag="VFI55_05165"
     CDS             complement(4581..6605)
                     /locus_tag="VFI55_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET7073457.1"
                     /translation="MSPAESAGTNTLGLATSPYLRQHAANPVHWQQWTPQALADATAR
                     DVPILLSVGYAACHWCHVMAHESFDDDEVAAVMNARFVCIKVDREERPDIDAVYMSAT
                     VALTGRGGWPMTCFLTPDGRPFFCGTYYPKGDFLQLLSAVSGTWRDRRGEVEEASDHI
                     AGELRRMSSGLPGLSSGGPQVAPELCDHAVAAVLRDEDGVHGGFGHAPKFPPSAILEA
                     LLRDYERSGSAAALEAVVRTGNAMARGGIYDQLAGGFARYSVDNAWVVPHFEKMLYDN
                     ALLLRAYAHWARRTGDPLARRVAAQTARFLLDGLGTEAEAGMFTSSLDADADGREGST
                     YVWTPEQLTEVLGADDGRWAAAVFGVTRSGTFEHGTSVLQLPADPDDPQRFERVRTAL
                     LAARLTRPQPGRDDKVVTSWNGLAITALAEASVALGEPEFARAARDCAMALLDTHVVD
                     GRLRRASLGGVVGDSAAILEDYAMLATGLLALYQLTAEDSWLTSATELLDTALRHFAD
                     PQRPGCWFDTADDAERLVLRPADPMDGATPSGASSITEALLTAAHLVDGARTERYLRA
                     AGGALRAHSVLLERAPRSAGHWLAVAESAVRGPLQIAVACDPSRSPLLDDARRLAPGG
                     AIVVGGEVDSSPLLLGRDRVRGADAAYVCRGRVCDLPVTRAAELAAALGV"
     gene            complement(6592..6909)
                     /locus_tag="VFI55_05170"
     CDS             complement(6592..6909)
                     /locus_tag="VFI55_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073458.1"
                     /translation="MASAAGWTGWQADAPHNHGPDFGKASPVGLVVVLLLLIATLLLL
                     RSMNRQLKKVPKSFDPEHPEPDQAADEGTDTVDPSADEAPRHAPGQANGSPRAPRPGD
                     EPG"
     gene            complement(6924..7796)
                     /gene="mca"
                     /locus_tag="VFI55_05175"
     CDS             complement(6924..7796)
                     /gene="mca"
                     /locus_tag="VFI55_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HET7073459.1"
                     /translation="MSELRLMAVHAHPDDESSKGAATLARYADEGHRVMVVTLTGGER
                     GDILNPAMDLPDVLEHIAEIRRDEMAKAAEILGVEHTWLGFVDSGLPSGDPPPPLPEG
                     SFALVPLEESVEALVRVVREFRPHVMTTYDENGGYPHPDHIRCHQVSVGAFEAAGDYA
                     CFPDAGEPWTVTKLYYNHGFLRARMQLLHDEFVKRGQEGPFQRWLDHWDPAHDPFESR
                     VTTRVVCSAYFGQRDDALRAHATQIDPNAEFFAAPIAWQERLWPTEEFELARSRVPAK
                     LPEDDLFAGIESDA"
     gene            7930..8364
                     /locus_tag="VFI55_05180"
     CDS             7930..8364
                     /locus_tag="VFI55_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4307 domain-containing protein"
                     /protein_id="HET7073460.1"
                     /translation="MTENPIPRPEARYGRSRLSRVSRRRVAISLAVLAVVVGFVVAVV
                     GYQRLGTSDVAGSLAGYQVIDDETASVTISVTRSDPSRPVDCIVRVRAKDGSETGRRE
                     VLVGPSSQGTVQVTTTVKSSKPPAMADIYGCGADVPGYLRPT"
     gene            8583..9077
                     /gene="greA"
                     /locus_tag="VFI55_05185"
     CDS             8583..9077
                     /gene="greA"
                     /locus_tag="VFI55_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET7073461.1"
                     /translation="MTDTQVTWLTQESHDRLKAELDQLIANRPVIAAEINDRREEGDL
                     RENGGYHAAREEQGQQEARIRQLQDLLNNAKVGEAPKQSGVALPGSVVKVYYNGDKSD
                     SETFLIATRQEGVNDGKLEVYSPNSPLGGALIDAKVGETRRYLVPNGNTVEVTLVSAE
                     PYHS"
     gene            complement(9130..10326)
                     /locus_tag="VFI55_05190"
     CDS             complement(9130..10326)
                     /locus_tag="VFI55_05190"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET7073462.1"
                     /translation="MTKHRQEPEDHTEHRGVTGLATKAIHAGFRPDPATGAVNAPIYA
                     SSTFAQDGVGGLRGGFEYARTGNPTRAALEASLAVLEDGAFGRAFSSGMAATDCALRA
                     MLRPGDHVVIPSDAYGGTFRLIDKVFTQWNVGYTPVALSDLDAVRAAITPQTRLVWVE
                     TPTNPLLSIADIGAIAEVAAEAGKQRAVKVLVDNTFASPALQQPLALGADVVLHSTTK
                     YIGGHSDVVGGALITNDQELDDAFAFLQNGAGAVPGPFDAYLTMRGLKTLVLRMQRHS
                     ENALAVAEFLTAQPSVRSVLYPGLASHPGHQVAARQMRGFGGMVSVRMAGGREAAEKL
                     CARTRVFILAESLGGVESLIEHPSAMTHASTAGSQLEVPDDLVRLSVGIEDIADLLAD
                     LEQALG"
     gene            complement(10422..11156)
                     /locus_tag="VFI55_05195"
     CDS             complement(10422..11156)
                     /locus_tag="VFI55_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019284015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET7073463.1"
                     /translation="MTNPPPPGESPAPGSAGGQQPSSGGYEPGAASSAPGSAFPPPPS
                     AVPGGSSYPPPPLPGGGSYPPPPPSAGGYAPPPPGPAIKTLPTESYASWITRVAAFII
                     DILPYAVVHGIGYGILLATQQSSCVTDITQYAVNQYCVIQNSTIGWVAHAVALVVGLA
                     YLIWDYGYRQGTTGSSLGKSVTKVKVVSEVTGQPIGFGMSLVRQVAHFIDAVICFIGF
                     LFPLWDAKRQTLADKIMTTVVLPTSD"
     gene            complement(11268..12662)
                     /locus_tag="VFI55_05200"
     CDS             complement(11268..12662)
                     /locus_tag="VFI55_05200"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HET7073464.1"
                     /translation="MRIAQHISDLIGGTPLVRLNSVVPDGAATVAAKVEYLNPGGSAK
                     DRIAVRMIDAAEASGQLRAGGTIVEPTSGNTGVGLALVAQHRGYKCVFVCPDKVSEDK
                     RNVLLAYGAEVVVCPTAVPPDHPDSYYSVSDRLVTEIDGAWKPDQYANPEGPASHYAT
                     TGPEIWADTDGKVTHFVAGIGTGGTITGAGRYLKEVSDGRVRVIGADPEGSVYSGGSG
                     RPYLVEGVGEDFWPQAYDRTVPDQIIAVSDSDSFNMTRRLAREEAMLVGGSCGMAVVA
                     AVRVAEEAGPDAVVVVLLPDGGRGYMSKIFNDAWMSSYGFLRSRLDGSTEQPTVGDVL
                     RRKSGALPDLVHTHPSETVRDAIGILREYGVSQMPVVGAEPPVMAGEVAGSVSERELL
                     SAVFEGRAKLADAVAQHMSPPLPMIGAGELVSAAGKALRDWDALMVVEEGKPVGVITR
                     YDLLGFLSEGPRRR"
     gene            complement(12739..13818)
                     /locus_tag="VFI55_05205"
     CDS             complement(12739..13818)
                     /locus_tag="VFI55_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7073465.1"
                     /translation="MTAPSKVSGSPRRAVCRNDILKARRLGARRFAISDGAPVEVLES
                     GPSVAARVATLASRLTIRPILTAGSYVPNLPWPWGLIDLASRVLLPVSATVRETVDLP
                     NASAQLVRAPGVLPADGSRRMVLYFHGGAFLTCGANSHGRLVEALSKFADSPVLVVNY
                     RLLPKHSIGMALDDCRDAYQWLRLRGYEPDQIVLAGDSAGGYLALALAQRLQEEGRGD
                     GEVAAALVAISPLLQLAKECKQAHPNIKSDAMFPAKAFDVLIELVAAAAERNIVDGKP
                     EEIYEPLEHIKPGLPRTLIHVSGSEVLLHDAKLAATRLAAVGVPAEVRVWPGQVHDFQ
                     LAAPLVPEAIRSLWQIGEYIREATG"
     gene            14062..15030
                     /locus_tag="VFI55_05210"
     CDS             14062..15030
                     /locus_tag="VFI55_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET7073466.1"
                     /translation="MPRRSAIALATAGALASTGTAYLGARNLLVGQATHARTVIPKAW
                     DIPPRADGVYTPSGGPVQRWHRGTHIDLHLMIFGDSTATGYGCMSAEEVPGVLIARGL
                     AARTGKVVRLSTKAIVGATSKGVSGQVDAMFVAGSPPDAAVLMLGANDVTALNGISQS
                     AQRLGRAVRKLRTRGAVVVVGTCPDLGVVTAIPQPLRSLVHARTSQLARAQAGAVRAA
                     GGIPVPLAQLLAPQFRAQNELMFSADGFHPSSAAYALAADALLGALAEALSENIETAA
                     PKLPEAPEVALPPAGAPELGEGPTRLSVMSRLWRRPQHGLPAPSSS"
     gene            15123..16340
                     /locus_tag="VFI55_05215"
     CDS             15123..16340
                     /locus_tag="VFI55_05215"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET7073467.1"
                     /translation="MPEAVIVSTARSPIGRAMKGSLASMRPDDLAVQMVRAALDKVPA
                     LNPHQIDDLILGCGLPGGEQGFNIGRVIAVELGYDFLPGTTVNRYCSSSLQTTRMAFH
                     AIKAGEGDAFISAGVETVSRFVKGSSDAWPDTQNPLFNEAQERSVAAAAGADEWHDPR
                     TDGKLPDVYIAMGQTAENVAIISGISREDQDRWGARSQNRAVEAIKSGFFEREITPVT
                     LPDGTTVSADDGPRPGTTYEKLAELKPVFRPNGTVTAGNACPLNDGAAALVITSDTKA
                     KELGLTPLARIVSTGVSGLSPEIMGLGPVEACKMALARAGLSVGDIDLFEINEAFAVQ
                     VLGSARELGIDEDKLNVSGGAIALGHPFGMTGARIATTLLNNLQTHDKTFGLETMCVG
                     GGQGMAMVFERLS"
     gene            complement(16417..17265)
                     /locus_tag="VFI55_05220"
     CDS             complement(16417..17265)
                     /locus_tag="VFI55_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HET7073468.1"
                     /translation="MRETSNPVFRSLPKQRGGYAQFGTGPAQTQGYQADPYFAPYQDT
                     REVRASRPLTIDDVVTKTGMTLAVLVASAVVSYFLVASDHALAMPLTLVGAFGGLGLV
                     LIATFGRKQNSPAIVLSYAVLEGLFLGAISFALANFTVSSASAGALIGEAVMGTLGVF
                     FGMLIVYKTGAIRVTPKFTRMLVAALFGVLALMLGNFVLAMFNVGGGEGLGLRSPGPI
                     GIIFSLVCIGLAAFSFLIDFDAADQMIRAGAPEKAAWGIALGLTVTLVWLYIEILRLL
                     SYLQND"
     gene            17526..18599
                     /locus_tag="VFI55_05225"
     CDS             17526..18599
                     /locus_tag="VFI55_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET7073469.1"
                     /translation="MAREARETGETGESDDVLTCVDGAVGLITLNRPKAINSLNQHMV
                     DALRAVLTRWEKDDAVRAVVLSGAGERGLCAGGDVVAVYQSARKDGVETRRFWRAEYL
                     LNGQIGRFGKPYVSLMDGIVMGGGVGVSAHANTRVVTDTSKVAMPEVGIGFIPDVGGA
                     FLLSRAPGALGLHAALTGAPFSGSDAIAMGFADHYVPHSELGSFTAAIVADGVESALA
                     RHAVEPPPSALAAQRDWIDECFAGDTAEDIVAALRARKGSDAGRASDAADLIATRSPI
                     AVSVALAAVRRAAKLQTLEDVLIQDYRVSSASSRSHDLVEGIRAQIIDKDRNPKWSPA
                     TLAAVTAADIDAYFVPVDDDLNF"
     gene            18612..19391
                     /locus_tag="VFI55_05230"
     CDS             18612..19391
                     /locus_tag="VFI55_05230"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET7073470.1"
                     /translation="MTSTYETILVERDHRVGIITLNRPNALNALNSQVMNEVTGAAAE
                     LDNDPGIGAIVITGSAKAFAAGADIKEMADLTFADAFAADFFVQWSKLAAVRTPTIAA
                     VAGYALGGGCELAMMCDLLIAADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDL
                     ILTGRTIDAAEAERSGLVSRVVPADDLINEAMAVATTISQMSLSATRMAKDAVNRAFE
                     TTLSEGLLYERRLFFSTFATDDQSEGMAAFIEKRPPNFTHR"
     gene            19401..21188
                     /locus_tag="VFI55_05235"
     CDS             19401..21188
                     /locus_tag="VFI55_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015292657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HET7073471.1"
                     /translation="MSEPGSMKTAEGDEPAAPTAESGALTGGAETAAEAKPAPAPPPA
                     RRQWWIRHYTFTGTAVGLLFIWFSMTPSLLPRGPLFQGLVSGFSGAIGYGIGVFCVWL
                     VKYMRSAQSSPPPPRIAWMILIPVGVVGMVGMAIWFHVWQDQVRDLMGVEHLVWWDYP
                     LTAVLSLVVLAAVVEVGQLIRRLVIFLVGKVDRVAPFRLSAAIVVIALLVLTIALLNG
                     VVIKVTMDKLNSTFNQVNEEGKAESAAPKSRLRSGGPESLVPWDTLGHQGRIFVAAGP
                     SVAQLGEFNGAPATEPIRAYAGLNSADGIAATAELAAAELERTGGLQRAVVAVATTTG
                     TGWINEAEADSLEYMYNGNTAIVSMQYSFLPSWLSFLVDKENARRAGQALFEAVDKRI
                     KQLPEGQRPKLVVFGESLGSFGGEAPFMSLNNVLARTDGALFSGPTFNNTVWLQLTDT
                     RDPGSPAWLPIYDDGQNVRFVARQSNLGRPPAAWGHPRVVYLQHASDPIAWWTPDLLF
                     KSPDWLNEKRGYDVLPQTHWIPLVTFLQVSADMAVAVDVPDGHGHHYVADVANGWADV
                     LSPPGWTPDKTAKLRPLLHSDAVISGPSG"
     gene            complement(21189..21644)
                     /locus_tag="VFI55_05240"
     CDS             complement(21189..21644)
                     /locus_tag="VFI55_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7073472.1"
                     /translation="MSRIDLVLRAARRRLRRLRAVEVPDAVSRGAILVDIRPRDQRLR
                     EGELPGALVIERNVLEWRCDPTSEAKVPEAVGDDVEWVIVCSQGYTSSLAAAALLDIG
                     LHRATDAIGGYQALADAGVLNQLHTAARRRSAPPDALHADLAMSERRWI"
     gene            complement(21641..22261)
                     /locus_tag="VFI55_05245"
     CDS             complement(21641..22261)
                     /locus_tag="VFI55_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase"
                     /protein_id="HET7073473.1"
                     /translation="MSLPIAAPVAAPLPLRRLTVSSPSSGPTRLRVPDLLQATDRAAD
                     DVLSGRCDQLLPAGGVPDSRRWFTRIHGDDELDIWLISWVPGHPTELHDHGGSLGALT
                     VLSGSLNEFRWDGRGLRRRRLDAGDQAGFPLGWVHDVVWAPRPVPTPATPVSWPASTV
                     KVPVQPTLSVHAYSPPLTAMSYYEVTDRNRLRRQRTELTDQPEGSQ"
     gene            22373..>22797
                     /locus_tag="VFI55_05250"
     CDS             22373..>22797
                     /locus_tag="VFI55_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LpqV"
                     /protein_id="HET7073474.1"
                     /translation="MRWSSHRPLLWGAVVLATGLSAVTGCSDGAPTSTTGSPSAPSSA
                     RPADIPTGPSSAPPSAVGLSPAGVTTKIDVPADSTEEEYYQACHAAKLWMEAQPKTGA
                     SLVEPYLAMLQASPSGNAGSWNARWSDLTLARQAAVITAA"
BASE COUNT         3776 a         7801 c         7689 g         3531 t
ORIGIN      
        1 gacatcgagc agtggtacgt gtcgcggttc ttgtcgctgc gcggcaccgc gttcgccgac
       61 ccggaatcac acttccacca ttactcggcc ctcgacgacg aaaaggccgt cgttgccgcc
      121 cgcgagatct ggcggtcgat caaccggccg aacctggtcg agaacatcct gccgacccgt
      181 ccccgcgcca ccctggtgct gcgtaaggac gccgatcact cgatcaaccg attgcggctc
      241 cgcaagctgt agcaacgctg ctgggagcta aaacgccggg gcggggcaac ggtccgcaac
      301 gtgcggcggc gccaccccgc gccggcgggt ggggaaacta cgcagcgagg cgacgcactc
      361 cccagtactg cagtccggcg aacgccgccg tgtacacgcc gctggccagc gtcgcggcga
      421 ccccggttgc ggtcatgggc acgactccgg cggccacgat ggcagccagc gtgtagacga
      481 tgttggccgc gacgacgccg atcccgacac ggcgcaagtt gggcaacgcc gcgaggctga
      541 acaccgccag gccgtagagc acgaagaacg cgcccaaagc gtattcctgg ccggacgtca
      601 ggccggtcag ggacgacagc gggtcggcga cgaaggcggc ggccaacccg cacagtcccg
      661 tcaacgtggc atcggcacgc atggcgaaac gcagcagcga atcggtcgag tcgtataggg
      721 gtcgggttgc gagaccagat gcaccagaaa cagaggtcat gtgattactc cttgcgatgg
      781 cggattgggt cgaacagttt tgagcgtgca gcccaagcac tgccatatcg actcgcggca
      841 ctgccaacta ctgccacgga tacaaagacc aggcatttcg tccggctatt cccgaacggg
      901 ccaaattgct acgcgtcagc gatgccgagg tcggctgccg ccgtgatcgt tcgggcaggc
      961 cgaggccgag gggatcttgg gagtggtcac cgggccgaag cttggtcagt ggggacgggg
     1021 acacggcccc gcagatcggc ggcgatgagc cgggccgcgg cgttttgcca gttgtgcagc
     1081 gaccgctgtg ggacttcggt gaccaaccat tgccaggcct gccgggcggt gggatccagg
     1141 ccggcggcgg tggcgttctg cgcatacgcg cgcacaccga cgacgtaggg gaaatacagc
     1201 gagttgtaga aacgccactc ctcagtggtt ccgaaatcgc cgccgtcgcg cggtttgagc
     1261 cgcgcgatgc cgtcggcgag gacggccttg agctcgttgg cccgctccac cggatgatcg
     1321 ggcgcaccac gcgcggccag ccgctcgtcg atcacgggca acgcggtgag cgggctggcg
     1381 accagtttgg tcaggtccgc gtagtggccc agcgctcggc gggtgagcct ggtgaacgtt
     1441 tcgtcgtcca cgtcgtgaat atcccggcgg ggatcgaccg aacgcagcgg caacgcggct
     1501 tcggtgtgcc gcagcgcggc ccggtcggcc cgcagcgtgg gcgatttcga gaacgccatc
     1561 cggtccagca ccccggccag cggatcggcc agcacctgca ccatgatcgc gatcgccagg
     1621 gtggtgaaca gcagcacggt cagcacggtg tgcgcagcgg gctcgtcccg ggtgagggcc
     1681 aggccgatga gcacctggcc gccgaacagc gacgccacca ccgaacatcc ggcgaacgag
     1741 cgcagcatgt cggcgcgcag cgcctggccc tcgtcgaagg cgtcccatag ggccaccgcc
     1801 acgcccagca gcagcaggtc gaaaccggtg gacgccagcg ccagccagct gggcaacagg
     1861 cccagcggga tgatcaggat cgcgttgccc agcgcgaaga acagggtcgc gacgatgatg
     1921 agccccatac cgcgccggcc agcaggcacc ggccgcgccg accgccgcag tgcgacgaac
     1981 atcgccccga gcgtggatac cgagatcacc gcgaacatca ccatgtggcc cgcccgcaac
     2041 ggcccgtcga cgctgccggc cagcgccgcg ccgagcaggg tgagcgcgcc gacgccgccg
     2101 atgaaaagca attcaccggt gcgtgcccgc cagtggtcgc agggccggga cagctcgacc
     2161 agcaccgcga accacgccac gctggggacg gcaaccagat agatctcgac cccgctgagc
     2221 agcgcggcgt gcgcctgaaa tgtgtgagag ccggcggtgc gcaccgcgtc caaggccacc
     2281 accagcgcga agccgcagag cccgaccgcc gccaacacga gcaccggctt gcgcggatcg
     2341 cgggccagca ggtacagccc cagccacccg ctcagcgtga acaccaccgc cgacagcgca
     2401 gccatacatc cagtgtggca cgcgcccgac ctgcggctaa gaccccggag gctacttccc
     2461 gtacctgcga tgccggtggc tgtaatcgcg cagcgcgcgc agaaaatcga cccggcggaa
     2521 cgccggccag tgcgcttcgg tgaaccacat ctccgagtac gcgctttgcc acagcagaaa
     2581 cccggacagc cgttgctcgc ccgaggtgcg gatcaccagg tcgggatcgg gctgcccgga
     2641 ggtgtacagg ttttccgaga tgccttcgac ggtcaccgcg tcgatgagct cctcggcggt
     2701 cgcgccgttg gcgagttgtt tgctcaacaa cgcccgtacc gcgtccacga tctcccggcg
     2761 gccgccgtag ccgacggcga cgttcacgtg gaaaggcgcc accgccgggg tggactccac
     2821 cgcgtcgcgc agccggcggg ccggttcctg gccgatcagc gccaggtccc cgacggtgcg
     2881 cacgctccag cgattggccg gcgcgcagat ctcctcgacc acatcggtga tgatctcgat
     2941 gagggcggcc agctcgtcgg ggtcacgccg caggttttcg gccgagagca ggtagacggt
     3001 ggtcatttcg atgccggctt cctggcacca gcgcagcatt tcggcgatct tggcggcacc
     3061 catccggtag ccgtagctga cgtcgtcgta acccgcgtcg cgcgcccagc gccggttccc
     3121 gtcgcacagg accgcgatgt ggcggggaag ttgggatttc gaggcggcca gaccctgccg
     3181 aagccgcatc tcgtagacgc ggtacaacgg ctccttgagc cgcggcggga taatctccac
     3241 gaaaatccac actagagcga caacgaattc cggcctgcgt ttccacccgg aattccagcg
     3301 agcacagcgt ttgcgacggc gtgtcgcggc cgttccccgc tactgttgac ggagagcggc
     3361 cccgggagtg tcccggcctg gacgacaagg caacaggaga catgacccag accagcacgg
     3421 ccagccatgc ggagcccgag tcgccgaacc tcacggccaa caccgcgcac cagctcgtcg
     3481 acggcgtcgc gagggtactg accaagccgc gtttccgcgg ctggattcac gtctactccg
     3541 ccggcgtcgc cgtcgcggcc ggcgcgtcct tgatcgcggt gtcgtgggcg gtcggatcca
     3601 cccgcgccgg acttgccacc ttcgcctata ccgcggccac gatcctgatg ttcaccgtca
     3661 gcgccaccta ccaccgcgtc cactggaagt ccgccaccaa gcagaagtgg atgaagcggc
     3721 tcgaccattc gatgatcttc gtgttcatcg cgggcagtta tacgccgttc gcgctgctgg
     3781 cgatgccacc cgacgtggga cacctggtgc tctacatcgt gtggggtggt gcgctggccg
     3841 gggtcttgct gaaggtgtgc tggccgtcgg cgccgcggtg ggtgggtgtg ccgctctacc
     3901 tgctgctggg ctgggtggcg atctgcttca ccggaaccat cctgcacaac gcgggggtga
     3961 ccgccgttgt gttgctggcg gtcggtggcg ccttgtacag catcggcggc atcctctatg
     4021 catttcgctg gccagacccg tggcccgcga cgttcggcta ccacgagttc tttcacgcgt
     4081 gcaccgcggt agcggcgatc tgccactacg tcgcgatgtg gttcgtggtc ttctagccgg
     4141 tgcgcgaaag cgccgcggcg aatcgctaca ggtagctcac gtcggccgct gaccagtagg
     4201 acttcatcgc gctgaccttg ccccggtggt cgaataccat cacgtcgatc ggctcgatca
     4261 ccatccggcg ctcgcccgtg accacggtga gccggaactg gaacgccgcc tcgtgtccgg
     4321 ccacccgcag cgacaccagc tcgcacgtcc gctctacacc gctgaccgcg gagtagaagc
     4381 cgtggatcgc ccggcgaccg atgtgcacct cgccgccgac cgggtcctcg acggtggcgt
     4441 cgtcggcgta caactcgacc aattcgtcga cgctgcccgt ggcgaccaga tcgatgtagc
     4501 ggtgcaccgt ctcggtgatc gcctgggtcc tgtcggcggc actgggcatg agacgcaggg
     4561 tagcctccgg gctgtcacct tcagacaccc agggcggccg ccagttcggc tgccctggtt
     4621 acgggcaggt cgcagacccg gccgcggcac acgtaggccg cgtcggcacc gcggacacgg
     4681 tcccggccca gcagcagcgg cgacgagtcc acctcgccgc ccacgacgat cgccccgccc
     4741 ggcgccagcc ggcgcgcgtc gtcgagcagc ggcgaccgcg acggatcgca ggcgaccgcg
     4801 atctgcagcg gtccgcgcac cgcggattcg gcgacggcca gccaatgccc ggccgagcgt
     4861 ggcgcgcgct ccagcagcac cgaatgcgcg cggagcgccc cgcccgccgc ccgcagatac
     4921 cgctccgtgc gcgcaccgtc gaccagatgc gccgcggtca gcagcgcttc ggtgatcgac
     4981 gacgcgcccg acggcgtcgc cccgtccatc gggtcggcgg gccgcagcac caaccgttcg
     5041 gcgtcgtcgg ccgtatcgaa ccagcagccc ggccgctgcg ggtctgcgaa gtgccgcagc
     5101 gcggtgtcca gcaactcggt cgccgacgtc agccaggaat cctcggcggt cagctggtac
     5161 agcgcgagca ggccggtggc cagcatcgcg taatcctcca ggatggcggc actgtcgccg
     5221 accaccccgc cgaggctggc gcgcctcagc cgcccgtcca cgacgtgggt gtccagcagc
     5281 gccatggcgc aatcccgtgc ggcgcgggcg aattcgggtt cgcccaacgc cacactggcc
     5341 tcggccagcg cggtgatcgc cagaccgttc caggacgtga cgaccttgtc gtcgcgtccc
     5401 ggctggggcc gggtgagccg ggcggccagc agcgcggtgc ggacgcgttc gaatcgctgc
     5461 ggatcgtccg gatcggcggg caactgcagc accgaggtcc cgtgttcgaa ggtgccggac
     5521 cgggtgacgc cgaaaaccgc tgccgcccaa cggccgtcgt cggcgccaag cacctcggtc
     5581 agctgctcgg gcgtccagac ataggtcgag ccctcacgac cgtcggcgtc ggcgtccagc
     5641 gacgacgtga acatcccggc ttcggcttcg gtgcccagcc cgtcgagcag aaatcgcgcg
     5701 gtctgcgccg cgacccggcg cgccaacggg tctccggtac gccgagccca gtgtgcatat
     5761 gctcgcagca gcagcgcgtt gtcgtacagc atcttctcga aatgcggtac cacccaggcg
     5821 ttgtcgacgc tgtagcgggc gaagccgccg gcgagttggt cgtagatacc gccccgggcc
     5881 atcgcgttgc cggtgcgtac caccgcctcc agggccgcgg ctgacccgct gcgctcgtag
     5941 tcgcgcagca gcgcctcgag gatcgccgac ggcgggaatt tgggcgcgtg gccgaacccg
     6001 ccgtgcacgc cgtcctcatc gcgcagcact gccgcgacgg cgtggtcgca cagctccggc
     6061 gccacctgcg gacccccaga cgacaagccg ggcagtcccg acgacattct gcgcaattcg
     6121 ccggcgatgt ggtcggaagc ctcctccacc tccccgcggc ggtcccgcca ggtgccggaa
     6181 acggccgaaa gtagttgcag gaaatcgcct ttcgggtagt acgtgccgca gaagaagggc
     6241 cggccgtcgg gcgtcaggaa gcacgtcatc ggccaaccgc cgcggccggt gagcgcgacg
     6301 gtcgcgctca tgtagaccgc gtcgatgtcc ggccgttcct cgcggtcgac cttgatgcac
     6361 acgaatcgcg cgttcatcac ggcggccacc tcgtcgtcgt cgaacgattc gtgggccatg
     6421 acgtgacacc agtggcaggc ggcgtagccg acggacagca ggatcggcac atcgcgcgcg
     6481 gtcgcgtcgg ccagcgcctg cggcgtccac tgctgccaat gcaccggatt ggcggcgtgc
     6541 tggcgcaggt acgggctggt ggccagtccg agcgtgttcg tcccggcaga ctcagccggg
     6601 ctcatcgccg ggtcttggtg ctcgcggcga tccgttcgcc tgaccggggg cgtgcctcgg
     6661 ggcctcgtcc gccgatgggt cgaccgtgtc ggtgccctcg tcggcggcct gatccggctc
     6721 ggggtgctcg gggtcgaacg atttgggcac cttcttcagt tgccgattca tcgatcgcag
     6781 caacaacagt gtggcgatca acagcagcag gacgaccacc agccccaccg ggctggcctt
     6841 gccgaaatcg ggcccgtgat tgtgcggcgc gtcagcctgc cagccggtcc acccggcggc
     6901 actcgccaca aggaggtggt aattcacgcg tcactctcga ttccggcgaa cagatcgtcc
     6961 tcgggcagtt tggcgggcac ccgggaacgc gccaactcga attcctcggt gggccacagt
     7021 cgctcctgcc aggcgatcgg agcggcgaag aactcggcgt tggggtcgat ctgcgtggcg
     7081 tgcgcccgca gggcatcgtc gcgctggccg aaatacgccg agcacaccac gcgcgtcgtc
     7141 acccgggact cgaacgggtc atgagcggga tcccagtggt caagccacct ttgaaacggc
     7201 ccttcttggc cgcgcttgac gaactcgtcg tgcagcagtt gcatccgcgc ccgcaggaag
     7261 ccgtggttgt agtacagctt ggtcaccgtc cagggctcac cggcgtccgg gaagcaggca
     7321 tagtcgccgg ccgcctcgaa cgcgccgacc gaaacctggt ggcagcgaat gtgatcggga
     7381 tgcgggtagc cgccgttctc gtcgtaggtg gtcatcacgt gcggccggaa ctcgcggacc
     7441 acccgcacca gcgcctcgac ggactcctcc agcggcacca gcgcgaaact gccctccggc
     7501 agtggcggtg gcgggtcacc gctgggcaaa ccggagtcga cgaaaccgag ccaggtgtgc
     7561 tccacgccaa ggatctcggc ggccttcgcc atctcgtcac ggcggatttc ggcgatgtgc
     7621 tcgagcacat ccggcaggtc catcgccggg ttgagaatgt cgccgcgctc gcccccggtc
     7681 aacgtcacca ccatgacccg atgaccctcg tcggcgtacc gggcgagggt ggcagcaccc
     7741 ttgctggact cgtcgtcggg gtgcgcgtgc accgccatca accggagttc gctcacgtgc
     7801 ccccttgtcg gatcgctggt cgcccccggg aagggctagc tgagctagaa gcgaccttat
     7861 aattccagtt ccgatcgttg cacgctgtcg gccggcgtcc ggcagcccat cagccaggca
     7921 tacccagaca tgaccgaaaa tccgattccg cgtcccgagg cccgctacgg gcgttcgcga
     7981 ctgtcccgcg tatcgcggcg tcgcgtggcc atcagccttg ccgtgctggc cgttgtcgtg
     8041 ggcttcgtcg tcgccgttgt cggctatcaa cgactcggca ccagcgacgt cgccggctcg
     8101 ctggccggct accaggtgat cgacgacgag acggcgtcag taacgatcag cgtgacgcga
     8161 tccgatccgt cgcggccggt ggactgcatc gtgcgggttc gggccaagga cggcagcgag
     8221 acggggaggc gagaggtgct ggtagggccg tccagccagg gcacggtgca ggtgacgacg
     8281 acggtgaaat ccagcaagcc gcccgcgatg gctgacatct acggctgcgg ggccgacgtg
     8341 cccggctacc tgcgaccgac ctgaccgcac gacgagggcc gaattgcaaa tgtgtgcgtc
     8401 atcagctgtt tgcgcggtgg taacatgggt gaatgcacgg ttcctgctgg gaccgtgtat
     8461 tgctgcatta aggccgtgag cagaacggag cggcataaac ggggtgggac ccgcatactc
     8521 ccggcagtgg ggatcacaaa cttgcgcgga tgcgcggacg agaatacgag gagcgcgacg
     8581 agatgacgga tactcaggtg acctggctga cccaggagtc acatgaccga ctgaaggctg
     8641 agctcgatca gctcatcgcg aatcgtccgg tgatcgccgc cgaaatcaac gaccgccgcg
     8701 aggaggggga cctccgtgaa aacggcggat accacgccgc ccgcgaggag caaggccagc
     8761 aggaggcccg catccgtcag ctgcaggacc tgctcaacaa cgccaaggtc ggcgaggcac
     8821 ccaagcagtc cggcgtcgcg ctgcccggtt cggtggtgaa ggtgtactac aacggcgaca
     8881 agtcggactc ggagaccttc ctgatcgcca cccgccagga gggcgtcaac gacggcaagc
     8941 tcgaggtgta ctcgccgaac tcaccgctcg gtggcgccct gatcgacgcc aaggtcggtg
     9001 agacccgccg ctacttggtg cccaacggca acaccgtcga ggtcaccttg gtcagcgcgg
     9061 agccttacca ctcctgactt ctgccgcgag cagacgcccg ccggccggcg gtgccgcagc
     9121 gcgccctcgt tagcccaggg cttgttcgag atcggccaac aggtcggcga tgtcctcgat
     9181 acccaccgaa agccgcacca ggtcgtcggg cacttccaat tgcgagccag ccgtggacgc
     9241 gtgggtcatg gcgctggggt gctcgatcaa cgactccacc ccgcccagcg actcggccag
     9301 gatgaacacg cgggtgcggg cgcacagctt ttccgcggcc tcgcggccgc ccgccatgcg
     9361 caccgacacc atgccgccga agccgcgcat ctgccgcgcg gcgacctggt gcccgggatg
     9421 gctggccaaa cccggataca gcacgctacg caccgacggc tgcgccgtga ggaattccgc
     9481 tacggccaaa gcgttttcac tgtgtcgctg catgcgcagc accagcgtct tcaggccgcg
     9541 catcgtcagg taggcgtcga acgggcccgg caccgcgccg gccccgttct gcaggaaagc
     9601 gaacgcgtcg tccagctctt ggtcgttggt gatcagcgcg ccacccacca cgtcggagtg
     9661 gccgccgatg tacttggtgg tcgagtgcag caccacgtcg gcgcccagcg ccaacggctg
     9721 ctgcagggcg ggcgacgcga aggtgttgtc caccaacacc tttaccgcac gttgtttgcc
     9781 ggcctcggcc gcgacctcgg cgatggcccc gatgtcggcg atggacagca acggattggt
     9841 cggcgtttcc acccacacca ggcgggtctg cggcgtgatg gcggcgcgca cggcgtccag
     9901 gtcggacaaa gccaccggcg tgtacccgac gttccactgg gtgaacacct tgtcgatgag
     9961 ccggaaggtg ccgccgtagg cgtcgctcgg gatgaccacg tggtcaccgg gccgcagcat
    10021 tgcgcgcagg gcgcagtcgg tggcggccat ccccgaactg aacgcccgcc cgaaggcgcc
    10081 gtcttcgagc accgccagcg aggcctcgag cgcggcccgg gtcgggttgc cggtgcgcgc
    10141 gtactcgaac ccgccgcgca ggccgcccac accgtcttgg gcgaatgtgc tgctggcgta
    10201 gatcggggcg ttcaccgccc cggtcgcggg atccggacgg aagccggcgt gaatggcctt
    10261 ggtggccagt ccggtaaccc cccggtgctc cgtgtggtcc tctggttcct ggcggtgttt
    10321 ggtcatcggc aatcagccta gccagacccc gcagccaacc cccggagtgg tgttttccac
    10381 gcgcgtcatg cggtcgggcc tatcgccgtg gtgccacgct gtcaatccga ggtcggcagg
    10441 acgaccgtcg tcatgatctt gtcggccagg gtctgccgct tggcgtccca gagcgggaac
    10501 aggaaaccga tgaagcaaat caccgcgtcg atgaagtgcg cgacctggcg taccagcgac
    10561 atcccgaagc cgatgggctg gccggtgacc tcgctgacca ccttgacctt cgtcaccgac
    10621 ttgcccaggc tcgaaccggt ggtgccctgg cggtagccgt agtcccagat caggtaggcc
    10681 agccccacca ccagcgccac cgcgtgcgcc acccagccga tcgtcgagtt ctggatcacg
    10741 cagtactggt tgacggcgta ctgcgtgatg tccgtgacgc acgatgactg ctgggtggcg
    10801 agcaggatcc cgtaaccgat cccgtgcacg acggcatacg ggaggatgtc gatgatgaat
    10861 gccgcgaccc gggtgatcca cgacgcgtag gactcggtcg gcagggtttt gatcgccggc
    10921 ccgggcggtg gcggcgcata tccaccggcc gacggcgggg gtggagggta ggaaccgccg
    10981 ccgggcaacg gcggcggagg gtaagaactt ccgcccggca ccgcggaggg cggcggggga
    11041 aaggcgctgc cgggcgccga agaagcggcc ccgggttcgt acccaccgga cgacggctgc
    11101 tgcccaccgg ctgacccagg ggctggagac tcaccgggcg gtggtggatt cgtcatgggc
    11161 aacctttcac tgcggattag ttgaaccagg ccgcattaca gtacctgagg gcccgttttg
    11221 cggggccgtt ccccgagacg cgcgtcacac gcttaacgct cgctgagcta acgtcgccgg
    11281 ggcccttcgg acaggaagcc cagcaggtcg tagcgggtga tgaccccgac cggcttgccc
    11341 tcctccacca ccatcaacgc gtcccagtcc cgcaacgcct tgccggccgc gctgaccaat
    11401 tcgccggcgc cgatcatcgg cagcggcggg ctcatgtgct gcgcgacggc gtcggccagc
    11461 ttggcgcggc cctcgaagac ggcggagagc aactcgcgtt cggagacact gccggcgacc
    11521 tcaccggcca tcaccggcgg ctcggcgccg accaccggca tctgcgatac cccgtattcg
    11581 cgcagaatgc cgatggcgtc gcgcaccgtc tcagacggat gggtgtgcac caggtcgggc
    11641 agcgcacccg atttgcggcg cagcacatcg ccgaccgtgg gctgctcggt cgagccgtcg
    11701 aggcggctgc gcaggaaccc gtaggacgac atccaggcgt cgttgaagat cttcgacatg
    11761 tagccccgcc cgccgtcggg cagcaggacc acgaccaccg cgtcgggtcc ggcttcctcg
    11821 gcgaccctca ccgcggccac caccgccatc ccgcatgacc cgccgaccag catcgcttct
    11881 tcgcgcgcca gccgccgggt catgttgaag gagtcggagt ccgaaaccgc gatgatctgg
    11941 tcgggcaccg tgcggtcgta ggcctgcggc cagaaatcct cgccgacgcc ctcgaccagg
    12001 tagggccggc cgctgccgcc cgaatacacc gagccttcgg ggtcggcgcc gatgacgcgg
    12061 acccgcccgt ccgacacctc cttgaggtag cggcccgcgc ccgtgatcgt cccgccggtg
    12121 ccgatgccgg cgacgaaatg ggtgactttg ccgtcggtgt cggcccagat ctccggaccg
    12181 gtggttgcgt agtggctggc cggtccttcg gggttggcgt actggtcggg tttccaggcg
    12241 ccgtcgattt cggtgacgag cctgtcggag acgctgtagt agctgtccgg atggtcgggc
    12301 ggcaccgccg tcgggcacac caccacctcc gcgccgtagg cgagcagcac gttgcgcttg
    12361 tcctcgctga ccttgtcggg acagacgaac acgcacttgt atccgcggtg ctgtgcgact
    12421 agggcgaggc cgacgccggt gtttcccgac gtgggctcga cgatggtgcc accagctctg
    12481 agctgcccgc tggcttccgc ggcgtcgatc atccgcaccg cgatgcggtc cttcgcgctg
    12541 ccgccggggt tgaggtattc gaccttggcc gccacggtgg ccgcgccgtc ggggacgacg
    12601 gagttcaacc ggaccagcgg agtgccgccg atgaggtcac tgatgtgctg ggcgatccgc
    12661 atgcgttcat cgtctcaggc ggtttccgtc ggtgcacacc cacgctcggt gccacccgaa
    12721 gcccggcgcg tggtcgcgtc agccggtggc ttcgcgaatg tattcgccga tctgccacag
    12781 tgaacggatc gcctccggga ccagcggcgc ggccagctgg aagtcgtgca cctgaccggg
    12841 ccagacccgc acctcggccg gcacgccaac cgcggccagc ctggtcgcgg cgagcttcgc
    12901 gtcgtgcagc aacacttcgg atccggacac gtggatgagc gtccgcggca ggcccggctt
    12961 gatgtgctcc agcggctcgt agatctcttc gggtttgccg tcgacgatgt ttctttcggc
    13021 cgcggcagca accagctcga tgagcacgtc gaacgccttg gcggggaaca tcgcgtcgct
    13081 cttgatgttg ggatgggcct gcttgcattc ctttgccagc tgcagcagtg gcgagatcgc
    13141 taccagcgcc gccgccacct ccccgtcccc gcgcccctcc tcctgcagac gttgcgccag
    13201 ggcgagcgcg aggtacccgc ccgcggagtc accggccagc acgatctggt ccggctcgta
    13261 tccgcgcagg cggagccatt gataggcgtc gcggcagtcg tcgagggcca tcccgatcga
    13321 gtgcttgggc aacagccggt aattcaccac caacacaggt gagtcagcaa acttcgacag
    13381 agcctcgacg agtcgtccgt gcgagtttgc tccgcaggtc aggaaagcgc cgccgtggaa
    13441 gtagagaacc atccgccggc tgccgtccgc cggcagcacc cccggcgcgc gcaccaactg
    13501 tgctgacgcg tttggcaaat ccaccgtctc ccggacggtg gccgacaccg ggagcaggac
    13561 ccgggatgcc aggtcgatca ggccccacgg ccagggcagg ttgggcacgt agctgccggc
    13621 ggtcagaatc ggccggatcg tcagccgtga cgcgagtgtg gcgactcgcg cggcaacgct
    13681 gggccccgat tcgaggacct cgacgggcgc gccatcactg atggcgaacc tgcgggcccc
    13741 aagtctgcgt gccttgagga tgtcattacg gcagacggct ctccgggggg agccggaaac
    13801 cttgctcggt gcggtcatgc tcaacacttc ctacgtcttt gtagtgcgat acagcatgtg
    13861 acgaggaggc caagagcacg gttcatatgt tcgccgagcc attcagccaa cccgttggga
    13921 cttagcttga cagcgaatac taagtacaac gtttgaagaa gctgatttga taccacattt
    13981 gcttcacaac gtgatcactt tgttcgctac ctgtccgcgc aaacagcgaa gcggacctaa
    14041 actgaatgcg tgaccatccg ggtgccccgt cgttcggcga tcgccctggc cacagccggt
    14101 gcactcgcct cgacaggcac ggcctacctg ggcgcacgca acctgctggt cggccaggcg
    14161 acgcacgcgc gcaccgtgat cccgaaggcc tgggacattc cgccccgcgc cgatggtgtg
    14221 tatacgccca gcggcgggcc ggtgcagcgg tggcaccgcg ggacgcacat cgacctgcac
    14281 ctgatgatct tcggggactc gacggccacc ggctatggct gcatgagcgc cgaagaagtt
    14341 ccgggcgtgc tgatcgcacg cgggctagcc gcacggaccg gcaaggtggt ccggctgagc
    14401 accaaggcca tcgtcggcgc cacgtcaaag ggcgtgtccg gccaggtgga cgcgatgttc
    14461 gtggccggat cgccgccgga tgcggccgtg ctgatgctcg gcgccaacga tgtgacggcc
    14521 ctcaacggca tcagccagtc cgcgcagcgg cttggcaggg cggtgcgcaa gttgcgcacc
    14581 cgcggagcgg tggtggtcgt gggcacctgc ccggatctcg gcgtggtcac cgccatcccc
    14641 cagccgcttc gctcgctggt tcacgcgcgg acttcgcagc ttgcccgcgc ccaggccggc
    14701 gccgtccggg cggccggcgg tatacccgtg ccactggcgc agctgctggc gccgcaattc
    14761 cgggcccaga acgagctgat gttctccgcc gacggtttcc atccctcgtc ggccgcgtat
    14821 gcgctggccg cggacgcgct gttgggcgcg ctggccgagg cgctgagcga aaatatcgag
    14881 acggccgcgc ccaagctgcc cgaagcgccc gaggtcgctc tgccgcccgc cggcgcgccg
    14941 gagctcggcg agggtcccac gcgactcagc gtgatgtcgc ggctgtggcg gcgcccccag
    15001 cacgggttgc ccgccccgtc ctcgtcctga tcttttggca cgactagatt tgttctggct
    15061 cgtcggggtg gacataccac ccaccaatga agcgagttct catcccatcg gagggagtcc
    15121 tcatgcctga agccgtcatc gtctcaactg cccgctcgcc aattggccgc gccatgaagg
    15181 ggtcgctggc cagcatgcgg cccgacgacc tcgccgtcca gatggtgcgt gccgcgctcg
    15241 acaaggtacc cgcgctgaac ccgcaccaga tcgacgacct catcctgggc tgcggcctgc
    15301 cgggcggcga gcagggcttc aatatcggcc gggtgatcgc cgtcgagctg ggctatgact
    15361 tcctgccggg taccacggtc aaccggtatt gctcgtcgtc gctgcagacc acccggatgg
    15421 ccttccacgc gatcaaggcg ggcgaaggcg acgcgttcat ctccgccggg gtggagaccg
    15481 tgtcccggtt cgtcaaaggc agctccgacg cttggccgga cacccagaac ccgctgttca
    15541 acgaggccca ggagcgttcg gtcgcggcgg ccgccggggc cgacgaatgg cacgacccgc
    15601 gcaccgacgg gaagctgccc gacgtctaca tcgcgatggg tcagaccgcc gagaacgtcg
    15661 ccatcatcag cggcatcagc cgcgaagacc aggaccgctg gggcgcgcgc agccagaacc
    15721 gtgccgtgga ggcgatcaag agcgggttct tcgagcgcga gatcactccg gtgaccctgc
    15781 ccgacggcac caccgtgagc gccgacgacg gcccccgtcc gggcaccacc tacgagaagc
    15841 tggccgagct gaagccggtg ttccggccca acggcaccgt gacggcgggg aacgcctgcc
    15901 cgctcaacga cggcgccgcc gcgctggtga tcaccagcga taccaaagcc aaggaactgg
    15961 gcctgacacc gctagcccgc atcgtgtcca ccggcgtcag cggcctctcg ccggagatca
    16021 tggggctggg cccggtcgag gcgtgcaaga tggcgctcgc cagggccggc ttgtcggtcg
    16081 gtgacatcga cctgttcgag atcaacgagg cgttcgcggt gcaggtgctg ggctcggccc
    16141 gcgagctggg catcgacgag gacaagctga acgtgtccgg cggggcgatc gcgctgggcc
    16201 accccttcgg catgaccggc gcgcgcatcg ccaccacgct gttgaacaac ctgcagaccc
    16261 acgacaagac gttcggcctg gagaccatgt gtgtcggcgg cggccagggc atggcgatgg
    16321 tgttcgagcg gctgagctga acgccgcacc cgccgcacag caacaaaaaa ccggcacgcc
    16381 acctggcgtg ccggtttttt cccgtcggcg tccgtgttag tcgttttgca gataactgag
    16441 cagacgcaag atctcgatgt agagccagac cagagtgacg gtgaggccca aagcgatgcc
    16501 ccaagccgcc ttctccggcg ctccggcgcg gatcatctgg tcggccgcgt cgaagtcgat
    16561 caggaagctg aacgccgcca gcccgataca cacgagcgag aagatgatcc cgatgggccc
    16621 cgggctgcgc aggcccaggc cttcgccgcc acccacgttg aacatcgcca gcacgaagtt
    16681 accgagcatc agggccagca caccgaacag tgccgcgacc agcatgcggg tgaacttggg
    16741 cgtgacccga atggcgcccg tcttgtagac gatcagcatt ccgaagaaca ccccgagggt
    16801 gcccatcact gcctcaccga tcagcgcgcc ggcgctggcg gaagacaccg tgaagttcgc
    16861 cagcgcgaag gagatggcgc cgaggaacag gccctcgagc accgcgtagc tgagcacgat
    16921 cgccgggctg ttctgcttgc gaccgaaggt cgcgatcagc accagcccca agccgccgaa
    16981 cgcaccgacg agggtcaggg gcatggcgag cgcgtgatcg gacgccacca ggaagtagga
    17041 cacgacggcc gaggcgacca gcaccgccag cgtcatgccg gtcttcgtga cgacatcgtc
    17101 gatggtcagc ggacgggagg cccggacctc gcgggtgtcc tggtagggag cgaaataggg
    17161 gtcggcctgg tacccctgcg tctgggctgg gccagtgccg aattgcgcgt atccgcccct
    17221 ctgctttggc agcgaacgaa ataccgggtt acttgtctcc cgcaccgtcg gatcctctct
    17281 tatgactgtg gcttcgagct gtgcgaacat tcgctcaacg atcagcggac cgcccgggtt
    17341 cccagcggac ctgctgtttt ctgggtattt ccggcttgtt tacgaactct gccgtggtcg
    17401 tccggtcaaa cataaacctt acccgcggat actgcttcgc cgttaacgat ctagattgct
    17461 cgtcgaggcg caacagcctt gtcggcagct ctggacacga atgccgtttg aagggaggcg
    17521 agggcgtggc gagggaagcc agggagactg gcgaaactgg ggaatccgat gacgtcctga
    17581 cctgcgtcga cggtgccgtc ggcttgatca cgctcaaccg ccccaaggcg atcaactcgc
    17641 tcaatcagca catggtcgac gcgttgcgcg ccgtgctcac ccgctgggaa aaggacgacg
    17701 ccgtgcgtgc ggtggtgttg tccggggccg gcgagcgcgg attgtgtgcc ggcggcgatg
    17761 tggtcgcggt ctaccagagc gcgcgcaagg acggggtcga gacccggcgg ttctggcgcg
    17821 ccgagtatct gctcaacggg cagatcgggc ggttcggcaa accgtacgtg tcgttgatgg
    17881 acggcatcgt gatgggcggc ggtgtcggtg tcagtgcgca cgcgaacacc agggtggtga
    17941 ccgatacctc gaaagtcgcg atgcccgagg tgggaatcgg cttcatcccc gacgtgggcg
    18001 gggcgttcct gttgtcccgg gcgccgggcg cgctggggct gcacgccgcc ctgaccggtg
    18061 cgccgttttc cggatccgat gccatcgcga tggggttcgc cgaccactac gtgccgcaca
    18121 gcgaactcgg ctcgttcacc gccgcgatcg tcgccgacgg cgtcgagagc gcactggccc
    18181 gtcatgccgt cgaacctccg ccgagtgccc ttgcggcaca acgtgattgg atcgacgaat
    18241 gctttgcggg tgacaccgcc gaagacatcg tcgccgcgct gcgcgcccgt aagggttccg
    18301 acgccggccg ggccagcgac gccgccgacc tgatcgccac ccgctccccc atcgcggtgt
    18361 cggtggcgtt ggcagcggta cgtcgcgcgg ccaagctgca aacgctggaa gatgttctga
    18421 tccaggacta tcgggtgtcg agcgcatcgt cgcgctcaca cgatttggtg gagggtatcc
    18481 gggcgcagat catcgacaaa gatcgcaatc cgaagtggtc tccggcgacg ctggcagcgg
    18541 tcaccgcggc cgacatcgac gcgtatttcg tgccggtcga cgacgacttg aatttctaga
    18601 aaggcggttc agtgacatcc acgtacgaaa ccatcctggt cgagcgcgac caccgagtcg
    18661 gcatcatcac cctgaaccgc cctaatgcgc tcaacgcgct caacagccag gtgatgaacg
    18721 aagtcaccgg cgccgcagcc gaactggaca acgacccggg catcggggcg atcgtcatca
    18781 ccggttcggc caaggccttc gccgccggcg ccgacatcaa ggaaatggcc gacctcacgt
    18841 tcgccgacgc gttcgccgcc gacttctttg tccagtggag caagctggcc gcggtccgca
    18901 ccccgacgat cgccgcggtg gccggctacg cgctgggcgg tggttgcgag cttgcgatga
    18961 tgtgcgacct gttgatcgcg gccgacacgg cgaagttcgg ccagccggag atcaaactcg
    19021 gcgtgctgcc cggcatgggc ggctcacagc gcctgacccg ggccatcggc aaggccaagg
    19081 cgatggacct catcttgacc gggcgcacca tcgacgccgc cgaggccgaa cgcagcggcc
    19141 tggtgtcccg ggtggtgccg gccgacgacc tgatcaacga ggccatggcg gtcgccacca
    19201 ccatttcgca gatgtcgctg tcggccaccc gaatggccaa ggacgccgtc aaccgtgcct
    19261 tcgaaaccac cctgtccgaa ggactcctgt acgaacgccg gctcttcttc tcgaccttcg
    19321 cgaccgacga ccagtccgaa ggtatggccg cattcatcga gaagcgccca ccgaatttca
    19381 cccaccgata ggacgcatcg atgagcgaac cgggttccat gaagaccgcc gagggcgacg
    19441 aaccggctgc cccgaccgca gagtctggcg cgctcaccgg cggcgcggag accgcggccg
    19501 aggccaagcc cgcccccgca ccaccgccgg cccgccgcca gtggtggatt cggcactaca
    19561 cgttcaccgg caccgcggtc ggcctgctgt tcatctggtt ttccatgacg ccgtcgctgc
    19621 tgccgcgcgg cccgttgttc caggggttgg tgagcgggtt ttccggcgcg atcggctacg
    19681 gcatcggcgt cttctgcgta tggctggtca agtacatgcg ctcggcgcaa tccagcccac
    19741 cgccgccgcg catcgcgtgg atgattctca tcccggtcgg cgtggtcggc atggtcggca
    19801 tggccatctg gttccacgtc tggcaagacc aggtgcgcga cctgatgggc gtcgagcacc
    19861 tggtgtggtg ggactacccg ctgaccgcgg tcctgtcgct ggtggtgctg gccgcggtgg
    19921 tcgaagtcgg ccagctgatc cggcggctgg tcatcttcct ggtcgggaaa gtcgaccggg
    19981 tcgcgccgtt ccggctgtcg gcggccatcg tcgtgatcgc gctcctggtg ctgacgatcg
    20041 cgctgctcaa cggcgtcgtc atcaaagtca ccatggacaa attgaacagc accttcaacc
    20101 aggtgaacga ggaaggcaag gccgagagcg cggcgccgaa atcccggctg cgatccggtg
    20161 gcccggagtc gctggtgcca tgggacacgc tgggccatca gggccgcatc ttcgtggcgg
    20221 ccggtccgag cgtcgctcaa ctcggcgaat tcaacggcgc cccggccacc gaaccgatcc
    20281 gggcctacgc cgggctgaat tccgccgacg ggatcgcggc gaccgccgaa ctggccgcgg
    20341 cggaattgga gcgcaccggc ggactgcaac gcgccgtcgt cgcggtcgcc accaccaccg
    20401 gtaccggctg gatcaacgag gccgaggccg actcgctgga gtacatgtac aacggcaaca
    20461 ccgcgatcgt gagcatgcag tattcgtttc tgcccagctg gctgtcgttc ctggtcgaca
    20521 aggagaacgc ccgccgcgcc ggtcaggcgc tgttcgaggc ggtcgacaaa cggatcaagc
    20581 agctgccgga agggcagcgc ccgaagctgg tcgttttcgg cgagagtctg ggatcgttcg
    20641 gcggcgaggc gccgttcatg agcctcaaca acgtcctcgc ccgcaccgac ggtgcgctgt
    20701 tcagcgggcc gacgttcaac aacaccgtgt ggctccagct gacggacacc cgcgacccgg
    20761 gttctccggc gtggctgccg atctacgacg acggtcaaaa cgtccggttc gtcgcccgcc
    20821 aaagcaatct gggacgccct cccgcggcat ggggtcaccc gcgggtggtg tatttgcagc
    20881 acgcctccga tccgatcgcc tggtggacgc ccgacctgct gttcaagagc ccggactggc
    20941 tcaatgaaaa gcggggctat gatgtgctgc cgcagacgca ttggatcccg ctggtgacgt
    21001 tcctgcaggt ctcagccgac atggcggtgg cggtggacgt gccggacggg cacggccacc
    21061 actacgtcgc cgacgtcgcc aacggctggg ccgacgtgct gtcgccgccc ggctggacgc
    21121 cggacaagac cgccaagctg cgaccgctgc tgcactccga cgccgtcatc agcggcccca
    21181 gcggctagct atatccagcg gcgttcgctc atcgcgagat cggcgtgcag cgcgtccggc
    21241 ggcgccgagc gccggcgcgc cgcggtgtgc agctgattca gcacgccggc gtccgccagc
    21301 gcctgatagc cgccgatggc gtcggtggcg cggtgcagac cgatgtccag cagtgcggcc
    21361 gccgccaggc tggaggtgta gccctgtgag cacacgatca cccactcgac gtcatcgccg
    21421 acggcctccg gcaccttggc ctcgctggtc gggtcgcagc gccattccag gacgttgcgt
    21481 tcgatcacca gcgcgcccgg caactcaccc tcgcggagtc gctggtcccg cggccggatg
    21541 tcgacaagga tcgcgccccg actcaccgcg tcgggcacct ccaccgcacg aaggcgccga
    21601 agccggcgcc gggctgctct caagacaagg tcgatgcggc tcattgggac ccctccggtt
    21661 gatcggtcag ttcggtgcgc tggcggcgca acctgttgcg gtcggtgacc tcgtagtacg
    21721 acatcgccgt cagcggcggc gagtaggcat gcacgctcaa ggtcggctgc accggcacct
    21781 tgaccgtgct cgccggccag gaaacgggcg tcgcaggcgt gggcactggc ctgggcgccc
    21841 acaccacgtc gtgcacccag cccagcggga aaccggcctg atcgccggcg tcgagccggc
    21901 ggcgccgcaa accccggccg tcccaacgga attcgttgag cgacccggac agcactgtca
    21961 gcgcccccag cgacccgccg tggtcatgca gttcggtggg gtggccgggc acccagctga
    22021 tcagccagat gtcgagttca tcgtcgccgt ggatgcgggt gaaccagcgc cgcgagtccg
    22081 gcacaccgcc cgcgggcagt agctggtcgc agcgtccgct gagcacatcg tcggcggccc
    22141 ggtcggtggc ctgcaggagg tctgggacac gcagccgggt gggtcccgat gacggggacg
    22201 acacggtcag cctgcggagg ggaagggggg cggcgacggg cgccgcaatg ggaagcgaca
    22261 ttgggagctc cagagagaat ggatcaggac gagggcggcg tgttaaggcc gacaacactc
    22321 gcaaaatccg aagcgctcca tcacggcagc aagtgttgca tagattggga acgtgcggtg
    22381 gagcagtcat cgccccttgc tgtggggcgc ggtcgtgctg gcgaccggtt tgtcggcggt
    22441 cacgggctgc tcggatggcg cgcccaccag cacgactggg tccccttcgg cgccgtcgtc
    22501 ggcgcggccg gccgacatcc ccaccggccc cagctcggca ccgccgagtg ccgtcgggct
    22561 ctcccccgcc ggcgtcacga ccaagatcga cgtccccgcg gattccaccg aagaggagta
    22621 ctaccaggcc tgtcacgccg cgaagctgtg gatggaagcg cagccaaaga ccggcgcgtc
    22681 gctcgtcgag ccctatctgg ccatgcttca ggcgtcaccg tcgggtaacg ccggcagctg
    22741 gaacgcccga tggtcggacc tgacgctggc gcgccaggcg gcggtgatca ccgccgc
//
LOCUS       DASSGQ010000112        32183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1101902,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000112 DASSGQ010000000
VERSION     DASSGQ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 32183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32183
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101902"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFI55_05255"
     CDS             complement(<1..309)
                     /locus_tag="VFI55_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 family protein"
                     /protein_id="HET7073475.1"
                     /translation="MASDVFDSEARLSWVLAVLAGVLGAIAFMHSAGYFVTFMTGNAQ
                     RSVLGYFRGDVRSSVTAAVLMLCFVAGVVVASVCRRRFWTGHPHGPTVLTTFSLLAAT
                     V"
     gene            complement(398..1759)
                     /locus_tag="VFI55_05260"
     CDS             complement(398..1759)
                     /locus_tag="VFI55_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltokinase"
                     /protein_id="HET7073476.1"
                     /translation="MTLPTKLPWSEWLPQQRWYAGRNRKLLATEVGLVVPLRDDLDLV
                     LVDAEYANGSSERYQVIVGWDSAPVSEYSTVATIGAAEGRTGFDALYDTDAPQFLLSL
                     IDSSAVRDSSGAEVKFAKEPGVKLPLEAMPHVSDAEQSNTSVIFDRRALFKVFRRVSG
                     GINPDIELNRVLGRAGNPHVARLLGTYELAVADADDSPDADETYPLGMLTEFASNAAE
                     GWAMATASVRDLFAEGDLYAHEVGGDFAGESNRLGEAVASVHATLAECLGTAQATFPV
                     DTVLSRLSSTVALVPELEEYATTIEERFQKLAGQTVTVQRVHGDLHLGQVLRTPESWL
                     LIDFEGEPGQPLDDRRAPDSPLRDVAGVLRSFEYAAYGQLVDQAADKQLAARAREWIK
                     RNCAAFCEGYAAVSGTDPRDSAEVLAAYELDKAVYEAGYEARHRPGWLPIPLRSIARL
                     TAA"
     gene            complement(1756..3576)
                     /gene="treS"
                     /locus_tag="VFI55_05265"
     CDS             complement(1756..3576)
                     /gene="treS"
                     /locus_tag="VFI55_05265"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HET7073477.1"
                     /translation="MNDTRDAVEHHPAQGSHVEGGVVEHPNAEDFGNAAALPADPTWF
                     KHAVFYEVLVRAFYDASADGAGDLRGLLERLDYLQWLGIDCIWLPPFYDSPLRDGGYD
                     IRDFYKVLPEFGTVDDFVALVDAAHRRGIRVITDLVMNHTSESHPWFQESRRDPDGPY
                     GDYYVWSDTSEKYSEARIIFVDTEESNWTFDPVRRQFYWHRFFSHQPDLNYENPAVQE
                     AMIDVMRFWLGLGIDGFRLDAVPYLFEREGTNCENLPETHAFLKRVRKVVDDEFPGRV
                     LLAEANQWPGDVVEYFGDPSTGGDECHMAFHFPLMPRIFMAVRRESRFPISEIMAQTP
                     PIPDMAQWGIFLRNHDELTLEMVSDDERDYMYSEYAKDPRMKANVGIRRRLAPLLEND
                     RNQIELFTALLLSLPGSPVLYYGDEIGMGDVIWLGDRDGVRTPMQWTPDRNAGFSTAN
                     PGRLYLPPSQDPVYGYQAVNVEAQRDTSTSLLNFTRMMLSVRRRHHAFAAGTFEELGG
                     SNPSVLAFIREASDGDTVLCVNNLSRFPQPIELNLQHWSGWTPVELTGHVEFPRVGHL
                     PYLLTLPGHGFYWFQLSVSDERMTRAGDDAKRSDGEERRP"
     gene            complement(3697..4776)
                     /locus_tag="VFI55_05270"
     CDS             complement(3697..4776)
                     /locus_tag="VFI55_05270"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HET7073478.1"
                     /translation="MGKWHHRTGRRSWLVGVLAVIGLGLGLTTVPASATTPGLSNLAL
                     DRFSDRPLTPLDPSAIVGQVGPQVVDISTKFGYNNAVGAGTGIVIDPGGVVLTNNHVI
                     SGATDISAWDVGNGQTYAVDVIGYDRAADVAVLQLRGGGGLPTANIGGGVAVGDPVVA
                     LGNAGGQGGTPSAVAGRVVALNQTVSATDTLTGADETLGGLIQADAAIRPGDSGGPMV
                     NSGGQVVGMNTAATDSYKMSGGQGFAIPIGTAMGVAGQIRSGAASNTVHIGPTAFLGL
                     GVMDNGGNGARVERVVGSGPAAAAGVSAGDVIIGVDNVPITGATSMSDVLVPHHPGDT
                     IFLHWRAKGGGDRSGPVTLADGPPA"
     gene            complement(4924..5388)
                     /locus_tag="VFI55_05275"
     CDS             complement(4924..5388)
                     /locus_tag="VFI55_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7073479.1"
                     /translation="MLAETQITNLLFRYAECIDDGDLAGAAALFEHARIRISGPDTGE
                     DTIDAARLLGIWKSLIVLHPDGTPRTKHVTTNPIIEIDEHAGTASCRSYYTVLQQTDD
                     LPLQTIVTGRYHDRFQRVDGQWRFSYRDLTLIDMVGDVSRHLRYPITPAARA"
     gene            5567..6013
                     /locus_tag="VFI55_05280"
     CDS             5567..6013
                     /locus_tag="VFI55_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HET7073480.1"
                     /translation="MGEFDPKVRFTQSPVARLATSTPDGVPHLVPVVFAVGGDQHDAH
                     AVVYTAIDAKPKTTQRLRRLTNIEKNPHASLLVDHYADDWTQLWWVRVDGVAAIHRDG
                     EVMRMAYRLLRAKYDQYQSVSLNGPVIAIAVRRWSSWHAAESVTRG"
     gene            complement(6014..6898)
                     /locus_tag="VFI55_05285"
     CDS             complement(6014..6898)
                     /locus_tag="VFI55_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019928409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HET7073481.1"
                     /translation="MITFYVLAGVAGLGALWLATTVRVVKQFERGVVYRFGRVQSRVR
                     GPGLALLIPVADRLEKVNMQIITMPVPAQDGITRDNVTVRVDAVIYFKVSDPVRAAVD
                     VQDYMSAIAQVAQTSLRSIIGKSNLDDLLCNREGLNQGLELMIDSPALGWGIHLDRVE
                     IKDVVLPDSMKRSMSRQAEAERERRARVITADGELQASRKLAQAAEVMAQHPAALELR
                     LLETVVEVAAEKNSTLVLPFPVELLRFLEGATQARSGMQGEPAAPSPESKPVANGANS
                     QWSAHRDVETFTAQEKFE"
     gene            7334..9490
                     /locus_tag="VFI55_05290"
     CDS             7334..9490
                     /locus_tag="VFI55_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-like protein EF-G2"
                     /protein_id="HET7073482.1"
                     /translation="MADKANASQGAGAAPTANGPADVRNVVLVGPSGGGKTTLVEALL
                     VAAGVLSRPGSVTDGSTICDYEDAEIRQQRSVGVAVASLSHAGTKINLIDTPGYADFV
                     GELRAGLRAADCALFVIAANEGVDEPTKSLWQECSQVGMPRAVVITKLDHARANYQEA
                     LAAAQEAFGDKVLPLYLPTGDGLIGLLSQSRYRYSGGKRTVHPPDPSDADRIEDERGT
                     LIEGIIEESEDESLMDRYLGGEAIDESVLIQDLERAVARGSFFPVIPVCSGTGVGTLE
                     LLEVATRGFPSPMEHPLPEVFTSHGAPHATLACDAGAPLLAEVVKTTSDPYVGRLSLV
                     RVFSGTIRPDTTVHVSGHFASFFGGAEGPNSKGNTHPDHDEDERIGVLSFPLGKQQRP
                     APSVVAGDICAIGKLSRAETGDTLSDKSEPLVLKPWTMPEPLLPVAIQAHAKTDEDKM
                     SVGLGRLAAEDPTLRIEQNQETHQIVLWCMGEAHVAVVLDALANRYGVTVDTVELRVP
                     LRETFSGKAKGHGRHVKQSGGHGQYGVCDIEVEPLPEGSGFEFVDKVVGGAVPRQFIP
                     SVEKGVRAQMERGVHAGYPVVDVRVTLLDGKAHSVDSSDFAFQAAGALALREAAAATK
                     VALLEPIDEISVLVPDDFVGAVMGDLSGRRGRVLGTDTAGHERTVVKAEVPQVELTRY
                     AIDLRSLSHGAASFTRAFVRYEPMPESAAARVKTSA"
     gene            9582..10052
                     /locus_tag="VFI55_05295"
     CDS             9582..10052
                     /locus_tag="VFI55_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007297801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HET7073483.1"
                     /translation="MSTFNGLPAHILLNHFVVVLGPLTAILAILCVLWPAARRRLIWL
                     VLLLAVGTTVLTPLTTSSGAWLASRVGTSPELATHEGLGATLIYMVAALLASVALLTA
                     VYLRQTRGRTVKLALHSVVGVLVILAALATLVQTYRIGESGARAAWGNVTSSAP"
     gene            complement(10111..11754)
                     /locus_tag="VFI55_05300"
     CDS             complement(10111..11754)
                     /locus_tag="VFI55_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadD7 family fatty acid--CoA ligase"
                     /protein_id="HET7073484.1"
                     /translation="MRSDSITGNDVVAERFAAQPDDETRGPRVADLVETAASVRPEAA
                     ALVVTSERIPVTYRDLTRLIDDLAGQLKRGGLLPGDRVALRAGSNAEFVVALLAASRA
                     DLIVVPLDPALPVAEQCIRSAAAGARVVLVDGNAPESAEATREPGLRRWPIAVSVVRE
                     AGAQQGTVAVNMDAGAPLEHDVSAPEGLRPDDAMIMFTGGTTGTPKMVPWTHHNIASS
                     VRAIVAGYGLGPQDATVAVMPLYHGHGLLAALLSTLASGGTVLLPAPGRFSAHSFWDD
                     IAAVRATWYTAVPTIHQILLERAKTERPGSSAALRFIRSCSAPLTPETAQALHDTFAA
                     PVVCAFGMTEATHQVATTRIESVDQDESPAATTGLVGRSTGPEIRIAAPDGQPLPAEA
                     VGEVWLHGPTVVRGYLGDPAITAANFTDGWLRTGDLGTLSTAGDLSIRGRIKELINRG
                     GEKISPERVEGVLASHPNVLEVAVFGVPDKLYGETVAAVIVPRKSPSPSAAELAVFCR
                     ERLAAFEVPSAFREAGALPHTAKGSLDRRAVADRFGGRR"
     gene            12017..13765
                     /gene="oxc"
                     /locus_tag="VFI55_05305"
     CDS             12017..13765
                     /gene="oxc"
                     /locus_tag="VFI55_05305"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HET7073485.1"
                     /translation="MATVSASPSTAPESTRLTDGFHLVVDALKANDIDTIYGVVGIPI
                     TDLARIAQASGIRYIGFRQETSAGNAAAAAGFLTGRPGVCLTVSAPGFLNGLAALANA
                     TTNCFPMIQISGSSDRAMVDLQRGEYGELDQLSAAKPFVKAAYRIGKVEDIGRGVARA
                     IRAAVSGRPGGVYLDIPGDVLGQAMAASAASGTIWRVVDPAPRQLPAPEAVDRALDVL
                     AQARRPLIVLGKGAAYAQADSVIREFVEATGIPFLPMSMAKGLLPDSHPQSAAAARSL
                     AVARADAVLLVGARLNWLLGHGESPQWSADAKFVQVDIAASEFDSNQPIAAPLVGDIG
                     SVMSALRDGLAAHPIAVPAEWTSELADRKARNDAKMRQRLAEDPHPMRFYNALGAIRS
                     VLQDNPGVYVVNEGANALDLARNVIDMELPRHRLDTGTWGVMGIGMGYAIAAAVETGK
                     PVVAIEGDSAFGFSGMEIETICRYRLPVTVVILNNGGVYRGDEASPGGDPAPTVLNAR
                     ARHELIAEAFGGKGYHVTTPAQLGSALAEAIASNGPSIIDCELDPAAGVESGHLASLN
                     PTSAATPPPAAVNVGA"
     gene            complement(13749..14681)
                     /locus_tag="VFI55_05310"
     CDS             complement(13749..14681)
                     /locus_tag="VFI55_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7073486.1"
                     /translation="MLFRQLEYFVAVAQERHFARAAEKCYVSQPALSAAIAKLERELN
                     VTLINRGHSFEGLTPEGERLVVWAKRILAEHDSFKAEVHAVRSGITGTLRLGTVPTAS
                     TTASLVLSAFCSAHPLAKVQILSRLAATELYRRLREFDLDAVIVHTAPDDGHEVSLVP
                     LYQEHYVLLSSTDMLPSGASTLTWPEAAQLPLALLSAGMRDRQIIDEAFAAHGITVAP
                     QVETDSVASLLAQVSTGNWACIVPHTWLWTSPLGAGIRAVEMVDPVVQAEIALATNSG
                     GPGSPIARALAACAQELALNEFFDAQLLGITRRR"
     gene            15208..15963
                     /locus_tag="VFI55_05315"
     CDS             15208..15963
                     /locus_tag="VFI55_05315"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET7073487.1"
                     /translation="MRRAVRYLFVMVAITAMGALGSGTRSVAAVPVPAPVPGVASVLP
                     ADGALVGVAHPVVVTFTTPVSDRGTAERSIHIASPSNTQGHFEWAESNVVQWVPNHYW
                     PSHTHVSVGVQALTTGFDTGDELLGVASLSAHTFTVSRDGEVLRTMPASMGKPTRPTP
                     IGDFTALEKDRTVVMDSRTIGIPLSSPEGYKITAQYAVRVTWGGVFVHSAPWSVDSQG
                     HANVSHGCINLSPDNAAWYFNQVNVGDPIEVVA"
     gene            complement(15998..16612)
                     /locus_tag="VFI55_05320"
     CDS             complement(15998..16612)
                     /locus_tag="VFI55_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073488.1"
                     /translation="MELARITVAGWAVATAVIATATAGCGNDHNASSPSSSSSATSST
                     SSSTVTSPAPGQPSDYSSLLIKASDIGGDLTTPQPPVLNPNNAAGVAQLFANADNSRR
                     IGDTILIVADPAAAVAGLENTKTNYAGRVTGTWQPVNVGSNGAMISGTSPDNSQAVTV
                     LLFTEGKALVNLEFDSAPNDPIDAAVATDVGRKQDAAIKSGLPS"
     gene            16812..17762
                     /locus_tag="VFI55_05325"
     CDS             16812..17762
                     /locus_tag="VFI55_05325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HET7073489.1"
                     /translation="MTAGARAVRFDRYGGRDVLYVTDIDMPSPGLGEVVVEVRAAGIN
                     PGEAAIRTGALRDIFPATFPSGEGSDLAGVVTAIGPGVTEFSVGDEVLGFSFRRSSHA
                     THTAVPVDQLVRKPPQLSWEVAGSLYVVAATAYAAVRSVSPQPGDTVAVSAAAGGVGS
                     LVVQLLALHKARVLGIAGPGNADWLRAHGVIPIAYGNDLQELATRLREAAPEGIDAFI
                     DLFGPDYVQLAVDLGIAPERIDTIIAFQKAVEVGAKTEGSAEASTPEVLAEMADLVAT
                     GALDFDVAATYPLDRVADAFEELERRHTHGKIVLLPNGSQ"
     gene            complement(17866..18081)
                     /locus_tag="VFI55_05330"
     CDS             complement(17866..18081)
                     /locus_tag="VFI55_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073490.1"
                     /translation="MAFVVTHRVSGLTRGSGRSLLHSGRLLAGKLHARRRLTPQERAN
                     LYVSRMPIAVITASLGGHPSDSAGGRR"
     regulatory      complement(18357..18531)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            18679..19338
                     /locus_tag="VFI55_05335"
     CDS             18679..19338
                     /locus_tag="VFI55_05335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011742312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7073491.1"
                     /translation="MPTTLQDPKVAAVLDRMYAESQAQMSLLRERRGQFERPMTTAER
                     TEAMSEFYIPVTPEAGRLLYALVRATRPTTVVEFGMSFGISAVHLASAVRDNGTGRVV
                     TTELSDTKIAAAKKTFAETGLDDVITILEGDALSTLASLDGPVDFVLLDGWKDLYLPV
                     MKLLEPRLPTGALIVADNTSAADTGPYLDLVRNPDNGYTSFNFLVRDSDSMEISCRTD
                     D"
     gene            complement(19342..21324)
                     /locus_tag="VFI55_05340"
     CDS             complement(19342..21324)
                     /locus_tag="VFI55_05340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003917883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HET7073492.1"
                     /translation="MAIWDKPERRSGIPALDGLRAVAVALVLAGHGGIPGVGGGFIGV
                     DVFFVLSGFLITSLLLDELGRTGRIELTGFWIRRARRLLPALVLMVLTVGAARELLPT
                     QALTGLRNDAIAAFLWVANWRFVAQKTDYFTQGAPPSPLQHTWSLGVEEQYYFVWPVL
                     LIAVAVLLAARARRYFMRATVGGVRFAVFAIATLGALASAAAAIVFVSGGTRDRIYFG
                     TDTRAQALLIGSAAAALLVRDWPSLNRGWCMIRTRWGRRVARLLPFVGVAGLAGATHY
                     ATGSVGEFRHGLLTGVAIASVAVVASVAVEQRGAIARVLAVPPLVWLGTISYGVYLWH
                     WPIFLALNGERTGWTGLPLFGARCAATVVVAAASWWLIEQPIRRWRPARVPLLPLAAA
                     TVASAAAVTLLVIPVGTGPGLREVGLPPGVSAVAAVSPSPPGESRARPRDPNRPFTVS
                     VFGDSIGWTWMHYLPPTPGFTFLDHTVIGCSLVRGTPYRYIGQTLEQRAECDGWPIRW
                     STQVNQDRPDVALLIIGRWETVDRVNEGQWTHIGDPTFDAYLNGELERALNIVSSTGI
                     RVVVATVPYSRGGEKADGRLYPEDQPDRVNLWNTMLRNTVSHHPNVAILDLNKKLCPD
                     GVYTAKVDGIKVRSDGVHLTPEGVKWLTPWLEESLR"
     gene            complement(21580..22788)
                     /locus_tag="VFI55_05345"
     CDS             complement(21580..22788)
                     /locus_tag="VFI55_05345"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET7073493.1"
                     /translation="MPSRLPNSSNRGGPLAGVRVVDLTAMVMGPYCTQIMADMGADVI
                     KVEPPKGDDTRYVSVGPVRGMGGVFVNVNRGKRGVTLDLQSDWGKSVLRALIEQADVF
                     IHSMRSKAVARLGFSYDEVAAINPGIVYTNCYGYSRRGPNGDLPAYDDTIQAACGLPF
                     VQEQLTGEPNYVGTILADKVAGLTALYATMMALFHRERTGEGQEVEVGMFETLASFML
                     VEHANGAMFDPPLGPAVYPRTVAPNRRPYRTSDGYVAALIYNDKHWAAFIDAVKPPWA
                     SDLYATLERRARQIDTVYALLAETLAQRTTDEWIELFRRLEIPAASLSSPANLFDDEH
                     LNAVGFFETVDTPHGPVRFPGVPTWFSRTPGHVAGPAPELGADTAEVLEELGLGLADV
                     RGDVKPAGQA"
     gene            complement(22781..23713)
                     /locus_tag="VFI55_05350"
     CDS             complement(22781..23713)
                     /locus_tag="VFI55_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7073494.1"
                     /translation="MTVERRFDNRVAVVTGAGRGLGREYALLLASRGAKVVVNDNGGA
                     LTGAGTDAGPAQQVVAEIRAAGGDAVACVESVATAEGGQAIIGAAQDRYGRIDILIHN
                     AGNVRRGSLKEMSYQDFDAVIDVHLRGAFHVLRPAFPIMCDAHYGRIVLTSSIGGLYG
                     NHGVANYAAAKAGLVGLANVVALEGAADGVLCNVIVPSAVTRMAEGLDISAYPPMGTE
                     LVAPVVGLLAHETCPITGEMLIAIAGRVARVVVAETPGVQRPSWSIEDVAEQLDAIRC
                     AAAPLVFPVVPDGHADHIRYSFETALRKEGVRHA"
     gene            complement(23710..25572)
                     /locus_tag="VFI55_05355"
     CDS             complement(23710..25572)
                     /locus_tag="VFI55_05355"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7073495.1"
                     /translation="MTDISRPDCPPTQTPDDVDIGALREKYRGERDKRLRPEGSKQYV
                     ELVDDFAGYYETDPYSPPLVRDPISEDIDVAVLGGGFGGLLCAAYLKKAGVDDVRIIE
                     LGGDFGGVWYWNRYPGIQCDNESYCYIPLLEELNFIPSKKFADGAEIYDHCRRIGKHY
                     GLYDSAIFSTQVRELHWDEAIKRWRVGTDRDDDIRARFVVMASGPFHRPKLPGIAGIM
                     DFQGHSFHSSRWDYGYTGGDSSGGLDKLADKRVAVVGTGATAIQIVPYLGRYARHLYV
                     FQRTPSSVGARNNTPTDPQWVKTLRPGWQKERQRNFHAWTFEGMALGQPDYVCDFWTE
                     LGRNTAARVMALEDPAALTPEQFMAIREEEDFKVMERLRRRVDAIVGDPGTADALKPY
                     YRFLCKRPCTNDDYLHTFNRPNVTLVDVSSTKGVEGATAKGLVANGIEYEVDCIIYAS
                     GFEITTEISRRYSIDAIEGRDGLSLFDHWRSGYQTLHGMTSRGFPNQFFTGFTQVGIS
                     ANIAANYELQGEHIAYIISEALTRGALTVEPTQEAQDEWCKAIRENAIDNSRFDLECT
                     PGYYNNEGGGAGVAEGEGIRSHIGEPYGPGFYVFGELLSEWRAQGDLDGLVLGS"
     gene            complement(25569..26744)
                     /locus_tag="VFI55_05360"
     CDS             complement(25569..26744)
                     /locus_tag="VFI55_05360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011742317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7073496.1"
                     /translation="MKVNLGFGSHNSHDWERVLAGDFSHPPVTADWECVQATLAIADL
                     AEPLGFDGIWMPEHCGTPYGMTPNPIQALSYFAGRTERVSLGTFVVVAPWWHPIRLAH
                     QIAYLDILSNGRYTTIGIGRGVSKGEFDAVGVPREESRQRFNETLDILQLALSGERFS
                     YEGEIFTVPEMSLRPEPRTRDLFSRIYSSSSTAESLEILARRGMVPLFVGNKPIEDAG
                     REVQQVNIFRKEEGLPPCQPKNVMFMYCTTEDPARSEEWIWTANRDVTVHYGFADASN
                     FKGVKGYEAYAAREASATAVLASSVTGAAKGGQPKTPGYHASNLLIGTPEEIFTRIKA
                     AQEACSFCELTIVPQFGTMPYPEAMASTELFAKEVLPDVQKMEAPLHAAALPENALA"
     gene            complement(26741..28114)
                     /locus_tag="VFI55_05365"
     CDS             complement(26741..28114)
                     /locus_tag="VFI55_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET7073497.1"
                     /translation="MDRADELATPVTIPVEAYISEEYAHAERDKLWRKVWQQVGRIED
                     LPKVGSYLTYDILDDSVVVVRTGPDSLAAHHNVCMHRGRRLVDVPAGAKSACGHKKSF
                     VCGFHGWTYDQDGNCIHVPEKQDWQGALTPENTHLATVNVDTWGGWIWVNMDPGCEPL
                     RDYLEPAATMLDPFQLQDMRCKWRKWLHFGCNWKVAMEAFNETYHVATTHPQFTKFGN
                     FRGWAVAHGKHSNIGYDAPADLEETKSKIRLGTGADPRISTAEMQVYTLDETNATTTK
                     TLVEAACRLVDELPEGAPAGQVLEHWLASARRDDAARGVTWPTIDPEHLARSGTAWQI
                     FPNFQIGQGLTTALCYSARPHGYDPDSCIFEAACYELYPKGEEPQTEWVYAPPGDPNW
                     RSVLPQDFSNMAAVQQGMKSLGFSGPKPNPYMERSTANLHRNLAEYLGTGAPSKLATD
                     LKRETGE"
     gene            complement(28145..29023)
                     /locus_tag="VFI55_05370"
     CDS             complement(28145..29023)
                     /locus_tag="VFI55_05370"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020731394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7073498.1"
                     /translation="MNRDLLRLDGRIVVVSGAAGGGIGTTVTRMVAEAGATVVAVSRD
                     QDNLDRHVKPLLAEGLSVVPLAADASTDDGVDATLEAVRRTEGELHGLVNVAGGADPS
                     TWMPSTRVTRSDWRELFTRNLETMFFMSQAVAAELKRRKCGGSIVSVSSISGMNTAPF
                     HIAYGTAKAAIVAVTRTMALELAAGDEGSRGTVIRVNAVAPGVTRTPASGTYVDDDPE
                     RDRHAIAMGRRGCPEEVAGAILFLLSDLSSYITGQTLLVDGGLNLKWGHLGADNTSLF
                     LKDETFRAAITRWDGA"
     gene            29188..30840
                     /locus_tag="VFI55_05375"
     CDS             29188..30840
                     /locus_tag="VFI55_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconolaconase"
                     /protein_id="HET7073499.1"
                     /translation="MAPHGLSQGRYLAARQPSLAEGWSLTRLTPPSRLFGANGLRTGP
                     DGRVYIAQVTGSQISALDVTTGRLDVISAKGGEIVAPDDVAFDPSGNLYATEVMDGRV
                     SLRDAAGRTRVLRADVPSANGITFHRGRLFIGECRAGGRLLELDLNGGAPRVLLENAP
                     SPNAMEVGPDGLLYFPLMGANEIWRIDPDGGEPERVAGDLGVPDAVKFDSSGRIVSTQ
                     VHSGQVLRIDPRTGDRTVLAALNPGLDNLTFVGERLFVSNFTGEIAEILGGGQARTAL
                     PGGMNWPLDLAVDTNGDLYVADGTYFYALGAGGRSGSSPIRPPTLRTVGMLFSPGYPG
                     FLRGLAATGAGEFVVTTSNGQLARYRPGSNESEVLAEGFDQLYGVAVAPGGAIVAAEL
                     GAGRVLAIRSGLVEVLAAGLNDPIGVAIAPDGACFVAEAGAGRVVRIGGTGARITTAL
                     DGLRRPHGILMLDGRLYVVDVGAQELIEFDLASGDRGVVASDLPVGAPPGVVPKPLLG
                     MPPFSGPQGPFAGIAAGPDGTLYVSADAEGSVLAIHREGAED"
     gene            30842..31591
                     /locus_tag="VFI55_05380"
     CDS             30842..31591
                     /locus_tag="VFI55_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7073500.1"
                     /translation="MSEPKVVDHRYLQVARTLRKEIVDGVYPVGSQLPTEHELCERFA
                     VSRYTIREALRRLREDNLVSSRPRTGTLVVPRPSTDSYVQHVMSINDLLAFATGTHFP
                     IESVAMVRLDDELAARTGLTLGEQWLSVRGFRRAEGAQSAEPALCRTEYYINRAFAAV
                     GRLLARHEGPIFPLIEDLFGLSIVEVHQEIAAVLIPPALADGLDVEAGAPALQVRRTY
                     TTSDGQIAQVTINTHPASRFRHSMTMRRMKG"
     gene            31617..>32183
                     /locus_tag="VFI55_05385"
     CDS             31617..>32183
                     /locus_tag="VFI55_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7073501.1"
                     /translation="MPRHRERKLTGGRSIRWDDERAADAYARGWWVRGTLADSLRDAA
                     RRTPQRVALVDGDRWVDCQSLCRQATTLANALHARIPPGSVVSFMLPNWHEAAVIYLG
                     ATMAGMVVNPILPSLRDRELAFILSDCDSRAIFVPSSFGKHDYVAMLDRVTTDMTSPP
                     EVVVLRGGDERSLPLEKSSKFRAVRNVRG"
BASE COUNT         5466 a        10735 c        10514 g         5468 t
ORIGIN      
        1 caccgtggcc gccaacaaac tgaaagtcgt cagcacggtc ggaccgtgcg gatgccctgt
       61 ccagaagcgt cgccgacaca ccgaggcgac caccacgccg gcgacgaagc acagcatgag
      121 caccgccgcc gtgaccgacg accgtacatc gccccggaag taccccaaca ccgagcgctg
      181 ggcattaccg gtcatgaagg tgacgaagta gcccgcggag tgcataaacg cgatcgctcc
      241 gagcacaccg gccagcaccg ccagcaccca cgacaaccga gcttcgctat cgaatacgtc
      301 actggccacg cggacatgct ttcagacaac ccgcggcggg cgcagcgcgg acgagctggg
      361 tcccctttgc cccgcgagcg cggacagaat gcccgcgcta ggcagcggtg agccgggcga
      421 tggaccgcaa cgggatcggc agccatccgg gccggtgtcg ggcctcatag ccggcctcgt
      481 agacggcttt gtcgagttcg taggcggcca gcacctccgc cgagtcccgg gggtcggtcc
      541 cggacacggc cgcgtagccc tcgcagaacg cggcgcaatt gcgcttgatc cattcccggg
      601 cgcgggcggc caactgcttg tcggcggcct ggtccaccag ctgtccgtag gccgcgtact
      661 cgaaggaccg caacacaccg gccacgtcgc gcaacggcga atccggcgcg cgccggtcgt
      721 cgagcggctg gcccggctct ccttcgaagt cgatcagcag ccagctctcc ggcgtgcgca
      781 gcacttgacc aaggtgcaga tcaccatgca cccgctggac ggtgaccgtc tggcccgcga
      841 gcttctggaa ccgctcctcg atcgtcgtcg cgtactcctc cagctcggga accaaagcca
      901 ccgtcgacga cagccgcgac agcacggtgt ctaccgggaa cgttgcctgc gccgtgccga
      961 ggcattcggc cagcgtggcg tgtacagagg caacagcctc accgagccgg ttggactcgc
     1021 cggcgaaatc cccgccgact tcgtgggcat agaggtcgcc ctccgcgaac aggtcacgaa
     1081 cgctggcggt ggccattgcc cagccctcag cggcgttgga cgcaaattcg gtcagcatcc
     1141 ccaggggata tgtctcgtca gcgtccggcg aatcatcggc gtccgctacc gcgagctcgt
     1201 aggtgccgag tagacgggcc acatgcgggt taccggcgcg ccccagcacc cggttcagtt
     1261 caatgtcggg gttgatgccg ccgctgaccc gacggaacac cttgaaaagg gcgcgtcggt
     1321 caaagatcac actggtgttg ctctgctcgg cgtccgaaac gtgcggcatc gcctccagcg
     1381 gcaacttaac gcccggttct ttggcaaatt tcacttcagc acccgacgaa tcgcggacgg
     1441 ctgacgagtc gatcaaggac agcaggaact gcggtgcatc ggtgtcatac agggcgtcaa
     1501 agccggtccg gccctcggcg gcgccgatcg tggccacggt gctgtactcg gagaccggcg
     1561 cggaatccca cccgacgatc acctggtagc gctccgacga accgttggca tattcggcgt
     1621 cgaccagcac caggtcgagg tcgtcgcgca gcgggacgac caatccgacc tctgtggcca
     1681 acagcttgcg gttgcgtccg gcataccaac gttgttgcgg cagccactca gaccaaggca
     1741 gcttggttgg gagagtcatg ggcgccgctc ctccccatcg cttcgctttg catcgtcgcc
     1801 ggcgcgggtc atgcgttcgt cagataccga caactggaac caatagaagc cgtgtcccgg
     1861 cagtgtcagc aggtagggca ggtggccgac gcgtggaaac tccacatgcc cggtcaactc
     1921 caccggcgtc cagccgctcc agtgctgcag gttcagttcg atgggctgag ggaatcgcga
     1981 caggttgtta acgcacagca cggtgtcgcc gtctgaagcc tcgcggatga aggccagcac
     2041 cgacgggttc gaacccccca attcttcaaa cgtgccggcc gcaaaggcat ggtgtcggcg
     2101 gcgcaccgag agcatcatgc gggtgaagtt gagcagcgac gtcgaggtgt cgcgctgggc
     2161 ctccacgttg actgcctggt agccgtagac ggggtcctgg ctcggtggca ggtatagccg
     2221 accggggttg gcggtggaaa acccggcatt gcggtccggc gtccactgca tcggcgtccg
     2281 caccccgtcg cgatcgccca gccagatcac gtcgcccata ccgatctcat cgccgtagta
     2341 gagcaccggc gagccgggca gcgacagcag caacgccgtg aacagctcga tctggtttcg
     2401 gtcgttttcc agcaacggcg ccagccgtcg acggatcccg acgttcgcct tcattcgcgg
     2461 gtccttggcg tactcggaat acatgtagtc gcgctcgtcg tcgctgacca tttccagggt
     2521 caactcgtcg tggttgcgca aaaagatccc ccactgcgcc atgtcgggaa tcggcggcgt
     2581 ctgcgccatg atctccgaga tcggaaaccg cgactcgcga cggaccgcca tgaagatccg
     2641 cggcatcagg gggaagtgaa acgccatgtg gcattcgtcg cccccggtac tggggtcgcc
     2701 gaagtactcg accacatcgc cgggccactg attggcctca gccaacagca cgcggcccgg
     2761 gaactcgtcg tcgacgactt tgcggacgcg cttgaggaac gcgtgcgtct cgggcaggtt
     2821 ctcgcagttg gtgccctcgc gttcgaacag gtagggcacc gcgtcgagcc gaaacccgtc
     2881 gatcccgagc ccgagccaga agcgcatgac gtcgatcatc gcctcctgca cggccgggtt
     2941 ttcatagttc aggtccggct gatgagagaa gaaccggtgc cagtagaact ggcggcggac
     3001 tggatcgaat gtccagttcg actcttcggt gtcgacgaag atgatccggg cctcgctgta
     3061 cttctcgctg gtgtcgctcc acacgtagta atcgccatac gggccgtcgg gatcgcggcg
     3121 tgattcctgg aaccacgggt gcgactccga cgtgtgattc atcaccaggt cggtgatgac
     3181 ccggatgccc cgccggtggg ccgcgtcgac cagcgcgacg aaatcgtcga cggtgccgaa
     3241 ttcgggcaag accttataga agtcccggat gtcataacca ccgtcgcgca gcggcgaatc
     3301 gtagaacggg ggcagccaga tgcaatcgat gccgagccac tgcaggtagt cgaggcgctc
     3361 gagcaggcca cgcaggtctc cggcgccatc cgcgctcgcg tcgtagaacg cccgaacaag
     3421 tacctcgtag aacacggcgt gcttgaacca cgtcggatcg gcgggcaatg cggcggcgtt
     3481 gccgaaatcc tcggcgttcg ggtgctcgac cacacccccc tcgacgtgac tgccctgcgc
     3541 gggatggtgc tcgacagcgt ctcttgtgtc gttcatcaat tccacgctgc cacgggtacc
     3601 cgaggcggga caccgggaat cacgactgcg gaaagccaac gaatcacgcc ggaccgcaaa
     3661 cgaattgagc gagggcgggc atcatccgcg ccgatatcag gccggcggtc catccgccaa
     3721 ggtcacgggt ccgctacgat cgccgccccc cttggcgcgc cagtgcagga aaatcgtgtc
     3781 cccggggtga tgcgggacga gcacatctga catcgaagtc gccccggtga taggcacatt
     3841 gtcgaccccg atgatgacgt cgccggccga aaccccggcc gccgccgccg gaccgctgcc
     3901 gaccacgcgc tcgacccgcg cgccgttgcc gccgttgtcc atgacgccaa ggccaaggaa
     3961 ggccgtcggg ccgatgtgca ccgtgttcga cgcggcacca ctccggatct gcccggccac
     4021 tcccatcgcc gtaccgatcg gaatggcgaa tccctgtccg ccggacatct tgtagctgtc
     4081 ggtggcggcg gtattcatgc cgaccacctg gccgccgctg ttgaccatgg ggccgcccga
     4141 gtcgccgggc ctgatcgcgg cgtcggcctg aatcaacccg cccagcgtct cgtcggcgcc
     4201 ggtcagggtg tcagttgccg agacggtctg gttgagcgcg acgacacggc cggccaccgc
     4261 actgggtgtt ccaccctggc caccggcgtt gcccagcgcg accacggggt cgccgacggc
     4321 gaccccgcca ccgatgttgg cggtgggcag gccacccccg ccacgaagct gcaggacggc
     4381 cacgtccgcg gcccggtcat agccgatcac gtccacggcg taggtctggc cattgccgac
     4441 gtcccacgcg ctgatgtcgg tggcgcccga gatcacgtgg ttgttggtca acacgacacc
     4501 acccgggtcg atgacaatgc ccgttccggc acccaccgcg ttgttgtaac cgaacttggt
     4561 gctgatatcg accacctgcg gcccgacctg cccgacgatc gcggaggggt cgagcggcgt
     4621 caggggccgg tcgctgaacc ggtcaagcgc caggttggac agcccaggag tggtggcgga
     4681 cgccggcacc gtggtgaggc ccaggcccag tccgatcaca gccagcacgc cgactaacca
     4741 tgaacgccgg cctgtccgat ggtgccattt gcccatcccg tcacctccaa gatcgcatta
     4801 ggaaccagaa ctggcccagc ctccgggtct gggcggcacg acataattcg tataaccgta
     4861 atgcaggtag ctatgtagga cagtgttcag gccctcgttg gccgcgcgcc cgcgaatcct
     4921 tgctcatgcc cgcgcggctg gcgtgatggg gtatctcagg tggcggctga cgtcaccgac
     4981 catgtcgatg agtgtgaggt cgcggtagga aaaccgccac tggccgtcga cgcgctggaa
     5041 tcggtcgtgg tagcggccgg tgacgatggt ctgcaggggc aagtcgtctg tctgctgcag
     5101 caccgtgtag taactgcggc agctcgcggt gccggcgtgc tcatcgatct cgatgatcgg
     5161 attggtggtc acatgcttgg ttcgcggtgt cccgtccgga tgaagcacga tcagcgattt
     5221 ccagatgccc agtagccggg ccgcgtcgat cgtgtcctcc ccggtgtctg ggccgctgat
     5281 ccggatgcgc gcgtgttcga acagcgccgc cgctcccgcc aaatccccgt cgtcgatgca
     5341 ttcggcgtag cggaaaagga ggttggtgat ctgcgtctcc gccagcatcc ggttaaccta
     5401 gcgcggcgac gctaaacttc cgagccgacc ggaacggtcg gaggtggggg tacctcccag
     5461 ccgcgaagcg gcgaggggga aagccgggca actcagcctc agcgcggcgg cccaaacttc
     5521 caagcggccc ctggggccgg ccgcggtcgg ctcctaagct gacacggtgg gcgaattcga
     5581 cccgaaggtc aggttcacac agtccccggt ggcgcgactg gccaccagta cgcccgacgg
     5641 agtgccacac ctggtgccgg tggtattcgc agtaggcggc gaccaacacg acgcccatgc
     5701 ggtggtgtac accgccatcg acgccaaacc gaaaacgacg caacggctgc gccgcctcac
     5761 caatatcgag aaaaaccccc acgccagcct gctcgtcgac cactacgccg acgattggac
     5821 ccaattgtgg tgggtccggg tcgacggtgt ggccgcaatt catcgtgacg gcgaggtgat
     5881 gcgcatggcg taccggctgc tgcgcgccaa atacgaccag taccaatcag tttcgttgaa
     5941 cggtccagtc atcgcgattg cggtgcgccg ctggtccagc tggcacgccg ccgaatcggt
     6001 gacacgcggg tgactactcg aacttctcct gcgcggtaaa agtttcgacg tcacggtgtg
     6061 ccgaccactg cgagttcgcc ccgttggcaa cgggtttcga ttccggcgac ggagcggccg
     6121 gctcaccttg catgccggaa cgtgcctgtg tcgcgccttc caggaacctg agcagttcga
     6181 ccgggaacgg caacaccagc gtggaatttt tctcggcggc aacctcgacc accgtttcca
     6241 acagccgcag ctccaacgcg gccgggtgct gcgccatgac ctccgccgcc tgggccagtt
     6301 tccgcgacgc ctgcaactcg ccgtcggcgg tgatcaccct ggctcgccgc tcccgctcgg
     6361 cctcggcctg gcgcgacatc gacctcttca tcgaatcggg cagcaccacg tctttgattt
     6421 cgacgcggtc gaggtgaatt ccccagccca gcgccgggct gtcgatcatc agctccaggc
     6481 cttggttcag gccttcgcgg ttacacagca gatcgtcgag attgctcttc ccgatgatcg
     6541 accgcagcga ggtctgcgcc acctgcgcga tcgcggacat gtagtcctgc acatcaacag
     6601 cggcccgcac cggatccgag accttgaaat agatgaccgc gtctacgcgc acggtgacgt
     6661 tgtcgcgggt aatgccgtcc tgggcgggta ccggcatggt gatgatctgc atgttcacct
     6721 tttccagccg atcggcgaca ggaatcaaca acgcgagccc gggcccgcga acacgggact
     6781 gcacgcgccc gaaccgatac accacacccc gctcgaattg cttcacgact cgaacggtgg
     6841 ttgccagcca cagcgcgccg aggccggcca ctccggccaa cacataaaag gtgatcattg
     6901 ccgtctccat ttccgcccac acaattccgc gtcgctttcc gcatgcgggc cgacgctggg
     6961 gcacctgggg acagccgtaa tcaaggcggt cacccactgt gaggtccgcc gacctccacc
     7021 gaaccagact ctgacccgga ggccggagtt catgtatccg attctggcac tagcgcgaag
     7081 tttcggcacc gatgttttgt caggttcggg tcgatgacga aagaccgtgc cctgcgagcc
     7141 gactgcgggc gcccggcggt cgcccgagct cgaccgttct ctgctcgccg cacagaagtg
     7201 gccttgtgtt gggcggcagt tgggggaaaa gttgcctaca gcggcctgtg gtccaggaca
     7261 catgacgcca tagcacccag cgggtgacca caggcgccgc gagcgaaggc gacctgcaga
     7321 actggaggaa ctcatggcgg acaaggcgaa cgcttcccag ggtgcgggag ccgctcccac
     7381 cgcgaatggt ccggcagatg ttcgcaacgt cgttctggtt ggaccctcgg ggggcggcaa
     7441 gaccacgctg gtcgaggcct tgctggtcgc cgcgggcgtg ctttccaggc cgggttccgt
     7501 caccgacggc agcacgatct gcgactacga agacgccgag atccggcaac agcggtccgt
     7561 gggcgtcgcc gtcgcctctc tgtcacacgc gggcaccaag atcaatctga tcgacacacc
     7621 cgggtacgcc gacttcgtgg gcgagttgcg ggccggtctg cgagccgcgg attgcgcgct
     7681 gttcgtgatt gcggcaaacg agggtgtcga cgagcccacc aagtcgctct ggcaggaatg
     7741 cagccaggtc gggatgcccc gcgccgtggt gatcaccaaa ctcgaccacg cccgggcgaa
     7801 ctaccaggaa gcgctggccg ccgcgcagga ggcattcggc gacaaggttt taccgcttta
     7861 cctgccgacc ggtgacggcc tgatcggatt gctgtcgcag tcacgctacc ggtactcagg
     7921 gggcaagcgg accgtgcatc cgccggaccc gtccgacgcc gatcggatcg aagacgagcg
     7981 cggcaccctt atcgaaggaa tcatcgagga atccgaggac gagtccttga tggatcgcta
     8041 tctcggtggc gaagctatcg acgagtcggt actcatccag gacctcgaga gagccgttgc
     8101 ccggggttcg ttcttcccgg tgatcccggt gtgcagcggc accggtgtcg gcaccctgga
     8161 attgctggag gttgccactc gcggattccc gtccccgatg gaacacccac tgccggaggt
     8221 cttcacgtcg cacggtgctc cgcacgcaac gctggcctgc gacgccggtg cgccgctgct
     8281 cgcggaggtg gtgaagacga cgtcggaccc ctacgtcggc aggctcagcc tggtccgggt
     8341 gttctccggg accatcaggc ccgacacgac ggttcatgtg tcgggccatt ttgcgtcgtt
     8401 tttcggcggg gccgagggcc cgaactccaa gggcaacacg catcccgacc acgacgaaga
     8461 cgaacgcatc ggggtcctgt ccttcccgct cggcaagcag cagcgccccg cgccgtcggt
     8521 ggtcgccggc gatatctgcg cgatcggcaa gctgagccgg gccgaaaccg gcgacacact
     8581 ctcggacaag tccgagccgt tggtcctcaa accctggacc atgcccgagc cgctgctgcc
     8641 ggtggccatt caggcgcacg ccaagaccga cgaagacaag atgtcggtcg gtctggggcg
     8701 gctggcggcc gaggacccga cgctgcggat tgagcagaat caggaaacgc accagatcgt
     8761 gttgtggtgc atgggcgagg ctcatgtcgc cgtcgtcctc gacgcgctgg ccaaccgcta
     8821 cggcgtcacc gtggacacgg tggagctgcg cgtcccgctg cgagaaacgt tcagcggcaa
     8881 agcgaaaggc cacggccgcc atgtcaagca atccggcggg catggtcaat acggggtctg
     8941 cgacatcgag gtggaacccc tgccggaagg ctccggattc gagttcgtcg acaaggtggt
     9001 cggcggagcg gtgccgcgcc agttcatccc cagtgtggag aagggggtgc gcgcgcaaat
     9061 ggaaaggggc gtgcacgccg gttacccggt cgtcgatgtc cgggtcacgc tgctggacgg
     9121 caaggcacac agcgtggatt cgtcggattt cgcctttcag gcggccggcg cactcgcgtt
     9181 gcgggaggcg gccgcggcga cgaaggtggc gctgctcgag ccgatcgatg agatctcggt
     9241 gctggtaccc gacgacttcg tcggcgcggt gatgggcgac ctctcgggac ggcgaggccg
     9301 tgtgctcggc acggacaccg ccggccacga acgcaccgtg gtcaaagccg aggtgcccca
     9361 ggtcgagctg acccggtatg caatcgactt gcgctcactg tcccatgggg ccgcgtcgtt
     9421 cacccgtgcg ttcgtccgct acgaacccat gccggaatcc gccgccgcgc gggtgaagac
     9481 ctcagcctga caatgcctga taaggcctga taaggcccga gacggcatga ggcggcccga
     9541 aagcgtttga aaacgcgtga catagggtga gattggtacc tatgtcgacg ttcaacggac
     9601 ttcccgcaca catcctgctc aaccatttcg tcgtcgtcct gggtccgctg accgcgatcc
     9661 tggccatcct ctgtgtgctc tggcccgcgg cgcgacgacg gctgatctgg ctggttctgc
     9721 tgctggccgt gggtacaacc gtcctaaccc cgttgaccac cagctccggc gcgtggttgg
     9781 catcccgcgt cggtacctca cccgagctgg cgacacacga aggactcggc gcgaccctca
     9841 tctacatggt ggcggcgttg ctggcctcgg tcgcgctgct caccgctgtc taccttcgcc
     9901 agacccgcgg acgtaccgtc aaactggccc tgcactcggt ggtcggggtg ttggtgatcc
     9961 tcgccgccct ggccaccctg gtgcagacct accgcatcgg cgagtcaggc gcacgcgccg
    10021 cgtggggaaa cgtcacatcc tccgccccgt gagctcgagc cgattcccat ctaaaccctt
    10081 tgtcgtgcag cacttctcgc ggtctaccag ctaccgccgg ccgccgaacc ggtcggccac
    10141 cgcgcgccgg tccagtgagc ccttcgcggt atgcggcagc gcgccggcct cccggaaggc
    10201 gctgggcacc tcgaaggcgg ccaaccgctc gcggcagaac accgcaagct cggccgcgga
    10261 cggcgacggc gacttccgcg gaacaatcac cgcggccacc gtctccccgt acagcttgtc
    10321 cggcacaccg aacacggcca cctcaagcac gttgggatgg ctcgccagca cgccctcgac
    10381 gcgctccggc gaaatcttct cgccgccccg gttgatgagt tccttgatcc ggccacggat
    10441 gctcaggtcg ccggccgtcg acagcgttcc caggtcgcca gtgcgcagcc acccgtcggt
    10501 gaagttggcc gccgtgatcg ccgggtcacc gaggtagccg cgcaccacgg tggggccgtg
    10561 cagccacacc tccccgacgg cttccgcggg cagcggctgt ccgtccggcg cggcgatccg
    10621 gatctccggt cccgtcgacc ggccgacgag accagttgtc gccgccgggc tttcgtcttg
    10681 atcgacgctc tcgatgcgag tcgtggccac ctggtgagtc gcctcggtca tgccgaaggc
    10741 acacaccacc ggcgccgcga acgtatcgtg cagtgcctga gcggtttccg gcgtgagtgg
    10801 agcgctgcag ctgcggatga agcgcagcgc ggccgagctg cccggccgct cggtcttggc
    10861 gcgctccagc aagatctggt gaatcgtcgg gaccgccgtg taccacgttg cccggacagc
    10921 ggcgatgtcg tcccagaagc tgtgcgccga gaatcgcccc ggcgccggca gtagcaccgt
    10981 acctccggat gccagggtcg acagcaacgc ggcgagcagc ccgtgaccgt ggtacagcgg
    11041 catcaccgcg accgtcgcgt cctgcggccc gagcccgtag ccggcaacga tggcgcgcac
    11101 cgagctggcg atgttgtggt gcgtccacgg gaccatcttc ggagtaccgg tggttccgcc
    11161 ggtgaacatg atcatggcgt cgtcgggccg cagcccctcg ggcgccgaaa cgtcgtgctc
    11221 cagcggcgcg ccggcgtcca tgttgacggc gacggttcct tgctgggcac cggcttcgcg
    11281 gaccacgctc accgcgatcg gccagcgccg caagcccggt tcccgggtgg cttcggcgct
    11341 ctcaggcgcg ttgccgtcga cgagcaccac ccgcgctccc gcggccgcgc ttcggatgca
    11401 ctgctcggcg acgggcaggg ccgggtccaa cgggaccacg atgaggtccg cacgggaggc
    11461 ggccaacaag gcgacgacga attcggcgtt gctaccggca cgcagcgcga cccgatcccc
    11521 cggcagcaga ccaccccgct tcagctgtcc ggccaggtcg tcaatcagcc gcgtcaggtc
    11581 gcggtaggtg acaggtatgc gctcggaggt gacgacaagc gccgctgcct ccggtcgcac
    11641 gctcgccgcc gtctcgacca gatccgccac gcgcgggccc cgggtttcgt cgtcgggttg
    11701 cgccgcgaac cgttcggcca caacgtcgtt gccggtgatc gagtccgacc tcatcgcgcc
    11761 attccttccg cgttgactgc aaagctctcg ctcgcacctt gccgcgccac cgtctgttcc
    11821 gtccaatacc gatcggctca caatcgatag cccacaccta ttgagtgggc gtgccgcacc
    11881 gctgatccgc ggtcgataga cggcgtgaat cgctgcatag ccgcttagtc ttggacacct
    11941 gcgggcacca tgacaacagt gatcggtgag gcattaccgc gcacgcgggt caatctgcac
    12001 acgaggagtc gccaccatgg ccaccgtttc ggcatctccc agcacagctc cggagtcaac
    12061 gcgtctgacc gacggcttcc atcttgtcgt ggacgctctc aaggccaacg acatcgacac
    12121 catctacggg gttgtcggca tcccgatcac ggacctcgct cgcatcgcac aggcatccgg
    12181 aattcgctac atcggctttc ggcaggagac gtcggccggc aacgccgccg ccgctgccgg
    12241 gttcctgacc ggccgccctg gagtctgcct gacggtgtcg gcgcccggat ttctcaacgg
    12301 cctggccgcg ctggcgaacg ccacgacgaa ttgttttccg atgatccaga tttcgggctc
    12361 cagtgatcgg gcgatggtgg atttgcagcg tggcgagtac ggtgagctcg accagctcag
    12421 cgccgcaaag cctttcgtga aggcggcgta tcggatcggg aaggtcgagg acatcgggcg
    12481 cggcgtggcg cgcgccattc gcgccgccgt gtcggggcgg ccgggtggtg tctacctcga
    12541 catccccggt gacgtgctgg gccaggccat ggcggcctca gccgcttccg gcacgatttg
    12601 gcgggtggtc gaccctgccc cgcggcagct accggcgcct gaggcggtcg accgcgcgct
    12661 ggacgtgctc gcacaggcgc ggcgtccgtt gatcgtgctc ggcaagggcg ccgcctatgc
    12721 gcaggcggac agcgtgattc gcgagtttgt ggaggccacc ggaattccgt tcctaccgat
    12781 gtcgatggcc aaggggctgc tgcctgattc acatccgcag tctgcagcgg ccgcgcgatc
    12841 attggcggtc gcgcgcgccg acgcggtgct gctggtcggc gcccggctga attggctact
    12901 cggccacggg gaatcgccgc aatggtcggc cgacgccaag ttcgtccagg tcgacatcgc
    12961 ggcgtcggaa ttcgacagca atcagccgat agcggcgccg ctggtcggcg acatcggctc
    13021 ggtgatgtcc gccctgcgtg acggcctggc cgcgcatccg atcgccgtgc ctgcggaatg
    13081 gaccagcgaa ctggccgatc gcaaggcccg caacgacgcc aagatgcgcc agcgtctggc
    13141 cgaagacccg cacccgatgc ggttttacaa cgcgctcggt gccattcgat ctgtgctgca
    13201 ggacaacccg ggcgtgtatg tggtgaacga gggagccaac gcactggatc tggcccgcaa
    13261 cgtcatcgac atggagctgc cccgacaccg gctcgacacc ggaacgtggg gcgtaatggg
    13321 catcggcatg ggctacgcca tcgccgccgc ggttgagacc ggcaagcccg tggtcgcgat
    13381 cgagggtgac agcgcgttcg gcttctccgg catggagatc gagaccatct gccgataccg
    13441 gctgccggtg accgtcgtca tcctcaacaa tggcggcgtt taccgcggcg acgaagcctc
    13501 gcctgggggc gacccggcgc cgaccgtact caatgcgcgc gcacgtcacg agctgatcgc
    13561 agaggccttc ggcggcaagg gctatcacgt caccacgccg gcccagctgg ggtcggcgct
    13621 ggccgaagcg attgcctcca acgggccgtc gatcatcgac tgcgagcttg atcctgcggc
    13681 aggggtggaa agcggacatc tggcgagcct caacccgacc agcgcggcca caccgccgcc
    13741 ggcggcggtc aacgtcggcg cgtgataccc aacagctgcg cgtcgaagaa ctcgttcagc
    13801 gccagctcct gtgcgcatgc ggcgagggcg cgggcgattg gcgaccccgg tccgcccgaa
    13861 ttggtggcca gcgcgatctc ggcctgcacc accggatcga ccatttcgac ggcgcggatt
    13921 cccgcgccca acggcgacgt ccacagccag gtatgcggaa cgatgcatgc ccagtttccg
    13981 gtggaaacct gagccaatag cgaagcgacg gagtcggttt caacctgcgg agcgacggtg
    14041 atgccgtggg cagcgaacgc ttcgtcaatg atctggcgat cccgcatgcc ggcagacagc
    14101 aacgccaggg gcaattgcgc ggcctccggc cacgtcagtg tcgacgcgcc ggaaggcaac
    14161 atgtccgtcg acgacagcag cacgtagtgc tcctggtaga gcggcaccaa gctcacctca
    14221 tgaccgtcat cgggtgcggt gtgcactatc actgcgtcca ggtcgaattc gcgcagccgt
    14281 cggtacagct cggtggcggc cagccgcgac aggatctgta ccttcgccag tggatgcgcc
    14341 gaacagaagg ccgagagcac cagggaagcc gtcgttgagg ccgtcggaac cgtgcccagc
    14401 cggagcgtac cggtgatacc cgaccgcacg gcgtgcacct cggccttgaa cgagtcgtgt
    14461 tcggcaagga tccgcttggc ccacacgacc aatcgttccc cttcgggagt gagaccttcg
    14521 aagctgtgcc cacggttgat cagcgtgacg ttcagctcgc gttcgagctt ggcgatcgcg
    14581 gcggagagtg cgggctggga gacgtagcac ttctcggccg ccctggcgaa gtgacgctcc
    14641 tgggcgactg caacgaagta ctccagttga cggaagagca cccgcacctc ctcggcctcc
    14701 gcgcaccggc ctcgctgagg ctaacgcgtc gcccgcacgt cggccaatcc ggcgtgtcgc
    14761 acaaggccgg acaatgccaa aacgtttact tcgacaggtc gcgaagtcgt gtccggatgc
    14821 gtcccgaaag atccgaccgc gacggcagtt cgaacggttc gccccgtgaa actcctggta
    14881 ttggaataac tttcgtgact gctctggtca acaggggttt cccggcaggc gcggccccgg
    14941 cgctggttac gcatgggcgg taatcgcgtt ccaatgcaac cacattggga acactcggca
    15001 tgttgggcaa tgctagcggc gacccggccg gcggcgctca ggtccgcgcc tgacccgcga
    15061 cgtccgtgat ggaattgaac caaaccggca atcggatcgt tatcgtggcg agcggacgaa
    15121 gctgcaatcg tttgctgaag accgtaatgg gtagacagcc agtagaatac cgttgtgttg
    15181 actgcaccgt ctcaaagggt gaacgccatg cgtcgagcgg ttcgttatct attcgtcatg
    15241 gtggcgatca cggccatggg cgcgttgggg agcggcacgc gcagcgtggc cgcggtcccg
    15301 gtacccgcgc cggttcccgg ggtggcatcg gtcttgccgg ctgacggcgc gctggtcggc
    15361 gtcgcccatc ccgtcgtggt cacattcacc acaccggtca gcgaccgcgg caccgcagag
    15421 cgctccatcc atatcgcgtc gccgagcaat acgcaaggcc atttcgagtg ggccgaaagc
    15481 aacgtggtgc agtgggttcc gaaccactac tggccgagcc atacccatgt gtcggtgggc
    15541 gtgcaggccc tgacgacggg ctttgacacc ggtgacgagt tactcggtgt cgccagcctg
    15601 tccgcgcaca cgttcacggt cagccgagac ggagaggtcc tccgcacgat gccggcgtcg
    15661 atgggcaagc ccacccgccc gacgccgatc ggcgacttca cagctctgga gaaggaccgc
    15721 acggtggtga tggactcgcg gactatcggt attcccctga gttcacccga gggatacaag
    15781 atcaccgccc aatacgcggt ccgcgtcacc tggggcggtg tcttcgtgca ctcggccccc
    15841 tggtcggtcg actcgcaggg ccacgccaac gtcagccacg gatgcatcaa cctcagcccg
    15901 gacaacgcgg cctggtactt caaccaggtg aacgtcggcg accccatcga ggtcgtggcc
    15961 tgacggcgcg cggcaggttc gccgcgcctc ggctcggtta gctgggcaac ccgctcttga
    16021 tcgcggcgtc ctgcttgcgc ccgacatcgg tcgcaacggc ggcatcgatc ggatcgtttg
    16081 gcgcgctgtc gaattccaga ttcacgagcg ccttgccttc ggtgaacaac agcaccgtca
    16141 ccgcctgaga attgtcgggc gaggtaccgg aaatcattgc tccattggaa ccgacgttga
    16201 ccggctgcca cgtgccggtg acccggcccg cgtaattggt cttggtgttc tcgagccctg
    16261 cgaccgcggc cgcgggatcc gcgacgatca agatggtgtc cccgattcgt cggctgttgt
    16321 cggcgttggc gaaaagctgt gcgacgcccg ccgcattgtt ggggttcagc acgggtggtt
    16381 ggggggtcgt cagatctccg ccgatatcgc tggccttgat cagcagcgaa ctgtaatcgg
    16441 agggttgtcc cggcgccgga ctcgttaccg tgctcgaact ggtcgaggaa gtcgcggaac
    16501 tcgacgacga aggtgacgac gcattgtggt cgttgccgca gccggcggtc gcggtggcga
    16561 tcaccgcggt ggcgacggcc cagccggcga ccgtgattcg ggcgagctcc atgggcattc
    16621 cttccccgtg aatccatgcc tggcagcgac gataggcagc cggttcggcc gcggcgcctg
    16681 acgctactgc gaaatgccgc cgggaacaag gccggcggct cgcgacctgg gcggacaacc
    16741 ccgccggtgg cgccggtgcc cggcctatgc tggcgtgggg ttgggcatat tcgcggggta
    16801 aggagagcgc aatgacggct ggggcgcgtg ccgtacggtt cgaccgctac gggggacgtg
    16861 acgtgttgta cgtgacggac atcgacatgc cgtcgccagg cctgggtgaa gtggtggtcg
    16921 aagttcgggc cgccgggatc aatcccggcg aggccgcaat ccggaccggg gcgctgcgcg
    16981 acatctttcc cgccaccttt ccgtccggtg agggcagcga cctcgccggc gtggtgaccg
    17041 ccatcgggcc cggggtcacg gagttctcgg tgggcgacga ggtattggga ttcagcttcc
    17101 gccggtccag ccacgcgacc cacaccgccg tcccggtgga tcaattggtc cgtaagcctc
    17161 cccaattgag ttgggaggta gcaggttcgc tctacgtggt ggccgcgacg gcgtatgccg
    17221 ccgtccgctc ggtctcgccc cagccgggtg atacggtcgc cgtctcggcc gccgcgggcg
    17281 gagtcggaag tcttgtcgta caactgctgg ccttgcacaa ggcacgggtg ctcggcatcg
    17341 caggaccagg caacgccgac tggctgcgcg cccatggcgt catcccgatc gcctacggca
    17401 acgatctcca agagctggcc acccggctgc gggaggcagc gccggaggga atcgatgcgt
    17461 tcatcgactt attcgggccc gactacgtcc agctcgcggt ggacctcggc atcgcacccg
    17521 agcgaatcga caccatcatc gcgtttcaga aggccgtcga agtcggcgcc aagaccgaag
    17581 gcagcgccga ggcgtcgaca cccgaagtgc tcgccgaaat ggccgatctg gtcgcgaccg
    17641 gcgccctcga tttcgatgtc gccgccacct atccgttgga ccgggtcgcg gacgccttcg
    17701 aggaactcga acggcggcac acccacggca agatcgttct gctgccgaat ggttcgcagt
    17761 agcggccggc tcgcttcggc gatccaggtg ccgattcagg cgatacccac ctggctaggc
    17821 ggcgagcgcc ttatcggcgc ccgccaccga gccttgacgg ggaaatcacc ggcggccacc
    17881 cgcactgtcc gacgggtgtc cacccagcga ggcggtgatg acggcgatcg gcatccgcga
    17941 gacgtacagg ttggcccgct cctgcggagt cagcctgcgc cgcgcgtgca gcttgcccgc
    18001 caacagccgc cccgagtgca gcaatgaccg gccgcttccc ctggttagtc cggaaacacg
    18061 gtgggtaaca acaaaagcca tgatcttctc ctgtcgaaaa ggcccgatcg actcatggca
    18121 ttcggcgcgc agcacgtgac gtgcaactgc gcaacgcgct tagcagcgga gaacgagcgc
    18181 ccaggacttc aggcggcgcg aagaatgccg gaaccggatt gggacaagac ggatttcgga
    18241 tagggactat cgccgggact catctcgccc tcacctcctc acggccagga cacacgcggg
    18301 tgtcaccaca ttcacctgaa ccacaagagg aacgaacgaa aggcggcgga ccgccgatca
    18361 cacccctctc gtcggagctt cggcactgca cggcgtatcc ggaacttcgt ccggaagcca
    18421 cttgggctca acccttgggt ccgggaagag ctgtcctgac ccggggcgtc tctcgacgtt
    18481 cggggtcagt ggcctgtatc cgtgcagacg cctcacctac cgaggtgttt gcaaagccat
    18541 gctcgcaccg ccacccaacg ctcgtcaaac tccttatcgc attccttacg cgaacgatgc
    18601 cgatcgcatg tcctgactca cattcgcctg cctccgggcg ccgtccggcg gcatcccctc
    18661 gaggcttacg ctcgaaacat gccgacaact ttgcaggacc ccaaggtcgc cgcggtgctc
    18721 gaccggatgt acgccgaatc gcaagcccag atgtcgctgt tgcgtgagcg acgtggtcag
    18781 ttcgagcggc cgatgacgac ggcggaacgc accgaggcga tgagcgagtt ctacattccg
    18841 gtgacacccg aagccggccg gctgctctat gcgctggtgc gggcgacccg accaacgacg
    18901 gtagtcgaat tcggcatgtc tttcggcatc tccgcggtgc atctggcatc ggccgtgcgc
    18961 gacaacggca ccggacgcgt ggtgaccacc gagctcagcg acaccaagat cgccgccgcg
    19021 aaaaagacat tcgccgagac cggtttggac gatgtcatca ccattctcga aggagatgcc
    19081 ctgtccacac tggctagcct cgacggaccc gttgatttcg tgctgctcga cggctggaag
    19141 gatctctatc taccggtgat gaagttgctc gaaccccgcc tgcccactgg tgccttgatc
    19201 gtcgccgaca acaccagcgc ggccgacacc gggccttatc tcgacctcgt gcgcaatccc
    19261 gacaacgggt acaccagctt caacttcctc gtccgcgaca gcgacagcat ggaaatcagc
    19321 tgccgcaccg acgattaacg cctaccgcag ggattcctcg agccaaggcg tcagccactt
    19381 caccccttcg ggggtgaggt gaacgccgtc gctgcgcacc ttgattccgt cgaccttggc
    19441 ggtgtaaacg ccgtccggac atagcttctt gttcaagtcg aggatcgcaa cattgggatg
    19501 gtggctaacc gtgttgcgta gcatggtgtt ccacaggttg acccggtccg gctggtcctc
    19561 ggggtacaag cggccgtccg ccttttcgcc gcccctgctg tagggcacgg tggccaccac
    19621 cacgcgaatc ccggtggagc tcacgatatt cagggcacgc tcgagttcgc cgttgaggta
    19681 tgcgtcgaac gtgggatcgc cgatatgggt ccactgcccc tcgttgaccc ggtcgacggt
    19741 ctcccagcgg ccgataatca acagcgcgac gtccggccgg tcctggttga cctgcgttga
    19801 ccacctgatc ggccagccgt cgcattccgc ccgctgctcc agggtttggc cgatgtaccg
    19861 atacggtgtg ccgcgcacca ggctgcagcc gatgaccgtg tggtcgagga acgtgaatcc
    19921 gggcgtcggc ggaaggtaat gcatccaggt ccacccgatc gaatctccga acaccgaaac
    19981 ggtgaacggc cggttgggat ctcgtggccg tgcgcgactc tcgcccggcg gcgacggcga
    20041 caccgccgcg accgccgaga cgccgggcgg caggcctacc tcacgcaggc cgggtccggt
    20101 tcccaccgga atcaccagca gcgtgaccgc ggcggcgctg gccacggtgg ccgccgccag
    20161 cggcaacaac ggcacccggg ccggccgcca gcgccggatc ggttgttcga tgagccacca
    20221 cgatgcggcg gccaccacca ccgtggcggc acaccgggcg ccgaacagcg gcagcccagt
    20281 ccacccggta cgttcgccgt tgagcgccag aaagattggc caatgccaca gatacacgcc
    20341 gtaggaaatg gtgcccagcc ataccagcgg cggcacggcc aggacgcggg caatcgctcc
    20401 gcgctgctcc actgccaccg aggcgacgac ggcaacggac gcgatggcga ctccggtcag
    20461 cagtccgtgg cgaaattcac cgacgctccc ggttgcgtag tgagtggccc ccgccagccc
    20521 cgccacaccg acaaacggca gcaagcgggc aacccgccgc ccccaccgcg tccggatcat
    20581 gcaccagccg cggttcagcg acggccaatc ccgaaccagc agagccgctg ccgccgagcc
    20641 gatcagcaac gcctgcgcac gggtgtcggt gccgaaatat attcggtcac gagtgccgcc
    20701 ggaaacgaag acgatggccg cggcggcgga agccagcgcg cccagtgtgg caatcgcgaa
    20761 gacggcaaag cgaaccccgc ccacggtggc ccgcatgaag tagcgcctgg cccgcgccgc
    20821 caacagcacg gcaaccgcga tgagcagcac cggccagaca aagtagtact gttcctcgac
    20881 gccgagcgac caggtgtgct ggagcggcga cggtggagcg ccttgggtga agtagtcggt
    20941 cttctgggcc acgaaccgcc aattcgccac ccagaggaat gccgcgatcg cgtcgttgcg
    21001 taacccggtg agggcctgag tcggaagaag ctcgcgcgcc gcgcccacgg tgagcaccat
    21061 caagaccaac gccggcaaca accgtcgcgc cctgcgaatc cagaagccgg tcagctcaat
    21121 acgaccggtg cgtcccagct cgtccagcag cagcgacgtg atcaggaagc cgctgaggac
    21181 gaagaagacg tcgacgccga tgaacccacc gcccacaccg gggatgccgc cgtgcccggc
    21241 gagcaccaac gcaaccgcca ccgcgcgcag cccatccagc gcgggaatcc cgctgcgccg
    21301 ctccggttta tcccaaatcg ccagccgacc ggcgcggcgt acgggaacga cccatcgtgg
    21361 ccgccgagca gaatccgccg gcgcggagtt cgtcccgccc ggctcagtgt tcgctgttag
    21421 cgcgccctga ccaccatctc gtccggcgcc atcgttgctg ccgatacgtc gctgcccctc
    21481 ctcttgatcg aagcaagctt agaggcgacg ctgccggatg caggcgaaga cacgcgaaga
    21541 cacgcaacgg ttttcccgcg gcgacgatgt cggctcgggc taggcctgac ctgcgggttt
    21601 cacgtcgcct cgcacgtcgg cgaggcccaa tccgagttct tcgagaacct ctgcggtatc
    21661 ggctcccaac tccggcgcgg gacccgcaac atggcccggg gttcgggaga accaggtggg
    21721 cacgcccggg aaccggaccg ggccgtgcgg cgtgtccacc gtctcgaaaa agccgacggc
    21781 gttgaggtgc tcgtcgtcga acaggtttgc cgggctggac agagacgcgg ctggtatctc
    21841 caatcggcgg aacagttcga tccattcgtc tgtggtccgc tgggcgaggg tctcggcgag
    21901 cagggcgtag accgtgtcga tctggcgcgc acgtcgctcc agcgtggcat acaggtcgct
    21961 ggcccagggg ggcttcacgg cgtcgatgaa cgcggcccaa tgcttgtcgt tgtagatcaa
    22021 cgcggcgacg tagccgtcgc tggtgcggta ggggcggcgg ttgggtgcca ccgtgcgcgg
    22081 ataaaccgcg gggcccagcg gcgggtcgaa catggcaccg ttggcgtgtt cgaccagcat
    22141 gaacgacgcc agcgtctcga acatgccgac ttccacctct tgtccttcgc cggtgcgctc
    22201 gcggtggaac aacgccatca tggtggcgta gagcgcggtg agacccgcga ccttgtcggc
    22261 caatatcgtg ccgacgtaat tgggctcgcc cgtcaactgt tcttggacga acggcagccc
    22321 gcacgcggcc tggatggtgt cgtcgtaggc cggcagatca ccgttgggcc cgcgccggct
    22381 gtacccgtaa cagttggtgt acacgatgcc ggggttgatc gcggccacct cgtcgtagct
    22441 gaatccaagg cgggcaaccg ccttggaccg catcgagtga atgaagacgt cggcctgctc
    22501 gatgagcgcg cgcaggacgg acttacccca gtcggactgc aggtcgaggg tgacgccgcg
    22561 cttgccgcgg ttgacgttga cgaagactcc gcccatgccc cggaccggcc cgaccgagac
    22621 gtagcgggtg tcgtcgccct ttgggggctc gaccttgatc acgtcggcgc ccatgtcggc
    22681 catgatctgc gtgcagtagg gccccatcac catcgcggtg aggtcgacga cgcgcacacc
    22741 cgccagcggg ccgccccggt tgctggaatt ggggaggcga ctaggcatgg cgcacccctt
    22801 ccttcctcag cgcggtctcg aaactgtagc ggatgtgatc ggcatgcccg tcggggacga
    22861 cgggaaagac cagcggcgcg gcggcgcagc ggatggcgtc gagctgctcg gccacatcct
    22921 cgatcgacca cgacggacgc tgcaccccag gggtttccgc gacgaccact ctggccaccc
    22981 ggcccgcgat cgcaatcagc atctcaccgg tgatcgggca ggtttcgtgg gccagtaatc
    23041 cgaccaccgg cgcaacgagc tcggtgccca tcggcgggta tgccgaaata tccaggcctt
    23101 cagccattct cgtcaccgcc gagggaacga tgacgttgca gagcacgccg tccgccgcac
    23161 cttcgagcgc gacgacgttg gccagaccca ccaggcccgc cttcgccgcg gcatagttcg
    23221 cgacaccgtg gttgccgtac aggccgccaa tcgacgaggt caacacgatc cggccgtaat
    23281 gcgcgtcgca catgatcggg aacgccggtc gcagcacgtg gaaggcgccg cgcagatgga
    23341 catcgatgac ggcgtcgaag tcctggtagc tcatctcctt cagcgagcct cgccgtacgt
    23401 tgccggcatt gtggatgagg atgtcgatgc gtccgtagcg gtcctgcgcg gcgccgatga
    23461 tcgcctggcc accctccgcg gtcgcgaccg actcgacgca ggcgaccgcg tcgccgccgg
    23521 ccgccctgat ctcggcgacc acctgttgcg cagggccggc gtccgtgccg gccccggtca
    23581 gggcgccacc gttgtcgttg acgacgacct tggcaccccg ggaagccaac agcagggcgt
    23641 actctcgtcc cagtccccgc ccggcgccgg tcaccacggc tacccggttg tcgaatctgc
    23701 gttcaaccgt cacgatccca gcaccaaacc atccagatca ccctgggcac gccactcgga
    23761 cagcagttcg ccgaagacgt aaaagcctgg gccatagggt tcgccgatgt gagaacggat
    23821 gccctcgcct tcggcgaccc cggcgccgcc gccctcgttg ttgtagtagc ccggtgtgca
    23881 ctccaggtcg aatcggctgt tgtcaatcgc gttctcgcgg atggctttac accattcgtc
    23941 ctgcgcctct tgggtgggtt ccaccgtgag cgcgccccgg gtcagggctt cggagatgat
    24001 gtaggcgatg tgctcgccct gcagttcata gttggcggcg atgttcgcgg agatgcccac
    24061 ctgagtgaac ccggtgaaga actgattggg gaacccgcgg ctggtcatcc cgtgcagcgt
    24121 ctggtatccg ctacgccagt ggtcgaagag cgacagcccg tcccggccct cgatcgcgtc
    24181 gatggagtac cggcggctga tctcggtggt gatctcgaac ccgctggcgt agatgatgca
    24241 gtcgacttcg tactctatgc cgttggcgac caagcctttt gccgtcgccc cctcgacgcc
    24301 ctttgtcgac gacacgtcga ccagcgtcac gttcgggcgg ttgaaggtgt gcaggtagtc
    24361 gtcgttggtg cacggccttt tgcacaggaa ccggtagtag ggcttgagcg catcggcggt
    24421 cccggggtcc ccgacgatgg cgtcgacgcg gcgccgcagt cgctccatca ccttgaagtc
    24481 ctcttcttcc cggatggcca tgaactgctc gggggtcagg gctgccgggt cctcgagcgc
    24541 catgacccgc gcggcggtgt tgcggccgag ttcggtccag aagtcgcaga cgtagtccgg
    24601 ctggcccagc gccattcctt cgaacgtcca ggcgtggaag ttgcgctggc gctccttctg
    24661 ccagccgggc cttagcgttt tcacccactg gggatccgtc ggagtgttgt tgcgggcccc
    24721 gacggacgac ggtgtgcgct gaaagacgta gaggtgccgg gcataccggc ccagataggg
    24781 cacgatctgg atcgccgtcg caccggtgcc caccaccgcg acgcgcttgt cggcgagctt
    24841 gtccagtccg ccggaagagt ccccgccggt atatccgtag tcccagcgcg acgagtgaaa
    24901 gctgtgtcct tgaaaatcca tgatcccggc aatccccggc agcttcggcc ggtgaaaggg
    24961 tccggaggcc atcaccacga accgcgcccg gatgtcgtcg tcccggtccg tgccgacgcg
    25021 ccagcgcttg atggcctcgt cccagtgcag ctcgcggacc tgtgtggaga agatcgcgga
    25081 atcgtagagg ccgtagtgct ttccgatgcg ccggcaatgg tcgtagatct cggcgccatc
    25141 ggcgaacttc ttcgacggga tgaagttcag ttcttcgagc agcggtatgt aacagtacga
    25201 ttcgttgtcg cactggatgc cggggtaacg gttccagtac cacacaccgc cgaagtcgcc
    25261 gcccagctcg atgatccgga cgtcatcgac tcccgccttc ttcaggtacg cggcgcacag
    25321 caggcccccg aagccgccgc cgagcaccgc gacatcgatg tcctccgata tcgggtcgcg
    25381 aaccaacggc ggcgagtacg gatcggtttc gtagtacccg gcgaagtcgt cgaccagttc
    25441 gacgtactgt ttggaccctt ccggacgcag ccgcttgtcg cgctccccgc ggtacttttc
    25501 gcgcagcgct ccgatgtcga cgtcgtccgg cgtctgagtc ggcgggcagt ccggcctcga
    25561 aatatcagtc atgccagagc gttctccggt agcgccgcgg catgcagggg cgcctccatc
    25621 ttctgcacgt cgggcagcac ctctttggcg aacaactccg tgctcgccat cgcctcgggg
    25681 tagggcatcg tgccgaattg cggcacgatc gtcagctcgc agaacgagca cgcctcctgg
    25741 gcggccttga tcctggtgaa gatctcctcc ggcgtcccga tcagcaggtt ggacgcgtga
    25801 tagcccgggg ttttgggctg ccctcctttg gccgctccgg tcaccgacga cgcgaggacg
    25861 gccgtcgcgc tggcctcccg ggccgcatag gcttcgtacc ccttgacgcc cttgaagttc
    25921 gaggcgtcgg cgaatccgta gtgcacggtg acatcccggt tggcggtcca gatccattcc
    25981 tcagacctgg cgggatcctc tgtggtgcag tacatgaaca tcacgttctt cggctggcag
    26041 ggcggcaggc cctcctcctt gcggaatatg ttcacctgct gcacttctcg tccggcatcc
    26101 tcgatcggct tgtttcccac gaagagcggg accatgccgc ggcgggcgag gatctccagc
    26161 gattccgcgg tcgacgatga gctgtaaatg cgcgagaaca ggtcgcgggt ccggggttcc
    26221 ggacgaagcg acatctcggg aaccgtgaag atctctcctt cgtaggagaa ccgctcgccg
    26281 gacagcgcca gctgcaggat gtcgagggtt tcgttgaagc gctgcctgct ttcctcgcgg
    26341 gggacgccga cggcgtcgaa ctctcctttc gacaccccgc gcccgatccc gatcgtcgtg
    26401 taccgcccgt tggagaggat gtccaggtag gcgatctggt gtgccaaccg gatcggatgc
    26461 caccacggcg cgacgacgac gaaggtgccg aggctgacgc gttccgtacg accggcgaaa
    26521 tagctcaacg cctgaatcgg gttgggcgtc atgccatacg gggtcccgca gtgctccggc
    26581 atccagatgc cgtcgaaccc cagcggttcg gccagatcgg cgatcgcgag cgtggcctgg
    26641 acgcactccc aatcggcggt gacgggcggg tggctgaagt ctccggccag cacccgttcc
    26701 cagtcgtggg agttgtgcga tccgaaccca aggttgactt tcattcccct gtctctcttt
    26761 tcaggtcagt ggccagcttg ctgggcgcac cggtgcccag gtattcggcg aggttacggt
    26821 gcagatttgc ggtgctgcgc tccatgtagg ggttgggctt cgggccggag aagcccagcg
    26881 acttcatgcc ttgctggacg gcggccatgt tggagaagtc ctgcggcagc acactgcgcc
    26941 aattcgggtc gcccggcggc gcgtacaccc attcggtttg cggctcttca cctttgggat
    27001 agagttcgta gcacgccgct tcgaagatgc agctatcggg gtcgtagccg tggggccggg
    27061 cgctgtagca cagcgcggtg gtcaggccct gcccgatctg aaagttggga aatatctgcc
    27121 aggcggtgcc gctgcgggcc aggtgttcgg gatcgatcgt cggccaggtc acaccacgcg
    27181 cggcatcgtc acgtcgcgcg gatgcgagcc aatgctcgag cacctggccg gccggtgcac
    27241 cttcgggtag ctcgtcgacc agtcggcacg cagcttccac gagtgttttg gtggtcgtgg
    27301 cgttggtttc gtccaacgtg tagacctgca tctcggcggt cgagatgcgt gggtccgcgc
    27361 ccgttccgag ccggatcttc gatttggttt cctcgaggtc cgccggcgcg tcgtagccga
    27421 tattgctgtg cttgccgtgt gccacggccc aaccacggaa gttgccgaac ttggtgaact
    27481 gcgggtgtgt ggtcgcgacg tggtaggtct cgttgaaggc ttccatggca accttccagt
    27541 tgcacccgaa atgcagccat ttgcgccatt tgcagcgcat gtcctgcagc tggaagggat
    27601 cgagcatggt ggctgcgggc tcgaggtagt cacgcagcgg ctcacaaccg ggatccatgt
    27661 tgacccagat ccagccgccc caggtgtcga cgttgacggt tgccagatgg gtgttctccg
    27721 gcgtcagggc gccctgccag tcctgcttct cgggcacgtg gatgcagttg ccatcctggt
    27781 cgtacgtcca gccgtgaaag ccgcacacaa acgacttctt gtggccgcaa gcacttttcg
    27841 ctcccgccgg gacgtccacc agccgacggc cgcggtgcat gcagacgttg tgatgcgccg
    27901 ccaaactgtc gggcccggtc cgcaccacca cgactgaatc gtcgagaatg tcgtaggtga
    27961 ggtagctgcc caccttgggg aggtcttcga tccgcccgac ttgctgccag acctttcgcc
    28021 acaacttgtc gcgttccgcg tgggcgtact cctcggagat atatgcctcc accgggatcg
    28081 tgaccggcgt ggccagctcg tcggcccggt ccatcgcgtc ctcgcttcct aatcccattg
    28141 ccgctcaggc cccgtcccaa cgtgtaatcg ccgcgcggaa ggtttcgtcc ttgaggaaca
    28201 gagaggtgtt gtcagcgccc aggtgccccc atttgaggtt caggccgccg tccacgagca
    28261 acgtctgtcc ggtgatgtag ctcgaaagat cggacagcag aaagagaatc gctccggcca
    28321 cctcttccgg gcagccacgg cgtcccatcg cgatggcgtg gcggtcgcgt tccgggtcgt
    28381 cgtcgacgta cgtgcccgac gccggcgtcc tggtcacccc gggtgccacg gcgttgaccc
    28441 ggatgacggt gccgcgcgac ccctcgtcgc cggccgccag ttcgagcgcc atggtgcgcg
    28501 ttaccgccac gatcgcggct ttggcagtgc cgtaagcaat gtggaagggc gccgtgttca
    28561 tcccgctgat cgacgagacc gaaacgatcg agcccccaca cttgcgccgc ttcagttcgg
    28621 cggcgacggc ctggctcatg aagaacatcg tctccagatt tcgggtgaac agttcgcgcc
    28681 agtcactgcg cgtcacccgg gtcgacggca tccacgtcga cggatccgcg ccacccgcga
    28741 cgttgaccag tccgtggagt tcgccctcgg tgcgccgcac cgcctcgagc gtggcgtcca
    28801 ccccgtcgtc ggtggacgcg tcggccgcca gcggcacgac cgacaaaccc tcggccagca
    28861 gtggcttcac atgccgatcg agattgtctt gatcgcggct caccgcgacc acggtcgccc
    28921 ccgcctcggc gaccatgcgt gtgacggtgg taccgatgcc gccgccggcg gcgccggaaa
    28981 cgacgacaat gcgcccgtca agcctgagca ggtcccgatt catcggctat cttataattg
    29041 tccggacaag ataatgacgt gcattcatta gagagcatga ttctctgcgc atagcgcccc
    29101 cgtcaagggc ccgcccgctt cggggctagc tattgtctgg actattcgcc gattgctatc
    29161 gtgcgagcgg tgatagccgc aaccggattg gcgccgcacg gcctgtctca gggccgctac
    29221 cttgccgcgc ggcagcccag cctcgccgag ggctggagcc tgacgcggct cactccgccc
    29281 agccggctct tcggggccaa cgggctgcgc accggtccgg acggccgggt ctacatcgcg
    29341 caggtgaccg gcagccagat cagcgcactc gacgtgacga ccggtcggct ggacgtcatc
    29401 agtgccaagg gcggggagat cgtcgcgccc gacgacgtcg ccttcgaccc gagcgggaac
    29461 ctctacgcca ccgaggtgat ggacggacga gtcagcctgc gcgacgccgc ggggcgcacg
    29521 cgggtgctgc gcgccgacgt gccgtcggcc aacggcatca cttttcaccg gggccggttg
    29581 ttcatcggcg agtgccgggc gggcgggcgc ctgctcgaac ttgacctcaa cggcggtgcg
    29641 ccccgagtgc tgctggagaa cgcgccgtcg cccaacgcga tggaggtcgg gcccgacggc
    29701 ttgttgtatt tcccgttgat gggggccaac gagatctggc gcatcgaccc cgacggcggc
    29761 gagcctgaac gagtggccgg cgatctcggc gtcccagacg cggtgaagtt cgactccagc
    29821 ggccgcatcg tgtcgaccca ggtgcacagc ggtcaggtgc tgcgcatcga tccccgcacc
    29881 ggcgatcgca ccgtgctggc agccctgaat cccgggctgg acaacctcac cttcgtgggc
    29941 gagcggctgt ttgtctccaa cttcaccggc gagatcgccg agatcctcgg cggtggccag
    30001 gcccgcaccg cgctgccggg cggcatgaac tggccgctgg atctggcggt tgataccaac
    30061 ggcgacctct acgtcgccga cgggacctac ttctacgctt tgggcgccgg cgggcgaagc
    30121 gggtcgtcgc ccatcaggcc ccccacactg cggaccgtcg gaatgctgtt cagcccagga
    30181 tatccgggct tcttgcgcgg gctggccgct accggcgccg gcgagttcgt cgtcaccacc
    30241 tccaatggcc agctcgctcg gtatcggccc ggtagcaacg aaagtgaagt gctggcagag
    30301 ggtttcgacc agttgtatgg cgtggcggtg gctcccggtg gggccatcgt ggccgccgaa
    30361 ctgggtgcgg gacgggtgct ggcaatcagg tccggcctcg tcgaggtgct cgcggccgga
    30421 ttgaacgatc cgattggcgt ggccatcgct cccgacggcg cgtgcttcgt cgccgaagcg
    30481 ggagctggtc gggtggtgag gataggcgga acgggcgcaa gaatcaccac agcgttggac
    30541 ggcttgcggc gaccccatgg catcctcatg ctcgacgggc ggctctatgt cgtcgatgtc
    30601 ggcgcgcagg agctcatcga gttcgacctg gcgagcggtg accgcggcgt cgtcgcgtcg
    30661 gacctgccgg taggcgcgcc gccgggcgtc gtccccaagc cgctgctggg gatgccgccg
    30721 ttctccgggc cgcaggggcc gttcgccggc atcgccgcgg gacccgacgg gacgctctac
    30781 gtatcggcgg acgcagaggg cagtgtgctg gcgatacacc gggaaggcgc tgaggactga
    30841 attgtcagaa cccaaagttg tggatcatcg ctacctgcag gtcgcgcgta ccctccgtaa
    30901 ggagatcgtg gacggcgtgt atccggtcgg gtcgcaattg ccgaccgaac acgagctgtg
    30961 cgagcggttt gcggtgagcc gctacacaat tcgtgaggcg ctgcggcggt tgcgcgaaga
    31021 caacctggtg tcgtcgcggc cgcgcaccgg aaccctcgtg gtgccgcggc cgtcgacgga
    31081 ctcctacgtt cagcacgtca tgtcgatcaa cgacctgctg gcattcgcga ccggcacgca
    31141 ctttccgatc gaatccgtcg cgatggtcag actcgacgac gagctggcgg cacggacggg
    31201 gctgacgctc ggcgagcagt ggctgagcgt tcggggtttc cggcgcgccg agggggcaca
    31261 gtccgcggag cccgcattgt gccgcaccga gtactacatc aaccgcgcgt ttgcggcggt
    31321 gggcaggctg ttggcgcgcc acgaaggccc gatctttcct ttgatcgaag acctgttcgg
    31381 cctgagtatc gttgaggttc atcaggaaat cgcggcggta ctcattccac cggcactggc
    31441 ggacggcctc gacgtcgagg ccggggcacc agcgctgcag gtgcggcgca cctacacgac
    31501 gtctgacggg cagatcgccc aggtcacgat caacacccat cccgcgtccc gtttccgtca
    31561 ctcgatgacg atgcggcgca tgaaaggcta gccggcacct atatgtcaag gaagccatgc
    31621 cgagacaccg tgaacgaaag ctgaccggcg gacggtcgat tcgctgggac gacgaacgcg
    31681 ccgccgacgc ctatgcacgc ggctggtggg ttcggggcac gttggccgac tcgctgcgcg
    31741 acgcggcgcg gcgcacgccg cagcgggtgg cgctggtcga cggcgatcgg tgggtggact
    31801 gtcaaagcct gtgccggcag gcgacaacac tggccaatgc tctgcacgcc cgcataccgc
    31861 cgggcagcgt ggtgtctttc atgctgccga attggcatga ggccgcggtg atctacctgg
    31921 gcgcgaccat ggcgggcatg gtggtgaacc cgatcctgcc ctcactgcgg gatcgcgaac
    31981 tggcgttcat cctgtccgac tgcgacagcc gcgcgatctt cgtgccgtct tccttcggaa
    32041 agcacgatta cgtcgccatg ctggaccgtg tcaccaccga catgacgtcc ccgccggagg
    32101 tcgtggtgtt gcggggcggt gacgaacgtt ccttaccgct cgaaaaatcg tcgaaatttc
    32161 gagcggtaag gaacgttcgc ggg
//
LOCUS       DASSGQ010000113         2287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_767145,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000113 DASSGQ010000000
VERSION     DASSGQ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767145"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..1921
                     /locus_tag="VFI55_05390"
     CDS             263..1921
                     /locus_tag="VFI55_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015575001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PecA family PE domain-processing aspartic
                     protease"
                     /protein_id="HET7073502.1"
                     /translation="MSFVITAPEMMTGAATDLASLGSAISAANAAAAARTTGVLAAGA
                     DEVSAAITTLFSQYASAYQAMSAQTAAFHAELVHTLNASANVYSAAEAANASPLQTLE
                     QDVLGMINAPTNTLIGHPLIGHGADGTTNAQGAGTPGGPGGLLIGSGGNGGNSTAAGA
                     PGGAGGPAGLIGPGGSGGTGGWGARGGVGGTGGWLWGKGGTGGIGGPLALGGTGGSAR
                     FFGVGGTGGMGGEFGPGGAGGRGGFLIGNGGMGGTGGVVSGTGGPGGQAGMLGAPGPA
                     GAAGGAPIVPLQSQDERLIADVSVGGAPSVPVIVDTGSTGLIVPPQDVNMQALGAVTG
                     HGSVTYGEPGNTLTETYNTYTTTVNFGNGITTAPTSVAVVTSVISNGTSYSTSEAPAI
                     LGIGVNAGGPTTSPVPALPGGLGQGVLLDEPGGVMEFGANPLPAYASVSGAPVTTLDV
                     KINNGTLTPTNDAFIDSGGLDGAVPTALHPPDVNGYVPSGTTLSVYTSGGTFLYTTTV
                     GSQQTPVVSSQLGGDFNTGIIPFLEDPIYLSYTPSGSGTMSFDT"
     gene            complement(1870..2286)
                     /locus_tag="VFI55_05395"
     CDS             complement(1870..2286)
                     /locus_tag="VFI55_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073503.1"
                     /translation="MKEFLLRLGLAASLAVSGASHAYLYIHGYQHIPTIGTAFLIQAS
                     VAFALAVLIVAGGPWWLEWAAAATAGGSLVAFVLSRTVGLFGFTERGWDPSPHAAITV
                     IAEALCVLLWAGALVGSRRAQVSKLIVPEPLGVYER"
BASE COUNT          397 a          745 c          783 g          362 t
ORIGIN      
        1 ggggcatggt gacatcagcc tttcgtcgag agcgtgatgg cgttattttg tcgcggctgc
       61 gaggcaactt cgaactgggg tggggtttcg gtggattgtc gggagttcga aggcaacgcc
      121 ccccaccccg gcgggatctc cgaaatatgc tctcgggcac ggtgattgcg aaaacgtcca
      181 tttgcgccgc ccgtaacgcc taccatccgc ttgtcgctga tcgggcgacg cctcgatcgc
      241 aagggcctaa cggaggtgcg cgatgtcgtt tgtgatcact gcacccgaga tgatgaccgg
      301 cgcggcaacc gatctggcca gcctcggatc ggcgatcagc gcggccaacg cggcggcggc
      361 ggcgcgcaca accggggtgc tggcggccgg tgccgatgag gtgtcggcgg cgatcacgac
      421 gttgttttcc cagtacgcgt cggcatatca ggcgatgagc gcccaaaccg cggcgtttca
      481 cgccgagttg gtacataccc tcaacgcgag tgcgaacgtg tactcggccg ccgaggcagc
      541 caacgcatcg ccgctgcaga ccctcgagca agacgtgctg gggatgatca atgcgcccac
      601 caacacgctg atagggcatc ctctgatcgg ccacggcgcc gacggcacca ccaacgcgca
      661 aggcgcgggg acgcccggcg gccccggtgg gcttttgatc ggcagcggcg gcaacggcgg
      721 aaatagcacc gccgccggag cccccggcgg tgccggcggt ccggccgggt tgatcggccc
      781 cggcggcagt ggtgggacgg gcgggtgggg cgctcgcggc ggcgtcgggg gcaccggggg
      841 ttggctgtgg ggcaagggcg ggaccggtgg catcggtggg ccattagcgt tgggtggcac
      901 cggcggaagc gcgcggttct tcggcgtcgg cgggaccggc gggatgggtg gggaatttgg
      961 gcccggcggc gccgggggcc gcggcgggtt cctgatcggt aacggcggca tgggcgggac
     1021 cggcggagtc gtctcgggta ccggcggccc gggcggtcag gccggcatgt tgggcgcacc
     1081 agggcccgcc ggcgccgccg gcggggcgcc catcgtcccg ctgcagtcgc aggacgagcg
     1141 cctgatcgca gacgtctcag taggcggcgc accgagcgtg cccgtgatcg tcgacaccgg
     1201 atccacgggc ctgatcgttc cgccccagga tgtcaatatg caggcgctcg gagccgttac
     1261 cggacacggt agcgtcactt acggcgaacc cgggaacacc ctgaccgaaa cgtacaacac
     1321 gtacacaacg acggtgaact tcggaaatgg gatcaccacc gcgccgacca gcgttgccgt
     1381 cgtcacctcc gtaatatcaa atggcacttc atattccacg tcggaggcac ccgccatttt
     1441 gggtatcggt gtgaacgccg ggggccccac caccagtcct gtgccggctt tgccgggcgg
     1501 cctcgggcag ggtgtgctct tagacgaacc cggcggcgtc atggagttcg gcgccaaccc
     1561 gctgcccgcc tatgcctcgg tttccggagc gccggttacc accctggacg tcaagatcaa
     1621 taacgggact ctcaccccga ccaacgatgc gttcatcgat tccggtggtt tagacggagc
     1681 cgtccccaca gcccttcacc cccctgacgt caacgggtac gtgccgtcgg gaacaaccct
     1741 ttccgtctac accagcggcg ggacattttt gtacacgacg acggtcggtt cgcagcagac
     1801 gcctgtcgtg tcaagtcagc tgggaggcga cttcaatacc gggatcattc ccttcctgga
     1861 ggatccgatc tacctctcgt acactcccag cggttccggg acgatgagct tcgacacctg
     1921 agccctcctg gaaccgacca aagcacccgc ccacagcagc acgcacagcg cctcggcgat
     1981 cacggtgatc gcggcatgcg gcgaagggtc ccatccgcgc tcggtgaagc cgaagagccc
     2041 gacggtcctg gacaggacga acgccaccag cgatccgccc gccgtcgcgg ctgccgccca
     2101 ttcgagccac cagggaccgc cggcgacgat caggacagcc aacgcgaacg cgacactggc
     2161 ctggatcagg aatgccgtgc cgatcgtcgg aatgtgttgg taaccatgga tatacaggta
     2221 agcgtggctg gcgccgctga cggccaagga tgccgccaga ccgagccgga gcaagaattc
     2281 cttcaat
//
LOCUS       DASSGQ010000114         5191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_584902,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000114 DASSGQ010000000
VERSION     DASSGQ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584902"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="VFI55_05400"
     CDS             complement(<1..1124)
                     /locus_tag="VFI55_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET7073504.1"
                     /translation="MAHDTKASLKTSWYLLGPAFVAAIAYVDPGNVAANVSAGTQYGY
                     LLLWVIVGANVMAGMVQYLSAKLGLVTGRSLPAAIGTRMNRPLRLTFWAQAEIVAIAT
                     DVAEVMGGAIALRILFGLPLPVGGILTGAISLLLLTIKDRRGQLLFERVITGLLLVIA
                     VGFAASFFVATPPPDAVLGGLVPRFQGTESLLLAAAILGATVMPHAVYLHSGLALDRH
                     GHPEAGERRRWLLRVTRVDVVLAMTVAGTVNAAMLLVAAIHLRGRHETASIDGAYAAI
                     HDTLGPAIAVLFAIGLLASGLASASVGAYAGAMIMQGLLHRSIPMLVRRVVTLCPAVA
                     ILALGFDPTRALVLSQVVLSFGIPFAVLPLVRLTGNRDLMG"
     gene            complement(1218..1931)
                     /locus_tag="VFI55_05405"
     CDS             complement(1218..1931)
                     /locus_tag="VFI55_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7073505.1"
                     /translation="MSKRRVLVTGASKGIGRAVANRMAAGGHEVIGLARTAPSGFPGR
                     FHEVDLADRSATAQTLDTIVGEGAVHAVVNNVGLARFARIGSIDLDDLFTTYDMNVRT
                     AVQVVQAVLPGMLDARWGRIVNVTSLTTLGTPERTPYAASKAALETCTRIWAGELAST
                     GITVNAVSPGPIETELFRERSPAGSEREARFLASIPLHRVGTPAEIAHVIAMLLHDDA
                     GYMTGQIVRVDGGGSIGTA"
     gene            1979..2203
                     /locus_tag="VFI55_05410"
                     /pseudo
     CDS             1979..2203
                     /locus_tag="VFI55_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908715.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
     gene            complement(2226..3320)
                     /locus_tag="VFI55_05415"
                     /pseudo
     CDS             complement(2226..3320)
                     /locus_tag="VFI55_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396130.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
     gene            complement(3344..4135)
                     /locus_tag="VFI55_05420"
     CDS             complement(3344..4135)
                     /locus_tag="VFI55_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7073506.1"
                     /translation="MILDRFRLDDKVAVITGGGRGLGAAIALAFAEAGADVLIASRTQ
                     SQLDEVTEQVRAAGRRAHAVAADLAHPEATAQLAAQAVEAFGKLDIVVNNVGGTMPNT
                     LLTTSTKDLKDAFTFNVATAHALTTAAAPLMLEHSGGGSIINITSTMGRLAGRGFAAY
                     GTAKAALAHYTRLAALDLCPRIRVNAIAPGSILTSALDVVASNDALRAPMEKATPLRR
                     LGDPVDIAAAAVYLGSPAGSFLTGKTLEVDGGLTYPNLDIPVPDL"
     gene            4410..>5191
                     /gene="pstS"
                     /locus_tag="VFI55_05425"
     CDS             4410..>5191
                     /gene="pstS"
                     /locus_tag="VFI55_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET7073507.1"
                     /translation="MKLNRFGAALSVLASAALVVSGCGNDNNNASGGSATTGASSAKV
                     SCGGKNTLKASGSTAQANAMTRFVKAFEQACPGQTLNYTANGSGAGISEFTGNQTDFG
                     GSDSPLAAKEYAAAQQRCGSPAWNLPVVFGPIAITYNVKGLSSLTLDGPTAAKIFNGA
                     ITTWNDPAILAINSGVSLPAEPIHVVFRNDQSGTTDNFQKYLDAASNGAWGKGTGKTF
                     NGGVGEGAKGNDGTSAAIKATEGSITYNEWSFAKAQNLNMAKV"
BASE COUNT          938 a         1672 c         1730 g          851 t
ORIGIN      
        1 cccatcaggt cgcggttgcc ggtcagcctg accagtggaa gcaccgcgaa cgggataccg
       61 aaggacagca cgacctggga cagcaccagc gcgcgtgtcg ggtcgaaccc gagcgccagg
      121 atcgccaccg cggggcacag cgtgaccacg cggcgtacca gcatgggaat cgaccgatgg
      181 agcaatccct gcatgatcat ggcgccggcg tacgcgccca ccgacgccga cgccaggccc
      241 gacgccagca aaccgatcgc gaacagcacc gcgatcgccg gccccagcgt gtcgtggatc
      301 gcggcgtagg caccgtcgat ggacgcggtt tcgtggcggc cccgcaggtg gatcgcggcg
      361 accagcagca tcgccgcgtt caccgttccg gcaacggtca ttgccagcac gacgtcgacg
      421 cgggtgacac gtagcagcca gcgtcggcgc tcgcccgcct cagggtgtcc gtgccgatcg
      481 agggccagac ccgagtgcag atacaccgca tgtggcatca cggtggcgcc cagtatggcg
      541 gcagccagca gcaaactttc ggtgccttgg aaccgcggca ccaatccccc gagcacggca
      601 tcgggcgggg gcgtggcgac gaagaagctg gccgcgaagc cgacagcgat gaccagcagc
      661 aagccggtga tcacacgctc gaaaaggagc tgcccgcgac ggtctttgat agtcaggagc
      721 agcaacgaga tcgccccggt gaggatcccg ccgaccggca gcggcaaacc gaacaggatc
      781 cgcaatgcga tcgccccacc catgacttcc gctacgtcgg tggcgatcgc cacgatttcg
      841 gcctgcgccc agaaggtcag ccggagcggg cggttcatcc gcgtgccgat cgccgcgggc
      901 agcgaccgtc ccgtcaccag gccgagcttg gcggacaggt actgcaccat gcccgccatc
      961 acgttggcgc cgacaatcac ccacagcagc aggtagccgt attgcgtgcc ggcgctgacg
     1021 ttggccgcga cgtttccggg atcgacgtag gcgatggcag cgacaaacgc cggcccaagg
     1081 agataccaac tcgtcttgag cgaggccttg gtgtcgtgcg ccaacgtatc gcccctcgat
     1141 ccatgttccc gaataagaaa gttagggtag tcgaactgtt ccgcggcgca cctcggggcc
     1201 gtcctcaacg gtcggcgcta ggccgtcccg atgctgccgc ctccgtcgac ccggacaatc
     1261 tgccccgtca tatatccggc atcgtcgtgc agcaacatcg cgatcacatg agcgatctct
     1321 gcgggagtgc cgacccgatg cagcggaatg gacgcgagga aacgcgcttc gcgttctgat
     1381 cccgcggggc tacgttcgcg gaacaactcg gtttcgatcg gccccggtga caccgcgttc
     1441 acggtgatgc cggtcgacgc aagctcaccg gcccatatgc gcgtacacgt ttccagggcg
     1501 gcctttgaag cggcgtaggg agtgcgttcg ggggtgccga gcgtcgtcag gctcgtgacg
     1561 ttgacgatgc gaccccatct ggcgtcgagc atgccgggca gcacagcttg caccacctgc
     1621 accgcggtgc gcacgttcat gtcgtaggtc gtaaagaggt catccaggtc gatcgagccg
     1681 atccgcgcga atcgtgcgag gccgacgttg ttgaccaccg catgaacggc gccctcaccg
     1741 acgatcgtgt ccaatgtctg tgcggtagcg gagcgatcgg cgaggtcgac ttcatgaaat
     1801 cgcccgggaa agcccgacgg tgccgtgcgg gcgagcccga tgacctcatg cccgccggcg
     1861 gccatgcggt tcgcgaccgc gcgaccgatg cccttcgacg ctccggtgac cagaacccgt
     1921 cgtttcgaca tcgctttccc ctttacctcg tcacgatagc caacgcgatg cccccttagt
     1981 cagggcgttc gaataggcct gccgcggtca gacgttggac cactgtcccc acggttcggg
     2041 caccggaatt agcgaattca cgggcaatcg aaccgatttc ggtggttcgg acgcgcggct
     2101 ggaccccgaa catgcgctgc gccgcccggc aacgctgcgg ctcgcgagcg tgggacccgc
     2161 cggtgtcttc ggcccgatcg cgatcaccta cgacgtcaag ggttcattac ttgccgtcaa
     2221 ggcactcacg tgatcggacg gccgctatcc gggacgtata gcccccgacc ggtgatcagc
     2281 ggcaggtcca gggttgtcac gatgcccggc ggggcggcca ccacagcggg gatggcgttg
     2341 acgacgcgca tcgcggtggc gaccagtccg gcgtggttgt gatcgccgtg gcggctgctc
     2401 agggagaggt ctagcgcgta cgacggttca ccggtgatct cgatgcgata ggagccgccg
     2461 ggctgggccg gctgcggcca atcggggcac aggtcctcgc gcagccgggt gacatgttcc
     2521 aggacgacca ctgggtcgcc gttgaccaat ccgaacacct cgaaccgcag cgccgcggcg
     2581 gtgcccttcg cgatgtgacc cgaggcgatg tcgaagtcct cgggcgccgg ttcgcggacg
     2641 tacacctcgg cgacctcgtc cagtgaaatg cccaggcccg ctgccaattg ccgcaccacc
     2701 gaaccccacg ccaagctcag cacaccggtc tgcagcagca tggggatctc gtcgaggggc
     2761 ttgccgaatc ccatcacgtc gaacatgacc acggcgctgt cgtaggtggc gtagtcgacg
     2821 atctccatgc agcgaacctg ctggatgctc tgacaggtgc cggccaatgc cagcggcagc
     2881 aggtcattgg cgaagcccgg gtcgatgccg ttgacgtaga gactcgaatt cccttcttgt
     2941 gcggcgtctt ccagcggtgc tatgaggttt tcggggatca cttcccacgg gtactgcaga
     3001 aagaccgggc cgctgccgac aacattgact ccggcggcca ggacgcgccg gtagtcctct
     3061 agggcctcgg gcaggcggtt gtcggccatc gcgttgtaga ccgcgcagcg cggcccggtg
     3121 gcgagcacgg cctccaggtc ggtgctggcc agcacgccgg tcgagtccgc aagtccggca
     3181 agctctgcgg cgtccttgcc ggctttggcg tcggacgact accacacacc ggtgagttcg
     3241 aattcggggt tgatgatcag tgcccgcaac gcgtggacac cgacgttgcc ggtgcccaat
     3301 tgaacgacgg gaatggccat gacgggctcc ttagcggtcg cgtttacagg tccgggacgg
     3361 ggatgtcgag attggggtag gtgaggccgc cgtcgacctc cagggtcttg ccggtgagga
     3421 agctcccggc gggggacccc aaatagactg ccgcagccgc gatgtcgacc ggatcaccga
     3481 ggcggcgcag cggcgtcgcc ttttccatcg gcgcccgcag cgcgtcgttg gaggccacca
     3541 cgtccagtgc ggaggtgagg atcgaccccg gtgcgatcgc attgacccga atgcgcgggc
     3601 acaggtccag tgcggccaac cgggtgtagt gggccagcgc ggctttggcg gtgccatagg
     3661 cggcgaaacc gcgtccggcc agcctgccca tggtcgaggt gatgttgatg atgctgccgc
     3721 cgccggagtg ttcgagcatc agtggcgccg ctgccgtggt gagcgcgtgg gcggtggcga
     3781 cgttgaaggt gaacgcgtcc ttgaggtctt tggtcgacgt ggtcagcagc gtgttgggca
     3841 tggtcccgcc gacgttgttg acgacgatgt ctagttttcc gaaagcctcg acggcctggg
     3901 cggccagctg cgcggttgcc tcgggatggg ccaggtcggc ggcgacggcg tgggcccgtc
     3961 ggcctgcggc gcggacctgc tcggtgacct cgtccagttg tgattgcgtg cgcgacgcaa
     4021 taagaacgtc ggcacctgcc tcggcgaaag ccaacgcgat ggccgcaccc aggccgcgac
     4081 caccaccggt gatcacggcg actttgtcgt ccagacggaa cctgtcaagg atcatcgcgg
     4141 cctttctatg cgagtcgtcg gccgaaacgg tagcaaactt cgctgtctcg gctacggcgt
     4201 ttctgaaaca ggttctagtt tgccatacgg gcgcggcgac gcgacgcggc ccgtcgtcga
     4261 ttgtgcgccg gccgcgcacg gtgacgactg ccggaaaaaa ttcgccgaac gttaaccttg
     4321 ttactgttag cttcatggct gcgaggaaac ggctggttgg cgggccgcag ccattggtag
     4381 cggcgatgtc cttaatgtta ggaactgaat tgaagctcaa ccgatttggc gccgcgctaa
     4441 gtgtcctggc tagcgccgcg ctggtggtat ccgggtgtgg caacgacaac aacaacgcca
     4501 gtggcggaag tgcgaccacg ggcgcatcgt cggcaaaggt gagttgcggc ggaaagaaca
     4561 cgctgaaggc cagtgggtcg accgcccagg ccaacgcgat gacccgcttc gtcaaggcct
     4621 tcgaacaggc ctgcccgggt cagaccttga actacacagc aaatggttcg ggcgccggaa
     4681 tcagcgaatt caccggcaat caaaccgatt tcggtgggtc cgactcgccg ctggcagcga
     4741 aggagtacgc cgccgcccaa cagcgctgcg gttcgccggc gtggaacctg ccggtggtgt
     4801 tcggcccgat cgcgatcacc tacaacgtca agggcctcag ttcgttgact ctcgacggtc
     4861 ccaccgcggc gaagatcttc aacggcgcca ttaccacctg gaacgacccc gcgatcctgg
     4921 ccatcaactc gggcgtcagc ctgccggccg agccgattca cgtggtgttc cgtaacgacc
     4981 aatccgggac cacggataac ttccagaaat acctcgacgc ggcgtccaac ggtgcgtggg
     5041 gcaagggcac cggcaagacg ttcaatggcg gcgtcggcga gggcgccaag ggcaacgacg
     5101 ggacctcggc ggccatcaag gccaccgaag gatcgatcac gtacaacgaa tggtcgttcg
     5161 ccaaggcgca aaacctgaac atggccaagg t
//
LOCUS       DASSGQ010000115        16853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_68332, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000115 DASSGQ010000000
VERSION     DASSGQ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 16853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16853
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68332"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VFI55_05430"
     CDS             complement(<1..597)
                     /locus_tag="VFI55_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7073508.1"
                     /translation="MSGHGTPLRIGILGAARIAPMALIKPAKDNSDVMVAAVAARDAS
                     RAKAFAAAHDIPRVHESYDALIADPNLDAVYNPLPNGLHGKWTRAAVAAGKHVLCEKP
                     FTANAAEAREIAAFAANSDRVVMEAFHYRYHPLTLRVEEIIASGELGKLTRVEAALCF
                     PLPKFSDIRYNYSLAGGATMDAGCYAVHMVRTFGGATPE"
     gene            complement(876..2480)
                     /locus_tag="VFI55_05435"
                     /pseudo
     CDS             complement(876..2480)
                     /locus_tag="VFI55_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725184.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            2611..3630
                     /locus_tag="VFI55_05440"
     CDS             2611..3630
                     /locus_tag="VFI55_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET7073509.1"
                     /translation="MLAQFGMSIPLIAAPMSGGPTTPAMVLAATRAGGLGLLAAGYKT
                     VDAIEAEIKKVRGEGIPFGVNVFAPNPLPVDPDSYRTYAATIQRDADQFGLTLPAEPI
                     EDNDRFDEKIALLLDDPVPMVSFTFGIPPRNVIAALQQANSVVVQTVTTPDEAAQAYE
                     AGVDMLAVQAAAAGGHSGTLSPRKPLTPVPIVDLVKRIVATVPLPVMAAGGLATPAAV
                     AEAVRAGAAAAVVGTVLLRAAESGASATHQAALIDPAFTETVLTRAFTGRPARGLRNA
                     FIDAHEGEAPLGYPAIHYLTSPLRKAAAAAGKPNYVHLWAGTGYRHATAEPASDILRR
                     LTSEL"
     gene            complement(3729..4997)
                     /locus_tag="VFI55_05445"
     CDS             complement(3729..4997)
                     /locus_tag="VFI55_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7073510.1"
                     /translation="MKSIDELAANLNFTTAKTGGQRSVTFLPDPPRAPRRYTVISVDD
                     HIVEPPDTFAGRLPRKFADRAPKVVETESGGQTWVYDDRELPNVGFNAVVGRPVAEYG
                     FEPVRFDEMRRGAWDIHERVNDMDLNGIYASLNFPSFLPGFAGQRLQQVTKDRDLALA
                     SVRAWNDWHLEVWAGSYPDRIIPCQLPWLLDPELGAKMIRENAERGFHAVTFSENPAM
                     LGLPSIHSGHWDAMMAACAETGTVVNLHIGSSGSSPSTTDDAPPDVQGVLFFAYAISA
                     AVDWLYSGVPSRFPDLKICLSEGGIGWVAGLLDRLDHMLSYHAMYGTWAALGESLTPA
                     EVFTRNFWFCAVEDKSSFVQRDRIGVDNIMLEADYPHCDSTWPHTQQTIHEQIGELPQ
                     HVIRKLSWENASRLYRHPVPVDVQRNPEAF"
     gene            complement(5106..5879)
                     /locus_tag="VFI55_05450"
     CDS             complement(5106..5879)
                     /locus_tag="VFI55_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET7073511.1"
                     /translation="MNARNVRRGGVEVWAPSVVPPIGVELSDEQALAVAFRHLAAIGF
                     AENMAGHITWQLDGQTDMFVNPWGLWWQELTASDICVVDSDARVIRGRWDVTPAIHIH
                     TELHRVREDARVVIHNHPYYVCVLAALGRLPELVHQTGSLYLDDLCLVDTYDGEVDSP
                     ARAADLATRIGGANLTILANHGVLATGRNLPEAVYRAASIERVCKLAYDVMLTGKEPL
                     PMSWSDMAGMQRSLIERAADVYWAGAARMTIKADPDVLN"
     gene            complement(5866..7488)
                     /locus_tag="VFI55_05455"
     CDS             complement(5866..7488)
                     /locus_tag="VFI55_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HET7073512.1"
                     /translation="MGAGGLETVGSPGFTAEDPARFRAAGWWSDSTLSDAVRRNAERS
                     PSHPAYIDHPGVSLSWYEFDVAANNLAEQLAGVGVVRGDRVAVWHSDSAAIHVLFVAL
                     ERCGAVVVGIGARAGTREVAMVLCASQPKILISDHDHSSAAARVVDDSLVFALTLTAD
                     GGPLRLNVGAEPRTLGIESELGPDDVFLINSTSGTTGLPKCVVHTQNRWFYFHQKAVA
                     NGLLTADDIFLPAIPTPFGFGIWTSHTTPIYLGATAVILTRFTVNTACEAIARHQVSV
                     LCCVSTQLMMLMADRARHDHDLSSLRVVFAGGEALPYRPAAEFEELTGAKILQFYGSN
                     ETGMLSATTVDDPRERRLRTGGRIVPEMAVRLFDGDNDVTETGRGQPACRGPATSLGY
                     LDGTDHDKLFTPDGWMRMGDICEIDADGYLTVTGRTSDFILRGGKNISATQVENAVTT
                     HPAIAVAAVVAMPDPVLGEKVCLYAELADSQAIDLPELIEHLLALGVSKELLPERLIV
                     VDELPRSPGGKIAKGQLREDIQFRMEADHERS"
     gene            8005..8568
                     /locus_tag="VFI55_05460"
     CDS             8005..8568
                     /locus_tag="VFI55_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020728891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073513.1"
                     /translation="MKTTRISGLWPGLLSGAAAGAAGTSALNVITYLDIAVRGRPTSS
                     TPERTVEAMARLVGLTVPGTGDILANRLLGLGALTGYAAGIGMGLILGLVWALGWRPG
                     ILVATLVATALALIGTNGPMTVLGVTDPRTWGLVGWISDVIPHFGYGVVTALVLHYLY
                     PGNPGNPRIPSKPPSAGAREPEATESV"
     gene            8661..9023
                     /locus_tag="VFI55_05465"
     CDS             8661..9023
                     /locus_tag="VFI55_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073514.1"
                     /translation="MKTKIVVVLSGVLVALLLLSRYFALSRVGVPVGWMLYLGLPIAA
                     GGVLLALRIANLGAGWANKIDHIERNGAKRSRPSLALSRSTAVVSERLEELQVLHDTG
                     AISGAEYRARRVQIIAGL"
     gene            9058..9972
                     /locus_tag="VFI55_05470"
     CDS             9058..9972
                     /locus_tag="VFI55_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7073515.1"
                     /translation="MACGKTSRAPCRGYCRFVLIGFLLALGCSVCYGTATVLQAMATR
                     SVETNSSSGVNAVLLLRAIQQWRYLAGIGLDGLGFLLQVMALRLVPIYVVAAALATSI
                     AVTGIVSAWALSARLSPIEWTAVAVVCSSLVALALAAGPAHFRHAPPALGWALLGVVA
                     AIVVVGAAAGRLADRPRGLALGLGAGTGFGVVEIGVRLLDVVNPAKAAFYANPALYAA
                     AGGGAAGFLLLTSALQRGSVTTTVAAMVVGETIAPALVGVIWLGDTARDGLGWVVVVG
                     FAIAVAGTLVLARFGEAPEPAEAGQPAG"
     gene            10138..10707
                     /locus_tag="VFI55_05475"
     CDS             10138..10707
                     /locus_tag="VFI55_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase"
                     /protein_id="HET7073516.1"
                     /translation="MNGTSDFGTFGRFVETPVDEMSPEMKEAYEFTRQLRGLVPGPHK
                     IWLANPTLSKTVVPTGAYFQRQSTLTKAEIEIATNIVNGRWRSAYANYEHEIVGERDG
                     HLDPRRVEALIAGLPTSFDDPRQQVVYELATTLVAPRVVPLGLYRRAKELLGDAGIID
                     IAVLLGWFTMVCMTLGAFDVPANAEGLDQ"
     gene            10753..11760
                     /locus_tag="VFI55_05480"
     CDS             10753..11760
                     /locus_tag="VFI55_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073517.1"
                     /translation="MALPPLPIVRLTPVAFFPQKYFLENLAVRADESVLITAVLQNEL
                     WWVPPAKSDAVVQPVLVHAFDHPVTGIAEVAPDVFVVCLTEAYTTHESHLVRIDFNGW
                     TPGTPVWPLVIFTFDDRVRGLNGSCRLGPNVLAIADCFAGLIWRVNLADDARSATARV
                     WLSDDTMAFDPDSGVPPPPQPGVNGVRYGVETGYLYYTSTAQKVFMRVAVDASTLDPI
                     AQPQFVAAIDNADDFCLDEKAGFAYVTRHRANTIDRVPLASGHGGEVRHIAGDPFDAT
                     LIGPSSVAWGRGRSDHGRIAYVTTDGGTTAPPHGIVRQAALLRAEFDYPGQPRPAARE
                     L"
     gene            complement(12124..12345)
                     /locus_tag="VFI55_05485"
     CDS             complement(12124..12345)
                     /locus_tag="VFI55_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073518.1"
                     /translation="MEDPAQGRLITALPWARREGGRLGPFANRLIGSGAHPIRQQQQI
                     MLTYPERQRVRQLPQLDGTIAQLLAEACL"
     gene            complement(12318..15032)
                     /locus_tag="VFI55_05490"
     CDS             complement(12318..15032)
                     /locus_tag="VFI55_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET7073519.1"
                     /translation="MVLVSPPAPPVLTVRYEGSERTFAAGTDVVIGRDLRADVRVAHP
                     LISRTHLIVRFDQGRWMAIDNGSLNGLYVNNRRVPVVDIQDGLRVNIGNPDGPALTFE
                     VGRHQGSAGRPPLTTSIPILNPAGGPGDRVSQSQPQVAAPWPGQPQHPPPVPPWQPPP
                     PLSGPQHPPPPSAPQVSHPPQVSHPPSGPQPIYPPGPPAQYPTAGHPAGMPSGPQQAP
                     KIYRAQPMKVPPAPAGEAETGRVGDSTNIATSMMKILRPGRTAMESAPGAIKIGRAND
                     NDIVIPEVLASRHHATLIPTPYGTEIHDNRSINGTFVNGARVESALLHDGDVVTIGNI
                     DLVFAGGTLARRDETATATRTGGLDVRGVTWTIENNKTLLEDISLGAQPGTLTAVIGP
                     SGAGKSTFARLVAGYTHPTTGTVAFEGHNVHGEYASLRSRIGMVPQDDVVHGQLTVQQ
                     ALMYAAELRLPPDTTKADREQVVARVLEELEMSQHLHTRVDKLSGGQRKRASVALELL
                     TGPSLLILDEPTSGLDPALDRQVMTMLRQLADAGRVVLVVTHSLTYLDICDQVLLLAP
                     GGKTAFCGPPSQIGPAMGTTNWADIFSTVANDPDGAKARYLARTGPPPPPPPAEKPAE
                     LGDPSHTSLFRQFSTIARRQIRLIVSDRGYFIFLTVLPFIMGSLSMSVPGDVGFGIPN
                     PLGDAPNEPGQILVLLNVGAVFMGTALTIRDLIGERAIFLREQAVGLSTSAYLLAKVC
                     VYTVFAVVQSAIVSAITIIGKGGPTQGAVALGKPGLELFVDVAATCVASAMLGLALSA
                     VAKSNEQIMPLLVVAVMSQLVFSGGMIPVTGRIPLDQMSWATPARWGFATSASTVDLT
                     KLVPGPLTPKDSHWRHDAGAWWFDMGMLGVISMVYLGFVRWKIRLKGG"
     gene            15252..16097
                     /locus_tag="VFI55_05495"
     CDS             15252..16097
                     /locus_tag="VFI55_05495"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7073520.1"
                     /translation="MDEAMCAAPLLADRVAVVTGGGGGIGAATARLFARHGARVVIAD
                     IDTDLAKRTTGEITASGGSALAIVTDVRDADQVAGLVRVVLDRYGRADVLVNNVGHWL
                     RHPGNFVDTDPQLWDDLYRINLHHVFSVTHAFLPTMINQHCGAIINVSSVEGLRGYPE
                     DPVYAAFKAGVIHFTRSLAVQVGRDGVRVNAIGPDVTESLQVPYSRWLSAQEQTLWPQ
                     WVPVGRMGLPEDQARVILFLASDLSAFVTGHTIPTDGGTRAAGGWFRSSRRPDREWTN
                     RPVAP"
     gene            complement(16273..>16853)
                     /locus_tag="VFI55_05500"
     CDS             complement(16273..>16853)
                     /locus_tag="VFI55_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HET7073521.1"
                     /translation="VEQLLNAQTAAVTTAQRFGLLGGFPFAPIAGMDPLPPARDESRK
                     LADAASRVEILVGYTRNDADAFVAMDSRGQRLQRLGPPGRAITRLLGDVITRRIFGQP
                     ALKLAKSWTSNGGTAATFRVEWSPPGAPLRACHCIDLPLLFGSPECWADAPMLGPEPN
                     PIDTELARKARGYWAAFAHGGTVALGSSSLRI"
BASE COUNT         2873 a         5430 c         5648 g         2902 t
ORIGIN      
        1 ttccggcgtg gcaccaccga acgtgcgaac catgtggact gcgtagcaac cggcatccat
       61 cgtcgcgcca ccggcgagcg aatagttgta gcggatatcg gaaaacttgg gcagcgggaa
      121 gcacaaggcg gcctccaccc gcgtgagctt gccgagttcg cccgaggcga tgatctcctc
      181 gacgcgcaaa gtgagcggat ggtagcgata gtggaatgcc tccatcacca cgcgatcaga
      241 gttcgcggcg aatgcggcga tctcgcgggc ctcggccgca ttcgcggtga acggcttttc
      301 gcacagcacg tgcttaccgg cggcgacggc ggcccgggtc cacttgccgt gcaggccgtt
      361 cggcagcggg ttgtatacgg cgtccaggtt cgggtcggcg atcagcgcgt cgtagctttc
      421 gtgcacacga gggatgtcgt gtgcagccgc aaaggctttc gcgcgcgacg cgtcgcgcgc
      481 ggcgacggcg gcaaccatga cgtcggagtt gtccttggcc ggtttgatca gcgccatggg
      541 cgcgatgcgg gccgcaccca ggatgccgat gcgcagcgga gtcccgtgtc cagacacgca
      601 cccgacgcta gcacttggcg cgggtcgcca ggagaagcga aagtagcccc caacgggcct
      661 tagccgcgta gatcgtgatc acatctgtca cacgcgggtg tcccagagac ggcgcacttt
      721 ttgcccacct ccgagcgact gatccacgac gggccacgca ttgtcgctcg caggcgggca
      781 cgttcaataa gtcaacgcgc ggacctgccg gcagagtcgt gtggcggatg agaccccggg
      841 gctcgaagaa cccggcgagc ttagggcgga gaatgtcagt cggcttcgaa cgccgagaca
      901 gcgtcgagga tcacgccgtt ctcctcgacg tagcggtccc acagcgtcag cagctcggcc
      961 agcttctcgg gccgcgccgc ggccagatcg tcaatttcgc cggggtcgcg ggagaggtcg
     1021 tatagctgcc agcggcccgg accgtatggc ttcggcaggt agagcgcctt ccagtcgccc
     1081 tgacgaattg cccggcggcc gaacagctcc cagcccgtgc aggtttcagc gtcgtgcacc
     1141 gcgtcgctgg cgccggacag atacggcacc agcgagcgac cgcgtagcgg ttcgacttcg
     1201 cggccgcggt aggtggtgcc gggatgtgcg gcgccggcca gttccagcac cgtcggcacg
     1261 atgtccatga ccgtcgagaa cgcggtgccg atttccccct gccgcgcaaa tcccggccaa
     1321 gtgacaaagc ccaccacccg gatgccgcct tcggtggtga acgccttgtg caggcgcgac
     1381 ggcgccgttg ccgcttgcgc ccagcgtggg ccttaccaga tgaacgagtt ggggcggcca
     1441 aggttgtcga ggctgttgtc gcagtatttt gcgatctgcg cggcgatctg ggcgccacga
     1501 agcggcatcg cctcgacgat tgcgccctcc gcgccgttgt cggacaagaa gatcaccacg
     1561 gtgtcatcaa gctcgccgct gtcggccagg tagtcgatca cccggccgat attccagtcc
     1621 attcggtcca ccattccggc gtagacctcc atgctgcggg ccgacagcgc gcgctcctcg
     1681 gtcgtcatgt cggcccactc cggagcgccg ctcgccacga ccggatgcgc cacgacatcg
     1741 ggcgggcaca ggccgagccg tttgagcgcc gtcaagcgcg cctcgcgcaa cgcgtccggt
     1801 ccggcggcgt agcggccgtg ataggcggcg atggattcgt cgggcgcttg cagcggccag
     1861 tgcggcgcct gaaagggcag atacgcgaag aacggccggt ctttgtcgtg cggatccaca
     1921 gaacgttcgt ggaggtagcg cagcagggtg tcagtgtaga aatcggaaga gtagaagtcg
     1981 tcgccgacgg tgacgaactg gtcatcctcg gtgtaaagca ttggcaacgg cgggaaaccg
     2041 ccgcccgcgg cgctgtaatg gctggcaccg gcgggtaaca aggcgaacga gcggtcgaat
     2101 ccgcgagccc acggcgacct ctcgatggtg taccccagat gccatttgcc cgacatcagc
     2161 gtcagatatc ccgcgtcgcg caacaactcg ggcaacgcca ccacccggtc gttgagatag
     2221 ccctcatagc cgggcgcacc ccggaatcct ggattcgcga cctcgagcat cgtgccgatg
     2281 ccggcaatgt ggtggtcgat tccggtcagc agcatcgccc gggtgggtga acaggccgga
     2341 gcggaatgga aatcagtgaa ccggatgccc gcataggcca gctgatccag attgggcgta
     2401 ttgatctcgc cgccgaacgc gccgatatcg gagaacccga ggtcgtccgc gacgatcaac
     2461 agaaagttgg ggcgcttcac gctgacgcat cctttcaggt agggcgccga gatggaaccg
     2521 cacaccagtg aaccgcaccg gcagcatcgc cgatatcgtc aagacagcac ttgatccccc
     2581 gcgccggaca caccgaagaa ggggcttgaa atgttggcac agtttgggat gtcaatcccg
     2641 ttgatcgctg ccccgatgtc gggcggtccg actaccccgg cgatggtgtt ggcagcgacg
     2701 cgggccggtg gcctgggctt gctggcggcc ggctacaaga ccgtggatgc catcgaagct
     2761 gagatcaaga aggtgcgtgg cgagggaatc ccattcgggg tcaacgtgtt tgcgccgaat
     2821 ccgctgcccg tcgacccgga cagctaccga acctacgccg caacgatcca gagggacgct
     2881 gaccagttcg gactcacgtt gcctgccgag ccaatcgagg acaacgacag gttcgacgag
     2941 aagatagccc tgttgctcga cgacccggtg ccgatggtgt cgttcacgtt cggaataccg
     3001 ccccgtaatg tgatcgccgc cctccagcag gccaatagcg ttgtcgttca aacggtgaca
     3061 acgcccgacg aggcggcgca agcctacgag gccggtgtcg acatgctcgc cgtgcaggca
     3121 gcagccgccg gcgggcattc cgggacactc tcgccgcgta aaccgttgac gccggttccg
     3181 atcgtggacc ttgtcaagcg gatcgtggcg acggtaccgc taccggtgat ggccgcgggt
     3241 ggactggcca ctcccgccgc ggtggccgag gctgtccgcg ccggcgctgc agcggccgtc
     3301 gtcggaacgg ttctgctacg tgccgccgaa agcggcgcat cagccaccca ccaggcggca
     3361 ctcatcgacc ccgctttcac cgagacggta ctgacgcggg ccttcaccgg ccgccctgcg
     3421 cggggcctgc gcaatgcgtt catcgacgcg catgaagggg aagcaccctt gggatatccc
     3481 gcgattcact acctcaccag cccgttgcgc aaagccgccg ccgcggccgg caagcccaac
     3541 tatgtccatc tatgggccgg cacgggatac cgacacgcca cagccgaacc cgcgtccgac
     3601 atcctgcggc gactgacttc cgagctctaa ttgattggcg gaccccttac cgacgcatcc
     3661 cctcggtgcc ggtgcgcccg ctctggcggc tgctcagtac cacaccgacc ccgcagctcg
     3721 gcgggacgtc aaaacgcctc gggattccgc tgtacatcga ccggcaccgg gtggcggtac
     3781 aggcgcgacg cgttctccca gctcaacttg cggatcacgt gctgcggcag ctccccgatt
     3841 tgttcgtgga tcgtttgctg ggtgtgcggc caggtggagt cgcagtgtgg atagtcggcc
     3901 tccagcatga tgttgtccac gccgattcgg tcgcgctgta cgaacgacga cttgtcctcc
     3961 actgcgcaga accagaagtt gcgggtgaaa acctctgcgg gagtcaaact ttcaccaagc
     4021 gccgcccaag taccgtacat cgcgtgatag ctgagcatgt ggtcgaggcg atccaacagg
     4081 cccgcgaccc agccgattcc cccttcggac aaacagattt tcagatcggg aaacctgctg
     4141 ggcaccccgg agtacagcca gtcgaccgcc gcggaaatcg cataggcgaa gaacagcaca
     4201 ccctgtacgt ccgggggtgc atcgtcggtg gtggacggcg acgaccccga cgatccgata
     4261 tgcaggttga cgacggtgcc cgtctcggcg cacgcggcca tcatcgcgtc ccagtgaccg
     4321 gagtggatgc ttggcaatcc gagcatcgcc gggttctcgc tgaacgtgac ggcgtgaaag
     4381 ccgcgctccg cgttctcacg gatcatcttg gcgcccagct cggggtccag cagccacggc
     4441 aattggcagg gaatgatccg gtcgggatat gaccccgccc aaacctcgag atgccagtcg
     4501 ttccatgcgc gcacggaggc cagcgccagg tcgcgatcct tggtgacctg ctgcagccgc
     4561 tggccggcga agcccggcag gaaagagggg aagttcagcg aggcgtagat gccattcagg
     4621 tccatgtcgt tgacgcgctc atggatgtcc catgcgcccc tgcgcatttc gtcgaatcga
     4681 accggttcga agccatactc cgccaccggc cgcccgacca cggcgttgaa cccgacgttg
     4741 ggcagttccc ggtcgtcgta gacccacgtc tgtcccccgc tctcggtctc cacgactttg
     4801 ggggcgcggt cggcgaactt tctcggcaga cgcccggcga aggtgtcggg cggttcgacg
     4861 atgtggtcgt cgaccgagat caccgtgtag cggcgtggcg cccggggcgg gtccggcagg
     4921 aatgtgaccg agcgctgccc acccgtcttt gccgtggtga aattcaggtt tgctgccagc
     4981 tcgtcgatcg atttcattcg tcaatctcct tgcgggacag ggtcgcgcgc cgcatcgaat
     5041 gtgcagtggc ggtgaagccg gtcggcgtgt cgccgcactg ggtgcacgct gaagaccagc
     5101 acggctcagt tgagtacatc gggatcagcc ttgatcgtca tccgcgccgc tcccgcccag
     5161 tacacgtcgg ccgcccgttc gatgagcgat cgctgcatgc ccgccatgtc cgaccagctc
     5221 atcggaagcg gttccttgcc ggtgagcatg acgtcgtacg ccagcttgca cacccgttcg
     5281 atcgatgccg cccggtagac ggcttcgggc agattgcggc cggttgcgag gacgccgtgg
     5341 ttggccagaa tcgtgaggtt ggcgcctccg atgcgggtgg cgagatcggc tgcgcgagcg
     5401 ggactgtcga cttcgccgtc gtaggtgtcg accagacaca agtcgtcgag gtaaagcgaa
     5461 ccggtttggt ggacgagttc gggcaacctg cccaacgcgg cgagcacgca tacgtaatag
     5521 gggtgattgt ggatgacgac gcgggcgtcc tcgcggaccc ggtgcagttc cgtgtggatg
     5581 tggatcgccg gcgtgacatc ccagcggccg cggatcaccc gcgcgtcgct atccaccacg
     5641 cagatgtctg acgcggtgag ctcctgccac cacagccccc acggattgac gaacatgtcc
     5701 gtttgaccgt cgagctgcca ggtgatgtgc ccggccatgt tctcggcgaa tccgatggcg
     5761 gcaaggtggc ggaaggccac ggcgagcgcc tgctcgtcgg acagctcgac gccgatgggg
     5821 ggcaccaccg acggcgccca gacctccacg ccacctcgtc gcacgttacg agcgttcatg
     5881 atcggcctcc atcctgaatt gaatatcctc gcggagctgg cctttcgcga tcttccctcc
     5941 aggcgatcgg ggaagttcat cgaccacgat gagtcgttcg ggcagcagct ccttggacac
     6001 ccccagcgcc aacagatgtt cgatgagctc gggcaggtcg atggcctgcg aatcagccaa
     6061 ttcggcgtag agacaaacct tttcgccgag caccggatcg ggcatcgcga ccactgccgc
     6121 caccgcgatc gcgggatggg tcgtgacggc gttctcgacc tgcgttgcgc tgatgttctt
     6181 cccgccacgc agaatgaaat ctgaggttcg gccggtgacg gtcagatagc cgtccgcgtc
     6241 gatctcgcag atgtcgccca tgcgcatcca cccatcgggg gtgaacaact tgtcgtggtc
     6301 ggtcccgtcg agatagccga ggctggtggc gggccctcgg caggcgggct ggcctcgccc
     6361 tgtctcggtg acgtcgttgt ctccgtcgaa aagtcggacg gccatttccg ggacgatcct
     6421 gccgccggtg cggaggcggc gttcgcgcgg atcgtcgacg gtcgtcgcac tcagcatccc
     6481 ggtctcattc gaaccgtaga actgcagaat cttcgcgccg gtgagctcct cgaactccgc
     6541 cgcgggccgg tacggcaacg cctcaccgcc ggcgaaaacg acacgcagcg aactcaggtc
     6601 gtgatcgtgc cgagcgcggt ctgccattag catcatcaat tgcgtactga cacaacacaa
     6661 cacgctgacc tgatggcgtg cgatcgcctc gcaggccgta ttcacggtga atcgcgtcag
     6721 gatcactgcg gtggcaccga gatagatcgg agtggtgtgg ctggtccata ttccgaagcc
     6781 gaaaggtgtt ggtatggccg gcaagaatat gtcgtccgcc gtcagcaacc cgttggccac
     6841 agccttttgg tgaaagtaga accaacggtt ctgcgtgtgc acgacacatt tggggaggcc
     6901 cgtggtcccg gaggtggagt tgataaggaa gacgtcgtcg gggccgagtt cggattcgat
     6961 ccccaacgtt ctgggctcag cgccgacgtt caaccgcaac gggccgccat cggcggtcaa
     7021 cgtcaacgcg aacaccagag aatcgtcgac cacccgcgcc gcggcgctgc tgtggtcgtg
     7081 atcgctgatc aggatcttcg gctgcgaagc gcacagcacc atggcgacct cgcgggttcc
     7141 ggcacgcgcg ccgatgccaa cgacgacggc accgcaacgt tcgagtgcga cgaacagcac
     7201 gtggatcgca gcggagtcgc tgtgccacac cgcaacccgg tcaccgcgga ccaccccgac
     7261 tccggcgagc tgctccgcca agttgtttgc cgcgacgtcg aattcatacc agctcagcga
     7321 gacgccggga tggtcgatat acgcaggatg gctcggtgag cgttcggcgt tgcgacgtac
     7381 cgcatccgac agcgtcgaat cagaccacca gccggcggca cgaaaccggg ccgggtcctc
     7441 agcggtgaag cccggcgagc ccacggtttc cagcccgccg gcgcccatta ccaaatgata
     7501 ggaaaatcgc gcgtcgtgtc gtcagagaag tccacgcgct acgtgcgggg aagcggctac
     7561 ggatcctgcg gagcaggagg attgtgctga ttggtgcctg tattccccgg acgggggtgc
     7621 ggggttggac acgtgtggtg cgggatacag ccgcagttga ggccgataat gcaggcgccc
     7681 gcggccggct gcaccggtag ggcaccgccc ccgttcccgc cggtgatggg ttccggcgac
     7741 gggggtgctg gcacgacgct ggatgaaggg ctcgttgcgg tggtcagcgc ctcgccggcg
     7801 ccgacaacac cgaatccgat cgcaacaacc agagcggcgc caccgcagac cgcggccgcc
     7861 gtcctgcgat tgcgggtagt cacggctccc aactgtagtt tctcttcgcg agatgcgcag
     7921 aacgccacgg cccgggtctg aaagtcatag acaatgcgtc agtcgggccc gacgggagtc
     7981 gggcggcacg ggaggtggtg acagatgaaa accacgcgaa tctccgggct ttggccggga
     8041 ctgctgagtg gcgcagccgc cggggccgcg ggcaccagcg ccctcaacgt catcacctat
     8101 ctggacatcg ccgtacgcgg ccgaccgacg agctcaacgc ccgagagaac cgtggaagcg
     8161 atggcgaggc ttgttggcct cacggtgccg ggcaccggtg acatcctggc taaccggctg
     8221 ttgggcctcg gagccctcac cggctatgcc gcgggcatcg ggatgggtct gattctcggg
     8281 ctggtctggg cgctcggttg gcgcccgggc atactcgtcg ccacgctggt cgcgaccgca
     8341 ctcgccctga tcggtaccaa cggaccgatg accgtactcg gggtgaccga cccgcggacc
     8401 tggggcctgg tggggtggat cagcgatgtg atcccccact tcggatacgg ggtggtcacg
     8461 gccctcgtgc tgcattacct ataccccgga aaccccggaa acccgcgaat tccctcgaag
     8521 ccgccctcgg cgggggcgcg agagcccgag gctaccgagt cggtttgatc cccggttgtt
     8581 gggatcggta gcgacccatg cggcttgcac ttgccagatc accgccgcaa atgcggcgaa
     8641 cggcaaggag gaatccacga atgaaaacca agatcgtcgt cgtgttgtcc ggagtgctgg
     8701 tcgctttgtt gctgctgtcg cgctattttg cgctgagccg ggtcggcgta ccagtggggt
     8761 ggatgctcta cctcgggcta ccgatcgccg cggggggcgt gctgctggcg ctgcgcatcg
     8821 ccaacctggg cgcgggctgg gccaacaaga tcgaccacat agagcgcaac ggcgcaaagc
     8881 ggtcacgccc gtcgctggcg ctctcgcggt cgactgccgt ggtgtccgag cgcctcgagg
     8941 agctccaggt gttgcacgac accggtgcga tctctggcgc ggagtaccgg gcccggcgcg
     9001 tgcaaatcat cgccgggctg tgagccgaac cgccgacggc ggtgggccct gacccgcatg
     9061 gcttgcggga aaacgtcacg cgcgccgtgt cgcggctatt gtcggttcgt gctgatcggt
     9121 tttctactgg ctctgggctg ctcggtgtgt tacggcacgg caacggtatt gcaggcgatg
     9181 gcgacgcgat cggtggaaac caacagcagc tcaggtgtta acgcggtgct gctgctgcgc
     9241 gcaatccagc aatggcgcta tctcgccggc atcggccttg acggcctcgg attcctgttg
     9301 caggtaatgg cattgcggtt ggtgccgatt tacgttgtcg cggcggcgct ggccacctca
     9361 atcgccgtca ccggcatcgt ctccgcgtgg gcgttgtcgg ctcgcctgtc gcccatcgag
     9421 tggacggccg tggccgtggt ctgttcgagc cttgttgcgc tcgctcttgc ggcagggcct
     9481 gcgcacttcc ggcatgcacc accggcgctc ggctgggcac tgctgggcgt ggtggcggcg
     9541 atagtcgtcg tcggcgcggc tgccggtcga ttggccgatc gcccacgggg gctcgctctg
     9601 gggctcggcg ccggaaccgg cttcggcgtc gtggagatcg gggtccgcct gctcgacgtc
     9661 gtcaaccccg ccaaggccgc gttctacgcc aaccccgcct tgtacgcggc ggccggtggc
     9721 ggggccgcgg gatttctctt gctcacatcc gcactgcaac gtgggtcggt gaccacaacg
     9781 gtggccgcca tggttgtggg tgaaacaatc gccccggcac ttgtcggggt gatctggctg
     9841 ggtgacaccg cccgggacgg cctcggctgg gtggttgtcg tgggattcgc gatagctgtg
     9901 gccggaacac tcgtgctggc gcggttcggc gaagcgcccg agcccgccga ggccggccag
     9961 cccgccggat aagtcacgct gcggccaagc cgcgcaagta gtctcgatat cgccgagggc
    10021 attcggaagc acagatgggt gttgacggcc gacgcacgcg tcgtccggat cctggcgatg
    10081 aagtcgcgag agaacacaac cctgtcgagg gcgtgtggcc gagggaggag accgatcatg
    10141 aatggcactt cggatttcgg cacgttcggc cggttcgtcg agacgccggt cgacgagatg
    10201 tcgcccgaga tgaaggaggc ctacgagttc acccggcaac ttcgcggact ggtacccggc
    10261 ccgcacaaga tatggcttgc gaatcccacg ctgtcgaaga cggtcgtgcc gaccggcgcc
    10321 tactttcagc gccagtccac gctgacgaag gccgagatcg agatcgcgac caacatcgtc
    10381 aacggccggt ggcgcagcgc ctacgccaat tacgagcacg agatcgtcgg cgagcgtgac
    10441 ggtcacctcg acccgcgacg tgtcgaagcg ctgatcgccg gtctgccaac atcattcgac
    10501 gatccacggc agcaggtggt ctacgagctt gcaacgacat tggtcgcacc ccgggtagtg
    10561 ccgctcggcc tctaccgccg cgcgaaggaa ctcctcggtg acgcgggcat catcgacatc
    10621 gcggtgctgc tgggctggtt caccatggtg tgtatgacgc tgggcgcctt cgatgtgccc
    10681 gccaacgccg aaggattgga ccagtaacgc cacacagttt cgatcatctc ccaacttgac
    10741 ccgaggatca ccatggccct acccccgctg ccgatcgtca ggttgacgcc agtcgcgttc
    10801 ttcccgcaga agtatttcct ggaaaacctt gccgtgcgtg ccgacgagtc cgtgctcatc
    10861 accgctgtcc tgcagaacga actttggtgg gttccacctg cgaaatccga tgcggtggtt
    10921 cagccggtct tggtgcacgc cttcgaccac ccggtcaccg gtatcgccga ggtcgcgcct
    10981 gatgtgttcg tcgtctgcct caccgaggcc tacaccaccc acgaatcaca tttggtccga
    11041 atcgacttca acggctggac gccgggcacg ccggtatggc cgctggtcat cttcaccttc
    11101 gacgaccgtg tccgcggcct caacggcagc tgccggctcg gacccaacgt gttggcgatc
    11161 gccgactgtt ttgccgggct gatctggcgc gtaaacctag ccgacgacgc gcgcagcgcc
    11221 acggcgcgcg tgtggctttc ggacgacacc atggcgttcg atcccgacag cggcgtgccg
    11281 cccccgccgc aaccgggagt caacggagtc cgctacggcg ttgagaccgg ctacctgtac
    11341 tacacctcaa ccgcgcagaa agtattcatg cgcgttgccg tcgatgcgtc cacccttgat
    11401 ccgatcgcgc agccgcagtt cgtcgccgcc atcgacaacg ccgacgactt ctgcctcgac
    11461 gagaaagccg gattcgctta cgtcacaagg catcgggcaa acaccatcga tcgggtgccg
    11521 ttggcgtccg ggcacggtgg tgaggtccgt cacatcgcgg gggacccgtt cgatgcaacc
    11581 ctcatcgggc cgtccagtgt cgcgtggggc cgcggccgca gcgaccacgg ccgcatcgca
    11641 tatgtcacga ccgacggcgg caccaccgct cccccgcacg gcatcgttcg ccaagccgca
    11701 ctgctgcggg ccgagttcga ctaccccggc cagccccgac ctgccgcacg cgagttatga
    11761 accggcacag caagatcggc acgcgcgtgc cggtcggcga cgcctgccgg cgatgacatc
    11821 accgctgaca ccgggcgggc cctggcgctg ctggatcgag cgggaacgat ccgggaatga
    11881 tgttgtgggg caacaccaat cggttccgca ggaatccgct cgcgcggatg tgccgcgatg
    11941 aacggtgacg ttcaccggca tttggctcgc cgcagtcgat agacgctcga atgtaaaaac
    12001 gctgtcaatc tcccgtaaaa acgctccgag gctatcgcca acctcgggcc ggactgggaa
    12061 gatcttccaa tggccaagca tattggccag tccgttggtg acaggaatga agtcatgccg
    12121 acactagaga cacgcctccg ccaagagttg cgcgattgtg ccgtcgagtt gcggcagttg
    12181 gcgtacacgc tgccgctcgg gataggtgag catgatctgt tgctgttgtc ggatcggatg
    12241 cgcaccgctg ccgatcaagc ggttcgcaaa ggggcctaac cgcccgcctt cgcgcctcgc
    12301 ccatggcagc gcggtgatca gccgcccttg agccggatct tccatcggac gaacccgagg
    12361 tagaccatgc tgatgacgcc cagcatgccc atgtcgaacc accacgcgcc ggcatcgtga
    12421 cgccagtgtg aatccttcgg cgttagtggg ccgggcacca atttggtcag gtcgacggtg
    12481 gaggctgagg tagcgaagcc ccatctcgcc ggtgtggccc acgacatctg gtcgagcgga
    12541 atgcgcccgg tgacgggaat catgccgccg gagaacacca gctgcgacat caccgccacg
    12601 acgagcaagg gcatgatctg ttcgttcgac ttggcgaccg ccgacaacgc cagtccgagc
    12661 atcgccgagg cgacgcaggt cgcggcgacg tcgacgaaca actccaggcc gggcttgccc
    12721 agcgccacgg ccccctgggt ggggccgccc ttgccaatta tcgtgatcgc cgacacgatc
    12781 gccgactgaa cgaccgcgaa cacggtgtag acgcagacct tcgccaacag gtacgcgctg
    12841 gtggagaggc cgaccgcctg ctctcgcaga aatatggcgc gctcaccgat gagatcgcgg
    12901 atcgtcagcg cggtccccat gaagaccgca ccgacattga gcagcaccag gatctgcccg
    12961 ggctcgttgg gcgcgtcgcc cagcgggttc ggtatcccga agccgacgtc gccgggcacc
    13021 gacatcgaca gcgaacccat gatgaagggc agcaccgtga gaaagatgaa gtagccccgg
    13081 tcggaaacga tcaaccggat ctgccgcctc gcgatcgtcg agaactggcg aaacaggctc
    13141 gtgtgcgacg ggtcgcccag ctcggcgggt ttctcagccg gcggcggtgg cgggggtggg
    13201 cccgtccgcg ccaggtaccg tgccttggcc ccatcggggt cgttggcgac agtgctgaag
    13261 atgtcggccc agtttgtcgt gcccatcgcc ggaccgatct ggctgggcgg gccgcagaac
    13321 gccgtcttgc cgccgggggc gagcagcagg acctggtcgc agatgtccag gtaggtcaga
    13381 gagtgggtga cgacaagcac gacacgcccg gcgtcggcca actgccgcag catggtcatg
    13441 acctgccggt ccagcgccgg gtccaaaccg gaggtcggct cgtcgaggat cagcagcgac
    13501 gggccggtga gcagctcgag cgcgaccgag gcgcgtttgc gctgaccgcc ggacaacttg
    13561 tcgacccgag tgtgcagatg ctgactcatc tcgagttcct cgagcacccg ggcgaccacc
    13621 tgctctcggt ccgccttcgt ggtgtccggc ggtagccgaa gttcggcggc gtacatcaat
    13681 gcctgttgca cggtcagctg tccgtgcacc acgtcgtctt ggggcaccat cccgatcctg
    13741 ctgcgcagcg acgcatactc cccgtgcacg ttgtggccct cgaacgcaac ggtccccgtg
    13801 gtcgggtgcg tatagccggc taccaggcgg gcgaaggtgg acttgcctgc accggacgga
    13861 ccgatgaccg cggtgagcgt cccgggctgt gcccccagcg agatgtcttc gagcagtgtc
    13921 ttgttgttct cgatcgtcca cgtcaccccg cgcacgtcga ggccgccggt gcgcgtcgcc
    13981 gtggcggtct cgtcccggcg agcaagcgtg ccgcccgcga acacgaggtc gatgttgccg
    14041 atcgtgacga cgtcaccgtc gtgcagcagg gccgactcca cccgggcgcc attgacgaag
    14101 gtgccgttga tgctgcggtt gtcatggatc tcggtgccgt acggcgtcgg gatcagggtc
    14161 gcgtggtgcc gtgatgccag cacctccgga atgacgatgt cgttgtcgtt ggcacggccg
    14221 atcttgatcg cacccggcgc cgactccatc gccgttcggc ccggccgcag gatcttcatc
    14281 atcgatgtcg cgatgttggt ggagtcgccc acgcgaccgg tctcggcctc ccccgcggga
    14341 gccgggggca ccttcatggg ctgtgcccga tagatcttcg gtgcctgctg cggtccgctg
    14401 ggcatgcccg ctggatgccc agccgtcggg tattgcgccg gtggccccgg agggtagatc
    14461 ggttgcggtc cgctgggcgg atggcttacc tgcggcggat ggcttacctg cggcgcgctg
    14521 ggcggaggcg ggtgctgtgg tccgctgagc gggggcgggg gctgccaggg cgggaccgga
    14581 ggcgggtgtt gaggctggcc aggccagggg gcggcgacct gaggctggct ttgggatacc
    14641 cggtcgcctg gtccgccggc ggggttgagg atcggtatcg acgtggtcag cggcggtcgc
    14701 ccggcggaac cctggtgacg gccgacctca aaggtcagcg ccggaccgtc ggggttgccg
    14761 atattcactc gcaggccgtc ctggatgtcg acgaccggca cgcggcggtt gttgacatac
    14821 agcccgttga ggctgccgtt gtcgatagcc atccatcggc cctggtcgaa ccgcacgatg
    14881 aggtgcgtcc gagagatcag ggggtgtgcc acgcgaacat ccgcgcgaag gtcacgccca
    14941 atgacgacat cggtgccagc tgcgaacgtg cgctcggatc cctcgtaccg cacagtcagc
    15001 acaggcgggg ctggtgggct cacgaggacc aactgtatcg gcagcggcgc cgattacagg
    15061 ggaggtaggg gatgcggcac accgcccggg acacccgacg gagaatttcc gacgatgtgc
    15121 ccgtcgccgg cgaagacgga cgacggcggg tgaatccctg gccgatttcg gcaacgtgac
    15181 cggttgtcag cagaggagat tcggtgacca gcccgctgtt gtcgatgcca cgggtggctc
    15241 aggatatggg catggatgag gcaatgtgcg ctgctccgct gctggcagac cgggtcgcgg
    15301 tggtgacggg cggcggaggc ggaatcggcg ctgcaaccgc gcggttgttc gcccggcacg
    15361 gcgcccgggt ggtgatcgcg gacatcgata cggacctcgc gaaacggacg accggcgaga
    15421 tcacggcgtc gggcggctca gccctggcga ttgtcaccga tgttcgcgac gcagaccagg
    15481 tcgccggctt ggtacgggtg gtgttggatc gctacgggcg ggcagacgtg ctggtgaaca
    15541 acgtgggcca ctggctgcgc cacccgggaa acttcgtcga caccgacccc cagctgtggg
    15601 acgacctcta tcgcatcaat ctgcaccatg tcttctcagt gacccatgcc tttctgccga
    15661 cgatgatcaa tcagcactgt ggcgcgatca tcaatgtgtc gtcagtcgag gggctgcgcg
    15721 gctacccgga ggatcccgtc tacgccgcct tcaaagccgg tgtcatccac ttcacccgca
    15781 gcctcgcggt gcaggtgggc cgcgacggag tgcgggtcaa cgcgatcggc cccgacgtca
    15841 ccgaatctct acaggttccc tactcgcggt ggctgtccgc ccaggagcag acgctgtggc
    15901 cgcaatgggt tccagtcggg cggatggggc tgcccgagga ccaggcccgc gtgatcctgt
    15961 tcctggcctc ggacctgtcg gcgttcgtca ccgggcacac catccccacc gacggcggta
    16021 ctcgcgcggc gggcggatgg ttccgctcgt cgcgccggcc cgaccgggaa tggacaaatc
    16081 gccccgtcgc cccctgaccc cgcccatgcg ttcgctgcta ccgccgggca agcggggcga
    16141 tatgcgctcg aacagcgcgg tggtcggtac ggtctgcaga cgatgtgcga gggcggcggc
    16201 atggccaaca ccccttcatc gagcggctgg gctaggcggc ttcgccgggt agccccttca
    16261 ccgagtggtg cgtcagattc tgagcgacga actccctagt gcgacggtgc cgccgtgcgc
    16321 gaaggcagcc cagtaacccc gcgccttgcg cgccaactca gtgtcaatgg ggttaggttc
    16381 ggggccgagc attggcgcgt cggcccagca ctctggcgag ccgaacagga gcggtagatc
    16441 gatgcagtgg catgcgcgaa gcggtgcccc cggcggtgac cattccacgc gaaatgtcgc
    16501 ggcggtaccg ccatttgatg tccaagactt ggcgagtttg agggctggct ggccaaatat
    16561 ccgcctggtt atcacgtcgc cgagcagtcg ggtgatggcc cgacccggtg ggccaagacg
    16621 ttggagtcgt tgtcctcgcg agtccatggc gacgaacgcg tcagcatcat tgcgggtgta
    16681 gccaacgagt atctcaactc gggatgctgc gtcggcgagt ttgcggcttt cgtcgcgggc
    16741 gggaggtagc gggtccatgc ctgcgatggg cgcgaatggg aacccgccga gcaggccgaa
    16801 tcgctgtgcc gttgtcacag cggcggtctg agcgttgagc agttgctcca cgc
//
LOCUS       DASSGQ010000116        11188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_251397,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000116 DASSGQ010000000
VERSION     DASSGQ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11188
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251397"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VFI55_05505"
     CDS             complement(<1..683)
                     /locus_tag="VFI55_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HET7073522.1"
                     /translation="MANRRLTAAQARRIAVAAQGFSEPRPSGKVTRAHLRQLISRIQV
                     LQLDSVSVAVRAHYAPVFSRLGPYDRDVLDSAAWGPDSTGRSPAPTRLLAEYWAHEAA
                     LMAVDDWPLLRWRMREYRHGRWGTHIVKANPQLAEDIVAAVAELGPSTAGQIEAHLAT
                     EPRGPRGSWWGSRTDTKWVAEALFASGVLTTATRVGFARHYDLVERVLPPSVLAREVD
                     DDEAIRELTL"
     gene            complement(739..1440)
                     /locus_tag="VFI55_05510"
     CDS             complement(739..1440)
                     /locus_tag="VFI55_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HET7073523.1"
                     /translation="MITEPAKTFSRRFRGYDPAAVDAHIEMLTAKQQLLLDDVGSLRA
                     RLKESGSETAALRQEVAVLTDTSPSPHAMQQRMAMMLRRAVDEVAEMQAEARAEADAL
                     IAAAQAEAEAAQREHEELLEDMAARLQAQEAEYEETKKKLEAELARMRAETQSTIDEA
                     RRDARQEADQQREQARRAVDEAGQRRIKILEQLMDVYRDLETVPATLESAYRDQSNPP
                     EANAATRLDRKVSTG"
     gene            complement(1519..2031)
                     /locus_tag="VFI55_05515"
     CDS             complement(1519..2031)
                     /locus_tag="VFI55_05515"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET7073524.1"
                     /translation="MTEVGLVWAQSTSGVIGRGGDIPWRVPEDLARFKQVTMGHTVVM
                     GRRTWDSLPAAVRPLPGRRNVVLSRQTGFMAHGAEVLGSLEQALIEPETWVIGGEQIY
                     RLALPLATRCEVTEVDVDLPRDDDDALAPLLDEEWLGETGEWQLSRTGMRYRFHSYCR
                     PSALVSAPTA"
     gene            complement(2108..2908)
                     /locus_tag="VFI55_05520"
     CDS             complement(2108..2908)
                     /locus_tag="VFI55_05520"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET7073525.1"
                     /translation="MPIVTPYEDLLRLVLETGAPKADRTGTGTRSLFGQQLRYDLAAG
                     FPLLTTKKVHFKSVVYELLWFLRGDSNVAWLHEHGVTIWDEWASSTGDLGPIYGVQWR
                     SWPTPSGEHIDQISAALDLLRTDPNSRRIIVSAWNVGDIPQMALPPCHAFFQFYVADR
                     RLSCQLYQRSADLFLGVPFNIASYALLTHMMAAQAGLGVGEFVWTGGDCHIYDNHVEQ
                     VRTQLGRDPRPYPELVLADRDSIFDYTYDDVVVKNYDPHPAIKAPVAV"
     gene            3007..3747
                     /locus_tag="VFI55_05525"
     CDS             3007..3747
                     /locus_tag="VFI55_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7073526.1"
                     /translation="MPSITDTVSTPDGTCPVRLFTPDGPGPWPGVVMFPDAGGVRETF
                     YEMAAKLAGFGYAVLLPDVYYRNGDWAPFEMASVFGDEKERNRLMSMIGSLTPDKVTS
                     DTAAFFDYLAARPEVSGDRFGVCGYCMGGRISVMLAGRLPDRVAAAASFHGGGLVTDS
                     PDSPHLLADAMSATVYVGGAENDPSFTADHAEQLDKALTAAGVQHTIEWYQAAHGFAV
                     PDNAPYDAAADERHWTAMTETFGAALPR"
     gene            complement(3736..4974)
                     /locus_tag="VFI55_05530"
     CDS             complement(3736..4974)
                     /locus_tag="VFI55_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014985301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7073527.1"
                     /translation="MRVLLSTYDSRGGVEPLVALAVQLQALGAQVSVCAPPDEEFAER
                     LAGFGVPLVPFGDSVRAMLTRATPPSEAELRQHLDALIAAQFDIVAAAAEGGDAVVAT
                     GPPWTAAGARSVAEKLGIRYVYASYHPNHLPSPHHPPAEHADLRYPLSQADNRMLWDQ
                     NARNTNAAVGKTLNGHRASIGLPPVDNVRDYAFTDRPWLAADPFLGPWQQPAGIDVVQ
                     TGAWLLRDERPLPAELVAFLDAGTAPVYVGFGSMRAPKDTARAAIEAIRAQGHRALLA
                     RGWADLAPIDDCDDCFAVGEVNQQALFTRVAAVVHHGGAGTTTTAARAGAPQVVVPQG
                     KDQLYWAGRVAELGIGTAHVGLPLSRQSLAAAFATILTPETRAHASGVAGNVRTDGAA
                     VAARRLVEAVSQSGAAISTR"
     gene            5675..6088
                     /locus_tag="VFI55_05535"
     CDS             5675..6088
                     /locus_tag="VFI55_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7073528.1"
                     /translation="MEALHEMPALVILERLPAPALAVDCNGNILFANVAFCDMLGYAS
                     DALLSMTFEEVFHSLPADDRWVALIGTEAPRLVELQHASGHAVWASMSKSAMRRRDDT
                     VALVTFHDRTEQLWDERPAPDQDGGKFTKRDKRAR"
     gene            complement(6043..7149)
                     /locus_tag="VFI55_05540"
     CDS             complement(6043..7149)
                     /locus_tag="VFI55_05540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7073529.1"
                     /translation="MTIGDLLYSDTEEALRDSVRHLFAERCPPESVLRAYDPVPPDFS
                     GVWRTLAAELGVAGLLVPESLGGAGAGAREAAVVMEEVGRAVAPVPFLSSAVLATVAL
                     LAAGDTETVPALAQGELTAALVVPLSTAPGDPVAGVSAAGGGLSGEVSSVAGAGEADV
                     LVVPVAGPDGAELHTVSRTAAGVDVSPVLALDMTRPLANVRFSGAASTRLGPAHNAVA
                     EALQSGAALLASEQLGVAQWCFDTTLAYVKQRKQFGRAIGSYQSIKHRLADLWFEVGA
                     ATAVARHAADACARSDATPKDAATATAVAQAYCSDIAVHAAEECVQMHGGIGMTWEYP
                     AHLYLKRAKSDQLAFGTAYRQRARLSRLVNLPPS"
     gene            complement(7146..8393)
                     /locus_tag="VFI55_05545"
     CDS             complement(7146..8393)
                     /locus_tag="VFI55_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7073530.1"
                     /translation="MSSADVAADLRARVRALLDEHDPTTTDPREFLGARYDAGLAWVY
                     LARGFGGLDLPRKAQELVDAELAAAGAPAGGTVKNFIGMGMAAPTIAAFGTDEQKRKF
                     LRPLFTGEHVYCQLFSEPGAGSDLAGVATRAVRDGDEWIVNGQKVWTSMAQHAQMAIL
                     VARTDPTVPKHAGLTYFLCDMTQPGVDVRPLRQITGEAEFNEVFLTDVRVPDANRLGP
                     EGGGWRVATTTLNNERVAIGSRPGAPREGGMIGKVTKAWRDEPGLRDPAMHDDLMRLW
                     VDAEVLRLAGERLRQQARAGQPGPEGAGMKVAFAKLAQAISSFDLELHAESGLHYDDW
                     TMRRTEVVDLIGREPGYRYLRARGNSIEGGTSEILRNTISERILGLPGEHRVDKDRPW
                     SDLNKSVLNKSDLNKSDLNKGGR"
     gene            complement(8393..9172)
                     /locus_tag="VFI55_05550"
     CDS             complement(8393..9172)
                     /locus_tag="VFI55_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7073531.1"
                     /translation="MTVLDLTGRTAIITGASRGIGLAIAEQLAVAGANVVLTARTQEA
                     AEAAAARVGERALGVGAHAVDEDAARRCVDLTLERFGSVDILVNNAGTNPAYGPLIDQ
                     DHARFSKIFDVNLWAPLLWTSLVVKSWMGEHGGAIVNTASIGGLHQSPTMGLYNATKA
                     ALIHVTKQLALELSPRVRVNAIAPGVVRTRLAEALWKDHEDPLASSIALGRIGEPADV
                     ASAVTFLVSDAASWITGETMVIDGGLLLGPAQGFQVRPEAT"
     gene            9296..9625
                     /locus_tag="VFI55_05555"
     CDS             9296..9625
                     /locus_tag="VFI55_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PGRS family protein"
                     /protein_id="HET7073532.1"
                     /translation="MKPLVVAGAVAAAIAAAPTAAADVFAIGPAMTNPAQTHVVIKDD
                     PGGAGCDANGNCGSGGQNGGPGGGPGGQGCLPGVGCGSGGQFAGPGGVPGGTGCLPGV
                     GCGSGHG"
     gene            complement(9640..>11188)
                     /locus_tag="VFI55_05560"
     CDS             complement(9640..>11188)
                     /locus_tag="VFI55_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET7073533.1"
                     /translation="ATDGESRSSAVDLDRPRADLDEVRERHLLTLDAGRQAAVAKRHK
                     QGRRTARENIDDLVDAGSFVEYGALAIAAQRSRRSEEDLIANTPADGLIAGQATIGAD
                     RFGRAAAEAVVVSYDYTVLAGTQGMRNHAKTDRVFDLATRKDLPVVLFAEGGGGRPGD
                     TDVGGAAGLDVPTFRMLAGLSGRVPLVSIVSGRCFAGNAALAGVCDVIIATPDANIGM
                     GGPAMIEGGGLGVYPPEAIGPIDVQRRNGVVGLVARDEAHAVSLAKRYLSYFQGNLQN
                     WSAPDPRLARHVVPENRLRAYHVHRAIESIVDVGSVLELRPEYGVGIVTALVRVEGVA
                     YGLIANSTHHLGGAIDAEAADKAGDFLALCESFRLPVVSLCDTPGFMIGPDAEKEAAV
                     RRFGRMFVLGARLSVPLGMIVLRKGYGLGAMAMAGGSFRAPQFTVAWPTGEIGGMGLE
                     GAVRLGFSKELAAEPDPAERQNLFDRLVAAAYQHGKALRAATTFELDDVIDPSASRAW
                     ITRLSGD"
BASE COUNT         1766 a         3939 c         3665 g         1818 t
ORIGIN      
        1 agcgtgagct cgcggatggc ctcgtcgtcg tcgacctcgc gagccagcac gctcggcggc
       61 agcacccgct ccaccaggtc gtagtggcga gcgaaaccga cccgggtggc ggtcgtgagc
      121 acgccggaag cgaacagcgc ctcggcgacc cacttggtgt cggtgcgact gccccaccag
      181 cttccccttg gcccgcgcgg ctcggtggcc agatgcgctt cgatctgccc ggccgtgctg
      241 ggcccaagtt cggcgacggc ggcgacgatg tcctcggcga gctgcggatt ggccttgacg
      301 atgtgggtgc cccagcggcc atgccggtac tcgcgcatcc gccaccgcag cagcggccag
      361 tcatcgacgg ccatcagcgc ggcctcgtgc gcccagtatt cggccagcag ccgcgtcggc
      421 gccggacttc ggccagtcga gtccggaccc caagccgcgc tgtccagcac gtcgcggtca
      481 taggggccga gccgactgaa caccggcgcg tagtgggcgc gcaccgccac cgacaccgaa
      541 tccagctgca gcacttggat gcgggatatc agctggcgca ggtgcgcacg ggtaaccttg
      601 ccgctggggc gtggctcgct aaatccttgg gccgcaacgg ctatccgtcg ggcctgcgcg
      661 gccgtcagcc tacggttcgc gatcgacacg agaataccgg tcctttcggc tgccctcagc
      721 gacgaagcca gcgcggactc accccgtgct gactttccgg tccaaccgcg tcgcggcatt
      781 cgcctccggc ggattacttt ggtctcggta tgccgactca agagtggccg gaaccgtttc
      841 caggtcgcgg tacacgtcca tcagttgttc caagatcttg attcgccgct gccccgcctc
      901 gtccaccgcc cgccgggcct gctcgcgctg ctgatcagct tcctgccggg cgtcccgccg
      961 tgcctcgtcg atggtcgatt gggtttcagc gcgcattctg gccaactcgg cctcgagctt
     1021 tttcttggtt tcctcgtact cggcttcttg ggcttgtagc cgcgccgcca tgtcctcgag
     1081 cagctcctcg tgctctcgtt gcgcggcctc ggcctcggcc tgggccgccg cgatcagcgc
     1141 gtctgcctcg gcccgcgcct cggcctgcat ctcggcaacc tcgtcgacgg cacgccgcag
     1201 catcatcgcc atccgctgtt gcatcgcgtg gggcgagggt gaagtgtccg tgaggaccgc
     1261 gacctcctgg cgcagcgcgg ccgtttcact cccggattcc ttcaaccgtg ccctcaggct
     1321 cccgacgtcg tcgagcagca actgttgttt ggccgtcagc atctcgatgt gggcatcgac
     1381 cgcagccgga tcgtagcccc tgaacctgcg cgagaacgtc tttgcgggtt cggttatcac
     1441 tgccaattcc cccttctgac ttctgaaaac gccttcaaga cccgtcgaga ccgaccccaa
     1501 gtaaccgagt atctcaagtc acgccgttgg cgcggacacc agcgctgacg gccgacagta
     1561 actgtgaaac cggtagcgca tccccgtgcg gctgagttgc cactcccccg tctcgcccaa
     1621 ccactcctcg tcgagcaagg gcgccagcgc gtcgtcgtca tcgcgcggca ggtcgacgtc
     1681 gacctcggtg acctcgcacc gggtggccag cggcagcgcg agccgataga tctgctcgcc
     1741 gccgatcacc cacgtctcgg gctctatcag tgcctgctcg agtgaaccga gcacctccgc
     1801 tccgtgcgcc atgaagccag tctgacggga cagtacgaca tttctgcgcc ccggcagcgg
     1861 ccgcacggca gccggcagcg aatcccacgt ccgccggccc atgaccaccg tgtgtcccat
     1921 ggtcacctgc ttgaagcggg ccaggtcttc gggtacccgc cacgggatgt cgccgccgcg
     1981 gccgatgaca ccagacgtcg actgagccca gacgaggccc acctcggtca tccgcgccac
     2041 tctcccccgc aagcgggcgg tacccccatg ctcccggcta caccgggacc atcgtcatcg
     2101 gcgcgcgtca tactgcgact ggagctttga tcgccgggtg cggatcgtag ttcttcacca
     2161 cgacgtcgtc gtaggtgtag tcgaagatcg aatccctatc cgccagaacg agttccgggt
     2221 aaggacgcgg gtcgcggccg agttgggtcc gcacctgttc gacatggttg tcatagatgt
     2281 ggcagtcacc gccggtccag acgaactcgc cgacgcccaa cccggcctgg gcggccatca
     2341 tgtgggtgag cagcgcgtag ctggcgatgt tgaacggcac gccgagaaac aggtccgcgc
     2401 tgcgctggta gagctgacag ctcagccgcc ggtcggcgac gtagaactgg aagaaggcgt
     2461 gacacggcgg cagcgccatc tgcgggatgt cgccgacgtt ccatgccgac acgatgatcc
     2521 gccgcgagtt cgggtcggtg cggagtaaat ccagcgcggc gcttatctgg tcgatgtgct
     2581 cgcccgacgg tgtcggccac gaccgccact gcactccgta gattggcccg agatcgcctg
     2641 tgctgcttgc ccattcgtcc cagatggtga cgccgtgctc atgcagccat gcgacgttgg
     2701 agtcgccgcg cagaaaccac agcaactcgt agaccacgga tttgaaatgc accttcttgg
     2761 tggtgagcaa cgggaagccg gcggccaggt cgtagcgcaa ctgctgtccg aagaggctgc
     2821 gggtaccggt gccggtgcga tcggctttgg gggcgcccgt ttcgagcacc aggcgcagca
     2881 gatcctcgta gggagtgacg atcggcacgc ggccagctta gcggcgatcg caagcacggc
     2941 gaagccgggc gcggcgggtc gacgccgatg aatttgcctt caattccggg gagtacaacg
     3001 gatggcatgc ccagcatcac cgacaccgtc agcactcccg acgggacctg ccccgtccgc
     3061 ctgttcaccc ccgacgggcc cggaccctgg ccgggtgtgg tcatgttccc cgacgccggc
     3121 ggtgtgcgtg agaccttcta cgagatggcg gccaagctcg ccggattcgg ctacgcggtg
     3181 ctgcttcccg acgtgtacta ccgcaacggc gactgggccc cgttcgagat ggccagcgtg
     3241 ttcggggacg aaaaagagcg caaccggctg atgtccatga tcggcagcct gacgccggac
     3301 aaagtgacca gcgacaccgc cgccttcttc gattacctgg ccgctcggcc cgaggtatcc
     3361 ggtgaccgat tcggcgtttg cggctactgc atgggtgggc ggatctcggt gatgctggcc
     3421 ggccgccttc ccgaccgcgt cgccgcggcg gcgtccttcc acggcggcgg cctggtgacc
     3481 gacagtccgg acagcccgca cctgctggcc gacgcgatgt ccgccaccgt gtacgtcggg
     3541 ggcgccgaaa acgacccgtc gttcacggcc gaccacgccg agcagctgga caaagcgctc
     3601 accgcggccg gcgtgcagca caccatcgag tggtaccaag ccgcccacgg cttcgcagtc
     3661 ccggacaatg ccccctacga cgccgccgct gacgaacggc attggacggc aatgacggaa
     3721 accttcggag cggcgttacc gcgttgagat ggcggcgccc gactgactga ctgcctccac
     3781 cagccgcctc gcggccacgg ccgccccgtc ggtgcggaca ttgccggcga cgccactggc
     3841 gtgtgcgcgg gtctcggggg tcaagatggt cgcgaacgcg gcggcgagcg actgcctgct
     3901 caatggaaga ccgacgtgcg cggttccgat gcccagctcc gccacccgac cggcccagta
     3961 caactggtcc ttgccctggg gcacgaccac ctggggtgcc ccggcccgcg cggccgtcgt
     4021 ggtggtgccg gcgccgccgt ggtgcacgac ggcggccacc cgggtgaaca atgcctgctg
     4081 gttgacctcg ccgacggcaa agcagtcgtc gcagtcgtcg atcggcgcca agtcggccca
     4141 gccgcgcgcc aggagtgcgc ggtggccctg cgcacggatc gcctcgatgg ccgcccgggc
     4201 ggtgtccttc ggggcgcgca tgctgccgaa gcccacgtac acgggcgccg ttccggcgtc
     4261 caagaacgcc accagctcgg caggcagtgg acgttcgtcg cgcagcagcc acgcaccggt
     4321 ctggacgaca tcgatcccgg ccggctgctg ccacgggccc aggaaggggt ccgccgccag
     4381 ccacgggcgg tccgtgaagg cgtagtcacg gacattgtcc accggtggca gaccgatcga
     4441 ggcgcgatgg ccgttgaggg tcttaccgac ggccgcgttc gtgttgcggg cgttctggtc
     4501 ccacagcatc cggttgtcgg cctgactcag cgggtatcgc aagtctgcgt gctcggccgg
     4561 cgggtggtgc ggcgacggca gatggttcgg gtgatagctc gcgtacacgt aacggatgcc
     4621 cagcttttcg gccaccgatc gcgcgcccgc cgcggtccat ggcgggccgg tcgccaccac
     4681 cgcgtcacct ccctcggccg cggcggcgac gatgtcgaac tgcgcggcga tcaacgcgtc
     4741 caggtgttgg cgcaactcag cctcagacgg cggcgtcgcc cgggtgagca tcgcgcgcac
     4801 ggagtcgccg aagggcacca gcggcacgcc gaaaccggcc agccgctcgg cgaactcctc
     4861 gtcgggcggc gcgcacaccg acacttgcgc gccgagtgcc tgcaactgca ccgccaatgc
     4921 gaccagcggt tctacgcccc cgcgcgaatc gtatgtcgat aacaacaccc gcatttgtgt
     4981 tcgcgttact cccgtctcca caggctgtgg tgtcggcaac cgattgtgca gcaccaccct
     5041 gggtcgcgcg aggtaagcac gtcgcaagaa gtgcctcccc gtggccctac gacgactgct
     5101 cgggcaaaac ttccgcctgc ggacaagcca ccgctttcgt ccacttgacg gctagaacta
     5161 tcgtagatct gtaaaagtgt ttcctaggtt ggctggtgcc cgagattggc ctgggcagca
     5221 gcgctgtcag ctcagcgcgg caacggatcc gagttgggcg cgatcccggg ctatccgtgg
     5281 gttggtggac acctttgggc gcagccttcg cgtgacgacc aacacggagg aagcgttggc
     5341 cgaactttgc tatggtgcga tcatccatca tgtcgggtcg acgaaatcaa ttcttcagct
     5401 aataaattat gctaagcgat tccagcactg accccatcac aaccccgtga aataattgtg
     5461 catgtggcaa atagcaaatt tgatcaactc agtatagact tcccgatgga cgtaccataa
     5521 aatttgctgt cagcgacatg gggtgggtgg gcaccgccat atcgaggata gaacgagcgc
     5581 aatgacgggg tgatccgtgg ttacgaacgg tcccacagcc gctggaaatc agcgtgtcga
     5641 gcgccgacgt ccgagccctg ccgaccagtt gccgatagaa gcactgcatg agatgcccgc
     5701 gctggtcatc ctggagcggc tgccggcgcc ggccctggcg gttgactgca acgggaacat
     5761 cctgttcgcc aacgtcgcgt tctgcgacat gctgggctac gcgtccgatg cgttgctatc
     5821 gatgacgttc gaagaggtct ttcacagcct gcctgccgac gaccgctggg tcgcgctgat
     5881 cggaacggaa gcgccgcggc tcgttgagtt gcaacacgcc agcgggcacg cggtgtgggc
     5941 cagcatgagc aagtcggcca tgcggcgccg tgatgacacc gtcgcactgg tcaccttcca
     6001 tgaccgcacc gaacagctgt gggacgagcg acccgcgccc gatcaggacg gcggcaagtt
     6061 caccaaacgg gacaaacggg cgcgctgacg gtaggcggtg ccgaaggcca actggtcgct
     6121 cttggcgcgc ttgaggtaca ggtgcgccgg atattcccag gtcattccga taccgccatg
     6181 catttgcacg cactcctctg ccgcgtgcac ggcgatgtcg ctgcagtagg cctgcgcgac
     6241 ggcggtcgcg gtggccgcgt ctttcggggt cgcgtcgctg cgcgcgcacg cgtcggcggc
     6301 gtgccgggca accgccgtcg ccgcgccgac ctcgaaccac aggtccgcca gccggtgctt
     6361 gatcgactgg taggagccga tcgcacgccc gaactgcttg cgctgcttga cataagccag
     6421 cgtggtatca aaacaccact gggccacacc gagttgctcg gaggcgagca gggccgcccc
     6481 gctctgcagc gcttcggcca cggcgttgtg cgccgggccc agccgggtcg acgccgcgcc
     6541 cgagaagcgc acgttcgcaa ggggtctcgt catatccaac gccagcacgg gcgacacgtc
     6601 gacaccggcc gcggtccggg agacggtatg cagctcggct ccgtccgggc cggcgaccgg
     6661 caccaccagc acgtcggctt ccccggcacc cgcgacgctg cttacctcgc cgctcagtcc
     6721 gccaccggcc gcactcaccc ccgcaaccgg gtcgcccggc gcggtggaca gcggcaccac
     6781 cagcgcagcg gtgagctcac cctgggccag cgccggcacg gtctcggtgt caccggcagc
     6841 cagcagcgcg accgtggcca gcaccgcgct ggacaagaag ggcacgggcg ctacggcccg
     6901 gccgacctcc tccatcacga ccgcggcctc gcgggcgccc gcgccggcgc cgccgagcga
     6961 ctcgggcacc agcagtccgg ccacccccag ctcggcggcc agcgtccgcc agacacccga
     7021 aaagtctggt ggcacagggt cgtaggcccg gagaaccgat tcgggtgggc accgctcggc
     7081 gaacaggtgg cggacgctgt cgcgcagcgc ctcctcggtg tcggagtaca gcaagtcgcc
     7141 gatggtcatc ggcccccctt gttcaggtcc gacttgttca agtccgactt gttcaagacc
     7201 gacttgttca agtccgacca aggtctgtct ttgtcgacgc ggtgttcacc cggcaggccg
     7261 aggatccgct ccgagatggt gttgcgcagg atctccgagg tgccgccctc gatcgagttg
     7321 ccgcgcgccc gcaggtaccg gtacccgggc tcacgaccga tcaggtcgac gacttcggtt
     7381 ctgcgcatgg tccagtcgtc gtagtgcagc ccggactcgg catgcagctc gaggtcgaaa
     7441 ctcgaaatgg cctgcgccag tttggcaaag gccaccttca tgccggcgcc ctcgggtccg
     7501 ggctgacccg ctcgcgcttg ttgtcgcagc cgttcaccgg ccagccgcag cacctccgcg
     7561 tcgacccata gccgcatcag gtcgtcgtgc atcgcggggt cacgcaaccc cggctcatcg
     7621 cgccaggctt tggtgacctt gccgatcatc ccgccctcgc gcggcgcgcc gggccgcgaa
     7681 ccgatggcga cccgctcgtt gttgagcgtg gtcgtcgcga cccgccagcc gccgccttcg
     7741 gggcccagcc gattcgcgtc gggcacccgg acgtcggtca ggaacacctc gttgaactcc
     7801 gcctcgccgg tgatctgccg cagcggccgc acgtcgacac ccggttgcgt catgtcgcac
     7861 aaaaagtagg tgagcccagc gtgtttgggc acggtcgggt cggtccgcgc aaccagaatg
     7921 gccatctggg cgtgctgcgc catcgacgtc cacaccttct gcccgttgac aatccactcg
     7981 tccccgtcgc ggaccgcgcg ggtggccacc cctgcgaggt ccgaccccgc gcccggctcg
     8041 ctgaacaact ggcagtagac gtgctcgccg gtgaacaacg ggcgcaggaa cttccgcttc
     8101 tgttcgtcgg tcccgaacgc cgcgatggtc ggcgccgcca tgcccatccc gatgaagttc
     8161 ttgacggtgc cgcccgccgg cgcacccgcg gcggcgagct cggcgtcgac gagttcctga
     8221 gctttccggg gcagatccag cccgccgaaa ccccgcgcca ggtaaaccca ggcaagcccc
     8281 gcgtcgtacc gcgcgccaag gaattcccgc gggtcggtgg tggtcgggtc gtgttcgtcg
     8341 agcagtgcgc gcacccgtgc ccgcaggtcg gccgcgacgt ctgccgaact cattacgtgg
     8401 cctcgggtcg aacctggaat ccctgcgccg ggccgagcaa caggccgccg tcgatgacca
     8461 tcgtctcgcc ggtgatccag ctcgccgcat ccgaaaccaa aaaggtgacc gcacttgcca
     8521 catcggccgg ctcaccgatg cgcccgagcg caatcgacga ggccagcggg tcctcgtggt
     8581 ccttccacag cgcctcggcc agcctggtgc gaaccactcc cggagcgatc gcattgaccc
     8641 ggacacgcgg tgaaagttcc agcgcgagtt gcttggtgac gtggatcagc gccgccttgg
     8701 tggcgttgta cagccccatc gtcggcgatt ggtgcaggcc gccgatcgag gcggtgttga
     8761 cgatggcacc gccatgctcg cccatccacg acttgacgac aagcgaggtc cacagcagcg
     8821 gcgcccacag gttgacgtcg aagatcttgc tgaaacgggc gtggtcctgg tcgatcagcg
     8881 gaccgtacgc ggggttggtt ccggcgttgt tgaccaggat gtcgacgctg ccgaagcgct
     8941 ccagcgtgag atccacgcag cgtcgcgcgg catcctcgtc gacggcgtga gcgccgaccc
     9001 cgagggcccg ctcaccgacc cgcgccgcgg ccgcctccgc cgcctcctgc gtgcgggcgg
     9061 tgagcaccac attggcgccg gcgactgcca gttgttccgc gatcgccagc ccgatcccgc
     9121 gcgaggcgcc ggtgatgatc gcggtacggc cggtcaagtc cagtacggtc attttggtcc
     9181 gccttctgct gcattgaaag ccggactgcg ccagcgagaa ccccgatctt gcagacgatg
     9241 ttttgcacgg gtgtctacgt ggtatgcgtc aggcatgtta gcgaaactaa ccctcatgaa
     9301 accattggtg gtcgccgggg ccgtcgccgc ggcgatcgcc gccgcaccaa cggccgctgc
     9361 cgatgtcttt gctatcggtc cggcgatgac caacccggcg cagacgcacg tcgtcatcaa
     9421 ggacgacccg ggcggcgccg gctgcgacgc caacggcaac tgtggatccg gcggccagaa
     9481 cggcgggccc ggcgggggtc cgggcggcca gggctgcctc ccgggcgtcg gctgcggctc
     9541 cggcggccag ttcgccggtc ccggcggcgt gccgggcggc accggctgtc tgccgggcgt
     9601 cggctgcggt tccggtcacg gataacagcg ggtcgatcct cagtctcccg acaacctggt
     9661 aatccaggcc cgggaggctg acggatcgat gacgtcgtca agttcgaacg tggtggccgc
     9721 ccgcagcgct ttgccgtgct ggtaggcggc cgcgacgagg cggtcgaaca ggttctggcg
     9781 ttcggccgga tcgggttcgg cggcgagttc cttgctgaag ccgagccgca ccgcgccttc
     9841 caggcccatg ccgccgatct ccccggtcgg ccaggccacg gtgaactgcg gcgcgcggaa
     9901 ggagccgccg gccatcgcca tcgcgccgag cccatagccc ttgcgcaaga cgatcattcc
     9961 gagcggcacg ctcagccgcg cccccagcac gaacatccgg ccgaagcggc gcaccgcggc
    10021 ctctttttcc gcgtcgggac cgatcatgaa gccgggggtg tcgcacagcg agaccaccgg
    10081 caaccgaaac gactcgcaca gggcgagaaa gtcgccggcc ttgtcggccg cttcggcgtc
    10141 gatcgcgccg ccgagatggt gggtgctgtt ggcgatcagc ccgtaagcca caccctcgac
    10201 gcggacgagt gcggtgacga ttcccacgcc gtactccggc cgcagctcca gtacagagcc
    10261 gacgtcgacg atcgactcga ttgcccgatg cacgtggtag gcccgcaacc ggttctccgg
    10321 caccacatgc cgcgccagcc gcggatccgg cgcagaccag ttctgcaagt tgccctgaaa
    10381 gtacgacaga taccgcttgg ccagtgacac cgcgtgcgcc tcgtcgcggg cgacgaggcc
    10441 caccacgccg ttgcgtcgct gcacgtcgat cggcccgatc gcctccggcg ggtacacccc
    10501 gagtccgccg ccctcgatca tcgccggccc gcccatcccg atgttggcgt ccggggtcgc
    10561 gatgatcacg tcgcacaccc cggccagcgc tgcgttgccg gcgaagcaac ggccggacac
    10621 gatcgacacc agcggcaccc ggccgctcag cccggccagc atccgaaagg tcggcacgtc
    10681 cagcccggcg gcgccgccga cgtcggtgtc cccgggtcgg ccaccaccgc cctcggcgaa
    10741 cagcaccacc ggcaggtctt tccgtgtggc caggtcgaag acacggtcgg ttttggcatg
    10801 gttgcgcatg ccctgagtcc ccgcgagcac cgtgtagtcg taggacacca cgaccgcctc
    10861 ggccgcggct cgcccgaagc gatccgcgcc gatggtggcc tggccggcaa tcagtccgtc
    10921 ggccggggtg ttggcgatca ggtcctcttc cgagcgccgg ctgcgctgcg cggcgatggc
    10981 cagcgcgccg tactcgacga agctgcccgc atcgacgagg tcgtcgatgt tctcccgggc
    11041 ggtgcggcga ccttgcttgt gccgcttggc gacggcggcc tgccggcccg cgtcgagggt
    11101 gagcaggtgt cgctctcgga cctcgtcgag gtcggcgcgc ggccgatcga gatccactgc
    11161 cgacgaacgt gattcgccat cggtcgcg
//
LOCUS       DASSGQ010000117         4356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_737735,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000117 DASSGQ010000000
VERSION     DASSGQ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737735"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..556
                     /locus_tag="VFI55_05565"
     CDS             5..556
                     /locus_tag="VFI55_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7073534.1"
                     /translation="MASAPVQPGLFPDDSVPDELVGYRGPSACQIAGITYRQLDYWAR
                     TSLVVPSIRSAAGSGSQRLYSFKDILVLKIVKRLLDTGISLHNIRVAVDHLRQRGVQD
                     LANITLFSDGTTVYECTSAEEVVDLLQGGQGVFGIAVSGAMRELTGVIADFHGERADG
                     GESIAAPEDELASRRKHRDRKIG"
     regulatory      635..732
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            906..3743
                     /gene="gcvP"
                     /locus_tag="VFI55_05570"
     CDS             906..3743
                     /gene="gcvP"
                     /locus_tag="VFI55_05570"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HET7073535.1"
                     /translation="MPDQSTFAARHIGPDSQAIAAMLAVIGVDSFEELASKAIPSGIL
                     DKLTDSGAAPGLDTLPPAASEAEALAELRALADSNTVAVSMIGQGYYDTLTPPVLLRN
                     IMENPAWYTAYTPYQPEISQGRLEALLNFQTMVADLTGLEVANASMLDEGTAAAEAMT
                     LMHRAARGTSKRLAVDGDVFAQTAAILATRAKPLGIEIVTADLRDGLPDGEFFGVITQ
                     LPGASGRITDWSALVAQAHDRGALVAIGADLLALTLLTPPGEIAADVAFGTTQRFGVP
                     MGFGGPHAGYLAVHTKHARQLPGRLVGVSLDADGSPAYRLALQTREQHIRRDKATSNI
                     CTAQVLLAVMAAMYASYHGSDGLTEIARRVHSHAATIAAALGDALAHDAFFDTVLARV
                     PGRADEVVAAAKANGINLWRVDADHVSVACDEATTGAHVAAVLDAFGVASTFAAREPA
                     AADIATRTSDFLTHPAFTQYRTETAMMRYLRTLADKDIALDRSMIPLGSCTMKLNAAA
                     EMESVTWPEFARQHPFAPASDIPGLRRLIADLESWLVAITGYDAVSLQPNAGSQGEYA
                     GLLAIHDYHASRGEPHRTICLIPSSAHGTNAASAALAGMRVVVVACHDNGDVDLDDLR
                     AKVGEHTEKLSTLMITYPSTHGVYEHDIADICAAVHDAGGQVYVDGANLNALVGLARP
                     GKFGGDVSHLNLHKTFCIPHGGGGPGVGPVAVRSHLAPFLPGHPYAPELPEGHPVSSA
                     PYGSASILPITWAYIRMMGADGLRAASLTAITSANYIARRLDEYFPVLYAGDNGMVAH
                     ECILDLRGITKSTGVTVDDVAKRLADYGFHAPTMSFPVAGTLMVEPTESETLTEVDAF
                     CEAMIAIRSEIDRVGAGEWPVDDNPLRGAPHTAECLVVSHWDHPYTREEAAYPLGKAF
                     RPKVWPPVRRIDGAYGDRNLVCSCPPVEAFA"
     gene            complement(3760..>4356)
                     /locus_tag="VFI55_05575"
     CDS             complement(3760..>4356)
                     /locus_tag="VFI55_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7073536.1"
                     /translation="KVIGEFVDHLGLDDYLTMGQDWGGPISMAVDVERADRVRGVILG
                     NTWFWRADELTTKIFSLVMSSPPMQWAILQRNYFVEHLIPLGTAHRLSAVVMEHYRAV
                     QPSPSTRKGVAEMPKQILAAHPLLTRLGREVPAKLGAKPALLVWGMKDFAFRPGPTLP
                     RMRAAFPDHVLVELPEAKHFIQEDEPGQIAAAIVERFG"
BASE COUNT          726 a         1542 c         1407 g          681 t
ORIGIN      
        1 caccgtggcc agcgcgcccg tgcaacccgg gctgtttccc gacgactccg tgcccgacga
       61 attggtcggc taccgcgggc ccagcgcctg ccagatcgcc ggaatcacgt atcgccagct
      121 ggactactgg gcccgcacgt cgctggtggt tccatcgatc cgcagcgccg ccggatccgg
      181 cagccaacgg ctctattcgt tcaaggacat cctggttctc aagatcgtca agcggttgct
      241 cgacaccggt atctcgctgc acaacatccg ggtcgccgtc gaccatctcc gccagcgcgg
      301 cgtccaggac ctggccaaca tcaccctgtt ctccgacggc accacggttt acgagtgcac
      361 atcggccgaa gaggtcgtcg acctgctgca gggcggacag ggcgtgttcg gcatcgccgt
      421 gtcgggcgcc atgcgcgagc tgaccggcgt catcgccgac ttccacggtg aacgcgccga
      481 cggcggcgag tcgatcgccg cccctgagga cgagctggcg tcgcggcgca agcaccgcga
      541 ccgcaagatc ggctagcacc gcaccgttgc cgcgtcgggc aaacgcgatc gctcgcggcg
      601 aaatggagac ggccgcgtaa actagtccaa gcgtcgcact tgcgcgggag agctccgtga
      661 cggccagtca cggacgccga aggagcaaca cctctccgtc aacctctcag gcacccggac
      721 cgcgacagtt aacgacgcct ctggaaagcg gtggagaccg gcaacggtcc acgcccgccg
      781 atggggaaag gcgctcaggt agtacctagc gccgaatctc tcaggcgcct ggcgaacagg
      841 taaagacaga gggagagggc cgctagtcct ctgcctttgt catgcctacc tcaggagttt
      901 cgccagtgcc cgaccagtca actttcgcag cccggcacat cggcccggac agccaggcca
      961 tcgcggccat gcttgctgtc atcggtgtcg actcgttcga agagctggca tccaaggcga
     1021 tcccgtcggg cattctcgac aagctcaccg acagcggtgc cgcgcccggt cttgatacat
     1081 tgccacccgc cgccagcgag gccgaggcgc tcgccgagtt gcgggcgctg gccgattcca
     1141 acaccgtggc ggtgtcgatg atcgggcagg gctattacga cacgctcaca ccgccggtgt
     1201 tgctgcgcaa tatcatggag aaccccgcct ggtacaccgc ctacacgccc taccagccgg
     1261 agatcagcca gggccggctg gaagcactgc tgaacttcca gaccatggtg gccgacctca
     1321 ccggcctcga agtcgccaac gcgtcgatgc tcgacgaggg caccgccgcc gccgaggcca
     1381 tgacgctgat gcaccgcgca gctcgcggca cgtcaaaacg gctcgccgtg gacggcgatg
     1441 tcttcgccca gactgcagcc attctggcca cccgggccaa gccgttgggc atcgagatcg
     1501 tcaccgccga cctgcgtgac ggcctgccgg acggcgagtt cttcggcgtc atcacgcagc
     1561 tgccgggcgc aagcggccgg atcaccgact ggtccgcttt ggtggcgcag gcgcacgacc
     1621 gtggcgcact ggtggccatc ggcgccgacc tgttggcgct gacgctgctc acgccgcccg
     1681 gcgagatcgc tgccgacgtc gcgtttggta ccacccaaag gttcggtgtg ccaatgggat
     1741 tcggtgggcc gcacgccggc tacctcgcgg tgcacaccaa gcacgcccgt cagctgccgg
     1801 gacggctggt cggagtgtcg ctggatgccg acggttcgcc ggcctaccgg ctggcgctgc
     1861 agacccgcga gcagcacatc cgccgcgaca aggcgaccag caacatctgc accgcgcagg
     1921 tgttgctggc ggtgatggcc gcgatgtacg ccagctatca cggctcggac ggtctcaccg
     1981 agatcgctcg ccgggtgcac tcccacgccg cgaccatcgc cgccgcgctg ggcgacgcgc
     2041 tggcacacga cgcgttcttc gacacggtgt tggcccgggt gccggggcga gcggacgagg
     2101 tggtggccgc ggccaaggcc aacgggatca acctgtggcg cgtcgacgcc gatcacgtgt
     2161 cggtggcctg cgacgaggcc acgaccggcg cccacgtggc ggctgtcctg gacgccttcg
     2221 gggtggcttc cacttttgcg gcccgcgagc cggccgctgc cgacatcgcg acccgcacgt
     2281 cggatttcct gacacatccc gcattcaccc aataccgcac cgagacggcg atgatgcgct
     2341 acctgcgcac gttggcggac aaggatatcg ccttggaccg cagcatgatt ccgctcggct
     2401 cgtgcacgat gaaactcaac gccgccgcgg aaatggaatc ggtcacctgg ccggaattcg
     2461 cccgccagca cccgttcgcc ccggcgtccg acatccctgg actgcgtcgc ctcatcgccg
     2521 atctggagag ctggctggtg gccatcaccg gctacgacgc tgtctcgctg caacccaacg
     2581 ccggatcgca aggcgagtac gccggcctgt tggcgatcca cgactaccac gccagccgcg
     2641 gcgagccgca ccgcaccatc tgcctgatcc cgtccagcgc gcacggcacc aacgcggcat
     2701 cggctgcgtt ggccggcatg cgggtggtcg tggtggcctg ccacgataac ggcgatgtcg
     2761 acctggacga cctgcgcgcc aaggtcggtg aacacaccga gaagctgtca acgctgatga
     2821 tcacctaccc gtccacccac ggcgtgtacg agcacgacat cgccgacatc tgcgcggccg
     2881 tgcatgacgc cggcggccag gtgtacgtcg acggggccaa cctcaacgcg ctggtcggcc
     2941 tggcccggcc ggggaagttc ggcggggacg tcagccacct gaacctgcac aagacgttct
     3001 gcatcccgca cggcggcggc ggcccgggcg tcggtccggt ggcggtccgt tcgcatctgg
     3061 caccattcct gcccggacac ccgtatgcac ccgaactgcc ggagggtcat ccggtgtcgt
     3121 cggcgccgta cggctcggcg tcgatcctgc cgatcacctg ggcctacatc aggatgatgg
     3181 gcgccgacgg cctgcgggcc gcctcgctga ccgcgatcac ctcggccaat tacattgccc
     3241 gacgcctcga cgagtacttc ccggtgctgt acgccggtga caacggcatg gtcgcccacg
     3301 agtgcatcct ggacctgcgc ggtatcacca agtccaccgg agtgacggtc gacgatgtcg
     3361 caaaacgcct ggcggactac gggtttcacg cgcccaccat gagcttcccg gtggccggca
     3421 cgctgatggt ggaacccacc gagagtgaga cgctgaccga ggtcgacgcg ttctgcgagg
     3481 cgatgatcgc catccgcagc gagatcgacc gcgtcggcgc gggggagtgg ccggttgacg
     3541 acaatccgct ccggggggcg ccacacaccg ctgagtgcct ggtggtctcc cactgggatc
     3601 atccctatac ccgcgaagag gcggcctatc cgctcggcaa ggccttccgg cccaaggtgt
     3661 ggccgccggt gcgccgcatc gacggcgcct acggcgaccg caacctggtc tgctcgtgcc
     3721 cgccggtgga agccttcgcc tgaggcgtcc cgcagtccgt cagccgaagc gctcgacgat
     3781 cgccgcggcg atctggccgg gttcgtcctc ctggatgaag tgcttggcct cgggcagttc
     3841 gacgaggacg tgatccggaa acgccgcccg catccgcggg agcgtcgggc cgggccgaaa
     3901 cgcgaagtcc ttcatgcccc acaccaacag ggcgggcttg gcacccagct tggccggcac
     3961 ttcgcggccg agccgcgtca gcaggggatg ggcggccaaa atctgcttgg gcatctcggc
     4021 cacgcccttg cgcgtgctcg ggcttggctg cacggctcgg tagtgctcca tcaccacggc
     4081 actgagccgg tgtgccgttc ctagcgggat caggtgctcc acgaagtaat tgcgctgcag
     4141 aatcgcccac tgcatggggg gactcgacat cacaaggctg aagatcttcg tcgtcagctc
     4201 gtccgctcgc cagaaccagg tgttgcccaa gatgacgccg cgcacccggt cggcgcgctc
     4261 cacatcgacc gccatgctga tcggcccacc ccagtcctgg cccatcgtca ggtagtcgtc
     4321 caggccgaga tggtcgacga attcgccgat cacctt
//
LOCUS       DASSGQ010000118         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_495140,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000118 DASSGQ010000000
VERSION     DASSGQ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495140"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..410
                     /locus_tag="VFI55_05580"
     CDS             96..410
                     /locus_tag="VFI55_05580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HET7073537.1"
                     /translation="MPVVVVATLTVKPESVDTVRDILTRAVEEVHDEPGCQLYSLHQT
                     GDTFVFVEQWADPEALKTHSGAPAITKMFAAAGEHLAGAPDIKMLQPVPAGDPDKGQL
                     RA"
     gene            410..1231
                     /locus_tag="VFI55_05585"
     CDS             410..1231
                     /locus_tag="VFI55_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HET7073538.1"
                     /translation="MGRPLNGKVALITGAARGQGRAHAVRLAADGAGVIAVDLCEQIA
                     SVPYPLATPDDLAATVKLVEDTGSRIVARQADVRDRASLAAALQAGLDEFGRLDIVVA
                     NAGIAPMQSGPDGWRDVIDVNLTGVFHTVEAAIPTMVSQGSGGSIVLISSAAGLAGVG
                     SADAGSIGYAAAKHGVVGLMRVYANLLARHSIRVNSIHPSGVDTPMINNEFTRQWLAR
                     LVAESDSPPDMGNALPVQVMQSDDIANAVAWLVSDQARYITGVTLPVDAGFLNKK"
     gene            1235..>2024
                     /locus_tag="VFI55_05590"
     CDS             1235..>2024
                     /locus_tag="VFI55_05590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET7073539.1"
                     /translation="MARNPVAQTAFGPMVLAAVEQNEPPGRRLVDDDLAELFLPAPMR
                     GLVAATRSVPVRNLMIRASEWSGRGLWASLACRKRFIADKLEEAIGDIDSVVILGAGL
                     DTRAYLLTRQVRIPIFEVDLPVNIARKAKTVRRVLGGAPLSVRLVALDFERDDLLTSL
                     TEHGYHTDYRTFFICEGLTQYLSEKTVRRTLGGLRAAAPGSRLSFTYVRRDFIDGTNR
                     YGTRTLYRDVRQRHQLWHFGLQPDEVAEFLAQYGWRLVEQPDPNE"
BASE COUNT          345 a          700 c          657 g          322 t
ORIGIN      
        1 gtgaccgatc gatcgcaacc gagcgtgagt agggtggcaa cgggtgacga acgtcacgcc
       61 gtcacccatc cccggagttc ccaggaggcc ctaacatgcc cgtcgtcgtc gtcgccaccc
      121 tgaccgtcaa acccgaatcg gtcgacaccg tccgtgacat cctcactcgc gcggtcgagg
      181 aagtgcacga cgaaccgggc tgccagctgt actcgctgca tcaaacgggc gacaccttcg
      241 tcttcgtcga gcaatgggcc gaccccgaag cgctcaagac ccacagcggc gccccggcga
      301 tcaccaagat gttcgcggcg gccggcgagc acctggccgg ggccccggac atcaagatgc
      361 tgcagccggt tcccgcgggc gatccggaca agggacagct gcgcgcctaa tgggccggcc
      421 cctgaacggc aaagtcgcgc tcatcaccgg cgccgcgcgc ggccagggcc gcgcacacgc
      481 ggtgcgactg gccgccgacg gcgccggcgt gatcgcggtc gacctgtgcg agcagatcgc
      541 cagcgtgccc tatccactgg ccacgcccga cgacctggcg gccacggtca agctcgtcga
      601 ggacaccggc tcgcgcatcg tggcaagaca ggccgacgtg cgcgaccgcg cgtcgctggc
      661 ggccgcgttg caggcgggcc tcgacgagtt cggccgcctg gacatcgtcg tggccaacgc
      721 cggtatcgcg cccatgcagt cgggtcccga cggctggcgc gacgtcatcg acgtcaacct
      781 gaccggcgtg ttccacaccg tggaggcggc gataccgacc atggtctcgc aaggcagtgg
      841 cggctcgatc gtgctgatca gttcggccgc gggactggcc ggcgtcggca gcgccgacgc
      901 cggctccatc ggctatgccg ccgccaaaca cggggtggtc gggttgatgc gcgtgtatgc
      961 gaatctgctc gcgcgacaca gcatccgggt caactcgata catccgtccg gtgtcgacac
     1021 cccgatgatc aacaacgagt tcacccggca gtggctcgcc cggttggtcg ccgagtccga
     1081 ttcaccgccc gacatgggca acgcgctgcc ggtacaggtt atgcaatccg atgacatcgc
     1141 caacgcggtg gcatggctgg tgtccgacca ggctcgctac atcaccggcg tcaccttgcc
     1201 ggtcgatgcg ggctttctga acaagaaata gctcatggca cgaaatcccg ttgcacagac
     1261 cgcctttggc ccgatggtgt tggccgccgt cgaacaaaac gagccacccg ggcgccgcct
     1321 ggtggacgac gacctcgcgg agctgttcct gccggccccg atgcgagggc tggtcgccgc
     1381 cacccgttcg gtaccggttc gcaacctgat gatccgcgcg tcggagtggt cgggccgcgg
     1441 gctgtgggcc agcctggctt gccgcaagcg tttcatcgcc gacaagctcg aagaagcgat
     1501 cggagacatc gactcggtcg tcatcctcgg tgcgggattg gacacccgtg cgtacctgct
     1561 gacacgccaa gtccgcatcc cgatcttcga ggtagacctg ccggtcaaca tcgcccgcaa
     1621 ggccaagacc gtccggcggg tgctgggtgg ggcgccgctg tcggttcggt tggtggcgtt
     1681 ggacttcgag cgcgacgact tgctgacctc gttgaccgag cacggctatc acaccgatta
     1741 ccgaaccttc ttcatctgcg agggcctgac ccaatacctg tccgagaaaa ccgtccggcg
     1801 gacgttgggc ggattgcggg cggccgcccc cggcagccgg ctgtcgttca cctacgtccg
     1861 cagggatttc atcgacggga cgaatcggta cggcacccgc acgctgtacc gcgatgttcg
     1921 ccagcgccat caactgtggc acttcggctt acagcccgac gaggtcgcgg agttcctcgc
     1981 ccaatacggc tggcggctgg tggagcaacc cgatcccaac gaac
//
LOCUS       DASSGQ010000119         3960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_191277,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000119 DASSGQ010000000
VERSION     DASSGQ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191277"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VFI55_05595"
     CDS             <1..801
                     /locus_tag="VFI55_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET7073540.1"
                     /translation="TMEQIAGLAWVTGLPDAPPVAPRGACDPLAGVHAAFAVLAALDF
                     AERTGSGQLVELPMLESVLNVTAIQAIESEVFGVTLGRRGNRGHGESIQNLYRCAGSD
                     DDWIAVTVRDDRQWHALVELMDRPHWCDESLATVGGRRERADEIDGRLAAWFADQPLD
                     AAVRRLAGAGIPAAPVVSPSLVTENLQLRDRGFFEALHHPSTGAGLYPCPPFARLAGQ
                     DRWLRRPPPKLGEHNGEVLRDRCGLTEEELVNLAGAGVIGTRPTGLGK"
     gene            860..1051
                     /locus_tag="VFI55_05600"
     CDS             860..1051
                     /locus_tag="VFI55_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014981412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET7073541.1"
                     /translation="MRVTVDENLCEANGFCESLAPDVFELGDADVVQIADGPVPPRLE
                     IDVRAAVDQCPKAALRLQG"
     gene            1199..1624
                     /locus_tag="VFI55_05605"
     CDS             1199..1624
                     /locus_tag="VFI55_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005241262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7073542.1"
                     /translation="MAKYAAMRPYFEVVAEGLEGLVDGTDFFDMHAEDVVVEYVITVP
                     DYPRRIEGREALAELYADYGDSIVQSGSSDVYRYYDPEKSVVVLEYTMHGTVVGTGAR
                     YVNRFISVITIADRKIVHWRDYLDPLAVFAAFGDSPAPY"
     gene            complement(1626..2876)
                     /locus_tag="VFI55_05610"
     CDS             complement(1626..2876)
                     /locus_tag="VFI55_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7073543.1"
                     /translation="MTAAAVDLSDSALWRNGFPDEMFTELRSTRPLFRHDLTPGVTRL
                     FSGSVRREFWVTTKHRHAVRLHRDAESFTAVDGPLIQPVGMFSSYPTIINMDPPGLNR
                     RRKLISNAFNPRAVAKLEEGIRSRAARMIDKLLADDGGDWISDVADALPMTVIGDIIG
                     IPESDRPRIFDCLDRILKARSPEAGLSRQQEIDLYANIFGYAMELTADKRRTPADDIW
                     STLASAVIVGEDGAQFSLPTNELEFFFFVLAFAGSDTTKNALAAGLQAFVENPEQIER
                     YRAQEALRPSAVEEVLRWASPVAYWTRTAKVDTEMDGQCIAQGERVVSMLRSANRDEE
                     VFSSPFSFDIGRQPNPQVAFGGGGPHHCLGATLARTELRAVFDELLLRCDDIALGPAK
                     AAHPNLLTNMSIYDAMSIQLTERR"
     gene            3040..>3960
                     /locus_tag="VFI55_05615"
     CDS             3040..>3960
                     /locus_tag="VFI55_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET7073544.1"
                     /translation="MNHTVSESAPDSSGDASEREFGPSGIALSTYRFPTGWFIVAFAS
                     DLPAGEVKRAHYFGQELVLFRTESGQVHVMEAYCQHLGANLGVGGTVEGENIVCPWHG
                     WRWRGDGTNALIPYSKIGCKTNVRIRTYPSVQWYGFILAWHERHGRAPYWQPPVLPEL
                     ETNEYYPLHPHTEMLNRVKVHPQMIIENAADPYHVQYVHKAASPATTASFETSGYHLH
                     ATVNAHFGGGRASTWLTPQGPVDAKIVYDNYSLGLGVVRFPSELVATVQVTGQTPVDE
                     DYTDYFYTQASIREPGDTGDAPTGRAAKFLA"
BASE COUNT          690 a         1254 c         1331 g          685 t
ORIGIN      
        1 accatggagc agatcgcggg cctggcctgg gtgaccgggc tgccggacgc gccgccggtg
       61 gctccgcggg gagcctgtga tccgctggcg ggagtgcacg ccgcgttcgc cgttctggcc
      121 gcgttggact tcgccgaacg caccggatcg ggccaattgg tcgagctgcc gatgctggaa
      181 tcggtcctga acgtgaccgc gatccaggcg atcgaatccg aggtcttcgg cgtgactctg
      241 ggccgtcggg gcaaccgcgg tcacggtgag tcgatccaga acctgtaccg gtgcgcgggc
      301 tcggacgacg actggatcgc ggtcaccgtg cgcgacgacc ggcagtggca tgcgctggtc
      361 gagctgatgg atcgaccgca ctggtgtgac gagagcctgg ccaccgtggg tgggcggcgc
      421 gagcgagccg acgagatcga tggccgactg gcggcttggt tcgccgacca accgctggat
      481 gcggcggtgc ggcggttggc cggcgcgggt attcccgctg cccccgtggt atcgccgtca
      541 ctggtgaccg agaatcttca actgcgcgat cgcgggttct tcgaagccct gcaccacccg
      601 agcaccggcg ccggcctgta cccctgtccg ccattcgcca ggttggccgg gcaggacagg
      661 tggctgcgac ggccgccacc caagctcggc gagcacaatg gggaggtatt gcgtgatcgg
      721 tgcgggctga ccgaagagga actggtgaac ctggcgggcg ccggagtgat cgggacccgg
      781 ccgacgggcc tcggcaaata gcgggctttt gaaaaaatgc ggcctggaag ggcctcaaaa
      841 tgggtgagga ggtggtctga tgcgcgtgac ggtcgacgag aacttgtgtg aggcaaacgg
      901 tttctgcgag tcactcgctc cggacgtatt cgaactgggc gacgccgacg tggtccagat
      961 cgccgacggc ccggtgccgc cgcgcctgga gatcgacgtt cgagccgcgg tggaccagtg
     1021 cccgaaggcc gcgctgcggc tacagggctg atcagctcat cgctgcgcgg tcagccaccg
     1081 gctgcggtgt ccgtggcgtc ggcagtgcag gcggccgagg atgtgtcggt accgcggttt
     1141 agtgtcgacg cagaccaatg cagccgatga atggggggcg tccatggcgt cgcgggacat
     1201 ggcgaaatac gctgcgatgc ggccgtactt cgaggttgtc gcggagggcc tcgagggttt
     1261 ggtcgatggg accgattttt tcgatatgca cgccgaggac gttgtggtgg agtacgtcat
     1321 caccgtcccg gactacccgc gcagaatcga agggcgagaa gcgctggccg aactctacgc
     1381 cgattacggc gattcgatcg tccagagtgg cagcagcgac gtgtatcgct attacgaccc
     1441 cgagaaatcc gtcgtggtgc tcgagtacac gatgcacggc acggtagtgg gcacgggcgc
     1501 gcggtatgtc aaccgcttca tctcggtgat caccatcgcg gaccgcaaga ttgtgcactg
     1561 gcgcgactat ctggatcctt tggcggtatt cgccgcattc ggcgactcgc cggcgcccta
     1621 ctgacctatc gccgctccgt cagctgtatc gacatcgcgt catagatcga catgttggtg
     1681 agcaggttcg gatgagccgc tttggccgga ccgagcgcga tgtcatcgca tcggagcaac
     1741 agttcgtcga acaccgcgcg gagttccgtg cgcgccagcg tggcgcccag gcagtgatgc
     1801 ggtccgccgc ctccgaaggc cacctgcggg ttgggctgac ggccgatgtc gaagctgaac
     1861 ggcgaggaga acacctcctc gtcgcggttc gccgaacgca gcatcgacac cactcgttca
     1921 ccttgggcga tgcactgacc atccatctcg gtatcgacct tcgcggtccg tgtccagtac
     1981 gccaccggtg atgcccagcg caacacctcc tcgacggcgc tggggcgcaa tgcttcctgc
     2041 gctcggtaac gctcgatctg ctccggattc tcgacgaacg cctgcagtcc ggcggccaac
     2101 gcattcttgg tggtgtcact gccggcgaac gccagcacaa agaaaaagaa ctcgagttcg
     2161 ttggtgggca ggctgaattg cgcaccatct tcgccgacga tcaccgcgga cgcgagtgtg
     2221 ctccagatgt cgtcggcggg agtgcgtcgc ttgtctgcgg tgagctccat tgcgtagcca
     2281 aagatgttgg cgtacagatc gatttcctgc tgccgactca gccccgcctc gggtgagcgg
     2341 gccttcaaga tgcggtccag gcaatcgaag atccgcggcc ggtcggattc cggtatgccg
     2401 atgatgtcgc cgatgaccgt catcggcagg gcgtcggcaa cgtcggagat ccagtcgccg
     2461 ccgtcgtcgg cgagcagctt gtcgatcatc cgcgccgcac gcgaccggat gccttcctcg
     2521 agtttggcga ccgcacgcgg gttgaaggcg ttggagatga gcttgcgccg tctgttcagg
     2581 cccggcgggt ccatgttgat gatcgtcgga tacgacgaga acattccgac cggctggatg
     2641 aggggtccgt cgaccgcggt gaaggattcg gcgtcgcggt gcagccggac cgcgtgccgg
     2701 tgcttggtgg tcacccagaa ttcgcggcgg acggatccgg aaaataaccg agtcacaccg
     2761 ggtgtcaggt cgtgcctgaa cagcggcctg gtgctgcgca gctcggtgaa catctcgtcg
     2821 ggaaagccgt tgcgccacaa ggcggaatcg gagaggtcca ctgccgcagc cgtcatcgaa
     2881 agccccgctc tgcccgaaca ccacgtcgga gccggcggcg ccgcgttgac gttcaatacc
     2941 taaaatagta aaaatagacc tagtcgtcga accgcggtga atcgagggcg gcccagatgc
     3001 ggtcggctca ccttggtcca ggcgggatac ggagtgctgg tgaaccacac agtttcggag
     3061 tcggcgccgg attcgtccgg agatgcatcg gaacgtgagt tcggaccgtc cggcatcgcg
     3121 ctgtcgacct accggttccc gacgggatgg ttcatcgtcg ccttcgcatc cgacctaccg
     3181 gccggtgagg tgaagcgcgc gcactacttc ggtcaggagt tggtgctgtt ccgcaccgag
     3241 tccggccagg tgcacgtcat ggaggcctat tgtcagcacc tcggcgccaa cctgggcgtg
     3301 ggcggaacgg tggaaggcga aaacatcgtc tgcccctggc acggctggcg ctggcgcggt
     3361 gacggcacca acgcgctgat tccgtacagc aagatcggct gcaagaccaa cgtccggatc
     3421 cgcacctatc ccagcgtcca gtggtacggc ttcatcctcg cctggcacga acggcatggc
     3481 cgtgcgccct actggcagcc accggtgctg cccgagctgg aaaccaacga gtactacccg
     3541 ctgcacccgc acaccgagat gctcaaccgg gtcaaggtgc acccgcaaat gatcatcgag
     3601 aacgccgccg acccctacca cgtccaatac gtgcacaagg ccgccagccc cgccaccacg
     3661 gcatcgttcg agacatccgg ctaccacctg cacgccaccg tcaacgccca tttcggcggt
     3721 ggccgcgcgt cgacctggtt gaccccccag gggcccgtcg acgccaagat cgtctacgac
     3781 aactactcgc tcgggctggg ggtcgtccgc ttcccgagcg aactggtggc caccgtccag
     3841 gtcaccgggc agaccccggt cgacgaggac tacacggact acttctacac ccaggcctcc
     3901 atccgcgagc ccggcgacac cggtgacgcg cccaccggcc gcgccgcgaa attcctggcg
//
LOCUS       DASSGQ010000120         7392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1193623,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000120 DASSGQ010000000
VERSION     DASSGQ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7392
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193623"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..718
                     /gene="hisB"
                     /locus_tag="VFI55_05620"
     CDS             86..718
                     /gene="hisB"
                     /locus_tag="VFI55_05620"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HET7073545.1"
                     /translation="MTATEPRLATRRARIERRTKESDIVIDLDLDGTGQVDIDTGVPF
                     YDHMLTALGTHASFDLTVRTKGDVEIEGHHTIEDTAIALGQALGQALGDKKGIRRFGD
                     AFIPMDETLAHAAVDVSGRPYCVHTGEPDHLQHTTIAGSSVPYHTVINRHVFESLAMN
                     ARIALHVRVLYGRDPHHITEAQYKAVARALRQAVEPDPRVSGVPSTKGVL"
     gene            715..1335
                     /gene="hisH"
                     /locus_tag="VFI55_05625"
     CDS             715..1335
                     /gene="hisH"
                     /locus_tag="VFI55_05625"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HET7073546.1"
                     /translation="MTGKSVVILDYGSGNLRSAQRALQRVGASVEVTADADAAAAADG
                     LVVPGVGAFEACMTGLRKISGERIIAERAAAGRPVLGVCVGMQILFARGVEFGVETTG
                     CGLWPGAVTRLDAPVIPHMGWNVVDSGPGSVLFQGLDDNARFYFVHSYAAQRWEGSSA
                     AVLTWATHQVPFLAAVEDGPLAATQFHPEKSGDAGAAVLSNWVEGL"
     gene            1338..2093
                     /gene="priA"
                     /locus_tag="VFI55_05630"
     CDS             1338..2093
                     /gene="priA"
                     /locus_tag="VFI55_05630"
                     /EC_number="5.3.1.16"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003407955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase/phosphoribosylanthranilate isomerase
                     PriA"
                     /protein_id="HET7073547.1"
                     /translation="MPLILLPAVDVVDGRAVRLVQGKAGSETEYGSALDAALGWQRDG
                     AEWIHLVDLDAAFGRGSNRELLAELVGKLDVHVELSGGIRDDDSLAAALATGCARVNL
                     GTAALENPQWCARAISEHGDKVAVGLDVQIDPGNPGGEHRLRGRGWETDGGDLWDVLE
                     RLDSQGCSRYVVTDVTKDGTLGGPNLDLLARVADRTEAPVIASGGVSSLDDLRALANL
                     ATLTGRGVEGAIVGKALYAERFTLPQALAAVKA"
     gene            2099..2911
                     /locus_tag="VFI55_05635"
     CDS             2099..2911
                     /locus_tag="VFI55_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET7073548.1"
                     /translation="MDLNALVSPLVDQASAVLDAAVPRFLAGHRADSAVTKKGNDFAT
                     EVDLAIERQVVAALEAATGIGVHGEEFGGTDVDSAWVWVLDPVDGTFNYAAGSPMAAI
                     LLALLHQGEPVAGLTWLPFTDERYTAVAGGPLLKNGKPQPTLRPAELAESLVGVGTFS
                     ADSRGRFPGRYRLAVLEDLSRVCSRLRMHGSTGIDLVYVADGILGAAITFGGHVWDHA
                     AGVAQVRSAGGIVTDLAGKPWTPASRSVLAAAPGVHGEVLEILCAVGKPEDY"
     gene            2913..3698
                     /gene="hisF"
                     /locus_tag="VFI55_05640"
     CDS             2913..3698
                     /gene="hisF"
                     /locus_tag="VFI55_05640"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HET7073549.1"
                     /translation="MYADTGLAVRVIPCLDVDAGRVVKGVNFENLRDAGDPVELAAAY
                     DAEGADELTFLDVTASSSGRATMLEVVRRTAEQVFIPLTVGGGVRTVADVDTLLRAGA
                     DKVSVNTAAIARPELLGEMATQFGSQCIVLSVDARTVPAGSAPTPSGWEVTTHGGRRG
                     TGIDAVEWAARGADLGVGEILLNSMDADGTKAGFDLPMLRAIRAAVTVPVIASGGAGA
                     AEHFAPAVAAGADAVLAASVFHFRELTIGQVKAAMAAEGITVR"
     gene            3695..4042
                     /gene="hisI"
                     /locus_tag="VFI55_05645"
     CDS             3695..4042
                     /gene="hisI"
                     /locus_tag="VFI55_05645"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HET7073550.1"
                     /translation="MTLDPNIAARLKRNAEGLVAAVVQERGSGDVLMVAWMDDEALAR
                     TLKTREATYYSRSRAQQWIKGATSGHTQHVHSVRLDCDGDTVLLTVDQVGGACHTGDH
                     SCFDADVLLQPER"
     gene            complement(4048..5592)
                     /locus_tag="VFI55_05650"
     CDS             complement(4048..5592)
                     /locus_tag="VFI55_05650"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET7073551.1"
                     /translation="MPETDGLKEGQVFAGYTIIRRLGTGGMGQVYLAQHPRLPRRDAL
                     KVLPGELTANDEFRQRFNREADLAASLYHPHIVGIHDRGEYQGQLWISMDYVEGTDAA
                     KLLRTRFPSGMPKADVVEIISAVADALDYAHSRGLLHRDIKPSNILLTEANPRRRILL
                     ADFGIARELGDISGLTATNMLVGTTAYCAPEQLQGADLDGRADQYALGCTAFDLLAGS
                     APFDHSNPAVVITQHLSAPPPHISTRRPELASLDGAIAKALAKKPEDRYPTCADFAAA
                     LGSQIDTAAAHRAAAPTAISAAPMTLSAAPTEVIAAPASTPSASPKARGSWRGPAIAV
                     AALVLVALLAVSAYAGVRMLDARAGQHNGGSSPSQLVAPPPGQAVPPPSTPAIPLSSQ
                     ITDQSGVLGPLEHDAVNRALTKLYNGQGTRLWVVYVKNFNGLKPLRWTEDTMRANNFT
                     DSDALLAVATDQPAFSFRVPNAVIAGRAIDLDTIRRDRIQPAVSRHEWARAAIVAANG
                     LDVAPS"
     gene            5697..6797
                     /locus_tag="VFI55_05655"
     CDS             5697..6797
                     /locus_tag="VFI55_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium:proton antiporter"
                     /protein_id="HET7073552.1"
                     /translation="MLKRASWSSWTVVVPVLAVVTLVAAWGRHLSPVLIAVEATFLIG
                     AVLAAVHHAEVVAHRVGEPYGSLVLAAAVTIIEVALILALMASGGDEAATLPRDTVFA
                     AVMITMNGIAGVSLLLGSRRYGVTTFNAEGSGAALATLTTLATLSLVLPTFTTSHGGL
                     EFSPGQLIFAAVASLGLYLLFVFMQTVRHRDFFLPVAQKGQKGLFEDETHADPPSTSA
                     ALRSLGVLLLALVAVVGLAEQESPAIEHVVSAAGFPQSFVGVVIASLVLLPETLAAAR
                     AARQGRIQTSLNLAYGSSLASIGLTIPTIALSEIWLKGPLLLGLAPTQIVLFALTVVI
                     SVLTVVPGRATRLQGEVHLVLLAAYVFLAIVP"
     gene            6990..>7392
                     /locus_tag="VFI55_05660"
     CDS             6990..>7392
                     /locus_tag="VFI55_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073553.1"
                     /translation="MAAKKPEDKPDIPINAELARMDAEGVDVADVIALAGYVGRSPKE
                     GVVRLHPGLQDLSISIDIAASDIVATREAPAATMPLGGVVLWVSGSADVTFRRTRTVV
                     AAAQEVRGFFGAGPALEPSSNDPVDRLNIQVR"
BASE COUNT         1146 a         2362 c         2617 g         1267 t
ORIGIN      
        1 tggccgccga gaacgacggg ttccttgcgg caagcgcccg aatcgcggcc accgaattgg
       61 ccccagccac ccccgtagga gcctcgtgac agccaccgaa ccacgccttg caacgcgtcg
      121 cgcgcgcatc gagcgtcgca ccaaggaatc cgacatcgtc atcgatctcg acctggacgg
      181 caccgggcag gtcgacatcg acaccggggt gccgttctac gaccacatgc tgacggcgct
      241 gggcactcac gccagcttcg atctgaccgt gcgcaccaag ggtgatgtgg agatcgaagg
      301 gcaccacacc atcgaggaca ccgccatcgc gctgggtcag gcgctcgggc aggccctggg
      361 cgacaagaag ggcatccgcc ggttcgggga cgccttcatc ccgatggacg agacgctggc
      421 ccacgccgcg gtcgacgtgt cgggccggcc ctactgcgtg cacaccgggg agccggatca
      481 cttgcagcac accacgatcg cgggcagctc ggtgccttac cacaccgtga tcaaccgcca
      541 tgtgttcgag tcgctggcga tgaacgcccg catcgcgctg cacgtgcgcg tcctgtacgg
      601 gcgcgacccg caccacatca ccgaagccca gtacaaggcc gtggcccgcg cgttgcgtca
      661 ggcggtcgaa cccgatccgc gtgtatcggg cgtgccgtcc accaaaggtg ttctgtgacg
      721 gggaaatcgg ttgtaattct agattacggc tccggtaacc tgcggtcggc ccagcgcgcg
      781 ctgcagcggg tcggcgcctc cgtcgaggtg accgccgatg ccgacgccgc cgcggccgcc
      841 gacggtctgg tggtgcccgg cgtcggcgcg ttcgaggcgt gcatgacggg cctgcgcaag
      901 atctcggggg agcggatcat cgccgagcgg gccgccgccg gacgcccggt gctgggcgtc
      961 tgcgtcggca tgcagatctt gttcgcgcgc ggtgtcgagt tcggtgtgga gaccacgggt
     1021 tgcgggctct ggccgggcgc cgtcacccgc ctggatgccc cggtgatccc gcacatgggc
     1081 tggaacgtgg tggattccgg gccgggcagc gtgctgttcc aagggctgga cgataatgcc
     1141 cggttctact tcgtgcactc ctacgccgcg cagcgctggg aagggtcctc ggcggcggtg
     1201 ctgacgtggg cgacccacca ggtgccgttc ttggccgcgg tcgaggacgg cccgctggcc
     1261 gcaacccaat tccatcccga aaagagcggc gacgcgggcg cggccgtgct gagcaactgg
     1321 gtcgagggac tgtgaaaatg ccgctgatct tattaccggc cgtcgacgtg gtcgacggtc
     1381 gcgccgtgcg cctcgtccaa ggcaaggccg gcagtgaaac cgagtacggc tcagcgctgg
     1441 acgccgccct gggctggcag cgcgacgggg ccgagtggat ccatctggtc gacctggacg
     1501 ccgcgttcgg tcgcggctcc aaccgcgaac tgctcgccga gctggtgggc aagcttgacg
     1561 tgcacgtgga gctgtccggc gggatccgcg acgacgattc gctggccgcg gcgttggcca
     1621 ccggttgcgc gcgggtcaac ctgggaacgg ctgccctcga gaacccgcag tggtgtgcga
     1681 gggcgatcag cgagcacggc gacaaggtcg ccgtcgggtt ggatgttcag atcgatccgg
     1741 gaaatccagg cggcgagcac cggctgcgcg gacgcggctg ggaaaccgac ggtggcgatc
     1801 tgtgggacgt gctggaacgc cttgatagtc aaggatgttc gcggtacgtg gtgaccgacg
     1861 tcaccaagga cggcacattg ggcgggccca acctcgacct gctggcccgg gtggccgatc
     1921 gcaccgaagc accggtgatc gcatccggcg gagtatccag cctcgatgac ctgcgcgcct
     1981 tggcaaacct cgcgaccctg accggccgcg gggtcgaggg cgccatcgtc ggcaaggccc
     2041 tctatgcgga gcggttcacc ctgccgcagg cgctggccgc ggtaaaggcg taggcgcaat
     2101 ggatctcaac gcgctggtgt cccctttggt tgaccaggca tcagcggtgc tcgacgccgc
     2161 cgtgccgcga ttcctggcgg gtcaccgcgc cgattcggcg gtaaccaaga agggcaacga
     2221 tttcgccacc gaagtcgacc tcgccatcga gcgtcaagtc gttgccgcgt tagaagcggc
     2281 caccggaatc ggcgtgcacg gtgaggaatt cggcggcacg gacgtcgact cggcctgggt
     2341 gtgggtgttg gatcccgtgg acggcacgtt caactatgcc gccggatctc cgatggccgc
     2401 gatcctgctg gcgctgctcc accagggcga acccgtggca ggccttacct ggttgccgtt
     2461 caccgacgag cgctacaccg ccgtcgcggg cggcccgctg ctgaaaaacg gcaaaccgca
     2521 accaacgctg cgtcccgcgg agctggccga aagcctggtc ggggtcggaa cattcagcgc
     2581 tgactcgcgc ggccgattcc cgggacgcta tcggctcgcg gtgctggaag atctcagccg
     2641 ggtgtgttcg cgactgcgca tgcacggctc caccgggatt gacctggtct acgttgccga
     2701 tggaatcctg ggtgccgcaa tcactttcgg tggccatgtc tgggaccacg ccgccggggt
     2761 ggcgcaggtc agatccgcgg gtggcattgt caccgacctg gccggcaaac cgtggacccc
     2821 ggcatcgcgg tcggtgctcg ccgcggcgcc cggcgtacac ggcgaggtcc tcgagatcct
     2881 gtgtgccgta ggtaaaccgg aggattactg agatgtacgc cgacaccggc cttgccgtgc
     2941 gggtgatccc gtgcctggac gtcgatgccg ggcgggtggt caagggggtc aacttcgaaa
     3001 accttcggga cgcaggcgat ccggtggaat tggccgccgc ctacgacgcc gagggcgccg
     3061 acgagctgac ctttctggac gtgaccgcgt cgtcgtcggg ccgggcaacg atgctggagg
     3121 tggtgcgccg caccgccgag caggtgttca tcccgctgac ggtcggtggc ggcgtgcgca
     3181 cggtggccga cgtcgacaca ctgctgcggg caggcgccga caaggtgtcg gtcaataccg
     3241 ccgccatcgc gcgccccgag cttttggggg aaatggcaac gcaattcggt tcccagtgca
     3301 tcgtgttgtc cgttgacgca cgcaccgtgc ccgcggggtc cgcgccgaca ccgtcgggct
     3361 gggaggtgac gacccatgga ggtcgccgcg gcaccggtat cgatgccgtc gagtgggcgg
     3421 ctcgcggcgc cgatctcggg gtgggcgaga tcctgctgaa ctcgatggac gccgacggga
     3481 ccaaagccgg gttcgacctg ccgatgctgc gtgcgatccg cgcggcggtc acggtgccgg
     3541 tgatcgccag cggtggggcc ggcgccgccg aacatttcgc ccccgcggtc gccgccggtg
     3601 cggatgcggt gttggcggcc agcgtttttc acttccgcga gctgacgatc gggcaggtga
     3661 aggccgcgat ggccgccgaa gggatcacgg tgcgatgacg ctcgacccga acatcgccgc
     3721 gcgcctgaag cgcaatgccg aaggactggt cgcggccgtt gtgcaggagc gtggcagcgg
     3781 cgacgtgctg atggtcgcct ggatggacga cgaggcgctg gcccgcaccc tgaaaacccg
     3841 tgaggcgacg tactattcgc gctcccgggc ccaacagtgg atcaagggcg cgacgtccgg
     3901 ccacacgcag cacgtccact cggtacggct ggactgcgac ggcgacaccg tgctgctgac
     3961 ggtcgaccag gttggcggcg cgtgccatac cggcgaccac agctgcttcg acgcagacgt
     4021 gctgctgcaa cccgaacgtt agcgggctta gctgggcgcg acatccaatc cgttggccgc
     4081 cactatcgcc gcgcgtgccc attcgtggcg ggacacagcc ggctggatgc ggtcgcggcg
     4141 aatcgtgtcg agatctatcg cccttccggc gatcaccgcg ttcggcaccc ggaaggagaa
     4201 ggccggctgg tcggtggcca cggccagcag ggcatcggaa tccgtgaaat tgttggcgcg
     4261 catagtgtcc tcggtccacc tcagcggctt gaggccgttg aagttcttga cgtacacgac
     4321 ccagagacgc gtgccctgcc cgttgtagag cttggtgagt gctcggttca cagcgtcgtg
     4381 ctcgagcggg ccgagcacac ccgattgatc ggtgatctgg ctggacagcg ggatggccgg
     4441 cgttgacggc ggcggcacgg cctgccctgg cggcggtgcg accaactgcg aaggggaact
     4501 cccgccgttg tgctggccgg cccgggcgtc gagcatgcga accccggcgt aggcgctcac
     4561 cgcgagcagg gcgaccaaga cgagcgcagc gacggcaatt gccggtcccc gccagctgcc
     4621 ccgcgctttt ggcgaggctg atggtgtgct cgccggtgcc gcgatcactt ccgtcggtgc
     4681 ggcgctcagc gtcatcggcg ccgcgctgat cgccgtcggt gcggccgccc ggtgggcggc
     4741 ggccgtgtcg atctggctac cgagtgccgc cgcgaaatcg gcgcatgtcg ggtaacggtc
     4801 ttcgggcttt ttggcgagtg ctttggcgat cgcgccgtcg agggaggcca gctcgggtcg
     4861 gcgcgtgctg atgtgcggcg gaggcgcgga caaatgctga gtgatgacca ccgccgggtt
     4921 ggaatggtca aacggggcgg acccggccag caggtcaaag gcggtgcagc cgagcgcgta
     4981 ttggtcggcc cgtccgtcca gatccgcacc ctgcagttgt tcgggcgcgc agtatgcggt
     5041 ggtgcccacc agcatgttcg tcgcggtcag accactgatg tcgcccagtt cccgggcgat
     5101 gccgaaatcg gcgagcagga tccgtcgtcg cgggttggct tcggtcagca ggatgttgga
     5161 cggtttgatg tcacgatgca gcaggccacg cgagtgcgcg taatcaagcg cgtcggccac
     5221 agccgagatg atctcgacga catcggcttt cggcatcccg gacggaaagc gggtccgcag
     5281 caatttcgcg gcgtccgtac cctccacgta gtccatcgag atccacaatt gcccctggta
     5341 ttcgccacgg tcgtggatgc cgacgatgtg cgggtggtac aggctcgcgg ccaggtcggc
     5401 ctccctgttg aaacgttggc gaaactcatc gttcgcggtc agctcgcccg gcaagacttt
     5461 gagggcgtcg cggcgcggta gccgggggtg ctgcgccaga tacacctggc ccatgccgcc
     5521 ggtccccaga cgccgaatga tggtgtatcc ggcgaagacc tgcccctctt tcaggccatc
     5581 ggtttcgggc atgtaggcat gctactgagg cggtgactgg gaaagacacg taattgccga
     5641 cagcggcgct tcgctcgggg cgcgcccgcg gctgacttcg atgagaatgg cgggcgatgc
     5701 taaagcgcgc gtcttggagc tcgtggaccg tggttgtacc ggtgctggcc gtcgtcacgc
     5761 tggtggcggc ctggggccgc catctcagcc cggtgctgat cgcggtggag gcgacgttcc
     5821 tcatcggtgc cgtgctggcc gcggtacacc acgcggaggt agtcgcgcac cgggtcggcg
     5881 agccgtacgg gtcgctggtg ctcgcagccg ccgtgacgat catcgaggtg gccctgatcc
     5941 tggcgctcat ggcgtccggg ggggacgagg ccgcgacgtt gcccagggac accgtgttcg
     6001 ccgcggtgat gatcaccatg aacggaatcg ccggcgtgtc gctgttgctt ggttcccggc
     6061 gttacggcgt gaccacgttc aacgccgagg gcagcggggc ggcgctggcc acgctcacca
     6121 cgctggcgac gctcagcctg gtgctgccca cgttcaccac tagccatggg ggcctggagt
     6181 tctcacccgg tcagttgatc ttcgccgcgg tcgcctcact ggggctctac ctgctgtttg
     6241 tgttcatgca aaccgtgcgg catcgcgact tctttctgcc ggtcgcgcag aagggccaga
     6301 aaggcttgtt cgaggacgaa acccacgcgg atccgccgag cacttcggcc gcgttgcgga
     6361 gccttggggt gctgctgctc gctttggtgg cggtggtggg tctggccgaa caagagtcgc
     6421 cggcaatcga gcatgtggtg tcagcggccg ggttcccgca gtcattcgtc ggcgtggtga
     6481 tcgcgagcct ggtgctgctg ccggagacgc tcgcggcggc gcgggcggcg cggcaaggcc
     6541 gcatccagac cagcctcaac ctggcctatg gctcttcgct ggccagcatc gggctcacca
     6601 ttccgaccat cgcgctgtcc gagatttggc taaaagggcc gttgttgctc ggccttgctc
     6661 ccacgcagat tgtgttgttt gcgctgaccg tcgtgatcag cgtgctgacc gtggtcccgg
     6721 gccgggccac ccggctgcag ggcgaggtgc acctggtatt gctcgccgct tacgtgttcc
     6781 tggcgatcgt tccctaacgt gttgcgcgag cataggtgac acaaattcac tttcgagccc
     6841 agtccggact gagctgtgcc gcctaaggga cggcaaacgc ttagttccga atcagtcatt
     6901 ttccttcccc tcgtcgatgc ggcatggaat cgtttgccga tgacggtgtg gccaatgcgt
     6961 gacagtgaac gtgaatagga gagtgtgccg tggcggcgaa gaaacccgag gacaagcccg
     7021 acatcccgat caatgcggaa ttggctcgga tggatgccga gggcgttgac gtcgccgacg
     7081 tgatcgcatt ggccggctac gttggtcgat cgccgaaaga aggggtggtc cggctgcacc
     7141 cgggccttca ggacttgtcc atcagcatcg acatcgccgc ctccgatatc gtggcgaccc
     7201 gagaagcacc cgctgcgacg atgcccttgg gcggcgtcgt cctgtgggtg tcaggcagcg
     7261 cggatgtgac cttccgtcga acacgcacag tcgttgcggc ggcacaggaa gtcagagggt
     7321 ttttcggcgc cggccccgcg ctggaaccgt ccagcaacga cccggtcgac cgcctcaaca
     7381 tccaggtgcg tc
//
LOCUS       DASSGQ010000121        10928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_737991,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000121 DASSGQ010000000
VERSION     DASSGQ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737991"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1326
                     /locus_tag="VFI55_05665"
     CDS             136..1326
                     /locus_tag="VFI55_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HET7073554.1"
                     /translation="MPEINRRRFLIASAGVGATGLLSGAVAVSWPDLMRAGQDRPLAA
                     GAGVLVIVSMYGGNDGINTLIPYADNAYHDARPELAYAPGDVLHLDQQLGLNPAMKGL
                     AQLWGQRQLAIVRGVSYPQPDHSHFRSMDIWQTASPAEPVSTGWIGRWLDAIGDDPLR
                     AVNIGAVLPPLAVGAEYTAAALTPSGAAAKAEKFDTIMTALGNDDPHDTPAMAAVCKA
                     YRAARTADTTFASVKSSTEGHNSLATQLSLVASAVKARVPTRVYTVQLSGFDTHANER
                     DTQQRLLQTFDDAVAGFLRDMAGDPYGKNVVVMAYSEFGRRVRANASQGTDHGTAGPV
                     FVAGAPVKGGFYGEEPSLTNLDNGDLKSTTDFRDVYYELLTRTVGTDPTPSVGAGRTA
                     LGFL"
     gene            complement(1340..2350)
                     /gene="fgd"
                     /locus_tag="VFI55_05670"
     CDS             complement(1340..2350)
                     /gene="fgd"
                     /locus_tag="VFI55_05670"
                     /EC_number="1.1.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase
                     (coenzyme-F420)"
                     /protein_id="HET7073555.1"
                     /translation="MAELKLGYKASAEQFAPRELVELAVAAEGHGMDSATVSDHFQPW
                     RHEGGHAPFSLAWMTAVGERTKRITLGTSVLTPTFRYNPAVIAQAFATMACLYPDRIF
                     LGVGTGEALNEIATGYQGDWPEFKERFARLRESVRLMRELWRGDRVDFDGEYYRLKGA
                     SIYDVPEGGVPIYIAAGGPAVAKYAGRAGDGFICTSGKGEELYKDKLIPALKEGAAAA
                     NRDVDDIDKMIEIKISYDPDPALALENTRFWAPLSLTAEQKHSIDDPIEMEKAANALP
                     IEQVAKRWIVASDPDEAVEKVGQYVTWGLNHLVFHAPGHDQRRFLELFEKDLAPRLRR
                     LA"
     gene            2471..3217
                     /locus_tag="VFI55_05675"
     CDS             2471..3217
                     /locus_tag="VFI55_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7073556.1"
                     /translation="MGPSPTLVQVTDTVHLARGHAVNWTLVAGDTGVMLIDAGYPGDR
                     EDVLASLRKLGYDAGDVRAILLTHAHIDHLGSAIWFAAEHGTPVYCHADEVGHAKREY
                     LEQVSVFDVAVRIWQPRWAVWGVHVVRSGGLIRDGIPTTRPLSAEVAAGLPGHPMGIA
                     TPGHTSGHCSYVVDGVLASGDALITGHPLLRHRGPQLLPEVFSHSQPDCLRSLDALAL
                     LDTDILLPGHGDVWRGPIREAAKAALQRAG"
     gene            complement(3241..4629)
                     /locus_tag="VFI55_05680"
     CDS             complement(3241..4629)
                     /locus_tag="VFI55_05680"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255635.1"
                     /note="This protein belongs to a clade of FMN-dependent
                     monooxygenases, within a broader family of
                     flavin-dependent oxidoreductases, the luciferase-like
                     monooxygenase (LMM) family, some of whose members use
                     coenzyme F420 rather than FMN; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NtaA/DmoA family FMN-dependent monooxygenase"
                     /protein_id="HET7073557.1"
                     /translation="MDVREGKHRKPIHLAAHFPGVNNTTVWTDPSAGSQIEFDSFVHL
                     ARIAERGLFDFFFLAEGLRLREHRGRIYDLDVVGRPDTFTVLAALAGVTDRIGLTGTI
                     NTTFNEPFEVARQFATLDQLSGGRAGWNMVTSSDAFTGANFRRGGFLDHADRYSRAEE
                     FLTVARQFWDSWEVDAAPADVDQGVYVDPGRIRSVRHRGPQFDVRGFATLPAGPQGHP
                     VLLQAGDSADGRAFGARHADALFTLHGSLEHGQRYYADVKGRAASYGRDPNQLKVFPA
                     ATFVIGDTDDQAQDKARLIRYQQVSGATAIAMLEQVWGRDLSGHDPDGPLPDFDPAVD
                     SDITQGRVRHGDPVAVARRYRERAEAENLSIRELIIAVTSRQQFVGTPKRIADEIDQY
                     IQADACDGFILVPHLTPHGLDEFVDRVVPLLQERGAFRAEYTGETLREHLGLEAQGHA
                     TFESASACDSTF"
     gene            complement(4629..5747)
                     /locus_tag="VFI55_05685"
     CDS             complement(4629..5747)
                     /locus_tag="VFI55_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7073558.1"
                     /translation="MTRVPLSVLDLAPISAGSDVATALRNTIDLAQNAERWGFHRFWI
                     AEHHFVGVASSSPAVLIGQIANATNTIHVGSAAVQLGYTTVPAVVEGFGMLEAFYPGR
                     IDLGVGRGQRRDSGFKPKQPSTKSRPPRQRREVDGVVIPLPFDLRGLLDKERVQATMG
                     ILQQPEAVVPDFSEQLADIIAMLDGTYQVDGFDVHAVPGERSGLTPWIFGSTRGQSAK
                     LAGARGLPFVASYHITPATALEAVDEYRDAFVPSAALKRPYVVVSADIVVADDTATAR
                     HLATGYGQWVYSIRTGVGAMPYPDPDDCPPLTDEQLPVVRDRLETQFVGNPDEVAERL
                     SALQRVTGADELVVTSVTHRHEDRLRSHELIAKRWGLN"
     gene            complement(5744..6841)
                     /locus_tag="VFI55_05690"
     CDS             complement(5744..6841)
                     /locus_tag="VFI55_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7073559.1"
                     /translation="MTDELHLGVALDGYGWHPQAWRATLASDPGTPSVLSGRYWAKLA
                     AIAEGGLLDFLTIDDTLMPQSGRRERIDPGRLAGRADAVLVAARIAPATQHIGLIPVA
                     TVTHTEPFHVSKSIATLDYVSRGRAGWQARVSVTAHEAALFGRRDGSQIALFEEAVEY
                     VDVVRRLWDSWEDDAIIRDVATGRYVDIDKLHYVDFTGKHFSVKGPSITPRPPQGQPV
                     VAALAHAGPVYEFAATSADVVFITPTDGAAVQRILGEVHASGGGALKVFADVVVSFGG
                     DADFKSDALVFSGSATELVDMLLDWQLLGIDGARLRPAVNAADLPVIVDEVVPLLQRA
                     GRFRTGYRSGETLRARLHLPVAPNRYAAVRR"
     gene            complement(6838..7344)
                     /locus_tag="VFI55_05695"
     CDS             complement(6838..7344)
                     /locus_tag="VFI55_05695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7073560.1"
                     /translation="MSGSTQAASGQLRFVLAAQDDPLAQPLLAELAVEYAQRYGGTPT
                     AHLGWLPVPTVELAPPDGGLLIGVLDDGPVTGGAFRRFDADTAELKRIWTDRAHRRRG
                     YARALLAALEAETAARGYRRLYLITGNRQPEAEALYDATGYTRVVPVEPLPSWGPFRP
                     IAFEKWLA"
     gene            complement(7423..7794)
                     /locus_tag="VFI55_05700"
     CDS             complement(7423..7794)
                     /locus_tag="VFI55_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HET7073561.1"
                     /translation="MQRLGRLAYLSADVVGVLVFCAVGRRSHDEGLSVTGVATTAWPF
                     LSGTVVGWLVSRAWRRPTAVVPTGVIVWLCTVAVGMGLRKASSAGVAASFVVVAASVT
                     AVLLLGWRAVVGLTRRRRSDV"
     gene            7868..9076
                     /locus_tag="VFI55_05705"
     CDS             7868..9076
                     /locus_tag="VFI55_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET7073562.1"
                     /translation="MSTQAPPAFDRNDPLGLDASLSGDELAVRDTVRKFCAEHVTPYV
                     AEWFEIGDLPVRELGKRFGQLGLLGMHLHGYGCGGASAVHYGLACLELEAADSGVRSM
                     VSVQGSLAMFAIWKFGSEEQKQQWLPGLAAGELLGCFGLTEPDVGSDPGAMKTRARRD
                     GSDWVLNGRKLWITNGSVADVAIVWAATDGGDVQGDSPGIRGFIVPTETTGFTANTIH
                     HKLSLRASITSELVLDDVRLPAAAMLPEAKGLAGPLSCLSEARYGIIWGSMGAARTAW
                     QAALDYAMQRTQFGRPIAGFQLTQAKLVDMAVELHKGQLLSLHLGRLKDTVGLRPDQV
                     SFGKLNNTREAIKICRTARTILGGNGISLEYPVIRHMVNLESVLTYEGTPEMHQLVLG
                     QAFTGSDAFR"
     gene            9124..10341
                     /locus_tag="VFI55_05710"
     CDS             9124..10341
                     /locus_tag="VFI55_05710"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7073563.1"
                     /translation="MPIRRLACVVLALGIVAGCGAPRSESGPGDHAKADAVMRVVRNA
                     MAQAHLRSVIVRVTVDGKEIVTQATGDSMTGVPASTDMHFRNGAVAISYVSTLLLRLV
                     DEKKLSLDDTLSKWLPDFPHAQQVTLGQLAQMTAGYPDYVIGNEQFSNEVYANPFQQW
                     TTQDILAQISARPLLYDPGTNWNYAHTDYVLLGLALEKATAQDMPTLLQNEVLGPLGL
                     KATANSDTPEIPSPVLHAFSSERRQALKIPAGTPFYEESTYWNPSWTITHGAIQTTNI
                     YDLEATAVGVGSGKLLSPDSYKKMVSTSLRGKTHAQPGCPTCFDQNEGYTYGLGIITS
                     GDWLMQNPLFSGCAAVEAYLPAQKVAIAVAVTYAPEAFDDQGNYSNQADILFRKIGAV
                     LVPNDAPPMPPGR"
     gene            10344..>10928
                     /locus_tag="VFI55_05715"
     CDS             10344..>10928
                     /locus_tag="VFI55_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073564.1"
                     /translation="MNVVAHRIGVLAAACGLSLCAVAATPDAAADTVTLPPMASSNAG
                     PIIGGGNSTGISQQLTGLGNPNVQEVDGSDAAQFITAAAGVADPQLAAPFGLLRRVLT
                     CQNNNAGFGARAYRRNDGLWGGAMLVAAKSATPNVDALAACAKTNWRRPTVGGETAMC
                     DSGWTTPTAFESRGGEAYYILLAGTADDFCSALNG"
BASE COUNT         1841 a         3767 c         3606 g         1714 t
ORIGIN      
        1 tcggcgtcgg tgactggtca gaccgcaccg tcgacgcgct gcagcccttg gtgcgccaac
       61 ccccgcagtt ggtcgcggcc gccgtcaaca cacccgaata cctcacttcc tgagcgagat
      121 tggccgagat tgcccatgcc cgagatcaat cgccgcagat tcctgatcgc cagcgcgggc
      181 gtcggcgcga ccgggctgct gtccggtgcg gtggcggtca gctggcccga cctgatgcgg
      241 gcggggcaag accgcccgct ggccgccggc gccggagtgc tcgtcatcgt gtcgatgtac
      301 ggcggcaacg acggaatcaa caccctgatt ccctacgccg acaacgctta tcacgacgcg
      361 aggccggaac tcgcttacgc gcccggcgat gtgctgcacc tcgaccagca actggggctc
      421 aaccccgcga tgaagggttt ggcccaactg tggggccagc ggcaactcgc gatcgtgcgc
      481 ggagtgagct acccgcaacc cgaccacagc catttccggt ccatggacat ctggcagacg
      541 gcgtcccccg ccgagccggt ctcgaccggc tggatcggcc gctggctcga cgccatcggc
      601 gacgatccgt tgcgggcggt caacatcggg gccgtgctgc ccccactggc cgtgggggca
      661 gagtacacgg cggcagcgct tactccgagc ggcgcggcgg ccaaggccga gaaattcgac
      721 acgatcatga ccgcgttggg caacgacgat ccccatgaca ctccggcgat ggcggcggtg
      781 tgcaaggcct atcgcgccgc gcggaccgcc gacacgacgt ttgcttcggt caaatcgtcc
      841 accgagggcc acaattcgct ggccacgcag ctcagcctgg tggccagtgc ggtgaaggcc
      901 cgcgtcccga cgcgggtgta cacggtgcaa ctcagcgggt tcgacacgca cgccaacgaa
      961 cgcgacaccc agcagcgcct gctgcaaacc ttcgacgacg cggtggccgg gttcctgcgg
     1021 gacatggccg gcgaccccta tggcaaaaac gttgtggtga tggcgtattc ggaattcgga
     1081 cgccgggtgc gggccaacgc ttcgcagggc accgatcacg gcaccgccgg tcccgtcttc
     1141 gtcgccggtg cgcctgttaa aggcggcttc tacggcgagg aacccagcct caccaacctg
     1201 gacaacggag acctgaagtc caccaccgac tttcgcgacg tctattacga gctgctgacg
     1261 cgaacggtag ggaccgatcc cacgccttcg gtcggagccg gccgcacggc cctcggcttc
     1321 ctctgaaccc gcccgcccgt caggccagcc gtcgcagcct gggcgccagg tccttctcga
     1381 acagctccag gaaccggcgc tggtcatgcc cgggcgcgtg gaacaccagg tggttgagac
     1441 cccatgtcac gtactgaccg accttttcga ccgcctcgtc ggggtcggac gccacgatcc
     1501 agcgcttggc gacctgctcg atcggcagcg cgttggcggc cttctccatt tcgattggat
     1561 cgtcgatgct gtgcttctgc tcggcggtca gcgacagcgg cgcccagaac cgggtgttct
     1621 ccagcgccag cgcgggatcc gggtcgtagg agatcttgat ctcgatcatc ttgtcgatgt
     1681 cgtcgacgtc gcggttggcg gccgccgcgc cctccttcag cgccggaatc agcttgtcct
     1741 tgtagagttc ctcgcccttg ccggaggtgc agatgaagcc gtcaccggcc cgtccggcgt
     1801 attttgcgac ggccgggccg ccggcggcga tgtagatggg aacaccgccc tcgggcacgt
     1861 cgtagatcga ggcgcccttg agccggtagt actcgccgtc gaagtcgacg cggtcgccgc
     1921 gccacagctc acgcatcagc cgcaccgatt cgcgcagccg cgcgaaccgc tccttgaact
     1981 ccggccagtc accctggtat ccggtggcga tctcgttcag cgcctcgccg gtgccgacac
     2041 ccaggaaaat gcggtccgga tacaggcagg ccatggtggc gaaggcctgc gcgatgacgg
     2101 ccgggttgta ccgaaaggtc ggggtgagca ccgaggtgcc cagcgtgatc cgcttggtgc
     2161 gctcgccgac ggcggtcatc caggccagcg agaacggggc gtggccgccc tcgtggcgcc
     2221 acggctggaa gtggtcgctg acggtcgcgc tgtccatgcc gtgaccttcg gcggcgacgg
     2281 ccagttcgac cagctcacgg ggtgcgaatt gttcggcgga cgctttgtat cccagtttca
     2341 gttcagccac agctttcttt gtactcctct cctgagggtc gcgacccgcc gcgcccggct
     2401 tcgccgcgct tgcgatcgct cctgtcccaa cggtcgcgac ccgccgcgcc cggcttctac
     2461 actcgcgggc atgggaccgt cgccgactct cgttcaggtc accgacaccg tgcacctcgc
     2521 caggggccac gcagtcaact ggacgctggt cgccggcgac accggcgtca tgttgatcga
     2581 cgccggctac cccggcgacc gcgaagacgt actggcctcg ctgcggaaac tgggctacga
     2641 cgcgggcgac gtgcgtgcca tcctgttgac ccacgcgcac atcgaccatc tgggctcggc
     2701 gatctggttc gccgccgagc acggcacccc ggtgtactgc cacgccgacg aagtgggcca
     2761 cgccaagcgc gaatacctgg aacaggtctc ggttttcgat gtcgcggtgc gcatctggca
     2821 gccacgctgg gcggtgtggg gcgtccatgt ggtgcgcagc ggcggcctga tccgcgacgg
     2881 catcccgacc acccggccgc tcagcgccga ggtcgccgcc gggctaccgg gccacccgat
     2941 gggcatcgcc accccggggc acaccagcgg ccactgctcg tatgtcgtcg acggcgtgct
     3001 ggccagcggc gacgcgctga tcacgggcca cccgctgctg cggcaccgcg ggccccagct
     3061 gctgccggag gtcttcagcc acagccagcc cgactgccta cgcagcctgg acgcgctggc
     3121 cctgctggac accgacatcc tgctgccggg tcacggcgac gtgtggcgcg ggcccatccg
     3181 cgaggcggcg aaggcggcct tgcagcgcgc gggttgagct gcctcggccg cgcagctgtc
     3241 tcagaaagtg ctatcgcacg cgctagctga ttcgaaagtc gcatgcccct gcgcctcgag
     3301 cccgagatgc tcccgcagcg tttccccggt gtactcggcg cggaacgcgc cgcgttcctg
     3361 caggagcggc accacccggt cgacgaactc gtccagaccg tgcggggtca ggtgcggtac
     3421 caggatgaag ccgtcgcagg cgtcggcctg gatgtactgg tcgatctcgt cggcgatccg
     3481 ctttggcgtg ccgacgaact gctgccggct ggtcaccgcg atgatcagct cgcggatcga
     3541 caggttctcc gcctcggcgc gttcccggta gcggcgggcc accgcgacgg ggtcgccgtg
     3601 ccggacccgg ccctgggtga tgtcgctgtc gacggccgga tcgaaatcgg gcagcgggcc
     3661 gtccgggtcg tggcccgaca ggtcgcggcc ccagacctgc tcgagcattg cgatcgccgt
     3721 cgcgccgctg acctgttgat agcggatgag tcgcgctttg tcctgcgcct ggtcatcggt
     3781 atcgccgatg acgaaggtcg ccgccggaaa aaccttgagc tggttggggt ctcgaccata
     3841 ggacgccgcg cggcccttga cgtcggcgta gtagcgctga ccgtgctcga gcgacccatg
     3901 cagggtgaac agggcatcgg cgtggcgggc gccgaacgcg cggccgtcgg cggagtcgcc
     3961 ggcctgcagc aacaccgggt ggccctgcgg ccccgcgggc agcgtcgcga acccgcgcac
     4021 gtcgaactgg ggacctcggt ggcggacgct gcggattcgt ccgggatcca catagacccc
     4081 ctgatcgacg tcggccggcg ccgcgtcgac ttcccagctg tcccagaatt ggcgcgccac
     4141 ggtcagaaac tcctcggccc gcgagtaccg gtcggcatgg tcgagaaagc cgccgcgccg
     4201 gaaattggcg ccggtgaagg catctgacga ggtcaccatg ttccagccgg cgcggccacc
     4261 ggacaactgg tccagggtcg cgaattgcct tgccacctcg aacggttcgt tgaaggtggt
     4321 gttgatggtt ccggtcagcc cgatccggtc ggtgacaccg gccagcgcgg ccagcacggt
     4381 gaaggtatcc gggcggccca ccacgtcgag gtcgtagatc cgaccgcggt gctcgcgcag
     4441 tcgcagcccc tcggccagga aaaagaagtc gaacaatccg cgttcggcaa tccgcgcgag
     4501 gtgcacgaac gagtcgaact cgatctggct gcccgcgctg gggtccgtcc acaccgtggt
     4561 gttgttcacc cccgggaagt gggctgccag atgaataggc ttgcggtgct tgccttctcg
     4621 gacatccatc agttcagccc ccagcgtttg gcgatcagct cgtgcgagcg cagccggtct
     4681 tcgtggcggt gtgtcaccga ggtgacgacg agttcgtcgg cgccggtcac acgttgcagc
     4741 gcagacagcc gctctgcaac ctcatcggga ttaccgacga attgcgtttc cagcctgtcc
     4801 cttaccaccg gcagctgttc atccgtcagc ggcgggcaat cgtcgggatc cggatagggc
     4861 atggcgccga cgccggtccg gatggaatac acccattgcc cgtagccggt ggccagatgc
     4921 ctggccgtcg cggtgtcgtc ggcgaccacg atgtcggcgg acaccacgac gtagggccgc
     4981 ttcagcgcgg ccgaggggac gaaggcgtca cggtattcgt cgacggcctc cagggccgtc
     5041 gccggagtga tgtgatagct ggccacgaac ggcaagcccc gcgcgccggc cagcttggca
     5101 ctctgacccc tggtgctgcc aaatatccac ggtgtcaacc cggatcgctc gcccggcacc
     5161 gcgtggacat cgaaaccgtc gacctgatag gtaccgtcca gcatcgcgat gatgtctgcg
     5221 agctgctcgc tgaagtcggg caccactgcc tcgggctgtt gcaggatccc catggtggct
     5281 tgcacccgtt ccttgtcgag aagcccacgc aggtcgaagg gcagcgggat cacgacgccg
     5341 tcgacttcgc gccgctgtcg cggcgggcgg cttttggtcg acggctgctt cggcttgaac
     5401 ccgctgtcgc gccgttgccc tcggccgacg cccaagtcga tgcggcccgg atagaaagcc
     5461 tcgagcatcc cgaaaccctc gaccaccgcc ggtacggtcg tgtagcccag ctgcacggcg
     5521 gccgagccaa cgtgaatcgt gttggtagcg ttggcgattt gcccgatcag cactgccggc
     5581 gacgagctgg cgacaccgac gaagtgatgt tcggcgatcc agaaccgatg aaaaccccac
     5641 cgctcggcgt tttgggccag atcaatggtg ttccgcaatg ccgtcgcgac atcgctgccg
     5701 gcgctgatcg gggccaggtc caggaccgag agcggaacgc gggtcatcgg cgcaccgccg
     5761 cgtagcggtt gggcgccacc ggcaggtgca gccgtgcgcg tagcgtttcg ccgctgcggt
     5821 agccggtgcg gaagcgccca gcccgctgca ggagcggcac cacctcgtcg acgattaccg
     5881 ggagatcggc ggcgttgacg gccggccgca gccgcgcacc gtcgatgccc agcaactgcc
     5941 aatccaacag catgtccacc aattccgttg cgctgcctga gaagacgagc gcgtcagact
     6001 tgaagtcagc gtcaccgccg aacgacacca ccacgtctgc aaagactttc agcgcgccgc
     6061 cacccgacgc gtgcacctcg ccaaggatgc gctgcaccgc ggcgccgtcg gtgggcgtga
     6121 tgaagacgac gtcggcgctg gtggcggcga actcatagac gggaccggcg tgcgccaggg
     6181 ctgccactac cggctggccc tgcggtgggc gcggcgtgat cgacggccct ttgacggaga
     6241 agtgttttcc ggtgaagtcg acgtagtgca gcttgtcgat gtcgacgtag cgtccggtgg
     6301 ccacgtcgcg gatgatcgcg tcgtcctccc agctgtccca caaccgacgc accacgtcga
     6361 cgtactcgac ggcctcctca aacaacgcga tttgagagcc gtcccggcgg ccgaacaggg
     6421 ccgcttcgtg ggctgtgacg ctgacgcgcg cctgccagcc ggctcggccg cgcgacacat
     6481 agtcaagggt ggcaatggat ttggagacat gaaaaggctc ggtgtgggtg acggtggcca
     6541 ccgggatcag cccgatgtgc tgcgtcgcgg gggcgatgcg ggcggcgacc agcaccgcgt
     6601 cggcgcgacc ggccagccgg cccggatcga tgcgctcacg ccgcccggac tgcggcatca
     6661 acgtgtcgtc gatggtgagg aagtcgagca gcccaccctc ggcgatcgcg gccaacttgg
     6721 cccagtagcg accgctgagc accgacgggg tgccggggtc tgaggccagc gtggcccgcc
     6781 acgcctgcgg gtgccagcca tacccgtcga gggcgacgcc caggtgcaat tcatcggtca
     6841 tgccagccac ttttcgaatg caatcgggcg aaacggcccc caggacggca gcggctcgac
     6901 gggcaccacg cgcgtgtagc cggtcgcgtc atacagcgcc tcggcctcag gttggcgatt
     6961 gccggtgatc aggtacagcc gccggtaccc gcgcgcagcg gtctcggctt ccagcgccgc
     7021 cagcagcgcc ctggcgtaac cgcggcgacg atgcgcgcgg tcggtccaga tccgcttcag
     7081 ttcggcggtg tcggcgtcga aacgccgaaa cgcgccgccg gtgaccggcc catcgtccag
     7141 cacgccgatc agcaagccgc cgtccggcgg cgccaactcg accgtcggca cgggcagcca
     7201 gcccaaatgc gcggtcggcg tgccgccgta ccgctgcgca tactcgaccg ccagctccgc
     7261 gagtagcggc tgtgccagcg gatcgtcctg agcagccaac acgaaccgca gctgaccgga
     7321 agcggcctga gtggatccgg acatcacctc acgtctaacg ctcgtcgtcg agaagcattc
     7381 ctggctggct tccctggcga ccgcgccgaa tgtcatcgct gatcagacgt cggagcggcg
     7441 ccgccgggtc agcccgacga ccgcccgcca accgagaagc agaaccgcgg tgaccgacgc
     7501 ggccaccacc acgaaactcg ccgccacccc cgctgagctg gccttgcgca accccatgcc
     7561 gaccgccacc gtgcacagcc agacgatcac cccggtgggg accacggcgg tgggtcgccg
     7621 ccaggcgcgg gataccagcc acccgacgac ggttccgctc agaaacggcc atgccgtggt
     7681 cgcgacgccg gtgacgctga gtccttcgtc gtggctgcgg cggccgacgg cacaaaacac
     7741 caaaacacca acgacatctg cggaaaggta cgccagcctc cccagccgct gcatgcagcg
     7801 agaataccgg tcggggccgg ggcggctcgc gcttggccag cggccggcta gtggcaggct
     7861 gagtcccatg agcactcaag cacctcccgc gttcgaccgg aacgacccgc tgggcctcga
     7921 cgcatccctg tccggcgacg agctcgcggt gcgcgacacc gtcagaaagt tctgcgccga
     7981 acacgtgacg ccctacgtcg cagagtggtt cgagatcggc gaccttccgg tgcgcgaact
     8041 cggaaagcgg ttcggccagc tcggcctact cggcatgcac ctgcatggct acgggtgcgg
     8101 cggggcctcc gccgtgcact acggcctggc ctgcctggag ctggaggccg ccgactccgg
     8161 ggtccggtcc atggtgtcgg tgcagggatc gctggcaatg ttcgcgatct ggaaattcgg
     8221 ctccgaagaa caaaagcagc agtggctgcc cggcctggcc gccggcgagc tgctcggctg
     8281 cttcgggctg accgaacccg acgtcggatc cgatcccggc gcgatgaaaa cccgggcgcg
     8341 gcgcgacggt tctgattggg tgctcaacgg ccgcaagttg tggatcacca atggctccgt
     8401 cgccgacgtg gcgatcgtgt gggccgccac cgacggtggc gatgtgcaag gcgacagccc
     8461 agggattcgg ggcttcatcg tgcccaccga gaccacgggc ttcaccgcca acaccatcca
     8521 ccacaaactg tcgctgcggg catcgatcac cagcgagctg gtgctcgacg acgtacgact
     8581 gcccgcagcc gcgatgctgc ccgaggcgaa gggtctcgcg gggccgttgt cctgcctgtc
     8641 cgaagcgcgc tacggaatca tctggggatc gatgggagcg gctcggacgg cctggcaagc
     8701 cgcgctcgac tacgccatgc agcgcaccca gttcggccga ccgatcgccg gattccagct
     8761 gacgcaggcc aaactcgtcg acatggccgt cgagctgcac aagggtcagc tgctttcgct
     8821 gcacctcggg cgcctcaaag acaccgtcgg gctgcgtccc gatcaggtca gtttcggcaa
     8881 gctcaacaac acccgtgagg cgatcaagat ctgccgcacc gcgcgaacga tattgggcgg
     8941 caatgggata tcgctggaat acccggtaat ccggcacatg gtcaacctgg aatcggtgct
     9001 gacgtacgag ggcacaccag agatgcacca gctcgtgcta ggtcaggcgt tcacgggcag
     9061 cgacgccttc cgctgaccgg aatacacggg agccgggcgc ggcgcctgct aggttgacag
     9121 atcatgccga tccggcgcct cgcgtgtgtg gttctcgccc tcgggatcgt cgccggttgc
     9181 ggcgcgccgc gctctgagtc gggccccggt gaccatgcca aggccgacgc ggtgatgcgg
     9241 gtcgttcgca acgccatggc gcaggcgcac ctgaggtcgg tcatcgtccg agtcaccgtg
     9301 gacggcaagg agatcgtcac gcaagccacc ggcgactcga tgacgggggt gccggccagc
     9361 accgacatgc acttccgcaa cggcgcggtg gcgatctcct atgtgtcgac gttgctgctg
     9421 cggctcgtcg acgaaaagaa gctcagcctc gacgacaccc tgtccaagtg gctgcccgac
     9481 tttccgcacg cgcaacaggt caccctcggg caattggccc aaatgacggc cggctacccc
     9541 gactacgtga tcggcaacga gcagttcagc aacgaagttt acgcaaaccc gttccagcag
     9601 tggacaaccc aggacatcct cgcccagatc tcggcacggc cactgctcta cgatccggga
     9661 accaactgga actacgcgca cacggattac gtcctgctgg gcttggcgtt ggagaaggcc
     9721 accgcccagg acatgcctac gctgctccag aacgaggtgc tgggaccgtt gggcctgaag
     9781 gccaccgcca actccgatac acccgaaatc ccctcccccg tgctgcacgc attcagctcg
     9841 gagcgccgtc aagcactgaa aatccctgcc ggaacaccgt tctacgaaga atcgacgtac
     9901 tggaacccgt cgtggaccat cacccacggg gccattcaga cgaccaacat ctacgacttg
     9961 gaggccaccg cggtcggtgt aggctcgggc aagctgcttt cgccggactc atacaagaag
    10021 atggtgtcga cttcgctgcg cggtaaaacg cacgcccagc cgggctgccc gacctgcttc
    10081 gaccaaaacg agggctacac ctatggactt gggatcatca cctccggcga ttggctgatg
    10141 cagaaccccc tgttctccgg ttgcgcggcc gtggaggcat acctgccggc gcagaaggtt
    10201 gcgatcgcag tcgcggtcac ctacgcccca gaggccttcg atgaccaagg caactacagc
    10261 aatcaggccg acatactgtt ccggaagatc ggtgccgtgc tggtaccgaa cgacgcccca
    10321 cccatgccgc cagggagata gccatgaatg tcgtcgcgca tcgaatcggt gtcctggccg
    10381 ccgcgtgcgg cctgtccctg tgcgccgtgg cagcgacgcc ggacgcagcc gccgacacgg
    10441 tcacgctgcc cccgatggca tcgagcaacg cgggcccgat catcggtggc ggtaacagca
    10501 ccgggatctc gcagcaactg accgggctgg gcaacccgaa tgtccaggaa gtcgatggct
    10561 cggatgcggc ccaattcatc actgccgcag ccggagtcgc cgatccgcag ctcgccgccc
    10621 cgttcgggct gctgcgtcgg gtgttgacat gccagaacaa caacgccgga ttcggggccc
    10681 gcgcctaccg acgcaacgac gggctatggg gaggcgcgat gctggtcgcc gccaagagcg
    10741 ccacgccgaa cgtcgacgcg ctggcagcct gcgccaaaac gaattggcgt aggcccacgg
    10801 tcggcgggga gacggcgatg tgcgacagcg gctggaccac ccccacggcg ttcgaaagcc
    10861 gcggcggtga agcctattac atcttgcttg ccggcacggc cgatgacttc tgcagcgccc
    10921 tcaacggt
//
LOCUS       DASSGQ010000122        12489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1193822,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000122 DASSGQ010000000
VERSION     DASSGQ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12489
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193822"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /gene="pgk"
                     /locus_tag="VFI55_05720"
     CDS             <1..893
                     /gene="pgk"
                     /locus_tag="VFI55_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET7073565.1"
                     /translation="EVALLENVRFEPAETSKIDEERAVLAAQLAALGDLYVGDGFGVV
                     HRKQASVYDIATLLPHYAGTLVAREIQVLEQLTSSTKRPYAVVLGGSKVSDKLGVIES
                     LATKADSIVIGGGMCFTFLAAEGYSVGKSLLEEEMVDTCRRLLDTYADVLRLPMDIVV
                     AEKVAADAAPQIVPADAIPGDLMGLDIGPGSIKRFAALLSNARTIFWNGPMGVFEVPA
                     FAAGTKGVAEAIAAATAKGAFSVIGGGDSAAAVRALGIPESDFSHISTGGGASLEYLE
                     GRTLPGIEVLGRPQPTEGDS"
     gene            890..1675
                     /gene="tpiA"
                     /locus_tag="VFI55_05725"
     CDS             890..1675
                     /gene="tpiA"
                     /locus_tag="VFI55_05725"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET7073566.1"
                     /translation="MSRKPLIAGNWKMNLNHFEAIALVQKIAFALPDKYYDKVDVTVL
                     PPFTDLRSVQTLVDGDKLRLTYGAQDLSQHDSGAYTGDISGAFLAKLGCSFVVVGHSE
                     RRTYHDEDDALVAAKAAAALKNGLTPIVCLGEHLDVREAGDHVSHCELQLRGSLAGLS
                     AEQIGTVVVAYEPVWAIGTGRVASGADAQEVCAALRKELASLASPEIADTVRVLYGGS
                     VNAKNIGDIIARADIDGALVGGASLDGEQFATLAAIAAGGPLP"
     gene            1748..2314
                     /locus_tag="VFI55_05730"
     CDS             1748..2314
                     /locus_tag="VFI55_05730"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7073567.1"
                     /translation="MSSIRIFILAALEERGPMHGHQLRLLAEEEHVGLWTDITVGGLY
                     GAIKRLAAEDLIEEIRVERAGAYPQRQVWAVTEAGREAVGALRLRALREIVVKPDPFD
                     LAITGVHPDHLDDLPTMIAARIGSLNAMLADWEAHAATIDRYLTEAEKLVMRHRADRL
                     RAEIAWHEELSEALPGIALQQRARAEQT"
     gene            2311..3753
                     /locus_tag="VFI55_05735"
     CDS             2311..3753
                     /locus_tag="VFI55_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020111608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7073568.1"
                     /translation="MTAPTPKTVVTERRNWLALAVLLVGAFMALLDTTIVNVAIPTIR
                     ASLAASNATLSWIISGYALAFGLALIPAGRVGDRLGHKWVFIAGLSLFTVASLACGLA
                     TDDMQLVSARAVQGLAGGMFFTPITALIQLMFTGRERARAFAVMGATIGFSVALGPLV
                     GGLIIAASGADAGWRLVFDVNVPLGVLAVVAAVLLLPAGAENPDTAADWPGLVLLSAA
                     LVALLTPLIQGQQEGWPFWTYASMAGAVALLAVFALWERHVETTGGNPLVPPRLFVHA
                     SFSGGVLLALVYFAAFTSIFFTIALLWQAGLGHTALQSGLVVTPFAVGLIGGASRSDA
                     LAERFGRAVIVAGLGMVAVGITAVWFLLARMPSTGYSGWQLVAPLLIAGVGSGLFIAP
                     NVDFIVATVDPQDAGAASGVIGTMQRVGSAIGIAIIGTILFGTLHVNRAGPDAVARAF
                     GHSATLAMAASAGLSIAAFALGFALPKRTG"
     gene            3806..4039
                     /gene="secG"
                     /locus_tag="VFI55_05740"
     CDS             3806..4039
                     /gene="secG"
                     /locus_tag="VFI55_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET7073569.1"
                     /translation="MQLALQITLVVTSILVVLLVLLHRAKGGGLSTLFGGGVQSSLSG
                     STVVEKNLDRLTVFITGIWLVCIIGMGLLIKYR"
     gene            4124..6931
                     /gene="ppc"
                     /locus_tag="VFI55_05745"
     CDS             4124..6931
                     /gene="ppc"
                     /locus_tag="VFI55_05745"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET7073570.1"
                     /translation="MVEVSDTTLEPIGAVQRTRIGREATEPMRADIRLLGTILGNTVR
                     EQSGDEVFDLVERARVESFRVRRSEIDRAEMARMFDGIDIHLAIPVIRAFSHFSLLAN
                     AAEDIHRERRRAIHVDAGEPPQDSSLAATYAKLDLAELDSPTVAGALRGALVSPVITA
                     HPTETRRRTVFVTQHRITELMRLHAAGHTETDDGRSIELELRRQVLTLWQTALIRMSR
                     LQITDEIEVGLRYYPAALFRVIPQVNAEVRDALRARWPDADLLSAPILQPGSWIGGDR
                     DGNPNVTADVVRRATGSAAFTALAHYLAELTDLEQELSMSARLITVTGQLTALEQSCP
                     EKARADEPYRRAVRVIRGRLTATAADILDWQPQHELDLGLPPYSAPAELRADLDVVDA
                     SLRMHGAALLADDRLARLREGVHVFGFHLSGLDMRQNSDVHEEVIGELLAWAGVHPDY
                     GSLPEDERVELLTGELGTRRPLVGDRAQLSDLARSELGVVAAAAQAVERYGPAAVPNY
                     IISMCQSLSDILEAAILLKEAGLLDASGPEPYCPVGISPLFETIEDLHNGASILQAML
                     DLPLYRSLVDARGGCQEVMLGYSDSNKDGGYMAANWAVYRAELTLAEVARKTGIRLRL
                     FHGRGGTVGRGGGPSYQAILAQPPGAVNGSLRLTEQGEVIAAKYAEPELARRNLESLV
                     AATLESTLLDVEGLGDAAEPAYAVLDEIAILAHRAYAELVHETPGFVEYFKASTPVSE
                     IGSLNIGSRPTSRKPTASIADLRAIPWVLAWSQSRVMLPGWYGTGSAFEQWIAAGPET
                     AAERVAILHELYERWPFLRSVLSNMAQVLAKSDLGLAARYAELVPDEALRHRVFDKIV
                     DEHRRTIAMHKLITGHEDLLADNPTLARSVFNRFPYLEPLNHLQVELLRRYRSGDDDE
                     LVQRGILLTMNGLASALRNSG"
     gene            complement(6957..7355)
                     /locus_tag="VFI55_05750"
     CDS             complement(6957..7355)
                     /locus_tag="VFI55_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HET7073571.1"
                     /translation="MTVMADRSGRSGSERNRIRTLTQAALNADKTVEQVEDVLDGLGK
                     TMKELSSSLSALNATVERLEGGLDHLEGTLQNLDELARRLIALVEPVEAIVGRIDDMV
                     KVGETVMSPLSVTEHAVRTVLDRLRNRTVE"
     gene            complement(7398..8141)
                     /gene="pgl"
                     /locus_tag="VFI55_05755"
     CDS             complement(7398..8141)
                     /gene="pgl"
                     /locus_tag="VFI55_05755"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET7073572.1"
                     /translation="MSKVVEVFADSEALVDAAGGRLVETIKTAVAARGRALIVLTGGG
                     NGIGLLQYLGRHRQLIDWSKVHLFWGDERYVPEDDDERNDKQARAALLDHINIPSSHV
                     HTMPASDGEFGSDLAAAALAYEQLLAANADDGQRVPNFDVHLLGMGPEGHINSLFPDT
                     PAVRETTHMVVAVEDSPKPPPQRITLTLPAIQRSREVWLLVSGAGKAEAAAEAIGGAS
                     PVSVPAAGAIGLQTTLWLLDEQAASKLPG"
     gene            complement(8138..9049)
                     /gene="opcA"
                     /locus_tag="VFI55_05760"
     CDS             complement(8138..9049)
                     /gene="opcA"
                     /locus_tag="VFI55_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HET7073573.1"
                     /translation="MIVDLPGTTTTAVNKKLDELREKIGAVTMGRVLTLIIAPDSEEV
                     LEQSLKAANDASHEHPSRIIVTMTGDPHAEDARLDAQLRAGGDAGAGEIVVLRLSGPL
                     AGHASSVVIPFLLPDIPVVAWWPDVAPAVPAQDPLGKLAIRRITDATNGPDPLLAIKG
                     RLPGYTAGDTDLCWARITYWRALLTSAVDQPPHEPIESALVSGLKNEPALDILAGWLA
                     SRIDGPVRRAVGELKVELARKSETIALTRPQEGRTATLSRTAKPEALVPLARRETGEC
                     LAEDLRRLNADDIYLTALEGIKKVQYV"
     gene            complement(9105..10670)
                     /gene="zwf"
                     /locus_tag="VFI55_05765"
     CDS             complement(9105..10670)
                     /gene="zwf"
                     /locus_tag="VFI55_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET7073574.1"
                     /translation="MSPANSKAGTRGAVAEWQNPLRDKKDKRLPRIAGPCALVLFGVT
                     GDLAHKKVVPAIYDLANRGLLPPTFSLVGFARRDWDHDSFAQVIHDDVKGHCRTPFRQ
                     AVWERLADGLRFVRGEFDDDAAFARLGETLDKLDAERGIGGNHVFYLAIPPKSFPVVC
                     EQLHKSGLARPQEDRWSRVVIEKPFGHDLQSACDLNQVVNSVFPEESVFRIDHYLGKE
                     TVRNILALRFANQLFDPVWNAHYVDHVQITMAEDIGLGGRAGYYDGIGAARDVIQNHL
                     MQLLALTAMEEPVSFHPKALQTEKIKVLSATQLALPLDETSSRGQYAAGWQGGEKVVG
                     LLDEEGFAKDSTTETFAAITLEVDTRRWAGVPFYLRTGKRLGRRVTEIALVFKRAPHL
                     PFDATMTDELGANALVIRVQPDEGITLRFGSKVPGTAMEVRDVNMDFSYGSAFAEESP
                     EAYEALILDVLLGEPSLFPVNAEVELAWEILDPVLENWAAHGKPDPYESGTWGPESSF
                     EMLRRTGREWRRP"
     gene            complement(10667..11788)
                     /gene="tal"
                     /locus_tag="VFI55_05770"
     CDS             complement(10667..11788)
                     /gene="tal"
                     /locus_tag="VFI55_05770"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HET7073575.1"
                     /translation="MTSQNPNLAALSAAGVSVWLDDLSRERLQSGNLQELIDTKSVVG
                     VTTNPSIFQKAFAEGDAYEGQIAELAERGADVDATVRTVTTDDVRTACDVLAPQWEAS
                     DGVDGRVSIEVDPRLAEETDKTIAQAVELWKIVDRPNLFIKIPATKAGLPAITAVLAE
                     GISVNVTLIFSVERHRLVMDAYLAGLEKARQAGHDLSKIHSVASFFVSRVDTEIDKRL
                     EKIGSEEALGLRGKAGVANARLAYAAYQDVFDDGDRYGALKSDGARVQRPLWASTGVK
                     NPAYSDTLYVTELVAAHTVNTMPEKTIDAVADHGVVTGDTISGTAAAAQEVFDKLTAV
                     GIDLTDVFVVLEKEGVDKFVESWTELLGETQKQLGSATK"
     gene            complement(11804..>12489)
                     /locus_tag="VFI55_05775"
     CDS             complement(11804..>12489)
                     /locus_tag="VFI55_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET7073576.1"
                     /translation="YMRPSVRLASLMNIDTIYVWTHDSIGLGEDGPTHQPIEHLSALR
                     AIPHLSVVRPADANETAYAWRTILARGNGSGPVGLILTRQGLPVLEGTNIEGVARGGY
                     VLGDDGGDEPDVVLIATGSEVQLAVEAQNLLADRDIVARVVSMPCVEWFESQPSDYCD
                     SVLPPSVSARVAVEAGIAQSWHKLVGDTGKIVSIEHYGESADAKTLFREFGFTAEAVA
                     AAAEQVVDN"
BASE COUNT         1972 a         4119 c         4194 g         2204 t
ORIGIN      
        1 gcgaggtcgc gctgctcgag aacgtccgct tcgagcccgc ggaaacctca aaaatcgatg
       61 aggaacgggc ggtgctcgcc gcccagctgg ccgcgctcgg cgacctctac gtgggtgacg
      121 ggttcggggt ggtgcaccgc aagcaggcct ccgtgtacga catcgccacc ctgctgccgc
      181 attacgccgg cacgctggtg gctcgggaaa tacaggtgtt ggaacagctg acgagctcaa
      241 ccaaacgccc ctacgcggtg gtgctgggcg ggtcgaaggt gtccgacaaa ctcggcgtca
      301 tcgagtcgct ggccaccaaa gccgacagca tcgtgatcgg cggcggcatg tgtttcactt
      361 tccttgccgc agaaggctat tcggtgggca agtcgctgct ggaagaagag atggtggaca
      421 cctgccgcag gctgctggac acctacgccg acgtgctgcg actgccgatg gacatcgtgg
      481 tggccgagaa ggttgccgcc gacgcggctc cacagatcgt cccggccgac gccattccgg
      541 gcgatctgat gggattggac atcggacccg gatcgatcaa gcggttcgcg gcattgctgt
      601 ccaacgccag gaccatcttc tggaacggcc cgatgggggt gttcgaggtg ccggcgttcg
      661 ccgccggtac caaaggtgtc gccgaggcga tcgccgcggc caccgccaag ggcgcattca
      721 gcgtgatcgg cggcggggac tccgcggccg cggtgcgcgc gctcggcatc ccggagagcg
      781 acttctcgca catatccacc ggcggtgggg cgtcgttgga atacctggaa ggcaggacgc
      841 taccggggat cgaggtactg ggccggcccc agccaaccga aggagactcg tgagccgcaa
      901 gccgctgatc gccggcaact ggaagatgaa cctcaatcac ttcgaggcga tcgcgctggt
      961 gcaaaagatc gcattcgcgt tgccggacaa gtactacgac aaagttgacg tcacagtcct
     1021 gccgccgttc accgacctgc gcagcgtgca gaccctggtc gacggcgaca agctgcggtt
     1081 gacctatggc gctcaggacc tctcgcagca cgactcgggc gcctacaccg gcgacatcag
     1141 cggcgccttc ctggccaagc tcggctgcag cttcgtcgtc gtcgggcact ccgagcggcg
     1201 tacctaccac gacgaggacg acgcgctggt ggccgccaag gccgcggccg cgctcaagaa
     1261 cggattgacc ccgatcgtgt gcctcggcga gcatctcgac gtccgggagg cgggcgacca
     1321 cgtcagccac tgcgagctgc agctgcgcgg ctcgctggcg gggctgtccg ccgagcagat
     1381 cggcaccgtg gtcgtcgcct atgagccggt gtgggcgatc ggcaccgggc gggtggccag
     1441 cggcgccgac gcccaggagg tatgtgcggc gctccgcaaa gagttggcgt ccctcgcctc
     1501 cccggagatc gccgacaccg tgcgggtgct gtacgggggc tcggtgaacg ccaaaaacat
     1561 cggcgacatc atcgcgcgcg cggatatcga cggggctttg gtcggcgggg cgtcgttgga
     1621 tggtgagcag ttcgcgacgc tggcggccat cgccgccggt gggccgctgc cgtaaccgcg
     1681 ccgggggcat gccaaacggt cttccgaggg ctggacgaca cacgagtact cgtgcaagag
     1741 tagtcatgtg tcgtcaattc gcatcttcat tctggcggcg ctggaggagc gcgggccgat
     1801 gcacggccat cagttgcggc tgctcgccga agaggagcac gtgggcctgt ggacagacat
     1861 caccgtcggc gggttatacg gcgcgatcaa gcggctcgcc gccgaggatc tcatcgagga
     1921 gatccgggtc gagcgagccg gtgcctaccc ccaacgacag gtgtgggcgg tcacagaggc
     1981 tgggcgggag gccgttggcg cgctgcgcct gagggcgttg cgcgagattg tcgtcaagcc
     2041 ggaccctttc gacctggcga tcaccggggt gcatccggac catctcgatg atctgccgac
     2101 gatgatcgcg gctcgcatcg gatcgctgaa cgccatgctt gccgactggg aagcccatgc
     2161 cgccaccatc gaccgttatc tgaccgaggc cgaaaagttg gtgatgaggc accgggccga
     2221 ccggctgcgc gccgaaatcg cttggcatga ggaactttcc gaagctttac ccggaatcgc
     2281 tttgcaacag cgcgcgaggg ctgaacagac atgaccgcgc cgacaccgaa aacggttgtc
     2341 accgaacgcc gtaactggtt ggcgttggcc gtgctgctgg tgggcgcatt catggctttg
     2401 ctggacacca cgatcgtcaa tgtcgccatt cccaccattc gcgccagctt ggcggcctcg
     2461 aatgccacgc tgtcatggat catttcgggc tacgcgctgg ccttcgggct ggctctgata
     2521 cctgccggca gggtcgggga ccggctcggg cacaaatggg tgttcatcgc cggcttgtcg
     2581 ctgttcaccg ttgcgagcct ggcgtgcgga cttgcgacgg acgacatgca gctcgtttcc
     2641 gcgcgggcgg ttcagggcct ggccggtggg atgttcttca ctccgatcac cgcactgatc
     2701 cagctgatgt tcaccggccg cgagcgggcc cgagccttcg cggtgatggg agcgaccatc
     2761 ggtttttccg tcgccctcgg cccgctcgtc ggcgggttga tcattgcggc ctcgggcgca
     2821 gacgcgggct ggcggctggt tttcgacgtc aacgttccgt tgggcgtgct cgcggtcgtc
     2881 gccgcggtac tgctccttcc ggccggagcc gaaaatccgg ataccgcagc cgactggccc
     2941 ggtcttgtgt tgctgtcggc ggcactggtc gcgttgctga ccccgttgat ccaggggcag
     3001 caagagggct ggccattctg gacctacgcg tcgatggccg gcgccgtcgc gctgctcgcg
     3061 gtgtttgcgc tgtgggaacg ccacgtcgaa accaccggtg gcaatccgct ggtaccgccg
     3121 cgattgttcg tgcatgcctc gttcagcgga ggcgtgctgc tcgcgctcgt ctacttcgcg
     3181 gccttcacca gcatcttctt cacgatcgcg ctgctgtggc aggcgggtct gggccataca
     3241 gcgctgcaat ccgggctggt ggtgacgccc ttcgcggtcg ggttgatcgg cggtgcctcc
     3301 cgaagtgacg cgctggctga gcgcttcggc cgcgctgtca tcgtggctgg actgggcatg
     3361 gtcgccgtcg gtatcaccgc ggtgtggttt cttctcgcac ggatgccctc gaccggctat
     3421 agcggctggc aactggtcgc tccgctgttg attgccggcg tcggcagcgg gcttttcatc
     3481 gcgcccaacg ttgacttcat cgtcgctacg gtcgacccgc aagacgcggg cgcggccagc
     3541 ggtgtgatcg ggaccatgca acgtgtcggc agcgccatcg gcatcgcgat catcggcacg
     3601 attctcttcg gcaccctgca cgtcaaccgg gccggtcccg acgcggtcgc gcgggcgttc
     3661 ggtcatagcg ccacgctcgc catggccgcc agcgccggac tcagcatcgc ggccttcgcg
     3721 ctgggttttg ctttgccgaa acgaaccggc tgaccgccga gcgccggtta cggccgatca
     3781 accccgaacc ggtagggtgg cgcacatgca attggcgctg cagatcaccc tggtcgtcac
     3841 cagcatcctg gtggtgttgc tggtactgct gcaccgtgcc aagggtggcg gtctgtccac
     3901 gctgttcggt ggtggtgtgc agtccagcct gtcggggtcg accgtggtgg agaagaacct
     3961 ggaccggttg acggtgttca tcaccggcat ctggttggtg tgcatcatcg gcatgggttt
     4021 gttgatcaaa taccgttgag cagttcggcc gttcgtgcgg ccccgacagc cgaccacttg
     4081 ccacaagagg cggaccacgc gatccgcacc gatactggga cgcatggttg aggtttccga
     4141 caccacacta gaaccgatcg gcgccgtaca gcgcacccga atcggtcgcg aggcgaccga
     4201 gccgatgcgc gccgacatca ggctgctggg caccatcctg ggcaacaccg tgcgcgagca
     4261 gagcggtgac gaggtgttcg acctggtgga acgtgcgcgg gtggaatcgt ttcgggtgcg
     4321 gcgctccgag atcgaccggg ccgagatggc gcggatgttc gacggaatcg acatccacct
     4381 ggcgatcccg gtcattcggg cgttcagcca cttctcgctg ctggccaacg cggccgaaga
     4441 catccatcgg gagcgccggc gcgcgattca cgtcgacgcc ggcgagccgc cgcaggacag
     4501 cagcctcgcc gcgacctacg cgaagctcga tcttgcggaa ctggattcgc ccacggtggc
     4561 cggcgccctg cggggcgcgc tggtgtcgcc ggtgatcacc gcccatccca ccgagacacg
     4621 ccggcgcacc gtgttcgtca cgcagcaccg aatcaccgaa ttgatgcggc tgcacgccgc
     4681 gggacacacc gagaccgacg acggccgcag catcgaactc gaactacgcc gccaggtcct
     4741 cacgctctgg cagacggcgc tgatccggat gtcccggctg cagatcaccg acgagatcga
     4801 ggtggggctg cggtattacc cggcggcgct gttcagggtg atcccgcagg tcaatgccga
     4861 ggttcgcgac gcgttgcggg cccgctggcc cgatgccgac ctgctgtcgg cgcccatcct
     4921 gcaaccgggc tcctggatcg gcggcgaccg cgacggcaac ccgaacgtca ccgccgacgt
     4981 cgtgcgccgg gccaccggca gtgccgcgtt caccgcactg gcgcactacc tggccgaact
     5041 caccgacctc gagcaggagc tgtcgatgtc ggcccggcta atcaccgtca ccgggcaatt
     5101 gaccgcactc gagcagagct gcccggagaa agcgcgcgcg gacgagccct accggcgggc
     5161 ggtgcgggta atccgcggcc ggctcacggc gacggccgcc gacatcctgg actggcagcc
     5221 gcagcacgag cttgacctgg gtctgccgcc gtattccgcg ccggccgagc tgcgggccga
     5281 tctggacgtc gtcgacgctt cgctgcgcat gcacggggcc gcgctgttgg ccgacgatcg
     5341 gctggccaga ttgcgagaag gcgtgcatgt cttcggattt cacctgtccg gcctggacat
     5401 gcggcagaac tccgacgtgc acgaagaggt gatcggcgag ctgctcgcct gggccggggt
     5461 gcatccggac tacggctcgt tgcccgaaga cgaacgcgtc gagctgctga cgggcgaact
     5521 cggcacccgc cgtccgctgg tcggcgaccg tgcgcaattg tccgatctgg ctcgcagcga
     5581 gctgggcgtc gtcgcggcgg ccgcccaggc cgtcgagcgc tacggtccgg cggcggtgcc
     5641 caactacatc atctcgatgt gccagtcgtt gtcggacatc ttggaggccg cgatcctgct
     5701 gaaggaggcc ggactgctgg acgcgtccgg gccggaaccc tattgcccgg ttggcatctc
     5761 accgctgttc gagacgatcg aggacctgca caacggggcg tcgattctgc aggccatgct
     5821 ggaccttccg ctgtaccgat cgctcgtgga cgctcgcggt ggatgccagg aagtgatgct
     5881 gggctattcg gactccaaca aggacggcgg ctatatggcc gccaactggg cggtctaccg
     5941 ggccgagctg accctcgccg aagtcgctcg caagaccggt attcggttgc ggctcttcca
     6001 cggtcgcggc ggcaccgtcg gccgcggcgg cggccccagc taccaggcca tcctggcgca
     6061 gccgccggga gcggtgaacg gctcgctgcg cctcaccgag cagggcgagg tcatcgccgc
     6121 caagtacgcc gagccggaac tggcccggcg caatctggag agcttggtcg cggccaccct
     6181 ggagtcgacg ctgctcgacg tcgagggcct cggcgacgcc gccgaaccgg cctacgcggt
     6241 gctcgacgag atcgccatcc tggctcatcg cgcgtacgcc gaattggtac acgagacacc
     6301 gggtttcgtg gagtacttca aggcttccac accggtcagc gagatcgggt cgctgaacat
     6361 cggcagccgg ccaacctcac gcaaacccac cgcctcgatc gctgacctgc gcgccatccc
     6421 atgggtgctc gcctggagcc agtcgcgggt gatgctcccg ggttggtacg gcaccggatc
     6481 ggcgttcgag cagtggatcg cggcgggtcc cgagaccgcg gccgagcggg tggcgatact
     6541 gcacgagctc tacgagcggt ggccgttcct gcgaagcgtg ctgtcgaaca tggcacaggt
     6601 gctggccaag agcgatctgg gcctggcggc ccgctacgcc gagctggtac ccgatgaggc
     6661 gttgcggcac agggtgttcg acaagatcgt cgacgaacac cggcgcacca tcgccatgca
     6721 caagctgatc accggtcacg aagacctgct cgccgacaac ccgacgctgg cccgttcggt
     6781 gttcaaccgc ttcccgtacc tggagccgct gaaccacctg caggtggagc tgctccgccg
     6841 ctaccgttcg ggcgacgacg acgaactcgt gcagcgcggc atcctgctca ccatgaacgg
     6901 gttggccagc gcgcttcgca acagcgggta accctcggcc gcggggtacc aggggcctat
     6961 tccaccgtcc ggttgcgtag cctgtccagc acggtgcgga ccgcgtgctc ggtgacggac
     7021 aacggcgaca tcaccgtctc acccaccttc accatgtcgt cgatccgccc gacgatggcc
     7081 tccaccggct cgacgagtgc gatgagccgc ctggcgagtt catccaggtt ctgcagcgtg
     7141 ccctccagat gatccagacc gccctccagg cgttccaccg tggcgttgag ggcagacagc
     7201 gagctgctca gttctttcat cgttttgccg aggccgtcga ggacgtcttc gacctgctcc
     7261 accgtcttgt cggcattcaa cgcggcctgg gtaagggttc tgatccggtt acgctcggac
     7321 ccgctgcgcc cgcttctgtc ggccatgacc gtcattatga ctcgcgcgga acccgcgtgt
     7381 cgggggcccc gtggaggtca gccgggcagc ttggatgcgg cctgctcgtc gagcagccag
     7441 agcgtggtct gcagcccgat ggccccggcg gccggaaccg agaccggcga ggcgccaccg
     7501 atggcctcgg ccgccgcttc ggccttgccc gccccggata ccagcagcca cacctcgcgg
     7561 gaacgctgga ttgccggcag tgtcaaggtg attcgctgcg gcggcggctt gggggagtcc
     7621 tcgacggcga ccaccatgtg agtggtctca cgcacggccg gggtgtccgg gaacagggag
     7681 ttgatgtggc cctccggccc catgcccagc aggtggacgt cgaaattcgg cacccgttgg
     7741 ccgtcgtcgg cgttggcggc cagcaactgc tcgtaggcca gcgcggcggc cgcgaggtcg
     7801 ctgccgaact cgccatcgct ggccggcatc gtgtgcacgt ggctcgacgg gatgttgatg
     7861 tggtcgagca aggctgcccg agcctgcttg tcgttgcgct cgtcgtcgtc ctcgggaacg
     7921 tagcgttcgt caccccagaa caggtgcacc ttggaccagt cgatcagctg acggtgcctg
     7981 ccgaggtact gcagcagccc gatgccgttg ccgccgccgg tcagcacgat cagggctcgc
     8041 ccgcgggctg ccacggcggt cttgatggtc tcgaccagtc gcccgcccgc ggcgtcgacc
     8101 aaggcctcgg agtccgcgaa aacctcgaca actttgctca cacgtactgc accttcttta
     8161 tgccttcgag ggcggtcaag tagatgtcgt cggcattgag tctgcgcagg tcctcggcca
     8221 ggcattcacc ggtttccctg cgcgccaacg gaaccaacgc ttccggcttg gccgtgcggc
     8281 tcagggttgc ggtcctgcct tcctggggcc gggttaaggc gatggtctcg ctcttgcgtg
     8341 ccaactcgac cttgagttcg cccaccgcac gacgcaccgg gccgtcgatc cggctggcca
     8401 gccagcccgc caggatgtcc agcgcgggtt cgttcttcag gccggagacc agcgccgact
     8461 cgatcggctc gtggggcggc tggtcgacgg cggaggtcag cagcgcccgc cagtaggtga
     8521 tgcgtgccca gcacagatcg gtgtcaccgg cggtgtatcc gggcagtcgg cccttgatgg
     8581 ccaacaacgg atcggggccg ttggtggcat cggtgatacg ccgaatcgcc agcttgccca
     8641 agggatcctg ggcgggcacc gccggggcga cgtcaggcca ccacgccacc accgggatgt
     8701 cggggagcag aaacgggatg accacgctcg aggcgtggcc ggcgagcggc ccggacagcc
     8761 gtagcaccac gatctcaccg gcgccggcgt cgccgcccgc acgcagttgt gcgtccaagc
     8821 gcgcgtcctc ggcgtgcgga tcgcctgtca tcgtgacgat gatccggctg ggatgctcgt
     8881 ggctggcatc gttggcggcc ttgagcgatt gctccagcac ctcttcgctg tccggcgcga
     8941 tgatcagcgt cagcacgcgt cccatcgtga cggcgccgat cttttcgcgt agctcgtcga
     9001 gtttcttgtt gacggccgtg gtggtggtgc cgggcagatc gacgatcatt tgcgccactc
     9061 tcccccgcaa gcgggcggta cccccacagc atcgccggcg cggatcatgg ccgccgccat
     9121 tcccggccgg tgcggcgcag catctcgaag gatgactcgg ggccccaggt tccggattca
     9181 taggggtcgg gcttaccgtg tgcggcccag ttctccagga cgggatcgag tatttcccaa
     9241 gccaattcga cctccgcgtt aaccggaaac agggatggct cgccgagcag tacgtcaagg
     9301 atcagcgctt cgtaggcctc cggcgattcc tcggcgaacg ccgatccgta ggagaagtcc
     9361 atgttgacgt cgcggacctc catggcggtg ccgggcacct tggagccgaa ccgcagggtg
     9421 atcccttcgt ccggctgcac gcggatgacg agggcgttgg cgcccagctc gtcggtcatc
     9481 gtggcgtcga atggcagatg cggcgcgcgt ttgaagacca gggcgatttc ggtcaccctg
     9541 cggcccaggc gtttacccgt tcgcaggtag aacggcacac cggcccagcg gcgggtgtcg
     9601 acctcgagcg tgatggcggc aaaagtctcc gtggtggagt ccttggcaaa accttcttcg
     9661 tcgagcagcc cgacgacttt ctcgccgccc tgccaaccgg ccgcgtattg gccgcggctg
     9721 ctggtctcat cgagcggcag cgcgagttgc gtcgccgaaa gcaccttgat cttttccgtt
     9781 tgcagcgcct tcggatggaa gctcaccggc tcttccatcg cggtcagcgc cagcagctgc
     9841 atcagatggt tctggatgac gtcgcgggcg gcgccgatgc cgtcgtagta gccggcccgt
     9901 ccacccagcc cgatgtcttc ggccatggtg atctgcacgt ggtcgacgta gtgggcgttc
     9961 cagaccgggt cgaacaactg gttggcgaag cgcagcgcca ggatgtttcg caccgtctct
    10021 ttgcccaggt agtggtcgat ccggaatacc gactcctccg ggaagaccga gttgaccacc
    10081 tggttgagat cgcatgcgct ttgcaggtcg tggccgaagg gcttctcgat gacgacccgg
    10141 ctccatctgt cctcttgtgg acgggccagt ccggacttgt gcagttgttc acataccacg
    10201 gggaacgact tgggcgggat cgccaggtag aagacgtggt tgccgccgat gccgcgctcg
    10261 gcatcgagct tatccagcgt ctcgccgagc cgggcgaacg cggcgtcgtc gtcgaactcg
    10321 cctcgcacga aacgcaatcc gtccgccagc cgttcccata ccgcttggcg gaacggggta
    10381 cggcagtgcc ctttgacgtc atcgtgtatt acctgggcga aactgtcgtg atcccagtcg
    10441 cggcgggcga aacccaccag ggagaaggtg ggtggcaaca agccgcggtt ggcgaggtcg
    10501 taaatcgccg gcaccacctt tttatgggcc aggtcgccgg tgacaccgaa aagcaccagg
    10561 gcacatggac cggcgatcct gggcagccgc ttgtcctttt tgtcccgcag cgggttctgc
    10621 cactccgcga cggcgccgcg ggtgcccgcc ttcgagtttg ccgggctcat ttggtggcgg
    10681 aacccagttg cttctgcgtt tcccctagca actcggtcca ggactccacg aacttgtcca
    10741 cgccctcttt ttccagcacg acgaagacat cggtgaggtc gatgccgacc gccgtcagct
    10801 tgtcgaacac ctcctgggca gccgcggcgg tgccgctgat cgtgtcgccg gtgacgacgc
    10861 cgtggtcggc gaccgcgtca attgtcttct ccggcatggt gttcaccgtg tgcgccgcga
    10921 ccagctcggt gacgtaaaga gtgtcggagt aggccggatt cttgacgccg gtcgaagccc
    10981 acagcggacg ctgtacccgg gccccgtcgg acttgagtgc cccgtagcga tcgccgtcgt
    11041 cgaagacatc ctggtaggcg gcgtaggcca gccgggcgtt ggcgacgccg gccttgccgc
    11101 gcaacccgag ggcttcctcc gagccgatct tctccagccg cttgtcgatc tcggtgtcca
    11161 cgcgggagac gaaaaacgat gcgaccgaat ggatcttgga caggtcgtgg ccggcctggc
    11221 gggccttttc cagccccgcc aggtaggcgt ccatcaccag gcggtgccgt tcgacggaga
    11281 agatcaacgt gacgttgacc gaaatccctt ccgccagaac ggcggtgatg gccgggagtc
    11341 cggccttggt cgccgggatc ttgatgaaca ggttggggcg gtcgacgatt ttccacagct
    11401 cgacggcctg ggcgatcgtc ttgtcggtct cctccgccag ccgcgggtcg acctcgatgg
    11461 acacccggcc gtcgactccg tcggaggcct cccactgggg agccagcacg tcgcaggcgg
    11521 tgcgcacgtc atcggtcgta acggtgcgca cggtcgcgtc gacgtcggcg ccgcgttcgg
    11581 ccagctcggc gatctggccc tcgtaggcgt caccctcggc gaacgccttc tggaagatgg
    11641 atgggttggt ggtcacgccg acaacacttt tggtgtcgat cagctcctgc aagttgcccg
    11701 actgcagccg ctcccgcgac aagtcgtcca gccaaacgga taccccagcg gcgctcagcg
    11761 ccgcgaggtt agggttctga ctggtcatcg gaatcaccct ttctcagttg tccactactt
    11821 gttccgcggc ggcagcgacg gcttcagcgg tgaagccgaa ctcacggaac aaagtcttgg
    11881 cgtcggcgga ttcgccgtag tgctcgatcg acacgatctt tccggtgtcg ccgacaagct
    11941 tgtgccacga ctgcgcgatg ccggcctcga cggccacccg ggccgacacc gacggcggta
    12001 acacgctgtc gcagtagtcg gacggctggg actcgaacca ctccacgcac ggcatcgaca
    12061 ccactcgcgc gacgatgtcc ctgtccgcca acaggttttg ggcttcgacc gcgagctgta
    12121 cctcggaacc ggtggcgatc aggacgacgt cgggctcgtc cccgccgtca tcacccagca
    12181 cgtatccacc acgggcgacg ccctcgatgt tggtgccttc cagcaccggc agaccttggc
    12241 gcgtcaggat caggccaacc ggaccgctcc cgttgccgcg ggccaggatc gttcgccacg
    12301 cgtaggccgt ctcgttggcg tcggccggac gcaccaccga caagtgcgga atggcccgca
    12361 gcgccgaaag gtgctcgatc ggctgatgcg tgggaccgtc ctcgcccaac ccgatcgaat
    12421 cgtgcgtcca cacgtagatc gtgtcgatgt tcatcagcga ggccagccgc accgacgggc
    12481 gcatgtaat
//
LOCUS       DASSGQ010000123         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_829477,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000123 DASSGQ010000000
VERSION     DASSGQ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829477"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VFI55_05780"
     CDS             complement(<1..751)
                     /locus_tag="VFI55_05780"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HET7073577.1"
                     /translation="MLTAIGMAGTPAALASAQSGLQAVAAPPVNVDVSQRHLNESEEA
                     IAVNPVNPSNIVTVTNVGHAEAGLTAGMFEGVSFDGGKTWTRKLIALGGGDPLGDGCC
                     DPSLSFDSHGNLFMTYLYEVEDQVPVALSTDGGVSFHVIANISQASLPAKKSGGDNRG
                     LFRFVDQPTITAAHNEVWVVFNAGGPMAATGAAVTGLGKVGNFGQVEVVPGTNNCTYG
                     DVAIGPSGQVMQACSLTESGQGGGKIFVNVDP"
     gene            complement(925..>2212)
                     /locus_tag="VFI55_05785"
     CDS             complement(925..>2212)
                     /locus_tag="VFI55_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073578.1"
                     /translation="PVNGTFSYANGTRLYYSNLATNLTDTKIEQGGINADTAITVSHI
                     DNLTPARVADQKNWSKPYFVGAHISAASGLDKEQVWADNAASSPFFGNVYVCFSDFHS
                     LSQGNNFALFPSVAVSSDGGVTWKTHHVAPPTTAAPQGFRQGCTVRTDSHGVVYDFIT
                     HFSNTSNNGAQTLVTSSTGGANWNRPVDFMQMNASCFFFDPVAGRCNSEGPAGARNDL
                     MAMPSVDIANGAPAGAGATNEIVDVWSDGRLGLNHEVALLSFSTDGGQSWSAPATVSA
                     PGDRALFSAPGIAPDGSRVYLAYNAFTTPFANTTGTPRLEHGVLRSAAIGAGGAPTGW
                     ATEFVGASGDARGTSQGRILYNEFLGDYVYAIGTRTYGAGVWTDVPNTADCPAMDAWR
                     QASFTAGHRVFPAPWPLGDCPANFGNNDISGATTAP"
BASE COUNT          339 a          734 c          772 g          367 t
ORIGIN      
        1 cggggtccac gttcacgaat atcttgccgc cgccctgccc ggactcggtc aggctgcagg
       61 cctgcatcac ctggccggac ggtccgatgg cgacatcgcc gtaggtgcag ttgttcgtcc
      121 cgggcaccac ctcgacctgg ccgaaattac cgaccttgcc gagtccggtg accgcggcgc
      181 cggtggcggc catcgggccg cccgcgttga acaccaccca gacctcgtta tgcgccgccg
      241 tgatggtggg ctggtcgacg aagcggaaca gcccgcggtt atccccgccc gacttcttgg
      301 cgggcaggct ggcctgggag atgttggcga tcacgtggaa gctgacgccg ccgtcggtgc
      361 tgagcgcgac cgggacctga tcctccacct cgtacaggta ggtcatgaac aagttcccgt
      421 ggctgtcgaa ggacaggctc gggtcgcagc atccatcgcc gagcgggtca ccgccgccca
      481 gggcgatcag cttccgcgtc cacgtcttgc cgccgtcgaa gctgacgccc tcgaacatcc
      541 cggcggtgag cccggcctcc gcatggccca cattggtcac cgtcacgatg ttgctggggt
      601 tgaccgggtt gacggcgatc gcctcttcgg actcgttcag atgccgctgg ctgacatcga
      661 cgttgaccgg cggcgcggcc acggcctgca gcccggactg agccgaagcc aacgcggccg
      721 gggtgcccgc catcccgatg gctgtcagca cggcaactac cactatcggg agccggaagc
      781 ggcacatgtt cagtccttcc cgtaaggcgc ctggccagag gctgcacctg gcatcgttga
      841 ccgaagagag ctcatgcatc cacgcgaaac cggcatctgg agcagagtcc agttcgcctg
      901 gcccggcgca tgcggacgca tcgtctaggg cgctgtcgtg gcgccagaga tgtcgttgtt
      961 gccgaagttc gccgggcagt cccctagcgg ccacggcgcc gggaacaccc ggtggccggc
     1021 ggtgaaggag gcctgccgcc atgcgtccat ggccgggcag tcggcggtgt tcgggacgtc
     1081 ggtccacacg cccgctccgt aggtgcgggt gccgatggcg tagacgtagt cacccaggaa
     1141 ctcgttgtac aggatccggc cctgggaggt accccgagcg tcaccgctgg cgccgacgaa
     1201 ctcggtcgcc catccggtcg gggcgccgcc cgcgccgatc gcggccgacc gcaggacccc
     1261 gtgttccagc cgcggggtac ccgtcgtgtt ggcaaacggc gtggtgaaag cgttgtaggc
     1321 gaggtacacg cgggacccgt cgggggcgat gcccggcgcc gagaacagcg cccggtcgcc
     1381 cggcgcggac acggtcgccg gcgcggacca gctctggccg ccgtccgtcg agaacgacag
     1441 cagcgccacc tcgtggttca ggccgagccg gccgtcgctc cacacatcga cgatctcgtt
     1501 ggtggcacct gccccggcgg gagcaccgtt ggcgatgtcg acgctgggca tggccatcag
     1561 gtcattccgc gcgcccgccg gtccctcact gttgcagcgg cccgcgaccg ggtcgaagaa
     1621 gaagcaggag gcgttcatct gcatgaaatc gaccggccgg ttccagttgg cgccgcccgt
     1681 cgacgaagtc accagggtct gcgcgccgtt gttcgacgtg ttggagaagt gcgtgatgaa
     1741 gtcgtagacg accccgtggc tgtccgtgcg gacggtgcac ccctgccgga agccctgcgg
     1801 agccgcagtg gtgggcgggg cgacgtggtg cgtcttccag gtgacgccgc cgtcagagga
     1861 cacggccacc gacgggaaca aggcgaagtt gttgccctgg ctcaggctgt ggaaatcgct
     1921 gaagcatacg tacacgttgc cgaagaacgg gctgctggcc gcgttgtccg cccagacctg
     1981 ctccttgtcc aggcccgacg cggcggaaat gtgcgcgccc acgaagtacg gtttcgacca
     2041 gttcttctgg tcggccacgc gggccggcgt caggttgtcg atgtgcgaga ccgtgatggc
     2101 ggtatcggcg ttgatgccgc cctgctcgat cttggtgtcg gtcaggttcg tggccaggtt
     2161 ggagtagtac agccgggtgc cgttggcgta ggagaacgtc ccgttcacgg gc
//
LOCUS       DASSGQ010000124        10737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1011634,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000124 DASSGQ010000000
VERSION     DASSGQ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10737
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011634"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFI55_05790"
     CDS             <1..246
                     /locus_tag="VFI55_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073579.1"
                     /translation="DWEAAEEAPQSHRSYASGLVAAHLQWHLERQLKTLPLVERPYQA
                     DRASPSDQNPHGQDPHGRNPHIAERRAAPIGQDTACG"
     gene            248..1159
                     /locus_tag="VFI55_05795"
     CDS             248..1159
                     /locus_tag="VFI55_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl diphosphate synthase"
                     /protein_id="HET7073580.1"
                     /translation="MRFGDSGRKADRKLKSTTFPQQLPPAPADYPVFPDMSTWPVVFP
                     DLPPSPGGGPSRPPQHVSKAAAPRIAADRLPFHVAIVMDGNGRWATQRGLPRVEGHKM
                     GEAVVIDVVCGAIELGIKWLSLYAFSTENWKRSPEEVRFLMGFNRDVVRRRRENLKAM
                     GVRIRWVGSRPRLWRSVINELAIAEAMTADNDVITINYCVNYGGRTEIAEATRNIAQL
                     AVAGKLDPERITESTIARHLQRPDIPDVDLLLRTSGEQRSSNFMLWQAAYAEYIFQEK
                     LWPDYDRRDLWAACEEYSARNRRFGSA"
     gene            1159..1587
                     /locus_tag="VFI55_05800"
     CDS             1159..1587
                     /locus_tag="VFI55_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073581.1"
                     /translation="MPSLQERLASILSDALPVEQEPDGALTLRHNGTLASLRVVNIAE
                     DLDLVSLTQILAWDLPLTKKVGDQVAKQAHDSNFGSVTLVEKVNEKAVRRNSGKSASK
                     TADVMLRYNFPGGGLTDGALRTLILLVLDTGADIRRTLIG"
     gene            complement(1620..2012)
                     /locus_tag="VFI55_05805"
     CDS             complement(1620..2012)
                     /locus_tag="VFI55_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET7073582.1"
                     /translation="MTGTSVRSTRQRAAISTLLETLDEFRSAQELHDELRRRGENIGL
                     TTVYRTLQSMATAGMVDTLRTDTGESVYRRCSDHHHHHLVCRGCGSTIEVGDREVEEW
                     AAQVAAKHGYSDVSHTIEIFGTCSQCAS"
     gene            complement(2009..2419)
                     /locus_tag="VFI55_05810"
     CDS             complement(2009..2419)
                     /locus_tag="VFI55_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013806012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7073583.1"
                     /translation="MPSPGPTTADGDAGSDLSVAEHDHGGVGSGEHSAFPEYPVPPSR
                     EILDAAGELLRALAAPVRIAIVLQLRESHRCVHELVDALGVPQPLVSQHLKILKAAGV
                     VTGERSGREVLYRLADHHLAHIVVDAVAHAGEGV"
     gene            2602..4017
                     /locus_tag="VFI55_05815"
     CDS             2602..4017
                     /locus_tag="VFI55_05815"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003915261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HET7073584.1"
                     /translation="MFARSGKGVHHPVASVIDTVVNLAKRRGFVYPSGEIYGGTKSAW
                     DYGPLGTELKENVKRQWWRSVVTGRDDVVGIDSSIILPRQVWVASGHVEVFHDPLVES
                     MITHKRYRADHLIEAYEAKHGHPPPNGLAGIRDPDTGEPGQWTEPREFNMMLKTYLGP
                     IETEDGLHYLRPETAQGIFVNFANVVTTARRKPPFGIGQIGKSFRNEITPGNFIFRTR
                     EFEQMEMEFFVEPSTAREWHQYWIDTRLQWYVDLGIDPENLRLFEHPKDKLSHYSDRT
                     VDIEYKFGFVGNPWGELEGVANRTDFDLSTHAKHSGVDLSFYDQVTDIRYTPYVIEPA
                     AGLTRSFMAFLIDAYHEDEAPNAKGGLDKRTVLRLDPRLAPVKAAVLPLSRHADLSPK
                     ARDLAAELRKCWNIDFDDAGAIGRRYRRQDEVGTPFCVTVDFDSLEDDSVTVRERDEM
                     TQDRVAMSAVADYLAVRLKGC"
     gene            4144..6000
                     /locus_tag="VFI55_05820"
     CDS             4144..6000
                     /locus_tag="VFI55_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPE domain-containing protein"
                     /protein_id="HET7073585.1"
                     /translation="MMSFLVLPPEINSLRMFSGAGSDPMLSAAAAWDGLAAELGSAAQ
                     SFSSVTSGLTAGPGQAWQGAASAAMVAAAAPYAVFLSAAAAQASGTASQARAAASVFE
                     SALAATVHPVAVAANRSDLVSLVTSNLFGQNAPAIAAVESDYEQMWAADAAAMAGYHA
                     GASAVAAQLSSWSAVLPGVPGLPGLVGGAQSAGAQAAASVAAFPSVNVGLGNLGSYNL
                     GSGNIGNLNLGSGNSSGRFNLGSGNFGGGNLGSGNIGTANVGSGNIGDNNIGGGNFGG
                     GNGGSGNIGSFNSGFGNNGAFNFGSGNIGNGCFGGGNNGSFNFGLGNFGGGNVGFGNN
                     GSNNIGFGNTGNGNIGIGLTGNGQIGIGALNSGIGNVGFGNSGTNNIGFFNSGNGNFG
                     IGNSGNGNWGYGNAGSINTGFWNSGSTNTGFGSAGSGNFGFYDAGVGNSGGFSAGYEN
                     SGFWNSGAGDTGFWNSGNSNTGFWNSGDTNTGLFNSGNINTGGFNAGTLNTGYFSAAT
                     QNAANSGFANLGTDNSGFGQNNSAGSGNSGFQNSGFGNSGFTNTSTMSGNPGNSGILN
                     TGIGNSGYQNAAVEDAGIFVSGVMSSGVFTSGTGSSGIFVTGNGLSGFPSLF"
     gene            6332..7519
                     /locus_tag="VFI55_05825"
     CDS             6332..7519
                     /locus_tag="VFI55_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003900505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPE family protein"
                     /protein_id="HET7073586.1"
                     /translation="MVPAFTFLPPEINSALMYSGAGSGPLFTAASAWDGLASDLSGAA
                     SSFQSVINGLAGGPWSGPASASMVAAATPYVGWLSAAAIQAEAAAGQARAAATAFESA
                     LAGTVPTSAVTANRVQLMTLIATNILGQNTPAIAMTEFEYMEMWAQDVAAMVGYHVGA
                     TGVAAALPSFSVPPAGLAGLGGLLASPLVGVASEVSGVASSAGGVLASGVTSAVGPAV
                     AGVSTVASALPVSEISQVAQIAMYPASMMMSPIMMAAQAGMGAHPALAGADMAAAGPK
                     FVSDAVPKPLGGAGLGGLGGAAGGLGKARLVGAMSVPPTWQGAAPSRMVSSAMSGLGS
                     ELAGAAAPAGAPMGGGMPMMPMPMGGMGGGGMPGGMLGRGGASPNHVVQSRPTVVPRT
                     GVG"
     gene            7910..9466
                     /locus_tag="VFI55_05830"
     CDS             7910..9466
                     /locus_tag="VFI55_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020786705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase C"
                     /protein_id="HET7073587.1"
                     /translation="MSRNPVDGMSRREFFSKVAAATSAGAVMSLAAPVIEKAYGAGPC
                     SGHLTDIEHFVLLMQENRSFDHYFGTLSGTDGFDTASAAFQQKGWNPMTQTLDPNGVT
                     IPYRFDTTRGPLQDGECVNDPGHDWITMHGAWNNGNNNGWLPAQANESPLVGNVPVTM
                     GYYTRRDLPVHYLLADTFTICDQYHCSLLGGTTPNRLYWMSATIDPDGTHGGPQLVEP
                     NVQPIQRFSWTIMPENLSAAGVSWKVYQNKLLGALNNTVIGYNGLVNDFKQSANPQSD
                     LARNGIAPTYPGNFVSDVRNNTLPAVSWILPGFLYSEHPSFPVCVGAVAIVDVLRTLL
                     SNPAVWEKTALIVTYDENGGFFDHVTPPTPPPGTPGEFVTVPDIDAVPGSGGIRGPIG
                     LGFRVPTFVISPYSRGPLKCSTVLDHTSQLKLLRTRFGVPVPNLSPWRDQTVGDMTTA
                     FNFAVPPNPSRPSLDHPVLAALPKLPQCVPNAVLGSTVKTSIPYRVPYPQSMPTQETA
                     PLRGTPSGIC"
     gene            10141..10467
                     /locus_tag="VFI55_05835"
     CDS             10141..10467
                     /locus_tag="VFI55_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015575189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073588.1"
                     /translation="MLLPLGSPLPDDSVAAVRGTSGMLGGLSVPLKWGVVVPPDNYDH
                     WAPEPEEGVSAGDDVADGLAPEATAEGMDEWNEWGEWSGWEGEAEPRFDVPRTSSVIP
                     HSPAAG"
     gene            10593..>10737
                     /locus_tag="VFI55_05840"
     CDS             10593..>10737
                     /locus_tag="VFI55_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HET7073589.1"
                     /translation="MARFMTDPHEMRAMAGRFEMHAQTVEDEARKMWASSMNIAGAGW
                     SGQA"
BASE COUNT         1859 a         3311 c         3666 g         1901 t
ORIGIN      
        1 gattgggagg ccgccgaaga ggcgccgcag tcgcaccgca gctacgccag cggattggtg
       61 gccgcgcatc tccaatggca tctggaacgg cagctcaaaa cattgccgtt ggtagagcgg
      121 ccgtatcagg ccgaccgcgc ctccccgagc gaccagaacc cccacgggca ggacccccac
      181 gggcggaacc ctcacattgc cgaacggcgc gcagcgccga tcgggcagga tacggcttgt
      241 ggctaacatc cggttcggcg actcgggacg caaagccgac cgcaagctga agtcgaccac
      301 cttcccgcag cagctgcccc ccgcgcccgc cgactacccg gtctttcccg acatgtcgac
      361 atggcctgtc gtcttcccgg atctgccgcc gtcccccggc ggtggcccga gccgcccgcc
      421 acagcacgtt tcgaaagcgg cggcacccag gatcgcggcc gaccgattgc ctttccacgt
      481 cgccatcgtg atggacggca acggtcgctg ggccacccaa cgcgggctgc cccgggtcga
      541 gggtcacaag atgggcgagg cggtggtgat cgacgtcgtc tgcggtgcaa tcgaactcgg
      601 gatcaaatgg ctgagcctct acgcgttttc gaccgaaaac tggaagcgtt cccccgaaga
      661 ggtccggttc ctgatggggt tcaaccgcga cgtggtgcgg cgccgccgcg agaacctcaa
      721 ggcgatggga gtccggatcc ggtgggtggg ttcgcgacca cgcctgtggc gcagcgtcat
      781 caatgagttg gcgatcgccg aggcgatgac ggcggacaac gacgtcatca cgatcaatta
      841 ctgcgtcaac tacggtggcc gcaccgaaat cgcagaagcc acaaggaata tcgcccaatt
      901 ggcggtggcg ggcaagctcg acccggaacg gatcaccgag tcgacaatcg cgcgtcacct
      961 gcagcggccg gacatccccg atgtggacct gttgctgcgg acctcggggg agcagcggtc
     1021 gagcaatttc atgttgtggc aggcggcgta tgccgagtac atcttccagg aaaagctgtg
     1081 gcccgactac gaccgccgcg atctgtgggc cgcctgcgag gaatactcgg cccgtaatcg
     1141 aaggttcggg agtgcctagt gccgtcactg caagagcgtc tggcatcgat actcagcgac
     1201 gcgcttcccg tcgagcagga acccgacggt gcgttgaccc tgcgccataa cggcacgctg
     1261 gcgtcgttgc gggtggtgaa catcgccgag gatcttgacc tggtatcgct cacccagata
     1321 ttggcctggg acctgccgct gaccaagaag gtcggcgacc aggtcgccaa gcaggcgcac
     1381 gacagcaatt tcggcagcgt gaccctggtc gagaaggtca acgagaaggc ggtgcgccgc
     1441 aactccggga aatcggcgtc caaaaccgcg gatgtgatgc tgcgctacaa ctttcccggt
     1501 ggcggcttga ccgacggcgc cctgcgcacg ctgatcctgt tggtcctgga cacgggcgcc
     1561 gacatacgtc gcacgctgat tggttgatct cgccgccaag actcgcgtcg ggtcccgtgc
     1621 taggacgcgc actgcgagca ggtaccgaag atctcgatgg tgtggctgac gtcggagtag
     1681 ccgtgtttgg cggccacttg cgccgcccac tcctcgacct cgcggtcacc cacctcgatg
     1741 gtggaaccgc agccgcggca taccaggtgg tgatgatgat ggtccgagca tctgcggtag
     1801 accgactcgc cggtgtcggt acgcagcgtg tcgaccattc ccgccgtcgc catcgactgc
     1861 agcgtccggt agacggtggt gagcccgatg ttctcgccgc ggcgcctcag ctcgtcgtgc
     1921 agttcctggg ccgagcggaa ttcgtcgagt gtctccaaca gagtggaaat cgcggctcgc
     1981 tgacgggtgg aacgcacgct ggtgccggtc atacgccctc gccggcatgg gcgacagcgt
     2041 cgacgacgat gtgggccagg tggtgatcgg caagccgata gagcacctcg cggccggatc
     2101 gctccccggt gaccacgccc gccgccttga ggatcttcag atgctggctg accagcggtt
     2161 gcggcacgcc gagcgcgtcg accaattcgt gtacgcagcg gtgggactcg cgcaactgca
     2221 gcacaatggc gatccgcacg ggcgcggcca gcgcgcgcag caactcgccg gcggcgtcga
     2281 gtatctcccg cgacggcggg acgggatatt cggggaacgc ggagtgttcg cccgacccaa
     2341 ccccgccatg gtcgtgttct gcgacgctga ggtccgatcc cgcgtcgccg tcggcggtgg
     2401 tcggccctgg agacggcatc ggggaggaca tcaccatggg agcgtcatca cctcgagaag
     2461 tgccgggcat tgggaagtgc ccgccgttgg aaccacgatc ggctgccttc cactgtcaca
     2521 tgcatgatga cgcatgtcaa agaaccgcgg cccggtcgtt accatgactg atgttttgca
     2581 ggtagcggtg cgtcgctgcc cctgttcgcc cgatccggaa agggagtaca ccaccccgtg
     2641 gcgtccgtca tcgacaccgt agtgaacctg gccaaacggc ggggcttcgt ctatccctct
     2701 ggcgagatct acggcggcac aaaatccgcg tgggattacg gcccgctggg caccgaactg
     2761 aaggagaacg tcaaacggca gtggtggcgt tcggtggtca ccggccgcga cgacgtcgtg
     2821 ggaatcgatt cgtcgatcat cctgccccgg caggtgtggg tggcctccgg ccacgtcgag
     2881 gtcttccacg acccactggt cgagtcgatg atcacccaca agcgctaccg tgccgaccat
     2941 ctcatcgagg cctacgaagc caaacacggg cacccaccgc ctaacgggct ggccggcatc
     3001 cgcgaccccg acaccggcga acccgggcag tggaccgagc cccgcgagtt caacatgatg
     3061 ctcaagacct acctcgggcc catcgaaacc gaggacggcc tgcactatct gcggccggag
     3121 accgcgcagg gcatcttcgt caacttcgcc aacgtggtca ccaccgcgcg ccgcaagccg
     3181 cccttcggga tcgggcagat cggcaagagc ttccgcaacg agatcacgcc gggcaacttc
     3241 atcttccgga cccgcgagtt cgaacagatg gagatggagt tcttcgtcga accgtcgacc
     3301 gcccgggagt ggcaccaata ctggatcgac acccggctgc agtggtacgt cgacctgggc
     3361 atcgatccgg agaacctgcg gctgttcgag caccccaagg acaagttgtc gcactactcc
     3421 gaccgcaccg tcgacatcga gtacaagttc ggcttcgtcg gcaacccgtg gggcgagctg
     3481 gaaggtgtgg ccaaccgaac cgacttcgac ctgtcgacgc acgccaagca ttccggcgtc
     3541 gacctgtcgt tctacgacca ggtcaccgac attcggtaca cgccgtatgt catcgaaccg
     3601 gccgccggcc tgacgcggtc gttcatggcg ttcctgatcg acgcctacca cgaggacgaa
     3661 gcgccaaatg ccaagggcgg cttggataag cggaccgtgc tccggctcga ccctcggctg
     3721 gcaccggtca aggccgcggt gctgccgctg tcccggcacg ccgacctgag ccccaaggct
     3781 cgcgacctgg ccgctgaatt gcggaaatgc tggaacatcg atttcgacga cgcgggcgcc
     3841 atcggccggc gctaccggcg gcaagacgag gtcggtacgc ctttctgcgt gacggtcgac
     3901 ttcgactcac tcgaggacga cagcgtcacc gtgcgggagc gcgacgagat gacccaggac
     3961 cgggtggcga tgagcgccgt cgccgattac ctggcggtgc ggctcaaggg ctgctgacgc
     4021 cgccgcgccg ggggcgtggt gtcgggtggt ttcgcgtgct tctgggtgct cccgggtgct
     4081 ccggtagccg ctgtcggtaa cgggtgggtg cgcggtggtg aattaaccgg tggaggaagg
     4141 gcgctgatga gctttttggt gttgccgccg gagatcaact cgctgcggat gttttcgggt
     4201 gccgggtccg atccgatgct gtcggctgcg gccgcgtggg acggcttggc cgccgagttg
     4261 gggtcggcgg cgcagtcgtt ctcgtctgtg acgtcgggtc tgacggctgg gccgggtcag
     4321 gcctggcagg gtgcggcgtc ggcggcgatg gttgccgcgg cggcgcccta tgcggtgttt
     4381 ttgagtgcgg cggccgctca ggcgtcgggg acggcaagtc aggcgcgggc ggcggccagt
     4441 gtgttcgagt cggcgctggc ggccacggtg catccggtcg cggtggcggc caaccggtcg
     4501 gacttggtgt cgttggtgac gtcgaacctg ttcgggcaga acgcgccggc gattgcggcg
     4561 gtggagtccg actacgagca gatgtgggcc gcggatgcgg cggcgatggc ggggtatcac
     4621 gcgggggcat cggcggtggc cgcgcagttg tcgtcctggt ccgcggtgct gccaggcgtg
     4681 ccgggtctgc cgggtttggt ggggggagcc cagtcggccg gtgcacaagc cgccgcgtcg
     4741 gtggccgcct tccccagcgt caacgtgggc ctgggcaacc tcggctccta caacctgggc
     4801 agcggcaaca tcggcaactt gaacctcggc agcggaaact catcgggccg gttcaacctg
     4861 ggcagcggca acttcggcgg cggaaatctc ggcagcggaa acatcggcac cgcaaacgtc
     4921 ggcagcggaa acatcggtga caacaacatc ggcggcggaa acttcggcgg cgggaacggc
     4981 ggcagcggaa acatcggctc cttcaactcc ggcttcggca acaacggcgc cttcaacttc
     5041 ggcagcggca acatcggcaa cggatgcttc ggcggcggga ataacggctc gttcaacttc
     5101 ggcctcggga atttcggcgg tggcaacgtc ggcttcggga acaacggcag caacaacatc
     5161 ggtttcggca acacggggaa tggaaacatc ggcatcggcc tcaccggcaa cggccagatc
     5221 ggcatcggcg ccctcaactc gggcatcggc aatgtcggct tcggcaactc cggtaccaac
     5281 aacatcggct tcttcaactc cggtaacgga aacttcggca tcggaaactc cggcaacggg
     5341 aactggggct acggcaacgc ggggagcatc aacaccggtt tctggaattc gggcagcacc
     5401 aacacgggct tcggcagtgc aggctcaggc aacttcgggt tttacgacgc gggcgtcggc
     5461 aattccgggg gcttcagcgc aggctacgag aactcgggct tctggaattc gggcgcggga
     5521 gacaccggtt tctggaattc gggcaactcg aatacgggtt tctggaattc gggtgacacc
     5581 aacaccggcc tgttcaattc gggcaacatc aacaccggcg gattcaatgc cggcaccctc
     5641 aacacgggct acttcagcgc ggcaactcaa aatgcggcga actcgggctt cgccaacctc
     5701 ggcaccgaca attcgggctt cggccagaac aactcggccg gcagcgggaa ctccggcttt
     5761 cagaactcgg gtttcggtaa ctcgggcttc accaatacca gcacgatgag tggtaacccg
     5821 ggcaactcgg ggatcctcaa cacgggcatt gggaactcgg gttaccagaa cgcggccgtc
     5881 gaagacgccg gcatctttgt ctcaggcgtc atgagttcgg gggtgttcac gtcggggacg
     5941 ggcagttcgg gcatcttcgt caccggcaat gggctttccg gtttcccaag cttgttctag
     6001 gtattcgccg ggtttacctc gcagatggct tcgggccaac taacccgacc gacgctttct
     6061 ccaagatcgc tcaggttttc cacactttcg acggccgcct ttgcgcggcc aggccggctg
     6121 tctggacagt cgtccgaata acagggatag ttgtccacat cccatccggt gtggtccagt
     6181 cgcggaaacc atttggtgaa agcccgcgtt ttcgacgcgc gactgcccta agattcagtt
     6241 gtggccgtag gcacgcgtct ggtcacctcg ggtggatgct gggctttgtg ttgtcggagc
     6301 cgttgacgcg gctggatgtg ttggggttgt gatggttcct gcttttacgt tcttgccgcc
     6361 ggagatcaac tcggccctga tgtactcggg cgccggatcg gggccgttgt tcacggcggc
     6421 gtcggcctgg gacgggttgg cgtcagacct gtcgggcgcc gcgtcgtcgt ttcaatcggt
     6481 gatcaacggg ttggccggtg gaccatggtc gggtccggcc tccgcgtcga tggtcgcggc
     6541 ggcgacgccg tacgtggggt ggttgagcgc ggctgccatt caggccgagg cagcggccgg
     6601 tcaggcccgg gcggcggcga cggcgtttga gtccgcgttg gcgggaacgg taccgacgtc
     6661 cgcggtgacg gcgaatcgcg tgcagctgat gacattgatc gccacgaaca tcctcggtca
     6721 aaacacgccg gcgatcgcga tgaccgagtt cgaatacatg gagatgtggg ctcaggacgt
     6781 ggccgcgatg gtgggctatc acgtcggggc gaccggggtg gcggcggcgt tgccgtcgtt
     6841 cagcgtgccg ccggcaggtc tggcgggcct gggcgggttg ttggcgtcgc cgctggtcgg
     6901 tgtggcgtcg gaggtgtccg gggtggcatc gtcggcgggc ggcgtgttgg cctcgggtgt
     6961 cacgtcggcg gtcgggcctg cggtggcggg ggtgtcgacg gtggcttccg cgttgccggt
     7021 gtcggaaatt tcacaggtgg cccagatcgc gatgtatccg gccagcatga tgatgtcgcc
     7081 gatcatgatg gcggcccagg ctggaatggg tgcgcatccg gcgctggccg gtgcggatat
     7141 ggccgccgcc ggcccgaagt tcgtcagtga cgccgttccg aagccgctgg gtggcgcggg
     7201 cctgggcggc ctgggcggtg ctgcgggagg cttgggtaag gcacgtctgg tcggggcgat
     7261 gtcggttccc ccgacgtggc agggcgcggc accgtctcgg atggtcagct cggcgatgtc
     7321 ggggctgggt agcgagctgg ccggcgctgc cgcaccggcc ggcgcaccga tgggcggtgg
     7381 catgccgatg atgccgatgc cgatgggtgg catgggcggc ggcggcatgc ccggtgggat
     7441 gttgggccgc ggcggcgcca gcccgaacca cgtggtccag tcgcggccta ccgtggtgcc
     7501 gcgaaccggc gtcggatagg cccgcgctgc gctaatccgg tccaccacga ccgtggcgcg
     7561 ttgcggtggt aatcgaaact tcggttggtt cgaccgccgg agaagcgtgg cgctgctgcg
     7621 gctgcaggcc gcggataacc ttgcagcccg tggtttttgg cgcgtcgcgt cggccgcacg
     7681 tggtcacccc gaacgggcgc accgcagccc gacgagtgga cgaaaaaact cggccccagt
     7741 cgactcccgc cgaccccagt gggccccaag tggaatggcc cgagcaaatc gggaatgacg
     7801 acagataaca cgggcgcgac acgccgaatc gcgtatcgag gctgatcgtc gccgtaacta
     7861 taccgtgatc ctcaaatgtg ggtgaggggc cttcacggaa gggcgcgcgg tgagcagaaa
     7921 ccccgtcgac ggaatgtccc gtcgagaatt tttctccaaa gtcgccgccg ccaccagcgc
     7981 cggggccgtg atgtcgctgg ccgctccggt gatcgaaaag gcttatgggg caggcccatg
     8041 ttcgggccat ttgaccgaca ttgaacattt cgtgttgtta atgcaggaga accgttcctt
     8101 cgatcactac ttcggaacgc tctccggcac cgacggattc gacacggcgt ccgcggcttt
     8161 tcagcaaaag ggttggaacc cgatgacgca gacgctcgac cccaacggcg tcacgatccc
     8221 ctaccgtttc gacaccaccc ggggccccct gcaggacggc gaatgcgtca acgaccccgg
     8281 ccacgactgg atcaccatgc acggggcctg gaacaacggc aacaataacg gctggttgcc
     8341 ggcgcaggcc aacgagagtc ctttggtggg caatgtcccc gtgacaatgg gctattacac
     8401 gcgccgcgac ctgcccgtcc attacctact ggccgacact tttacgatct gcgaccaata
     8461 ccactgttcg ctgttgggcg ggaccacgcc caacaggctg tattggatga gcgccaccat
     8521 cgaccccgac gggacgcacg gcggaccgca actcgtcgag cccaacgttc aaccaataca
     8581 gcggttcagc tggaccatca tgccggagaa cctcagcgcc gccggggtta gctggaaggt
     8641 gtatcagaac aagctgcttg gggctctcaa caacaccgtc atcggctaca acgggctggt
     8701 caacgacttc aagcagtcgg caaatccgca gtcggacctg gctcgcaacg ggattgctcc
     8761 gacctacccc gggaactttg tctccgatgt caggaacaac acgctgccgg cggtttcctg
     8821 gatacttccg gggttcctct actccgagca tccctcgttt ccggtgtgcg tcggcgcggt
     8881 cgcgatcgtt gacgtgctgc gcacgttgct atccaatccc gcggtgtggg aaaaaaccgc
     8941 actgatcgtc acctatgacg aaaacggcgg cttcttcgat cacgtcacgc caccaacacc
     9001 accgcccggt acgccgggtg aattcgtcac ggtgcccgat atcgacgcgg tgcccgggtc
     9061 cggtggcata cgggggccaa tcggtctggg ctttcgcgtg cccactttcg tcatttctcc
     9121 gtacagccgt gggccgctca agtgcagcac cgttttggac cacacatcac aattgaaatt
     9181 gctgcggacg agattcggcg tgccggttcc taacctgagt ccgtggcgcg atcagacggt
     9241 cggcgacatg acgaccgcgt tcaacttcgc cgtcccgccc aacccgtcga gacccagttt
     9301 ggaccacccg gtgctggcgg cgctcccgaa actgccacag tgtgtcccca atgcggtgct
     9361 cggatcgacc gtgaagacat cgattcccta tcgggtgccg tacccgcagt cgatgccgac
     9421 ccaggaaacc gcgccgcttc gcggaactcc cagcggaatc tgctgaattg cgctgaattg
     9481 gattgacagc ggccggatgc gaccgccccg gaccttcgac tgccgcggat cgctcacggt
     9541 cattcgttgt cattccgcgg gcgccgaaat gtgcggcgcg aggcggattg ggcgagcgaa
     9601 ccgccggcag cccttcgctg caccccgtgg atggaatcgg gtgcctgccc ggcgtcggcg
     9661 gttaggggcc tacacggtcg acaacgtgcg ttttcatccg gtccacccgt tggtccaacg
     9721 gcaagggcga cgggttttgc tgcatcgggg tggtcctgac atccggcggc ggcgcgagtg
     9781 gggacggtgg gggcccggag gctccgcgga ccggtgatgc gtgtcggccg gtgcgaggtt
     9841 ttcgggacca cgacttgggc gaaaagcgct gcatgacaac gacttcagct ctttgccggc
     9901 gcgccgtggg gcggatctga aagcgccgtc gaatggccgg tctcgcgggc ctgcagggcc
     9961 gggttcccgg ttgctgcgac ccgtttcgtt cccaggattg cgtgtgctca aaggggagcg
    10021 aaatcggcaa attcaggata ttgtctgtgg gattgactta cgatgcggag ggctcggtgg
    10081 ttgattcgtt ggccaccgcg agtgagtgta cgtagtagat cggctgtgga agggggcagc
    10141 gtgcttttgc ctcttggttc gccgctgccg gacgattcgg tggcagcggt tcggggtacg
    10201 tcgggcatgc tcgggggcct atcggttccg ctcaaatggg gcgtggtcgt cccaccggac
    10261 aactatgacc attgggcgcc agagcccgaa gagggcgtca gtgccggtga cgatgtggcc
    10321 gacgggctgg ccccggaggc taccgctgag ggcatggacg agtggaacga gtggggcgaa
    10381 tggagcgggt gggaaggcga agccgaacct cgcttcgacg tgccgcggac tagcagcgtg
    10441 atcccacatt cgccggcggc cggttgagga gagggggacg actgtcggtg tttcactcat
    10501 gatcggcggc tccgatatcc ccgtggccgg ccggtaacaa agaccaatct gtatggcaag
    10561 tccagaaaat taaccagaag gagaaaggca acatggcacg ttttatgact gacccgcacg
    10621 agatgcgggc gatggctggt cgttttgaga tgcacgcgca gacggttgag gatgaggccc
    10681 gcaagatgtg ggcgtcgtcg atgaacattg cgggtgcggg gtggagtggt caggccc
//
LOCUS       DASSGQ010000125         4523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1284875,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000125 DASSGQ010000000
VERSION     DASSGQ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284875"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..844)
                     /locus_tag="VFI55_05845"
     CDS             complement(98..844)
                     /locus_tag="VFI55_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7073590.1"
                     /translation="MGFDVPPDAYVRFMGRYAEPLAEVFAAFAGVGSGDRVLDVGCGP
                     GALTARLLTIGAEVAAIDPSPPFIDGIRVRFPDVDVRRGTAEELPYDTATFDASLAQL
                     VVHFMTDPLVGVRQMARVTRRGGVIAACVWDGPTGALAPFWDAVHLIDPEAEDEALLF
                     GARMGHLTELFEAAGLRDVEENAIAVDVAHPTFEEWWEPYTYGVGPAGDYVQRLDGDG
                     RALLESLARERMGSGPFTVTATAWAARGTV"
     gene            complement(895..1968)
                     /gene="ychF"
                     /locus_tag="VFI55_05850"
     CDS             complement(895..1968)
                     /gene="ychF"
                     /locus_tag="VFI55_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET7073591.1"
                     /translation="MSLSLGIVGLPNVGKSTLFNALTRNNVVAANYPFATIEPNEGVV
                     SLPDPRLDKLAQLFHSERIVAAPVTFVDIAGLVKGASEGAGLGNKFLAHIRECDAICQ
                     VVRVFSDDDVTHVAGQVDPKSDIEVVETELILADLQTLERALPRLEKEARTNKERRPV
                     YEAAQAAQATLDGGTTLFAAGADASLLRELNLLTTKPFLYVFNADEAVLTDAARVAEL
                     RRLVAPADAVFLDAAIEAELAELDDDSAAELLESIGQTERGLDALARAGFHTLKLQTY
                     LTAGPKEARAWTIHQGDTAPKAAGVIHTDFEKGFIKAEIVSYDDLIAAGSMAAAKAAG
                     KVRMEGKDYVMADGDVVEFRFNV"
     gene            2100..3329
                     /locus_tag="VFI55_05855"
     CDS             2100..3329
                     /locus_tag="VFI55_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6542 domain-containing protein"
                     /protein_id="HET7073592.1"
                     /translation="MSAQRARSAVEAGHRSIHPNLPGVPWWGALLIATIATAVGYAID
                     AASDHKELTHVFAGFYIAGCVAAVLAVRQEGVFTAIIQPPLILFCAVPGAYWLFHGGK
                     VGGVKDLLINCGYPLIERFPLMLGTAGAVLLIGMVRWYFGKSRGTAVAADTTDDAASA
                     SPARSNSIFNAFLDAVAPKLNSLLRLHSRADDADDAASGDATGTRDPSAPRKAKSGRP
                     ARRTGSRPGRAHSRHARPLGDEDERVAERPRRRRQAPPPDFDPADPPRRSRRRPRPQG
                     DPELRGRRESRPDSRQARANPYERPYEGSYEGTRPRSSRFEGYDRYDPPPAPEQFNRY
                     EPYEPRRRRAPGGNGANPTHHPISQVRYRGSGPLDEPRAPRGAEPPADPRPERRNRSR
                     SGRSEGRSPAESWEYEG"
     gene            complement(3366..4373)
                     /locus_tag="VFI55_05860"
     CDS             complement(3366..4373)
                     /locus_tag="VFI55_05860"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET7073593.1"
                     /translation="MPPTVDMGIPGTVRSGEPAAGAAARKRVLLAEPRGYCAGVDRAI
                     ETVERALEKHGAPVYVRHEIVHNRHVVSTLEKAGAVFVEETDAVPEGAIVVFSAHGVA
                     PTVYAAAAARSLHTIDATCPLVTKVHNEARRFARDDYDILLIGHEGHEEVIGTAGEAP
                     EHVQLVDGVASVDNVVVRDENKVVWLSQTTLSVDETMEIVERLRQRFPKLQDPPSDDI
                     CYATQNRQVAVKAMAPECELVIVVGSRNSSNSVRLVEVALGGGAAAAHLVDWADDIDP
                     AWLEGVTTVGVTSGASVPEVLVQGVLARLSDCGYDVVQPVTTAQETLVFALPREIRSP
                     R"
BASE COUNT          761 a         1586 c         1452 g          724 t
ORIGIN      
        1 ccacctttca agaatttccc tcagaccgga atccaggcaa gcagtgattc ccattcggct
       61 gactagacgt ttgaagcgga actcgaccac ttcgtgatca aaccgttccg cgggcggccc
      121 aggcggtagc ggtgacggtg aacgggccac tacccatacg ttcgcgagcc agtgattcca
      181 gaagtgcgcg accatcgcca tcgagtcgct ggacatagtc gccagcagga ccgacaccgt
      241 aggtgtacgg ctcccaccac tcctcaaagg tcggatgcgc aacatcgacc gcaatggcat
      301 tctcttcgac atcgcgcagg ccagcagctt cgaacagctc agtcaggtgc cccatgcggg
      361 ccccgaagag tagcgcctca tcctcagctt cggggtcgat caggtgaacc gcatcccaga
      421 acggcgccag cgcgccggtc ggcccgtccc acacgcatgc cgcgatgaca ccgccacgcc
      481 gcgtcacgcg cgccatctgt ctgacgccga ccaatggatc cgtcatgaag tgcactacca
      541 attgcgcgag agaagcatcg aaagtagcag tgtcgtacgg caattcctcc gctgtacctc
      601 gacgcacatc aacatctggg aagcgcacac ggatcccgtc gatgaacggt ggcgaaggat
      661 cgatggcagc gacctcggct ccgatcgtca acaaacgcgc cgtcagcgcg cccggaccac
      721 agcccacgtc gagcaccctg tcgcccgagc cgaccccggc aaatgccgcg aagacctccg
      781 ccagaggctc ggcgtaccgg cccataaaac ggacataggc gtctggcggg acgtcaaaac
      841 ccaccagtga atgctacggc tgtcaacggc gtcggctact gcttcttggg ttgactagac
      901 gttgaagcgg aactcgacca cgtcgccgtc ggccatcaca taatctttgc cctccatgcg
      961 gaccttgccc gccgccttgg ccgccgccat cgacccggcc gcgatcaggt cgtcgtagga
     1021 cacgatctcg gccttgatga agcccttctc gaagtcggtg tggatcaccc cggcggcctt
     1081 gggcgcggta tcgccttggt ggatggtcca cgcccgcgcc tccttcgggc ccgccgtcag
     1141 gtaggtctgc agcttcagcg tgtgaaatcc cgctcgggcc agcgcatcga ggccgcgttc
     1201 ggtttgcccg atcgactcga gcagctcggc ggccgagtcg tcgtcgagtt cggccagctc
     1261 ggcttcgatg gcggcgtcca ggaacaccgc atccgcgggc gcgacgagcc ggcgcagctc
     1321 ggcgacccgc gcggcgtcgg tcagcaccgc ctcgtcggcg ttgaagacat acaagaaagg
     1381 tttggtggtc agcagattca gctcgcgcag cagcgaggcg tcagcccccg cggcgaacag
     1441 cgtggtgccg ccgtccaacg tcgcctgagc ggcctgcgcc gcctcgtaca ccggtctgcg
     1501 ctccttgttg gtgcgagctt ccttctccag gcgcggcagc gcgcgctcca gggtctgcag
     1561 gtcggccagg atcaactcgg tctcgacgac ctcgatgtcg gacttgggat ccacctggcc
     1621 tgccacgtgc gtcacgtcgt cgtcggaaaa cacccggacc acctggcaga tggcgtcgca
     1681 ttcgcggatg tgggccagga acttgttgcc cagcccggcc ccctcggagg cacctttgac
     1741 caggccggcg atgtcgacga aggtgaccgg ggccgccacg atgcgctcgg aatgaaaaag
     1801 ctgcgccaac ttgtccagtc gcggatccgg tagcgaaacg acaccctcgt tgggttcgat
     1861 cgtcgcgaac ggataattgg ccgccacgac gttgtttcgg gtcagcgcgt tgaacagtgt
     1921 cgacttgccg acgttgggca gacccacgat tcccaggctc aggctcacgg ggaaccaagt
     1981 ctagggggca tcggtgaggc cggccgtcac ggaccatccc cgcgcgacgg catggcacgc
     2041 cagggctgcg actggctata tatacggggc tttcggctca tagccggtac ggtctagatg
     2101 tgtcagcaca gcgggcaagg tcggcggtgg aggctggtca ccgctcgatt cacccaaatc
     2161 tcccaggcgt gccgtggtgg ggtgcgctgc tcatcgccac gatcgcgact gccgtcggct
     2221 acgcgatcga cgcggcgtcc gaccacaagg agctgaccca cgtcttcgcg ggcttctata
     2281 tcgccggctg cgtggccgcg gtgctggccg tgcgccagga aggtgtgttc accgcgatca
     2341 tccagccgcc gttgatactt ttctgcgcgg tcccgggcgc ctactggctg ttccacgggg
     2401 gcaaggtcgg cggcgtcaaa gacctcctga tcaactgcgg ctatccgctc atcgagcgtt
     2461 tcccgctgat gctgggcacc gccggcgccg tgctgctgat cggaatggtc aggtggtatt
     2521 tcgggaaatc acgtgggacg gcggtggcag ccgacaccac tgacgacgcc gccagcgcgt
     2581 cccccgcaag gtcgaactcg atcttcaacg ccttcttaga cgccgttgcc ccgaaactga
     2641 attcgcttct tcgcctccat tcccgcgccg acgacgccga cgacgcagcc tccggcgacg
     2701 cgacggggac ccgcgatccg agcgctccgc ggaaggcgaa gtccgggcgg ccggcgcgca
     2761 ggaccggctc acgtccgggc cgggcccact cccggcatgc ccgaccgctg ggagacgagg
     2821 acgaacgcgt ggccgaacgt ccgcggcggc ggcgccaggc tcccccgccg gacttcgacc
     2881 cggccgaccc gccccgccgg tcgcgccgtc gcccgaggcc gcagggtgat ccggagttgc
     2941 gcggccgccg tgaatcccgt cccgattcgc ggcaggcccg cgccaacccg tatgagcggc
     3001 cgtacgaggg gagttacgag ggaacacggc ctcgtagcag ccgcttcgag gggtacgaca
     3061 ggtacgaccc cccgccggcc cccgagcagt tcaaccggta cgaaccgtac gagcctcggc
     3121 gccgccgtgc cccgggcggc aacggcgcca acccgacaca ccacccgatc tcgcaggtcc
     3181 gctaccgcgg gtccggcccg ctggacgagc ccagagcgcc gcgcggcgcc gagcccccag
     3241 ccgatccacg ccccgagcgc cggaatcgat cgcggtccgg ccgctccgag gggcgatccc
     3301 cggcggaatc ctgggagtac gaaggctagt ccgaggctga gtccgaagat cggaccggcc
     3361 ggctgctagc gaggcgaccg gatctcgcgg ggcaacgcga acaccagcgt ctcctgggcc
     3421 gtcgtcacgg gttgcaccac gtcgtagcca cagtcggaga gccgcgcgag cacgccctgc
     3481 accagcacct cggggacgga cgcaccggag gtgaccccta ccgtcgtgac cccctccaac
     3541 cacgccgggt cgatatcgtc ggcccagtcg accaggtgag cggccgctgc gccgccgccc
     3601 agcgccactt cgacaagccg caccgaattc gacgagttgc gggacccgac gacgatgacc
     3661 agctcgcatt cgggggccat cgccttgacc gcgacctgcc ggttctgggt cgcgtagcaa
     3721 atgtcgtcgc tgggcggatc ctgcagcttg ggaaaacgct gccgcagccg ctcgacgatc
     3781 tccatcgtct cgtcgaccga cagcgtggtc tgggacagcc agaccacctt gttctcgtcg
     3841 cgcaccacca cgttgtccac cgaggcgacc ccgtcgacca gctgcacgtg ttcgggcgcc
     3901 tccccggcgg tcccgatgac ctcttcgtgg ccctcgtgac cgatcaacag gatgtcgtag
     3961 tcgtcccggg cgaatcgcct ggcttcgttg tgcaccttgg tcaccaacgg gcaggtggcg
     4021 tcgatggtgt gcaggctgcg tgcggcggcg gcggcataca ccgtcggtgc gacgccgtgc
     4081 gcggagaaca ccacgatggc tccctcggga accgcgtcgg tttcctcgac gaaaaccgcc
     4141 cccgcctttt cgagggtgct gaccacgtgg cggttgtgca cgatctcgtg tcggacgtag
     4201 accggcgcgc cgtgtttctc cagcgcgcgt tcgacggtct cgatggcgcg atccacaccc
     4261 gcgcagtagc cacgcggctc ggcaaggagc acccgcttgc gggcggcagc gccggccgcg
     4321 ggttcccccg acctcacggt gccgggaatt cccatgtcaa cagtcggcgg catgacgttc
     4381 agggtacgtt ctggcgcctt ggagagcaca tctgccgccg gctcgcgccg ctgggcttgg
     4441 aagttagccg cccgttaagg cactcttgga cccatggcaa ctgcaccgta tggagttcgg
     4501 ctgctggtcg gcgcggcgac agt
//
LOCUS       DASSGQ010000126        11069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1133324,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000126 DASSGQ010000000
VERSION     DASSGQ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11069
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133324"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /gene="mshC"
                     /locus_tag="VFI55_05865"
     CDS             complement(<1..1232)
                     /gene="mshC"
                     /locus_tag="VFI55_05865"
                     /EC_number="6.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--1-D-myo-inosityl
                     2-amino-2-deoxy-alpha-D-glucopyranoside ligase"
                     /protein_id="HET7073594.1"
                     /translation="MQSWPTAQVPVLPGRGPELRLYDTSDRQVRPVAAGAQPGSKATM
                     YVCGITPYDATHLGHAATYLAFDLVHRQWLDLGHDVHYVQNVTDVDDPLLERADRDGV
                     DWRELGDREVALFREDMTALRILPPHEYVGATEAIPEVVELVEKMLASGAAYVLDPEI
                     GEYPDVYYRADATLQFGYESGYDRDTMLRLFAERGGDPDRRGKTDELDALLWRAARPG
                     EPSWPSPFGAGRPGWHIECAAIALSRIGSGLDIQGGGSDLIFPHHEFTAAHAECVRGE
                     RRFARHYVHSGMIGWDGHKMSKSRGNLVLVSTLRAQGVEPAAIRLGLLAGHYRADRFW
                     SDEVLDEAVARLQRWRTATALPAGPDATDLIARVRQYLADDLDSPKAIAALDGWTTDA
                     LEYGGHDDGAPRLVATAV"
     gene            complement(1385..2161)
                     /locus_tag="VFI55_05870"
     CDS             complement(1385..2161)
                     /locus_tag="VFI55_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET7073595.1"
                     /translation="MSDLTDAALAADLAAEAGELLLKVRKDIGFGQPWALGDAGDSQA
                     NDFILRRLRVERPDDAVLSEEAYDDLVRVRCDRVWIIDPLDGTREFSTPGRDDWAVHI
                     ALWQRPVDGRREITDAAVALPARGNTVYRSDTVTDGDARVGIPHTFRIAVSATRPPAV
                     LHRMRQKVAIQPVAIGSAGAKAMALIDGDVDAYVHAGGQWEWDSAAPAGVVMAAGMHA
                     SRLDGSPLRYNQPDPYLPDFIMCRAELAPILLDAIHQVWR"
     gene            complement(2194..3018)
                     /locus_tag="VFI55_05875"
     CDS             complement(2194..3018)
                     /locus_tag="VFI55_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HET7073596.1"
                     /translation="MTPRNNDCDEREVLRDGELTVLGRIRSASNATFLCESTLGGASV
                     HCVYKPVSGEAPLWDFPDGTLAGRELGAYLVSTQLGWNIVPYTVIRDGPAGLGMLQLW
                     VRQPGDAADSDPRPGPDLVDLFPADKLPPGYLPVLRAYDYAGDEVILMHADDTQLRRM
                     AVFDVLINNADRKGGHILFGLDGRVYGVDHGVCLHVENKLRTVLWGWAGKPVDDQTLE
                     AVAGLAAALSCSLGDELATHITRAEIAALRRRARALLNDPVMPGPNRHRAIPWPAF"
     gene            complement(3002..3589)
                     /locus_tag="VFI55_05880"
     CDS             complement(3002..3589)
                     /locus_tag="VFI55_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HET7073597.1"
                     /translation="MARAIHVFRTPDRFVAGTVGQPGNRTFYIQAAHDSRVVSVILEK
                     QQVAVLAERIGALLIEVNRRFGTPVPPEPAEIDDLNPLITPVDAEFRVGTMGLGWDSE
                     AQTVVVELLAVTDAEFDASVVLDDTEDGPDAVRVFLTPESARQFATRSNRVISAGRPP
                     CPLCDEPLDPEGHICARANGYKRGALLGPDDDPPE"
     gene            complement(3655..4410)
                     /locus_tag="VFI55_05885"
     CDS             complement(3655..4410)
                     /locus_tag="VFI55_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7073598.1"
                     /translation="MTVILLRHGRSTSNTAGILAGRSEGVDLDDRGREQAEGLIDRIG
                     DLPIRAVVTSPLLRCRRTVAPLAESLCVEPLIDDRLAEVDYGDWTGRKIGELANEPLW
                     RVVQAHPSAAVFPGGEGLAQVQARAVAVVREHDRRLASEHGGDALWVACTHGDVIKAV
                     VADAYGMHLDGFQRVTADPGSVSVIRYTPLRPFVLHVNHTGARLSAMLRAGPPPKAES
                     EGDEPHGESKAAPQTESTAVVPSSDAVVGGSTN"
     gene            complement(4536..4838)
                     /locus_tag="VFI55_05890"
     CDS             complement(4536..4838)
                     /locus_tag="VFI55_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073599.1"
                     /translation="MTVIADRHIAAREFAVEDISTGVFASGYGQVGDGRSYSFHIEHR
                     SLVVEIYRPRLAGPVPQADEVVARAVRSLVDIDLTDERSLTAAVRDAVAHALPVSR"
     gene            4951..6039
                     /locus_tag="VFI55_05895"
     CDS             4951..6039
                     /locus_tag="VFI55_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073600.1"
                     /translation="MRQHRGDKHRVRRRLAALLLLLASVTSCSSNPLGTFASEKPRTI
                     EPAQAAQSPPVSQNPAGTVRALAGEATAALFDGSTRQLAVLVPGADPAAPASITEFDD
                     PHATPRGSPRQVALPGPATALTGDGRGWAFLAARGGYFAVDLSTGHTAHVPVADAAQA
                     EFTAIAQRADGKLVLGSADGAVFTLTSQTPAAVSTASVDSRNKIFARVDALATQGNTT
                     VVLDRGQTSVTTIGADGRVEQSLRAGEGATTLAADALGRVLVADTRGGQLLVYGVDPL
                     ILRQAYPVRQAPYGLAGSRELTWVSQTASNTVIGYDLSTGIPVERARHPTVRQPNSLA
                     FDEASDTLYVVSGSGAGVQIIEHAAGTP"
     gene            6093..6299
                     /locus_tag="VFI55_05900"
     CDS             6093..6299
                     /locus_tag="VFI55_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 family protein"
                     /protein_id="HET7073601.1"
                     /translation="MSDEYEWVPLRLPPELTRLSASTRLSIEAEYRGWELTRVRLYTD
                     GSRRVLLRRKKSRLDSQRPDQPQL"
     gene            6296..7423
                     /locus_tag="VFI55_05905"
     CDS             6296..7423
                     /locus_tag="VFI55_05905"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HET7073602.1"
                     /translation="MTAAPPGRGGHRLYGLVRPLLFVVPPERIHTLVFAVLRGLTAGA
                     SVRRLLHRLLGPTDPVLASTVFGVRFPGPLGLAAGFDKNGTGLLTWGACGFGYAEVGT
                     VTAHPQPGNPAPRLFRLPADRGLLNRMGFNNRGAGALALQLARHRPEIPIGVNIGKTK
                     TTPSADAVNDYRASARLVGPLASYLVVNVSSPNTPGLRDLQAVESLRPILEGVLLEAS
                     ATSTPVLVKIAPDLADSDLDEIADLAVELGLAGIVATNTTVSRDGLLTPGVDALGAGG
                     ISGPPVARRAVEVLRRLHGRAGDRLALISVGGIETGDDAWERITAGASLLQGYTGFIY
                     GGGLWPKHIHDGIARRLHDGGFASLSDAVGSAAPRSGKSPG"
     gene            complement(7469..8077)
                     /locus_tag="VFI55_05910"
     CDS             complement(7469..8077)
                     /locus_tag="VFI55_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073603.1"
                     /translation="MRTPNAVLPQRIYLIRHGEKPADPPASSPDQSGPPFGIDADGNQ
                     NVHSLLPRGWQRAGALAVLFDPAVGAPLAGLHTPAALYSPNYGKPEKTQAHRTFQTIQ
                     GLSGRLGITIQSPVPEGEEPALADAVLASGSEVVLICWEHQHIPAFAQEVPTGNNTAV
                     PTQWPGDRFDVIWTFDLDPATGRYVFGQVPQQLLEGDTATVI"
     gene            complement(8083..8217)
                     /locus_tag="VFI55_05915"
     CDS             complement(8083..8217)
                     /locus_tag="VFI55_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073604.1"
                     /translation="MVTGPVGVQHIVVPHVSAHADLFDGQRAHLHLIRAELEACQTPR
                     "
     gene            complement(8428..8703)
                     /locus_tag="VFI55_05920"
     CDS             complement(8428..8703)
                     /locus_tag="VFI55_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073605.1"
                     /translation="MAPTITGTADFVTGEGFLPGRRITICVTYIEDDVSDYLSYTAGP
                     DGRLHARVPTSHAPGALRIRATDHRSDPDGECGLLWSNAHTASPPKH"
     gene            complement(8960..9640)
                     /locus_tag="VFI55_05925"
     CDS             complement(8960..9640)
                     /locus_tag="VFI55_05925"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7073606.1"
                     /translation="MLDGAFAVLEALAAADGLGLTELACASGLPKSSAYRLAEQLAAL
                     GAVQRVQRRYYVGARIGHIGQRWQPDPQLRDAGQAPVHTLAVQSRATASLRILHEDRL
                     RAICTTAPHGHAYLPNPADPESTARTATGRVLYAAQANTDGVALPGCWTRREWRLLRA
                     RIRDAHATVVDHQEAVAGICCVSAPVWWPNGSCAGAVTAVVQAARPPHDLRDQVIFAA
                     RSISARLP"
     gene            complement(9889..10467)
                     /locus_tag="VFI55_05930"
     CDS             complement(9889..10467)
                     /locus_tag="VFI55_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HET7073607.1"
                     /translation="MNRLDLVVGPNGAGKSTFVELTLAPLLPRSGYVNADEIARQRWP
                     DDPAAHAYDAARVAADTRARLIDLRRSFIAETVFSHPSKLELIDTAQTAGYTVVLHVL
                     LIPEELAVQRVRHRVEAGGHDVPEDKIRQRYQRLWALVGTAIIRCDTATVYDNSALKG
                     PRIVAHMSGGQPIGAPAWPAWTPQELVARCPA"
     gene            complement(10481..10891)
                     /locus_tag="VFI55_05935"
     CDS             complement(10481..10891)
                     /locus_tag="VFI55_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073608.1"
                     /translation="MANNADRVTRFAVDLLDSAAVEGARQSRSAKQQLDHWARVGRAV
                     SSQHTAARRRVEAALAGDLELRELSVEEGVVFNAEIAAALQENLAESNYGQLLAQRGI
                     TTVAQNEAGEIVEHRPDGTSIVLTAKRRPARSRK"
BASE COUNT         1730 a         3764 c         3791 g         1784 t
ORIGIN      
        1 tcaaccgcag tcgccaccag cctcggcgcc ccgtcgtcat ggccaccgta ttccagtgcg
       61 tcggtggtcc aaccatcaag tgccgcaatg gctttggggg aatcaagatc atcggccaaa
      121 tactgccgca cccgggcgat caggtcggtg gcgtcgggac cggcgggcag cgccgtcgcg
      181 gtgcgccagc gctgtagccg ggcgaccgcc tcgtcgagca cttcgtcgct ccagaaccgg
      241 tcggcgcgat agtggccggc cagcaggccc agccggatgg ccgcgggctc aacgccttga
      301 gcgcgcaacg ttgagaccag caccagattg ccgcggctct tggacatctt gtgcccatcc
      361 cagccgatca tcccgctatg cacgtagtgc cgggcgaatc gccgctcgcc ccgcacacat
      421 tcggcgtgcg cggcggtgaa ttcgtgatgg ggaaagatca ggtcgctgcc gccgccctgg
      481 atgtccaggc cgctgccaat gcggctgagc gcgatcgccg cgcattcgat gtgccagccg
      541 ggtcgcccgg caccgaacgg tgagggccaa ctgggctccc ccggtcgcgc ggcccgccac
      601 agcagggcgt cgagctcgtc ggtcttgccg cgccgatccg ggtcgccacc gcgctcggcg
      661 aacagccgaa gcatggtgtc ccggtcgtag ccggactcgt agccgaactg aagtgtggcg
      721 tcggcccggt agtagacgtc cgggtattcg cctatttccg ggtcgagcac gtaggcagcc
      781 cccgaagcca gcatcttctc gacgagttcg accacttcgg gaatcgcctc ggtcgccccg
      841 acgtactcgt gtggcggcag tatccgtagc gccgtcatgt cctcgcggaa aagtgcgacc
      901 tcccggtcgc cgagctcccg ccagtcgaca ccatcgcgat cggcgcgctc caacaaggga
      961 tcgtcgacgt cggtgacgtt ctgcacgtag tggacgtcgt gaccgaggtc gagccactga
     1021 cggtgcacca ggtcgaacgc cagataagtg gccgcgtggc ccagatgggt tgcgtcgtac
     1081 ggcgtgatcc cgcagacgta catggtcgcc ttggaacctg gctgagcgcc ggccgccacc
     1141 ggtcgcacct gccggtcaga ggtgtcgtaa agccgcagtt cggggccccg tccaggcaac
     1201 accggaacct gcgcggtagg ccacgactgc atgcctcgac tttaagcccg gcgacgatgc
     1261 ggcccgcgta gcgggctgag aaggagccgg gcaatccagc acaagcccgg cgacgatgcg
     1321 gcccgtgttg ggggaaccgc ccagcggcgt agccgcgagg gggacgggct gaggcggggg
     1381 ctcctcagcg ccacacctgg tggatcgcgt cgagcaggat cggcgccagc tcggcgcgac
     1441 acatgatgaa gtcgggcaga tatgggtcgg gctggttgta gcgcagcgga gagccgtcga
     1501 gcctggatgc gtgcatgccc gcggccatca ccaccccggc cggcgccgcc gaatcccatt
     1561 cccactggcc accggcgtgc acgtaggcgt cgacgtcccc gtcgatgaga gccatcgcct
     1621 tggcgcccgc cgacccgatc gccacgggct ggatggccac cttctgccgc atgcggtgca
     1681 acacggccgg tggccgggtg gcgctgacgg cgatccggaa ggtatgggga atgccgacgc
     1741 gcgcgtcgcc gtcggtcacc gtgtcgctgc ggtagaccgt gttgccgcga gcgggcaaag
     1801 ccactgccgc atcggtgatc tcgcgccggc catcgacggg acgctgccac agcgcgatgt
     1861 gcaccgccca gtcgtcgcgc cccggcgtgg agaactcccg ggtgccgtcc agcgggtcga
     1921 tgatccacac ccggtcgcac ctgacccgga ccagatcgtc gtacgcttcc tcgctgagaa
     1981 ccgcgtcgtc gggtcgctcg acgcgcaacc tccgcaaaat aaaatcgttt gcctgactgt
     2041 cacccgcgtc gccgagtgcc caaggttggc cgaaaccgat gtccttgcgc accttgagca
     2101 acagctcccc cgcctccgcg gcgagatcgg cggccagagc ggcatccgtc aggtctgaca
     2161 ccggataagt attgccgacg ccgggggcgc gtactaaaac gccggccacg ggatcgcgcg
     2221 atgccggttc ggtccgggca tgaccggatc gttgagcagc gcgcgggccc gccggcgcag
     2281 tgccgcgatt tccgcccggg tgatgtgcgt ggccagttca tcgccgagag agcacgagag
     2341 ggctgcggcg aggcccgcca ccgcctccag agtctggtcg tcgaccggct taccggccca
     2401 gccccataac accgtgcgca gtttgttctc cacgtgcagg cacacaccgt ggtcgactcc
     2461 gtaaacccgg ccgtcgagcc cgaacaggat gtggccgccc ttgcggtcgg cgttgttgat
     2521 cagcacatcg aacaccgcca tccgccgcaa ttgggtgtcg tcggcgtgca tcaggatgac
     2581 ctcgtcgccg gcatagtcgt acgcgcgcag caccggcaga taaccgggcg gcagcttgtc
     2641 ggcggggaag aggtcgacga ggtcgggacc tggcctgggg tcggaatcgg cggcgtcacc
     2701 gggttggcgc acccaaagct gcagcatgcc cagcccggcc ggaccgtcac gaatcaccgt
     2761 gtacggcacg atgttccaac ccaactgcgt cgacaccagg tatgcgccga gttcgcgacc
     2821 ggccagggtg ccgtcgggga aatcccacag cggtgcttca ccggagaccg gcttgtagac
     2881 gcagtgcacg gaggccccac caagcgtcga ctcgcacaaa aaggtggcgt tgctggctga
     2941 gcggatgcgt ccgaggacgg tcagctcgcc gtcccgcaac acctcgcgtt cgtcacagtc
     3001 gttattccgg ggggtcatcg tcaggcccga gcagcgcgcc gcgcttgtag ccgttggcgc
     3061 gtgcgcagat gtggccctcg gggtccaacg gttcgtcgca tagtgggcac ggcggacgtc
     3121 ccgcggagat cacacgattg gagcgagtgg cgaactgccg tgccgattcc ggcgtcaaaa
     3181 acacccgcac cgcgtccgga ccgtcctcgg tgtcgtcgag caccaccgaa gcgtcgaatt
     3241 cggcgtcggt cacggccaat aattcgacca ccacggtctg agcctccgaa tcccagccca
     3301 ggcccatggt cccgacccga aactccgcat cgacgggcgt gatcaacggg ttgaggtcgt
     3361 cgatctccgc gggctcgggc gggaccgggg tgccgaaccg gcgattgacc tcgatcagca
     3421 gcgcaccgat gcgctcggcg agcaccgcga cctgttgctt ctccaatatc accgacacca
     3481 cccgggaatc gtgcgccgct tggatgtaga aggtgcggtt tccgggctgg ccaacggtcc
     3541 cggccacgaa gcggtcgggt gtgcggaaga cgtgaattgc gcgggccatg gcacttccca
     3601 aaataccggc tgtgcccgtc gctgtgcgat ccgatcgtgg caccgcgacg gaatttaatt
     3661 ggtggagcct ccgaccaccg cgtcgctcga cggcaccacg gcggtcgatt cggtttgcgg
     3721 cgccgccttg gactcaccgt gaggctcatc accctcggac tccgccttcg gtggcggccc
     3781 ggcccgcagc atcgcggaca gccgcgcgcc ggtgtggttg acgtgcaaca cgaatggccg
     3841 cagcggggtg taccggatga cgctcaccga cccgggatcg gcggtgacgc gctggaaccc
     3901 gtccaggtgc atcccgtagg cgtcggcgac aaccgccttg atgacgtcac cgtgcgtgca
     3961 tgccacccag agcgcgtcgc cgccatgttc tgacgccagc cttcggtcgt gttcgcggac
     4021 tacggctacc gcgcgcgcct gcacttgcgc caagccctcg ccgccgggaa ataccgccgc
     4081 gctgggatgg gcctgcacga cccgccacag cggctcattc gccaactcgc cgatcttgcg
     4141 gccagtccag tccccgtaat cgacctcggc gagccggtcg tcgatcagcg gctcgacgca
     4201 cagcgactcg gccagcggcg cgacggtacg ccggcagcgc agcagcggcg aggtcaccac
     4261 cgcccggatc ggcaggtcgc cgatccgatc gatcagtccc tcggcctgct cgcgacccct
     4321 gtcgtcgagg tcgacgcctt cggaacgacc ggccagaatg cccgcggtgt tcgaggtgga
     4381 gcggccatgc cgcagcagga tgacggtcat gtcggcgcga ccgtcccgtt gcctggcagg
     4441 attagcacgg cgacaccggc aggaactcgg tcaaaccagg aaccacggcc acgacgacga
     4501 ccgtacccgg tgcgggtggc cgcgcccggg cttgactagc gggaaaccgg tagcgcgtga
     4561 gcgacggcgt cacgcaccgc ggcggtcagg ctgcgctcat cggtgaggtc gatgtcgacg
     4621 aggctgcgga ccgccctggc taccacttcg tcggcctgcg ggacggggcc ggccaggcgc
     4681 gggcggtaaa tctcgacgac gagcgaacgg tgctcaatgt ggaaggagta actgcgccca
     4741 tcaccgactt gtccatatcc gctggcaaag acccctgtcg atatgtcctc gacagcaaac
     4801 tctctagctg cgatatgccg gtcggcgatg acggtcatga agcgacgata cctctccagt
     4861 actactcagc gtaggaacat ggccgtattg gatgcgaaac cgttccttaa aattttgctt
     4921 tcacgatggt tacgcgaagg aaaataattg ctgcgacagc atagaggcga caaacatcga
     4981 gttcggcgac gccttgcagc tctgctgctt ttgctggcgt ccgtcacgag ttgttcatcg
     5041 aatccgctcg gtacgtttgc ctccgaaaaa ccgcgcacca tcgaaccggc gcaggcggcc
     5101 cagtcaccac cggtgtcgca aaatccggcc ggtacggtgc gagcgctggc cggggaagcc
     5161 acggcggcgt tgttcgacgg cagcactcgc caattggccg tcctggttcc cggtgccgac
     5221 ccggcggcgc ccgccagcat cacagagttc gatgacccgc acgcgacccc gcgcggctcg
     5281 ccccggcagg ttgccctgcc ggggccggcg acggcattga ccggcgacgg tcgtggctgg
     5341 gccttcctgg ctgcccgcgg cggctacttc gcggtcgacc tgtccaccgg ccacacggcg
     5401 cacgtacccg tcgccgacgc agcgcaggcc gaattcaccg cgatcgcgca acgcgccgac
     5461 ggcaagctgg tgctgggcag cgccgacggc gcggtattca ccctcacctc ccagacacca
     5521 gccgcggtca gcaccgcgtc cgtcgacagc cgaaacaaga tctttgctcg cgtcgatgcc
     5581 cttgcaacac aaggaaatac gacggtagta ctggacaggg gacagacctc ggtgacgacg
     5641 atcggtgccg acggtcgtgt tgagcagtcg ctgcgggcgg gcgagggcgc caccaccctg
     5701 gccgccgatg cgctgggccg ggtcctggtt gcggacaccc gcggcggcca actgctggtg
     5761 tacggcgtcg acccgctgat cctgcgtcag gcctacccgg tccggcaggc cccgtacggc
     5821 ctcgccggct cccgcgagct cacctgggtg tcccaaaccg catccaacac cgtcattggt
     5881 tacgatctgt caaccggaat tcccgtcgaa agggcgcgcc accccaccgt gcgacaaccc
     5941 aactccctgg cctttgacga agcgtcggac accttgtacg tggtgtctgg gtcgggtgcg
     6001 ggtgtgcaga tcatcgagca cgcggcgggt accccgtgaa cggccggcca gcgccgcggc
     6061 ggagcgggct tcccgcgggc tgggacaccg agatgtccga cgaatacgag tgggtgccgt
     6121 tgcgcctgcc gccggaactg acccgtctca gcgcatccac ccggctgtcc atcgaggccg
     6181 aatatcgcgg ctgggagctc acgcgggtgc gcctatatac cgacgggagt aggcgagttt
     6241 tgttgcgccg caagaaatcc cgcttggaca gccagcgacc cgaccaaccc caactgtgac
     6301 ggcggcccca ccgggccgag gcggtcatcg actttacggc ctggtgcgcc cgctgctgtt
     6361 cgtggtcccg cccgaacgca tccacacgct ggtcttcgcc gtgctgcgtg gcctcaccgc
     6421 cggagcgtcg gtgcgacggt tgctgcaccg cctgctcggc ccgaccgatc cggtgctggc
     6481 cagcaccgtg ttcggggtgc gcttcccggg accgctcggg ctggccgccg gcttcgacaa
     6541 gaacggcacg gggttactca cctggggcgc gtgtggattc gggtatgccg aggtcgggac
     6601 cgtcaccgcg cacccgcagc ccggcaaccc ggccccccgc ttgttccggc tgcccgccga
     6661 ccgcggcctg ctcaaccgga tggggttcaa caatcgcggt gccggcgcgc tggcgctgca
     6721 gctcgcgcgc caccgacccg agataccgat cggggtcaac atcggcaaga cgaagacgac
     6781 gccatccgcc gacgccgtca acgactaccg ggcaagcgcg cgcctggtcg gcccgctggc
     6841 gtcgtatctg gtggtgaacg tgagctcgcc gaacacgccg gggctgcgcg acctgcaggc
     6901 ggtcgaatcg ctgcggccca tcctcgaggg cgtcctgtta gaggcctcgg cgacgtcaac
     6961 gccggtactg gtgaagatcg cacccgatct cgccgactcc gatctcgacg aaatcgccga
     7021 cctggccgtc gagctgggcc tggccggcat cgtcgccacc aacaccacgg tgtcgcgcga
     7081 cggccttctc accccgggag tcgacgcgct gggcgccggg ggcatctcgg ggccgccggt
     7141 ggcgcgccgc gcggttgagg tgctgcgccg gctccacggc cgcgccggcg atcgcctggc
     7201 gctgatcagc gtcgggggca tcgaaaccgg cgacgacgcg tgggagcgga tcactgcggg
     7261 cgcgtcgctg ctgcagggct acaccggctt catctatggc gggggcctgt ggcccaagca
     7321 cattcacgac ggcattgccc gccggttgca cgacggcggg ttcgcctcgc tcagcgatgc
     7381 ggtcgggtcg gcggccccca gatcgggtaa gtcgccagga taggtcacgc tccgtctcct
     7441 accggcgcgc cgacgtacag cgcgtccctt agatcacggt ggcggtgtca ccttccagaa
     7501 gttgttgggg cacttggccg aacacgtagc gtccggtggc ggggtcgagg tcgaaggtcc
     7561 agatgacgtc gaatcgatcg cccggccact gggtggggac ggcggtgttg ttgcccgtgg
     7621 ggacttcctg tgcgaaggcc gggatgtgtt ggtgttccca gcagatcagg acgacctcgc
     7681 tgccgctggc cagaaccgcg tcggcgagcg cgggttcctc tccctcgggg accggggatt
     7741 ggatggtgat gccgaggcgc ccgctgaggc cctggatggt ctgaaaggtg cggtgcgcct
     7801 gcgtcttctc cggtttcccg taattcggtg agtacaacgc ggcgggcgtg tgcagccccg
     7861 ccagcggcgc cccgacggca gggtcgaaca gcacggccaa tgccccggcg cgctgccatc
     7921 cccgcggcag cagggagtgc acgttttggt tgccgtcggc atcaatcccg aacggcgggc
     7981 ccgactgatc gggcgacgaa gccggcgggt cggccggttt ctcgccgtgg cggatgaggt
     8041 aaatccgttg cggcagaacg gcgttgggtg tgcgcatggt cgtcacctcg gtgtctggca
     8101 cgcttcgagt tcggcgcgga ttagatgcag atgcgcccgc tggccgtcga acaagtctgc
     8161 gtgcgcggaa acatgtggca ccacgatgtg ctgcacaccg accggacccg tcaccatcgg
     8221 ccatcacctc ctgtgtcgtc acgcggatct caagccggtc gccgtgcgtg tgttgccggc
     8281 ggccgcgctt tggacgcgaa ctcgcgacat ccattgcgca ccacgcgggc gcggtgaggc
     8341 aaccgcgggg tccgttcagc ggctcgccgc cgcggcaggc gccctggccg aggtttgctg
     8401 agcaaacccg cctgcgaagc aggtggccta gtgcttgggc gggctggcgg tatgggcgtt
     8461 gctccacagc agcccgcact cgccgtcggg gtcggatcga tggtcggtgg cacggatacg
     8521 cagcgccccg ggggcgtggc tggtgggcac ccgggcgtgc aggcggccgt cggggccggc
     8581 ggtgtaactg aggtagtcgc tgacgtcgtc ttcgatgtac gtgacgcaga tggtgatgcg
     8641 gcgtcccggg aggaatccct cgcccgtgac gaaatcggcc gtgccggtga tggtcggggc
     8701 caccccacca tcgggtgtcg atctgtcagg cgtggggtcg ctcaacgagg tgctccgatc
     8761 ggctcgcgtt gatgccgacc cgttccggga agccagcatc accaccgcca tctatacccc
     8821 cacctgggtt gctcgaatcg catctggcaa cgcttctccc ggcaaccccg gggtccgctg
     8881 agcggaccac tagacgcccg cgccagaccg tccctcgcgg atcgccccgt gccgcggccg
     8941 gacccgtccg ccgcgaggat tacggcaatc gtgcgctgat gctgcgggcc gcgaatatca
     9001 cctggtcacg gagatcatgc ggcggtctgg cggcctgtac aacggccgtg accgcgccgg
     9061 cacagcttcc gttgggccac cacaccggcg ccgacacgca gcagatgccg gcgacggctt
     9121 cttgatggtc gaccacggtc gcgtgcgcgt cgcggatgcg ggcgcgcagg aggcgccatt
     9181 cgcgtcgcgt ccagcagccc ggaagcgcaa ctccatcggt attcgcttgg gccgcgtaaa
     9241 ggacacgtcc ggtggcggtg cgggcggtcg attctgggtc cgccgggttg ggcagatagg
     9301 cgtggccgtg cggcgcggtg gtacagatcg cccgcaaccg gtcttcatgc aggatccgca
     9361 gcgacgccgt ggcgcgcgac tgcacggcca gggtgtgcac cggcgcctgc cccgcgtcgc
     9421 gcagctgggg gtcgggctgc cagcgttgtc cgatgtgtcc gattcgcgcg ccgacgtagt
     9481 aacggcgctg cacgcgctgg accgcgccga gggccgcgag ctgttcggca agccggtacg
     9541 ccgagctctt gggcagcccg ctggcgcagg ccaactcggt caaccccaga ccgtcggcgg
     9601 cggcgagcgc ctcgaggacc gcgaacgcgc cgtcgagcac ccctcggctg gtacctactc
     9661 cgcgcggggg cgccgccgac gcatccgcga gtccgggcct tcgtttggcg cacgtcggcc
     9721 cggacgaaga atcatcgaca atgctgacaa ccgccgaatg ccccaccata gcgaccactt
     9781 tccccacacc tgaacatctg tcgcgcccga cgttaggtcg ctgagcgcgc gaagacacga
     9841 gtagtgcgct actgcactac ggccgcttgc gtccgggggc tggccacgtc aggcggggca
     9901 gcgcgccacc aactcctgcg gcgtccacgc cggccaggcg ggtgcaccga tcggctgacc
     9961 gccgctcatg tgagccacga tccggggacc cttgagtgca ctgttgtcgt acacggtcgc
    10021 ggtgtcgcac cggatgatcg ccgtgccgac cagggcccaa agacgttggt agcgttgacg
    10081 aatcttgtcc tcgggcacgt cgtgtccacc ggcctcgact cggtgcctga cccgctgcac
    10141 cgccaactct tcggggatga gcaacacgtg cagcacgacg gtgtagcccg cggtttgcgc
    10201 ggtgtcgatc aactcgagtt tggaggggtg agaaaacacg gtttcggcga tgaaggagcg
    10261 gcgcaggtcg atgagccgtg ccctggtgtc ggcggccacc cgggcggcgt cgtacgcgtg
    10321 tgcggccggg tcgtcgggcc agcgctgtct ggcgatctcg tcggcgttga cgtagccgct
    10381 tcgcggcagc agcggcgcga gtgtgagttc gacgaaggtt gacttgccgg ccccgttggg
    10441 tccgacgacg aggtcgagcc ggttcatcgg ccggcggttg tcacttccgg ctacgtgcgg
    10501 gccggcgttt ggccgtcaac acaatcgacg tgccatcggg gcggtgttcg acgatctccc
    10561 ccgcctcgtt ctgggcgacg gtggtgatgc cacgctgggc gagcaactgc ccgtagttgg
    10621 actcggcgag gttctcctgg agggccgcgg cgatttcggc gttgaagacc accccttctt
    10681 cgacgctgag ctcgcgcagt tccaggtctc cggccagcgc cgcctccacg cgccggcgtg
    10741 ccgcggtgtg ctggctcgac acggcccggc caacccgagc ccagtgatcg agttgctgct
    10801 tggccgaccg gctttgccgc gcgccctcga ccgcggcgct gtccagcagg tcgacggcga
    10861 aacgggtcac ccggtcggcg ttgttggcca ccactcctca attctgtagc aaactgtaac
    10921 gagtgtagca gctagacatc ccaggttcgt aagttgtcaa gcggcctggg tgccggagct
    10981 ggtttgacgg tgttcccagg ctcggtgttc gtcgtagtgg gttttgtggc gtaggcagcc
    11041 gtgcaggatg ccgatgaggc ggttgccga
//
LOCUS       DASSGQ010000127        15714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_192000,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000127 DASSGQ010000000
VERSION     DASSGQ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15714
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192000"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="VFI55_05940"
     CDS             <1..145
                     /locus_tag="VFI55_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VII secretion protein EsxI"
                     /protein_id="HET7073609.1"
                     /translation="VACQEFITQLGRNFQVIYEQANAHGSKVQSAGSNMASTDSAVGS
                     SWA"
     gene            complement(286..2289)
                     /locus_tag="VFI55_05945"
     CDS             complement(286..2289)
                     /locus_tag="VFI55_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET7073610.1"
                     /translation="MRIARLLGVFLTIITSGLLLAPHADAQPPFRLPGYVTDNPGLLS
                     DSGRSTVASAVDKLYAERRIRLWVVYVDNFSGLSAENWAKRTYNTSDLGTNDALLAVS
                     STGRAYAFLVPSTVHNISSSQVDDLRHSQIEPALRRGDWSGAAVAAANGLNRAPDYSG
                     RILLLVILGVIAIAVVVFLIVMRRRARRRRAAALAAARRVDPTDANALAAVPLQALDD
                     LSRSMVVDVDNALRTSTNELTLAIDEFGKERTQPFTDAVNNAKAALNQAFTVRQQLED
                     AAPETPTQRRDMLTRVIVSAARADRELDSQTEAFEKLRDLVINAPSQLDGLTQQYVEL
                     TGRIAPAEQRMAELHKEFDDAALSPVSGNVIGAKERLAFADRNISSARELAGHAESGK
                     QTGVVDAYHAAESALGQARSLLDAVDTAADDIRHAVASLPSAMTDIQASIKRADDRLK
                     QTPKSRSPRVKELAAARDAAVTAADSAHGSAATDPLGTFAKLTRADADLTRLLTALAE
                     EQATAERLNRSYEQALFTAESRVRAVSEYIDTRRGSIGPEARTRLAEAKRQLQVAHDK
                     RTTNLLEATAYANGASAWAAYAQSLANADVQSAQRAYSRRRGGGGGNDTGALIGGIII
                     SDLLRGGMRGGSGGGWIPTSFGGSSGSSGGSSDGGFMGGGGRF"
     gene            2368..3645
                     /locus_tag="VFI55_05950"
     CDS             2368..3645
                     /locus_tag="VFI55_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HET7073611.1"
                     /translation="MTTNQQDPYDDFDRQRRVREAPKTAGLPGTEGQHRTDFARDRAR
                     VLHSAALRRLADKTQVVGPREGDTPRTRLTHSLEVAQIGRGMAVGLGCDLDLVELGGL
                     AHDIGHPPYGHNGERALDEFAAMQGGFEGNAQNFRILTSLEPKVLDAQGNSAGLNLTR
                     ASLDAVTKYPWNRGQGIANGRRKFGFYDEDAESAAWVRARAPADRMCLEAQVMDWADD
                     IAYSVHDVEDGVVSQRIDLRVLADDDEAAALARLGESEFSRVSADDFMAAAQRLSGLP
                     VVAAVGKYDATLAASVALKRLTSELVGRFASAAIATTRAAAGPGPLVRYQADLLVPDL
                     VRAEVALLKILALQFIMSDPRHLETQVRQRDRIHRVAHWLYSGAPRTLDPMFAATFNT
                     APDDRARLRVIVDQIASYTEGRLERIDTRQATD"
     gene            3684..4298
                     /locus_tag="VFI55_05955"
     CDS             3684..4298
                     /locus_tag="VFI55_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HET7073612.1"
                     /translation="MTKLTAAAALVLSLPVVAVNACSSPQHASTQPGTTPPVVSGSAS
                     ATTTTSTPTAGESLTAQLNAPDGRQVATATFDFANGYATVTVKTVAAGILAPGFHGLH
                     VHEVGKCEPNSVAPTGGPPGNFLSAGGHYQAPGHTGKPESGDLTSLEVRSDGSAYLVT
                     TTDAFTRDGLLAGNKTALMLHGAEGGDMAMSAERVACGVIGPAS"
     gene            4325..6244
                     /gene="dnaG"
                     /locus_tag="VFI55_05960"
     CDS             4325..6244
                     /gene="dnaG"
                     /locus_tag="VFI55_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET7073613.1"
                     /translation="MAGRIPDRDVAAIRERVRIEEVVGDYVQLRRAGADSLKGLCPFH
                     NEKSPSFHVRPNHGHFHCFGCGEGGDVYAFVQKIEHVSFVEAVELLADRVGHTINYTG
                     PATSVQRDRGSRSRLVAANAAAAEFYAAALESEEAAPARRYLAERNFDPDAARHFGCG
                     FAPSGWESLTKHLQRKGFEFKELEAAGLSREGRRGPMDRFHRRLLWPIRTSAGEVIGF
                     GARRLFDDDPMEAKYINTPETLLYKKSSVMFGIDLAKRDIAKGHQAVVVEGYTDVMAM
                     HLAGVTTAVASCGTAFGDEHLAMLRRLMMDDSFFRGELIYVFDGDAAGRAAALKALDG
                     EQNLAGQSFVAVAPDGMDPCDLRLKSGDGTLRDLVARRTPLFEFAIRTALAEMDLESA
                     EGRVAALRRCVPMVGQIKDSTLRDEYARQLAGWVGWDDVAQVIDRVRGEAKRSKSSVP
                     AGPGAHAPRRVERQPAAARAEPAAARPDPRDPTLWPQRAALKSALQYPALAGPVFDTL
                     TVESFTHPGYAAVRAAIEAAGGTSSGVNGAQWIDAVRRQTASPLTAGLISELGVEPLP
                     ADEEKLPRYIAGVLARLQEVWIGRQIAEVKSKLQRMSPIEQGDEYHALFGDLVAMEAY
                     RRSLLEQASGDDLTA"
     gene            complement(6219..6485)
                     /locus_tag="VFI55_05965"
     CDS             complement(6219..6485)
                     /locus_tag="VFI55_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073614.1"
                     /translation="MIRYFVALGLGYVLGSRAGRRRYEQIAGTYHALTASPLAKSMIE
                     VGRRKIANRISPDTGFVTLAEIDEQTVIVERPAEPALTPSDRRR"
     gene            6648..6720
                     /locus_tag="VFI55_05970"
     tRNA            6648..6720
                     /locus_tag="VFI55_05970"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6681..6683,aa:Asn,seq:gtt)
     gene            6955..7923
                     /locus_tag="VFI55_05975"
     CDS             6955..7923
                     /locus_tag="VFI55_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7073615.1"
                     /translation="MERQRREPLPGEVRLRVRACGVCHTDVLAVEGQRADPSTPIVPG
                     HEIAGVVEAVGPGVTAWHEGDRVGVGFLNGHCGECEACRRGDFVNCTNQQHTGTTVDG
                     GYAEVAYARASGLVRLPDEMDAVDLAPLLCAGLTVYSALLQAGDGALPGALVAIQGIG
                     GLGHLGIQYAAKLGYRVAAIARGSEKASLATQLGADLYIDSKTADPAAALQELGGAAA
                     IVATASNGASMSPLIRGLARRGKLLVVGAAPEPIEVRTGDIIFGTRSVIGSLTGSSIE
                     NEDNLQLAGRHGIRSMNEVMPFSEAPKAYERMMSGQARFRVVLEVA"
     gene            complement(7951..8637)
                     /gene="cysE"
                     /locus_tag="VFI55_05980"
     CDS             complement(7951..8637)
                     /gene="cysE"
                     /locus_tag="VFI55_05980"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HET7073616.1"
                     /translation="MLAAIRRDIQAAKQRDPAAPTTLEVIFAYPGVHAIWAHRISHWL
                     WNRGARLAARTFAELTRILTGVEIHPAAILGAGLFIDHATGVVIGETAEVGDDVTIYH
                     GVTLGGSGGDTGKRHPTVGDRVIIGAGAKVLGAIKIGDDSRIGANAVVVKEVPSSAVV
                     IGVPGQTVSRSGRGGSPDDSMMPDLVGVSLQSLLTRVAKLEALNDGPQNGRVIRPPEA
                     GVWHGEDFSI"
     gene            complement(8640..9572)
                     /gene="cysK"
                     /locus_tag="VFI55_05985"
     CDS             complement(8640..9572)
                     /gene="cysK"
                     /locus_tag="VFI55_05985"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET7073617.1"
                     /translation="MSIAEDVTQLVGNTPLVRLNRVTEGAVADVVAKLEFFNPANSVK
                     DRIGVAMLDAAEQAGLIKPDTIILEPTSGNTGIALAMVAAARGYRLVLTMPETMSVER
                     RMLLRAYGAELILTPGAEGMPGAIAKAEELAKTDQRYFVPQQFENLANPAIHRTTTAE
                     EVWRDTDGKVDIFVSGVGTGGTITGVAQVIKERKPSAQFVAVEPAASPVLSGGQKGPH
                     PIQGIGAGFVPPVLDLDLVDEVITVGNEDALNLARRLATEEGLLVGISSGAAAVAALQ
                     VARRPENAGKLVVVVFPDFGERYLSTSLFADVAE"
     gene            complement(9583..10866)
                     /locus_tag="VFI55_05990"
     CDS             complement(9583..10866)
                     /locus_tag="VFI55_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioester domain-containing protein"
                     /protein_id="HET7073618.1"
                     /translation="MTLLSMPNRALAQVATRRRVLVRPATDVARMMRYRGGTYSHTVD
                     KIVFTDGSWARTDLIRLNPNLLAYSLDFTGIAPHLPSRYRLGTWSTLPHLRTRGHEAE
                     VDWILRNSFPTRSIAELSRRVRAAGHPLGAANLSEHEAIAATQAAIWHFTNGLALDTR
                     PRNVPVAVDRAAGPMITFEFDGHPQLGGYSVSTASATTVGLKLQKSANGVVWQDVSGS
                     HLTIKPGAGRHQRTLGVGSTLSASTHGGGGRGYRYYRLIATTEAADATIGDVRFWLTG
                     ARHYRNTDRIVHLYNYLLIGASKALRNRDEPRLVDTHATADSELIGPFQVRIPLRLSV
                     TDGHTLVDADGLTIDGIVAPGTDFYLRPAPGTSTTTLTATTPHDLTGRVLTGEASAGA
                     AQRFTPIALIVPTDVAVEFDIAWQTDEPDLNSHTA"
     gene            11276..12211
                     /locus_tag="VFI55_05995"
     CDS             11276..12211
                     /locus_tag="VFI55_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment shell
                     protein"
                     /protein_id="HET7073619.1"
                     /translation="MTSAQQGAQNESQALGDLAARQLANATKTVPQLATITPRWLLHL
                     LSWVPVEAGLYRVNRVVNPDQVAIKAEEGAGTEEPLPETYVDYETSPREYTLRTISTL
                     LDIHTRVSDLYSSPHDQVAQQLRLTIETIKERQEFELVNSPEYGLLAQVTPEQTIQTL
                     GGAPTPDDLDALITKVWKTPSFFLTHPLGVAAFGREATFRGVPPPVVSLFGAQFITWR
                     GIPLIPSDKVPVEDGKTKFILVRTGEERQGVVGLFQPGLVGEQAPGLSVRFTGINQSA
                     IATYLVTLYTSLAVLTDDALAVLDDVAVDQFHEYK"
     gene            12198..14108
                     /locus_tag="VFI55_06000"
     CDS             12198..14108
                     /locus_tag="VFI55_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment cargo
                     protein cysteine desulfurase"
                     /protein_id="HET7073620.1"
                     /translation="MSTSEYRAVDAESELPISAAEPAALATQLYAASIRPGPDSPPQT
                     APVAPRGGVPDTTAATSAGRTAAGGADLYQPPVPLIGVGDIYVPAPTSPQPEGPPQAV
                     PVAPRGNVPDTTAAPSAGAATAGVADPYLPPRVGGLGAFAVPSHGIVPTVPGVLAGAA
                     APGKPSVPVAPRGSAPGWPSEAPSVVDLGWSDAVPAAPAALNYAVGDEHSYDFLTKAD
                     RVPRLSDEHEVFDVNAVRADFPILTETINGKPLIWFDNAATTQKPQVVIDRLSYFYAH
                     ENSNIHRAAHELAARATDAYEEARSTVRRFIGASKDEEIIFVRGTTEAINLVAYAWGG
                     KHLLPGDEIVITHLEHHANIVPWQLLSQQTGAVLKVAPVDDAGNLLMSEFEDLLGPKT
                     KLVAATQVSNALGTVTPVEQIVDLGHRYGARVLIDGAQSIQHLPVNVAELGVDFFVFS
                     GHKIYAPTGIGALYGSEEALAETPPWQGGGNMIADVTLERSLYQGPPSKFEAGTGNIA
                     DAVGLGEALRYVERLGIERIAAHEQALLDYATPRLADIPGVRLVGTATEKASVLSFVL
                     AGHDPLEVGKALNAEGIAVRAGHHCAQPILRRLGLEATVRPSFAFYNTFEEIDVFLNA
                     VRRIAEGGAGAG"
     gene            14339..15106
                     /locus_tag="VFI55_06005"
     CDS             14339..15106
                     /locus_tag="VFI55_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7073621.1"
                     /translation="MNGATIAKPMNAVGEFFILSIESLIAVVRGPWAWRELLEQIWFV
                     ARVSIFPTIMLSIPYTVLIVFVLNILLVEIGAGDLSGAGAALASVTQVGPVVTAMAVA
                     GAGSTAMCADLGARTIREEIDAMEVIGVNPVQALVVPRIIAATFVALLLYSVVAVTGL
                     TGSYIFVVFVQHVTPGAFIAGMTLLTGLPQVVISLIKATLFGLSAGLIACYKGLSVGG
                     GPTGVGNAVNETVVFSFMALFFINIVITALGVKVTAK"
     gene            15103..>15714
                     /locus_tag="VFI55_06010"
     CDS             15103..>15714
                     /locus_tag="VFI55_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7073622.1"
                     /translation="MSDAAREPGWFLTIGPRLAAATRRLGEQTEFYGKSLVATVDAVR
                     RYPGELLRIIAEMGMGSGALAVIGGTVGIIGFLTLTTGALVAVQGYDTLSNIGVEALT
                     GFLSAFFNVRMIAPCTAGVALAATIGAGTTAQLGAMRINEEIDALEVMGIRSITYLAS
                     TRIIAGVLVVIPLYAVAVLMSFFAAKFGTLYIYGQSRGVYEHYF"
BASE COUNT         2517 a         5154 c         5340 g         2703 t
ORIGIN      
        1 ggtggcctgc caggagttca tcacccagtt gggtcgtaac ttccaggtga tctacgagca
       61 ggccaacgcc cacggctcga aggtgcaaag cgccggcagc aacatggcca gcaccgacag
      121 cgccgtcggc tccagctggg cgtagcgaag accggttaac ggcggggcgc accggcagtc
      181 cggtgcgccc cgccatccgt tttcgagtcg tacgtgaacc acccccgggt actcggtgcc
      241 cgggggtggt tgcgcttcaa accgatttgt tcggacgtga cgggattaga acctcccgcc
      301 gccacccatg aagccgccgt cggaagagcc tccggatgag cccgaggagc cgccgaacga
      361 cgtggggatc caaccaccgc ccgaccctcc ccgcatgccg cccctgagca ggtcgctgat
      421 gatgatcccc ccgatcagcg cgccggtgtc gttgcccccg ccgccccctc tgcgacggct
      481 gtaagcgcgc tgggccgact gcacatcggc attcgccagt gactgcgcgt aagccgccca
      541 cgccgacgcc ccgttggcgt atgccgtcgc ctcgagcagg ttggtcgtcc gcttgtcgtg
      601 cgcgacttgg agttgccgtt tcgcctcggc cagccgggtc cgagcctccg gaccgatgct
      661 gccgcgacgc gtgtcgatgt actcggagac ggcccgcacg cgcgactcgg cagtgaacaa
      721 agcctgctcg tatgaacggt tgagccgctc ggccgtcgcc tgttcttcgg ccaacgcggt
      781 cagtagccga gtcaggtccg cgtcggccct ggtcaacttg gcgaacgtgc cgagaggatc
      841 ggtggcggcg ctgccgtggg cgctgtcggc ggctgttact gccgcgtcac gcgccgcggc
      901 caactccttg acccgcggag atctgctctt cggcgtctgc ttgagtcggt cgtcggcccg
      961 cttgatactc gcctggatat cggtcatagc cgacggcagc gaggcgacgg cgtgccgaat
     1021 gtcgtccgcc gcggtgtcca cggcgtcgag caacgaacgc gcttgaccca gcgccgactc
     1081 cgccgcgtgg taagcatcca ccacaccggt ctgctttccg ctctccgcgt ggccggccag
     1141 ttcacgggcc gagctgatat tgcggtcggc gaacgccaat cgttccttgg cgccgatgac
     1201 attgccggag accggactca gcgccgcgtc gtcgaattcc ttgtgcagtt cggccatccg
     1261 ctgttccgct ggagcgatgc ggccggtcag ctcgacgtac tgctgcgtga gcccgtcgag
     1321 ctgagacggg gcgttgatca ccaaatctcg cagcttctcg aacgcctcgg tctgtgagtc
     1381 cagttcgcgg tcggcgcgcg ccgccgacac gatcacccgg gtgagcatgt cgcggcgttg
     1441 tgtgggcgtc tcgggcgcgg cgtcttccaa ttgctggcgc acggtgaacg cctggttcag
     1501 ggccgctttg gcattgttca ccgcatcggt gaacggctgg gtccgttctt tgccgaactc
     1561 gtcgatcgcc agggtgagct cattggtact ggtgcgcaac gcgttgtcga cgtccaccac
     1621 catcgaccgc gacaggtcgt cgagggcctg cagcggcacg gcggccagcg cattcgcgtc
     1681 ggtcgggtcg acccgccggg cggcggccag agcggcggcg cgccgtcgcc gggcgcggcg
     1741 gcgcatgaca atcaaaaaga ccaccaccgc gatggcgatg acgcccaata tgaccagcag
     1801 caatatccgg cccgagtaat cgggtgccct gttgagtccg ttggcggcgg cgactgccgc
     1861 accactccag tcgcccctgc gcagcgcggg ttcgatctgg ctgtggcgca ggtcgtcgac
     1921 ctgactggag ctgatgttgt gcaccgtgga cggcaccagg aacgcgtagg cgcgcccggt
     1981 gctggacacg gccagcaacg cgtcgttggt acccaggtcg ctggtgttgt acgtgcgctt
     2041 cgcccagttc tccgcgctca gcccggagaa gttgtcgacg tagaccaccc acagccggat
     2101 gcggcgttcg gcgtagagct tgtcgacggc tgacgccacc gtggagcgcc cggagtctga
     2161 gagcaggccg gggttgtcgg tgacatagcc cggcagccgg aacggtggct gcgcatcggc
     2221 gtgcggcgcc agtagcagcc cgctagtgat gatcgtgagg aacacgccga gcaagcgagc
     2281 gattcgcatg gtggttaatt tagccctgcg acggtgtcaa acctcgctgt gtccttgctg
     2341 gcagactgtg ctgcggtgat taagaagttg actacgaatc agcaagaccc ctatgacgac
     2401 ttcgaccgtc agcggcgggt acgcgaagcg ccgaagactg ctggtctgcc gggcaccgaa
     2461 ggtcagcacc gcaccgactt cgcccgcgac cgagcccggg tattgcacag cgccgcgctg
     2521 cgccggctcg ccgacaagac gcaggtggtc gggccccgcg aaggggacac tccgcggacc
     2581 cggttgacgc actcgttgga agtggcccag atcgggcggg gaatggcggt cgggctgggt
     2641 tgtgacctcg acctggtgga gctcggcggt ctcgcccacg acatcggcca tccgccctac
     2701 gggcacaacg gcgagcgcgc gctcgacgag ttcgcggcca tgcagggcgg cttcgagggc
     2761 aacgcccaga acttccggat cctgaccagc ctggagccca aagtgcttga tgcacagggc
     2821 aatagcgcag gactaaacct gactcgcgca tccctggacg cggtcaccaa gtacccgtgg
     2881 aaccgtgggc aagggattgc gaacgggaga aggaagttcg gcttttacga cgaggacgcg
     2941 gagtcggccg cctgggttcg cgcacgcgcg ccggcggacc gaatgtgcct ggaagcccaa
     3001 gtgatggact gggcggacga tatcgcgtat tcggtgcacg acgtcgaaga cggcgtcgtc
     3061 tcccaacgca tcgacctgcg cgtcctcgcc gatgacgacg aggcggcggc cctggccagg
     3121 ctgggggaga gcgaattctc ccgggtgagc gccgacgact tcatggcggc cgcgcaacgg
     3181 ctgtccggcc tgcctgtggt cgccgcggtc ggcaagtacg acgcgacact tgctgcgtcg
     3241 gtcgcgctca agcggttgac cagtgaactg gtaggcaggt tcgcctcggc cgccattgcg
     3301 accacgcgcg cggcggccgg tccggggccg ctggtgcgct accaggccga cctgctggtg
     3361 cccgacctgg tacgggccga ggtcgcgctg ctgaaaatcc tggcgctgca gttcatcatg
     3421 tccgacccgc ggcatctgga aacccaggtc cggcagcgcg accgcatcca ccgcgtggcg
     3481 cactggctgt actccggggc cccgcgaaca ctcgatccca tgttcgccgc gacgttcaac
     3541 accgcgcccg acgaccgcgc ccggctacgg gtcatcgtcg atcaaatcgc ttcctacacc
     3601 gagggtcggc tggagcgcat cgacacccgt caggcgaccg attagcggtt acttggccgc
     3661 cggctcgggg ttaggctccc gtcatgacca aactcaccgc cgccgccgcg ctcgtgctgt
     3721 cgcttcctgt cgtcgccgtg aacgcgtgca gttcgcctca gcatgcgagc actcagccgg
     3781 ggaccacgcc tccggtcgtc agcggttcag cgtcggcgac gacaacgacc agcacaccga
     3841 ccgccggtga atcgcttacc gcgcaactga atgcgcccga cggccgccaa gtggccacgg
     3901 ccaccttcga tttcgccaac ggctatgcca ccgtcaccgt caagaccgtc gccgccggaa
     3961 tcctggcgcc cggattccat ggcctgcatg tccacgaggt cggcaagtgc gagccgaact
     4021 cagtcgcccc gaccggcggt ccgcccggca acttcctgtc cgcggggggt cactaccagg
     4081 cgccgggaca caccggcaag cccgaaagcg gcgacctgac gtcgctcgag gtgcgctccg
     4141 acggctccgc ctacttggtg accaccaccg atgcgttcac cagggacgga ctattggcgg
     4201 ggaacaagac cgcattgatg ctgcacggcg ccgaaggcgg cgacatggcg atgtcggcgg
     4261 agcgggtcgc gtgcggggtc atcggcccgg ccagctgatc gggtcggcgc tctagactgg
     4321 gccgatggct ggccggattc ccgatcgtga cgtcgcggcc atccgcgaac gggtccgcat
     4381 cgaggaagtc gtcggcgact acgtgcaact gcggcgcgcc ggagccgact cgctcaaggg
     4441 cttgtgccca tttcacaacg agaagtcgcc gtcgtttcat gtgcgcccca atcacggtca
     4501 ctttcactgc ttcggctgcg gtgaaggcgg cgacgtgtac gccttcgtgc agaagatcga
     4561 acacgtcagc ttcgtggagg cggtcgagct gctcgccgac cgggtcggcc acaccatcaa
     4621 ctacaccggc ccggccacca gcgtgcagcg cgaccgcggc agtcgcagca ggctcgtcgc
     4681 ggccaacgcc gccgcggcgg agttctacgc ggcggcgctg gagtccgagg aggcggcacc
     4741 ggctcgccga tacctggcgg agcggaactt cgaccccgat gcggcccgtc atttcggctg
     4801 cggattcgct ccgtccggct gggagtcgct gacaaaacat ttgcagcgca agggttttga
     4861 gttcaaagaa ctggaagccg ccggcctgtc ccgggagggg cgccgcggcc cgatggaccg
     4921 attccaccga cggctgttgt ggccgatccg cacctcggcc ggtgaggtga tcgggttcgg
     4981 agcccggcgg ctgttcgacg acgacccgat ggaagccaag tacatcaaca cgcccgagac
     5041 cctgctgtac aagaagtcgt cggtgatgtt cggtatcgac ctggccaaac gcgacatcgc
     5101 caaggggcac caggcagtgg tcgtcgaggg ctacaccgac gtgatggcga tgcatctggc
     5161 cggggtcacc accgcggtcg cctcctgcgg caccgcgttc ggcgacgagc acctggcgat
     5221 gctgcgcagg ctgatgatgg acgacagctt ctttcgcggc gaactgatct acgttttcga
     5281 cggcgacgca gcgggccgcg ctgccgcgct caaggcctta gacggtgagc agaacctggc
     5341 ggggcagtcc tttgtcgcgg tggcgccgga cggtatggat ccctgcgacc tgcgtctcaa
     5401 gtccggcgac gggacgctgc gtgatctggt ggcacggcga accccgttgt tcgagttcgc
     5461 gattcgcacc gcgcttgccg agatggacct ggagagcgcc gagggcaggg tcgccgcgct
     5521 gcgccgctgc gtgcccatgg tgggtcagat caaggactcc acgctgcgcg acgagtacgc
     5581 tcgccagctc gccggctggg tcggctggga cgatgtcgcg caggtcatcg accgggtgcg
     5641 cggcgaggcc aaaaggtcga agtcctccgt cccggccgga cccggcgccc atgccccccg
     5701 ccgcgtcgag cggcagcccg ccgccgcccg tgccgagcct gccgccgccc gacccgaccc
     5761 gcgtgatccg accctgtggc cgcagcgcgc ggcactcaaa tcggcgttgc agtacccggc
     5821 gttggccggc ccggtgttcg acacgctgac cgtggagagc ttcacccacc ccggctacgc
     5881 ggccgtgcgc gcggccatcg aagcggcggg cggcacgtcg agtggcgtca acggtgcaca
     5941 gtggatcgat gcggtgcgcc ggcagaccgc gtcgcccctg acggcgggcc tgatcagtga
     6001 gctgggggtg gagccgttac cggccgacga ggagaagctg ccccgctaca tcgccggggt
     6061 gctggcccgt ctgcaggaag tctggatcgg ccgacagatc gccgaggtga agtccaaatt
     6121 gcagcgcatg tcgcccatcg aacaaggcga cgagtaccac gcgctgtttg gcgacctggt
     6181 cgccatggag gcgtaccggc gcagcctgtt ggagcaggct agcggcgacg atctgacggc
     6241 gtaagagccg gctcggcagg ccgctcgacg ataaccgtct gttcgtcgat ctcggccagc
     6301 gtcacgaatc ccgtgtcggg tgagatccga ttcgcgatct tgcggcggcc aacttcgatc
     6361 atcgacttgg ccaggggact tgcggtcagc gcgtggtagg tgccggcgat ctgctcgtat
     6421 cggcgacgtc cggctctcga gcccagcacg taacccagtc caagcgccac gaaataccgg
     6481 atcaaagcgt tcctcccgac gatctatagt tccatcctgc ctcactcggc atgggtccgc
     6541 gcacgcgatc tgtgtttagg cgcgcgagct gggcggcgag atctcctggt ccctctggtt
     6601 gggtgcggtt gtgccggtac gctagagtcg tcccgcgatc gggttaatcc cctgtagctc
     6661 aattggcaga gcattcggct gttaaccgaa gggttggagg ttcgagtcct cccgggggag
     6721 cggtgttcgg ttcgcctgtc ccgcgccctg gtcaccctgg acgcgggggt ctcgccggtg
     6781 gcgaatgccg cggccttgag atagtgaact cccagtgaac taggtcccgg gctggcgtcg
     6841 cagacacgac ctggtgcgaa cgtagtaggg gttcgccaca cgcaggagca accaggaggc
     6901 atggtgtcca cgtatcgcgc attcgaggta accggtcgcc gacagtttcg gttggtggag
     6961 cggcaaagac gggaaccgct gcccggtgag gtgcgactgc gggtgcgtgc gtgcggtgtc
     7021 tgccacaccg atgtccttgc agtggagggg cagcgggccg acccgtcgac accgatcgtg
     7081 ccgggccacg agatcgccgg cgtggtggag gcggtaggac cgggagtgac cgcctggcac
     7141 gaaggcgacc gggtgggcgt cggtttcctc aacggtcact gcggtgaatg cgaagcatgt
     7201 cgtcgcggcg acttcgtgaa ctgcacgaac cagcagcaca ccggcaccac cgtcgacggt
     7261 gggtacgccg aggtcgccta cgcacgcgcc agtggactgg tgcgcttgcc ggacgagatg
     7321 gacgccgttg atctggcacc gctgttgtgt gcggggctga cggtctacag cgccttgctg
     7381 caggccggcg acggcgccct gcccggcgcg ttggttgcca tccaaggaat cggcgggctc
     7441 ggtcacctgg gcatccagta cgccgccaaa ctcggatacc gcgtcgcggc aatcgcgcgt
     7501 ggtagcgaaa aggccagcct cgcaacacaa ctcggggcag acctttacat cgacagcaag
     7561 accgccgatc ccgcggccgc gctgcaggaa ctcggcggag ccgcggccat cgtcgcgacc
     7621 gcctccaacg gtgcgtccat gtcgccgcta attcgcggcc tggcccgacg cggcaagctg
     7681 cttgtggtcg gcgcggcccc cgagcctatc gaggtgcgga ccggcgacat catcttcggg
     7741 acgcgctcgg tgatcggctc gctgaccggt agctccatcg agaacgagga caacctgcag
     7801 ttggcgggcc gccacggcat ccgctcgatg aacgaggtca tgcccttttc ggaggcgccc
     7861 aaggcgtacg aacggatgat gtccgggcag gctcggttcc gcgtcgtcct ggaggtcgct
     7921 tagcacgggc ccagttggac ccagctgaac ctagatcgag aagtcctcgc cgtgccacac
     7981 cccggcttcg ggcgggcgga tcacgcggcc gttctggggg ccgtcgttca aggcctccag
     8041 cttggccacc ctggtcagca gcgactggag gctgacgccc acgaggtccg gcatcatcga
     8101 gtcgtccggg ctgccgcccc ggccggaacg gctgaccgtc tgccccggga ccccgatgac
     8161 caccgcgctg gacggaacct ccttgaccac gacggcgttg gcgccgatgc ggctgtcgtc
     8221 gccgattttg atcgcgccca ggaccttggc gcccgccccg atgatgacgc ggtcaccgac
     8281 ggtggggtgc cgtttcccgg tatccccgcc actgccgccg agcgtcacgc cgtgatagat
     8341 ggtgacgtcg tcacccacct ccgcggtttc gccgatcacc actccggtcg cgtggtcgat
     8401 gaacagaccg gcgccgagga tcgcagcggg gtggatttcg accccggtca ggatgcgggt
     8461 gagttccgcg aacgtccggg cggccagcct ggcgccacgg ttccacagcc agtggctgat
     8521 ccgatgagcc cagatcgcat gcacgcccgg gtaggcgaaa atgacttcca gcgtggtggg
     8581 cgcggccgga tcccgctgct tggccgcctg tatgtcacgc cgaatggccg cgagcatggc
     8641 tactccgcca cgtcggcgaa gagtgacgtg ctcagatacc gctcaccgaa gtcgggaaac
     8701 acgacgacaa ccagtttgcc cgcgttttcg ggccgacggg cgacctgcag tgcggccacc
     8761 gcggccgcac ccgaggagat accgaccagc agcccctctt ccgtggccag ccgccgggcc
     8821 aggttcagcg cgtcctcgtt gccgacggtg atgacctcgt cgaccaggtc caggtcgagc
     8881 accggcggga cgaacccggc gccgattccc tgaatcgggt ggggcccctt ttgtccgccg
     8941 gacagtaccg gcgaggcggc cggctcgacg gcgacgaatt gcgccgacgg cttgcgttcc
     9001 ttgatgacct gcgcgacgcc ggtgatggtg ccgccggtgc cgactccgga cacgaagatg
     9061 tcgaccttgc cgtcggtgtc ccgccacacc tcctcggccg tcgtcgtgcg gtggatggcc
     9121 gggttggcca ggttctcgaa ctgctgaggc acgaagtacc gctggtcggt cttggccagt
     9181 tcctccgcct tggcgatggc gccgggcatg ccctcggcgc cgggcgtcag gatcagttcg
     9241 gcgccgtagg cgcgcagcag catccgtcgt tcgacgctca tggtctcggg catggtcagc
     9301 accaggcggt agccgcgtgc cgcggcgacc atcgccagcg cgatgccggt gttgccgctg
     9361 gtcggctcca ggatgatcgt gtcgggcttg atcaggcccg cctgctcggc cgcgtcgagc
     9421 atggccaccc cgatgcggtc cttcacgctg ttcgccgggt tgaagaattc caatttggcg
     9481 acgacgtcgg cgacggcgcc ctccgtgacg cggttcagcc gcaccagcgg ggtgtttccg
     9541 actagctgtg tgacgtcctc ggcgatgctc atgaaacttc ctttaggcgg tgtgtgagtt
     9601 caggtcgggc tcgtcggtct gccaggcgat gtcgaattcg acggcgacat ctgtcggcac
     9661 gatcagggca atgggggtga atcgctgtgc ggccccggcc gatgcctcgc cggtcaacac
     9721 ccgcccggtg aggtcgtgcg gtgtcgttgc ggtcagtgtc gttgtggagg tacccggtgc
     9781 cggacgcaga taaaagtctg tcccgggcgc gacgatgccg tcgatcgtca aaccgtcggc
     9841 atcgacgagc gtgtgcccgt cagtgacgct gagcctcaat ggaattcgca cctggaatgg
     9901 tcctatgagt tcggaatcgg cggttgcatg ggtatcgact agtcgcggct cgtcacggtt
     9961 gcgcagggct ttgctcgccc caatcagtaa gtaattgtag aggtgcacga tgcggtcggt
    10021 gttgcggtag tggcgcgcac cggtcagcca gaaccggaca tcgccgatcg tcgcgtcggc
    10081 ggcctcggtc gttgcgatca gccggtagta gcggtaaccg cgcccgccgc cgccgtgggt
    10141 gctggccgac agtgtgctgc cgacgccaag tgtgcgctga tgccgcccag cgccgggttt
    10201 gatggtcagg tgtgagcccg aaacatcttg ccagacaacg ccattggccg acttttgcag
    10261 cttgaggccg acggttgttg cggaggcggt cgacaccgag tacccaccca gctgtggatg
    10321 gccgtcgaat tcgaaggtga tcatcggtcc tgccgcgcgg tccacggcga cgggcacgtt
    10381 gcgcggcctg gtgtccagcg ccagaccgtt ggtgaaatgc cagatcgcgg cctgggtcgc
    10441 cgcgatcgct tcgtgttcgc tgaggttggc cgctcccagg ggatggccgg cggcgcgcac
    10501 tcgtcggctc agttccgcga tggagcgcgt cgggaaggag ttgcgcagga tccagtccac
    10561 ttcggcctca tggccgcggg tgcgcaggtg gggcagggtg gaccaggtgc ccaggcggta
    10621 gcgcgacggc aggtgtggcg cgatgccggt gaagtccagc gaataggcca gcaggttggg
    10681 gttcagccgg atcaggtcag tgcgtgccca gctgccgtcg gtgaacacga tcttgtcgac
    10741 ggtgtgagag taggtgccgc cccggtaacg catcatgcgg gcgacgtcgg tggccggccg
    10801 taccagtacc cggcggcgcg tcgccacctg cgccagggcg cggttgggca tcgatagcaa
    10861 agtcatgggt tgtcgatctt cctgaagaat gtggctcggc gcgtctcatg ccgccggggg
    10921 gaccgccggt ccaacccgcg agcggggtga gcgcgaggat ttctagagcg tcagcgggcg
    10981 tcaggaaatc aacaacagca acaacaacag tccacggcga ggcagcgcga aggaatgaag
    11041 cgcagctgaa cagcctgttg catggccccc actatagggc cccgcaagat cactgtcaga
    11101 tttcgggccc gtttcgttga tttcactcac gggcggtgta aaccttcttc ccgggcttta
    11161 cacttgccta cgctcggcgc gcaggacccg tcagggtggg cgatcattga gcttgcggcg
    11221 ccccactaga acattcgaca ttcgacagca ttcgactgag cacggaagga caacgatgac
    11281 gtcggctcaa cagggggctc aaaacgagtc tcaggcactt ggcgatctcg ctgcccggca
    11341 gcttgccaat gcgacgaaga ccgtcccgca gctcgccacc atcacgccgc gctggctgct
    11401 gcatctgctc agctgggttc cggtggaagc gggcctttac cgggtcaacc gagtcgtcaa
    11461 ccccgatcag gtcgcgatca aggccgaaga gggtgccggt accgaggaac cgctgcccga
    11521 gacctacgtc gattacgaga ccagcccgcg cgaatacacg ctgcggacga tctcgaccct
    11581 gctcgacatc cacacccgag tctccgactt gtactccagc ccgcacgacc aggtagccca
    11641 gcagctgcga ctaacgatcg aaaccatcaa ggagcgccag gaattcgagc tcgtcaacag
    11701 ccccgaatac gggctgctgg cgcaggtcac cccggagcag acgatccaaa cgctgggcgg
    11761 ggccccgacg ccggacgacc tggacgcact gatcaccaag gtctggaaga caccgagctt
    11821 tttcctcacc caccctctcg gcgtcgccgc gttcggccgt gaggccacct tccggggcgt
    11881 gccaccgccc gtcgtgagcc tcttcggcgc gcagttcatc acctggcggg gtattccgct
    11941 gattccgtcg gacaaggtgc cggtggaaga cggcaagacg aagttcatcc tggtgcgcac
    12001 cggcgaggag cgccagggcg tggtgggtct gttccagccg ggcctggtcg gcgagcaggc
    12061 gccgggactg tcggtgcggt tcaccggcat caaccagtcg gcgatcgcca cctacctggt
    12121 cacgttgtac acgtccttgg ccgtcctcac cgacgacgcg ctcgccgtgc tcgacgacgt
    12181 cgccgtggac cagttccatg agtacaagtg agtaccgagc ggtagacgcc gaaagcgagt
    12241 tgcccattag cgccgctgag cccgcggcgc tggccaccca gttgtacgcg gccagcatcc
    12301 gcccggggcc cgacagcccg ccccagaccg caccggtcgc cccgcgcggc ggggtgccgg
    12361 ataccaccgc cgccacgtcg gccggccgga ccgcggcagg cggagccgac ctgtaccagc
    12421 cgccggttcc gctgatcggc gtcggggaca tctacgttcc ggcaccgacg tcaccgcagc
    12481 cggaaggccc gccgcaggcg gtgcccgtgg ctcctcgcgg caacgtgccc gataccaccg
    12541 cggcgccctc ggccggcgcc gcgacggccg gcgtcgccga tccctacctg ccgccccgcg
    12601 tcggtggcct cggcgccttc gcggtcccca gccacggcat cgtgccgact gtgcccggcg
    12661 tgctggccgg ggccgcggct cccggcaagc cgagcgtgcc ggtggccccg cgcggttccg
    12721 cgcccgggtg gccgtccgag gcgccgtcgg tcgtcgacct cggttggtct gacgcggtgc
    12781 ccgccgcgcc ggccgcgctg aattacgcgg tcggtgatga gcacagctat gacttcctga
    12841 ccaaggccga tcgggtaccg cggttgtccg acgagcacga ggtgttcgac gtcaacgcgg
    12901 tacgcgcgga ctttccgatc ctgacggaga cgatcaacgg caagccgctg atctggttcg
    12961 acaacgcggc gaccacccag aagccacagg tcgtgatcga tcggctgtcg tacttctacg
    13021 cccacgagaa ctccaacatc caccgcgcgg cgcacgagct cgcggcccgc gccaccgacg
    13081 cctacgaaga ggcgcgcagc accgttcggc ggttcatcgg cgcgtcgaag gacgaagaga
    13141 tcatcttcgt gcgcggcacc accgaagcca tcaacctggt tgcttatgcc tggggcggca
    13201 agcatttgct acccggcgat gaaatcgtta ttacacatct ggagcatcac gccaacatcg
    13261 tcccgtggca gctgctttcg cagcagaccg gtgcggtcct gaaagtcgct ccggtggacg
    13321 acgccggcaa tttgctgatg tcggaattcg aagacttgct ggggcccaag acgaaattgg
    13381 tcgccgccac tcaagtgtcc aacgcgctgg gcacggtgac gccggtggag cagatcgtcg
    13441 acctgggcca ccgctacggc gcgcgggtgc tgatcgacgg tgcccagtcg attcagcatc
    13501 tgccggtcaa cgtcgccgag ctcggtgtgg atttcttcgt cttctccggc cacaagatct
    13561 atgcccccac cgggatcggt gcgctctacg gaagcgagga ggccctcgcc gagactccgc
    13621 cgtggcaggg cggtggcaac atgatcgccg atgtgaccct ggagcggtcg ctgtatcagg
    13681 ggccgccgag caagttcgag gccggcaccg gcaacatcgc cgacgccgtc gggctcggcg
    13741 aggccctgcg gtacgtggag cggttgggca tcgaacgcat tgccgcccac gagcaggcgc
    13801 tgctggacta tgcgacgccg cggctggccg acatcccggg tgtccgcttg gtgggcaccg
    13861 caacggagaa ggccagcgtg ctgtcgttcg tgctggccgg acacgacccg ctcgaggtgg
    13921 gcaaggcgct caacgccgag ggcatcgcgg tgcgtgccgg ccatcactgc gcgcagccga
    13981 ttctgcgtcg cttgggcctg gaagccaccg tgcgcccctc gtttgcgttc tacaacacgt
    14041 tcgaggagat cgacgtcttc ctcaacgcgg tacgccggat cgccgagggt ggcgcgggcg
    14101 ccggctgagc ttagccggat ttcttcttcc tgatttcaac cctgcgactt gtcgcgggtt
    14161 gctcgtagac tgccgaagtg ttttctacgt gcaattaggc ggagtggccg cattgcgcga
    14221 tgaataccgc taatcgtttg cggtaacacc aattacgagt ttttcgtttt attcacgtaa
    14281 acggggggcc tatgaggcgt atgggtatct gcacgctccg ggagaccgaa caggacacat
    14341 gaacggcgcg accatcgcaa agcccatgaa cgcggtgggc gagttcttca tcctcagcat
    14401 cgaatcgctg atcgctgtgg tgcgagggcc gtgggcgtgg cgcgagcttc tcgagcagat
    14461 ttggtttgtc gcgcgggttt cgatttttcc gacgatcatg ctgtcgattc cgtacacggt
    14521 gctcatagtg ttcgtcctga acatcctgct ggtcgagatc ggcgcggggg acctctcggg
    14581 tgccggtgcc gcgctggcgt cggtgacgca ggttgggccg gtcgttaccg cgatggccgt
    14641 cgccggcgcc gggtcgaccg cgatgtgcgc tgacctggga gcccggacca tccgcgagga
    14701 aatcgacgcg atggaggtga tcggcgtcaa tcccgttcag gccctggtgg ttccgcgtat
    14761 catcgcggcc acgttcgtcg cattgctgct ctattcggtg gtcgcggtca ccgggctgac
    14821 gggcagctac atcttcgtgg tcttcgttca gcacgtcaca ccgggcgcct tcatcgcggg
    14881 gatgacattg ctcacggggc taccgcaggt cgtgatctca ctcatcaagg caaccctgtt
    14941 cggtctgtcc gccggcctga tcgcgtgcta caagggcctg tcggtgggcg gcggccccac
    15001 cggtgtcggg aatgcggtca acgagacggt ggtgttttcg ttcatggcgt tgttcttcat
    15061 caacatcgtg atcaccgcgc tgggtgtgaa ggtgactgcc aaatgagcga tgcggcaaga
    15121 gaacccgggt ggttcctcac tattggaccc aggctcgcgg cagccacccg gagattgggc
    15181 gaacagaccg agttttacgg gaaatcgctg gtcgccaccg tcgacgcggt gcggcgctac
    15241 ccgggcgagc tgctacggat tatcgccgag atgggtatgg gcagcggtgc gctggcggtg
    15301 atcgggggaa ccgtgggcat catcggcttc ctcacgctca ccaccggtgc cctggtcgcg
    15361 gtgcagggct acgacacgtt gtccaacatc ggggtcgagg cgctgacggg atttctgtcg
    15421 gccttcttca atgtccgcat gatcgcgccg tgcacggccg gggtggcgtt ggccgccacg
    15481 atcggtgccg gcacgacggc gcagttgggc gcgatgcgca tcaacgagga gatcgacgcg
    15541 ctggaagtca tgggtatccg ttcgatcacc tatctggcgt cgacgcggat catcgccggc
    15601 gtcctggtcg tcatcccgct ttatgccgtc gcggtgctga tgtcgttctt cgcggccaag
    15661 ttcggcacgc tgtacatcta cggccaatca cgcggcgtct acgagcatta cttc
//
LOCUS       DASSGQ010000128        12158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_920960,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000128 DASSGQ010000000
VERSION     DASSGQ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12158
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920960"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            616..891
                     /locus_tag="VFI55_06015"
     CDS             616..891
                     /locus_tag="VFI55_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HET7073623.1"
                     /translation="MGLRGRFSSIIAFLRAGYPSDAPPTGYIPLLALLPRRLSDDEAA
                     RIATKLFWHKRRSFDRADVGVEITRLTDRMPSIEDIERVEHRLDAMG"
     gene            complement(920..1546)
                     /locus_tag="VFI55_06020"
     CDS             complement(920..1546)
                     /locus_tag="VFI55_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009312 - oligosaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009877 - nodulation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET7073624.1"
                     /translation="MTARLPDAYFDRMYAGADDPWQLSTRWYEQRKYAITLALLPDRR
                     YRHAFEPGCSIGTLTAQLVRRCDHVTAVDVADAALRSADARLRRAGCRDRVTLYQSSL
                     DVTWPPGPFDLLVLSEVAYYLDADALATVLGRECPRLQRGAKVVAAHWRHAVTDYPLT
                     GDAAHAVIAETPGLTSVARYGDRDVVIEVFDTGDGRSVAAREGVPGAR"
     gene            complement(1543..2310)
                     /locus_tag="VFI55_06025"
     CDS             complement(1543..2310)
                     /locus_tag="VFI55_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HET7073625.1"
                     /translation="MKTASTGNCARFAAKPLTRGGTPVPVWLAAFGADPPPELDLTDC
                     PGLIIVAPHPDDETLGLGATTAQLVASGVDVKVVSVSDGGAAGATLSDRTRLETTRRY
                     ELRRATNVLGVAPPVSLGLPDGRLADHEDRLAELLGEILAAAAPGTWCAATWRGDGHP
                     DHEAVGRAAAWACAHTGVTLMEYPVWMWHWASPGDPAVPWDRGHRVRVSGWALGRKRD
                     AAQCFRSQFKTNGAGTAPVLPAFVLQRLLSVGEVVFR"
     gene            complement(2329..3270)
                     /locus_tag="VFI55_06030"
     CDS             complement(2329..3270)
                     /locus_tag="VFI55_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7073626.1"
                     /translation="MTAGLVMHWLDTGRLELPLPASGRTGERWQRLARLAEDDIAAAR
                     IAEAHVDAVAILHELGGKPPDSGQLWGVWAAESPDAVVTATDVGGGAVILNGTKVWCS
                     GAGFCTHALVTARLDDKSRGLFAVTVIDPAVKALPTTWWNVGMAGSDTRAVQFNNAHA
                     VAVGDPDDYLDRPGFWHGAIGVAACWLGGARRVAEPLYRCARSQSADAYSLAHLGAVD
                     AALAAGDAILCAAAMQVDADPFDRTGTAQLLARRARTVVEHAVDEAITRSGRALGPGP
                     LCQDGRHAQRVADLTIYIRQSHAERDLAELGRLAGRH"
     gene            complement(3267..3992)
                     /locus_tag="VFI55_06035"
     CDS             complement(3267..3992)
                     /locus_tag="VFI55_06035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7073627.1"
                     /translation="MSAPVSRVYDGAAIVIPAHNERAKLPACLRAVLTAALCAPVPVV
                     IVVVLDANDDDSETLAGQYGPDVHFVRVDAHNVGAARAAGFGYARSLCSDDGKCWYAT
                     TDADSRVDPGWLVGQLGLGADMVLGVVRVGDWRAHSADVADRFAQAYEAGPPGDTSDH
                     KHIHGANMGFSARAYWRIGGFRALSSGEDVDLVERFEAAGYTIHRDIELSVITSARTR
                     ARAPHGFAQYLRRLGGSVAGDRA"
     gene            complement(4230..4487)
                     /locus_tag="VFI55_06040"
     CDS             complement(4230..4487)
                     /locus_tag="VFI55_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HET7073628.1"
                     /translation="MPNPSIKNEKMYRELRKQGDSQEKAARISNAAAARGKSSVGRKG
                     GKSGSYQDWTVPQLRKRAKELGMSGYSSLTKDKLIAKLRNH"
     gene            4751..5185
                     /locus_tag="VFI55_06045"
     CDS             4751..5185
                     /locus_tag="VFI55_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7073629.1"
                     /translation="MVEIHVERTIDAPVERVFDWLAEPANLAAAPLALKAGYAKDSPG
                     AGAGALRQVTGVGTWFREKITAYDRPRSYSYLIVRSFPPFNHEGGTLTFTPSGDGTHV
                     DWLSNYTHPAWAGGKLLEAVSYRLLRSSFLAILDACAKALGK"
     gene            complement(5215..6570)
                     /locus_tag="VFI55_06050"
     CDS             complement(5215..6570)
                     /locus_tag="VFI55_06050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7073630.1"
                     /translation="MSPLSRRDFFAATASLVGAGTAAACAADRRPPPDNKSVLIVGAG
                     MAGLSAARTLADAGRPVRVIEARDRIGGRVCTNRDWGAPLEMGASWIHGTSDNPLMEL
                     AHQAQAQLVPTDYYGWAKLAVDPRLGPLDYDSKTWRKFVERARDHVDGGSLGAAVSAA
                     ASSAELSDSDRAQLAFYLTTEIEDEYATDANQLSANTFDKGEYAGGEQDVVTSGYDAL
                     PRLLAGGLEIVLNTPVTAIALRDGSVIVRAGNRSFEGPAAVVTVPLGVLKSGAITFDP
                     PLPDEHSHALHALGFGVLSKSYFRFDQRTWNIENAFYQFIGTEPGLWAQWFTLPGAAG
                     PIVLAFNAGDRGRSVESSSPADLMTSALPIARQLFGDNISPVEVRTSNWSVDPYARGS
                     YSFHAPGSGLEDRRRLQEPIGDRLYLAGEAVGVNNPATVTGAVVSGRHAAAQLMQRLG
                     A"
     gene            6754..7728
                     /locus_tag="VFI55_06055"
     CDS             6754..7728
                     /locus_tag="VFI55_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010924623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7073631.1"
                     /translation="MSAGQSTRKTERLPDVLPPSRVLTVRAADGTPLHTEVFGPADGY
                     PIVLTHGITCAMRAWAHQIADLATDYRVIAFDHRGHGRSGVPRRGGYSLKRLASDLDS
                     VLEATLAPRERAVLAGHSMGGITIAAWSAQYRHQVHRRADAVALINTTTGDLVREVKL
                     LPVPRPLRGLSTARVFAARSLINVFGGFPIPPAAGIPSRYLVTMLAVGKDADPGAARL
                     VHELFVQTPPSGRGGCAKMLVGALGSRYLDLSGLTVPTLVIGSERDRLTPISQSRRIA
                     RTAPNVVGLVELAGGHCSMLEQPGEVNRQLRALVESVTHGVGAGRISS"
     gene            complement(7723..9069)
                     /locus_tag="VFI55_06060"
     CDS             complement(7723..9069)
                     /locus_tag="VFI55_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HET7073632.1"
                     /translation="MTTPPRTADVVVIGAGFAGLAAARELTRRGHEALVLEGRDRVGG
                     RSFTGSVAGLPADLGGTFVGPTQDAVLALAAELQVPTTPTYHDGKNLIHWRGWARTYH
                     GTIPKLSLTGLLDIGRLRWRFDRIARSIPLATPWDARRARELDGMSLGDWLRSVHASD
                     SSRDLLAIMARVTWGCEPDDVSMLHASRYAHAAGGLDRLLDVEDGAQQDRFPGGTQQI
                     AEAAAAELGARVVLNAPVRRIDHHGAGVTVTSDVGQAGARFVIVAIPPAHRASIDFAP
                     RLPDEYVELAQHWPQGRLSKAYAAYETPFWRADGFSGQALSDQGPVFITFDVSPHADG
                     PGILMGFVDARAFDSLPTEQRRRDALRCFARLFGDAALKPLDYVDHRWGAEDFAPGGP
                     TAAVPPGSWTRFGPWLRKPVGPIHWAGTETADEWTGFLDGAVRSGQRAAAEVAALL"
     gene            complement(9148..10272)
                     /locus_tag="VFI55_06065"
     CDS             complement(9148..10272)
                     /locus_tag="VFI55_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073633.1"
                     /translation="MPQLLGPLDEFPVHQLPQPIAWPGPSDRNFYDRSYFNAHDRTGN
                     IFFISGIGYYPNLGVKDAFVLIRRADTQTALHLSDAIDSDRLNQHVNGYRVEVIQPLR
                     KLRLVLDETEGIAADLTWEGLFDVVQEQPHLMRSGNRVTLDAQRFAQLGTWSGRIAVD
                     DAEIAVDPGTWLGSRDRSWGIRPIGEPEPAGRPADPPFEGMWWLYVPMAFDDFAVVLI
                     IQEEPNGFRSLNDCTRVWRHGRVEQLGWPRLKIHYRSGTRIPTGATIEATTTDGTPVH
                     FDVESKLPVPIHVGGGYGGDSDWLHGMWKGEKFVQRLTYDMNDPAIIARSGFGVIDHV
                     GRAVCRDGDGVPVEGWGLFEHGALGRHDPSGFTDWLTVAP"
     gene            complement(10272..11408)
                     /locus_tag="VFI55_06070"
     CDS             complement(10272..11408)
                     /locus_tag="VFI55_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET7073634.1"
                     /translation="MATEPAIHDVEHLQRSSRDVTTLPAVMSHWLSTVLPGGALPEVT
                     VESGVDSTGMSSETIILTARRQQDGQSIEEKLVARVAPTAEDVRVFPTYRLDHQFEVI
                     RLVGELTDVPVPRVRWLENTGDVLGTPFFLMDYVDGVVPPDVMPYTFGGNWFADAPAE
                     RQRELQDATVGVLASLHSIPNAEKTFAFLSEGRRGDTALRGHFNWVRSWYDFAVPDIG
                     RSPLLERTFAWLEANWPQEVAAHEPVLLWGDARVGNVLYRDFRPVAVLDWEMVTLGPR
                     ELDVAWMIYAHMVFQELAGLATLPGLPEVMREDDVRATYRRLTGVEVGDLNWFYVYSG
                     VMWACVFMRTGARRVHFGEVEKPDDVESLFYHAGLMKRLIGEER"
     gene            11466..12068
                     /locus_tag="VFI55_06075"
     CDS             11466..12068
                     /locus_tag="VFI55_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7073635.1"
                     /translation="MKADPSTLDKAPGAGRPRDRRIDSAILAATAELLVQIGYSNLSL
                     AAVAERAGTTKSALYRRWSSKAELVHEAAFPVAPTALEAPAGDIAADIRMMVEATRDV
                     FTTPVVRAALPGLVADMTADPALNERVMSRFTDLFTAVRVRLREAVDRGEAHPDVDPD
                     RLIELIGGATMLRMLLRPEEELDDTWVEQTTAIVVHGVTG"
BASE COUNT         1953 a         4071 c         4136 g         1998 t
ORIGIN      
        1 caatcccccg agcaggcagc cggtgctgat gccggccact cgcccgcgcg cgacgatact
       61 gctgatattc atgatcggtg ttgtccttca aaattgatcg caattgatac gtcgaatgag
      121 gttcccgaaa cctaaatcca aactagcgga cgcggacggt gccgatgttc gctcacaacg
      181 aacgcgcgcc ctatcgcgcc gacggatgtg gccacccaac ggggtttttg ctgcgtgagc
      241 aatgacgttc ttagaagact tagaagaaac gtgatctcga tcgtgatgca atcgtgaccc
      301 gaatcccggc cgggtggcga cgccgacgtc ggcgaactca gtgcgctccc gctgcgagtg
      361 acctgagtcg attcgcgagg gtcccgtccc gccttggggc cgggcccatt cgtctgcgcc
      421 aaaatcctgt gtcggcaatg cattagacgc ggcgccggcg ccgggcgtca cgccaagatt
      481 cttgcccggc gcggaaattg atcaacagat ccgaaaagtc atcgcctcct gctggtttca
      541 aggcgctacg ctgggctatt cctggtgagg ggcgcccgct gatccgcggc gtgcggcggg
      601 ttggcgaggt ggaatatggg tttgcggggg cggttttcga gcatcatcgc gtttctgcgt
      661 gccggttacc cgagtgacgc gcctcccacc ggctacatac cgctgttggc gctgttgccg
      721 cggcgattgt ccgacgacga ggccgcaagg atcgcgacca aacttttttg gcacaaacgc
      781 cgctcgttcg accgggcaga tgtcggggtc gagatcaccc gactcaccga ccggatgccg
      841 tcaatcgaag acatcgagcg cgtcgaacac cgactcgatg cgatgggata agccggtcgg
      901 caccgacgtc ccgctcgtgc tagcgagctc cgggcacccc ttcgcgggcc gccaccgacc
      961 ggccgtcgcc ggtgtcgaac acctcgatga cgacatcgcg gtccccgtaa cgggcgacgg
     1021 acgtcagccc gggcgtttcc gcgatgacgg cgtgcgccgc atcgccggtg agcggatagt
     1081 cggtcacggc gtggcgccag tgtgcggcaa cgactttcgc accccgctgc agccgggggc
     1141 attcgcggcc caacaccgtg gccagcgcgt cggcgtccag gtagtacgca acctcgctga
     1201 ggaccagcag gtcgaaggga ccggggggcc atgttacgtc cagggacgac tgatacaggg
     1261 tcacccggtc ccggcagccg gcccgccgga gccgcgcgtc cgcgcttcgc agtgccgcgt
     1321 cggccacgtc caccgccgtg acgtgatcgc agcgccgcac cagctgcgcg gtcagcgtcc
     1381 cgatcgaaca gcccggctcg aatgcgtgtc gataccggcg gtcgggcagc agcgcgagcg
     1441 tgatggcgta cttgcgctgc tcataccacc gggtcgacag ttgccacggg tcatcggcac
     1501 cggcgtacat ccggtcgaaa taggcatcgg gcagccgcgc ggtcaccgga ataccacctc
     1561 gcccaccgag agtaggcgct gcaatacgaa cgccggcagc acgggtgccg tgccggcgcc
     1621 gttggtcttg aactgactgc gaaagcattg cgcggcatcg cgtttgcgac ccagcgccca
     1681 gccggacaca cgcacccggt ggccgcggtc ccaaggcacc gcggggtcgc cggggctggc
     1741 ccagtgccac atccacaccg gatattccat cagcgtgacc ccggtatgcg cgcacgccca
     1801 tgccgccgcg cgtcccacgg cctcgtgatc ggggtgtccg tcgccccgcc aggtggccgc
     1861 acaccaggtc ccgggtgcag cggccgcgag gatctccccg agcaactcgg ccagcctgtc
     1921 ctcgtggtcg gccagccgcc cgtcgggcaa gcccagggac acgggtggtg caacccccaa
     1981 aacgtttgtt gctctgcgta gttcgtatct tcgggtggtc tccaaacgag tccggtccga
     2041 cagcgtcgcg ccggcggcac cgccgtcgct gaccgacacc acctttacgt cgacgcctga
     2101 cgcgacaagt tgtgcggtcg ttgcgcccag gccgagggtc tcgtcgtcgg ggtggggcgc
     2161 gacgatgatc agaccggggc agtcggtcag atccagttcc ggcggcggat ccgcgccgaa
     2221 tgcggccagc cacaccggca ctggcgtgcc gccccgcgtc agcggcttgg ccgcaaaccg
     2281 ggcgcagttg cctgtcgatg ctgtcttcaa gtcgtcctcg cgtcgtcctt aatgccgccc
     2341 ggcgaggcgc ccaagctcgg caaggtcccg ctcggcgtgg ctttgtcgta tgtagatggt
     2401 caggtcggcc acccgttgcg cgtgccggcc gtcctgacac agcgggcccg gccccagggc
     2461 tcggccgctc cgggtgatgg cctcatcgac cgcatgctcc acgacggtgc gggcacgacg
     2521 ggcgagcagt tgagcggtac cggtccggtc gaagggatcg gcgtcgacct gcattgcggc
     2581 cgcacacagg atcgcgtctc cggcggcaag cgcggcgtcc accgcaccga ggtgcgccag
     2641 cgagtacgcg tcagccgatt ggcttctggc gcagcgatat agcggctcgg cgaccctgcg
     2701 cgctccaccc agccagcagg cggccacgcc gatcgcgccg tgccagaatc ccggccggtc
     2761 caggtagtca tcgggatcgc ccacggcaac cgcgtgcgcg ttgttgaact gcaccgccct
     2821 ggtgtcgctg ccggccatcc cgacgttcca ccacgtggtc ggcaacgctt tgacggcggg
     2881 gtcgataacc gtcaccgcga acaggccccg gcttttgtca tcgagccggg cggtcaccag
     2941 cgcgtgggtg cagaacccgg cgcctgagca ccacaccttg gtgccgttga ggatgaccgc
     3001 gccgccgccg acgtcggtgg cggtcaccac cgcgtcgggc gactctgccg cccagacgcc
     3061 ccacagctga cccgaatcgg ggggcttgcc gcccagctcg tggagtatcg cgacggcgtc
     3121 gacgtgggcc tcggcgatcc gggccgccgc gatgtcgtcc tcggccagcc gcgccaggcg
     3181 ttgccagcgt tcgccggtac gcccagaagc gggcaatggc aattcgagtc gtccggtgtc
     3241 cagccagtgc attaccaacc ccgcggtcac gcgcgatcac ccgccactga tccccccagt
     3301 cgcctgagat attgagcgaa tccatgtggg gcccgggccc gggttcgcgc cgaggtaatg
     3361 accgataatt cgatgtcgcg atgaatggtg tagccggcgg cctcgaaccg ctcgaccagg
     3421 tcgacgtcct cgccggacga cagcgcgcga aagccgccaa tccgccagta agcccttgcg
     3481 ctgaagccca tgttggcgcc gtgaatgtgt ttgtgatcgc tcgtgtcgcc gggcgggccc
     3541 gcttcgtagg cctgcgcaaa acgatcggcg acgtcggcgg aatgggctcg ccagtcgccg
     3601 acccgtacca cgcccagaac catgtccgcg ccgagcccca gctgacccac cagccagccg
     3661 gggtccaccc ggctgtcggc atcggtggtg gcgtaccagc atttgccgtc gtcggagcac
     3721 agcgagcggg catagccgaa gccggccgcg cgcgcggcgc cgacgttgtg tgcatcgacg
     3781 cggacgaagt ggacgtccgg cccgtactgg ccggccaatg tctcgctgtc gtcgtcgttg
     3841 gcgtccagca ctacgacgat caccaccggg accggtgcgc acagggccgc ggtcagcacc
     3901 gcccgcaggc aggcgggcag cttcgcccgt tcgttgtggg cgggaatcac gatggcggcc
     3961 ccgtcgtaca cgcgagacac tggggctgac ataactgaag gcttgcccac taacgccgct
     4021 aattaaacgg gtgacgaacg cgctatcggt caggttgccc gcagccacag cggggaagct
     4081 gcgctccctc ggcgaccatc actgcgactt cacagcggtg cttcccgccg cgcgggccgt
     4141 ccgcgagcgg ctgggactgg gagccgatca acgccctcgc gttcttggtg ctggccggcc
     4201 gactccgcgt cacgggctga cggggtgcgt cagtgattac gcagtttggc gatcaatttg
     4261 tctttggtca ggcttgaata tcctgacatg ccaagttctt tggcccgctt cctcaattgc
     4321 ggaaccgtcc agtcctggta ggaaccggac ttgccgccct tgcgcccgac cgacgactta
     4381 ccccgcgcgg cggccgcgtt ggagatccgc gcggccttct cctgtgagtc accctgcttg
     4441 cgcagctccc gatacatctt ctcgttcttg atggacggat tcggcatggc tcgtcctcct
     4501 ctggtgacac cttcccaagg atgccccgct cggcagcggc cgaaacgcac gcctggcgcg
     4561 cagtggaacc ccggtacccc tctagcacca cgccggctgc cggctgagtg atcccggcgc
     4621 actatttctc cacgcgcaca aagcgattca gcgccagcca cgttcaaggg aagagggtgc
     4681 cgatggggtt cggcggggcg gcgtctagca tgggcgtaga caatgttgtt gctggctggc
     4741 gggtgatctg atggtcgaga ttcacgtgga gcggacgatc gacgcacctg tggagcgggt
     4801 cttcgactgg ctggccgagc cggcgaacct ggccgccgct ccgctggctc tgaaggccgg
     4861 ttatgccaag gactcgccgg gggccggcgc gggagcgctg cgccaggtga ccggcgtcgg
     4921 gacctggttt cgggaaaaga tcacggctta cgatcggccc cgcagctact cctacctcat
     4981 cgtccgatca ttcccgccgt tcaatcacga gggcggcaca ctgacgttca ccccgtcggg
     5041 tgacggcacc catgtggatt ggctgagcaa ctacacccat ccggcgtggg cgggagggaa
     5101 gctgctggaa gcggtcagct atcggctgct gcgctcgagc ttccttgcga tccttgatgc
     5161 ctgcgcaaag gcgctgggga agtagcagcg aatactgctg taggacaacg gtcgtcaagc
     5221 acccaatcgc tgcatcagtt gggcggcggc atgtcggcca ctgaccacgg cgccggtgac
     5281 ggtggccgga ttgttgacgc cgacggcctc gcccgccagg tagaggcggt caccgatcgg
     5341 ttcctgcagc cggcgccggt cttcgaggcc ggagccgggc gcgtggaacg aataggagcc
     5401 gcgcgcgtaa ggatcaacgg accaattcga cgtccggacc tcgaccggag agatgttgtc
     5461 gccgaacagt tgtcgagcga tgggcagcgc gctggtcatg agatccgcgg gcgaggacga
     5521 ttccaccgac cgcccgcggt caccggcatt gaaggccaac acgattgggc ccgcggcgcc
     5581 cggcagggtg aaccactgtg cccaaagacc gggttcggtg ccgatgaatt ggtaaaaagc
     5641 gttctctatg ttccacgttc gctgatcgaa gcggaagtag cttttggaca gcacgccgaa
     5701 ccccaacgcg tgcaacgcat gggagtgttc gtcgggtagg ggcggatcga aggtgatggc
     5761 accggatttc agcacgccga gtggaacggt gacgacggcg gcgggcccct cgaacgatcg
     5821 gttgccggct cgcacaatga ctgagccgtc gcgaagcgcg atcgcggtca ccggcgtatt
     5881 cagcacgatc tcaagaccgc cggcgagcag ccgcggcagg gcgtcgtagc cgctggtgac
     5941 cacgtcctgt tcgccgccgg cgtattcgcc cttgtcgaaa gtgttggcgg agagctgatt
     6001 cgcgtcggtg gcgtattcgt cctcgatttc ggtggtcaga tagaaagcca attgggcacg
     6061 atcggaatcg gaaagctccg cactgctcgc ggcggcgctg acggcggctc ccagactccc
     6121 gccgtcgacg tggtcgcgag cccgctcgac gaacttgcgc caggttttcg aatcgtagtc
     6181 cagcggcccc agccggggat caaccgcgag ctttgcccac ccgtagtagt cggtggggac
     6241 gagctgtgcc tgggcctggt gcgccagctc catcagcggg ttgtccgacg tgccgtggat
     6301 ccacgacgct cccatctcaa gcggtgcgcc ccagtcgcgg ttggtgcaca ctcggccgcc
     6361 gatgcgatcg cgggcttcga tgacgcgcac cggccggccc gcgtcggcga gggtgcgcgc
     6421 ggcggacagg ccggccatgc cggcgccgac aatgagcacc gacttgttat ccggcggtgg
     6481 tcggcgatcc gccgcgcacg cggccgccgt cccggcgccg accaagcttg ccgttgccgc
     6541 gaaaaagtcc cgacgggata gcggtgacac gggtgtcagc gtcccacatg actgtcggcg
     6601 caggcccgcg cctggccgca ccggttattg gacgatcgtc cgaaaatttg tctcgtgtgt
     6661 tcggccgatt ctgcgccgtt cgtcgttgcg ttcaggttaa actcgcgtgc atccacaatg
     6721 cggcagtgcg gccgtgttcc agggggagtg gcgatgagtg ctgggcaatc gactcgcaaa
     6781 accgagcggc ttcccgacgt gctaccacca agccgcgtcc tgaccgtgcg cgctgccgac
     6841 ggcaccccgt tgcacaccga ggtattcgga ccggccgacg gctatccgat cgtgttgacc
     6901 cacggcatca cctgcgcgat gcgggcgtgg gcacaccaga tcgcggacct ggccaccgat
     6961 taccgggtga tcgcgttcga ccatcgcggc catgggcgaa gcggcgtccc gcggcgtggc
     7021 gggtacagtc tcaaacgcct tgcatccgat ctggattcgg tgctcgaggc gacgttggcg
     7081 ccgcgcgagc gtgcggtgtt ggccggccac tcgatgggcg gcatcaccat cgccgcctgg
     7141 tcagcgcagt atcgccacca ggtccaccgg cgcgctgacg ccgtcgcgct gatcaacacc
     7201 accaccgggg atttggtgcg cgaggtgaaa ctgctgccgg tgccgcgccc gttgcgcggg
     7261 ctttcaacgg cgcgggtctt cgccgcgcgc agcctgatca acgtgttcgg cggttttccg
     7321 atcccacccg cggccgggat cccgagccgc tacctggtca cgatgctggc ggtcggaaag
     7381 gacgccgacc ccggcgccgc gcgactggtc cacgaattgt tcgtgcagac accgccttcg
     7441 gggcgcgggg gttgcgcaaa gatgctcgtc ggggcgttgg ggtcgcggta tctcgacttg
     7501 agcggtttga cggtgccgac gttggtcatc ggcagcgagc gcgatcggct gacgccgatc
     7561 agccagtcgc gcaggatcgc gcgcaccgcg cccaatgtcg tcggtctggt cgagttggcc
     7621 ggcgggcatt gctcgatgct ggaacagccc ggtgaggtga accggcagct gcgggcactt
     7681 gtggaatcgg ttacccatgg cgtgggggcc ggccgcatca gctcataaca gggcggcgac
     7741 ctcggcggct gcccgctgac cggacctgac ggcgccgtcg agaaagccgg tccattcgtc
     7801 cgccgtctcg gtgccggccc agtgaatcgg cccgaccggc ttgcgtaacc acggcccgaa
     7861 tcgtgtccac gacccaggtg gcaccgccgc cgtcggaccg cccggcgcga aatcttctgc
     7921 accccaacga tgatcgacat aatccagggg cttgagcgcc gcgtcgccga acagtctggc
     7981 aaagcagcgc agcgcgtcgc ggcggcgctg ctcggtggga agcgagtcga acgcccgggc
     8041 gtcgacgaaa cccatcagga tcccgggccc gtcggcgtgc gggctgacgt cgaaggtgat
     8101 gaacaccggg ccctggtcgg acagcgcctg gccggagaag ccgtcggcgc gccagaacgg
     8161 cgtctcgtag gccgcgtagg ccttgctgag ccggccctgc ggccaatgtt gggcgagttc
     8221 gacgtactcg tcgggcagcc ggggagcgaa atcgatcgac gcacgatggg ccggcgggat
     8281 cgctacgatg acgaacctgg ccccggcctg gccgacgtcg ctggtgaccg tcacgccggc
     8341 accgtggtgg tcgatgcggc gcaccggcgc gttcagcacc acacgggcac ccagttcggc
     8401 ggcggccgcc tcggcgatct gctgagtgcc gcccggaaaa cggtcctgct gggcgccgtc
     8461 ctcgacgtcc agcagccggt ccaggccgcc tgcggcgtgt gcgtagcggg aggcgtgcag
     8521 catcgacacg tcgtcgggct cacagcccca ggtcacccgg gccatgatgg ccagcaggtc
     8581 gcgcgaggag tcggaggcat gcaccgagcg caaccagtcg ccgagcgaca tgccgtccaa
     8641 ttcgcgggcg cggcgcgcat cccagggtgt tgccagcggg atactgcggg cgattctgtc
     8701 gaatcgccaa cgcaaccggc cgatgtcgag cagtccggtc agtgagagct tgggaatggt
     8761 gccgtggtag gtgcgcgccc agccgcgcca gtggatcagg ttcttgccgt cgtggtaggt
     8821 cggcgtcgtc ggaacctgga gctcggccgc cagcgcgaga accgcgtcct gggttgggcc
     8881 gacgaaagtg ccgccaagat cggcgggcaa ccccgcgaca ctgccggtga acgagcgccc
     8941 gcctacccgg tcccggccct cgaggaccag cgcctcatgt cctcgtcgcg tgagctcacg
     9001 cgccgcggcc agcccggcaa agccggcacc gatcaccaca acgtcggcgg tccgcggcgg
     9061 cgttgtcacg tcctgtagtc aaccgcattg cgacggtcgc gcaggtgctg gcgcatgcga
     9121 tatcaacttt ggacgtcatc gaccgcgtca gggcgcaacg gttagccagt cggtgaagcc
     9181 ggacgggtcg tggcggccca gcgcgccgtg ctcgaagaga ccccagccct cgaccggaac
     9241 cccgtcaccg tcgcggcaca cggcgcggcc cacgtgatcg atcacgccga agccggaccg
     9301 cgcaatgatc gccggatcgt tcatgtcgta ggtgagtcgt tggacgaact tctcgccctt
     9361 ccacatgccg tgcagccagt ccgagtcgcc gccgtagccg cccccgacgt ggatggggac
     9421 cggcagcttg gactccacgt cgaagtgcac cggggtgccg tcggtggtgg tcgcctcgat
     9481 cgtggcgccg gtcgggatgc gggtgccgga tcggtaatgg atcttgagcc gcggccagcc
     9541 cagctgctcg acacggccgt ggcgccagac ccgggtgcag tcgttgagcg agcggaaccc
     9601 gttgggctcc tcctggatga tcagcacgac ggcgaagtcg tcgaacgcca tcggcacgta
     9661 gagccaccac atgccctcga acggcgggtc ggcgggccgg ccggccggct cgggctcgcc
     9721 gatcggcctg atgccccacg agcggtcgcg gctgccgagc caggttccgg ggtcgaccgc
     9781 gatctcggcg tcgtcgacgg cgatgcggcc gctccaggtg ccgagctggg cgaatctctg
     9841 cgcgtccagc gtcactcggt tacccgagcg cattaggtgc ggctgctcct ggacgacgtc
     9901 gaagaggccc tcccaggtga gatcggctgc gatgccctcg gtttcgtcga ggaccagccg
     9961 caatttgcgc agcggttgga tcacctcgac ccggtagccg ttgacgtgct ggttgagccg
    10021 gtcggagtcg atggcgtccg aaaggtgcag cgcggtctgg gtgtccgcgc gccggatcag
    10081 cacgaacgcg tccttcaccc ctaggttggg gtagtagccg atgccgctga tgaaaaagat
    10141 gttgccggtg cggtcgtggg cgttgaagta ggagcggtcg tagaagttgc ggtccgatgg
    10201 acccggccac gcgatcggct gggggagctg gtgtaccggg aactcgtcga gcgggccaag
    10261 tagttgcggc attagcgctc ctctccgatg agacgtttca tcagtccggc gtggtagaac
    10321 aacgactcga catcgtcggg cttctcgacc tccccgaagt gcacccgccg cgcgccggtg
    10381 cgcatgaaca cgcaggccca catcacgccc gagtagacgt aaaaccagtt caggtcgccc
    10441 acttcgacac cggtgagccg ccggtaggtg gcccggacgt cgtcctctct catcacctcg
    10501 ggcagccccg gcagtgtcgc caggccggcg agctcttgaa aaaccatgtg cgcgtagatc
    10561 atccacgcga cgtccagttc gcgtgggccc agcgtcacca tttcccagtc cagcaccgcc
    10621 accggccgaa aatctcggta caacacgttg cccacccgtg cgtcgcccca caacaacacg
    10681 ggctcgtggg cggccacctc ttgcggccag ttggcttcca gccatgcgaa agtccgctcc
    10741 agcagcggtg atcggccgat gtcgggcacc gcgaagtcgt accaggaacg gacccagttg
    10801 aagtgcccgc gcaacgccgt gtcgcctcgc cgtccttcgg acaggaaggc gaacgtcttc
    10861 tccgcgttgg gaatcgaatg cagcgatgcc agcaccccca ccgtggcgtc ctgcagttcg
    10921 cgctgacgtt cggcgggagc gtcggcgaac cagttgccgc cgaaggtgta gggcatgacg
    10981 tcgggcggta ccacgccgtc gacgtagtcc atcagaaaga agggtgtgcc cagcacgtcg
    11041 ccggtgttct cgagccaccg aacgcgcggg accgggacgt cggtgagttc gccgaccagc
    11101 cggatcacct cgaattggtg gtctaagcga taggtgggga agacccgcac gtcctcggcg
    11161 gtgggggcca cccgggccac cagcttttcc tcgatcgact gcccgtcctg ctgccggcgg
    11221 gcggtcagga ttatggtttc cgacgacatg cccgtcgagt ccacgccgct ttccacggtc
    11281 acctcgggta gcgccccgcc gggaaggacg gttgacagcc agtgcgacat cacggcaggc
    11341 aaggttgtga cgtcgcggct ggagcgctgc aggtgttcca cgtcgtggat cgcgggttcg
    11401 gtggccacgg gtgcctccat tcgttgagac ttcttcgcgg caattacgat acggtaggta
    11461 gcgttatgaa agcagacccg tccacccttg acaaggcccc cggcgccggc cgtccccggg
    11521 atcggcgtat cgattcggca atcctggcgg cgaccgcgga actgcttgtg caaatcgggt
    11581 attcgaacct cagcctggcc gcggttgccg agcgcgccgg caccacgaag tcagcgctgt
    11641 accgccggtg gtcgagcaag gccgaactgg tgcacgaggc cgccttcccg gtggccccga
    11701 ccgctctgga ggcgccggcc ggcgacatcg ccgccgacat ccgcatgatg gtcgaggcca
    11761 cccgcgacgt gttcaccacc ccggtggtgc gcgccgcgtt gcccggtctg gtcgccgaca
    11821 tgacggccga tcccgccctc aacgagcgag tgatgtcgcg tttcaccgac ctgttcaccg
    11881 cggtgcgggt ccggctgcgc gaggctgtcg accggggcga ggcgcatccc gatgtggacc
    11941 cggaccgcct gatcgaattg atcgggggtg cgacgatgct gcggatgctg ctgcgcccgg
    12001 aggaagaact cgatgacaca tgggtggagc agaccaccgc catcgtggtg cacggggtga
    12061 cgggatgacg ggcgcgcggt ggcgatccgt gccgatccca ccgccgcggc cgtgcacgct
    12121 gcgttaagcc ggcttggccg ccttcacgcc gttgataa
//
LOCUS       DASSGQ010000129         7862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_69620, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000129 DASSGQ010000000
VERSION     DASSGQ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69620"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFI55_06080"
     CDS             <1..186
                     /locus_tag="VFI55_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073636.1"
                     /translation="IGTGLTNMTDRLSAIGGDLVIDTAPGRGTRVTAVVTTSVPPGSP
                     ELPGNGEPRVGLTHSRR"
     gene            complement(210..1400)
                     /locus_tag="VFI55_06085"
     CDS             complement(210..1400)
                     /locus_tag="VFI55_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HET7073637.1"
                     /translation="MTVVTGAPANGGSCVAHHEGRVVFVRYALPGERVRVRVTADRGS
                     YWHAEVVEVIEPSADRVESVCPIAGVDGAGCCDLAFAAPAAARVLKAQVVANQLQRLG
                     GYEWRVPDTGAEPLSESCFTGWRTRVRLDVGADCRPGFHRYHSDELVTDLRCAQLPAG
                     MLDGLAEACAEAGISPGAELHVAVDDDGTRHVVRTLRQGKRTATKVVEGGYEAVQRVG
                     ARSWLVPVTAFWQAHRDAVRVYSDLVAEWAQPGAGMTVWDLYGGTGVFAAVLADAVGE
                     SGRVLTVDTSRGATRAARTALVDLPQIDVITDSVRRALTAQRAGADVAVLDPPRAGAG
                     REVIDLLAAAGVSRVVHIGCEAASFARDIGLYQGHGYVVEKIKVFDAFPLTHHTECVA
                     LLTR"
     gene            complement(1469..3463)
                     /locus_tag="VFI55_06090"
     CDS             complement(1469..3463)
                     /locus_tag="VFI55_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET7073638.1"
                     /translation="MSKLSTAARRLLIGQPFRSDRLSHTLLPKRIALPVFASDALSSV
                     AYAPEEIFLMLSVGGLSAYALSPWIGLAVAAVMLVVVASYRQNVHAYPSGGGDYEVVT
                     TNLGGTAGLVVASALMVDYVLTVAVSTASAMSNIGSAIPVVAQNKVWFCVAAILLVMA
                     MNLRGVRESGLAFAIPTYAFIVGVACMIVWGFIRIFVLDNPLRAESAGFQMHSEHGQI
                     VGFALAFLVARSFSSGCAALTGVEAISNGVPAFERPKSRNAATTLLMLGGIAVTLLMG
                     IIVLSKAIGVQMVEDPATQLIGAPPKYYQKTLVTQLAETVFGSFHIGFLLITAVTALI
                     LVLAANTAFNGFPVLGSILAQHSYLPRQLHTRGDRLAFSNGILFLSAAALAAIIAFRA
                     ELSALIQLYIVGVFISFTLSQIGMVRHWTRLLRSETDPRVRRKMMRSRIVNTVGLLST
                     GAVLVVVLVTKFLAGAWIALFAMSALFIIMKLIHKHYNTVNRELAEQEAAQHDVVLPS
                     RNHALVLVSKLHLPTLRALAYARATRPDALEALTVSVDDAETRDLVRKWQDSDISVPL
                     KVIASPYREITRPVLDYVKRVSKESPRTVVTVFIPEYVVGHWWEQVLHNQSALRLKGR
                     LLFMPNVMVTSVPWQLSSSERLKAFQPHAAPGDARRGIFD"
     gene            3598..4281
                     /locus_tag="VFI55_06095"
     CDS             3598..4281
                     /locus_tag="VFI55_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET7073639.1"
                     /translation="MRVVVMGCGRVGSSLSGGLSRIGHDVAVIDRDSTAFNRLSPEFS
                     GERVLGQGFDRDVLLRAGIEEADAFAAVSSGDNSNIISARLARETFGVRRVVARIYDA
                     KRAEVYERLGIPTIATVPWTTDRLLNVLTRESETAKWRDPTGTVAVAEVDLHEDWVGH
                     RATDLEQATGARVAFMIRFGTGILPEPKTVIQAGDQVYIAAISGRAAEAVAIAALPPS
                     DDLETGPRK"
     gene            4411..5088
                     /locus_tag="VFI55_06100"
     CDS             4411..5088
                     /locus_tag="VFI55_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET7073640.1"
                     /translation="MKVAIAGAGAVGRSVTRELLENGHDVTLIERNPDHVDVDSIPAA
                     HWRLGDACELSLLQSVHLEDFDVVVAATGDDKANVVLSLLAKTEFAIPRVVARVNDPR
                     NEWLFTDAWGVDVAVSTPRMLASLIEEAFTVGDLVRLMEFRRGQANLVEITLPDDTPW
                     GGRPVRRLQLPRDAALVTILRGPRVIVPEADEPLEGGDELLFVAAAEVEEDLRKLLLP
                     EGPPLAD"
     gene            complement(5085..5750)
                     /locus_tag="VFI55_06105"
     CDS             complement(5085..5750)
                     /locus_tag="VFI55_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HET7073641.1"
                     /translation="MTTNRMSPERLLEQVGGVSGIIYSSLPVVVFVVVSSVSGLLPAV
                     GAAVGVAALVLLWRLIRRGSVQPALSGFMGVGICALIAYLLGQSKGYFLLGIWMSLLW
                     AVIFAASVLIRRPVVGYAWSWASGRDGAWRAVPRAVRAFDIATLCWVLVFGARFVVQR
                     LLYDADQTGWLGVARIGMGWPLTLLAALATYAAIKAAQRAVSTAADGAADAGGIDADL
                     GRR"
     gene            complement(5869..6237)
                     /locus_tag="VFI55_06110"
     CDS             complement(5869..6237)
                     /locus_tag="VFI55_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HET7073642.1"
                     /translation="MGAQGYLRRLTRRLTEDPEQRDSAELSDEIASTGAQRAIDCERG
                     QEVTMVGTLRSVECNGKGCAGGVRAELFDGSDTVTLVWLGQRRIPGIDSGRKLRVRGR
                     LGKLENGTKAIYNPHYEIQR"
     gene            6373..7071
                     /locus_tag="VFI55_06115"
     CDS             6373..7071
                     /locus_tag="VFI55_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7073643.1"
                     /translation="MAVDLRGVTTVLLPGTGSDDEYVRRTFFDPLREVGALLIAPAPR
                     PDRLIDGYLSALDEAARGGPIGVGGVSIGAAVAAAWALAHPGQAIAVLAALPAWAGAP
                     GSAPAALAARYSASQLRADGLAATTAQMRASSPPWLGDELARSWRAQWPQLPDAMQEA
                     AAYVAPSLADLSRLAAPLAVAAAVDDPIHPLQVAVDWVAATPRAALRTVTLDQLGTDP
                     TALGVACLAALADL"
     gene            complement(7095..7862)
                     /locus_tag="VFI55_06120"
     CDS             complement(7095..7862)
                     /locus_tag="VFI55_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3710 domain-containing protein"
                     /protein_id="HET7073644.1"
                     /translation="MAFGRRSDKDDSEGTSSEQEPQDELEAPVADEGDFDDVDEVEGP
                     FDIDDFDDPAVAQLARLDLGSVLIPMPEAGQLQVELTETGVPSAVWVVTPNGRFTIAA
                     YAAPKTGGLWREVAGELAESLRKDSAKVSIKDGPWGREVVGTASGVVRFIGIDGYRWM
                     IRCVINGSHETIEALDQEARAALADTVVRRGDTPLPVRTPLAVQLPEPMAEQLRAAAA
                     AQQASQDAGAEQPPTEPAARRSVEGSAMQQLRTTTGG"
BASE COUNT         1356 a         2720 c         2593 g         1193 t
ORIGIN      
        1 atcggcaccg ggctaaccaa tatgaccgac cgcctatcag ccatcggcgg cgacctcgtc
       61 atcgacaccg cccccggacg cggcacccgc gtcaccgccg tcgtcacgac ctcggtgcca
      121 ccaggcagcc cggaactgcc cggaaatggt gaaccacgtg ttggtctgac gcactcccgg
      181 cgttgagctt ggaggacaat tgccctgccc taacgggtca gcagcgcaac gcattcggtg
      241 tgatgggtca acgggaacgc atcgaacacc ttgatcttct cgacgacgta accatgacct
      301 tgatataggc cgatatcgcg cgcgaaagat gctgcctcgc aaccgatgtg aacgacgcgc
      361 gacaccccgg cggcggccag tagatcgatc acctcgcgcc cggcacccgc ccgcggcgga
      421 tccagcacgg cgacatcggc accggcgcgt tgcgccgtca gcgcgcggcg gaccgaatcg
      481 gtgatgacgt ctatctgcgg cagatcgacc agtgcggtcc gtgcggcccg cgttgccccg
      541 cgtgaagtgt cgaccgtcaa cacccgcccc gactccccca ccgcgtcggc gagcacggcc
      601 gcgaaaacgc ccgtcccgcc gtacagatcc cacacggtca taccggcgcc gggctgcgcc
      661 cactcggcga ccaggtcgct gtagacgcgc accgcgtcgc ggtgcgcctg ccagaacgct
      721 gtgaccggaa ccagccagct gcgggccccc acccgctgta ccgcctcgta gccgccttcc
      781 accaccttgg tcgcagtcct cttgccctgc cgcagagtgc gcaccacgtg gcgcgtgccg
      841 tcgtcgtcga cggccacgtg caactcggcg cccggcgata tgccggcctc ggcacaggcc
      901 tcggcgaggc cgtccagcat gccggccggc agttgcgcgc agcgcagatc ggtcaccagc
      961 tcgtcgctgt ggtagcggtg aaagccgggg cggcagtccg cgcccacgtc cagacgaacc
     1021 cgggtgcgcc aacccgtgaa gcaggactcc gacaggggtt cggcccccgt gtccgggacc
     1081 ctccattcgt agccgcccag ccgttgcagc tgatttgcca cgacttgcgc cttgagcacc
     1141 cgggccgccg ccggcgccgc gaacgccaga tcgcagcacc cggctccgtc cacgccggcg
     1201 atggggcaca ccgattcgac ccgatcggcc gacggctcga tcacctcgac aacctctgca
     1261 tgccagtagg agcctcggtc cgcggtgacg cgcacccgca ctcgctcgcc gggcagtgcg
     1321 taacggacga acacgacgcg gccttcatgg tgcgccacgc aactaccgcc gttggcgggg
     1381 gcgccggtca ccaccgtcag ctccgcaccg gccgacggcg tcggcgcctc cggcctcgtc
     1441 tcacccggta cgggcgtggg ctcggcgctc aatcgaagat tccgcgacgg gcgtcgccgg
     1501 gcgcggcgtg gggctggaac gccttgagtc gctcggacga actcaattgc cagggaacgg
     1561 acgtcaccat cacgttgggc atgaacagca accggccctt gagccgcaag gcgctttggt
     1621 tgtgcagtac ctgctcccac cagtggccca ccacgtactc ggggatgaac accgtcacca
     1681 cggtgcgcgg cgactccttg ctgacccgtt tgacgtaatc gagtaccgga cgggtgattt
     1741 cacggtaggg cgacgcgatg accttcaacg gcacgctgat gtcgctgtcc tgccatttgc
     1801 gcaccagatc gcgggtttcc gcgtcgtcga cgctgaccgt gagggcctca agtgcatcgg
     1861 ggcgggtcgc gcgggcgtag gccagcgcac gcagggtcgg cagatgcagt ttcgacacca
     1921 gcaccagggc gtgattgcgg ctgggcaaca ccacgtcgtg ttgagcggcc tcttgttccg
     1981 ccaattcccg gttgacggtg ttgtagtgct tgtggatcag cttcatgatg atgaacagcg
     2041 cactcatcgc gaacagcgcg atccacgccc ccgcaaggaa cttggtgacc agcacgacga
     2101 caagaaccgc gccggtggaa agcaacccga ccgtgttgac gatccgcgat cgcatcatct
     2161 tgcgccgcac ccgcggatcg gtctcggagc gcagcagccg agtccagtgc cggaccatgc
     2221 cgatctgact cagcgtgaag gaaatgaaca cgccgacgat gtagagctgg atcagcgcgg
     2281 acagttcggc ccggaaagcg atgatcgccg ccagcgcggc cgccgacagg aacaggattc
     2341 cgttcgagaa cgccagccgg tccccgcggg tgtgcagctg ccggggcagg taactgtgct
     2401 gggccaggat cgagccgaga accgggaacc cgttgaacgc ggtgttggcc gccagcacca
     2461 ggatgagcgc ggtcacggcg gtgatcagca ggaacccgat gtggaagctg ccgaacaccg
     2521 tctccgccag ctgcgtcacc agtgtcttct ggtagtactt cggaggcgcg ccaatcagtt
     2581 gggtcgccgg gtcttcgacc atttgaacgc cgatcgcctt ggacagcacg atgatgccca
     2641 tcagcagcgt caccgcgata ccgccgagca tcagcagggt cgtcgctgcg ttgcgcgact
     2701 tgggtctctc aaacgccggc accccgttac tgatcgcctc cacaccggtc agcgccgcgc
     2761 acccggacga gaacgatcgc gctaccagga acgccagcgc gaatccgact atctggccgt
     2821 gctcggagtg catctggaaa ccggcggatt cggctcgtaa tgggttgtcc agcacaaaga
     2881 tccggatgaa cccccaaacg atcatgcacg ccacgccgac gatgaaggcg taggtgggga
     2941 tcgcgaaggc caacccggat tcgcgcaccc cgcgcaggtt catggccatc accagcagaa
     3001 tggcagccac gcagaaccac accttgtttt gggcaacgac agggatcgcc gacccgatgt
     3061 tggacatcgc cgaggccgtc gaaacggcga ccgtgagaac gtaatccacc atcagggcac
     3121 tcgcgacgac gaggccggcg gtgccgccca gattcgtggt gaccacctcg tagtcgccgc
     3181 cccccgaagg gtaggcgtgc acgttctgtc ggtagctggc caccaccacc aacatcaccg
     3241 cggccaccgc caacccgatc cacggcgaca gcgcgtaggc ggacagaccc cccaccgaca
     3301 gcatgaggaa gatctcctcg ggcgcgtagg ccaccgagga cagcgcatcg gaggcgaaca
     3361 ccggcaaggc gatccgcttg ggtagcaggg tgtgactcaa ccgatcgctg cgaaacggct
     3421 gaccgatcag caatctgcgc gctgcggttg aaagtttgga cacgagagcc aagggtaagc
     3481 gtagccgggc ttggtagcgt ttcgtacgcg agaatgttct gcacggactg aaatcggcac
     3541 gccaaccgag ggtgccgaat agtagacaga agcaggaccc agcggagagg acctcaggtg
     3601 cgggtggttg tgatgggttg cggccgggtc ggctcgtcgt tgtccggtgg cctgtcccgc
     3661 atcggccatg acgtggcggt gatcgaccgc gacagcaccg ccttcaatcg gctcagcccg
     3721 gagttctccg gcgagcgggt gctgggtcag ggattcgacc gagacgtact actgcgggcc
     3781 ggaatcgagg aagccgacgc gttcgccgcg gtgtcatccg gtgacaactc caacatcatt
     3841 tcggcgcggc tggcccggga gacctttgga gtgcggcgcg tcgtcgcccg gatttacgac
     3901 gccaagcgcg ccgaggtcta cgaacgcctc ggcattccga cgattgccac ggtgccctgg
     3961 accaccgatc gcctgctcaa cgtgctgacc cgggagagcg agaccgccaa gtggcgcgat
     4021 ccgaccggta ccgtcgccgt ggccgaggtc gatctgcacg aagactgggt aggccaccgg
     4081 gccaccgatc tggagcaggc aaccggtgcc cgcgtggcgt ttatgatccg gttcgggacc
     4141 ggtatcttgc ccgaaccgaa gactgtcatc caggccggcg atcaggtcta catcgccgcg
     4201 atttccggcc gcgccgccga ggcggtagcc atcgccgccc tgccgcccag cgacgatctc
     4261 gagacgggac cacgcaaatg aaacgcaacg acgaagacgc cggcttgcgt aattttcgag
     4321 gcggggttga ggagcggcgc acatgaagcg cgacgacgac gaagacgccg gctcgcgtaa
     4381 ttttcgaggc ggggttgagg agcggcgcac atgaaggtag ctatagccgg ggccggcgcg
     4441 gtcggtcgct cggtcactcg cgagctcctc gagaacgggc acgacgtgac cctgatcgag
     4501 cgcaaccccg accacgtcga cgtcgactcc atcccggccg cgcactggcg tctgggcgat
     4561 gcctgcgaac tgagtctgct gcagtcggtg cacctcgagg atttcgacgt cgtggtcgcc
     4621 gcgacgggcg atgacaaggc caacgtggtg ctcagtctgc tggccaaaac cgagttcgcg
     4681 ataccccggg tggtggcccg ggtcaacgac ccccgcaatg aatggctgtt caccgacgcc
     4741 tggggcgtgg acgtggccgt gtccacgccg cgcatgctgg cctcgctcat cgaggaggca
     4801 ttcaccgtcg gcgatctcgt gcggctcatg gagttccggc gtggccaggc caacctggtc
     4861 gagatcaccc tgcccgacga cacgccgtgg ggcggccggc ccgtgcgcag gctgcagctg
     4921 ccgcgggatg ccgcactggt aacgatcctg cgcggcccgc gagtcatcgt gccggaggcc
     4981 gacgaaccgc tggaaggcgg cgacgagctg ctcttcgtcg cggccgctga ggtcgaggag
     5041 gacctgcgca agctgctgct gccggaaggc ccgccgctcg ccgactaacg gcgacccagg
     5101 tcggcgtcga tcccgccagc gtcggcagcg ccgtcggcgg cggttgacac cgcgcgctgg
     5161 gcggccttga tggccgcgta cgtcgccaga gcggccagca gggtcagcgg ccaccccatc
     5221 ccgatgcgcg ccacgccgag ccagccggtc tggtcggcgt cgtagaggag ccgctggacc
     5281 acgaatcgcg cgccgaaaac cagcacccag cacagcgtcg cgatgtcgaa cgctcggacg
     5341 gccctgggca cggcgcgcca ggcaccgtca cgcccgctgg cccagctcca ggcgtagccg
     5401 acgacggggc ggcggatcag caccgaagcc gcgaaaatca ccgcccacaa caacgacatc
     5461 cagatgccga gcaggaagta gcccttggac tgccccagca ggtaggcgat cagcgcgcag
     5521 atgccgaccc ccatgaaacc ggacaacgcc ggctgcacgg atccccgccg gatcagccgc
     5581 cacagcagga ccagggcggc cacgcccacc gcggccccga cggcgggcag caacccggaa
     5641 acgctggaaa ccaccacgaa gaccaccact ggcagcgacg agtagatgat gccgctgaca
     5701 ccgcctacct gttccagcag acgctcgggg ctcatgcggt tagtggtcac ggcactccat
     5761 ggatccgaaa gactccgatg gatcgaaaag acgacgatgg atcgaaagac gacgatggac
     5821 ccgaaagccg gattggtcgt gggccagccg ttagtacgcc agctgcggtc accgttgaat
     5881 ttcgtagtgc gggttgtaga tggccttcgt cccgttctcc agcttgccca gccgaccgcg
     5941 cacgcgcagc ttgcggcccg agtcgatgcc ggggatgcgg cgttggccca gccacaccaa
     6001 cgtcacggtg tcgctgccgt cgaacagttc ggcccggacg ccgcccgcgc agcccttgcc
     6061 gttacattcc acgcttcgca aggtgccgac cattgtgact tcctggccac gctcgcaatc
     6121 gatggcacgc tgtgcgcctg tgctggcgat ctcgtcggac aactccgcgg agtcgcgttg
     6181 ttccggatcc tccgtcaacc gacgggtgag ccggcgcaga taaccttggg cccccatggc
     6241 cactcctgac aatgctgata atcctcggcg ggtgcactat gccaacggac accgtagacc
     6301 tgttggttcc ccggcgccaa ccgaacgaaa cgcgaagttg gagcgtgcat gccggccggg
     6361 caatatcgac gtgtggctgt cgatctacgc ggtgtcacga cggtgttatt gcccgggaca
     6421 ggctccgacg acgagtacgt gcgccggacg tttttcgacc ccctgcgtga ggtcggcgcg
     6481 ctgctgatcg ccccggcgcc ccggccggac cggttgatcg acggctatct gtccgccctg
     6541 gacgaagccg cacgaggagg tccgatcggc gtaggcggtg tctcgatcgg tgccgcggtg
     6601 gccgccgcgt gggcactggc gcatccgggc caggcgatcg cggtcctggc cgcgctgccg
     6661 gcctgggccg gagccccggg atcggcaccg gctgctctcg cggcacggta ttcggcgtcc
     6721 cagctgcgcg ccgacggttt ggcggcgacg acggcacaga tgcgggcatc cagcccaccg
     6781 tggctgggcg acgagctggc gcgctcgtgg cgcgctcagt ggccgcagtt gcccgacgcc
     6841 atgcaggaag cggcggctta cgtcgcgccc agccttgccg acctcagccg gctggcggca
     6901 ccgctggcgg tcgccgcggc ggtcgacgat cccatccatc cgctgcaggt agccgtcgac
     6961 tgggtcgccg cgacgccgcg cgcagcattg cggacggtga ctttggatca gctgggcacg
     7021 gaccccaccg cgctgggcgt ggcctgcctg gccgcactcg ccgaccttta gggcgcgccc
     7081 aactgggtgc cgcctcagcc gccggtcgtg gtgcgcagct gctgcatggc cgatccctcg
     7141 acgctgcggc gtgcggccgg ctcggtgggc ggctgctcgg ccccagcgtc ctgcgaggcc
     7201 tgctgcgcgg ccgcggctgc ccgcagctgc tccgccatcg gctcgggcag ttgcacggcc
     7261 aacggcgtac gtaccgggag cggtgtgtca ccgcggcgaa ccacggtgtc cgccaacgcg
     7321 gcacgcgcct cctggtccaa cgcctcgatg gtctcgtgcg agccgttgat gacacaccgg
     7381 atcatccagc ggtacccatc gatcccgatg aagcgcacca cgccggaggc ggtgccgacc
     7441 acctctcgac cccacgggcc gtccttgatg ctgaccttgg ccgagtcctt gcgcagcgac
     7501 tcggcgagct cgcctgctac ttcacgccag aggccgcccg tcttgggtgc tgcgtaggcg
     7561 gcgatggtga aacgaccgtt gggtgtgaca acccacaccg cgctcggcac accggtctcg
     7621 gtcagctcga cctgcagctg gccggcctcc ggcatgggga tcagcaccga gcccaggtcg
     7681 agcctggcca gctgggcgac cgccgggtca tcgaagtcgt cgatgtcgaa cgggccctcg
     7741 acctcgtcga cgtcgtcgaa gtcgccctcg tcggcgacag gcgcctcgag ctcatcctgc
     7801 ggttcctgct cgctggaggt gccttcgctg tcgtctttgt ccgagcgtct accgaatgcc
     7861 ac
//
LOCUS       DASSGQ010000130         8109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_799739,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000130 DASSGQ010000000
VERSION     DASSGQ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8109
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799739"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..878)
                     /gene="rnc"
                     /locus_tag="VFI55_06125"
     CDS             complement(165..878)
                     /gene="rnc"
                     /locus_tag="VFI55_06125"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET7073645.1"
                     /translation="MTSRQTLLEALGVDLPEELLSLALTHRSYAYEHGGLPTNERLEF
                     LGDAVLGLTITDELYHRHPDRSEGDLAKLRASVVNTQALADVARNLSDGGLGVHLLLG
                     RGEANTGGGDKSSILADGMESLLGAIYLQHGIETARAVILRLFGPLLDAAPTLGAGLD
                     WKTSLQELTASRTMGSPSYMVTSTGPDHDKEFTAVVVVMDTEYGSGVGRSKKEAEQKA
                     AAAAWKALEALDTAGKTSV"
     gene            complement(875..1483)
                     /locus_tag="VFI55_06130"
     CDS             complement(875..1483)
                     /locus_tag="VFI55_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET7073646.1"
                     /translation="MTRQHSTSWPRGAAPRPAEAMTIDITRLGRRPGAMVTLRDTVPS
                     PSRIGLDMIAIQRGAPLELDLRVESVSEGVLVTGTVAGPTVGECVRCLTAVNGRVQVG
                     LTELFAYPGSTTEVTTEEDEVGHIVDDKIDLEQSVIDAVGLELPFSPVCSPDCPGLCP
                     ECGVALGSEPGHDHDRVDPRWAKLTQMLAPEPPDPDTSRGER"
     gene            complement(1554..2291)
                     /gene="sepIVA"
                     /locus_tag="VFI55_06135"
     CDS             complement(1554..2291)
                     /gene="sepIVA"
                     /locus_tag="VFI55_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepIVA"
                     /protein_id="HET7073647.1"
                     /translation="MYRVFEALDELGAIVEEARGVPMTSGCVVPRGDVLELIDDIKDA
                     IPGELDDAQDVLDARDSMLHDAKTHAESMVSTATTESESMLNHARAEADRLLSDAKAQ
                     ADRMVSEARQHSERMVGEAREESIRIATASKREYEASVGRAQAECDRLMENGNISYEK
                     AVQEGIKEQQRLVSQNEVVQAANAESTRLIDTAHAESDRLRGECDIYVDNKLAEFEEF
                     LNGTLRSVGRGRHQLRTAAGTHDYATR"
     gene            2579..3325
                     /locus_tag="VFI55_06140"
     CDS             2579..3325
                     /locus_tag="VFI55_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7073648.1"
                     /translation="MSGPTQPSKDDARPAVPTLYIFPHAGGSATFYVPLAKAFSGGVK
                     TIAVQYPGRRDRQAMTQLTSIPALAEEIFTMMAPSAGTDGHVAFFGHSMGGLLAFEVA
                     LRFQSAGHPISALFVSSCAAPGHIRYKELQGSDRDILNLVAAATNTNPELLATDEFAA
                     TVLPTLQSVRAIAEYTRPAELTVSCPIYGFLGDNDIMVARENMEPWRDRTSADFSVRM
                     FAGDHFYFNRHLPELVKEIEENALRPCPRS"
     gene            3978..5729
                     /gene="fadD26"
                     /locus_tag="VFI55_06145"
                     /gene_synonym="faaL26"
     CDS             3978..5729
                     /gene="fadD26"
                     /locus_tag="VFI55_06145"
                     /gene_synonym="faaL26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--AMP ligase FAAL26/FadD26"
                     /protein_id="HET7073649.1"
                     /translation="MPLLPSSIPALLQARAEQQPDAPAFTYVDYGQDPGGFAESLTWS
                     QVYRRARTLAEELALWGSPGDRVAILAPQGLEYVVAFLGALQAGFVAVPLSTPQYGIH
                     DDRVSSVLRDSQPVAILTTSAVVTDVAKYATGQDGRPAPLVIEIDLLDLDSPHEMSRT
                     AEFFSGAAYLQYTSGSTRAPAGVVVSHKNVIANVTQSLYGYFGDPAKVPTGSVVSWLP
                     LFHDMGLILGICAPMVAGRSAVLLSPMAFLRRPACWMQLLATSGECFSAAPNFAFELT
                     VRRTSDDDMAGLDLGKVVGIVSGSERIHVATVKRFTERFAPYNLRPAAVRPSYGLAEA
                     TLYVAAPEAGTAPKTVRFDYEALTAGEARTCGVEGTIGTELVSYGSPEPSSVRIVNPE
                     NMIESPPGVVGEIWVHGDHVAMGYWRKPEQTARTFNAALVDPAPGTPKGPWLRTGDVG
                     VMSDGELFIMGRIKDLVIVDGRNHYPDDIEATIREITGGRVAAISVPDDLTEQLVAII
                     ELKRRGASAEEAMLKLRSVKREVTSAISKSHSLRVADLVLVSPGSIPITTSGKIRRSA
                     CVERYRSDGFKRLDVSA"
     gene            5726..>8109
                     /locus_tag="VFI55_06150"
     CDS             5726..>8109
                     /locus_tag="VFI55_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET7073650.1"
                     /translation="MTSGINNEADLRHWLVDYLVTNIGCTPDEVDPDLSLADLGVSSR
                     DAVVLSGELTEVLGRTVSPIDFWEHPTINALAAYLTAPQPGPEWQAALERPARSLLEE
                     PIAVIGMGCRFPGGIRGPEALWQFLIDRRSSIGRVPDERWELFDDGSPEVKALLARTT
                     RWGSFLPDIDAFDAEFFEISPSEADKMDPQQRLLLEVAWEALEHAGIPPNSLRRSQTG
                     VFAGSCLSEYGAIASTDLPQVDGWSNTGGAMSIIANRLSYFLDLRGPSVAIDTACSSS
                     LVAIHLACQSLRTQDSHLAIAAGVNLLLSPAVFRGFDQVGALSPTGRCRAFDAAADGF
                     VRGEGAGVVVLKRLTDAQRDGDRVLAVICGSAVNQDGRSNGLMAPNPAAQMAVLRAAY
                     ANSGMQPHEVDYVEAHGTGTLLGDPIEARALGTVLGRGRAEDAPLLIGAVKTNLGHTE
                     AAAGIAGFIKTVMAVQRGQIPPNQRFESPNPHIPFADLRMKVVDTQTEWPDTGHPRRA
                     GVSSFGFGGTNAHVVIEQGQEVATQPRPGSDPGVSTLVVSGKTPRRVAATAAVLADWM
                     EGPGAQVPLADVAHTVNHHRARQAKFGTVVARERVHAVAGLRALAAGRRAPGVVCPQE
                     STPGPGMVFVYSGRGSQWAGMGRQLLADEPAFAAALGELEPVFVEQAGFSLRDVVANG
                     EELVGIEQIQLGLIGMQLALTGLWRSYGVHPDLVIGHSMGEVAAAVVAGALTPAEGLR
                     VTATRSRLMAPLSGHGGMALLELDAAATETLIAGYPQVTLGIYNSPRQTVIAGPT"
BASE COUNT         1358 a         2693 c         2621 g         1437 t
ORIGIN      
        1 aagcgcggcg aagccggacg cggcgggtca tcaccaccga ccagtgatga tcgcaagcgc
       61 ggctgagccg ggcgttgggg gtaccgcccg cttgcggggg acgggtcatc accatcgacc
      121 accgatgatc gcaagcgcgg cgaagccggg cgtagccggt cgatctagac ggacgtcttc
      181 cccgcggtgt cgagcgcttc cagcgccttc cacgcggcgg cggcggcttt ctgctcggct
      241 tctttcttag agcggcccac gccagacccg tattcggtgt ccatcaccac gaccacggcg
      301 gtgaattcct tgtcgtggtc cggaccggtg gaggtgacca tgtaggacgg cgagcccatc
      361 gtgcgcgaag ccgtcagctc ctgcaggctg gtcttccagt ccagcccggc acccagcgtg
      421 ggcgcggcgt cgagcagcgg gccgaacaac cgcaggatca ccgcgcgcgc tgtctcgatt
      481 ccgtgctgca ggtaaatcgc gccgagcagc gattccatgc cgtcggccag aatgctggac
      541 ttgtcgcctc cgccggtgtt cgcctcgccc cggcccagca gcaagtggac gccaagaccc
      601 ccgtcggaca ggttgcgcgc gacgtcggcc agcgcctgag tgttgaccac gctggcccgc
      661 agcttggcca gatcgccctc cgaacggtcg ggatgacggt ggtacagctc gtcggtaatg
      721 gtcaggccca ggacggcatc gccgagaaac tccagacgtt cgttggtcgg tagcccgccg
      781 tgctcgtagg catagctgcg gtgtgtcaac gccagcgaca gcagctcttc gggcaggtcg
      841 acaccgagcg cttcaagcag ggtttgccgc gaggtcaccg ttcgccccgt gaggtatccg
      901 gatcgggcgg ttcgggtgcc aacatctggg tcagcttggc ccaccgcggg tcaacgcggt
      961 cgtggtcatg gccgggctcg ctgccgagcg caacgccgca ttcgggacac aatcccggac
     1021 aatccgggct gcacaccggc gagaacggca gctccagccc gacggcgtcg atgacggatt
     1081 gctcgaggtc gattttgtcg tccacgatgt gaccgacctc gtcttcctcg gtggtcacct
     1141 cggtggtgct gcccgggtat gcgaacagtt cggtcaaccc aacctgaacg cggccgttga
     1201 cggcggtcag gcaacggaca cactcgccga cggtgggccc agccaccgtt cccgtgacca
     1261 atacgccttc cgacaccgac tcgacccgca agtccagttc caacggagcg ccccgctgga
     1321 tcgcgatcat gtccagtccg atgcgcgatg ggctgggcac agtgtcacgc agggtgacca
     1381 tcgcgcccgg acgtcgcccg aggcgggtga tgtcgatcgt catcgcctcg gccggccgtg
     1441 gcgctgcgcc gcggggccat gatgtgctgt gctgccgcgt catagatgaa atcctacggc
     1501 gcgagccgcg tcgcgccagc gcctgaccgc ggagttgacg ggcgccgata cagctagcgc
     1561 gtcgcgtagt cgtgggtacc ggcggccgtc ctgagttggt ggcgaccgcg ccccaccgac
     1621 cgcagggtgc cgttgaggaa ctcctcgaat tcggcgagct tgttgtcgac gtagatgtcg
     1681 cactcacccc gaagccggtc ggactcggcg tgcgccgtat cgatgaggcg cgtcgattcg
     1741 gcgttggcgg cctgcaccac ctcgttttgc gacaccagcc gctgttgttc cttgatgccc
     1801 tcttgcacgg ccttctcgta ggagatgttg ccgttttcca ttagtcggtc gcattcggcc
     1861 tgggcgcgtc cgacgctggc ctcgtactcg cgtttcgacg ctgtggcgat gcggatcgac
     1921 tcctcgcggg cctcgccgac catccgctcg ctgtgttggc gggcctcgct caccatccgg
     1981 tcggcctgcg ctttcgcgtc ggaaaggagc cggtcggctt ccgcgcgggc gtggttgagc
     2041 atcgactccg actccgtggt cgccgtggaa accatggatt cggcgtgtgt cttggcgtca
     2101 tgcagcatcg aatcacgcgc gtccagcaca tcctgggcgt cgtccagctc gccggggatc
     2161 gcatccttga tgtcgtcgat cagctccagc acgtcaccgc gcgggacgac acagccggac
     2221 gtcatcggca cgccacgggc ctcctcgaca atggcaccca attcgtccag cgcctcaaaa
     2281 actcggtaca cggccatcgc ctcctggcgt cttcgcaaaa atcctgttgt taccagtgtg
     2341 cctggtgtta cgtctgtgac agtggtggca cggccggtgt gtcgggcatt tgcccggcac
     2401 agctcacccc gagctcaccc ggtcacgcaa cccaggcacc cgcgcattcc cgaagcccat
     2461 cttccctacc cggcaatacg cagccgaagc ctacgccgct caggttcctc gagcgcaggt
     2521 cacggcaacg cccgcgacgg ggcgggcgac ccgtgacctc cgagataggc tggctggcgt
     2581 gagcgggccc acccaaccaa gcaaagacga cgcgagaccg gcggtgccga ccctctatat
     2641 tttcccgcac gccggtggct ccgcgacctt ttatgttccc ctcgccaagg cgttctccgg
     2701 cggcgtcaaa accattgccg tgcagtatcc gggacgacgc gatcggcaag ccatgactca
     2761 gctcaccagc atccccgccc tggccgaaga aatattcacc atgatggcgc cctccgccgg
     2821 gaccgacggt cacgtggcct tcttcggtca cagcatgggc ggactgctgg ctttcgaagt
     2881 ggccctgagg ttccaatcag ccgggcaccc gatctcggcg ctttttgtct cgtcgtgcgc
     2941 tgcccccggc catatccggt acaaggaatt acagggatct gatcgcgaca tcttgaatct
     3001 ggtcgccgcg gcgaccaata cgaatcccga gctgctggcc actgatgaat tcgccgccac
     3061 cgttttgccg acattgcaaa gcgtccgcgc cattgccgag tacacccgtc cggccgagtt
     3121 gaccgtatcg tgcccgatct acgggtttct cggggacaac gacatcatgg tcgcccgcga
     3181 gaatatggag ccctggcgcg atcgcacctc ggcggatttt tcggtgcgga tgttcgccgg
     3241 agaccatttc tacttcaatc gacatttgcc ggaattggtc aaagagatcg aggaaaacgc
     3301 gcttcgtccc tgcccgagaa gttagctcaa ccgacgctca cgaggtctcc tggccggact
     3361 cgcacggtga ctgccagaag tttgccggaa gccacgaccc acggcgggtg aacggggaac
     3421 ttcggcacga gcgtcccgca gccggcccag tatcggaaag tctcggaccc ggcgccaacc
     3481 gggcccgccg caggcaccgg cagcaccgcg gcccgacggg gaggccgcag ccgaaacggt
     3541 gcgccagtgc caaaacggac gatctgtgtc gcatctcaca tctgttccca aacgctgctg
     3601 cctcacccca cacatcagcc gcgacgacgc tgatttcagc ggaaaagtat ggaataagat
     3661 gcgatcacgc tccgcgaact tagggccaag ctgtggattt cttaaatctc gtcgcttaag
     3721 cggcaaaaca cgcttaccgt gcttccaagg ggctgaatgc ggggaaaaga aaaaatcgac
     3781 ctcccagtat cccggccctt ataggtacgg cgctgccacc acgaggcgcc gtgcgctacc
     3841 gcaccgtgcg aggacttcga cagctcacac aggcacaggc ccggggggcc tataggccgc
     3901 cgcaatgctg ctcgacaggt gcctcacacg gcgcgtgtcc ataatgaacg ccggggggcg
     3961 aaacgggaag gtattaagtg ccgttgttac cctcgtccat ccctgcgttg ctgcaggcac
     4021 gcgccgaaca gcaaccggac gccccggctt tcacctacgt cgactacgga caagaccccg
     4081 gtgggtttgc tgagagtctc acatggtcgc aggtttaccg ccgtgcccgc acgcttgctg
     4141 aggagcttgc cctgtggggc tcgcccggag accgagtggc gatcttggcg ccgcaggggc
     4201 tggaatacgt cgtcgccttc ctcggtgccc tgcaggcggg attcgtcgcg gtgccgctct
     4261 caacacccca atatggcatt cacgacgacc gggtttcctc ggtgttgcgt gattcccagc
     4321 cggtggccat tctcacgact tccgctgtgg tcaccgacgt ggccaaatac gccacgggac
     4381 aggacggacg acccgcgccg ctcgtcatcg aaatcgacct gctcgacctg gattcaccgc
     4441 acgaaatgtc gcgcacggcc gaatttttct ccggcgcagc ctatctccaa tacacgtcgg
     4501 ggtcgacgcg cgcaccggcc ggcgtcgtcg tgtcgcacaa gaacgtcatc gccaacgtca
     4561 cacagagctt gtacggctat ttcggcgatc ccgcaaaggt tccgaccggg tctgtggtgt
     4621 cttggctgcc cctgttccac gacatggggt tgattctcgg catctgcgcc ccgatggtgg
     4681 ccggccgcag cgccgtgttg ctgagcccga tggcgtttct gcgtcgcccg gcctgctgga
     4741 tgcagctgct tgcgaccagc ggcgagtgct tttccgcggc accgaatttc gccttcgaat
     4801 tgaccgtgcg caggacatcg gacgacgaca tggccgggct cgacctgggc aaggtggttg
     4861 ggatcgtcag cggcagtgag cgaatccacg tcgccacggt caagcgtttc accgagcgtt
     4921 tcgccccgta caacttgcgg cccgcggcgg tacgaccctc ttacgggctc gcggaagcga
     4981 ccctttacgt ggcggccccc gaagccggca ccgcccccaa gactgtccgt ttcgactacg
     5041 aagccttgac cgcgggcgag gccaggacct gcggagtcga ggggactatc gggaccgagc
     5101 tggtcagcta cggctcgcct gagccatcgt ccgtgcgaat cgtcaatccg gagaacatga
     5161 ttgagagtcc gccgggagtc gtcggcgaaa tctgggtgca tggcgatcac gtggccatgg
     5221 gatattggcg caagccggag cagaccgcgc gtacgttcaa cgccgcgttg gtcgaccccg
     5281 cgccgggaac cccgaagggg ccgtggctgc ggaccggaga cgtgggagtc atgtccgacg
     5341 gcgaactgtt catcatgggc cgcatcaagg accttgtcat cgtggacggg cgcaaccact
     5401 atcccgacga catcgaggcg acgatccggg aaatcaccgg aggccgggtc gcggcgatat
     5461 cggtgcctga cgacctcacc gagcaattgg tcgcgatcat cgaactgaag aggcgcggag
     5521 catccgccga agaggcaatg ctcaaacttc gctcggtgaa gcgtgaagtc acatcggcga
     5581 tctcgaaatc gcacagcctg cgcgtcgccg atcttgttct ggtgtcgccc ggttcgatac
     5641 cgatcaccac gagcggcaag atccggcgtt cggcctgcgt cgaacggtat cgaagcgacg
     5701 gcttcaagcg gctggacgtc tctgcatgac atcgggcatc aacaacgaag ccgacttgcg
     5761 tcactggctg gtggactacc tggtcacgaa tatcggctgc accccagacg aggtcgatcc
     5821 cgatctgtca ctggccgacc ttggtgtgtc atcccgcgac gcggtggtgc tgtccggcga
     5881 attgaccgag gtgttgggca ggaccgtctc tcccattgac ttttgggagc acccgacgat
     5941 caacgcgctg gccgcctacc tcaccgcgcc acagcccggg cccgaatggc aagccgcact
     6001 cgagcgcccg gctcgaagcc tcctcgagga gccgatcgcc gtcatcggaa tgggctgccg
     6061 cttccccggc ggcataagag ggccagaggc gttgtggcag ttcctcattg accgccgttc
     6121 ttcgatcggg cgggtccccg acgaacggtg ggagctgttc gacgacggct cgccggaagt
     6181 gaaggcactg ctggcccgca ccacgcgttg gggctcattc ctgcccgaca tcgacgcctt
     6241 tgacgcggaa ttcttcgaga tctccccgag tgaagccgac aagatggatc cgcagcagcg
     6301 cctgctgctg gaagtggcct gggaagcgtt ggagcacgcg ggaattccgc ccaactcgct
     6361 gcgccgttcg cagacggggg tgttcgccgg ctcatgcctg agcgaatacg gcgccatcgc
     6421 gtccaccgac ctgccgcagg tggacggctg gagcaacacc ggtggtgcga tgagcattat
     6481 cgccaaccgc ctctcgtatt tcctcgacct gcgcggcccc tccgtagcga tcgacaccgc
     6541 atgctcatcg tcgttggtgg cgatccacct ggcctgccag agcctccgaa cccaggactc
     6601 gcacctagcg atcgccgccg gcgtcaattt gttgttgtct ccggccgtat ttcgcggttt
     6661 cgaccaagtc ggtgcgttgt cgccgaccgg tcgttgccgc gcgttcgatg cggccgccga
     6721 cgggtttgtc cggggcgaag gcgccggggt tgtggtgctc aagcggttga ccgacgcgca
     6781 acgcgacggt gatcgggtgc ttgcggtcat ctgcggttcg gcggtcaacc aggacggccg
     6841 ttccaacggg ctgatggccc ccaacccggc ggcccaaatg gcggtgctgc gcgccgccta
     6901 cgccaactcc gggatgcagc cgcatgaggt cgactatgtc gaagctcacg gaaccggaac
     6961 cctgttgggc gatccgattg aggcacgcgc cctgggcacg gtgctgggtc gcgggcgtgc
     7021 cgaggatgct cccctgctca tcggcgccgt caaaaccaac ctcggtcaca ccgaggcagc
     7081 ggccgggatt gcgggcttca tcaagacggt aatggcggtg cagcgcggtc agattccgcc
     7141 gaatcagcgc ttcgagagcc cgaacccgca cattcccttt gccgacttgc ggatgaaagt
     7201 cgttgatacc caaactgaat ggccggacac ggggcatccg cggcgggcgg gtgtgtcgtc
     7261 attcggattc ggcgggacga acgcacacgt ggtgatcgag cagggccagg aggtggccac
     7321 gcaacctcgg ccgggttcgg atcccggagt ctcgacgctg gtggtctcgg gtaagacgcc
     7381 gcgacgggtg gcggcgacgg cggccgtgct ggccgattgg atggaggggc ctggagccca
     7441 ggtgccgctg gccgatgtgg cccacaccgt caaccatcac cgcgcccggc aagccaagtt
     7501 cggcaccgtg gtcgcgcgcg agcgcgtcca cgcagtggcc gggttgcggg ctctggccgc
     7561 cggccggcgc gcccccggtg tggtctgccc ccaagaaagc actccggggc cgggcatggt
     7621 gttcgtctac tccggccgcg gttcccagtg ggccggcatg ggccgccaat tgctggccga
     7681 cgagccggct ttcgccgccg cgctcggcga actcgaaccg gtgttcgtcg aacaggccgg
     7741 cttctcactg cgcgacgtgg tcgccaacgg cgaggaactg gtcggtatcg agcagatcca
     7801 gctgggcctg atcggcatgc agctggcgct gaccggcttg tggcgttcct acggggtgca
     7861 ccccgacctg gtgatcggcc actcgatggg tgaggtggcc gccgccgtcg tcgcgggtgc
     7921 gctcacgccc gccgaggggc tgcgggtgac cgcgacccgg tctcggctga tggcgccgtt
     7981 gtccggacac ggtggcatgg cgctgctcga gctcgacgcg gccgcgaccg agacgttgat
     8041 cgccgggtac ccccaggtga cgttgggcat ctacaactcg ccgcgccaaa ccgtgatcgc
     8101 cgggcccac
//
LOCUS       DASSGQ010000131         5875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1225168,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000131 DASSGQ010000000
VERSION     DASSGQ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225168"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="VFI55_06155"
     CDS             <1..157
                     /locus_tag="VFI55_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal ligand-binding domain-containing protein"
                     /protein_id="HET7073651.1"
                     /translation="RGEAHPDVDPDRLIELIGGATMLRMLLRPQEELDDTWVEQTTAI
                     VVHGVTG"
     gene            complement(213..818)
                     /locus_tag="VFI55_06160"
     CDS             complement(213..818)
                     /locus_tag="VFI55_06160"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7073652.1"
                     /translation="MPPCRDLAAFNDRAPEYDRGWRGRLHHEIAARTACLAVATVPSP
                     GRVLDVGCGTGYLLRSLAHRYPHADQLCGIDAAPQMISTASASAGDERLTFTVGVAEG
                     LGFLDGTFDLIVSTTSFDHWSDQQAGLVECVRVLRPGGHLVLVDQFSRWLLPTVATTR
                     RGKARTRTRATALLLRAGLRSLQWHRVYAVIINGVKAAKPA"
     gene            complement(845..2216)
                     /locus_tag="VFI55_06165"
                     /pseudo
     CDS             complement(845..2216)
                     /locus_tag="VFI55_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772906.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="13E12 repeat family protein"
     gene            2440..3384
                     /locus_tag="VFI55_06170"
     CDS             2440..3384
                     /locus_tag="VFI55_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HET7073653.1"
                     /translation="MPGIDKPTGRVVALIVLMIFVAAALRGYLPAEDRAVRGEPGSSP
                     AAMIFVVAVLCAALTLLTIAVIARLRDPRAMAPSAQLPSEMAGGGKGRPNWRVLLIAL
                     VVIATWLLIMLLLVRWFVPHTSTPDVSSSAPIAPPSGHAAGASSRRHPQNNTGDMFAI
                     LVASAIPMLLVIVVGSMIKWLRRWRAATPGAVVGDHFESPAPVPSSESLVRAAEVGLA
                     EIGDRDREPREAIIACYAAMERELANVPEAAPQDFDTPTEVLVRAVERHALHADNAAQ
                     LVNLFEEARFSPHVMNEGHREVAVHVLRLVLAELRSPV"
     gene            3381..3863
                     /locus_tag="VFI55_06175"
     CDS             3381..3863
                     /locus_tag="VFI55_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073654.1"
                     /translation="MKRLLVLGSCLIVGTELLALMLHDRALVLAASGVALALVLLNVR
                     RFVGLGTQPAAEPNPDDLGDSLRRWLSTTETTIRWSDSTRADWDRHLRPMLARRYELT
                     TGQKQSKDPVAYHASGRMLFGAELWEWVNPNNVTRTGDRQPGPGRGALEEILRRLEQV
                     "
     gene            3894..4874
                     /locus_tag="VFI55_06180"
     CDS             3894..4874
                     /locus_tag="VFI55_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET7073655.1"
                     /translation="MTLPAAMPAGKTTAYCEAVLDEIERVVVGKRSALTLILTAVLAR
                     GHVLIEDLPGLGKTLIARSFAAALGLRFTRVQFTPDLLPADLLGSTIYDMQSGHFAFR
                     AGPIFTNLMLADEINRTPPKTQAALLEAMAERQVSIDGETHKLPTPFIVLATDNPIEY
                     EGTYPLPEAQLDRFAMRLELRYLSEQDETSMLRRRLERGSAEPTVNQVVDAHDLLAMR
                     ESVEQVTVHEDVLHYVVSLATATRHHPQVAVGASPRAELDLVQLARARALLLGRDYVI
                     PEDVKALAVAAVAHRITLRPEMWVRKIQSADVVGELLRRLPVPRTRGGTG"
     gene            4882..>5875
                     /locus_tag="VFI55_06185"
     CDS             4882..>5875
                     /locus_tag="VFI55_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET7073656.1"
                     /translation="MIASQEVELRWRASELTLAIATCAGVALAAAVLGARWQLIAFAA
                     PLLGVLCSISWQRPAPKIRVHGEPDAQRCFENEQARLTLWATVESGVSTPRGGAASSV
                     IDLTVSTVAGMELTVVDADSGHAKTVAAKAQRWGRYPIRAGVEVVARGGLLTGTASVD
                     AVEVIVFPLTPPQSTPIPQTELLDRLGAHLTRNIGPGVEYADIRPYVPGDQLRAVNWP
                     VSARRGRLHVTERLTDRAADVVVLIDTYRQPAGPATEATERAVRGAAQVVQTALRHGD
                     RAGIVALGGNRPRWLGADIGQRQFYRVLDTVLAAGDRFENTTGTLAPRAAVPAGA"
BASE COUNT          910 a         1916 c         2084 g          965 t
ORIGIN      
        1 ccggggcgag gcgcatcccg atgtggaccc ggaccgcctg atcgaattga tcgggggcgc
       61 gacgatgctg cggatgctgc tgcgcccgca ggaagaactc gatgacacat gggtggagca
      121 gaccaccgcc atcgtggtgc acggtgtgac tggatgacgg gcgcgcggtg gcgatccgtg
      181 ccgatcccac cgccgcggcc gtgcacgctg cgttaagccg gcttggccgc cttcacgccg
      241 ttgataatca cggcatacac cctgtgccat tgcagcgaac gtaatcccgc gcgcagcagc
      301 agggccgtgg cgcgagtcct ggtgcgggcc ttgccccgtc gagtggtggc cacggtcggc
      361 aacagccacc gcgagaactg gtcgacgagc accaagtgac cgccgggacg caagactcgg
      421 acgcattcga cgaggcccgc ttgctggtcg ctccagtgat cgaaagacgt cgtgctgacg
      481 atgaggtcga aagtgccgtc gaggaagccg agtccttcgg cgacgccgac agtgaaggtc
      541 aatcgctcat caccggcgga agcgcttgcc gtcgagatca tttggggtgc ggcgtcgatg
      601 ccgcaaagct gatcggcgtg cgggtaacga tgggcgaggc tgcgcagcag gtaacccgtc
      661 ccgcaaccca cgtcgagcac gcggccggga gaggggacgg tcgccaccgc gagacaggcg
      721 gtgcgagcgg caatctcatg gtgcagccgg ccacgccagc cgcggtcata ttcgggcgct
      781 cggtcgttga atgcggccag gtcacggcac ggcggcaccg gtccagtgtt tcaccgtcgg
      841 cgtgttaccg cccagggtcg gggtcgccac gcagcagttc ttcggggtgg tgcgcgtgat
      901 tgatttccgg cggaccgttg gcggcggtcc agccaagccg accgcccccg gtgatgacgg
      961 tgcgcaattc gccccggctc accgccgcgt ggtcgcgacc gcaagcgaaa aatagcttgt
     1021 cggcatcggt ccggccacct tccgcccaat ccggcgaatg gtgcgcctcg ctgcgatacc
     1081 cggattcgag gcacttcggg cgggtgcagc cgtggtcgcg ggcgtaacag atgagccgtt
     1141 ggtccgcggt ggcgatgcgt ttaacacccc agatacaagg gccgttcggt gtgctcgtcg
     1201 aacacggcca agtagtggat tgctttggcg gccatgcgaa tcagatcccg catcggcagc
     1261 ctcgagccac ctcccgtgtg ggccggcgcg ggcagcggga cggttgggtc ggcgacggcg
     1321 tgtgcggccc ggttgaggtc ggccagcgtg gtggtggcga tgacggtgac tgaatgaccg
     1381 cggtgcaccc cgagcttgcc ggacgcaatg gcggcttcca ggccgagttt gagcgcgtcg
     1441 tggcaccgct gcgcgggagt gcgctggtct cgctcttggg tctgggcgct atcggcttgg
     1501 tgacggccgg gccgcacggc cgcgtcaatg gcttcgaagt acgcgcgggc ctcggggtcc
     1561 agcaggcccg tcaaccggga catgccgtcg ggcccctgcg ggctgagacg cagtccgcgg
     1621 cgacgcgccc ggtcgtcctc gttgaacagg ccgtcgggat tgaggtagtc ggcgatccgc
     1681 tggccgagcc tgatgacgat gccggcgtcg agtttggttg cgtggcccac cagcgtgcgc
     1741 tcggcttcgg cgacatcggt gggcgagacg caggcgggca gcacatcgac cgcttcgcat
     1801 atcgcgcgga cgtggtcctc gccgacggtg cccgcttcca ccgcggccgc cagctccggc
     1861 agttccggct gcagcggggg accggtcagc gtgcgccgcg gccgaatccg ggccgcgagt
     1921 ttgaagcggc gaacgatctc cctgggctgc acgcgcagcc gcgcccgcaa cgcctctcgg
     1981 accgccggta tgcactcgct gcagtcgggt ggctcggcga tctcggcgaa gaaccggtac
     2041 atcagtccgc ggttggtgcg gtcctgcgtc tccagccgct cggcgacgtc gacgcggaaa
     2101 ttgttgccga ccagatcgga gccggtttcg cacagcatgg cgtgcgcggc atcgatcgcg
     2161 tcgagagcgg cgctgatccg ctcccgcgct tcctcggcgc tcgcgcagaa atccatgccc
     2221 cgaacgctac gaaccgccac cgtcgaattc cgtgggccgc gacggcgtcg ttgccggcct
     2281 gtggatgaat cggcgactgt caataaagtc ttcgagcgtc ttctggcgtg gcccggccca
     2341 ggtagcggcc gtgttcctgg cggaatggtc gcctgacgtg ccagtatgaa gaaagccagt
     2401 gccgcaacgg cggcaggcaa ccggtcgagt ggtgtgccaa tgcccggtat tgacaagccc
     2461 acggggcgcg ttgtggcgct gatcgtgctg atgatcttcg ttgctgccgc gctgcgcgga
     2521 tacctgccgg ccgaagaccg cgcggtgcgc ggggagcccg gcagcagccc ggcggcgatg
     2581 attttcgtcg tcgccgtcct ttgcgcggcg ctcacgctgc tgacgatcgc ggtcatcgcg
     2641 cggttgcggg atccacgtgc gatggcgcca agtgcgcagc tcccgtccga aatggccggc
     2701 ggcggtaagg gccgaccgaa ctggcgggtg ttgctcatcg cgctcgtggt gatcgcgacc
     2761 tggctgctga tcatgctgct gctcgtcaga tggttcgtgc cgcacacgtc cacgcccgac
     2821 gtgtccagct cggccccgat cgcgccgccg tcggggcatg ccgcgggtgc gtcgtcgcgg
     2881 cgacacccgc aaaacaacac cggggacatg ttcgcaatcc tggttgccag cgcgatcccg
     2941 atgctgctgg taatcgtcgt cggatcgatg atcaagtggc tgcgacggtg gcgcgcggcc
     3001 acaccgggag ccgtcgtcgg cgatcacttc gaatcccccg caccggtgcc gagttcggaa
     3061 tcgctggttc gcgcggccga agtgggattg gcggagatcg gcgaccgcga ccgcgaaccg
     3121 cgcgaggcga tcatcgcgtg ctacgccgcg atggagcgtg agctcgccaa tgtgccggaa
     3181 gccgcgccgc aggacttcga cacgccgact gaggtgctgg tccgcgccgt cgagcggcac
     3241 gcgctgcacg ccgacaacgc ggcccagctg gtgaacctct tcgaggaggc gcggttcagt
     3301 ccccatgtga tgaatgaggg gcaccgtgag gtggcggtgc acgtgttgcg gctcgtcctc
     3361 gccgaattgc ggagcccggt atgaagcggc tactggtgtt gggcagttgt ctcatcgtcg
     3421 ggaccgagct gttggcgctg atgctgcacg atcgcgcatt ggtgttggcc gcctccggtg
     3481 ttgccctggc tctggtgctg ctcaacgtcc gtcgtttcgt gggactcgga acccagcccg
     3541 ccgccgagcc caaccccgac gatctggggg attcgttgcg ccgctggctt tccaccaccg
     3601 agacgacgat ccgctggtcg gactccaccc gcgcggactg ggaccggcac ctgcgtccca
     3661 tgctcgcgcg gcggtacgag ctgacgacgg gccaaaagca gtccaaagac ccggtggcat
     3721 atcacgcaag cggccggatg cttttcggcg ccgaattgtg ggaatgggtt aatccgaaca
     3781 acgtcacgcg caccggcgat cgtcagcccg gccccggccg cggagcgctg gaagagattt
     3841 tgcgaaggtt ggagcaggta tgagcggagg cgcgacacgg atatgacact gacatgacac
     3901 tgccggccgc catgcctgct ggtaagacca ccgcttactg tgaggcggtg ctcgacgaga
     3961 tcgagcgtgt ggtggtgggt aagcgctccg cactcacgct gatcctcacc gccgtcctcg
     4021 cgcgcggcca cgtgctcatc gaagacctgc cgggcctcgg caagacgctg atcgcgagat
     4081 cgtttgccgc cgcattgggg cttcggttca cccgggtgca gttcaccccc gatctgctgc
     4141 ccgcggacct gctgggttcg acgatctacg acatgcagtc gggccacttc gcgttccggg
     4201 cgggccccat cttcaccaac ctgatgcttg ccgacgagat caaccgcacc ccgccgaaga
     4261 cgcaagcggc cctgctggag gcgatggccg agcgtcaggt gagcatagat ggcgaaaccc
     4321 ataagctgcc aacgccattc atcgtcttgg cgaccgacaa tccgatcgag tacgagggca
     4381 cctatccgtt acccgaggcg cagctggacc ggttcgccat gcggctggaa ctgcgctatc
     4441 tctcggagca ggacgagact tcgatgctgc gccgccgcct cgagcgtggt tcggccgagc
     4501 cgacggtcaa tcaagtggtc gacgctcacg acctgctggc gatgcgcgaa tccgtcgagc
     4561 aggtgacagt gcacgaagac gtgttgcact atgtggtgtc gctggccacc gccaccaggc
     4621 accatccgca ggtcgccgtg ggcgccagcc cacgcgccga actcgacctg gttcagctcg
     4681 cccgcgcccg tgcgctgttg ctcggtcgtg actatgtgat ccccgaggat gtgaaggcgc
     4741 tcgcggttgc ggcggtcgcg caccgcatca cgctgcgacc ggagatgtgg gtgcgaaaga
     4801 ttcagagcgc ggatgtcgtc ggagaattgt tgcggcggtt gccggtgcct cgcacccgcg
     4861 gggggaccgg atgagcgcgg cgtgatcgcc agccaagaag tcgagctccg ctggcgcgca
     4921 tcggaattga cgctggcgat cgcgacgtgc gccggggtcg cgctggcggc cgccgtgcta
     4981 ggcgctcgct ggcagctgat cgccttcgcg gcaccactgc tcggtgtgct ctgctcgatc
     5041 agctggcagc gcccggcgcc gaagatccgg gtgcacgggg aaccagacgc ccagcgctgc
     5101 tttgaaaacg agcaggcgcg gctgaccctg tgggcgacgg tggagtccgg ggtgtccaca
     5161 ccgcggggcg gcgccgcgtc ctcggtgatc gacctcacgg tgtcgaccgt cgcgggcatg
     5221 gaactcacag tggtcgacgc ggattccggc cacgcgaaaa ccgttgcggc aaaagcacaa
     5281 cgctggggcc gctatccgat ccgggccggg gtcgaggtgg tcgcgcgcgg tggattgctg
     5341 acgggaacgg ccagcgtcga cgccgttgag gtcatcgtgt ttccgctgac gccgccgcaa
     5401 tcgacgccca tcccgcagac cgagttgctc gaccgtctgg gggcccacct cacccggaac
     5461 atcgggccgg gtgtggagta cgccgacatt cgcccctacg tgcccggcga ccagctgcgg
     5521 gcggtgaact ggccggtgag cgcacgccgc ggccggttgc acgtgacgga gcgattgacc
     5581 gaccgcgccg ccgacgtcgt ggtgctgatc gacacgtatc ggcagccggc gggcccggcg
     5641 accgaagcca ccgaacgcgc ggtgcgcggc gcggcgcagg tggtgcagac cgcgttgcga
     5701 cacggcgatc gcgccggaat cgtcgccctc ggtggcaacc gcccacggtg gctcggcgcc
     5761 gacatcggcc agcgtcagtt ctaccgggtg ctggataccg tgttggccgc cggcgaccga
     5821 ttcgaaaaca caacgggcac actggcgccg cgtgccgccg tccccgccgg ggcga
//
LOCUS       DASSGQ010000132        12236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1072844,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000132 DASSGQ010000000
VERSION     DASSGQ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12236
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072844"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VFI55_06190"
     CDS             complement(<1..1080)
                     /locus_tag="VFI55_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7073657.1"
                     /translation="MIWGSRRRTRSRWGRSGPMTRGMGAVSRAVGIAWRRSLQLRVVA
                     LTLGLSLAVILALGFVLTSQVTNRVLDVKVKAAIEQIDRARTTVGGIVNGEETRSLDS
                     SLQLARNTLTSKTDLASGAGLAGAFDAVLMVPGDGPRAATAAGPVDQVPNALRGFVKA
                     GQAAYQYATVHTEGFSGPALIIGTPASSQVANLELYLIFPLTNEQATIQLVRGTMITG
                     GALLLVLLTGIALLVSRQVVVPVRSASRIAERFAEGHLSERMPVRGEDDMARLAMSFN
                     DMAESLSRQITQLEEFGNLQRRFTSDVSHELRTPLTTVRMAADLIYDHSADLDPTLAR
                     STELMVNELDRFETLLNDLLEISRHD"
     gene            complement(1181..1867)
                     /gene="mtrA"
                     /locus_tag="VFI55_06195"
     CDS             complement(1181..1867)
                     /gene="mtrA"
                     /locus_tag="VFI55_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator MtrA"
                     /protein_id="HET7073658.1"
                     /translation="MDLMRQRILVVDDDASLAEMLTIVLRGEGFDTAVIGDGTQALTA
                     VRELRPDLVLLDLMLPGMNGIDVCRVLRSDSGVPIVMLTAKTDTVDVVLGLESGADDY
                     IMKPFKPKELVARVRARLRRNDDEPAEMLSIADVEIDVPAHKVTRNGEQISLTPLEFD
                     LLVALARKPRQVFTRDVLLEQVWGYRHPADTRLVNVHVQRLRAKVEKDPENPTVVLTV
                     RGVGYKAGPP"
     gene            complement(1960..2592)
                     /locus_tag="VFI55_06200"
     CDS             complement(1960..2592)
                     /locus_tag="VFI55_06200"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET7073659.1"
                     /translation="MLIAIEGVDGAGKRTLTDALSGEFRAAGMSVATLAFPRYGRSVT
                     ADVAAEALHGEHGDLASSAYAMAMLFALDRAAAVEEIDRLGRDHDVVILDRYVASNAA
                     YSAARLHEDAAGAAVAWVHRLEYERLRLPPPDWQVLLAVPAELAGQRARSRAESEPGR
                     QRDSYERDDGLQQRTGAVYAGLAAAGWGGRWLVVDANVDPGWLTGLLRAP"
     gene            complement(2685..4163)
                     /gene="ahcY"
                     /locus_tag="VFI55_06205"
     CDS             complement(2685..4163)
                     /gene="ahcY"
                     /locus_tag="VFI55_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET7073660.1"
                     /translation="MTTTESSLTADVRNGIDFKIADLSLAEFGRKELDLAEYEMPGLM
                     SLRREYAEVQPLKGARISGSLHMTVQTAVLIETLVELGAEVRWASCNIFSTQDHAAAA
                     VVVGKHGTQEEPKGVPVFAWKGETLEEYWWAAEQMLTWPDPDKPANMILDDGGDATMM
                     VLRGAQYEQAGVVPPAEEDDSAEWKVFLSVLRNRFETDKEKWTKISESVKGVTEETTT
                     GVLRLYQFAAAGDLAFPAINVNDSVTKSKFDNKYGTRHSLIDGINRGTDALIGGKKVL
                     ICGYGDVGKGCAEAVKGQGARVVVTEIDPINALQALMEGFDVVTVEQAIGDADIVVTS
                     TGNKDIISLEHMKAMKDHAILGNIGHFDNEIDMAALERSGATKLNIKPQVDLWTFGDS
                     GKSIIVLSEGRLLNLGNATGHPSFVMSNSFANQTIAQIELWTKNDEYDNEVYRLPKHL
                     DEKVARIHVEALGGTLTKLTKEQAEYLGIDVEGPYKPDHYRY"
     gene            complement(4274..4879)
                     /locus_tag="VFI55_06210"
     CDS             complement(4274..4879)
                     /locus_tag="VFI55_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET7073661.1"
                     /translation="MKRIPYAEASRALLRDSVLDAMRDLLLIRDWSAITLSDVARAAG
                     ISRQTIYNEFGSRQGLAQGYALRLADRLVDAVHAALDANVGNIYEALLQGFRSFFAES
                     AADPLVISLLTGVAKPDLLQLITTDSAPIITRASARLTLALTETWVSTSEEDAGVLAR
                     AIVRLALSYVPMPPEADHDVAADLARLMTPFAERHGVVNIP"
     gene            complement(4957..5139)
                     /locus_tag="VFI55_06215"
     CDS             complement(4957..5139)
                     /locus_tag="VFI55_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HET7073662.1"
                     /translation="MSDHKLFRCIQCGFEYDEALGWPEDGIAPGTRWDDIADDWSCPD
                     CGAAKSDFEMVEVARP"
     gene            complement(5136..5312)
                     /locus_tag="VFI55_06220"
     CDS             complement(5136..5312)
                     /locus_tag="VFI55_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HET7073663.1"
                     /translation="MTAAYQCPVCDYVYDEAKGDVREGFPAGTRWAQIPDGWCCPDCS
                     VREKLDFQELGVTR"
     gene            complement(5309..6580)
                     /locus_tag="VFI55_06225"
     CDS             complement(5309..6580)
                     /locus_tag="VFI55_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HET7073664.1"
                     /translation="MTTLGPPVMREAQGAQVPVWRDKKRHLWLMGLIAPTALFVMLPL
                     IWGLNQLGWHVAAQVPLWIGPILLYVLLPILDLGFGPDGQNPPDEVMEQLENDKYYRY
                     CTYVYIPFQYLSVALGAYLFTATNLSWLGYVGPLSWVGKLGVALSVGVLGGVGINTAH
                     EMGHKKDSLERWLSKITLAQSCYGHFYIEHNRGHHVRVATPEDPASSRFGETFWEFLP
                     RSVFGSLRSAVRLEAQRIRRQGASPWNPKTYASNDVLNAWAMSVVLWGVLTAVFGPAL
                     IPFVIIQAVFGFSLLEAVNYLEHYGLLRQRHDTASGKSRYERCTPVHSWNSDHVVTNL
                     FLYHLQRHSDHHANPTRRYQTLRSVAGAPNLPSGYASMISLTYFPPLWRKVMDHRVLA
                     HYDGDITRVNLQPRLRDKLLARYGASHEAQR"
     gene            complement(6692..8200)
                     /locus_tag="VFI55_06230"
     CDS             complement(6692..8200)
                     /locus_tag="VFI55_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET7073665.1"
                     /translation="MANRWRTKSVEQSIADTDEPDTRLRKELTWRDLAVFGVAVVIGA
                     GIFTVTASTAGDITGPAIWVSFVIAAITCALAALCYAEFASMLPVAGSAYTFSYAAFG
                     EFLAWIIGWNLVLELAIGAAVVSKGWSSYLGTVFGFAGGTVTFGSVQLDWGALLIVAV
                     VATLVALGTKLSSRFSAVITAIKVSVVVLVVVVGLFYIKGSNYSPFIPKPESGGDASG
                     INQSVLSLLTGAHSSHYGWYGVLAGASIVFFAFIGFDIVATMAEETKRPQRDVPRGIL
                     ESLAIVTVLYVAVSVVLSGMVSYTQLKTVAGHQPANLATAFTANGVQWASKIIAIGAL
                     AGLTTVVMVLVLGQCRVLFAMARDGLLPRALAKTSSRGTPVRITVLVAIVIAATASVF
                     PIDKLEEMVNVGTLFAFVLVSAGVMVLRRSRPDLERGFRAPWVPWLPIASICACVWLM
                     LNLTALTWVRFAVWLVVGTAIYGGYGYRNSVQGRRQAGQLPETARMGAATER"
     gene            8302..9681
                     /locus_tag="VFI55_06235"
                     /pseudo
     CDS             8302..9681
                     /locus_tag="VFI55_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217771.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            complement(9659..10885)
                     /gene="manA"
                     /locus_tag="VFI55_06240"
     CDS             complement(9659..10885)
                     /gene="manA"
                     /locus_tag="VFI55_06240"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HET7073666.1"
                     /translation="MELLRGALRTYAWGSRTAIAEFTGRPVPAAHPEAELWFGANPGD
                     PAWLETDKGEISLLDAVVADPEGQLGAGSRARFGDVLPYLVKVLAADEPLSLQAHPSA
                     AQAVEGYRREERMGIPVSSPVRNYRDTSHKPELLVALLSFEALAGFRPVSRTIELLRA
                     LAVSDLDPFIELLNDRSDAGGLRALFTTWITAPQPDIDVLVSAVLDGAIAYVSSGARE
                     FAPEAKTVLELGERYPGDAGVLAALLLNRISLAPGEAIFLAAGNLHTYLRGFALEVMA
                     NSDNVLRGGLTPKHVDVPELLRVLDFAPTTEAQLRAPVRREGLQRLYETPTDEFAVAL
                     LALDGEYVGHEVDAPSHHEGPQILLCTEGCTTVHGKSGSLTLEHGMAAWVAADDGPIR
                     LVAREPAKLFRATVGL"
     gene            complement(10894..12027)
                     /locus_tag="VFI55_06245"
     CDS             complement(10894..12027)
                     /locus_tag="VFI55_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TobH protein"
                     /protein_id="HET7073667.1"
                     /translation="MNATRAIDLEDTGGLLAADREGLLRAASSAGAQVRAIAAAADEG
                     ALDALRTQDRPRSLIWVAGRGTAETAGAMLAATLGGTASEPIAVVAEAPPWVGPLDVL
                     IVAGDDPGDPALVAAAATGVRRGARVVVVAPYEGPLRDATAGRAAVLAPRMWIPNEFG
                     LCRYLAAGLAVLKIVDPRPSIDLALLADELDAEALRNSTGRELFTNPAKTIAARMAGR
                     RVALAGGNAATLALARHGSSVLLRLAHEVVAATGLADALVALRGGLADEFSDTASSVD
                     ALFHDEEIDGPLPRRLRVLALALAAEQTVVAAGIAGFDGVDLVTAGDVPDVPDAAAGP
                     VGPDGVAASGVRAPTGAGRVEQQLAVLAVRLEMAAVYMRLVRG"
     gene            complement(12024..>12236)
                     /gene="manB"
                     /locus_tag="VFI55_06250"
     CDS             complement(12024..>12236)
                     /gene="manB"
                     /locus_tag="VFI55_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217774.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HET7073668.1"
                     /translation="GRIQSIDHLDGVTVDLGDGSWLNLRSSNTEPLLRLNVEARSTDD
                     VDAVVAQVRAEIAATVTQAARDEAGS"
BASE COUNT         2040 a         4234 c         3963 g         1999 t
ORIGIN      
        1 gtcgtgccgg gagatctcca gcaggtcgtt gagcagcgtc tcgaagcggt ccagctcgtt
       61 gaccatcagc tcggtcgagc gggccagtgt ggggtcgagg tcggcgctgt ggtcgtatat
      121 caggtcggcg gccatccgca cggtggtcag cggggtgcgc aattcgtggc tgacgtcgga
      181 cgtgaagcgg cgttgcaggt tgccgaactc ctcgagctgg gtgatctgcc gggacagact
      241 ctcggccatg tcgttgaacg acatggccag ccgtgccatg tcgtcttcgc cgcgcaccgg
      301 catccgttcg gagagatggc cttcggcgaa ccgctcggcg atccgcgagg ctgaccgcac
      361 cggcaccaca acctggcggg aaaccagcag cgcgatgccg gtgagcagca ccaacagcag
      421 ggcgccgccg gtgatcatcg tgccgcgcac cagctggatc gtggcctgct cgttcgtcag
      481 cggaaagatc aggtacagct ccaggttggc cacctgtgac gacgccgggg taccgatgat
      541 cagcgccggg ccggagaagc cttcggtgtg cacggtggcg tactggtagg ccgcctgccc
      601 ggccttgacg aaaccacgca gggcgttggg cacctgatcg acgggtccgg ccgccgtcgc
      661 ggcgcgcggg ccgtcacccg gaaccatcag caccgcatcg aacgcgcccg ccagcccggc
      721 gcccgaggcg agatcggtct tcgacgtcag ggtgttgcgg gccagctgga gactgctgtc
      781 gagcgaccgt gtctcctcgc cgttgacgat cccgccgacg gtggtgcgcg cccgatcgat
      841 ctgctcgatg gcggccttga ccttgacgtc cagcacacgg ttggtgacct ggctggtcag
      901 cacgaaaccg agcgcaagga tcaccgccaa tgagagcccc agggtcagcg ccacgacccg
      961 cagctgcagc gatcggcgcc aagcaatgcc gacggcgcga ctcaccgcac ccatgccacg
     1021 agtcatgggg ccggagcgtc cccagcgact ccgagtgcgt cgccgcgagc cccagatcac
     1081 ctgcgcggct cctcaatact gccgtgcgct gcgtcgtcgc cggcggtccc tatgcgcggc
     1141 tcttcggtat cgctccgttc ggcgtcgtcg ccgacgcgga tcacggaggt ccggccttgt
     1201 aacccactcc tcgaacggtc aacaccacag tcgggttctc aggatctttc tcgaccttgg
     1261 cccgcaggcg ctggacgtgc acgttcacca agcgggtatc cgcagggtga cgatatcccc
     1321 acacctgttc gagcagcaca tctcgagtaa acacctgacg cggtttgcgt gccagcgcga
     1381 ccaacaggtc gaactccagc ggtgtcaggg agatctgctc gccgttgcgg gtgaccttgt
     1441 gtgcgggcac gtcgatctcg acgtcggcga tggacagcat ttcggcgggt tcgtcgtcgt
     1501 tgcgccgcag ccgtgcccgt acccgggcga ccagctcctt gggcttgaac ggcttcatga
     1561 tgtagtcgtc tgcgcccgac tccagaccca gcaccacgtc cacggtgtcg gtcttggcgg
     1621 tcagcatcac gatcggaacg cccgagtcag agcgcaacac ccggcacacg tcgatgccgt
     1681 tcatgccggg caacatcagg tccagcagca ccaggtcggg gcgcaactcg cgcaccgcgg
     1741 tcagggcctg agtgccgtct ccgatgaccg cggtgtcaaa gccctcccca cgcagcacga
     1801 tggtgagcat ctcggccaac gaagcgtcgt catcaacgac caaaatcctt tgcctcatga
     1861 ggtccatggt gtcaccagat cgggataaaa ctcgggcacc acacgggcgt ttcttggttg
     1921 acagggccaa atactgtcaa atttcgccct taaaccagtc tacggggctc tcaacaggcc
     1981 agtcagccag cccggatcga cgttcgcgtc gaccaccagc catcgtccac cccagcctgc
     2041 ggcggccagc ccggcataga cggcgcccgt gcgctgctgg agtccgtcgt cgcgttcgta
     2101 actgtcgcgc tgccggcccg gctcggattc ggcgcggctt cgggcccgct gtccggcgag
     2161 ctcggccggc accgcgagca gcacctgcca gtcgggcgga ggcaggcgca gccgctcgta
     2221 ttcgagccga tgcacccacg cgacggccgc ccccgccgcg tcttcgtgca ggcgcgcggc
     2281 gctgtaggcc gcgttggagg cgacgtaccg gtccagaatc accacgtcgt ggtcgcggcc
     2341 caaccggtcg atctcttcga ccgccgcggc gcggtcgagc gcgaagagca tcgccatcgc
     2401 gtacgccgac gacgcgaggt ctccgtgttc gccgtgcagc gcctcggccg cgacgtcggc
     2461 ggtcaccgaa cgcccgtaac gcgggaaggc cagcgtggcc accgacatcc ccgcggcccg
     2521 gaactccccg ctcagagcat cggtgagggt ccgcttgccg gcgccgtcaa cgccttcgat
     2581 cgcgatcagc acggcgcgag cctatctgtt gccttgagcg tgacgttgca gtcacactca
     2641 gcgccgaacg tgactgcaac gtcacgttcg gcgaccaaaa gggctcagta gcggtagtgg
     2701 tccggcttgt aggggccctc gacgtcgatg cccaggtatt cggcctgctc cttggtcagc
     2761 ttggtgagcg tgccacccag cgcctcgacg tggatgcggg ccaccttctc gtccaggtgc
     2821 ttggggagcc ggtacacctc gttgtcgtac tcgtcgttct tggtccacag ctcgatctgg
     2881 gcgatcgtct ggttggcgaa gctgttgctc atcacgaacg acgggtgccc ggtggcattg
     2941 cccaggttca gcagccggcc ctcggacagc acgatgatcg acttgccgga atcgccgaag
     3001 gtccacaggt cgacctgcgg cttgatgttg agcttcgtcg ccccggaacg ctccagcgcc
     3061 gccatgtcga tctcgttgtc gaagtggccg atgttgccca ggatcgcgtg atccttcatc
     3121 gccttcatgt gctcgagcga gatgatgtcc ttgttgccgg tcgaggtcac gacgatgtct
     3181 gcgtcgccga tggcctgctc gaccgtcacg acgtcgaagc cctccatcag cgcctgcagc
     3241 gcgttgatcg ggtcgatctc ggtgacgacg acccgtgcgc cctggccctt cacagcctcc
     3301 gcgcagccct tgccgacgtc gccgtagccg caaatcagga ccttcttgcc gccgatcaac
     3361 gcatcggtgc cgcggttgat gccgtcgatc agcgagtgcc gggtgccgta cttgttgtcg
     3421 aacttggact tggtcaccga gtcgttgacg ttgatcgccg ggaaggccag gtcaccggcc
     3481 gcggcgaact ggtagaggcg cagcacgccg gtggtggtct cctcggtcac gcccttgacc
     3541 gactcggaga tctttgtcca cttctctttg tcggtctcga agcgattgcg cagcacgctc
     3601 aggaagacct tccactccgc ggagtcgtct tcttcggcgg gcgggaccac accggcctgt
     3661 tcgtattgcg caccacgcag caccatcatc gtggcgtcgc cgccgtcgtc gaggatcatg
     3721 ttggccggct tgtcggggtc gggccaggtc agcatctgct cggcggccca ccagtactcc
     3781 tcgagcgtct cgcccttcca ggcgaacacc gggacaccct tgggctcctc ctgggtgccg
     3841 tgcttgccga cgaccacggc cgcggccgcg tggtcctggg tcgagaagat gttgcaggac
     3901 gcccaccgga cctcggcgcc cagttcgacc agcgtttcga tcagcacggc ggtctggacg
     3961 gtcatgtgca gcgagcccga gatgcgggcc cccttcagcg gctgcacttc ggcgtattcg
     4021 cggcgcagcg acatcaggcc gggcatctca tattcggcga ggtcgagttc cttacggccg
     4081 aattccgcca acgataggtc ggcgatcttg aaatcgatgc cgttacgaac gtcggcggtc
     4141 aacgagcttt cggtcgtcgt catgcgtgtt tcatcctttg cagggctcac ggggatttaa
     4201 gcggtactta gcctaggtat gctcttccgc cactgtagtc gccggtccgt cagccccgcg
     4261 ggcgagggaa caatcacgga atgttgacga ccccatgacg ctcggcgaac ggcgtcatca
     4321 gccgtgccag gtccgccgcg acgtcgtgat cggcttccgg cggcatgggc acgtagctca
     4381 atgccagccg cacgatggcc cgcgccagca cacccgcgtc ttcctcgctg gtggacaccc
     4441 aggtctcggt caatgccaag gtcagccggg ccgacgcgcg cgtgatgatc ggcgcgctgt
     4501 cggtcgtgat gagctgcagc agatcgggtt tggcgacacc ggtcagcaac gagatcacca
     4561 acgggtcggc cgcggactcc gcgaaaaagg aacgaaagcc ctgcagcagc gcctcgtaga
     4621 tgttgccgac gttggcgtcc agcgcggcgt ggacggcgtc gaccaggcgg tcggccaggc
     4681 gcagggcgta accctgcgcc aggccctgcc gtgagccgaa ctcgttgtaa atggtctgcc
     4741 ggctgatgcc cgcggcgcgg gccacgtcgg acagcgtgat cgccgaccag tcgcgaatca
     4801 gcagcagatc ccgcatggca tccagcaccg agtcacgcag tagggcacgc gacgcctccg
     4861 cgtaagggat gcgcttcaca gccgcgaccc tatctgtttt cgcatcggca tctgttttcg
     4921 catcgatatc tcttttttca tcgatggagg tgctgatcaa ggcctggcga cctcgaccat
     4981 ctcgaagtcg gatttcgccg cgccgcagtc cgggcagctc cagtcgtcgg cgatgtcgtc
     5041 ccagcgagtg cccggcgcga tgccgtcttc cggccagccc agcgcctcgt catactcgaa
     5101 gccgcactgg atgcagcgaa acaatttgtg gtcactcatc gggtcactcc tagttcttgg
     5161 aagtcgagtt tctcgcgcac cgagcagtcc gggcagcacc agccgtcggg aatctgcgcc
     5221 caccgtgtgc ccgccggaaa gccttctcgc acatcacctt tggcctcgtc gtagacgtag
     5281 tcacagaccg ggcactgata cgcggccgtc accgttgggc ctcgtgggag gccccgtaac
     5341 gggccaacaa cttgtcccgc agccgtggct gcaggttgac ccgggtgatg tcgccgtcgt
     5401 agtgcgcgag cacccgatgg tccatcacct tgcgccacag cggcgggaag taggtcagcg
     5461 agatcatcga tgcgtagccg ctcggcaggt tgggcgcgcc cgccacgctg cgcagcgtct
     5521 ggtagcggcg ggtggggttg gcgtggtgat cgctgtgtcg ctgcaggtgg tagaggaaca
     5581 ggttggtgac cacgtggtcg gagttccagc tgtgcaccgg ggtgcaccgc tcgtagcggc
     5641 tttttccgga agcggtgtcg tgccgctgcc gcagcagccc gtagtgctcg aggtagttga
     5701 cggcctccag caggctgaag ccgaaaacgg cctggatgat cacgaacggg atcagcgccg
     5761 gaccgaaaac cgcggtcagc acaccccaca acaccaccga catcgcccag gcgttcagca
     5821 cgtcgttgga cgcatacgtc ttgggattcc aggggctggc accctgtcgg cggatacgct
     5881 gtgcctccag ccgcactgcg gagcgcaggc tgccgaacac gctgcgcggc aggaactccc
     5941 agaacgtctc accgaagcgg gaggacgccg ggtcctccgg ggtggcaacc cgcacgtggt
     6001 ggccgcggtt gtgctcgatg tagaagtgcc cgtagcagga ctgggccagg gtgatcttgg
     6061 acagccaccg ctccagcgaa tctttcttgt gccccatctc gtgggcggtg ttgatgccga
     6121 cgccgccgag aacaccgacc gacagcgcca cgcccaactt cccgacccag ctcaacgggc
     6181 cgacataacc cagccagctg agattcgtgg cggtgaacaa ataggcgccc agcgccacgc
     6241 tgaggtactg gaacggaatg tagacgtagg tgcagtaccg gtagtacttg tcgttttcca
     6301 gttgctccat cacctcgtcc ggcgggtttt gcccatcggg cccgaagccc aggtcgagga
     6361 tcggcagcag gacgtagagc aggatcggcc cgatccacag cggcacctgc gcggcgacgt
     6421 gccagcccaa ctggttcagc ccccagatga gcggcagcat cacgaacaac gccgtcggag
     6481 cgatcagtcc catcagccac agatggcgct tcttgtcccg ccagaccggc acttgggccc
     6541 cttgggcctc gcgcataacc ggcggtccga gtgtggtcat atgccaaacc tccttgtgtg
     6601 ccatcccacg atgagatttg acattaccta gctctgcgtc tcgtgtctag acaaattatg
     6661 gttgtttgta aaccagtagc cgcagtctac gtcatcgctc cgtcgcggct cccatccgcg
     6721 cggtctctgg gagctgcccg gcctgccgac gtccctgcac ggagttgcgg tagccatagc
     6781 cgccgtagat cgcggttccc accaccagcc agacggcgaa ccgtacccag gtgagcgcgg
     6841 tgaggttcag catcaaccac acgcaggcgc atatcgaggc gatcgggagc cacggcaccc
     6901 agggggcccg gaatccccgc tcgagatcgg gcctgctccg gcggagcacc atgacgccgg
     6961 ctgacaccag gacgaacgcg aacagcgttc cgacgttgac catctcctcg agcttgtcga
     7021 tcggaaacac cgacgccgtc gcggcgatca cgatcgcgac cagcacggtg attcgcaccg
     7081 gagtcccccg cgaactggtc ttggccagcg cgcgcggcaa cagcccatcg cgcgccatcg
     7141 cgaacaagac ccggcactgc ccgagcacca gcaccatcac gacggtggtc aggcccgcga
     7201 gcgccccgat ggcaatgatc ttgcttgccc attgcacgcc gttcgccgtg aacgcggtgg
     7261 ccagattcgc cggctggtgg ccggcaaccg tcttgagctg ggtgtaggag accatgccgg
     7321 acagcacgac cgacaccgcg acgtagagga cggtcacgat tgccagcgac tccaggatcc
     7381 ccctcggcac gtcccgctgg ggccgtttgg tctcctcggc catggtggcc acgatgtcga
     7441 aaccgatgaa cgcgaagaac acgatcgacg cccccgccaa cacgccgtac cagccgtaat
     7501 ggctgctgtg cgccccggtc agcagcgaca gcaccgactg gttgataccc gatgcgtcgc
     7561 ccccggattc gggcttcgga atgaacggcg agtagttgga gcctttgatg tagaacagcc
     7621 ccaccaccac caccagcacc accaccgaca ccttgatggc ggtgatcacc gcggaaaacc
     7681 tcgacgagag cttggtcccc agggcgacca gcgtcgccac cacggcgacg atcagcagcg
     7741 cgccccagtc gagctggacc gatccgaagg tgacggtgcc accggcgaat ccgaagaccg
     7801 tacccaggta gctcgaccag cccttcgata cgacggcggc gccgatcgcc aattccagca
     7861 ccaggttcca gccgatgatc caggcaagga actcgccgaa ggcggcgtag gaaaaggtgt
     7921 acgcgctgcc cgccaccggc agcatcgagg cgaattccgc gtagcaaagc gcggccagcg
     7981 cacaggtgat cgccgcgatc acgaacgaca cccagatggc cggcccggtg atgtcgccgg
     8041 cggtcgacgc ggtgaccgtg aagatgccgg caccgatcac caccgcgacc ccgaaaaccg
     8101 ccaagtcccg ccaggtcaac tccttgcgca gccgggtgtc tggctcgtcg gtgtcggcga
     8161 tcgattgttc gaccgatttc gtgcgccacc gattcgccat ccacccaccc tttcccaacc
     8221 tgctgggacc cgctggaccg tcacagtact gggtagtctg cggacatgcc gggcaccgga
     8281 atcagcacca gggaccacgc ggtggtgatc ggcgcaagca tcgccggttt gtgcgccgcg
     8341 cgggtgcttt cggattgcta ttcacgggtg accgtctacg agcgcgacca gctgccgagc
     8401 gcgccggcga accgcgcgac ggtcccgtag gaccggcatc tgcacatgct gatggcgcgg
     8461 ggggcaatcg agttcgacgg cctgttcccg ggcctgctga aggacatggt ggccgccggc
     8521 gtgccgatcc tggagaaccg gccggactgc atctacctcg gcgctgcggg ccacgtcctg
     8581 gggaccggcc aaaccctgcg cgacgagttc accgcctacg tgcccagccg gccgcatctg
     8641 gagtggcaac tgcgcacgcg ggtccgagag atcgacaacg tcgagatcgt gcggcgctcc
     8701 gtcggccggc cgcggttcga ccctgcgcac cagcgggtga ccggcgtgca gctggatccg
     8761 ggcgacggcg acggcgacaa cggggaccgg gagttcgtgg gcgccgacct cgtcgtcgac
     8821 gccgcgggcc ggggcacccg cctgccggtg tggttggccc agtggggata tgcgcgcccg
     8881 accgaagaaa ccatcgacat cggcatcaac tacgcgaccc atcagttccg catgcccgag
     8941 ggcctgatcg cggagaaggt ggtggtcgcc ggcgcctccc atgaccagtc gctcgggctg
     9001 ggcatgctgt gctacgagga cggtggctgg gtgctcacca ccttcggcgt ggccggcgcc
     9061 aaaccgccga cgacgttccc cgggatgctg gcgctggccg acaagctgct tcccgtgcat
     9121 ttcaccgccg cgctggcaca ggctgaaccc gtcggggaca cggcgctgca tgcctttccg
     9181 accagcaggt ggcgtcgcta cgacaagctg gaccgtttcc cggccgggat cgtcccgttc
     9241 ggtgatgccg tggcgagctt caaccccacc ttcgggcaag gcatgaccat gacgtcgctg
     9301 caggccggga atctgcgccg ggctttgcaa tccccggacg accagcttgc cgcggaactg
     9361 agccgcgcta ccgcgaagac gacctacccg gtgtggatga tgaatgccat cggtgacatc
     9421 accttccacc atgccggcat ccacggcccg gtcccctggt ggtggcgacc gtcgggtgcg
     9481 ctgttcgacc aattcctcgg ggcggccgaa accgaacccg ttctcgccga atggtttttg
     9541 cgccggttct cgctgctcga cagcctgtac atggtgccgc ccccacgggt gatcggccgc
     9601 gccatcagac acaacatgcg gctgtggctc cgcgagcgcc gggagtcagg gcgggccgtc
     9661 acaacccgac ggtcgccctg aacagcttgg cgggctcccg ggcgacgagc cggatgggac
     9721 catcgtcggc ggccacccag gcggccatcc cgtgttccag cgtgagcgac ccggacttgc
     9781 cgtgcaccgt cgtgcagccc tcggtgcaca acaggatctg cggcccctcg tggtgcgacg
     9841 gcgcgtcgac ctcgtggccg acatactcgc cgtcgagcgc cagcaatgcg accgcgaact
     9901 cgtcggtcgg ggtctcgtag agccgctgga gtccctctcg gcggacgggc gcccgcagct
     9961 gtgcctcggt cgtcggggcg aagtccagca cccgcagcag ctcgggcaca tcgacgtgct
    10021 tgggggtgag ccccccgcgt aacacgttgt ccgagttggc catcacctcg agcgcgaacc
    10081 ctcgcagata ggtgtggagg ttgccggccg ccaggaagat cgcctcgccc ggggccaggc
    10141 tgatgcggtt gagcagcagc gcggccagca ccccggcgtc gccgggatag cgttcgccga
    10201 gttccagcac cgttttcgct tcgggcgcga attcccttgc ccccgagctg acgtaggcga
    10261 tggcgccgtc cagcacggcc gacaccagca cgtcgatgtc gggctggggc gcggtgatcc
    10321 aggtggtgaa cagcgcacgc aggccgcccg cgtcggaccg atcgttcagc aattcgatga
    10381 aggggtcgag gtcggacacg gccaatgccc gcagcagttc gatggtccgc gacactggac
    10441 gaaagccggc aagcgcctcg aacgagagca gcgccaccag caactccggc ttgtgcgagg
    10501 tgtcgcggta gttgcggacc ggcgaggaca ccggaatgcc cattcgctcc tcacgccggt
    10561 aaccctcgac cgcctgcgcg gcactgggat gagcctgcag tgacagcggc tcgtcggcgg
    10621 ccagcacctt gaccagatac ggcagcacgt ccccgaaccg ggcgcgcgac ccggcgccga
    10681 gctgtccctc cggatcggcg accaccgcat ccagcagtga gatctcgccc ttgtccgttt
    10741 ccagccaggc cggatcaccc ggattcgcgc cgaaccaaag ttcggcctcc gggtgcgccg
    10801 ccggaaccgg acgcccggtg aattcggcga tcgcggtgcg cgatccccac gcgtatgtcc
    10861 gtaaggcccc gcgaagcagt tccacttgtc tatctatccc cgcaccagtc gcatgtaaac
    10921 cgcggccatc tcgagccgaa cggccaatac tgccagttgc tgctcgacgc gcccggcacc
    10981 ggtcggcgcg cgcacaccgg atgcggccac cccgtcgggt ccgaccgggc cggcggcggc
    11041 gtcgggcaca tccggcacgt ccccggccgt caccaggtcg acgccgtcga accccgcgat
    11101 cccagccgcc accacagtct gctcggcggc cagcgccagc gccagcacac gcagccgccg
    11161 cggcagtggt ccgtcgatct cttcgtcgtg aaagagagcg tccaccgagg aggcggtgtc
    11221 gctgaactca tcggccagcc cgccgcgtag cgccaccagc gcgtcggcca gcccggtggc
    11281 ggcgaccacc tcgtgcgcga gccgcagcag caccgaactg ccatgacggg ccagcgccag
    11341 tgtcgcggcg ttgccgccgg ccagcgccac ccggcgaccc gccatccgcg cggcaatggt
    11401 cttggccggg ttggtgaaca gctcgcggcc ggtgctgttg cgcagcgcct cggcatccag
    11461 ctcgtcggcg agcaaagcca gatcgatgct cggtctgggg tcgacgattt tcagaaccgc
    11521 cagtccggcg gccaggtagc ggcacaatcc gaactcgttg ggaatccaca tccgcggcgc
    11581 cagcaccgcg gcgcggccgg cggtggcgtc ccgcagcggg ccctcatagg gcgccaccac
    11641 aacgacccgc gcaccgcggc gcaccccggt cgcggcggcg gcgaccagcg cgggatcgcc
    11701 cggatcgtcg cccgcgacga tcaacacatc aagcggacct acccagggcg gagcctcagc
    11761 gacgaccgcg atcggctcgg aggccgtacc gcccaacgtc gcggccagca tggcaccggc
    11821 agtctcggcg gtaccacggc cggccaccca gatcaggctg cgcggacggt cttgcgtccg
    11881 cagcgcatcc agtgcgccct cgtcggctgc ggccgcgatc gcgcggacct gtgccccggc
    11941 cgacgacgcg gcccgtagca gcccctcccg gtcggcggca agcagccccc cggtgtcttc
    12001 cagatcgatc gcccgggtcg cgttcacgat cccgcctcgt cgcgcgccgc ctgggtgacg
    12061 gtcgcggcga tttcagccct gacctgcgca accaccgcgt cgacgtcgtc ggtgctgcgc
    12121 gcctccacat tgagccgcag caacggctcg gtgttggaac tgcgcaggtt taaccagctg
    12181 ccgtcgccca aatcgaccgt caccccgtcc agatgatcga tggactgaat ccggcc
//
LOCUS       DASSGQ010000133         3623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_678430,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000133 DASSGQ010000000
VERSION     DASSGQ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678430"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFI55_06255"
     CDS             complement(<1..206)
                     /locus_tag="VFI55_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET7073669.1"
                     /translation="MGADSGTVRSRLWRDGALAAEDFPFELLSDYLDEDGCLVWVDIR
                     GPDPQHLNNLAEELTLDPHAVEDSL"
     gene            complement(379..543)
                     /locus_tag="VFI55_06260"
     CDS             complement(379..543)
                     /locus_tag="VFI55_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073670.1"
                     /translation="MNEQRIEANRLLGGEVGEQLLRELSHPVDERFSSGAAACGESRS
                     RGVGDVGGDK"
     gene            1139..1585
                     /locus_tag="VFI55_06265"
     CDS             1139..1585
                     /locus_tag="VFI55_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073671.1"
                     /translation="MIDTAACPPVLGGPDDLDAVVCGFVAKAKSELLVAVRELDSLTI
                     TTALLAAKKVQVRVRVILEGDYLVEPKPLPDPWTSGGDYEANRSIHSALLRAGIDVIT
                     DLNPAIFHQVPRPRRRRERRRRAHWLDQLHADRHRHQPGHEHRQGR"
     gene            complement(2266..>3623)
                     /locus_tag="VFI55_06270"
     CDS             complement(2266..>3623)
                     /locus_tag="VFI55_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HET7073672.1"
                     /translation="DRDNRITVGRDTGGTINVNNGDVRIHGTRATVDNVGVIRIFGSG
                     GNDALAIDEANGALPAALMFGGSGNDRILGGSGDDRLFGGSGDDTLIGARGDDQVFGN
                     SGNDRMIWNPGEGTDINEGGDGVDTVEVNGGNVAETFTATPNGTRVRFDRVTPLPFSI
                     DIGTSENLELNANGGDDQFSAGNVASLISITVNGGAGNDTLLGGNGADTLNGDDGNDF
                     VDGNGGADVSTLGAGDDTFQWDPGDGSDVVDGQDGHDTMLFNGADTAESMAVSANGHR
                     VLFTRNLGNIRMDLDGIEQVDTNALGGADEITVNDVTGTDLTAVNLNLASRVGGGDGA
                     ADSVIVDATNDNDVVKVTGTATDGVTVSGLNATTRISGTDAGDTLTVNGQAGDDAIDA
                     STLAAGAVALTADGGDGDDVLQGSAGEDTLLGGAGDDVLNGGPGLDVLDGGTGLNVIT
                     Q"
BASE COUNT          592 a         1206 c         1159 g          666 t
ORIGIN      
        1 agggaatcct ccacggcgtg tgggtcgaga gtcagctcct cagcgagatt gttgaggtgc
       61 tgcggatccg gaccgcggat atcaacccac accaaacagc catcctcgtc gaggtagtcg
      121 gagagcagct cgaacgggaa atcctcggct gccaaggccc cgtcacgcca cagccggcta
      181 cggaccgttc cgctgtcagc acccacacgc acagtgtgcc cgctcgccgg tgctgacatg
      241 cggcttgccc tggcagccag acgatccgcc cgatccacag cgtcgagcac gcgccggcgc
      301 ggccattctg cagaactgat ccgatctggc agcacatcgg tggcatacat cgacagtgac
      361 acgtccaagg ccagccacct acttgtcgcc gcctacatcg cccacgcctc tcgatcggct
      421 ttcaccgcac gcggccgcgc cgctgctgaa gcgctcgtcg acggggtgcg aaagctcgcg
      481 cagcagctgt tcgccgactt caccaccgag caatcggttt gcttcaattc tctgctcgtt
      541 cacgtgctcg agcgcctttg gcagcctcat gatggaggac tcacgcggac gagatcaggg
      601 acgtcggccg tgtcgcagcc ctcctggcca gcgcattact caagagcagg tgcctgctca
      661 aaccccggcg gccacctctc ctcaccaccc gcaggtgcca ccgcgaggcg cagccccgcc
      721 agcaagagac gacttcgctc ctgaccgcgg gaacggacat tcggccttgt gctgcaacgg
      781 gtggaacgct gagcatgggt cccctcgccg gccgagtcct caatgcagca gcgagggtcg
      841 tcgaactcac cctggtcggc gcgtcgaccg cgagcagcca tgcacgtgac ggcatgcgta
      901 tgcaggtcac accagcctgt catcggcaga gccgagttga tccagctgca gaaaagaccc
      961 ctcgtggtcg agcgcaatgt gccggaaata acaccaggcg ctcatgccgc atcgcgcggc
     1021 ttcatgtgac gcactcatct gccgcagacg gcgcgcactt gacagcacgg gatccggagt
     1081 tctgtgacgc gaacgactcg tatggatttc cgagccgccc ttggagtgtg gaatagcgat
     1141 gatcgacaca gccgcctgtc cgccggtgct cggcggaccg gacgacctgg acgcggttgt
     1201 gtgcggcttc gtcgccaagg ccaagtccga gttgctcgtc gcggtacggg aactcgattc
     1261 gctgacgatc accaccgcgc tgcttgccgc caagaaggtg caggttcggg tgcgggtcat
     1321 cctcgagggt gattacctcg tcgagccgaa accgttgccc gacccctgga cgagcggcgg
     1381 ggactacgag gccaaccgca gcatccactc ggcgttgctt cgggccggca tcgacgtgat
     1441 caccgatctc aacccggcga tctttcacca agttcctcgt ccgcgacgac ggcgggaacg
     1501 gcgccgccgt gctcactggc tcgaccaact tcacgctgac cgacaccggc accaacccgg
     1561 ccacgaacac cgtcaaggtc ggtaacaatc tcaaccacat tgtggtgctg cacggccaga
     1621 gggcggtcaa ccagtaccgg gcggagttcg accggctccg cagcggcacc ttcggtgagc
     1681 tgcacgagcg atctgcagcg gtcgtgtcgg cgatccctcc accgtcgaca ggctgaacct
     1741 gggtgcacgt accagtggcg acagaacgcg tccgcgatcg cctcctcgga ctgctgccag
     1801 ctcagcgccg caagctcgtc gagtgtgtgc ttcttgctga gtcggtatgc ccggatgagc
     1861 gcgtcgagga gcgcggcaat gagcaccatc gcgatcgcgg cgccccggac gaggacttat
     1921 agctgactcg gcagcgcctg cgcatcatcg caactttact cacccaagca gcctccgaca
     1981 ccggcccgga ccgggctgta ttggaccttc gtgacggtcc tatggactgt caagttcggg
     2041 ggcggtggcg gggcaggccg ttacggctga ggtttggctc agcgttggtg gtgtcgtcgc
     2101 aacgatgcgc ggcgtcgcgc atgcgtttgt ttcgtcaagg atcgatgctc aagtttcatg
     2161 ggctactcgc gatttgcggc agcagttcgt tgtgtgtgct ttggcgaacg atttctcatg
     2221 caggtgggcc ggcccgaacg gaccggccca ctgacgatgt gggatttact gggtgatgac
     2281 gttcaggccg gttccgccgt ccagcacgtc taggccgggt ccgccgttga gcacgtcgtc
     2341 gccggcgccg ccaagcagcg tgtcctcgcc ggcgctgcct tgcagcacgt cgtcgccgtc
     2401 gccgccgtcg gcggtaagtg cgaccgcgcc agcggccaga gtggaggcgt cgatcgcatc
     2461 gtcgccggcc tgtccgttga cggtcaacgt gtcgccggca tcggtgccgc tgatgcgagt
     2521 cgtcgcgttc aggccggaga cggtgacgcc gtctgtggct gtgccggtga ccttgacgac
     2581 gtcgttgtcg ttggtggcgt cgacgatgac gctgtcagcg gccccgtcgc caccgccgac
     2641 tcggctggcg aggttcaggt tgacagcggt gaggtcggtc ccggtcacgt cgttgacggt
     2701 gatctcgtca gcgccgccga gcgcgttcgt gtcgacctgc tcgatgccgt caagatccat
     2761 cctgatgttg ccgaggttgc gggtgaacag cacccggtga ccgttggcgg acactgccat
     2821 gctctccgca gtgtctgcgc cattgaacag catcgtgtcg tgaccgtcct ggccgtcgac
     2881 gacgtcgctg ccgtcgcccg gatcccactg gaacgtgtcg tcgcccgcgc ccagcgtgga
     2941 cacatcggcg ccgccgttgc cgtccacgaa gtcgttgccg tcgtcgccgt tgagggtgtc
     3001 agcgccgttc ccgcccagca gcgtgtcgtt gcccgcgcca ccgttcacgg tgatcgaaat
     3061 cagcgaagcg acattgccgg cggagaattg atcgtcgccg ccgtttgcgt tgagttccag
     3121 gttctccgac gtgccgatat cgattgagaa cggcaacggg gtaacccggt cgaacctcac
     3181 acgggtgcca ttcggcgtcg cagtgaaggt ctctgcgacg tttccgccgt tgacctcgac
     3241 ggtgtcgacg ccgtcgcctc cctcattgat gtcggtgccc tcgcccgggt tccagatcat
     3301 gcgatcgttg ccgctgttgc cgaatacctg gtcgtcgccg cgggcgccga tcagcgtgtc
     3361 gtcgccggag ccgccgaaca gccggtcgtc gccggagccg ccaaggatcc ggtcattacc
     3421 ggatccaccg aacatgagcg cagccggcaa ggcgccgttc gcctcgtcga tggcgagcgc
     3481 gtcgttgccg ccggaaccga agatacggat gacgccgacg ttgtcgaccg tcgcccgggt
     3541 gccgtgaatc cggacgtcac cgttattgac gttgatcgtg ccgccggtgt cgcggcccac
     3601 ggtgatcctg ttatcgcggt cgt
//
LOCUS       DASSGQ010000134         3443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_313587,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000134 DASSGQ010000000
VERSION     DASSGQ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313587"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="VFI55_06275"
     CDS             <1..1211
                     /locus_tag="VFI55_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7073673.1"
                     /translation="ARCELAAERARAALDSPDLSDFHAMMASVALIMAMGALGEVDEL
                     ADVAEQTLRRATTSFQASHLRFWFGAVYGRACRLTGRIDDFVSTAKQVADSARDVPGL
                     AYANLAFLLGNAELARGAVADSARLVHEAVAGVQINSITTGLRPASYFALAEAHAKLG
                     QPGEANDAVARARAAVPPDFLFMDTALSLAGGWAMVASGCLNEAVASVQQAARLARDR
                     GQPTHELACIQAAAQWGDASHAGRARELADALSLPLADAVASHTRALLAGDGEGLLAA
                     SAAYRQIGDRVAAADAAAQASVAFTDSQRHKRGLYAAALARELADECGGLRTPAMRTP
                     TGLKLSGRQRDVVELVLAGLSNRQIADRLVMSVRTVEGHIYRACQRVGAQSREELASI
                     IQSGPGSGAG"
     gene            complement(1217..2458)
                     /locus_tag="VFI55_06280"
     CDS             complement(1217..2458)
                     /locus_tag="VFI55_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET7073674.1"
                     /translation="MLLTVALVLLAVVLAFAVARPGGWPEALAAVPAAAILIAIGAIS
                     VHQAAEEVTGLSGVVAFLGAVLVLAKLCDDEGLFEAAGAAIARGRVGSGGLLGRVFVI
                     SAVITAVLSLDAAVVLLTPVVLAAVRRLRTRVRPYAYATAHLANGASLLLPVSNLTNL
                     LAFHYARISFAKFTLVMALPWLTAVAILYGIFRWFFARDLRVQPDPEQLGPASRPPVF
                     VLVVVGLTLVGFAIAQSVGVAPAWVALIGASLLAVRSLSRGHTSVAEIVRSAHVSFLL
                     FVLALGVVVQAVMLNGMDRAMSAVLPSGAGLPALLTSAAIAAVLANIVNNLPATLVLL
                     PLVAPGGPAAILAVLIGVNIGPNLTYVGSLSNLLWRRVLRQHGVDAGVGEYTRLGLCT
                     VPPALVVAVLALWASVRLLGI"
     gene            complement(2472..3392)
                     /locus_tag="VFI55_06285"
     CDS             complement(2472..3392)
                     /locus_tag="VFI55_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7073675.1"
                     /translation="MRLKLGRPDIARYSDRFDVPAAEPDSPLSATWMGVATVLLDDGS
                     SALMTDGFFSRPGLAQVIAGKLASSPARVDGCLTRAKVSRLEAVIPVHTHYDHTMDSA
                     LVADRTGARLVGGESAANVGRGYGLAEDRIVVAVSGAPIRLGAYDVTLIESHHCPPDR
                     FPGVIGAPVVTPVKVSAYRCGEAWSTLVHHRPSGRRLLIQGSAGFVKGALAGHRADAV
                     YLGIGQLGLQPRSYLVDYWNETVREVGARRVVLIHWDDFCRPLSKPLRALPYAGDDLD
                     VSMRILDELAAHDGVALHLPTVWRRENPWT"
BASE COUNT          593 a         1206 c         1185 g          459 t
ORIGIN      
        1 cggcgcgctg cgagctcgcg gccgaaaggg cccgggccgc actggattcc cccgatttgt
       61 cggactttca cgcgatgatg gcgtcggttg cgctgatcat ggccatgggc gcgctggggg
      121 aggtcgatga gcttgccgac gtggccgagc agacgctgcg gcgtgcgacg acatcgtttc
      181 aggcgtccca tctgcggttc tggttcggcg ccgtgtacgg ccgcgcctgc cggctgaccg
      241 ggcgtatcga cgatttcgtc agcacggcaa agcaagtggc cgactcggcc cgagacgttc
      301 ccggtctggc ctatgcgaac ctggccttcc tgttggggaa cgccgaactg gctcgcggcg
      361 ccgtcgccga ttcggcccgg ctggtgcacg aagcagttgc gggggtccaa atcaactcca
      421 tcaccacggg tttgcggccg gcgagctact tcgcgctggc ggaggcacac gccaagctcg
      481 gacagcccgg cgaggcgaat gacgcggtcg ccagggcgcg ggcggccgtg ccgcccgact
      541 ttctgttcat ggataccgcg ttgtcgttgg ccggcgggtg ggccatggtg gcaagcggct
      601 gcttgaacga ggcagtggcg agtgtgcagc aggccgcacg actcgcccgc gaccgcggcc
      661 agccgaccca cgagctggcc tgcatccagg cggcggccca atggggcgat gcctcgcatg
      721 cgggtcgcgc gcgcgaactg gccgacgcgt tgtcgctgcc gctcgccgat gcagtcgcgt
      781 cgcacacccg ggctctgctg gccggggatg gcgaaggcct gctggcggct tcggcggcgt
      841 accggcagat cggtgatcgg gtcgcggccg ccgacgcggc ggcgcaggcc tccgtcgcgt
      901 tcaccgacag ccagcggcat aagcgcgggc tgtatgccgc ggcgctggcc agggaattgg
      961 ccgatgaatg cggcggattg cgcacgccgg cgatgcggac tccgacgggt ctgaagttgt
     1021 cgggtcggca gcgcgatgtc gtcgaactcg tcttggcggg cctgtccaac cggcagatcg
     1081 ctgatcggct ggtgatgtcg gtgcgcaccg tggagggcca catctatcgg gcctgccagc
     1141 gggtcggcgc ccagtcgcgg gaggaattgg cgtcgatcat ccagtcggga cccggcagcg
     1201 gcgcaggctg acgcgcctag atacccagca gtcgcacgct cgcccacagc gcaagcaccg
     1261 ccaccaccag cgccgggggt acggtacaca gcccgaggcg ggtgtactcg ccgacgccgg
     1321 cgtcgacacc gtgctggcgc agcacgcgcc gccagagcag gttcgacagc gagccgacgt
     1381 aagtcagatt cgggccgatg ttgaccccga tcagcaccgc caggatggcc gccgggccgc
     1441 cgggcgcgac cagcggcagc agcaccagcg tggcgggcag gttgttgacg atgttggcca
     1501 acacggcggc gatcgcggcg ctcgtcagca atgccggcag tcccgctccg gacggcagca
     1561 ccgccgacat cgccctgtcc atcccgttga gcatgaccgc ctgcaccacc acgcccagcg
     1621 ccagcacgaa aagcaggaaa gacacgtgcg ccgagcgcac gatctccgcc accgaggtgt
     1681 gcccgcgact caggctgcgc accgccagga gcgaagcgcc gatcagcgcc acccaggccg
     1741 gcgcgacgcc cacggactgg gcgatcgcga acccgaccag cgtcagccca acgactacca
     1801 acacgaacac cggcgggcgc gacgccggcc cgagctgttc gggatccggc tggacgcgca
     1861 ggtccctggc gaagaaccac cggaagatcc cgtagagaat ggccaccgcg gtcagccatg
     1921 gcagcgccat caccagggtg aacttggcga atgagattct ggcgtaatga aaggccagga
     1981 ggttggtcag gttcgacacc ggcagcagca gcgaggcgcc gttggccagg tgagcggtgg
     2041 cgtaggcgta aggccgcacc cgggtgcgca accggcggac ggcggccagc accacggggg
     2101 tcagcaacac gacggcggcg tccaggctga gcaccgcggt gatcacggcg gagatgacga
     2161 acacccgccc gagcagaccg cccgatccga cccgcccccg cgcgatcgcg gcgcccgccg
     2221 cctcgaacag tccttcgtca tcgcacagct tggccagcac cagcaccgcg cccaggaacg
     2281 cgaccactcc cgatagcccg gtcacctcct cggcggcctg atgcaccgat atcgcaccga
     2341 tcgcgatgag gatcgccgct gccggaacag ccgccagcgc ctccggccag ccacccgggc
     2401 gcgcaaccgc gaacgcgagc actaccgcaa gcagcaccag cgcgacggtc aatagcaacg
     2461 tctagaccgc ttcacgtcca agggttttcg cgccgccaca cggtgggcag gtgcagcgcg
     2521 acaccgtcgt gtgcggccag ctcgtcgaga atgcgcatgg acacgtccaa gtcatcgccc
     2581 gcataaggta aggcccgcaa cggctttgac aaagggcggc agaagtcgtc ccagtggatc
     2641 aggacgaccc ggcgcgcgcc cacctcgcgg acggtctcgt tccagtagtc gaccagatac
     2701 gaccgcggtt gcaatccgag ctgtccgata ccgaggtaga cggcatcggc gcggtggcct
     2761 gccagcgcgc ctttcacgaa acccgcgctg ccctggatca gcagccgtcg gcccgacggc
     2821 cggtgatgca ccagcgtcga ccacgcctca ccgcaccgat atgccgacac cttcaccggc
     2881 gtcaccacgg gtgcgccgat tacacccgga aaccggtcgg gcgggcaatg atgcgactcg
     2941 atcagcgtga cgtcataggc gcccaaccga attggtgcac cggagaccgc gacgacgatc
     3001 cggtcctcgg ccagcccgta tccgcgcccg acgttggccg cggactcgcc accgaccagc
     3061 cgggcgccgg tgcggtcggc gaccagcgcg gagtccatcg tgtggtcgta gtgggtgtgc
     3121 accgggatga cagcttcgag ccgcgacacc ttggcccggg tcaggcagcc gtcgacgcgc
     3181 gccggcgacg acgccaactt gccggctatc acctgcgcca ggccgggtcg ggagaagaac
     3241 ccgtccgtca tcagcgcgga cgacccatcg tcgagcagca ccgtcgccac gcccatccag
     3301 gtcgccgaga gcggcgaatc cggttcggcc gcaggcacgt cgaaccggtc cgaatagcgt
     3361 gcgatgtcgg gtcgaccgag cttcagccgc atcagcaaaa ccccctgata tcagtgccca
     3421 tggcggtgag tcggtcgcgc acc
//
LOCUS       DASSGQ010000135         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_617524,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000135 DASSGQ010000000
VERSION     DASSGQ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617524"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1549)
                     /locus_tag="VFI55_06290"
     CDS             complement(56..1549)
                     /locus_tag="VFI55_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HET7073676.1"
                     /translation="MSPGPDAGATNKLASKVARQIEADIVRRGWTVGESLGSEQALQQ
                     RFRVSRSVLREAVRLVEHHQVARMRRGPGGGLLICRPDAGPATRAVVIYLEYSGTTLR
                     DLLNARLVLEPLAASLAAERIDEAGIDRLRAVLRAEEHWRPGVAALRDEFHIALAEQS
                     KSPVLQLFIDILMRLTARYALQSRADSATEAVEAVDQMHRDHSDIVAAVTAGDSARAT
                     TLSERHVEAVTAWLQRHHPGDEVTGKAGKGRHSPAAPRRLNVDAPPGKLAEMLAATIA
                     DDIAADGWQVGSVFGTEATLLARYRVSRAVLREAVRILEYHSVARMRRGPGGGLIVTK
                     PQAQASIDTIALYLQYRKPSGEDLRRVRDAIEIDNVAKVVKRRGESEVAAFLDTHGDT
                     HASVLDGNPRDTAGDVRQAAVEEFRFHLGLAQLAGNALLDLFLRIIIELFRRHWTSTG
                     QPLPTWADVVAVEHAHLRILRAIEAGDDSLARYRLRRHLDAAASWWL"
     gene            complement(1583..>2026)
                     /locus_tag="VFI55_06295"
     CDS             complement(1583..>2026)
                     /locus_tag="VFI55_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET7073677.1"
                     /translation="AVEAIGGSDGAGGWFHRDDYPKMAMSDAAAQMWSRTQLTPAELD
                     VAQLYDGFTFLTVAWLEALGICGDGEAGPFVEGGARIARDGELPLNTYGGQLSAGRLH
                     GYWALHEGCLQLRGEAGERQVAQRPEVAVVSVGGGPVAGCMLLTC"
BASE COUNT          315 a          707 c          683 g          321 t
ORIGIN      
        1 ctttgatacg cgcacgaaac cgatcacatc cacgcggatt gcgacgaccg cgtccctaga
       61 gccaccagga ggcggcggcg tccagatgac gacgcaggcg ataccgggcc aggctgtcgt
      121 cgccggcctc gatggcccgc agaatgcgca aatgagcgtg ctcgacggcc acgacgtcgg
      181 cccacgtcgg cagcggctgt ccggtgctgg tccagtgccg gcggaacaac tcgatgatga
      241 tccgcaggaa gaggtcgagc agcgcgttgc cggccagttg cgccagtccg aggtggaacc
      301 ggaactcctc gacggcggcc tgacggacgt cgccggcggt gtctcggggg tttccatcca
      361 gcacggacgc atgggtgtcc ccatgggtgt ccaggaaggc cgccacctcg gattcaccgc
      421 gccgcttgac gactttggcg acgttgtcga tctcgatagc gtcccggaca cggcgcaagt
      481 cttcgccact cggcttgcgg tactgcaggt acagggcgat ggtgtcaatg ctggcctgcg
      541 cctgaggttt tgtgacgatc agcccgccgc cgggtccacg acgcatgcgc gcgacggagt
      601 gatattcgag tatgcgcacc gcttcgcgga gtaccgcgcg gctcacccgg tagcgcgcca
      661 gcaacgtcgc ctcggttccg aagaccgaac cgacctgcca gccgtcggcg gcgatgtcgt
      721 cggcgatggt cgcggccagc atctcggcga gtttgccggg gggcgcgtcg acattgagtc
      781 gccgcggggc agcggggctg tgccggcctt ttccagcctt tccagtgacc tcgtcgccag
      841 ggtgatgccg ctgcaaccag gcggtcacgg cttcgacgtg tctttcgctg agcgtcgtcg
      901 cccgcgccga atcacccgcg gtgacggcgg caacgatgtc ggagtggtca cgatgcatct
      961 ggtcgacggc ctcgacggcc tcggtcgccg agtcggcccg tgactgcagg gcgtatcgcg
     1021 cggtgagcct catcaggatg tcgatgaaca gttgcaggac cgggcttttc gactgctccg
     1081 ccagcgcgat gtggaactcg tcgcgcagcg ccgcgacccc cggccgccag tgctcctcgg
     1141 cgcgcaacac cgctcgcagc cggtcgatgc cggcttcgtc gatcctttcc gcggcaagag
     1201 aggccgccag cggctcgagc accaggcgcg cgttgagtaa gtcacgcagc gtcgtgcccg
     1261 agtactcgag atagatcacg acggcgcggg tcgcgggtcc ggcatcgggc cggcagatca
     1321 gcagcccgcc gcccggtccg cgccgcatcc gggcaacctg atggtgctcg accagtcgaa
     1381 cggcctcccg cagcaccgat cgactcaccc gaaagcgttg ctgcagagcc tgttcggagc
     1441 ccagcgattc gccgacggtc cagccgcggc gaacgatgtc cgcctcgatc tggcgggcaa
     1501 ccttggaagc cagcttgttc gtcgcgccgg catccggccc ggggctcatc tgaggctcaa
     1561 tgatgggctc ccgcaggata tctcagcacg tcagcagcat gcatccggcg acgggtccgc
     1621 cgcccacgga aaccaccgcg acctctgggc gttgggccac ctggcgctcc cccgcttcac
     1681 cgcgcaactg caggcaccct tcgtgcagcg cccaatagcc gtgcaggcgc ccggccgata
     1741 gttggccgcc gtaggtgttc agcggcagtt cgccgtcgcg ggcgatccgc gcaccgccct
     1801 cgacgaacgg gccggcctcg ccgtcgccgc atatgccgag ggcttccagc cacgcgacgg
     1861 tgaggaacgt gaagccgtcg tacaactggg cgacgtcgag ttcggcgggc gtcaactgcg
     1921 tgcgcgacca catctgcgcg gccgcatccg acatcgccat cttcgggtag tcgtcgcggt
     1981 ggaaccagcc gccggcgccg tcggacccgc cgatcgcctc caccgc
//
LOCUS       DASSGQ010000136         7125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_222045,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000136 DASSGQ010000000
VERSION     DASSGQ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7125
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222045"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFI55_06300"
     CDS             complement(<1..401)
                     /locus_tag="VFI55_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HET7073678.1"
                     /translation="MTDRSMPVPEGLAGMRVDAGLARLLGLSRSAVAALAEDGGVELD
                     GVAAGKSDRLTSGAWLQVRLPDAPAPLENTPVDIEGMTILYSDDDIVAVDKPAAVAAH
                     ASVGWTGPTVLGGLAAAGYRITTSGVPERQG"
     gene            complement(398..1066)
                     /gene="lspA"
                     /locus_tag="VFI55_06305"
     CDS             complement(398..1066)
                     /gene="lspA"
                     /locus_tag="VFI55_06305"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015289949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET7073679.1"
                     /translation="MRDEPTGPAEPVTAAEPAGDADEAAKPAAAAPQGSRNPPRRLRL
                     LLSVAAVVLTLDIVTKVLAVRLLPPGQPVSIVGDTVTWTLVRNSGAAFSMATGYTWLL
                     TLIATGVVVGIFWMGRRLVSPWWAVGLGMILGGAMGNLVDRFFRAPGPLRGHVVDFFS
                     IGWWPVFNVADPSVVGGAILLLVLSIFGYDFDTVGRRNAHGDTVGRRNADADTVGRRK
                     ADQR"
     gene            1137..2084
                     /locus_tag="VFI55_06310"
     CDS             1137..2084
                     /locus_tag="VFI55_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET7073680.1"
                     /translation="MARLTVIATGGTISTSTGPDGVRRPAYSGAELMAPLGEGLDVAD
                     VDVVDLLALDSSQLTPADWDRIRDAVQATIDDGADGVVIAHGTDTMEESALWLDLTHA
                     GDVPVVLTGAMRSADAPDADGPANLRDALAVAASPAARGLGALVCFAGRVLQPLGLQK
                     VATQDLSGFDGELIGTVAGGVTLTGAKTRPYLGDLRAADAPRVDIVAAYLGSDSVALD
                     AFVIAGARAVVLEALGSGNAGTAVVDGVRRHCRKGIVVAVSTRVPGGRVSAGYGPGHD
                     MAAAGAVMVPRLRPPQARVLIMAALAAGLPIAEVIDRLG"
     gene            complement(2049..3443)
                     /locus_tag="VFI55_06315"
     CDS             complement(2049..3443)
                     /locus_tag="VFI55_06315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET7073681.1"
                     /translation="MLHLDMDAFFASVEQLTRPTLRGRPVLVGGLGGRGVVAGASYEA
                     RVFGARSAMPMHQARRLIGVTAVVLPPRGVVYGVASRRVFDTVRSLVPVVEQLSFDEG
                     FGEPAQLAGASADDVAVFCEGLRRRVREETGLVASVGAGSGKQIAKIASALAKPDGVR
                     VVRRAEEHALLGGLPVRRLWGIGPVAGEKLNRLGIETIGDLAALTDAEAANILGATVG
                     PALHRLARGIDDRPVAERAEAKQISAESTFAVDLTSLEQLREAVEPIAEHAHQRLLRD
                     GRGARTVTVKLKKSDMSTLTRSATLPYPTTEAGALIAVARRLLLDPREIGPIRLLGVG
                     FSGLSEVRQESLFPDLELPGETSDEHPVQTAATATEAMFAPPSEAAPWRVGDDVTHRE
                     LGHGWVQGAGHGVVTVRFETRSSGPGQAHTFPADTADLAGANPVDSLDWPDYVGVLQA
                     NALSAQAVDDLGDG"
     gene            3493..4062
                     /locus_tag="VFI55_06320"
     CDS             3493..4062
                     /locus_tag="VFI55_06320"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7073682.1"
                     /translation="MAMNVCHRLCCNSDHWAKEVEEQLLPWALTDVDLGDNTLEIGPG
                     YGAFLRLLVDKTAQLTAVEIDTPMARRLQELYGDRARIINGDGTDTGLPADEFSSVVS
                     FTMLHHVPTAELQDRLFAEAFRVLRPGGVFAGSDSVTSLLFRVLHFRDTCTTVSPETL
                     PNRLRAAGFHDVDVEVREGRQRWRALKAA"
     gene            4062..4583
                     /locus_tag="VFI55_06325"
     CDS             4062..4583
                     /locus_tag="VFI55_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009478721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073683.1"
                     /translation="MHTEPDAAPTPSEQPQHSTRWARIRHQWSERLEPGEQATVLAWT
                     SFTLTFVGLRALTHWIRAGHGPSGGGISMGGKHFHHYNIGIGMLATVAGVGLRGTEAQ
                     RRHPAAAVAFGAANAMIVDELALLLDLKDVYWAADGRESVDVAVGVIATGATVVAGMP
                     FWPHVRHALRSAS"
     gene            complement(4654..5517)
                     /locus_tag="VFI55_06330"
     CDS             complement(4654..5517)
                     /locus_tag="VFI55_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HET7073684.1"
                     /translation="MGKEVPFVGTEAVDAGKFTERELRRSCMRIYRNVYQQRDSGLTA
                     KDRAVAAWLWSGKKAVVAGNSAAALLGAEWVDPHAPAELISGRKRPPRLIVTRNESLL
                     AGEVTVVNGIAVTTPARTAFDLGRRPGLVPAVIAIDSLARATGLAVEEVQPLVEAYRG
                     ARGVKQLREVLALVDAGAESPQETRTRLAIISAGLPAPRTQIVVHNQWGAVLARIDMG
                     WQQWCVGVEYDGAQHWTDPRIRAKDIDRIAELERRGWRIIRVSADLLRNRPDIVIARI
                     REALRAASCPC"
     gene            complement(5615..>7125)
                     /locus_tag="VFI55_06335"
     CDS             complement(5615..>7125)
                     /locus_tag="VFI55_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET7073685.1"
                     /translation="DVLDVWFDSGSMPYAQVHYPFENADWFDTHYPGDFIVEYIGQTR
                     GWFYTLHVLAIALFDRPAFKTCVAHGIVLGSDGQKMSKSLRNYPDVSEVFDRDGSDAM
                     RWFLMASPILRGGNLIVTEQGIREGVRQVLLPLWNAYSFLALYAPKVGNWRTDSTHVL
                     DRYILAKLAVLGDDLTQSMEVCDISGACEQLRQFTEALTNWYVRRSRTRFWEEDADAI
                     DTLHTVLEVTARLAAPLLPSVTEIIWRDLTAGRSVHLTDWPQVDELPVDPELVAAMDQ
                     VREVCSAGSSLRKAKKLRVRLPLPKLTVAVANPQRLAPFAELIADELNVKRVELTDAI
                     DTYGRFELTVNARVAGPRLGKDVQAAIKAVKAGEGVVNSDGTLTAGPAVLQPDEYSSR
                     LVAADPEYTAALPDGTGLVVLDGTVTPELEAEGWAKDRIRELQELRKSTGLDVSDRIR
                     VVMSVPAERADWAETHRVLIAGEILATSFQFGEPGQDAVGIGDGVQVAIAKA"
BASE COUNT         1193 a         2519 c         2301 g         1112 t
ORIGIN      
        1 atgccctgcc gctcgggcac gcctgatgtg gtgatgcggt agccggcagc ggcaaggccg
       61 ccgagcaccg tcgggccggt ccagcccacc gacgcgtgcg ccgccacggc agcgggcttg
      121 tcgaccgcga cgatgtcgtc gtcggagtaa aggatcgtca tgccttcgat gtcgacgggc
      181 gtgttctcca gcggtgccgg cgcgtcgggc agacgcacct gcagccaggc accgctcgtc
      241 aggcggtcgg actttcccgc cgcaacaccg tccagttcga cgccgccgtc ttcggccagc
      301 gccgccaccg cactgcggga cagccccagc aaacgcgcca gcccggcgtc cacacgcata
      361 cccgccagtc cctccgggac tggcatcgaa cggtcggtca acgctggtcg gccttacgcc
      421 gacccacggt gtccgcgtcg gcgttacgcc gaccaacggt gtccccgtgg gcgttacgcc
      481 gacccacggt gtcgaagtcg tagccgaaaa tcgacagcac cagcaggagg atggccccgc
      541 cgaccaccga ggggtcggcg acattgaaca ccggccacca gccgatcgag aaaaagtcca
      601 cgacgtggcc gcgcagcgga ccgggtgcgc ggaaaaagcg gtcgaccagg ttgcccatgg
      661 cgccgccgag gatcatccca agccccaccg cccaccaggg cgataccagc cgccgcccca
      721 tccagaagat gccgaccacc acacccgtcg cgatcagcgt cagtagccac gtgtatcccg
      781 tcgccatcga gaacgcagcg ccggaattac gtaccaaagt ccaggtcacc gtgtcaccga
      841 cgatcgacac cggctgaccg gggggcagca gtctgacggc cagcaccttg gtcacgatgt
      901 ccagggtgag caccaccgcc gcgaccgaca gcagcagacg cagtcgcctt ggcgggttgc
      961 gcgacccctg cggcgcggcg gccgcaggtt ttgcagcctc gtcggcgtca ccagccggtt
     1021 cggctgcggt caccggctcg gccggtcctg tcggttcgtc acgcaccctc ccatcatccc
     1081 ctaatgcgga tggtgcctcg ccgttgcccg ccggaggggt gcgcaatgat tcccccatgg
     1141 cccgcctcac cgtcatcgcc accggaggga cgatttcgac gagcaccggc cccgacgggg
     1201 tgcgccgacc ggcctacagc ggggcggagt tgatggcacc ccttggcgag ggcttggatg
     1261 tcgcagacgt cgacgtcgtc gacctgctgg cgctcgacag ctcacagctg acaccagccg
     1321 actgggatcg aattcgcgac gccgtgcagg ccacgatcga tgacggcgcc gacggtgtgg
     1381 tgatcgcgca cggcaccgac acgatggagg agagcgcctt atggctcgac ctcacccatg
     1441 ccggcgacgt cccggtcgtc ctcaccggcg ccatgcgaag cgctgatgca cccgacgccg
     1501 acggtccggc caatctgcgc gacgcactgg cggtggcggc cagcccggcc gcacgtggtc
     1561 tcggtgcgct ggtgtgcttc gcgggccggg tgctgcagcc gttgggcctg cagaaggtgg
     1621 ccacccagga cctgagcggc ttcgacggcg agctgatcgg caccgttgcc ggcggtgtga
     1681 cgctgacggg cgccaagaca cggccctacc tgggcgacct tcgcgccgcc gacgcaccgc
     1741 gggtcgacat tgtcgccgcg tacctgggca gtgactcagt cgcgctcgac gcctttgtga
     1801 tcgccggcgc acgggcggtg gtgctggaag cgctgggttc gggcaacgcc ggaaccgcag
     1861 tggtcgacgg ggtgcgccgc cactgccgca aagggatcgt ggtcgccgtg tccacccgag
     1921 tccccggcgg gcgggtcagc gcgggctacg gtcccggcca cgacatggcg gccgccggcg
     1981 cggtgatggt gccgcgactg cggccgccgc aggcgcgggt gctgatcatg gccgccctgg
     2041 ccgcgggcct acccatcgcc gaggtcatcg accgcttggg ctgaaagagc gttcgcctgc
     2101 agcacgccga cgtagtccgg ccagtccagg gagtcgacgg gattggcgcc ggcgaggtcg
     2161 gcggtatcgg cgggaaacgt gtgcgcctgg ccgggccccg agctacgggt ctcgaaacgc
     2221 accgtcacca cgccgtggcc cgcgccctgc acccagccgt gcccgagttc acggtgcgtg
     2281 acgtcgtctc ccacgcgcca cggcgcagcc tcggaaggcg gtgcgaacat ggcctcggtt
     2341 gcggttgctg cggtttgcac ggggtgttca tcggacgtct ccccgggcag ttccaagtcc
     2401 gggaacaacg actcctgacg cacctcgctc aaacccgaaa aaccaacgcc cagtaaacga
     2461 atcggcccga tctcgcgcgg gtcgagcagc agcctgcggg ccaccgcgat cagcgcgccc
     2521 gcctcggtcg tcgggtaggg cagcgtcgcc gagcgagtaa gcgtgctcat gtcggatttc
     2581 ttcagcttca ccgtgaccgt ccgggcgccg cgcccgtcgc gcagcaggcg ctgatgcgcg
     2641 tgctcggcga tcggctcgac cgcttcgcgc aattgctcca ggctggtcag atcgacggcg
     2701 aaggtcgatt cggcgctgat ctgcttggct tcggcacgct cggcgacggg acggtcgtcg
     2761 atgccacggg ccagccgatg cagcgccggc cctaccgtcg cgcccaggat gttggccgcc
     2821 tcggcgtcgg tcagcgcggc caggtccccg atcgtctcga tgccgagtcg gttcagcttc
     2881 tcccccgcga caggaccgat gccccacagc cgccgcaccg gcaatccgcc gagcagcgcg
     2941 tgttcttcgg cgcgccggac cacccggacg ccgtcgggct tggcaagggc ggacgcgatc
     3001 ttggcgatct gcttacccga gcccgcgccc acggacgcga cgagccccgt ctcctcgcga
     3061 acccgtcgcc gcaagccctc gcagaacacc gcgacgtcgt cggccgacgc cccggcgagt
     3121 tgggccggct caccgaaacc ctcgtcgaag gacagttgct cgacgacggg taccaggctg
     3181 cggacggtgt cgaacacccg acggctggcc accccgtaca ccacaccgcg cggcggcaac
     3241 accaccgcgg tcacgccgat cagccggcgc gcctgatgca tcggcatggc cgaccgcgca
     3301 ccgaacacgc gcgcctcata gctggccccg gccaccactc cccggccacc caacccgccg
     3361 accagcaccg gccgcccccg cagggtcggt cgggtgagtt gttcgacgga ggcgaaaaac
     3421 gcgtccatgt ccaggtgcag cacccaccgg gacttcacac ctgcaaatgg tattggtcta
     3481 gcgtcggggg gtatggccat gaacgtctgc cacagactgt gctgcaattc ggaccactgg
     3541 gcaaaagaag ttgaagaaca actgttgccg tgggcgctca ccgacgtgga cctgggcgac
     3601 aacaccctcg agatcgggcc gggatacggc gcatttctgc gactgctggt tgacaagacg
     3661 gcccaactca ccgcagtcga gatcgatacc ccgatggccc ggcggctgca ggagctgtac
     3721 ggcgaccgcg cgcgcatcat caacggcgac ggcaccgaca caggcttgcc ggcagatgag
     3781 ttcagctcgg tggtgtcgtt cacgatgctg caccacgtcc ccaccgcgga actgcaagac
     3841 cggctgtttg ccgaggcctt ccgggtgctg cgtcccggcg gcgtcttcgc gggcagcgac
     3901 agcgtgacat cactgctgtt ccgggtcctg catttccgcg acacctgcac caccgtctcg
     3961 ccggaaacct tgccgaatcg cttgcgcgca gcgggctttc acgacgtgga tgtcgaagtc
     4021 cgtgaaggca ggcagcgctg gcgcgcgctg aaggcggcgt aatgcacacc gagccagacg
     4081 ccgcgccgac accgtccgag caaccgcagc actccacgcg atgggcgcgg atacgccatc
     4141 agtggagcga gcgactggaa cctggcgaac aggccacggt cctggcctgg acgtcattca
     4201 ccctgacgtt cgtcgggctg cgcgcgctca cccattggat ccgtgccggc cacggaccgt
     4261 cgggcggcgg tatcagcatg ggcggcaagc actttcacca ctacaacatc ggcatcggga
     4321 tgctggcgac ggtggcgggc gtcggtctgc gcggaacaga agcgcaacgg cgacaccccg
     4381 cggcggcagt cgcgttcggt gcagccaacg caatgatcgt cgacgagctg gccctgctgc
     4441 tcgatctcaa ggacgtctac tgggcagcgg acgggcgcga aagcgtcgac gtcgcagtcg
     4501 gtgtcatcgc caccggcgcg acggtcgtgg caggcatgcc gttctggccg cacgtccgcc
     4561 acgcgctgcg gtccgctagt tgaacccgag ccgcgaaagt gcaactaccg accgcggctc
     4621 tcgggaaacg cgttgctagt tacactttcg cggtcagcag ggacagcttg cggctcgcag
     4681 tgcttcgcgg atccgagcta tgacgatgtc cggtcgattg cgcaacaggt cggcgctcac
     4741 ccgaatgatg cgccagcccc ttcgctccaa ttcggcgatg cggtcgatgt ctttggcgcg
     4801 gatgcgcggg tcggtccagt gttgcgcgcc gtcgtattca acaccgacgc accactgctg
     4861 ccaccccatg tctatgcgcg ccagcacggc tccccactgg ttgtggacga caatttgggt
     4921 tcgcggcgcg ggtaaacccg cggagatgat cgcgagcctg gtcctggtct cctgtggcga
     4981 ttctgcgccg gcatcaacca aggccagcac ttcccgcagc tgcttcacac cacgagcgcc
     5041 gcggtacgcc tcgaccagcg gttggacctc ttcgaccgca agcccggtag ccctcgccaa
     5101 cgaatcaata gcgatgacgg cgggcaccag tccgggacgc cgcccaaggt cgaacgcggt
     5161 gcgggcaggc gttgtgaccg cgatgccgtt gaccactgtg acctcgccgg ccaacagcga
     5221 ttcgttccgg gtgacgatca accgtggtgg acgtttgcga ccgctgatga gttccgccgg
     5281 tgcgtgagga tcgacccact ccgcgccgag tagcgcagcc gctgaatttc cggcgacgac
     5341 agccttcttt cccgaccaca gccaggcggc taccgccctg tctttcgccg tcagcccgct
     5401 gtcgcgttgt tgatagacat tgcgatagat ccgcatgcac gaccgtcgca gctcgcgttc
     5461 cgtgaacttg ccggcgtcga cagcttcggt gccgacgaat ggcacttcct tccccatggc
     5521 cgcagactgc catgggcttc tgacaagccg cgaaagtgcg actaccgaca cccacacctg
     5581 ggaaacgtgt cgctagttgc actttggcgg cgtgctaggc cttggcgatc gccacctgga
     5641 cgccgtcgcc gatgccgacg gcgtcctgtc ccggctcgcc aaactggaaa ctggtagcga
     5701 ggatctcccc cgcgatcaga acgcggtgcg tctccgccca gtcggcccgc tcggcgggca
     5761 ccgacatcac caccctgatc cgatctgaga cgtccaaccc agtcgacttg cgcaattcct
     5821 gcagctcgcg gatccggtcc ttggcccagc cctctgcctc cagctccggg gttaccgtgc
     5881 cgtccaacac gaccagcccg gtcccgtcgg gcagcgccgc ggtgtattcc gggtcggcgg
     5941 ccaccagtcg tgagctgtac tcgtcgggtt gcaataccgc aggccccgcg gtcagcgtcc
     6001 cgtccgagtt gacgacgcct tcgcctgcct tgacggcttt gatggccgcc tgcacatcct
     6061 tgcccaaccg cggcccggcc acccgcgcat tcacggtgag ctcgaaccgg ccgtaggtgt
     6121 cgatcgcgtc ggtcagttcg acgcgcttga cgttgagctc gtcggcgatc agctcggcga
     6181 atggcgccaa tcgctgcggg ttcgccaccg ccaccgtcag tttcggcagc ggcaggcgca
     6241 cccgcaactt tttggccttc cgtagcgagg acccggctga gcacacctcc cggacctgat
     6301 ccatcgcggc caccagctcg gggtcaacgg gcaactcgtc gacctgtggc caatcggtca
     6361 ggtggaccga acgccccgcg gtcagatccc gccagatgat ctcggtcacc gacggaagca
     6421 gtggcgcggc cagccgcgca gtcacctcca gcacggtgtg cagggtgtcg atggcgtcgg
     6481 cgtcttcctc ccagaacctg gtacgagacc gtcgcacata ccaattcgtc aacgcctcgg
     6541 tgaactgacg cagctgctcg caggccccgg agatatcgca cacctccatc gactgagtga
     6601 ggtcgtcacc cagcaccgcc agcttggcca ggatatagcg gtccagcacg tgggtcgaat
     6661 cagtgcgcca gttaccgact ttcggcgcat aaagcgccaa gaagctgtag gcgttccaca
     6721 acggcagcaa cacctggcgt acaccttcgc ggatgccctg ctcggtgacg atcaggttgc
     6781 caccacgcag gatcggcgag gccatcagga accaccgcat ggcatcggag ccgtcgcggt
     6841 cgaacacctc ggagacatcg gggtagttgc gcagcgactt gctcatcttc tggccgtcgg
     6901 agcccagcac aatcccgtgc gccacacagg ttttgaacgc cgggcgatcg aagagtgcga
     6961 tggccagcac atgcagcgtg tagaaccagc cacgggtctg cccgatgtat tcgacgatga
     7021 aatcccccgg atagtgcgtg tcgaaccagt ccgcgttctc aaacggatag tgcacctggg
     7081 catacggcat cgagcccgag tcgaaccaca cgtcgagcac gtcgg
//
LOCUS       DASSGQ010000137         4109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_800116,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000137 DASSGQ010000000
VERSION     DASSGQ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800116"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VFI55_06340"
     CDS             complement(<1..786)
                     /locus_tag="VFI55_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014989003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HET7073686.1"
                     /translation="MKRLSAIPFDTFLLAVAATVALAALVPAHGEAADAVSDAAKAAI
                     ALLFFLYGTRLSPEQAWHGVRQWRLHLLVLATTFVIFPILGLAARTLVPAVLTIDLYK
                     GLLFLCLVPSTVQSSIAFTSIARGHVSAAIVSASLSNILGVVLTPLLVVLLMKTSGVP
                     RVDGTAVRDIMVQLLLPFAAGQLARPWLAGTVARYAALLKVVDRGSILLVVYTAFSMG
                     VVKGIWASVDVWRLVLVTVVAAVLLALVLTTTTVIGRLARLDRG"
     gene            complement(814..1155)
                     /locus_tag="VFI55_06345"
     CDS             complement(814..1155)
                     /locus_tag="VFI55_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073687.1"
                     /translation="MLIAGVLCMFGAVASAGFGTWSLAHSRAVDDTQLALRAMAPTQL
                     AAAVMLAAGGVVTLAASPHAALVVLIVCVAGAVGTLAAGSLQSARFALREAPAAPSCA
                     GQCAICTQSCH"
     gene            complement(1221..2483)
                     /locus_tag="VFI55_06350"
     CDS             complement(1221..2483)
                     /locus_tag="VFI55_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqN/LpqT family lipoprotein"
                     /protein_id="HET7073688.1"
                     /translation="MIQIARTLRVVTGGLAAGAIGVTVFAGATASADPLLPAPSPTPA
                     VPAPAAAPQNITAAPGQAPTNRLLAAPPAANPFVPNSATAATPPGVPAPAAVAPMPPT
                     LTPTVSGTIREYLQAKGVKFEAQKPQGFKPLDITLPVPTRWTQVPDPNVPDAFAVIAD
                     RHGNSIYSSNAQVVVYKLVGNFDPKDAMEHGYVDSQKLLAWETTNASMADFGGFPSSI
                     IEGTYRDGDITLNTSRRNVIATSGHDKYLVSLAVTTDRAVAVGDAPATDAIINGFRVA
                     TPGAATLSPAQAPGTAPVGLPAQSPAAAPVGVPAQAPATAPVPAQAPAAAPVGVPAQA
                     PAMAPAGVPATAPVPAQAPVGVPAQPPTAAPVGVPAQAPVMGPGGLPAQTPVPSQLAA
                     AQPRPAPNLLALVPGLPPLPNFAFLQGR"
     gene            2669..>4109
                     /locus_tag="VFI55_06355"
     CDS             2669..>4109
                     /locus_tag="VFI55_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET7073689.1"
                     /translation="MTESPTAAPGSASASGAAAPDSDAPPHRYTAALAGRIESTWQGN
                     WAKLGTFNVPNPVGSLAPTDGSPVPDDKLFVQDMFPYPSGDGLHVGHPLGYIATDVYA
                     RYFRMNGRNVLHALGFDSFGLPAEQYAVQTGTHPRTRTEANVVNFRRQLGRLGLGHDS
                     RRSFSTTDVEFYKWTQWIFLQIYNAWFDTSANKARRIAELIAEFDSGARRLEDGRDWA
                     KLSVGERADVIDGHRLVYRADSMVNWCPGLGTVLANEEVTADGRSDRGNFPVFRKRLR
                     QWMMRITAYADRLLDDLDLLDWPDQVKTMQRNWIGRSTGAMALFSASKSSGETVDIEV
                     FTTRPDTLFGATYLVLAPEHDLVDELVVTAWPDGTDPRWTYGAAAPTDAVATYRRAIA
                     AKSDLERQESRDKTGVFLGSYATNPANGKPVPIFIADYVLAGYGTGAIMAVPGHDQRD
                     WDFAHAFHLPIVEVIAGGDISEAAYVGDGV"
BASE COUNT          700 a         1337 c         1453 g          619 t
ORIGIN      
        1 gccgcggtcg aggcgggcca gccggccgat gaccgtggtg gtggtcagca ccagtgccag
       61 cagcacggcg gcgacgacgg tcaccaagac cagccgccac acgtcgacgc tggcccatat
      121 tcctttgacg acgcccatcg agaaagccgt gtacaccacc agcagaatcg atccgcggtc
      181 gaccaccttc agcagtgccg cgtagcgggc gaccgtcccg gcgagccatg gccgcgccag
      241 ctgccccgcc gcgaacggca gcaacagctg caccatgatg tcgcggaccg cggtgccgtc
      301 cacccggggt acgccgctgg tcttcatcag taacaccacc agcagcgggg tcagcaccac
      361 accgaggatg ttggacaacg acgcgctgac gatggcggcc gagacgtggc cccgtgcgat
      421 agacgtgaac gcgatggacg actgcaccgt cgaggggaca aggcagagaa acagcagacc
      481 tttgtacagg tcgatggtca gcaccgcggg aaccagcgtt cgagcggcca gccccagaat
      541 cgggaaaatc acaaacgtcg tggccagcac caacagatgc aggcgccatt gacgcacacc
      601 gtgccaggcc tgctcgggcg acaaccgggt gccgtagagg aagaacagca gcgcgatcgc
      661 cgcttttgct gcgtcggaca cggcgtcggc cgcctcgccg tgggcgggga cgagcgcggc
      721 aagggccacc gtcgccgcca ccgcgaggag gaaggtatcg aaagggatcg ccgaaagccg
      781 cttcaccgcg acggcttcag cacaaggcag gggtcagtgg caggactggg tgcagatggc
      841 gcactgaccg gcgcagctcg gcgctgccgg tgcctcgcgt agcgcgaaac gcgcgctctg
      901 caacgatccg gcggccaggg tgccgacggc gccggcgacg cagacgatca gcacgaccaa
      961 cgcggcgtgc ggcgaagcgg ccagcgtcac tacgccgccg gcggcgagca tcaccgcggc
     1021 cgccaattgg gtgggcgcca tggcgcgcag cgccagctga gtgtcgtcaa ccgcacggct
     1081 gtgggctagc gaccaggtcc cgaacccggc ggaggccacc gccccaaaca tgcacagcac
     1141 gcccgcgatc aacatgggct cacaatacga ggccgaccgc gattccgctc gcccggctca
     1201 gggctcttgt cggccccggt tcagcgccct tgcaggaagg cgaagttcgg cagcggcggc
     1261 agcccgggca ccagggccaa tagattcggt gccggccggg gctgggcggc ggccaactgg
     1321 ctgggaaccg gtgtctgagc agggagcccg cccggcccca taacgggtgc ttgggcgggg
     1381 actccgacgg gcgccgcagt gggtggctgg gcgggaactc cgacgggtgc ctgcgcggga
     1441 accggcgccg tggcggggac cccggcgggc gccatagcgg gtgcttgggc ggggactccg
     1501 acgggcgctg cagcgggtgc ctgcgcggga accggcgccg tagcgggtgc ttgggcgggg
     1561 actcctacgg gtgccgcagc gggtgactgg gcgggaagcc cgaccggagc cgtgccaggc
     1621 gcttgggcgg gcgagagggt tgcagccccg ggcgtggcca cccggaaccc gttgatgatc
     1681 gcgtcggtgg ccggtgcgtc tccaaccgcc accgcccggt cggtggtgac cgctagcgac
     1741 accaggtatt tgtcgtgtcc ggaggtggcg atgacgttgc ggcgcgaggt gttcagcgtg
     1801 atgtcgccgt cgcggtaggt gccctcgatg atcgacgacg ggaagccgcc gaagtcagcc
     1861 atggacgcgt tggtggtctc ccaggcgagc agcttctggc tgtctacgta gccgtgctcc
     1921 atggcgtctt tgggatcgaa gtttccgacc agcttgtaga ccaccacctg cgcatttgac
     1981 gagtaaatgc tgttgccgtg ccggtcggcg atcaccgcga aggcatcggg cacgttgggg
     2041 tcgggcacct gagtccagcg ggtgggcacc ggcagcgtga tgtcgagtgg cttgaatccc
     2101 tggggcttct gcgcctcgaa cttgacaccc ttcgcctgta ggtactcacg gatggtgccg
     2161 gagaccgtgg gagtcagcgt cggaggcatc ggtgcgaccg cggcaggtgc gggcactccc
     2221 ggcggtgtcg ccgcagtcgc ggagttcggc acgaacggat tcgccgccgg cggggcggcg
     2281 agcaacctgt tggtgggggc ctgcccaggg gcggccgtga tgttctgcgg cgccgccgcc
     2341 ggggcgggga cggccggggt gggggagggg gccggaagca agggatcagc cgatgcggtg
     2401 gcacccgcga agaccgtgac accgatcgcc ccggcggcca gacctcctgt gactacccgc
     2461 aacgtacggg cgatctgaat catctccgtc ggtccttccc catggcacgg gtaacagcac
     2521 cctgcgttcg aggctgactg taaccagtgg tcaaacgcgg gaacaggctc gaaatagagc
     2581 tgggatgaag ctctgatcgg cgtgcaacgg aaccgagacc aacctgtggc cggctggccc
     2641 ggcgtggcgg cccttatacc ctgttcaggt gaccgaatcc ccgaccgccg cccccggcag
     2701 cgcctccgcc tccggcgccg ccgcgccgga ctccgatgcg ccgccgcatc gttacaccgc
     2761 ggcattggcc ggccgtatcg aaagcacctg gcagggcaac tgggcgaagc tcgggacgtt
     2821 caacgtgccc aacccggtcg gctcgctcgc gccgacagac ggcagcccgg tgcccgacga
     2881 caagttgttc gtgcaggaca tgttccccta tccatcgggt gatggactgc acgtgggcca
     2941 cccgttgggc tacatcgcca ccgacgttta cgcccgctat ttccgcatga atggccgtaa
     3001 tgtgctgcac gcgttgggtt ttgactcctt cgggttgccc gccgagcagt acgctgtgca
     3061 aaccggcacc catccgcgca ccaggaccga ggccaatgtc gtgaacttcc ggcgccagct
     3121 gggccggttg ggtctcggcc atgacagccg gcgcagcttt tccaccaccg atgtcgagtt
     3181 ctacaagtgg acccagtgga tcttcctgca gatctacaac gcctggttcg acacctcggc
     3241 gaacaaggct cgccgaatcg ccgagctgat cgccgaattc gactccggtg cgcgccgtct
     3301 cgaagatggc cgggactggg cgaaactgtc ggtgggggag cgggccgacg tgatcgacgg
     3361 ccaccgcctg gtgtatcggg ccgactcgat ggtgaactgg tgtccggggc tgggcacagt
     3421 gctggccaac gaggaggtca ccgccgacgg gcgcagcgac cgcggtaact tcccggtgtt
     3481 ccggaaacgg ttgcggcagt ggatgatgcg tatcaccgcc tatgccgacc ggctgctgga
     3541 cgatctcgat ttgctggatt ggccggatca ggtgaaaacc atgcagcgca actggatcgg
     3601 ccggtccacg ggagccatgg cgctgttctc ggcgagtaag tccagtggcg aaaccgtcga
     3661 tatcgaggtg ttcaccacca ggcccgacac cttgttcggc gccacgtacc tggtgctggc
     3721 gcccgaacac gacctggtcg acgagctggt cgtcaccgcg tggccggacg ggaccgaccc
     3781 gcgttggacc tacggcgctg ccgcgccgac cgacgcggtc gccacttacc gacgtgcgat
     3841 cgccgccaag tcagatcttg agcgtcagga gagcagggac aagaccggcg tcttcttggg
     3901 cagttatgcg acaaatccgg ccaacggcaa gccggtgccc atcttcatcg ccgactatgt
     3961 gttggccggc tatggcaccg gcgcgatcat ggcggtcccg ggccacgacc agcgggactg
     4021 ggacttcgcc catgcttttc atctgccgat cgtcgaagtc attgctggcg gcgacatttc
     4081 ggaggccgca tacgtcggcg acggcgtgc
//
LOCUS       DASSGQ010000138         4950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_496589,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000138 DASSGQ010000000
VERSION     DASSGQ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496589"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFI55_06360"
     CDS             <1..372
                     /locus_tag="VFI55_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019667966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET7073690.1"
                     /translation="VGGQFDGAPLVVLHTVGARSGKPRTNIMMYLADGDRYLVFASKA
                     GAADNPAWYWNLKANPDARIEVGDDVVDVHATELRGAERDEKYALQAQRYPGFAEYER
                     KTSRTIPVVALTPTSPAEPRA"
     gene            700..1581
                     /locus_tag="VFI55_06365"
     CDS             700..1581
                     /locus_tag="VFI55_06365"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HET7073691.1"
                     /translation="MRKLMAAAAAVLAVGTCGANLGMATALADNMQPIGSVPIPDGPA
                     QTWIVADLDSGQVLAGRDQDVVHPPASTIKVLLALVALDEVSLDSTVVADAADTEAEC
                     SCVGIKPGRTYTARQLLDAILLVSGNDAANTLAHMLGGQDATVAKMNAKAAALGATST
                     HAATPSGLDGPGGSGGSTAHDLVVIFRAALANPVFAQITAEPSAMFPGDNGEQPITNR
                     DELLERYPGAIGGKTGFTNAARKTFVGAAARGGRRLVIAMMYGLVREGGPTYWDQAAS
                     LFDWGFALNPESGVGSL"
     gene            complement(1612..2205)
                     /locus_tag="VFI55_06370"
     CDS             complement(1612..2205)
                     /locus_tag="VFI55_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7073692.1"
                     /translation="MARTQQQRREETVGRLLEACIATIVEVGYARASAAVITKRAGVS
                     VGALFRHFETMGDFMAATASEVLRRQLESFTKQVAEIPADRPALESALAILRDITGGP
                     TNAVLYELLIAARTDEKLRDTLQHELGQYSAKIYDAARAMPGAEAFPEETFPVIVALL
                     TNVFDGAAVVEGVLPQPHIAEQRIPVLTALITGALPG"
     gene            complement(2207..4028)
                     /locus_tag="VFI55_06375"
                     /pseudo
     CDS             complement(2207..4028)
                     /locus_tag="VFI55_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878717.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            complement(4118..>4950)
                     /locus_tag="VFI55_06380"
     CDS             complement(4118..>4950)
                     /locus_tag="VFI55_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003914920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HET7073693.1"
                     /translation="HAAAEPTVPAVARRGNDISATRAAVTRVPVVTAPTRRRPRKAVL
                     AAAALVLLGGLLAVGLVAMRKTTVTATPTTASTEAAASPTAGAPAVPVAPTSNAPFVA
                     PAPLDGTYRVEVQRAKQTFNYTPDPQTPDMDTWLDVRTSCTPTSCTAVGVPLDDGDHT
                     QAKSAGAAPVVFDFTDGRWLSRPENTTFPCVGSNGTQGTHATTQVLSLRPQPPGGLVG
                     EMTVTVQSNECDQLGAVVRAPAVADRTGDPPPDVAVPDPATLPGDTAVPTSELPGPPR
                     "
BASE COUNT          786 a         1717 c         1714 g          733 t
ORIGIN      
        1 gtcggcggcc aattcgacgg tgcaccgctg gtcgtcctgc acaccgtcgg tgcacgcagc
       61 ggtaagccgc gcaccaacat catgatgtat ctggccgacg gcgaccggta cctggtgttc
      121 gcctcgaagg ccggcgccgc cgacaacccc gcctggtact ggaacctcaa ggccaacccc
      181 gacgcgcgca tcgaagtcgg cgacgacgtc gtcgacgtgc atgccaccga gctgcgggga
      241 gccgaacggg acgagaagta cgcgctgcag gcccagcggt atccgggatt cgccgagtac
      301 gagcgcaaga cctcgcggac catccccgtg gtggcgctca cgcccaccag tcccgcggag
      361 ccgcgcgcat gaaggtgctg gcaaccggcg cgacggggca gtacgcccat cacgtcgtcg
      421 ccgccctcgt cgcccaagga atcaacgtac gcggcgtcgt tcacgatccg gcgaaggtcg
      481 acgaggctcg cacggcccgt gcaaccgaga ccgtgcaggt ggatctggcc gacgccgacg
      541 gggtcaccga ggcacggatt tcacggggga aatgcgctgg tcctgcgagc catcctgcgc
      601 cgcgaaccgc gctcagtcgc cgacttcatc gccgagctcg cgcggtaagg cccggctgca
      661 acaggcgacg cggacgcccg gaagttaaac tttgcgccca tgcggaagct catggccgct
      721 gccgccgccg tgctggcggt cggtacctgc ggcgccaacc tcggcatggc cactgcgctg
      781 gccgacaaca tgcaaccgat cggctcggtg ccgatccccg acgggccggc ccaaacctgg
      841 atcgtggccg acctcgacag cggtcaggtg ctggccggcc gggaccagga cgtcgtgcac
      901 ccgcccgcga gcaccatcaa ggtgctgttg gcgctggtgg cgctggacga ggtcagcctg
      961 gactcgaccg tcgtcgccga cgccgccgac accgaggccg aatgcagctg cgtcggcatc
     1021 aagccgggcc gcacctacac cgcgcgccag ctgctcgacg ccatcctgct ggtgtcgggc
     1081 aacgacgccg ccaacacgct ggcgcacatg ctgggtggcc aggacgccac ggtcgccaag
     1141 atgaacgcca aagccgcggc actgggggcg accagtaccc acgcggcgac cccgtcgggc
     1201 ctggacgggc ccggcggatc ggggggttcg accgcgcacg acctggtcgt catcttccgg
     1261 gccgcgctgg ccaatccggt gttcgcgcag atcaccgcgg agccgtcggc aatgtttccc
     1321 ggcgataacg gtgagcagcc gataaccaac cgcgacgaac tgctggagcg ctatcccggc
     1381 gccatcggcg gcaagaccgg gttcaccaac gcggcccgca agaccttcgt cggtgccgcc
     1441 gcccgcggcg gccgccggct ggtgatcgcg atgatgtacg ggctggtcag ggagggcggc
     1501 ccgacatact gggaccaggc cgcgagcctg ttcgactggg gtttcgccct caacccggag
     1561 tcgggagtcg gttcgctcta accgcgtgtc acaccgcgcc tcataccgcg gtcagccggg
     1621 caatgctccg gttatcaggg ccgtgagcac cggaatccgt tgctccgcga tgtgcggctg
     1681 cggcagcacc ccctccacca cggcggcccc gtcgaacacg ttcgtcagca gcgccacgat
     1741 gaccgggaac gtctcttccg ggaaggcctc ggccccgggc atcgcccgtg cggcgtcgta
     1801 aatcttggcc gagtactgac cgagctcgtg ttgcaaagtg tccctgagct tctcgtcggt
     1861 gcgagccgcg atcaacagct cgtagagcac cgcgttggtc gggccgccgg tgatgtcccg
     1921 caggatcgcc agcgccgatt ccagcgccgg ccggtcggcc ggtatctcgg caacttgctt
     1981 ggtgaacgac tccagctggc ggcgcagcac ctccgacgcg gtcgccgcca tgaaatcgcc
     2041 catcgtctcg aagtgacgga acagggcgcc caccgatacc cccgcgcgtt tggtgatcac
     2101 ggcggcggac gcgcgggcgt acccgacctc gacgatcgtg gcgatgcagg cctcgaggag
     2161 ccgaccgacc gtctcctcgc gacgctgctg ctgggtgcgg gccatgtcag gccccgactt
     2221 tttgggcgtt tcggccccgc cgcgaccctg cccgcaggta gcggcccgac ttcacggtgt
     2281 ggccgtagcc gtcccggaat cggccgttac ggaagacgac ggcgccgccc acgccggtcg
     2341 cgaccacggc gtcgtcgttg cggttgacca tgcggcgcag gccgccgtag aacggcacct
     2401 cttcctcgta gtagccgtcc accgattcgt tgaggccggc cgggtcgatc accacgaagt
     2461 cggcgcgatc accttcgcgc agggtgccgg cgtcgatgcc gaaccactcc gcgagttcgc
     2521 cggtgaggcg atgcacggcg cgctcggtgg acaaaaacgg agagcccgac cggtgcgcgt
     2581 cccgggtgcg cttgagcatc cgcaacggaa agttgtaaaa ggccatgttg cgcaggtgcg
     2641 ctccggcgtc cgaaaagccc atgtgcacgc tcggttcggc ggcgagcttg ttgagctgct
     2701 tcggccggtg gttggccacg atggtcgtcc accggacgtt gcgctcaccg ttttcgacga
     2761 gcacatcgag gaacgcgtcc agcgggtgca ggccgcgctc gtcggcgatc gcgccgaagc
     2821 tcttgccgat caacgactcg tcggggcact cgacgatcac ggcgtcgtgg aagtcgcggt
     2881 gccacaacga cggtccgagt ttgatgcggt cgaactcgcg ccggaaccgc cggcgatact
     2941 ccctgtcggc cagcagctca ttgcgctgca actgttcgcg cagatgcagg gccgccgttc
     3001 cggcgccgaa ctcttcgaag accggcaggt cgatgccgtc ggagtacagc tcgaacggca
     3061 ccggcaggtg ctggaaccga acgctggagc ccagaatcgc gttgagcagg cgggtgccca
     3121 gcccgaagac gtggacggcc agcggcatcg acttggcgtc ggccgacacc agcaggctca
     3181 tccgaactcc ccggcggcgg ccgaacatcc ggctgctcga caagaagaac agcagccccg
     3241 acatcgggtt gtcgacgtcg ggcgcgctct gcaggatccg gccccgtttg cgcagcacct
     3301 tgatcagcct gcgccgttcc cgccatgtcg cgaaggtgga cggcagcgcg cgcgaccgga
     3361 accggtcgcc gtcgagcttg tcgatggccg cgtccatccc ggacatgccg agcaccccgg
     3421 cctcgagcgc ctggtccagc aatgccgcca ttgtgttcag ttcggcagcg gtgggcctga
     3481 cggtcgcgtc ggtggcgcgg tcaagtccca ggaccgcggt acgcaggtcc gaatgaccaa
     3541 gcagcgaacc gacattcggc cccagcggca gcgcgtcgat ggcctcgatg tactccgcgg
     3601 ccgtcgacca cgtcttgctg gcgttcagcg cgccgtacac gtactcgcgg ggcaccgcct
     3661 ccacccggct gaacaggtcg gcggcgtcct gggagctggc gtagaccgtc gacaacgagc
     3721 agttgcccaa cagcacagtg gtgacgccgt gccgcaccga ctcacgcagc ccggggtcca
     3781 gcagcacctc ggcgtcgtag tgggtgtgca cgtcgatgaa gccgggcacg atccacttgc
     3841 cggcggcgtc gatcacctcg ggggcaaccg gcgtcgtcca aaggcgcgtc ggagggcccg
     3901 gccaccgcgg ccaccacgcc gtcgcgaatg ccgagcgtgc gggtttgcgg cgcgctgccc
     3961 gtgccgtcga accagagccc atcgcggatg atcacgtcgt aggccactgt ttcctcctca
     4021 agccggataa cggcaaccgt accattagat agcaagcact cgcaatcttt cttacgacga
     4081 cggcatcacc caaaaccact ggtgggttac cgccggatca gcgaggcggc ccgggcagtt
     4141 cgctggtcgg caccgcggtg tcgccgggga gcgttgccgg gtcgggaacc gcgacatccg
     4201 gcggcgggtc gccagtgcgg tcggccaccg cgggggcccg gacgacggcc cccagctggt
     4261 cgcactcgtt gctttgcacg gtcaccgtca tctcacccac gagcccaccc ggcggctgcg
     4321 gccgcagcga cagcacctgc gtggtggcgt gcgtcccttg tgttccgttg gacccgacac
     4381 aagggaacgt cgtattttcc ggccgcgaca gccaacgacc gtcggtgaag tcgaagacga
     4441 cgggtgcggc accggccgac ttcgcctggg tgtgatcgcc gtcgtccagg ggcacgccga
     4501 cggcggtgca tgaggtcggc gtgcaggacg tgcggacgtc caaccaggtg tccatgtcgg
     4561 gcgtctgagg gtcaggcgtg tagttgaacg tctgcttcgc gcgttgcacc tcgacgcggt
     4621 acgtgccgtc gagcggggcg ggcgcgacga acggggcgtt ggacgtgggg gcgacgggaa
     4681 cggccggcgc accggctgtc ggactggcgg ccgcctcggt gctcgccgtg gtcggggtgg
     4741 cggtcacggt ggtcttgcgc atcgcgacaa ggccgacggc gagcagccca ccgagcagga
     4801 ccagtgccgc cgcggccagc acggccttgc gcggccggcg cctcgtcggt gcggtcacca
     4861 cgggcacccg ggtgaccgct gcgcgagttg cggaaatgtc attgccgcga cgggccacgg
     4921 cgggaacggt gggttcggcg gcggcgtgct
//
LOCUS       DASSGQ010000139         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_708930,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000139 DASSGQ010000000
VERSION     DASSGQ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708930"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..799
                     /locus_tag="VFI55_06385"
                     /pseudo
     CDS             3..799
                     /locus_tag="VFI55_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603444.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            912..1943
                     /locus_tag="VFI55_06390"
     CDS             912..1943
                     /locus_tag="VFI55_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7073694.1"
                     /translation="MTMPAVVFTADGRIALQERPRPPLRSDQVLVEIDLCGICGSDLH
                     ASQLPQVYLGDCILGHESTGRIAAVGDDVANWSVGQRVAVNPNGNVDGTCVYCRSGRP
                     NFCRQATLETALGLQADGALAPLMSAFPGHLRRIPDELGALEAAWVEPTATALRAVEL
                     AGELAARTVLVTGGGPIGQLACRLAHLRSPAQILLMEPAAQRAEFATESNAVLIAADA
                     AGDHRGGIAVDVVFECSGSAAATSTALKMLAPGGILVVVGAGPEPGLDSATILLKEIT
                     VRGSYIYTDEFDRAIQLLATRQIAVADLTTVISPLTDALTAFDGLRAGRIMKALIAPG
                     TVVSNSRLA"
BASE COUNT          348 a          668 c          656 g          339 t
ORIGIN      
        1 agctggccgg cgacggcgta ttcggcccac ccagggaccg tgacgaagcc atcgcggtgc
       61 tacgggccgc ggtggacgcc ggggtggagc acatcgacac cgcgcagtat tacgggcccg
      121 gggtggtcaa cgaaatcatc cgggaggcgc tttaccccta ccccgacggt ttggccttgg
      181 tgagcaaagt ggcggcccgc cgcgacgatc tcggggcggt gctaccgtac gacgaccccg
      241 ccagctgcgg tgcggcatac aggacaacct cacgacactg ggggtcgagc aacttgccgc
      301 cgtcaacttg cgggtgatgg acaacgcggt gccggacgct cggttcgtcg atcagctcgg
      361 tgcgctggtc tcagcccgcg acgagggcct gaccgccgca attgggatca gcaacgtgag
      421 ccgcgagcac ctgacgcggg cgttcgaggt gaccgaaatc gcctgcgtac agaacttctt
      481 caaccttgcg gctcggcaca gccagcctgt cttggaggac tgccgtgcgt gggagatcgc
      541 atttgtgcct ttctgtccct tgggttttcc tcgatcgcag cgcgaatcga tactaaccaa
      601 cccggccgtg gccggcgttg ccgctcagca cgaggcgacg gccgcgcagg tggctctcgc
      661 gtggttactg gccgtcgcac cgaacgtcct gctgatcccc ggcacgcgca cgcgcggcca
      721 cctggccgaa aaccttgccg cgggcgatct gcagctgagc gatcgcgaga tcgtgctcct
      781 caaccggacg ttcagctgac atgtgtccgc gacgcacggt attgcgggtg ctggctgcca
      841 tggtcgcctg gggccctttc gggccgcatc cgcatactta gtaacgaact tcgacaatcg
      901 aggcgctgaa gatgacaatg cccgcggtgg tgttcaccgc tgacggacgg atcgcactac
      961 aggagcggcc acgcccgcca ctgcgctccg accaagtcct ggtcgaaatc gacctatgcg
     1021 gcatttgcgg cagcgatctg cacgcatcgc agctgccgca ggtgtacctc ggtgactgca
     1081 tcctcggaca cgaatccact ggccggatcg cggcagtcgg cgacgacgtc gcgaactggt
     1141 cggtcggcca gagggtcgcc gtcaacccca acggcaacgt ggacggcacg tgcgtgtact
     1201 gccggtcggg acggccgaac ttctgtcggc aagccacttt ggagacggcg ctgggcctgc
     1261 aggccgatgg ggcgctggcc ccgttgatga gcgcattccc cggacacctg cgccgaattc
     1321 ccgacgagct gggggcgctc gaagccgcct gggtagagcc cacggccacc gcgttgcgag
     1381 cggtggaact cgcgggcgag ctggccgcac ggacggtatt ggtaaccggc ggcggcccaa
     1441 tcgggcagct cgcctgcagg ctcgcacacc tcagatcgcc cgcccaaatc cttctcatgg
     1501 agccggccgc gcagcgtgcc gaattcgcga ctgaatccaa tgcggtgttg atcgccgccg
     1561 atgcagccgg agaccaccgt ggcggaattg cggtcgacgt cgtcttcgaa tgctcaggca
     1621 gcgccgccgc cacctcgacc gcgctgaaaa tgcttgcccc gggcggcatc ctggtggtgg
     1681 tcggggccgg acccgaaccc ggccttgact cggcgaccat tctgctcaaa gagattacgg
     1741 tgcggggctc gtacatctat accgacgaat tcgaccgcgc gatccagctg ttggccaccc
     1801 ggcaaatcgc ggtcgcagac ctcaccacag tcatcagtcc actgactgat gcgctgaccg
     1861 cattcgacgg gcttcgcgca ggccgaatca tgaaagcgct catcgcgccg ggcaccgttg
     1921 tgagcaactc acgccttgcc tagtccagct gaaaccttgt ggcgccgaac ggttccgacg
     1981 tgcccagtgc gaacacggtc gaaggtttca g
//
LOCUS       DASSGQ010000140         4667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_860817,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000140 DASSGQ010000000
VERSION     DASSGQ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860817"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFI55_06395"
     CDS             <1..325
                     /locus_tag="VFI55_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HET7073695.1"
                     /translation="PVTVSDLLAARPDPFCEIEASLLWHLDSAHQDVVARLVSKLPAP
                     LRRGQVRPLGLDRYGVRFRVERGDGDHDVRLPFHTPVQDMCGLSQAIRVLMGCPFING
                     LRARR"
     gene            381..1631
                     /locus_tag="VFI55_06400"
     CDS             381..1631
                     /locus_tag="VFI55_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET7073696.1"
                     /translation="MNGERSPERHGPREPRPPIPPAQARPEASQVIHRAPGPAPSPND
                     QTMPLHRPSPPAAPINPPVPPPRPVTPAPRARPSAQSGPPPRPVAPRARRKRGWRRRA
                     RAVLLVLLIGSLLTGAGVIGATFALDSALHREQALTDYPDRPAPGRGTNWLLVGSDSR
                     QGLTAEQQQDLATGGDTGNGRTDTVLLVHVPEFSSGTPTTMVSIPRDSYVAIPDHGKD
                     KINAAFATAGAPLLTHTVELATGLRLDHYAEIGFSGFAGLVDALGGVTLCPAAPINDP
                     LAGIDLPAGCQQLDGRNALGYVRTRDTPRADLDRMVNQRQFLSALLERAASPAVWLDP
                     SRWYSVPRAAVDALTVDRGDHAWDLARLGWALHGSPTTMTVPIGEFITNDAGSVVVWD
                     HEAAGKLFDALRSDEPVPALDGQQ"
     gene            complement(1652..2476)
                     /locus_tag="VFI55_06405"
     CDS             complement(1652..2476)
                     /locus_tag="VFI55_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET7073697.1"
                     /translation="MTWAEEVLAGHPFVVAHRGASASRPEHTLAAYDLALKEGADGVE
                     CDVRLTRDGHLVCVHDRRLDRTSTGAGLVSTMTLAELRELEYGAWHDSWREDGTHGDT
                     SLLTLDALVSLVLDWHRPVKIFIETKHPVRYGSLVENKLLASLHRFGIAAPASADRSR
                     AVVMSFSAAAVWRIRRAAPLLPTVLLGRTGRYLTSSAATAVGATAVGPSLAALKDYPQ
                     LVDRAVAQGRAVYCWNVDEYEDIDFCRDVGVAWLATHHPGRTKAWLENRSAGQGGG"
     gene            complement(2481..3527)
                     /locus_tag="VFI55_06410"
     CDS             complement(2481..3527)
                     /locus_tag="VFI55_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HET7073698.1"
                     /translation="MIQVCSQCGTRWNVRERRREWCPRCRGALMAPLADVPAADPRWS
                     AQGRPPSARPPAARGWQRTPPPLPPGYRWIAVRPGAAPPSRHGRRPLGPTPRYAVIPR
                     WGLADLVEQAPAAAEKPTQAGPSAAVVRTALFLSVLVLAFAALVYVVRYVLLVINRDT
                     LLNSIVAIAADWLGILASVAAIAAVITSGVMLIQWLVARRAAVFAHLGLPEPRSVRAL
                     WAGCVVPLANLLWAPVYVIELATIEERYARLRTPIVRWWFAWAFSYLVSIFAIATSFA
                     TDAQGIANNTVLMVFAYLFAGVTMAAVARVFEGFESKPVERPAHRWVVVTDDQPVTVS
                     SAGPVELEGQEPAA"
     gene            4339..>4667
                     /locus_tag="VFI55_06415"
     CDS             4339..>4667
                     /locus_tag="VFI55_06415"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET7073699.1"
                     /translation="MAEYTLPDLDWDYAALEPHISGQINEIHHSKHHAAYVKGVNDAV
                     AKLEEARAKDDHSAIFLNEKNLAFHLGGHVNHSIWWKNLSPNGGDKPTGELAAAIDDA
                     FGSFDKFR"
BASE COUNT          789 a         1666 c         1492 g          720 t
ORIGIN      
        1 gcccgtcacc gtctccgacc tgctcgcagc ccggcccgac ccgttctgcg agatcgaggc
       61 aagcctgctg tggcacctgg acagcgccca tcaagacgtg gtggcgcgac tggtgtcgaa
      121 gctgcccgcg ccgctgcgac gcgggcaggt ccgcccgctt ggactcgacc gatacggcgt
      181 gcggtttcgc gtcgaacgcg gcgacggcga ccatgacgtt cggctgccct tccacactcc
      241 ggtgcaggat atgtgcgggc tgagccaggc gatccgggtg ctgatgggtt gcccgttcat
      301 caacgggctg cgcgcccgca ggtaatccct cgctctcgcg cctgccgcac gatcgcggcg
      361 gcggcacgta aattatctgg gtgaacggcg agcgatcacc ggaacggcac gggcctcgag
      421 agccccggcc accgatcccg ccggcgcaag cacggccaga ggcgtcgcag gtcattcacc
      481 gcgcgccggg cccggcgcca tcgccgaacg atcagaccat gccgctgcac cggccgtcgc
      541 cgccggccgc ccccatcaat ccgcccgtcc ccccgccgcg accggttaca cctgcgccgc
      601 gggccagacc gtcggcgcag tcggggcctc cgccgcgacc cgtcgccccg cgggcgcgcc
      661 gcaaacgcgg ctggcgccgc cgggcccgtg cggtattgct cgttctgctg atcggctcgc
      721 ttctcaccgg cgccggcgtc atcggcgcga cgttcgcgct cgactccgcg ctgcatcgcg
      781 agcaggcact cacggactat cccgaccggc cggcgcccgg ccgtggcacg aactggctgc
      841 tcgtcggctc ggacagccgc cagggactca ccgccgaaca gcagcaagac ctggccaccg
      901 gcggcgacac cggcaacggc cgtaccgaca ccgtcctgct ggtgcacgtg ccggagttct
      961 cgtccggcac cccgaccacg atggtgtcga tacctcgcga ctcctacgtg gcgattccgg
     1021 atcacggcaa ggacaaaatc aacgccgcgt tcgcgacggc cggagccccc ctgctcaccc
     1081 acacggtgga gctggccacc gggttgcgcc tcgaccacta cgccgagatc ggattcagcg
     1141 gtttcgccgg cctggtcgac gccctcggcg gggtcacgtt gtgccccgcc gcgcctatca
     1201 acgatccgtt ggccggcatc gacctgccgg ccggctgtca gcagttggac ggccgtaacg
     1261 cgctcggata cgtcaggacg cgcgatactc cgcgcgcgga cctggaccgc atggtcaatc
     1321 aacggcagtt cctgtcggcg ctgttggagc gtgccgcgag cccggcggtg tggctcgacc
     1381 cgtcccgctg gtactcggtg ccgcgtgccg ctgtcgacgc cctgaccgtc gaccggggcg
     1441 atcacgcgtg ggatctggcc cgactcggct gggcgctgca tggatcgccc accacgatga
     1501 ccgtcccgat cggggagttc atcaccaacg acgccgggtc ggtggtggtg tgggatcacg
     1561 aagccgcggg caagttgttc gacgcgttgc gctccgacga gccggtgccc gctctcgacg
     1621 gacagcaata gcgtccatct tcgcgcgcca gctagccgcc gccctgaccg gcgctccggt
     1681 tctccaacca ggccttcgtg cggcccgggt ggtgggtggc gagccaggca accccgacgt
     1741 cccgacagaa gtcgatgtct tcgtattcgt cgacattcca gcagtacacc gccctgcctt
     1801 gcgcgacggc gcgatccacg agttgtgggt aatccttcaa cgccgccagc gagggcccga
     1861 cggcggtggc gccgacggcg gtggccgcgc tgctggtcag gtagcggccg gttctgccga
     1921 gcagcaccgt cggcaacagg ggcgccgctc ggcggatccg ccagaccgcg gccgccgaga
     1981 acgacatcac caccgcgcgg gaccggtccg cggaggcagg cgcggcgatg ccgaaacggt
     2041 gcaacgaggc gagcagcttg ttttccacca gtgagccgta acggaccggg tgcttggttt
     2101 cgatgaaaat cttcaccgga cggtgccagt ccaggaccag cgaaacgagg gcgtcgagcg
     2161 tcagcagact ggtgtcaccg tgcgtaccgt cctcgcgcca gctgtcgtgc cacgctccgt
     2221 attcgagttc gcgcagttca gccagcgtca tggtgctgac cagtccggcc cccgtcgagg
     2281 ttcggtccag acgccggtca tgcacgcaga ccagatggcc gtcgcgggtc aggcgcacat
     2341 cgcactcgac gccgtcggcg ccctctttga gtgcgagatc gtaggcagcc agcgtatgtt
     2401 ccggccgaga cgccgaggcg cctcggtggg caacaacaaa ggggtgccca gccagcacct
     2461 cctccgccca cgtcatccct ctatgctgcc ggttcttgcc cctccagctc aactggaccg
     2521 gcggacgaga ccgtcacagg ttggtcgtcg gtcaccacca cccatcggtg cgcaggccgt
     2581 tcgaccggtt tgctctcgaa gccctcgaat acccgcgcaa cggcggccat cgtgactccc
     2641 gcaaacagat aggcgaacac catcagcacg gtgttgttgg cgatgccctg ggcgtccgtg
     2701 gcaaagctcg tcgcgatcgc gaaaatcgaa accaggtagc tgaaagccca agcgaaccac
     2761 caccggacga tcggtgttcg tagccgggcg tagcgctctt cgatggttgc cagctcaatt
     2821 acgtacaccg gcgcccacag cagattggcc agcggcacca cacagccggc ccacaacgcc
     2881 cggactgaac gcggctccgg caggccaaga tgggcgaaca cggcggctct ccgcgcgacc
     2941 agccattgga tcagcatcac accggaggtg atcaccgccg cgatcgccgc cacactggcc
     3001 agaatcccga gccaatccgc ggcgatggcg acgatcgagt tcagcagggt gtcccggttg
     3061 atcaccagca gcacataccg cacgacgtag accagggcgg cgaaggcgag caccaacacg
     3121 ctcaggaaca atgcggtgcg gacaaccgcc gccgacggcc cggcctgcgt gggcttttcc
     3181 gctgccgccg gagcctgctc gacgagatcc gccaaacccc agcgcgggat cacggcgtag
     3241 cgcggtgtgg gacccagggg ccgtcgccca tgtcgcgaag gcggtgcggc acccggacgc
     3301 acggctatcc agcggtaacc cggcggcaac ggcgggggcg tgcgctgcca gcctcgcgcc
     3361 gccggcggcc tggcggacgg cggacggcct tgcgcgctcc accgagggtc ggccgcgggc
     3421 acgtccgcca acggcgccat cagcgccccg cgacaacgag gacaccattc gcgccgccgc
     3481 tcgcggacgt tccaccgagt gccgcactgg gagcacacct ggatcaccgg accagcctag
     3541 ctccggcacc tcaccgcgct ccggcgacga cgcgcgctgc ggcacggtga ggaattccgc
     3601 cgtcggtccg gcaacaccaa cagttgccta tggctatcca cagtttccac aggtttatcc
     3661 acagcgagcg cttgggtatg cacggcatca acactgcctt tgccggttga cttgggttgc
     3721 cgtgtggata agcgcgggtt ggtacaggcg tgccatatac acccccgatc ggtatgcgag
     3781 cgaaatgact tgcctcacta actacccaca gacggcccgg ctgtccggca cacccggact
     3841 cgagccggac atccttttcc cgtacgttcg tgcaccgcgc tgccaccccg aatccgcgcc
     3901 gtggagcgct catacgccgc gtgttttcaa cctcgccgca agcggctact atcacggtca
     3961 cggaatctca gttgtgactc acctcactcg agtgaggtga aggtgcaggt gaaaggcgac
     4021 tgatggccac gcattcgttc ggtccgggtt cgccggcctc atcgaacccg tattccggct
     4081 cgcctgcttg ggaccacagc tatagcggcg ccgccttgcg cgccgccctg atcgcgctgg
     4141 cgctactcgc ggttcttgtg gtcatcgtct tgtcttgaaa tcacgccgaa cgggtatccg
     4201 ggtcatcgcc ccctagcggt cgttcttgcg gcgcgcgcgg tcatggagca gggttgatga
     4261 cgccaggccg ccgcgtggcg ggctccgtgc gaatgcgcgt caagacgatc accaccgatc
     4321 atcgaagaaa ggaatgccgt ggctgaatac accctgcccg atttggactg ggactatgca
     4381 gcgctggaac cgcacatctc cggtcagatc aacgagatcc accacagcaa gcatcacgcc
     4441 gcctatgtga agggcgtgaa cgacgccgtc gcgaaacttg aggaggcacg cgccaaggat
     4501 gaccactctg cgatcttcct gaacgaaaag aacctggcct tccacctcgg tggccacgtg
     4561 aaccactcga tctggtggaa gaacctctcg ccgaacggtg gcgacaagcc gaccggcgag
     4621 ctcgccgccg ccatcgacga cgcgttcgga tcattcgaca aattccg
//
LOCUS       DASSGQ010000141        11683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_800391,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000141 DASSGQ010000000
VERSION     DASSGQ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11683
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800391"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VFI55_06420"
     CDS             complement(<1..260)
                     /locus_tag="VFI55_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET7073700.1"
                     /translation="MTWDFSTEPEFEKKLDWIREFVREEVEPLEVLFPGCEYRPLNDQ
                     RRRIIDPLKQQVRDNGLWAPHLGPELGGQGFGAVKLALINEIL"
     gene            complement(333..1097)
                     /locus_tag="VFI55_06425"
     CDS             complement(333..1097)
                     /locus_tag="VFI55_06425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7073701.1"
                     /translation="MGYADQLFDLADRVILITGGSRGLGREMAFAVARCGADVVIASR
                     NMDNCVSTAKEIEAETGRSAMPYQVHVGRWEQLDGLVEATYERFGKVDTLINNAGMSP
                     LYDKLTDVTEKLFDAVINLNLKGPFRLSALVGERMVAAGRGSIINVSSTGSLRPNGGI
                     IPYAAAKAGLNAMTKGLAQAFGPTVRVNTLMAGPYLTDISQAWGLENDSTNRFGHLFL
                     ERAGDPREIVGAALFLASDASSFTTGSILRADGGIP"
     gene            complement(1099..2145)
                     /locus_tag="VFI55_06430"
     CDS             complement(1099..2145)
                     /locus_tag="VFI55_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET7073702.1"
                     /translation="MTAAGETQLDPGVLGRWLDANDAPGNGEEPMLDQLKGGSQNTLF
                     VIKRGGERMVLRMPGARADAARIDGLLREIHLVRALSGTDVPHAALIAADDTGTVLGM
                     PFYVMRAIDGWSPMDGGWRAPFDTDLAARRGLAFELVEGAAKLGRVDWRAHGLGGFGR
                     PEGFHQRQVDRWLTFLDAYKVRDLPGLDEAADWLRRNRPARYKPGIMHGDYQFANVMF
                     AHGGPARLAAIVDWEMTTVGDPLLDLAWALLGYDGENPRAEGFYLDMRGMPTRSELLE
                     HYETVSGLSTENIDYYLVLANWKLGIVLEKTYAAGVRTGNVDPKITETFGAMIPQLIA
                     TAAGLARSLRTEAR"
     gene            complement(2209..2883)
                     /locus_tag="VFI55_06435"
     CDS             complement(2209..2883)
                     /locus_tag="VFI55_06435"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7073703.1"
                     /translation="MTQPQMDWDAAYRQESPPPWSIGRPQPELAVLIDQGKIRGDVLD
                     AGCGHAALSLALAARGYAVVGLDASATAVEAAAAVAAEQGLTTATFAQADVTTFRGYD
                     GRFSTILDSGLFHALPPERRQDYLQSIFRAAAPGAALYILAFAAEAFADRDDDWPGPR
                     GFTEAELRDTVSTLWRVDQVRPAKVYGNDDALASSPAAFANVEHDSEGHFMAPGLLLS
                     AHKSER"
     gene            3050..3658
                     /locus_tag="VFI55_06440"
     CDS             3050..3658
                     /locus_tag="VFI55_06440"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7073704.1"
                     /translation="MQVLLVRHALPLRSEHGEGSDPDLSEEGLAQVGRLPKALARFPI
                     ARVVSSPQRRAIQTAEPVAAARGLTVEVDDRFAEYDRDLPMYIPIEQIRDEMPEEWAR
                     LAQGHLPSAVDEEAFRARVRAAADDVVAAADPDHTVAVFSHGGVINALLHEILGTSRL
                     LSFPVDYASVTRLLFSRSGQATVGSVNGTEHVWDLLPRNQRF"
     gene            3798..4850
                     /locus_tag="VFI55_06445"
     CDS             3798..4850
                     /locus_tag="VFI55_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET7073705.1"
                     /translation="MTSADGLEGLDLPSLDRYLHSLGIGRAGELRAEFISGGRSNLTF
                     RVYDDATSWLVRRPPLHGLTPSAHDMAREYKVVAALQDTPVPVARTIALCEDDAVLGT
                     PFQIVEFVAGHVVRRRAQLEAFSRTVIDGCVDSLIRVLVDLHSVDPNAVGLADFGKPS
                     GYLERQVRRWGSQWDLVRLPEDHRDADVERLHSGLRHSVPQQSRTSIVHGDFRIDNTI
                     LDSDDPTKVRAVVDWELSTLGDPLSDAALMCVYRDPALDLIVNTQAAWTSPLLPTADE
                     LADRYSLVSGLPLAHWEFYMALAYFKLAIIAAGIDFRRRMSELAQGTGDTSEHQPEVV
                     GPLISRGLAELAKLPS"
     gene            complement(4879..5229)
                     /locus_tag="VFI55_06450"
     CDS             complement(4879..5229)
                     /locus_tag="VFI55_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HET7073706.1"
                     /translation="MSSQSPVSPGHPPAKPAQSPKKAAKSTEPAVGFTRAGALWTALI
                     AGFLILILLLIFIAQNTASTPFTFFAWHWSLPLGVAILLAAVGGGLLTVAVGTARIIQ
                     LRRVAKKHHAAASG"
     gene            5323..6270
                     /locus_tag="VFI55_06455"
     CDS             5323..6270
                     /locus_tag="VFI55_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7073707.1"
                     /translation="MRMLPRMQRMSVHTSVWLAVVCLGAACSNSDPLSAETRSPKSIV
                     VGSGDFPESEIVAEIYAQALQANGFEIGRRMGIGSRETYIPALKDHSIDLVPEYIGNL
                     LLYFAPASTATMLDAVELELYKKLPGDLSILTPSPASDTDTVTVTSETAGAWNLKTIA
                     DLAAHSPDVKFGAPSAFERRPSGLPGLRQKYSLDISPGNFVAINDGGGAVTVRELVEG
                     RVTAANIFSTSPAIPQNQLVALDDPEHNFLAGNIVPLVNSQKKSDLLKDVLDAVSAKL
                     TTPGLAGLNAAVSGNSGIDPDQAARNWVRDNGFNHPIGQ"
     gene            6305..7420
                     /locus_tag="VFI55_06460"
     CDS             6305..7420
                     /locus_tag="VFI55_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7073708.1"
                     /translation="MIVFDDVSKVFADGTTAVDQLSLTVPNGKLTVFVGSSGSGKTTA
                     LRMINRMVEPTHGTITVDGADVAGVNPVQLRLGIGYVIQHAGLMPHQRVIDNVATVPV
                     LKGQSRRDARKAAYEVLERVGLDARLATRYPGQLSGGEQQRVGVARALAADPPILLMD
                     EPFSAVDPVVRHELQNEILRLQSELHKTIVFVTHDIDEALKLADRVAVFGRGGSLQQY
                     DEPAQLLSRPANEFVSRFIGLGRGYRWLQLIDADGLPLHDVERIAADNLSDSGLPDGW
                     AVVVDDAGAPMGWIDAESLRRHRAGASLPDSMNGVGSLFRPGGNLSQALDAALSAPSG
                     MGVAVDDGGKVIGGVLAADVLAAVAARRQGPEGPRGN"
     gene            7425..8111
                     /locus_tag="VFI55_06465"
     CDS             7425..8111
                     /locus_tag="VFI55_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7073709.1"
                     /translation="MHYLFNHLDDAWALTIVHLRLSLMPVLIGLAIAVPLGALVQRAP
                     IARRLTTATASVVFTIPSLALFVVLPTIIGTRILDEANVMVALTAYTAALLVRAVLEA
                     LDAVPAQVCDAATALGYPTIKRMLKVELPLSIPILVAGLRVVVVTNIAMVSVGSVIGI
                     GGLGTWFTEGYQTDKSDQIVAGIIALFLLAIVIDVLIMLAGRLATPWERAGRATRRRS
                     VIAPVVGGAR"
     gene            8108..8800
                     /locus_tag="VFI55_06470"
     CDS             8108..8800
                     /locus_tag="VFI55_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7073710.1"
                     /translation="MNFLQQALSYLLTVDHWTGPVGLAARTLEHLEYTVIAVGASALI
                     AVPVGLLIGHTGRGTLLVVGAVNGLRALPTLGVLLLGVLLFGLGAGPPLLALMLLGVP
                     SLLAGTYAGVANVDRTAVDAARAMGMTEAQLLLRVEVPNAMPMILSGLRNATLQVVAT
                     ATVAAYASLGGLGRYLIDGIKEREFQLALVGALMVAALALILDGLLALAVWVSVPGTG
                     RLPSAIVPQHNE"
     gene            8861..9460
                     /locus_tag="VFI55_06475"
     CDS             8861..9460
                     /locus_tag="VFI55_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HET7073711.1"
                     /translation="MKAASSDPTRRVWQAMLTWRAQDVSRMESVRVQLSGKRIRANGR
                     IVAAATAINPAFGAYYDLQTDETGATKRFGLTVTLAERERQLAIARDEENMWLITDHQ
                     GERRAGYNGALDVDVVFSPFFNALPIRRLSLHERADSVTLPMVYVNVPEMAVTAATVS
                     YCSEGRLDGIKLHSPVADTTVSVDEDGFILDYPSLAERI"
     gene            complement(9423..10367)
                     /locus_tag="VFI55_06480"
     CDS             complement(9423..10367)
                     /locus_tag="VFI55_06480"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HET7073712.1"
                     /translation="MCVLGLGLIGGSIMRATKAAGREVFGYNRSVEGAHAAIADGFEA
                     SINLTDTLIRAADTDALIVLAVPMPALGTMLTHISELAPGCSLTDVTSVKAAVLDEVA
                     TAGLRERFVGGHPMTGTVHSGWAAGYAGLFIRTPWVVSVDDGVDATVWSKVMTLALDC
                     GALVVPAKSDEHDAAAAAISHLPHLLAEALAVAAAEVPLAFALAAGSFRDGTRVAGTA
                     PDLVRAMCEGNSAQLLPALDRAIKLLSGARESLGSVNSVADLVEAGHAARTRYDSFPR
                     FDIVTVAIGAEDWREELAAAGRAGGVIRSALPSLGNPR"
     gene            10489..11010
                     /locus_tag="VFI55_06485"
     CDS             10489..11010
                     /locus_tag="VFI55_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003899676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine deaminase-associated protein"
                     /protein_id="HET7073713.1"
                     /translation="MGAQRASAQGPSADVPDGFGVAVVREEGQWRCSALATKSLTSLA
                     AAETELRELRSAGAVFGLLDVDDEFFVIVRPAPSGTRLLLSDATAALDYDIAAEVLDN
                     LDADIDPEDLEDAEPFEEGDLGLLSDIGLPEAVLGVILDETDLYADEQLGRIAREMGF
                     ADQLSAVIDRLGR"
     gene            11083..11541
                     /locus_tag="VFI55_06490"
     CDS             11083..11541
                     /locus_tag="VFI55_06490"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET7073714.1"
                     /translation="MIADEDLIRTALSVAATAGPRDVPIGAVVVGADGTELARAVNAR
                     EALGDPTAHAEILAIRAAASVLGDGWRLEGTTLAVTVEPCTMCAGALVLARVARLVFG
                     AWEPKTGAVGSLWDVVRDRRLNHRPEVRGGVLAQECAAPLEAFFARQRLG"
     gene            11571..11660
                     /locus_tag="VFI55_06495"
     tRNA            11571..11660
                     /locus_tag="VFI55_06495"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11605..11607,aa:Ser,seq:cga)
BASE COUNT         1893 a         3808 c         3966 g         2016 t
ORIGIN      
        1 agaatctcgt tgatcagcgc cagcttgacc gcgccgaagc cctggccacc cagttcgggg
       61 cccaaatgcg gcgcccacaa gccgttgtcg cggacctgct gtttgagtgg gtcgataatg
      121 cggcgccgct ggtcgttcag cggtcggtac tcgcagcccg ggaacagcac ctccagaggt
      181 tccacctctt cgcgcacgaa ctcgcggatc cagtccagct tcttctcgaa ctccggttcg
      241 gtggagaagt cccaagtcat tcgaatgctc ctactcgttc attggtggcg acccgttgcg
      301 cccgcctccg ccgcgctttt gcgatcgcca cgtcagggga tcccgccgtc ggcccgcagg
      361 attgaacccg tagtgaaact ggacgcgtcg gacgccaaaa acagtgcggc gccgacaatt
      421 tcgcgcggat cgccggcgcg ttccaagaaa agatgaccga acctgttggt gctgtcgttt
      481 tccagacccc aggcctgact gatgtcggtc aggtagggcc cggccatcag tgtgttgacc
      541 cgcacggtgg gtccgaacgc ctgtgccagg cctttggtca tggcgttgag cccggccttg
      601 gccgcggcat agggaatgat gccgccgttc gggcgcagcg acccggtgga actcacgttg
      661 atgatggacc cgcggcccgc ggccaccatc cgctcgccca ccaatgcgga taaccggaag
      721 ggccccttga ggttcaggtt gatcaccgcg tcgaacagct tctcggtgac gtcggtcagc
      781 ttgtcgtaca gcggcgacat gcccgcgttg ttgatcaacg tgtcgacctt gccgaaccgc
      841 tcgtaggtcg cctcgaccaa tccgtcgagc tgttcccaac gtccgacgtg tacctgatag
      901 ggcatggcgg agcggccggt ctccgcctcg atctccttgg cggtggacac acagttgtcc
      961 atgttccggc tggcgatcac cacgtcggcg ccgcagcgcg cgacggcgaa ggccatttcg
     1021 cgtcccaggc cacggctgcc gccggtgatc aatatcactc ggtcggcgag gtcgaaaagc
     1081 tggtccgcgt aacccatttc agcgggcctc ggtccggaga gagcgcgcca gcccggctgc
     1141 cgtggcgatg agctgcggga tcatcgctcc gaaggtttcg gtgatcttgg ggtcgacgtt
     1201 cccggtgcgc accccggccg cgtaggtctt ctcgagcaca atgcccagtt tccagttggc
     1261 gagcaccagg tagtagtcga tgttctcggt ggagagccca ctgacggtct cgtagtgctc
     1321 gagcagttcg ctgcgggtcg gcatgccgcg catatccagg tagaaccctt cggcgcgcgg
     1381 gttttcgccg tcgtagccca gtagcgccca ggcgagatcc agcagcggat cgccgacggt
     1441 ggtcatctcc cagtccacga tcgcggctag tcgagccggc cccccgtgcg cgaacatcac
     1501 gttggcgaac tgatagtcgc cgtgcatgat gcccggcttg taccgcgccg gccgattgcg
     1561 gcgcagccag tcggcggcct cgtcgaggcc gggcagatcg cgcaccttgt aggcgtcgag
     1621 aaacgtcaac cagcgatcga cctgtcgctg gtggaatcct tccggacggc caaagccgcc
     1681 gagcccgtgg gcgcgccaat ccacccgccc cagcttggcg gcgccttcga cgagttcgaa
     1741 cgccagcccg cgccgcgcgg ccagatcggt gtcgaagggt gcccgccatc cgccgtccat
     1801 cgggctccac ccgtcgatcg cccgcatcac gtagaacggc atgcccagca ccgtgccggt
     1861 gtcgtcggcg gcgatcagtg ccgcgtgggg cacatcggtg ccggacaggg cgcgcaccaa
     1921 gtggatttca cgcagcaagc cgtcgatgcg ggcggcgtcg gcgcgggcgc cgggcatccg
     1981 cagcaccatg cgttcgccgc cgcgcttgat gacaaacagg gtgttctgcg agccgccctt
     2041 gagctgatcc agcatgggtt cttcgccgtt gcccggtgcg tcgttggcat ccagccagcg
     2101 cccgagaaca cccgggtcca gttgggtttc gccggcagcc gtcattgtct gcacaccccg
     2161 ctctccacac agagaatagc attctccaca gggccgggaa gcgctatgtc accgctcgga
     2221 cttgtgcgcg ctgagcagca accccggcgc catgaagtgg ccctcgctgt catgttcgac
     2281 gttcgcgaag gcagcggggg aggacgcaag cgcgtcgtcg ttgccgtaga ccttcgcggg
     2341 gcgcacctga tcgacacgcc aaagcgtcga gacggtgtcg cgcaactcgg cctcggtgaa
     2401 gccacgcggg ccgggccagt cgtcgtcccg gtcagcgaat gcttcggcgg cgaaggccag
     2461 gatgtagagc gccgcccccg gcgcggcagc gcgaaagatc gactgcagat agtcctggcg
     2521 cctctcgggc ggcagggcgt ggaacagtcc gctgtccagg atggtggaga agcggccgtc
     2581 atagccacgg aaggtggtga cgtcagcctg cgcgaaagtc gccgtcgtca gtccttgttc
     2641 ggcggcgacc gcggccgcgg cctcgaccgc ggtcgcgctg gcgtccagcc cgacaactgc
     2701 gtagccgcgc gccgcgaggg ccagcgacaa cgcggcgtgg ccacagccgg cgtccagcac
     2761 atcgccacgg atcttgcctt gatcgatgag caccgccagc tcgggctgcg gccggccgat
     2821 gctccacggc ggcggtgatt cttgccggta ggcggcgtcc caatccatct gcggctgggt
     2881 cattctgtcc tcctccagat cgggcgttgt tttcagcgac gccattctgc ccgcgttgcc
     2941 gaccacacac gacgcgcact gggtcgcacc gctaccagca caatggaaaa ctggcggatc
     3001 gggcggggcg tcaagccctg gttctgctta cccgacgaca ggaagaaaca tgcaagtgtt
     3061 gctggtccgg catgcgctac cgctgcgcag cgaacacggc gagggctccg acccggacct
     3121 gtcggaggaa gggttggcgc aggtcggacg actgcccaag gcgctggcca ggtttcccat
     3181 cgctcgggtg gtgagcagcc cgcagcgccg cgcgatccag accgccgaac cggtggcagc
     3241 ggcgcgcggg ctgaccgtcg aggtcgacga tcgattcgcg gaatacgacc gtgaccttcc
     3301 gatgtacatc cccatcgagc aaatccgcga cgaaatgccc gaggaatggg cgcggttggc
     3361 tcaagggcat ttgcccagcg cggtagacga ggaggcgttt cgcgcccgcg tccgtgcggc
     3421 agccgacgat gtggtcgcgg cggccgatcc cgatcacacc gtggcggtgt tcagccacgg
     3481 tggggtgatc aacgcgctgc tgcacgagat ccttggtacg tcccggctgc tgtcctttcc
     3541 tgtcgactac gcgtcggtga cccggctctt gttctcgcgg tccggtcagg cgacggtggg
     3601 gagcgtcaac ggcaccgagc acgtgtggga cctgttgccc cgaaaccagc ggttctagga
     3661 gtcgcgcgcc tcgggtctgc gtccagcacg tgctgatcgc ccgtaccgca gcgccaccgc
     3721 agagtcgaag ccgtgggcgc agagtccaga cgggcagctg ttgcgcgccg ggaaatcggc
     3781 ggtggtaaac aagtccggtg acttcagctg atggactcga agggctcgac ctgccctcgc
     3841 tggaccggta cctgcattcg ctcggtatcg gacgcgccgg cgagttgcgg gccgagttca
     3901 tctctggtgg ccgctcgaac ctgaccttcc gggtgtacga cgacgcgacc agctggctgg
     3961 tgcgacgccc gccgctgcat gggctgacgc cgtcggcgca cgacatggcc cgtgaataca
     4021 aggtcgttgc cgcgctgcag gacacgccgg ttccggtcgc gcgcacgatc gcactgtgtg
     4081 aggacgatgc ggtgctgggc acgccctttc agatcgtcga attcgttgcc gggcacgtgg
     4141 tacgccggcg tgcccaactg gaagcgttca gtcgcaccgt gatcgacggc tgtgtcgatt
     4201 ccctgatccg ggtactcgtc gatctgcaca gtgtcgaccc aaacgccgtg gggctggccg
     4261 attttggcaa gcccagcggt tatctcgagc gtcaggtgcg ccgatggggt tcgcaatggg
     4321 atctggtccg gctgccggaa gaccaccgcg acgccgacgt tgagcgactg cattcgggcc
     4381 tgcgccactc ggttccgcag cagagccgga cgtcgatcgt gcacggtgac ttccggatcg
     4441 acaacaccat cctggactcc gacgatccga ccaaggttcg cgccgtcgtc gattgggagc
     4501 tttcgacatt gggggacccg ctcagcgatg cggccctgat gtgcgtgtac cgcgatccgg
     4561 cgctggacct gatcgtcaac acgcaagctg cctggacttc gccgctgttg ccgacggcag
     4621 atgaactggc agaccgttac tcgctggtgt ccggtttgcc gctggcgcac tgggagttct
     4681 atatggcgtt ggcttacttc aagctcgcca tcatcgccgc gggtatcgat ttccgtaggc
     4741 ggatgtcgga gctggcacag gggacgggcg acacgtcgga gcaccagccc gaggtggtgg
     4801 ggcccttgat ctcccgcgga ctggcggagc tggccaagct gcctagttga gcgccgtcac
     4861 ggacgcaatg gcgtcccgct agcccgaagc cgccgcgtgg tgtttcttgg cgacacgacg
     4921 tagctggatt atccgcgcgg tgccgaccgc cacggtgagc agcccgccgc ccactgccgc
     4981 cagcagaatg gccacgccca gcggcaggct ccagtgccag gcaaagaacg taaacggggt
     5041 cgacgccgtg ttctgagcta tgaagatcag caacaggatc aggatcagga agccggcgat
     5101 caatgcagtc cacaaagcgc cggcgcgggt aaagccgacg gccggttctg tggattttgc
     5161 ggccttcttg ggcgattgcg ccggcttggc gggcgggtga ccgggggaga caggggactg
     5221 gctgctcata gtgccatctt tgtcccatct cgcccggaat gaaaccgaac cacgcaggaa
     5281 accttgcggc gccccggcgt ttctgccgtt agtgtcggcg gtatgaggat gctgccgcgc
     5341 atgcaacgca tgtcggtcca cacctccgtg tggttggcgg tggtgtgcct gggtgcggca
     5401 tgcagcaatt ccgatccgct gagtgcggag acccgcagcc cgaaatcgat cgtggtcggc
     5461 tccggcgact ttcccgaatc ggagatcgtc gccgaaatct acgcacaagc tttgcaagcc
     5521 aacggcttcg aaatcgggcg gcggatggga atcggcagca gggagacgta tatcccggcg
     5581 ctcaaagatc attccatcga cttggtgccg gagtacatcg gcaacctgct gttgtacttc
     5641 gcgcccgctt ccacggcgac catgctcgat gcagtcgagt tggagctcta caagaaactg
     5701 cccggtgacc tgtcgatcct gacgccgtcg ccggcctccg acaccgatac ggtgaccgtc
     5761 accagcgaga ccgccggcgc atggaacctg aagacgatcg ccgacttggc ggcacactcg
     5821 cccgacgtga agttcggagc accctcggcg ttcgagaggc ggccttccgg gctgcccggg
     5881 ttgcgccaga agtattctct cgacatcagc ccgggcaact tcgttgccat caacgacggc
     5941 ggcggggcgg tgacggtgcg cgagttggtg gagggcaggg tcaccgccgc caatatcttc
     6001 agcacgtcac cggctatccc gcaaaatcag ctggtggcgc tcgatgaccc ggagcacaac
     6061 ttcctggccg gcaacattgt gccgctggtg aattcgcaga aaaagtcgga cctgctcaaa
     6121 gacgtgctgg acgccgtgtc ggcgaaactg accacgcccg gcctggccgg actcaacgcc
     6181 gcggtatcgg gcaactccgg catcgatccc gaccaagcag cgcgaaactg ggtgcgcgac
     6241 aacggcttca accatccgat cgggcaatga tggggctgca tgatgcggta ggcggacaac
     6301 cgcgttgatc gtcttcgacg atgtcagcaa ggtattcgcc gacgggacca ccgccgtcga
     6361 ccagctgagc ctgaccgttc ccaacggcaa gttgacggtc ttcgtcgggt cctccggcag
     6421 cggcaagacc accgcgctgc ggatgatcaa tcggatggtc gagccgaccc acggcaccat
     6481 aaccgtcgac ggggcagacg tggcgggcgt caacccggta cagctgcggc tcggcatcgg
     6541 ctacgtcatc cagcacgcgg gcctgatgcc ccatcagcgc gtgatcgaca acgtcgcaac
     6601 ggttccggtg ctgaaggggc agtcccgccg ggacgctcgc aaagccgcct acgaggtgct
     6661 cgaacgcgtc gggctggacg ccaggctcgc cacgcgttac cccgggcagc tttccggcgg
     6721 tgaacaacaa cgcgtcggcg tggcacgcgc tctggccgcc gatccaccca tcttgttgat
     6781 ggacgagccg ttctcggccg tcgacccggt ggttcgtcac gagctgcaga acgaaatatt
     6841 gcgcctgcaa agcgaattgc acaaaaccat cgtcttcgtc acgcacgaca tcgacgaggc
     6901 gctcaagctt gccgaccgcg tggcggtgtt cgggcgcggc ggctcgctgc agcagtacga
     6961 cgaacccgcg cagctgctct cgcggcctgc caatgaattc gtgtccaggt tcatcggtct
     7021 gggccgcggt tatcgatggt tgcagctcat cgacgcggac gggctgccac tgcacgacgt
     7081 cgagcggatc gcagcggaca acctctccga ctcgggactt cccgacggct gggcggtggt
     7141 ggtcgacgac gcgggggcgc cgatgggctg gatcgacgcc gagagcctgc ggcggcatcg
     7201 tgccggtgcg tcgttgcccg acagcatgaa cggcgtcggc tcgctgttcc gtcccggggg
     7261 caacctcagt caagcgctgg acgcggcgct gtcggccccg tccgggatgg gtgtggccgt
     7321 tgacgacggg ggcaaggtga tcggcggcgt actggccgcc gacgtgctcg cggcggtggc
     7381 ggcccggcgg cagggcccag agggacccag agggaattag gcagatgcac tatctgttca
     7441 accacctcga cgacgcctgg gcgctgacta tcgtccatct tcggctgtcg ctgatgccgg
     7501 tgctgatcgg attggcgatc gcggtgccgc tgggcgcgct ggtgcaacgc gcccccattg
     7561 cccgacggtt gacgaccgcg accgccagcg ttgtgttcac catcccgtcg ctggcgctgt
     7621 tcgtagtgtt gcccacgatc atcgggaccc ggatcctcga cgaggccaac gtcatggttg
     7681 cgctgacggc ctacaccgcc gcgctactgg tccgtgcggt gctcgaggcg ctggacgcgg
     7741 tgccagccca ggtgtgcgac gcggccaccg cgctcggcta cccgacgatc aaacggatgc
     7801 tcaaagtcga gctgccgctg tctatcccga tactggtcgc cgggttgcgc gtggtcgtgg
     7861 tgaccaacat cgcgatggtg tcggtgggtt cggtgatcgg catcgggggc ctgggcacct
     7921 ggtttaccga ggggtaccag accgacaaga gcgatcagat cgtggccggc atcatcgcct
     7981 tgttcttgct cgcgatcgtc atcgacgtgc tcatcatgct ggctggccgg ctggccacgc
     8041 cctgggaacg cgccgggcgc gccacccgac gacggtcggt gatcgccccg gtcgtgggcg
     8101 gcgcgcgatg aacttcctgc aacaggcgct gtcctacctg ctgaccgtgg accactggac
     8161 cggtccggtc gggcttgccg cgcgcacctt ggagcatctc gaatacaccg tcatcgcggt
     8221 gggcgcgtcg gcgctgatcg cagtcccggt cgggctgctc atcgggcaca ccggcagggg
     8281 cacgctgttg gtggtgggtg cggtcaacgg gctgcgcgca ctgccgacgt tgggggtgct
     8341 gctgctgggg gtgctgttgt tcgggcttgg tgcggggcca ccgctgcttg ccctgatgct
     8401 gctgggtgtt ccgtcgctgc tggccggcac ctacgcgggc gttgccaatg tcgaccgaac
     8461 ggcggtcgac gcggcccgcg cgatgggaat gacggaggcc cagttgctgc tgcgcgtcga
     8521 ggtgccgaac gcaatgccga tgatcctcag tgggctgcgc aacgcgacgc ttcaggtggt
     8581 cgccaccgcg acggtggcgg cctacgccag cctcggcggc ctgggccggt atctgatcga
     8641 cgggatcaag gagcgcgaat ttcaactcgc cctggtcggc gcgctgatgg tggcggcgtt
     8701 ggcactgatc ctcgacggcc tgttggcgtt ggcggtatgg gtttcggtgc cgggcaccgg
     8761 ccggttaccc tcggcgatcg tcccgcagca caacgagtga tgcttagcgc agttacgaac
     8821 aaccatgcgg tggtcggcgg acacgtctta cggtagatat gtgaaggcag cttcctctga
     8881 tccgacccgg cgcgtgtggc aggcgatgtt gacctggcgt gcacaggacg tctcccgaat
     8941 ggaatcggta cgagtccagc tgtccggcaa acggatcagg gccaacgggc ggatcgtggc
     9001 cgcggccacc gcaattaatc ccgcgttcgg cgcctactac gacctgcaga ccgacgagac
     9061 cggcgccacc aagcgcttcg ggttgaccgt gacgctggcc gagcgggaac gtcagctcgc
     9121 cattgcccgc gacgaggaaa acatgtggtt gattaccgac caccaaggtg agcggcgcgc
     9181 gggatacaac ggcgccctcg acgtcgacgt cgtgttcagc ccgttcttca acgccctgcc
     9241 gatcaggcgc ctgtctctgc acgaacgggc ggactcggtc acgctgccga tggtctacgt
     9301 gaacgtaccg gagatggccg tcaccgcggc cactgtcagc tactgcagcg agggacgcct
     9361 cgacggcatc aagttgcact ctccggtcgc cgacaccacg gtgagcgtcg acgaagacgg
     9421 gttcatcttg gattacccaa gcttggcaga gcggatctga tcaccccgcc cgcccggccc
     9481 gctgcggcca gttcctcgcg ccagtcctcc gcgccgatgg cgacggtgac gatgtcgaag
     9541 cgcgggaagc tgtcgtaccg tgttcgcgcg gcgtgaccgg cctcgacgag gtcggccacg
     9601 gagttgacag aacccagcga ctcgcgggca ccgctgagca acttgatggc ccggtcgagc
     9661 gccggcagca gctgcgccga gttgccttcg cacatcgccc gcaccaggtc gggggccgtg
     9721 cccgccaccc gggtgccgtc gcggaacgac cccgcggcca gcgcgaaggc cagcggaacc
     9781 tctgcggcgg cgacggccag cgcctcggcg agcaggtgtg gcagatgtga gatggccgcg
     9841 gccgcggcgt cgtgctcgtc ggatttggcc ggtaccacca gtgcgccgca gtccagcgcc
     9901 aacgtcatca ccttcgacca gacggtcgcg tccaccccgt cgtccacgct gaccacccac
     9961 ggcgtcctga tgaaaagacc ggcgtatccg gccgcccaac ccgagtgcac ggtgcccgtc
    10021 atcgggtgac cgccgacgaa gcgttcgcgc agaccggcgg tggccacctc gtcgagcacc
    10081 gcggctttga cgctggtgac gtcggtcagc gagcatccgg gcgccaattc gctgatgtgg
    10141 gtgagcatgg ttcccaacgc cggcatcggc acggccagca caatcaatgc gtcggtgtca
    10201 gcggcgcgga tcagcgtgtc ggtgaggttg atgctggcct cgaacccgtc ggcgatggcc
    10261 gcgtgtgcgc cctccaccga ccggttgtag ccgaatacct cacggccggc ggccttggtg
    10321 gcccgcatta tcgagccgcc gatcaaaccc agtccaagca cgcacaaagg tgtccccgcc
    10381 acagtcctag gtttgcacag tttgacggcc cacggtgcgt tcggatgccg ttgctcctcg
    10441 tacgtttgtc tggtcaaggt gcctggtcag cgactagcgt aggcgcccat gggagcacag
    10501 cgggcgtctg cgcagggccc gtccgccgac gtaccggacg gcttcggcgt cgcggtcgtg
    10561 cgtgaagagg gccaatggcg ctgttctgcg ctggccacca aatcgctgac gagcctggcg
    10621 gccgccgaga cggaattacg tgaactgcgg agcgcggggg cggtttttgg acttctcgac
    10681 gtcgatgacg agtttttcgt catcgtgcgc ccggcgccgt ccgggacccg gctgctgttg
    10741 tcggatgcca ccgccgcact cgactacgac atcgccgccg aagtgctgga caacctggac
    10801 gccgacatcg accccgagga cctcgaggac gcggaaccct tcgaggaggg cgacctgggg
    10861 ttgctgtccg acatcgggct gcccgaggcg gtgttggggg tcatcctcga tgaaaccgat
    10921 ctctatgccg acgagcagtt gggccgcatc gccagagaga tgggtttcgc cgatcagctg
    10981 tcggcggtca tcgaccgcct cggtcggtga tcgcaagcgc aggtcgccgt cacaggtccg
    11041 gtcggtgatc gcgagcgcgg gtcgccgcca caggcccggt cggtgatcgc cgacgaagat
    11101 ctgatccgta cggcactgtc ggtggccgcg acggccggcc cgcgcgacgt gcctatcggc
    11161 gcggtggttg tcggcgccga cggaaccgag ctggcccggg cggtgaatgc ccgtgaggcc
    11221 cttggtgatc cgacggctca cgccgaaatc ctggcgatac gggcggcggc gagcgtgctc
    11281 ggagacgggt ggcgactgga ggggaccaca ctcgccgtga ccgtcgaacc gtgcaccatg
    11341 tgcgcgggcg cactggtgct ggctcgcgtc gcgcggctgg tgttcggagc atgggagccc
    11401 aaaaccgggg cggtcggatc gctgtgggac gtggtccgcg accgtcgact caaccaccgc
    11461 ccggaggtgc gcggcggtgt gctcgcgcaa gagtgcgccg cgccgctcga ggcattcttc
    11521 gcccgccagc gattggggtg accggcccct ggttcggtaa gctgctcggc ggtggcgtgt
    11581 ccgagcggcc taaggagcac gcctcgaaag cgtgtgacgg ctaacaccgt ccgagggttc
    11641 aaatccctcc gccaccgcca aagctgtaca gatgtttgcg cag
//
LOCUS       DASSGQ010000142         3559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_435709,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000142 DASSGQ010000000
VERSION     DASSGQ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435709"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFI55_06500"
     CDS             complement(<1..421)
                     /locus_tag="VFI55_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073715.1"
                     /translation="MTGWAIKVGGPHEYAGDDAVGVPPACGGRSDEEERRMSKSAGEY
                     LSSGPRFAEGEVIERIESFRRRRPRLLDDHVTLAHGAGGKASAALLDAVFLEAFRNPL
                     LESLGDSAVLTLPSGERLAMSTDSFVVQPRRFPGGSIG"
     gene            complement(384..1055)
                     /locus_tag="VFI55_06505"
     CDS             complement(384..1055)
                     /locus_tag="VFI55_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET7073716.1"
                     /translation="MSERDEPTNFLYPFIDAQEDDAESLLADLAASAQAKATESLALR
                     RSTLEANAELLDEAATEMARRFASGGRLFTFGNGGSCTDSTTLARLFARPPLGNPLPA
                     WSLTADQAILTALGNDVGFELVFARQLIARAKAGDIAIAMSTSGNSPNLLTALREARQ
                     RGLYTIGFAGYDGGAFADNPNVDACFVVRSQSVHRIQESQALLGYQLWLTVHDRLGHQ
                     SWGAA"
     gene            complement(1055..1777)
                     /locus_tag="VFI55_06510"
     CDS             complement(1055..1777)
                     /locus_tag="VFI55_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase assembly protein HupF"
                     /protein_id="HET7073717.1"
                     /translation="MTTTAIDRGLGAELAEDLAATAFTLAKRFAAGATMWSIAPSWEP
                     HALHIAVEFVHPVIMGKRALPAVALTGPDLVDLVRVSVRPGDIVVAVAGADDTQVRSV
                     MRRSPAWGATTIWIGSGERPTAGRADHVLWLDDPDPRVPATGGFVLFYHLLWELTHVC
                     FEHSGLLKPECTEQVCVTCSDEGRLGEVVTATADGMAAGTATVRTARGVENVVTTLID
                     PVAAGELVVVHAGTAICRMEDA"
     gene            complement(1774..>3559)
                     /gene="hypF"
                     /locus_tag="VFI55_06515"
     CDS             complement(1774..>3559)
                     /gene="hypF"
                     /locus_tag="VFI55_06515"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HET7073718.1"
                     /translation="FRPAGATQGGERTQASDAALAATQGALAAGVVVAIKGIGGYHLA
                     CAVDHNGAVRELRSRKARGAKPFAMLVRDLDVARRYAHVDAAEAVVLSSPARPIVLLR
                     RRRGAPVADAVAPGSPLLGLMLPYSPIHHLLLAPVPGAAERVPDALVLTSANRSDEPI
                     CFTDDDARKRLSVLCDAVLDHNRPIHVPCDDSVVRVVRDREGARELPIRRSRGYAPLP
                     VDLGRDGPAVLAVGGELKNTFCLTDGSRAYMSGHIGDMASWETLRAFERAVRQLGEMR
                     NAPTRMAADLHPGYHTRSWAERHAGDRPLDLVQHHHAHVVSLLAEHGRIGEPIVGVSF
                     DGTGYGCDHSIWGGEILALGTDSHRFVRVGHLLPVPLPGGDAAVRNPWRMALSQLWMA
                     DIEWTPDLAPVAAATANELRLTRSQLESGTGCVPCSSMGRLFDAVASLLGVRHRIDYE
                     GQAAIELEALAESALGQATGPAGLAPSLPLTVRADGVIDPATMVQTMVAALHAGTQPA
                     PLAAAFHEAIADAVAKVVGQVAGRVRLVGLTGGVFQNVLLLRACRKRLQLNGFEVLTH
                     HTVPPNDGGLALGQAAISMLTALEEGRP"
BASE COUNT          559 a         1275 c         1200 g          525 t
ORIGIN      
        1 cgccgatgga gccgccggga aatcgcctgg gctgcaccac gaatgaatcg gtggacatcg
       61 ccaggcgttc gccgctgggc aacgtcagga ccgcactatc gcccagcgac tccagcagcg
      121 gattgcgaaa cgcctccagg aacaccgcgt ccagcagtgc cgccgaggcc ttcccgcccg
      181 cgccgtgggc cagggtcacg tggtcgtcga gcagccgcgg gcggcgcctg cggaacgact
      241 cgatgcgctc gatgacctcg ccttcggcga accgcggccc cgaggacagg tattcgcccg
      301 ctgacttgct catgcgccgc tcctcctcat cgctacgtcc cccgcaagcg ggcggtaccc
      361 ccactgcatc gtcgccggcg tactcatgcg gccccccaac tttgatggcc cagccggtca
      421 tgcacggtca accacagctg atagcccagc agcgcctggg actcctggat gcggtgcacg
      481 ctttgcgaac gaacgacgaa gcaggcgtcc acgttcggat tgtccgcgaa ggccccgccg
      541 tcgtagccgg cgaaaccgat tgtgtacagg ccgcgttggc gcgcctcgcg cagcgcggtg
      601 agcaggttgg gcgaattgcc gctggtcgac atggcgatcg cgatgtcgcc cgccttggcc
      661 cgggcgatca actggcgggc gaacaccagc tcgaacccga cgtcgttgcc cagggcggtc
      721 aagatcgcct ggtcggcggt caacgaccac gcgggtaacg gatttcccag cggcggccgc
      781 gcgaacagcc tcgccaaagt ggtggaatcg gtgcagctgc cgccgtttcc gaaggtgaac
      841 agccgcccgc ccgacgcgaa ccggcgcgcc atctcggtgg cggcctcatc cagcagctcg
      901 gcgttggctt ccagcgtgga gcgccgcagc gccaggcttt cggtggcctt tgcctgcgcc
      961 gacgcggcca ggtcggccag cagcgactcg gcgtcgtcct cctgtgcgtc gatgaacgga
     1021 tacaggaaat tggtgggctc gtcgcgctcg ctcatcaggc gtcctccatt cggcagatcg
     1081 ccgtcccggc gtgcaccacc accagttcgc cggcggccac cgggtcgatc agcgtggtca
     1141 ccacgttctc tacgccccgc gcggtgcgca ccgtggctgt tccggcggcc atcccgtcgg
     1201 cagtggccgt caccacctca cccaggcgac cctcgtcgct gcacgtgaca caaacttgtt
     1261 cggtgcactc gggtttcagc aatccggagt gctcgaaaca cacgtgggtc agttcccaca
     1321 acaggtgata gaacagcacg aagccaccgg tcgcgggcac tcgcggatcg ggatcgtcga
     1381 gccacagcac gtggtcggcc cgccctgccg tggggcgttc accgctgccg atccaaatcg
     1441 tggtcgctcc ccacgccggc gaacgacgca tcaccgagcg cacctgcgtg tcgtccgcac
     1501 cggcgaccgc gacgacgatg tcgccggggc gcacggagac ccgcaccaga tccaccaggt
     1561 cgggtccggt caacgccacg gcgggcaatg cccgtttgcc catgatcacc ggatgcacga
     1621 actcgaccgc gatgtggagc gcgtgcggct cccacgacgg cgcgatcgac cacatggtgg
     1681 cacccgcggc gaaacgcttg gccagcgtga aagcggtggc ggccaggtcc tcggccagtt
     1741 cggcgccgag cccccggtcg atcgcggtgg tggtcatggt cggccctcct cgagggcggt
     1801 gagcatcgag atcgcggcct gacccagcgc cagcccaccg tcgttcggcg gcaccgtgtg
     1861 gtgggtcagt acctcaaatc cgtttagctg caaccgctta cggcatgctc gcagcagcag
     1921 cacgttctgg aacaccccgc cggtgaggcc caccagccgg actcggccgg ccacctgacc
     1981 gacgaccttg gcgacggcgt ccgcgatcgc ctcgtggaac gccgcggcca gcggcgcggg
     2041 ttgggtgccg gcgtgcaggg ccgccaccat cgtttgcacc atggtggcgg ggtcgatcac
     2101 cccgtctgcc cgcaccgtca gcggcagtga cggcgccagc ccggcgggcc cggtggcctg
     2161 tccgagggca gactcggcga gtgcctcgag ttcgatggcc gcctgccctt cgtagtcgat
     2221 ccggtgccgc accccgagca gcgaggcgac ggcgtcgaac agccggccca tgctcgaaca
     2281 cggcacacac cccgtcccgc tttccaattg cgaacgggtg agccgcaatt cgtttgcggt
     2341 cgcggcggcc accggcgcga ggtccggagt ccactcgatg tcggccatcc acaactggga
     2401 cagcgccatc cgccacggat tgcgcaccgc cgcatcaccg cccggcaacg gcaccggcaa
     2461 caggtggccg acgcgaacga atcggtggct gtcggttccg agagccagga tttctccgcc
     2521 ccagatgctg tggtcgcagc cgtaaccggt tccgtcgaag gagacaccga cgatgggctc
     2581 gccgatgcgc ccgtgctcgg ccagcagcga gaccacatgc gcgtggtggt gctggaccag
     2641 gtccagcggc cgatcgcccg catgccgttc ggcccagctt cgggtgtggt atccgggatg
     2701 cagatcggcg gccattcgcg ttggcgcgtt gcgcatttcg ccgagctggc gcaccgcgcg
     2761 ctcgaacgcg cgcaacgtct cccaggaagc catgtcgccg atgtgaccgg acatgtaggc
     2821 acgtgacccg tcggtgaggc agaaggtgtt cttcagttcc ccgcccacgg cgagcacggc
     2881 gggaccgtcg cgccccaagt cgacggggag cggcgcatac ccccgggacc ggcggatcgg
     2941 cagttcgcgg gccccttccc ggtctctgac cacgcgcacc accgagtcgt cgcacggcac
     3001 gtggatcgga cgattgtggt cgaggaccgc gtcgcacagc accgaaagcc gtttgcgggc
     3061 atcgtcgtcg gtgaagcaga tgggctcgtc ggagcggttc gcgctggtca gcaccagcgc
     3121 gtcgggcacc cgctccgcgg cgccgggcac gggcgccagc agcaggtgat gaatggggga
     3181 gtagggcagc atcagcccga gcagtgggct gccgggcgcg acggcgtcgg cgaccggcgc
     3241 gccccggcgg cgcctcagca acacgatcgg ccgggcggga ctcgacaaca ccacagcctc
     3301 ggcggcgtcg acgtgcgcgt agcggcgcgc gacatcgagg tcgcggacca gcatcgcgaa
     3361 aggcttcgcg ccccgcgcct ttcgtgatcg cagttcacgg accgccccgt tgtggtccac
     3421 agcgcaggcc aggtggtaac cgccgatgcc cttgatggcg accaccacac cggccgccag
     3481 ggcgccttga gtggccgcca gggccgcatc cgatgcctgt gtccgttctc ccccttgggt
     3541 tgcgcccgct ggacggaac
//
LOCUS       DASSGQ010000143         3694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_557124,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000143 DASSGQ010000000
VERSION     DASSGQ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557124"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /gene="eccB"
                     /locus_tag="VFI55_06520"
     CDS             complement(<1..961)
                     /gene="eccB"
                     /locus_tag="VFI55_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccB"
                     /protein_id="HET7073719.1"
                     /translation="MGFDNFPGGILDRLQPWQPTTRLQVSGYRFLLRRIECALLGRDI
                     GVADKPQGARMTSLTVGCVVAAITLAGCAVLALLRPHVGFDQAPIVMGQRSGALYVRV
                     GDVWHPVLNLASARLVAATAADPVPVRESDLGRAKHGPLLGIPGAPQFLGQPLSAGES
                     AWAICDADGSAATTVIAGPTEGSSIRRVGRGQAILVAPAPGSPAYLLYDGQRAMVDLS
                     DVAVLRALRLEGRAPRPISPSLLNAVPEAPPIGVPRIRGAGGAAAGLPGFPVGSVLRV
                     TRADSDEYYVVLPGGVQRIGQVAADLLRSSDSQGTAHAITVAPD"
     gene            1145..2083
                     /locus_tag="VFI55_06525"
     CDS             1145..2083
                     /locus_tag="VFI55_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HET7073720.1"
                     /translation="MGASALVTATGLLVTPVAASIASADNCPDAEVVFARGTNEPSGL
                     GRVGDAFVDSLRQQTGGLNIATYGVNYAASKLQLHGGDGANDVITHVKQTVSSCPNTK
                     IVLGGYSQGASVIDIVAGVPIGGINWGSSLPPELANNVAAVVTFGDVADRAGGSLPTQ
                     STMFGSRSIDLCNPNDPICHAGPGNEWSGHTEGYVPVYTSQAAAFVASRLLAGTGQQV
                     PGYGPSIPAYGPQSPGFGPQNPGYGPQNPGYGPQNPGYGPQNPGYGPQNPGYGPQNPG
                     YGPQNPGYGPQTPGSGPSIHGDVPAPDTPSPQAPLV"
     gene            2123..2491
                     /locus_tag="VFI55_06530"
     CDS             2123..2491
                     /locus_tag="VFI55_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7073721.1"
                     /translation="MSLDPVCFSDQWVRAWNAHDVEAVLAHFHDDVVFTSPVAASFLP
                     ETAGRIRGKPALRHYWTQALQRIPNLQFVVEGVYRGIDTIVIAYRNQDDRRVSEVLRF
                     NDDLVVEGHGTYIVSGQVSD"
     gene            2651..3259
                     /locus_tag="VFI55_06535"
     CDS             2651..3259
                     /locus_tag="VFI55_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003411863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HET7073722.1"
                     /translation="MPFPVPFAMPFSKQLPVASAAACPGTEVVFARGREEPPGVGYVG
                     DAFVNALRAKVHAPVGSYGVNYPADISPAEGANDMSGHVQSMARSCPNTREVLGGYSL
                     GAAAADLVVAMTQPAFGFTDPLPPRLDQHVAAVALFGNGTRRVLGPVPDFSPAFAGKT
                     IDQCASGDPICSNGTQWSSHLQPSYINSGLVDQAASFVAGKL"
     gene            complement(3285..>3694)
                     /locus_tag="VFI55_06540"
     CDS             complement(3285..>3694)
                     /locus_tag="VFI55_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET7073723.1"
                     /translation="TAWPRPLDVEAMAVASRNLVGLHDFAAFCRQRESATTIRDLQRL
                     DWSRDGDLMTAHVTADAFCWSMVRSLVGALLAVGEGRRPQPWCGELLSATDRSSDFAA
                     APAHGLTLIGVDYPPDDQLAARNVITRDLRTRD"
BASE COUNT          648 a         1316 c         1157 g          573 t
ORIGIN      
        1 catcaggcgc cacagtaatg gcgtgcgcgg tgccctgcga gtcgctggaa cgcaggagat
       61 cggctgccac ctgaccgatg cgctggactc cgccgggcaa aacgacgtaa tactcgtcac
      121 tatcggcccg tgtgacgcgc agcacactgc cgacgggaaa accgggcagc ccggccgccg
      181 cgccgccggc gccgcgaatc cgtggaacgc cgatcggcgg cgcctcgggc acggcgttca
      241 gcaacgaggg cgaaataggc cgtggcgcac ggccctccag cctcagcgca cgcagaaccg
      301 cgacgtcgga gagatccacc atggcccgct gtccgtcgta gagcagatac gccggcgaac
      361 ccggggcggg cgcgaccaaa atggcttgcc cgcggcccac ccgacgtatc gacgatccct
      421 cggtgggtcc cgcgatgacc gtcgtcgccg cggacccgtc cgcatcgcag atcgcccagg
      481 cggattcacc cgcggacagt ggctggccga gaaattgcgg cgcacccgga atgccaagca
      541 gcgggccatg tttggcacgc cctaggtcag actcgcggac cggcaccgga tcggcggccg
      601 tggccgcgac caaccgcgcc gaagccaggt tcagcaccgg atgccaaaca tcgcccaccc
      661 gaacgtagag ggctccggat cgctgaccca tcacgatcgg tgcctgatcg aacccgacat
      721 gcggccgcag caaagccagc accgcacacc ccgcgagggt gatagccgcc actacgcacc
      781 ccaccgtcag cgatgtcatt cgcgcaccct gcggtttgtc ggccacgccg atatcccggc
      841 ccagcaacgc gcactcgatg cgacgcagca ggaatcggta accgctgact tgcaggcgcg
      901 tcgtcggttg ccagggctgc aaacggtcca aaatcccccc gggaaaattg tcgaatccca
      961 aacttcgcgc ccacgttatg cgctgtttga ccgcccgcgt ggagggttat ccacaggtga
     1021 tcggagtttc gtcacgcctg aatccgggta aaatttgggc gtgaaaactt accgcatacg
     1081 tttccctcgg atccctcgga tccctcgggc gggcagcgcg cggtcagcgg gtcgctgggt
     1141 cggcctgggg gcttctgcac tcgtcaccgc cacgggcctg ctcgtcacgc ctgtcgcggc
     1201 atcgatcgcc tccgccgaca actgcccgga cgccgaggtc gtgttcgcgc gcggcaccaa
     1261 cgagccctcg ggactcggcc gtgtcggcga cgccttcgtc gattcgttgc gccagcagac
     1321 cggcggcctg aacatcgcca catacggcgt caactacgcc gccagcaagc tccagctgca
     1381 cggcggtgac ggcgccaacg acgtcattac ccacgtcaaa caaacggtgt cctcctgccc
     1441 gaacaccaag atcgtgctgg gcggctactc gcagggcgcc agcgtgatag acatcgtcgc
     1501 cggcgttccc atcggcggca tcaactgggg cagttcgttg ccgccggagc ttgccaacaa
     1561 cgtcgcggct gtcgtgacgt tcggcgacgt ggccgatcgc gcgggcggat cgctgcccac
     1621 tcagagcacc atgttcggct cgcggtcgat cgatctgtgc aaccccaacg acccgatctg
     1681 ccacgcgggt cccggcaacg agtggagcgg tcacaccgag ggctacgtcc ccgtctacac
     1741 cagccaggcg gcggcgttcg tcgcgtccag gctgttggcc ggaaccggcc agcaggtgcc
     1801 agggtatgga ccgtcgatcc ccgcgtatgg cccgcagtcg cctgggttcg gcccgcagaa
     1861 ccccggttac ggcccacaga accctggcta cggcccgcaa aaccccggct acggcccgca
     1921 aaaccccggc tacggcccgc agaaccccgg ctacggcccg caaaaccccg gctacggccc
     1981 gcagaacccc ggttatggtc cgcagacccc ggggtctggc ccgtcgatac acggcgacgt
     2041 ccccgcgccg gacaccccgt caccccaggc cccgctggtc taagccgctg ggccgccggc
     2101 cctgccgact acagtgccca ccgtgagcct tgatccggtc tgcttcagcg accaatgggt
     2161 acgcgcatgg aacgcccacg atgtggaggc ggttcttgcg cactttcacg acgacgttgt
     2221 gttcacgtcg cccgtggctg catccttcct gccggaaacg gcaggaagga tccgcgggaa
     2281 gcccgcactg cggcactact ggacgcaggc actgcagcgc attcccaacc ttcagttcgt
     2341 cgttgagggc gtctatcggg gaatcgatac catcgtcatc gcataccgca accaagacga
     2401 ccgccgggtc agcgaagttc tccggttcaa cgacgacctc gtcgtcgaag gccatggaac
     2461 gtacatcgtc tcaggccagg tctcggacta ggtctctgga gcaagcgctt actatcgggc
     2521 catgaggcgc tacccggctg acccaccgcc acaggcgaat tcggccgccg ggccccgtgt
     2581 actacaccgc ttgcacggtt gggcggctgc cgtgtttctt gccgcaacgt cggtgatcgc
     2641 ccctgcagcc atgccctttc cggttccctt tgcgatgcct ttttcgaagc agcttccggt
     2701 ggcgtcggcg gcggcctgcc cggggaccga agtggttttc gcgcgaggca gagaggaacc
     2761 acccggcgtt ggctacgtgg gcgacgcgtt cgtcaatgcg ttgcgcgcca aggttcatgc
     2821 gccggtcggg tcctatggcg tcaactatcc ggctgacatc agccccgccg agggcgccaa
     2881 cgacatgagc gggcacgtcc aatccatggc gagaagttgc ccgaacaccc gcgaggtgct
     2941 cggcggctac tccctgggcg ccgcggcggc tgacctcgtg gtcgcgatga cccaaccggc
     3001 attcggattc accgacccgc tgccgccgag gttggatcag cacgtcgcag cggtggcgtt
     3061 gttcggtaac ggcactcgac gagtccttgg cccggtcccc gatttcagcc ccgcctttgc
     3121 gggcaagacg atcgatcaat gcgccagcgg cgaccccatc tgttcgaacg gcacacagtg
     3181 gtcgtcgcat ctacagccgt catacatcaa ttccggactg gtggatcagg ccgcaagctt
     3241 cgtcgccggc aagctgtgac cgcgagctcg ccatgcgcgg cagctcagtc gcgcgtccgc
     3301 agatcccgcg tgatgacatt ccgcgccgca agctgatcat cgggtgggta atcgacgccg
     3361 atcagggtca acccgtgcgc cggagccgcg gcgaagtcgc tggaacggtc tgtcgcggaa
     3421 agcaattcac cgcaccaggg ctgcgggcgg cggccttcgc cgacggccag cagcgccccg
     3481 accaacgacc gcaccatcga ccaacagaac gcgtcggcgg tcacgtgcgc ggtcatcaag
     3541 tcaccgtcgc gcgaccagtc cagccgctgc agatcgcgga tagtggtggc gctctcgcgc
     3601 tgacggcaga acgcggcgaa gtcgtgcagc cccaccaagt ttcgcgacgc caccgccatc
     3661 gcctcgacgt cgagtggtct cggccaggcc gtta
//
LOCUS       DASSGQ010000144         4422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1195602,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000144 DASSGQ010000000
VERSION     DASSGQ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195602"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..782)
                     /locus_tag="VFI55_06545"
     CDS             complement(204..782)
                     /locus_tag="VFI55_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7073724.1"
                     /translation="MGGTALAVPTDLADAAAIRGAVARVATELGPIDVLINAAGTDVP
                     GAVETLDPASWDRVIAVNLTAPFLLAKAVWPHLRRAGGGTIINVSSVAGRRGWANAAA
                     YRATKFALTGFTQSLAAEGRQHGIRACVIYPGAMATAWGVWDATQRHSAGVSERPENE
                     ALPASQVAKPDLVDRRGTCQPRAQRGHDQPAA"
     gene            complement(742..1059)
                     /locus_tag="VFI55_06550"
     CDS             complement(742..1059)
                     /locus_tag="VFI55_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7073725.1"
                     /translation="MGLIGRYDWWDVLPPLEGSHTASDAAGALSNVPGVVDSLSGGQL
                     SDRVAVVTGASSGLGRATAARLATDGAAVALLARSAEDLAITQAGITESGRHRAGGAD
                     RLG"
     gene            2422..3564
                     /locus_tag="VFI55_06555"
     CDS             2422..3564
                     /locus_tag="VFI55_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7073726.1"
                     /translation="MTVTDPTTTTAGALIVLGLTAQSNGVPGAAAKLVGLYIRLQAAM
                     LPDAGAGVTALQTQPTSAPAFVASEQDVFLANRGKSSPVVSAVYPSGATPMLDFPMVH
                     LARPTADPLMLAAAAQFEDQLTTSAARGHLADAGLRGPSGTPLTADHARTGVSAQRVT
                     PAPTTITAAQQATALRLWRAAARPSQLLAVIDVSGSMGDPSGIGLSKIQVVSAAARTA
                     MTAIPDDWTLGLWTFSTHPPPDTDWTERIPLGLVKTQRAALTAAAGGLPSLVGGNTGL
                     YDTTLAAFHDISAHYGPASVNVVALLTDGQNVDPGDLDLPTLLSQLKAEYQPGKPVHI
                     VTIGFGNDADANALKQISDATHGQSYLVTDPKDILGVILDSIIANN"
     gene            3661..3960
                     /locus_tag="VFI55_06560"
     CDS             3661..3960
                     /locus_tag="VFI55_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073727.1"
                     /translation="MTTTGFDLTAYKTAQQSDWTTTAQGWQQFEPALQRLDQRKNLVT
                     QGAFGQIGEHLRATFPHDQCLKHGSRRYAGQVGGHRRWSIVSSPTGRWGAATSDD"
     gene            complement(3961..>4422)
                     /locus_tag="VFI55_06565"
     CDS             complement(3961..>4422)
                     /locus_tag="VFI55_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073728.1"
                     /translation="PGQLAPAVLSAVTGTVLSGLSGHGLVPVRGLSLRGINKGLAVLA
                     VAAALSSVAYAATMIQAARAGHSDDDTWGLMHLPTQAAFALSVARVAVLAVFGRCGQV
                     GMVAGVSLVCGGIRAVVGPAQCRLPRALGQRGKNWWGRCDRLGVRHSRSAP"
BASE COUNT          756 a         1570 c         1417 g          679 t
ORIGIN      
        1 ggccgcgtat tcgctgtagt ccccgcccgg aagctgatgg ggcgcgtcgc ccgctatccc
       61 gtcagttatc ccacgagcgg tgtctgcagt gtaggttgcg gccgctcagc aggtcgtcgc
      121 tccacacctc gacccactcg cgctgtgcgt ccgaccgggc ctggtggacg ggtgcagcgt
      181 gttctcgcta gggccagccc tgctcaagca gcgggctgat cgtggcctcg ttgagcacga
      241 ggttggcagg tgccgcggcg atccacgaga tcaggttttg cgacctggct ggccggtagc
      301 gcctcgttct cgggccgctc agacacaccc gcggagtgac gctgggtggc gtcccacacg
      361 ccccaagcgg tcgccatcgc gccgggatag atcacgcatg cccgtatccc atgctgtcgt
      421 ccctcggcgg ccaggctctg ggtaaagccg gtgagcgcaa actttgtcgc acggtacgcc
      481 gcggcgttgg cccagccgcg gcgtccggcg accgacgaga cgttgatgat cgtgccgccg
      541 ccagcgcggc gtaggtgcgg ccacaccgcc ttggcgagca ggaacggcgc agtcaagttg
      601 accgcgatca cccgatccca gctagccggg tcgagggtct cgaccgcgcc gggcacgtcg
      661 gtgccggccg cgttgatcag tacatcgatg ggccccaact cggtggcgac gcgcgccaca
      721 gcaccccgga tcgccgctgc gtcagccaag tcggtcggca ccgccagcgc ggtgccgccc
      781 actttcggtg atcccggctt gcgtgatggc caagtcttcg gcgctgcgcg caagcaacgc
      841 caccgccgcg ccatctgtgg cgagccgagc ggcggtggcc cggccaagac cgctgctggc
      901 accagtcacg accgccaccc ggtccgacag ctgcccaccc gacaaggaat ccaccacccc
      961 cggcacgtta cttagcgcgc cagccgcatc ggaagcggtg tgggaaccct ccagcggcgg
     1021 tagcacatcc caccagtcgt agcgaccgat caggcccaca gccggacgtg tccgacaagc
     1081 tgtgaagcgc cgcaagcacg cgtcgtcggc ccgctgccgt ggcgcggtac accttaggtt
     1141 gcgtccgtac ggccgaccac gctgagccgg cgttacaccg accggtcggc cgtcccgcga
     1201 attagaccgt gattgcgatg cggcccctgt tgtgtgtgcg ctctcctggt agcggaccgc
     1261 ttagccgcct cgctaagtgg ataggtccga tcgaggatcg gggtgatctg tccgccttca
     1321 cgaaggggat ttagttcaag gaggtaccga gcgtgttccc ggaccacgta tgcgccgagc
     1381 ttctgtcgca cgaagggcga tagcaggctt gcccacaatt gccgttgaat gccgccgatc
     1441 caccggtcac cgtcaccgcc gacgatgacc aaacgcccgc ggacagccag agcgcgccgc
     1501 agccgcgata cccggcttcg tccgaagacg tcaacgatga ggtcgtaccg cagcctgccg
     1561 tccgcgaagt cctcccgggc gtagtcgacc agacaggatc atcccggaca gcaagttgcc
     1621 ggatcctgcg gaactaagtg gctcggtggg caagctgctg ggccagccgc ttttaccgca
     1681 agtagttacg cggctgcttg ctgcacatac gtcgacgcga cggtgggaaa ccctcgacgg
     1741 cggcgccgtg cgtgggtaga accattccct ggcgacgttt tttcgggagt gtccagccga
     1801 acccggcagg tcttagggac gggtccagcc gaaccaacag gcgaggaggt ggccctgcca
     1861 atggcgggta agcacatcgc cgcacgccgt gcagatgatc cgctcccgca ccggacccga
     1921 cgacgcgccg gccggttcgt cgtgatggtc ttggtggtgt ttctgctggc tgcggctggc
     1981 acggcgaccg ttctcgcgct gaggggccgt acccaccacc catcagttgc cgctgcgtcg
     2041 ccgagctgca ccggcgaggt ttccgtgccg gtgatcgtgg ctccgtccgt cgcagcagct
     2101 gtaggcgcaa tcgccaacca atggaccgga tctcacccgg ccgtacaggg ccgctgcatc
     2161 cgcgtcgtgg tcactagtca ggatcctgcc gtggcgacga agtcgattgc ctccggcgcc
     2221 gctccggcag tgtggattcc agactcgaca gtgtggagcg ggaggttggc cgtagcggcg
     2281 ccagcgctcg cgtcagcggt caaggtggat ggctctatcg cgagctcccc gctcgttgtc
     2341 gcagcggcgc cgagcaaggc ggcaccgatc gccgcagcgg ctaaagcagg ctgggccggc
     2401 gcgctcgctg gcaccacgat ggtgacggtc actgacccga cgacgaccac ggccggcgcc
     2461 ctcatcgtgc tcggcctcac ggcacagtcc aacggcgtgc ctggcgccgc ggcaaagctg
     2521 gtcggcctct acatccgcct acaggcggcg atgctgcccg acgccggggc tggggtcacc
     2581 gcattgcaaa cgcagcccac gtctgcgccg gcattcgttg ccagcgaaca agacgtgttc
     2641 ctcgccaatc gcggaaaatc gtcgccggtt gtctcggccg tgtatcccag cggcgctacc
     2701 ccgatgctcg acttcccgat ggtgcacctg gcccgcccca cggccgaccc gctcatgctc
     2761 gccgcggcgg cgcaattcga ggatcagctg accacctccg cagcgcgcgg ccacctcgcg
     2821 gacgctggcc tacggggtcc gtcaggcacg ccgctaactg cagatcacgc gaggaccggc
     2881 gtatccgctc agcgggtgac accggcgccg acgaccatca ccgcggcgca gcaggcgacg
     2941 gccctacgac tgtggcgggc agccgccagg ccgtcgcagc tgctcgcggt catcgatgtg
     3001 tccggttcca tgggcgaccc gtcgggaatc gggctgtcca agatccaggt ggttagcgcc
     3061 gccgcgcgaa ccgcgatgac cgcgatacct gacgactgga cactcgggct gtggacgttc
     3121 tcgacccatc cgccgcctga cacggactgg accgaacgca tcccgctcgg gctggtgaag
     3181 acgcaacgag ccgcgcttac cgccgcggcc ggaggactgc cctcgctcgt cggcggtaat
     3241 acgggtctgt acgacacgac gcttgccgcg ttccacgaca tctcagcgca ttatggcccg
     3301 gccagtgtca atgtggtcgc tctgttgacc gacggccaga acgtcgaccc cggcgacctt
     3361 gatctgccca cgctgctgag ccagctcaaa gccgagtacc agccggggaa gcccgttcat
     3421 atcgtgacga ttggcttcgg caacgatgcg gacgcaaacg cgttgaaaca gatctccgac
     3481 gcgacgcatg gccagagcta cctggtcacg gatcccaagg acatccttgg cgtcatcctc
     3541 gactcgatca tcgccaacaa ctgacgtccc tgagcaattt cggccggcgg cctaccacca
     3601 agacgacacg accgatgcgc acgctgaatc acccatccag caaccggcaa agaggctgcg
     3661 atgaccacga ccgggttcga cctgaccgcc tacaagaccg cccagcagag tgactggacc
     3721 accaccgccc agggctggca gcagtttgaa ccggccctcc agcgcctcga tcaacgcaag
     3781 aatcttgtga cgcagggtgc gtttggccag atcggcgagc accttcgggc cacgttcccc
     3841 catgaccagt gcctcaagca tggctcgcgc cgatacgccg gtcaggtcgg tggccaccgc
     3901 cgatggtcga ttgtcagctc accgacggga cgatggggtg ccgccacgtc cgatgattga
     3961 tcacggtgcc gaccgcgagt gccgcacccc cagacgatcg cagcgacccc accaattctt
     4021 cccacgctgc ccaagtgccc ggggtaggcg acactgagca ggcccaacca cagcgcggat
     4081 accgccgcag accaggctga cacctgccac catgccgact tggccgcacc ggccaaacac
     4141 cgcgagcacg gcgacacggg caaccgacag ggcgaacgcg gcctgcgtcg gcaggtgcat
     4201 caacccccac gtgtcatcgt cagagtgccc ggcccgcgcc gcctggatca tcgtcgcggc
     4261 gtaggccacc gagctcagcg cagcagccac cgcgaggacc gccaagcctt tgttgatgcc
     4321 ccgcagggac aacccgcgaa ccggcaccag cccgtggcct gacaggccag acagcaccgt
     4381 gccggtgacc gccgacagca cggccggcgc gagctgccct gg
//
LOCUS       DASSGQ010000145        10031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_922293,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000145 DASSGQ010000000
VERSION     DASSGQ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10031
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922293"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..991
                     /locus_tag="VFI55_06570"
     CDS             104..991
                     /locus_tag="VFI55_06570"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7073729.1"
                     /translation="MSEPRTIVITGASRGLGLASAAHMYQRGWRVVAAMRSPESGLSA
                     LRAKTGAAEDDPRLVAIPLDLTDPASIAAAAKAIEAAAGAPHALVHNAGISAAGALEE
                     TPMDLWERMFATAVFGPVRLTKALLPSMRAAGRGRIVMISSQGGVRGMPEIAAYSAAK
                     GAVERWAESLAGEVAPFGLGVSVLVTGTFDTDIITDAGTSDHRDFAGPYAPLHRKIDR
                     RGRAAMRLARPPDRFARGLVKAVEDRAPFARHAVGVDARMLMVANRLLSGKALHRVTR
                     LAMGIPHSGALSSASSKES"
     gene            998..2482
                     /locus_tag="VFI55_06575"
     CDS             998..2482
                     /locus_tag="VFI55_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7073730.1"
                     /translation="MASTVKVRFEPKMMIDGELVDGRAGTFANVNPATEETLGEVADA
                     STEDMHRAIDAARRAFDETDWSTNRALRQRCLLQLHEAIDAEREELREELILEVGAPR
                     AITFGPQLDAPLEDGLRYPARLIDEYPWETDLGDKTISLTGTLTTRKVWREPVGVVGA
                     IVPWNFPFEITINKIGQALGTGNTMVLKPAPDTPFNATRLGRLIAENTDIPPGVVNVV
                     TASDHLVGEELTLSPKVDLISFTGSTAVGQRIMEKGALTMKRLFLELGGKSATIVLED
                     ADFPMACVIGIAPCMHAGQGCANPTRMLLPRSRYDEGVAILKSIYDNVTCGDPQDPGT
                     LCGPVISDKQRKRVQHYINKGVEEGATALVGGPDAPTGFDRGFFVRPTLFTNVDNSMT
                     IAQEEIFGPVLSVIPFDDEEEAIRIANDSRYGLAGNVMSGSLEHSLAVARRIRAGFMG
                     VNGGAPYGADTPFGGYKYSGVGRQNGVAGFDQYTEIKSVGYPAG"
     gene            2505..3719
                     /locus_tag="VFI55_06580"
     CDS             2505..3719
                     /locus_tag="VFI55_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7073731.1"
                     /translation="MQLFDDLEDFGAFDDVVSGDVRDPYTELARLRREEPVQRIDFSG
                     MPHEESKPVFIVYRYEEVQQMLRDNETFSSAIIIDAFGDVLGRHVMLGMDEPEHGRHR
                     ALVAKAFSQKALARWEDELVGKVGNELIDQFAARGRADLVKEFTFPYPTQIIAGLLGL
                     PREDYPQFQRWSISLLSFTINPERGRAASAALEEYFAPILAARRAEPRDDLISGLAQA
                     EIDGEKLSDVEIFSFLRLLLPAGVETTYRSLGNMLFGLLSNTGQLEAVRADRSLLPQA
                     IEEAVRWEPPLLTITRVATRDTELAGVAIPAGSAVMPMLGAANRQDDRYPEPDRFDIF
                     RSPRAHIGFGHGVHVCLGMHLARLEMRVALNLLFDRLANLRFDPGADDPHIRGQVFRS
                     PTSLPVAFDPCA"
     gene            3810..5078
                     /locus_tag="VFI55_06585"
     CDS             3810..5078
                     /locus_tag="VFI55_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7073732.1"
                     /translation="MSDFESVDFFTDASLVPDPYPYFDQLRERCPVMSRPGQGVVAIT
                     GHQEALAVYKDPAFSSCVAVAGPFSGLPFEPHGDDIGALIEQHRSQIPMSEHIVTQDP
                     PEHTRTRGLLSRLLTPKRLKENEEFMWRLADAQLDEFVSRRTCEFLNDYARPFTGLVI
                     ADLLGVPPEDHDEFRAVFFGKVSGGIEDDSITRAHNPLEYLDDKFTTYITERRREPRS
                     DVLTELALAKYPDGSTPEVIDVVRLATFVFAAGQETTTKLLSFAVRMIAENPQIQTLL
                     REDRSKIPTFVEETLRTESPVKCHFRMARTATSIGDTKIAAGSTVMLLPGASNRDARK
                     FEEPNEFRIDRANVREHVAFGRGNHSCPGAPLARAEGRISLNRILDRMSGIGILETEH
                     GSPDARRYTYEPTWQMRGLTELHLAFSPTG"
     gene            5196..6344
                     /locus_tag="VFI55_06590"
     CDS             5196..6344
                     /locus_tag="VFI55_06590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET7073733.1"
                     /translation="MAEAVIVEAIRSPIGKRNGGLSGVHPADLSAQVLNGLADKAGID
                     PGIVEDVIWGCVMQAGEQALDIARTAVLAAGWPETVPGVTVDRQCGSSQQSIHFAAAG
                     VVAGHYDVVVAGGVESMSRIPMGASLAGGGRPYPETFMTRYEGRVPNQGLGAEMIAEQ
                     WGLDRLALDEFALASHEKAAAAQDSGAFDDQIVGIKDQDSNVVLKDEGIRRGTPMEKM
                     AALKPAFKEDGVIHAGNSSQISDGSAALLFMSAEKAKSLGLKPIAKVHTATLAGADPV
                     IMLTAPIPATQKVLKKSGLSIDDIGAYEVNEAFAPVPLAWLKDIGADEKKLNPNGGAI
                     ALGHPLGGSGARIMTTLLYHMRDKGIRYGLQTMCEGGGQANATIVELL"
     gene            6341..7141
                     /locus_tag="VFI55_06595"
     CDS             6341..7141
                     /locus_tag="VFI55_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HET7073734.1"
                     /translation="MTDPAAAPAALVDRSGNVMVITINRPDARNAVNAAVSIGVGDAL
                     EEAQRDPDVRAVVITGAGDKSFCAGADLKAVARRENLYHPDHPEWGFAGYVHHFVDKP
                     TIAAVNGTAFGGGTELALASDLVVASERAQFGLPEVKRGLIAAAGGVFRIVSQLPRKV
                     AMELLLTGEPLAASDALEWGLVNQVVKDGSVLDAALALAARVTVNAPLSVQASKRIAY
                     GVDDGVIAAEEVGWDRTLREMRSLIRSEDAKEGPLAFAEKRQPVWKAR"
     gene            complement(7159..8175)
                     /locus_tag="VFI55_06600"
     CDS             complement(7159..8175)
                     /locus_tag="VFI55_06600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7073735.1"
                     /translation="MTDYDTDLLVVGGGPGGLATALHARRLGLSVIVADPRESPIDKA
                     CGEGLMPGGLAELTSLGVDPAGMPFRGIAYLSEHRRAEARFRNGPGRGVRRTTLHAAL
                     AARAKEQDAEWIQERVTTVEQDAHGVTAAGVRAKWLVAADGLHSTVRRAVGIQTTAGT
                     PRRYGVRWHYKVPVWSEFVEVHWSRWGEAYVTPVEPDLVGVAILSRGQPDLAWFPSLA
                     QHLRGASRGHARGCGPMRQVVSRRVAGRVLLVGDAAGYEDALTGEGISLAVKQAAAAV
                     NAIVNDEPSSYEGAWHRVTRDYRMLTRGLVLASTPRVTRRALVPACTALPAVFRTAVN
                     RLAV"
     gene            complement(8172..8672)
                     /locus_tag="VFI55_06605"
     CDS             complement(8172..8672)
                     /locus_tag="VFI55_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HET7073736.1"
                     /translation="MYYLLILAVAVERLAELIVAKRNARWAFAHGGKEFGQDHYPVMV
                     ALHTALLLGCLIEPWALHRPFIGWLGWPMLAVAALAQVLRWWCITTLGPRWNTRVIVL
                     PEAPLIKRGPYRWLHHPNYVAVVAEGIALPLVHTAWLTALIFTLANAMLLRVRLRVEN
                     SALGYA"
     gene            complement(8687..9781)
                     /locus_tag="VFI55_06610"
     CDS             complement(8687..9781)
                     /locus_tag="VFI55_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HET7073737.1"
                     /translation="MDTITGDRFSRVGLKRPTANADPSVAAVAVKFPPNLYSQEEVIG
                     ALTDFAGPEFRRFAMSSGVTTRHTALPLTRYKELTGFTEANDNFVEVALDLAEQALLT
                     ALDQANLDPSEVDVVFSTTVTGLAVPSLEARLATRVGLRQDVKRVPLFGLGCVAGAAG
                     LARVHDYLRAFPDHVAVLLAVELCSLTIQREDVSIANLVASSLFGDGAAVVIATGADR
                     TPVGPKMLATRSMTYPDTEDALGWNIGGDGFQIVLSAEISTVVEKYVGDDIRKFLAHH
                     GLTTSDVSTWLLHAAGPKVIDAVEDALELPADALERTRTSLRDNGNLSSVSVLDILHA
                     TMADPPSPGTIGLMIAMGPGFSAEFVLLSW"
BASE COUNT         1767 a         3299 c         3338 g         1627 t
ORIGIN      
        1 gcccgtactc ggtcggcatc tggtcgacct ggatgagtgc ccaacccaac gggcgcccga
       61 acgtcgaact gcctgccgac ggcggaaggt gcgcgccgcg cacatgagcg agccccgcac
      121 catcgtcatc actggcgcgt cccgcgggct tggcctcgcg tccgcggcac acatgtacca
      181 gcggggctgg cgtgtcgtgg cggccatgcg ctcgcccgag tccggccttt cggcgctgcg
      241 cgcgaagacc ggcgccgcag aagacgatcc gcgattggtc gccatcccgt tggatctcac
      301 cgacccggcg tcgatcgcgg ccgcggccaa ggcgatcgag gcggccgccg gggccccgca
      361 cgcgctggtg cacaacgccg ggatctccgc tgcgggagcg ctggaagaga caccgatgga
      421 cttgtgggag cgcatgttcg cgaccgcggt gttcggaccg gtgcggctga ccaaggcgct
      481 gttgccgtcg atgcgggccg cgggacgggg gcgaatcgtg atgatctcga gtcagggcgg
      541 cgtacgcgga atgccggaga tcgcggccta ctcggccgcg aagggcgcgg tggaacgctg
      601 ggcggagtcg ttggccgggg aggtggcgcc cttcggcctc ggcgtcagcg tattggtcac
      661 cgggaccttc gacaccgaca tcatcaccga cgcggggacc tccgatcacc gcgacttcgc
      721 gggaccgtac gccccgttgc atcgcaagat cgaccggcgg ggtcgcgcgg ccatgcgcct
      781 ggcgcggcca ccggaccgct tcgcccgtgg gcttgtcaag gctgtcgagg accgtgcgcc
      841 gtttgcccgg catgcggtcg gcgtcgacgc ccgcatgttg atggttgcca accggctgct
      901 ttccggtaag gctctccata gggtgacccg tctggcgatg ggtatcccac actctggcgc
      961 tctgagcagc gcgtcgtcga aggagagctg aacaaacatg gctagtactg taaaggtccg
     1021 cttcgagccg aagatgatga tcgacggcga gctcgtcgac ggacgagcgg gcaccttcgc
     1081 caacgtcaac ccggcgaccg aggagaccct cggggaggtc gccgacgcgt caaccgaaga
     1141 catgcaccgg gccatcgacg ccgcccgtcg ggccttcgac gagaccgatt ggtcgaccaa
     1201 ccgtgcgctg cggcaacgct gcctgttgca gctgcacgag gcgatcgacg ccgagcgtga
     1261 ggagctgcgc gaggaactca tcctcgaagt cggtgccccc cgggcaatca ctttcggtcc
     1321 ccagctggat gcaccgctgg aggacgggct gcgctatccg gccaggctca tcgacgagta
     1381 cccgtgggaa accgacctgg gcgacaagac gatcagcctc accggcaccc tcaccacccg
     1441 caaggtttgg cgggaaccgg tgggggtggt cggcgcgatc gtgccgtgga acttcccgtt
     1501 cgagatcacc atcaacaaga tcggacaggc gctgggcacc ggcaacacca tggtgctcaa
     1561 accggcgccc gacacgccgt tcaatgcgac ccggctcggc cggctgatcg ccgaaaacac
     1621 cgacatccca ccgggtgtcg tcaacgtcgt gaccgcttcg gatcacctgg tgggcgagga
     1681 gctcacgctg tccccgaagg tcgacctgat ctcgttcacc ggctccaccg ccgtcggcca
     1741 gcggatcatg gaaaagggtg cgctgacaat gaagcggctg ttcctcgagc tcggcggcaa
     1801 gtcggccacc atcgtgctgg aggacgcgga ttttccgatg gcgtgcgtga tcggcatcgc
     1861 gccgtgcatg cacgccgggc aagggtgtgc caacccgacc cggatgctgt tgccgcggtc
     1921 tcgctacgac gagggtgtgg cgatcctgaa gagcatctac gacaacgtca cgtgcggcga
     1981 cccgcaggat cccgggacgc tgtgcggccc ggtgatttcg gacaagcagc gcaagcgcgt
     2041 gcagcactac atcaacaagg gcgtcgaaga gggcgccacc gcgctggtcg gcggacccga
     2101 tgcgcccacc ggcttcgaca ggggattctt cgtcaggcca acgcttttca ccaacgtcga
     2161 caactccatg acgatcgcgc aggaggagat cttcggtccg gtgctctccg tcatcccgtt
     2221 cgacgacgag gaggaggcga tccggattgc caacgacagc aggtacggat tggcgggcaa
     2281 cgtgatgtcg ggctcgctgg agcactcgct ggcggtggcc cgccggatcc gggccggctt
     2341 catgggtgtc aacggcggcg ccccgtacgg cgccgacacg ccgttcggcg gatacaaata
     2401 cagcggcgtg ggccggcaga acggcgtggc cgggttcgac cagtacaccg agatcaagtc
     2461 cgtggggtat ccggccggct agcggcgcaa cggaaggggc atgaatgcag ctgtttgacg
     2521 acctcgagga tttcggggcc tttgacgacg ttgtctcagg tgatgttcgg gacccctata
     2581 ccgagttggc ccgtctgcgg cgcgaggagc cggtgcaacg catcgacttc tcgggcatgc
     2641 cgcacgagga gtccaagccg gtcttcatcg tgtaccgcta cgaagaggtg cagcagatgc
     2701 tgcgggacaa cgagacgttc tcctcggcga tcatcatcga cgcattcggg gacgtgctgg
     2761 gcaggcacgt catgctcggg atggacgagc cggaacacgg tcgtcaccgt gctctggtgg
     2821 caaaggcgtt ctcgcagaag gcattagcgc gatgggagga cgaactggtc ggcaaggtcg
     2881 gcaatgagct gatcgaccag ttcgccgccc gcggccgcgc cgatctagtc aaagagttca
     2941 cttttcccta tccgacgcag atcatcgccg gcctgctggg cttgccgcgg gaggactatc
     3001 cgcagttcca gcgatggtcg atctcgttgc tgagcttcac catcaacccg gagcgcggcc
     3061 gcgcggcctc cgcggcgctc gaggagtact tcgcgccgat cctcgcagcg cggcgcgcgg
     3121 agccgcgtga cgatctgatc agcggtctgg cgcaggcgga gatcgacgga gagaagctgt
     3181 cggatgtgga gatcttctcg ttcctgcggt tgctgcttcc cgccggcgtc gaaaccacct
     3241 accgctcgct gggaaacatg ctgttcggat tgctgtccaa cacaggacaa ctcgaagcgg
     3301 tacgggccga tcgttcgctg ctgccgcagg cgatcgaaga ggccgtccgg tgggaaccgc
     3361 cgctgctgac catcacccgg gtcgccaccc gcgacaccga actggcgggt gtggctatcc
     3421 ccgcgggctc ggcggtcatg ccgatgctgg gcgccgccaa ccggcaggac gaccgatacc
     3481 ccgaacccga ccgcttcgac atcttccggt cgcccagagc ccatatcggt ttcgggcacg
     3541 gtgtccatgt ctgcctcgga atgcatctgg cccggctgga gatgcgcgtg gcgctcaact
     3601 tgctgttcga ccggttggcc aacctgcgtt tcgaccccgg ggccgacgat ccacacattc
     3661 gcggccaggt ctttcgctcg ccgacgtcgc ttccggtggc attcgatccg tgcgcctgag
     3721 cgccgagatt gccgtggcgg ctgcgatttt ggcggggacc gcgaccatag ccgcaatctc
     3781 gctaccaaga ttgagaccca ggagtccttg tgagtgattt cgaatcggtc gatttcttca
     3841 ccgacgcgtc gctggtcccc gatccctacc cgtatttcga ccagctgcgt gagcgttgtc
     3901 cggtcatgtc acggcccggg cagggcgtgg tggccataac ggggcaccag gaggctctcg
     3961 cggtgtacaa agacccggcg ttctcctcct gcgtcgcggt cgcgggccca ttttcgggac
     4021 tgccgttcga gccccacggg gacgacatcg gtgcgctgat cgagcaacat cgttcccaga
     4081 tcccgatgag tgaacacatc gtcacgcagg atcctccgga gcacacccgg acccgcggac
     4141 tgctcagccg cctgctgacg cccaagcggc tcaaggaaaa cgaagaattc atgtggcggc
     4201 tcgccgatgc gcagctcgac gagttcgtgt cccgccgaac ttgtgagttt ctgaacgact
     4261 acgcacgacc cttcaccggg ttggtgatcg ccgacctgtt gggagttccc ccggaagacc
     4321 atgacgagtt ccgcgccgtc tttttcggca aggtgtcggg tggaatcgag gacgattcga
     4381 tcacccgtgc ccacaatccg ctcgagtacc tcgacgacaa gttcaccacc tatatcaccg
     4441 agcgacgccg cgagccacga tccgacgtgc tgaccgagct ggccctggcg aagtaccccg
     4501 acggctcgac tccagaagtc atcgacgtcg tccggctggc cacgttcgtg ttcgcggccg
     4561 gacaggagac caccacgaag ttgctcagct tcgccgtgcg aatgatcgcc gaaaatccgc
     4621 agattcaaac gttgcttcgc gaagaccgca gcaagatacc cactttcgtc gaggaaacgc
     4681 tgcgcacgga aagtccggtc aagtgtcact tccggatggc gcgcacagcg acatcgattg
     4741 gcgacaccaa gatcgcggcc ggcagcacgg tgatgctgct acccggcgcg agcaatcggg
     4801 acgcgcgaaa gtttgaggag cccaacgagt tccgcatcga tcgcgcgaac gtccgcgagc
     4861 atgtggcgtt cgggcggggt aaccattcct gcccgggcgc gccgctggcc cgcgcagagg
     4921 gacgaatttc cctcaaccgc atcctcgacc ggatgtcagg catcggcatt ctcgaaacgg
     4981 agcacgggtc acccgacgcc cgccgctaca cctacgagcc gacctggcag atgcggggcc
     5041 tcaccgagct gcacctcgcc ttcagcccga ctgggtgagc acacctaata ctgccgactg
     5101 ccaccccgct ccccgccctc ccaacgcgtt ggttgacaat cgaggcaggc cccacttcgg
     5161 aacgtcgaag tcctagcgtc aaaggagaca caaccatggc tgaagccgtc atcgtcgaag
     5221 caatccgctc accgatcggc aagcgcaacg gcgggttgtc gggggtgcat ccggccgacc
     5281 tgtccgcgca ggtcctcaac gggctggccg acaaggccgg catcgacccg gggattgtcg
     5341 aggacgtcat ctggggctgc gtgatgcagg ccggtgaaca agccctcgac atcgcccgga
     5401 ccgcggtgct ggccgccggc tggcccgaga ccgtgcccgg cgtgacggtc gaccgccagt
     5461 gcgggtcgag ccagcaatcc atccacttcg ccgcggccgg cgtggtagcc gggcactacg
     5521 acgtcgtcgt cgcgggcggg gttgagtcga tgtcacggat cccgatgggc gcgtcgctcg
     5581 cgggcggggg ccggccgtat ccggagacct tcatgacgcg ctatgagggc agggtcccca
     5641 accagggcct cggcgcggaa atgatcgctg agcagtgggg cctcgaccgg ctcgcgctgg
     5701 acgagttcgc tctggcttca cacgaaaagg ccgcagcagc acaggattcc ggtgccttcg
     5761 acgaccagat cgtgggcatc aaggaccaag acagtaacgt cgtgctcaag gatgagggaa
     5821 tccggcgcgg cactccgatg gagaagatgg cggcgctgaa gccggccttc aaggaagacg
     5881 gtgtgatcca cgccggcaat tcctcgcaga tctccgatgg ctcggccgcg ctgctgttca
     5941 tgtcggccga gaaggccaag tcgctggggc tgaagccgat cgccaaggtg cacaccgcga
     6001 ccctggccgg agccgacccg gtgatcatgt tgaccgcgcc cattccggcg acccagaagg
     6061 tgctcaagaa atccgggctg agcatcgacg acatcggcgc atacgaggtc aacgaggcgt
     6121 tcgcgccggt cccgctcgcg tggctgaaag acatcggcgc cgacgagaag aagctcaacc
     6181 ccaacggcgg cgcgatcgcg ctcggtcacc cgctcggcgg ttcgggagcg cgtatcatga
     6241 ccacgctgct ttaccacatg cgggacaagg gaattcgcta cggcctgcag acgatgtgcg
     6301 agggcggtgg ccaggccaac gcgaccatcg tcgagctcct gtgacagatc ccgccgcggc
     6361 gccggcggcg ctcgtcgatc gctccggcaa cgtcatggtc attacgatca atcggccgga
     6421 cgcgcgcaac gcggtgaacg ccgcggtcag cattggggtg ggagatgcgc tggaggaagc
     6481 gcaacgcgac cccgacgtgc gggcggtggt gatcaccggc gcgggagaca agtcgttttg
     6541 cgccggagcc gatctcaagg cggtcgcgcg ccgggagaac ctgtatcacc ccgaccatcc
     6601 cgagtggggc ttcgccggtt acgtgcacca cttcgtcgac aagcccacca tcgccgcggt
     6661 caatggcacc gcgttcggcg gtggcacgga gttggcgttg gccagcgacc tggtagtggc
     6721 cagtgagcgc gcccaattcg ggctaccgga ggtcaagcgc gggctgatcg ccgctgcggg
     6781 cggggttttc cggatcgtca gccagctgcc ccgcaaagtt gcgatggagc tgctcttgac
     6841 cggtgagcca ctggcggctt ccgacgcctt ggagtggggc ctggtcaacc aggtggtgaa
     6901 ggacgggtcg gtcctggacg cggcactggc actcgcggcg cgtgtcaccg tcaacgcgcc
     6961 gctttccgta caggccagca agcgcatcgc gtatggggtc gacgacggcg tgatcgccgc
     7021 cgaggaggtg ggctgggatc gcaccctgcg cgagatgcgt tccctgatca ggtccgaaga
     7081 cgccaaggag ggaccgttgg cgttcgccga gaaacggcag ccggtctgga aggcgcgctg
     7141 accaaggggc ccgaagggtc agaccgccaa cctgttcacc gcggtccgga acacggcggg
     7201 cagtgccgta caggccggca cgagggcgcg gcgcgtcacc cgcggcgtgc tggccagcac
     7261 cagcccccgg gtgagcatcc ggtaatcgcg ggtaacccgg tgccacgcac cctcatacga
     7321 cgatggttcg tcgttcacga tggcgttgac cgccgcggcc gcttgcttga ccgcgagact
     7381 gatgccttca ccagtcagcg catcctcgta ccccgcggcg tcgcccacca acagcacccg
     7441 cccggcaacg cggcgggaga ccacctgccg catcggtccg cagccgcggg cgtgcccgcg
     7501 gctcgcgccc cgcagatgtt gggcgagcga cgggaaccag gcaaggtcgg gttgcccgcg
     7561 agacaggatc gccacgccga ccaggtccgg ttccaccggc gtcacgtacg cctcgcccca
     7621 acgcgaccag tgcacctcaa cgaactccga ccacaccggc accttgtaat gccagcgcac
     7681 gccgtagcgc cgcggtgttc cggccgtcgt ttggatgccg acggcgcgtc gaaccgtgga
     7741 atgcagtccg tcggccgcca ccaaccattt cgcgcggacg ccggccgccg tcactccgtg
     7801 cgcgtcttgc tcgacggttg tcacccgctc ctgaatccat tcggcgtctt gctctttggc
     7861 ccgtgcggcc agcgccgcgt gcagggtggt gcgtcgcacg ccccggcccg ggccgttgcg
     7921 aaaccgcgcc tcggcgcggc ggtgttcgct cagataggca attccgcgaa acggcatgcc
     7981 ggccgggtcg acgccgagcg aagtcagttc ggcgagtccg cccggcatca gcccttcgcc
     8041 gcacgccttg tcgatggggc tctcccgcgg gtcggcaacg atgaccgaaa gtccaagtct
     8101 ccgagcgtgt aacgcggtgg cgaggccgcc gggtccaccg ccgacgacca gcaggtcggt
     8161 gtcgtagtct gtcatgcgta tcccaaagcc gagttctcca cgcgcagacg cactctcagc
     8221 aacatggcgt tggccagtgt gaaaatgagc gcggtcagcc atgcggtgtg caccaatgga
     8281 agcgcaattc cttcggccac cacagcaaca tagttcgggt ggtgcagcca ccggtaaggc
     8341 ccgcgtttta tcaggggcgc ctccggcaac acgatcacgc gagtattcca ccgtgggccc
     8401 agcgtcgtga tgcaccacca gcgcagcacc tgggccagtg cggccacggc gagcatcggc
     8461 cagccgagcc agccgatgaa cggccggtgc agcgcccacg gttcgatgag gcagccgagc
     8521 agcagtgcgg tgtgcagagc gaccattacc ggatagtgat cttgaccaaa ctccttgccg
     8581 ccgtgggcaa aagcccatcg cgcattgcgt ttggctacga tcagctcggc aagccgttcg
     8641 acggctaccg ccaggatcag caagtaatac atagctcttc tgggccctac cagctcagca
     8701 ggacgaattc ggcggagaag cccggtccca tggcgatcat cagcccgatc gtacccggcg
     8761 acggcgggtc ggccatcgtg gcgtgaagta tgtcaagcac cgaaaccgat gacaggtttc
     8821 cattgtcgcg caacgaggtt ctggtgcgtt cgagggcatc cgccggcagc tccaacgcgt
     8881 cttcgaccgc gtcgatcacc ttcgggccgg cggcgtgcaa caaccacgtt gagacgtcgg
     8941 atgtggtcaa tccgtggtgg gcgaggaact tgcggatgtc gtcgcccacg tacttctcga
     9001 cgacggtcga gatctcggcg gacaggacaa tctgaaaccc gtcgccgccg atgttccagc
     9061 ccaacgcgtc ttcggtgtcc gggtaggtca tgctgcgggt ggccagcatc ttcggcccaa
     9121 ccggtgtccg gtcggctccg gtcgcgatga cgacggcggc gccgtcacca aacaggctgg
     9181 aagcgacgag attggcgatc gaaacatcct cgcgctgaat ggtcagcgag cacagctcga
     9241 ccgccagcag caccgccacg tgatcgggaa acgcccgaag gtagtcatgc acgcgcgcca
     9301 ggccggccgc gccggccaca cagcccaggc cgaacaacgg gacgcgtttg acgtcttggc
     9361 gcaacccgac gcgcgttgcc agccgggcct ccaggctcgg cacggcgagc ccggtaaccg
     9421 tcgtcgaaaa gacgacgtcg acctccgacg ggtcgagatt ggcctgatca agcgctgtga
     9481 gcagcgcctg ctcggccagg tccagggcga cctcgacaaa gttgtcgttg gcctcggtga
     9541 aaccggtcag ttccttgtat cgcgtcagtg gtagcgccgt gtgtcgggtg gtaactccgc
     9601 tgctcatcgc aaaccgccga aactccgggc cggcgaagtc ggtcaacgcc ccgatgacct
     9661 cttcctggct gtataggttc ggcgggaact tgacggcaac tgcggcgacg gacggatctg
     9721 cattggccgt gggtcgcttc aaaccgacgc gcgagaatct gtcccctgtg atggtatcca
     9781 tgtatcgggt tacggtgccg taggtagatc ggttcaaatc cgcgcgtaaa cgagacgtaa
     9841 cgacgtcgac cggacaactc ccgcggcggg catcgtcgcc cggttagggt cgggggtatg
     9901 cggattctcg tcaccggtgc caccggttat gggagatggc ggctcgtcac tggaggcagc
     9961 gctgccactg cccccgggac tggccgggga gcgctgcgaa ccaggctggc catgccgctc
    10021 gcattgacct c
//
LOCUS       DASSGQ010000146         4541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1226147,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000146 DASSGQ010000000
VERSION     DASSGQ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226147"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1169)
                     /locus_tag="VFI55_06615"
     CDS             complement(3..1169)
                     /locus_tag="VFI55_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HET7073738.1"
                     /translation="MRDVLAELMSIWRAGDTAGLGTVVRTFRSAPRPPGASMLVAPDG
                     SVTGSVSGGCVEGAVYELATEVVQTGTPRLERYGVSDGDAFAVGLTCGGMIDIFVEAI
                     SQAAFPELGAVADDVAQHRSVAIATVVAHRDPRWVGRRLVVRPDPNSPVAGSLGSARA
                     DAAVADDARGLLAVGRSEVLEYGLDGQRLDEGMEVFVSSCAPRPRMLVFGAVDFAAAL
                     AKQGAFLGYRVTVCDARAVFATPTRFPTADHVVVDWPHRYLAAQAEAGAVDESTVICV
                     LTHDPKFDVPVLEVALRLGVGYVGAMGSRRTHDDRMNRLRAAGLTDAELSRLSSPIGL
                     DLGARTPEETAVSIAADIIARRWGGGGRPLTQIVGRIHHDAQVEGELKDYLTQR"
     gene            1205..2179
                     /locus_tag="VFI55_06620"
     CDS             1205..2179
                     /locus_tag="VFI55_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7073739.1"
                     /translation="MTPAQLRAYSAVVRLGSVRAAAAELGVSDAGVSLLVTALRKELD
                     DPLFTRTGAGLAFTPGGLRLASRAVEILGLQQQTAIEVTEAAHGRRLLRIAASTAFAE
                     HAAPGLIELFSSRADDLSVELSVHPTSRFRDLICSRAVDIAIGPASENSVGSLGPDDT
                     IFVRPFLKYQIIAAVAPNSPLAVGVPTPALLRQQQWMLGPSAGSVGGEIATMLRDLAI
                     PESQQRIFQSDAAALEEVQRVGGVTLTIGFAVSKDLAAGRLTHLTGPGLDRSGQWCAA
                     TLAPSARQPAVSELVRFITTPRCIQAMIRGSGVGVTRFRPKVHVTLWS"
     gene            complement(2289..2945)
                     /locus_tag="VFI55_06625"
     CDS             complement(2289..2945)
                     /locus_tag="VFI55_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003900914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET7073740.1"
                     /translation="MSETGHEPGPTEWGIPAGGVGPWLGDPPDDPRYDPNLLRDGDTR
                     NVVDAYRYWTRDAIIADIDRRRHPLHVAIENFGHDANIGSVVRTANAFAVHTVHIVGR
                     RRWNRRGAMVTDRYQRLCHHDSTAELLEFAAGAGLTVVAVDNVPGATRLEETALPREC
                     LLVFGQEGPGITDGTRSGATLTVSIAQFGSTRSINAGVAAGIAMHAWIRQHGDLSRAW
                     "
     gene            complement(2942..3850)
                     /locus_tag="VFI55_06630"
     CDS             complement(2942..3850)
                     /locus_tag="VFI55_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073741.1"
                     /translation="MRTFIAVAGIVACCLAGCSGASQSVRPYGAQGARLGESLALLGW
                     NMSVSNLRWDGDYVLIDVSASVASANDPRAPHTKPEDIRFGLYGALAHPMESTALGSC
                     DDAMASVHDIGSPLSAPPDRLNGTVCLGPLKDRSQVRGVYSYSPRDRIPNSSAAYPAA
                     FPVGLMPTNANDTGLVVKTTSLSAWRADGTPVTKAQLGDPGAFTGNGYMLLGLEADAV
                     AARYRDDSAARGGPMMLLASPTQPGRGSNPACATYGSSVLILPDASLDSVHVNASLCT
                     QGEINEALLYATVAIDGTHAGVWTQR"
     gene            complement(3869..4408)
                     /gene="pyrE"
                     /locus_tag="VFI55_06635"
     CDS             complement(3869..4408)
                     /gene="pyrE"
                     /locus_tag="VFI55_06635"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET7073742.1"
                     /translation="MAGPDLVELAELVRRLSVVHGRVTLSSGKEADYYVDLRRATLHH
                     RASALIGTLMRELTSDWDYALVGGLTLGADPVATAVMHAPGRPIDAFVVRKSAKTHGL
                     QRLIEGSDVVGKRVLVVEDTSTTGASALTAVHAVQGAGGEVVGVATVVDRATGAAEAI
                     QAEGFPYRSVLGLADLGLG"
     gene            complement(4395..>4541)
                     /locus_tag="VFI55_06640"
     CDS             complement(4395..>4541)
                     /locus_tag="VFI55_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HET7073743.1"
                     /translation="ESLAEIARGTLIIIPSLQYKAIVAAGRLIPRNLMRAAGRRLGGG
                     RGRT"
BASE COUNT          698 a         1620 c         1544 g          679 t
ORIGIN      
        1 cgtcaacgct gagttaagta atcctttaac tcgccctcga cctgcgcgtc gtggtggatt
       61 cgccccacga tctgggtcag tggacggccc ccgccacccc agcgccgggc gatgatgtcg
      121 gcggcgatcg agaccgccgt ctcctccgga gtgcgggcgc cgaggtccag cccgatcggg
      181 ctggacagcc ggctcagctc ggcgtcggtc aggcccgcgg cacgcagccg gttcatccgg
      241 tcgtcgtggg tgcggcgcga ccccatcgcc cccacatatc ccacgccgag ccgcagcgcc
      301 acttcgagca caggcacgtc gaacttcgga tcgtgggtga gcacgcagat caccgtgctc
      361 tcgtcgacgg cacccgcctc ggcctgggcg gcgagatacc gatggggcca gtcgacgacg
      421 acgtgatcgg cggtcggaaa gcgcgtcggc gtggcgaaga ccgcgcgggc gtcgcagacg
      481 gtgacccggt agccgaggaa cgctccctgc ttggccaggg cggcggcgaa gtcgacggcg
      541 ccgaacacca gcatccgcgg gcgcggcgcg cagctggaca cgaacacctc catgccctcg
      601 tccagccgct gcccatcgag tccgtactcg aggacctcgc tgcggcccac cgccagcagg
      661 ccacgcgcat cgtcggcgac ggcggcatcg gcgcgcgccg aaccgagtga accggccacg
      721 ggactgttcg ggtccggccg gacgacgagc ctgcggccga cccaccgcgg gtcccggtgc
      781 gccacgaccg tcgcgatcgc caccgaacgg tgctgggcga cgtcgtcggc gaccgcgccc
      841 aattcgggaa acgccgcctg cgaaatcgcc tcgacgaaga tgtcgatcat gccgccgcac
      901 gtcaggccga ccgcgaacgc gtcgccgtcg ctgaccccgt agcgctccag tcgcggcgtc
      961 ccggtttgca ccacctcggt ggcgagctcg tagaccgcgc cctccacgca accgcccgac
     1021 accgaccccg tcaccgaacc gtccggcgcc accagcatcg aggctcccgg cggccgcggc
     1081 gcggaccgaa aggttcgcac caccgtcccc agccccgcgg tgtcaccggc acgccagatc
     1141 gacatcagct cggccaacac gtcccgcacc ctcccaaagt aggcccagac gtaagctcac
     1201 tgctatgact ccggctcaac ttcgggccta ttcggcggtg gtccgcctgg ggtcggtccg
     1261 agccgcggcc gcggagctgg gtgtttccga tgccggggtt tcgctgttgg tgacggcgct
     1321 gcgcaaggag ctcgacgatc cgctgttcac caggacgggt gcggggctgg cgttcacgcc
     1381 cggcgggctg cggctggcca gccgggctgt cgagattctg ggtctgcagc agcaaaccgc
     1441 gatcgaagtg accgaggccg cccacggacg tcggctgttg cgcatcgccg cgtccaccgc
     1501 gttcgccgaa cacgccgcgc ccggcctgat cgagctgttt tcgtcgcggg ccgacgacct
     1561 gtcggtggag ttgagcgtgc atccgacgag ccggttccgc gacctgatct gttcacgcgc
     1621 cgtcgacatc gcgatcggcc cggcctcgga aaactcggta ggttcgctgg gtcccgacga
     1681 cacgatcttc gtgcggccct tcctgaagta tcagatcatc gccgccgtgg cgcccaatag
     1741 tccactggcc gtgggcgttc cgacgccggc cctgctgcgc cagcagcagt ggatgctcgg
     1801 cccgtcggcc ggcagcgtgg gcggcgagat cgcaaccatg ttgcgcgact tggccattcc
     1861 ggagtcacaa caacggatct tccaaagcga cgccgcagcc ctcgaggagg tgcagcgggt
     1921 cggcggggtg acgctgacga tcggtttcgc ggtctccaaa gacctggccg ccgggcggtt
     1981 gacacatctg acgggccccg ggctggacag gtcgggccag tggtgcgcgg cgaccttggc
     2041 gccgtcggcc cgccagcccg ccgtctccga gttggtccgc ttcatcacca ccccgaggtg
     2101 tattcaggcg atgatccggg ggtccggggt cggggtgacg aggtttcgcc cgaaggtaca
     2161 tgtcacgttg tggagctaag cgtgatcgag tgtgcgcctg cggcgttggg tgtgcgccct
     2221 gggcgggaat atcgcggaaa tcccgccgct acttcacact gaaggccgcc aaagcacact
     2281 cgatacgact accaggcacg ggacaggtca ccgtgctgac ggatccacgc gtgcatcgcg
     2341 atcccggcgg cgacgcccgc gttgatgctg cgcgtcgaac cgaactgcgc gatcgacacc
     2401 gtgagcgtgg cgcccgaccg ggtgccgtcg gtgatgccgg gaccctcctg accgaacacc
     2461 agcagacact cccgcggtaa cgccgtctcc tccagacgcg tcgcccccgg gacgttgtcc
     2521 accgcgacca cggtcaaacc cgcaccggcg gcgaactcca gcagctcggc ggtgctgtcg
     2581 tgatggcaga gccgctggta gcgatcggtg accatggcgc cgcgccgatt ccagcgccgc
     2641 cgcccgacga tgtgcacggt gtgcacggca aaggcgttgg cggtgcgcac caccgagccg
     2701 atgttggcgt cgtggccgaa gttctcgatc gcgacgtgca gggggtgacg acgccggtcg
     2761 atgtcggcga tgatcgcgtc ccgggtccag tagcggtagg cgtcgacgac gttgcgagtg
     2821 tcgccgtcgc gcaacaggtt tggatcgtat cgcgggtcgt cgggcgggtc gcccagccac
     2881 ggcccgacgc cgccggccgg tatcccccat tcggtgggcc cgggttcgtg ccccgtttca
     2941 ctcatcgctg cgtccacacg ccggcatggg tgccgtcgat cgccacagtc gcatacagca
     3001 acgcctcgtt gatttcgccc tgggtacaca acgacgcgtt gacgtgcacc gagtccagcg
     3061 acgcgtcggg caggatcagc accgaggatc catacgtcgc gcaggcgggg ttcgatccgc
     3121 ggccgggctg ggtcggcgac gccagcagca tcatcgggcc accccgggcg gcggagtcgt
     3181 cgcggtagcg ggccgcgacg gcgtcggctt ccaagcccag cagcatgtag ccgttgccgg
     3241 tgaacgcccc cgggtcaccg agttgcgcct tggtcaccgg ggttccgtcg gcgcgccagg
     3301 ccgacaggct ggtggtcttg accaccaggc cggtgtcgtt tgcgttcgtc ggcatcagtc
     3361 ccaccggaaa cgccgcgggg taggcggccg acgagttcgg tatccggtcg cgcggcgagt
     3421 agctgtacac cccacggacc tgacttcgat ccttcagcgg cccaaggcaa accgtgccgt
     3481 tgagccggtc gggcggcgcc gacagcggag atccgatgtc gtgcacgctg gccatcgcgt
     3541 catcgcagct gcccagcgcg gtggattcca tcgggtgcgc cagcgcgccg tagagcccga
     3601 agcgaatgtc ctcgggtttg gtgtgcggcg cgcgcgggtc atttgccgac gccacactgg
     3661 ccgagacgtc gatcagcacg taatcgccat cccaccgcag attcgacacc gacatgttcc
     3721 agcccagcag ggccagcgat tcacccagcc gcgcaccctg cgcgccatac gggcggacgg
     3781 attggctggc gcccgaacac cccgccaggc agcaggccac gattccagcc acggcgatga
     3841 aagtgcgcac tgtgatccgg cgggcggcct agcccagtcc cagatcggcg agccccagca
     3901 cactgcggta cggaaatccc tcggcttgga tggcctcggc ggcgcccgtg gcgcggtcca
     3961 ccacggtggc cacgccgacc acctcgccac ccgcaccctg gacggcgtgc accgccgtca
     4021 acgcggaggc acccgtggtg ctggtgtcct ccaccaccag aactcgcttg ccaacgacgt
     4081 cggatccctc gataaggcgc tgcaggccat gggttttcgc cgacttgcgc acgacgaacg
     4141 catcgatggg acgccccggc gcgtgcatga cggccgtcgc caccggatcg gcgcccaggg
     4201 tcaggccgcc gacgagcgcg tagtcccaat cgctggtgag ctcgcgcatc agcgtgccga
     4261 tcagcgcgga ggcccggtgg tgcagcgtgg cacggcgcag gtcgacgtag tagtcggcct
     4321 ccttgcccga cgacagcgtg acccggccgt gcaccaccga cagccggcgc accagctccg
     4381 ccagctctac caggtcaggt ccggccacgg ccgccgccga gtcgtctgcc ggcggcgcgc
     4441 atcaggtttc gcgggatgag ccgcccggcc gcgacaatcg ccttgtactg gaggctcggg
     4501 atgatgatca gcgtgccgcg agcgatctcc gcgaggcttt c
//
LOCUS       DASSGQ010000147         6503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_739857,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000147 DASSGQ010000000
VERSION     DASSGQ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739857"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2770)
                     /locus_tag="VFI55_06645"
     CDS             complement(<1..2770)
                     /locus_tag="VFI55_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HET7073744.1"
                     /translation="MNSTQEELIEALRKSLKETERLKRENRQYLALFSEGPTEPVAVV
                     GMACRYPGGVDSPEALWQMVVDGRDVVSEFPGDRGWDLPGLFDPDPDAVGKSYTRCGG
                     FLADVADFDAAFFGIAPSEALAMDPQQRLLLEVSWEALERAGIDPLLLRGSATGVFAG
                     VFHGSYGGQGRVPGDLERYGLRGSTLSVASGRVAYALGLEGPAVSVDTACSSSLVAIH
                     LAAQSLRSGECGLALAGGVTMMATPAMFIEFSRQRALAADGRCKAYAGAADGTGFSEG
                     AGVLVLERLADARRLGHPVVAVLRGSAVNQDGASNGLATPNGPAQQRVIRAALANARL
                     GAADVDLVEGHGTGTMLGDPIEAQALLATYGQDRPADSPLWLGSIKSNMGHTSAAAGA
                     AGVIKVVQAMRHAVMPKTLHVDVPTPHVDWSAGAVSLLTDSRPWPAQDRPRRAGVSSF
                     GISGTNAHVIVEQAPSGLESAVAERDDAVVPWVLSARSADALVNQAARLLAHVNAHPD
                     LRVVDIAWSLVSTRSLFEHRAVLVGSDRDRLAAGLASLAAGEPGAGVVVGRAQPAGKT
                     VLVFPGQGSQWAGMGAELLDGSTVFAEHLHRCDKALAEHVEWSLIDVIRGVSGAPGLD
                     RVDVVQPALWAVMVSLAELWRSMGVVPDAVIGHSQGEIAAAYVAGALSLEDAARVVAL
                     RSRLLMPLSGRGGMVSMACGLSQARELVAAWGDRLNIAAVNGVSAVAVSGDVDALTAL
                     TRRCEDDNVRVRKIDVDYASHSVHVDVIREPLNQALNGLAPQSSSVAFFSTVTGELMD
                     TAGLDADYWYRSIRQTVRFDQAVGSACDAGYRVFVESSPHPVLIAGIEETLGDRGVAG
                     DAVVIPSLGRDDGGLQRLWRSVAQAHVVGVRVDWAAVFAGLGVCRVELPTYGFVRRRF
                     WLPLEVVGS"
     gene            complement(3080..4141)
                     /locus_tag="VFI55_06650"
     CDS             complement(3080..4141)
                     /locus_tag="VFI55_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HET7073745.1"
                     /translation="MSVIAGVFGALPPYRYSQRELTDFFVSIPDFEGFEDIVRHLHAS
                     AKVNSRHLVLPIERYLTLTDFGVANRIFIEKAVDLGCEALLGALDEAGLQPQDLDVLV
                     STTVTGLAVPSLDARIAGRLGLRDDVRRVPLFGLGCVAGAAGVGRLNDYLRGAPDDIA
                     ALVSVELCSLTYPGYKPSLAGMVGSALFADGAAAVVVVGERRAEQVGAAGPRIVDSRS
                     HLYPDSLRTMGYDVGSAGFELVLSRDVADVVREYLGNDVTAFLAAHGLTTTDVGAWVT
                     HPGGPKIIDAITESLNLPPQALELTWRSLGEIGNLSSASVLHVLRDTMAKPPPADSPG
                     LMMAMGPGFCSELVLLHWH"
     gene            complement(4247..5665)
                     /gene="argH"
                     /locus_tag="VFI55_06655"
     CDS             complement(4247..5665)
                     /gene="argH"
                     /locus_tag="VFI55_06655"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET7073746.1"
                     /translation="MSTNEGSLWGGRFADGPSDALAALSKSTHFDWVLAPYDVVASRA
                     HTIVLYRAGLLDEEQRDGLLAGLDSLAEDVADGSFGPVVTDEDVHAALERGLIDRVGP
                     DLGGRLRAGRSRNDQVATLFRMWLRDAVRRVAAGALEVVGALVVQAAAHPTAVMPGKT
                     HLQSAQPILLAHHLLAHAHPLLRDVDRIVDFDRRAAVSPYGSGALAGSSLGLDPDAIA
                     RDLGFAAAADNSVDATAARDFAAEASFIFAMIGVDLSRLAEDIIIWSSTEFGYVSLHD
                     SWSTGSSIMPQKKNPDIAELARGKAGRLIGNLAGLLATLKAQPLAYNRDLQEDKEPVF
                     DAVAQLELLLPAMAGLVASLTFNVERMAALAPAGYTLATDIAEWLVRQGVPFRSAHEA
                     AGAAVRVAERRGVGLEELTDDELVAISPELTPQVREVLTIEGSVSSRDARGGTAPTRV
                     AEQLQGVLASCAELRDALRLSN"
     gene            complement(5746..>6503)
                     /gene="argG"
                     /locus_tag="VFI55_06660"
     CDS             complement(5746..>6503)
                     /gene="argG"
                     /locus_tag="VFI55_06660"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET7073747.1"
                     /translation="YAWTREKAIRFAEENAIPINVTKRSPFSIDQNVWGRAVETGFLE
                     HLWNAPTKDIYAYTEDPTLNWSTPDEVIVGFERGVPVTIDGKPVSMLQAIEELNARAG
                     AQGVGRLDVVEDRLVGIKSREIYEAPGAMVLITAHTELEHVTLERELGRFKRQTDQRW
                     AELVYDGLWYSPLKAALETFVAKTQEHVSGEVRMVLHGGHIAVNGRRSAESLYDFNLA
                     TYDEGDTFDQSKAKGFVYVHGLSSKLAARRDRQ"
BASE COUNT         1176 a         2408 c         1966 g          953 t
ORIGIN      
        1 ccgacccaac cacctccaac ggcaaccaaa accgccgccg cacaaaccca tacgtcggca
       61 actccacccg acaaacaccc aaccccgcaa acaccgcggc ccaatcgacc cgcacgccaa
      121 ccacgtgtgc ctgcgccacc gaacgccaca accgttgcag cccaccgtca tcgcgaccca
      181 atgacggaat gacgacggcg tcgccagcga cgccgcgatc gcccaacgtc tcctcgatgc
      241 cggcaatcaa caccggatgc ggactggatt cgacgaacac tcgatacccg gcgtcgcacg
      301 cgctgcccac cgcctggtcg aatcgcaccg tctgccggat gcttcgatac cagtagtcgg
      361 cgtccaatcc ggcggtgtcc atcagctcgc cggtcaccgt cgagaagaag gcgaccgagg
      421 aagactgcgg tgcaaggccg ttcagcgctt ggttgagggg ctcacggatg acgtcgacgt
      481 gcaccgagtg cgatgcgtag tcgacatcga tcttgcgaac gcgaacgttg tcgtcctcac
      541 accgtcgcgt caacgccgtc agagcgtcca cgtcaccgga aacggccact gccgaaacac
      601 cgttaacggc agcgatgttc agccgatcac cccaggcggc caccaactcc cgggcttgcg
      661 acagcccgca cgccatcgaa accatgccgc cccggccgga caagggcatc agcagcctgc
      721 tgcgcaacgc caccacccgg gcggcgtctt ccagcgacag cgctccggcc acgtaggccg
      781 cggcgatttc gccctgcgaa tgcccgatca ccgcatcggg cactacgccc atcgatctcc
      841 acaactcggc gagcgacacc atgaccgccc acagcgccgg ctgcaccacg tcgacccgat
      901 cgaggcccgg cgcaccggac acaccgcgga tgacgtcgat caacgaccac tccacgtgct
      961 ccgcgagcgc cttgtcgcat cggtgcaggt gctccgcgaa taccgttgag ccatcgagta
     1021 actcggctcc catgccggcc cactgcgagc cttggcccgg aaacaccagg acggtcttgc
     1081 ccgccggctg tgcccgaccg acgaccacac cggcgcccgg ctcaccagcg gccaagctcg
     1141 ccaaccccgc cgccaaccgg tcgcgatcgg aacccaccag caccgcgcga tgctcgaaca
     1201 gcgaccgcgt cgaaaccaac gaccacgcga tatccaccac acgcaggtcc gggtgggcat
     1261 tcacatgcgc gagcagccgc gccgcctggt ttaccagcgc gtcagccgac cgcgccgaca
     1321 acacccacgg caccaccgcg tcatcgcgct cggccacagc actttccagg ccggatggag
     1381 cctgttccac gatcacatgc gcgttcgtgc cgctgatccc gaacgacgac accccggcac
     1441 gacgcggccg gtcctgcgcg ggccatggcc gcgagtcggt caataacgac accgcccccg
     1501 ccgaccaatc cacatgcggg gtaggcacat ccacgtgcag cgtcttgggc atcaccgcgt
     1561 ggcgcatcgc ttgcaccact ttgatcactc cggcggcgcc cgccgcagcc gatgtgtgcc
     1621 ccatgttcga tttgatcgac ccgagccaca gcggtgaatc cgccggccgg tcctggccgt
     1681 acgtcgccaa aagcgcttgg gcttcaatcg gatcccccaa catggtgccc gtcccatgcc
     1741 cctccaccag atccacgtcc gccgcgccca gccgcgcgtt ggccagcgcc gctcggataa
     1801 cccgctgctg cgccggccca ttgggcgtcg ccagtccgtt ggaggcgcca tcctgattca
     1861 ccgcagaacc ccgcagcacc gccaccaccg gatgccccaa ccgccgggca tcggccagcc
     1921 gctccagcac cagcacaccg gcgccttcgg aaaacccggt cccgtcggcg gcgcccgcgt
     1981 atgccttgca ccggccgtcg gcggccagcg cccgctgtcg actgaattcg atgaacatcg
     2041 ccggcgtggc catcatggtc acaccaccgg ccagcgcgag accacactcg ccggaccgca
     2101 gcgactgcgc cgccagatgt atcgccacca acgacgacga acacgcggta tccacggaca
     2161 ccgccgggcc ttccaacccc agcgcatacg ccacccggcc cgacgccaca ctcagggtgg
     2221 agccccgcag cccgtaccgt tcgaggtctc ccgggacccg cccctggccg ccataggagc
     2281 cgtggaacac tccggcgaac acgcccgttg ccgaaccgcg cagcaacagc ggatcaatcc
     2341 cggcccgctc caacgcttcc cacgacacct ctaacagcaa gcgttgctgg ggatccatcg
     2401 ccagcgcctc gctgggtgcg atcccgaaaa acgcggcatc gaagtccgcc acgtccgcca
     2461 ggaagccgcc gcaccgcgta tacgacttgc ccaccgcgtc gggatccggg tcgaacaggc
     2521 ccggcaaatc ccagccccga tcgcccggaa actccgacac cacgtcgcgg ccgtcaacga
     2581 ccatctgcca caaagcctcc ggggaatcca cccctccggg atatcggcac gccatcccca
     2641 ccaccgccac cggctcggtg ggcccctcgg aaaacagagc caggtattgg cggttctccc
     2701 gcttcagccg ctcggtttcc ttcagcgatt ttctgagggc ctcgatcagc tcttcttggg
     2761 tgctgttcat gagaccccgc ctcccatgtc actcatacga acgggtgtga tgccgtccga
     2821 tggatgcagc gataccttcg caacggccgc ggataacgcg tggggacgga gggtatcgac
     2881 ggccgaccgc gggtcatctg ccccggccgg cacaggtggc gcccttcgtc aaaccccgaa
     2941 aacccccgaa cacccccgag agtcgcccat cgccatcact tccctcgagg ccccaaacgc
     3001 ggccccaaac gacgctgcct tcacggtacg acgggatggc caccagcgca cataccccaa
     3061 acacctagtc gccgccggat cagtgccagt gcagcagcac gagttcggaa cagaaacccg
     3121 ggcccatcgc catcatcagc ccggggctgt cagccggcgg cggtttcgcc atggtgtcac
     3181 gaaggacgtg caacaccgac gccgacgaga gattgccgat ttcgcccagc gaacgccacg
     3241 tcagctccag ggcctgcggc ggcaggttaa ggctctcggt gatcgcgtcg atgatcttgg
     3301 ggccgccggg atgagtaacc caggcgccga cgtccgtcgt ggtgaggccg tgcgcggcca
     3361 gaaacgcggt gacgtcattg cccagatact cgcggacaac gtcggcgaca tcgcgggaca
     3421 gcacgagctc gaacccggcg ctgccaacgt cgtaacccat ggtgcgcagc gagtcggggt
     3481 acagatggct gcgcgaatcg acgatgcgcg ggccggcggc gcccacctgt tcggcgcgac
     3541 gctcgccaac gacgaccacc gccgcggctc cgtcggcaaa cagcgcgctg ccgaccatgc
     3601 cggccagcga cggcttgtac cccgggtagg tgagtgagca aagttcgacg gagaccagcg
     3661 ccgcgatgtc gtccggcgcg ccgcgcaggt agtcgttcag gcggcccacc cccgccgccc
     3721 cggccacgca accaaggccg aacagcggca cccggcgcac atcgtcgcgc aacccgaggc
     3781 gcccggcgat ccgggcatcc agcgacggga ccgccaggcc ggtcaccgtc gtcgagacca
     3841 gcacgtcgag gtcctgaggt tgcaggcccg cttcgtcaag ggcacccaag agtgcctcgc
     3901 agccaagatc cacggccttt tcgatgaata ttcggttcgc gaccccgaag tcggtgagcg
     3961 tgagataccg ctcgatcggc agaaccaggt ggcgactgtt gaccttggcg ctggcatgca
     4021 agtgccggac gatgtcttcg aaaccctcga aatcgggaat gctgacgaaa aagtcggtca
     4081 gttctcgctg ggagtagcgg tacggaggca gcgcaccgaa cactcctgcg atgacgctca
     4141 tggatcccac cccttggtga cgaagatcga gtccgaaagg ttcaaccgga tcgcttacgg
     4201 caaattttag tcggcgtcaa agctggccga cgcggggacc gacgtctcag ttgctgaggc
     4261 gcagcgcgtc cctcaactcg gcacagctcg ccaggacacc ctggagttgc tcggcgaccc
     4321 gagtcggcgc cgtgccgccc ctcgcgtcac gggaggagac cgagccctcg atcgtcagca
     4381 cctcgcggac ctgcggcgtc agctcgggac tgatggcgac cagctcgtcg tcggtcagct
     4441 cctccagccc gacgccgcgc cgctcggcga cccgcacggc agcgccggcc gcctcgtgcg
     4501 cggatcggaa cggcacccct tgccgcacaa gccattccgc gatatcggtg gccagcgtgt
     4561 aaccggccgg ggccagggcc gccatccgct cgacgttgaa ggtcaggctg gccaccagtc
     4621 ccgccatcgc cggcagcagc agctccagct gggcaacggc gtcgaacacc ggctccttgt
     4681 cttcctgcag gtcacggttg taagcgagcg gctgggcctt cagcgtcgcc agcagcccgg
     4741 ccaggtttcc aatcagccgt cctgccttac cgcgagccaa ctcggcaatg tcggggttct
     4801 tcttctgcgg catgatcgag ctgccggtgg accaggaatc gtgcaaactc acatagccga
     4861 attccgtcga actccagatg atgatgtcct cggccagccg cgacaggtcg acgccgatca
     4921 tggcgaagat gaacgaggct tcggcggcga agtctcgggc cgccgtcgcg tcgacggaat
     4981 tgtcggcggc ggcggcaaaa cccagatccc tggcgatcgc gtcagggtcc agccccagcg
     5041 acgaaccggc cagcgcaccc gagccgtacg gggataccgc cgcacgcctg tcgaagtcga
     5101 cgatgcggtc gacgtcccgc agcaacggat gggcgtgggc gagcagatgg tgggccagca
     5161 ggatcggctg ggccgactgc aaatgggtct taccgggcat gacggccgtc ggatgcgcgg
     5221 cggcctggac gaccagtgcg ccgacgacct cgagcgcccc ggccgcgacc cgtcgcacgg
     5281 cgtcgcgaag ccacatccgg aacagggtgg ccacctggtc gttgcgcgac cgcccggccc
     5341 gcaggcgtcc gcccaaatcg ggcccgaccc ggtcgatcag gccgcgctcg agcgcggcgt
     5401 gcacatcctc atctgtgacc accggtccga agctgccgtc ggcgacgtcc tcggcgagac
     5461 tgtccaaccc ggccaagagt ccatcgcgct gctcttcgtc gagcaggccc gcccgataga
     5521 gcacgatcgt gtgggcccgc gaggcgacga cgtcgtaggg agccagcacc cagtcgaagt
     5581 gtgtcgactt gctcagcgcg gccagcgcgt cggacggacc gtcggcgaac cgcccgcccc
     5641 acagcgaccc ctcattggtg ctcacttttc gccatcacct cgccgcgact gtgaatctca
     5701 cgacgtgttc gcggcgtgtc gcgtcgtgag attcacactc gcgcctcact ggcggtcccg
     5761 tcgggcggcc agcttggacg acagcccgtg tacgtagacg aagcccttcg ccttggactg
     5821 gtcgaaggtg tcgccctcgt cgtaggtggc caggttgaag tcgtagagcg actccgcgct
     5881 gcgccgcccg ttgaccgcaa tgtggccgcc gtgcaacacc attcgcacct cgccggagac
     5941 gtgttcttgc gtcttggcga cgaaggtctc cagtgcggct ttcagcggcg agtaccacag
     6001 cccgtcgtac accagttcgg cccagcgctg gtcggtctgg cgtttgaagc ggcccagctc
     6061 acgctcgagg gtgacgtgtt cgagttcggt gtgtgcggtg atcagcacca tcgctcccgg
     6121 tgcctcgtaa atctcgcggc tcttgatgcc caccaggcgg tcctcgacca cgtcgaggcg
     6181 cccgacgccc tgcgcgccgg cacgggcgtt gagttcctcg atggcctgca gcatcgacac
     6241 cggtttgccg tcgatggtca ccggcacccc gcgctcgaat ccgacgatca cctcgtcggg
     6301 cgtgctccag ttgagggtgg ggtcttcggt gtaggcgtag atgtctttgg tgggcgcatt
     6361 ccacaggtgt tccaggaacc cggtctccac cgcgcggccc cacacgttct ggtcgatcga
     6421 gaacggcgag cgcttggtga cgttgatcgg gatcgcgttc tcttcggcga acctgatcgc
     6481 cttctcccgc gtccaggcgt agt
//
LOCUS       DASSGQ010000148        21453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_314570,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000148 DASSGQ010000000
VERSION     DASSGQ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 21453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21453
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314570"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1201
                     /locus_tag="VFI55_06665"
     CDS             86..1201
                     /locus_tag="VFI55_06665"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HET7073748.1"
                     /translation="MSKNTITLTPEQADAFGRELDAIKERVTADLGEKDAEYIRRVIK
                     TQRALELSGRALLFAGFLPPAWLLGTAMLGVSKILDNMEIGHNIMHGQYDWMRDPTIS
                     GRSFEWDTACPADQWRHSHNYMHHTHTNIVGMDRDIGYGILRMSEDQPWEPYFLGNPV
                     YAFLLMVLFQYGVALHEMETERIRSGEIRLRDKREVLRAIWKKTRRQTLKDYVAFPLL
                     AGPFAPFVLAGNLTANLMRNVWSYMIIFCGHFPDGTQEFTVEETKDESRGMWYFRQVL
                     GSANLTGGKLFHLMSGNLSHQIEHHLFPDLPARRYGEIAPEVQEICERYGIPYNRGPL
                     PRQFATVVRKIVRLSFPDSWLRKSEVGKPADRVPVAA"
     gene            1276..1404
                     /locus_tag="VFI55_06670"
                     /pseudo
     CDS             1276..1404
                     /locus_tag="VFI55_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(1394..2437)
                     /locus_tag="VFI55_06675"
     CDS             complement(1394..2437)
                     /locus_tag="VFI55_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7073749.1"
                     /translation="MTPTMRAERFYADTKKIALEDVPIPQPGPGEVLVKVAFCGICHS
                     DLSLINGTFPAQAPVVTQGHEASGIIAEVGPDVSGWAEGDRVVVAAGRPCQKCPNCRR
                     GDVMNCLRIQLMAFAYDGAWAEYTVAQAAGLTRVPDNVPLEQAAILADAVSTPYGAVV
                     RTGKVSVGESVAVWGVGGIGTHIVQLARLVGAVPVIAIDINPAVMDRALAVGADYSFD
                     SRDDALRDKIAEITGGRLLDVAFDAVGLKATFEQALGCLTSAGRLVAVGMSAEAPTIG
                     PTSMFGLTRKQVLGHLGYQNVDIETLAKLVSAGRLDLSRSISEIVALEDISLGIEKLE
                     RQDGNPIRILVKP"
     gene            complement(2443..2655)
                     /locus_tag="VFI55_06680"
     CDS             complement(2443..2655)
                     /locus_tag="VFI55_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HET7073750.1"
                     /translation="MRLSTRNQLTGTITEVDLGSVMAIVKVKLDGGGQIVTSSVTKDA
                     ATSLGLTVGQPATVFIKSTEVTIGVE"
     gene            complement(2812..3429)
                     /locus_tag="VFI55_06685"
     CDS             complement(2812..3429)
                     /locus_tag="VFI55_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7073751.1"
                     /translation="MTAAADDAVLGSPPATNDPFRLRLLDGLATSIGERGYRASTVAD
                     IVRHARTSKRTFYDQFASKEQCFLELLRADIEKLGESIQVAVDPDADWHQQIRQAVEA
                     YVGYIESRPAITLSWIRELPSLGAVARPVQRRGLQLLSNLLIDLSASPGFRRADLPPL
                     TAPLAVILLAGLRELTALAVEDGRPTREIVAPAVDASIALLGPRH"
     gene            3572..4909
                     /locus_tag="VFI55_06690"
     CDS             3572..4909
                     /locus_tag="VFI55_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7073752.1"
                     /translation="MSEVLSSPPAPLAVRLPPATRIPKVVCGLLFATSRRGMIKRVTR
                     RYGNVFTLNVPIYGRVVVVGDLQLAKQVFTTSPDELGNIQPNLSRLFGSGSVFGLDGD
                     DHRRRRRLLAPPFHGKSMKNYEAIIEEETLRETAGWPEGRPFATLPSMMRITLNAILR
                     AIFGADGAELDELRRLIPPWVTLGSRVAPLPKPKRNYGRFSPWARLDEWRGQYDVVLD
                     KLIAAERADPNLADRTDVLALMLRSTYEDGSAMSRKDIGDELLTLLAAGHETTAATLA
                     WAFERLSRHPDVLAALVEEADNGAQGGGNELRQATILEVQRARTVIDFAARRVYQPAY
                     QLGEWVIPHGDSIIVGISQIHDDPDVFADPGRFDPQRYIGGKPSAFAWIPFGGGTRRC
                     VGAAFANMEMDVVLRTVLRNFTIETTTDPGERWHCRGVAYTPKNGGRVVVRPR"
     gene            complement(4916..5431)
                     /gene="msrA"
                     /locus_tag="VFI55_06695"
     CDS             complement(4916..5431)
                     /gene="msrA"
                     /locus_tag="VFI55_06695"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011742283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET7073753.1"
                     /translation="MTNTQKAILAGGCFWGMQDLIRKQPGVLSTRVGYSGGDTPNATY
                     RNHGTHAEAVEIVYDPSVTDYRALLEFFFQIHDPTTKNRQGNDRGTSYRSAIYYLDDE
                     QRRIALDTIADVEASGLWPGKVVTEVSPAGEFWEAEPEHQDYLERYPNGYTCHYVRPG
                     WKLPRRATAGQ"
     gene            5560..6060
                     /locus_tag="VFI55_06700"
     CDS             5560..6060
                     /locus_tag="VFI55_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7073754.1"
                     /translation="MSTRGFSRSELSAAFEKFEETVARAAKTRDWDAWVEQYTPNVEY
                     IEHAAGTMRGREQVRDWIQRTMTTFPGSHMVAFPSLWSVIDEPTGRVICELDNPMADP
                     GDGTVVSATNISIITYAGDGQWRRQEDIYNPLRFLRAGMKWCRKAQALGTLDDDAARW
                     MRENGG"
     gene            6081..7130
                     /locus_tag="VFI55_06705"
     CDS             6081..7130
                     /locus_tag="VFI55_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7073755.1"
                     /translation="MSTKPKLVVGANGFLGSHVTRRLVADGAQVRAMVRPTANTRAID
                     DLSPTRCTRFHGDVFDTAALRAAMDGVDDVYYCVVDTRAWLRDPSPLFRTNVEGLRNV
                     LDVAVQQSGLRRFVFTSTYATVGRRHGHVATEEDVIGSRGLTPYVQSRVQAEDLVMRY
                     VADAGLPAVAMCVSTTYGSGDWGRTTHGAFIAGAVFGKLPFTMDGIQLEVVGVDDAAR
                     AMILAAERGRVGERYLISERMIALRDVVRIAADEAGVPPPRRSISVPMLYALGALGSL
                     KARLTGKDAELSLNSVRMMRAEADVDSSKAKRELGWQPRPVEVSIREAAQFWAALKGA
                     QTARRDTQNRTAQAE"
     gene            7150..7965
                     /locus_tag="VFI55_06710"
     CDS             7150..7965
                     /locus_tag="VFI55_06710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7073756.1"
                     /translation="MPVDLHGPPQTMLATLYAKALDADAPRSILNDHFAKAAVAQIDY
                     DWDKTTITPLRAPSVAVRTAHFDNWTRQFLAVHEQAVVLHVGCGLDARVYRVDPGPGV
                     WWYDVDYPDVIGLRERVYPGRANYRMLPASVTDPSWLADIAADGPTLFIGEGLTMYLS
                     KDDGLALLRRVVDRFPSGELQFDAFNTFGIRTQWINAVVRRSGSTLQWGIDGPGDIVD
                     AVAGVRLLVWESVFDSDTFDLVSPVARQVGRVMAVVPVLRTMAQYHRYAFGPA"
     gene            8041..8415
                     /locus_tag="VFI55_06715"
     CDS             8041..8415
                     /locus_tag="VFI55_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HET7073757.1"
                     /translation="MAHRQVLEPSAGHPITIEPTKGRVQVRVNGELVADTTAALELRE
                     ATLPVVQYIPLGDVVQDRLTRTDTSTYCPFKGDASYYSVTTADGYTVEDVIWTYEQPY
                     PAVAAIAGHVAFYPNKAEISVA"
     gene            complement(8412..8816)
                     /locus_tag="VFI55_06720"
     CDS             complement(8412..8816)
                     /locus_tag="VFI55_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7073758.1"
                     /translation="MTPFDGPHTELAWMFLQSLCEGGDLDEGFALLSDDFTYWSLFTR
                     VSFDKKTLRQAVNWRKKHLELGVDLLRCISEGGSVVVEGHATGTSAKGVAYDSPFACI
                     FEIRDGLIISMREYSDTRSQAEALPESPTRVY"
     gene            8906..9820
                     /locus_tag="VFI55_06725"
     CDS             8906..9820
                     /locus_tag="VFI55_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HET7073759.1"
                     /translation="MESARTVRLPGQLSFGHTLAPLRRGPRDPCFQAAGAEGAIWRTS
                     LLPTGPVTARISRAAPDAAHCVAWGSGAQEFIEMLPALLGADDDASDFVPRDPTVAAA
                     HRRVPHLRLGRTGLVLEALIPAIIEQRVPGADAFRSWRLLVSRNGTPAPGPAPAGMRV
                     PPSAEAWRNIPSWEFHRANVDPRRAQTVVSCARRAASLERLVTRTTAQAREALMSLPG
                     VGEWTAAETAQRAFGDADAVSVGDYHIPKMIGWTLLGRPVDDAGMLELLEPMRPHRHR
                     VVRLLEASGVASEPRRGARLPVQQIHSL"
     gene            complement(9882..11321)
                     /locus_tag="VFI55_06730"
     CDS             complement(9882..11321)
                     /locus_tag="VFI55_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET7073760.1"
                     /translation="MSEHVDDEPGPPEAPAPAAGGRFGASIRKSGYLQKWLLLGITIG
                     GVAGLGAVVFYLLLKYTSEFLLGYLAGYHIPTPVGEGGSHGSAGFQRPWAIPLVTTAG
                     ALLSALIVAKLAPEASGHGTDEAIEAVHADPRAIRFRVVLVKMVASALTIGSGGSAGR
                     EGPTAQISAGFSSLLTRRLNLSDEDGRIAVALGIGAGIGAIFAAPLGGAVLAASLPYR
                     DDFDYRCLLPGFITSGTAYAVLGAFLGFDPLFGYIDAEYRFERAWPLLWFVVIGLVAA
                     AIGYLYARTFHASVRLTQRLPGGPVLKPALGGLLVGLLGLLIPQILSSGYGWAQLAAD
                     RGSLTAIPLWIVIVLPFAKIVATSLSIGTGGSGGLFGPGIVIGAFVGAAVWRLGELSG
                     LPGVPDAPGIFVVVGMMTCFGSVARAPLAIMIMVAEMTGSFSVVPGAILSVGIAALVM
                     SRTNVTIYETQRLTRDTSAAAEGEPAVES"
     gene            11442..12425
                     /locus_tag="VFI55_06735"
     CDS             11442..12425
                     /locus_tag="VFI55_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET7073761.1"
                     /translation="MPAITADTITLPRVGPPGPGDTERPVRSITTGPRGYEGEGFPVV
                     RAFAGVSDAALDPFVHMDQMGEVDYEPGEPRGTDWHPHRGFETVTYMIDGKFAHQDSH
                     GGGGLIADGATQWMTAGSGILHIETPPAELVESGGLFHGVQLWVNLPKKDKFTAPRYQ
                     AIEGGDAKLLASDDGGALVRIIAGEIDGHRGPGVTHTPITLAHVTIENGGRLNIPWRR
                     DFNALVYVLSGSGYVGPVGHPIRSRQLAVLGPGDRITVGADRTQESTRTTSIDVLLLG
                     GQPIREPVFHYGPFVMNTREELIEALEDYQAGKFGNIPPNALMPHHGGGTL"
     gene            12428..13063
                     /locus_tag="VFI55_06740"
     CDS             12428..13063
                     /locus_tag="VFI55_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7073762.1"
                     /translation="MPPTAGRRPGRPPAAKADDTRKRILHAARQVFSERGYDGATFQA
                     IASRADLTRPAINHYFSSKRALYREVVAQANELVVAAGIERAACEVTLMARLTAFISD
                     AVRANSVNPSASAFLVTNVLESQRHPELVAAENDAVLITREFLTRAVNEAIERGEIVT
                     DIDASALVETLLVVLCGVGLYAGYVRTYEEMLAVTDTLRQLLEGALWRSEA"
     gene            13149..14090
                     /locus_tag="VFI55_06745"
     CDS             13149..14090
                     /locus_tag="VFI55_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7073763.1"
                     /translation="MTDLDASLPPSLRTAGDSWEITELVGATALGVAASRAVETAGPN
                     PLIRDDFAQILVSSAGPAWARLTDSELAWLDGDEHGQRVHRLGIAYQAVRTHFFDEYF
                     ADAVGAGIRQVVILAAGLDSRAYRLSWPAGTAVYEIDQPKVLEYKTGILESHGAVPTA
                     DRRPVPVDLRHDWPAALTAAGFDRTQPTAWLAEGLLPYLPSDAQDRLFEMFTALSAPG
                     SRVAVEAFGLNSKGNTQRWLRMRERLGLDVNVQALTYHEPDRSDAVQWLVDHGWQVRR
                     VNNREEMARLGRPVPDDLDDEAVRSTLLRARLGGPSD"
     gene            14135..15067
                     /locus_tag="VFI55_06750"
     CDS             14135..15067
                     /locus_tag="VFI55_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7073764.1"
                     /translation="MRTHDDTWDIKTSVGSTAVMVAAARAVETERPDSLIRDPYARLL
                     VTNAGANVMWEAMLDPSMVAKVEAIDAEAAATIQHMRSYQAVRTNFFDTYILAAAADG
                     IRQIVILASGLDSRAYRLDWPADTAVYEIDQPKVLAYKSTTLAENGVNPGAERREVPI
                     DLRQDWPAALRAAGFDPAARTAWLAEGLLMYLPAEAQDKLFTQVGELSAAGSRIAAET
                     SPLHGEDRRQQMRERFKKVTDTLGFEQAVDIQELIYHDEDRAVLADWLNGHGWRATAQ
                     SALDEMRRLGRSVDGVPMGDDKDAFAEFVTAERL"
     gene            15067..16008
                     /locus_tag="VFI55_06755"
     CDS             15067..16008
                     /locus_tag="VFI55_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7073765.1"
                     /translation="MARTADDSWDIATSVGATAVMVALARAAETASADPLIRDRFAEL
                     LVSTPELEGVREQVAAWWVPEPDDDSGEGTDPDLAVDAQQMIDYQAVRTHFFDAYFAG
                     AVATGIRQVVILAAGLDSRAYRLDWPDGTVVYEIDLPKVLEYKTATLAGQGAAPIADR
                     RPVAVDLRHDWPQALRDAGFDANQPTAWLAEGLLPFLPAAAQESMFASIDRLSGPASR
                     VAVEIFGVDEDKRREAEERWQQLRAKRAARGHDTSFNPFDLWFDDDGRPDSADWFTAQ
                     GWTSRTVNAREEALRLGRAPQSEDRPFTNSFVTATKP"
     gene            16128..17690
                     /locus_tag="VFI55_06760"
     CDS             16128..17690
                     /locus_tag="VFI55_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016894828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET7073766.1"
                     /translation="MSSSESGQPQRLVVLKGRLLASMASAFVMAITAIGWTGYHTAVG
                     KIIISHVLPNAQTPAGADQNILLMGLDSRLDQNGQPLPQEMYEALHAGDESSGGYNAN
                     VLIVVHISGGDGPVTAVSIPRDDYVDLSGCPGSVCKGKVKQAYGLAYQQSLNSQAAGN
                     SDGAGATNLAAREQTAREAGRKAEIGTVNDLLGISVDHFVEVTLGAFFQIAKVVEPIT
                     VCLNADTVDSYSGADFHQGVQRIDAAQAMAFVRQRRDENDGSFTDFDRTRRQQAFLVS
                     LVKAVRDGGALSSLSAMRRLVQVARDNVAVDAGLDLLGFAARSSQLSDRPLSLYTLPI
                     SGFSKDPNGSDVNLVDVPTIRRIVHERFFSDTPAAVDAGPSSTPPPALSKPVVLDVVN
                     AASRDGLAAAIEDAFSDRGFTRGSASTAPAPADDSIVEYGPGADEGARMLADQLHVPA
                     TARDTVSPGTIRLTVGTRFPASDYLAHPGPTADGSASGQVTAVAATGSGDRAPAPTDL
                     SQMTATSIPCVK"
     gene            17815..19245
                     /locus_tag="VFI55_06765"
     CDS             17815..19245
                     /locus_tag="VFI55_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7073767.1"
                     /translation="MTTESVAPKTPASKNGASATQPVDVAATVARLRKTFATGRTRDL
                     AWRKHQLLQLQRLMEENEAAINAALAQDLDRSPFEAYIADIATTAGEAKYAAKRLRIW
                     TRRRYRLLEPSQLPGRGWVEYEPYGTVLIIGAWNYPFYLTLGPAVGAIAAGNAVVIKP
                     SEIAPASAHLMAELVPRYLDNDAIALVEGDGVVSQALIAQGLDRVMFTGGTEIGRKVY
                     EGAAPHLTPVTLELGGKSPVIVAADADVDVAAKRIAWLKLLNAGQTCVAPDYVLADVK
                     IRDELVSKIGAAITKFRSNDADGMRIVNQRQFDRLSGYLSGDDGKVTVGGKCDASSLR
                     IQPTVVVDPDPDGPLMRNEIFGPILPVVTVQSLDDAIRFVNSRPKPLSAYLFTKSRDV
                     RERVIKEVPAGGMLINHLAFQVSTAKLPFGGVGASGMGAYHGRYGFEEFSHRKSVLTK
                     PTRPDVSSFVYPPYTARAFKLARRLF"
     gene            19414..20274
                     /locus_tag="VFI55_06770"
     CDS             19414..20274
                     /locus_tag="VFI55_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7073768.1"
                     /translation="MPGVQDRVIAVTGAGGGLGREYALTLAREGACVIVNDLGGARDG
                     TGAGSAMADQVVAEIRDAGGRAAANYDSVAEPEGAENIIKTALDEFGAIHGVVSNAGI
                     LRDGTFHKMSFESWDAVLKVHLYGGYNVVRAAWPHFREQNYGRVVVATSTSGLFGNFG
                     QTNYGAAKLGLVGLINTLALEGAKYNIHANAVAPIAATRMTEDIMPPEVLEKLTPEFV
                     APVVAYLCTEESTDSGSVFVVGGGKAQRVALFGNDGVNFEQPPSVEDVAAHWAEITDL
                     SAAQKAGFKL"
     gene            20280..21248
                     /locus_tag="VFI55_06775"
     CDS             20280..21248
                     /locus_tag="VFI55_06775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HET7073769.1"
                     /translation="MKACVVQRLSGPRGMVYTDIDDVSGGANHVVIDVRAAGVCFPDL
                     LLTKGEYQLKLPPPFVPGMEAAGVVRSAPAGSGFRVGERVSAFGILGAYAEQVAVPVP
                     NVMRSPAELDDAEAVSLLVNYNTMYFALARRAAMRPGDTVLVLGAAGGVGTAAIQVAQ
                     AMGAKKVLAVVHREAATDFVAALGADMVVPLSDGWADRVREYTHGRGVDIVVDPIGGP
                     AFDDAIRVLAIDGKLLVIGFAAGSIPTVKVNRLLLRNVSVMGVAWGEYLNKVPGSAAL
                     FSWGLSQLVFLGLRPPPPQRYPLSEGQAALQSLADGGVLGKVVLEP"
     gene            complement(21252..>21453)
                     /locus_tag="VFI55_06780"
     CDS             complement(21252..>21453)
                     /locus_tag="VFI55_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073770.1"
                     /translation="EQVIDGHPVDGLIYAFGAPLAADTALATLFGGFEVRVFQHAAST
                     IVGAFTDSAYTPPPQQTQTVTL"
BASE COUNT         3667 a         7087 c         7212 g         3487 t
ORIGIN      
        1 ccgacgagga cgtgcagatc tgcgtgtccg tcccagtcgg cgacgtcgac ttgtcgctct
       61 aacaaccctt tgagagggca aagccatgtc aaagaacacg atcaccctga cccccgagca
      121 ggccgacgcg ttcggtcgcg agctcgacgc catcaaggaa cgcgtcacgg ccgacctcgg
      181 cgaaaaagac gccgagtaca tccgccgcgt catcaaaacc caacgcgcat tggaactcag
      241 cggccgcgcg ctgctgttcg ccgggttcct gcccccggcg tggctgctgg ggaccgcgat
      301 gctgggtgtg tcgaaaatcc tggacaacat ggagatcggt cacaacatca tgcacggcca
      361 gtacgactgg atgcgcgacc cgacgatctc cggccgttcc ttcgagtggg acacggcgtg
      421 ccccgccgat caatggcggc attcgcacaa ttacatgcac cacacccaca ccaacatcgt
      481 gggaatggac cgcgacatcg gctacggcat cctgcgaatg agcgaggacc agccctggga
      541 gccgtacttc ctgggcaacc cggtctacgc gttcctgctg atggtgttgt tccaatacgg
      601 cgttgcgctg cacgagatgg aaaccgagcg cattcgctcc ggagagatcc ggctgcgcga
      661 caagcgtgag gtgctgcggg caatctggaa gaagacgcgc cggcagacgc tcaaggacta
      721 tgtggcgttc ccgctgctgg ccggaccgtt cgccccgttc gtgctcgcgg gcaacctcac
      781 ggccaacctg atgcgcaacg tgtggtcgta catgatcatc ttctgcggcc atttcccgga
      841 cgggacacag gaattcaccg tcgaggagac caaggacgag tcgcgcggca tgtggtactt
      901 ccgccaggtc ctcggctcgg cgaacctcac cgggggcaag ctctttcatc tgatgtccgg
      961 caacctgtcg caccagatcg agcaccacct gttcccggac ttgccggcgc gccggtacgg
     1021 cgagatcgcg cccgaggtgc aagagatttg cgaacgctac ggcatcccgt acaaccgggg
     1081 cccgctgccg cgacagttcg ccaccgtggt gcgcaagatc gtcaggctgt ccttcccgga
     1141 ctcatggctg cggaagtccg aggtcggcaa gccggccgat cgggttccgg tcgccgcgtg
     1201 agcctgcgct gacggcgccg agcctgcgtg tagggtgcga tttttcgact ttgggtagca
     1261 ctgaacgcag gctcgatgga ttccgggtat gccgaccgca tgggagattt ccaggtcgag
     1321 ctggtcgagg gcgcgggcca ccggatcgtc gagcaacgac ccgagctggt actcgaccgg
     1381 atccggacgt tcctcagggc ttgaccagga tgcggatggg gttgccgtct tggcgctcca
     1441 gtttctcgat gcccagcgaa atgtcttcca gcgccacgat ctcgctgatc gagcgtgaaa
     1501 gatcaaggcg cccagccgaa accagcttcg ccagggtttc gatgtcgacg ttctgatagc
     1561 cgaggtggcc gagcacctgc ttacgggtca acccgaacat ggacgtgggc ccgatggtcg
     1621 gcgcctccgc gctcatcccg acggccacca gtcggcccgc ggaggtcaga cagcccagtg
     1681 cctgctcgaa cgtcgccttc aggccgacgg cgtcgaaggc cacatccagc agtctgccgc
     1741 cggtgatctc ggcgatcttg tcccggagcg cgtcgtcgcg ggagtcgaac gaatagtccg
     1801 cgccgaccgc cagggcccgg tccatcaccg ccgggttgat gtcgatcgcg attaccggca
     1861 cggcgcccac caaccgtgcg agctgcacga tgtgggtgcc gataccgccg acaccccaca
     1921 ccgcgaccga ttcgccgacc gacaccttgc ccgttcggac gacggctcca tagggcgtcg
     1981 acaccgcatc ggccaggatc gcggcctgct ccaacgggac gttgtccggc acccgggtta
     2041 ggcccgccgc ctgcgccacg gtgtattcgg cccacgcgcc gtcgtaggcg aaggccatca
     2101 attggattcg caggcagttc atcacatcgc cacgacggca gttcgggcat ttctggcagg
     2161 gccgcccggc ggcaaccacc acccggtcgc cctcggccca gccggatacg tccgggccga
     2221 cttccgcgat gatgcccgac gcttcgtggc cctgcgtcac cacgggcgcc tgcgccggga
     2281 aggtcccgtt gatcaggctc aggtccgagt ggcagatccc gcagaaggcc accttgacca
     2341 gcacttcgcc gggccctggc tgcgggatcg gaacatcttc cagcgcaatc tttttggtgt
     2401 ccgcgtagaa acgctccgcg cgcatcgtcg gcgtcatgtc ggttactcga cgccgatggt
     2461 gacttcggtg gacttgatga acaccgtcgc cggctggccg accgtgaggc cgaggctggt
     2521 ggcggcgtcc ttggtgaccg aggacgtgac gatttggccg ccgccgtcga gtttgacttt
     2581 cacgatggcc attacgctgc cgagatcgac ctcggtgatc gttccggtga gctgattacg
     2641 ggtcgatagc cgcatcgcaa tcctccgtgc ctagggtccg caatcgagcc tagcccggcg
     2701 acgatgcagg ccggaacggc ctgaggagga gccgggcaat tgagtccagc ccggcgacga
     2761 tacaggccgg aacggcgtgg ggtgggactc attcccggcc cgggtctcgg ctcagtgtcg
     2821 ggggccgagc aaggcgatgg atgcgtccac ggccggcgcg acgatctctc gcgtgggtct
     2881 gccgtcttcg acggccagcg cggtcagttc acgcagcccg gccagcagga tcacggccag
     2941 cggcgcggtc aacggcggca ggtcagcgcg ccggaatccc gggctggcgc tgaggtcgat
     3001 cagcaggttg gacagcaact gcaacccgcg gcgctggacg ggtcgagcga cggcacccag
     3061 cgacggaagc tcacgaatcc agctcagggt gatggccggg cgggactcga tgtatccgac
     3121 ataggcctcg acggcctgac gaatctgttg atgccagtcg gcgtcgggat cgaccgccac
     3181 ctgaatgctc tcgccgagct tctcgatgtc ggcgcgcaac agctccagaa agcactgttc
     3241 cttgctggcg aactggtcgt agaaggtgcg cttggaggtg cgggcgtggc ggacgatgtc
     3301 ggcgaccgtg ctggcgcggt agccacgctc gccgatcgag gtggcgaggc catcgagcaa
     3361 ccgaagccgg aacggatcgt tggtggccgg agggctcccg agaaccgcgt cgtccgccgc
     3421 ggcggtcacc gctgagatca cggccggcac ccccttcggt ggatacggca ccttgtcagg
     3481 tttggtacca cagagtaccg taccgggaaa ggtctgcggt acacggacgt accaccccta
     3541 ctccggggca gatattggga gcatcaccgc catgagcgaa gtactcagtt ccccgccggc
     3601 gccccttgcc gtcaggttgc ccccggcgac ccgaatcccg aaggtcgtgt gcggcctgct
     3661 cttcgccacc tcgcggcgcg ggatgatcaa acgggtgacg cgccgttacg gcaacgtctt
     3721 cacactgaat gtccccattt acggccgggt cgtggtggtc ggtgacttgc agctggcgaa
     3781 acaggtcttc accaccagcc ccgacgaact cggcaacatc cagcccaatt tgagccggct
     3841 gttcggttcc ggctcggtgt tcgggctcga cggagacgat caccgccgcc ggcggcggtt
     3901 gctggcgccg ccgttccacg gcaagagcat gaagaactac gaggccatca tcgaagaaga
     3961 gaccctgcgc gagactgccg gctggcccga aggccggccg tttgccacgc tgccatcgat
     4021 gatgcgcatc acgctcaacg cgatcttgcg cgcaatcttt ggggccgacg gcgccgaact
     4081 cgacgagctg cgccgcctca tcccgccgtg ggtcacgctg ggctcgcggg tggccccgct
     4141 gcccaagccc aaacggaatt atggccgctt cagcccatgg gcccggctgg acgagtggcg
     4201 cgggcagtac gacgtcgtcc tcgacaagct gatcgcggcc gagcgggcgg atccgaatct
     4261 cgccgatcga accgatgtgc tcgcgctgat gctgcgcagc acctacgagg acggctcggc
     4321 catgtcgcgc aaggacattg gtgacgaact actcaccttg ctggcggccg gccacgaaac
     4381 gacggcggcc acgctggcgt gggcgttcga gcggctgagc cggcaccccg atgtactggc
     4441 ggccctggtc gaagaggccg ataacggcgc tcagggtggt gggaacgagc tgcgccaggc
     4501 gacgatcctg gaggtccagc gggccaggac ggtcatcgac ttcgccgctc gccgtgtgta
     4561 tcagccggct taccaactcg gcgaatgggt aattccccat ggggattcca tcatcgtcgg
     4621 catctcgcag atacacgacg accccgacgt cttcgcggac ccgggccgct tcgatccgca
     4681 acgctacatc ggaggcaagc cgtcggcctt cgcgtggatc cccttcggag gcggcacccg
     4741 gcggtgtgtg ggcgccgcgt tcgccaacat ggagatggac gtggtgctgc gaacggtgtt
     4801 gcgcaacttc accattgaga ccacgaccga tccgggggag cgatggcact gccggggggt
     4861 cgcgtacacc ccgaagaacg gcggacgggt tgtagtgcgc ccgcgctgaa gccggttatt
     4921 ggccggcggt tgcgcggcgc ggcagcttcc agccggggcg cacgtagtga caggtgtagc
     4981 cgttggggta gcgctccagg tagtcctggt gctcaggctc ggcctcccag aattcgccgg
     5041 ccgggctgac ctcggtcacc accttgcccg gccacaggcc cgacgcctcg acatcggcga
     5101 tggtgtccag cgcgattcgc ctctgctcgt cgtcgaggta gtagatcgcc gaccggtagc
     5161 tggtgcccct gtcgttgccc tgccggttct tggttgtcgg gtcgtggatc tggaagaaga
     5221 actccagcag cgcgcggtaa tcggtcaccg acgggtcgta gacgatttcg acggcctcgg
     5281 cgtgcgtgcc gtgattgcgg taggtcgcgt tgggggtgtc gccgccgctg tagcccaccc
     5341 gggtcgaaag cactccgggc tgcttgcgga tcaggtcttg cattccccaa aagcagccgc
     5401 ctgcaaggat ggctttctgg gtgttcgtca tgctcgtgtc ctcctgagta cacctggtcc
     5461 ccactgatgc ggtaactcaa ccgataacgc gctcgggagt cgatggcgtt ccccaggtta
     5521 cactccggcg atgacgtgta acggtgcgac ggaaccctcg tgagcacgcg agggttctcc
     5581 cgcagcgaac tgtccgccgc attcgagaag ttcgaggaaa cggtcgccag ggccgccaag
     5641 acgcgagact gggacgcctg ggtcgagcag tacacgccga acgtcgagta catcgagcac
     5701 gcggccggca ccatgcgagg tcgcgagcag gtgcgcgact ggattcagcg aacgatgacc
     5761 accttcccgg gcagccacat ggtcgcgttc ccgtcgttgt ggtcggtcat cgacgagccc
     5821 accggccggg tcatctgcga gctggacaac ccgatggccg atcccggcga cgggaccgtc
     5881 gtcagcgcga ccaacatctc catcatcacc tacgccggtg atggtcagtg gcgtcgtcaa
     5941 gaagatatct acaacccgtt gcgttttctg cgggcgggga tgaagtggtg ccgcaaggcg
     6001 caggccctgg gcacgctgga cgacgacgcc gcgcgctgga tgcgcgagaa cggcgggtag
     6061 ccgtgacgca agagggctcc gtgagcacca aacccaagct ggtcgtcggc gccaacggtt
     6121 ttctggggtc ccacgtgacc cgccggcttg tcgccgacgg cgcccaagtt cgcgccatgg
     6181 tgcgcccgac cgccaatacc cgtgccatcg acgacctttc gcccactcgg tgcacccgct
     6241 ttcatggcga cgtgttcgac accgccgcgt tgcgggccgc aatggacggt gtcgacgacg
     6301 tgtattactg cgtcgtcgac actcgcgcct ggttgcgcga cccgtcgccg ctgttccgca
     6361 ccaacgtcga gggcctgcgc aacgttctcg acgtcgccgt gcaacagtcc ggcctgcgca
     6421 ggttcgtctt caccagcacc tacgcgacgg tgggccggcg acacgggcac gtggcgacgg
     6481 aggaggacgt catcggctcg cgcgggctga ctccttatgt gcagtcgcgg gtgcaggccg
     6541 aagacttggt gatgcggtac gtggccgacg cggggttgcc cgccgtcgcg atgtgtgtct
     6601 ccacgaccta cggcagcggc gattggggcc gcaccacgca tggtgcgttc atcgccggcg
     6661 cggtgttcgg caagttgccg ttcacgatgg acggcatcca actggaagtc gtcggcgtcg
     6721 acgatgccgc ccgcgccatg atcctggccg ccgagcgtgg gcgcgtcggc gagcgttacc
     6781 tcatctccga acggatgatc gcgctgcgcg acgtcgtgcg gatcgcggcc gacgaggccg
     6841 gtgtgccgcc gccgcgacgc tcgatttcgg tgccgatgct gtacgcgctg ggtgcgctgg
     6901 gcagcctgaa ggcccggctc accggcaagg atgccgaact cagcctgaac tcggtgcgca
     6961 tgatgcgcgc agaagccgac gtcgacagca gcaaggcgaa gcgggaattg ggctggcagc
     7021 cgcgtccggt cgaggtatcc atccgcgagg cggcgcagtt ctgggccgca ctgaagggcg
     7081 cgcagaccgc ccgaagagac acgcagaacc gcaccgcaca ggccgaatag gctcggcccg
     7141 gtggaacgca ttccggtcga cctgcacggg ccgccgcaga ccatgctggc gacgctgtac
     7201 gccaaagcgc tcgacgccga cgcaccgcgg tcgattctga acgaccactt cgccaaagcc
     7261 gcggtggcgc agatcgatta cgactgggac aaaacgacga tcaccccgct gcgggcgccg
     7321 tcggtggcgg tccgcaccgc gcacttcgac aactggacgc gccagttcct ggccgtgcac
     7381 gagcaagccg tcgtgctgca tgtgggttgt ggcctggacg cccgggtgta tcgggtggat
     7441 cccggtcccg gggtgtggtg gtacgacgtc gactacccgg acgtcatcgg gctgcgcgag
     7501 cgggtgtatc cgggccgcgc gaattatcgg atgctgccgg cgtcggtgac ggatccgtcc
     7561 tggctggccg acatcgcggc cgacgggccc accctgttca tcggtgaggg cctgacgatg
     7621 tacctgagca aggacgacgg gttggcgctg ctgcgccgag tcgtcgaccg atttccctcc
     7681 ggcgaactgc aattcgatgc gttcaacacc ttcggtatcc gcacccagtg gatcaacgcc
     7741 gtggttcgcc ggtcggggtc gacgttgcag tggggaatcg acgggcccgg cgacatcgtc
     7801 gacgcggtag ccggagtgcg cctgttggtg tgggaatcgg tgttcgacag cgatacgttc
     7861 gacctggttt caccggtcgc gcgccaggtg ggcagggtga tggcggtggt ccccgtgttg
     7921 cggacgatgg cgcaatacca ccggtatgcg ttcgggcccg cttgatgccg cgtgacgccg
     7981 ggaagcgttc gacgccggcc ggcgttggaa ggggcatctg catctgagga ggaagcaatc
     8041 atggctcacc gacaagtcct agagcccagc gccgggcacc ccatcaccat cgagccgacc
     8101 aagggacggg tgcaggttcg cgtcaatggc gagctggtcg cggacacgac ggccgcgctg
     8161 gaattgcgcg aggcgacttt gcctgtggtg caatacattc cactgggcga cgtggtgcag
     8221 gaccggctga cccgcaccga caccagcacc tactgcccgt tcaagggcga cgccagttac
     8281 tacagcgtga ccaccgccga cggctacacc gtcgaagatg tgatctggac gtacgaacag
     8341 ccctacccag ccgttgcggc gatcgcgggc cacgtcgcgt tctacccgaa caaggccgaa
     8401 atcagcgtgg cctaatacac ccgagtgggc gactcgggca gcgcctcggc ctgcgatcgg
     8461 gtgtcgctgt attcgcgcat cgagatgatc agcccgtcgc gaatctcgaa gatgcaggcg
     8521 aatgggctgt cgtatgcgac tcccttggcg ctggtgccgg tggcatggcc ttcgacgact
     8581 accgaaccgc cctcgctgat gcagcgcagc aggtcgacgc cgagttcgag gtgctttttg
     8641 cgccagttca ccgcttgccg cagggtcttc ttatcgaacg agacccgggt gaaaaggctc
     8701 caataggtga agtcgtcgct gagtagcgcg aacccctcgt cgaggtcacc cccctcacac
     8761 aggctctgca gaaacatcca ggcaagttcg gtgtgcgggc catcgaaggg cgtcatcaca
     8821 tcgcaatatt gtcttgggac atagttcacg gtcaaactag gcggtcggag aaccgcctat
     8881 gacctaaccg gattgcaggt tatcggtgga gagcgcgcgc acggtcaggt tgcccggtca
     8941 gctcagcttc gggcatacgc tggcgccgct gcgccgcggt ccccgcgatc cgtgcttcca
     9001 ggctgccggt gcagaaggcg ctatctggcg gaccagcctg ctgcccaccg ggcccgtcac
     9061 agcgcggatc agccgcgcgg cacccgacgc ggcccattgc gtggcgtggg gttccggtgc
     9121 ccaggagttc atcgaaatgc tgcccgcgct gctgggcgcc gacgacgacg cctcggactt
     9181 cgtgccgcgc gacccgacgg ttgccgccgc gcaccgacgg gttccgcatc tgcgcctggg
     9241 ccgcaccgga ttggtgctcg aagccctcat tccggcgatc atcgagcagc gggtacccgg
     9301 cgccgacgcg tttcggtcgt ggcgtctcct ggtgtccagg aacgggacgc ctgcacccgg
     9361 acccgccccg gccggcatgc gggtaccgcc gtctgccgag gcatggcgaa acatcccgtc
     9421 gtgggaattt catcgcgcca acgtcgatcc ccgacgggct cagaccgtgg tgagttgcgc
     9481 gcggcgggca gcttcgctgg aacggctggt gacgaggacg accgcgcagg cgcgagaagc
     9541 attgatgtcc ttgcccggcg tcggcgagtg gactgccgcg gagaccgcgc aacgtgcgtt
     9601 cggtgacgcc gacgccgttt cggtgggcga ctatcacatc ccgaagatga tcggctggac
     9661 gttgctgggc cggccggtcg acgacgccgg catgctcgag ctgctggagc cgatgcggcc
     9721 gcaccgccac cgggtggtgc gtttgctcga agccagcggc gtggcatccg agccgcgtcg
     9781 gggtgctcgg ctgccggtgc agcaaattca ttcgctgtga cctgccggac aggccggtaa
     9841 gagcaccgaa aggttgccgc gagcaccgct cttcgcaagt cctagctttc gacggccggc
     9901 tcgccctcgg cggccgcgga tgtgtcacgt gtcagtcgct gcgtctcgta gatggtgacg
     9961 ttggtgcgcg acatcaccaa cgccgcgatc ccgacggaca ggatggcccc gggcaccacc
    10021 gagaacgaac ccgtcatctc cgcgaccatg atcatgatcg ccagtggtgc gcgggcgacg
    10081 ctgccgaagc acgtcatcat tcccaccacg acgaaaatcc ccggcgcatc gggtacaccg
    10141 ggcaggccgg acagttcacc cagccgccag accgcggctc ccacgaaggc gccgatcaca
    10201 attccgggcc cgaacagtcc acccgatccg ccggtgccga tcgacagtga cgtggcgacg
    10261 atcttggcga acggcaggac gatgacgatc cacaacggga tcgccgtgag cgatccccga
    10321 tcggcggcca gctgcgccca gccgtagccg ctgctcagga tctgcggaat cagcagtccc
    10381 agcagcccga ccagaagccc gcccaacgcc ggcttgagca ccggaccgcc gggcagccgc
    10441 tgggtgagcc gcaccgacgc gtggaatgtc cgggcgtaca ggtagccgat cgccgccgcg
    10501 accagaccga tcaccacaaa ccacaacagc ggccacgccc gttcgaagcg gtactcggcg
    10561 tcgatgtagc cgaacagcgg atcgaacccc aggaaagccc caagcaccgc atacgccgtg
    10621 ccggaggtga tgaaacccgg cagcaggcag cggtagtcga agtcgtcgcg gtacgggagc
    10681 gacgcggcaa gcaccgcccc gcccagcggc gcggcaaaga tcgcgccgat gccggcgccg
    10741 atgcccagcg ccactgcgat ccgcccgtcc tcgtcggaca ggttgagccg tcgggtgagc
    10801 agcgagctga agcctgccga tatctgtgcc gtcgggcctt cgcgccccgc cgagccgccc
    10861 gagcctatcg tcagcgcgct ggcgaccatc ttcaccagca ccacccgaaa gcggatcgcg
    10921 cggggatcgg cgtgcaccgc ctcgatggct tcgtcggtgc cgtggccgct ggcctccggg
    10981 gcgagcttcg ccacgatcag cgcggacagc agcgctccgg ccgtcgtcac caacgggatc
    11041 gcccacggac gctgaaagcc ggccgaacca tggctgccgc cctccccgac gggcgtcggg
    11101 atgtggtagc cggccaggta cccgagcaga aactcggacg tgtatttcag caacagatag
    11161 aagacgacgg ctccaaggcc cgcgacgcca ccgatcgtga tgcccagtaa cagccacttt
    11221 tgcaggtagc ccgacttcct gatcgacgcc ccgaagcgtc cgccggccgc gggagcggga
    11281 gcctccgggg gcccgggttc gtcatccacg tgttcgctca cggcatggac ctgacccgcg
    11341 tcggtcgctc cggtgcaccc ggcatcgcgc ctccttgcag cgaagggaat aactggactg
    11401 cggtccggtt atatgagtag ctctgaacaa aggaggaaaa tatgcctgct attaccgcag
    11461 acaccataac gttgccgcgg gtcggcccgc ccggccccgg cgacaccgaa cggccggtcc
    11521 ggtcgatcac caccggcccc cggggctacg agggcgaggg tttccccgtg gtccgcgcgt
    11581 ttgccggggt cagcgacgcc gctctggacc cattcgtaca tatggaccag atgggtgagg
    11641 tcgactacga gccgggcgag cccaggggca ccgattggca cccgcaccgc gggttcgaaa
    11701 ccgtcaccta catgatcgac ggcaagttcg cgcaccagga ctcccatggg ggcggcggcc
    11761 tgatcgccga cggcgcgacg caatggatga cggccggttc ggggatcctg cacatcgaga
    11821 ccccgcccgc cgaactggtc gaaagcggcg gcctcttcca cggcgtccag ctgtgggtga
    11881 acctgccgaa gaaagacaaa ttcaccgcac ctcggtacca ggccatcgaa ggcggcgacg
    11941 ccaaactgct cgcctccgac gacggaggag cgctggtccg catcatcgcc ggtgagatcg
    12001 acggtcaccg cggacccgga gtcacccaca cgccgatcac gttggcgcac gtcacgatcg
    12061 aaaacggcgg ccgcctcaac attccgtggc ggcgcgattt caacgcgttg gtgtacgtgt
    12121 tgtccgggag cggctacgtc ggtccggtcg gtcacccgat tcgctcacgt caattggccg
    12181 tcctcgggcc cggtgaccgg atcaccgtcg gcgccgatcg gacgcaggag tcgacgcgga
    12241 cgacgtcaat tgatgtgctg ctgctgggcg ggcagcccat tcgcgagccg gtattccact
    12301 acgggccttt cgtgatgaac acccgcgagg aattgatcga ggcgttggag gactaccaag
    12361 ctggaaaatt tggcaatatt ccgccgaacg cgttaatgcc acatcatggc ggtggcacac
    12421 tttaataatg ccgccgacgg caggtcgccg gccgggccgt ccgcccgccg cgaaggcaga
    12481 tgacacgcgc aagcgcatct tgcacgcggc ccgtcaggtg ttcagcgagc gtggttacga
    12541 cggagcgact ttccaggcga tcgcctcgcg agccgatctg accagaccgg cgatcaatca
    12601 ctacttttcg agcaagcggg cgctctaccg ggaggtggtg gcccaagcca acgagcttgt
    12661 tgtcgctgcc ggcatcgaac gtgccgcgtg cgaggtcacc ctgatggcgc gcctgacggc
    12721 gttcatctcc gacgcggtgc gtgcgaattc ggtaaatccg tcggcgtcgg catttctggt
    12781 tactaatgtg ctcgaatcgc agcggcatcc ggaactggtg gcagccgaaa atgacgccgt
    12841 gctaattacc cgcgaatttc tgacacgcgc cgtcaacgaa gcgattgaac ggggcgagat
    12901 cgtgaccgac atcgatgcgt ccgcattggt cgagacgctg ttggtcgtgc tctgcggcgt
    12961 ggggttgtac gccggctatg tccggaccta cgaagagatg ttggccgtga ccgacacgct
    13021 gcggcagctc ctcgaaggtg cgctctggcg atccgaggcc tagcacgggc acacaactcg
    13081 atccgtcgca gtgacgtcgc gcacaaggcg tatagcctaa ctaacttact cggtagcatg
    13141 gtcccgacat gactgatctg gatgcctcgt taccgccttc actacggacc gccggcgaca
    13201 gctgggagat caccgaactc gtcggcgcca ccgcgctggg cgtcgccgcg tcgcgtgcgg
    13261 tggaaacggc cggacctaac ccgctgatcc gcgacgactt cgcccaaatc ttggtgtcgt
    13321 cggccggccc cgcgtgggcg cggttgactg actccgagct ggcctggttg gacggcgacg
    13381 agcacggaca acgggtgcac cgcctgggca tcgcctatca ggcggtgcgc acccactttt
    13441 tcgacgagta cttcgccgac gccgtcggcg ccgggattcg acaggtggtg atcttggccg
    13501 ccggcctgga ctcccgtgcc taccggctga gttggccggc gggtaccgcg gtctatgaga
    13561 tcgaccagcc caaggtcctg gagtacaaga ccggaatttt ggaatcgcat ggcgcggttc
    13621 cgaccgccga ccggcgtccg gtcccggtgg acctgcgcca cgactggccg gcggcgctga
    13681 cggccgcggg attcgaccgc acccaaccga ccgcgtggct tgccgagggc ttgctgccct
    13741 acctgcccag cgacgcccag gaccggctct tcgagatgtt caccgcactc agtgccccgg
    13801 gtagccgggt cgccgtcgag gccttcggcc tgaactcgaa gggcaacacg cagcgttggc
    13861 tgcggatgcg ggagcgactc ggcctggatg tcaacgtcca ggcgttgacc taccacgaac
    13921 ccgaccgttc ggatgccgtc cagtggctgg ttgaccacgg ttggcaggtg cggcgcgtca
    13981 acaatcgcga ggagatggcg cgactgggcc ggccggtgcc cgacgacctg gacgacgagg
    14041 cggtgcgcag cacactgctg cgagcgcgtc tcggcgggcc ttccgattaa ccgagatccc
    14101 aacacgcaca gagcgggagc acgtcatgag cacaatgcgc acgcacgacg acacctggga
    14161 catcaagacc agcgtcggga gtaccgcggt gatggtggcc gctgcccggg ccgtcgagac
    14221 cgaacggccg gactcgttga tccgcgaccc ctacgcccga ttgctggtca ccaatgcggg
    14281 cgccaacgtg atgtgggagg cgatgctcga cccgtcgatg gtggccaagg tcgaggccat
    14341 cgacgccgag gccgcggcaa ccatccagca catgcgtagc taccaagcgg ttcggaccaa
    14401 cttcttcgac acctatatct tggccgctgc cgccgacggg atccgtcaga tcgtgatcct
    14461 ggcgtcgggg ctggattcac gcgcctaccg tctggactgg ccggccgaca ccgctgtgta
    14521 tgagatcgat cagccgaagg tgctggcgta caaatccacg acattggccg aaaacggagt
    14581 gaacccgggc gccgagcgcc gtgaggtacc catcgacctg cgtcaggact ggcccgccgc
    14641 gttgcgagcc gcgggttttg accccgctgc gcgaaccgcc tggctggccg aggggctgct
    14701 gatgtacctg ccggccgagg cacaggacaa gctgttcacc caggtcggcg agctcagcgc
    14761 ggcgggcagc cggatcgcgg ccgaaacctc gcccctgcac ggcgaggatc ggcggcaaca
    14821 aatgcgcgag cggttcaaga aggtgaccga cacgctcggc ttcgagcagg ccgttgacat
    14881 ccaggagctg atctaccacg acgaggaccg ggctgttctc gccgactggc tcaacggcca
    14941 cggttggcgg gccacggcgc agagtgcgtt ggacgagatg cggcggctgg gccgctcggt
    15001 tgacggcgtg cccatgggcg acgacaagga tgcgttcgcc gagtttgtca ccgcggagcg
    15061 gttgtagtgg cgcgcacggc cgatgactcg tgggacatcg cgaccagcgt cggcgccacc
    15121 gcggtgatgg ttgcgctggc ccgcgccgcc gagacggcca gcgccgaccc gctgatccgg
    15181 gaccgattcg ccgaactgct ggtgtctact cccgaactcg agggggttcg ggagcaggtg
    15241 gcggcctggt gggtccccga gccggatgac gactcggggg aggggaccga tccggatctc
    15301 gcggtcgatg cccagcagat gatcgattac caggcggtgc gcacccattt cttcgacgcg
    15361 tacttcgccg gggccgtcgc caccggtatc cggcaggtcg tgattctggc cgccggcctg
    15421 gattcccgcg cctaccggct ggactggccg gacgggaccg tcgtctacga gatcgacctg
    15481 cccaaggtgc tggagtacaa gaccgcgacg ctggccggtc agggcgccgc tccaatcgcc
    15541 gaccggcgac ccgtggcggt cgacctgcgc cacgattggc cccaggcgct gcgtgacgcc
    15601 gggttcgatg ccaaccagcc gacggcctgg ctcgccgaag ggctattgcc gtttctgccg
    15661 gccgccgctc aggagtcgat gttcgcctcg atcgaccggc tgagcggacc ggccagccgg
    15721 gtggccgtag aaatcttcgg ggtcgacgaa gacaagcgcc gcgaggccga agagaggtgg
    15781 cagcagctgc gggccaagcg cgccgcgcgc ggccacgaca cctcgttcaa ccccttcgac
    15841 ctctggttcg acgacgacgg acgtccggat tccgccgact ggttcacagc gcagggctgg
    15901 acgagccgga cggtgaacgc ccgcgaggag gcgctgcgac tgggacgtgc gccgcaatcc
    15961 gaggaccggc cgttcacgaa cagcttcgtg acggccacca aaccgtgaag acgcccgccc
    16021 tcgtgcgctg aacgtcgtcc ggcccgcggt aactccctat ttgcgaatgg ctcgcggttc
    16081 cggttaccgg ggattcgccg ggtggtcgac cacaatggtg agtcattatg agcagcagcg
    16141 aatctggtca accccagcgc ctggtggtgt tgaaagggcg gctgctggcc agcatggctt
    16201 cggctttcgt gatggccatt accgccatcg ggtggaccgg ctaccacacg gcagtgggca
    16261 agatcatcat ctcccacgta cttccgaatg cacagacgcc ggccggcgcc gatcagaaca
    16321 ttctgctcat gggcttggac agccgattgg atcagaatgg ccagccgctg ccgcaggaaa
    16381 tgtatgaggc attgcacgcg ggcgatgaga gctccggggg ctacaacgcg aatgtgctga
    16441 tagtcgtcca catttcgggt ggggacgggc cggtcaccgc ggtgtcgatt ccccgcgacg
    16501 actacgtgga tctgtcgggc tgtccaggat cggtgtgcaa aggcaaggtc aagcaagcct
    16561 acgggctggc ctatcagcag tccctgaatt cccaggcggc cggcaattcg gacggcgcgg
    16621 gcgcgaccaa tctggccgct cgcgagcaga ccgcccgcga ggccggccgc aaggcagaaa
    16681 tcggcaccgt caacgatttg ttgggcatct cggtcgacca cttcgtcgaa gtcacgctgg
    16741 gcgcgttctt ccagatcgcg aaggtcgtcg agccgatcac ggtgtgcctc aacgccgaca
    16801 ccgtcgacag ctattcgggt gcggacttcc accaaggggt gcagcggatc gatgccgcgc
    16861 aggcgatggc cttcgtgcgc cagcggcgtg acgagaacga cggctccttc actgacttcg
    16921 accggacgcg acgccagcag gcgttcctgg tgtccttggt caaggcggtc cgagacggcg
    16981 gtgccctgtc cagcctgagc gcaatgcgca gactggtgca ggtggcccgc gacaacgtcg
    17041 ccgtcgacgc cggtttggac cttctcggtt tcgcggcgcg gtcttcgcag ctgagcgacc
    17101 ggccgctgtc cctctacacc ctgccgatct ccggtttttc caaggatccc aacggatccg
    17161 acgtcaacct cgtcgacgtt ccgaccatcc ggcgaatcgt tcacgagcga ttcttctcgg
    17221 acacaccggc cgcggtcgat gccgggccgt cgagcacccc accgcccgcg ctgtccaagc
    17281 cggtcgttct tgacgtcgtc aacgccgcgt cgcgcgatgg gctggccgcc gcgatcgagg
    17341 acgcgttctc cgaccgcggt ttcacccggg gcagcgcgag cacggccccg gccccggccg
    17401 acgacagcat cgtcgaatac ggtcctggcg ccgacgaagg cgcgcggatg ttggccgatc
    17461 agctgcacgt gcccgcgacc gcgagggaca ccgtgtcacc cgggacgatc cggcttaccg
    17521 tgggaacgcg gtttccggcg agcgactacc tcgcccatcc gggccccacg gccgatgggt
    17581 cggcatccgg gcaggtgacc gcggtggccg ccacgggcag cggcgaccgc gctcccgcgc
    17641 cgaccgacct cagccagatg acggcgacca gcatcccctg cgtcaagtaa ccgccgcccg
    17701 ggtccttcct tactgcttct ctcgccggat ggctaggatc gcggttatac cgatgagcgg
    17761 gcgtccgcaa ctccagcggg caactccgct cgggaagaac tcgggaggac aacgatgacc
    17821 accgaatcgg ttgcaccgaa gacaccggca tccaagaacg gcgcgtcggc aactcagcca
    17881 gtggatgtcg cggcgacggt agcccggctt cgcaagacct tcgccaccgg ccgcacccgc
    17941 gacctcgcgt ggcgcaaaca tcagctgctg cagctgcaga ggctgatgga ggagaacgag
    18001 gccgcgatca acgccgcgct ggcgcaggat ctggatcgca gcccgttcga ggcatacatc
    18061 gccgacatcg ccaccaccgc cggcgaagcg aagtacgcgg ccaagcggct gcgcatttgg
    18121 acgcgccgca gataccggct gctcgagccg tcgcagctgc ccggccgcgg gtgggtggag
    18181 tacgaaccct acggaaccgt gctgatcatc ggcgcctgga attacccgtt ctacctgaca
    18241 ctgggtccag cagtcggggc gatcgccgcc ggcaacgccg tggtgatcaa gccgtcggaa
    18301 atcgcacccg cgtccgcgca tttgatggcc gaactggtgc ctcgctacct cgacaacgac
    18361 gcgatcgccc tggtcgaggg cgacggtgtg gtgagccagg cgctgatcgc ccagggcctg
    18421 gaccgcgtga tgttcaccgg cggcaccgag atcggccgca aggtctacga gggcgcggct
    18481 ccccacctca cccctgtgac cctcgaactc ggcggcaaga gcccggtgat cgtcgcggcc
    18541 gacgccgacg tggacgtcgc ggccaagcgg atcgcctggc tcaaactgct caacgccggg
    18601 cagacctgcg tcgcacccga ctatgtgctg gccgacgtga agatccggga tgaactcgtc
    18661 agcaagatcg gcgccgcgat cacgaaattc cggtccaacg acgccgacgg aatgcgcatc
    18721 gtcaatcagc gccagttcga ccggctcagc ggctacctgt ccggcgacga cggcaaggtc
    18781 accgtcggcg gcaagtgtga cgcatcgagc ctgcgcatcc aaccgacggt tgtcgtcgat
    18841 cccgacccgg acgggccgct gatgcgcaac gagatcttcg ggcccatcct gccggtggtc
    18901 accgtgcaat ccctggacga cgcaatacgt ttcgtcaact cgcggcccaa gcccctgtcc
    18961 gcttatctgt tcaccaagtc gcgcgacgtg cgcgagcggg tgatcaaaga ggtgccggcc
    19021 ggcggcatgc tgatcaacca cctggccttc caggtgtcga cggccaagct gccgttcggc
    19081 ggggtcggcg cctcgggcat gggcgcctac cacggccgct acggcttcga ggagttcagc
    19141 caccgcaagt cggtgctgac caaaccgacc cggcccgacg tctcgagctt tgtgtatccg
    19201 ccctacaccg cccgggcctt caagttggcc cgcagattgt tctgacctga gtgccgatgc
    19261 ccgcccggcc cccccagacc ggggtcccgc gctcgcgggc cgagccggac acccagacct
    19321 ggcaaaggcc cggctggtgg tggcgcccgc tccgggcacc gatagcctct agtcccgaat
    19381 aggccaaaat ccttaacagg agagaggaac ctgatgcctg gagtgcagga tcgtgtcatc
    19441 gccgtcaccg gggccggtgg ggggctgggc cgtgaatacg ccctgaccct cgctcgggag
    19501 ggcgcctgcg tcatcgtcaa cgacctcggt ggggcccgcg acggcacggg cgccggatcg
    19561 gcgatggccg accaggtggt tgcggagatc cgcgacgcgg gcggccgggc ggccgccaac
    19621 tatgacagcg tcgccgagcc cgagggcgcc gagaacatca tcaagaccgc actcgacgag
    19681 ttcggcgcga tacacggcgt ggtgagcaac gccgggatcc tgcgcgacgg caccttccac
    19741 aagatgtcgt tcgagagctg ggacgccgtg ctcaaggtgc acctctacgg cgggtacaac
    19801 gtggtccgcg ccgcgtggcc gcatttccgt gagcagaatt acggccgggt ggtcgtcgcc
    19861 acctcgacca gcgggctgtt cggcaacttc ggccagacca actacggggc cgccaagctc
    19921 ggcctggtcg gcctgatcaa cacgctggcg ctcgaaggcg cgaagtacaa catccacgcc
    19981 aacgcggttg cacccatcgc ggccaccagg atgaccgagg acatcatgcc gcccgaagtg
    20041 ctggagaagc tcacgccaga attcgtcgcg cccgtggtgg cctacctgtg caccgaggaa
    20101 tccacggaca gcggatcggt tttcgtggtc ggtggtggca aggcgcagcg tgtcgcgctg
    20161 ttcggaaacg acggcgtcaa cttcgagcag ccgccgtcgg tggaggacgt cgccgcgcac
    20221 tgggccgaga tcacggacct gtccgcagcg caaaaagctg gattcaagct gtaggagcca
    20281 tgaaagcctg tgtggtacag cggctgtccg gcccaagggg catggtctac acggacatcg
    20341 acgatgtcag cggcggcgcc aaccacgtcg tgatcgacgt ccgggccgcc ggggtttgtt
    20401 ttccggatct gctgctgacc aagggtgagt accaactgaa gttgccaccg ccgttcgtcc
    20461 ccggcatgga ggcggcgggc gtggtgcgtt cggcgccggc cggttcgggt ttccgcgtgg
    20521 gcgaacgtgt ttcggcgttc gggatcctgg gcgcgtacgc cgagcaggtg gccgtcccgg
    20581 tgccgaacgt gatgcgcagc cccgcggaac tcgatgacgc cgaagcggtt tcgctgctgg
    20641 tcaactacaa caccatgtac ttcgccctgg ctcgccgggc cgcgatgcgg cccggcgaca
    20701 cggtgctggt cctgggtgcg gccggcgggg tgggcacagc ggcgatccag gtcgcccagg
    20761 cgatgggtgc aaagaaggtg ctggccgtgg tgcaccgcga ggccgcgacc gattttgtcg
    20821 ccgcgctcgg cgcggacatg gtggtgccgc tgtccgacgg atgggccgac cgggtgcgtg
    20881 agtacaccca cggccggggc gtggacatcg tcgtcgatcc catcgggggc ccggccttcg
    20941 acgacgcgat tcgtgtcctg gcgatcgacg gcaagctgct cgtgatcggg ttcgccgcgg
    21001 gcagcatccc caccgtcaag gtcaaccggc tgctgctgcg caacgtcagc gtgatgggtg
    21061 tcgcctgggg cgagtacctc aacaaagtcc ccggttcggc ggcgctgttc tcctggggac
    21121 tgagtcaatt ggttttcttg gggctgagac cgcctccgcc gcaacgctat ccgctgtcgg
    21181 aaggccaggc ggcgttgcag agcctggccg acggcggggt gctcggcaag gtcgtgctcg
    21241 agccctaggt attagagggt caccgtctgg gtctgttggg gtggcggcgt gtacgccgaa
    21301 tctgtgaagg ctccgacaat cgttgaggcc gcgtgctgga agactcggac ttcgaacccg
    21361 ccgaagagcg tggccaatgc ggtgtcggcg gcaagcgggg cgccgaaggc gtagatcagg
    21421 ccgtcgaccg ggtggccgtc gatcacttgc tcc
//
LOCUS       DASSGQ010000149         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_101737,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000149 DASSGQ010000000
VERSION     DASSGQ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101737"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFI55_06785"
     CDS             <1..238
                     /locus_tag="VFI55_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073771.1"
                     /translation="PDRMLIGIDDLARETSFVAYDEATIDELYFLAQEHANREVGPGK
                     EAYNVKVGGMGTPLTGDESRKSWPSTYKVRDKRH"
     gene            324..1907
                     /locus_tag="VFI55_06790"
     CDS             324..1907
                     /locus_tag="VFI55_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HET7073772.1"
                     /translation="MARRSLATGTALLTVAACSSAPSPSLSGEPTKELSTGAGKVLTI
                     SPGQLLAATSGVTPVAFGKPPHGNVAYGAYGAMVYTPDPGFTGTDQLPVTVSHAVRLY
                     AEDEPPLIIIGEVAVQANAHGSAIAQVPGSADEIYGLADRGPNVDGRTPDEKVLPVPD
                     FHPQIAKLKLSDGVASLEQIITLSGKDGAPLVGLIDPRVTTGESLVDLNGNRLAPSDH
                     GLDSEGLVAMPDGTFWVSDEYGPFIVHFDPSGKELERLSPFDRTLPKELSLHGLNQGM
                     EGLTITPDGHTLVGVMQSALNTPGLVGSTESVPVTRIVTVNLANREDVHEYLYPLANP
                     QQTNIVVSEITAVSASTFLIDEHDNQPQPNGTKKIYLADTSGATDVGPHSTAPGASYR
                     ADAGGLLINGVPIEAFVGVSTDAAAVDKLKAAGITVTGKTLTLDLGGLVRSLSANGDF
                     FGHDKIEGVINRDGGKTLIIANDSDFGLAGLADDTPPFQLRPKMLPNGTQDSGEILSV
                     DMTALPATTESVTVPIKVG"
     gene            2025..>2608
                     /locus_tag="VFI55_06795"
     CDS             2025..>2608
                     /locus_tag="VFI55_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET7073773.1"
                     /translation="MNALSPDAIRAETITITGHGGDEIEAYRAVPLADGSRGGIVWIH
                     HMPGYDRETKEFARRLAVDGYHVVAPNLYSREAPGAAPDDAAAAARAAGGVPDERLVG
                     DVAGAVEHLRSLPGANGQFGVIGHCSGGRHAYLAACSLPFDAAVDCYGGYVVEEPPEG
                     RKIRPILNLAPNLSCPLLGLFGVEDTFPAPSAVAA"
BASE COUNT          464 a          910 c          834 g          400 t
ORIGIN      
        1 gcctgaccgg atgctcatcg gcatcgacga cctcgcccgg gagacctcat tcgtcgccta
       61 cgacgaagcc accatcgacg agctgtactt cctggcccag gaacacgcca atcgcgaggt
      121 cggccccggc aaagaggcct ataacgtcaa ggttggcggg atgggaacgc cgctgaccgg
      181 cgacgagtcc cgaaagtcgt ggccgtccac ctacaaagtg cgcgacaagc gccactagag
      241 cgcggaatat ctgctagatg gcacgcaccg ggatgacaac gtttcttttc acgttccgtg
      301 ctaagccttc cggcgcgtcg tgcatggcca gacgatcgct ggcgaccgga acggcgttgc
      361 tcacggtggc ggcctgctcg tcggcgccct cgccctcgct gtcgggggaa cctacgaagg
      421 agttgagcac gggcgccgga aaggtcttga ccatctcccc gggccagctg ctggccgcaa
      481 cgagcggcgt gacgccggtg gcgttcggca aacccccgca cggcaacgtc gcatacggcg
      541 catacggcgc catggtctac acccccgacc ccggcttcac cggcaccgat cagcttcccg
      601 tcaccgtcag ccacgccgtc cggctctacg ccgaggacga gccgccgttg atcatcatcg
      661 gcgaggtcgc cgtgcaggcc aacgcgcacg ggtctgcgat cgcgcaggtt cccggcagcg
      721 ccgacgaaat ctacggtctg gccgaccgcg gaccgaacgt cgacgggcgc accccggacg
      781 aaaaggtgct tccggtgccc gacttccacc cgcagatcgc caagctcaaa ctctccgacg
      841 gcgtggcgtc gctggagcag atcatcacgc tgagcggtaa ggacggcgcg ccgttggtcg
      901 ggctcatcga cccgcgagtg accaccggtg agtcactggt ggacctcaac ggcaatcggc
      961 tggcgccgtc ggatcacggc ctggacagcg aaggcttggt cgccatgccc gacggtacgt
     1021 tctgggtgtc cgacgaatac ggtcccttca tcgtccattt cgatcccagt ggcaaagagc
     1081 tggaacgcct ttcgccgttc gatcgcacgc tgcccaaaga gctctcgctg cacggcctga
     1141 atcagggcat ggaagggctg acgatcacgc ccgacggcca caccctggtg ggcgtcatgc
     1201 agtcggccct caacaccccg ggcctggtcg gctcgaccga atccgtaccg gtcactcgga
     1261 tcgtgaccgt caacctggcc aaccgcgagg acgtgcacga gtacctctac cccttggcca
     1321 atccgcagca gacgaacatc gtggtctcgg agatcaccgc ggtgagtgcc agcacattcc
     1381 tgatcgacga gcatgacaac cagccgcagc ccaacggcac caagaagatc tatctggccg
     1441 acacctcggg ggccaccgac gtcgggcccc actcgacggc gccgggggcg tcctatcgcg
     1501 ccgacgccgg tggcctgctg atcaacggcg tccccatcga ggcgtttgtc ggcgtcagca
     1561 ccgacgcggc ggccgtcgac aagctcaaag ccgccgggat cacggtcacc gggaagaccc
     1621 tcacgcttga cctcggaggt ctggtgcgct cgctgtcggc caacggtgat ttcttcgggc
     1681 acgacaagat cgaaggcgtc atcaaccgcg acggcggaaa aacgctgatc atcgccaatg
     1741 acagcgattt cgggctggcc gggttagctg acgacacccc gccgtttcaa ctccggccca
     1801 aaatgctgcc caacggcact caagacagcg gcgagatcct ctccgtcgac atgaccgcgt
     1861 tgccggccac caccgaatcg gtgacggtgc cgatcaaggt cggctgacct gcatcctcgc
     1921 ggcaccatgc cgcggttcgg atacgttggg ttccggcggg gcgcaccccg acaagcgctc
     1981 cgcactccct tgcggccaga caaggagcct tagaaggagt tgcgatgaac gcgctcagtc
     2041 cagacgccat ccgtgccgaa accatcacga tcaccggcca cggcggcgac gagatcgagg
     2101 cctaccgggc tgttccactc gccgacggct cgcgcggtgg aatcgtgtgg atccaccaca
     2161 tgcccggcta cgaccgcgag accaaggagt tcgcccgccg gctcgccgtc gacggctacc
     2221 acgtggtcgc ccctaacctc tattcacgtg aggcgccggg tgccgcgccc gacgacgccg
     2281 ccgcggcggc acgggccgcc ggtggggtgc ccgacgagcg actggtcggc gatgtcgccg
     2341 gcgcggtgga gcacctgcgt tcgttgcccg gcgccaacgg gcaattcggc gtcatcgggc
     2401 actgctcggg tgggcggcac gcctacctgg cggcgtgctc gctgccgttc gacgcggcgg
     2461 tggactgcta cggcggctac gtcgtcgagg agccgccgga gggcaggaag atcaggccga
     2521 tattgaacct ggcgcccaac ctgagctgcc cgctgctggg cctgttcggg gtcgaggaca
     2581 cattcccggc gccgtctgcg gtggccgc
//
LOCUS       DASSGQ010000150         6799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_223078,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000150 DASSGQ010000000
VERSION     DASSGQ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6799
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223078"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1548
                     /locus_tag="VFI55_06800"
     CDS             <1..1548
                     /locus_tag="VFI55_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HET7073774.1"
                     /translation="GLERPVWPMIVLRTPKGWTGPKVVDGKKVEGTWRAHQVPLAETH
                     GNPAHRAQLEEWLRSYRPEELFDDDGRLRPELRAMAPRGNRRMSANPHTNGGMLLHDL
                     DLPDFREYAVPVPQPATSTHEATRILGTFLRDVIARNPDRFRLMGPDETASNRLDAVY
                     GATEKVWLSDVEPDDENLAPDGRVMEVLSEHLCQGWLEGYLLTGRHGLFNCYEAFVHI
                     VDSMMNQHAKWLATSRELPWRRPIASLNYLLTSHVWRQDHNGASHQDPGFIDLVANKR
                     AELTRVYLPPDGNTLLSVADHCLRSRGYINVIVAGKQPALAYLDMDEAVAHCTRGLGI
                     WQWASTATGEPDVVLACAGDIPTLETLAAADILRRELPDLAVRLVNVVDLMRLQPDSE
                     HPHGLPDREFDALFTRDKPVIFAYHGYPWLIHRLTYSRTNHQHLHVRGFKERGTTTTP
                     FDMAMLNDLDRFHLVMDVIDRVGGLASRAAVLRQRMVDARIAARSYTREHGEDDPQIA
                     GWVWGVE"
     gene            1713..2474
                     /locus_tag="VFI55_06805"
     CDS             1713..2474
                     /locus_tag="VFI55_06805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET7073775.1"
                     /translation="MLDATVAAHSHPVVSQLSALHRFRIHLDIGVIIVVLVLTNLIAH
                     FTTPWASIATVPAAAVGLAILMRVNGLDWADLGLGREHWKSGLAYALAAVAVVASVIA
                     IGVLLPMTRPMFMNNHYATISGALVASMVIIPLQTVIPEELAFRGVLHGALNRAWGFR
                     GVALAGSLLFGLWHIATSLGLTSNNVGFTRLFGGGVVGMLAGVIMAVLATGTAGFVFS
                     WLRRRSGSLIAPIALHWSLNGLGALAAALVWHLST"
     gene            complement(2489..3517)
                     /locus_tag="VFI55_06810"
     CDS             complement(2489..3517)
                     /locus_tag="VFI55_06810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HET7073776.1"
                     /translation="MPPTVTSTTMRAWRVRRPGPMATGPLEQVTTEVPCPEASELLVA
                     VQACGVCRTDLHVTEGDLPVHRDRVTPGHEVVGQVIEVGAEAGDDFKVGDRVGIAWLR
                     HTCGVCKYCRRGDENLCPQSRYTGWDADGGYAEFATVPAAFAHHLPGGYSNNELAPLL
                     CAGIIGYRSLLRAELPPGGRLGLYGFGGSAHITAQVALAQGAEVHVMTRGPQARELAL
                     ELGVASAQGAADPPPAPLDAAILFAPVGELVLPALDALDRGGTLAIAGIHLSDIPALN
                     YQRHLFQERQIRSVTSNTRADARALLDFAGRHHIKVTTPEYPLGQADQALADLSAGRI
                     AGAAVLLV"
     gene            complement(3556..3858)
                     /locus_tag="VFI55_06815"
     CDS             complement(3556..3858)
                     /locus_tag="VFI55_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET7073777.1"
                     /translation="MDVMAATEYLARSTTLTSVGVIGYIIIGGLAGWIGSKIVKGSGS
                     GVLMDIVIGVVGALIGGFILSFFVNTAGGGLIFTFFTALLGSVILLWLVGLVRSRR"
     gene            complement(4040..5032)
                     /locus_tag="VFI55_06820"
     CDS             complement(4040..5032)
                     /locus_tag="VFI55_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine and proline-rich secreted protein Apa"
                     /protein_id="HET7073778.1"
                     /translation="MDQVDPNSTRRNGLWAPLALAAVTSAFAVTIALPATSHADPEPA
                     PPPPPPTTVVAPPPGAPGLPPAPPGDPNAPPPPPVDPNAPPPPPVDPNAGRVGNPVGG
                     FSFVIPGGWVESDASHLDYGSELLSKTTGQPPMPGQPPPVANDTRVVMGRLDQKLYAS
                     AEANNAKAAVRLGSDMGEFFMPYPGTRINQEATPLTAANGITGSASYYEVKFSDPSKP
                     NGQIWTGVVGSPSTNGGPPQRWFVVWLGTSNDPVDKGAAKALAESIQPWAPPPNPAPE
                     GAPPPPGAPPPPPPPGAPAPAPPPAPPAPPGAPAPAPPPAAPPPPPAPGQAPAG"
     gene            complement(5141..6247)
                     /locus_tag="VFI55_06825"
     CDS             complement(5141..6247)
                     /locus_tag="VFI55_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HET7073779.1"
                     /translation="MTELQLRAVVAERDLDVEFSVSAGEVLAVLGPNGAGKSTALHVI
                     AGLVRPDEGMVRLGDRVLTDTAAGVDVPTHDRRVGLLLQDPLLFPHLSVAANVAFGPH
                     SRRAMFRSARSHEKALALRWLREVDVEDFAARKPRQLSGGQAQRVAIARALAAEPDVL
                     LLDEPLTGLDVAAAAGIRAVLRNVVSRTGRAVIMITHDLRDVFTLADRALVLESGRIS
                     EVGPIADVLTAPRSHFGARIAGINLVNGKVGSDGSLHARSGAHWHAIPAPGPSDQLAP
                     GHNAVAVFPPTAVAVYREHPHGSPRNAVEVIVAELDIRGSAVLVRGHEQSDGAPGLAA
                     EITVDAASELRLTPGERVWFSVKAHEVALYPAPR"
     gene            complement(6254..>6799)
                     /locus_tag="VFI55_06830"
     CDS             complement(6254..>6799)
                     /locus_tag="VFI55_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020725271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7073780.1"
                     /translation="RPLILLPLVLPPVVGGIALLYAFGRLGLIGRYLEAAGISIAFST
                     TAVVLAQTFVSLPFLVISIEGAARTAGADYEVVAATLGARPSTVWWRVTLPLLLPGMT
                     SGAVLAFARSLGEFGATLTFAGSRQGVTRTLPLEIYLQRVGDPDAAVALSILLVAVAA
                     VVVLGLGARRLTGNLTGNLTR"
BASE COUNT         1087 a         2313 c         2359 g         1040 t
ORIGIN      
        1 gggctggagc gacccgtgtg gccgatgatc gtgttgcgca ccccgaaggg ctggaccggg
       61 ccgaaggtgg tcgacggcaa gaaggtggag ggcacctggc gagcgcacca ggtgccgctg
      121 gccgaaaccc acggcaatcc cgcgcaccgc gcccagctgg aggaatggct gcgcagctac
      181 cgacccgagg agttgttcga cgacgacggc cgcctacgcc ccgaactgcg ggcgatggcg
      241 ccccggggta atcgccggat gagcgccaac ccgcacacca acggcgggat gctgctgcac
      301 gacctcgacc tgccagactt ccgcgagtac gccgtgccgg tgccgcaacc ggccacctcg
      361 acgcacgagg ccacccggat actgggcacc ttcctgcgcg atgtgattgc ccgcaacccg
      421 gaccgattcc gtctcatggg ccccgatgag accgcctcca accggctgga cgccgtgtat
      481 ggcgcgaccg aaaaggtgtg gctttccgac gtcgagcccg acgacgagaa cctcgcgccc
      541 gacggccggg tcatggaggt gctctccgag cacctctgcc agggctggct ggagggctac
      601 ctgctgaccg gacggcacgg cctgttcaac tgctacgaag cgttcgtgca catcgtcgac
      661 tcgatgatga accagcacgc gaaatggctt gccaccagcc gggagctgcc gtggcggcgg
      721 ccgatcgcgt cactgaacta cctgctcacc tcccacgtgt ggcgccagga ccacaacggc
      781 gcgtcgcatc aggacccggg tttcatcgac ctggtcgcga acaaacgggc cgagttgacg
      841 cgggtctacc tgccgccgga cggcaacacg ctgttgtcgg tggcggacca ctgcctgcgc
      901 agccgcggct acatcaacgt catcgtcgcc ggcaagcagc cggcgctggc ctacctggac
      961 atggacgagg cggtcgcgca ctgcacccgc ggcctgggga tctggcagtg ggcgagcacg
     1021 gccaccggcg aacccgatgt ggtgctggcc tgcgccgggg acatcccgac gctggagacg
     1081 ctggcggcgg ctgacatcct ccgccgcgaa ctgccggacc tcgctgtgcg gctggtcaac
     1141 gtcgtcgacc tcatgcgtct gcagccggat tccgagcatc cacatggctt gccggaccgg
     1201 gagttcgacg cgttgttcac ccgggacaag ccggtcatct tcgcctacca cgggtacccg
     1261 tggctgattc accggctcac ctattcgcgc accaatcacc agcaccttca tgtgcgcggc
     1321 ttcaaggagc gcggcaccac cacgacgccg ttcgacatgg cgatgctcaa cgacctggac
     1381 cgcttccacc tggtcatgga cgtcatcgac cgggtcggcg gcctggccag ccgagccgct
     1441 gtgctgcggc agcgcatggt cgacgcccgc atcgctgccc gcagctacac ccgcgaacac
     1501 ggcgaggacg acccgcagat cgccggttgg gtctggggag tcgagtgagc ggaacgaagt
     1561 gacgggaacg agacgaccaa tcgggctggc cggcgagcga gcggtagttc gacgccgaga
     1621 ttgccgtaat ggtcgtgctg cggtcggtga ccgcgaccat ggcggcaatc tcgcagaacc
     1681 gtgtcggcga ggcgctcggg taaactggcc ggatgcttga cgcgacggtt gctgcccatt
     1741 cccatccggt ggtgtcgcag ctttcggcgc tgcatcggtt ccggatccat ctcgacatcg
     1801 gcgtgatcat cgtggtgctg gtgctcacca acctgatcgc gcacttcacc accccgtggg
     1861 cgagcatcgc gaccgttccc gccgccgccg tcggcctggc gattttgatg cgcgtcaacg
     1921 gtctggactg ggccgatctc ggcttgggtc gcgaacactg gaagtccggc ttggcttatg
     1981 cgctggcggc cgtggccgtc gtggcgtccg tgatcgcgat cggtgtgctg ctaccgatga
     2041 cccggccgat gttcatgaac aaccactacg cgacgatctc cggcgccctg gtggcctcga
     2101 tggtcatcat tccgctgcaa accgtcatcc ccgaggagct ggcttttcgg ggtgtgctgc
     2161 acggcgcgct gaaccgggcg tggggctttc gcggggtagc gctggcggga tcgttgctgt
     2221 tcggtctctg gcatatcgcc acgtcgctgg ggctgaccag caacaacgtc ggcttcaccc
     2281 ggctcttcgg cggcggcgtc gtcgggatgc tcgccggggt gatcatggcg gtgttggcca
     2341 ccggtacggc cgggttcgtc ttcagctggc tgcgccggcg cagcggcagc ctgattgcgc
     2401 cgattgcgct gcactggtcg ctaaatgggc tcggtgccct ggcggcggcg ctggtctggc
     2461 acctgtccac ctagccccgg cggaccggtc aaaccagcag cacggctgcg ccggcgatgc
     2521 ggcccgcact gagatccgcc agcgcctggt cggcttgtcc gagcgggtac tccggcgtgg
     2581 tcaccttgat gtgatgccgg cccgcgaagt cgagaagagc ccgcgcgtcg gcccgcgtat
     2641 tggacgtcac cgaccggatc tggcgttcct ggaacaggtg acgctggtag ttgagggccg
     2701 ggatgtcgga caggtgaatc cctgcgatgg ctaacgtgcc accacggtcc aacgcatcga
     2761 gagcaggcag caccagctca cccaccggcg cgaacagaat cgcggcatca agcggcgccg
     2821 gtggcgggtc ggccgcgcct tgagccgatg cgacgccgag ttccagcgcc agctcgcgcg
     2881 cctgcggccc gcgggtcatc acgtggacct cggcgccttg tgccaaggcg acctgggcgg
     2941 tgatgtgggc gctgccgccg aagccgtaca ggcccagccg tccacccggt ggtagctcgg
     3001 cgcgcagcag ggagcggtat ccgatgatgc cggcgcacag caacggcgcg agctcgttgt
     3061 tgctgtaacc gcccggcagg tggtgcgcga aagcggcagg cacagtcgcg aattcggcgt
     3121 acccaccgtc ggcgtcccag ccggtgtagc gggactgcgg acacagattc tcatcgcccc
     3181 gccggcagta cttgcacaca ccacaggtgt ggcgcagcca ggcgatgccc acccggtccc
     3241 ccaccttgaa gtcgtcaccg gcctcggcac ccacctcgat gacctgcccc accacctcgt
     3301 ggccgggagt cacccggtca cggtgcacgg ggaggtcgcc ttccgtgacg tgcaggtcgg
     3361 tgcggcagac cccgcacgcc tggacggcaa ccagcaattc tgaggcctcg gggcacggga
     3421 cctctgtggt gacctgttcg aggggaccgg ttgccatcgg tccgggccga cgcacccgcc
     3481 acgcgcgcat tgtcgtagag gtcaccgtgg ggggcatcag ctcattttgc cgctacctgc
     3541 cacggcagca acggtttagc gccgacttcg gaccagcccg acgagccaga gcaggatcac
     3601 cgagccgagc agcgcggtga agaacgtgaa gatcagtcca ccaccagcgg tgttgacaaa
     3661 gaagctcaag ataaagccgc cgatcaacgc accgacgacc ccgatcacga tgtccatcag
     3721 gacgccagat ccgctgccct tgacgatctt gctgccgatc cacccggcga gaccgccgat
     3781 gatgatgtag ccaatcacgc cgacgctggt cagcgtggta gagcgggcga gatattcggt
     3841 tgctgccatg acgtccatag gggtctcctt gcaatcgccg gcaaagccca cctcgggcct
     3901 gatttcggta taccccgttt ggatgacgaa acgggtgccg aatcggccac gatgggcata
     3961 accgggctca accagcgaag agcgccgcca ttcaggccgg cacagtacgc tccggcgcgg
     4021 gtgtcgaagt cggagtcact tatcccgcgg gtgcctggcc cggtgccgga gggggaggcg
     4081 gcgcagccgg cggcggggcg ggcgctggcg caccgggcgg cgcaggtgga gcgggcggcg
     4141 gggcgggcgc tggcgcgccc ggtggcggag gcggcggtgg cgcgcccggt ggcggtggag
     4201 cgccttccgg agccgggttc ggcggggggg cccagggttg gatcgactcg gccagcgcct
     4261 tggcggcgcc tttgtccacg gggtcgttcg aggtcccgag ccacaccaca aaccagcgtt
     4321 gtggcggtcc gccgttcgtc gagggcgagc cgactacacc cgtccagatc tggccgttgg
     4381 gcttgctggg atcgctgaac ttgacctcgt agtacgacgc gcttccggtg atcccattgg
     4441 cggcggtcag cggggtcgct tcctggttga tccgggtgcc gggatacggc atgaagaact
     4501 cacccatgtc cgagcccagc cggaccgcgg ccttcgcgtt gttggcttcg gcgctggcgt
     4561 aaagcttttg gtccagccgg cccatcacca cgcgggtgtc attggcgacc ggcggcggct
     4621 gcccgggcat cggcggctgc ccggtggtct tgctgagcag ctccgagccg tagtcgaggt
     4681 gggacgcgtc cgactccacc cagccgcccg gaatgacaaa gctgaatcct ccgaccggat
     4741 taccgacccg gccggcgttc gggtcgacgg gcggtggcgg tggtgcgttc gggtcgaccg
     4801 gcggtggcgg tggtgcgttc gggtcgccgg gcggggccgg cggcaagccc ggggcgccgg
     4861 gaggcggtgc caccacggtg gtcggcggcg gcggcggtgg tgcgggctcc ggatcggcgt
     4921 gcgacgtggc cggcaacgcg atggtgacgg caaaagcact ggtcaccgcg gcgagggcaa
     4981 gcggcgccca caatccgttg cggcgtgtcg agttcgggtc cacctgatcc atggcgacga
     5041 acctaccgcg ttacggctgt tacgcaaggg tgcctggctg acgacgcgac gtggtttctc
     5101 cgatgttgcc gatatgcaac cctcgtcgct ttgacgcagg tcaacgcggc gccgggtaga
     5161 gcgccacctc gtgggccttg acggagaacc acacgcgctc ccccggcgtc agccgcagct
     5221 ccgaggcggc atcaacggtg atctccgcgg ccagaccggg tgcgccgtcg gattgctcgt
     5281 ggccacgcac caggaccgca gacccccgga tgtccagctc ggcgacgatg acctcaaccg
     5341 cgttgcgcgg gctgccgtgc gggtgctctc gatacacggc taccgctgtc ggtgggaata
     5401 ccgcgaccgc gttgtggccg ggcgcaagct gatcgcttgg tccgggggcg gggatcgcgt
     5461 gccagtgagc gccggagcgg gcatgcaacg aaccgtcgga acccaccttc ccgttgacca
     5521 ggttgatgcc cgcgatgcgc gccccgaaat gactacgtgg ggcagtgagc acatcggcaa
     5581 tcgggccgac ctcggagatc cttcccgatt cgagcaccag cgcccgatcg gccagcgtga
     5641 acacgtcccg caggtcgtgg gtgatcatga ttacggcgcg accggtccgg ctgaccacgt
     5701 tgcgcagcac cgcgcggatg cccgctgcgg cggcgacgtc gagcccggtg agtggctcgt
     5761 cgagcagcag cacgtcgggc tcggccgcca aggcgcgcgc gatcgctacc cgctgggctt
     5821 gacccccgga cagctgccgc ggttttcggg cggcgaagtc ttcgacgtcc acctcgcgca
     5881 gccagcgcag cgccagcgct ttctcgtggg agcgagccga gcggaacatt gcccgacggc
     5941 tgtgcggtcc gaaagccacg ttggcggcaa cgctcaggtg cgggaacagc aacgggtcct
     6001 gtaggaggag ccccacccgg cggtcgtgcg tcggcacgtc caccccggcc gcggtatcgg
     6061 tcaacacccg atcccccagc cgcaccatcc cttcgtcggg gcgaaccagg ccggcgatga
     6121 cgtgcagggc ggtcgacttg cccgcaccgt tgggcccgag cacggcaagc acctcgcccg
     6181 cagacaccga gaactccaca tccagatctc gttctgcgac gacggcgcga agctgcaact
     6241 cggtcatggc cggctacctg gtcagattcc cggtcaggtt cccggtcagt cgccgagcgc
     6301 caagacccag caccaccacc gccgccaccg ccaccagcag gatcgacaac gccacggccg
     6361 cgtccgggtc gccaacccgt tgcaggtaga tctccagcgg cagggtccgc gtgacccctt
     6421 gccgggaacc ggcgaaggtc agcgtcgcgc cgaactcccc cagggagcgg gcgaacgcca
     6481 gcaccgctcc cgacgtcatg cccggcagca acagcggcag cgtcacccgc caccacaccg
     6541 tgctgggtcg cgccccgagc gtcgcggcta ccacctcgta gtcagccccc gcggtgcggg
     6601 cggcgccctc gatggaaatc accagaaacg gtagcgagac gaaggtctgc gcaagcacga
     6661 cggcggttgt gctgaacgcg atgctgatcc cggcagcttc gaggtaccgg ccgatgagcc
     6721 ccagccggcc gaacgcgtag aggagggcaa tgccccccac caccggcggc agcaccagcg
     6781 ggagcaggat cagcgggcg
//
LOCUS       DASSGQ010000151         2894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_952823,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000151 DASSGQ010000000
VERSION     DASSGQ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952823"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /gene="pdhA"
                     /locus_tag="VFI55_06835"
     CDS             <1..1045
                     /gene="pdhA"
                     /locus_tag="VFI55_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HET7073781.1"
                     /translation="QLVAADGTPTAERRYSRDLPKETLCWLYEMMMVTRELDTEFVNL
                     QRQGELALFTPCRGQEAAQVGAAACLRKTDWLFPQYRELGAYLVRGIPPGHVGAAWRG
                     TWNGGLEFTKKCCAPIAVPVGTHTLHAVGAAMAAQRLGEDSVTVAFLGDGATSEGDVH
                     EALNFAGVFATPCVFYVQNNQWAISVPVRKQTAAPSIAHKAIGYGMPGVRVDGNDVLA
                     CYAVMAEAAARARAGDGPTLIEAVTYRLGPHTTSDDPTRYRTQEEVDHWLALDPIPRY
                     RKYLQGQGLWSERLEQRVAAHAARQRAELRDAVFGAPDFGIDDVFTTVYAEITPGLEA
                     QRQQLRAELARTE"
     gene            1056..2111
                     /gene="bkdB"
                     /locus_tag="VFI55_06840"
     CDS             1056..2111
                     /gene="bkdB"
                     /locus_tag="VFI55_06840"
                     /EC_number="1.2.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HET7073782.1"
                     /translation="MTQIADRPARPGETVTAAVENVALAEQPLTMVQALNRALHDAMA
                     ADDRVLVFGEDVSVEGGVFRVTEGLAETFGEDRCFDTPLAESAIIGIAVGLALRGFVP
                     VPEIQFDGFSYPAFDQVVSHLAKYRTRTRGEVNMPVTVRLPSFGGIGAAEHHSESTEA
                     YWAHTAGLKVVVPSTPADAYWLLRHAIDCPDPVMYLEPKRRYWGRGMVDTGQPEPPIG
                     RAMVRRPGTDVTVVTYGALVTTAVTTAEEAQRQRGWSLDVIDLRSLVPLDFDTIAASI
                     HRTGRCVVLHEGPRSLGYGAGLAARIQEELFYELEAPVLRACGFDTPYPPARLERLWL
                     PGPDRLLDCVERVLELP"
     gene            2108..>2894
                     /locus_tag="VFI55_06845"
     CDS             2108..>2894
                     /locus_tag="VFI55_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET7073783.1"
                     /translation="MSGPPDHQRVKSFRVPDLGEGLAEVTVTCWNVAAGDEVELNQTL
                     CSVETAKAEVEIPSPYAGRVVETHGAEGDVLQVGAVLVRIDTAPANPDVAASNGHTPA
                     PTLVGYGADAGIDASRRTGRPLAAPPVRKLAKELMVDLASLPGNAGDVITRADVLAAA
                     HGSGVAPDIRPVRGVQARMAEKMAVSHKEIPAAKVSVDVECAALLQLRDRFRVVHPGI
                     TPFVLTLRLLVIALTHNKVLNSTWVDSPEGPQVHLHRGVHLGFG"
BASE COUNT          463 a          943 c         1017 g          471 t
ORIGIN      
        1 gcaacttgtc gctgcggacg gcacgccgac ggccgaacgg cgctacagcc gtgaccttcc
       61 caaggagacc ctgtgctggc tctacgagat gatgatggtc acccgcgagc tggacaccga
      121 attcgtcaac ctgcagcgtc agggcgagct ggcgctgttc acgccctgcc gcgggcagga
      181 ggcggcgcag gtcggtgcgg cggcctgtct gcgcaagact gactggttgt tcccccaata
      241 ccgtgaactg ggcgcctatc tggtgcgcgg catcccgccc ggacacgtgg gggccgcgtg
      301 gcgcggaacc tggaacggcg gtttggaatt caccaagaag tgctgcgccc cgatagcggt
      361 tccggtcggc acccacaccc tgcatgccgt cggcgcggcg atggccgcgc aacgcctggg
      421 cgaggactcc gtaacggtgg ccttcctcgg agacggcgcc accagcgagg gcgacgtgca
      481 cgaggcgctg aacttcgccg gggtcttcgc caccccgtgc gtgttctacg tgcagaacaa
      541 ccagtgggcg atctcggtgc cggtgcgcaa gcagaccgcg gcaccttcga tcgcgcacaa
      601 ggcgatcggt tacgggatgc ccggcgtccg ggtggacggc aacgacgtgc tggcgtgcta
      661 cgcggtgatg gccgaagccg cagcccgagc ccgggccggc gacggcccga ccttgatcga
      721 ggcggtcacc taccggttgg gcccgcacac cacctccgat gatccgaccc ggtaccgaac
      781 tcaggaggaa gtcgaccact ggctcgcgct ggacccaatt ccgcgttacc gcaagtactt
      841 gcagggccag ggcctgtggt cggaacgcct ggagcaaagg gtcgccgcgc acgcggcgcg
      901 gcagcgggcc gaactgcgcg acgcggtgtt cggcgcgccc gatttcggca tcgatgatgt
      961 gttcaccacg gtgtacgccg aaatcacgcc cgggttggag gcgcagcgcc aacagctgcg
     1021 cgccgaactg gcccggaccg aatgaggagg cggacatgac ccagatcgct gatcgtccgg
     1081 cccgcccagg cgaaaccgtc acggcggcag ttgagaacgt cgcgctcgcc gagcaaccgt
     1141 tgaccatggt gcaggcgctc aaccgcgcgc tgcacgacgc gatggccgcc gacgaccggg
     1201 ttctggtgtt cggtgaggac gtctccgtcg agggtggggt gttccgggtc accgaaggac
     1261 tggccgaaac gttcggcgag gaccggtgct tcgacacgcc gctggcggag tcggccatca
     1321 tcgggatcgc ggtggggttg gcgctacgcg gttttgtgcc ggtgcccgag attcagttcg
     1381 acggcttttc ctacccggcg ttcgatcagg tcgtcagcca tctggcgaag tatcggaccc
     1441 gtacccgcgg tgaggtgaac atgcccgtca cggtccgact gccttcgttc ggtggaatcg
     1501 gtgcagccga acaccattcg gaatccaccg aagcttactg ggcacacacg gccggtctga
     1561 aggtggtggt cccgtcgaca cccgccgacg cctactggct gctccggcat gctatcgact
     1621 gtccggaccc ggtgatgtac ctggagccga aacggcgcta ctggggccgc ggaatggtcg
     1681 ataccggcca gccggaaccg ccgatcgggc gggcgatggt gcgccggccc ggcaccgatg
     1741 tgacggtggt gacctacggc gccctggtga cgaccgcggt gacgaccgcg gaagaagccc
     1801 agcgccaacg tggttggagt ctggatgtca tcgatctgcg gtcactggtg ccgttggatt
     1861 tcgacaccat cgccgcgtcg atccatcgga cggggcgctg tgtggtgctg cacgagggtc
     1921 cgcgcagcct gggctacggt gccggtcttg ccgcacgcat ccaggaagag ctgttctacg
     1981 agctggaggc accggtgctg cgcgcctgcg gttttgatac cccctatccg cctgcgcggt
     2041 tggagcgatt gtggttaccg ggccccgacc ggctgctgga ctgcgtcgaa cgcgtgctgg
     2101 agctgccgtg agcggcccac ccgatcacca gcgggtcaag tcgttccggg tccccgatct
     2161 cggcgaggga ctcgcggagg tgaccgtgac gtgctggaac gttgcggccg gtgacgaggt
     2221 cgagctgaat cagaccctgt gctcggtgga gacggccaaa gccgaggtgg aaatcccgag
     2281 cccatatgcg ggccgggtcg tcgaaaccca cggtgccgaa ggcgatgtgc tccaggtggg
     2341 ggcggtgctg gtccgcatcg acaccgcgcc ggcgaacccg gacgtggccg cgtcgaacgg
     2401 gcatactccg gcgcccacgt tggtcggtta cggcgcagac gcgggtatcg acgccagccg
     2461 ccgaaccggc cgtccgctcg ccgccccgcc agtccgcaag ctggccaagg agctgatggt
     2521 cgacctcgcc tcgttacccg gcaacgcggg tgacgtgatc acccgcgcag acgtgctcgc
     2581 cgccgcccac gggtccgggg ttgcgcccga catccggccc gtccggggtg tgcaggcgcg
     2641 gatggcggag aaaatggcgg tgtcccacaa ggaaatcccg gccgcgaagg tcagtgtcga
     2701 cgttgaatgc gccgcgttgc tgcaactccg cgaccgtttt cgcgttgtcc acccggggat
     2761 cacaccgttt gtgctgacac tgcggttgct ggttatcgcc ttgacgcaca acaaggttct
     2821 caattcgacc tgggttgact cgcccgaagg cccccaggtg cacctccacc gtggcgtgca
     2881 cctggggttc gggg
//
LOCUS       DASSGQ010000152        13090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_770375,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000152 DASSGQ010000000
VERSION     DASSGQ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13090
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770375"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VFI55_06850"
     CDS             complement(<1..571)
                     /locus_tag="VFI55_06850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7073784.1"
                     /translation="MARTDNDTWDLATSVGATATMVASGRARATRAALIDDRFAEPLV
                     RAVGVDFFTRWATGELDSTDVDVPGAPWGMQRMTDIQAARTRYIDAFFSEAAAAGIRQ
                     VVILAAGLDARGYRLPWPPGTKVFEIDQPQVIEFKTATIGALGAEPTADVRAVPIDLR
                     HDWPAALRQAGFDSSRPAAWAAEGLLGFLP"
     gene            732..1391
                     /locus_tag="VFI55_06855"
     CDS             732..1391
                     /locus_tag="VFI55_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET7073785.1"
                     /translation="MDITTTIAELPCPAPVVGDAEWRAGARWARQLAWISLVVVLIEG
                     AIGLWQGLAVGSVALTGWALGGGSEALASAMVVWRFSGARTLSQTAESRAQRGVAVSF
                     WLTAPYVAAESLRHLTGAHHVDTSVIGIGLTAVSLLLMPILGRANHNVGERLGSEATE
                     GAGMQNYLCAAQAAGVLAGLAVTASCPGGWWFDPAIGLGVAAVAVWQGIRSWRGHACG
                     C"
     gene            complement(1406..3280)
                     /locus_tag="VFI55_06860"
     CDS             complement(1406..3280)
                     /locus_tag="VFI55_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7073786.1"
                     /translation="MRAVTAPLQSHDFTGTAVRLLKRIAPQRRPVIAAIVLSIIATAI
                     SAVGPRVLGHATDLIFDGVIGRRLPAGITKEQAVAAARARGENTFADMLSGMNVAPGS
                     GVDFGAVGRTLLLALGIYLSAALLVWVQNRILNAIVQRTMRALRCDVEDKVHRLPLSY
                     FDGQQRGELLSRVTNDIDNIQASVSMTIGQLVSAMLTVVAVLAMMISISPLLAGIALA
                     AVPLSLVATQAIVRRSRRHFVAQWVNTGRLGAHIEETYSGFTVVKTFGHRAEALSRFR
                     ELNDELYQTSFGAQFFSGLISPATTFIGNLSYVAAAVVGGLQVTTGRMTLGSIQAFVQ
                     YVRQFNQPLAQVAGMYNTLQSGMASAERVFDFLDEPEESADSDLVLPALDGQGRRGRV
                     EFQRVGFAYRPGTPVINDLSVVAEPGSTVAIVGPTGAGKTTLVNLLMRFYEVDSGLIL
                     IDGVDIASVSRDSVRSRIGMVLQDPWLLDGTIAENICYGRPGARDDEMVAAARAVHLE
                     GFVGTLPEGYQTQVGCNGGNISAGQRQLITIARAFLARPQLLILDEATSSVDSGTELQ
                     IQRAMSALRANRTSFIVAHRLSTIRDADHILLVEAGKVVEQGTHAELLARRGAYYRMT
                     "
     gene            complement(3284..3988)
                     /locus_tag="VFI55_06865"
     CDS             complement(3284..3988)
                     /locus_tag="VFI55_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073787.1"
                     /translation="MFTLREHNVEDYATLRDMDTTDVAVLDEADRACLDELGEYLVSA
                     DASQRFAIWLLHKHFEPAAGEVFVESLSTAPRGTRTTLLERCSALDLHATSMRFDSDV
                     SSGVGVVGMEFADPADFGSTTSVSPDDEAILTGLAERLRARGKIERFGVRLIRDPLGL
                     SDDEVLVETCDIAHRTLHCSVAPRGAGTNSVETTWQWKPSLSKTGPTVHRYCMNQCTL
                     DSGNNHYISGHTNYPD"
     gene            complement(4345..6561)
                     /gene="ctpC"
                     /locus_tag="VFI55_06870"
     CDS             complement(4345..6561)
                     /gene="ctpC"
                     /locus_tag="VFI55_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009955372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-exporting P-type ATPase CtpC"
                     /protein_id="HET7073788.1"
                     /translation="MNLAVAHEAEAANDPELVVVSDAAGRMRVSVDWVRGNSRRAVAV
                     EEAVAKQNGVRAVHAYPRTGSVVVWYSPRRCERALVLAAISGAAHVAAELIPARAPHS
                     SEIRNTDVLRMVIGGLALTLLGVRRYVFVRPPLLGPSGRLVATGVTVFTGYPFLRGAL
                     RSLRSGKAGTDALVSAATVASLILRENVVALTVLWLLNIGEYLQDLTLRRTRRAISEL
                     LRGSQDTAWIRLTVGAQAGREMTGDPGAATEIQVPIDSVQIGDEVVVHDHVAIPVDGE
                     VVEGEAIVNQSAITGENLPVSVAVGAHVHAGSVVERGRLVVRADAVGSQTAIGRIIAR
                     VEEAQHDRAPIQTVGENFSRRFVPTSFIVSAITLAITGDVRRAMTMLLIACPCAVGLA
                     TPTAISAAIGNGARRGILIKGGSHLEQAGRVDAIVFDKTGTLTVGRPVVTNIVAMHKD
                     WQPEQVLAYAASSEIHSRHPLAEAVIRSTEERHISIPPHEECEVLVGLGMRTWADGRT
                     LLLGSPSLLRAEKVRVSRQAAAWVDKLRRKAETPLLLAVDGTLVGLISLRDEVRPEAA
                     EVLTKLRANGIRRIVMLTGDHPEIARVVAGELGIDEWRAEVMPEDKLEVVRELQNDGF
                     VVGMVGDGINDAPALAAADIGIAMGLAGTDVAVETADVALANDDLHRLLDVRDLGARA
                     VDVIRENYGMSIAVNAAGLIIGAGGALSPVLAAILHNASSVAVVANSSRLIRYRLD"
     gene            complement(6571..6852)
                     /locus_tag="VFI55_06875"
     CDS             complement(6571..6852)
                     /locus_tag="VFI55_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1490 family protein"
                     /protein_id="HET7073789.1"
                     /translation="MAVYGLFAKAASTVVTGLVGVTAYELLRKALATAPLHETAVSAA
                     ELGLRGTRRAEEAAESARLKLADVMAEARERIGEEAPTPSITDTHQHEH"
     gene            7110..8540
                     /locus_tag="VFI55_06880"
     CDS             7110..8540
                     /locus_tag="VFI55_06880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011595642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HET7073790.1"
                     /translation="MAFPMHPADAVFLVAETPNRPMHMGALALLSPPDGAGRTFVRDT
                     FAAALDRDRVAPLWRRRPRRSLSSLGQWYWHTDAEVDLDYHVQVSALPQRAGANMLWD
                     MVSDLHAEVLDLSRPLWQMHLIEGLPDGQYALYIKTHHALADGVSTMRLLRRTVSWDP
                     DRRGMPALWEASPHPQGDDTIAAPRVSPLAAARWVLGAAGDIAGVPPALADTAWRALR
                     RRGGPLTLTAPDTPFSAAIDGTRSFAGCAFPIDRLRLVAKHTDATLNDVVLAMCGGAL
                     RRYLLARHALPAAPLMASVPVSLRGDEPVGAPDPMPGNKIGALACSLGTHLADPAERL
                     AVVQASMRQGKAALAGRSRLQVLAMSAVGATPLALTMTLGRTPGPLRPPNVMISNVPG
                     PKDPLYWNGARFDALYPLSVIADGQALNITCTSFNDQISFGLTGCRRVVPRIDTIADA
                     LGRELDLLDAFHRIPQTGRGRRRNPR"
     gene            complement(8565..9308)
                     /locus_tag="VFI55_06885"
     CDS             complement(8565..9308)
                     /locus_tag="VFI55_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003899994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03089 family protein"
                     /protein_id="HET7073791.1"
                     /translation="MHQLSTLTTLSGAILDPILRADPVGPRITYYDDATGERIELSAV
                     TLANWAAKTANLLRDELGAGPASRIAILLPAHWQTAAVLFGVWWIGAEAVLGAGEPAD
                     LALCTAERLDEADSALTDTAPGGEVAVLSLDAFGRPAPDLPIGVTDYATAVRVHGDQI
                     VPERHPGPALAGRSVDEILADCANSAAARELTAKDRVLSSASWTRPGELVDGLLSILA
                     VGASLVQVANPDPEALQRRIETEKVTRVL"
     gene            complement(9296..10777)
                     /locus_tag="VFI55_06890"
     CDS             complement(9296..10777)
                     /locus_tag="VFI55_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET7073792.1"
                     /translation="MPVQRVVRVVVTVLTIAVVLGTGFAWSNVRSFEDGIFHMSAPSL
                     GKGGDDGAIDILLVGLDSRTDAHGNPLTAEELETLRAGDDESTNTDTIILVRIPNNGN
                     SATAISIPRDSYVQAPGLGKTKINGVYGQTREAKRASLVKAGDSAADAAAQGTQAGRD
                     ALIKTVADLTGVTVDHYAEIGLLGFALITDALGGVDVCLKEPVFEPLSGADFPAGQQK
                     LNGAEALSFVRQRHDLPRGDLDRVVRQQVVMASLAHRIISGKTLSSPATVKRLESAVQ
                     RSVVISSGWNVMDFVQQLSKLAGGKVAFATIPVLDGAGWSDDGMQSVVRVDPHQVADW
                     VGGLLNDQAEGKTEQLAYTPAKTTASVVNDTDINGLAAAVSDVLSSKGFSTGTVGNND
                     GGHVKASQVRAAKSDDVGAQEVAKELGGLPVVADTSLAVGSVRVLLANDYTGPGSGLG
                     GGIPAVPARASNAGSSAGSGADANVPPPSPILTAGSDKPECIN"
     gene            10855..11772
                     /gene="rfbD"
                     /locus_tag="VFI55_06895"
     CDS             10855..11772
                     /gene="rfbD"
                     /locus_tag="VFI55_06895"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HET7073793.1"
                     /translation="MSGRIVIAGAGGQLGTSLRALTTGQGRDVLALTSSQWDITDPAA
                     AERIVQNGDVVVNCAAYTDVDGAESDEAGAYAVNAAGPEHIARVCARVGARLIHVSTD
                     YVFSGEPFTGDSGVRNPRPYEPSDDTDARGVYARSKLAGERAVLAALPEAVIVRTAWL
                     YTGGTGKDFVAIMRRLAAGDGPVNVVDDQTGSPSYVADVAAALLQVADENVPGPILHA
                     ANEGVVSRYEQARAVFEECGADPARVRPVSTDQFPRPAPRPTYSALSGRESAAAGLTP
                     LRPWRPALVAALAASPANATDRPIPSTRD"
     gene            11735..12664
                     /locus_tag="VFI55_06900"
     CDS             11735..12664
                     /locus_tag="VFI55_06900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003899992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7073794.1"
                     /translation="MPPTDRYPRRVTDVLPVVTVTYSPGPHLERFLASLSLATERPVS
                     VLLADNGSVDGTPEAAVERYPNVRLFDTGANLGYGTAVNRAVAQLDEDLDVNGDWVIV
                     ANPDVQWGPGSIDAMLDAATRWPGAGALGPLIRDPDGSVYPSARHLPSLLRGGMHAVV
                     GPFWKRNPWTKAYRQEHLEPSERPVGWLSGSCLLVRRSAFGQVGGFDERYFMYMEDVD
                     LGDRLGKAGWLSVYVPSAEVLHHKGHSTGHDPASHLAAHHTSTYIFLADRNSGRWRAP
                     LRWTLRASLAVRSRLMVRSSLRSRRRDLADGRH"
     gene            12666..>13090
                     /locus_tag="VFI55_06905"
     CDS             12666..>13090
                     /locus_tag="VFI55_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET7073795.1"
                     /translation="MAIPQVDAVVLVGGKGTRLRPLTLSAPKPMLPTAGLPFLTHLLS
                     RIASAGIEHVILSTSYQAAVFEAEFGDGYKLGLEIEYVTEERPLGTGGGIANVADYLR
                     YDSAMVFNGDVLSGAELCQVLQFHQTNEADVTLHLVRVG"
BASE COUNT         2022 a         4467 c         4445 g         2156 t
ORIGIN      
        1 acggcagaaa gccgagcagg ccctcggcgg cccaggcggc gggtcggctg ctgtcgaacc
       61 cggcctgtcg cagcgccgcc ggccagtcgt gacgcaggtc gatcgggacg gcccggacgt
      121 ccgccgtggg ttcggcgccc agcgcgccga tcgtggcggt cttgaattcg atgacttgcg
      181 gctggtcgat ttcgaagacc ttggttcccg gcggccaggg caggcggtat ccgcgtgcgt
      241 cgaggccggc agccaaaatg accacctgac ggatgcccgc ggcggccgcc tcggagaaga
      301 acgcgtcgat gtagcgggtg cgcgcggcct gaatgtcggt catgcgttgc atgccccacg
      361 gcgcaccggg gacgtcgaca tcggtggagt cgagttcgcc cgtggcccac cgggtgaaga
      421 agtcgacgcc cacggcgcgc accagtggct cggcgaaccg atcgtcgatc agcgccgccc
      481 gggtggcccg ggctctgccc gacgccacca tggtggcggt ggcccccacg ctggtcgcca
      541 ggtcccaggt gtcgttgtcg gtacgcgcca cgatgtcccc tctccgatgc actccttagc
      601 aagaataact acaggcgcgt ggccgggatc ccaccgcggt gccgaagcgg tagcacaaca
      661 acctggtaac gaccgctggg ccgccacgcg gggctccggc cgacgggtcg tttcgcttct
      721 accatcgccg gatggacatc acgaccacga tcgccgagct gccgtgtccc gcgccggtcg
      781 tgggggatgc cgagtggcgg gccggcgccc gatgggcgcg gcagttggcc tggatcagcc
      841 tggtcgtggt gctcatcgag ggcgcgattg gactctggca gggcctggcg gtcgggtccg
      901 tcgcattgac tgggtgggca ctcggcggcg gttctgaggc gctggccagc gcgatggtgg
      961 tatggcgctt cagcggcgct cgcacgctgt cgcagaccgc cgagtcgcgc gcacagcgcg
     1021 gcgtcgcggt gtcgttttgg ctgaccgccc cgtacgtcgc ggccgaatcc ctccgccacc
     1081 tgaccggcgc gcaccatgtc gacacctccg tgatcggcat cgggttgacc gcggtctccc
     1141 tgttgctgat gccgattctc ggccgggcca accacaacgt gggcgagcgg ctgggctcgg
     1201 aggccaccga gggtgcaggc atgcagaact acctctgcgc ggcccaggcc gccggggtgc
     1261 tggccggcct cgccgtcacg gccagctgtc cgggaggttg gtggtttgac cccgcgatcg
     1321 gccttggtgt ggccgccgtc gcggtgtggc aaggaatccg ctcctggcgt ggtcacgcct
     1381 gcggctgctg atcgttgacc gcggttcacg tcatgcggta gtaggcaccc cgccgggcca
     1441 gcagttcggc gtgggtgccc tgctcgacga ccttgccggc ctccaccagc aggatgtggt
     1501 cggcatcgcg gatcgtcgaa aggcggtgcg cgacgataaa actcgttcgg ttggcgcgca
     1561 gtgcggacat cgcacgctga atctgcagct cggtgccgct gtccaccgaa ctggtcgcct
     1621 cgtcgaggat caacagctgt gggcgggcga gaaacgctcg cgcaatggtg atcaactgcc
     1681 gctggccggc gctgatgttg cctccgttgc agccgacctg cgtttggtag ccctctggga
     1741 gggtgcccac aaaaccctcc aggtggaccg ctcttgccgc cgcgaccatc tcgtcgtcgc
     1801 gcgcgccggg ccgcccgtag cagatgttct ccgcgatcgt cccgtcgagc agccagggat
     1861 cttgcagcac catgccgatc cgcgaccgca ccgagtcccg gctcaccgat gcgatgtcga
     1921 cgccgtcgat caggatgagg ccggagtcaa cctcgtagaa ccgcatcagc aggttcacca
     1981 gtgtggtttt gcccgcgccc gtcggcccga cgatcgccac tgtgctgccg ggctcggcca
     2041 ccaccgacag gtcgttgatc acgggggtgc cggggcggta ggcgaaaccg acgcgctgga
     2101 attcgacccg cccccgccga ccctgaccgt cgagcgccgg cagcaccagg tcgctatccg
     2161 ctgattcctc gggctcgtcg aggaagtcga aaacccgctc ggcgctggcc atcccggact
     2221 gcaatgtgtt gtacatgccg gcgacctggg ccagcggctg gttgaactgg cgcacgtact
     2281 gcacgaacgc ctggatgctg cccagcgtca tccggccggt tgtcacttgc agaccaccga
     2341 ccaccgccgc tgcgacgtag ctgaggttgc caatgaaggt ggtggccggc gatatcaggc
     2401 cggagaagaa ctgggcgccg aaactggtct gatacagctc gtcgttgagc tcgcggaacc
     2461 gcgatagcgc ctcggcccgg tggccgaacg tcttcaccac ggtgaaaccg ctgtaggtct
     2521 cctcgatgtg ggcgccgagc cgtccggtgt tgacccactg ggcgacgaag tgccgccgcg
     2581 accgtcgcac gatcgcctgg gtcgccacca gcgaaagtgg cacggccgcc agcgcgatgc
     2641 ccgccagcag cggcgagatg gagatcatca tcgcgagcac ggccaccacc gtcagcatcg
     2701 cggacaccaa ctggccgatg gtcatcgaga cggacgcctg gatgttgtcg atgtcgttgg
     2761 tgacccggct caacagttcg ccacgctgct ggccgtcgaa gtaggacagc ggcagccggt
     2821 gcactttgtc ctcgacgtcg cagcgcagcg cccgcatggt tcgctgcacg atcgcgttca
     2881 ggatccggtt ttgcacccaa accagcagtg cggcagaaag atatatgccc aatgcgagca
     2941 acagcgtcct gccgaccgcg ccgaagtcca cgccgctccc gggcgccacg ttcatcccgg
     3001 acagcatgtc ggcgaaggta ttctcgccgc gggcgcgggc ggccgcgacg gcctgttcct
     3061 tggtgattcc ggccggcagt cggcggccga tgacgccgtc gaagatcagg tcggtggcgt
     3121 ggcccaggac ccgggggcca accgccgaga tcgccgttgc gatgatggac agcacgatcg
     3181 cggcgatgac cggcctgcgt tgcggcgcaa tgcgtttcag tagacgaacc gcggttccgg
     3241 tgaaatcgtg agactgcagg ggggccgtca cggcgcgaat cggtcagtcc gggtagttgg
     3301 tgtggcccga gatgtaatgg ttgttgcccg agtccagtgt gcactgattc atgcagtacc
     3361 gatgcaccgt gggcccggtc ttgctcaggc ttggcttcca ttgccaggtc gtctccacgg
     3421 aattggtgcc ggcgccacga ggggcgacgc tgcagtgcag ggtccggtgg gcgatgtcgc
     3481 aggtttcaac gaggacctca tcgtccgaca ggccaagcgg atcgcggatc aaccgcacgc
     3541 cgaatcgctc gatcttgccg cgggcgcgca gccgttcggc gaggccggtc aatatggcct
     3601 cgtcgtcggg gctcaccgac gtggtggagc cgaagtcggc cgggtcggcg aactccatac
     3661 cgacgacgcc gacaccggaa ctgacgtcgg agtcgaaacg catcgatgtc gcgtgcaaat
     3721 ccagcgcgga gcaacgctcg agcagcgtgg ttcgggttcc gcgcggcgcc gtgctgaggc
     3781 tctccacaaa gacctcgccg gcggccggct caaagtgctt gtgcagcagc cagatcgcga
     3841 atcgctgcga tgcgtcggcc gagacgagat attcgcccaa ttcgtcgaga caggcgcgat
     3901 cggcctcgtc cagcacagcg acgtcggtgg tgtccatgtc gcgtaacgtc gcatagtcct
     3961 caacgttgtg ctcccgcaag gtgaacatcg ctttcctttc tacgtccggt cgaaggccag
     4021 aattgggcga aacggctgca actactacga aatgcacacg gcaacaaaat tcttcggcgc
     4081 tcaggcatct cgccggtggg ccgcaacaac gaacgtgtcg ccgccttcgg caagatgacc
     4141 tgccaccgga ctctaccgag cgatctggga cctaacaatt atgcggcgct gtgagaacct
     4201 gtggattggt ttttggccga gtggctaagg gcgccacact ttctcggtgg tctcgattca
     4261 atccgcgacg aaaatgtggc cgcgattcgg gcgctcgggc atgccgatga cgcccgttgc
     4321 cgattcgcga catcgggtcg cggatcagtc cagccggtac cggatgagcc gggagctgtt
     4381 ggccaccacc gccaccgacg acgcgttgtg caggatcgcg gccagcaccg gggacagtgc
     4441 gccgcccgcg ccgatgatca gcccggccgc gttgacggcg atggacatgc cgtagttctc
     4501 gcggatcacg tcgaccgccc gcgcgcccag gtcgcgcacg tcgagcaggc gatgcagatc
     4561 gtcattggcc aacgccacgt cggcggtctc gacggcgacg tcggttccgg ccagccccat
     4621 ggcgatcccg atatcggcgg ccgccagcgc cggcgcgtcg ttgatgccgt cgccgaccat
     4681 gccgacgacg aacccatcgt tttgcagctc gcgcaccacc tcgagcttgt cctccggcat
     4741 cacctcggcg cgccactcgt cgatcccgag ctctccggcg acgacccgcg cgatctccgg
     4801 gtgatctccg gtgagcatga cgattcggcg aatcccgttg gcccgcaact tcgtcagcac
     4861 ctcggcggct tccggccgca cctcgtcccg caggctgatc agccccacca gcgtgccgtc
     4921 caccgccaac agcaacgggg tctcggcctt gcgtcgcagc ttgtccaccc acgccgcggc
     4981 ctgcctcgac accctcacct tctcggcgcg cagcagcgac gggctgccca gcagcagggt
     5041 gcggccgtcg gcccaggtcc gcatgcccag gcccaccagc acctcgcact cctcgtgcgg
     5101 cgggatgctg atgtggcgtt cctcggtgga acgaatgacg gcttcggcca gcgggtgacg
     5161 ggagtggatc tccgagctgg ccgcatacgc gagcacctgc tcgggctgcc aatccttatg
     5221 catggcaacg atattggtga ccaccggacg gccgacggtc agtgtcccgg tcttgtcgaa
     5281 gacgatcgcg tcgacacggc ccgcctgctc gaggtgcgat ccgcccttga tcaggatgcc
     5341 gcggcgcgcg ccgttgccga tcgccgcact gatcgccgtc ggggtggcca atccgaccgc
     5401 gcacgggcag gcgatcaaca gcatggtcat cgcgcgccgc acgtctccgg tgatcgccag
     5461 ggtgatcgcc gaaacgatga acgacgtggg aacgaaacgg cgagagaagt tttcgccgac
     5521 cgtctggatg ggcgcgcggt cgtgctgtgc ctcctcgacc cgggcgatga tgcggccgat
     5581 cgcggtttgg ctgccgacgg cgtcggcgcg caccaccaga cgtccgcgct ccacgaccga
     5641 accggcgtgc acgtgggcgc cgaccgcgac actgacgggt aagttctctc cggtgatcgc
     5701 ggattggttg acgatcgcct cgccttcgac cacctcgccg tccaccggta tcgcgacgtg
     5761 gtcgtggacg accacctcat cgccgatctg cacgctgtcg ataggcacct gtatctcggt
     5821 ggcggccccc gggtcgccgg tcatctcgcg gcccgcctgc gcgcccacgg tcagccggat
     5881 ccacgccgtg tcctggctgc cgcgcagcag ttccgagatg gcccgccggg tccggcgcag
     5941 ggtcaggtcc tgcaggtact cgccgatatt gagcagccac agcacggtga gcgcgaccac
     6001 gttctcgcgc aggatcaggc tcgccacggt cgccgccgag accagtgcgt cggtgccggc
     6061 cttaccggag cgcagcgagc gtagcgcgcc gcgcaggaag gggtagccgg tgaagaccgt
     6121 cacgccggtg gcgaccaggc ggccactcgg gccgagcagc ggcggtcgca cgaacacgta
     6181 ccggcgcacg ccgagcaggg tcagcgccag gccgccgatg accatgcgaa gcacatcggt
     6241 attgcggatc tccgacgaat gcggtgcacg ggccgggatc agctcggcgg cgacgtgcgc
     6301 ggcgccgctg atggctgcca gcaccagggc gcgttcgcag cgccgtggcg aataccagac
     6361 gacgacggat ccggtgcgcg ggtaggcgtg cacggcgcgc acgccgttct gcttggcaac
     6421 ggcctcttcg accgcgacgg cgcgccggga gttgccgcga acccagtcga cggaaacccg
     6481 catgcgtcca gcggcatccg aaaccaccac cagttcagga tcgttggcgg cctcggcctc
     6541 gtgagcgacc gcgaggttca tctcgcgtga tcagtgctcg tgctgatggg tgtcggtgat
     6601 ggacggggtg ggcgcttctt cgccgatgcg ttcgcgggcc tcggccatga cgtcggcgag
     6661 cttcagccgc gccgactcgg cggcctcctc ggccctgcgg gtaccgcgca atcccagctc
     6721 agccgccgac accgcggtct cgtgcagcgg cgccgtggcc aaggccttgc gcaaaagctc
     6781 gtaggccgtc acgccgacca ggccggtgac taccgtcgat gccgctttag cgaagagtcc
     6841 gtacaccgcc attgcctgaa acctcctcat tacgccggca tcggccgcct cgcgtcgaca
     6901 ttaacataca tatattgcga taccaatatg cattcaggcc gtcctgcggt tgccgcatcg
     6961 ggcaatatga gcatattgtt attgcacgtc cgccagttct cggaggcttc ggccgtccgg
     7021 aagggggcgg caatcgaaat ccgcgtgttc cgtggagggg aacagccgca cggcgcgaca
     7081 tttcggttta ccgagaggtg ctgccgcaca tggcttttcc tatgcatcca gccgacgcag
     7141 tgttcctggt cgctgagacg cccaaccgtc cgatgcacat gggtgcgctg gcgctgctga
     7201 gcccgcccga cggcgccggc cggactttcg tgcgagacac gttcgcggcc gcgctggatc
     7261 gcgaccgggt ggcgcccttg tggcggcgcc ggccccgccg ctcactgagt tcccttggtc
     7321 agtggtattg gcacaccgac gccgaggtcg acctggatta ccatgtgcag gtcagcgctc
     7381 tgccgcagcg agccggcgcc aacatgctgt gggacatggt ttccgatctt catgccgaag
     7441 tgctggacct gtcccgtccg ctgtggcaga tgcatttgat cgaaggcctc cccgacggtc
     7501 agtacgcgct ttacatcaag acccaccatg cgctggccga cggcgtctcg acgatgcggc
     7561 tgctgcggcg caccgtcagc tgggaccccg atcggcgcgg gatgcccgcc ctttgggagg
     7621 cgtccccgca cccccagggc gatgacacga tcgcagcgcc acgtgtcagc ccgctggccg
     7681 cggcccggtg ggtgttgggc gcggcgggcg acatcgcggg cgtgccgccg gcactggccg
     7741 acaccgcgtg gcgggccttg cgccggcgcg gcgggccgtt gacccttacc gcgccggaca
     7801 ctccattcag tgctgcgatc gacgggaccc gttccttcgc gggctgcgcc ttcccgatcg
     7861 atcggttgcg gctggtcgcc aaacacactg acgccaccct caacgatgtg gtcctcgcca
     7921 tgtgcggagg tgcgctgcgc cggtatctcc tcgcgcgtca cgcgctgccc gccgcgccgc
     7981 tgatggcgag cgtgccggta tcgctgcgtg gtgacgaacc cgtgggcgcg ccagacccga
     8041 tgccgggcaa caagatcggt gcgttggcct gcagcctggg cacgcatctg gccgatcccg
     8101 ccgagcgtct tgcggtggtg caggccagca tgcgccaggg gaaggccgca ctggccggcc
     8161 gaagccggct gcaggtgctg gccatgagtg cggtcggcgc cacgccgctc gcactgacca
     8221 tgacgctggg ccgcacaccc ggaccgctac gcccgccgaa cgtgatgatt tccaacgttc
     8281 cgggacccaa ggacccgctt tactggaacg gcgcgcgctt cgatgcgctc taccctctgt
     8341 cagtaatcgc tgacggtcag gcgctcaaca tcacttgcac gagcttcaac gaccagatct
     8401 ccttcggcct gaccggctgc cgtcgcgtcg ttccgcggat cgacacgatc gccgatgccc
     8461 tgggccgcga actagatctg ctcgacgctt tccaccgaat accgcagacg ggtcggggtc
     8521 gccggcgcaa tccgcgataa cgtcaggtgt acgaacgcca ggactcacag aacccgggtg
     8581 accttttcgg tctcgattct gcgctgcagc gcctcgggat cgggattggc cacctggacc
     8641 aacgacgcgc cgacggccag gatcgacagc agaccgtcca ccagctcgcc gggccgcgtc
     8701 catgacgcgc tggaaagcac tcggtccttc gccgtcaatt cccttgccgc ggcggaattc
     8761 gcgcagtcgg cgaggatctc gtcgaccgat cggcccgcca gcgccggacc cggatggcgt
     8821 tcgggaacga tctgatcgcc gtgtacccgt accgcggtgg cgtagtcggt gacaccgatc
     8881 ggcaggtcgg gggccgggcg gccgaacgca tccagcgaca gcaccgccac ctcgccgccg
     8941 ggagccgtgt cggttagggc cgaatcggcc tcgtccaggc gttcggcggt gcacagcgcc
     9001 aagtcggcgg gttcgccggc cccgagcact gcctcggcgc caatccacca cactccgaac
     9061 agcaccgccg ccgtctgcca gtgtgccggc agcaagatcg cgatgcggct ggccggtccg
     9121 gcgcccagtt cgtcacgcaa caggttggcg gtcttggccg cccagttggc cagcgtcacg
     9181 gccgacaatt cgatgcgttc gccggttgcg tcgtcgtagt aggtgatgcg cggaccgacc
     9241 gggtcggctc gcagtatcgg atccaggatg gccccgctga gtgtggtcaa cgtgctcagt
     9301 tgatgcattc cggcttgtcg gaacccgcgg tcaggatcgg cgacggcggc ggaacgttcg
     9361 catcggcacc cgagccggca ctcgagccgg cgttcgaggc ccgcgcgggg acggcgggga
     9421 tgccgcctcc aagccctgag cccggaccgg tgtagtcgtt ggcaagcagc acccggaccg
     9481 atcccacggc caacgaggtg tcggcgacga ccggcaaccc acccaattcc ttggcgacct
     9541 cctgagcgcc gacgtcgtcg gacttggcgg cgcgcacctg gctggccttc acgtggccgc
     9601 cgtcgttgtt gccgacggtc ccggtggaaa atcctttgga gctcagcaca tctgagacgg
     9661 ctgccgccag cccgttgatg tcggtgtcgt tgaccacgct ggcggtggtc ttggccggcg
     9721 tgtaggccaa ctgctcggtc ttgccctcgg cctgatcgtt cagcaggccg ccgacccagt
     9781 cggcaacctg atgcgggtcc acccgcacca cgctttgcat accgtcgtcg ctccagccgg
     9841 ccccatcgag caccggaatg gtggcgaagg cgaccttgcc gccggcgagc ttggacagct
     9901 gctgcacgaa atccatgacg ttccagcccg aggagatcac caccgaccgc tggactgcgc
     9961 tctccagccg cttcacggtg gccgggctgg acagcgtctt gccggagatg atccggtgcg
    10021 ccagcgaagc catcaccacc tgctggcgga ccacccggtc caggtcgccg cgcggtaggt
    10081 catggcgttg gcggacgaag ctgagcgctt ccgcgccgtt cagtttttgc tggccggccg
    10141 ggaagtcggc acccgagagc ggctcgaaca cgggttcctt gaggcagacg tcaaccccgc
    10201 cgagcgcgtc ggtgatcaac gcgaagccga gcagcccgat ttcggcgtag tgatcgacgg
    10261 tgactccggt gaggtcggcg accgtcttga tcagggcgtc gcgccccgcc tgggtgccct
    10321 gggcggcggc gtcggcggcg gaatcgccgg ctttcaccag gctggcgcgc ttcgcctccc
    10381 tggtttggcc gtagacgccg ttgatcttcg tcttgcccag cccgggggcc tgcacatagg
    10441 agtcccgggg aatggagatc gcggtggccg aattcccgtt gttgggtatc cggaccagga
    10501 tgatcgtgtc ggtgttggtc gattcgtcgt cgccggcccg cagcgtctcc agttcctcgg
    10561 ccgtcagggg gttgccgtgc gcatcggtgc ggctgtccag gccgaccagc agaatgtcga
    10621 tcgcgccgtc gtcgccgccc ttacccagcg aaggtgcgga catgtggaag atgccgtctt
    10681 cgaaggaccg gacgttgctc caggcgaacc cggtgccgag aacgacggca attgtcagca
    10741 cagtgacaac cacacgaacc acacgttgca caggcatctc accagactac ttgcgacgca
    10801 gttccttcgc gggtagccac cggcggcgtg cccgggccgt gccagactcg aaccatgtcg
    10861 ggcaggatcg tgatcgcagg tgccggtggc caactaggca ccagtctgag ggcgctgaca
    10921 actggtcagg ggcgcgacgt gctggcattg acgtcgtcgc aatgggacat caccgacccc
    10981 gccgcggccg aacggatcgt ccagaacggc gacgtggtgg tcaattgcgc ggcctacacc
    11041 gatgtcgacg gcgccgagag cgacgaggca ggcgcgtacg cggtcaacgc cgccggcccc
    11101 gaacacatcg cgcgagtgtg cgcgcgcgtc ggtgcccggc tgattcacgt ctcgaccgac
    11161 tacgtctttt cgggcgagcc ctttaccggc gattcgggtg tccgcaatcc gcgtccctac
    11221 gagcccagcg acgacaccga tgcgcggggc gtgtacgccc gcagcaagct cgccggcgag
    11281 cgggccgtgc tggcggcgct gccggaggcc gtgatcgtcc ggaccgcctg gctctacacc
    11341 ggcggaaccg gcaaggactt cgttgcgatc atgcgtcggc tggccgccgg ggacggcccg
    11401 gtgaacgtgg tcgacgacca gaccggatcg ccgagctacg tcgccgacgt ggccgccgcg
    11461 ctgctacagg tcgccgacga aaacgtgccc ggccccatcc tgcacgccgc caacgagggc
    11521 gtggtctccc gctacgagca ggcccgcgcg gtgttcgagg aatgcggtgc cgacccggcg
    11581 cgggtgcggc cggtcagcac cgaccaattt ccgcgaccgg cgccgcggcc gacctactcc
    11641 gcgctgtccg gccgggagtc cgccgcggcg ggcctcaccc ccctgaggcc ctggcgaccc
    11701 gcgcttgtcg cggcgctggc ggcgtctccc gccaatgcca ccgaccgacc gataccctcg
    11761 acgcgtgact gacgtcctgc cggtcgtgac ggtgacctat tcgccgggtc cgcacttgga
    11821 gcggttcctg gcctcgttat cgctggccac cgagcgcccg gtgagcgtgc tgctggcgga
    11881 caacggctcc gtcgatggaa cgcccgaggc ggcggtcgag cgctacccga atgtccggct
    11941 gttcgacacc ggcgccaacc tgggatacgg aaccgcggtg aatcgcgcgg tcgcgcagct
    12001 ggacgaggac ttggacgtga acggggactg ggtgatcgtc gccaaccccg atgtgcagtg
    12061 gggcccgggc agcatcgacg ccatgctgga cgccgcgacc cgctggcccg gcgccggcgc
    12121 cctgggcccg ctcatccggg atcccgacgg gtcggtgtat ccgtcggcgc gccacctgcc
    12181 cagcctgctc cgcggcggca tgcatgcggt cgtagggccg ttttggaagc gcaatccctg
    12241 gacgaaggcg taccgccagg agcacctcga gcccagcgaa cggccggtgg gctggctgtc
    12301 gggatcgtgt ctgctggtgc gccggtcggc ctttggccag gtcggcggat tcgacgagcg
    12361 ctacttcatg tacatggagg acgtcgacct cggtgaccgg ctcggcaaag ccggctggct
    12421 gagcgtctac gtgccatcgg ccgaggtcct gcaccacaag gggcactcca ccgggcacga
    12481 cccggcaagc catctggccg cccaccacac gagcacctat attttcctcg ccgaccggaa
    12541 ttccggtcgg tggcgggccc cgctgcgctg gacactgcgg gcctcgctgg cggtgcgctc
    12601 ccgcctgatg gtgcgtagct ctttgcgcag tcgcaggcgg gacttggcag acggaaggca
    12661 ttgagttggc gatcccccag gtcgatgcgg tggtcctggt cggtggcaag ggcactcgcc
    12721 tgcggccgtt gacgctctcg gcgcccaagc ccatgctgcc caccgccggg ctgcctttcc
    12781 tcacgcattt gctgtcgcgg atcgcctcgg ccggcatcga gcatgtgatc ctgagcacgt
    12841 cgtatcaggc cgcggtgttc gaggcggagt tcggcgacgg gtacaagctg ggactggaaa
    12901 tcgaatacgt gaccgaggag cggccgttgg gaaccggcgg cggcatcgcg aacgtcgccg
    12961 actacctgcg ctatgacagc gcgatggtgt tcaacggcga cgtgctgtcc ggcgccgagc
    13021 tgtgccaggt gctccaattc caccaaacca acgaggccga cgtcacgttg cacctggtcc
    13081 gggtgggcga
//
LOCUS       DASSGQ010000153         3696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_740255,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000153 DASSGQ010000000
VERSION     DASSGQ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740255"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFI55_06910"
     CDS             <1..318
                     /locus_tag="VFI55_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073796.1"
                     /translation="FAAKLDAAGKARGQRLAWSPAEQHQLEVLADAVDRRTMLSVAFD
                     GLPVDNVKDRVRLSGEIRQLDRLATNLSRQIDAGLNPKTPDSRRTVKAREAANVRWGK
                     TGA"
     gene            311..637
                     /locus_tag="VFI55_06915"
     CDS             311..637
                     /locus_tag="VFI55_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073797.1"
                     /translation="MPDVAVDGPAGGFHLAGSYAHALEKAAVRAAGLDPREHLTMSGE
                     AWRRECSAAMRRVERFMDDHDGEDVVVEAWRLRGPQFRSAAVPEWLASATSVRVRCDD
                     TIELAS"
     gene            1258..2313
                     /locus_tag="VFI55_06920"
     CDS             1258..2313
                     /locus_tag="VFI55_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073798.1"
                     /translation="MTALWPRMPGEPPADRYVVRAGVLPDGWEGCPVFARPADVAEAL
                     RCRDATSAARMAGCPEDEALFVAEDPNNRHDSMRFTDPGRLVLRRVISRDWALRAAAR
                     FRPVVGAAFDYLALRGEVDACVGDDLLPRLIRWVIFAPGVDSWADVAAQVPVRRAEGA
                     QGDDLVSRVIRFAPQLSDQEVFVLSASSFGVAGGAVGFIGTLFTSTLAFLGADTDVQR
                     RLRGNPSDIPVFVDEMARLKTTTPLVSRHVTADTQIGGVDLPKGSAFIVATGEASVGG
                     ENGERVNLSPRRHWGFSAGPYRCTGIHLVHATIQVAVEEFLARIPEFRLCDERVVATR
                     HPYGHTRFNSAPLAWDG"
     gene            2392..2811
                     /locus_tag="VFI55_06925"
     CDS             2392..2811
                     /locus_tag="VFI55_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073799.1"
                     /translation="MTEDALSWLAALPRAVQTALLANPSGDVPPDIITRMPSLVVKTY
                     WTSNPDAGRWELHRQYATAATDGRARLDRWWKSLPLATRDALVEHRDGLVPNEHRAYV
                     ADLGALGVAVYVGASSAGEFSLHPLVSAYLDLHAESA"
     gene            complement(2852..2977)
                     /locus_tag="VFI55_06930"
     CDS             complement(2852..2977)
                     /locus_tag="VFI55_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073800.1"
                     /translation="MNAAVKNAIALWQANHHDFGWVFALMSGEDRRAALQALGLK"
BASE COUNT          530 a         1101 c         1343 g          722 t
ORIGIN      
        1 ttcgccgcga agctcgacgc ggcggggaag gcccgcgggc agcggctcgc ctggtcaccc
       61 gccgagcagc accagctcga ggttctcgcc gacgccgtcg accgtcgcac gatgttgtcg
      121 gtcgcgttcg acggcctgcc ggtcgacaac gtgaaggacc gcgtccgcct gtccggcgag
      181 atccgccagc tggaccgctt ggcaacgaat ttgtcccgcc agatcgatgc tggcctgaac
      241 ccgaaaacgc cggattcacg gagaacggtc aaggcgcgtg aagcggcgaa cgtgcgttgg
      301 gggaagaccg gtgcctgacg ttgctgtgga tggcccggct ggcggttttc atctggccgg
      361 cagttacgcg cacgcgttgg agaaggccgc tgtccgtgcg gccgggctcg atccgcgtga
      421 gcatctcacc atgtctggcg aggcctggcg gcgtgaatgc tcggcggcga tgaggcgtgt
      481 ggagcggttc atggacgacc acgacggtga ggatgtcgtg gttgaggcgt ggcgcctgcg
      541 tggacctcag ttccgttcgg ccgcggtgcc tgaatggttg gcgtcggcga cgtcggtgcg
      601 ggtgcgttgt gacgacacga tcgagctggc gtcgtgaccc ggcggtggcg ttgtcgccgt
      661 tggtcggtcc tagaggctcg gattgtcaag ttggagcggc ggatcgcgtt gctggaggct
      721 gggccgcgtc ctaggccgcc gttgctggtg ttcgggccgc cggtgtcgtg ttgccggccg
      781 gggtgggcgt cgtgatggcg tggcagcgtc cgtgggagga gccgccgggt ggtgttccgt
      841 cggcgccggt gatcgagcac ggcaggaatc gcgttgtcgt ggtgtgccca tggtgcgctg
      901 atcgccatca gcacacgaag gaatgcgccg ggagccgtga ggtagttgcg cattgcggcc
      961 gttggggtga cttccggtcg tacagtgttc cggccgcgcc gggtggccgg cggtgagcgg
     1021 ggagtacacg ccgccgacgg tcccgacgct gggcgacgac ggtttgaagg ttggtgctgg
     1081 tcatggccag ggtcggcggg ctgatcggtg gccgacgcat cgcggtcggg cgcgcgacaa
     1141 ggcggcccgg catcgtgcgc gccgcgagtt ttggaggaag gtacgcgcga ctggccgtgt
     1201 cccggcgtgg ctgcccgact tcgtgttcga cgcgcccggc tgggcgggat ccgacgggtg
     1261 accgcgttgt ggccgcgcat gcccggcgag ccgcctgcgg atcggtacgt ggtgcgggcc
     1321 ggcgtgctcc ccgacggttg ggaggggtgc cctgttttcg cgcgccctgc cgatgtggct
     1381 gaggcgttgc ggtgccgtga cgcgacgtcg gctgcccgga tggccgggtg tccggaagac
     1441 gaggccctgt tcgtcgcgga agatcccaac aatcgccacg attcgatgcg cttcaccgac
     1501 cctggccggc tggtgctccg ccgggtgata agccgcgact gggcgttgcg tgcggcggcg
     1561 aggttccggc cggtggtcgg cgcggcattc gactacctgg cccttcgggg tgaggtggat
     1621 gcgtgcgtgg gggatgacct gttgccgcgg ttgattcgct gggtgatatt cgcaccgggt
     1681 gttgacagtt gggctgatgt ggccgcgcaa gtgccggtgc gtcgcgctga gggcgcccag
     1741 ggtgatgact tagtgtcgcg ggtgatccgg ttcgcgccgc aactgtccga ccaggaggtg
     1801 ttcgtgctgt cggcgtcgtc gttcggggtg gccggcggcg cggtcggctt catcggcacc
     1861 ctgttcacca gcacgctagc cttcctgggc gctgacaccg acgtgcagcg cagattacgc
     1921 ggaaacccct ccgatatacc ggttttcgtg gatgagatgg cgcggttgaa gacgacgaca
     1981 cccctggtga gtcgtcacgt cacagcggac acccaaatcg gcggggtgga cctgccgaag
     2041 ggttccgctt ttatcgtggc gaccggcgag gccagcgtcg gaggcgagaa tggcgagcgg
     2101 gtgaatttga gcccgcgcag gcattggggt ttttccgccg gcccgtatcg ttgcaccggt
     2161 attcacttgg tgcacgcgac gatccaggtc gccgtcgagg aattcctggc gcggataccc
     2221 gaattccgat tgtgcgatga gcgtgttgtg gccacccgcc acccgtacgg ccacactcgc
     2281 ttcaattcgg cgccgctcgc gtgggacggt tgaccgtctg gtcgtgagca ccgctcgtag
     2341 ctcgaggcca gtaacgcgcg ttgtcgtacg cggaggttac tgtctgcccc tatgactgaa
     2401 gatgcgttgt catggctggc cgctctacct cgagcggtcc agaccgcatt gttagctaat
     2461 ccgagcggcg acgtgccgcc agacatcatc acgcggatgc cgagccttgt cgtgaagacg
     2521 tactggacga gcaatccaga cgccggccgt tgggaactgc accgccagta cgcgaccgct
     2581 gcgacggacg gtcgggcgcg gctggatagg tggtggaagt ccctaccact agccacccgt
     2641 gacgctttgg tggagcaccg cgatgggttg gtgcccaatg agcaccgcgc gtacgtcgct
     2701 gatctgggcg ccctcggtgt cgcggtctat gtcggcgcct cgtctgcggg ggagttttca
     2761 ttgcatccac tggtcagcgc gtacctagac ttgcacgccg aatcggcgta gagaaacgcc
     2821 cggcccgggc cgggcgggca accaattccc cctacttcag cccgagtgct tgcagcgctg
     2881 cgcgacgatc ctcgcccgac atcagcgcga atacccatcc aaaatcgtgg tggttcgctt
     2941 gccacagcgc gattgcgttc ttgacggccg cattcatggt tggtcctctc gcgatcggta
     3001 agcagtgggt tagtttgccg ctgtgccgtg tcgtcaacgt ggctgttggt agccgcgttc
     3061 cccagtgcga gtaagcattt ggtgtggatt tcaggtggcg ccggtgggcg tacgcccttt
     3121 tctacgccca ccagggtttc ccaacggtgg ttaacggttg ccagaaaagt gatttgagct
     3181 gcgaaaacta gcaaccgtta accaacagaa acgcgggaaa catgtctggg ggtcaagtgg
     3241 tcgcaggttc aaatcctgtc agcccgacca gagtctaagc agctcggagc atgtttcctg
     3301 ccgcagccag caaagcctgt gtgatcggtg tgcgatctga aatgtcagct cgatggttgt
     3361 tgaactcgat ggcccgtcgc ttcggtggcc ggacatcact atgcgtcggc tgctgtttcc
     3421 cgccgcccag ctcgatcctg ccgcgtcccc gcgcgtgccg gtgacggatc gtagaaccgt
     3481 gccgcccgcc ggctcggcgt cgcgctcatc ttggccgtcg aggaggccga ggagatggcc
     3541 ggctacgacc agatcaccat ttccatgacc tgaaccacag gcgccggtgt cggcccgtca
     3601 tccttcgggg tggcgggccg aaccgggttg aggaccaccg gcgtcgtcgc caccggcctc
     3661 gtcgttggcc gccgccgatg ttagtcggtg ctaggg
//
LOCUS       DASSGQ010000154         7096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_346113,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000154 DASSGQ010000000
VERSION     DASSGQ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346113"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="VFI55_06935"
     CDS             complement(<1..166)
                     /locus_tag="VFI55_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HET7073801.1"
                     /translation="MTGVPDVDMLNAFNKNIIKEFRANDGKVTGQFANANLLLLTTTG
                     AKSGQQRVSPL"
     gene            complement(218..2029)
                     /gene="aspS"
                     /locus_tag="VFI55_06940"
     CDS             complement(218..2029)
                     /gene="aspS"
                     /locus_tag="VFI55_06940"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET7073802.1"
                     /translation="MGSHSNRRKGVFVLRSHAAGALRNRDAGQQVTLAGWVARRRDHG
                     GVIFIDLRDASGVAQVVFRSPDVLAQAHRLRAEFCVAVSGVVEIRPEGNANPEIATGD
                     IEVNATSLTVLGESAPLPFQLDEPAGEELRLKYRYLDLRRDGPAAAIRLRSKVNAAAR
                     SVLARHDFVEIETPTITRSTPEGARDFLVPARLHPGSFYALPQSPQLFKQLLMVAGME
                     RYYQIARCYRDEDFRADRQPEFTQLDMELSFVDVEDVIAISEEILSALWELIGYQIPT
                     PIPRISYADAMRRFGSDKPDMRFGLELVECTEFFSDTTFRVFQAPYVGAVVMPGGASQ
                     PRRTLDGWQEWAKQRGHRGLAYVLVGDDGTLAGPVAKNLTDAERDGLAAHVGASPGDC
                     IFFSAGPVKSSRALLGAARGEIANRLGLIDPDAWAFVWVVDPPLFEPAEEATAAGDVA
                     VGSGAWTAVHHAFTSPKPEYQDAVDSDPGNVLADAYDIVCNGNEIGGGSIRIHRRDIQ
                     ERVFAVMGLNKAEAEEKFGFLLEAFMFGAPPHGGIAFGWDRINALLSGVDSIREVIAF
                     PKTGGGVDPLTEAPAPITAQQRKESGIDAKPKGVQQA"
     gene            2018..2962
                     /locus_tag="VFI55_06945"
     CDS             2018..2962
                     /locus_tag="VFI55_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015385292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET7073803.1"
                     /translation="MTAQSKHPPAQGNFGRIPTNIALVGPGAVGTTVAALLYKAGHPV
                     LVCGRTARPPSHSIELRPDGADPIVVPGPVHTDPDDVTGPVDLVVLAVKATQNDAAAG
                     WLGRLCDAHTVVVVLQNGVEQVEQVKPHCPASQVVPAIVWYSAETQPEGWVRLRTEAR
                     LVLPSGPPAEWVAGLLRGAGCQVECDPDFLTAAWRKLLVNALAGFMALTGRRAGMFRR
                     DDVAALSRRYVAECLAVAQAEGARLPDHTVDELVDLFRDAPEDLSTSMLADRENHRRL
                     EWDIRNGVIVRKARAHNLATPVSDVVVPLLAATSDGPG"
     gene            3032..3916
                     /locus_tag="VFI55_06950"
     CDS             3032..3916
                     /locus_tag="VFI55_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HET7073804.1"
                     /translation="MTFNEGMQIDTSTASSSGGGIGGLPIAIGGGLGGLLILIVALFF
                     GVDPGGIVSQQPMDTRDDVSPGFDLSQCKTGADANKYVQCRVVATGNSVDAVWHQLMP
                     SYTRPHVRLFSGQVDTGCGPATTAVGPFYCPVDQTAYFDTDFFQELVDKFGSSGGPFA
                     QEYVVAHEYGHHVQQLQGNLGRSQQGAQGAGGNGVRTELQADCYAGIWAHYASTVKQE
                     STGVPYLEPLSDKDIADALSAAASVGDDRIQKETSGRVNPESWTHGSAAQRQKWFTTG
                     YQTGDPNKCDTFAATNLG"
     gene            complement(3942..4370)
                     /locus_tag="VFI55_06955"
     CDS             complement(3942..4370)
                     /locus_tag="VFI55_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073805.1"
                     /translation="MTSVRKVTSAVVGAALVVLGPATAHADPAGHQVTYTITSTGNLT
                     GNVRYMNSDPPSQAAFDANSSQYLNSVQTPFTGGQPLVYTTTLANPNQWAFVAASGAC
                     HWPDCSSGAVAELHCEIAVDGQVVVTQNATTGVTCATRPW"
     gene            4432..6033
                     /locus_tag="VFI55_06960"
     CDS             4432..6033
                     /locus_tag="VFI55_06960"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET7073806.1"
                     /translation="MSEDPAARDIGPHSPGISRRRLLTSSVASAVLGGVGVGGAALVW
                     SDHSHPRGPAVWYRPDRNGAPPVVGLHLQFGSNAGTEVVVSWHTTDAVSNPRVMLGTP
                     TSGFGQTVAAETRTYRDGKSGVEVRVNHARLTGLTPDTDYIYAAVHDGAAPEPGTVRT
                     APLGRKPLRFTSFGDQSTPALDKLPGGYGTDNIGSPAAADTTLAIERLGPLFNLVNGD
                     LCYANLALDRIRTWSDWFENNTRSARYRPWMPAAGNHENELGNGPIGYGAYQTYFALP
                     DAGSSAETRGLWYSFTAGSVRVVSLNNDDVAFQDGGNFYVHGYSGGEQKRWLAAELAA
                     ARRDPDVDWVVVCMHQTAISTADRTNGADLGIREEWLPLFDQYQVDLVVCGHEHHYER
                     SHPLRGALGTDTRTPIPVDTRGDLIDATRGTVHLVIGGGGTSAPSNGMFFPTPRCRVL
                     TGVGAFDPTIGRKAPIYVIEDAPWSAFRDRDNPYGFVAFDVDPGHPGGNTSIKATHYA
                     LSGLFGAVTVIDEFTLTKPRGDKPA"
     gene            complement(6034..7074)
                     /locus_tag="VFI55_06965"
     CDS             complement(6034..7074)
                     /locus_tag="VFI55_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2578c family radical SAM protein"
                     /protein_id="HET7073807.1"
                     /translation="MRWAGQSVAVNGVPVDDGALPGLQRIGLVRSVRAPQFEGITFHE
                     VLCKSALNKVPNAAALPFRYTVNGYRGCSHACRYCFARPTHEYLDFDCGTDFDTQVVV
                     KTNVAEVLRHELHRPSWQRETVALGTNTDPYQRAEGRYALMPGIIAALAGSGTPLSIL
                     TKGTLLRRDLPLIAEAAGQVPVSVAVSLAVGDPELHRDVEPGTPTPQARLGLIGAIRD
                     AGLDCHVMVAPVLPHLTDSAEHLDMLLGQIAAAGATGVTVFGLHLRGSTRGWFMSWLA
                     RSHPELIGRYRDLYRRGAYLPADYREMLAARAAPLIAKHRLAGEHRPVPRAATPARAS
                     TVPFSAAQLKLF"
BASE COUNT         1114 a         2429 c         2455 g         1098 t
ORIGIN      
        1 ccagtggcga cactcgctgt tggccggact tggcgcccgt cgtcgtcagc agcagcaggt
       61 tggcgttggc gaactggcca gtcaccttcc cgtcgttggc ccggaactcc ttgatgatgt
      121 tcttgttgaa cgcgttgagc atgtcaacgt caggaactcc ggtcatttgc gccgctcctc
      181 ctcattgctt cgcgctgcat cgtcgccggc gcgggggtca tgcctgctga acccccttgg
      241 gcttggcgtc tattccggac tccttgcgct gctgggcggt gatcggcgcg ggcgcctcgg
      301 tgagcgggtc gacgccgccg ccggtcttcg ggaacgcgat cacctcgcgg atggagtcga
      361 cgccggacag cagcgcgttg atccggtccc agccgaacgc gatcccgccg tgcgggggtg
      421 cgccgaacat gaacgcctcc aacaggaagc cgaacttctc ctcggcctcg gccttgttca
      481 ggcccatcac cgcgaagacc cgttcctgga tgtcgcggcg atggatacgg atcgagccgc
      541 cgccgatctc gttgccgttg cagacgatgt cgtaggcgtc ggccagcacg ttgcccgggt
      601 cgctgtcgac cgcatcctgg tactcgggct tgggagaggt gaacgcgtgg tgcacagcgg
      661 tccacgcccc cgacccgacc gccacgtcac cggcggcggt cgcctcttcg gcgggctcga
      721 acagcggcgg gtcgacgacc cagacgaagg cccacgcgtc cgggtcgatc aggccgagcc
      781 ggttcgcgat ctcgccgcgg gcggcaccca gtagcgcccg tgacgatttc accggacccg
      841 ccgagaagaa gatgcaatcc cccgggctgg cgcccacatg cgcggccagg ccgtcgcgtt
      901 cggcatcggt gaggttcttg gcgaccggac cagccagcgt gccgtcatcg ccgaccagca
      961 catacgccag tccccggtgc ccgcgctgtt tggcccattc ctgccagccg tccagggtgc
     1021 gccgcggctg cgaggccccg ccgggcatca ccacggcacc gacgtacggg gcctggaaga
     1081 cgcgaaacgt ggtgtcggag aagaactccg tgcactcgac gagctccagg ccgaacctca
     1141 tgtcgggctt gtcggagccg aaccgtcgca tggcgtcggc gtagctgatc cgcgggatcg
     1201 gggtcgggat ctgatacccg atcagctccc acagcgcgct caggatctcc tcggagatcg
     1261 cgatgacgtc ctcgacgtcg acgaagctca gctccatgtc gagctgggtg aattcgggct
     1321 ggcggtcggc gcggaagtcc tcatcgcggt agcagcgcgc gatctggtag tagcgctcca
     1381 tcccggccac catcagcagc tgcttgaaca gctgcgggct ctgcggcagc gcgtagaacg
     1441 agcccgggtg cagccgggcc ggcaccagga agtcgcgcgc cccttccggc gtcgagcggg
     1501 tgatcgtcgg cgtttcgatc tcgacgaagt cgtggcgcgc cagcaccgag cgggcggcgg
     1561 cattcacctt ggagcgcaac cggattgccg cggccgggcc gtcgcggcgc aggtcgaggt
     1621 agcggtattt cagccgcaac tcctcccccg ccggctcgtc aagctggaac ggcagcggcg
     1681 cgctctcacc gagcacggtc agcgaggtgg cgttgacctc gatgtcgccg gtggcgatct
     1741 ccgggttggc gttgccttcc gggcggatct cgacgacacc ggagacggcg acgcagaact
     1801 cggcgcgcag ccggtgcgcc tgggccagca cgtcgggaga acggaacacg acctgtgcga
     1861 cgccggaggc atcgcggaga tcgatgaaga tgacgccgcc gtggtcgcgg cggcgagcca
     1921 cccaaccggc caacgtcacc tgctggccgg cgtcgcgatt tcggagcgca ccagcggcgt
     1981 ggctgcgcag cacaaatact ccctttcgcc ggtttgaatg actgcccagt ctaaacaccc
     2041 cccggcgcag ggtaacttcg gtcgcatacc aaccaacatc gcgcttgtcg gtcccggcgc
     2101 cgtgggcacg acggtggcgg cgctgttgta caaggccggg catccggtgc tggtctgtgg
     2161 ccgcaccgct cgcccgcctt ctcacagcat cgaattgcgc cccgacggcg ccgatccgat
     2221 cgtggtgccg ggtccggtgc acaccgatcc cgacgacgtg accggcccgg tcgacctggt
     2281 ggtgttggcg gtgaaggcca cccagaacga cgccgcggcg ggctggctgg gacgcctgtg
     2341 cgacgcacac accgttgtcg tggtgctgca gaacggcgtt gagcaggtcg agcaggtcaa
     2401 gccgcattgc ccggcgtcgc aggtggtccc ggcgatcgtc tggtacagcg ccgagaccca
     2461 gccggaagga tgggtgcggc tgcgcaccga agcccggctg gtgctgccgt ccggcccccc
     2521 ggcggaatgg gtcgccggac tgctgcgcgg cgccggctgc caggtggagt gcgacccgga
     2581 cttcctcacc gcggcctggc gcaagctgct ggtcaacgcg ctggccggat tcatggcgct
     2641 gaccgggcgg cgggccggca tgttccgccg cgacgacgtc gccgcgctgt cgcgccgcta
     2701 tgtcgccgag tgcctggccg tcgcgcaggc cgagggggcc cggctgccgg accacacggt
     2761 cgatgagctc gtcgacctgt tccgcgacgc cccggaggac ttgagcacgt cgatgctggc
     2821 cgaccgggag aaccaccggc ggctggaatg ggacatccgc aatggcgtga tcgtccgcaa
     2881 ggcccgcgcc cacaacctgg cgacgccggt cagcgacgtc gtggtgccgc tgctggccgc
     2941 gaccagcgat ggtcccggtt aaggtcatcc ggccgccgct gtgcggcgtt aggctggttc
     3001 cgcctggccg atcagatttc gggagcgtgc gatgaccttc aacgagggta tgcagatcga
     3061 caccagcacc gcgtcgtcat ccggtggcgg gatcggcggc ctgccgatcg ccatcggggg
     3121 cggcctcggc gggctgctga tccttatcgt ggcgctgttc ttcggcgtcg atcccggcgg
     3181 catcgtcagc cagcagccga tggacacccg cgacgacgtg tcgcccggat tcgacctgag
     3241 ccagtgcaag accggggccg acgccaacaa gtacgtgcag tgccgcgtgg tggccaccgg
     3301 caattccgtg gacgcggtct ggcatcagct gatgccgagc tacacccgcc cgcatgtgcg
     3361 gttgttcagc ggccaggtcg acaccggctg tggaccggcc accaccgcgg tgggcccgtt
     3421 ctactgcccg gtggaccaga cggcgtattt cgacaccgac ttcttccagg agctggtcga
     3481 caaattcggt tccagcggtg gaccgttcgc gcaggagtac gtggtggcgc acgaatacgg
     3541 ccaccacgtg cagcaactgc agggcaatct gggccgctcg cagcaaggcg cgcagggcgc
     3601 cggcggcaac ggggtgcgca cggagttgca ggccgactgc tatgcgggca tctgggcgca
     3661 ctacgcgtcg acggtcaagc aggaaagcac cggggtgccc tacctggagc cgttgagcga
     3721 caaggacatc gccgatgccc tgtcggccgc ggcgtcggtg ggcgacgacc gcatccagaa
     3781 ggagaccagc gggcgcgtca accccgagtc gtggacgcac ggttcggcgg cgcaacggca
     3841 gaagtggttc accaccgggt accagaccgg tgacccgaac aagtgcgaca ccttcgccgc
     3901 caccaacctg ggctagctgc tacccgggtt cggttgaacg gctaccaggg ccgcgtcgcg
     3961 caggtcacgc ccgtggtggc gttttgggtc accaccacct ggccgtcgac ggcgatctcg
     4021 cagtgcagtt cggcaacggc ccccgagctg cagtcaggcc agtgacacgc gccgctggcg
     4081 gcgacgaaag cccactggtt cgggttcgcc agcgtggtcg tgtacaccaa cggctgcccg
     4141 ccagtgaacg gcgtctgaac ggagttcagg tactgcgacg agttggcgtc gaaggccgcc
     4201 tggctgggtg ggtcgctgtt catgtaccgg acgttgccgg tcagattgcc ggttgaggtg
     4261 atcgtgtaag tcacctggtg gccggcggga tccgcatgcg ccgtcgccgg cccgagaacc
     4321 accagggcag cccccacgac cgcgctggtc acttttcgca cgctggtcat gtcgaaaacg
     4381 ctaccggatt cgttaccgac ggttcggccc aaactccggt atgaagcttg catgagcgag
     4441 gatcccgccg cacgggacat cgggccgcac agccccggca tcagccgccg gaggttgttg
     4501 acgtcaagcg tggcctccgc ggttctcggc ggcgtcgggg tgggcggggc cgcgctggtg
     4561 tggtccgacc actcgcatcc ccgcggcccg gccgtttggt accggccgga tcgcaacggg
     4621 gcgccgccgg tggtcggttt gcacctgcaa ttcggcagca acgccggcac cgaggtggtg
     4681 gtgtcctggc acaccaccga cgccgtcagc aatccgcggg tcatgctggg cacgccgacg
     4741 tcgggcttcg ggcagaccgt agcggccgaa acccgcacct accgggacgg caaatccggc
     4801 gtcgaggttc gcgtcaacca cgctcgcctg accggtctga ccccggacac cgactacatc
     4861 tacgccgcgg tgcacgacgg cgccgccccg gagccgggga cggtgcgaac cgcgccgcta
     4921 gggcgaaaac cgctgcgctt caccagcttc ggcgaccagt ccactccggc actggacaag
     4981 ttgcccggcg gttatggcac cgacaacatc ggctcgcccg ccgcggccga caccacgctg
     5041 gcgatcgaac gcctcggccc cctgttcaac ctggtcaacg gcgacctgtg ctacgccaac
     5101 ctcgccctgg accggatccg cacctggtcg gactggttcg agaacaacac ccgctcggcg
     5161 cgctaccggc cgtggatgcc ggcggcgggt aatcacgaga acgaattggg caacgggcca
     5221 atcggttacg gcgcgtatca gacgtacttc gcgttgcccg acgccggatc cagcgcggaa
     5281 acccgcgggc tgtggtactc gttcaccgcc ggctcggtgc gggtggtcag cctcaacaac
     5341 gacgacgtcg ccttccagga cggcggcaac ttctacgtac acggctactc gggcggcgag
     5401 cagaagcgct ggctggcagc cgaactcgcc gccgcacggc gcgacccgga cgtggactgg
     5461 gtggtcgtct gcatgcatca gacggcgatc tcgaccgccg accgcaccaa cggcgccgac
     5521 ctcggcatcc gcgaggaatg gctgccgttg ttcgatcagt accaggtcga tttggtggtc
     5581 tgcggccacg aacaccacta cgaacggtcg catccgctgc gcggggcact gggcaccgat
     5641 acccggacac cgatccccgt cgacacccgc ggcgatctca tcgatgcgac acgcggaacg
     5701 gtgcatctcg tcatcggtgg gggcggcacc tcggcgccgt ccaacgggat gttcttcccc
     5761 accccgcgct gccgggtgtt gaccggggtc ggcgcgttcg atccgacgat cgggcgcaag
     5821 gcaccgatct acgtgatcga ggacgctccg tggtcggcat tccgcgaccg cgacaaccct
     5881 tacggcttcg tggctttcga cgtcgacccg ggccaccccg gcggcaacac ctccatcaag
     5941 gcgacgcatt atgcgctgag cgggctgttc ggcgcggtca ccgtcatcga tgagttcacc
     6001 ctgaccaagc cccgcgggga caagcccgcc tagctaaaac agtttcagct gggccgccga
     6061 aaaaggcacg gtcgacgccc gcgccggtgt ggcggcccgc gggaccgggc gatgctcacc
     6121 ggccaggcga tgtttcgcga tcagcggcgc ggcgcgtgcg gccagcatct cgcggtagtc
     6181 ggccggcagg tacgcgccgc gccggtacag gtcgcggtaa cggccgatca gttcggggtg
     6241 cgagcgggcc agccaggaca tgaaccagcc gcgggtcgag ccgcgcagat gcaggccgaa
     6301 gaccgtcact ccggtggccc ccgcagccgc gatctggccc agcagcatgt cgaggtgctc
     6361 ggcggagtcg gtgaggtgcg gcagcaccgg cgccaccatc acgtgacagt ccaggccggc
     6421 gtcgcgaatt gcgccgatca gacccagccg cgcctgcggt gtcggggtgc ccggctcgac
     6481 gtcgcggtgc agctccgggt cgccgacggc cagcgacacc gcgaccgaca ccggaacctg
     6541 ccccgccgct tcggcgatca gcggcaggtc gcggcgcagc agtgtgccct tggtgaggat
     6601 ggacagcggc gtaccggatc cggcgagggc ggcaatgatg cccggcatca gcgcgtagcg
     6661 gccctcggcg cgctggtagg gatcggtgtt ggtgcccagc gcgacggttt cacgctgcca
     6721 cgacggccgg tgcagctcgt ggcgcagcac ctcggcgacg ttggtcttga ccaccacctg
     6781 ggtgtcgaag tcggtgccgc agtcgaagtc caggtattcg tgggtgggac gggcgaagca
     6841 gtagcgacat gcgtgcgagc aaccgcggta gccgttgacg gtgtaccgga acggcagtgc
     6901 cgccgcgttc ggcaccttgt tgagcgccga cttgcacagc acctcgtgaa aggtgattcc
     6961 ctcgaactgc ggggcgcgca cgctccggac gaggccgatc cgctgcagcc ccggcagcgc
     7021 cccgtcatcg acgggcaccc cgttgactgc cacactctgg ccggcccagc gcatagttcc
     7081 attcgaacaa tagttc
//
LOCUS       DASSGQ010000155         8448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1500146,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000155 DASSGQ010000000
VERSION     DASSGQ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8448
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500146"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..786)
                     /locus_tag="VFI55_06970"
     CDS             complement(82..786)
                     /locus_tag="VFI55_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7073808.1"
                     /translation="MKTAVVTGGASGIGRAIAQRLRSNGFQVAVIDLSPTDDGFGYVA
                     DVTQRAQVDTAVSAIRNAFGPILVLVNAAGVEGFTKFLGMSFDEWSKVIDVNLNGVFH
                     TIQAVLPDMVEAGWGRIVNISSSSTHSGQPFMAHYVAAKSAVNGLTKSLALEYGPSGI
                     TVNAIPPGFVDTPMLRGAERRGLLGGTVEDHILRTPVRRVGRPEDIAGACAFFVSEEA
                     GYITGQILGVNGGRNT"
     gene            929..1549
                     /locus_tag="VFI55_06975"
     CDS             929..1549
                     /locus_tag="VFI55_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7073809.1"
                     /translation="MPASERPKARNSTTRDMLLDATTQIMVDEGYAAATSRRVAAKAG
                     VKPALVHYYFPTMDELYLAVFRRGATVYLERQQKALASDRPLHAFWDTLTGPKDTRLL
                     LEFMGLANHRKEIRAEIAAWSERWRDQQITALHFIVREHDIDTSEFPPAAIAVVIASI
                     GRSLILEQDLGAARGHDEAVALVNRFLDRFEMPSPTARRERDGGPG"
     gene            complement(1546..2748)
                     /locus_tag="VFI55_06980"
     CDS             complement(1546..2748)
                     /locus_tag="VFI55_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7073810.1"
                     /translation="MTVTNDTDVYYDPYDVGINADPYPTYARLRDEAPIYYNEHYDFW
                     AISRHSDVEHALANWEVFSSRRSDILELLQSKFDMPGGVMMFQDPPEHSMLRGLMSRV
                     FTPRRMAAIEDQIRQYCIRCLDPLVGSGGFDIITELASMMPMRVIGMLLGIPESEQIS
                     VRDANDANLRTKPGAPLRVADADSIADGRIYADYVEWRSKNPSDDLMTTLLNVEFDDE
                     RGVRRKLTRKEVLHYTQVVAGAGNETTGRLIGWLAKVLAEHPDQRREVYEDRSLLNRA
                     VDETLRFEPTGPHVARWMATDFECHGTTVPAGGAMLLLFGAANRDPRRYTDPDTFNIH
                     RENMSHLTFGKGLHYCLGANLARLEGRVALDELLNRWPVWDIDYGTAQLASTSTVRGW
                     ERLRVVLP"
     gene            complement(2854..3447)
                     /locus_tag="VFI55_06985"
     CDS             complement(2854..3447)
                     /locus_tag="VFI55_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7073811.1"
                     /translation="MTEPATRAPGGRGARERIERAAARLFYRNGIHATGVELIAQQAN
                     VSKRTLYQHFASKKDLVDNYLRGIDSRGGPPVERRLNDTALSARERLLGIFEMSQSEV
                     LRGCPFHNAAVESAGTLTSTDEIVRAHKLEFTRRLVAVAHEAGAADPHLLGQQLAVLF
                     EGATAMATSLNDTAPVVHARAAAAALIDAELATSRNS"
     gene            3544..4134
                     /locus_tag="VFI55_06990"
     CDS             3544..4134
                     /locus_tag="VFI55_06990"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET7073812.1"
                     /translation="MPSHDVPRGALDTADTVGWGTEQSRLIAWRDPISTQATAASMSG
                     LAYWRAVADGHLPPPPIGELLQMRVVDVANGRVTFSCTPDASMYNTLGMVHGGAVCTL
                     LDTVTGCAVHTTLPEGVGYTSVEIKVNYLRAVTMASGPLTAVGTVVKAGSRIGFTEGK
                     VTDVSGALVATATSTLLVFDLAGPAKHSGTNGNRVG"
     gene            4188..4949
                     /locus_tag="VFI55_06995"
     CDS             4188..4949
                     /locus_tag="VFI55_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7073813.1"
                     /translation="MPSVLITGANRGIGRAIATEFASRGHRVVATARDPRTLADLDVS
                     QRLALDVTDDASVTAAVAAAGEIDILIANAGVIVYAAVEATPLPELQRLLNLNTVGAV
                     RVAQVVLPQMRARGDGKLLFMSSVLGRVVLPPSAAYAATKWALEALVEALAIEVAPFG
                     VQAALLEPGAVSSGALDDVTTYTLPDDPYAAILKAGGPRAGMITPEQVATEVVDAAEK
                     PQLPLRVAIGDGARALLAARKSAPDDVPFVAAGRS"
     gene            5075..6439
                     /gene="lysA"
                     /locus_tag="VFI55_07000"
     CDS             5075..6439
                     /gene="lysA"
                     /locus_tag="VFI55_07000"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET7073814.1"
                     /translation="MTLFEVLPSARAEVHPHLDRAIWPLSAGVDEGGRLCVGGVAATE
                     LAAKFGTPTYVIDEEDFRARIRCFRAALPGVELVYAGKALLSTAVAGWVATEGAGVDV
                     CSVGELATALTGDVPPSKIILNGNAKTARELDDAVAAGVGRVVIDTPNEIALLAGRVR
                     RRQRVLIRVIPDIDPSAVRTGLLDQKFGFALADGQAFSAVKRVLGEPWLDLVGLHCQL
                     GSQLSDAALYGDAIRQMVAVMAEVRDRHGVVLTELNLGSGHAVPYCSGDPELDPRALG
                     AAVESALESACAAHRYPRPQIVVEPGRAIAARAGITLYRVVAVKRQSDGHTVVAVDGG
                     MSDNPRAVLCGTQYTVALANRHLPAATTRVTVVGRHCEPGDEIARDVELPADVHPGDL
                     LAVAGTGAYHYSMGSSCSLVGRPPLVAVAGGKSRELIRRETLADLLARDHGWSGRSHR
                     GECR"
     gene            6436..7242
                     /locus_tag="VFI55_07005"
     CDS             6436..7242
                     /locus_tag="VFI55_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid-like protein"
                     /protein_id="HET7073815.1"
                     /translation="MTAVAVREYTNRWSAVAARTLSGAASVRITAAYAIVLAAVSLRL
                     TALGPRARDEVVSRMSTNLHNLSHGHLSTLIGSAFVDGGGTIYFWLPELVCLLALGEL
                     IWRSKGLVVAFAVGHIGATLIVAVGLTVALEAGWLPFSIAHASDVGMSYGAVSVLGAL
                     TASIPSRWRPAWVGWWLGVAVTAAAISADFTAVGHVLALLLGMGLSFRLPMIAHWTLL
                     RMLLLLVGSAFGYFVMSGSSVAATAGGLAGALIAVVAGLRRARRVSRTAP"
     gene            7309..8340
                     /locus_tag="VFI55_07010"
     CDS             7309..8340
                     /locus_tag="VFI55_07010"
                     /inference="COORDINATES: protein motif:HMM:NF045184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HET7073816.1"
                     /translation="MNDDVLVVDDFLPGALYQDLAAHTSRQPLIYGSRSNFKTDPHGH
                     WSLDFVAAGSQNLADVSVDLEADEAAAPIWQAWRFLQSTQLSGHVLIRCYVNGYTYGT
                     DGYFHSDSERSDEHTVVVYLNDYWEPDWAGETVFLGEDDDVIKAVLPRRNRAVIFPST
                     LQHAGRAVSRKCTVLRKTLIFKTRKTRSDDFEKLSVFLRDAGARQCAHRRGTLHDHLV
                     RTFSLLEAKQLDPAVCLGGGLHAIYGTNAFRGAVLGSDARTHVGREFGERAEELAHLF
                     SRIDRPKCLESPPHLGADTATVELADGGSLELRRTVFDDLRKIECANLADQGTLSRHR
                     ALSALWETL"
BASE COUNT         1409 a         2771 c         2784 g         1484 t
ORIGIN      
        1 gaccgtgata gctgaatcac cctgcgctgc agtccatttg ccgtcgataa agagttgatc
       61 ccgctggtac atctggtcct ctcaggtgtt gcgaccaccg ttgacgccga gtatctgtcc
      121 ggtgatgtag ccggcttcct cggagacaaa gaacgcgcag gccccagcga tgtcctccgg
      181 tcggcccact cggcgcaccg gggtccgcag gatgtgatcc tccacggtgc cacccagcag
      241 cccgcgccgc tcggcgccgc gcagcatcgg ggtgtcgacg aatcccggcg gtatcgcgtt
      301 gacggtgatc cccgacggac cgtattccag ggccagcgat ttggtcaggc cgttcaccgc
      361 ggacttggcc gcgacatagt gggccatgaa aggctgcccg gagtgagtgc tggacgacga
      421 gatgttgacg atgcgtcccc atccggcctc gaccatgtcg ggcaggacgg cctgaatggt
      481 gtggaaaacg ccgttcaggt tgacgtcgat caccttcgac cattcgtcga aggacatgcc
      541 caagaacttc gtgaagcctt ccaccccggc cgcgttgacc aagaccagga tgggaccgaa
      601 cgcgttgcgg atcgccgaca cggcggtatc cacctgagca cgttgggtga cgtcagcgac
      661 atagccgaag ccgtcgtcgg tgggtgacag atcgatgacg gccacctgaa atccgttgct
      721 gcgcaatcgc tgtgcgatgg cgcgcccaat gcctgacgca ccgccggtca cgaccgccgt
      781 cttcacgccc acctgcctcc actgccctga gcgaccgccc aaacggctag ccccacatgc
      841 ctatcaccgt gtgtccccca gggtcaagcg ggaatgcggg catgggaact gcccgtgtta
      901 ggcgatcgct cagatagcct gttacaccat gcccgcatcc gaacgaccga aggcacggaa
      961 ctccaccact cgcgacatgc tgctggacgc aacgactcag atcatggtcg acgagggtta
     1021 cgccgcggcc acgtcgcgca gggtggcggc caaagccggt gtcaaaccgg ccttggtgca
     1081 ctactacttt cccacgatgg acgagttgta cctggctgtc ttccgccgcg gcgccaccgt
     1141 ctatctggag cgtcaacaga aagcgttggc ctctgaccga ccgctgcacg ctttctggga
     1201 tacgctcacc ggacccaagg acacccgact gctgttggag ttcatggggc ttgccaacca
     1261 ccgcaaggag atccgggccg agatcgcagc ctggtcggag cggtggcgcg accagcagat
     1321 caccgcactg cacttcatcg ttcgcgaaca cgacatcgac acaagcgaat tcccgcccgc
     1381 ggccatcgcg gtcgtcatcg cctcgatcgg ccggagcctc attttggagc aggatctggg
     1441 tgccgcacgc ggccacgacg aggccgtcgc gctggtcaac cgattcctgg accggttcga
     1501 gatgccgtca ccgacggctc gccgcgaacg cgacgggggc cccggctagg gcagcacgac
     1561 ccgcagccgt tcccagccgc ggaccgtaga cgtcgaggcc aattgcgccg tgccgtaatc
     1621 gatgtcccac acgggccagc ggttgagcag ctcgtccagt gcgacgcggc cctcgagtcg
     1681 tgccaggttt gctccaaggc agtaatgcag gcctttcccg aaagtgaggt gcgacatgtt
     1741 ttcccggtgg atattgaacg tgtccggatc ggtgtaccgg cgggggtctc tgttggccgc
     1801 accaaacagc aggagcatgg cgccgccggc cggaacggtg gtcccgtggc actcgaaatc
     1861 cgttgccatc caacgggcga cgtggggccc cgtcggttcg aacctgagtg tctcgtcgac
     1921 ggcgcggttc agcagcgacc gatcttcgta gacttcccgg cgctggtcgg gatgctcggc
     1981 gaggaccttg gccagccaac cgatcagccg gccggtcgtc tcgttgcccg cgccggcgac
     2041 cacctgggtg taatgaagca cctctttgcg ggtcagcttc cggcgcactc cccgctcatc
     2101 gtcgaactcg acgttgagca gtgtcgtcat caggtcgtcc gaggggttct tggatcgcca
     2161 ctcgacgtaa tcggcgtaga tccggccgtc cgcgatggag tccgcgtcgg cgacccggag
     2221 cggtgcgcct ggcttggtgc gcaggttggc gtcgttggcg tcgcgcaccg agatctgttc
     2281 ggattcaggg attcccagca gcatcccgat cacccgcatc ggcatcatgg acgccagctc
     2341 ggtaatgatg tcgaacccac cggacccgac gagcgggtcc aggcaccgga tgcagtactg
     2401 ccggatctgg tcctcgatgg cggccatccg ccgcggcgtg aacacccgcg acatcaggcc
     2461 gcgcagcatc gagtgttcgg gcggatcctg gaacatcatg accccgccgg gcatgtcgaa
     2521 cttcgactga agcagctcga ggatgtcgct gcgacggctg gagaacactt cccaattggc
     2581 caacgcgtgt tcgacatcgg aatgccttga tatcgcccag aagtcgtagt gttcgttgta
     2641 gtagatcggt gcctcgtcgc gcagccgcgc gtaagtcggg tacgggtcgg cgttgattcc
     2701 gacgtcgtag gggtcgtaat agacgtccgt gtcgtttgtg acggtcatcg atgcttccct
     2761 tgcttcacac agctgtcgca acctcgaatc ccaattttgg gcgatcgccc aaaccgtgac
     2821 gctgccatgt ctagcagcac cgcgtcgagc tcgtcaagag ttcctggagg tggccaactc
     2881 ggcatcgatc agcgccgccg cggcggcacg ggcatgcacc acgggcgcgg tgtcgttcag
     2941 cgaagtcgcc atcgcggtcg cgccttcgaa cagcacggcc agttgctggc ccagcaggtg
     3001 cgggtcggcc gcgccggcct cgtgcgcgac ggcgaccagg cggcgggtga attccagctt
     3061 gtgggctcgc acgatctcgt ccgtgctcgt cagagtcccg gcggattcca cggccgcatt
     3121 gtgaaaggga cagccgcgca ggacttctga ttgggacatt tcgaaaatcc ccaacagcct
     3181 ctcgcgggca gacagtgcgg tgtcattcag gcgcctttcg acgggcgggc cgccgcggga
     3241 atcgatgccg cgcaggtagt tgtccaccag atccttcttg ctggcgaagt gctggtagag
     3301 cgtgcgcttg gacacgttgg cctgttgagc gatgagttcg acgccggtcg cgtggattcc
     3361 gttgcggtag aagagccggg ccgcggctcg ctcgatacgc tcccgtgcac cccgtccgcc
     3421 gggtgcacgt gtcgccggct ccgtcatgcg cccaatatac accgttcggt ttacagccgc
     3481 cgaaagtggc gctatgttgg gatggacaag taaaccgctc ggtttccaag gagctgttga
     3541 ccgatgcctt ctcatgacgt tccgcgtggc gccctcgata ccgccgacac agtgggttgg
     3601 ggcacagaac agtcgcgatt gatcgcctgg cgcgatccga tcagcacgca ggccaccgcc
     3661 gcatcgatgt ctggtctcgc gtattggcgg gcggtcgccg atggccactt gccaccgcca
     3721 ccgatcggcg agctgctgca gatgcgcgtc gtggacgtgg cgaacggccg cgtcaccttc
     3781 tcctgcaccc ccgacgcgtc catgtacaac acgctcggca tggttcatgg cggcgcggtg
     3841 tgcaccctgc tggacaccgt caccggctgt gcggtgcaca ccaccctgcc cgaaggcgtc
     3901 ggctacacgt cggtcgaaat caaggtcaat tacctcaggg cggtcacgat ggccagcggg
     3961 ccgctgacgg cggtcggcac cgtggtgaaa gcgggatcgc ggatcggatt taccgaggga
     4021 aaggtcaccg acgtctcggg agcactggtc gccaccgcga cgagcacgtt gttggtcttc
     4081 gacttagccg gtcccgcaaa gcattccggc accaacggaa atcgcgtcgg ctgagaggga
     4141 ggaaatgcga tgcgacgcga agccaaccag cgaaggagca caacgcaatg ccatccgtcc
     4201 tgatcaccgg agccaatcgc ggtatcggcc gcgccatcgc caccgagttc gccagtcggg
     4261 ggcatcgtgt cgtggccacc gcgcgtgacc cgcgcacact ggccgacctg gatgtcagcc
     4321 aacggctggc cctcgacgtg accgatgacg cgagcgtcac cgcggcggtc gcggccgccg
     4381 gtgagatcga catcctgatc gccaacgccg gggtcatcgt ctatgccgcc gtcgaggcga
     4441 cgccattgcc ggaactgcag cgcctgctca acctcaacac cgtcggcgcc gtccgcgtcg
     4501 cccaagtggt gctgccacag atgcgcgccc gcggcgatgg aaagctgctg ttcatgtcca
     4561 gcgtcctcgg ccgggtcgtc ctgccgccca gcgcggccta tgccgcgacg aaatgggcgt
     4621 tggaggcgct ggtcgaagcc ttggccatcg aggtcgcgcc cttcggggtc caggccgccc
     4681 tgctggaacc cggcgccgtg agctcggggg ccctcgatga cgtgaccacc tacaccctgc
     4741 ccgacgaccc ctacgccgcg atcctgaaag ccggcgggcc gcgcgcggga atgatcaccc
     4801 cggagcaggt cgcaaccgag gtcgtcgacg ccgcggagaa accgcagctg ccgctgcggg
     4861 ttgcgatcgg tgacggggcg cgagcgctgt tggcggcgcg taagtcggcg cccgacgacg
     4921 ttccgttcgt cgcggccggg cgcagctgat aagcacggat atttacgggc tcgtcagaaa
     4981 gccgccgtac tgaacccgtg aagatttatc tccggggtgt gaaagctttg ttaagaatgt
     5041 ggccgatccc ggttcgcgcc cgcaccgtag acctatgacg ctgttcgagg tgctgccatc
     5101 ggcgcgcgcc gaggtgcacc cgcacctcga ccgtgcgatc tggccgctga gcgccggcgt
     5161 cgacgaagga ggccggctgt gcgtcggcgg cgtggccgcc actgagctgg ccgccaagtt
     5221 cggcaccccg acctacgtca tcgacgaaga ggactttcgg gcccgcatcc gctgttttcg
     5281 cgccgcgctt cccggtgtcg aactggtcta cgccgggaag gcgctgctga gcacggccgt
     5341 ggccgggtgg gtcgccaccg aaggtgccgg ggtggacgtg tgctcggtgg gcgagctggc
     5401 caccgccctg accggcgacg tgccgccgtc gaagatcatc ctgaacggca atgccaagac
     5461 ggcgcgcgaa ttggacgacg cggtcgcggc cggggtggga cgcgtcgtga tcgacacgcc
     5521 gaacgagatc gcgctactcg ccgggcgggt gcgccgccgt caacgcgtgc tcatccgggt
     5581 gatccccgac atcgacccga gcgcggtccg taccggattg ctcgaccaga aattcggctt
     5641 tgccctggcg gacggccagg cgttcagtgc ggtcaaacgc gtcctgggtg agccgtggct
     5701 ggacttggtc gggctgcact gccaactggg gtcgcagctc agtgacgccg cgttgtacgg
     5761 cgatgcgatc cggcaaatgg tggccgtgat ggccgaggtt cgtgaccgcc acggggttgt
     5821 gctcaccgag ctcaacctcg gcagcggaca cgcggtccct tattgttccg gcgatcccga
     5881 actcgacccg cgggcgttgg gcgcggccgt cgaatccgca ctcgaatccg catgtgcggc
     5941 ccatcgttat ccccgcccac agatcgttgt cgagccgggc cgagcgatcg ccgcccgcgc
     6001 cgggatcacc ctgtaccggg tggtcgccgt caaacgccag tccgacgggc acaccgtcgt
     6061 cgcggtggac ggcggcatga gcgacaaccc gcgggcagtg ctgtgtggca cgcaatacac
     6121 ggtggcgctg gcgaaccggc acttgccggc cgccaccacg cgggtcacgg tggtgggccg
     6181 ccactgcgag cccggggacg agatcgcccg cgacgtcgag ctgcccgccg acgtccaccc
     6241 tggcgatctg ctggcggtgg ccggtaccgg cgcctaccac tactcgatgg gatcgagctg
     6301 cagcttggtg ggacgcccgc cgctggtcgc cgtggccggc ggcaagtcgc gtgaactgat
     6361 ccggcgggag accctcgccg acctgctggc ccgcgaccac ggctggtccg gacgctcaca
     6421 ccggggcgaa tgccgatgac tgccgtggcc gttcgcgagt acaccaaccg ttggtcagcg
     6481 gtggctgcgc ggacgttgtc cggcgcggca tcggtgcgta tcacggcggc gtacgccatc
     6541 gtcctggccg cggtctcgct caggctgacg gcgctcggcc cgcgcgcccg cgacgaagtc
     6601 gtgagccgca tgagcaccaa cttgcacaat ctgtcgcacg gccacctcag cacgctgatc
     6661 ggcagcgcgt ttgtcgacgg cggcggcact atctacttct ggctgccgga actggtgtgc
     6721 ctgctggcgc taggtgaatt gatctggcgc agtaagggtt tggtcgttgc gttcgccgtc
     6781 ggtcatatcg gtgccacgtt gatcgtcgcg gtggggttga ccgtcgcgct ggaggcgggg
     6841 tggttgccgt tctccattgc gcacgccagc gacgtcggaa tgagttacgg cgcggtgagc
     6901 gttctgggcg cgctgaccgc gtcgataccg tcacgctggc gcccggcgtg ggtcgggtgg
     6961 tggcttggcg tggcagtgac ggccgcggcg ataagcgcgg acttcaccgc cgtcggacac
     7021 gtcctggcgc tgctgctggg catgggtctg tctttccgac tccccatgat cgcgcactgg
     7081 acactcctac gaatgcttct gctcctcgtt ggcagcgcgt tcggctactt cgtgatgtcg
     7141 gggtcatcgg tggcggcgac ggccggcggg ctggccgggg ctctgatcgc ggttgtcgcc
     7201 gggttgcgac gcgcgcggcg cgtgtcccga acggcgccat agcaaccagg cgggtcggct
     7261 acgctggcca ccactggggg cgaacgcgaa agggaccacc gcgaaacggt gaacgacgac
     7321 gtcttggtgg tcgacgactt cctgccgggc gccctgtatc aggatctcgc cgctcacacc
     7381 tcgcgacaac ccctgatcta cgggtcacgg tccaacttca agacggatcc gcacggacac
     7441 tggagcctgg acttcgtcgc ggccggatcg cagaacctcg cggacgtgtc cgtcgacctg
     7501 gaagccgacg aagcggcagc cccgatttgg caggcctggc ggttcctgca aagcacgcaa
     7561 ttatccggcc acgtcctgat ccgctgctac gtcaacggat acacctacgg cacggacggc
     7621 tactttcaca gcgattccga acgctcggat gagcacaccg tcgtcgtcta cctgaacgac
     7681 tactgggaac cggattgggc aggagagacc gtcttcctgg gggaggacga cgacgtcatc
     7741 aaagcggtgc tgccgcggcg caatcgtgcg gtcatttttc cgtcgacctt gcagcacgcc
     7801 ggccgggcgg tgtcgcgcaa gtgcacggtc ctgcgaaaaa cactgatctt caagaccaga
     7861 aagacccgca gcgacgactt cgagaaactc agcgtctttc tgcgcgacgc gggtgcccgt
     7921 cagtgcgcac accgtcgtgg cacgcttcat gatcacctgg tcagaacgtt ttcgttgttg
     7981 gaagccaagc aactcgaccc cgccgtttgc ctcgggggtg ggttgcacgc gatatatgga
     8041 accaacgcct tccgcggggc ggtcctcggt agtgacgcca ggacacacgt aggccgcgag
     8101 ttcggcgagc gcgccgagga actcgctcat ctcttttcca ggatcgaccg tccgaaatgt
     8161 ctcgagtcac caccgcacct cggagctgac accgccacag tcgaacttgc tgatggcgga
     8221 tcgctcgaac tgcgccggac cgtgttcgac gacttgcgga agatcgaatg cgccaacctg
     8281 gccgatcagg gaaccctctc gcgacaccgg gcactcagtg cgttgtggga aacactttga
     8341 tcgtcagctg ccgctcgatc ggcggcgcag catttcttcg ctgaacgtca gcaacgctaa
     8401 ggtcctacac ctaccgcttt ttgctgcact ggacgcgtcg agaacgta
//
LOCUS       DASSGQ010000156         5252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_467114,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000156 DASSGQ010000000
VERSION     DASSGQ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467114"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..810
                     /gene="trmD"
                     /locus_tag="VFI55_07015"
     CDS             73..810
                     /gene="trmD"
                     /locus_tag="VFI55_07015"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET7073817.1"
                     /translation="MRIDVVTIFPAYLEPLRQSLPGKAIASGLVDLHVHDLRRWTYDV
                     HHAVDDAPYGGGPGMVMKAPVWGEALDETCSSETLLVVPSPAGALFTQATAQRWSAET
                     HLVFACGRYEGIDQRVMEDAARRMRVEEVSIGDYVLPGGESAAVVMIEAVLRLLAGVL
                     GNPASLRDDSHSPALDRLLEGPSYTRPPSWRGLDVPDVLLSGDHGRIAAWRREVSLQR
                     TRERRPDLVAGQGPANTGGRAGPSEHR"
     gene            complement(818..1774)
                     /locus_tag="VFI55_07020"
     CDS             complement(818..1774)
                     /locus_tag="VFI55_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015303628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073818.1"
                     /translation="MRARPLAVLAAMAALTMALAGCDEALVEEQVQAKEPGRPAPTEP
                     ARLSSPQPGSVQLLLGTSTMPGTPQVLSGSGFAGLQTRVQQATDEAAASGAKLSVAVL
                     DRKTRELVSSGNTQIIGTASVAKLFIADDLLLQESQGKTTLSPDDRQALDVMLQSSDD
                     GAAEKFWGQDGGSAIITQVASRYGLSSTTPPSDGRWWNTMSSLTDLIHYYDMLLDGSG
                     GLPQQRATVIVNDLSRSTPSGVDGYPQRFGIPDGLFAEPVAVKQGWMCCIGADWMHLS
                     TGVIGADRRYIMVVQSLQPSDDATARQTITQAVKRIFPSGRI"
     gene            2126..2467
                     /gene="rplS"
                     /locus_tag="VFI55_07025"
     CDS             2126..2467
                     /gene="rplS"
                     /locus_tag="VFI55_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET7073819.1"
                     /translation="MNRLDFVDQASLRDDIPAFSPGDTINVHVKVIEGAKERIQVFKG
                     VVIRRQGGGIRETFTVRKESYGVGVERTFPVHSPTIDHIEVVTRGDVRRAKLYYLREL
                     RGKKAKIKEKR"
     gene            2568..3452
                     /gene="lepB"
                     /locus_tag="VFI55_07030"
     CDS             2568..3452
                     /gene="lepB"
                     /locus_tag="VFI55_07030"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET7073820.1"
                     /translation="MTDTPDSLKDSSEPQPDAGQSEPKVSTRHPDAPAHEPPPPGPPE
                     AAEPERKKKSTLREIVILAVIAVVLYYVMLTFVARPYLIPSESMEPTLHGCTGCVGDR
                     IMVDKLSDRFGAPHPGDVIVFKGPSSWNAGYKSIRSNNSALRWVQNALSFIGFVPPDE
                     NDLVKRVIAVGGQTVQCRADTGLTVNGKPLKEPYLDPATMMADPSVYPCLGSEFGPVT
                     VPAERLWVMGDNRTHSADSRAHCTSIPAEALKGVLCTGDPMSGTVPVSNVIGKARFIV
                     WPPSRWGGVESVNPQQSQ"
     gene            3455..4208
                     /locus_tag="VFI55_07035"
                     /pseudo
     CDS             3455..4208
                     /locus_tag="VFI55_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217418.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
     gene            4281..4586
                     /locus_tag="VFI55_07040"
     CDS             4281..4586
                     /locus_tag="VFI55_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HET7073821.1"
                     /translation="MSAEDLEKYETEMELSLYREYKDIVGQFSYVVETERRFYLANSV
                     EIVPRNADGEVYFELRLSDAWVWDMYRPARFVKQVRVVTFKDVNIEEVEKPELRLPE"
     gene            complement(4609..>5252)
                     /locus_tag="VFI55_07045"
     CDS             complement(4609..>5252)
                     /locus_tag="VFI55_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HET7073822.1"
                     /translation="AICIAATLTVRGCDHASLLVRDNDRYITVGASDRVAQTIDEMER
                     RAGDGPCIDAIEEETPQIDTDLTTASLWPGLASVLLAETPVRGAMGFRLLVDKRKGAA
                     LNLFSDTPNLFDTESAGRAAVLAAFASVAINAVAKGEDSASLRRGLLSNREIGKAVGM
                     LMLLHDLTEDQAFDLLRRHSQALNIKLAEVARVVIESRGQLPANAENQPPQGS"
BASE COUNT          846 a         1709 c         1830 g          867 t
ORIGIN      
        1 gctggacgac cgcatgatcg agatcgatcc gcccgagggc ctgctggacc tggactgaag
       61 gcgcgtaccg agatgcgaat cgacgttgtg acgattttcc cggcgtacct ggaaccgctg
      121 cggcaatcgt tgccgggcaa ggccatcgcg tcgggcctgg tcgatctgca cgtgcacgac
      181 ctgcgccggt ggacctacga cgtgcaccat gcggtggacg acgcacccta cggcggcggg
      241 cccgggatgg tgatgaaggc accggtgtgg ggagaggcgc tggacgaaac ctgttccagc
      301 gaaacgcttt tagtggtacc gagccccgcc ggagcgttgt tcacgcaggc gaccgctcag
      361 cgctggagcg cagagaccca cctggtgttc gcgtgcggcc gctacgaggg catcgaccag
      421 cgggtcatgg aggatgcggc gcggcggatg cgcgtcgaag aggtctccat cggcgactac
      481 gtgttaccgg gcggagagtc ggcggccgtg gtcatgatcg aagccgtact acggctgctg
      541 gccggagtcc tgggcaaccc cgcctcactc cgcgacgatt cgcactcgcc ggccctggac
      601 cggctgttgg aggggccgag ctatacccgg ccgcccagct ggcgtgggct ggacgtcccc
      661 gacgtcttgt tgtccggtga tcatgggcgg atcgccgcct ggcgccgcga ggtctcgctg
      721 cagcggaccc gcgaacgccg gcccgacctg gtggccgggc agggcccggc gaacaccggt
      781 ggccgggcag ggcccagcga acaccgatga aggagggtca gatccggccg ctggggaaga
      841 tccgcttgac ggcctgggtg atcgtctggc gcgcggtggc gtcgtcggag ggctgcagcg
      901 actggaccac catgatgtag cggcggtccg cgccgatcac gccggtcgac aggtgcatcc
      961 agtcggcgcc gatgcagcac atccagccct gcttgaccgc caccggttcg gcgaacaggc
     1021 cgtcgggaat gccgaaccgc tgcgggtatc cgtcgacgcc gctcggggtg gaccgggaca
     1081 gatcgttgac gatgaccgtg gcccgctgct ggggcaggcc gcccgacccg tcgagcagca
     1141 tgtcgtagta gtggatcagg tcggtcaggg agctcatggt gttccaccag cggccgtcgc
     1201 tgggcggggt ggtggaggac agtccgtacc gggacgcgac ctgggtgatg atggcgctgc
     1261 cgccgtcctg gccccagaac ttctcggccg cgccgtcgtc ggacgactgc aacatgacgt
     1321 ccagtgcctg gcggtcgtcc ggggacaggg tggtcttgcc ctgcgactcc tgcagcagca
     1381 gatcgtcggc gatgaacagt ttggcgaccg aggcggtgcc gatgatctgg gtgttgccgc
     1441 tggagaccag ctcgcgtgtc ttgcgatcga gtacggccac cgacagcttg gccccgctgg
     1501 cggccgcttc gtcggttgcc tgctggacac gtgtctgtag gccggcgaag ccgctgccgg
     1561 acagtacctg cggtgtcccc ggcatcgtgc tggtgcccag cagcagttga accgatcccg
     1621 gttgcggtga cgacagccgg gcgggctcgg tcggcgccgg cctgccgggt tccttcgcct
     1681 ggacctgctc ctcgaccaat gcctcgtcgc accccgccag cgccatcgtc agcgccgcca
     1741 tcgcggcgag cacggcgagc gggcgggcgc gcattcctcg atctcctccg gcgtaaacgt
     1801 gcacatgatc gggccgcgtc gccatctgcc ccgggcgtca cgctgaggtt caggttcgac
     1861 aagcgcgttt aggccccagt catatgtacc atccaccggc gccttcgtcc cgttacgaac
     1921 cgcgacgttt acatgtgatt ttcaatgcac ccggggtgtc tggcacaatt gttcagttgt
     1981 ctcgaacccg tcagcgggct gggtcgcttc ccgcctgctg cgaggtacgc actgtaagcc
     2041 cgaaacccat cggcttggtg accgccgcac gcccgcgtgt gggcggctgc cgcaagccgc
     2101 gaccgcaagg aagtgttttc tcacgatgaa caggctggac ttcgtcgacc aggcgtcgct
     2161 gcgcgacgac atcccggcct tcagcccggg cgacaccatc aacgtgcacg tcaaggtcat
     2221 cgagggcgcc aaggagcgta tccaggtgtt caagggcgtg gttatccgcc gccagggcgg
     2281 aggcatccgc gagacgttca ccgtgcgcaa ggaaagctac ggcgtcggcg tcgagcggac
     2341 gttcccggtg cactcgccga ccatcgacca catcgaggtc gtgacacgtg gcgacgtgcg
     2401 ccgcgccaag ctgtactacc tgcgtgaact tcgcggcaag aaggccaaga tcaaggaaaa
     2461 gcgctgagaa gcgctgaccg cgcccccctc ggcggccgtt gcgcatcgcc ggatcgtttt
     2521 cggggcggtc tacccgagcc gcggaccgcg ctgactacgc tgatctcgtg accgacaccc
     2581 cagactcgct gaaggactca tctgagcccc agcccgacgc cggtcagtca gaacccaagg
     2641 tctctacccg ccacccagac gcgcccgcgc acgaaccgcc gccccccggg ccgcccgaag
     2701 cggccgagcc ggagcgcaag aagaagtcca cgctgcgcga gatcgtgatc ctggcggtga
     2761 tcgcggtggt cctctactac gtcatgctga cgttcgtcgc ccgcccgtac ctgatcccgt
     2821 ccgaatccat ggagccgacg ctgcacggat gcaccggttg cgtcggcgac cgcatcatgg
     2881 tggacaagct cagcgaccgc ttcggcgcac cgcatcccgg cgacgtgatc gtcttcaagg
     2941 ggccgtcgtc gtggaacgcc ggatacaagt cgatccggtc gaacaacagc gcgctgcgct
     3001 gggtgcaaaa cgcgctgtcg ttcatcggtt tcgttcctcc cgacgaaaac gacctggtca
     3061 agcgcgttat cgcggtcggt gggcagacgg tgcaatgccg ggccgacacc gggttgaccg
     3121 tcaacggcaa gccactcaag gagccctacc tggatcccgc caccatgatg gcggacccgt
     3181 cggtataccc gtgcctgggc agcgagttcg ggccggtcac cgtgccggcg gagcggctgt
     3241 gggtgatggg cgacaaccgc acgcactcgg ccgactcgcg cgcccactgc accagcatcc
     3301 cggccgaagc gctcaagggc gtgctgtgta ccggcgatcc catgtcgggg accgtgccgg
     3361 tgtccaacgt catcggcaag gccaggttca tcgtatggcc gccctcgcgg tggggtggcg
     3421 tggaatcggt gaacccccag cagagccagt agccgtggcc accgcgtggc cgccgcggac
     3481 ggtgatccgc aagtccgggg gtttgcggac cctggagtcc gcgctgtacc gcaacggtct
     3541 gggcccggtc gccggggtgg acgaggtggg ccggggcgcc tgcgcgggcc cgctggtcgt
     3601 ggctgcctgt gtgctcgggt cgggaagact ggaaagcctt gcggcacttg atgattccaa
     3661 gaagctgagc gaaaaggcgc gggagcagct gttcccggtg atccgccgct acgccctggc
     3721 ctaccacgtg gtctacatcc cgtcggacga ggtcgaccgg cgcggggtac acgtggccaa
     3781 catcgagggc atgcgccgcg cggtggccgg cctgtccgtg cgtccgggtt atgtgctcag
     3841 cgacggattt cgcgtgcccg gcctgcccat gccgtcactg cccgtgatcg gtggcgacgc
     3901 cgtggccgcg tgcatcgccg cggccagtgt gctggccaaa gtcagccggg accgcctgat
     3961 ggtcgcgatg gaccgcgacc accctggcta cggtttcgcc gaacacaagg gctacagcac
     4021 gcccgcgcac tccatggcac tgacccggct gggcccctgc gctgagcacc gctattcctt
     4081 catcaacgtc cggcgcgtgg ctaccgggtc gggtgcgcag gtggtggccg aatgcgcacc
     4141 ggaccctccg gccgagcggg acggataccg gtgagggaag atgggaacac aattttcacc
     4201 cggcgtagac gcgggggccg agcttggagc tccggcaggt agaaggcagg gagaaggacg
     4261 tctgagcaga tgagtggcag atgagcgcag aagatctcga aaagtacgaa accgagatgg
     4321 agctctcgct gtaccgcgaa tacaaggaca tcgtcggcca gttcagctac gtcgtggaaa
     4381 ccgagcgtcg cttctatctg gcaaacagcg tggagatcgt gccacgcaac gccgacggtg
     4441 aggtgtactt cgagctgcgg ctgtccgacg cctgggtgtg ggacatgtac cggccggctc
     4501 ggttcgtcaa gcaggtgcgg gtggtcacct tcaaggacgt caacatcgaa gaggtcgaaa
     4561 aacccgagct gcggctgccg gaatagctgc tggctgccgg aatggctgct agctgccctg
     4621 cggcggctga ttctcggcgt tggccggcag ttgtccgcga ctttcgatga cgacgcgggc
     4681 cacctcggcc agcttgatgt tcagggcctg ggaatggcgc cgcagcaggt cgaacgcctg
     4741 gtcttcggtc agatcgtgca gcagcatcag catgccgacc gccttgccga tttcgcgatt
     4801 gctgagcagc ccgcggcgca ggctggcgga gtcctcgccc ttggcgaccg cgttgatggc
     4861 cacgctggcg aacgccgcca ataccgccgc ccgcccggcc gactcggtgt cgaacaggtt
     4921 gggggtgtcg ctgaacaggt tgagtgctgc ccctttgcgt ttgtcgacga gcagccgaaa
     4981 acccatcgcc ccgcgcaccg gggtctcggc gagcaggacg gacgcgagcc cgggccacag
     5041 cgatgccgtg gtgaggtccg tgtcgatctg gggggtttct tcctcgatcg cgtcgatgca
     5101 cgggccgtca cctgcacgcc gttccatctc gtcgatcgtc tgtgcgaccc gatcgctggc
     5161 gccgaccgtg atgtagcggt cgttgtcgcg caccaacagg ctggcgtggt cgcagccgcg
     5221 gacggtcagg gtggcggcga tgcagattgc gg
//
LOCUS       DASSGQ010000157         5813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1166164,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000157 DASSGQ010000000
VERSION     DASSGQ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166164"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VFI55_07050"
     CDS             complement(<1..773)
                     /locus_tag="VFI55_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HET7073823.1"
                     /translation="MGRVAGKVAVISGAARGQGRSHARLLAAEGADIIAVDLCADIET
                     NEYPLARPEDLDETARLVEKEGRGAFAAIADVRDRVALAAAIDRGVAEFGHLDIVVAN
                     AGICPLTAGLPPQAFADAVDVDLVGVLNLVHASLKHLQAGASIIVIGSNAAFMSSMNT
                     TGIDGGPGGAGYAFAKLAAAHYVNDFALALAKFSIRMNAVHPTNVNTDMLHSAPMYRA
                     FRPDLKEPTREDAEPVFPLVQAMPIPYVEPEDISQAVLFL"
     gene            1068..2321
                     /locus_tag="VFI55_07055"
     CDS             1068..2321
                     /locus_tag="VFI55_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005184573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HET7073824.1"
                     /translation="MITQGVTVSGLHDRVLRGVRVVELASWTYVPSAGAALSDWGADV
                     IKVEGVASGDPGRALVIGGFTREAARPDADFILELGNRGKRSIAVDIKSEIGRDLLGK
                     LLASADVFLTNWLPGALERARLTVADIRAFNPKIIIARGTGLGVRGPDRDRGGFDAAT
                     YLARGGVAYTLTPFGTEPPAVQGPAFGDLQSGATLAGGVCAALFHRERTGEPTIVDSS
                     LLAQAMWAIAPSISAADFFDIDGIPGAPPGLAINPLVNRYKTKDGRWIQLVFLQPDKF
                     WAGFCERMGLAELATDQRFVPSANLIANAAAATEIVATAFASHDLAHWQKVLEDEPGV
                     WGALATPRETLNDPQVEPNGYVVTNVDDHGEKYRIVAAPVQFNETPPTPARAPEHGQH
                     TEEILLELGVDWNDIARAKDVKAIL"
     gene            complement(2607..2840)
                     /locus_tag="VFI55_07060"
     CDS             complement(2607..2840)
                     /locus_tag="VFI55_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073825.1"
                     /translation="MAIENWCDAPQIHPSEIRVGDIIGTVRSTQLRLTVKLISGPQTT
                     PRQWTFFSRDDTGRRYTNTFAEDDFVRRYARAS"
     gene            3228..3653
                     /locus_tag="VFI55_07065"
                     /pseudo
     CDS             3228..3653
                     /locus_tag="VFI55_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724710.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5994 family protein"
     gene            4310..>5813
                     /locus_tag="VFI55_07070"
     CDS             4310..>5813
                     /locus_tag="VFI55_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014588419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HET7073826.1"
                     /translation="MSVLIAAPELVAAAATDLANVGSTITAANAAAAFPTTSLVAAGA
                     DEVSASIAALLGAHAHAYQQLSVQAATFHNQFVQLLNAGANTYQAAEAANVSPLQTAQ
                     QDALNLANAPSQSLTGRPLIGNGANGAPGTGQNGGNGGWLFGNGGNGGSGGTGQGGGN
                     GGSAGLWGSGGNGAAGGNSPVTGGSGPNGGAGGNGGLLWGNGGAGGNGGTGGDGAAIT
                     PTPVNPPFSVPAGAGGAGGNGGKAGLFYGFGGPGGTGGAGGIAPAIGPGGLLGASGDG
                     GPGGAGGAGALALGYGGAGGAGGLGGTSLAGVQTIGGAGGVGGAGGQSGLLFGNSGAG
                     GIGGAGGNGLDTGSFSGQGGTGGAGGAVGLFGNGGAGGNGGVGGQGVSDTSGGVGGLG
                     GNGGPGGLLYGSGGAGGNGGFGGAGSGTGFGGSGGFAGSGGNARLIGSGGVGGDGGGI
                     GTGTPATLVVGGQGGDGGRAGVLFGIGGDGGDGGATSAGGTLFAGGGDGGN"
BASE COUNT          994 a         1853 c         1998 g          968 t
ORIGIN      
        1 agaaataaca cggcctggct gatgtcctcg ggttcgacgt aggggatggg catcgcctgg
       61 acaagcggga agaccggttc ggcgtcctcg cgggtcggtt ccttgaggtc gggccggaat
      121 gcacggtaca tcggcgcgct gtgcaacatg tcggtgttga cgtttgtggg atgcaccgca
      181 ttcatccgga tggagaactt ggccagcgcc aaggcaaagt cgttgacgta gtgggcggca
      241 gcgagtttgg cgaaggcgta gccggccccg cccggaccgc cgtcgatacc ggtggtgttc
      301 atcgacgaca tgaatgccgc gttggatccg atcacgatga tcgacgcgcc ggcttgcaga
      361 tgtttgaggc tcgcgtgcac caggttgagc acgccgacca ggtccacgtc gacggcgtcg
      421 gcgaacgcct ggggcggtag gccagcggtg agcgggcaga taccggcatt ggcaaccacg
      481 atgtcgagat ggccgaactc ggccacaccc cgatcgatgg cggcggccaa ggctacgcgg
      541 tcgcgtacgt cagcgattgc cgcgaacgca ccccgcccct ctttttcgac cagccgcgcg
      601 gtctcgtcga gatcctccgg ccgggccagc gggtactcat tggtttcgat atctgcgcag
      661 agatccaccg cgatgatgtc ggcgccttcc gcggcgagta gccgtgcatg cgaacgtccc
      721 tgaccgcgcg cggccccgct gatgacggcc accttgcccg ctactctgcc catcgcgcgt
      781 ccttcgtccc agtgccgact gcgacgaccc gcattgggag ccgttgggag ccaacgccgt
      841 tcgccaagag cacgggcgcg gcgggtgtca tcggtggcag gccgacccgg gattgtgtga
      901 ccggaagtgt ggtcggtgcc cgcagacctg cgccgtgaca cacgtctgcc gcatcgcgga
      961 tcgggatcgg acaaacggtc ggctccatcg catctcttcc acgcgcacag ctgtagaagg
     1021 gctatcgata tcccttacag tagctagaat atctaaaata gcaagaaatg atcacccagg
     1081 gagttacggt gtccggcttg cacgatcgcg tccttcgagg tgtccgagtt gtcgagctgg
     1141 cttcctggac ctacgtgccg tcggccggtg ccgcgctatc cgactggggc gccgacgtca
     1201 tcaaggtgga aggtgtcgcc tcgggtgatc ccgggcgcgc gctcgtcatc ggcggcttca
     1261 cccgggaagc cgcacgcccc gatgccgact tcatcctcga gttgggcaac cgcggcaagc
     1321 gcagcatcgc ggtcgacatc aaatccgaaa tcggccgaga cttgttgggc aagctgttgg
     1381 ccagtgccga tgtcttcctg accaattggc tgcccggcgc actcgagcgg gcgcgattga
     1441 cagtcgccga catccgcgcg ttcaacccga agatcatcat cgcgcggggt accggactgg
     1501 gggtccgggg cccggatcgg gatcgtggcg ggttcgacgc cgcgacgtat ttggcgcgtg
     1561 gcggggtcgc gtacacgctc acgccgttcg gcaccgaacc gcccgccgtc cagggcccgg
     1621 cattcggcga cctgcagagt ggggcgacgc tggcgggtgg cgtgtgtgcc gcgttgtttc
     1681 atcgggaacg gaccggtgaa ccgacgatcg tcgactcctc gctgctggcc caggcgatgt
     1741 gggcaatcgc gccgtcgatt tcagcggccg atttcttcga tatcgacggc attccgggtg
     1801 caccgcccgg gttggccatc aatccacttg tcaaccggta caagaccaaa gacggcaggt
     1861 ggattcagct ggtgttcttg cagcccgaca aattctgggc cggtttctgt gaacggatgg
     1921 gtctggcgga gttggccacc gaccaacgat ttgtgccgtc ggccaacctg atcgccaacg
     1981 ccgcagcggc taccgagatc gtggcgaccg ccttcgccag ccatgacctg gcgcactggc
     2041 agaaggtgct cgaagacgaa cccggcgtgt ggggcgcttt ggcgacgccg cgcgagaccc
     2101 tcaacgaccc gcaagtagaa cccaatgggt acgtggtgac aaacgtcgac gaccatgggg
     2161 agaaataccg gatcgtcgcc gcgccggttc aattcaatga aacaccgccg acccccgcgc
     2221 gggcaccgga acatgggcag cacaccgaag agattctgct cgaactcggc gtcgactgga
     2281 acgatatcgc cagggcgaag gacgtcaaag ccatcctctg aggcacgccg gcccgataca
     2341 ccccgatggt cggacggaac aacgcgaggg tcgacaccac acagcccaaa agccaacccg
     2401 tacaagagga ttaccgacgg ccttccgcat caacgccgga tgggtgaggt tgatggtggc
     2461 gaacttctgc ttttgcgcca acctgaatgg ggctgagcaa cgtggaaggt ggcccgggtt
     2521 gcggcgagcg caatactcaa gtctcgcagg gtgatagaga cggtaggtcc aggttggccg
     2581 agttctagga ctgcgctagg accttgtcat gatgctctgg cgtatctgcg aacaaagtcg
     2641 tcctctgcga acgtgttcgt gtaccgccgg ccggtgtcgt cacggctgaa aaatgtccat
     2701 tgccgcggag ttgtctgcgg accactgatc agcttgacgg tgaggcgcaa ttgggtggac
     2761 cgcaccgtgc ctatgatgtc gccaacccgg atctccgatg gatggatctg gggtgcgtcg
     2821 caccagttct ctattgccat gcacccgtcc tcaacttgtt gccatcacac tacgcgcaat
     2881 cagcgtctat cgctgacacg ggcagtcgcc gagcgccgcg cttacgcgct gtctctggtt
     2941 acctgatcgc ccagtccggc cgctggtggc gtagcgtcaa gtgcgagccg tttcgttgtc
     3001 agcggtggtc cactgcgcaa acggcgccga tgacacccac gggcaatgtg attcctccgt
     3061 ccgtcccgcg catgtgggcc gacgatgctg accgtgaagg aaccgcccat gggttgccaa
     3121 gccccggcaa tcgcccgaca gccatacatt ccgagtccgg cttttgcgca caagtgcggc
     3181 accgtgactg tcgggtgcgc gctctgccga cttaggcaac tccgatgatt catccgatcg
     3241 cgggccgacg ccaagccaat tccatccggc tgtcgctggc tcgcgagctc ggccgcgaca
     3301 tcgacggcgc atggtggcca cgtgcagatc gcatcaccaa tgaattgccg aatctcgtcg
     3361 cggtcctcac tcccttgctc ggagacatca cctctatcaa cgtcaactgg tcaccactgc
     3421 aacgaccacc cgatctcaac tggaccggat ggcaacacaa acgccagcac gtaatgaccg
     3481 tcaccggcac gcacgctcgc gcgaacctgc tggtcatctc ttacgcgacc cacagcgcgc
     3541 tggccctact cgtgctgcgc tgcgccgcta accttcacat tgaggctgct gaccgcgaca
     3601 aacccgcatt tccgatcgcc gggtcgatcc tgctggccgc ccaacgacaa cgcgcggccg
     3661 gcctgtcgct ggccccaatc gacgccgacg accgcccggc ggtccccgct gtgcgcttgg
     3721 cgcgcagtcg ttgcggggcg cctcagactt ctccacagtc ggaagtccga tgaatcaagc
     3781 tgtctcgcaa tagattacag aaccaacgac tcgagtggcg actgtcttaa gccgtcggtc
     3841 atttcttcga cttccgacca tatgcagctg agtttcatcc tgtccagctg atctcgacag
     3901 gacgcaaccc ggcggcgaat ggtggcaata tcgctgtgcg aacgatgatt tcgcacacgt
     3961 agttgactac gccaccgagt ggaagccggt cgaccgtggc gtggctgatg gccttcgcac
     4021 ccggttcgcc gcacctagtg cagctgcggt cgagctcgcg gcgagaatac gcaccgcaga
     4081 tgcggcgcca tcctgctgca cggcgcaccg gccaccaccg acgattctca gggctacacc
     4141 gcgttctgcg aattctctga caccacgccg gcatagccgt cgcggacccg cgggccgaca
     4201 gaggtgaaac gtttcggggc cgcaccccga attggacaat aggttcagcc cggatgcgga
     4261 gcggtgaaca ggcgcccgcc cgatttcgac aagatggtgg aaggtccgta tgtcggttct
     4321 gatcgccgca ccggaactgg tggcagcggc agctacggat ttggcgaacg tgggatcgac
     4381 gatcacggcg gctaacgccg ccgcggcatt tccgacgacg tcgctggtcg ctgcgggcgc
     4441 cgacgaggta tcggcgagta tcgccgcgtt gttgggcgcg catgcccacg cataccagca
     4501 gctgagcgtg caggccgcga cgtttcacaa ccaattcgtg cagctactga atgcgggcgc
     4561 caacacgtac caggccgccg aggcggcgaa cgtgtcgccg ttgcagaccg cccaacagga
     4621 cgcgctcaac ctggccaacg cacccagcca gtcgctcaca gggcgcccgc tgatcggtaa
     4681 cggcgccaac ggcgccccgg gcaccggaca aaacggcggc aacggcgggt ggttgttcgg
     4741 caacggtggc aacggcgggt ccggcgggac cggccagggc ggaggaaacg ggggaagcgc
     4801 cggattgtgg ggcagcggcg gcaacggcgc ggcaggcggc aacagccctg tgaccggcgg
     4861 atccggcccc aacggcggcg ccggcggcaa cggcgggttg ctatggggca acggtggcgc
     4921 cggcggaaat ggcggaaccg gcggcgacgg cgctgccatc accccaacgc cggtgaatcc
     4981 gccgttcagc gttcctgcgg gcgccggcgg cgcgggcggg aacggcggca aggccgggct
     5041 gttctacggc ttcggtggtc caggagggac cggcggggcc ggtggcatag cgcctgcgat
     5101 cggcccgggc gggctgctcg gcgcaagtgg cgacggcggt ccgggcggtg cgggcggcgc
     5161 cggcgcgctt gcgctcggct acggcggtgc gggcggtgcg ggcggcctcg gcggcaccag
     5221 tctcgccggg gtccagacca taggcggcgc gggtggcgtg ggtggggccg gcggccagag
     5281 cggcctactg tttggcaact ccggagccgg cgggatcggc ggtgccggcg gcaacggcct
     5341 tgacaccggc agtttcagcg gtcagggcgg caccggtggc gccggcgggg ccgtcgggct
     5401 gttcggcaac ggcggcgccg gcgggaacgg cggggtcggc gggcaaggcg tgagcgatac
     5461 ctccggaggc gttggcggcc tcggcggcaa cggcgggccg ggtggtctgc tctacggaag
     5521 cggcggcgcg ggcggcaacg gcggattcgg cggagccggg tccggtaccg gctttggcgg
     5581 ctccggtggt ttcgccggtt ccggcggtaa cgctcggctc atcggcagtg gcggcgtcgg
     5641 cggcgacggt ggcggtatcg gcaccggcac gccagcgacc ttggtagtgg gcggtcaagg
     5701 cggcgacggc ggccgagcgg gagtgctgtt cggcattggt ggcgacggcg gcgacggcgg
     5761 cgccaccagt gccggcggga cgctgtttgc cggtgggggt gacggcggca acg
//
LOCUS       DASSGQ010000158        15661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_346417,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000158 DASSGQ010000000
VERSION     DASSGQ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15661
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346417"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VFI55_07075"
     CDS             <1..557
                     /locus_tag="VFI55_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015289573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid droplet-associated protein"
                     /protein_id="HET7073827.1"
                     /translation="PKTILMYPMTLVSQAAHLVMRFQQSLAELVTKGDSTLESIFPPK
                     DEKPEWVTFDEDLPDAGGNPAGIAGALPEGIDSDRRAEGRFALYSVSDAPPETSEAVA
                     AEPSTTPKAPRPVKKSAAKAVPEPAVAVELDYPTLTLAQLRARLQSLDVDELEALLAY
                     EQATKARAPFQTLLANRITRANSK"
     gene            554..1804
                     /gene="xseA"
                     /locus_tag="VFI55_07080"
     CDS             554..1804
                     /gene="xseA"
                     /locus_tag="VFI55_07080"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET7073828.1"
                     /translation="MTRAADSQPNSAENPFPVRAVAIRVAGWIDKLGTVWVEGQLAQI
                     TMRPDAKTVFMVLRDPAADMSLTATCPRDVVLNAPVKLAEGTQVVVCGKPSFYTGRGT
                     FSLRLSEIRAVGVGELLARIDRLRRLLDAEGLFDPRLKRPIPFLPNMIGLITGRASAA
                     ERDVTTVAAARWPAVRFAVRNTAVQGPNAVPQIVEALSELDRDREVDVIVLARGGGSV
                     EDLLPFSDESLCRAIAACRTPVISAVGHEPDNPLCDLVADLRAATPTDAAKKVVPDTA
                     AEQRLIDDARRRSAQALRNWVSREQRALAQLRSRPVLAEPLRALTARADEIHRARSAV
                     RRDIGRLVAAETERVGHLSARLATLGPAATLARGYAVVQTVAAAGPATVLRSVDEAPT
                     GTRLRVRVTDGAITAVSEGPTDGH"
     gene            1794..2057
                     /locus_tag="VFI55_07085"
     CDS             1794..2057
                     /locus_tag="VFI55_07085"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET7073829.1"
                     /translation="MVTRRNSAEGGDETAGTTTPISQLGYEACRDELIEVVRLLEQGG
                     LDLDASLKLWERGEQLAKRCEEHLAGARKRVADALASGQAEED"
     gene            2143..3267
                     /locus_tag="VFI55_07090"
     CDS             2143..3267
                     /locus_tag="VFI55_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003913657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7073830.1"
                     /translation="MADASLTTELGRVLVTGGSGFVGANLVTTLLNRGYQVRSFDRVP
                     SPLPRHDALEVLQGDITDAGVCAAAVHGIDTVFHTAAIIELMGGAAVTDAYRRRSFAV
                     NVGGTENLVHAGQAAGVRRFVYTSSNSVVMGGQNIAGGDETLPYTDRFNDLYTETKVV
                     AERFVLSQNASPNAGGGMLTCAIRPSGIWGRGDRTMFRKLFESVIAGHVKVLIGRKSA
                     RLDNSYVHNLIHGFILAAEHLMPGGTAAGQAYFINDAEPINMFEFARPVMEACGENWP
                     RIRVNGPMVHAAMTGWQRMHFRFGIPAPLLEPLAVERLYLDNYFSIDKARRDLGYEPV
                     FTTAKAMSECLPYYVELFRQMKSHALAAKTIPKTTVSGSR"
     gene            3361..4545
                     /locus_tag="VFI55_07095"
     CDS             3361..4545
                     /locus_tag="VFI55_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7073831.1"
                     /translation="MKTEFTLNQKRALAVLTLIALLFAGYFLRDFFVLIVVAAVGAYL
                     FTPVFNRFNRRLGTGLSATCTLLTALAIVAVPIALLIVLAIVQISRMIDSVAGWVKTT
                     DLSGLGDKALHLVNEFAARIPFVHITVTAEMLRKAMVTGAQNVGQWLLHFLQGAVGGI
                     AGAITAAIIFLYVFLALLVNREKVRTLIGQLNPLGDEVTDLYLTKAGAMVRGTVNGQF
                     VIALCQGVSGAASIYIGGFHHGFFIFAVLLTALSIIPLGSGVVTIPFGIGMILYGNVV
                     GGAFVVLFHLVGVTNIDNFLRPILVPKDARLNSALMLLSVFAGIAMFGPWGIVIGPVL
                     MILIVTTIDVYLAVYKGVELQAPDEEPVHRNWFPRRKANESAREAGEAGESKESEKSE
                     AD"
     gene            complement(4559..6127)
                     /locus_tag="VFI55_07100"
     CDS             complement(4559..6127)
                     /locus_tag="VFI55_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HET7073832.1"
                     /translation="MAIDECVVDTTYGPVRGSDAGLAGGRVKTWKGIRYAAPPVGDLR
                     FRVPEPPRPWTQVADATAFGPACPQPTFPNMPLDLGAPQGEDCLRLNIWAASDTQPGD
                     RKPVMVWLHGGAYILGSGSQALYDGRRLAAGGDSDAGVVVVTVNYRLGALGFLDLSSF
                     NTARRRFDSNIGLHDVLEALRWVRDNIAAFGGDPHKVTLFGESAGAGIVTTLLATPAA
                     EGLFAGAIAQSSPATGVYDRDRARRVAVGILGKLGIEPSDVDRVLDVPTAAMLAASQQ
                     AFDEVPARNPGTLAFVPIVDGELLHDYPVKLVQEGCSHPVPLIIGTNKREAALFRLMR
                     SHLMPITPHAIKAMFAQIAAEQPDLQLPTEQQIGSAYSRMRGKARPLGIATDLGFRMP
                     SVWLAEGHSRVAPVYLYRFDYATPFWKLLLTGPAHATELPYVWGNLGAPRDPTLKLGG
                     AKTAKAVSKRVRTRWVNFAAQGKPAGPAGEPDWAPYRGVDRASLIIDKRDRVVRDVDG
                     HIRAAWGSEVVSFR"
     gene            complement(6143..6940)
                     /locus_tag="VFI55_07105"
     CDS             complement(6143..6940)
                     /locus_tag="VFI55_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET7073833.1"
                     /translation="MTAPEADLSGWSAAPFSDGGYTHDVYRKGAGPGVLLIPELPGIT
                     PGVLGLGDHLVDNGFTVAIPSLFGEPAKPVTNRYVTAAIARACVAREFAAFATNRQRP
                     VSLFLRGLARDLNASTPGKGVGVIGQCFTGGFALAAAVDDSVLAPVLSQPSAPFPLGD
                     ARRRDPGLSESELATVADRCANDGLCALGLRFSEDWKAPRERFATLKARLGDAFEVIE
                     IDSGPGNAGGFDKSAHSVLTEEVREVDGQPAYEARKRVVEFLTARLS"
     gene            complement(6968..7654)
                     /locus_tag="VFI55_07110"
     CDS             complement(6968..7654)
                     /locus_tag="VFI55_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4245 domain-containing protein"
                     /protein_id="HET7073834.1"
                     /translation="MTEEARPNAQVGEPAPAGWPVPKPAKPRLLQDGRDMFWSLVPLV
                     VGCILLAGLVGMCSFQPGRTNNSVIPSYDATAALRADAQTLGFPVRLPQLPAGWRPNS
                     GGRGGIENGRTDPSTGRRLNAATSIVGFISPTGMYLSLTQSNADEDKLVGSIHPAAYP
                     IGTVDVDGTSWVVYRGAGQAGADAEPVWTTRLTGPAGTTQIAITGAGSTDQFRTLAAA
                     TQSQPPLASR"
     gene            7735..8835
                     /gene="glpX"
                     /locus_tag="VFI55_07115"
     CDS             7735..8835
                     /gene="glpX"
                     /locus_tag="VFI55_07115"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215615.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HET7073835.1"
                     /translation="MTAEGDRPLGTGSSPTATADRNPAQVRPRGEAPDRNLALELVRV
                     TEAGAMAAGRWVGRGDKEGGDGAAVDAMRELVNSVSMRGVVVIGEGEKDQAPMLYNGE
                     EVGNGDGPECDFAVDPIDGTTLMSKGMPNAISVLAVADRGAMFDPSAVFYMNKIAVGP
                     EAAHVLDITAPIAENIRAVAKVKALSVRDMTVCILDRPRHAQLIEDVRATGARIRLIT
                     DGDVAGAISACRPESGTDLLAGIGGTPEGIIAAAAIRCMGGAIQAQLAPRDEAERRKA
                     LAAGYDLDQVLTTEDLVSGENVFFCATGVTEGDLLKGVRYYPGGCTTHSIVMRSKSGT
                     VRMIEAYHRLSKLNEYSAIDFTGDSSAAYPLP"
     gene            8866..10284
                     /locus_tag="VFI55_07120"
     CDS             8866..10284
                     /locus_tag="VFI55_07120"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HET7073836.1"
                     /translation="MAADGSQTDTEYRIEHDTMGEVRVPAKALWRAQTQRAVENFPIS
                     GRGLERTQIRALGLLKGACAQVNKDLGLLAPEKADAIIAAAAEIADGRHDDQFPIDVF
                     QTGSGTSSNMNTNEVIASIAAANGVAVHPNDDVNMSQSSNDTFPTATHIAATEAAVRH
                     LIPALRELHDALAAKAHEWHTVVKSGRTHLMDAVPVTLGQEFSGYARQIEAGIERVSA
                     TLPRLGELAIGGTAVGTGLNAPDGFGGKVVETLVAETGLSELRTASNSFEAQAARDGL
                     VEASGALRTIAVSLTKIANDIRWMGSGPLTGLAEIQLPDLQPGSSIMPGKVNPVLPEA
                     VTQVAAQVIGNDAAVAVGGLSGAFELNVYIPMMARNILESFKLLTNVSKLFAQRCIVG
                     LKANVEHLRELAESSPSIVTPLNSAIGYEEAAAVAKQALKERKTIRQTVIDRGLIGDK
                     LSIEELDRRLDVLAMAKVKPED"
     gene            10471..11463
                     /locus_tag="VFI55_07125"
     CDS             10471..11463
                     /locus_tag="VFI55_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073837.1"
                     /translation="MTAPPGGPYNQDPYGANPYGQGPYWGGQPQGGSYPYPPPGSPYA
                     HPHGAADPYAAGPYPPQQYPQPGQQVPPGWPPPGGYPPGPPPKGPRSKAPWVILAAVA
                     VLGVIALVIILVIGLNDRNKPSSANPSGPAGPAPTTTGAPTSQPNNAGQTATGCTPNV
                     SGGDKPVGDTVSAGKLSFAASAAPGWSGFSDDQTPNLIQAVGIGQEVPTASQWMMQAE
                     VAVTNFVASMDVGAQASKLMDCVANGPGYANATPTLGPMKTSSITVDGVNAARVDADV
                     AIADTARNVKGDSLVIIAVNTKPVTIFLGATPIGDAGSANIINQVIASLKVSKK"
     gene            complement(11450..12970)
                     /locus_tag="VFI55_07130"
     CDS             complement(11450..12970)
                     /locus_tag="VFI55_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7073838.1"
                     /translation="MSIRYAIDLALAYLLAVVDAAAILIPLRGHTSVAANADFAQKNT
                     AVVIAFVALGTVGVVIAGVVTLAPTLRWYVAGDEPTAAQREIAMKIPGRQSAILVLAW
                     SVAGAFFALLNRHGGPQLLLPILLGVLLGGPAAAGIGMLLAQRTLRPIMGVATLGCEP
                     RLAVPGVFARMVLLWFLCSAIPIGVIAALVVLRSYGWLIQKSASLDVPVLVVSLAALL
                     LGLPTMILTSRSISDPVGEVVDAMAEVEHGRIGTSVGAYERSQIGRLQTGFNRMVAGL
                     AERDRLRDLFGRHVGADVAQRAIQEGASLSGDVVEAAVLFIDLVGSTQLAESRPPQEV
                     AEVLNDFFRIVVGAVDERHGLINKFAGDAALAVFGAPLRMSEPTKPSSAALMTARALG
                     TQLRRLPVVDFGIGVSAGRVFAGNIGAENRYEYTVIGDAVNEAARLADLAKTADRRIL
                     CSAAAVDRADEAERARWAECYSTVLRGRSEATHVSAPVAPASPQIAWRTTGHVTSC"
     gene            complement(13103..13978)
                     /locus_tag="VFI55_07135"
     CDS             complement(13103..13978)
                     /locus_tag="VFI55_07135"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7073839.1"
                     /translation="MPKRPDSQAWRYWRTVFGVVAAAAVLVIGGLTGHVTRADNPGCS
                     VVKCIALTFDDGPGPYTDRLLQILKDSDAKATFFLIGNKVAANPAGAKRIADAGMEIG
                     SHTWEHPNMTTIPVDDVAAQFSRANDAIAAATGRTPSLWRPAGGLSDDVVRQTAAKFG
                     LGEILWDVIPFDWINDSNTAATRYMLMTYIKPGSVVLFHDTYSSTVDLVYQFIPVLKA
                     NGYRLVTISELLGSRAPGSSYGGRENGPPVNALHDIPPGEIPTLPNTPSPKPMPNFPI
                     TDIPGQNSGGPNNGA"
     gene            complement(14132..15433)
                     /locus_tag="VFI55_07140"
     CDS             complement(14132..15433)
                     /locus_tag="VFI55_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET7073840.1"
                     /translation="MTEIRTYVLDTSVLLSDPWACSRFAEHEVVVPLVVISELEAKRH
                     HHELGWFARQALRLFDDLRLEHSRLDQPIPVGTQGGTLHVELNHTDPAVLPAGFRTDS
                     NDSRILSCAANLAAEGKRVTLVSKDIPLRVKAAAVGLAADEYHAQDVVVSGWSGMQEI
                     ETGAEDIDALFADGEIDLAEARDLPCHTGIRLLGGGSHALGRVTPAKRVQLVRGDREV
                     FGLRGRSAEQRVALDLLLDESVGILSLGGKAGTGKSALALCAGLEAVLERRTHRKVVV
                     FRPLYAVGGQELGYLPGSESEKMGPWAQAVFDTLEGLASPAVLEEVLSRGMLEVLPLT
                     HIRGRSLHDSFVIVDEAQSLERNVLLTVLSRLGAGSRVVLTHDIAQRDNLRVGRHDGV
                     AAVIEKLKGHPLFAHITLLRSERSPIAALVTEMLEEIAGPQ"
BASE COUNT         2675 a         5344 c         5213 g         2429 t
ORIGIN      
        1 tgccgaaaac catcctgatg tacccgatga cgctggtcag tcaggcggcg cacctggtga
       61 tgcgattcca gcagagcctg gctgagctgg tgaccaaggg cgacagcacc ctggagtcca
      121 tcttcccgcc caaggacgag aagccggagt gggtcacctt cgacgaagac ttgcccgacg
      181 ccgggggaaa ccccgcggga atcgccgggg cgctcccgga gggcatcgac agtgatcgcc
      241 gggccgaggg acggttcgcg ctgtactccg tatccgatgc gccgcccgag acctccgagg
      301 ccgtcgccgc cgaaccttcg accacgccca aggcgccccg gccggtgaag aagtcggccg
      361 ccaaggcagt tccggaaccg gcggtggcag tcgaactcga ctatccgacg ctgacgctgg
      421 cccagctgcg ggccaggctg caatcgctgg atgtcgatga actcgaagcc ctgctggcct
      481 acgagcaggc caccaaggcc cgtgcaccgt tccagacgct gctcgccaac aggatcaccc
      541 gcgcgaacag caagtgaccc gcgcggcaga ttcccaaccg aactccgccg agaacccctt
      601 tccggtgcgc gcggtggcga tccgggtcgc gggctggatc gacaagttgg gaaccgtatg
      661 ggtagagggg cagttggccc agatcaccat gcggcccgac gccaagacgg ttttcatggt
      721 actgcgcgac ccggccgccg acatgtcgct gacggcaacg tgtccgcgcg acgtggtcct
      781 caacgcgccg gtgaagctcg ccgaaggcac tcaggtggtg gtgtgcggaa agccctcgtt
      841 ctacacgggg cggggaacgt tttccctgcg cctcagcgag attcgcgctg tcggcgtcgg
      901 ggagctgctg gcacgcatcg accggctgcg ccggctgctg gatgccgaag ggctcttcga
      961 cccgcggctg aagcgcccga tcccgttcct gccgaacatg atcggcctga tcacgggccg
     1021 ggccagcgcc gccgaacgtg acgtgacgac ggtggcggcc gcgcgctggc ccgcggtgcg
     1081 tttcgcggtg cgcaacaccg ccgtgcaggg acccaacgcg gtcccccaga tcgtcgaagc
     1141 actgtccgaa ctcgatcgcg accgggaggt cgacgtgatc gtgctggccc gtggcggcgg
     1201 tagcgtcgag gacctgctgc cgttctccga cgagtcgctg tgccgcgcga tcgcggcctg
     1261 ccgcacaccg gtgatcagcg cggtggggca tgaacccgac aatccgctgt gcgacctggt
     1321 tgccgacctg cgcgccgcca cacccaccga cgcggccaag aaggtggtgc cggacaccgc
     1381 tgccgagcag cggctgatcg atgatgcgcg ccggcgcagc gcacaggcgc tgcgcaattg
     1441 ggtgtcccgc gaacaacgcg cgctggccca gttgcgcagc cgaccggtgc tcgccgagcc
     1501 gttgcgggcc ctgacggcgc gcgccgacga aattcaccgc gcgcgctcag cggttcgccg
     1561 cgacatcggc cggctggtcg ccgccgagac ggagcgcgtc ggtcatctca gcgcgcggct
     1621 ggccacgttg ggcccggcgg ccacgttggc ccgcggctat gccgtcgtgc aaaccgtcgc
     1681 cgccgccgga cccgcgaccg tgctgcggtc ggtggacgag gcccccacgg gcacccggct
     1741 acgggtgcgg gtaaccgacg gcgccataac agcggtgagc gaaggaccaa ccgatggtca
     1801 ctaggagaaa cagtgcggaa ggcggcgacg aaacggccgg tactacgacg cctattagtc
     1861 agctcggcta tgaggcctgc cgcgacgagc tgatcgaagt ggtacggctc ctggaacagg
     1921 gcgggctgga cctcgatgcg tcgctgaagc tgtgggaaag aggcgaacag ctcgcaaaaa
     1981 ggtgcgaaga gcacttagct ggcgcgcgta agcgggtggc cgatgcgctc gcatccggcc
     2041 aggccgagga ggactgagcg caggccgatt acgaagcgga tctttcgaac ctccgctcga
     2101 attggaacac gtttcacctg cctcggctat gctcgcagca ctatggctga tgcatcactg
     2161 acaaccgaac tcggccgggt cctcgtcacc ggcggctcgg ggttcgtcgg cgccaacctg
     2221 gtgacaacct tgctcaaccg cggataccag gtacgttcct tcgaccgcgt cccgtcgccg
     2281 cttccccggc acgacgccct ggaggtgctg cagggcgata tcaccgacgc gggcgtctgc
     2341 gccgcggcgg tacacggtat cgacacggtc ttccacaccg ccgcgatcat cgagctgatg
     2401 ggtggcgcgg cggtgaccga cgcataccgc cggcgcagct tcgcggtcaa cgtcggcggc
     2461 accgagaacc tggtgcacgc ggggcaagcg gccggagtgc ggcggttcgt ttacacctcg
     2521 tccaacagcg tggtgatggg cgggcagaac atcgccggcg gcgacgagac gctgccctac
     2581 accgaccggt tcaacgacct ctacaccgaa accaaggtgg tcgctgagcg attcgtgttg
     2641 tcccagaacg cgtccccgaa cgccgggggc ggcatgctga cgtgcgcgat ccggccgagc
     2701 ggcatctggg gtcgcggcga tcggacgatg tttcgcaagc tgttcgagag tgtgatcgcc
     2761 ggccacgtca aggtgctgat cggacggaaa tcggcccggc tggataactc ctacgtgcac
     2821 aacctgattc atggtttcat cctggctgcc gaacacctga tgcccggtgg aaccgcggcc
     2881 ggccaggcct acttcatcaa cgatgcggag ccgatcaaca tgttcgagtt cgcccgtccg
     2941 gtgatggagg cctgcgggga aaactggccc cgaattcggg tcaacgggcc gatggtccac
     3001 gccgcgatga cgggatggca gcgcatgcac ttccgattcg ggataccggc gccactgctg
     3061 gaaccgctgg cggtcgaaag gctatatctg gacaactact tctcgatcga caaggcgcgg
     3121 cgcgacctcg gctatgagcc agtgttcacc accgcgaagg cgatgtcgga atgcctgccc
     3181 tactacgtag agctgttccg gcagatgaaa agccatgccc tggccgcaaa aaccattccc
     3241 aaaacgacgg tcagcggatc ccgctagcaa aggctgtggc gcgcggcccg gacactgacg
     3301 aaaactccgc ctgaggctaa ggttcagctc aacagcgacg cggagggtgg cccggtccgg
     3361 atgaaaaccg aattcacgct caatcagaaa cgcgccctcg ccgtcctgac tctgatcgca
     3421 ttgctgttcg ccggatattt cttgcgcgac tttttcgtcc tcattgtggt ggccgccgtc
     3481 ggggcgtatt tgttcacgcc agttttcaat cggttcaaca ggcgactcgg taccggcctg
     3541 tcggccacct gcaccctgtt gacggcgctg gcgatagtcg cagtcccgat cgcactcctg
     3601 atcgtgttgg caatcgtcca gatttcgcgc atgatcgaca gcgtcgcggg atgggtcaag
     3661 accaccgacc tgagcgggct cggcgataaa gcgttacacc tggtcaacga attcgcggcc
     3721 cgaatcccct tcgtgcacat aaccgtaact gccgagatgc tgcgcaaggc gatggtcacc
     3781 ggcgcgcaga acgtcggtca gtggctgctg catttcctgc agggcgcggt cggaggcatc
     3841 gccggcgcca tcacggcggc catcatcttt ctgtacgtat ttctcgcgct gctggtgaac
     3901 cgcgagaagg tccgcacgtt gatcggccag ctcaatccgc tcggcgacga agtcaccgac
     3961 ttgtacctga cgaaggccgg cgccatggtg cgcggcaccg tgaacggcca gttcgtcatc
     4021 gcgctgtgcc aaggtgtctc gggcgccgcg tccatctaca tcggcggatt tcaccacggt
     4081 ttcttcatct tcgcggtgct gctgacggcg ttgtcgatca ttccgctggg cagcggtgtc
     4141 gtgacgattc cgttcggcat cgggatgatc ctctacggca acgtcgttgg cggcgcgttc
     4201 gtggtgctgt tccatctggt tggggtcacg aatatcgaca acttcttgcg ccccatcctc
     4261 gttccgaaag acgcacgcct gaactcggcg ctcatgctgc tttcggtgtt cgcgggcatc
     4321 gccatgttcg gtccctgggg catcgtgatc gggccggtgc tcatgatcct gatcgtcacc
     4381 accatcgacg tctaccttgc ggtctacaaa ggcgtcgaac tccaggcgcc cgatgaagaa
     4441 cccgtgcacc gcaattggtt cccgcgccgc aaagcgaatg aaagcgcccg ggaagccggg
     4501 gaagccgggg aatccaagga atccgagaaa tcggaggccg actgatcgcc cgggccgact
     4561 accggaaact caccacctcg ctgccccagg cggctcggat gtgcccgtcg acgtcgcgca
     4621 cgacccggtc gcgcttgtcg atgatcaggc tggcacggtc gaccccccgg tacggcgccc
     4681 agtccggctc gcccgccggg ccggcgggct ttccctgcgc cgcgaagttg acccaccggg
     4741 tccgcactcg cttcgagacc gccttggccg ttttggcgcc accgagcttc aacgtgggat
     4801 ccctgggtgc cccgagattg ccccagacat aaggcaattc ggtggcgtgg gcggggccgg
     4861 tgagcagcag cttccagaac ggagtcgcgt agtcgaaccg gtagagatac accggcgcca
     4921 ccctgctgtg accttcggcc agccacaccg aaggcatccg gaatccgaga tcggtggcga
     4981 tgcccaaagg ccttgccttg ccccgcattc gcgaatacgc cgagccgatc tgctgctcgg
     5041 tgggcaattg caggtcgggt tgttcggcgg cgatctgggc gaacatcgcc ttgatcgcgt
     5101 gcggcgtgat cggcatcagg tgcgacctca tcagtcggaa cagcgccgcc tcacgcttgt
     5161 tggtgccgat gatcaacggc accgggtggg agcagccctc ctgcaccagc ttgaccgggt
     5221 agtcgtgcag cagttcgccg tcgacgatcg ggacgaacgc aagcgtgccc ggattgcgcg
     5281 cgggcacttc gtcgaacgcc tgttgcgacg cggccagcat cgccgcggtc ggcacgtcga
     5341 gcaccctgtc gacgtcggac ggctcgattc ccagcttgcc gaggatgccc accgcgacgc
     5401 gccgagccct gtcccggtcg tacaccccgg tggccggtga gctctgggcg atggcgccgg
     5461 cgaacaggcc ctcggccgcc ggggtggcga gcagggtcgt gacgatcccc gcgccggcgg
     5521 actcgccgaa cagcgtgacc ttgtggggat cgccgccaaa cgccgcgatg ttgtcgcgca
     5581 cccagcgcaa cgcctccagc acgtcgtgca gcccgatgtt cgagtcgaag cgtcgccgcg
     5641 ccgtgttgaa cgacgagagg tcgaggaagc ccagtgctcc gagccggtag ttgactgtga
     5701 cgacgaccac cccggcgtca ctgtcgccgc cggcggccaa cctgcgaccg tcgtagagcg
     5761 cctggctgcc ggatcccagg atgtaggcgc cgccgtgcag ccacaccatc accggtttcc
     5821 ggtcgccggg ctgggtgtcg gacgccgccc agatgttcag ccgcaggcag tcttcgccct
     5881 gcggagcccc caggtcgagt ggcatgttgg gaaaggtcgg ttgcgggcag gccggcccga
     5941 aggcggtggc gtcggcgacc tgggtccacg gcctcggagg ctcgggtacc cggaaacgca
     6001 ggtcgccgac gggcggtgcg gcgtagcgaa tgcccttcca cgtcttgacg cggccccccg
     6061 ccaagcccgc gtcgctgccg cgcaccgggc cgtaggtggt gtcgaccaca cactcgtcga
     6121 tcgccatacg tcgtgaaact ccctaactga gccgtgcggt caggaactcg actacccgtt
     6181 tgcgggcctc gtaagcgggc tggccgtcga cttcacgcac ctcttcggtc agcaccgagt
     6241 gcgcgctctt gtcgaaacct cccgcgttgc ccgggccgga gtcgatctca atcacctcaa
     6301 acgcgtcgcc gagccgcgcc ttgagcgtcg cgaaccgttc ccgcggcgcc ttccagtcct
     6361 cgctgaaccg caaaccgagg gcgcacagcc cgtcgttagc acagcgatcg gcgacggtgg
     6421 ccagctcgga ttcgctcagc cccggatcac ggcgccgcgc atcgcccagc gggaatggtg
     6481 ccgacggctg gctgagcacc ggggccagca cgctgtcgtc gaccgcggcg gccaacgcga
     6541 agccgccggt gaagcactgg ccgatcacac ccacgccctt gcccggagtc gacgcgttga
     6601 ggtcacgggc cagtccacgc aggaacaacg acaccggccg ctgcctgttg gtggcgaacg
     6661 ccgcgaattc ccttgccaca caagcccgcg cgatggccgc ggtgacgtag cggttcgtca
     6721 cgggcttggc cggttcaccg aacaacgagg gaatggcaac ggtgaagccg ttgtccacca
     6781 ggtggtcacc gaggcccagc acaccgggag tgatccccgg tagctcgggg atcagcagca
     6841 cgccggggcc ggcccccttg cggtacacat cgtgggtgta gccaccgtcg ctgaacggtg
     6901 ccgccgacca tccggacagg tctgcttcgg gtgcggtcac gctgcgtccc ctctatcggc
     6961 gtgctgacta gcgactcgcc aagggcggct gggattgcgt cgccgccgcc agcgtacgga
     7021 actggtcggt gctgccggca ccggtgatcg ctatctgcgt ggtgccggcc gggccggtga
     7081 gcctggtggt ccacaccggc tcggcgtcgg cgccggcttg gccggcaccg cgatagacca
     7141 cccagctggt gccgtcgacg tcgaccgttc cgatcggata agccgcggga tggatcgagc
     7201 cgaccagctt gtcctcgtcg gcgttgctct gggtcaggct cagatacatg ccggtcgggc
     7261 tgatgaatcc cacgattgag gtggccgcgt tcagccgccg accggtcgag gggtcggttc
     7321 ggccgttctc gatgccaccg cggccgccgg agttgggccg ccagccggcc ggcaactgag
     7381 gcaaccgaac ggggaacccg agcgtctggg cgtccgcccg cagggccgcc gtcgcgtcgt
     7441 aggacggaat caccgaattg ttcgtccggc cgggctggaa cgaacacatc ccgaccaggc
     7501 ccgccagcag gatgcacccg accaccagcg gcaccagcga ccagaacatg tcgcgaccgt
     7561 cctgcagcag ccgcggtttg gcgggcttcg gaacgggcca ccccgccgga gccggctcgc
     7621 ctacctgggc attcggccgt gcctcctcgg tcatcccccg agtattccag ccggccgtca
     7681 ccagccacag cccagctcca gcgcaccgat tggtttctgg gacaatcagc aaccatgaca
     7741 gctgaggggg accgcccatt gggaaccggt tcgtcgccga ccgccaccgc ggaccgcaac
     7801 ccggcacagg tgcggccacg cggtgaagcc ccggaccgca acctggccct ggaactggta
     7861 cgggtcaccg aggccggtgc catggccgcc ggccgctggg tgggccgcgg cgacaaagag
     7921 ggcggcgacg gcgcagcggt cgacgcgatg cgcgaactgg tgaactcggt gtcgatgcgc
     7981 ggggtggtgg tgatcggcga gggcgagaag gaccaggcgc cgatgctcta caacggcgaa
     8041 gaggtcggca acggagacgg gcccgagtgc gatttcgccg tcgatccgat tgacggcacc
     8101 accttgatga gcaagggcat gcccaacgcc atttcggtgc tggcggtggc cgatcgcggg
     8161 gcgatgttcg acccgtcggc ggtgttctac atgaacaaga tcgcggtcgg gcccgaggcc
     8221 gcccacgtgc tggacatcac cgcgccgatc gccgagaaca tccgggccgt cgccaaggtc
     8281 aaggcgttgt cggtccgcga catgacggtg tgcatcttgg accggccgcg gcacgcccaa
     8341 ctgatcgagg acgtccgggc aacgggagcc cggatccggc tgatcaccga cggcgacgtc
     8401 gccggcgcca tctcggcgtg ccgcccggag tcgggcaccg acctgctcgc cggcatcggc
     8461 ggcaccccgg agggcatcat cgccgcggcg gcgatccggt gcatgggcgg cgcgatccag
     8521 gcacaactgg cgcctcgcga cgaggccgaa cgccgcaagg cgctcgctgc cggctacgac
     8581 ctcgaccagg tgctgaccac cgaggatttg gtgtccggtg agaacgtctt cttctgcgcc
     8641 accggggtca ccgaaggtga cctgctcaag ggggtgcggt attaccctgg cggctgcacc
     8701 acccactcga tcgtgatgcg ttcgaaatcg ggcaccgtcc gcatgatcga ggcctaccac
     8761 cggctctcca agctcaacga atattccgcc atcgacttca ccggcgacag ctctgccgcc
     8821 tatcccctgc cctgaccaca tcccatcgaa caaggaagcc catcaatggc cgctgacggc
     8881 tcccagaccg acaccgagta ccgcatcgag cacgacacca tgggcgaggt ccgggtaccc
     8941 gccaaggctc tgtggcgcgc gcaaacccag cgcgcggtgg agaacttccc gatctcgggc
     9001 cgtggcctgg agcgcaccca gatccgggcg ttgggcctgc tgaagggcgc ctgcgcgcag
     9061 gtcaacaagg atctgggcct gttggcgccg gagaaagcgg acgcgatcat cgccgccgcc
     9121 gccgagatcg ccgacggccg ccacgacgac cagttcccca tcgacgtctt ccagaccggt
     9181 tcgggcacca gctccaacat gaacaccaac gaggtgatcg ccagcatcgc ggccgccaac
     9241 ggggtggcgg tgcatcccaa cgacgacgtc aacatgtcgc agtcgtccaa cgacaccttc
     9301 cccaccgcca cccacatcgc ggccaccgag gccgcggtgc gtcacctcat ccccgcgctg
     9361 cgggagctgc acgacgccct ggccgccaag gcccacgagt ggcacacggt ggtgaagtcc
     9421 ggccgcaccc acctgatgga cgcggttccg gtgacgctgg gccaggagtt cagcggatac
     9481 gcccgccaga tcgaggccgg gatcgagcgg gtgagtgcca ctctgccccg gctgggtgag
     9541 ctggcgatcg gcggcaccgc ggtgggcacc ggcctcaacg cccccgacgg cttcggaggc
     9601 aaggtggtcg agacgctggt cgccgaaacc ggcttgtccg aattacgcac ggcgtcaaac
     9661 tctttcgagg cccaggccgc ccgcgacggg ctggtcgagg cgtccggtgc gctgcgcacc
     9721 atcgcggtgt cactgaccaa gatcgccaac gacatccgct ggatgggctc cggccccctg
     9781 accggccttg ccgagatcca gctgcctgac ctgcagccgg gtagctcgat catgccgggc
     9841 aaggtgaatc cggttctgcc ggaagcggtt acgcaggtgg ccgcgcaggt gatcggcaac
     9901 gatgccgcgg tcgccgtcgg cggcttgtcc ggcgcgttcg agctcaacgt ctacatcccg
     9961 atgatggccc gcaacattct cgagtcgttc aagttgctga ccaatgtgtc aaagctgttc
    10021 gcgcagcgtt gcattgtcgg gctgaaggcc aacgtcgagc acctgcgcga actggccgaa
    10081 tcgtcgccgt cgatcgtgac gccgctgaac tcggccatcg gctacgagga ggccgcggcg
    10141 gtggccaagc aggcgctcaa ggagcgcaag accatccgcc aaaccgtgat cgaccgcggc
    10201 ctgatcggcg acaagctgtc gatcgaagag ctggaccgcc gcctcgacgt gttggctatg
    10261 gccaaagtca agccggaaga ctagtggcga tcgcaagcgc ggcggtggag gtacctctcg
    10321 taagcgcggc gaagccgggc gcagcgggtc gccatcatgg tgatcagtgg cgatcgcaag
    10381 cgcggcggag tcgagggaag cgggtcgcca ccattaggat cagcccgact cgctgcgaag
    10441 gcggtcgatg aaggtgaggg tgatccagca atgacggctc ctcctggcgg tccctacaat
    10501 caggatccct acggggcgaa tccctatggc caggggccct attggggtgg ccagccgcag
    10561 ggcggctcct acccgtaccc gccgccgggc agcccttacg cgcatcctca cggagccgcg
    10621 gatccctacg cggcgggccc gtacccaccc cagcagtacc cccagccggg ccagcaagtc
    10681 ccccccggct ggccgccgcc gggcgggtac ccgcccggac cgccgccgaa ggggccgcgg
    10741 tccaaagcgc cgtgggtgat cctcgcggcc gtcgcagtgc tgggcgtcat cgcgctggtg
    10801 atcatcctcg tgatcggcct caacgaccga aacaagccgt ccagcgccaa cccctcgggc
    10861 ccggcgggcc ccgcgccgac gacgacaggc gccccgacgt cgcagccgaa caacgccggt
    10921 cagacggcga ccggctgcac gcccaacgtg tccggcggtg acaagcccgt cggcgacacg
    10981 gtcagcgcgg gcaaactgtc cttcgcggcc agtgcggcgc ccggctggtc gggcttctcc
    11041 gatgaccaga ccccgaacct gatccaggcc gtgggcatcg ggcaagaggt gcccaccgcc
    11101 agccagtgga tgatgcaggc cgaggtcgcc gtcaccaact tcgtcgccag catggacgtc
    11161 ggcgcgcaag catcgaaatt gatggactgc gtggccaacg gccccggcta cgcgaacgcg
    11221 acgccgaccc tgggccccat gaagacgtcg tcgatcacgg tcgacggggt caacgccgcc
    11281 cgggtggacg ccgacgtcgc gatcgccgac accgcccgca acgtcaaggg cgactccctc
    11341 gtcatcatcg ctgtcaacac caagccggtc accatcttct tgggtgcgac acccatcggc
    11401 gatgcgggct cggcgaacat catcaaccag gtcatcgcgt cgctgaaggt cagcaagaag
    11461 tgacgtgtcc tgttgtccgc caagctattt gggggctagc cggcgccacc ggcgccgaaa
    11521 cgtgggtggc ctccgaacgc ccgcgcaaca cggtggagta gcactcggcc cagcgcgccc
    11581 gctcggcctc gtcggctcgg tcgacggccg ccgccgagca caggatccgc cggtcggcgg
    11641 ttttagcgag gtcggccagt cgggcggctt cgttgaccgc gtcaccgatt accgtgtatt
    11701 cgtaacggtt ttcggcaccg atgttgccgg cgaacacccg gcccgccgag acgccgatgc
    11761 cgaaatccac cacgggcaac cggcgcaatt gggtgcctaa cgcacgggcc gtcatcagcg
    11821 ccgccgagga gggtttagtc ggttcgctca tccgcagcgg cgccccgaag acggccagtg
    11881 cggcgtcgcc ggcgaatttg ttgatcagcc cgtgccgttc gtcgacggcg ccgacgacga
    11941 tccggaaaaa gtcgttgagc acctcggcaa cctcttgcgg ggggcggctt tccgcgagtt
    12001 gtgtcgaacc caccaggtcg atgaaaagga ccgccgcttc gaccacatct ccggacagcg
    12061 aggcgccctc ctggatcgcg cgctgcgcga cgtccgcgcc gacgtggcgc ccgaacaggt
    12121 cccgcaaccg gtcccgttcc gcgagccccg ccaccattcg gttgaatccc gtttgcagac
    12181 gcccgatttg ggaacgctcg tacgcgccga cggatgtgcc gatgcggccg tgctcgacct
    12241 cggccatcgc gtcgacgacc tcaccgaccg gatcggaaat cgatcgcgac gtcaggatca
    12301 tcgtcggcag cccgagcagc agcgccgcca gcgacaccac cagaacgggc acatccagcg
    12361 acgccgactt ctgaatcagc cacccgtagg agcgaagaac gaccagggcc gcgatcaccc
    12421 cgatgggaat tgcgctgcac aagaaccaca gcaggaccat tcgggcgaac acacccggta
    12481 ccgcgagccg gggctcgcat cccagggtcg cgacacccat gatgggacgc agggtgcgct
    12541 gcgcgagcag catcccgata cccgcggcgg cgggcccgcc cagcagcacg ccgagcagga
    12601 tcggcagcag cagctggggc ccgccgtggc gattcagcag tgcgaagaat gccccggcca
    12661 cgctccaggc cagcacgagg atcgccgact gacggcccgg gatcttcatt gcgatttccc
    12721 gttgtgcagc agtcggttcg tccccggcga cataccaacg cagcgtgggg gccaaggtaa
    12781 cgacgccggc gatcaccacg ccgacggtcc ccaacgcgac gaacgcgatc accaccgccg
    12841 tgttcttctg cgcgaagtcg gcgttggccg ccacggacgt gtggccccgc agcgggatca
    12901 agatcgcggc cgcgtcgacg acggccagca ggtaggcgag ggcgaggtcg atcgcatagc
    12961 gaatcgacaa cctgcgagcg gactttggtt gccctgaccc ggataccgcc gccggaccgc
    13021 gcgatcgcgg cggggcacca gcccgaagcc agcccgcctt gatcacccta taaaggtagc
    13081 tgtgcgcccg ctcagggttg tgctacgcac cgttgtttgg gccgcccgag ttctgacccg
    13141 gaatgtcggt gatcgggaaa ttgggcatcg gcttgggtga gggggtgttg ggcagggttg
    13201 ggatctcacc gggcgggatg tcgtgcagcg cgttgacggg tggcccgttt tcccggccgc
    13261 cgtagctgct gccgggcgcg cgcgacccca gcaattcgct gatcgtcacc aggcggtagc
    13321 cgttggcctt gagcaccgga atgaattggt acaccaggtc gacggtgctc gagtaggtat
    13381 cgtgaaacaa caccaccgag ccgggcttga tgtatgtcat cagcatgtag cgggtcgcgg
    13441 ctgtgttgga atcgttgatc cagtcgaaag gtattacatc ccagaggatt tcgccaagtc
    13501 cgaacttggc ggcggtctgc cgcaccacgt cgtccgacag gccgccggcc ggtcgccaca
    13561 gcgacggcgt ccggccggtc gcggcggcta tcgcgtcgtt ggccctggag aactgagcgg
    13621 cgacgtcgtc gacggggatc gtcgtcatat tggggtgctc ccacgtgtgg ctgccgatct
    13681 ccatgccggc gtcggcgatg cgcttggcgc cggccgggtt ggcggccacc ttgttgccga
    13741 tcaggaagaa ggtcgccttg gcgtcgctgt ccttcaggat ttgcagcaac cggtcggtgt
    13801 atggccccgg cccatcgtcg aatgtcagcg cgatgcactt gaccactgag cagccggggt
    13861 tgtcggcgcg cgtcacgtga ccggtaaggc cgccgatcac caggaccgcg gcggccgcga
    13921 cgacaccgaa aacggtccgc caatagcgcc aggcctggct gtcgggtctt ttcggcacgt
    13981 aggcagttta tcgaacgtga cgtgcacgac ctcggcgtcg agtgcgcagt gcgggctttc
    14041 agtgttacct cagggcggga ttcgcgcgat tttctcgccc tggctgcaca cgcaatgcca
    14101 aggactcaca ctcaacgccg gggaatcaca ctcactgcgg tccggcgatt tcctcgagca
    14161 tctcggtgac cagcgcggcg atcggtgacc gctcgctgcg cagcagcgtg atgtgggcga
    14221 acagcggatg acccttgagc ttttcgatca cggcggcgac cccgtcgtgg cggccgacgc
    14281 gcaggttgtc acgttgagcg atgtcgtggg tcagcaccac ccgcgatccg gcacccaatc
    14341 tggacaacac ggtcagcagc acgttgcgct ccagcgactg cgcctcatcg acgatgacga
    14401 acgaatcgtg cagcgagcga ccccggatgt gggtcagcgg caacacctcg agcatgcccc
    14461 gggaaagcac ttcctcgagc accgccgggc tggccaggcc ctcgagggtg tcgaagacgg
    14521 cctgcgccca cgggcccatc ttttcgctct cgctaccggg caggtagccc agctcctggc
    14581 cgccgacggc atacagcgga cggaagacca ccaccttgcg gtgagtacgt cgttccagca
    14641 ccgcctcgag acccgcgcac agcgccagcg ccgacttgcc ggtgccggct ttaccaccca
    14701 gcgacaggat gcccaccgac tcgtccagca gcaggtcgag cgccacccgt tgctccgcgg
    14761 aacggccgcg caacccgaac acctcgcggt caccgcggac cagctggacg cgcttggcgg
    14821 gggtgacccg gcccagcgcg tgcgacccgc cgcccagcaa ccgaatcccg gtgtggcagg
    14881 gcaggtcccg ggcctcggcc aggtcgatct caccgtcggc gaacagcgcg tcgatatcct
    14941 cggccccggt ctcgatctcc tgcatgcccg accacccgga gaccacgaca tcctgcgcgt
    15001 ggtactcgtc ggcggccaga cctaccgcgg ccgccttcac ccgcagggga atgtccttgc
    15061 tgaccaacgt gacccgcttg ccctcggcgg cgaggttggc ggcgcaactc aaaatccggg
    15121 agtcgttgct gtcggtgcgg aagccggccg gcagcaccgc cgggtccgtg tgattgagct
    15181 cgacgtgcag cgttccgcct tgtgtcccaa cgggaatggg ctgatccagc cgtgagtgct
    15241 ctagccgcag atcatcgaac aggcgcaacg cctgacgggc gaaccacccc agctcgtgat
    15301 ggtggcgttt ggcctccagt tcgctgatca ccaccagtgg aacaaccacc tcgtgttcgg
    15361 cgaaccggct gcacgcccag gggtcggaca gcagcacgga ggtgtcgagc acataggtcc
    15421 ggatttcggt cacgaagcgc tcctcgagcg ggtaagcccg cgcggacccg cacaggcatg
    15481 tcggcgggag ccgcagcacc aggaccgggg ccggtcctgc ccttgtagtg acgggaggtg
    15541 ccgccctggc cgcaaagcat gtcgctggcc atcgggaacg acgctactct cgtggccgct
    15601 cggccgccgc gcaggcgcgc cgtgaccccg atggtgaccc ggaatgcggg cgaccgaggc
    15661 g
//
LOCUS       DASSGQ010000159         8872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1469719,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000159 DASSGQ010000000
VERSION     DASSGQ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469719"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VFI55_07145"
     CDS             <1..809
                     /locus_tag="VFI55_07145"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="AcsA; in Sinorhizobium meliloti this enzyme is
                     required for acetoacetate activation; similar to
                     acetyl-CoA synthase; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HET7073841.1"
                     /translation="TFEWCYRNIKDDLWVTSQSGGTEICSGFVGASPTLPVYAGEIQA
                     RLLGMDVHAWNDDGAEVLDEVGELVVTTPFPSMPIRFWNDEGDKRYRESYFETFPGVW
                     RHGDFLKINSHGGCYIYGRSDSTLNRYGVRIGTAEIYRAVEQEPEVADSLIVCCELPG
                     GNFFMPLFVKLKPGMSLTDEVRARLNSRLRRDCSPRHVPDKAYVVADVPYTLTGKKME
                     VPVRKILMGWPVDKAVSRDAMANPESIDFFIEFARTSEDYTLHVEPEVSE"
     gene            806..2545
                     /locus_tag="VFI55_07150"
     CDS             806..2545
                     /locus_tag="VFI55_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7073842.1"
                     /translation="MTTQATRTPPKTARRDGGPPSAERVVDNVLGQQVMGDFRPSDLF
                     GTASTMAAGVVRRPTALVRASLGFLGELGLLAAGESHLEPKPGDRRFTDPIWQENVVY
                     TAILQSYLALSNSLTRFAETSSTNVRQSERNRFLLMQIADAIAPTNFLLGNPAALRRA
                     WETRGLSLLRGGRNFLADVRSRRPVPSQVDGSAFEVGVNLACSPGSVVLRTEMFELIQ
                     YAPRTAKVRQRPILIVPSIVNKFYVFDLAPGRSIIEYFVQNGLTVFTMAWRNPHKRHD
                     NWGMTDYQDAIDTAIDATRKISGADSVNLWAVCGAGPVAVSLAGYHAATDQRKINSLL
                     LVVSPLDTQAMSDAPAIGAFIDKDSPAAPRPVLKAMRRRRMSAREFALVFAMLRPNDL
                     IWNYWVSDYLMGNSPTAFDVLYWNEDGTGMTAQFNHDFSEFLEQNPFVTTGAMRVRGT
                     PIADLSELGFDSYVLGAKTDHLCIWQGVYRSAQLLGPRSQFVLGNSGHIQTIVCPPDN
                     PKASFNTNEGVSQSAADWLATSQRNSGSWWPHGVAWTRERSGPLVKASQKAGDSEHPV
                     LCDAPGTYVRERI"
     gene            2593..2715
                     /locus_tag="VFI55_07155"
     CDS             2593..2715
                     /locus_tag="VFI55_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073843.1"
                     /translation="MESLEKGTRWGTAELRLSLPLRCTFYISGQTVVCSGGYMF"
     gene            complement(2871..4163)
                     /locus_tag="VFI55_07160"
     CDS             complement(2871..4163)
                     /locus_tag="VFI55_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7073844.1"
                     /translation="MGDGALPLVLVPGWVSNVDLYDDPAYPFAPLVERLAGQTRLILW
                     DKRGTGLSDPVSRVPTLDERMDDLRAVLDAAEVDCPALLGISEGGPMSILFAATFPDH
                     VRSLVLYGTAARFTQELPDFPWGWSPEQVGRGVEIYDKHWGEGVFATLVRGSSTEVPG
                     LRELFGRYERASASPAMAAMLMKAATEIDVRKILPTVKTNTLVLARPGDRVVAFEASE
                     ALAAAMPNAVFRALPPGAHGDFDIDDSLATETLTFLCGPLSSPTSERVLATVLFTDIV
                     GSTEQLSSQGDSRWRQQLDVHDQLVDRSLSKYGGRRAKHTGDGVFALFEGPTKAARCG
                     LELVPALATRGIRIRVGIHTGECERRGEEWSGVAVHVGARIGAMAGPDEVLTSRTVRD
                     LSAGSGLRFESLGAHRLKGLAEETEVFRVGTPTSGLTP"
     gene            4724..6109
                     /locus_tag="VFI55_07165"
     CDS             4724..6109
                     /locus_tag="VFI55_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015345094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HET7073845.1"
                     /translation="MSFVIAVPEYVTAAASDLANVGSTITAANAVAAVPTSTVLAAGG
                     DEVSAAVAAIFGAHAQAYQELSAQAATFHQQFVQLLNAGAGSYANAEAANATPLQSTQ
                     QDLLNAVNGPTEAILGRPLIGDGANGINGTGASGQNGGLLWGNGGAGGSGAAGQNGGN
                     GGSGGFLFGNGGPGGPGGAAIGNGAAGFGGDGGNAVGLFGSGGIGGTGGNAGTLGVGG
                     DGGFGGSGGILLGNGGIGGAGGTGGVAGWGGDGGDGAGMLYGLGGAGGAGGATTNAVG
                     FAGVGGNGGSGNILFNLISTGADGGAGGAAVGGANIGGQGGQGGGGAGELWGIGGNGG
                     VGGAAVTSGGTGGPGGYGGDGGAFFGMGGAGGVGGAAIGNGHGGVGGLGGLGGLLFGI
                     GGAGGDGYGAPGLAFGGNGSQGGYGGLLFGIGGPGGNGGIGVTPGIGGPGGFGGYYLF
                     GPNGPSGLTPA"
     gene            6372..6758
                     /locus_tag="VFI55_07170"
     CDS             6372..6758
                     /locus_tag="VFI55_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073846.1"
                     /translation="MAEESSAAFLDRVKELLTQNEARMKTVQEQIASQWDTQHKAISD
                     LVEGQWNAQQKAISDLIEQGQDQTAKIADLWESRRKAIADQVEQQWNAQQKSISDLIE
                     TQRNAQKKAIEDVHSALGGVLGDKSE"
     gene            complement(6907..7251)
                     /locus_tag="VFI55_07175"
     CDS             complement(6907..7251)
                     /locus_tag="VFI55_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HET7073847.1"
                     /translation="MAAERLSGRLIRAALVGVPLLIAGVVCAGPAHADEASYLTHLHN
                     IGIQDLDGGDPALVQTGWKICQQLTWGAPPQQLQSLALQSSVQDLGPRGLTPRQADAL
                     IDYALVDLCPRA"
     gene            7525..8694
                     /locus_tag="VFI55_07180"
     CDS             7525..8694
                     /locus_tag="VFI55_07180"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET7073848.1"
                     /translation="MLKYAAAAPVVLGLGMAADVLIGPARVAADPYLSAAAMAPGQPV
                     NIISRAGWGANESIRRGGRLCDNGIRAGVVHHSATGNDYAPTDSAAIVRSIYEYHTLT
                     QGWGDIGYNALVDKYGQVFEGRFGGITNPVEGVHTGGFNANTWGVCMIGDFSQAPPTP
                     DQLRAVGQLLGWRLAMDAVNPKGTVALTSAGGPNTRFPRGAAETLPSIFGHRDVGDTD
                     CPGNAGYAFLERIRDIAARFKQPPSAQELAESLKGGAIYDRWQATGGMAGGLGAPTSP
                     EAACGDSIRYVVFASGAIYWSPATGPQPLTGRIYEAWATLRYERGPLGLPTSAEIHEP
                     EWIVQNFQHGTLNFNRQTSTVMSVIDGVATVVPPPPAGGPPVQLERFSAARNPVV"
BASE COUNT         1562 a         2824 c         2958 g         1528 t
ORIGIN      
        1 agaccttcga atggtgctac cgcaatatca aagacgacct gtgggtgacc tcgcagtccg
       61 gtggcacgga gatttgcagc ggattcgtcg gagcgtcgcc gacgctgccg gtctatgccg
      121 gcgagattca agcccgattg ctcggcatgg acgtccacgc gtggaacgac gacggcgccg
      181 aggtactcga cgaggtcggc gaactcgtcg tgaccacgcc gttcccatcc atgccgatcc
      241 ggttctggaa tgatgaaggc gacaaacggt atcgagagtc ctacttcgag acgtttcccg
      301 gcgtgtggcg ccacggcgac ttcctcaaga tcaattccca tggcggctgc tacatctacg
      361 gccgatcgga ctccacactc aaccggtacg gcgtgcgcat cggcaccgcc gaaatttacc
      421 gcgcggtcga acaagaaccc gaggtcgccg acagtttgat cgtgtgttgc gaattgccgg
      481 gcggaaactt cttcatgccg ctgtttgtca aactcaaacc cgggatgtcg ttaaccgacg
      541 aggtccgtgc acggctgaac tctcgtttgc ggcgggactg cagtccgcgt catgtgcccg
      601 ataaggccta tgtggtggcc gacgtcccgt acacgctgac cggcaagaaa atggaagtcc
      661 ctgtccgcaa gatcctgatg ggctggccgg tggacaaggc cgtcagccgc gacgcgatgg
      721 ccaaccctga gtcgatcgac ttcttcatcg aattcgcccg tacctcagag gattacaccc
      781 tgcacgtcga gccggaggtc agcgaatgac aacacaagca acgcgaaccc cgccgaagac
      841 tgctcgtcgc gacggcggtc cgcccagtgc cgagcgtgtc gtcgacaacg tgctcgggca
      901 gcaagtgatg ggcgactttc gccccagcga cttgtttgga accgccagca ccatggccgc
      961 tggcgtcgtt cgacgcccca ccgcgttggt gcgcgcctca ttgggttttc tcggtgaact
     1021 cggtctgctc gcggccggcg agtcgcatct ggaacctaag cccggcgatc ggcgcttcac
     1081 cgacccgatc tggcaggaaa acgtcgtcta cacggcgata ttgcaaagtt acctcgcgct
     1141 gagcaattcg ctgacgcgat tcgccgaaac gtcgagcacc aatgtccggc agtcggaacg
     1201 aaaccgcttc ctgctcatgc agatcgccga tgcgatcgcg ccaaccaact ttttgcttgg
     1261 caatcccgct gcgttgcgca gggcctggga aacacgcggg ctgagtctgc tgcgcggcgg
     1321 gcgcaacttc ctggcggacg tccgcagccg ccgaccggtt ccgtcccagg tcgacgggtc
     1381 ggcgtttgag gtaggcgtca acctggcgtg ttctccggga tcggtcgtgc tgcgcacgga
     1441 gatgttcgaa ctcattcagt acgcgccgag aaccgccaaa gtacgacagc gaccgattct
     1501 catcgtgccg tcgattgtca acaagttcta cgtgttcgat ctcgcgcctg gacgcagcat
     1561 catcgagtac ttcgtgcaaa acggcctgac cgtcttcacg atggcgtggc gcaatcctca
     1621 taagcgccac gataattggg ggatgacgga ctaccaggac gccattgaca ccgcgatcga
     1681 tgcgacccgc aagatcagcg gagccgatag cgtcaacttg tgggcggtct gcggagctgg
     1741 ccccgtggcc gtttccctgg ccggatatca cgcggccacc gatcaacgga agatcaactc
     1801 actgctgctt gtggtctcac ccttggacac gcaggcgatg tcggacgcgc ccgctatcgg
     1861 tgccttcatc gacaaagaca gtcccgcggc acccaggccg gtcctcaagg cgatgcgcag
     1921 gcggcggatg agcgcacgcg aattcgccct ggtcttcgcg atgttgcggc ccaacgacct
     1981 gatctggaat tactgggtga gcgactatct gatggggaac tcgcctaccg cgttcgacgt
     2041 gctgtactgg aacgaggacg gcaccggtat gaccgcccag ttcaaccacg atttcagcga
     2101 gttcctcgaa cagaatccgt tcgtgaccac cggagcgatg cgggtgcgcg gcactccgat
     2161 cgctgatctt tctgaactcg gtttcgacag ttacgttctc ggtgccaaga ccgaccattt
     2221 gtgcatctgg cagggcgtgt accgaagcgc gcaactcttg gggccgcgca gccaattcgt
     2281 tctcggtaac agcggtcaca tccagacgat cgtctgccca cccgacaatc ccaaggccag
     2341 tttcaacacg aacgagggtg tgtcgcaaag cgcagcggac tggttggcga cgtcgcaacg
     2401 caattctggc agttggtggc cgcacggtgt cgcctggaca cgggaacgat ccgggccgct
     2461 cgtcaaagcg tcccaaaaag ccggcgacag tgagcatccc gtcctgtgtg atgcccctgg
     2521 gacttatgta cgcgagcgga tatgaccatc gcagacccat ccaagctaac gtcagcgccc
     2581 gagccgggcc gcttggaatc tctcgagaag gggacccgtt ggggcacggc ggaactccgg
     2641 ctgagcctgc cgctgcggtg taccttctac atcagcggcc agacggtggt gtgcagcggt
     2701 ggctacatgt tctaaccgct ccctccaccg tctcttaatc cattgcattc caaagagatt
     2761 cacggcacca cggccgccgc ggcgggtatg gtgcagccgg accgtctggg ctgcgctcga
     2821 cgcaatggcg aagcgtgccg aagcccactt acggcggcaa cgtggtagcc ctacggcgtt
     2881 aggccgctcg ttggtgtacc gactcggaag acttcggttt cctcggcaag gccctttagg
     2941 cgatgggcgc caaggctttc aaagcgcagg cctgagcccg cggaaagatc acgcaccgtg
     3001 cgactggtaa gcacttcgtc gggtccggcc atcgcgccga tccgtgcgcc gacgtgaacg
     3061 gccacgccgc tccactcctc cccgcgtcgt tcgcattcac cggtgtggat gcctacgcgg
     3121 atgcggattc cgcgcgtcgc aagtgcgggt accagctcca ggccgcatcg cgctgccttg
     3181 gtggggcctt caaataacgc gaacactcca tcgccggtgt gtttggcgcg tcggccgccg
     3241 tactttgaca acgatcgatc gaccaactgg tcgtggacgt cgagctgttg ccgccaccgc
     3301 gaatctcctt ggctgctcag ctgttcggtg gagccgacga tgtcggtgaa caacaccgtc
     3361 gcgagcacgc gttcgctggt cggactgctc agaggtccac agaggaaggt gagcgtttcc
     3421 gtggcgagcg aatcgtcgat gtcgaaatcg ccatgagcgc ctggcggaag tgcccggaac
     3481 acggcattgg gcattgcggc ggccaacgcc tctgatgctt caaatgcgac gactcgatcg
     3541 ccgggtctgg cgagcacaag cgtattggtc ttcacggtgg gcaagatttt ccgcacatcg
     3601 atttcggtag ctgccttcat caacattgcc gccatggccg ggctcgcgct agcgcgttca
     3661 tatctgccga acagttcacg gagaccgggc acctcggtgg aagagcctcg cacgagtgtc
     3721 gcgaacacgc cttcacccca atgtttgtcg taaatttcga ctcctcgacc gacctgctca
     3781 ggtgaccagc cccaggggaa gtcgggtaat tcctgcgtga accgcgcggc tgtgccatag
     3841 agaactagcg accggacgtg gtcgggaaat gtcgcggcga acagaatgct catcggccca
     3901 ccctctgata tgccgaggag tgccggacaa tcaacttccg ccgcgtcaag gacggcgcga
     3961 aggtcgtcca tgcgttcatc gagcgtcggc acccgactca cgggatcaga tagcccggtg
     4021 ccgcgcttgt cccacaggat cagacgcgtc tgccctgcca gccgttcgac gaggggcgcg
     4081 aacgggtacg ccgggtcgtc gtaaagatcg acgttgctca cccagcccgg gaccagcaca
     4141 agtggcagcg cgccgtcgcc cacgacacgg tacgcgaggt gcacgtcgcc gtttcgggcg
     4201 tagcgaactc gcccatcact cacgtcaaca tcgtgccacc accgtgatca gggttcctca
     4261 aatttcgctc atgcatcgac cgacgttaaa cagcgaacgt ggtctcaatg ccgtgagccc
     4321 cgccgtagta ctcgtcagcg acatcgggtg tggccgagcg tccgtcgaac gaccgcaacg
     4381 aaccgcagcc cggcggcggg caccccgcat tcggctagca ctttcaggcg cccgttggcc
     4441 atcgccacga tcaggtcggc ctatagggtg cgtgcttagg cgggcttcgg ctgcctcggc
     4501 gcgttcctgt tcggcgtcag ccgggtggac gggttgacag tgtgaactca gcgcgtacct
     4561 agctgcgtct catcgacggc gtgttaactc ttcagcgttg ctggcccgcc gaccgccgag
     4621 ttcaaacccg ctgcgaaatg tacctggagc gtgtcggccc gacagcgata cgatttgcca
     4681 tccggcgatc ctcgggcggt cgccgcgtcc ttcggaggtc ggcatgtcct ttgtgatcgc
     4741 ggtgccggag tatgtgacag cggccgcctc ggatttggcg aatgtcggtt cgacgatcac
     4801 tgcggccaac gccgtggcgg ccgtcccgac gtcgacggtg ctggccgccg gtggagacga
     4861 ggtgtcggcg gccgtcgcgg cgatattcgg cgcgcacgcg caggcctacc aggaactcag
     4921 tgctcaggcg gcgacgtttc accagcaatt cgtccagctt ctgaatgcgg gtgcgggctc
     4981 ctacgccaat gccgaggcgg ccaatgccac tccgctccag agcacgcagc aggatctgct
     5041 caacgcggtc aatggaccga ccgaggcgat cctcggacga ccgctgatcg gcgacggcgc
     5101 caacggcatc aacggaaccg gcgccagcgg ccagaacggc gggttgttgt ggggcaatgg
     5161 cggcgccggc gggtccggcg cagccggcca aaacggcggc aacggcggca gcggcgggtt
     5221 cttgttcggt aacggcggcc ccggtggacc cggaggggca gccattggca acggtgccgc
     5281 cggctttggc ggcgacggcg gcaacgccgt cgggcttttc ggcagcggcg gcatcggcgg
     5341 gaccgggggc aacgccggga cgcttggcgt cggcggcgac ggcggcttcg gcgggagcgg
     5401 cgggatcttg ctcggcaacg gcggcatcgg cggggcgggc gggaccggcg gcgttgccgg
     5461 ctggggcggc gacggcggcg acggcgccgg catgctctac ggcctgggcg gcgccggcgg
     5521 cgcaggcggg gccaccacga acgccgtcgg gttcgcgggc gtcggcggga acggcggcag
     5581 tgggaacatc ctcttcaacc tcatcagcac cggggccgac ggcggcgccg gcggggcggc
     5641 cgttggcggc gccaacatcg gcggccaggg cgggcagggc ggcggcggcg ccggtgagct
     5701 ctggggcatc ggtggcaacg gtggagtcgg cggggccgcc gtcaccagcg gcggcactgg
     5761 cggccccggc gggtacggcg gcgacggcgg tgcgttcttc ggaatgggcg gcgcgggcgg
     5821 cgtcggcggg gcggccatcg gcaacggcca cggcggggtt ggcgggctcg gtggcctggg
     5881 cggcctgctc ttcgggatcg gcggtgccgg cggtgacggc tacggcgcgc cgggcttggc
     5941 gttcggtggc aacggcagcc aggggggcta cggcggcctg ctcttcggca tcggagggcc
     6001 cggcggcaac ggcggcatcg gggtcacccc gggcattggc ggccccggtg gcttcggcgg
     6061 gtattacctc ttcggcccga acggaccgag cgggttgaca ccggcgtgac tcctgccttg
     6121 tggatgttgc gcccccgttg tgcaccagcg gttagccgcg atggttgctg ctgcgcgtga
     6181 atacacgggc taagctgcgg aaacggtgcg ccgacggtta ttgccgcacg agcgcgcatg
     6241 tgcggcaagt cccgatcgaa ttgtgcgtcc gcaccgacac aaaatgactt gcgctttgcc
     6301 ctaatgcctg ggggccgtcg cgtggcacca ttaggcggcc cagaccaccc aaacaagaaa
     6361 ggaagtaaca catggcagaa gagtcgagcg cggcgtttct tgaccgggtc aaggagctgc
     6421 tgacgcagaa cgaggcacgg atgaagaccg tgcaagagca gatcgcaagc cagtgggaca
     6481 cgcagcacaa ggcgatctcg gaccttgttg aagggcagtg gaatgcccag cagaaggcga
     6541 tctccgacct gatcgagcaa gggcaggacc agacggccaa gatcgcagac ctgtgggaat
     6601 ctcggcgcaa ggcgatcgcc gatcaggtcg agcagcagtg gaacgcgcag cagaagtcga
     6661 tctcggacct gatcgaaact cagcggaacg cccaaaagaa ggccatcgag gatgtgcact
     6721 ccgcgctcgg tggagtgctc ggggataagt ccgaatagcc gcacgcgcag catcgcggaa
     6781 gttaccgcgg ctcgtatgtc gtcagcgggg ctacagcatc acgcgctggt gctgtagccc
     6841 cgctgacgca aagcgtgaga tcacgatcgc cggtcggcga ccggtcccga cgaactcagg
     6901 tcgccgctat gcgcgtgggc acagatcgac cagggcatag tcgatgaggg cgtcggcctg
     6961 tcgcggcgtg agccccctag ggcccaagtc ttggacagag ctttgcagcg cgagggactg
     7021 tagctgctgc ggaggagcgc cccaggttag ctgctggcag atcttccaac cggtctgcac
     7081 cagcgccggg tcgccgccat cgaggtcctg gatgccgatg ttgtgcaagt gggtgagata
     7141 ggaagcctcg tcggcgtgcg ccgggccggc gcacacgacg ccggcgatca gtagggggac
     7201 acccaccaac gctgcgcgaa tgagcctgcc agagagcctc tctgctgcca tgactgcttc
     7261 tcctgtgtct gattgttcgc cggttcttga cggtgcacgt atatcaaagc cctaaatcac
     7321 gcgtcgcgca gcagggacgt ttcggcgctc gcgccggcga caagtttgcg gtagcgccat
     7381 ggggttcgta attcgaaatg gttacgagat ttgatcattt cggcaacggc gcggcgaatc
     7441 tttgttcgat tggacgcggg ttaccgtgtg gccgtgcgcc atcgctgcag ctgcggatcg
     7501 cgtccgccgg cttctcggcg cgatgtgctg aaatacgctg cggccgcgcc ggtcgtcctt
     7561 ggtttgggga tggccgccga cgtgctgatc ggcccggcgc gcgtggccgc ggatccctac
     7621 ttatcggcgg cggcgatggc gccgggccag ccggtcaaca tcatcagccg cgcggggtgg
     7681 ggcgcaaatg agtcgatccg gcggggagga cggctctgcg acaacggaat cagggctggg
     7741 gtcgtgcacc actccgcgac cggcaacgac tacgcgccga cagactcagc cgccatcgtg
     7801 cgctcgatct acgagtacca cacgctgaca caagggtggg gcgacatcgg ctataacgcg
     7861 ctggtcgaca agtacggcca ggtcttcgag ggcaggttcg gcggcatcac caacccggtc
     7921 gagggcgtcc acaccggcgg attcaacgcc aatacctggg gggtgtgcat gatcggcgat
     7981 ttcagccagg cgccccccac gcccgaccag ttgcgggcgg tcggccagct gcttggatgg
     8041 cggctcgcga tggacgcggt caaccccaaa ggcactgtcg cgctgacttc cgccgggggc
     8101 cccaacacgc gtttcccgcg aggtgccgcc gaaaccttgc ctagcatctt cggccatcgt
     8161 gacgtcggcg acaccgactg ccccggcaac gccggatacg cctttttgga gcggatccgc
     8221 gacatcgctg cccggttcaa gcaaccgccc agcgcacagg aattggcgga gtcgctgaag
     8281 ggcggagcga tctacgaccg ctggcaggcg acgggcggca tggccggcgg gctaggcgca
     8341 cccacgtccc ccgaagcggc ctgtggtgat tcgatccgct acgtcgtctt cgctagtggc
     8401 gcgatctact ggtcgccggc caccggccct cagccgctca ccggcaggat ctacgaggcc
     8461 tgggcgacgc tgcgctacga gcgcggcccg ctgggcctgc cgaccagcgc cgagatccac
     8521 gagccagagt ggatcgtgca gaacttccag cacggcaccc tgaacttcaa ccggcaaacc
     8581 tcgaccgtca tgtcggtaat agacggggta gccactgtgg ttccgccgcc gcccgcgggc
     8641 ggaccgcctg tgcagcttga acggttctcc gcagcacgca atcccgtcgt ctagtcagct
     8701 ctgggatttc accctggaga taccagcctg gctggacgct actgacgggg cgatccctgc
     8761 cgtccggcta cgacaacccg ttcgtcccgt tcaggccgag cagttgcccg ccggtgccgc
     8821 cggtgccggc ggtgccctcg gcggtgccca tccccgggtt accgccgtta cc
//
LOCUS       DASSGQ010000160         4447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1014217,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000160 DASSGQ010000000
VERSION     DASSGQ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014217"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VFI55_07185"
     CDS             complement(<1..659)
                     /locus_tag="VFI55_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HET7073849.1"
                     /translation="MSTTGTGRPLRVIQWTTGNIGRRSLHAIIGRPDMELVGVYAHGA
                     DKVGVDAAELAGWPDSTGVQATNDIDALLALRADACCYNPLWPNVDELVRLLESGVNV
                     CSSAAWITGGKQTPEDRKLIADACERGGSTIFGSGAHPGMTNMVGMVLSASCERVDEI
                     RITESVDCSTYESAETQTAMGFSQDPDTPGLADNVRRESEVFAESAAMMANAIGAKLD
                     R"
     gene            complement(695..1117)
                     /locus_tag="VFI55_07190"
     CDS             complement(695..1117)
                     /locus_tag="VFI55_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET7073850.1"
                     /translation="MAELSDKVIEFLSTGTRTGMLGYIAADGRPLVAPVWFVVDDGQL
                     VFNTGRDTSKGRALKRDSRVVICVDDPHPPYSFVQVQGVATMTDDPQDVLHITTRTGA
                     RYMGADRADEFGRRNAVPGELVVRVRPTKVIAGFDISD"
     gene            complement(1121..2236)
                     /locus_tag="VFI55_07195"
     CDS             complement(1121..2236)
                     /locus_tag="VFI55_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7073851.1"
                     /translation="MDFKLTDEQVLLRDTTRDLLSRAYDPESRNKVIETDLGWSRDVW
                     SRLADTGILSLGFEPDEAGQIEIMVVLNEIGKRLAPEPVVHAALVPGALIAESGSDAQ
                     RQLLDEVASGQRLLAFAHVETGHRKPTAAVATRAVRQGDSWTLSGTKNPVLAGDCADT
                     LVVSAALPDGGTGLFLVEGEAVTRYPYPTFDGQRGAQIQLDSAPAEPLGTASDAHDAS
                     PVIRAAIIRIQSALCAEAVGAMEEALRLTTDYLKTRKQFGVTLNKFQALTQRAADMYV
                     SLELARSMSLYAAMSIADGNLDPVIASRAKLQIGRSGRHIAEESIQLHGGIGMTAEYP
                     VGHYAARLTAIEQTLGSSQDHLQILIDHIAEYDMARL"
     gene            complement(2244..3401)
                     /locus_tag="VFI55_07200"
     CDS             complement(2244..3401)
                     /locus_tag="VFI55_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7073852.1"
                     /translation="MQLALTPEEAAFRDELRAFYTTEIPEELREQVRHGLPVTKEQIV
                     TSHKILNDHGLAVPNWPVEWGGKDWTPTQYQIWTDEMQLACVPEPLNFNVKMVGPVIA
                     EFGSQEIKQRFLPPTASIDIWWCQGFSEPEAGSDLASLRTTAVRDGDSYVVNGQKTWT
                     TLGQYADWIFCLVRTDPQAPKRQAGISFLLMDMATPGITLRPIKTIDGGREVNEVFFS
                     DVRVPADQLVGQENQGWTYAKFLLGNERTGIAGVGRTKVRLAEVKKHAAETRLLDDPL
                     FAARLAEAENELLALELTQARVVTDSADGKPNPASSVLKMRGSQLQQVATELLVEVAG
                     PDALPVDGPNIASPAWAQSSAPRYLNYRKTSIYGGSNEVQRNIIASTILGL"
     gene            3589..>4447
                     /locus_tag="VFI55_07205"
     CDS             3589..>4447
                     /locus_tag="VFI55_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HET7073853.1"
                     /translation="MRTELPAERLHRRLAAEPDSHADSDSAEFDSDQGETDQDQNSLL
                     PRWLPDAAQSRGWMARMRADPGRAGAIALAIVAALAVLVTVFALVRDRSAPVMSAKLP
                     PVEKASTASPRSSASPTAGEPAGPDRPVVVSVVGLVHTPGLVTLAPGARIADALQAAG
                     GPVNGADTIGLNMARPVGDGEQIVVGLAPVSGQPTALGSSVASGSAPASGAPGSGAPG
                     PASRTVKPKAGEVLNINTATVEQLDALPGVGPVTAAAIVAWRQANGKYTSVDQLADVD
                     GIGPARLDKL"
BASE COUNT          671 a         1493 c         1488 g          795 t
ORIGIN      
        1 atcctgtcca gcttggctcc gatcgcgttg gccatcatcg cggccgattc cgcgaagact
       61 tcgctttccc gccgcacgtt gtcggccagc cctggcgtgt cgggatcttg tgagaacccc
      121 atcgccgttt gggtttccgc cgattcgtac gtcgagcagt ccaccgactc ggtgatccgg
      181 atctcgtcga cgcgctcgca ggaggcgctg agcaccatgc cgaccatgtt cgtcataccg
      241 ggatgggccc cgctgccgaa gatcgtcgaa ccgccccgtt cacacgcgtc cgcgatgagc
      301 ttgcggtcct cgggcgtctg cttgccgccg gtgatccagg ccgcgctgga acacacgttg
      361 acgcccgatt ccagcagccg cacgagttca tcgacgttgg gccacaacgg gttgtaacaa
      421 cacgcgtcgg ccctcagggc cagcagcgca tcgatgtcgt tggtggcttg caccccggtg
      481 gaatccggcc agccggccag ttcggcggcg tcgacaccca ccttgtcggc cccgtgcgcg
      541 tacaccccga ccaattccat gtcgggtctg ccgatgatgg cgtgcagcga acgccgtcct
      601 atgttcccgg tcgtccactg gatcacccgc aggggacggc cggtgccggt tgtactcatc
      661 ggggctcctc tgacgaaggg cgcgggttgg accatcagtc gctgatgtcg aatccggcga
      721 tgaccttggt cgggcgcacg cgcaccacca gttcgccggg aacggcgttg cggcggccga
      781 attcgtcggc gcggtcagcg cccatgtagc gcgcgccggt cctggtggtg atgtgcagca
      841 catcctgcgg atcgtcggtc atcgtggcca caccctgcac ctgcacgaac gaatacggcg
      901 ggtgggggtc gtccacgcat atcaccactc gcgaatcacg tttcagagca cggcctttcg
      961 acgtgtctcg gccggtgttg aacaccagct gaccgtcgtc gaccacgaac cacaccggcg
     1021 ccaccagcgg ccggccgtcg gcggcaatat agccgagcat tccggtgcgg gtgcctgtcg
     1081 acaggaactc gatgactttg tcagagagct cggccatgtg ttacagcctg gccatgtcgt
     1141 attcggcgat gtgatcgatc aggatctgca agtggtcctg cgaggagccc aacgtctgct
     1201 cgatcgcggt gagccgggcg gcgtagtggc cgaccgggta ctccgcagtc atgccgatgc
     1261 cgccgtgcag ctggatcgac tcctcggcga tatgccggcc cgaccgaccg atctgcagct
     1321 tggcccgcga cgcgatgacc gggtcgaggt tgccgtcggc gatcgacatc gcggcgtaga
     1381 ggctcatgct gcgggccagc tccagcgaca cgtacatgtc ggcggcccgt tgggtgagcg
     1441 cctggaattt gttgagcgtg acgccgaatt gcttgcgggt cttcaggtag tcggtggtca
     1501 gtcgcagcgc ttcctccatc gcgccgacgg cttcggcgca caacgccgac tggatgcgga
     1561 tgatggcggc gcggatgacg ggggaggcgt cgtgggcatc cgacgcggtc ccgagaggct
     1621 cggcgggagc ggagtccagt tggatctgcg cgccgcgctg accgtcgaac gtcggatacg
     1681 ggtacctggt cacggcctca ccctcgacca ggaacaatcc ggtgccgccg tccggcaacg
     1741 cggcgctgac caccagggtg tccgcgcagt caccggcaag caccgggttc ttcgtcccgc
     1801 tgagcgtcca cgaatctccc tgccgcacag cccgagtggc tacggccgcg gtcggtttcc
     1861 ggtggcccgt ttcgacgtgc gcgaacgcga gcaaccgttg accggacgcg acctcatcca
     1921 gcagttgccg ttgcgcgtcg ctcccactct cggcgatcag cgcaccgggc accagggcgg
     1981 cgtgcacgac gggctcgggc gccaaccgct tcccgatctc gttgagtacc accatgatct
     2041 cgatctggcc ggcctcgtcg ggctcgaatc cgaggctcag gatcccggtg tcggcgagcc
     2101 ggctccagac gtctcgactc cagccgagat cggtctcgat gaccttgttg cggctctccg
     2161 ggtcataggc gcgcgacagc aggtcgcggg tggtgtcgcg gagcagaacc tgttcgtcgg
     2221 tcaacttgaa gtccatggcc gcctcacaat ccgagaatgg tcgacgcgat gatgttgcgc
     2281 tgcacttcgt tgctgccgcc gtagatggat gtcttgcggt agttcaggta gcgcggggcg
     2341 ctggactgcg cccaggccgg tgaagcaatg ttcgggccgt cgaccggcag cgcgtcggga
     2401 ccggccacct cgacgagcaa ctcggtggcc acctgctgca gttgactgcc gcgcatcttg
     2461 agcaccgacg acgccgggtt gggcttgccg tccgcggagt ccgtcaccac ccgggcctgc
     2521 gtgagttcca gtgccagcag ctcgttttcg gcctcggcca gacgcgccgc gaacagcgga
     2581 tcgtccagca atctggtttc cgcggcgtgc ttcttcacct cggcgaggcg caccttggtc
     2641 ctacctaccc cggcgatgcc ggtgcgctcg ttgcccaaca ggaactttgc gtacgtccag
     2701 ccctgattct cctgtcccac aagctgatcg gccggtaccc gaacgtcgga gaaaaacacc
     2761 tcgttgactt cgcggccgcc gtcgatcgtc ttgatcggcc gcagggtaat tcccggcgtc
     2821 gccatgtcca tgagcaagaa cgagattccg gcctggcgct tgggcgcctg cgggtcggtg
     2881 cgcaccaggc agaagatcca gtccgcatac tggcccagcg tcgtccacgt cttctgcccg
     2941 ttgacgacgt agctgtcgcc gtcccgcacc gcggtggtgc gcaaggaggc gaggtcggag
     3001 ccggcctccg gctcggagaa cccctggcac caccagatgt cgatgctggc cgtcggcggc
     3061 aggaagcgct gcttgatctc ctgggacccg aattcggcga tcaccggccc gaccatcttg
     3121 acgttgaaat tcagcggctc cgggacgcac gccaattgca tttcgtcggt ccaaatctgg
     3181 tattgggtcg gcgtccagtc tttgccgccc cattcgaccg gccagttcgg caccgcgagc
     3241 ccgtgatcgt tcaggatctt gtgactggtg acgatctgtt cttttgtcac cggcaatccg
     3301 tgccgcacct gctcgcggag ctcttcgggg atctcggtgg tgtagaaggc gcgaagctcg
     3361 tcgcggaacg cggcttcctc gggtgtgagc gccagctgca tgacatcctc ccgtgctctg
     3421 agggcgggcg tggcgacctg ccgcgaatgg ggtccggtcg tttgctccgg cttccatagt
     3481 gacccatccc gtgaagggct tgtgcacagc ctcggcttaa tccacaggcg ggcatcgcga
     3541 cgccccgccc ggcccgattg tggcggtcgg cgcacttacg gtcggcgcat gcgaactgaa
     3601 cttcccgccg agcgactaca tcgacgactc gctgccgagc cggattcgca cgccgactcc
     3661 gacagcgcag agttcgactc agaccagggc gagacggatc aggaccagaa ctcgctgttg
     3721 ccgcggtggc ttcccgatgc ggcccaaagt cgggggtgga tggcccggat gcgcgccgac
     3781 ccgggccgtg ccggcgcgat cgcgttggcg atcgtggctg ccctcgcggt gctggtcacg
     3841 gtattcgcct tggtgcgtga ccggtccgcg ccggtgatgt ctgccaagct cccgccggtg
     3901 gagaaggcgt ccacggcgag ccccaggtcg tcggcgagcc cgaccgccgg cgagccggcc
     3961 ggtcccgacc gaccggtggt ggtcagcgtg gttggcctgg tgcacactcc cggactggtc
     4021 accctggcgc ccggcgcgcg catcgccgac gcgctgcagg cggccggtgg cccggtgaac
     4081 ggcgccgaca ccatcggatt gaacatggcc cggccggtcg gcgacggcga gcagatcgtg
     4141 gtcgggcttg ctcccgtctc ggggcagccg acggcgctgg gcagttcggt ggcctccggt
     4201 tcggcaccgg catccggggc accgggatcc ggagcacctg ggcccgcttc acggacggtg
     4261 aagccgaagg ccggtgaggt gctcaacatc aacaccgcaa ccgtggagca gctggacgcc
     4321 ctgcccgggg tcggaccggt caccgccgcc gcgatcgtgg cgtggcggca ggctaacggc
     4381 aaatacacca gcgtcgacca gctcgccgat gtcgacggca tcggtccggc gcggctggac
     4441 aaactgc
//
LOCUS       DASSGQ010000161         4708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_498312,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000161 DASSGQ010000000
VERSION     DASSGQ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498312"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="VFI55_07210"
     CDS             complement(<1..1134)
                     /locus_tag="VFI55_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PPE domain-containing protein"
                     /protein_id="HET7073854.1"
                     /translation="MTYLITEPQVLAAAAADVEQIGSAISAANAAAAGPTAALVAPAG
                     DEVSAAITQLFGAYSQDYQAVVAKAAVFHGQFTQALSAAGGAYAAAEAAALQALGAFE
                     ASPMASTSANPAATVAIVIGGSGNPIPGQPYIDGVLRWATQGGFTWNSVQALFTPEGL
                     YPLTGTKSLPLDMSVSQGVQILDGAIKQQIAAGNSVLVQGYSQSSIIASLEMQNLLAS
                     PNPPTGTQLSFNLLGDPMNPNGGLLARFAGLTFPSVGLDFYGATPANTPWTTNIYSLE
                     YDGFADFPRYPIDIFADLNAVAGIAFVHPTYTHIDPATLPSGDIVKLPVSPSYTGPLA
                     NTTYWMVLTPDLPLLDPLRAIPVVGNPLADLLQPDLTYLVNWGY"
     gene            complement(1348..2184)
                     /locus_tag="VFI55_07215"
     CDS             complement(1348..2184)
                     /locus_tag="VFI55_07215"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HET7073855.1"
                     /translation="MAEATLRNLPGAWNFRDVADSATALRPGLLFRSSELSRLDDDGR
                     ATLRRLGITDVADLRASREVARRGPGLVPDGVEIHLLPFPDLADQEDPDDAAPHEHAF
                     QRMLTGESDQSDRSDESINVAAARFMTDEYRQFPTRNGAQHALRSVITLLGAGRPVLT
                     HCFAGKDRTGFVIATVLEVLGVDRDTIVADFLRSNAAVPELRARIQEMIQQRPDTELT
                     PEVETFTEARLSDGVLGVRAEYLAAARQTIDDEFGSLAGYLSAAGITAADLDRLRAAL
                     LA"
     gene            complement(2177..3397)
                     /locus_tag="VFI55_07220"
     CDS             complement(2177..3397)
                     /locus_tag="VFI55_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7073856.1"
                     /translation="MSAKASDYHKRLSDFMTDYVFPAENDYDKYRHDAGPNDHTVPPI
                     IEELKTKAKAAGLWNLFLPAESGLTNLDYAPLAELTGWSLEIAPEALNCAAPDTGNME
                     TLHLFASNEQRKQWLEPLLNGEIRSAFSMTEPAVASSDARNIATSIVRDGADYVINGR
                     KWWTSGAADPRCKILIVMGRTNPDAASHQQQSMVLVPIDTPGVTVVRSTTVFGYQDQP
                     GHCEIVYDNVRVPATNLLGQEGGGFAIAQARLGPGRIHHCMRALGGAERALALMVDRA
                     RNRIAFGRPLADQGLVQQSIAKSRNEIDQARLLCEKAAWTIDRHGNRAAHLLVSQIKA
                     VAPQVACDTIDRAIQVHGAAGVSEDTALARLYAWHRAMRIFDGPDEVHMRTIARTEIG
                     REQSAFAAAVTPHG"
     gene            3563..4024
                     /locus_tag="VFI55_07225"
     CDS             3563..4024
                     /locus_tag="VFI55_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7073857.1"
                     /translation="MTESRPPFPPFTYETAIQKVQAAEDAWNTRDPEHVSLAYTPDSQ
                     WRNRDEYVVGRAEIVAFLTRKWERELDYALRKSLWDFHDNRIAVRFQYECRDHTGQWY
                     RSYGNELWEFSPSGLMARREASINDVAIDESERRYFGPRPSSEHGRDIPLW"
     gene            4332..>4708
                     /locus_tag="VFI55_07230"
     CDS             4332..>4708
                     /locus_tag="VFI55_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HET7073858.1"
                     /translation="MTDAKTVVGVVAESGADERRVALVPKAVASLVNTGLSVVVESGA
                     GVGALLPDELYTAAGASIGDAWAADVVVKVAPPTLDEVGRLRRGQTLIGFLAPRNAEN
                     SIGALRAAGVQGFAVEAIPRISRA"
BASE COUNT          774 a         1449 c         1668 g          817 t
ORIGIN      
        1 gtaaccccag ttgaccagat aagtcaagtc cggctgcagc aggtccgcca gcggattgcc
       61 caccacgggt atggcacgca gcggatccag cagcggcaga tccggggtga gcaccatcca
      121 gtaggtggta ttggcaagcg ggccggtgta cgacggtgac acgggcaatt tgacgatgtc
      181 gccggatggc agcgtcgctg gatcgatatg cgtataggtg ggatgcacga aggcgatgcc
      241 cgcaacggcg ttgaggtccg cgaagatatc gatcgggtac cgcgggaagt cggcgaaacc
      301 gtcgtattcg agcgagtaga tatttgtagt ccacggcgtg ttggccggcg ttgcgccgta
      361 gaagtccaga cccacactcg ggaacgtcag gccggcgaac cgcgccagga gtccaccgtt
      421 ggggttcatc ggatcgccca gcaggttgaa ggagagttgc gtccccgtcg gcgggttcgg
      481 gctggccaac agattctgca tctccagcga cgcgatgatg gagctctgtg agtagccctg
      541 cacgagaacg ctattgccgg cggcgatttg ctgtttgatc gcgccgtcga gaatctgcac
      601 gccctggctc accgacatgt ccaggggcag ggatttggtc ccggtgagcg ggtacaggcc
      661 ttccggagtg aaaagggctt gcacggagtt ccaggtaaac ccaccttggg tggcccacct
      721 gaggaccccg tcgatgtaag gctggcccgg tatcgggttt ccgctgccgc cgatgacgat
      781 ggcaacggtc gcggcggggt tcgccgacgt ggacgccatc ggcgacgcct cgaatgcgcc
      841 aagcgcttgc aacgccgcag cctcggctgc cgcgtaggca cctccggcgg cggacaacgc
      901 ctgggtgaat tgcccgtgga atacagcggc tttcgcgacc accgcctggt agtcctggct
      961 gtatgcgccg aatagctgcg tgatggccgc cgacacctcg tcgccggcgg gggccaccag
     1021 ggcggccgtc ggccccgccg ccgccgcgtt ggccgcgctg atcgccgaac caatctgctc
     1081 gacgtccgcg gcggccgccg ctaagacctg cggctcggta atcagatatg tcatcggtcc
     1141 cccttcgcga cgacggaact tctctcggcc aaaccgaagg cccggcgagc ccgggccacc
     1201 gagaagtcag ccgtagtgag cgtagagcca tatcgcaagc ctgtgcggga tttctcacca
     1261 agcgatgtca gcagtgcggt cggcattggt ggaggggcaa ccagtcgttc gcctctactg
     1321 cgtgaaaagg gctctacggc agtcggttca ggcgagtagc gcggcccgca gccgatcgag
     1381 gtcggccgcg gtgataccgg cggcgctcag atagccggcc agcgacccga actcgtcgtc
     1441 aatcgtttgg cgcgcggcgg ccaggtactc cgcgcgcacg ccgaggacac cgtcggacag
     1501 ccgggcttcg gtaaaggtct ccacttccgg agtcagttcg gtgtccggac gctgctggat
     1561 catctcctgg atccgggccc gaagctccgg tacggcggca ttgctgcgca agaagtcggc
     1621 gacgatggtg tcgcgatcga cgccgagcac ctcaagcacg gtcgctatga cgaatccggt
     1681 gcgatccttg cccgcgaagc agtgggtgag caccggacgc ccggcgccca acagcgtgat
     1741 gacggatcgc agcgcgtgct gcgctccgtt acgtgttggg aattgccggt attcgtcggt
     1801 catgaagcgt gcggcggcca cgttgatgga ctcgtcggac cggtcggact gatcggattc
     1861 gcccgtcagc atccgctgaa acgcgtgttc gtgcggcgcc gcgtcgtccg ggtcttcctg
     1921 gtcggccaga tcggggaacg gcagcaggtg gatctcgacg ccgtcgggaa ccagccccgg
     1981 acctcgccgg gcaacctccc gggatgcgcg caggtcggcc acgtcggtga tccccagccg
     2041 gcgcagggtg gcccggccgt cgtcgtcgag gcggctcagc tcgctggacc ggaacagcag
     2101 cccgggccgc agggcggttg cactgtcggc gacgtcacgg aagttccagg cgcccggcag
     2161 attccggagg gtggcctcag ccatgcgggg tgaccgccgc ggcgaacgcg gactgctcac
     2221 ggccaatctc ggtgcgcgcg atcgtccgca tgtgcacctc gtcggggccg tcgaagatcc
     2281 gcatcgcgcg gtgccaggcg tacaggcggg ccagcgcggt gtcctcgctg acgcctgccg
     2341 cgccatgcac ctggatggcg cggtcgatgg tgtcacaggc cacctgcggc gccaccgcct
     2401 tgatctgcga cacgagcaga tgcgcggccc tgttgccatg ccggtcgatt gtccaggccg
     2461 ccttctcgca gagcagcctg gcttggtcga tttcgttgcg ggacttggca attgactgct
     2521 ggaccaggcc ttggtcggcc agcggacggc cgaatgcgat ccggttgcgt gcccggtcca
     2581 ccatgagggc caacgcacgt tcggctccgc cgagtgcccg catgcagtgg tggatgcgac
     2641 ccgggccgag ccgcgcctgc gcgatcgcga agccgccgcc ttcctggccg agcaggttgg
     2701 tggccggcac ccggacgttg tcgtacacga tctcgcagtg cccgggctgg tcttggtagc
     2761 cgaaaaccgt cgtcgagcgg acaaccgtca cacccggtgt gtcgatgggc accagaacca
     2821 tcgactgctg ctggtggctg gccgcgtcgg ggttggtgcg acccatcacg atgaggatct
     2881 tgcagcgcgg gtccgccgcc ccggacgtcc accacttgcg gccgttgatc acatagtcgg
     2941 cgccgtcgcg cacgatagag gtcgcgatgt tgcgggcgtc gctgctggcg accgccggct
     3001 cggtcatcga gaaggcgctg cggatctcgc cgttcagcaa cggctccagc cactgcttgc
     3061 gctgctcgtt gctggcgaac aggtgcaggg tctccatgtt cccggtgtcg ggggccgcgc
     3121 agttgagcgc ctcgggtgcg atttcgaggc tccagccggt cagctcggcc agcggggcgt
     3181 agtccaagtt ggtcaggccc gactcggccg gcaggaacag gttccacagg ccggccgcct
     3241 tggccttggt tttcagttcc tcgataatcg gcggaacggt gtggtcattc gggccggcgt
     3301 cgtggcggta tttgtcgtaa tcgttctcgg ctggaaagac gtagtccgtc atgaagtcgg
     3361 acagccgctt gtggtagtcg ctcgctttgg ccgacatcgc gaagtccata tccgccacga
     3421 taggacacgc gtcatatgcc tccgcgagca gacgcagaat cgcgtgaatc cgcgtgtatc
     3481 ggtgcgattc tgcgttcccc cgcacgcggg aggtaccccc agctcgcggg ggttgagggc
     3541 ggcggcgcgc aggatggggt gcatgaccga gagccgcccg ccatttcctc cattcaccta
     3601 cgaaacggcg atccaaaaag tccaggccgc cgaagatgcc tggaacaccc gcgatcccga
     3661 acacgtcagc ctggcttata cgcccgattc acaatggcga aaccgcgacg agtacgtcgt
     3721 cggtcgggcc gagatcgtgg cgtttctgac ccgcaaatgg gagcgcgaac tcgactatgc
     3781 gctacgcaag agcttgtggg actttcacga caaccgcatc gccgtgcggt ttcagtacga
     3841 gtgccgcgat cacaccggcc agtggtaccg cagctacggc aacgaactgt gggaattcag
     3901 cccgtcgggg ttgatggcgc gccgggaagc cagcatcaac gacgtggcga tcgacgagtc
     3961 cgagcgccgc tatttcgggc cccgcccgtc ctctgaacac ggccgagata tcccgctctg
     4021 gtagcggctg gctgacgtgg gagtacctcg cagccgcgat tcggcgaggg ggagagccgg
     4081 gcagtcaggc tggccgctca ccaaaattgg tgactcaacg ccggcgaagg gcatgccatg
     4141 gtggtcgtgc gcggtgctga actgggccag cgcaccggga gtcgaggtcg atgtcgggac
     4201 ccgctcgcag ggcagagctt ggcaatcagg ttagggtgtc tgagcgcgcc cggccacgat
     4261 acccgccggg cactccgatt ggaactagta gcagtgcctc aacttaagga aagaaagtag
     4321 atgtacgcgt catgacagat gcgaagacgg tggtgggggt ggtggccgag tccggggccg
     4381 acgagcgccg ggtggcgctg gtgccgaagg cggtcgcgtc gctggtcaac accggcctgt
     4441 cggttgtggt cgagtccggt gcgggtgtgg gggcgctact gcccgacgag ctctacacgg
     4501 cggccggcgc cagtattggg gatgcgtggg cggccgatgt ggtggtgaag gtggcgccgc
     4561 cgaccctaga tgaggtgggg aggttgcggc gggggcagac gttgatcggt tttttggcgc
     4621 cgcgtaatgc ggagaattcg atcggtgcgc ttagggcggc gggggtgcag gggtttgcgg
     4681 tggaggcgat tccgcggatt tcgcgggc
//
LOCUS       DASSGQ010000162         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_133378,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000162 DASSGQ010000000
VERSION     DASSGQ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133378"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VFI55_07235"
     CDS             <1..984
                     /locus_tag="VFI55_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioester reductase domain-containing protein"
                     /protein_id="HET7073859.1"
                     /translation="AGDKGEANLGLDQQTWQRLADTVDLIVDPAALVNHVLPYSELFG
                     PNAVGTAELLRIALTSKQKPYTYVSTIGVGDQIEPSKFVEDADIRKISATRAINDSYA
                     NGYGNSKWAGEVLLREAHDLCGLPVAVFRCDMILADTTYAGQLNLPDMFTRLMLSLAA
                     TGIAPGSFYELDDEGNRQRAHYDGLPVEFIAAAIATLGAQNVQSFQTYHVMNPYDDGI
                     GLDQYVDWLIDPDNGADCPIQRIDDYGEWLQRFEGAMRALPERQRQYSLLPLLHNYQK
                     PEKPLRGSLAPTDRFRAAVQEAKVGPDKDIPHVSAPIIVKYITDLQLLGLL"
     gene            1163..>2034
                     /locus_tag="VFI55_07240"
     CDS             1163..>2034
                     /locus_tag="VFI55_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7073860.1"
                     /translation="MSTREERLARRIDDLYTNDEQFAAAKPSNEIARALAQPGMGLAQ
                     VIKTVMTGYADRPALGQRAVRFVTDASGRTGMELLPRFETVTYGELAERVNAFASALQ
                     ADVRPGDRVCVLGFTSVDYTTVDIALAQLGAVSVPLQTSAAIAQLLPIVTETEPTLIA
                     SSVDSLPDAVELILSGHAPGRLVVFDDRPEVDDHRDAVAAARARLSDCAVTVETLADV
                     IARGNASGHAVSGDAPGGSVDDLSLLIYTSGSTGAPKGAMNTERRAIKFWRKSDAAFE
                     STDPSITLNFMPMS"
BASE COUNT          369 a          712 c          649 g          304 t
ORIGIN      
        1 gccggcgaca agggcgaggc caacctcggc ctggaccagc agacctggca acggctggcc
       61 gacaccgtcg acctgatcgt cgaccccgcc gccctggtca accacgtcct gccctacagc
      121 gagctgttcg gccccaacgc cgtgggcacc gccgaactgc tgcggatcgc actgaccagc
      181 aagcagaagc cgtacaccta cgtgtccacg atcggggtgg gcgaccagat cgagccgtcg
      241 aagttcgtcg aggacgccga catccggaag atcagcgcga cgcgggcgat caacgactcc
      301 tacgccaacg gctacggcaa cagcaagtgg gcgggcgagg tgctgctgcg cgaggcccac
      361 gacctgtgcg gcctgccggt cgcggtgttc cgctgcgaca tgatcctggc cgacaccaca
      421 tatgcgggac agctcaacct gccggacatg ttcacccggc tgatgctcag cctggccgcc
      481 accggcatcg cgccgggttc gttctacgag ctggacgacg aaggcaaccg gcaacgcgcc
      541 cactacgacg ggctgcccgt cgagttcatc gccgcggcga tagccaccct gggggcgcag
      601 aacgtgcaaa gcttccagac ctaccacgtg atgaacccgt acgacgacgg catcggcctc
      661 gaccagtacg tcgactggct gatcgaccca gataacgggg ctgactgccc gatccagcgg
      721 atcgacgact acggcgaatg gttgcagcgg ttcgaagggg cgatgcgggc cctgccggaa
      781 cggcagcgcc agtactcgct gctgccgctg ttgcacaact accagaagcc ggagaagccg
      841 ctccgcggat cgctggcccc gaccgaccgg ttccgtgcgg cggtgcagga ggcgaaagtc
      901 ggcccggaca aagacattcc gcatgtctcg gcgccgatca tcgtcaagta catcaccgac
      961 ctgcaattgc tcggattgct ctgacactgc caccctcgat cgccggcccg cgcgggccgg
     1021 cgatcttctt tgcgtcgtgg gcgtgggcga tctcacacct gcggccgtgc gcggaccatt
     1081 gagtaatatc gttactttag ctaacgtttt aggtgttaaa gcgcagctca cgtcacactc
     1141 agggtcataa aaggggactg ttatgtcgac tcgggaagag cggctcgccc gccgcatcga
     1201 cgatctctac accaacgacg agcagttcgc cgccgccaaa cccagcaacg aaatcgccag
     1261 ggcgctggcg cagccgggca tggggctggc gcaggtcatc aagaccgtca tgaccggtta
     1321 cgccgaccgg ccggcgctgg gccagcgcgc ggtgcgattc gtcaccgacg cgagcggtcg
     1381 caccggcatg gagttgctgc cgcggttcga gaccgtcacg tatggcgagc tggccgaacg
     1441 cgtcaacgcg ttcgcgagcg cgttgcaggc cgacgtccgg cccggcgacc gggtctgcgt
     1501 gctcgggttc accagcgtcg actacaccac cgtcgacatc gcgctggccc agctgggcgc
     1561 ggtgtccgtt ccattgcaga ccagcgcggc aatcgcgcaa ctgctgccca tcgtcaccga
     1621 gaccgagccc acgctgatcg ccagcagcgt cgacagcctg cccgacgccg tcgaattgat
     1681 cctgtcgggc cacgcgcccg gccggctggt ggtgttcgac gaccgccccg aggtcgacga
     1741 ccaccgcgac gccgtggcgg ccgcccgggc tcgcctttcc gactgcgcgg tgacggtcga
     1801 gacgctggcc gacgtgatcg cgcgcggcaa cgccagcgga cacgccgttt cgggtgacgc
     1861 acctggcggc tccgtggacg acctttcgct actgatctac acctccggca gcacgggcgc
     1921 gcccaagggc gcgatgaaca ccgagcgccg cgcgatcaaa ttctggcgca agtccgacgc
     1981 cgccttcgag agcaccgacc cgtcgatcac gctcaacttc atgccgatga gcca
//
LOCUS       DASSGQ010000163        28165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1014406,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000163 DASSGQ010000000
VERSION     DASSGQ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 28165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28165
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014406"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFI55_07245"
     CDS             <1..430
                     /locus_tag="VFI55_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET7073861.1"
                     /translation="LTPNGPVDAKIIYDNYSLGLGVVRFPSELVATVQVTGQTPVDED
                     YTDYFYTQASIREPGDTGDVPSGRAARFLALQQEVIKQDFFTWENMKYLEKPNLAPEE
                     AHDYAALRRWAHRFYPGTQPSPSDFGYTPGGEPDPAASKV"
     gene            443..2041
                     /locus_tag="VFI55_07250"
     CDS             443..2041
                     /locus_tag="VFI55_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7073862.1"
                     /translation="MCPDRSLPLGPTDFGVDRFTVPAVLDRRAAQHPDLVMMSIAGVD
                     VTFEQMRRRSAAAATMLADLGVGRGDCVALFTATCPEWVYFWLGAARIGAVSAAVNAA
                     NKGEFLLHTLRLARAKVIVTDADRRPRVDEVADALETLTDIVMQDDSLTSALNLGGDC
                     PPADGQGGAADVGALFYTSGTTGPSKAVATTWHYLFTVAATVASAWEFRPGEVLWTAM
                     PLFHLSAAPSVLAPMLVGATTVLAKAFHPTQVWDDIRTCGAIGFAGAGAMVSMLQNLP
                     ADPRDARLPLRFISAAPIDASSYRDIEKRYDCRIVTMYGMTEAFPIAVKGVADEGVPG
                     TSGRPNPNFEVRIVDERGDPLPAGTVGEIACRPRYPHVMSEGYISQGPRVDPHQDWFR
                     TGDLGRLDTDERGYQNLTYVDRIKDSLRRRGENVSSVEVESVVMRHPAVAEAAAVGVP
                     SELGEDDILLVVILRPGATLDCAELLDFCAARMPYFWVPRFVEAVDELPKNGIGRIRK
                     DLLRARGVSAAAWDRENRGHAVSR"
     gene            2135..2560
                     /locus_tag="VFI55_07255"
     CDS             2135..2560
                     /locus_tag="VFI55_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7073863.1"
                     /translation="MTITYQEEQLLQAATGRAAILDLCARHNRAYSDGDRERWIATFR
                     HSGARYIRDGEVFEDLRAAFDGGDGQRLVTLDHEIRIDGVHASHRCVVVLFAAMYGDT
                     TLRATGTYRDALVYERGGWYFTSRALEWDSVPSRHPLVM"
     gene            2574..3116
                     /locus_tag="VFI55_07260"
     CDS             2574..3116
                     /locus_tag="VFI55_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HET7073864.1"
                     /translation="MDRDLSYQVVFGSYEDALRMVGAKSEPRTARTPVSGARIQLFAA
                     MVHDGNLSYWDAEFARRKWGGLLAPPAALMGWLIPPPWEPSRPGRARRPAASVVLRVP
                     LPGTTFINAANDVEFLQPIVEGDLLTVVEELVAVSPEKRTRLGIGHFVETLETYRRQD
                     GAVVATCRNTLFRFTPAAAP"
     gene            3113..3559
                     /locus_tag="VFI55_07265"
     CDS             3113..3559
                     /locus_tag="VFI55_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020786650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET7073865.1"
                     /translation="MTGDGLHWDAIAVPVDLPEVVDEISYQRVVENAGATWDYFPGHF
                     DPGYARTQGNPTIYLNTMHLAGFADRVATDWAGPGSRVVRRSMRLAVPVYAGDTMVGR
                     GRAVTKRRDTSVDPPRCLVDIEIQVTNQHGTLCCPVELTLQVPESF"
     gene            complement(3573..5045)
                     /locus_tag="VFI55_07270"
     CDS             complement(3573..5045)
                     /locus_tag="VFI55_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HET7073866.1"
                     /translation="MTDNPGHRWYDELFIGGNWRKPANPQQLAVISPHSEEPIGHAPA
                     AGPEDVDAAVAAARRAFDHGPWPRMTPGERIGKLEELAAIYGRHVDEMSDLITAEMGS
                     PRSFSRLGQAAGAASMIHLSLTVARDFGWVERRRGVLGEAQLRRAPVGVVGAIVPWNV
                     PQCLIMPKLIPALLAGCAVVIKPAPETPLDALWLAEMIEQVGLPEGVVSVLPGGPDAG
                     EALVRHPGVDKIAFTGSSATGRRIAVLCGEQLKRVSLELGGKSAAIVLDDADVGKTVA
                     GLKTAGLMNNGQACVAQTRILVGERRHDEFVDALAEMMSALRVGDPADDATDIGPLVA
                     QRQQRRVQDYIRSGRREGASVIVGGEDSPAERGWYVRPTLFADASNDMRIAREEIFGP
                     VLTVLRYRDEDDAIRIANDSDYGLAGSVWTADAARGLRIAAEVRTGTYGINMYMLDIS
                     TPFGGFKQSGLGREFGPEGLDEYVELQSVVCSGKMPPLPG"
     gene            5160..6518
                     /locus_tag="VFI55_07275"
     CDS             5160..6518
                     /locus_tag="VFI55_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secreted hydrolase"
                     /protein_id="HET7073867.1"
                     /translation="MTTEWRSYPFRLVPGDGQLEFPAAEGEHPGHESDTWFIAGRLDA
                     FEADRSFAFLAIFNKNRPGGAVVADFYTMALFDLDNGDYGTYTDYDMPPANLQPGARR
                     KLAMAEGHLDISYHGGAGTASWTTCRDGDGELIPYTYRVSLVGEDRSGRRMRLDLAVT
                     PTRAPTPVGASTYNGKIACFGQPDTYSYFQTGMAMTGTLRWGDVVQRVAGSGGHVDRQ
                     WFPKYAGGGGTGGDPRARSHEWRTINFDNGVDATIWRQFDRTNGNALQPFTGITTSYP
                     DPAQPPQCAEDVEVTVNSYVRWPNAVRPLVRPVAPARYMPDRHRISCATLQLDIVGEP
                     LVPAPAHGLPIEYMEGPYHYRGTLWGKPVTGFAFNERSLALYRDWELLEVLATTVANM
                     EPAAPHLQTMVDRVVPLVAAGRRGDAIELLAGVPPVQNETLATLLDDLIAVLAVESPD
                     HG"
     gene            6687..7409
                     /locus_tag="VFI55_07280"
     CDS             6687..7409
                     /locus_tag="VFI55_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HET7073868.1"
                     /translation="MSSVSRRDVLKFAAATPGLVGLGVAAASLCAAPASASLGTLLDY
                     AAGVIPASQIRAAGAVGSIRYVSDRRPGGTWMLGKPMQVSEARDLSTNGLKIVSCYQY
                     GKGNTADWLGGAGAGLTHAQRGLQLHSAAGGPANAPIYASIDDDPSYDQYKQQIAPYL
                     RSWESVIGHQRTGVYANSKTIDWALHDGLGSYFWQHNWGSPKGFAHPAASLHQVEIDK
                     RSVGGVGVDINEILKPQFGQWV"
     gene            7917..17165
                     /locus_tag="VFI55_07285"
     CDS             7917..17165
                     /locus_tag="VFI55_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid synthase subunit beta
                     domain-containing protein"
                     /protein_id="HET7073869.1"
                     /translation="MTIHEHDRVSADRDENSPHRTHALVDRLTAGEPYAVAFGGQGSA
                     WLETLEELVSSAGIESELATLVGEVELLLEPVAKELVVVRPIGFQPLGWVRALAAEDP
                     VPSQKHLTSAAVSIPGVLLTQIAAVRALARQGMDWSATPPVAVVGHSQGVLAVEAFNA
                     GGTHDVELLAMAQLIGAAGTLVARRRGISVLGDRPPMVSVTNADPDRIRRLLDEFAQD
                     VRTVLPPVLSIRNGRRSVVITGTPEQLSRFELYCRQISEKEEADRKNKIRGGDVFAPV
                     FDPVQVEVGFHTPRLADGIDIVGTWAEKAGLDVALARKLTEAILVNGVDWVEEITRVH
                     DAGARWILDLGPGDILTRLTAPVIRGLAVGIVPAATRGGQRNLFTVGAGPEVARAWST
                     YAPTVVRLPDGRVKLSTKFTRLTGRSPILLAGMTPTTVDAKIVAAAANAGHWAELAGG
                     GQVTEEVFAGRVEELSGLLEPGRTYQFNALFLDPYLWKLQVGGKRLVQKARQSGAAID
                     GLVVSAGIPDLEDAVELIEELNEVGISHVVFKPGTVEQIRSVIRIATEVPTKPVIMHV
                     EGGRAGGHHSWEDLDDLLLATYSELRSRPNITVCVGGGIGTPERAAEYLSGRWAQAYG
                     FPLMPIDGILVGTAAMATLESTTSPSVKRMLVETQGTDQWISAGKAQGGMASSRSQLG
                     ADIHEIDNSASRCGRLLDEVAGDAEAVAERRDEIIAAMAGTAKPYFGDIEEMTYLQWL
                     RRYVELTIGEGNSTADTAAPGSPWLADTWRERFQQMLQRAEARLNATDSGPIETLFDD
                     EALLETPDEAIATLLAQYPDAETVQLHPADVPFFVTLCKTLGKPVNFVPVIDKDVRRW
                     WRSDSLWQAHDARYEADQVCIIPGTSAVAGITLMDEPVGDLLDRFEQAAIDEVLSTDA
                     QPRAVASRRLGRPDVTGPLAVLLDAADVLWAGRTTTNPVHRIADPADWLVHDGPESLR
                     ATHSSTGARLQVNGENVVLSVPLSGAWVDIPFSLPANTVDGGTPVVSVEDATTAMRAV
                     LAIAAGVDGPEFLPAVTDGTATVTVDWDPERVADHTGVTATFGEPLAPSLTTVPDALV
                     GLCWPAVFAAIGSAVTDAGVPVVEGLLSLVHLDHAARVEGKLPKVPAQLTVTATASRA
                     TDTDMGRVVPVSVTISDADGAVIGTLDERFAILGRTGAAELTDPVRAGGAVSENATDT
                     PRRRRRDVTVAAPVDMRPFAVVSGDHNPIHTDRAAALLAGLESPIVHGMWLSAAAQHA
                     VTATDGQARPPARLVGWTARFLGMVRPGDEVEFRVDRVGIDQGAEVVEVAARVGSDLV
                     MTATARLAAPKTVYAFPGQGIQYKGMGMEVRARSKAARKVWDNADKFTRDTLGFSVLH
                     VVRDNPTSIIASGVHYHHPDGVLYLTQFTQVAMATVAAAQVAEMREQGAFVEGAIACG
                     HSVGEYTALACVTGIYEMEALLEMVFHRGSKMHDIVPRDELGRSNYRLAAIRPSQLDL
                     PDDEVPAFVAEIAERTGEFLEIVNYNLRGSQYAIAGTVRGLEELEAEVERRREIAGGK
                     RSFILVPGIDVPFHSRVLRVGVADFRRSLDRVMPRDKDPNLIIGRYIPNLVPRPFTLD
                     RDFIQEIRDLVPAEPLDPILADYDTWRAERPREMARTVFIELLAWQFASPVRWIETQD
                     LLFTEEAAGGLGVERFVEIGVKSSPTVAGLATNTLKLSEYAHSTVEVLNAERDAAVLF
                     ATDTDPEPEPEEMAEPASEPVGVGGATVVEAAAAPAPTAPSGAARPDDIGFDAADATL
                     ALIAMSAKMRIDQIEELDSIESITDGASSRRNQLLVDLGSELNLGAIEGAAEADLAGL
                     RSQVSKLARTYKPYGPVLSDAINDQLRTVLGPSGKRPAAIVERVKKTWELGDGWAKHV
                     IVEVALGTREGTSVRGGSMGHLHEGAMADAGSVDKVIDAAVTSVAARHGISVALPSAG
                     GGGGGATIDAAALSEFTDQITGRDGVLASAARLVLGQLGLDNPVSVLPAGTDAELIDL
                     VTAELGADWPRLVAPAFDAKRAVVFDDRWASAREDLVRLWLTDEGDIDADWQRLSERF
                     EGAGHVVATQATWWQGRALAAGRQIHASLYGRIAAGAENPDSGPYSNEIAVVTGASKG
                     SIAASVVARLLDGGATVIATTSKLDEERLAFYRQLYRNHARYGGALWVVAANMASYSD
                     IDALIEWIGTEQSESLGPQSIHIKDAQTPTLLFPFAAPRVAGDLSEAGSRSEMEMKVL
                     LWAVQRLIGGLSKIGAERDIASRLHVVLPGSPNRGMFGGDGAYGEAKSALDALVSRWR
                     AESSWAARVSLAHALIGWTRGTGLMGHNDAIVSAVEEAGVTTYSTDQMAAMLLNLCDV
                     ESKVAAAIAPIEADLTGGLAEAHLDMAELAAKAREQATSGEQADDIADEDTPGVIAAL
                     PSPPRGYTPAPPPHWDDLDVDPADLVVIVGGAELGPYGSSRTRFEMEVDNELSAAGVL
                     ELAWTTGLIRWEDDPQPGWYDAQSGELVDESELVERYHDTVVERCGIREFVDDGAIDP
                     DHASPLLVSVFLDKDFAFVVSSEADARAFVEFDPEHTIIRPVPDSTDWQVIRKAGTEI
                     RVPRKTKLSRVVGGQVPTGFDPTVWGISPDMASSIDRLAVWNIVATVDAFLSAGFSPA
                     EVMRYVHPSMVANTMGTGMGGGTSMQTMYHGNLLGRNKPNDIFQEVLPNIVAAHVVQS
                     YIGSYGAMIHPVAACATAAVSVEEGVDKIRLGKAELVVAGGIDDLTLEGIIGFGDMAA
                     TADTGMMRGRGIHDSKFSRPNDRRRLGFVEAQGGGTILLARGDLALRMGLPVLAVVAF
                     AQSFGDGVHTSIPAPGLGALGAGRGGKDSPLARALAKLSVSADDVAVISKHDTSTLAN
                     DPNETELHERLADSLGRSDGAPLFVVSQKSLTGHAKGGAAVFQMMGLCQMLRDGVIPP
                     NRSLDCVDDELAGSAHFVWVRETLRLGGQFPLKAGMLTSLGFGHVSGLVALVHPQAFI
                     ASLDADQRADYQRRADARVLAGQRRLASAIAGGAPMYQRPPDRRFDHHAPEKRQEAAM
                     LLNADARLGDDDSYDVSEVPVG"
     gene            17194..17586
                     /locus_tag="VFI55_07290"
     CDS             17194..17586
                     /locus_tag="VFI55_07290"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET7073870.1"
                     /translation="MGIVGVGIDLVSIPDFAEQVDQPGTAFSETFTPGERRDASDKSS
                     SAARHLAARWAAKEAVIKAWSGSRFAQRPVLREDIHRDIEVVTDMWGRPRVRLTGEIA
                     KHLADVTIHVSLTHEGDTAAAVAILETA"
     gene            17633..18958
                     /locus_tag="VFI55_07295"
     CDS             17633..18958
                     /locus_tag="VFI55_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET7073871.1"
                     /translation="MSDLVERVRDVLPSVRRDLEDLIRIESVWADPGRRDQVQRSAQA
                     VADLLSQAGFGDVSIVREGGAPAVIAKHPAPPGAPTVLLYAHHDVQPEGDHSQWTSAP
                     FEPTERDGRLYGRGSADDKAGIATHLAAFRAHGGQPPVGVTVFVEGEEESGSPSLSRL
                     LAAHRDALAADVIVIADSDNWSADVPALTVSLRGLVDCVVEVATLDHGLHSGLWGGVV
                     PDALSVLVRLLASLHDDDGNVAVAGLHESTAAEVDYPVERVRADSGLLDGVSEIGSGS
                     VPQRLWAKPAITVIGIDTTSVAASSNTLIPRARAKVSMRIAPGGDAVAHMDALAAHLQ
                     RHAPWGAHVNVTRGDMGEPYAIDASGAVYDAARAAFRQAWAVEPIDMGMGGSIPFIAE
                     FAAAFPQAKILVTGVEDPATQAHSINESLHLGVLERAAVSEALLLANLG"
     gene            complement(19015..19392)
                     /locus_tag="VFI55_07300"
     CDS             complement(19015..19392)
                     /locus_tag="VFI55_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7073872.1"
                     /translation="MTATERIIELLYRNLQEVFGEGDPARRRAAIEEFYTEDCVLYVP
                     DGVLVGHDALDKFAGELRATHPHFAYTPQGQAQALHNGGIVAWGSGPKGEPPVYTGLD
                     VVVVRESKIAALYVFLNPKLSAA"
     gene            complement(19641..20114)
                     /gene="bcp"
                     /locus_tag="VFI55_07305"
     CDS             complement(19641..20114)
                     /gene="bcp"
                     /locus_tag="VFI55_07305"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HET7073873.1"
                     /translation="MTETKRLVPGDKAPAFSLPDADGNTVSLADYQGRRVVVYFYPAA
                     STPGCTKEACDFRDNLGDLNEAGLDVVGISPDKPEKLAKFRDAEKLTFPLLSDPDRKV
                     LTAWGAYGEKQLYGKTVVGVIRSTFVVDEKGKIAVAQYNVKATGHVAKLRRDMSV"
     gene            20230..20457
                     /locus_tag="VFI55_07310"
     CDS             20230..20457
                     /locus_tag="VFI55_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003412942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HET7073874.1"
                     /translation="MAERDPETIKRDIDVARNELAATVDTLAERANPRRLADDLKART
                     VEFVKKPAVIASLVGISSVVVVVVVRRIRDR"
     gene            complement(20558..20633)
                     /locus_tag="VFI55_07315"
     tRNA            complement(20558..20633)
                     /locus_tag="VFI55_07315"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20598..20600),aa:Lys,seq:ctt)
     gene            20966..22162
                     /locus_tag="VFI55_07320"
     CDS             20966..22162
                     /locus_tag="VFI55_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020786662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET7073875.1"
                     /translation="MTPLRRRRSLLVAVMLLFAVIGVGCGTSHSAAPAKVIFDKGTPF
                     ADLLIPKLTASVADGAVGVTVDAPVTVSVADGVLGSVTMVNENGRSINGQLSPDGLRW
                     STTEQLGYNRRYTLNVKALGLGGAANRQMTFATSSPDHLTMPYVSPGDGEVVGIGAPV
                     AIRFDENIANRAAAQKAINITTNPPVEGAFYWLNNREVRWRPEHFWKPGTAIDVAINT
                     YGVDLGNGMFGEDNVKTHFSVGDEVISTADDTTKMVTVRVNGEVVKTMPTSMGKDSTP
                     TANGFYIVGSRFKHIIMDSSTYGVPVNSPNGYRTDVEWATQLSYSGVFVHSAPWSVGA
                     QGHTNTSHGCLNVSPSNAEWFYDHSKRGDIVEVVNTVGSTLSGTEGLGDWNIPWSVWK
                     AGNASA"
     gene            complement(22159..22707)
                     /locus_tag="VFI55_07325"
     CDS             complement(22159..22707)
                     /locus_tag="VFI55_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7073876.1"
                     /translation="MTRIPYRRPEDMTDRARELTLERGNLNVYRALANAENVFTGWMV
                     AGRAALTSPVLSPRLRELVVLRTACLMDCRYELGQHTDIARTVGVSEPEVKALISESN
                     WEQADFVDFTDTERAVVALASELLTTRGVSAALFDQVHQALGSEATIEVLMVISRYAG
                     LALMLNALDVDLDESARLTIPR"
     gene            complement(22722..23255)
                     /locus_tag="VFI55_07330"
     CDS             complement(22722..23255)
                     /locus_tag="VFI55_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7073877.1"
                     /translation="MSRMPLTPVEQQPAPIRDFMARRGELNLFRLLAHAPTVFAGWAQ
                     MVDEILDSPTFTPRLRELIILRVAHLQHTPYELSQHTDLARAAGLTERQLGAIVDAGD
                     ADTAGFSPTERTVLDTVTQLCTTRHLRDDSFAAVQAALGDEATTELLMIVGCYYGLAL
                     LLNAVDLDVDTTSRLQV"
     gene            complement(23267..24145)
                     /locus_tag="VFI55_07335"
     CDS             complement(23267..24145)
                     /locus_tag="VFI55_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HET7073878.1"
                     /translation="MSSNLTEQTITEAVQRSFDTSSDERFRELFVCLVQHLHDFAREV
                     RLTGDEWFTAMDFLERLGKISSPTRQEVVLLSDILGLSMLLDKINQHAAGSTTDSALL
                     GPFYVEGRPTAANGDDISRGVAGTPMFVSGRVVDEDGAPIAGVHVDTWHSDGDGFYDV
                     QQPEKLHNEFAMRALLTTDDDGLFWYRSITPRYYPVPTDGPCGEIMRAANRSIMRPQH
                     VHFWFHAKGYQTLITQLFLRDDPYIDRDAVFAAQDSLLADFVRHEPGTAPDGTLVDQP
                     FVTLDWTFVLAAETES"
     gene            complement(24184..25155)
                     /locus_tag="VFI55_07340"
     CDS             complement(24184..25155)
                     /locus_tag="VFI55_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HET7073879.1"
                     /translation="MRALRFDHFGDPSALRITDMPEPVGSEHEAVIRVEAASVNPSDV
                     KNVAGQMNWTVLPRTPGRDFAGVVIGGPPEWQGVAVWGTGGDVGFTRDGSHAELLSVP
                     VAALVRKPERLSFDEAATVGVNFVVAWYGAVETARLAAGETIAVFGVSGGVGGAVAQI
                     ARALGATVIGVDRRRPGPQTPAAAAIDDFVALDPAADTGAEIKRLTGGIGAQVVYDAV
                     GGVTTAAALASLAHRGRLVVISVVGPSAVEIDLRDLYRNETRILGIDSTQLGVVESAR
                     RLEEMGPYFESGEFRPLPVTAGYPLDEAAVAYRAVADGIAGRVVLHP"
     gene            25271..26158
                     /locus_tag="VFI55_07345"
     CDS             25271..26158
                     /locus_tag="VFI55_07345"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7073880.1"
                     /translation="MELRHLEYFVAVAAEQNFSRAARRIHVVQSALSAAVGKLEKELG
                     VPLFDRSRRQIELTAAGEVFLHHAREVLHAAHRAQSSVADHRDQLTGTVTLGTLMSWG
                     SLNLPAALEQFRRLNPRVTLRLRQSATGSAGHLAAIADGQMDLALVSIQSSPSPLVAL
                     RELTRESMVFVCESTHPLARGDRVRVADLDGEDLIQFPVGWGIRQRVDAALAAAGVQP
                     VDAYEVADYAIAAELVRHRLATAILPISAANRFPDLGKIPVYPALTWTLSLASAAPHY
                     ISRAATTLADTLGGLVDQA"
     gene            26299..26667
                     /locus_tag="VFI55_07350"
     CDS             26299..26667
                     /locus_tag="VFI55_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073881.1"
                     /translation="MKMGIARLATTLVVCGGLGLAGMGPGAASAHADDRYGPHQWCPG
                     QSMDWPSGPWDRVDWDMDVCHTWYVVGIGQGNVPERFGGPSDIWDGDNPPPPPSCAVP
                     DVPPCVMFIPGDALAFRSAR"
     gene            26867..27904
                     /locus_tag="VFI55_07355"
     CDS             26867..27904
                     /locus_tag="VFI55_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020724333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HET7073882.1"
                     /translation="MTEYRWTVIGAGPAGIATVGRLIDHGVEPDEIAWVDPEFTVGDF
                     GTNWRAVSSNTRTEGFLDFFAASPAFRFAEAPHFDIHDLDPARTCQLGLVADPLMWVS
                     GRLAEQVKTHYTVARELRLHDGHWTVATEQGDMTSANVVLAVGATQKRLDCPGLEEIP
                     LEVAVDPEKLGRRDLDGATVAVFGSSHSTMLVLPNLLATPADKVINFYRSPLKYAVEL
                     DGWTLFDDSGLKGQAACWARENIDGVQPQRLQRCQVGGSHYDELLNECDHVVYTVGFE
                     RRQLPATPQWGRLQHNPSNGIIAPGVFGVGIAFPGYRTDPLGYGEYRVGLQKFMGQLN
                     ENLPVWLRYGT"
BASE COUNT         4644 a         9269 c         9551 g         4701 t
ORIGIN      
        1 gttgactccg aacgggcccg tcgacgcaaa gatcatctac gacaactact cgctgggact
       61 gggggtcgtc cgcttcccga gcgagctggt ggccaccgtc caggtcaccg ggcagacgcc
      121 ggtcgacgag gactacaccg actacttcta cacacaggcg tccatccgcg agcccggcga
      181 caccggcgac gtgcccagcg gccgcgccgc cagatttctg gcgctgcagc aagaggtcat
      241 caaacaggac ttcttcacct gggagaacat gaaatacctg gagaagccca acctggctcc
      301 cgaagaggcg cacgactacg ccgcgctgcg ccgctgggca caccgtttct acccgggcac
      361 gcagccctcg ccctccgatt tcggctacac ccccggcggg gagcccgatc cggcggcctc
      421 gaaagtctga tcggaaaccc cgatgtgtcc cgatcggagc ctgccgttgg ggcccaccga
      481 tttcggcgtc gacaggttca ccgtgccggc tgtgctggat cgccgggccg cgcaacatcc
      541 cgacctggtg atgatgtcga tcgccggtgt cgacgtgacc ttcgagcaga tgcggcggcg
      601 gtcggccgcg gcggcaacga tgctggccga cctgggtgtc ggccgcggcg actgcgtggc
      661 gctgttcacc gccacctgcc cggaatgggt ctacttctgg ctgggcgcgg cgcgcatcgg
      721 cgcggtgagc gccgcggtga atgccgcgaa caagggtgaa ttcctgctcc acacccttcg
      781 gttggcccgg gccaaggtga tcgtcaccga cgccgaccgg cgcccgcgcg tcgacgaggt
      841 cgccgacgcg ctggagacgc tgaccgacat tgtgatgcaa gacgattcgc ttaccagtgc
      901 gttgaatctc ggcggtgact gcccaccggc ggacggccag ggcggggcgg cggacgtggg
      961 agcgctgttc tacacgtcgg gcaccaccgg gccgtcgaaa gcggttgcca cgacatggca
     1021 ctacctgttc acggtggcgg cgaccgtcgc gtcggcctgg gaattccgtc ccggggaggt
     1081 gctgtggacg gcaatgccgt tgtttcacct gagcgcggcg cccagcgtgc tggcccccat
     1141 gctggtcggc gcaacgaccg tgttggcgaa ggccttccac cccacccagg tgtgggatga
     1201 catccgtacc tgcggcgcca tcggcttcgc cggtgccggc gcgatggtgt cgatgctgca
     1261 gaatctgccg gccgatccgc gtgacgcacg cttgccgctt cggttcatct ccgccgcacc
     1321 gatcgacgcg agctcttacc gcgacatcga aaagcgttac gactgccgga tcgtcacgat
     1381 gtacgggatg accgaggcat ttccgatcgc ggtcaaaggc gtggccgacg agggggttcc
     1441 ggggacctct ggacgaccca accccaactt cgaggtgcgc atcgtcgacg aacgcggcga
     1501 tcccctgccc gccggcaccg tcggagagat cgcgtgtcga cccaggtacc cgcacgtgat
     1561 gagcgagggc tacataagcc agggtccgcg ggtcgatccg catcaggact ggtttcgcac
     1621 cggcgacctc ggccgactcg acaccgacga aagggggtac cagaacctga cgtatgtgga
     1681 tcgcatcaag gattcgttgc gcaggcgcgg cgaaaacgtc tcctcggtcg aagtggagtc
     1741 cgtcgtcatg cgccatcctg cggtggccga agccgcggcg gtcggggtgc ccagcgagct
     1801 gggtgaagac gacatcctgc tggtcgtgat tttgcgcccc ggggcgaccc tggattgcgc
     1861 agaactgctt gacttctgcg ccgctcggat gccgtatttt tgggtgcctc gattcgtcga
     1921 ggcggtcgat gaactcccga agaacggcat cggtcggata cgcaaagacc tgttgcgcgc
     1981 caggggcgta agtgccgccg cgtgggatcg tgaaaatcgg ggacacgccg ttagccggta
     2041 agttacgtta atgttacgca atcgtgggta agttacttta gtgttacatc agttccgcca
     2101 atggctgcca tccccagaga ggctggagaa caaaatgacc atcacttatc aggaagagca
     2161 attgctccag gccgcgacgg gacgtgccgc catcctggac ctgtgcgccc ggcacaaccg
     2221 ggcctattcc gacggcgatc gtgagcgctg gatcgccacg ttccgccact cgggcgcccg
     2281 ctacatacgt gacggagagg tgttcgagga tctgcgcgcg gcttttgacg gcggcgacgg
     2341 gcagcgcctg gtcacgctgg atcacgagat ccgcatcgac ggtgtgcacg ccagccaccg
     2401 ttgtgtcgtc gtgcttttcg ccgccatgta cggcgacacc acccttcggg ccaccgggac
     2461 gtaccgggac gcacttgtct acgagcgcgg cggttggtac ttcacttccc gtgcactcga
     2521 atgggattcg gtgccgagcc ggcacccgct cgtcatgtga gcgacgccga taggtggatc
     2581 gggacctcag ctaccaggta gttttcggta gctacgaaga cgcgctgcgg atggtcggcg
     2641 cgaagagcga gccgcgaacc gcgcggacac cggtcagtgg cgcgcgtatc caattgttcg
     2701 ccgcaatggt tcacgacgga aatctctcgt attgggacgc cgagttcgcc cggcggaagt
     2761 ggggaggtct gctggccccg ccggccgcgt tgatgggatg gctgatacct ccgccgtggg
     2821 agccgtcccg accaggacgt gccaggcggc cggcggcatc ggtggtgctg cgggtgccgt
     2881 tgccgggcac cacattcatc aacgcggcca acgatgttga gtttctgcag cccatcgtcg
     2941 aaggcgacct gctcacggtg gtcgaggagt tggtcgcggt gtcgcccgag aagcggactc
     3001 ggctggggat tggtcacttc gtcgagacgc tggagaccta tcggcgccag gacggggccg
     3061 tggtcgccac atgccgaaac actttgttca ggtttacgcc tgcggcggcg ccatgactgg
     3121 cgacggtctg cactgggacg cgatcgccgt tcccgtcgac ctgcccgagg tggtcgacga
     3181 gatcagctac cagcgggtgg tggagaacgc cggagcgacc tgggactatt ttcccggcca
     3241 cttcgatccc ggctatgccc gaacccaggg caacccgacg atttacctca acaccatgca
     3301 cctcgccggg ttcgccgacc gcgttgccac cgactgggcg ggcccgggca gccgcgtggt
     3361 gcgccgctcc atgcggctgg cggtcccggt ttatgccggc gacacgatgg tcggtcgggg
     3421 ccgggcggtg accaaacggc gcgacacctc cgtggacccg ccgcgctgcc ttgtcgacat
     3481 cgagatccag gtgaccaatc agcacggcac gctgtgctgc ccggtcgagc tgaccctgca
     3541 ggtgcccgaa tccttctgat cgccggcgtc gttcagccgg gtaagggcgg catctttccg
     3601 ctgcacacca ccgattgcag ctcgacgtac tcgtcgagac cctccggccc gaactcccgc
     3661 ccgaggcccg actgcttgaa accgccgaac ggcgtgctga tatcgagcat gtacatattg
     3721 atgccatagg tgccggtgcg tacctcggcg gcgatccgca gaccgcgggc ggcatcggcg
     3781 gtccacaccg aaccggccaa cccgtagtcg ctgtcgttgg cgatccggat cgcgtcgtcc
     3841 tcgtcgcggt agcgcagcac ggtcaagacg ggaccgaaga tctcctcccg cgcgatccgc
     3901 atgtcgttgg aggcgtcggc gaacagcgtc ggccgcacgt accagccccg ctcggccggg
     3961 ctgtcctcgc cgcccacgat cacgctggcg ccttcgcggc gcccggaccg gatgtagtcc
     4021 tgaacccgac gttgttgtcg ttgtgcgaca aggggtccga tgtcggtggc gtcgtcggcg
     4081 gggtccccga cgcgcagggc cgacatcatc tcggccagcg cgtcgacgaa ctcgtcgtgc
     4141 cgccgttcgc cgaccaggat gcgggtctgc gcaacgcagg cctgcccgtt gttcatcagg
     4201 cccgccgtct tcagcccggc cacggtcttg ccgacgtccg cgtcgtcgag aactatcgcc
     4261 gccgatttgc cgccgagttc caggctgacc cgcttgagct gctcgccgca caggaccgcg
     4321 atgcggcgcc cggtggcact ggaaccggtg aacgcgatct tgtccacgcc cggatggcgc
     4381 accagcgcct cgcccgcgtc cggcccgccg ggcaacaccg ataccacgcc ttcgggcaga
     4441 ccgacctgct cgatcatttc ggctaaccac aaagcgtcca acggtgtttc gggtgccggc
     4501 ttgatgacga ccgcgcagcc ggccagcaga gcgggaatca gcttgggcat gatcagacat
     4561 tgcggcacgt tccacggcac tatcgcgccg accacgccca ccggagcccg acgcagctgc
     4621 gcttcgccga gcacaccccg gcgacgctcc acccatccga agtcgcgcgc gacggtcagg
     4681 ctgaggtgga tcatcgacgc cgcgcccgcc gcctggccca gcctgctgaa actgcgcggc
     4741 gatcccatct ccgcggtgat caggtcggac atctcgtcga cgtgacgccc atagatcgcg
     4801 gccagttctt cgagcttgcc gatccgctcc ccgggagtca ttcgcggcca cggaccgtgg
     4861 tcgaaagccc gccgcgcggc ggctacggcc gcgtcgacat cctcggggcc tgccgcgggg
     4921 gcgtggccga tgggttcttc ggagtgcggg gaaatgaccg cgagctgttg cgggttggcg
     4981 ggtttgcgcc agtttccccc gatgaaaagc tcgtcatacc aacggtggcc ggggttgtcc
     5041 gtcatcgggc agctacctca gggcggggcg agaatgcaac cgtaactttg ctattgatgt
     5101 taacatagca aaaaagcacg ccgaggatgc cgacgttccc gagcaaggcg aagcgcgaga
     5161 tgaccaccga gtggcgtagt tatccgttcc ggttggtgcc cggtgacggc cagctggagt
     5221 tcccggccgc cgaaggcgaa caccccggtc acgagtcgga cacctggttc attgccggtc
     5281 ggctggacgc gttcgaggcc gatcggtcat ttgcgtttct ggccatcttc aacaagaacc
     5341 ggccgggtgg tgcggtggtc gcagacttct acaccatggc gctgttcgac ctggataacg
     5401 gcgattacgg cacctatacg gactacgaca tgccgccggc caacctgcaa ccgggagccc
     5461 ggcgcaagct ggcgatggcc gagggccatc tcgatatcag ctaccacggc ggcgccggga
     5521 ccgcgtcgtg gactacgtgt cgcgacggcg acggagaact aatcccctac acctaccggg
     5581 tcagcctggt cggcgaggac cgctctggtc gacggatgcg gctggacctg gctgtaacac
     5641 caacgcgcgc accgacgccg gtgggcgcat cgacctacaa cgggaagatc gcctgcttcg
     5701 gccaacccga cacgtattcg tatttccaga ccggaatggc gatgaccgga acattgcgct
     5761 ggggcgatgt cgtccaacgg gtcgccggaa gcggcgggca cgtcgaccgg caatggttcc
     5821 cgaaatacgc cggcggcggg gggaccgggg gagacccacg ggccagatcg cacgaatggc
     5881 gcaccatcaa cttcgacaac ggcgtcgacg ccaccatctg gcggcagttc gaccgcacga
     5941 atggcaacgc gctgcagccg tttaccggta tcaccacgag ctatccggat cccgcccagc
     6001 cgccgcagtg cgccgaggac gtcgaggtca ccgtcaacag ttacgttcgt tggccgaacg
     6061 cggtgcggcc gctggtgcgg cctgtcgctc cggcccggta catgcccgac cgccaccgca
     6121 tcagctgcgc cacgctgcag ctggacatcg tcggcgagcc gctggtgccg gccccggcgc
     6181 acggactgcc gatcgaatac atggaaggtc cgtatcacta ccgcggcaca ctgtggggca
     6241 agcccgtgac cgggttcgcg ttcaacgagc gctcgctggc gttgtatcgg gattgggagc
     6301 tgctcgaggt gctggccacc accgtcgcga acatggaacc tgccgcgccc cacctgcaga
     6361 cgatggtgga tcgggtggtg ccgttggtgg ccgcgggccg tcgcggcgac gcgatcgagc
     6421 tgctggccgg ggtgccgccg gtccaaaatg agacgctggc aacccttctc gacgacctga
     6481 tcgccgtgct ggccgtcgag tcacccgacc acggctgacc gtgccggcgc ctgggcccag
     6541 ctgaggtgac gatgctgcag ttccggttcg ttgtgctgcg ggcaggcggc agtcaaagac
     6601 ccggctccga cgttgcggca atgtgatgca gatgtgactg acgtggtcag tgtgtcttct
     6661 ggtgtttgtg tcgtaccctg tgccatatgt cttcggtctc gcggcgtgac gtgctcaaat
     6721 tcgcggccgc gaccccgggt ctggtggggc tgggagtcgc ggcggcgtcg ctgtgcgccg
     6781 cgccggcgtc ggcgtctctc ggcaccctgt tggactatgc ggccggggtc atccccgcca
     6841 gccagatcag ggccgccggg gcggtgggat cgatccggta cgtctccgat cgtcggcccg
     6901 gcggcacctg gatgctgggc aagccgatgc aggtcagcga agcgcgtgac ctgagcacca
     6961 acggcctcaa aatcgtctcg tgctaccagt acggcaaggg caacaccgcc gactggctgg
     7021 gcggagccgg cgcgggcctg acacatgcgc agcgtgggtt gcagttgcac tccgcggcgg
     7081 gtggtccggc gaacgccccc atctatgcgt cgatcgatga cgacccgtcg tacgaccagt
     7141 acaaacaaca gatagctccg tatctacggt cctgggagtc ggtgatcgga catcagcgca
     7201 cgggcgtcta cgcgaattcg aagaccatcg actgggcgct gcacgacggc ctgggctcgt
     7261 acttctggca gcacaactgg ggctcgccca agggcttcgc ccatccggcc gcgagcctgc
     7321 atcaggtcga gatcgacaaa cgcagcgtcg gcggggtcgg ggtggacatc aacgaaatcc
     7381 tcaaacccca attcgggcag tgggtctaat tcgccgacgg cgaaactcca gcaaacactt
     7441 gttcgctcat gtcgccatgc cgcgggcgcg ggcgacctcc gaatgatgct gccgacgtgg
     7501 cgtcggcggt tgcgcggcag ccgatttttt taatataacg attacgtaac aatactgcct
     7561 tgatctgcgc agttatagct actcgaccgt aaccacctgc atgcaggaag tttgttcgca
     7621 acgcccgcac cgccggttgc ggccagtgtt acccacgaca cggttacccg cgcgtagaac
     7681 tcatcagtaa ccgatcagca cgcaaaaggc gcgttgatcc ctatgacacg cttagtacag
     7741 ccgatgcagt ccgccgcacc gcgcttccac gtgggcggtc cgccgacaac acaccggcca
     7801 gccgtttccc ggctgtgatt cgacgcggaa tcgactgaaa ccgataggtc ccgccctcgg
     7861 atggtgaccg gtcgaacgaa ccgatacgta aagacgcata cagggagata cagaaggtga
     7921 cgatccacga gcacgaccgg gtgtccgccg accgcgacga gaacagcccc cacaggaccc
     7981 atgctctggt cgaccgtctg acggccggcg agccctatgc cgtcgcattt ggcggccagg
     8041 gcagcgcctg gctggagacc ctggaagaac tggtgtcgtc ggcagggatc gaatccgaac
     8101 tggccacgct ggtcggtgag gtggagctgc tgctcgagcc ggtggcgaaa gagctcgtcg
     8161 tggtccgtcc gatcggcttc cagccgctgg gttgggtgcg cgcgctggcg gccgaggacc
     8221 cggtcccctc gcaaaagcac ctgacgtcgg ccgcggtgtc gatacccggc gtgctgctca
     8281 cgcagatcgc cgccgtgcgc gccctggcgc gccagggcat ggactggagc gcgacgccgc
     8341 cggtggccgt ggtcgggcat tcgcagggcg tgctcgcggt cgaggcgttc aacgccggcg
     8401 gcacccatga cgtcgagctg ctggcgatgg cccagctgat cggtgcggcc ggcacgctgg
     8461 tcgcccgacg gcgcggaatc tcggtgctgg gcgaccgccc gccgatggtg tcggtcacca
     8521 acgccgaccc cgatcgcatc cgccgcctgc tcgacgagtt cgcgcaggat gtccgcaccg
     8581 tgctgcctcc ggtgctgtcc atccgcaacg gccgccggtc ggtcgtgatc acgggcaccc
     8641 ccgagcaact ctcgcgcttc gagctgtatt gccggcagat ctccgagaag gaagaggccg
     8701 accgcaagaa caagatccgc ggcggcgacg tcttcgcccc ggtcttcgat ccggtacagg
     8761 tcgaggtggg cttccacacg ccgcggcttg ccgacggcat cgacatcgtc ggaacctggg
     8821 ctgagaaggc cggcctcgat gtcgcgctgg cccgcaagct gaccgaggcg atcctggtca
     8881 acggcgtcga ctgggtcgaa gagatcaccc gcgtgcacga cgccggagcc cggtggattc
     8941 tggatctggg tcccggggac atcctgaccc ggctgaccgc gccggtgatc cgcggcctcg
     9001 ctgtcggcat cgtgcccgcg gccacccgcg gcggtcagcg caacctgttc accgtcgggg
     9061 ccggtcccga ggtggcccgg gcctggtcga cctacgcccc gaccgtggtt cgcctgcccg
     9121 acggcagggt caagctgtcg acgaagttca cccggctgac cgggcggtcg ccgatcctgc
     9181 tcgcgggcat gaccccgacc accgtcgacg ccaagatcgt cgccgccgcg gccaacgccg
     9241 ggcactgggc cgagctggcc ggcggcggtc aggtcaccga agaagtcttc gccggccgcg
     9301 tcgaggagct gtccggcctg ctcgagccgg ggcgcactta ccagttcaac gcgctgttcc
     9361 tggacccgta cctgtggaag ctgcaggtcg gtggcaagcg gctggtgcag aaggcccgcc
     9421 agtccggcgc ggcaatcgac ggcctcgtcg tcagcgccgg catcccggat ctcgaggatg
     9481 ccgtcgagct gatcgaggag ctcaacgagg tcggcatcag ccacgtcgtg ttcaagcccg
     9541 gcacggtcga gcagatccgt tcggtcatcc gcatcgcgac cgaggtgccc accaagccgg
     9601 tgatcatgca cgtcgagggt gggcgcgccg gcggtcacca ttcgtgggaa gacctcgacg
     9661 acttgctgct ggcgacctat tcggaattgc ggtcgcgtcc caacatcacc gtctgcgtgg
     9721 gcggcggcat cggcaccccc gagcgggcgg ccgagtacct gtccggacgc tgggcgcagg
     9781 cctacggctt cccgctgatg ccgatcgacg gcattctggt cggcaccgcg gcgatggcga
     9841 ccctggaatc gaccacgtcg ccgtcggtca agcggatgct ggtcgaaacg caaggcaccg
     9901 accagtggat cagcgccgga aaagcccagg gcggcatggc ttccagccgc agtcagctcg
     9961 gcgcggacat ccacgagatc gacaacagtg cctcgcgttg cggccgactg ctcgacgagg
    10021 tcgccggtga cgccgaggcg gtcgccgagc gccgcgacga gatcatcgcg gcgatggccg
    10081 gcaccgccaa gccgtacttc ggcgacatcg aggagatgac ctacctgcag tggctgcggc
    10141 gctacgtcga gctgaccatc ggtgagggca actcgaccgc cgataccgcc gcgccgggca
    10201 gcccctggct ggccgacacc tggcgcgagc ggttccagca aatgctgcag cgggccgaag
    10261 cgcggttgaa tgcaacggat tccgggccga tcgagacgct gttcgatgac gaggcgctgc
    10321 tggaaacccc cgacgaggcc atcgccaccc tgcttgcgca gtacccggac gccgaaaccg
    10381 tgcagctgca cccggccgac gtgccgttct tcgtcacgct gtgcaagacg ctgggcaagc
    10441 cggtcaactt cgtgccggtc atcgacaagg acgtgcggcg ctggtggcgc agtgactcgc
    10501 tgtggcaggc gcacgatgcc cgctacgagg ccgaccaggt gtgcatcatc ccgggtacct
    10561 cggcggtggc cgggatcacc ctgatggacg aacccgtcgg cgacttgctg gaccgcttcg
    10621 agcaggccgc catcgacgag gtgctgtcta cggacgcgca gccccgggcc gttgcgtcgc
    10681 gccggctggg tcgccccgac gtcaccggac cgctggccgt cctgctcgac gccgccgacg
    10741 tgctgtgggc cggccgtacc acgaccaacc cggttcaccg gatcgccgat ccggccgact
    10801 ggctggtgca tgacggaccg gaaagcctgc gcgccaccca ctcctccacg ggggcgcggc
    10861 ttcaggtcaa cggcgagaac gtcgttttga gcgtgccgtt gtccggcgcg tgggtcgaca
    10921 tcccattcag cctgcccgcc aacactgttg atggcggcac gccggtggtc tccgtcgaag
    10981 acgccaccac cgccatgcgc gcggtgctgg cgatcgccgc cggagtcgac gggccggagt
    11041 tcttgcccgc ggtcaccgac gggacggcca ccgtgacggt ggactgggac cccgagcgcg
    11101 tcgccgacca caccggagtc acggccacct tcggggagcc gctggcaccc agccttacca
    11161 ccgtgcccga cgcgctggtc ggcctgtgct ggcccgcggt tttcgccgcg atcggctcgg
    11221 cggtcaccga cgccggcgtc cccgtggtgg agggcctgct cagcctggtg catctggacc
    11281 acgccgcccg cgtggagggc aagctgccca aggtgccggc ccaattgacc gttaccgcaa
    11341 cggcgtcgcg ggccaccgac accgacatgg gccgggtagt tccggtctcg gtgacgattt
    11401 ccgacgccga cggcgcggtg atcggcaccc tcgacgagcg gttcgcgatc ctcggccgca
    11461 ccggcgccgc cgaactcacc gatccggtcc gggccggtgg cgcggtgtcc gagaacgcga
    11521 ccgacacccc gcgtcgtcgc cgtcgcgacg tcacggtggc cgcaccggtc gacatgcgcc
    11581 ccttcgcggt ggtgtccggt gaccacaacc ccatccacac cgatcgggcg gccgcgctgc
    11641 tggcgggcct cgaatcgccg atcgtgcacg gtatgtggct gtcggccgcc gcccagcacg
    11701 cggtgaccgc cactgacggt caggcccgcc cgcccgctcg gctggtcggc tggaccgcac
    11761 gattcctggg catggtgcgt cccggcgacg aggtcgagtt ccgtgtggac cgtgttggaa
    11821 tcgaccaggg cgccgaggtt gtggaagtgg ccgcgcgggt tggctcggat ctcgtgatga
    11881 cggccaccgc acggttggcc gcccccaaga cggtctacgc gttcccaggc cagggcatcc
    11941 agtacaaggg catgggcatg gaggtgcggg cccgttccaa ggcggctcgc aaggtgtggg
    12001 acaacgccga caagttcacc cgcgacacgc tgggcttctc ggtgctgcac gtggtgcgcg
    12061 acaacccgac cagcatcatc gccagcggtg tgcactacca ccaccccgac ggcgttctct
    12121 acttgacgca gttcacccag gtcgccatgg cgacggtggc ggcggcgcag gtcgccgaga
    12181 tgcgcgaaca gggcgccttc gtcgaaggtg ccatcgcctg tggccactcg gtcggcgagt
    12241 acaccgcgct ggcctgcgtg accggcatct acgagatgga agccttgctg gagatggtgt
    12301 ttcatcgcgg ctcgaagatg cacgacatcg tgccgcggga cgagttgggt cgctctaact
    12361 accggttggc ggccatccgg ccctcgcagc tggacctgcc ggacgacgag gttcctgcgt
    12421 tcgtcgccga aatcgccgag cgcacagggg aattcctgga gatcgtgaac tacaacctgc
    12481 gcggctcgca gtacgcgatc gccggcacgg tgcgtgggct ggaagagctg gaagccgagg
    12541 tcgagcggcg ccgcgagatc gccggcggca agcggtcgtt catcttggtg cccggtatcg
    12601 acgtgccgtt ccactcgcgg gtgctgcgcg tgggggttgc cgacttccgc cggtcgctgg
    12661 accgggtcat gccgcgcgac aaggacccca acctgatcat cgggcgctac atccccaacc
    12721 tggtgccgcg gccgttcacg ctggatcgcg acttcatcca ggagatccgg gatctggtgc
    12781 ccgccgagcc gcttgacccg atcctcgccg actacgacac ctggcgtgcc gagcggccgc
    12841 gcgagatggc ccgcacggtc ttcatcgagc tgttggcctg gcagttcgcc agccccgtgc
    12901 gctggatcga gacccaggat ctgctcttca ccgaagaggc cgcgggtggt ctgggtgtgg
    12961 agcggttcgt cgagatcggc gtgaagtcct cgccgaccgt cgccgggctg gccaccaaca
    13021 ccctcaagct gtccgaatat gcgcacagca cagtggaagt gctcaacgcc gaacgggacg
    13081 ctgcggtcct gttcgccacc gacaccgacc ccgagccgga gcccgaagag atggccgagc
    13141 cggcgtccga gccggttggc gttggcggag ccaccgtcgt ggaggccgca gcggcgcccg
    13201 ctccgacggc gccgtcgggt gccgcgcgcc cggacgacat cgggttcgac gccgccgacg
    13261 cgaccctcgc gttgatcgcg atgtcggcca agatgcgcat cgatcagatc gaggagctgg
    13321 actccatcga gtccatcacc gacggtgcgt cgtcgcggcg taaccagctg ctggtggacc
    13381 tgggctcgga gttgaacctc ggcgccatcg aaggggccgc cgaggccgac ctggccgggt
    13441 tgcgctcgca ggtgtccaag ctggcgcgga cctacaagcc ttacggccca gtgctttccg
    13501 acgcgatcaa cgaccagctg cggacggtcc tggggccgtc cggcaagcgg cccgccgcca
    13561 tcgtcgagcg ggtcaagaag acctgggagc tcggcgacgg atgggccaag cacgtcatcg
    13621 tcgaagtcgc gctgggcacc cgcgagggca ccagtgtccg cggcggctcg atgggccacc
    13681 tgcacgaggg cgcgatggcc gacgcgggct cggtcgacaa ggtcattgac gcggcggtca
    13741 cctccgtcgc ggcacgccac ggcatttcgg tggcgttgcc gtcggcgggc ggcggtggcg
    13801 gcggcgcgac catcgacgcg gccgcgctgt ccgaattcac cgaccagatc accgggcgcg
    13861 acggtgtgct ggcctcggcg gcccgcctgg tgctgggcca gctgggcctg gacaatccgg
    13921 tgagcgtgct gccggcgggc accgacgccg agctgatcga cttggtcacc gccgaattgg
    13981 gcgcggattg gccacgtttg gtggcgccgg cattcgacgc caagagggcc gtcgtcttcg
    14041 acgaccggtg ggccagcgcc cgcgaggacc tggtcaggct gtggctgacc gacgagggtg
    14101 acatcgacgc cgactggcag cggctgtccg agcggttcga aggtgccggc catgtcgtcg
    14161 ccacccaggc cacctggtgg cagggcaggg cgctggccgc gggccgtcag atccacgcgt
    14221 cgctgtacgg ccggatcgcg gccggtgccg agaacccgga ttcgggtccc tacagcaacg
    14281 agatcgccgt ggtgacaggc gcttcgaagg gttcgatcgc cgcgtcggtg gtcgcacgac
    14341 tgctcgacgg cggtgccacc gtcatcgcga cgacgtcgaa gctcgacgag gagcggctgg
    14401 cgttctaccg ccagctgtat cgcaaccacg cccgctacgg cggcgcgctg tgggtggtcg
    14461 ccgccaacat ggcgtcctac tccgacatcg acgcactgat cgagtggatc ggcaccgagc
    14521 agtccgaaag tcttgggcca cagtcgattc acatcaagga cgcgcagacg ccgacgctgt
    14581 tgttcccgtt cgcggcccca agggtcgcgg gggacctgtc cgaggccggt tcgcgctccg
    14641 agatggagat gaaggtgctg ctgtgggccg tgcagcggtt gatcggcggc ctgtcgaaga
    14701 tcggcgccga acgcgacatc gcctcgcggc tgcacgtggt gctgccgggc tcgcccaacc
    14761 gcggaatgtt cggcggcgac ggcgcttacg gcgaagccaa gtcggcgctg gacgcgctgg
    14821 taagccgctg gcgtgccgag tcatcctggg cggcccgggt cagcctggcc cacgccctga
    14881 tcggctggac ccgcggcacc ggcctgatgg gccacaacga cgccatcgtc agcgccgtcg
    14941 aggaggccgg tgtcaccacc tactcgaccg accagatggc ggcgatgctg ctcaacctgt
    15001 gcgacgtcga gtccaaggtg gccgcggcca tcgcgccgat cgaggcggac ctgaccggcg
    15061 gcctcgccga ggcccacctc gacatggccg aactggcagc caaagcgcgc gagcaggcca
    15121 cgtcgggcga gcaggccgac gacatcgcgg acgaggacac ccccggtgtc attgccgcgc
    15181 tgccgtcccc gccgcgcgga tacaccccgg caccgcctcc gcattgggac gacctcgacg
    15241 tcgacccggc ggatctggtg gtgatcgtcg gtggcgccga actcggccct tacggctcct
    15301 cgcgtacccg gttcgagatg gaggtcgaca acgagctgtc ggcggccggc gtcctcgagt
    15361 tggcttggac caccgggttg atccgttggg aagacgaccc gcaaccgggt tggtacgacg
    15421 cccaatccgg tgagctggtc gacgagtccg agctggtcga gcgctaccac gacaccgtcg
    15481 tcgagcgttg cggcatccgc gagttcgtcg acgacggtgc gatcgacccg gatcacgcct
    15541 cgccgctgct ggtgtcggtg ttcctcgaca aggacttcgc cttcgtggtg tcctcggagg
    15601 ccgacgcccg cgcgttcgtg gaattcgacc cggagcacac catcatccgg ccggtgcctg
    15661 actccaccga ctggcaggtc atccgcaagg ccggcaccga aattcgggtg ccgcgaaaga
    15721 ccaagctgtc acgggtggtc ggcggtcagg tcccgaccgg gttcgacccg acggtgtggg
    15781 gcatcagccc cgatatggcg agttccattg accggcttgc ggtgtggaac atcgtggcga
    15841 ccgtcgacgc gttcctgagc gccggattca gcccggccga ggtgatgcgc tatgtgcatc
    15901 ccagcatggt ggccaacacc atgggcaccg gcatgggtgg tggtacctcg atgcagacga
    15961 tgtaccacgg aaacctgttg ggccgtaaca agccgaacga catcttccag gaagtgctgc
    16021 cgaatatcgt tgccgcgcat gtggttcagt cctacatcgg cagctacggc gcgatgatcc
    16081 acccggtcgc cgcgtgcgcg acggccgcgg tgtcggtcga ggagggcgtc gacaagatcc
    16141 ggctgggcaa ggccgagctg gtggtggccg gcggcatcga cgacctgacg ctggagggca
    16201 tcatcggctt cggtgacatg gcggccaccg ccgataccgg catgatgcgc ggcaggggca
    16261 tccacgactc gaagttctcc cggcccaacg accggcgccg gctgggcttc gtcgaagccc
    16321 agggtggcgg gacgatcctg ttggcgcgcg gtgacctggc gctcaggatg ggtctgccgg
    16381 tgctcgcggt ggtggcgttc gcgcagtcgt tcggcgacgg cgtgcacacc tcgatcccgg
    16441 cccccggtct gggtgcgttg ggcgcgggcc gcggtggtaa ggactcgccg ctggcgcggg
    16501 cgctggccaa gttgagtgtt tccgcggacg acgtcgcggt gatctccaag cacgacacct
    16561 cgacgctggc caacgatccc aacgagacgg agctgcacga gcggctggcg gactcgctgg
    16621 gccgctcgga cggtgcaccg ctgttcgtgg tgtcgcagaa gagcctgacc ggccatgcca
    16681 agggcggtgc ggcggtcttc cagatgatgg gcctgtgcca gatgctgcgc gacggggtga
    16741 tcccgcccaa ccgcagcctg gattgcgtcg acgacgaact ggccggctcg gcccacttcg
    16801 tctgggtacg cgagacgctg cggctcggcg gccagttccc gctcaaggcc ggcatgctga
    16861 ccagcctcgg gttcggccac gtgtccggtc tggtcgcgct ggtgcacccg caggccttca
    16921 tcgcctcgct ggatgcggat cagcgtgcgg actaccagcg gcgcgcggac gcgcgcgtgc
    16981 tggccggaca gcgcaggctg gcctcggcca tcgccggcgg tgcgccgatg taccagcggc
    17041 caccggaccg tcggttcgac catcacgcgc cggagaagcg ccaggaggcg gcaatgctgc
    17101 tgaacgctga cgctcggctg ggtgacgacg actcctacga cgtcagcgag gtccctgtcg
    17161 gatgacttcg gccatccgtt aacctggcca gccatgggca ttgtcggcgt ggggatcgac
    17221 ctcgtctcga tacctgattt cgctgagcag gtcgaccagc cgggcacggc gttctcggag
    17281 acgttcactc cgggtgagcg ccgcgacgcc tcggacaaga gctcgtcggc ggcgcggcac
    17341 ctggccgcgc gctgggccgc caaggaggcg gtgatcaagg cctggtccgg gtcgcggttc
    17401 gcccaacggc cggtgttgcg cgaggacatc caccgcgaca tcgaggtggt caccgacatg
    17461 tggggacgac cgcgggtgcg gttgaccgga gagatcgcca agcatctggc cgacgtgacg
    17521 atccacgtgt cgctgacgca cgaaggcgac accgcggccg cggtggccat cctggagact
    17581 gcgtgatcgc gagcgtgcgg tgcccctgct cgggtgaact agcctcgacg ccatgagtga
    17641 tctcgtcgaa cgtgtccgcg atgtgttgcc gtcggtgcgc cgcgatctcg aggacctgat
    17701 ccgcatcgaa tcggtgtggg ccgaccccgg ccggcgcgac caggtgcaac gcagcgcgca
    17761 ggcggtcgcg gatctgttgt cgcaggcggg ctttggcgat gtcagcatcg tccgcgaggg
    17821 cggtgcgccc gccgtcatcg cgaagcatcc ggcgcccccg ggcgcgccca cggtgctgct
    17881 gtacgcccac cacgatgtgc agcccgaggg cgatcacagc cagtggacgt cggcgccgtt
    17941 tgagcccacc gaacgcgatg gccggctcta tggccgcggc agcgcggacg acaaggccgg
    18001 catagcaacg catttggcgg cgtttcgcgc ccacggcggc caaccgccgg tcggtgtgac
    18061 ggtcttcgtc gaaggcgagg aggagtccgg ctcgccgtca ctgagccggt tgcttgccgc
    18121 ccaccgcgac gcgctggccg cggacgtgat cgtgatcgcc gactcggaca actggagcgc
    18181 ggacgtcccg gcgctgacgg tttcgctgcg cggcctggtc gactgcgtgg tcgaggtcgc
    18241 cacgctcgac cacgggctgc actcggggtt gtggggcggc gtggttcccg acgcgctgag
    18301 cgtgctggtg cggctgctgg ccagcctgca cgacgacgac ggcaacgtgg cggtggccgg
    18361 cctgcacgaa agcacggccg ccgaggtcga ttacccggtg gagcgggtcc gcgccgactc
    18421 cggtctgctg gacggggtgt ccgagatcgg ctcgggctcg gtgccccaac ggctgtgggc
    18481 caaaccggcg atcaccgtga tcggcatcga caccacgtcg gttgcggcct catcgaacac
    18541 gctgatcccg cgggcgcggg ccaaggtcag catgcggatc gctcccggcg gtgacgcggt
    18601 ggcgcacatg gacgcgttgg cggcgcacct gcagcggcac gcgccgtggg gcgcccatgt
    18661 caacgtcacg cgtggcgata tgggcgagcc ctacgccatc gacgccagcg gcgcagtcta
    18721 tgacgccgca cgggcggctt ttcggcaggc gtgggctgtc gagccgatcg acatgggcat
    18781 gggcggctcg atcccgttca tcgcggagtt cgccgccgcg ttcccccagg cgaagatcct
    18841 cgtcaccggc gtcgaagacc ccgcgacgca ggcgcacagc atcaacgaga gcctgcacct
    18901 gggcgtgctg gaacgcgcgg cggtcagcga agcgctgcta ctggccaacc tgggctgact
    18961 aaaccacagg ccgcggccaa gtttagcgac gtggccgctg gccttgatgt tgtatcaggc
    19021 agcggacaat ttcgggttga gaaacacgta cagcgcggcg atcttgctct cgcggacaac
    19081 gacaacatcg agcccggtgt acacgggcgg ctcgcctttc gggcccgagc cccaagcaac
    19141 aattcccccg ttgtgtagag cctgggcctg tccctggggt gtgtaggcga aatggggatg
    19201 ggtcgcgcgc agttccccgg cgaatttgtc cagtgcgtcg tgccccacca ggacgccgtc
    19261 tggaacgtag agcacgcagt cttctgtgta gaactcttcg atggcggcgc gccggcgggc
    19321 cggatcgccc tccccgaaga cctcctgaag gtttcgatag agaagttcga taattcgctc
    19381 agtcgcagtc atattcgcgc cgcccttctt cgaggtgccg tcttgtccaa caccttcggt
    19441 gatgctaaac ggactatggt ccgatttaat tcctctccgc gtcgaatcag ctcgacagag
    19501 tgatgacgag gcgccgagaa ctccctgcct catagtccat cgagatgttc tcgatgccgt
    19561 ggcagttcgg tcgcggagtc agacaccgcg aaatccccag agcgagagga tatctacgtc
    19621 caggcggtcc agccgtctac ctaaaccgac atgtcgcgcc gcagcttggc cacatggcca
    19681 gtggccttga cgttgtactg cgcgacggcg attttgcctt tttcgtcgac cacgaaggtg
    19741 gaacgaatca cacccacgac ggtcttgcca tacagctgct tctcgccgta ggcgccccag
    19801 gccgtcagca ccttgcggtc gggatcggag agcagcggga acgtcagctt ctcggcgtcc
    19861 cggaacttgg cgagcttctc cggcttgtcg ggggagatcc ccaccacgtc aaggccggct
    19921 tcgttgaggt cacccagatt gtcgcgaaaa tcgcaggcct ctttagtgca tcccggcgtt
    19981 gaggcagcgg ggtagaaata cacgacgacg cggcgccctt ggtaatcggc cagcgacacc
    20041 gtgttgccgt cggcgtcggg caggctgaag gcgggcgctt tgtcgcccgg caccagccgc
    20101 ttggtctcgg tcaactcggc tcctttctgt tcaccggagc gatcggatca gcgtcagctg
    20161 atctagggta gtgcctcagg tgtatcagcc aggcttgccg gctgacaggg cggacttgga
    20221 ggaacagacg tggcggagcg ggatcccgag accatcaagc gtgatatcga cgtcgcccgc
    20281 aacgagctgg cggccaccgt cgataccctt gctgagcgtg cgaaccctcg ccgcctcgcc
    20341 gacgatctga aagcccgcac ggtcgaattc gtgaagaagc ccgcggtgat cgcctcactg
    20401 gtgggaatct cgtcggtcgt cgtggtcgtg gtggttcgga ggatcaggga tcgctgaccc
    20461 cggccctgcg cagcggcggc cacgccacga tgcagacgcg ccgaagcggg tagcggacac
    20521 cgggtagggg acatgaagcg ccagaaacgc tggcttgtgg tgcgccgtca gggtttcgaa
    20581 ccccggaccc gctgattaag agtcagctgc tctaccaact gagctaacgg cgcggtgctg
    20641 gccaagaccg cgtttgcgac aataacaggc gttgtgtcgc agagcgaaat tcctggaccc
    20701 aacccagtct agatcctccg tcgccggcat cctcccggcg tttgcgggac gccccgacgc
    20761 tcgcttaagg tcagtgggtt gtagcaaaat cgctgaaccg gccggcgggt cacgatttgg
    20821 tgtgacattg gtcgcgatgg cggcaaaggc ccagacaata ccgttcatgt tggcgcgatg
    20881 gacggccgca aaccagtaaa atcctttgga atcattcagg gtcagcggtg accaaaataa
    20941 gcaaggtgat gtgaaaggtc acttgatgac gcctttgcgc agacgtcgat cgttgctggt
    21001 tgccgtgatg cttttgttcg ctgtgatcgg cgtcggttgc ggcaccagcc acagcgccgc
    21061 gccggccaag gtcattttcg acaagggcac gccgtttgcc gatctgctga tccccaagct
    21121 caccgcgtcg gtggccgacg gcgccgtcgg cgtcacggtg gacgcgccgg tcacggtgag
    21181 tgttgccgac ggcgtgctcg gctcggtgac catggtcaac gagaacggca ggtccataaa
    21241 cggccagctc agcccggacg ggctgcgctg gtcgaccacc gagcagcttg gctacaaccg
    21301 gcgctacacg ctgaacgtga aggcgctggg gctgggcggc gccgcgaacc ggcagatgac
    21361 cttcgcgacc agttcacccg atcacctgac catgccctac gtgagtccgg gcgacggcga
    21421 ggtggtgggc atcggtgcgc cggtggcgat ccgcttcgac gagaacatcg cgaaccgcgc
    21481 ggcagcccag aaggccatca acatcaccac caacccgccc gtcgagggcg cgttctactg
    21541 gctgaacaac cgcgaagtac gttggcgccc agagcatttc tggaagccgg gaaccgcgat
    21601 tgacgtggcg atcaacacct atggcgtcga tttgggcaac gggatgttcg gcgaggacaa
    21661 cgtcaagacc cacttttccg tcggcgacga ggtgatctcg accgctgacg acaccaccaa
    21721 gatggtgacc gtgcgggtca acggcgaagt cgtcaagacc atgccgacgt cgatgggcaa
    21781 ggacagcacc ccgacggcca acggcttcta catcgtcggt tcgcggttca agcacatcat
    21841 catggactcc tcgacctacg gggtgcccgt caactcgccc aacggctatc gcaccgatgt
    21901 tgagtgggcc acccagctgt cctacagcgg cgttttcgtg cactccgcgc cgtggtcggt
    21961 gggcgctcaa ggccacacca acaccagcca tggttgtctg aatgtgagtc cgagcaacgc
    22021 cgagtggttc tacgaccaca gcaagcgcgg ggacatcgtc gaggtcgtga acacggtggg
    22081 ttcgacgctt tcgggtaccg agggtctcgg tgactggaat atcccgtggt cggtgtggaa
    22141 ggcgggcaac gccagcgcct aacgagggat ggtcagccgc gcgctttcgt ccaggtcgac
    22201 gtcgagcgcg ttgagcatca gcgccagacc cgcgtaccgg ctgatcacca tgagcacttc
    22261 gatggtggcc tcggagccga gcgcttggtg cacctgatcg aacagtgcgg ccgagacgcc
    22321 gcgggtggtc aacagttcgg atgccagggc aaccaccgcg cgttcggtgt cggtaaagtc
    22381 gacgaaatcg gcttgttccc agtttgattc ggagatcagg gccttgacct ctggctcgct
    22441 gaccccgacc gtgcgcgcga tgtcggtgtg ctggccgagt tcatagcggc agtccatcag
    22501 acaggccgtg cgcaggacga cgagttcgcg cagcctgggg gagagcacgg ggctggtcag
    22561 ggcggctcgt ccggccacca tccagccggt gaagacgttc tcggcgttgg ccagggcgcg
    22621 gtagacgttg agattgccgc gctccagggt cagttccctg gcccggtcgg tcatgtcttc
    22681 gggccggcga taagggattc gggtcatggt cgctctcctg ttcacacctg cagccgggat
    22741 gtggtgtcga cgtcgaggtc gacggcgttg agcagcagcg ccagcccgta gtagcagccg
    22801 acgatcatca gcaattcggt ggtcgcctcg tcgccgaggg ccgcctggac cgcggcgaac
    22861 gaatcgtcgc ggaggtgccg agtcgtgcac agctgggtca ccgtatcgag cacggtgcgt
    22921 tcggttgggc tgaaccccgc cgtgtcggca tcgccggcgt cgacgatggc gccgagctgt
    22981 cgttcggtca gacccgcggc acgggccagg tccgtgtgct ggctcaactc gtatggggtg
    23041 tgctgcaaat gggccacccg caggatgatc aactcgcgta gccgtggggt gaaggtgggg
    23101 ctgtcgagga tttcgtcgac catctgcgcc catccggcga acactgtcgg ggcgtgggcg
    23161 agcagccgga agaggttcaa ctcgccgcgg cgtgccatga agtcgcggat cggtgcgggt
    23221 tgctgctcga cgggggtcag gggcatgcgc gacatcggct tgcctcctac gactctgtct
    23281 cggcggcgag gacgaaggtc cagtccaggg tgacgaacgg ctggtcgacg agtgttccgt
    23341 ccggcgcggt gccgggctcg tgccgcacga agtccgccag cagcgagtcc tgcgcggcga
    23401 agaccgcgtc gcggtcgatg taggggtcgt cgcgcaagaa caattgcgtg atcagcgtct
    23461 ggtaaccctt ggcgtggaac cagaagtgca catgttgcgg gcgcatgatg gatcggtttg
    23521 cggcgcgcat gatctccccg caagggccgt cggtgggcac cgggtaatag cgaggcgtga
    23581 tggaccggta ccagaacagg ccgtcgtcgt ccgtggtcag cagcgcccgc atggcgaact
    23641 cgttgtgcag cttctcgggc tgctgcacgt cgtagaaccc gtcaccgtcg ctgtgccagg
    23701 tgtccacgtg gacaccggca atgggcgcgc catcttcgtc gacgacccga ccgctgacga
    23761 acatcggcgt ccccgcaacg ccgcgggaga tatcgtcccc gtttgccgcg gtcggacggc
    23821 cctcgacgta gaacgggccg agcagggccg agtcggtggt cgatcccgct gcgtgctggt
    23881 tgatcttgtc cagcagcatg ctcaatccca ggatgtcgga cagcaacacc acttcctgac
    23941 gggttgggct ggagattttg ccaagccgct caaggaaatc catggcggtg aaccattcgt
    24001 ctccggtcag ccgcacctcg cgcgcgaaat cgtgcaggtg ctgcaccagg cagacgaaca
    24061 actcgcggaa gcgctcgtcg ctagacgtgt cgaaggaccg ctggacggcc tcggtgatgg
    24121 tttgctcggt gagattgctc gacatggcca tccctctcgt tgaaattggc tgttcggatg
    24181 aggtcagggg tgcaagacga cgcggccggc tatgccatcg gcgacggcgc gatatgccac
    24241 ggccgcttcg tcgaggggat agccggcggt gacggggagc ggccggaatt cgccggattc
    24301 gaagtagggc cccatctctt ccaggcgccg cgcggattcc accacgccga gctgcgtgct
    24361 gtctatgccc aagatccggg tttcgttacg gtacaggtcc ctaaggtcaa tctcgacggc
    24421 gctgggaccg acgacgctga tgacgaccag cctgccgcgg tgggccagtg aagccagcgc
    24481 cgccgccgtg gtcaccccgc ctaccgcgtc gtacaccacc tgcgctccga ttccgcccgt
    24541 caggcgcttg atctcggcac cggtgtcagc ggccgggtcc aaggcgacga agtcatcgat
    24601 cgcggccgcg gcgggagtct gtgggccagg ccggcgccgg tccaccccga tcacggtggc
    24661 gccgagcgct cgcgcgatct gggccaccgc gcccccgacc ccgccggaga cgccgaagac
    24721 ggcaatggtt tcaccggcgg ccagccgggc ggtttccacc gcgccgtacc acgccaccac
    24781 gaagttcact cccacagtgg cggcttcgtc gaagctcagc cgttccggtt tgcgcaccag
    24841 tgccgccacg ggcaccgaga gcagctcggc gtgcgagccg tcgcgggtga agccgacgtc
    24901 tccgcccgtg ccccacaccg caactccttg ccattcgggt gggccgccga tgaccacacc
    24961 ggcgaagtcg cgcccgggcg tgcgcggcag cacggtccag ttcatttggc cggcaacatt
    25021 tttcacatcg gacgggttga ccgaagcggc ctcgacacga atgaccgcct catgttctga
    25081 tcccaccggc tcgggcatgt cggtgatccg tagggcggag gggtcgccga aatggtcgaa
    25141 acgcagagcg cgcatgtcac gtcctcctgt gggctgatcg tctgacacgt ccagacaaca
    25201 cccgataaac gatcgcgtcc aacgaagaaa gccgatcatg attatcgcgt ctcgcgatag
    25261 gattggctgg atggaactgc ggcatctcga atacttcgtc gcggtggccg ccgagcagaa
    25321 cttctcccgc gcggcccggc gcatccacgt cgtccagtct gcgctgtcgg cggcggtggg
    25381 caaactggaa aaagaactgg gcgtaccgct tttcgaccga tccaggcgcc agatcgagct
    25441 caccgcggcc ggggaagtgt tcctgcatca cgcccgtgaa gtgctgcatg cggctcatcg
    25501 agcccaatcg tcggtcgccg atcaccgtga tcagctgacg ggaacggtca cgctcggcac
    25561 cctgatgtcg tggggatcgc tgaacctgcc ggcggccctg gagcagttcc gtcgcctcaa
    25621 cccgcgggtg acgttgcgcc tgcgacaaag cgcgaccggc tcggccgggc atctggccgc
    25681 gatcgccgac ggacagatgg acctggcgct ggtgtccatc cagtcgtcgc cgtctccgct
    25741 tgtggcgttg cgtgagctca cgcgagagtc catggtgttt gtctgcgagt ccacccaccc
    25801 actggcccgc ggcgatcgcg tgcgggttgc cgacctggac ggagaagatc tcatccagtt
    25861 cccggtcggg tggggaatcc ggcagcgcgt cgatgctgct ctcgccgcag cgggcgtcca
    25921 gccggtcgat gcttacgaag tggcggatta cgcgatcgcc gccgagctgg tccgacaccg
    25981 gctggcaacg gcgatcctgc ccatcagcgc ggcaaaccgg ttcccggact tgggcaaaat
    26041 ccccgtgtac ccagccttga cgtggacatt gtcactggcc tctgctgcgc cccattacat
    26101 cagccgggcc gcgacgaccc tcgccgacac ccttggcggt ctcgtcgacc aggcctaaac
    26161 aggcgacagt cccgatgtcg cacaacggtt ttggcgtggc accgacgttg tcgcagccga
    26221 cgccgacaac actggccaat gagcggactt tgccgattcg tgtgcctttc ctgttcgccc
    26281 ggcaatacag tgatgtccat gaaaatgggc atcgcgcggt tagctacgac cctggtggtg
    26341 tgtggcggat tggggctggc gggtatgggg ccgggtgcgg ccagcgctca cgccgacgac
    26401 cggtacggcc cgcaccagtg gtgccccggg cagtcgatgg actggccgag tggaccctgg
    26461 gaccgcgttg attgggacat ggacgtgtgc cacacctggt atgtcgtcgg catcgggcag
    26521 ggcaatgttc ccgagcgttt tggcggcccc agcgatattt gggacggcga caatcctccg
    26581 ccgcccccgt cttgcgcagt ccccgatgta cctccatgtg tgatgtttat cccgggcgac
    26641 gcacttgcct tccggtccgc acggtgaggt gatcacgacc tggcgcgcat agcgccctgc
    26701 gtccggtcgg ccacaagccg cctttgttgg tccacaagaa cttggcctct gcccgatgcc
    26761 gtcatagcgg cgaagtatca aaagtgccaa cacattcatg cagttagcag cgatgtcgac
    26821 ctcgcgcaga cggcgccgct cgactcgtca gacgaggaga ttcaccatga ccgaataccg
    26881 ctggactgtc atcggagcag ggcccgccgg gatcgccacg gtcggcaggc tgatcgacca
    26941 cggcgtcgag cctgacgaaa tagcctgggt tgaccccgag ttcacggtgg gcgacttcgg
    27001 aaccaattgg cgagcggtct ccagcaacac acgcaccgag gggtttctcg acttcttcgc
    27061 ggcgtcgccg gcgttccgct tcgccgaggc accgcatttc gacatccacg accttgatcc
    27121 cgcgcgaacg tgtcaactgg gcctggtggc cgatccgctg atgtgggtat cgggcaggct
    27181 ggctgagcaa gtcaagacgc actacaccgt cgcgcgggag ctgcgtctgc atgacgggca
    27241 ctggaccgtg gccaccgagc agggcgacat gacgtcggca aacgtggttc tcgcggttgg
    27301 tgcgacgcag aaacgacttg actgccccgg acttgaggaa atccccctgg aagttgcggt
    27361 ggaccccgag aagcttggtc ggcgggacct ggacggggct accgtcgccg tcttcggctc
    27421 atcacactca acgatgctgg tgctgccgaa tcttctggca acgcccgccg acaaggtcat
    27481 caacttctac cgcagccccc tcaagtacgc cgttgaactc gacggctgga cgctgttcga
    27541 cgacagcggc ctcaaagggc aagccgcatg ttgggcccgc gagaacatcg atggggtcca
    27601 accccaacgg ctgcaacgct gccaggtcgg cggttcgcac tacgacgaac tgctcaatga
    27661 gtgcgaccac gtggtctaca ccgtcggatt cgagcgtcgg cagcttccag caacgccgca
    27721 gtggggtcgc ctgcagcaca acccatccaa cggaatcatc gctccgggag ttttcggcgt
    27781 cggcatcgcc ttccccgggt atcgaaccga tccgctgggc tacggcgagt accgcgtcgg
    27841 attgcagaag ttcatggggc aactcaacga aaacctaccg gtgtggttgc gttacggcac
    27901 ctgaagcgtg tgccacggag tttgtgcttg accccaccaa cagaggggtg aggctcaatg
    27961 tttcaggaac ggtagcccac gggcaccatg atcggccgta gtccctgatc gaccaaccgc
    28021 cttggttatg ccgcgaggct ggccgcccag gcatggacct cagcatcact gcccataact
    28081 tgtgtcttga tcgtcatgga aggcgcgtag agcgtgccca cccagcgacc catctcaagg
    28141 tccacccgca gcaccgaacc gttgg
//
LOCUS       DASSGQ010000164         6248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_467684,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000164 DASSGQ010000000
VERSION     DASSGQ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467684"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VFI55_07360"
     CDS             complement(<1..735)
                     /locus_tag="VFI55_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7073883.1"
                     /translation="MPVQNIIAGTGAESIAANVEEAMSRGALAPGDALPPIRELADQL
                     GVNANTVAAAYRLLRDRGAVETAGRRGTRVRHRPATTPRSLLGLDIPEGVRDLSTGNP
                     DPALLPIALARVPQARPVLYGEPATLPELAEFSRVALSADGIPADHLAVTSGALDGIE
                     RALTAHLRPGDRVAVEDPGWANLLDLLAALGLSVEPVRVDDDGPLPADMTRALSRGVR
                     ALVVTNRAQNPTGAALSADRADALRTL"
     gene            776..1435
                     /locus_tag="VFI55_07365"
     CDS             776..1435
                     /locus_tag="VFI55_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020725054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET7073884.1"
                     /translation="METAYRPTHRTTLTRHRERAQYERTTIHEILDEALICHLGYLSG
                     GRPVVLPTTHARLGETLYLHGSTGGGPLLAAMAANASGSDLPVCVTVTLVDGLVLARA
                     AMHHSVNFRSVVVLGAARVVDDPAEKLRALSCLLDHIRPGRAADCRTPSARELAATGV
                     LALDLAEVSAKVRTGGPVDEPEDTTLPHWAGVVPLSLTAGDPVPADDLDPATPLPAYL
                     R"
     gene            complement(1457..2737)
                     /gene="ilvA"
                     /locus_tag="VFI55_07370"
     CDS             complement(1457..2737)
                     /gene="ilvA"
                     /locus_tag="VFI55_07370"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HET7073885.1"
                     /translation="MSAELSRSPSMSPLCAADIDSAARRIAPVVTPTPLQFSDRLSAI
                     TGAQVYLKREDLQTVRSYKLRGAYNLLVQLTDEEMAAGVVCSSAGNHAQGFAYACRKL
                     GVRGRVYVPAKTPKQKRDRIRYHGGEFIELIVGGSTYDFAADAALEDVKRTGATLVPP
                     YDDLRTMAGQGTIAVEVLDQLGAEPDLVVVPVGGGGCIAGITTYLAERTTSTAVLGIE
                     PAGAAAMMAALAAGEPVTLDFVDQFVDGAAVRRAGTLTYATLAAAGDMVSITTVDEGA
                     VCTAMLDLYQHEGIIAEPAGALSVAGLLEADIEPGSTVVCLISGGNNDVSRYGEVLER
                     SLVHLGLKHYFLVDFPQEPGALRRFLDDVLGPNDDVTLFEYVKRNNRETGEALVGIEL
                     GSAADLDGLLARMRATEIQIEALEPGSPAYRYLL"
     gene            complement(2787..3224)
                     /locus_tag="VFI55_07375"
     CDS             complement(2787..3224)
                     /locus_tag="VFI55_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET7073886.1"
                     /translation="MPLSGEYAPSPVDWAREQADKYVESGGTVGTDMQGKPVILLTTV
                     GAKSGKLRRTPLMRVEHDGEYAIVASLSGAPKNPVWYYNVVANPRVELQDGTVTRDYD
                     AREVFGDEKAAWWERAVEVWPDYAGYQKKTDRQIPLFVLTPVS"
     gene            complement(3274..5175)
                     /locus_tag="VFI55_07380"
     CDS             complement(3274..5175)
                     /locus_tag="VFI55_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase"
                     /protein_id="HET7073887.1"
                     /translation="MTVREIGRVGLRKVLQRTGVIDESTAPLPTDPVEVVQLLESPWY
                     DERLVELANELERDPDSVRAEAAGYLREMSAELDDRAVRAWRGFSRWFMRAYDVLVDD
                     DQIAQLRKLDRKATLAFAFSHRSYLDGMLLPEVIQANRLSPALTFGGANLNFFPMGVW
                     AKRTGTIFIRRDTKDIPVYRFVLRAYVAQLVQNHDNLTWSIEGGRTRTGKLRPPVYGI
                     LRYITDAVDEIDGPEVYLVPTSIVYDQLHEVEAMTTEAYGATKRPEDLRFLIRLARQQ
                     GERLGRAYVDFGEPLPLRKRLEELRAEESGTGTEIERIALDVEHRINRATPVTPIAVV
                     NLALLGADRSLSISEVLATVRPLASYIAARNWAVAGAADLTNRSTIRWTLHELVASGV
                     ISVYDAGTEAVWGIGADQHLVAAFYRNTAIHILVDRAIAETALLAAAENAETTMEGSV
                     QPGTVRDEALNLRELLKFEFLFSARAQFEKDLADEVRLIGPVEDITKPVSAAYVRRLL
                     EDADLLLAHLVLRPFLDAYHIVADRLAARESGSFDEKAFLTECLEVGKQWELQRRIAS
                     AESRSMELFKTALRLARHRELVDGFDDPEIAERRRQFADEIATAVRRVNTIAELARPA
                     AKSGVASES"
     gene            5760..>6248
                     /locus_tag="VFI55_07385"
     CDS             5760..>6248
                     /locus_tag="VFI55_07385"
                     /inference="COORDINATES: protein motif:HMM:NF042532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protealysin inhibitor emfourin"
                     /protein_id="HET7073888.1"
                     /translation="MEITIERCGGLIGQAERLGPVDTAALPQDVARKIDAIISEMKFF
                     DLPVTITKPGGADLVEYRTIVADGSRTHTVYSNELSDAAYQMELGDLIGLLEAAGFKF
                     VVIELGITSKIAMEASRYDLTGGGITLEYFTFANLNKERILWYTDGRRTLEFYENDVR
                     TAD"
BASE COUNT         1061 a         2132 c         2008 g         1047 t
ORIGIN      
        1 caacgtacgc agcgcatccg cccgatccgc cgacagcgcc gccccggtcg ggttttgcgc
       61 tcgattcgtg acgaccagcg cgcgcacacc gcgactcagc gcccgggtca tgtcggcggg
      121 cagcggcccg tcgtcgtcga cgcgcacggg ctcaaccgaa agaccaagcg cggcaaggag
      181 atccaacagg ttggcccagc ccggatcctc gacggccacg cggtcaccgg ggcggagatg
      241 cgcggtgagc gcccgctcga tgccatccag cgcaccgctg gtcaccgcca ggtgatcggc
      301 cggaatgcca tcggccgaca gcgccacgcg gctgaactcg gccagttcgg gcaacgtggc
      361 cggctccccg tacagcaccg gccgggcctg cgggacccgg gccaacgcga tcggcaacag
      421 ggccgggtcc gggttgccgg tcgacaggtc gcggaccccc tccgggatgt cgagcccgag
      481 cagggagcgc ggcgtggtcg ccgggcggtg ccgcacccga gtgccgcgcc ggcccgcggt
      541 ttccacggcc ccgcgatcac gcagcaggcg atacgccgcg gccacggtgt tggcgttcac
      601 cccgagctgg tcggccagct cgcggatcgg cggcaatgca tcaccgggcg ccagggcgcc
      661 acgggacatg gcctcttcga cgttggcggc tatggactcc gcgcccgtcc ccgctatgat
      721 gttttgtact ggcacacaca tgattatgta ctagaacaaa tatgaaggca atccattgga
      781 aacggcatac cgtcccacgc accgaacgac gctcacccgc catcgggagc gcgctcagta
      841 cgagcgcacc acgatccacg agatcctcga cgaggccctg atctgccact tgggctacct
      901 gagcggcggc aggccggtgg tgttgccgac gacgcacgcc cgcctcggcg agactcttta
      961 cctgcacggt tccaccggcg gcggcccgct gctcgcggcc atggccgcca acgcgtcggg
     1021 gtcggacctg cccgtctgcg tcaccgtcac cctcgtcgac gggctcgtgc tggcccgcgc
     1081 ggcaatgcac cattcggtga actttcgctc ggtggtcgtg ctgggcgcag cacgcgtggt
     1141 ggacgatccg gcggaaaagt tgcgggcgct aagctgcctg ctcgaccaca tcaggcctgg
     1201 ccgcgcggcg gactgcagga cgccgagcgc gcgtgaactc gccgccaccg gggtgctcgc
     1261 cctcgacctc gccgaggtct cggccaaggt gcgcaccggc ggcccggttg acgagccaga
     1321 ggacaccacc ctgccgcact gggcgggcgt ggtgccgctg agcctgacgg ccggagatcc
     1381 ggtgccggcc gacgacctcg accccgctac cccgctgccg gcctacctgc gctagccgcg
     1441 gcgagcgtgc gggtcgctag agcaggtagc ggtacgccgg agacccgggc tcaagcgctt
     1501 cgatctgaat ctccgtcgct cgcatccggg ccagcagccc gtccaggtcc gcggccgatc
     1561 ccagctcgat gccgaccagc gcctcaccgg tctcccggtt gttgcgcttg acgtactcga
     1621 acagggtgac gtcgtcgttg ggtccgagca cgtcgtcgag gaaccggcgc aaggcgccgg
     1681 gttcttgcgg gaagtccacc aggaagtagt gcttgaggcc gaggtgaacc agcgagcgct
     1741 ccagcacctc gccgtagcgc gacacgtcgt tgttgccgcc cgagatgagg cagaccacgg
     1801 tcgacccggg ttcgatgtcg gcttccagca ggcccgcgac cgacagcgcg cccgccggtt
     1861 cggcgatgat cccctcgtgt tgatagaggt ccagcatcgc ggtgcacaca gcgccctcgt
     1921 cgacggtggt gatcgacacc atgtcgccgg cggctgccag cgtggcgtag gtcagcgttc
     1981 ccgcccgccg caccgcggcg ccgtcgacga actggtcgac aaagtcaagc gtcaccggct
     2041 caccggcggc cagcgcggcc atcatcgccg cagcgccggc cggctcgatg cccaacaccg
     2101 ccgtgctcgt cgtccgctca gccaggtagg tggtgatgcc cgcgatgcag cctccaccgc
     2161 cgaccgggac caccaccagg tcgggctcgg caccgagctg atcaagcact tccacggcga
     2221 tggtgccctg ccccgccatg gtgcgcaggt cgtcgtacgg cggcaccagc gtggccccgg
     2281 tgcgtttaac gtcctcgagg gccgcgtcgg cggcgaagtc gtaggtcgat ccccccacga
     2341 tcagctcgat gaactccccg ccgtggtagc gaatgcggtc gcgcttttgt ttgggcgtct
     2401 tggcgggcac gtacacgcgg ccccgcactc ccagcttccg gcacgcatac gcgaaaccct
     2461 gcgcgtggtt gcccgccgac gagcacacca cgccggcggc catctcctcg tcggtcagct
     2521 gcaccaaaag gttgtaggca ccgcgcagct tgtaggagcg caccgtctgc agatcctcgc
     2581 gcttgaggta gacctgtgcg ccggtgatcg ccgatagccg atcgctgaac tgcagcgggg
     2641 tcggcgtgac caccggggcg atccgccgcg ccgcgctgtc gatatcggcc gcgcacagcg
     2701 gcgacatgct ggggcttctg ctcagttcag cggacaccgg tcaatggtgc catgtcactc
     2761 aaagggcgaa ttcagccctg ctggaatcag ctgactgggg tcagcacgaa cagcgggatc
     2821 tggcggtcgg tctttttctg gtatccggcg tagtcgggcc agacctcgac ggcacgctcc
     2881 caccaggcgg ccttctcgtc gccgaacacc tcgcgggcgt cgtaatcacg cgtgacggta
     2941 ccgtcctgca actcgacccg cgggttcgcc acgacgttgt agtaccagac cgggttcttc
     3001 ggcgccccgc tgagtgaggc gacgatcgca tactcgccgt cgtgctcgac ccgcatcagc
     3061 ggggtcctac ggagtttgcc cgatttggct ccgacggtgg tcagcaggat gacgggcttt
     3121 ccctgcatgt cggtgcctac ggttccgccg gattcgacgt acttgtcggc ttgctcacgg
     3181 gcccaatcga ctgggctggg tgcgtattca cctgaaagcg gcataccgcc caccttaggc
     3241 cgacggggtc gcgggtcgcc gtcggaccgg tcgtcaagac tccgacgcaa ccccggactt
     3301 ggcggcgggc ctggccagct cggcaatcgt gttgacccgc cggacggccg tggcgatctc
     3361 gtcggcgaat tggcgtcgcc gttcggcaat ttccgggtcg tcgaagccgt ccaccagttc
     3421 acggtgccgg gccagtcgta gcgcggtctt gaacaactcc atcgacctcg actccgcgct
     3481 ggcgatcctg cgctgcagct cccactgctt gcccacctca aggcactcgg tgagaaatgc
     3541 cttctcgtcg aacgatccgg actcgcgggc ggccagccgg tcggcgacga tgtggtaggc
     3601 gtccaggaac ggacgcaaca ccagatgcgc cagaagcagg tcggcgtctt ccaatagccg
     3661 acgcacgtag gccgcgctga cgggcttggt gatgtcctcc accgggccga tcagccgcac
     3721 ttcatcggcg aggtcctttt cgaattgcgc gcgtgcggag aacaagaact cgaacttcag
     3781 cagctcgcgc aggttcaacg cctcgtcgcg caccgtcccc ggttgaaccg agccctccat
     3841 ggtggtttca gcgttttccg ccgcggccag cagtgcggtc tcggcaatgg cgcgatcgac
     3901 caggatgtgg atggccgtgt tgcggtagaa cgccgcgacc aggtgttggt ccgcgccgat
     3961 gccccagacc gcttcggtgc cggcgtcgta gacgctgatc acgccggagg cgacgagctc
     4021 atgcagagtc caccggattg tcgagcgatt ggtcaggtcc gcggccccgg ccaccgccca
     4081 gttccgcgcg gcgatgtagc tcgccagtgg tcgcacggtg gccagcacct cgctgatgga
     4141 caacgaccgg tccgcaccga gcaacgcgag gttcaccacc gcaatcggtg tgaccggcgt
     4201 tgcgcggttg atccggtgct cgacgtccag cgcgatgcgc tcgatttcgg tgccggtccc
     4261 ggattcctcg gcacgcagtt cctcgaggcg tttgcgcagc ggcagcggct caccgaagtc
     4321 gacgtaggcg cgacccagtc gctcgccctg ctgtcgcgcc agccggatca ggaagcgaag
     4381 gtcctcgggc cgcttcgtgg caccgtaggc ttccgtcgtc atggcctcca cctcgtgcag
     4441 ctggtcgtag acgatggagg tgggcaccaa atacacttcg ggaccatcga tttcgtcgac
     4501 ggcgtcggtg atgtaccgca ggatcccgta caccggaggc cgtaacttgc cggtccgggt
     4561 ccggccccct tcgatcgacc aggtgaggtt gtcgtggttt tgcaccagct gcgcgacgta
     4621 ggcgcgtaac acgaaccggt agacgggaat atctttggtg tcacgccgga tgaagatcgt
     4681 tccggtgcgc ttggcccaaa cccccatcgg gaagaagttc aagttggctc cgccgaaggt
     4741 gagcgcgggc gagagccggt tggcctggat cacctccggc agcagcatgc cgtccagata
     4801 ggagcggtgc gaaaaggcaa aagccagagt cgctttgcga tccaatttgc gcagttgggc
     4861 gatctgatca tcgtcgacca gcacgtcgta ggcccgcatg aaccagcgac tgaagccacg
     4921 ccaggcccgc accgcccggt cgtccagctc ggcggacatc tcgcgcagat agcctgccgc
     4981 ctccgcgcgc acgctgtccg gatcccgctc gagctcgttg gccaactcga caagccgttc
     5041 gtcgtaccac ggcgactcca gcagctggac aacctcgacc gggtcggtcg gcaacggcgc
     5101 cgtcgattca tcgataactc cggtgcgctg caacaccttt cgcaggccta ctcgaccgat
     5161 ctcgcgcacg gtcaccttgc tcccccgctc acgccggccg cgctgctgcg gctccgggac
     5221 gttctgttgc ggtcatttcc gtcctcctca gcggcggatc agttctgcgc acacatcggt
     5281 gagcgtacgt ccgcgattat ggacgcggaa tacgtagcgt cattcagccc ccacaggcgc
     5341 gatagcgctc ggcgagggcg cgatacgagg cctcgtcacc gcgagcccgc gcgagcaacg
     5401 cctgcagccg atcgccgcct ggtgcacatc gccctggcat tggtctacaa cacccgcttg
     5461 aacggccata gctggcctcg atcggcgaat tcctgacgct aaatcgaaag tggtcgatag
     5521 cgcaacagtg gcttacgccg tcttttgcat ctattggtgc ttacggcgac aaaacaaaaa
     5581 tgcaggcggc agccctaaca gttgcgaatt ctgcaatcag atattcgcat gggtcgtaac
     5641 cggctacgca tcctagacgg ccggcgacct gttaagtcgc gtagtccact actcgggtgc
     5701 agtggcgtcg gagctgacac gctgttgccg ccagtacgca cctgagaggg agaacgacga
     5761 tggaaatcac gatcgaacga tgcggtggat tgataggtca agcagaaagg ctcgggcccg
     5821 tcgatactgc ggcgctgcct caagatgtcg cccgcaaaat cgacgccatc atctccgaga
     5881 tgaagttctt cgacctccct gtcaccatca ctaagcctgg cggagccgac ctcgtcgagt
     5941 acagaacgat cgttgccgac ggtagccgaa cccacaccgt ttactcgaac gaactgtcag
     6001 acgcggcata tcaaatggaa cttggcgatc tgatcggcct gctcgaggcg gcgggattca
     6061 agttcgtagt gatagagctc ggaatcacat cgaagatcgc gatggaagca tcgcgatacg
     6121 acctcaccgg tggcggcata acgctcgagt actttacgtt cgccaacctc aacaaagagc
     6181 ggatcctgtg gtacacggac ggccgccgca cactggagtt ctacgaaaac gacgtccgca
     6241 ccgccgac
//
LOCUS       DASSGQ010000165         5350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1348970,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000165 DASSGQ010000000
VERSION     DASSGQ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348970"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VFI55_07390"
     CDS             <1..138
                     /locus_tag="VFI55_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016719164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET7073889.1"
                     /translation="RADARPHEIHVVTSDKELTNRVANLGASVYPAASFRDLIDPRGR
                     G"
     gene            complement(291..1214)
                     /locus_tag="VFI55_07395"
                     /pseudo
     CDS             complement(291..1214)
                     /locus_tag="VFI55_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
     gene            1566..2231
                     /locus_tag="VFI55_07400"
     CDS             1566..2231
                     /locus_tag="VFI55_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET7073890.1"
                     /translation="MTPESISTQTRTYRSELRQQQAEQTRSRVVAAAAQLFAADGYAR
                     TTLAKIAAAAGVSTETVQGQGPKAALLIAAAEFAGVGVSGEENILNLDVGRKLIAIED
                     PHEALDFAVAFVTDVHKGTAKLAPALFGGANSDPELDRYLNEFFAGISGQVRRVLDVF
                     RDRGWLRCDVPFDELVETGVVLCSVETYLRITHRDGWSTEAYQAWCRRMLAETVFRTP
                     QAN"
     gene            complement(2331..2825)
                     /gene="tpx"
                     /locus_tag="VFI55_07405"
     CDS             complement(2331..2825)
                     /gene="tpx"
                     /locus_tag="VFI55_07405"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HET7073891.1"
                     /translation="MAQITLRGNPINTVGELPAVGSPAPAFNLTGADLGALSSEQFSG
                     KPVLLNIFPSVDTPVCATSVRTFNERAAGSGAAVVCVSKDLPFAQKRFCGTEGIENVT
                     TASAFRDSFGEDYGVSIVDGPMAGLLARAVVVIGPDGNVAYAQLVPEIAQEPDYDAAL
                     AALG"
     gene            2936..3886
                     /locus_tag="VFI55_07410"
     CDS             2936..3886
                     /locus_tag="VFI55_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HET7073892.1"
                     /translation="MTRKVVIVGFPGVQALDVVGPHDVFTGASLLTGGGYDVVMASVD
                     GQPVTTATGLAFLAKPLPQPGDQIDTVVLPGGGGVDAARCDPELLAWITRVAATARRI
                     VTVCTGAFLAAEAGLLDGRRVTTHWAFAGRLAREFPTVDVDPDPIFVRSSDTVWTAAG
                     VTAGIDLALSLVEDDHGTEVAQTVARWLVLYLRRPGGQTQFAAPVWMPRAKRTSIREV
                     QEVIEAQPGGPHSIGELARRAAMSPRHFTRVFTDEVGEAPGHYVERIRTEAARRQLQE
                     TDDTVVAIAARCGFGTAETMRRNFIRRVGISPDQYRKAFA"
     gene            3914..4648
                     /locus_tag="VFI55_07415"
     CDS             3914..4648
                     /locus_tag="VFI55_07415"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET7073893.1"
                     /translation="MTQIAFVAYPGFTALDMIGPYEVLRNLPGAEVRFVWHETGPITA
                     DSGVLVVGATHSLAETPSPDVILVPGGPSTPVHARDEALLDWLRRAHRTASWTTSVCS
                     GSVILAAAGLLEGRRATSHWLVVPMLKAFGAVPVADERIVRQGDIVTAAGVSAGLDLA
                     LWLAGQLGGEGRAKAIQLAIEYDPQPPYDSGHLSKASATTKAAATALLSRDSVKPAHL
                     KASTMLAWQQALAAVRSRRRGRVPVG"
     gene            complement(4668..5144)
                     /locus_tag="VFI55_07420"
     CDS             complement(4668..5144)
                     /locus_tag="VFI55_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7073894.1"
                     /translation="MGKFSRAEIEQALGNYTAVVEGCSGSGDWRPFADLFTEDVVYTE
                     HHYGVFHGREAVRDWIVAVMKPFPHMRFPNDWVAYDEDNDAVVVLVKNLLDHPTDPNG
                     EPFWFPNWTRLVYAGDGLFSSEEDIYNPNRDAPRVIAAWMQAGGELASTEILAPKT"
     gene            5204..>5350
                     /locus_tag="VFI55_07425"
     CDS             5204..>5350
                     /locus_tag="VFI55_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7073895.1"
                     /translation="MNVLGLFDLHGKRALVTGASSGIGKKVALAYVQAGAEVAIAARH
                     SDVLD"
BASE COUNT          883 a         1752 c         1820 g          895 t
ORIGIN      
        1 cgagccgacg cccggccgca cgaaattcat gtggtgacat cggacaagga actaaccaac
       61 cgcgtcgcaa atttgggcgc ctccgtttac cccgcggcaa gtttccgtga cctgatcgac
      121 ccgcgcggcc gaggctgaca aggtcgacgc gtcatcgacg acctcccgcc cggcggccga
      181 catcatcacg cggatgtgga ccgggtgccg agaggtgccg gccgccacga cggatcgact
      241 tgggccgcgg acaaccgatc aacgacgtcc gctaggcccg cgaagcctgg ggcggccgcc
      301 atgtcggccg cgatgcgccg gctggcttgg cggtagcgcg gctgattcag caccgcgaaa
      361 atctcgcggc gcagcgcacg tggtgtcggt cgttcggttc tgatgcgccg cccgacgccc
      421 gaccatgcga tgcgagcgcc tacctccggt ttgtcttctt taccgcctgt ggcgacgatc
      481 ggcacgccgt agcgtagcgc gcactgcacg ccgccgtagc cgctgttggt cacgtaaacc
      541 gatgtgcgtg gcagtaattc gtcgtatggc agatacatgg ccgcgcgtgc gttcggcggc
      601 aacgggggca gcgcgtccag cggccggccg cccgttgcga ccgcgaccag gacgtcgtcc
      661 tcggcgagcg cacgcagcgt cggcgcgatg acctggtcgt agtcggtgtt cgcgaccgtg
      721 ccctgagtga catggacgat gggccgcgtc ccgtcaagat cgccccacca ctccggcaac
      781 ggtgtctcga agccgctggc cgacaatggt ccgatgtagt gcaggttggc gggtacatcg
      841 gacctggggt actcaaatga gggaacgctg aactgcacga tggcgtcggc acggcggccc
      901 caatcggcca tggttcctgg catggctttg ccgtgcacct cttcgtgcat gtcgtcgaag
      961 agttggtagg cccgtcctaa cgctcgacgg ctcactgcag ccagaacggc attgcgctga
     1021 cgattgagca cgcgggccgg ccgcaacccc atgccgtagg gtgcggtgtc gcggctgtcg
     1081 atgaaaagcg ggatcaccga gcacatgacg accgcggggc gcgttgaacg tgggtgctcg
     1141 agcatgaacg tagcgccgaa aaagcaaggc tcagcgagca cgacgtcggc ggactgtgcc
     1201 gcaacggccg tcatgagcgt ctcgtactgg gccttggcgg ggcgcgcaaa gacattctcg
     1261 acatcgaagg cgatcgcatt gatgcccttg aggtttacgc gttcgggaaa gctctcgaat
     1321 aaattctggt cgtcgaagtc ggcctcggcg ggcaacggca caaaggtcgc tcccgtggcc
     1381 gcgaccttgt cggcgtaacg cgagccggtc acgaaccgta cgtaatcgcc gcgcttgacg
     1441 aagttttcgg ccaccgtcag cagcggcgcg acgtgcccgt ggacggggat gctggcaacg
     1501 atgatggatg acactgctgc acccccggga tcgccgttag ctaagtaatg gtagtattca
     1561 ttactgtgac accggagtca atatcgacac agacgcgaac ctaccggtcc gagctgcgtc
     1621 agcagcaggc ggagcagacg cggtcacggg tggtcgcggc cgccgcgcag ctgttcgccg
     1681 ccgacggtta cgcgcgcacc acgttggcga aaatagcggc tgcggccggt gtttcgaccg
     1741 agacggtcca gggccagggg cccaaggctg ccctcttgat cgccgccgcc gagttcgccg
     1801 gagttggcgt gagtggcgaa gaaaacattc tcaacctgga tgtcggccgc aaactgatcg
     1861 ccatcgagga cccgcacgaa gcgctcgact ttgctgtcgc gttcgttacg gacgtacaca
     1921 aggggaccgc gaaactggcg cccgcgctat ttggcggcgc taattccgac cctgagttgg
     1981 atcggtacct gaacgagttc ttcgccggga tcagcgggca ggtccgacga gtcctcgacg
     2041 tgtttcgcga ccgcggatgg cttcgttgcg acgtgccgtt cgacgaactc gtcgagacgg
     2101 gggtagtgct ttgcagcgtc gagacctatt tgcggataac acaccgcgac ggttggagca
     2161 cagaggctta tcaggcgtgg tgccggcgca tgctggccga gaccgttttc cgtacaccgc
     2221 aagcaaatta gctcgatcga gcgtgcgcag ttgtacgcgt gcggcggcgt gtcgtcgtac
     2281 agacacgcac gttcgcggta ggccgtaggc ggctaggcca gcagcgggcg ttagcccagc
     2341 gccgccagcg ccgcgtcgta gtcgggctcc tgcgcgattt ccggcaccag ttgcgcgtac
     2401 gcgacgttcc cgtcggggcc gatcaccacg acggcgcggg cgagcaaccc ggccatcggg
     2461 ccgtcgacga tgctgacgcc gtagtcctcg ccgaagctgt cgcggaatgc cgacgccgtc
     2521 gtgacgttct cgatgccctc cgtcccgcag aatcgcttct gggcgaacgg cagatccttc
     2581 gacacgcaca ccaccgcggc gccgcttccg gcggcgcgct cgttgaaggt ccgcacgctc
     2641 gtcgcgcata ccggggtgtc caccgacgga aagatgttca gcaacacggg cttaccgctg
     2701 aactgctcgc tgctgagagc gcccaggtcg gcgccggtca agttgaaggc tggggccgga
     2761 gatccgacgg caggaagctc gccgactgtg ttgatcgggt ttccacgcaa ggttatctgt
     2821 gccatgcgca cagtctgcca aggccggcgc ggcggcttcg ggtcaggtcc gcatgtccta
     2881 aatggtgggg tacccgacgt agacgccacg cgccggtccg gccaacacta agacggtgac
     2941 ccgcaaagtg gtgatcgtcg gctttccggg cgttcaggcg ctcgacgtgg tggggcccca
     3001 cgacgtcttc accggtgcgt cgctgctgac cggcggcgga tacgacgtcg tgatggcgtc
     3061 ggtcgacggc caaccggtca ccaccgcgac cggcctggct ttcctggcga agccgctgcc
     3121 gcaaccgggc gaccagatcg acaccgtcgt gctgcccggc ggcggtggtg tcgacgccgc
     3181 gcggtgcgac cccgagctgt tggcgtggat caccagggtc gccgcaaccg cccgccgcat
     3241 tgtcaccgtc tgcaccggtg cgtttctggc cgccgaggcg ggactgctgg acggccggcg
     3301 ggtgacgacg cactgggcgt tcgccgggcg actggcgcgc gagttcccca ccgtcgacgt
     3361 cgatcccgac ccgatcttcg tccgcagctc ggacacggtg tggacggccg ccggtgtcac
     3421 cgcgggaatc gacctcgcgc tctcgctcgt ggaggacgac cacggcaccg aggtcgcgca
     3481 gacggtcgcc cgctggctgg tgctgtattt gcgccgcccc ggcgggcaga cgcaattcgc
     3541 ggcgccggta tggatgccac gcgccaaacg gacctcgatc cgcgaagtgc aggaggtgat
     3601 cgaggcgcaa ccgggcggcc cgcacagcat cggcgagctg gcccgccggg cggcgatgag
     3661 cccgcgccat ttcacgcggg tgttcaccga cgaggtcggt gaggcgcccg gccactacgt
     3721 cgaacgcatc cgaaccgaag ccgcgcgccg gcagttgcaa gagaccgacg acaccgtggt
     3781 cgcgatcgcc gcccggtgcg gcttcgggac cgcggaaacc atgcggcgca acttcattcg
     3841 ccgcgtcggc atttcgccgg accaataccg caaggctttc gcgtaaccag agaggacgac
     3901 accgatgtcc aagatgaccc agatcgcctt cgtggcctac cccggattca ccgcgctgga
     3961 catgatcggc ccgtacgagg tgctgcgtaa cctgcccggc gccgaggtgc ggttcgtgtg
     4021 gcatgaaacc ggaccgatca ccgccgactc cggtgtgctg gtcgtcggcg ccacccactc
     4081 gctggccgaa accccttcgc ccgacgtgat tctggtgccc ggcggcccgt cgaccccggt
     4141 gcatgcacgt gacgaggcgc tgctcgactg gctgcgtcgg gcccaccgga ccgccagctg
     4201 gacgacgtcg gtgtgttcgg gctcggtgat ccttgcggcc gccggcctgc tggagggccg
     4261 gcgcgcgacg tcacactggc tggtcgtgcc gatgctgaag gcgttcgggg ccgttcccgt
     4321 cgccgacgag cgcatcgtgc gccagggtga catcgtcacc gctgcgggcg tgtccgccgg
     4381 cctcgacctc gcgctgtggc tggccgggca gctcggcggc gaaggccgcg cgaaggcgat
     4441 tcagctcgcc atcgaatacg acccgcagcc cccctacgac tccggccacc tgtcgaaggc
     4501 atcggcgacc acgaaagccg ctgccaccgc gctgctgtcc agggacagcg tcaagccggc
     4561 gcacctgaag gcctcgacga tgctcgcgtg gcagcaggcc ctggcggcgg tccggtcgcg
     4621 ccgccgcggg cgggtgccgg ttgggtgatg actgacaccc cgaagcgcta cgtcttgggc
     4681 gccaggatct cggtcgaggc gagctcgccg ccggcctgca tccacgcggc gatcacacgg
     4741 ggcgcgtcgc ggttcgggtt gtagatgtct tcctcgctgg aaaacaggcc gtcaccggcg
     4801 tagacgagcc gggtccagtt cgggaaccag aacggctctc cattcgggtc ggtcgggtgg
     4861 tcaagcaggt tcttgaccag cacgacgacg gcgtcgttgt cctcgtcgta ggcaacccag
     4921 tcgttgggaa agcgcatgtg cgggaacggt ttcatcaccg ccacaatcca gtcccggacc
     4981 gcctcccgcc cgtgaaagac gccgtagtga tgctcggtgt agaccacatc ctcggtgaaa
     5041 aggtcggcaa aagggcgcca gtcgcccgac ccgctgcacc cctccaccac ggccgtgtag
     5101 ttccccaagg cttgctcgat ttcggccctg ctgaatttgc ccatagcgga cacactagaa
     5161 cgtgtttcgg tttacggatc agtcgtctgg atggggttag cgcatgaacg tgttgggctt
     5221 gttcgatttg catggcaaga gggctctggt gaccggggcg tccagtggta tcggcaagaa
     5281 agttgcgctg gcctacgtcc aggcaggcgc cgaagtggcc atcgcggcac ggcattccga
     5341 cgtcttggac
//
LOCUS       DASSGQ010000166         3803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_377032,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000166 DASSGQ010000000
VERSION     DASSGQ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377032"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..97)
                     /locus_tag="VFI55_07430"
     CDS             complement(<1..97)
                     /locus_tag="VFI55_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073896.1"
                     /translation="MKLARPDVFHPRLVLAGCRGHVAGDGDDAGLL"
     gene            117..1091
                     /locus_tag="VFI55_07435"
     CDS             117..1091
                     /locus_tag="VFI55_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HET7073897.1"
                     /translation="MFAIQIIETGGPEVLRYVDTAQPVPGPGEVLIKAEAIGVNYIDT
                     YFRTGQYPRELPFIIGSEMAGKVVSTGEGVDGFRAGDRVVTATASGGYAEFSTAPAAL
                     TAKVPDNVTSDVAASVLLKGLTAHYLLKSVYPVQSGDAVLVHAGAGGVGLILTQWAAL
                     LGVRVITTVSTPDKARMSKEAGAAEVLSYPDDALEFGQQVRALTGGAGVAAVYDGVGA
                     TTFDASLASLAVRGTLALFGAASGPVPPLDPQRLNAAGSVFLTRPSLAHFIRTGDEFT
                     WRATELFDAISSGAITIEVGGRYPLAEAARAHLDLQGRKTAGSIVLLP"
     gene            complement(1250..2176)
                     /locus_tag="VFI55_07440"
     CDS             complement(1250..2176)
                     /locus_tag="VFI55_07440"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015289869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HET7073898.1"
                     /translation="MSVRGRVAPSGVPSRVRGTVLAYLALTKPRVIELLLVTAIPAML
                     LAHRGTVNPLLILNTLVGGMLAAGGANTLNCVADADIDKVMKRTARRPLARAAVPTRN
                     ALVLGVLLSVGSFFWLWWTTNLLSGLLAIATIAFYVFVYTLLLKRRTSQNVVWGGAAG
                     CMPVMIGWSAVTGTIGWPALVMFAIIFFWTPPHTWALAMRYKDDYKRAGVPMLPAVAT
                     EREVTKQILIYTWLTVVATLALALATGWLYAAVALLAGVWFLTMAHQLYAGVRAGEPV
                     KPLRLFLQSNNYLAVVFCALAVDSVIALPTLL"
     gene            2434..>3803
                     /locus_tag="VFI55_07445"
     CDS             2434..>3803
                     /locus_tag="VFI55_07445"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7073899.1"
                     /translation="MTTLEEISTLTQPHHPDDWAEIDSAAVDTVRVLAADAVQKVGNG
                     HPGTAMSLAPLAYTLFQRAMRHDPNDTHWLGRDRFVLSAGHSSLTLYIQLYLGGFGLQ
                     LSDIESLRTWGSKTPGHPEFRHTRGVEITTGPLGQGLASAVGMAMASRYERGLFDPDA
                     EPGTSPFDHHIYVIASDGDIEEGVTSEASSLAAVQQLGNLIVFYDHNQISIEDDTNIA
                     LCEDTAARYRAYGWHVQEVEGGENVVGIEEAIANAKAATDRPSFIALRTIIGYPAPKL
                     MNTGKAHGAALGDEEVAAVKRILGFDPDKTFEVREDVITHTRGLVARGKEAHESWQAD
                     FEAWAQREPERKALLDRLLAQQLPDGWDADIPHWEPGSKALATRAASGEVLSAVGPKL
                     PELWGGSADLAGSNNTTMKGVDSFGPPSISTSDYNAHWYGRTLHFGIREHAMGAILSG
                     IVLHGPT"
BASE COUNT          689 a         1323 c         1224 g          567 t
ORIGIN      
        1 ccagcagccc ggcgtcgtct ccgtcaccgg cgacatgccc tcgacaaccc gccaacacga
       61 ggcggggatg gaagacgtcc ggccgggcga gtttcatgca cgggcatgat aaccacatgt
      121 tcgcaatcca gatcatcgaa accggcggcc ccgaggtact gcgctacgtc gacacagcac
      181 aacccgtacc gggcccgggc gaggtgctga tcaaggccga ggccatcggc gtcaactaca
      241 tcgacaccta cttccgcacc ggccagtacc cccgagagtt gccgttcatc atcggctccg
      301 agatggccgg caaggtcgta tccaccggcg agggggtcga cggattccgc gcgggcgacc
      361 gggtggtgac cgccaccgcg tccggcggct acgccgaatt ctccactgcc ccagcggctt
      421 tgaccgcaaa ggtgccagac aatgtgacgt cggatgtggc ggcttcggtg ctgttgaagg
      481 gcctgaccgc gcattatctg ctcaagtcgg tctatccggt gcagagcggc gacgcggtcc
      541 tggtgcatgc cggcgccggc ggcgtcggac tgatcctgac gcagtgggcc gcccttctgg
      601 gcgtccgggt catcaccacc gtgtccaccc cggacaaggc acggatgtcg aaggaggcgg
      661 gcgccgccga ggtgctctcc taccccgacg acgctctcga gttcgggcag caggttcgcg
      721 cgttgaccgg gggcgccggg gtggccgcgg tgtacgacgg cgtgggcgcg accacctttg
      781 acgccagcct ggccagcctc gccgtgcgcg gcaccctggc actgttcggc gccgccagcg
      841 gacccgttcc gccccttgac ccgcagcgcc tcaacgccgc gggctcggtg ttcctgacca
      901 ggccctcgtt ggcgcacttc atccgtaccg gcgacgagtt cacttggcgg gcaaccgaac
      961 tgttcgacgc gatctccagc ggcgccatca cgatcgaagt cgggggtcgc tatccgctcg
     1021 ccgaagcggc tcgggcccac ctggacctgc agggccgcaa gacggccggc tcgatcgtgc
     1081 tgctgccgta gatccgtcac ggcagccaca gcagtctctg cggggtgggc acccgacttt
     1141 ttgcccacct cacagcgact tgctcgcccg ccgttcgaag tcgctgtgag gtgggcaaaa
     1201 aatgtgccgc ctcccggaga gacgcacggg gcaaatgtga cttgacgcct cagagcagcg
     1261 tcggcaacgc gatgaccgag tcgaccgcca acgcgcagaa cactaccgcc aggtagttgt
     1321 tcgactgcag gaacagtcgc agcggcttga ccggctcgcc ggcgcgcacc ccggcataga
     1381 gctgatgcgc catcgtcaag aaccacaccc cggccagcag ggcgacggcc gcataaagcc
     1441 accccgtcgc cagcgccagc gccagcgtcg ccaccacggt caaccaggtg tagatcagga
     1501 tctgcttggt gacctcgcgc tcggtcgcca ccgcgggcag catcgggacg cccgcccgtt
     1561 tgtagtcgtc cttgtagcgc atcgcgagcg cccaggtgtg cggcggcgtc cagaagaaga
     1621 tgatcgcgaa catcaccaac gccggccagc cgatcgtgcc ggtgacggcc gaccagccga
     1681 tcatcaccgg catgcagccg gccgcaccgc cccacacgac gttttgcgac gtgcggcgtt
     1741 tgagcaacag cgtgtaaacg aacacgtaaa acgcgatggt ggcgatggcc agcaggcccg
     1801 acagcaggtt ggtcgtccac cacaaccaga agaacgagcc cacgctgagc agcactccca
     1861 gcaccagggc gttacgggtc gggaccgcgg cccgtgccag cggccggcgc gcggtccgct
     1921 tcatcacctt gtcgatgtcg gcgtcggcca cgcagttgag cgtgttggca ccgcccgcgg
     1981 ccagcatccc gccgaccaac gtgttcagga tcagcaacgg attcacggtg ccccgatggg
     2041 cgagcagcat cgcgggtatc gcggtgacca gcagcagctc aatgacccgc ggcttggtca
     2101 acgccagata agccagcacc gtgccacgca ctcggcttgg tactccgctc ggcgcgacgc
     2161 gcccgcgaac gctcacgcat taactcctcg gggtcgcagc atcgcgcatc atctactacg
     2221 cacgatggta gaccgcgtac cggcggtggc cagcccgcag ggtagattcc cgcagatttt
     2281 tgccgcgtca cgggaccgat tgcaaagcgt gagcagccaa cgcgtaattt ttgacagcgg
     2341 ccggccgcgg gtacccgaaa ttctgcgctg caagagccgg cactcccgca ttagggtgag
     2401 caatatcagc tcgatgaccc aaggactgag gctgtgacca cactcgaaga gatctccact
     2461 ctgactcaac cccaccaccc tgacgactgg gccgagatcg attcggctgc tgtcgacacc
     2521 gtccgggtgc tggcggccga cgcggtgcaa aaggtcggca acgggcaccc tgggacggcg
     2581 atgagcctgg ccccgctggc gtacacgctg tttcagcgcg cgatgcgcca cgaccccaat
     2641 gacacgcact ggctgggccg tgacaggttc gtgctgtcag ccgggcacag cagcctgacc
     2701 ctgtacatac agctctacct cggcggcttc gggctgcagc tgtccgacat cgaatcgctg
     2761 cgcacctggg gatccaagac tcccggacac ccggagttcc ggcacaccag gggcgtcgag
     2821 atcaccaccg ggcctctcgg tcagggcctg gcatccgcgg ttgggatggc gatggcgtca
     2881 cgctacgagc gcggcctgtt cgatcccgac gccgaaccgg gaaccagtcc cttcgaccac
     2941 cacatctacg tgatcgcctc cgacggggac atcgaagaag gcgtgacgtc cgaggcgtcg
     3001 tcgctggcgg ccgtgcagca gctgggcaat ctcatcgtgt tctacgacca caaccagatc
     3061 tcgatcgagg acgacaccaa catcgcgctg tgtgaggaca ccgccgcgcg ctaccgcgcc
     3121 tacggctggc acgtccaaga ggtcgagggc ggcgagaacg tcgtcggcat cgaggaggcc
     3181 atcgccaacg cgaaagccgc caccgaccgg ccgtcgttca tcgcgctgcg caccatcatc
     3241 ggctacccgg cacccaagtt gatgaacacc ggcaaggcgc acggcgcggc cctgggcgac
     3301 gaggaggtcg ccgccgtcaa gcgcatcctc gggttcgatc ccgacaagac gttcgaggtg
     3361 cgcgaggacg tcatcaccca cacccgggga ctggtggccc gcggcaagga ggcccacgag
     3421 agctggcaag ccgacttcga ggcctgggcg caacgcgaac ccgagcgcaa agccctgctg
     3481 gaccggctgc tcgcgcagca gctgcccgac ggctgggacg cggacattcc gcactgggaa
     3541 cccgggtcca aggcgctggc cacccgcgcg gcgtccggtg aggtgctgtc ggcggtgggt
     3601 ccgaaactgc ccgagttgtg gggcggttcg gccgacctgg cgggcagcaa caacaccacc
     3661 atgaagggcg tggattcctt tggtccaccg tccatttcga ccagcgacta caacgcgcac
     3721 tggtacggcc gcacgctgca cttcgggatt cgcgaacacg cgatgggcgc catcctgtcc
     3781 ggcatcgtgc tgcacggccc gac
//
LOCUS       DASSGQ010000167         9231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1470262,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000167 DASSGQ010000000
VERSION     DASSGQ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9231
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470262"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..1248
                     /locus_tag="VFI55_07450"
     CDS             13..1248
                     /locus_tag="VFI55_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7073900.1"
                     /translation="MNTPWMLRGVDLAAFAAALVARLRGVGVLVSANGQATFVQALRL
                     LVPDTTVALYWAARLTLVNRMEDLSAFDAVFTSVFGPVDTTSPDGATGRGPVLPLPGP
                     KTPAAGTLRRPGGRSAATQTLPWATRITTQRDDRTEPGRLVFDVLPSRIAALADEPFD
                     RFDPEDLRVLGSWLEASVQRWPRRRSLRFEPSAGGKRIDLRATMNASRATGWESVILA
                     RTRPRRRPRRVVLVCDLSRSMQPYAAIYLHLMRAAALRQARIHPEVFAFSTSLTRLTS
                     VLSHRSAEVALQRANARVTDRYGGTFIGRSVGALLTPPHGNALRGAVVVIASDGWDSD
                     PPEVLGHAMARLRRRAELLVWLNPRAAQSEFQPLAGSMAAALPYCDLFLPAHSLSGLY
                     DLFLALASGESALTALSAR"
     gene            1343..2482
                     /locus_tag="VFI55_07455"
     CDS             1343..2482
                     /locus_tag="VFI55_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 76 protein"
                     /protein_id="HET7073901.1"
                     /translation="MDQLWVNRAASSEAAVAQRHLKRLWLLPGTQLGVVAWPAARRDR
                     MFGTWHYWWQAHLLDCLVDAQLRDPQPDRHTKINQQVRSHRIRNNFSWTNNYYDDMAW
                     LALALERAGRIAGVERRRALPKLCDQFVKAWVPEDGGGIPWRKEDQFFNAPANGPAGI
                     FLARYSDSYGDRLKRAGQMADWIDRTLIDPETHLVFDGIKAGSLVRAQYTYCQGVVLG
                     LETELAARTGAEVRARHAARVHRLVAAVNEHMAPSGVLQGSGGGDGGLFAGVTARYLA
                     LVATTLPGDSADDAVARDTARAVVLASAKSAWDYRQTVNGLPLFGAFWDREAELPTTG
                     GPEAELVGGAVFPSAVAERDLSVQLSGWMLMEAACSVTALTPGDR"
     gene            2509..3858
                     /locus_tag="VFI55_07460"
     CDS             2509..3858
                     /locus_tag="VFI55_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HET7073902.1"
                     /translation="MTVSKVHPDAESALRGLLRDGITIAAGGFGLCGIPENLIQALVD
                     SGVKDLTIVGNNAGVDDFGMGLLLRGRQVKKVIASYVGENKEFERQVLAGELDLALTP
                     QGTLAEKLRAGGAGIPGFYTRTAFGTQLADGKDIRVFDGTEYVLEEGIRADVAIVKAW
                     KGDTAGNLVYRETARNFNPMIATCGNVTVAEVEELVEVGELDPDLIHTPGIYVDRIIE
                     GPGYEKRIEFRTTRGAAVKNDSPIRELMAKRAARELRDGYYVNLGIGIPTSVANFIPD
                     DINVTLQSENGLLGIGAYPADDQVDPDLINAGKQTITTIRGSSFFNSADSFAMIRGGH
                     IDLSILGGLEVAENGDLANWMVPGKMVKGPGGAMDLVSGVKRVIVVMDHTARDGSPKI
                     LKECTLPITGKGVVDMIITDLCVFDVDPQRGLILTELHPGVTVADVRGNTGCDFIVA"
     gene            complement(3878..4555)
                     /locus_tag="VFI55_07465"
     CDS             complement(3878..4555)
                     /locus_tag="VFI55_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014943039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET7073903.1"
                     /translation="MSTAVMASGSILDPMFWIGPQGLFAAAVLPTILVIVFVETGLLF
                     PLLPGESLLFTGGLLAAHGTLNIWVLAPSVALVAILGDQTGYFIGRRIGPALFKKEDS
                     RFFKKHYVTESHAFFEKYGPWAIILARFMPFVRTFTPVVAGVSYMRYPVYLAFDIVGG
                     VLWGGGVTLAGYFLGNIPFVHQNLEKIILGILFVSLLPAFIATWRGFQGRRRAAKEGP
                     ERMPVPD"
     gene            4652..5683
                     /gene="fbaA"
                     /locus_tag="VFI55_07470"
     CDS             4652..5683
                     /gene="fbaA"
                     /locus_tag="VFI55_07470"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HET7073904.1"
                     /translation="MPIATPEVYAEMLGRAKENSYAFPAINCTSSETVNAAIKGFADA
                     GSDGIIQFSTGGAEFASGLGVKDMVAGAVALAKFTRSIAARYPINVALHTDHCPKDKL
                     DSYVRPLLAISAERVAAGKNPLFQSHMWDGSAVPIDENLAIAKELLKTAAAAKIILEV
                     EIGVVGGEEDGVEGAIDDKLYTTPEDFEKTIDALGHGEHGTYLLAATFGNVHGVYKPG
                     NVKLRPDILDQGQKVAAAKLGLPDGAKPFDFVFHGGSGSLKSEIEEALRYGVVKMNVD
                     TDTQYAFTRPLAGHMFANYDGVLKVDGEVGNKKVYDPRSYLKKAEAGMTERVIEACND
                     LHCAGKSIA"
     gene            complement(5796..6656)
                     /locus_tag="VFI55_07475"
     CDS             complement(5796..6656)
                     /locus_tag="VFI55_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073905.1"
                     /translation="MPNAPEPDRGGTPKRPGFDPRESWGEPSGQRAAEPHDEETEDQP
                     LVPRDTETETVVINRTDPDNDPEADPEGQRERRFTAPGFDAKETTVITTSSEPATEVL
                     PSPPGPEGATAQLGAVPKAAVPQSIPPRPGAKPPTSRQFNWGWVLALVLIVLALAAIA
                     ILGTVLLTRSKHAKTSEEDRVRHTIHEYDVAVQRGDLTALRGMTCGTARDGYVDYDEN
                     AWQETYQRVAAAKQYPVIASIDQVVVNGQHAEANITTFMAYDPSLRSTRGIDLQYRDD
                     QWKICQSPSG"
     gene            6704..7117
                     /locus_tag="VFI55_07480"
     CDS             6704..7117
                     /locus_tag="VFI55_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 domain-containing protein"
                     /protein_id="HET7073906.1"
                     /translation="MTSMGDLLGPKPILLPGDSDAEAQLRANEHPSIVAAAHPSASAA
                     WAALAEEALTEDKAVTAYAYARTGYHRGLDQLRRNGWKGFGPVPYAHEPNRGFLRCVA
                     ALARAADAIGETPEYQRCLDLLDDCDPTARKALGL"
     gene            complement(7114..8013)
                     /locus_tag="VFI55_07485"
     CDS             complement(7114..8013)
                     /locus_tag="VFI55_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET7073907.1"
                     /translation="MGAGHNHTPAETDGSRMVPRMIIAAVILATFFVVELATSLSINS
                     MALLADAGHMLTDVVAVFMGLGAVVLAQRGSSSPSRTYGWHRAEVFTAVANAALLIGV
                     AVFIFYEAVQRLSEAPSVPGMPMIVVALAGLAANFVVALLLRSHSSQSLAVKGAYMEV
                     IADTVGSLGVLIAGVVTVTTHWPYADVVVAVLVALWVLPRAISLARDALRILSESSPA
                     HIDVDELRSALGAVDGVTEVHDLHVWTLSPGKDMCTAHLTSAADSARVLHDARAVLSA
                     RGLDHATVQIECPDGTGHCEEAC"
     gene            complement(8053..8829)
                     /locus_tag="VFI55_07490"
     CDS             complement(8053..8829)
                     /locus_tag="VFI55_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET7073908.1"
                     /translation="MSFPARKRESVRPSPIFLALIGLTVVGGVLAWLAGSSVRPLSYV
                     GVFIFVLSGWLVSLCLHEFGHAFTAWRFGDHDVAVRGYLTLDPRRYSHPALSLLMPVV
                     VILLGGIGLPGAAVYLRTWFMTPTRRTLVSLAGPAANLLLAVLLLVLTRLFYDPNHAV
                     LWAGVAFQAFLQITAVLLNLLPIPGLDGYDALEPHLSPETQRALAPAKQYGFFILLFL
                     LLAASPWIFGLVYWVFDLSGVHHWLPAAGNSLTRFWSRWF"
     gene            complement(8839..>9231)
                     /locus_tag="VFI55_07495"
     CDS             complement(8839..>9231)
                     /locus_tag="VFI55_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50"
                     /protein_id="HET7073909.1"
                     /translation="VPAARRAARRARRGHARRVPLIRDETGSVIVGRADWLPAADRQL
                     VHGEAVVDDTPLFDGEVGGVRIEPTSAAPGLRASVYGSRIGRRWIAGRAAQLGSTGVT
                     VVRDGVPAPRAARRSTFYRNVEGWLLVR"
BASE COUNT         1619 a         3095 c         3115 g         1402 t
ORIGIN      
        1 cgttagcgct tcgtgaatac gccatggatg ttgcggggcg tcgacttggc ggcgttcgcg
       61 gccgccctgg tggcgcggct gcgcggcgtt ggggtgctgg tgtccgccaa tggccaggcc
      121 actttcgtcc aggcgctgcg actgctggtg cccgacacca cggtggcgct gtattgggcg
      181 gccaggctga ccctggtcaa ccggatggaa gatctgagcg ccttcgacgc cgtcttcacc
      241 tcggtgttcg ggccggttga cacgacctcg cccgacggcg caacaggccg cgggccggtg
      301 ctgccccttc cgggtcccaa aactcccgcg gcgggcacgc tgcgccggcc cggcggtcga
      361 tcggctgcca cccagaccct gccctgggcc acccggatca ccacccagcg ggacgaccgt
      421 acggaaccgg gcaggctggt gttcgacgtc ctgcccagtc gcatcgccgc actggccgac
      481 gaaccgtttg accggttcga tcccgaagac ttacgtgtgc tcggttcctg gctggaggcg
      541 tccgtgcagc gctggccccg gcgccgcagc ctgcgcttcg agcccagcgc gggcggcaag
      601 cggatcgacc tgcgggcgac gatgaacgcg tcgcgggcca ccggctggga gtcggtgatc
      661 ctggcgcgga cccgacctcg tcgccggccg cgccgcgtcg tcctggtgtg cgacctgagc
      721 cgctcgatgc agccctacgc cgcgatctat ctgcatttga tgcgggcggc ggcgctgcgc
      781 caggcgcgga tccaccccga agtcttcgcg ttctcgacgt cgctgacccg gctcacctcg
      841 gtgctgtcac accgctcggc cgaggtggcg ctccagcgag ccaacgcacg ggtcaccgac
      901 cggtacggcg gcacgttcat cggtcgcagt gtcggtgccc tgctgacccc gccgcacggc
      961 aacgcgctgc gcggcgcggt ggtcgtcatc gcttccgacg gatgggacag cgatcccccc
     1021 gaggtgctcg gccacgccat ggcccggctg cgtcggcgtg ccgagctgtt ggtctggctc
     1081 aatccacgtg cggcgcagag cgaattccag ccgctggccg gttcgatggc cgcggcgctg
     1141 ccttactgcg acctgttcct gccggcgcat tcgctgagcg gactgtacga cctgttcctc
     1201 gcgttggctt ccggcgagag tgcgctgacg gctttgagtg cgcgctgacg gcgggaactc
     1261 gaggtgaata tcctgcctct gatgcatatt cgacggtggt agacgcggat gccggtggcg
     1321 cgctaaggca ggatcgacag gtatggatca gctttgggtc aaccgggcag ccagttccga
     1381 agccgctgtg gcgcagcggc acctgaaacg gctgtggttg cttccgggca cccagttggg
     1441 agtggtggcc tggccggcgg cgcggcggga ccggatgttc gggacgtggc actactggtg
     1501 gcaggcgcat ctgctggatt gcctggtcga tgcccagctg cgcgacccgc agccggacag
     1561 gcacaccaag atcaaccagc aagtccgttc ccaccggata cgtaacaact tctcgtggac
     1621 gaataactat tacgacgaca tggcgtggct ggcgctggct ctggaacgtg ccggccggat
     1681 cgccggcgtc gagcgccgcc gtgcactgcc caagctgtgt gaccagttcg tcaaggcgtg
     1741 ggtgcccgag gacggcggcg gcatcccgtg gcgtaaggaa gaccagttct tcaacgctcc
     1801 ggccaacggc ccggcgggaa tcttcctcgc ccgctactcg gacagctatg gtgaccggct
     1861 caagcgcgcc gggcagatgg ccgactggat cgaccgcacg ttgatcgacc cggagacgca
     1921 cctggtgttc gacggcatca aggccggctc gctggttcgc gcgcagtaca cctactgcca
     1981 gggcgtggtg ctgggcctgg aaaccgagct cgccgcgcgc accggcgccg aagtcagggc
     2041 ccggcacgcg gcacgggtgc atcggctggt tgctgcggtc aacgagcaca tggcgccctc
     2101 aggtgtgttg cagggcagcg gcggcgggga cggcggcctg ttcgccggcg tcaccgcccg
     2161 gtacctcgcg ctggtcgcca ccacgcttcc gggcgactcc gccgacgatg ccgtggctcg
     2221 cgacaccgcc cgcgccgtcg tgctcgccag cgcgaaatcg gcgtgggact atcggcaaac
     2281 ggtgaacggg ctgccgctgt tcggcgcgtt ctgggaccgc gaagcggagc tgcccaccac
     2341 gggcggccca gaggcggagt tggttggggg cgcggtgttc ccttccgccg tcgccgaacg
     2401 ggatctgtcg gtgcaactgt cgggctggat gctgatggag gccgcctgta gcgtcaccgc
     2461 cctgacgcca ggcgaccggt gattggctag cgttttgagg agatttcgat gaccgtttcc
     2521 aaagtccacc ccgacgccga gtcggctttg cgaggcctgc ttagggacgg catcaccatc
     2581 gccgccggcg gattcgggct gtgcggtatc ccagagaatc tgatccaggc cctggtggac
     2641 agcggcgtta aagacctgac gatcgtcgga aacaatgccg gcgtcgacga tttcggcatg
     2701 ggtctgttgc tcagggggcg gcaggtcaaa aaggtaatcg cctcctacgt gggggagaac
     2761 aaggaattcg agcgccaggt gctggccggc gagctagacc tcgcgctcac cccccaaggc
     2821 accctggccg agaagttgcg ggccggcggc gcagggattc cgggcttcta cacgcgcacc
     2881 gccttcggca cgcaactcgc cgacggcaag gacatccggg tcttcgacgg caccgaatat
     2941 gtgctggagg agggtatccg cgccgatgtc gcgatcgtca aggcatggaa gggcgacacg
     3001 gcgggcaatc tcgtctaccg ggagacggcg cgcaacttca atccgatgat cgccacctgc
     3061 ggcaacgtaa ccgtggccga ggtcgaagag ctggtcgagg tcggcgagtt ggatcccgat
     3121 ctgatccaca cccccgggat ctacgtggac cgaatcatcg agggacccgg ctacgagaag
     3181 cggatcgaat tccgcaccac ccgcggcgcc gcggtcaaga acgacagccc catccgcgaa
     3241 ctcatggcca agcgtgctgc ccgagaactg cgcgacggct actacgtgaa cctgggtatc
     3301 gggatcccga cgtcggtggc caacttcatt cccgacgaca tcaacgtgac gctgcaatcc
     3361 gagaacggac tgctcgggat cggggcgtat ccggccgacg accaagtcga ccccgacctc
     3421 atcaacgccg gcaaacagac catcaccacg atccgcggct ccagcttctt caacagcgcc
     3481 gactccttcg cgatgatccg cggcggccat atcgaccttt ccatcctcgg cggcctggag
     3541 gtggccgaga acggcgacct ggccaactgg atggtcccgg gcaagatggt caaaggaccg
     3601 ggcggtgcga tggacctggt gtcgggggtc aagcgggtga tcgtcgtgat ggatcacacc
     3661 gccagagacg gcagcccgaa gattctcaag gaatgcacgc tgcctatcac aggaaaaggc
     3721 gtggtggaca tgattatcac cgacctgtgt gtgttcgacg tcgacccgca gcgcggacta
     3781 atcctcaccg aactgcatcc cggggtaacg gtcgccgatg tccgcggcaa caccggctgc
     3841 gatttcatcg tcgcgtgaac tggcaggggc cggccgctca gtcggggacc ggcatccgct
     3901 ccggaccctc cttggccgct cgccggcgtc cctggaaccc gcgccaggtc gcgataaacg
     3961 ccggcaagag cgacacgaac aggatgccca ggatgatctt ttccaggttc tggtgcacga
     4021 acgggatgtt gcccaggaag tagccggcca acgtcacgcc gccaccccag aggacgccgc
     4081 cgacgatgtc gaacgccaga tacaccgggt aacgcatgta ggacacgccc gccaccaccg
     4141 gagtgaacgt ccgcacgaac ggcatgaacc gggccaggat gatcgcccac ggtccgtact
     4201 tttcgaagaa ggcgtgtgac tccgtcacgt agtgcttctt gaagaaccgg gaatcttcct
     4261 tcttgaacag cgcagggccg atccgtcgcc cgatgaaata tccggtctgg tcaccgagga
     4321 tcgccaccag cgcgacggag ggcgccagca cccagatatt gagcgtcccg tgtgcggcca
     4381 gcaacccgcc cgtgaacagc agcgactcgc caggcagcag cgggaacagc agaccggtct
     4441 cgacgaaaac gatcaccagg atggtcggca acaccgcagc cgcgaacagg ccctggggac
     4501 cgatccagaa catcgggtcg aggatgctcc ccgaggccat cacggcggtg ctcatgacgt
     4561 cacaacatac cgggactcgt taaccggctc ctaaaaagcg cggccagcgt ggtgttgcga
     4621 tactgagggg accagccgtc aggaggagcc catgcccatc gcaacgcccg aggtctacgc
     4681 cgagatgctg gggcgggcca aggagaactc gtacgccttc ccggccatca actgcacctc
     4741 ttcggagacg gtcaacgccg ccatcaaagg gttcgccgac gccggcagcg acggcatcat
     4801 ccagttctcc accggtgggg cggagttcgc ctcggggctg ggggtcaaag acatggtggc
     4861 cggcgcggtg gcgctggcca agttcacccg cagcatcgcg gccagatacc cgatcaacgt
     4921 cgcactgcac accgaccact gccccaagga caagctggac agctacgtgc ggccgctgct
     4981 ggccatctcg gcggagcggg tggccgccgg caagaacccg ttgttccagt cgcacatgtg
     5041 ggacgggtcg gcggtgccga tcgacgagaa cctcgccatc gccaaggagc tgctcaagac
     5101 ggccgcggcc gccaagatca tcctcgaggt cgagatcggc gtggtgggcg gcgaggaaga
     5161 cggcgtcgag ggcgccatcg acgacaagct gtacaccacc ccggaggact tcgagaagac
     5221 catcgacgca ctgggccacg gtgagcacgg cacgtacctg ctggccgcca ccttcggcaa
     5281 cgtgcacggc gtctacaagc ccggcaacgt caagctgcgc cccgacatcc tggaccaggg
     5341 ccaaaaggtc gcggccgcca agctcgggct gcccgatggc gccaagccgt tcgacttcgt
     5401 cttccacggc ggctccggct cgctgaagtc agagatcgag gaggcgctgc gctacggcgt
     5461 ggtgaagatg aacgtcgaca ccgacaccca gtacgcgttc acccggccgc tggccggcca
     5521 catgttcgcc aactacgacg gcgtgctcaa ggtcgacggc gaagtgggca acaagaaggt
     5581 ctacgacccg cgcagctacc tcaagaaggc cgaggcgggc atgaccgagc gggtgatcga
     5641 ggcctgcaac gacttgcact gcgccggaaa gtcgatcgcc tagccgatgg ggcaccgctg
     5701 cggcacggtg tcgaatgtgc ggctggccgc acattcgaca ccgaacgtgc ggctcgtcgc
     5761 acgctcggca cacgagatcg tcgccgggcg agaacctagc cgctggggga ctggcagatc
     5821 ttccactggt cgtcgcggta ctgcaggtca atgccgcgcg tcgagcggag cgacgggtcg
     5881 tacgccatga acgtggtgat gttcgcctcg gcgtgctggc cgttgacgac gacctggtcg
     5941 atgctggcga tcaccggata ttgcttggcc gccgcgacgc gctggtaggt ctcctgccac
     6001 gcgttttcgt cgtaatccac atacccgtcg cgggcggtcc cgcacgtcat gccgcgcaac
     6061 gcggtcagat cgccccgttg caccgcgacg tcatattcgt ggatggtgtg ccgaacccgg
     6121 tcttcctcag aggttttcgc gtgtttgctg cgggtcagca gcacggtgcc caggatcgcg
     6181 atcgccgcca acgccagcac gatcaggacc agcgccagca cccagcccca gttgaattgc
     6241 ctagatgtcg gcggctttgc tccgggtcga ggcggaatgg attgtggcac ggcggctttc
     6301 gggacagcgc ccagctgcgc cgtggcgcct tccggtcccg gcggcgacgg taatacttcg
     6361 gtggcgggct ccgagctggt ggtgatgacc gtggtttcct tcgcgtcgaa gcccggggca
     6421 gtgaatcgac gctcgcgctg gccttccgga tccgcttcgg gatcgttgtc cggatcggtt
     6481 ctgttgatca ccacggtctc ggtctcggtg tcgcggggca ccagcggttg gtcttcggtt
     6541 tcctcatcgt ggggttcggc ggcccgttgg ccgcttggct cgccccaaga ttcgcgtgga
     6601 tcgaacccag gccgcttcgg cgtaccgcct cggtcgggct cgggtgcatt aggcatgagt
     6661 ggagcttagc gacccgcccc cgggggacgg caaaatagaa accatgacct ctatgggaga
     6721 tctccttgga ccgaaaccga tcctgctgcc cggggatagc gacgccgagg cgcaactacg
     6781 cgccaacgag cacccgagca tcgtcgccgc cgcgcatccg tcggcgtcgg cggcgtgggc
     6841 ggcacttgcc gaagaggcgc tgaccgaaga caaggccgtc acggcctacg cgtacgcccg
     6901 caccggctac caccgcggcc tggaccagct gcggcgaaac ggctggaagg gtttcggccc
     6961 ggtgccctat gcgcatgagc ccaaccgggg cttcctgcgc tgcgtggcgg cattggcgcg
     7021 ggccgccgac gccatcggcg agacccccga gtatcagcgt tgcctggatc tgctcgacga
     7081 ctgcgatccg acggcccgca aagcgctggg cctttagcag gcttcctcgc agtgcccggt
     7141 tccgtccggg cactcgatct ggaccgtggc gtgatccagt ccgcgggccg acagcaccgc
     7201 gcgggcgtcg tggagcaccc gggcggagtc ggcggcactg gtcaggtgtg cggtgcacat
     7261 gtccttgccg ggcgaaagcg tccacacatg caggtcgtgc acttcggtca cgccgtcgac
     7321 ggcacccagc gcggaccgca gctcgtcgac gtcgatgtgg gccggtgaag actcagacag
     7381 gatgcgcagg gcatcgcgcg ccagcgagat ggcccgcggc agcacccaca gcgcaaccag
     7441 cacggccacc acgacgtcgg cgtagggcca atgcgtcgtg acggtgacca cgcccgcgat
     7501 cagcacgccc aggctgccga cggtgtcggc gatcacctcc atgtaggcgc ccttgaccgc
     7561 caggctctgc gacgagtgtg agcgcagcag cagcgccacc acgaagttgg cggccagccc
     7621 ggccagcgcg accacgatca tcggcatgcc ggggaccgag ggggcctcgc tgaggcgctg
     7681 gacggcctcg tagaagatga acaccgcgac cccgatgagc agcgcggcgt tggccaccgc
     7741 ggtgaacacc tccgcgcgat gccagccgta cgtccgcgag ggcgacgagc tgccgcgctg
     7801 ggccaggacc acggcgccca ggcccatgaa cacggcgaca acgtcggtca gcatgtgccc
     7861 ggcatcggcc agcaacgcca tcgagttgat cgacaacgag gtggccaact ccacgacgaa
     7921 gaacgtcgcc aggatcaccg cggcgatgat catgcggggg accatccgag acccgtcggt
     7981 ctcagcgggg gtgtgattgt ggccggcgcc catggccagc aatatatgcg aattaacgca
     8041 tgtattcaag cgtcagaacc agcggctcca gaagcgggtc agcgagttgc ccgcggccgg
     8101 cagccagtgg tgcacaccgg acaggtcgaa cacccagtac accagcccga agatccacgg
     8161 gctggccgcc agcagcagga acagcaggat gaaaaagccg tactgcttgg ccggtgccaa
     8221 cgcgcgctgc gtctcgggac tcaaatgcgg ttccagcgcg tcgtatccgt ccaggccggg
     8281 aatcggcagc aggttcagca acacggcggt gatctgaagg aacgcctgga acgccacccc
     8341 tgcccacagc accgcgtggt tcgggtcgta aaacaaccgg gtcagcacca gcagcagcac
     8401 cgccagcagc aagttggccg ccgggcccgc caggctgacc agggtgcggc gggtgggcgt
     8461 catgaaccag gtccgcaggt acaccgcggc acccggcagg ccgatcccgc ccagcaggat
     8521 caccacgacc ggcatcagca gcgagagcgc gggatggctg tatcgccgcg gatccagcgt
     8581 cagatacccg cggacggcga cgtcatggtc gccgaatcgc caggcggtga aagcgtgccc
     8641 gaattcgtgc aggcacagcg acaccagcca gccggacagc acgaagatga acacgccgac
     8701 ataggacaac ggccgcacgc tcgacccggc cagccaggcc agcacaccgc cgaccaccgt
     8761 caatccgatc agggccaaaa agatggggct gggtcgtacc gattcgcgtt ttcgagcggg
     8821 aaagctcacc ggtcgaaatt accggaccag cagccagcct tcgacgttgc ggtagaacgt
     8881 ggatcgccgc gccgcgcgcg gcgccgggac gccatcacgc accacggtga cgccggtgct
     8941 gcccagctgg gccgcgcggc cggcgatcca ccggcgtccg atcctgctgc cgtacacgct
     9001 ggcccgcagg cccggtgcgg ccgacgtcgg ctcgatgcgc acgccaccga cctccccgtc
     9061 gaagagcggg gtgtcgtcga cgaccgcctc accgtggacg agctgccggt ccgcggccgg
     9121 cagccaatcg gcccggccca cgatcaccga gccggtctcg tcgcggatca gcgggacccg
     9181 acgggcgtga ccccgacgag cgcgtcgcgc cgcccgccgt gccgccggca c
//
LOCUS       DASSGQ010000168         4445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_619808,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000168 DASSGQ010000000
VERSION     DASSGQ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619808"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..358)
                     /locus_tag="VFI55_07500"
     CDS             complement(128..358)
                     /locus_tag="VFI55_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073910.1"
                     /translation="MGELVKEVVVGAIRGALTGAAFGGIGVLLLKGLQNFAILAPYLQ
                     NIEPAMPEEVSTEGAEALTDGVTIIGTSMFNS"
     gene            375..752
                     /locus_tag="VFI55_07505"
     CDS             375..752
                     /locus_tag="VFI55_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073911.1"
                     /translation="MRRYLEPSAMPPATAPHIAPLARARATHNHYLQCQALIGLAGCG
                     RPHRHALDHGWQARRLASAGGYAHLEGQALTTLATVYLDDGQPERAIRMATLAAGIHR
                     RTGHRPGLERSERIVIDARQNLG"
     gene            935..>4445
                     /gene="gltB"
                     /locus_tag="VFI55_07510"
     CDS             935..>4445
                     /gene="gltB"
                     /locus_tag="VFI55_07510"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007297387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HET7073912.1"
                     /translation="MTYSAFPYPQGLYDPANEHDSCGVAFVVDARGRRSHSIVEAGLT
                     ALRNLEHRGAAGSEVNSGDGAGILIQVPDELLRAVTDFPLPGPTGSGEPTYAVGNAFL
                     PADPGQRALAIAVIERILSEERAELVGWRDLPVDAQGADLGPTARSVQPHFAQLFISG
                     DCGLALERLAFVVRRRVERETRQRGVDTYFSSLSARTLVYKGMLTAGQLAPFFADLSD
                     TRLVSAIALVHSRFSTNTFPSWPLAHPYRYIAHNGEINTLNGNRNWMRAREALLESDL
                     IPGDLKRLFPICTPAASDSASFDEVLELLHLGGRSLAHAVLMMVPEAWENNAAMDPAR
                     RAFYEFHASVMEPWDGPACIAFTDGTRVGAVLDRNGLRPARWWRTSGHMVVFASEAGV
                     LDLDPAAVVAKGRLQPGRMFLVDTAAGRIVDDEEIKSELAAEHPYADWLHAGLMRLSE
                     LPDREHITYDHEAVLRRQLTFGYTEEELRVLLTPAARDGVEPIGSMGTDTPIAVLSER
                     PRLLFDYFSQLFAQVTNPPLDAIREEIVTSVALTIGPEQNLLAPSAASCRQIVLPFPV
                     LDNDELAKLIHINDDGDMPGFKAVTVSGLYRVRDGGEGLKVRLIEICRHVSEAIEDGA
                     RLIVLSDRDSTADLAPIPSLLLTACVHHHLIREKTRTMVGLVTEAGDAREVHHMALLL
                     GYGASAVNPYLAFESIDDMIASGAIAGMDPYKAARNYVKAMGKGVLKVMSKMGISTVA
                     SYMGAQVFEAVGLSEVLVERYFTGTSSKLGGIGLSEIHAEVAARHAQAWQANAARGAH
                     RRLETGGEYQWRRDGELHLFNPETVFLLQHSTRTRQFEVFGRYTDAVDSMSRRAATLR
                     GLFDLKVGARPPVPIDEVESVESILTRFSTGAMSYGSISAEAHQTLAIAMNSIGARSN
                     TGEGGEDVDRLYDPTRRSAVKQVASGRFGVTSEYLVNADDLQIKMAQGAKPGEGGQLP
                     AHKVYPWVARTRHSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNANDRARVHVKLVAE
                     VGVGTVAAGVSKAHADVVLISGHDGGTGASPLTSLKHAGVPWELGLAETQQTLLLNGL
                     RDRIVVQVDGQLKTGRDVIIAALLGAEEFGFATAPLVVSGCIMMRVCHLDTCPVGVAT
                     QNPELRKKFDGKPEFVVTFFQYIA"
BASE COUNT          724 a         1532 c         1447 g          742 t
ORIGIN      
        1 atgccgcagc accgcgctgg tgcaccgcga cgctttgtcg tcgcggtgca ccggcgcgct
       61 cgccgtcccg gtcgttcctt aaccgcgatg gtgataaccg cccgagtggg tactggcatg
      121 acctccgtta cgagttgaac atgctggtcc cgataatggt gactccgtcc gtcaacgcct
      181 cggcaccctc ggtgctcact tcctccggca tggccggctc gatgttctgg aggtatggcg
      241 ccaggatggc gaagttctgc aagcccttca atagaagaac gccgatacca ccgaacgccg
      301 caccggtcag cgcacccctg atggcgccga ccaccacctc cttgaccaat tcgcccatgt
      361 cgaccttctc accggtgagg aggtacttgg agccctccgc gatgcctccg gcgaccgcac
      421 cgcatatcgc accgctcgcc cgggcccgcg cgacgcacaa tcactatctg cagtgtcagg
      481 ctctgatcgg gctggccggc tgcggacgcc cgcaccgtca tgccctcgac cacggatggc
      541 aggcgcggcg actggccagc gcgggcggct acgcccatct cgaaggtcag gcgctcacca
      601 cgctggcgac cgtctacctc gatgacggcc agccggaacg cgccatccgg atggcgacgc
      661 tcgccgcggg gatacaccgc cggaccgggc accgccccgg cctcgagcgc agcgagcgca
      721 tcgtcatcga tgcacggcag aacctgggct gatcactcac cacgacgacc ggacctgggg
      781 cgcgaccgtc gcaccaggac cgaccgccac cgcctccggc ttacgaattc gcagatcagt
      841 gtgtatgctc caatgactct gttgggccga tgccgtcccg ccaagctcat gatcaccaca
      901 tgctggcgct tgacgttgat ccggagcccg acacgtgacg tactccgcat tcccgtaccc
      961 tcagggcctt tacgaccccg ccaacgaaca cgactcctgc ggggtcgcgt tcgtcgttga
     1021 cgcacgaggg agacgctcgc actcgatagt cgaggcgggc ctgaccgcgt tgcgcaatct
     1081 tgagcatcgc ggcgcagcgg gttcggaggt caattccggt gacggcgcgg gaatcctgat
     1141 acaggtaccg gacgaattgc ttcgcgccgt gaccgacttc cccttgccgg ggccgaccgg
     1201 gtccggggag cccacctacg ccgtgggcaa cgcgttcctt cccgcggacc ccggccaacg
     1261 ggcccttgcc atcgcggtca tcgagcggat cctctcggag gagcgtgccg aactcgtggg
     1321 gtggcgcgac ctgcccgtcg acgcgcaggg cgccgacctt ggtccgaccg ctcgctccgt
     1381 acaaccgcat ttcgcccagc tcttcatctc gggcgactgc ggccttgccc tggaacggct
     1441 ggcgttcgtg gtgcggcgcc gggtcgagcg ggaaacccgc cagcgtgggg tcgacaccta
     1501 cttctcctcg ctgtccgccc gcacgctggt ctataagggg atgctgaccg cgggtcaact
     1561 cgcaccgttt ttcgctgatc tgtccgacac ccggctggtg agtgcgatcg cgctggtaca
     1621 cagccggttc tccaccaaca cgttcccgag ttggccgctt gcgcatccgt accgctatat
     1681 cgcgcacaac ggcgagatca acaccttgaa cggcaaccgg aactggatgc gtgcacgcga
     1741 ggcactgctc gagagtgacc tgattcccgg cgatctcaag cgactgttcc cgatctgtac
     1801 gcccgcggcg agcgattcag cgagtttcga cgaggtcctc gaactgctgc atctgggcgg
     1861 ccgctcgctg gcgcacgccg tgctgatgat ggttccggag gcgtgggaga acaacgccgc
     1921 gatggacccg gcgcgacgcg cgttctacga gttccacgcc tcggtgatgg agccgtggga
     1981 cggtccggcg tgcatcgcgt tcaccgacgg gacccgggtc ggcgcggtcc tcgaccgtaa
     2041 cggtctgcgc ccggcccggt ggtggcgtac cagcggccac atggtcgtgt tcgccagcga
     2101 ggccggtgtc cttgacctcg acccggccgc ggtcgtcgcg aagggccggc tgcagcccgg
     2161 gcggatgttc ctcgtggata cggccgccgg gcgcatcgtc gacgacgagg agatcaagtc
     2221 tgagctggcc gccgagcacc cgtacgccga ctggctgcac gccggtctga tgcggctgtc
     2281 cgagttgccg gaccgggaac acatcaccta cgaccacgag gcggtgctgc gccgccagct
     2341 cacgttcggc tacaccgagg aggagttgcg ggtcctgctc accccggcgg cccgcgatgg
     2401 tgtcgagccg atcggatcga tgggcacgga caccccgatc gcggtgctgt ccgagcgccc
     2461 gcgactgctc ttcgattact tcagccagtt gttcgcgcag gtgacgaacc cgcccctgga
     2521 cgcgatccgg gaggagatcg tcacctcggt cgccttgacg atcgggccgg agcagaacct
     2581 gctcgcaccg tcggcggcgt cgtgccgtca gattgtgctc ccgtttcccg tgttggacaa
     2641 cgacgagctg gccaagctca tccacatcaa cgacgacggc gacatgcccg ggttcaaggc
     2701 ggtcaccgtg tcgggcctgt accgggtacg cgacggcggt gagggtctca aggtccgtct
     2761 gatcgagatc tgccgacacg tctccgaggc gatcgaggac ggcgcacggt tgatcgtgct
     2821 gtccgatcgc gactcgaccg cggacctggc accgatcccg tcgctgctgc tcaccgcctg
     2881 cgtccaccac catctcatcc gcgaaaagac ccgcacgatg gtcggtctgg tcacggaggc
     2941 gggcgacgct cgcgaggtgc accacatggc cctgttgctc ggctacggcg cttccgccgt
     3001 gaacccgtat ctcgcgttcg agtccattga cgacatgatc gccagcggcg cgatcgccgg
     3061 catggatccg tacaaggccg cgaggaacta cgtcaaggcc atgggcaagg gtgtcttgaa
     3121 ggtgatgtcg aagatgggca tctccaccgt cgcctcctac atgggcgcgc aggtcttcga
     3181 agcggtcggc ctgtccgagg tcctcgtcga gcggtacttc accgggacat ccagcaagct
     3241 cggtgggatc gggctatcgg agatccatgc cgaggtcgcc gcccgtcacg cgcaagcctg
     3301 gcaggcgaat gccgcccgcg gcgcccaccg ccggctggaa acgggcgggg agtatcagtg
     3361 gcgccgcgac ggcgaactgc acctgttcaa ccctgagacg gtgttcctgc tgcagcactc
     3421 gacccgcacc cggcagttcg aggtgttcgg caggtacacc gatgccgtgg actcgatgtc
     3481 ccggcgggcg gcgaccctgc gcggcctgtt cgacctgaag gtcggcgccc gcccaccggt
     3541 gccgatcgat gaggtcgagt cggtcgagag catcctcacg cggttctcca ctggcgcgat
     3601 gtcctacggt tccatctccg ccgaggcgca ccagacgctc gcgatcgcga tgaactcgat
     3661 cggcgccaga tccaacaccg gcgagggtgg cgaggacgtc gatcggctct acgaccccac
     3721 ccggcgcagc gccgtcaaac aggtcgccag tggacggttc ggtgtcacca gcgagtacct
     3781 ggtcaacgcc gacgacctcc agatcaagat ggcgcagggt gccaaacccg gcgagggcgg
     3841 gcagttgccc gcgcacaagg tctatccgtg ggtggcccgc acgcggcact ccacgccggg
     3901 cgtggggctg atctctccac cgccacacca tgacatctac tccatcgagg atctggcaca
     3961 gctgatccac gacctgaaga acgccaacga ccgggcccgt gtccacgtga aactcgtggc
     4021 cgaggtcggc gtaggcacgg tcgcggccgg ggtgagcaag gcgcacgccg atgtggtcct
     4081 catctccggc cacgacggtg gcaccggcgc gtcgccgttg acctcgctca agcatgccgg
     4141 tgtgccgtgg gagctgggcc tggccgagac gcagcagacg ctgcttctca acggactgcg
     4201 cgaccggatt gtcgtgcagg tcgacggcca gctcaagacc ggccgtgacg tgatcatcgc
     4261 cgcgctgctg ggcgcggaag agttcggctt cgcgaccgca ccactggtgg tgtcgggctg
     4321 catcatgatg cgggtgtgtc acctggacac gtgcccggtc ggcgtggcca cgcaaaatcc
     4381 ggagctgcgc aagaagttcg acggcaagcc cgagttcgtg gtcacgttct tccagtacat
     4441 cgccg
//
LOCUS       DASSGQ010000169        13432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1044853,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000169 DASSGQ010000000
VERSION     DASSGQ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13432
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044853"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /gene="cydC"
                     /locus_tag="VFI55_07515"
     CDS             complement(<1..1520)
                     /gene="cydC"
                     /locus_tag="VFI55_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HET7073913.1"
                     /translation="MAAIALGVLSLGSALALAGFSAWLITRASQMPPVLDLSVAVVAV
                     RTFAISRGVLHYCERLATHDTALRAAGTARVQIYRRLAHGPAAAAVRLHSGELVARVG
                     TDVDELANVLVRAVVPIGVAAVLAVAATAVVAVISLPAAAVLAICLLIAGYVAPRLAA
                     RAAAAQEGIARQHHSDRDTSAMIALEHAPELRVAGALPGVIAESQRRQHAWGAALDAA
                     ARPAAIAEAMPTAAIGASVLGAVVAGIGLAHTVAPTTLAVLMLLPLSAFEAMTPLPAA
                     AVQLTRSRIAARRLLDLAPKDPGDGGKSAVTAPGGTGRLSADISSGHADAAQPSRAEL
                     DLLPGTRLAVTGASGAGKTTMLMTLAGLMPPLDGRVLLDGTDLSEFDEDELHTTVSFF
                     AEDAHIFATTVRDNLLVARGDCQDDELASVLDSVGLAAWRAGLPEGLSTVLIGGAQAL
                     SAGQRRRLLLARAIVSPARIVLLDEPTEHLDAIDAERLLSDLLGPDPRLMSAERTVV"
     gene            complement(1666..3279)
                     /gene="cydD"
                     /locus_tag="VFI55_07520"
     CDS             complement(1666..3279)
                     /gene="cydD"
                     /locus_tag="VFI55_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HET7073914.1"
                     /translation="MRRYLVATVGCGVVISGCAIGSAIVLASIVAHVVGDASARNLRG
                     WLVPLSILLALWVVRTVTHWLQARLGQRGASAVIADLNGQVLAAVTARQPADLAAQRD
                     AAAVVVTRGLDGLRPYFTGYLPTLLLAAILTPATVAVIACYDLKSTVIVAITLPLIPI
                     FMVLIGLATADRSAAALAAMTTLQGRLLDLIAGIPTLRALGRGRGPERRIAELAAAHR
                     RSAMATLRIAFLSALVLELLATLGVALIAVGIGLRLVFGEMTLTAGLTVLLLAPDVYW
                     PLRRIGVEFHAAQDGRTAADSAFALIGEQAAQQVRDQTVVARGAQLRLDKLGVTGRDG
                     CAPCDLTAVIEPGWVTVLHGPNGAGKSTTLQAIAGVTVPSSGRVTVAGIDVADLEQAA
                     WWRQLSWLPQRPALVPGTVYDNLVLLGDLPDLEAACAASGFDAVLAELPDGMDTVLGR
                     GGVGLSLGQRQRLGLARALGSPAAVLLLDEPTAHLDAHTEDRVLRAIVERARGGATVV
                     VVGHRAPVVAIGDRVVEVSAESGVRYAPV"
     gene            complement(3342..4382)
                     /gene="cydB"
                     /locus_tag="VFI55_07525"
     CDS             complement(3342..4382)
                     /gene="cydB"
                     /locus_tag="VFI55_07525"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HET7073915.1"
                     /translation="MGLQELWFGIIAALFLGFFILEGFDFGVGMLMDPLARISSGDRE
                     INRRAILNTIGPVWDGNEVWLITAGAGMFAAFPGWYATVFSTLYLPLLAILFGMILRV
                     VSIEWRGKIDDPKWRAWANFGIATGSWLPAVLWGVAFATLVRGLPVEANHRVRPSVGD
                     ILNAYTLLGGLATASLFLFYGAVFVTLKTAGPIRDDAYRFAMWISLPVTGIVAGFGLW
                     TQLAHGKDWTWAVLGVAVVAQLVAVLLVWRRASDAWAFACTAVVVAAVVILLFGALYP
                     NLVPSTLDSQWNLTIYNSSSTPYTLKIMTWVTAFFAPLTVVYQAWTYWVFRQRISADK
                     IPPPIGLARHPS"
     gene            complement(4413..5885)
                     /locus_tag="VFI55_07530"
     CDS             complement(4413..5885)
                     /locus_tag="VFI55_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET7073916.1"
                     /translation="MNVVDISRWQFGITTVYHFIFVPLTIGLAPLVAIMQTVWVVTGN
                     ATWYRLTKFFGKLFLINFAIGVATGIVQEFQFGMNWSEYSRFVGDVFGAPLAMEGLAA
                     FFFESTFLGLWIFGWSKLPRLVHLACIWIVAIAVNASAFFIITANSFMQHPVGAHYNP
                     VTRRAELDSIGALLSNNTAQAAFSHAVVGAWLTAGTFVAAVSAWWLVRSNKSGSDAPA
                     DESTRTIFRPAALLGCWVVLVAAFGLGLTGDFQGKLMFKQQPMKMASAESLCDTETDP
                     DFSVLTVGRQNNCDSLYRVMQVPYVLPFLAQSKFDGVTLQGVRNIQQQDEQRFGPNDY
                     RPNLFVTYWSFRAMIGFMAIPGLFALATLWLTRGGRIPQRKRWFPWLALLTIPTPFLA
                     NSAGWVFTEMGRQPWLVVPNPTGDQQLRLMVAQGVSDHPAAVVITSLVTFTLLYAVLG
                     LIWFWLLKRYVAEGPLEHDAEPAAPPAPGDDEVAPLSFAY"
     gene            complement(5997..6152)
                     /locus_tag="VFI55_07535"
     CDS             complement(5997..6152)
                     /locus_tag="VFI55_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073917.1"
                     /translation="MQTSPTTPVPSLLPHLWKSTLVSGVVWFVIIGVFEIVSSFGIRN
                     ASKPLSR"
     gene            complement(6200..7531)
                     /locus_tag="VFI55_07540"
     CDS             complement(6200..7531)
                     /locus_tag="VFI55_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7073918.1"
                     /translation="MAAKKCGASSNDADGSARRPDCVAAVRAQTRDRTQHYADIAARR
                     ARLLTITAWLSVMVLASFVFVQIFTGAWVWQITATNVGASIIFASVPWLHRFGELVAP
                     LTFIGAAYVSITATCWEVGTGSGAQFFFVVGACVVVLLLGIEHIVLASALAAVAAGLV
                     IALQFLAPHDTGLQPAWATSTGFVITAVSSVVMVVVTVWFALRDTARAEAVMEAQYER
                     SETLLANMLPASIAERLKEPDRGVIADKYDDASVLFADIVGFTERASSSAPADLVRFL
                     DRLYSAFDALVDKHGLEKIKVSGDSYMVVSGVPRPRPDHAQALADFALGMANAAAGLK
                     DSHGRAVPLRVGMACGPVVAGVVGSRRFFYDVWGDAVNVASRMESTDSVGRIQVPEAM
                     YEHLKDEFVLQERGHIDVKGKGPMRTWYLIGRKATEEPTDLLTEEPRTVHV"
     gene            complement(7599..7672)
                     /locus_tag="VFI55_07545"
     tRNA            complement(7599..7672)
                     /locus_tag="VFI55_07545"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7636..7638),aa:Leu,seq:caa)
     gene            7795..8427
                     /locus_tag="VFI55_07550"
     CDS             7795..8427
                     /locus_tag="VFI55_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="HET7073919.1"
                     /translation="MTGPTTDADAAVPRRVLIAEDEALIRLDLAEMLREEGYEIVGEA
                     GDGQEAVELAARHKPDLVIMDVKMPRRDGIDAASEIATKRIAPIVVLTAFSQRDLVER
                     ARDAGAMAYLVKPFTISDLIPAIELAMSRFSEITALEREVATLSDRLETRKLVERAKG
                     LLQAKQGMTEPEAFKWIQRAAMDRRTTMKRVAEVVLETLGDAPKEESRDS"
     gene            complement(8533..9732)
                     /locus_tag="VFI55_07555"
     CDS             complement(8533..9732)
                     /locus_tag="VFI55_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HET7073920.1"
                     /translation="MSPEPLYILGAGMHPWGKWGRDFTEYGVVAARAALAEAGLDWRQ
                     IQLVAGADTIRNGYPGFIAGSTFAQKLGWNGVAVSSSYAACASGSQALQDARAQILAG
                     FCDVALVIGADTTPKGAFAPVQGERKNDPDWQRFHLIGAMNPVYFALLARRRMDLYGA
                     TAEDFAQVKVKNSRHGLQNPHARYRKESSVDDVLASPMVADPLRQLDICATSDGAAAL
                     IVASAKFTRQHLGSLDGVPSVRAVSTVTPRYPQHLPELPDIATDSTAVVPAPERVFKD
                     QILDAAYAEAGIGPEDLSLAEVYDLSTALELDWYEHLGLCGKGGAEALLRSGATTIGG
                     KVPVNPSGGLACFGEAIPAQAIAQVCELTWQLRGQATGRQVENATVGVTANQGLFGHG
                     SSVIVAR"
     gene            complement(9741..10232)
                     /locus_tag="VFI55_07560"
     CDS             complement(9741..10232)
                     /locus_tag="VFI55_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HET7073921.1"
                     /translation="MPEVTSRQPAIDGWFATDDAGNPHLVGSKCPRCGTYVFPPRKNN
                     CPNPACAGDTLEPVALSTRGTLWSYTENRYPPPAPDPAPDPFEPFAIAAVELAHEGLI
                     VLGKVVEGTLAADLKVGMEMELTTMPLFTDDAGVQRTVHAWRRADDDAVGAPPACGGE
                     RRK"
     gene            10379..13063
                     /gene="polA"
                     /locus_tag="VFI55_07565"
     CDS             10379..13063
                     /gene="polA"
                     /locus_tag="VFI55_07565"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET7073922.1"
                     /translation="MLLDGNSLAFRAFYALPAENFKTRGGLTTNAVYGFTAMLINLLR
                     DESPTHIAAAFDVSRQTFRSERYPEYKANRSSAPDEFRGQIDITKEVLGALGITVLSE
                     PGFEADDLIATLATQAEDEGYRVLVVTGDRDSLQLVSDDVTVLYPRKGVSELTRFTPE
                     AVVEKYGLTPTQYPDFAALRGDPSDNLPGIPGVGEKTASKWIVEYGSLQGLVDNVDSV
                     RGKVGDALRANLASVVRNRELTDLVKDVPLAQTPDTLRLQPWDRDQIHRLFDDLEFRV
                     LRDRLFETLAAVEPEVDEGFDVRGGALEAGTVGRWLAEHAGDGSRSGLAVVGTHLPHD
                     GDATALAVAAADGDGAYIDTATLTPDDDAALAAWLADPAKPKALHEAKLAIHDLAGRG
                     WTLNGVTSDTALAAYLVRPGQRSFTLDDLSLRYLRRELRAETPEQQQLSLLDDLDELG
                     GVDEQAVQTLILRSRAVVDLADALDAELDRIDSTALLGDMELPVQRVLADMESAGIAV
                     DLRLLTGLQSQFGDQIRDAAEAAYAVIGKQINLGSPKQLQVVLFDELGMPKTKRTKTG
                     YTTDADALQTLFDKTGHPFLQHLLAHRDVTRLKVTVDGLLQAVATDGRIHTTFNQTIA
                     ATGRLSSTEPNLQNIPIRTDAGRQIRDAFVVGKGPDGPFAELMTADYSQIEMRIMAHL
                     SRDEGLIEAFNTGEDLHSFVAARAFGVRIEEVTAELRRRVKAMSYGLAYGLSAYGLAA
                     QLKISTEEAKVQMDQYFARFGGVRDYLMDIVEQARKDGYTSTVLGRRRYLPELDSSNR
                     QVREAAERAALNAPIQGSAADIIKVAMIEVDRAIKEAGLASRMLLQVHDELLFEIAPG
                     EREQVETLAREKMGGAYPLDVPLEVSVGYGRSWDGAAH"
     gene            13263..>13432
                     /gene="rpsA"
                     /locus_tag="VFI55_07570"
     CDS             13263..>13432
                     /gene="rpsA"
                     /locus_tag="VFI55_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216146.1"
                     /note="in Escherichia coli this protein is involved in
                     binding to the leader sequence of mRNAs and is itself
                     bound to the 30S subunit; autoregulates expression via a
                     C-terminal domain; in most gram negative organisms this
                     protein is composed of 6 repeats of the S1 domain while in
                     gram positive there are 4 repeats; the S1 nucleic
                     acid-binding domain is found associated with other
                     proteins; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET7073923.1"
                     /translation="MPSPAVTSPQVAVNDIGSSEDFLAAIDKTIKYFNDGDIVEGTIV
                     KVDRDEVLLDIG"
BASE COUNT         2444 a         4415 c         4526 g         2047 t
ORIGIN      
        1 acgacggtgc gttccgcgga catcagccgt ggatccgggc ccaacaggtc gctgaggagc
       61 cgttcggcgt cgatcgcatc gaggtgctcg gtgggctcgt ccagcagcac gattcgggcg
      121 ggcgaaacga tcgcgcgagc cagcaacagc ctcctgcgct ggccggccga gagtgcttgc
      181 gcgccaccga tcagcaccgt tgacagacct tcgggcaaac cggcgcgcca cgcggcgagg
      241 ccgacggaat ccagcaccga cgccagctcg tcgtcttgac agtctccgcg ggccaccagg
      301 agattgtctc ggacggtggt ggcgaagatg tgggcgtctt cggcgaaaaa gctgacggtg
      361 gtgtgtagtt cgtcttcgtc gaattcgctc aggtcggtac cgtcgagcag cacccgccca
      421 tcgagcgggg gcatcagtcc ggcgagagtc atcagcatcg tcgtcttgcc ggcgccgctg
      481 gcgccggtga cggccagcct ggtgcccggc agcaggtcaa gctccgcgcg gctcggttgg
      541 gcggcgtcgg cgtggccgga agaaatatcc gcggacagcc tgccggtgcc gccgggtgcc
      601 gtcaccgccg acttcccgcc gtcacccgga tcttttggcg ccaggtcgag caggcgccgc
      661 gcggcgatcc gcgaccgcgt caactggaca gcggccgccg gcaacggcgt catcgcctcg
      721 aatgcggaca gcggcaacaa catcaggacc gccagggtgg tgggcgcaac cgtgtgtgcc
      781 aacccgatcc cggccaccac ggcgcccagc acactggctc cgatcgcagc ggtcggcatt
      841 gcctcggcga tggcggccgg tctggcggcc gcgtccagcg cagcgcccca agcatgttgg
      901 cggcgttgcg actcggcgat gacaccgggc agtgcgcctg cgacgcggag ctccggcgcg
      961 tgttcgagcg cgatcatcgc cgacgtatcc cggtcggaat ggtgttggcg cgcaatccct
     1021 tcctgtgctg ccgccgccct ggcggcaagt cgcggtgcga cgtagcccgc gatcagcaga
     1081 cagatcgcca acaccgcggc ggccggcagc gaaatgaccg cgactactgc cgtagccgcc
     1141 accgccaata ccgccgcaac gccgatcggc accacggcac gcaccagaac attggccagt
     1201 tcgtcgacgt cagtgcccac ccttgccacc agctcaccgc tgtgcagccg gacggcggcc
     1261 gcggccggtc cgtgtgccag ccggcggtag atctgtaccc gggcggtgcc ggcggcgcgc
     1321 aacgcggtgt cgtgggtggc cagtcgctcg cagtagtgca atacgccccg tgatatcgcg
     1381 aacgtgcgca cggcgacgac cgcgacggac aggtccagca cgggcggcat ctgggaggcc
     1441 cgcgttatca gccaggccga aaaaccggcc agggccagcg cgctgcccag tgacagcacc
     1501 cccaacgcga tggccgccat gatgcggggc agccgcgggc gcagcagggg caaggcggcc
     1561 aacaggtcgc tctggcggcg acccgccgtg ctggcgatcg ccgcgtttgc gctctggcgg
     1621 cgacccgcgg cgcccggctt cgccgcgctg gcgatcgccg cgggttcaga ccggggcata
     1681 acgcaccccg ctctcggccg acacctcgac gacccggtca ccgatcgcga caacgggcgc
     1741 gcggtgcccg acgacgacca cggtcgcgcc cccgcgtgcg cgctcgacga tggcccgcaa
     1801 gacccggtct tcggtgtggg cgtcgaggtg cgcggtcggt tcgtcgagca gcagcaccgc
     1861 ggccggtgaa ccgagcgcac gggccaggcc cagccgctgg cgctggccga gtgacagccc
     1921 gacgccgccg cgtccgagca ccgtgtccat cccgtcgggc agctcggcca gcacggcgtc
     1981 gaatcccgat gcggcgcagg ccgcttcgag gtccggcaag tccccgagga ggaccaggtt
     2041 gtcgtacacg gtgccgggga ccagcgcggg gcgctgcggc agccacgaca gctgccgcca
     2101 ccacgcagcc tgctctaaat cggcgacgtc gatcccggcg acggtgacgc ggcccgatga
     2161 gggcacggtg actcccgcga tcgcctgcag cgtggtgctc ttgccggcgc cattggggcc
     2221 gtgcagcacg gtcacccagc cgggctcgat caccgcggtg agatcgcagg gcgcgcagcc
     2281 gtcccggccg gtcacgccga gcttgtccag acgtagctgc gcgccgcgcg ccaccactgt
     2341 ctggtcgcgg acctgctgcg ccgcctgctc gccgatgagt gcgaaggcgc tgtcggcggc
     2401 cgttcggccg tcttgagcgg catggaactc cacgccgatc cgacgcagtg gccagtacac
     2461 atccggagcc agcagcagga ccgtcagacc ggcggtcagg gtcatctcac cgaacaccag
     2521 gcgaaggccg atgccgaccg cgatcaatgc cacccccagc gtggccaaca attcgagcac
     2581 cagcgccgac aggaaggcga tgcgcagcgt cgccattgcc gaacgtcgat gggcggcagc
     2641 cagttcggcg atccggcgct ccgggccgcg gccgcggccc agcgcacgca gcgtggggat
     2701 accggcgatc aggtccagca gccgcccctg cagtgtcgtc atcgcggcca gggcggccgc
     2761 cgagcggtcg gccgttgcca gcccgatgag caccatgaaa atcgggatca acggcagggt
     2821 gatcgccacg atcaccgtcg acttcaggtc gtagcaggcg atcacggcaa cggtcgccgg
     2881 ggtcaggatc gcggcgagca gcaaggtggg cagatagccg gtgaagtaag gacgcaaccc
     2941 gtccaggccg cgggtgacca cgaccgcggc ggcatcacgc tgtgcggcga ggtcggcggg
     3001 ctgccgggcg gtcaccgcgg ccagcacctg accgttgagg tctgcaatca ccgcgctggc
     3061 cccccgttga cccaggcgtg cctgtagcca gtgcgtcacc gtccggacga cccacagcgc
     3121 caacaggatt gacaggggta ccaaccagcc gcgcaggtta cgtgccgagg catcgccgac
     3181 gacgtgcgcg acgatgctcg ccagcacgat cgcggatccg atcgcgcagc cggagatcac
     3241 cactccgcag cccaccgtgg cgaccagata gcgtcgcagg gcggtcgacg tccgccacag
     3301 tcgcgggtcc agggggccgc gtcttgctga gtccttcgtg ttcaggacgg gtgccttgcc
     3361 aggccaatcg gcggcggtat cttgtcagcc gagatccgtt gccgaaagac ccaatacgtc
     3421 cacgcctggt acaccaccgt caacggggcg aagaacgccg tcacccaggt catgatcttc
     3481 agggtgtagg gcgtcgagga ggagttgtag atcgtcaggt tccattggct gtcgagggtt
     3541 gacggcacca ggttggggta cagcgcaccg aacaacagga tcaccacggc cgcaacgacc
     3601 accgcggtac acgcgaacgc ccaggcatcg gatgcacgcc gccacaccaa cagcaccgcc
     3661 accagctggg cgaccaccgc gacaccgagc acagcccaag tccagtcctt gccgtgcgcc
     3721 agttgtgtcc aaagtccaaa gcccgcaaca attcccgtga cgggaagcga tatccacatc
     3781 gcgaatcgat aggcgtcgtc gcggatcggg cccgcggtct tcagcgtcac gaacaccgcg
     3841 ccgtagaaca agaacaggct ggcggtggcc aggccgccca gcagggtata ggcgttgagt
     3901 atgtcgccga ctgacgggcg cacacgatgg ttggcttcta cggggagtcc gcggaccaga
     3961 gtggcgaagg ccacgcccca cagcaccgcg ggcaaccacg acccggtcgc gatgccgaaa
     4021 ttggcccagg cccgccactt cgggtcatcg attttgccgc gccattcgat cgacacgaca
     4081 cgcaggatca tgccgaacag aatcgcaagc agcggcaggt acagggttga gaagacggtg
     4141 gcgtaccagc cgggaaacgc ggcgaacatc cctgcgccgg cggtgatgag ccagacttca
     4201 ttaccatccc agaccgggcc gatggtgttg aggatcgcgc gccggttgat ctcccggtca
     4261 cccgagctga tgcgcgcgag cggatccatc aacatgccta cgccgaagtc gaagccttcc
     4321 aggatgaaga aacccagaaa cagcgccgcg atgatgccaa accacaattc ttggagtccc
     4381 accggtcagc ccctttctcc gatcagtagg cgtcaatatg cgaatgagag cggggcgacc
     4441 tcgtcatcgc cgggtgccgg cggcgcagca ggttcggcgt cgtgttccag cggtccctcg
     4501 gcgacgtagc gcttgagcag ccagaaccag atgagcccaa gcaccgcgta gagcaaggtg
     4561 aacgtcacca gcgaggtgat gacgacggca gcagggtgat cggagacgcc ctgggcgacc
     4621 atcagccgaa gctgctggtc gccggtcggg ttgggcacca cgagccaggg ctgacggccc
     4681 atctcggtga atacccaccc ggcgctgttg gcgaggaacg gggtgggaat ggtcagcagt
     4741 gccagccagg gaaaccaccg cttcctctgg ggaattcggc caccgcgggt aagccacagc
     4801 gttgccagcg cgaagagccc gggaatcgcc atgaacccga tcatcgcgcg aaaagaccaa
     4861 taggtgacga acaggttggg ccgatagtcg ttgggcccga atcgctgttc gtcctgctgc
     4921 tggatgttgc gcacgccctg caacgtcacg ccgtcgaatt tgctctgggc gaggaagggc
     4981 aacacgtagg gcacttgcat cactcggtag aggctgtcgc agttgttctg cctgccgacc
     5041 gtcaggaccg agaaatccgg gtcagtttcg gtgtcgcaca acgattccgc cgacgccatc
     5101 ttcatcggtt gttgcttgaa catcagcttg ccctggaagt caccggtcag gcccaagccg
     5161 aatgcggcca ccaacacgac ccaacatccc aggagggcag ccgggcggaa gatggttcgg
     5221 gttgactcgt cggccggtgc gtcggatccg gatttgttgg aacggaccag ccaccaggca
     5281 ctgacggcgg ccacgaaggt ccctgccgtc agccacgcac cgaccaccgc atgtgaaaac
     5341 gcggcttgcg cagtgttatt gctcagcaat gccccgatgc tgtccaactc ggcgcgccgc
     5401 gtcacggggt tgtagtgggc gccgaccgga tgctgcatga aggagttcgc ggtgatgatg
     5461 aagaacgccg aggcattgac cgcgatcgcg acgatccaga tgcacgccag atgcaccagc
     5521 cggggcagct tgctccagcc gaagatccac aacccgagga aggtggactc gaagaagaag
     5581 gcggcgagcc cttccatggc cagcggggcg ccgaacacgt cgccgacgaa gcgcgagtat
     5641 tcgctccaat tcatgccgaa ctgaaactcc tgcacgatcc cggtcgccac cccgatcgcg
     5701 aagttgatca ggaacagctt gccgaagaac ttggtgaggc gataccacgt ggcgtttccg
     5761 gtaaccaccc acactgtctg catgatcgcg accaggggag ccaaaccgat ggtgagcggc
     5821 acgaaaatga agtggtagac ggtagtgata ccgaactgcc accgcgaaat gtcgacgaca
     5881 ttcatttgtc atttccccaa actgcggagg ctcattgacc agcaatgact taatacagct
     5941 acgacgaagt gtagtagacg agcttgggca agtgctaccg gcaatgttgt gccgctctaa
     6001 cggctcagcg gcttggacgc gttgcggatg ccgaacgacg acacgatttc gaacactccg
     6061 atgatcacga accacacaac ccccgatacc agcgtggatt tccacaagtg cggcaacaag
     6121 cttggaacag gggtcgtagg actggtttgc atggccgcag tctgacacaa acgcaggtcc
     6181 tggtataggg caactctgct cagacgtgta ccgtgcgcgg ttcctcggta agcaggtcgg
     6241 tgggttcttc ggttgccttg cggccgatca gataccaggt gcgcatgggg cccttgccct
     6301 tgacgtcgat gtggccccgc tcctgcagca cgaattcgtc tttgaggtgt tcgtacatcg
     6361 cctcgggcac ctggatgcgt cccaccgagt cggtggattc catccgggat gcgacgttga
     6421 ccgcgtcccc ccacacgtca tagaagaacc ggcgagaacc cacgacaccc gcgaccaccg
     6481 gtccgcaggc catgcccacc cgaagcggga cggcacggcc gtgcgaatcc ttcagtccgg
     6541 ctgccgcatt cgccataccc agcgcgaaat cggcgagggc ttgcgcgtgg tcggggcgcg
     6601 ggcgtggtac tccgctgacc accatgtagg aatcgccgct caccttgatt ttctccagcc
     6661 cgtgcttgtc caccagcgca tcgaaagcgc tgtacaagcg gtcgaggaag cgcaccaggt
     6721 cggccggcgc gctgctgctc gcacgttcgg tgaatccgac gatgtcggcg aacaagaccg
     6781 aggcgtcatc gtatttgtcg gcgatgacgc cccgatcggg ctccttgagt cgctcggcga
     6841 tgctggccgg caacatgttg gccagcaacg tctcggaacg ctcgtactgg gcttccatga
     6901 ccgcctcggc gcgcgcggtg tcgcgcagcg cgaaccacac cgtcacgacc accatcacga
     6961 cgctggagac ggccgtgatc acgaaacccg tcgacgtggc ccacgccggc tgcagcccgg
     7021 tgtcgtgggg ggccaggaac tgcagggcga tgaccaggcc ggcggcgacc gccgcgagcg
     7081 ccgacgccaa cacgatgtgt tcgatcccca gcagcagcac caccacgcag gcgcccacca
     7141 cgaagaagaa ctgggcgccg gaaccggtgc ctacctccca gcaggtggcg gtgatcgaaa
     7201 cataggccgc gccgataaac gtcagcggcg caaccaattc gccgaaacgg tgcagccacg
     7261 gaacgctcgc gaagatgatc gaggcgccga cgttggtcgc ggtgatctgc cagacccacg
     7321 ctccggtgaa aatttgcacg aaaacaaaac tggcgagcac catcacggac agccaggcag
     7381 tgatggtgag caaccgagcc cggcgtgcgg cgatatcggc gtagtgctgt gtgcggtcgc
     7441 gggtttgtgc gcgcactgcc gcgacacagt ccggccggcg cgccgaaccg tctgcatcgt
     7501 ttgatgaggc accgcacttc ttcgcggcca cgatcaaagc ctaaccgttg agctggcggt
     7561 ttaccgaccg tgccggtgaa aatcgcgcgc atgcggggtg ccctcggtga gattcgaact
     7621 cacactggac gggttttgaa tccgtttcct ctgccagttg ggatacgagg gcttgaaagg
     7681 cggggcggtt ccctacctta aggcagcctc ccaccataga ggatcggtga gatcgacggt
     7741 tctcaccgcc ccccgacgtg gctggtcgca gccgtccacg acacaatgtc cgtcatgaca
     7801 ggccccacga ccgacgccga cgccgctgtg ccgcgccggg tcctgatcgc ggaagacgaa
     7861 gcgctgatcc gactggactt ggccgagatg ctgcgagagg agggttacga aatcgtcggt
     7921 gaggcgggtg acggccagga agccgtcgag ttggccgcgc gccataagcc cgacctggtg
     7981 atcatggatg tgaagatgcc gcgccgtgac ggcatcgacg cggcatcgga aatcgccacc
     8041 aagcgcatcg cgccgatcgt ggtgctgacc gcgttcagcc agcgcgactt ggtcgaacgc
     8101 gctcgcgacg ccggggccat ggcctatctg gtgaagccct tcaccatcag cgacctgatc
     8161 ccggccattg aattggccat gagccggttc agcgagatca ccgcactgga acgtgaggtg
     8221 gcgacgctgt ctgaccggct ggagacccgc aagctggtcg aacgcgccaa gggtctgctg
     8281 caggccaaac agggcatgac cgagcccgag gcgttcaagt ggatccagcg cgccgccatg
     8341 gaccgccgca ccaccatgaa acgggtggcc gaggtcgtcc tggaaaccct cggggatgcg
     8401 ccgaaagagg agtccaggga cagctgagcc gccgaggcgt cgagtgtact gacggcgttt
     8461 ggtgtgcgct cagggcgacg acacgccgcg cggggccgct ttggtttcac actcagcgga
     8521 gagaaaaccc atctaccggg cgacgatcac cgaggacccg tggccgaata ggccctgatt
     8581 ggccgtgacg ccgacggtgg cgttctccac ctgccggccg gtggcctgac cgcgcagctg
     8641 ccaggtcagc tcgcagacct gagcgatggc ctgggcgggg atggcctcac cgaagcaggc
     8701 cagcccgccc gacgggttga ccggcacctt gccgccgatg gtggtggcac cgctgcgcag
     8761 cagcgcctcg gccccaccct tgccgcacag ccccaggtgt tcgtaccagt ccagctccag
     8821 cgcggtggac aggtcgtata cctcggccag gctgaggtcc tcgggcccga tgcccgcttc
     8881 ggcgtaggcc gcgtcgagga tctgatcctt gaatacccgt tccggtgcgg gcacgacggc
     8941 ggtggaatcg gtggcgatgt cgggcaattc gggcaggtgc tgggggtagc gcggggtcac
     9001 cgtgctcacc gcacgcaccg aaggaacgcc gtcgagcgag ccgaggtgtt ggcgagtgaa
     9061 tttcgcgctg gcgacgatca gcgcggccgc cccgtcggag gtggcgcaga tatcgagctg
     9121 tcgcaaggga tcggccacca tcggactggc cagcacgtcg tcgaccgagg attccttgcg
     9181 gtagcgggca tgcggattct gcaggccgtg gcgagagttc ttgactttca cctgggcgaa
     9241 gtcttccgct gtcgcgccgt acaggtccat ccggcgccgc gcgagcagcg cgaagtacac
     9301 cgggttcatc gccccgatca ggtggaaccg ctgccagtcg gggtcgttct tgcgttcgcc
     9361 ttgcaccggg gcgaacgctc ccttgggggt ggtgtcggcg ccgatgacca gcgcgacgtc
     9421 gcagaacccg gccaggatct gtgcccgggc atcctgcagg gcctgcgaac cgctggcgca
     9481 cgcggcgtag ctggagctga ccgcgacgcc gttccacccg agtttctgcg cgaacgtcga
     9541 cccggcgatg aagccggggt acccgttgcg aatggtgtcc gcgccggcga ccagctggat
     9601 ctggcgccag tccaggccgg cctcggcgag tgcggcgcgc gcggcgacca ctccatattc
     9661 ggtgaagtcc cggccccact tcccccacgg gtgcatgccg gcgccgagga tgtagagggg
     9721 ttcgggactc atgaggctcg tcatttgcgc cgctctcccc cgcaagcggg tggtgccccc
     9781 actgcatcgt cgtcggcgcg cctccacgcg tgcaccgtgc gctgcacacc ggcgtcgtcg
     9841 gtgaacagcg gcatcgtggt cagctccatc tccatgccga ccttcaggtc ggccgccagc
     9901 gtgccctcga ccacctttcc cagcacgatc agcccctcgt gggccagctc gacggcggcg
     9961 atggcgaagg gctcgaaggg gtccggcgcg gggtcgggcg ccggtggggg ataacgattt
    10021 tccgtgtagc tccacagcgt tccccgagtg gacaatgcga cgggctctag tgtgtcgccg
    10081 gcgcaggcgg ggttggggca gttgttctta cgcggcggga acacgtaggt gccgcatcgg
    10141 gggcacttgc tgccgaccag gtgtgggttg cctgcgtcgt cggtggcgaa ccatccgtcg
    10201 attgccggtt gtcggctggt gacctcgggc actcggccag actaccgagc cgagagccaa
    10261 aactgaaacg tgttgcagtt ctgtggacgg cacggtcggg gcgcgtggga cgtcacaccg
    10321 ggtgcctaga gtgtgagccg tgagcaccgc cagcggagac caacccaagc cgacgctgat
    10381 gctgctggac ggcaactcgc tggcgtttcg ggctttctat gcgctgcccg cggagaactt
    10441 caagacccgc ggcgggctga ccacgaacgc ggtgtacgga ttcaccgcga tgctgatcaa
    10501 cctgctgcgc gacgagtctc cgacgcatat cgcggcggcg ttcgacgtgt cgcggcagac
    10561 cttccgctcc gagcgttacc cggaatataa ggccaaccgg tcgtcggccc ccgacgagtt
    10621 ccgcggtcag atcgacatca cgaaggaagt gctgggcgcg ctgggcatca ccgtgttgtc
    10681 agagccggga ttcgaagccg acgacctgat cgccacgctg gccacccagg ccgaagacga
    10741 gggctaccgg gtgctggtgg tcaccggtga ccgcgactcg ctgcagctgg tcagtgacga
    10801 cgtcactgtg ctgtacccac gcaaaggcgt cagcgaactg acccgtttca cccccgaggc
    10861 cgtcgtcgaa aagtacgggc tgacgcccac ccagtacccc gacttcgccg cgctgcgcgg
    10921 cgatcccagc gacaacctgc ccgggattcc cggtgtgggg gagaagaccg cctccaagtg
    10981 gatcgtggag tacggctcgt tgcaggggct ggtggacaac gtcgactcgg tgcgcgggaa
    11041 ggtgggcgac gcgctgcggg ccaatctggc cagcgtggtg cgcaaccggg agctcaccga
    11101 cctcgttaaa gacgtgccgc tggcgcaaac cccggacacg ctgcggctgc agccgtggga
    11161 tcgcgaccag atccaccggc tcttcgacga cctggagttc cgggtgttgc gcgaccggtt
    11221 gttcgaaacc ctggccgcgg tcgaacccga ggtcgatgag ggttttgacg tgcgcggtgg
    11281 agcgctggag gccggcacgg tcgggcggtg gctggccgag cacgccggcg acgggagccg
    11341 gtccgggctg gcggtggtgg gtacccatct gccgcacgac ggggacgcca ccgcgctggc
    11401 cgtcgccgcc gccgacggcg atggcgccta catcgatacc gcgacgttga cacccgacga
    11461 cgacgccgcg ctggcggcgt ggctggccga tcccgcgaaa ccgaaagcgc tgcacgaggc
    11521 caagctggcc atccacgacc tggccggccg tggctggacg ctgaacgggg tcacctcaga
    11581 caccgcgctg gcggcctacc tggtgcggcc gggtcagcgc agcttcaccc tcgacgatct
    11641 ctcgctgcgt tacctgcgtc gcgagctgcg cgcggagaca cctgagcagc aacaactttc
    11701 gttgctagac gacttggacg agttgggcgg ggtggacgag caggccgtcc agacgctgat
    11761 tctgcggtcc cgggcggtgg tggatctggc cgacgcgctg gacgcagagc tggaccggat
    11821 cgattccacg gcattgctgg gggacatgga gctgccggtt cagcgggtgc tggcggacat
    11881 ggaaagcgcc ggcatcgccg tcgatttgcg gctgctgacc gggttgcaaa gccaattcgg
    11941 cgaccagatc cgcgacgccg ccgaggccgc ctacgccgtg atcggcaagc agatcaactt
    12001 gggctcaccc aagcagttgc aggtggtgtt gttcgacgaa ctcggaatgc cgaagaccaa
    12061 gcggaccaaa accggctaca ccaccgacgc cgacgccctg caaacgctgt tcgacaagac
    12121 cgggcatcct ttcctgcaac acctgctggc gcaccgcgac gtcacgcgat tgaaggtcac
    12181 cgtcgacggg ctgctgcagg cggtcgccac ggacggccgg attcacacca cgttcaacca
    12241 gacgatcgca gcgaccggcc gactttcctc aacggagccc aacctgcaga acatcccgat
    12301 ccgcaccgac gcggggcgcc agatccggga cgcgttcgtg gtggggaagg gccccgacgg
    12361 gccgtttgca gagctgatga ccgccgacta cagccagatc gagatgcgga tcatggcaca
    12421 cctgtcgaga gatgaaggct tgatcgaagc ctttaacaca ggggaggacc tgcactcgtt
    12481 cgtcgccgca cgggctttcg gcgtccggat tgaagaggtg accgccgagt tgcgtcgccg
    12541 ggtcaaggcg atgtcgtacg ggctggccta cgggttgagc gcctacgggc tggccgccca
    12601 gctgaaaatc tccaccgagg aggcaaaagt ccagatggac cagtacttcg cccggttcgg
    12661 cggggtgcgc gactacttga tggacatcgt cgagcaggcc cgcaaggacg gctacacctc
    12721 gaccgtgctg ggccgccggc gttaccttcc cgagctggac agcagcaacc gccaggtgcg
    12781 ggaggccgcc gagcgggcgg cgctcaacgc gcccatccag ggcagcgcgg ccgacatcat
    12841 caaggtggcg atgatcgagg tcgacagggc gatcaaggag gctgggctgg cgtcgcggat
    12901 gttgctgcag gtgcacgacg aattgctgtt cgaaatcgca cccggtgaac gcgaacaggt
    12961 cgagacgctg gcccgggaga agatgggcgg tgcctacccg ctcgacgtcc cgctcgaggt
    13021 ctcggtgggt tacggccgca gctgggatgg cgccgcgcac tagagggacc cgctgggacg
    13081 cgatcaggcc ggccggggta gcgtcaaggc gacaaatcca gcgggagctc gccgcggcgt
    13141 gctttcgcgc ccgggtgcgg cgcaccggcc gagtttgtcc agcgtgcgcc ctgtcgagta
    13201 gcctcgacag gtaaatccca ttttgtccct acgacccaac ctgtccggag caacccaaca
    13261 acatgccgag tcccgccgtc acctcgccgc aagtagccgt caacgacata ggctccagcg
    13321 aggactttct cgcagcaata gacaaaacga tcaagtactt caacgatggc gacatcgtcg
    13381 aaggcaccat cgtcaaagtt gaccgggacg aggtgctcct cgacatcggc ta
//
LOCUS       DASSGQ010000170         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1075449,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000170 DASSGQ010000000
VERSION     DASSGQ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075449"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VFI55_07575"
     CDS             complement(<1..719)
                     /locus_tag="VFI55_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HET7073924.1"
                     /translation="MTDNQLASQTPRSPLELLDTVPESVLRRLKQYSGRLATQAVSAM
                     QDRLPFFAGLEASQRASVALVVQTAVVNFVEWMHDPHSNVSHTAKAFELVPQELTRRI
                     ALRDSVDMVRVTMEFFEEVVPLLARSDEQLTALTVGILKYSRDLAFSFASVYADAAEA
                     RGSWDSRMEASVVDAVVRGDTGPELLSRAAALNWDTTAPATVVVGTPAPDRDGSDGAG
                     GSQRASQLVRDVAVRHGRAALT"
     gene            complement(805..>2223)
                     /locus_tag="VFI55_07580"
     CDS             complement(805..>2223)
                     /locus_tag="VFI55_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HET7073925.1"
                     /translation="SDAELDADPYLPPYYHPGPDAPELRYMLDRRRTLGGFVPQRRTK
                     AKTLKLPGREVYRALKKGSGSQEVATTMATVRTFKELLRDKEIGPRIVPIIPDEARTF
                     GMDSWFPSLKIYNRLGQLYTAVDADLMLAYKESEIGQILHEGINEAGSVGSFTAVGTS
                     YATHDEPMIPIYIFYSMFGFQRTGDGFWAAADQMARGFVLGATAGRTTLTGEGLQHAD
                     GHSLLLAATNPAVISYDPAFAFEIAHIVESGLARMFGENPQNVYFYITIYNEPYVQPP
                     EPDNFDPDGVLRGLYRYHTATGPHAHKAQILASGVAMPAALKAAQLLAAEWDVATDVW
                     SVTSWGELNRDGVRIERAKLRHPDQPADVPYVTQALADARGPVIAVSDWMRAVPEQIR
                     PWVPGTYLTLGTDGFGFSDTRPAARRYFNTDAESQVVAVLEALARDGEIDPSIPITAA
                     SHYRIDDVQAAPKQTSDPGAGA"
BASE COUNT          326 a          685 c          823 g          389 t
ORIGIN      
        1 gtgagcgccg cacgcccgtg acggacggcg acgtcgcgga cgagttggct ggcccgctgg
       61 ctgccgccgg caccgtccga gccgtcgcgg tcgggtgccg gggtgccgac cacgaccgtc
      121 gccggcgcgg tggtgtccca gttcagtgcg gccgcacgag atagcagctc ggggccggtg
      181 tcgccgcgga ccaccgcgtc gacgacgctg gcctccatcc ggctgtccca gctgccccgt
      241 gcttcggcgg cgtcggcgta gacggaggcg aagctgaacg cgaggtcgcg gctgtacttc
      301 aggatgccca ccgtcagggc ggtcagctgc tcgtcggagc gggccagtag cggcacgact
      361 tcctcgaaga actccatggt cacgcgcacc atgtccacgc tgtcgcgcaa tgcgatccgg
      421 cgggtgagtt cctggggtac cagctcgaag gctttcgcgg tgtggctgac gttgctgtgc
      481 gggtcgtgca tccattcgac gaagttgacg acggccgtct gcaccaccag cgcgacgctg
      541 gcccgctggg aggcttccag gccggcgaag aacggcaacc ggtcctgcat ggccgagacc
      601 gcctgggtgg ccagccggcc ggaatactgc ttcagccgac gcagcacgga ctcgggcacg
      661 gtgtccagca gttcgagcgg cgacctcggg gtctgcgacg ccaactggtt gtcagtcacc
      721 cctaaaatct acgcctgatt tctggaacta ttctccaacg caacgctggg actttaagaa
      781 cgacttatgg gcgacgcgag gcggttatgc ccccgcgccg gggtcggagg tctgtttggg
      841 ggcggcttgt acgtcgtcga tgcggtagtg gctggcggcg gtgatgggta ttgaggggtc
      901 gatttcgccg tcgcgggcca gggcttcgag gaccgcgacg acctgggatt cggcgtcggt
      961 gttgaagtag cggcgtgcgg cgggtcgggt gtcggagaag ccgaacccgt cggtgcccag
     1021 ggtgaggtag gtgccgggca cccagggtcg gatctgttcg gggaccgcgc gcatccagtc
     1081 cgatacggcg atcaccggcc cgcgggcatc cgcgagggcc tgggtgacgt aggggacgtc
     1141 ggcgggctgg tcggggtggc gcagcttggc gcgctcgatg cgtacgccgt cgcggttgag
     1201 ttcgccccag ctggtcaccg accacacgtc ggtggcgacg tcccactcgg cggccagcag
     1261 ctgtgcggct ttcagcgcgg cgggcatggc cacccccgag gccaggatct gtgccttgtg
     1321 ggcgtgtggc ccggtggcgg tgtggtagcg gtagaggccc cgcagcacgc cgtcgggatc
     1381 gaagttgtcc ggctcggggg gctgcacgta gggctcgttg taaatggtga tgtagaagta
     1441 cacgttctgc gggttttccc cgaacatgcg ggccagcccg ctttccacga tgtgggcgat
     1501 ctcgaaggcg aacgcggggt cgtaggagat caccgccgga ttggtggcgg ccagcagcaa
     1561 cgagtggccg tcggcgtgtt ggaggccctc accggtcagc gtggtgcgac ccgcggtcgc
     1621 ccccagcacg aagccgcgcg ccatctggtc ggcggcggcc cagaaaccgt cgccggtgcg
     1681 ttggaagccg aacatggaat agaagatgta gatcgggatc atcggctcgt catgggtggc
     1741 ataggaggtg cccaccgcgg tgaacgagcc caccgacccc gcctcgttga tgccctcgtg
     1801 caggatctgc ccgatttcgc tttccttgta ggccagcatc agatcggcgt ccaccgcggt
     1861 gtagagctgg ccgaggcggt tgtagatctt caggctcggg aaccacgagt ccatcccgaa
     1921 ggtgcgggcc tcatcaggaa tgatcggcac gatgcgtggc ccgatctcct tgtcgcgcaa
     1981 cagttccttg aaggtgcgca ccgtggccat ggtggtagcc acctcttggc tacccgaacc
     2041 ctttttcagc gcccggtaga cctcgcggcc cggcagtttg agcgttttgg ccttggtgcg
     2101 gcgctgcggg acgaagcccc ccagcgtgcg gcgccggtcg agcatgtagc gcagctccgg
     2161 ggcgtccggg ccggggtggt agtagggcgg caggtaggga tcggcgtcca gctcagcgtc
     2221 gct
//
LOCUS       DASSGQ010000171         4714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1258201,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000171 DASSGQ010000000
VERSION     DASSGQ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258201"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1440)
                     /locus_tag="VFI55_07585"
     CDS             complement(73..1440)
                     /locus_tag="VFI55_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7073926.1"
                     /translation="MNTQALAELIAALPDGMVVTDPTITEGYRQDRAFDPSAGKPLAV
                     VRPRRTSEVQTVLRWATAHRVPVVPRGAGSGLSGGATALDNGIVLSTEKMRDISVDPI
                     TRTAVCQPGLFNAEVKKAVAEHGLWYPPDPSSFEICSIGGNIATNAGGLCCVKYGVTT
                     DYVLGVQVVLADGTAVRLGGPRLKDVAGLSLTKLFVGSEGTLGVITEVTLRLLPAQNV
                     SSVVVATFASVQAAVDAVLGVAARLRPSMLEFMDSVAINAVEDTLRMDLDREAAAMLV
                     AGSDERGRGGSQDAEVIAAVFAENGAKEVFSTDDPDEGEAFVAARRFCIPAVEAKGSL
                     LLEDVGVPLPALGDLVHGIELIAAERELMISVIAHAGDGNTHPLIVYDPGDAAMTERA
                     HLAFGEIMDLAVGLGGTITGEHGVGRLKRPWLDGYLGPEAMDLNRRIKQALDPLGILN
                     PGAAI"
     gene            complement(1461..2708)
                     /locus_tag="VFI55_07590"
     CDS             complement(1461..2708)
                     /locus_tag="VFI55_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7073927.1"
                     /translation="MKSSSRGPALLILFATLMATAGTGISIVAFPWLALQHRDSARDA
                     AIVATAMTLPLVVSTLVAGTAVDFFGRRRISLVSDSLSGAAVAAVPLIAAAFGPDTIN
                     LLVLAVLAALAAAFDPAGTTARQSMLPEAAARAGWSLDRTNSIYEAILNLAFIVGPGV
                     GGVMIATVGGVNTMWVTAGLFGLSFLAIGALRLEGTGKPHRATRPEGLLSGIAEGMRF
                     VWSLRVLRTLGLIDLVVTALYLPMESVLFPKYFADQHQPAQLGWALMALGAGGVTGAL
                     GYAVLSKYTRRRTAVLTATLTFGAATVGIAFLPPLPVILVLCAVTGVVYGPIQPIYNY
                     VMQTRAPHRLRGRVVGVMTGLTYAAGPLGLLVAGPLADAAGLKATFLTLAVPILLVGL
                     IACGLPSLRELDPSPEFADDLGS"
     gene            2791..3618
                     /locus_tag="VFI55_07595"
     CDS             2791..3618
                     /locus_tag="VFI55_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET7073928.1"
                     /translation="MQDVHVLVHPNTGQAFGSPVAPGSGWPGDPATPQTPVAADAAEV
                     TTLAARAASIPDLNALVSVCGACPRLVTWREDVAVSKRRAFADQPYWGRPVPGWGSER
                     PWLLIVGLAPAAHGANRTGRMFTGDRSGDQLYAALYRAGLVNSPISVDAADGLRANQI
                     RIVAPVRCAPPANAPTPTERDTCWPWLDAEWRLVSDHVRVIVALGGFAWQIALRLAGV
                     SGVRKPRFGHEAVAELEAGVRLLGCYHPSQQNMFTGRLTPVMLDDVFSNAKRLAGIK"
     gene            3625..>4714
                     /locus_tag="VFI55_07600"
     CDS             3625..>4714
                     /locus_tag="VFI55_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET7073929.1"
                     /translation="MSSVLVSGASVAGMTAAYWLAQQRHSVTVVERYPGLRPGGQAID
                     VRGPALTVLERMWLRAAADHLRTRMRGASVVDRDGNELPSDTESTPIGGINANPDIEV
                     LRDDLVELLYEAAKLRVEYLFDDSITALADDGTSVAVTFEREVERSFDLVIGADGLHS
                     KVRELVFGEEAQFIEGLGTYAAIFTVPNFLDLDYWQTWHYGDSTMAGVYSVRDNTEAR
                     AMLGFMDTDLQIDYRDTEAQFDELERRMAEDGWVRPQLLRYMRTAPDFYFDEMSQVKM
                     DRWSKGRVALVGDAAYCCSPLSGQGTSVALLGAYILAGELAAAGDDYELGFTNYHKEF
                     QDYVKRNQWLVVDNIPGGAPIPQEVFDRI"
BASE COUNT          799 a         1567 c         1591 g          757 t
ORIGIN      
        1 ttgacgccac gccgatgcgg aattacgacg tgctcaaccg cttcgcgttc tgagccgcgg
       61 ggcgagcgac gctcagatgg ccgcgccggg gttgaggatg ccgagcgggt ccaatgcctg
      121 cttgatgcgc cggttgaggt ccatcgcctc gggcccgaga tagccgtcca gccagggccg
      181 cttcaaccgg ccgacgccgt gctcaccggt gatcgtgccg cccaggccga cggccaggtc
      241 catgatttcg ccgaacgcga gatgggcgcg ctcggtcatg gcggcgtcgc cggggtcgta
      301 cacgatcaag gggtgggtgt tgccatctcc ggcgtgcgcg atcaccgaga tcatcagttc
      361 gcgttcggcg gcgatcagct caatcccatg gaccaagtcg cccagcgccg gcagcggcac
      421 cccgacgtct tccagcagca gcgatccctt ggcttccact gccggaatgc agaaccggcg
      481 ggcggccaca aacgcctcgc cctcgtccgg atcgtcggtc gaaaagactt ctttcgcacc
      541 gttttccgcg aacaccgcag caatcacctc ggcgtcctgg ctgccgcccc gcccacgctc
      601 gtcggagccg gccaccagca tcgcggccgc ctcgcggtcc aggtccatgc gcagggtgtc
      661 ctcgacggcg ttgatcgcga ccgaatccat gaactccagc atcgaaggtc gcagccgcgc
      721 agcgactccc agcaccgcgt cgacggccgc ctggaccgag gcgaaggtcg ccaccacgac
      781 gctggacacg ttttgcgcgg gcagcaaccg cagcgtcact tccgtgatga cgcccagcgt
      841 gccctcgctg ccgacgaaca gcttggtcag gctcagccct gcgacgtcct tgagccgggg
      901 tccgcccagc cggaccgcgg tgccgtcggc cagcactacc tgcacgccca gcacgtaatc
      961 ggtcgtgacg ccgtatttca cgcagcacaa gccgccggcg ttggtggcga tgttgccgcc
     1021 gatgctgcag atctcgaacg acgacggatc cggcggatac cacagtccgt gctcggcgac
     1081 ggcctttttc acctccgcgt tgaacaggcc gggctggcat accgcggtgc gggtgatcgg
     1141 gtcgacgctg atgtcgcgca tcttctccgt cgacaacacg atgccgttgt ccagtgccgt
     1201 cgccccgccg gacaggccgc tgccggcccc ccgcgggacc accggcaccc ggtgggcagt
     1261 ggcccagcgc agcacggtct ggacttcgct ggtgcgccgc ggccggacca cggccagggg
     1321 tttgcccgcc gacgggtcga aagcgcggtc ctggcggtag ccctcggtga tggtgggatc
     1381 ggtgaccacc atcccgtcgg gcagcgcggc aatcagctca gccagcgcct gtgtgttcat
     1441 agggcgtgca cggcccgatc ttacgacccg agatcgtcag caaactccgg cgaggggtcc
     1501 agctcgcgca gcgacggtag cccgcacgcg atgaggccga ccaacaggat cgggaccgcc
     1561 aacgtcagga acgtcgcctt cagtccggcg gcgtcggcca ggggacccgc gaccagcaag
     1621 cccaacggcc ccgcggcgta ggtcaacccc gtcatcaccc cgaccacgcg gccacgcagc
     1681 cgatgcggag cccgggtctg catgacgtag ttgtaaatcg gctggatcgg cccgtagacc
     1741 acgccggtca ccgcgcacag caccaggatc accggcaagg gcggcaggaa cgcgatgccg
     1801 acggtcgccg caccgaaggt gagggtcgcc gtcagcaccg cggtacgccg ccgcgtgtat
     1861 ttcgacagca cggcatagcc gagcgcgccc gtcacgccgc cggccccaag cgccatcagc
     1921 gcccaaccca actgtgccgg ttgatgttgg tcggcgaagt atttgggaaa cagcacgctt
     1981 tccatcggca ggtacagcgc ggtgacgacc aggtcgatca atccgagcgt ccgcagcacc
     2041 cgcagactcc acacaaatcg cattccttcg gcgatgccgg acagcagccc ttcgggtcgc
     2101 gtcgcgcggt gtggcttgcc ggtgccctcc agccgcagcg ccccgatcgc gaggaaggat
     2161 aagccgaata ggccggcggt gacccacatc gtgttgacac cgccaaccgt cgcgatcatc
     2221 acaccgccga cgcccgggcc cacgatgaac gccagattga gaatcgcttc gtagatgctg
     2281 ttggtgcggt ccagcgacca gcccgcgcgg gccgcggcct cgggcagcat cgactggcgc
     2341 gccgtggtcc cggccgggtc gaaagcggcc gccagcgccg ccaggacggc aagcaccagc
     2401 aggttgatcg tgtctggacc gaacgcggcg gcgatcagtg gcacggcggc caccgctgca
     2461 cccgacagcg aatcggagac cagtgaaatc cggcggcggc cgaaaaagtc gactgcggtg
     2521 ccggcgacca gcgtggagac gaccagcggc agcgtcatgg cggtggccac gatggccgcg
     2581 tcgcgcgcgc tgtctcggtg ctgcaacgcc agccaaggga aggcgacgat cgagatgccg
     2641 gtacccgccg tcgccatcag ggtggcgaaa aggatcaaga gtgcggggcc ccggctgctg
     2701 cttttcatgg gatatcgccg ctgaatttat gccaaaccag tatctgcggc gacccaattt
     2761 cggggcattt ccgcgccggc tatcgcgtga atgcaggatg ttcatgtgct cgtccatccg
     2821 aataccggcc aggcgttcgg ttcgcccgtt gcgcccggca gcggatggcc gggtgatccg
     2881 gccacaccgc agacgccggt ggccgccgac gccgcagagg tcaccacgct ggctgcgcgg
     2941 gccgcgtcga taccggacct caatgctctg gtcagcgtct gcggcgcctg cccgcgtctg
     3001 gtgacgtggc gcgaggacgt cgcggtcagc aagcgccgag cgtttgctga ccagccgtat
     3061 tgggggcggc cggtgccggg ctggggatcg gagcggccct ggctattgat cgtcggactg
     3121 gcgccggccg cgcacggcgc caatcggacc gggcggatgt tcaccggtga ccgctcgggc
     3181 gaccagttgt acgcggcgct gtaccgggct ggtctggtga attcaccgat cagcgtggac
     3241 gccgcggacg gattgcgggc caaccagatt cggatcgtcg cgccggtgcg gtgcgccccg
     3301 ccggccaacg ccccgacgcc gacggagcgc gacacgtgct ggccctggct ggatgccgaa
     3361 tggcggctgg tgtccgatca tgttcgcgtc atcgtggccc tgggcggctt cgcctggcag
     3421 atcgcgttgc gattggcggg tgtgtcggga gttcgcaagc cgcgattcgg gcacgaagcc
     3481 gttgcggagc tggaagccgg cgtgcgattg ctcggctgct accaccccag ccagcaaaat
     3541 atgttcaccg gtaggttgac tccggtaatg ctcgacgacg tcttcagcaa cgcgaagagg
     3601 ctggcaggga tcaagtgact tttcatgtct agtgtgctgg tatccggtgc cagcgtggcc
     3661 ggtatgaccg ctgcgtattg gctcgcccag cagcgccatt ccgtcaccgt cgtggagcgc
     3721 tatcccgggc ttcggccggg cggtcaggcg atcgatgtgc ggggtccggc gctgacggtg
     3781 ctggaacgca tgtggttgcg ggcggccgcc gaccatctga ggacgcgaat gcggggtgcg
     3841 tcggtcgtcg accgcgacgg caacgaactt cccagcgaca ccgaatcgac acccatcggc
     3901 gggatcaacg ctaaccccga cattgaggtg ctacgcgacg atctcgtcga attgctttac
     3961 gaggcagcca aactaagggt cgagtacctg ttcgatgaca gcattaccgc gttggcggac
     4021 gacggtacct cggtcgctgt gacctttgaa cgcgaagtcg aacgcagctt cgacttggtg
     4081 atcggcgcgg acggattgca ctccaaggtc cgcgaattgg tcttcggcga ggaggcgcag
     4141 ttcatcgagg ggctgggcac ctatgccgcg attttcacgg tgcccaactt tctggacttg
     4201 gactattggc agacgtggca ctacggggac tcgaccatgg cgggcgtcta cagcgtgcgc
     4261 gacaacaccg aggcccgggc catgctgggg ttcatggata ccgacctgca aatcgactac
     4321 cgcgacaccg aggcgcagtt cgacgagttg gaacggcgga tggctgaaga cggctgggtt
     4381 cgcccacaac tgctgcgata tatgcggacc gcaccggact tctacttcga cgagatgtcg
     4441 caggtcaaga tggatcgctg gtcaaagggc agggtggccc tggtcggcga cgccgcgtac
     4501 tgctgttcgc cgttgtcggg ccaggggacc agcgtcgcgc tgttgggggc ctacatcctg
     4561 gccggtgagc tcgccgcagc cggcgacgac tacgagctgg gcttcaccaa ctaccacaaa
     4621 gagttccagg actacgtcaa gcgcaaccaa tggctggtgg tcgacaacat ccctggtggt
     4681 gcgccgattc cgcaggaggt gttcgaccgc atcg
//
LOCUS       DASSGQ010000172        25781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_772097,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000172 DASSGQ010000000
VERSION     DASSGQ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 25781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25781
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772097"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VFI55_07605"
     CDS             complement(<1..989)
                     /locus_tag="VFI55_07605"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HET7073930.1"
                     /translation="MNVSDRFPTPRPSPAGRVRVAVVFGGRSNEHAISCVSAGSILRN
                     LDPQRFDVIAIGITPEGSWVLTDGDPDALAITNRQLPGVTAESGTELALPADPRRGGQ
                     LVSLSPAAGPAAGEVLASVDVVFPVLHGPYGEDGTIQGLLELAGVPYVGAGVLASAAG
                     MDKEFTKKLLVAEGLPVGPHVVLRPVRAALRPEECERLGLPVFVKPARGGSSIGVSRV
                     SSWDQLPGAVAHARRHDPKVIIEAAVNGRELECGVLEMPDGTLEASTVGEIRVAGVRG
                     REDAFYDFATKYLDDAAELDVPAKVDDDIADAVRQLAIRAFKAIDCQGLARVD"
     gene            complement(1069..2088)
                     /locus_tag="VFI55_07610"
     CDS             complement(1069..2088)
                     /locus_tag="VFI55_07610"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET7073931.1"
                     /translation="MGSRSGAVAVMGAGAWGTALAKVLAEAGGPEAEVTLWARRSSVA
                     EQINATRYNPAYLPGTLLPPGIRATADADEALAGASTVLLGVPAQTMRSNLERWAPSL
                     AAGATLVSLAKGIELGTLMRMSQVIVSVTGVDPTQVAVISGPNLASEIAQCQPSATVV
                     ASSDSGRAVALQRMLNSGYFRPYTNSDVVGTEVGGACKNVIALACGMAAGVGLGENTA
                     AAIITRGLAEIIRLGIALGAKGATLAGLAGVGDLVATCTSPQSRNRSFGECLGRGGTM
                     ESAMEARDSHVVEGVTSCEAVLALASSYDVEMPLTDAVHRVCHRGLSVDEAMALLLGR
                     STKPE"
     gene            2218..2889
                     /gene="cofC"
                     /locus_tag="VFI55_07615"
     CDS             2218..2889
                     /gene="cofC"
                     /locus_tag="VFI55_07615"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HET7073932.1"
                     /translation="MSGRRADGAGDGAGDRGDVALIIAVKRLAAAKTRLAPVFSAPTR
                     ESVVLAMLVDTLTAAARVPALGSITVITPDEAAAAAAAELGAGVLADPTATGHGDPLN
                     NAIAAAEQAVAGAFANVVALQGDLPALQSQELAEAIAAARNHRRSFVADRLATGTAAL
                     CAFGTPLDPRFGPDSAARHRRSGAIELTGAWPGLRCDVDTPADLAAARRLGVGAATAR
                     AITQH"
     gene            2980..5187
                     /locus_tag="VFI55_07620"
     CDS             2980..5187
                     /locus_tag="VFI55_07620"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HET7073933.1"
                     /translation="MMSNDRMVTEIEAEARPDENLWHSGESAVAAPPAATPATSADEL
                     PEDRYLNRELSWLDFNARVLALAADSSLPLLERAKFLAIFASNLDEFYMVRVAGLKRR
                     DEMGLSVRSADGLTPREQLRRIGEQTQQIASRHARVFLDSVRPALAEEGIHIVTWADL
                     DKAEREQLSTYFTEQVFPVLTPLAVDPAHPFPFVSGLSLNLAVTVRQPEDGGQHFARV
                     KVPDNVNRFVELAVREAGKENSAGTEGKTVIRYLPLEELIAAFLPVLFPGMEIVEHHA
                     FRITRNADFEVEEDRDEDLLQALERELARRRFGSPVRLEIADDMTESMLELLLRELDV
                     HPGDVIEVPGLLDLSSLWQIYGLDRPALKDAAFVPATNPAFADRETPKSIFATLREGD
                     VLVHHPYDSFSTSVQRFIELAAADPNVLAIKQTLYRTSGDSPIVRALIAAAEAGKQVV
                     AMVEIKARFDEQANIRWARTLEQAGVHVVYGYVGLKTHCKTCLVVRREGSTIRRYCHV
                     GTGNYNGKTARLYEDLGLLTASPEIGADLTDLFNSLTGYSRKVSYRNLLVAPHSIRAG
                     IIERVEREVAAHRESGDGHIQLKMNSLVDEQVIDALYRASQAGVRVEIVVRGICALRP
                     GAEGFSENVLVRSILGRFLEHSRIIRFGRINEFWIGSADMMHRNLDRRVEVLVQVKDP
                     RLTTYLNELFESALDPSTRCWELGSDGQWTASPQAGHTVRDHQVWLMERHTSP"
     gene            5288..6235
                     /locus_tag="VFI55_07625"
     CDS             5288..6235
                     /locus_tag="VFI55_07625"
                     /EC_number="3.1.3.-"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NUDIX hydrolase/histidine
                     phosphatase family protein"
                     /protein_id="HET7073934.1"
                     /translation="MSTQNSSAGRRSGSRIVYAAGAVLWRPAGAGPANADLEIAVIHR
                     PRYDDWSLPKGKVDPGETAPVAAVREVFEETGHHAILGRRLDLVSYPIEQGIKKVYYW
                     AARSIGGEFTPGREVDELVWLPVADAMPKLNYAQDRKMLQHFTKKPADTHTVLIVRHG
                     TAGQKSRFSGDDTKRPLDKRGRAQAEALVPQLLAFGASDVYAADRLRCQQTVEPLAEE
                     LGVAVHDEPTLTEESYAKSPKRGRNRMLKIAEQDGTPAICTQGKVIPDLIAWWCERDG
                     VRPDKSRNHKGSTWVLSLSAGRLVAADHIGGALAANVRA"
     gene            complement(6305..6943)
                     /locus_tag="VFI55_07630"
     CDS             complement(6305..6943)
                     /locus_tag="VFI55_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET7073935.1"
                     /translation="MNKAELIDVLTQKLGSDRRQATAAVENFVDTIVRAVHKGDSVTI
                     TGFGVFEQRRRAARVARNPRTGETVKVKPTSVPAFRPGAQFKAVVSGAQRLPAEGPAV
                     KRGVVAGVAAKKAAKKAPAKKTATRAPAKKAATKAPAKKAVTKAPVKKAVTKAPVKKA
                     AVKAPVKKAAAKAPVKKAVTKSASKAPAKKVAAKRPASKAPAKKATPRRGRK"
     gene            complement(7156..7752)
                     /gene="leuD"
                     /locus_tag="VFI55_07635"
     CDS             complement(7156..7752)
                     /gene="leuD"
                     /locus_tag="VFI55_07635"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET7073936.1"
                     /translation="MEPFRTHTGIGVPLRRSNVDTDQIIPAVFLKRVTRTGFEDGLFA
                     TWRSDPSFVLNLSPFDRGSVLVAGPDFGTGSSREHAVWALMDYGFRVVISSRFGDIFR
                     GNSGKAGLLAAEVSQDGVELLWKLIEQNPGLEITANLQDRNITAGTTVLPFKIDDYTA
                     WRLLEGLDDIALTLRKLDKIEEFEATYPEWKPRTLPAS"
     gene            complement(7775..9241)
                     /gene="leuC"
                     /locus_tag="VFI55_07640"
     CDS             complement(7775..9241)
                     /gene="leuC"
                     /locus_tag="VFI55_07640"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HET7073937.1"
                     /translation="MGSDISPAAGKDGPAKPRTLAEKVWEDHVVVSGSSGDEASAPDL
                     IYIDLHLVHEVTSPQAFDGLRLAGRPVRRPDLTLATEDHNVPTTGVDNLIADLVSRTQ
                     VETLRRNCAEFGIRLFPMGNIEQGIVHVVGPQLGLTQPGMTIVCGDSHTSTHGAFGAL
                     AMGIGTSEVEHVMATQTLPLRPFKTMAVNVDGTLPAGVTAKDIILTLIAKIGTGGGQG
                     HVIEYRGSAIESLSMEGRMTICNMSIEAGARAGMVAPDATTYEFLRGRPHAPTGAQWD
                     AALEYWQQLGTDPGAVFDTEVYLDAASLSPFVTWGTNPGQGVPLDAAVPDPELMTDDG
                     ERQAAEKALAYMDLRPGTPMRDISVDTVFVGSCTNGRIEDLRVVADVLRGRKVAEGVR
                     MLVVPGSMRVRAQAEDEGLGEVFTAAGAEWRQAGCSMCLGMNPDQLSPGQRCASTSNR
                     NFEGRQGKGGRTHLVSPVVAAATAVRGRLSTPADLNSN"
     gene            9330..10031
                     /locus_tag="VFI55_07645"
     CDS             9330..10031
                     /locus_tag="VFI55_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET7073938.1"
                     /translation="MRQHSGIGVLDKAVGVLHTIAESPCGLAELCERTGLPRATAYRL
                     AAALEVHRLLARDDEGRWWLGPAVTELAAHVKDPLLAASATVLPALRETTGESVQLYR
                     REGTSRVCVAAVEPAAGLRDTVPVGARLPMTAGSGAKVLLAYSDAAIQKAVLPSAKFT
                     DRVLAEVRRRGWAQSVAEREPGVASVSAPVRDARGVVIAAISGSGPIDRIGRRPGARW
                     AADLLSAAEALTRRL"
     gene            10176..10688
                     /locus_tag="VFI55_07650"
     CDS             10176..10688
                     /locus_tag="VFI55_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET7073939.1"
                     /translation="MGSNQRASIVMSADEIADFVVKSRTGTLATVGPDGQPHLTAMWY
                     AVVDSEIWLETKAKSQKAVNLTRDPRVSFLIEDGDTYDTLRGVSFEGVAEIIDDPDVS
                     HRVGVSVWERYTGPYTEDMKPFVEQMMNKRVCVRIVARRSRSWDHRKLGLPAMPVGGS
                     TAPAALGTGQ"
     gene            complement(10719..10791)
                     /locus_tag="VFI55_07655"
     tRNA            complement(10719..10791)
                     /locus_tag="VFI55_07655"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10755..10757),aa:Glu,seq:ctc)
     gene            complement(10865..10936)
                     /locus_tag="VFI55_07660"
     tRNA            complement(10865..10936)
                     /locus_tag="VFI55_07660"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10901..10903),aa:Gln,seq:ctg)
     gene            complement(11040..12506)
                     /gene="gltX"
                     /locus_tag="VFI55_07665"
     CDS             complement(11040..12506)
                     /gene="gltX"
                     /locus_tag="VFI55_07665"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET7073940.1"
                     /translation="MTASVPVRVRFCPSPTGTPHVGMVRTALFNWAYARHTGGTFVFR
                     IEDTDAQRDTEESYLALLDALRWLGLDWDEGPEVGGPYGPYRQSQRTQIYREVVATLL
                     AAGEAYHAFSTPEEVEARHIAAGRNPKLGYDNFDRDLTEAQRAAFLSEGRRPVVRLRM
                     PDEGLSWDDLVRGTTTFAAGTVPDFALTRASGDPLYTLVNPCDDALMKITHVLRGEDL
                     LPSTPRQLALYQAMIRIGVAERIPEFAHLPTVLGEGTKKLSKRDPQSNLFAHRDRGFI
                     PEGLLNYLALLGWAIADDHDLFSLDEMVAAFDVVDVNSNPARFDQKKADALNAEHIRM
                     LGADDFAGRLRDYFSAHGHRIELDDAGFAAAAELVQTRIVVLGDAWDLLKFLNDDEYA
                     IDPKAAAKELGPDGIAVLDAALPALDGVVDWTTAEIEAALKAALIDGLALKPRKAFGP
                     IRVAATGTTISPPLFESLELLGRDRSVARLRAARDGAA"
     gene            complement(12503..13309)
                     /locus_tag="VFI55_07670"
     CDS             complement(12503..13309)
                     /locus_tag="VFI55_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET7073941.1"
                     /translation="MRLGRIASPDGVAFVSIDGELDNPAGMLAREIAEHPFGTPTFTG
                     RSWPLADVRLLAPMLAGKVVCIGKNYTDHIAEMSGLTGPAPADPVMFLKPNTAIIGPN
                     VPIRLPANASPVHFEGELAAVISRPCKDVPAARAADNILGYTIANDVSARDQQKADGQ
                     WTRAKGHDTFCPVGPWIVTDLSPLDPADLELRTEVNGEVKQHSRTSLMIHDVGAIVEW
                     ISAVMTLLPGDLILTGTPAGVGPIEDGDTVSITVQGIGTLTNPVVRKGKS"
     gene            13404..14642
                     /locus_tag="VFI55_07675"
     CDS             13404..14642
                     /locus_tag="VFI55_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7073942.1"
                     /translation="MPEGRPAVLSAGARWTVMAVSLFVTASSFLFINGIAFLIPALEA
                     NRGTPLAEAGLLSSMPSWGMVATLILWGYVLDRVGERLVLTLGSGLTAAAAYGAASAH
                     SMVSVGVFLFLGGMAAASCNTAGARLVSAWFPTHQRGLAMGIRQTAQPLGIALGALVM
                     PELAERGLRAGLMFPAFACGLAALASAVGIVDPPRKSRKAATADELASPYRGSTVLWR
                     IHAVAGLLMMPQTVTVTFMLVWLMDDHGWSVAAAGALVTLTQLLGAVGRIAVGRWSDR
                     IGSRMRPVRVIATAATSTLFLLAVADHLNTRYEVPLMVAISVIAVLDNGLEATAITEF
                     AGPFWSGRALGIQNTTQRVMAATGPPLFGALIAAARYPTAWALCGLFPLLAIPLVPTR
                     LVPPGLEARAAGPAEASSPG"
     gene            complement(14697..15707)
                     /locus_tag="VFI55_07680"
     CDS             complement(14697..15707)
                     /locus_tag="VFI55_07680"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET7073943.1"
                     /translation="MKLAIVGGDGIGPEVVAEAVKVLDAVVPGVQKTNYDLGARRFHA
                     TGELLPDSVLAELRQHDAILLGAVGDPSVPSGVLERGLLLRLRFELDHYVNLRPGRLY
                     PGVSSPLAGNPDIDFVVVREGTEGPYTGNGGAIRAGTPNEVATEVSVNTAFGVRRVVQ
                     YAFERARLRRKHLTLVHKNNVLTFAGKLWTRIVAEVGAEYPDVAVGYQHVDAATIFMV
                     TDPGRFDVIVTDNLFGDIITDLSAAVCGGIGLAASGNIDASRANPSMFEPVHGSAPDI
                     AGQGIADPIAAIMSVALLLAHLGEDDAAARVDRAVEAYLATRGNERLATSEVGDRIAA
                     AV"
     gene            complement(15742..17328)
                     /gene="serA"
                     /locus_tag="VFI55_07685"
     CDS             complement(15742..17328)
                     /gene="serA"
                     /locus_tag="VFI55_07685"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HET7073944.1"
                     /translation="MNLPVVLIADKLAESTVAALGDQVEVRWVDGPDREKLLAAVPEA
                     DALLVRSATTVDAEVLAAAPKLKIVARAGVGLDNVDVDAATERGVLVVNAPTSNIHSA
                     AEHAIALLLAASRQIAAADATLREHTWKRSSFNGTEIFGKTVGVVGLGRIGQLVAQRL
                     TAFGTHIVAYDPYVSAARAAQLGIELLPLDDLLARADFISVHLPKTSETAGLIDKEAL
                     AKTKPGVIIVNAARGGLVDEAALAEAVGSGHVRAAGLDVFSAEPCTDSPLFELPQVVV
                     TPHLGASTAEAQDRAGTDVAESVRLALAGEFVPDAVNVGGGVVNEEVAPWLDLARKLG
                     VLVAALSDGLPASLSVQVRGELAAEEVEVLRLSALRGVFSAVIEDQVTFVNAPALAAE
                     RGVTAEISKASESPNHRSLVDVRAVGHDGTTVNVAGTLSGPQLVEKIVQINGRNFDLR
                     ATGTNLVINYIDQPGALGKIGTLLGSAGVNIQAAQLSEDAAGPGATILLRLDQDVPND
                     VRTAIAEAVGANKLEVVDLS"
     gene            17359..18813
                     /locus_tag="VFI55_07690"
     CDS             17359..18813
                     /locus_tag="VFI55_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7073945.1"
                     /translation="MDVTFDVTIVGSGPNGLSTAVICARAGLKVQVVEAQPTFGGGAR
                     TAADFEFPGVLHDVCSAVHPLALASPFFAEFDLAARGVRMVVPEIAYANPLPGRPAAI
                     AYHDLERTCAELEHGASWRHLLGPLVEDPGAVIGLMLGDKRSVPTSLPAAARTAIRLG
                     LRTLAQGTPAWGTLAGDDARALFTGVAAHVISRLPSLTAAAAGLMLATLAHSAGWPIP
                     VGGSQAITDALIADLRAHGGEMTAGTEITGPPGGVVAFDTAPTALLRIYGDALPARYV
                     KALRRYRFGPGVAKVDFVLSDEIPWSDPRLRRAPTLHLGGTREQMARAEAEVAAGRHA
                     DWPMVLAASPHIADPGRTDSRGRRPFWTYAHMPAGSTVDATEAVTAVVERFAPGFRDV
                     VVAARAVPAARMADHDANYVGGDIGMGANSAWRAIAGPTPRLNPWRTPIPRVYLCSAA
                     TPPGGGVHGMSGYYAARTLLRREFGISDVPTMAP"
     gene            18810..19409
                     /gene="wrbA"
                     /locus_tag="VFI55_07695"
     CDS             18810..19409
                     /gene="wrbA"
                     /locus_tag="VFI55_07695"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HET7073946.1"
                     /translation="MTKLAIIYYSATGHGTAMAQRVAAAAESAGAEVRLRHVAETHDP
                     ESFAANPAWTANYEATKDLPAATGDDIVWADAVIFGSPTRFGSPAAQLRTFLDSLGGL
                     WAEGKLADKVYAGFTSSNTLHGGQETTLLSLYITLIHFGGIIVPPGYTDPCKFVDGNP
                     YGVSLVATHDNIHEFDEPTKNALEHLARRVVATADRLTS"
     gene            complement(19452..20453)
                     /gene="ilvC"
                     /locus_tag="VFI55_07700"
     CDS             complement(19452..20453)
                     /gene="ilvC"
                     /locus_tag="VFI55_07700"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET7073947.1"
                     /translation="MFYDDDADLSIIQGRKVGVIGYGSQGHAHSLSLRDSGVPVRVGL
                     KEGSKSRPKVEEQGLDVDTPAEVAKWADVIMLLAPDTAQADIFKNEIEPNLKPGDALF
                     FGHGLNIHFGLIKPPADVTVAMVAPKGPGHLVRRQFVDGKGVPALIAVDQDPTGNGEA
                     LALSYAKAIGGTRAGVIKTTFKDETETDLFGEQAVLCGGTEELVKAGFDVMVEAGYPP
                     EMAYFEVLHELKLIVDLMYEGGIARMNYSVSDTAEFGGYLSGPRVIDADTKERMRTIL
                     REIQDGTFVKKLVANIDGGNKQLEGLRKENAEHPIEVTGKKLRDLMSWVDRPITETA"
     gene            complement(20502..21023)
                     /gene="ilvN"
                     /locus_tag="VFI55_07705"
     CDS             complement(20502..21023)
                     /gene="ilvN"
                     /locus_tag="VFI55_07705"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET7073948.1"
                     /translation="MSNGHAPRTHTLSVLVEDKPGVLARVAALFSRRGFNIESLAVGA
                     TEQKDMSRMTIVVSAEETPLEQITKQLNKLINVIKIIEQDDGNSVSRELALIKVRADA
                     GTRSQVIEAVNLFRAKVIDVSPDSLTIEATGDRGKIEALMRVLEPFGIREIAQSGMVS
                     LSRGPRGIGTVKS"
     gene            complement(21023..22897)
                     /locus_tag="VFI55_07710"
     CDS             complement(21023..22897)
                     /locus_tag="VFI55_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HET7073949.1"
                     /translation="MSAPTRPHTPQTQRAADVASDAVKPAAKTPAVQPKHVAPEQLTG
                     AQSVIRSLEELDVEVIFGIPGGAVLPVYDPLYDSKKLRHVLVRHEQGAGHAASGYAHV
                     TGKVGVCMATSGPGATNLVTPLADAQMDSIPVVAITGQVGRGLIGTDAFQEADISGIT
                     MPITKHNFLVRSGDEIPRVLAEAFYIASSGRPGAVLVDIPKDVLQAQCTFSWPPQLDL
                     PGYKPNTKPHNRQVREAAKLIAAARKPVLYVGGGVIRGEATEELRELAELTGIPVVTT
                     LMARGAFPDSHPQNLGMPGMHGTVAAVGALQRSDLLIALGTRFDDRVTGKLDSFAPDA
                     KVIHADIDPAEIGKNRHADVPIVGDVKAVLTELIAMLRHGGAGASATPDMADWWAYLE
                     SVRATYPLSYGPQSDGSLSPEYVIEKLGEIAGPDAVYVAGVGQHQMWAAQFISYEKPR
                     TWLNSGGLGTMGFAIPAAMGAKIAHPDAEVWAVDGDGCFQMTNQELATCAIEGAPIKV
                     ALINNGNLGMVRQWQSLFYQERYSQTDLSTHSHRIPDFVKLAEALGCVGLRCEREEDV
                     VDVINQARAINDRPVVIDFIVGADAQVWPMVAAGTSNDEIQAARGIRPLFDDESEGHA
                     "
     gene            23246..23623
                     /locus_tag="VFI55_07715"
     CDS             23246..23623
                     /locus_tag="VFI55_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003900612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET7073950.1"
                     /translation="MVTDSPVVIRVSPMAHLAVGFLALGLLIPVLAWPPSAPLLLIPV
                     LLSALIVRLRTVADHDGVTVRTLLGSRTVPWDDIDGLRFHRGSWARARLKSGGELRLP
                     AVTFATLPLLTEASLGRVPNPYR"
     gene            complement(23627..24472)
                     /locus_tag="VFI55_07720"
     CDS             complement(23627..24472)
                     /locus_tag="VFI55_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7073951.1"
                     /translation="MTSQSHDAHWRRPGESPEPIPGRPASARLVDPEDDLTPVGYPGD
                     FGTTTVIPYSDPAASGGPAAAGYSLLDQQEPLPYVQPQSVAPAAAQPTEIDGDDDNDK
                     LRVAGRRGTQHLGLLVLRVWLGVVLCAHGLQKLFGWWGGQGVTGFKNSLSDVGYQHAD
                     ILAYVSVGGEILAGVLLVIGLFTPVAAAGALAFLINGLLASVSGRPHSHSFPFFLPDG
                     HEYQITLIVMAVAVVLCGPGRYGLDARRGWALRPFIGSFVALLLGIAAGIAVWVLLNG
                     DNPIA"
     gene            24733..>25781
                     /locus_tag="VFI55_07725"
     CDS             24733..>25781
                     /locus_tag="VFI55_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HET7073952.1"
                     /translation="MRLGRSVRGGLAALCAAVLVSSGCARFNDAQSQPFTTNPELRPQ
                     PSSTPPPPPPLPPTPFPKACPAPGVMQGCLESTSGLIMGTDSKTALVAERTTGAVKEI
                     SVSAEPKVKMVIPVDPSGDGGLMDIVLSPTYNQDRLMYAYISTPTDNRVIRIADGDIP
                     KDILTGIPKGATRNTGALIFTSPTTLLVMTGDAGNPAMAADPKSLAGKVLRIEQPTTI
                     GQAPPTTALSGVGSGGGMCVDPVDGSLYVADRTPTADRLQRITKTSEVSTVWTWPDKP
                     GVAGCAAMDGTVLVNLINTKLTVAVRLAPSTGAVTGEPDVVRKDTHAHAWALRMSPDG
                     NVWGATVNKTAGDAE"
BASE COUNT         4154 a         8778 c         8520 g         4329 t
ORIGIN      
        1 aaatcgaccc gcgcgaggcc ttggcagtca atggccttga acgcccggat cgccaactgg
       61 cgcacggcgt cagcgatgtc gtcgtcgacc tttgcgggca cgtccaattc ggctgcgtca
      121 tcgaggtatt tggtcgcgaa gtcgtagaac gcatcctcgc gcccccgcac tcctgccacc
      181 cggatctcgc ccaccgtgct ggcttccagt gtgccgtcgg gcatttcgag cactccgcac
      241 tcgagttcgc gaccgttgac cgccgcctcg atgatcactt tcgggtcgtg ccggcgggcg
      301 tgcgcaaccg cgccgggcag ctggtcccag ctcgagaccc ggctgacgcc aatcgaggag
      361 ccgccccgcg cgggtttgac gaacaccggc agacccagcc gttcgcactc ctcgggacgc
      421 aacgccgccc gcacggggcg cagcacgacg tgtgggccga ccggaagtcc ttcggcgaca
      481 agcagcttct tggtgaactc cttgtccatg cccgcggcac tggccagcac acccgcgccg
      541 acataaggca cgccggcgag ttcgagcagc ccctggatgg tgccgtcctc gccgtacggg
      601 ccatgcagca ccggaaacac cacgtccacg gacgccagga cttctcctgc tgcgggaccg
      661 gccgcgggcg agagcgacac caactggccg ccgcgccgcg gatccgccgg cagcgccagc
      721 tcggtgccgg attcggcggt caccccgggc agctgccggt tggtgatcgc cagcgcatcc
      781 gggtccccgt cggtgagcac ccacgaaccc tccggggtga tcccgatcgc gatcacgtcg
      841 aagcgctgcg ggtcgagatt gcgcagaatg ctgcccgcgg acacgcagga gatggcgtgc
      901 tcgttgctgc gcccgccgaa gacgacggcc acgcgcaccc ggcccgcggg tgacggccgt
      961 ggcgtcggaa accggtcgct tacattcaca acctaaagag gctaccggct ggagcgctcc
     1021 ggggtcgggt gccggaccgc tagctgggct ttccgtcggc gtggggcctc attcgggctt
     1081 ggtgctgcgg cccagcagca gcgccattgc ctcgtccacc gacaggcccc tgtgacagac
     1141 ccggtgcacg gcgtcggtga gcggcatttc gacgtcgtaa ctggacgcca gggcgagcac
     1201 cgcctcgcag gacgtcaccc cctcgacgac gtgggagtcc ctcgcctcca tcgccgactc
     1261 catcgtcccg ccgcggccca ggcactcgcc gaacgaccgg ttgcgcgact gcggggacgt
     1321 acacgtggcc accaggtcac ccacgcccgc cagcccggcc agcgtcgcgc ctttggcgcc
     1381 gagcgcgatg cccagccgga tgatctccgc caggccgcgg gtgatgatcg ccgccgcggt
     1441 gttttcaccc aggccgacgc cggcggccat tccgcacgcg agcgcgatga cgttcttgca
     1501 cgccccaccg acctcggtgc cgaccacgtc gctgttggtg taggggcgga agtacccgct
     1561 gttcagcatg cgctgcagcg ctaccgcgcg gccggagtcg ctgctcgcga cgacggtggc
     1621 ggagggctga cattgggcga tctcactggc caggttcggc cccgaaatga ccgcgacctg
     1681 cgtcgggtcc acgccggtga ccgacacgat gacctgactc atccgcatca gggtgcccag
     1741 ctcgataccc ttggccagac tgaccagggt cgcgccggcg gccagcgacg gtgcccaccg
     1801 ctcgagattg ctgcgcatcg tctgggccgg cactccgagc agcaccgtcg acgcgccggc
     1861 cagtgcctcg tcggcatcgg cggtggcacg gatgcccggc ggcagcaacg taccgggcag
     1921 gtaggcgggg ttgtaacggg tcgcgttgat ctgctcggcg acgctggatc gccgggccca
     1981 cagcgtgacc tcggcttctg gtcccccggc ctcggcgagc accttggcca gcgccgtgcc
     2041 ccaggcaccg gcacccatca ccgcaacggc gcccgatctg ctgcccatct accacccccg
     2101 tccgtcgtcc tgatgtgcca gcgcggcatc agcagccgct acaccctaga ccagcggcgg
     2161 gccgccgtca cgctagctca cgagcgacgc ggcgggcgtc gctggcagga tgacctcatg
     2221 agcggcagac gcgccgacgg tgcgggtgac ggtgcaggtg accggggaga cgtagccctg
     2281 atcatcgccg tcaagcggtt ggccgccgcc aagaccaggc tggccccggt gttctcggcg
     2341 ccgacccgtg aaagtgtggt gctggccatg ctggtcgaca ccctgaccgc cgcggcgcgc
     2401 gtcccggcgc tgggctcgat caccgtcatc acgcccgacg aggcggcggc cgccgcggcg
     2461 gccgagctcg gagccggggt gctggccgac ccgacggcca cgggccacgg cgacccgctg
     2521 aacaatgcga ttgccgccgc ggaacaagca gtggccgggg ccttcgccaa tgtcgttgcg
     2581 ctgcaaggcg atttgccggc actgcagagc caggagctgg ccgaggcgat cgccgccgca
     2641 cgcaaccacc ggcgcagctt cgtcgccgac cggctggcca ccgggaccgc cgcgctgtgt
     2701 gcgttcggaa ccccgctcga tccgcggttc gggccggatt cggccgcgcg gcaccgccgt
     2761 tcgggcgcga tcgagctgac gggcgcgtgg cccggcctgc gctgcgacgt cgacaccccc
     2821 gccgacctgg cggccgcccg tcgtctcggg gtcggggcgg cgaccgctcg ggccatcacg
     2881 cagcactagg cacacctggc aaagcgacaa acctgaccga acggttaacg gcgcaccaac
     2941 ggcgcgtgga tctccgccac gcgctggcac cagccgcggg tgatgagcaa tgatcgcatg
     3001 gtgactgaaa tcgaagctga agcccggccc gacgagaact tatggcattc aggcgagtcg
     3061 gccgtcgcgg caccgcctgc ggccaccccc gccactagcg ctgacgagct tcccgaggac
     3121 cgctacctca accgggaact gagctggctg gatttcaatg cgcgcgtgtt ggcactggcc
     3181 gccgacagct cgctgccact gctggagcga gccaaattcc tggcgatctt cgcgtccaac
     3241 ctcgacgagt tctacatggt gcgggtcgcc ggcctgaagc gccgcgacga gatggggcta
     3301 tcggtgcgct ccgccgacgg cctgacgccg cgtgagcaac tgagacgcat cggcgagcag
     3361 acccagcaga tcgccagtcg gcacgcccgg gtgttcctcg attcggtgcg gccggcgttg
     3421 gccgaggaag gcatccacat cgtcacgtgg gccgatctgg acaaggctga gcgcgagcaa
     3481 ctatcgacct atttcaccga gcaggttttc cccgtcttga cgccgttggc cgtcgacccc
     3541 gctcacccgt tcccgttcgt ttccgggttg agcctgaatc tggccgtcac cgtacgccaa
     3601 cccgaggacg gcggccagca tttcgctcgg gtcaaggtgc ccgacaacgt caatcgcttc
     3661 gtcgaactcg ctgtccgcga ggcgggaaaa gagaactccg ccggaaccga gggaaagacc
     3721 gtaatccgtt atctgccgct ggaagaactc attgcggcgt ttcttcccgt gcttttcccg
     3781 ggcatggaga tcgtcgagca tcacgcgttc cgcatcaccc gcaacgccga tttcgaggtt
     3841 gaagaggacc gcgacgaaga cctgctgcaa gcgctggagc gggaattggc gcgccggcgc
     3901 ttcggttccc cggtgcgact cgagatcgcc gacgacatga ccgagagcat gctcgaattg
     3961 ctgttgcgcg aactcgacgt gcatcccggt gacgtgattg aagtgcccgg gcttttggac
     4021 ctgtcgtcgt tgtggcagat ctacgggctg gaccgcccgg cgctgaagga cgcggctttc
     4081 gttccggcca ccaatcccgc cttcgccgac cgggaaacgc cgaagagcat cttcgccacg
     4141 ctgcgcgaag gtgatgtgct ggttcatcat ccatatgatt cgttctccac cagcgtgcag
     4201 cgattcatcg agctggccgc cgcggacccc aacgtgctgg cgatcaaaca gacgctctac
     4261 cgcacctccg gtgactcgcc catcgtccgg gcgctcatcg ccgccgccga agccgggaag
     4321 caggtggtgg caatggtcga gatcaaggcg cgctttgacg aacaggccaa catccgctgg
     4381 gcgcgcacac ttgagcaggc gggcgtgcac gtggtctacg ggtacgtcgg gctgaagaca
     4441 cactgcaaga cctgcctggt ggtgcgtcgc gagggttcga cgatccggcg gtactgccat
     4501 gtcgggaccg gcaactacaa cggcaagacg gcgcggctgt acgaggacct cggtctgctc
     4561 acggcgtctc ccgaaatcgg tgcagatctc accgatttgt tcaattcgct taccggctac
     4621 tcgcgcaagg tgtcctaccg caacctgttg gtggccccac acagcattcg cgcgggcatc
     4681 atcgaacgcg tcgagcgcga ggtcgccgcg caccgtgaaa gcggtgacgg gcacatccag
     4741 ctcaagatga actcgctcgt cgacgagcag gtcatcgatg cgctgtatcg cgcgtcccag
     4801 gccggcgtgc gtgtcgagat agtggtgcgc ggcatctgcg cactgcgccc gggtgcggag
     4861 ggcttctccg aaaacgtcct cgtccgatcg attctcggcc gattcctgga gcactcgcgg
     4921 atcatccgct tcggtcggat caacgaattc tggatcggca gcgccgacat gatgcaccgc
     4981 aatctcgacc ggcgcgtcga ggtgctggtt caggtcaagg acccgaggct caccacatat
     5041 ctgaacgaat tgttcgagtc ggcactggac ccgtccacgc gatgctggga gctgggatcc
     5101 gacgggcagt ggaccgcgtc accccaggcg ggtcacaccg tgcgcgacca ccaagtatgg
     5161 ttgatggagc ggcacaccag cccctaagac ccagccccta acacaccctc taagacaccg
     5221 tgtggtgatc tacaggccac atccacggcc gtgcctggca tggccgaatt gacctgcagg
     5281 agttgagttg tcgacccaga attcgtcggc cggtcggcgc tcggggagcc ggatcgtgta
     5341 cgccgcgggt gcggtgttgt ggcggccggc cggtgccggt ccggccaacg cggacctcga
     5401 gatcgctgtg atccaccgcc ctcgctacga cgactggtcg ctgcccaagg gcaaggtgga
     5461 cccgggcgag acggcgccgg tggcggcggt gcgggaagta ttcgaagaga cgggtcacca
     5521 cgccatcctc ggcaggcgcc ttgacttggt gagctatccc atcgagcagg gcatcaagaa
     5581 ggtctattac tgggcggcgc ggagcatcgg cggtgaattc acgccaggcc gcgaggtcga
     5641 cgaattggtg tggttgccgg tcgccgacgc gatgccgaaa ctgaactacg cgcaagatcg
     5701 aaagatgttg cagcacttca cgaaaaagcc cgcagacacc cacaccgtgc tcatcgtccg
     5761 gcacggcacc gcgggccaaa aatcgcgttt ctctggcgac gacaccaaac gaccgctgga
     5821 caagagggga cgcgcgcagg cggaggcgct ggttccgcag ctgctggcat tcggtgcttc
     5881 cgacgtgtac gcggccgacc ggctgcgctg tcagcaaacg gtagaaccgc tcgccgagga
     5941 actgggtgtg gctgtccacg acgagcccac gctgaccgag gagtcctacg ccaagagccc
     6001 caagcgcggc cgcaaccgga tgctgaaaat cgccgagcag gacggcacac cggcgatctg
     6061 cacgcagggc aaggtaattc cggacttgat cgcgtggtgg tgcgaacggg acggagtacg
     6121 tcccgacaaa tcccgcaacc acaagggcag cacctgggtg ctgtcactgt cggcggggcg
     6181 actggtcgcg gccgaccaca tcggcggcgc gctggccgcc aacgtgcggg cctaacacgc
     6241 gaataccctt cgggtggcat cgctgccacc gcgaagggta tctgcgggtg ctacttacta
     6301 attgctactt acgaccgcgc cgcggagtcg ccttcttggc aggcgccttg ctggccggcc
     6361 tctttgccgc gaccttcttg gccggtgcct tgctggcgga cttcgtcacg gccttcttga
     6421 ccggagcctt ggcggcggcc ttcttgaccg gagccttgac ggcggccttc ttgaccggag
     6481 ccttcgtcac agccttcttg accggagcct tcgtcacagc cttcttggcg ggggccttgg
     6541 tcgcagcctt cttggccgga gccctcgtcg cggtcttctt agcgggcgcc ttcttggccg
     6601 ccttcttggc cgccacgcct gctaccacac cgcgcttgac tgcggggcct tccgccggga
     6661 ggcgctgtgc gccagacaca accgctttga attgggcacc ggggcggaag gccgggaccg
     6721 acgtgggctt cacctttacc gtctcgccgg tgcgcggatt gcgggccacc cgcgccgcgc
     6781 ggcgccgctg ttcgaacaca ccgaacccgg taatggttac gctgtcgccc ttatgcaccg
     6841 cacgcacaat ggtgtcgacg aaattctcga cggcggcggt cgcctgccga cggtccgagc
     6901 ccaatttctg tgtgagcacg tcaatgagct ctgctttgtt catccaaccc tccgaagcta
     6961 gtagtcctcg atttggaacc gactagtgga cacggtaaac cctcacccag caaatttcca
     7021 agagccacgc gcaatttcag gacccggcgg ccgaggtttt cgcaatccgg ttgccgccct
     7081 tgaccgatgg cggggccgga aaatcggccg ggtttgcttg gccggacggg aagaaaatcc
     7141 gctccggccg accgttcagg aggcgggcaa agtgcgcggt ttccactccg gataggtcgc
     7201 ctcgaattcc tcgattttgt cgagtttccg cagcgtaagg gctatatcgt caagaccttc
     7261 gagcagtctc caagcggtat agtcgtcaat cttgaacggc agcaccgtcg ttccggcggt
     7321 gatatttcga tcttgaagat tggcagtgat ttccaagccc ggattctgct cgatgagctt
     7381 ccaaaggagc tcgacgccgt cttgggaaac ttcggccgcc aggagacctg ctttaccgga
     7441 gttgccccga aaaatgtcac cgaatcgaga tgagatgacc acccggaacc catagtccat
     7501 gagcgcccac accgcgtgct cgcgggatga tccggtgccg aaatcgggtc cggcgaccag
     7561 aaccgagccg cggtcaaagg ggctgaggtt gagcacgaat gagggatccg accgccatgt
     7621 cgcgaacaag ccgtcctcga aaccggttcg ggtgacgcgc ttcaaaaaca ccgcgggaat
     7681 gatctgatcg gtatccacat tggaccgccg cagcggcact ccgataccgg tgtgggtgcg
     7741 aaagggttcc atcgtcgtcc tcgaatccgt tcggtcagtt tgagttcagg tcggccggcg
     7801 tagacagcct gccccgcacc gctgttgcgg cggcgacgac gggcgacacc agatgggtgc
     7861 ggccgccttt gccctgccgc ccttcgaagt tgcggttgga cgtcgacgcg caccgctgcc
     7921 ccggggagag ctgatcgggg ttcatgccca ggcacatcga gcatcccgcc tgccgccatt
     7981 cggcgcccgc ggcggtgaaa acctcgccca gcccttcgtc ttcggcctgc gcgcgcaccc
     8041 gcatagagcc gggaacgacg agcatccgca ccccctcggc caccttgcgg ccgcgcaaca
     8101 catcggccac cacccgcaga tcttcgatac gaccattggt acaagagccg acgaacacgg
     8161 tgtccacgct gatatcgcgc atcggcgtcc cgggccgaag gtccatgtac gccaaggctt
     8221 tctcggcggc ttggcgctcg ccatcgtcgg tcatcagttc ggggtccggc accgccgcgt
     8281 ccaacggcac cccctgcccg gggttcgtcc cccaggtgac aaatggactc aacgaggccg
     8341 catcgagata gacctcggtg tcgaagacgg cgccggggtc ggtgcccaac tgctgccagt
     8401 actcgagtgc tgcatcccat tgcgcgccgg tcggcgcgtg cgggcggccg cgcaggaatt
     8461 cgtaagtggt cgcgtccggc gccaccatgc ccgcccgggc gccggcttcg atgctcatgt
     8521 tgcagatcgt catccggcct tccatcgaca gcgattcgat ggcgctgccc cggtattcga
     8581 tgacatggcc ctgcccaccg ccggtgccga tcttggcgat caacgtaaga atgatgtcct
     8641 tggcggtcac tccggcgggc aatgtcccgt ccacgttgac cgccatcgtc ttgaatggcc
     8701 gcagcggcaa cgtctgagtg gccatcacgt gctcgacctc cgaggtgccg atgcccatcg
     8761 ccagcgcgcc aaacgcgcca tgggtcgacg tgtgactgtc gccgcagacg atcgtcattc
     8821 ccggttgggt gaggcccaat tgcggtccga cgacatgcac gatgccctgt tcgatgtttc
     8881 ccatcgggaa tagccggata ccgaattcgg cgcagttgcg ccgcaacgtc tcgacctgag
     8941 tgcgcgacac caggtcggcg atcagattgt caacgccggt cgtgggaacg ttgtgatcct
     9001 cggtggccag cgtcaaatcg gggcggcgca ccggccggcc ggccaggcgc agcccgtcga
     9061 aggcctgcgg gctggtgacc tcgtgcacca gatgcaggtc gatgtagatc aagtccggcg
     9121 cactggcctc gtcgcccgac gagccagaca cgacaacgtg gtcttcccaa accttttcgg
     9181 ccagggtgcg cggtttggcc gggccgtcct tgccggcagc cggggaaatg tcggatccca
     9241 tgtcgaactc gcctcgattc acctcatccg cggctcgccg atcgacttcg gactgttgat
     9301 ctcagcatgc gagacgctag tatctctttg tgagacagca tagcggcatc ggcgtactcg
     9361 acaaagcggt gggcgtgctg catacgatcg cggaatctcc ctgcgggctg gccgaactct
     9421 gtgagcgcac cggattgccc agggccacgg cctaccggct ggcggcggct ctggaagttc
     9481 atcggctgct ggcgcgcgac gacgaagggc gttggtggct tggtcccgcg gtcacggaat
     9541 tggcggccca tgtcaaggat ccgctgctgg ccgcgagcgc gacggtgcta cccgccttgc
     9601 gtgagaccac gggcgaaagc gtgcagctgt accgccgcga ggggacgtcg cgggtgtgcg
     9661 tggccgccgt ggaacctgct gcgggtctgc gcgatacggt tccggtcggg gcgcggttgc
     9721 cgatgacggc gggctcgggg gccaaagtgc tactcgccta cagcgacgcc gccatccaga
     9781 aagccgtgct gccgtcggcg aaattcaccg accgggtgct ggcagaggtg cgccggcgtg
     9841 gttgggcgca aagcgtggcg gaacgcgagc cgggggtggc cagtgtgtcc gcacccgtcc
     9901 gcgacgcccg cggtgtcgtc atcgccgcca tatcggggtc cgggcccatc gaccggatcg
     9961 ggcgacgccc gggggcgcgc tgggccgccg atctactgtc ggcggcagaa gccctcaccc
    10021 gtcggctcta gagattcggc gccgccaggg cgcagtgtgc atcgttggcg tcgggtgtgt
    10081 atccagggcg gcagaatcgc gaattcctcg ccgtcagcgc acacggaaag tcgccagtgc
    10141 acactccccg cacccccgcc tgacacactg gcgccatggg aagcaatcag cgcgcgagca
    10201 tcgtcatgtc cgctgacgag atcgccgatt ttgtcgtcaa gagccgcacc gggacgctgg
    10261 ccaccgtcgg tcccgacggg cagccgcact tgaccgccat gtggtacgcc gtggtcgaca
    10321 gcgaaatctg gctggagacc aaggccaagt cgcagaaggc ggtcaacctg acgcgggacc
    10381 cgcgggtgag cttcctcatc gaggacggcg acacctatga cacgctgcgc ggggtgtcct
    10441 tcgaaggcgt cgcggagatc atcgacgatc ccgacgtgtc gcaccgggtc ggggtcagcg
    10501 tgtgggagcg ctacaccggc ccctacaccg aggacatgaa gcccttcgtc gagcagatga
    10561 tgaacaagcg ggtgtgcgtg cggatcgtgg ccaggcggtc acgctcgtgg gatcaccgca
    10621 agctcggatt gcccgccatg ccggtgggcg gatcgaccgc accggccgcg ctggggaccg
    10681 gccagtaagc acgcctggcg gcccggtaga cggttttgta gccccgatgg gattcgaacc
    10741 cacgctaccg ccgtgagagg gcggcgtcct aggccgctag acgacggggc cagaaccgat
    10801 ccgagctgac agcatagctc accccggtag gcgcacctaa tcgcgttgcg ctggtgcagc
    10861 tttgctgggg taccaggact cgaacctaga atggctgaac cagaatcagc tgtgttgcca
    10921 attacaccat accccattgg ctgcctaaaa ctgctggtca acggcctttt tggaccctag
    10981 ttagcatcca gagaccgtcg ccgtcagccg tgataagccc tgtgcacatt accaaagaat
    11041 tacgccgcgc cgtcgcgcgc ggcccgcagc cgcgccacgc tgcggtcgcg gccgagcagc
    11101 tccagcgatt cgaacaacgg cgggctgatc gtcgtcccgg tcgcggcaac cctgatcgga
    11161 ccgaacgcct tacgcggttt gagcgccaga ccgtcgatga gggcggcctt gagggccgcc
    11221 tcgatctcgg ccgtggtcca gtccaccacg ccgtccagcg cgggcagcgc cgcgtccagc
    11281 accgcgatcc cgtctgggcc gagctccttg gccgcggctt tggggtcgat cgcgtattcg
    11341 tcgtcgttga ggaacttcag caggtcccac gcgtcgccga gcacgacgat gcgggtctgc
    11401 accaactcgg cggcggcggc gaatccggcg tcatcgagct cgatgcgatg gccgtgcgcg
    11461 ctgaaatagt cgcgcagcct gccggcgaag tcgtccgcgc ccagcatccg gatgtgctcg
    11521 gcgttgagcg cgtcggcctt cttctggtcg aacctggccg ggttggagtt gacgtcgacg
    11581 acgtcgaacg cggccaccat ttcgtcgagg ctgaacaggt cgtgatcgtc ggcgatcgcc
    11641 cagccgagca acgcgaggta attcagcagc ccctcgggaa tgaagccccg gtcgcggtgg
    11701 gcgaacagat tcgactgcgg gtcgcgtttg gaaagcttct tggtcccctc ccccaacacc
    11761 gttgggagat gggcgaattc cggaatacgc tcggccaccc cgatccggat catcgcctga
    11821 tacagcgcca gctggcgcgg agtcgacggc agcaggtcct cgccacgcag cacatgggtg
    11881 atcttcatca gcgcgtcgtc gcacgggttc accaaggtgt acaacggatc tccgctcgcg
    11941 cgggtcagcg cgaaatcggg cacggtgccc gccgcgaagg tggtggtccc gcgcaccagg
    12001 tcgtcccaac tgaggccttc gtcgggcatc cgcagccgga ccaccggcct acggccttcg
    12061 gaaaggaagg ccgcgcgctg cgcttcggtc aggtcccggt cgaagttgtc gtagccgagc
    12121 ttggggttgc ggccggcggc aatgtgacgg gcctcgacct cctcgggggt tgagaaggcg
    12181 tggtaggcct ctccggccgc gagcagcgtg gccaccacct cacggtagat ctgggtgcgt
    12241 tgcgactgcc ggtacgggcc gtagggtccg ccgacctcgg gtccctcgtc ccagtcgagg
    12301 ccgagccagc gtagcgcgtc gagcagcgcc agatagctct cctcggtgtc gcgctgcgcg
    12361 tcggtgtcct cgatgcgaaa gacgaaggta ccgccggtgt gtcgggcgta cgcccagttg
    12421 aacagcgcgg tgcggaccat cccgacgtga ggggtgccgg tgggcgacgg gcagaatcgg
    12481 acccggacag ggactgatgc ggtcacgact ttcctttgcg gactacggga ttggtgaggg
    12541 tgccgattcc ctggacagtg atggagacgg tgtcgccgtc ctcgatgggt ccgacacccg
    12601 ccggtgttcc ggtgaggatg agatcgccag gcagcagggt catcacggcc gagatccact
    12661 ccacgatggc gccgacgtcg tggatcatca gcgaggtgcg gctgtgttgc ttcacttcgc
    12721 cattgacttc ggtgcggagt tcgagatccg ccgggtctaa aggactcagg tcggtgacga
    12781 tccacggccc gaccgggcag aacgtgtcgt ggcctttggc ccgggtccat tggccgtcgg
    12841 ccttttgctg atcgcgcgcc gacacgtcgt ttgcgatggt gtagccgaga atgttgtccg
    12901 cggcgcgggc ggccgggacg tccttgcacg gccggctgat caccgccgcc aactcgccct
    12961 cgaagtgcac gggtgatgcg ttggcgggca accggatcgg cacgttcggt ccgatgatcg
    13021 cggtgttggg cttgaggaac atcaccgggt ccgccggcgc agggcccgtc aggccgctca
    13081 tctcggcgat gtgatcggtg tagttcttac cgatgcagac caccttgccc gccagcatcg
    13141 gagccagcag ccgcacatcg gccagcggcc acgatcggcc ggtgaacgtg ggcgtgccga
    13201 aagggtgttc ggcgatctcc cgcgcgagca ttcccgctgg attgtcgagt tcgccatcga
    13261 tgctgacgaa ggcgacaccg tccgggctgg cgatacgacc aaggcgcatt cgcttcagcc
    13321 tactaatggt caactgcggc aggcgcagcc gggcgccggg ttggatcgtc aacgcccacc
    13381 atcgggtgtg gaagcatgga ctgatgcctg agggccgccc cgccgtgctc agcgcgggag
    13441 cgcgctggac tgtcatggcc gtgtcgctgt tcgtgacggc gagttcgttc ctcttcatca
    13501 acgggatcgc cttcctgatc cccgcgctcg aagccaaccg tggcaccccg ctggccgaag
    13561 ccggtctgtt gtcgtcgatg ccgagctggg gcatggtcgc cacgctgatc ctctggggct
    13621 acgtgctcga ccgcgtcggc gaacggctcg tgctgaccct gggctcggga ctcaccgccg
    13681 cagcggccta tggcgccgcg tcggcccatt cgatggtgtc ggtcggtgtc ttcctgtttc
    13741 tcggcggcat ggccgcggcg agttgcaaca ccgccggcgc ccgactggtg tccgcctggt
    13801 tcccgacaca ccaacgcggc ctggccatgg gcatccgcca gaccgcccag ccgctgggaa
    13861 tcgccctggg cgcgttggta atgcccgagc ttgccgaacg cggcctgcgc gcggggctga
    13921 tgtttccggc gttcgcgtgc gggctggccg ccctggccag tgcggtcggc atcgtcgatc
    13981 cgccgcgcaa atcccgcaag gccgccaccg cagacgaact ggccagcccg taccgcggct
    14041 cgaccgtcct gtggcggatc cacgcggtgg cgggcctgct gatgatgccg cagacggtga
    14101 ccgtgacctt catgttggtt tggctgatgg acgaccatgg ctggtccgtc gcggccgccg
    14161 gcgccttggt cacgctcaca cagttgctcg gtgccgtggg ccgcatcgcg gtgggccgct
    14221 ggtcggaccg gatcggctca cgaatgcggc cggtccgcgt cattgcgacc gcggctacct
    14281 caaccttgtt cctgctcgcc gtcgccgacc atctcaacac gcgatacgag gtaccgctga
    14341 tggtcgcgat ctcggtgatc gcggtgctcg acaacgggtt ggaggcgacg gcgatcaccg
    14401 aattcgccgg gcccttctgg agcggacgcg ccttggggat ccagaacacc acccagcggg
    14461 tgatggcggc caccggaccc ccgttgttcg gtgcgttgat cgcggcggcc agatacccga
    14521 cggcctgggc gctctgcggg ttgttcccgt tgctggcaat accgttggtg ccgacccggt
    14581 tggtgccacc cggcttggag gctagagccg ctggcccggc tgaagcgtcg tcgccaggct
    14641 gaagccgacg gcccggcttc tcctcagacg gctccgccgt ctgcatcgtc gccaggctag
    14701 accgcggctg caatccgatc gccgacttcg ctggtggcaa gccgttcatt cccacgagtt
    14761 gcgagatagg cctcgacggc ccggtccacc cgggcagcgg catcgtcctc gccgaggtgg
    14821 gcaagcaata gcgccaccga catgatcgcc gcgatcggat cggcgatgcc ctgaccggca
    14881 atgtcgggcg cgctgccgtg caccggctcg aacatcgacg gattggcccg cgaggcgtcg
    14941 atatttccgc tggcggccaa gccaattccg ccgcacacgg ctgccgacaa gtcggtgatg
    15001 atgtcgccga acaggttgtc ggtcacgatg acgtcgaagc gcccggggtc ggtgaccatg
    15061 aagatggtgg cggcgtcgac gtgttgatag ccgaccgcga cgtcgggata ttctgcgccc
    15121 acctcggcca cgatccgcgt ccacaacttc cccgcgaacg tcagcacgtt gttcttgtgc
    15181 accaacgtca ggtgtttgcg acgcagccgg gcccgctcga aggcgtactg caccactcgg
    15241 cgcacaccga acgcggtgtt cacgctgacc tcggtggcca cctcattggg cgttccggcg
    15301 cggatcgcac cgccgttgcc cgtgtacggc ccctccgtgc cttcgcgcac caccacgaaa
    15361 tcgatgtcgg ggtttccggc gagcgggctg ctcacccccg ggtacagccg gcccggacgc
    15421 aggttgacgt agtgatccag ctcaaagcgc aggcgcagca acagacctcg ctccagcacc
    15481 ccactgggca ctgacgggtc gccgaccgcc ccgagcagga tggcatcgtg ctgccgcaat
    15541 tcggcgagca ccgaatccgg cagtagctca cccgtggcgt ggaatcgacg ggcgcccaga
    15601 tcgtagttgg tcttctgcac gccgggcaca acggcgtcga ggactttgac cgcttcggca
    15661 accacctccg ggccgatccc gtcgccgccg acgatggcca acttcatcgt cggcttccca
    15721 ccgcagcgtc gccggagggg gtcacgacag atcaaccacc tcgagcttgt tggcgcccac
    15781 cgcctccgcg atcgccgtcc gcacgtcatt gggcacgtcc tggtccagcc gcagcagaat
    15841 cgtcgcgccc gggcccgcgg cgtcctcgga caactgcgcg gcctggatgt tcaccccggc
    15901 cgagcccagc aacgtgccga tcttgcccag cgcgcccggc tgatcgatgt agttgatcac
    15961 caagttcgtg ccggtcgccc gcaggtcgaa gttgcgccca ttgatctgca cgatcttctc
    16021 caccaattgc ggaccggaca gcgtgcccgc gacgttgacg gtggtgccgt cgtgcccgac
    16081 cgcccgcacg tcgacgaggc tgcggtggtt ggggctttcc gaggccttgg agatttcggc
    16141 ggtgacgccg cgctcggcag ccagcgcggg cgcgttgacg aatgtcacct ggtcttcgat
    16201 gaccgccgag aacacgccgc gaagcgccga aagcctcagc acctcaactt cttcggcagc
    16261 cagctcgcca cgcacctgca ccgacagcga tgccggcagg ccatcggaca gcgcggcgac
    16321 cagcaccccg agcttgcggg ccaggtccag ccacggcgcc acctcttcgt tgaccacacc
    16381 accgccgacg ttgacggcat cgggcacgaa ctcccccgcc agcgccaacc gcacgctctc
    16441 ggccacatcg gtgcccgccc ggtcctgagc ctcggccgtg gacgccccaa ggtgcggcgt
    16501 caccacgacc tggggcagct cgaacagcgg gctgtcggtg cacggctcgg ccgagaacac
    16561 gtcaagaccg gcggcccgca cgtgtccgct gcccaccgcc tcggccagcg ccgcctcgtc
    16621 caccagcccg ccgcgggcgg cgttgacgat gatgacgccc ggcttggtct tcgccagtgc
    16681 ctctttgtcg atcagaccag ccgtctcgga cgtcttcggc aaatgcacgg agatgaagtc
    16741 ggcgcgggcc agcaggtcgt ccaggggcag cagttcgata cccagctgag cggcccgggc
    16801 cgccgacacg tacgggtcgt aggccacgat gtgggtaccg aaggccgtca atcgctgggc
    16861 caccaactga ccgatccggc ccagcccgac gacacctacg gtcttgccga aaatctcggt
    16921 gccattgaac gacgaccgct tccaggtgtg ttcgcgcagc gtggcgtcgg ccgccgcgat
    16981 ctggcgggac gccgccagca gcagcgcgat cgcgtgctcg gcggcgctgt ggatgttcga
    17041 ggtgggcgcg ttgaccacca gcacaccgcg ttcggtggca gcgtccacgt cgacgttgtc
    17101 gagcccgacg ccggctcggg cgacgatttt cagtttggga gccgctgcca gcacttcggc
    17161 gtcgacggtg gtggccgaac gcaccagcag cgcgtcggcc tctggcaccg ctgccagcag
    17221 cttctcccgg tcgggaccgt ccacccagcg cacctcgacc tggtctccca gggcggcaac
    17281 ggtcgattca gcgagtttgt cggcgattaa cacaacaggc aagttcacgc cgacagcgta
    17341 gtcggctgta atcgggtgat ggacgtcacc ttcgacgtaa caatcgtcgg cagcgggccc
    17401 aacggacttt ccacggccgt gatctgcgcc cgcgcgggcc tgaaggtaca ggtcgtcgag
    17461 gcccagccga cgtttggcgg cggcgcgcgc accgcggccg acttcgaatt tcccggagtc
    17521 ttacacgacg tgtgctccgc ggtgcacccg ttggcgctgg cgtcgccgtt cttcgcggag
    17581 ttcgacctgg ccgcgcgcgg cgtgcgaatg gtggtaccgg agatcgccta tgccaacccg
    17641 ctgccgggcc ggcccgcggc gatcgcctat cacgacctcg agcgcacctg cgccgagttg
    17701 gagcacggtg cgtcctggcg acacctgctc ggccccttgg tcgaggaccc cggcgccgtc
    17761 atcggcctca tgctcggtga caaacgttcg gtgcccacct cgttgccggc ggcggcgcgc
    17821 accgcaatac ggttgggcct ccggacgctg gcccaaggaa cccccgcctg ggggacgctg
    17881 gccggtgacg acgcccgtgc cttgttcacc ggtgttgccg cccacgtgat ttcgcggttg
    17941 ccgtcgttga cggcggcggc tgcgggtttg atgttggcaa cgctggcgca ctcagccggc
    18001 tggccgattc cggtgggagg cagccaggcg atcaccgatg cgctgatcgc tgacctccgc
    18061 gcgcacggcg gcgagatgac ggccggcacc gaaatcaccg ggccgcccgg cggggtggtt
    18121 gcgtttgaca cggccccgac cgcgctgctg cggatctacg gtgacgccct tccggcgcga
    18181 tatgttaagg cgttgcgccg ctaccgattt gggcccggcg tggccaaggt cgacttcgtg
    18241 ctcagtgacg agattccgtg gtcggacccg cgcctgcggc gcgcgccgac ccttcatctc
    18301 ggcggcaccc gcgagcagat ggcgcgagcc gaggcggagg tggcagccgg gcgtcatgcc
    18361 gactggccga tggtgctggc cgcctcgccg cacatcgcgg atccggggag gacggatagc
    18421 cggggtcgcc gtccattctg gacgtatgcc catatgccgg cgggttccac cgtcgacgcg
    18481 acggaggcgg tgaccgcggt ggtcgagcgg tttgcgcccg gcttccgcga cgtcgtggtg
    18541 gcggcccgcg ccgtgcccgc cgcacggatg gccgaccacg acgccaacta cgtgggtggc
    18601 gacatcggca tgggcgcaaa ctccgcctgg cgtgcgatcg ccggccccac accccgcctg
    18661 aacccttggc gcacaccgat tcccagggtg tatctgtgct cggcggccac accgcccggc
    18721 ggcggcgtgc acggcatgtc cggttactac gccgcgcgga cgttgctgcg gcgtgaattc
    18781 ggaatcagcg atgtgcctac gatggccccg tgacgaaact cgcgatcatc tactattcgg
    18841 ccaccggtca cggcaccgcg atggcacagc gcgtcgccgc ggcggcggag tcggcgggcg
    18901 cagaagtccg tttgaggcat gtcgcagaaa cccacgaccc cgagtcgttc gccgccaacc
    18961 cggcgtggac ggccaactac gaggccacca aggatcttcc cgcggccacc ggcgacgaca
    19021 tcgtgtgggc cgacgcggtg atcttcggct cacccacccg gttcggctcg ccggcagcac
    19081 aattgcgcac cttcctggat tccctcggcg ggctgtgggc cgaaggcaag ctggccgaca
    19141 aggtgtacgc gggcttcacg tcgtccaaca ccctgcatgg cggccaggag accacgctgc
    19201 tctcgctgta catcacgctg atccatttcg gcggcatcat cgtgccgccg ggatacaccg
    19261 atccgtgcaa gttcgtcgac ggcaatccgt acggggtgag cctggtcgcc acccacgaca
    19321 acatccacga gttcgacgag cccaccaaaa acgcgctcga acacctggcc cgccgggtgg
    19381 tggcgacagc cgaccgcctc acgtcgtagt gacatcgacg aacaccccgg ctagccggag
    19441 ctggacccgg cctaggccgt ctcggtgatc gggcggtcga cccagctcat caggtcgcgc
    19501 agcttcttgc cggtgacctc gatgggatgc tcggcgtttt ccttgcgcag gccctcgagc
    19561 tgcttgttgc cgccgtcgat gttggcgacc agcttcttga cgaaggtgcc gtcctggatc
    19621 tcgcgcagga tggtgcgcat ccgctccttg gtgtcggcgt cgatgacgcg gggacccgac
    19681 aggtatccac cgaattccgc ggtgtcggac accgagtaat tcatccgcgc gatgccgccc
    19741 tcgtacatca ggtccacgat cagcttgagc tcgtgcagca cctcgaagta ggccatctcc
    19801 ggcggatagc ccgcctcgac catcacgtca aagccggcct tcaccaattc ctctgtgcca
    19861 ccgcacaaca cggcctgctc accgaacagg tcggtttcgg tctcgtcctt gaaggtggtc
    19921 ttgatgacgc ccgcccgggt gccgccgatg gccttggcat aggacagcgc cagcgcctcc
    19981 ccgttcccgg tcggatcctg gtcgacggcg atcagggcgg gcacgccctt gccgtcgacg
    20041 aactgtcggc gcaccaggtg gccgggcccc ttgggggcga ccatcgcgac ggtgacatcg
    20101 gccggcggct tgatcaggcc gaagtggatg ttgagtccgt gaccgaaaaa cagtgcgtca
    20161 ccgggcttca ggttgggctc gatctcgttc ttgaagatgt cggcctgggc ggtgtcgggc
    20221 gccagcagca tgatgacgtc ggcccatttg gcgacttcgg cgggggtgtc gacgtccagg
    20281 ccttgctcct cgaccttcgg ccgggacttc gagccctcct tcagcccgac ccgcaccggg
    20341 acgccagagt cgcgcaggct cagcgagtgt gcgtgcccct gactgccgta tccgatcaca
    20401 cccaccttgc ggccctggat gatcgacagg tctgcgtcgt cgtcgtagaa catcgacaac
    20461 ggctccccaa caacgtctgt cacgtgaata tctccttctt cttacgactt gacggtgccg
    20521 ataccgcggg gaccgcggga cagcgacacc attccggatt gggctatttc gcggatgccg
    20581 aaaggctcca gcacgcgcat tagcgcctcg atcttgccgc ggtccccggt ggcctcgatg
    20641 gtcagcgaat ccggcgatac gtcaatcacc ttggcgcgga agaggttcac cgcttcgatc
    20701 acctggctgc gggtgccggc gtccgcccgc actttgatca gcgccagctc tcgggacacc
    20761 gagttgccat catcctgctc gatgatcttg atgacgttga tcagcttgtt gagctgcttg
    20821 gtgatctgct cgagcggggt ttcctccgcg gagaccacga tggtcatccg cgacatgtcc
    20881 ttctgttcgg tggcacccac ggcaagcgac tcgatgttga agccgcgccg cgaaaacagc
    20941 gccgcgaccc gggccagcac accgggcttg tcttccacca gcaccgacag tgtgtgcgtt
    21001 ctcggtgcgt gtccgttgct catcaggcgt gcccttcgct ttcgtcgtcg aacagcgggc
    21061 ggatgccgcg ggcggcctgg atctcgtcat tgctggtgcc ggcggccacc atcggccaca
    21121 cctgtgcgtc ggcgccgacg atgaagtcga tcaccactgg acggtcgttg atcgcgcgtg
    21181 cctggttgat cacgtcgacg acgtcctctt cacgctcgca acgcaatccg acgcaaccca
    21241 gcgcctcggc cagcttcacg aaatccggga tgcgatgcga gtgcgtcgac aggtcggtct
    21301 gcgagtagcg ctcctggtag aagaggctct gccactgccg caccatgccc aggttgccgt
    21361 tgttgatcag cgccaccttg atgggcgcgc cttcgatggc acaggtggcc aactcctggt
    21421 tggtcatctg gaagcagccg tcgccgtcga ccgcccacac ctccgcgtcg ggatgggcga
    21481 tcttggcgcc catcgccgcc ggaatcgcga atcccatggt gcccagcccg ccggagttga
    21541 gccaggtgcg cggcttctcg taggagatga actgcgccgc ccacatctga tgctggccga
    21601 cgcccgccac atacaccgcg tccgggccgg cgatctcgcc cagcttctcg atcacgtact
    21661 cggggctcag gctgccgtcg ctctgcggcc cgtagctcaa cggataggtg gcccgaacgg
    21721 attccagata tgcccaccag tcggccatgt ccggggttgc ggaggcgcca gcgcccccgt
    21781 ggcgcagcat cgcgatcagc tcggtgagga cggctttgac atcgccgacg atcggcacgt
    21841 cggcgtgccg gttcttgccg atctccgccg ggtcgatgtc cgcgtggatg accttggcat
    21901 cgggcgcgaa cgagtcgagt tttccggtca cccggtcgtc gaaccgggtg cccagcgcga
    21961 tcagcaggtc gctgcgctgc agcgccccca ccgcggccac cgtgccgtgc atgccgggca
    22021 tgcccaggtt ctgcggatgg ctgtccggga acgcgccgcg cgccatcaag gtggtgacga
    22081 ccgggatgcc ggtgagttcc gccaattccc gcagttcctc ggtcgcctcg ccgcggatga
    22141 caccaccgcc gacgtagagc acgggtttgc gggcggcggc gatcagcttc gcggcctcgc
    22201 ggacctgccg gttgtgcggc ttggtgttcg gcttgtagcc gggcagatcc agctgcggcg
    22261 gccaactgaa cgtgcactgc gcttgcagca cgtccttggg gatatcgacg agcaccgcac
    22321 cgggccgtcc cgaggatgcg atgtagaagg cctcggccag cacccgggga atctcgtcgc
    22381 cggagcgcac caggaagttg tgtttggtga tcggcatggt gatgcccgag atgtcggcct
    22441 cctggaacgc gtcagtcccg atcagcccgc gcccgacctg cccggtgatc gcgactaccg
    22501 gtatcgagtc catctgcgcg tcggcgagcg gggtcaccaa gttggtggca ccgggacccg
    22561 acgtcgccat gcacaccccg accttgccgg tgacgtgtgc gtatccactg gcggcgtggc
    22621 cagcgccctg ctcgtggcga accagcacgt ggcgcagctt tttcgagtcg tacagcgggt
    22681 catagaccgg cagcacggcg ccgccgggaa tgccgaagat gacctcgacg tcgagttcct
    22741 ccaacgaccg gatcaccgac tgggcaccgg taagttgttc cggggcaacg tgtttcggtt
    22801 gcacggccgg agtttttgcg gcgggtttca ccgcgtcgga agcgacgtcg gccgcacgct
    22861 gcgtctgcgg cgtgtgtggc ctggtaggag cgctcactgt cctctaatcc ttattcacta
    22921 tgggagtctc ggggtgggca agaaaaaacc cccgtcagct cagctgctgc acgagggttg
    22981 gcgcgttggt gctggatcgc ctcaaattgc tccagggcta gtcaggcacc aacgcgccga
    23041 ctaattacta cgagcattcc gggctttccg gcggtatcca tagcgtccga cggtagcctt
    23101 cgcacagctc agccgtcaaa tccgctgcct ccgacggtgc gccgcgtccc gtccgcccgg
    23161 ggcggcgaca cgccgcgaac cagtcgtcgc gatacgcagc ctcggcgcgg gatagccgga
    23221 agtcgggcgg tgggatgatg cgagggtggt taccgattcc cccgtggtga tcagggtgtc
    23281 gccgatggcg catctcgccg tcggattcct ggccctgggc ttgctgatac cggtgctggc
    23341 ctggccgccg tccgcgccgc tgctgctcat accggtgctg ctgtcggcgt tgatcgtccg
    23401 gctgcgcacc gtcgccgacc acgacggcgt caccgttcgc accctgttgg ggagtcggac
    23461 cgtgccctgg gacgacatcg acggcctgcg cttccaccgg ggctcgtggg cacgagcccg
    23521 cctcaaaagc ggtggggagc tgcggttgcc cgcggtgacg ttcgcgacgc tgccgctgct
    23581 gaccgaagcg agcctggggc gagtccccaa tccctatcgc tgagtgtcag gcgatcggat
    23641 tgtcgccgtt gagcagcacc cacaccgcga tgccggcggc gatgcccagc agcagcgcaa
    23701 cgaacgaccc gatgaagggc cgcagggccc aaccccgccg ggcatccagg ccgtagcggc
    23761 ctggtccgca caggacgacc gcgacggcca tgacgatcaa ggtgatctgg tattcgtggc
    23821 cgtcgggcag gaagaacggg aacgagtgcg aatgcggccg ccccgagacc gaggccagca
    23881 ggccgttgat caggaacgcg agcgcgccgg ccgcggccac cggggtgaac aagccgatca
    23941 ccagcagcac accggccagg atctcgccgc cgacgctcac gtaggccagg atgtcggcgt
    24001 gctgatagcc gacgtcggac agtgagttct tgaatccggt cacgccctgg ccgccccacc
    24061 agccgaacag cttttgcagc ccgtgagcgc agagcaccac accgagccac acccgcagca
    24121 ccagcaaacc cagatgctgg gtgccgcgcc gaccggcgac gcggagcttg tcgttgtcgt
    24181 catcgccgtc gatctcggtg ggctgggccg ccgcgggcgc cacggactgc ggctggacgt
    24241 agggcagggg ttcctgctgg tcgagcaggc tgtatcccgc cgcagcgggg ccacccgagg
    24301 cagcggggtc gctgtacggg atgacggtgg tggttccaaa gtcgccgggg tacccgaccg
    24361 gtgtcaggtc atcttcgggg tccaccaagc gcgccgaggc ggggcgccca gggatcggct
    24421 ccggggattc cccggggcgc cgccaatgtg cgtcatgcga ttgactggtc acgggtgtca
    24481 gggtaagggc attcgggcct gaattgggga tttgagcggc gctttcggcg ggtaatcggc
    24541 ggttagcccg caaaatgggc ccggattgac cccaaatcgg ctcacccgcc cgtctttggg
    24601 gcccaagcag ggtccaaatt cgactaaaaa gggcccgcgc cccccgggcc agcgaacctt
    24661 tcccgaacct ttcggcgccg acacgagcac gcgtgtggcg gcggtgcccg cactgtccgt
    24721 agcctgtcgg tcatgcgact agggcgctcg gttcgcggcg ggctcgctgc gctctgtgcc
    24781 gcagtgctcg tatcgagtgg ctgcgcacgg ttcaacgacg cccagtccca acccttcaca
    24841 accaacccgg agcttcggcc ccaacccagt tcgacgcctc ccccgccgcc cccgctgccc
    24901 cccactccgt tccccaaggc atgcccggcg cccggggtga tgcaaggctg cctggaaagc
    24961 accagcggtc tgatcatggg caccgacagc aaaaccgccc tggtcgccga acgcaccacg
    25021 ggtgccgtca aggagatctc ggtcagcgcc gagccgaagg tgaagatggt gatcccggtc
    25081 gacccgtccg gcgacggcgg gctgatggac atcgtgctgt cgcccaccta caaccaagac
    25141 cgcctgatgt acgcctacat cagcacaccc accgacaaca gggtcatccg catcgccgac
    25201 ggcgacatcc ccaaggacat cctgaccggc atcccgaaag gcgccacccg gaacaccggg
    25261 gcgttgatct tcaccagccc caccaccctg ctcgtgatga ccggcgatgc cggcaacccg
    25321 gcgatggccg ccgacccgaa atcgttggcc ggcaaggtgc tgcgcatcga gcagccgacc
    25381 accatcggcc aggcgccgcc gacgacggca ctgtccgggg tcggctccgg cggcggcatg
    25441 tgcgtcgacc ccgtcgacgg ttcgctgtat gtcgcggacc gcaccccgac cgcggaccgc
    25501 ctgcagcgga tcaccaagac ctccgaggtc tccacggtgt ggacgtggcc ggacaaaccc
    25561 ggtgtggccg gctgcgcggc catggacggc accgtgctgg tcaacctgat caacaccaag
    25621 ctaacggtgg ccgtccggct cgcgccgtcg acgggcgcgg tcaccggtga acccgacgtc
    25681 gtccgtaagg acacccatgc ccacgcgtgg gcgctgcgga tgtcgcctga cggaaacgta
    25741 tggggcgcaa ccgttaacaa gaccgccggg gacgccgaaa a
//
LOCUS       DASSGQ010000173         2969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_711887,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000173 DASSGQ010000000
VERSION     DASSGQ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711887"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VFI55_07730"
     CDS             <1..611
                     /locus_tag="VFI55_07730"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HET7073953.1"
                     /translation="LAFVAALQHLSPLQRAVLVLRDVLGWQAGEVAEILETSTAAVNS
                     ALQRAHKQLANTRPMPEELSEPADADVRDVLDRYMAAFEHADVDMLAELLRADVELEM
                     PPIPTWFTGREAVREFLARVVLRAPDQWWLAYTRANGDPAFALYRRAPDGSFRAAGLD
                     ALSVIGGRIARIVAFNDPALVAKFGLPETITATPPRPGVAGT"
     gene            622..1200
                     /locus_tag="VFI55_07735"
     CDS             622..1200
                     /locus_tag="VFI55_07735"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HET7073954.1"
                     /translation="MPDPDELLLLAEATRYLLGSMLRVREPDLSAPTPCPDWDLRGLL
                     RHVRASLGDVTDVLAVPELRRTTGPGPNPAGADPVAALRAGIVDFLIASTLPPTAGRW
                     CEIDGRPLPAEVVVHVGEIEMVLHAWDIAQACRIRRPIPADLASALLWVSPPLAEAGL
                     GGHVFAEPLAAPTTATPGDRLLALFGRRPILR"
     gene            1295..>2969
                     /locus_tag="VFI55_07740"
     CDS             1295..>2969
                     /locus_tag="VFI55_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HET7073955.1"
                     /translation="MDCSSRLVAPGVPGLLRIEDCLDADGGVDLPSDATLISLINRNI
                     ANVGDAIAYRYLDYSRTADGRAAEVTWTQLGARMQAIGARVQHVAGRGERVAVLAPQG
                     IDYVAGFYAAIKAGTIAVPLFAPELPGHAERLDTALRDSEPTAVLTTTAAKDAVESFL
                     AGVPRLRRPHVIVIDQIPDSAGELFVPTELSVGDVSHLQYTSGSTRPPVGVEITHRAV
                     GTNLVQMILSIDLLDRNTHGVSWLPLYHDMGLSMIGFPAVYGGHSTLMSPAAFVRRPL
                     RWIHALSAGSRQGRVVTAAPNFAYEWTAQRGLPAPGDDVDLRNVVMIIGSEPVSIDAV
                     RTFNKAFAPYGLPRTAFKPSYGIAEATLFVATIEPSAEASVVYFDREQLSAGHAVRVP
                     EDAPSAVAQVSCGRTARSLWAVIVDPDTGTELPDGDVGEIWLQGENVGRGYWGLPDET
                     QLAFGAKLQTRLGEGSHTEGSTVEGTWLRTGDLGVYLDGDLYVTGRIADLVIIDGRNH
                     YPQDIEATVAEASPMVRRGYVTAFSVPADRLPGSGAGNADTGERLVIIAE"
BASE COUNT          436 a         1045 c         1005 g          483 t
ORIGIN      
        1 ggttggcgtt tgtcgctgcg ctgcagcacc tgtcgcccct gcaacgtgcg gttctggtgt
       61 tgcgcgacgt gttgggctgg caggccggcg aagtcgccga gatattggaa acgtccacgg
      121 cggccgtcaa cagcgccctg cagcgcgccc acaagcaact ggcaaacacc cgcccgatgc
      181 ccgaggagct cagcgaaccc gccgacgccg acgtgcgcga cgtgctggac cgctacatgg
      241 cggcgttcga acacgccgac gtcgacatgc tggcggagct gctgcgagcc gacgtcgagc
      301 tcgaaatgcc cccgattcca acgtggttca cgggacgcga ggcggtgcgc gaattcctgg
      361 cccgggttgt gctgcgcgcc ccggatcaat ggtggctggc ctacacccgg gccaatggcg
      421 acccggcgtt cgccttgtac cggcgggcgc cggacggttc cttccgtgcc gccggcctgg
      481 acgcgctgtc tgtgatcggc ggtcgcatcg cgcgcatcgt tgccttcaac gacccggcgc
      541 tggtcgcgaa gttcggcctg cccgagacga tcaccgcaac gccacctcgt ccaggcgtcg
      601 ccgggacatg aggctgcgac cttgcctgat cccgacgaac tgttgctgct ggccgaggcg
      661 acccggtatc tgctcgggag catgctccgg gttcgcgagc ccgatctctc ggcacccaca
      721 ccctgcccgg actgggacct gcgcggcctg ctgcgccacg tccgggcctc gttgggggac
      781 gtcaccgacg tgctggcggt cccagaactg cgccgaacta ccggtcccgg ccccaacccg
      841 gccggcgccg atccggtagc cgcccttcgg gccgggatcg tcgacttcct catcgcgtcg
      901 acgctgccac cgaccgcggg tcgctggtgt gagatcgatg gtcgccccct tcccgccgag
      961 gtcgtcgtcc acgtcggcga gatcgagatg gtgctgcacg cctgggatat cgcccaggcg
     1021 tgccgaattc gtcgacccat tcccgcggac cttgcttcgg cgctgttgtg ggtttcccca
     1081 ccgctcgccg aagccgggct gggcgggcat gtgttcgccg aaccgctggc cgccccgaca
     1141 acggccacac ccggtgaccg gctcctcgcg ctgttcggcc gccggccgat cttgcggtga
     1201 gcctggagga ggatcgcgtg ggcgccgcaa gggtgcgcgc tggaacgccg cacggggccc
     1261 gaagcggtcg gcgcgggcac ctagactgcg ctctatggat tgcagttccc ggctcgtcgc
     1321 ccctggtgtg cccggcctgc tacggatcga ggactgcctg gatgccgacg gcggcgtcga
     1381 tttgccgtcg gacgccaccc tgatatccct gatcaaccgc aacatcgcca acgtcggtga
     1441 cgcgatcgcg taccgctacc tggactacag ccgcacggcc gacgggcggg ccgccgaagt
     1501 gacctggacc caattgggtg cccgaatgca ggccatcggc gcgcgcgtgc aacacgtcgc
     1561 aggtcggggg gagcgggtgg cggtcctcgc gccgcaaggc atcgactacg ttgcgggttt
     1621 ctacgcggcg atcaaggccg ggaccatcgc cgtcccactc ttcgcgcccg agctgccggg
     1681 ccatgccgaa cgtctcgaca cggcgcttcg cgattcggag cccacggcgg tcctgaccac
     1741 aaccgcggcc aaggacgcgg tcgagagctt tctggccggc gtcccgcggc tgcgccggcc
     1801 gcacgtcatc gtcatcgatc agatacccga ctcggcgggg gagctgttcg tccccaccga
     1861 gctcagcgtg ggcgacgtct cgcacctgca gtacacctcg gggtcgacgc ggccccccgt
     1921 cggcgtggag atcacccacc gggcggtcgg caccaatctg gtgcagatga tcctgtcgat
     1981 cgacctgctg gaccgcaaca cccacggcgt cagctggtta ccgctctacc acgacatggg
     2041 cctgtcgatg atcggctttc ccgcggtgta cggcggacac tccacgctga tgtcgcccgc
     2101 cgcgtttgtc cgcaggccgc tgcgctggat tcatgctttg tccgccgggt cgcggcaggg
     2161 ccgcgtggtc accgccgcgc cgaacttcgc ctacgagtgg accgcacagc gcggcctgcc
     2221 cgcgccgggg gacgacgtcg atctgcgcaa cgtggtgatg atcatcggtt ccgaaccggt
     2281 cagcatcgat gcggtcagga cattcaacaa ggcgttcgcg ccgtacggat tgccgcgcac
     2341 cgcgttcaaa ccctcgtacg gcatcgcgga ggcgacgctg ttcgtggcga ccatcgagcc
     2401 gtccgctgag gcatcggttg tctatttcga ccgcgaacag ttgagcgccg gtcacgcggt
     2461 gcgcgtcccc gaagacgccc cctccgcggt ggcgcaggtg tcctgcggcc ggacggctcg
     2521 cagcctgtgg gcggtcatcg tcgaccccga taccgggacc gaactgcccg atggtgatgt
     2581 cggcgaaatc tggttgcagg gcgaaaacgt tggccggggc tattgggggc tgcccgatga
     2641 aacgcagctg gcgttcggcg ccaagttgca gacgcggctc ggcgagggca gccacaccga
     2701 gggctcgacc gtcgaaggca cctggctgcg caccggcgac ctcggggtgt atctggacgg
     2761 cgatctctac gtaaccggcc ggatcgcgga tctggtgatc atcgacggcc gcaaccacta
     2821 cccgcaagac atcgaggcca ccgtcgcgga agcctcaccg atggtgcggc gcggatacgt
     2881 gaccgcgttc tccgtgccgg ccgaccgact gccgggctca ggcgctggca atgccgacac
     2941 cggcgaacgc ctggtgatca tcgccgaac
//
LOCUS       DASSGQ010000174         7309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1015384,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000174 DASSGQ010000000
VERSION     DASSGQ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7309
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015384"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VFI55_07745"
     CDS             complement(<1..605)
                     /locus_tag="VFI55_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET7073956.1"
                     /translation="MFTIEELATIPLFSTLREKELEYLAGAVEDIHLIPGEYVGHEGE
                     GRSLAIVVEGKAELTKLVNGVEQVIGVRLPGEVGGEIPMTLGTPLPASMRAVEPSRVL
                     KVTVEVFHTLAAMAPQVSETVGAAALERMEMLRSATAQPREPAIFVIGPRVDPGVHRC
                     DSFLHRNQIPYERLDPDDPAAIPRIGGEAAAYPVVVLRDGTR"
     gene            complement(769..2178)
                     /locus_tag="VFI55_07750"
     CDS             complement(769..2178)
                     /locus_tag="VFI55_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HET7073957.1"
                     /translation="MPATDPTFYRSPAAAVSAAPEQIAYVAAFDPAGQAKDALAVVDC
                     DPNSSMYGGVVGWAELPTAGNELHHFGWNACSSALCHEGHGHHEAIERRYLLVPGIRS
                     STTYVLDTKPDPRRPVLAHTIQGGELAAKAGYSRPHTVHCGSGGIFMSALGGPNGDDG
                     PGGIALIDHDTFEVIGPWEVDRGPQFMSYDVWWHLNYDTVITSEWGTPSMVENGVNPE
                     DLLGRKFGHHVNFWSMSDRKLTQRVDLGDQHQMVLELRPAHDPAKAWGFVGVVTNAED
                     LSASVWLWHKDGDQWALDKVITIPAEPADADALPPLLKPFAAVPPFVTDIDLSVDDRW
                     LYVSCWGTGELKQYDVSDPFQPRETASVRLGGIVGRTTHPAAPDLPLGGGSQMIEISR
                     DGRRVYVTNSLYAAWDEVFYPDGVGAWMAKLDADISAGGLVPDVRFFPHGDDFRGLRV
                     HQTRLQGGDASSDSYCYTS"
     gene            2760..3206
                     /locus_tag="VFI55_07755"
     CDS             2760..3206
                     /locus_tag="VFI55_07755"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET7073958.1"
                     /translation="MATSSAAPASGDAPADRTIGATIDHNPLSDYEGYCTWGAQEQIH
                     VHTGYYIKALTGNAENWVDQAKAAGWMVVGVAQPRSIAVYGSWLIGGVGHVAWVDAVN
                     GNDITITEMNFGHGATAANGYRTTGFHEFDTRIATAVPGTSYILIP"
     gene            3349..5073
                     /locus_tag="VFI55_07760"
     CDS             3349..5073
                     /locus_tag="VFI55_07760"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HET7073959.1"
                     /translation="MILSIDLGKTRCRAAGGGRRAEGAGAPGLAAPGGVRAAEAAILA
                     LVSQLGNQFGPVEELIVGAAGALTAPGAARALGEALLASLQAERIAVTSDGVIAHAGA
                     LDGKPGVVLIAGTGVVALAIDADGGLRTADGWGPWLGDEGGGAWIGTAGLRAALRALD
                     GRGPSTSLIDAARARFGPPRTWPAQLTDAAALASFAPDVLAAQDDAVALAIVGAAAES
                     LAATARAAGSGPVAMVGGLAQVKALREQIDLVPPVGDALDGTLRLSAIHEPHVIRLSA
                     AVEAPAPNRLDALATEAVRPDLHDLDARPIGELVALMVAAEGNAHGAVVAALPRITAA
                     AEAIAARLERGGRLIYAGAGTPGRLGVLDAAECGPTFSTDIVRGVIAGGPAALTKAID
                     GAEDVFDPADLADLTSADALVGITASGRTPYVLGALDHARAAGALTVAIVNNSGSEAP
                     ADVVIELLTGPEVLAGSTRLTAGTSQKVVLNALSTSVMIALGKAYGPLMVDVRATNAK
                     LRRRTVRIVRDAAGVDEEAAIAALAAAGGHAKTAIVALLAGVDAAAAAVRLEHARGRV
                     RDALIGRS"
     gene            5073..6611
                     /locus_tag="VFI55_07765"
     CDS             5073..6611
                     /locus_tag="VFI55_07765"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7073960.1"
                     /translation="MRKAMRAATRKVAAVGIASLALVAACSRGDFAHTAVSETTPATT
                     PAQTSDRATVSKQPAAATAPGFATVSKLINDAIAADKLPGAVLVVGHGGDVAFHQAYG
                     SRKLASEPGLDGLPGPAEPMTEDTIFDLASLTKSLATATAVMQLYEQGKVRFDDPVQQ
                     HLPDFNTTNDPRRAQVTVRMLLTHTSGEPGDVELNDPWGLGRADKAEGVHRALVTPLE
                     SRPGEAFRYSDINFILLGALIERVTGEAEDAYVSEHVFAPLGMDETRYLPPANACGPH
                     TLRGAAIAWAPAPTGRAPAACPPGTWSTDLLSRIAPTARDEESRADPSKNPDIDHLLW
                     GTVQDTTARRMGGVAGHAGVFSTAHDVSVYAQALLDRLAGRPSQFPLTQTTLELMTTP
                     QQPGHTAGQLEAANAATREAIAKRPNTRDPLLAPRYPAIAGQNLRGFGWDIDTGQSTT
                     RGMVFPIGSFGHTGFTGTSIWLDPGSDTLIVLLSNSIHTRGSPPMSNLRGEVATATAR
                     ALGL"
     gene            complement(6623..>7309)
                     /locus_tag="VFI55_07770"
     CDS             complement(6623..>7309)
                     /locus_tag="VFI55_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET7073961.1"
                     /translation="MLAAGVVLVQCTSALPPPAATPSSAVIPSPPAASSSASEPPAQP
                     AGGTVEPVTAAELGASWRPGCPVEPGRLRRVNVDHIGFDGQTHRGELIVHEDLVPDVI
                     AIFEQLYRLGYPIDKIRTVDHYSDADDELSMEDNNTSAFNCRLIPGTDEWSPHAYGRA
                     IDINTLLNPCLYASGYFEPRNAAAYLDRSRTDPGLLHSGDPAERAFTDRGWQWGGYWR
                     TPDYQHFERP"
BASE COUNT         1266 a         2356 c         2470 g         1217 t
ORIGIN      
        1 cgagtaccgt cgcgcagcac caccaccgga taggcggcgg cctcacctcc aatgcgtgga
       61 atagctgcag gatcgtccgg gtctaggcgt tcataaggaa tctggttgcg atggaggaag
      121 ctgtcgcatc ggtgcactcc ggggtcgacg cgcgggccga ttacgaagat tgcgggctcg
      181 cgtggctgcg cggtggcgct tctgagcatc tccatccgct ccaacgcggc ggcaccgacc
      241 gtttcggaga cctggggggc catggcggcg agcgtgtgga acacctcgac cgtcaccttt
      301 agcacgcgcg atggttcgac ggctcgcatg ctggcgggca acggcgtgcc gagcgtcatc
      361 gggatctcgc ctccgacttc gcccggaagc cggacgccga tcacctgctc gactccgtta
      421 accagcttcg tcagctccgc cttaccctcg accacgatcg ccagggaccg gccttcgccc
      481 tcgtggccta cgtattcccc ggggatgaga tggatgtcct ccacggcccc agcaaggtac
      541 tccagttctt tctccctcag ggtcgagaag agcggaatcg tggcaagctc ctcgatggtg
      601 aacatgactg cctcccaacc tctttcacgg ggaagccgct gattcacgcg agcggctcag
      661 cgtgcggtgt cgaaattttc gatgcaggaa tcgtccatcg cacacctatg gctgcacatg
      721 aggcatccgc cccaaaaatg ttggggatcg acctcggctg gcgggcgttc agctggtgta
      781 gcagtaagag tcgctggacg cgtcaccgcc ttgcagccgg gtttgatgca ctcgcaggcc
      841 acggaaatcg tcgccatgcg ggaagaatcg gacatcaggg acaagcccgc ccgcggagat
      901 gtcggcgtca agtttggcca tccatgcgcc gacgccgtcg gggtagaaca cctcgtccca
      961 cgccgcgtac agcgagttcg tgacatacac ccggcggccg tcccggctga tctcgatcat
     1021 ctgcgagcca ccgccgagcg gtaggtccgg agccgcggga tgcgttgtgc gcccgacgat
     1081 ccctccgagc cgaaccgatg ccgtctcgcg cggttggaat gggtcgctga catcgtattg
     1141 tttgagttcg cctgtgcccc aacaggagac gtaaagccaa cgatcgtcga cggacaggtc
     1201 gatgtcggtg acgaacggtg gcacggctgc aaagggctta agcaacggtg gcaaggcgtc
     1261 ggcgtcggcc ggctcggcgg ggatagtgat caccttgtcg agggcccact ggtcaccgtc
     1321 cttgtgccac agccacactg acgccgatag gtcttcggcg ttggtcacta caccaacgaa
     1381 gccccacgct ttcgccggat cgtgtgcggg gcgtaactcg agcaccatct ggtgctggtc
     1441 gccgaggtcg acgcgctggg tcagctttcg atcggacatg ctccagaagt tcacgtggtg
     1501 tccgaacttc cttccgagca ggtcctcggg attaaccccg ttctcgacca tcgagggcgt
     1561 cccccactcg gatgtgatca cggtgtcgta gttcaggtgc caccacacgt cgtaggacat
     1621 gaattgcggt ccgcggtcga cctcccacgg tccgatgacc tcgaacgtat cgtggtcgat
     1681 cagcgcgatg ccgccgggac cgtcgtcgcc gttgggaccg cccaacgccg acatgaagat
     1741 gccgccggat ccacagtgca ccgtgtgcgg gcgggaatat ccggcctttg ctgcaagctc
     1801 gccgccctgg atcgtgtgcg ccagcaccgg ccggcgaggg tcgggtttag tgtcgaggac
     1861 ataggtggtc gacgaacgga tgccggggac tagcagatat cgccgctcga tcgcctcgtg
     1921 atggccatgt ccttcgtggc atagcgcgct cgagcaggca ttccagccga agtggtgcag
     1981 ttcgtttccg gccgttggca gctcggccca gccgacgacg ccgccgtaca tcgaggaatt
     2041 cgggtcacag tccaccacgg cgagcgcatc ctttgcctgg cccgccggat cgaaagcggc
     2101 aacgtaggcg atctgctcgg gcgcggccga aacggcggcg gctgggctgc ggtaaaacgt
     2161 gggatctgtt gcaggcatgg ttcctgcctc ctcgcgacaa ttcgccggac cgccgtttgg
     2221 gtacttggtc agttgacgtg gcagtgacgg acgctgtttc gatacttccc cgcgaatgcg
     2281 gtcctgtcca ttcccgcggc gatgtcgtca acttggatgg ggccgggcga agttgggggg
     2341 cgcaaggccg gccagagatt ctgcttcgcg caccgcgctg ctactgccgt cgcgttactg
     2401 gacggcagca gcggcggggt agtgcggcag ggtcggcctg accaacataa gcgccggaac
     2461 tatggcaatc gaggcgatcg tggcgtcgat catcgccggc catatcagcc ggttacccag
     2521 tgcgccggtt cacctcacga attcccccga agcctcgaag gtgaaggtcg cctctgggcg
     2581 ccgtgaaacg gatgctggcc gcccggcgat aacagaagcg taacgggttg ataccgcctt
     2641 ggcataggaa cgttcaggca gggaaacggg tgaagaccaa gggaaccagg ggatccaggg
     2701 gatccaaagg aattgtggcg gccgtcgccg tgagtggcgc actctcgctg ggagtttcaa
     2761 tggctacgtc gtctgcggca ccggcgagcg gggatgcgcc agcggaccga accataggcg
     2821 ccacgattga ccacaacccc ctcagcgatt acgagggcta ctgcacctgg ggagcccaag
     2881 aacagataca cgtccacacc ggctactaca tcaaggcgct caccggaaac gccgaaaact
     2941 gggttgacca agccaaggcg gcgggttgga tggtggtcgg ggtagcccag ccccgctcga
     3001 tcgccgtcta cggcagctgg ctcatcgggg gcgtcggaca cgtcgcctgg gtcgacgccg
     3061 tcaacggcaa cgacataacg atcaccgaaa tgaacttcgg gcatggggcc accgccgcca
     3121 acggctaccg caccacaggc ttccatgaat tcgacacccg catcgccaca gctgtcccgg
     3181 ggacgagtta catcttgatc ccctgagggg cttgatccct gaggcggcgc actcgctgtc
     3241 gtgtgtcaga agggcccata tctaagcggc gatgtcgcga ttgggaaaag ggcagcagca
     3301 ctgcaaaggc acgctccaga ccggcggcct cgtgaaaaca tggatccggt gatcctctcc
     3361 attgaccttg gaaagacgcg gtgtcgcgcc gccggaggcg gccgcagggc cgagggggcg
     3421 ggcgcgccgg ggctcgccgc gcctggggga gtgcgggccg cggaggcggc gatcctggcc
     3481 ttggtcagcc agctagggaa tcagttcggg ccggtcgagg agttgatagt gggcgccgcc
     3541 ggggcgctga cggcgccggg ggcggcgcgc gcgctgggcg aggcgttgct ggcctcgttg
     3601 caggcggagc gaatcgcggt gaccagtgac ggtgtgatcg ctcacgcggg cgcactggac
     3661 ggaaagccgg gggtcgtgct gatcgcgggc accggcgtgg tggcgctcgc gatcgacgcc
     3721 gacggcgggc tgcgtactgc cgacggctgg ggtccatggc tgggcgacga gggcggcggc
     3781 gcctggatcg gcacagctgg gctgcgcgcc gcgctccgcg ctctggacgg ccgcggaccg
     3841 tccacttcat taatagatgc cgcccgcgcc cgcttcggcc ccccgagaac ctggccggcc
     3901 cagctcaccg atgccgccgc gctcgcgtcg ttcgcgcccg acgtcctcgc tgctcaggac
     3961 gacgccgtgg cgttggcgat tgtcggcgcc gccgccgagt cactcgcggc gaccgccagg
     4021 gcagctggat cgggcccggt ggcgatggtt ggcggcctgg cacaggtgaa ggcgttgcgc
     4081 gagcagatcg acctcgtccc gcccgtcggc gacgcgctcg atggcacgct gcgcctcagc
     4141 gcaatccacg agccgcacgt gatccgcttg tctgcggccg tcgaagcccc tgccccgaac
     4201 agactcgatg cgctcgccac cgaagctgta cggccagacc tacacgacct cgatgcgcgg
     4261 ccgatcggcg agctcgtcgc gctaatggtc gccgccgagg gtaatgcgca cggagcggtg
     4321 gtagcggcac ttccgcggat cacggctgca gccgaagcga tcgccgcgcg gctcgagcgc
     4381 ggcggccgcc tgatctacgc cggcgccggg acgcccggtc ggctcggcgt gctcgacgcg
     4441 gccgaatgcg ggccgacatt cagcaccgac atcgtgcgtg gcgtgatcgc cggcgggccg
     4501 gcggcgctga cgaaggcaat cgacggcgcg gaggatgtgt tcgaccccgc agatctcgcc
     4561 gacctgacct cagccgacgc gctggtcggg atcacggcgt cggggcgcac gccgtatgtg
     4621 cttggggcgc ttgaccatgc acgcgcggcg ggcgcgctga ccgtggcgat cgtcaacaac
     4681 tcgggcagcg aggcgccagc ggacgtagtg atcgagctgc tgaccggacc agaggttctc
     4741 gccggctcca cgcggctgac cgctgggacg tcccagaagg ttgtgctcaa cgcgctctcc
     4801 acgagcgtga tgatcgcgct cggcaaggcg tacggaccgc tgatggtcga cgtgcgcgca
     4861 accaacgcga agctgcgccg ccgcacggtg cggatcgtcc gcgacgcggc cggcgttgac
     4921 gaggaggccg cgatcgccgc gctcgctgcc gcgggtggtc acgccaagac cgcgatcgtc
     4981 gcgctgcttg cgggcgtcga cgcggcggcg gctgcggttc ggctcgagca cgcgcgggga
     5041 cgcgtacgag acgccctcat cgggcggtcg tgatgcgaaa ggccatgcga gcggccacgc
     5101 gaaaggtcgc agccgttgga attgcctcgc tggcactcgt tgcggcttgc tcgcgcggcg
     5161 atttcgcgca cacagcagta agcgagacga ctccggcgac gacgcccgcc caaacatctg
     5221 atcgcgcaac ggtttccaag cagcccgctg cagctaccgc gcctggcttc gcaaccgtct
     5281 ccaaactgat caacgatgcg attgcggcag acaagctgcc gggtgccgtg ctggtagttg
     5341 gccacggtgg cgacgtcgcc ttccaccagg cttacggctc gcgcaagctt gccagcgaac
     5401 ccgggttaga tggattgccc ggacctgcgg agccgatgac cgaggacacg atctttgacc
     5461 tggcgtcgtt gacgaagagc ctcgcaacgg cgacagccgt catgcagctc tacgaacaag
     5521 gcaaggttcg gttcgacgat cccgttcaac aacatttgcc ggacttcaac accacgaacg
     5581 atccacggcg ggcacaggtg accgtccgca tgttgcttac gcacacttca ggcgaaccgg
     5641 gagacgtaga actcaacgat ccgtggggac ttggccgagc cgataaagca gaaggcgttc
     5701 atcgggcgct cgtgacaccg ctggagtcgc gtcccggcga agctttccgt tactccgaca
     5761 tcaacttcat tctgctcggc gcgctgatcg agagggtcac cggcgaggcc gaggacgcct
     5821 acgtttccga acacgtattc gcgccgcttg gcatggacga gacccgctac cttcccccgg
     5881 ccaacgcatg tggcccgcac acgcttcgag gcgctgccat cgcgtgggct cccgcgccaa
     5941 caggacgggc gccggccgct tgtccgccag gtacgtggag caccgatctc ctgtcgcgca
     6001 tcgcaccgac cgcacgcgac gaagagagcc gagccgaccc gagcaagaat cccgacattg
     6061 atcacttgct ttggggcacc gtgcaagaca caacggcacg gcgcatgggt ggagttgccg
     6121 ggcatgccgg cgttttctcg acggcacacg atgtcagcgt ctatgcgcag gccctgcttg
     6181 accggctcgc gggccgtccg agccagttcc cgctgacgca aacgactttg gagttgatga
     6241 cgactccgca gcagccggga cacacggctg gacaactcga agcggccaac gctgccaccc
     6301 gagaagccat cgcgaagaga ccgaatacca gggatccgtt gctcgcgccg cgctatccgg
     6361 cgatcgcggg gcaaaacctg cgcggcttcg gctgggacat cgataccggc cagtccacca
     6421 cgcgcggcat ggtcttcccc atcggtagct tcgggcacac gggcttcacc ggaacctcga
     6481 tttggctaga cccaggctca gataccttaa tagttctgct gtcgaactcg atccatacgc
     6541 ggggcagtcc accgatgtcg aacctgcgcg gtgaggttgc gacggcgacg gcccgcgccc
     6601 tgggtctcta aagcattgcg gctcagggcc gctcgaagtg ctggtagtcg ggagtcctcc
     6661 agtagccacc ccattgccag ccacgatcgg tgaaggcgcg ctcggccggg tcgccgctgt
     6721 gcaaaagacc ggggtctgtg cggctgcggt ccaggtaggc cgccgcgttc cgcggttcga
     6781 aatatccgct ggcgtagagg cacgggttga gaagcgtatt aatgtcgata gcccggccgt
     6841 aagcgtgtgg cgaccattcg tcggtccctg gaatgagtcg gcagttgaat gccgaagtgt
     6901 tgttgtcctc catggataat tcatcatcgg cgtccgagta gtggtcgacc gtgcggatct
     6961 tgtcgatggg atagcccagt cggtaaagct gctcgaagat cgcgatgaca tccggcacca
     7021 ggtcttcgtg cacgatcagc tcgccgcggt gggtctggcc gtcgaaaccg atgtggtcca
     7081 cgttgacccg ccgcagccgc cccggctcga cggggcaacc tggccgccag ctcgcgccga
     7141 gttcggcggc ggtgaccggc tcaacggtcc caccggcagg ttgtgctggc ggctcgctgg
     7201 ccgacgacga cgcagccggc ggtgacggta taacagccga tgatggtgtg gccgcaggag
     7261 gtggcagcgc ggaggtgcac tgcaccagca cgacacccgc tgcgagcat
//
LOCUS       DASSGQ010000175         3285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_316711,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000175 DASSGQ010000000
VERSION     DASSGQ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316711"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..668
                     /locus_tag="VFI55_07775"
     CDS             195..668
                     /locus_tag="VFI55_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7073962.1"
                     /translation="MTLLQGALADRDKWSAVGECAIEKTMAAVGTKSAMLIMREAYYG
                     TTRFDDFAERVGITKAATSARLAELVDLGLLARRPYREPGQRTREEYVLTDAGVDFMP
                     VVWAMFEWGRRHLPGRNRLRLTHQGCGAQAGVEIRCTEGHLVPPDELGMRLVKSP"
     gene            complement(655..1647)
                     /gene="rsgA"
                     /locus_tag="VFI55_07780"
     CDS             complement(655..1647)
                     /gene="rsgA"
                     /locus_tag="VFI55_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET7073963.1"
                     /translation="MKPGDYDESDVKVRSGRGSRPRTKTRPEHAEAEAAMVVTVDRGR
                     WGCVLGGRPDRRVTAMRARELGRTPVVVGDDVDLVGDLSGRTDTLARIVRRGPRRTVL
                     RRTADDTDPTERVVVANADQLLIVVALADPPPRTGLVDRALIAAYAGGLTPILCLTKT
                     DLAPPEPFAEQFVDLDLTVVAAGVDDPLLAVADLLTGKITVLLGHSGVGKSTLVNRLV
                     PDADRAVGEVTEIGRGRHTSTQSVAFPLQTTPEGTGWVIDTPGIRSFGLAHIEPDDVL
                     MAFSDLSDAIRDCPRGCGHMGPPADPECALDGLSGPAVRRVAAARRLLAALRET"
     gene            complement(1644..2948)
                     /gene="aroA"
                     /locus_tag="VFI55_07785"
     CDS             complement(1644..2948)
                     /gene="aroA"
                     /locus_tag="VFI55_07785"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET7073964.1"
                     /translation="MTAWAAPLATEPVHATVTVPGSKSQTNRALVLAGLAAAQGQGVS
                     TISGALRSRDTDLMIGALRALGLDVEGAGSELTVAGRIAPGPGARVDCGLAGTVLRFV
                     PPLAALADSVVEFDGDEQARARPIAPLLDALRVLGVPIDGSGLPFRVQGSGSVAGGTA
                     AIDASASSQFVSGLLLSAASFTDGLTVQHTGSEVPSAPHIAMTIVMLRQAGVDVDDSV
                     RNRWQVHAGPVAARHWDVEPDLTNAVPFLAAAVVSGGTVRITNWPANSVQPADNILSV
                     LSKLNATVTQTDSFLEVRGSADYGGFDVDLRAVGELTPSVAALAALATPGSVSRLSGI
                     AHLRGHETDRLAALCAEINRLGGDCTETPDGLVITATPLRPGIWRAYADHRMAMAGAI
                     IGLRVAGVQVDDIGATSKTLPEFPQLWARMLAGESSEGEPGI"
     gene            3069..>3285
                     /locus_tag="VFI55_07790"
     CDS             3069..>3285
                     /locus_tag="VFI55_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HET7073965.1"
                     /translation="MCGRFAVTTDPALLAQKIKAIDEATAPKADAPDYAESPNYNVAP
                     TTTIATVVTRHSEPDDQPTRRVRLMRWG"
BASE COUNT          530 a         1146 c         1128 g          481 t
ORIGIN      
        1 gcgggcagca cgtcgtgcaa ggcgccactg gcctttccct tgccgttcgg ggtgcgaacg
       61 gcgcctacga ggacggcgtc gcgaccttgg tagctggcca tggctcctcc ttgagtcctc
      121 agctgggtat cactcgctgt actcaggtaa acacctgagt atactgaaag caacccagat
      181 gggaggtgat ctgcgtgaca cttttgcagg gcgcgctcgc cgaccgggac aagtggtcgg
      241 cagtgggtga gtgtgcgatc gaaaagacca tggcggcggt cggcaccaaa tccgccatgc
      301 tgatcatgcg cgaggcgtac tacggcacca cccgattcga cgacttcgcc gagcgggtgg
      361 gaatcaccaa ggccgccacg tctgcccggc tggccgaact ggtcgacctc ggcttgctgg
      421 cccgacggcc ttaccgcgag cccgggcagc gcacccgcga agagtatgtg ctcaccgacg
      481 ccggcgtcga cttcatgccg gtggtctggg cgatgttcga gtgggggaga cgccacttgc
      541 cgggccgcaa tcgtctgcgg ctcacgcatc agggctgcgg ggcgcaggcg ggtgtcgaaa
      601 tccgctgcac ggaaggccat ctggtgcctc ccgacgagct gggtatgcgg ctggtcaagt
      661 ctccctgagc gcggccagta gccgccgggc cgcggccacg cggcgaacgg cgggaccgga
      721 cagcccgtcc agcgcgcatt cggggtcggc cgggggcccc atatgcccgc agccgcgggg
      781 gcagtcgcga attgcgtcgg acagatcgga aaaggccatc agtacgtcgt cgggctcgat
      841 gtgggccaac ccgaacgagc ggattcccgg ggtgtcgatc acccagccgg tgccttccgg
      901 tgtcgtctgc aacggaaacg ctaccgactg cgtcgacgtg tgccggcccc tgccgatctc
      961 ggtcacctct ccgacggccc gatccgcgtc gggcacaagc cgattcacca atgtcgactt
     1021 gccgaccccg gaatgtccga gcagcacggt gattttgccg gtcagcaaat ccgccacggc
     1081 gagcagcgga tcgtcgacgc ccgcggcaac cacggtcaag tcgaggtcga cgaactgctc
     1141 ggcgaacggc tccggcgggg cgaggtccgt cttggtcaga cacagaatcg gggtcagtcc
     1201 gccggcgtag gcggcgatca acgcccgatc caccaggccg gtgcgcggcg gcgggtcggc
     1261 cagcgccacc acgatcaaca gctggtcggc attggcgact accactcgtt cggtcggatc
     1321 ggtgtcatcg gcggtgcgcc gcaacaccgt tcgccgcggc ccccggcgca cgatccgggc
     1381 cagcgtgtcc gtgcgaccgg acagatcgcc gaccagatcg acatcgtcgc cgacgaccac
     1441 cggagtgcgg cccaactcgc gcgctcgcat ggccgtgact cgccgatcgg ggcgcccgcc
     1501 cagcacacaa ccccaccggc cgcggtcgac agtgaccacc atggcggcct cggcttcggc
     1561 gtgctcggga cgggtcttgg tccgcggtcg cgagcccctg ccggaccgga ccttgacgtc
     1621 tgactcgtcg tagtcgcctg gcttcaaatg ccgggctctc cctcgcttga ttcgccggcc
     1681 agcatgcggg cccacagttg cggaaactcc ggcagggtct tgctggtggc gccgatgtcg
     1741 tccacctgga ctccggcgac ccgcaacccg atgatcgcgc cggccatcgc catccgatgg
     1801 tcggcgtagg cccgccaaat gccgggccgc agcggtgttg cggtgatcac caggccgtcg
     1861 ggcgtttcgg tgcagtcgcc gccgaggcgg ttgatctcgg cgcacaacgc ggccagccga
     1921 tcggtctcat ggccccgcag atgggcgatg ccgctcagtc gtgacaccga gccgggggtg
     1981 gccagcgcgg ccagcgccgc gaccgacggt gtcagctcac cgacggcgcg caggtcgacg
     2041 tcgaagccac cgtagtccgc tgacccccgc acctcgagga acgaatcggt ctgagtgaca
     2101 gtggcattca gcttgctcaa gacgctcagg atgttgtcgg cgggttgcac gctgttggcg
     2161 ggccaattgg tgatgcgcac ggttccgccg ctgaccacgg ccgccgccag gaacggaacg
     2221 gcattcgtca gatccggttc gacgtcccaa tggcgggccg cgacggggcc ggcgtgcacc
     2281 tgccagcggt tgcgaaccga gtcgtcgacg tcgacccccg cctgccgcag catgacgatc
     2341 gtcatcgcga tgtgcggcgc cgacggcacc tccgaaccgg tgtgctggac ggtcaggccg
     2401 tcggtgaagg acgccgcgga cagcagcagg cccgatacga actgcgacga cgcggaggcg
     2461 tcgatggccg cggtgccgcc ggcgaccgac ccgctgccct ggacccgaaa aggcagaccg
     2521 ctgccgtcga tcgggacgcc gagaaccctg agcgcatcga gcagtggcgc gatgggtcgg
     2581 gcccgcgctt gctcgtcgcc gtcgaactcc accaccgaat cggccagcgc cgccagcggc
     2641 ggaacgaaac gcagaaccgt gccggccagc ccgcaatcga cccgcgcgcc gggcccgggc
     2701 gcaatacggc cggcgacggt cagttcggat cccgctccct cgacgtccaa accgagggcg
     2761 cgcagcgccc cgatcatcag gtcggtgtcc cggctgcgca gcgctccgct gatggtcgag
     2821 acgccttggc cttgcgccgc ggccagcccc gccagcacca acgcccggtt cgtctgcgac
     2881 ttcgagccag gaacggtcac ggtcgcgtgc accggttcgg tggccagagg ggccgcccag
     2941 gccgtcaggg gcgcggtact actcacgtgc ttcatcctgc cgtgtcagcc ggcgccgtgg
     3001 gtaccgggcc actaccggag ttcggtgcgg ggcactgcag gagtcctgcg cgaggcacta
     3061 tgggagtcat gtgcggacgg tttgcggtca cgacggatcc ggccctgctg gcccagaaaa
     3121 tcaaggcgat cgacgaggct accgccccga aagcggatgc gcccgactac gcggaatccc
     3181 ccaactacaa cgtggccccg acgacgacga tcgcgaccgt cgtcacccgg cacagcgagc
     3241 ccgacgacca accgacccgc cgggtgcgcc tgatgcgctg gggtc
//
LOCUS       DASSGQ010000176         4179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_286847,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000176 DASSGQ010000000
VERSION     DASSGQ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286847"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /locus_tag="VFI55_07795"
     CDS             <1..1383
                     /locus_tag="VFI55_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET7073966.1"
                     /translation="ADTHGNVVHLGERECSLQRRHQKVIEEAPSALLDPRTRARIGAA
                     ACNTARSVDYVGAGTVEFIVSAARPDEFFFMEMNTRLQVEHPVTEAVTGLDLVEWQLR
                     VAAGEKLAFAQDDIELRGHAIEARVYAEDPARGFLPTGGQVLQVFEPSGDGVRVDSSL
                     LPGTLVGSDYDPMLSKVIAYGTDREEALAKLDRALAQTAVLGVQTNVEFLRFLLADER
                     VRAGDLDTALLDERLADFAPLPAPDDVLAAGGLYRQWALSFRARWARRGGGNAWAVPT
                     GWRVGGGVAPVRTAMRTPLRSETLSVWGPPENARVQVGDGEIRSGSVQVEPTRMSATL
                     DGLRRDYRWAEADRHLWVADERGTWHLREAEERKIHRDAGARQAEILSPMPGTVIAVQ
                     VTSGARVSEGDVVVVVEAMKMEHSLAAPVSGRAEVLVSVGDQVSVDQVLARLVPEEPD
                     ASADSSKDQT"
     gene            1380..2546
                     /locus_tag="VFI55_07800"
     CDS             1380..2546
                     /locus_tag="VFI55_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7073967.1"
                     /translation="MTTSITAGTLPKEYEDLRDTVAEFARTVVAPVSAKHDEEHSFPY
                     EVVAKMGEMGLFGLPFPEEYGGMGGDYFALSLALEELGKVDQSVAITLEAGASLGAMP
                     IYRFGTEEQKQRWLPDLTAGRALAGFGLTEPGSGSDAGGTRTTARNDDGEWVINGTKQ
                     FITNSGTDITSLVTVTAVTGKLADGKKEISTIIVPSGTKGFTVEPVYSKVGWNASDTH
                     PLSFDDARVPQENLLGGRGTGYANFLSILDEGRIAIAALATGAAQGCVDESVKYAKER
                     QSFGRPIGTYQAISFKIARMEARAHVARAAYYEAAAKMVAGKPFKKEAAIAKMIASEA
                     AMDNARDATQIHGGYGFMNEYPVARHYRDSKILEIGEGTTEVQLMLIARSLGLP"
     gene            2543..3034
                     /locus_tag="VFI55_07805"
     CDS             2543..3034
                     /locus_tag="VFI55_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET7073968.1"
                     /translation="MTGKSIVQRGLWFEEFEIGTTYLHRPGRTVTEADNVLFTTLTMN
                     TQSLHLDAAWAADQPGFRGERLANSMFTLSTLVGLSVAQLTLGTIVANLGFSEVSFPK
                     PVFHGDTLYAETVCTGKRESKSRPGEGVVTLEHTGRNQHGDVVARAVRSTLVQKRPDD
                     GES"
     gene            3034..3846
                     /locus_tag="VFI55_07810"
     CDS             3034..3846
                     /locus_tag="VFI55_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HET7073969.1"
                     /translation="MNLRAVGPGWLFCPADRPERFAKAAAAADVVILDLEDGVAAADK
                     PAARTALRATQLDPERTVVRINATGTDEQARDLDALADTAYTTVMLPKAESAAQVAAL
                     APREVVALVETPRGALFAAEIAAAGGTVGMMWGAEDLVATLGGSSSRRPGGTYRDVTR
                     HVRSTILLAASAFGRIALDAVHLDIRDIEGLRAEADDAVAVGFGGTVCIHPTQIPVVR
                     EAYRPSREKLDWARRVLAAARTQRGVFAFEGRMVDSPVLKHAEMMLRRAGEP"
BASE COUNT          702 a         1329 c         1516 g          632 t
ORIGIN      
        1 gccgacacgc acggcaacgt ggtgcatctg ggcgagcgtg aatgcagcct gcagcggcgc
       61 caccagaagg tcatcgaaga ggcgccctca gcgctgcttg atccccggac acgagcgcgg
      121 atcggtgcgg cggcctgcaa caccgcccgc agcgtcgact acgtcggggc gggcaccgtg
      181 gaattcatcg tgtccgccgc ccggccggac gagttcttct tcatggagat gaatacccgc
      241 ctgcaggttg agcatccggt gaccgaagcg gtcaccggac tggacctggt ggagtggcag
      301 ctacgcgtgg ccgccggcga gaaactggcg ttcgcccagg acgacatcga attgcgcggg
      361 cacgcgatcg aggcccgcgt gtacgccgag gatccggccc gcggatttct gcccaccggg
      421 ggtcaagtgc tgcaggtttt cgagccgtcc ggcgacggcg tgcgggtgga ctcgtcgctg
      481 ctgcccggca cgctggtcgg cagcgactac gacccgatgc tgagcaaggt gatcgcgtac
      541 ggaaccgacc gcgaagaggc gctcgcgaaa ctcgatcggg ccctggcgca gaccgcggtc
      601 ctgggcgtgc agaccaacgt cgagttcctg cgcttcctgc tggccgacga gcgggtgcgt
      661 gcaggcgacc tcgacaccgc gctgctggac gagcggttgg cggacttcgc gccgctaccg
      721 gcgcccgacg acgtgctcgc cgccggcggt ctctaccggc agtgggccct ttctttcagg
      781 gcgcgctggg cccgccgcgg tgggggcaac gcgtgggcgg tgccgacggg atggcgggtc
      841 gggggtggcg tggcgccggt gcgcaccgcg atgcgcaccc cgctgcgcag cgagacgttg
      901 tcggtgtggg ggccgcccga aaacgccagg gtgcaggtcg gcgacggcga gatccgttcc
      961 ggcagtgtgc aagtggagcc aacccgaatg agcgcgacgc tggacgggct gcgccgcgac
     1021 taccggtggg ccgaggccga ccggcacctg tgggtcgccg acgagcgggg aacctggcac
     1081 ctccgcgagg ccgaggaacg caagatccac cgcgatgccg gggcgcggca ggccgagatc
     1141 ctcagcccga tgccgggcac cgtgatcgcc gtgcaggtca cctccggcgc ccgggtgtcc
     1201 gagggggacg tggtggtggt cgtggaggcg atgaagatgg aacattcgct ggccgcaccg
     1261 gtttcgggcc gagccgaggt gctggtgtcc gtcggcgatc aggtgagcgt cgaccaggtg
     1321 ctggctcggc tggtacccga agagcctgat gcatcggccg attcgagtaa ggaccagaca
     1381 tgacgacatc cattacggcg ggaacgttgc cgaaggaata cgaggatctt cgtgacacgg
     1441 ttgccgagtt cgcccgcacc gtcgtcgcgc cggtatcggc caaacacgat gaggaacaca
     1501 gcttcccgta cgaagtcgtc gccaagatgg gggagatggg gctcttcggc ctgccgttcc
     1561 ccgaggaata cggcggcatg ggcggcgact acttcgcgtt gtcgctggcg cttgaggaac
     1621 tcggcaaggt cgaccaatcg gtggcgatca cgctggaggc cggggccagc cttggcgcca
     1681 tgccgatcta ccgattcggc accgaagagc agaagcaaag gtggctgccg gacctcaccg
     1741 ccgggcgggc gctggccggc ttcggcctca ccgagccggg gtccggctcc gacgcaggcg
     1801 gcacccgcac caccgcccgg aacgacgacg gcgagtgggt gatcaacggc accaagcaat
     1861 tcatcaccaa ctcgggcacc gatatcacct cactggtcac ggtcaccgcg gtcaccggaa
     1921 agctcgccga cggtaagaag gagatctcga cgatcattgt gcccagcggc acaaagggat
     1981 tcaccgtcga accggtctac agcaaggtcg gctggaacgc gtcggacacg cacccgttga
     2041 gcttcgacga cgcccgcgtc ccgcaagaga atctgttggg tggccgcggc accggctacg
     2101 cgaacttcct gtccatcctg gacgagggcc gcatcgcgat cgccgcgctg gcgaccggag
     2161 cggcgcaggg ctgcgtcgac gagagcgtca agtacgccaa agaacgccag tcgttcggcc
     2221 ggccgatcgg cacctatcag gcgatcagct tcaagatcgc gcgaatggaa gcgcgtgccc
     2281 acgtcgcccg cgcggcgtac tacgaagcgg ccgcaaagat ggtggcgggc aagccattca
     2341 agaaggaggc ggcgatcgcg aagatgatcg cgtcggaggc ggcgatggac aacgcccgcg
     2401 acgccacaca aatccacggc ggctacggct tcatgaacga atatccggtg gcgcgtcatt
     2461 atcgcgacag caagatcctc gagatcggtg agggcaccac cgaggtgcag ctgatgctca
     2521 tcgcgcgatc gctgggcctg ccatgaccgg gaagtcgatt gtccagcggg gcttgtggtt
     2581 tgaggagttc gagatcggga cgacatacct gcaccggccg ggccgaacgg tcaccgaagc
     2641 cgacaacgtg ttgttcacca cgctgacgat gaacacccag tcgctgcacc tggacgccgc
     2701 gtgggctgcg gaccaaccgg gctttcgggg tgagcggctg gcgaactcga tgttcaccct
     2761 ttccacgctg gtgggcctgt cggttgcgca gctgacgctg gggaccatcg tggccaacct
     2821 cgggttttcc gaggtgtcgt tccccaagcc ggtctttcac ggcgacaccc tgtacgccga
     2881 gaccgtctgc accggcaagc gcgagtcgaa aagccggccc ggcgaaggcg tcgtcactct
     2941 cgagcacacc gggcgcaacc agcatggcga cgtcgtcgcg cgggcggtgc gcagcactct
     3001 ggtgcagaag cggccggatg acggagagtc ctgatgaacc tgcgcgccgt gggtcccggg
     3061 tggctgttct gcccggccga ccgccccgag cgctttgcga aggctgctgc cgccgccgat
     3121 gtggtgatcc tcgacctcga ggacggggtg gccgcggcgg ataagcccgc tgcccgcacg
     3181 gcactgcggg cgacgcagct ggaccccgag cgcaccgtgg tacggatcaa cgcgaccggt
     3241 actgacgagc aggcccgcga cctggacgcg ctggcggata cggcttacac gacggtgatg
     3301 ctcccgaagg ccgaatcggc cgcgcaggtg gccgcactcg cgccgcggga ggtcgtcgca
     3361 ctcgtggaga ctccacgcgg tgcgttgttc gccgcagaga tcgccgcggc ggggggcacg
     3421 gtggggatga tgtggggtgc cgaggatctg gttgccaccc tgggaggcag ctcgagccgg
     3481 cggcccggag gcacctatcg ggacgtcacc cgccacgtgc gttcgacgat cctgctcgcg
     3541 gcgtccgcat ttggccggat cgcgctggac gccgtgcatt tggatatccg cgacatcgag
     3601 ggcttgcggg cggaggccga tgacgccgtg gcggtgggtt ttggcgggac cgtctgcatc
     3661 cacccgactc agatcccggt ggtacgcgag gcctaccggc ccagccggga gaagctggat
     3721 tgggcgcgaa gggtattggc ggcggcgcga acccagcgcg gggtgttcgc gttcgaggga
     3781 cggatggtcg actcgccggt gctcaaacac gcggaaatga tgctgcggcg ggccggggag
     3841 ccctaggcgg cgtgccggcg ccgttcgccc ggccaagttg ctgggcacac gccggtcgcg
     3901 tcgggtgacc ctaacaccgg agcgacttga cccgaccgga tccggtcgcc gcgacacgtt
     3961 tcggacccga ccgcgatcag cgtgctgtct ttgcatttga gcgcataatg ggcagtacgc
     4021 cagtgtcgcg gcgagcgaag cgtttcgtgc agccggcgat cgacttcggc tcgcggcagg
     4081 ttccggctaa ggatttgctg tggccgacaa gccatgcggc cctgggctaa ggaggcggta
     4141 tggcggatcg ttcgcagatg ccgatgactg tcgacctcg
//
LOCUS       DASSGQ010000177        10381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_468920,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000177 DASSGQ010000000
VERSION     DASSGQ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10381
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468920"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..2007)
                     /locus_tag="VFI55_07815"
     CDS             complement(88..2007)
                     /locus_tag="VFI55_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HET7073970.1"
                     /translation="MGPTPFKPSINWSTAFTDSLRWIAIAWVIGAIALLAVLVAFRYL
                     TPWGRQFWRITRGYFVGRASVKVWAVLGVLLFSVLLSVRLTVLLSYQGNDLYTAVQKA
                     VQGVAAGNARVKESGIHGFWMSLVIFSLLAILYVIRFMADIYLTQRFIIAWRVWLTAH
                     LTDDWLDGRAYYRDRFIDNTIDNPDQRIQQDVDIFTANAGGTPNIPSNGTGSTLLFGA
                     VNAVASVISFAAILWDLSGDLNLFGVNFPRAMFWTVLVYVLIATVVAVWLGRPLIWLS
                     FNNEKLNAAFRYALVRLRDAAEAVGFYRGERVERAQLWRRFTPIIGNYRKFVRRTIIF
                     NGWNWSVSQAIVPLPWIIQAPRLFAGQIDFGDVGQTATAFGNIHDSLSFFRNNYDAFA
                     AFRAAIIRLHGLVDADARGRALPSLLVKPSEEVAVELAHVEVRTPAGDQLIDSLDVQL
                     DHGESLVITGRSGAGKTTLLRSLAELWPYASGTLCRPDGENATMFLSQLPYVPLGSLR
                     IVVCYPNSPDDIAEGTLRDTLTKVALAPLCDRLDEERDWAKVLSPGEQQRVAFARILL
                     TKPKAVFLDEATSALDEGLEFALYELLRSELPDCVVVSVSHRRAVEQHHTRQLRLLGD
                     GQWRLGPVEKEPVRV"
     gene            2337..4673
                     /gene="secA2"
                     /locus_tag="VFI55_07820"
     CDS             2337..4673
                     /gene="secA2"
                     /locus_tag="VFI55_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HET7073971.1"
                     /translation="MSKSTRTQPGRLSSRFWRLLGATTEKNQSRSLTEVTKSSEYDDE
                     AAGLSDEQLRKAAGLLKLEDLADSDDMAQFLAIAREAAERATTLRPFDVQMLGALRML
                     AGDVIEMATGEGKTLAGAIAAAGYALAGRHVHVVTINDYLARRDAEWMGPLVEAMGLT
                     VGWITAESTSEDRRAAYDCDVTYASVNEIGFDVLRDQLVTDVADLVSPNPDVALIDEA
                     DSVLVDEALVPLVLAGTTHRETPRLEIITLVGELVGDKDADEYFATDSDSRNVHLTDL
                     GARKLEKALGGIDLYSEEHVGTTLTEVNVALHAHVLLQRDVHYIVRDDAVHLINASRG
                     RIAQLQRWPDGLQAAVEAKEGIETTETGEVLDTITVQALINRYATVCGMTGTALAAGE
                     QLRQFYKLGVSPIPPNTPNIREDESDRVYITAAAKNDAIVAHIAEVHETGQPVLVGTR
                     DVAESEELHERLLRRGIPAVVLNAKNDAEEAKVIAEAGEFGTVTVSTQMAGRGTDIRL
                     GGSEEADHDRVAELGGLHVVGTGRHHTERLDNQLRGRAGRQGDPGSSVFFSSWEDDVV
                     AANLDQNKLPMQTDEDGKIVSPKAAGLLDHAQRVAEGRMLDVHANTWRYNQLIAQQRA
                     IIVDRRNTLLRTATAREELAELTPKRYKELSKQLSEERLETICRLIMLYHLDRGWADH
                     LAYLADIRESIHLRALGRQNPLDEFHRLAVDAFASLAADSIEAAQQTFETANVLDEEP
                     GLDLSKLARPTSTWTYMVNDNPLSDDTLSTLSLPGVFR"
     gene            4775..5380
                     /locus_tag="VFI55_07825"
     CDS             4775..5380
                     /locus_tag="VFI55_07825"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET7073972.1"
                     /translation="MEQVPPPDRVLTVPNVLSAIRLVLIPVFVYALLFAHANGWAVAI
                     LMFSGASDWADGKIARTLNQSSRLGVLLDPAVDRLYMVTVPVAMAVDGIVPWWFVIML
                     LARDGLLAATLPVLRSRGLSALPVTYFGKAATFALMAGFPLVLLGTWDASWSRILRAV
                     GWGFLGWGMYAYLWSFVLYVVQMVLVVRRMPKVQPVPGEHG"
     gene            5373..6296
                     /locus_tag="VFI55_07830"
     CDS             5373..6296
                     /locus_tag="VFI55_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HET7073973.1"
                     /translation="MADADRLLGGYDPNAGRSAHTAARPTLIPVPSLLRALLSEHLDP
                     GYAAAAAKRSGPMAARTGRERAVGWFWQALAALLIATVFAAAVAQARSVAPGVHTAQE
                     LLARSVRSTEASAAKLGQQRSALSTRVDDVQRHALADDAEGQRLLNRLDALSLAAAST
                     PVTGPGLTVTVTDPGVSPNLSDVSKQRVSGSRQIILDRDLQLVVNSLWASGAEAISVG
                     GVRIGPNVTIRQAGGAILVDNNPTGSPYTILAVGPPHAMRDTFDHSPGLQRLRLLEAS
                     YGVVVSVNVGDSLSLPAGSVRDVKFAKQIGP"
     gene            6355..6687
                     /locus_tag="VFI55_07835"
     CDS             6355..6687
                     /locus_tag="VFI55_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HET7073974.1"
                     /translation="MIGIAALAVGIVLGLVFHPAVPEVIQPYLPIAVVAALDAVFGGL
                     RAYLERIFDPKVFVISFVFNVFVAALIVYVGDQLGVGTQLSTAIIVVLGIRIFGNAAA
                     LRRRLFGA"
     gene            6816..7646
                     /locus_tag="VFI55_07840"
     CDS             6816..7646
                     /locus_tag="VFI55_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HET7073975.1"
                     /translation="MRHGHPDADSTADDGAQHGRHELPAKRPRPEIGPVHRTGLSALL
                     QGGRARLVFGTLAILLCLVLGIAIATQVRQTKSGDSLDTERPADLLVLLDSLRQREAT
                     LNTEVSELQNTLNSLQASGNTDQAAIHSAQARLAALSILVGSVGATGPGVTLTIDDPG
                     PGVSPEAMLDVINELRAAGAEAIEINDAHQSVRVGVDTWIVGTPGTLTIDSKTLSPPY
                     SILAIGDPPTLAAAMNIPGGAEDSIKRVGARMSVQQADRVDVTTLRQPKPHQYAQPVK
                     "
     gene            7674..8072
                     /gene="gcvH"
                     /locus_tag="VFI55_07845"
     CDS             7674..8072
                     /gene="gcvH"
                     /locus_tag="VFI55_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET7073976.1"
                     /translation="MSDIPADLHYTAEHEWVRRSGDDTVRVGITDFAQSALGDVVFVQ
                     LPDAGSAVTAGESFGEVESTKSVSDLYAPVTGTVSAVNGDLEGSPQLVNSDPYGAGWL
                     LDIQVSATAELESAISTLLDAEAYRGTLTE"
     gene            8380..8862
                     /gene="garA"
                     /locus_tag="VFI55_07850"
     CDS             8380..8862
                     /gene="garA"
                     /locus_tag="VFI55_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen accumulation regulator GarA"
                     /protein_id="HET7073977.1"
                     /translation="MTDMDSEREQDQTSDEVTVETTSVFRADFLNELDAPAQAGTEST
                     VSGVEGLPAGSALLVVKRGPNAGSRFLLDQATTSAGRHPDSDIFLDDVTVSRRHAEFR
                     LENNEFHVVDVGSLNGTYVNREPVDSAVLANGDEVQIGKFRLVFLTGPKHDDDGGSGG
                     "
     gene            8862..9623
                     /locus_tag="VFI55_07855"
     CDS             8862..9623
                     /locus_tag="VFI55_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7073978.1"
                     /translation="MGAPDSSALAGMSIGAVLDLLRPDFPDVTISKIRFLEAEGLVTP
                     QRAASGYRRFTAYDCARLRFILTAQRDHYLPLKVIRAELDAQPDGALPPIGSPYGVPR
                     LVTVSDSGESSVRAGSGTGSDTAAVAPTRIRLSREDLLERAGVDDELLTSLLKAGVIT
                     TGPGGFFDEHAVVILQCAHALSEYGVEPRHLRAFRSAADRQSDLIAQIAGPVVKAGKA
                     GARDRADDLAREVAALAITLHTSLIKSAVRDVLHR"
     gene            9740..10234
                     /locus_tag="VFI55_07860"
     CDS             9740..10234
                     /locus_tag="VFI55_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HET7073979.1"
                     /translation="MGEVRVVGIRVEQPQNQPVLLLREANGDRYLPIWIGQSEAAAIA
                     LEQQGVEPPRPLTHDLIRDLIAALGHSLKEVRIVDLQEGTFYADLIFDRNITVSARPS
                     DSVAIALRVGVPIYVEEAVLAQAGLLIPDESDEETGTAVREDEVEKFKEFLDSVSPDD
                     FKAT"
BASE COUNT         1872 a         3409 c         3454 g         1646 t
ORIGIN      
        1 cgcacgcggc gcgggcagcg caggcgcggc gcgacgggta gcgcgcggcc ggcttcggcg
       61 ccgcccggct gaacgagccg gcccgcctca gacgcgcacg ggctccttct ccacggggcc
      121 cagccgccac tggccgtcgc ccagcagtcg aagttgccgg gtgtgatgct gctcgacggc
      181 gcgccggtgg ctgacgctga cgacgacaca gtccggcagt tcgctgcgca gcaactcgta
      241 cagcgcgaat tccaggcctt cgtcgagcgc ggacgtggct tcgtcgagaa acaccgcctt
      301 gggtttggtg agcaggatgc gggcgaacgc gacgcgctgc tgctcgccgg gggaaagcac
      361 cttggcccag tcgcgctctt cgtcgaggcg gtcgcacagc ggggccagcg ccaccttcgt
      421 cagcgtgtcg cgaagggtgc cttcggcgat gtcgtccggt gaattcgggt agcacaccac
      481 gatgcgcaga ctgcccagtg gcacatacgg caactgcgac aagaacatcg tcgcgttctc
      541 gccgtcgggg cggcacaggg ttcccgacgc gtacggccac aattcggcca ggctgcgcag
      601 cagcgtcgtc ttaccggcac ccgaccgccc ggtgatcacc agcgattcgc cgtgatccaa
      661 ctgcacgtcc agggagtcga tcagctggtc acccgccggt gtgcgcactt cgacgtgggc
      721 gagctccacg gccacctctt cactcggctt gaccagcaac gacggcagcg cgcgcccgcg
      781 agcgtcggca tcgacgagac cgtgcaaccg gatgatcgcg gcgcggaagg ccgcgaacgc
      841 gtcgtagttg ttgcggaaga acgacagcga gtcatggatg ttgccgaatg ccgtcgcggt
      901 ttggccgaca tcaccgaagt cgatctggcc ggcgaacaag cgcggcgcct ggatgatcca
      961 cggcagcggg acgatcgcct gcgacaccga ccagttccat ccgttgaaga tgatggttcg
     1021 gcggacgaac ttgcggtagt taccgatgat cggggtgaag cgccgccaca gttgcgcacg
     1081 ctcgacgcgc tcaccgcgat agaagcccac cgcctcggcc gcgtcccgca gccgcaccag
     1141 ggcataacga aacgccgcgt tcagcttctc gttgttgaaa ctgagccaaa tcagcggacg
     1201 ccccaaccag accgcgacca ccgtcgcgat cagcacgtag accagaacag tccagaacat
     1261 cgcgcgcggg aagttgacgc cgaacaggtt cagatcgccc gacaggtccc acaaaatcgc
     1321 ggcgaacgaa atcaccgagg ccaccgcgtt gacggcgccg aagagcaagg tgcttcccgt
     1381 cccgttggag gggatgttcg gggtgccgcc cgcgttcgcg gtgaagatgt cgacgtcctg
     1441 ctgaatgcgc tgatccgggt tgtcgatggt gttgtcgatg aaccgatcgc ggtagtaagc
     1501 gcggccgtcc agccagtcgt cggtgaggtg ggcggtcagc cacacgcgcc aggcgatgat
     1561 gaatcgctgc gtcagataga tgtcggccat gaaccggatg acgtacagga tggccagcag
     1621 gctgaagatc accagcgaca tccagaaccc gtgaattcca gactctttga ccctggcgtt
     1681 gccggccgcg acgccctgga ccgctttctg caccgccgtg tacaggtcgt tgccctggta
     1741 gctgagcaac acggtgagac gcaccgacag gagcaccgaa aacagcagga cgccgagcac
     1801 cgcccacacc ttgacgctcg cccgaccgac gaagtatccg cgagtgatcc gccagaactg
     1861 ccggccccag ggcgtgagat acctgaaggc gaccagcacg gccaacagag caatggcccc
     1921 gatcacccac gcgatggcga tccaccgcaa ggaatcagtg aatgccgtgg accagttgat
     1981 cgatggcttg aacggcgtgg gacccaactt cgacgtctcc ttgctaccga ggcatccggc
     2041 gagcctcttg gtggatactt ccccgcgaac gtacccgagg cttatagcca ggctgcccgg
     2101 atgagccgcg acgctccgcc ccgccagacc gtgcatgccg ggccacgcat cgctcgccgg
     2161 acgcagagcc ggcggtgccg actccaatcc gcgacaggca ccgtgacgag gttatccggc
     2221 cgatcgacac ccggcacgag cagaccgcga tgccgcgcaa ttgcgcgcca gggctcggct
     2281 tgtcgcgggc aaacacgcgc cctcgtgctg gatgtgaggt cgcgtaccgt tgacctgtgt
     2341 caaagtccac ccgcacgcaa cctggtcgcc tgagtagccg attttggcgc ctgctgggcg
     2401 ccactacgga aaagaaccag agccggtcgc tcaccgaggt cactaagtct tcggaatacg
     2461 acgacgaagc cgccggcctc agcgacgagc aactgcgcaa ggcggccggg ctgctgaaac
     2521 tcgaggatct cgccgactcc gacgacatgg cgcagttcct ggcgatcgcc cgggaagcgg
     2581 cggaaagagc gaccacgcta cgtccatttg acgtgcagat gctgggcgcg ctgcggatgc
     2641 ttgccggcga cgtgatcgag atggccacgg gtgagggcaa aaccctcgcc ggggccatcg
     2701 cggccgccgg gtacgccctt gcagggcgac acgttcacgt ggtgaccatc aacgactacc
     2761 tggcccggcg cgacgcggag tggatgggcc cgctggtgga ggccatgggc ctgacggtcg
     2821 gctggatcac cgcggagtcg acgagcgagg atcgtcgggc cgcctatgac tgcgatgtca
     2881 catacgcctc ggtcaacgag atcggcttcg acgtgctgcg cgatcagctg gtgaccgacg
     2941 tcgccgatct ggtgtcaccc aatcccgatg tggcgctgat cgacgaggcc gactcggtgc
     3001 tggtcgacga ggcgctggtg ccgctggtgc tggccggcac cacgcaccgc gaaacgccgc
     3061 ggctggagat catcacgctg gtgggggagc tggtcggcga caaggacgcc gatgagtact
     3121 tcgccaccga ctccgacagc cgcaacgtcc acctgaccga cctgggcgcg cgaaagctcg
     3181 agaaggcgct cggtggcatc gacctgtact ccgaggagca cgtcggcacc acgctgaccg
     3241 aggtcaacgt cgcgctgcac gcgcacgtgc tcctgcagcg cgacgtgcac tacatcgtgc
     3301 gggacgacgc ggtgcacctg atcaacgcct cgcgcggccg catcgcgcaa ctgcaacgct
     3361 ggccggacgg cctgcaggcc gccgtcgagg cgaaggaggg catcgagacc accgagacgg
     3421 gcgaggtgct ggacaccatt acggtgcagg cgctgatcaa ccgctacgcc accgtgtgcg
     3481 gcatgaccgg caccgcgctg gccgccggcg agcagctgcg ccagttctac aagctcggtg
     3541 tttcaccgat tccgcctaac acccccaaca ttcgcgagga cgaatccgac cgggtctata
     3601 tcaccgccgc cgccaagaac gacgcgatcg tcgcgcacat cgccgaggtg catgagaccg
     3661 ggcagccggt gctggtcggc acccgcgacg tggccgaatc cgaggagttg cacgagcggc
     3721 tgctgcgccg gggcatcccc gcggtcgtgc tcaacgcgaa gaacgacgcc gaggaggcaa
     3781 aggtgatcgc cgaggccggc gagttcggga ccgtcacggt gtcgacccag atggccgggc
     3841 gcggcaccga catccggctg gggggatccg aggaggccga ccacgacagg gtcgccgaac
     3901 tgggcggcct gcatgtggta ggcacgggtc ggcatcacac cgagcggctc gacaaccagt
     3961 tacgcggccg ggccggccgc cagggcgatc cgggatcgtc ggtgttcttc tccagttggg
     4021 aggacgacgt cgtcgcggcc aacctcgacc agaacaagct gccgatgcag accgacgagg
     4081 acggcaagat cgtcagcccc aaggcggccg ggctgctcga ccatgcccag cgggtcgccg
     4141 aggggcggat gctcgatgtg cacgcgaaca cctggcgcta caaccagctg atcgcccagc
     4201 agcgcgccat catcgtcgat cggcgaaaca cattgctgcg caccgcaacc gcccgcgaag
     4261 agctggccga attgacgccg aagcgataca aggagctgtc caagcagctg tccgaagaac
     4321 gcctcgagac catttgccgg ctgatcatgc tgtatcacct ggaccgcggc tgggccgatc
     4381 atctggcgta tctggccgac atccgggaaa gcattcacct gcgcgcactg ggccggcaaa
     4441 acccgctcga cgagtttcac cggttggcgg tcgacgcctt cgcgtcgctg gccgccgact
     4501 cgattgaggc ggctcaacag acgttcgaaa ccgcaaacgt cctcgacgag gagccggggc
     4561 tggacctgtc caaactggcg cgcccgacgt cgacgtggac ctacatggtc aacgacaacc
     4621 cgctgtcgga tgacacgctg tcgacgttga gtctgcccgg cgtgttccgc tagacggccg
     4681 ccgcgtacgc ggccgagcgt gcggctggcc acacgctcga acgttcccgg tccgcgctgc
     4741 ggacctcatc gtcgccgagc taaggtcacg gctcatggag caggtgcctc cgcccgaccg
     4801 cgtgctgaca gtacccaacg tgctgagcgc catccgcctg gtgctcatcc cggtgttcgt
     4861 ctacgcgctg ctgttcgcac acgccaacgg ctgggcggtg gcgattctca tgttcagcgg
     4921 tgcctccgac tgggccgacg gcaagatcgc gcggacgctg aaccagtcgt cgcggctggg
     4981 cgtcctgctg gacccggcgg tcgatcggct ctatatggtc accgtgcccg tcgcgatggc
     5041 ggtggacggg atcgtgccgt ggtggttcgt catcatgctg ctggctcgcg acgggctgct
     5101 ggccgcgacg ttgcccgtgc tgcgcagtcg cgggctgtcg gcgctgccgg tgacctactt
     5161 cggcaaggcc gccactttcg ccctgatggc cggcttcccg ctggtcctgt tgggcacctg
     5221 ggacgcgtcg tggagccgca tactgagagc tgttggttgg ggctttttgg gttggggtat
     5281 gtacgcgtac ctgtggtcgt tcgtgctgta cgtggtgcag atggtgttgg tcgtgcgccg
     5341 aatgcccaag gtgcagccgg taccgggtga gcatggctga cgcggaccgc ctgctcggcg
     5401 gctatgaccc caacgccggc cgcagcgccc acacggcggc ccggccgacc ctgattccgg
     5461 tgccgtccct gctgcgcgcc ttgctgtcgg agcacctcga tcccggttac gcggccgcgg
     5521 cggccaaacg tagcggcccg atggcggcgc ggactgggcg cgagcgcgct gtcggctggt
     5581 tctggcaggc tctggcggca ctgctgatcg ccacggtatt tgccgccgcg gtcgcgcagg
     5641 cccgctcagt tgcgcccggt gtgcacactg cccaggagct gctggcacga agtgtgcggt
     5701 cgacggaagc gtctgcggcc aagttgggcc agcagcgcag cgcgctgtcg acccgggtgg
     5761 acgacgtgca gcggcacgcg ctggcagacg acgccgaggg gcagcgcctg ctgaaccgcc
     5821 tcgacgcgct cagcctggca gcggccagca caccggtcac cggcccgggt ctgacggtga
     5881 ccgtgaccga tcctggtgtc agcccgaatc tttccgacgt ttccaagcag cgcgtcagcg
     5941 gtagccggca aatcatcctg gaccgcgatc tgcagctggt cgtcaactcg ttgtgggcga
     6001 gcggcgccga ggcgatctcg gtcggtggcg tcaggatcgg gccgaacgtg accatccgac
     6061 aggccggcgg ggcgatcctg gtcgacaaca accccaccgg cagcccctac acgatccttg
     6121 cggtcgggcc gccgcacgcc atgcgcgaca ccttcgacca cagccccggg ttacagcgcc
     6181 tcaggctcct cgaggcctcc tacggcgtcg tcgtcagcgt caacgtcgga gacagcctct
     6241 cgctgcccgc cggatcggtc cgggatgtca agttcgccaa acagattggg ccgtagtgag
     6301 aaagctcctc gtaggacaga tccccgtggg ggaaatgcgg acgggaatcc gagcgtgatc
     6361 ggcatcgcag cgcttgcggt cggcatcgtg ttgggcttgg tttttcaccc cgccgtcccc
     6421 gaggtcatcc agccctatct gcccatcgcg gtggtggccg cgctggacgc ggtattcggc
     6481 gggctgcgcg cttacctgga acggatcttc gacccgaagg tcttcgtgat ctcgttcgtg
     6541 ttcaacgtct tcgtcgcggc gctgatcgtc tatgtcggcg accaactcgg cgtcggcacc
     6601 caattgtcca cggccatcat cgtcgtattg ggtatccgca tattcggcaa tgccgccgcg
     6661 ttgcggcgcc ggcttttcgg agcatgacgc gcgccggcga cgatgcagag cgaagcgatg
     6721 aagaggagcg gcgccatgac ggagatgagg tcaccgtgag ccaggacccg cccgatcgcg
     6781 ccggcgaccc agaaagcgaa agccgcgacg caccgatccg gcacggtcac cccgacgctg
     6841 attccacagc ggacgacggc gcgcagcatg gccgccacga actgcccgcg aaacgtccgc
     6901 gccccgagat cgggccggtg catcgaaccg gactgtccgc actgctgcag ggcggtcgcg
     6961 cccgtctggt cttcggaacg ctggccatcc tgttgtgcct ggtgttgggg atcgccattg
     7021 ccacccaggt ccgccagacc aagtcgggcg attcgctgga taccgagcgc cccgcagacc
     7081 tgctggtgct gctggattcg ttgcggcagc gtgaagccac actcaacacg gaagtgagcg
     7141 aacttcagaa cacactgaat tcgctgcagg catccggcaa caccgatcag gccgccatcc
     7201 atagcgcgca ggccaggctg gctgcgctgt ccatcctggt cggctcggtg ggcgccaccg
     7261 ggccgggcgt cacccttacg atcgacgacc cggggcccgg ggtgtcaccg gaagccatgc
     7321 tcgacgtgat caacgagttg cgtgccgccg gcgccgaagc catcgagatc aacgacgccc
     7381 atcagtccgt ccgggtaggt gtcgatacct ggatagtggg tacgcctggc acgctgacca
     7441 tcgacagcaa gacactgtcg cccccgtatt cgattctggc gattggggat ccgcccacac
     7501 tggcggccgc catgaacatc cccgggggcg cagaagacag catcaagcgt gtcggcgcgc
     7561 gaatgtcggt gcagcaggcc gaccgtgtag acgtgaccac cttgcgacaa ccaaaaccgc
     7621 accaatacgc tcagcccgtc aagtgaccaa cccagaccag aacaggatta cccgtgagcg
     7681 atatcccggc cgatctgcat tacaccgccg aacacgagtg ggttcgacga agcggtgatg
     7741 acaccgtgcg ggtcgggatc acggatttcg cgcaatccgc actcggggat gtcgttttcg
     7801 ttcagctgcc cgacgcgggc agcgcagtga ccgcgggcga gtccttcggc gaggtcgaat
     7861 cgaccaaatc ggtgtccgac ctctacgcgc cggtcacggg caccgtatcg gcggtcaacg
     7921 gcgatcttga aggcagcccg cagctggtca actccgaccc atacggggcc ggttggttgc
     7981 tggatatcca ggtttcggct accgccgaac tcgagtcggc catttcgaca ctgcttgacg
     8041 ctgaggccta ccgcggaaca ctgaccgaat gacgattgct aatgtgcctg ccagcctcgc
     8101 ccgccgaagc tggtccaaca cgggggtggg gctgcccgag tacaagtggg tgccgtcgcc
     8161 gtccactggc ggtggaagcc cgacaaacgg gcctccgacc cgcctgtggt gggagcattg
     8221 cggaccagcg cgcggtacgg tcgacacatc agcttcaaag agcacgagaa acgcaagtaa
     8281 tcagcagtac agggctggta ataatggcca gcaagcgagc ggaaaaccgg ccgaccggcc
     8341 cggtcagata gcgccacagc ggccagtgag gagcagcgcg tgacggacat ggactcagaa
     8401 agagagcagg accagacttc tgacgaagtt accgtggaga cgacttcggt cttccgcgcc
     8461 gacttcctca acgaactgga cgctccggcg caagcaggga ccgagagcac ggtatccggg
     8521 gtcgagggac tcccggccgg ctcggcgttg ctggtcgtca aacgaggacc gaatgccgga
     8581 tcgcggttcc tgctcgacca ggccaccacg tccgccggtc ggcaccctga cagcgacatc
     8641 ttcctggatg acgtcaccgt cagccgtcgc cacgctgaat tcaggttgga aaacaacgaa
     8701 tttcacgtcg tggacgtcgg tagcctcaac ggcacctacg tcaaccgcga gcccgtggat
     8761 tcggcggtgc tggcaaatgg cgacgaggtg cagatcggca agttccgctt ggtgtttttg
     8821 accggaccca agcatgacga cgacggagga tccggaggct agtgggcgca cccgatagct
     8881 cggcgctcgc cgggatgtcg attggggcgg tcctggacct gttgaggccg gacttccccg
     8941 atgtcacgat ctccaagatt cgcttcttgg aggccgaagg actggtgaca ccccagcgcg
     9001 ctgcgtcggg ataccggaga ttcaccgcgt acgactgcgc acggctgcga ttcattctca
     9061 ccgcccagcg ggatcactac ctgccgctca aggtcatcag ggcggagctg gacgcccaac
     9121 cggacggcgc cctgccgccc atcggatcgc cttacggtgt accgcgattg gtgacggtgt
     9181 cggacagcgg ggaatcgagt gtccgggccg gatccgggac cggatccgat acggccgcag
     9241 tggccccgac ccgcatccgg ctgagccgag aagatctcct cgagcgcgcc ggagtggacg
     9301 acgaactgct gacgtcgcta ctcaaggcgg gagtgatcac caccggcccg ggcggtttct
     9361 tcgacgaaca cgccgtcgtg atcctgcaat gcgcgcacgc gctgtccgaa tacggcgtcg
     9421 agccgcgcca tctgcgcgcc ttccgctcgg cggccgacag gcagtcggat ctgatcgctc
     9481 aaatcgccgg gccggtagtc aaagccggca aggccggcgc ccgcgatcgt gccgacgacc
     9541 tggctcgcga ggtggcagca cttgccatca cgctacacac ttcattgatc aagtctgcgg
     9601 ttcgcgacgt tcttcaccgg tgaggattag actcgttgga cagcttggtg ttcggcggcg
     9661 tggaagacac aatgttcgac cgcgtcgccg cgccgagtct gagtcagacc acggcagcat
     9721 ttgcggaggg cagacacaaa tgggtgaagt tcgtgtggtc ggcattcggg tagagcagcc
     9781 gcagaaccag ccggtgctgt tgctgcgcga agccaatggt gaccggtact tgccaatttg
     9841 gatcggtcag tctgaggcgg ccgccatcgc gctcgagcag caaggcgtcg agccacctcg
     9901 cccgctgacg cacgatttga tcagggatct cattgcagcg cttgggcatt cgctgaaaga
     9961 ggtgcggatc gtcgatctcc aggaaggcac tttttacgct gacctcatct tcgaccgcaa
    10021 catcacagtg tcggcgcggc cctccgattc ggtcgcgata gcgctgcgcg tcggcgttcc
    10081 gatctacgtc gaggaagccg tgctggccca ggccgggctg ctcattcccg acgagagcga
    10141 cgaggagacc ggcaccgccg tccgggagga cgaggtggag aagttcaagg agtttctcga
    10201 cagcgtgtct cccgacgatt tcaaggccac ctagcccgcc gacgatgcag cccgcgcagc
    10261 ggggtgagaa ggaggcgggc gatccagcct gagcccgccg acgatgcagc ccgcgcagcg
    10321 gggtgagaag gaggcgggcg atccagcctg agcccgccga cgatgcagcc cgcgcagcgg
    10381 g
//
LOCUS       DASSGQ010000178         2148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_43542, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000178 DASSGQ010000000
VERSION     DASSGQ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43542"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VFI55_07865"
     CDS             <1..925
                     /locus_tag="VFI55_07865"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET7073980.1"
                     /translation="AGAIPVIRPLTQSLAGDTVLRVAGIVNGTTNYILSAMDSTGADY
                     DSALADASALGYAEADPTADVEGYDAAAKAAILASIAFHTRVTADDVYREGITKITPA
                     DFVSARALGTTIKLLSICERVTSDDGQERVSARVYPALVPLSHPLATVSGAFNAVVVE
                     AEAAGRLMFYGQGAGGAPTASAVTGDLVMAARNRVLGSRGPKESRYAQLPIAPMGLVS
                     TRYYVSMNVADKPGVLSSVAAEFAKREVSIAEVRQEGVVDEGGRRVGAKVVVVTHTAT
                     DAALSETVDALSDLDVVQSVTSVLRLEGASL"
     gene            922..2004
                     /gene="thrC"
                     /locus_tag="VFI55_07870"
     CDS             922..2004
                     /gene="thrC"
                     /locus_tag="VFI55_07870"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET7073981.1"
                     /translation="MSAPRTAIHRPWPGVIAAYRDRLPVGDDWIPVTLLEGGTPLIAA
                     TRLSKMTGCTVHLKVEGLNPTGSFKDRGMTMAVTDALARGQQAVLCASTGNTSASAAA
                     YAARAGITCAVLIPQGKIAMGKLAQAVMHGAKIIQIDGNFDDCLELARKMAADFPTIS
                     LVNSVNPVRIEGQKTAAFEIVDALGTAPDVHALPVGNAGNITAYWKGYTEYHQEGLID
                     KLPRMLGTQAAGAAPLVHGKPVSNPETIATAIRIGAPASWTAAVAAQQQSNGRFLAAT
                     DEEILAAYHLVAQSEGVFVEPASAASIAGLLKAIDDGWVARGSTVVCTVTGNGLKDPD
                     TALKDMPHVSPLPVDAGLVVEKLGLA"
BASE COUNT          375 a          720 c          722 g          331 t
ORIGIN      
        1 tgcgggcgcc atccccgtca tccgcccgct tacccaatcg ctggccggcg acacggtgct
       61 gcgggtggcc ggcatcgtca acggcaccac caactacatc ctttccgcga tggacagcac
      121 cggcgccgac tacgacagcg cgctggccga tgccagcgca ctgggctatg ccgaggccga
      181 ccccaccgcc gacgtcgagg gctacgatgc cgcagccaaa gccgcgatcc tggcatccat
      241 cgccttccac acccgagtta ctgccgacga cgtgtatcgc gaaggcatca ccaaaatcac
      301 ccccgccgac ttcgtctccg cgcgggccct gggcaccacc atcaaactgc tgtccatctg
      361 cgagcgtgtc accagcgacg atggtcagga acgagtttcg gcgcgcgtct atcccgccct
      421 cgtgccgtta tcgcatccgc tggccacagt cagcggagcg ttcaacgccg tggtggtcga
      481 ggccgaggcc gcgggccggc tgatgttcta cggccagggt gctggcggcg cgcccaccgc
      541 atcggcggtg accggcgacc tggtgatggc cgcccgcaac cgggtcctcg gcagccgcgg
      601 gccgaaagag tccaggtacg cccaacttcc catcgcacca atgggtctcg tctccacccg
      661 ctactacgtc agcatgaatg tcgccgacaa gccaggcgta ttgtcttcag tagcagcgga
      721 attcgccaaa cgtgaggtaa gcatcgccga ggttcgccaa gagggtgtag tcgatgaggg
      781 cggacgacgg gtgggggcca aggtcgtggt ggtcacccac accgccaccg acgcagcgct
      841 ttccgaaacc gtcgatgcgc tgtccgattt ggacgtcgtg cagagcgtga caagcgtgct
      901 gcgactggag ggggccagcc tatgagcgct ccgcggacgg ccatccaccg accctggccg
      961 ggggtgatcg cggcgtaccg cgaccggtta cccgttggcg atgactggat cccggtgacc
     1021 ctgctcgagg gtggtacccc gctgatcgcg gcaacccgac tctcgaagat gaccgggtgc
     1081 acggtccacc tcaaggtcga aggcctcaac cccaccggct ccttcaagga ccgtggcatg
     1141 acgatggccg tcaccgacgc cctggcccgt ggtcagcagg cggtgttgtg tgcgtcgacg
     1201 ggaaatacct cggcgtcggc ggccgcgtat gctgcgcgcg ccggtatcac ctgcgcggtg
     1261 ctgataccgc agggcaaaat cgcgatgggc aagctggcgc aggcagttat gcacggcgcc
     1321 aagatcatcc agatcgacgg aaacttcgac gactgcctgg aattggctcg caagatggcc
     1381 gccgacttcc cgacgatctc gctggtcaac tcggtcaacc cggtgcgcat cgagggtcag
     1441 aaaacggcgg cattcgagat cgtcgacgcg ttgggcaccg cgccggacgt gcacgccctt
     1501 ccggttggca acgccggcaa catcaccgcg tattggaagg gctacaccga atatcaccag
     1561 gaaggtctga tcgacaagct gccccgcatg ctgggcactc aggcggcggg ggcggcgccc
     1621 ctggtacacg gcaagccggt gagcaacccc gagaccatcg cgaccgcgat tcgtatcggc
     1681 gcaccggcgt cgtggacggc tgcggtcgcg gcgcagcagc aatccaacgg gcgcttcctg
     1741 gccgctaccg acgaggagat cctggccgcg taccacttgg tggcccagtc cgaaggcgtc
     1801 ttcgtcgagc cggcatccgc cgccagcatc gcgggcctgc tcaaggcgat tgacgacggc
     1861 tgggtggcgc gcgggtcgac ggtggtgtgc acggtgaccg gcaatggcct caaggatcca
     1921 gacaccgcgc tgaaggatat gccgcacgtg tccccgctgc cggtcgatgc tgggctcgtg
     1981 gtcgaaaaac tggggctggc atagtggcgg cgatcgcaag cgcggcgcag ccgggcgcgg
     2041 cgggtcgccg taatttgggt cgagcggcga tcgcaagcgc ggcgcagccg ggcgcggcgg
     2101 gtcgccgtaa tttgggtcga gcggcgatcg caaacgcggc gcagccgg
//
LOCUS       DASSGQ010000179         3850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_803333,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000179 DASSGQ010000000
VERSION     DASSGQ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803333"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="VFI55_07875"
     CDS             complement(<1..1142)
                     /locus_tag="VFI55_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET7073982.1"
                     /translation="MLTIVMEKARITAADTAPPTAPTQLLGEHVYPDKLDARLLRISG
                     VCILATVMAILDVTVVAVAQRTFIDQFGTTQAVVGWTMTGYTLALATVIPLTGWAADR
                     FGTKRLFMGSVLAFTLGSLLCAVASNIVQLIIFRVIQGIGGGMLLPLGFMILTREAGP
                     GRLGRLMSILSIPMLLAPIGGPILGGWLIGASSWRWIFLINLPIGIITIALAAVVFPR
                     DHPSRSETFDVVGGLLLSPGLATFLFAISSIPGRATLADHHVLVPAIIGLALIAAFVV
                     HALRRADHPLIDLRLFDNRVLTHANVTMLVFAVAFFGAGLLLPSYFQQVLHQTPMQAG
                     VRMIPQGLGAMLTMRLAGPIVDRRGPGRIVLIGIALITAGLGAFALG"
     gene            1395..2099
                     /locus_tag="VFI55_07880"
     CDS             1395..2099
                     /locus_tag="VFI55_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HET7073983.1"
                     /translation="MSGKLIVSVSGIGERTLADVEAFCAQMDARNVPVSLLVAPRLSG
                     DYRLDRDPRTVEWLTARRAGGDAIVLHGYDDPATKKRRGEFAMLRAHEANLRLMAADR
                     VLEHLGLRTRLFAAPGWVVSPGVVKALPQNGFRLLADLHGVTDLVRHTTVRTRVLGIG
                     EGFLTEPWWCRMVVLSAERIARRGGIVRVAVAARQLRKPGPQQAMLDAVDLALMHGCT
                     PTVYRWLSDKAVLDAA"
     gene            2151..3818
                     /locus_tag="VFI55_07885"
     CDS             2151..3818
                     /locus_tag="VFI55_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HET7073984.1"
                     /translation="MSDSSAAGFAADAIIVGAGLSGLVAAGELVERGLRVLILDQENS
                     ANLGGQAFWSFGGLFFVDSPEQRRLGIRDSHELALQDWLGTAAFDRPEDYWPRQWAHA
                     YVDFAAGEKRSWLRARGLKIFPLVGWAERGGYDAQGHGNSVPRFHITWGTGPALVEIF
                     ARQLRDRSTVRFAHRHRVDELIVEGDAVTGVRGTVLEPSAEPRGVSSSRKAVGQFEFR
                     ASAVIVTSGGIGGNHELVRNNWPARMGRVPEQLLSGVPAHVDGRMIAISQEAGARVIN
                     PDRMWHYTEGITNFDPIWPMHGIRIIPGPSSLWLDAAGKRLPVPLYPGFDTLGTLEYI
                     TKSGYDYTWFVLNAKIIEKEFALSGQEQNPDLTGQSVRELLRNRASSGPPAPVQAFVD
                     RGVDFVSANSLRELVTAMNKLPDVAPLDYATIEAEVTARDREVANKFSKDGQITAIRA
                     ARGYLGDRLGRVVAPHRLTDPKARPLIAVKLHILTRKTLGGIETDLEARVLKADGTPL
                     TGLYAAGEVAGFGGGGVHGYRALEGTFLGGCIFSGRAAGRGAANDIA"
BASE COUNT          639 a         1253 c         1322 g          636 t
ORIGIN      
        1 cccagggcga aggcgccaag gccggcggtg atcagtgcga tgccgatgag cacgatcctg
       61 cccggtcccc gtcggtcgac gatcggtccg gccagtcgca tggtgagcat ggcgccgagt
      121 ccctgcggga tcatccgcac cccggcctgc atcggcgtct gatgcagcac ctgctggaag
      181 tagctcggga gcagaaggcc ggcgccgaag aatgccacgg cgaacaccag catcgtcacg
      241 ttcgcgtggg tgagcacgcg gttgtcgaac agccgcagat cgatcagtgg gtggtcggca
      301 cggcgcaacg cgtgcacgac gaacgcggcg atcaacgcca ggccgataat ggccggtacc
      361 agcacgtggt gatcggccag cgtcgcgcgc cccgggatgg acgagatcgc gaaaagaaac
      421 gtcgccaggc cgggcgacag cagcagccca ccgacgacat cgaaggtttc cgaccgtgat
      481 gggtgatctc gtgggaacac gactgccgcg agcgcgatgg tgatgattcc gatcggcagg
      541 ttgatcagga agatccacct ccagctggag gcgccgatga gccaaccacc caggattgga
      601 ccaccgatcg gggcaagcag catcgggata ctcaggatcg acatcagccg ccccagccgc
      661 ccggggcccg cctcgcgggt caagatcatg aatcccaggg gtaacagcat gccgccgccg
      721 attccctgta tcacacgaaa tatgatgagc tgcactatat ttgacgctac tgcgcacagc
      781 agcgagccca gagtgaacgc caacaccgat cccatgaaga gccgtttggt gccgaaccgg
      841 tcggccgccc acccggtcag cgggatcacg gtggccaatg cgagcgtgta gccggtcatc
      901 gtccagccga cgacggcctg ggtggtcccg aattggtcga tgaaggtgcg ttgcgcgacg
      961 gcgacgaccg tgacgtccag gatcgccatc accgtggcca ggatgcacac gccggagatc
     1021 cgcagcagcc gcgcgtccag cttgtcgggg tagacgtgct cgcccagcag ctgagtgggg
     1081 gcggtgggcg gcgcggtgtc ggccgctgtt atacgggcct tttccatgac gatcgttagc
     1141 atatcgaacc gatcatcacc gccgtcgccg gcacacgttt gctggcccac cgtggaccgg
     1201 gcccgcgccg aatcgccggt cgcaccccga acgggcgctt gattgccgcg gcccgacgcg
     1261 cttgcgcggc gggagtatcc gggccgcatc cagggccgcc gaggtgcctt gcggcgtcct
     1321 ggccaggccc gatatacgcc tgctgtctgc gtgtccttca ccatcccgac acctcggatc
     1381 ggcaaactgc gggtgtgtct ggaaaattga tcgtctcggt ctcggggata ggcgaacgca
     1441 ccctggccga cgtcgaggct ttctgcgcgc agatggatgc ccgcaacgtg ccggtgtcgt
     1501 tgctggtggc cccccgactg tccggcgact accggctcga tcgcgatccg cgcaccgtcg
     1561 agtggctgac cgctcggcgg gccggcggcg acgccatcgt gctgcatggc tacgacgacc
     1621 cggccaccaa gaagcgtcgc ggcgaattcg cgatgctgcg ggcccacgag gccaacctgc
     1681 gtctgatggc cgccgaccgg gtgctcgagc acctcgggct acgcacccgg ctgttcgcgg
     1741 ctcccggctg ggtggtgtca cctggcgtcg tcaaggcatt gccgcagaac ggttttcgac
     1801 ttcttgccga tctgcatgga gtgacagacc tggtccggca caccaccgtc cgcacccggg
     1861 tgctgggcat cggcgaaggc ttcctgaccg agccctggtg gtgccgcatg gtggtgctgt
     1921 ctgccgagcg gatcgcccgc cgcggcggca tagtccgcgt cgcagtagcc gcccgtcaat
     1981 tgcgcaagcc cggccctcag caggcaatgc tggacgccgt cgacctggcc ctcatgcatg
     2041 gctgcacacc gacggtgtac cggtggctgt cggataaagc ggttctcgac gccgcctgac
     2101 gtcaggccgt ccgagtggcc tgcggtccgg gggcattacc ctgttcccac atgagcgatt
     2161 cttcggccgc ggggttcgcc gctgacgcga tcatcgtcgg ggccgggctg tcgggactgg
     2221 tggccgccgg cgagttggtg gagcgcggct tgcgggtgct catcctcgat caggagaaca
     2281 gcgccaacct cggcgggcag gcgttctggt ctttcggtgg tctgttcttc gtcgacagcc
     2341 ctgagcagcg ccgcctgggt attcgcgaca gccacgaact cgccctgcag gattggctcg
     2401 gcacggccgc cttcgatcgg cccgaagact actggccgcg ccaatgggcg catgcctacg
     2461 tcgatttcgc ggccggcgag aagcgcagct ggctgcgtgc ccgggggctc aagatctttc
     2521 cgttggtggg ctgggccgag cgcggcggct acgacgctca ggggcatggc aactcggtgc
     2581 cgcgtttcca catcacgtgg gggaccgggc cggcgttggt cgagatcttc gcccgtcaac
     2641 tgcgcgaccg ctccacggtg cgtttcgcgc accgccaccg ggtcgacgag ctgatcgtcg
     2701 agggtgacgc ggtgaccggt gtccggggca ccgtgctgga gccctcggcc gagccacgcg
     2761 gcgtgtcttc gtcgcgaaaa gcggtggggc aattcgaatt tcgtgcctct gcggtgatcg
     2821 tgaccagtgg tgggatcggt ggtaatcacg agctggtgcg caacaactgg cccgcgcgaa
     2881 tgggccgcgt ccccgagcaa ctgctcagcg gggtgcccgc gcacgtcgac ggcaggatga
     2941 tcgccatctc ccaggaggcc ggcgcacggg tgatcaaccc cgaccggatg tggcactaca
     3001 ccgagggcat cacgaatttc gacccgatct ggccgatgca cggcatccgg atcattccgg
     3061 gtccgtcatc gctgtggctg gacgcggccg gtaagcggtt gcctgtcccg ttgtatcccg
     3121 gattcgacac cctgggcacg ttggaataca tcaccaagtc ggggtatgac tacacatggt
     3181 tcgtgttgaa cgccaagatc attgagaagg aattcgccct gtcgggccag gagcagaacc
     3241 ccgatctgac tgggcagagc gtgcgcgaac tgctgcgaaa ccgcgcaagt tccgggccgc
     3301 ccgctccggt gcaggccttc gtcgaccggg gtgtggactt cgtcagcgcg aactcgttgc
     3361 gcgagttggt gaccgcgatg aacaagttgc ccgatgtggc gcccctggac tacgccacga
     3421 tcgaggccga ggtcaccgcc cgcgatcgcg aggtggccaa caaattcagc aaggatggtc
     3481 agatcaccgc aatccgcgcc gcccgtggct acctcggcga ccggctgggc cgcgtggtgg
     3541 cgccgcaccg gctgaccgat ccgaaggcca ggcccctgat cgcggtcaaa ctgcatatcc
     3601 tgacgcgaaa aacgttgggc ggcattgaga ctgacctgga ggctcgggta ctcaaggccg
     3661 acggtacccc gctcaccggg ctgtatgccg ccggcgaggt ggccggattc ggcggcggcg
     3721 gcgtgcacgg ttaccgagcc ctggagggga ccttcctggg cggctgcatt ttctccggac
     3781 gcgccgcggg ccggggcgcg gcaaacgaca tcgcctagcg gcgagcagac gcagaatagc
     3841 cttgcgcgtg
//
LOCUS       DASSGQ010000180         6471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_195864,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000180 DASSGQ010000000
VERSION     DASSGQ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6471
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195864"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFI55_07890"
     CDS             complement(<1..190)
                     /locus_tag="VFI55_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7073985.1"
                     /translation="MIEVYIPATLAMLRQLVTDGSFWPVNGTAFAVTPTLREAYAEGD
                     DEELAEVALREAALASLRL"
     gene            complement(187..1068)
                     /gene="ppk2"
                     /locus_tag="VFI55_07895"
     CDS             complement(187..1068)
                     /gene="ppk2"
                     /locus_tag="VFI55_07895"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HET7073986.1"
                     /translation="MSTATNDGAPAKAKKKRSATPATPVIPNAVYRAELFRLQTEFVK
                     LQEWVRHSGARLVVVFEGRDGAGKGGTIKRITEYLNPRVAPIVALPAPTDRERGQWYY
                     QRYIAHLPAKGEIALFDRSWYNRAGVEKVMGFCTPQEHVLFLRQTPIFEQMLIDDGIL
                     LRKYWFSVSEAEQLRRFRARLNDPVRQWKLSPMDMESLYRWDDYSRAKDEMMVHTDTP
                     VSPWYVVESDIKKHARLNMMAHLLSTIEYYDVERPQVTLPKHPIVSQNYQRPPRELSR
                     YVDDYAATLIGTAGRAE"
     gene            complement(1112..2530)
                     /locus_tag="VFI55_07900"
     CDS             complement(1112..2530)
                     /locus_tag="VFI55_07900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HET7073987.1"
                     /translation="MVTRLSPADASFYRLENTATPMYVGSLSILQRPRAGLSYETLLA
                     TVEKRLPQIPRYRQKVREVRIGMARPVWVDDRDFDITYHVRQLALPSPGSDEQLHDLI
                     ARLAARPLDKSRPLWEMYLIEGLSKNRVALYTKSHQALINGMSALEIGHVIADRTRRP
                     PPLPEDIWIPERDPGSTRLIFGAIGDWLVGPGTQLQAVGSAVLGWVTNYGELLDAGRR
                     VIGVARAVARGTAPSSPLNVTVSRHRRFTVARGSLEDYRTVRARYECDVNDVVLAVVA
                     GALGNWLMSRGVAVSPTATVRAMAPLSIYSDGQLDSTGPGQTISQVTPFLVDLPVGEA
                     NPVVRLSQIAHATESNPTAASLVDARTIVTLSGFAPPTLHAMGIRVATGFPSLSKRTF
                     NLLITNAPGAQSQMYIAGTKLLETYSVTPLLPNQALAISVTSYNGMLYFGINADRDAM
                     SDVDLLPGLLSQSLQELLEGSR"
     gene            2768..3358
                     /locus_tag="VFI55_07905"
     CDS             2768..3358
                     /locus_tag="VFI55_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015291303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3235 family protein"
                     /protein_id="HET7073988.1"
                     /translation="MTIGPAVSPPRRDAFAVTPVVDYEPPRQERRDDPQCAQSSHATR
                     RRRSVRAPHRPYTGQPSRQPKQAAAQVTDVTSAPMRQAAIFADAALRRVLEVIDRRRP
                     AAQLRPLLASSLVDSVRAIAGPVAGPAPGQQGAAVLRRLRLQPAGCHDPETAAEVFGS
                     YSRGERVHAIACRLERVRTDAGDAGTRWLVVALHIG"
     gene            complement(3375..4529)
                     /locus_tag="VFI55_07910"
     CDS             complement(3375..4529)
                     /locus_tag="VFI55_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET7073989.1"
                     /translation="MQVSEALLFQLGALLATLAVFGAVARRFALSPIPVYLLAGLSLG
                     KGGLLSVAAAGDFITTGAPIGIVLLLLTLGLEFSATEFASSLRHHLPSAGVDIVLNAT
                     PGAVAGWLLGLDGVAILSLAGVTYISSSGVIARLLEDLGRLGNRETPAVLSVLVLEDF
                     AMAAYLPLFAVLASRGSWLDAVAGMAAAVGALLAAFAASYRWGHHVGRLVEHPDSEQL
                     LLRVLGITLMVAAVAETLHASAAVGAFLVGLTLTGETAERARKVLSPLRDLFAAIFFL
                     AIGLSVDPHELVPMLPAALALAVVTAATKVATGVFAARRDGVARRGQLRAGTALIARG
                     EFSLIIIGLVGTSIPAVAALATAYVFIMAIAGPVLARYTGGPLPAAGPPG"
     gene            complement(4534..5016)
                     /locus_tag="VFI55_07915"
     CDS             complement(4534..5016)
                     /locus_tag="VFI55_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter regulatory subunit"
                     /protein_id="HET7073990.1"
                     /translation="MDVKEVLLPGVGLRYEFTSHKGDRIGIIARRGGDFDVVLYAPDD
                     PDQARPLLRLTDEEADTVAQILGAPRIAERFTELTREVPGLEAGQVHIRAGSPFVGRP
                     LGDTRARTRTGASIVAIVRDERVLASPGPAETLQERDVLIVIGTENGIAGVEQIVEQG
                     "
     gene            5056..5520
                     /locus_tag="VFI55_07920"
     CDS             5056..5520
                     /locus_tag="VFI55_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7073991.1"
                     /translation="MDGNTVSVERVIKVAPDRIFALLADAGKHASIDGSESVNHASQQ
                     SVPLTMGSKFGMAMRGRRETLFIPYHTTNTVIEYEPDRRIAWKTTALGGLLGGRIWRY
                     ELSPAPAGTLVRETWDVSQDKQKSMITSGSMPRQTEKGMRATLQRIATLLEN"
     gene            complement(5517..6281)
                     /locus_tag="VFI55_07925"
     CDS             complement(5517..6281)
                     /locus_tag="VFI55_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HET7073992.1"
                     /translation="MKRYLTVAYGAAAYLLFLAAFLYLVGFVGNFVVPRTVDHGLPSS
                     LGQAVLVNVLLVGAFGVQHSIMARPAFKQWWTRVVPSSIERSTYVVLSSAVLVLLYWQ
                     WRTMPTVIWDVRQPVGRWAVWVLFWLGWAIALAATFMVSHFDLFGLRQVYLAWRGKPY
                     THIGFHARWLYRLVRHPLMLGFLIAFWAAPTMTAGHLLFAAAMTGYILLATQLEEHDL
                     VAALGDEYRGYRREVPMLLPLRHSPRRHPTATAGQH"
BASE COUNT         1187 a         2168 c         2076 g         1040 t
ORIGIN      
        1 gcagccgcag cgacgccaac gccgcctcac gcagggccac ctcggcgagc tcctcgtcgt
       61 cgccctcggc ataggcttcg cgcaatgtgg gcgtcaccgc gaacgcggtg ccgttgaccg
      121 gccagaacga gccgtcggtg acgagctgcc gcaacatcgc cagggtcgcc gggatgtata
      181 cctcgatcat tcggctcgcc cggctgtacc tatcaaagtc gccgcgtagt cgtcgacgta
      241 cctcgacagc tcacgcggcg ggcgctggta attctggctc acgatcggat gtttcggcag
      301 tgtgacttgg ggccgctcca cgtcgtagta ctcgatcgtc gagagcaaat gggccatcat
      361 gttcagccgc gcgtgcttct tgatatccga ttccactacg taccaggggc tgaccggagt
      421 gtcggtgtgc accatcatct cgtctttggc gcgcgaataa tcatcccacc gatacagcga
      481 ctccatgtcc atcgggctga gtttccattg ccggaccggg tcattcagtc gcgccctgaa
      541 ccggcgcagc tgttcggcct cggagacgga aaaccagtac ttgcgcaata ggatcccgtc
      601 gtcgatcagc atctgttcga aaatcggcgt ctgtcgtaaa aaaagcacgt gctcttgcgg
      661 cgtacagaat cccatgacct tttcgacgcc ggcgcggttg taccacgacc gatcaaagag
      721 cgcgatctcc cccttggcgg gcaggtgggc gatatagcgc tggtagtacc actggccgcg
      781 ttcccgatcc gtcggagcag gcaacgccac gatgggggcg acgcgcgggt tcaggtattc
      841 ggtgatccgc ttgatggttc cgcctttgcc cgcaccgtcg cggccttcga atacgaccac
      901 cagacgcgcc ccggaatgcc gcacccactc ttgcagcttc acgaattccg tttgcagccg
      961 gaataattca gctcggtaga cggcattggg aattaccggt gtggccggcg tcgccgacct
     1021 ctttttcttg gccttcgccg gcgcgccatc gtttgtcgcg gtgctcacaa caacggatga
     1081 tagatccaag caacaatttt cacttcgact cttaccggga accttcgaga agttcctgaa
     1141 gcgattggct caacaggccc ggcagcagat cgacgtcact catcgcgtcg cggtcggcat
     1201 tgatcccgaa atacagcatg ccgttatacg acgtcacact gatcgccaac gcctggttgg
     1261 gcagcaacgg tgtcaccgag taggtctcca gcagtttcgt accggcgatg tacatctgcg
     1321 actgagcccc gggcgcattg gtgatcaaca ggttgaacgt ccgcttggaa aggctcggga
     1381 agccggtggc gactcgaata cccatcgcgt gcagggtcgg tggcgcaaaa cccgacagag
     1441 tgacgatggt cctggcgtcg accaggctgg cggccgtcgg gttggattcg gtggcatgcg
     1501 cgatctgcga cagtcgcacc accggattcg cctcccccac cggcagatcg accaggaacg
     1561 gcgtgacctg actgatcgtt tggccggggc cggtcgagtc aagttggcca tcggaataga
     1621 tcgacagcgg cgccatcgcg cggaccgtcg cggtcggtga caccgcgacc ccgcgcgaca
     1681 tcaaccagtt gcccaatgcg ccggccacca ccgcgaggac cacatcgttg acgtcgcact
     1741 catagcgtgc gcggacggtg cggtagtcct cgaggctgcc ccgagccacc gtgaaccgcc
     1801 gatgacgcga cacggtcacg ttgagcgggc tgctgggtgc cgtgccacgg gccaccgcac
     1861 gggcaacgcc gatgacgcgg cggcccgcat cgagcagctc gccatagttc gtcacccacc
     1921 cgagcaccgc ggacccgacg gcctgcagct gcgtgccggg ccccaccaac caatccccga
     1981 tcgcaccaaa tatcaaccgg gtactgccgg gatcacgttc gggtatccag atgtcctccg
     2041 gcagtggcgg cggacggcgc gtccggtcgg cgatgacatg gccgatttcc agcgcggaca
     2101 tgccgttgat gagtgcctgg tgcgacttgg tatacaacgc gacgcggttt ttggacaggc
     2161 cttcgatcaa gtacatctcc cacagcggcc gcgacttgtc cagcggccgc gcggcgagcc
     2221 gcgcgatcag atcatgcagc tgctcgtcac tgcccggcga gggcagcgcc agctgcctga
     2281 cgtggtaggt gatgtcgaag tcccggtcgt cgacccagac gggcctggcc atgccgatcc
     2341 gcacctcgcg gaccttttgc cggtagcgcg gaatctgggg cagccgcttt tcgacggtgg
     2401 ccagcaaggt ctcgtagctc aatccggcgc gcggtcgttg caggatggac agcgatccca
     2461 catacatcgg ggtggccgtg ttctccagcc ggtagaagga ggcgtccgcg ggggacaacc
     2521 gggtcaccat tgcggccccg tcctccttgt caattcgttc tcatttcgct ctgcggacac
     2581 cgatgtcctg gctcacgcta atcgtccagc cacagcgctg ctgagcgcgg gtacgcgcga
     2641 gctcgagttg tgccatgatg gccactgact gatcaccgtc tgccactctc ctgcaggctc
     2701 ccgattcgga tcgagcaagt ttccctcgtt ttgagttggt tccatcgttt gagttggaga
     2761 gtgcgcgttg accatcggtc ccgcagtgag cccaccccgc cgcgacgcct ttgccgtcac
     2821 ccccgtcgtc gactacgagc cgccgagaca agagcggcgc gatgaccccc agtgcgcgca
     2881 atcatcccac gcaacgcggc gtcgccgcag cgtacgggcg ccgcaccggc cctacaccgg
     2941 ccagcccagc cggcagccaa aacaggcggc ggcccaggtc accgacgtca cctcggcgcc
     3001 gatgcggcag gccgcgatct tcgccgacgc ggcactgcgc cgcgtgctgg aagtcatcga
     3061 ccgccgccgt cccgccgccc agttgcgacc gttgctggcg tccagcctgg tggactcggt
     3121 gcgcgcgatc gccggtccgg tagccggacc ggcgcccggt cagcaaggcg ccgccgtgct
     3181 gcgccggctg cgcctgcagc cggccgggtg ccacgatccg gaaaccgccg ccgaggtttt
     3241 cggttcctac agtcgcgggg agcgggtcca tgccatcgcc tgccggctgg aacgagtgcg
     3301 caccgatgcc ggagatgcag gtacccggtg gctggtggtg gccctgcaca tcggctgacg
     3361 ggcggcggtg tctcctagcc cggcggtccg gccgcgggta gcgggccgcc cgtatagcgg
     3421 gccagcaccg ggccggcgat cgccatgatg aagacatacg ccgtggccag cgccgcgacc
     3481 gcggggatgg acgtgccgac caggccgatg atgatcagcg agaactcgcc ccgggcgatc
     3541 agcgcggtgc ccgcgcgcag ctgcccacgc cgcgccacgc cgtcgcgccg agcggcgaat
     3601 actccggtgg ccaccttggt tgccgcggtg acgacggcca gggcaagagc cgccgggagc
     3661 atcggaacca gctcgtgcgg atcaacggag aggccgatgg ccaagaagaa gattgcggcg
     3721 aacaggtccc gcagcgggct cagcaccttg cgcgcccgct ccgccgtctc accggtgagc
     3781 gtgaggccca ccaggaatgc gccgacggct gccgacgcgt gcagggtttc ggccaccgcc
     3841 gccaccatca gggtgatgcc caagaccctg agcaacagtt gctcggaatc gggatgttcg
     3901 accagtctgc cgacgtggtg gccccagcgg taggacgcgg caaacgccgc cagcagcgcg
     3961 ccgaccgcgg ccgccatgcc cgcaacggca tccagccagc tgccgcgcga ggcaagtacc
     4021 gcgaacaacg gaaggtaggc cgccatcgcg aagtcttcga gaaccagcac cgacagcacc
     4081 gccggcgttt cccggttgcc gagcctgccc aggtcctcca ggagccgtgc aatcacaccc
     4141 gacgaagaga tgtaggtgac gccggccagc gacaggatgg ccacgccgtc caacccgagc
     4201 agccagccgg ccaccgcgcc cggtgtggcg ttgagaacga tgtcgacacc ggcggacggt
     4261 aggtggtggc gcaggctgct ggcgaattca gtcgccgaga actccagacc caaggtgagc
     4321 agcagcaaca cgatgccgat gggcgcgccc gtggtgatga aatcaccggc cgcggctacc
     4381 gacagcaggc cgcccttacc cagcgacaga cctgccagca ggtacaccgg aatcggggac
     4441 aacgcgaatc ggcgtgcgac agcgccgaat acggcgaggg tggccaggag ggcgccgagc
     4501 tggaacagca gcgcctccga aacttgcact ggctcaaccc tgctcgacga tctgctcgac
     4561 tccggcgatg ccattttcgg tgccgatgac tatcaacaca tctcgctctt gcagcgtctc
     4621 ggcgggaccc ggcgaggcga gcacacgctc gtcacgcacg atcgcgacga tcgacgcgcc
     4681 ggtacgggtg cgagcacgag tgtcccccaa cggcctgccc acgaacgggc tgccggcccg
     4741 gatgtggact tggcccgcct cgagccccgg cacttcgcgg gtcagttcgg tgaagcgctc
     4801 ggcgatccgc ggcgcgccca gaatctgggc gacggtgtcg gcctcttcgt cggtgaggcg
     4861 caacagcggg cgggcctggt cgggatcgtc aggggcatac agcacgacgt cgaaatcgcc
     4921 gccgcgccgg gcgatgatgc cgatccggtc acccttgtgg ctggtgaatt cgtatcgaag
     4981 accgacaccc ggcagcaaca cctccttgac gtccatggct caatccttcc agcagggcgg
     5041 ggttagtgtg ggcggatgga cggaaacaca gtcagtgtcg agcgggtcat caaggtggcg
     5101 cccgatcgga ttttcgcgct gctggccgat gccggcaaac acgccagcat cgacggttcg
     5161 gagtcggtga atcacgcttc gcagcaatcg gttccgttga ccatggggtc gaaattcggg
     5221 atggcgatgc gggggcgcag ggagacgctg ttcatcccct accacaccac caacaccgtc
     5281 atcgagtacg aacccgatcg ccgcatcgcg tggaagacca ccgcactggg cggtctgctg
     5341 ggcggccgca tctggcgcta cgagctgagc cccgcccccg ccggcaccct ggtgcgggaa
     5401 acctgggacg tctcgcagga caagcagaag tcgatgatca ccagtggttc gatgcccagg
     5461 cagaccgaga agggcatgcg cgccaccctg cagcggatcg ccacgctcct ggagaattag
     5521 tgctggcccg cggttgccgt cgggtgccgg cgcggcgagt gtcttagcgg cagcagcatc
     5581 ggcacctcgc gacggtagcc gcgatattcg tcacccagcg ccgccaccag gtcgtgctcc
     5641 tccaactgcg tggctagcaa gatgtagccc gtcatggcgg ccgcgaagag caggtgtccg
     5701 gctgtcatgg tgggggcggc ccagaaggcg atgaggaaac cgagcatgag cgggtggcgc
     5761 accagccggt agagccaccg cgcatgaaaa cctatgtggg tgtatggttt tccgcgccac
     5821 gccagataca cctggcgcag gccgaacaag tcgaagtggc tgaccatgaa ggtcgcggcc
     5881 agcgcgatcg cccagcccaa ccagaacaac acccaaaccg cccagcgccc gaccggctgg
     5941 cgcacatccc agatgacggt cggcatcgtt cgccactgcc agtagagcag caccagcacc
     6001 gcactcgaca gcaccacgta ggtgctgcgt tcaatcgacg acgggacgac tcgcgtccac
     6061 cactgtttga acgccggtcg tgccatgatg ctgtgctgga caccgaacgc acccaccagc
     6121 aacacgttca ccaacaccgc ctggccgagg gacgacggga gcccgtgatc gaccgtacgc
     6181 ggcaccacga agttgccgac aaaaccgacc aaatacaaga aggccgcgag gaacagcaag
     6241 taagccgccg cgccataagc aacagtcaag tagcgcttca tagcctcagt gtcgaccgca
     6301 tccgcgcctg cgtatatagg tcatctgcct catttttgcg ccgccagctc taggcaattt
     6361 tgactagtcc gtaccggcgt gaccgtttgc ggcatttgag gcatctgcct catgcggccg
     6421 tggcggcagc ggaccgatga tcggccgtga cagggccact tcgagagtgc a
//
LOCUS       DASSGQ010000181        10176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1228580,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000181 DASSGQ010000000
VERSION     DASSGQ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10176
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228580"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..144
                     /locus_tag="VFI55_07930"
     CDS             22..144
                     /locus_tag="VFI55_07930"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HET7073993.1"
                     /translation="MELLEPERIGVTLSEELQLHPEQSTDAFILHHPEAKYFNV"
     gene            293..574
                     /locus_tag="VFI55_07935"
     CDS             293..574
                     /locus_tag="VFI55_07935"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HET7073994.1"
                     /translation="MEQSLAVKTFEDLFAELGDRARTRPAGSGTVAALDGGIHGLGKK
                     ILEEAGEVWLAAEHEPNEALAEEISQLLYWTQVLMIARGLSLDDVYRKL"
     gene            577..1440
                     /gene="hisG"
                     /locus_tag="VFI55_07940"
     CDS             577..1440
                     /gene="hisG"
                     /locus_tag="VFI55_07940"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HET7073995.1"
                     /translation="MLRVAVPNKGTLSEPASEILAEAGYRRRTDPKDLTVIDPVNHVE
                     FFFLRPKDIAIYVGSGQLDFGITGRDLVRDSAAPVRERLSLGFGSSSFRYAGPAGRDW
                     TPADLAGKRIATAYPNLVRKDLAARGIEATVIRLDGAVEISVQLGVADAIADVVGSGR
                     TLSLHDLVAFGEPLCDSEAVLIESADHDSQGGSELGAARDQLVARVQGVVFGQQYLML
                     DYDCPRSVLDKATSITPGLESPTIAPLADPDWVAIRALVPRRGVNEIMDELAAIGAKA
                     ILASDIRFCRF"
     gene            1555..2037
                     /locus_tag="VFI55_07945"
     CDS             1555..2037
                     /locus_tag="VFI55_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003411044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HET7073996.1"
                     /translation="MTHFLVLLMALLIGVVAGLRSLTAPAVVAWAGFLGWINLSHTWA
                     SWMADLITVIVLTVLAVGELVTDKLPKTPPRTAPLGFGARIIMGGFSGAVIGAAWHFT
                     FSSLGAGVVGAVLGTLGGYHARRRLVAARAGQDLPIALLEDAVAILGGFAILAATANL
                     "
     gene            2034..3407
                     /locus_tag="VFI55_07950"
     CDS             2034..3407
                     /locus_tag="VFI55_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HET7073997.1"
                     /translation="MTEQFDAIIVGAGQAGPPLAGRLTSVGQRVAIVERKLIGGTCVN
                     TGCIPTKTLVASAHAAQLARRSAEYGVGTGAISVDMAKVKARKDDVMLQDREGVESWL
                     DGMDGCTVVRGHARFEDPHTIRVNGELLRADRIFLNVGGRAVVPDIPGLSDVQFLTNV
                     SVLELDTLPSHLVILGGSYIALEFAQMYRRFGARVTVVERGPRLASREDEDVSATVRE
                     ILLAEGIDVVVDADDVRVAKLDKGFELTPRAGAAGVVGSHLLVAVGRRPNTDDLGLEA
                     AGVRTDARGYVVVDDQLKTNVDHIWAMGDCNGKGAFTHTSYNDFEIVAANLLDNDPRR
                     VSDRITTYGLFIDPPLGRAGMTVGQVRASGRKALVGKRPMTRVGRAVEKGETQGFMKV
                     VVDADTDQILGAAILGVGGDEAIHAILDIMSAKAPYTTLSRTMHIHPTVSELIPTVLQ
                     EMSPLSD"
     gene            complement(3526..4320)
                     /locus_tag="VFI55_07955"
     CDS             complement(3526..4320)
                     /locus_tag="VFI55_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET7073998.1"
                     /translation="MIGCFYRRLPTEDDWPAFAPTDYTRSNWGPELQHGSPPLALLTK
                     LIEELSAGSGLRVGRLSLDILGAIPVAPLRARAWVERPGSRVRMVVAEMCADRDGGPA
                     RAVARVTAWLLAVTDTADVASDRYPPLVEGPAEPLPAVFADAGGYFDAINWRPQDTDG
                     ESAAVSWFSPLAHIVDTEPASALQRLAAVVDCANGVGAVLDPGRFMFMNTDTVVHLHR
                     LPVGNDFALRARASVGRDGVGVTSAEVFDRDGFIGTSAQTLLIQRR"
     gene            complement(4317..5222)
                     /locus_tag="VFI55_07960"
     CDS             complement(4317..5222)
                     /locus_tag="VFI55_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecB family exonuclease"
                     /protein_id="HET7073999.1"
                     /translation="MTDQPESPTLGPAPGLAPKPALRPALSPSRAADFKQCPLLYRFR
                     AIDRLPEAPSGAQLRGSVVHAALEQLYGLPAAQRGPDAAATLVESAWDRVIAAEPELA
                     GELDPEQRTQLLTEARALLSGYYRLEDPTRFDPQSCEERVEVELADGTLLRGFIDRID
                     VAATGELRVVDYKTGKAPPVARALAEYKAMFQMKFYAVALLRSRGVLPTRLRIIYLAD
                     GQVLDYAPDHDELLRFEKTLTAIWRAIQSAAQTGDFRPNPSRLCDWCAHQQLCPTFGG
                     TPPPYPGWPDHSGAQAVSRPAEPAA"
     gene            5296..6147
                     /gene="trmI"
                     /locus_tag="VFI55_07965"
     CDS             5296..6147
                     /gene="trmI"
                     /locus_tag="VFI55_07965"
                     /EC_number="2.1.1.220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(58)-N(1))-methyltransferase TrmI"
                     /protein_id="HET7074000.1"
                     /translation="MSATGPFTVGERVQLTDAKGRHYTMFLAPGAEFHTHRGSIAHDG
                     VIGLAEGSVVKSSNGAHFLVLRPLLVDYVMSMPRGPQVIYPKDAAQIVHEGDIFPGAR
                     VLEAGAGSGALTCSLLRAVGPEGRVVSYEQRADHAEHARRNVTNFVDLHGELPDNWQL
                     IVSDLADSDLPDGSFDRAVLDMLAPWEVLDAVSRLVTAGGVLIVYVATVTQLSRAVEA
                     LRAQQCWTEPRSWETLQRGWNVVGLAVRPQHSMRGHTAFLIFTRRLAPGAVAPAPLGR
                     KREGRDG"
     gene            complement(6170..6481)
                     /locus_tag="VFI55_07970"
     CDS             complement(6170..6481)
                     /locus_tag="VFI55_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 family protein"
                     /protein_id="HET7074001.1"
                     /translation="MWIGWLEFDVLLGPELGVQSLKQKRSAIRPLVAELQRKFSVSAA
                     ETGSQDLYRRAGIGVAMVSGDRSHAVEVLDAAERLVAAHPEFELLSVRRGLQRSEDWS
                     S"
     gene            complement(6486..7100)
                     /locus_tag="VFI55_07975"
     CDS             complement(6486..7100)
                     /locus_tag="VFI55_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074002.1"
                     /translation="MHRHLSVLLSTATAVAALGLSGCSSNGPRVLSSSSSPVPVASSP
                     VIAAAPPTAPLPAPEALIDVLNRLADPNVPGADKVNLVEGATPESAATLDKFTNALRD
                     NGYLPMTFAASNVAWSDKNPSHVMATINVNTAQANNPHFTFPMEFTPSPPPQGGWQLS
                     RRTAEMLLALGNSPAPSPAPSPAPAPPPAPAPPPPPAPAPAPTG"
     gene            7385..9217
                     /gene="arc"
                     /locus_tag="VFI55_07980"
     CDS             7385..9217
                     /gene="arc"
                     /locus_tag="VFI55_07980"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HET7074003.1"
                     /translation="MGDSERSEAFGNPRETGMSSGDAAELEQLRREAAVLREQLEHAI
                     GSHGSARSARDVHQLEARIDSLAARNSKLMETLKEARQQLLALREEVDRLGQPPSGYG
                     VLLATHHDETVDVFTSGRKMRLTCSPNIELASLRKGQTVRLNEALTVVEAGTFESVGE
                     ISTLREVLADGHRALVVGHADEERIVWLAEPLVAPDLPDGIRGDDQDDDTRPRKLRPG
                     DSLLVDTKAGYAFERIPKAEVEDLVLEEVPDVSYLDIGGLTRQIEQIRDAVELPFLHK
                     DLYREYALRPPKGVLLYGPPGCGKTLIAKAVANSLAKKMAEVRGDDAREAKSYFLNIK
                     GPELLNKFVGETERHIRLIFQRAREKASEGTPVIVFFDEMDSIFRTRGTGVSSDVETT
                     VVPQLLSEIDGVEGLENVIVIGASNREDMIDPAILRPGRLDVKIKIERPDAEAAQDIY
                     SKYLVETLPLHADDLTEFDGDRGACIAAMIEKVVERMYAEIDDNRFLEVTYANGDKEV
                     MYFKDFNSGAMIQNVVDRAKKNAIKSVLDTGQPGLRIQHLLDSIVDEFAENEDLPNTT
                     NPDDWARISGKKGERIVYIRTLVTGKSSSASRAIDTESNLGQYL"
     gene            complement(9227..9832)
                     /locus_tag="VFI55_07985"
     CDS             complement(9227..9832)
                     /locus_tag="VFI55_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074004.1"
                     /translation="MTDLAGKRYGEVLLVTPGEAGPQASVYNSFPLNDCPAELWSELD
                     PQAIATENGAATALLNGPRYWLMNEIEKQPQTMQITKTFGGIDMILQATVLLSSMNPA
                     PFTVNRVSRNTVFVFNAGQEVYELIDPERRRWVMQTWSQVADPNLKREDLPGLASRLD
                     LPDGWTYQPRVLTSELRVDTRTQSAQVLQDNLTNSYSLETD"
     gene            complement(9880..>10176)
                     /locus_tag="VFI55_07990"
     CDS             complement(9880..>10176)
                     /locus_tag="VFI55_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074005.1"
                     /translation="GLIVLTPSLLNEWTHTYKSTATVLGFTLPVPDSLIHMALFLGAL
                     TFMYVSARAAGDAEYRSTFLDPLIDDLQATLIARDRYRGVVGSAHEVDATQVSD"
BASE COUNT         1702 a         3285 c         3505 g         1684 t
ORIGIN      
        1 ctcgaggacc gggtgaagat gatggagctg ctggagcccg agcgcatcgg cgtgacgttg
       61 tcggaggagc tgcagctgca ccccgagcag tcgaccgacg ccttcatcct gcaccacccc
      121 gaggccaagt acttcaacgt ctagaagcgc gccgcgcgag acggcctgaa gccgtcgcga
      181 gtgtgcgtgt ctgtacaacg acacgccgct gagcttgacg atctgtgcac gctcgtagtc
      241 gcgaacttgt cgggcctcgg cacgaaaccg ccgtgacttt gccgggtgcc gcatggaaca
      301 atcgctggcc gtgaagacct tcgaggacct gttcgccgaa ctcggcgatc gtgcccgcac
      361 ccggccggcc ggtagcggca ccgtcgccgc gctggacggt ggcatacatg ggctgggcaa
      421 gaagatcctg gaagaggccg gcgaggtgtg gctggccgcc gaacacgaac ccaacgaggc
      481 gctggccgag gagatcagcc agttgctgta ctggacgcag gtgctgatga tcgcgcgcgg
      541 actgtccctc gacgacgtct atcgaaagct gtgagcatgc tgcgagttgc ggttcccaac
      601 aaaggcacgc tcagcgagcc ggccagcgag atcctcgcgg aggccggtta ccggcgccgc
      661 accgatccca aggacctgac ggtcatcgac cccgtcaatc atgtcgagtt cttcttcttg
      721 cggcccaaag acattgccat ctacgtcggt tcggggcagc tcgacttcgg catcaccgga
      781 cgggacctgg tgcgcgattc cgccgcgccc gttcgcgagc gcctgtcact gggcttcggg
      841 tcgtcgagct tccgctacgc cggcccggcc gggcgtgact ggacgccggc cgacctggcc
      901 ggaaagcgga tcgccaccgc ctatccgaac ttggtgcgaa aagacttggc ggcccgcggt
      961 attgaagcga ccgtcatcag actcgacggg gccgtcgaga tctccgtgca actcggggtg
     1021 gccgacgcga tcgccgacgt ggtgggatcg ggtcgcaccc tgagcctgca cgacctcgtg
     1081 gccttcggtg agccgctgtg tgattcggag gcggtgctga tcgagagcgc cgatcacgac
     1141 agccagggcg ggagcgagtt gggcgcggcc cgcgatcaac tggtggcccg ggtccagggc
     1201 gtggtgttcg gccagcaata cctgatgctc gactacgact gcccacgctc ggtcttggac
     1261 aaagccacgt cgatcacacc gggcctggag tcgccgacca tcgcgccgct cgccgacccg
     1321 gactgggtgg caatccgcgc gctggtgcct cgtcggggcg tcaacgagat catggacgag
     1381 ctcgcggcga tcggcgccaa ggcgattctg gcttccgaca tcaggttttg ccggttctga
     1441 acgagcactg ctgaggcctc ggctgtgtta gcgtccgcct ggggcaaggc tgcccgcgcg
     1501 tgggccgtcc cccctttatg gtcgccgggt tgttcgtccc ccaggagggt ctccgtgacg
     1561 catttccttg ttctgctgat ggcgttgttg attggtgtcg ttgccgggtt gcgttcgctc
     1621 acggctccgg ccgtggtcgc ctgggccggc ttcctgggct ggatcaatct gagtcacacg
     1681 tgggcgtcgt ggatggccga cctcatcacg gtcatcgtcc tgaccgttct tgcggtcggc
     1741 gaactggtga ccgacaaact cccgaagacg ccgccccgga ccgcgccgct cgggttcggc
     1801 gcccggatca tcatgggcgg attttccggc gcggtgatcg gcgcggcatg gcatttcacc
     1861 ttcagctcgc tcggcgcggg tgttgtcggc gcggtgctcg gcaccctggg cggctatcac
     1921 gcccgcaggc ggctggtggc tgcccgcgcc gggcaggact tgccgatagc gctgctggag
     1981 gacgcggtcg cgatcttggg cggattcgcg attctcgcgg cgacggccaa cttgtgacgg
     2041 agcagttcga cgcgatcatt gttggagccg ggcaggccgg gccgcccctg gccggtcggc
     2101 tgacgtcggt cgggcagcgc gtggcgatcg tcgagcgcaa gctgatcggc ggcacctgcg
     2161 tcaacaccgg atgcatcccc accaagacgc tggtggccag cgcgcacgcc gcccagctgg
     2221 cccggcgcag cgcggaatac ggtgtcggaa ccggcgcgat cagcgtggac atggcgaaag
     2281 tcaaggcacg caaagacgac gtcatgctcc aggaccgcga gggcgtcgaa agctggttgg
     2341 acggcatgga cggctgcacg gtcgtccgcg gtcacgcgcg attcgaagac ccgcacacca
     2401 tcagggtcaa cggtgagctc ctgcgcgccg accggatctt cctcaacgtc ggtggtcgcg
     2461 cggtggtgcc ggacatcccg ggactgtccg acgtgcagtt cctcaccaac gtgtcggtcc
     2521 tcgaactcga cacgctgcca tcacatctcg tcatcctcgg cggcagctac atcgcgctgg
     2581 agttcgcgca gatgtaccgg cgcttcgggg cccgggtgac cgtggtcgag cggggtccgc
     2641 ggctggcgtc ccgcgaagac gaagatgtct cggcgaccgt cagggagatc ttgctggccg
     2701 aggggatcga cgtggttgtc gatgctgacg atgtacgggt cgccaagctg gacaagggat
     2761 ttgagctgac tcctcgtgcc ggtgccgccg gtgtcgtcgg aagtcacttg ctggtggcgg
     2821 tgggacggcg gcccaacacc gacgacctgg gtctcgaggc ggccggtgtg cgcaccgacg
     2881 cccgcggcta tgtcgttgtc gacgaccagc tcaagaccaa cgtcgaccac atttgggcca
     2941 tgggcgactg caacggcaag ggcgcattca cccatacctc gtacaacgat ttcgagatcg
     3001 tcgcggccaa tctgctggac aacgacccgc gtcgggtcag cgaccgcatc accacctacg
     3061 ggctcttcat cgatcccccg ctggggcggg cgggcatgac cgtgggccag gtgcgcgcgt
     3121 cgggccgcaa ggcgctggtc ggtaagcggc cgatgacccg ggtcggcagg gcggtggaaa
     3181 aaggtgagac gcaaggcttt atgaaggtcg tcgtggatgc cgacaccgac cagatcctgg
     3241 gcgccgccat cctcggagtc ggcggcgacg aggcgattca cgcgattctg gacatcatgt
     3301 cagccaaagc gccgtacacg acgctgtcgc ggacgatgca catccacccc accgtcagcg
     3361 agctcattcc gacggtgctg caggagatgt cgccgctatc ggactagtgg cgatcgcaag
     3421 cgcggcgtag ccgggcgcgg cgggtcgtca ccattgggct agtggcgatc gcaagcgcgg
     3481 cgtagccggg cgcggcgggt cgccacgatt gggccacacg gcgcgtcacc gtcgctggat
     3541 cagcagcgtc tgggccgaag tcccgataaa cccgtcccgg tcgaacactt cagccgaggt
     3601 cacccccaca ccgtctcgac ccaccgacgc acgggcccgc aaggcgaaat cgttgcccac
     3661 gggaagccga tgcagatgaa cgacggtgtc ggtgttcatg aacatgaacc ggcccggatc
     3721 aagaaccgcg ccaacgccgt tcgcgcagtc caccacggcg gcgagtcgct gcagcgccga
     3781 cgcgggctcg gtgtccacga tgtgggccag cgggctgaac cacgacaccg cggcggattc
     3841 tccgtcggtg tcctgcgggc gccagttgat cgcgtcgaaa tagccaccgg catcggcgaa
     3901 cacagccgga agcggttcgg cgggtccttc caccagcggg ggatagcggt ccgatgcgac
     3961 gtcggcggtg tcggtaaccg ccagcagcca cgcggtcacc cgcgcgacgg cgcgggccgg
     4021 gccgccgtcg cgatcggcgc acatctccgc gacaaccatg cgcacccgcg agcccggccg
     4081 ctccacccag gcccgggcgc gcagtggggc caccggaatc gccccgagga tgtcgaggct
     4141 gagccggccg acccgcagtc ctgatcccgc ggacagctct tcgatcagct ttgtcagcag
     4201 cgccaacggg ggtgagccgt gttgcagttc gggaccccag ttgctgcgcg tgtagtcggt
     4261 gggggcgaag gccggccagt cgtcttcggt gggcagacga cggtagaagc agccgatcat
     4321 gccgcgggtt cggccggacg cgaaacagcc tgcgcgccgg agtgatccgg ccacccgggg
     4381 tagggcggag gcgttccgcc gaaggtcgga cagagctgct ggtgggcgca ccagtcacac
     4441 aaccgcgacg ggttggggcg gaaatcgccc gtctgcgccg cggactggat ggcccgccag
     4501 attgccgtga gcgtcttttc gaagcgcagc agttcgtcat ggtcgggtgc gtagtccagc
     4561 acctggccgt cggccaggta gatgatccgc agccgggtgg gcagcacacc gcgggaacgc
     4621 agtagcgcca ccgcgtagaa cttcatctgg aacatcgcct tgtattcggc gagtgcgcgc
     4681 gcgaccggcg gcgccttgcc ggtcttgtag tcgaccaccc gtagctcgcc ggtggccgcg
     4741 acgtcgattc ggtcgatgaa gccgcgcagc agcgtgccgt cggctagttc gacctccacg
     4801 cgctcttcgc agctttgcgg gtcgaatcgg gtcgggtctt ccagccggta gtagccggag
     4861 agcaatgccc gggcctccgt cagcagttgg gtccgctgtt cggggtctaa ttcgccggcc
     4921 agttccggtt cggcggcgat cacccggtcc cacgcggact ccaccagcgt ggccgccgcg
     4981 tcggggccgc gctgtgcggc gggcaggccg tagagctgct ccagggcggc atgcaccacc
     5041 gacccgcgca gctgcgcccc cgacggtgcc tcgggcaacc ggtcgatcgc ccggaaccgg
     5101 tacaacaagg ggcattgttt gaagtccgcc gcccgcgacg gagacaacgc cggtctgagg
     5161 gcgggtttcg gcgccagccc cggagccggt cccagtgtgg gcgattccgg ctggtcggtc
     5221 atgattgcag cctaggaccg ggcgccgaca accccgggct gcggttgcgg cgagtcggct
     5281 ggcacgctag acggcgtgtc cgcaaccggc ccgtttaccg ttggcgaacg cgttcagctg
     5341 accgacgcca aggggcggca ctacacgatg ttccttgccc cgggggccga gttccacacc
     5401 catcgcggct cgatcgccca cgatggcgtg atcgggttgg ccgagggcag cgtcgtcaaa
     5461 tccagcaacg gcgcccactt cttggtgctg cgcccgctac tggtcgacta cgtgatgtcc
     5521 atgccccgcg ggccgcaggt gatctacccg aaggacgccg cccagatcgt gcacgagggt
     5581 gacatctttc cgggtgcgcg cgtgctggag gccggagccg ggtccggtgc gctgacgtgt
     5641 tcgttgctgc gcgcggtggg gcccgaaggc cgggtcgttt cctacgaaca gcgcgccgac
     5701 cacgccgaac acgcccgtcg caatgtgacc aactttgttg acctccacgg cgagctgccg
     5761 gacaactggc agctgatcgt gagtgatctg gccgactccg acctgcccga cgggtccttc
     5821 gaccgggcgg tcctggacat gctcgcgccg tgggaagtgc tcgacgcggt gtcacggctg
     5881 gtgaccgccg gcggtgtgct gatcgtctac gtggcaacgg tcacccagct gtcgcgcgcg
     5941 gtggaggccc tgcgggccca gcagtgctgg accgaaccgc gctcatggga gacgctgcag
     6001 cggggctgga acgtcgtcgg gttggccgtt cggccgcagc actcgatgcg ggggcacacc
     6061 gcgttcttga tattcacccg acggctcgcc cccggggccg tggccccggc ccctctcggt
     6121 cgcaaacgcg aggggcgcga cggctagcgc cgggcacgcg cgcggcccgc tagctcgacc
     6181 aatcctcgct gcgttgcagg ccccgtcgca ccgacagcag ctcgaactcg ggatgcgcag
     6241 ccaccaaacg ttcggccgcg tcgagcacct cgacggcatg gctgcggtcg ccggaaacca
     6301 tggcgacgcc gatgccggcc cgtcgataca gatcctgcga acccgtctcg gccgccgaca
     6361 cgctgaactt gcgctgcaat tcggcgacca ggggacgaat cgccgatcgc ttctgtttca
     6421 gtgattgcac tccaagctcc gggcccagca acacgtcgaa ctcaagccag ccgatccaca
     6481 tcggatcaac ccgtgggggc cggggccggc gccggtgggg gaggcggtgc gggggccggc
     6541 ggcggcgcgg gagccgggct gggagccgga ctcggtgcgg gcgagttgcc cagggcgagc
     6601 agcatttccg cggtccgccg ggataactgc caaccccctt ggggcggtgg ggagggggtg
     6661 aactccatcg ggaacgtgaa gtgggggttg ttcgcctggg cggtgttgac gttgatcgtg
     6721 gccatcacgt gggacggatt cttgtccgac caggccacgt tgctcgccgc gaaggtcatc
     6781 ggcagatagc cgttgtcccg caacgcgttg gtgaacttgt ccagcgtggc cgcgctctcc
     6841 ggcgttgccc cttcgacgag gttcaccttg tcggcgcccg ggacgttcgg gtcggcgagc
     6901 cggttcagca cgtcgatgag ggcctcgggc gcgggcaggg gagccgtcgg tggggccgcg
     6961 gcgatgactg gactcgacgc gacggggacc ggtgacgagg aggacgacaa gaccctgggt
     7021 ccgttcgacg aacatcccga gagccccagc gccgccacgg cggtggcggt gctcagcaga
     7081 acggacaggt gtcggtgcat ccagtcgcgc cgtgggctat gcgcccacac ctggggtcgg
     7141 gccaacgggt gcaatggaaa tcacaacggc ggctgccgcg ccgccgccgg cgacgccgtc
     7201 cagggccgtg gcgagggctt ttatggccac gagtgtcctt tcaatcggcg cgattcgagg
     7261 cagagattac cagtggggcc tgcgacgcga cttttgaccg catttgtctc cgcacatcaa
     7321 ccggcaatcg ccggtagcgt tgaaggatcc accgcatcat ttcggggcgg gaaaggagcg
     7381 caagatgggt gactcagagc gttctgaagc attcggtaac ccccgtgaaa ctggcatgtc
     7441 cagcggcgat gctgccgaat tggaacaatt acgccgcgag gcagcggtgc ttcgcgagca
     7501 actcgagcat gcgattggat cgcatggcag cgcccgctct gcccgcgatg tgcatcaact
     7561 cgaagctcgt atcgactccc ttgcggcccg caattccaaa ttaatggaaa ctcttaaaga
     7621 agcgcgccaa caactgttgg ccttacggga agaagtcgac cggctcggac agccgcccag
     7681 cggctacggc gtcctgctcg ccacgcacca cgacgagacg gtcgacgtgt tcacgtccgg
     7741 tcgcaagatg cgcctgacat gctcgcccaa catcgaactg gcgtcgctgc ggaagggcca
     7801 gacggtccgg ctcaacgaag ccctgaccgt cgtcgaggcc ggcactttcg aatcggtggg
     7861 tgagatctcc accttgcgcg aggtgctggc ggacggtcac cgggcacttg tggtgggcca
     7921 cgccgacgag gagcgcatcg tctggctggc cgagccgctg gtggccccgg acttgccgga
     7981 cgggatacgc ggcgacgacc aggacgacga caccaggccc cgcaagctgc ggcccggcga
     8041 ctctttgctg gtcgacacca aagccggcta tgcgttcgag cgcatcccga aggccgaggt
     8101 cgaggacctg gttctggaag aggtgcccga cgtcagctac ctggacatcg ggggcctgac
     8161 ccgccagatc gagcagatcc gcgacgccgt ggagctgccg ttcctgcaca aggacctgta
     8221 tcgggagtac gcgttgcgcc cacccaaggg cgtgttgctg tacggcccgc ccggctgcgg
     8281 taagacgctg atcgccaagg cggtggccaa ctcgctggcc aagaagatgg ccgaggtccg
     8341 cggcgacgac gcccgcgaag cgaagtcgta cttcctgaac atcaaaggtc ccgagctgct
     8401 caacaagttc gtcggcgaga ccgaacgcca catccggctg atcttccagc gagcccgcga
     8461 gaaggcgtcg gaaggcacac cggtgatcgt gttcttcgac gagatggact cgatcttccg
     8521 cacccgcggc accggggtct cctcggacgt cgagaccacc gtggtcccgc agctgctctc
     8581 cgagatcgat ggtgtggagg gactcgagaa cgtcatcgtg atcggcgctt ccaaccgcga
     8641 ggacatgatc gaccccgcga tcctgcggcc cggtcgtctc gacgtgaaga tcaagatcga
     8701 gcgtccggat gccgaagcgg cgcaagacat ctactcgaag tacttggtcg agacgcttcc
     8761 gttgcacgcc gacgacctca ccgagttcga cggcgaccgc ggtgcctgca tcgcggcgat
     8821 gatcgaaaag gtcgtcgaac ggatgtacgc cgagatcgac gacaaccgtt tcctggaggt
     8881 cacctacgcc aacggcgaca aggaagtcat gtacttcaag gacttcaact ccggggcgat
     8941 gatccagaac gtcgtcgacc gcgcgaagaa gaacgcgatc aagtcggtgc tggacaccgg
     9001 ccagccgggt ctgcgaatcc agcacctgct ggattcgatc gtcgacgagt tcgccgagaa
     9061 cgaagacctg cccaacacca ccaaccccga tgactgggcg cggatctcgg gcaagaaggg
     9121 cgagcggatc gtctacatcc gcaccctggt caccggcaag tcgtcgagcg cgtcgcgggc
     9181 catcgacacc gagtccaacc tgggccagta cctgtagcgc cctaagtcag tcggtctcca
     9241 gcgagtagct gttggtcaga ttgtcctgca gcacctgggc ggactgggtt ctggtgtcca
     9301 cgcgcaactc gctggtgagc acccgcggct gataggtcca cccgtcgggc agatcgagtc
     9361 ggctggccag cccaggcagg tcttctcgct tcaggttggg gtcggcgact tggctccagg
     9421 tctgcatgac ccagcgcctt ctttcggggt cgatgagttc gtagacctct tggccggcgt
     9481 tgaagacgaa caccgtgttg cggctcaccc tgttgacggt gaagggtgcc gggttcatcg
     9541 aggacagcag gaccgtggcc tgcaggatca tgtcgatccc accgaaggtc ttggttattt
     9601 gcatcgtctg cggctgcttt tcgatctcgt tcatcagcca gtagcgcgga ccgttgagca
     9661 gggccgtggc cgcaccgttt tcggtggcga tggcctgcgg gtcgagttcg gaccacaact
     9721 cggcgggaca gtcgttgagc gggaagctgt tgtacaccga ggcctgcggg ccggcctccc
     9781 cgggggtcac gagaagcact tcgccgtaac gttttccagc caaatcggtc acggggtcac
     9841 acgcttcctc ttcgacgggc ggcagacacc agtgtgaaat tagtcactga cctgggtggc
     9901 gtcaacttcg tgcgcggacc caacgacacc gcgatagcga tcgcgcgcga tcagcgtggc
     9961 ctgcaggtcg tcgatcagcg ggtcgaggaa tgtggagcga tattcggcgt caccggccgc
    10021 gcgggcgctg acatacatga acgtcaacgc gccgaggaag agcgccatat ggatcagcga
    10081 atccggcacc ggcagcgtga atcccagcac cgtcgccgtg ctcttgtacg tgtgggtcca
    10141 ttcgttgagc agcgatggag tgagaacgat cagccc
//
LOCUS       DASSGQ010000182        15137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_712216,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000182 DASSGQ010000000
VERSION     DASSGQ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15137
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712216"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VFI55_07995"
     CDS             <1..777
                     /locus_tag="VFI55_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031344725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET7074006.1"
                     /translation="EVTERDGGFFYPGPDGDIEVFQEFGKIGKSLKNSISPDEICDEY
                     GADTLRVYEMSMGPLEASRPWATKDVVGAHRFLQRVWRLVIDEQTGETRVTHQADQQL
                     DPGTLRSLHRTIAGVSEDYVGLRNNTAAAKLIEYTNHLTKAHRDSVPRAAVEPLVLML
                     APLAPHMAEELWLRLGHATSLAHGPFPEADPAYLVDDTVEYPVQVNGKVRGRVVVGAD
                     ADDETLKAVAMADEKVRAFLAGATPRKVIVVPGRLVNLVV"
     gene            complement(785..1456)
                     /locus_tag="VFI55_08000"
     CDS             complement(785..1456)
                     /locus_tag="VFI55_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7074007.1"
                     /translation="MPTALITGAGGAIGSAIATELSSTHTLLLAGRPSDRLDGVAERL
                     GATTFPLDLTDTDEIAAACEIVDELDVLVHNAGVSIPGHVGESDVDEWRATFAVNVFG
                     AVELTLALLPALRRAGGQVIFINSGAGRTVSPGMASYSASKFALRAFADSLRDDEPEL
                     RVTTIYPGRTDSAMQRELVAFEGGDYDPAKFLRPETVAAAVAGAVATPPDGHVHEVVL
                     RPGRR"
     gene            complement(1503..2006)
                     /locus_tag="VFI55_08005"
     CDS             complement(1503..2006)
                     /locus_tag="VFI55_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7074008.1"
                     /translation="MADSESTSGEVTELAEGLHRALSKLFSILRRGDPNGAAAGELTL
                     AQLSILVTLLDQGPIRMTDLAAHERVRTPTTTVAIRRLEKIGLVKRSRDPSDLRAVLV
                     DITPRGRAVHGESLANRRAALAAMLSQLPESDLNTLTKALAPLERLASGEPTSGPAGE
                     PPARKEA"
     gene            complement(2074..2823)
                     /locus_tag="VFI55_08010"
     CDS             complement(2074..2823)
                     /locus_tag="VFI55_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET7074009.1"
                     /translation="MTADTAGRPSVSLGAKDIHQTLGGTKVLCGVDIEAPAGRTVAVI
                     GPSGSGKSTLLRTLNRLHEPDSGDILLDGRSVLRDDPDELRQRIGMVFQHYNLFPHLS
                     ILKNVSLAPCKLRGLSPDESRELALAQLERVGLKHKAGARPGMLSGGQQQRVAIARAL
                     AMAPQVMFFDEATSALDPEMVKGALGLIADLGAGGMTMVVVTHEMGFARSASNTVVFM
                     DQGKVVECGPPEQIFEAAKTERLQRFLSQVL"
     gene            complement(2820..4634)
                     /locus_tag="VFI55_08015"
     CDS             complement(2820..4634)
                     /locus_tag="VFI55_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET7074010.1"
                     /translation="MNLCPDWRAARRKLFALAATTLIVSGLALAGPTAPASADWNQCA
                     PAGVDSAISLPKDLTETPTMGQDDDHTTATVEPLSAVNVGALGLGTPGVLTVGTLSDA
                     PPNICINPTGQFSGFDNQLLGAIADKLGLQVRFVSTDFAALLAQVASRRFDVGSASVK
                     ATDARRRTVAFTNGYDFGFYSLVVPPGSPIHNFSDLAASQRIGVVQGTVEESYVVDTL
                     HMQPVKYPGFATVYASLKSRQIDAWVAPGTLAATVMRPGDPADVVANTFSRGNFVAYA
                     VAKDNQPLVDALNSGLDAVIADGTWANLYCQWVPRTTPPGWKPGSKAAPTPQLPDFSA
                     IAASQHRPPVGRATPKSTLTQLRDSFFDWDMYRQAIPILFTTGLPNTLILSTSASVIG
                     LALGMVLAMAGMSHSRSLRWPARVYTDIFRGLPEAVIILIIGLGIGPLVGGITHNNPY
                     PLGIAALGLMAAAYIGEILRSGIQSVDSGQLEASRALGFSYGAAMRLVVVPQGIRQVL
                     PALVNQFIALLKGSALVYFLGLVARQRELFQVGRDLNAQTGNLSPLVAAGLFYLLLTI
                     PLTHLVNYIDARMRRGRNAAEPEGHAGIVASTHAQGIT"
     gene            complement(4672..5412)
                     /locus_tag="VFI55_08020"
     CDS             complement(4672..5412)
                     /locus_tag="VFI55_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014710756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7074011.1"
                     /translation="MPKKYGIKEKDQVVSHILNLVLTGKLRSGDRVDRNEIAVGLGVS
                     RVPIQEALVQLEHDGIVSTRYHRGAFVERFDEATVLEHHELDGLHNGIASARAATNPT
                     PRILGQLDALMRSIRSAKESRAFSEITGEYRRTINDEYAGPRLHATIRASQDLIPRAF
                     WMTYQCSRDDMLPFYEDETSAIHRRDPDGARAACIGRSNLMAKTMLAELFRRRVFAAP
                     DDIGPVVPGPIQVFAGADAPHAEPSITL"
     gene            complement(5531..6337)
                     /locus_tag="VFI55_08025"
     CDS             complement(5531..6337)
                     /locus_tag="VFI55_08025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET7074012.1"
                     /translation="MTSPVHPDRPIRIGVQLQPQQAPRYRDLRDAVRRCEDIGVDIAF
                     NWDHFFPLYGDPDGAHFECWTMLGAWAEQTSRIEIGALVTCNSYRNPELLADMARTVD
                     HISGGRLVLGIGSGWKQRDYDEYGYEFGTAGSRLDDLAAALPRITARLAKLNPQPTRD
                     IPVLIGGGGERKTLRLVAEYADMWHSFSSADEFPAKSAVLGRHCADVGRDPAKIERSA
                     AVNGAVGGGGLVANAETLVGLGVTLLTVGCDGPDYDLTATEALCRWRDSR"
     gene            complement(6370..7287)
                     /locus_tag="VFI55_08030"
     CDS             complement(6370..7287)
                     /locus_tag="VFI55_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7074013.1"
                     /translation="MSEDGLAELTEFALLSENAEQAGVAGPVPDVERVQADSAEGTIS
                     ALRWGAPSSERPPRIVFLHGGGQNAHTWDTVIVGLGEPALAVDLPGHGHSAWREDGDY
                     SPQRNAAALAPVLRDLAPTADLVVGMSLGGLTAIRLAAVAPDLVDELVLIDVTPSALQ
                     RHAQLTAEQQGTVALMHGEREFPSLQAMLDLTIAAAPHREVKALRRGVFHNSRRLDNG
                     NWSWRYDAIRTFPDFAGLWDDVDRLAAPITLVRGGLSGFVTDEDAAELGKRATNFRVA
                     HVVDNSGHSVQSDQPRALIDILRGVLDAR"
     gene            complement(7509..8615)
                     /locus_tag="VFI55_08035"
     CDS             complement(7509..8615)
                     /locus_tag="VFI55_08035"
                     /EC_number="5.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HET7074014.1"
                     /translation="MSEHQPLGAPEAHPEVRVAIVGVGNCASSLVQGVQYYLDADENS
                     SVPGLMHVKFGQYHVRDVKFVAAFDVDAKKVGFDLSEAIFASENNTIKIADVPPTNVT
                     VQRGPTLDGIGKYYADTIELSDTEPVDVVQTLREANVDVLVSYLPVGSEEADKFYAQC
                     AIDAGVAFVNALPVFIASDPVWAKKFADAGVPIVGDDIKSQVGATITHRVMAKLFEDR
                     GVQLDRTMQLNVGGNMDFLNMLERERLESKKISKTQAVTSNLQREFKTRDVHIGPSDH
                     VGWLDDRKWAYVRLEGRAFGDVPLNLEYKLEVWDSPNSAGVIIDAVRAAKIAKDRGIG
                     GPVVAASAYLMKSPPKQLADDVARAQLEEFIIDA"
     gene            complement(8684..9226)
                     /locus_tag="VFI55_08040"
     CDS             complement(8684..9226)
                     /locus_tag="VFI55_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET7074015.1"
                     /translation="MLELAILGLLIESPMHGYELRKRLTGLLGAFRAFSYGSLYPALR
                     RMQVEGLIAENAAPAGTPVRRARRVYQLTDAGRRRFGELVADTGPVNYTDDGFGVHLA
                     FFNRTPAEARMRILEGRRRQVEERREGLREALARASSSFDRYTRQLHQLGLESSEREV
                     KWLNELIAAERAAPGLTEQT"
     gene            complement(9402..9710)
                     /locus_tag="VFI55_08045"
                     /pseudo
     CDS             complement(9402..9710)
                     /locus_tag="VFI55_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302368.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9914..10339
                     /locus_tag="VFI55_08050"
     CDS             9914..10339
                     /locus_tag="VFI55_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HET7074016.1"
                     /translation="MRLQRQVVDYALRRRSLLAQVYSGRTGVSEVCDANPYLLRAAKF
                     HGKPSQVMCPICRKEQLTLVSWVFGDHLGAVSGSARTAEELVMLATRFTEFSVHVVEV
                     CRTCSWNHLVKSYVMGALRPPKGTGGTGTARNGARTAIE"
     gene            10371..12914
                     /locus_tag="VFI55_08055"
     CDS             10371..12914
                     /locus_tag="VFI55_08055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET7074017.1"
                     /translation="MNESATERVGRHASNDRPGNPERPVQDGSGSGRRHPVPPDDRLT
                     QILPAVVDGRSPQRTDRIEEVKAALDNPSSTPLRRDALDEVKAALESRAAPPPWRGGP
                     VREHRPPEAPPPPPGPPGGPGGPGGTFPGGHGPRGHGGLADQINWRWVRRLAYIAAAV
                     LVLLPVVTFAMAYFIVDVPKPGNIRTNQVSTILASDGSEIGKIIPPEGNRVDVNLSQV
                     PVHVRQAVIAAEDRNFYSNPGFSFTGFARAVNNNLFGGGDLQGGSTITQQYVKNALVG
                     SAQHGFSGLMRKAKELVIATKMSGEWSKDDVLQAYLNIIYFGRGAYGISAATKAYFDK
                     PVEQLTISEGALLAALIRRPSSLDPAVDPKGAAERWNWVLDGMVETKALSPSDRAAQV
                     FPATVPPDLARAQSQITGPDGLIERQVTKELMELFNIDEQTLNTQGLQVTTTIDPKAQ
                     QAAEKAVSKYLDGQDPDMRSAAVSIDPHNGAIRAYYGGSDANGFDFAQAGLQTGSSFK
                     VFALVAALEQGIGLGYQVDSSPLTVDGIKITNVDGESCGTCNIAEALKLSLNTAYYRL
                     MLKLKGGPQAVAAAAHQAGVAASFPGVPHTLSEDGKGGPPNNGIVLGQYQTRPIDMAS
                     AYATLAASGIYHRPHLVQKVVNAEGQVLFDAGGKDSSGEQRIDKGVADNVTSAMQPIA
                     AYSRGHNLAGGRPSAAKTGTVQLGDSQANKDAWMVGYTPSLSTAVWVGTVQDNVPLVT
                     ASGAEVYGSGLPSDIWKSTMDGALVGTSNESFPKPTEIGGYAGAPVAPPPPPPPPSET
                     VIQPTIEVAPGITIPVGPPTTITAGPPPPGGPQPGGVPPGGPQAPPPPP"
     gene            12911..14569
                     /locus_tag="VFI55_08060"
     CDS             12911..14569
                     /locus_tag="VFI55_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HET7074018.1"
                     /translation="MTGAQQQSETISPLPLAADRRSADNRDCPSRTDFLGAALADVVG
                     GPVGRHALIGRTRVMTPVRVMFVMALVFLALGWSTKAACLQSSGTGTGDQRVANWDNQ
                     RAYYELCYSDTVPLYGAELLNQGKFPYKSSWIESDANGAPQTRYDGQIAVRYMEYPVL
                     TGIYQYASMALAKTYTAVSKVAPLPVVAEVVIFFDVAAFGLSLAWLATVWASAGLSGR
                     RVWDAALVGASPLVIFQIFTNFDALATAFAMGGLLAWARRKPVLAGVLIGLGAAAKLY
                     PLLFLGPMLVLGIRTGRLGALWRTAAAAVTTWLLVNLPVLVLFPRGWSEFFRLNTRRG
                     DDMDSLYNVVKSFTGWRGFDPNLGFWEPPTVLNTVVTVTFAACCAAIAYVVLTAPRRP
                     RVAQLAFLAVAAFLLTNKVWSPQFSLWLVPLAVLALPHRRILLTWMTIDALVWVPRMY
                     YLYGNPNRGLPEQFFTTTVLLRDVAVMVLCALVIREIYRPEEDLVRWGGRVDDPGGGP
                     FDRAPDAPPDWLPDWLRPPALRRAVAEASETDAETEAVAGARHA"
     gene            14569..>15137
                     /locus_tag="VFI55_08065"
     CDS             14569..>15137
                     /locus_tag="VFI55_08065"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET7074019.1"
                     /translation="MLVAIPLFPRFTALDAVGPYEVLQRIPSIDVVFVGHRRGEVRTE
                     NGMLGITCDATFGEVSAPDVVVVPGGIGTRQLLHDEVIRDWFRSVHPHTRFTTSVCTG
                     ALLLAAAGLLDGLTATTHWRAASLLNELGARYVPDRVVEHLPQRLITAAGVSSGLDMA
                     LRLAELLVDRLAAQAAQLLIEYDPQPPFDT"
BASE COUNT         2537 a         4848 c         5090 g         2662 t
ORIGIN      
        1 gaggtgaccg agcgcgatgg cggctttttc tatccggggc ctgacggcga catcgaagtc
       61 tttcaggaat tcgggaaaat cggtaagagc ctgaagaatt cgatatcgcc cgacgagatt
      121 tgcgacgaat acggcgcgga cacactgcgg gtttacgaga tgtcgatggg accattggag
      181 gcatcccgtc cgtgggccac caaagacgtc gtcggcgcgc atcgtttcct gcagcgggtg
      241 tggcggctgg tgatcgacga gcagaccggc gagacacggg tgacccacca agccgaccaa
      301 cagctggacc ccggcacgct gcggtcgctg caccgcacca tcgccggggt ctcggaagac
      361 tatgtgggac tgcgcaataa caccgcggcg gccaagctca tcgagtacac caaccacctc
      421 accaaagcgc accgtgactc ggtgccgcgc gccgcggtcg aaccactggt gttgatgttg
      481 gcgccgctgg ccccgcacat ggccgaggag ctgtggctgc ggctgggcca cgccacctcg
      541 ttggcgcatg gcccgttccc agaggccgac cccgcctacc tggttgacga caccgtcgag
      601 tacccagtgc aggtgaacgg caaggtacgt ggacgcgtgg tggtgggcgc cgatgccgat
      661 gacgagacgc tgaaagctgt agccatggct gatgaaaagg tccgggcgtt cttggcgggc
      721 gccaccccgc gcaaggtcat tgtggtcccc ggtcggctgg tcaacctggt cgtctgaagc
      781 acgatcagcg ccggccgggc cgcagcacca cttcgtggac gtgaccatcg ggcggcgtgg
      841 ccaccgcacc ggcgaccgcc gccgcgacgg tttcgggcct caggaacttt gcggggtcgt
      901 agtcgccccc ctcgaacgcg acgagttcac gttgcatcgc ggaatccgta cgacccggat
      961 agatcgtagt cacccgcagc tctggctcgt cgtcgcgcag tgagtccgca aaggcgcgca
     1021 gcgcgaactt gctggccgaa taggaggcca tgcccggcga aacggtgcgc cccgcaccgg
     1081 agttgatgaa gatcacctgg ccaccggcac ggcgcagcgc cggcaacagc gctagcgtaa
     1141 gttccactgc cccaaagaca ttaacggcga aagtggcgcg ccattcgtcg acgtcggatt
     1201 caccgacatg accggggatg gagaccccgg cgttgtgcac cagcacgtcg agttcgtcca
     1261 cgatctcgca agcggcggca atctcgtcgg tgtcggtcaa gtcgagcgga aaagtggtgg
     1321 caccgagccg ttccgcgacg ccgtcgagcc gatcggaggg ccgaccggcc aacaacagag
     1381 tgtgcgtcga cgatagttcc gtggcgatgg cggagccgat agcgccgccc gcgccggtga
     1441 tgagtgcagt tggcatgttc ccgcctagga aagtcacgaa ttccgttgga aatcggacat
     1501 tttcacgcct ctttgcgcgc cggcggctct cctgcggggc ctgaggtcgg ttcgccggaa
     1561 gccagacgtt ccagtggcgc caacgccttt gtcagcgtgt tcaggtctga ctcgggaagt
     1621 tggctgagca tcgcggccag ggcggcgcgg cgattggcca gcgactcgcc gtgaacggcg
     1681 cgcccgcgtg gggtgatgtc caccagcacc gcccgcaaat cggatgggtc gcgggaacgt
     1741 ttcaccagtc cgatcttctc cagccggcgg atcgccacgg tggtggtcgg cgttcgcacc
     1801 cgttcgtgtg ccgccaggtc ggtcatccga atggggccct gatccagcaa tgtgacgagg
     1861 atcgataact gcgcaagcgt caattccccg gccgcagcgc cattggggtc cccgcggcgc
     1921 agaattgaaa atagtttgga cagcgcgcgg tgcaaacctt ccgcaagctc cgtcacctcg
     1981 cccgaggtgg attcgctgtc ggccataagt cggcagtcta accggatatt cggtgcgtac
     2041 aaggtagcta gcgccgagca gacaccgacc ttcttacagc acctgggaca aaaaccgctg
     2101 gagtcgctcg gtcttcgcgg cctcgaatat ctgctcgggc ggcccgcatt ccacgacttt
     2161 gccctgatcc atgaaaacga cagtgttgga cgccgaccgg gcaaaaccca tttcatgcgt
     2221 gacgacaacc atcgtcatgc cccccgcgcc gaggtcggcg ataagcccca gcgcgccttt
     2281 gaccatttcg ggatccagcg ccgaggtggc ttcgtcaaag aacatcacct gcggggccat
     2341 ggccagcgct cgggcgatcg caacgcgttg ttgttggcca ccggacagca tgccgggacg
     2401 ggcaccggcc ttgtgcttca ggccaactcg ctccagctga gccagcgcga gttcccgcga
     2461 ctcgtcggga gacagtcccc gtaacttgca cggcgccaag gagacgtttt tcagaatgct
     2521 gagatgcggg aaaaggttgt aatgctggaa caccatgccg atgcgctggc ggagttcgtc
     2581 gggatcgtcg cgcaagaccg atcggccgtc gagcaggatg tcgccgctgt cgggctcgtg
     2641 caaccggttc agggtgcgca acagcgttga cttgcccgag ccggagggtc cgatcacggc
     2701 taccgtgcgg cccgcggggg cctcgatgtc gacgccgcac agcacctttg tcccgccaag
     2761 agtttgatga atatccttgg ctcccaacga gactgacggt cgtcctgcgg tgtctgcggt
     2821 cacgtgatcc cctgggcatg ggtggacgcg acgatgccgg cgtgcccttc tggttcagcc
     2881 gcgttgcggc cgcggcgcat gcgggcgtcg atgtagttca ccaaatgcgt taacggaatg
     2941 gtcaacagca agtagaagag accggcggca accaacggcg acaggttgcc ggtctgcgcg
     3001 ttcaggtcgc gacccacctg gaacagctct cgctgtcggg cgaccaaacc caggaagtac
     3061 accagcgcgg agcccttcag cagcgcgatg aactgattga ccagtgccgg cagcacctgc
     3121 cgtattccct gcggcaccac caccagccgc atcgcggccc catagctgaa gccaagcgca
     3181 cgggaggctt ccagctggcc ggagtcgacg ctttggatac ccgagcgcag gatctcgccg
     3241 atgtaggcgg cggccatcag cccgagtgcg gcaatgccca gcgggtacgg attgttgtgc
     3301 gtgatcccgc ccaccaacgg gccgatgccc agcccgatga tcaggatgat caccgcctcg
     3361 ggaagaccgc ggaagatgtc ggtatacacc cgagccggcc agcgcaggga gcgtgaatgt
     3421 gacattccgg ccattgccag gaccatgccc agcgccagcc cgatgacgct ggcgctggtg
     3481 gacaggatca acgtattggg caaccccgtg gtgaacagaa tggggatggc ctgtcgatac
     3541 atgtcccagt cgaagaagga gtcacgcaac tgggtcagtg tcgacttcgg cgtggcccgg
     3601 cccaccggcg ggcgatgctg gctggcggcg atcgcggaga agtccggcag ttgcggggtg
     3661 ggggcggctt tcgatccggg cttccagccg ggcggcgttg tgcgcggcac ccactggcaa
     3721 tacaggttcg cccaggtgcc gtcggcgatg acggcgtcca gcccggaatt cagcgcgtcg
     3781 accagcggct ggttgtcttt ggcgaccgca taggccacga agttgccgcg gctgaacgtg
     3841 ttggcgacaa cgtctgccgg gtcgcccggc ctcatcacgg tcgccgccag agtccccggc
     3901 gccacccacg cgtcgatctg gcgagacttg aggctggcgt acaccgtggc gaagccgggg
     3961 tacttcacgg gttgcatgtg caaggtgtcg accacatacg actcctcgac ggtgccctgg
     4021 accacgccga tgcgctggct cgccgcgaga tcgctgaagt tgtggatcgg tgagccgggg
     4081 ggcaccacca gcgaatagaa gccgaagtcg tagccattgg taaacgcgac ggtgcgacgc
     4141 cgcgcgtcgg tggctttcac cgacgccgag ccaacgtcga agcgccggga cgccacttgg
     4201 gcgagcagcg cagcgaagtc cgtgctgaca aagcggacct gcagacccag cttgtcggcg
     4261 atggcgccca gcagctggtt gtcgaagcca ctgaattgtc cggtggggtt tatgcagatg
     4321 ttcggtggag catccgagag cgtgccgacc gtcaggacgc ccggcgtgcc gaggcccaat
     4381 gcgccgacgt tgactgcact gagcggctcg accgtcgctg ttgtgtgatc gtcgtcctgg
     4441 cccatggtcg gggtttcggt cagatctttc ggcaggctga tcgcgctgtc cacgccggcc
     4501 ggagcgcatt ggttccagtc ggcagacgca ggcgcggtcg gtccggccag cgccaggcca
     4561 ctcactatca gcgttgtcgc ggccagtgcg aacagctttc ggcgcgcggc gcgccagtcc
     4621 gggcacaaat tcatcaccgc caccgtatcg accgaccggc ggttcggcgc gttaaagagt
     4681 gatcgacggc tcggcgtgcg gcgcgtcggc cccggcgaac acctgaatag ggccgggaac
     4741 aactgggccg atgtcgtcgg gcgcggcaaa cacccggcgt cgaaacagtt cggccaacat
     4801 cgtcttggcc atcaggttgg accggccgat gcatgcggcg cgtgcgccgt cggggtcacg
     4861 ccggtgtatt gcggaggtct cgtcttcgta gaacggcaac atgtcgtcgc ggctgcactg
     4921 gtaggtcatc cagaacgccc gggggatgag gtcctgcgac gcgcggatgg tggcgtgcag
     4981 ccgcggtccc gcgtactcgt cgttgatggt gcggcggtat tcgccggtga tttcggagaa
     5041 tgcccgggat tctttcgcgg agcggattga tcgcatgagc gcatcgagct gcccgaggat
     5101 ccgcggtgtg gggttggtcg ccgcacgcgc cgaggcgatc ccgttgtgca ggccgtcaag
     5161 ttcgtgatgc tcgaggacgg tggcttcgtc gaaccgctcg acgaaggcgc cccggtgata
     5221 acgggtggac acgatcccgt cgtgctcgag ttggaccagc gcctcttgaa ttgggacgcg
     5281 gctgactccc aggccaaccg cgatctcatt gcggtcgacg cggtcgccgc tgcgcagctt
     5341 cccagtcagc accagattca gaatgtggga aacgacctgg tctttttctt tgatcccgta
     5401 cttctttggc atccgctttt gagtctcttt caacccgctt cgagaagcta gcgctgagag
     5461 tttctcatgg cttggcgtga gctttcaggt gtttgacgga tgaattggga gcaaacgcaa
     5521 gtcgacggga tcagcggcta tcgcgccatc tgcacagcgc ttcggtggcg gtcaggtcgt
     5581 agtccggccc gtcgcaaccg acggtcagca gcgtgacgcc gaggccgacg agggtttcgg
     5641 cgttggcgac gaggccgccg cccccaacgg caccgttgac cgcggccgag cgttcgatct
     5701 tcgccgggtc ccggccaacg tcggcgcaat gccggccgag gacggccgac ttcgccggga
     5761 actcgtcggc ggagctgaag ctgtgccaca tatcggcgta ttcggcgacc agtcgcaggg
     5821 tcttgcgttc gcccccgccg ccgatcagga ccgggatgtc acgggtgggc tgcgggttga
     5881 gcttggccaa ccgcgccgtg atccggggca gcgcggccgc caggtcgtct aggcggctgc
     5941 cggccgtgcc gaattcgtag ccgtactcgt cgtagtcccg ttgcttccag ccggaaccga
     6001 tccccaggac gagccggccg cccgaaatgt ggtccacggt gcgggccata tcggccagca
     6061 actcgggatt gcggtaggag ttgcacgtca ccagcgcacc gatctcgatg cgtgacgtct
     6121 gctcggccca ggccccgagc atggtccaac attcgaagtg tgcgccgtcc ggatctccgt
     6181 agagcgggaa gaagtggtcc cagttgaagg cgatgtcgac accgatgtcc tcgcagcggc
     6241 gaacggcgtc gcgcagatcg cggtaacgcg gggcttgctg cggctgcaac tgcacgccga
     6301 tacgaatggg acggtctggg tgcacgggtg aggtcatgtt ttcaccgtag gcccgggggc
     6361 gtgccgggtt caccgtgcgt ccagcacccc gcgcaggatg tcgatcagcg cgcgaggctg
     6421 gtcgctttgg acggaatggc cggaattgtc gacgacgtgt gccacgcgga aattggttgc
     6481 gcgcttgccg agttcagcgg cgtcctcgtc ggtgacgaaa cccgacaagc caccgcgcac
     6541 cagggtgatg ggcgcggcta gccggtcgac gtcgtcccac aggccggcga agtcggggaa
     6601 ggtacggatg gcgtcgtagc gccagctcca attgccgttg tcaagccggc gggaattgtg
     6661 gaagacgcca cggcgcagcg ccttcacctc gcggtgcggt gccgccgcga tcgtcaggtc
     6721 cagcatcgcc tgcaagctgg ggaactcccg ctcgccgtgc atcagcgcga ccgtgccttg
     6781 ctgttcggcg gtcagctggg cgtgccgttg cagcgccgac ggggtgacat cgatcaggac
     6841 gagttcatcc accaggtcgg gcgccaccgc cgccagccgg atcgcggtca atccgcccag
     6901 cgacatgccc accacgaggt cggcggtcgg tgcgaggtcg cggaggaccg gcgccaaggc
     6961 ggcggcgttg cgctgtggtg agtagtcgcc gtcctcgcgc caggcggagt ggccgtgccc
     7021 ggggaggtcc accgccaggg ccggttcacc gagaccgacg atcaccgtgt cccaggtgtg
     7081 cgcgttttgc ccgccgccgt gcagaaaaac gatccgcggc ggacgttccg acgagggcgc
     7141 gccccaacgg agcgcactga tggtgccctc cgccgaatcc gcttgcaccc gctcgacgtc
     7201 aggtactgga cccgcgacgc cggcctgctc ggcgttctcg gacagcagcg cgaactcagt
     7261 caattccgcc agtccgtcct cagacatgtc ggtcacgtct ttagacacta cgaactccgc
     7321 cgcgacctgc gaaaggtggt ccgcgtcgct cgctcagatc gcgtcgcacc gacgcccagt
     7381 cgtcagttga gtagccgcaa cgtgtggatg tcgatgttgc ccgagtcgct gtgaggtggg
     7441 cacggcggca caccccgaac ttcttgtgac tcgtgtgagt tcatcggccg ggcgggggag
     7501 tgcccggact aagcgtcgat gatgaactct tccagttggg cacgcgcgac gtcgtcggcc
     7561 aactgcttcg gcgggctctt catcagatac gccgacgcgg caaccaccgg tccgcctatg
     7621 ccgcggtcct tggcgatctt ggccgcccgg accgcgtcga tgatgacgcc ggccgagttc
     7681 ggcgagtccc acacctcgag cttgtactcc aggttcagcg gcacgtcgcc gaacgcacgg
     7741 ccctccagcc gcacgtaggc ccacttgcgg tcgtcgagcc agcccacgtg gtcggacggt
     7801 ccgatgtgca cgtccctggt cttgaactcg cgctgcaggt tcgacgtgac cgcctgggtc
     7861 ttggagatct tcttggactc cagccgctca cgctcgagca tgttgaggaa gtccatgttg
     7921 ccgcccacgt tgagctgcat ggtgcggtcc agctggacac cgcgatcctc gaagagcttc
     7981 gccatcaccc ggtgcgtgat tgtcgcgccg acctggctct tgatgtcgtc accgacgatc
     8041 gggacgccgg cgtcggcgaa cttcttggcc cacaccgggt cgctggcgat gaacaccggc
     8101 agcgcgttga cgaatgcgac accggcgtcg atggcgcact gggcgtagaa cttgtcggcc
     8161 tcttcggacc ccaccggcag gtaggacacc agcacgtcga cgttcgcctc gcggagcgtc
     8221 tgaaccacgt cgacaggctc ggtgtcggac aactcgatgg tgtcggcgta gtacttgccg
     8281 atgccgtcga gcgtcgggcc gcgctgcaca gtcacgttgg tgggtggcac gtcggcgatc
     8341 ttgatggtgt tgttctccga cgcgaagatc gcctccgaca ggtcgaagcc gaccttcttg
     8401 gcgtccacgt cgaaggcggc cacgaacttc acgtcgcgga cgtgatactg gccgaacttc
     8461 acgtgcatca ggcctggcac gctgctgttc tcgtcggcgt cgaggtagta ctgcacgccc
     8521 tgaacaagcg aagatgcgca gtttccgacg ccgacaatgg cgactcgtac ctcagggtgc
     8581 gcctcaggcg cccccaacgg ttggtgctca ctcatagggc gttctcctaa cttcgtaact
     8641 tgctgtctca gctgttcgtt gctgcgggat tcgtcgagag gtttcatgtc tgttcggtga
     8701 ggcccggcgc cgcccgctcc gcggcgatca gttcgttgag ccacttgact tcgcgctcac
     8761 tggactcgag ccccagctga tggagctggc gggtgtagcg gtcgaaggag ctgctggccc
     8821 gggccaacgc ctcgcgcaga ccttcgcggc gttcctcgac ctgacggcga cggccttcca
     8881 ggatccgcat ccgcgcctcc gccggagtgc ggttgaagaa cgccaggtgt accccaaagc
     8941 cgtcatcggt gtagttgacc gggccggtgt cggccaccag ctcaccgaag cgacgacgac
     9001 cggcgtcggt cagttggtag acccgccggg cccgccgcac cggagtaccg gcgggggcgg
     9061 cattctcggc aatcaagcct tcgacctgca ttcggcgcag cgcgggatag agcgagccat
     9121 aggaaaacgc ccggaacgcg cccagtaggc cggtcagccg tttccgcaat tcgtagccat
     9181 gcatcggcga ttcgatcagg agtcccagaa ttgcgagctc caacatcgat ccacctcctt
     9241 tgcatgatcg gctacgtggt tcgctacttc gtggtccgct actgcgcgat ccgctacgac
     9301 ggtccgacgc ctcgcgccat cgtatcgtct cgatatattc gccacaacac taccggatgt
     9361 tggtgcgcca ttagccgccg cgtcgcgtgc gggacacgcg gctaggacgg cagattgatc
     9421 cgtttggtgg tgccgtcacc tgcgaagacg atgaaaccgc cgccgtagtc actcgagacg
     9481 tagaccgaca gcgaaagagc tcccggagta gtcgggtcag atgctcgctc gatgatcaga
     9541 taggtggttt tgacgtcgga ttgccgcatg tcaccccatc caccccggta gtcgtacgcg
     9601 agcatccggc ggtcatcgga ggggtccgcg cggtcgagaa cggcgtaggt ggggtagacc
     9661 acgagacgga atccggtggt gttcccaaat cttttgcgtg tttgcttgag ggcgttgtcg
     9721 agcagcgtgc aggtctcttg gcggtcgctg tctttggcgc gggtggtagc tgtcgccccg
     9781 gcgccgagtg gtgtcccgcc cgatttcgcc acgccgacga tcgtagaagt caggcctgga
     9841 ttcggcacgg ttgcggccga caacaggctc ggttgcgtat cggtggtagg gatcgccacg
     9901 tactctggtg tccgtgcgac tgcagcgaca agtggtggac tacgcgcttc ggcggcgctc
     9961 gctactggcc caggtgtact cgggccgcac cggtgtgtcc gaggtctgtg acgcgaaccc
    10021 atatttgctg cgcgccgcaa agtttcacgg gaaaccaagc caggtgatgt gcccgatctg
    10081 tcgcaaggag cagctcaccc tggtgtcgtg ggtgttcggc gaccatttgg gcgcggtatc
    10141 gggttcggcg cgcaccgccg aagagctggt gatgctggca acccgcttca cagagttctc
    10201 ggtccacgtg gtggaggtgt gccggacctg cagctggaat cacctcgtga agtcgtacgt
    10261 gatgggtgcg ctacgcccgc ccaagggcac cggcggcacg gggacggccc gcaacggcgc
    10321 ccggacggcc attgagtaac gaagggccct acgaccagtc gtccagcgac atgaacgagt
    10381 cggcgactga gcgcgtgggc aggcatgcga gtaacgatcg gccgggcaat cccgaacgtc
    10441 cggtccagga cggctcgggg tccggccggc gtcaccccgt tccacccgac gaccggctga
    10501 cccaaatcct gccggccgtc gtcgatggcc ggtcacccca gcggaccgat cgcatcgagg
    10561 aagtcaaggc cgcgctggac aacccttcgt cgacaccctt gcggcgggac gcgctcgacg
    10621 aggtcaaggc cgcgctcgaa agtcgcgctg caccgccgcc ctggcgaggc ggccccgttc
    10681 gcgaacaccg cccaccggaa gcacccccgc caccacccgg cccgcccggt ggacccgggg
    10741 gaccgggcgg cacattcccc ggcggccacg gaccgcgggg ccatgggggc ttggccgacc
    10801 agatcaactg gcggtgggtg cgacggctgg cctacatcgc ggcggcggtg ctggtgctgt
    10861 tgccggtcgt caccttcgcg atggcgtact tcatcgtcga cgttcccaag ccgggcaaca
    10921 tccgcaccaa ccaggtgtcg accatcctgg ccagcgacgg ctccgaaatc ggcaaaatca
    10981 ttcctcctga aggcaatcgg gtcgacgtca acctcagcca agtgccggtg cacgtgcgcc
    11041 aggcggtgat cgccgcagaa gaccgcaact tctattcgaa cccgggtttt tcgttcaccg
    11101 gcttcgcgcg cgcggtgaac aacaacctct tcggcggcgg tgatctacag ggcggctcca
    11161 cgatcaccca gcagtatgtg aagaacgcgc tggtcggatc ggcgcaacac gggttcagcg
    11221 gcttgatgcg caaggccaag gaattggtca tcgccaccaa gatgtcgggg gagtggtcca
    11281 aagacgatgt cttgcaggcc tatctgaaca tcatctattt cggccgcggc gcctacggca
    11341 tctcggccgc taccaaggcg tatttcgaca agccggtcga gcagctcacg atctccgagg
    11401 gggcgcttct ggcggcgctg attcgccggc cgtcctcgct ggacccggcg gtcgatccca
    11461 agggcgccgc ggagcgctgg aactgggtgc tcgacgggat ggtggaaacc aaggcgctgt
    11521 caccgagtga ccgcgcggca caggtgtttc cggcgaccgt cccgcccgac ctggcccgcg
    11581 cccagagcca gatcaccggc cccgacggcc tgatcgagcg tcaggtgacc aaagagctga
    11641 tggagttgtt caacatcgac gagcagaccc tgaacaccca gggtctgcag gtcaccacca
    11701 cgatcgatcc gaaggcccag caggccgcgg agaaggcggt atcgaaatac ctcgacgggc
    11761 aggatccgga catgcggtcc gccgccgtct cgatcgaccc tcacaacggt gcgatacgcg
    11821 cgtactacgg cggttccgat gccaacggat tcgacttcgc tcaggccggg ctgcagaccg
    11881 ggtcgtcgtt caaggttttc gcgctggtcg ccgcgctcga acaggggatc gggctgggct
    11941 accaggtgga cagctccccg ctgaccgttg acggcatcaa gatcaccaat gttgacggcg
    12001 agagctgcgg gacctgcaat atcgccgagg cgctcaagct ctcgctcaac accgcctatt
    12061 accggctgat gctcaaactc aagggcgggc cgcaggccgt cgcggccgcc gcgcatcagg
    12121 ccggtgtcgc cgccagcttc ccgggcgtcc cgcacacgct gtccgaggac ggcaagggcg
    12181 gacctccgaa caacgggatc gtgctgggcc agtaccagac tcggccgatc gacatggcct
    12241 cggcctatgc cacgctggcc gcatccggca tctatcaccg gccgcatctg gtgcaaaagg
    12301 tggtcaacgc cgaagggcaa gtgctgttcg acgccggcgg caaggacagc agcggcgagc
    12361 agcgcatcga caagggcgtc gcggacaacg tcacctccgc aatgcagccg atcgccgcct
    12421 attcgcgcgg ccacaacttg gccggcgggc gtccgtcggc ggccaagacc ggcacggtgc
    12481 agttgggcga cagccaggcc aacaaggatg cttggatggt cgggtacacg ccgtcgctgt
    12541 ccacggccgt gtgggtgggc acggttcaag acaacgtccc gctggtaacg gcttcgggcg
    12601 cagaggttta cggttccggc ctgccgtcgg acatctggaa gtcgaccatg gacggcgccc
    12661 tggtgggcac ttccaatgag agcttcccga agccgaccga gatcggcgga tatgcgggcg
    12721 cgcctgttgc gccgccgccc ccgcccccac cgccctcgga gaccgttatc cagcccacca
    12781 tcgaggtggc gccgggcatc accatcccgg tcggtccccc caccacgatc accgccgggc
    12841 cgccaccgcc cggcggcccc caacccggcg gtgtcccgcc gggcggcccg caagctccgc
    12901 caccaccccc gtgaccggcg cgcaacagca gagcgagacc atctcgccgc tgccgttggc
    12961 cgccgatcgg cgtagcgccg acaaccgtga ttgccccagc cgcaccgact ttttgggcgc
    13021 ggccctggcg gacgtcgtgg gaggaccggt cggccggcac gcgctgatcg gccgcactcg
    13081 ggtgatgacc ccggtacggg tgatgtttgt gatggcgctg gtgttcctgg cgctcggttg
    13141 gtcgacgaag gcggcctgct tgcagagcag tgggacgggg acgggtgacc agcgcgtcgc
    13201 caactgggac aaccaacgcg cttactacga gttgtgctac tccgatacgg tgccgctcta
    13261 cggcgcggag ttgttgaatc agggcaagtt tccgtacaag tccagttgga tcgagagcga
    13321 cgccaacggc gcaccgcaga cccgctacga cggccagatc gcggtgcggt acatggagta
    13381 tccggtgctg accggcatct atcagtacgc gtcgatggcg ctggccaaga cctacacggc
    13441 ggtgagcaag gttgcgcccc ttcccgtggt cgccgaggtg gtgatcttct tcgacgtcgc
    13501 ggcgttcggg ttgtcgttgg cgtggctggc gacggtgtgg gcatccgcgg gactgtcggg
    13561 ccgccgcgtc tgggatgcgg cactggtggg cgcgtcgccg ttggtgatct ttcagatatt
    13621 caccaacttc gacgcgctgg caacggcatt cgcgatgggc gggctgttgg cgtgggcgcg
    13681 acgaaaaccg gtgctcgccg gtgtgctgat cggattgggc gccgcggcca agctgtaccc
    13741 gttgttgttc ctgggcccga tgttggtgct gggtatccgg actgggcgcc tcggtgcgct
    13801 gtggcgcacc gcagccgcgg ccgtgacgac ctggttgttg gtgaatcttc ctgtcttggt
    13861 gctctttccg cggggctggt cggagttttt ccggctcaac acccgtcgcg gtgatgacat
    13921 ggactcgctg tacaacgtcg tgaagtcatt caccggctgg cgcggcttcg atcccaacct
    13981 gggcttctgg gagccgccca cggtgttgaa cacggtggtc acggtgacgt tcgcggcgtg
    14041 ttgtgcggcc atcgcttacg tggtgctcac cgccccgcga cgcccacgag tggcgcagct
    14101 ggcgttcttg gcggtcgcgg ccttcctgtt gaccaacaag gtgtggagcc cgcagttttc
    14161 gttgtggctg gtgccgctgg ccgtactggc gttgccgcat cgtcgaatac tgttgacgtg
    14221 gatgacgatt gacgcgctgg tgtgggtgcc gcggatgtat tacctgtacg gcaatccgaa
    14281 ccgtggactg cccgagcaat tcttcaccac gacggtgttg ctgcgtgacg tcgcggtcat
    14341 ggtgctgtgc gcgttggtga ttcgggagat ttaccggcct gaggaggacc tggtgcgttg
    14401 gggtggacga gtcgacgatc cggggggcgg tccgttcgac cgcgcccccg atgccccgcc
    14461 cgactggctg cccgactggc tgcgtccgcc ggcgctgcgg cgggcggttg cggaggcgtc
    14521 agaaaccgac gcggaaactg aagcagtggc cggcgccagg cacgcgtgat gttggtcgcg
    14581 ataccgctgt tcccgcgttt caccgcactc gacgcggttg gaccctatga agtgctgcaa
    14641 cgcattccat cgatcgacgt ggtattcgtc gggcaccggc gtggcgaggt gcgcacggaa
    14701 aacgggatgc tcggtatcac ttgcgacgcc acattcggcg aagtcagcgc acccgatgtg
    14761 gtggtcgtgc ccggtggtat cgggacccga cagctgctgc acgacgaggt catccgcgac
    14821 tggtttcgat cggtacatcc gcacaccagg ttcacgacct cggtgtgtac cggcgcgctg
    14881 ttgcttgcgg ccgcgggcct gctcgatggc ttgaccgcaa cgactcactg gcgggcagca
    14941 agcctgctca acgaattggg cgcgcgttat gtgcccgacc gagtcgtcga gcacctcccg
    15001 cagcgactca tcaccgccgc gggcgtgtcg agtggccttg acatggcgct gcggctggcc
    15061 gaactccttg tcgaccgact ggccgcccag gccgcccagt tgctcatcga atacgacccc
    15121 cagccaccgt tcgacac
//
LOCUS       DASSGQ010000183         9509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_863960,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000183 DASSGQ010000000
VERSION     DASSGQ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9509
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863960"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..964
                     /locus_tag="VFI55_08070"
     CDS             101..964
                     /locus_tag="VFI55_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET7074020.1"
                     /translation="MTDPPDGAVEIGLTGRPPRPVPEPEPRTSHGPAKVVAMCNQKGG
                     VGKTTSTINLGAALAEYGRRVLLVDIDPQGALSAGLGVPHYELEKTIYNVLVEPRVSI
                     DDVMLSTRVKHLDLVPSNIDLSAAEIQLVNEVGREQTLARALHPVLDRYDYVLIDCQP
                     SLGLLTVNGLACADGVVIPTECEFFSLRGLALLTDTVDKVRDRLNPKLEISGILITRY
                     DPRTVNSREVMARVVQRFGDLVFDTVISRTVRFPETSVAGEPITTWAPKSTGAIAYRA
                     LAREFIHRFGA"
     gene            952..1794
                     /locus_tag="VFI55_08075"
     CDS             952..1794
                     /locus_tag="VFI55_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET7074021.1"
                     /translation="MRRVKGSVNGETPPTNGFQVRLTNFEGPFDLLLQLIFAHRLDVT
                     EVALHQVTDDFISYTREIGPQLGLEETTAFLVIAATLLDLKAARLLPTGQVEDDEDLA
                     LLEVRDLLFARLLQYRAFKHVAELFAELEAAALRSYPRAVSLEERFTDLLPEVMLGVD
                     AERFAEIAAIAFTPRPVPTVATEHLHDLKVSVPEQAEKLLAILAARGSGEWATFGELV
                     ADCRASMEVVGRFLALLDLYRTRTVAFDQSEPLGVLQISWTGERPTSEALIVVPDESV
                     PDES"
     gene            1868..2563
                     /gene="scpB"
                     /locus_tag="VFI55_08080"
     CDS             1868..2563
                     /gene="scpB"
                     /locus_tag="VFI55_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET7074022.1"
                     /translation="MTEELPGIDLGYGIPDIAEAAPLDADELGRVLEALLLVVDTPVT
                     AEALAAVTQQPVYRIAAKLQQMADELTERDSGIDLRKTSGGWRMYTRARLAPYVEKLL
                     LDGARSKLTRAALETLAVVAYRQPVTRARVSAVRGVNVDAVIRTLLARGLITEAGVDD
                     DTGAVTFATTEMFLERLGLTSLADLPDIAPLLPDVDTIEDLSESLDSEPRFIKLSGRP
                     AAEQELSFDVDRD"
     gene            2563..3318
                     /locus_tag="VFI55_08085"
     CDS             2563..3318
                     /locus_tag="VFI55_08085"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET7074023.1"
                     /translation="MVEADADTSGVRLQKVLSQAGIASRRAAEKLIIDGRVEVDGQVV
                     TELGTRVDPDVSVIRVDGARVVVDESLVYLALNKPRGMHSTMSDDRGRPCIGDLIERR
                     VRGTKNLFHVGRLDADTEGLILLTNDGELAHRLMHPSHEVPKTYLATVTGSVPRGLGK
                     KLRAGIELDDGVTKVDDFAVVDAIPGKTLVRLTLHEGRNRIVRRLLAAVGYPVEALVR
                     TDIGAVSLGNQRPGSIRALRFDEIGQLYKAVGL"
     gene            3315..3998
                     /gene="cmk"
                     /locus_tag="VFI55_08090"
     CDS             3315..3998
                     /gene="cmk"
                     /locus_tag="VFI55_08090"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET7074024.1"
                     /translation="MTSVVVAIDGPAGTGKSSVSRGLAQALGARYLDTGAMYRMVTLA
                     VLRAGVDPADAGNVAAIASAVHMSVDYNPDGDRYYLAGEDVSAVIRGDEVTRAVSAVS
                     AVPAVRSRLVGLQREMANGPGNVVVEGRDIGTVVLPDAPVKIFLTASAETRARRRNDQ
                     NVAAGLPDDYEGVLADVRRRDHLDSTRAVSPLRAAADAVIVDTSDMTQHEVIAHLLAL
                     VKERSGAVR"
     gene            3995..5398
                     /gene="der"
                     /locus_tag="VFI55_08095"
     CDS             3995..5398
                     /gene="der"
                     /locus_tag="VFI55_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET7074025.1"
                     /translation="MTQDGTWTDESDWEFGDSDAMDAEEAGPAPVVAIVGRPNVGKST
                     LVNRILGRREAVVQDVPGVTRDRVSYEALWTGRRFVVQDTGGWEPDAKGLQQLVAEQA
                     SVAMRTADAVILVVDATVGATTADEAAARILLRSGKPIFLAANKVDSDKVEADAAALW
                     SLGLGEPHAISAMHGRGVADLLDEVLAALPEVSEAAPAAGGPRRVALVGKPNVGKSSL
                     LNKLAGDQRSVVHDVAGTTVDPVDSLIELGGKVWRFVDTAGLRRKVGQASGHEFYASV
                     RTHSAIDAAEVVVVLIDASVALTEQDQRVLSMVIEAGRALVLAFNKWDLVDEDRRDLL
                     ERDIDRELAQLRWAQRVNISAKTGRAVQKLVPAMETALASWDSRVATGPLNTWIKEVV
                     AATPPPVRGGKQPRILFATQAAARPPTFVLFTSGFLEAGYRRFLERRLRESFGFEGSP
                     IRINVRVREKRGPARRR"
     gene            5501..7153
                     /locus_tag="VFI55_08100"
     CDS             5501..7153
                     /locus_tag="VFI55_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PPE domain-containing protein"
                     /protein_id="HET7074026.1"
                     /translation="MTYVTIQPQMLAAAAADAAGIGSEIEEASAAAAGRTTGVVAAAG
                     DEVSAATATLFNECAQEFQAILGQAEAFHAQFARALGAASSAYEEAEAVNAASVAPLR
                     PSAANVTLVMGGSGNPIPPQSYVNAVVTKYITPNFPAFTVSNAQSLFTPEALYPLTGI
                     KDLVPNVSVSQGVTILNNAIQQQLAAGNNVAVVGFSQSSIIASLEMQMLDPSGTPSSL
                     PVVFSLLGDPMNPNGGLLARFPGLTMPSLGFTFYGATPGDDFVTKIYTIEYDGFADFP
                     QYPIDFLADLNAIAGIYYVHGTYADLTPTQINSAVPLTNTVGPTTTSYYMIPNPHLPL
                     LEPLRAVPVVGTPLADLLEPDMRVLVNLGYGSPSQGWSTGPPNVPTPFGLFPPVNPGE
                     VAGDLVIGTQQGIGAFVSDLSAEGSGVSPSGLLQALPSMPGSSPIAPPPASIPSFIEG
                     LQAANTSFTNAFSSALSTGYSVLLPTADFATAALTSIPSYDVNLFLNGILQAVNGDPV
                     GLINAVGDPIAADTALFTIAGGFEAIVLASAAESIVEDFTSL"
     gene            7232..7305
                     /locus_tag="VFI55_08105"
     tRNA            7232..7305
                     /locus_tag="VFI55_08105"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7266..7268,aa:Pro,seq:ggg)
     gene            7390..8589
                     /locus_tag="VFI55_08110"
     CDS             7390..8589
                     /locus_tag="VFI55_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET7074027.1"
                     /translation="MGKRRANSEGNVYQRPNGRWEARLRYTDPTTGQPKRVSVYADTQ
                     KGALAELDKVRKRITAGQPPRDATTTVGAWMAHWRDVTLAASSRKPATQELYGALSKK
                     HVEQSVIGQVRLDRLRPSHVEKLIIDMRSQTKPGKVTPECPHPAAVRALSDSTIRSTY
                     VVLRAGLDAAVRDGLLGVNPATLVPRPGVARREAVHLEQVDLARLLDAARESSRYYPA
                     IVLAATTGMRRGEIAALKWRDIDLAAGVLRVSATTSRIAGQLVVSAPKTERSRRAVPV
                     PAAVVDLLRAQRVSLKKDRLRAGDQWREHGLVFPTEFGTPVDPRSLLRVVQAAARRAG
                     LPETVGVHTLRHSAATGWLEAGVHIRAVGDLLGHSSVAVTGDVYGHVTDSAGRGAVEG
                     LAGRLGL"
     gene            8784..9011
                     /locus_tag="VFI55_08115"
     CDS             8784..9011
                     /locus_tag="VFI55_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7074028.1"
                     /translation="MPNTDTTPQRLLPIGRDKNHGTRAVLGNISDVTIYKMIKRGDLD
                     AVKIGRLTFITQASIDAFVARGIAKQELLKP"
     gene            9076..9318
                     /locus_tag="VFI55_08120"
     CDS             9076..9318
                     /locus_tag="VFI55_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074029.1"
                     /translation="MQQNNEDPRRGTTPAEGLQNQPDTSIVDDRVSNPPASKWHGDFR
                     RRVQRAAIDEHYFDMCPGYVQQAALQLLGRREGDAA"
     gene            9315..>9509
                     /locus_tag="VFI55_08125"
     CDS             9315..>9509
                     /locus_tag="VFI55_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074030.1"
                     /translation="MSAAPKWADDASCFFCIPGRPCREHAVIPFRQRLQDAGIIKDKP
                     PHNPAAPPAGTPNQAYAVATL"
BASE COUNT         1622 a         3078 c         3214 g         1595 t
ORIGIN      
        1 gcccgtattg gctggttaat tgcctgcggg tcgtccggcg tacgcgcgcg aacccgttag
       61 actttcctgc gatgttcacc tcccgaacac cgcgggcatg atgaccgacc ccccggacgg
      121 tgctgtcgag atcggcctga cgggacgccc gccgcggccg gtcccggagc ctgagccgcg
      181 cacctcgcac ggccccgcca aggtcgtcgc gatgtgtaac cagaagggcg gcgtcgggaa
      241 aaccacgtcg acgatcaacc tgggcgccgc gctggccgaa tacggcaggc gggtgttgct
      301 ggtggacatc gacccgcagg gcgcgctgtc cgcgggcctg ggtgtcccgc actacgagct
      361 ggaaaagacc atctacaacg tgctggtcga gccgcgagtg tcgatcgacg acgtgatgct
      421 ttccacccgg gtcaagcact tggatctggt gcccagcaat atcgacctct cggccgcgga
      481 gatccagctg gtcaacgagg tcggccgcga gcagactctg gcccgggcgc tgcacccggt
      541 gctggaccgg tacgactatg tgctgatcga ctgccaaccg tcgctgggcc tgctcaccgt
      601 caacgggctg gcgtgcgcgg acggcgtggt gatccccacc gagtgcgagt tcttctcgct
      661 gcgcggactg gcgctgctca ccgataccgt cgacaaagtg cgcgaccggc tcaatccaaa
      721 gctggagatc agcggcatcc tgatcacgcg ttacgacccg cgtaccgtca actcgcgcga
      781 ggtgatggcc cgggtggtgc agcggttcgg tgacttggtg ttcgacaccg tgatcagccg
      841 gaccgtccgc ttcccggaga ccagcgtggc aggcgagccc atcaccacct gggccccgaa
      901 atcgactggc gctattgcct accgcgcgtt ggcccgtgag ttcatccacc gattcggcgc
      961 gtgaagggca gtgtgaacgg tgagacgcca cctacgaacg gcttccaggt ccgactgacc
     1021 aacttcgagg ggccgttcga tctgctgctg cagctgatct tcgcccatcg tctcgacgtc
     1081 accgaagtgg cgctgcatca ggtcaccgac gacttcatct cgtacacccg ggagatcggt
     1141 ccccagctcg gcttagagga gaccaccgcg ttcctggtga tagccgcgac cctgctcgac
     1201 ctgaaagcgg cccgcctgct gccgaccgga caggtcgagg acgacgagga cttggcgcta
     1261 ctcgaggtgc gcgacctgct attcgcccga ctgctgcaat accgcgcgtt caagcacgtc
     1321 gcggagctgt tcgccgagct cgaggccgcc gcgctgcgca gctatccgcg cgccgtgtcg
     1381 ctcgaggaac ggttcaccga cctgctgccc gaggtgatgc tcggggtcga cgccgaacgg
     1441 ttcgccgaga tcgccgccat cgcgttcacc ccgcggccgg tcccgacggt ggccaccgaa
     1501 catctgcacg acctcaaggt ctcggtcccc gagcaggccg aaaaactgct ggcgatcctg
     1561 gcagcgcggg gcagcggcga gtgggccacg tttggcgagc tggtcgccga ctgtcgggca
     1621 tcgatggagg ttgtcgggcg cttcctggcg ctgctcgatc tgtatcggac ccgcacggta
     1681 gcattcgacc agtcggagcc gcttggcgtg ctccagattt cgtggaccgg ggagcgtccg
     1741 accagcgaag cgttgatagt agtgcccgac gaatcggtgc cggacgaatc atgactcgcg
     1801 ccgagacgat gcattgcgca gtgatgttgg gggcaccgcc cgcttgcggg ggagaggaac
     1861 ggcgctcgtg actgaagagt tgcccggcat cgacctgggc tatggcatcc cggacatcgc
     1921 tgaggcggcc ccgctggacg ccgacgaact gggccgcgtg ctggaggcgc tgttgctggt
     1981 ggtggacact ccggtcaccg ccgaggcgct ggccgcggtc acgcagcaac ccgtctaccg
     2041 gatcgccgcg aagctgcagc agatggccga cgagctcacc gaacgcgaca gtggcatcga
     2101 cctgcgaaag accagcgggg gatggcggat gtacacgcgc gcccgcttgg cgccgtacgt
     2161 cgagaaactg ctgctagacg gtgcccgatc caagctgacc cgcgcagcgc tggagacgct
     2221 ggccgtggtg gcctaccgtc agcccgtgac acgtgcccga gtgagtgctg ttcgcggcgt
     2281 caacgtcgac gcggtcatcc gcaccctgtt ggcgcgcggc ctgatcaccg aagccggcgt
     2341 cgacgacgac accggggctg tgacgttcgc caccacggag atgttcctgg aacgcttggg
     2401 gctgacgtcc ctggccgacc tccccgatat cgcgccgttg cttcccgatg tcgacacgat
     2461 cgaggacctg agcgaatccc tggacagcga gccacgtttc atcaagctgt cggggcgccc
     2521 ggcggccgag caggagctgt cgttcgacgt ggatcgggat tgatggtcga ggctgacgcg
     2581 gatacttcgg gagtccgtct acaaaaggtg ttgtcccagg ccggaattgc gtcgcgccgc
     2641 gccgccgaga agttgatcat cgacggtcgc gtcgaagtgg acggtcaggt ggtgaccgag
     2701 ttgggtacgc gcgtcgaccc cgacgtctcg gtgattcgcg tcgacggggc ccgggtcgtc
     2761 gtcgacgagt cgctggtgta tctggccctg aacaagccgc gtggtatgca ttcgacaatg
     2821 tcggacgacc ggggccggcc gtgtatcggc gacttgatcg agcgccgggt gcgcggcacc
     2881 aagaatctgt tccacgtcgg gcggttggac gccgacaccg aggggctgat cctgctcacc
     2941 aacgacggcg agctggcaca ccggttgatg catccctcgc acgaggtgcc caagacgtat
     3001 ctggcgacgg tgaccgggtc ggttccgcgc gggctgggca agaaattgcg ggctggaatc
     3061 gagctggacg acggcgtgac caaggtcgac gatttcgcgg tggtcgacgc cattccaggt
     3121 aagacgttgg tgcggttgac attacatgag ggacgcaacc gcatcgtgcg ccggctgctc
     3181 gcagccgtcg gctacccggt ggaggcgttg gtgcgcactg acatcggtgc ggtgtcgctg
     3241 ggcaatcagc gcccggggag catccgcgcg ctgcgctttg acgagatcgg gcaactgtac
     3301 aaagcggtgg gcctgtgacc tcagtcgtgg tggccatcga cggtccggcg ggaaccggaa
     3361 agtcgtccgt atcaagggga ttagcgcaag cactgggggc gcgctatctg gataccgggg
     3421 ccatgtaccg gatggtgacg ctggcggtgt tgcgcgccgg ggtcgatccg gcggatgccg
     3481 gcaacgtcgc ggcgatcgcg tcggccgtgc acatgtcggt ggactacaac cccgacggag
     3541 atcgttacta ccttgccggc gaagatgttt cggcggtaat ccggggcgac gaggtcactc
     3601 gggcggtttc ggcggtatcc gcggtccccg cggtgcgcag ccggctcgtc ggactgcagc
     3661 gcgaaatggc gaacggcccg ggcaatgtcg tcgtcgaggg ccgcgacatc gggaccgtcg
     3721 tattgcccga cgcgccggtg aagatcttct tgaccgcatc cgccgaaacc cgcgcccggc
     3781 gccgcaacga ccagaacgtc gcggcgggcc tgcccgacga ctatgagggc gtgctggccg
     3841 atgtgcgccg tcgcgatcat ctcgactcca ccagggcggt gtcgccgctg cgcgcggcgg
     3901 cggacgcggt gatcgtcgac accagtgaca tgacgcaaca cgaggtgatc gcccacctgc
     3961 tggcgttggt caaggagcga agcggggcgg tgcggtgacc caggacggca cttggaccga
     4021 cgaaagcgat tgggaattcg gtgattccga cgcgatggat gccgaggaag cgggtcccgc
     4081 gccggtggtg gctatcgtgg gccggcccaa cgtcggcaag tcgactctgg tcaaccggat
     4141 cctggggcga cgcgaagcgg tggtgcagga tgttcccggg gtgacccggg accgggtgtc
     4201 ctatgaggcg ctgtggaccg gacgccggtt cgtcgtgcag gacaccgggg gatgggagcc
     4261 cgacgccaag ggcctgcagc agttggtggc cgaacaggcg tcggtggcca tgcgcactgc
     4321 cgacgcggtg atcctggtgg ttgacgccac cgtcggcgcc accacggccg acgaggccgc
     4381 cgcccgtatt ctgctccggt ctggcaagcc aatcttcttg gccgccaaca aggttgacag
     4441 cgataaggta gaagcggacg ccgcggcgct gtggtcgctg ggcctcggcg agccgcacgc
     4501 gatcagcgcg atgcacggtc gcggtgtggc cgatctgctc gacgaggtgc tggctgcgct
     4561 gcccgaggtg tcggaagcgg cgccggccgc gggcggtcct cggcgggtgg cgctggtcgg
     4621 caagccgaat gtgggcaaga gttcattgct gaacaagctt gcgggcgacc agcgttcggt
     4681 ggtgcacgac gtggccggaa cgaccgtgga tccggtggac tcgctcatcg agttgggcgg
     4741 caaggtgtgg cggttcgtcg atacggcggg cttgcggcgc aaggtcggtc aggccagcgg
     4801 gcacgagttc tatgcctcgg tgcgcaccca ttcggccatc gacgcggccg aggtggtggt
     4861 cgtgctgatc gacgcgtcgg tggcgctgac cgagcaggac cagcgggtgc tgtcgatggt
     4921 gatcgaggcc ggacgcgccc tggtgctggc gttcaacaag tgggacttgg tcgacgagga
     4981 ccgacgcgac cttctggagc gcgacatcga ccgtgaactg gcacaactgc gctgggcgca
     5041 gcgggtcaac atctccgcca agacgggccg cgcggtgcag aaactggtgc cggccatgga
     5101 gactgcgctc gcctcgtggg actcccgggt cgctaccggc ccgctgaaca cgtggatcaa
     5161 ggaggtggtg gcggccacgc cgcctccggt gcgcgggggc aagcagccac gcatcctgtt
     5221 cgccacccag gccgccgccc ggccgcccac gttcgtgctg ttcaccagcg gttttctcga
     5281 ggcgggttac cggcgtttcc tggaacgacg gctgcgtgag tcgttcgggt tcgagggcag
     5341 tccgatccgg atcaacgtgc gggtgcgcga aaagcgggga ccggccaggc gccgctgagc
     5401 tcgaccggcc ggatcgagtg tgcgcccggg ccaggcaaac gtcagacagg ctggccagat
     5461 cgctctacgc tgtcagtgcc gcagcgtgaa ggatggcccg atgacgtacg tgactatcca
     5521 gccgcagatg ctggcggcgg ccgccgcgga tgcggccggg attggttcgg agatcgaaga
     5581 ggccagtgcc gccgccgcgg gccggacaac gggtgtggtg gcggcggccg gcgatgaggt
     5641 atcagcggcc actgcgacgc tgttcaacga gtgtgcccag gagtttcagg cgatccttgg
     5701 gcaggcggaa gcgtttcacg ctcaattcgc gcgggccttg ggggcagcct cgagcgccta
     5761 cgaagaggca gaggccgtga acgccgcctc cgtggcgccg ctgcggccat ccgcggcgaa
     5821 cgtgactttg gtcatgggcg gcagtggcaa cccgataccg ccgcaaagct acgtgaatgc
     5881 cgttgtcacg aaatacatca cgccgaattt cccggctttc accgtgagca acgcgcagtc
     5941 cctgttcact ccggaggcgc tctatccgct gacagggatc aaggatttgg tgccgaacgt
     6001 gtcggtgtcc cagggcgtta cgatcttgaa caacgcgatc caacagcaac ttgccgccgg
     6061 gaacaacgtt gccgttgtgg gcttctcgca gagctcgatc atcgcctccc tggaaatgca
     6121 gatgctcgat cccagcggca cgccgagctc gctccccgtc gtcttctcac tgctcggtga
     6181 tcccatgaat cccaacggcg gcctcctggc gcggttcccc ggcctgacca tgccgagtct
     6241 aggtttcacg ttctacggcg ccactccggg cgacgacttc gtgacgaaga tctacacgat
     6301 cgaatacgac ggcttcgccg acttcccgca atacccgatt gatttcctgg ccgacctcaa
     6361 cgccattgcg gggatctatt atgtgcacgg cacctacgcg gatctgactc ccacacagat
     6421 taattcggct gttccgctga cgaacaccgt gggccccacc acgaccagtt actacatgat
     6481 tcccaacccg cacctgccac tgctggagcc gctgcgtgcg gtcccggtcg tgggaactcc
     6541 gttggcggac ctgctcgaac cggacatgcg ggtgctggtc aacctcggct acggcagccc
     6601 cagccagggc tggtcgaccg ggccgccgaa tgtgcccaca ccattcggtc tcttcccgcc
     6661 ggtcaacccc ggcgaggtcg ccggcgatct ggttatcggc acccagcaag gaatcggtgc
     6721 cttcgtgagc gatctgagcg ccgagggttc cggcgtgtcg ccgtctggct tgttgcaggc
     6781 gctgccatcg atgccggggt cgtcgcccat agcgccgccc ccggcgtcaa tcccgagctt
     6841 catcgagggc ctgcaggcgg cgaacaccag tttcaccaat gccttctcga gcgcgctctc
     6901 gaccggctac tcggtgctgc tacccaccgc ggacttcgcg accgccgccc tgacctctat
     6961 cccgtcctat gacgtcaatc tgttcctaaa cgggatcttg caagcggtca acggcgaccc
     7021 agtggggctc atcaatgcgg tgggggaccc gatcgctgct gataccgcgc tgttcaccat
     7081 tgcgggcggt ttcgaagcca tcgtcctcgc gtctgcggcc gaatcgatcg tcgaggattt
     7141 caccagtctc tgacatgttc cgaggggatg gcggcctagc gcttcgccta cggtaggctt
     7201 tcgacctgcc gccggtggag ccaccggcga ccgggctgtg gcgcagtttg gtagcgcact
     7261 tgactggggg tcaagtggtc gcaggttcaa atcctgtcag cccgactagc acatacacgg
     7321 ttctttggcc gccgaggacc ggcccatacg cccattccta cgcccaccag ctcgctacac
     7381 ttagcagcaa tgggcaaacg gcgcgccaac agcgaaggca acgtctacca gcggccgaac
     7441 ggccgctggg aagcccggct gcgctacacc gatccgacca ccggccaacc caaacgtgtc
     7501 agcgtgtacg cggacaccca gaagggtgcc ctggccgagc tggacaaggt acgcaaacgc
     7561 atcacggccg gccagccgcc acgcgacgcc accacaaccg tcggcgcatg gatggcgcac
     7621 tggcgggacg tgacgctggc cgcgtcatcc cgcaaaccgg ccacccagga gctgtacggg
     7681 gcgctttcca agaagcatgt ggagcagtcg gtcatcgggc aggtacggct cgaccggcta
     7741 cgcccgtccc acgtcgagaa gctgatcatc gacatgcgat cccagacgaa accgggcaag
     7801 gtgaccccgg aatgtccgca cccggccgcg gtgcgcgcgc tctccgactc aacgatccgg
     7861 tccacctatg tggtgttgcg ggccggcctg gacgccgcgg tccgtgacgg gctgctcggc
     7921 gtcaaccctg caaccttggt gccgcggcct ggcgtagccc gccgcgaagc tgttcacctt
     7981 gaacaggtcg acctggcgcg actgctggac gctgcccgcg agtcctcccg ctactatccg
     8041 gcgatcgtgc tggccgcgac gaccggcatg cgacgcggag agatcgccgc cctgaagtgg
     8101 cgcgacatcg atctggctgc cggtgtgctg agggtgtcgg ccacgacatc gcggatagcc
     8161 ggtcagctgg tcgtgtccgc gccgaagacg gagcggtccc ggcgggcggt acccgtccca
     8221 gccgccgtcg tggatttgct gcgcgcacaa cgggtctcgt tgaagaagga caggttgcgg
     8281 gcgggtgacc agtggcgcga gcacggccta gtgttcccga ccgaattcgg cacaccagtg
     8341 gatccgcgga gcctgctgcg ggttgtgcag gccgccgcgc ggcgggctgg gctgccggaa
     8401 accgttgggg tgcacacgct tcggcattcg gcggcgacgg gctggctgga ggctggtgtg
     8461 catatccggg cggtgggtga cttgctgggg cactcctcgg ttgcggtgac gggcgacgtg
     8521 tacgggcatg tgactgatag cgcggggcgt ggcgcggtgg aggggttggc tgggcggctc
     8581 ggcctgtagt cgaagctgtt accgaatcgt taccgtgcgt gggttatgcg gttctgtgcg
     8641 cgcatgtgcg tgtctgtgcg catggcttgg tgctcgaagg gcgctccgcc gcaacgcaag
     8701 gcggcgatta tggcgtaccc caatggtaat gctagaaggg cacttgccta tcactggcac
     8761 aaaacgtgaa cggaacccaa cccatgccca acactgacac cacgccgcag cggctgctcc
     8821 ccatcggccg cgacaaaaac cacggcacgc gcgccgtcct cggcaacatc agcgacgtca
     8881 ccatctacaa aatgatcaag cgcggcgacc tcgatgccgt caagatcggc cgcctcacat
     8941 tcatcaccca agcatccata gacgcattcg tcgcgcgcgg catagccaag caggaactac
     9001 tcaaaccata acctgcacaa cacctttcaa aaacggattc tcggctgcga accaacccga
     9061 ttgaagggca ccacaatgca gcaaaacaac gaagaccctc ggcgaggcac cactcccgcc
     9121 gagggtctcc agaaccaacc cgatacatcc attgtagacg atcgcgtttc taacccgcca
     9181 gccagcaagt ggcacggcga cttccgtcgc cgtgtgcaac gcgccgcgat cgacgaacac
     9241 tacttcgaca tgtgccccgg ctacgtccag caagcggcgc tgcagctcct cggccgccga
     9301 gaaggtgatg cagcatgagc gccgcaccga aatgggcaga cgacgccagc tgcttctttt
     9361 gcataccggg ccgcccgtgc cgcgaacacg ccgtcatccc gttcaggcaa cgcttacaag
     9421 acgctgggat catcaaagac aagccgccac acaaccccgc ggcgccgccg gccggaactc
     9481 ccaatcaggc ctacgctgtc gccaccctc
//
LOCUS       DASSGQ010000184        13726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_924965,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000184 DASSGQ010000000
VERSION     DASSGQ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13726
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924965"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..1193
                     /gene="hypE"
                     /locus_tag="VFI55_08130"
     CDS             81..1193
                     /gene="hypE"
                     /locus_tag="VFI55_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HET7074031.1"
                     /translation="MSKSAGEYLSSGPRFAEGEVIERIESFRRRRPRLLDDHVTLAHG
                     AGGKASAALVDAVFLEAFRNPLLESLGDSAVLTLPSGERLAMSTDSFVVQPRRFPGGS
                     IGELAVHGTCNDLAMAGAVPSWISAAFVLEEGFPIAELKEIVADMAAAAANAGVHVVT
                     GDTKVVAKGAADGLFITTTGAGIIPAGRTLSPASVRAGDKVLLSGSMGDHGMAVMLAR
                     GDLAIEADIASDTASLSPLVELLMAAAPSTRWLRDATRGGVGTVCNELAQASGLGVLL
                     DEERLPVRPMVNGACELLGIDPLYVANEGKFVAVVAPEEADAGLAALRSHPLGAEAAE
                     VGEILTEPAESVVLRTGFGGTRIVDMLVGDPLPRIC"
     gene            1220..1492
                     /locus_tag="VFI55_08135"
     CDS             1220..1492
                     /locus_tag="VFI55_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HET7074032.1"
                     /translation="MCLGIPGRIVEITDPANYLAKVDVSGVQRTISVRLLEDDMPEPD
                     EWVLVHVGFAMAKIDETEALLTLAAVKKLGEAYTTEMEAFDSSAIV"
     gene            1505..2641
                     /gene="hypD"
                     /locus_tag="VFI55_08140"
     CDS             1505..2641
                     /gene="hypD"
                     /locus_tag="VFI55_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HET7074033.1"
                     /translation="MKFVDEFRDPAAARKLLGAIEHLAGERDHFKFMEVCGGHTHTIY
                     RHGIEHLLPDNVELVHGPGCPVCVIPMGRVDDAMWLAGQPDVIFTCFGDMMRVPGSSG
                     SLLDAKARGADVRFVYSPLDALKIATDNPGKQVVFFAIGFETTAPSTAVTLVRARDLG
                     LTNFSVFCNHVTIVPPIKAILESPDLRLSGFIGPGHVSTVVGNRPYRFVPEVYRKPLV
                     VAGFEPLDILAAVAMLLRQIREGRCEVENQYKRVVPEHGNPAALALMGKVFALRPHFE
                     WRGLGFISQSALRLHDDFAEFDAELRFSMPGVRVADPKACQCGEVLKGVLKPWECKVF
                     GTACTPETPIGTCMVSPEGACAAYYNFGRMHRDAVKLVGQSGRV"
     gene            2646..3386
                     /locus_tag="VFI55_08145"
     CDS             2646..3386
                     /locus_tag="VFI55_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019928927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6390 family protein"
                     /protein_id="HET7074034.1"
                     /translation="MTDARGAEMFARYAYAPNALGYCGPPLGATLRDGSVDDVRKAAT
                     KFSGAWPYLRVLSALTGIADPLDYRLVESYWLGGGVGADVDPEDFFDRLLAIIGPQAG
                     RYWSHLTADLAREAAGNHCFHVFGVYPWTRFLGRGEHPLNVLDNCRITWGTVVSRVDD
                     NVEVMCQRLVWDGQALTLSEPSLRLLDVWADGYSAVPEVGVADEVAVHWGRLCGRLSR
                     EQVRALRDSTDRQLRVTSQRLARALLPD"
     gene            3392..4555
                     /locus_tag="VFI55_08150"
     CDS             3392..4555
                     /locus_tag="VFI55_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase/phosphodiesterase
                     family protein"
                     /protein_id="HET7074035.1"
                     /translation="MPGSICDVLPAAAALLGAPGAVDTLGLTDWVDGRVERVVVMLVD
                     GMGWHLLPKLAPSAPLLASVLCGGIGRLSELSCTFPSTTPSSLVSLGTGARPGAHGIL
                     GFALNIPGTDRVLNHIRWRDDPPYAEWQPLPTWFERLEQAGVSARAVLPGAFIGSGLT
                     DAAYRGARFRPTDATDDYARTLADEVNAGPGLIYGYTTELDTAAHVFGIGSPSWHTAA
                     AHVDELLRRLLETLPAKAVLLVTADHGGLNVPPDARADLDTDPRLAAGVRVVAGEPRV
                     RYLHTEPRAAPDVQATWTEVLNGRVQVYLREEAVATGMFGPVNPSHLARIGDVVVVCT
                     GDSAVLATAHEPPETARLIGFHGAATAAEMAIPLIAFGSAQVDDDPPVMHTGL"
     gene            5237..6211
                     /locus_tag="VFI55_08155"
     CDS             5237..6211
                     /locus_tag="VFI55_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPE family protein"
                     /protein_id="HET7074036.1"
                     /translation="MNFATIPPEIHSGRMHAGPGSGSLTEAVTAWERLAVRLSTAAAD
                     YRAVTSKLAAWGGPASTALTEAAAPYIVWLAATAAHAEHAARQAKAAASAHETALAVM
                     VPPSAIEANRARRNSLARANCLGQTSAAIAEAEAEYERMWARDADAMHAYAGASADAS
                     RLTPFTSPPATVEPASGDTWALRSAPELISAGHQVMSRVQEALQAISSPPTTFDMPLS
                     PVTSSLSKLSSLSAPSDTAIKHLNSLNKAAAVRWLLPNQGGARGAPITVRLGRAMPIG
                     SASVPRAWATAAAPIPVYRQTVAQRRPSEDGSRRVPVTADRGSRPARD"
     gene            complement(6260..7036)
                     /locus_tag="VFI55_08160"
     CDS             complement(6260..7036)
                     /locus_tag="VFI55_08160"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HET7074037.1"
                     /translation="MVDLTRRLAGRVAVVTGGGSGIGLAAARRMNAEGATIVVGDVDR
                     DAGAAAAEELSGLFVPTDVCDEAAVNALFDTAAQSYGWVDIAFNNAGISPPEDDLIEN
                     TELEAWQRVQDVNLKSVYLCCRAALRHMVPAGRGSIINTASFVAVLGSATSQISYTAS
                     KGGVLAMSRELGVQFARRGIRVNALCPGPVNTPLLQELFAKDPERAARRLVHVPLGRF
                     AEPDEIASAVAFLASDDASFITASTFLVDGGISSAYVTPL"
     gene            complement(7036..8403)
                     /locus_tag="VFI55_08165"
     CDS             complement(7036..8403)
                     /locus_tag="VFI55_08165"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7074038.1"
                     /translation="MNATQLINPATEEVLRSVELFDAAAVDDAVGRAQVAQRRWARLS
                     PAERAAGLRAFAAVVDAHVDELAALEMANSGHPIASAEWEAGHVRDVLTFYAASPERL
                     SGKQIPVAGGLDITFNEPLGVIAVITPWNFPMMIASWGIAPALAAGNAVLVKPAEATP
                     LTTIRLGELAAEAGLDADLLQVLPGKGAVVGDRFVTHPDVRKIVFTGSTEVGKKVMAG
                     AAAHVKRVTLELGGKSANIVFADCDLERAAATAPAGVFDNAGQDCCARSRILVQRSVY
                     DRFMELLEPAVRGVAVGDPGSRDTEMGPLVSAAHRDKVASYVPDGAPVAFRGTAPSGR
                     GFWFPPTVLTPDRADRSVTDEIFGPVVTVLAFDDEADAIALANDTAYGLSGSIWTDDL
                     SRALRVSRAVESGNLSVNSHSSVRYNTPFGGFKQSGLGRELGPDAPLHFTETKNVFIA
                     IKEEA"
     gene            complement(8400..9134)
                     /locus_tag="VFI55_08170"
     CDS             complement(8400..9134)
                     /locus_tag="VFI55_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020728286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET7074039.1"
                     /translation="MNASRPGPVIGLTSYLERVQSDGWDVQAGYLSANYFQGVIAAGG
                     IALLLPPQPSDAGVAERVLDRLDGLVITGGYDLDPAAYGQHPHPATDQPRTARDALEF
                     ALLQGALERGLPVLGICRGIQMLNVAFGGTLHQHLPDVLGHSGHRAGGGVFTPLPVRT
                     VAGTRLAALLGESAEARCYHHQAVDKVGEGLVVSAWDADGVVEAVELPGESFALAVQW
                     HPEQSLDDLRLFTAIVEAARSYADSR"
     gene            complement(9121..10512)
                     /locus_tag="VFI55_08175"
     CDS             complement(9121..10512)
                     /locus_tag="VFI55_08175"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HET7074040.1"
                     /translation="MAHKEHAEGAAKGLLSLAALEQLVGIGAVDTVIVAFTDMQGRLV
                     GKRVAAGLFVEDVAAHGAECCSYLLAVDVDMNTVAGYAMSSWDTGYGDMVMLPDLSTL
                     RLIPWLPDTALVVADLGWADGSAVAVAPRAILRRQLDRLGERGLIADVATELEFIVFD
                     HTYRQAWADGYRGLTPASDYNIDYAILASSRMEPLLRDIRRGMQGAGLRFEAVKGECN
                     RGQQEIGFRYDQALVTCDNHAIYKNGAKEIADQHGKSLTFMAKYDEREGNSCHIHLSL
                     RDQDSTAVFADSTRPHGMSSTFCSFVAGLLATLPELTLFYAPNVNSYKRFADGSFAPT
                     ALCWGLDNRTCALRVVGHGRNTRVECRVAGGDVNPYLAVAALIAGGLYGIERGLEPPE
                     PYPGNAYEATGVARLPATLAEAATAFRESTLAREAFGDDVVAHYLNNARVELAAFNAA
                     VTDWERMRGFERF"
     gene            complement(10522..11073)
                     /locus_tag="VFI55_08180"
     CDS             complement(10522..11073)
                     /locus_tag="VFI55_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7074041.1"
                     /translation="MMTTLDGFPVPVALAGPEKGSVVVILGDEQRAPVAYDAVCERLH
                     TASLRTVVIGLDRRLTAKSVVGILDALGIGWAVVVGDRAGAEIAWELVATRLGRFVGL
                     VVIDRGHPRVADSNGVVLDEQCPPVETGTTVLVSSPAARAVARASQQYVYAEYRVVDL
                     ARRTVQESTAQLAAEIVLRTTTW"
     gene            complement(11170..12669)
                     /locus_tag="VFI55_08185"
     CDS             complement(11170..12669)
                     /locus_tag="VFI55_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HET7074042.1"
                     /translation="MSGALLVAGTSSDAGKSLVVAGLCRLLARRGARVAPFKAQNMSN
                     NSVVTVEGGEIGRAQAIQARAAGLSPSVRFNPILLKPGSDRTSQLVIRGQVADTVTAA
                     GYMAYRDRLAGIVGDELSRLRDEFDAVICEGAGSPAEINLRATDLANMGLARAANLPV
                     LVVGDIDRGGLLAHLFGTVAVLDPDDQALIAGFVVNKFRGDLALLEPGLRQLCALTGR
                     PTYGVLPYSDELWLDAEDSLSVVAHRVVGTPAPPRGDDWLRVAAIRLPRISNSTDVEA
                     LACEPGVLVRWVTDPADLADTDLIVLPGSKATVADLTWLRQRGLAGAIAGHAEAGKPV
                     LGICGGLQMLCRRIEDTVESGAGEVVGLGLLDADIVFDRAKVLRRWQQPLTGYEIHHG
                     RLARCAQEAWFAVDSEPQGIVRDAVFGTHWHGLLDNDDFRRAWLTRVADAAGRSGFVI
                     AETNVAARRDAQLDLIADLLAAHLDVDAVLGLLDGPPPRRPHIASQLRP"
     gene            complement(12686..13543)
                     /gene="map"
                     /locus_tag="VFI55_08190"
     CDS             complement(12686..13543)
                     /gene="map"
                     /locus_tag="VFI55_08190"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET7074043.1"
                     /translation="MPARTALSPGVISPTLTVPSRIPRPEYVGKPTAKEGSEPWVQTP
                     EVIEKMRIAGRIAAGALAEAGKAVSPGVTTDELDRIAHEYMVDNGAYPSTLGYKGFPK
                     SCCTSLNEVICHGIPDSTVIADGDIVNIDVTAYIDGVHGDTNATFLAGDVSEEHRLLV
                     ERTREATMRAINAVKPGRALSVVGRVIESYANRFGYNVVRDFTGHGIGTTFHNGLVVL
                     HYEQPAVTTIMQPGMTFTIEPMINLGALDYTVWDDGWTVVTKDRKWTAQFEHTLLVTD
                     TGVEILTLP"
BASE COUNT         2176 a         4692 c         4675 g         2183 t
ORIGIN      
        1 gaatcccagg cgctgctggg ctatcagctg tggttgaccg tgcatcaacg tcttggtggc
       61 gatcaaggtt tgggggcggc atgagtaagt cagcgggcga atacctgtcg tcggggccgc
      121 gcttcgccga gggtgaggtc atcgagcgga tcgagtcgtt ccgcaggcgc cgcccgcggt
      181 tgctcgacga ccacgtgact ctggcgcacg gagcgggtgg gaaggcctct gcggcgctgg
      241 tggacgcggt gttcctggag gcgttccgca atccgctgct ggagtcgctg ggcgacagtg
      301 cggtcctgac gttgcccagc ggcgaacgcc tggcgatgtc caccgattca ttcgtggtgc
      361 agcccaggcg atttcccggc ggctccatcg gcgaactcgc cgtgcacggc acctgcaacg
      421 acctcgcgat ggccggcgcc gtgccgtcct ggatctcggc ggcgttcgtg ctggaagaag
      481 gcttcccgat cgccgaactg aaagagatcg tcgccgacat ggcggccgcg gccgcgaacg
      541 cgggtgtgca cgtcgtcacc ggcgacacca aggtggtggc caagggcgcc gccgacggcc
      601 tgttcatcac caccaccggc gccgggatca ttcccgcggg acgcacgctg tcgcccgcat
      661 ccgtgcgcgc cggtgacaag gtgctgttgt cgggctcgat gggcgatcac ggcatggccg
      721 tgatgctcgc ccggggcgat ctggccatcg aggccgacat cgcttccgac accgcgagcc
      781 tcagcccgtt ggtggaactg ctgatggcag ccgccccgtc caccagatgg ctgcgtgatg
      841 cgacgcgggg cggggtgggc accgtgtgca acgaactggc tcaggccagt ggccttggcg
      901 tgctgctgga cgaggagcga ttaccggtgc ggcccatggt gaatggcgcg tgcgagttgc
      961 tcgggatcga cccgctctac gtcgccaacg agggcaagtt cgtcgccgtc gtcgcgcccg
     1021 aggaggccga cgccgggctg gccgcgctgc ggtcgcatcc gctgggcgcc gaggcggccg
     1081 aagtcgggga aatcctcacc gaaccggccg aaagcgtggt gctgcgtacc gggttcggcg
     1141 gaacgcggat cgtcgacatg ctcgtcgggg acccgctgcc gcggatctgc tgagtgctgc
     1201 tgacaggagg gacctgaaca tgtgtcttgg gattccgggc cggatcgtcg aaatcaccga
     1261 tcctgccaac tatttggcga aggtcgacgt cagcggcgtg caacgcacca tcagcgtgcg
     1321 actgctggag gacgacatgc ccgagcccga cgagtgggtg ctggtccacg tcggcttcgc
     1381 gatggccaag atcgacgaga ccgaggcctt actcaccctg gcggccgtca agaagctcgg
     1441 cgaggcctat accaccgaaa tggaagcctt cgactcgtcg gccatcgtct aaaggaaaaa
     1501 gcacatgaaa ttcgttgacg agttccgtga cccggccgca gcccgcaaat tgctgggcgc
     1561 catcgagcac ctggccggtg agcgcgacca tttcaagttc atggaggtgt gcggcgggca
     1621 cacccacacc atttaccggc acggcatcga acacctgctg cccgacaacg tcgaactggt
     1681 gcacggcccc ggctgcccgg tatgcgtgat cccgatgggc cgcgtcgacg acgccatgtg
     1741 gctggccggc cagcccgacg tcatcttcac ctgcttcggc gacatgatgc gggtgcccgg
     1801 atcgtccggc agcctgctgg acgccaaggc gcgcggcgcc gacgtgcggt tcgtctactc
     1861 cccgctggat gcgctgaaga tcgcgaccga caaccccggc aaacaagtgg tgttcttcgc
     1921 catcggtttc gagaccaccg ccccgtcgac cgcggtgacg ctggtgcggg cacgcgactt
     1981 gggcctcacc aatttcagtg tgttctgcaa ccacgtcacg atcgtgccgc cgatcaaggc
     2041 catcctcgaa tcgccggacc tgcggctgtc gggtttcatc ggcccggggc acgtgtcgac
     2101 ggtggtgggc aaccggccgt accggttcgt gccggaggtg tatcgaaagc cattggtggt
     2161 ggccggtttc gagccgctgg acatcctggc ggcggtcgcg atgctgctgc gccagatccg
     2221 cgagggccgc tgcgaggtgg aaaaccagta caagcgcgtg gtacccgagc acggcaatcc
     2281 ggcggcgctg gcgctgatgg gcaaggtgtt cgcgctgcgc ccgcacttcg aatggcgcgg
     2341 gctggggttc atctcgcaaa gtgcgctgcg gctgcacgac gacttcgcgg agttcgacgc
     2401 cgagctgcga ttctcgatgc cgggtgtgcg ggtcgccgat ccgaaagcct gccagtgcgg
     2461 tgaggtgctc aagggcgtgc tcaagccttg ggagtgcaag gttttcggca ccgcgtgcac
     2521 cccggagacc ccgatcggaa cgtgcatggt gtccccggag ggggcatgtg cggcctacta
     2581 caacttcggc cgcatgcacc gcgatgccgt caaactcgtc ggacagtcag gacgggtgtg
     2641 agtcgttgac cgatgcccgc ggcgccgaga tgttcgcacg ctacgcctac gcgcccaacg
     2701 cgctgggcta ctgtggtccc ccgctcggcg ccaccctgcg cgacggctcg gtcgacgacg
     2761 ttcgcaaggc cgcgacgaag ttttccgggg cctggcccta cctgcgggtg ctgtccgcgc
     2821 tgaccggtat cgccgacccg ttggattacc ggctggtcga gtcgtattgg ctcggcggcg
     2881 gcgtcggcgc ggacgtcgac cccgaagact tcttcgacag gttgctggcg atcatcggcc
     2941 cacaggccgg acgctactgg tcgcacctga ccgcggacct ggcgcgcgag gcggcgggca
     3001 accactgttt ccacgtgttc ggcgtctatc cctggacacg gttcctgggg cggggcgagc
     3061 atccgctgaa tgtgctggac aattgccgga tcacttgggg cacagtcgtt tcccgcgtcg
     3121 acgacaacgt cgaggtgatg tgccagcggc tcgtctggga cggccaagcg ctgacgctgt
     3181 ccgaaccttc gttgcgctta ttggacgtct gggccgacgg ctacagcgcg gtgcccgagg
     3241 tgggcgtcgc ggatgaggtc gcggtgcact ggggccgcct gtgcggccgg ctgtcgcggg
     3301 agcaagttcg cgcgctgcgc gacagcacgg accgacagct ccgggtgacc agccagcggc
     3361 tggcccgggc cctactgcca gactgaccgt cgtgcccggt tccatctgcg acgtactgcc
     3421 cgccgcggcc gcgctgctgg gcgctccggg agcggtcgac actctggggt tgaccgattg
     3481 ggtcgacggc cgggtcgagc gcgtagtggt aatgctggtc gacggaatgg gctggcacct
     3541 gttacccaag ttggccccca gtgccccgct gcttgcctcc gtgctctgcg gcggcatcgg
     3601 ccgcctcagc gaactcagct gcaccttccc gtcgaccacc ccgagcagcc tggtgtccct
     3661 gggcaccggg gcccgccccg gcgcgcacgg catcctgggc ttcgcgctca acatccccgg
     3721 caccgacagg gtgctcaacc acattcggtg gcgcgacgac ccgccctatg ccgaatggca
     3781 gccgctgcca acctggttcg agcggctcga gcaagccggc gtgagcgcgc gcgccgtgct
     3841 gcccggggcg ttcatcggca gtgggttgac agacgcggcg tatcgcggcg cccgctttcg
     3901 ccccacggat gcaaccgacg actatgcgcg gacgctggcc gacgaggtga acgccgggcc
     3961 gggtctgatc tacggctata ccaccgagct ggacaccgcg gcccacgtct tcggcatcgg
     4021 atcccccagc tggcacacgg cggcggctca cgtcgatgag ctgctgcgcc ggctgctcga
     4081 gacactgccc gcgaaagcgg tgctgctggt gaccgccgac cacggcgggc tcaacgtgcc
     4141 gccggacgcc cgcgccgacc tcgacaccga cccacggctg gccgcggggg tgcgggtggt
     4201 tgccggcgaa ccgcgggtgc gttacctgca caccgagccg cgtgccgccc cggacgtgca
     4261 ggccacctgg accgaggtgc tcaacggccg cgtacaggtt tacctccgcg aggaagccgt
     4321 ggccaccggg atgttcgggc cggtcaaccc gtcgcacctg gcgcgcatcg gggacgtcgt
     4381 cgtcgtatgc accggcgact ccgccgtgtt ggcaaccgcc cacgagccac cggaaacggc
     4441 ccgcctcata ggttttcacg gcgcggccac cgcagccgaa atggccattc cgctcatcgc
     4501 tttcggatcg gcccaggtgg atgacgaccc gccggtaatg cacaccggac tttgatcgtt
     4561 ctcacagctc gctgcgggtg tcggccaagt tccgtgttgt acattcgggc cactgtgact
     4621 ttccgtggcg gcagaagaac tcgacgttgg caggtggcca ttgcgatcgt cgcggcgctg
     4681 actctgttcg cagccgtcac caccggttgg gcggcgcggg gatccgcgta cgcttcgtcg
     4741 gcactgtggc agccggcgac cgtgtcgcat caagcatcgc atgtcggcgt gcaggtggac
     4801 catctccaac gtgccgacca cgccccaccc cggacgcacc agaaaccaac caaaaacgcg
     4861 tggatgaccc gcgatcgacc gccgacatgg acgccactat cgccacaatc ggtcctctcg
     4921 ccgttgccgg tttcgtttgc tgcacaatgg ctttcaccgg acgtggcccc cgcggttgtg
     4981 cccacgacgg gccgttccgg ccaggacata ttggccgagt tctgcgtcgc acgtcgctga
     5041 ccggtccgcg ccgggccgtg tttcagcatg cgtgcgggca cttcaccatg agaagtcgct
     5101 aacagcaacc cgtctggtcg acttgcgcac gcgcttgtgg cgggggcaaa gctcgtaacc
     5161 cccgtgtcta tgtcctagag gaagagtcga tgttttccag agcaaccaat accgccgtag
     5221 tcgggcaggg gcagcaatga atttcgccac gataccgccg gaaatccact ccgggcgaat
     5281 gcatgccggt ccaggttcgg gatcgctgac cgaggccgtg acggcctggg aacggttggc
     5341 cgtccggctc tccaccgcgg ctgcggacta ccgggcggtg acgtcaaagc tggcggcatg
     5401 gggcggcccg gcgtcgacgg cattgaccga ggccgcggcg ccctacattg tgtggctcgc
     5461 cgccaccgcc gcgcacgctg agcacgcggc ccgccaggcg aaggcggcgg ccagtgccca
     5521 cgagacggcg ttggcggtga tggtgccccc gtcggcgatc gaggccaatc gcgcgcggcg
     5581 aaactcgctg gcgagggcga attgcctggg gcaaaccagc gcggcgatcg cggaggccga
     5641 ggccgagtac gagcggatgt gggcgcggga cgccgacgcc atgcacgcct acgccggcgc
     5701 ctcggccgac gcctcgaggc tgacaccatt cacctcgccg cccgccacgg tggaacccgc
     5761 cagcggggac acctgggccc tgagatcggc gcccgagctc atctccgccg gccaccaggt
     5821 gatgtcaagg gtccaggaag cactgcaggc gatttcgtca cccccgacaa cattcgacat
     5881 gcccctttcg ccggtgacct cgtccctgtc gaaactcagt tcactgagcg cgccgtcgga
     5941 caccgcgatc aagcatctga attcgctcaa taaggcggcg gcagtgaggt ggctgctgcc
     6001 gaaccagggt ggtgcccgcg gcgcgccgat caccgtgagg ctcggtcgcg cgatgccgat
     6061 cgggtcggca tcggtaccgc gcgcctgggc gacggcggcg gcgccaatcc ccgtctaccg
     6121 tcaaaccgtt gcgcagcgcc ggccgtcgga ggacggttct cggcgtgtgc cggtcacagc
     6181 cgaccgcggg agccggccgg ctcgcgacta gccggcgagc atgtcgccgt caccgctgac
     6241 aaccggcaac aactcgcgat tacagcgggg tgacgtaggc ggagctgatg ccgccgtcga
     6301 ccaggaacgt cgaggcggtg atgaacgatg cgtcgtcact ggccaaaaat gctactgcgg
     6361 aagcgatttc gtcgggttcg gcgaagcggc ccaacggcac gtgcaccagc cggcgggcgg
     6421 cccgttcggg atctttggcg aaaagctctt gcagcagcgg ggtgttcacc ggtccggggc
     6481 acagcgcgtt gacccggata ccccgccggg cgaattgcac gcccagctca cgcgacatgg
     6541 ccagcacccc gcccttggag gcggtgtagg agatctgcga ggtcgccgat cccagtaccg
     6601 cgacgaacga ggcagtgttg atgatcgagc cccttccggc gggcaccatg tgccgcaacg
     6661 ccgcccggca gcacaggtac accgatttga ggttgacgtc ttggacccgt tgccacgcct
     6721 caagttcggt gttttcgatc aagtcatcct cgggtggtga gatgccggcg ttgttgaacg
     6781 cgatgtcgac ccagccgtag gactgggcgg cggtgtcgaa cagggcgttg accgcggcct
     6841 catcgcaaac atcggtcggc acaaacaaac cggacagctc ctcggccgcc gcggcgcccg
     6901 cgtcacggtc gacgtcgccg acgacgatgg tcgcgccttc ggcgttcatc cgccgcgcgg
     6961 cggccaaccc gatgccgctg ccaccgccgg tgacgaccgc cacgcggcct gccaaccgtc
     7021 gagtcaggtc caccatcagg cctcctcttt gatcgcgatg aacacgttct tggtctccgt
     7081 gaaatgcagt ggcgcatcgg gtcccagctc acgcccaagc cccgattgct tgaagccacc
     7141 gaacggcgtg ttgtaacgga ccgacgagtg tgagttcacg gacaggttgc cggactcgac
     7201 cgcccgcgac acccgcagcg cgcgggacag atcgtcggtc cagatcgatc ccgacaggcc
     7261 gtaggcagtg tcgttggcca gcgcgatggc gtcggcttcg tcgtcgaacg ccaacacggt
     7321 gacgaccggg ccgaagatct cgtcggtgac gctgcggtcg gcgcggtccg gggtgagaac
     7381 cgttggtgga aaccagaatc cgcgaccgga tggtgccgtg ccgcgaaacg cgacgggggc
     7441 gccgtcgggc acataggaag ccaccttgtc gcggtgcgcg gcagacacca gcggacccat
     7501 ctcggtgtcg cgggatccgg ggtccccgac ggcgactccg cgcaccgccg gctccagcag
     7561 ctccatgaac cggtcatata cgctgcgctg caccaggatc cgactacggg cacagcaatc
     7621 ctgcccggcg ttgtcgaaca ccccggcagg agcggtcgcc gccgcgcgct ccaggtcaca
     7681 gtcggcgaag acgatgttgg cgctcttgcc gcccagttcc agcgtcaccc gcttgacgtg
     7741 ggccgccgcg ccggccatca ccttcttgcc gacctcggtg gacccggtga acacgatctt
     7801 gcgaacgtcc gggtgggtga cgaacctgtc cccgaccacc gcgcccttac ccggcaacac
     7861 ttgcagcagg tccgcgtcga gcccggcctc ggcggccaac tcgccgagcc ggatcgtggt
     7921 cagcggcgtc gcttccgccg gtttgaccag cacggcgttg ccggcggcca gggccggcgc
     7981 gataccccag gacgcgatca tcatcgggaa gttccacggc gtgatcaccg cgatcacgcc
     8041 cagcggttcg ttgaaggtga tgtcgagacc gcccgcaacg ggaatctgct tgccggacaa
     8101 gcgttccggg ctggccgcgt agaaagtcaa cacgtcgcgg acatgtccgg cttcccactc
     8161 ggcggacgcg atcgggtgtc ccgaattggc catctcgagg gcggccagct cgtcgacatg
     8221 ggcgtcgacg acggcggcga acgcgcgcag gcccgccgcc cgctccgccg gcgacaaccg
     8281 cgcccagcgc cgctgggcca cctgcgcccg gcccaccgcg tcgtcgaccg cggcggcatc
     8341 gaagagctcg accgagcgca gcacttcctc ggtggcaggg ttgatgagtt gtgtcgcgtt
     8401 catcggctgt ccgcgtacga ccgcgcggcc tcgacaatcg ccgtgaacag ccgcaggtcg
     8461 tccagggact gctccggatg ccactgcacc gcaagcgcga agctctcccc gggtagctcc
     8521 accgcctcga ccacgccgtc ggcgtcccat gcgctgacca ccagaccctc accgactttg
     8581 tcgacggcct ggtggtggta acaccgcgcc tcggccgatt cgccgagcaa tgcggccagc
     8641 cgggttccgg ccaccgtgcg caccggcaac ggggtgaaca ccccaccgcc cgcccgatgc
     8701 ccgctgtggc cgaggacgtc cggcaagtgc tgatgcagcg tcccgccgaa cgcgacattg
     8761 agcatctgga tgccccggca gatgcccagc accggcagtc cccgctcgag ggcgccctgc
     8821 aacaacgcga attccagggc gtcgcgggcg gtgcggggct ggtcggtggc cggatgcggg
     8881 tgctggccgt aagcggccgg gtcgaggtcg taacccccgg tgatcaccag gccgtcgagg
     8941 cggtcgagca cccgctcggc aacgccggcg tcggacggct gcggcggcag cagcagtgcg
     9001 atgccaccgg ctgcgatcac gccctggaag tagttggcag acagataacc cgcctggacg
     9061 tcccagccgt cgctctgcac acgctccagg tagctggtca aaccgatgac cggtcccggc
     9121 ctagaagcgt tcaaaacccc gcatcctttc ccagtcggtg acggccgcgt tgaacgccgc
     9181 caactccacc cgggcgttgt tcagatagtg cgcgacaacg tcgtcgccga acgcttcccg
     9241 tgccagcgtc gactcccgga acgcggtggc cgcctcggcc agcgtggcgg gcaggcgcgc
     9301 gacgccggtt gcctcatagg cattcccggg gtacggctcg ggtggctcga ggccccgttc
     9361 gataccgtac agcccgccgg cgatcagtgc cgccaccgcc aggtagggat tgacatcgcc
     9421 gccggcgacc cggcattcga cccgggtgtt gcgtccgtga ccgacgaccc gcagtgcgca
     9481 ggtgcgattg tccagtcccc agcacagcgc cgtgggtgcg aagctgccgt cggcaaatcg
     9541 cttgtaggag ttgacgttcg gcgcatagaa cagggtcagt tcgggcaacg tggccagcag
     9601 gcccgcgacg aaactgcaaa aagtggacga catgccgtgc ggccgggtgc tgtcggcgaa
     9661 taccgcagta ctgtcctggt cacgcagcga caggtggatg tggcagctat tgccttcgcg
     9721 ctcatcgtat ttcgccatga acgtcaggct cttgccgtgc tggtcggcga tctccttggc
     9781 gccgtttttg tagatggcgt ggttgtcgca ggtgaccagc gcctggtcgt aacggaatcc
     9841 gatctcctgc tggccccggt tgcattcgcc cttgacggcc tcgaaccgca gcccagcacc
     9901 ctgcatgccc cggcggatgt cgcgcagcag cggctccatg cgcgacgacg ccaagatcgc
     9961 gtagtcgatg ttgtagtcgc tggccggggt caggccccga tagccgtcgg cccaggcctg
    10021 gcggtacgtg tggtcgaaca cgatgaattc cagctcggtg gcgacgtcgg cgatcagtcc
    10081 gcgctcgccc agtcggtcga gttggcggcg caggatggcc cgcggtgcga cggcgacggc
    10141 gctgccgtcg gcccagccca gatcggcgac caccagggcc gtgtcgggca gccatggaat
    10201 gagccgaagc gtggacagat ccggcagcat caccatgtcg ccgtagccgg tgtcccagct
    10261 cgacatcgcg tagccggcca cggtattcat gtcgacgtcg acggcgagca gatagctaca
    10321 gcactcggcg ccgtgcgcgg ccacgtcctc gacgaacagc ccggcggcca cccgcttgcc
    10381 gaccaacctg ccctgcatgt cggtgaacgc cacgatcacg gtgtccaccg cgccgatccc
    10441 caccagttgt tccagcgcgg ccagcgacaa cagacctttc gcagcgcctt ctgcgtgttc
    10501 tttatgagcc atcaaggccg gtcaccaggt cgtggtgcgc aacacgatct cggcggccaa
    10561 ctgtgcggtc gactcctgca cggtgcgccg ggcgaggtcc accacgcggt attcggcgta
    10621 gacgtactgc tggctggccc gggccaccgc ccgagcggcc ggggagctga ccagcacggt
    10681 ggtgccggtc tccaccggag ggcattgttc gtccagcacg acgccattgg aatcggccac
    10741 ccggggatgt ccgcggtcga tcaccaccag gccgacgaac cggcccagcc gcgttgccac
    10801 cagttcccag gcgatttcag cgccggcgcg gtcgccgacc acgacggccc agccgatccc
    10861 cagcgcatcg aggatgccga ccaccgactt ggcggtcagc ctacggtcga ggccgatgac
    10921 gacggtgcga agcgacgcgg tgtgcaggcg ttcgcacacc gcgtcgtacg cgacgggggc
    10981 acgttgctcg tcacccagaa tgacgacgac ggagcccttc tcgggcccgg ccaacgctac
    11041 cgggaccgga aaaccatcga gcgtggtcat catcgagtgc actcacgcac gctacagcca
    11101 atggcctgcg cgcgcgaggg tttgccgctc atcggccctc gtcgccgccg tgatggcgcg
    11161 gcgaatgcct tacgggcgca gctgactcgc gatgtgtggg cgcctcggcg gcggcccatc
    11221 gagcaggccg aggacggcgt cgacatcgag gtgggccgcc agcagatcgg cgatcaggtc
    11281 cagctgggca tcgcgccgcg cggcgacgtt ggtttcggcg ataacgaatc cactccggcc
    11341 ggccgcgtcg gcgacccggg tcagccacgc gcgacggaag tcgtcgttgt cgagcaggcc
    11401 gtgccaatgg gtgccgaaga cggcgtcgcg cacgatgccc tgcggttcgg agtcgaccgc
    11461 gaaccaggcc tcctgggcgc atcgggcgag ccggccgtga tggatttcgt atccggtgag
    11521 cggctgctgc cagcggcgca ggactttggc ccggtcaaag acgatgtcgg cgtccaacaa
    11581 ccccaggccg acgacctccc ccgcgccgga ttcgactgtg tcttcgatgc gacggcacag
    11641 catctgcagg cccccgcaga tgcccaggac cggcttgccg gcctcggcat gaccggcgat
    11701 cgcgccggcc agtccgcgct gccgcagcca ggtcaggtcg gcgacggtgg ccttgctgcc
    11761 cggcagcacg atcaggtcgg tgtcggccag gtcggcgggg tcggtgaccc agcgcaccag
    11821 cacgccgggc tcgcatgcca gcgcctcgac gtcggtggag ttggaaatcc ggggcagccg
    11881 gatggcggcg acccgcagcc agtcgtcgcc gcgcggtggc gcgggcgtgc cgacgacccg
    11941 gtgggcgacc accgacagcg aatcctcggc gtccagccac agctcgtcgc tgtagggcag
    12001 cacgccatag gtcgggcggc cggtcagggc acacagttgc cgcagccccg gttccagcag
    12061 cgcgaggtcg ccgcggaact tgttgacgac gaagccggcg atcagcgctt ggtcgtcggg
    12121 atcgagcacc gcgaccgtcc cgaacagatg cgccagcagg ccgccgcggt cgatgtcgcc
    12181 gaccaccagc accggcaggt tggcggccct ggccaacccc atgttggcca aatcggtggc
    12241 gcgcaggttg atttcggccg gcgagccggc cccctcacag atcaccgcgt cgaattcgtc
    12301 gcgcaggcgc gacaattcgt ctccgacaat gccggcgagc cggtcgcgat acgccatata
    12361 gcctgccgcc gtgaccgtgt cggcgacctg cccccggatc accagctgcg acgtccggtc
    12421 gctgccgggc ttgagcagga tcgggttgaa tcgcacgctg ggcgaaagcc ccgccgcgcg
    12481 ggcctggatc gcctgggcac ggccgatttc gccgccttcg acggtgacca ccgagttgtt
    12541 ggacatgttc tgtgccttga acggcgccac ccgggccccg cggcgcgcca acaaccggca
    12601 caaaccagcg accaccaggg atttccccgc gtccgagctg gtgcccgcaa ccagcagcgc
    12661 cccgctcaca ggctggccgg cagcctcatg gcaacgtcag gatttcgacc ccggtatcgg
    12721 tgaccagcag ggtgtgctcg aactgcgcgg tccacttgcg gtccttggtc accaccgtcc
    12781 agccgtcgtc ccagaccgtg tagtccagcg cgccgaggtt gatcatcggt tcgatggtga
    12841 acgtcattcc cggctgcatg atcgtcgtca cggccggctg ttcgtagtgc agcaccacca
    12901 gcccgttgtg aaacgtggtg ccgatgccgt ggccggtgaa gtcacgaacg acgttgtacc
    12961 cgaaccggtt tgcatacgac tcgatgacgc ggccgacgac cgacagcgca cgaccgggtt
    13021 tgacggcgtt gatcgcgcgc atcgtcgcct cgcgggtccg ctcgacgagc aaccggtgct
    13081 cctcggagac atcgccggcc agaaaggtcg cgttggtgtc gccgtgcacc ccgtcgatgt
    13141 aggcggtgac gtcgatgttg acgatgtcgc cgtcggcgat caccgtcgag tcggggatgc
    13201 catgacagat cacctcgttg agcgacgtgc agcacgactt cggaaagccc ttgtagccca
    13261 gcgtcgaggg gtaggccccg ttgtcgacca tgtactcgtg ggcgatgcgg tccaactcgt
    13321 cggtggtcac ccccggggaa accgctttgc ccgcctccgc gagcgcaccc gccgcgatcc
    13381 ggccggcgat ccgcatcttc tcgatcacct ccggcgtctg cacccacggc tcgctgccct
    13441 ccttggcggt gggcttgccg acgtattccg gacgcgggat gcggctgggc accgtcaggg
    13501 tcggggatat cactcctggg gaaagcgcgg tgcgagctgg catgtcgcca gcttaatagc
    13561 ggctttcctc aggagggcct gggcatcgag gtgggggcac ccccagccgc ggagcggcga
    13621 gggggagcat cggcggcgcc cagtgtgaac gtggggcggg aattcgtccg atttcccgcc
    13681 ctcagcgcac gcccgacgag gcgcccggcg acgaagcggc ccgtgc
//
LOCUS       DASSGQ010000185         2658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_530082,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000185 DASSGQ010000000
VERSION     DASSGQ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530082"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1153)
                     /locus_tag="VFI55_08195"
     CDS             complement(122..1153)
                     /locus_tag="VFI55_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fused (3R)-hydroxyacyl-ACP dehydratase subunits
                     HadA/HadB"
                     /protein_id="HET7074044.1"
                     /translation="MTSAAEASTLESRVGHYYQMDDPYLVGREKVREYARAVQDRHPA
                     HWDVAAAAELGYSGLVAPLTFTSIPGMMANRRLFESVVVGYDTYVQTEEVFEQHRPIV
                     AGDELHTDVELSSVRRIAGRDLITVTNTFTDTAGERVHTLHTTVVGVTADDIDPAIKA
                     VVQNAMMHDAYGLPIGESDTAYHRTVRPEGDVRIAEGGMTRTPGTPSFDDVKVGAELP
                     VRHARLSRGDLVNYSGVAGDANPIHWDEDIAKAAGLPDVIAHGMLTMGLGAGFFSAWS
                     GDPGAVSRYAVRLSAPAIVSATDGADIEFSGRVKSLDEATRSGVVVVAAKSDSRKIFG
                     LATMHVRFR"
     gene            complement(1396..1677)
                     /locus_tag="VFI55_08200"
     CDS             complement(1396..1677)
                     /locus_tag="VFI55_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HET7074045.1"
                     /translation="MIGAAVVTNAGIATADPANDAYLAQLRGLGFSWPPGHEDALIGT
                     AFLICDDLWWGWTPDQIAQHVHANLDPRKVTLGDITSMVNVARATYCPT"
     gene            complement(1948..2490)
                     /locus_tag="VFI55_08205"
     CDS             complement(1948..2490)
                     /locus_tag="VFI55_08205"
                     /inference="COORDINATES: protein motif:HMM:NF023900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074046.1"
                     /translation="MAREFGDEQLELDNEINGLDGRIMSRFDQLGAVGPAATAGLGQA
                     GSVGALSVPQGWTVAAPAIRLAAMASPATNLDATPDVFAGSPASLFSEMVLASMAGRA
                     IYGTLNPGGRERIAATARACPAPPPPRSPSGGAPLVAEMREVAELLGKLGALRDSGIL
                     TCEEFNEQKRRLLGTAPASI"
BASE COUNT          450 a          846 c          880 g          482 t
ORIGIN      
        1 gcagatcgcg gcctgacgcc gtccaatcgg cgtcaggaca atccgacatg ttgctgcgga
       61 gaatgcggca ggtaacggac ccgacggctg ggcgaccggg ccgccaccag gaccgccggg
      121 gttagcggaa gcggacgtgc atcgtcgcca ggccgaagat tttccggctg tcggatttcg
      181 cggcgaccac gacgacaccg ctgcgggttg cttcgtcgag cgacttgacc cggccgctga
      241 attcgatatc cgcgccgtcg gtggccgaca cgatcgcggg cgcggacagc cgcaccgcgt
      301 agcggctcac cgcaccgggg tcgcccgacc atgcggaaaa gaatccggcg cccaaaccca
      361 tggtgagcat gccgtgggcg atcacatcgg gcagccctgc cgctttggcg atgtcctcgt
      421 cccagtgaat cgggttggca tcgccggcca cgccggaata gttcaccagg tcgccgcggg
      481 acagtcgggc gtggcgcacc ggtagctcgg cgccgacctt cacgtcgtcg aaggacggcg
      541 tccccggcgt acgggtcatg ccgccctcgg cgatccgaac atcgccctcg ggccgcaccg
      601 tccggtggta cgcggtatcg gattcgccga ttgggaggcc gtacgcgtcg tgcatcatcg
      661 cgttctgtac gaccgccttg atcgccggat cgatatcgtc ggcggtgacg ccaacgacgg
      721 tcgtgtgcag ggtgtgcacc cgctcgccgg cagtgtcggt gaaggtgttg gtcacggtga
      781 tcaggtctct gccggcgatt ctgcgcaccg acgagagttc gacgtcagtg tgcagttcat
      841 cgccggccac gatcgggcgg tgctgctcga agacttcttc ggtctgcacg taggtgtcgt
      901 agccgacgac caccgattcg aagagcctcc gattggccat catgccgggg atcgacgtga
      961 acgtcagcgg cgccaccaat cccgaatatc ccagctccgc ggcggcagcg acatcccagt
     1021 gcgcgggatg acggtcctgt accgcgcggg cgtactcgcg caccttctcg cggccgacca
     1081 ggtaggggtc atccatctgg tagtaatggc cgacccgcga ttcgagcgtc gaagcttctg
     1141 ctgctgaggt catgaatttg cccaactttt ctgtcggcct gttcgccgag gcggacccaa
     1201 gcacacacta gtcggcaatc ccaaggcaac cgtggtgtca gcggtcggtc cgggtcagcc
     1261 cgtcgatcgc gagtacggcg caccccggag cgcatctgcg cgggtgcgtt cggccatagg
     1321 acggtgccgt gccgacggcg tttcgccgtg ctgcgtgcta aaccctggac cgctaagcag
     1381 tgcggcccag cacctttagg tggggcaata ggtcgcacgg gcgacgttga ccatggatgt
     1441 gatgtctccg agcgtcactt ttctcgggtc caggttggcg tggacgtgct gggcgatttg
     1501 gtctggcgtc cagccccacc agaggtcgtc gcagatgagg aacgccgtcc cgatcagggc
     1561 gtcttcgtgc cccggcggcc aactgaagcc gaggccgcgc agttgagcaa ggtaggcgtc
     1621 attagcggga tcggcggtcg cgatcccagc gttggtaacg acggcagcgc caatcatcat
     1681 ggggacggtc aatccagcca gccagcgacg tgagggcatt tgacgcgctt cctttctccg
     1741 tggcattgaa ggtgcctaag tgtgcggggg caatgcggca caagtgtgaa gaatagagaa
     1801 agctgggaaa aacttgcgaa aggctgacca gtcactcttg gctcagcgag tcgtgtttgt
     1861 tcctgggaac gctcatgtgg ggtgaggcag ttgtgcaaac tccgaaccac gcgctgccac
     1921 acccgcacag accacaaaac gaagctgtca gatggatgcc ggcgcggtgc cgagaaggcg
     1981 tcgcttctgc tcgttgaatt cctcgcaggt cagaatcccc gagtcgcgga gtgcgccgag
     2041 tttgccgagg agttcggcga cttcgcgcat ctcggcgact agcggggcgc cgccgctcgg
     2101 tgacctgggc ggcggcggcg ccgggcatgc acgagcagtc gctgcgatgc gctctcggcc
     2161 gcccgggtta agagtgccgt agattgcccg cccggccatg ctggccagga ccatctcgct
     2221 gaacaggctt gccggactac cggcgaagac atctggggtg gcgtcaaggt tggtggccgg
     2281 cgacgccatc gcggccagcc tgatcgccgg tgccgccacg gtccagccct ggggcaccga
     2341 taatgccccg accgagcccg cctggcccag acccgctgtg gccgccggcc ctactgctcc
     2401 caactggtcg aacctgctca taattcgacc gtccaagccg ttgatttcgt tgtccagttc
     2461 aagctgttcg tcgccaaatt cacgcgccat gtcagtcccc tcgttcagaa tctcttggtc
     2521 gccttgcgct gcggagttcc atgcccctgc cgccgtggtc aactcagacg acgcgatacc
     2581 cgtactcgcg ggctccaata ggctcaaagg gtttattacg cccaggtacc cgagggttcc
     2641 ggtgagtggg ctcaacgg
//
LOCUS       DASSGQ010000186        10856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_378152,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000186 DASSGQ010000000
VERSION     DASSGQ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10856
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378152"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="VFI55_08210"
     CDS             <1..165
                     /locus_tag="VFI55_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HET7074047.1"
                     /translation="DITTSEEHDRLRGLEVELDQCWDLLRQRRALRETGGDPREAEVR
                     PPNQVEGYLN"
     gene            162..1730
                     /locus_tag="VFI55_08215"
     CDS             162..1730
                     /locus_tag="VFI55_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7074048.1"
                     /translation="MSKDPADLDVIVIGGGHNGLVAAGYLARAGLRVRLLERLGHVGG
                     AAVSAQAFDGVEVRLSRYSYLVSLLPSRIVEDLGASVRLAKRQYSSYTPDPGTAGCTG
                     LLVGRPDTFGAIGAVRDESGFAAFYQRCRLVTERLWPTLIEPLRTREQARRHVLAGGR
                     PDAATAWRAMVDEPIGHAIADAVSSDLVRGVIATDALIGTFARLDDPSLRQNICFLYH
                     VLGGGTGDWDVPVGGMGAVTTALAVAATRHGAEITTGADVYAVEPDGRVRYRIGDDEH
                     TVRGRFVLAGVTPAVLAGLLGEEPPRAAQGAQVKVNMVLRRLPRLRDNSVTPEQAFAG
                     TFHVNETWTQLDAAYTEAAAGRLPDPVPCEAYCHSLTDPGIMSAALRDSGAHTMTVFG
                     LHTPDSLFAGTGSETLGERLTDAVLASLNSVLAEPIQDLLMSDAHGRACIETTTTRDL
                     HRTLGMTAGNIFHGGLSWPFAADDDPLDTPARQWGVATAHARVMLCGSGARRGGAVSG
                     IGGHNAAMALLASL"
     gene            complement(1750..3045)
                     /locus_tag="VFI55_08220"
     CDS             complement(1750..3045)
                     /locus_tag="VFI55_08220"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET7074049.1"
                     /translation="MGDTDDTATLKYPGGEIDLEVVHATEGADGIALGSLLAKTGHTT
                     FDNGFVNTAACKSSITYIDGDAGILRYRGYPIEQLAEKSTFIEVSYLLIYGELPDKDQ
                     LAQFTKRIQLHTMLHEDLKRFFDGFPRNAHPMPVLSSVVNALSAYYPDALDPVDNGQV
                     ELSTIRLLAKLPTIAAYAYKKSVGQPFLYPDNSLSLVENFLWMTFGLPAEPYVADPEV
                     VRALDMLFILHADHEQNCSTSTVRLVGSSKANLFTSISGGINALWGPLHGGANQAVLE
                     MLETIRDSGDDVDEFVRKVKNREAGAKLMGFGHRVYKNYDPRARIVKEQADKILAKLG
                     GDDALLGIAKALEETALTDDYFIERKLYPNVDFYTGLIYRALGFPTRMFTVLFALGRL
                     PGWIAHWREMHDEGDSKIGRPRQIYCGYTERDYAAIDTR"
     gene            complement(3267..3926)
                     /gene="pdxH"
                     /locus_tag="VFI55_08225"
     CDS             complement(3267..3926)
                     /gene="pdxH"
                     /locus_tag="VFI55_08225"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HET7074050.1"
                     /translation="MAGPHEQHLQRMRVEYGSVEKDGSPDLDVDWLAEGWLALFRKWL
                     EDAERAGVAEPNAMVLATVAEGKPVSRSVLCKSADETGISFFTNYDSAKGIELAAMPY
                     ASATFPWYQLGRQVHIRGPVTKVDPRVTQDYWSKRPRGSQLGAWASHQSSPIASRAAL
                     LDQLTEVTERFADSELVPVPPNWGGYLMAPEVVEFWQGRENRVHNRIRVFHGQAERLQ
                     P"
     gene            4001..5122
                     /locus_tag="VFI55_08230"
     CDS             4001..5122
                     /locus_tag="VFI55_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase 2"
                     /protein_id="HET7074051.1"
                     /translation="MTVVPENFVPGLEGVVAFTTEIAEPDKDGGALRYRGVDIQDLVN
                     QQVTFGDVWALLVDGKFGHGLPPAEPFPLPIHTGDVRVDVQAGLAMLAPIWGYKPLLD
                     TDESTARDQLARASVMALSYVAQSARGIYQPAVPQRVIDECPTVTARFMTRWQGDPDP
                     RHIEAIDAYWVSAAEHGLNASTFTARVIASTGADVAAALSGAIGAMSGPLHGGAPARV
                     LPMIEEVERTGDARALVKSVLDSGDKLMGFGHRVYRAEDPRARVLRATAERLGAPRYE
                     VAVALEQAALAELRERRPDRAIETNVEFWAAVILDFARVPANMMPAMFTCGRTAGWCA
                     HILEQKRLGKLVRPSAIYVGPEPRSPESVEGWDRVLTSA"
     gene            5274..5732
                     /locus_tag="VFI55_08235"
     CDS             5274..5732
                     /locus_tag="VFI55_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7074052.1"
                     /translation="MAITIEPTLSPHLVVDDAAAAIDFYVKAFDAVELGRVPGPDGRL
                     IHAALRINGFTVMLNDDFPETCGGKSMTPKSLGGTPVTIHQTVTDVETKFQQALDAGA
                     TVVLPLEDQFWGDRYGMVADPFGHHWSLGQPVREVSADEIAAAMSGQTSG"
     gene            complement(5754..7454)
                     /locus_tag="VFI55_08240"
     CDS             complement(5754..7454)
                     /locus_tag="VFI55_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7074053.1"
                     /translation="MPHVITQSCCNDGSCVFACPVNCIHPTPDEPGFATSEMLYIDPV
                     ACVDCGACVNACPVGAIAPDSRLDPKQLPFIEINASFYPERPADVKLPPTSKLAPVIP
                     AAEVRALRQPLTVAIVGSGPAAMYAADELLTQHGVRVNVFEKLPTPYGLVRAGVAPDH
                     QNTKGVTRLFDRVSRHRRFRFYLNVEIGKHLSHAELLAHHHAVLYAVGAPDDRRLDID
                     GMGLPGTGTATELVAWINGHPDFTDLPVDLSHERAVIIGNGNVALDVARVLTADPDDL
                     VRTDISDHALEAFRRSMVREVVIAARRGPAHSAFTLPELVGLTGASEVVLDAADQVRV
                     ANDLGMVSDPLTENKLEILSKLCNDTAPRRQHGRPRVRLAYHLTPERVLGEQHASGVE
                     FSVTGTGEARRIDAGLVLTSIGYRGKPIRDLPFDESTAVVPNDRGRVVDPDSGRPVPG
                     AYVAGWIKRGPTGFIGTNKSCSFQTVQALVADFNGGKLGEPAAQPEALAKLVQARQPD
                     AVDSAGWRAIDAAEITRGRHDGRPRNKFTDVAEMLAAAATAQPAPPLRRRLLARLRDF
                     "
     gene            complement(7476..8501)
                     /locus_tag="VFI55_08245"
     CDS             complement(7476..8501)
                     /locus_tag="VFI55_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HET7074054.1"
                     /translation="MARTRMVRRWRRNMEVRDDAEYVNMLATLSEGSVRRNFNPYTDI
                     EWDSPEFAVTADDPRWILPMTDPLGRHPWYQAQSEEDKIKIGMWRQANVAKVGLHFES
                     ILIRGLMNYTFWVPNGSPEYRYCLHESVEECNHTMMFQEMVNRVGADVPGMPRMLRWT
                     SPLIPLAAGPLPVAFFIGVLAGEEPIDHTQKNVLREGKSLHPIMERVMAIHVAEEARH
                     ISFAHEFLRKRLPELTRSQRFWVSLFYPLTMRTLCQAIVVPPKAFWQEFDIPREVKKE
                     LFFRSPESRKWLSDMFADVRMLANDTGLMETRSARLMWRICKINGKPSRYRSEPQRQH
                     LAAAPAA"
     gene            8745..9863
                     /gene="serC"
                     /locus_tag="VFI55_08250"
     CDS             8745..9863
                     /gene="serC"
                     /locus_tag="VFI55_08250"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HET7074055.1"
                     /translation="MADQLEIPADIKPRDGRFGCGPSKVRPEQLTALTTTAAALFGTS
                     HRQAPVKNLVGRVRSGLTELFSVPDGYQVILGNGGATAFWDAAAFGLIDKRSLHLSFG
                     EFSSKFASAVGNNPFVGDPIVIKSDAGSAPEPQSDPSVDVIAWAHNETSTGVAVPVQR
                     PAGSDGALVAIDATSGAGGLPVDITEADVYYFSPQKNFASDGGLWLAIMSPAALARVE
                     SIAASGRWVPDFLSLPIAVENSLKDQTYNTPAIGTLALMAEQLDWLLGNGGLDWAVKR
                     TADSSRRLYSWAQEQPYTTPFVADPALRSQVVGTIDFVDDVDAAAVAKTLRANGIVDT
                     EPYRKLGRNQLRVAMFPAIDPDDVSALTQCVDWVVERL"
     gene            9986..10765
                     /gene="sepH"
                     /locus_tag="VFI55_08255"
     CDS             9986..10765
                     /gene="sepH"
                     /locus_tag="VFI55_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HET7074056.1"
                     /translation="MRELKVVALDHDGKYVICEGGEPAEKFKVAIDNRLRAALRGEPP
                     QSEQPQLDIEVTNMLSPKEIQARIRAGASVEQVAAASGSDITRIRRFAHPVLLERSRA
                     AELSTAAHPVLADGPAVLTLLETISAALVARGLEPDKLSWDAWRNEDSRWTVQLAWKA
                     GRSDNVAHFRFLPGAHGGTVTALDDAASELIDPNFERPLRPIAQVAHVDFEVGVPPAC
                     GGEAKPAVIEPPELPVHARRGKPVVPAWEDVLLGVRSGGNR"
     gene            complement(10762..>10856)
                     /locus_tag="VFI55_08260"
     CDS             complement(10762..>10856)
                     /locus_tag="VFI55_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2537 domain-containing protein"
                     /protein_id="HET7074057.1"
                     /translation="LVFRWFVLGAAAGVAGAWLTLLALTVSGTV"
BASE COUNT         1764 a         3600 c         3682 g         1810 t
ORIGIN      
        1 gacatcacca catcggagga gcacgatcgg ctgcgcgggt tggaggtcga actcgaccag
       61 tgctgggacc tgctgaggca gcgccgggcg ctgcgcgaga ccggtggcga cccacgcgag
      121 gcagaggtgc gtccgcccaa ccaggtcgag ggctacctga actgagcaag gacccagcgg
      181 acctagacgt catagtcata ggcgggggcc acaacgggct ggtcgcggcc ggttacctcg
      241 cccgggcggg ccttcgggtg cggctgctgg agcggcttgg gcacgtcggc ggggccgcgg
      301 tgtcggcgca ggccttcgat ggtgtcgagg tccggctgtc gaggtactcc tacctggtca
      361 gcttgctgcc gtcgcgcatc gtcgaagacc tgggcgcctc ggtgcggttg gccaagcggc
      421 aatattcgtc gtacacgccg gaccccggca cagccgggtg cacgggtctg ctggtcgggc
      481 gccccgacac gttcggcgcc atcggcgccg tccgcgacga gagtgggttc gccgctttct
      541 accagcgctg ccggctggtg accgagcgcc tctggccgac gttgatcgaa ccgctgcgca
      601 cccgcgagca ggcccgccgg catgtcctgg cgggcggccg tcccgacgcg gcgaccgcgt
      661 ggcgggcgat ggtcgacgag ccgatcgggc acgccatcgc cgacgcggtg agcagtgacc
      721 tggtccgcgg cgtgatcgcc acggacgcgc tgatcggcac gttcgctcgc ctcgacgacc
      781 cgtcactgcg gcagaacatc tgcttcctgt accacgtact cggcgggggt accggcgact
      841 gggacgtgcc cgtcggcggg atgggcgcgg tgaccaccgc gctagccgtc gccgctaccc
      901 gccatggtgc cgaaataacc accggcgccg acgtttacgc cgtcgaaccg gacggccggg
      961 tgcgctaccg catcggcgac gacgagcaca cggtgcgggg tcggttcgtt ctggcgggcg
     1021 tcacgccggc cgttctggcg ggtttgctcg gcgaagaacc gccgcgggcg gcccagggcg
     1081 cgcaggtcaa ggtgaacatg gtgctccgcc gactgccccg gttgcgcgac aacagcgtga
     1141 cacccgagca ggcattcgcc gggacgttcc atgtcaacga gacgtggacc caactggacg
     1201 cggcgtatac cgaggcggcc gcaggtcggc tacccgatcc ggtgccgtgc gaagcctatt
     1261 gccattcgct gaccgacccg ggcatcatgt cggccgcgct tcgtgattcc ggcgctcaca
     1321 cgatgacggt gttcggcttg cacaccccgg actcgctgtt cgccggcacc ggctccgaga
     1381 ctctgggcga gcggctcacc gatgcggtgc tggcgtcgct gaattccgtc ttggccgaac
     1441 cgatccagga cctgctgatg agcgacgcgc atggccgggc gtgcatcgag accaccacca
     1501 cccgggattt gcaccgcacc ctggggatga ccgcaggcaa catcttccac ggcggcctgt
     1561 cgtggccgtt cgccgccgac gacgacccgc tggacacccc ggcccggcaa tggggagtcg
     1621 ccaccgccca cgcgcgagtc atgctgtgcg ggtcgggtgc ccgccgcggc ggggcagtgt
     1681 cgggcatcgg cggccacaac gccgcgatgg ccctgctcgc ttcgctgtga tcgcggcccg
     1741 gcgcggtggc taccgcgtgt cgatcgcggc gtagtcgcgt tcggtgtaac cgcagtagat
     1801 ctggcgcgga cggccgatct tgctgtcgcc ctcgtcgtgc atctcacgcc agtgcgcgat
     1861 ccagcccggc agccggccca acgcgaacag cacggtgaac attcgggtcg ggaatccgag
     1921 ggcgcggtag atcagcccgg tgtagaagtc gacgttgggg tagagcttgc gctcgatgaa
     1981 gtagtcgtcg gtcagggccg tctcttcgag agccttggcg atgcccagta gggcgtcgtc
     2041 gccgcccagc ttggccagga tcttgtcggc ctgttccttg acgatccggg cccgggggtc
     2101 gtagttcttg tagacccggt gaccaaagcc catcagtttg gcgccggcct cgcggttctt
     2161 cactttccgg acaaattcgt cgacatcgtc gccgctgtcg cggatggttt ccagcatctc
     2221 gagcaccgcc tgattggcgc cgccgtgcag cgggccccac agcgcgttga tgccgccgga
     2281 gatcgaggtg aacaggttgg ccttcgacga gcccaccagc cgaaccgtcg acgtcgaaca
     2341 gttctgctcg tggtcggcgt gcaggatgaa cagcatgtcc agtgcccgca ccacctcggg
     2401 atcggccacg tacggctcgg ccggcaaccc gaatgtcatc cacaggaagt tctccaccag
     2461 cgacagcgag ttgtccgggt agaggaacgg ctgtccgacc gacttcttgt aggcgtacgc
     2521 ggcgatggtg ggcagcttgg cgagcaagcg aatggtcgac agctcgacct gaccgttgtc
     2581 caccgggtcc agcgcgtcgg ggtaataggc gctcaacgcg ttcaccacgc tggacaacac
     2641 cggcatcggg tgcgcattgc gcgggaagcc gtcgaagaaa cgcttcaggt cctcgtgcag
     2701 catggtgtgc agctgaatcc gcttggtgaa ttgggcaagc tgatccttgt ccggcagctc
     2761 gccgtagatc agcaggtagc tcacctcgat gaaggtcgac ttctcggcga gctgctcaat
     2821 cggatacccg cggtagcgca gaatgccggc atcgccgtcg atgtaggtga tggagctctt
     2881 gcacgcggcg gtgttcacga agccgttgtc gaaggtggta tgcccagttt tggccaaaag
     2941 cgaccccaac gcgatgccgt cagctccttc ggtggcatgc acgacctcga ggtcgatctc
     3001 gcccccgggg tacttcagag ttgcggtgtc gtcggtgtcg cccacgagaa cccctttgcg
     3061 cgctggtgga taatggctgc cctgactagg tctgtatagc tgaaagtagt cgttgtggcg
     3121 agggcgcgcc tgcccggggt cgggtctgct gctcacgccg ccatccctcc ggctaaatct
     3181 cgctgcattc atgcgagtcc ggctctccgc gcggtgttca acggatgccg gcgggcagcc
     3241 accgaccacg acatgagccg ggcgcgtcag ggctgcagac gctcggcttg gccgtggaag
     3301 accctgattc ggttgtgcac gcggttctcg cgcccctgcc agaactccac cacttcgggc
     3361 gccatcaggt atccgcccca gttgggcggc accgggacca gctccgagtc ggcgaagcgc
     3421 tcggtaacct cggtcagctg gtcgagcagc gccgcgcgcg acgcaatggg cgacgattgg
     3481 tgtgacgccc acgcacccag ttgtgagccg cgcggccgct tggaccagta gtcctgcgtc
     3541 acccgcgggt cgaccttggt caccgggccg cggatatgga cttgccgtcc cagctggtac
     3601 cagggaaagg tcgccgacgc gtagggcatc gccgccagct caatgccctt ggccgagtca
     3661 tagttggtga aaaaactgat cccggtctcg tcggcgcttt tgcacaagac cgaacgactg
     3721 accggcttgc cctccgcgac ggtggccaac accatggcgt tgggctcggc caccccggcg
     3781 cgttcggcgt cctcgagcca ctttcggaac aacgcaagcc agccctccgc caaccaatcc
     3841 acgtccaggt cggggctgcc gtccttctcg acggaaccgt attccacgcg catccgctgc
     3901 aggtgctgct cgtgtggtcc tgccattgcg ccaacgctac ccgcgaggct gcttccggcc
     3961 ggtagcgtcg gcgcggacgt ggggtgcgag aattttgccc atgactgtgg tccccgagaa
     4021 ctttgtcccc ggcctagaag gcgtggtggc gttcaccacc gaaattgccg aaccggataa
     4081 agacggcggc gcgctgcgct accgcggcgt cgacatccaa gatctggtga atcagcaggt
     4141 cacgttcggt gacgtgtggg cgctgctggt cgacggcaaa ttcggtcacg ggctgccgcc
     4201 cgcggagcca ttcccgctgc cgatccatac cggcgacgtg cgggtcgacg tgcaggccgg
     4261 actggcgatg ctggcgccga tctgggggta caaaccgctg ctcgacaccg acgaatccac
     4321 cgcgcgcgat cagctggcac gggcatcggt gatggccctt tcctatgtcg cccaatccgc
     4381 gcgcggcatc tatcagccgg ccgtcccgca gcgagtcatc gatgaatgcc cgaccgttac
     4441 ggcgcgattc atgacccgct ggcaaggcga tccggacccg aggcatatag aagccatcga
     4501 tgcctactgg gtgtcggccg cggagcacgg gctgaacgcg tcgacgttca ccgcgcgggt
     4561 gatcgcctcg acgggcgcgg acgtggcggc cgcgctgtcc ggggcgatcg gggcgatgag
     4621 tggcccactg catggcgggg cgccggcgcg cgtgctgccg atgatcgagg aggtcgagcg
     4681 caccggcgac gcccgcgccc tggtcaagtc ggtcctcgac agcggcgaca agctgatggg
     4741 attcgggcac cgcgtctacc gcgcggagga tccccgggcg cgcgtgctgc gggcgaccgc
     4801 ggaacggctg ggcgcgccgc gctacgaggt agcggtcgcg ctggagcagg ctgcgctggc
     4861 cgagctccgg gagcggcgtc cggaccgggc catcgagacc aacgtcgagt tctgggccgc
     4921 ggtgatcctg gacttcgcgc gggtaccggc caacatgatg ccggcgatgt tcacctgtgg
     4981 acgcaccgcg gggtggtgcg cccacattct cgagcagaag cggctgggca agctggtccg
     5041 cccgtcggcc atctatgtgg gaccggagcc gcgcagcccg gaatcggtcg agggttggga
     5101 tcgggttctc acttctgcgt gagaggcctg agtcgggttt gttgtcggtg cccggcggtt
     5161 aaatgattct cggctgcgat gaaccgatga gttcgcatcg cggacggagt cattaaccgt
     5221 gagccggcgc ccgcctgggg ccggcacccg agaccgacag caaggagacc accatggcta
     5281 tcaccatcga acccacactg tccccacacc tcgtggtcga cgacgccgcc gcggcgatcg
     5341 acttctacgt caaggctttt gacgccgtcg agctgggtcg ggtgcccggt cccgacggca
     5401 ggctgatcca cgcggcactg cggatcaacg gtttcacggt gatgctcaac gacgattttc
     5461 ccgagacgtg cggcggcaag tcgatgacgc ccaagtcgct gggtggaacc ccggtcacca
     5521 ttcaccagac cgtgaccgac gtcgagacca agttccaaca ggcgctggac gccggcgcca
     5581 ccgtggtgct cccgttggaa gaccagttct ggggagaccg ctacggcatg gtcgccgacc
     5641 cgttcggcca ccactggtcg ctgggacagc cggtgcgcga ggtcagcgcg gacgagatcg
     5701 cagcggcgat gtccgggcag acaagcggct agccaccctc tggtcggccc tggctagaag
     5761 tcgcgcagcc gggccagcag ccggcgtcgc agcggcggcg ccggctgtgc ggtggccgcc
     5821 gccgcgagca tctcggcgac gtcggtgaac ttgttgcgcg ggcgtccgtc gtggcggccg
     5881 cgggtgattt ctgcggcgtc aatcgctcgc caacccgcgg aatcgacggc gtcgggctgg
     5941 cgcgcctgca ccagcttggc cagcgcctct ggctgggccg ccggctcgcc cagcttgccg
     6001 ccgttgaaat cggccaccag ggcctgcacg gtttggaacg agcaggactt gttggtaccg
     6061 atgaaccccg tcggtccgcg cttgatccag cccgcgacgt atgcaccggg caccggtcgg
     6121 ccagagtcgg gatcgacgac gcgaccgcga tcgttgggca ccactgccgt cgattcgtcg
     6181 aacggaagat cgcgaatcgg cttgccgcgg tatccgatcg acgtcagcac caggcccgca
     6241 tctatccggc gcgcttcgcc ggttcccgtg accgagaact cgacgccgct ggcatgctgc
     6301 tcacccagca cgcgttctgg cgtgaggtgg taggccagcc ggactcgcgg gcgcccgtgt
     6361 tgccggcgcg gcgccgtatc gttgcacagt ttgctcagga tctcgagctt gttttcggtc
     6421 agcgggtcgg acaccatccc gagatcgttg gcaacccgca cctggtcggc ggcatcgagt
     6481 accacctcgg atgcgcccgt gagcccgacc agttcgggca gggtgaacgc cgaatgggcg
     6541 ggcccgcgcc gggcggcgat caccacttcg cgcaccatcg aacggcgaaa tgcttcgagc
     6601 gcatggtcgg agatgtcggt gcggaccagg tcgtcgggat cggcggtgag cacacgggcc
     6661 acatccaggg cgacgttgcc gttgccgatg atcaccgcgc gctcgtgact gagatcgacc
     6721 ggcagatcgg tgaagtcggg atgcccgttg atccaagcga ccagctcggt ggcggttccg
     6781 gtgcccggca gacccatccc gtcgatgtcc agtcgacgat catcgggtgc gccgaccgca
     6841 tagagcaccg catgatggtg ggccagcagc tcggcgtggc tcaggtgctt gccgatctcg
     6901 acgttgagat agaaccggaa gcgacgatgg cgcgataccc ggtcgaagag ccgggtgacc
     6961 cccttcgtgt tctggtgatc gggcgcgacg ccggcacgca ccaatccata cggcgtgggc
     7021 agcttctcga agacgttgac ccgcaccccg tgctgggtga gcaattcgtc ggcggcatac
     7081 atagccgcgg gcccggaccc cacaatggcc accgtcagcg gctgccggag ggcacgcacc
     7141 tcggcggccg ggatcaccgg ggccagcttc gacgtcggcg gcagcttcac gtccgcgggg
     7201 cgctctggat aaaaggaggc gttgatctcg atgaacggca gctgtttggg gtccagccgg
     7261 ctgtccggcg cgatcgcacc gaccgggcag gcgttcacgc acgcgccaca gtcgacgcag
     7321 gccaccggat cgatgtacag catctccgac gtcgcgaagc ccggctcatc cggcgtcggg
     7381 tgaatgcaat tcaccgggca cgcgaagacg caggacccgt cgttgcaaca cgactgggta
     7441 ataacgtgcg gcataagtac cgaacccgcg cggtcctacg cggccggtgc cgcggccagg
     7501 tgctggcgtt gcggctcgct gcgatagcgc gacggcttgc cgttgatctt gcagatccgc
     7561 cacatcaggc gcgccgagcg cgtctccatc aggccggtgt cgttggccag catccgcacg
     7621 tcggcgaaca tgtcgctcaa ccactttctc gactccggcg accggaagaa gagttccttc
     7681 ttgacctcgc gcgggatgtc gaactcctgc cagaacgcct tgggcggtac cacgatcgcc
     7741 tggcacagcg tcctcatcgt cagcgggtag aacagcgaga cccagaaccg ctgactccgg
     7801 gtcagctcgg gcagccgctt ccgcaggaac tcgtgggcaa aggagatgtg ccgggcttcc
     7861 tcggccacgt gaatggccat cacccgctcc atgatcgggt gtaacgactt gccttcgcgg
     7921 agcacgtttt tctgggtgtg gtcgatgggt tcctcgccgg cgagcacccc gatgaaaaac
     7981 gcgaccggca acgggcccgc cgccagcgga atgagcggcg aggtccagcg cagcatgcgc
     8041 ggcatccccg ggacatcggc cccgacacgg ttgaccatct cctgaaacat catcgtgtgg
     8101 ttgcactctt cgacggattc gtgcaggcag taccggtatt ccggcgatcc gttgggcacc
     8161 cagaaggtgt agttcatcag gccgcgaatc aggatcgatt cgaagtgcag gccgaccttg
     8221 gccacgttgg cctgccgcca catgccgatc ttgatcttgt cttcttccga ctgggcttga
     8281 taccagggat gacgccccaa cgggtcggtc atcggcagga tccaccgtgg atcgtccgcg
     8341 gtgaccgcga attccggtga atcccactcg atgtcggtgt agggattgaa gtttcgccgc
     8401 accgacccct cagacagtgt ggcaagcata ttcacgtatt cggcgtcgtc gcgcacctcc
     8461 atgttgcggc gccaacgtcg aaccattcgc gtcctagcca tatccaagcc tccccagcga
     8521 ggtttacatt tggacgttct gtgtctagac agtaccgcag gtaccggata ttttccagac
     8581 ctcgttgaag ccctggggcc tgaccagcgg tcaagcgcgg ggtgtgtcat agctcacatc
     8641 tccagcgcgg cggcaggcgg tcgtcctggc gttatccgtc gccgcaacgc gcacaatcgg
     8701 cccgcgacga cggcgccccg ggagcgctca ctaggctggt cgacatggct gaccagctcg
     8761 agatccccgc tgacatcaaa ccccgcgacg gccgcttcgg ctgcggcccc tcaaaggtcc
     8821 gacccgagca gttgacggcg ttgaccacta ccgcggccgc ccttttcggc acatcgcacc
     8881 ggcaagcacc cgtcaagaat ctggtgggcc gggttcggtc gggattgacc gaactcttct
     8941 ccgtgccgga cggttaccag gtcattctgg gcaacggtgg cgcgacggcg ttctgggatg
     9001 ccgcggcttt cgggctgatc gacaagcgct cgctgcacct ttccttcggc gagttcagct
     9061 ccaagttcgc ctccgcggtc ggcaacaacc cgttcgtcgg cgatcccatc gtgatcaagt
     9121 cggacgccgg cagtgcgccg gagccgcagt ccgacccgtc ggtcgacgtg atcgcctggg
     9181 cgcacaacga gacgtcgacc ggggtcgcgg ttccggtcca gcgccccgcc ggctcggacg
     9241 gcgccctggt cgccatcgac gcgacctccg gcgccggcgg cctaccggtc gacatcaccg
     9301 aggccgacgt gtattacttc tcgccccaga agaacttcgc cagcgacgga gggttgtggc
     9361 tggccatcat gagccctgcc gcgctggccc gcgtcgagtc catcgccgcg tccggccgct
     9421 gggtgcccga cttcttgtcg ctgccgatcg cggtcgagaa cagcctgaag gaccagacct
     9481 acaacactcc ggcgatcggc accctggcgc tgatggccga acagctcgac tggctgctgg
     9541 gcaacggcgg gctggactgg gcggtcaagc ggacggcgga ctcgtcgcgg cggttgtact
     9601 cctgggcgca ggagcagccc tacaccacgc cgttcgtcgc cgacccggcg ttgcggtccc
     9661 aggtggtggg cacgatcgac ttcgtcgacg acgtcgacgc tgcggccgtc gcgaagaccc
     9721 ttcgggctaa cggcatcgtc gacaccgagc cgtaccgaaa gctcggccgc aaccagttga
     9781 gggtcgcgat gttccccgcg atcgaccccg atgacgtcag cgcactcacc cagtgcgtcg
     9841 actgggtggt cgagcggctt taaccgggtg gtcatgagcc ggcaacccgc cagcgtgtca
     9901 gagcgggttg ccggcgtcgg ctgcactaga gtgcgcacct gacgggtcgg tcggtgaccg
     9961 ggcacggagc ctgcgaggag aagccatgcg ggaactcaaa gtggtggcgc tcgatcacga
    10021 cggcaaatac gtcatctgcg agggcggtga gcccgcagag aagttcaagg tggcgatcga
    10081 caaccgactg cgggccgcgc tgcgcggcga accgcctcag tcggagcaac cccagctcga
    10141 catcgaagta acaaacatgc tgagccctaa agaaattcag gccagaatcc gtgccggagc
    10201 gtccgtcgag caggtggctg cggcgtcggg ttccgatatc acgcggatcc gccggttcgc
    10261 ccacccggtg ttgctggagc gttctcgcgc cgcggaactg tcaacggccg cgcatccggt
    10321 gctcgccgat ggcccggcgg tgctgacgct gttggagacc atcagcgccg cattggtggc
    10381 gcgcggcctg gaacccgaca aactcagctg ggacgcctgg cgcaacgaag acagccgctg
    10441 gacggtgcag ctggcgtgga aggcgggacg ttccgacaac gtcgcgcact tccgcttctt
    10501 gccaggcgcc catggcggca cggtcaccgc gctcgacgac gcggcgagcg agttgatcga
    10561 cccgaacttc gagcgaccgt tgcgaccgat cgctcaggtg gcccacgtgg acttcgaggt
    10621 gggggtaccg cccgcttgcg ggggagaggc gaagcccgcg gtcatcgagc cgcccgagct
    10681 gccggtgcac gcccgacgcg gcaaaccggt cgttccggct tgggaggatg tgctgctcgg
    10741 cgtgcgctcg ggcgggaacc gttagacagt tcccgacacc gtcagcgcga gcagcgtcag
    10801 ccacgcgccc gccacgcccg cggccgcacc cagtacaaac cagcgaaaca ccaaga
//
LOCUS       DASSGQ010000187         8428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_469131,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000187 DASSGQ010000000
VERSION     DASSGQ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8428
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469131"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2305
                     /locus_tag="VFI55_08265"
     CDS             <1..2305
                     /locus_tag="VFI55_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET7074058.1"
                     /translation="GAARSIGRARDIEPRHRRDGIALVLLGLAVVVAASSWFDAARPV
                     GAWVDVLLRTFIGSAVLVLPLVLAGVAVVLMRTQPNPEARPRLILGASLIALPVLGLR
                     HLWSGSPEDPELRRRAGGFLGFAIGGPLSDGLTPWIAAPLLFIGAVFGLLLLTGTAIR
                     EVPEAVRAMFGTRLFQREYGDQDGPEYDDTDEFTDDADAPAREDFSDGYYDDASSSPE
                     NEPQAWPSADSSQLPLEDDIPTIPEPAVTRSRRRGRSAAKEDTHELDRVVEGPYTLPS
                     LSLLVAGDPPKKRSAANTVMSDAISEVLNQFKVDAAVTGCTRGPTVTRYEVELGPGVK
                     VEKITALQKNIAYAVATESVRMLAPIPGKSAVGIEVPNTDREMVRLADVLTASSTRRD
                     HHPLVIGLGKDIEGDFISANLAKMPHLLVAGSTGSGKSSFVNSMLVSLLTRATPEEVR
                     MILIDPKMVELTPYEGIPHLITPIITQPKKAAAALTWLVDEMEQRYQDMQASRVRHID
                     DFNAKVRSGAITAPLGSQREYRPYPYVVAIVDELADLMMTAPRDVEDAIVRITQKARA
                     AGIHLVLATQRPSVDVVTGLIKTNVPSRLAFATSSLIDSRVILDQAGAEKLIGMGDGL
                     FLPMGASKPVRLQGAYITDEEIQAVVAACKDQAEPEYTEGVTTAKPTGERTDVDPDIG
                     DDMDVFLQAVELVVSSQFGSTSMLQRKLRVGFAKAGRLMDLMETRGIVGPSEGSKARE
                     VLVKPDELAGTLALIRGGAGADGTDAE"
     gene            complement(2330..2854)
                     /locus_tag="VFI55_08270"
     CDS             complement(2330..2854)
                     /locus_tag="VFI55_08270"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HET7074059.1"
                     /translation="MTESSRDYRPVVRRARTSDVAAIKQLVDTYAGRILLEKNLVTLY
                     EAVQEFWVAEHPDIPGRVIGCGALHVLWSDLGEVRTVAVDPAVTGRGIGHAIVNQLLD
                     VARELQLKRLFVLTFETEFFGKHGFTEIEGTPVTAEVYEEMCRSYDIGVAEFLDLSYI
                     KPNILGNSRMLLVL"
     gene            2938..3501
                     /gene="pgsA"
                     /locus_tag="VFI55_08275"
     CDS             2938..3501
                     /gene="pgsA"
                     /locus_tag="VFI55_08275"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET7074060.1"
                     /translation="MAGRARIANLANILTLLRLVLVPIFLLALFAGGGHQIAGRIVAF
                     VIFAIACITDRFDGLLARNYGMATEFGAFVDPIADKTLIGSALIGLSMLGDLPWWVTA
                     VILTREVVVTLLRLAVIRRGVIPASWGGKVKTVVQALAIGLFVLPHSGPFLVVASVVM
                     GAAILLTVVTGIDYVVSTVRELRRTSD"
     gene            3601..3939
                     /gene="clgR"
                     /locus_tag="VFI55_08280"
     CDS             3601..3939
                     /gene="clgR"
                     /locus_tag="VFI55_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ClgR"
                     /protein_id="HET7074061.1"
                     /translation="MPPLVREVIGDVLRLARTSQGRTLREVSDVARVSLGYLSEVERG
                     RKEASSELLHAICDALDVPLSSVLTDAGERMAGEELAAHSAPTSAASTTRIDAGTKVV
                     IPPVASLAVA"
     gene            4096..4926
                     /gene="pspA"
                     /locus_tag="VFI55_08285"
     CDS             4096..4926
                     /gene="pspA"
                     /locus_tag="VFI55_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HET7074062.1"
                     /translation="MANPFVKAWKYVMALFNAKIDEHADPKIQIQQAIEEAQRTHQAL
                     TQQAAQVIGNQRQLEMRLNRQLADIEKLQVNVRQALTLADQATAAGDTAKAAEYNNAA
                     EAFAAQLVTAEQSVEDLKTLHDQALSAAAQAKRAVEQNSMVLQQKIAERTKLLSQLEQ
                     AKMQEQVSASLRSMSEIAAPGNTPSLDEVRDKIERRYANAIGSAELAQSSVQGRMLEV
                     KKAGIEMAGHSRLEQIRASMRGEALPTGGTPAAPGAIPASPKPAESTGVPVAEKPFGQ
                     "
     gene            4945..5763
                     /locus_tag="VFI55_08290"
     CDS             4945..5763
                     /locus_tag="VFI55_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074063.1"
                     /translation="MAVKATESGPWRTLLQRGLNAAADLSDLVAQKISAATDPRARLL
                     RRRRRALRWGLIFTAGCLFWVVVTGVLAAWGWFALLLEITGGVAVVMAIPATLLLLRY
                     RWLRSEPLPARRSVDARRLPPAGSAARPAMSALGASERGFFSLLGVIERGSMLPQAEI
                     RDLTAAANRASASMAATATEVASMERAAYYSESSRPYLVPTINAFTAQLSAGVRQYNE
                     MVTAAAQLVSSANGDTGIALATSQQRYRAELVEATDRLIGWAQAFDELGGLPRR"
     gene            complement(5773..6210)
                     /locus_tag="VFI55_08295"
     CDS             complement(5773..6210)
                     /locus_tag="VFI55_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5313 domain-containing protein"
                     /protein_id="HET7074064.1"
                     /translation="MSDTARTKPNALQFVRYCAGGRLPDSMREWVRNDLAGKGATRRM
                     MVRVAVPAVLVLAPFWLIPAALDVHLSMTLPILIPFVYFSHALNKVWRRHMLAVHDLN
                     PDLVDELDRERDAHIHQAYIERYGPRTESGPPQNSPPGHGADG"
     gene            complement(6238..6669)
                     /locus_tag="VFI55_08300"
     CDS             complement(6238..6669)
                     /locus_tag="VFI55_08300"
                     /EC_number="3.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007172550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018744 - limonene-1,2-epoxide hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase"
                     /protein_id="HET7074065.1"
                     /translation="MTELTGTAVANTRTVEGFLNALQDQDFEAADAALDDNLIYENVG
                     LPTIRGRARAMNLFRRMEGRAAFEVKIHRIAGDGAAVLTERTDALIFGPLRLQFWICG
                     VFEVHNGKITLWRDYFDFFDMFKATARGVAALVVPSLRASF"
     gene            6723..7889
                     /locus_tag="VFI55_08305"
     CDS             6723..7889
                     /locus_tag="VFI55_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7074066.1"
                     /translation="MRIAVVAGPDPGHSFPAIALCQRFAEAGDEPTLFTGSEWIEAAG
                     GAGIDAVPLDGLVATDEDLDAGARIHRRAARMAVLNVPALRELAPDLVVSDVITAGGG
                     MAAELLGIPWIELNPHPLYLPSKGLPPIGSGLAPGIGVRGRLRDVTMRSLTARSWRAG
                     LKQRAAVRLQIGLPAVDPGPLRRLIATLPALEVPRPDWPEEAVVVGPLHFEPTDRALE
                     IPPGPGPVVVVAPSTALTGTSGLAEVALECLIPGQTLPVGARLVVSRLGGADLAAPPW
                     AVVGLGRQSELLTHADLVICGGGHGMVAKSLLAGVPLVVVPGGGDQWEMANRVVRQGS
                     ARLIRPLSADALVAAVDEVLSAPGYRGAAERAAGSIAGVADPVRVCHEALALAG"
     gene            7901..8107
                     /locus_tag="VFI55_08310"
     CDS             7901..8107
                     /locus_tag="VFI55_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3046 domain-containing protein"
                     /protein_id="HET7074067.1"
                     /translation="MLADVRLTEFNERVVLRFGAAYGASVLVDHVLTGLDGRTAARAI
                     EDGVEPRDVWRALCVDFDVPRDQW"
     gene            complement(8250..>8428)
                     /locus_tag="VFI55_08315"
     CDS             complement(8250..>8428)
                     /locus_tag="VFI55_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7074068.1"
                     /translation="IVAITNSLLDTGTLAGSGLITTPHLGFRRRSCCLFYRVPGRSVC
                     GDCVFRRTPRPNRS"
BASE COUNT         1344 a         2751 c         2979 g         1354 t
ORIGIN      
        1 gggggcggcc cggtcgatcg ggcgggcccg cgacatcgag ccccgacatc gccgcgacgg
       61 aatcgcgctg gttctgctcg gtcttgccgt ggtggtcgcc gcgagctcgt ggttcgacgc
      121 ggcccggccg gtcggggcgt gggtcgacgt tttgctgcgg acattcatcg gctcggccgt
      181 gctggtgctg ccgctggtcc tggccggggt ggcggtggtg ctgatgcgga cccagcccaa
      241 cccggaagcg cggcccaggc tgatcctcgg cgcgagcttg atcgccctgc cggtcctcgg
      301 cctgcgccac ctgtggtccg gttccccgga agacccggaa ttgcggcgtc gcgccggagg
      361 attcctgggc ttcgccatcg gcgggccgtt gtcggacggg ttgacgccat ggatcgccgc
      421 gccgctgttg ttcatcggcg cggtgttcgg gctgctgttg ctgaccggga ccgcgatccg
      481 cgaggtgccc gaagcggtgc gtgccatgtt cggcacccgg ttgttccagc gtgaatacgg
      541 cgaccaggac gggcctgagt acgacgacac ggacgagttc accgatgacg cggacgcccc
      601 tgcgcgcgaa gacttttccg acggttacta cgacgacgcg tccagtagcc cggaaaacga
      661 gccgcaggcg tggccgtcgg ccgactcttc tcagctcccc ctcgaggacg acatccccac
      721 cattcccgaa cctgccgtca cccggagccg ccggcgcggc cggtccgctg cgaaagagga
      781 cacccacgag ctggaccggg tcgtcgaggg tccctacacg ctgccgtcgc taagcctgct
      841 ggttgccggc gatccaccca agaaacgcag cgccgccaac actgtgatgt ccgacgcgat
      901 cagcgaggtg ctcaaccagt tcaaggtcga cgcggccgtc accggctgca cccgcgggcc
      961 gaccgtcacc cgctacgagg tcgagctggg gcccggcgtc aaggtggaga agatcaccgc
     1021 gctgcagaag aacatcgcgt atgcggtggc caccgaaagc gtccgcatgc tggccccgat
     1081 ccccggcaag tccgccgtcg gcatcgaggt gcccaacacc gaccgggaaa tggtgcggct
     1141 ggccgacgtg ctgaccgcgt cgtcgacccg ccgcgaccac cacccgttgg tgatcgggtt
     1201 gggcaaagac atcgaagggg atttcatctc ggccaacctg gccaagatgc cgcacctgct
     1261 ggtcgccggc tccaccggat ccggcaagtc cagcttcgtc aactcgatgc tggtgtcgct
     1321 gctgacccgg gccaccccgg aagaggtcag gatgatcctg atcgacccga agatggtgga
     1381 actgacgccc tatgaaggca ttccgcatct gatcaccccg atcatcaccc agccgaagaa
     1441 ggcggccgcg gccctgacgt ggctggtcga cgagatggaa cagcgctacc aggacatgca
     1501 ggcttcccgg gtgcgtcaca tcgacgactt caacgccaag gtgcgatccg gtgccatcac
     1561 ggcgccgctg ggcagccagc gggagtaccg gccctacccg tacgtggtgg cgatcgtcga
     1621 cgagctggcc gacctgatga tgaccgcgcc gcgcgacgtc gaggatgcga tcgtgcggat
     1681 cacccagaag gcccgcgccg cgggcatcca cctggtgctg gccacccagc gcccgtcggt
     1741 ggacgtcgtg accgggctga tcaagaccaa cgtgccgtcc cggctggcgt tcgcgacgtc
     1801 gtcgctcatc gacagccggg tgatcctgga ccaggccggg gccgaaaagc tgatcggcat
     1861 gggcgacggc ctgttcctgc cgatgggtgc gagcaagccg gttcggttgc agggcgccta
     1921 catcaccgac gaggagattc aggctgtcgt cgcggcgtgt aaggaccagg ccgaacccga
     1981 atacaccgag ggtgtcacca ccgccaagcc caccggggaa cgcaccgacg tcgaccctga
     2041 catcggcgac gacatggatg tgttcctgca ggccgtggag ctggtggtgt ccagccaatt
     2101 cgggtccacc tcgatgctgc agcgcaagct gcgggtgggc ttcgccaagg ccggccggct
     2161 gatggatctg atggagaccc gcggcatcgt cgggcccagc gagggctcca aggcgcgcga
     2221 ggtgctggtc aagcccgacg agctggccgg aacgctggca ctgatccgcg gcggggccgg
     2281 cgccgacggt acggacgccg agtagtgccg aacgtgcact aagggcgact cacaaaacca
     2341 gcaacatccg ggagttgccc aggatgttcg gcttgatgta gctcagatcg aggaactcgg
     2401 caacgccgat gtcgtaggag cggcacatct cctcgtatac ctcggccgtg acgggcgtgc
     2461 cctcgatctc ggtgaacccg tgcttgccga agaactccgt ctcgaaggtc aacacgaaca
     2521 gccgcttcaa ctgcagctcg cgggccacgt ccagcagctg attgacgatc gcgtggccga
     2581 taccgcggcc ggtcacggcc gggtcgaccg cgacggtacg aacctcgccg agatcggacc
     2641 acaacacatg cagcgccccg caaccgatca ccctgcctgg aatatccggg tgttcggcca
     2701 cccagaactc ctggacggcc tcgtacagcg tgacgaggtt cttctccagc aggattcggc
     2761 cggcatacgt gtcgacaagt tgcttgatcg ccgcgacatc cgaggttcgc gcacgccgca
     2821 ccactggccg gtaatcccgt gaactttcgg tcacgggtaa cagtatggtc agccggggtg
     2881 tgctcgctgg tcaacggtta ttctgttgcg gtgtcggggc agccgcagac gagtccgatg
     2941 gcgggccgcg cccgcatcgc caacctggcc aatatcctga ccctgctgcg actggtgctg
     3001 gtcccgattt tcctgctcgc cttgttcgcc ggcggcggcc accaaatcgc cggtcgcatc
     3061 gtagctttcg tgatttttgc gatcgcatgc atcaccgacc gcttcgacgg cctgctggcc
     3121 cgaaactacg ggatggcgac cgaattcggc gcgttcgtcg acccgatcgc cgacaagacc
     3181 ctgatcgggt cggcgctgat cggcctgtcg atgctgggcg acctgccgtg gtgggtcaca
     3241 gcggtgatcc tgacccgcga ggtggtggtc acactgctgc ggttggcggt gattcgccgc
     3301 ggggtgattc cggcaagttg gggcggcaaa gtcaagaccg tggttcaggc gctggcgatc
     3361 ggcctgttcg tgctgccgca ttcgggaccg ttcctggtgg tggcgtccgt ggtgatgggc
     3421 gccgcgatcc tgctcaccgt ggtcacgggt atcgactacg tggtgtcgac cgtccgggaa
     3481 ctgcgccgga cgagcgactg accgggaacc aacgcccacg ctgccggcgt tcacctagtg
     3541 agtgtctgct gaatggggtt gttcggctgc tgagcaggcg tgactggacg gaggagcagg
     3601 atgccgccat tggtgcgaga ggtcatcggc gacgtgttgc gcctggcccg gacgtcgcag
     3661 ggccggacgc tgcgggaggt gtcggatgtg gcgcgcgtga gcctcggcta cttgtcggag
     3721 gtggagcgcg gccgcaagga ggcgtccagc gagctgctcc acgcgatctg cgacgcgttg
     3781 gacgtgccgt tgtcgtcggt cctcaccgac gccggcgagc gaatggcggg cgaggaactc
     3841 gccgcccaca gcgcgccgac cagcgcggca agcacaacca gaatcgacgc cggcaccaag
     3901 gtcgtcatcc caccggtggc gtcgctggcc gtggcctgag tgccccgggg tcgcgcggct
     3961 cgatcaggat ccaccgcact ggggtggggc gatcggcgtt gacccactaa attgggcgac
     4021 aaggtgagcg cagagccaat ccaggggccc gtccaaggcc caccggcaca caagacccga
     4081 aggtggagct aacccatggc caatccgttc gtcaaagcct ggaaatacgt catggcgctg
     4141 ttcaacgcga aaattgacga gcacgccgac cccaagatcc agatccagca agccatcgaa
     4201 gaagcccagc ggacgcacca ggcgctgacc cagcaggcgg cgcaagtgat cggcaaccag
     4261 cgccaactgg agatgcggct caaccggcag ctggcggaca tcgaaaagct gcaggtcaac
     4321 gtgcgtcagg cgctgacgct ggctgaccag gccaccgccg ccggtgacac cgccaaggcc
     4381 gccgagtaca acaacgccgc cgaggcgttc gcggcccagc tggtgaccgc cgagcagagc
     4441 gtggaagacc tcaagacgct gcacgaccag gcgctctcgg ccgcggctca ggccaaacgg
     4501 gccgtcgaac agaactcgat ggtgctgcag cagaagatcg cggagcgcac caagctgctc
     4561 agccagctcg agcaggccaa gatgcaggag caggtcagcg catcgctgcg ctcgatgagc
     4621 gagatcgccg ccccgggcaa taccccgagc ctcgacgagg tacgcgacaa gatcgaacgg
     4681 cgctacgcca acgcaattgg ttctgccgaa ctcgcccaga gttccgtgca gggccgcatg
     4741 ctcgaggtta agaaggccgg tatcgagatg gccggtcatt cccggctgga gcagatccgc
     4801 gcgtcgatgc gtggcgaagc cttgccgacc ggcgggaccc ctgcggcccc cggggcgatc
     4861 ccggcctcgc ccaaacccgc ggaaagcacc ggtgtaccgg ttgccgagaa gccgttcggt
     4921 cagtagtcgc cgcggcggtc tgacatggcg gtgaaagcga ccgagagcgg gccatggcgc
     4981 acgctgctgc agcgtgggct gaacgctgcc gccgatcttt ccgacctggt ggcccagaag
     5041 atcagcgccg ccaccgatcc gcgcgcgcgg ctgctgcgcc gccgccgccg ggcattgcgg
     5101 tggggcctca tcttcaccgc cggatgcctg ttctgggtgg tggtgaccgg tgtgctggcc
     5161 gcgtggggct ggttcgcgtt gctgctggag atcaccggcg gcgtggccgt cgtcatggca
     5221 atcccggcga cgttgctgtt gcttcgctac cggtggctgc gatccgagcc gctgccggca
     5281 cgccggtccg tcgacgctcg tcggctgccg ccggccggtt cggcagcgcg cccggcgatg
     5341 tccgcgctgg gcgcctccga acgcggattc ttctcgctgt tgggggtcat cgagcggggc
     5401 tccatgttgc cgcaggccga aatacgggac ctgaccgccg cggccaacag agcctcggcg
     5461 tcgatggcgg ctaccgccac cgaggtggcg tcgatggagc gggcggcgta ctactcggaa
     5521 tcgtcgcggc cgtatctggt gcccacgatc aacgcgttca ccgcgcaatt gagtgccggc
     5581 gttcgtcagt acaacgaaat ggtcaccgcc gcggcacagt tggtgtcctc ggccaacggc
     5641 gacacgggga ttgcgttggc gacgtcgcag cagcgttacc gcgccgagct ggtggaggcc
     5701 accgatcggc tgatcgggtg ggcgcaggcg ttcgacgaac tcggcggatt gccgcgacga
     5761 tagccggggc gctcagccgt cggcgccgtg gccgggcggc gagttttggg gcgggcccga
     5821 ttccgtgcgc gggccgtagc gttcgatgta cgcctgatga atgtgtgcgt cgcgctcgcg
     5881 gtcgagttcg tcgacgaggt ccgggttcag gtcgtgcacg gccagcatgt gacgccgcca
     5941 caccttgttg agcgcgtgcg agaagtagac gaacggaatc aggatcggca gcgtcatgct
     6001 caggtggacg tccagcgccg ccgggatgag ccagaacggg gccagcacca gcaccgccgg
     6061 aaccgcgacg cgcaccatca tccggcgcgt ggcgcccttg ccggccaggt cgttgcgcac
     6121 ccactcgcgc atcgaatccg gcagccgtcc accggcgcaa tagcggacga actgcaatgc
     6181 attcggtttg gtgcgcgccg tgtcgctcat catcgtcgcc ctccgtctcg tggcgggcta
     6241 aaacgaagcc ctgagcgagg gcaccaccag cgccgccacg cctcgtgcgg tcgccttgaa
     6301 catgtcgaag aagtcgaagt agtcccgcca cagcgtgatt tttccgttgt ggacctcgaa
     6361 cacgccgcag atccagaact gcagccgcag cgggccgaag atcagcgcgt cggtgcgttc
     6421 ggtgagcact gcggcgccgt cgccggcgat gcggtggatc tttacctcga aggccgcgcg
     6481 gccctccatc cggcggaaca ggttcatggc tctggcgcgc ccgcgaatcg tcggcaaacc
     6541 gacgttctcg tagatcagat tgtcgtccaa agcggcatcg gcggcttcga aatcctggtc
     6601 ctgcagcgcg ttcagaaaac cttcgaccgt gcgggtgttc gcaacggctg taccagtcag
     6661 ctcggtcatg attgccagcg taggccagcg tggcgacacg acgctgtggc agggtaaggc
     6721 gggtgcgcat cgccgtggtc gccgggccgg atcccgggca ctcgtttccc gcgatcgcgc
     6781 tgtgccaacg cttcgccgag gcgggcgacg agcccacgct gttcaccggc agcgaatgga
     6841 tcgaggctgc cggcggtgcg ggcatcgacg ctgtcccgtt ggacgggctg gtggccacgg
     6901 acgaggacct cgatgccggg gccagaatcc accggcgggc ggcgcggatg gccgtgctca
     6961 acgtgcccgc gctgcgcgag ctggcacccg acctggtggt gtccgacgtc atcaccgccg
     7021 gtggcgggat ggccgccgag ctgctgggaa tcccgtggat cgagctcaac ccgcatccgc
     7081 tgtacctgcc gtccaagggg ttgccgccga taggcagtgg tctggcgccg ggcatcggcg
     7141 tccgcggccg gctgcgggac gtcaccatgc ggtcgctgac ggcgcggtcc tggcgagccg
     7201 ggctgaagca gcgcgcggcc gtccggttgc agatcgggct gccggccgtc gaccccggac
     7261 cgctgcggcg cttgatcgcc accctgcccg ctttggaagt cccgcgtccc gactggcctg
     7321 aggaggccgt cgtggtgggc ccgttgcact tcgagccgac cgaccgggcg ctggagattc
     7381 cgcccggccc gggaccggtg gtggtggtcg cgccctcgac cgcgttgacc gggacctcag
     7441 ggttggccga agtcgcgctg gagtgcctga tacccgggca gacgctgccg gtgggtgcgc
     7501 gcctggtggt gtcccgcctg ggtggagcgg atctggcggc gccgccgtgg gcggtggtgg
     7561 ggctgggacg tcaatccgag ttgctgacgc acgccgacct ggtgatctgc ggcggtggtc
     7621 atgggatggt ggccaagtcg ctgctggccg gggtgccgct ggtggtcgtt cccggcggcg
     7681 gggatcagtg ggagatggcc aaccgggtgg tgcggcaggg gagtgcgagg ctgattcgcc
     7741 cgctgagcgc cgacgcgctg gtggccgcgg ttgacgaggt gctctctgcg cccggctacc
     7801 ggggggccgc cgaacgggcg gccggcagca tcgccggcgt cgccgacccg gtgcgggtgt
     7861 gtcacgaagc gctggctctc gcggggtgat gacgaccagt atgttggctg acgtgcggct
     7921 gacggagttc aacgaacggg ttgtcctgcg gttcggcgcc gcatacggcg catcggtgct
     7981 ggtagatcac gtgctgaccg gccttgacgg tcggaccgcc gcccgggcga tcgaggacgg
     8041 cgtcgagccc cgcgacgttt ggcgggcgtt gtgcgtcgac ttcgacgtgc cccgcgacca
     8101 gtggtgaggc ggcgttctcg cgccatctcg cgccatcgag tgtgcgctgg tggcgtcgag
     8161 tgtgcgctgg tggcgcgatc tgaacggctt ttccaccctg gatgcacgtc caaagccggg
     8221 aacgcacacc caatgtcggc ggcgcaggct caactccggt tcggccgcgg tgtccggcgg
     8281 aacacgcaat cgccgcaaac ggaccttccg gggacgcgat agaacaggca gcagctgcga
     8341 cgcctgaagc ccagatgcgg ggtggtgatc aggccggatc cggccaacgt gcccgtgtcc
     8401 agtagtgagt tggtgatcgc gacgatgg
//
LOCUS       DASSGQ010000188         2937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_135030,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000188 DASSGQ010000000
VERSION     DASSGQ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135030"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="VFI55_08320"
     CDS             <1..1261
                     /locus_tag="VFI55_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HET7074069.1"
                     /translation="RAGLIELLDIDDIQAQAILDMQLRRLAALERQRIIDDLARIEAE
                     IADLEDILAKPERQRGIVRDELGEIVEKHGDDRRTRIIAAHGDVNDEDLIAREDVVVT
                     ITETGYAKRTKTDLYRSQKRGGKGVQGAGLKQDDIVRHFFVCSTHDWILFFTTQGRVY
                     RAKAYELPEASRTARGQHVANLLAFQPEERIAQVIQIRGYEDAPYLVLATRNGLVKKT
                     KLTDFDSNRSGGIVAINLRDSDELVGAVLCSSDDDLLLVSANGQSIRFSATDEALRPM
                     GRATSGVQGMRFNADDYLLSLNVVREGTYLLVATSGGYAKRTGIDEYPVQGRGGKGVL
                     TVMYDRRRGRLVGALIVDDDSELYAITSVGGVIRTAARQVRKAGRQTKGVRLMNLGDG
                     DTLLAIARNAEENADEVVDEVDGAADS"
     gene            1450..2355
                     /locus_tag="VFI55_08325"
     CDS             1450..2355
                     /locus_tag="VFI55_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HET7074070.1"
                     /translation="MTSPNEPGAPQKGDNLNGDGSAERAGAHRATPAPTGRIQDSGES
                     PPWQRGGAWGAPSAHRPTEPHTEVRPPAPAGGADARLNRFISGTGAPSAGAPPHSKNP
                     PHDFGAGRGDGPPSEAYASELPDLSGPIPRPPQRKTAPESGAEAASTSGSVRPAIPES
                     RDSRETRETRENRVQVSRRTRGPVRASMQIRRIDPWSTLKVSLMLSVALFFVWMIAVA
                     FLYLVLGGMGVWAKLNSNVGDLLNNTSGSSGELVSSGTIFGGAVLIGLVNIVLLTAMS
                     TIGAFVYNLTTDLIGGIEVTLADRD"
     gene            2427..2500
                     /locus_tag="VFI55_08330"
     tRNA            2427..2500
                     /locus_tag="VFI55_08330"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2461..2463,aa:Ile,seq:gat)
     gene            2652..2724
                     /locus_tag="VFI55_08335"
     tRNA            2652..2724
                     /locus_tag="VFI55_08335"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2685..2687,aa:Ala,seq:tgc)
     gene            complement(2745..>2937)
                     /locus_tag="VFI55_08340"
     CDS             complement(2745..>2937)
                     /locus_tag="VFI55_08340"
                     /inference="COORDINATES: protein motif:HMM:NF023900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PPE family protein"
                     /protein_id="HET7074071.1"
                     /translation="PVTAVTNPEVAALNATAVPAGAEGINALPMAPPFGQFSGNRYGR
                     ILPTYGFKPSVMAKPPAAG"
BASE COUNT          523 a          958 c         1000 g          456 t
ORIGIN      
        1 ccgcgccggt ctgatcgagc tgctcgacat cgacgacatt caagcccagg ccatcctgga
       61 catgcagctg cggcggctgg ccgcgctgga gcgccagcgc atcattgacg acctggcgcg
      121 gatcgaagcc gagatcgccg acctggaaga catcctggcc aagccggagc ggcagcgcgg
      181 gatcgtccgc gacgaactcg gcgagatcgt cgagaagcac ggtgatgacc gtcgcacccg
      241 catcatcgcg gcccacggcg acgtcaacga cgaggatctg atcgcccgcg aggacgtcgt
      301 cgtcacgatc accgagaccg gatacgccaa gcgaaccaag accgatctgt accgcagcca
      361 gaagcgcggc ggcaagggcg tgcagggcgc cgggctcaag caggacgaca tcgtccggca
      421 cttcttcgtg tgctcgacgc atgactggat cctgttcttc accacgcagg gccgggtgta
      481 ccgcgccaag gcatacgagc tgccggaggc ttcccggacg gcccggggtc agcacgtggc
      541 caatttgctg gccttccagc ccgaggagcg catagcgcag gtcatccaga tccgcggcta
      601 cgaggacgcg ccgtacctgg tgctggccac ccgcaacggc ctggtcaaga agaccaagct
      661 gaccgatttc gactccaacc gctcgggcgg aatcgtggcg atcaacctgc gtgacagcga
      721 tgagctggtc ggtgcggtgt tgtgctcgtc cgacgacgac ctgctgctgg tctcagctaa
      781 cggtcagtcc atccggttct cggcgaccga cgaggcgctg cgcccgatgg gccgcgctac
      841 ctccggcgtg cagggtatgc ggttcaacgc cgacgactac ctgctgtcgc tgaacgtggt
      901 ccgcgaaggc acctacctgc tggtcgccac ctccggcggc tacgccaagc gcaccgggat
      961 cgacgagtat ccggtgcagg gacgtggcgg caagggcgtg ctgaccgtga tgtacgaccg
     1021 ccggcgtggc aggctggtcg gggcgctgat tgtggacgac gacagcgagt tgtatgcgat
     1081 cacctccgta ggaggcgtaa tccgcacagc tgcgcgccag gtccgcaagg caggacggca
     1141 gaccaagggc gtccggttga tgaacctggg cgacggcgac acactgttgg cgatcgcgcg
     1201 caacgccgag gaaaacgccg acgaggtggt cgacgaagtc gatggcgccg cggattcgta
     1261 gggcgattca ggtaacgatg cgggcaagca gcgacccgcc agagcactcg ggcaattaga
     1321 ctcagcccga cgacgatgcg ggccttggag gggcccgagg aggagttggg taattagact
     1381 cagcccgacc agacataatg cgactgccac cgcgcgggcg gcctggtagg aaatctaagg
     1441 agtgtcgggg tgacctcacc gaacgagccg ggcgcccccc aaaagggcga caacctcaat
     1501 ggggacggct cggccgagcg tgccggtgca caccgcgcga cgcccgcacc gactggccgt
     1561 attcaggatt ccggtgaatc gcctccctgg cagcgtggcg gtgcctgggg cgcgccgtcg
     1621 gcgcatcgcc cgaccgaacc ccacaccgag gtccggccgc cggccccggc cggcggcgcc
     1681 gacgcgcggc tgaaccgctt catctccggc accggcgcgc cgtccgccgg cgcgccccca
     1741 cactcgaaga acccgccgca cgatttcggt gcagggcgcg gcgacgggcc gccctccgag
     1801 gcctacgcca gcgagttgcc agacctgtcc ggccccattc cacgaccgcc gcagcgcaag
     1861 accgcgcccg aaagcggcgc ggaagcggcg agcacgtcgg gatcggttcg tcctgcgatc
     1921 ccggaaagca gagacagccg agaaacccgg gaaactcggg aaaaccgtgt gcaggtgtcg
     1981 cggcgaaccc gcggtccggt tcgggccagc atgcagatcc gccggatcga cccgtggagc
     2041 acgttgaagg tgtccctgat gctctcggtg gcactgttct tcgtttggat gatcgcggtg
     2101 gcgttcctct acctcgtgct cggcggcatg ggcgtctggg ccaagctgaa cagcaacgtc
     2161 ggcgacctgc tgaacaacac cagcggcagc agcggtgaac tcgtctccag cgggaccatc
     2221 ttcggcggcg ctgtgctgat cggcctggtc aacatcgtcc tgttgaccgc gatgtcgacc
     2281 atcggcgcgt tcgtctacaa cctcaccacc gatctgatcg gcggcatcga agtgacgctg
     2341 gccgaccgcg actagcgcgt atccacccgc ttttgggggt cgggccgcga ttgcggtaat
     2401 ctcgtcgctc ggccgtacgc gagtaagggc ctatagctca ggcggttaga gcgcttcgct
     2461 gataacgaag aggtcggagg ttcgagtcct cctaggccca caaccatgtg cccgtcaaga
     2521 cgttgcgtga gacttgcatt gccgctggca atgatcgctg tagcagcagc ggtcgcgtgg
     2581 tcgcgacgcg gcgtcgaggt ctggcacgtg gcggtggccg acgcacccgt gcgcccacct
     2641 ggtgacgacg aggggcctta gctcagttgg tagagcaccg cctttgcaag gcgggtgtca
     2701 ggggttcgat tcccctaggc tccacggatt gacccccgcc tgtgtcatcc tgcggcgggg
     2761 ggcttggcca tcaccgaggg cttgaacccg tacgtcggaa gaattcgccc ataacggttt
     2821 ccgctgaatt gcccgaatgg aggggccatc ggcagggcat tgatgccttc ggccccggca
     2881 gggaccgctg tggcgttgag ggccgccacc tcagggttgg ttaccgcggt caccgga
//
LOCUS       DASSGQ010000189         4523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_500106,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000189 DASSGQ010000000
VERSION     DASSGQ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500106"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VFI55_08345"
     CDS             <1..631
                     /locus_tag="VFI55_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HET7074072.1"
                     /translation="LPWLAAAGVLLVALAVLAMVGDIRRGGPRSDDQPADHGHVHSHR
                     TGIVWLLVVPIVILIFVTPPALRPSAAAPSVTSVSNDVLNRAFPPLPPGRAPEVSLPE
                     VLMREAQDTTGSLTNRTITVTGFVLNEAQGVDLGRIVIICCAADAQLARIHLRGPAAA
                     QTAGLPDNTWLRVEGEVTPRQSNSATIPTLQATMVSRVDAPPNPYAYPH"
     gene            901..1700
                     /locus_tag="VFI55_08350"
                     /pseudo
     CDS             901..1700
                     /locus_tag="VFI55_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305984.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            complement(1745..2602)
                     /locus_tag="VFI55_08355"
     CDS             complement(1745..2602)
                     /locus_tag="VFI55_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET7074073.1"
                     /translation="MGNALIGGTIVALAAGLIGYFVVVRQTAFAAHALAHIGFPGATG
                     AVLLGVPVAVGLGVFCVGGALVIGVLGKRAADREVVTGTVLAMATGLGLFFNSLATKS
                     SSTVTNVLFGNLLAVSHNQLVGFAILLLVLGLGIGIIYRPLLFSSVNALVADAKGVPV
                     RALSMIFMALLGLTITMAVQAVGTLLLFALVVTPAATAIMLTPRPVLAMVASTAISLS
                     AVWLGLGASAMFNLPPSFPIVTIACTVWAVVWAGSRRRGAVQFAERSTEASPATDPSP
                     RPATATRGA"
     gene            complement(2665..3453)
                     /locus_tag="VFI55_08360"
     CDS             complement(2665..3453)
                     /locus_tag="VFI55_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7074074.1"
                     /translation="MSLSELVGAPPALTFTDVSAVRGGLLVWSHATFTVPTGGIVAVI
                     GPNGAGKTTLLQMVLGLIPAATGRLEVFGRPPGQANDRIGYVPQHYADGSGEAIRAAD
                     VVLLGLTGTRWAFGRSTAAQRKQVDRALAAVEATEIAGRRLSTLSGGQRQRVAIAAAL
                     VAQPQLLILDEPLASLDLHSQRDIVALLARLQAELAVTILVVAHDLNPLLPILDSAIY
                     LLDGHPHYAPIDDVVDDVLLTNLYGTPIRIAHHLTGERQMRNAL"
     gene            complement(3440..4231)
                     /locus_tag="VFI55_08365"
     CDS             complement(3440..4231)
                     /locus_tag="VFI55_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HET7074075.1"
                     /translation="MVSVDQWGDIVSELGGSCASVKTVLASSSVDPHDYEPSPADAAS
                     FTDAKLVVVNGAGYDAWAAKLAASSAAAAPVVSAAAVTKTPDGANPHLWYQPAAVPAV
                     ADAVTAALSKLDPQAGDYFSQRRSQFTATMAPYGSLVDKIKAEAAGKSYAATETVFDY
                     QAQALGLVNKTPEGYQRASANESDPAPGDIDAFRTLLAGRGVDVLVYNTQTEGSIPQQ
                     IRAAADSAHVPVVNVTETVPPGQTSFESWQYAQLVALGKALGVAV"
BASE COUNT          719 a         1523 c         1590 g          691 t
ORIGIN      
        1 gctgccgtgg ttggccgccg cgggggtgct gctcgtcgcc ctggcggtat tggcgatggt
       61 gggcgacatc cggcgcggcg gaccccggtc ggatgatcag ccggctgatc acggacacgt
      121 gcactcgcac cggaccggca tagtgtggct gctcgtcgtt ccgatcgtga tcctgatctt
      181 tgtcacaccc ccggcgctga ggccgtccgc tgccgcgcct tcggtgacat cggtgtccaa
      241 tgacgttctg aatcgggcat ttccgccgct gcccccgggc agggctccgg aagtgtcgct
      301 gccggaggtg ctgatgcgcg aggctcagga caccaccggg tcgctgacga atcggacgat
      361 cacagtgacc ggctttgtcc tcaacgaggc gcaaggcgtc gaccttggtc gcatcgtcat
      421 catctgttgc gcggccgacg cgcagctggc gcgcatccat ctgcgtggcc cggctgcggc
      481 acagaccgcc gggttaccgg acaacacttg gcttcgggtc gagggcgaag ttacgcccag
      541 acaatcgaat tcggccacga ttccgacgct gcaggccacc atggtctcgc gcgtcgatgc
      601 tccccccaac ccctacgcct acccgcactg acgcgcccgc ccgggaaggc tcggcgcacc
      661 gcagctcaat tgcgttggcc ggcgccgcgg acccacagct gatgcctgtt gagatgtcct
      721 ggcacgggcg tgttttccca ttggccaagc tccagccaat cgctcagggc cgctgcatca
      781 ccgtcgtcgg tgatgggacc gatccaatgg ggcggcccgc aggctatccg gtcgttcgtg
      841 cagggctgga ccacggccat gacgccacag ccggtcgggc gagaggcgca ggtgagtggg
      901 gctatggacg tctcgatcgc cgcggtggtg acctgtgtcg actcatcggt gtggctgggc
      961 caggcgctcg gtgacgccga gctggacgac ggacgcaccc aggcccaggt ggtggtcggt
     1021 taggccgaat tcgccgatgt ggtcgtgctc aaccggcccc accccgcgca tgcatcactg
     1081 tgggcgccgg cgacgtcgag cgcacgttgg ccaacctgta ccgcgacgcg cggcggggcc
     1141 gcagcgacga tccgcacggt ccgctgctcg ccggacagcc gcccctggat tcgcggggcc
     1201 cgatcaacct gatcgagttc aatgcccggc ggcccttcca tccgcaccgt ctgcacgcgt
     1261 gtctggatct gctgctcgac ggcgtcatcc gaacccgggg acggctgtgg ttggccagcc
     1321 ggcccgagca ggcgatgtgg ttggaatccg ccggcggcgg tttgcgggtc agctcggccg
     1381 gcaaatggct ggccgccctg acgggccctg agctggccgg cgtcgatgcg gaacgacgcg
     1441 ccttcgccga gctcgggtgg gacgaacggc acggcgatcg gcacacggcg atgaccgtcc
     1501 tggtgtgcgg cgcgcacgcc gccgaaatcc tcgacggtct ccacggcgcg ctgctcaccg
     1561 acgaggagtt cggctcccca caggactggc tccactacga cgatccattc ggcgactggc
     1621 acgaagaccc ctgcgcggac ctggcggatg aaacccagga cgcgcctgct caccacaccc
     1681 aggacggaga ccgcggatga gagaaactcc gccgccgcta tggcagtcgg cggggcgata
     1741 gccgtcaagc cccccgggta gccgtggccg gcctgggcga cgggtccgtc gctggcgagg
     1801 cttcggtgga tctttccgcg aattgaacgg cgccccgacg gcggctgccg gcccagacga
     1861 ccgcccagac ggtgcaggcg atggtgacga tggggaagct cggcggcagg ttgaacatcg
     1921 ccgaagcgcc cagtccgagc cacaccgcgc tcaggctgat cgccgtcgag gcgaccatgg
     1981 ccagcaccgg ccggggggtc agcatgatcg ccgtcgcggc gggggtgacg accagcgcga
     2041 acagcagcag cgtgccgacg gcctgaacgg ccatcgtgat ggtcagcccg agcagcgcca
     2101 tgaagatcat cgacaatgcg cgcacgggca cgcctttggc gtcggcgacc agggcgttga
     2161 ccgagctgaa cagcaggggg cggtagatga tgccgatgcc gagccccagc accagcagca
     2221 ggatcgcgaa gccgaccagt tggttgtggg agaccgccag caggttgccg aacagcacgt
     2281 tggtcaccgt gctggagctc ttggtggcca gcgagttgaa gaacagaccg aggccggtcg
     2341 ccatggccag gacggtcccg gtgaccactt cgcgatcggc ggcgcgcttg cccagcacgc
     2401 cgatcaccag ggccccgccg acgcagaaca cgcccagccc caccgcgacc ggcacgccca
     2461 gcaggaccgc cccggtcgcg cccggaaatc cgatgtgagc cagcgcgtgg gcggcgaagg
     2521 cggtctgacg caccaccacg aagtagccga taagccccgc cgccagcgcg acgatggtcc
     2581 cgccgatcag ggcgttgccc atgaatcccg aggtcagaat cggccaccaa ttgggttggt
     2641 aggccatggc gacgacgtgg gcggtcacag tgcgttccgc atctgccgct cgccggtgag
     2701 gtgatgcgcg atccgaatcg gagtgccgta cagattggtc agcaacacat cgtccacgac
     2761 gtcgtcgatg ggcgcgtagt gcggatgccc gtccagcagg tagatcgcgc tgtccaggat
     2821 gggcagcagc gggttgagat cgtgggcgac caccaggatg gtcacggcca gctcggcttg
     2881 cagacgggcg agcaatgcga cgatgtcgcg ctggctgtgc agatccagcg acgccagcgg
     2941 ttcgtcgagg atcagcagct gcggttgcgc caccagagcc gcggcgatcg cgacgcgttg
     3001 acgttggcct ccggacagtg tcgacaaccg ccggccggcg atctcggtgg cctccacggc
     3061 ggccaacgcc cggtcgacct gcttgcgttg ggccgcggtg ctgcgcccga acgcccaccg
     3121 ggtcccggtg agcccgagca gcaccacgtc ggcggcgcgg atggcttccc cagacccgtc
     3181 ggcgtagtgc tgcggtacgt agccgatcct gtcgttggcc tggccgggtg gacggccgaa
     3241 cacctccagc cggccggtgg ccgccgggat caggccgagt accatctgca gcagggtggt
     3301 cttgccggcc ccgttgggtc cgatgacagc gacgatgccg ccggtcggca cggtgaaggt
     3361 ggcatgcgac cagacgagca gtccgccgcg taccgcgctg acgtcggtga acgtcagcgc
     3421 gggcggagcg ccaaccagct cagacagcga caccgagcgc cttgcccaat gcgacgagct
     3481 gggcgtactg ccagctttca aacgaggtct gcccgggcgg cacggtctcg gtcacgttga
     3541 cgaccgggac gtgcgcactg tcggcggcgg cacggatctg ctgggggatc gaaccctcgg
     3601 tctgggtgtt gtagaccaac acgtcgacgc ctcggccggc caggagcgtg cggaacgcgt
     3661 cgatgtctcc cggtgcggga tccgattcgt tggccgaggc ccgctgatag ccctcggggg
     3721 tcttgttgac caggcccaac gcctgtgcct gatagtcgaa caccgtttcg gtggccgcat
     3781 acgacttgcc cgcggcttcg gccttgatct tgtcgacgag gctgccgtag ggcgccattg
     3841 tggcggtgaa ctgcgatcgg cgctggctga agtagtcgcc ggcctgcggg tcgagcttgc
     3901 tcaacgcggc ggtcaccgcg tcggctaccg ccggcaccgc cgccggctga taccacaggt
     3961 gcgggttcgc gccgtcgggt gtcttggtga ccgcggcggc actcaccacg ggggccgcgg
     4021 cggcggagct ggcggcgagt ttggctgccc aagcgtcgta gccggctccg ttgaccacga
     4081 caagcttggc gtccgtgaag gacgccgcgt ccgccggcga aggctcgtaa tcgtgcgggt
     4141 ccaccgagga gctggccagc accgttttga cgctcgcgca cgagccgccc agttcggaca
     4201 cgatgtcgcc ccactggtcg acgctgacca ccacggctac cggcgcgcca gggcaggacg
     4261 ccgcgtcggt gttgcccgcc ttggtctggc tcgtcgaaca ccccgtcacc gcaagcgcga
     4321 gcgcggcggc gccgacagac gcgcggcgca accgggtgga tacgacagtc acaacgagaa
     4381 cgataaccgt tttcatcatg gctcggcaga ggccaaccca acatcgtgag caaacggccg
     4441 agctatccgg gtgatgcgag cgcccgatag cccggctcgg cgaaacgctg tgcggcgtcg
     4501 cgcatctcct gagttatctg gtg
//
LOCUS       DASSGQ010000190         8510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1046422,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000190 DASSGQ010000000
VERSION     DASSGQ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8510
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046422"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..343
                     /locus_tag="VFI55_08370"
     CDS             59..343
                     /locus_tag="VFI55_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074076.1"
                     /translation="MDVHLSSHHRDTLGKIFTHPTSGNIEWREVLSLLDYVGTTAEER
                     NGKFRVTLGPETEVLEAPRGKDIDAQMVVDLRRLLRQAGLAPDDGDADTT"
     gene            complement(450..752)
                     /locus_tag="VFI55_08375"
     CDS             complement(450..752)
                     /locus_tag="VFI55_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074077.1"
                     /translation="MNPVGMRWSVLGAVPVALAGTAVAVFIGLSGMESQPAIPHTVSS
                     VPGSIRLADSDQDQLNQMEQQQAQQTAQQSEDLAQQEQQQVDQQNAFNESMTAGNN"
     gene            complement(900..1724)
                     /locus_tag="VFI55_08380"
     CDS             complement(900..1724)
                     /locus_tag="VFI55_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HET7074078.1"
                     /translation="MKKGLKREAALAAAMLAAGSAALGACTNERSSQSTTSPATGAPP
                     GTESMTTQVKSADGTLVANATIDFANGYATVTLEAVPNQVLSPGFHGLHIHSVGKCEA
                     NSVAPTGGAAGDFNSAGGHFQAPGHTSYPASGDLTSLEVRSDGSAKLVTTTNSFTAAD
                     LRNSSGTALIIHQDPDNFGNIPAERYTQKNGTPGPDAETLATGDAGRRVACGVIAATA
                     ATTTSSTATTPTVTTTTTTVEVPAPSTSTSTVTVTSVPAVTPTPTKTIPTTSLPPG"
     gene            complement(1811..2272)
                     /locus_tag="VFI55_08385"
     CDS             complement(1811..2272)
                     /locus_tag="VFI55_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET7074079.1"
                     /translation="MTTREIGREAATSEGTVTVAETGTGTYTQQITVGQHRLVADEPQ
                     PIGDDAGPTPYDLLLAALGACTSMTIRMYANRKGWPLERVRVTLRHSRIHAKDCANCE
                     TTDGWIDHIDRSIGLTGDLDETQRQRLLLIAERCPVHQTLTSEVDVATSLR"
     gene            complement(2318..3166)
                     /locus_tag="VFI55_08390"
     CDS             complement(2318..3166)
                     /locus_tag="VFI55_08390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET7074080.1"
                     /translation="MSASDTATNDLGRFGFFGLGATPEQAQEIERLGYGTVWVGGSPP
                     AELSFVEPLLASTTTLKVATGIVNIWTAAAKPVAESFHRINAAYPGRFVLGIGVGHPE
                     AQAQYRKPYEALESYLDELDEYGVPADQRVLAALGPRVLRLSAERSAGAHPANATPEH
                     AARARAIIGPNALLAPVHNVVLTSDADEARAIGRTALDRYFGLTNYVSSWKRLGFTDA
                     DVARPGSDRLIDALVAYGTPDDIARRLDEYLSAGANHVALLVHGAPEKVLPTLSELAG
                     PLGLSR"
     gene            3336..4076
                     /locus_tag="VFI55_08395"
     CDS             3336..4076
                     /locus_tag="VFI55_08395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7074081.1"
                     /translation="MAGIEGKVVAITGASSGIGEATGRLLAQRGAAVVLGARRVERLD
                     ELAGEIRQRGGQAVACAADVARRADLERLVARAAETFGRLDVLVSNAGISKIGPLSGL
                     DVDGWSTMIDVNLRGVLYGIAAAYPVFQRQGQGHFVTTVSTAGLKIVPGMAVYAATKN
                     AVRTVMEALRQESTDGVVRTTAISPGYVRTELDASIDDPEVREQIRHNMNDFGIEPEA
                     VARAVAFAIEQPHDVEIGEITIRPTVQG"
     gene            complement(4202..4510)
                     /locus_tag="VFI55_08400"
     CDS             complement(4202..4510)
                     /locus_tag="VFI55_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HET7074082.1"
                     /translation="MPQPEQLPGPNADIWNWQLQGLCRGVDSSMFFHPDGERGRARMQ
                     REQRAKEMCRRCPVIEQCRSHALEVGEPYGVWGGLSESERDLLLKGDIGRNRGIRRSA
                     "
     gene            4866..5687
                     /locus_tag="VFI55_08405"
     CDS             4866..5687
                     /locus_tag="VFI55_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074083.1"
                     /translation="MIESPHTSKIDRKLAAAAFGDRPDSWPLPAATTPLQLWLRAVAA
                     GGQGRYGSAYGDLAELRRAGPAGRLVSLAHSTQASFLRQLGWHTLARGWDGRAMALAG
                     TDPEARADALIGLAADALGVGRLAAAATLLTRADAALVSGPEVSDRLPVRRQWVAAEL
                     AMVSGEGATAVGHAEAAVELTRAMAVPSARHRVKSEVVLAAALCSAGATGRARAVAEA
                     ALDATGRLRLLPLRWALACLLIDIGNDTLSAQKLREIRDICAGQVRRAGGAWRSA"
     gene            5769..6344
                     /locus_tag="VFI55_08410"
     CDS             5769..6344
                     /locus_tag="VFI55_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003917787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7074084.1"
                     /translation="MTIQAERLDAVVAEAVAGDPKALREVLETIRPIVVRYCRARVGP
                     AERGGLSADDVAQEVCLATITALPRYRDRGRPFLAFLYGIAAHKVADAHRAAGRDLAY
                     PAESVPERWSSDAGPEQMAIEADSVSRMSELLEILPAKQREILILRVVVGLSAEETAA
                     AVGSTTGAVRVAQHRALSRLKSEIVAAGDYA"
     gene            6337..7146
                     /locus_tag="VFI55_08415"
     CDS             6337..7146
                     /locus_tag="VFI55_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-D factor RsdA"
                     /protein_id="HET7074085.1"
                     /translation="MPEYDFARGDHSALDEMTRTDLLLDALAERRSIDLDDPSDPNFE
                     ALAALLEDWRDNLRWPPASALVSPEEAFGALREGMAERRHARRGLAAVGSVAATLLVL
                     SGFGAMVVEARPGDTLYGLHAMFFDQPRVNDDQITLSAKADLAKVQQMIDQGQWSQAQ
                     NQLAEVSSTVQSMDDGTSRHDLMDEVNLLNTKVESRNPNATLPASATQVPSAPVASTP
                     PRVSPSPSVKPPTAATPPSPSASASPSPSSGRHRHHWQTPTPSSPAPDGAQ"
     gene            complement(7159..7569)
                     /locus_tag="VFI55_08420"
     CDS             complement(7159..7569)
                     /locus_tag="VFI55_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 domain-containing protein"
                     /protein_id="HET7074086.1"
                     /translation="MRDHLPPGLPPDPFADDPCDPSAALDAVEPGQPLDHQERMAVEA
                     DLADLAVYEALLAHKGIRGLVVCCDECQQDHYHDWDMLRANLLQLLIDGTVRPHEPAY
                     DPEPDSYVTWDYCRGYADASLNEATSDADGFRRH"
     gene            7797..>8510
                     /locus_tag="VFI55_08425"
     CDS             7797..>8510
                     /locus_tag="VFI55_08425"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET7074087.1"
                     /translation="MSRGTSHLEDSSPLVASSFVRGAPVSGLADAPVPTGGDDPHKVA
                     MLGLTFDDVLLLPAASDVVPAAADTSSQLTKKIRLKVPLVSSAMDTVTESRMAIAMAR
                     AGGMGVLHRNLPVAEQAGQVEMVKRSEAGMVTDPVTCRPDNTLAQVEAMCARFRISGL
                     PVVDESGALVGIITNRDLRFEVDSTRHVAEVMTKAPLITAQEGVSADAALGLLRRNKI
                     EKLPVVDGHGRLTGLITVKD"
BASE COUNT         1389 a         2738 c         2907 g         1476 t
ORIGIN      
        1 aaggggcgtg ggaatgtgac acgatcgagt tcgtctatcc agctcggcag gacggcccat
       61 ggacgtgcat ctcagcagtc atcaccggga tactctcggg aaaatcttca cccatccgac
      121 cagcggcaat atcgagtggc gtgaagtttt gtcgctgctc gattacgtcg gaacgactgc
      181 cgaagagcgc aatggcaaat ttcgagtgac gttgggtccc gaaactgaag tgctcgaggc
      241 gccgcgtggg aaagacatcg acgcgcagat ggtcgtggac ctgcgccggc tgctcaggca
      301 ggcagggctg gcaccggatg atggagacgc agacaccaca tgaaggctgt cccgatggtt
      361 tcgtcctact ggtgcgggta cgcgccattg ccgagcccgc gaccgaatga cgctgagcgg
      421 cgacttcagt cgcggtcggt tcacgtgcgt tagttgttcc cagccgtcat cgactcattg
      481 aatgcgttct gctgatcgac ctgctgctgc tcttgctggg cgagatcttc ggattgctgc
      541 gccgtctgct gagcctgctg ttgctccatc tgattcagct gatcctggtc ggaatcggcc
      601 aacctgatgg aacccgggac cgacgaaact gtgtgcggga tcgcgggttg agattccatc
      661 ccgctcaatc cgataaacac cgccaccgcc gtcccggcca gggcaaccgg cacggcacct
      721 aagacactcc atcgcatccc gactgggttc atcttgactt ccttctgggt gggctcgtcg
      781 aaccaccgat tggttcgagt gaggccaaac ctagggcggc gacctgggca gggggcctgc
      841 tggttttgtg ggtgagctat gaatcacgct cgtccgggag gcttcgtccg ttcagcacgt
      901 cagcccggcg gaaggctggt agtcggtatc gtcttcgtcg gcgtgggcgt gacggccggg
      961 acgctggtaa ccgtgacggt gctggtgctc gtggacggcg ccggcacctc gaccgtcgtc
     1021 gtggtcgtcg tgacggtagg tgtcgtggcg gtggacgagg tcgtcgtcgc cgccgtcgcg
     1081 gcaatcacac cgcaggccac ccgcctgccc gcgtcaccag tggccagcgt ctctgcgtcc
     1141 gggcccgggg tgccgttttt ctgggtgtag cgctcggcag ggatattgcc gaagttatcc
     1201 gggtcctggt gaatgatcag cgcagtaccc gagctattcc tcaggtccgc cgcggtgaac
     1261 gagttggtcg tggtgacgag cttcgccgag ccgtcggagc gcacttccag agaggtcagg
     1321 tcgccgctgg cggggtaact ggtgtggccc ggcgcctgaa aatggccgcc ggcggagttg
     1381 aagtccccgg ccgcgcctcc cgtgggcgca accgaattgg cctcgcattt gcccaccgag
     1441 tggatatgca gcccgtggaa acccggggac aagacctggt tgggtacggc ctcgagggtc
     1501 accgtcgcgt agccattcgc gaaatcgatg gtggcgttgg cgaccagcgt cccgtcggcg
     1561 ctcttcacct gcgtggtcat gctctctgtg ccgggcggcg cgccggtggc cggtgacgta
     1621 gtggattggc tactgcgctc gtttgtgcat gcgcctaacg ctgcgctgcc cgcggcgagc
     1681 atcgcggccg ctaatgctgc ttcccttttc aagccctttt tcatggcctg ggcctacccc
     1741 gatgggccgc cgtcaaaacg gcgcggccca tcgatgacga caagaccacg cacggctata
     1801 tgcgcagctg ctagcgcaag gacgtggcga cgtccacctc tgaggtcagg gtctgatgaa
     1861 ccggacagcg ttcggcgatg agcaacaacc gctgccgctg agtctcgtcg aggtcgccgg
     1921 ttaacccgat ggatcgatca atatgatcga tccatccgtc ggtagtttca cagttggcgc
     1981 agtctttcgc gtgaatgcgt gagtgccgca acgtcactcg aacccgctcg agtggccagc
     2041 ccttacggtt ggcgtacatc cgaatggtca tcgaggtaca tgccccaagc gcggccaaca
     2101 gcagatcgta gggtgtcggc cccgcgtcgt cgccgatcgg ctgtggctcg tcggcgacga
     2161 gccgatgctg tccgacggtg atctgctggg tataagtccc cgtacccgtc tccgcgacgg
     2221 tcaccgtgcc ctcggacgtc gccgcttccc ggccgatttc gcgtgttgtc acgccactct
     2281 cctcgagcgt tggaatgccg cgccaagctg caattctcta acgggacagg ccaagcggcc
     2341 cagcgagttc ggacaacgtc ggcaacacct tctccggcgc gccgtgtacc aggagcgcga
     2401 cgtggttggc gccggcgctc agatactcgt ccaggcgcct ggcgatgtcg tcgggcgtcc
     2461 cgtatgcgac caaggcgtcg atcagcctgt cgctgccggg cctggcgacg tcggcatcgg
     2521 tgaaacccaa ccgcttccag ctgctcacat aattcgtcag gccgaagtag cggtccagcg
     2581 ccgtgcgccc gatggcgcgt gcctcgtcgg cgtcactggt cagcaccaca ttgtgtacgg
     2641 gcgccagcag tgcgttcggc ccgatgatcg cgcgggcccg cgcggcgtgt tcgggcgtgg
     2701 cgttggcggg atgcgccccg gcgctgcgtt cggccgacag tctcagcacc cgcggcccga
     2761 gcgccgcgag cacacgttga tcggccggta cgccgtattc gtcgagctcg tcgaggtatg
     2821 actccagggc ctcgtacggc ttccgatatt gcgcctgggc ctcgggatgg ccgaccccga
     2881 tgccaagcac gaagcggccg gggtaggcgg cgttgatgcg gtgaaacgac tcggcgacgg
     2941 gcttggcggc cgccgtccag atgttgacga tgccggtggc caccttcagc gtggtagtcg
     3001 acgccaaaag cggctcaacg aaggacaatt cggccggcgg cgaaccgccg acccaaaccg
     3061 tgccgtagcc tagccgttcg atctcctgcg cttgctcggg cgtggcgccg agtccgaaaa
     3121 aaccgaatcg gccgaggtcg tttgtcgcgg tgtctgatgc gctcattgcc agtgcaaccc
     3181 ccgcctcgcg cgaggctatt ccttcttggt ccgtcttctc gcgagacgcc cccgcccggt
     3241 tgctcaccga gggcgcgcgt ggccgcaatc gccggtcagc gagactcgat caggtaaccg
     3301 gcgcgagcgg tgcgccgaga tggtaagggg tcgaaatggc gggtatcgag ggcaaagtcg
     3361 tcgcgatcac gggagcaagc agcggcatcg gcgaggcgac ggggcgcctg cttgcgcagc
     3421 gtggtgcagc ggtggtgctc ggtgcacgcc gggtcgagcg acttgatgaa cttgccggtg
     3481 agattcgcca gcgcggtggc caagctgttg cctgtgccgc ggacgttgcc cgccgcgccg
     3541 acctcgagcg actcgtcgcc cgcgcggcgg agacgttcgg gcggctcgat gtgctggtga
     3601 gcaacgccgg tatcagcaag atcgggcccc tgtcgggtct tgacgtcgat ggatggtcaa
     3661 cgatgatcga tgtcaatctc cgtggggtgc tctatggcat cgccgcggca tatccggtgt
     3721 tccagcgaca aggccagggt cacttcgtga cgaccgtgtc gactgccgga ctaaagatcg
     3781 tgcccggcat ggcggtgtat gcggcaacca agaatgccgt gcgcaccgtc atggaagcgc
     3841 tgcgccagga atcgaccgac ggcgtggtgc gaaccacggc gatatcgccg ggctatgtcc
     3901 gcaccgaact cgacgcgtcg atcgatgacc ccgaggtccg ggagcaaatc cgacacaaca
     3961 tgaatgactt cggcatcgag ccggaggccg tggcacgagc ggtcgccttc gcgatcgaac
     4021 agccacacga cgtcgagatc ggcgagatca ccattcgccc cacggtgcag ggttgatcaa
     4081 cgcgggtgca cgagccggcg agcacctgtc aacgaacgtt gaggcgtgcg cgccggcgca
     4141 atggcagggc cggccgaccc acccgttgag gcgggccgac cgggtcaggc aatcgtgccg
     4201 atcaggccga gcggcggatg ccgcggttgc ggccgatgtc gcccttcagg agcaggtcgc
     4261 gctccgattc cgacaggccg ccccaaaccc cgtacggctc gccaacttcg agcgcgtgag
     4321 aacggcattg ctcgatcacc gggcaacgcc ggcacatctc cttggcgcgc tgctcgcgct
     4381 gcatgcgggc gcggccacgc tcgccgtcgg ggtggaaaaa catcgaagag tcaacgccgc
     4441 ggcacaggcc ttgcaattgc cagttccata tgtcggcgtt gggtccaggt agttgctccg
     4501 gttgtggcat tggatcattc cctctctgca cggcaccgcc cagaaggcca ggttcgcgaa
     4561 gcgacagtgc aactgatttg gagtggagtc accgatgact acccgtcaga cctagaactt
     4621 aggggcgacg gcgaatttcc gtcaatagcc cgttagcccg ccaaaatata taagcgttgg
     4681 agcagaattt actcgccgtt catctgctaa cgatttgtcg accgagagcc gtgctaccag
     4741 gtacttgatc aaactgagat ttcccagctc aggccgcagg ggggcgcgga acgcaattag
     4801 tacacgccgg atggcgatta acagttcggt aacacagaca ccacgaagtg tttactacta
     4861 tcaccgtgat tgaatctcct cacacttcga agattgaccg aaagttggcc gccgccgcct
     4921 tcggggaccg gcccgacagc tggccgttgc cggccgctac cacgccactt caactgtggt
     4981 tgcgcgcggt cgccgcgggc gggcaaggcc gctacggcag cgcttacggc gatctggcgg
     5041 agctgcgtcg cgccggtccg gccggtcggt tggtttcgct ggcgcacagc acccaggcgt
     5101 cgtttttgcg ccagctcggc tggcacaccc tggcgcgagg ctgggacggg cgcgccatgg
     5161 cgctggcggg caccgatccc gaggcccgcg ccgacgcgct gatcgggctg gccgccgacg
     5221 cgctgggcgt cggccgcttg gcagccgccg caacactatt gactcgcgcg gacgccgcgc
     5281 tggtgtccgg gccggaagtt tcagaccggc ttccggtgcg ccggcaatgg gtggccgccg
     5341 agctggcgat ggtgtccgga gaaggcgcga cggccgtggg tcacgccgag gcggcggtcg
     5401 agctgacccg ggccatggcc gtcccgtcgg cgcgacaccg ggtcaaaagc gaggtggtgc
     5461 tggccgccgc cctgtgtagc gccggcgcca ccgggcgcgc ccgcgcggtc gcggaggcgg
     5521 cgctggacgc caccggcagg ctgcgactgc tgccgctgcg ctgggcactg gcttgcttgc
     5581 tgattgatat cggaaacgac acgctttcgg cacagaagtt gcgtgaaatc cgggatatct
     5641 gcgcaggcca ggtgcggcgc gccgggggcg cgtggcgttc cgcttgaaac ggcctccggg
     5701 ccgttattgt cattgcggta gttagcccgc gatgtgtccc gttcgtaacg ttggagatac
     5761 cgccgtcgat gacaattcaa gcggaacgtc tcgacgctgt ggttgcggag gccgtggcgg
     5821 gggacccgaa ggcactacgg gaggtgctgg agaccatccg tccgatcgtc gtgcgatatt
     5881 gccgcgccag agtcggccca gccgagcggg gtggcctgtc agcggacgac gtagctcagg
     5941 aggtgtgctt ggccaccatc accgcgctgc cgcgataccg ggaccggggg cgcccctttt
     6001 tggccttcct gtacggcatc gcggcccata aggtcgccga cgcgcaccgg gccgccggcc
     6061 gtgatctcgc ctaccccgcc gaatcggttc ccgagcgctg gtcgtcggac gcgggaccgg
     6121 agcagatggc catcgaagcc gactcggtca gccggatgag tgaactgctc gagatcctgc
     6181 ccgccaagca gcgcgagatc ctgatcctgc gcgtggttgt cgggctgtcc gccgaggaga
     6241 ccgccgcggc cgtcggcagc accacgggcg cggtccgggt ggcccagcac cgtgcgctgt
     6301 cacggctcaa gtccgagatc gtcgcggcgg gtgactatgc ctgaatatga tttcgcgcgc
     6361 ggcgaccact ccgcgctgga cgaaatgacc cgcaccgatc tgctcctcga cgcgctcgcc
     6421 gaacgccggt cgattgacct cgacgacccc agcgacccca attttgaggc gctggccgcc
     6481 ctgctggagg actggcgcga caacctgagg tggccgccgg ccagtgcatt ggtatcgccc
     6541 gaggaggcct tcggagcgct gcgcgagggg atggccgagc gccggcatgc ccgacgcggc
     6601 ctggcggccg tcggatcggt tgccgcgacc ctgttggtcc taagcgggtt cggggccatg
     6661 gtggtcgagg cccgccccgg ggacacgctg tacggcctgc acgcgatgtt cttcgaccag
     6721 ccgagggtca acgacgatca gatcacgctg tccgccaagg ccgatctggc gaaggttcag
     6781 caaatgatcg accagggcca atggagtcag gcgcagaatc agctggccga ggtcagcagc
     6841 accgtgcaat ccatggacga cgggaccagc aggcacgacc tgatggacga ggtgaatctg
     6901 ctcaacacca aggtggaatc acgcaacccg aacgctacgt tgccggcctc agcgacgcag
     6961 gtgccgagcg ccccggtcgc ctcgaccccc ccgcgggtct cgcccagtcc gagcgtcaaa
     7021 ccgccgaccg cggccactcc gccgtctccg tcagcgagcg cgtcaccgag cccgtcgtcg
     7081 ggtcggcatc ggcatcactg gcagacgccc acgccgtctt cgccggcgcc ggacggcgca
     7141 cagtaagctg cggggagctc agtggcggcg aaatccgtcg gcgtctgatg tcgcttcgtt
     7201 gagcgaggcg tcggcgtatc cccggcagta atcccatgtg acgtaggagt ccggttcggg
     7261 gtcgtaggcg ggctcgtgtg ggcggacggt gccgtcgatc agcagttgca gcaggttggc
     7321 ccgcaacatg tcccaatcgt ggtagtggtc ttgctggcac tcgtcgcagc acacgacgag
     7381 tccgcgaatc cccttgtgcg ccaacagggc ctcgtacacg gccagatcgg cgaggtcggc
     7441 ctcgaccgcc atccgctctt ggtgatccag gggttgtccg ggctcgacgg cgtccagtgc
     7501 cgccgacggg tcgcaggggt cgtcggcgaa tgggtcgggc ggcaaacccg gcgggaggtg
     7561 gtcgcgcacg ccctctagcg tacgcaagcg ggccgtcaaa acgccagcag gacgcgaccc
     7621 gccgtttcgt agcgtggggc gggtgggacg cgcgggaaag cgagtcccgg ctgcacccta
     7681 ttgccggcta ttgcgcggcg ctgtcccgcg ccgtcccacg gcgtgcatcg ccactggaag
     7741 aagtcgagcc gataggatgg gtttctcact ccggacgcgt atggagggcc ccgccgatgt
     7801 cccgtggcac gtcccacctt gaagacagct ccccgctggt cgccagctcg ttcgtgcgcg
     7861 gcgctccggt ttccggcctt gccgatgcac cggtgcccac cgggggcgac gacccgcaca
     7921 aggtggcgat gctggggctc accttcgacg acgtcctgct gctgcccgcc gcttctgatg
     7981 tggttccggc cgccgcggac acctccagcc agctgaccaa gaagatcagg ctcaaagtgc
     8041 cgctggtcag ctcggcgatg gacacggtca cagagtcacg gatggccatc gcgatggccc
     8101 gggcgggtgg catgggtgtg ctgcaccgca acctgccggt cgcggaacaa gccggccagg
     8161 tcgaaatggt gaagcgctcc gaagccggga tggtcaccga tccggtcacc tgccgaccgg
     8221 acaacaccct ggcccaggtc gaggcgatgt gcgcccggtt ccggatctcc gggctgccgg
     8281 tggtcgacga gtccggcgcg ctggtcggca tcatcaccaa ccgcgatttg cgattcgagg
     8341 tcgactcgac gcgccacgtc gccgaggtga tgaccaaggc cccgctgatc accgcgcagg
     8401 agggtgtcag cgccgacgcg gcgctggggc tgttgcgccg caacaagatc gagaagctgc
     8461 ccgtcgtcga cggtcacggc cggctgaccg ggctgatcac cgtcaaagac
//
LOCUS       DASSGQ010000191         8276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_864515,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000191 DASSGQ010000000
VERSION     DASSGQ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8276
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864515"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VFI55_08430"
     CDS             complement(<1..1193)
                     /locus_tag="VFI55_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET7074088.1"
                     /translation="MFDTVLVANRGEIAVRVIRTLRRLGIRSVAVYSDADVTARHVIE
                     ADEAVRLGPAAARESYLDIGRVLDAAVRTGAQAIHPGYGFLSENADFAAACERIGVVF
                     LGPPAQAIEVMGDKITAKNAVCAFDVPVVPGVAKAGLTDDDLVAAAAEVGYPVLIKPS
                     AGGGGKGMRMVDEPARLREALVSARREAASSFGDDTLFLERFVLRPRHIEVQVLADTH
                     GNVVHLGERECSLQRRHQKVIEEAPSALLDPQTRARIGAAACNTARSVDYVGAGTVEF
                     IVSAALPDEFFFMEMNTRLQVEHPVTEAVTGLDLVEWQLRVAAGEKLAFAQDDIELRG
                     HAIEARVYAEDPARGFLPTGGQVLQVFEPSGDGVRVDSSLLPGTLVGSDYDPMLSKVI
                     AYGTDR"
     gene            complement(1199..2794)
                     /locus_tag="VFI55_08435"
     CDS             complement(1199..2794)
                     /locus_tag="VFI55_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET7074089.1"
                     /translation="MDTVVANLDQATSFADQHRRLVGELNAKLAAAALGGNERARERH
                     VSRGKLLPRERVDRLLDPGSPFLELAPLAAGGMYGDESPGAGIITGIGRVSERECVIV
                     ANDATVKGGTYYPMTVKKHLRAQEVALQNLLPCIYLVDSGGAFLPRQDEVFPDREHFG
                     RIFYNQATMSAKGIPQVAAVLGSCTAGGAYVPAMSDEAVIVREQGTIFLGGPPLVKAA
                     TGEIVTAEELGGGDLHSRVSGVTDHLADDDEDALRIVRSIAATFGPREPSQWEVRRSV
                     EPTHPQTELYDVVPPDPRTPYDVHEVIVRLVDGGEFTEFKAKYGKTLVTAFARIHGHP
                     VGIVANNGVLFSESALKGAHFIELCDKRKIPLLFLQNIAGFMVGRDYEAGGIAKHGAK
                     MVTAVACARVPKLTVVIGGSYGAGNYSMCGRAYSPRFLWMWPNARISVMGGEQAASVL
                     ATVRGEQLAASGSPWSADEEEAFKAPIREQYENQGNPYYSTARLWDDGIIDPADTRTF
                     VGLALSVCAHAPLDSVSYGVFRM"
     gene            complement(2906..4546)
                     /locus_tag="VFI55_08440"
     CDS             complement(2906..4546)
                     /locus_tag="VFI55_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7074090.1"
                     /translation="MAATGAADNPLSFDRGPTLPALLETTIGANFADTAATYAQRDAL
                     VDVAAERRWTYAELRAAVRRLATGLVCAGIGVGDRVGIWSPNRWEWVLVQYATAEIGA
                     ILVTVNPAYRASELEYALRQSGVSMVIAAPRFKTTDYAAMLGEVAPRCLGLRDVVLLD
                     SDRWDELAGTQVDPPLLDEIAATLDRHDPINIQYTSGTTGYPKAATLSHRNILNNGYL
                     VGELLEYTAQDRICIPVPFYHCFGMVMGNLAATSHGACMVIPSPAFEPAATLRAVQAE
                     RCTSLYGVPTMFIAELGLPEFADYELSSLRTGIMAGSPCPVEVMRKVIERMHMPGVSI
                     CYGMTETSPVSTQTRTDDSLERRVGTVGRAGPHLEIKVADSTTGQTVPRGVAGEFCTR
                     GYSVMAGYWNDPEQTAQVVDADGWMHTGDLAVMDACGYIEITGRIKDIVVRGGENISP
                     REIEEFLHTHPDIVDGHVIGVPDARYGEELMAVVMLRDGAPKLTIEGLRDFCAGRIAR
                     FKVPRYLWIVDDFPMTVTGKVRKTEMRQRAIEYLRGRD"
     gene            4688..5344
                     /locus_tag="VFI55_08445"
     CDS             4688..5344
                     /locus_tag="VFI55_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7074091.1"
                     /translation="MATSGPASQPGRPDAPNRRSQLKSDRRLQLLSAAERLFAERGFL
                     AVRLEDIGAAAGVSGPAIYRHFPNKESLLVELLVGISTRLLTGARDVKARNGDAAAAL
                     NDLVDFHLAFALGEPDLIRIQDRDLAHLPETAAKQVRKAQRQYVEIWVGVLRELNPKL
                     AEADARLTAHAAFGLLNSTPHSMKSPDNSRGRPPTAARSRAVMRAMTVAALRAGDHSP
                     "
     gene            5413..6213
                     /locus_tag="VFI55_08450"
     CDS             5413..6213
                     /locus_tag="VFI55_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmpS family transport accessory protein"
                     /protein_id="HET7074092.1"
                     /translation="MNDPRRSERFIPPYPGSGPEGPQGPQYPPPVDPAYADQAPYAPT
                     YGGPIPQWAPSFNETDQTRHLPPYWQHDMPHSGGLPPEGLAPPPPQGPKSPRWLWIAA
                     GAAVLLVVALVIALILANDAIKTQTAVPPLPAMPEPSPTTPSPSTRTPPLPIPPLFPL
                     PTPPTNGSGTPTQTTGPAAMQSVVYNVTGEGRAISIMYIDTGDVIETEFNVALPWSKE
                     VSLSKSALHPANVTIVNIGHNVTCSVTVGGVQVSQRVGVGLTICDARG"
     gene            complement(6210..7559)
                     /locus_tag="VFI55_08455"
     CDS             complement(6210..7559)
                     /locus_tag="VFI55_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7074093.1"
                     /translation="MSQPGSGSATVARSRVAAWALWDCGSTGLNAIVATFVFSVYLTN
                     SVGAGLPGPTTPASWLGRAAAIAGLTVALLAPVVGVWVEAPHRRQVVLTVLTGVVVAL
                     TCSMFFIRDRPSYLWAGLVLLGATAACGDLASVPYNAMLRQLSTRETVGRISGLGWAS
                     GYLGSVMLLLLVYTGFISGSGSGPDAVRGLLQVPVHDGLYVRESMLVAAGWLAVLALP
                     LLLVAHGLPDSGEVHRPTSMLSGYRKLWTEISAEWRRDRNLVYFLGASALFRDGLAAT
                     FAFGAVLGVNVYGLSQGDVLVFGVAACVVAAVGAVLGGFVDHRIGPKPVIVGSLTAIV
                     AAALTLMMLSGSRAFWVCGLLLCLFIGPSQSSARALLLRMAKDGREGVAFGLYTMTGR
                     AVAFLAPWMFSVFVDSFGAVRAGLGGICLVLVAGLVAMLMVRVPVHGAAVADQPSQA"
     gene            7652..>8276
                     /locus_tag="VFI55_08460"
     CDS             7652..>8276
                     /locus_tag="VFI55_08460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7074094.1"
                     /translation="MPIPAPSPDARAVVTGASQNIGEALATELAARGHNLIVTARRED
                     LLNDLATRLAEKYHVAVEVRPADLADPGDRAKLCDELAARPISILCANAGTATFGPVA
                     SLDPAEEKAQVQLNAIAVHDLTLAVLPGMIERKAGGILISGSAAGNSPIPYNATYAAT
                     KAFANTFSESLRGELRGSGVHVTLLAPGPVRTDLPEDAEASLVERLVP"
BASE COUNT         1426 a         2975 c         2610 g         1265 t
ORIGIN      
        1 cggtcggttc cgtacgcgat caccttgctc agcatcgggt cgtagtcgct gccgaccagc
       61 gtgccgggca gcagcgatga gtccacccgc acgccgtcgc cagacggctc gaaaacctgc
      121 agcacctgac cgccggtggg cagaaatcca cgtgccggat cctcggcgta cacccgggcc
      181 tcgatcgcgt gcccgcgcaa ttcgatgtcg tcctgggcga acgccagttt ctcgccggcg
      241 gccacccgga gctgccactc caccaggtcc agtccggtaa ccgcttcggt caccggatgt
      301 tcaacctgca ggcgggtatt catctccatg aagaagaact cgtccgggag ggcggcggac
      361 acgatgaatt ccacggtgcc cgccccgacg tagtcgacgc tgcgggcggt gttgcaggcc
      421 gccgcaccga tccgcgcccg agtctgggga tcaagcagcg ctgagggcgc ctcttcgatg
      481 accttctggt ggcgccgctg caggctgcat tcacgctcgc ccagatgcac cacgttgccg
      541 tgcgtgtcgg cgagcacctg cacctcgata tgcctgggcc gcaacacaaa ccgttccagg
      601 aacagcgtgt cgtcaccgaa tgaggacgcg gcctcccgcc gtgcgctcac cagtgcctcg
      661 cgcagccggg ccggctcgtc caccatccgc atgcccttgc cgccgccgcc cgccgacggc
      721 ttgatcagca ccgggtaacc gacttcagcg gccgccgcga ccaagtcgtc gtcggtcaat
      781 cccgccttcg ccacaccggg caccacgggc acgtcgaagg cgcagaccgc attcttggcg
      841 gtgatcttgt cgcccatcac ctctatcgcc tgtgccggcg gccccaggaa caccaccccg
      901 atgcgttcgc acgcggcagc gaaatcggca ttctccgaga ggaacccgta gcccgggtgg
      961 atcgcctgcg caccggtgcg caccgcggca tcaagcaccc tgccgatgtc caggtagctc
     1021 tcgcgggccg cggcgggtcc cagccgcacc gcttcgtcgg cctcgatcac atgccgcgcc
     1081 gtgacgtcgg cgtcgctgta gacagccacc gaacggatcc ccagccgacg caaggtccgg
     1141 atcacgcgca ccgcgatctc gccgcggttg gctactaaga cggtgtcaaa cactgcactc
     1201 acatccggaa gacgccgtag gagacggagt ccagcggcgc gtgcgcgcac accgaaaggg
     1261 caagcccgac aaacgttctg gtgtcggccg ggtcgatgat gccgtcgtcc cacaaccggg
     1321 ccgtcgaata gtacgggttg ccctggttct cgtactgctc gcggatgggc gccttgaacg
     1381 cctcctcctc gtcggccgac cagggtgagc cggacgcggc cagctgctca ccgcgaacgg
     1441 tggccagcac cgacgcggcc tgctcgccgc ccatcaccga gatccgcgca ttcggccaca
     1501 tccacaggaa gcggggcgaa tatgcccggc cgcacatcga atagttgccg gcgccatagg
     1561 atccgccgat cacaacggtc agcttgggca ctcgtgcgca ggccaccgca gtgaccatct
     1621 tggcgccgtg cttggcgatg ccgccggcct cgtagtcgcg tccgaccatg aagccggcga
     1681 tgttctgtag gaacagcagc ggtatcttgc gcttgtcgca cagctcgatg aaatgtgcgc
     1741 ccttcaaagc agattcgctg aacagcacgc cgttgttggc gactatcccg acggggtggc
     1801 cgtggatgcg cgcaaaggcg gtcaccaggg tcttgccgta tttggccttg aattcggtga
     1861 attcgccgcc gtcgaccagc cgcacgatga cctcgtgcac gtcgtagggc gtccgcggat
     1921 ccggcggcac gacgtcgtag agctccgtct gcgggtgcgt gggctcgacc gaacgccgca
     1981 cttcccactg gctgggctcg cgcggcccga aggtggctgc gatcgagcgg acgatgcgca
     2041 gcgcgtcctc gtcgtcgtcg gcgaggtggt cggtgacacc ggagacccgg gagtgcaggt
     2101 cgccgccccc gagctcttcg gcagtgacga tctctccggt cgcggccttg accagtggcg
     2161 ggccgccgag aaagatcgtg ccctgttcgc gcacgatgac ggcctcatcg ctcatcgccg
     2221 gcacgtaggc gccgcccgcc gtgcacgacc cgagcaccgc cgccacttgc ggaattccct
     2281 tggcgctcat ggtcgcctgg ttgtagaaga tccgcccgaa atgttcgcga tcgggaaaga
     2341 cctcgtcttg gcgcggcagg aaggcgcccc cggagtcgac caggtagatg cacggcagga
     2401 gattctgcag cgcgacttcc tgcgcacgca ggtgtttctt gaccgtcatc gggtaatagg
     2461 tgccgccctt gaccgtggcg tcgttggcga cgatcacgca ctcgcgctcc gacactcggc
     2521 cgatgccggt gatgatgccg gctccgggag actcgtcgcc gtacatgccg cccgcggcca
     2581 gcggagccag ctccaagaac gggctgcccg gatcgaggag gcggtccacc cgttcacgcg
     2641 gcagcagctt gccgcggctg acgtgccgtt cgcgcgcgcg ttcgttgccg cccagagccg
     2701 ccgccgccag cttggcgttc aattcaccaa ccagccggcg gtgctgatcg gcgaatgacg
     2761 tcgcctgatc gaggtttgcc accaccgtgt ccatcgccgc agcagcctcc ttgccgtccg
     2821 gggcgctcca catttcggtt aattttgatt aactgaggtg agggtaccat cgcgcatgcc
     2881 gccgcgtcca gcgaggcatg ggcactcaat ctcggccacg gaggtactcg atcgcccgtt
     2941 gtcgcatctc ggttttgcgc accttgccgg tgacggtcat cgggaaatcg tcgacgatcc
     3001 acagatagcg cgggaccttg aacctggcga tccggcccgc gcaaaaatcg cgcaagccct
     3061 cgatcgtcag tttcggcgcc ccgtcccgca gcatgaccac cgccatgagt tcctccccgt
     3121 accgagcgtc gggcaccccg atgacgtggc cgtcgacgat gtcggggtgg gtgtgcagga
     3181 attcctcgat ctcgcgcggc gagatgttct caccgccgcg aacgacgatg tccttgatcc
     3241 gcccggtgat ctcgatgtac ccgcacgcgt ccatcacggc cagatcgccg gtatgcatcc
     3301 acccgtccgc gtcgacgacc tgcgccgtct gctctgggtc gttccaatag ccggccatca
     3361 ccgaatagcc ccgcgtgcag aactcgccgg ccacgccgcg cggcacggtc tggccggtcg
     3421 tcgagtccgc caccttgatc tccagatgcg gacccgcccg gccgaccgtt ccgacgcgcc
     3481 gttccagcga gtcatccgtg cgcgtctggg tcgagaccgg gctcgtttcg gtcatcccgt
     3541 aacagatcga cacgcccggc atgtgcatgc gctcgatcac cttgcgcatc acctcgaccg
     3601 gacacgggga gccggccatg atcccggtgc gcaggctgct caattcgtag tcggcgaatt
     3661 ccggcaggcc gagctcggcg atgaacatcg tcgggacgcc gtagaggctg gtgcaccgtt
     3721 cggcctgcac cgcgcgcagg gtggcggccg gctcgaaggc gggcgacggg atcaccatgc
     3781 acgcaccgtg gctggtggcc gcgagatttc ccatcaccat gccgaagcag tgatagaagg
     3841 gcaccgggat gcagatccga tcctgcgcgg tgtactcgag cagttcgccc accaggtagc
     3901 cgttgttgag gatgttgcgg tggctcagcg tggctgcctt cgggtatccc gttgtgccgg
     3961 aagtgtattg gatgttgatc ggatcgtggc gatccagcgt cgcggcgatc tcgtccagca
     4021 ggggtgggtc gacttgagta cccgccaact cgtcccagcg gtcgctgtcc aaaagcacga
     4081 cgtcgcgcag gccgaggcaa cgcggcgcga cctcgcccaa catcgcggca tagtccgtgg
     4141 tcttgaatcg cggcgcggcg atcaccatcg agaccccgga ttgtcttagc gcatactcca
     4201 attcgctcgc gcggtacgcg gggttaacgg tcaccaggat cgcgccgatc tccgcggtcg
     4261 cgtactggac gagcacccac tcccatcgat tcggcgacca gatgcccacc cgatcgccca
     4321 cgccgatgcc cgcgcacacc aaccccgtcg ccaatcggcg cacggccgcc cgcagttcgg
     4381 cgtacgtcca ccggcgctcc gcggcaacgt cgacgagcgc gtcccgttgc gcatacgtgg
     4441 cggcggtgtc ggcgaagttg gcgccgatcg tcgtctcgag cagggcgggc agggtcggac
     4501 cgcgatcaaa ggagagcgga ttgtctgcgg ctccggtggc tgccacggtt cctccggctg
     4561 ggttggatac caacttagac cgtcgccgac gcctgctgcc accgcgcgat cgaaagctgg
     4621 cggcgatcgg cccgtgctac gttagtgacg attaaccgac cggacgagtt gtggacggag
     4681 ggccgtcatg gcaacgtccg gcccggcgag tcagcccggc cgtcccgacg cgccgaaccg
     4741 tcgcagccag ttgaaatccg accgacgctt gcagctgctg tcggccgccg aacggctctt
     4801 cgcggagcgc ggtttcctgg ccgtgcgcct ggaagacatc ggcgccgccg ccggcgtcag
     4861 cggtccggcc atctaccgcc acttccccaa caaggagtcg ttgttggtcg agttgctggt
     4921 ggggatcagc acccggctgc tcacgggcgc ccgcgacgta aaggcccgca acggcgacgc
     4981 cgccgccgcg ttgaacgacc tcgtcgactt tcacctcgcc tttgccctgg gcgagcctga
     5041 cctgatccgg atccaggacc gcgacctcgc ccacctgccg gagaccgccg cgaagcaggt
     5101 gcgcaaagcg cagcgccagt acgtcgagat ctgggtcggc gtgctgcgcg agctgaaccc
     5161 caagctggcc gaggccgatg cccggttgac ggcgcacgcc gcattcggcc tgctgaattc
     5221 gacgccgcac agcatgaagt cccccgacaa ttcgcgcggc aggccgccca ccgcggcgcg
     5281 gtcacgcgcg gtcatgcgag cgatgacggt cgctgcgctg cgcgcgggag atcacagccc
     5341 ctgagtcgcg ggtcttgagc gaaaacaccg ccaggcttcc ggcccaggta ccctgcaagc
     5401 catgaggtgg gcatgaacga tccacgtcga tccgagcggt ttattccccc ttacccgggg
     5461 tcaggaccgg agggcccgca gggtccccaa tatccgccgc cggttgaccc ggcatacgcc
     5521 gaccaggctc cctacgctcc cacctacgga gggcccatcc cccaatgggc accgagcttt
     5581 aacgaaaccg accagaccag acacctgccg ccgtactggc aacacgacat gcctcactcg
     5641 ggtgggctgc cgccggaggg tttggctccc ccgccgccgc aaggcccgaa gtctccgcgc
     5701 tggttgtgga tcgctgccgg ggcggcggtg ctgctggtcg tcgcgctggt gatcgcgctg
     5761 atactggcca acgatgcgat caagacccaa accgcagtcc cgccgttgcc cgccatgccc
     5821 gagccgagtc caacgacacc gagcccctcg acacgcacgc cacccctgcc gataccaccc
     5881 ctcttccccc tgccgacgcc gccgaccaac ggatcaggca cccccactca gaccaccggc
     5941 ccggccgcga tgcagagcgt cgtctacaac gtcaccggcg aaggtcgcgc gatcagcatc
     6001 atgtacatcg acaccggcga cgtcatcgag accgagttca acgtcgcgct gccgtggagc
     6061 aaagaggtca gcctgtcgaa gtccgctctt cacccggcaa acgtcacgat cgtcaacatc
     6121 ggtcacaacg tcacctgttc ggtcaccgtc ggcggggtcc aggtaagcca gcgcgtcggg
     6181 gtgggtctga cgatctgcga cgcccgaggc taagcctgcg acggttggtc ggcgaccgcg
     6241 gcgccgtgca ccggaacacg gaccatcagc atcgccacca gtcccgcgac caaaaccagg
     6301 cagatcccgc cgaggccggc acgcacggcg ccgaagctgt cgacgaagac ggagaacatc
     6361 cacggcgcca ggaaggcgac cgcccgtccc gtcatcgtgt agaggccgaa cgcgacgcct
     6421 tccctgccgt cctttgccat ccgcaacaac agggcccgcg ccgacgactg cgacggcccg
     6481 atgaacagac acagcagcag tccgcacacc cagaacgccc gcgatccgga gagcatcatc
     6541 agggtgaggg ccgcggcgac gatcgcggtc aatgacccca cgatgacggg cttgggcccg
     6601 atgcggtggt cgacgaagcc gcccagcacc gcacccacgg cggcgaccac gcacgccgcc
     6661 acgccgaaga ccagcacatc gccttgcgac aggccgtaca cgttgacccc gaggacggcg
     6721 ccgaaggcga acgtcgccgc cagcccatcc cggaaaagcg cgctggcgcc gaggaagtag
     6781 accaggttgc ggtcgcgccg ccattccgcc gagatctcgg tccacagctt gcgatagcca
     6841 ctcagcatgc tcgtgggccg gtggacctct ccagaatcgg gaagcccgtg tgcgaccaac
     6901 agcaacggca aggccagcac cgccagccat ccggcggcca ccagcatcga ctcccggaca
     6961 tagagcccgt cgtgcaccgg cacctgaagc aggccacgca cggcatcggg gccggagccg
     7021 gatccggaga tgaaccccgt gtagaccaac agcagcagca tgacgctgcc gaggtaaccc
     7081 gacgcccaac ccaaaccgga gatccggccg accgtctccc gtgtcgagag ctggcgcagc
     7141 atcgcgttat acgggacgct ggccaggtcg ccgcatgccg ccgtcgcccc cagcaaaacc
     7201 agcccggccc acaggtagct agggcgatcg cggatgaaga acatcgaaca ggtcagcgcc
     7261 accacaactc cggtcaacac ggtcaggacc acctgcctgc ggtgcggggc ctccacccac
     7321 accccgacaa ccggcgccag cagcgccacc gtcaaccccg cgatggccgc ggcgcggccc
     7381 agccaactcg ccggcgtggt cgggccgggc aggcccgccc ccacgctgtt ggttaggtag
     7441 acggagaaca caaaggtggc cacgatcgcg ttcagtccgg tagatccgca gtcccacagc
     7501 gcccacgccg ccacccgcga tcgcgccact gtggccgaac ccgaccccgg ctgactcatg
     7561 cccggcactc tatgtgtata gcggcatgtc tgcggcggcc gacccgccgc gcccggctcc
     7621 gccgcgctcg cgggctgtct acgattggcg gatgccgata cctgctccca gccccgacgc
     7681 gcgcgccgtt gtcaccggag cttcgcagaa catcggcgaa gcgctcgcca ccgaacttgc
     7741 cgcgcgcgga cacaacctga tcgtcaccgc acggcgcgag gacctgctca atgacctggc
     7801 cacgcggctg gcggagaagt atcacgtcgc cgtcgaagtg cgacccgccg atctggccga
     7861 tccgggcgac cgcgccaaac tgtgcgacga actggccgcg cgtcccatct cgatcctgtg
     7921 cgccaacgca ggcaccgcca cgttcggccc ggtcgcgagc cttgacccgg cggaggaaaa
     7981 ggctcaggtg cagctgaatg ccattgcggt gcacgacctt acattggcgg tgctcccggg
     8041 catgatcgag cgcaaggccg gcggcatcct gatttccggt tcagcggcgg gcaattcgcc
     8101 gattccctac aacgccacct atgctgccac gaaagcattc gccaacacct tcagcgagtc
     8161 gttgcgcggc gaattgcgcg gctccggcgt gcatgtcacg ctgttggcgc caggcccggt
     8221 gcgcacggac ctccccgaag acgccgaagc ctcactggtc gaaaggctgg tgcccg
//
LOCUS       DASSGQ010000192         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_560452,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000192 DASSGQ010000000
VERSION     DASSGQ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560452"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VFI55_08465"
     CDS             <1..533
                     /locus_tag="VFI55_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HET7074095.1"
                     /translation="PNDHRDPARAGSVGRPLPGVEVRVVDPATGNDVEAGAVGELWVN
                     TIQNVSEGWLHTGDLARRDCDGYIFPCGRLSDTINRGGEKFGPIEVEEALRSHPAVSD
                     VAVAGITDERLGQRVGAAVVASFPVTLEQLRSHCRELIAYFKLPERLAIVDHIPYNTT
                     GKINRRELATLIARNA"
     gene            549..1262
                     /locus_tag="VFI55_08470"
     CDS             549..1262
                     /locus_tag="VFI55_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074096.1"
                     /translation="MLFLHETHKVVGARQDEFEAAYREGWMPTLAQDDNARLLWYTNH
                     AHGSGASYNVITITGISDGAAWENLVRRAQTGDLRPWMRELDDLRHEVIGKLLLPVAW
                     SPLQVVDLASVPTTGQTHPLTMFMEDTGWPHAPLDDYIQSWDEIYYRPLSQAPSRMRL
                     LDIQACFQVAHGSYRRREAMLWQKIDDSNNYAALVHLLTKEIPPEHRAPGSYMFEALK
                     FRDQWESRLLRTSDWSPLY"
     gene            complement(1746..>2057)
                     /locus_tag="VFI55_08475"
     CDS             complement(1746..>2057)
                     /locus_tag="VFI55_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET7074097.1"
                     /translation="DTRRTRVYDIVRRLDWPTEYNARALGNSFLDTWHGNEAQLAGSL
                     PEAVNAFEKAVAAEDFDTAAVLVGEAVGRIREVRPAADIVADMVGDANRILNRGQAPS
                     R"
BASE COUNT          346 a          666 c          699 g          346 t
ORIGIN      
        1 tgcctaacga tcaccgggat ccggcccgtg ccggctccgt gggccggccg ctacccggag
       61 ttgaggtgcg cgtggtggac cccgccaccg gcaacgacgt cgaggcggga gcggtcggcg
      121 agttgtgggt gaacacgatt cagaatgtca gcgagggctg gctgcatacg ggcgatctcg
      181 cccggcggga ttgcgacggc tatatcttcc cgtgcggccg gctgagtgac accatcaacc
      241 gcgggggaga gaagtttgga ccgatcgagg ttgaggaagc gttgcgttcc catccggcgg
      301 tgagcgacgt ggcggtcgcg ggcatcaccg acgagcggct cggccagcgg gtgggggctg
      361 ccgtggtcgc ctctttcccg gtgacactcg aacagctgcg atcgcactgt cgcgagttga
      421 tcgcctactt caagctgccc gagcggctgg cgatcgtcga ccacatcccc tacaacacga
      481 ccggcaagat caaccgccgt gagctcgcca cgctgatcgc gcgtaacgcc tgataccagg
      541 gggtgccagt gctgtttctt cacgagaccc acaaggtggt cggtgcgcga caagacgaat
      601 tcgaagccgc gtaccgcgag ggctggatgc ccacgctggc ccaggacgac aatgcccggc
      661 tgctctggta caccaaccac gcgcacggca gcggggcgtc ttacaacgtc atcaccatca
      721 ccgggatcag cgacggcgcg gcctgggaga acctggtccg gcgtgcccag accggggacc
      781 ttcggccatg gatgcgcgag ctggacgatc tgcgccacga agtgatcgga aagctactgc
      841 tgccagttgc ctggtcgccg ctgcaggttg tcgacctggc aagcgtgccc accaccggcc
      901 agacgcaccc actgacgatg ttcatggaag acaccggctg gccccacgcg ccgctggacg
      961 actacatcca gtcctgggac gagatctact atcggccgct gtcccaagcg cccagcagga
     1021 tgcggctcct cgatatccag gcgtgcttcc aggtcgcaca cgggagctat cgccgacgag
     1081 aagcgatgct ctggcagaag atcgacgact ccaacaacta tgcggcgctc gttcatctgc
     1141 tcaccaagga gatccctccc gagcaccggg cgcccggcag ttacatgttc gaggcgttga
     1201 agtttcgcga tcagtgggag agccgtctgc tgcggacgtc ggactggtca cccctgtact
     1261 agagcgaaac cgcacgaact ccggggaccg cgccgttcag ctcacggccg agcacgctgc
     1321 gtccacccga acgggctgta gtctcccccg ccaaggtagg ggggtggcac gtaggtcggc
     1381 gcgccgggtg tgacggttgg ggtgccgatg gcgccgggag cggtaccgcc cggcgatggc
     1441 tggactggtt cggcgccgtg gcggcgctgg tcatccggcg cgggcgctgt ggtcgtagtg
     1501 gtcgtggtag ttgttgtgtc tgtcgtggtg gcggggctag ctttgccttg cggaccacac
     1561 gccgcgactg ggaaacacgt ggccagagcg gccatcaggc cgagcaccac cgaccgccgc
     1621 cgggcagcgt tccacgccgt ctgttgtggt gaattgggca cgcaacctga ccctaagccc
     1681 catttctgtg tgatacctgt gaaaatactg catgtcggct agccgtgccg ccggcagcgc
     1741 gaacgtcagc ggctcggcgc ctgcccccgg ttcaggattc ggttggcatc gccgaccatg
     1801 tccgcgacga tgtcagcggc gggacggacc tcgcgaatgc gccccaccgc ctcaccgacg
     1861 agcaccgcgg cggtgtcgaa atcctctgcc gcgaccgcct tttcgaaggc gttcaccgct
     1921 tcgggcagcg agccggccag ttgtgcctcg ttgccgtgcc aggtgtcgag gaaggagttg
     1981 cccagcgccc gcgcgttgta ttcggtgggc caatccagcc gccgcacgat gtcatagacc
     2041 cgggtgcgac gggtgtc
//
LOCUS       DASSGQ010000193         3339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1502558,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000193 DASSGQ010000000
VERSION     DASSGQ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502558"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VFI55_08480"
     CDS             <1..482
                     /locus_tag="VFI55_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074098.1"
                     /translation="MSPYPPPPAPSQPPGRRRGLAIGAGILAGAAAGAVIATAVGATA
                     SSTTGNGLTASSISAAAPDLGPGRGDHMPWTSGTVTAVGSNSVTIKTSSGTSTYGVTS
                     ASDIDKNGEAQLRDLKVGDAVRFTAETANGVTIIDKLHAGDEAKDRPAAPPSGSTGG"
     gene            646..2037
                     /locus_tag="VFI55_08485"
     CDS             646..2037
                     /locus_tag="VFI55_08485"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase family protein"
                     /protein_id="HET7074099.1"
                     /translation="MTVAARDRRPTRPAASAASSTMPSTQPAASQAIVLAVIAAGALA
                     TIALWWNDTTAIHGFGDWLTNAGRVTGLLAGYGVVVLLALMARIPPLERGIGADQLAR
                     WHAHGGRYVVSLVVAHGLLIIWGYAVTAHTNVVSQTATLLTSYPDVLMASVAGLLLVG
                     IGIVSARAARRRMRYETWYYLHLYTYLAVALAFSHQFATGADFITNRPARVAWSALYI
                     AVAAAIAWYRLTVPVWQAIRHQMRVVAVYPEAPGVTSIVISGQHLDELQAQSGQFFRW
                     RFLTRDLWWASNPYSLSAAPRPDWLRITVKDFGEHSSALARLRPGSRVFAEGPYGAMT
                     AIRRRRRKVLLIAGGIGITPLRALFETLPGGKRDITLIYRAGHNADTVFRDELVAIAH
                     QRGAQLHIVTGHRSELGYDPLSKEALMANVPDLRSHDVYVCGPDPMAESVVAALREVD
                     VPRRQIHHESFEF"
     gene            2065..2529
                     /locus_tag="VFI55_08490"
     CDS             2065..2529
                     /locus_tag="VFI55_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018162156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HET7074100.1"
                     /translation="MRRVAFAIVGTIAGLVGLLSFKTHSASVANPPAAVSTTGPSTSG
                     AAPNTGSEPPASSAPGSSSNTVTGDPADTRFGPVQVRITVGNGKLSDVTAVEYPSENP
                     RDQEINSYAIPALNQEAVSAGNAKIDMISGATYTSQGYIQSLQSALDKAGLK"
     gene            2526..3317
                     /locus_tag="VFI55_08495"
     CDS             2526..3317
                     /locus_tag="VFI55_08495"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET7074101.1"
                     /translation="MTPSTARVLAEQQRTVHVEHCMGTVFTIDIRDPGNWDHGIRAVV
                     SWLHEVDRVFSTYRHDSDICRLQRGEITLAAAHPEVAEVLELCADAQVATGGYFTAMP
                     NGRLDPTGVVKGWAIDHASRRLTAHGAANHAVNGGGDMQLVGEAASGRPWTVGISDPR
                     ESRRVLTVVSGRDFAVATSGTAERGAHIVDPFTGHPATRAMSATVTGPSLTQADAYAT
                     AMLVMGPAGVRWIADVDGYEALVVAADATCYRSSGWRIPTEVRSG"
BASE COUNT          569 a         1135 c         1067 g          568 t
ORIGIN      
        1 gcatgtcgcc gtacccgccg cctcccgccc cttcgcagcc gcccggtcgg cggcgtggcc
       61 tggcgatcgg agcgggcatc ctcgccggcg ccgcggccgg agctgtgatt gcgaccgctg
      121 tgggcgcgac cgcatcgtcg acgacgggta atgggctgac ggcgagcagc atctccgccg
      181 ccgcgcctga cctcggccct gggcgcggtg atcacatgcc ctggaccagt ggaaccgtca
      241 ccgcggttgg cagcaactcc gtgacgatca agacaagcag cggaacgagt acgtacggtg
      301 tcacttcggc cagcgatatc gacaaaaacg gtgaggcaca acttcgcgat ctcaaggttg
      361 gcgacgctgt caggttcacg gccgaaacgg cgaacggcgt cacgatcatc gacaagcttc
      421 atgccggcga cgaggccaag gaccgtcccg cggcgccgcc ctccggcagc accggtggtt
      481 gacctattgc caccaaacgc gggaatgaag gcaccttgtc cgcccgggtg agcgactgca
      541 tctggaccgc gctttcagca agcgtgcagg tttcgaccag acgaatctca tgtcgataag
      601 ggataaaggg aggatgcggc ggcaacccac ggtgaggctc gccttatgac cgtcgctgcg
      661 cgagatcgtc ggcccacccg ccccgccgca tcggcggcgt cttccacgat gccatcgacg
      721 cagccggccg cgagccaagc catcgtgctg gcggtcatcg cggccggcgc cttggcgacg
      781 atcgccttgt ggtggaacga caccacggca atccatggat tcggtgactg gctgaccaac
      841 gccggtcggg tgaccggcct gcttgccggt tacggtgtcg tggtccttct cgcgctcatg
      901 gcccggatcc caccgctgga acgcggcatc ggcgcggatc agctggcgcg atggcacgct
      961 cacggcggcc gatacgttgt gagcctcgtc gtcgcgcacg gactgctgat catctggggc
     1021 tacgcggtca ccgcccacac caacgtagtg agccagaccg ccacgctgtt gaccagctat
     1081 ccagacgtgc tgatggcgtc cgtcgccggg ctgctgctcg tcggcatcgg catcgtgtcc
     1141 gcccgggcgg cgcgccgccg aatgcgctac gagacctggt actacttgca cctctacacg
     1201 tacctggccg tcgcccttgc cttcagtcat caattcgcga ccggcgcgga tttcatcacc
     1261 aatcggcctg cccgcgtcgc ttggtccgcc ctgtacatcg cagtcgccgc cgccatcgcc
     1321 tggtaccgac tcaccgtgcc cgtctggcag gccatccgtc accagatgcg ggtggtggcc
     1381 gtgtacccgg aagcgcctgg cgtgacgtcc atcgtgatca gcggccagca tttggacgag
     1441 ctgcaggcgc aatccggtca gttcttccgc tggcgtttcc tcacccgtga cctatggtgg
     1501 gcgtccaacc cgtactcgct gtccgcggca ccgcgcccgg attggctgcg aatcacggtc
     1561 aaggacttcg gcgagcacag ctccgcgctt gctcgcctgc ggcccggcag ccgcgtgttc
     1621 gccgaaggcc cttacggggc gatgaccgcg atccgtcggc gccgccgcaa ggtgctgctc
     1681 atcgcaggcg ggatcggcat aaccccgcta agagcgctgt tcgaaacgct gccgggtggc
     1741 aagcgcgaca ttaccttgat ctatcgcgcc ggacacaacg ccgacaccgt cttccgcgac
     1801 gaactcgtcg cgatcgctca ccagcgtggg gcgcagctcc acatcgtcac cggacatcga
     1861 agcgagctgg gctacgaccc gttgtccaaa gaggcgctca tggccaacgt ccctgacctt
     1921 cgcagccacg acgtgtacgt ctgcggcccg gatcctatgg cggagtcggt cgttgctgcg
     1981 cttcgcgaag tagacgtgcc gcgtcgacaa atccaccacg agtcgttcga gttctgagtt
     2041 cctggaagaa ggagacacat cgtcatgcgt cgggtggcat tcgccatcgt cggcacgatc
     2101 gccggtctgg tcggtctgct gtcgttcaag acgcactcgg catcagttgc aaacccgccg
     2161 gcggcggtga gcaccaccgg tccgagcaca tcaggcgcgg cgccgaacac cgggagcgag
     2221 ccgccggcct cgagcgctcc cggctcgtcg tcgaatacgg ttaccggaga tcccgccgac
     2281 acgcggtttg gtcctgtcca ggttcgtatc accgtgggca acggaaagct ctccgacgtc
     2341 acggcggttg agtaccccag cgagaatccc cgtgaccagg agatcaactc ctatgcgatc
     2401 ccggccctga accaagaagc ggtgtccgcg ggcaatgcga agatcgacat gatctccggg
     2461 gccacttaca ccagccaggg ttatatccag tccctacaga gcgcgctcga caaggccggc
     2521 ctgaaatgac accttcgacc gcacgtgtgt tggccgagca gcagcgcacg gtgcatgtcg
     2581 agcattgcat gggcaccgtt ttcaccatcg acatccgtga cccgggaaat tgggaccatg
     2641 gcatccgcgc tgtggtcagc tggctgcacg aggtcgaccg cgtgttcagc acctaccggc
     2701 acgacagcga catctgccgg ctgcagcgtg gtgagatcac gttggcggcg gcccatccag
     2761 aggttgctga agtgctcgag ctctgtgccg atgcccaagt ggccactggc ggctatttca
     2821 ccgccatgcc gaacggtcga ctcgacccca ccggagtggt gaagggctgg gccatcgacc
     2881 acgcgagccg acgcctgacc gcgcacggag ctgccaacca tgccgtaaac ggcgggggcg
     2941 atatgcagct cgtcggcgag gcggcttctg gacggccctg gacagtcggc ataagcgacc
     3001 cacgcgaatc caggcgagtt cttacggttg tgtccggccg agatttcgcc gttgcgacgt
     3061 cagggacggc cgagcgtggc gcccacatcg tcgacccgtt caccggccac ccggcgacgc
     3121 gcgccatgag cgctaccgtc acgggtccgt cgctgaccca ggctgatgca tatgcgacgg
     3181 ccatgctcgt catgggtcca gctggagtgc gctggatagc cgacgtcgac gggtatgaag
     3241 cgctggtcgt tgcagcggac gccacctgct atcgcagctc gggttggcgc atcccgaccg
     3301 aggttcgctc cggctgagcc gacgttcgct ccggccgag
//
LOCUS       DASSGQ010000194         5262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1290116,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000194 DASSGQ010000000
VERSION     DASSGQ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290116"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFI55_08500"
     CDS             <1..204
                     /locus_tag="VFI55_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074102.1"
                     /translation="VTVSEPIVGSVLGVTVLGETLNTNAVGMVALVVSVAVMIAAAVI
                     LARGQAAGAPATGESPSPATAPS"
     gene            461..1801
                     /locus_tag="VFI55_08505"
     CDS             461..1801
                     /locus_tag="VFI55_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7074103.1"
                     /translation="MSTSDTAAEHDEGRPVIHLSQLLRAPVVARSGETVGRVEDVIVQ
                     LHDTDEYPPVTGIVAGVGGRRVFVGIKSIHEFAADRIILAKNKIDLRGFERREGEVLL
                     RIDVLGHRLIDVAAVELVRAYDVELEDTDAGWVLARLDTRRPPRLFGLIKHSGGHRAR
                     DWKAFEPLIGHARSDAVRRLSDRFGDFKAAEIADLLEEADKVEGGEILDRVHSDPELE
                     ADVFEELDPDKASRLLDGMPDDEVAALLGRMRADDAADAITDLRQSRRRRVLDLMPAP
                     QRTKVITLMGFNPQSAGGLMNVDFVSCATTATAGSALALIGAARTIQPEALIKMHVLD
                     EGKRLAGVVSVVTLLQAEPSKSVGALMDSDPVCISADADLTDIALLMADFNLYSIPVV
                     DEQDRVLGVVTVDDILEATIPEDWRRREPAARQLREITQPEGDRSTSVGGGEAP"
     gene            1861..3474
                     /locus_tag="VFI55_08510"
     CDS             1861..3474
                     /locus_tag="VFI55_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET7074104.1"
                     /translation="MPRTAVLDSAHVGDIEGAFGRISVDDTDRPRTWKARLLTLLAIV
                     GPGIIVMVGDNDAGGVATYAQAGQNYGYSLLWVLLLLIPVLIVNQEMVVRLGAVTGVG
                     HARLINERFGRGWGWFSVGDLFLLNFLTIVTEFIGISLAAEYIGVSKYIAVPISAAAL
                     VAIMASGSFRRWERAMFVFIAITLVQIPMLLMSHPEWGHAAKSFVVPSISGGVSSDAV
                     LLIIAIVGTTVAPWQLFFQQSNIVDKRITPRFMAYERADTVLGAFVVVIGAAALLMTG
                     DWAARSTDTVGKFIDAGDIAHLLGQHSGVLGSMFAIALLDASIIGAAAVTLATSYAFG
                     DVFGLKHSLHRGFADAKQFYLSYTAMVALAAAIVLIPGAPLGLITTAVQALAGLLLPS
                     ASVFLLLLCNDRQVLGPWVNRPWLNWVAGLIVGTLLLLSGVLMATTLFPHINVVMVSS
                     YLALALVVLGLAAGPVLRWMARRQPAPPVAKPPARPVDRSTWRMPPLTLLEPVTWSPG
                     TRLGMIALRTYLVVGAVLLVVKAIQLSRG"
     gene            complement(3566..4867)
                     /locus_tag="VFI55_08515"
     CDS             complement(3566..4867)
                     /locus_tag="VFI55_08515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7074105.1"
                     /translation="MSSTTQRAGRAKVRTPLNRSQIAGFWGAWTGWTLDGMDSFIYAL
                     VLTPALTELLPRSGFAATTSNIGLAGSILFGLFLVGWGLSFIWGPIADRFGRTKVLAA
                     TIFTFAIFTGLAAASQNVWELATFRFIAGVGIGGEWALAGTYVAEAWPEDRRKMGAGY
                     LQTGYYAGFFLAAALNYTIGVHFGWRAMFLTGAVPVVVAILILTRVKETEKWEKAEAD
                     KTVRVKPLREILGPRYRRRTWVAAALVTIAIVGLWAGAVYEPTAVIQLAQHAGFDKSG
                     ATKTASWATALLSVGTILGCLLVPVLAERVGRRRTLAIYFAGMAVSIAGSFGWAFYLP
                     NGLAPFIAWLFVLGFFGGNFALFSLWLPEQFETRVRATAFAFCTSFGRFVGAGVNFLL
                     GAAVLHTHTLGLPVALTAVAFVVGLFIIPLAPETRGELLPE"
     gene            complement(4958..5191)
                     /locus_tag="VFI55_08520"
     CDS             complement(4958..5191)
                     /locus_tag="VFI55_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074106.1"
                     /translation="MAKFRLIDPHGEIVANKDIDSAEAAHRWFANSVGSTELGWRMEV
                     DDDGQWAFFDDTEGFTAVASRRPASATRPPASK"
BASE COUNT          918 a         1763 c         1670 g          911 t
ORIGIN      
        1 gtgaccgttt ccgagcccat cgtcggttcg gtgctcggcg tcaccgtgct cggcgagacg
       61 ctgaacacca acgccgtcgg catggtcgct ctggttgtgt cggtagcggt aatgattgcg
      121 gcggcggtga tcctggctcg cggccaggcc gctggggccc cggccactgg cgagagcccg
      181 agcccggcaa ccgcaccgag ctgacatgta agtgttgacg caccgtcgac gcacggaaat
      241 cgtcgcgccc gattttcccc actggctgcc cgccgtttac cgattggctc tagtcgttgg
      301 ccgcgacgga ggcgtacgct ctggcggccg aaccggcaag ccacgatact tgcgccatca
      361 gcgaacctgg ttttacgccg tacccgcatc tggcatcgcg acacatggtc tcgtgcagcc
      421 tgctcgcggg gggtctatcg cgcagcgttg aggaggacaa gtgagcacat cagacacagc
      481 ggctgagcac gacgaggggc ggccggtaat ccatctttca cagctgctgc gcgcgccggt
      541 agtggcccgc tccggcgaga cggtgggccg ggtcgaggac gtgatcgtgc agttgcatga
      601 cactgatgaa tacccgccgg tcacggggat cgttgccggg gtcggcggac gtcgcgtgtt
      661 cgtcggcatc aaatccatcc acgaattcgc ggcggaccgg attatcttgg ccaagaacaa
      721 gattgatctt cgcggttttg aacgccgcga aggtgaggtg ttgttgcgca tcgatgtgtt
      781 gggccaccgg ttgatcgacg tggccgccgt ggagctggtg cgcgcatatg acgtcgaact
      841 cgaagacacc gacgccgggt gggtgctcgc acggctggac acccgccgcc caccacgact
      901 attcgggctt atcaagcact ctggcggcca cagagcgcga gattggaaag cattcgaacc
      961 gctcatcggt cacgcgcgct ccgatgccgt gcggcgcctg tccgaccggt tcggcgactt
     1021 caaagccgcc gagatcgccg atcttcttga agaggccgac aaggtcgagg gcggcgaaat
     1081 cctcgaccgg gtgcacagcg acccggaatt ggaagccgac gtgttcgagg aactggatcc
     1141 cgacaaggcc agccgattac tcgacggcat gcccgacgat gaggtggccg ccctgctggg
     1201 ccgaatgcgc gccgacgacg ccgccgacgc aatcaccgac ctgcgccaat cgcgacgccg
     1261 gcgcgtgctg gacctgatgc ccgccccgca gcgcaccaag gtgatcaccc tgatggggtt
     1321 caatccccaa agtgccgggg ggttgatgaa cgtcgacttc gtctcctgcg ccaccaccgc
     1381 gaccgcaggt tcggcgctcg cgctgatcgg ggccgcacgc accatccagc ccgaggcatt
     1441 gatcaagatg cacgttcttg atgagggtaa gcgcctcgcc ggtgtggtgt cggtggtcac
     1501 cctgctgcag gccgagccca gtaaaagcgt tggagcactg atggattccg acccggtctg
     1561 catcagcgcc gacgcggacc tgaccgacat cgcgctgttg atggcggact tcaacctcta
     1621 ctccatcccc gtcgtcgacg agcaggaccg cgtgctcggg gtggtcacgg tcgacgacat
     1681 cctcgaagcg accatccccg aggactggcg tcgccgcgaa cccgccgcgc gtcagttgcg
     1741 cgaaatcacc caacccgagg gtgaccgcag cacatctgtc ggaggaggtg aggcaccgtg
     1801 acctcaggcg ccgacaacgc cgcccacccg gaggcgccgg ccgacagagc ccgcccggcg
     1861 gtgccgcgta ccgcggtgct cgacagcgca catgtgggtg atatcgaggg cgcgttcggg
     1921 cgtatcagcg tcgacgacac ggaccgtccc cgcacgtgga aggcacggct gctgaccctg
     1981 ctggccatcg tcggtcccgg aatcatcgtg atggtcggcg acaacgacgc cggcggggtg
     2041 gccacctacg cccaggccgg ccagaactac ggctactcgt tgctgtgggt gctgttgctg
     2101 ctgatcccgg tgctcatcgt caaccaggaa atggtggtgc ggctgggcgc ggtcaccggg
     2161 gtcgggcacg cccggctgat caacgagcgc ttcggccgcg gctggggctg gttttccgtc
     2221 ggcgacctgt tcctgctcaa cttcctgacg atcgtcaccg agttcatcgg catcagcctc
     2281 gccgccgagt acatcggcgt ctccaaatat attgcggtgc cgatctcggc ggccgcgttg
     2341 gtcgcgatca tggcgagcgg cagcttcagg cggtgggagc gggccatgtt cgtcttcatc
     2401 gccatcaccc tggtgcagat cccaatgctg ttgatgtcgc atccagaatg gggtcatgcg
     2461 gcgaagtcct ttgtggtgcc aagcatctcg ggcggtgtga gctcggatgc ggtgttgttg
     2521 atcatcgcca tcgtcggcac cacggtggcg ccctggcagc tgtttttcca acagtccaac
     2581 atcgtcgaca agcgcatcac cccgcgattc atggcctatg aacgcgccga cacggtgctg
     2641 ggggcctttg tcgtggtgat cggtgccgcc gcgctgctga tgaccggcga ctgggccgct
     2701 cggtccaccg atacagtggg caaattcatc gacgccggcg acatcgcaca cctgctcggc
     2761 cagcacagcg gggtgctcgg ctcgatgttt gcgatcgcgc tgctggacgc ctccatcatc
     2821 ggcgcggccg ctgtgaccct ggccaccagc tacgccttcg gcgacgtgtt cggcctcaag
     2881 cactcgttgc accgcgggtt cgccgacgcc aagcagtttt acctgtccta caccgccatg
     2941 gtcgcgttgg ccgcggctat cgtgttgatc cccggcgcgc cgcttgggct gatcaccacc
     3001 gcggtgcagg cccttgccgg cctgctctta cccagcgcca gcgtgtttct cctgttgttg
     3061 tgcaacgacc ggcaagtgtt ggggccctgg gtcaatcgtc cctggctcaa ttgggtcgcc
     3121 gggcttatcg tcggtaccct gctgctgctg tcgggggtcc tgatggccac cacgttgttc
     3181 ccgcacatca acgtcgtcat ggtctccagc tacctggcgc tggccctggt ggttctcggg
     3241 ctggcggcgg ggccggtgct gcgatggatg gcccgacgtc aacctgcgcc gccggtcgcg
     3301 aaaccgcccg cgcggcccgt ggaccgcagc acgtggcgga tgccacccct gaccctgctc
     3361 gaaccggtga cctggtcgcc agggacccgc ctcggcatga tcgccttgcg cacttacctg
     3421 gtcgtcggtg cggtgctgct ggtggtcaaa gccatccagc tcagccgcgg ctagccgagg
     3481 atctgtcgag ggcagcgcga tatcgaaaga cgttgctgct cagtcgatgg agcgactcgc
     3541 cgcacgacac gagtggtgca ctcccctatt ccggcagcaa ctcaccgcgc gtctcgggtg
     3601 ccagcggaat gatgaaaagc ccgaccacga aagcgaccgc cgtcagcgca acgggcaaac
     3661 ccaaagtatg cgtgtgcaac accgccgcgc cgagcaggaa gttcacgccg gccccgacaa
     3721 agcggccgaa cgacgtgcag aacgcgaagg ccgtcgcgcg cacccgcgtc tcgaactgct
     3781 cgggtaacca gaggctgaac agcgcgaagt tgccgccgaa gaaaccgagc acgaagagcc
     3841 acgcgatgaa cggcgcaagc ccattgggca gatagaacgc ccagccaaag cttccggcaa
     3901 tcgaaaccgc catccccgca aaataaatcg cgagtgtcct tcggcggccc acgcgctccg
     3961 cgagaaccgg caccaaaaga caccccaaga tggtgccgac agacaacagc gcagtggccc
     4021 atgacgcggt tttggtcgcg ccgctcttgt cgaaacccgc gtgctgcgcc aactgaatca
     4081 cggccgtcgg ttcatatacc gcgcccgccc acaaacccac gatcgcaatc gtcaccagcg
     4141 cggcagccac ccaggtgcgg cgccggtagc gcggcccgag tatctcgcgc aacggcttca
     4201 cgcggaccgt cttgtccgcc tcggccttct cccacttctc ggtttccttc acgcgcgtca
     4261 agatcagaat ggcgacgacg actggcaccg cgccggtcag gaacatcgcg cgccagccga
     4321 agtgcacgcc tatcgtgtaa ttcagcgccg ccgccaaaaa gaagccggcg tagtagccgg
     4381 tctgcaaata gcccgccccc atcttgcgcc ggtcctccgg ccacgcctca gccacgtaag
     4441 tgcccgcgag cgcccactcc ccgccgatgc cgacacccgc gataaagcgg aaagtcgcaa
     4501 gctcccagac gttttgcgac gcagccgcaa ggccggtgaa gatggcgaag gtgaagatcg
     4561 tcgcggccag cactttcgtc cgcccgaagc ggtctgcgat cggcccccag atgaacgaca
     4621 ggccccagcc cacaaggaac aggccgaaca ggatcgatcc cgccagcccg atattgctgg
     4681 ttgtcgcggc aaagcccgaa cgcggtagca gctcggtcag cgcgggtgtg agcacgagcg
     4741 cgtagatgaa cgaatccatc ccgtcgagcg tccagccggt ccaggcgccc cagaatcccg
     4801 cgatctgtga acgattgagc ggcgtgcgaa ctttggcgcg acctgcccgc tgcgtcgttg
     4861 aactcattac gcctccggtt gatccggtcc agcggccgct caacattcag cgccgggctg
     4921 cgggattcgg ccgcgaagcg cctgccccgc cagtcgccta cttggaagct ggcggtcggg
     4981 tagccgatgc cggacgtcgg ctcgccacgg cggtgaagcc ttcggtgtcg tcgaagaacg
     5041 cccactgacc gtcgtcgtcg acttccatcc gccaacccag ctcggtactg ccaacggagt
     5101 tggcaaacca cctgtgcgcg gcctccgcgc tgtcaatgtc cttgttcgcc acgatctcgc
     5161 cgtgtgggtc gataagtcgg aacttggcca tgcgatgtga ttatcccttc cagccgtaat
     5221 tcaatcgcat ttagtggccg ccgactagtg ccttcggtgg tg
//
LOCUS       DASSGQ010000195        21109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_925733,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000195 DASSGQ010000000
VERSION     DASSGQ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 21109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21109
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925733"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /gene="sucB"
                     /locus_tag="VFI55_08525"
     CDS             complement(<1..1227)
                     /gene="sucB"
                     /locus_tag="VFI55_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HET7074107.1"
                     /translation="MAFSVQMPALGESVTEGTVTRWLKQEGDTVELDEPLVEVSTDKV
                     DTEIPSPAAGVLTKIIAQEDDTVEVGGELALIGDASEAGAPAAPAQPSQPEPQAAAPP
                     PPEPEPQPAAPARPEPQPAAPAQQSSGGSATPVLMPELGESVTEGTVTRWLKKVGDSV
                     QVDDPLVEVSTDKVDTEIPSPVAGILLSITAEEDDTVPVGGELARIGAGAQAGAPAPA
                     AAAPPPPPPPPPPPAPKPEPAPQAQPEPKPAPAPAPKPEPAQAPPAAQAEPAAQTDGD
                     GAPYVTPLVRKLATENKIDLAEVTGTGVGGRIRKQDVLAAAEQKAQKEQAAKAPAPAA
                     PAPAAAPASPGKKAPAAATPAEALAHLRGTTQKASRIRQITATKTRESLQATAQLTQT
                     HEVDMTKIVGLRAKSKA"
     gene            1443..3215
                     /locus_tag="VFI55_08530"
     CDS             1443..3215
                     /locus_tag="VFI55_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7074108.1"
                     /translation="MPEIEQLPQHFVDSADGVRIAVYEEGNREGPTVVLVHGFPDSHV
                     LWDGVVPLLAERFRVIRYDNRGVGQSSVPKPVSAYTMARFADDFAAVIGELSPGRPVH
                     VLAHDWGSVGIWEYLSRAGASDRVSSFTSVSGPCQDQLVDYIFGGLRRPWRPRTFARA
                     ASQALRLTYMVFFSVPVLAPLLVRLALSVKPIRRNIVGNIPDELIHHSAKLAGDAAHS
                     IKTYPANYFSAFSRKRPIHVVDVPVQLIVNTKDPYVRPYGYDHTTRWVPRLWRRDIKA
                     GHFSPMSHPRVMAAAVHDFADLAEGKQVSRALLRAQIGRPREDFGDTLVAVTGAGSGI
                     GRATAFAFAREGAEVVVSDIDEAAVKQTAAEIAGRGGVAHPYVLDVSDAEAVETFAER
                     VSAAHGVPDVVVNNAGIGHAGGFLDTPAEQFERVLDVNLGGVVNGCRSFGRRLVERGT
                     GGHIVNVASMAAYAPSQSLSAYCTSKAAAYMFSDCVRAEFDAAGVGLTTICPGVINTN
                     IINTTRIDAPAGKTDVVVDRRGQLGKLFALRHYGPENVAKAILSSVKKNKPIRPVAPE
                     AYAFYGVSRVAPQALRSTARLRVI"
     gene            complement(3226..3570)
                     /locus_tag="VFI55_08535"
     CDS             complement(3226..3570)
                     /locus_tag="VFI55_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET7074109.1"
                     /translation="MTVRRLIIAMGAALLLAGVIGLLTPVSISDGNGNSIACGNAVAT
                     DLSAAKSANDKSVANIPILNQLIPHTDYVAQCQSSVGSRRAWTIPLTVVGIVVVAGAL
                     LVRRNEGAATGL"
     gene            complement(3681..5252)
                     /locus_tag="VFI55_08540"
     CDS             complement(3681..5252)
                     /locus_tag="VFI55_08540"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HET7074110.1"
                     /translation="MPNESGYQSPSVNVATALPKRGTANAVLLVPVVSTGDEDRPGAV
                     SVIRAAAEPFLPAEAVAEIEGGLRALGATGGSEQVHRLVVPSLPVASVLTIGLGKPRS
                     EWPAETIRRAAGVAARSLGGAETLITTLTELPGESGGDTCSPAVEGLILGGYRFTAFR
                     SDKTAPKDKGLRKITVLATSKDAKKQSAHGAAVAAAVATARDLVNTPPNHLFPAELAR
                     RAKVLGESVGLEVEVLDDKALQKAGYGGVIGVGQGSSRPPRLVRLIHRGSRLAGPKQA
                     KTAKTVALVGKGVTFDTGGISIKPAASMHYMTSDMAGAAAVIATVTLAAQLQLPIDVI
                     ATVPIAENMPSATAQRPGDVLTQYGGTTVEVLNTDAEGRLILADAIVRACEDDPDYLI
                     ETSTLTGAQTVALGARIPGVMGSDEFRDRVAEISQRVGENGWPMPLPDELKDDLKSTV
                     ADLANVSGQRYAGMLVAGVFLREFVADGVSWAHIDVAGPAYNTGSAWGYTPKGATGVP
                     TRTMFAVLTDIAEKG"
     gene            complement(5273..6409)
                     /locus_tag="VFI55_08545"
     CDS             complement(5273..6409)
                     /locus_tag="VFI55_08545"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7074111.1"
                     /translation="MSDPLDSADSFDSLDIEELEAAGVANARERADLIKYLDDLGFTA
                     DEVVEAERRGRLFGLAGDVVLWSGPPIYTVQAAADELGVSAEDVAQAWALLGLTIAGP
                     DIPALSQADVDGLATWLALKTAVGADGASGMLRVLGAAMARLAEAEASVIRAGMPDIQ
                     MTHTHNELATAQAYRAAAEFIPRLGALMDTVHRHHLISARTYFEGVLQDTSASVTCGV
                     GFADLSSFTALTQTLTPAQLSELLNEFAATVADVVHADGGRVVKFIGDAVMWVSPTAG
                     QLVQAAVDLVDHPRARDEGLQVRAGLAYGTVLAINGDYFGNPVNLAARLVATAAPLQI
                     LASAELHGKLPDWPATALGPLTLKGFDTPVTAYDLHVGPGQGPG"
     gene            6568..7662
                     /gene="gcvT"
                     /locus_tag="VFI55_08550"
     CDS             6568..7662
                     /gene="gcvT"
                     /locus_tag="VFI55_08550"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003914049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HET7074112.1"
                     /translation="MTELQHGPLEDRHRELGASFAEFGGWLMPVSYAGTVTEHNATRT
                     AVGLFDVSHLGKALVRGPGAAQFVNSALTNDLGRVGPGKAQYTLCCTESGGVIDDLIA
                     YYVDDDEIFLVPNAANTAAVVKALSAAAPAGLTITNEHRSYAVLAVQGPRSPEVLGEL
                     GLPTDMDYMGYDDASFSGVPVRVCRTGYTGEHGYELLPPWDSAGVVFDALAAAVSDAG
                     GEPAGLGARDTLRTEMGYPLHGHELSLDISPLQARCGWAVGWKKDAFYGRDALLAEKA
                     AGPRRLLRGLRMIGRGVLRPGLTVLAGDTPVGVTTSGTFSPTLQVGIGLALIDTDAGV
                     DDGQQITVDVRGRPVDCEVVRPPFVDVKTR"
     gene            7719..8825
                     /locus_tag="VFI55_08555"
     CDS             7719..8825
                     /locus_tag="VFI55_08555"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HET7074113.1"
                     /translation="MTSGSLGFTVARAAHPASDAERESILAEPGFGKYFTDHMASIDY
                     AEGQGWHNARVLPYGPIELDPSAIVLHYAQEVFEGLKAYRWVDGSIVSFRPDANAARL
                     RSSARRLAIPELPEGLFIESLRQLVAVDSAWVPRAGGEEALYLRPFIFATEPGLGVRP
                     SKRYRYMLIASPAGAYFKGGISPVTVWVSTEYVRASPGGTGAAKFGGNYAASLLAQAE
                     AAENGCEQVVWLDAVERRFVEEMGGMNLFFVLGSGGTARLVTPELSGSLLPGITRDSL
                     LQLALDAGFSVEERKIDIDEWQKKAAAGEITEVFACGTAAVITPVSRVKYGDTEFTIA
                     DGQPGEVTMALRDTLTGIQRGTFADTHGWMARLG"
     gene            complement(8903..9643)
                     /locus_tag="VFI55_08560"
     CDS             complement(8903..9643)
                     /locus_tag="VFI55_08560"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HET7074114.1"
                     /translation="MIRSLATAFAFATVMPIPRTGNAPMGRGAMTALPVVGAALGALA
                     AAVAWCGGWVFGPSSTLPGLLAVVALLLATRGLHIDGVADTADGLGCYGPPQRALAVM
                     RDGSTGPFGVAAVVVVILLQGLAFSALRPAGIAVAVFAGRVTAVLACRRSVPAADGSA
                     LGARVAGSQPAPVAAAWIAVLLAVSLLAGSRPWQGPTAVLVAVCCGAALVCHCVRRFG
                     GISGDVLGAAIELTTTVSAVALAGLIRF"
     gene            complement(9640..10698)
                     /gene="cobT"
                     /locus_tag="VFI55_08565"
     CDS             complement(9640..10698)
                     /gene="cobT"
                     /locus_tag="VFI55_08565"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HET7074115.1"
                     /translation="MMEFAPVALPDAAAASAARARQDTLTKPRGALGRLEDLSVWVAS
                     CQGHCPPTQFERPRVVVFAGDHGVARSGVSAFPPEVTAQMVANIDAGGAAINVLADVA
                     GATVRVADLAVDADAASERIGAHKVRRGSGDITVQDALTDAETVEAIAAGRAIADEEV
                     DAGADLLIAGDMGIGNTTPAAVLVAALTNTEPVAVVGFGTGIDDAAWARKTAAVRDAL
                     FRSRRVLPDAVGLLRCCGGADLAAMAGFCAQAAVRRTPLLLDGMAVTAAALVAERLAP
                     GARRWWQAGHRSTEPGHGLALAALELEPILDLRMRLGEGTGAAVALPVLRAAVAALLS
                     MATFREAGVVGPSSSTPS"
     gene            complement(10695..11231)
                     /locus_tag="VFI55_08570"
     CDS             complement(10695..11231)
                     /locus_tag="VFI55_08570"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HET7074116.1"
                     /translation="MRTLVLGGIRSGKSRWAEEAVAKSLPPGQPVRYLASGVTAQADA
                     AWEDRVAQHRSRRPAHWSTVETDDITTQLRQSPDTPTLVDDLGGWLTGTLDRNDAWEK
                     GSVAAPVDEMISAVGAFGSTLVLVSPEVGLTVIPATASGRRFADELGSLNQRVAELCD
                     RVVLVVAGQALSLKQPGA"
     gene            complement(11259..11897)
                     /locus_tag="VFI55_08575"
     CDS             complement(11259..11897)
                     /locus_tag="VFI55_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3043 domain-containing protein"
                     /protein_id="HET7074117.1"
                     /translation="MTPTVVDAASSLDAGSRGSGRTGPKGRPTRKRSQATRNPKKGPV
                     APAPMTSSEARARRKSLAGAKLSREERKAEKTVSRARMSERRDRMMAGEEAYLLPRDQ
                     GPVRRYVRDVVDARRNVLGLFMPATVVLLFIMFAVPQLQLYVSPAMLVLMAVMSVDGL
                     ILGRKVNRRVDAKFPKNTESRWKLGLYAASRASQMRRLRAPRPQVERGSSVD"
     gene            12152..13228
                     /locus_tag="VFI55_08580"
     CDS             12152..13228
                     /locus_tag="VFI55_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET7074118.1"
                     /translation="MQVLVAPDCYGDSLSAVEAAAAIATGWTRSRPGDRFLVAPQSDG
                     GPGFVEVLSNRLGQRRRMRVCGPMDTSVEAEWVFDPASATAYLECAQACGLALLDGPP
                     TPETALAAHSRGVGQLIIEALRAGSVRIVVGLGGSACTDGGQGMIAELGGLQAARLRL
                     AEVQVIAASDTEYPMVGPWGAARVFGPQKGADTAAVAALEARLEAWAIELEAVAGRDV
                     SSEPGAAAAGGIGAGLLALGGRCESGAAIIAAHTHLDDDLETAEMIVTGEGRLDEQSL
                     HGKVVGFLADAARPLGIPVIVLAGQVGLDNSAVRSSGIMSALSIADYAGSVRLAQADA
                     ANQLMGLASQVAARLGNSGPARYR"
     gene            13297..13653
                     /locus_tag="VFI55_08585"
     CDS             13297..13653
                     /locus_tag="VFI55_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET7074119.1"
                     /translation="MTVQNESTAKTHGAILTEAAAAKAKSLLDQEGRDDLALRIAVQP
                     GGCAGLRYNLFFDDRTLDGDLTADFGGVTLTVDRMSAPYIEGASIDFVDTIEKQGFTI
                     DNPNATGSCACGDSFN"
     gene            complement(13702..14373)
                     /locus_tag="VFI55_08590"
     CDS             complement(13702..14373)
                     /locus_tag="VFI55_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003411415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074120.1"
                     /translation="MAGPHPPNHTVGTDGPTPHTEAQPLEFPDEPTAGAFRPPPPSDA
                     GLSPANYAGQPPMIYPRRRSKRRLIIGMLVTVALVAAMTVAIVYGVRTNGANTGSSFS
                     EGAAKSAIQGYLDALERRDVDAIVRNALCGMYDGVRDKRADQALAKMSSDAFRKQFSE
                     VELTAVDKIVYLSQYQAQALFTMRVTPAIGGPARGDVQGIAQLLFQRGQIMVCSYVLR
                     TASSY"
     gene            14564..15538
                     /locus_tag="VFI55_08595"
     CDS             14564..15538
                     /locus_tag="VFI55_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HET7074121.1"
                     /translation="MTIAVTGSIATDNLMRFPGRFSEHLLADHLQKVSLSFLVDDLVI
                     HRGGVAGNIAYAIGVLGGDVALVGAAGDDFGDYRGWLQSHGVNCDHVLISESAHTARF
                     VCTTDLDMAQIASFYPGAMSEARNIKLADVASSVGALDLVIIGANDPEAMVVHTEECR
                     ELGLAFAADPSQQLPRLSGDEISKLIDGAAYLFTNDYEWELLLSKTGWSESDVMEKVD
                     LRVTTLGAKGVDIVERDGTTVHVGVVPETSQTDPTGVGDAFRAGFLTGRSGGLSLERS
                     AQLGSLVAVLVLESTGTQEWDWNHEVAVNRLAGAYGEDAAAEIAGVLA"
     gene            complement(15674..17632)
                     /gene="asnB"
                     /locus_tag="VFI55_08600"
     CDS             complement(15674..17632)
                     /gene="asnB"
                     /locus_tag="VFI55_08600"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET7074122.1"
                     /translation="MCGLLAFVAAPAGAGSRGIDAAAADGTISHALRLMRHRGPDEPG
                     GLAAPDADGAVVFGFNRLSFIDIAHSHQPLRWGPPEAPGRYVLVFNGEIYNYLELRDE
                     LATRHGAVFATDGDGEAIVAGYHYWGTDVLTRLRGMFAFALWDTETRELFCARDPFGI
                     KPLFMATGTGGTALASEKKCLMDLAELVGFDTGIDDRAVQHYTVLQYVPEPETLHRGV
                     RRLESGCYARIRPGQQPEVTRYFVPRFNAIPFNRDTEQARYDEITAVLEDSVAKHMRA
                     DVTVGAFLSGGIDSTAIAALAIRHNPRLITFTTGFEREGFSEIDVAVASAEAIGARHI
                     AKVVSPDEFVAALPEIVWYLDEPVADPALVPLFFVAREARKHVKVVLSGEGADELFGG
                     YTIYREPLSLKPFDYLPSPLRRSMGKVSKPLPDGMRGKSLLHRGSLTLEERYYGNARS
                     FSDVQLRDVLPGFRDDWTHTDVTASVYAESAGWDPVARMQHVDLFTWLRGDILVKADK
                     MTMANSLELRVPFLDPEVFAVASRLPVEAKITRTTTKYALRRALEPIVPAHVLHRPKL
                     GFPVPIRHWLRAGPLLEWAYALVDSSQADHLIDLAAVRRMLDEHRSGISDHSRRLWTL
                     LIFMLWHAIFVEHSVVPQISEPVYPVQL"
     gene            17789..18886
                     /locus_tag="VFI55_08605"
     CDS             17789..18886
                     /locus_tag="VFI55_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET7074123.1"
                     /translation="MTPREQVRSQRLSHGRFQRRSGSANRRLPRRLRTLALVATFGLL
                     ALTLSGCSWSEALGLGWPEGITHEAHLNRELWVGAVIASLVVGVLVWGLMFWSAIFHR
                     KKATDTELPRQFGYNMPLELVLTVTPFLIISVLFYFTVVVQEKMLHLAKDPEVVIDIT
                     SFQWNWKFGYQRINFKDGTLTYDGADPARKNAMVSKPEGKDAHGEELVGPVRGFNSQD
                     RTYLNFDKVETLGTSSEIPVLVLPTGKRIEFQMASADVIHNFWVPEFLFKRDVMPNPV
                     ANNSVNVFQVEEITKAGAFVGHCAEFCGTYHSMMNFEVRVVPPNDFKAYLQQRIAGKT
                     NAEALQAIAQSPIAVTTHPFETRRGELTTSQ"
     gene            18900..19319
                     /locus_tag="VFI55_08610"
     CDS             18900..19319
                     /locus_tag="VFI55_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HET7074124.1"
                     /translation="MHIEARLFEFCAVFFILAAVLYGVLTALFATGGEEWAGTTALTL
                     TGGLALIVATFFRFVARRLDTRPEDYEGAEISDGAGELGFFSPHSWWPILVALSGSVA
                     ATAIALWLPWLIVAGVALILASVAGLVFEYYIGPEKH"
     gene            19503..20450
                     /locus_tag="VFI55_08615"
     CDS             19503..20450
                     /locus_tag="VFI55_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmpS family transport accessory protein"
                     /protein_id="HET7074125.1"
                     /translation="MSGPNPPGWEPEEPDSAGEGGHEPESDGSRDELAPSELDSHDDL
                     AHLDDSAAIAAPDRTGQTDAYSRAYSAPESEHFISGSYLPGDLRLYEYDGYEESSESD
                     DEEGAPRWPWVVGVAAILAAIALVVSVSLLVARTDTSKLANPGTTTTPSQPPMQDEIT
                     TTKPPPPPPPPPPPPSTETPTATETQTVTVTPPPPPPPPPPAAPAPPPATSTPAAAPP
                     PSTTAPAGPRQVTYSVTGTKAPGDIISVTYVDASGRRRTQHNVYIPWSMTVTPISQSD
                     VGSVEASSLFRVSRLNCSITTSDGTVLSSNTNDLPQTSC"
     gene            20450..21091
                     /locus_tag="VFI55_08620"
     CDS             20450..21091
                     /locus_tag="VFI55_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2561 family protein"
                     /protein_id="HET7074126.1"
                     /translation="MVSKYSAYRRGSETVPPDVVDRILVGTCAAIWLVLLGVSVAAAV
                     ALVDLGRGFHKMAGNSHTTWLYAVIIVSALIIAGAIPVLLRARRMAPADPAARSVLGS
                     TRPLVRLMSPAPRTAAERARQQRVVRAEEPAGEWSGVDVDRVWLRGTVVLTGTMGAAL
                     IAVATATYLMAVGHEGASWVVYGLAGAITAAMPAVEWLHIRQLRSVVSARVAA"
BASE COUNT         3242 a         7105 c         7302 g         3460 t
ORIGIN      
        1 ggccttggac ttggcgcgca gcccgacgat cttggtcatg tcgacctcgt gggtctgcgt
       61 gagttgggcc gtggcctgca gggattcccg ggtcttggtc gcggtgattt gacggatccg
      121 gctggccttt tgggtggtgc cccgcaggtg cgccagcgcc tctgctgggg tggcggcggc
      181 cggggccttc ttgccggggg aagcaggcgc ggcggcggga gccggcgcgg ccggggcggg
      241 cgctttggcg gcttgttctt tctgagcttt ttgctcagcc gcggccagca cgtcctgctt
      301 gcggatgcga ccgcccaccc cggtgcccgt gacctcggcg aggtcgatct tgttttcggt
      361 ggccagtttt cgcaccagcg gggtcacgta aggcgcgcca tcaccatcgg tctgcgcggc
      421 gggctcggcc tgcgcggcgg gcggggcctg cgccggctcg ggcttcggcg cgggggccgg
      481 tgcgggcttt ggctcgggct gggcttgcgg cgccggttcg ggcttgggtg ccggcggcgg
      541 cggtggagga ggaggcggtg gcggcgccgc ggccgccgga gccggcgcgc cggcctgtgc
      601 accggcgccg atccgcgcca actcgccgcc caccggcacg gtgtcgtctt cctcggcggt
      661 gatgctgagc aggatgccgg ccaccggcga agggatctcg gtgtccacct tgtcggtgga
      721 cacttccacc agcgggtcgt cgacctgcac cgagtctccg accttcttga gccagcgagt
      781 cacggtgccc tcggtcaccg actcgcccag ctcgggcatc agcaccgggg tcgcactgcc
      841 gccggaactc tgttgggccg gcgccgcggg ttggggctcg gggcgggccg gcgcggcggg
      901 ttggggctcc ggctccggtg ggggcggggc cgcggcctgc ggctcgggtt ggcttggctg
      961 ggcgggcgcc gccggcgcgc cggcttcgga ggcgtccccg ataagggcca gctcgccgcc
     1021 gacctcgacg gtgtcgtcct cctgggcgat gatcttggtc agcaccccgg cggccggcga
     1081 cgggatttcg gtgtcgacct tgtcggtcga cacctcgacg agcggttcgt cgagttcgac
     1141 cgtgtcgcct tcctgtttga gccagcgcgt gaccgtaccc tcggtgacgc tctcgccgag
     1201 tgccggcatc tggacggaga aggccatgtc tttgactcct cgatcgctcg tggtcggcgc
     1261 agatcgacat ctggcttacc acaccgcggt gtggtcggtg tgcaactcaa gtcgatgtcg
     1321 aaaccatcct gtcattgcgc ctggatcggc acacctccag ggcgggccgc gctcgggtgc
     1381 ccgggtggtc caagtccgct tgctacggtg tggccaatag atcgcgggtg ggcggaggtt
     1441 cgatgccgga aattgagcag ttaccccaac atttcgtcga cagtgccgac ggtgtccgga
     1501 tcgcggtcta cgaggagggc aaccgcgagg gcccgaccgt cgtgctggtg cacggctttc
     1561 ccgacagcca cgtgctgtgg gacggcgtcg ttccgctgct ggcggagcgc ttccgcgtga
     1621 tccgctacga caaccgtggc gtgggccagt cgtcggtgcc caagccggtt tccgcgtaca
     1681 cgatggcccg gttcgccgac gacttcgccg cggtgatcgg cgagctgagc cccggccggc
     1741 cggtgcacgt cctggcccac gactggggat cggtcggcat ctgggagtac ctgagccggg
     1801 ccggcgccag tgaccgggtg tcgtcgttca cgtcggtgtc cggcccctgc caggaccaac
     1861 tggtcgatta catcttcggt ggtctgcggc ggccgtggcg cccgcgaacc ttcgcgcggg
     1921 cggccagcca ggcgctgcgg ttgacgtaca tggtgttctt ctcggtcccg gtgctcgcgc
     1981 cgctgctggt gcgcctggcc ctgtcggtca agccgatacg gcgcaacatc gtcggcaaca
     2041 tccccgacga gctgatccat cactccgcca agctggccgg cgatgccgcc cattcgatca
     2101 agacctaccc ggccaactat ttcagcgcgt tctcccggaa gcggccgatt cacgtcgtgg
     2161 acgtgccggt ccagctcatc gtcaacacca aggaccccta cgtgcggccc tacgggtacg
     2221 accacacgac gcgctgggtg ccgcggctgt ggcgccgcga catcaaggcc ggccactttt
     2281 cgccgatgtc gcacccgcgc gtgatggcgg ccgcggtgca cgacttcgcc gacctcgccg
     2341 aaggcaaaca ggtgagccgt gcgctgctgc gcgcgcagat cggtcgtccg cgcgaggact
     2401 tcggcgacac cctggtggcg gtcaccggcg ccggcagcgg aatcgggcgg gcgaccgcgt
     2461 tcgccttcgc gcgcgagggt gccgaggtgg ttgtcagcga catcgacgag gcggccgtca
     2521 agcagaccgc cgccgagata gccggccgcg gcggtgttgc gcacccgtat gtgctcgacg
     2581 tctccgacgc cgaggcggtc gagacgttcg ccgagcgggt cagcgcggcg cacggcgtcc
     2641 ccgatgtcgt cgtcaacaac gccggcatcg ggcacgccgg tggattcctg gacacgccgg
     2701 ccgagcagtt cgagcgcgtg ctggacgtca acctcggcgg tgtggtgaac ggttgccggt
     2761 cgttcgggcg acgcctggtt gaacgaggca ccggcgggca catcgtcaat gtggcgtcga
     2821 tggccgccta cgcaccgtcg caatcgctga gcgcctactg cacgtccaag gccgcggcct
     2881 atatgttctc cgactgcgtg cgtgccgaat tcgacgctgc gggtgtcggg ttgaccacga
     2941 tctgccccgg ggtgatcaac accaacatca tcaacaccac gcgcattgac gcgcccgcgg
     3001 gcaagaccga cgtggtcgtc gaccggcggg gccagctcgg caagctgttc gcgctgcgcc
     3061 actacgggcc cgagaacgtc gccaaggcga tcctgtcgtc ggtgaagaag aacaagccga
     3121 tccgcccggt ggcgccggaa gcctatgcgt tctacggcgt ttcccgcgtg gcgccccagg
     3181 cgctgcgcag caccgcgcga ctacgggtga tctaggcggc cgggctcaca ggcctgtcgc
     3241 cgccccctcg ttgcgccgca ccagcagcgc gccggcgact acgacgatcc cgacgaccgt
     3301 cagcggaatg gtccacgccc ggcggctccc gaccgacgac tggcactgcg cgacgtagtc
     3361 cgtgtgcggg atgagctggt taaggatcgg aatgtttgcc acgctcttgt cgttcgcgct
     3421 tttagccgcg gacaggtccg tggcgacggc gtttccgcag gcaatggaat tgccgttgcc
     3481 gtcggagatc gatacgggcg tcaacaggcc gattacgccg gccagcaaca gcgccgcacc
     3541 cattgcgatg atcaatcgtc gtaccgtcac gatctgacct cccgtcgact cgcaatcggt
     3601 gggaggttag ccgttgtacc tggcggctaa accaaaccgc gtgtggcgca ccgacacgag
     3661 cctgggttgc taaccgtttg ctaacccttt tcggcgatgt cggtcagcac cgcgaacatg
     3721 gtgcgcgtcg gcacaccggt ggcgcccttg ggcgtgtagc cccacgcgct gccggtgttg
     3781 taggcggggc cggcgacgtc gatgtgtgcc cagctcacgc cgtcggcgac gaactcgcgc
     3841 aggaataccc cggccaccag catgccggcg tagcgctgcc cgctgacgtt ggccaaatcg
     3901 gccaccgtgg acttcaagtc gtccttgagc tcgtcgggca gcggcatcgg ccagccgttc
     3961 tcgccgaccc gttgcgagat ctccgcgacc cggtcgcgga actcgtcgct gcccatcacc
     4021 ccgggtatcc gggcgcccag cgccacggtc tgcgcaccgg tcagcgtgga cgtctcgatg
     4081 aggtagtccg gatcgtcttc acacgcccgc acgatcgcgt cggccaggat gagccggccc
     4141 tcggcgtcgg tgttgagcac ctcgaccgtg gtgcccccgt actgggtgag cacgtcaccc
     4201 gggcgctgcg ccgtcgccga cggcatgttc tcggcgatcg gcaccgtggc gatcacgtcg
     4261 atggggagtt gcagctgcgc ggccagcgtg acggtcgcga tcaccgctgc ggccccggcc
     4321 atgtccgagg tcatgtagtg catcgaggcc gccggtttga tcgagatgcc gccggtgtcg
     4381 aaagtgacgc ctttgcccac cagcgccacc gtcttggccg tcttggcctg cttggggccg
     4441 gccagccgcg atccgcggtg gatcagccgc accagccgcg gggggcgcga cgaaccttgg
     4501 ccgacaccga tcactccgcc gtagccggcc ttctgcagcg ccttgtcgtc gagcacctcc
     4561 acctcgaggc cgacggattc acccaagacc tttgcgcgcc gggccaattc ggcggggaac
     4621 aggtggttcg gcggcgtgtt gaccaggtct cgggcagtgg cgaccgcggc cgcgacggcc
     4681 gcgccgtgcg cgctctgttt cttggcgtcc tttgacgtgg cgagcacggt gatcttgcgc
     4741 agccctttgt ctttcggcgc ggtcttgtcg ctacggaacg cggtgaaccg gtagccgccc
     4801 aggatcagcc cctccacggc gggcgaacag gtgtcgccac cggattcacc gggcagctcg
     4861 gtcagcgtcg tgatcagcgt ctcggcaccg cccagcgacc gcgccgccac cccggcggct
     4921 cgacggatcg tctcggcggg ccactcggag cgcggtttgc ccaggccgat ggtcagcacg
     4981 ctggccaccg gtagcgacgg caccaccagt cggtgtacct gttcgctgcc accggtggcg
     5041 cccagcgccc gaagaccgcc ctcgatttcg gcgaccgcct cggcaggcag gaacggctcg
     5101 gccgcggccc gaattacaga gacggcgccc ggcctgtctt cgtcgccggt cgacacgacc
     5161 ggcaccagca acaccgcatt agccgtgccg cgtttgggca gcgcggtggc gacgttgact
     5221 gacgggctct ggtaaccgga ttcgttgggc acgggcaact actctagccc gttcacccgg
     5281 ggccctgacc ggggccgacg tgcaggtcgt aggcggtcac gggggtgtca aaacccttga
     5341 gcgtcaacgg gccgagggcg gtcgcgggcc agtccggcaa cttgccgtgg agttcggccg
     5401 aggccaggat ctgcagcggt gccgcggtcg ccaccagtcg cgcggccagg ttgaccgggt
     5461 tgccgaagta gtcgccgttg atggccagga cggtgccgta ggcgagaccg gcacggacct
     5521 gcagtccctc gtcgcgtgcc cgcggatgat cgaccaggtc caccgcggcc tgcaccagct
     5581 gcccggccgt cgggctcacc cacatcacgg cgtcgccgat gaacttcacc acccggccgc
     5641 cgtcggcgtg gaccacatcc gcgacggtgg cggcgaactc gttgagcaac tcggacaact
     5701 gtgcgggtgt gagcgtctgg gtcagcgccg taaagctgga cagatccgcg aaaccgacgc
     5761 cgcacgtcac gctcgccgag gtgtcttgaa ggacaccctc gaaataggtt cgcgcgctga
     5821 tcaggtggtg gcggtgaacg gtgtccatca gcgctccgag ccgggggatg aattccgccg
     5881 ccgcgcgata ggcctgcgcg gtggccagct cgttgtgggt gtgggtcatc tggatgtcgg
     5941 gcatcccggc gcgaatcacg ctggcctcgg cctccgcgag tcgggccatg gcggcgccga
     6001 gcactcgcag catcccggac gcgccgtcgg cgccgactgc cgtcttgagc gcgagccatg
     6061 tcgccaggcc gtcgacatcg gcctggctca gcgcgggaat gtcgggacct gcgatggtca
     6121 gcccgagcaa ggcccacgcc tgcgcgacgt cctcggctga aaccccgagt tcatcggcgg
     6181 cggcctgcac ggtgtaaatg ggcggccccg accacagcac gacatcgccc gcaagcccga
     6241 acagccggcc gcgccgttcg gcctcgacga cttcgtcggc cgtgaagcca aggtcgtcta
     6301 gatacttgat cagatccgcg cgctcgcgcg cgttcgcgac tccggcagcc tcgagttcct
     6361 cgatatccag ggaatcgaag gaatcggcgg aatcgagggg gtcagacacc tacccaagtg
     6421 tgccagcagc acggcggatt ggtggcgatc gcaagcgcgg cggagccggg tgcggcgggt
     6481 cgtcgccaag gtgtctgggg tggcgatcgc aagcgcggcg gagccgggtg cggcggagcg
     6541 ccgccaaggt gtctagggtg ggacgccgtg accgagctac agcatggacc gctggaagac
     6601 cgccaccgcg agctgggcgc cagcttcgcc gaattcggcg gctggctgat gccggtgtcc
     6661 tacgccggca cggtcaccga acacaacgcc acgcgcaccg cggtcggcct gttcgacgtc
     6721 agccacctcg gcaaggcgtt ggtacgcggg ccgggcgccg cgcagttcgt caactccgcg
     6781 ctgactaacg acctcggccg tgtcggtccc ggcaaggctc aatacacact gtgctgcacc
     6841 gaatccggcg gcgtcatcga cgacctgatc gcctactacg tcgacgacga cgagatcttc
     6901 ctggtgccca acgccgccaa caccgccgcg gtcgtcaagg cgctgagcgc tgccgccccg
     6961 gccggcctga ccatcaccaa cgagcaccgc tcgtatgcgg tgctggccgt gcagggcccg
     7021 cgatccccag aggtgctggg cgagctgggc ctgccgaccg acatggacta catgggctat
     7081 gacgatgcct cgttttcggg cgtcccggtg cgcgtctgtc gcaccggata caccggcgag
     7141 cacggttatg aactgctgcc gccctgggac tccgcgggtg tggtcttcga cgcgctggcg
     7201 gcggctgtct ccgacgccgg cggcgagccc gccggcctgg gtgcccgcga caccctgcgc
     7261 accgagatgg gctatccgct gcacgggcac gagctttcgc tcgacatctc gccgttgcag
     7321 gcccgctgcg gctgggcggt cggctggaag aaggacgcgt tctacggccg cgacgcgctg
     7381 ctggccgaga aggcggcggg accacgacgg ttgttgcgcg ggctgcggat gatcggccgg
     7441 ggtgtgctgc ggccggggct gacggtgctg gccggggaca cccctgtcgg ggtgaccacc
     7501 tcgggaacgt tttcgccgac actgcaggtg ggcatcgggc tggcgttgat cgacaccgac
     7561 gcgggagtcg acgacggcca gcaaatcacc gtcgacgtcc ggggccgtcc cgtcgactgc
     7621 gaggtagtgc gcccaccctt cgtcgacgtc aaaacgcgtt agccgccagc ggttcacgtg
     7681 gcaaaaaccc gatggcaagc ggatatacaa tcggccccat gaccagcggc tcactcgggt
     7741 tcacggttgc gcgcgcggct caccccgcta gcgacgccga acgtgaatca attctggccg
     7801 agccgggatt cggcaaatac ttcaccgacc acatggcgtc gatcgactac gcagaaggcc
     7861 agggttggca caatgcgcgc gtcctcccgt acggcccgat cgagctggat ccgtcagcga
     7921 tagtgctgca ctacgcgcag gaagtcttcg aagggctcaa ggcatatcgc tgggtggacg
     7981 ggtcgatcgt gtcgtttcgc cccgacgcca acgccgccag gctgcgatcg tcggcccgtc
     8041 ggctcgcgat tcccgaactg cctgaagggc ttttcatcga atccctgcgt cagctcgtcg
     8101 ctgtggacag cgcctgggtg ccgcgagccg gtggcgaaga ggcgttgtac ttgcggccgt
     8161 tcatctttgc caccgagccg ggactcggcg tgcgaccgtc caagcggtat cggtacatgc
     8221 tgatcgcctc gccggcgggc gcgtatttca agggcggcat cagccccgtc accgtctggg
     8281 tgtcgacgga gtatgtgcgg gccagcccgg gaggcaccgg tgcggccaaa ttcggtggca
     8341 actatgccgc ctcgctgctg gctcaggccg aagccgccga aaacggttgc gaacaagtgg
     8401 tttggctgga cgccgtcgaa cgccgcttcg tcgaagagat gggcgggatg aacctcttct
     8461 tcgtgctcgg cagcggcggg accgcgcgac tggtcacccc ggagctgtcc ggctcactgc
     8521 tgccgggcat cacgcgggat tcgttgctgc agttggctct tgacgcggga ttttccgtcg
     8581 aggaacgcaa gatcgacatc gacgagtggc agaagaaggc cgccgccggg gagatcaccg
     8641 aggtattcgc ctgcggcacc gccgccgtca tcacccccgt ttcgcgggtg aagtacggcg
     8701 acaccgagtt caccatcgcc gacggccaac cgggtgaggt gacgatggcg ttgcgcgaca
     8761 cgctgaccgg gattcaacgg ggcaccttcg ccgatactca cggctggatg gcccggctgg
     8821 gctaatgccc ggccgagtgt gcggccagcc gcacattggg gcgcgaacgt gcggcccgcc
     8881 gcacgctcgg ccttaaagca ccctagaagc ggatcaatcc cgcgagcgcg accgcgctca
     8941 ccgtcgtggt cagttcgatc gcggcgccca gcacgtcacc gctgataccc ccgaacctgc
     9001 gcacgcagtg gcacaccagg gcggcgccgc agcacaccgc caccagcacc gccgtcggcc
     9061 cctgccacgg tcgcgacccc gccagcagcg aaacggcgag cagcacggcg atccaggccg
     9121 cggccaccgg cgcgggctgg ctgccggcga cgcgggcgcc caacgcgctg ccgtcggcgg
     9181 ccggcaccga ccggcgacag gccagcaccg ccgtgacccg gccggcgaag accgcgacag
     9241 cgattcccgc gggtcgcaaa gccgaaaagg ccaacccctg caacaggatc accaccacga
     9301 cggcggccac gccgaacggc ccggtcgacc cgtcgcgcat caccgccagc gcgcgttgtg
     9361 gtggcccgta gcagcccagc ccgtcggccg tatcggcgac accgtcgatg tgcaggcccc
     9421 gggtcgccag cagcaatgcc accacggcga gcagtccggg cagcgtgctg gacggcccga
     9481 aaacccagcc gccgcaccac gcgacggccg cggccaacgc tcccagcgcg gcgccgacca
     9541 caggcagcgc ggtcatggct ccccggccca tcggagcgtt tccggtgcgt gggatgggca
     9601 tcaccgtcgc gaacgcgaaa gccgttgcca aagaacgaat cacgacggcg tcgagctgga
     9661 cggaccgact acaccggcct cccggaaggt cgccatcgac aagagcgcgg ccaccgcggc
     9721 gcgcagcacc ggcagcgcca ccgcggcgcc ggtgccctcg cccagccgca tgcgcaggtc
     9781 caggatcggt tccagctcga gagccgccaa ggccaggccg tggcccggct cggtggaccg
     9841 gtggccggct tgccaccacc gccgggcccc gggcgccaga cgctccgcga ccaacgcggc
     9901 ggccgtcacc gccatgccgt cgagaagcag cggggtgcgc cgcaccgcgg cctgtgcgca
     9961 aaagcctgcc atcgcggcca gatccgcgcc gccgcaacag cgcagcagcc cgaccgcgtc
    10021 gggcaacacc cgccgcgacc gaaacagcgc atcgcgcacc gcggccgtct tgcgtgccca
    10081 tgccgcgtcg tcgatgccgg tcccgaagcc caccaccgcg accggctcgg tgttcgtcag
    10141 cgccgccacc aaaaccgccg ccggggtggt gtttccgatc cccatgtcac cggcgatcag
    10201 caggtcggct ccggcgtcga cctcctcgtc ggcgatggcg cgccccgcgg cgatcgcttc
    10261 aacggtctcc gcgtcggtca acgcgtcctg cacggtgatg tcgccgctgc cgcgccgcac
    10321 cttgtgggcg ccgatccgct ccgaggccgc gtcggcgtcg accgccaggt ccgctacccg
    10381 caccgtggcg ccggcgacat cggccaacac gttgatcgcc gcgccgccgg cgtcgatgtt
    10441 ggcgaccatc tgggcggtca cctccggcgg gaaggccgac accccggacc gtgcgacacc
    10501 atggtcaccg gcgaagacca ccacccgggg gcgctcgaat tgggttggcg gacagtgccc
    10561 ttggcaggac gcgacccaga ccgacaggtc ctcgagccgg cccagggcgc cccgcggctt
    10621 ggtcagggtg tcctgacgag cgcgggcggc tgaggcggcg gccgcatcgg gcagcgccac
    10681 cggcgcgaat tccatcaggc ccccggctgc ttgagcgaca gcgcctgccc ggccaccacc
    10741 agcaccaccc gatcgcacaa ctcggcgacg cgctggttga gcgaacccag ttcgtcggcg
    10801 aaccggcgcc cggaggcggt ggcgggtata acggtcaacc cgacttcggg actgacgagc
    10861 accagggtag aaccgaacgc gccgaccgcg ctgatcattt cgtcaacggg agcggccacc
    10921 gatcccttct cccaggcgtc gttgcggtcc agtgtgccgg tcagccagcc accgaggtcg
    10981 tcgacaagtg tcggggtgtc cggcgactgc cgcaattggg ttgtgatgtc gtcggtttcg
    11041 accgttgacc aatgcgcggg ccggcggctg cggtgctgcg cgacccggtc ctcccacgcc
    11101 gcatccgcct gagcggtcac cccggaagcc aggtaacgga ccggctggcc gggtggcagc
    11161 gacttggcga cggcctcctc cgcccaccgg gacttgcccg acctgatccc gccgagcacc
    11221 aaggtgcgca ccggattcag gctgccttgc gacgatggct aatcgacact gctgccgcgc
    11281 tcgacctgag gccgaggtgc cctcaaccgg cgcatttggg aagcccggga cgcggcgtac
    11341 aggcccagct tccaacggct ttcggtgttc ttcgggaact tcgcatcgac ccgcctgttc
    11401 accttgcggc cgaggatcag cccgtcgacg gacatcacgg ccatcagcac aagcatcgcc
    11461 ggcgagacat agagctgcag ctgggggacg gcgaacatga tgaacaacag gactaccgtc
    11521 gccggcatga acaggccgag gacgttgcgc cgggcgtcca ccacgtcgcg cacgtaacgc
    11581 cgcaccggcc cctgatcccg cggcagcagg tatgcctcct cgccggccat catgcggtcc
    11641 cggcgctcgc tcatccgggc ccggctcaca gtcttttcgg ctttgcgctc ctcgcggctg
    11701 agtttcgcgc cggcgagcga cttgcgccgg gcccgcgcct cggatgaggt catgggtgcc
    11761 ggtgcgaccg gacccttttt cgggttgcgc gtggcctggc tgcgcttgcg cgtcggccga
    11821 cccttggggc cggtcctgcc cgagccacga gagccggcgt ccagggaact ggcggcatcg
    11881 accacggtcg gcgtcacccg gccctccgcg ctgccctcag cgtcgtctcc atggcctttc
    11941 ttacggccga tcagcttcac agtggccagg ttacttcgcg gtcgctccgc accgtaatgc
    12001 ccccggaatg cgttgttatt agtcgtgtgc cagcgtgtcc cttacaccta cgcttgactg
    12061 tgatggatgg ctacctttga tgaacgacgg cacgatggcc tcggatgatg ctgccgctgg
    12121 cctcgcgccc ggtcgtctcc ggttgccggc catgcaggtt ttggtcgccc cggattgtta
    12181 tggcgacagc ctgtcggccg tggaggccgc cgcagccatc gcgaccggct ggacccggtc
    12241 acgcccgggc gatcggttcc ttgtcgcccc gcagtccgac ggcggccccg gtttcgtcga
    12301 ggtgctctcg aaccggctgg gacagaggcg ccggatgcgg gtgtgcgggc cgatggacac
    12361 ctcggtcgag gccgagtggg tgttcgaccc cgcctcggcg accgcgtacc tggaatgcgc
    12421 gcaggcctgc gggctggcgc tgctcgacgg tccgcccact ccggaaaccg cgctggcggc
    12481 acacagcagg ggagtggggc agctcatcat cgaggcgctg cgggccgggt cggtgcggat
    12541 cgtggtgggg ctgggtggca gcgcgtgcac cgacggcggg cagggaatga tcgccgagct
    12601 cggcggactg caagccgccc ggctacggct ggccgaggtg caggtgatcg ccgcctcgga
    12661 caccgaatac cccatggtgg gcccctgggg cgcggccagg gtgttcgggc cgcagaaggg
    12721 cgcggacacg gccgcggtcg cggcgctgga agcccgtctc gaggcgtggg cgatcgagct
    12781 ggaagcggtg gccggacggg acgtcagctc ggagccgggt gcggccgccg ccggtggcat
    12841 cggcgccggg ctgcttgcgc tcggcggccg gtgcgagtcc ggcgcggcca ttatcgccgc
    12901 gcacacccac ttggacgacg accttgagac ggcggagatg atcgtcaccg gcgaaggccg
    12961 gctcgacgag cagtccctgc acggcaaggt ggtggggttt ctcgcggatg cggcccgtcc
    13021 gctgggaatt ccggtgatcg tgttggccgg ccaggtcggc ctggacaact cggcggtgcg
    13081 ctcgtcgggc atcatgtccg ccttgtccat cgccgattac gccggttcgg tgcgcttggc
    13141 gcaggccgat gcggcaaacc agctaatggg attggcgtca caggtggccg cgcgactcgg
    13201 gaatagcggt cctgcaaggt accgttgatg cagtgggttt aaccgagcgg ttcggcgtgc
    13261 ggggcccatc caacaatcag gcatgtagga gaagcaatga cggtgcaaaa cgagtcaacc
    13321 gccaagaccc acggcgcgat cctgaccgaa gccgccgctg ccaaggcgaa gtccctgctt
    13381 gaccaggagg gtcgcgacga tcttgcgctg cgtatcgcgg tccagccggg cgggtgcgct
    13441 gggctgcgct acaacctctt tttcgacgac cggacgctgg atggcgatct gacggccgac
    13501 ttcggcgggg taacgctgac ggtggaccgg atgagcgcgc cctacatcga gggcgcgtcg
    13561 atcgacttcg tcgacaccat cgagaagcag ggcttcacca tcgacaaccc gaacgccacc
    13621 ggttcgtgcg cctgcgggga ctcgttcaac tgaccggcta gccgggctag ccgggctgcg
    13681 tcggttgccc gcccgcagcc gctagtacga gcttgcggtg cggagcacgt acgagcacac
    13741 catgatctgg ccccgctgga aaagcagctg tgcgatcccc tgcacatccc cacgcgcagg
    13801 gccgccgatc gcgggcgtta cccgcatggt gaacagcgcc tgggcttgat actgggatag
    13861 gtacacgatc ttgtcgaccg cggtcagctc gacctcggag aactgcttac ggaaagcgtc
    13921 gctgctcatt ttggcgagcg cctgatcggc gcgtttgtca cgcacgccgt catacatgcc
    13981 gcataacgcg tttcggacga tggcgtcgac gtcgcggcgc tccagcgcgt ccagataacc
    14041 ctggatcgcg cttttggccg ctccctcgga gaacgaggag ccggtattgg ccccgttggt
    14101 gcgcaccccg tacacgatcg cgaccgtcat cgcggccacc agcgcgacgg tgaccagcat
    14161 cccgatgatc aggcgccgct tggaacgtcg ccgcgggtag atcatgggcg gctgcccggc
    14221 ataattggcc ggcgacagtc ccgcgtcgct gggtggcggg gggcggaagg cgccggcggt
    14281 gggttcatcg gggaactcaa gcggctgggc ctcggtgtgg ggcgttggtc cgtcggtgcc
    14341 gacagtgtgg ttcggcgggt gcggaccggc catcgtggct ctcctacggt ggtagacggg
    14401 accatgggcg gggtggccgc gtctgggggt cccggcgact tgagcgagtt gcttgagtcg
    14461 tttctaggca ggctagcgca cgactttgcg acgaccgtga cgcgacgacg ctggatgggc
    14521 ttgagtaagc tagataacct tactaacgaa ggatggagat ttcgtgacga tcgcggtaac
    14581 aggttcgatt gcgacggaca atctgatgcg gttccccggc cgattctccg agcacctgct
    14641 ggccgaccat ctgcagaagg tttcgctgag cttcctggtc gacgacctgg tcattcaccg
    14701 cgggggagtg gcgggaaaca tcgcgtatgc catcggggtg ctgggtggcg acgtcgcgct
    14761 cgtcggggcc gccggcgacg acttcggcga ctaccgcgga tggctgcaaa gccacggtgt
    14821 caattgcgat cacgtgctga tctccgagag cgcgcacacc gcccgtttcg tctgcaccac
    14881 cgacctcgac atggcccaga tcgcgtcgtt ctacccgggc gccatgtccg aggcccgcaa
    14941 catcaagctc gccgacgtcg cctcgtccgt tggcgcgctg gacctggtca tcatcggtgc
    15001 caacgacccg gaggcgatgg tggtgcacac cgaggagtgc cgcgagctgg gtctggcctt
    15061 cgcggctgac ccgtcccagc agctgccccg gttgtccggt gatgagatca gcaagctgat
    15121 cgacggcgcc gcgtacttgt tcaccaacga ctacgaatgg gaactgttgc tctccaagac
    15181 cggctggtcg gagtccgacg tgatggagaa ggtcgacctg cgggtgacga cgctgggcgc
    15241 caagggcgtc gacatcgtgg agcgcgacgg caccaccgtc cacgtcggcg tggtgcccga
    15301 gaccagccag accgacccca ccggcgtcgg cgacgcgttc cgggcgggtt tcctgaccgg
    15361 ccgcagcggt ggtctcagcc tcgagcgttc ggcgcaactg ggctcgctgg tcgccgtgct
    15421 ggtgctggaa tccacgggaa cccaggagtg ggactggaac cacgaggtcg cggtcaatcg
    15481 gttggccggc gcttacggcg aggacgccgc cgccgagatc gccggcgtgc tggcctgaag
    15541 ccgtcgagcg tgagctcagg gcgtcgaggg tgtagccacg gcgggatttc ggcgaaattc
    15601 gctgccacgc ttcacgctcg gcgcccaggc ttcacgctcg aagtggtcga ggagggcggg
    15661 gtgcggcccg gcgctagagc tgcaccgggt atacgggctc gctgatctgc ggcaccacac
    15721 tgtgctcgac gaagatcgcg tgccagagca tgaagatgag cagcgtccag aggcggcggc
    15781 tgtgatcgct gatgccgctt cggtgctcgt cgagcatcct gcgcacggcg gccaggtcga
    15841 tcaggtgatc agcctgcgac gaatccacca acgcgtaggc ccactccagc agcgggcccg
    15901 cgcgtagcca gtgccggatc ggcaccggga atccgagctt gggccggtgc aacacgtgtg
    15961 ccggaacgat cggttccagc gcgcgccgta gcgcgtactt ggtggtggtg cgggtgatct
    16021 tcgcctctac cggcagccgg gaggcgaccg cgaacacttc gggatccagg aacggcaccc
    16081 gaagctcgag cgagttcgcc atcgtcatct tgtcggcctt gaccaagatg tcgccgcgca
    16141 accaggtgaa caggtcgacg tgctgcatgc gggcgacggg gtcccagccg gccgactcgg
    16201 cgtacaccga ggcggtcaca tcggtgtggg tccagtcgtc gcggaacccg ggcagcacgt
    16261 cgcgcagctg cacgtccgag aagctgcggg cgttgccgta gtagcgctcc tcgagcgtca
    16321 acgatccacg gtgcagcagg ctcttgccgc gcatgccgtc cggcaacggc ttggacacct
    16381 tgcccatgga acgccgcagc ggactgggca gatagtcgaa cggcttcaac gacagcggct
    16441 cgcggtagat cgtgtagccg ccgaacagct cgtcggcgcc ctcaccggac agcaccacct
    16501 tgacgtgctt gcgggcctcc cgggcgacga agaacagcgg caccagcgcg gggtcggcaa
    16561 ccggctcatc gaggtaccag acgatctcgg gcagggcggc gacgaactca tcggggctga
    16621 cgaccttggc gatatgccgg gcgccgatgg cctcggccga cgccaccgcg acgtcgatct
    16681 cggaaaagcc ctcccgctcg aaacccgtcg tgaacgtgat cagccggggg ttgtgccgga
    16741 tggccagcgc cgcgatcgcg gtggagtcga tgcctccgga cagaaacgcc ccgacggtga
    16801 cgtcggcgcg catgtgcttg gccacggagt cctccaacac cgcggtgatc tcgtcgtagc
    16861 gggcctgttc ggtgtcgcgg ttgaacggta tggcgttgaa gcgcggcacg aagtagcggg
    16921 tgacttccgg ctgctggcct gggcggatcc gcgcgtagca gcccgactcg agtcggcgta
    16981 ccccgcggtg cagcgtttcg ggctccggca cgtactgcag gacggtgtag tgctgcaccg
    17041 ccctgtcatc gatgccggtg tcgaacccga ccaactcggc cagatccatc aggcatttct
    17101 tctcgctggc cagcgcggtg ccgccggtgc cggtggccat gaacagcggt ttgatgccga
    17161 acgggtcccg ggcacagaac aattcacggg tctcggtgtc ccacagcgcg aacgcgaaca
    17221 tgccgcgcag tcgcgtcaac acgtcggtgc cccagtagtg gtagccggcg acgatggcct
    17281 caccatcacc gtcggtggcg aaaacggcgc cgtgccgggt ggccagctcg tcgcgcagct
    17341 cgaggtagtt gtagatctcg ccgttgaaca ccagcacgta gcggccgggc gcctccggcg
    17401 gcccccaccg cagcggctga tgcgaatgcg cgatgtcgat gaaggacaac cggttgaacc
    17461 cgaacacgac ggccccatcg gcgtcggggg cagccaatcc gccgggctcg tcgggaccgc
    17521 ggtggcgcat caggcgcaac gcgtgggaaa tggtgccgtc ggcagccgcg gcgtcgatgc
    17581 cacggctgcc ggcaccggcc ggggccgcga cgaacgcgag cagaccacac acggcgcccc
    17641 agtatgccgc actcgggtga cgcgtgcgga cctgcacgtc cggcgggtcg tggtgcgcgt
    17701 gccgactgac cggccggggt ttcgtcgccg gggtgggccg cgtggtctac gctgcgtagt
    17761 attcgatatc cgagcaggag gcgccaacgt gacacctcgc gagcaggtcc gttcgcaacg
    17821 tttgtcgcat ggcaggtttc agcgccgttc tggcagtgct aaccgccgtt tgccccgtcg
    17881 cctgcggacg ctggcgctgg tcgcgacatt cggcctgctg gcgctgaccc taagcggatg
    17941 tagctggtcg gaagcgctgg gtttgggctg gccggagggc atcacccacg aggctcacct
    18001 caaccgcgag ctgtgggtcg gggcagtgat cgcttccctg gtcgtcgggg tgctggtgtg
    18061 gggcctcatg ttctggtccg ccatattcca ccggaagaag gcgactgaca ccgaattgcc
    18121 gcgacagttc ggctacaaca tgcccctgga gctcgtgctc actgtgacgc cgttcctcat
    18181 catctcggtg ctgttctact tcaccgtggt ggtccaggaa aagatgctgc atctggccaa
    18241 ggatcccgag gtcgttatcg acatcacgtc cttccaatgg aactggaaat tcggttatca
    18301 gcgcatcaat ttcaaggacg ggacgctcac ttacgacggc gccgatcccg cgcgcaaaaa
    18361 cgcgatggta tccaagccgg aaggcaagga cgcccacggc gaggagctgg tcgggccggt
    18421 acgcggattc aatagccagg accggactta tctgaacttc gacaaggtcg agaccctggg
    18481 aaccagttcc gagattccgg tgctggtgct gccgaccggc aagcgcatcg aattccaaat
    18541 ggcgtccgct gacgtcattc ataatttttg ggtgccggaa ttcctgttca aacgcgacgt
    18601 gatgcccaac ccggtggcca acaactccgt caacgtgttc caggtcgagg agatcactaa
    18661 ggccggggcg tttgtcggtc actgtgccga gttttgcggc acctatcact cgatgatgaa
    18721 cttcgaggtt cgggtggtgc cgcccaatga cttcaaggcc tatttgcaac agcggatcgc
    18781 cggtaagacg aatgccgagg cgttgcaggc gatcgcccag tcgccgatag cggtgaccac
    18841 tcacccgttc gagacccgcc gcggtgaatt gaccacaagc cagtaggtga ggacattcga
    18901 tgcatattga agcgaggctt ttcgaattct gcgccgtttt ctttattttg gcggcggtgc
    18961 tctacggggt gctgacggca ctgttcgcca ccggcggcga ggagtgggcg ggcaccaccg
    19021 cgttgacgtt gaccggtggc ctggcgttga tcgtggccac cttcttccgg ttcgtggcgc
    19081 gccggctgga tactcggccc gaggactacg agggcgctga gatcagcgac ggcgcagggg
    19141 aattggggtt cttcagcccg cacagctggt ggccgattct ggtcgcgttg tcgggctcgg
    19201 tggcggcgac cgctattgcg ttgtggctgc cgtggttgat cgttgcgggg gtcgcgttaa
    19261 ttcttgcctc ggtggccgga ttggtcttcg agtactacat cggtcccgag aagcactgat
    19321 ttgcgcctca aaggtcacaa tcagggcatc agttgatcat tggcgcgttt gccaagactt
    19381 ctatgcccta gtccgttcgg tagggtttgc ccaggcagaa tgagaatctc gcgagcgcgc
    19441 gcgcaacgag gtggacgcgg agccccgcgc gctggtgatg cagttggaca gggcaggcag
    19501 acatgagcgg gccgaatcct ccgggatggg aacctgaaga acccgattcc gccggcgagg
    19561 gcggccatga accggagtcc gacggctccc gcgacgaatt ggcaccctcg gaactcgatt
    19621 ctcacgacga cctagcacac ctcgacgaca gcgccgcaat agcagcgcca gaccggacgg
    19681 gccagacgga tgcgtactca cgggcttact cggctccgga gtccgagcat ttcatcagcg
    19741 gttcctattt gccaggggat ctcaggctct atgaatacga cggctatgag gagtcatccg
    19801 agtccgatga cgaagagggt gccccccggt ggccgtgggt ggtcggcgtc gccgccatcc
    19861 tggccgcgat cgcgctcgtg gtttcggtgt cgctgctcgt ggcgcgcacc gacaccagca
    19921 agcttgccaa ccccgggacc accaccaccc cgtcgcagcc gccaatgcaa gacgaaataa
    19981 cgacgactaa gcccccgcca cccccaccac ccccgccgcc cccgcccagt accgagaccc
    20041 ccactgctac ggagacccag acagttacgg tgacacctcc tcctccaccc ccgccgcccc
    20101 cgccggcggc cccggcgcca cctccggcga cgtcgacgcc ggccgccgcc ccgccgccat
    20161 cgaccacggc ccctgccggt ccgcgccagg tcacgtactc ggtgacgggc accaaagccc
    20221 cgggcgacat catctcggtg acctacgtcg acgcctctgg tcgccgccgc acccagcaca
    20281 acgtctacat cccgtggtcg atgaccgtca ccccgatctc gcaatcagac gtcggctcgg
    20341 tggaggcgtc cagcctcttc cgcgtcagca gactcaattg ctcgatcacg acgagcgacg
    20401 gaaccgtgct gtcctccaac accaacgact taccgcagac gagctgctga tggtcagcaa
    20461 atattctgcc tatcggcgtg gatcggaaac cgttccgccg gacgtggtcg accgcatatt
    20521 ggttgggacg tgcgccgcca tttggctggt gttgttgggc gtgagcgtgg ccgccgccgt
    20581 cgcactggtg gatctgggtc gcggctttca caagatggca ggcaactcgc acaccacgtg
    20641 gttgtacgcc gtcatcattg tttccgcgtt gatcatcgcc ggagcgatcc cggtgctgtt
    20701 gcgggctcgt cgcatggccc ccgccgaccc ggctgcgcgg tcggtgttgg gttcgaccag
    20761 gccgctggtt cgcctcatgt cgcccgcgcc ccgcactgcg gcggagcggg cgcgtcagca
    20821 gcgggtggtg cgggcggagg agccggcggg cgagtggtcg ggcgtggacg tggatcgggt
    20881 ctggttgcgc ggcacggtcg ttctgacggg cacgatgggc gccgccctga tcgcggtagc
    20941 gacggcaacc tatctgatgg ccgtcggtca cgagggcgcg tcctgggttg tctatgggct
    21001 ggccggtgcc atcaccgcgg cgatgcctgc ggtcgagtgg ctgcatattc gccagctacg
    21061 cagtgtggtc tccgcgcgcg tggccgctta gccgtaccgc gcgcgggat
//
LOCUS       DASSGQ010000196         6220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_14206, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000196 DASSGQ010000000
VERSION     DASSGQ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14206"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VFI55_08625"
     CDS             complement(<1..1194)
                     /locus_tag="VFI55_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003914532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7074127.1"
                     /translation="MAHKPPTAVIESAHRGHALPLDDTTDFDNADRGFIAALSPCVIK
                     AADGRVVWDNDAYSFLGGPAPTSVHPSLWRQSTLAAKQGLYEVVPGIYQVRGLDISNI
                     TFVEGDTGVIVIDPLVSTEVAAAALALYRAHRGGHRSVVAVIYTHSHADHFGGVLGVT
                     SQADVDAGAVAVLAPEGFVEHAVQENVYAGPAMTRRATYMYGTLLARGPLDQVGCGLG
                     QATSTGEVAVIVPTIDIRVTGEKHTLDGVEIEFQMAPGTEAPAEMHFYFPRFRALCMA
                     ENATHNLHNLLTLRGALVRDPHAWSGYLTEAIDSFSDRADVVFASHHWPTWGRDKIVE
                     FLSLQRDMYAYLHDQTLRLLNRGYTGVEIAEMFQLPPALERAWHTRGYYGSVSHNVKA
                     VYQRYM"
     gene            1368..1847
                     /locus_tag="VFI55_08630"
     CDS             1368..1847
                     /locus_tag="VFI55_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LpqH"
                     /protein_id="HET7074128.1"
                     /translation="MKRGLTVAVAGAAILAAGISGCSSNKSSTGGGGTSSSGASASAS
                     AGGGPKVSIDGKDQNVAGSVVCTTAGGTVNIAIGGAATGIAAVLTDANPPQVKSVGLG
                     NVNGVTLGYTQGTGQGNASATKDGSSYKITGTATGVDMANPTQPVNKPFEIDVTCSS"
     gene            complement(1831..3360)
                     /locus_tag="VFI55_08635"
     CDS             complement(1831..3360)
                     /locus_tag="VFI55_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7074129.1"
                     /translation="MYSSRPARKRRVWSLRLRLLVGQIVVLALVCIGITAVTELALNH
                     HLVRQLDGQLAGTSYRSALMYPEPNRPGWRHEPHFYYPRPGPGPRFLDAPGQPAGMVA
                     AVVSGGKTVDAGYTTSSGARAALTDKAQAQLASISDTRNPVTVNLDGLGRYRVVAAPS
                     RRGGDVIVTGLSMSDVDATLVQMLVIFAIVTVVALAAATIAGVVIVRRALAPLRRVAQ
                     TASMVADLPLARGEVEMPVRVAESDANPSTEVGQLGSALNRMLDHIVDALSARQASET
                     RVRQFVADASHELRTPLAAIRGYTELTQRMGDDREAVAHAMSRVASETERITRLVEDL
                     LLLARLDSGRPLESEPVDLSRLAVDAVSDAHVAGPEQQWELDLPEEPVIVTGDAARLH
                     QVLTNLLANARVHTGAGTVVTTRLSAEPSHTVLRVIDNGPGIPAALQSEVFERFARGD
                     SSRSRKGGSTGLGLAIVSAVVKAHHGTITVNSSPGHTEFTVRLPLNGWQPPASAPSSN
                     R"
     gene            complement(3338..4042)
                     /locus_tag="VFI55_08640"
     CDS             complement(3338..4042)
                     /locus_tag="VFI55_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7074130.1"
                     /translation="MCRADGNPINVLVVDDEAVLAEMVSMALRYEGWNISTAGDGSSA
                     IALARAQRPDVVVLDVMLPDMSGLDVLHKLREENPQLPVLLLTAKDSVEDRIAGLTAG
                     GDDYVTKPFSIEEVVLRLRALLRRTGVTTVDSGAQLVVGDLVLDEDSHEVTRAGEPIS
                     LTSTEFELLRFMMRNSKRVLSKAQILDRVWSYDFGGRSNIVELYISYLRKKIDNGRDP
                     MIHTLRGAGYVLKPAR"
     gene            complement(4193..4348)
                     /locus_tag="VFI55_08645"
     CDS             complement(4193..4348)
                     /locus_tag="VFI55_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6131 family protein"
                     /protein_id="HET7074131.1"
                     /translation="MVVLGIVLLILGFVFGIHLLWVLGIVLLVIGAVLWIMGSVGRPV
                     AGRRYWY"
     gene            complement(4360..4596)
                     /locus_tag="VFI55_08650"
     CDS             complement(4360..4596)
                     /locus_tag="VFI55_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074132.1"
                     /translation="MRGSGIIGAIVLVWLLIGVFAAYQRDYFKGGSTSCVTAGNIALT
                     VVAGPLNYAGVNPKVKDCHVPQPSSMSSGLVALN"
     gene            complement(4727..5137)
                     /locus_tag="VFI55_08655"
     CDS             complement(4727..5137)
                     /locus_tag="VFI55_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET7074133.1"
                     /translation="MAQRRARVSDVHRIAASMPHVKRLEGPKGNSIYQVGGKSFVFFR
                     TAQPDATDPDTGERYADVIMLWVESEGDKLALIQDPDSPFFTTNHFDGHSSVLLRASR
                     LAEITTTELAELIQDAWLSRASKKRAAAWLAEHP"
     gene            5193..6068
                     /locus_tag="VFI55_08660"
     CDS             5193..6068
                     /locus_tag="VFI55_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019927528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HET7074134.1"
                     /translation="MAAARCRAIARVLHPRVRSQADGSHYTSPGELPLVPGIDGVGRT
                     PDGTLRYFVLPDSAIGAMAEQTVIDRRHSIVLPDSADAVLVAAAMNPAMSSWIALRQR
                     TRFEPGQTVLVLGATGSSGRMAVQVAKRLGAGQVIGAGRDARRLSVLGSLGADATFQL
                     DNAAAVTDLAQAARDVDVIIDYLWGQILTDVMAGIAGNRSDGGKPLTWIEVGSVAGAS
                     APIPSAALRAVRLQIVGSGQGPVPTRDILTELPALAGEITSGTFHIDARGVPLADVES
                     AWNDTDAERRIVITP"
BASE COUNT         1104 a         2045 c         2013 g         1058 t
ORIGIN      
        1 catgtagcgc tgatagacgg ccttgacgtt gtggctgacc gatccgtagt agccgcgggt
       61 atgccaggcc cgctccagtg ccggcggcag ctggaacatt tcggcgatct caacacccgt
      121 gtagccccgg ttgagcagcc gcagcgtctg gtcgtgcagg tatgcgtaca tatcccgttg
      181 cagcgaaagg aattcgacga tcttgtcccg tccccacgtc ggccaatggt gcgatgcgaa
      241 caccacgtcg gcccggtcgg agaagctgtc gatggcctcg gtgagataac ccgaccaggc
      301 atgcgggtcg cgcaccagcg cgccacgcag ggtgagcagg ttgtgcaggt tatgggtggc
      361 gttctcggcc atgcacaacg cgcgaaagcg tgggaaatag aaatgcattt cggcgggcgc
      421 ctcggtgccg ggcgccatct ggaactcgat ctccacgccg tcgagggtgt gcttctcacc
      481 ggtcacccgg atgtcgatgg tcgggacgat cacggctacc tcgccggtgg aggtagcttg
      541 gccaagcccg cagcccacct gatccagcgg accgcgtgcc agcagggtgc cgtacatgta
      601 ggtcgcccta cgtgtcatgg ccggaccggc gtagacgttc tcttggacgg catgctcgac
      661 aaagccttcc ggtgccagca ccgcgaccgc accggcgtcg acgtccgcct gcgaagtcac
      721 gcccagtacg ccgccgaagt gatcggcatg actgtgggta tagatcaccg cgaccacgga
      781 acggtgaccg ccgcggtggg cgcgatacag ggccagggcc gcggcggcta cctcggtgga
      841 aaccaaggga tcgatgacga tgacgccggt gtcgccctcg acgaaggtga tgttggagat
      901 gtcaagcccg cgaacctgat agatccccgg caccacttcg tagagacctt gtttggcggc
      961 cagggtggat tgccgccaca ggctcgggtg caccgacgtc ggtgccgggc cgccgaggaa
     1021 tgagtaggcg tcgttgtccc acaccacgcg gccgtcggcc gccttgatga cgcatgggga
     1081 cagcgccgcg atgaacccac ggtctgcgtt gtcgaagtct gttgtgtcat caagcggcag
     1141 ggcatgtccg cgatgggccg actcgatgac ggcggtagga ggtttgtgag ccacggcaac
     1201 acattgccac tagtgcgccg ctcgccggaa gggacgcgtt gaggtccgcc gggttaaccg
     1261 aagcgaaaca atttcgccca acctcttctt tgcgacccgg caaaccagca tactgccttg
     1321 acggctctca atgccgagga ccgcaacgat cgggtaaagg agaacaggtg aagcgtggac
     1381 tgactgtcgc ggtggccgga gcggcgattc tggctgcagg catttccggg tgttcgagca
     1441 acaagtcgag tacgggcggc ggtggtacta gttcctcggg cgccagtgcc tcggcgagcg
     1501 ccggcggagg gccgaaggtc agcatcgacg gcaaggacca gaacgtagcc ggctcggtcg
     1561 tgtgcacgac ggcgggcggc accgtgaaca tcgcgatcgg tggggccgcg actggcatcg
     1621 ccgcagtgct caccgatgcc aaccctccgc aggtgaagtc ggttgggctc ggcaacgtca
     1681 acggcgtgac actcgggtac acgcagggga ccgggcaggg caacgcttcg gcgaccaaag
     1741 acggcagcag ctacaagatc accggcaccg cgacgggcgt ggacatggcc aacccgacgc
     1801 agccggtgaa caagccgttc gaaatcgacg tcacctgttc gagctaggtg ctgatgcggg
     1861 cggttgccac ccgttgagtg gcaaccgcac cgtgaactcg gtatgaccgg gagagctgtt
     1921 caccgtgatc gtcccgtggt gcgccttgac gaccgcggag acgatcgcta gtcccagccc
     1981 ggtactaccg cctttgcggg agcgcgacga atcgccgcgg gcgaaccgct cgaaaacctc
     2041 ggattgaagc gctgccggga tgccgggacc gttgtcgatg acccgcagca ccgtgtgcga
     2101 cggttcggcg ctgagtcgcg tcgtcacgac ggtgccggcg ccggtgtgga ctcgcgcgtt
     2161 ggcaagcagg ttggtcagca cttggtgcag ccgcgccgca tcgccggtga cgatcacggg
     2221 ttcttcgggt aggtcgagct cccactgctg ctccggcccg gcaacgtgtg cgtcactgac
     2281 cgcatcgacc gccaaccggg acaaatcgac gggttcgctt tccagcgggc ggccggagtc
     2341 caggcgcgcc aacagcagca ggtcctcgac gagccgggtt atccgctcgg tctcggatgc
     2401 cacccggctc atcgcgtgcg ccaccgcctc gcggtcgtcg cccatccgtt gggtgagctc
     2461 ggtgtagccg cggatcgcgg cgagcggcgt acgcagttcg tggctggcgt cggcgacaaa
     2521 ctggcgtacc cgggtctcac tggcctgccg tgcggacagt gcgtccacga tgtggtcgag
     2581 cattcggttg agcgccgaac cgagttgacc cacttcggtg gacgggttcg cgtcggactc
     2641 ggccacccgc accggcatct cgacctcgcc gcgggccaac ggcaggtccg cgaccatgct
     2701 cgcggtttgc gcgacgcggc gcagcggcgc cagcgcacgc ctgacgatca caacgccggc
     2761 gatcgtcgcg gcagccaggg cgaccacggt gacgatcgcg aaaatgacca gcatctgcac
     2821 cagcgtggcg tcgacatcgg acatcgacag accggtgacg atcacgtcgc cgccgcgtcg
     2881 gctcggcgcg gcgaccacgc ggtagcgtcc aagcccgtcg agattcacgg tgaccgggtt
     2941 gcgggtgtcc gagatcgacg ccaactgggc ttgggccttg tcggtcaacg cggcccgcgc
     3001 accgctgctg gtcgtatagc cggcgtcaac cgttttgccg ccgctgacca ccgctgccac
     3061 catgcccgcc ggctggcccg gggcgtcgag gaaccgtgga cccgggccag gcctcgggta
     3121 gtagaagtgc ggctcgtgcc gccatcccgg acggttcggc tcggggtaca tcagcgccga
     3181 gcggtaggag gtcccggcga gttgtccgtc gagctgtctc accaggtggt ggttcagcgc
     3241 tagttcggtc accgcggtga tcccgataca caccaatgcg agaacgacga tctgccccac
     3301 cagcagccgt agccgaagcg accagacccg ccgtttccta gcgggccggc ttgagtacat
     3361 agcccgcgcc acgcagcgta tggatcatgg ggtcgcgacc gttgtcaatc ttcttgcgca
     3421 ggtaggaaat gtacagttcg acgatgttgg accggccgcc gaagtcgtag ctccacaccc
     3481 ggtcgagaat ctgcgcctta ctcagcaccc gcttggagtt gcgcatcatg aaccgcagca
     3541 gctcgaattc ggtggaggtc aacgagattg gttcgccggc acgcgtcacc tcgtggctgt
     3601 cctcatcgag caccaagtcg ccgaccacca gctgagctcc gctgtccacc gtcgtcaccc
     3661 cggtgcgccg cagcagggcc cgcagccgca gcaccacctc ttcgatgctg aacggtttgg
     3721 tgacatagtc gtcgccaccg gcggtcaacc cggcgatgcg gtcttccacc gaatccttcg
     3781 cggtcagcag cagcaccggc agctgcggat tctcttcgcg cagtttgtgc agcacgtcga
     3841 gcccgctcat gtcgggcagc atcacgtcga gaacgaccac gtcgggccgt tgggcacggg
     3901 ccaaagcgat ggccgacgat ccatcgccgg ccgtcgagat gttccagccc tcgtaccgca
     3961 atgccattga caccatttcg gccaggacag cctcgtcgtc cacgacgagc acattgatcg
     4021 ggttaccgtc ggcacggcac atgacaaccc gttctacgga ggctcgggac tgcgtcacag
     4081 attccagtat ccgcacctgc ctgagccgac gctatgccga tcctttgcgt gtcctgtgaa
     4141 agatcgcatg actgcgatgc agtcacgcga tctttcattc ggaactcgat gcttaatacc
     4201 agtaccgccg gccggcgacc gggcggccca ccgaacccat aatccacagc accgcgccga
     4261 taacgagtaa gacaatgccc agcacccaca acaaatgaat gccgaagaca aatccgagga
     4321 taagcaagac gatgcctaga acgaccatga cgacttcctt tagttgaggg ctactaggcc
     4381 actggacatt gaactgggct gcggtacatg gcaatccttg actttcggat tgacacccgc
     4441 gtaattcaac ggccctgcga ccacggtcag cgcgatgttt cccgctgtga cacagcttgt
     4501 tgacccaccc ttgaagtaat cgcgctggta ggcggcgaag actccgatga gtagccatac
     4561 caaaacgatc gcgccgataa ttccgctgcc acgcatggtg acctccgttg tgttcgagaa
     4621 agactttctc gtgcggctag cgttgcccgt tcaactcacg tctaaacatt tgccgtgggt
     4681 tggtttcacg cggtgtggca caccgccccg acgagcccgg cccgctttag ggatgctcgg
     4741 cgagccacgc cgcggcgcgc ttcttcgacg ctcgcgacag ccacgcgtcc tgaatcaact
     4801 cggcgagctc ggtcgtcgtg atctctgcca gccggctcgc ccgcagcagc accgacgaat
     4861 gcccgtcgaa atggtttgtg gtgaagaacg gcgaatccgg gtcctggatg agcgccagct
     4921 tgtcgccttc cgactcgacc cagagcatga tcacgtcggc atagcgctcg ccggtgtccg
     4981 gatcggtcgc gtccggctgg gcggtgcgga agaacacgaa cgactttccg cccacttgat
     5041 agatggagtt gcccttcggg ccctccaacc gcttgacatg cggcatcgac gcggcgatcc
     5101 gatgcacatc gctcacccgg gcacgccgtt gcgccatagc ggcgacacta ccgctcccgc
     5161 gagcgacgtt gcggtgaagg gggattgaca tcatggcggc tgcacgatgt cgcgcgatcg
     5221 cccgggtcct gcatccacga gtgcgctccc aggctgacgg ctcgcactac accagcccgg
     5281 gtgagctacc ccttgtgccg ggcatcgacg gtgtgggacg cactcccgac ggcaccctgc
     5341 gctacttcgt cctgcccgac agcgccatag gggcgatggc cgagcagacg gtcatcgacc
     5401 gacgccacag catcgtgctg ccggactccg ccgatgccgt cctggtggcc gccgcgatga
     5461 atccggccat gtcgtcgtgg atcgcccttc ggcagcggac ccggttcgag cccgggcaga
     5521 cagtcctggt cctcggtgcc accggcagtt cgggccgcat ggcggtgcaa gtcgccaagc
     5581 gcctgggagc gggccaggtg atcggcgccg gacgggatgc tcggcgactc tcggtcctgg
     5641 gtagtctcgg ggccgacgcc acctttcagc tcgacaatgc cgctgcggta accgatctcg
     5701 cgcaggcagc tcgtgacgtc gacgtaatca tcgactacct gtggggccag attttgaccg
     5761 acgtgatggc cggcattgcc ggcaaccgtt ccgacggcgg caagccgctg acctggatcg
     5821 aggtcggctc cgtcgccggc gcctcggctc cgatcccctc ggcggcgctg cgcgccgtac
     5881 gcctgcagat cgtcggaagc ggtcagggac cggtacccac ccgcgacatc ctgaccgaat
     5941 tgcccgccct ggccggcgaa atcacttccg gcacttttca catcgatgcc cgcggcgtgc
     6001 cgctggctga cgtcgaatcc gcatggaacg acaccgacgc ggaacggcgg atcgtcatca
     6061 cgccttagcg ccgcaccggc ccggtcggaa agactgggta gagccgctgc tgcgacctac
     6121 cagccgtgcc agtgcccgtg atcacgaggc tcgccgtcgt accagtggtc gtcgtggcag
     6181 cgaccgaaat cccagttgtg gccccagccc ggatcccacc
//
LOCUS       DASSGQ010000197        27015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_318007,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000197 DASSGQ010000000
VERSION     DASSGQ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 27015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27015
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318007"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VFI55_08665"
     CDS             <1..680
                     /locus_tag="VFI55_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET7074135.1"
                     /translation="NGSTVGVGQPIILNFPGTVEDAGAAINAVHVSSVPPVPGKFYWM
                     TPTQLRWRPLSFWPAHTAVTVDAGGTVSSFQTGDSLIATADDSTHQLTITRNGTVEKT
                     FPMSMGMSAGNHQTPNGTYYVQEKMPSVVMDSSTYGVPVNSTYGYKVTVELAVRFDNV
                     GDFVHSAPWSVDDQGKRDVSHGCINISPTNAKWFFDNFGPGDPIIVKNSTGGDYKKND
                     GSSDWMN"
     gene            774..1376
                     /locus_tag="VFI55_08670"
     CDS             774..1376
                     /locus_tag="VFI55_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7074136.1"
                     /translation="MRSPRERMVVSAALLIRERGAHATAISDVLHHSGAPRGSAYHYF
                     PGGRTQLLSEAVDYAGEHVGAIIAEAGGSLELLDTLIDTYRRQLLDSDFRAGCPVVAV
                     SVESGDEQDRERMVPVIERAAAVFDRWTGLIARRFIADGIQADQADELAVLATAALEG
                     AIVLARVRRDPTPLDLVHRQLRSSLLAALDTNEGNAGRDR"
     gene            1366..3636
                     /locus_tag="VFI55_08675"
     CDS             1366..3636
                     /locus_tag="VFI55_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7074137.1"
                     /translation="MTADWQSTACILCECNCGIVVQVEDRRLARIRGDKAHPGSQGYT
                     CNKALRLDHYQNSGARLTSPLRRRPDGSYQEIDWDTAIVEIAEGFKHIRDTYGGDKIF
                     YYGGGGQGNHLGGAYSGAFLKALGSRYRSNALAQEKTGEAWIDQQLYGGHTRGEFEHA
                     EVSVFVGKNPWMSQSFPRARVVLNEIAKDPARSMIVIDPVVTDTAKMADFHLRVRPGT
                     DAWCLAALAAILIQENLCDEAFLAAHVHGVDTIRAALREVAVTDYAQHCGVDDELLRA
                     AARRIATAATVSVFEDLGIQQAPNSTLCSYLNKLLWILTGNFAKKGGQHLHSSFAPLF
                     SQVSGRTPVTGAPIISGLIPSNVVPEEILTDHPDRLRAMIVESSNPAHSLANSAACRE
                     AFKALELMVVVDVAMTETARLAHYVLPAASQFEKPEATFFNFEFPANTFQLRRPLLQP
                     LPGTLPEPEIWARLVRALGVLDDTELRPLREAARRGRQDYAEAFLSAVASNPTVARLV
                     PYVLYETLGPTLPDGLSGAAALWGLAQKTAMSYPEAVARAGHANGDALFDAILSSPSG
                     VTFTVHNYDDDFALISHGDHKIALEIPEMTDELRALAATPPRLTTPEFPIVLSVGERR
                     AYTANDIFRDPSWRKRDADGALRVSVEDAQALGLVDGCRVRISTAAGSAEASIEITET
                     MLAGHAALPNGFGLDYVGDDGRTVVPGVAPNMLTSTEWRDPYAGTPWHKHVPARIELC
                     SRGEGTPAREEASSRS"
     gene            complement(3620..5608)
                     /locus_tag="VFI55_08680"
     CDS             complement(3620..5608)
                     /locus_tag="VFI55_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HET7074138.1"
                     /translation="MTLEAIQSGIDLSYVDADARPQDDLFGHVNGRWLAEYDMPPDRA
                     TDGAFRHLYDRAEEQVRDLITEASEKADLADSDADAQRIGDLYGSFLDEEAVERRGLQ
                     PLHDELTVIDGAADATALAAAVGALQRTGVGGGVAMYVDTDAKNSARYLVHFTQSGIG
                     LPDESYFRDEQHAEVLAAYPKHIARMFGLVFGGEASDHAETASRIVALETKLAAAHWD
                     VVKRRDADLSYNLRTFAELHSEAVGFDWAGWATALGSAPEALAEVVVRQPDYLTAFAA
                     LWGSEDFEDWKRWARWRLIRARAPWLTDELVAADFEFYGRLLTGAEQIRERWKRAVSL
                     VESLMGDAVGKLYVQRHFPPDAKARIDALVENLQAAYRISISDLDWMTPQTRERALTK
                     LSKFTAKVGYPARWRDYSKLVIDRNDLYGNYQRGHAVNHDRELAKLGGPVDRDEWFMT
                     PQTVNAYYNPGMNEIVFPAAILQPPFFDAEADDAANYGGIGAVIGHEIGHGFDDQGAK
                     YDGDGNLVDWWTDDDRTEFGVRTTALIEQYDEYTPRALAGHHVNGAFTVGENIGDLGG
                     LSIALLAYQLSLNGRPAPVIDGLTGVQRVFYGWAQVWRTKSREAEAIRRLAVDPHSPP
                     EFRCNGVIRNMDAFYDAFDVAEGDALYLAPRSRVKIWN"
     gene            5651..6313
                     /locus_tag="VFI55_08685"
     CDS             5651..6313
                     /locus_tag="VFI55_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015291965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074139.1"
                     /translation="MPDGERTKPATRDTKEESVEPDDDSGPGASGPEPGGGRMFSSYG
                     IASTVLGALSVAAVVLGAIIWSNHRAEVNERARLTRVMQTAADWAGVLINMNSGNVDA
                     SLQRLHDGTVGELNVDFDAAMQPYRQVVQKLQSQSAGRIDAVAVEAVHHDLDTQPGVA
                     RPVVTTKLPALATRTDSVMLVATSVSENIGVKPQTVHWNLRLDVSEVDGKLMISALES
                     IR"
     gene            6310..6999
                     /locus_tag="VFI55_08690"
     CDS             6310..6999
                     /locus_tag="VFI55_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074140.1"
                     /translation="MRNLWRLLAFDIVAPLAAVGALLMIGVVLDWPRWWVSTCSVLVL
                     LIVQGAAVNFWLLRRDSVSVGTDDDAPGLRLAVVVVCATAVSAAVLTEYTHWTSSDHE
                     FKEDSREVVRVASGLAEAMASFSPSSPTSSIDRAAAMMAPEQAGAFKEQYQKSSADLV
                     QHNITAQAATLSAGVEALGPSAASVAVILRVTQTSPGQPPSRAVPALRVALTKKGDNW
                     LVSDVTPINSR"
     gene            complement(7004..7534)
                     /locus_tag="VFI55_08695"
     CDS             complement(7004..7534)
                     /locus_tag="VFI55_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020731491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET7074141.1"
                     /translation="MTLAADRRPAPPPQRPAGGQRKPADGTDQAPDQPVELWPTVAIR
                     SALQGGDIATWKRIAAALKRDPYGRTARQVEEVLEGTRPYGISKALWEVLERARAHLD
                     ANERAEVARHVRLLIDRSGLAEQEFASRIGVSPQDLAAYLDGSASPSAALMIRIRRLS
                     DRFVKVKSARSGESNN"
     gene            complement(7531..10617)
                     /locus_tag="VFI55_08700"
     CDS             complement(7531..10617)
                     /locus_tag="VFI55_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET7074142.1"
                     /translation="MMRLSRSLRRYRWLVFTGWLLALVPAIYLAMTQSGNLTGGGFEV
                     AGSQSLAVHDQLEDLYHDQGASSLALVAAPRPDASYQDINDAVAQLRQIAGEFPNVTE
                     VPNPTQRPPQPDRPYVVSLRLDAHNAGTSDLAKQLRKKVGVKGDQSGQTANGRVRLYV
                     IGQGALSAAAAENTKRDIAAAERWNLPIILIVLLAVFGSLAAAAIPLALGVCTVVVTM
                     GLVYFLSAYTTMSVFVTSTVSMFGIALAVDYSLFILMRFREELRTGRRPNEAVDAAMA
                     TSGLAVVLSGMTVVASLTGIYLINTPALKSMATGAILAVAVAMLTSATLTPAVLATFG
                     RSAAKRSALLHWSRRPESTQSRFWNRWIGSVMRRPWISAMAAAVVLLVMAVPAASMVL
                     GNSLLRQFDSSHEIRAGVAAAAQALGPGALGPIRVLITFPDGGAASPEHSQTIAAIRQ
                     RMTQAPNIVSVSPPQFAEDNGSALLSAVLSVDPEDMGARDTVGWMRTQLPKIPDAGTA
                     RVDVGGPTALIKDFDDRVSQTEPLVLLFVALIAFVMLLISIHSVFLAFKGVLMTLLSV
                     AAAYGSLVMVFQWGWLKDLGFTQISSIDSTVPPLVLAMTFGLSMDYEIFLLTRIRERF
                     LHSGNTRDAVAYGVSTSARTITSAALIMIAVFIGFAFAGMPLVAEIGVACAVAIAVDA
                     TVVRLVLVPALMAMFTQWNWWLPGWLRRILPSVDFDRPLPEVDLADVVVIPDDISALI
                     APGADLRMVLKSAAKLRHLAPDAICVADPLAFTGCGAGDKEPETGSAEDRGNGAQARG
                     RGPGQIPHQVNITDDAELVAVGPAGTGSSAKTGTNGQTRGQSGAKKLVGGLASRNGIA
                     RAIAGGDRPVHPVTLWRGRLSVAIDALEAHRVSNADNTEDLKYERRSPVETTHVQLPT
                     GDRLLIPTGAEALRLKGYLIMCRNSSRDYAEFADMVETVQPETAAQVLGAMDTYYCCQ
                     TPRRQWIATQLVRRLADPHPCDFRDDKFSEADAKADWDQVKQRCLSVAVAMLEEAR"
     gene            complement(10942..12180)
                     /locus_tag="VFI55_08705"
     CDS             complement(10942..12180)
                     /locus_tag="VFI55_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HET7074143.1"
                     /translation="MSHDALTRKLHLARAGAGPRVPVGAPHAGGVGPPAREENPRGKY
                     WWVRWVILGVVAIVLAVEIALGWDQLAKAWVSLYEANWWWLLAAVLAAAASMHSFAQI
                     QRTLLRSAGVHVKQLRSEAAFYAANSLSTTLPGGPVLSTTFLFRQQRMWGASTVVASW
                     QLVMSGVLQAVGLALLGLGGAFFLGAKNNPFSLLFTLGGFVALLLLAQAVASRPELIE
                     GIGTRVLAWVNSVRGKPADTGLDKWRETLMQLESVSLSRRDLGVAFSWSMFNWIADVA
                     CLGFAAYAAGDHASVAGLTVAYAAARAVGTIPLMPGGLLIVEAVLVPGLVSSGMSLPN
                     AISAMLLYRLISWLLIAAIGWVVFFFMFRTENATDSDDDPITGPLPRVQLQGDHFADP
                     TETALQGPVPPPEQIPPSDG"
     gene            12361..13509
                     /locus_tag="VFI55_08710"
     CDS             12361..13509
                     /locus_tag="VFI55_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7074144.1"
                     /translation="MSANLDDTAVEPLVRKAAAWAWRLLIILTAAVALLWVIDKLEIV
                     VVPVLLALIISALLVPAVDWIDRWGLPRGAAVALVLLVGFAILGGILAFVIIQFIDGL
                     PDLTEQVTRSIDSIRKWLIEGPLHVRGEQIGNAGNAAIQALHNNQAKLTSGALATAAT
                     VTELVTAAVLVLFTLIFFLYGGRNIWHYVTKIFPVSVRERVREAGRAGYRSLIAYIRA
                     TFLVALTDAAGVGTGLAIMGIPLALPLASLVFLGAFIPLVGAVIAGFLAVIVALLAKG
                     FLYALITVGLLIAVNQLEAHLLQPLVMGRAVSIHPLAVVLAISTGGVLAGIVGALLAV
                     PTVAFLNNAIQVLLAPDPSAEVEKQTEGEDDFGVMVQAKADDPSDDPN"
     gene            complement(13530..16421)
                     /locus_tag="VFI55_08715"
     CDS             complement(13530..16421)
                     /locus_tag="VFI55_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET7074145.1"
                     /translation="MFAWWGRTVYRYRYIVIGVTVALCLIGGAFGLSLGKHVTQSGFY
                     DDGSQSVKASIIGDKVYGRDRTGHIVAIFKAPDGKNVTDPAWAKQITDEINKFQQDHP
                     DQVLGWAGYLRAPDSPSSVIQGMVTPDKKYTFVTIPLKGNDDDSILNNYKTIAPDLQK
                     LDGGTVQLAGLEPVANALTGTIATDQRRMEVLALPLVAVVLFLVFGGVIAACLPVMVG
                     GLSIAGSLGILRLIAVFGPVHFFAQPVVSLIGLGIAVDYGLFVVSRFREEIAEGYDTE
                     TAVRRTVMTAGRTVTFSAVLIAASGASLLLLPQGFVKSLTYALIAAVTLAAVLSITLL
                     PACLGILGKNVDALGVRTLFRVPFLRNWWVSRGYLNWLADRLQKTKTREEVEAGFWGK
                     LVNFVMKRPLTFAIPIVIGMILLVIPLGNLSFGGMSEKYLPPNNPVRQAQEHFDQLFP
                     GYRTNPLTLVIQSTNHQPVTDQQVADIRSKAMAISGFSDQDNNPANMWQERSYAPDAS
                     KDPAVRVLQNGLINPNDAAKKINDLRSITPPKGLTLWVGGTPALEQDSIHSLVARAPL
                     MVLVLITTTMLLMFLAFGSFVLPVKAAVMSALTLGSTMGILTWIFVDGHFSKWLNFTP
                     TPLMVVIIALVVAVGYGLATDYEVFLVSRMVEAREEGMSTQEAVRIGTATTGRLITAA
                     ALVLAVVAGSFVFSDLVMMKYLAFGLMAALLLDATVVRMFLVPSVMKLLGDDCWWAPR
                     WARRLQNRIGLGEIDLPDERKRPTLNGRPSRPPVVTPSLVAATARPAHDPTHPGALEP
                     PRPVRSGPPEPRPAAELPAPSGASTARIQTGPSQPTASTTRHSVPANTNAVPGGQAAP
                     GRQRPEDRRPGSSTPPAPPRAQAPPSPPTPSSGQTRAMPVPTSRSDDDKADPADPTAA
                     LPVMRSDANDSDAATEKLNARGQSDNGDRRRRGGGGLSAQDLLRREGRL"
     gene            complement(16471..17184)
                     /locus_tag="VFI55_08720"
     CDS             complement(16471..17184)
                     /locus_tag="VFI55_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET7074146.1"
                     /translation="MSLEETAAASQPLASPGVPSGAALASLSPAPGGRVLLVWDAPNL
                     DMGLGSILGRRPTAMERPRFDALGRWLLTRTAEVSAGHPGAVVEPEATVFTNIAPGSA
                     DVVRPWVDALRNVGFAVFAKPKIDEDSDVDRDMLAHIELRRTEGLAALIVASADGQAF
                     RQPLEEIARDGIGVQVIGFREHASWALASDTLDFVDLEDIDGVFREPLPRIGLDSLPD
                     QGAWLQPFRPLSALLTARV"
     gene            complement(17212..18054)
                     /gene="trmB"
                     /locus_tag="VFI55_08725"
     CDS             complement(17212..18054)
                     /gene="trmB"
                     /locus_tag="VFI55_08725"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET7074147.1"
                     /translation="MGHHGQMHSQPGVGTCPDTPVEVGFGRQAGRATGEGMTAPLAPD
                     PDRPEPGYLPATGFRSRRSALSGAQRETWERLWPELGISAGTASGGREGAEPLDTGVW
                     FGRVAPVVLEIGCGSGTSTLAMAKDEPGIDVIAVEVYRRGLAQLLCAMDRERVGNIRL
                     IRGNAIDVLAHRIAPGSLTGVRVFFPDPWPKARHHKRRFLQPATIGLIADRLLPGGVL
                     HAATDHPGYAEHIAEAGDGEPRLRRADADSRLPISVVRPTTKYETKAHDAGNSVTEFV
                     WRRL"
     gene            18276..19430
                     /locus_tag="VFI55_08730"
     CDS             18276..19430
                     /locus_tag="VFI55_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074148.1"
                     /translation="MTRRGHQIFAEELAGFAAGHADPRVTAIAERAAAPLRVLVCGRG
                     GVGRSTVAHALDRAGGALGISATTGATPPTCAGDSAPALGEIFDEISADVVVYVTAEV
                     VKPEDIDAIGTAGPPVLAVLNKADLAGSLSGRAGDEPMVAARMRCADLSALVGVPMEP
                     MIGLLAVAALDDLDGALWAALRVLAADPDGATCLDGSFAGFLDADVAVPTDVRLRLLD
                     TLDLLGTALGIAAVQQGRTPAQLRALLRRMSGVDAVLTAVAALGAEVRYQRMLQVVAE
                     LEALAVGQGDLGESISGFLSCDDTVVARMAVAVDLAEAAGLGPIDLAPGNLDRDDPAA
                     HLPRAVHWQRYSLGRLGPVSDLHRLCGTDIARGSLRLWSQTCGSLPGESW"
     gene            19427..20959
                     /locus_tag="VFI55_08735"
     CDS             19427..20959
                     /locus_tag="VFI55_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074149.1"
                     /translation="MTPKAAGDPDTQVDALVAAIGPRLEVPAINRRDVVLVTGPWMAG
                     VSSVVGVLRERLPRHTFVESTDLATGDAPIAAVFVVSAAAQLTGSDCAQLDAAAENTD
                     VVVGVVSKIDVHRNWRDVVAADHDILADHAPRYAKVPWVGVAAAPDLGEPIVDELVET
                     VAARLAEPDIARRNRLRAWDSRLQTVARRFDRDAEGSGRRARVDTLREERSTALRQRR
                     QSKTERTVTLRGQIQQARVQLSYFARNRCSSVRTELQEDAADLSRRNMSRFEAHTRGR
                     VDEVVAEVSEGTSTQLVDVAHVMGVPMALPALEKLPGVEVSPPPLKSRRHETWLMMLL
                     GAGFGLGVALTLGRLVGGLASRLSPGLAIAGVAGCVVIGLAVTFVVIHIRGLLRDRAL
                     LDRWAGDVTSSLRSVVEELVATRVLVAESVLSSALIARDEAENTQVADQVSAIDSELR
                     EHAIAAARAAAVRDREMPAVQAALEAVRAELGESGVSQSGNSASDRSEEKNDKPGLDE
                     DE"
     gene            21147..22976
                     /locus_tag="VFI55_08740"
     CDS             21147..22976
                     /locus_tag="VFI55_08740"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HET7074150.1"
                     /translation="MTSATIPGLDTAPTNHPGLLSWVEEVAELTQPDRVVFADGSDEE
                     CERLSAQLVEAGTFTKLNEKKHPNSYLALSDPSDVARVESRTFICSRREIDAGPTNNW
                     MDPAEMRSIMTDLYRGCMRGRTMYVVPFCMGPLGADDPKLGVEITDSEYVVVSMRTMT
                     RMGKAALEKMGDDGFFVKALHSVGAPLEPGQEDVPWPCNDTKYITHFPETREIWSYGS
                     GYGGNALLGKKCYSLRIASAMAHDEGWLAEHMLILKLISPENKAYYFAAAFPSACGKT
                     NLAMLQPTIPGWRAETLGDDIAWMRFGKDGRLYAVNPEFGFFGVAPGTNWKSNPNAMR
                     TIAAGNTVFTNVALTDDNDVWWEGLEGDPQHLIDWKGNDWYFRETETNAAHPNSRYCT
                     PMSQCPILAPEWDDPQGVPISGILFGGRRKTTVPLVTQARDWQHGVFIGATLGSEQTA
                     AAEGKVGTVRRDPMAMLPFLGYNVGDYFQHWIDLGKNSDESKLPKVFFVNWFRRGDDG
                     RFLWPGFGENSRVLKWIVDRIEHKAGGQDTPIGIVPTAGDLDLDGLDVDGADVEKALV
                     VNADEWRKELPLIEEWFEFVGDKLPTGVKDEFEALKQRLSDAD"
     gene            complement(22997..23479)
                     /gene="soxR"
                     /locus_tag="VFI55_08745"
     CDS             complement(22997..23479)
                     /gene="soxR"
                     /locus_tag="VFI55_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HET7074151.1"
                     /translation="MDKRDLFTVSEIAHRSGFAASAIRFYESQGLISATRTAGGQRRF
                     ERQTLRRLAFIRAARNVGLSLDEVADALAKLPQSRTPTRADWAGLSKSWRTRLDDQIA
                     GLIALRDNLDSCIGCGCLSLKRCAISNPGDSAAVAGSGAVYLPKSLRRQLISRSPGAG
                     "
     gene            23539..24270
                     /locus_tag="VFI55_08750"
     CDS             23539..24270
                     /locus_tag="VFI55_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7074152.1"
                     /translation="MTRSQPHPVAIVTGASRGFGKAVTAALLERGWVVVGDARRAVEL
                     KTTAAELNSTRLIALPGDITDASHRAALVAAALRAGPLRLLVNNASRLGPSPQPALAD
                     YPPNQLWAVYQTNVLAPLALIQATLPALADNAGVIINVTSDAAVEPYPGWGGYGSSKA
                     ALDQLSAVLAAEVSVTSAAPAVAVYAFDPGDMRTEMHQAAFPGEDISDRAEPEAKVPA
                     LLRLLDTRPAAGRYRAADLTTAGAR"
     gene            24270..25373
                     /locus_tag="VFI55_08755"
     CDS             24270..25373
                     /locus_tag="VFI55_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HET7074153.1"
                     /translation="MTLAMSPQTHFHRPPGSDAFEPPEAHGVARDAVKLLVARPGGVT
                     HTRFADLGAQLRPGDVLVVNNSATLPAAVDGRRAGRLIAVHFSMARAEKLWVVEPRLA
                     DTAEHLTDIQPGERIDLPGGAALVIGSSYPEPGLGGARLWTARVILEGPAGAVTSYLG
                     RYGRPIRYSYVPRAWPLRYYQTVFARSAGSAEMPSAARPFTTQLVTALVAAGIAIAPI
                     TLHAGVSSAEAGEPPTPEPFAVPAATARMVNSAHAAGSRVIAVGTTATRALESAANAD
                     GVVRPAAGWTDLVLGPGRPCRVVDGLITGWHDAGASHLLLLEAAAGPELVAMAYREAV
                     EHGYLWHEFGDSALLLPGRGYRPPSTTGRQAPA"
     gene            25407..26948
                     /gene="fadD4"
                     /locus_tag="VFI55_08760"
     CDS             25407..26948
                     /gene="fadD4"
                     /locus_tag="VFI55_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid--CoA ligase FadD4"
                     /protein_id="HET7074154.1"
                     /translation="MQIRPYTSGRGGANKPAVILHPSGTVITFDDLEARANRLAHRFR
                     QAGLREGDTVAILMENNEHIHAVMWAARRSGLYYVPINTHLTAAEAAYIVDNSGAKAI
                     IASAALLDTCAGLADYLPNGLPDLLMLASGTEDADLPGWERYPECVAGQPDTPIDDER
                     EGDLLQYSSGTTGRPKGIKRQLPHTSPAESPGMMSALVNFWMTPESIYLSPAPLYHTA
                     PSVWSMSAQAGGVTTVVMEKFDPEGTLDAIGRYQVTHGQFVPAMFTRMLKLPEAVRDS
                     YDVSSLQRVMHAAAPCPVQIKKEMIDWWGPIVDEYYASSEAIGSTLISAEEWLAHPGS
                     VGKPMMCQIHIVGEDGNELPPGQPGEIYFEGGYSFEYLNDPSKTAASREKHGWTTVGD
                     VGYLDDEGYLYLTDRRHHMIISGGVNIYPQEAENLLVTHPKVLDAAVFGVPDDEMGQR
                     VVAAVQTVDPADATDQFADELIGWLRDRLSHFKCPRSIAFETQLPRTDTGKLYKNALI
                     EKYSV"
BASE COUNT         4418 a         9041 c         9127 g         4429 t
ORIGIN      
        1 ctaatgggtc gacggtgggg gttggccagc cgatcatcct caacttcccg ggaacggtcg
       61 aggacgccgg tgccgcgata aacgccgtgc acgtgtcgtc ggtcccgccc gttcccggca
      121 agttctactg gatgacgccg actcagctgc gctggcggcc cttgagcttc tggcccgccc
      181 acaccgcggt gaccgtcgac gccggcggta ctgtgtccag cttccagacc ggggactcgc
      241 tgatcgccac ggcggacgac tccacgcacc agctgaccat cacccgcaac ggcaccgtgg
      301 agaagacctt ccccatgtca atggggatgt cggcgggcaa ccaccaaacg cccaacggca
      361 cctactacgt gcaagagaag atgccctcgg tggtgatgga ttcctcgacg tacggggttc
      421 ccgtcaactc gacgtacggc tacaaggtga ccgtcgaact ggccgtccgg ttcgacaacg
      481 tcggtgactt cgtgcacagc gccccgtggt cggtggacga ccagggcaag cgcgacgtca
      541 gccacggctg catcaacatc agccccacga acgcgaagtg gttcttcgac aacttcggcc
      601 ccggtgatcc gatcatcgtg aagaactcca ccggcggcga ttacaagaag aacgacggat
      661 ccagcgactg gatgaactaa cggttcacgg cgggacgctc accaaggctt ccggctgtcg
      721 ggcggttatg tataccatca tacataagcc ctgcggcaac tgaggagcgt ttcatgcgta
      781 gcccacgcga gcggatggtg gtttctgctg cgctgttgat ccgcgagcgc ggcgcccatg
      841 ccaccgccat ctcggacgtc ctgcaccaca gcggagcgcc acgcggttcg gcctatcact
      901 acttcccggg cgggcgaacg caactgctct ctgaggccgt cgactacgcg ggcgagcacg
      961 tcggcgccat catcgccgag gccgggggaa gcctcgaact gctggacacg ctgatcgaca
     1021 cctaccggcg tcagttgctc gacagcgact tccgcgcggg ctgcccggtc gtggcggtct
     1081 ccgtcgaatc gggtgacgag caggaccgcg agcggatggt cccggtgatc gagcgcgccg
     1141 cggcagtgtt cgaccgctgg accggcctga tcgcgcggcg cttcatcgcc gacggtatcc
     1201 aggcggatca ggccgacgaa ttggcggtgt tggcaaccgc cgcactcgaa ggcgccatcg
     1261 tgctggcgcg ggtgcgacgc gacccgacgc cgctggacct cgttcaccgc cagctgcgta
     1321 gctcgctgct ggctgccctg gacacgaacg aagggaacgc cggccgtgac cgctgattgg
     1381 cagtccaccg catgcattct gtgcgaatgc aattgcggca tcgtcgtgca ggtcgaagat
     1441 cgccggctgg cgcgcatccg gggcgacaag gcgcacccgg ggtcgcaggg gtatacctgc
     1501 aacaaggcgt tgcggctgga tcactaccag aacagcggcg cccgattgac ctctccactg
     1561 cgccgccgcc ccgacggcag ctaccaggag atcgactggg acacggcgat tgtcgagatc
     1621 gccgaggggt tcaagcacat ccgtgacacc tacggtggcg acaaaatctt ctactacggc
     1681 ggcggcgggc agggcaacca cctgggtggg gcgtacagcg gcgccttcct gaaggccctg
     1741 ggatcgcgat accggtcaaa tgcgctggcg caggagaaga ccggtgaagc gtggatcgat
     1801 cagcagctgt acggcggcca cacgcgtggc gagttcgagc acgccgaagt gtcggtgttc
     1861 gtcgggaaga atccatggat gtcgcagagc ttcccccgag ctcgggtggt actcaacgag
     1921 atcgccaaag atccggcccg gtcgatgatc gtgatcgatc ccgttgtcac cgacaccgcg
     1981 aagatggccg actttcatct gcgggtgcgg cccgggaccg atgcctggtg tctggcggcg
     2041 ctcgccgcca tcctcatcca ggaaaacctt tgcgacgaag catttctcgc cgcgcatgtg
     2101 cacggtgtcg acactatccg cgccgcgctg cgcgaggtcg cggtcaccga ctacgcgcag
     2161 cactgcggcg tggacgatga gctgctgcgc gccgcggcgc ggcgcatcgc caccgccgcc
     2221 accgtctcgg tgttcgagga cctcggcatt cagcaggcac caaacagcac cctgtgctcc
     2281 tacctcaaca agctgttgtg gattctgacc ggtaacttcg cgaaaaaggg tggccagcac
     2341 ctgcattcgt cgtttgcccc gctgttcagt caggtctccg gccgcacacc ggtcaccggc
     2401 gccccgatca tttccggtct gattccgagc aatgtcgtgc ctgaggagat cctgaccgat
     2461 catccggacc gcttgcgggc gatgatcgtc gagagcagca atcccgccca ctcgctggcc
     2521 aactcggcgg cctgccggga ggcgttcaaa gcgctggaac tgatggtggt cgttgacgtc
     2581 gcgatgaccg agaccgccag gctcgcccat tacgtgctac ccgccgcctc tcagttcgag
     2641 aagccggaag cgacgttctt caatttcgag ttcccggcca acacttttca gctgcgccgg
     2701 ccattgctgc agccgttgcc ggggacgttg cccgaaccgg agatctgggc ccggctggtg
     2761 cgcgcgctcg gcgtgctcga cgacaccgag ctgcggccgc tacgcgaggc cgcccggcgc
     2821 ggccgccaag actatgccga agcgtttctc agcgccgtgg cgagcaatcc gaccgtggcc
     2881 agactggttc cctatgtcct ctacgaaaca ctggggccga cgctgccgga cgggttgtcc
     2941 ggtgcggcgg ccctgtgggg actcgcccag aagaccgcca tgagctatcc cgaagccgtt
     3001 gcgcgtgccg gccacgccaa cggcgacgca ctgttcgacg ccatcctcag cagcccgtcc
     3061 ggcgtcacgt tcaccgtgca caactatgac gacgatttcg cgctgatcag ccacggcgac
     3121 cacaagatcg cgctggaaat ccccgaaatg accgacgagc taagggcgtt ggccgctaca
     3181 ccgccccggc tgacgacgcc ggagtttccc atcgtgctgt cggtgggcga gcggcgtgcc
     3241 tacaccgcca acgacatctt ccgcgacccg tcatggcgca agcgggacgc cgacggggca
     3301 ttgcgggtca gcgtggagga tgcccaggcc ctggggctcg tcgacggatg ccgggtccgc
     3361 atcagcaccg cggccggcag cgccgaggcg agcatcgaaa tcaccgagac catgctggcc
     3421 ggacatgcgg ccctgcccaa cggtttcggg ctggactacg tcggcgacga cgggcgcacc
     3481 gtcgttccgg gcgtcgcccc gaacatgctc acctccaccg aatggcgcga cccgtacgcc
     3541 ggcacaccgt ggcacaaaca tgtgcccgcg cggatcgaac tgtgctcgcg cggggaaggt
     3601 accccagctc gcgaggaagc tagttccaga tcttgaccct actcctgggc gccaggtaca
     3661 acgcgtcgcc ctcggccacg tcgaaggcgt cgtagaaggc gtccatgttg cggatgacgc
     3721 cgttgcaccg gaactccggc ggggaatgtg ggtcgaccgc cagccgccgg atcgcttctg
     3781 cctcacgtga tttggttcgc cacacctgcg cccatccgta gaacacccgc tgcacgccgg
     3841 tgaggccgtc gatgacgggg gcgggccggc cgttcaggga cagctggtag gccaatagcg
     3901 cgatggacag cccgcccagg tcgccgatgt tctcgccgac ggtgaatgcg ccattcacgt
     3961 gatgcccggc gagcgccctg ggtgtgtatt cgtcgtactg ctcgatgagc gccgtggtgc
     4021 ggacaccgaa ttcggtgcgg tcgtcgtcgg tccaccagtc gaccaggttg ccgtcgccgt
     4081 cgtacttggc gccctggtcg tcgaaaccgt gcccgatctc gtgtccgatc accgcgccga
     4141 tcccgccgta gttggcggcg tcgtcggcct cggcgtcgaa gaacggtggt tgcaaaattg
     4201 ctgcgggaaa cacgatttcg ttcatcccgg ggttgtaata ggcgttgacg gtctgcggcg
     4261 tcatgaacca ctcgtcgcgg tccaccgggc cgccgagctt ggccagctcg cggtcgtggt
     4321 tgaccgcgtg cccgcgttgg tagttgccgt acaggtcgtt gcggtcgatc accagcttcg
     4381 agtagtcgcg ccacctcgcc ggatagccga ccttggcggt gaacttgctc agcttcgtca
     4441 gcgcgcgctc ccgggtctgc ggcgtcatcc agtccagatc gctgatgctg atccgatacg
     4501 cggcctgcag gttctccacc agcgcgtcga tccgcgcctt ggcgtcgggc gggaaatgcc
     4561 tttgcacgta aagctttccg acggcgtcgc ccatcaggct ctccaccagg gacacggccc
     4621 gcttccagcg ttcgcggatc tgctcggccc cggtcaacag gcggccgtag aactcgaagt
     4681 ccgcggcaac caactcgtcg gtcagccaag gcgcacgggc ccggatcaaa cgccaacgcg
     4741 cccagcgctt ccagtcctcg aagtcctcgc tgccccagag tgcagcgaac gcggtgaggt
     4801 aatccggttg gcgcacaacc acttccgcga gagcctccgg tgcgctgccc agcgcggtcg
     4861 cccagccggc ccagtcgaag cccaccgcct cgctgtgtag ctccgcgaag gtgcgcaggt
     4921 tgtagctgag gtcggcatcg cggcgcttaa cgacatccca atgcgcggca gcgagtttgg
     4981 tctccaacgc cacgatccgg gacgcggtct cggcgtgatc gctcgcttcc ccgccgaaga
     5041 ccaggccgaa catccgcgcg atgtgcttcg gataggccgc cagcacctcg gcgtgctgct
     5101 cgtcgcggaa atacgattcg tcgggcagcc cgatgcctga ctgggtgaag tgcaccagat
     5161 aacgagccga gttcttggcg tcggtgtcca catacatcgc cacgccgccg ccgacccccg
     5221 tgcgttgcag ggcgcccacc gcggcggcca acgccgtggc gtcggcggcg ccgtcgatga
     5281 ccgtcagctc gtcgtgcagc ggttgcagtc ccctgcgttc gacggcttcc tcgtcgagga
     5341 agctgccata gaggtcgccg atgcgttgcg cgtccgcgtc cgagtccgcc aggtccgctt
     5401 tttcgctggc ttcggtgatc aggtcgcgca cctgctcttc ggcgcggtcg tagaggtggc
     5461 ggaaggcgcc gtcggtcgcc cggtccggcg gcatgtcgta ttcggccagc cagcggccgt
     5521 tgacatgtcc gaataggtcg tcttgagggc gggcgtccgc gtcgacatag ctcaggtcga
     5581 tgcccgattg gatggcctct agcgtcaccc cgccatcctt ccatctcctt ttgggtgcga
     5641 cgatcgggcc atgcccgacg gcgagcgaac caagccagcg acccgcgaca caaaagaaga
     5701 gtccgtcgag cctgacgatg actccggccc cggcgcttcc gggcccgaac ctgggggcgg
     5761 ccggatgttc tccagttatg gcatcgcgtc gaccgtgctg ggcgcgctgt cggtcgccgc
     5821 ggtcgtgctc ggcgcgatca tctggtcgaa tcaccgtgcc gaggtgaacg aacgcgcccg
     5881 cctcactcgc gtcatgcaga ccgccgccga ctgggcgggc gtgctgatca acatgaacag
     5941 cggcaacgtc gacgccagct tgcagcggct gcatgacggg acggtcggcg aactcaacgt
     6001 cgacttcgac gcggccatgc agccctaccg gcaggtggta cagaagctgc agtcgcagag
     6061 tgccggccgg atcgatgcgg tagcggtcga agcggtacac catgacctgg acacccagcc
     6121 cggtgttgcc cggcccgtgg tcaccaccaa gttgccggcg ttagccaccc gaacggactc
     6181 ggtgatgctg gtcgcgacgt cggtcagcga gaacatcggc gtcaagccgc agacggtgca
     6241 ctggaacttg cggctcgacg tctccgaggt ggacggcaaa ctgatgatct ccgcgctgga
     6301 atccatccga tgagaaacct ctggcggctc ctggcgttcg acatcgtggc gccactggcc
     6361 gcggtcggcg cactgctgat gatcggagtg gtactcgact ggccgcgatg gtgggtgtcg
     6421 acgtgctcgg tgctggtgct gctgatcgtc caaggcgctg cggtcaactt ctggctgctg
     6481 cgccgcgatt cggtcagcgt cggtaccgac gacgacgcac cggggctgcg attggccgtt
     6541 gtcgttgtct gcgcgaccgc cgtgtcggcg gcggtgttga ccgagtacac gcattggacg
     6601 agctcggacc acgagttcaa agaggattcc cgcgaggtgg ttcgggtcgc ctccggcctg
     6661 gccgaggcga tggcgtcgtt cagcccgagc tccccgacga gctccatcga ccgggccgcc
     6721 gcgatgatgg cgccggaaca ggcgggcgcg ttcaaggagc agtaccagaa gtccagcgcc
     6781 gacctggtcc aacacaacat cacggcgcag gccgccacgc tgtcggccgg ggtcgaggcc
     6841 ctcgggccgt cggcggccag cgtggcggtg atcctgcggg taacccagac ctcgcccggg
     6901 cagccgccca gtcgggcggt gcctgcgcta cgcgtcgcgc tgaccaagaa gggcgacaac
     6961 tggctggtgt cggacgtgac gccgatcaat tcccgctgac ggatcagttg ttggattcgc
     7021 ccgagcgtgc cgacttcacc ttgacgaacc ggtccgacag ccggcggatc cggatcatca
     7081 gcgcggccga cgggctggcg ctgccgtcga gataggccgc cagatcctgg ggggacaccc
     7141 cgatgcgcga cgcgaattcc tgctcggcca ggccggaccg gtcgatcagg agccgcacgt
     7201 ggcgggccac ctccgcgcgc tcgttggcgt ccaggtgggc gcgggcgcgc tccagcacct
     7261 cccacagggc cttggagatg ccgtagggcc gcgtcccctc gaggacctct tcgacctgac
     7321 gggcggtgcg cccatagggg tcgcgtttca gggcggcggc gatgcgcttc caggtggcga
     7381 tgtcaccgcc ctgcagcgcc gaacggatgg cgacggtcgg ccatagctcc accggttggt
     7441 cgggtgcttg gtcggttccg tcggcaggtt tgcgctgtcc gcccgcgggg cgctggggcg
     7501 gcggtgccgg gcgtcggtcg gctgccaacg tcacctcgcc tcctccagca tcgccacggc
     7561 caccgacagg cagcgctgct tgacctggtc ccagtccgcc ttcgcgtcgg cttccgaaaa
     7621 cttatcgtcg cggaaatcgc aaggatgtgg atccgcgagc cgacggacca actgcgtggc
     7681 gatccattgc cgcctgggtg tttgacaaca gtaatacgtg tccattgcac ccagcacctg
     7741 cgcagcggtc tcgggctgca ccgtttcgac catgtcagca aattcggcgt agtcgcggct
     7801 gctgttacgg cacatgatca ggtagccctt gaggcggagc gcttcggcac cggtcgggat
     7861 gagtaatcga tcgccggtgg gcaattgcac gtgcgtggtt tcgaccgggc tgcgccgctc
     7921 gtatttgagg tcctcggtgt tgtcggcgtt ggagactctg tgcgcttcca gcgcgtcgat
     7981 ggcgaccgac agccggccac gccacaacgt gaccggatga accggccgat cccccccggc
     8041 gatcgcgcgg gcgatgccgt tgcgggatgc cagcccgccg acaagcttct tggccccgga
     8101 ctgacccctg gtctgaccgt tggtgccggt cttggcagaa gatcccgtgc ccgccgggcc
     8161 caccgcgacc agttcggcgt catcggtgat gttcacctgg tggggaatct gcccggggcc
     8221 acggccgcgc gcctgtgcgc cgttcccgcg gtcttctgcc gagccggttt cgggctcctt
     8281 gtcgccggcc ccacagccgg tgaaggcgag cgggtcggcc acgcagatgg cgtcgggcgc
     8341 caagtgccgc agcttcgcgg ccgacttgag caccatccgc aggtcggcac cgggcgcgat
     8401 cagtgccgag atgtcgtcgg ggatcaccac gacgtccgcg aggtcgacct cgggaagcgg
     8461 cctgtcgaag tcgaccgacg gcaagatccg gcgcagccat cccggcagcc accagttcca
     8521 ctgggtgaac atcgccatca gcgccgggac caggaccagt cgcaccaccg tggcgtccac
     8581 cgcgatcgcg acggcgcacg ccacgccgat ctcggcgacc agcggcatgc cggcgaacgc
     8641 gaagccgatg aacaccgcga tcatgatcag ggcggcgctg gtgatcgtgc gggcgctggt
     8701 gctcacgccg tatgcgaccg catcgcgagt attccccgag tgcaggaagc gttcccggat
     8761 gcgggtgagc aggaagatct cgtagtccat cgacagcccg aaggtcatcg ccagcactaa
     8821 cggtggaacg gtgctgtcga tcgagctgat ctgggtgaag ccgagatcct tcaaccagcc
     8881 ccactggaag accatcacca ggctgccgta ggcggccgcg accgacagca acgtcatcag
     8941 cacgcccttg aacgccagga acaccgagtg gatcgagatc aacagcatca cgaacgcgat
     9001 cagtgcgacg aagagcagca ccagcggctc ggtctgcgac acccggtcgt cgaagtcctt
     9061 gatcagcgcg gtcgggccgc cgacgtcgac tcgtgccgtt cccgcgtcgg gaatcttggg
     9121 cagctgggtg cgcatccagc cgaccgtgtc acgggcgccc atgtcttcgg ggtcgaccga
     9181 cagcaccgcg gacagcagag cgctgccgtt gtcctcggcg aattgcggcg gtgacaccga
     9241 gacgatgttg ggggcctgcg tcatccgctg acggatcgcc gcgatggtct ggctgtgctc
     9301 gggcgatgcc gcgccgccgt cggggaaggt gatcagcacg cgaatcgggc ccagcgcgcc
     9361 gggccccagc gcctgggcgg ccgcggccac cccggcgcgg atctcgtgcg acgagtcgaa
     9421 ctggcgcagc agactgttgc ccagcaccat cgacgcggcc ggcacggcca tgacgagcag
     9481 caccaccgcc gccgccatcg ccgatatcca cggccggcgc atcaccgatc cgatccagcg
     9541 attccagaat cgggactgcg tgctctccgg ccggcgcgac cagtgcaaca acgcggacct
     9601 cttggcggcc gaccggccga acgtcgccaa taccgccggg gtcaaggtgg ccgacgtcag
     9661 catcgccacc gcgacggcca gaatcgctcc ggtggccatc gacttcagtg ccggggtgtt
     9721 gatcaggtag atcccggtga gggaggcgac gaccgtcatc cccgacaaca ccaccgcgag
     9781 cccggacgtg gccatcgcgg cgtccacggc ttcgttcggc cggcggccgg tgcgtagttc
     9841 ctcgcggaag cgcatcaaga tgaacagcga atagtcgacg gccagcgcga tcccgaacat
     9901 cgacaccgtc gaggtcacga acaccgacat ggtggtgtac gcggacaaga agtagaccag
     9961 gcccatggtg accacgaccg tgcatacgcc cagggcgagc gggatcgccg ccgcggcgag
    10021 cgaaccgaac accgccagca agacgatcaa aatgatcggc aggttccagc gttcggccgc
    10081 ggcgatgtcg cgcttggtgt tttccgccgc ggcggcgctc aacgcgccct gcccgatgac
    10141 gtaaagccgc actctcccgt tggcggtctg accggattga tcgcccttga cgccaacctt
    10201 tttgcggagc tgcttggcga ggtcactggt gccggcgttg tgggcgtcca gccgcagtga
    10261 caccacatac ggccggtccg gttgcggtgg ccgctgggtg gggttgggca cctcagtcac
    10321 gttggggaac tcgccggcga tctgccgcag ctgggcgacc gcgtcgttga tgtcctggta
    10381 gctggcgtcc gggcggggag ccgccaccag cgccagcgag gacgctccct ggtcgtggta
    10441 gaggtcctcg agttgatcgt ggacggccaa cgactgcgag ccggcgactt cgaaaccacc
    10501 gccggtgaga tttccggact gtgtcatcgc cagatagatc gccggcacca atgccagcaa
    10561 ccagcctgtg aagaccaacc agcggtacct acgcaggctg cgactaaggc gcatcatgaa
    10621 ctgctggatt ttcttcactc cccggttctg acgcaatgcg tgcaggcgac attaccgcag
    10681 caccctgtgg atgatgtgcg cttatctggt tcctgagctg caaagacagc gatgttgcta
    10741 agacgctgcg cagtcgttgt gaaccggtga ccaaggggat ttcggtccca ctagccggcc
    10801 atggctttgt gggcttgggt ggcaaccggg tccgtgatgg cacgatgtcg gatggccgcc
    10861 gcggaccccg ggcggtgcga ggggaacccc gccgtcaaga gccttgtgtt gcagcccatt
    10921 tgcgagatgt ccggtgctcg gctaaccgtc gctgggtggg atctgctcgg gcggtggcac
    10981 gggaccctgc agcgcggtct cggtcgggtc ggcgaaatgg tcgccctgca gctgcacgcg
    11041 gggcagcggg ccggtgatcg ggtcgtcgtc ggagtcggtg gcgttctcgg tgcggaacat
    11101 gaagaagaac accacccagc cgatcgcggc gatcagtagc cagctgatca gccgatagag
    11161 cagcatcgcc gagatcgcat tgggcaatga cataccgctg gacaccaggc cgggcaccag
    11221 caccgcctcc acgatcaaca gcccgcccgg catcagcgga atcgtgccga ccgcgcgagc
    11281 ggccgcgtac gcaaccgtca gcccggcgac cgaggcgtgg tcgccggccg cgtacgccgc
    11341 gaagccgagg caggccacgt cggcgatcca gttgaacatc gaccagctga aggccacccc
    11401 caagtcacgc cggctcaggc tcaccgattc gagctgcatc agcgtctcgc gccatttgtc
    11461 caggccggtg tccgccggct tgccgcgcac cgagttgacc cacgccaaca cgcgggtgcc
    11521 gatgccttcg atcagctccg gccgcgacgc caccgcctga gccagcagca acaacgctac
    11581 gaaaccgcca agcgtgaaca gcaacgagaa cgggttgttc tttgcgccca ggaagaaggc
    11641 tccgcccaag ccgagcagcg ccagacccac cgcctgcagc acaccggaca tcaccaactg
    11701 ccacgacgcc acgacggtcg aggcgcccca catgcgttgc tgacggaaca ggaatgtcgt
    11761 cgacagcacc gggccgccgg gcagcgtggt gctcagcgag ttggcggcgt agaaggcggc
    11821 ttcggagcgc aattgcttga cgtgcacacc ggcggatctc agcagggtgc gctgaatctg
    11881 ggcgaagctg tgcatcgacg cggccgctgc caacaccgcc gcgagcaacc accaccagtt
    11941 ggcctcatac aggctcaccc aggctttggc cagctggtcc cagcccaacg cgatctcgac
    12001 ggccagcacg atcgcgacga cgccgagaat cacccatcgc acccaccagt acttgccgcg
    12061 cgggttctcc tcgcgggcgg gtgggccgac acccccagcg tgcggcgcac ccaccggcac
    12121 gcggggacct gccccggcgc gcgccagatg aagcttgcgg gtgagtgcgt cgtgcgacac
    12181 gtactttagg gtaaccgcgc aggtggcgcg gccaacgagg cgccgcatcg accgtgtcgt
    12241 cgtcgtaacc ggatcgtgcc ggccgggggc gccggggcgg ccctggggag gggctcgggc
    12301 cggtcttcgg acgacctctg ttggccttcg ccggtggacc gtgccagata gggtgacacc
    12361 atgtcagcaa acctcgatga caccgcggtc gagccgcttg tccgcaaggc cgcggcctgg
    12421 gcgtggcgtt tgctcatcat cctgaccgcg gcggtcgcgc tgctctgggt catagacaag
    12481 ctcgagatcg tcgtcgttcc ggtgttgctg gcgttgatca tcagtgcgtt gctggtgccg
    12541 gcggttgact ggatcgaccg gtggggcttg ccccgcggtg ccgcggtggc gctggtcttg
    12601 ctcgtcgggt tcgccatcct gggcggcatc cttgcattcg tcatcatcca gttcatcgac
    12661 ggtttgcccg acctgaccga acaggtgacc cgcagcatcg actccatccg caagtggctg
    12721 atcgaagggc cgttgcacgt gcgtggcgaa cagatcggca acgcgggcaa cgccgcgatc
    12781 caggcgctgc acaacaatca ggccaagctg accagtggcg cgctggccac cgcggcaacc
    12841 gtcaccgagc tggtgacagc cgcggtgttg gtgctgttca cgctcatttt cttcctctac
    12901 ggcggccgca atatctggca ctacgtcacc aaaatcttcc ccgtcagcgt ccgggagcgg
    12961 gtgcgcgagg cgggccgcgc cggttatcga tcgctgatcg cgtatatccg ggccaccttc
    13021 ctggtcgccc tgaccgatgc cgcgggtgtc ggtaccgggt tggcgatcat gggcattccg
    13081 cttgcgctgc ccctggcctc actggtgttt ctcggtgcct tcatcccgtt ggtgggtgcg
    13141 gtgatcgcgg gcttcctggc cgtgatcgtg gccctgctgg cgaagggatt cctctacgcg
    13201 ctgatcacgg tgggtttgct catcgcggtg aaccaactcg aggcacattt gctgcagccg
    13261 ttggtgatgg gccgcgcggt gtcgattcac ccgctcgcgg tggtgctggc catttccacc
    13321 ggcggtgtgc tcgccgggat cgtcggcgcg ttgctggccg ttccaacggt cgcattcctc
    13381 aacaatgcga tacaggtgct gctggctccc gatccgtccg ccgaggtcga aaagcagacc
    13441 gagggtgagg acgatttcgg ggtgatggtc caggccaagg cggacgaccc cagcgacgac
    13501 ccgaactgag cttgtcgggg ccttcggggc tacagccgtc cctcgcggcg cagcaggtcc
    13561 tgggcgctga ggccaccgcc gccgcgtcgg cgccggtcac cattgtcgct ttgtccgcgg
    13621 gcgttgagct tctcggtggc ggcgtcggag tcgttggcgt cgctacgcat gaccgggagg
    13681 gcggccgtcg ggtcggcagg gtcggccttg tcgtcgtcgg aacggctcgt cggcaccggc
    13741 atggcccggg tttgtccgct ggacggtgtg ggcggggagg gcggtgcctg cgcgcggggc
    13801 ggggcgggcg gcgtggagct gccgggacgg cggtcttcgg gtcgctgacg gccgggcgcg
    13861 gcctggccgc cgggaaccgc gttggtgttg gcgggaaccg agtggcgcgt cgtgctggcg
    13921 gtcggttggc tgggcccggt ctggatgcgc gcggtgctgg cgcccgacgg tgcgggcagc
    13981 tccgcagccg gcctgggctc gggcgggccc gagcgaaccg gccgcggcgg ctccagcgcg
    14041 ccagggtgcg tggggtcgtg cgcggggcgc gcggtggcgg cgaccaggct gggcgtcacg
    14101 accggaggcc gcgagggacg cccgttgagg gtgggccgct tgcgctcgtc gggcaggtcg
    14161 atctcgccga gcccgatgcg gttctgcagg cgccgcgccc agcgcggagc ccaccagcag
    14221 tcatcgccga gcagcttcat caccgagggc accaggaaca tccgcaccac cgtcgcgtcc
    14281 agcaacagcg ccgccatcag gccgaatgcc agatatttca tcatcaccag gtcggagaag
    14341 acgaatgaac cggcgaccac ggcaaggacc agcgcggccg ccgtgatgag tcggccggtg
    14401 gtcgcggttc cgatccggac ggcctcctgg gtcgacatgc cttcttcgcg cgcttcgacc
    14461 attcgggaca ccaggaacac ctcgtaatcg gtggccaagc catatcccac cgcgacgacc
    14521 agggcgatga tcaccaccat caagggtgtt ggcgtgaagt tcagccactt ggagaagtgc
    14581 ccgtcgacga agatccacgt caatatgccc atcgtggacc cgagcgtcag tgcgctcatc
    14641 accgcggcct tgacgggcaa cacgaacgac ccgaacgcca ggaacatcaa cagcatggtg
    14701 gtggtgatca aaacgagcac catcagtggg gccctcgcca ccaggctgtg aatcgaatcc
    14761 tgctcgaggg ccggggtgcc accgacccat agcgtgaggc ccttcggcgg cgtgatgctg
    14821 cgcaagtcgt tgattttctt ggccgcgtca ttcgggttga tcagcccgtt ctgcaggacc
    14881 cgcaccgcgg gatccttgga cgcatccggc gcgtagctgc gctcctgcca catgttggcg
    14941 gggttgttgt cctggtcgct gaacccgctg atcgccatcg ccttgctgcg gatgtcggcg
    15001 acctgctggt cggtgacggg ctggtggttg gtggactgga tcaccagtgt cagcggattg
    15061 gtgcggtacc cggggaaaag ttggtcgaaa tgctcctggg cctggcgcac cggattgttg
    15121 ggcggcaggt acttctcgct catgccgccg aaggacaggt tgcccagcgg gatcaccagc
    15181 aggatcatgc cgataacgat cgggatggcg aaggtgagcg gccgcttcat cacgaagttg
    15241 accagcttgc cccagaaccc ggcctcgacc tcttcgcggg tcttggtctt ctgcagccga
    15301 tccgcgagcc agttgaggta gccgcgcgac acccaccagt tgcgcaggaa cggcacccgg
    15361 aacagggtcc gcacaccgag cgcgtcgaca tttttaccca ggatccccag gcacgcgggc
    15421 agcagcgtga tcgacagcac ggccgcaagt gtcacggccg cgatcagggc ataggtcagc
    15481 gacttcacaa agccctgcgg caacagcagc aggctggccc cggacgcggc gatcagcacc
    15541 gccgagaagg tcaccgtgcg gcccgccgtc atgaccgtgc ggcgcaccgc ggtctcggtg
    15601 tcgtagccct cggcgatctc ctcccggaat cggctcacca cgaaaagccc gtagtccacg
    15661 gcgatgccca ggccgatcaa cgagaccacc ggttgggcga aaaagtgcac cggcccgaag
    15721 acggcgatca ggcgcaggat gcccagcgag cccgcgatgc tgagcccgcc gaccatcacc
    15781 ggcaggcacg ccgcgatcac tccgccgaac accaggaaca gcaccaccgc caccagcggc
    15841 agcgccagca cctccatgcg gcgctggtcg gtggcgatgg ttccggtcag ggcattggcg
    15901 accggctcga ggccggcgag ctggaccgtg ccaccgtcga gcttctgcaa gtcgggcgcg
    15961 atggtcttgt agttgttgag aatggagtcg tcgtcgttgc ccttgagtgg gatggtgacg
    16021 aatgtgtact tcttgtccgg ggtgaccatg ccctggatga ccgagctcgg gctgtccggc
    16081 gcccggaggt agccagccca gcccagcacc tggtcagggt ggtcctgctg gaacttgttg
    16141 atctcatcgg tgatctgctt ggcccaggcc gggtcggtga cgttcttgcc gtccggcgcc
    16201 ttgaagatcg cgacgatgtg cccggttcgg tcgcggccgt agaccttgtc gccgatgatc
    16261 gaggccttga cggactgact gccgtcgtcg taaaaaccgc tctgtgtgac atgctttccc
    16321 aggctcagcc cgaatgcgcc gccgataagg cacagagcta ccgtgacccc gatcacgatg
    16381 taccggtagc ggtacacagt tcgaccccac caggcgaaca cgtaagctcc ttactggatc
    16441 tgtagcgacc cgcgcatcgg ttttccagtg tcacacacgc gcggtcagca gcgcggacag
    16501 cggccggaac ggctgcagcc acgcgccctg gtcaggcagc gaatctaggc cgattcgcgg
    16561 caacggctcc cggaaaacgc cgtcgatgtc ttccagatcg acgaagtcca aggtatccga
    16621 cgctagtgcc caactcgcat gttcgcgaaa tccgatcacc tgaacaccga tgccgtcgcg
    16681 tgcgatttcc tccagcggct gacggaaagc ttgaccgtcg gcggacgcca cgatcagcgc
    16741 cgcgagacct tcggtgcgcc gcagctcgat gtgggcgagc atgtcgcggt cgacgtcgct
    16801 atcctcgtcg atcttcggct tggcgaagac cgcaaacccc acgttacgca acgcatccac
    16861 ccacggtcga accacatcgg cgctgcccgg agcgatattg gtgaaaacgg tggcctcggg
    16921 ttcgaccacg gcgcccggat gccccgcgct gacctccgcc gtgcgcgtca gcagccagcg
    16981 acccagtgcg tcgaaccggg ggcgttccat ggctgtcggg cgacggccca agatcgagcc
    17041 caggcccatg tcgaggttgg gcgcgtccca caccagcaga acgcgtccgc ccggagccgg
    17101 tgagaggctc gccagcgcgg caccggacgg cactcccggt gacgccaggg gttgcgatgc
    17161 cgctgcggtc tcctccaggc tcatagtccc catgtcattg ctttgctttc tctagagcct
    17221 tctccagacg aactcggtca cggagttccc cgcgtcgtgc gccttcgtct cgtacttggt
    17281 ggtcggccgg acgaccgaga tcggtagccg gctatccgcg tcggcccggc gcaaccgcgg
    17341 ttcgccatcg ccggcctcgg cgatgtgctc ggcgtacccg gggtggtccg tcgcggcgtg
    17401 cagcacaccg ccggggagta gccggtcggc gatcaggcca atcgtggccg gttgcaggaa
    17461 ccggcgtttg tggtggcgag ccttgggcca cggatcggga aagaacaccc gaaccccggt
    17521 cagcgagccc ggtgcgatcc gatgcgcgag cacgtcgatc gcgttgccgc ggatcaaccg
    17581 gatgttgcca acccgctccc ggtccatcgc gcacagcagc tgcgccagcc cgcggcggta
    17641 gacctccacc gcgatcacgt cgatgccggg ctcatccttg gccatcgcca atgtcgaggt
    17701 gccgctgccg cagccgatct cgagcaccac cggcgccaca cggccaaacc acacgccggt
    17761 gtccagcggc tccgcgccct cgcgcccccc tgaggcagtc cccgccgaga tgccgagttc
    17821 cggccacagc cgctcccagg tctcgcgctg ggcgccggaa agcgccgaac gccgcgagcg
    17881 gaaacccgtg gccgggagat aacccggttc ggggcggtcc ggatccggcg ccaggggcgc
    17941 ggtcatgccc tctccagtgg ctcgtccggc ttgccggccg aaccccacct ccaccggcgt
    18001 gtcgggacac gtccccaccc cgggttgcga atgcatttgt ccatggtggc ccatgaaccc
    18061 ccgatgtagc cgcccctggt ccagattgat acccaacagt tgccttcaac tggtaacaga
    18121 tagacagtgg ctcgcatctc ggtgacgcag gtgccaccat tcggtgggac tcgagcggtt
    18181 tgtcggcaaa cggacctggt cccggcgacg accccggatc cggtcggggc aacgccggcc
    18241 gggcaatcgg cccgggcgga tcgaacgggc aggacatgac gagacgagga caccagattt
    18301 tcgccgagga gctggccggc tttgctgccg gccacgccga cccgcgcgtg acggcgatcg
    18361 cggagcgggc agccgcgccg ctgcgggtgc tggtgtgcgg gcgcggcgga gtgggccgca
    18421 gcaccgtggc gcacgccctg gaccgtgccg gtggcgcatt ggggatctcg gcgacgaccg
    18481 gggcgacacc gccgacctgc gccggtgaca gcgcccccgc cttgggcgaa atattcgacg
    18541 aaataagcgc cgacgttgtt gtctacgtca ccgcggaggt ggtcaaaccc gaggacatcg
    18601 acgcgatcgg caccgccgga cccccggtgc tggcggtgct gaacaaagcc gacctggccg
    18661 gctctctttc cggacgggcg ggcgacgagc cgatggtggc ggcgcggatg cgctgcgcgg
    18721 acctgtccgc cctcgtcggg gtgccgatgg agccgatgat cggtctgctc gcggttgccg
    18781 cgctggacga cctcgatggc gcgctgtggg ctgcgctgcg ggtgctggcg gccgatccgg
    18841 atggtgcgac ctgcctcgac ggttcgttcg ccgggttcct ggacgccgac gttgcggtgc
    18901 ccaccgacgt gcggctgcgc ttgctggaca cgctcgacct gctcggcacc gcgctcggca
    18961 tcgccgccgt ccaacagggc aggacgccgg cgcagttgcg ggcgttgctg cgccggatga
    19021 gtggcgtcga cgccgtcctg accgcggtcg ctgccctcgg tgccgaggtg cgttaccagc
    19081 ggatgctcca ggtcgtcgcg gagttggagg ccctggccgt cggtcaggga gatctcgggg
    19141 aaagcatcag cgggttcctg tcctgcgacg acacggtggt cgcccgcatg gcggtcgccg
    19201 ttgacctggc cgaggcggcg gggctgggcc ccatcgatct ggcccccggc aatctggatc
    19261 gcgacgaccc cgccgcgcac ctgccacggg cggtgcattg gcagcgctac agccttggac
    19321 gtttgggccc ggtgagcgat ctgcatcggc tctgcggcac cgatatcgcc aggggatcgc
    19381 tgcggctgtg gtcgcagacg tgcggctcgc tgcccggaga atcctggtga cacccaaggc
    19441 cgccggtgac cccgacaccc aggtcgacgc tctcgtggca gcgatcgggc cccggctgga
    19501 ggtgcccgcc atcaaccgcc gcgacgtggt gttggtgacc ggcccgtgga tggccggggt
    19561 cagctcggtg gtcggggtgc tgcgcgaacg gctgccgcgg cacactttcg tcgagtccac
    19621 ggatctggca accggtgatg cgccgatcgc ggcggtcttc gtcgtatccg cggcggccca
    19681 gctgaccgga tccgattgtg cacaactgga tgccgccgcc gagaacaccg atgtggtcgt
    19741 cggtgtggtg tccaagatcg acgtgcaccg caactggcgt gacgtggttg ccgccgacca
    19801 cgacatcctc gcggaccacg caccgcgcta tgccaaggtg ccctgggtcg gtgtggccgc
    19861 cgcgcccgac ctgggcgaac cgatcgtcga cgagttggtc gagaccgtcg cggcgcgcct
    19921 ggccgagccc gacattgcac gacgaaaccg gttgcgcgcc tgggattccc ggctgcagac
    19981 ggtcgccaga aggttcgatc gcgacgcgga gggctccggt cgacgggcac gcgttgacac
    20041 gttgcgcgaa gagcgcagca cggcattgcg gcagcggcgc cagtcgaaga ccgagcgcac
    20101 cgtcacgctg cgcggccaga tccagcaggc cagggtccag ttgtcctact ttgcgcgcaa
    20161 ccgttgctcg tcggtgcgta ccgaactgca ggaggacgcc gcggatttgt cgcggcggaa
    20221 catgtcccgc ttcgaggcgc acacccgcgg ccgcgtcgac gaggtggtcg cggaggtgag
    20281 cgagggcacc agcacccagc tcgtcgacgt cgcgcacgtg atgggtgtgc cgatggccct
    20341 gccagccctc gagaagctgc cgggcgtcga ggtctcgccc ccgcccctga aatcgcggcg
    20401 ccacgaaacc tggctgatga tgctgctggg cgccgggttc ggattggggg tggcgctcac
    20461 gctgggtcgg ctggtgggcg ggctggcctc ccggcttagc cccggccttg ccatcgccgg
    20521 cgtagcgggg tgcgtggtga tcggcctggc ggtcacgttc gtggtgatcc acatccgcgg
    20581 cctgctgcgc gaccgtgcgt tgctggaccg ctgggcgggc gacgtcacgt cgtcgctgcg
    20641 ttctgtggtg gaggagctgg tcgccacccg cgtgctggtc gccgagtcgg tgctgagcag
    20701 cgcgctgatc gcccgcgacg aggccgagaa cacgcaggtg gccgaccagg tcagcgccat
    20761 cgacagcgaa ctgcgcgagc acgcgatcgc cgcggcgcgg gccgcggcgg tgcgtgaccg
    20821 ggaaatgccg gcggttcagg ccgcgctgga ggctgtgcgt gccgaactgg gagaatcggg
    20881 cgtatcacaa tccggaaatt ccgcctcgga tcgcagcgag gaaaaaaacg acaaaccggg
    20941 tctcgacgag gacgaatgac gccgttttcc ggcgtttggc ggagcttctg aatcgttgat
    21001 gtgagaaggc ttatacccgc ccgaggcttc cccgacaagt ggcgcgggat aacctgtagt
    21061 taacgccacc atgtaaatag ttgtgtgggc gaatgcatac gtaacgacga gagccgtaag
    21121 caggcagaag aacccaggag agttcgatga cttcagcgac cattcccggt ctggataccg
    21181 cgccgaccaa tcacccgggg ctgctgtcct gggtagagga ggtcgccgaa ctcactcagc
    21241 cggaccgggt ggtcttcgcc gacggctccg acgaagagtg tgagcggctc tccgcccagt
    21301 tggtcgaagc cggcacgttc acgaagctga acgagaagaa gcaccccaac tcctacctgg
    21361 cgttgtcaga tccatccgac gtggcgcggg tggagtctcg gaccttcatc tgttctcgcc
    21421 gggagatcga cgcaggcccg accaacaact ggatggaccc cgccgaaatg cgctccatca
    21481 tgaccgacct gtaccgcggc tgcatgcgcg gtcgcaccat gtatgtggtg ccgttctgca
    21541 tgggcccgct cggcgccgac gaccccaagc tgggcgtgga gatcaccgac tccgagtacg
    21601 tcgtcgtctc gatgcgcacc atgacccgga tgggcaaggc cgcgctggag aagatgggtg
    21661 acgacggctt cttcgtcaag gcgctgcact cggtcggcgc cccgctggag ccgggtcaag
    21721 aggacgtgcc ctggccctgc aacgacacca aatacatcac ccacttcccg gagacccgcg
    21781 aaatctggag ctacggctcc gggtacggcg gcaacgcgct gttgggcaag aagtgctact
    21841 cgctgcgcat cgcctcggcg atggcccacg acgagggctg gctggccgag cacatgctga
    21901 tcctcaagct gatctccccg gagaacaagg cgtactactt cgccgcggcg ttcccgtcgg
    21961 cctgcggcaa gaccaacctg gcgatgttgc agccgaccat cccgggctgg cgcgccgaaa
    22021 cgctcggtga tgacatcgcc tggatgcgat tcggaaagga cgggcggctc tacgcggtca
    22081 accccgagtt cggattcttc ggggtcgcgc cgggcaccaa ctggaagtcc aaccccaacg
    22141 cgatgcgcac catcgccgcg ggcaacacgg tgttcaccaa tgtcgcgctc accgacgaca
    22201 acgacgtgtg gtgggagggt ctggagggcg acccgcagca cctcatcgac tggaagggca
    22261 acgactggta cttccgcgag acggaaacga atgcggccca tcccaattcg cgttactgca
    22321 ccccgatgtc gcagtgcccg atcctggcgc ccgagtggga tgacccgcag ggggtgccga
    22381 tctcggggat cctgttcggt ggccgtcgca agaccacggt gccgttggtg acgcaggcgc
    22441 gcgactggca gcacggtgtg ttcatcgggg ccaccctggg ttccgagcag accgccgccg
    22501 ccgagggcaa ggtcggcacc gtgcggcgcg acccgatggc catgctgccg ttcctgggct
    22561 acaacgtcgg cgactacttc cagcactgga tcgatctggg caagaactcg gacgagtcga
    22621 aactgccgaa ggtgttcttc gtcaactggt tccgccgcgg cgacgacggt cgcttcctgt
    22681 ggccgggatt cggtgagaac agccgggtgc tgaagtggat cgtcgaccgc atcgagcaca
    22741 aggccggcgg ccaggacacc ccgatcggca tcgtcccgac cgccggggac ctcgacttgg
    22801 acgggttgga cgtcgacggc gccgacgtcg aaaaggcttt ggtggtcaat gccgacgaat
    22861 ggcgcaagga actgccgttg atcgaggagt ggttcgagtt cgtcggcgac aagctgccca
    22921 ccggcgtcaa ggacgaattc gaggcgctca agcagcggct gtccgacgcc gactaggtcg
    22981 agctgggcac gcggggctag ccggcccccg gagatcggct gatcagttgc cgtcgcagtg
    23041 acttcggcag gtacaccgcg cccgatccgg cgactgctgc cgagtcgccc gggttggaga
    23101 tggcgcagcg cttcagtgac aggcagccgc acccgatgca cgagtccagg ttgtcgcgca
    23161 gcgcgattag cccggcgatc tggtcatcga ggcgtgtgcg ccagcttttg gacagcccgg
    23221 cccagtcggc gcgggtcggg gtgcggcttt gcggaagctt tgccagcgca tcggcgacct
    23281 catccaggct caacccgacg tttcgggcgg cccggatgaa cgccaggcgc cgcagtgtct
    23341 gccgttcgaa ccggcgctga cccccagcag tccgggtcgc cgagatcagg ccctggctct
    23401 cgtagaaccg gattgccgac gctgcgaagc cgctgcgatg ggctatctca ctgacggtga
    23461 acaaatcccg cttatccatg tcgcctactc gaattgaaga cttgacttaa agtcaacttt
    23521 aactaccagt atggccttat gactcgatct caacctcatc ccgtcgcaat tgtcaccggc
    23581 gcgtcgcgtg gcttcggcaa agcggtgacc gccgcgttgc tcgagcgcgg ctgggtcgtc
    23641 gtcggcgacg cccgccgcgc tgtcgaactg aaaaccactg ctgcagagct gaattcaacg
    23701 agattgatcg ccttgcccgg cgatatcacc gacgcgtcgc atcgtgccgc actcgtggcc
    23761 gccgcactgc gggccgggcc gctccgcctg ctggtgaaca acgccagccg actaggaccc
    23821 agcccgcaac cggcgctcgc cgactacccg ccgaaccagt tgtgggccgt atatcagacc
    23881 aacgtgctcg ccccgctcgc gctgatccag gcgacgctgc cggcgctggc cgacaacgcc
    23941 ggcgtgatca tcaacgtgac ctccgacgcc gccgtcgagc cgtatccggg ttggggcgga
    24001 tacggctcgt cgaaggccgc gttggatcag ctgtcggccg tgctggccgc tgaggtctca
    24061 gtaacctcag cggccccggc ggtggctgtt tacgccttcg acccgggcga catgcggacc
    24121 gagatgcatc aggcggcgtt ccccggcgag gacatctccg accgcgccga gccggaggcg
    24181 aaagtccccg cgttgctgcg cctgctggac accaggccgg ccgccgggcg ttaccgggcg
    24241 gcggacctga caacggcggg agcgcggtga tgactctcgc gatgagcccg caaacgcact
    24301 tccaccggcc gcccggctcc gacgccttcg agccgccgga agctcacggc gtcgcccggg
    24361 acgcggtcaa gttgctggtc gcccggccgg gcggggtcac tcacacccga ttcgccgacc
    24421 tcggtgctca gctgcgaccg ggtgatgtcc tggtggtgaa caactccgcc accctgcccg
    24481 ccgccgtcga cgggcgccgt gccgggcggc tcatcgccgt gcacttctcc atggcgcgcg
    24541 ccgaaaaact atgggtggtg gaaccgcggc tcgcggacac ggccgagcac ctcacggaca
    24601 tccagccggg cgaacgcatc gacctgcccg gcggcgcagc gctggtcatc gggtcgtcgt
    24661 atccggaacc tgggctcgga ggcgcacggt tgtggaccgc gcgggtgatc ctggagggtc
    24721 cggcaggcgc cgtcacctcc tacctcggcc ggtatggccg gccgatcagg tattcgtacg
    24781 tgccgcgcgc atggccgttg cgctactacc agaccgtctt cgccaggagc gccggaagcg
    24841 ccgaaatgcc cagtgcggcg cgccctttca ccactcagct ggtaactgct ctggtggccg
    24901 ccggcattgc gatcgcgccg atcacgctgc acgccggggt gtcgtcggcg gaggcgggcg
    24961 agccaccgac tcccgagccg ttcgcggtgc cggcggccac cgctcggatg gtcaactcgg
    25021 cgcacgcggc cgggagccgg gtgatcgcgg tcggcaccac ggcgacccgt gctctcgagt
    25081 cggccgcgaa cgccgatggc gtggtacgtc cggccgccgg ctggacggat ctggtgctgg
    25141 gtcccgggcg cccgtgccgg gtggtcgacg gcctgatcac cggatggcat gacgccggcg
    25201 cctcgcacct gttgctgctc gaggccgccg cgggccccga gctggtcgcc atggcctatc
    25261 gggaggccgt cgagcacggc tatctgtggc atgagttcgg cgacagcgcc ctgttgctgc
    25321 ccggccgcgg gtatcgcccg ccctcgacga ccggccgaca agcgcccgct tgacacccgt
    25381 caaggcgcgg gtcgtacagt cggcccatgc agattcgccc gtacacatcc ggccgggggg
    25441 gtgccaacaa gcccgccgtg atcctgcacc cgtccgggac ggtcatcacc ttcgacgacc
    25501 tggaggcgcg cgctaaccgg ctggcgcatc ggttccgcca ggcgggtctg cgtgagggcg
    25561 acaccgtcgc aatcctgatg gagaacaacg agcacatcca cgcggtaatg tgggcggccc
    25621 ggcgcagcgg tctgtactac gtgccgatca acacgcacct gaccgcggcg gaggccgcct
    25681 acatcgtcga caacagcggc gcgaaggcca tcatcgcctc ggcggcgttg ctcgacacct
    25741 gcgcgggtct cgccgactac ctgccgaatg ggctgccgga cttgctgatg ctcgcctccg
    25801 gcaccgaaga cgccgacctg ccgggctggg agcgctaccc ggaatgcgtt gcaggtcaac
    25861 cggacacccc gatcgacgac gaacgcgagg gcgacctgct gcagtactcg tcgggcacca
    25921 ccggccggcc caaggggatc aaacgccaac tgccgcatac ctcgcccgcc gagtcgccgg
    25981 gcatgatgtc ggcgctggtc aatttttgga tgacccccga gtcgatctat ctgagcccgg
    26041 ctccgctcta ccacaccgcg ccgtcggtgt ggtcgatgag cgcgcaggcc ggcggcgtca
    26101 cgaccgtcgt catggagaag tttgaccccg agggcacgct ggacgccatt gggcgctacc
    26161 aagtcaccca cggccagttt gtgccggcca tgttcacccg gatgctgaaa cttcctgagg
    26221 ccgtgcgcga ttcatacgac gtgtccagcc tgcagcgggt gatgcacgcc gcagcgccgt
    26281 gccctgtgca gatcaagaag gagatgatcg actggtgggg gccgatcgtc gacgaatatt
    26341 acgcctcctc cgaggcgata ggctcgacgc tgatcagcgc cgaggaatgg ctggcgcatc
    26401 cgggttcggt cggcaagccc atgatgtgcc agatccacat cgtcggcgag gacggcaacg
    26461 agctgcctcc ggggcagccc ggcgagatct atttcgaagg cgggtattcg ttcgagtacc
    26521 tcaacgatcc gtcgaaaacc gctgcgtcgc gcgagaagca cggctggacc actgtcggcg
    26581 acgtcggcta cctcgacgac gagggctatc tctacctgac cgaccggcgc caccacatga
    26641 tcatctccgg cggggtgaac atctacccgc aggaagccga gaatctgctc gtcacacacc
    26701 cgaaggtgtt ggacgccgcg gtgtttggcg ttcccgacga cgagatgggt cagcgtgtgg
    26761 tggccgccgt gcagaccgtt gaccctgccg acgccaccga tcagttcgcc gacgagctga
    26821 ttggctggct gcgagaccgg ttgtcgcact tcaagtgtcc gcgttcgatc gcgttcgaga
    26881 cgcagttgcc gcgcaccgac accggcaagc tctacaagaa cgcgctgatc gagaagtatt
    26941 cggtgtgata ccgatgctcc gcgtggtcga cctgtcgagc gcgccggacg ccgacgtccc
    27001 agtgccgccg ggggt
//
LOCUS       DASSGQ010000198         9091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_74500, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000198 DASSGQ010000000
VERSION     DASSGQ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9091
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74500"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VFI55_08765"
     CDS             complement(<1..581)
                     /locus_tag="VFI55_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7074155.1"
                     /translation="MSQTVRGVISRRKGELVELVDIVVPDPGPGEAVVDVIACGVCHT
                     DLTYRDGGINDDYPFLLGHEAAGTVEAVGPGVTAVEPGDFVILNWRAVCGQCRACKRG
                     RPAYCFDTFNAEQKMTLTDGTELAPALGIGAFADKTLVHSGQCTKVDPQADPAVAGLL
                     GCGLMAGLGAAINTGAVDRNDTVAVIGCGGVGDA"
     gene            683..1702
                     /locus_tag="VFI55_08770"
     CDS             683..1702
                     /locus_tag="VFI55_08770"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7074156.1"
                     /translation="MVAAIDGASLALLLSIGHQTGLLDTMAGLGPATSAQIAEAAGLN
                     ERYVREWLAGVTTGRVVDYDPLTATYLLPAHRAGVLTRAAGPDNLALVSQIIPLLSEV
                     EQKVIGCFREGGGLPYSEFPRFHTMMAEMSGVVFDSALVDVVLPLVDGLVERLRTGAD
                     VADFGCGSGHAINLMARAFPASRFTGIDFSDEAIAAGIREAGRTGLTNVTFESHNLAD
                     LDKAEAYDVITVFDAIHDQGQPARVLENIHRALRPGGVLLMADIKASSRLEDNIGVPM
                     STYLYATSLMHCMTVSLAVGGAGLGAAWGTQLATSMLADAGFDNVRVAEVDSDPINSY
                     YIAGK"
     gene            1702..2532
                     /locus_tag="VFI55_08775"
     CDS             1702..2532
                     /locus_tag="VFI55_08775"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7074157.1"
                     /translation="MAASDALDAIDDWPVTAVAAAVVGPGGVLDSHGDTERVFELASV
                     TKPLVARALHVAIEEGVVELDTAAGPPGATVRHLLAHASGLAMHSDRVLAKPGTRRVY
                     SNYGFTVLAETVERESGIEFGSYLAEAVCQPLGMAATRLNGGNAAAGFGATSTVSDLA
                     VFAGELLRPTTVSAQLHAEATTVQFPGLDGVLPGYGAQRPNDWGLGFEIRDSKSPHWT
                     GARNSARTYGHFGQAGGFIWADPELALALVVLTDRDFGEWALKPWPAISDAVIREHAS
                     "
     gene            2681..3193
                     /locus_tag="VFI55_08780"
     CDS             2681..3193
                     /locus_tag="VFI55_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3145 domain-containing protein"
                     /protein_id="HET7074158.1"
                     /translation="MRASNQFADVTTGVVYVHASPAAVCPHVEWALSSSLQSKANLVW
                     TPQPAMPGQLRAVTNWVGPVGTGGRLANALRSWSVLRFEVTEDPSPGIDGHRFSHTPQ
                     LGLWSGSMSANGDVMVGEMRLRTMMAQGADTLAAELDSVLGTAWDEALEVYRDGGDAG
                     EVTWLSRGVG"
     gene            complement(3282..4199)
                     /locus_tag="VFI55_08785"
     CDS             complement(3282..4199)
                     /locus_tag="VFI55_08785"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HET7074159.1"
                     /translation="MTRLRREISKVTALTNPVSGHGAALRAAQIAIARLQHRGVEVVE
                     IIGDNAEDARFLAGAALERGTDALMVTGGDGVVSNALQVLAGTDVPLGIVPAGTGNDH
                     AREFGIPTKDPEAAADIIIDGWMETIDLGRIRDAKGVNKWFGTVAATGFDSLVTDRAN
                     RMSWPHGRMRYYIAMLAELSQLRTLPFRLVLDGTRKIDADITLAAFGNTRSYGGGLLI
                     CPDADPTDGLLDITMAHSASRTKLVRLFPTVMKGTHVDLDEVSTARAKSIHVECPGIN
                     VYADGDFACPLPAEISAVPAALQVLRPAR"
     gene            complement(4196..5776)
                     /locus_tag="VFI55_08790"
     CDS             complement(4196..5776)
                     /locus_tag="VFI55_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015575176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7074160.1"
                     /translation="MKWNAWGDPAAAKPLSEGIRSLLKHAVGLQDSDKAQLDADQVRL
                     RPSALAPADRAALAAIVGAEYCRTDDPDRLLHAGGKSTIDLLRRKDSGVQDAPDAILL
                     PGTEDDVAAVLRYCSEHEIAVIPFGGGTNVVGGLNPSRGEFAAVVSLDLRRFDRLISL
                     DEVSGQAVFGAGVTGPDAERLLGERGFSLGHFPQSFEYATIGGFAATRSSGQDSAGYG
                     RFNDMVRGLRMITPVGTLDVGRGPESAAGPDLRQLVIGSEGTFGVITQVRLRVHRAPE
                     AVRYEAWSFPDFGTGAAALRAVTQNATGPTVIRLSDEAETGVNLATTESIGENQVTGG
                     CLGITVFEGTKEHVESRHAETSALLAAHGGTSLGEAPARAWERGRFGAPYLRDSLLSA
                     GALCETLETATDWSNVPALKAAVTEALTNALAESGTPALVMCHVSHVYPTGASLYFTV
                     VAGQRGNPIDQWRAAKKAASDAIVATGGTITHHHAVGADHRPWMRDEVGELGVQVLRA
                     VKATLDPAGILNPGKLIP"
     gene            5824..6393
                     /locus_tag="VFI55_08795"
     CDS             5824..6393
                     /locus_tag="VFI55_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7074161.1"
                     /translation="MLSISNVPSDTGDRILAAAASCVVDFGVDRVTLAEIARRAGVSR
                     PTVYRRWPDTKSIVAALLTQRITGVMRDAPLLGDDRESLVRQIVTVADLLRRDELVMS
                     VLHSQLAPIYITERLGASQQMLIDALAERLRVAQRHGSVRAGDPLQMATVVLLITQST
                     IQSEQIVRPILDADALATELAYSLNGYLA"
     gene            6381..7928
                     /locus_tag="VFI55_08800"
     CDS             6381..7928
                     /locus_tag="VFI55_08800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HET7074162.1"
                     /translation="MPSVNTAALNAARRAADLTALADGQPLDVLVIGGGITGAGIALD
                     AASRGLRVALVEKHDLAFGTSRWSSKLVHGGLRYLATGDVGIARRSAIERGILMTRNA
                     PHLVHAMPQLVPLLPSMSHGKRALVRAGFLAGDALRMLAGTPSSTLPRSRRVSARHVV
                     EMAPTVLRDGLDGGLLAYDGQLIDDARLVTAVARTAAQHGARILTYLAASEASGTSAR
                     LTDQRTGESFDVSAGAVINAAGVWAGEIDGSLRLRPSRGTHLVFAAAAFGNPTAALTI
                     PIPGELNRFVFAMPEQLGRVYLGLTDEAAPGPIPDVPEPSTDEITFLLDTVNTALGTA
                     LATTDVIGAYAGLRPLIDAGGAGFDGRTADVSREHAVVESASGVISVIGGKLTEYRYM
                     AQDVLDRAISLRHLHAGDCRTRNLPLIGAPANPAPFAARAAGLPASLVARYGAEASNV
                     IATATCDRPGDPVAEGIDVTRGEFEYAVTHECALQVDDILDRRTRIGLVARDRERVVA
                     AAEEFLA"
     gene            complement(7937..>9091)
                     /locus_tag="VFI55_08805"
     CDS             complement(7937..>9091)
                     /locus_tag="VFI55_08805"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7074163.1"
                     /translation="SSRVEEVTKMASAKSVSRVVALEEAFVHQRVWDLFPPPLQRRYQ
                     PVRARLSDVGAGRIRLMDAGGIDVQVLSLVQPGVQILADDEAELAVAVSREVNDWLAD
                     AIAAYPARFAGFATLPTQSPPHAADELQRTVRDLGFVGALINGHTNGRYLDDPTFDPL
                     LARAASLGVPIYIHPTDPPAAVSDVYYAPFGSALVPTWGWPVETGTHLLRLICAGVFD
                     RHPDLKIIVGHMGELLPYCFTRLNAGLTMAGWLLAAESGNQDEPHTVMRNTVGYYLRK
                     NVFITSSGVFDVPVFDCARAMLGLDNLMFSVDYPFQDNFAATEFLQRCDLSPDDRERF
                     AHHTAEVLLGLGNRPQSVRPAGAVLGDAWYRLRSRVQSKVGRALISALVR"
BASE COUNT         1486 a         3015 c         3108 g         1482 t
ORIGIN      
        1 gcgtcgccca cgcctccgca gccgatcacc gcgacggtgt cgttgcggtc gaccgcgccg
       61 gtgttgatcg ccgcccccag cccggccatc aacccgcaac ccagcaggcc cgcgacggcc
      121 gggtcggcct gaggatcgac cttggtgcat tgacccgagt gcaccagcgt cttgtctgcg
      181 aaggcgccga tgcccagcgc cggagccagt tcggtgccgt cggtcagcgt cattttctgc
      241 tcggcgttga acgtgtcgaa gcagtacgcg ggcctgccgc gtttgcacgc ccggcactgc
      301 ccgcagacgg cgcgccagtt caggatcacg aagtcgcccg gctcgaccgc ggtcacaccc
      361 ggcccgaccg cttcgaccgt gccggcggcc tcgtggccga gcaggaaggg gtagtcgtcg
      421 ttgatgccgc cgtcgcggta ggtcaggtcc gtatggcaca ccccgcaggc gatgacgtcg
      481 acgacggcct ccccgggccc cgggtccggg acgacgatgt ccaccaattc gacgagttcg
      541 cccttccttc gcgaaatcac gccgcgcact gtctgactca tggcttccaa cctacggtgt
      601 agcgtcgctg agcgtgacgg cactcgacaa aggcgccacc ggcgagactg cctccgaaac
      661 cgccgaggca ttcaccgaac gtatggtcgc ggccatcgac ggtgccagcc tggcgctgct
      721 gctcagcatt ggacatcaga ccggtttgct cgacacgatg gcgggcctgg ggcccgccac
      781 cagtgcgcag atcgccgagg cggccggtct caacgagcgc tacgtgcggg agtggctggc
      841 cggcgtgacc accggacgcg tcgtcgacta cgacccgctg accgccacct acctgttgcc
      901 cgcccaccgc gcgggcgtgc tgacccgggc ggccggcccg gacaatctgg cgttggtgtc
      961 gcagatcatt ccgctgctca gcgaggtcga gcagaaggtg atcggctgtt ttcgtgaggg
     1021 cggtgggctc ccgtacagcg aatttccccg cttccacacg atgatggccg agatgagcgg
     1081 tgtcgtgttc gacagcgccc tggtcgacgt ggtgctgccg ttggtcgacg gcctggtcga
     1141 gcgactgcgt accggcgccg acgtggccga tttcggttgc ggcagcggtc atgccatcaa
     1201 cctgatggcc cgtgcgttcc cggccagccg gttcaccggc atcgattttt ccgacgaggc
     1261 cattgcggcc ggcatccggg aagcgggccg caccggcctg acgaacgtga ccttcgaaag
     1321 ccacaatctg gccgatctcg acaaggccga ggcgtacgac gtcatcacgg tgttcgacgc
     1381 catccacgac caggggcagc cggcccgggt gttggaaaac atccatcgtg cgctgcggcc
     1441 cggcggtgtg ctgctgatgg ccgacatcaa ggcgtcgagc cggctcgagg acaacatcgg
     1501 cgtgccgatg agcacctatc tgtacgcgac gtcgttgatg cactgcatga cggtgtcgct
     1561 ggccgtcggt ggcgccggcc tgggcgcggc ctggggaacg cagctggcga cgtcgatgct
     1621 cgccgacgcc gggttcgaca acgtgcgggt cgccgaggtc gactccgacc cgatcaacag
     1681 ctactacatc gccgggaagt gatggccgct tccgacgctc tggacgctat agacgactgg
     1741 ccggttacgg ccgtcgccgc cgcagtggtt gggccaggcg gagtgctcga cagccacggc
     1801 gacaccgagc gggtgttcga actggcgtcg gtcaccaagc cgctggtggc ccgcgccctg
     1861 cacgtcgcca tcgaagaagg ggttgtcgag ctcgataccg cggcgggccc acccggcgcc
     1921 acggtccgcc acctgcttgc gcatgcctcg ggcctggcga tgcactccga tcgcgtgctg
     1981 gccaagccgg ggacccgccg ggtgtattcc aactacggct tcaccgtgtt ggccgagacc
     2041 gtcgagcggg agtcggggat cgaattcggc agttacctcg ccgaggcggt gtgccaacca
     2101 ctgggcatgg cggcgacgcg gctgaacggc ggcaacgcgg ccgccgggtt cggagcgacc
     2161 tcgacggttt ccgatctggc ggtcttcgcc ggtgaattgc tgcgcccgac gacggtctcg
     2221 gcgcagttgc acgccgaggc gacgacggtg cagtttcccg gcctggacgg tgtactgccc
     2281 gggtacggcg cgcagcggcc caacgactgg gggctgggct tcgagatcag ggattcaaaa
     2341 tcgccgcact ggacgggtgc acggaactcg gcgcgaacat acgggcattt cggccaagcg
     2401 ggtggcttta tctgggcaga tcccgagctg gcgctggcgc tggtggtcct caccgaccgc
     2461 gatttcggcg agtgggccct gaagccgtgg ccggcgattt ccgacgcggt gatacgcgag
     2521 cacgcctcgt gaaaaatgca cactagcgca acaggggcat cacaaggcac aatagacacg
     2581 caagacacaa aatatccggg gaatatcggc aagctgccct gcttatgggt ccgccaggtc
     2641 gttggggaag acgtccttcg tggaaccaaa ggagcaggag atgcgtgcgt cgaatcaatt
     2701 cgccgacgtg acgaccggtg tggtgtacgt ccacgcctcg cccgcggcgg tgtgcccgca
     2761 tgttgagtgg gcgttgtcgt cgagcctgca gtccaaggcc aacttggtat ggacaccgca
     2821 gccggcgatg cctgggcagc tgcgcgcggt caccaactgg gtcggtccgg tgggcaccgg
     2881 cggccggttg gccaacgcgc tgcgctcgtg gtcggtgctg cgcttcgagg tcaccgagga
     2941 ccccagtccg ggaatcgacg gccaccggtt cagccacacc ccgcaattgg gtctgtggag
     3001 cggatcgatg agcgccaacg gggacgtcat ggtcggggag atgcggctgc gcacgatgat
     3061 ggcccagggt gccgacacgc tggccgccga actggattcg gtgctgggaa ccgcgtggga
     3121 cgaggcgctc gaggtgtacc gcgacggcgg tgacgcgggt gaagtgacct ggctcagccg
     3181 gggcgtcggc taacggtccc gggagcgata ggcgatcgtc aaaagcgata gcgcatgtgc
     3241 tagcgcattc gaagatcggc taatggtcgg aggccggatg ctcagcgtgc cggccgaaga
     3301 acctgcaggg ctgcgggaac cgcggatatc tcggcgggca gcgggcaggc gaagtcgccg
     3361 tcggcgtaca cattgattcc gggacactcg acgtgaatcg acttggcgcg tgccgtgctc
     3421 acctcatcga ggtcgacgtg ggtgcctttc atcacggtgg ggaacaggcg gaccaacttg
     3481 gtccgtgacg ccgaatgcgc catggtgatg tcgaggagac catcggtggg atcggcgtcc
     3541 ggacagatca acagcccgcc accatagctg cgggtattgc cgaaggcggc aagggtgata
     3601 tcggcgtcga tcttccgggt tccgtcgagt accaaccgga acggcaacgt tcgcagctgc
     3661 gaaagctcgg cgagcatcgc gatgtagtag cgcatccgcc cgtggggcca gctcatccgg
     3721 ttcgcgcgat cggtgaccag cgagtcgaac ccggtggccg ccacggtgcc gaaccacttg
     3781 ttgacacctt tggcgtcgcg aatccggccc aggtcaatgg tttccatcca gccgtcgatg
     3841 atgatgtccg cggcggcctc gggatccttt gtgggtatgc cgaattcacg agcgtgatcg
     3901 ttaccggtgc cggccgggac gattcccagc gggacgtccg tgccggccaa cacctgtagc
     3961 gcgttggaga cgacgccgtc acccccggtg accatgagtg cgtcggtgcc cctttccagg
     4021 gcggcgccgg cgagaaaacg cgcatcctcg gcattgtcac cgatgatctc gacgacttcc
     4081 acgccccggt gttgcaaccg ggcgatcgcg atttgcgccg cgcggagcgc cgcaccgtgc
     4141 cccgaaacgg gattcgtcag tgccgtgacc ttgctgattt cacgccggag cctggtcacg
     4201 gaatcagctt gccggggttg agaattccgg ccggatccag cgtcgccttg accgcgcgca
     4261 acacttgcac gcccagctcg ccgacctcgt cgcgcatcca gggccggtgg tcggcgccga
     4321 ccgcgtggtg atgggtgatg gtgccgccgg tggccacgat cgcgtccgac gcagccttct
     4381 tggctgcccg ccactggtcg atcgggtttc cccgctgccc cgcgacgacg gtgaagtaca
     4441 acgacgcacc agtggggtac acgtgcgaga cgtggcacat gaccagcgcc ggagtgcccg
     4501 attcggccag tgcgttcgtc agcgcttcgg tgacagcggc cttgagcgcg ggaacgttag
     4561 accaatcggt ggcggtctcg agggtttcgc agagtgcgcc cgccgacagc agcgagtcgc
     4621 gcagatacgg cgcgccgaac cggccccgct cccacgctcg ggccggcgct tcgcccagcg
     4681 atgtgccgcc atgggccgcc agcagtgcgc tggtctcggc gtgccggctc tcgacgtgtt
     4741 ccttggtgcc ctcgaacacg gtgattccca ggcacccgcc ggtgacctgg ttttcgccga
     4801 tcgactcggt agtggcgagg ttgactcccg tctcggcctc gtcggagagc cgaatgacgg
     4861 tggggccggt cgcattttgt gtgacggcac gcagggccgc agcccccgtc ccgaaatcgg
     4921 gaaacgacca cgcctcgtag cggacggctt cgggggcccg gtgcacgcgc aaccggacct
     4981 gcgtgatgac cccgaaggtg ccctccgacc cgatcaccag ctgccgcagg tcgggacccg
     5041 cggccgactc gggtccccgg cccacatcca gcgtgcccac gggcgtgatc atccgcagtc
     5101 cgcggaccat gtcgttgaac cgcccgtagc cggcagagtc ctggccggac gaccgtgtcg
     5161 ccgcgaagcc gccgatcgtg gcgtactcga agctctgcgg gaaatgcccg agcgagaagc
     5221 cgcgttcgcc gagcaaacgt tcggcgtcgg gaccggtgac gccggcgccg aaaaccgcct
     5281 ggccggacac ctcgtccagg gagatgagcc gatcgaagcg gcgcaaatcg agtgagacca
     5341 cagcggcgaa ctcaccccgg ctcggattga gcccacccac gacgttggtg ccgccgccga
     5401 acgggatcac ggcgatctcg tgctcggaac agtaacgcag taccgcggca acgtcgtcct
     5461 cagtgccggg cagcaggatg gcgtcgggcg cgtcctgcac tccggagtcc ttgcgccgca
     5521 gcaggtcgat ggtggacttg ccgccggcgt gcagcaatcg atcgggatcg tcggtgcggc
     5581 agtactcggc gccgacgatc gcggccagcg cggcccgatc cgccggcgcc agcgccgacg
     5641 ggcgcagccg cacctggtcg gcgtcgagct gggccttgtc ggaatcctgc aggcccacag
     5701 cgtgcttcag caacgagcgg atgccctcgg aaagcggctt ggccgcggcg ggatctcccc
     5761 acgcgttcca tttcatcggc ggaaggggag cctcggggtg cgtcatgcgt tacagtatta
     5821 cacatgctgt caatcagtaa cgtaccgtcg gacaccggcg accggatcct ggccgcggcg
     5881 gccagctgtg tcgttgattt cggcgtcgac cgggtgaccc tcgccgagat cgcgcgacgc
     5941 gcgggggtga gcaggccgac cgtgtaccgc cgctggcccg acacgaagtc gatcgtggcg
     6001 gcgttgttga ctcagcgcat caccggcgtg atgcgcgatg cgccgctgct cggggatgac
     6061 cgggaatcgc ttgtgcgaca gattgttacg gtcgcggatc tgctgcgtcg ggacgagctg
     6121 gtcatgtcgg tgctgcattc gcagctggca ccgatctaca tcaccgaacg ccttggcgcc
     6181 agccagcaga tgttgatcga cgccctggcc gagcggctgc gggtggcaca gcgacacggc
     6241 agcgtccgcg ccggcgaccc cctgcagatg gccaccgtgg tgttgctgat cactcagtcg
     6301 accattcagt ccgaacagat cgtccggccg atcctcgacg ccgacgcgct ggccaccgaa
     6361 ctcgcctact cgctgaacgg atacctagcg tgaacaccgc tgccctcaac gccgcgcgcc
     6421 gtgcggccga cctgacggcc ctcgccgacg gccagccact cgacgtcctc gtgatcggcg
     6481 gcggcatcac cggcgccggg atcgccctgg atgccgcgtc ccgaggcctg cgggtcgcgc
     6541 tggtggaaaa acacgatctg gcgttcggga ccagtcgctg gagttccaag ctcgtccacg
     6601 gcgggctgcg ctacctggcg accggcgacg tcggaatcgc ccggcgcagc gcgatcgagc
     6661 gcggaatcct gatgacgcgc aacgcccctc atctcgtgca cgcgatgcca cagctggttc
     6721 cgttgctgcc gtcgatgagt cacggcaagc gcgccttggt gcgggccgga ttcctcgccg
     6781 gcgacgcgct gcggatgctg gcgggcacgc cgtcgtcgac actgccgcgg tcgcggaggg
     6841 tctcggcgcg ccacgtggtg gagatggctc ccacggttct acgagatggc ctggacggtg
     6901 gattgctggc ctacgacgga caattgatcg acgacgcccg actggtcacc gcggttgcgc
     6961 gcaccgcggc ccagcacggc gcccggattc tcacgtacct cgcggcgtcc gaagcctcgg
     7021 gaacgtcggc gcggctgacc gatcagcgca cgggagagtc gttcgatgtt tcggccggtg
     7081 ccgtcatcaa cgcggccggg gtgtgggccg gcgagatcga cggctcgttg cggctgcggc
     7141 ccagccgcgg gacccatctc gtcttcgctg cggcggcctt cggcaatccg accgcggcgc
     7201 tgacgattcc gattcccggc gaactcaacc gcttcgtgtt cgccatgcct gagcaactgg
     7261 gccgggtcta tctggggttg accgacgaag ccgctcccgg cccgattccc gacgtgcccg
     7321 agccgtccac cgacgagatc acctttctgc tcgacacggt gaacaccgcg ttggggaccg
     7381 cgttggcgac caccgacgtg atcggcgcct acgccggtct gcggccgctg atcgatgccg
     7441 gcggtgctgg tttcgacggc cgcaccgcag acgtgtcgcg tgagcacgcc gtcgtcgagt
     7501 cggcgtcggg cgtcatcagc gtgatcggcg gcaagctgac cgaataccgc tacatggcac
     7561 aggatgtcct ggatcgtgcg atcagcctgc ggcacctgca cgccggcgac tgccggaccc
     7621 gcaatctgcc gctgatcggg gcaccggcca accccgcacc gttcgcagca agggccgccg
     7681 ggttgccggc gtcactggtg gcgcgctacg gggccgaggc gtccaacgtc atcgccaccg
     7741 ccacctgtga ccggcccggc gaccccgtcg cagagggcat cgacgtcacg cgaggggagt
     7801 tcgaatacgc agtgacgcac gagtgcgcgc tgcaggtcga cgacatcctg gatcggcgga
     7861 cccggatcgg cctggtggcg cgtgaccgcg agcgggttgt cgccgcggcc gaggagttct
     7921 tggcctgaat tcaggactac ctgaccaggg ccgaaatcag cgcacggccc accttcgatt
     7981 ggacgcggga ccgcaacctg taccaggcat cgcccagaac ggctcctgcc gggcggacgg
     8041 actgtggccg attgccaagt cccagaagaa cttccgcggt gtggtgagcg aaacgctccc
     8101 ggtcgtcggg ggagaggtcg catcgctgga ggaactccgt cgcggcgaag ttgtcctgga
     8161 acgggtaatc gacggagaac atcagattgt ccagccccaa catcgctcgg gcacagtcga
     8221 acaccggcac gtcgaacacg ccgctgctgg tgatgaacac gttctttcgc aggtagtagc
     8281 cgacggtgtt gcgcattacg gtgtgcggct catcctgatt gcccgattcg gccgcgagca
     8341 accagcctgc catcgtgagc cctgcgttga gccgggtgaa gcagtaggga agcagctctc
     8401 ccatgtgtcc gacgatgatc ttcaaatcgg ggtgccggtc gaacactccc gcgcagatca
     8461 gcctcagtaa gtgcgttccg gtttccaccg gccagcccca agtcggtacg agggcagaac
     8521 cgaaaggcgc gtaatacacg tccgacaccg ccgccggcgg atcggtggga tgaatgtaga
     8581 tagggacgcc caaggatgcg gctctggcca acagtgggtc gaacgtcggg tcgtcaaggt
     8641 aacgcccgtt ggtgtgtccg ttgatcagcg cacccacgaa cccgagatcg cgcacggtgc
     8701 gctgcaactc gtcggcggcg tgcggcgggg actgggtcgg cagcgtggca aagcccgcga
     8761 accgcgccgg atacgcggcg atcgcatcgg ccagccagtc gttgacttcg cggctcacgg
     8821 caaccgccaa ttcggcctcg tcgtccgcga gaatctggac accgggctga accagtgaga
     8881 gcacctgcac gtcgattccc cccgcgtcca tgagacgaat tcgcccggct ccgacgtcgc
     8941 tgagccgggc cctgacgggc tgatatctgc gctgcaacgg cgggggaaac aaatcccaga
     9001 ctcgctggtg aacaaacgcc tcttccaacg cgaccactcg cgaaaccgac tttgcagaag
     9061 ccattttcgt cacctcctcg actctgctgc t
//
LOCUS       DASSGQ010000199         4044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_955733,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000199 DASSGQ010000000
VERSION     DASSGQ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955733"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..477
                     /locus_tag="VFI55_08810"
     CDS             151..477
                     /locus_tag="VFI55_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074164.1"
                     /translation="MPDTDAISPLTCAWPFAGGEVVAEAPGGLVLELGAVGLDAGLDV
                     VDEPHAAMDSVAAPVAASTAKRVSRGMRELTDNNISPIVGWPDDRLYPLELCPTYVYW
                     NVYSAV"
     gene            complement(474..2315)
                     /locus_tag="VFI55_08815"
     CDS             complement(474..2315)
                     /locus_tag="VFI55_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET7074165.1"
                     /translation="MGHYIANVRDIEFNLFEVLDIGALLGSGAYSDLDEDTVRTILAE
                     AARLAEGPVAETFAFADRNPPVFDPAEHTISVPDELAKTVDAIKEAGWWRLGLTEEIG
                     GMPAPPPLAWAVTEMTFCANPSASFFSLGPLMAQALYVEGNEQQRQWAAMGIERGWEG
                     TMVLTEPDAGSDVGAGRAKAIAQPDGTWHIEGVKRFISGGDVGDTAENIFHLVLARPE
                     GASPGTKGLSLFYVPKYLFDPDTFELGSRNGVFVTGLEHKMGIKSSPTCELTFGATDV
                     PAVGYLVGDVHNGIAQMFTVIEHARMTIGVKAAGTLSTGYLNALAFAKERVQGADLTQ
                     MSDKTAPRVTIIHHPDVRRSLMTQKAYAEGLRALYMYAAAHQDDVVAQHVSGADHEMA
                     HRVDDLLLPIVKGVSSERAYEILTESLQTLGGSGFLVDYPLEQYIRDSKIDSLYEGTT
                     AIQALDLFFRKVVRDRGEALRFVTAQISDTIDSCNDALKPEAELLQTALDDVLAMTAA
                     LTGYLMSATQHPTEIYKVGLGSVRYLLAVGDLLIGWRLLVQGGIAQAALDNLANGNGA
                     KKDTAFYQGKVATAAFFARNMLPKLTAQRRVLEALDDDVMRISESAF"
     gene            complement(2393..3340)
                     /locus_tag="VFI55_08820"
                     /pseudo
     CDS             complement(2393..3340)
                     /locus_tag="VFI55_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394028.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
     gene            3547..3897
                     /gene="trxA"
                     /locus_tag="VFI55_08825"
     CDS             3547..3897
                     /gene="trxA"
                     /locus_tag="VFI55_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET7074166.1"
                     /translation="MTTRDLTAEQFNETIEGNDIVLVDFWASWCGPCRQFAPTFKASS
                     EKHPDVVYAKVDTEAEPQLAAAAQIRSIPTLMAFKKGKLVFNQAGALPPAALEDLVQQ
                     VKAFDVEAAQAEQA"
BASE COUNT          642 a         1359 c         1328 g          715 t
ORIGIN      
        1 ccctcagact cgtcgcggct gggccgcacg gtgacgcagc tgcccgtggt gacgtcggtc
       61 agcgcggccg gagtgacctc ggtgaccttg gtcgacgggg tgaagtccac cgtcgcgctg
      121 ccgttgtccc gctgggtgac ctgaattgcg ttgcccgaca cggacgcgat cagcccgctg
      181 acgtgcgctt ggccgttcgc cggcggcgaa gtggtcgctg aggcccctgg agggctcgtg
      241 ctggaactcg gcgccgtggg actcgatgcg ggactcgatg tggtcgacga gccgcacgcc
      301 gcgatggaca gcgtggcggc cccggtcgcc gcgagcaccg cgaaacgggt gagccgtggc
      361 atgcgagagc tgacagataa caacatttct ccaatcgtcg gatggcccga cgaccggttg
      421 tacccgctgg agctgtgccc gacctatgtg tattggaatg tgtattcggc cgtctagaac
      481 gcgctttcgg aaatgcgcat cacgtcgtcg tcgagagcct cgagtacccg gcgctgcgcg
      541 gtcagcttcg gcagcatgtt cctggcgaag aacgccgcgg tcgcgacctt gccctgatag
      601 aacgccgtgt ccttcttggc gccgttgccg tttgccaggt tgtccagggc ggcctgtgcg
      661 atcccgcctt gcaccagcaa ccgccagccg atgagcaggt cgcccaccgc cagcaaatat
      721 cgcaccgatc cgagccccac cttgtagatc tcggtcgggt gttgcgtggc ggacatgagg
      781 tatccggtca gcgctgccgt catcgccagc acgtcgtcca gcgcggtctg cagcagctcg
      841 gcttccggtt tgagtgcgtc gttgcagctg tcgatggtgt cgctgatctg ggcggtcaca
      901 aaccgcagcg cctcaccccg gtcgcggacg accttgcgga agaacaagtc cagcgcctga
      961 atggcggtgg tgccttcgta cagcgaatcg atcttggaat cgcggatgta ctgctccagc
     1021 ggatagtcga ccaggaagcc ggagccaccc agcgtctgta gcgattcggt caggatctcg
     1081 taggcccgtt ccgagctcac ccctttgacg atcggcagca gcaggtcgtc gacgcggtgt
     1141 gccatctcat gatctgcgcc cgaaacatgt tgtgcgacaa catcatcctg gtgcgcggcg
     1201 gcatacatgt agagcgcccg cagaccctcg gcataggcct tctgggtcat caggctgcga
     1261 cgtacgtcgg ggtggtggat gatcgtgacg cgtggcgcgg tcttgtcgga catctgggtg
     1321 agatccgcac cctgcacccg ttccttggcg aaggccaggg cgttgaggta gcccgtggac
     1381 agggtgccgg cggccttcac gccaatcgtc atgcgagcgt gctcgatcac ggtgaacatc
     1441 tgggcgatcc cgttgtgcac gtcgccgacc agatagccaa cggccggcac atcggtggcg
     1501 ccgaaagtca attcacacgt cggggaggac ttgatgccca tcttgtgttc caggccggtg
     1561 acgaaaaccc cgttgcggga acctagttcg aacgtgtcag ggtcgaataa gtacttgggg
     1621 acgtagaaca ggctcagccc cttggtgccc ggactggcgc cctcgggtcg ggccagtacg
     1681 aggtgaaaga tgttctccgc ggtgtcgccc acgtctccgc cggagatgaa ccgcttgacg
     1741 ccctcgatgt gccaggtgcc gtcgggttgc gcgatggcct tggcgcggcc ggcgccgacg
     1801 tcggatccgg cgtcgggctc ggtgagcacc atggtgccct cccacccgcg ctcgatgccc
     1861 atcgcggccc actgccgctg ttgctcgttg ccctcgacgt aaagcgcctg agccatcaat
     1921 gggcccaggc tgaagaaact cgcggagggg ttggcgcaga aggtcatttc ggtgaccgcc
     1981 caggccagcg gtggcggggc gggcatgcca ccgatctctt cggtcaggcc cagccgccac
     2041 cacccggcct ccttgatcgc gtccacggtc ttggccaact cgtccggaac gctgatcgtg
     2101 tgctcggcag ggtcgaatac cggcgggttg cggtcggcga aggcaaaggt ttcggcgacc
     2161 gggccctccg ccaggcgagc cgcttcggcc agtatcgttc gcacggtgtc ctcgtcgagg
     2221 tcgctgtagg ccccactgcc gagcagggcg cctatgtcga gaacctcgaa caggttgaac
     2281 tcgatgtcac ggacgttggc gatgtagtgg cccactgcca ttccctccgc tcaggctgat
     2341 tcgccttagt ttctccgagg aaggaaaacg tacgcaagcg taactacggc cactacccgt
     2401 tcgcgccgtt ggtgccgaac aggaggccgc gactgccgcc ggtgccgggc gtgctgcccg
     2461 ccgtggcccc tccgccgttg ccgccgttgc cgaccagcag tgcgttgccg ccgttgccgc
     2521 cgctgccggt cgccgtgccg cccctgccgc catccccgcc gttgccgatt aacccggcct
     2581 ggccgccaat cccgccgtta ctggtcgttc cgccgtcgcc gccgccaccg ccgttgccga
     2641 tgaagaaccc gccgttgccg ccacccccgc cgttgccgaa ggagagggag ttcccgcctt
     2701 ggccaccggt gcccagcagc catgccctgc ccccggtacc gccgccggcg aatccggcgc
     2761 cgccggcgcc gccgttgcca taaaggaatc cgccgttgcc gccgccgccg ccggtgccgc
     2821 cgggagcgcc ggcaccgccg agcccgccgt tgccaagcag ccccgcgtcg ccgcctctgc
     2881 cgccagtctg gcccgcggcc gtgccggccc caccagcgcc gccgctgcca aacaagattc
     2941 cgccgtcctc gccggcctgg ccggccgcgc cgttgttgcc gttgccgatc taaggacggt
     3001 tgaatagcgc ccggctgggc gcgttgatta cgtcgaacag cggcttcaag gggttggtgt
     3061 tggcggcctc tgcggtggag tacgcctggg cgccgccgag cagggtctgc acaaattggt
     3121 cgtgaaacgc cgttatctcc gcgctcaccg cctgaaagtt ctggccgtac tcgccgaagt
     3181 aggccgcggc ggcggccgat atctcatcgc cggccgcggc cgccacgccg gtcgtctggg
     3241 tcgccgccgc cgagttggcg gcactgatcg tcgaacctat gcgggccaga ttcccggccg
     3301 catccgacac aagatccgta ctagcgatca cgaacgacat cccgcacctc ccaggtgaag
     3361 cgccagcaac ggaaggatct atcgtgatgc gcagtcacgt ttgtttgttc ttgtcggatc
     3421 catagagcaa ttcggaggcc cggaattcgc ttagccagcg aagccgaccg ataggcggcc
     3481 aagcagcggt ccgagccgca tcgagcccgc gggaacatcg cgccgactcg atgcgttagc
     3541 cgggttgtga caacacgcga tctcactgct gagcagttca acgagaccat cgagggcaac
     3601 gacatcgtgc tcgtcgactt ctgggcctcc tggtgtgggc catgccgcca gttcgcgccc
     3661 accttcaagg cgtcctcgga gaagcacccc gacgtcgtgt acgccaaggt cgacaccgaa
     3721 gccgaaccac agctggctgc ggccgcccag atccggtcca ttcccacgct gatggccttc
     3781 aagaagggca agctggtgtt caaccaggcc ggcgccctgc cgccggcggc gctggaggac
     3841 ttggtgcaac aagtcaaggc gttcgacgtc gaggctgccc aagccgagca ggcctgaccg
     3901 gaaatgacat cgccccggat ttgccagatg cctagtgggg cggtgtcggg gggccttggg
     3961 agttaagcga ttgctgccgc ggtcaatgcg ggcagtgctc gggtgcgatc agaccagttg
     4021 ccgctgaaac tcggcaccgc gacg
//
LOCUS       DASSGQ010000200        15952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_105285,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000200 DASSGQ010000000
VERSION     DASSGQ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15952
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105285"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VFI55_08830"
     CDS             complement(<1..755)
                     /locus_tag="VFI55_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET7074167.1"
                     /translation="MANQVRRGLPLRVGLVAATLLLVACGLAVSGVAVTSILRHSLVS
                     RIDQTLVDASRSWALAPRRQSAPPYEGPDPGRPPSKFYVRGVGTDGTPFTAINDRNAE
                     PALPPDNDVGPYPTTLPSVNGSDIQWRAVSVRGPHGLTTVAVDLSDVQHTLTSLVWLQ
                     IGIGVAVLVVVGIASFAVVHRSLRPLSEVEQTAAAIAAGQLDRRVPERDPRTEVGQLS
                     LALNGMLTQIQEAMASSESSAEKARNSEERMRR"
     gene            complement(808..1527)
                     /gene="phoP"
                     /locus_tag="VFI55_08835"
     CDS             complement(808..1527)
                     /gene="phoP"
                     /locus_tag="VFI55_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HET7074168.1"
                     /translation="MTAAISSETKPEARVLVVDDEANIVELLSVSLKFQGFEVYTATN
                     GAQALDRARDARPDAVILDVMMPGMDGFGVLRRLRADGIDAPALFLTARDSLQDKIAG
                     LTLGGDDYVTKPFSLEEVVARLRVILRRAGKGGAEPRNARLAFADIELDEETHEVWKA
                     GEPVSLSPTEFTLLRYFVINAGTVLSKPKILDHVWRYDFGGDVNVVESYVSYLRRKID
                     TGEKRLLHTLRGVGYVLREPR"
     gene            1688..2410
                     /locus_tag="VFI55_08840"
     CDS             1688..2410
                     /locus_tag="VFI55_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074169.1"
                     /translation="MNFAKGIVSLATAPARLGLAAADASLNIASVAVGVAQRALGGVE
                     GDSATGAVASMLGIEDTIIRANRLARLLDDDAPLGRAVAPDGPMDRLLRPGGVVDMLT
                     SDGGLLDRLTAEGGGLQQALQPGGLVDQLLAEDGLIDRLLAEDGLAVRLLAEGGVVDR
                     LTAKNGPLNQLADAADTLARLTPGMEALEPTISRLQDAVNSLSSLVNPLTNITDLIPI
                     PGRRPRSSSQQVRSTRVIEGDE"
     gene            2437..2508
                     /locus_tag="VFI55_08845"
     tRNA            2437..2508
                     /locus_tag="VFI55_08845"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2469..2471,aa:Thr,seq:tgt)
     gene            complement(2552..3334)
                     /locus_tag="VFI55_08850"
     CDS             complement(2552..3334)
                     /locus_tag="VFI55_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HET7074170.1"
                     /translation="MRDRETIDSELRRIAVGRRSIREQGGQPSSREADELLDELLARS
                     AGASDTDPVKAHETEGAVGTWSRGNKTGTAPRRRKGALRRLGLVAALPLSLVAVVAAA
                     VVMFAVHHHDSSAQPTETPPSAAPPKPIAPDIPAPRIDIVDAAFIAALKHEGVPVPSQ
                     EYVTTQGHAVCDFLAHQPNFADAVGYVQRSSIWDANQSADFTAGAIVSYCPQSQPAAP
                     DQMQPAYQNALSDLQAIQGKLQGIQGDLQGIQGDLEGIPGHP"
     gene            3374..3610
                     /locus_tag="VFI55_08855"
                     /pseudo
     CDS             3374..3610
                     /locus_tag="VFI55_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3774..4712
                     /locus_tag="VFI55_08860"
     CDS             3774..4712
                     /locus_tag="VFI55_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET7074171.1"
                     /translation="MRPAVACVLGPIMVALVGCTAPTAPTTPAAAPPATTVAATPLAS
                     RTVAAAPRPPEGAQPQLASDPAQLADDLVADEQALRDPATAEPALEAAARREQAAYRA
                     IGRHPEWDATTRPRIPPALIDVYDRNVDARRQLTAMAHVKDTLPAWRIEPPAPADELV
                     GYYHAAEADSGVGWNYLAAINFVETHFGSIVGVSTAGAEGPMQFLPSTFASYGQGGDI
                     DSPRDSIMAAGRYLAANGFANDPDHAVFQYNHAHEYVQAVDQYAAVIAADPAAFAAYH
                     RWDVYYNTTAGDVVLPIGYAESSPIPVGDYLAAHPQ"
     gene            complement(4847..5764)
                     /locus_tag="VFI55_08865"
     CDS             complement(4847..5764)
                     /locus_tag="VFI55_08865"
                     /inference="COORDINATES: protein motif:HMM:NF043060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP_0585 family protein"
                     /protein_id="HET7074172.1"
                     /translation="MSMKRVVSAAAVAAGVGISAMTLNPGFANSAPLDPPPPCPNCHG
                     GGSGGGGGGGGGNPGSPGGAPGGGSQNPHAPETPQTTAPPATQSTEPPATHSTAPPSS
                     QSTAPPSSQSPQPSSQSPEPSPARGSEPPRSETTAPSTQTTPPSAQTTSPANGHTTEP
                     FNRPARPVLNPPSTQPPHPPYVPRQDLVTASVQIGGPAGANVQFSIVGRGAPPPPPQR
                     GHGWNDGPAPGHPPPNWEGPPPQGGWDGPPPPGGWNRPWVDRPPRDRDVARADFGPFG
                     YDTFTVIPVFNWQYGGWGFWYFGVWIPLY"
     gene            complement(5799..6269)
                     /locus_tag="VFI55_08870"
     CDS             complement(5799..6269)
                     /locus_tag="VFI55_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LpqH"
                     /protein_id="HET7074173.1"
                     /translation="MKRGIVVGAAGVAVVVAVCAGCSSNKSSTGASSSSAAAAAAGPQ
                     VIVDGQQQTITGQVTCTSANGNTTIGIGDATAGVGAVVSNDNPPIVHSVGLGSVNGVT
                     LGFSDAAPNQGANAGAAISGKSYAIKGTASGVDMSNPQQPQQVTKSFELDATCP"
     gene            complement(6462..7109)
                     /locus_tag="VFI55_08875"
     CDS             complement(6462..7109)
                     /locus_tag="VFI55_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HET7074174.1"
                     /translation="MTMPWLRGLGLVVAVVVAATVSANPAQAEPGVTPGMKVEAINSA
                     CTAGFAAQGNDGSYYLMTSGHCDPHNGSVWSYGNHVQLGRITVSEHDGDARDAAIIML
                     DPRLGPPRGDIGGRYFVRDVLSATQIQRGMPFCKIGAVTGETCGAVKDVQGDVVEASV
                     FSLNGDSGSPGYVKNPDGTVSAVGLLTSSPDGDDYTTYFSLVNPLLSKWGLRILP"
     gene            complement(7289..8164)
                     /locus_tag="VFI55_08880"
     CDS             complement(7289..8164)
                     /locus_tag="VFI55_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7074175.1"
                     /translation="MTTAPEQFSTHGPGGVRIVSDRLGDPQARAVVFLHGGGQTRRSW
                     ARAAAAVASRGWQAVTIDLRGHGDSDWSSEGDYRVVSFARDVEEVLRNLPARPVLVGA
                     SLGGFTSMLLAGEVSPGIASAVVLVDIVPNMEQSGANRIHNFMADRVESGFGSLDEVA
                     DAIAEYNPHRPRPTDLDGLTANLRRRGDRWYWHWDPQFISGAAPHPPFEITDADRMHA
                     AVEAIVCSGVPMLLIRGQVSDLVSQERADEFLARFPQVEFTDVRGAGHMVAGDRNDIF
                     AGAVLDFLARHLDAG"
     gene            complement(8295..8765)
                     /locus_tag="VFI55_08885"
     CDS             complement(8295..8765)
                     /locus_tag="VFI55_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019926598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7074176.1"
                     /translation="MKPDNLYHSGIVVDDLDATLDWFTKVAGYRWTDVVEVDQAAQTP
                     DGELTIPMRIAYSGSAPRVEILQTVPGTVWVPADSGVHHLGYWSDDVEADLATLEANG
                     MRYEVKSYNPDGSGTLLWAYCKGPTGPRIELVSRAMAPFIEYWWSTAHGGSERP"
     gene            complement(8800..10008)
                     /locus_tag="VFI55_08890"
     CDS             complement(8800..10008)
                     /locus_tag="VFI55_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HET7074177.1"
                     /translation="MAQSLDALVRQGEADQDAVDLGDGIFMSKNIANSYLVTTAEGDV
                     LINTGTDFEANAIKARFARVSKRPLKVITFTQGHPDHVGGWDLFHTAGVETIAQANHR
                     DVREYWRRLHPFYTQRIMVLWGAVMDVEARAMKLPPEPALTTTFIDSHTFELGGRRFE
                     LYATPGGETTDALVVWMPDHRTVFIGNLMGPFFGHVPNLYTLRGDKIRSAMAFIHSVD
                     RVIALEPEMLINGHDVFRGANDIRQTMTKVRDATAYLRDRTIDGMNAGNDLWTLMRAV
                     TLPPELALPQVHGKVPWIVRAIWEEHVGWFRYESTTELYDTPPSAVWPDIIELAGGTS
                     ALADRARTHLEDGRALQALHLTDMVLAHSPGDPAALQIKRRALEWLLTNSGRENFSEV
                     QWLEQALGKP"
     gene            10084..11274
                     /locus_tag="VFI55_08895"
     CDS             10084..11274
                     /locus_tag="VFI55_08895"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7074178.1"
                     /translation="MATTHPSLSSQWGPRSDELVRRACERAGLDDFGGDSWREGLQLL
                     VDSVESTPGVNPGGRDFVYGQFVDALWNRLRVVDYLRRHPEVAEERVERPLVILGLPR
                     TGTSLASYLLDQDPQRRSLLTWEAENSVPPSTHESLRADPRCLKKKAELEILAEGLKA
                     ANIPMVHWDEADGPTECVFVQNQDFKSYLWEAFMPNSAYADWLLETDMTSAYAYERSV
                     LQLLQSRAPGTWSLKMPSHAVHIEALLATFPDVRIVWAHRDPFKATASFLRLNYLSRG
                     VLGCEIDTADIVSNVLRQLRAHITRPLKARERIGHDRFFDLHYAALMRDPIRVVRSLY
                     DWAGDELNAPTEQSMLAWLERNPQDRFGVQPYSLDGTGLTRNDLEPIFAEYLSAFEIE
                     LEGV"
     gene            complement(11271..12017)
                     /locus_tag="VFI55_08900"
     CDS             complement(11271..12017)
                     /locus_tag="VFI55_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7074179.1"
                     /translation="MTVVMVHGNPETDAIWGPLVDALGRTDVVRLSPPGFGAPVPVDF
                     AATYLAYRDWLEGELAQIDEPVDVVGHDWGGGHVVNAVMHRPDLVRSWVSDAVGLFDH
                     DYVWHDLAQVWQTPGDGEQLVDTLFGGTLRDRSAQMEALGIPADVATSIAAAQGPEMG
                     RAILSLYRSARQPAMAEAGRTLEKAATRPGLSLLPTDDPYIGPADNRRRAADRAGART
                     EVFDGLGHWWMVQDPARGAAALTRFWESVG"
     gene            12079..12348
                     /locus_tag="VFI55_08905"
     CDS             12079..12348
                     /locus_tag="VFI55_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074180.1"
                     /translation="MRPAQTRHTLSKVPVMIGVVLVLVLAVLAIPVKQRCGAPGYSCA
                     TTLDAQGNVHYYYEVEPLGVYLAESITGSNIRFYYTSGEDLVRVQ"
     gene            12372..12758
                     /locus_tag="VFI55_08910"
     CDS             12372..12758
                     /locus_tag="VFI55_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HET7074181.1"
                     /translation="MDNYAGLAAPAGLIVALVLTFGAPVKDVALVDAHQNVCGAAALC
                     RTDPPPDGGDQATAEQKVIDGYKQKQLNCTPDLPPNPQSLTWDPPGFAPNVGGSGVIH
                     DANPQLGGQYRADYVNGRWHIEYPYC"
     gene            complement(12785..13450)
                     /locus_tag="VFI55_08915"
     CDS             complement(12785..13450)
                     /locus_tag="VFI55_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HET7074182.1"
                     /translation="MADDIDPGSAPGGYRLSAGADVAPPHHRLRQIWAAIPPGPRLVV
                     PIALLLAAVVAGFYAWVKPSRDDWSLLPSRLVCQVQPGPTPPLQLTVASVGVTHPRGN
                     VMQLVVGFKQPLPPSASYEPGYEMKYTLANNGTTFAVLTPQQGTDDLTIGNAKKPGAA
                     GIRTDKENYAAKTAPDTVVISLDLTKFGITEALVSPALSVSSQLDAPPAAPLGYAAQI
                     CHG"
     gene            13705..15252
                     /locus_tag="VFI55_08920"
     CDS             13705..15252
                     /locus_tag="VFI55_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET7074183.1"
                     /translation="MARTGGAHHRHRAARQPSRLRKGLMRGVMTLVSVAAVLLTGAGY
                     YVAHGALGGITVSDALRPDDPRSSGDEMNILLIGLDSRKDQDGNDLPWSVLKYLHAGD
                     SDDGGYNTNTLILVHVGADQKVVAFSIPRDDWVPFNGVPGYNHIKIKEAYGLTKQYVA
                     QKLINQGVSSQKELETRGREAARAATLRAVRSLTGVPIDYFAEINLAGFYDLAQTLGG
                     VEVCLNHPVYDSYSGADFPAGRQRLDASEALAFVRQRHGLENGDLDRTHRQQAFISSV
                     MQELQASGTFTNLDKLKSLMTVARKDVVLSGGWDEDLIQRLGALAGANVEFRTLPVVR
                     YDNIDGQDVNIIDPAAIKAEVAAAIGAPPPTTASATTAAKPSPSTVVDVINAGSMSGM
                     ATEVSSALKKHGYTAGQVRDRDSGEPTATTIVYGAGAETDARNVASLLGLDAPNQPDP
                     GVQPGHIRVTVDTNFSMPAPDDSTADETTTTTTSSKASTYYWSTNTYPTPDQGKPIDG
                     GGVPCVN"
     gene            complement(15284..>15952)
                     /locus_tag="VFI55_08925"
     CDS             complement(15284..>15952)
                     /locus_tag="VFI55_08925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7074184.1"
                     /translation="GLGATRARATDATAAGRLTTVPMDVGNEDSVIDGVARSVQTLGG
                     LDSLVNAAGILRAAHTHETSLELWNQIVGVNLTGTFLVVREALPALLANSRSAVVNFS
                     STSASFAHPYMAAYAASKGGIQAFTHSLALEYARDGLRAVCVAPGSIKSGITDATGGY
                     IPKDADWSLFSRLMPILPTTVESSGAGMADPAVVAGVIAMLVSEDGAFITGTEIRIDG
                     GTHA"
BASE COUNT         2777 a         5280 c         5133 g         2762 t
ORIGIN      
        1 aaccgacgca tccgttcctc ggaattgcgg gccttctcgg ccgaagactc cgacgaggcc
       61 atcgcttcct gaatctgggt cagcatgccg ttcagcgcca acgaaagctg gcccacctcg
      121 gtccggggat cacgttcggg cacccgccga tcgagctggc cggcagcgat cgctgcggcg
      181 gtctgttcga cctcggacag cggccgcagg ctgcggtgca ccacggcgaa gctggcgatc
      241 ccgaccacca cgagtacggc cacgccgatg ccgatctgca accagaccag cgaggtgagc
      301 gtgtgctgga cgtcggacag gtccaccgcc acagtcgtca ggccgtgtgg gccgcgcacc
      361 gacaccgcgc gccactgaat gtcggagccg ttgaccgagg gcagcgtcgt cgggtagggg
      421 cccacgtcgt tgtcgggcgg cagtgccggt tcggcgttgc ggtcgttgat ggcggtaaac
      481 ggggtgccgt cggtgccgac gccgcggacg tagaacttcg acggcggccg gcccgggtcc
      541 gggccttcat acgggggcgc cgactgccgc cgcggggcca atgcccagct gcgtgatgcg
      601 tcgaccagcg tctggtcgat cctgctgacc aggctgtgac gcaggatcga ggtgactgcg
      661 acccccgaga ccgccagacc gcacgccacc aaaagcaggg tggccgccac gagacccacc
      721 cgcagcggca acccgcgccg aacttgattg gccatcctcg tactcttcgc taccgctctg
      781 ccatctcgcc gagtgctgag cgcaggctca gcgcggctcc cgcagcacat aacccacacc
      841 acgcaaagtg tgcagcagcc gtttctctcc cgtgtcgatc ttgcggcgca gatacgacac
      901 gtaggactcg acgacgttga cgtcgccacc aaagtcgtac cgccagacgt ggtcgagaat
      961 tttcggcttg ctgagcaccg tgccggcgtt gatcacaaag tagcgcagca gcgtgaattc
     1021 ggtcggggac agcgagaccg gttcgccggc cttccatact tcgtgggtct cttcgtcgag
     1081 ctcgatatcg gcgaacgcca ggcgtgcgtt tcgcggttcc gcgccgccct tgccggcgcg
     1141 ccgaagtatg acccgcagcc gggcgactac ctcctcgagg ctgaacggct tcgtcacgta
     1201 gtcgtcgccg cccagggtca ggcccgcgat cttgtcctgc agggagtcgc gggccgtcag
     1261 gaacagcgcg ggagcgtcga tgccgtcggc gcggagccgc cgcagcaccc cgaagccgtc
     1321 catcccgggc atcatcacgt cgaggatcac cgcgtcgggg cgggcatcac gggcgcgatc
     1381 cagtgcctgt gccccgttgg ttgcggtata gacctcgaaa ccttggaatt tgaggctgac
     1441 ggagagcagc tcaacgatgt tggcctcgtc gtcgaccacg agcacccgag cctcgggctt
     1501 ggtttcgcta gagatagccg ctgtcatgag gtcacacttc cgtcctttgc tgaaagctac
     1561 cttcccaaac cttgtgggat tcctgaaagc tcggtgccag tcgtctgcta acagtctgcc
     1621 agcaatcatt gcatcagctt ggaacgatac ggcttttgca gggcgcgctg tgcatagact
     1681 cagaactatg aatttcgcga aaggcatcgt gtctcttgcg actgcgccgg cgcgcctagg
     1741 gctcgccgcc gcggacgcga gcttgaacat cgcttcggtg gcggtggggg tggcgcagcg
     1801 agcgcttggt ggagtcgagg gcgactccgc gacgggagcg gtggcttcga tgctcggaat
     1861 cgaagacacg atcattcgcg ccaaccgact ggcgcggctg cttgacgacg acgcgccgct
     1921 gggacgcgcc gttgctcccg acgggccgat ggatcgattg ctgcggccgg gcggggtggt
     1981 cgacatgctc acgtcggacg gcggtttgct tgaccggctg acggcggagg gtggggggct
     2041 gcagcaggcg ctgcagcccg gcggcctagt cgatcagctg ctggccgagg acgggttgat
     2101 cgatcggctg ctcgctgagg acggcctggc ggtgcggctg ctcgccgagg gcggcgtggt
     2161 cgacaggctg accgccaaga acggtcccct gaatcagcta gccgacgcgg cggacaccct
     2221 ggcccggctg acgcctggga tggaagcatt ggaacccacc atttccaggc tccaagatgc
     2281 cgtcaactcg ctctcgtcgt tggtcaaccc gctgaccaac atcaccgacc tcatcccgat
     2341 ccccggccgc cgtccccgct cgtcgtcaca gcaggtgcgc tcaacgcgtg tcatcgaggg
     2401 cgacgagtga ccggttaggc tggcagcgat ttaccggcct ccttagctca gtggtagagc
     2461 actcgccttg taagcgagcg gtcgtcagtt caatcctgac agggggctcg acgcagtaat
     2521 tgccccagcc ggacgaaggc gatgaccagg attaggggtg gcctgggatg ccttccagat
     2581 ctccctggat tccttgcaaa tccccttgga tgccttgcag tttcccctgg atggcttgca
     2641 gatcagagag agcattctga tacgcgggct gcatctggtc cggggccgca ggttgagatt
     2701 gagggcagta agaaacgatg gcacctgcgg tgaagtcggc gctttgattg gcgtcccata
     2761 tcgacgatcg ctgcacgtat ccgaccgcat cagcgaagtt gggttggtgc gctaggaagt
     2821 cgcagaccgc gtggccttgc gtcgtcacgt attcctggct gggaaccggc acgccttcgt
     2881 gtttcagcgc ggcgatgaac gccgcgtcga caatgtcaat ccgcggagcg ggtatatccg
     2941 gcgcaatcgg tttgggcggc gcggccgacg gcggggtttc cgtcggctgc gccgatgaat
     3001 cgtggtggtg gaccgcgaac atcaccaccg ccgcggcgac gacagctacc agagatagcg
     3061 gcaacgctgc gacgaggccc aagcggcgca gcgcaccctt acgtcggcga ggcgcggtgc
     3121 cggttttgtt gccccgcgac caggtgccga cggcaccctc agtttcatgc gctttcaccg
     3181 gatcggtgtc agacgccccg gcgctgcggg ccaggagctc gtcgagcagt tcgtcggcct
     3241 ctcgggacga cggctgaccg ccctgctcgc gaatcgatcg gcgcccaaca gcgatgcgcc
     3301 gcaattctga gtcgatcgtc tcgcggtccc gcatatcaac cagtttgaga gtgtcacgac
     3361 gttgtcacaa gccctggtat acaagcccgg cgcggtggcg gcgacgacgc tccattgggt
     3421 gcgttcgccc ttcgcgtacg tcccgtcgac cggtgtggcg ctggcggttg tggctttctt
     3481 cgggctggcg tacctcacgc tgtggtctgt ttggtactcc cctgggccag cacgtgcgtg
     3541 ggacatggtc accatgaaga accccgatgg cgatgtcgca gacgaacgtt cgagccagct
     3601 gcagcagtaa gtgcttgtcg ggtttcggcg tcaccttgaa ctactgatag ccgccgccgc
     3661 atcgatgtca tatcgtccga tacgaaggac tcgctgcggc tcaccagccg caggaattca
     3721 ggcgctcgac ggaagtgaag ccagtgagcc gtaaccgcgt ggtcgccgcc ccgatgcggc
     3781 cggcggtcgc gtgcgtcctg ggcccaatca tggtggcgct cgtcggctgt acggccccga
     3841 cggcgccgac aactccagcg gccgccccac ccgcgaccac ggtcgctgcc acaccgctcg
     3901 cgtcacgaac ggtggccgcc gcgccgaggc cgccagaggg cgcgcagcct cagctggcgt
     3961 ccgaccccgc acagctcgcc gacgaccttg tcgccgacga acaggcactg cgtgacccgg
     4021 cgaccgccga gccggcgctg gaagcggcgg cacggcgtga gcaggcggcc taccgcgcta
     4081 tcgggcggca ccccgaatgg gatgcgacca cacgaccgcg aatcccaccg gcgttaatcg
     4141 atgtctacga ccgcaacgtc gatgcccggc ggcagctcac cgcgatggca cacgtcaagg
     4201 acacgctgcc tgcgtggcgc atcgagcctc cagctccggc cgacgagctc gtgggttact
     4261 accacgcggc ggaggctgat tccggcgtcg gctggaatta cctggccgcg atcaatttcg
     4321 tcgagaccca cttcggcagc atcgtcggcg tcagcaccgc cggcgcggaa ggcccgatgc
     4381 agttcttacc gtcgacgttc gcttcctacg gccagggcgg cgatatcgac tcaccccgcg
     4441 acagcatcat ggcggcaggt cgctacctcg ccgctaacgg gttcgccaac gatcctgatc
     4501 acgccgtctt ccagtacaac cacgcgcacg aatacgtgca agcggtcgac caatacgccg
     4561 cggtcatcgc agccgatccc gctgcgttcg ccgcctacca tcggtgggac gtctactaca
     4621 acacgaccgc cggcgacgtg gtgctaccca tcggttatgc cgaatcgtca ccgatcccgg
     4681 tcggggacta cctggcggcc cacccgcagt agcaaccagc atctcggttt tcgtcagcat
     4741 ctcggttttc gtcagaaaga agccgccccg accacagcgt cggggcggct ccctgaacag
     4801 gcgcggttga acgtgactag ctcaaccgcg gacggaccgg cgcgcttcag tagagcggaa
     4861 tccagacacc gaagtaccag aagccccagc cgccgtactg ccagttgaag accggaatga
     4921 cggtaaaggt gtcataacca aacgggccga aatccgctcg agcgacgtcc cggtcgcgcg
     4981 gcggcctgtc cacccacggg cggttccagc caccgggagg cggcgggccg tcccacccac
     5041 cctgaggcgg cgggccctcc cagttgggcg gcgggtggcc aggagccggg ccgtcgttcc
     5101 agccgtgccc acgctgcggt ggcggagggg gcgccccgcg gcccacgatg ctgaattgga
     5161 cattcgcgcc cgccgggccg ccgatttgca cgctggcagt gaccaagtcc tggcgcggaa
     5221 cgtacggcgg gtgcggaggc tgcgtgctcg ggggattgag gaccgggcgc gctggccggt
     5281 tgaacggctc tgtggtgtgt ccgttcgccg gcgaggtagt ttgcgcactc ggtggcgtcg
     5341 tttgcgtgct cggagccgtg gtttccgatc tcggtggctc actgccccgc gccggtgaag
     5401 gctccgggct ttgcgacgaa ggctgcggac tttgcgagga cggcggcgcc gtgctttgtg
     5461 aggacggcgg cgcggtgctg tgcgtcgccg gcggctccgt gctttgggtg gccggcggcg
     5521 cagtggtttg cggagtctct ggggcatgcg ggttctgtga gccgccgccc ggtgcgccgc
     5581 cggggctgcc cggattcccg cctccaccgc cgccaccacc accgctgcca ccaccgtggc
     5641 agttcgggca ggggggcgga gggtccagcg gagcggagtt ggcgaacccc gggttcagcg
     5701 tcatcgcgga gataccgacg cccgccgcga ccgccgccgc gctgacgaca cgtttcatag
     5761 acatagctga gttccttctc gggtatcgat cgcctgcact acgggcaggt cgcgtccaac
     5821 tcgaatgact tggtcacctg ctgcggctgc tgcgggttgc tcatatcgac gcccgacgcg
     5881 gtgcccttga ttgcgtagga cttgccgctt atcgcggcac cagcgttggc tccttgattc
     5941 ggagcggcgt cggagaaacc gagcgtgacg ccgttgacgc tgcccagtcc gaccgagtgg
     6001 acgatgggcg gattgtcatt gctgaccacc gcgccgactc cggccgtcgc gtcaccgatg
     6061 ccgatggtgg tgttgccgtt ggccgaggtg caggtgacct ggccggtgat ggtctgctgc
     6121 tgaccgtcga caatcacctg cgggccggcc gccgcggccg ccgccgaact cgatgacgca
     6181 cccgtgctcg acttgttgct cgaacagccg gcacacaccg cgaccactac cgccacgccc
     6241 gctgcgccga ccacgatccc acgcttcaca attgctgctc tccttcgtgt cgctggtgtc
     6301 gctgactcat acatcctggc ctcgacacca cgggccgacc ccaccggccc cttacggaca
     6361 gacgctcacg acgccgcatc gccgggtacc cttttcccca atccgcaaac gttgatcggg
     6421 gcgtcgccgc aggttcgcag attgctgacc agcggtcatc gtcaaggaag aatgcgcaag
     6481 ccccacttgc tgagcagtgg attcaccagt gagaagtacg tcgtgtagtc gtctccgtcc
     6541 ggcgaagacg tcaagaggcc gacagcgctc acggtgccgt ccgggttctt cacgtagccg
     6601 ggactgccac tgtcgccgtt gaggctgaac acgctggctt cgaccacgtc gccctgcacg
     6661 tctttgacgg cgccacaggt ttcaccggtg accgccccga tcttgcagaa cggcatgccg
     6721 cgctgaattt gcgtggcgct caacacatcc cgaacgaaat accggccgcc gatgtcgcct
     6781 cggggcgggc cgaggcgtgg atcgagcatg atgatggcgg cgtcccgcgc gtcgccgtcg
     6841 tgctcgctga cggtgatcct gccgagctgg acgtgattcc cgtaggacca caccgaaccg
     6901 ttgtgcgggt cacagtgtcc actcgtcatg aggtaatagc tgccgtcgtt gccttgagcc
     6961 gcgaaacccg ctgtgcacgc gctgtttatc gcctcgacct tcatgcccgg cgtcacaccc
     7021 ggctctgcct gtgccgggtt cgccgacacc gtggctgcca cgaccacagc aacaaccagc
     7081 cctaaccctc gcaaccaagg catggtcacg ccgcccctct ccttgtgtag ggcagcatgg
     7141 taacagcgcg aaaagggttt ctcctgccga cggcaacgcc ctcgtcacac gcctaaccag
     7201 gcactcggaa caaatattag gaccagtcga ccaaggctgg atcatcccac ggcagcgctg
     7261 ccgatggcgc gcggtcgacg cgagtttgct aaccggcgtc gaggtgacgg gcgaggaaat
     7321 ccagtacagc gccggcgaag atgtcgttgc ggtcgccggc gaccatgtgc ccggcgccgc
     7381 gaacatcggt gaactcgacc tgcgggaagc gcgcgagaaa ttcgtcagcg cgttcctggc
     7441 tgacgaggtc gctcacctga ccccggatca acagcatcgg cacgccgctg cacacgattg
     7501 cctcgacggc agcgtgcatg cgatcggcgt cggtgatctc aaagggggga tgcggcgcgg
     7561 cgccgctgat gaactgggga tcccaatgcc agtaccagcg atcgccccgg cggcgcaggt
     7621 tagcggtcag tccatccaaa tcagtcggtc ggggccgatg tgggttgtac tccgcgatcg
     7681 cgtccgcgac ctcgtcgagc gaaccgaacc ccgattcgac ccggtcggcc atgaagttgt
     7741 gtatccgatt cgccccggat tgctccatgt tcggcacgat gtcgaccaac acgaccgcgc
     7801 tggcaatgcc cggcgagacc tcgcccgcca gcagcatcga ggtgaagcca cccagagatg
     7861 cacccaccag gaccggtcgc gcgggcagat tgcgcagcac ttcttcgaca tcgcgcgcaa
     7921 agctgacgac acggtagtcg ccttcgctcg accagtccga gtcgccatgg ccccgcaggt
     7981 cgatcgtgac ggcctgccac ccacggctcg cgacggcggc ggcagcccgg gcccatgatc
     8041 ggcgcgtctg cccgccgccg tgcaggaaca cgacggcccg cgcctgcgga tcgcccaggc
     8101 gatcagacac gatgcgaacg ccgcctggac catgggtgga gaactgttca ggtgcggtcg
     8161 tcatcagcag atactaagac gctctcactt actgcccgga acgcgcgagg cgacattcgc
     8221 tcccgggtga gcaggctgat ctcgatcgag aacataacct ccgcacctcc gcccgaagat
     8281 cggtgcagcc agcgtcaagg ccgttcagac ccgccgtgag ccgtcgacca ccaatattcg
     8341 ataaagggcg ccatcgcccg actcaccagc tcaatgcgcg gtccggttgg ccccttgcag
     8401 taggcccaca gcagcgtccc ggatccgtcc gggttgtacg acttgacctc ataccgcatg
     8461 ccattggcct ccaatgtcgc caggtcagcc tcgacgtcgt cggaccagta gcccagatgg
     8521 tgtacgcccg aatccgccgg aacccagaca gtgcccggta cggtctgcag gatctcaaca
     8581 cgcggcgcgc tacccgaata cgcaatccgc atcggtatgg tcagctcgcc gtcgggggtc
     8641 tgggcggcct gatccacctc gactacgtcc gtccagcggt agccggccac tttggtgaac
     8701 cagtccaggg ttgcgtcgag gtcgtcgacg acgatgccgc tgtggtagag attgtctggc
     8761 ttcactgggt ttcctcagtt tccggggttc tggtggtctt cagggtttgc ctaaggcctg
     8821 ctcgagccac tgcacctcgc tgaagttctc gcggccgctg ttcgtcagta gccattcgag
     8881 tgcgcggcgc tttatctgca aggcagcggg gtcgcccggt gaatgggcaa ggaccatatc
     8941 ggtgaggtga agcgcctgca gtgcgcgtcc atcctcgagg tgggtacgcg cacggtcagc
     9001 gagtgcgctt gtgccgccgg ctaattcgat gatgtctggc caaaccgccg atggtggagt
     9061 gtcgtagagc tcggtggtcg actcgtagcg gaaccatccg acgtgctcct cccaaatcgc
     9121 ccgcacgatc cacggcacct tgccgtgcac ctggggcagt gcgagttcgg gtggaagcgt
     9181 gactgcgcgc atcaacgtcc aaaggtcgtt gccggcgttc attccgtcga tggttcggtc
     9241 gcgcagatac gcggtagcgt cacgcacctt ggtcatggtc tgccgaatgt cgttggcacc
     9301 gcggaatacg tcgtgtccgt tgatcagcat ttccggttcc agcgcgatga ctctgtcgac
     9361 cgagtggatg aaggccatgg cgctacgaat cttgtcgccc cgcaacgtgt atagattcgg
     9421 cacgtggccg aagaacggcc ccatcaagtt gccgatgaag acggtgcggt gatctggcat
     9481 ccagacgacg agggcgtcgg tggtctcgcc gccgggcgtc gcgtagagct cgaaccgccg
     9541 accgccgagt tcgaatgtgt ggctgtcgat gaacgttgtg gtgagcgcgg gttccggcgg
     9601 cagcttcatc gccctcgcct cgacatccat gaccgcaccc cagagcacca tgatgcgctg
     9661 ggtatagaag ggatgcaggc gtcgccagta ctcacgcacg tcgcgatggt tcgcctgggc
     9721 gatcgtctcc acaccggccg tgtggaacaa atcccacccc ccgacgtggt cgggatgccc
     9781 ctgggtgaaa gtgatgacct ttaatggccg cttgctcacc cgagcgaacc gcgccttgat
     9841 cgcgttcgcc tcgaaatcgg tgccggtgtt gatcaacacg tcgccctcgg cagtggttac
     9901 caggtaactg ttggcgatgt tcttggacat gaagattccg tcgccgaggt cgacggcatc
     9961 ctgatcggcc tcaccttgtc tgacgagcgc atctaaggat tgcgccatcg agccggcctc
    10021 ctcgagaagg gttccgtcga caacctaaca tttgttccac gcggacgtat agtctggtcg
    10081 ggcatggcga caacacaccc atcactctca tcgcagtggg gtccgcggtc tgacgaactc
    10141 gtgcgccgcg cttgcgagcg tgccggtctc gacgacttcg gcggtgattc gtggcgcgag
    10201 ggccttcagt tgctcgtcga cagcgtcgaa tccacgccgg gagtcaatcc cggcggccgc
    10261 gacttcgtgt acggccagtt tgtcgacgcg ctgtggaacc gcctgcgcgt cgtcgactac
    10321 ctgaggcgac accccgaggt cgccgaggaa cgggtggagc gaccgctggt gatccttggg
    10381 ctgccacgta ccggcacgtc gctggcgagc tatctgctcg accaagatcc acaacggcga
    10441 tcgctcctga cctgggaggc cgagaactcg gtgccgccct cgacgcacga gtcgctgcgc
    10501 gccgatccac gctgcctgaa aaagaaggcg gaactcgaga tcctcgccga agggctcaag
    10561 gcggccaaca tcccgatggt gcattgggat gaggccgacg gaccaaccga gtgcgtattc
    10621 gtgcagaacc aggatttcaa gtcctatctc tgggaggcgt tcatgcccaa ctcggcttac
    10681 gcggattggc tcctggagac cgacatgaca agcgcctacg cctacgagcg gtcggtgttg
    10741 cagctgttgc agtctcgtgc ccccggcacc tggtcgctca agatgccgtc gcatgcggtg
    10801 cacatcgagg cactcttggc gacatttccc gacgtgcgca tcgtgtgggc acaccgcgac
    10861 ccattcaagg ccaccgcatc gtttctgcgg ttgaactacc tgtcccgggg ggttctcggc
    10921 tgtgagatcg acaccgctga catcgtgtcg aatgtgttgc gccagttgcg cgcacacatc
    10981 acccggccgc tgaaagctcg cgaacgcatt ggccacgacc ggttcttcga cctgcactat
    11041 gcggccctca tgcgcgaccc catccgcgta gtgcgctcgc tgtatgactg ggccggcgac
    11101 gaactgaatg ccccgacgga gcagtccatg ctcgcttggc tcgaacgaaa tccgcaggac
    11161 cgcttcgggg ttcagccgta ctcgctcgac gggaccggct tgacccggaa cgacctcgaa
    11221 cccattttcg ccgaatattt gtcggccttc gagatcgaac tcgaaggcgt ttagccaacc
    11281 gattcccaga agcgggtcag agccgccgca ccgcgcgccg gatcctgcac catccaccag
    11341 tgcccgagtc cgtcgaacac ctcggtgcgg gcccctgccc ggtcggcggc gcggcgccgg
    11401 ttatccgcgg ggccgatgta tggatcgtcg gtgggcagca acgaaagtcc aggccgggtt
    11461 gcggccttct ccagagtgcg gccggcttca gccatcgccg gctggcgcgc cgagcggtac
    11521 aacgacagga tcgcgcgacc catctccggg ccctgcgccg cggcgatcga cgtcgcgacg
    11581 tccgcgggga tgcctaacgc ctccatctgt gccgagcgat cccgaagcgt gccgccgaac
    11641 agcgtgtcga ccagttgttc gccatcgccc ggggtctgcc acacctgcgc gaggtcatgc
    11701 cagacatagt cgtggtcgaa cagcccgacg gcatcgctga cccaactgcg cacgaggtcc
    11761 ggtcgatgca tcaccgcgtt caccacgtgg ccgccgcccc agtcgtgccc gacgacgtcg
    11821 accggttcgt cgatttgcgc caactcaccc tcaagccagt cgcggtaggc caggtacgtc
    11881 gccgcgaagt ccaccggcac tggagccccg aatcccggcg gcgacagccg caccacatcg
    11941 gtgcgcccaa gggcatcgac caacggcccc cagatggcgt cggtctcggg attgccatgc
    12001 accataacca cggtcatggt gaacatcctg acaccgcacg aacggctgat tgtcgtgccc
    12061 ggcgattaca ctcctgtgat gcgtccggcg caaacgaggc acaccctgag caaggtccca
    12121 gtcatgatcg gggtcgttct cgtgctggtg ctcgccgtcc tcgccatacc ggtcaagcaa
    12181 cggtgtggtg cgcccggcta ctcgtgcgcg acgacgctgg atgcgcaggg caatgttcac
    12241 tactactacg aggtcgaacc gctcggcgta tatctcgctg aaagcatcac gggctcgaat
    12301 attcgtttct attacacgtc cggcgaggac ctggtaaggg tccaatagcc gcaggggctg
    12361 tgggggagaa catggacaac tacgccggcc ttgccgcacc tgcaggactg atcgtcgcgc
    12421 tcgtgctgac gttcggcgca cccgtcaagg acgtggccct cgtcgatgca caccaaaacg
    12481 tctgcggcgc ggccgctctg tgtcgcaccg atccgccacc cgatggcggt gatcaagcca
    12541 ccgcggagca gaaggtcatt gacggctaca agcaaaaaca gctcaattgc accccggacc
    12601 tgccacctaa tcctcaatcc ctcacctggg atccccctgg cttcgcgccc aacgtcggcg
    12661 gatctggcgt catccacgac gccaatccac agctgggtgg gcagtaccgc gccgactacg
    12721 tgaacggccg atggcacatc gaatatccct actgctgaaa tgacggcgcg ccaggacgga
    12781 ttgcctagcc gtgacagatc tgcgccgcgt atccgagcgg cgctgccggc ggcgcatcca
    12841 gctgagagga gacggaaagc gccgggctca ccaacgcctc ggtgatcccg aatttggtca
    12901 ggtcgaggct gattacgacg gtgtccggcg cggttttggc ggcgtagttc tccttgtcgg
    12961 tccggatgcc tgcggccccg ggcttcttgg cgttgccgat cgtcagatcg tcggtgccct
    13021 gctgcggggt caggacagcg aacgtcgtgc cgttgttcgc gagggtgtac ttcatttcgt
    13081 agcccggctc gtagctcgcc gatggcggca gcggctgctt gaatccgacc accagttgca
    13141 tcacattgcc gcgggggtgc gtgacgccca ccgaggcgac agtcaattga agcggcggcg
    13201 tcgggcccgg ctgcacttga caaacaagac ggcttggcaa tagtgaccag tcgtctcgcg
    13261 acggcttcac ccacgcatag aagccggcga cgacggcggc cagcaacaag gcgatgggaa
    13321 ccaccaatcg cggtccgggc ggaatcgccg cccagatctg ccgtaagcgg tggtggggag
    13381 gtgccacatc ggcaccagct gacaagcggt atccgcccgg tgcagagccg gggtcgatat
    13441 catcagccac gtgttcctcc tgagacctcc ccgacatgct cccggaggag aagttcggcg
    13501 aatcttagcg aaaacgtgct ggtagcggga cggtcgaggc catgaaattc acctctccag
    13561 gcgggtgagc ctgcgcacat cgacgttcgc agttggaggg ttcacggcaa cctaccgcat
    13621 acttgacggc atgctgcaag cgacatcacc gcaaacgacg accgcggtaa tgggcgctgc
    13681 gctggtacga agacgaggat cttgatggcg cgcactggcg gtgcccatca tcgtcatcgc
    13741 gccgctcgac agccgtcgcg tcttcgcaaa gggctgatgc gtggcgtgat gacgctggtg
    13801 tcggtggcag ccgtgctgct gaccggggcg ggctattacg tggcccacgg cgctctgggc
    13861 ggcatcaccg tgtccgacgc gctgcggccg gacgatccgc gatccagcgg cgacgagatg
    13921 aacatcttgc tcatcgggct ggactcgcgg aaagaccagg acggcaacga cctgccctgg
    13981 tcggtcttga agtatttgca cgccggcgat tccgacgacg gtggctacaa caccaacacc
    14041 ctgattctgg ttcacgtcgg cgccgatcag aaggtcgtcg ccttttccat accgcgcgac
    14101 gactgggtgc ccttcaacgg cgttcccgga tacaaccaca tcaagatcaa agaggcatac
    14161 gggcttacca agcaatacgt cgcgcagaaa ctcatcaacc agggtgtcag cagtcagaaa
    14221 gaactcgaga cgcggggccg tgaagcggcc cgcgccgcaa ccttgcgggc ggtgcggagc
    14281 ctgaccggcg ttccgatcga ctacttcgcc gagatcaacc tggccggctt ctacgacttg
    14341 gcccaaacct tgggcggcgt ggaggtgtgc ctgaaccacc ccgtctacga ctcgtactcg
    14401 ggtgccgact tcccggccgg gcgccagagg ctggacgcct cggaggcgct ggcgtttgtc
    14461 cggcaacgtc acggcctgga aaacggcgac ctggaccgca cccaccggca gcaggccttc
    14521 atctcgtcgg tcatgcagga actgcaggct tcgggcacct tcaccaacct ggacaagctg
    14581 aagagcctga tgacggtggc gcgcaaggac gtggtgctgt cagggggttg ggatgaggac
    14641 ctcatccagc ggctgggtgc gctggccggg gcgaacgtcg aattccggac actgcccgtt
    14701 gtgcgctatg acaacatcga cggccaggac gtcaatatca tcgacccggc cgcgatcaag
    14761 gccgaggtgg cggctgcgat cggcgcccct ccgccgacga ccgcatccgc caccaccgcg
    14821 gccaagccca gcccgtccac ggtcgtcgac gtgatcaatg ccggcagcat gagcggaatg
    14881 gccaccgagg tgtccagcgc gctgaaaaag cacggctata ccgcgggtca ggtccgcgac
    14941 cgtgattcgg gtgagccgac cgccaccacg atcgtgtacg gcgcgggcgc ggaaaccgac
    15001 gcacgtaacg tggccagtct gctcggcctc gacgccccca accaaccgga tcccggtgtc
    15061 cagcccggac acatccgcgt gacggtggat accaacttct cgatgccggc gccggacgac
    15121 agcacggcgg acgaaacgac gaccaccacg acgtccagca aggccagcac gtattactgg
    15181 agcaccaaca cctacccgac gcccgatcaa ggaaagccga tcgacggcgg cggggtgccg
    15241 tgtgtcaatt aggcgcgcaa gcacgcggaa tcaatcaggc gcctcaggcg tgggtgcccc
    15301 cgtcgatccg gatttcggtg ccggtgatga atgcgccgtc ctcggacacc agcatcgcga
    15361 tcactccggc gaccaccgcg gggtcggcca tgcccgcgcc gctggattcc accgtggtgg
    15421 gcaggatcgg catcaacctc gaaaacagtg accagtcagc gtctttcgga atgtagccgc
    15481 ccgttgcatc ggtgatcccg gacttgatac tgcccggcgc cacgcacacc gcgcgcaggc
    15541 cgtcgcgcgc gtactcgagc gccaaggaat gggtgaaggc ctggattcca cccttgctcg
    15601 ccgcgtaggc cgccatgtag ggatgcgcga aagacgccga tgtggaactg aagttcacga
    15661 ccgcgctgcg cgaattcgcg agcagcgccg gaagcgcttc ccggaccacc agaaaggttc
    15721 cggtcaggtt gacgccgacg atctgattcc acagttccag gctggtctcg tgggtgtggg
    15781 cggcgcgcaa gataccggcc gcgttgacca gcgaatccag gccgccgagc gtctggaccg
    15841 accgggccac gccgtcgatc accgaatcct cgttgccgac gtccatcggc accgtcgtga
    15901 ggcggcccgc ggctgtcgcg tcggtcgccc gggcccgagt ggcgcccagg cc
//
LOCUS       DASSGQ010000201         5108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_531108,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000201 DASSGQ010000000
VERSION     DASSGQ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531108"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /gene="ald"
                     /locus_tag="VFI55_08930"
     CDS             complement(<1..1055)
                     /gene="ald"
                     /locus_tag="VFI55_08930"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET7074185.1"
                     /translation="MRVGVPTETKTNEFRVAITPAGVAELTDRGHEVLVQAGAGEGSA
                     ITDAEFKAAGAQLTSTAEPVWAAADLLLKVKEPIPAEYGLLRRGQTLFTYLHLAASHA
                     CTDALLSSGATSIAYETVQAGDGTLPLLAPMSEVAGRLSAQVGAYHLMRSQGGRGVLM
                     GGVPGVKAADVVVVGAGTAGYNAARVAGGMGASVMVFDVNIPKLRLLDAEFGGHIATR
                     HSSAYELEGAVKRADLVIGAVLVPGARAPRLISNSLVAQMKPGAVLVDISIDQGGCFE
                     DSRPTTHDHPTFAVHDTLFYCVANMPSAVPKTSTFALTNATMPYVLKLADHGWQAACR
                     SDPALAKGLSTHEGMLLS"
     gene            1123..1638
                     /locus_tag="VFI55_08935"
     CDS             1123..1638
                     /locus_tag="VFI55_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET7074186.1"
                     /translation="MPDRLTERKPRPDGRPKDVLPAGLDEVDRKILTLLHNDARIPNN
                     ALAEAVGIAPSTCHGRVRRLVDLGVIRGFYTDVDPVAVGLPLQAMISVNLQSNARGKI
                     RNFIQQIRSKRQVMDVYFLAGADDFILHVAARDTDDLRSFVVENLNADADVAGTQTSL
                     IFEHLRGAAPI"
     gene            1815..3023
                     /locus_tag="VFI55_08940"
     CDS             1815..3023
                     /locus_tag="VFI55_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7074187.1"
                     /translation="MTQVLRAQVCVVGGGPAGLVHALLLARAGIRVVVLEKHNDFLRD
                     FRGDTVHPSTLRIIDELGFIDEFLRLPHTKIDRVAFDTDGSIRTFGNFSALKWLGFKQ
                     PYIALMPQWDFLDFLAEKASDYPEFTLVRNAEVKDLIFDGDRVSGVRTPELEVRAELV
                     VAADGRKSAVRAASGLGLASAHSPMDVLWFRMKWRPGDPQELFGVMRKGLLMAMIYRG
                     DYWQVAYLMPKGANPRGGSLEAFKERLVAARPQLREHVNDLRTWDDTSQLDVRVDRLK
                     TWWRAGLLCIGDAAHAMSPAGGVGINLAVADAVAAANILCAPLREGRLTDADLAKVQR
                     RRELPARIIQAGQVFIQNRAVEPNLNGDLSTIRVPRIVGWGPLQFVPPIVVGRGFRPE
                     HVRTPDVHAF"
     gene            3135..4043
                     /locus_tag="VFI55_08945"
     CDS             3135..4043
                     /locus_tag="VFI55_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015630547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET7074188.1"
                     /translation="MFTVDDQAAGPHDASLDHERLVLEARDVEFDWAKLPFHYVPDEP
                     FATHVLNVLHLLLPAGEEFFVDVFKKTLPLIKDDQLRLDVQGFIGQEATHSRAHSKVL
                     AHFDAQGVDLTPYTDQIKWLFDKLLGPRPGWSLRRQHSWLLEQVSFVSAIEHYTAILG
                     EWVLNSPAHDGIGTDPVMLDMLRWHGAEEVEHKAVAFDTMKHLRAGYWRRVRAQLVVT
                     PAMLLLWIRGVRFMYAVDPYLPPGARPRWRDYFRAARRGLVPSPLRFVRVVGDYYRPG
                     FHPSQLGGLGLAVDYLALSPAARASH"
     gene            4047..>5108
                     /locus_tag="VFI55_08950"
     CDS             4047..>5108
                     /locus_tag="VFI55_08950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HET7074189.1"
                     /translation="MAAGDYAVAQSIWASRPTDLYGRRDRDRLGTVVWGIGVLFGGLA
                     STSRWRPSRVKPVRRTQSAVVVKREVVAPDVVALTLADPGGGLLPSWTPGGHIDVALP
                     SGRRRQYSLCGPPGGRTDYRIAVRRIADGGGGSIEMHDAYGVGDTVEFEGPRNAFYLG
                     TAERDVLFVIGGIGVTPMLPMIHVAAQRGIDWRAIYAGSSREFMPFLDEVVAVDPDRV
                     RVWADDEHGRFATVDDLLSGAGATTAVYVCGPTAMLEAVRVARDEHADAPLHYERFSP
                     PPVVDGIPFELELARSQRVLAVPANRSALDIMLDRDPGTPYSCRQGFCGTCKVKVLAG
                     QVDHRGRTAVGDDEMLVCVS"
BASE COUNT          838 a         1629 c         1724 g          917 t
ORIGIN      
        1 gacagcagca tcccctcgtg cgtcgaaagc cctttggcca gagcgggatc cgaacggcat
       61 gccgcctgcc agccgtggtc ggcgagtttg agcacatacg gcatcgtcgc attggtcaaa
      121 gcgaaggtgg acgtcttcgg caccgcgctg ggcatgttcg ccacgcagta gaacagcgtg
      181 tcgtgcacgg cgaacgtcgg gtggtcgtgg gtggtgggcc gggaatcctc gaagcaaccg
      241 ccctggtcga tggagatgtc caccagcacc gcacccggct tcatctgtgc gacaagcgaa
      301 ttcgagatca gcctgggcgc cctggcgccg ggcaccaaaa cggcgccgat caccaggtcg
      361 gcgcgtttca ccgcgccttc gagctcgtag gccgacgagt ggcgagtggc gatgtgaccg
      421 ccgaactccg cgtccagcag tcgcagtttg ggaatgttga cgtcgaagac catcacggac
      481 gcgcccatgc cgcccgctac ccgcgcggcg ttgtagcccg ccgtgccggc gccgaccacc
      541 acgacatcgg cggccttgac acccggcacc ccgcccatca gcacaccgcg gccgccctgg
      601 gaccgcatca ggtggtaggc cccgacctga gcggacagcc gacccgcgac ctcgctcatc
      661 ggagcaagta acggaagcgt gccgtcgccg gcctggacgg tctcgtaggc gatcgacgtt
      721 gcgccggagg acaacagcgc atcggtgcag gcgtgcgagg ctgccaaatg caggtaggtg
      781 aacagggtct ggccgcgtcg cagcagaccg tactcggccg gtatcggctc tttgaccttg
      841 agcaacaggt cggcggcggc ccacaccggc tccgcggtgc tggtcagctg ggcgccggcc
      901 gccttgaact ccgcgtcggt gatcgccgac ccttctccgg caccggcctg gaccagcacc
      961 tcgtggccgc ggtccgttag ctcggcgacg ccggcagggg tgatggccac cctgaattcg
     1021 ttggtcttgg tctcggtggg aacgccaaca cgcatggctc aagtgtgaag aagattcggg
     1081 caggctgcaa tgaagctgat gacaattctg tagagtcgtg tcgtgcccga taggctaacg
     1141 gagcggaagc cccggcccga tggccggccg aaggatgttc tacccgccgg tctcgatgag
     1201 gtggaccgta agatcctgac cttgctgcac aacgatgccc gcatccccaa caacgccctc
     1261 gcggaggcgg tcgggatcgc gccgtcgacg tgtcatggcc gggtccggcg gctggtggac
     1321 ctcggtgtca tccgggggtt ttacaccgac gtcgatccgg tcgcggtggg tttaccgctg
     1381 caggcgatga tctcggtgaa cctgcagtcc aatgcccggg ggaagatccg caacttcatc
     1441 cagcagatcc gtagcaagcg tcaggtgatg gacgtctatt ttctggcggg cgcagacgat
     1501 ttcatcctgc acgtcgccgc gcgtgatacc gacgacctgc gctccttcgt cgtcgagaac
     1561 ctcaatgccg acgccgacgt ggcgggtacc cagacgtcgc tgatcttcga gcatctgcgt
     1621 ggggccgcgc cgatctagag aacccatgcc aaaaccgggg tggactcagc gacgggagcg
     1681 tcggatcgga cacgctgacg cgacaagcgt ccgtcaactc cctactgaag tgcgtaacaa
     1741 ttcgtaacga tgaccaacat tcggccgcag agctacgccg tcgttgtgtc acgcgacgcc
     1801 aaggacttcg ccgcatgaca caagttctgc gggcccaggt ttgtgttgtc ggtggcggtc
     1861 ccgccggact ggttcacgca cttttgcttg cccgcgccgg gatccgggtt gtcgtcttag
     1921 agaagcacaa tgacttcctg cgtgacttcc gcggcgacac cgtgcatccg agcaccctgc
     1981 ggatcatcga cgagttggga tttatcgatg agttcctgcg tctaccgcac accaagatcg
     2041 atcgtgttgc cttcgacacc gatggctcga ttcgcacctt cggcaacttc agtgcgctca
     2101 aatggcttgg cttcaagcag ccgtatatcg ctttgatgcc gcagtgggac ttcctcgatt
     2161 tcctcgctga aaaggcttcg gactacccgg aattcactct ggttcgcaac gctgaggtca
     2221 aggacctgat cttcgacggc gatcgggtga gcggggtgcg cacgccggaa ctggaggtgc
     2281 gggccgagtt ggtggtggca gcggacgggc gcaagtccgc ggtgcgggcc gcctctggtc
     2341 tggggctggc cagtgcccac tccccaatgg acgtgctgtg gttccggatg aaatggcgcc
     2401 ccggcgatcc gcaggagctg ttcggggtga tgcggaaggg cctgctcatg gccatgatct
     2461 accgcggcga ctactggcag gtcgcgtatc tgatgccgaa gggagcaaat ccgcggggcg
     2521 gctcgctgga ggcgttcaag gagcgcctgg ttgctgcccg gccgcagctg cgtgaacacg
     2581 tcaacgatct acgtacctgg gacgacacca gccagctcga cgtgcgggtg gaccggctca
     2641 agacctggtg gcgggcgggc ctgctatgca tcggcgatgc cgcgcacgcc atgtcacctg
     2701 ccggtggggt cggcatcaac cttgccgttg ccgatgcggt ggccgcggcc aacatcctct
     2761 gcgctcccct gcgcgaaggt cgattaactg acgcggacct ggccaaggta cagcgccgtc
     2821 gagagctgcc cgcccggatc atccaagctg gccaggtctt cattcagaac agggcagtcg
     2881 agcccaattt gaacggtgac ctgtccacta tccgggtccc gaggatcgtt ggatggggcc
     2941 cgctgcagtt cgtgccaccg attgtcgtcg gacggggctt ccgtcccgag catgtccgca
     3001 ctccggacgt acacgccttc tagcgggagg catcgtcgcc gccagtggac gaccgggttt
     3061 tatacttggc agcgctcgat gtcgtctaag tgacattcgg cagatcggtg taaacgccga
     3121 gctagggggc cacgatgttt acagttgacg accaggcggc cggtcctcac gacgcttcgt
     3181 tggaccacga gcgtctcgtc ctggaggcgc gcgatgtcga attcgactgg gcgaagttgc
     3241 cgtttcacta tgtgcccgac gagccgtttg ccacccacgt cctcaacgtg ctgcacctgc
     3301 tgctgcccgc cggcgaagag ttcttcgtcg acgtgttcaa gaagacgttg ccgttgatca
     3361 aagatgacca gctgcggctg gatgtgcagg gcttcatcgg tcaggaggcc acgcattccc
     3421 gggcgcactc gaaagtgctc gcccatttcg atgcccaggg cgtcgacctg accccctata
     3481 ccgaccagat caaatggctg ttcgacaagc tgctggggcc caggcccggg tggagcctgc
     3541 gacggcagca cagctggctg ctggaacagg tttcgttcgt gtcggcgatc gagcactaca
     3601 ccgccatatt gggggagtgg gtgctcaact cgcccgccca cgacgggatc ggcaccgatc
     3661 cggtgatgct ggacatgctg cggtggcacg gtgccgaaga agtcgagcac aaggcagtcg
     3721 ccttcgacac catgaagcac ctgcgggccg gatattggcg ccgggtgcgc gcccagctgg
     3781 tcgtcacgcc cgccatgctg ctgttgtgga ttcgcggcgt gcgcttcatg tacgcggtgg
     3841 atccttacct gccgccgggg gccaggccgc gctggcggga ctacttccgg gcggcacgcc
     3901 ggggattggt gccctcaccg ctgcgattcg tgcgggttgt cggggactac taccggccgg
     3961 gcttccaccc gtcccaactg ggcgggctgg ggctggcggt cgactacctc gcgctctcgc
     4021 ccgccgcccg cgcctcacac tgatcgatgg cggcggggga ttacgctgtg gcgcagagca
     4081 tctgggcgag caggcccacc gacctctacg gcaggcgcga ccgcgaccgc ctcggcacgg
     4141 tggtgtgggg cattggcgtg ttgttcggag gactcgcctc gacctcgcgg tggcggccgt
     4201 cgcgggtgaa gccggtgcgg cgcacgcaga gcgccgtggt cgtcaagcgc gaagtcgtcg
     4261 ccccggacgt ggttgccctc acgttggccg acccgggtgg cggattgctc ccgtcctgga
     4321 cgcccggcgg gcacatcgac gtcgcgctgc cgtcgggacg acgccgccag tactcgctgt
     4381 gcggtccgcc cggagggcgc acggactatc gcatcgccgt ccgccggatc gccgacggcg
     4441 gcggcggttc cattgagatg catgacgcct acggcgtggg agacaccgtc gagttcgaag
     4501 gcccgcgcaa tgcgttctat ctcggcacgg ccgagcggga cgtgctgttc gtcatcggcg
     4561 gcatcggcgt gacacccatg ctgccgatga tccacgtggc tgcgcagcgc ggaatcgatt
     4621 ggcgcgcaat ctatgccggg agcagccgcg agttcatgcc attcctggac gaggtggtcg
     4681 cggtggaccc ggatcgcgtg agggtttggg ccgacgacga gcacggccgc ttcgccaccg
     4741 tggacgacct gctgtccggt gccggtgcga cgacggccgt ctacgtgtgc gggcccaccg
     4801 ccatgctcga agcggtgcgc gtggcccgcg acgaacacgc cgacgcgcca ttgcattacg
     4861 agcggttcag cccaccgccg gtcgtcgacg gaatcccttt cgagctggaa ctggcgcggt
     4921 cgcagcgggt gcttgccgtt ccggcgaacc ggtcggccct cgacatcatg ctcgatcggg
     4981 atccgggaac accctactcg tgccggcagg gcttttgcgg gacatgcaaa gtcaaagtgc
     5041 ttgcgggaca ggtagatcac cgggggcgca ccgccgtggg cgatgacgag atgctggtct
     5101 gtgtgtcg
//
LOCUS       DASSGQ010000202         8629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_166510,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000202 DASSGQ010000000
VERSION     DASSGQ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8629
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166510"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFI55_08955"
     CDS             <1..209
                     /locus_tag="VFI55_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET7074190.1"
                     /translation="DSVGLSTSARERNIAGRVLLGGQPGRLVGTEVVIVDDIVTTGAT
                     ARESVRALRAAGIRVAAVLALASA"
     gene            557..1294
                     /gene="raiA"
                     /locus_tag="VFI55_08960"
     CDS             557..1294
                     /gene="raiA"
                     /locus_tag="VFI55_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003901577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET7074191.1"
                     /translation="MSRLTVDSGQILEQPLAETNEPSEQITNVEVVFKGRNVEIPDHY
                     RVYVSEKLARLERFDRSIYLFDVELKHAPNRRQRKFCQRVEITARGRGPITRAEACAD
                     SFYAAFESAVGKLESRLRRGKDRRKVHYGDKTPVSLAAATAVVPPPEKAFNVQPAEPH
                     HHDPAQPEADVNVGGHEDDRDRNGHEPGRVVRTKEHQATPMSVDEALYEMELVGHNFF
                     LFYDKETERPSVVYRRHAYDYGLIRLS"
     gene            1410..4262
                     /gene="secA"
                     /locus_tag="VFI55_08965"
     CDS             1410..4262
                     /gene="secA"
                     /locus_tag="VFI55_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET7074192.1"
                     /translation="MLSKLLRLGEGRMVKRLKRVADYVSTLSDDVEKLTDAELRAKTD
                     EFKRRLADETNPETLDELLPEAFAVAREAAWRVLDQRPFDVQVMGAAALHLGNVAEMK
                     TGEGKTLTCVLPAYLNALAGKGVHIVTVNDYLAKRDSEWMGRVHRFLGLHVGVILATM
                     TPDERRVAYNADITYGTNNEFGFDYLRDNMAHSLDDLVQRGHNFAIVDEVDSILIDEA
                     RTPLIISGPADGASNWYTEFARLAPLMEKDVHYEVDLRKRTIGVHEKGVEFVEDQLGI
                     DNLYEAANSPLVSYLNNALKAKELFNRDKDYIVREGEVLIVDEFTGRVLIGRRYNEGM
                     HQAIEAKEHVEIKAENQTLATITLQNYFRLYDKLAGMTGTAQTEAAELHEIYKLGVVS
                     IPTNKPMVRTDQSDLIYKTEEAKYIAVVDDVSERYEKGQPVLIGTTSVERSEYLSRQF
                     QKRRIPHNVLNAKYHEQEAAIIAEAGRRGGITVATNMAGRGTDIVLGGNVDFLTDLRL
                     RARGLDPLETPDEYEQAWHAELPVVKEEAGKEATEVIEAGGLYVLGTERHESRRIDNQ
                     LRGRSGRQGDPGESRFYLSLSDELMRRFNGAALEAMLNRLNLPDDVPIENKMVTRAIK
                     SAQTQVEQQNFEVRKNVLKYDEVMNQQRMVIYAERRRILEGENLQQQAKDMLVDVITA
                     YVDGATNEGYAEDWDLDALWTALKTLYPVGIKPESLTSRDADSDRDELTREELLDALL
                     KDAERAYAAREAELEEIAGEGAMRQLERNVLLNVIDRKWREHLYEMDYLKEGIGLRAM
                     AQRDPLVEYQREGYDMFMAMLDGMKEESVGFLFNVTVEAVPAPQVAPLETPEGLAELG
                     TSPGAEPQQDADAAPAAVPEEHPSTLRAKGIDNEAPALTYSGPSEDGSAQLQRNGGDG
                     EKTPAGVAGGASRRERRAAARQQGRGAKPPKSVKRR"
     gene            4348..5898
                     /locus_tag="VFI55_08970"
     CDS             4348..5898
                     /locus_tag="VFI55_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET7074193.1"
                     /translation="MPFGGAVSRTEVLAALSLAIDLGLGQPMDHMLRSAAMGSRLAER
                     LGLSERERGTVFYTGLVMWIGCHADSHEYAQWFGDDIAVRHASHLIDWSGAPYRRFLM
                     SNLGRGAPLPQRARLAARVFLDARGNLGALVQSHCLSAALLAEEIGLGADVCEALPYA
                     YERWDGSGLPAGAAGTQIPVAMRVAQLADIADVHHRAYGASAAIAEVRRRSGKQFDPD
                     MVAAFTAGADDLLREDEDVWSTAAGLAPDPGEPLSGPALDRLLCAMGDFADLKCPFTL
                     GHSRAVAQLAENAGECAGLPRDDIDILRRAGYVHDLGRIGVSNRVWEKPGELTHAERE
                     RVHLHPYLTGRILARVGGLKAVREVAVNHHERLDGSGYPNGLRGDDLDARDRLLAAAE
                     SYCASMEPRPYRDARDGDAAAKRLRSEAARGRLDGDAVEAVLEAAGHRPSRRVARPAG
                     LTRREAEVLVHVAQGLSNREIASALWISEKTVRNHVEHIYAKLGVSNRIGASLYATRH
                     GLTGGAPN"
     gene            5977..6813
                     /locus_tag="VFI55_08975"
     CDS             5977..6813
                     /locus_tag="VFI55_08975"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7074194.1"
                     /translation="MTSTGRHETIPRNPAAGGGGSLPYEWQTGLSSGLWDKVLTDGHA
                     SLVRARHAFRYLPSAPRCKLCNNPFGGPVGRLFAAVGFRQSRKNPNLCIRCCDALPRG
                     GAEVDVAVLFADVRGSTALGQRRAAADFAALLNRFYSAATQTLLRHDAVIDKLIGDEV
                     MAFFVQGISGPRYRQRAVEAGVDLLKAVGYDTPGGPWLDVGVAVHAGVAYVGNVGGAV
                     VDFTALGRPVNAAARMQQLAAGGELLVAAGVADEMTADMQRRTLNLRGQDEPINVFVH
                     TF"
     gene            complement(6867..8582)
                     /locus_tag="VFI55_08980"
     CDS             complement(6867..8582)
                     /locus_tag="VFI55_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7074195.1"
                     /translation="MSELTCNAATIGLSEARRPGARVDAIHVGRRVGGRQILHKLSLS
                     VEPGELVAIAGGSGAGKTTLLEILAGLQPPSTGEVRQDGVVRRARVSADAKVGYVPQD
                     DIIHLEMPLRRTLRYAALLRLPAGTSAVEAERIVEETLGDLDLADRADVPVRALSGGQ
                     RKRASIAVELLTRPRVFFLDEPTSGLDPSTAADVMRLLRRLSARGVTVVLTTHEPAGI
                     DRCDRVVFLARDGHLAFTGSPAAARRYFGVENLSEVYERLASEHTPQIWADRFAESLP
                     TPTPRPGLTRPAASATRSGARGTGMVRQWWLLTRRNVDVLCRNRLTLAILLGSPVLVT
                     AMMATLFRRGAFDPGGASGVGPAQIVFWIAFDGFFFGLTYGLLQIVGELAVFRRERLA
                     GLSVGAYVASKVTALLPVLAVVSAVLLLVLRALGRLPAAGWDVYALLFATIVIEAVSA
                     LALGLFASAAVSNAAQAALALPMLCFPQVLFGGAIVPVDQMAAPGRLMSLVLSNRHAF
                     DALGRDLQLDRYTTTLPAMSVYRETFQGGNTVSLIALASFAVALTLATAWLLDRRSRP
                     GVSTR"
BASE COUNT         1580 a         2821 c         2947 g         1281 t
ORIGIN      
        1 gcgactcggt gggcctttcc acgtccgccc gcgagcgcaa catcgccggc cgggtgctgc
       61 tgggtggtca accaggaaga cttgtgggca ccgaggtcgt gatcgtcgac gacatcgtca
      121 ccaccggggc gacggcgcgc gagtcggtgc gggccctgcg cgccgccggg atccgggtgg
      181 ccgcggtgct ggccctcgcg tccgcctgaa tccgtccaga ccccgacacg ccgtgaaatc
      241 catgaaagcc cgatgaggcc gccaagaggt tgtgaagaac ttgcaacagc tcagcgaaat
      301 cggtggcgcg ccaaggcgaa cacgagctaa cgtcgaggac aaccttcagg aacatcctgt
      361 gttcctggtc tgactcactg gtcgcgtttg ccagggcctg acgccagttg cgaagggggt
      421 gagtattcga caccttgcac cggcgggcgc cttgcggcgg tgaccactct ggaaccgctc
      481 ccaccctgtc ggcgcgtctt gtaaaaagcc ggcacgctgc gcgtgcgacg tggaatagag
      541 aaacgagttg tcacgtatgt caaggcttac cgtggattcc ggtcagattc tggaacaacc
      601 gctcgcagaa accaacgagc cgtccgaaca gataaccaac gttgaagttg tgttcaaggg
      661 ccgcaacgtc gaaatccccg atcactaccg cgtctacgtc tcggaaaaac tcgcccgact
      721 cgaacgattc gatcgctcca tctacctttt cgacgtcgag ctcaaacacg cgccgaaccg
      781 gcgtcagcga aaattctgtc aacgcgtgga aataacggcc cggggtcgcg gaccgatcac
      841 ccgggcagag gcctgcgccg acagcttcta tgccgccttc gagtccgcgg tcggcaagct
      901 ggagagccgg ttgcggcgcg gcaaggatcg ccgcaaggtc cactacggcg acaagacccc
      961 ggtttccctg gccgccgcca ccgcggtggt cccgccgccg gagaaggcct tcaacgtcca
     1021 gccggccgag ccgcaccatc atgatccggc gcaaccggaa gccgacgtca acgtgggtgg
     1081 ccatgaggac gaccgggacc gcaacggcca cgagccgggg cgggtggtgc gcaccaaaga
     1141 gcaccaggcc acgccgatgt cggtcgacga ggcgctctac gagatggagc tggtcgggca
     1201 caatttcttc ttgttctacg acaaggagac cgaacggccg tccgtggttt accgccggca
     1261 cgcctacgac tacggcttga tcaggctttc ctgaccggca gtcgcctttc caaggtccgg
     1321 cagcacgacg ttcgccggct tcgcacgcgt gtcacctacg atggatggcg cctaccgcca
     1381 aagattgacg tccgacaggg gacatagctg tgctgtcgaa attgctgcgc ctgggtgaag
     1441 gtcgcatggt caagcgtctc aagcgggtcg ccgactatgt cagcaccttg tccgacgacg
     1501 tcgaaaagct caccgacgcc gagctgcggg ccaaaaccga cgagttcaag cggaggctgg
     1561 ccgacgagac aaacccagag actctcgacg aactgctccc cgaagcgttc gcagtggccc
     1621 gcgaggccgc ttggcgggtg cttgaccagc gtccgttcga cgtgcaggtg atgggcgcgg
     1681 ccgcgctgca cctgggcaat gtcgccgaga tgaagaccgg tgaaggcaag acgctgacct
     1741 gcgttcttcc cgcctacctc aacgcgctgg ccggcaaggg cgtacacatc gtcaccgtca
     1801 acgactacct ggccaaacgc gacagcgagt ggatgggccg ggtgcaccgc ttcctcgggc
     1861 tgcacgtcgg ggtgatcctg gcgacgatga cgcccgacga acgccgggtg gcctacaacg
     1921 ccgacatcac ctacggcacc aacaacgagt tcggcttcga ctacctgcgc gacaacatgg
     1981 cgcattcgct cgacgacctg gtgcaacgcg gacacaactt cgcgatcgtc gacgaggtgg
     2041 actccatcct catcgacgag gcccgcacgc cgctgatcat ctccggtccc gccgacggcg
     2101 cctccaactg gtacaccgag ttcgcacggc tggcgccgct gatggaaaag gacgtccact
     2161 acgaggtcga cctgcgcaaa cgcaccatcg gcgtgcacga gaagggcgtg gagttcgtcg
     2221 aagaccagct cggcatcgac aacctctacg aggccgccaa ctccccgctg gtcagctacc
     2281 tcaacaacgc cctgaaggcc aaggagctgt tcaaccgcga caaggactac atcgtccgcg
     2341 aaggcgaagt gctcatcgtc gacgagttca ccggccgggt gctgatcggg cgccgctaca
     2401 acgagggcat gcaccaggcc atcgaggcca aggagcacgt cgagatcaag gccgagaacc
     2461 agacgctggc gaccatcacg ctgcagaact atttccggct ctacgacaag ctcgccggca
     2521 tgaccggtac cgcgcagacc gaggccgccg agctgcacga gatctacaag ctgggcgtgg
     2581 tcagcatccc gaccaacaag ccgatggtcc gcaccgacca gtccgacctc atctacaaga
     2641 ccgaggaagc caagtacatc gcggtggtcg acgacgtgtc ggagcgctac gagaagggcc
     2701 agccggtgct gatcggcacc accagcgtgg agcgctccga gtacttgtcg cgacagttcc
     2761 agaaaaggcg gatcccgcac aacgtgctca acgccaagta ccacgagcag gaggcggcga
     2821 tcatcgccga ggcgggccgg cggggcggca tcacggtcgc gaccaacatg gccggccgcg
     2881 gtaccgacat cgtgctgggc ggcaacgtcg acttcctcac cgacctgcgg ctgcgtgccc
     2941 gcggactgga tccgctggaa acgcccgacg agtacgagca ggcctggcat gcggagctgc
     3001 ccgtcgtcaa ggaggaggcc ggcaaagagg ccaccgaggt gatcgaggcg ggtggcctgt
     3061 acgtgctggg caccgagcgg cacgagtcgc gccgcatcga caaccagctg cgtggccgct
     3121 ccggccggca gggcgacccc ggcgaatcgc gcttctattt gtcgctgagc gacgagctga
     3181 tgcgccgttt caacggtgcc gcgctagagg cgatgctcaa ccggctgaac ctgcccgacg
     3241 acgtgccgat cgaaaacaag atggtcaccc gcgccatcaa gagcgcccag acccaggtcg
     3301 agcagcagaa cttcgaggtc agaaagaacg tcctgaagta cgacgaggtg atgaaccagc
     3361 agcgcatggt gatctacgcc gaacgccgcc gcatcctcga gggcgagaac ctgcagcagc
     3421 aggccaagga catgctcgtc gacgtcatca ccgcgtacgt cgacggcgcg acgaacgaag
     3481 gctacgccga ggattgggat ctggacgcgt tgtggacggc gctcaagacg ctctacccgg
     3541 tcggcatcaa gcccgagagc ttgacgagtc gagatgccga ctccgaccgc gacgagctga
     3601 cccgcgaaga gttactcgac gccctgctca aagatgccga gcgagcttat gccgcaaggg
     3661 aagccgagct cgaggaaatc gccggcgagg gtgcgatgcg ccagctggag cgcaacgtgt
     3721 tgctcaatgt catcgaccgc aagtggcgcg agcatctcta cgagatggac tacctcaagg
     3781 aaggcatcgg gctgcgtgcg atggcgcagc gcgatccctt ggtggagtac cagcgcgagg
     3841 gctatgacat gttcatggcc atgctcgacg ggatgaagga agagtcggtc gggttcttgt
     3901 tcaatgtcac cgtggaggcg gtgcccgccc cgcaggtggc gccgctggag acgccggagg
     3961 gcctggcgga gttgggaacc tcgcccggcg ccgaaccaca acaagacgcg gacgctgcgc
     4021 ccgccgcggt gcccgaagag cacccaagta ccttgcgcgc caagggaatt gataacgaag
     4081 cgcccgccct gacctattcc ggcccgtcgg aggacggctc ggcacagctg cagcgcaatg
     4141 gcggtgacgg ggagaagacg cccgccggcg tggcgggtgg tgccagccgg cgcgaacggc
     4201 gtgctgccgc gcggcaacaa ggccgcggcg ccaagccgcc gaagtcggtc aagaggcgct
     4261 agcgggcccg gcgcttcgcg cctgccgttt cgatgcccgg ctacactgac gccgcggccg
     4321 tgacgggtcg agaaaggggc gagtttcatg cccttcggcg gagcggtgtc gcggactgag
     4381 gtcctggcgg cgctgtctct ggcaatcgac ctcgggctcg ggcagccgat ggatcacatg
     4441 ctgcggtcgg cggcgatggg ctcgaggctg gccgagcgac tcggactcag cgagcgtgag
     4501 cgtgggaccg ttttctatac gggcctggtg atgtggatcg ggtgtcatgc cgactcgcac
     4561 gagtatgcgc agtggttcgg cgacgacatc gcggtgcgac acgccagcca cctcatcgac
     4621 tggtcgggcg cgccgtaccg ccgatttctg atgagcaatc tgggtcgagg ggcgccgttg
     4681 ccgcaacggg cccgattggc ggccagagtg ttcctggacg cgcgcgggaa tctcggcgcg
     4741 ttggtgcagt cccattgtct gtcggccgca ctgctcgccg aggagatcgg cctgggcgcg
     4801 gatgtgtgcg aagccctgcc gtacgcgtac gaacgatggg acggcagcgg ccttcccgct
     4861 ggagccgccg ggacccagat accggtggcg atgcgggtgg ctcagcttgc cgacatcgcc
     4921 gatgtgcacc atcgcgccta cggcgcgagc gccgcgatcg cggaggttcg ccgacgcagc
     4981 ggcaaacagt tcgacccgga tatggtcgca gcgttcaccg cgggcgccga cgatctgttg
     5041 cgcgaggacg aagacgtatg gtcgaccgct gccggcttgg cgccggatcc gggcgaaccg
     5101 ctcagcggcc ccgcgctgga ccgactgctg tgcgcgatgg gtgacttcgc ggatctgaaa
     5161 tgcccgttca cccttggcca ttcacgtgct gtcgcgcagc tcgcagagaa cgccggggag
     5221 tgcgccgggc tgccgcgcga cgatatcgac attctgcgcc gcgcgggcta cgttcacgat
     5281 cttgggcgga ttggtgtgtc gaaccgggtg tgggaaaagc cgggcgaatt gacccacgcg
     5341 gagcgcgagc gcgtgcattt gcacccctat ctgacgggcc ggattctggc ccgggtcggt
     5401 ggactgaagg ccgtccggga ggtggcggtg aaccatcacg aacgcctgga cgggtcgggg
     5461 tatccgaacg gtctgcgggg cgatgacctg gatgcccgcg accggctgct cgctgctgcc
     5521 gaaagctatt gcgcctcaat ggagccacga ccgtaccgcg atgcccggga cggggacgcc
     5581 gcggccaaac gactgcgaag cgaagcggcc cgcgggcgcc tggacggcga cgcggtcgaa
     5641 gcagtgctgg aggccgcggg ccaccggccg tcgcgcagag tcgctcggcc ggcggggttg
     5701 actcggcgcg aggccgaggt attggtccat gtggcgcagg ggctatcgaa ccgggagatc
     5761 gcctcggcgc tatggatttc cgagaagacc gtgcgcaacc acgtcgagca catctatgcc
     5821 aagctcgggg tctcgaaccg gatcggggcc agcctgtacg cgacccggca cggcctgacc
     5881 ggcggtgcgc ccaactaaaa gcgcgtgccg ggccgacacg gatgtttggg gcggatgccc
     5941 catgtgcgtg cgggaaatcg aggcgaccat ttccgcatga ctagcactgg ccggcacgaa
     6001 acaatcccgc ggaatccggc cgcaggtggc ggcgggtccc tgccgtacga atggcaaacg
     6061 gggctctcct cgggactgtg ggacaaggtg ctcaccgacg gccacgcgtc cctggtcaga
     6121 gcgcggcacg cattccgcta tctgccctcg gcgccgcgct gcaagctctg caacaacccg
     6181 tttgggggcc cggtcggccg gctctttgcc gctgtcggat tccggcaatc ccgtaagaat
     6241 cccaacctgt gcatacgatg ctgcgatgca ctgccgcgag ggggcgcgga ggtcgacgtc
     6301 gcggtgctgt tcgccgatgt tcgcgggtcc acggcgttgg gccagcgccg ggccgccgcg
     6361 gacttcgccg cgttgctcaa ccggttttac agtgccgcca cgcagacact gctgcgccat
     6421 gacgccgtga tcgacaagct catcggtgat gaggtgatgg cgtttttcgt ccaaggcatc
     6481 agcggccctc ggtatcgtca gcgagccgtc gaggcgggag tcgacctgct caaagcggtg
     6541 gggtacgaca caccaggtgg accctggctg gacgtcggtg tcgcagtgca cgcgggcgtc
     6601 gcatacgtcg gcaacgtcgg cggggcggtc gtcgacttca ccgccctcgg tcgcccggtc
     6661 aacgccgcgg cccggatgca acaactcgcc gccggcggcg agttgttagt cgccgccggc
     6721 gtcgccgacg aaatgacggc cgacatgcag cggcgcacgc tgaacctgcg gggacaagac
     6781 gagccgatca acgtcttcgt ccacacattc tgagccttcg gtcaactcct gcacgccgag
     6841 aatcggccgc ggtcgcggcg cggccgtcat cgcgtcgata cgccgggccg ggatcgccgg
     6901 tccagtagcc aggcggttgc cagcgtgagg gcaacggcga acgaggcgag cgcgatgaga
     6961 ctgacggtgt tgcccccctg aaaggtctcc cggtagacgg acatggccgg cagcgtggtg
     7021 gtgtagcggt cgagttgcag gtcgcgcccc agcgcatcga aggcgtggcg gttggatagg
     7081 accagactca tcaggcgccc gggggcggcc atctggtcga ccggcacgat ggccccaccg
     7141 aacagcacct gagggaagca cagcatcggc agggcgaggg cggcctgcgc ggcgttggag
     7201 acggcggccg aggcgaacag gcccagggcc agcgccgaaa ccgcttcgat cacaatcgtc
     7261 gcgaacagca gggcgtacac gtcccatccc gcggcgggca gccgacccag cgcgcgcagc
     7321 accagcagca acaccgcgct cacgaccgcg agaaccggaa gcagcgccgt caccttggac
     7381 gccacgtagg cgccgacgct gagcccggcc aggcgttcgc gccggaagac ggccagctcg
     7441 ccgacgatct gcagcaggcc gtacgtcagg ccgaagaaga agccatcgaa ggcgatccag
     7501 aacacgatct gagccgggcc gacgccggac gcgccacccg gatcgaacgc accgcgcctg
     7561 aacagggtcg ccatcatcgc cgtcacgagc accggtgagc ccagcaggat cgccagcgtc
     7621 agccggttgc ggcagagcac gtcgacgttg cgccgtgtga gcagccacca ttgccggacc
     7681 atgcccgtgc ccctggcgcc ggaccgcgtg gccgaggcgg ccggtcgagt gaggcccggc
     7741 cggggtgttg gcgtcgggag gctttccgcg aaccggtcgg cccagatctg cggcgtgtgc
     7801 tctgaagcca ggcgctcgta gacctcgctg aggttttcca cgccgaagta gcgccgcgcc
     7861 gctgccgggc tgccggtgaa ggccaagtga ccgtcgcggg ccaggaagac cacccggtcg
     7921 caccggtcaa tgccggccgg ctcatgggtc gtgaggacga cggtgacccc acgcgcgctg
     7981 agccggcgca gcagtcgcat cacgtcagcc gcagtcgagg gatcgagccc ggaggtgggt
     8041 tcgtccagga agaaaacgcg tggcctggtc aggagctcca cggcgatgct ggcccgcttg
     8101 cgctggccgc cggacagtgc acgcaccggc acgtcggccc gatcggccaa gtccaggtcg
     8161 cccagggtct cctcgacgat ccgctccgct tcgaccgcgg atgtgcccgc cgggagcctt
     8221 agcagggccg cgtagcgcag cgtgcggcgc aggggcatct cgaggtggat gatgtcgtcc
     8281 tggggcacgt agccgacttt cgcgtcggcg ctgacgcggg cccgacgaac gacgccgtcc
     8341 tgccgaacct ccccggtgga cggcggttgc agtccggcga ggatctccag caaagtggtc
     8401 ttccccgcgc cgctgccgcc ggcgatggcg accaattcac cgggttccac tgagagcgac
     8461 agtttgtgca ggatctgccg gccaccgacg cgccggccca cgtggatcgc atcgacacgc
     8521 gcgcctggtc gccgcgcctc cgacaacccg atggttgctg cgttgcatgt caattcgctc
     8581 atgatctggt ctccatgcac tctcgaagtg gccctgtcac ggccgatca
//
LOCUS       DASSGQ010000203        10698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1138674,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000203 DASSGQ010000000
VERSION     DASSGQ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10698
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138674"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VFI55_08985"
     CDS             <1..1083
                     /locus_tag="VFI55_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PPE domain-containing protein"
                     /protein_id="HET7074196.1"
                     /translation="NLGNGNFGIANIGNANFGIGNTGNRLIGFGRPGNGNVGVLLINE
                     NQVGAGPGETALIMGGTGFNPLPRPDTVTFAEEFITPTHPNYTAYFLVTPEKLFPFTG
                     PTSLSLDASVAQGVANLNAAIMAQQAAGNHTVVFGVSQSAVVATVEMRYLETLPPGVR
                     PAPDQLSFVLAGNPDRPDGGLFARLPGLSLPGFTSYGATPTNAYPTIDYAFQYDGVAD
                     FPQYPIDILADANAIAGIFFVHPAYDTLTPSQITSGVVEPVSPGDTQTTYILIPNPDL
                     PLLDPLRAIPLVGNPLADLVQPDLRVLVELGYDRTAYQDVPTPFGLFPNVDPATVAAE
                     LQQGTVQGVYDALADVGLPPAVPPFP"
     gene            complement(1125..2168)
                     /locus_tag="VFI55_08990"
     CDS             complement(1125..2168)
                     /locus_tag="VFI55_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7074197.1"
                     /translation="MTWLLVAILVLVAATVGAWAYQTANRLDRLHVRYDLSWQALDGA
                     LARRAVVARAVAIDAYGGALEGRRLAALADAAEGAPRHGRENAENELSAALAVVDPVS
                     LPAGLIAELADAEARVLLARRFHNDAVRDTLALAERRLVRTFRLGGTAGLPSYFEIVE
                     RPHALAHGERGVLNHRTSARVVLLDATGAVLLLCGSDPAVTGREAPRWWFTVGGEVRQ
                     GERLAEAAARELAEETGLRVAPADMIGPVWRRDEVFEFNGSLIDSEEFYLVYRTRRFE
                     PSIAGRTELERRYIHGARWCDANDIAELTAAGEQVYPLQLGELLPTANLLADGAADTG
                     AGHAGAGVPQPIR"
     gene            complement(2168..3304)
                     /locus_tag="VFI55_08995"
     CDS             complement(2168..3304)
                     /locus_tag="VFI55_08995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7074198.1"
                     /translation="MRIGMICPYSFDVPGGVQSHVLQLAEVTRARGHEVSVLAPASPH
                     ASLPDYVVSAGRAVPIPYNGSVARLRFGPATHRKVKKWLVEGDFDVLHLHEPNAPSLS
                     MLALNIAEGPIVATFHTSTTKSLTLTVFQGILRPMHEKIVGRIAVSDLARRWQMEALG
                     TDAVEIPNGVDVESFATAQPLPGYPRPGRTVLFLGRYDEPRKGMSVLLDALPRVVERF
                     ADVQLLIVGRGDEDELRRQAGESVKHMRFLGQVDDAEKASAMRSADVYCAPNTGGESF
                     GIVLVEAMAAGTAVVASDLDAFRRVLRDGECGRLVPVDRPELQGAALADALIEVLEDD
                     ALRQRYVAAGSAAVHRYDWSVVASQIVRVYETVAGSGAKVQVAS"
     gene            complement(3304..4233)
                     /locus_tag="VFI55_09000"
     CDS             complement(3304..4233)
                     /locus_tag="VFI55_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HET7074199.1"
                     /translation="MSRLVTRNAVDWAYATGWMAVRVMPESAARTAFDSGARYAARRG
                     GPDQLRKNLARVIGVAPADVPDALMRASLASYGRYWREAFRLPTMNLPALARRLHDCV
                     LGQDHLDAALAAGRGAVVALPHSGNWDMAGVWLAHTHGTFTTVAERLKPESLYRRFIR
                     YRESLGFEVLPLSGGRRPPFEVLCERLRENRVVCLMAERDLTRTGVQVDFFGEPTRMP
                     AGPAKLAIATGAALLPVHCWFESHQVQGWGQSIHPALDCASGDVTAITQVLADQFAKN
                     IAAHPEDWHMLQPQWLADLSTERRAKLAGRGDT"
     gene            complement(4281..4976)
                     /locus_tag="VFI55_09005"
     CDS             complement(4281..4976)
                     /locus_tag="VFI55_09005"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET7074200.1"
                     /translation="MSKVPFLSRAAFARLTTPTARACLRVGLTPDAVTIFGTIVAVAG
                     ALTLFPMGKLFAGALVVAFFVLFDMLDGAMARERGGGTRFGAVLDATCDRVSDGAVFC
                     GLLWWVAFGLRDRPLVVATLICLVTSQVISYIKARAEASGLRGDGGIIERPERLIIVL
                     IGAWLSDLPVYPLPWALPVAMWLLAAASLVTCAQRLHTVRTSPGAGDPMVPLPPAGDR
                     AKPGPEHRGTGEP"
     gene            complement(4973..5566)
                     /locus_tag="VFI55_09010"
     CDS             complement(4973..5566)
                     /locus_tag="VFI55_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET7074201.1"
                     /translation="MSDRDRAEERAGRGEDTIRDTGVGEQDHLQRLWTPYRMTYLAEA
                     PVKRDNNSSGKPEHPFTYIPHLSDEDGLVVARGELVYAVLNLYPYNPGHLMVVPYRRV
                     SELEDLTDAESAELMAFTQKAIRVIKNVSRPHGFNVGMNLGTSAGGSLAEHLHVHVVP
                     RWGGDANFITIIGGSKVIPQLLRETRQLLATEWAKSS"
     gene            complement(5569..7623)
                     /gene="thrS"
                     /locus_tag="VFI55_09015"
     CDS             complement(5569..7623)
                     /gene="thrS"
                     /locus_tag="VFI55_09015"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET7074202.1"
                     /translation="MSVAAQPAPAAPIRVPAGTTAAAAVGEAGLPRRGTPDAIVVVRD
                     ADGKLRDLSWVPDADADVVPVAANTDEGRSVIRHSAAHVLAQAVQDLFPQAKLGIGPP
                     ITDGFYYDFDVAEPFTPEDLAALEKRMRQIVKDGQLFDRRVYESKDQARRELADEPYK
                     LELVDDKSGDPDVMEVGGDELTAYDNLNPRTRERVWGDLCRGPHIPTTKHIPAFTLTR
                     SSAAYWRGDQKNASLQRIYGTAWESPQALDHHLEMLAEAQRRDHRKLGVELDLFSFPD
                     EIGSGLAVFHPRGGVVRSELEDYSRRKHAQAGYEFVNTPHITKEQLYITSGHLQWYAD
                     GMFPPMHLDAEYNEDGTVRKPGQDYYLKPMNCPMHHLIYRSRGRSYRELPLRLFEFGT
                     VYRYEKSGVVHGLTRVRGMTQDDAHIYCTSEQMRDELASLLRFVLDLLADYGLDDFYL
                     ELSTKDPDKYVGSDEVWDEATEVLREVAEASGLQLVPDPGGAAFYGPKISVQVKDALG
                     RSWQMSTIQLDFNMPERFDLEYTASDGTRRRPVLIHRALFGSIERFFGILTEHYAGAF
                     PAWLAPVQVVGIPVADEHVAYLKDVAAQLKSHGVRVEVDASDDRMAKKIVHHTSQKVP
                     FMLLAGDRDVEAGAVSFRFGDRTQINGVARDSAVDTIVKWIADRENAAPTAGLVKVAS
                     GE"
     gene            complement(7742..8365)
                     /locus_tag="VFI55_09020"
     CDS             complement(7742..8365)
                     /locus_tag="VFI55_09020"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET7074203.1"
                     /translation="MVGELQEHPGGGFNPPEPTTKGGPDYGRFIDAVRTLQDHARSVD
                     APDEVITEAAELLEKVSLFLSPFNADEWESPSGRRMDLPMRGNILTVPMSARRGPDGR
                     IRGWARFARFHLGRNGAVHGGALGMLFDTVLGLTSSIFTGGPYQRTAYLKVDYRQIVP
                     IEKELQFDAGVDRVEGRKIFVSGRLHDGDTLLTEADALFVWLKSDQL"
     gene            complement(8442..9326)
                     /locus_tag="VFI55_09025"
     CDS             complement(8442..9326)
                     /locus_tag="VFI55_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007172036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7074204.1"
                     /translation="MTAHNSHGAAQASGTFTLGGDLTVNRLGFGAMRLTGKGVWGPPA
                     DRDECVRVLRRAVELGVNFIDTADSYGPYVSEDIIREALHPYDGLVIATKAGLLRTGP
                     DVWIPLGNPSYLRQECEMSLRRLGVETIDLFQLHRIDRDFPLADQVGELVALKNEGKI
                     RHIGLSEIDVDQLSAAEEITQIVSVQNMYNLASRGAEPLLDAATSRGIGFIPWFPLAA
                     GPLAAADGPLQRIASEHDATPSQLALAWLLKRSPVMLPIPGTSRVAHLEENVAAAEIT
                     LTDDEFDTLAEAGAQRTV"
     gene            9375..9875
                     /locus_tag="VFI55_09030"
     CDS             9375..9875
                     /locus_tag="VFI55_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 domain-containing protein"
                     /protein_id="HET7074205.1"
                     /translation="MDLAAFEELPLTYPEIGATASGELPAGYDHLRVEDQIGTGRQRF
                     EQAADAVMRWGMQRGAGLRVQASSEVVQVSTVVVVRMGFLPAPCRVVYVIDEPDIRGF
                     AYGTLPGHPESGEERFVVRRDPATSAVFAEVSAFSRPATWWSKAGGPLVSVAQRVIAK
                     RYLRGV"
     gene            complement(9927..10271)
                     /locus_tag="VFI55_09035"
     CDS             complement(9927..10271)
                     /locus_tag="VFI55_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7074206.1"
                     /translation="MESISLTSLASDQLAEAERSHSGRAAHTIHGGHTHELRQTVLAL
                     LAGRDLSEHDSPGEATLQVLQGHVRLTAGADVWDGKTGDYLAIPAERHALHAVEDSVI
                     MLTVLKSLPSGQ"
     gene            complement(10432..>10698)
                     /locus_tag="VFI55_09040"
     CDS             complement(10432..>10698)
                     /locus_tag="VFI55_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7074207.1"
                     /translation="PPFGAHNADIDRVAADQGYTSITMWSGDVGDSAPENESSLVTRA
                     SKSFQPGQIVLMHANLPTITHCYGQLLDLIHSRNLQTATLREVF"
BASE COUNT         1683 a         3752 c         3551 g         1712 t
ORIGIN      
        1 aacctgggca atgggaattt cgggatcgcc aacatcggca acgcgaactt cggcatcggg
       61 aacaccggca acagacttat cggcttcggg cgccccggta acggcaacgt gggcgtgctg
      121 ctcatcaacg aaaaccaggt aggtgccggc cccggggaaa ccgcgttgat catgggcggc
      181 accggattca acccgctacc tcggcccgac acggtgacgt tcgccgagga gttcatcacc
      241 ccgactcatc ccaactacac cgcgtacttc ctggtgacgc ccgagaagct gtttcccttc
      301 accgggccga ccagcctgag cctcgacgcg tccgtggccc agggtgtggc gaacctgaac
      361 gccgcgatca tggcgcagca ggctgcggga aaccacaccg tcgtcttcgg cgtctcgcaa
      421 agcgccgtgg tggccaccgt ggaaatgcgc tatctggaaa ccctgccgcc cggcgtgcgg
      481 ccggcaccgg accagctgtc cttcgtgctg gccggtaatc cggaccggcc cgacggtggg
      541 ttgttcgcgc ggctccccgg tttgtcccta ccgggtttca cgagttatgg cgcaactccc
      601 accaacgcct accccaccat cgattacgcc ttccaatacg acggcgtcgc cgacttcccg
      661 cagtacccga tcgatatctt ggccgacgcc aacgccatcg ccggcatctt tttcgtgcac
      721 cccgcgtacg acaccctgac gccctcccag atcacctccg gtgtggtcga gccggtgtct
      781 ccgggagaca cccagaccac ctacatcctg attcccaacc cggatctgcc gctgctggac
      841 ccgctgcgtg ccatcccgtt ggtgggaaac ccactcgccg acctggtcca gccggacctg
      901 cgggtgctcg tcgagctggg ctacgaccgc accgcctatc aggatgtgcc cacgccgttc
      961 gggctgttcc ccaacgtcga tccggcgacc gtcgccgccg agctgcagca gggcacggtg
     1021 caaggcgtct acgacgcact ggccgatgtg ggcttgccgc ccgcggtgcc gccgttcccc
     1081 tgatttccct ggggctcccg ggacgcgacc cggcgccgcg tgcgtcagcg gatgggctga
     1141 ggcacgccgg ctccggcgtg acccgccccg gtgtcggcgg caccgtccgc cagcaggttg
     1201 gccgtcggca acaactcgcc cagttgcagc ggatatacct gctcgccggc cgcggtcagc
     1261 tcggcgatgt cgttcgcgtc gcaccaacgg gcaccgtgaa tgtaacggcg ttccaactcc
     1321 gttcgtcccg cgatggacgg ctcgaaccgg cgggtgcggt acaccaggta gaactcctcg
     1381 ctgtcgatca gcgagccgtt gaattcgaag acctcgtcgc gccgccagac gggcccgatc
     1441 atgtcggccg gcgcgacccg caggcccgtc tcttcggcca gctcccgcgc ggcggcctcg
     1501 gccaaccgct cgccttgccg cacttcgccg cccacggtga accaccaccg gggcgcctcg
     1561 cggccggtga ccgccggatc cgacccgcac agcaacagca ccgcgccggt cgcgtcgagc
     1621 agcaccaccc gcgccgaggt gcggtggttg agcacaccgc gttcgccgtg cgccaacgcg
     1681 tgggggcgct cgacgatctc gaaatagctt ggcaggcccg cggttccgcc gagccgaaag
     1741 gtgcggacca ggcgtcgttc ggccagcgcg agcgtgtccc gcacggcgtc gttgtggaat
     1801 cggcgggcca gcagcacccg cgcctcggcg tcggccagct cggcgatcag gcccgccggc
     1861 agcgacacgg gatcgaccac cgccagcgcc gccgagagct cgttttccgc gttctcacgc
     1921 ccgtgccgcg gcgcgccctc cgcggcgtcg gccagcgccg ccagtcgtct gccctccaac
     1981 gcgccgccat aggcatcgat cgcgacggcg cgcgccacca ccgcgcgccg cgccagcgca
     2041 ccgtcgagcg cctgccacga caggtcgtag cggacgtgca ggcggtccag ccggttggcc
     2101 gtctgatagg cccaggcgcc caccgtcgcc gccaccagca ccaagatcgc gacaagcagc
     2161 cacgtcatca gctggccacc tgaaccttgg cgccggaccc ggcgaccgtc tcgtataccc
     2221 gcacgatctg actcgccacc accgaccagt cgtagcggtg caccgccgcc gagccggccg
     2281 cgacgtagcg ttgccgcagc gcgtcatcct cgagcacctc gatgagggcg tcggccagcg
     2341 cggcgccttg caactccggg cggtcgaccg gcaccagccg gccgcactcg ccgtcgcgca
     2401 acacgcgccg gaaggcgtcc aggtcgctgg ccaccaccgc ggtcccggcg gccatcgcct
     2461 cgaccaggac gatgccgaag ctctcccccc cggtattggg cgcgcagtag acgtcggcgc
     2521 tgcgcatcgc cgacgccttc tcggcgtcgt ccacctggcc gaggaaacgc atgtgcttca
     2581 ccgattcgcc tgcctggcgg cgtaattcgt cctcgtcgcc gcggcccacg atcagcagct
     2641 gcacatcggc gaagcgctca accacccgcg gcagcgcgtc gagcagcacc gacatgccct
     2701 tgcgcggctc gtcgtagcgg cccaggaaca gcaccgtcct gcccggccgc ggatatcccg
     2761 gcagcggttg cgccgtggcg aacgactcga cgtcgacccc gttggggatc tccacggcgt
     2821 cggttcccaa cgcctccatc tgccagcgcc gggccaggtc ggacaccgcg atccggccga
     2881 cgatcttctc gtgcatcggc cgcaggatgc cctggaagac ggtcagtgtc agcgatttgg
     2941 tggtcgacgt gtgaaacgta gccacgatcg gcccctcggc gatgttcagg gccagcatcg
     3001 acaggctcgg cgcgttgggc tcgtgcaggt gcagcacatc aaagtcgccc tccacgagcc
     3061 actttttgac cttgcggtgg gtggccgggc cgaaccgcag ccgggccacc gacccgttgt
     3121 aggggatggg gacggcccgg cccgcggaga cgacgtagtc gggcagcgag gcgtgcgggg
     3181 aagccggcgc gagcacgctc acctcgtgcc cccgggcgcg cgtcacctcg gccagttgca
     3241 gcacgtgcga ctgcaccccg cccggcacgt cgaacgagta cggacagatc attccgatcc
     3301 gcatcaggtg tccccgcgcc cggcaagttt ggcccgcctt tcggtggaca ggtcggccag
     3361 ccactgcggc tgcagcatgt gccagtcctc gggatgggcg gcgatgttct tggcgaactg
     3421 atcggccagc acctgcgtga tggcggttac gtcgccgctg gcgcaatcga gggccgggtg
     3481 tatcgactgg ccccagcctt gaacctgatg ggactcgaac cagcagtgca ccggcaaaag
     3541 ggccgccccg gtcgcgatcg ccagcttcgc cggtccggcc ggcatccggg tgggttcgcc
     3601 gaagaagtcg acctggacac cggtgcgggt cagatcgcgc tccgccatca ggcacaccac
     3661 ccggttctcc cgcagccgtt cgcacaacac ctcgaagggc ggccgccggc cgccggacag
     3721 cggcagcacc tcgaagccga ggctttcgcg gtaccggatg aaacgccggt acagcgactc
     3781 gggtttgagg cgttccgcga cggtggtgaa ggtgccgtgg gtgtgcgcca gccacacccc
     3841 ggccatatcc cagttgccgc tgtgcggcaa ggccaccacc gccccgcgcc ccgcggccag
     3901 cgccgcatcc agatggtctt gcccgaggac gcaatcgtgg agccggcggg ccagcgcggg
     3961 gaggttcatc gtcggcagcc ggaaggcctc ccgccaatag cgcccgtagg aggccagcga
     4021 ggctcgcatc aacgcgtccg gcacgtcggc cggcgccaca ccgatgacgc gggccaggtt
     4081 cttacgcaac tggtccgggc cgccgcggcg ggccgcgtag cgggccccgc tgtcgaatgc
     4141 ggtgcgcgca gcggattccg gcatcacacg caccgccatc cagccggtcg cgtacgccca
     4201 gtcgaccgcg ttccgggtca ccaggcggct catcgggtgg cgtggcagcc caatcgtgtg
     4261 caggcccggg cgtgtgggga tcacggctcg cccgttcccc ggtgttccgg accgggcttg
     4321 gcgcggtcac cggccggcgg caacggaacc atgggatcgc cggccccggg cgaagtccgc
     4381 accgtgtgca gccgctgtgc gcaggtgacc aggctggccg ccgccaacag ccacatcgcc
     4441 accggcagtg cccacggcaa cgggtacacc ggaagatccg acagccaggc accgatcagc
     4501 acgatgatca gccgttccgg ccgttcgatg atgccgccgt cgccgcgcag cccgctcgcc
     4561 tcggcccggg ctttgatgta ggaaatcacc tgcgaggtca ccaggcagat cagggtcgcg
     4621 accaccagcg gccggtcccg caggccgaac gcgacccacc acagcagccc gcagaacacc
     4681 gccccgtcgc tgacgcggtc gcaggtggcg tccagcaccg cgccgaagcg ggtgccgccg
     4741 ccccgttccc gggccatcgc gccgtccagc atgtcgaaga gcacgaagaa cgcgaccacc
     4801 agtgcgccgg cgaacagctt gcccatcggg aacagcgtca gggcgcccgc cacggcaacg
     4861 atggtgccga aaatggtgac ggcatccggg gtcaagccca cccggagaca tgccctggcg
     4921 gtcggagtgg tgagccgcgc gaacgccgcc cgggacagga acggcacttt gctcatgagc
     4981 tcttcgccca ctcggtggcc agcagctgac gagtttcgcg cagcaactgc gggatcactt
     5041 tcgacccgcc gatgatggtg atgaagttcg cgtcgccacc ccagcgcggc acgacgtgga
     5101 cgtgcaaatg ctcggccaat gatccgcccg ccgacgttcc caggttcatc ccgacgttga
     5161 agccgtgcgg tcgcgacacg ttcttgatga cgcgaattgc cttctgggtg aaggccatca
     5221 actccgcgct ctccgcatcg gtcaggtcct cgagctcgga aacccgccgg tagggcacca
     5281 ccatcaggtg cccggggttg taggggtaga ggttgagcac ggcgtagacg agttcgccgc
     5341 gggcgaccac caggccgtct tcgtcggaca ggtgcggaat gtaggtgaac gggtgctcgg
     5401 gtttgcccga ggagttgttg tcacgcttca ccggcgcttc ggccaggtac gtcatccggt
     5461 acggcgtcca cagtcgctgc aggtgatctt gctcgccgac gcccgtgtcg cgaatggtgt
     5521 cctcaccgcg gccggcccgc tcctcggccc gatcgcgatc actcacggtc actcaccact
     5581 tgccactttc accagtccgg ctgtgggagc ggcgttttcg cgatcggcaa tccacttgac
     5641 gatggtgtcg accgcgctgt cgcgggccac gccgttgatc tgcgtgcggt caccgaagcg
     5701 gaagctcacc gccccggcct ccacgtcgcg gtcaccggcc agcagcatga acggcacctt
     5761 ctggctggtg tgatgcacga tcttcttggc catccgatcg tcgctggcgt ccacctcgac
     5821 ccgcactccg tgcgacttca gctgcgcggc aacatctttc aggtaggcca cgtgctcgtc
     5881 ggcgaccggg atgccgacca cctgaacggg cgccagccac gccgggaacg cccctgcgta
     5941 gtgctcggtg aggatgccga agaaccgctc gatcgacccg aacagcgccc ggtgaatgag
     6001 gacgggtcgc cggcgcgtcc catcagaggc ggtgtactcc aggtcgaacc gctcgggcat
     6061 gttgaagtcc agctggatgg tcgacatctg ccaactgcgg cccaacgcgt ccttgacctg
     6121 gaccgagatc ttcgggccgt agaacgccgc gccgcccggg tcgggcacca gctgtagacc
     6181 ggaggcttcg gcgacctcac gcagcacctc ggtcgcctcg tcccacacct catcggagcc
     6241 cacatacttg tccgggtcct tggtggacaa ctccagataa aagtcgtcca ggccgtagtc
     6301 ggccagcagg tccagcacga agcgcagcaa cgaggcaagc tcgtcgcgca tctgctccga
     6361 cgtgcagtag atgtgggcat cgtcctgcgt catgccgcgc actcgcgtca gcccgtgcac
     6421 cacaccggat ttctcgtagc ggtagacggt gccgaactcg aataggcgca acggaagttc
     6481 gcggtacgag cgccccctgg accggtagat cagatggtgc atcgggcagt tcatcggctt
     6541 gaggtagtaa tcctgcccgg gcttgcgcac cgtgccgtcc tcgttgtact ccgcgtcgag
     6601 atgcatgggc gggaacatgc cgtcggcgta ccactgcagg tggcccgacg tgatgtacag
     6661 ctgctccttg gtgatgtgcg gggtgttgac gaactcgtag cccgcctggg cgtgcttgcg
     6721 ccgcgagtag tcctccagct cgctgcgcac gacgccgccg cggggatgga aaaccgccag
     6781 accggaaccg atttcgtcgg ggaagctgaa caggtccagc tcgacgccga gcttgcggtg
     6841 gtcgcggcgc tgggcttccg cgagcatctc caggtgatga tcgagcgcct gcggtgactc
     6901 ccacgcggtc ccgtagatgc gctgcaggct ggcgtttttc tgatcgcccc gccaataggc
     6961 ggcggagctt ctggtcagcg tgaacgccgg gatgtgcttg gtggtcggga tgtgcggtcc
     7021 ccggcacagg tcgccccaga cccgttcgcg ggtgcgggga ttgaggttgt cgtacgcggt
     7081 gagctcgtcg ccaccgacct ccatcacgtc ggggtcgccg gacttgtcgt cgacgagttc
     7141 gagcttgtaa ggctcgtcgg ccagctcgcg gcgggcctgg tccttggact cgtagacgcg
     7201 ccggtcgaac agctgtccgt ccttgacgat ctggcgcatc cgcttttcca gcgccgccag
     7261 gtcctccggc gtgaacggct cggcgacgtc gaagtcgtag tagaagccgt cggtgatggg
     7321 cggcccgatg cccagtttgg cctgcgggaa gaggtcctgg acggcctgcg ccaacacgtg
     7381 cgccgccgaa tgcctgatga cgctgcgacc ctcgtcggtg ttcgccgcca ccgggacgac
     7441 gtcggcgtcg gcgtcgggca cccaactgag gtcgcgcagc ttgccgtcgg cgtcgcgcac
     7501 caccacgatc gcgtcgggcg tgccccgcct cggcaacccc gcctccccga cggcggctgc
     7561 cgcggtagtc ccggcaggaa cccggatcgg ggctgctggg gcgggctgtg cggcgacgct
     7621 catcgggttg ggtctccaat gtcagtttga ctgaatacgc tgattaaccg atgccaatgc
     7681 tatcggggtc gtccttgccg ggcccaagcc attagcggcg cgaaacctta cccgaccgcc
     7741 ttcacagctg gtccgacttg agccacacaa acaacgcgtc ggcctcggtc agcagtgtgt
     7801 cgccgtcgtg cagtcgcccc gacacgaaga tcttgcgccc ctcgacccgg tctacccccg
     7861 catcgaactg caattccttc tcgatcggca cgatctggcg gtaatcgacc ttgaggtaag
     7921 cggtgcgctg atagggaccc ccggtgaata tcgacgacgt caacccgagc acggtgtcga
     7981 agagcatgcc cagggctccg ccgtgcacgg cgccgttacg ccccagatga aaacgggcga
     8041 atcgggccca gccgcgaatc cggccgtccg gacccctgcg cgccgacatg ggaaccgtga
     8101 ggatgttgcc ccgcatgggc agatccatcc ggcgcccaga cggcgactcc cactcgtcgg
     8161 cgttgaacgg gctgagaaac agcgacacct tctccagcag ctccgcggcc tcggtgatca
     8221 cctcgtcggg cgcgtcgact gaacgcgcat ggtcctgcag cgtgcgcacg gcgtcgatga
     8281 acctgccgta atccggcccg cccttggtcg tcggttcggg tgggttgaag ccgccccccg
     8341 ggtgttcctg aagttctccc accagaccac cgtattgccc cgcaccccgt gtggcacagc
     8401 cacagacggg atcgcttggt gagagtggcc gttcgcggcg gttagaccgt ccgttgagca
     8461 ccggcttccg ccagggtgtc gaactcgtcg tcggtcagcg tgatctcagc ggcggcgacg
     8521 ttctcttcca ggtgcgcgac tcgggacgtg ccggggatcg gcaacatcac cggcgagcgt
     8581 ttcagcaacc acgccagcgc cagctgcgac ggtgtggcgt cgtgttcgga ggcgatgcgc
     8641 tgcaacgggc cgtccgcggc ggccagcggc ccggcggcca gcgggaacca cggaatgaac
     8701 ccgatgcccc ggctggtcgc ggcgtccaac agcggttcgg ccccccgaga cgccaggttg
     8761 tacatgttct gcaccgagac gatctgcgtg atctcttcgg ccgcactgag ctgatcgacg
     8821 tcgatctcgg acaggccgat gtggcggatc ttgccctcgt tcttcagcgc gaccagctcg
     8881 cccacctggt cggccagcgg gaagtcgcgg tcgatgcggt gcagctggaa caggtcgatg
     8941 gtctcgacgc cgaggcggcg caggctcatc tcgcattcct ggcgcaggta gctcgggtta
     9001 cccagcggga tccagacgtc cgggcccgtg cgcagcagcc ccgccttggt cgcgatcacc
     9061 aatccgtcat aggggtgcag cgcctcgcga atgatgtcct cggagacgta ggggccgtag
     9121 gagtctgcgg tgtcgatgaa gttgacgccg agttcgacgg cccgccgcaa cacccgaacg
     9181 cattcgtcgc ggtcggcggg aggaccccac acgcccttgc cggtcaggcg catggcgccg
     9241 aaaccgagcc ggttgacggt caagtcgccg ccgagggtga atgtccccga tgcctgggca
     9301 gcaccatgcg agttgtgcgc ggtcacgtca ccgaccgtac gccgccgggt gtgttgacgt
     9361 ggcaagctga ttaggtggac ctggcagcgt tcgaggagct tccgctgacc tatccggaga
     9421 tcggggcaac cgcgtcgggg gagctgcccg ccgggtacga ccacctgcgc gtggaggatc
     9481 aaatcggcac gggccggcag cgtttcgagc aagccgccga cgccgtgatg cgctggggca
     9541 tgcagcgcgg agccggcctg cgggtgcagg ccagctccga ggtcgtccaa gtctccacgg
     9601 tggtggtggt gcgcatgggt ttcctgccgg cgccgtgccg cgtggtgtac gtcatcgacg
     9661 aacccgacat ccgcggattc gcgtatggca ccctgccggg ccatccggag tccggcgagg
     9721 aacgcttcgt cgtgcgccgc gacccggcca cctccgcggt cttcgccgag gtgtcggcgt
     9781 tctcccggcc cgcgacctgg tggagcaagg ccggcggccc gctggtgtcg gtggcccaac
     9841 gcgtcatcgc caagcgctac ctgcgcgggg tgtgacgacc cggcgccgcg acgaccccca
     9901 gccggtcagg gagtgggaga ttccggctac tgacccgagg ggaggctttt cagcacggtc
     9961 agcatgatca ctgaatcctc gaccgcgtgc agggcatgcc gttccgccgg gatcgcgagg
    10021 tagtcaccgg tcttgccgtc ccagacgtcg gcgccggcgg tcaggcgcac gtgaccctgc
    10081 agcacctgca gcgtggcctc accggggctg tcgtgttcgg agaggtcacg gccggcgagc
    10141 aacgccagca cggtctgccg aagctcgtgg gtgtgacccc cgtggatggt gtgtgcggcc
    10201 cgtccgctgt gcgaacgttc cgcctcggcc agctggtcgg aggcgaggct ggtcaacgag
    10261 atggattcca taatcggccc cttcgatgtt tcaagcccgt attcagacgc tcaatagcag
    10321 tattcccgct gcgagcaagg ccgccgggtg gatcgaatcc ggccggcagc gcagcgcccc
    10381 tcgaattctg gccaacacca acgacttaca gtgcgccgag gccccgcttc gtcaaaaaac
    10441 ctctcgcagg gtggcggtct gcaggttgcg gctgtggatg aggtcgagca gttgtccgta
    10501 gcagtgcgtg atggtgggca ggttggcgtg catgagcacg atctggcccg gctgaaacga
    10561 cttggacgca cgggtcacga ggctgctttc gttctcgggt gccgagtcac cgacgtcccc
    10621 gctccacatg gtgatcgagg tgtacccctg atcggcggcc acccggtcga tgtcggcgtt
    10681 gtgggccccg aagggcgg
//
LOCUS       DASSGQ010000204         2667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1229708,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000204 DASSGQ010000000
VERSION     DASSGQ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229708"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2667
                     /gene="alaS"
                     /locus_tag="VFI55_09045"
     CDS             <1..>2667
                     /gene="alaS"
                     /locus_tag="VFI55_09045"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET7074208.1"
                     /translation="DHFVKAGHTEVPSASVILDDPNLLFVNAGMVQFVPFFLGQRTPP
                     YATATSIQKCIRTPDIDEVGITTRHNTFFQMAGNFSFGDYFKRGAIALAWDLLTNNIT
                     EGGYGLDPEKLWATVYLDDDEAAGLWQEIAGLPPERIQRRGMADNYWSMGIPGPCGPS
                     SEIYYDRGPEYGPEGGPVVNEDRYLEVWNLVFMQNERGEGTNKEDYEILGPLPRKNID
                     TGMGVERIALVLQGVHNVYETDLLRPIIDVVAARAPRPYDAGNHADDVRYRIIADHSR
                     TAAILIGDGVSPGNDGRGYVLRRLLRRVIRSAKLLGIEDPIVGDLMATVRDAMGPSYP
                     ELVTDFDRIRRIAVAEETAFNRTLASGSKLFEEVAGATKADGATVVSGSDAFTLHDTY
                     GFPIELTLEMAAEAGLQVDESGFRELMAEQRKRAKDDAAARKHAHADLSAYRELVDAG
                     PTEFTGFDELTSESRILGIFVDGKRVPVVAHGTDGSDRVELVLDRTPLYAESGGQIAD
                     EGTISGTGANGSARAAVTDVQKIAKTLWVHRVNVESGEFVEGDTVIAAVDAGWRRGAT
                     QGHSGTHMVHAALRQVLGPNAVQAGSLNRPGYLRFDFNWQGPLTEDQRTEIEEVTNQA
                     VQADFEVHTFLEKLDTAKAMGAMALFGEAYPDEVRVVEIGGPFSLELCGGTHVHNSAQ
                     IGPVTILGESSIGSGIRRVEAYVGLDSFRHLAKERALMAGLASSLKVPSDEVPARVAN
                     LVERLRAAEKELERARLASARVAAANAAAGAERIGDVRVVVQRVPGEMTAGDLRSLVG
                     DIRGKLGSDPVVVALISVNSGDEGTTVPYAVAANAAAQDLGFRANDLVKLLAAAVDGR
                     GGGKADLAQGSGKDPTGIDAALDAVRAQISEIA"
BASE COUNT          445 a          899 c          932 g          391 t
ORIGIN      
        1 gatcacttcg tgaaggcggg tcacaccgag gtgccgagcg catcggtgat cctcgacgac
       61 ccgaatctgc tgttcgtcaa cgccggcatg gtccagttcg tgccgttctt cctgggtcag
      121 cgcacgccgc cgtacgccac ggccaccagc atccagaagt gcatccgcac ccccgacatc
      181 gacgaggtcg gcatcaccac ccggcacaac accttcttcc agatggccgg caatttttcg
      241 ttcggcgact atttcaagcg gggggccatc gcgctggcct gggatctgtt gacgaataac
      301 atcaccgaag gtggatacgg gctggacccg gaaaagctct gggccacggt gtatctggac
      361 gacgacgagg ccgcggggct gtggcaggag atcgccgggc tgccgcccga gcggatccag
      421 cgccgcggca tggccgacaa ctactggtcg atgggcatcc ccggtccgtg cgggccgtcg
      481 tcggagatct actacgaccg gggacccgag tacggcccgg agggcggccc cgtcgtcaac
      541 gaggaccgct acctcgaggt ctggaacctc gtgttcatgc agaacgagcg cggcgaggga
      601 accaacaagg aggactacga gatcctcggg ccgctgccgc gcaagaacat cgacaccggc
      661 atgggggtcg agcgcatcgc gctggtgctg cagggcgtgc acaacgtcta cgagaccgac
      721 ctgctgcggc cgatcatcga tgtggtcgcc gcgcgcgcac cccgtcccta cgacgccggc
      781 aaccacgccg acgacgtgcg ctaccgcatc atcgccgacc acagccgcac cgcggccatc
      841 ctgatcggcg acggcgtcag cccgggcaac gacggacgcg gctacgtgct gcgccggctg
      901 ctgcgccggg tgatccgctc ggccaagctg ctgggcatcg aggacccgat cgtcggcgac
      961 ctgatggcga ccgtgcgcga cgcgatgggc ccgtcgtatc ccgagctcgt caccgatttc
     1021 gaccggatca ggcgcatcgc cgtcgccgag gagaccgcgt tcaatcgcac gctcgcatcg
     1081 ggttcgaagc tgttcgagga ggtcgccggc gccaccaagg cggacggcgc caccgtggtg
     1141 tccggttcgg acgcgttcac cctgcacgac acctacggct tcccgatcga gctcaccctg
     1201 gagatggccg ccgaggccgg cctgcaggtc gatgaatccg gcttccgcga gctgatggcc
     1261 gaacagcgca agcgcgccaa ggacgatgcc gccgcgcgca agcacgcgca cgccgacttg
     1321 agcgcctacc gtgagctggt cgacgccggc ccgaccgagt tcaccggatt cgacgagttg
     1381 acttccgagt caaggattct gggcatcttc gtcgacggca agcgggttcc ggtggtcgcg
     1441 cacgggaccg atggttccga ccgcgtcgaa cttgtgctgg accgcacccc gctctacgcc
     1501 gagtcgggtg gccagatcgc cgacgagggc accatcagcg gaaccggcgc caacggcagc
     1561 gcccgcgcgg cggtcaccga cgtccagaag atcgccaaaa ccctgtgggt gcaccgggtc
     1621 aacgtggagt cgggggaatt cgtcgagggc gacaccgtca tcgccgccgt ggacgcgggc
     1681 tggcgccggg gcgccaccca agggcactcg ggtacccaca tggtgcacgc cgcgctgcgg
     1741 caagtgttgg ggcccaacgc cgttcaggcg ggatcgctga accggccggg ctatctgcgc
     1801 ttcgacttca actggcaggg gccgctgacc gaagaccagc gcaccgagat cgaagaagtg
     1861 accaaccagg ccgttcaggc cgacttcgag gtgcacacct tcctcgaaaa gctcgatacg
     1921 gccaaggcga tgggcgcgat ggcgctgttc ggcgaggcct atcccgacga ggtgcgggtc
     1981 gtggagatcg gcggaccgtt ctccttggag ctgtgcggcg gaacccacgt gcacaactcg
     2041 gcccagatcg gcccggtgac gatcctcggc gagtcgtcga tcggctcggg catccgccgg
     2101 gtcgaggcct acgtcgggct ggattccttc cgccacctcg ccaaggagcg ggcgctgatg
     2161 gccgggctgg cgtcgtcgct gaaggtgccg tccgacgagg tgcccgcccg ggtggccaac
     2221 ctggtggagc ggttgcgggc cgccgagaag gaactcgaac gcgcccggct ggcgagcgcc
     2281 cgcgtcgccg cggccaacgc ggcagccggc gcggagcgga tcggtgacgt ccgtgtggtg
     2341 gtgcagcggg tgcccggcga gatgacggcc ggcgacctgc gctcgctggt gggcgatatc
     2401 cgcggcaagc tgggcagcga tcccgtggtg gtggcgctga tctcggtgaa ctccggcgac
     2461 gagggcacca ctgtgccgta tgcggtcgcc gccaatgcgg ccgcccaaga tctcgggttt
     2521 cgcgccaacg acctggtcaa actgctcgcc gccgccgtcg atggtcgcgg cggtggcaag
     2581 gcggatctgg cgcagggctc agggaaggat ccgaccggta tcgacgcggc gctcgacgcg
     2641 gtccgcgccc agatctccga gatagcg
//
LOCUS       DASSGQ010000205         2627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_622315,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000205 DASSGQ010000000
VERSION     DASSGQ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622315"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..671)
                     /locus_tag="VFI55_09050"
     CDS             complement(120..671)
                     /locus_tag="VFI55_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7074209.1"
                     /translation="MTKRRAHDPPPNRAELERRLASDMRAITAQSDRIGRHFARMNDV
                     SSSDFHALLHIMVGETAGTPLTLAQLRQRMDFSPAAITYLVDRMIDAGHIRRDPDPQD
                     RRKWLLRYEDDGMSLARDFFSPLGVHLNAALADLSEDDLSAAHRVFTAMIAALSTYGD
                     ELVNQPPKLRRVSTSPRRPQPPR"
     gene            complement(879..1316)
                     /locus_tag="VFI55_09055"
     CDS             complement(879..1316)
                     /locus_tag="VFI55_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7074210.1"
                     /translation="MVDFNGARLYVYARSLATVLRVDGEIDASNANNVANAIGRLARL
                     KTPLILDLSHLEFLSVDGFRALLVFYRAQQEARVHCSVVAGAAARPLLRIVGEHSLPT
                     AGSVAEALQLIEDIVGERRQFVSDIVRHRQPLREAAQGAPISP"
     gene            complement(1541..>2627)
                     /locus_tag="VFI55_09060"
     CDS             complement(1541..>2627)
                     /locus_tag="VFI55_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HET7074211.1"
                     /translation="VRWRHTHEFEEVGDDRTRVIDRVDTPVPGSLLRPMFAYRHRQLA
                     DDLAAHRLAAESGLRPVTVAITGSSGLVGSALTAFLRTGGHRVIQLVRRTPHGDDERR
                     WNPDDPDPDLLAGVDAVIHLAGASIAGRFTESHRDAIRASRIGPTRRLAELLGRGGPG
                     PAALISASAIGYYGYDRGDEILSEDSDRGDGFLADVVAEWEDATAPAERAGVRVVRVR
                     TGIVQSPRGGTLQLMRPLFSAGLGGRLGDGRQWLSWIGIDDLVDVYHRALWDTALTGP
                     VNAVAPQPVRNAEYTRTLASVLRRPAVLPVPSLGPRLLLGAQGARELACASQRVAPRK
                     LTESGHRFRQPDLDQALRHLLGRGGSG"
BASE COUNT          447 a          880 c          891 g          409 t
ORIGIN      
        1 accgagcatt tcagttattt aaccattaag caagttaact aatgccggag cgccgaaatc
       61 ggtggcccgg cggtgacgcc gctgtgggcc tgatcgagca gcgctcgccg cggccggttc
      121 taccgcgggg gttgcgggcg ccgcggggac gtgctgactc gccggagctt ggggggctgg
      181 ttgacgagct catccccgta cgtcgacaac gcggcgatca tcgccgtgaa aacccggtga
      241 gcggctgaca ggtcgtcttc gctgaggtcc gccagggccg cgttgagatg aacaccgagg
      301 ggactgaaga agtcccgcgc cagcgacatg ccgtcatctt cgtagcgcag caaccacttt
      361 cgtcggtcct gcgggtcggg atcgcggcga atgtggccgg catcgatcat ccggtccacc
      421 agataggtga tcgccgccgg cgagaagtcc atccgctgcc gcagctgggc caacgtcaac
      481 ggtgttcccg cggtttcgcc gaccatgatg tgcagcagcg cgtggaagtc gctgctactc
      541 acgtcgttca tgcgggcaaa atggcggccg atgcgatcgg attgcgcggt gatcgcccgc
      601 atgtcggacg ccaaccgcct ctccagctcg gcgcggttgg gcggtgggtc atgtgcgcgg
      661 cgcttggtca cctacccgca tccttcatca gctccaccgg catatgcgcg gcgatccgag
      721 acctacggcg atcggccgcg cgtcaggggt gaccctaccg gagtgatcgg gaaaatcacc
      781 gtcatgtccg gtcggagggc gcccgtgcgg atctggacct ggccgtcgcg agggcggcag
      841 cggaaacgaa agctgctgtc gttgagcgct ttaccctctc agggagagat cggggcacct
      901 tgcgcggctt cgcgcagcgg ttgtcgatgc cggacgatgt ccgacacgaa ttgacggcgt
      961 tcccccacga tgtcttcgat gagctgaagc gcttcggcaa ccgatccggc ggtgggcaag
     1021 ctgtgctcgc cgacgatccg aagcagcggg cgcgccgcgg caccggcgac cacgctgcag
     1081 tgcacccggg cctcttgctg cgcgcggtag aacaccagca gtgcccgaaa accgtcgacg
     1141 ctcagaaact ccagatgact caaatcgagg atcaacgggg tcttcagccg ggcaaggcgg
     1201 ccgatcgcat tggcgacgtt gttggcgttc gacgcatcga tctcgccgtc gacgcgcagg
     1261 acggtagcca ggctgcgcgc ataaacatat agtcgcgccc cgttgaaatc gaccacgtgg
     1321 cggcagcggt caggttggat tcggtccggt gaatctttgg aatcaaaagt attcatgtga
     1381 ccagcccgtc gaccgtgatg cgagggagtg actcgtgttc tacacaccgc tcccgaggac
     1441 accaagctgt gaactcaact ggcaaaagcc taccgccgac ggcggtgcgc aggtaccgaa
     1501 atgttgcggc agccaggccc tgatagcacg acggcttcga tcagccagac ccgccgcgcc
     1561 cgagcaggtg acgcagcgcc tgatcgaggt cgggctggcg aaaccggtgc ccggactcgg
     1621 tgagcttccg gggcgccact cgttgactgg cgcacgccag ttcgcgggcg ccctgcgcgc
     1681 ccagcaatag ccggggcccc agcgacggca ccggcaacac cgcgggccgg cgcagcacgc
     1741 tggccagggt gcgcgtgtac tcggcgttac gaaccggttg cggggcaacg gcattcaccg
     1801 gcccggtcaa ggcggtgtcc cacagcgcgc ggtgatagac gtcgaccaga tcgtcgatcc
     1861 cgatccagga cagccactgc cggccgtcgc cgagccggcc gcccagcccg gcgctgaaca
     1921 gcgggcgcat cagctgcaga gtgccaccgc gaggggattg cacgatgccg gtgcgcaccc
     1981 gcaccacccg caccccggcc cgctccgcgg gcgcggtcgc gtcctcccac tcggcgacga
     2041 cgtcggccag aaatccgtcg ccgcgatcgc tgtcctcgga cagtatttcg tcgccgcggt
     2101 catatccgta gtacccgatc gccgacgccg agatcagtgc ggcaggtccg gggccgccgc
     2161 ggcccagcag ctcggccagc cgacgggtcg gcccaattct gctggcgcgg atcgcatccc
     2221 ggtggctctc ggtgaaacgg ccggcgatcg aggcacccgc cagatggatc accgcgtcga
     2281 ccccggcgag caggtcgggg tcagggtcat cgggattcca ccgccgctcg tcgtcgccgt
     2341 gtggggtccg gcgcaccagc tggatgacgc gatggccgcc ggtgcgcaga aacgcggtca
     2401 gcgccgatcc gaccagtccc gacgatcccg tgatcgcaac cgtgacgggg cgcaaaccac
     2461 tctcggcggc cagccggtgc gcggccagat cgtcggccag ctgacggtgg cggtaggcga
     2521 acatcggccg cagcaacgac ccgggtaccg gcgtgtcgac ccggtcgatc acccgggtcc
     2581 ggtcgtcgcc gacctcttcg aattcgtggg tgtgccgcca ccgcacc
//
LOCUS       DASSGQ010000206         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_45070, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000206 DASSGQ010000000
VERSION     DASSGQ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45070"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VFI55_09065"
     CDS             complement(<1..447)
                     /locus_tag="VFI55_09065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7074212.1"
                     /translation="MNTAPSAAPIGSTYVLGHADVEVQRLLLQGRLYNDYTEHALRLA
                     GLRPGMRVLDIGCGPGDVSLIAARLVGPTGTVLGVDAAPEMIELARGRAAEQGLPTVH
                     FTQAAIDTIALDEPVDAVIGRLILMHLPDPAATVRHLSSFVRPGGVV"
     gene            complement(444..1532)
                     /locus_tag="VFI55_09070"
     CDS             complement(444..1532)
                     /locus_tag="VFI55_09070"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HET7074213.1"
                     /translation="MNNRVLITGATGRIGGAVATQLLEKGVTTRAMVHRDDARSARLR
                     DLGAEIVVADMFDIQQVTAAMDGVNRLYFNPPYHPHALDSAVAFAVAARRNGVEAVVA
                     LGQWLASPEHPSLMTRQAWLTDKLFELLPDTAHVAVDPGYFADNYLQVLPMAAQLGVL
                     PTPTGAGRNAPPSNEDIARVAVGALLDPHRHDGRAYRPTGPKMLSGAEIADAVGEALG
                     RRVRHIDIPQWMFMRAVRVNAKRLGTDVFFESSLRHYLPEYPLGTFEVGGPTTHVRDV
                     AGVEPEDFLTIARRYVTGPESRRTPGNLVRSIWDFMLTGLVPMHRLDKFDRLQQHPQP
                     AHPRLSGESAVWRNEHTVAAGAERMEAR"
     gene            complement(1529..>2062)
                     /locus_tag="VFI55_09075"
     CDS             complement(1529..>2062)
                     /locus_tag="VFI55_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7074214.1"
                     /translation="AVVAAGLTVFAAGLAWASTADAATPYGQIATQMLLLGGGLGLTT
                     APATEAIMGSLSVDKAGVGSAVNDTTRELGGTLGVAIAGSIFASVYSGHLGSAALTGL
                     PAESMRHSMAVAHKVIEQLPAQRAGDVRAAVDHAFLDGLQVSALVCAGIALGAAIVVG
                     WLLPAWEPARQLEREIR"
BASE COUNT          335 a          691 c          699 g          337 t
ORIGIN      
        1 gacaacaccg ccggggcgca cgaaggaact taagtgccgc accgtggcgg ccgggtcggg
       61 caaatgcatc aggatgagcc gcccgatcac cgcgtcgacc ggttcgtcca aggcgatcgt
      121 gtcgatcgcg gcctgcgtga aatgcactgt aggcaagcct tgttcggcgg cgcggccgcg
      181 ggcgagctcg atcatctcgg gcgcggcgtc cacacccaga acggttccgg ttggaccgac
      241 cagccgggcg gcgatcaacg agacgtcacc gggcccacag ccgatgtcga gcacgcgcat
      301 gcccggccgc agtccggcaa gccgcagcgc atgttcggtg tagtcgttgt acagccgtcc
      361 ctgcaggagc aggcgttgca cctccacgtc ggcatggccc aggacatacg tgctgccgat
      421 cggtgcggca gacggcgccg tattcatcgc gcttccatcc tttctgctcc ggccgcgacg
      481 gtgtgctcat ttcgccacac cgcggattca cccgaaagcc gggggtgtgc gggctgtggg
      541 tgctgttgga gccggtcaaa cttgtccagc cggtgcatgg gcaccagtcc ggtcaacatg
      601 aaatcccaga tgctgcggac gagattgcct ggggtgcgcc gggattccgg tccggtgacg
      661 tagcggcgtg cgatcgtcag gaagtcttcg ggctcgacac cggcgacgtc acgcacatgc
      721 gtagtcgggc cgcccacctc aaaggttccc agtgggtact cgggcaggta gtggcgcagg
      781 ctggactcga agaacacgtc ggtgccgagt cgtttggcgt tgacgcgcac ggcacgcatg
      841 aacatccact ggggaatgtc gatgtggcgc acccgccggc ccaacgcttc gccgaccgcg
      901 tcggcgatct cggctccgga caacatcttc ggccctgtcg gacggtaggc gcgaccgtca
      961 tgacggtgcg ggtcaaggag cgcacccacc gccacccggg cgatgtcttc gttggagggt
     1021 ggcgcgtttc ggcccgcccc ggtcggcgtc ggcagcactc ccagctgggc cgccatcggt
     1081 aacacctgca ggtagttgtc ggcgaagtag ccggggtcaa ccgccacgtg cgcggtgtcc
     1141 ggcagcagct cgaacaactt gtcggtcaac caggcctgcc gggtcatcag cgacggatgc
     1201 tccgggctgg ccagccactg cccaagcgcc accaccgctt ccacgccgtt gcgtcgcgcg
     1261 gccacggcga acgcgaccgc gctgtcgagc gcatggggat ggtatggcgg gttgaagtaa
     1321 agccggttca caccgtccat cgcggccgta acctgttgga tgtcaaacat atcggcgacg
     1381 acgatttcgg cgcccaagtc ccgcaaccgg gcgctgcggg cgtcatcacg atgcaccatg
     1441 gcccgggttg tgacgccctt ctccagcagc tgggtggcga ccgcaccccc gatcctgccc
     1501 gtggcaccgg ttatcaatac gcgattgttc atcggatttc cctttccaat tgacgtgccg
     1561 gctcccaggc cggcagcagc cagccgacga cgatcgctgc ccccagcgcg atgcccgcac
     1621 ataccagcgc gcttacttgg aggccgtcca ggaatgcgtg atcgacggct gcgcggacgt
     1681 cgccggctcg ttgcgcggga agctgctcga tcactttgtg cgccactgcc attgagtgcc
     1741 gcatcgactc ggcgggtagc cccgtcaacg cggccgagcc cagatgtccc gagtagaccg
     1801 atgcaaagat gctgccggcg atggcgactc cgagggtgcc tccgagttca cgcgtcgtgt
     1861 cgttgacggc cgagccgact cccgccttgt cgacggagag ggatcccatg atcgcctcgg
     1921 tggcgggcgc cgtggtcagg ccgaggccgc cgccgagcag cagcatctgc gtggcgatct
     1981 gaccgtaggg cgtggccgcg tccgctgtgg acgcccacgc cagcccggcg gcgaacacgg
     2041 tgaggccggc ggcgacgacg gc
//
LOCUS       DASSGQ010000207        11160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1503404,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000207 DASSGQ010000000
VERSION     DASSGQ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11160
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503404"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1339
                     /locus_tag="VFI55_09080"
     CDS             <1..1339
                     /locus_tag="VFI55_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HET7074215.1"
                     /translation="KLTQLGTAVGTWKYMAPERFSNDEVTYRADIYALACVLHEALTG
                     SPPYRADSAGTLVTAHLMDPIPQPSTLRAGIPKAFDAVIARGMAKKPQDRYASAGDLA
                     LAAHEALSDPDQDHAANILRRSQEATLPTSTDPTAVASAQPQPPVSTPPPAPPTPTPY
                     PQVPQAWTPDSGPIPAPSQPAGHYYQPHNANWAGPPVSPPHQFAASPSPWNQTPAKPQ
                     RNPWPIIAAVAVVLVLIVGGVGIWSVTRPKPAPPVDPVPTDRLSALLLSPSDIDSVMG
                     SSTMEPGKPITSMDSSSVTLSAPACQGALYTSQDPVYAGTGYTGVSGLVSSEPGDNYD
                     HWVNQAVVAFPSADKAKAFLQTSADKWKTCAGRTVTVTNKGKTYRWTFAQINGSPPKI
                     AVLDTQEAADGWECQRAMGVANNVIVDVNACGYHITDEGGQITDKIVDKIRNA"
     gene            complement(1397..1990)
                     /locus_tag="VFI55_09085"
     CDS             complement(1397..1990)
                     /locus_tag="VFI55_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074216.1"
                     /translation="MKPHHTHGRRYGRPGGWQQAQQPDASGAAEWFAGRLPEAWFDGD
                     PTVVVDREEITVIGKLAEPEGSGNEESGARAEGRVSRFREETRSERMRIADEAQERYA
                     RKVSWAVEVGGERILFTHIAVPVMTRLKQPERQVLDTLVDAGVARSRADALAWSVKLV
                     GEHSEEWLAKLRTAMSAVDDLRAEGPDLRAEGPDIQV"
     gene            complement(2281..3414)
                     /locus_tag="VFI55_09090"
     CDS             complement(2281..3414)
                     /locus_tag="VFI55_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7074217.1"
                     /translation="MDPCAAQCTDDLLNGLEGTARSQRAELVQWLLDQGITADEIRAN
                     NPPLLLASRHLIGDDGTYVSTREISETYGIDLMLLQRIQRAIGLARVDDPDAAVHMRA
                     DGEAAAFAQRFVELGLSPDQLVLVVRVLAEGLSRAAEVMRYAALAAIIRPGATELETA
                     KASKELVSQIAPIMGPMIQQMMFMQLRHMMETESVNAGERAAGKPLPGARQITVGFAD
                     LVGFTRLGEVVSAEELGHLANRLADLARDVTTPPVRFIKTIGDAVMLVCTDPVPLLDV
                     LLKLVDAVDTDNAFPRLRAGVASGMAVSRAGDWFGSPVNMASRVTGVARPGAVLVADS
                     VWEAIGATGRFDGSFAGPRRLKGIKNDVKLFRIRRGDGAGTGC"
     gene            complement(3668..5059)
                     /locus_tag="VFI55_09095"
     CDS             complement(3668..5059)
                     /locus_tag="VFI55_09095"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET7074218.1"
                     /translation="MDARDVAFAGAAAQARMLADGELSAPELLEIYLERIARLDSQLR
                     CYRVVLSDTARYEAAVAQDRIDAGERLPLLGVPIAIKDDVDIAGEVTTYGSGGHRPAV
                     TSDAEVVHRLRAAGAVLIGKTNVPELMMFPYTESLTFGATRNPWNPKRTPGGSSGGSA
                     AAVAAGLAPLALGSDGGGSIRIPAIWCGLFGLKPQRDRVSLEPHDNAWYGLSVNGPIA
                     RSVLDAALFLDATTKVPGPEGEFAAAAARHPGQLRIALSTKIPTPLPVRVGKEQLAAV
                     DQAGALLRDLGHEVIIRDPEYPVSAIFANYLPRYLRGICDDADAQAHPDRLEARTRNL
                     ARLGAFFSDRRMDAVQAAESSLTTRIQSIFDDVDVVVTPGNATGPSRIGAYQRRGAVS
                     TLLLVAQRVPYQQIWNVTGQPVAAVPWDFDGDGLPIAVQLVGRPYDEATLLSLSAQIE
                     AARPWAHRRPPVS"
     gene            5126..5560
                     /locus_tag="VFI55_09100"
     CDS             5126..5560
                     /locus_tag="VFI55_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET7074219.1"
                     /translation="MSCVFCAIVAGGAPATRIYEDDGYLAILDIRPFTRGHTLVLPKQ
                     HTVDLTDTPPETLADMVVIGQRIARAARATELADATNIGINDGRAAFQTMFHIHLHVL
                     PRRNGDKLSVARGMLLRRDPDREATARILREALARIDASQSD"
     gene            5732..9733
                     /locus_tag="VFI55_09105"
     CDS             5732..9733
                     /locus_tag="VFI55_09105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7074220.1"
                     /translation="MKRWTGKPWRRVRLPARAGRLVFVVYGVAAVLVATRSTFGGEGA
                     FATRRVNDVVSVLCLVFAAACAGYAARFAVGSYRVGWLALVTALLGWAGGEVISAVYR
                     VRPALDHATHPVAAQAVFLLYPIGALASLAALSHLTLHRPLRLVLDGLIVATSLFVIS
                     WEFLLAKQLSGESGSAWPATLTQVSADVVLMTTAILLLSRFRPGDPPSRSLLAGGITT
                     IAVAHVTTMFQSGTGSYHTGGLADLGRVAGLGLMALAALSSVNEPPTAPPRQAIASRA
                     RLWLPYLPLLVAAAVGWRYTVGQMHHGPMPALEGILVGAVLVRQFVVLFENQSLLSEV
                     AREAFRDSLTGLANRAYFVNRLEQVVTHRGHDAIPIAVLCLDLDNFKSVNDALGHPAG
                     DELLVRVAARLTAAVSDTGTVARLGGDEFAVFMQGSLEESQAMAHRVLEAFTAVIVID
                     GVPIEVRPSIGFTVAAPESDCTVDQLLRHADLAMYAAKREGGQCIRGFVPDQPFPYTF
                     LQYADEEESPALDAIIAPAYQGAVATAGKEAAPPPARKPIAQIATPPREQLYDTPTGI
                     RWPPTSIRTALALLIIGVTAYSLSRVFGMDAGHSAFFTKFLYSALNLSAAALIAVRAY
                     RVMSERSAWILIAAGMTCSAVGDVVYALWVPDGQSPSVADPAYLAFYPFVYAGLLLLM
                     RARRKRVPIALRLDSLVCGFTMAALAAALTAGPIRAAAARAPSTVLVGVLYPWGDLVL
                     LALAAGMLPIFRWRNEFRWGLLIAGLMGFAVADTAYLFQTAAGSYRVGSALDACWPAS
                     SLLLAMASWAPSSSVVPIPRRGFGPYIKPVACTVVALGVAILAHNSRLAVTLAALSLV
                     AIAARFAVTFRDVSMLAENHKRAMTDELTGLPNRRSLATALTALSGVSTTPTSVTRTS
                     SRRALLLLHLCEFHEINESVGHRFGDELLRHIANRLANSVRRDDLLARTGDDEFAILL
                     AEGSDLMAARAQAGWLLEAFNEPFALDPITVQVDARIGIAVWPDHCDHPLELLNRAET
                     TIPHAKSAIRKITVYNPAFEVSRDNDPNVVEELRAALFDGDQLTVHYQPKINASDGSV
                     HSVEALLRWQHPNRGLLLPEEFLPAAERAGLMRKVANRTINRALEQVVAWHGDGIPLT
                     VAVNLSTTNLLDLDLVGTIERLLRTHCLPADTLIVEITESALVDSVRSRSTIAALQRL
                     GVRISLDDYGTGWSSLARLQDVSVDELKLDRVFVARLAQDPRSVAIVRSTVALAQSLG
                     ADLVAEGVEDEVTLSALRRYGCTITQGFVHSPPLPPDDLRRWIATHVPNGKPSEPKQA
                     AVTTDRSEQFFGE"
     gene            complement(9710..10741)
                     /locus_tag="VFI55_09110"
     CDS             complement(9710..10741)
                     /locus_tag="VFI55_09110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7074221.1"
                     /translation="MHLSVLDLVPVRSDQNTRDALAATVQLAQTADRLGFTRYWVAEH
                     HNMPSVGATSPPVLIAYLAAQTVQLRLGSGGVMLPNHAPLAVAEQFALLEAAAPGRID
                     LGIGRAPGSDPVTSYALRGTNDDRDIENFPNYLDDVAALMSARGVVVPLRSGDYRLKA
                     TPAAVGEPRLWLLGSSMYSANLAAAKGLPYVFAHHFSGKGTEEALELYRSRFVPSDLT
                     PEPVTFLTVNAAVAETSDEATALMLPNLQMMARLRTGQPLGPVPLVEEAAAAHVSPDQ
                     QRIVDSGLQRAILGTPSEAAEQLRALAQRFDVDEVMVHPVASAYRGTDPATAPARVAT
                     LELLAKELF"
     gene            complement(10793..>11160)
                     /locus_tag="VFI55_09115"
     CDS             complement(10793..>11160)
                     /locus_tag="VFI55_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7074222.1"
                     /translation="VRPQLLRYMRTAPDFYFDEMSQIKMDRWSKGRVALVGDAAYCCS
                     PLSGQGTSVALLGAYILAGELAAAGDDHELGFTNYHKEFQDYVKRNQWLVVDNIPGGA
                     PIPQEVFDRIVNSITPKDY"
BASE COUNT         1856 a         3860 c         3575 g         1869 t
ORIGIN      
        1 aaaactcacc caactcggca ccgccgtggg cacctggaaa tacatggccc ccgaacggtt
       61 ttccaacgac gaggtcacct atcgcgccga catctacgca ctggcctgcg tactacacga
      121 ggcactcacc ggatccccgc cctatcgcgc cgacagcgcc ggcactctgg tcaccgcgca
      181 cctgatggac cccatccccc aacccagcac cctgcgcgcc ggcatcccca aagccttcga
      241 cgccgtcatc gcacgcggga tggccaaaaa accccaggac cgctacgcca gcgccggcga
      301 cctggccctg gccgcccacg aagccctgtc cgacccagac caagaccacg ccgccaacat
      361 tctgcgccgc agccaagaag ccaccttgcc gaccagcacc gaccccaccg ccgtagcgtc
      421 cgcgcagccg caacctccgg tcagcacccc accaccggcg ccacccacgc ccacgcccta
      481 cccccaggtc ccccaggcct ggaccccgga cagcggccca atacccgccc ccagccaacc
      541 cgccggccac tactaccagc cccacaacgc caactgggcc ggcccgcctg tcagcccacc
      601 acaccaattc gccgccagtc cgtcgccctg gaaccaaacc ccggccaaac cccaacgcaa
      661 cccctggccg atcatcgccg ctgtcgcagt tgtgctggtt ctcatcgtcg gcggtgtggg
      721 catctggagt gtcacccggc ctaaaccggc gccgccggtc gacccggtac cgaccgatcg
      781 tctcagcgcc ttgctgctga gcccgtcgga tatcgactcc gtgatgggct cgtcgacgat
      841 ggagccgggc aagcccatca catcgatgga cagctcgtcg gtgacgctgt cggcgccggc
      901 ctgccagggc gcgctgtata ccagccagga tccggtgtac gcgggcacgg gctacaccgg
      961 cgtgagcgga ctggtgtcct ccgagccggg ggacaactac gaccactggg tcaaccaggc
     1021 cgtcgtcgcg tttccgtccg ccgacaaggc caaggcgttt ctgcagactt ccgccgacaa
     1081 gtggaagacc tgcgcgggca gaacggtcac cgtgacgaac aagggcaaga catataggtg
     1141 gacgttcgcg caaatcaacg gcagtccacc gaagatcgcg gtgctggaca cccaggaggc
     1201 cgccgacggc tgggagtgcc agcgcgcgat gggcgtggcg aacaacgtga tcgttgacgt
     1261 caacgcgtgc gggtaccaca tcaccgacga gggcggacaa atcaccgaca aaatcgtcga
     1321 caagatcagg aacgcgtagc gcgactgcta cccgagccag tgctcgccaa tgccccaggg
     1381 gcactgcggc attggcttac acctgaatat ccgggccctc tgcgcgcaaa tccgggccct
     1441 cggcgcgcaa atcgtcgacc gccgacatcg cggtgcgcaa cttcgccaac cactcctcgg
     1501 agtgctcacc gaccagcttg accgaccagg cgagcgcatc ggcgcgggac cgggcaacgc
     1561 cggcgtcgac caacgtgtcg agcacctggc gttcgggttg cttcaagcgg gtcatcaccg
     1621 gtacggcgat gtgagtgaac aggattcgct cgccaccgac ctccacggcc caagaaacct
     1681 tgcgcgcgta gcgctcctgc gcttcatcgg cgatgcgcat ccgctccgaa cgggtttctt
     1741 cgcggaaccg tgagacgcgg ccctcggcgc gggccccgct ctcctcgttg ccggaaccct
     1801 cgggttcggc cagctttccg atgacggtga tctcttcgcg gtccacgacg accgtgggat
     1861 cgccgtcgaa ccaggcttcg gggaggcgtc cggcgaacca ttcggccgcg ccgctcgcgt
     1921 cgggctgctg cgcttgttgc cagccgccgg ggcggccgta gcgccgtccg tgtgtgtgat
     1981 gaggtttcat gattacatga ttacatgctt acacgtgtaa tggaattgcg ttcactggca
     2041 gcgaacgcga aacgcatgta gccggcgcgt cgctcccgac cgcttcagag ccccggcact
     2101 gaaaacccgt tgatatgggc tgttcggagt tgtatttttg atagcgaagc aggcccggaa
     2161 gcgaccaatt cgaagcgccg gaagacgggg tagcaggccg gccgcgtatg acgcatcgtc
     2221 aaacgccccc gagagtcgcc cgggcctgcc catgcttctt cccagtcgcg atcagccggt
     2281 tcagcagccg gtgcccgcgc cgtcccctct gcggatccga aaaagtttga cgtcgttttt
     2341 gatgcccttg aggcggcgtg gaccggcgaa ggacccatcg aatctgccgg tggcgccgat
     2401 ggcttcccag accgaatccg cgaccagcac tgcacccggg cgcgccacac cggtcacgcg
     2461 gctggccatg ttgaccggac ttccgaacca gtcgccggcc cggctcaccg ccatgccgga
     2521 ggccactccc gcgcgcaacc gcgggaacgc gttatcggtg tcgacggcat cgacgagctt
     2581 cagcaggaca tccagcagtg gcaccggatc ggtgcagacc aacatcaccg cgtcgccgat
     2641 cgtcttgatg aaccgcacgg gcggggtggt cacgtcacgg gcgaggtcgg ccagccggtt
     2701 ggccaggtgc cccagctctt ccgccgatac cacttcgccc aaccgggtga agccgacgag
     2761 gtcggcgaag ccgacggtaa tttggcgcgc tcccggaagc ggtttccccg cggcccgctc
     2821 accggcattg accgactcgg tctccatcat gtgccgcagt tgcatgaaca tcatctgctg
     2881 gatcatcggg cccatgatgg gcgcgatctg gctcaccaac tccttggacg ccttggcggt
     2941 ctccaactcg gtggcgcccg ggcggatgat cgccgccagt gcggcgtaac gcatgacctc
     3001 ggcagcgcgt gatagacctt cggcgagcac ccggaccacc agcaccaatt gatccggact
     3061 caggcccaac tcaacgaacc gctgggcgaa ggcggcggct tcaccgtcag cgcgcatgtg
     3121 aaccgccgca tcggggtcgt ccactctggc cagaccgatg gcgcgctgta ttcgctgcag
     3181 cagcatcagg tcgatgccgt aggtctcgct gatctcccgg gtagacacat acgtgccgtc
     3241 gtcgccgatg aggtgacggc tggccagcaa cagtggcggg ttgttcgcgc ggatttcgtc
     3301 ggcggtgatg ccttggtcga gcagccactg cacgagttcg gcccgctggc tgcgggcggt
     3361 gccctcgagc ccgttgagca ggtcatctgt gcactgggcg gcgcacgggt cgatcctgtc
     3421 gtgaccgtgg tcctgcacga tttcaacgta caacgcggcc agccatgatg tgacccgatg
     3481 agcgcaccac ttcggccggg attcccgccg gtcgacgacc cggcccgccg ggatgtgcag
     3541 cacagctccc gccgcgtcgg cagcgccgac ggacggtcga cccgaaaact ttggtggtca
     3601 acggctttgg cgagccgttc gccagctgga cgccgctacg gccgccgagc cgcaccgaat
     3661 ttctgactca cgacaccggt ggtcgccgat gggcccacgg ccttgcggct tcgatctgcg
     3721 cggacaacga caacagcgtc gcttcgtcgt acggccgccc gacgagttgc acggcgatcg
     3781 gcaagccgtc accgtcgaaa tcccatggca cggcggcgac gggctgcccc gtcacattcc
     3841 agatttgttg gtagggaacc cgctgcgcca ccagcagcag cgtcgacacc gcaccccggc
     3901 gttggtaggc gccgatgcgg gacgggcccg tggcattccc cggtgtgacg acgacgtcga
     3961 cgtcgtcgaa gatcgactga atccgggtgg tgagcgagga ttcggcggcc tggaccgcgt
     4021 ccatgcgccg gtcggagaag aatgcaccga ggcgtgccaa gttgcgggtg cgcgcttcaa
     4081 gacgatccgg atgagcctgc gcgtcggcgt cgtcgcagat accgcgcaga taacggggca
     4141 ggtagttcgc gaagatcgcc gaaaccggat actccggatc gcggatgatg acctcatggc
     4201 ccaaatcgcg aagtagggcg ccggcctggt caactgccgc cagctgctcc ttgccgaccc
     4261 ggacgggcag cggcgttggg atcttggtgc tcaaggcaat tcgcaactgg ccgggatggc
     4321 gcgccgccgc ggccgcaaac tcgccttcgg gaccgggcac cttcgtcgtc gcatccaaga
     4381 acagcgccgc atccagcacg gaccgggcta tcggcccatt gacgctgagt ccgtaccagg
     4441 cattgtcgtg cggctccaac gacactcgat cgcgttgcgg tttcaggccg aacagaccgc
     4501 accagatcgc ggggatgcga atcgaaccgc caccgtcgga tcccagcgcc aggggggcca
     4561 gcccggcggc caccgcggcg gcactaccgc cgctgctgcc gcccggcgtg cgtttcgggt
     4621 tccacgggtt gcgcgtggcc ccgaacgtca gcgactcggt gtagggaaac atcatcagct
     4681 cgggcacgtt ggttttgccg atcaggaccg cgccggcggc gcgcagccga tgaaccacct
     4741 ccgcgtcgga cgtcaccgcc gggcggtggc cgccgctgcc gtatgtcgtc acttcgccgg
     4801 cgatgtcgac atcgtctttg atcgcgatcg gcacgccgag cagcggcagc cgctcgccgg
     4861 cgtcaatgcg gtcctgcgcc acggcggcct cgtaccgtgc ggtgtcggac agcaccacgc
     4921 ggtagcagcg cagctggctg tccaggcgcg caatgcgttc caggtagatc tccagcagtt
     4981 cgggtgcgct cagttcaccg tcagccagca tccgtgcctg cgcggccgca ccggcgaagg
     5041 ccacgtcgcg agcgtccact gcggcagcgt atcccggtcg cccagcaagg cacggcgtat
     5101 tgggtcaacc agtagcgtgg gcgacatgtc ctgcgtgttc tgtgcgatcg tcgccggggg
     5161 agccccggcc acccggatct acgaagacga cggctacctg gccatcctgg acatccgccc
     5221 gttcacccgc ggccacaccc tcgtgctccc gaagcaacac accgtagatc tcaccgacac
     5281 gccgccggag acgctggccg acatggtcgt catcggccag cggatcgcca gggctgcccg
     5341 cgcgacggaa ttggccgacg cgacgaacat cgggatcaac gacggccgcg ccgccttcca
     5401 gacgatgttc cacattcacc tgcacgtgct gccgcggcgc aacggcgaca agctatcggt
     5461 ggccaggggc atgctgctgc gccgcgaccc agaccgggaa gccaccgcgc gcatcctgcg
     5521 ggaggccctg gcccggatcg acgcgagtca gtcagactga gctgacgagc aatctcccca
     5581 tcccgatctt cgtgctgacg ccgcgttagt tgtggtcgag cagcaggtag cacaggtagt
     5641 gagcctgatg acagcccgga ggactattct cctgtagcgc ggtgatgctt tccatatgct
     5701 caccgcaagt gcgggatcgg ctggagctgc gatgaaacgc tggacgggaa aaccgtggcg
     5761 gcgggtgcgg ctgcccgcac gcgccggtcg gctcgtcttc gtggtgtacg gcgtcgcggc
     5821 ggtcctcgtt gcgacgcggt cgacgttcgg cggggagggt gccttcgcga ctcgtcgggt
     5881 caacgacgtg gtgtcggtgc tgtgtcttgt gttcgcggcg gcgtgtgccg gctatgcggc
     5941 tcgcttcgcg gtcgggagct accgagtcgg atggctggcg ctggtcacgg ccctgctcgg
     6001 ctgggcaggg ggtgaggtca tctcggcggt ctatcgggtg cgcccggcgc ttgatcacgc
     6061 aacccacccc gttgcggctc aggccgtgtt cctcctgtat ccgatcggcg ccttagcgtc
     6121 attggcggcg ctgagccatc tgactctgca ccgtcccctg cgactggtgt tggacggtct
     6181 catcgtggcg acttcgctgt tcgtcatctc gtgggaattc ctgctcgcga aacaactgag
     6241 tggggagagt ggctctgcct ggccggcgac tctcacgcag gtttccgccg atgtcgtcct
     6301 tatgacgacc gcgatcctgt tgttgtcccg gtttcgcccc ggcgacccgc ccagccggag
     6361 cctgttggcg ggcgggatca ccacgatcgc cgttgcccac gtcacgacga tgttccaaag
     6421 cgggaccggc agctatcaca ccgggggact ggccgacctc ggccgcgtgg cgggactggg
     6481 actaatggca ctggcggccc tgtccagcgt gaacgagcca cccaccgcgc caccgcggca
     6541 agcgatcgcc tcccgtgccc gactctggtt gccctacctg ccgctgctgg tggccgccgc
     6601 ggtcgggtgg cgctacacag taggccaaat gcaccacgga ccgatgccgg ccctcgaggg
     6661 aatcttggtc ggtgcagtgc ttgtccggca gttcgtggtg ctcttcgaaa atcagagcct
     6721 gttatctgaa gtcgcgcggg aagcgtttcg cgacagtctc accggtctcg ccaatcgggc
     6781 atacttcgtc aaccgtttgg aacaggtcgt cacgcatcga ggtcacgacg cgataccgat
     6841 cgcggtgttg tgcctcgacc tcgacaattt caagtcggtc aacgacgccc tgggtcatcc
     6901 ggccggcgac gagctgcttg tccgggtcgc ggcgcggctc accgccgcgg tgagtgacac
     6961 gggcaccgtc gcgcggcttg gtggtgacga attcgcggtc ttcatgcaag gttccctcga
     7021 agaatcacaa gcaatggcgc accgagttct cgaggcattc accgccgtga tcgtgataga
     7081 cggtgtgccc atcgaggtcc gtccgagcat cggcttcaca gtggccgccc ctgagtcgga
     7141 ttgcaccgtc gaccagctcc tccggcacgc cgacttggcc atgtacgccg ccaaacgcga
     7201 aggcggccaa tgtatccgcg gcttcgtacc ggatcagccc ttcccgtata ccttcctgca
     7261 gtacgccgac gaggaagagt ccccggccct agacgcgatc atcgcccccg cctaccaggg
     7321 cgccgtggcc accgccggca aggaggcggc gccgccgccg gcgcgcaagc cgatcgccca
     7381 gatcgcgacg ccaccgcgag agcagctgta cgacacgccc accggcattc ggtggcctcc
     7441 cacgtcaatc cgaactgccc tggcgctgtt gataatcggc gtgacggctt actccttgtc
     7501 ccgcgttttc ggcatggacg ccggtcacag cgcgttcttc accaaattcc tttattcggc
     7561 gttgaatctc tcggcggcgg cattgatagc cgtccgcgct taccgcgtca tgtccgagcg
     7621 gtcggcgtgg attctgatcg cggccggcat gacatgttcg gcggtgggcg acgtcgtcta
     7681 cgccctgtgg gtgccagacg gtcagtcccc gtcggtggca gatccggcgt acctggcttt
     7741 ctatccgttt gtctatgccg ggctgctgct gctcatgcga gcacgccgca agcgagtgcc
     7801 catcgccctc cggctcgatt cgctggtttg cggtttcacg atggccgcgt tggccgccgc
     7861 cttgacagct gggcctatcc gcgcggcggc ggcccgcgca ccgtcgaccg tgctggtggg
     7921 cgtgctgtat ccgtggggcg acctggtcct gctggccctc gccgcgggca tgctgccaat
     7981 cttcagatgg cgcaacgagt tccgttgggg cctcctcatt gccggactta tggggtttgc
     8041 ggtcgccgac acggcgtatc tcttccagac cgccgccggc tcataccgcg ttggcagtgc
     8101 gctggatgcg tgctggcccg cttcgtcatt gctgctggcg atggccagct gggcaccctc
     8161 gtcgtcggtg gtgcctatac cgagacgcgg attcggcccc tacatcaagc cggtggcgtg
     8221 cacggtcgtc gcccttggcg tcgccatcct ggcccacaac tcgcgactcg cggtgaccct
     8281 cgccgcattg agcctggtgg cgatcgcggc gcgattcgcg gtgactttcc gcgacgtcag
     8341 catgctggcc gaaaaccaca agcgggccat gaccgacgaa ttgaccggcc tgcccaatcg
     8401 gcgctcgctg gcgacagccc tgaccgcgct gtcaggcgta tcgaccacgc cgacatcggt
     8461 gactcgcacg tcctcacgtc gggcgctgct gttgctgcat ctctgcgagt tccacgagat
     8521 caacgaatca gtgggacacc gcttcggcga tgaactgttg cgccacatcg cgaaccggct
     8581 cgcaaacagc gtgcgccgcg atgacctgct ggcgcgcacg ggtgatgacg aatttgccat
     8641 cctgctggcc gaagggtccg acctgatggc cgcgcgcgcc caagcggggt ggctgctcga
     8701 agcattcaac gagccgttcg cgctcgatcc gatcaccgtg caggtcgatg cccgcatcgg
     8761 gattgcggtg tggcccgacc actgtgatca cccactggag ctgctgaatc gcgccgagac
     8821 gacgatcccg cacgcgaaat ccgcgatcag gaagatcacg gtgtacaacc cggcgtttga
     8881 ggtctctcgc gacaacgacc ccaacgtcgt cgaggaacta cgcgccgccc tgttcgacgg
     8941 tgaccaactc acggtgcatt accagcccaa gatcaacgcg agcgacggta gcgtccacag
     9001 cgtcgaggcg ctgctgcgct ggcagcaccc caaccgcggg ttgttgctac cggaggagtt
     9061 cctgcccgcc gccgagcgcg ccgggctgat gcgcaaggtg gccaatcgca cgatcaaccg
     9121 cgccctcgag caggtcgtgg cctggcacgg cgacggtatt ccgctgacgg tcgcggtgaa
     9181 cctctcgacg acgaacctgc ttgatctcga cctggtcggc acgatcgagc ggctgctgcg
     9241 tacccactgc ttacccgccg acaccctcat cgtcgagatc accgaaagcg ccctggtcga
     9301 ctcggtgcgg tcccgcagca ccatcgccgc gctgcagcgc ctcggcgtcc gtatatcgct
     9361 tgacgactac ggcaccggat ggtcgtcgct ggcacgcttg caggatgtct cggtcgacga
     9421 gctgaaactc gacagggttt tcgtggcgag actggcccaa gacccgcgct cggtcgccat
     9481 cgtgcgatcc acggtggcgc tcgctcaaag tctcggcgcc gacctggtcg cggagggtgt
     9541 cgaagacgag gtcacgttga gcgcactgcg gcgatacggc tgcaccatca cccagggctt
     9601 cgtgcacagt ccgccgttgc cgccggacga tttacggcgg tggatcgcga cccacgtgcc
     9661 caacggcaaa ccgagcgaac ccaagcaggc cgccgtcacg accgatcggt cagaacagtt
     9721 ctttggcgag tagctcgagc gtcgcgaccc gcgccggtgc ggtggccggg tcggtgcccc
     9781 ggtaggccga tgcaacgggg tgcaccatca cctcgtcgac gtcgaagcgt tgggcgagcg
     9841 cgcgcaactg ctcggcggcc tccgagggcg tgccgaggat ggcacgctgc agtccactgt
     9901 ccacgatgcg ctgttggtcc ggactgacgt gagccgcggc cgcctcctcg accagcggga
     9961 cgggtccgag cggctgcccg gtgcggagcc gcgccatcat ctgcaggttg ggcaacatca
    10021 gcgccgtcgc ctcgtcgctc gtttcggcca ccgcggcgtt gacggtcaag aacgtcacgg
    10081 gctcgggggt caggtcactc gggacgaacc gcgagcggta cagctccagc gcctcctcgg
    10141 tccccttgcc ggagaagtga tgggcgaaca cgtacggcaa ccccttggcg gcggccagat
    10201 tcgcggaata catcgatgag cccagtagcc acaggcgcgg ttcgcccacg gcggccggcg
    10261 tggccttgag ccggtagtcc cccgagcgca gcggaaccac cacgccacgc gcgctcatca
    10321 gcgcggctac gtcgtccaga tagttgggaa aattctcgat gtcgcggtcg tcgttggtgc
    10381 cgcgtagcgc gtacgacgtc accgggtctg acccgggcgc ccggccgatg cccaggtcga
    10441 tccggcccgg ggcggccgct tccagcagcg cgaactgctc tgcgaccgcc aacggcgcgt
    10501 gattgggcag catcacacca cccgagccca atcgcagctg cacggtctgc gccgccagat
    10561 aggcgatcag gaccggcggg ctggtggcgc cgacggacgg catgttgtgg tgttcggcga
    10621 cccagtagcg agtgaacccc agtcggtcgg cggtctgcgc cagctgcacg gtggcagcca
    10681 gcgcgtcgcg ggtgttctgg tcgctacgca ccgggacgag atcaaggacg gaaagatgca
    10741 cggaacactc aacgcctgac gccgcgcgaa ccttcccggc cgactcgccc ggtcagtagt
    10801 ccttcggggt gatcgagttc acgatgcggt cgaacacctc ctgcggaatc ggcgcaccac
    10861 cagggatgtt gtcgaccacc agccattgat tgcgcttgac gtagtcctgg aactctttgt
    10921 ggtagttggt gaagcccagc tcgtggtcgt cgccggctgc ggcgagctca cccgccagga
    10981 tgtaagcccc cagcagcgcg acgctggtcc cctggcccga caacggcgaa cagcaataag
    11041 cggcgtcgcc gaccagcgcc accctgccct ttgaccagcg atccatcttg atttgcgaca
    11101 tctcgtcgaa gtagaagtcc ggcgcggtcc gcatatatcg cagcagttgc gggcgaaccc
//
LOCUS       DASSGQ010000208         5732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_501525,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000208 DASSGQ010000000
VERSION     DASSGQ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501525"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..772)
                     /locus_tag="VFI55_09120"
     CDS             complement(47..772)
                     /locus_tag="VFI55_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7074223.1"
                     /translation="MERSSVDRQQRADRILDSARDLLLRWGYRRVTIDELAAHAGVGK
                     GTIYLHWRSREDVFHAVSAREAAAAMDAIVGALRSDPAEVALHRYLRRLFVEAMNRPV
                     LRALYTRDGDTLDKFLAAPNHQRLEETKLGVNRDYLGVLAAEGLLRSDLLPNDLDYTL
                     PVIVFGFFAAEPFLPPAIQLSLEQKADQLADTVRRAFEPASAPGKEARQRSMKRAAAK
                     AIPIFERLTRDYHAAAYGGDHND"
     gene            945..2564
                     /locus_tag="VFI55_09125"
     CDS             945..2564
                     /locus_tag="VFI55_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7074224.1"
                     /translation="MALFTNGDTANGIQVPVLIVGAGAAGLATSALLAKHGVRSLLVE
                     KRREVFIYPKARNLSFRSLEILRGLGLSDGVHKVAEQVSTMVVKPTLNSAEEKPAIDI
                     DAIFAGLDKLSPEPAAQYCPQSRLEPILLAETRRRGGEVRYGTELSSVDLDEAGVTVV
                     LHDRESGVSQTVRADYLVGADGVHSPIRKALKITTSGYGALPIYVVFIYFRAPWQKFV
                     ARLGEGDGVQVKNADVDGIFLVVEDDLGMFITTYFPGNGETADQFTPQRCREVLTAAI
                     GEPIDIDIIEVAAWQPYEQVADQFQCGRTFLVGDAAHTMPPFKAGGANTAIQSAHNLA
                     WKLAAVLKGTAGPELLATYHAERHPVGRFAARQSLTGPTVELLQLGGADRPQLPPDEE
                     CSMFALLIGYQYRSAAVVTGEPLDDPDAVHLVDELRAQPGTRVPHAWVRHRGERVSTL
                     DLVGPGFTVLTRDERWRAAVTSASAALDIPIAVHCIGDDGWAAVTGLGPEGALLVRPD
                     DFVGWRADELPADPESQLRQALSAILSRSPR"
     gene            complement(2566..3987)
                     /locus_tag="VFI55_09130"
     CDS             complement(2566..3987)
                     /locus_tag="VFI55_09130"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HET7074225.1"
                     /translation="MTIMAPASVGESLDPRDPLLRLCTFFDNDSVELLHERDRSGVLA
                     ASGTVNGLRTIAFCTDGTVMGGAMGIEGCTHIVNAYDTAIEEQSPIVGIWHSGGARLA
                     EGVRALHAVGLVFEAMIRASGYIPQVSVVVGFAAGGAAYGPALTDVVVMAPESRVFVT
                     GPDVVRSVTGEDVDMASLGGPETHHKKSGVCHIVADDELDAYERGRRLVGLFCQQGHF
                     DRSKAEAGDTDIHALLPESARRAYDVHPIVTALLDEDIPFDEFQANWAPSMVVGLGRL
                     SGRTVGVLANNPLRLGGCLNSESAEKAARFVRLCDAFGIPLIVVVDVPGYLPGVDQEW
                     GGVVRRGAKLLHAFGEATVPRVTLVTRKTYGGAYIAMNSRSLNATKVFAWPDAEVAVM
                     GAKAAVGILHKKKLAAAPDHEREALHDDLAAEHERIAGGVDSAIEIGVVDEKIDPAHT
                     RSKLTEALAQAPARRGRHKNIPL"
     gene            complement(4033..5286)
                     /gene="kasB"
                     /locus_tag="VFI55_09135"
     CDS             complement(4033..5286)
                     /gene="kasB"
                     /locus_tag="VFI55_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003411576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase KasB"
                     /protein_id="HET7074226.1"
                     /translation="MTELVTGKALPNVVVTGIAMTTALATDAETTWKLLLDSQSGIRT
                     LDDPFVEEYNLPVRIGGHLLEEFDHQLTRVELRRMGYLQRMSTVLSRRLWENAGNPEL
                     DTNRLAVSIGTGLGSAEELVFGYDEMRVRGYRAISPLAVQKYMPNGAAAAVGLERHAK
                     AGVMTPISACASGSEGIARAWQQIVLGEADAAICGGVETRIEAVPIAGFSNMRIVMST
                     NNENPAGACRPFDKDRDGFVFGEAGALMLIETEESAKARGANILGRIMGASITSDGFH
                     MVAPDPNGERAGHAITRAVQLAGLTPGDIDHVNAHATGTQVGDVAEGKAINNALGSNK
                     PAVYAPKSALGHSVGAVGAVESILTVLALREQVIPPTLNLENLDPEIDLDVVAGKPRP
                     GNYQYAINNSFGFGGHNVAIAFGRY"
     gene            complement(5314..>5732)
                     /locus_tag="VFI55_09140"
     CDS             complement(5314..>5732)
                     /locus_tag="VFI55_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216761.1"
                     /note="FabF; beta-ketoacyl-ACP synthase II, KASII;
                     catalyzes a condensation reaction in fatty acid
                     biosynthesis: addition of an acyl acceptor of two carbons
                     from malonyl-ACP; required for the elongation of
                     short-chain unsaturated acyl-ACP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HET7074227.1"
                     /translation="APAPDGICAGRAMTRSLELAGLSPADVDHVNAHGTATPIGDTAE
                     ANALRVAGCEQAAVYAPKSALGHSIGAVGALESILTVLALRDGVIPPTLNYETPDPEI
                     DLDVVAGEPRYGDFRYAINNSFGFGGHNVALAFGRY"
BASE COUNT          957 a         1885 c         1871 g         1019 t
ORIGIN      
        1 gtccaccccg tactgcccgc gcgggcgggc ggggtcagaa gccggctcag tcattgtgat
       61 cgcctccata cgccgcggcg tggtagtccc gggtaagccg ttcgaaaatt gggatcgctt
      121 tcgccgcagc gcgtttcata gatcgttgac gagcctcctt gccgggcgcg ctggccggct
      181 cgaacgcgcg gcgcacggtg tccgcgagtt ggtcggcctt ctgctccagg ctcaactgga
      241 ttgccggcgg caaaaacggc tcggctgcga agaagccgaa gacgatgacg ggcaaggtgt
      301 aatcgagatc attcggcagc aagtcggatc gcagcagtcc ctccgcggcc aaaacaccca
      361 ggtagtcgcg gttgacgccc agcttggttt cctccagccg ctgatgattc ggcgcggcca
      421 ggaacttgtc gagcgtgtcg ccatcgcgtg tgtagagcgc gcgcaagacg gggcggttca
      481 tcgcctcgac gaatagccgg cgcaggtagc ggtgcagcgc gacctcggcg ggatcacttc
      541 gcagcgcacc gacaatggcg tccatggccg ccgccgcctc tctggcgctc acggcgtgaa
      601 agacatcctc ccgagatcgc cagtgcaggt agatggtgcc cttgccgaca ccggcgtgag
      661 cggccaattc atcgattgtc acccgccgat agccccagcg gagcagcagg tcacgcgcgc
      721 tatccaggat ccgatcggcc cgctgttgcc ggtccacgct cgaacgctcc acgatcaccg
      781 ccgctctccc ggatgcccgt gaccaaaata ccatatcagt catcagtcac aagggggttg
      841 gcgagggctg gcggttggca attactggaa gtaactattg tttgatagtt acttccagta
      901 tgagtaatat ggtgctgtcg accccacgaa agctggtgga aactatggcg ttatttacga
      961 acggtgacac tgccaacggc atccaggtac ccgtgctcat cgtcggcgcc ggcgccgccg
     1021 ggctggcgac gtcggcattg ctggccaagc acggtgttcg ttcactgctg gtcgagaagc
     1081 gacgcgaggt gttcatctat ccgaaggccc gcaatctgag cttccgcagc ttggaaattc
     1141 tgcgcggcct cggtctcagc gacggggtcc acaaggtcgc cgagcaggtg tcgaccatgg
     1201 tcgtcaagcc gacgctgaat agcgcggagg aaaagccggc catcgacatc gacgcgattt
     1261 tcgcgggcct ggacaagctc agccccgagc ccgccgcgca gtactgtccc caaagcaggc
     1321 tcgagccgat cctgctcgcc gaaacacgcc ggcgcggtgg cgaagtgcgc tacgggacgg
     1381 aactctcctc agtggatctc gacgaggccg gtgtcaccgt ggtgctgcac gaccgggagt
     1441 cgggtgtatc ccagactgtc cgcgccgact atctcgtcgg cgccgacggc gtgcacagcc
     1501 cgatccgtaa agcgctgaag atcacgacat ccgggtacgg cgcgctgccg atctatgtcg
     1561 tcttcatcta cttccgggcg ccttggcaga agtttgttgc gcggttgggc gaaggcgacg
     1621 gggttcaggt caaaaacgct gacgtggatg gcatattcct cgtggtcgaa gacgacctgg
     1681 gaatgttcat caccacgtat ttccccggca acggtgagac ggcggaccag ttcacgccgc
     1741 aacggtgccg cgaggtgctc accgcggcca tcggcgagcc gatcgatatc gacatcatcg
     1801 aggtcgcggc ttggcaaccc tacgaacagg tggccgatca attccagtgt gggcggacct
     1861 ttctcgttgg cgacgcggcg cacaccatgc cgccgttcaa ggctggggga gccaacaccg
     1921 cgatccagag tgcgcacaac ctggcctgga agcttgccgc cgtgctcaag gggacggcgg
     1981 gaccggaact gcttgccacg taccacgccg agcgtcaccc ggtggggcgc ttcgccgctc
     2041 gtcagtccct caccggtccg acggttgaat tgctgcagtt gggcggcgct gatcggccac
     2101 agttgccgcc cgacgaagag tgttcgatgt tcgcgctgct catcggctac caatatcgtt
     2161 cggcggccgt ggtgaccggc gagcctttgg acgatccgga tgcggtgcac ctcgtcgacg
     2221 agttgcgcgc tcagccgggc acccgggttc cacacgcctg ggtgcggcac cgcggcgagc
     2281 gtgtttcgac gctggacctc gtcggtccgg ggttcacggt gcttacccgg gacgaacgat
     2341 ggcgcgcggc ggtaacttcg gcgtcggccg ccctggacat cccgatcgcc gtgcattgca
     2401 tcggcgacga cggatgggcg gctgtcaccg gactcgggcc tgagggggcg ctgctggtcc
     2461 ggccggacga cttcgtcggc tggcgtgccg acgaactgcc cgccgatccc gaaagtcagc
     2521 tgcgtcaagc gctttcggcg atattgtctc gcagcccgcg ctagatcaga gcgggatgtt
     2581 cttgtggcgg ccgcggcgtg cgggcgcctg ggcaagcgct tcggtgagct tgctgcgagt
     2641 gtgcgccggg tcgatcttct cgtcgaccac accgatctcg atggcactgt cgacgccacc
     2701 ggcaatgcgc tcgtgctcgg cagccagatc gtcgtgtagc gcttcgcgct cgtggtcagg
     2761 cgcggcggca agcttcttct tgtgcaggat gccgacggcg gctttggcgc ccatcacggc
     2821 gacctcggcg tccggccacg cgaacacttt ggtggcgttc agcgagcggg agttcatcgc
     2881 aatgtaggcc ccgccgtacg tctttcgggt gaccagcgtg actcgcggaa cggtggcctc
     2941 accgaacgcg tgcaacagtt tggcgccgcg gcgcaccacg ccgccccact cctggtcgac
     3001 accgggcagg tagccgggga catcgacgac cacaatcagc gggatcccaa acgcgtcgca
     3061 cagtcgcacg aaacgcgctg ccttctcggc gctttcggag ttcaggcagc cgcccagtcg
     3121 cagcgggttg ttggccagca cgccgaccgt gcggccggac aggcgaccca ggccgacaac
     3181 catcgacggc gcccaattgg cctggaactc gtcgaacggg atgtcctcat cgaggagcgc
     3241 ggtcacgatc gggtgcacgt cgtaggcccg tcgagcggat tcgggcagca acgcgtggat
     3301 gtcggtgtca ccggcctcgg ccttgctgcg gtcgaaatgc ccttgctggc agaataatcc
     3361 gaccaaccgg cgaccgcgct cgtaggcatc gagctcgtcg tcggcgacga tatggcacac
     3421 cccggacttc ttgtggtggg tctccgggcc accgagcgag gccatgtcga catcctcgcc
     3481 cgtgacgctg cgcaccacgt cgggtccggt gacgaacacc cggctctccg gcgccatgac
     3541 gaccacgtcg gtcagcgccg gtccgtaggc ggcgccaccg gcggcgaaac cgacgaccac
     3601 ggagacctgc ggaatgtagc cggaagcgcg aatcatcgcc tcgaacacca ggccgaccgc
     3661 gtgcagcgct cggacgcctt cggcgagccg cgcaccaccc gagtgccaga tgcccacgat
     3721 cgggctctgc tcctcgatgg cggtgtcgta ggcgttgacg atgtgggtgc acccttcgat
     3781 gcccatggct ccgcccatca ccgttccgtc tgtgcagaac gcgatagtgc ggaggccgtt
     3841 cacggtgccc gacgcggcca gcactccgga gcggtcacgc tcgtgcagca gttccacgct
     3901 gtcgttgtcg aagaacgtgc acaaacgcag cagcggatcg cgagggtcaa gcgattcgcc
     3961 aaccgacgcg ggggccatga ttgtcatcgc gggtctcctg tttccggtga agccatgatt
     4021 gctcgggggt tttcagtacc gcccaaacgc gatcgccacg ttgtgaccgc cgaatccgaa
     4081 cgaattgttg atcgcgtact ggtaattgcc cggccgcggc ttacccgcta ccacgtccaa
     4141 gtcgatctct ggatcgaggt tttcgagatt cagtgtcggc ggaatcacct gctcccgcaa
     4201 cgcaagtacg gtcaggatcg actccaccgc gccgaccgca cccaccgagt ggcccagcgc
     4261 ggatttgggc gcatacaccg ctggcttgtt gctacccagc gcgttgttga tggccttgcc
     4321 ctcggccacg tcaccgacct gggtaccggt ggcgtgggcg ttgacgtgat cgatgtcgcc
     4381 cggggtgagg cccgcgagct ggaccgcccg cgtgatcgcg tgcccggcgc gttcgccgtt
     4441 ggggtccggt gccaccatgt ggaagccgtc cgaggtgatg ctggccccca ttatccggcc
     4501 caggatgttg gcgccacgag cctttgcgct ctcctcggtt tcgatcaaca tgagggcgcc
     4561 ggcctcgccg aacacgaagc cgtcgcggtc cttgtcgaac gggcgacacg cgccggcggg
     4621 gttttcgttg ttggtggaca tcacgatgcg catattggag aacccggcga tgggcaccgc
     4681 ctcgatcctg gtttccacgc caccgcagat ggccgcgtcg gcctcaccga gcacgatctg
     4741 ctgccacgcg cgggcgatgc cctccgaacc ggacgcacac gccgatatcg gcgtcatcac
     4801 gccggccttc gcgtgccgtt ccagcccgac cgccgcggcg gcgccgttgg gcatgtactt
     4861 ctgcaccgcc agcggggaga tcgcccggta gccgcgcaca cgcatctcgt catatccgaa
     4921 gacgagctct tcggcggagc ccagaccggt accgatggac accgccaacc ggttggtgtc
     4981 gagctcgggg ttgccggcgt tctcccacag gcgccggctc agaaccgtgg acatgcgctg
     5041 caggtaaccc atccgacgca gctcaacgcg cgtgagctga tgatcgaact cctccagcag
     5101 atgccccccg atgcgcacgg ggaggttgta ctcctcgacg aacggatcgt cgagcgtgcg
     5161 gatgccgctt tggctgtcga gcagcagctt ccacgtcgtt tccgcgtcgg tcgccagcgc
     5221 ggttgtcatg gcgatgccgg tgacaaccac gttgggtaac gccttacccg taaccagctc
     5281 tgtcatgcgt cttgctttcg ttccttccgt gcttcagtaa cgcccaaagg cgagcgccac
     5341 gttgtgaccg ccgaacccga acgagttgtt gattgcataa cgaaaatcgc cgtagcgagg
     5401 ttcgcccgcg acaacatcga ggtcgatctc gggatccggg gtctcgtagt tcagtgtggg
     5461 cggaatgact ccgtccctaa gggccaacac cgtgaggatg gattcaagcg cgcccaccgc
     5521 accgatggag tggcccagtg ccgacttcgg cgcgtatacc gcggcctgtt cgcaaccggc
     5581 gacgcggagg gcgttggcct cggcggtgtc gccgatcggc gtcgccgtcc cgtgcgcgtt
     5641 gacgtggtcg acgtccgccg gcgacaagcc cgccagctcc agcgaccgcg tcatcgccct
     5701 gccggcacag atgccgtccg gcgcgggcgc ca
//
LOCUS       DASSGQ010000209         5444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1351961,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000209 DASSGQ010000000
VERSION     DASSGQ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351961"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2703)
                     /gene="car"
                     /locus_tag="VFI55_09145"
     CDS             complement(<1..2703)
                     /gene="car"
                     /locus_tag="VFI55_09145"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019305838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylic acid reductase"
                     /protein_id="HET7074228.1"
                     /translation="MSTATQEERLARRVDDLTAKDPQFAAARPDPAVAEAIGQPGLRL
                     PQIIQTTLDGYAQRPALAQRAVEVVKDAKTGRTSLELLPRFETVSYGELGERAGALAR
                     ALAHDVVHTGDRVCVLGFNSVDYATIDIALGLAGAVSVPLQTSAAITQLQPIVAETEP
                     TLIAASVNQLPDAVELIRTADPRPARLVVFDYHPEVDDEREALEKARETANARPADAA
                     VIVETLADVVARGKTVSSGPARPAATTIDDAQSLALLIYTSGSTGAPKGAMYPQSNVG
                     KMWQRSDKNWFGPSAASITLNFMPMSHVMGRGILYGTLGNGGTAYFTARSDLSTLLED
                     LELVRPTELNFVPRIWETLFGEFQRQVDTRLAAATDPSDPTAHAAVEAEVMDELREYQ
                     LGGRFIFAMTGSAPTSPELKEWVESLLDLHLLDGYGSTEAGMVLFDGEVQRPPVIDYK
                     LVDVPDLGYFATDRPYPRGELLLKTANMFPGYYKRPEVTASVFDPDGYYRTGDVVAET
                     APNRLVYVDRRNNVLKLAQGEFVTVAKLEAVFGNSPLVRQIYIYGNSAHPYLLAVVVP
                     TEEALARYADGGLDALKGPIADSLQDVAKEAGLQSYEVPRDFLIETTPFSLENGLLTG
                     IRKLAWPKLKQHYGERLEQLYTELADSQANELRALRQNGADAPVLQTVSRAAAAMLGT
                     ATTELTADAHFTDLGGDSLSALTFGNLLHEIFDIEVPVGVIVSPANDLAAIADYIEAE
                     RQGNKRPSFAAVHGREATEVHASDLTLDKFIDASTLAAAPDLPKPSAEVRTVLLTGAT
                     GFLGRYLALDWLERMDLVDGKVIALVRAKSDAEGRARLDRIFDSGDPKLLDHYRRLAA
                     DHLEVIAGDKGEANLGLDQQTWQRLADTVDLIVDPAALVNHVLPY"
     gene            complement(2860..4200)
                     /gene="gabT"
                     /locus_tag="VFI55_09150"
     CDS             complement(2860..4200)
                     /gene="gabT"
                     /locus_tag="VFI55_09150"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HET7074229.1"
                     /translation="MAGLEQTRQLVTEIPGPVSLELNKRRAAAVSHSVNITLPVFVAR
                     AGGGIVEDVDGNRLIDLGSGIAVTTIGNSSPRVVDAVREQVADFTHTCFMVTPYEEYV
                     AVAEEVNRITPGSGDKRSVLFNSGAEAVENAVKVARAYTRKPAVVAFDHAYHGRTNLT
                     MALTAKSMPYKSGFGPFAPEIYRAPLSYPYRDGLLDKELATDGEKAAARTIRVIDSQV
                     GADSLSAIVIEPIQGEGGFIVPAEGFLPTLLDWCHKNGVLFIADEVQTGFARSGAMFA
                     CEHEGIEPDLICTAKGIADGLPLSALTGSAEIMDAPHVGGLGGTFGGNPVACAAALAT
                     IATIENDGLIERAQQIERVITERLLRLQAGDDRIGDVRGRGAMIAVELVKTGTADPDA
                     ALTSALATALHAAGVIVLTCGMFGNVIRLLPPLTISDELLIEGIDILSLILRDL"
     gene            4397..4825
                     /gene="yajC"
                     /locus_tag="VFI55_09155"
     CDS             4397..4825
                     /gene="yajC"
                     /locus_tag="VFI55_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET7074230.1"
                     /translation="MESLVLFLPFLLIMGGFMYFASRRQKRAMQATIDLHESLRPGDR
                     VHTTSGMEATVVGITDDTVDLEIAPGVRTTWMKLAIRDRILPDDADEDVDLDDDYDDE
                     DADEDPDDDLDEHAAGELEGSHEPNRSAHPTDETRVIKDS"
     gene            4918..>5444
                     /locus_tag="VFI55_09160"
     CDS             4918..>5444
                     /locus_tag="VFI55_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET7074231.1"
                     /translation="MASSSAPVHPARYLSVFLLLLIGVYLLVFLTGDKRAAPKLGIDL
                     RGGTRVTLTARTPDGSRPSREALAQAQQIISARVNGLGVSGSEVVVDGDNLVITVPGN
                     DGSEARNLGQTARLYIRPVLNSVPAQGAEPKPAPPPAGAPPAAPAGPGGPAPGQPPPP
                     GAPAPPGQPAPPSQPG"
BASE COUNT          853 a         1818 c         1868 g          905 t
ORIGIN      
        1 gtagggcagg acgtggttga ccagggcggc ggggtcgacg atcaggtcga cggtgtcggc
       61 cagccgttgc caggtctgct ggtccaggcc gaggttggcc tcgcccttgt cgccggcgat
      121 gacctccagg tggtcggcgg ccagccgccg gtagtggtcc agcagcttgg ggtcgccgct
      181 atcgaaaatt cggtccagac gcgcacgccc ctcagcgtcg ctcttggccc taaccagcgc
      241 gatcaccttg ccgtcgacca ggtccatccg ctccagccag tccagggcca ggtagcggcc
      301 caggaagccg gtggcgccgg tcagcaggac ggtgcgcacc tcggcactgg gcttcggcag
      361 gtcgggcgcg gcggccaggg tcgacgcatc gatgaacttg tccagcgtga gatcgctggc
      421 gtgcacctcg gtcgcttcgc ggccgtgaac ggcggcgaag ctgggccgct tgttcccctg
      481 ccgttcggcc tcgatgtagt cggcgatggc cgccaggtcg ttggccgggc tgacgatgac
      541 gcccaccggc acctcgatgt cgaagatctc gtgcaggagg ttgccgaatg tcaacgccga
      601 cagcgagtct ccgcccaggt cggtgaagtg ggcatcggct gtcaactcgg tggtggccgt
      661 ccccagcatc gcggccgcgg cccggctcac tgtctgcagc accggcgcgt cggccccgtt
      721 ttgacgaagc gcgcgcagtt cgttggcctg gctgtcggcc agctcggtgt agagctgttc
      781 cagtcgctcg ccgtagtgct gcttcagctt cggccacgcc agcttgcgga tgccggtcag
      841 caggccgttc tccaggctga acggcgtcgt ctcgatgaga aagtcgcgcg gcacctcgta
      901 ggactgcaag ccggcctctt tcgcaacgtc ctgcagcgaa tcggcgatcg gtcccttcag
      961 cgcgtcgagc ccaccgtcgg cgtagcgcgc cagggcctct tcggtgggca ccacgacggc
     1021 gagcaggtag gggtgcgcgc tgttgccgta gatgtagatc tgccggacca gcgggctatt
     1081 gccgaacacc gcctccagct ttgccacggt gacgaactcg ccctgcgcga gcttgagcac
     1141 gttgttacgg cggtcgacgt acaccaggcg gttgggagcg gtctcggcga cgacgtcacc
     1201 ggtccggtag tagccgtcgg ggtcgaacac gctcgcggtg acctcgggcc gcttgtagta
     1261 gccggggaac atgttcgccg tcttgaggag caactcgccg cgcgggtacg gccggtcagt
     1321 ggcgaaatag cccagatccg ggacgtcgac cagcttgtag tcgatcaccg gcggacgttg
     1381 cacttcaccg tcgaacagga ccatcccggc ctcggtggag ccgtagccgt ccagcagatg
     1441 caagtcgagc agggattcca cccactcctt cagctccgga gacgtgggcg ccgagcccgt
     1501 catcgcgaaa atgaaccgcc cgcccagctg gtactcgcgc agctcgtcca tgacctcggc
     1561 ctcgacggct gcgtgggccg tggggtcgct cggatcggtg gctgccgcga gccgggtgtc
     1621 gacctggcgc tggaattcgc cgaacagggt ctcccagatc cgcggcacga agttcagctc
     1681 agtgggcctg accagctcga ggtcctcgag caacgtcgag aggtcactgc gcgcagtgaa
     1741 gtatgcggtg ccgccgtttc cgagagtgcc gtagaggatg ccgcggccca tcacgtggct
     1801 catcggcatg aagttgaggg tgatcgacgc tgcgctcggc ccgaaccagt tcttgtccga
     1861 ccgttgccac atcttgccga cgttgctttg ggggtacatc gcacccttgg gtgcgccggt
     1921 gctgccggag gtgtagatca gcagagccag cgattgcgca tcgtcgatcg tcgtggccgc
     1981 gggccgggcc ggccccgaag acacggtctt tccgcgcgcg acgacgtcgg ccagggtctc
     2041 gacaatcacc gcggcgtcgg ccggccgcgc attggcggtc tcgcgggcct tctcaagggc
     2101 ctcgcgctcg tcgtcgacct cggggtggta gtcgaacacc acgagcctgg cgggccgcgg
     2161 gtcggcggtg cggatcagct cgaccgcgtc gggcagctga ttcacgctcg ccgcaatcag
     2221 ggtgggctcg gtttcggcga cgatcggttg cagctgggtg atcgcggcgc tggtctgcag
     2281 cggaacggac acggcgccgg ccaggcccag tgctatgtcg atggtggcgt agtcgacgct
     2341 gttgaagccc aacacgcaca cccggtcacc ggtgtgcacc acatcgtggg ccagggcacg
     2401 tgccagcgcg ccagcgcgct cgcccagctc gccgtagctg acggtctcga agcgggggag
     2461 cagttcgagc gaggtacgtc cggtcttcgc gtctttgacg acctcgaccg cgcgctgggc
     2521 cagcgccggc cgctgcgcgt aaccgtcgag cgtggtctgg atgatctggg gcagtcgcag
     2581 cccgggctgg ccgatggcct cggcgacggc agggtccggc cgcgcggcgg cgaactgtgg
     2641 gtccttggcg gtgaggtcgt cgacgcggcg tgcgagccgc tcttcctgag tagcagtcga
     2701 catagcgagt cctcttatta agcaaaaagc cgaatattcg aaagcgcgac gctgcgctgc
     2761 cgaataaaac gttagcgaaa ctaacgggta ttccgcaatg accggcgcgg agggtgtgag
     2821 attccacacc cggcgttgcg gtgatcgggg tcccggactt tacaggtcgc gcaggatcag
     2881 actcaggata tcgattccct cgatgagcaa ctcatcgctg atggtcagcg ggggcagcag
     2941 tcggatgacg ttgccgaaca tgccgcaggt caacacgatc accccggccg cgtggagcgc
     3001 ggtggcaagc gccgaggtca atgcggcgtc ggggtcggcc gtgcccgtct tgaccaactc
     3061 cacggcgatc atggcaccgc ggccacggac gtcaccgatc cggtcgtcgc ccgcctgcag
     3121 ccgcagcagc cgctcggtga tcaccctctc gatctgctgt gcccgctcga tcaggccgtc
     3181 gttctcgatg gtcgcgatgg tggccagcgc cgccgcgcac gcgacggggt taccgccgaa
     3241 ggtgccgccc agtccgccga cgtgcggggc gtccatgatc tcagcactgc cggtcagcgc
     3301 cgacagcggc aatccgtcgg cgatgccttt ggcggtacag atcagatccg gctcgatccc
     3361 ttcgtgctcg caggcgaaca tcgcgccgga gcgcgcaaac ccggtctgca cctcgtcggc
     3421 gatgaacagc acgccgttct tgtggcacca gtcgagcagg gtgggcagaa agccctcggc
     3481 gggaacgatg aatccgcctt cgccctggat gggttcgatg acgatcgcgg acaggctgtc
     3541 ggcgccgacc tggctgtcga tgaccctgat ggtccgcgcg gcggcctttt cgccgtccgt
     3601 ggccagctct ttgtcgagca gcccgtcgcg gtacgggtag gacagcggcg cgcgatagat
     3661 ctccggcgcg aacgggccga agccgctctt gtagggcatc gacttggcgg tcagtgccat
     3721 cgtcaggttg gtgcggccgt ggtaggcgtg gtcgaacgcc accaccgcgg gcttgcgcgt
     3781 gtaggcgcgc gccaccttga cggcgttctc gaccgcttcg gcgcccgagt tgaacagcac
     3841 cgagcgttta tcgccggaac ccggggtgat cctgttgacc tcttcggcga cggcgacgta
     3901 ctcctcgtac ggcgtcacca tgaaacaggt gtgggtgaag tcggccacct gttcgcgcac
     3961 cgcgtccacc acccggggcg acgaattgcc gatcgtggtg accgcgatgc cggaacccag
     4021 gtcgatgagc cggtttccgt ccacgtcctc gacgatgccg ccgccggcgc gcgccacgaa
     4081 caccggcaag gtgatgttga cgctgtggga taccgcggcg gcccggcgct tgttcagctc
     4141 cagcgacacg gggccgggaa tttcggtgac cagctggcga gtctgctcga gcccggccac
     4201 gtcatcctcc tcaccgcgcg cgcctgtcgc gtcgcgggat aaagcctatc tggtcagcca
     4261 cgagtcggcg gctcgagcgg cgggcgaccg gtcacccgcg cacactgccc gtcagtcgcg
     4321 acctgcggtg ctggcagact ggccgttacg aggtgcgtat tcagcgcaat attcgactgc
     4381 gaaagacagg cgcgcgatgg agagtttggt cttgttcttg ccgttcctgc tcattatggg
     4441 cggcttcatg tacttcgcgt cgcgtcgcca aaagcgggcg atgcaggcca ccatcgacct
     4501 gcacgagtcg ttgcggcccg gcgaccgggt gcacaccact tccgggatgg aagccaccgt
     4561 cgtcgggatc accgatgaca ccgtcgacct cgagatcgcg ccgggcgtgc gcaccacgtg
     4621 gatgaagttg gccattcgcg accggatcct gccggacgac gcggacgagg acgtggacct
     4681 cgacgacgac tacgacgacg aagacgccga cgaggacccg gacgacgacc tcgacgaaca
     4741 cgccgccggc gagttggaag gctcccatga gcccaaccgt tcggcccacc ccaccgacga
     4801 gacgcgggtg atcaaggatt cctgagcgcg acccgcgacc gctcgcgcaa gggcaataga
     4861 ggccggcacg taggctctgt cgggtaagaa accgattctc aaggagatac aaggaacgtg
     4921 gcatcgtctt ctgcgccggt gcaccctgcc cgctacctgt cggtcttctt gttattgctc
     4981 atcggcgtct acctgctggt gtttctcacc ggagacaagc gagccgctcc caagctcggc
     5041 atcgacctgc ggggcggtac ccgcgtcacg ctgaccgccc ggaccccgga cggctcgcgg
     5101 ccgagccggg aggcgctggc ccaggcgcag cagatcatca gcgcgcgcgt gaacgggctc
     5161 ggtgtgtccg gttcggaggt cgttgtcgac ggcgacaacc tggtcatcac cgtgcccgga
     5221 aacgacggca gcgaggcccg caacctggga cagaccgcgc ggctgtacat ccggcccgtg
     5281 ctcaactcgg tgcctgcgca gggcgccgag cccaagccgg cacccccgcc cgccggcgcc
     5341 ccgcccgcgg cccctgccgg acccggcggg cccgcgccgg ggcaacctcc gccaccggga
     5401 gcacccgcac cgcccggtca acccgcaccc cccagccaac cggg
//
LOCUS       DASSGQ010000210        10755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_318744,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000210 DASSGQ010000000
VERSION     DASSGQ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10755
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318744"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="VFI55_09165"
     CDS             complement(<1..1277)
                     /locus_tag="VFI55_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HET7074232.1"
                     /translation="MSFLIAAPQLIADAATDLANIGSTIGSANFAAATATTGVLPAAA
                     DEVSAQIAALFSQHAAGYQQLSAQAALFHEQFVQAMTSGASAYAAAEANVVQTLAPAL
                     AVDLSGGLSGLGASLSAGVSEMGASFDAMLSGSLGGSFSGFAPVGATLAADFGGGLSA
                     LGQAGGALSSSFGAGLSGLAGGFSAGLPGFGGSLTGGLSGLSTVLSGGFSGSFSGGLS
                     GLGAGLSGFLQTGASLVGNLGGSLPGLGVSLPVSLNAGLAAFGSLFEVTPSFGVNFAT
                     GLSGLGAQLSAALNGGLAVGIAGLPTLLSYGVSPFQALVTAGSQTGFFNQLQAMELGF
                     NTALVDGEFGFNNSLVAQETALETAIFGGTGAWNGVIDNVFNFWNSVLGTGEVTFDTL
                     LGAQIPSYVAPISWYDLATSLYVGPMSNVIGGG"
     gene            complement(1539..3422)
                     /locus_tag="VFI55_09170"
     CDS             complement(1539..3422)
                     /locus_tag="VFI55_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter family substrate-binding
                     protein"
                     /protein_id="HET7074233.1"
                     /translation="MSVPTRTRRLFLAVGALVSLVGVMLAACTVNPPPAPQSTDTPHS
                     SPPPPQRPAQIIVGIDSIGAGFNPHLLSDLSPVNAAISALVLPSAFRPVPDPNTPTGS
                     RWEMDPTLLVSAEVTNQNPFTVSYKIRPEAQWTDNAPIGADDFWYLWRQMVGQPGVVD
                     PAGYDLITGVQSLEGGKQAVVTFSQPYPAWKELFSNIVPAHIVKDVPGGFAAGLARAL
                     PVTGGQFRVESIDPQRDEILIARNDRYWGAPAKPALILFRRAGAPAALADSVRNGDTQ
                     VAQVHGGSAAFAQLSAIPDVRTARIVTPRVMQLTLRANEPKLSDTQVRKGILGLLDVD
                     LLAAVGAGSDNTVTLDQAQIRSPSDPGYEPTAPAAMTTQAALALLAASGYQVEPNASA
                     SPAPTPANTGPPEVIRGRISKDGQQLALVIGVAVNDPTSVAVANTAADQLRNVGIAAT
                     VLALDPVALYRDALNNNQVDAIVGWHQAGGNLATLLASRYGCPALQATQVPTAVTPTT
                     TPAAASPPAAPGTTSLTPTQAPPSRPTEPGALVQAPSNLTGICDRSIQSNIDAALNGS
                     KSINDVITAVEPRLWNMSTVLPILQDTTIVAAGPSVQNVSLSGAVPVGIVGDAGQWIK
                     TGS"
     gene            complement(3547..5436)
                     /gene="typA"
                     /locus_tag="VFI55_09175"
     CDS             complement(3547..5436)
                     /gene="typA"
                     /locus_tag="VFI55_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET7074234.1"
                     /translation="MPFRNVAIVAHVDHGKTTLVDAMLRQSGALTHRGDDTQERIMDS
                     GDLEKEKGITILAKNTAVHRHDPDGTVTVINVIDTPGHADFGGEVERGLSMVDGVLLL
                     VDASEGPLPQTRFVLRKALAAHLPVILVVNKTDRPDARIAEVVEASHDLLLDVASDLD
                     DEAAAAAEHALGLPTLYASGRVGVASTTQPLDGHVPDGANLDPLFEILEKYVPAPKGD
                     PEAPLQALVTNLDASTFLGRLALIRIYNGKIRKGQQVAWMREVDGLPVITSAKITELL
                     ATEGVERSATAEAIAGDIVAVAGLPEIMIGDTLADPDHAHALPRITVDEPAISVTIGT
                     NTSPLAGKVSGHKLTARMVRSRLDAELVGNVSIRVVDLGRPDAWEVQGRGELALAVLV
                     ETMRREGFELTVGKPQVVTRTIDGQLHEPFEAMTIDCPEEFVGAVTQLMAGRKGRMEE
                     MANHAAGWVRMDFIVPSRGLIGFRTDFLTLTRGTGIANAVFDGYRPWAGEIRARHTGS
                     LVSDRSGAITPFAMIQLADRGQFFVEPGQDTYEGMVVGINPRAEDLDVNITREKKLTN
                     MRSSTADVIETLARPLELDLEQAMEFCAADECVEVTPEIVRVRKVELEATTRARSRAR
                     AKARG"
     gene            complement(5575..5967)
                     /locus_tag="VFI55_09180"
     CDS             complement(5575..5967)
                     /locus_tag="VFI55_09180"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HET7074235.1"
                     /translation="MPTQIVVAGAVIAGSALLVAQRVRPPELAGRWELPGGKVAPGET
                     ERDALARELAEELGLEIADIVVGDRLGVDIPLNATATLRAYRVRLISGEPHPHDHRAL
                     RWVTAAELDDIDWVPADRGWLEDLAGVL"
     gene            6096..6365
                     /locus_tag="VFI55_09185"
     CDS             6096..6365
                     /locus_tag="VFI55_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074236.1"
                     /translation="MSPFNTILRNGAAFEAQLRPILEEYCVALDLEGCFDGLSASEAH
                     QAAVAILVAVCGRDDPADHLRQSADDMVANALAISRSLRIAAAVW"
     gene            6449..6649
                     /locus_tag="VFI55_09190"
     CDS             6449..6649
                     /locus_tag="VFI55_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HET7074237.1"
                     /translation="MAAKFEISKDKSGKFRFHLKAANGEIITASQGYETKASAQKGIE
                     SVKTNAPGAAVVHLTEREPAHN"
     gene            6982..7266
                     /locus_tag="VFI55_09195"
     CDS             6982..7266
                     /locus_tag="VFI55_09195"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HET7074238.1"
                     /translation="MAVLTDEEVDAALPELNGWQRADGALRRSIKFPTFIAGIDAVRR
                     VAEHAESKDHHPDIDIRWRTVTFTLVTHSAGGITKNDIAMARDINGIVGS"
     gene            complement(7263..8594)
                     /locus_tag="VFI55_09200"
     CDS             complement(7263..8594)
                     /locus_tag="VFI55_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase"
                     /protein_id="HET7074239.1"
                     /translation="MLQTAVTTDNATVSVAIAKVGQIETTSAAQAAPFTRRLASRLQA
                     TAPALLILSVLARLAWSYLMPNGANFVDLHVYLGGAAAIDHPGTLYSYVYADQTPDFP
                     LPFTYPPFAAVVFYPLHLLPFGLVAFLWQIATMAALYGAVRISQRLIGVAAGAGQRVA
                     MLWTAITIWIEPLRSTFDYGQINVLLMLGVLWAVYTTRWWLSGLLVGVAAGIKLTPAI
                     SGVYFVGARRWAAAVFSALVFVATVGVSVLVVGDQTRYYFTELLGDAHRVGPIATSFN
                     QTWRGGISRILGHDAGFGPPVLAAIAVTAVLAVLAWRALDGPDQLGKLLVVELFGLLL
                     SPISWTHHWVWVVPLMIWAIHGPPRCRPGAPIVGWGWLALTIIGVPWLLSFAQPTIWQ
                     ISRPWYLAWAGLVYVVAAVATLIWIAVTGRRDTGPDLPAPQRRRVRAASED"
     gene            8766..9551
                     /locus_tag="VFI55_09205"
     CDS             8766..9551
                     /locus_tag="VFI55_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074240.1"
                     /translation="MPTIWTYLRATAVVAGSSAALIVGGLVGTAHADPAPVPAPMPNI
                     PQQLISSVANAPQILQNLATALGATPPAAPPSAPGISFPGVTPAAAAAAPSAPAAIPS
                     IPGLTQQAAPAAPTVPGVNGPLSLLAPQAPAAPAVPAAPAVPAAPAIPGLPISIPGLS
                     APAAPAVPAAPAVPAAPAIPAIPAMPAIPGITPPAAAPAPASVPQVPQAKIDMPALPG
                     LPVNVPAQVTLPGDLTALTSGTAPAAPAAAAPATPPSLLSALP"
     gene            9502..10642
                     /locus_tag="VFI55_09210"
                     /pseudo
     CDS             9502..10642
                     /locus_tag="VFI55_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215673.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1825 a         3758 c         3543 g         1629 t
ORIGIN      
        1 cagccgccgc cgataacgtt gctcatgggc cccacgtaga ggctcgttgc gaggtcgtac
       61 caggagatcg gcgcaacgta cgaggggatc tgcgcaccca ggagggtgtc gaaggtcacc
      121 tcaccggtgc ccagcaccga gttccagaag ttgaagacgt tgtcgatcac gccgttccaa
      181 gcgccggtgc caccaaagat ggctgtctcc aacgccgtct cttgggcaac cagcgagttg
      241 ttgaagccga actccccatc gaccaaggcg gtgttgaagc cgagctccat cgcctgcagc
      301 tgattaaaga atccggtctg cgacccggcc gtcaccaatg cctggaaggg cgacacccca
      361 taagagagca gagtgggaag cccggcgatc ccgacagcaa ggccgccgtt caatgcggcg
      421 ctgagctggg cacccagccc ggaaagcccg gtggcgaagt tcacaccgaa gcttggggtg
      481 acctcgaaga ggctcccgaa ggcggccagg ccggcgttca ggctgaccgg caggctaaca
      541 cccaggccgg gcagcgaccc ccccaagttg ccgaccaggg acgcgccggt ctgcaggaaa
      601 cccgaaagcc cggctcccag gccggaaagg ccaccactga agctgcccga aaacccgccg
      661 ctgagcacgg tgctcaaccc cgacaaccca ccagtcaggc tgcccccaaa ccctggcagg
      721 cctgcgctga agccgcccgc caggccggaa agcccggcac cgaagctgct cgacagcgca
      781 ccaccggcct gtccaagggc cgacagccca ccaccgaagt ccgccgccaa tgtcgcacca
      841 accggcgcga atcccgagaa gctgccaccc aggctgcccg acaacatggc atcgaacgaa
      901 gcgcccatct ccgaaacgcc ggcgctcagg ctggccccca gcccagacag cccgccgctc
      961 aagtccaccg ccagggcagg cgccagggtc tgcacgacgt tggcctcggc cgccgcatac
     1021 gcgctcgcac ccgacgtcat ggcctggacg aactgctcgt ggaacagtgc ggcctgcgca
     1081 ctgagctgct ggtagcccgc cgcatgctgg gaaaacagcg cggcgatctg tgccgacacc
     1141 tcgtcggccg cggcgggcag cacgcccgtg gtcgcggtgg cggccgcgaa gttggccgaa
     1201 ccgatggtcg aaccaatgtt tgctagatca gtcgctgcat ctgcgatcag ctgcggcgcc
     1261 gcgatgagaa aagacacgtt gtacctccca ggccagcacg aaccgcgtta cccccaaatg
     1321 ccttcaccct ggcccgaccg ggtgttagct acgagcgaag gataactcga tgtctcgttg
     1381 aaaacaggta tttgcacttg cagtcggtaa ttgatcatat tacataccct gcgaatgatt
     1441 caagcgctaa ttgcagcgtc ttagtccacc tgtagtcgcg attgcattgc cctcgcacgc
     1501 ggcgcgggcg gagtggctgg gactcggcta cgagccggct aggagccggt cttgatccat
     1561 tggccggcgt cgccgacaat gcccaccggt accgcacccg acaggctgac gttctgcacg
     1621 ctcgggccgg ccgcgacgat ggtggtgtcc tgcaagatcg gcaacaccgt cgacatgttc
     1681 cacaaccgcg gctcgaccgc ggtgatcacg tcgttgatgc tcttgctgcc gttgagggct
     1741 gcgtcgatgt tcgactggat gctgcggtcg cagattccgg tgaggttcga cggcgcctgc
     1801 accagcgcgc cgggctcggt cgggcggctg ggcggggcct gcgtcggcgt cagggaggtg
     1861 gtgcccggcg cggcgggcgg cgacgcggcc gcgggcgtag tggtgggcgt tacggcggtc
     1921 ggcacttgtg tggcctgcag cgcgggacag ccgtaccgcg aagccagcag cgtcgccaga
     1981 tttccgcccg cctgatgcca accgacgatc gcgtccacct ggttgttgtt caacgcgtcg
     2041 cgatacagcg ccaccgggtc gagcgcgagc acggtggcgg cgatgccgac gttgcgcaac
     2101 tggtcggcgg ccgtgttggc caccgcgacc gacgtcgggt cgttgaccgc caccccgatg
     2161 accagcgcca gctgttggcc gtccttgctg atccggccgc ggatgacttc cggtggaccg
     2221 gtattcgccg gtgtcggtgc cggtgacgcc gacgcgttgg gctcgacctg atatccggat
     2281 gccgccaaca acgccagcgc ggcctgggtg gtcatcgcgg ccggcgcggt cggctcgtag
     2341 cccggatcgc tcggggagcg gatctgggcc tggtccaacg tgacggtgtt gtcgctgccc
     2401 gcgcccaccg ccgccaacag gtcgacgtcg agcaacccca gaatcccctt gcggacctgg
     2461 gtgtcggaca gcttgggctc gttggcgcgc agggtgagtt gcatgacccg cggtgtcacg
     2521 atccgggcgg tccgcacgtc cgggatggcc gacaactggg cgaacgccgc cgagccgcca
     2581 tgcacctggg cgacctgggt gtcgccgttg cgcacggagt cggccagcgc ggccggcgcg
     2641 ccggcccggc ggaagaggat cagggcgggt ttggcgggcg cgccccaata gcggtcattc
     2701 cgcgcaatca ggatctcgtc gcgctggggg tcgatgcttt ccacccggaa ctgcccgccg
     2761 gtgaccggca gggcgcgggc caggccggcc gcgaacccgc cgggcacgtc cttgacgatg
     2821 tgcgccggca cgatgttgct gaacagctcc ttccacgccg gatacggttg cgagaaggtg
     2881 accaccgcct gcttaccgcc ctcgagcgac tgcacgccgg tgatcaggtc gtagcctgcg
     2941 gggtcgacca cccccggctg accgaccatc tgtcgccaca ggtaccagaa gtcgtcggcg
     3001 ccgatcgggg cgttgtcggt ccactgcgcc tcgggccgga tcttgtagct caccgtgaac
     3061 gggttctggt tggtcacctc cgcggatacc aacagggtcg ggtccatctc ccagcgcgaa
     3121 ccggtcggcg tgttgggatc gggcaccggc cggaacgcgc tcggcaacac cagcgcgctg
     3181 atcgccgcgt tcaccggcga cagatcggac agcagatgcg ggttgaaccc ggcgccgatc
     3241 gagtcgatgc ccacgatgat ctgagcgggc cgctgcggcg gtggcggtga gctgtgcgga
     3301 gtatcggtgc tttgcggcgc cggcggcggg ttgacggtgc acgccgccag catcactccg
     3361 accagcgaca ccagcgcgcc gaccgccagg aaaaggcggc gggttcgagt cggcacgctc
     3421 atcagggtat cgagcgcttt tcccggaatc tggctaacca cgcgcgcggc gcgcgtcacg
     3481 cggtcacgaa aacccgggcc ggatttcgca cctgcgttac gttcgcgcgc cggcggcgcc
     3541 aagtcgttaa ccccgagcct tcgcccgcgc ccggctacgc gcccgggtgg tcgcctccag
     3601 ctcgactttg cgcacccgca cgatctccgg agtcacctcg acacattcgt cggccgcgca
     3661 gaactccatg gcctgctcca ggtcgagctc gagcggacgg gccagcgtct cgatgacgtc
     3721 ggcggtcgat gaccgcatgt tggtcagctt cttctcccgg gtgatgttga cgtcgagatc
     3781 ctcggcacgc ggattgatgc cgacgaccat gccctcgtag gtgtcctgcc cgggctcgac
     3841 gaagaactga ccgcggtcgg ccagctggat catcgcgaac ggggtgatgg cgccggaccg
     3901 atcggacacc agcgagccgg tgtggcgggc ccggatctcc cccgcccacg gccgatagcc
     3961 gtcgaatacg gcgttggcga tgccggtgcc acgggtgagg gtgaggaagt cggtgcgaaa
     4021 gccgatcaac ccgcggctgg ggacgatgaa atccatgcga acccagcccg cggcgtggtt
     4081 ggccatctcc tccatgcggc ccttgcggcc ggccatcagc tgggtgacgg cgcccacgaa
     4141 ctcctcgggg cagtcgatag tcatcgcctc gaacggctcg tgcagctgac catcgatcgt
     4201 cctggtcacc acttgcggtt tgccgaccgt gagctcgaac ccctcccgac gcatcgtctc
     4261 gaccagcaca gcgagcgcaa gctcgccacg gccctgcacc tcccaggcgt cgggtcggcc
     4321 gaggtcgacg acgcggatcg agacattgcc gaccagttcg gcgtccagcc ggctgcgcac
     4381 catgcgcgcg gtcagcttgt ggccagacac cttgcccgcg agcggcgagg tgttggtgcc
     4441 gatggtgacc gagattgccg gctcgtcgac ggtgatacgt ggcagcgcat gggcatgatc
     4501 gggatcagcc agcgtgtcgc cgatcatgat ctcgggcagg ccggccacgg cgacaatgtc
     4561 gccggcgatc gcctcggcgg tggcgctgcg ttcaacgcct tcggtcgcca gcagttcggt
     4621 gatcttggcg ctggtgatga cgggcagccc atcgacctcg cgcatccacg cgacctgttg
     4681 gcctttgcgg atcttgccgt tgtagatgcg gatcagcgcc agccggccca gaaatgtcga
     4741 cgcgtccagg ttggtgacca gggcctgtag cggtgcctcg ggatcgcctt tcggcgccgg
     4801 cacgtatttt tccaggatct cgaacagcgg atccaggttg gcgccgtccg ggacatggcc
     4861 gtcgagaggt tgcgtggtgc tggccacgcc gacgcgcccg gaggcgtaca gcgtcggcag
     4921 acccagcgca tgctcagccg cggccgccgc ttcgtcgtcc agatccgacg ccacatcgag
     4981 caacagatcg tggctggcct ccaccacctc ggcgatgcgc gcgtcgggcc ggtctgtctt
     5041 gttgacgacg agaatcaccg gaagatgtgc ggccaacgcc ttgcgcagca cgaaccgagt
     5101 ctgaggcagt gggccctcgg acgcgtcgac cagcagcaac accccgtcca ccatcgacag
     5161 cccgcgctcc acctcaccgc cgaagtccgc gtgccccgga gtgtcgatca cattgatcac
     5221 tgtgacggtt ccatccgggt catggcggtg cacggcggtg ttcttggcca ggatcgtgat
     5281 gcctttttcc ttttccaggt cgccgctgtc catgatgcgt tcctgggtgt cgtcgccgcg
     5341 gtgggtcagg gcgccggact gccgcagcat cgcgtcgaca agggtggttt tgccgtggtc
     5401 gacgtgcgcg acgatggcga cattgcggaa tggcacgtcg gtgattctgg cagtgggggg
     5461 cgtggatggc gaactggcgg gctagacgac acccgtccat ccagtgtgcg cgcgggccgg
     5521 aacccccgga tgtcgccgcc ctggacgcac gccagaccgg gtacgagagc cttttcaaag
     5581 aactccggcg aggtcctcca gccagccgcg atcggcgggc acccagtcga tatcgtccaa
     5641 ttcggccgcg gtgacccagc gcagcgcccg gtggtcgtgg gggtgcggtt caccgctgat
     5701 cagccgcacc cgatacgccc gcagcgtcgc ggtggcattc agcgggatat ccacgccgag
     5761 gcggtcaccc acgacgatgt cggcgatctc gagtcccagt tcctcggcca actcgcgggc
     5821 cagcgcgtcg cgctcggttt caccgggcgc gaccttgccg ccgggcagtt cccagcggcc
     5881 ggccaactcg ggcggccgga cacgctgcgc gaccaggagc gctgagccgg cgatgacggc
     5941 cccggcgacg acgatctggg tcggcatggc cagtgacggt ataccgtcgg ggtgtcgcgg
     6001 tggtgtttca ccaccaacgt cgttggcgac cagcggaaac gccggcgctg atcattcggg
     6061 tcacgtaggc ccggctctcg gcgtagcttc ctcgcatgtc ccctttcaac acgatcctca
     6121 gaaacggcgc ggcgttcgag gcgcagctgc ggccgatatt ggaggagtac tgcgttgcgc
     6181 ttgatcttga agggtgcttt gacggactgt cggcgtccga agcccaccag gcagccgtgg
     6241 ccattctcgt cgccgtctgc ggccgggacg accctgctga ccatctacgc cagagtgcag
     6301 atgacatggt ggccaacgcg ttagccatca gccggtccct gcgcatcgct gcggcggtgt
     6361 ggtagcggcc ccgctgccgc accttcctcg acgagtcgtc gaggggtagc gttgccgctg
     6421 gtcgggaaac gaacacggga gaactgcaat ggcagccaag ttcgagatca gcaaagacaa
     6481 atcaggcaag tttcggttcc acctcaaggc ggccaacggt gagatcatca ccgccagcca
     6541 ggggtacgag accaaagcga gcgcccagaa gggcattgag tcggtcaaga cgaatgctcc
     6601 cggtgcggcg gtcgtacacc tcaccgaacg cgaacccgcc cacaactagc gcgtaacgcc
     6661 gttgctccct cgcacgccgc agcactgctt gcgcgtggcg caatcagcaa cgtgccccgc
     6721 gcccgcggtg gctcgtgaaa tgcattgacg caccgctgga tgcgcaccgc ggtgtgactg
     6781 taggcctacg gcagatgcag agattccttg tggcgcaatt cgtttcgacg tcgccacccc
     6841 ggtgtgagca gcccgtgtgc gccgatctga gagaatcgga cgggcatcga acgttcgggc
     6901 tgccgcgcag aacttcgcgg attcaaggcg tacatcccgc agtgggaact ggatttcaac
     6961 caagggagga taggtcgaag tatggctgtg ttaaccgatg aagaagtaga cgccgcactg
     7021 cccgaactca acggatggca gcgggccgac ggcgccctgc gccggtcgat caagttcccg
     7081 acgtttatcg ccggcatcga cgccgtgcgc cgggtcgccg agcacgccga gagcaaagac
     7141 catcatcccg acatcgacat ccgttggcgg accgtaactt tcactctcgt cacgcattcc
     7201 gcgggcggta tcaccaagaa cgacatcgcg atggcgcgcg acatcaacgg gatcgtcggg
     7261 agctaatcct ccgacgcggc ccgcacgcgg cgccgctgtg gcgccggcaa atcgggcccg
     7321 gtgtcgcgtc ggccggtgac cgcgatccag atcaacgtcg ccaccgccgc cacgacatag
     7381 accaacccgg cccaggccag ataccacggc cggcttattt gccagatggt cggctgcgcg
     7441 aaactcagca accacggcac gccgatgatg gtgagcgcca accagcccca gccgacgatc
     7501 ggcgcaccgg gtcggcagcg cggtggcccg tgaatcgccc agatcatcag cggcaccacc
     7561 cacacccagt ggtgcgtcca cgaaatcgga gagagcagca gcccgaacaa ctcgaccacc
     7621 agcaacttgc ccagctgatc gggcccgtcc agggcccgcc acgccaggac ggccagcacc
     7681 gccgtgacgg cgatggcggc cagcaccggc ggaccaaagc cggcgtcatg gccgaggatc
     7741 cgcgaaatgc cgccgcgcca cgtctggttg aacgaggtcg cgatcggccc gacccggtgc
     7801 gcgtcgccga gtagctccgt gaagtagtag cgagtctgat cgccgacgac caacacggac
     7861 accccgacgg tggccacgaa cacgagcgcc gagaacaccg ccgcggccca gcgccgagcg
     7921 cccacgaaat agacgccgga gatggccggc gtcaacttga tccccgccgc cacaccgacc
     7981 agcaggcccg aaagccacca ccgcgtggtg tagaccgccc acaacacccc cagcatgagc
     8041 aacacgttga tctgcccgta gtcgaacgtg ctgcgcagcg gctcaatcca gatggtgatc
     8101 gcggtccaca acattgccac gcgctgaccc gcgccggccg caacgccgat caaccgctgg
     8161 ctgatccgaa ccgcgccgta cagcgcggcc atcgtcgcga tctgccacag aaaggcgacc
     8221 aggccgaacg gcaagagatg cagcgggtag aagacgacgg ccgcgaaggg tggatacgtg
     8281 aacggcagcg ggaagtcggg tgtctggtcg gcgtacacgt agctgtacag ggtgccggga
     8341 tggtcgatag cggcggcgcc cccgaggtat acgtgcaggt cgacaaagtt cgcgccgttg
     8401 ggcatcaggt aggaccaggc gagtcgcgcc aggacgctca ggatcagcag cgccggggcg
     8461 gtcgcctgga gccgactcgc cagcctccgc gtgaacggtg ccgcctgggc ggccgaggtg
     8521 gtttctatct gcccgacttt agcgatcgca accgagacgg tggcgttgtc ggtcgtgacg
     8581 gccgtctgga gcacgcatgg gaatttgccg gcgagggacg ggttccgacc tcgcggtcgg
     8641 ccgttgggtc accgccgtca ctcgtctcgg taacgatcga ataaatgcca cacgtgtcac
     8701 ttgagtcaca tcagcatcaa agtaacttcg gcctcgggac ctgtcgtcaa caaccaggga
     8761 gagtcatgcc aaccatctgg acctacttgc gtgcaaccgc cgtcgtggcg ggttcgtccg
     8821 cggcgctgat agtgggcggt ctcgtgggaa cagcccacgc cgaccccgcg cccgttccgg
     8881 cgccaatgcc gaacattccg caacagctga tcagttcggt cgccaacgcg ccgcaaatcc
     8941 tgcaaaacct cgctaccgcg ctcggcgcga ccccgccggc cgcgccaccg tcggctcccg
     9001 gcatcagctt tccgggcgtg accccggccg cggcggcggc agcgccgtcc gccccggcgg
     9061 caatcccgtc gattccaggg ctgacgcagc aggccgcgcc cgcggccccg accgtccccg
     9121 gggtcaatgg gccgctctca ttgttggcac cgcaggcgcc ggcggcaccc gcggtaccgg
     9181 ccgctccggc cgttccggcg gctccggcga ttccgggact cccgatctcg atccccgggc
     9241 tgtccgcacc ggcggcaccg gcggtaccgg ccgctccggc cgtcccggcg gctcccgcga
     9301 ttccggcgat cccggcgatg ccggccatcc ccggcatcac cccgccggct gccgctcctg
     9361 cgccggcgtc tgttcctcag gtgccccagg cgaagatcga catgcctgcg ctgccaggtt
     9421 tgccggtgaa cgtccccgcg caggttacgc tgcccggcga tctcacggca ctgacctcgg
     9481 ggacggcccc ggcagcgccc gctgcagccg cgccggccac gccgccgtcg ctgctttccg
     9541 ctctgccgtg agcagcctgg tagacggcta acccacaaca ccgaggacgg agaaaatcgt
     9601 ggcaaacact tggaacctgt ccaagggttt ggccgctgtc gtcacggcgt cggctgccgc
     9661 gttcggggtc tgcccgagcg cggcggccga cccggcggca ccgcagccga caccgcagcc
     9721 gagctccaca cagcaactac cgggcctgcc ggcgctcgca cagctgagcc cgatcatcca
     9781 gcaagcggcc ggcaatcccg ggcaggcgac ggagctgctc atggctgccg cccaagcttt
     9841 cacccacaat ccgacggcgc cggccgagtc caaaaacgtt gccgcgtcgg tgaatcagtt
     9901 cgtgcaggag cccggcgcgc cgaacgccgc caccccgcct gacaccgcac cggcgctaca
     9961 cgtgccaccg gcaggcgtcg agccgggcct gcaatcgcac ctgcctccgg ggatcgatcc
    10021 ggctcgcgcg gcgggcccgg ggcccgccgc ttcgcccgcg gccgcgcctc cggtggcgac
    10081 gccgcccgtt tcggcaccgg caccggcggc cgtcgaccca gctaccgagc ccgtcgcggc
    10141 gcccgtaccg gcaccgccgg ctccggcgcc ggcaccggac tcccccgcgc cggccgccgc
    10201 ccctgcggca gcagctccgg gctttggtcc cgacgccccg gtcacccagg acttcatgta
    10261 tccctcgatc ggcaccaatt gccttgcgga cggcagtagc gccatcgcca ccgcgctgtc
    10321 cgtggcgggg ccggcgaaga tcccgtcgcc tggtcccgca ccgggtcaga cggcctacgt
    10381 gttcaccgcc gtcggcaccc ccgggcccgc cgacgtgcag aagctgccgt tgaacgtcac
    10441 ctgggtgaac ctcaccaccg gcaagtccgg aacggtcgcg ctcaagccgc gcgccgacat
    10501 caacgccgac ggaccgacca cgttgaccgc gatcgtcgac accggttcgg gcagcatcat
    10561 gtcgacgatc ttcgggcagg tcaccaccaa ggaaaagcag tgccagttca tgcccacgat
    10621 cggctcaacg gtggtgccct aaacagcccc cagcaagccc cagccgtgag cgtgcgtggg
    10681 cgtacgcgtc caacggcgtg tcgcggtacc gacacgcacg ctcgcggtca gcgacctaat
    10741 acgccatgaa caaga
//
LOCUS       DASSGQ010000211         4462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_318804,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000211 DASSGQ010000000
VERSION     DASSGQ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318804"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1494
                     /gene="lysA"
                     /locus_tag="VFI55_09215"
     CDS             145..1494
                     /gene="lysA"
                     /locus_tag="VFI55_09215"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET7074241.1"
                     /translation="MTLLDILPSIGKAAPRRLDPAIWPVTAHSDEEGRLCVGGVALAD
                     IADEFHTPTYVIDESDFRQRARRYRRLARRIHNMEVVYAGKSLLTTAVARWARDEGLG
                     VDVCSGGELAVALAGGVDPARIIMHGNAKSPHELHEAVRAGVGRIVLDSFIEIAYLAG
                     LARKRQPVLIRVTPDIDIHGHRAITTGISDQKFGFTLAEDQAADAVKRVLAHPILDLV
                     GLHCHIGSQVTDPALYGEAIHRMIAAMADIRARHGVILTELNIGGGHGIPYLPGDKGL
                     DLDELGAVIEDALDEACAAEHFPRPTIVVEPGRAISARAGVTLYRVCSVKTQPGGRTF
                     VAVDGGMSDNPRVSLYGAQYAVTLANRHSLGVKQRVTVAGRHCEAGDEIARDIELPAD
                     LHPGDLLAVACTGAYHHSMASNYNMVGRPPLVSVKDGRARLLVRRETISDLLARDCG"
     gene            complement(1524..2117)
                     /locus_tag="VFI55_09220"
     CDS             complement(1524..2117)
                     /locus_tag="VFI55_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7074242.1"
                     /translation="MTGSERRQQLVGIARSLFAERGYEGTSIEEIALRANVSKPVVYE
                     HFGGKEGLYAVVVDREMSALLDGITSSLTNNRSRVRVERVALALLTYVEERTDGFRIM
                     IRDSPASISSGTYSSLLNDAVSQVSSILAGDFARRGLDPGLAPLYAQALVGSVSMTAQ
                     WWLDTREPKKEVVAAHLVNLMWNGLTHLEADPRLQDG"
     gene            2310..2381
                     /locus_tag="VFI55_09225"
     tRNA            2310..2381
                     /locus_tag="VFI55_09225"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2343..2345,aa:Gln,seq:ttg)
     gene            2479..3939
                     /gene="glmU"
                     /locus_tag="VFI55_09230"
     CDS             2479..3939
                     /gene="glmU"
                     /locus_tag="VFI55_09230"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET7074243.1"
                     /translation="MSVCGDTAVLVLAAGPGTRMRSDTPKVLHTLGGRSMLSHSLHAI
                     TKVAPQHLVVVLGHDHQRIAPLVAELAAALGRTIDVALQDRPRGTGDAVRCGLSALPD
                     DYTGIVVVTAGDTPLLDADTLADLIAAHAAVSAAVTVLTTTLSDPHGYGRILRTQDNE
                     VMAIVEHADATPSQRDIREVNTGVYAFDVAALRSALSRLSANNAQQELYLTDVLAIVR
                     GDGRTVQARHVDDSALVAGVNNRVQLAELGAELNRRVVAAHQMSGVTIVDPATTWIDV
                     DVTIGRDTVIHPGTQLHGRTRVGSRCMVGPDTTLADVSVGDDASVVRTHGSSSSIGAG
                     ATVGPFAYLRPGTVLGADGKLGAFVETKNSVIGAGTKVPHLTYVGDADIGEHSNIGAS
                     SVFVNYDGETKRRTTIGSHVRTGSDTMFVAPVTIGDGAYTGAGTVIRDDVPPGALAVS
                     AGPQRNIEGWVQRKRPGSAAAQAASQAADAASGQTP"
     gene            4049..>4462
                     /gene="prsA"
                     /locus_tag="VFI55_09235"
     CDS             4049..>4462
                     /gene="prsA"
                     /locus_tag="VFI55_09235"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET7074244.1"
                     /translation="MSYDWTDNRKTLMLFSGRAHPELAEQVAKELDVHVTAQTAREFA
                     NGEIFVRFHESVRGCDAFVLQSCPDPVNNWLMEALIMIDALKRGSAKRITAVMPFYPY
                     ARQDKKHRGREPISARLVADLLKTAGANRILTVDLH"
BASE COUNT          744 a         1585 c         1428 g          705 t
ORIGIN      
        1 ccccggtgcg gtcatgatga ggccattcta ggggcctctt ctgagggcct cagggggcaa
       61 tgacttcgtc aatattgagc tcggcgccgc taagggatcg taaggattgc ggcacacgat
      121 cggttgcgcg cgcaccgtga agtcatgaca ttgctggaca tcctgccgtc tattggcaag
      181 gcggctcccc ggcggttgga tcccgcgatc tggcccgtca ctgcgcactc cgacgaggag
      241 ggccggctct gtgtcggcgg cgtagcactg gctgatatcg ccgacgagtt ccacaccccg
      301 acctacgtca tcgacgaatc cgacttccgt caacgcgccc gccgctaccg ccgcctcgcg
      361 cgccgcatcc acaacatgga ggtcgtctac gccggaaagt cgctgctgac caccgcggtg
      421 gcgcgctggg cgcgcgacga aggcctcggc gtcgacgtct gctcgggcgg tgagctggcc
      481 gttgccctgg ccgggggcgt cgacccggca cgaatcatca tgcacggcaa cgcgaaatcg
      541 ccccacgagc tgcacgaggc ggtgcgcgcc ggggtcgggc ggatcgtgct ggattcgttc
      601 atcgagatcg cctacctggc tggactggcc cgcaagcgcc aaccggtgct catccgggtg
      661 accccggaca tcgacatcca cggacaccgc gcgatcacca ccggcatcag cgaccagaag
      721 ttcgggttca ccctcgccga ggatcaggcc gccgacgcgg taaagcgcgt tctcgcgcat
      781 ccgatcctcg acctggtcgg gctgcactgc cacatcggct cccaggtcac cgaccccgcc
      841 ctctacggcg aggccatcca ccggatgatc gccgcgatgg ccgacatccg agcccgccac
      901 ggcgtcatcc tcaccgagct caacatcggc ggcggccacg ggatccccta tctccccgga
      961 gataaggggc tcgacctcga cgagctgggc gctgtcatcg aggacgcgtt ggacgaggcc
     1021 tgtgccgccg aacatttccc ccggccgacg atcgtggtcg aacccggtcg cgccatcagc
     1081 gcccgggccg gtgtgacgct gtatcgggtg tgttcggtga aaacgcaacc gggcggccgc
     1141 accttcgtcg cagtcgacgg cgggatgagc gacaacccgc gggtgtcgtt gtacggcgcg
     1201 caatacgccg tcacgctggc gaaccggcat tcgcttggcg tcaagcagcg cgtcaccgtg
     1261 gccggacggc actgcgaagc cggcgacgag atcgcccggg acatcgagct gccggcggac
     1321 ctgcaccccg gcgacctgtt ggcggtggcc tgcaccggcg cctatcacca cagcatggcg
     1381 tcgaactaca acatggtcgg ccggccgccg ctggtatcgg tcaaagatgg ccgggcccgc
     1441 ttgttggtgc gccgcgaaac gatctccgac ctgctcgcgc gcgactgcgg ttagtgccgg
     1501 gcgcaccgcg acgtctggtc cggctatccg tcctgtaggc ggggatccgc ttccaggtgg
     1561 gtcaggccgt tccacatcag gttgaccagg tgcgcggcga ccacttcttt tttcggctcc
     1621 cgcgtgtcga gccaccactg cgccgtcatc gacaccgagc ccaccagcgc ctgtgcgtac
     1681 agcggcgcca gacccgggtc caggccgcga cgggcgaaat cgccggccag gatggagctg
     1741 acctggctga cggcgtcgtt cagcaggctg gagtaggtgc cggagctgat cgaagccggc
     1801 gagtcgcgga tcatgatccg gaagccgtcg gtgcgctctt cgacgtaggt gagcagcgcc
     1861 agcgcgaccc gctcgacccg cacccgggac ctgttgttgg tcagcgacga ggtgatgccg
     1921 tcgagcagcg cggacatctc gcggtcgacg acgacggcgt acaaaccttc cttgccaccg
     1981 aaatgctcgt agacgaccgg cttggacacg ttcgcacgca gggcgatctc ttcgatcgag
     2041 gtgccctcat agccgcgttc ggcgaatagc gagcgcgcga tgccgacgag ttgctgtcgg
     2101 cgctcactac cggtcatgcg ggcgcgcggc acccgcactt ctttatctgc cttgtccggc
     2161 tcccggtcca gaacggccac atcaaccagc gtatcggttc gacggcaccg aaccatcgct
     2221 cccggctcgc ggccacaacc ggccgccaca ccgtctaaag tctttttggc gcggtcggtg
     2281 atcaaccgcc gatcgccgtt ggaacgcaat ccgtcgtggt gtaatcggca gcacatcaga
     2341 ttttggttct gagagttcag gttcgagtcc tggcgacgga gctgtgctct ttgccgcgag
     2401 cgtgcatgaa tgccacgctt gacggcgtgt cggcataaag acgcgcacgt tcgccagcgg
     2461 acactctagg agagttggat gtcggtttgt ggtgacaccg cggtcctggt tttagcggcc
     2521 gggccgggca cccggatgcg gtcggacacc ccgaaggtgc tgcacacgct cggcggccgc
     2581 agcatgttgt cgcattccct gcacgcgatc accaaagtgg cacctcagca tttggtcgtc
     2641 gtgctgggcc acgatcacca gcgcatcgcg ccgctggtcg ccgaactcgc cgccgcgctc
     2701 gggcgcacca tcgacgtcgc gctgcaggat cgcccacggg gcaccggcga tgcggtccgc
     2761 tgcggcctgt ccgcgctgcc cgatgactac accggcatcg tcgtcgtcac cgcgggcgat
     2821 acgccgctgt tggacgccga taccctggcc gatctgatcg cggcccacgc ggcggtgtcg
     2881 gccgcggtga cggtgctgac cacgaccctg agcgatccgc acggctacgg ccgcatcctg
     2941 cgcacacagg acaatgaagt catggcgatc gtcgaacacg cggacgcgac gccctcgcaa
     3001 cgcgatatcc gcgaggtcaa caccggtgtc tacgccttcg acgtcgcggc attgcgctcg
     3061 gcactgagtc gcctgagcgc caacaacgcc cagcaggagc tctacctcac cgacgtcctc
     3121 gccatcgttc gcggcgacgg ccggacggta caagctcgcc acgtcgatga cagcgcgctg
     3181 gtggccggcg tcaacaaccg cgtccagctg gccgaactgg gcgccgagct gaaccgccgc
     3241 gtcgtcgccg cccaccagat gtccggcgtc acgatcgtcg atccggccac cacctggatc
     3301 gacgtcgacg tcacgatcgg tcgcgacacc gtcatccacc ccggaacgca gctgcacggc
     3361 cgcacccggg tcggcagtcg ctgcatggtc ggcccggaca ccacgctggc cgacgtctcc
     3421 gtcggagacg acgcgtcggt ggtgcgcacc catggctcgt cgtcgtcgat cggtgcgggc
     3481 gccaccgtcg gccccttcgc gtacctgcgg cccggcaccg tgctgggcgc cgacggcaag
     3541 ctcggcgcat tcgtcgagac caagaactcc gtcatcggcg ccggcaccaa ggttccgcac
     3601 ctgacctacg tcggcgacgc cgacatcggc gagcacagca acatcggtgc gtccagcgtg
     3661 ttcgtcaact acgacggcga aaccaaacgg cgcaccacca tcggctcgca cgtgcgcacc
     3721 gggtcggaca ccatgttcgt ggctccggtg actatcggcg acggggccta caccggcgcc
     3781 ggcacggtca tccgcgacga cgtcccgcca ggggcgctgg cggtctcggc gggaccccag
     3841 cgcaacatcg aaggctgggt ccagcgcaag cggcccggca gtgccgccgc gcaggccgcc
     3901 tcgcaggccg ccgacgcagc ctccgggcag acgccgtgaa cgcgcgaccc ggccacccgg
     3961 caacacatcc aggcccttcg ctccgtacga ttggaccttc ccaccctatt gatcccaatc
     4021 tcgaccccga tagtgcgagg gcagtgcgtt gagctacgac tggaccgaca atcgcaaaac
     4081 cctgatgctc ttctccggcc gcgcacaccc cgaactggcc gagcaggtag ccaaagaact
     4141 cgacgtgcac gtcaccgcgc agaccgcgcg ggagttcgcc aacggcgaga tcttcgtgcg
     4201 cttccacgag tcggtgcgcg gctgcgacgc gttcgtcctg cagtcctgcc ccgacccggt
     4261 gaacaactgg ctgatggaag cactgatcat gatcgatgcg ctcaagcggg gcagcgccaa
     4321 gcggatcacc gccgtcatgc cgttctatcc ctacgcccgc caggacaaga agcaccgcgg
     4381 ccgcgaaccg atctcggcgc gactggtcgc cgacctgctc aagaccgcgg gcgctaaccg
     4441 aatcctcacc gtcgatctgc ac
//
LOCUS       DASSGQ010000212         7164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_258382,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000212 DASSGQ010000000
VERSION     DASSGQ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7164
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258382"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1145)
                     /locus_tag="VFI55_09240"
     CDS             complement(132..1145)
                     /locus_tag="VFI55_09240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET7074245.1"
                     /translation="MRVDVMTVPQPLGQVADLAQRTQAAGFSGLLLTETGRTAYLNAA
                     VASQAAPGLELSTGVAVAFPRSPFVTAATAWELQQATGGKFRLGLGTQVRTHVVRRYG
                     MAFERPGPRLRDYVLAVRACFSAFRTGTLDHHGDFYSLDFITPQWSPGPIDAPDPKVD
                     IAAVNPWMLRMAGEVADGVHVHPLGEPGYLKRHVLPNVAEGAAKAQRSPSDVAVIVPV
                     MTIVGDSDEERDNDRELVRASMAFYGSTPNYAFIWDEAGFEGTTARIREKQKTGDFEG
                     MAAQITDEHVATFATESTWDRLADTLAEKYGRIATRIVLYNALGDDERFRRYGEVARR
                     LQG"
     gene            complement(1153..2538)
                     /locus_tag="VFI55_09245"
     CDS             complement(1153..2538)
                     /locus_tag="VFI55_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7074246.1"
                     /translation="MSDMTARFEEIVGNTNLLAGDSIPEDYWHDEVLTTAPQKPAYVA
                     KPATAEEVSQLLKAASEHGVPVTARGSGSGLSGAAIPRKDGLLISFERMNAVVEVDTT
                     NQVAVVQPGVTLNELDAATAGSGLRYMVQPGELSSSVGGNVGTNAGGMRAVKYGIARH
                     NVLGLQAVLPTGEIIRTGGKIAKVSTGYDLTQLIVGSEGTLALATEVIVKLHPRLDHS
                     ATVLAPFADFDQVMEAVPKVLGSGLAPYILEYIDNMTMAALVHTQSLELGIPDQVRDS
                     CQAYLVVGLENRTADRLYEDVERTGELLADLGAVDAYVLEGGSARKLIEARENAFWTA
                     KAIGADDIIDTVVPRGSMPKFLAAARGLAAAADGAAAGCGHAGDGNVHLAIFCKDAAK
                     KKRLMTDIFSLAMELGGAISGEHGLGRAKTSYFLHLEDPVKVELMRRIKHGFDPAGIL
                     NPGVLFAADEA"
     gene            2636..3940
                     /locus_tag="VFI55_09250"
     CDS             2636..3940
                     /locus_tag="VFI55_09250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET7074247.1"
                     /translation="MLSLVHPVVGVPRPRLDSRRPLVAAMVALHGIELIALLCAAGFA
                     TRLARAHPGHGARLPTLLILAALVLVGWQACRLLRGRRSLNPSGLAVQSGVLMTSIAV
                     TTQNPTAGLIGIVLACAVLGGWGRLAESRRAAATFETASAAEDVVAAQTSRIGLGGVL
                     LVVLTALVILAALSVRSQIVFGLAVLAVIFVPLERLFALHPRRVLRRGWRTDVVHYLV
                     NGAALRIGLLVSVVVFGSVLRVFVPAPLRDAVAASPGWVQLTAGLAIAMIGAYAGHRA
                     AHEVPLLWRFHRVHHSIRQMDWLAANHLHPLDETFIRSVAVLPLYALGFGRVSLGAFV
                     IVITLQAIFIHANVRVNFGPLRWVIGTPQFHHWHHAREPRAYNSNYAGELPFLDALFG
                     TLYFPADRWPAQYGVDDTEPDGYLRQLAWPFRTRCEANAASH"
     gene            3962..4330
                     /locus_tag="VFI55_09255"
     CDS             3962..4330
                     /locus_tag="VFI55_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HET7074248.1"
                     /translation="MIEVLPDMPEGVTGIRVSGRLFGDDLRAFRPAMEGLLKTDEIRI
                     VEVIAPDYEGFGRGGLIEDLKLGFGALFQHHSAFKRIAVVSDKEWVAHTLHALAWMVP
                     GELALFGLDDLDRAKEWAAG"
     gene            4444..5859
                     /locus_tag="VFI55_09260"
     CDS             4444..5859
                     /locus_tag="VFI55_09260"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HET7074249.1"
                     /translation="MSVAMVAPDAISEQSETIQIAFSGRFGFGIDRWAYLPFEVPVGV
                     QRIRVTTSHDGFAVGELIRNVLDLGIFGAAGHDLGNVAGFRGWSGGARDGFTLSGAEA
                     TPGYLAGPIEPGLWAVALGPVVLNPLGMAWQARVTLERGPQPTALGAALPYSPPPARG
                     VGWYRGDLHLHTVHSDGQREPGELVTAAHAGGLDFIVSTDHNTNSANRAWPTCRTGGL
                     LVIPGEEVTTRHGHWLAVGLPPHAWVDWRYAPRDGVFPRFAADVRGAGGLVVAAHPAA
                     PLPGSLWEFGFDHVDALEVWNGRWNLDDEVSLRVWHRLLCEGRRVAAVAGSDSHTERQ
                     PVGSPQTVVHARELSMPAVIDGLRSGRSYIARSRSVTVELTASCRGAQVAGAGQTLRV
                     PPETPVTVTAMVTGVPGTNATLVSATGCVGRAMVDGSARTMLRWEIDAATARFARLEI
                     RDAQRRRLGAMVALTNPIWLG"
     gene            5903..>7164
                     /locus_tag="VFI55_09265"
     CDS             5903..>7164
                     /locus_tag="VFI55_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7074250.1"
                     /translation="MAAIPQTVYARDGDAHLAYQVVGDSGPDLLLVPTATFPIDLLWD
                     EPTVARHLRRLSSFSRLILTDLLGMGSSDAVPIQDRPAMQSWTDGLVAVLDAVGSECA
                     SVFGMSESALPAMLLAATHPQRVRSLVLFSPYARYLRADDYPCGMPEPTFTRYVDVFG
                     DTVGTGAVADNLAPSWAGDAGKRRWWARGERLAGGPGYFKAIFDLYLRTDVRPALDSI
                     QAPTLLIYRRGDRHVTRGHARYLAERIPHARLVELDGDDNAWFAGDSDRVLDEIESFV
                     TGARAAAPTNRVLSTVLFTDIVGSTERAETLGDEAWTAALDVHNEVVRKHVASARGEV
                     IKFTGDGALATFDGPARAITCACAIRDAVEDLGLDIRAGLHTGEVEMIDGDVHGIAVH
                     IAARIMALAGPGEVLVSGVVPPLVLGSR"
BASE COUNT         1032 a         2339 c         2470 g         1323 t
ORIGIN      
        1 gagaggggac acacttcgtg cccacctccg agcgacacgt tgccgcggtg attcagctgt
       61 cactatgagg cgggcacatc ggtatacccg gaactccttg ttacgcgtgg ggcttcgagc
      121 ccggcaatcc atcacccctg cagccggcgg gcgacctcgc cgtagcggcg gaagcgttcg
      181 tcatcaccca gtgcgttgta caagacaatg cgggtggcga tccggccgta cttttcggcc
      241 aacgtgtcgg ccaggcggtc ccacgtcgac tcggtggcaa acgtggcgac gtgttcgtcg
      301 gtgatttggg ccgccatgcc ctcgaaatcg ccggtcttct gcttctcgcg gatccgggcg
      361 gtagtgccct cgaagccggc ctcgtcccag atgaacgcat agttgggggt gcttccgtaa
      421 aaggccatgc tggctcgcac cagttctcga tcgttgtcgc gctcttcgtc gctgtcgccg
      481 acgatcgtca tcaccggcac gatcactgcg acgtccgacg gtgaacgctg cgctttcgct
      541 gcgccttcgg cgacgttcgg cagcacgtga cgtttcagat agccgggctc accgagcgga
      601 tggacatgca ccccgtcggc cacctcacct gccatgcgta gcatccacgg attcaccgcc
      661 gcgatatcca ccttggggtc aggcgcgtcg atgggtcccg ggctccactg cggcgtgatg
      721 aagtcgaggc tgtagaagtc gccgtggtga tcaagcgtgc ccgtccggaa tgccgagaag
      781 cacgccctca ccgccagcac gtagtcgcgc agccgcgggc cggggcgttc gaacgccata
      841 ccgtagcggc gcaccacgtg ggtgcgaacc tgcgtaccca aacccagacg gaatttcccg
      901 cccgtcgcct gctggagctc ccacgccgtg gccgccgtga cgaagggact gcgcggaaaa
      961 gccaccgcga cgccggtgga caactcgagc ccgggcgcgg cctgcgatgc cacggccgcg
     1021 ttcagatagg cggtgcggcc ggtctcggtg agcagcaggc cggagaaccc cgccgcctga
     1081 gtacgctggg ccaggtccgc gacctgcccc agcggttgcg gaaccgtcat cacgtcgacg
     1141 cgcacggcgc cctcacgcct cgtcggcggc gaacagcacg ccggggttga ggatgcccgc
     1201 ggggtcgaag ccgtgtttga ttcggcgcat gagttcgacc ttgaccgggt cttcgaggtg
     1261 caggaagtac gaggtcttgg cacgtccgag gccgtgttcg ccggagattg caccgcccaa
     1321 ttccattgcg agcgagaaga tgtcggtcat cagccgcttc tttttcgcgg catccttgca
     1381 gaagatggcc agatgtacgt tgccgtcgcc ggcgtgcccg cagccggccg cggcaccgtc
     1441 ggcggcggcc gccaaacccc gagctgcggc aaggaacttc ggcatcgacc cgcgtggcac
     1501 cacggtgtcg atgatgtcgt cggcgccgat cgcctttgcc gtccaaaacg cgttctcgcg
     1561 tgcctcgatg agcttgcggg ccgaacctcc ctcgagcaca taggcgtcga cggcgcccag
     1621 gtcagcgagc aactcacccg tgcgctcgac gtcttcgtac agccggtcgg ccgttcgatt
     1681 ctcaagcccc acaacaagat acgcctggca actgtcgcgg acctggtcgg gaatgcccag
     1741 ctccaggctc tgggtgtgga ccagggccgc catcgtcatg ttgtcgatgt attcgaggat
     1801 gtagggcgcg agtccgctgc ccaggacctt gggcaccgcc tccatcacct ggtcgaagtc
     1861 ggcgaagggc gctagcacgg tggcactgtg atccagccga ggatgcagct tgacgatcac
     1921 ctcggtggcc agggccaggg tgccttcgga gccgacgatc agctgggtca ggtcgtagcc
     1981 ggtggagacc ttggcgatct tgcccccggt gcggatgatc tcgccggtcg gcagcaccgc
     2041 ctgcaggccg agcacgttgt ggcgcgcgat cccgtatttg acggcgcgca ttccgccggc
     2101 gttggttccg acgtttccgc cgacgctgga ggacagctcg cccggttgca ccatgtagcg
     2161 cagcccggaa ccggcggtgg cggcgtccag ctcgttgagc gtcaccccgg gttggacgac
     2221 ggcgacctgg ttggtcgtgt cgacctccac gacggcgttc atccgctcga aggagatcag
     2281 caacccgtcc ttccgcggaa tcgcggcgcc cgacagcccg ctcccggacc cgcgtgccgt
     2341 caccggcacg ccgtgttctg aggcggcctt tagcagctgc gaaacctctt ccgcggtcgc
     2401 cggcttggcg acgtaggccg gcttttgcgg cgccgtcgtc aatacctcgt cgtgccaata
     2461 gtcttcgggg atcgagtcgc ccgccagcag gttggtgttc ccgacgatct cttcgaaccg
     2521 cgccgtcatg tcgctcatcg agggcctcac cttctgaccg tgcctgccgc ccagcgattc
     2581 actttatccc tgccggaagg cgacgaatct cagtcggttc tcaggtgcgc agagcatgct
     2641 gagcctcgtg catccggtgg ttggagtacc ccggccgcgt ctggactcgc gtcggcccct
     2701 ggtggcggcg atggtggcgc tgcacgggat cgagctgatc gcgctgttgt gcgccgcggg
     2761 tttcgccacg cggctggcgc gtgcgcatcc gggccacggc gcgcgcctcc cgacgctgtt
     2821 gatacttgcg gcgctggtgc tggtcggatg gcaggcctgc cgactgctcc gcgggcggcg
     2881 gtcactgaac ccatccggcc tcgccgttca atcgggggtc ttgatgacgt cgatcgcggt
     2941 gaccacccag aacccgaccg ctgggttgat cgggatcgtg ctggcgtgcg cggtgctggg
     3001 tggatggggc cgtctggccg aaagtcggcg cgccgctgcg acattcgaga cggcctcggc
     3061 cgccgaagat gtggtggcgg cgcagacgtc gcggatcggg ctcggcggcg tgctgctggt
     3121 ggttctgacg gcgctggtga tactggccgc gctgagtgtg cgcagccaaa tcgttttcgg
     3181 gttagcggtt ttggccgtca tcttcgtccc gctcgagcgg ctgttcgcgt tgcatccgcg
     3241 tcgggtgttg cggcgcggat ggcgcaccga cgtggtgcac tacctggtca acggcgctgc
     3301 gctgaggatc gggctgctcg tctcggtggt cgtgttcggt agtgtgctaa gggttttcgt
     3361 gccggcgccg ctgcgcgacg cggtggccgc gagcccgggc tgggtgcagc tcacggcggg
     3421 attggcgatc gcgatgatcg gcgcctatgc ggggcatcgc gcggctcatg aggtgccact
     3481 gctgtggcgg ttccaccggg tgcatcacag catccggcaa atggactggc tggccgccaa
     3541 tcacctgcac ccgcttgacg aaaccttcat tcggtccgtc gcggtgctac cgctttacgc
     3601 gctgggcttc ggccgggtca gcctgggtgc gttcgtcatt gtcatcacgc tgcaagccat
     3661 cttcatccac gccaacgtgc gggtgaactt tggcccgctg cggtgggtga tcggcacccc
     3721 gcagttccac cactggcatc atgcgcgcga gccgcgggcc tacaacagca actacgccgg
     3781 cgaattgccg ttcctggacg cgcttttcgg gacgctgtat ttccccgccg accgctggcc
     3841 tgcacagtac ggcgtcgacg acaccgaacc ggacggctat ttgcgtcaac tcgcatggcc
     3901 gtttcgcacg cgttgtgaag ccaacgcggc aagccattag cgctgaaata gggaggattc
     3961 gatgatcgag gttctgccag acatgccaga gggagtgacc ggcatccgcg tttctggacg
     4021 gctgtttggt gacgacctgc gcgcgttcag gccggctatg gagggcctgc tgaaaaccga
     4081 cgagatcagg atcgtggagg tcatcgcgcc cgattacgag ggcttcgggc gcggcgggtt
     4141 gatcgaggac ctcaagctgg gctttggtgc tctctttcag catcattcgg ccttcaagcg
     4201 catcgcggtg gtgtcggaca aagaatgggt tgcccatacg ctgcatgccc tggcctggat
     4261 ggtccccggc gagctcgccc ttttcgggct cgatgacctc gatcgcgcga aggagtgggc
     4321 cgccggctag cccccacagc gcacgaaccc gtcagtggtt cgacgaaagt tgttcgtgtc
     4381 gttgtaagag tggctacttt ttcgtgccgg acgctgggcg gtgtacccgt cgagaggacc
     4441 gctgtgagcg tcgcgatggt cgcccccgat gccatctccg agcagagcga gacgatccag
     4501 atcgcgtttt ccgggcggtt cgggtttggc attgaccgat gggcttacct gccgttcgag
     4561 gtgccggtgg gcgtgcagcg gatccgggtc accacctcgc atgacggctt cgcggtcggt
     4621 gagcttatcc gaaacgtgtt ggatctgggc atctttggtg cggccggcca cgacttgggc
     4681 aatgtggccg ggttcagggg gtggtccggc ggagcccgcg acggctttac cctctctggt
     4741 gccgaggcca ccccgggcta tcttgcgggc cctatcgagc ccggcttatg ggcggtggcg
     4801 cttggtccgg tggtcctgaa cccactggga atggcttggc aggcgcgcgt caccctggag
     4861 cgcgggccgc agccgaccgc tctaggcgcc gccctcccgt attcgccccc gcccgcccgc
     4921 ggtgtcggct ggtaccgcgg ggacctgcat ctacacaccg ttcactccga cggccagcgc
     4981 gagcccggtg aactggtcac ggccgcgcac gccggcgggc tggacttcat cgtctccacc
     5041 gaccacaaca ccaactcggc gaaccgcgcg tggcccacct gccgcaccgg cgggctcttg
     5101 gtaatccccg gcgaggaggt caccaccagg cacggccatt ggctcgccgt cggtttgcca
     5161 ccgcacgcct gggtcgactg gcggtacgca ccgcgcgacg gggtttttcc gcgcttcgcg
     5221 gccgacgttc gcggggccgg cggcttggtg gtcgccgccc atcccgccgc gccgctgccg
     5281 ggatcgttgt gggagttcgg ttttgaccac gtggatgcgc tcgaggtgtg gaacgggcgg
     5341 tggaacctcg acgacgaagt gtcgcttcgc gtttggcatc ggctgctgtg cgagggtcgg
     5401 cgcgtcgcgg cggtcgccgg cagcgactca cacaccgaac gccagccggt gggttcgccg
     5461 cagacggtcg tgcatgcgcg tgaactgtcg atgcccgcgg taatcgacgg gttgcgcagt
     5521 ggccgttcgt atatcgcccg atcacgttct gtcacagtgg agctcaccgc gtcctgccgg
     5581 ggcgcccagg tggctggggc cgggcaaacg ctgcgggtgc cgcccgagac gccggtcacg
     5641 gtcaccgcga tggtcaccgg ggttccgggc accaacgcga ctctcgtttc cgcaactggc
     5701 tgtgtggggc gggcgatggt ggacggctcg gcgcggacca tgttgcgatg ggagatcgat
     5761 gccgcaactg cgcgcttcgc gcggctggag atacgggacg cgcagcggcg ccgactgggt
     5821 gcgatggtcg cgttgacgaa cccgatctgg ctggggtgac gcgcggcctc accgggtggg
     5881 gaccgagtgg catgctttgt gcatggcggc catcccgcag accgtatacg cgcgagacgg
     5941 cgatgctcac ctcgcctatc aggtcgtcgg tgacagtgga ccggatctgc tgctcgtccc
     6001 gactgccacg ttcccaatcg acttgctttg ggacgagccc actgtcgccc gccacctgcg
     6061 tcggctgtca tcgttcagtc gcctgatcct caccgacctg ctcggaatgg gaagctccga
     6121 tgcggtgcca atccaggacc gtccggcgat gcaatcttgg acggatgggc tggtcgcggt
     6181 gctcgacgcc gttggcagcg agtgcgcgtc agtcttcggg atgtcggagt cggcgttgcc
     6241 tgccatgctg ctggccgcca cccatcctca gcgcgtgcgc tcgctggtgc tcttcagccc
     6301 gtatgcgcgc tatctgcggg ccgacgacta tccgtgcggg atgcccgagc cgacctttac
     6361 ccggtatgtg gacgtgttcg gcgacaccgt cgggaccggg gccgtcgcgg acaacttggc
     6421 cccgagttgg gccggggatg ccggtaagcg gcgctggtgg gcgcgcggcg agcggctggc
     6481 cggcggcccg ggatacttca aggcgatctt cgacctttac ctgcgcaccg acgtccggcc
     6541 cgcgctcgac agcatccagg cgcctacgct gctgatatac cgtcgcggtg accgccatgt
     6601 gacccgcggt cacgctcgtt atctcgccga acgcattccg cacgcgcgac tggtggaact
     6661 cgacggggac gacaacgcgt ggtttgccgg cgactccgac cgcgtcctcg acgagatcga
     6721 atcgttcgtc accggtgcgc gtgccgccgc gccgacgaat cgggtgctgt cgacggtgtt
     6781 gttcaccgac atcgtcggat cgaccgaacg cgccgagacg ctcggcgatg aggcgtggac
     6841 ggccgccctc gacgtgcaca acgaggtcgt gaggaagcat gtggccagcg cgcggggcga
     6901 agtcatcaag ttcaccggcg acggcgcgct tgccaccttc gatgggccgg cgcgcgcgat
     6961 cacctgcgcg tgcgcgatcc gagacgcggt ggaagacctc gggctcgaca tccgtgccgg
     7021 cctgcacacc ggcgaggtgg agatgatcga cggcgacgtg cacgggatcg cggtccatat
     7081 cgccgcgcgc atcatggcgc tggcgggccc cggagaggta ctcgtgtcgg gcgtggtccc
     7141 gccgctggtc ctcggatcga ggat
//
LOCUS       DASSGQ010000213         3250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_227268,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000213 DASSGQ010000000
VERSION     DASSGQ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227268"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..409
                     /locus_tag="VFI55_09270"
     CDS             20..409
                     /locus_tag="VFI55_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074251.1"
                     /translation="MMQEPAQTSPILALVAAARVGVGAAMLLAPGRFIKPESGPETLL
                     LQTIGIRDLVLGSGACAALLRGGGGEFRRWATVGLISDSADLLLGLRSRPLVGTRSAL
                     IATLAPVPFVGAAVLGFTRNLRTLRSR"
     gene            complement(833..1180)
                     /locus_tag="VFI55_09275"
     CDS             complement(833..1180)
                     /locus_tag="VFI55_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074252.1"
                     /translation="MTLTVEQLAHIREVADRWVNAGLSTQRCDRPRAEASVRSAYHTA
                     GLDEPQLIVWMDSPVGGMFAPAVIKSAATTMPLANHVWSLIGEQPWDRMVDQLGIQQA
                     QHLWTQISDQLAD"
     gene            complement(1177..1620)
                     /locus_tag="VFI55_09280"
     CDS             complement(1177..1620)
                     /locus_tag="VFI55_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074253.1"
                     /translation="MFSSRLISTLAAVGTACLGLAACAAAHPSAANASTHPDSPTCVG
                     LSICTPPPPDAEGNPPCYYADGWQADASEGGIAVWYFHEPANLSKADQVTAVVRMKDG
                     TVASQIAQIDAGQQLHRFGFPGIDKSAVQEVLLSSGGGQCFVTGP"
     gene            complement(1674..2846)
                     /locus_tag="VFI55_09285"
     CDS             complement(1674..2846)
                     /locus_tag="VFI55_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7074254.1"
                     /translation="MKTVTCTNTKFEVVDWPTPTPAKGQLLIDVMKCGICGSDLHARH
                     HCDELADVMAESGYHAFMRSNQQVVFGHEFCGEVVDYGPGTRKTPRRGTPVVSVPLLR
                     RGKEVHGIGLSTMAPGAYAEQLLVEQSLTFAVPNGLAPEVAALTEPMAVGWHAVRRGE
                     VGKGDVAIVIGCGPIGLAVICMLKAHGVRTVIASDLSPGRRALATACGADIVVDPAQD
                     SPYTTEAGRAHLQGMLEAFDLAVGTIEKLQRLRLPWWHVWRVAEKAGAATPKHPVVFE
                     CVGVPGIIDGIITGAPLFSRVVVVGVCMGSDHIRPAMAINKEIDLRFVLAYTPLEFRD
                     TLHMLADGKVNVAPLITGTVGLSGVEAAFDALGDPEAHAKILIDPKSTAATPQPVG"
     gene            complement(2877..>3250)
                     /locus_tag="VFI55_09290"
     CDS             complement(2877..>3250)
                     /locus_tag="VFI55_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015575361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET7074255.1"
                     /translation="WLVVDVRDLAALHSALVEPGRGPRRYTAGGHRVPAAELASMLGQ
                     IAGTPMIAVPIPDTALRVAGAVMDRAGRFLPFDTPFTSAGMQYYTQMPASDDSPSERE
                     LGITYRDPRETVADTFEALRG"
BASE COUNT          544 a         1053 c         1077 g          576 t
ORIGIN      
        1 tgtgactagg cttggacccg tgatgcaaga accggcccag acatcaccga tcttggcgtt
       61 ggtggcggcg gctcgcgtcg gggtgggagc cgccatgctc cttgcgccgg gccggttcat
      121 caagcctgag tcggggcccg aaacgctgct cttgcagacg atcgggattc gcgacctggt
      181 cctggggtcg ggtgcgtgcg cggccctatt gcgtggtggc ggaggcgaat tccggcgctg
      241 ggcgaccgtt ggtttgatca gcgatagcgc cgaccttctg ctcgggttac gcagcaggcc
      301 cctcgtcggt accaggagcg cgctgatcgc cacgctggct cccgtgccat ttgtgggcgc
      361 ggcagtgctc ggctttacgc ggaatctgcg aacattgcgc agcaggtgag cggcgtcaac
      421 ggccgtcgta acacgtaaag gtgtagccgt cggcatacgc caatgccggc ccatcggcac
      481 catctcggcg gccagacgtg tcgccacaga tcaccgttgg acgatcggtc aggacaacgg
      541 tctcgttcat cggccaccac cagtccactt cacggcatgc cgcacaaacg gcgtcaagcc
      601 gaggtgagtt cgccaatccc gcaagggata gcgcacaggt catggaggcc agccaggcgg
      661 catcccgcca caggaaaagc ccctcccaca cttcatcacc gacgctgatc cggccgttgc
      721 cgagggggtc tcgaagatgg tcgttcagct ggtcgccaag ccggtcccca tacccattgt
      781 tgagctggcg ctccagcggg tccgcgagcc gatttttgag ctggtctccg agtcagtccg
      841 ccagttggtc ggaaatttga gtccaaaggt gttgcgcctg ttggattccc agttgatcta
      901 ccatccggtc ccaaggctgt tcgccgatta gcgaccatac gtgattcgcg agcggcatcg
      961 tcgtcgcggc gctcttgatg actgctgggg cgaacattcc acccaccgga gaatccatcc
     1021 acacgatgag ctgaggttcg tcgagtcccg cggtgtggta ggcggaccgc acgctggctt
     1081 cggcgcgggg ccggtcgcag cgttgggtgg acaaaccggc attcacccac cgatcggcga
     1141 cctcgcggat gtgggcgagc tgctcgacgg tcagtgtcac ggacccgtca caaaacattg
     1201 accgcctccg gaactcagca ggacctcctg cacggctgac ttgtcgattc ccggaaaccc
     1261 gaagcggtgc agctgttgcc ctgcatcgat ctgggcgatc tgggaagcga ccgtgccgtc
     1321 tttcatgcga accaccgcag tgacttggtc agcctttgac aggttcgcgg gctcgtggaa
     1381 ataccacacc gcgatcccgc cttcggatgc gtcagcctgc caaccgtcgg cgtagtagca
     1441 cggcggattg ccctcggcgt ccggcggcgg tggcgtgcat atcgagagcc cgacgcatgt
     1501 cggggagtct ggatgggtgg atgcgttggc cgcggatggg tgtgcagctg cgcaggccgc
     1561 aaggcccagg caggccgtcc cgacggcggc cagcgtgctg attagacggc tagagaacat
     1621 gcgcaaatta cagaccgcgc gcgttgctac cgatcccaat gtcagcgccg cggtcagccg
     1681 accggctgcg gggtggcggc ggtgctcttg gggtcgatca ggattttcgc gtgcgcctcg
     1741 gggtcgccta atgcgtcgaa cgcggcctcg acgccggaca gcccgaccgt cccggtgatg
     1801 agcggcgcga cgttgacctt gccgtcggcg agcatgtgca gcgtgtcgcg gaattcgagc
     1861 ggggtatagg ccagcacgaa ccgcaggtcg atctccttgt tgatcgccat cgccggccgg
     1921 atgtggtctg agcccatgca gacgccgacc acgacgacgc gcgagaacag tggcgcgccc
     1981 gtgatgatgc cgtcgataat ccctgggacg ccgacacatt cgaagacgac ggggtgctta
     2041 ggcgttgcgg cgccggcctt ttcggcgacg cgccaaacgt gccaccaggg cagccggagc
     2101 ctttgcagct tttcgatggt gccgaccgcc aggtcgaacg cctcgagcat gccttgcaag
     2161 tgtgcgcggc ccgcctccgt cgtgtagggc gagtcctgtg cgggatcaac gacgatatcg
     2221 gctccgcagg ccgtcgcgag cgcgcgccgg cccggtgaca agtcgctggc gatcacggtg
     2281 cgcaccccgt gcgccttgag catgcagatc accgcaagac cgatcggtcc gcagccgatc
     2341 acgattgcga cgtcaccttt gcccacctcg ccgcgcctaa cggcgtgcca cccgacggcc
     2401 atcggctcgg tcagcgcggc tacctcgggg gccaatccgt tcgggacggc aaacgtcagt
     2461 gactgctcga ccagaagctg ctcggcgtag gcgcccggcg ccatcgtcga caacccgatc
     2521 ccgtgcacct ctttgccccg ccgcaacagc ggcacggaaa cgaccggtgt gcctcgccgg
     2581 ggggttttgc gtgtaccggg gccgtaatcg accacctcgc cgcagaactc gtgcccgaac
     2641 acgacctgct ggtttgatcg catgaaggcg tggtagcccg attcggccat cacgtcggcg
     2701 agttcgtcgc agtggtggcg cgcatgcagg tccgatccgc agatgccaca cttcatcacg
     2761 tcgatcagca actgaccctt tgccggtgtc ggggtcggcc agtcgactac ctcgaacttc
     2821 gtgttggtgc aggtcacggt cttcatcgcg ccgtacctcc tgtcgttgca atgttgctac
     2881 ccgcgaagcg cctcgaatgt gtcggccacg gtttcccggg gatcccgata ggtgatgccg
     2941 agttcacgct cgctgggcga atcatccgat gccggcatct gcgtgtagta ctgcatcccg
     3001 gccgaggtga acggcgtgtc gaagggcaga aaccggccgg cccgatccat caccgccccc
     3061 gcaacgcgca gcgcggtgtc gggaatggga acggcgatca tgggcgtacc ggcaatttgg
     3121 ccgagcatgc tcgccagctc ggcggcggga acccgatgac cacccgcggt gtagcggcgt
     3181 ggcccgcgac cgggctccac gagcgccgag tgcagggcgg cgaggtcgcg gacgtccacg
     3241 accaaccatg
//
LOCUS       DASSGQ010000214        12819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1169701,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000214 DASSGQ010000000
VERSION     DASSGQ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12819
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169701"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VFI55_09295"
     CDS             complement(<1..276)
                     /locus_tag="VFI55_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7074256.1"
                     /translation="MASADRVFLALANPVRRELLEILTRQPLSAGELSDRFELSRPAV
                     AEHLKVLRDAGLVADEPQGRRRIYRLTAEPLAELGDWLHPFEKFWRAR"
     gene            366..1115
                     /locus_tag="VFI55_09300"
     CDS             366..1115
                     /locus_tag="VFI55_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7074257.1"
                     /translation="MHFAWEQLTAAVYRCRLPFCDVTIGLVRGHAGALLVDTGSTLVE
                     AAAIDADVRRIAGRPVSHVVLTHKHFDHVLGSSVFTGAAQAAGAAGAEVYCAPEVVEH
                     LSSATDQLRGDALAHGADAAQIDRAIAALRPPRHGIYDAVIDLGDRTVTIAHPGRGHT
                     TSDLVVVVAPGVGDDGRAVVFTGDLVEESGDPAIDADSDVVAWPETLDRLLALGGPRA
                     IYVPGHGNVVDAEFIRRQRDWLGGRADYQPS"
     gene            1285..3573
                     /gene="aceA"
                     /locus_tag="VFI55_09305"
     CDS             1285..3573
                     /gene="aceA"
                     /locus_tag="VFI55_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase ICL2"
                     /protein_id="HET7074258.1"
                     /translation="MAIIETETEVRTPFEQDVEATQRYFDGSRFARTTRLFTARQVVE
                     QRGTIPTDYPVAREAAAAFYDRLRELFAAKKSITTFGPYSPGQAVSMKRMGIEGIYLG
                     GWATSAKGSTTEDPGPDLASYPLSQVPDDAAVLVRALLTADRNQQYLRLHMSERQRAT
                     TPAVDYRPFIIADADTGHGGDPHVRNLVRRFVEIGVPGYHIEDQRPGTKKCGHQGGKV
                     LVPSDEQIKRLNAARFQLDVMRVPGIIVARTDAEAANLIDSNSDERDQPFLLGATNLE
                     IPSYKGCFLALMRRFYELGVKELNGHLLYALADGEYGIANAWLERQGILGLVSDAVNT
                     WRENGKDKIDDLFDQVESRFVAAWEDDAGLMTYRDAVAVVLSFGVREGEPATMSPEEW
                     RKFSARIPHYAAREKARELGADPPWSPELARTPEGYYQVRGGIPYAIAKSLAAAPFAD
                     ILWMETKTADLADAREFAEAIHAEFPEQMLAYNLSPSFNWDTTGMTDEEMKRFPEELG
                     KMGFVFNFITYGGHQIDGVAAEEFATSLQQDGMLALARLQRKMRLVESPYRTPQTLVG
                     GPRSDAALAASSGRTATTKAMGKGSTQHQHLVQTEVPKKLLEEWLALWSDHYQLGEKL
                     RVQLRPRRAGSDVLVLGIYGDEDEQLANVVFDPIKDRHGRSILTVRDQNTFAEKLRQK
                     RLMTLIHLWLVHRFKAKAVYYVTPTEDNLYQTSKMASHGIFSEVNQEVGEIIVAEVNQ
                     PRIDELLKPDRVALRKLITKEG"
     gene            3863..4093
                     /locus_tag="VFI55_09310"
     CDS             3863..4093
                     /locus_tag="VFI55_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074259.1"
                     /translation="MKALRRTAVAIFGGAAALALTVGFGGIALGPAGSASATPTHASS
                     SVTQARPNAAAPAADAGVHTATLTGCIAGLDC"
     gene            4514..5683
                     /locus_tag="VFI55_09315"
     CDS             4514..5683
                     /locus_tag="VFI55_09315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7074260.1"
                     /translation="MAKLRFGYFIAPFHRAGTNPTLALQRDLEFVEHLDALGFDEVWL
                     GEHHSAGSEIISSPEIFIAAAAERAKRIRFGTGVISLAYHNPLWVADRLMLLDHLTHG
                     RVLGGMGPGSLPTDSAMIGLTPTDTRELLETNLDIVVRLLAGETVSAKTATHQLFDAR
                     LQLAPYSDGGIPLAVAAVASPTGARLAGKHGIGLLSIGATLIVEGFDALAYHWGIAEE
                     RAAAFGTRVDRRNWSLVGPFHLAETDEQARADVKFGIEPWFRYFQRVAAFPQMTMPGE
                     QLDEMIDIINDNGAGVIGTPERARAQVQRLWDQSGGFGCMLQMGHEWANPAATRRSAE
                     LFAAEVMPHFQGQAQPTVDAAARAGQVRNDLAQTQLQAIEHMTKKYESEKRPTQD"
     gene            6023..7159
                     /locus_tag="VFI55_09320"
     CDS             6023..7159
                     /locus_tag="VFI55_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018180694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HET7074261.1"
                     /translation="MEIYADGLRGKLPRFPVDYASLERAAIEVLPSWVYSYVGYGAGD
                     GNTQRANIEAFSRYAIVPRMLVAPTERDLSVSLFDMQLPSPLFMPPVGVVGVCSQDLH
                     GDIQAAQASARTGVPMVASTLTQDRMEDVVPHAGETPNLFQLYTSNDKELAGSFVHRA
                     ERSGYKAIVVTLDTWITGWRPHDLNMANFPQLRGYCLQNYFTDEVFLARLSKSPQDDP
                     QAAALEWAKVFGHPVRWDDLGWLRSLTKLPMLLKGIQHPDDVRKARDHGIDGIYCSNH
                     GGRQANGGLPSLEALPAVVKAADGMPVLFDSGVRSGSDVAKALALGATAVGLGRTYLY
                     ALAIGGVDGLVSQLRAVLAELDLLMAVDGYPSIADLRAAGAQRI"
     gene            complement(7538..9097)
                     /locus_tag="VFI55_09325"
     CDS             complement(7538..9097)
                     /locus_tag="VFI55_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005087692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7074262.1"
                     /translation="MVDVVIAGAGPNGLMLAGELGLAGIRPVVLEPMPGPNPQRRANG
                     IVGQGVRILDHRGLYSTITGSAEPPRLNPRAMFAAFPLDLALVPDSQLFLLPVQQPRL
                     VQVLADRAAEYDVDIRWGHALTGFDQDADGVTAHVTGPDGVYQLRAKYLVGADGGTSM
                     TRKLAGIDFPGMSSYDIVARLGFDVLPPDEWIDPVSGGLDVPGFGRVPPLQFHRAEQG
                     VFAFGALGSRAATIVFELDTTAREEPPDDNVDGSPMSLAELEASAKRVLGAEVPLRSA
                     SPDGPLDLRRFSGINSRIASRYQVGRVILVGDAAHVHSPMGGPGLNLGLQDAVNLGWK
                     LAAVLDGRVDPALLATYEAERRPAAERVIMHSRAQLALVRPGPEVTALRQLFSELVTD
                     PDIVRRLGDLISGAENRYSTGSAHPLAGRWVPDFSVANGRGTTRVAELARDGRPLLVD
                     LTERAVVAATVDCDVGVAGQLTVAAGRPLGDVPATALLVRPDGYVAWASSAATPDPDE
                     LREVLARWFGI"
     gene            9223..9657
                     /locus_tag="VFI55_09330"
     CDS             9223..9657
                     /locus_tag="VFI55_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074263.1"
                     /translation="MTTTPHRPSRQTHAAGTRRRQLRVMQLIFAGAAAIAMFALFDAA
                     RPDTTETMLGSGSVRLADMAGDLPAPASGPQFPQTPPNLSGGNGFAADDSNDQAQLQE
                     QQSLQQMQQEMQQAQEQNDAAEQQFLDGMQQAQMDEQQANNP"
     gene            complement(9739..10206)
                     /locus_tag="VFI55_09335"
     CDS             complement(9739..10206)
                     /locus_tag="VFI55_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7074264.1"
                     /translation="MSGRTYSFEINRTSSASAATLFRLVADGASWSTWAKPIVLSSSW
                     ARQGDPAPGGVGAIRKVGAWPVFVQEETVEYEQDRRHAYKMVGPPSPAKDYSGEVTFT
                     PNAAGGTDIRWAGSFVEGVPGTGPVMRAALGGAVRFFADRLVKAAERESGGGR"
     gene            complement(10310..11716)
                     /locus_tag="VFI55_09340"
     CDS             complement(10310..11716)
                     /locus_tag="VFI55_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HET7074265.1"
                     /translation="MKTRQGRRGERRGFIQGLTALCVRYVERLMPDPYLFAVLLTLIV
                     VLLVAVLVRGATAAGVLKAWYGGVWGAQNIFTFAFQMVLVLVTGYTLAEAPILKRAII
                     NVASKPTNQVQGALLCFGVSTVFSLLNWGLGLVSGALVARQVAKRLSGAHFGYLIASG
                     FMGYIVWTQGLSSSIALANTDTTSPINVIHKLTGMTVPLTLTIFEPYNWIAVIVVAVL
                     LALAIWRMEPTQGLEPDPAVFEEEPAPKRPEGKRTFAEWLENLWILNVVVFAAGITYF
                     VLSGFALNISSMIMLFTVTSALLHGTPIRFIRAFSNAAKVSGPLLLQYPLYGGIVGLL
                     GYLPTGSGKALQTVLAEAVVRGADQYTLPFLTFIGSVLISLFVPSGGGHWAVQGPIAV
                     GSAQAIGQHSPAYLGLISMSVAVGEGVANMIQPFWLLPVLAIAKLNVRQVMGFTVIAF
                     LIGFAVFSAMVLIAPHVL"
     gene            12023..12793
                     /locus_tag="VFI55_09345"
     CDS             12023..12793
                     /locus_tag="VFI55_09345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019925087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7074266.1"
                     /translation="MNGVAHEAMDDLTGRTAMVVGASRGLGRGIAVAAAQAGAAVIAV
                     ARSATTFSEPAAGPGSIQAVIADAADASVPGSLLDRYDPDVLVLVAGASPLVRPLQYH
                     TWETFSANWNADVRIAFHWLREALLKPLRPGSRVVVISSGAALAGSPLSGGYAGAKAT
                     QRFITGYAQEEANRAGLAIAFTAVMPRITPLTELGRPAVRAYAARSGQSEEEYVRQLG
                     VPLSPEVAGAAVVELMRADTAAVAPGYLLTGTGLQKPA"
BASE COUNT         2328 a         4316 c         4131 g         2044 t
ORIGIN      
        1 tcgggcccgc cagaacttct cgaacggatg cagccagtca cccaactcgg ccagcggttc
       61 cgcggtcagg cggtagatgc gcctgcggcc ttgcggctcg tcggcgacca gccccgcgtc
      121 gcggagcacc ttgaggtgtt cggcgacggc cgggcgactg agctcgaacc ggtcgctgag
      181 ttctcccgcg gacaggggct gccgggtgag gatctcgagc agttcccgcc gaaccggatt
      241 cgccagggcg agaaagacgc gatcggcgct ggccaccccg gcaatatatg tcggaaatta
      301 ccgacatgtc aactatttcc gacatatgcc gacgatctgc gccggtgacg gctaccttcc
      361 tccttgtgca tttcgcgtgg gagcagctga cggccgccgt gtaccggtgc cggttgccgt
      421 tctgcgacgt cacgatcggg ctggtgcggg gccacgccgg ggcgctgctc gtcgacaccg
      481 gtagcacgct ggtcgaagcg gcggcgatcg acgccgacgt ccgccggatc gctggacgcc
      541 cggtcagcca tgtggtgctg acgcacaagc acttcgacca tgtgctgggt tcatcggtat
      601 tcacgggcgc cgcgcaggcc gcgggagccg cgggggccga agtgtactgc gcacccgagg
      661 tcgtcgagca cctgtcctcg gccaccgacc agcttcgcgg cgacgccctg gcccacggcg
      721 cggacgcggc gcagatcgac cgcgccatcg cagccttgcg cccgccgcgg cacggcatct
      781 acgacgccgt catcgacctt ggcgaccgga cggtaaccat cgcccacccg ggccgcggcc
      841 acaccacgtc ggatctggtc gtggtggtgg cgcccggggt cggcgacgac ggccgggccg
      901 tggtgttcac cggcgatctc gtcgaagagt ccggcgaccc ggcaatcgat gccgattccg
      961 acgtggtggc ctggccggaa acgctggatc ggctactcgc cctgggtggg ccccgcgcca
     1021 tctacgtccc gggtcacggg aacgtcgtcg acgcggaatt catccgccgg cagcgggact
     1081 ggctgggcgg acgagcggac taccaaccca gctaacaggc aggtcggcgg cggaccgggc
     1141 cgcccgagcg ggcctcaccc tgggtcggag cctcgaatag ggcatcgcac caccgttgcg
     1201 ccccgtcgac catcgacccg tgattgaacc gccaagtccg gggctaaact ctcgtcagca
     1261 cactgtgaag aggagcgaaa tcgtatggcc atcatcgaga ccgagaccga ggttcgcacg
     1321 ccgttcgagc aggacgtgga ggctacccag cgatacttcg acggctcgcg ctttgcccgg
     1381 accacccgcc tcttcactgc ccgacaggtc gtggagcagc gcggcacaat tccgaccgat
     1441 taccccgtgg cgcgcgaggc ggcggcggct ttctacgacc gcctgcggga gctcttcgcc
     1501 gcgaagaaga gcatcaccac gttcggcccg tactcgcccg ggcaggccgt gagcatgaag
     1561 cggatgggca tcgagggcat ctacctcggc ggctgggcga cctcagccaa gggctccacc
     1621 accgaagacc ccggcccgga cctcgccagc tacccgctca gccaggtgcc cgacgatgcc
     1681 gcggtgctgg tccgcgcgct tctcaccgcc gaccgaaacc agcagtacct gcgtctgcac
     1741 atgagcgaac ggcagcgcgc caccaccccg gccgtcgact accggccgtt catcatcgcc
     1801 gacgccgaca cgggccacgg cggtgacccg cacgtgcgca acctggtccg ccgcttcgtc
     1861 gagatcggcg tgccgggcta ccacatcgag gatcagcgcc cgggcaccaa gaagtgcggc
     1921 caccagggcg gcaaggtgct ggtgccgtcg gacgaacaga tcaagcgcct caacgccgcc
     1981 cgcttccagc tcgacgtcat gcgggtgccc ggcatcatcg tcgcgcgcac cgacgccgag
     2041 gcggccaacc tgatcgacag caactccgac gagcgtgacc agccattcct gctgggcgcg
     2101 accaacctgg agattccttc ctacaagggc tgtttcctgg ctctcatgcg gcggttctac
     2161 gagctcggcg tcaaggaact caacggtcac cttttatatg cccttgccga tggcgagtac
     2221 ggcatcgcca acgcgtggct cgagcggcag ggcattcttg gcctggtctc cgacgccgtc
     2281 aacacctggc gggagaacgg aaaggacaag atcgacgacc tgttcgacca ggtcgagtcg
     2341 cggttcgtgg cagcctggga ggacgacgcg ggactgatga cctaccggga tgccgtggcg
     2401 gtggtgctct cattcggtgt acgcgaaggc gagccggcga ccatgagccc cgaggagtgg
     2461 cgcaagttct ccgcgcggat accccactac gcagcccgcg agaaggcaag ggagctgggc
     2521 gccgacccgc cgtggagccc cgagctggca aggacccccg agggctatta ccaggtgcgc
     2581 ggcgggatcc cgtatgcgat cgccaagtcg ctcgcggcgg cgccgttcgc cgacatcctc
     2641 tggatggaga cgaagaccgc ggacctcgcc gacgcccggg agttcgccga ggcgatccat
     2701 gccgagttcc ccgagcagat gctggcctac aacctctcgc cgtcgttcaa ctgggacacc
     2761 accggcatga ccgacgagga gatgaagcgc ttccccgagg agctcggcaa gatgggcttc
     2821 gtcttcaact tcatcaccta cggcgggcac cagatcgacg gtgtcgccgc cgaggagttc
     2881 gcgacctcgc tacagcagga cggcatgctg gcgctggcgc gtttgcagcg caagatgcgc
     2941 ctggtcgagt cgccctaccg cacgccgcag accctcgtcg gcggaccacg cagcgacgcc
     3001 gcgctggccg catcgtcggg ccgcaccgcc accaccaagg cgatgggcaa gggctcgacc
     3061 cagcatcagc atctggtgca gaccgaggtg cccaagaagc tgctcgagga gtggctggcc
     3121 ttgtggagcg accactacca gctcggggag aagctccgcg tgcagctgcg gccccggcgc
     3181 gccggctcgg atgtgctggt gctcgggatc tacggcgacg aggacgagca acttgccaac
     3241 gtggtcttcg atcccatcaa ggatcgccac ggccgcagca tcctcaccgt gcgggatcag
     3301 aacaccttcg ccgagaagct ccgccagaag cggctgatga cgctgatcca cctctggctc
     3361 gtgcaccggt tcaaggccaa agcggtgtac tacgtcacgc cgaccgagga caacctgtat
     3421 cagacctcga agatggcatc ccacgggatc ttcagcgagg tcaaccagga ggtcggcgag
     3481 atcatcgtcg ccgaggtcaa ccagccgcgc atcgacgagc tactcaagcc cgaccgcgtg
     3541 gcgctgcgga agttgatcac caaggaaggg tagcggcggc cccgcacttc cctctcaccg
     3601 cgccggcgag cgccggcggc tcatgcgcgt cgaccgcggc cgcgcccgcg ggcctcgcgg
     3661 cggcgcgatt agcgatcctt tagcgccctt ttaccgaccc tttttaacga ccctttggcg
     3721 ggtcacggac ctccccgccg gtcgcgcacc ggcgcgtccg tcaaagcctt ccctgcagca
     3781 atagttgcat tgatgtatag ttagctgcat acaaacacag tcggatgtga caaacaccca
     3841 cggtaggaga ggagagactg caatgaaggc actccgtagg acggcggtgg caatcttcgg
     3901 gggtgcggcc gcgctcgcgc tgacggtggg atttggcggc attgccctcg gcccggcagg
     3961 cagcgcctcg gcaacgccga cgcacgcatc gtccagcgtg acgcaggcgc gccccaacgc
     4021 agccgccccc gcagcggacg ccggagtaca caccgcgacc ctcacgggct gcatcgccgg
     4081 cctcgactgc tagaacgaca agcgaaccag gccgttggat cctcaccgcc aacggcctgg
     4141 ttcgttttcg gggcccttga tgtcgcggtc taggccctct cgaaatttgc caccgaaccc
     4201 gctggcgcgg cgtgcggacc cgccgacaca gctccgcccg tcaggccccc gcacccgttt
     4261 gcgcatacgc atgccggggt acggataggt ggaccacagc gagtgctgct agtggaaagt
     4321 gggattctga tgcgatctcg agcgaggacc gcggtcgggg ttctcgtcgg tgcagctcgt
     4381 gttgacggac acacaaaggc ttgctcctca tagccgatgg ggccatgttg atcctggccc
     4441 catcggctga gcaatatctc gggtcgggca ccgtgcgtta tggttcgggc agtgcgactg
     4501 caaagggact tcaatggcga agctcaggtt tggatatttc atcgccccat tccaccgcgc
     4561 cggcaccaat ccgacgcttg ccttgcagcg tgacctggag ttcgtcgaac atctcgacgc
     4621 cctcggcttt gacgaggtgt ggttggggga gcaccactcc gcgggcagcg aaatcatcag
     4681 ctccccggag atcttcattg ccgctgccgc cgaacgtgcc aagcggattc ggtttggcac
     4741 cggggtcatt tcgctggcgt accacaaccc gctctgggtc gccgaccggt tgatgctgct
     4801 ggaccacctc acccatggtc gtgtactggg cgggatggga ccgggctcgc tgcctaccga
     4861 ctccgcgatg atcggactca cccccaccga tacgcgcgag cttctcgaaa ccaacctcga
     4921 catcgtggtc cggctgctgg ccggggagac ggtcagcgcc aaaacggcca ctcaccaatt
     4981 gttcgacgcc cgtttgcagc ttgcgccgta ctccgacggc gggatcccgc tggcggtcgc
     5041 cgcggtcgcg tcaccgaccg gcgcgaggct ggccggcaag cacggcatcg gcctgctctc
     5101 catcggggcg accctgatcg ttgaaggctt cgatgcgctc gcctaccact ggggcatcgc
     5161 cgaggagcgc gcggcggcct tcgggacgcg ggtcgatcgc aggaactgga gcctggtcgg
     5221 ccccttccat ctcgccgaga ccgacgagca ggcccgggcc gacgtgaaat tcggtatcga
     5281 gccgtggttc cggtacttcc aacgggtcgc cgcgttccct cagatgacca tgcccggaga
     5341 gcagctcgac gagatgatcg acatcatcaa cgacaacgga gcgggtgtca tcgggacgcc
     5401 ggagcgcgcg cgggcccagg tgcagcggct gtgggatcag tcgggtggct tcggctgcat
     5461 gctgcagatg ggccacgagt gggccaaccc ggcggcaacc agacgatccg ccgaattgtt
     5521 tgcggccgaa gtgatgccgc attttcaggg gcaggcgcaa ccgactgtgg atgccgccgc
     5581 gcgcgccggc caagtgcgaa acgaccttgc gcagacgcag ctgcaggcca tcgagcacat
     5641 gaccaagaaa tatgaaagcg aaaaacgtcc tacacaggat taaagcccgt gggcagcagg
     5701 tgaagcgtgc tcgccgcaag cacccgaggt ctcggcctgc ccacaaccgg aacttgaagt
     5761 cgtgcgacag cggtcaaacc cgttgccgga cagagttgga gcaccgatgt gtcaccctcg
     5821 tgtcgacatt gatctggatg cagcgccagt gcggtcattg tgtcgaacga caacaggatg
     5881 cgtgtgcacg ttgtcatgct gaataccttc gatcaccgtt gccggtggga ggctcgtgaa
     5941 ggcggatttg atcatgccga accaccggcg gcggcgagta cgggagggta gcgtgccaac
     6001 acccaattac ggcgattacc aaatggaaat ctatgcagat ggcctgcgag ggaaactgcc
     6061 tagatttccg gtcgattatg cctcgctgga gcgtgcggcg atcgaggtgc tgccctcctg
     6121 ggtgtactcc tacgtcgggt atggagcagg cgatggaaat acccagcgcg ccaacatcga
     6181 agccttctcg cgatatgcga tcgtgcctcg catgctggtg gctccgaccg aacgagacct
     6241 gtcggtgtca ctgttcgaca tgcagctgcc cagcccgctg tttatgcccc ccgtcggagt
     6301 tgtcggcgtg tgctctcagg acttgcatgg cgacatccag gcggctcaag cctccgcgcg
     6361 gacgggagtc ccgatggtgg cgtcaaccct gacgcaggat cggatggagg atgtcgtccc
     6421 gcacgctggt gagaccccga atctgtttca gctctacact tccaacgaca aggagcttgc
     6481 tggtagcttc gtgcaccgcg ccgaacggtc cggctataag gcgatcgtgg ttaccctgga
     6541 cacctggatc accgggtggc gaccgcacga tctgaacatg gctaattttc ctcaactgcg
     6601 tggttattgc ctgcagaact acttcaccga tgaagtcttc ttggcgcggc tgtccaaatc
     6661 gccgcaggat gatcctcagg cggcggccct cgaatgggcg aaggtatttg gccatccagt
     6721 acggtgggat gacttgggct ggttgcggtc attgaccaag ttgcccatgc tgctcaaggg
     6781 tattcaacac cccgacgatg tgcgcaaagc gcgcgatcac ggtatcgacg gcatctattg
     6841 ctccaaccac ggcggccgcc aggccaacgg tggtcttccc tcactggaag ctctgccggc
     6901 cgtcgtcaag gcggcggacg gcatgccggt gctcttcgat tcaggcgtgc gcagtggttc
     6961 cgacgtcgcc aaggctttgg cgttaggcgc taccgccgtt ggtctgggcc gcacctacct
     7021 ctatgccctg gctatcggcg gtgtcgacgg tctcgtgtct caactgcgtg ccgtgcttgc
     7081 cgaactggac ctactcatgg ccgtcgacgg ctaccccagc attgccgacc tccgcgccgc
     7141 aggtgcgcaa cgcatctgac gaattactcg acgatccaat gcccgatcgc tgccgtcgaa
     7201 cttcgaaaat cgtcctgctg caaagtgttt caagacccag cagtccggca tgagcgaccg
     7261 cacactcgcg ctggccccgg agccactacc attacttcga gaacgccagc gccaccgaga
     7321 aattggtggc cctcatcgcg gtagttcggt tgatcgtcgc gaaaagcact acagcacact
     7381 gtagttcaag caacgaacac tacaccgccc cgagcgtcgc agcaccctag gagcgctagg
     7441 ggccggaatt agggcgtgag cgtcaaattc tcggtgaccc gtggttgtcg ggtggcgaac
     7501 agccctcgtt tacgggacga ggtcggttcg aagccgctca gatcccgaac cagcgcgcga
     7561 gcacttcccg taattcgtcg gggtcgggcg tggccgccga cgatgcccat gccacatagc
     7621 cgtccgggcg cacgagcagg gcggtggccg gcacgtcgcc gagcgggcgc ccggcggcga
     7681 cggtgagctg gccggcgacg ccaacgtcgc aatccacagt tgccgccacc acagcgcgct
     7741 cggtgaggtc gacgagtagc ggccgtccat cgcgggcgag ctccgccacg cgcgtcgtcc
     7801 cacggccgtt ggccacactg aagtcgggca cccaccgccc ggccaatgga tgcgcactgc
     7861 ctgtgctgta acggttttcg gcccccgaaa tcaggtcgcc gaggcgacgc acgatgtcgg
     7921 ggtcggtgac gagctcggaa aagagctggc gcagggctgt cacctcgggc ccgggccgga
     7981 cgagagccag ctgcgccctg ctgtgcatga tcacccgctc ggcggcgggc cggcgctcgg
     8041 cttcgtaggt ggccagcaga gccggatcca cgcgtccgtc gagcacggcg gcaagcttcc
     8101 agcccaggtt caccgcgtcc tgtagcccca ggttgagccc tggtccaccc atgggggagt
     8161 gcacgtgcgc ggcatcgccg accaggatca cccggcccac ctggtaacgg gatgcgatcc
     8221 gcgagttgat gccggagaac cggcgcagat caagcggccc gtcgggcgat gcggaccgca
     8281 gcggcacctc ggcgcccagc acccgcttgg cgctggcctc caactccgcc aggctcatcg
     8341 gcgagccgtc gacgttgtcg tcggggggtt cctcgcgcgc cgttgtgtcc agctcgaaga
     8401 cgatcgtggc cgcccggctg ccgagcgcgc cgaatgcgaa gacgccctgc tcggcgcggt
     8461 ggaattgcag gggcggtact cgaccaaacc ccggtacgtc gagccctccg ctgaccgggt
     8521 cgatccattc gtccggcggc agcacatcga agcccagccg ggcgacgatg tcatacgacg
     8581 acatgccggg aaaatcgatg ccggccagct tgcgggtcat gctggtgccg ccgtcggcgc
     8641 cgacgaggta cttcgccctg agctgataaa cgccatctgg tccggtgaca tgtgccgtga
     8701 cgccgtcggc atcctggtcg aaaccggtca gcgcgtgacc ccaacgaatg tcgacgtcgt
     8761 actcggcggc gcgatcggcg agcacctgca ccagcctggg ttgctgaacg ggcagcagga
     8821 acagctgcga atccggcacc agcgcgagat cgagcgggaa ggcggcgaac atcgcgcggg
     8881 ggttgagtcg cggcggctcc gccgagccgg tgatggtgct gtagagaccg cgatggtcga
     8941 ggatccggac gccctgaccg acaatgccgt tggctcgccg ttgcggattg ggccccggca
     9001 tcggttccag caccacgggc cggatcccgg ccaatcccag ttcgccggcc agcatcagcc
     9061 cattcgggcc agcgcccgcg atcaccacat caaccactta gccaagctta cgagtttggg
     9121 gtggctccga caaccgtctg gccgcgtgac agcgggtgat ctcacattcg ggcgatagcc
     9181 gcttctcagc ccacagctaa tgacgaggcg caaacatgat gcatgaccac cacccctcac
     9241 cgcccgtcgc gccaaaccca cgcagccggc acccggcgtc ggcaattacg cgtgatgcaa
     9301 ctgattttcg cgggggccgc ggccatcgcg atgttcgcgc tgttcgacgc cgcccggccc
     9361 gacaccaccg aaaccatgct cggatcgggt tcagtccggc tggcggacat ggccggtgac
     9421 ctgccggcac ccgccagtgg ccctcagttc ccccagacac cgcccaacct ttccggcggg
     9481 aacggcttcg ccgccgacga cagcaacgac caagcgcaac tacaagagca gcagagcttg
     9541 cagcagatgc aacaggagat gcagcaggcc caggaacaaa acgacgcggc cgagcagcag
     9601 ttcctcgacg gcatgcagca ggctcaaatg gacgagcagc aagccaacaa cccatagtcc
     9661 tgcgtcgagg cgtggcggcc gcgtggtgcg gccgtcatgc gctatctggc gaaacccctt
     9721 aagccacagg ggtaaacgct atcggccgcc gccggactcg cgctcggccg ccttgaccag
     9781 tcgatccgca aagaaccgga ccgccccgcc cagcgcggcc cgcatcaccg ggcccgtgcc
     9841 gggtacaccc tcgacgaaag agccggccca gcggatgtcc gtgccgcccg ccgcgttcgg
     9901 cgtaaaggtc acctcgccgg agtagtcctt ggcggggctc ggaggtccga ccatcttgta
     9961 ggcgtgacgc cgatcctgct cgtattcgac cgtttcctcc tggacgaaca cgggccacgc
    10021 cccaaccttg cgaatggcac cgacgccgcc cggcgccgga tcaccctgtc gcgcccaact
    10081 cgacgacagg acgatcggct tggcccacgt cgaccagctg gcgccgtcgg ccaccagccg
    10141 aaagagtgtc gcggcagacg cgctgctggt ccggttgatc tcgaacgaat acgtacgacc
    10201 cgacatgacc gactccctgg ttaagaccgc accgacccaa aagggaactt gccgcgtacc
    10261 ctacccgcgc aattagtccg cccaaccggc cgggctcggg tggacgatgt cagagcacgt
    10321 ggggcgcgat gaggaccatc gcgctgaaca ccgcgaaacc gatgaggaag gcgatcaccg
    10381 taaagcccat cacctgacgg acgttcagct tcgcgatggc gagcaccggc agcagccaga
    10441 acggctggat catgttcgcg actccctcgc cgaccgcgac cgacatcgag atcagcccga
    10501 ggtaggccgg cgagtgctga ccgatggcct gcgccgagcc gaccgcgatc ggaccctgca
    10561 ccgcccagtg tccgccgccc gaaggcacga acaacgagat gaggaccgac ccgatgaatg
    10621 tcaggaacgg cagcgtgtac tgatcagcgc cgcgcaccac cgcctcggcg agcaccgtct
    10681 gcagcgcctt gcccgatcct gtgggcaggt aacccagcag accaacgatg ccgccgtaca
    10741 gggggtattg cagtagcaac ggaccagaca ccttggccgc gttcgaaaat gcgcggatga
    10801 agcgaatcgg cgtcccgtgc aacaacgcgc tcgtgacggt gaacagcatg atcatcgacg
    10861 agatgttcag cgcaaagccg ctcagcacga agtacgtgat gcccgccgcg aagacgacga
    10921 cgttgaggat ccacaagttc tccagccact cggcaaacgt ccgcttgcct tcggggcgct
    10981 tgggtgccgg ctcctcttcg aagaccgcgg gatcgggttc gagaccctgc gtgggctcca
    11041 tccgccagat cgcgagggcg agcagcacgg ccaccacgat gacggcgatc cagttgtagg
    11101 gctcgaagat cgtcaacgtc aacggcacgg tcatgccggt cagcttgtga atgacgttga
    11161 tagggctggt ggtatcggtg tttgccagcg cgatcgagga cgacaggccc tgtgtccaga
    11221 cgatgtagcc catgaatccg ctggcgatga ggtagccgaa gtgggcgccc gacaggcgct
    11281 ttgccacctg gcgggcgacc agcgcacccg acaccagccc gagtccccag ttcagcaggg
    11341 agaaaacggt gctgacacca aagcacagca gggctccctg cacctggttg gtcggcttac
    11401 tcgcgacatt gatgatggcc cgcttcagga tgggcgcctc ggccagcgtg taacccgtca
    11461 ccaggacgag gaccatctgg aacgcgaacg tgaagatgtt ctgcgctccc cacactccgc
    11521 cgtaccacgc cttcagaacg ccggcggccg tcgcgccacg gacgagcacc gcgacgagca
    11581 agaccacgat gagggtgagg aggaccgcga acaaataggg gtcgggcatg aggcgctcca
    11641 cgtaacgcac gcagagcgcg gtgaggccct ggataaagcc gcgtcgctcg ccccgtcgtc
    11701 cctgtctcgt tttcatcgtt tgcctcgttt tcatcgtttt ccgtttctgg gctgtctcgt
    11761 cgcccgcatc gttatctggt tgcctaatcg aaccccagcc gcaggcaact gcaaacacgt
    11821 ttcgagcggg tgaatttcga ggactccgcg agcccttcgt cgacggcgct cggcagatcg
    11881 aacggcgccc cgcaggttcg gaaaaaagcg aggcccgccg tcgcgatgat tttcggcgcc
    11941 gcgagttgtc tatatatcga gaagtcctcg aagcccccgc accacagtgc gggggcttca
    12001 tctttggaaa aggggtaacg ccatgaacgg ggtcgcacat gaagcgatgg acgacctaac
    12061 cggccgcacc gcaatggtcg tgggcgcaag ccggggcctg ggccgcggca tcgcggtggc
    12121 tgcggctcag gccggcgccg cggtgatcgc cgtggcccgc agcgcaacaa cgttttccga
    12181 gccggcagcc ggccctggaa gcatccaagc ggtgatcgcc gatgcggccg acgcatcggt
    12241 gcccggcagc ctcttggacc gatacgatcc ggacgttctc gtgctggtgg ccggagcaag
    12301 cccgctcgtg cgtccgctgc agtaccacac ctgggaaacg ttctcggcga actggaacgc
    12361 cgacgtgcgt atcgcattcc actggctgcg agaggccctg ctcaagccct tgcgacccgg
    12421 cagcagggtg gtggtcatca gcagcggcgc cgccttggcc ggatcaccgc tcagtggcgg
    12481 ctacgccggc gccaaagcca cccaacgctt tatcaccggt tacgcacaag aggaggcgaa
    12541 ccgcgccggt ttggctatcg cgttcaccgc ggtgatgccc cggatcaccc cgttgaccga
    12601 actcggccgc cccgccgtgc gcgcgtacgc ggcccgcagt ggccagtccg aggaggagta
    12661 cgtcaggcag ctcggcgtac cgctcagtcc ggaggtggcc ggcgccgccg tcgtggagct
    12721 gatgcgggcc gacaccgccg ccgtcgcccc cggctatctg ctgaccggca ccgggctaca
    12781 gaagcccgcc tgatcggcgc cccttttacc aaaagtcaa
//
LOCUS       DASSGQ010000215         3638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_227335,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000215 DASSGQ010000000
VERSION     DASSGQ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227335"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1710)
                     /locus_tag="VFI55_09350"
     CDS             complement(61..1710)
                     /locus_tag="VFI55_09350"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HET7074267.1"
                     /translation="MSKAAELMVKCLENEGVSVVFGIPGEENIRFIQALAHSKSVRYV
                     LTRHEQAAAFMAEMYGRVTGRAAVVSATLGPGAINMQLGVADATTNSTPMVAISAQVG
                     QDREYKESHQYVDLVSMFAPITRWAAGVPTAHAIPEMFRKAFKLAETERPAAVYLAVP
                     EHIDADATDYDLKPLPRNVVRAEAPAPRQVARAVDILRNAQRPVVLAGHGAARGDATK
                     ALLRFSDALGIPVANTFHGKGVMPDDHPNSIGTLGFMRHDYVNFGFDNADAVIAVGYE
                     LQEFDPVRINPQADKRIIHIHRFPAEVDMHYPVDVGIIGDISASLDALTDALAGHAFE
                     PQTEVPGCGLLAEEFVRGQQDSRYPLAPARVVADTREALGRSDAVLVDTGATKMWMAR
                     LYPTYERNTCLISNGLSTMGFALPGALGVKLARPAAKVLAVVGDGAFLMNSQEIETAV
                     RERIPLVVLIWEDGGYGLIEWKMDLELGAHYYVKFGNPDIVTYSESFGAKGYRINSAA
                     ELLPTLKEALDDDGVSLICCPVDYSENLRLTDRLGELDETL"
     gene            1800..3176
                     /locus_tag="VFI55_09355"
     CDS             1800..3176
                     /locus_tag="VFI55_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinic semialdehyde
                     dehydrogenase"
                     /protein_id="HET7074268.1"
                     /translation="MPIATINPATGETVKTFTPATDDEVDAAIARAHERFLDYRHSTT
                     FAQRAEWANATADLLEKEADETAAMMTLEMGKTLASAKAEALKCAKGFRYYAENAEKL
                     LADEPADASKVGAKKAYIRYQPLGVVLAVMPWNFPLWQAVRFAAPALMAGNVGLLKHA
                     SNVPQSALYLSDVITRGGFPEGCFQTLLVSSGAVERILRDPRVVAATLTGSEPAGQSV
                     AAICGDEIKPTVLELGGSDPFIVMPSADLDEAVKTAVTGRVQNNGQSCIAAKRFIVHT
                     DIYDAFVDKFVERMEGLKVGDPTDPDTDVGPLATESGRDEIAQQVDDAIAAGAKIRCG
                     GTRIDRPGWFYPPTVVTDITKDMALYTEEVFGPVASMYRASGVDEAIEIANATTFGLG
                     SNAWTNDEAEQERFIDGIEAGQVFINGMTVSYPELGFGGVKRSGYGRELAGLGIREFC
                     NAKTVWVG"
     gene            complement(3194..>3638)
                     /locus_tag="VFI55_09360"
     CDS             complement(3194..>3638)
                     /locus_tag="VFI55_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="R2-like ligand-binding oxidase"
                     /protein_id="HET7074269.1"
                     /translation="EGMLALTGYYLWHKICVERGILPGMQELVRRIGDDERRHMAWGT
                     FTCRRHVAADDANWAVFEARMNELMPLGLRLIEEGFTLYDPMPFNLSVDEFMQYAADK
                     GMRRFGTISSARGRPLGEIDLDYSPLQLEDTFADEDEKALAGVGA"
BASE COUNT          636 a         1202 c         1183 g          617 t
ORIGIN      
        1 gccctcgccg ccgagaccca agtgaccgat gtgattacgc cgcaaagcgg atcccggtgg
       61 ctagagcgtt tcgtcaagct cgccgagccg gtcagtcaac cgcagattct cggagtagtc
      121 gaccgggcaa cagatcagcg aaaccccatc atcgtcgagg gcttccttca gcgtgggcaa
      181 cagctccgcg gcgctgttga tccgatatcc cttggcgccg aagctttccg agtaggtcac
      241 gatgtcggga ttgccgaact tcacgtagta atgtgctccg agttcgaggt ccatcttcca
      301 ttcgatgagg ccgtaaccac cgtcctccca gatcaacacc accagaggta tccgctcccg
      361 aacggccgtc tcgatctcct gtgagttcat caggaaggcg ccgtcaccga ccaccgcgag
      421 caccttcgcc gcgggccgtg ccagcttgac cccgagggct cccggcagtg caaaacccat
      481 ggtggacaag ccgtttgaga tcaagcaggt gttgcgctcg taggtcgggt aaagccgtgc
      541 catccacatt ttggtggcac cggtgtcgac caggacggcg tcgctgcggc ccaacgcctc
      601 gcgggtatcc gcgaccaccc gcgccggcgc cagcggatag cgcgaatcct gttgtccccg
      661 aacgaattcc tcggccagta ggccgcaccc gggaacctcc gtctgaggtt cgaaggcgtg
      721 gcccgcgagc gcgtccgtta gcgcatcgag tgaggcactg atgtcgccga tgatgccgac
      781 gtcaaccggg tagtgcatgt cgacctcggc gggaaaacga tggatgtgga tgatcctctt
      841 gtcggcctgc gggttgatcc ggacggggtc gaattcctgt agctcatacc cgaccgcgat
      901 gacggcgtcg gcgttgtcga aaccgaagtt gacgtagtca tgccgcatga acccgagggt
      961 cccgatgctg ttgggatgat cgtcgggcat cacgcccttg ccgtgaaagg tgttggccac
     1021 cggaatgccc aatgcgtcgg agaaccgcag cagggctttg gtcgcgtcgc cgcgggcggc
     1081 gccatgcccg gcgagcacca ccggtcgttg cgcgttgcgc agaatgtcga ccgcccgcgc
     1141 cacctgccgg ggcgccggcg cttcggcgcg aacgacgttg cggggcaatg gtttcaggtc
     1201 gtagtcggtg gcatcggcgt ctatgtgctc aggcacggcc aggtagaccg cggccggtcg
     1261 ttcggtctcg gcgagcttga acgctttgcg gaacatctcc gggatggcgt gtgcagtggg
     1321 gacgccggcg gcccaccggg tgatcggagc gaacatcgac accaggtcga cgtactggtg
     1381 cgattctttg tactcgcgat cctgaccgac ctgcgcggag atcgcgacca tcggtgtgct
     1441 gttggtcgtg gcgtcggcga ccccgagctg catgttgatc gcgcccggcc ccaaggtggc
     1501 cgacaccacc gccgcccggc cggtgacccg tccatacatc tccgccatga acgccgcggc
     1561 ctgctcgtgc cgggtgagca cataacggac gctcttcgag tgagccaacg cctgaatgaa
     1621 gcggatgttc tcctcgccgg gtattccaaa gacgacggac acaccctcgt tttccaggca
     1681 cttgaccatc agctcggcgg ctttactcat ccggcacctc cctctggaca cgatagtggc
     1741 aggcttgttg gcggacgttt caccagcacc ccgacgaacg cagatgaagg agggccagcg
     1801 tgcccatcgc cacgatcaac ccggccaccg gcgagacagt caagaccttc accccggcaa
     1861 cggacgacga ggttgacgcc gcaatcgcgc gtgcccacga gcggttcctc gactaccgcc
     1921 attcgacgac cttcgcccag cgcgccgaat gggcgaacgc gaccgccgac ctgctggaga
     1981 aggaggccga cgaaaccgcc gccatgatga ccctcgaaat gggtaagacg ctggcctcgg
     2041 ccaaggccga agcgctcaag tgcgccaagg gttttcgtta ctacgccgag aacgcggaga
     2101 agctgctggc cgacgagccc gcggatgcat ccaaggtcgg cgcgaaaaag gcgtacatcc
     2161 gctatcaacc cctcggggtg gtgctggccg tgatgccatg gaactttccg ctgtggcagg
     2221 ccgtccggtt cgccgcgccc gcgttgatgg cgggcaacgt cggcctactc aagcacgctt
     2281 ccaatgtgcc gcagtcggcg ctatatctct ccgacgtgat aacccgcggc ggcttcccgg
     2341 agggctgctt ccagacgctg ctcgtttcgt cgggcgccgt ggagcgcatc ctgcgtgatc
     2401 cccgggtcgt ggcggccacc ctgaccggca gcgaaccggc cggtcaatcg gtggccgcca
     2461 tctgcggcga cgagatcaaa cccaccgtgc tcgaactcgg cggcagcgac ccgttcatcg
     2521 tgatgccgtc cgccgacctc gacgaggccg tcaagaccgc ggtcaccggc cgggtgcaga
     2581 acaacggcca gtcctgtatc gcggccaagc ggttcatcgt ccacaccgac atctacgacg
     2641 cgttcgtcga caagttcgtc gagcggatgg aggggctgaa ggtcggcgac ccgaccgacc
     2701 cggacaccga cgtgggtccg ctggctaccg agtcgggccg cgacgaaatc gcgcagcagg
     2761 tcgacgacgc gatcgcggcg ggcgcgaaga tccgctgcgg cggcacgcgc atcgaccggc
     2821 cggggtggtt ctacccgccg acggtggtca ccgacatcac caaggacatg gcgctctaca
     2881 ccgaggaggt gttcggcccg gtcgcctcga tgtaccgcgc ttccggcgtc gacgaggcga
     2941 tcgagatcgc caacgccacc accttcggcc tggggtccaa cgcctggacc aatgacgagg
     3001 cggagcagga gcgcttcatc gacggcatcg aggccggcca ggtcttcatc aacgggatga
     3061 cggtgtccta ccccgagctg ggtttcggtg gcgtcaagcg gtcgggctac ggccgcgagc
     3121 tggccggcct gggcatccgc gaattctgca acgccaagac ggtctgggtc gggtagctac
     3181 ctcggttggc cgcctaggcc ccgacacccg ccagggcctt ctcgtcctcg tcggcgaagg
     3241 tgtcctccag ctgcagcggc gagtagtcga ggtcgatctc gccgagcggc cggccgcggg
     3301 cactggagat cgtgccgaac cggcgcatcc ccttgtcggc cgcgtactgc atgaactcgt
     3361 ccactgaaag gttgaacggc atcgggtcgt acagggtgaa gccctcttcg atgaggcgca
     3421 gtccgagcgg catgagctcg ttcatgcgcg cttcgaacac cgcccaattg gcgtcgtcgg
     3481 cggcgacgtg gcgccggcag gtgaaggtgc cccatgccat gtggcgtcgc tcgtcgtcac
     3541 cgatgcgccg gaccagctcc tgcatgccgg gaagaatgcc gcgctccacg cagatcttgt
     3601 gccagaggta gtagccggtc agtgccagca tgccttcg
//
LOCUS       DASSGQ010000216         4937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_379784,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000216 DASSGQ010000000
VERSION     DASSGQ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379784"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VFI55_09365"
     CDS             <1..763
                     /locus_tag="VFI55_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7074270.1"
                     /translation="SGRADDAFHVVCPSLPGYGFSGKPTRTGWGVEKIAEAWETLMVR
                     LGYERYGAQGGDWGAAVTTQLGRNRGHCAAIHLNMPIGAPGNLDNPTAEEKAALAELA
                     QHRKWGTGYSKQQSTRPQTLGYGLVDSPVGQLAWIVEKFWAWSDCHGHPENAFGRDEL
                     LDNVMIYWVTGTAASSARLYWESFRVWRGGARVELPTGVAAFPKELLKAPRSWCEPNY
                     NITHWTDMPRGGHFAAFEQPELFVDDLRAFFATVR"
     gene            complement(859..1674)
                     /locus_tag="VFI55_09370"
     CDS             complement(859..1674)
                     /locus_tag="VFI55_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003411520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HET7074271.1"
                     /translation="MKRPATRIADLLNPAALLLPAANVIMQLSLPGVGYGVLESPVDS
                     GNVFKHPIKRARTTGTYLAVAAIGTESDRALIRGAVDVAHRQVRSTSSSPVSYNAFDP
                     KLQLWVASCLYRYFVDQQEFVHGPLDDASADAVYRDAKRLGTTLQVPERMWPPDRAAF
                     DEYWKRTLDELRIDPPVREHLRGVASMAFLPWPLRALAGPFNLFATTGFLAPEFRAMM
                     RLDWSAGRQRRFEWLLAALRLADRLIPHRAWIFSYRLYLWDMRSRARRGRRIV"
     gene            1736..2674
                     /locus_tag="VFI55_09375"
     CDS             1736..2674
                     /locus_tag="VFI55_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020725685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HET7074272.1"
                     /translation="MTPRARIAGVLAGFLADLAVSDPKRGHPVALFGRAAAGVERVTY
                     RDTKVAGALHAGLMVGAVILLGAFAQSAAARRGRAWSVAAAATASWVSLGGTSLVHTG
                     LDMAQLLERGDVEAARQLLPSLCGRDPALLDVPGLTRAALESVAENTSDAQVAPLLWA
                     TVGGVPAVLAYRAINTLDSMIGHHSPRYIRFGWAAARLDDMANYIAARATAALVVLCA
                     PLVGGSPSGAVRAWRRDAARHPSPNAGVVEASFAGALGVRLGGPTQYHHELQIRPTLG
                     DGRPATVADLRRAVRLSRTVQAGAALLVALAHRRRP"
     gene            complement(2656..3501)
                     /locus_tag="VFI55_09380"
     CDS             complement(2656..3501)
                     /locus_tag="VFI55_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HET7074273.1"
                     /translation="MLRFRRGLAFLLRPGWIALALVVVAFTYLCFMVLAPWQLGKNTR
                     TSRENHQIEYSLNTSPVPLKTLLPQQDSSAPDAQWRRVTATGHYLPDVQVLARLRVVE
                     ADQAFEVLAPFVVDDGPTVLVDRGYVRPEPGSHVPPIARPPAQTVTITARLRDSEPTM
                     PGKDPFVRDDVQQVYSINTTQVAALTKVPLTGSYLQLVDSQPGGLGVIGVPHLDAGPF
                     LSYGIQWISFGILAPIGLGYFVYSEIRVRRQEKQAESARDDHQASATPMTVEEKLADR
                     YGRRR"
     gene            complement(3501..3980)
                     /locus_tag="VFI55_09385"
     CDS             complement(3501..3980)
                     /locus_tag="VFI55_09385"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HET7074274.1"
                     /translation="MHVTFVCTGNICRSPMAEKMFADQLRLRGLSDAVRVTSAGTGNW
                     HVGSCADERATRVLRAHGYPTDHRAAQLDADHLTADLVVALGRNHVRMLRQLGVEDSR
                     IRMLRSFDPRSGRYALDVDDPYYGDPEDFEQVLEVIEAALPGLHDWVDERLAQSGHG"
     gene            complement(4042..4728)
                     /locus_tag="VFI55_09390"
     CDS             complement(4042..4728)
                     /locus_tag="VFI55_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET7074275.1"
                     /translation="MTGTASSDCPTPQLVIFDLDGTLTDSAEGIVASFLHALGHVGAA
                     VPEGDLAAQIVGPPMDDTFHSMELGEVAEEAIAAFRTEYAARGWAMNALFDGIEQLLA
                     DLRAAGVRLAVATSKLEPTARRILAHFGLDEHFEVIAGATPDGSRKTKIEVLAHALEQ
                     LQPVPTRVLMVGDRSHDVHGAAAHGIDTVVVGWGYGQADFAHGAAVDGTPAGVTHAAT
                     IDELRRALGV"
BASE COUNT          783 a         1657 c         1703 g          794 t
ORIGIN      
        1 gtccggacgc gccgacgacg cctttcacgt tgtgtgcccg tcacttccgg ggtacgggtt
       61 ttcgggcaag cccacccgca cgggatgggg tgtcgaaaag atcgccgagg cctgggaaac
      121 actcatggtg cgccttggct acgagcggta cggcgcccag ggcggagact ggggcgcggc
      181 ggtgaccacg cagctgggcc gaaaccgggg ccactgcgcg gccattcatc tgaacatgcc
      241 gatcggcgcg ccgggaaacc tcgacaaccc cacggcggag gagaaggccg ccctggccga
      301 gctggcccag caccgtaagt ggggcacggg gtattccaag cagcagtcca cccgacccca
      361 aacactgggg tacgggttgg tcgattcgcc agtgggacaa ctggcgtgga tcgtcgagaa
      421 gttctgggcc tggtcggatt gccatgggca tcccgaaaac gcgttcggcc gagatgagtt
      481 gctcgacaac gtgatgatct actgggtgac cggcaccgcc gcctcctcgg ctcgcttgta
      541 ttgggagagt ttccgggtct ggaggggagg ggcgcgtgtc gaattgccca ccggtgtggc
      601 cgctttcccc aaggagctgt tgaaagcgcc gcgcagctgg tgcgagccga actacaacat
      661 cacgcactgg acagacatgc cccgcggcgg ccacttcgcc gcttttgaac aaccggagct
      721 ctttgtcgac gacttgcgcg cgttctttgc gacggtgcgc tgatactggg cgggcgtcga
      781 atgtgaatcc gacgacgcga cacgcgcggg gtgcgtcgcc ggattcacac tggccggcgg
      841 agtgcgctga cgtccgccct agacgatgcg tcggccccgt cgcgcgcgag atcgcatgtc
      901 ccacaagtaa agccgatagc tgaagatcca ggcccggtgt ggaatcaggc ggtcggcaag
      961 ccgcagggcg gccagcagcc actcgaaccg gcgctgccgg ccggccgacc agtccagccg
     1021 catcatcgcc cggaactccg gcgccaagaa gcccgtcgtc gcgaacaggt tgaacggccc
     1081 cgccagtgct cgtaacggcc acggcagaaa cgccatcgac gcgaccccgc ggagatgctc
     1141 tcgcaccggc gggtcgatgc gtagctcgtc cagggtgcgc ttccagtact cgtcgaacgc
     1201 cgcccggtcc ggcggccaca tgcgctcggg cacctgcagg gtggtcccca accgcttggc
     1261 gtcgcgatag acggcgtcgg cgctggcgtc atccagcggg ccgtgcacga actcctgctg
     1321 atcgacgaag tagcggtaca ggcacgaagc cacccacagc tgcaacttgg gatcaaacgc
     1381 gttgtaggac accgggctcg acgacgtcga tcgaacctgc cggtgcgcga cgtccaccgc
     1441 gccgcggatg agtgcccggt cggactcggt cccgatggcc gcgaccgcca agtaggtccc
     1501 ggtggtgcgg gcccgcttga tcggatgttt gaagacgttg ccgctgtcca ccgggctttc
     1561 cagcacgcca tatccgacgc ccggcaggga cagctgcatg atcacgtttg ccgcgggcaa
     1621 cagcagtgcc gccggattga gcaggtcggc gatcctggtt gcgggccgct tcatcgcgga
     1681 tctcgcctca tgcactcgag tagaccacgt atgagacgct gcgggagtgc ttgccatgac
     1741 accgcgggcc cgcatcgccg gcgtgctggc cggctttctg gccgacctcg cggtgagcga
     1801 cccgaaacgg ggtcacccgg tcgcgttgtt cggccgggcg gccgccgggg tggagcgcgt
     1861 cacctaccgc gacaccaagg tcgccggtgc gctgcacgcg gggctgatgg tcggtgcggt
     1921 gatcctgctg ggtgcgttcg cccagtcggc cgccgcgcgc cgcggccggg cctggtcggt
     1981 cgccgccgcc gccacggcca gctgggtatc gctgggcggg acatcgttgg tccacacggg
     2041 tctggatatg gcgcagctgc tggagcgcgg cgacgtcgag gccgcgcgac agctgttgcc
     2101 gtcactgtgc ggtcgcgatc cggcgctgtt ggacgtcccg ggtctgacgc gcgccgcgct
     2161 ggaatcggtc gcggagaaca cctccgacgc ccaggtggcg ccgctgctgt gggcgacggt
     2221 gggtggagtg cccgcggtgc tggcctaccg cgccatcaac acactggatt cgatgatcgg
     2281 ccaccactct ccccgctaca tccgattcgg gtgggccgca gcacgattgg atgacatggc
     2341 caactacatc gccgcccggg cgaccgcggc gctggtggtg ctgtgcgcgc ctctcgtcgg
     2401 cggttcaccg tcgggcgcgg tgcgcgcctg gcggcgcgat gccgcccggc atcccagccc
     2461 caacgccggt gtcgtcgagg cgtccttcgc cggggcgctg ggtgtccggc tcggcggccc
     2521 cacccagtac caccatgagc tacagatccg gcccaccctc ggtgacggcc gaccggccac
     2581 ggtggccgac ctgcgccgcg cagtccggct gtcgcggacg gtgcaggcgg gcgccgcgct
     2641 attggtcgcg ctggctcacc gtcggcggcc gtagcggtcg gcgagtttct cctcgacggt
     2701 catcggtgtt gcggaagcct gatgatcgtc gcgggccgac tccgcttgct tttcctggcg
     2761 gcggacacgg atctcggagt agacgaaata gcccaacccg atcggcgcga ggatgccgaa
     2821 ggaaatccac tggatgccgt aggacagaaa cggtcccgcg tccaggtgtg gcacgccgat
     2881 cacgcccagt ccgccgggct ggctgtcgac cagttgcaga taggacccgg tcagtgggac
     2941 cttcgtcagc gcggcgacct gcgtggtgtt gatcgaatac acctgctgca catcgtctct
     3001 gacgaacgga tccttaccgg gcatcgtggg ttcggaatct cgcagccgtg ccgtgatggt
     3061 caccgtctgc gccggcgggc gagcgattgg ggggacatga gaccccggct cgggccgcac
     3121 atagccacgg tcgaccagga cggtaggccc gtcgtcgacg acgaacggtg ccagcacctc
     3181 gaacgcctga tccgcctcga ccactcgcaa ccgggccagc acctgaacgt ccggcagata
     3241 gtgcccggtc gccgtcacac ggcgccactg ggcgtccggt gccgacgaat cctgctgtgg
     3301 caacaaggtt ttcaacggca caggcgaggt gttgagagaa tattcgatct ggtggttctc
     3361 ccgcgacgtc ctggtgttct tgcccaactg ccagggcgcg agcaccatga agcacaggta
     3421 ggtgaaggcg acgaccacca gcgccagcgc tatccagccc ggccgcagca ggaaagccaa
     3481 tccccgcctg aaacgcagca tcaaccgtgt ccgctctgcg cgagtcgttc gtcgacccag
     3541 tcatgcagcc cgggcaaggc agcttcgatg acctctaaca cctgttcgaa gtcttcgggg
     3601 tcgccgtaat acgggtcgtc gacatcgagg gcgtatctgc ccgagcgcgg gtcgaatgat
     3661 cgcagcatgc gtatccggct gtcctcgacg cccagctgcc gcagcattcg aacgtggttg
     3721 cggcccaggg ccaccacgag atcggcggtc aggtgatcgg cgtccagctg cgcggcgcgg
     3781 tggtcggtcg ggtagccgtg ggcgcgcaga acgcgggtcg cccgctcgtc ggcgcagcta
     3841 ccaacatgcc agttgccggt gcccgcgctg gtcacccgca cggcatcgct cagaccgcgc
     3901 agccgcagtt ggtctgcgaa catcttctcg gccatcggcg agcggcagat gttgccagtg
     3961 cagacgaaag tgacgtgcaa aggttggccc cgacgacgat gcgggccgtc ggagggcccg
     4021 ctgtggagtg gggcattggc atcagacacc cagcgccctc cgcagctcgt cgatggtcgc
     4081 ggcatgggtc accccggcgg gtgtgccgtc cacggcggca ccgtgagcga aatcggcctg
     4141 cccgtatccc cagccgacca cgacggtgtc gatgccgtgc gcggccgcgc cgtgtacgtc
     4201 gtggctgcgg tcgcccacca tgagcacgcg ggtgggcacc ggctgcagct gttccagcgc
     4261 gtgcgccaac acctcgatct tggttttgcg cgagccgtcg ggggtcgcgc cggcgatgac
     4321 ctcgaaatgc tcgtccagcc cgaaatgggc caggattcgt cgtgccgtcg gctccagctt
     4381 ggaggtggcc acggccagcc ggacaccggc ggcacgcagg tcggcaagca actgctcgat
     4441 gccgtcgaac agcgcattca tcgcccagcc gcgggcggcg tactcggtcc ggaaggccgc
     4501 gatcgcctcc tcggcaacct cgccgagctc catcgagtga aaggtgtcgt ccatgggcgg
     4561 tccgacgatc tgtgcggcca ggtcgccctc gggcaccgcg gcgccgacgt ggccgagcgc
     4621 gtgcaggaag ctggcaacga tcccttccgc ggaatcggtg agcgttccgt cgagatcgaa
     4681 gatcaccagc tgtggagtcg ggcaatcaga ggacgcagtg cctgtcacga caccattgtc
     4741 gtcgatggcg acaccgtggt gtgacggtgt tcctgcctgg ccgcccgcgc ggcccactag
     4801 tgtttcactg atggcgactc tggacccgag cgcgcctgtg gcggcccgct accacggcga
     4861 tcaggccgtc gcccccggga tgctcgattt cgccgtcaac gtccgccacg cgcaaccacc
     4921 gcagtggctg atccggc
//
LOCUS       DASSGQ010000217        10777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_684078,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000217 DASSGQ010000000
VERSION     DASSGQ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10777
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684078"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="VFI55_09395"
     CDS             complement(<1..168)
                     /locus_tag="VFI55_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HET7074276.1"
                     /translation="MDDIAGFVVGAKPADLSGRPRALLKRNVLDSVACAVGSLDGELI
                     TTIREHTEQFSG"
     gene            complement(269..1129)
                     /locus_tag="VFI55_09400"
     CDS             complement(269..1129)
                     /locus_tag="VFI55_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7074277.1"
                     /translation="MLDIVELLARSANTRLRFSDIVRELDLTQATAHAILKTLCDRGW
                     ASRDPVAKTFTLGPALAVVAARTDTVRPLAHAARSAALRLSREVGFAGSVVERFGDSL
                     VVTAFEGDPATQPAGIPGDRIPYAPPFGVALAAWDSEEERRAWIRRGAGNNTDRIARL
                     ERVLEHTRERGFDVDWTTPALAQAVQVVGTLDSNEMPTPVRHIVDQLLVEFTTIGFLS
                     DDNPGRRKQPVVTIAAPVFDHRRQVALMVAVHPLCPLSARQIRVIGKKLTDETAAITK
                     SQRHAVNGAV"
     gene            1254..2306
                     /locus_tag="VFI55_09405"
     CDS             1254..2306
                     /locus_tag="VFI55_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7074278.1"
                     /translation="MRAVVLRDGRLQVRDTPDPVPGPGELLLRTLSTAICASDVHFMD
                     HPELAVEDPTGRSLYDADRDIVLGHEFVGEVVARGPGCTDQLALGSRVTAMPVRLVDG
                     GAGGLRIIGQHPEAQGSFGELLVVSEAAAKPVPGDVSNDAIALTDAFAVGEFYVRSAQ
                     IQPGEIPIVIGAGAIGLSAVAALAGRGVEPIVVADYKPDRRELARDRFGAHVVVDAAE
                     KSPFDAFREVRAERGLPGPAVVFECVGAAGLIQSIVESAEMATRIYCAGGWYTGDTLD
                     ITTATRQGVTIQFGGGPHPQDWYGTLDAIASGRLDPLPSVGKVIGLDEVPAALDLARR
                     SEGPPRIVVHPNGDVP"
     gene            2303..2716
                     /locus_tag="VFI55_09410"
     CDS             2303..2716
                     /locus_tag="VFI55_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7074279.1"
                     /translation="MTDRDDRQDIFDLLVRYATGIDRRNWPLFRTVFTDDCQLDYGEI
                     GAWNGIDAVAQFMEQVHALAGHTMHRLSNQAIAVDGDNATARTYIDGLIMAGDNESGV
                     NAIGFYDDAIVRTDDGWRIARRRYTQVRLTTVGET"
     gene            2726..3544
                     /locus_tag="VFI55_09415"
     CDS             2726..3544
                     /locus_tag="VFI55_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7074280.1"
                     /translation="MTFAAKYGPWALVAGASDGLGAAFAAGLAERGVNVVLLARRQAA
                     LDQIAAQIDSQTSAQTRTLAMDLAEPDAASAIAAATSDLEIGFLVYCAGADPSFTPFL
                     ANPIEAAEAMVQRNCMVPMQLCHHFAPAMVGRGRGGIVIFGSGAGLAGGPNMVAYGAS
                     KAFDMVFAEALWAELHDKGVDVLGLILGKTDTPALRQLEYTRGQISKPDDAPPGAAAV
                     ADVIAEAFENLTNGPTWIVGEDMRAAVQLMASLTRNQVVELMTQASATAMGPTD"
     gene            4034..4333
                     /locus_tag="VFI55_09420"
     CDS             4034..4333
                     /locus_tag="VFI55_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074281.1"
                     /translation="MTTSLTPPAWIEPDSPPRWLRLDDGRMVASWERIVSAAVWIARE
                     DYLSPDGQTLPGDTTIHVEQRLDGMAPDAVRELATALLDAAWLVENGALSSTEAA"
     gene            5244..6542
                     /locus_tag="VFI55_09425"
     CDS             5244..6542
                     /locus_tag="VFI55_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HET7074282.1"
                     /translation="MLAIDRPAAAVSTRSRARWRTLTRSALITTIFIAPAVVVRALGL
                     HVDPVAALLIFGGAVVAASFVLAWAAEAAQIDVSGGLAIAGLALVAVLPEYAVDLYYA
                     YVSGHNAEYTQYAAANMTGSNRLLMGLGWPVVVLVGSVVARNAGADQRGGLALEPANR
                     VELGFLLIAGVVAFVIPASGEIHLALGLALLAWFGFYLYKVSHGDVEEPDLIGTAAAL
                     GELPDRSRRIAVAGLFAVSGAVILLCAKPFADNLVAAGTELGIDRFLLVQWLAPLASE
                     APEFIIAIIFASRGKGTAAMATLISSKVNQWTLLVGSLPIAHALGGGGLSLVLDSRQT
                     EEVLLTATQTLMGVALILALRFRRAAAWALLVLFAVQFPIVSTQGRLLLCGIYTAVAI
                     AGLIVNRRYLLSTVRAPFVGRTIRHSGRPQYAEDDPGPRT"
     gene            6546..7391
                     /locus_tag="VFI55_09430"
     CDS             6546..7391
                     /locus_tag="VFI55_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7074283.1"
                     /translation="MAHAVLTIIGVGGMGHAIARRLGAGMTVLLADYNEAALASASGS
                     LSAEGHHVESRVVDVSSPESVRELAEHAAALGAVTRVAHTAGLSPSQASAEAILAVDL
                     LGVALVLDAFGEIIAPGGAGVVIASMAGHLFPPLTAAQEHALAHTPTGELMGLDFVSP
                     RRITEPGIAYGIAKQANHIRVRAASAQWGRRGARINSISPGIISTPMGQQELASPIGD
                     GMRAMIAASATGRLGTPDDIASAAAFLLGHDASFVTGTDLLVDGGVIAAIKVGSPEAQ
                     APQTR"
     gene            complement(7379..9163)
                     /locus_tag="VFI55_09435"
     CDS             complement(7379..9163)
                     /locus_tag="VFI55_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HET7074284.1"
                     /translation="MRLALNYDLLKGRHDLVAGLTVAAISFPQAMAYALIAGVDPRFG
                     VYSAIVVTIVASVFGSSSHLINGPTSAISLLVFAALAFIDSENTHELFEGLFLLAVLV
                     GAFQILISLLKLGKLTRYISESVIIGFMAAAAFLLAVGQLGNALGVRDKGNGHMQVLR
                     RVWLTLFHGDHVNYRAVILSGTAVALAVVLRKLVQRYGWPQIDMLAVLIITAVIAYVA
                     GWSIPGPNGHTAVAVAAKIPRSLPTPHIPEIHTGWLPELSELSTGALAIAFVGIIEAL
                     SIAKAIAYQTQQKIDYNRQIMAEGLANLAGGFFQSMPGSGSLSRSAINFQAGGKTRFS
                     GIVAAATVTAALLLFAPLLRYVPQPALAGLLLVTAARLVDFKRLVYTLKASRYDAGLV
                     VVTAFTGVAIDLDKAVLLGVVLSIVLFVPRAARLKAKELIVTPERVVRERIPGDSADP
                     SIIICDLEGELFFGAATELDRYLAEISERIADDQVKFVVLRLKRVRHPDVVCIERIEH
                     FLRAQEADGVTVLLAGVRPDTLAVLTNVGFQSWFPTDHVFPEEDEEYSATLRAVRYAN
                     NKLAELQLREPITTASEITSHTDLYYRV"
     gene            complement(9181..10260)
                     /locus_tag="VFI55_09440"
     CDS             complement(9181..10260)
                     /locus_tag="VFI55_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7074285.1"
                     /translation="MVETPPPVNRARIKWRIPWLNVLGVVAIVVATTAIVVNNLPRDE
                     SNQILNVSYDPTRELYAAIDKAFVPQYRTHSGITLDIKESHGGSGRQLRSVLDGTQKA
                     SVVSLALISDIQTLSKRGFIAPDWRQRLPNGSVPYTSTIVFVVRKGNPKAIHDWPDLV
                     KGDVSVVTPNPRTSGNGQLSVLAAWGSVTTRGGNQADAIAYLRSLFHHVAVSDAGARG
                     AANSFALAKIGDVQLTWENEALREVGANKDELQLVYPPVSILAEPAVAWVDANVTDPR
                     TATDAKAYLNYLFTDAAQEQVAQFGYRPFKPEILARHADRLPPLALFPISAIAKDWSD
                     AREQFFGTNGILDTISAAPAAASGT"
     gene            10454..>10777
                     /locus_tag="VFI55_09445"
     CDS             10454..>10777
                     /locus_tag="VFI55_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HET7074286.1"
                     /translation="MAQRALRERLIDLEVPSEDVARGRALRDAAPRRSLAQLTPSNRT
                     ATEILVAQNADRLHELVPLRFARMLTDPFSFYRGAAAVMAADLASSPTTGIEIMCCGD
                     AHISNF"
BASE COUNT         1859 a         3539 c         3517 g         1862 t
ORIGIN      
        1 gccgctgaac tgctcggtgt gttcgcgaat cgtcgtgatg agttcaccgt ccagcgagcc
       61 gacggcgcag gcaacgctgt ccagcacgtt gcgtttcagc aatgcccggg gccgcccgct
      121 caggtccgcc ggcttggcgc cgacgacgaa gccggcaata tcgtccacaa cgtagttgtc
      181 cgccacggct tttcgtcctt ccgtcgatcg cgctccaagg gagattatct gtcgcctcga
      241 ggccgagccg gtagccgtgt aggcacggtc aaacggcccc attaaccgca tgccgctgcg
      301 acttggtgat cgcggccgtc tcgtcggtga gtttcttccc gatgacccgg atctgccgcg
      361 cgctgagcgg gcacagcgga tgaacggcca ccatcagggc gacttgtcga cgatggtcga
      421 aaaccggcgc ggcgatggtg accacgggct gtttgcggcg acctgggttg tcgtcggaga
      481 ggaagccgat ggtggtgaac tcgacgagca actggtccac gatgtgccgc accggagtcg
      541 gcatctcgtt gctgtccagc gtgccgacca cttggacggc ctgcgccagg gccggcgttg
      601 tccagtcgac gtcgaatccg cgctcacgtg tgtgctccaa cacgcgttcg aggcgcgcga
      661 tgcgatcggt gttgttgccc gcaccgcgcc ggatccacgc ccgtcgctcc tcctcggaat
      721 cccaggcggc gagtgcgacg ccgaacggag gggcataggg aatgcgatcg cccggtattc
      781 ccgccggctg ggtagcggga tcgccctcga aggcggtgac caccaatgaa tcaccgaatc
      841 gctcgacgac agacccggcg aagccgacct cgcgggacag ccgcagggcc gccgaacgag
      901 ccgcgtgcgc aagcgggcga acggtgtcgg tccgcgcggc caccaccgcc agcgcggggc
      961 cgagggtaaa ggtcttcgcg acgggatcgc ggctggccca tccgcgatca cacagcgtct
     1021 tgaggatcgc gtgtgccgtc gcttgagtga gatccagctc gcgcacgatg tcggagaacc
     1081 gcagccgcgt atttgcggac cgggccagga gctcgacgat gtcgagcacc cgggcggtcg
     1141 gcgccgacgt cgatccgcga atcgcttgac tcctccgtga cggcgttcct acagttacca
     1201 cgagccttcg agattttata tcggttattc gagaattaca cggggctgac gcattgcgcg
     1261 ctgttgtgtt gagagacggc cgactacagg tccgcgatac accggacccc gtgcccggcc
     1321 cgggtgaact gctgctgcga acgctgagta ccgcgatctg cgcatccgac gtgcacttca
     1381 tggatcaccc cgaactggcc gtcgaggatc ccaccggtcg ctcgctgtat gacgcggacc
     1441 gtgacatcgt gctcggccac gagttcgtcg gtgaggtcgt cgcgcgcgga cccggctgca
     1501 ccgatcagct cgccttgggt tcgcgggtga cggcgatgcc cgtgcgactt gtcgacggcg
     1561 gcgccggcgg gctgcgcatc atcggccagc acccggaagc ccagggcagt ttcggcgagc
     1621 tgctggtggt ttcggaagcg gcggccaaac cggtccccgg cgacgtctcc aatgacgcca
     1681 tcgcgctgac cgatgccttc gcggttggcg agttctacgt gcggtcggcg cagatccaac
     1741 cgggcgagat ccccatcgtc atcggcgccg gggcgatcgg cctgtcggcc gtcgccgccc
     1801 tggccggccg cggcgtcgaa ccgatcgtcg tggccgacta caagcccgac cgccgcgagc
     1861 tggcccgtga tcgcttcggc gcccacgtcg tcgtcgatgc cgccgagaag tcaccgttcg
     1921 atgccttccg ggaggttcgc gccgaacgcg gattgccggg cccggctgtg gtattcgagt
     1981 gcgtcggcgc cgccgggctc atccagagca tcgtcgaatc cgccgaaatg gctacccgga
     2041 tctattgcgc gggtggctgg tacaccggcg acacgctgga catcaccacc gccacccgcc
     2101 aaggcgtcac catccaattc ggcggcgggc cacacccaca ggactggtat ggaacgctcg
     2161 acgccatcgc gtcgggacgg ctggacccgc tacccagcgt cggcaaggtc atcggcctcg
     2221 acgaagtccc cgccgcactc gacctcgcgc gcaggtctga gggcccaccg cgcatcgtcg
     2281 tccacccgaa cggagacgtc ccatgaccga tcgagacgac cgccaagaca tcttcgacct
     2341 gctggtgcgc tacgccaccg gcatcgaccg acgcaactgg ccgctgttcc gaaccgtgtt
     2401 caccgacgac tgccaactcg actacggcga gatcggcgca tggaacggca tcgacgccgt
     2461 cgcacagttc atggagcagg tgcatgccct ggcgggacac acgatgcacc ggctgagcaa
     2521 ccaggcgatc gcggtagacg gcgacaacgc caccgcccgc acctatatcg acgggctgat
     2581 catggccggt gacaacgaat ccggcgtcaa cgctatcggt ttctatgacg acgcgatcgt
     2641 tcgaaccgac gatgggtggc gcatcgcgcg ccgcaggtat acccaggtgc ggctgaccac
     2701 cgtcggtgaa acataaggag tatcggtgac attcgcagcc aagtatggac cgtgggcgct
     2761 ggtcgccgga gcttccgacg gcctgggcgc cgccttcgcc gcggggctcg ccgagcgcgg
     2821 cgtcaatgtg gtgttgctgg cccgtcggca ggcggcgctg gatcagattg cggcacagat
     2881 cgattcgcaa acatcggccc aaacacgcac tctggcaatg gacctcgcgg aacccgacgc
     2941 ggcgtcggcg atcgccgcgg caaccagcga cctggagatc gggttcctcg tgtactgcgc
     3001 gggtgccgac ccgagcttca cgccgttcct ggccaatccg atcgaggccg ccgaagcgat
     3061 ggtgcaacgc aactgcatgg tgccgatgca gctctgccac cacttcgccc ccgccatggt
     3121 cgggcggggc aggggcggaa tcgtcatttt cgggtctggc gccggattgg ccggggggcc
     3181 taacatggtc gcctacggcg cctccaaggc gttcgacatg gtcttcgccg aagcgctgtg
     3241 ggccgaactg cacgacaagg gcgtcgacgt cctgggcctc atcttgggca agaccgatac
     3301 gcccgcattg cggcaactcg aatacacccg cggccagatc agcaagccgg acgacgcgcc
     3361 gccgggcgcc gcggccgttg ccgatgtcat cgccgaggcg ttcgagaatc tgaccaatgg
     3421 gccgacctgg atcgtgggcg aagacatgcg cgccgccgtg caactgatgg cgtcgctgac
     3481 ccgcaatcag gtcgtcgaac tgatgacgca agccagcgcc acggcgatgg gccccaccga
     3541 ttgatctgga ttcgattgac ggcgccgcac gcaatccgat ttgccaagtg ccacaatttg
     3601 tttcggagcg atggtttgga acccgatcgc cgctggcgtt ccagcggggc gcaccagacc
     3661 gacgagccgc atgaggccac cgtagcgcta caccagctgc tggttgatac gctcctggcc
     3721 acgcgcagcg aggtgattgt gctcaaagcg attagaacgg gtccggagaa tccccaggta
     3781 ccggagaatg tttgtgatcg gggagcctgt ggccgacgac caagtgtgtc tcgaagatcg
     3841 ttacgccctg ggtgttgtcg tggcagtaga ggtgacgacg ctcacagcgt gcggtgaact
     3901 gttagagatt tcacacttga tggcaacgtc ggtaacaggg ccgtgattga aatgccttgg
     3961 gaatgtgcat cgcacaaggt gagagcgttg aactggcttg ccgtgcagcg gaattgacgc
     4021 tagcgtcagc gctatgacga catcactaac acctcccgct tggattgaac ctgactcccc
     4081 gccgcgctgg ctgcgcctcg acgatggccg catggtcgct tcatgggagc gcatcgtgtc
     4141 agccgccgtg tggatcgccc gtgaggacta cctcagcccc gacggccaga cactgccggg
     4201 cgatacgacc attcacgtcg agcagcgcct cgacggcatg gcgccggacg cggtgcggga
     4261 gctagccacc gcgctgcttg acgccgcttg gctggtcgag aacggggcgc tcagctcaac
     4321 ggaggcggcg taacgccccg tgaccggtgg aatgggacgg accgaatagt agaaggacca
     4381 acaggcgacg atgcctcgcg gccttcctcg ttggctgcgc tggccgccac tgagtcccgc
     4441 tagccgcgca cggctctctc gacatcttcg cgcgtcaggc tgaagtgttc taccgctcac
     4501 ccgagatgct tactacgccg accgacggtg gccacatagc agtaaccagc tacatatccg
     4561 gtcggccgat gtctgcgaca cgtccgagaa gattgccttg ccgatatcga tgaagccacc
     4621 cccggtgtcc tccttggaca tcttcttgat tgccgcatga atccagcaca ctgcgtcacg
     4681 cgtcccggtg ccgtgaagtt gcctacctgc tgtagaaggt ttcaaagcgc tgcccaagct
     4741 cctagacgag gggctcgtgg ggccgctctg aaagaacacc gtgagatgag gcgcatcgcc
     4801 agcctcgatt cttcgccgct cggcgatgcc aaaaacgggc attcgcctgg gtacgcgtcg
     4861 tagcaatcat ggattaggac gttcccggtt gtccggaggt cggcactccc ccgaaaccaa
     4921 ccgagctacc cctgcatccg tcgcggcggt ggatcaaacc gggatgccag gccacccggt
     4981 cgccttccga gtcgtatcgc catcggtcca aaacgccatc gcacggcagt aaaaggtttg
     5041 ccaaagtttc agataagcac gtccgaactt tcggcatcgc cgcatgtggc cagcaaatac
     5101 agggtccggc acgctgtcgc tttgcccgcc cacaacgttg ctggccacgg gtttcgcggg
     5161 taccttggga cggcagtctc gacagcaagc gagccgacgt aatcacccgt gatcggcacc
     5221 cctcgatgcg ctgaaggaag acgatgctcg ccatagacag gccggctgcc gcggtctcga
     5281 cgagatcgcg tgcgcgttgg cgaaccctga cccgttcggc gctgatcacc acgatcttta
     5341 tcgccccggc ggtcgtggtc cgggccctcg gcctccatgt cgaccccgtc gccgccctct
     5401 tgatcttcgg cggcgcggtc gtggcggcca gcttcgtgct ggcgtgggcc gccgaagccg
     5461 cgcagatcga tgtctcaggc ggcctggcga tcgcggggct cgcattggtc gcggtgctgc
     5521 ccgaatatgc cgtcgacctc tactacgcgt atgtgtccgg ccataacgcg gagtacaccc
     5581 agtacgccgc cgccaatatg accgggtcca atcggctctt gatgggcttg ggctggccgg
     5641 tcgtggtgct ggtcggcagc gtcgtggcgc gaaatgccgg tgccgaccag cgcggcggtt
     5701 tggcgctgga gcccgccaac cgcgtcgaac tcgggttcct gctgatcgcc ggcgtggtcg
     5761 ccttcgtcat cccggcgagc ggcgaaatcc acctggctct tgggctcgcg ctgctggcct
     5821 ggttcggctt ctacctctac aaggtcagcc acggcgacgt ggaggagcct gacctcatcg
     5881 gtaccgccgc cgcgctggga gaactacccg atcgcagccg ccgcatcgcc gtcgccggtc
     5941 tcttcgccgt gtcgggcgcg gtgatcctgc tgtgcgccaa gccgttcgcc gacaatctgg
     6001 tcgcagccgg caccgaactg gggatcgacc ggttcctgct ggttcagtgg cttgccccgc
     6061 tggcctcgga agcccccgag ttcatcatcg cgatcatctt cgcctcccgc ggcaagggca
     6121 cggcggcaat ggccacgctg atctcgtcca aagtgaacca gtggacgctg ctggtcgggt
     6181 cgttgccgat agcccacgcg ctcggcggcg gcggattgtc cctggtgctc gactcccgcc
     6241 agacagagga agtgctgctc accgccaccc agacgctgat gggggtggcg ctgatcctgg
     6301 cgctacgttt tcgccgggcc gccgcgtggg cgctgctggt gttgttcgcc gtccagttcc
     6361 cgatcgtctc gacgcaaggc cggctgctgc tctgcgggat ctacacggcg gtcgcgatcg
     6421 ccgggctgat cgtcaaccgc cgctacctgc tctccaccgt cagggcgccg tttgtcggca
     6481 ggacgatccg gcacagcggt cgcccacagt acgcggaaga cgatcccggc cccaggacat
     6541 aaccggtggc tcacgcagtg ttgaccatca tcggcgtggg cggcatgggc cacgcgatcg
     6601 cccgccggct gggcgcgggc atgaccgtcc tgttggcgga ctacaacgag gcggcactgg
     6661 cgtcggcgtc cggttcgctg tccgcggagg gccatcacgt cgaaagccgc gtggtcgatg
     6721 tctcgtcgcc ggaatcggtg cgcgagcttg ccgaacacgc ggccgcactc ggggccgtca
     6781 cacgggtggc acacaccgcc ggcctgtcgc cgtcgcaggc ctcagcggaa gcgatcctgg
     6841 ccgtggatct gctcggcgtc gcgctggtcc tggacgcatt cggcgagatc atcgcgccgg
     6901 gcggcgcggg cgtcgtgatc gccagcatgg ccggacactt gttccccccg ttgaccgccg
     6961 cgcaggaaca cgcgctggcc cataccccta ccggcgaact gatgggactg gacttcgtca
     7021 gcccccgacg catcaccgag cccggcattg cctacggaat cgccaagcag gccaaccaca
     7081 tccgagtacg tgccgccagc gcacagtggg gccgccgggg tgcacgcatc aactcgatca
     7141 gtcccggcat catctctacg ccgatgggac agcaggaact tgcctccccg atcggtgacg
     7201 gcatgcgggc gatgatcgcc gcgtcggcaa ccggacggct gggaactccg gatgacatcg
     7261 cctcggccgc ggcgttcctg ctcggccacg atgcatcgtt cgtcaccggt accgacctgc
     7321 tcgtcgacgg aggcgtcatc gcggccatca aggtcggaag ccccgaggcg caggcccctc
     7381 agacgcggta gtagaggtct gtgtgggagg tgatttcgga tgcggtcgtg atcggttccc
     7441 ttagttgcag ctcggccagc ttgttgttgg cgtagcgcac tgcccgaagc gtcgccgagt
     7501 attcctcgtc ctcttcgggg aagacgtggt cggtgggaaa ccagctttgg aatccgacat
     7561 ttgtcaaaac ggcgagcgtg tcgggacgca ccccggcgag cagcacggtc accccgtccg
     7621 cctcctgtgc gcgcaggaag tgctcgatgc gctcgatgca cacgacgtcc gggtggcgca
     7681 cccgcttgag ccgcaacacc acgaacttga cctggtcgtc ggcgatccgc tcgctgatct
     7741 ccgccaggta gcggtccaat tccgttgcgg ctccgaagaa cagctcgcct tccagatcgc
     7801 agatgatgat cgagggatcc gccgagtcac ccggaatgcg ttcgcggacc acacgttcgg
     7861 gcgtcacgat gagttctttg gctttcagcc tggccgcacg cggcacgaac aacacgatcg
     7921 acaggaccac gcccagcagc accgctttgt cgagatcgat cgccacaccg gtgaatgcgg
     7981 tgacgaccac caaacccgcg tcatagcggg acgctttgag cgtatagacc agacgcttga
     8041 agtcgaccag ccgcgcagcc gtcacgagca agagcccggc cagcgccggc tgcggaacgt
     8101 agcgcagcaa cggggcgaac agcagcaacg cagcggtgac cgtcgctgcc gcgacgatcc
     8161 cggagaatcg tgtcttgcca ccggcttgga agttgatcgc cgaccgtgac agcgacccgg
     8221 agccgggcat gctctggaag aagccgccgg cgaggttggc caagccttcg gccatgatct
     8281 gccggttgta gtcgatcttc tgctgggtct ggtaggcaat ggctttggcg atcgacaacg
     8341 cttcgatgat gccgacgaag gcgatggcca gcgcccccgt agaaagctca gacagctcgg
     8401 gcagccagcc ggtgtggatt tcgggaatat gcggggtggg caagctccgc gggattttcg
     8461 ccgccaccgc caccgccgta tgcccgttgg gccccggaat cgaccagccc gcgacatagg
     8521 ctatgacggc ggtgatgatc agcaccgcaa gcatgtcgat ctgcggccat ccataccgct
     8581 gcaccagctt gcgcaaaacg accgccagcg ccaccgctgt gccactgaga atcacggcac
     8641 ggtagttgac gtgatcgccg tggaacaagg tgagccacac gcggcgcaag acctgcatgt
     8701 gaccgttgcc cttgtcgcgc acgcccagcg cgttgcccaa ctgtccgacc gcgagcagga
     8761 aggccgcggc ggccatgaag ccgatgatga ccgactcgga aatgtagcgg gtcagtttgc
     8821 ccagcttgag gaggctgatc aggatctgaa acgcgccgac gaggacggcc agcaggaaca
     8881 gcccctcgaa gagttcgtgc gtgttctccg aatcgatgaa ggcaagggcg gcgaacacca
     8941 gcagcgagat cgcgcttgtc ggcccgttga tcagatgcga ggacgatccg aagaccgagg
     9001 cgacgatggt caccacgatc gccgaataca caccgaagcg gggatccacc ccggcgatca
     9061 gcgcgtacgc catcgcctgg ggaaacgaaa tcgccgcgac ggtgagaccg gcaacgagat
     9121 cgtgccgtcc cttcagcagg tcgtaattca gagcgaggcg cactttgtca cgtcctccgg
     9181 ctatgttccc gacgccgcgg cgggcgccgc cgagatcgtg tcgaggattc cgttggtacc
     9241 gaagaactgc tcgcgcgcgt cgctccagtc tttggcaatg gcgctgatcg ggaacagcgc
     9301 gagcggaggc agccggtcgg cgtgtctcgc caggatttct ggcttgaacg gccgatagcc
     9361 gaattgcgcc acctgctcct gcgcggcgtc ggtgaacagg tagttcaggt aggccttggc
     9421 gtcggtggcc gtcctgggat cggtgacgtt cgcgtcgacc caggccaccg cgggctcggc
     9481 gaggatgctg accggcgggt agacgagctg aagttcgtcc ttgttggcgc cgacctcgcg
     9541 cagcgcctcg ttctcccagg tcagctgaac gtcgccgatc ttcgccagcg cgaagctgtt
     9601 cgccgcgccg cgggcgccgg cgtccgagac cgccacgtgg tggaacaggg accgcaggta
     9661 ggcgatcgcg tcggcctggt tgccgccccg cgtggtgacc gagccccagg ccgcgagcac
     9721 gctcaactgg ccgttgcccg aggtgcgcgg attgggtgtc accaccgaca cgtcgccttt
     9781 gacaagatcg ggccagtcgt ggatggcttt gggattgccc ttccggacaa cgaacacgat
     9841 ggtcgacgtg taaggcaccg aaccgttcgg cagccgctgc cgccagtcgg gcgcgatgaa
     9901 tccccgcttg ctcaacgttt ggatgtcact gatcagcgcc agcgacacca cgctggcttt
     9961 ctgggtgccg tccaggacgc tgcgtagctg acgccctgag ccgccgtggg actccttgat
    10021 gtccagcgtg atcccggagt gcgtgcgata ttgcgggacg aacgccttgt cgatcgccgc
    10081 atacaactcg cgtgtcgggt cgtaggacac gttgaggatt tggttggact cgtcacgggg
    10141 aaggttgttg accacgatcg ccgtggttgc cacgacgatc gccaccacac cgagaacgtt
    10201 gagccaggga atgcgccact tgatgcgggc acggttaact ggcggaggcg tttctaccat
    10261 tggcaggcgt cctctcggag atctcgcagt tgatcgggcc gggcatggtc tgcccgcgcg
    10321 cattttcaac gatgttaacc gccaggcaac ctcgcgacag cgttgagaat cagtctgacg
    10381 ataaatacat ctgccgaaat cggggccgtc gaaagccccg cggggttacg tcgcagcaaa
    10441 ctatgctgac cgaatggcgc agcgggcgtt gcgtgaacgg ttgattgacc tcgaggtacc
    10501 gagcgaggac gtggcccgtg gtcgggcact gcgtgacgcc gctccacgtc gttccctcgc
    10561 gcaactgacg ccctccaacc ggacggccac ggaaatcctt gtcgcgcaga acgccgaccg
    10621 actccacgag ctcgttccgc tgcgttttgc ccggatgctc accgacccct tctcgttcta
    10681 ccggggcgct gcggcggtca tggcggccga cctcgcctcg agcccgacga ccgggatcga
    10741 gatcatgtgc tgcggagatg cccacatctc gaatttc
//
LOCUS       DASSGQ010000218        14867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1260893,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000218 DASSGQ010000000
VERSION     DASSGQ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14867
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260893"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VFI55_09450"
     CDS             complement(<1..495)
                     /locus_tag="VFI55_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074287.1"
                     /translation="MRIAALIAVSLLIAGCSDTGNGNSGQPQTSGPSAGPSSAGKPSV
                     PSSAPPAGAAISDVIAWIEAGHPADPGRYHGVTRDGLTTQLGKDIAFSAAGGAGRPVV
                     CMTDSKHTGGALACLVDLANPPPRPETAYGEWKAGWVDFDGTSLQIGAARGDPGPFVN
                     GDGAQ"
     gene            576..2093
                     /locus_tag="VFI55_09455"
     CDS             576..2093
                     /locus_tag="VFI55_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HET7074288.1"
                     /translation="MHERTVRARTTTGTVEGFTRDGVNRWRSIPYARAPLGPLRFRAP
                     QPAQPWSGVRHCHGFTNCAPQQRMYTVLGLSGFGGKYQPMSEDCLTLNVVTPEAAAAE
                     PLPVMVFIHGGGYILGSSATPVYDGAALARRGCVYVSVNYRLGALGCLDLSSLSSPDI
                     AIDSNLYLRDLVLALRWVRDNIAGFGGDPANVTIFGESAGACITATLLAVPAAEGLFA
                     RAISESPASGLVRSKDIAAEFATRFAQLLGVRAHDAAQALMRASTAQLVETQHRLIDQ
                     GTEDRLGAFPIGPVIGDDMLPLDPVEAMRDGRAHRVPLIVGTNAEEGRLFTRFLKMLP
                     TNRSMIEELLADTEPAARERITAAYPDYPAPSACIQLGGDFAFASAAWQIAEAHGTHA
                     PAYLYRYDYAPRTLRWSGLGATHGTELLAVFDIYRTRFGALLTAAADRRAALRVSNQI
                     QRRWRSFSRTGSPGEDWPAYTPAGRAVMVFDTKSHVEFDPHPQRRMAWDGFSLAR"
     gene            2112..4121
                     /locus_tag="VFI55_09460"
     CDS             2112..4121
                     /locus_tag="VFI55_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET7074289.1"
                     /translation="MHADSEQIIERPGDLTAAWLTAAIGAGPVADFAVERIGTGQMSE
                     CYRIRLHYADARSDGPESVVLKVAATDPVSRQTGSALGLYEREVRFYGDIAPRLAGPI
                     APCYQAAIDTSTGVFDLLLGDAGPAVVGDEITGATIEQARLGVVELGRLQGPLLGDTA
                     LAEASWLNRETALNQAMIASLYAGFLDRYGDQIAPEHRVVCERLIAAFDGYVEAEARP
                     NRIQGLVHGDYRLDNMLFGTAGADRALTVVDWQTVSRGPALTDLSYFLGCALRTQDRR
                     DHYDALLRAYHEALGPEAPIGLAGVAEGVRRQSFFGVMMAIVSSMLVERTERGDRMFM
                     TMLQRNCDHALDTEALTTLPVAVATPPPRPAADDELAHAPTDEPLWSESWYADFIDAA
                     QGLGGWFRVGLIANQQTAWVHALLCGPVMPTVAVVDPDVRLPPEPWRVPADALEVSHS
                     VTAPLQTYRVDVRARGRAYPDPSALLRGEAGNPVDVTMNLVWATDGTPYKYRLTTRYE
                     IPCTVSGTVTVDDTSYRVDSVPGQRDHSWGVRDWWSMDWIWSALHLDDGTHLHGVNIR
                     IPGAPTFSVGYVQEADGPHVNVTELQTVDSREAFGTNGLPLQATLIFQPGAIAADVDV
                     RGHAPVRLTAADGRVSQFPRAWAAISTADGRTGVGWVEWNRNLAE"
     gene            4166..4654
                     /locus_tag="VFI55_09465"
     CDS             4166..4654
                     /locus_tag="VFI55_09465"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HET7074290.1"
                     /translation="MGVSGSGKSTVGAALAQRLGVPFVDADVLHPPVNIAKMAAGEPL
                     NDDDRYPWLEKVGEWLAGHRNGGVASCSALKRKYRDQLRAHCPRVEFLYLAGSPELIA
                     GRLAARSGHFMPATLLRSQFDALEPLGADEAGVTVDAGQDVGAIVDAYVAGSAARPGR
                     DA"
     gene            4658..5053
                     /locus_tag="VFI55_09470"
     CDS             4658..5053
                     /locus_tag="VFI55_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003902241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HET7074291.1"
                     /translation="MYVIVVVLVVAVHFAFIGYVVVGGFLALRWRRTIWLHVPAVIWG
                     IAIATNRIDCPLTWAERWARRNAGMAPLPPDGFIAHYITGVMYPAGWAGGVRLSVLAL
                     VALSWAIYVRLGVGRRRRAITRGACDDES"
     gene            complement(5022..5888)
                     /locus_tag="VFI55_09475"
     CDS             complement(5022..5888)
                     /locus_tag="VFI55_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7074292.1"
                     /translation="MLIRRIARPLLSAAFVGQGVDSLLNPKSAAEAAAPTVDGLRALP
                     DPLGSSIPTDAQTFAQLNAAVQIGGGLLLATGKAPRIASAALAFTVLPANLGAHMFWS
                     ESDPQIKAEKRREFLTDLSLLGGLMIASADTAGKPSLGWRGRRAAERLSERVSSALPG
                     SDDTFEAELGELGEKIVQGLQIGAERGRELASTAAERGAPYAEAALERGRELASTAAE
                     KSAPLARKARIRGEELADTARVRGAELAESARLHGSQFADTAVARGSKLADAAREKVG
                     DGARTRRRRRPW"
     gene            5963..6523
                     /gene="pncA"
                     /locus_tag="VFI55_09480"
     CDS             5963..6523
                     /gene="pncA"
                     /locus_tag="VFI55_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrazinamidase PncA"
                     /protein_id="HET7074293.1"
                     /translation="MRALIIVDVQNDFCEGGSVPVTGGTALASAINDYLAGEPGYHHV
                     VATEDYHIDPVDHFSERPDFASSWPPHCVAGSPGADFQPDLDTRRIEAVFRKGAYAAA
                     YSGFEGVDENGTSLLEWLRQRGVDQVDVVGIATDYCVRRTAEDAARAGLTTRVLVDLT
                     AAVAPDSAAAALEAMRTAGIKLVDGG"
     gene            6714..8036
                     /locus_tag="VFI55_09485"
     CDS             6714..8036
                     /locus_tag="VFI55_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET7074294.1"
                     /translation="MLDKPFGRRSLLRGAGALTAASLTPWAGGCASDDDALTFFFAAN
                     PDERDARMRVVDEFQRRHPDIKVRAVLSGPGVMQQLSTFCAGGKCPDVLMAWELTYAE
                     LAARGVLLDLNTMLARDQAFASQLKSDSIGPLYDIFEFNGGQYGLPEQWSGNYLFYNS
                     RLLAEAGVPAPPKTWDRPWTFTEFLDNATALTKRDGSGRATQWGFVNMWFSYYSAGLF
                     AMNNGVPWSTPLKNPSHFNFDNDAFIEAVQFYADLANKHKVAPDASEVQSMSTPDLFA
                     AGKAAIAMGGHWRYQTFIRAEGLDFDVTSLPVGPRRQGQPACSNIGSTGLAISASSRR
                     KDQAWEFVKFATGPVGQALISESSLFVPVLRSALNSAGFAKAHQRVGNLDVLTQGPAH
                     SEGLPVTPAWEKVVALMDRNFGPVLRGSRSATSLRGLSATVDEVLRTP"
     gene            8033..8992
                     /locus_tag="VFI55_09490"
     CDS             8033..8992
                     /locus_tag="VFI55_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7074295.1"
                     /translation="MTTVDTPAVPGPGTRKDANRSGPSRRRARAGRLFVAPNLAAVAV
                     FMLFPLGFSLYMSFHRWDVFRPPKFVGLKNFEDLFTSDPLFLIAIRNTVIFTLGTVVP
                     TVIVSLIVAGVLNRKVKGIGIFRTIVFLPLAISSVVMAVVWQFVFNTDNGLLNIMLGW
                     IGIGPIPWLVEPRWAMASLCIVSVWRAVPFATVILLAAMQGVPETVYEAAKIDGAGEI
                     RQFVFITMPLIRGAISFVGVISIIHAFQAFDMVYVLNGSNGGPETATYVLGIMLFQHA
                     FSFLEFGYASALAWVMFAILLVLTVVQLRLTGQRSWEASGGLS"
     gene            8979..9821
                     /locus_tag="VFI55_09495"
     CDS             8979..9821
                     /locus_tag="VFI55_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7074296.1"
                     /translation="MVSAERVFTRTVLRAVLVYTALIAIAWCWLFPIAWAISGSLKRE
                     GEVSEPTLLPAHPRWSNYTEVFELMPFWRMFFNTVLYAGCVTAGQVFFCSLAGYAFAR
                     LQFRGRDTLFVLYLGTLMVPLTVTVIPQFILMRAVGWVDTPWAMIVPGLFGSAFGTYL
                     MRQFFRTLPSDLEEAAILDGCSPWQIYWRILLPHARPAVMVLAVLTWVNVWNDFLWPL
                     LMIQRNSLATLTLGLVRLRGEYVARWPIIMATSMLIMLPLVIIYALAQRSFVRGIAVT
                     GLSG"
     gene            9825..10895
                     /gene="ugpC"
                     /locus_tag="VFI55_09500"
     CDS             9825..10895
                     /gene="ugpC"
                     /locus_tag="VFI55_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET7074297.1"
                     /translation="MASVTFEQATRRYPDTDRPALDGLDLVVEDGEFVVLVGPSGCGK
                     TTSLRMVAGLETLDSGRIRIGERDVTNVDPKDRDIAMVFQNYALYPHMTVAQNMGFAL
                     KIARLPKADIRERVLAAAKLLDLQPYLDRKPKDLSGGQRQRVAMGRALVRRPQVFLMD
                     EPLSNLDAKLRVQTRNQIAALQRRLGTTTVYVTHDQVEAMTMGDRVAVLCDGVLQQFA
                     TPRELYRNPANMFVAGFIGSPAMNLFTLPIMDSSVSLGDWPIPLPREIAGAASEVVVG
                     VRPEHFELGGLGVEMEVDVVEELGADAYLYGRITGSGKVIDQPVVARADGRHPPQKGS
                     RVRLHPEPGHLHFFGVDGHRIA"
     gene            10935..11101
                     /locus_tag="VFI55_09505"
                     /pseudo
     CDS             10935..11101
                     /locus_tag="VFI55_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956368.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11140..11415)
                     /locus_tag="VFI55_09510"
     CDS             complement(11140..11415)
                     /locus_tag="VFI55_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007533950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HET7074298.1"
                     /translation="MDLAHWVSSIVAFVRAGYPNGMPTTGYLTLAALARRRPSHDEIS
                     AITSELIMQRSRPITTVDVGVAITRVTNAMPSQDDIARVQRRLEAIG"
     gene            11781..12464
                     /locus_tag="VFI55_09515"
     CDS             11781..12464
                     /locus_tag="VFI55_09515"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET7074299.1"
                     /translation="MIPYSDGIPARRFPVVNTALIAANFAVFLFYELPNGEAAVTRAS
                     FYPCNVDHACHIGVPWGVGWITAMFLHAGWDHILGNMLFLAIFGKNVEDAFGRLGYLL
                     LYFAGGLVATMTQTAMTLLFGSASDAQIPTLGASGAIAAVLGAYLVLYPNSRISTLVG
                     WFPINIPAWVFLGGWFLYQFFESNYALVEPKPGGSGVAFFAHVGGFVFGFLVTRVLVG
                     ADRIAAKQT"
     gene            12520..14610
                     /gene="glgX"
                     /locus_tag="VFI55_09520"
     CDS             12520..14610
                     /gene="glgX"
                     /locus_tag="VFI55_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HET7074300.1"
                     /translation="MTPTIVPGRSGPLGATPDERGTNFAVASGGDEVTLCLFDADGTE
                     TQLVMPDRDADVCHGFVPDVGPGQAYGFRVSGPFDPGRGLRYNPAKLLLDPYARAISG
                     EVRFGPEVLGHTVDNPSEPSSLDSAEHVPRSLVAPIVPPVAVPKPQHALADSIIYELH
                     VRGFTATHPGVPQELRGTYAGLAHEAALQHLVDLGVTTVELLPVHRSVPEQFLVDRGL
                     TNYWGYNTIGFFAPQASYSAAARAGQPGGEIAEFRAMVDALHGAGLEVILDVVFNHTA
                     EGGAGGPTLCFRGLYNPAYYRLDPADASRYVDTTGTGNSLNTDHPTTLRLIMDSLRYW
                     LLEMGVDGFRFDLAPTLGREKGAFDQFSPFFDLVAQDPVISQSKLIAEPWDVGRMDSY
                     EVGRFPPRWSEWNGRFRDTVRDWWRSQDGRLAEFAWRLCGSSDIYGDRAKGRRPSASV
                     NFVTVHDGFTLKDLVSYDHKHNEANGENNRDGTDDNRSWNCGVEGPTDVQAVLDLRAR
                     QERAFLLTLLLSSGVPMLLGGDELGRTQRGNNNAYCQDNDITWFDWSAPDEGLIKFTT
                     DLIALRRRHPVFRRRRFLTGAAAADLRWFTPSGAVMTPQDWGDPEARSVAFFIDGALD
                     PDVDADGTPMVDDDFLVFVNAWWEPLTFSVPGSFALHPWEIVCDSFDPSRTGRAGQRV
                     GIGPRSAVVLRSGR"
     gene            14689..>14867
                     /locus_tag="VFI55_09525"
     CDS             14689..>14867
                     /locus_tag="VFI55_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3097 family protein"
                     /protein_id="HET7074301.1"
                     /translation="MMDRYGTDVLAAGRRKPRSTERPAELGMVVEDAATGYVGAVVRV
                     EYGRVDLEDRHGRTRG"
BASE COUNT         2348 a         4893 c         4951 g         2675 t
ORIGIN      
        1 ctgggcgccg tcgccgttga cgaacggacc cggatcgccg cgggccgctc cgatttgcag
       61 gctcgttccg tcgaagtcga cccagcccgc tttccattcg ccgtaggccg tctcgggtct
      121 cggcggggga ttggcgaggt ccacgagaca ggccagcgcg ccaccggtgt gtttggagtc
      181 cgtcatgcag acgactggcc gaccggcgcc gcctgcggcg ctgaaggcga tgtccttacc
      241 cagctgggtg gtgaggccgt cgcgcgtgac gccgtgatac cgaccgggat cggcggggtg
      301 gcccgcctcg atccacgcga tgacgtctga gatcgccgct cctgctggcg gggcgctcga
      361 aggcacggac ggcttgcccg ccgaggaagg cccagcagag ggtccgctgg tctgcggctg
      421 tcccgagttg ccgttgccgg tgtcggagca tccggcgatc agcagtgata cggcgatgag
      481 cgcggcgatc cgcatcccgc caggctagcg ggccgcgccg cgcagtcggc cgccatacgc
      541 acactacgtg cgtagtgtcg gctaccgtcg ctgttatgca tgagcgcacc gtccgcgccc
      601 gcacaaccac cggcaccgtc gaaggcttca cccgcgacgg agtgaaccgc tggcgatcca
      661 tcccgtacgc gcgcgctccg ctggggccgc tgcgattccg cgcgccgcag ccggcgcagc
      721 cctggtcggg cgtgcggcac tgccacgggt tcaccaactg tgcgccgcag cagcgcatgt
      781 acaccgtgct cggcctatcg ggtttcggag gcaaatacca gcccatgagc gaggactgcc
      841 tcaccctcaa cgtcgtcaca cccgaggccg ccgcggccga accgctgccg gtcatggtct
      901 tcatccacgg cggcggctac atcctgggca gctcggcgac cccggtgtac gacggcgccg
      961 cattggcgcg tcgcggttgc gtgtacgtat cggtcaacta ccggctgggc gcgctgggat
     1021 gcctcgacct gtcgtcgctg tcgagcccgg acatcgccat cgacagcaac ctgtacctgc
     1081 gcgacctggt gctggcgctg cgctgggtcc gcgacaacat cgcgggattc ggcggtgatc
     1141 cggccaacgt caccatcttc ggcgagagcg ccggcgcctg tatcaccgcc acgttgttgg
     1201 cagtccccgc cgccgaaggc ctgttcgccc gggcgatctc ggagagcccg gcgtcgggtc
     1261 tggtgcgctc gaaagacatc gccgccgaat tcgcgacgcg attcgcccag ctgctgggcg
     1321 tgcgcgccca cgatgccgcc caagcgctga tgcgtgcgtc cacggctcaa ctggtggaaa
     1381 cccagcaccg gctgatcgac cagggcacgg aagacaggtt gggcgccttc ccgatcggtc
     1441 ccgtcatcgg cgacgacatg ctgcccctgg atccggtgga ggcgatgcga gacggccggg
     1501 cccatcgggt gccgctcatc gtcggcacca acgccgaaga aggccgcctg ttcacccgct
     1561 tcctcaagat gctgccgacc aaccgatcga tgatcgaaga gttgctggca gacacggaac
     1621 ctgctgcccg cgaacgcatt acggccgcct acccggacta tccggcgccg tcggcatgta
     1681 tccagctcgg cggggacttc gcgttcgcct cggcggcctg gcagatcgcg gaggctcacg
     1741 gcacccacgc gcccgcctat ctctaccggt acgactacgc cccgcggacg ctgcgctggt
     1801 cggggttggg cgcgacgcat ggcaccgaat tgctggcggt cttcgacatc tatcgcacca
     1861 ggttcggcgc actgctgacc gccgcggccg atcggcgtgc cgcgctgcgg gtcagcaatc
     1921 agatacaacg gcgctggcgg tcattcagcc gcaccggttc accgggcgag gactggcccg
     1981 cctacacgcc cgccggccgt gccgtgatgg tcttcgacac caagagccac gtcgagttcg
     2041 atccgcatcc gcagcgccgg atggcatggg acggcttctc cctggcccgt tgacctggca
     2101 cgctgaccct catgcacgcc gacagcgagc aaatcatcga gcgacccggt gacctcacgg
     2161 ccgcttggct gaccgccgcg atcggcgccg gacccgtcgc cgattttgcc gtggagcgca
     2221 tcggcaccgg ccagatgagc gagtgctacc ggatccggct gcattacgcc gacgcccgct
     2281 cggatggacc ggaatcggtg gtgctgaagg tcgcggccac cgacccggtg agccgacaga
     2341 ccgggtcggc cctgggtctc tacgagcgcg aagtccgctt ctacggcgac atcgcaccgc
     2401 gcctggccgg gccgatagct ccctgctacc aggctgcgat cgacacctca acgggcgtgt
     2461 tcgacctgtt gctcggcgat gccgggcctg cggtcgtcgg cgacgaaatc accggggcca
     2521 caatcgaaca ggcccgcctg ggggtcgtcg agctgggacg gctgcaaggc ccgctgctcg
     2581 gggacaccgc gttggccgaa gcgtcgtggc tcaaccgcga gacggcgctc aaccaggcga
     2641 tgatcgcgtc gctctacgcc ggcttcctcg accggtacgg cgaccagatc gccccggagc
     2701 atcgtgtggt gtgcgaacgc ctgatcgccg cgttcgacgg ctacgtggaa gccgaggcga
     2761 ggcccaaccg gatccagggt ctggtgcacg gcgactaccg gctggacaac atgttgttcg
     2821 gcaccgccgg agccgatcgc gcgctgacgg tggtcgattg gcagaccgtt tcccggggtc
     2881 cggcgctgac cgatctgtcc tactttctcg gatgcgcgct gcgaacgcag gatcgtcggg
     2941 accactacga cgcattgctg cgggcctacc acgaggcgct gggacccgag gcgcccatcg
     3001 gcctcgccgg cgtcgccgaa ggtgtgcgcc gccaaagctt tttcggtgtg atgatggcga
     3061 tcgtctcgtc aatgctggtg gagcgcaccg aacgcggtga ccggatgttc atgacgatgc
     3121 tgcaacgaaa ttgcgatcac gcactcgaca ccgaagcgtt gacgacgctg cccgtcgcgg
     3181 tggcgacacc gcccccgcgg ccggcggcgg acgacgaact cgctcacgct cccaccgacg
     3241 agccgctgtg gagtgagagc tggtatgccg acttcatcga tgcggcacag ggattgggcg
     3301 gctggtttcg cgtcggtctg atcgccaacc agcagacggc atgggtgcac gcgctgctgt
     3361 gtggacccgt catgccgacc gtcgccgtcg tggatcccga cgtacggctg ccgccagagc
     3421 cctggcgggt gcccgccgac gccttggagg tgagccactc ggttaccgcc ccgctgcaga
     3481 cctaccgcgt cgacgtgcgt gcgcggggtc gggcttaccc ggacccgtcg gctttgttac
     3541 gcggggaggc gggaaatccc gttgacgtga cgatgaatct ggtgtgggcc accgacggca
     3601 ccccctacaa gtaccggctc acgacgcggt acgagatccc gtgcacggtg tcgggcacgg
     3661 tgaccgtgga cgacaccagc taccgggtcg attcggttcc cggacaacgt gatcattcgt
     3721 ggggtgtgcg ggactggtgg agcatggact ggatatggag tgcgctgcac ctcgacgacg
     3781 gcacccacct gcacggcgtg aacatccgaa ttccgggcgc accgacgttc agcgtcggct
     3841 acgtccagga ggcggacggc ccgcacgtca acgtcaccga gctacagacc gtcgactcac
     3901 gagaagcgtt cggcaccaac gggttaccgc tgcaagcgac cttgattttc caacccggcg
     3961 cgatcgccgc cgatgtcgac gtgcgcggcc acgcgccggt acggctgacc gccgcagacg
     4021 gacgggtgag ccagttcccg cgcgcttggg ccgccatcag cactgcggac ggccgcaccg
     4081 gtgtcggttg ggtggaatgg aaccgcaacc tcgccgagta ggcagacccg cggtccgcga
     4141 cggggcatgc gcacccgttg tggtgatggg tgtctccgga tccggcaaat cgacggtggg
     4201 ggccgcgctc gcacaacggc taggggtgcc tttcgtggat gctgacgtcc tgcacccgcc
     4261 ggtcaacatc gccaagatgg ccgccggtga acccctcaac gacgacgatc ggtacccctg
     4321 gctcgagaag gtcggcgaat ggttggccgg ccaccgcaac ggcggcgtgg caagttgttc
     4381 ggcgctcaaa cgcaaatacc gtgaccagct tcgggcccac tgcccgcggg tcgagttctt
     4441 gtacctcgcc ggctcaccgg aactcatcgc cggccggctg gccgcccggt ccgggcattt
     4501 catgccggcc acactgctgc ggtcgcaatt cgacgcgttg gaacccctcg gcgccgacga
     4561 ggccggtgtg accgtggatg ccgggcaaga cgtcggcgcg atcgtcgatg cctacgtggc
     4621 ggggtcggcg gcgcggcctg gccgggacgc ctgagcgatg tacgtcatcg tcgtcgtgct
     4681 cgtggtggcc gtgcactttg ccttcatcgg ctacgtcgtc gtcggcggtt tcctcgcgct
     4741 gcggtggcgg cgcacgatct ggctgcacgt gccggcggtg atctggggga tcgcgatcgc
     4801 caccaaccgg atcgactgcc ctttgacctg ggcggagcgc tgggctcggc gtaacgccgg
     4861 gatggcgccg ctgccgcccg acggcttcat cgcccactac atcaccggag tgatgtatcc
     4921 cgccggctgg gcgggcggcg tccgcctctc tgtgttggcg ctcgtggcgc tgtcgtgggc
     4981 gatctatgtg cggctgggcg tcgggcgccg ccgacgcgcg atcaccaggg gcgcctgcga
     5041 cgacgagtcc tagccccgtc gccaaccttt tcgcgcgcgg cgtcggccag cttgctgccc
     5101 cgcgcaaccg cggtgtcggc gaactggctg ccgtgcaacc gagcgctttc ggcgagctct
     5161 gcgccgcgca cgcgggccgt gtcagccagc tcttcgcccc tgatgcgggc cttcctggcc
     5221 agtggcgcgc tcttttcggc ggcggtgctg gccagttcgc ggccgcgttc gagagcggct
     5281 tcggcatatg gcgcgcctct ttcggctgcc gtgctggcca gttcgcgtcc gcgttcggcg
     5341 ccgatttgca agccctgcac gatcttttcc ccgagttctc ccaactccgc ttcaaacgtg
     5401 tcgtcggatc ccggcagcgc cgaggacacc ctctcggaga ggcgctcggc cgctcggcgg
     5461 cctcgccatc ccaatgacgg tttgccggcc gtatcggccg acgcgatcat caggccgccc
     5521 aacaggctga ggtcggtcag gaactcccgg cgcttctcgg ctttgatctg cgggtcgctc
     5581 tcactccaga acatgtgcgc accgaggttg gccggtagca ccgtaaaggc aagggccgcc
     5641 gacgcgatac gaggcgcctt gccggtggca agcagcaagc cacctccgat ttggacggcc
     5701 gcgttgagct gtgcgaacgt ctgggcatcg gtggggatgc tactgcccaa cggatccggc
     5761 agggcccgaa gaccatcgac ggtgggcgct gcggcctcgg ccgcagactt gggattgagc
     5821 aacgagtcga ctccctggcc gacgaacgcc gccgacaaca ggggtcgcgc gattctgcgg
     5881 atcaacatga cagctggatt acccggccgg ccggcaagca aaccgtcggg gttgtcgtcg
     5941 cgacggcgat atggtggagc ccgtgcgggc gttgatcatc gtcgacgtgc aaaacgactt
     6001 ctgcgagggt ggctcggtcc cggttaccgg tggcacggcc ttggcgtccg ccatcaacga
     6061 ctacctggcc ggcgagccgg ggtatcacca cgtggtggcg accgaggact accacatcga
     6121 cccggttgat cacttctccg agcggccgga tttcgcgtcg tcctggcccc cgcactgcgt
     6181 tgccggaagc ccgggcgcag actttcagcc ggacctcgac acccgccgaa tcgaggcggt
     6241 cttccgcaag ggtgcttacg ccgcggcgta cagcggcttc gagggcgtcg acgagaacgg
     6301 gacctcgctg ctcgagtggt tgcggcagcg tggggtcgac caggtggacg tcgtcggcat
     6361 cgccaccgac tactgcgtgc ggcgcactgc cgaggacgcg gcgcgggccg gcttgaccac
     6421 cagggtgctg gtggatttga cagctgcggt agcgccggat tcggccgcgg cagcactgga
     6481 agcgatgcga accgccggca tcaagctggt tgacggcggc tgatggcggt gccagtggat
     6541 cgggtgccgt catgctgatc gacggtgtgc ccggcattgt gatcgccgag gatggtcacg
     6601 cgcgggcgct gctgcggtcc tcaccctgcc gccccgcccg cctcagagtc gccacaaccc
     6661 catcgggtac caaggacatc aaccaccgtc ggcaacgcgc caaggggagg acgatgctcg
     6721 ataagccgtt cgggcgccgc agcctgctgc ggggcgccgg tgcgctcacg gcggcgtcgc
     6781 tgacaccgtg ggccggcggc tgcgcgtccg acgacgacgc gttgacgttc ttcttcgcgg
     6841 ccaatccgga cgagcgggat gcgcggatgc gcgtcgtcga cgaattccag cgccgccacc
     6901 ccgacatcaa ggttcgggcg gtgttgtccg ggccgggcgt catgcagcag ctctcgacgt
     6961 tttgcgccgg cggcaagtgt ccggacgtgt tgatggcctg ggagttgacc tacgccgaac
     7021 tggccgctcg cggcgtgttg ctcgacctca acacgatgtt ggcgcgggat caggcatttg
     7081 cctcgcagtt gaaatcggac agcatcggac cgctgtatga catcttcgag ttcaacggcg
     7141 gccagtacgg ccttcccgag caatggtcgg gaaactacct gttctacaac agcagactgc
     7201 tcgccgaggc cggggtgccg gcgccgccca aaacgtggga tcggccgtgg accttcaccg
     7261 aattcctgga caacgccacc gctctcacca aacgggatgg atccggtcgg gccacgcagt
     7321 ggggtttcgt caacatgtgg ttttcctact attcggccgg gctgttcgcg atgaacaacg
     7381 gggtaccgtg gtccaccccg ctgaagaacc ccagccactt caatttcgac aacgacgcct
     7441 tcatcgaggc ggtgcagttc tacgccgacc tggccaacaa gcacaaggtg gcgcccgacg
     7501 cctccgaggt gcagtcgatg tccacgccgg acctgttcgc ggcgggcaag gcggcgatcg
     7561 cgatgggtgg ccactggcgt taccagacgt tcattcgagc cgagggcctc gatttcgatg
     7621 tcacgtcgtt gcccgtgggg ccgcgccggc aggggcagcc cgcctgctcc aatatcggtt
     7681 ccaccggact ggccatctcg gcgagcagtc ggcgcaagga ccaggcgtgg gaattcgtga
     7741 aattcgcgac cggccccgtc gggcaggcat tgataagtga atccagcctg ttcgtgccgg
     7801 tgctgcggtc agcgctcaac tcggctggat tcgccaaagc ccaccagaga gtcggcaatc
     7861 tcgacgtact cacccagggg ccggcgcatt cggaaggact gcccgtcacc ccggcgtggg
     7921 aaaaggtcgt cgccctgatg gaccgcaact tcggacccgt gctgcgcggg tctcggtcgg
     7981 cgacgtcgct gcgggggctg tccgccaccg tcgacgaggt gctgcgcacg ccatgacgac
     8041 cgtcgatacc cccgccgtgc ccgggccggg tacgcgcaaa gacgccaacc gctcgggccc
     8101 ttcgcgacga cgggccaggg ccggtcgcct gttcgtggcg ccgaatctgg ccgccgtcgc
     8161 ggtgttcatg ctgttcccac tcggattctc gctgtacatg agctttcacc ggtgggacgt
     8221 gttccggccg ccaaagttcg tgggcctgaa gaacttcgaa gatctcttca cctctgaccc
     8281 gctgtttctc atcgcgattc gcaacacggt gatcttcacc ctcgggacgg tcgtgccgac
     8341 agtcatcgtc agcctcatcg tggccggcgt gctgaatcgg aaagtcaagg gcatcggaat
     8401 atttcggacc atcgtctttc tgccactggc catctcgtcg gtggtgatgg ccgtcgtctg
     8461 gcagttcgtg ttcaacaccg acaacgggct gctcaacatc atgctcggct ggatcggaat
     8521 cggcccgatc ccatggctgg tcgaacccag atgggccatg gcctcgctgt gcatcgtcag
     8581 cgtgtggaga gccgtgccgt tcgccaccgt catcctgctg gcggcgatgc agggcgtgcc
     8641 ggagacggtg tacgaggcgg cgaaaatcga cggcgcgggc gagatacggc agttcgtgtt
     8701 catcacgatg ccgctgatcc ggggcgcgat ctcgtttgtg ggggttattt cgatcatcca
     8761 cgcgttccag gcatttgaca tggtctacgt tctcaacgga tcgaacggcg gtccggaaac
     8821 cgcaacctac gttctgggca tcatgctgtt ccagcacgcg ttctcgttcc tggaattcgg
     8881 ctacgcttcg gcactggcat gggtgatgtt cgccatcctg ctggtgctga ccgtggtgca
     8941 gttgcggctc acgggccagc ggtcttggga ggcttccggt ggtctcagct gagcgcgtgt
     9001 tcacgcgcac cgtcttgcgt gcggtgctgg tctataccgc gctgattgcc atcgcgtggt
     9061 gctggctgtt cccgattgcc tgggcgattt ccggctcgtt gaagcgggaa ggcgaggtgt
     9121 ccgagccaac gctgctgccc gctcacccgc ggtggtccaa ctacaccgag gtgtttgagc
     9181 tgatgccgtt ctggcggatg ttcttcaaca cggtgctgta tgccggatgc gtaacggccg
     9241 gtcaggtctt cttctgctca ttggctggat atgcgttcgc gcgactgcaa tttcgcggcc
     9301 gcgacacgct gttcgtcctg tacttgggca cgctgatggt gccgttgacc gtgacggtga
     9361 tcccgcagtt catcctgatg cgtgccgtcg gctgggtcga cactccctgg gcgatgatcg
     9421 tgcccggctt gttcggcagc gcattcggca cctacctgat gcgtcagttt ttccgaacgc
     9481 tccccagcga tctcgaagaa gccgcgattc tggacggttg ctcgccgtgg cagatctact
     9541 ggcggatcct gctaccccac gcgcgcccgg ccgtgatggt gcttgcggtg ctcacctggg
     9601 tcaatgtgtg gaacgacttt ctgtggccgc tgctgatgat tcagcgcaac agcctggcga
     9661 cgctgacgct cggcctggtc cggctgcggg gcgaatacgt cgcgcgctgg ccgatcatca
     9721 tggcgacttc gatgctgatc atgctgccac tggtcatcat ctatgccctc gcgcagcggt
     9781 ccttcgtccg cggtatcgca gtgaccgggc tcagcggata aaacatggct tcggtgactt
     9841 tcgaacaggc cacgcgccgc taccccgaca cggatcggcc ggccctggat ggcctcgacc
     9901 tggtggtcga ggacggtgag ttcgtcgttc tggtcgggcc gtcgggatgt ggcaagacga
     9961 cgtcgctgcg gatggtggcg ggcttggaaa cgctggattc cgggcgcatc cggatcggcg
    10021 agcgtgacgt caccaacgtc gatcccaagg atcgcgacat cgcgatggtc ttccagaatt
    10081 acgcgctcta cccgcacatg acggtggcgc agaacatggg ctttgccctc aagatcgcca
    10141 gactccccaa ggctgacatc cgcgagcggg tgctcgccgc cgcgaaatta cttgatctgc
    10201 aaccgtatct ggatcgcaaa cccaaagacc tctccggtgg gcagcggcaa cgcgtggcga
    10261 tggggcgtgc cctggtgcgc cgcccgcagg tgttcctgat ggacgagccg ctgtcgaatc
    10321 tcgacgccaa actgcgcgtg caaacccgca accagatcgc cgcactgcaa cggcgactcg
    10381 gcaccaccac ggtctacgtc actcacgatc aggtcgaagc gatgacgatg ggcgatcgcg
    10441 tcgccgtgtt gtgcgacggc gtgctgcaac aattcgccac gccgcgtgag ctctaccgca
    10501 atcccgccaa catgtttgtc gcggggttca tcggatcccc ggcgatgaac ctgttcacct
    10561 tgccgataat ggattcctcg gtgtcactcg gggattggcc gatcccactg ccgcgtgaga
    10621 tcgccggcgc cgcaagcgag gtcgtcgtcg gggttcggcc cgaacatttc gagctgggcg
    10681 gcctcggtgt cgagatggaa gtcgacgtgg tcgaagagct cggcgcggac gcctacctat
    10741 atggccgaat cacgggctct ggcaaggtga tcgatcagcc cgtcgtcgcg cgcgccgacg
    10801 ggcgccaccc accgcaaaag ggcagccggg tacgcctgca cccagaaccc gggcacctgc
    10861 atttcttcgg cgtcgacggc cacaggattg cttgagcgcc gcaggcgcag ccggtgcgat
    10921 tgggaatcgc aaacatcttc gtgcccgccg acgaagtgag cccgatcgcg ttcgacatca
    10981 ccgccgaaca acgcgaggcc ctcaatcaac ggggtaacag gccggcaaac agtttttgaa
    11041 gacgtggaac cacgcaaact ttctggcggc ctgcggcggt ccggttaagc cgtcgtcttg
    11101 agctgtctga gttattcggt cgggtggctt atcgcgccct catccgatcg cctccaggcg
    11161 gcgttgaacg cgcgcgatgt catcctgtga gggcatcgcg ttggtgacgc gggtgatcgc
    11221 cacgccgaca tcgacggtcg tgatggggcg acttcgttgc atgatgagtt cgcttgtgat
    11281 ggcgctgatc tcgtcatggg acggacgccg gcgcgccaag gccgccagcg tcaggtaacc
    11341 ggtggtcggc atgccgttgg ggtatccggc cctcacaaaa gcgacgatgc tcgataccca
    11401 atgtgccagg tccatgcttc cacctcgcta accgcccgga gctaaacgtc tagggcagtc
    11461 cgtgccacga atgtcaacat agcagcggca aagtcatcag cccggtaaca ccacggttac
    11521 ttacgcacct tttttatgga ttcttttggg ttgcacccgt atccttagcg ccctcccact
    11581 atgaacccgc acaggttctc gtcgcggata atgtcggcgg caacaatgac gatcgaatag
    11641 tctggcgcga cgattgtctc gccaagaccg gtacccgacg gccaaaggga gctcatgaca
    11701 ggcgcgtgtg tcagaacggt ggcgtcgcca ctgaagtcga agtggggacg ccaccgtgac
    11761 ggttccgcgg gactgcccgg atgatcccct actccgacgg tattccggcc cgccggttcc
    11821 cggtagtcaa caccgcgctc atcgctgcca acttcgcggt ctttctcttc tatgaactgc
    11881 cgaacggtga agcggcggtc acccgagcgt cgttctaccc gtgcaacgtc gaccatgcct
    11941 gccacatcgg cgtcccctgg ggagtcggct ggatcacggc gatgttcctg catgccggct
    12001 gggatcacat cctcggcaac atgctgttcc tggcgatctt cggcaaaaac gtcgaggacg
    12061 cattcgggcg gctcggctat ctgctcttgt actttgccgg gggactcgtc gcgacgatga
    12121 cgcagaccgc gatgaccttg ctcttcgggt cggcgtccga tgcgcagata ccgactctgg
    12181 gcgccagcgg cgccatagct gccgtactgg gcgcgtatct ggtgctctat cccaactcgc
    12241 ggatcagcac actggtgggg tggttcccga tcaacatccc ggcgtgggtc ttcctgggcg
    12301 gctggtttct gtaccagttc ttcgaaagca actacgcgct tgtcgaaccc aagccgggcg
    12361 gcagcggagt cgctttcttc gctcacgtcg gcgggttcgt gttcggtttc ctggtaacgc
    12421 gagtgctggt aggcgccgac cgaatcgccg cgaagcagac ctgatgggcc tgagggggag
    12481 ccgccataac gcccttgagg tcatgcgaac ggaggagtga tgacaccgac gatcgtgccc
    12541 gggcggtcgg gcccgctcgg cgccacgccc gatgaacggg gcacgaattt cgcggtggcc
    12601 tccggcggtg acgaggtgac gctctgcctg ttcgatgccg acggcacgga gacgcagctg
    12661 gttatgccgg accgcgacgc cgacgtctgc catggtttcg taccagacgt cgggccgggg
    12721 caggcctacg gattccgcgt cagcggcccg ttcgatcccg ggcgtgggtt gcgttacaac
    12781 cccgcgaagc tgctgctcga cccgtatgca cgtgcgatct caggcgaggt gcggttcggg
    12841 ccggaggtgc tcgggcacac agtggacaac ccttcggagc caagctcttt ggactcggcc
    12901 gaacatgtgc cgcgcagcct ggtggctccg atcgtgccgc ccgtcgccgt gcccaaaccg
    12961 cagcatgccc tcgccgacag catcatctac gagctgcacg ttcgcgggtt taccgcgacc
    13021 catccggggg tgccgcagga gctgcgtggc acgtacgcgg gacttgctca cgaagccgcg
    13081 ctgcagcacc tggtcgacct cggcgtgacg acagtggagc tgctacccgt ccaccgcagc
    13141 gtgcccgaac aattcctcgt cgaccgcggc ttgaccaatt actggggata caacaccatc
    13201 ggattcttcg cgccgcaggc gagttactcc gccgccgcgc gggcgggcca accgggcgga
    13261 gagatcgcgg aattccgcgc gatggtcgat gccctacacg gcgcgggctt ggaagtgatc
    13321 ctcgacgtgg tgttcaacca caccgccgag gggggagccg gcgggccgac cctatgcttc
    13381 cgcgggctgt ataaccccgc ctattaccgg ctcgacccgg ccgacgccag ccgatacgtc
    13441 gacacgacgg ggaccggcaa ctccttgaac accgatcatc cgacgacgct gcggttgatc
    13501 atggactcgc tgcgctactg gttgttggaa atgggtgtcg acggctttcg attcgacctg
    13561 gcgccgaccc tcggccgcga gaagggcgcc ttcgaccagt tctccccatt cttcgatctc
    13621 gtcgcccaag acccggtgat ctcccagtca aagctgatcg ccgagccgtg ggacgtcggc
    13681 cggatggaca gctacgaggt cggccggttt ccgccgcgct ggagcgaatg gaatggccgc
    13741 tttcgtgaca ccgtccgcga ttggtggcgc agtcaagacg ggcgccttgc ggagttcgcg
    13801 tggcggctgt gcggatcatc ggacatctac ggtgatcgtg cgaagggacg ccgcccgagc
    13861 gcatcggtca acttcgtcac cgtgcatgac gggttcacat tgaaagacct tgtctcctac
    13921 gaccacaagc acaacgaggc aaacggcgaa aacaaccgcg acggcaccga cgacaaccgg
    13981 tcatggaact gcggagtgga ggggccgacc gatgtgcagg ccgtgctgga ccttcgggca
    14041 cgacaggaac gcgcgttcct gctgaccctg ttgctgtctt ccggtgttcc gatgctgctg
    14101 ggtggcgacg agctgggccg cacccagcgg ggaaacaaca acgcctactg ccaggacaac
    14161 gacatcacat ggtttgactg gtccgcgccc gacgaggggc tgatcaagtt caccaccgac
    14221 ctcatcgcgc tgcgccgccg tcacccggtg ttccgcaggc gccgctttct cacgggggcc
    14281 gccgccgcgg acttgcgctg gttcacgccg tcaggtgccg tgatgacgcc ccaggactgg
    14341 ggggatccgg aggcgcgcag cgttgcgttc ttcatcgatg gcgccctgga tcccgatgtt
    14401 gacgccgacg gcacgcccat ggtcgacgat gactttctgg tgttcgtcaa cgcctggtgg
    14461 gagccgttga cgttctccgt tcccggcagc tttgcgctgc acccgtggga gattgtgtgt
    14521 gactcgttcg acccctcgcg gaccggtcgg gccggccaac gggttggcat cgggccgcgc
    14581 tccgctgtgg tgctgcgatc cggccgctag caggcgcgcg agcggcggca cggctgcctc
    14641 tggcgcgccg tgacgatgtt tggccgcagg gcttcctaat attgccgcgt gatggatcgc
    14701 tacggaaccg atgttcttgc cgcgggccgg cgtaagcctc gctcgaccga gcggccggcc
    14761 gaacttggca tggtggtcga ggacgcggcg accggctacg tcggtgcggt ggtgcgggtc
    14821 gagtacggcc gcgtcgatct ggaagaccgg cacggccgca cccgcgg
//
LOCUS       DASSGQ010000219         2774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_501956,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000219 DASSGQ010000000
VERSION     DASSGQ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501956"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VFI55_09530"
     CDS             complement(<1..425)
                     /locus_tag="VFI55_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_178027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HET7074302.1"
                     /translation="MSSPRRADGDALRCGDRSAAVTEIRATLATLGLLDNPDEDFITG
                     PHAALELFDGELDEAVRAFQQHRGLLVDGIVGEATYRALKEASYRLGERTLYHQFGAP
                     LYGDDVATLQARLQDLGFYTGLVDGHFGLQTHNALMSYQR"
     gene            complement(556..909)
                     /gene="trxA"
                     /locus_tag="VFI55_09535"
     CDS             complement(556..909)
                     /gene="trxA"
                     /locus_tag="VFI55_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET7074303.1"
                     /translation="MTDAEKAGATIEVSDASFSTDVLSSNKPVLVDFWATWCGPCKMV
                     APVLEEIASERGEQLTVAKLDVDANPETARDFQVVSIPTMILFKDGQPVKRIVGAKGK
                     AALLRELSDEVPNLT"
     gene            complement(906..1916)
                     /gene="trxB"
                     /locus_tag="VFI55_09540"
     CDS             complement(906..1916)
                     /gene="trxB"
                     /locus_tag="VFI55_09540"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET7074304.1"
                     /translation="MTAATSAHDAHESVHDVIVIGSGPAGYTAALYAARAQLSPVVFE
                     GTSFGGALMTTTEVENYPGFRDGITGPELMDEMREQALRFGADLRMEDVESVSLDGPV
                     KSVVTADGQTYWARAVILAMGAAARYLQVPGEQELLGRGVSACATCDGFFFRDQDIAV
                     IGGGDSAMEEATFLTRFARSVTLVHRRDEFRASKIMLDRARNNDKIKFVTNHTVLAVD
                     GETTVTGLRLRNTVTGEETALAVTGVFVAIGHEPRSELVRDVIDVDPDGYVLVQGHTT
                     NTSLDGVFAAGDLVDRTYRQAVTAAGSGCAAAIDAERWLAEHESASENAAGTDTLIGA
                     PR"
     gene            complement(2017..>2774)
                     /locus_tag="VFI55_09545"
     CDS             complement(2017..>2774)
                     /locus_tag="VFI55_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074305.1"
                     /translation="ETADADPPLTVELLADLQAGLLDDDAAARVRKRVREDPAAESVL
                     RALNQVRRDVAALGSDPASAPDVPPEVTSRISAALRSAGAGGPGPSAAHSARPHIRPA
                     KLIAAMAGAGAVLAGICVGTAALINAPEPTPSTPATAEHITVSTPPMTIPLSPAEIVG
                     LLNQTPDYGQIQGQLQGQLSDPPRRASCLTGLGYSASTQVLGARPVEINARPGVLLVL
                     PGETPDSLAVFAVAPNCSAADTGLLASTQIPRP"
BASE COUNT          470 a          908 c          934 g          462 t
ORIGIN      
        1 cgctgatatg acatcaaagc gttgtgggtc tgtaacccga agtggccgtc gaccaggccg
       61 gtgtagaaac cgaggtcctg tagccgcgcc tgcagtgtgg cgacgtcgtc gccgtacagc
      121 ggagcgccga attggtgata cagcgtgcgc tcgccgaggc ggtaggacgc ctccttcaac
      181 gcgcggtagg tggcctcgcc gacgatgccg tccaccagca gaccgcgatg ctgctggaag
      241 gcgcgcaccg cctcgtccag ttcgccatcg aagagttcga gagccgcatg cggcccggta
      301 atgaagtcct catcgggatt gtccagcagg cctagggtgg ccagcgtagc ccggatctcg
      361 gtcacagcgg cgctgcggtc accacagcgc agcgcgtcgc cgtctgcacg gcgcggactc
      421 gacataccaa gggccctccg ggacaacgga caagctagaa gcgggcaaca gccaagcgtt
      481 attgtcgcag atgcttctcg atttcgggaa aacgccaggc tggacgcgcg gcgcggagca
      541 cgaggccgat cgcggtcagg tgaggttggg aacctcgtcg gaaagctcac gcaacagcgc
      601 cgccttgcct ttggcgccga cgatccgctt caccggttgg ccatccttga acaggatcat
      661 ggtggggatc gaaaccacct ggaagtcgcg cgctgtttcg gggttggcgt cgacgtctaa
      721 cttggcgacg gtgagttgct cgccccgctc gctggcgatt tcctcgagaa ccggcgcgac
      781 catcttgcac ggtccgcacc aagtcgccca aaagtcgacc agcacaggct tattgctcga
      841 caacacgtca gtggaaaaag acgcgtcaga cacttctatt gtggcgccag ccttttcggc
      901 atcggtcatc gtggtgctcc aatcaatgtg tcggtacctg cagcgttttc cgacgctgac
      961 tcgtgctcgg ccagccagcg ttcggcgtcg atggccgccg cgcagccgct gcccgctgct
     1021 gtgacggctt gtcggtaggt acggtccacc aagtcgccgg cggcgaaaac accgtccagc
     1081 gaggtgttcg tggtgtgccc ctggacaaga acgtagccgt cggggtcgac atcgatcaca
     1141 tcgcgcacca gctcggaacg cggctcgtga ccgatcgcga cgaaaacgcc ggtcaccgcg
     1201 agggcggttt cttcaccagt taccgtgttg cgcaaccgca atcccgtcac cgtcgtctcg
     1261 ccgtcgaccg caaggacggt gtggttggtg acgaacttga tcttgtcgtt gttacgggcg
     1321 cggtcgagca tgattttcga tgcccggaac tcgtcgcgcc ggtgcaccag cgtcacgctg
     1381 cgggcgaagc gagtcagaaa agtggcctcc tccatcgcgg aatcaccacc gccgatgacg
     1441 gcgatgtcct gatcgcggaa gaagaacccg tcacaggtgg cgcacgcgct caccccccga
     1501 cccagcaact cctgttcgcc gggaacctgc aaataccgcg ccgccgcgcc catggccaag
     1561 atgacggcgc gcgcccaata ggtctgaccg tcggcggtga cgaccgactt gaccggcccg
     1621 tccagggaca ccgattcgac gtcttccatc cgcagatccg cgccgaaccg caatgcctgc
     1681 tcgcgcatct cgtccatcag ctccgggccg gtgatgccgt cacgaaagcc cgggtagttc
     1741 tccacctcgg tggtggtcat caacgcgccg ccgaacgagg tgccctcgaa aaccactggc
     1801 gacagctgcg cgcgcgccgc gtagagcgcc gcggtgtacc cggccgggcc ggatccgatc
     1861 acaatgacgt cgtggacgct ctcgtgagca tcatgggcag aagtggcggc agtcattcga
     1921 gcctttcagg tacggaagct acgtctacga acgccagcgt aggcgcgggt gttcccacac
     1981 ggtcggcaca acacaggcac aacccatggc accacactaa gggcggggga tctgggtgct
     2041 ggccaacaat ccggtatcgg cggcgctgca attcggtgcc accgcaaaga ccgccaggct
     2101 gtcgggcgtc tcgccgggca acaccagcag taccccgggg cgtgcgttga tctcgacggg
     2161 tcgcgcgccc agtacctgtg tggatgccga atagccgagg ccggtgaggc aggacgcgcg
     2221 gcgtggcggg tcgctcagct ggccttggag ctgaccttgg atctgaccat agtcgggtgt
     2281 ctggttgagc aggccgacga tctctgccgg cgagagcgga atcgtcatcg gcggcgtaga
     2341 caccgtgatg tgctcggcgg tggccggggt gctgggcgtg ggctccggcg cgttgatcag
     2401 cgccgcggtg cccacgcaga tccctgcgag caccgcgccc gcgccggcca tggcggcgat
     2461 caacttcgcg ggccgtatgt gtggccgggc cgagtgcgcg gcgctgggcc cgggtcctcc
     2521 cgcgcccgcc gaccgcagcg ccgccgagat tcgggaggtg acctcgggtg ggacgtcggg
     2581 cgccgacgcc gggtcgctcc cgagcgcggc gacgtcgcgg cgcacctggt tcagggcgcg
     2641 caggacgctc tcggccgccg ggtcttcgcg gacccgcttc cgtacccggg cggcggcatc
     2701 gtcgtcgagt agaccggcct gcaggtcggc gaggagttcg accgtcaggg gcgggtcggc
     2761 gtccgctgtc tctg
//
LOCUS       DASSGQ010000220        10123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_350359,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000220 DASSGQ010000000
VERSION     DASSGQ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10123
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350359"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFI55_09550"
     CDS             <1..193
                     /locus_tag="VFI55_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HET7074306.1"
                     /translation="QQANVPLGIIPLLQVDMWEHAFYLQYKNVKADYVKAFWNVVNWA
                     DVQDRYAAATAKTKGLIFG"
     gene            385..918
                     /locus_tag="VFI55_09555"
     CDS             385..918
                     /locus_tag="VFI55_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HET7074307.1"
                     /translation="METGSDRPIGVSPFHSRGALKGFVISGRWPDSTKEWAQLLMVAV
                     RVASLPGLLSTTTVFGAREELPDEPEPGTVGLVLAEGTVFGESAIRPGYFANHQPPAL
                     LMLHPPSETTPSLPECTGAASGCVLLPGLPYLGLEHRAAWVEAEADGTITSMVSRVGV
                     DPISHPDTAILAMLLAA"
     gene            1173..1868
                     /locus_tag="VFI55_09560"
     CDS             1173..1868
                     /locus_tag="VFI55_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HET7074308.1"
                     /translation="MLAATLLSLGVVFLAELGDRSQLITMTYALRYRWWVVLTGVALA
                     AFVAHGVSVTVGHFLGAALPARPLAFASAIAFLIFAVWAWREGGSGDQSVSTKVEPRF
                     ALFTVMSSFALAEMSDKTSLATVTLASNHDWRGVWIGSTVGMVLADGLAIGVGMLLHK
                     RLPKRLLHVLASLLFSIFGLWMLFDSALGQRRVAIAVTVAVVVAAATVAVAQALRRRR
                     KEASTAGRSPDIA"
     gene            1991..2122
                     /locus_tag="VFI55_09565"
     CDS             1991..2122
                     /locus_tag="VFI55_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074309.1"
                     /translation="MLLSTRLSCLLIAVQLPSILGKIASPETTLATRHAAPDPGFCG"
     gene            2324..2722
                     /locus_tag="VFI55_09570"
     CDS             2324..2722
                     /locus_tag="VFI55_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET7074310.1"
                     /translation="MSTTFAARLNRLFDTVYPPGRGPHTSAEVIAALKAEGITMSAPY
                     LSQLRSGNRTNPSSATMAALANFFRIKPAYFTDDEYYEKLDKELSWLSTMRDDGVRRI
                     ALGAAELSAESRKDVAERVNELRRKEHLAV"
     gene            2857..3513
                     /locus_tag="VFI55_09575"
     CDS             2857..3513
                     /locus_tag="VFI55_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6474 family protein"
                     /protein_id="HET7074311.1"
                     /translation="MGLFRKRKSRSTRRAEARAIKARARLEAKLAAKNEARRIKGERR
                     AAEKSLKAHVRSQRDSDRAALKVAETELKAAREGKLLSPTRIRRALTVSRLLAPILTP
                     LIYRGAMTARAMIDRRRADELGIPLAQIGQFSGHGAQLSARISGAEKSLRMVQDKKPK
                     DPETRQFVSAIVERLTDLSAAVTAAENMPAPQRRAAHAAISSQLDGIEADLMARLGLS
                     "
     gene            3522..4202
                     /locus_tag="VFI55_09580"
     CDS             3522..4202
                     /locus_tag="VFI55_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009955499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcnI family protein"
                     /protein_id="HET7074312.1"
                     /translation="MSTHDRRFSRVLIPLVGAAGLYAGAAMSTPAAWAHVHASSDDPV
                     RGSTAIVTFEVPNESNTGSATTALTVTLPDVTSASTESMAGWTAKLDRDAASGTVHSV
                     TWTAGPNGGIGADQFALFRISLQLPDADTVSFPATQTYADGTVVKWDQPPLPGGGEPE
                     HPAPMLTLATGSATPHEHHPPSGAPADHAAAPQQPKSPDNIARLLGGAALVVAAVGIC
                     LGLIRRRT"
     gene            4199..4708
                     /locus_tag="VFI55_09585"
     CDS             4199..4708
                     /locus_tag="VFI55_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HET7074313.1"
                     /translation="MSRPAVIGWAGLLLAATLLAALLTAPAAFAHAWRLSTDPADNAA
                     LTTGPERASATFNERLQTTFAAMTVVGPDGNVWSSGEPTVQGAVVGIGLRPLGPAGTY
                     TVNYRVTSADGHVVSGSWSFRLTVPGTGTPGSPARTDAGDELPVWPFVVLVVALVAAG
                     GVWAARRRQ"
     gene            4954..5412
                     /locus_tag="VFI55_09590"
     CDS             4954..5412
                     /locus_tag="VFI55_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-structuring protein H-NS"
                     /protein_id="HET7074314.1"
                     /translation="MSGPPDPAPPGPPDPAPPGPPEKAPPAKAVKKTAKAPAKKAPAK
                     KAPAKKAPAKKAAAKKAPAKSPEPPPQNPNERPAGQPLGIAQRIETNGQLAAAAKDVA
                     AQAKSTVEGANNPVLPAVPAPSPGQSPVPLIISLTLSLLAILLIRQLRRR"
     gene            5437..5919
                     /gene="rraA"
                     /locus_tag="VFI55_09595"
     CDS             5437..5919
                     /gene="rraA"
                     /locus_tag="VFI55_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HET7074315.1"
                     /translation="MTVSFQPTADLVDSIGPDVRSCDLQFRQFGGRSQFAGPISTVRC
                     FHDNALLKSVLSGPGAGGVLVIDGAGSLHTALVGDLIAELARSNGWAGLIVNGAVRDA
                     AALRGIDIGIKALGTNPRKSTKAGAGERDVEITLGGVTFVPGEIAYSDDDGIVVASAD
                     "
     gene            complement(5916..7385)
                     /locus_tag="VFI55_09600"
     CDS             complement(5916..7385)
                     /locus_tag="VFI55_09600"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7074316.1"
                     /translation="MTEHLDVVIVGAGISGVSAAWHLQDRCPTKSYAILEKRAAMGGT
                     WDLFRYPGIRSDSDMYTLGFRFRPWTERQAIADGKPILDYVKGTAAMYGIDKHIRLNQ
                     KVVSADWSSADNRWTLQIESDGTAKTITCTFLFLCSGYYNYEQGYAPAFAGAEDFTGP
                     IIHPQHWPEDLDYAGKNVVVIGSGATAVTLIPALADSGVGHVTMLQRSPTYIVSQPKR
                     DKLAEWLNRWLPEKQAYTAVRWKNVLRQAALYRACQKFPRRMRNLLLGYVQRQLPEGY
                     DVQKHFGPHYNPWDQRLCLVPSGDLFRAIRHGQAEVVTDTIERFTPTGIRLNSGQELP
                     ADIIITATGLNLQLFGGASVSVDGQPADLTKTMAYKGMMLSGLPNMVYTVGYTNASWT
                     LKADLVSEFACRLLNYMDAEGFDTVVVEHPGPDVGERPFMEFTPGYVLRALDELPKQG
                     SRTPWRLNQNYLRDIRLIRRGKIADEGLRFAKKPVAVPV"
     gene            7463..8104
                     /locus_tag="VFI55_09605"
     CDS             7463..8104
                     /locus_tag="VFI55_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7074317.1"
                     /translation="MTTAGQTRAVRGRRAARPSGDDREQAILATAERLLEERAFADIS
                     VDDLAKGAGLSRPTFYFYFRSKEAVLLSLLEPVITRADSEFDGAVQRLPSDPRRVWRN
                     GIKAFFTEFASRPALARAATEALATSAELRAVWSEFMQKWIDQTAALITAERERGAAP
                     QTIPAVDLATSLNQMNERTMMAALAAETPAVDADRVVDTLTHIWVSSIYGESG"
     gene            8140..9354
                     /gene="dinB"
                     /locus_tag="VFI55_09610"
     CDS             8140..9354
                     /gene="dinB"
                     /locus_tag="VFI55_09610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET7074318.1"
                     /translation="MFVRCDASILHADLDSFYASVEQRDDPTLRGRPVIVGGGVVLAA
                     SYEAKAYGVRTAMGGAQARRLCPHAVVVPPRMNAYSRASDAVFEVFRDCTPIVEAVSV
                     DEAFLDVAGLRRVSGAPVEIAERLRADVRDRVGLPITVGIARTKFLAKVASQEAKPDG
                     LLVVPPDQELAFLRPLPVRRLWGVGAVTAEKLHAHGIVTVADVAELSASTLASLAGAA
                     MGRQLYALSRNIDRRRVTTGVRRRSVGAQRALGRAGNRMSAAEVDAVVVNLIDRITAR
                     MRAAGRTGRTVVLRLRFDDFGRATRSRTLPWATSSTQPILAAARLLVATAAPLIAQRG
                     LTLVGFAVSGIDRSGAQQLMLPFGEAGARLASDDVDAAVDRVRRRYGKSALTRAVLVG
                     RDPGFEMPHLPD"
     gene            complement(9668..>10123)
                     /locus_tag="VFI55_09615"
     CDS             complement(9668..>10123)
                     /locus_tag="VFI55_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074319.1"
                     /translation="TERPVYRFNMLDTTTTLNGAEAVKEVYREWTRTAQCIFYTGGDE
                     KLAVSDVMIVSTSTLYQQFPGTILAATGATIDPDTTYLVKMAAHMIWPYDDRGRLIGE
                     DVWEYDDSVREIIPLDSADVLTAEQSGKLLAPLIKPLPTNNPFTAPAVV"
BASE COUNT         1662 a         3406 c         3367 g         1688 t
ORIGIN      
        1 ccagcaggcc aacgtcccgc tcggcatcat cccgctgctt caggtcgaca tgtgggagca
       61 cgccttctac ctgcagtaca agaatgtcaa ggcggactac gtcaaggcgt tctggaacgt
      121 tgtcaactgg gctgacgtac aggaccgcta cgcggccgcc accgcgaaaa ccaagggcct
      181 gattttcggc tgagaccacc tcgaaaaggg ccggggcgtc acgcggatcg cgtggcgccc
      241 ctgcctttct ccgggccttt gtcaggccac ccgatcaagg taagaactat tagccgtgtg
      301 gagttgcaac gcgcctaaat cgcaggcatc cttagttatt cggcagcgtg gtcattcgga
      361 cgaaggcgtc gcgcggaggt cgagatggag acagggtcag accgtcccat aggggtttcc
      421 cccttccatt cccgtggtgc cctgaaaggg tttgtgatct ccgggcgttg gcccgattcc
      481 acgaaagaat gggcccaatt attgatggtc gccgtccgcg tcgcgtcatt gcccggattg
      541 ctctccacca caacggtgtt cggcgcccgc gaggagttgc ccgacgaacc cgagcccggc
      601 accgtcggcc tggtgctggc cgaaggcacg gtgttcggcg aatcagccat ccggccagga
      661 tatttcgcca accatcagcc tcccgcgttg ctgatgctgc atccgccctc ggaaaccaca
      721 ccttcgctgc cggagtgcac cggggcggcc tccggctgtg tgctgctgcc gggactgccc
      781 tacctgggat tggagcaccg agcggcctgg gtggaagccg aagccgacgg caccatcacg
      841 tccatggtga gtcgggtggg agtcgatccg atcagccatc ccgacacggc cattctggcg
      901 atgctgcttg ccgcataagg caattcaaac gaattagttc gcccaccgaa tcgagaaata
      961 cgggcgccct aacttgatcc gtgcctggcg cgcgccgaat gcgcccgcta gcctgaaaac
     1021 cgttagcgaa ggggagtagc cccgaatcgt cgtgtcgaca tactggcgca ttgcccggcc
     1081 ggcgaaccgg tccggccgga ccggtgggcg agaccttcga ccgaagttcg atgacgtaac
     1141 ggtcgtcgac gacgtgtcga aaggtcctct tcgtgctcgc agccacgttg ctcagtctcg
     1201 gagtggtctt ccttgccgaa ctcggcgaca gatcgcagct catcacgatg acctacgcgc
     1261 tgcgctaccg atggtgggtg gtcttgaccg gggtggcgct cgccgcattc gtggcgcacg
     1321 gcgtctcggt gaccgtcggc cacttcctgg gcgccgcgct gccggcgcgc ccactggcct
     1381 tcgcgtcggc gatcgcattc ctgattttcg cggtgtgggc ctggcgtgag ggaggctccg
     1441 gtgaccaaag tgtgtcgacc aaggtggaac cgcggtttgc actgttcacc gtgatgtcgt
     1501 cgttcgcgct tgccgaaatg agcgacaaga cgtctttggc gacggtgacg ttggccagta
     1561 atcacgactg gcggggcgtg tggatcggct cgaccgtggg catggtcttg gccgacggtc
     1621 tggcgatcgg tgtgggaatg ctgctgcaca aacgccttcc taagcggctg ctgcacgtct
     1681 tggccagcct gctgttctcg atattcggct tgtggatgct gtttgacagc gcactcggcc
     1741 agcgccgggt ggcgatcgcc gtgaccgtcg cggtggtggt ggctgccgca actgtggcgg
     1801 tggcgcaagc tctgcgacga cgccggaaag aggcttcgac cgcggggagg tcacctgaca
     1861 tcgcctaggg tcccgacgag acacactcgc catgccctcg ccatgccccg cagtcgcccc
     1921 agaggtatcg gcgaaggcca caggtcgtac tgcgcgcccg tttacgctcg cgtcaccgtc
     1981 cggacagcaa atgctgctgt cgacacgcct aagttgcctg ctgatcgcgg tgcaactgcc
     2041 cagcatcctc ggaaagatcg cttcgccgga gacgacgctg gcaacccgcc acgctgcccc
     2101 cgatcccgga ttctgcggat aacctgtgag tttcggcaat ttagtagaca cctcagcgaa
     2161 ttagttagac gctcgtcgtg tggggatttg gcatccgtca gcaacgcccg tgtcggagcc
     2221 agcggggttt gcacctggtt ggggacccgg cccctcgcta cgatgatcag caaatacacc
     2281 tcggcaatag ttaacttcta cagccaagtg gaggtcaata tcgatgagca cgactttcgc
     2341 tgcccgcctg aaccgtttgt tcgacacggt gtatccaccc gggcgcgggc cacacacttc
     2401 cgcggaggtg atcgcggcgc tcaaggcgga gggcatcacg atgtcggctc cctacctgtc
     2461 ccagctgcgt tcgggcaacc gcaccaaccc gtcgtcggcc accatggcgg cgctggcaaa
     2521 tttcttccgc atcaaaccgg cgtacttcac tgacgacgag tactacgaga agctcgacaa
     2581 ggagttgtcg tggctctcca cgatgcgcga tgacggtgtg cgccgcatcg cgctgggcgc
     2641 cgccgagttg tctgccgaat cacgcaagga cgtcgccgag cgggtcaacg agctgcgccg
     2701 caaggagcat ctcgcggtct aggtccgcca ccagccgcat tacccgacct caacggttgc
     2761 ccgctggccc attgcgatta gggttggctc acgatcgcgc acgcgcacgc gcgacagaca
     2821 acgatagcca gcgagattac cgggaggcgg ccgggaatgg gcctgttccg caagcgaaag
     2881 agtcggtcga cgcgtcgcgc cgaagcccgt gcgatcaagg cccgcgccag gctggaggcc
     2941 aagctggcgg ccaagaacga ggctcgccgt atcaagggtg aacgacgcgc cgccgaaaag
     3001 tccctcaagg cacacgtcag gtctcaacgg gacagcgatc gtgcggcgtt gaaagtcgcc
     3061 gagactgagc tcaaggcggc gcgggagggc aagttgttgt caccgactcg gatccggcgg
     3121 gcgctgacgg tgtcccggtt gctcgcgccg atcctcacgc cgctgatcta ccgcggcgcc
     3181 atgaccgccc gcgcaatgat cgaccggcgt cgcgcggacg agctcgggat tccgttggca
     3241 cagatcggcc agttttccgg gcatggcgcc cagctgtcgg cccggatctc cggggcggaa
     3301 aagtcgttgc ggatggtgca agacaagaag cccaaggacc ccgagaccag acaattcgtg
     3361 tcggccatcg tcgaacggct caccgatctg tcggccgccg tgaccgccgc ggagaacatg
     3421 ccggccccac aacgccgggc cgcccatgcc gccatctcgt cgcaactcga cggtatcgag
     3481 gcggacctga tggcccgcct cggattatcc tgaccgctga catgtcaacc cacgacaggc
     3541 ggttttcgcg cgtcctgatc ccgctcgttg gggccgccgg actgtacgcc ggcgcagcga
     3601 tgagcacgcc ggcggcatgg gcgcacgtcc atgccagcag cgacgacccg gtacgcggca
     3661 gcaccgcgat cgtcaccttc gaggtgccca acgaatccaa cacaggctcg gcaaccaccg
     3721 cgctgacggt cacgctcccc gatgtgacat ccgccagcac cgaatccatg gcggggtgga
     3781 cggccaagct cgaccgcgac gcggcttccg gcaccgtgca ctcggtgacc tggacggccg
     3841 ggcccaacgg cgggatcggc gcggatcagt tcgcgctgtt ccggatttcg ctgcagctgc
     3901 cggacgccga cacggtcagt ttcccggcca cccagaccta tgccgatggg acggtggtga
     3961 agtgggacca gccgccgctg cccggcggcg gtgagcccga acatccggcc cccatgctga
     4021 cccttgccac cgggtcggcg acaccgcatg agcaccatcc gccgtccggt gcgcccgccg
     4081 atcacgctgc ggcgccgcag cagccgaaat cgccggacaa catcgcccgc ctgttgggtg
     4141 gggcggcctt ggtggtagcg gctgtgggga tatgtcttgg gctgatccgc cgacggacat
     4201 gagtcgcccg gcggttatcg gctgggccgg cctgctgctg gccgccacgc tgttggccgc
     4261 gttgctcacc gcgccggcgg cgttcgcgca tgcctggcgg ttatccaccg atcccgcgga
     4321 caatgcggcc ctcaccaccg gtcccgagcg ggcgagcgcg accttcaacg agcgattgca
     4381 gaccacgttc gcggcgatga ccgtcgtcgg cccggacggc aacgtgtggt cctccgggga
     4441 gccgacggtg cagggcgcgg tcgtcggcat cggcctgcgg ccgcttggac ccgccgggac
     4501 ctacacggtg aactaccgcg tgacctcggc agacggtcac gtggtgtccg ggtcctggtc
     4561 gtttcggctg acggtgccgg gtaccggaac gcccggatcg cccgctcgca ccgatgccgg
     4621 cgacgaattg ccggtgtggc cgttcgtggt gctggtggtc gcgctcgtcg ccgcgggcgg
     4681 cgtgtgggcg gcccggcgca ggcagtgacc ggccctggtc aacgagccgc cgccgccctt
     4741 cctggcatga tgggacccga gcaactcggt gcgctagaaa cgagcaagag gaagttgcaa
     4801 aggagcggcc gcatggcaga cccgcaggat caaccgaata gcgaagccga cggcgcgccg
     4861 accccgcctg gtccacccgg cccgcctgac cccgcgccgc ctggcccacc cggcccgcct
     4921 gaccccgcgc cgcctggccc gtctgacccc gccctgtctg gcccgcctga ccccgcgccg
     4981 cctggcccgc cggatcccgc gccgcctggc ccgccggaaa aggcgcctcc cgccaaggcc
     5041 gtcaagaaga ccgcgaaagc gcccgccaag aaagcacctg cgaaaaaggc gcccgccaag
     5101 aaggcgccgg cgaaaaaagc cgccgccaag aaggccccgg ccaaaagccc tgaaccccca
     5161 cctcagaacc ccaacgagcg gccggccgga caacccctcg ggattgcgca gcggatcgaa
     5221 accaacggcc agcttgccgc tgccgccaaa gacgttgccg cacaggccaa atcgacggtg
     5281 gagggcgcaa acaaccccgt cttgcccgcc gttcctgcgc cgtcaccggg ccagtcgccg
     5341 gtgccgctga tcatttcgct gaccctcagc ctgttggcga tcctgctgat ccggcagttg
     5401 cggcgccgct gaagcggcct ggctagcatg tctgaagtga ccgtgtcatt ccagccgacc
     5461 gccgacctcg tcgacagcat cgggccagac gtacgcagct gcgacctgca gttccgccag
     5521 ttcggcggcc gttcccaatt cgccggcccg atcagcaccg tgcgctgctt tcacgacaat
     5581 gcgctgctga aatcggtgct gtcggggccc ggcgcgggcg gagtgctggt gatcgatggc
     5641 gccggctccc tgcacaccgc gctggtcggt gacctcatcg ccgaattggc ccgttccaac
     5701 ggctgggcgg gtctgatcgt caacggcgcg gtgcgcgacg ccgcggcgtt gcgcggcatc
     5761 gacatcggaa tcaaggcgct gggtaccaac ccgcgcaaga gcaccaaggc cggggccgga
     5821 gagcgcgacg tcgagatcac cctgggcgga gtgacgttcg tccccggcga gatcgcctac
     5881 agcgacgacg acggcatcgt cgtcgcttca gctgactaaa ccggtactgc cacaggtttt
     5941 ttggcgaatc gcaggccctc gtcggcgatc ttgccgcgcc ggatcagccg gatgtcgcgc
     6001 aggtagttct ggttcagccg ccacggggtg cgcgaacctt gcttgggcaa ctcgtcgagc
     6061 gcgcgcagca cgtagccggg cgtgaattcc atgaacggac gctccccgac gtccgggccc
     6121 gggtgctcga ccaccaccgt gtcaaaacct tcggcgtcca tgtagttcag tagacggcag
     6181 gcgaactccg acaccaggtc ggccttcagc gtccacgacg cgttggtgta gcccaccgtg
     6241 tagaccatgt tgggcagacc ggacagcatc atgcccttgt aggccatggt cttggtgagg
     6301 tccgccggtt gcccgtcgac gctcacagac gcgccgccga acagctgcag gttcaaaccc
     6361 gttgcggtga tgatgatgtc ggccggcagc tcctggccgg agttgagccg gataccggtc
     6421 ggggtgaacc gctcgatggt gtcggtgacc acctcggcct gcccgtgccg gatcgcgcgg
     6481 aacaggtcgc cactgggcac caggcacagc cgctgatccc acgggttgta gtgcgggcca
     6541 aagtgcttct gcacgtcgta gccctcggga agctggcgct gcacgtagcc cagcaggagg
     6601 tttcgcatcc gccgcgggaa cttctgacag gcccggtaca gcgccgcctg ccgcagcacg
     6661 ttcttccagc gaaccgcggt gtacgcctgc ttctcgggca gccagcggtt gagccactcg
     6721 gccagcttgt cccgcttggg ctgagagacg atgtaggtcg gtgaccgctg cagcatcgtc
     6781 acgtgcccga cgcccgaatc cgcgagcgcc ggaatcagag tgaccgcggt cgcgccactg
     6841 ccgatcacga cgacgttctt gcccgcgtag tcgaggtcct cgggccagtg ctgcggatgg
     6901 atgatcgggc ccgtgaagtc ctccgcgccc gcgaacgccg gcgcgtagcc ctgctcgtag
     6961 ttgtagtagc cactgcacaa gaagaggaag gtgcaggtga tggttttcgc ggtgccgtcg
     7021 ctctcgatct gcagggtcca gcggttgtcc gcgctggacc aatcggcgct gacgaccttc
     7081 tgattgagcc ggatgtgctt gtcgatgccg tacatcgccg cggtgccctt gacgtagtcg
     7141 aggatcggct tgccgtcggc gatggcctgg cgctcggtcc acggacggaa ccggaagccg
     7201 agcgtgtaca tgtcggagtc ggatcggatc ccggggtagc ggaagagatc ccaggtgccc
     7261 cccatagcgg cgcgtttctc caggatcgcg tagctcttgg tcgggcagcg atcctgcagg
     7321 tgccacgccg cgctcacccc ggagatgccg gcgcccacga tgacgacatc aagatgctca
     7381 gtcatgcggc ccacgttatc aacgcagtgt cgaactcgtc aacggtgtgt cgaatctttc
     7441 aactccgtgt aaagtaaccg ccgtgactac cgccggccag acccgtgccg tgcgcggccg
     7501 ccgcgccgcg cgcccttctg gcgacgaccg cgaacaggca atccttgcga ccgccgaacg
     7561 actgctcgaa gagcgggcgt tcgccgacat ctcggtcgac gacctggcca agggcgcggg
     7621 cctgtcgcgg ccgacgttct acttttattt caggtccaag gaggcggtgc tgctctcgct
     7681 gctcgagccg gtgataacgc gtgccgactc cgaattcgac ggcgcggtgc agcgcctgcc
     7741 cagcgatccg cgccgcgtgt ggcgcaacgg catcaaggcg ttcttcaccg aattcgcctc
     7801 gcgcccggcg ctggcccgcg ccgccaccga ggcgctggcc accagcgcgg agttgcgggc
     7861 cgtgtggtcg gaattcatgc agaagtggat cgaccagacc gcggcgctga tcaccgcgga
     7921 gcgggaacgc ggcgccgcac cgcagaccat tcccgccgtg gacctcgcga cgtcgctgaa
     7981 ccagatgaac gaacgcacca tgatggccgc gctggccgcc gagacgccgg cggtggacgc
     8041 ggaccgtgtc gtcgacaccc tcacccacat ctgggtcagc agcatctacg gcgaatccgg
     8101 ctaacttttt cgcggcgtgt cacccccgca tgcgaacatc tgttcgtgcg gtgtgatgcg
     8161 tcgatcctgc acgcggacct ggattcgttc tacgcgtccg tcgaacagcg cgacgacccg
     8221 acgctgcgcg gccgcccggt gatcgtcggc ggcggggtcg tgctggcggc cagctacgaa
     8281 gccaaggcct acggcgtgcg cacggcgatg ggcggggcgc aggcgcggcg cctgtgcccg
     8341 cacgccgtgg tggtgccgcc ccgaatgaac gcctacagcc gcgccagcga cgccgtgttc
     8401 gaggtgttcc gtgattgcac accgatcgtg gaggcggtgt cggtggacga ggcattcctc
     8461 gacgtcgccg ggctgcgccg ggtctcgggt gcgccggtgg agatagcgga gcggctgcgc
     8521 gccgacgtgc gcgaccgtgt cggcctgccc atcaccgtcg gcattgcccg gacgaaattc
     8581 ctcgccaagg tcgccagcca ggaggccaag cccgacggac tcctggtggt accgccggat
     8641 caggagctgg cattcctgcg cccgttgccg gtacgccgcc tgtggggcgt gggtgcggtg
     8701 accgccgaga aactgcacgc gcacggcatc gtgaccgtcg ccgacgtcgc cgagctcagc
     8761 gcgtcgacgc tggcgtcgct ggcgggcgcc gcgatgggac gccagctgta tgcgctgtcc
     8821 cgcaacatcg accgccgccg ggtcaccacg ggtgtgcgcc gccggtcggt gggcgcccag
     8881 cgggccctgg gccgcgcggg caatcgaatg tcggccgccg aggtcgacgc ggtggtggtc
     8941 aacctgattg accgcatcac cgcgcggatg cgcgccgccg ggcgcaccgg tcgcacggtg
     9001 gtgctgcgcc tgcggttcga cgacttcggc cgggcgacgc gatctcgcac gctgccgtgg
     9061 gccacgtcgt cgacgcagcc catcctggcc gcggcccggc tgttggtcgc aacggcggcg
     9121 ccgctgatcg cgcagcgcgg gctgaccctg gtcggcttcg cggtgtcggg catcgaccgc
     9181 agcggcgccc agcagctgat gctgccgttc ggcgaggctg gagcccggct cgcctcggat
     9241 gacgtcgacg cggcggtcga tcgggtgcgc cgtcgctacg gcaaatccgc gctcacccgc
     9301 gcggtgctgg tcggccgtga cccgggcttc gagatgccgc acttacccga ctgacgggat
     9361 cgcgcggatc aaccggccca cgttgcgtcc gcagcactga cgccagggcc catgccgcgc
     9421 gaattgttgc ctcgcaggca ctgccgcggc agttggggtg ttccgtgaac ggggatcgtc
     9481 caacgcgatg gagctcgatg cgcgcccgga cctctccaaa ctcgattcgc ccaacatgcg
     9541 gcgttaggcg atcgcacatg gcgtgtccac gttcgagtac gccgcttgta cgacggcgca
     9601 tatggtcgac tatgcggccg ctcgcgcact ccgcgcaaga aacgccgccg cgatgcgcgc
     9661 gcgtgggcta gaccacagcc ggcgcagtga acgggttgtt ggtcggaagc ggcttgatga
     9721 gtggcgcgag caacttgccg gattgctcgg cggtgaggac atcggccgaa tcaaggggga
     9781 tgatctcgcg gaccgagtcg tcgtattccc aaacatcctc accgatcaag cgtccgcggt
     9841 cgtcgtatgg ccagatcatg tgtgcggcca tctttaccaa gtaggtagtg tcggggtcga
     9901 tggttgcacc ggtggcagca aggatggtcc ccggaaactg ctggtacagc gtagacgtcg
     9961 agacgatcat gacgtcgctc accgcaagct tctcgtcgcc gcctgtgtag aagatgcact
    10021 gagccgtacg ggtccactcg cgatagacct ctttgactgc ctcggccccg ttaagcgtcg
    10081 tggtcgtatc gagcatgttg aagcgataga caggcctctc ggt
//
LOCUS       DASSGQ010000221         7201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_227534,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000221 DASSGQ010000000
VERSION     DASSGQ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7201
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227534"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VFI55_09620"
     CDS             <1..411
                     /locus_tag="VFI55_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HET7074320.1"
                     /translation="LLPAAADEVSASIAHLFSAHAQDYQVVAGQAAAFHQQFMQNLTA
                     GAGAYASAETANAAVLRPLNAIAGSVASAAAPINPLTPLTEWYDTVMSQLQNAIGGLL
                     FFLLSPILTPIIDAIAKALVDAFIEALLGQSSTT"
     gene            517..1059
                     /locus_tag="VFI55_09625"
     CDS             517..1059
                     /locus_tag="VFI55_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074321.1"
                     /translation="MARTGPPDEFHHPQTQYPNYGMGGYPPPAVPPGPPPEPPPERQG
                     PPDYLDPLDQEVEPTPWYRKPAGLISWLVAVLILIGLIIYGIIELIQGEGGGYTPGTS
                     TTTTPATVTSTTPSTTPPTSSATTTTPTAPPTSSAVEPPTRQPTQQQPTHRHHLPPLP
                     SVITIPEVPTVITLPPGLTD"
     gene            1370..3007
                     /locus_tag="VFI55_09630"
     CDS             1370..3007
                     /locus_tag="VFI55_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET7074322.1"
                     /translation="MSESLGLSIGVANLVAARVGSAPVTRSSVVTLFEHRPTEAGLPE
                     ENPNLTEPGLVLRGFVERVGDRAPLVAADGTKYAGAALTVEAIEAMARTVGYGTPVTI
                     AIPAYWSEAQSSALREEFFAQPDLARAGVPPVLVSDATAALAALRTTRGFPTNGVVAL
                     CDFGAGGTNVTLTNAGSNLAQIGPSVRDADFSGDAIDQLVLDHVQRTVADVTGADPAS
                     TTRIGSQSRLLGQCRRAKEQLSTATVTAVATGSGDDVRLSRTEFEQLISEPLDRFVGT
                     VEDTLQRNGIQRSQLSAVAIVGGGASIPLIITRLSGTLQVPIHTAPQPEFSAAIGAAV
                     LGERQASAGVPTTASPMVETPTEMVTAQVTERESALAWSQDADGADEPVPYTGPDMTG
                     EYGREATEFGAADGDRYPAATAALPWYKRTALVLSVAGAAGAVLVAVLLALTLGRTKN
                     NPVNNTPPNQPAPPQTSQTVTITGPNDTTTVTVIPAPPPSSTEPPVTTTTSQPPTTTT
                     TTTSAPPTTTSTATTTAPPTPTRERPPPFRPEPPFRR"
     gene            complement(3004..4518)
                     /gene="iniC"
                     /locus_tag="VFI55_09635"
     CDS             complement(3004..4518)
                     /gene="iniC"
                     /locus_tag="VFI55_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoniazid-induced dynamin-like GTPase IniC"
                     /protein_id="HET7074323.1"
                     /translation="MSTSDQVRAILGGTIRAYRSEPEYRQRADVFHELDRIGARLAEP
                     IRIALAGTLKAGKSTLVNALVGEDIAPTDATEATRIVTWFRHGPTPKVTANHWGGRHS
                     NVPINRSDGLTFDFRTLNPGDVVDLDVEWPAQELVNTTIVDTPGTSSLTRDASERTLR
                     LLVPPDGVPRVDAVVFLLRTLNAADVALLKQIGHLVGGSAGALGIIGVASRADEIGAG
                     RIDAMLSAADVAKRFTSEMNQTGICQAVVPVSGLLALTARTLRQSEFIALQKLAGADS
                     AALSKAMLSVDRFVRPDSLLPVDAATRAQLLERFGMFGIRISIGVLAAGVTDAAGLAD
                     ELLERSGLVALRQVIDQQFAQRSDMLKAHTALVSLRRFVEAFPIVATPYIIADIDPLL
                     ADTHAFEELRLLSQLHSRPTTLNEDEIFSLRRVIGGSGTDPASRLGLNPQDPYGPEGA
                     RAAFAAAQRWRRRAEHPLNDPFTTRVCRAAVRSAEAMVADFAARGYDMSNVQPR"
     gene            complement(4515..6356)
                     /locus_tag="VFI55_09640"
     CDS             complement(4515..6356)
                     /locus_tag="VFI55_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015292084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin-like GTPase family protein"
                     /protein_id="HET7074324.1"
                     /translation="MTQPDDPRRVNVIIELIDHTAAIAELNDRDDLAQRLVRARERIL
                     DPRIRVVIAGQLKQGKSQLLNSLLNLPVARVGDDEATVVITVVSYGDPPSARLIVAAG
                     PNGEPAAVDIPVDDINTDLRRAPQASGREVLRVEIGAPSPLLQGGLAFVDTPGVGGHG
                     QPHLSATLGLLPDADALLMVSDTSQELTEPEMRFLRQAHQICPVAVLAATKTDLYPHW
                     REIVNANAGHLQRAGVPIPILPVSSLLRSHAVTLNDKELNDESNFPAIVKFLSEKVLS
                     RETDRVRDQILSEIRSAAEHLATSVGSELAVVDDPDLRDRLADDLERRKRDAQDALNQ
                     SALWQQVLNDGFTDLYADVDHDMRARFRTLTEDIERQIDSCDPTRHWAEIGTDVENAV
                     ATAVGDNFVWAYRRSEALADDVAQSFLDAGLDSVMSPELTGRLMGGDFGRLKSLAELE
                     SKPAGKGQKVVTGLRGSYGGVVMIGMLSSVAGLGLFNPLSVGAGLVLGRMAYKEDKEN
                     RLLRARAEAKTNVRRFVDDVSFVVGKESRDRLKTIQRTLRDHYRDIGNEITRSLNESL
                     QATIAAAHIEEAERDNTIRELERQLNILEQVIDNVDKLTPPVTVGRS"
     gene            6846..7022
                     /locus_tag="VFI55_09645"
     CDS             6846..7022
                     /locus_tag="VFI55_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074325.1"
                     /translation="MATDAGAAALNDRGLPARSDRAVLGRETPAVDGAATAQCGAFLT
                     ENLAMGSATGTTGD"
BASE COUNT         1158 a         2492 c         2417 g         1134 t
ORIGIN      
        1 ctgctgcccg ccgccgctga tgaggtatcc gcgagtatcg cgcacctgtt ctccgcgcac
       61 gcgcaggact atcaggtagt ggccgggcaa gcggcggcat ttcatcagca gttcatgcag
      121 aacttgactg ccggcgcggg agcgtatgcc agcgccgaga ccgccaacgc cgcggtgctg
      181 cggcctttga atgcaatcgc gggctcggtt gccagcgctg cggcgcccat aaacccactg
      241 acgccattga cggagtggta tgacaccgtc atgagccaat tgcagaacgc aatcggcggc
      301 ttgctcttct ttttgttgtc accaatcctg accccaatta tcgacgcaat agctaaggct
      361 cttgtcgacg catttattga agcattgctc ggccaatcgt caactacttg agcggcccag
      421 gcacacgctg ctttgacccc caggaacgtc cgtcggcatc gacacgttcg acgcgcccca
      481 agctcacaac caggtggcgt cagtaccatc ggtgccgtgg cacgcactgg cccgccggac
      541 gagttccacc acccgcagac gcaatatccc aactacggga tgggcgggta tccgccgccg
      601 gctgtacccc cggggccccc tcccgagcct ccgccagagc ggcaggggcc acccgactat
      661 ctcgacccgc tcgaccagga agtcgagccc accccgtggt accgcaagcc cgcggggctc
      721 atctcgtggc ttgtcgccgt tctgatcctg atcggcctca tcatctacgg catcatcgag
      781 ctcattcagg gcgagggcgg cgggtatacg ccgggcacca gcacgaccac gacccccgcc
      841 acggtaactt ccacgacgcc ctccacgacg ccaccgacca gcagtgcgac gaccacgacg
      901 cctaccgcgc cgcccaccag cagcgcggtc gagccgccga cgcgtcagcc aacccagcag
      961 cagcccaccc accggcatca cctgccgccc ctgccgtcgg tgatcaccat tcccgaggtg
     1021 ccgaccgtga tcaccctgcc acccggcctc accgactaag ccggtcgcgg tcagatcacg
     1081 ctttggctgc tgaacggtag cgacgcgcgt ggcgacggaa tgcgtctggt cggcgccgat
     1141 agcgtcggcg gtcatggagc cgatcgcgac ctggtgcgaa ctgatcgcct tgatcggggc
     1201 attcctatac ctgcccttgg tgatcacgcc gccgtcgccg cccgccgtga tcgcactgcc
     1261 gcaaggcctg tgagccggcc tcgctcctag actcgctgaa agccgcaacc gttatggtga
     1321 cgaccgccgc gcgatcgccg gcaagccaac agacacaggg gtgtgcgcca tgagcgagtc
     1381 gctcgggttg tcgatcgggg tggccaacct ggttgcggcg cgcgtgggta gcgccccggt
     1441 gacccgcagt tccgtggtga cgctgttcga gcaccggccg accgaagccg gtctgccgga
     1501 agagaacccg aacctgaccg aaccggggct ggtgctgcgg ggattcgtcg agcgggtcgg
     1561 cgaccgggcg ccgctggtgg cggccgacgg gacgaagtac gccggggcgg cgctgaccgt
     1621 cgaagcgatc gaagcgatgg cacgcaccgt cggctacgga acgccggtca ccatcgcgat
     1681 ccccgcctat tggtccgagg cacagtccag cgcgctgcgc gaggagtttt tcgcccagcc
     1741 ggacctggcg cgcgccgggg tgcctccggt tttggtctcc gacgccacgg ccgcgctcgc
     1801 cgcgcttcgc accacgcggg gattcccgac caacggcgtt gtcgctttgt gtgatttcgg
     1861 agccggcggc accaacgtca cgctgaccaa cgccgggtcg aatcttgcgc agatcgggcc
     1921 gtcggtgcgc gacgccgatt tctctggcga cgcgatcgat cagctcgtcc tcgatcacgt
     1981 gcagcgcact gtcgccgatg tcactggcgc cgatcccgcg agcaccacgc ggatcggctc
     2041 ccagtcccgc ctgctcggcc aatgccgccg cgccaaagaa caactgtcga cggcgacggt
     2101 gaccgcggtt gcgacggggt cgggagacga tgtccggctg tcccgcaccg aattcgagca
     2161 actcatctcc gaaccgctgg accgattcgt cggcaccgta gaggacacgt tgcagcgcaa
     2221 cgggattcag agatcccaac tgtcggcagt ggcaattgtc ggcggcggcg ccagcattcc
     2281 actgatcatc acgcgcctgt cgggcacgct gcaggtaccg atccacaccg cgccgcaacc
     2341 ggagttcagc gccgccatcg gcgcggcggt gctcggcgag cggcaggcgt cggcgggcgt
     2401 tccaaccacc gcgagcccca tggtcgagac cccgaccgaa atggtcaccg cccaggtgac
     2461 cgagcgcgaa agcgcgctgg cttggtcaca ggacgccgac ggcgcggacg agccggtgcc
     2521 ctatacggga cccgacatga ccggcgaata cggccgggaa gcaacggaat tcggcgctgc
     2581 ggacggcgac cgctaccccg ccgcgacggc ggcgctgccg tggtacaagc gcacggcgct
     2641 cgtgttgagc gtggccggcg cggccggggc cgtgctggtg gcggtgctgt tggcgctgac
     2701 gttgggccgc accaaaaaca acccggtcaa caacaccccg ccgaaccaac cggcaccacc
     2761 ccaaacgtcg caaacggtca ccattaccgg gccgaacgac accaccacgg tgacggtgat
     2821 ccccgcgccg ccgccgtcgt ccacggagcc gcccgtgaca acgacgacca gccagccccc
     2881 gacgacgacc actaccacca cgagcgcccc accgaccacg acatcgacgg caaccacgac
     2941 cgcgccgccc actccgacgc gggaaaggcc gccgcccttc cgccctgaac ccccgttccg
     3001 gcgttaacgc ggctgaacgt tggacatgtc gtaaccgcgt gcggcgaaat cggcgaccat
     3061 cgcctcggcg ctgcgcaccg cagcgcggca gaccctggtg gtgaacgggt cgttgagggg
     3121 gtgctcggcc cggcggcgcc agcgttgcgc ggcggcgaat gcagcgcgcg caccctccgg
     3181 cccgtaggga tcctgcggat tcaggcccag cctgctggcc gggtcggtgc ccgagccgcc
     3241 gatgacgcgg cgcagcgaga agatctcgtc ctcgttcaaa gtcgttgggc gtgaatgcaa
     3301 ttgactgagt agtcgcagtt cctcgaaggc gtgcgtgtcg gccagcagcg ggtcgatatc
     3361 ggcgatgatg tacggcgtcg ccacgatggg aaaggcctcg acgaatcggc gcaacgacac
     3421 cagcgcggtg tgcgccttga gcatgtcgga gcgttgcgcg aattgctggt cgatgacctg
     3481 acgcagcgcc accagcccgc tgcgctccag cagctcgtcg gccaggcccg cggcgtcggt
     3541 gactccggcg gccagcacgc cgatggaaat ccggatgccg aacatgccga accgctccag
     3601 caactgcgcg cgcgtcgcgg cgtcgacggg taacaggctg tcgggccgca cgaagcgatc
     3661 cacgctcaac atggccttgc tgagcgccgc gctgtcggcg ccggcgagct tctgcagcgc
     3721 gatgaactcg ctctggcgca gcgtgcgtgc ggtcagggcg agcagtccgg acaccggcac
     3781 caccgcctgg cagatgcccg tctgattcat ctcgctggtg aaccgcttgg ccacgtcggc
     3841 ggccgacagc atggcgtcga tgcgacccgc gccgatctcg tcggcgcgcg acgcgacacc
     3901 gatgatgccc agcgctcccg ccgagccgcc gacgagatgg ccgatctgtt tgagcagcgc
     3961 aacgtcggcg gcattaagcg tgcgcaacag gaacaccacc gcgtcgaccc gtggcacccc
     4021 gtcgggcggc accagcagtc gcagggtgcg ttcggaggca tcccgcgtca acgatgacgt
     4081 ccccggagtg tcgacgatgg tggtgttgac cagctcttgc gccggccatt cgacgtccaa
     4141 gtcgaccacg tcaccgggat tgagcgtccg gaagtcgaac gtcaggccgt cgcttcggtt
     4201 gatcggaacg ttggaatgcc gaccgcccca atggttggcg gtgactttcg gtgtcgggcc
     4261 gtgtcggaac caggtcacta tccgcgtcgc ctcggtggca tcggtcgggg cgatgtcctc
     4321 gcccaccaaa gcgttgacca gagtggactt tccggccttg agggtgccgg ccagcgcgat
     4381 gcggatcggt tcggccaggc gcgcgccgat gcggtccaat tcgtggaaga cgtccgcgcg
     4441 ctgccggtac tccggctcgc tccggtaggc ccggatggtt ccgccgagaa tcgcgcggac
     4501 ctgatcgctg gtgctcacga ccgtcctacg gtcaccgggg gcgtcagctt atccacattg
     4561 tcgatgacct gctccaggat gttgagttgc cgctccagtt cacgaatcgt attgtctcgc
     4621 tcggcttctt cgatgtgcgc cgcggcgatg gtggcctgca gtgattcgtt gagcgagcgg
     4681 gtgatctcat tgccgatgtc gcggtagtgg tcgcgcagtg tgcgctggat ggtcttgagc
     4741 cgatcgcgcg actccttacc gaccacgaac gacacgtcgt cgacaaagcg ccgcacgttg
     4801 gttttggcct cggcgcgtgc ccgcagcaga cggttctcct tgtcctcctt gtaggccatg
     4861 cggcccaaca cgagcccggc ccccaccgac agcgggttga acaggcccag cccggctacc
     4921 gacgacagca tgccgatcat caccacaccg ccgtaggagc cccgcagtcc ggtgaccacc
     4981 ttctgcccct taccggccgg ctttgattcc agctcggcca gggacttgag tcgtccgaaa
     5041 tcgccaccca tcagacgccc ggtcagttcg ggagacatga ccgagtccag gcccgcgtcg
     5101 aggaacgact gggccacgtc gtcggccagg gcctcagacc gccggtaggc ccacacgaag
     5161 ttgtccccga cggcggtcgc gaccgcgttc tccacgtcgg tgccgatctc agcccaatgc
     5221 cgggtcgggt cacacgaatc gatctggcgc tcgatgtcct cggtgagggt gcggaagcgg
     5281 gcgcgcatgt cgtggtcgac gtcggcgtac aggtcggtga acccgtcgtt gagcacctgc
     5341 tgccacagcg cggactggtt gagggcgtcc tgcgcatccc gtttgcgccg ctccagatcg
     5401 tcggcgagcc ggtcacgcag gtccgggtcg tcgacgaccg ccagctcgga acccaccgaa
     5461 gtggccaaat gttctgccgc cgagcggatt tcggagagaa tctgatcgcg cacccggtcc
     5521 gtctcgcggg acagcacctt ctcgctgagg aacttgacga tcgctgggaa attggactcg
     5581 tcgttgagtt ccttgtcgtt gagcgtgacg gcgtggctgc gtaacagcga cgacaccggc
     5641 aggatcggaa tcggcacgcc cgcgcgctgt aggtgaccgg cgttggcgtt gacgatctcg
     5701 cgccagtgcg ggtacaggtc ggttttggtg gccgcgagca cggcgacggg gcagatctgg
     5761 tgcgcctggc ggaggaaccg catctccggt tcggtcaact cctggctcgt gtcgctgacc
     5821 atcaacaggg cgtcggcatc cggcagcagc ccgagcgtcg ccgacaagtg cggctgcccg
     5881 tgaccaccca cgccgggggt gtcgacgaac gccagcccgc cctgcaggag cgggctgggc
     5941 gcaccgatct cgacgcgcag cacttcgcgg ccgctggctt gcggggctcg ccgcagatcg
     6001 gtgttgatgt catcgaccgg gatgtcgacg gcggcgggtt cgccgttggg tcccgcggcc
     6061 acgatcagcc gagccgacgg cgggtcgcca tagctgacga cggtgatcac caccgtggcc
     6121 tcgtcgtcgc cgactcgcgc cacgggcaag ttcagcagcg agttgagcaa ctggctcttg
     6181 ccttgtttga gctgcccggc gatcaccacc cggatacgcg gatcgagaat tcgctcgcgc
     6241 gctcgcacca gtcgctgggc gaggtcatcg cggtcgttga gttcggcgat cgcggcggtg
     6301 tggtcgatca attcgatgat gacgttgacc cgccgcgggt catcgggttg ggtcaccgta
     6361 ctgccccact ttctcgctgc gatgcactga gatcacggta ttcgcgaaac ggcggggacc
     6421 aaacggatcc ccgccgactc gccagggtgc ccggatcaga atccgtgcgg ccctgctgga
     6481 tgtgttgccg gcgccgggtg aggtgccggt gcgggagcat gcaccggtgc gggctcgtgc
     6541 ggcgtgccct caaacaccga cgggctgtgc ggagccgggg ccggcgagtg ctcaaccgga
     6601 gcgggcgcgt gctgaaccgg agccggcgcg tgctgaaccg gagccggagc aggcgcgtgc
     6661 tgaaccggag cgggcgcatg ctgaaccgga ggctgagtag gtgcgtgagc cggagcctga
     6721 gcaggtgcgt gagccggtgc ctgtgcgggc tgctggatca ccggtgtgcc tgcgccgccg
     6781 tggctggtcg gtccaccgcc acccgggctc tggatcaccg ggccggggtt ggacggagga
     6841 gccgcgtggc caccgacgcc ggcgccgccg ccctgaatga ccggggcttg cccgctcgga
     6901 gcgaccgggc tgtgctcggc cgggagaccc ccgccgttga cggggccgcc accgcccagt
     6961 gtggcgcctt cttgaccgag aatctggcca tgggctccgc caccggcacc accggcgatt
     7021 gagccgccag ccggggtgcc gccgccgggg ctctggatta ccgggccggg gttcgacgga
     7081 ggagccgcgt ggccaccgac cccggcgccg ccgccctgaa tgaccggggc ttgcccgctc
     7141 gggacgaccg ggctgtgctc ggccgggaga cccccgccgt tgacggggcc gccaccgccc
     7201 a
//
LOCUS       DASSGQ010000222         7145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_714184,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000222 DASSGQ010000000
VERSION     DASSGQ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714184"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2342)
                     /locus_tag="VFI55_09650"
     CDS             complement(<1..2342)
                     /locus_tag="VFI55_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HET7074326.1"
                     /translation="MTTQMLIRLVVGLGMTAIVGALSVRRVFWLYKLVMSGQPASGRT
                     DNVGTRIWTQIVEVFGQRRLLKWSIPGLAHFFTMWGFFILLTVYIEAYGFLFQENFHI
                     PIIGRWDALGFLQDFFATAVFLGISTFAIIRLRSEPKDYGRQSRFYGSHTGGAWLILF
                     MIFNVIWTYVLVRGSAVNNGTLPYGKPAFLSQLFGAILRPLGQPANEIIETTALLLHI
                     GVMLGFLLIVLHSKHLHIFLAPINVTFKRLPDGLGPLLPVESGGKPIDFENPPDDAEF
                     GRGKIEDFTWKGMLDFATCTECGRCQSQCPAWNTGKPLSPKLVIMDLRDHWMAKAPYI
                     LGDKETPLENTPEGGVGEDLGGEKHAEEHHVPESGFGRVLGSGPKQATRPLVGTAAQL
                     GVIDPDVLWSCVTCGACVEQCPVDIEHIDHIVDMRRYQVMMESEFPSELSVLFKNLEA
                     KGNPWGQNAADRTNWIDEVDFDVPVYGEDVDSFDGFEYLFWVGCAGAYDDKAKKTTKA
                     VAELLAIAGVKYLVLGTGESCNGDSARRSGNEFLFQQLAAQAVETLDGLFEGVETVDR
                     KIVVSCPHCFNTLGKEYRQLGSNYTVLHHTQLLNRLVRDKKLVPVSSVSQDITYHDPC
                     YLGRHNKVYEAPRELIGAAGAKLTEMPRHAERSFCCGAGGARMWMEEHIGKRINHERV
                     DEALATNATTIATGCPFCRVMVTDGVNDRQEEAGRSGVEVLDVAQVLLGSLDSDQAKL
                     PAKGTAAKEAAQRAPKAAPEPKPEPTAAALAEAPERPAEQP"
     gene            complement(2481..4883)
                     /gene="iniR"
                     /locus_tag="VFI55_09655"
     CDS             complement(2481..4883)
                     /gene="iniR"
                     /locus_tag="VFI55_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003913118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoniazid response ATPase/transcriptional
                     regulator IniR"
                     /protein_id="HET7074327.1"
                     /translation="MSDYPVDVPRGAREAGQVLAGAGTAPIKLLISGGVGTGKSTVLG
                     AARETLRGAGLTVLSRPPGAGDPPDAALVIDDVQLLTDAELRAVIERVADPRATVVLA
                     AEPQEQLRDLTVAIERDRARILLGPLPVAEHLMDHTAGLPFLVRAVSDGAQPPALAAK
                     FALIERLRRLDELTLDTLLIMSLTQESGTADVAAALGISATDARQLVDRARASGLIEP
                     SHRPQFLQLVHNAVAQIVGTAHHHEVEAALLRSQLDMSTMSPDLALRLAEHGLKDGRL
                     ADTLARQATAARGDSVGAARLYRAAVDAGAEGLASRVADALALTGDTGAAAALADELL
                     SSPDSAERAAAVRIAASVAAQDGNSNQAAELFGWLGPHPDAVVGAAAAIVLAATGDLA
                     AARVALRLKDTGPPTMAARTARSLAEGLLLTMDHPYPAAMAKLGQAIAAEQSLGEVLP
                     DSPAALVTLAAVHGGDPVRARSVIGRAVRADGDVLFVRRHALLSSWIKMQDGQLPVAG
                     ADIGAAGPVEVHRRDALWAAALHTAIARRSGDAGALHKHWYAAMEVLAEYSVDLFSLL
                     PLGELWVAAARMRQVDQLRHALEQAFGLLDALGNPALWSVPLHWAGVHAGILANSPES
                     VAPHGQALSAAAGDSALARTLSVAGRTWLRVLANHVDADEVTGAARSLSNVGLTSDAT
                     RLAGQAALQTPDGRVSGAMLQLARDLKLGASPGPTPAEPATGAASVTHQPAAGSALSD
                     REREVAELLLLGMPYRDIGGQLFISAKTVEHHVARIRRRLGAGSRSEMISMLRAMLAP
                     DA"
     gene            5097..5645
                     /locus_tag="VFI55_09660"
     CDS             5097..5645
                     /locus_tag="VFI55_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003901836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv0340 family IniB-related protein"
                     /protein_id="HET7074328.1"
                     /translation="MANSLLDFVISLVRDPDAAARYAANPAQAIADAHLTNVTSADVN
                     NLIPMVSDSLSMSNPAGGISGAPVTDHGNVWASGAAAAALDAFSSHPPVGVVDQRDQA
                     HGVIDQPSMPGGPGSAADPHPFVADPHQPSVLLTSADVPDSTIDHGGFPADDSSLWDH
                     STHAAAHPHTVEPDHHGFGLHG"
     gene            complement(5888..>7145)
                     /locus_tag="VFI55_09665"
     CDS             complement(5888..>7145)
                     /locus_tag="VFI55_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074329.1"
                     /translation="RLTTNTQTSTTTQTSRTAEPSLTGQAHPATDTSTSTKTRLTSNT
                     QAGLTAEPKAQPATDLPTKTRTATQTNTCLSTQTSTKPKVQAGPDVGTQTSPTAEPES
                     ETQPTTDLSIQASAGTETAAKRLAQKRTWTEPTPTRPATRTEATPAGATTRPKTGPRT
                     TPTRPGVAPTRPRLTPTMPETTPTGPRTIPTRPKSRPRPIPTMPKTGPRLTPTMPKAV
                     PTRSETGSEPARARSECAPTGLPTEPTVTGVPGPGAEAISQPPVKPVRDGDTVAQSNV
                     CAVHDRGDDVTHQRGAVFGDRGDRALVVAAIARVVAAVARVVAAVALVVRPVTRVVAP
                     ITRVVPILVGQDLLQTTDRVASEVRSGHRISRRHCKLVRRHVDQAGVAHGLIGAIEEG
                     LSRFRVGKQVQDVSDQRVHDRFPSNL"
BASE COUNT         1027 a         2312 c         2550 g         1256 t
ORIGIN      
        1 ggctgctcgg cgggtctctc tggagcctcg gcaagtgcag cggccgtggg ttccggcttc
       61 ggctcggggg ccgccttcgg ggcgcgctgc gccgcctctt tggcggccgt gcccttcgcc
      121 ggcagcttcg cctggtcgga gtcgagcgac ccgagcagca cttgggccac atcgagcacc
      181 tcaacaccgc tgcgaccggc ttcttcctgc cgatcgttga caccgtcggt gaccatgacc
      241 cggcagaacg ggcagccggt ggcgatcgtg gtggcgttgg tggccagcgc ctcgtcgacg
      301 cgttcgtggt tgatccgctt gccgatgtgc tcttccatcc acatccgggc gccgcccgcg
      361 ccgcagcaga agctgcgctc ggcgtgccgc ggcatctcgg tgagtttcgc cccggcggcc
      421 ccgatcaact cccgcggcgc ctcgtacacc ttgttgtgcc gacccaggta acacgggtcg
      481 tgataagtga tgtcctggga gacggagctc acgggaacca gcttcttgtc gcggaccagc
      541 cggttgagca gctgggtgtg gtgcagcacg gtgtagttgg agcccagctg ccggtattcc
      601 ttgccaaggg tgttgaagca gtgcgggcag ctgacgacga tcttgcggtc gacggtctcc
      661 acgccctcga acagcccgtc cagcgtctcg acggcctgcg ctgccagctg ctggaacagg
      721 aactcgttgc ccgagcggcg cgccgagtcg ccgttgcagg attcgcccgt gcccaacacc
      781 aggtacttca ccccggcgat ggcgagcagt tcggcgacgg ccttggtggt ctttttcgcc
      841 ttgtcgtcgt aagcgcccgc gcagcccacc cagaacaggt actcgaagcc gtcgaagctg
      901 tccacgtcct cgccgtaaac cgggacgtcg aagtcgacct cgtcgatcca gttcgtccgg
      961 tccgcggcgt tctggcccca cgggttgccc ttggcctcca ggttcttgaa cagcaccgac
     1021 agctcagagg ggaactccga ctccatcatc acctggtagc ggcgcatatc gacgatgtgg
     1081 tcgatgtgct cgatgtccac cggacactgc tcgacgcacg cgccgcaggt cacacacgac
     1141 cacagcacgt cgggatcgat gacgccgagc tgtgcggcgg tgccgaccag cgggcgggtc
     1201 gcctgtttcg gcccggagcc gagcacgcgg ccgaagcccg actcggggac gtggtgttcc
     1261 tcggcgtgct tctcgccgcc caggtcctcg ccgaccccac cctccggggt gttctccagc
     1321 ggggtctcct tgtcgcccag gatgtagggt gccttggcca tccagtggtc gcgcaggtcc
     1381 atgatgacga gcttgggcga cagcggcttt ccggtgttcc aggccggaca ctgcgactgg
     1441 cagcgcccgc actcggtgca ggtggcgaag tcgagcatgc ccttccaggt gaagtcctcg
     1501 atcttgccgc ggccgaattc ggcgtcgtcg ggcgggtttt cgaagtcgat cggcttgccg
     1561 ccggactcga ccggcagcag cggacccaac ccgtccggca gcctcttgaa cgtgacgttg
     1621 atgggcgcca ggaaaatatg caggtgcttg gagtgcagca cgatcagcag gaagcccagc
     1681 atgacaccga tgtgcagcag cagggccgtc gtctcgatga tctcgttagc gggctgacca
     1741 agcggacgca ggatggcgcc gaacagctgc gacaggaacg ccggctttcc gtagggcagt
     1801 gtgccgttgt tgaccgcgga tccgcggacc agcacatagg tccagatgac gttgaagatc
     1861 atgaacagga tcagccacgc gccgccggtg tgcgagccgt agaagcggga ctggcgacca
     1921 taatctttcg gctcgctgcg gagccggatg atggcgaaag tcgagatgcc gaggaagacg
     1981 gcggtggcga agaagtcctg caggaagccc agcgcgtccc agcggccgat gatcggaatg
     2041 tggaagttct cctggaacag gaacccgtac gcctcgatgt agaccgtgag caggatgaag
     2101 aagccccaca tggtgaagaa gtgcgccaac cccgggatcg accacttcag caggcggcgc
     2161 tgcccgaaaa cctcgacgat ctgtgtccag atccgggtgc cgacattgtc ggtgcgcccg
     2221 ctggccggct ggccggacat caccagcttg tacagccaga aaactcgtcg caccgagagg
     2281 gcgccgacga tcgcggtcat gccgaggccc actaccagtc tgatgagcat ctgcgtggtc
     2341 acagaaggtc tccccaatgc atagttattt cggcgtgctt attggctgct gccaggtttg
     2401 ctcatcgtta catcttcccg ggtttcgatg cgagaaaggc tgtcctaact tcctgttcat
     2461 cggtgccctt ggcctgcgct tcaggcgtcc ggggccagca ttgcccgcag catggagatc
     2521 atctcggaac gcgagccggc gcccagccgg cgccgtatcc gggcgacgtg gtgctccacc
     2581 gtcttcgccg agatgaacag ttggccgccg atgtcgcggt acggcatgcc gagcagcagc
     2641 agctcggcga cttctcgttc gcgatccgac agcgctgacc ccgccgcggg ctggtgggtc
     2701 accgacgcgg caccggtcgc cggttcggcg ggcgtcgggc cggggctggc gcccaacttc
     2761 aggtcgcggg ccagttgcaa catggccccg gacacccggc cgtcgggtgt ctgcagcgcg
     2821 gcctgaccgg ccagccgggt cgcgtccgag gtcagcccga cgttcgacaa cgaccgggcc
     2881 gcgccggtga cctcgtcggc gtcgacgtgg ttggccagca cccgcagcca cgttcggccg
     2941 gcgacggaca gcgtccgcgc cagcgcgctg tcgccggcgg cggcgctgag ggcctgcccg
     3001 tgcggcgcga ccgactcggg tgagttggcg aggattcccg cgtgcacgcc agcccagtgc
     3061 agcggcaccg accacaacgc cggattgccc agcgcgtcga gtagcccgaa cgcctgctcc
     3121 agggcatggc gcagctggtc gacctggcgc atgcgggcgg ccgccaccca cagttccccg
     3181 agcggcagca gcgaaaagag gtcgacggaa tactcggcga gtacctccat ggccgcgtac
     3241 caatgcttgt gcagcgcacc cgcgtcgccg ctgcggcgcg cgatcgcggt gtgcagcgcc
     3301 gctgcccata acgcgtcgcg tcggtgcacc tccacggggc cagcggcgcc gatgtccgcg
     3361 ccggccaccg gcagttgccc gtcttgcatc ttgatccagc tggacagcaa cgcgtgccgg
     3421 cgcacgaaga gcacgtcgcc gtcggcgcgc acagcccggc cgatcacgct acgggcgcgg
     3481 acgggatcgc cgccatggac ggcggccaag gtcaccagcg cggcggggct gtccggcagc
     3541 acttcgccga gggattgttc ggccgcaatg gcctggccca atttcgccat tgccgccggg
     3601 tagggatggt ccatggtcag cagcagccct tcggcgaggc tccgcgcggt gcgggcggcc
     3661 atcgtcggtg ggccggtgtc cttgagccgc agggcgaccc gtgccgccgc aagatcgccc
     3721 gtcgcggcga gcacgatcgc tgcggccgcg ccgaccacag cgtccgggtg gggtcccagc
     3781 cagccgaaca gttcggcagc ctgatttgag ttgccgtcct gggcggcgac gctggcggcg
     3841 atccggaccg ccgcggcgcg ttcggccgaa tccggcgagc tgagcagttc gtcggccaag
     3901 gcggccgcgg ccccggtgtc tccggtgagg gccagggcgt cggcgactcg cgacgcgagc
     3961 ccttcggcgc cggcgtcgac cgccgcccgg tatagcctcg cggccccaac ggagtcaccc
     4021 cgtgccgccg tggcttgccg agccagggtg tccgccaacc ggccgtcttt cagcccgtgc
     4081 tcggccaatc gcagcgccag gtccggcgac atggtcgaca tgtcgagttg cgaacgcaac
     4141 agggcggctt cgacttcgtg gtggtgcgct gtgccgacga tctgggcgac cgcgttgtgc
     4201 acgagctgca ggaattgggg gcggtgcgac ggttcgatga gtccgctggc gcgcgcccga
     4261 tcgaccagtt ggcgcgcgtc tgtggccgaa attccgaggg cggccgccac atcggcggtc
     4321 cccgattctt gggtcagcga catgatcagg agggtgtcca aggtgagctc gtcgagccgg
     4381 cgcaatcgct cgatcagcgc gaacttggct gccagtgccg ggggctgcgc cccgtcgctg
     4441 accgcgcgca ccaggaacgg cagccctgcg gtgtgatcca tgaggtgttc ggcgaccggg
     4501 agcggaccca gcaatatgcg tgcccggtcc cgctcgatgg ccacggtcag gtcccgcagc
     4561 tgctcctggg gctccgcggc gagcacgacc gtcgcgcggg ggtcggccac acgctcgatg
     4621 acggcgcgca gttcggcgtc ggtgagcaac tggacgtcgt cgatcaccaa cgccgcgtcg
     4681 ggtggatcgc cggcgccggg cgggcgtgat agcaccgtca gcccggcacc gcgcagcgtt
     4741 tcccgagccg cgccaagcac ggtgctcttg cccgtgccga cgcccccact gatcaggagc
     4801 ttgatcgggg cggtcccggc gccggcgagg acctggccgg cttcacgggc gccccgtggg
     4861 acgtcgacgg ggtagtcact cacgtcgcgt acccccttgg ttcgggcccc aatgggcggt
     4921 cagccatccc cgtagcccca tcattcccct aatgaggcac cccctaatga tgccgcaccg
     4981 caggtgggtt gcgcaccgat cacacgcgcg gcggcgatgg ctaccatctt ggtagcaggc
     5041 tgcgctttcc tgtggatgtc ggcggcatgg gaaacggcat gggacggagg tgcgcgatgg
     5101 caaactcatt gcttgacttc gtgatctcgc tggtacgcga tcccgacgcc gccgcgcgtt
     5161 acgccgccaa ccccgcgcag gcgatcgccg acgcccatct tacgaatgtg accagcgcag
     5221 acgtgaacaa tctgatcccg atggtgtcgg actcgctgtc gatgtccaat cccgccggcg
     5281 gtatctcggg agcgcccgta accgatcacg gcaacgtatg ggcgagcggc gcggccgccg
     5341 ctgcgctcga tgcgttttcg tcacacccgc ccgtcggggt tgtcgaccag cgcgatcagg
     5401 cacacggcgt aatcgaccag ccgtccatgc ccggaggccc cgggtcggca gccgatccgc
     5461 atccgttcgt ggccgacccg catcagccgt cggtgctgct caccagcgcc gatgttcccg
     5521 acagcaccat cgaccacggc gggttcccag ccgacgattc gagtctgtgg gaccactcca
     5581 cccacgcggc ggctcacccg cacaccgtcg agccggatca ccacggcttc ggcctccacg
     5641 gctgactttc cttttgttga cggcggcctg cagggtcgcc gataagcctt tgacgtgctc
     5701 atatccggtc aattaggggt agctgccgcc tgcgtgcggg gcagcgcgca atcccctaac
     5761 cccctaatgg ggtagcccga tcgatcccga tgggcgcttg ggcttttggg ggatcgagcc
     5821 ccgattcggg ccagtcgtga aatccatagc gtgtatatgg aaacgccggt tggcccggcg
     5881 aacggcttca gagatttgag gggaaccgat catggacgcg ctgatccgat acatcctgga
     5941 cctgtttacc aaccctgaag cggctcaggc cttcttcaat ggccccaatc aggccatgag
     6001 cgacgccggc ttggtcaacg tggcgccgga cgagtttgca atggcggcgg ctaatgcgat
     6061 gcccggaacg gacttcggag gcgacccggt cggtggtctg cagcaggtcc tggccgacca
     6121 gtatgggtac gacccgggta atgggggcta cgacccgggt tacgggccgt acgaccaggg
     6181 ctacggcggc tacgacccgg gctacggcgg ctacgacccg ggctatggcg gctacgacca
     6241 gggcccggtc gccacggtcg ccgaagacgg cgccgcgctg gtgggtgacg tcgtcgcccc
     6301 ggtcgtgtac ggcgcagacg ttgctctggg cgacggtgtc gccatcgcgg acgggtttga
     6361 cggggggctg gctaatggct tcggccccgg gccctgggac gccggtgacg gtgggttcgg
     6421 ttggcagccc ggttggggcg cattcggacc tggcacgggc gggttcggac ccggtttcgg
     6481 acctggttgg gacggctttg ggcatggttg gggtgagcct cggcccggtt ttgggcatgg
     6541 ttgggatggg cctcggcctg gatttgggcc tggttgggat ggtcctcggc ccggttgggg
     6601 tggtttcggg catggttggg gtgagcctcg gcctggttgg ggcgaccccc ggcctggttg
     6661 gggtggttct gggcccggtt ttgggacggg ttgtggcccc ggctggggtg gcttcggtcc
     6721 gggttgcggg cctggttggg gtgggttcgg tccacgtccg cttctgggcg agccgcttgg
     6781 cggcggtttc ggtgccggcg ctggcttgaa tgctgagatc agtggtgggt tgggtctcgg
     6841 attcgggctc ggcggtgggg ctggtctgag tgccgacatc ggggccggct tgaaccttgg
     6901 gtttggtgct ggtttgggtg ctcagacagg tattggtttg ggtggccgtg cgggtcttgg
     6961 tggggagatc ggtggcgggt tgggccttgg gttcggcggt cagaccggct tgggtgttgc
     7021 tggtgaggcg ggtcttggtg ctcgtgctgg tgtcggtggc ggggtgggcc tggccggtga
     7081 ggctgggttc ggcggtgcgg ctggtttggg tggtcgtgct ggtttgggtg ttggtggtga
     7141 ggcgg
//
LOCUS       DASSGQ010000223         3557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_805515,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000223 DASSGQ010000000
VERSION     DASSGQ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805515"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..1410
                     /locus_tag="VFI55_09670"
     CDS             271..1410
                     /locus_tag="VFI55_09670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET7074330.1"
                     /translation="MPEAVIVSALRTPIGTARKGTLRDTNAFDLAHHVVTEAAKDLDP
                     AQVDDVILGEGLYGGGVVARHAAITAGLPQVPGLANNRHCAAGQAAVQSAAASVRAGM
                     DQLVIAGGVNSASTSPRSRFQVDGEWVDWFPPTHPDRPDAPNLDMSITVGWNAAVKAG
                     VSREEMDAWALRSHRNAIAAIDEGRFKEEIVPIETPHGLFSVDEHPRRETSMEKLAAL
                     KPLHPEIEGFSITAGNACGANDGAAALTIASAALGLPALATVRSWASVGVDPAITGLA
                     PVQAIPKALARGGLSIPDVDVFEINEAFAAMCVATIKLLDLDPERVNVSGSGCSLGHP
                     VAATGARMLVTLVYELRRRGGGIGVAAMCAGGGMGSATVIEVAAP"
     gene            1412..2308
                     /gene="meaB"
                     /locus_tag="VFI55_09675"
     CDS             1412..2308
                     /gene="meaB"
                     /locus_tag="VFI55_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014710965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET7074331.1"
                     /translation="MSIADLIAGARNGSPRAAGRLLSLVESDRRDEVLASIGPSPARA
                     GPVIGITGPPGSGKSTTIAGLVGAYRRRGCRVAVLAVDPSSPFSGGALLGDRIRMTVH
                     INDSDVLIRSVATRGHLGGLAAAVPAAIRLLGAIGYGVVLLETVGVGQSEIEIAAVAD
                     PTIVILTPGAGDAVQAAKAGVLEVADIVAVNKADREGAEQTVRDLRAELGPAGAPIVS
                     LIAARGEGITDLMAAIDDHHRADSRARRLARARAQILSLAQTRLRTRPDLDRLAEAVV
                     DGRDDPYSAAERLLSSTPQPED"
     gene            complement(2355..3155)
                     /locus_tag="VFI55_09680"
     CDS             complement(2355..3155)
                     /locus_tag="VFI55_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074332.1"
                     /translation="MAAGVEYLHRTDLVGTGTDLASVLRTWLDSEPGTATVVAPPGTD
                     LRSMRVAWALPPAVDLVTDLRGDLTRGSSLALLSPDEAMLDAAAFYLNTTSRVLVVLG
                     NDGPTITGWLRKHGATHIKDRNALAPLNGVLAEPAVEYGLRYLLSLLVRGFASDAFGR
                     EPTKSKAISAFQAFRRGGVPLEAGPLLLEFLVSNGATISDAITLNDYARHVTDDAEIP
                     TRAANRFRDDILQVWRDAAGPASNDLITVDRVSRRGDNDPPESTQACG"
     gene            complement(3311..>3557)
                     /locus_tag="VFI55_09685"
     CDS             complement(3311..>3557)
                     /locus_tag="VFI55_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7074333.1"
                     /translation="VFDALVAIGLGQWQQLADSGATDADIDVVWAALNPLILTLGAII
                     LRRHLDRHLPEPFTTRTQLQRWEKAVEQLISRGQLRG"
BASE COUNT          599 a         1155 c         1207 g          596 t
ORIGIN      
        1 acgtgtccgc ccgggaccgt aattgtcccc tccatgccga cgatgcctcc tgggttggcg
       61 agcagtcccc gcaagcgggg gtccccagca gaatcgcacg attcgatgcc gggatatgcg
      121 attctgtgtc tgctcgggag ccgaccgacg cggaccgtct ccttgcaagg atgactctcg
      181 gcttgagaga atagtattct cgtgtctgtg cgggtggtgc ttgtgtcccg cacaggctcg
      241 tcgaagtgtt cgccgaactg gagaagacct gtgcctgaag ccgtcatcgt gtccgccctg
      301 cgcaccccca tcggcaccgc ccggaagggg acgctgcgcg acacaaatgc attcgacctt
      361 gcgcaccacg tggtgaccga agcggccaag gatctggacc cggcccaggt cgacgatgtg
      421 atcctgggag agggtctcta cggcggcggt gtggtcgccc gccacgcggc cattaccgca
      481 ggtctgcccc aggtgcccgg cttggcaaac aatcggcact gcgcggcggg gcaggcggcg
      541 gtgcagagcg cggcggctag tgtgcgcgcg ggaatggacc aactggtcat cgccggcggt
      601 gtgaactcgg catccacgtc gccgcggtca cggttccaag tggacggcga gtgggtcgac
      661 tggttcccgc cgacccatcc ggaccgtccc gacgcaccca atctcgacat gtcgatcacc
      721 gtgggctgga acgccgccgt caaagcgggc gtgagccgcg aggagatgga cgcgtgggcg
      781 ctacggtcac accgcaatgc gatcgccgcg atcgatgaag gccgcttcaa agaggagatc
      841 gtccccatcg agactccgca cgggctgttc tcggtcgacg agcacccgcg ccgagagacc
      901 agcatggaaa aactggccgc actcaagccg ctgcatcccg aaatcgaggg cttttcgatt
      961 accgccggca acgcctgcgg cgccaacgac ggtgccgccg cactgactat cgcgagcgcc
     1021 gcgctgggac tgcccgcgct ggccaccgta cggtcttggg catcggtggg cgtcgacccc
     1081 gcgatcaccg gattggcgcc ggtgcaagca ataccgaaag cccttgcccg cggcggtctt
     1141 tcgatccccg atgtggacgt gttcgaaatc aacgaggcgt tcgcggcgat gtgtgtggcc
     1201 accatcaagc tgctcgacct cgaccccgag agggtcaacg tcagcggtag cggctgctcc
     1261 ctgggacacc cggtggcggc gaccggggcc aggatgctgg tgaccctggt gtacgaactg
     1321 cgtcgtcgcg gcgggggcat tggcgtcgct gccatgtgcg ccggcggcgg catgggttcc
     1381 gcgacggtca tcgaggttgc ggctccgtga catgtccatc gccgacctga ttgcgggtgc
     1441 gcgcaacgga tctccgcgag cggccggtcg gttgctgagt ctcgtcgaga gcgatcgtcg
     1501 ggacgaggtg ctggcgagta tcgggccctc accggcacgc gcggggcctg tcataggcat
     1561 caccggaccg ccgggttcgg gcaagtcgac gacgatcgcc gggctggtcg gcgcctaccg
     1621 gaggcgtggc tgccgggtgg ccgtgcttgc ggtggacccg tcgtcgccgt tcagcggagg
     1681 tgcgttgctg ggcgacagaa ttcgaatgac cgtgcatatc aacgactctg acgtcctgat
     1741 ccgttcggtg gctactcgcg gtcacctcgg cggtctggct gccgcggttc cggcagcgat
     1801 ccgactgctc ggcgcgatcg ggtacggcgt ggtgctgctg gagaccgttg gcgtggggca
     1861 gtcagagatc gagattgctg ccgtcgcgga ccccaccatc gtcatcctca ctcccggcgc
     1921 gggcgacgcg gtgcaggcgg ccaaggccgg cgtgctggaa gtcgccgaca ttgtcgcggt
     1981 caacaaagcc gaccgggagg gcgccgaaca aacggttcgg gacttgcgcg ccgaattggg
     2041 ccccgccggg gccccgatcg tcagtctcat cgccgcccga ggcgagggca taacagatct
     2101 catggccgcg atcgacgacc accaccgcgc cgacagccgc gcgcgccggc tcgcccgcgc
     2161 ccgagcgcag atcctgtcac tggcacaaac gcggctgcgg acgcggccgg atctcgatcg
     2221 actcgccgaa gccgtggtcg acggccgcga cgatccgtat tccgcggccg aacggctgct
     2281 atcgtccaca cctcaaccag aggattgacg gggcgaccgc acgtcgacgt cgattttgag
     2341 cgcctcgggt tgtttcagcc acacgcctgg gttgattcgg gcgggtcgtt atcgcctcga
     2401 cgcgacactc gatcgacggt gatcagatca tttgaagcgg gtccggcggc gtcccgccag
     2461 acctgcagga tgtcgtcacg gaatcggttg gcggcgcggg tgggaatctc ggcgtcgtcg
     2521 gtgacgtggc gggcgtagtc gttgagggtg atcgcgtcgc tgatcgtcgc tccattgctg
     2581 acgaggaact ccagcaggag cggaccggct tcgaggggca ccccgccgcg gcgaaaggcc
     2641 tgaaaagctg agatcgcctt ggatttggtt ggttcgcggc cgaaggcgtc ggacgcaaac
     2701 ccacgcacca gcagactcag gaggtagcgc aatccgtact cgaccgcggg ttctgccagc
     2761 acgccgttca gcggcgccag cgcgttgcga tccttgatgt gcgttgcgcc atgcttgcgc
     2821 agccacccgg tgatcgttgg cccatcgttg cccaacacga ccagcacccg gctcgtcgtg
     2881 ttgaggtaga aagccgccgc gtccagcatg gcttcatcgg gcgacagcaa cgccaacgac
     2941 gacccccggg tcaggtcacc ccgcaagtcg gttaccaggt caacggccgg cggtagcgcc
     3001 caggcaacgc gcatggagcg cagatcggtg ccggggggcg cgacaaccgt cgcagtcccc
     3061 ggctcgctgt ccagccaagt ccgcaacacc gacgccaggt ctgtacccgt accgacaagg
     3121 tcggtacggt gcaggtactc cacgccagca gccatcggcc acccctcgaa accgatgccc
     3181 gccatagact tcattgggcc aagacaatat caccatattg cctgacgcaa tacagcgata
     3241 ttgtaggaga tctatctaag tcggcatgcg ggtggccgtt gtgctcgtca cgccactccg
     3301 agtgaggcgg tcagccgcgg agttgacccc gagagatgag ttgctccacc gctttctccc
     3361 agcgctgcag ctgtgttcgc gtggtgaatg gttcgggcag gtggcggtcg aggtggcggc
     3421 gcagaatgat cgcacccaac gtcaaaatca gcgggttcag cgccgcccac acaacgtcga
     3481 tgtcggcgtc ggtggcaccg ctgtcggcga gttgctgcca ttgtcccagc ccgatggcca
     3541 ccaaggcgtc gaaaacc
//
LOCUS       DASSGQ010000224        12679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_532295,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000224 DASSGQ010000000
VERSION     DASSGQ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12679
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532295"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1034)
                     /locus_tag="VFI55_09690"
     CDS             complement(132..1034)
                     /locus_tag="VFI55_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7074334.1"
                     /translation="MSVIAQAFRQALDAATDRLANPARVSDPDKLRGAVSGKTVLVTG
                     ASYGIGEATARKMAAAGATVLVVARSADRLDDLVAAINAGGGHSVAYPTDLSDEAAVS
                     VLTKQVTENHGPLDVVVSNAGKSLRRSLHHQYDRPHDFQRTIDINYLGPIWLLLGLLP
                     AMRERGGGHVVNVSSVGVRVVPGPQWGAYQASKGAFDRWLRSVAPELHADGVDVTSVY
                     FALVRTRMIAPTPLLGRLPGLSADEAADAIAKAVIERPRTSAPPWVLPAELASVLLAG
                     PADRAARLWHRRFFAKSDAHEARR"
     gene            1306..2049
                     /locus_tag="VFI55_09695"
     CDS             1306..2049
                     /locus_tag="VFI55_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HET7074335.1"
                     /translation="MIARQLSRFLSPAVVAACGFSASALAGVAAPPASAQPCPDVQVV
                     FARGTGEPPGPGPTGQAFADTLRSRVGAKSFDVYPVNYPASNDWDTGLDGIRDAGAHV
                     VSMAHDCPNTKMVLGGYSQGAAIMGFVTSAAVPDGIDPATVPKPMDPDVANHVSSIVL
                     FGMPNVRAMNFLNEPPVAIGPLYQAKTIKVCAPEDPVCSDGMNFAAHDTYADDGAMID
                     KGAAFAVSHLVAGPGAPVTVASPGGRFGS"
     gene            complement(2082..3458)
                     /locus_tag="VFI55_09700"
     CDS             complement(2082..3458)
                     /locus_tag="VFI55_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005082413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7074336.1"
                     /translation="MVLIDALGADGEYRTRNREVILTTGGVPVAELSIVPPLYVSRTI
                     AAQRKVRPLPAARREAALADAADVFATAVIGGLDFADYVRLASRISGLPIAVTRAGAR
                     GVADAVATAFDAVRSARPAGAAVDWREERTRHGSAVWARRGEVFAVHAAGNGPGVHGL
                     WPQALALGYRVAVRPSRREPLTAHRLVNALRQSGFRAEDAVCLPTDHAGADEIIRSAD
                     LAMVYGGQDVVDKYADDPTVVVNGPGRAKIVITADRDWRDYVDLVVDSIANLGGMACV
                     NTTAVLYEGDPAPLARAIAERLATIQPFPTDDERAILPSQPLDKAQTLANYLAVKAAG
                     TTPLLGADQVVAPLGDSYAALRPAVHLLTEPGSGALTDRLNVELPFPCVWVSPWSRDA
                     GIETLRHSLVVTAITGDEELIDDLLAEPTITNVYSGHHPTYYAAPEIPHDGFLADALM
                     RNKGFIRD"
     gene            complement(3494..4609)
                     /locus_tag="VFI55_09705"
     CDS             complement(3494..4609)
                     /locus_tag="VFI55_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016888702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenazine antibiotic biosynthesis protein"
                     /protein_id="HET7074337.1"
                     /translation="MAFADSSVDFSLLDVPRSVPIDDPEAYLRAAMSWHFGKDTGSAF
                     WLQTARTLDFNPLTDVKNFTDLRLFPNLVNELRRVPVEDLIPRGYASEGSRPPVPLIF
                     ESGGTTGAPKRTVQMPDWVAQVIQWQIQDFATGGFVGGQGFLCLMPSGPHGVGYFSRL
                     VAERLGSVFHGIDIDPRWVKKVAARNAAAEVAAYVDHVIDQAVFILQTQRVANLHTTP
                     PLLEAIARNDHVVDLVNDKIRYLLLSGAHVDVDTLDLLRDIFPNTTITMAFGSTMVLS
                     QAVTRTADGDAFVFDPRTPYVVFWVIDPDTGDRVPYGQQGQVVMNHISRGMFIPNNLE
                     RDLAIRMPGPAGELSDSVSAVRPVATFEGEAVIEGVY"
     gene            4882..6207
                     /locus_tag="VFI55_09710"
     CDS             4882..6207
                     /locus_tag="VFI55_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7074338.1"
                     /translation="MDIAYQVIGDGPVDVLVLPGPLIPIDCVDLEPSMYRFHRRLASF
                     CRVIRFDQRGIGLSSRVPSLDVMGPESWARDAVAVMDAVGCERATVFAPGFTSLAGLV
                     LAADYPERVNSLVIVNGGARTLRGPDYPIGAELSAADPFTTVGIEPDAVEQGFDVLGI
                     IAPSVAKDEAFRAWWDHAGNRAASPSMARAFIRKIREGDVRDRLERITVPTLVLHRDN
                     PNFSPVEHAHYLAQQITGARLVELPGEDALYWVGDTAPMLDEIEEFITGVRGVSDVER
                     LLTTIVFTDIVGSTQRAALLGDDRWRDLLDNHDSIVRHELQRFGGREVNTAGDGFVAT
                     FTSPSAALGCADEIVDAVRVLGIEVRVGIHAGEVEVRGDDVAGMAVHIGARVAALAAS
                     GEVLVSSTVRDIVTGSRRRFADRGESELKGVPGQWQLYALVREHATVRR"
     gene            6359..7105
                     /locus_tag="VFI55_09715"
     CDS             6359..7105
                     /locus_tag="VFI55_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7074339.1"
                     /translation="MTDVHISLSPALRRALDLLADPPAEPNVSRGYLDLLDNETAGDE
                     AVPKNTGPIQAAWASPIGSMLYDNAQAFSRRLISAWQVPVEWLAIPAGGVALDVGSGP
                     GNITASLARAAGPDAVALGVDISEPMLDRAVRNEAGPRVGFIKADAQRLPLRDKTVDA
                     VVSTAALQLVPNPTAALAEMVRVLRPGGRLAIMVPTVGPAARFWQRLPNVGAYVFDDD
                     EIGDILEANGFVSVRVKNYGPLQWVRGKNG"
     gene            complement(7120..7926)
                     /locus_tag="VFI55_09720"
     CDS             complement(7120..7926)
                     /locus_tag="VFI55_09720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7074340.1"
                     /translation="MALPPPGNDRAAIVTGASSGIGEQFARILSLRGYQVVLVARSAD
                     RLAALAGRLGPQAHPLPADLSDRTARAGLLDRVAALGLVPDILINNAGFSTLGLVAKS
                     VPEQEVNLVEVDVAAVVDLCSRFLPGMVDRRRGAVLNVASVVAFAPLPGQAAYGGAKA
                     FVLSYTHSLRGELRGTGVSATALCPGPVETGFADAAGLSKEEAESALPRVMWIPADKV
                     AEAGIDGLAAGKAVVIPGRVNRVASALARVAPPELLLPVLARNHPALKRD"
     gene            complement(7987..8451)
                     /locus_tag="VFI55_09725"
     CDS             complement(7987..8451)
                     /locus_tag="VFI55_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019929785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7074341.1"
                     /translation="MQPESEERVVSATRVITASAARIFELIADPALQPSWDGNNNLAS
                     SAPGQRVQRVGDVFKTTLTSGAVRENHVVEFIEGSKIAWRPAEPKRQPPGHLWRWELN
                     PINATLTSVTHTYDWTALTDSTRLAKARSTTPDMLRESMDRLAMIAQQPAGG"
     gene            complement(8484..9176)
                     /locus_tag="VFI55_09730"
     CDS             complement(8484..9176)
                     /locus_tag="VFI55_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7074342.1"
                     /translation="MVNIKGSTVVVTGGQRGLGKAIVDELLRRGAAKVYATARVPRPS
                     DDSRVVSVELDVTKPDSVAALASTASDADVVINNAGVLGAPKLLSSDIEEVRAVFETN
                     YFGALRVAKAFAPILADNGGGALVDIHSVLSWVGGFGGYGDSKAAFWSATNSLRIELE
                     KQGTLVTGVHLGYTDTDMVSAFDVPKNDARQVARDIVDGIERGDAEVLADDVTRSIKA
                     ALSGPVEALRVG"
     gene            9263..9883
                     /locus_tag="VFI55_09735"
     CDS             9263..9883
                     /locus_tag="VFI55_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HET7074343.1"
                     /translation="MSWVWGASQRYGLAAAPGGLGLVQDFLNTAAIDGYGSDLLADAA
                     RAGDWASGAVRTWSALRGLDVAAPPLSDADASRLRALRDTLARLVSGEPPAERGTSAV
                     PATFSLSQTGEVRLEPAGTGWRWVASALWGEVLLSQQDGTWRRIKRCHNHECASTFYD
                     RSKNNSGVWHDVKTCGNAANLRASRARRRERDRAAQKLPDTEPFGA"
     gene            complement(9921..10085)
                     /locus_tag="VFI55_09740"
     CDS             complement(9921..10085)
                     /locus_tag="VFI55_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074344.1"
                     /translation="MPPLQAWISQNPTNLDVVAPLKARACDALQAVLKSTPRRVGADS
                     RIMRKGTERC"
     gene            complement(10289..11053)
                     /locus_tag="VFI55_09745"
     CDS             complement(10289..11053)
                     /locus_tag="VFI55_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HET7074345.1"
                     /translation="MGKDRGINRWELVTCGFAGHITYAPDEKALADRLSGSTGLGDVW
                     RCLRCGDFAVGDPHGRGPAEDAPMVMRGKALRQAIIIRALALERLFRAVVIGLAAYAV
                     WKFRGAQGAIQATLDRDLPIFRAAGFKVDQMTLVHELEKAVAAKPSTLALLTAMLAGY
                     AVIEVIEGVGLWLLKRWGEYFAVVATSVFLPLEIYDLTKGVTMTRAVTFTINIAAVIY
                     LVVSKRLFGVRGGRKAYDEERRGEQLLDVERAAATA"
     gene            complement(11112..12500)
                     /locus_tag="VFI55_09750"
     CDS             complement(11112..12500)
                     /locus_tag="VFI55_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HET7074346.1"
                     /translation="MDDLAGFVVDAEPADLSGRPRALMKRNVLDSVACAVGSLDGELI
                     TTIREHTEQFSGVPTATLVGGGRASVDQAAFFNAVLVRYPDLLDTYLTVGGLCHPADN
                     FGAVLAVAEHVDAGGADFLLALAVAYEVQCRFSAQVPVMARGLNHALQLAMSAAAGSA
                     KLLGLDAEHTANAIAAATVDNVSLAAVHAEPVSNWKGISPAITGMRAVYTTMLAARGV
                     TGPKSLFEGPHGLVQLFDQPIDLHTADRGLTCVEQTYLKQYCSLIHGQVIIDAVLAIR
                     GDNDLRGADVARVTLEVFQGAYDFAGGGAYGAKDHPRTKDQADYNLKFLTAVALLDGG
                     VGPEQLEAERVSRGDVQDLLARVDVTAAADLTAGYPQRTAARVHVVTRDGREFSREQD
                     DYEGSPTRPMSWERVVDKFHWLAEPFCGEALRADIIAAVDHLDEIPVAELAGLLGAVS
                     SVPQRPRRRPRF"
BASE COUNT         2028 a         4279 c         4262 g         2110 t
ORIGIN      
        1 attcgtgccg ccccggcacg cctcgcgcgc cagccgtacg cccgcgaccg gggacaccgg
       61 accgagcaat ccggagcgca gcagcgcccg gaccgtggtg gcaaccacgc cgtcctcaga
      121 taccggcgcg atcaccgtcg ggcctcgtgg gcatcggact tggcgaagaa gcggcggtgc
      181 cagagccggg ctgcccgatc ggcgggtccc gcgagcagca ccgaggccaa ctcggccggc
      241 aacacccacg gcggcgcgct ggtgcggggc cgttcgatga cggccttggc gatcgcgtcg
      301 gcggcttcat ccgcagacag cccgggaagg cggcccagca gcggcgtggg tgcgatcatc
      361 cgggtgcgga ccagcgcgaa gtagaccgac gtcacgtcca cgccgtcggc gtgcagttcc
      421 ggtgccacgc tgcgcagcca gcgatcgaag gcacccttgg aggcctggta ggcgccccat
      481 tgcggaccgg gcacgacacg cacgccgacg ctcgaaacgt tgacgacgtg gccgccgccg
      541 cgctcgcgca tcgccggcag cagtcccagc agcagccaga tcggccccag gtagttgatg
      601 tcgatggtgc gctgaaagtc atgcggtcgg tcgtactggt gatgcaggga gcggcgtaac
      661 gacttgccgg cattgctcac gaccacgtcg agcgggccgt ggttctcggt gacctgcttc
      721 gtcagcacgc tgacggcggc ctcgtcggag aggtcggtcg ggtaggcgac agagtgaccg
      781 ccgccggcgt tgatcgccgc caccaggtcg tcgagccggt ccgctgatcg ggccaccacg
      841 agcacggtcg ctcccgcggc ggccattttg cgcgccgtcg cctcgccgat gccgtaggag
      901 gcgccggtga ccaggacggt cttgccggaa acggcgccgc gcagcttgtc gggatccgac
      961 actcgcgccg ggttggccaa tcggtcggtg gccgcgtcaa gggcttgcct gaaggcctgt
     1021 gctatcacgc tcacgtccgc tgccgtattc gctggtactg cacgtcgagg gtgcggttct
     1081 gactccaccg gtgcctcgac tcgggttacg gctagcagac cacatcggag tccaggcccg
     1141 cgcgccagcc cgccgttgcc ggctcgttcc ggaccgttcc cacagggctc gcagatatgg
     1201 cttttccatg cggcgtgtgt cgccggcgcg cgagctttac taatctgtaa taatcgccaa
     1261 agaactcact gtttaggtaa acgtatgcat gggcaccatt tcggcatgat cgcacgccag
     1321 ctctcacgat tccttagtcc cgcggtagtc gcggcatgcg ggttcagcgc ctcggcgctc
     1381 gccggcgtcg ccgctccccc cgcctctgct cagccgtgtc ctgacgtgca ggtggtattc
     1441 gcacgtggca ccggcgagcc cccgggtccc ggcccgaccg ggcaggcatt cgcagacacg
     1501 ctgcgctcgc gcgtgggcgc gaagtcgttc gacgtgtacc cggtcaacta ccccgccagc
     1561 aacgattggg ataccggtct cgacggtatt agagacgcgg gcgcccacgt cgtgtcgatg
     1621 gctcacgact gccccaacac caagatggtg ctcggcggct actcacaggg cgcggccatc
     1681 atgggcttcg tcacctcggc cgccgtgccg gacgggatcg acccggcgac ggtaccgaag
     1741 ccgatggacc ccgacgtggc taaccacgtg tcctcaatcg tgctcttcgg aatgcctaac
     1801 gtccgggcca tgaacttcct caacgagccg ccggtcgcca tcggcccgct gtatcaggcc
     1861 aagaccatca aggtgtgcgc ccccgaggac ccggtctgct ctgacgggat gaacttcgcc
     1921 gcgcacgaca cctacgcgga cgacggcgcg atgatcgaca agggcgcggc gtttgcggtc
     1981 agccacctgg tcgcagggcc cggcgcgccg gtgacggtgg cgtccccggg cggacggttc
     2041 ggcagctgac ctgaccggcc cggggctgcg ttccctcgcc gctagtccct gatgaagccc
     2101 ttgttgcgca tcagggcgtc cgccaggaac ccgtcgtggg ggatttcggg cgccgcgtaa
     2161 taggtcgggt ggtggccgct gtacacgttg gtgatggtcg gctcggccag caggtcgtcg
     2221 atcagctcct cgtcgccggt gatcgcggtg acaaccagcg aatgccgcag cgtctcgatg
     2281 ccggcgtccc gcgaccacgg cgacacccac acgcacggaa acggcagctc gacgttgagc
     2341 cggtcagtga gcgcgccgga gcccggctcg gtcagcaggt gcactgccgg acgcagtgct
     2401 gcgtagctgt cacccaaggg agcgaccacc tggtcggcgc ccagcagcgg cgtcgttccc
     2461 gcggccttga ccgccagata gttggccagc gtctgcgcct tgtcgagcgg ctggctgggc
     2521 aggatcgcgc gctcgtcgtc ggtcgggaac ggctggatgg ttgccagccg ctcggcaatc
     2581 gcccgggcca atggggcggg atcgccctca tagagcaccg cggtagtgtt gacgcacgcc
     2641 atcccgccga ggttggcgat cgagtcgacg acgaggtcga cgtagtcacg ccagtcgcgg
     2701 tcggcggtga tcacgatctt ggcccgtccc ggtccgttca ccaccaccgt cggatcgtcg
     2761 gcgtatttgt ccaccacgtc ctgaccgccg tacaccatgg ccaggtcggc cgaccggatg
     2821 atctcgtcgg ccccggcatg gtcggtgggc aaacaaaccg cgtcttcggc gcgaaatccc
     2881 gactggcgca gagcgttcac cagccggtgc gcggtcagcg gctcgcggcg cgacgggcgc
     2941 accgcaacgc ggtagcccag cgcgagcgcc tgcggccaca ggccgtgcac gccgggcccg
     3001 tttccggctg cgtgcacggc gaacacctct ccgcgccgcg cccacaccgc gctgccgtgg
     3061 cgggtgcgct cctcgcgcca atccaccgcg gcgcccgcgg gccgggccga tcgcaccgca
     3121 tcgaacgcgg tggccacggc gtcggccaca ccgcgtgccc ccgcgcgggt gaccgcgatc
     3181 ggcagccccg aaatccggct ggccagccga acgtagtccg cgaagtccag tccgccgatg
     3241 accgcggtgg cgaagacgtc ggccgcatcg gccagcgccg cttcgcgccg cgcggccggc
     3301 agcggacgga ccttgcgctg cgcggcgatg gtgcgcgaaa catacagcgg cggaacaata
     3361 ctcagctcag cgaccggaac gccgccggtg gtcaagatga cttcgcggtt gcgggtgcgg
     3421 tactcgccgt ccgcgccgag cgcgtcgatc agcaccaaat ctgttgtaac actgggctgt
     3481 tcgtgcccca cgttcagtac acgccttcga tgacggcttc gccttcgaac gtggccaccg
     3541 gccgcaccgc actaaccgaa tcgctgagtt cccccgccgg tccgggcatc cggatcgcca
     3601 ggtcgcgctc caggttgttc ggtatgaaca tgcccctgct gatgtggttc atcaccacct
     3661 ggccttgctg cccgtacggc actcggtctc cggtgtcggg gtctattacc cagaacacca
     3721 cgtagggtgt gcgcgggtcg aacacgaacg cgtcgccgtc ggcggttctg gtgactgcct
     3781 gcgaaagcac catggtgctg ccgaaggcca tcgtgatcgt cgtgttcggg aagatgtcgc
     3841 gcagcaggtc gagcgtgtcc acgtccacgt gcgcgccgct gagcaacagg tagcggatct
     3901 tgtcgttcac caagtccacc acgtggtcgt tgcgggcgat ggcctccagc agtggcgggg
     3961 tggtgtgcag attggcgaca cgctgcgtct ggagaatgaa caccgcctgg tcgattacgt
     4021 ggtcgacgta agccgcaacc tcggcggccg cgttgcgggc cgcgaccttc ttgacccagc
     4081 ggggatcgat gtcgatcccg tggaacaccg agccgagccg ttcggcgacc agacgggaga
     4141 agtagcccac accgtgcggg ccgctcggca tcaggcacag gaagccctgg cctccgacaa
     4201 agccacccgt ggcgaagtcc tggatttgcc attggatcac ctgggcaacc caatccggca
     4261 tctgcacagt ccgtttcggc gcccccgtgg taccgccgga ttcgaatatc aggggcaccg
     4321 gcggccggga tccctcagat gcatagccgc gcgggatgag atcctccacc ggcacgcggc
     4381 gcagctcgtt gacgagattg gggaacaagc gcagatcggt gaagtttttg acgtcggtca
     4441 acggattgaa gtccagcgtc cgggcggtct gcagccagaa cgcggagccg gtgtccttgc
     4501 cgaaatgcca actcatcgcg gcgcgcagat acgcctcggg atcgtcgatc ggaaccgacc
     4561 tcggaacgtc cagcagcgaa aagtcgaccg aggaatcagc gaaggccata ccatcgatcc
     4621 tgtctcatcg gcgagcggac caacaaccgt cgttgacggc cgccgggcat acagcaactg
     4681 aacgttgcct ccccggacgg cgaaatgttt gcggccatgc ggttttcagc tgtggggccg
     4741 cacctcttgg cccggcgggc gcccaggtcc cgatcatcgg ccgctagcgc ggtttcgggt
     4801 tgtcggggac ttgtcggggg cctgcattaa cctgtacccg tggtgccggt tccgcggacc
     4861 cggtacgcca gttgcggcga gatggacatc gcttatcagg tcatcggcga tggtccggtc
     4921 gacgtgcttg tgctgcccgg gccattgatt cccatcgact gcgtcgacct cgagccgtcg
     4981 atgtaccgct ttcatcgacg gctggcttcg ttctgccggg tgattcgctt cgaccagcgt
     5041 ggaatcggct tgtcgtcgcg cgtcccctcg ctggacgtga tggggccaga gtcctgggcg
     5101 cgtgacgccg tcgccgtcat ggacgcggtc ggatgtgagc gagcgacggt cttcgcgccg
     5161 ggtttcacgt cgctggccgg actcgttctc gccgccgatt accccgagcg ggtgaacagc
     5221 ctggtgatcg tcaacggcgg ggcacgcaca ctacgtggtc ccgactatcc gatcggggcg
     5281 gagctgagcg ccgccgatcc gttcacgacc gtcgggatcg agccggacgc cgtcgagcag
     5341 ggtttcgacg tgctcggcat catcgccccc tcggtggcaa aggacgaggc gttccgcgcg
     5401 tggtgggatc acgcgggcaa ccgcgccgcg tctcccagca tggcgcgtgc cttcatcagg
     5461 aagatccgcg agggcgacgt gcgcgaccgg ctcgaacgca tcaccgtgcc gacgctggtc
     5521 ctgcaccgcg acaacccgaa cttcagtcct gtcgagcatg cccattacct tgcccagcaa
     5581 atcaccggag cacgcctggt cgagctaccg ggcgaagacg cgttgtactg ggtcggcgat
     5641 accgcgccga tgctcgacga gatcgaggag ttcatcaccg gcgtgcgcgg cgtctccgac
     5701 gtcgagcgcc tgctcaccac gatcgtgttc accgacatcg tggggtccac gcagcgcgcc
     5761 gccctgctcg gcgacgaccg gtggcgcgac ctgttggaca accacgacag catcgtgcgc
     5821 cacgagctgc agcggttcgg cgggcgcgag gtcaacaccg ccggtgacgg gttcgtcgcg
     5881 acattcacca gccccagcgc cgcgcttggc tgcgccgacg agatcgtgga cgcggtgcgg
     5941 gtgctcggca tcgaggtgcg ggtgggcatt cacgcgggtg aggtcgaggt gcgcggcgat
     6001 gacgtcgcgg gcatggccgt gcacatcggc gcgcgggtgg ccgcgctggc cgcctcgggc
     6061 gaggtgctgg tgtcctcgac ggtgcgcgac atcgtcaccg ggtcgcggcg caggttcgcc
     6121 gaccgcggcg agagcgagct caaaggcgtg ccgggtcagt ggcagctgta cgcgttggta
     6181 cgcgagcacg ccaccgtccg gcggtaatcg atcgcacatc gcgcgagtcg aggagccgca
     6241 cggctcggcg taggctacga aacggggctt gtcgaagcca ccctcagttg gcttccgatc
     6301 cgattttcgg cctacggctt ggcatttgat cgcttggtat ttgatcgaag gagtacgcgt
     6361 gaccgacgta cacatctcgc tgtctcccgc gttacgccga gcgctggatc tactcgccga
     6421 tccgcctgca gagccgaacg tgagcagggg ttacctcgat ctgctggaca acgaaacggc
     6481 cggggacgaa gccgtcccga agaacaccgg cccgatccag gcggcgtggg cgtcaccgat
     6541 cgggtcgatg ctctacgaca acgcccaagc cttctcgcgc cggctgatca gcgcatggca
     6601 ggtgcccgtc gagtggttgg ccatcccggc aggcggtgta gcactggacg tcggctcggg
     6661 tccgggcaac atcaccgcgt cgctggcgcg cgccgccggc ccggacgccg tggcgctggg
     6721 cgtcgatatt tccgagccga tgctggatcg cgccgtccgc aacgaggcgg gaccccgagt
     6781 gggcttcatc aaggctgacg cacaacggct tccgctgcgc gacaagaccg ttgatgcggt
     6841 ggtctcgacc gccgcgctgc agctggttcc gaatccgacg gccgccctcg ccgagatggt
     6901 gcgggtgctg cgaccgggcg gaaggctggc catcatggtt cccaccgtcg ggccggcggc
     6961 ccggttttgg cagaggctgc cgaatgtcgg ggcgtacgtg ttcgacgacg acgaaatcgg
     7021 cgacatcctg gaagccaacg gattcgtcag cgtccgggtc aagaactacg gcccgctcca
     7081 gtgggtccgc ggcaaaaacg gctgaacctc acgcacgttt cagtcccgtt ttagcgccgg
     7141 gtggttgcgc gccagcaccg gcagcagcag ctcgggcggc gcgacccggg ccagcgccga
     7201 cgcgacacgg ttgacacgcc ccgggatgac gacggccttg cccgccgcca gaccgtcgat
     7261 tccggcctct gccaccttgt cggcgggaat ccacatcacc cgtggcaagg cggattctgc
     7321 ttcctccttg gaaagtccgg ccgcatcggc gaatccggtt tccaccggcc ccggacacag
     7381 cgcggtcgcg ctgacccccg tgccgcgcag ctcaccgcgc aagctgtggg tgtaggacaa
     7441 tacgaacgcc ttggcgccac cgtatgccgc ctggcccggc agcggggcga atgcgaccac
     7501 cgaggccacg ttcagcaccg cgcctcgacg gcggtcgacc atccccggca ggaacctgct
     7561 gcacagatcc accaccgcgg ccacgtcgac ctcgaccagg ttgacttctt gctccggaac
     7621 ggatttcgcc accagcccga gggtcgagaa accggcgttg ttgatcagga tgtccggaac
     7681 cagacccagc gccgcgacgc ggtccagcag acccgctcgc gcggtccggt cggacaggtc
     7741 ggccggcaac gggtgagcct gcggtcccag ccgtccggcg agggctgcga gacggtcggc
     7801 gctgcgggcc accagcacca cctgatagcc gcgcagcgag agaatcctcg cgaactgttc
     7861 gccgatgcct gacgacgcgc cggtgacgat ggcggcgcgg tcgttacctg gtggtggaag
     7921 ggccatacgg tgacggtagc cggtggcgcg gcgttcgcct acgcggccac cctcggttgg
     7981 tttgcgctac cccccggccg gctgttgggc gatcatggca agccggtcca tcgactcgcg
     8041 gagcatgtcg ggcgtcgtcg aacgagcttt ggccagccgg gtgctgtccg tcagcgccgt
     8101 ccagtcgtag gtgtgggtca cgctggtgag cgtggcgttg atggggttga gctcccagcg
     8161 ccagaggtgg ccgggcggct gccttttggg ttccgccgga cgccaggcga tcttgctgcc
     8221 ctcgatgaac tcgacgacgt ggttctcccg gacagcccca ctggtcagcg tcgttttgaa
     8281 cacgtcaccg acccgctgga cgcgctgccc gggagccgac gaggccaggt tgttgttgcc
     8341 gtcccagctc ggctgcaggg cagggtcggc gatcagttcg aaaatgcgcg cggcgctggc
     8401 cgtgatcacg cgggtggcgc tcaccacgcg ttcctcggat tcgggttgca tggtgctatc
     8461 caaacacgcc gcccggggca ggcctacccc actcggagcg cttcgacggg ccccgacaga
     8521 gccgccttga tggagcgggt gacatcgtcg gccagcacct cggcatctcc tcgctcgatg
     8581 ccgtcgacga tgtcccgtgc cacctggcgc gcgtcgttct tgggcacgtc gaaggcagag
     8641 accatatcgg tgtcggtgta gccgagatgc accccagtga ccagggtgcc ctgcttctcg
     8701 agctcgatcc gcagcgagtt cgtcgccgac cagaaagccg ctttggagtc gccgtaaccg
     8761 ccgaagccgc ccacccagga caggacggaa tggatgtcga ccagggctcc gccaccgttg
     8821 tcggccagga tcggcgcgaa cgccttggca acccgcagcg caccgaagta attcgtctcg
     8881 aagacggccc gcacttcctc gatgtcgctg ctgagcaatt tcggcgcacc gaggacacct
     8941 gcgttgttga tcacgacgtc cgcatcggac gccgtgctag ccagcgcggc aacggaatct
     9001 ggtttggtga cgtccagctc gacactcacc acgcgagagt catcgctggg ccgcggaacc
     9061 ctcgccgtcg cgtatacctt ggccgcgcct cgccgcaaca gctcgtccac gatggccttg
     9121 ccgagtccac gctgcccgcc ggtaaccacg accgtcgacc ctttgatgtt gaccattgat
     9181 cagtactcct tgacttaagt gaagctcctt tgcatctgtc accgtagttg actgtcgcga
     9241 catatgcaag tatattttgg ctatgtcttg ggtatggggt gcgtcacagc gctacgggct
     9301 cgctgcggcc cccgggggcc tggggctggt tcaggacttc ctcaacactg cggctatcga
     9361 cggatacgga tcggacctgc tggcagacgc cgcgcgcgcg ggtgactggg cgtccggcgc
     9421 ggtccggacc tggtcggcct tgcgggggct ggacgtggcg gcgccgccgc tgagcgacgc
     9481 ggatgcgtcg aggcttcgcg ccttgcgcga caccctcgcg aggctagtct ccggagagcc
     9541 gccggccgag cgcgggacta gtgccgttcc cgcgaccttt tcgctgtcac agaccggcga
     9601 ggtacggctg gaacccgcag gcaccggctg gcgttgggtg gcgtcggcgc tctgggggga
     9661 ggttttgctg agccagcagg acggcacctg gcggcggatc aagcgatgcc acaaccacga
     9721 atgcgcgtcg acgttctatg accgctcgaa gaacaacagc ggcgtctggc acgacgtcaa
     9781 gacctgcggc aacgcggcga atttgcgcgc ttcgcgggcc cggcgacgcg aacgcgaccg
     9841 cgcggcgcaa aagttgccgg acaccgaacc cttcggcgcg taggcgccgg atcactcagg
     9901 tgacgccgcc atggggcgca tcagcagcgc tcggtgccct tacgcatgat gcgggagtcg
     9961 gcgcccaccc ggcgcggggt gctcttcagc accgcctgca acgcgtcgca cgcgcgcgcc
    10021 ttgagcggcg cgacgacgtc gagattggtt gggttctggc tgatccaggc ttggagtggt
    10081 ggcacagttt tcatcctgtc tgttcggcca gtgagccgct tcagtaaatt tcaccgcggt
    10141 tgttttacga gcgcgccgcg ccgcggtaaa cgtcacgaaa aggcctcttg tggtgtaggg
    10201 cacatccccg tcgcaaagtg gagctcgtca caccggtgcg attcggtgtg gcaccggtac
    10261 accttcgaac cggccggatc gcgtggcgtc aggcggtcgc cgcggcccgt tcgacatcga
    10321 ggagctgctc accgcgtcgt tcctcgtcgt aggctttgcg gccgccccgc acaccgaaca
    10381 gccgcttcga gaccaccaag tagatcacgg cggcgatgtt gatcgtgaag gtgaccgcgc
    10441 gcgtcatcgt gacgcccttg gtcaggtcat aaatctccag cggcaggaag accgaggtgg
    10501 ccaccacggc gaagtactca ccccagcgct tcagcaacca caacccgacg ccttcgatca
    10561 cctcgatcac cgcgtagccg gccaacatcg ccgtcagcag ggccaacgtc gatggctttg
    10621 ccgccaccgc cttttccagc tcgtgcacca gcgtcatctg gtcgaccttg aatccggcgg
    10681 cacgaaagat cggcaggtcg cggtcgaggg tggcctggat ggcgccctgc gcgccccgga
    10741 acttccacac cgcatacgcc gcgagtccta tcaccaccgc ccgaaacaat cgttcgagcg
    10801 cgagggcccg gatgatgatg gcctggcgta acgccttgcc gcgcatgacc atcggcgcgt
    10861 cctcggcagg cccgcgtccg tgtgggtcac ccacggcgaa gtcgccgcag cgcaggcagc
    10921 gccaaacgtc gcccagaccg gtgctaccgc tcagccggtc ggcgagtgcc ttttcatcgg
    10981 gcgcataggt gatgtgtcct gcgaacccgc aagtgaccag ttcccaccgg ttgatgccgc
    11041 gatctttgcc catggccgat cattcactgc gggcagcaaa ccacgcaagg gggcgcgcga
    11101 cgagcctgga gtcagaaccg cggtcgcctg cgcggccgct ggggtaccga actcaccgcg
    11161 ccaaggagcc cggcgagctc ggcgacgggg atctcgtcca ggtgatcgac cgcggcgatg
    11221 atgtcggcgc ggagcgcttc gccgcagaac ggttcggcaa gccagtggaa cttgtcgacg
    11281 acgcgctccc aggacatcgg tctggtgggt gatccctcgt aatcgtcctg ttcgcgactg
    11341 aattcccgtc cgtcgcgggt caccacgtgc acacgggccg cggtgcgctg cggatagccc
    11401 gcggtcaggt ctgcggcggc agtgacgtcg acacgcgcca gcaggtcttg cacatcgccg
    11461 cgcgaaaccc gttcggcctc aagctgttcg gggcctaccc caccgtcgag cagcgcgacc
    11521 gcagtcagga atttgaggtt gtagtcggcc tgatccttgg tgcgcggatg atccttggcg
    11581 ccgtaggctc ccccgccggc gaagtcatac gcgccctgaa agacctcgag ggtgacccgg
    11641 gcgacgtccg caccgcgcag atcgttgtcg cctcgaattg ccaagacggc gtcgatgatg
    11701 acctgcccgt ggatgagtga gcagtactgc ttcaggtagg tctgctcgac gcaggtcagt
    11761 cctcggtcag cggtgtggag atcgatcggc tgatcgaaga gctgcaccaa gccgtgcggg
    11821 ccttcgaaca aggacttggg ccccgtgacg ccgcgcgcgg ccaacatggt cgtgtacacc
    11881 gcacgcatgc ctgtgatagc cggcgaaatg cccttccagt tcgacaccgg ctcagcgtgt
    11941 accgccgcga gggacacgtt gtcgacagtg gcggccgcga tggcgttggc cgtgtgctca
    12001 gcgtccagcc ccagcagctt cgccgacccc gctgccgccg acatcgccaa ttgcagcgcg
    12061 tgattgagcc cgcgcgccat gacgggaacc tgggcgctga accggcattg gacctcgtag
    12121 gcgaccgcga gggccagcag gaaatcggct ccgccggcgt cgacatgctc ggctacggca
    12181 aggacggcac caaagttgtc ggcgggatgg cacagcccac ccaccgtcag atacgtgtcg
    12241 agcagatcgg gatagcgcac cagcaccgcg ttgaaaaacg cggcttgatc gaccgacgcc
    12301 cgtccgcctc cgacgagcgt tgccgtgggg acgccgctga actgctcggt gtgttcgcga
    12361 atcgtcgtga tgagttcacc gtccagcgag ccgacggcgc aggcaacgct gtccagcacg
    12421 tttcgtttca tcagtgcacg gggccgcccg ctcaggtccg ccggctcggc gtcgacgacg
    12481 aagccggcaa gatcgtccac aacgtagttg tccgccaggg cttttcgtcc ttccgtcgat
    12541 cgcgctccaa gggagattat ctgtcgccac gaggccgagc cggtagccgt gtaggcgcgg
    12601 tcaaacggcc cgatccaccg catgccgctg cgacttggtg attgcggccg tctcgtcggt
    12661 gagtttcttc ccgatgacc
//
LOCUS       DASSGQ010000225         2714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_198096,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000225 DASSGQ010000000
VERSION     DASSGQ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198096"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1873
                     /locus_tag="VFI55_09755"
     CDS             <1..1873
                     /locus_tag="VFI55_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylate--amine ligase/circularly permuted
                     type 2 ATP-grasp protein"
                     /protein_id="HET7074347.1"
                     /translation="VEREIDASRAGTPATVLSPALGRAALWRAARSGLEGDLVDVRVP
                     ASKPASEVVTELVHSLRPQLEHSGDWDIVSELTRQVLIVGTSSARQRRALRRRGRLTD
                     VVDQLIAETASRWSSTATTVGDDPTLLFGYQPGSDPVVRVVGGSVDVSYDEAVDAVGH
                     PRQRYRPVLDTIAGLGAAALRLREGGIEQEQRADSVTFRVTGQSRAQLFPVDLVPRMV
                     AADEWAQLSVGLGQRARALDAFLRDIYSEQAIVADGIIGVHALDRAPGFRSTGRLAGN
                     TVRAHVSGTDLVCDRAGRWMVLEDNLRVPSGVGYAIVNHRLLSKHLPELQPPAAIEDA
                     DRAPQMLLETLRAAAPPHAPDEPAVALLSAGWEDSAWFEHTFLADEMDVALVQPSDLS
                     VSDGKLVRHIGSDVQRIDVVYARMDEDMLLSSTGYDGAPLRPGLLDAITHGHLTVANA
                     LANGVADDKAIYPYVPAMIEYYLGEKPTLSQVPTWICAERAQRDFVLANLGDLVVKPI
                     DGLGGSGVLIGPEASDAALEARRRELVTQPERFIAQVLVDLSTHPTFDGEGLYPHHVD
                     LRAFVHLRAGADGSVSTHIAPAALTRVASRGSRIVNSSYGGGSKDTWILTARPSGEG"
     gene            1905..>2714
                     /locus_tag="VFI55_09760"
     CDS             1905..>2714
                     /locus_tag="VFI55_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007236685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HET7074348.1"
                     /translation="MCGICGEIRWDGRTADVGAVTRMTGAMMSRGPDSQGVVAHGPVA
                     LGHRRLSIIDLSARGAQPMVDSDLGLTLVFNGCIYNYQELRRELEGAGYRFFSTADSE
                     VVIKAFHHWGLRCVDRFKGMFAFAIAHRDSGVVTLARDRLGIKPLYLASAPGRLRFAS
                     TVRALLRAGGVDTELDRHALHHYMTFHSVVPAPRTIYRGVRKLPPATVRVIQPDGSHT
                     DNVYWTPEFRRDPAKASWSVRDWEAALMDAFRTAVTRRMVADVPVGVLLSGG"
BASE COUNT          421 a          912 c          934 g          447 t
ORIGIN      
        1 cgtcgagcgc gagatcgacg cctcgcgggc cggcaccccc gcgacggtcc tgtccccggc
       61 actaggccgg gccgcgctgt ggcgcgccgc ccggtcgggg ttggagggcg acctcgtcga
      121 cgtccgcgtc ccggccagca agccggcatc cgaggtggtc accgagctgg ttcattcact
      181 gcgcccccaa ctggaacaca gcggtgactg ggacatcgtc agcgagttga cccgccaggt
      241 attgatcgtg ggcacgtcgt cggcgcggca acggcgggcg ctgcgccgcc gcggacgtct
      301 caccgacgtg gtcgatcagc tgatcgccga gacggccagc cgctggtcga gcaccgcgac
      361 gaccgttgga gatgacccga ccctgctttt cggctaccag cccggcagcg accctgtggt
      421 tcgcgtcgtc ggtggcagcg tcgacgtcag ctatgacgaa gccgtggatg ccgtcggtca
      481 cccgcggcag cggtatcggc cagtgctcga caccattgcc ggcctcgggg cggcggcgct
      541 gcggctccgg gaagggggca tcgaacagga gcagcgcgcg gacagcgtca ccttccgtgt
      601 caccggtcag agccgcgccc agctgttccc ggtcgacctg gtgccacgca tggtggccgc
      661 cgacgagtgg gcacagctgt cggtgggcct agggcaacgg gcccgggcgc tggacgcgtt
      721 cctgcgcgac atctattccg agcaggcgat cgtcgccgac ggcatcatcg gtgtgcacgc
      781 cctcgaccgg gcacccgggt tccgctcgac cgggcgcctg gccggcaata ctgtgcgtgc
      841 gcacgtcagc ggcaccgacc tggtttgcga ccgcgccggc cgttggatgg tgctcgaaga
      901 caacctgcgg gtgccgtcgg gcgtcgggta cgcgatcgtc aaccaccggc tcctgagcaa
      961 gcatctgccc gagctgcaac cgcccgccgc gatcgaggac gccgaccggg caccacagat
     1021 gctgcttgag acgctgcgtg cggcggcgcc cccacacgcc cccgacgagc ccgcggtggc
     1081 gctgctgtcc gccggctggg aagactccgc ctggttcgag cacaccttct tggctgacga
     1141 gatggacgta gcgctggtgc agccgtcgga cctatcggtg agcgatggaa agctggtgcg
     1201 ccacatcggt tctgacgtcc agcgtatcga cgtcgtctac gcgcggatgg acgaggacat
     1261 gctgctgtcc tccaccggat acgacggtgc tccgctgcgg ccagggctgc tcgacgcgat
     1321 cacccacggc catctcaccg tcgccaacgc gctcgccaac ggcgtggccg acgacaaggc
     1381 gatctacccg tacgtcccgg ccatgatcga gtactaccta ggcgaaaaac ccacgctgtc
     1441 ccaagtgccg acctggatct gcgccgagcg tgcgcaacgc gacttcgtat tggccaacct
     1501 cggcgatttg gtggtgaaac cgatcgacgg gctaggcgga agcggcgtgc tgatcgggcc
     1561 ggaagcctcc gatgccgcgc tggaggcgcg ccgacgtgag ttggtcaccc aaccagagcg
     1621 gttcatcgcc caggtactgg tcgacctgtc gacccatccc acgttcgacg gtgaagggct
     1681 gtacccgcac cacgtcgacc tgcgcgcgtt cgtccatctc cgggctggtg cggacggatc
     1741 ggtgtcgaca cacatcgcac ctgcggcgtt gacgcgagtg gccagccgcg ggtcgcggat
     1801 tgtcaactcg tcttatggcg ggggcagtaa ggacacctgg attctcaccg cccgaccctc
     1861 cggcgaaggt tagacggcaa cttcagtcaa cggaagcaga agacatgtgc ggtatctgcg
     1921 gtgagatccg ctgggatggg cggacagcgg atgtcggcgc agtcactcgg atgaccggcg
     1981 cgatgatgtc gcggggaccg gattcccaag gcgtagtggc ccacgggcca gtggctctcg
     2041 ggcatcggcg gctgtcgatc atcgacctgt ccgcgcgcgg cgcccagccg atggtcgata
     2101 gtgatctcgg gttgacgctg gtattcaacg gctgcatcta taactaccag gagctgcgcc
     2161 gggaacttga aggcgccggg taccgcttct tctcgaccgc cgacagcgag gtcgtgatca
     2221 aggcgttcca ccactggggt ctgcggtgcg tcgaccggtt caaggggatg ttcgcattcg
     2281 caatcgccca ccgcgactcg ggcgtggtga cgctggcgcg ggaccggctg ggcataaagc
     2341 cgttgtatct ggcgtcggcg cccggccggc tgcgattcgc atccaccgtg cgtgcgttgc
     2401 tgcgtgccgg cggcgtggac accgaactgg accgccacgc gctgcaccac tacatgacct
     2461 tccactctgt ggtgccggca ccccgcacga tctaccgcgg tgtgcgtaag ctgccgccgg
     2521 caacagtgcg ggtgattcag cccgacggtt cgcacaccga caacgtgtac tggacaccgg
     2581 agttccgccg cgatccggcc aaggcgtcct ggtcggtccg cgactgggag gccgcgctga
     2641 tggatgcgtt tcgtactgcc gtgacgcgcc ggatggtcgc cgacgtaccc gtcggcgtgt
     2701 tgctctccgg tggc
//
LOCUS       DASSGQ010000226        31381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_167885,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000226 DASSGQ010000000
VERSION     DASSGQ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 31381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31381
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167885"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VFI55_09765"
     CDS             <1..858
                     /locus_tag="VFI55_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HET7074349.1"
                     /translation="LAAKADVLLDCFRPGTCERLGIGPEDCAAVNPRLIFARMTGWGQ
                     VGPRAHTAGHDINYLSQTGALSALGYRDRPPMPPLNLVADFGGGSMLVLLGIVAALYE
                     RERSGIGQVVDAAMVDGVSLLAQMMWTMKSTGALRDERESFLLDGGAPFYRCYATSDG
                     KYVAVGAIEPQFFAELLSGLGLSPDEVPGQLDIGSYQRMYDVFAERFASRTRDEGTQV
                     FAGTDACVTPVLAWREAGADPHLRARATVITAHGVEQAAPAPRFSRTPTGPVGPPPAT
                     TTPLTEIDW"
     gene            913..1368
                     /locus_tag="VFI55_09770"
     CDS             913..1368
                     /locus_tag="VFI55_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7074350.1"
                     /translation="MAVKASREFVVDAPPEVVMAALRDVGLLSSWSPLHKQMEVIDRY
                     PDGRPHHVKATVRILGLVDKEVLEYHWGPDWMIWDAKRTSQQRGQHVEYNLKPEGVDK
                     TRVRFDITVEPAGPIPGFVVKRATENVLNAAVKGLSDLVVADRDAGKAK"
     gene            1419..1874
                     /locus_tag="VFI55_09775"
     CDS             1419..1874
                     /locus_tag="VFI55_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7074351.1"
                     /translation="MAVRASSEIVIDAPPEVIMEALADMPAVPSWSSVHKRVIVVDEY
                     PDGRPHHVKVTIKVTGIGDTELLEYHWGPDWMVWDAKKTAQQHGQHGEYTLIREGDEK
                     TRVRFAITVELWAPLPEFWVNRARRKILYAALEGLRQRVLGAGRSGPAA"
     gene            complement(1879..3069)
                     /locus_tag="VFI55_09780"
     CDS             complement(1879..3069)
                     /locus_tag="VFI55_09780"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7074352.1"
                     /translation="MTVSRLRPYATTVFAEMSALAARIGAVNLGQGFPDDDGPPALLK
                     AAQDAIAEGANQYPPGIGIAPLRHAIAAQRWRHFGIEYHPDTEVLVTVGATEAIAAAV
                     MGLVEPGSEVILIEPFYDSYSPVVAMACAHRVTVPLVPDGRGFALDADALRRAVTPRT
                     RALIVNSPHNPTGTVLSAVELAAIAEIAVAADLLVITDEVYEHLVFSSPEGHRHLPLA
                     GFDGMAERTITVSSAAKMFNCTGWKIGWACGPAQLIAGVRAAKQFLTYVGGAPFQPAV
                     ALALDTEDAWVAELRRSLQARRDRLAAGLTEIGFAVHDSDGSYFLCADPRPLGYDDST
                     AFCAELPEKVGVAAIPMSAFCDPAAAHAVAQAQVWNHLVRFTFCKRDDTLDEAIRRLA
                     ALRH"
     gene            3254..4465
                     /locus_tag="VFI55_09785"
     CDS             3254..4465
                     /locus_tag="VFI55_09785"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET7074353.1"
                     /translation="MSEEAFIYEAIRTPRGKQKNGALHEVKPLSLVVGLIDELRRRNP
                     DLDENLISDLILGVVSPVGDQGGDIARAAVLASGMPVTSGGVQLNRFCASGLEAVNTA
                     AQKVRSGWDDLVLAGGVESMSRVPMGSDGGAMGLDPATNYDVMFVPQGIGADLIATIE
                     GFSREDVDNYALRSQEKSAQAWSGGYFAKSVVPVRDQNGLLILDHDEHMRPDTKMEGL
                     AKLKPAFEGLAALGGFDDVALQKYHWVEKINHVHTGGNSSGIVDGAALVMIGSEKAGA
                     AINSTPRARIVATATSGADPVIMLTGPTPATRKVLDRAGLTVDDIDLFELNEAFASVV
                     LKFQKDLNIPDEKLNVNGGAIAMGHPLGATGAMILGTMVDELERRNARRALVTLCIGG
                     GMGVATIIERV"
     gene            4470..6632
                     /locus_tag="VFI55_09790"
     CDS             4470..6632
                     /locus_tag="VFI55_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET7074354.1"
                     /translation="MSDNTIQWDKDADGIVTLTMDDPSGSANVMNDAYIESMGKTVDR
                     LVAEKDSITGVVITSGKKTFFAGGDLTSMIKAGPEDAGQFFDSVETVKKQLRTLETLG
                     KPVVAAINGAALGGGLEIALACHHRIAADVKGSQLGLPEVTLGLLPGGGGVTRTVRML
                     GIQNAFVTVLAQGTRFKPAKAKEIGLVDEVLPTVEELVPAAKAWIKEELKANPDGAGV
                     QPWDKKGYKIPGGTPSSPGLAAILPSFPSNLRKQLKGAPMPAPRAILAAAVEGSQVDF
                     DTATRIESRYFTQLVTGQVAKNMIQAFFFDLQHINSGGSRPEGIGKTPINKIGVLGAG
                     MMGAGIAYVSAKAGYDVVLKDISLEAAQKGKGYSEKLEAKALERGRTSPEKSRALLDR
                     ITPTGDPSDLAGVDFVIEAVFENQDLKHKVFQEIEDVVEPNAVLGSNTSTLPITGLAT
                     GVKRQEDFIGIHFFSPVDKMPLVEIIKGEKTSDETLARVFDYTLAIGKTPIVVNDSRG
                     FFTSRVIGTFVNDALAMLGEGVEPASIEQAGSQAGYPAPPLQLSDELNLELMHKIAVA
                     GRKAVEDAGGTYQPHPAEAVVEKMIELGRSGRLKGAGFYEYVDGKRTRLWPGLREVFK
                     SGSSQPPLQDMIDRMLFAEALETQKCLDEDVLTSTVDANIGSIMGIGFPPWTGGSAQY
                     LVGYSGPAGTGKEAFVARARELADKYGERFLPPDSLVN"
     gene            complement(6643..7287)
                     /locus_tag="VFI55_09795"
     CDS             complement(6643..7287)
                     /locus_tag="VFI55_09795"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7074355.1"
                     /translation="MASRHSLFQIFYGLGFTPWDGHALSERLRELIEGTADIPALPPE
                     AALDVGCGTGDSSVYLARHGWHVTGVDFVPKALDKARAKARTAGVPVDFIHADVTHLS
                     QAGLDTPFQLIVDNGCLHGMSDDDRDLYVREITAAAAPGARLLIVAAKPGGPIGFLGL
                     DEAEVEHRFTPAWKLLSATDERLPFGEGPATRFTPVAWVRNRFVVHCYVLQRTG"
     gene            complement(7312..8523)
                     /locus_tag="VFI55_09800"
     CDS             complement(7312..8523)
                     /locus_tag="VFI55_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7074356.1"
                     /translation="MRILISGASISGPVLAYWLTRYGFEVTVVERAPALRKTGGHAVD
                     LFRPAMEISEKMGVLPKIEALATGTDVLTMYREGARRAARIDLTKVVGATSDRHVEIM
                     RDDLSEAYYDAGRDHVEYLFGDSITAISPDGEVTFEHAAPRWFDVVIGADGLHSNVRR
                     LVFGQDAGRTQFLGGYLAVLSVPKTLAREGEMVVHVGVGRLAGIYTAQPLDDARALFM
                     FRSNQELEYHYRDAQRQQELLRTAFAGMHETVDGWLDELDRTPTFYFDSITQLLLDTW
                     SRGRVTLVGDAGYCPGPAVGGSTSIAVLGAYVLAGELAQARGDYAQAFAAYEREMADP
                     VRRSRAMARGIAKSLVPNSRAGVWALTRGAQVISLLPAGLTKAVAKLNTKGVRLYDSM
                     QFKEYSSPVAG"
     gene            8603..9139
                     /locus_tag="VFI55_09805"
     CDS             8603..9139
                     /locus_tag="VFI55_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7074357.1"
                     /translation="MSPPRSDTRQRIQDVARDLFLQQGVQRTSLQDIADKLGITKPAL
                     YYHFSSREDLVRSILVPLIDEGEQFVDEQEARGHTDARELLGGYFDFHYRHREDLVLV
                     LAELTALADLGLIDKVLAWRDRLGKLVFGPRPTLAQSTRAVVAFGGLQDCCLQFPDTP
                     YQELRANSVEAALAALGK"
     gene            complement(9215..9529)
                     /locus_tag="VFI55_09810"
     CDS             complement(9215..9529)
                     /locus_tag="VFI55_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074358.1"
                     /translation="MTNERSTPPANESNSADARRRQIRVMQLLFAGAAAVATLALFSA
                     AGPSATHANTASGPAPLTAPIGMQLAQNTSGAASAINVDDDTNPSSGDIFTQNAQDQS
                     TA"
     gene            9641..10630
                     /locus_tag="VFI55_09815"
     CDS             9641..10630
                     /locus_tag="VFI55_09815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET7074359.1"
                     /translation="MRFGLFIPQGWRMDLVGIDPASQWAVMRDLATYADGSAWDSLWV
                     YDHFHTVPLPAGEATHEAWSLMAAYAATTSRIKLGQMCTAMSYRNPVYLAKVAATADI
                     ISGGRIQMGIGGGWYEHEWRAYGYGFPSAGVRLGRLDEGVQIMRDAWRDGKVSFNGKH
                     YQVDGAIVAPKPLQDNGIPLWIAGGGEKVTLRIAAKYAQYTNFTPALPEFAHKSEVLA
                     EHCREVGTDFDAIVRSANFSVIVGTSDAEINDRQQRIRGRLSGYVPDAVLDSMIAASS
                     GPDSITGTPEQVVERIGKVRELGCDYAICYFPEAAYDRSGIELFEREVIPALS"
     gene            complement(10632..10925)
                     /locus_tag="VFI55_09820"
     CDS             complement(10632..10925)
                     /locus_tag="VFI55_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET7074360.1"
                     /translation="MPVTVILELRFKPDAVSAGRDLMRRTLQVTRAFDGNSGTDVLVD
                     QDDEAHWLIYELWDTVEHDEAYRKFRAGEGKVTELPPLLAAPPVKTRYVTADV"
     gene            11285..11710
                     /locus_tag="VFI55_09825"
     CDS             11285..11710
                     /locus_tag="VFI55_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HET7074361.1"
                     /translation="MQKALAPEISTWPDENPQLIGSRCGNCRATTFPVQQWCPRCSGG
                     EMTALLLPRRGTLVAWTTQGFPPGAPYAGPTGKDFVPFGVGLVQLGLGEDAVIRVEGR
                     LTENDPAKLRFGQEVELTMVPLTTDEEGAEVMTFAFRPV"
     gene            11853..12998
                     /locus_tag="VFI55_09830"
     CDS             11853..12998
                     /locus_tag="VFI55_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET7074362.1"
                     /translation="MTNDVAIIGVGLHPFGRFDKTAMQMGAEAIQSALTDAGVDWKDI
                     QFGFGGSYEVSNPDAVTRLVGLTGITFTNVFNACATAASAIAQTADTLRLGRYDIGIA
                     IGLDKHPRGAFTDDPAKLALPQWYAQNGQFVTTKFFGMKANKYLHDHNISQSTLAKVA
                     AKNFRNGALNPNAFRRKPIPENEILNSAVLNYPLTQYMFCAPDEGAAAVVMCRGDIAH
                     KFTDKPVYVPACEIRTRRYGAYEVHATSAPLDEDASPTVYAAKAAYEAAGIGPEDVDI
                     AQLQDTDAGAEVIHMAETGLCADGEQEKLVADGATEIHGSIPVNTDGGLIANGEPIGA
                     SGLRQVHELVRQLRGEAGERQVPGNPRVGLAQVYGAPGTASATILSL"
     gene            complement(13017..14666)
                     /locus_tag="VFI55_09835"
     CDS             complement(13017..14666)
                     /locus_tag="VFI55_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase XPB"
                     /protein_id="HET7074363.1"
                     /translation="MTDGPLIVQSDKTVLLEVDHELAGAARAAIAPFAELERAPEHVH
                     TYRITPLALWNARAAGHDAEQVVDALVSFSRYAVPQPLLVDIVDTMARYGRLQLVRHP
                     AHGLTLVSFDRAVLEEVLRNKKIAPMLGARIDADTVVIHPSERGRVKQMLLKIGWPAD
                     DLAGYVNGEAHPISLHEDGWHLRDYQQLATDSFWAGGSGVVVLPCGAGKTLVGAAAMA
                     KAGATTLILVTNIVAARQWKRELIARTSLTEDEIGEYSGERKEIRPVTISTYQMITRR
                     TKGEYRHLELFESRDWGLIIYDEVHLLPAPVFRMTADLQSKRRLGLTATLIREDGREG
                     DVFSLIGPKRYDAPWKDIEAQGWIAPAECVEVRVTMTDSERMTYATAEPEERYRLCST
                     VHTKIAVVKSILQKHPGEQTLVIGAYLDQLDELGAELNAPVIQGSTRTTDREALFDAF
                     RRGEVSTLVVSKVANFSIDLPEASVAVQVSGTFGSRQEEAQRLGRLLRPKSDGGGAIF
                     YSVVARDSLDAEYAAHRQRFLAEQGYGYIIRDADDLLGPAI"
     gene            complement(14785..17034)
                     /locus_tag="VFI55_09840"
     CDS             complement(14785..17034)
                     /locus_tag="VFI55_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HET7074364.1"
                     /translation="MTDNTPDIPLGSWLADLPDDRLIRLLELRPDLAQPPPGSIAALA
                     ARAQARQSVKAATDDLDFLRLAVLDALLVLQADSAPVSTAKLLALIGDRVPEADVVGA
                     LDDLRSRALVWGEASLRVAADAASALPWHPGQVMLEDTSHTGEQIAGLIDGLDQAQLE
                     VLEKLLEGSPLGRTRDAAPGAPADRPVPQLLAMGLLRRIDAETVILPRHVGQVLRGEN
                     PGPTQLTAPDPVASKTTPDDVDAAAAGAVIDLLREIDVLLQTLGAAPVSELRSGGLGI
                     RDVKRLAKATGIGEPRLGLILEVAAAAGLIASGMPDPQPLDGDAPYWAPTVATERFAA
                     MSPAERWHLLASTWLELPARPALIGGRGPDAKPYGALTDSLYSTAAPLDRRLLLGMLS
                     ELPPGAGVDASAASAALIWRRPRWTRRLQPHPVADLLAESHAMGLAGRGAISTPGRAL
                     LDEAVAPEVAVEAMARALPAPVDHFLVQADLTVVVPGPLQRDLAEDLASVATVESAGT
                     AMVYRISEQSIRHALDIGKTRDWMHELFRRHSKTPVPQGLTYLIDDVARRHGQLRIGM
                     AASFVRCEDPALLAQAVAAAGELQLRALAPTVAVSPAPIADVLVALRAAGFAPAAEDS
                     TGSIVDVRPRGARVPAPQQRRPYRPLPRPSSETLNAVVSVLRKVTAAPFGNIRIDPAV
                     TMSLLQRAAKDRDTLMIGYLDAAGVATQRVVSPITVRGAQLVAFDSASGRLRDFAIHR
                     ITSVVSADD"
     gene            17188..17388
                     /locus_tag="VFI55_09845"
     CDS             17188..17388
                     /locus_tag="VFI55_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074365.1"
                     /translation="MADIAEDKASKTRYVDNGWPTTDPDDHAVSELVSDRTGASSPFG
                     ELVFPLPATDLPYVHPVTVVNR"
     gene            17398..17925
                     /gene="moaC"
                     /locus_tag="VFI55_09850"
     CDS             17398..17925
                     /gene="moaC"
                     /locus_tag="VFI55_09850"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HET7074366.1"
                     /translation="MARASEAAKPSAAWASGESRPGTLSHLDERGAAHMVDVTGKGAT
                     KRTAVAAGALRTTPQVVALISTGGLPKGDALATARVAAILAAKRTSDLIPLCHQLALT
                     GVDVDFVIGESDIEITATVRSTDRTGVEMEALTAVSVAGLTLYDMIKAVDPAARIDDV
                     RVLRKQGGKSGSWTR"
     gene            17913..18407
                     /locus_tag="VFI55_09855"
     CDS             17913..18407
                     /locus_tag="VFI55_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HET7074367.1"
                     /translation="MDTVTSRRSARVIIASTRAAAGVYDDQCGPIIAEWLEQRGFSAV
                     QPEVVADGQLITKALLSGVDAGVDVIITSGGTGISPTDSTPDQTVAVLDYMIPGLADA
                     IRRSGLPKVPTSVLSRGVCGVAGGTLIVNLPGSPGGVRDGLGVLADVLDHALDQIAGG
                     DHLR"
     gene            18404..18829
                     /locus_tag="VFI55_09860"
     CDS             18404..18829
                     /locus_tag="VFI55_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HET7074368.1"
                     /translation="MTRVLRAAITEEPIFLAEHEELVGHQSAGAIVGFVGMIRDHDGG
                     RRVVRLEYSAHPSAAQVIAQLVAEVAEEASGVRAVAASHRIGALHIGEAALVAAVAAD
                     HRQAAFETCARLVDTIKARLPVWKHQFFDDGTEEWVGSA"
     gene            complement(18927..19748)
                     /locus_tag="VFI55_09865"
     CDS             complement(18927..19748)
                     /locus_tag="VFI55_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HET7074369.1"
                     /translation="MSGRHRKPTTSSISVAKIAFTGAVLGGGSIAMAGQAAAATDGEW
                     DQVARCESGGNWGINTGNGYFGGVQFTASTWSSHGGGEFAPSAQLATKDQQIAVAERV
                     LADQGRGAWPVCGGPLSGPTPREVPAPTGMDAPLDLPWLNGLPAPLAPPPPDAPPPDA
                     PPPPPPPPPADPPAPVQLAGFDQAAPPADVPPAPPADLAPPAPPADAPPPDAAFPAPA
                     PADVPPPPGDVDAAPAEPQHASKVGYTRQLWDAIRDADIHGNDALDAVAQPSGIN"
     regulatory      complement(19760..19972)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(20177..20467)
                     /locus_tag="VFI55_09870"
     CDS             complement(20177..20467)
                     /locus_tag="VFI55_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET7074370.1"
                     /translation="MASPTGQTAAEAEGIQVTVRYFAAACEAAGTEWETLVVRPDTTV
                     AELVDRLAIRGSRLATVLSRCSYLRDGIAVRDETTMLRSGDTIDVLPPFAGG"
     gene            complement(20460..21563)
                     /gene="moaA"
                     /locus_tag="VFI55_09875"
     CDS             complement(20460..21563)
                     /gene="moaA"
                     /locus_tag="VFI55_09875"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HET7074371.1"
                     /translation="MTLTALGLPTVPSRMAAPADGRIVADRARHAPTAGPLLDTYGRA
                     ATDLRVSLTDRCNLRCTYCMPADGLDWLPGEQLLRPEELARLMHIAVTRLGITCVRFT
                     GGEPLLSRHLEDVVAAAAGLRPRPEISLTTNGVGLARRATALAKAGLDRVNVSLDSVD
                     RDHFAAITRRDRLTEVLHGLAAASEAGLAPVKVNAVLDAVTGREDVVELLRFCLEHGY
                     QLRVIEQMPLDAGHQWRREAALTADDVLAALRPHFRLRPDPAPRGSAPAELWVVDTGP
                     NTPSGKFGLIASVSHAFCSTCDRTRLTADGQIRSCLFSAEETDLRGLLRGGAQDDTIE
                     AAWRAAMWAKPAGHGINDPNFIQPDRPMSAIGG"
     gene            complement(21560..21964)
                     /locus_tag="VFI55_09880"
     CDS             complement(21560..21964)
                     /locus_tag="VFI55_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HET7074372.1"
                     /translation="MRLVLNVIWLLFGGLWMAAGYVLAALISFLLVITIPFGVASLRI
                     ASYALWPFGRTIVDKPTAGTGALLGNVIWVLLFGIWLAIGHLVSAVAMALTIIGIPLA
                     LANLKLIPVSLMPLGKQIVPVDSSHRFAQAPA"
     gene            22126..22533
                     /locus_tag="VFI55_09885"
     CDS             22126..22533
                     /locus_tag="VFI55_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET7074373.1"
                     /translation="MPSGKVKWYDAEKGFGFLSQEDGEDVYVRSSALPAGVEGLKAGQ
                     RVEFGVAAGRRGPQALSLKLIDPPPTLAKTRREAPVEHKHSPDELHGMVEDMITLLES
                     TVQPELRKGRYPDRKAARRVSEVVKAVARELDS"
     gene            22650..24590
                     /locus_tag="VFI55_09890"
     CDS             22650..24590
                     /locus_tag="VFI55_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7074374.1"
                     /translation="MAQQTQVTAEQARALAEESRESGWDKPSFAKELFLGRFPLELIH
                     PFPRPSDAEQARTSAFLGKLREFLDTIDGSVIERDARIPDEYVKGLAELGCFGMKIPA
                     EYGGLHMSQVAYNRALMMASSVHPSLGALLSAHQSIGVPEPLKLAGTPEQKQRFLPRC
                     AAGAITAFLLTEPDVGSDPARLASTATVIEDGAAYELEGVKLWTTNGVVAELLVVMAR
                     VPRSEGHRGGISAFVVEADSPGITVERRNKFMGLRGIENGVTRLHRVRVPRENLIGRE
                     GDGLKIALTTLNAGRLSIPAIATGSAKWSLKIAREWSGERVQWGKPLGKHEAVASKIS
                     FMAATNYALDAVLELSAQMADEGRNDIRIEAALAKLWSSEMACLIADDLIQIRGGRGY
                     ETAESLAARGERAVPAEQSWRDLRINRIFEGSSEIMRLLIAREAVDAHLTAAGDLAKP
                     DSDLRQKAAAAVGASGFYAKWLPQLVFGEGQRPLAYHEFGRLASHLRFVERSSRKLAR
                     NTFYGMARWQADLELKQGFLGRVVDIGAELFAMSAACVRAEAQRAADPVVGRQAYELA
                     ETFCEQATLRVEALFHGLWSNTDSSDVRLTDAVLEGRYTWLEDGIVDQSEGTGPWITH
                     WEPEASAETNLARRFLTVHVPS"
     gene            24702..25706
                     /locus_tag="VFI55_09895"
     CDS             24702..25706
                     /locus_tag="VFI55_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HET7074375.1"
                     /translation="MTSYVAPAGEFTRDTNYISTRITADGRDGYPVEPGRYRLVVARA
                     CPWANRAIIVRRLLGLEDVLSIGFCGPTHDERSWTFDLDPGGVDPVLKIPRLQDAYFK
                     RIPDYPKGITVPAIVDVLTGAVVTNDFAQMTLDFSTEWVAYHRDGAPQLYPEPLRAEI
                     DQVSRRIYTEINNGVYRCGFAGTQQAYEAAYDRLFTALDWVSDRLRNQRYLVGDTITE
                     ADVRLFTTLARFDPVYHGHFKCNRSKLSEMPVLWSYARDLFQTPGFGDTVDFVQIKQH
                     YYIVHADINPTGIVPKGPDLADWLGPHGQEALGGRPFGEGTPPGPPRDGERVPADHGA
                     "
     gene            complement(25724..26227)
                     /locus_tag="VFI55_09900"
     CDS             complement(25724..26227)
                     /locus_tag="VFI55_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2771 domain-containing protein"
                     /protein_id="HET7074376.1"
                     /translation="MKRGVAVLLAVVVLLLAAAAGVGAWLLARPAGPQQPEISAYSHG
                     HLTRVGPYLYCNVLDLNDCQAPQTQGELPFNGDFPVQLSVPEAISRAPWRLLQVYEDP
                     ANTTTTMFRPGTRFAVTIPSVDPQRGRLTGIVVQLLTLVVDDSQPSDTTTVRAVPHAE
                     WSVRLTF"
     gene            complement(26224..27840)
                     /locus_tag="VFI55_09905"
     CDS             complement(26224..27840)
                     /locus_tag="VFI55_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7074377.1"
                     /translation="MANYPADDPDYRRTRRPSPMPSANRYLPPLGQEPEPDRGNGAQP
                     PGPAPGERITVTRAAAQRSREMGSRMYWMVQRAATADGADKSGLTALTWPVVANFAVD
                     SAMTVALANTLFFAAASGESKSKVALYLLITIAPFAVIAPLIGPALDRLQHGRRVALA
                     LSFALRTVLAVVLIMNYDGAAGSFPSMVLYPCALAMMVFSKSFSVLRSAVTPRVMPPT
                     IDLVRVNSRLTVFGLLGGTIAGGAIAAGVEYVCTHLFKLPGALFVVVAITIGGVFLSM
                     RIPRWVEVTEGEVPATLSYHRDSEQPRRWGRLGHWPEEVKKVSGTLRQPLGRNIITSL
                     WGNCTIKVMVGFLFLYPAFVAKAHQANGWVQLGMLGVIGAAAAIGNFAGNFTSARLQL
                     GRPAVLVVRCTVAVSAVAVAASVAGNLMVAVIATLVTSGSSAISKASLDASLQHDLPE
                     ESRASGFGRSESTLQLAWVLGGALGVLVYTELWVGFTAVSALLILGLAQTLVSFRGDS
                     LIPGLGGNRPVMVEQEGLRRGAARPAAVPE"
     gene            28028..28903
                     /locus_tag="VFI55_09910"
     CDS             28028..28903
                     /locus_tag="VFI55_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HET7074378.1"
                     /translation="MTAPTEEPAATSVAESPAELTAVLTGAVGLARAAVEEFSGLEAV
                     GDHLGVSFEDPNAATHRFLAHLPGYQGWQWAVVVAAYPGTEHVTISEVVLIPGPTALL
                     APDWVPWEQRVRPGDLSPGDLLAPAADDPRLLPGYTASGDPQVDDVAAEIGLGRRWLL
                     SALGRTEAAERWHTGEYGPDSAMARSTKRVCRDCGFFLPLAGSLGAMFGVCGNELAAD
                     GRVVDKQYGCGAHSDTPAPAGTGSPIYEPYDDGVLDVVETSSESLVESADLTESQPEP
                     PESSESPPEPVNSPD"
     gene            complement(28900..29355)
                     /locus_tag="VFI55_09915"
     CDS             complement(28900..29355)
                     /locus_tag="VFI55_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7074379.1"
                     /translation="MVAPLLQTQVDINAPVSRVWALISDFRRMPAWSPQCRWMKPLGP
                     LRAGTRTVNLNRRHRLFWPTTCTVVEVIPEKKLAFRVDTNRTIWSYELEPNGQGTRLI
                     ESRHAENGVSAISSLGVNALLGGTANFERELLDGMNTSLANIKAAAEKG"
     gene            complement(29381..29656)
                     /locus_tag="VFI55_09920"
     CDS             complement(29381..29656)
                     /locus_tag="VFI55_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003404603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2530 domain-containing protein"
                     /protein_id="HET7074380.1"
                     /translation="MSEEPPESRVPPALPPALLEVWPFIAVGFLGWLICAVLAFVVPA
                     LQSWRSVTLGGLGVGLIGTSIFVWQLAAARRGARGAQSGLETYLDRK"
     gene            29869..30300
                     /locus_tag="VFI55_09925"
     CDS             29869..30300
                     /locus_tag="VFI55_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7074381.1"
                     /translation="MPDSDARLASDLSLAVMRLARQLRFRNPSSPVSLSQLSALATLA
                     NEGAMTPGALAIRERVRPPSMTRVIASLADMGLVDRAPHPVDGRQVLVSVSESGAELV
                     KANRRARQEWLAERLVTLDSEKREVLRQAADLMLALVDESP"
     gene            30297..31187
                     /locus_tag="VFI55_09930"
     CDS             30297..31187
                     /locus_tag="VFI55_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET7074382.1"
                     /translation="MSARRYSDVDVRDVDDPDDPRLDDFRDLNSVDRRPDLPTGKGLV
                     IAEGVLVVQRMLASRFTPHALLGTDRRLVELNDDLAGAKAPFYRASADVMARVVGFHL
                     NRGVLAAARRVPEPGVAEMVEGARTVAVLEGVNDHENLGSIFRNAAGLGVDAVVFGSG
                     CADPLYRRAVRVSMGHALLVPYARATNWPADLVLLKDKGFRLLAMTPHSAAGTLAEAM
                     AAARDERIAVVLGAEGPGLTEVALRLSDMRVRVPMSRGTDSLNVATAAALAFYERARL
                     GDDGARGGSPRLPGNTGKAG"
     gene            31197..>31381
                     /locus_tag="VFI55_09935"
     CDS             31197..>31381
                     /locus_tag="VFI55_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2537 domain-containing protein"
                     /protein_id="HET7074383.1"
                     /translation="MSDQSVPWATGLTVSAFVAAVTGAAVVVLTLGLIRVHPLLAVGL
                     NIVAVGGLAPTLWGWRRV"
BASE COUNT         5334 a        10122 c        10729 g         5196 t
ORIGIN      
        1 ctggcggcca aggccgacgt cctgctggac tgcttccggc ccggcacgtg cgagcgactc
       61 ggcatcggtc ccgaggactg cgcggcggtc aatccgaggc tgatcttcgc gcgaatgacc
      121 ggctggggac aggtcgggcc gcgggcccac acggcgggcc acgacatcaa ctacctgtcc
      181 cagaccggtg cgctctcggc gctgggctat cgcgatcggc cgcccatgcc gccgctgaac
      241 ctggtcgccg acttcggcgg cggttcgatg ctggtgctac tgggcatcgt cgccgcgttg
      301 tacgagcggg aacgctcggg aatcggccag gtcgtcgacg ccgcaatggt tgacggggtc
      361 agcctgctgg cccagatgat gtggaccatg aagtcgaccg gtgcgctgcg cgatgagcgc
      421 gaatcgtttc tgctcgacgg cggcgccccg ttctaccgtt gctacgcaac ctccgatggc
      481 aagtacgtgg ccgtcggcgc catcgagccc caattcttcg cggagttgct gagcgggctc
      541 ggcctgtcgc ccgacgaggt gcccggccaa ctcgacatcg gttcgtacca acggatgtac
      601 gacgtcttcg ccgaacggtt cgccagccgg acccgtgacg aggggacaca ggttttcgcg
      661 ggcaccgacg catgtgtcac cccggtgttg gcctggcggg aggccggcgc cgatcctcat
      721 ctgcgggcgc gggcgaccgt gatcaccgcc catggcgtcg aacaggccgc gcccgcaccg
      781 cgtttttccc gaaccccgac cggtccggtc gggccgccgc cggcgacaac aacgccgctc
      841 accgaaatcg actggtaatt gtgtgcggcg aacaccatcc gcgcggtggg cgccgttgct
      901 aggttgaact gagtggccgt aaaagcatca cgggagtttg tcgttgacgc gccgccagaa
      961 gtggtcatgg cagcgctgag ggatgtcggc ctgttgtcgt cgtggtcgcc gctacacaaa
     1021 caaatggaag tgatcgatcg ctatccggat ggccggccgc atcatgtcaa ggccacggtc
     1081 aggattctgg ggctcgtcga caaagaggtc ctggaatatc actggggtcc cgactggatg
     1141 atctgggatg ccaaacgaac ctctcagcag cgtggtcagc acgtcgagta caacctcaaa
     1201 cccgagggag tcgacaagac ccgggtgcgt ttcgacatca ccgtcgaacc ggcggggcct
     1261 attcccggtt ttgtcgtcaa gcgggcgact gaaaatgttc tcaatgcggc ggttaaaggg
     1321 ctgagcgatc tcgtcgtggc agacagggat gcgggtaagg cgaagtagtt gccgttttct
     1381 tgcgtccgcg tgcgatcacg attgctaatt tcgtagcagt ggccgtacga gcgtcgtcgg
     1441 aaattgtcat tgatgcgccc ccggaagtga tcatggaggc gctcgccgac atgcctgccg
     1501 tgccgtcgtg gtcctcggtg cacaaacgcg tgatagtcgt cgacgaatat cccgacggac
     1561 gcccgcacca cgtgaaggtc accatcaagg tgaccggcat cggcgacacg gaattgctcg
     1621 aataccactg ggggccggac tggatggtgt gggacgcgaa gaagacggcc cagcagcatg
     1681 gtcaacacgg ggagtacacc ctgatccggg aaggcgacga gaagaccagg gtgcgtttcg
     1741 ccatcaccgt ggagctgtgg gcgccgctgc ccgagttttg ggtcaatcgg gcccgcagga
     1801 agatcctgta cgccgcgctc gagggactgc ggcagcgcgt tttgggtgcc gggcgctcgg
     1861 gtccggctgc ctagcgactc aatgccgcag cgcggccagt cgtctgatcg cttcgtcgag
     1921 ggtgtcgtcg cgtttgcaga aagtgaagcg caccaggtga ttccacacct gggcttgcgc
     1981 cacggcatgg gctgcggccg gatcgcagaa cgccgacatc gggatcgccg ccacgccgac
     2041 cttttccggc agttccgcgc agaacgcggt gctgtcgtcg tacccgagcg ggcgcgggtc
     2101 ggcgcacagg aagtaactgc cgtcgctgtc gtgtaccgca aagccgatct cggtcagccc
     2161 ggcggccagc cgatcgcgtc tggcctgcaa agaacggcga agttcggcca cccaagcgtc
     2221 ctcggtatcc agcgcgaggg ccactgccgg ctggaaaggc gcgcccccga cgtaggtcaa
     2281 aaactgtttg gccgcacgca ctccggcgat gagttgcgcg gggccgcaag cccatccgat
     2341 cttccagccc gtgcagttga acatcttggc cgcgctggac acggtgatcg tgcgctccgc
     2401 cataccgtcg aagccggcca gcggaagatg gcggtggcct tcgggggagg agaacaccag
     2461 gtgttcgtag acctcgtcgg tgatcaccaa aaggtcggcc gccaccgcga tctccgcgat
     2521 ggccgccagc tcgaccgcgc tcagcaccgt accggtcggg ttgtgtgggg agttgacgat
     2581 cagcgcgcgg gtccgcggcg tcaccgcgcg tcgcaaagcg tcggcatcca gagcaaagcc
     2641 gcgcccgtcg gggaccagtg gcacggttac ccggtgtgcg caggccatcg ccaccaccgg
     2701 cgagtacgag tcgtagaacg gctcgatcag gatgacttcc gagcccggct cgaccaggcc
     2761 catcaccgcc gccgcgatgg cctctgtggc cccgaccgtc acgagcacct cggtgtcggg
     2821 gtggtattcg atgccgaaat ggcgccaacg ttgggcggcg atggcgtggc gcagcggggc
     2881 gatgcctatc ccgggcgggt actggttggc accctcggcg atggcgtctt gcgcggcctt
     2941 caacaacgcg gggggcccgt cgtcgtcggg gaagccctga cccaggttca ccgcgccgat
     3001 ccgcgcggcc aaggccgaca tctcggcgaa taccgtcgtc gcgtagggcc gcagtcgcga
     3061 caccgtcatg gcggtccagc atagctttac gcgtcgttgt gaccccagcg ttacgctcgg
     3121 cgccgaacgt gaccgcggcg tcacgttcgg gcgctgacca gcgaaatcct gcccaaccaa
     3181 caggttggcc gggggctttg ttagggtgct gggtagacag acccggaacc caattggcga
     3241 acaggaagtc aacatgtccg aagaagcctt catctacgag gccatccgca ccccgcgcgg
     3301 caagcagaag aacggcgcgt tgcatgaggt caagccattg agcctggtgg tcggcctgat
     3361 cgacgagctg cgcaggcgca atcccgacct cgacgagaac ctgatcagcg acctcatcct
     3421 cggagtggtt tcgccggtcg gcgatcaggg cggcgacatc gcccgggccg cggtgctggc
     3481 ctcgggtatg ccggttacct cgggcggcgt gcagctgaac cggttctgcg catcgggcct
     3541 ggaagcggtg aacaccgccg cccagaaggt ccgctccggc tgggatgacc tggtgctggc
     3601 gggcggcgtg gagtcgatga gccgcgtgcc gatgggctcc gacggtggcg ccatggggct
     3661 ggacccggcg acgaactacg acgtgatgtt cgtcccgcag ggcatcggcg ccgacctgat
     3721 cgccaccatc gagggcttct ctcgtgaaga cgttgacaac tacgcgctgc gcagccagga
     3781 aaagtccgct caagcgtggt cgggcggcta cttcgccaag tccgtggtgc cggtccgcga
     3841 ccagaacggc ctgctgattc tcgaccacga cgagcacatg cggcccgaca ccaagatgga
     3901 gggcctggcc aagctgaagc ccgccttcga gggcctcgcg gcgctgggtg gcttcgacga
     3961 cgtcgcgctg cagaagtacc actgggtgga gaagatcaac cacgtgcaca ccggcggcaa
     4021 cagctccggg atcgtcgatg gcgcggcgct ggtcatgatc ggcagtgaga aggcgggggc
     4081 ggccatcaat tcgacgccgc gggcgcgcat cgtggccacc gccaccagcg gagccgatcc
     4141 ggtgatcatg ctgaccgggc cgacacccgc cacgcgcaag gtgctcgacc gtgccggcct
     4201 gaccgtcgac gacatcgacc tgttcgagct caacgaggcg tttgcctcgg tggtgctgaa
     4261 gttccagaag gacctcaaca tccccgacga gaagctcaac gtcaacggcg gcgccatcgc
     4321 gatgggccac ccgctgggtg ccaccggcgc catgatcctg ggcacgatgg tcgacgagct
     4381 ggagcgccgc aacgcccggc gagcgcttgt cacgctctgc atcggcggcg gcatgggcgt
     4441 tgcgacgatc atcgagaggg tttaagcaca tgtcggacaa cacaatccaa tgggataagg
     4501 atgccgacgg catcgtcacc ctgaccatgg acgacccgtc gggatcggcc aatgtgatga
     4561 acgacgcgta catcgagtcg atgggcaaga ccgtggatcg ccttgtcgcg gaaaaggatt
     4621 cgatcaccgg ggtggtgatc accagtggga agaagacctt cttcgccggc ggcgacctca
     4681 cgtcgatgat caaggccggg ccggaagacg ccggccagtt cttcgacagc gtggaaacgg
     4741 tcaagaagca gctacgcacc ctcgagacgc tgggcaaacc ggtcgtcgcc gcgatcaacg
     4801 gcgccgcact gggtggcggc ctggagatcg cgctggcctg tcatcaccgc atcgcggccg
     4861 acgtcaaggg cagccagctc ggcctgcccg aagtgacgct gggtctgttg cccggtggtg
     4921 gcggggtgac gcgcacggtg cgaatgctgg ggattcagaa cgcttttgtc accgtgcttg
     4981 cccaaggcac ccgattcaag ccggccaagg caaaagagat cgggctggtc gatgaggtgc
     5041 tgccgaccgt cgaggagttg gtgcccgccg ccaaggcttg gataaaggag gagctcaagg
     5101 ccaaccccga cggtgccggt gtgcagccgt gggacaagaa gggctacaag atccccggcg
     5161 gcactccgtc ctcgccgggg ctggcggcca tcttgccgtc gttcccgtca aacctgcgca
     5221 agcagctcaa gggcgcgccg atgcctgccc cgcgggccat cctggccgcc gcggtcgaag
     5281 gctcgcaggt cgacttcgac acagcaactc gcatcgagag ccgttacttc acgcagctgg
     5341 tcaccggcca ggtggccaag aacatgatcc aggcgttctt cttcgacctg cagcacatca
     5401 actccggcgg ctcccggccc gaggggatcg gcaagacccc gatcaacaag atcggtgtgc
     5461 tgggcgcagg catgatgggc gccggcatcg cttacgtctc ggcgaaggcc ggctacgacg
     5521 tcgtgctcaa agacatcagc ctcgaggccg cgcagaaggg caaagggtac tccgagaaac
     5581 tggaagccaa ggcgctcgag cggggccgca ccagccccga gaaatccagg gcactgctgg
     5641 accggatcac cccgacgggt gacccgtccg acctcgccgg cgtcgacttc gtgatcgagg
     5701 ccgtattcga gaatcaggac ctcaagcaca aggtgttcca ggagatcgaa gacgtcgtcg
     5761 agcccaacgc ggtgctcggg tccaacacct cgactctgcc gatcaccggt ctggcgaccg
     5821 gcgtcaaacg ccaagaggac ttcatcggga tccacttctt ctcgccggtc gacaagatgc
     5881 cgctggtgga aatcatcaag ggcgagaaga catctgacga gacgctggcc cgcgtgttcg
     5941 actacaccct ggccatcggc aagacgccga tcgtcgtcaa cgacagccgc gggttcttca
     6001 cctcccgggt catcggcacc ttcgtcaacg acgcgctggc catgctcggg gagggcgtcg
     6061 aacctgccag catcgagcag gcgggttccc aggctggtta cccggcgccg ccgctgcagc
     6121 tgtccgacga gctcaacctg gagctgatgc acaagatcgc ggtcgccggc cgcaaggctg
     6181 tcgaggacgc cgggggcacc taccagccgc acccggcgga agccgtcgtg gagaagatga
     6241 tcgagctcgg acggtccggc cggctcaagg gcgcgggttt ctacgagtac gtcgacggca
     6301 agcgaacccg cctctggccg ggtctgcgtg aggtgttcaa atccgggagt tcgcagccgc
     6361 cgctgcagga catgattgac cggatgctgt tcgccgaggc cctcgaaaca cagaagtgcc
     6421 ttgacgagga tgtgctgacg tcgacggtcg acgccaacat cgggtcgatc atgggcatcg
     6481 gcttcccgcc gtggaccggt ggcagtgcgc agtacctcgt cggctactcg gggccggccg
     6541 gtaccggcaa ggaggccttc gtggcccggg cgcgtgagct ggcggacaag tacggcgagc
     6601 gctttctacc gccggactcg ctggtcaact gaaaccgcgg gctcagccgg tgcgttgcag
     6661 cacatagcag tgcaccacga atcggttgcg aacccacgcg accggtgtaa atcgcgttgc
     6721 gggtccttcg ccaaacggca gtcgttcatc ggtcgccgac agcagcttcc atgccggcgt
     6781 gaaccggtgc tcgacctccg cctcgtcgag tcccagaaat ccgatcgggc caccgggttt
     6841 ggcggcgacg atcaacagcc gtgcgccagg ggcggcggcc gctgtgatct cccggacata
     6901 gaggtcacgg tcgtcgtcac tcataccgtg aaggcagccg ttgtcgacga tcaactgaaa
     6961 cggtgtgtcg agtccggctt ggcttagatg tgtgacgtcg gcgtggatga agtcgaccgg
     7021 cacgccggca gtacgtgcct tggcgcgggc cttatcgagc gccttcggga cgaagtcgac
     7081 ccccgtgacg tgccatccat gccgggcgag gtataccgac gagtcgccgg tgccgcatcc
     7141 gacgtccagc gccgcttctg gcggcaaggc gggtatatca gcggttccct cgatcagctc
     7201 ccgcagccgc tccgaaagcg catgaccgtc ccacggggtg aacccgagcc cgtaaaagat
     7261 ctggaacaag ctgtgtcgag aagccatgac tcacggtatt ccgcgtagcc gctagccggc
     7321 taccggcgag ctgtactcct tgaactgcat cgagtcgtag agtcgcaccc ctttggtgtt
     7381 cagttttgcg actgctttgg tgagcccggc gggcagtaac gagatcactt gcgcgccacg
     7441 ggtcagcgcc cagactccgg ctcttgagtt tggaacgagg ctcttggcga ttccgcgcgc
     7501 catagcgcgg ctgcgacgca ccggatctgc catctcccgc tcgtatgccg cgaacgcctg
     7561 cgcgtagtct ccgcgcgctt gcgccaattc acccgccagc acataggcac ccagcacggc
     7621 gatgctggtg ctgccgccta ccgcgggccc cgggcagtat ccggcatcgc cgaccagcgt
     7681 gacccgtccg cgcgaccagg tgtccaacag caactgcgtg atcgagtcga agtagaacgt
     7741 cggtgtgcgg tcgagctcgt caagccaccc gtccaccgtc tcgtgcatcc ccgcgaacgc
     7801 ggtgcggagt aattcctgct gccgttgggc gtctcggtag tgatattcga gttcttgatt
     7861 gctgcggaac atgaacaatg cccgtgcgtc atccagcggc tgcgcggtgt agattcccgc
     7921 gagccggccc accccgacgt ggacgaccat ctccccctcg cgcgcaaggg ttttgggtac
     7981 cgacagaacc gccaggtaac cgccgaggaa ctgggtgcgg ccggcgtcct ggccaaagac
     8041 caggcgccga acgttcgaat gcagcccgtc ggcgccgatc accacgtcga accagcgtgg
     8101 cgccgcgtgt tcgaaggtca cttcaccgtc gggggagatg gccgtgatcg aatcgccgaa
     8161 cagatactcg acatggtcgc gtccagcgtc gtagtaggcc tcgctgaggt cgtctcgcat
     8221 gatctctacg tgccggtcgg atgtggcgcc gacgactttg gtgagatcga tgcgggccgc
     8281 ccgccgcgcg ccctcgcgat acatcgtcaa cacgtcggtg ccggtggcca gcgcctcgat
     8341 cttgggcaac acacccatct tctccgagat ctccatcgcc ggccggaaca ggtcaacggc
     8401 atgaccaccc gtcttccgta gtgccggcgc gcgttcgacg acagtcactt cgaacccgta
     8461 ccgcgtaagc cagtacgcga gcaccggccc ggagatgctg gcgccggaga tcagaatccg
     8521 catggcaacc tccgcactta cttaacgttc ggtaagcgta ccatgcatgc ttacctatcg
     8581 gtaagtcagt taagctcgaa gagtgagtcc gccacggtca gacaccaggc agcgaatcca
     8641 agacgtggcg cgcgacctgt ttctgcaaca gggcgtgcag cgcaccagcc tgcaggacat
     8701 cgcggacaag ctgggaatca ccaaacccgc cttgtattac cacttctcgt cgcgagaaga
     8761 cttggtgcgc agcatcctgg tgcccctaat cgatgaaggc gagcaattcg tcgatgagca
     8821 ggaggctcgc ggccatacgg atgcccgcga gttgttgggg ggctatttcg atttccacta
     8881 ccggcaccgc gaggacctgg tgctggtgct ggccgaattg acagcgctgg ccgaccttgg
     8941 cctcatcgac aaggtactgg cttggcgtga ccgcctcggc aagctggtgt tcggcccgcg
     9001 gccgacgctg gcgcagtcca cccgcgcggt ggtggcgttc ggcggtttgc aggactgctg
     9061 cctgcagttt cccgacaccc cgtaccagga gctacgtgcg aactcggtgg aagccgcact
     9121 tgccgcactg ggcaagtgag gccaggcctg ggcgatctgc aacgtgacga tgccgaaata
     9181 gttcctgaca cagaaactct cgtgcgcgct ggcgttatgc ggtgctctgg tcctgggcgt
     9241 tttgggtgaa gatgtcgccg gagcttgggt tggtgtcgtc gtcgacgttg atcgcgctcg
     9301 ccgcaccaga tgtgttctgc gcgagctgca taccgatggg agccgtgagg ggtgccggcc
     9361 cagacgcggt attggcgtgt gtggcactcg gccccgcggc gctgaataat gcgagcgtcg
     9421 cgacggcggc tgcgccggcg aacagcaact gcatcacccg gatttgccga cgccgcgcgt
     9481 cggccgaatt gctttcgttg gcaggtgggg tggatcgctc atttgtcatg catcacatgt
     9541 acagcctgac tgccaggagc gcgctaagcc cttgttattc gttcgctgtg aggttggggc
     9601 ggcacggcgc cagcatcgtc gacagctaga gtcactaacc atgcgcttcg gactgttcat
     9661 tccgcagggt tggcgaatgg atctggtcgg catcgacccc gcaagccagt gggcggtgat
     9721 gcgcgacctg gccacttacg cggacggcag cgcgtgggac tcgctgtggg tctacgacca
     9781 cttccatacc gtcccgctgc ccgcgggcga ggccacgcac gaggcgtggt cgctgatggc
     9841 ggcgtacgcg gcgacgactt cgcggatcaa gctcggtcag atgtgcacgg cgatgagcta
     9901 ccgcaatccg gtctacctgg ccaaggtggc ggccaccgcc gacatcattt ccggcggccg
     9961 aatccaaatg ggtatcggtg gcggctggta cgaacatgag tggcgcgctt acggttacgg
    10021 ctttccttcg gcgggcgtgc ggctgggccg gctggatgag ggtgtgcaga tcatgcgcga
    10081 cgcctggcgg gacggcaaag taagtttcaa cggtaagcac tatcaggtcg atggcgcgat
    10141 tgtcgcaccg aagccgttgc aggacaatgg cattccgttg tggatcgccg gcggtggcga
    10201 gaaggtgacc ctgcgcatcg ccgcgaagta tgcgcagtac accaatttca ccccggcgct
    10261 cccggagttt gcgcacaagt ccgaggtgtt ggcagaacac tgccgcgagg tgggcaccga
    10321 cttcgatgcg atcgtgcgct cggccaactt cagcgtgatc gtcggcacct ccgacgccga
    10381 aatcaacgac cggcagcagc gcatccgcgg tcggctttcc ggctatgtgc ccgacgccgt
    10441 gctggattcg atgatcgccg ccagcagtgg cccggattcg atcacgggca caccggaaca
    10501 ggtggtcgag cggatcggca aagtccgcga gctcgggtgc gactacgcga tctgctactt
    10561 cccggaagcc gcctatgacc gctccggcat cgagttgttc gaacgcgaag tgattcccgc
    10621 cctgagctga gctagacgtc ggcggtgacg tacctcgtct tcaccggagg cgcggccagc
    10681 agcggaggaa gttcggtcac tttgccttcg ccggcacgga atttgcggta ggcctcgtcg
    10741 tgctcgacgg tgtcccagag ctcgtagata agccagtgcg cttcgtcgtc ctgatcgacg
    10801 agcacgtcgg tgccgctgtt cccgtcgaac gctcgggtaa cttgcagcgt ccggcgcatc
    10861 aggtcgcgcc ccgcagacac cgcgtcgggc ttgaatctga gttcgagtat caccgtgacc
    10921 ggcatagttg cctcctcgca ccggagtcgg gttccccgtt tggggctccg acacgataac
    10981 tccccgtgat atgccgacgg ccggcgggct cggtcggcgc cagtcacatt tgccacacac
    11041 gtctctcggg tggggatgtc cagtgtgccc gcctccgcgc gacaatggcg gtcgttggcc
    11101 cagaggcgac cgggttgtcg ctcagaggtg ggcaaattgt cggccgtctg cgccacaaac
    11161 ccgtggggcc agtgaggcct ggccgtccgc cgagccatcg cgagaacgtg gtttccattt
    11221 ttctcgaatg ctgttgtacc ttttaggaag cgcatatgtg ttgcggaaga cggaggactc
    11281 ctcgatgcag aaggcactcg cccccgaaat ctccacctgg cccgacgaga acccgcagct
    11341 gatcggaagt cgatgcggca actgtcgtgc caccacgttc ccggtgcagc agtggtgtcc
    11401 gcgctgcagt ggcggtgaga tgaccgcgtt gttgttgccg cgccgcggaa ccctggtggc
    11461 gtggaccacc cagggtttcc cgcccggggc tccttacgcc ggtcccaccg gcaaggattt
    11521 cgtgccgttc ggcgtcggcc tggttcagct tggcctggga gaagacgctg tcatccgcgt
    11581 cgagggccgg ctgaccgaga acgacccggc caagctgcgg ttcggccagg aggtcgaact
    11641 gaccatggtg ccgttgacca ccgacgaaga gggcgccgaa gtcatgacct tcgcgttccg
    11701 gccggtctag tggcgatcgc gagcgcggca gtggcgatcg cgagcgcggc cgtggcgatc
    11761 gcgagcgcgg cgaagccggg cgaagtgggt cgccaccata aggaggagcc gggtgaagcg
    11821 ggtcgccacc atctatccag aaggaggccg cgatgaccaa cgacgtagcc atcatcggcg
    11881 tgggcctgca tcccttcggc aggttcgaca agaccgcgat gcagatgggg gccgaggcca
    11941 tccaatccgc gctgaccgac gccggtgtgg actggaagga catccagttc ggcttcggcg
    12001 gcagctacga agtgtccaat ccggacgcgg tgacccgctt ggtcgggctg accggcatca
    12061 cgttcaccaa cgtcttcaac gcctgtgcca ccgcggccag tgccattgcg cagaccgccg
    12121 acaccctccg gttgggcagg tacgacatcg gcatcgccat cggcttggac aaacatccgc
    12181 gcggcgcgtt caccgatgac ccggccaagc tggcgctgcc gcagtggtac gcgcagaacg
    12241 ggcagttcgt caccaccaag ttcttcggga tgaaggcgaa caagtacctg cacgaccaca
    12301 acatctccca gtccacgctg gccaaggtgg ccgccaagaa cttccgcaat ggcgctttga
    12361 acccaaatgc gtttcgccgc aagccgattc ccgaaaacga gatcctcaac tcggcggtac
    12421 tcaactatcc gctgactcag tacatgttct gcgcgcccga cgagggcgcc gccgctgtgg
    12481 tgatgtgtcg cggcgacatc gcgcacaagt tcacggacaa gccagtgtat gtgccagcct
    12541 gcgagatccg tacccggcgt tacggcgcct acgaggtgca cgccacgtcg gcgccgctgg
    12601 acgaggacgc ctcgccgacc gtgtacgcgg ccaaggccgc ctacgaggcg gcgggcatcg
    12661 ggcccgagga tgtcgacatc gcgcagctgc aggacaccga cgccggcgcc gaggtgatcc
    12721 acatggccga gacgggtctg tgcgcagacg gcgagcaaga gaaactggtg gccgacggcg
    12781 ccaccgagat tcacggctcg atccccgtca acaccgatgg cgggctgatc gccaacggcg
    12841 agccgatcgg cgcgtcagga ttgcggcagg tgcacgagct ggttcgccag ttgcgcggcg
    12901 aagcgggcga gcgtcaggtg cccggcaacc cgcgcgtcgg ccttgctcag gtgtacgggg
    12961 cacccgggac cgcgtcggcg accatcctgt cgctgtagat tgctcacggg gcagccctaa
    13021 attgccgggc ccagcaggtc gtccgcatct cgaatgatgt agccgtagcc ctgctctgcc
    13081 aagaagcgct ggcggtgcgc ggcatactcg gcgtcaaggc tgtcgcgggc caccacggag
    13141 tagaagatgg cacctccgcc gtcagacttg ggccggagca gccggcccag ccgctgagcc
    13201 tcctcttgcc gtgagccaaa ggtccccgaa acctgcaccg ccacagacgc ttccggcaag
    13261 tcgatggaga agttggcgac cttggacacc accagtgtcg acacctcgcc gcggcggaac
    13321 gcgtcgaaca gcgcttcgcg atccgtggtt cgggtggacc cctggatcac cggagcgttc
    13381 agctcggcgc ccagctcgtc gagctggtcg aggtaagcgc cgatcaccag ggtctgctcg
    13441 ccgggatgtt tctgcagaat cgatttgacc acagcaattt tggtgtgcac cgtcgagcac
    13501 agccggtacc gttcttcggg ttcagcggtg gcgtacgtca ttcgctcgct gtcggtcatg
    13561 gtgacccgga cttcgacaca ctccgccggc gcgatccagc cctgagcctc gatgtctttc
    13621 cacggcgcgt catagcgctt cgggccgatc agggaaaaca cgtcgccctc gcggccgtct
    13681 tcgcgaatca acgtggcggt caggcccagt cgtcgcttgg actgcaggtc ggcagtcatc
    13741 cggaacaccg gcgcaggcaa cagatgcacc tcgtcgtaga tgatcagccc ccaatcgcgg
    13801 ctttcgaaaa gttccaggtg ccggtactca cccttggtgc ggcgggtgat catctggtag
    13861 gtcgagatgg tgacgggccg gatttccttg cgttctccgg agtattcgcc gatctcgtct
    13921 tcggtcagcg aggtgcgcgc aatcagctcg cgtttccatt gccgggcggc gacgatattg
    13981 gtgaccagga tcagcgtcgt cgcaccggct ttcgccattg cggccgcgcc gaccagcgtc
    14041 ttgccggcac cacacggaag taccaccacc ccggaaccgc ccgcccagaa cgagtcggtg
    14101 gccagctgct ggtaatcgcg caggtgccag ccgtcctcgt gcaagctgat cgggtgtgcc
    14161 tcaccattga cgtagccggc gagatcatct gcgggccaac cgattttgag cagcatctgc
    14221 ttgactcgac cgcgctcgct ggggtggatg acgacggtgt cggcgtcgat gcgggcaccg
    14281 agcatcggtg cgatcttctt gttgcgcagc acttcctcga gcaccgcgcg atcaaagctc
    14341 accagcgtga ggccgtgcgc cgggtgtctg accagttgca gccggccgta gcgggccatg
    14401 gtgtcgacga tgtcgaccag cagcggctgg ggcaccgcgt agcgcgagaa ggagaccagc
    14461 gcgtcgacga cctgttcggc gtcatggccg gcggctcggg cgttccacag cgccagcggg
    14521 gtgatgcggt aggtgtgcac gtgttcgggt gcgcgctcca gctcggcgaa cggtgcgatg
    14581 gcggcgcgcg ctgcgccggc cagctcgtgg tccacttcca gcagcaccgt cttgtcggac
    14641 tgcacgatca acggcccgtc agtcaatggc gccgctcctt ttcatcactg cgttctgcgt
    14701 cgtcgccggc tccggccatg tgcgccgctc ctcctcatca ctgcgttctg cgtcgtcgcc
    14761 ggcgcgaatc atctgcccca ttattcagtc gtccgccgac accaccgagg tgatgcggtg
    14821 gatggcgaag tctcgcagcc gcccggacgc ggaatcgaag gccaccagct gcgcgccgcg
    14881 cacggtgatc ggagacacca cccgctgggt ggccacgccg gcggcgtcca ggtaaccgat
    14941 catcaacgtg tcccggtctt tggccgcgcg ctgcaacagc gacatggtga ccgccgggtc
    15001 gatgcggata ttgccgaagg gtgcggcggt caccttacgc agcaccgaga cgacggcgtt
    15061 gagtgtttcg ctgctggggc gcggcagggg gcggtacggg cggcgctgct ggggcgcggg
    15121 cacccgggct ccgcgaggcc ggacgtcgac gatggagccg gtggaatctt cagccgccgg
    15181 ggcgaaaccc gcggcccgca acgcgaccag aacgtcggcg atcggcgcgg gcgacactgc
    15241 caccgtgggc gccagggcgc gcagctgcag ctctccagcg gccgccaccg cctgcgccaa
    15301 cagcgcgggg tcctcgcacc gtacgaagga agcggccatg ccgatgcgga gttgaccatg
    15361 ccgacgtgcg acgtcgtcga tcaggtaggt aagcccttgc ggcaccggag ttttggaatg
    15421 cctgcggaaa agttcgtgca tccagtcacg agtcttgccg atgtccagag cgtgccggat
    15481 cgactgctcg ctgatgcggt acaccatcgc cgtgccggcc gattcgacgg tggcgaccga
    15541 ggcaaggtct tcggcgaggt cgcgttggag tggcccgggt acgacgacgg tcaggtcggc
    15601 ctgcaccaga aagtggtcga ccggtgcggg caacgcccgc gccatcgcct cgaccgcgac
    15661 ctcgggcgcg acggcttcgt ccagcagcgc gcgcccaggc gtgctgatcg caccgcggcc
    15721 tgccagaccc atcgcgtggc tctcggccag cagatcggcg acggggtgcg gctgcagccg
    15781 cctggtccag cgtggacgtc gccagatcag cgccgccgag gcggctgacg catcgacgcc
    15841 ggcaccgggg ggcagctcgg agagcatgcc caacagcagc cggcgatcca gcggagcggc
    15901 cgtcgagtag agcgaatcag taagagcgcc atatggtttc gcatccggac cgcgaccgcc
    15961 gatcaacgct ggccgggccg gcaactcgag ccaggtgctg gccaacaggt gccagcgttc
    16021 ggcgggggac atcgccgcaa accgttcggt ggcgaccgtt ggcgcccaat aaggagcatc
    16081 gccatcaagc ggctgcggat cgggcatgcc gctggcgatc aatccggccg ccgccgccac
    16141 ctcgaggatc agccccagcc ggggttcccc aatgccggta gctttggcta gccgcttgac
    16201 gtcacgaatt cccagtccgc cgctgcgtaa ttcggaaacc ggtgcagcgc caagggtttg
    16261 aagtaggacg tcgatctctc gcagcaggtc gatgacggct ccggcggccg ccgcatcgac
    16321 atcgtcgggt gtggttttcg atgccaccgg atcgggcgcc gtcagctgcg tcggtccggg
    16381 attttcgccg cgcagcacct gcccgacgtg gcggggcagg atcaccgtct ctgcatcgat
    16441 gcgtcgcagc aggcccatcg caagcaactg tggcaccggc cgatccgcag gagcaccggg
    16501 cgcggcgtca cgggtgcggc ccagcggaga accttcgagc agtttttcca gcacttcgag
    16561 ctgggcctgg tcgaggccgt cgatgagacc agcgatctgt tcgccggtgt gtgaagtgtc
    16621 ctcgagcatg acctgccctg gatgccacgg caatgcggag gcggcgtcgg cggccacccg
    16681 gagcgaggcc tcgccccaaa cgagggcacg cgacctgagg tcatcgagcg cgcccaccac
    16741 gtcggcttcg ggcacccggt caccgatgag cgcgagcagc tttgcggtcg ataccggcgc
    16801 cgaatcggcc tgcagcacca gcagcgcgtc gagcaccgcg agccgcaaga agtcgagatc
    16861 gtcggtggca gccttcaccg actgacgagc ctgggcgcgc gcggcgagtg cggcgatgct
    16921 gccgggcggg ggctgggcca ggtccgggcg caattccagc agtcggatca accgatcgtc
    16981 gggcaagtcg gccagccagg accccagcgg gatatccggg gtgttgtcgg tcattgctga
    17041 ccagcgtaaa gcagtcagcc gacgcgaagg ttgcgatcga gacgtggtgg ttgacgtgca
    17101 atggtttcgc cggctcaggg gacgacgggc aacgtcgagt aacgccttgc gcgacacgcg
    17161 ggggtgcgac ctgtcagaat gatccccgtg gcggacattg ctgaggacaa ggcgagcaaa
    17221 acaaggtacg tcgacaatgg ctggccgacg acggaccccg acgaccacgc ggtgagcgaa
    17281 ctggtgagcg atcgaacggg tgcgtcatca ccgttcggcg aactggtgtt tccgctgccc
    17341 gcgaccgacc tgccctacgt tcacccggtc accgtcgtca acagataggt cgcccggatg
    17401 gctagggcct ctgaggccgc taagccctcg gcggcctggg cgtcggggga atcacgcccg
    17461 ggcacgctgt cgcacctgga tgagcggggg gcggcgcaca tggtcgacgt aacgggaaag
    17521 ggagccacca agcgcaccgc agttgccgcg ggcgccctgc gtaccacgcc gcaggtggtg
    17581 gcgctgatct cgaccggcgg cctgcccaag ggtgatgcgt tggccaccgc tcgggtggcg
    17641 gccattctgg cagcgaagcg caccagcgac ctgatcccgc tgtgccacca gctcgcgctc
    17701 accggggtgg acgtcgattt cgtcatcggc gaatcggaca tcgagatcac cgcgacggtg
    17761 cgcagcaccg accgtacggg cgtcgaaatg gaggcgctga ccgccgtcag tgtggccggg
    17821 ctcacgctgt acgacatgat caaagcggtg gaccccgccg cccgtatcga tgacgttcgg
    17881 gtgcttcgca agcagggcgg caagagtgga agttggacac ggtgacgagc cgccgatccg
    17941 cacgcgtaat catcgcctcg acccgcgcgg cggccggcgt ctacgacgac caatgtgggc
    18001 cgatcatcgc ggaatggctt gagcagcggg ggttttcggc ggtgcagccc gaggtggtcg
    18061 ccgacgggca gcttattacc aaagccctgc tcagcggggt cgacgcggga gtcgacgtga
    18121 tcatcacctc ggggggtacc ggcatctccc caaccgacag cacccccgac cagaccgtgg
    18181 cggtgctgga ctacatgatt cccgggctgg cggacgccat ccggcggtcc ggtctgccga
    18241 aggtgccaac gtcggtgctt tcgcgtggcg tgtgcggagt ggccggcggg acactgattg
    18301 tcaatctgcc gggttcaccg ggtggggtgc gagacggcct cggggtgctc gccgatgtgc
    18361 tcgaccacgc gctcgatcaa attgcgggcg gggatcatct gcgatgacac gcgtcctgcg
    18421 tgccgcaatt accgaagagc cgatctttct cgcagaacac gaagaattgg tcggccatca
    18481 gtcggccgga gcgatagtcg gcttcgttgg catgatccgc gatcatgacg gtggccgcag
    18541 ggtagtgcgg ctggagtatt cggcgcaccc gtcggctgcg caggtcatcg cccaactcgt
    18601 ggccgaggtc gctgaggagg ccagcggggt gcgcgccgtc gcggccagcc atcgcatcgg
    18661 tgcgctgcac atcggagagg cggcgctggt ggccgcagtc gcggccgacc atcgacaggc
    18721 ggcgtttgag acctgcgcac gactggtcga caccatcaag gcgcggttgc cggtgtggaa
    18781 acaccagttc ttcgacgacg gaaccgaaga atgggtgggc tcggcctaga gcgtggttga
    18841 cgtccccgga caatcgggga tgccggcgct ctcccgcagg aggaggtttg cggcagagcg
    18901 ccgggcgatc cggctgggga gagacgtcag ttgatgccgg acggctgcgc aacagcgtcc
    18961 agcgcatcgt tcccgtggat gtcggcgtcc cggatggcgt cccacagctg cctcgtgtag
    19021 ccgaccttcg acgcgtgctg tggttcagcc ggcgcggcgt cgacgtcacc cgggggcggc
    19081 ggcacgtcgg ccggtgcggg agccgggaag gcagcgtctg ggggtggcgc gtccgcaggg
    19141 ggcgccggcg gtgccaggtc cgccggaggc gcaggcggca cgtccgcggg gggcgccgct
    19201 tggtcgaagc cggccagctg cacgggtgca ggcgggtcag ccggcggtgg ggggggcggc
    19261 ggaggaggag cgtccggcgg gggagcatcc gggggcgggg gtgccagcgg tgccggcaag
    19321 ccgttgagcc acggcaggtc cagtggcgcg tccatccccg tcggtgcggg gacctcacgc
    19381 ggggtcggtc ccgacagcgg accgccacaa acgggccagg cgccacgacc ctggtcagcc
    19441 agcacccgct cagcgaccgc gatctgctgg tccttggtgg ccagctgggc cgacggagcg
    19501 aactcgccgc cgccgtgcga ggaccaggtg cttgcggtga actggacacc gccgaaatac
    19561 ccgttgccgg tgttgatgcc ccagttgccg cccgactcgc agcgggctac ctgatcccac
    19621 tcgccgtcgg tggcagcggc ggcctggccg gccatggcga tgctgccgcc gccaagtact
    19681 gcccctgtga aggcaatctt ggcgacgctg atgctcgaag ttgtgggctt acggtgacgt
    19741 ccactcatat gcgccgcgat attcctctcg tgacgcgcct gcgaggtcag ctgtcgggtt
    19801 cgggttagag aggccacccg gccgatgtcg tgctccgcat ggaagcgaca ccgacttcac
    19861 cccaaggaac cgctggcggc tccagtccga tctcggtgga ccggtgggtc ccccgcctcc
    19921 atccaattgc cgtgcccccc gcctattgga tggagctcgg cgcgataggg agggggagga
    19981 acgcactctg ggttggcgag ccttcggaga aggtagcggt ttccgttggg ctcgtcacct
    20041 tgaggcgaac caggtgtttc ccccacggtc acctgcggaa acccgtgaaa caccggcagt
    20101 ttgcgcaggt catagcatgc gagaccgaag cgtgtccggg tcgttatcgt tccgttacgt
    20161 gagatatgtc acatttttag ccgccggcga agggcggtag cacatcgatc gtgtcaccgg
    20221 agcgcaacat tgtggtctcg tcgcggacgg cgatcccgtc acggaggtat gagcaccggc
    20281 tcaacaccgt ggcgagacgg cttccccgga tcgcgagcct gtcgaccagc tcggccaccg
    20341 tggtgtccgg ccgcaccacc agcgtttccc attccgtgcc cgcggcctcg cacgcggcgg
    20401 cgaagtagcg gaccgtcacc tggatcccct ctgcctcggc ggcggtttga cccgtggggc
    20461 tagccaccga tggcgctcat cgggcggtcg ggctggatga agttggggtc gttgatgccg
    20521 tggcccgcgg gcttggccca catcgcggcg cgccaggccg cctcgatcgt gtcgtcctgc
    20581 gcaccgcccc gcagcagtcc gcgaaggtct gtctcctcgg ccgaaaagag gcagctgcgg
    20641 atttggccgt cggccgtgag ccgggtgcga tcgcacgtcg agcagaaggc gtgcgacacc
    20701 gacgcgatga ggccgaactt tccgctgggc gtgttcgggc ccgtgtcgac cacccagagc
    20761 tcggccggcg ccgaaccgcg cggcgccggg tccgggcgca gccggaagtg cggccgcagc
    20821 gccgccagca cgtcgtcggc ggtcagggcg gcctcccggc gccattgatg gccggcgtcc
    20881 agtggcatct gctcgatgac ccgcaactgg tagccgtgct cgaggcagaa cctgagcagc
    20941 tcgacgacgt cctcgcggcc ggttaccgca tcgagcacgg cattgacctt gaccggtgcc
    21001 aggcccgcct cgctcgcggc ggccaagccg tgcagcacct cggtgagccg atcccggcgg
    21061 gtgatggccg cgaagtggtc ccggtccacg ctgtccagtg acacgtttac ccggtccagg
    21121 cccgcctttg cgagcgcggt tgcccgtcgc gcaagtccca cgccgttggt ggtcagcgag
    21181 atctcggggc ggggccgcag gccggccgcc gcggcgacca catcttcgag gtggcgtgac
    21241 aacaaaggct caccaccggt gaaccgcacg caggtgatcc ccagtcgggt gacggcgata
    21301 tgcatgagcc tggccagctc ctcggggcgc agcagctgct ctccgggcag ccagtccagg
    21361 ccgtcggcgg gcatgcagta agtgcaccgc aggttgcagc gatcggtcag cgacacccgc
    21421 aggtccgtgg cggcccgccc gtacgtatcc agcagcggtc ccgcggtggg tgcgtgccgt
    21481 gcccggtcag cgacgatgcg cccatccgcc ggggccgcca tccggctggg caccgtcggt
    21541 aggcccaatg cggtcagcgt catgcgggcg cctgggcgaa tcgatgcgac gaatcgacgg
    21601 gaacgatctg cttgcccagc ggcatcagcg acaccgggat caatttgagg ttggccagcg
    21661 ccaacggaat gccgatgatc gtgagcgcca tcgcgaccgc gctcaccaga tgaccgattg
    21721 ccagccatat gccgaacagc agcacccaga tgacattgcc tagcagggcg ccggtcccgg
    21781 ctgtcggttt gtcgacgatc gtgcggccga aaggccacag cgcgtacgag gcgatccgca
    21841 gcgacgcgac gccgaagggg atggttatga cgagcaggaa gctgatgagc gctgcgagca
    21901 cgtatcccgc ggccatccag aggccgccga acagcagcca gatgacgttc aggaccaggc
    21961 gcataactcc tccagcggta gtgccagcct accgagtaac gagtaacggg ggtgctcgtc
    22021 aggcaagcat ccgagtagga ttacaactca acaatgcgtc ctgcgcaggc ggggcgcttc
    22081 ttgcgtcgcc catcctagtg atccgtcaga caagcaggtg agacagtgcc gagcggcaag
    22141 gttaagtggt acgacgccga gaaggggttt ggcttcctgt cacaggagga cggcgaggac
    22201 gtctacgtcc gctcgtcggc tttgcccgct ggggtcgagg ggctcaaggc ggggcagcgg
    22261 gtggaattcg gcgtggccgc cggccgccgt ggaccgcagg cgttgagtct gaaactgatc
    22321 gatccgccgc ccacccttgc caagacgcgc cgcgaggcgc ccgtcgagca caagcacagt
    22381 cctgatgagt tgcacggcat ggtcgaggac atgatcacgc tgctggagag caccgtgcag
    22441 cccgagttgc gcaagggtcg ctaccccgac cgcaaggccg ctcgccgggt ctccgaggtg
    22501 gtcaaggcgg tggctcggga gcttgactcg taggctgggc tgtggtcggg ttcggagcgg
    22561 tgcaaagatg ctccgggtag tccgctctaa aaccgttcgt ttctgtgctg tggtatcacg
    22621 gccaatagac tgagagtcag gaggcgtcga tggcgcagca aacacaagtc accgcggagc
    22681 aagcacgagc gcttgccgag gagtctcgcg aaagtggttg ggataagccg tctttcgcta
    22741 aggagctgtt cctcggtcgt tttccgttgg agctcattca tccttttccg aggccgtccg
    22801 acgctgaaca ggcgcggacc agcgcgtttc tgggcaaact tcgggagttt ctcgacacca
    22861 tcgacggctc ggtcatcgag cgcgatgccc ggatccccga tgagtatgtg aagggcctgg
    22921 ccgagctggg ctgcttcggc atgaagattc ccgccgaata cggcggcttg cacatgtcgc
    22981 aggttgccta caaccgcgcg ctgatgatgg cgtcgtccgt tcatcccagc ctcggcgcgc
    23041 tgctgtcggc ccatcagtcg atcggggtgc ccgaaccgct caagctggcc ggtacacccg
    23101 agcagaagca aaggttcctg ccgcgctgtg cggcaggtgc catcacggcg ttcctgctca
    23161 ccgagcccga cgtgggttcc gacccggccc gcctggcctc cacggccacg gtgatcgagg
    23221 acggcgcggc ctacgagcta gagggtgtga agttgtggac caccaacggc gtggttgccg
    23281 aactcttggt ggtcatggct cgggtgcctc gcagtgaggg gcaccggggc ggtatcagcg
    23341 ccttcgtcgt cgaggccgat tcgccgggaa tcaccgtgga acggcgcaac aagttcatgg
    23401 gattgcgcgg catcgaaaac ggcgtcacca ggctgcaccg ggttcgggtg ccccgcgaga
    23461 acctgatcgg tcgcgagggt gatggtctga agatcgcgtt gaccacgctc aacgccgggc
    23521 gcctgtcgat acccgccatc gcgacggggt cggcgaagtg gtcgctgaag atcgcgcgcg
    23581 aatggtcggg cgagcgggtg cagtggggca aaccgctggg caagcacgaa gcggttgcca
    23641 gcaagatttc gtttatggcc gccactaact acgcgctcga tgcggtgctc gaactgtccg
    23701 cgcagatggc cgacgaagga cgcaacgaca tccggatcga ggccgcgctg gcgaaactgt
    23761 ggtccagcga gatggcctgc ttgatcgccg acgacctgat acagatccgc ggtggccgtg
    23821 gctacgagac cgccgaatcg ctcgcggcgc gcggggagcg cgcggtaccg gcggagcagt
    23881 cgtggcggga tctgcggatc aaccgcatct tcgagggctc cagcgagatc atgaggctgc
    23941 tgatcgcccg ggaggcggtc gacgcccacc tgaccgccgc cggtgacctc gccaagcccg
    24001 actccgattt gcggcagaag gccgcggcgg cggtgggcgc cagcgggttc tacgcaaaat
    24061 ggctgccaca gctcgttttc ggcgaaggac agcggccctt ggcctaccac gagttcggtc
    24121 ggctggcatc ccacctgaga ttcgtcgagc gctcctcgcg caagctggct cgcaacacct
    24181 tctatgggat ggcgcgctgg caggccgatc tggaattgaa gcaaggattc ctgggacgcg
    24241 tcgtcgatat cggtgcggag ctgttcgcca tgtccgccgc gtgcgtccgg gccgaggctc
    24301 agcgggcggc tgatccggtt gtcggccggc aggcctacga gctggccgag acgttctgcg
    24361 agcaagcgac cctgcgggtc gaggcgttgt tccacgggtt gtggtccaac accgacagca
    24421 gcgacgttcg gctgaccgat gcggtgctgg aaggccgcta cacctggctg gaggacggga
    24481 tcgtcgacca gtccgagggc accggtccgt ggatcacgca ctgggagccg gaggcttcgg
    24541 ccgagaccaa tttggcccga cgatttctga cggtccacgt cccaagctag gcgcacccgc
    24601 aaggcaacgt cgatccgcgg aagtggttgc gcggcaagcg gttcgacgtc ggtgcgggtt
    24661 ggtctctcca gctagcgctg gcagtgagga gaatcgtcgt catgacttcc tacgttgccc
    24721 cggcgggcga attcacgcgc gataccaact acatctccac ccggatcacc gccgacgggc
    24781 gtgacggtta tccggtggag ccggggcggt atcggctggt cgtggcccgg gcatgcccgt
    24841 gggccaaccg cgcgatcatc gtccggcgct tgctggggct ggaagatgtc ctctccattg
    24901 ggttttgcgg ccccacccac gacgagcgga gttggacgtt cgacctggac ccgggcggcg
    24961 tcgacccagt gctgaaaatc ccacggttgc aggacgcgta tttcaagcgc atccccgact
    25021 accccaaggg catcacggtc ccggcgatcg tcgacgtgct caccggcgcg gtggtcacca
    25081 acgatttcgc gcagatgacg ctggatttct ccaccgagtg ggtcgcctac caccgtgacg
    25141 gcgcgccgca gctgtacccc gaaccgctgc gcgccgagat cgaccaggtg agccggcgga
    25201 tctacaccga gatcaacaac ggtgtctacc gctgcggatt cgccggcact cagcaggcct
    25261 acgaagcggc ctacgaccgg ctgttcaccg cgttggattg ggtcagtgac cgcctgagaa
    25321 accagcgata cctggtgggc gacaccatca ccgaggcaga cgtgcggctc ttcaccacgc
    25381 tggctcgctt cgaccccgtc taccacgggc acttcaagtg caatcgaagc aaactcagcg
    25441 aaatgccggt gttgtggtcc tacgcgcgtg acctattcca gacgccgggt ttcggcgaca
    25501 ccgtcgattt cgtgcagatc aagcagcact actacatcgt gcacgccgac atcaacccaa
    25561 ccggaattgt gcccaagggc ccggacctgg ccgactggct gggtccacac ggccaggaag
    25621 cattgggagg caggcctttc ggagagggca ctccgccggg gccgccgcgc gacggcgagc
    25681 gcgtcccagc cgatcacggc gcgtagcgtc gtccggtggt tggttagaag gtcagccgca
    25741 ccgaccattc cgcgtgcgga acagcgcgga ccgtggtggt gtcactcggt tggctgtcgt
    25801 cgaccaccag cgtcagcaac tgcacgacta tcccggtcag ccgcccgcgc tgtgggtcga
    25861 ccgaggggat ggtgaccgcg aaccgcgtgc ccggccgaaa catcgtggtg gtggtgttgg
    25921 cggggtcctc atacacctgc agcaaacgcc acggtgcacg cgaaatggcc tcgggcaccg
    25981 agagttgcac cggaaagtct ccgttgaacg gaagctcgcc ctgggtctgg ggtgcctggc
    26041 agtcgttcag atcaagcaca ttgcaataga gatacggccc cacccgggtc aggtgcccgt
    26101 gcgaatacgc gctgatttcg ggttgctgtg gaccggcggg gcgtgcgagc agccacgctc
    26161 cgaccccggc ggccgcggcc agcagcagca ccaccaccgc gaggagcacg gccacaccgc
    26221 gtttcattcc ggcaccgccg ccggccgggc ggcgccccgc cgcagcccct cctgctcgac
    26281 catgacgggc cgattgcctc cgaggcccgg aatcaacgag tcgccgcgga agctgacgag
    26341 ggtctgcgcc aacccaagga tcagcagggc gctgaccgcg gtgaagccga cccacaattc
    26401 ggtgtagacc aagacgccca gcgccccgcc gagcacccac gccagctgca gcgtcgactc
    26461 cgagcgcccg aaccccgatg cccgcgactc ctcaggcaga tcgtgttgca acgaggcgtc
    26521 gagtgaggcc ttcgaaatgg cgcttgaccc cgaggtgacc agggtggcga tcacggccac
    26581 catgaggttg cccgccaccg acgccgccac cgcgaccgcg gacaccgcca ccgtgcagcg
    26641 caccaccagc acggccggcc tgcccagttg caggcgtgca ctggtgaaat tgccggcgaa
    26701 gttgccgatc gcggccgcgg cgccgatcac acccagcatg cccagctgga cccagccgtt
    26761 ggcctggtgt gccttggcga caaacgccgg gtacaagaac aggaagccca ccatcacctt
    26821 gatcgtgcaa ttaccccaca acgaggtgat gatgttgcgg cccagcggtt gtctcaatgt
    26881 tccgctgacc ttcttgacct cttcgggcca gtgccccagg cgcccccatc ggcgcggttg
    26941 ctcgctgtcg cggtggtagc tcaaggtggc cggcacttca ccctcggtga cctcgaccca
    27001 acgcggaatc cgcatcgaca gaaagacgcc gccgatcgtg atggcgacaa cgacgaacag
    27061 cgcgccgggc agcttgaaca ggtgggtgca gacgtactcg accccggcgg cgatggcgcc
    27121 ccccgcgatg gtgccgccaa gcaggccgaa tacggtcagc cgggaattga cccgcacgag
    27181 atcgatggtc ggcggcatca ccctcggtgt caccgcgctg cgcagcacgc tgaacgactt
    27241 tgagaacacc atcatcgcca gcgcgcacgg gtagaggacc atcgacggaa agctgccggc
    27301 ggcaccgtcg tagttcatga tcaacaccac cgccaacacg gtgcggagag cgaacgacag
    27361 cgccagcgcg actcgccggc catgctgcag ccgatccagc gccggaccga tgagcggcgc
    27421 gatcaccgcg aagggcgcaa tggtgatcaa caggtacagc gccactttgg acttgctctc
    27481 cccgctggcc gcggcgaaga acagggtgtt ggccagcgca acggtcattg cggagtcaac
    27541 cgcgaagttc gcgacgaccg gccacgtcag cgccgtcaga cccgatttgt cggcgccgtc
    27601 ggcggtggcg gcccgctgca ccatccagta catccgcgaa cccatctcgc ggcttcgctg
    27661 cgccgcggcg cgggtgacgg tgatgcgctc gccgggggcg gggccaggtg gctgcgcacc
    27721 gttaccgcgg tccggctccg gctcctggcc cagcggcggg agatagcggt tggcgctcgg
    27781 catcggcgat ggtcggcgcg ttcggcggta gtcagggtca tcggcagggt agttggccat
    27841 tcccggatgc tgggtggccg acccgttacg ggtatcgccg ctcgggtggg aggccacccg
    27901 agcgggatcc tcacgccgcc gtccagacac gaatcgattc tcccctatgg cgactccgtg
    27961 cgtctgcagg taacggggtg tggctcgcca acctagtatt ggaggcgcat tgcagagagg
    28021 ggacattgtg accgcaccca ccgaggaacc cgccgcgacg agcgtggccg agtcgcccgc
    28081 ggagttgacg gcggtgctca cgggtgcggt cggcctggcc agggccgccg tcgaggagtt
    28141 cagcggcctg gaggcggtcg gggaccatct gggcgtcagc ttcgaagatc cgaacgccgc
    28201 cacccaccgg ttcctggcgc atctgcccgg ctaccagggc tggcagtggg ccgtcgtcgt
    28261 cgccgcctac ccaggtactg agcacgtcac catcagcgaa gtggtgctca ttcccgggcc
    28321 gacggcgtta ttggcgcccg actgggtgcc ctgggagcaa cgtgtgcggc ctggggattt
    28381 gagtcctgga gacctgctgg cgccggcggc ggacgaccca cggttgctgc ccggctacac
    28441 cgccagtggc gacccgcagg tcgacgacgt cgccgccgaa atcgggttgg gccggcgctg
    28501 gctgctaagc gccctgggcc gcaccgaggc tgccgagcga tggcacaccg gggagtacgg
    28561 tcccgattcg gcgatggccc gctccaccaa acgcgtgtgc cgcgactgcg gttttttcct
    28621 gccgctggcc ggatcgctag gcgcgatgtt cggggtatgc ggcaacgagc tggccgccga
    28681 cgggcgtgtt gtggacaagc aatacggatg cggtgcccat tccgacactc cggcccctgc
    28741 ggggaccggg tcgccgattt acgagcccta cgacgacggc gtgctcgacg tcgtggagac
    28801 gtcatcggag tctctggtcg aatcagcgga tcttacggag tcccagcctg agccgcctga
    28861 atcgtcggag tctccgcccg agccggtgaa ctcgccggac tagcctttct cggcggcggc
    28921 cttgatgttt gccaacgagg tgttcatgcc gtcgagcaat tcgcgttcga agttcgcggt
    28981 cccacccaag agcgcattca ccccgaggct ggagattgcg ctgacgccgt tttcggcgtg
    29041 acgactctca atcagccgag tgccctggcc gttcggctcg agttcgtagc tccagatggt
    29101 gcggttggtg tcgacccgga acgccagctt cttctccggg atcacctcga cgacggtgca
    29161 ggtggtaggc cagaacaatc ggtggcggcg gttgaggttg acggtgcggg tgcccgcgcg
    29221 cagcggacct aagggcttca tccagcggca ttgcgggctc catgcgggca tccgccgaaa
    29281 atcggatatc aatgcccaga ctctcgacac cggtgcgttg atgtcgactt gggtttgcag
    29341 cagcggcgct accatttgct cctccgtgat tctgcgaaag ctatttgcgg tcgaggtagg
    29401 tttcgagtcc ggattgcgcg ccgcgcgcgc cgcggcgcgc agcggcaagc tgccacacga
    29461 agatgctggt accgatcagc ccgacgccca gcccgcccaa cgtcacggag cgccaactct
    29521 gcagggcagg tacgacgaac gcgagaaccg cgcagatcag ccagcccagg aagccgaccg
    29581 cgatgaaggg ccacacctcg agcagcgccg gcggcagggc cggcggcaca cggctctcgg
    29641 gaggctcttc agacatcccg atcaacatag cccggcgacg atgcaggccg ctgtgcggcc
    29701 tgaggtgggg gtaccgcccg cttgtggggg agaaggcggg cgattgaagt gagcccggcg
    29761 tccgtggaag cgttccggcg aaatttgccg cagctcggtg gaaggaccga agtcggtcgt
    29821 tcgccgtttc gtatcccgcg tttctcgggt tccgtgtaac ctcgcgccat gcctgacagc
    29881 gatgcgcggc tggccagcga cttgtcgttg gccgtcatgc ggctggcccg ccaactacgg
    29941 ttccgcaatc cgtcgtcgcc ggtgtcgttg tcgcagctgt cggcgctggc gacgctggcc
    30001 aacgagggcg ccatgacgcc cggcgcgctg gcgattcggg aacgcgtgcg gccgccgtcg
    30061 atgacccggg tgatcgcgtc gttggccgac atgggtctcg tggaccgcgc cccgcatccc
    30121 gtcgacggcc ggcaggtcct ggtgtccgtg tccgagtccg gcgccgaact ggtgaaggcc
    30181 aaccggcgcg ctcgtcagga gtggctcgcc gaacggctag tgacactgga cagcgagaaa
    30241 cgggaagtcc ttcgccaggc cgctgatctg atgttggcgc tggtcgacga aagcccgtga
    30301 gcgcacgtcg ctactccgac gtggatgtcc gggatgtcga cgatcccgac gatccgcggc
    30361 tcgacgactt ccgcgacctc aacagcgtcg atcgccgtcc tgacctgccc accggcaaag
    30421 ggctggtgat cgccgaaggt gtactggtcg tgcagcgcat gctcgcgtcc cggttcaccc
    30481 cgcacgcgct gctgggtacc gaccggcggc tggtcgagct caatgacgat ctggccggtg
    30541 ccaaagcgcc gttctaccga gcatcggccg atgtgatggc ccgggtggtc ggcttccacc
    30601 tcaatcgcgg cgtgctggcg gccgcgagaa gggtgccgga acccggagtt gccgagatgg
    30661 tcgagggcgc ccgtaccgtc gccgtgctcg aaggcgtcaa cgaccacgag aacctgggct
    30721 cgatcttccg caacgcggcg gggctggggg tggatgcggt ggtgttcggc agcggctgcg
    30781 ccgacccgct ctaccgtcgc gccgtccggg tatccatggg ccacgccttg ctggttcctt
    30841 acgcgcgtgc aaccaactgg cccgcggacc tcgtgctatt gaaagacaag ggatttcgac
    30901 ttctggcgat gacgccgcac agcgcggcgg gcacgctcgc ggaggcgatg gccgcggcgc
    30961 gtgacgaacg tatcgcggta gtgctcggtg ccgagggccc cggactcacg gaagtcgccc
    31021 tgcggctcag cgacatgcga gtgcgcgtcc cgatgtcgcg gggtaccgac tcgctcaacg
    31081 tcgcgacggc agccgcgttg gcgttctacg aacgggcccg cctcggcgac gatggcgccc
    31141 gcggcgggtc tccgcgcttg ccgggaaaca cggggaaggc gggctaggct cgcttcgtga
    31201 gcgaccagtc cgtgccctgg gcaacgggtt tgacggtgtc cgcgtttgtc gcggcggtca
    31261 ccggcgcggc ggtcgtggtg ctgactctcg ggctgatccg ggtgcatccg ctgttggccg
    31321 tcgggctgaa catcgtcgcg gtcggcgggc tggcgccgac gttgtggggc tggcggcgcg
    31381 t
//
LOCUS       DASSGQ010000227         3618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_532647,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000227 DASSGQ010000000
VERSION     DASSGQ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532647"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1944
                     /locus_tag="VFI55_09940"
     CDS             <1..1944
                     /locus_tag="VFI55_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HET7074384.1"
                     /translation="LSNISFGLNPAARQVVNSVFLHECQEAGLDSAIVHASKILPMNR
                     IPDQQREVALDLVYDRRREDYDPLQELMRLFEGVSAASSKESRAAELAKLPLFDRLAQ
                     RIVDGERNGLEADLDEAMTQKPPLQIINENLLAGMKTVGELFGSGQMQLPFVLQSAEV
                     MKTAVAYLEPHMEKSEDDSGKGRIVLATVKGDVHDIGKNLVDIILSNNGYEVVNLGIK
                     QPIANILEVAEDKSADVVGMSGLLVKSTVVMKENLQEMNTRGVAEKFPVLLGGAALTR
                     SYVENDLAEVYEGEVHYARDAFEGLKLMDTIMSAKRGVAVEPGSPEALAAEAARKKEA
                     ERKARHERSKRIAEQRKAAEEPVVVPERSDVAADVEVPVPPFWGSRIVKGLAVADYTG
                     LLDERALFLGQWGLRGVRGGEGPAYEDLVDTEGRPRLRYWLDRLSTDGVLAHAAVVYG
                     YFPAVSEGDDVVVLAEPKPDAPQRYRFTFPRQQRGRFLCIADFIRSRDLARERDEVDV
                     LPFQLVTMGQPIADFANELFASNSYRDYLEVHGIGVQLTEALAEYWHRRIREELTFSG
                     ERAMSAEDPEAVEDYFKLGYRGARFAFGYGACPDLEDRVKMMELLEPERIGVTLSEEL
                     QLHPEQSTDAFILHHPEAKYFNV"
     gene            complement(2080..3528)
                     /locus_tag="VFI55_09945"
     CDS             complement(2080..3528)
                     /locus_tag="VFI55_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPE family protein"
                     /protein_id="HET7074385.1"
                     /translation="MNAAAAPEAPMWFALPPEVHSTLLSTGAGPGPLLVAAEAWRALA
                     AQYASAAIELTGLLGAVQAAAWQGPTAARFVAAHQPFVCWLRQAGMVASATATGYETA
                     AAGYTSALAAMPTLVELAANHAVHGVLVATNFFGINTIPIALNEADYVRMWIQAAATM
                     SVYQAVSGESLAATPTTSPAPQIVTTDATPAADSTFPDPTKLILQLFDEFLSTLRAMA
                     FEYLPGPVGGLVSQVLDSFIAFASGEVFTILAYSVLDPLIYFGPFTPALSPFLSPIGL
                     VGLAGIAGVDPASMSPAAGAHSPSPGQQGWPAVSEVATAGAGPATPAAAAGPLAPSAA
                     SAAPSVPAGAAPGFYAVGGDPGGEGFTPTSGTRAAAAVAAGSAAPAVANLAASERARA
                     TRKAKARQQVRKFRFEYLEDDARITPSDDPVTGAVAASESGSGTLGFTGTLSKSAAAQ
                     AKGLIRLDNGAFGEPTQEPMLPNTWDAGRKAE"
BASE COUNT          620 a         1125 c         1292 g          581 t
ORIGIN      
        1 ttgtccaaca tctccttcgg cctcaatccc gccgcgcgtc aagtagtcaa ctccgtgttc
       61 ctgcacgaat gtcaggaagc cggtctggac tcggcgatcg tgcacgcgtc caagatcctg
      121 ccgatgaacc ggataccgga ccagcagcgc gaggtcgcgc tggatttggt ctacgaccgc
      181 cgccgcgagg actatgaccc gctgcaggag ctgatgcggc tcttcgaagg cgtgtcggcg
      241 gcgtcgtcga aggagtcgcg cgcggccgag ctggccaaac tgccgctgtt cgaccggctg
      301 gcccagcgca tcgtcgacgg cgagcgcaac ggcttggagg ccgacctgga cgaggcgatg
      361 acccagaagc cgccgctgca aatcatcaac gagaacctgt tggccgggat gaagacggtc
      421 ggtgagctgt tcggctccgg gcagatgcag ctgccgttcg tgctgcagtc cgccgaggtg
      481 atgaagaccg ccgtcgccta cctggaaccg cacatggaga aatccgagga tgactccggc
      541 aagggccgca tcgtgctggc caccgtcaag ggcgacgtgc acgacatcgg caagaacctg
      601 gtcgacatca tcttgagtaa caacggctac gaggtggtca acctcggcat caagcagccg
      661 atcgccaaca tcctcgaggt cgccgaagac aagagcgccg acgtggtcgg gatgtcgggc
      721 ctgctggtga agtcgaccgt ggtgatgaag gaaaacctcc aggagatgaa tacccgcggg
      781 gtggccgaaa agtttccggt gctgctcggc ggtgcggcgc tgacccgcag ctacgtggag
      841 aacgacctcg ccgaagtgta cgagggcgaa gtgcattacg cgcgagacgc tttcgagggc
      901 ctgaagctga tggacaccat catgagcgcc aagcggggcg tggcggtgga gccggggagt
      961 ccggaggcct tggcggccga agcggcccgc aagaaggaag ccgagcgcaa agcccgccac
     1021 gagcggtcca agcgcatcgc ggagcagcgg aaggcggccg aggaacccgt cgtggtgccc
     1081 gaacgctcgg atgtcgcggc cgacgtcgag gtgcccgtcc cgccgttctg gggttcgcgc
     1141 atcgtcaagg gactggcggt cgccgactac accggactgc tggacgagcg cgcattgttc
     1201 ttggggcagt ggggattacg cggtgtgcgt ggcggcgaag gacccgcgta cgaggacttg
     1261 gtggacaccg agggccgacc gcggctgcgg tattggctgg accgcttgtc caccgacggc
     1321 gtcttggcgc acgccgcggt ggtgtacggc tacttcccgg cggtgtccga aggcgacgat
     1381 gtggtcgtgc tcgccgagcc caaacccgac gccccgcagc gctaccggtt taccttcccg
     1441 cgccaacagc gcggccggtt cctgtgcatc gccgacttca tccggtcccg ggatctcgcc
     1501 cgggagcgcg acgaggttga cgtgctgccg ttccagctgg tgacgatggg ccaacccatc
     1561 gccgacttcg ccaacgagct gttcgcgtcg aactcctacc gtgactacct cgaagtgcac
     1621 ggcatcggcg tgcagctcac cgaggcgctg gccgagtact ggcaccggcg aattcgcgag
     1681 gagctcacgt tctccggcga gcgggcgatg tcggccgagg atcccgaggc ggtcgaagac
     1741 tatttcaagc tcggttaccg cggcgcgcgc ttcgccttcg gttacggggc ctgcccggac
     1801 ctcgaggacc gggtgaagat gatggagcta ctggagccag agcgcatcgg cgtgacgttg
     1861 tcggaggagt tacagctgca ccccgagcag tcgaccgacg ccttcatcct gcaccacccc
     1921 gaggccaagt acttcaacgt ctagaagcgc gccgctggag agccctgaag ccgtcgcgag
     1981 tgtgcgtgtc tgcacaacga cacgccgctg atcccggcaa tctgtgcacg ctcgcgggcg
     2041 gggatcaatc cagctggctc ccgcctgcgg gttgtccgcc tattccgcct ttcggccggc
     2101 atcccaggtg ttgggcagca tgggctcttg ggtgggctcg ccgaatgcgc cgttgtcgag
     2161 gcgaataagt cccttcgcct gcgcggcagc cgatttcgac aatgttccgg tgaatccgag
     2221 tgttcctgat ccgctttcgg aggcggcgac ggccccggtc accgggtcgt ccgacggggt
     2281 gatgcgagcg tcgtcttcca ggtactcgaa ccggaacttc cggacctgct ggcgcgcctt
     2341 cgctttccgc gttgctctgg cccgctcact ggctgccagg ttggcgaccg cgggtgccgc
     2401 gctgcccgcg gcgaccgcgg ccgcggctct ggtgccggac gttggggtga agccttcacc
     2461 gccggggtcg cccccgaccg cgtaaaagcc tggtgcggcg ccggccggga cggaaggcgc
     2521 cgccgaagcg gcggatgggg ccagtgggcc ggccgcggcg gcgggcgtag cggggccggc
     2581 gcccgccgtc gcgacctcgg aaaccgcggg ccaaccctgc tggccgggcg aaggtgagtg
     2641 cgcgcccgcg gccggcgaca tcgaagctgg atcgactccg gcgatgcccg cgagcccgac
     2701 caagccgata ggcgacagga aagggctcag cgcgggcgtg aacggtccga aatagatcaa
     2761 ggggtccagc accgagtagg cgaggatggt gaagacttcg ccggacgcga acgcaatgaa
     2821 cgagtccagc acctgggaga ccaggccgcc gactggcccg ggcagatatt caaaggccat
     2881 cgcgcgcagg gtgctgagga actcgtcgaa tagctgaagg atcaacttgg ttgggtccgg
     2941 gaaggtgctg tcggcggcag gggtggcatc agtggtgacg atctgcggtg cgggcgacgt
     3001 cgtcggggtc gccgcgaggc tttccccgga gacggcctgg tagacgctca tcgtggcggc
     3061 ggcctggatc cacatgcgga cgtagtcggc ttcgttgagc gcgatgggga tcgtgttgat
     3121 gccgaaaaaa ttggtggcca ccagaacgcc gtgcacggcg tggttggcgg cgagctcaac
     3181 cagtgtgggc atggcggcca atgcggatgt gtacccagca gctgcagttt cgtatcccgt
     3241 ggcggttgcg ctcgccacca tgccggcctg gcgcagccag cagacaaagg gttggtgagc
     3301 ggcgacgaac cgcgctgccg tcggcccctg ccatgcggcg gcctgtaccg cgccgagaag
     3361 tccggtgagt tcgattgccg cggaggcgta ttgggcggcc agtgcccgcc atgcttcggc
     3421 ggcgaccagg agcggaccgg gaccggcgcc tgtggacagc agcgtggaat gcacctcggg
     3481 gggaagcgcg aaccacatcg gagcttcggg cgctgcagca gcgttcactg ttgaccggtc
     3541 cttcgcggca ggagcacagc tattaggtta ggctaaacta gcttagtatg gcctaacttg
     3601 cggcgcgtcg gcacgaaa
//
LOCUS       DASSGQ010000228         6320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_897067,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000228 DASSGQ010000000
VERSION     DASSGQ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897067"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..755)
                     /locus_tag="VFI55_09950"
     CDS             complement(18..755)
                     /locus_tag="VFI55_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7074386.1"
                     /translation="MWYWLFKYILLGPLLHLLGRPKVEGLEHVPSSGPAILASNHLAV
                     MDSFYLPLVVRRRITFLAKAEYFTGTGLKGWFSRWFFTAAGQVPIDRADADAAQAALN
                     TAQRLLGEGKLLGMYPEGTRSPDGRLYKGKTGLARLALETGVPVIPVAMVGTDVVNPP
                     GTTMLRFGRVTVRFGKPMDFSRFDGLAGNRFIERAVTDEVIYELMGLSGQEYVDIYAA
                     SIKERSNGSTGPGPTDEATRIPETAAG"
     gene            complement(839..1234)
                     /locus_tag="VFI55_09955"
     CDS             complement(839..1234)
                     /locus_tag="VFI55_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074387.1"
                     /translation="MSPAHPEIGPELRKLAQAVLDAIDPVVKTAAALTAGAGLGTGKC
                     QQVWCPVCAMAALATGEQHPLLTIIADHSIALLDAIRGIVDDIDRSPELPPDGPPDGG
                     ITAGPDVETIATNGAAKTRYEPISLTVEE"
     gene            complement(1231..2505)
                     /locus_tag="VFI55_09960"
     CDS             complement(1231..2505)
                     /locus_tag="VFI55_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003914048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HET7074388.1"
                     /translation="MSESFSPIPARISLFVGKGGVGKSTLACATAVCDAAVGQRVLLV
                     STDQAHSLGDVLGVAVPASGLGGAVRVPVDLETGQAEATGGFLDALALDTLALLEARW
                     LDVVNTLDRRFPESELSSIAPEELSALPGVQEVLGLHAVGELAESGRWDRIVVDCAST
                     ADALRMLTLPATFGLYVERAWPRHRRLSIGADDTRSAAVVELLERINAGVDRLSALLT
                     DGELVSAHLVLTPERVVAAEAVRTLGALALMGVRVEELIVNQVLQADASYEYRNLPDH
                     PAFYWYAERISEQRVVIQELDATMGDVALVLIPHLSGEPIGPKALGALFDSAGRRGGA
                     EPPGPVRPVVDLESGSGLGSIYRMRLTLPQLDPAGLTLGRVDDDLIVSAGGLRRRVRL
                     ASVLRRCTVLDAHLRGCELTVRFQPDPEVWPK"
     gene            complement(2498..2935)
                     /locus_tag="VFI55_09965"
     CDS             complement(2498..2935)
                     /locus_tag="VFI55_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7074389.1"
                     /translation="MAEKTTQTIHIEADPGTVLKVIADIDSYPEWISEYKEAEVQERG
                     ADGYPKVVRFVMDAGVIKDTLVMSYQWPADRKSLSWTLVSSSLLRSLEGTYRLAPSGS
                     GTDVTYELSVDLAIPMIGLLKRKAERRLTDTALKDLKKRVEAE"
     gene            complement(3051..3440)
                     /locus_tag="VFI55_09970"
     CDS             complement(3051..3440)
                     /locus_tag="VFI55_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase / dehydrase and lipid
                     transport"
                     /protein_id="HET7074390.1"
                     /translation="MNSIQIADQTYVAADGARVGAAVADESCWRRWWPDLRLQVIEDR
                     ADKGIRWAVTGALTGTMEIWLEPSMGGVVLHYFLHAEPTGAAAWQLAKLNLAKMTHRR
                     RVAGKKMAFEVKRTAERSRPVGVSPVV"
     gene            3680..5485
                     /locus_tag="VFI55_09975"
     CDS             3680..5485
                     /locus_tag="VFI55_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET7074391.1"
                     /translation="MREYSVPARFAVGERDNIAAMVFEHERDDPDFVIFQRQVDDAWT
                     DVTCAEAAEQVRSAALGLIALGVQAGDRVSIFSATRYEWAIFDLAILSVGAITVPIYE
                     TSSAEQVRWVLQDSEAVLAFAETDAHASMVTELTAELPAIRRVLHIDGSGPKALDQLV
                     EAGATVDKAELTARLEALRAEDPATLIYTSGTTGRPKGCQLTHSNLLYETRGSKECLP
                     TLLNEGQRLLIFLPLAHVLARSLTLSAYANKVTVGFTSDIKNLLPMFAVFKPTVVVSV
                     PRVFEKVYNTAEQNAANDGKGQIFAAAVQTAVDWSQAQDRGGPGLLLRAKHAVFDRLV
                     YHKLRAALGGDCHASVSGGAPLGARLGHFYRGVGLTIHEGYGLTETSSAITVNQVGRV
                     NIGTVGTLLPGNSMRIADDGELLVRGGVVFGGYWRNAQATEEAFADGWFKTGDLGSLN
                     EDGFLKITGRKKEIIVTAGGKNVAPAVLEDQLRAHPLISQAMVVGDAKPFVGALITID
                     PEAFDAWKARNQKQAGASVGDLVNDPDLVAEVDAAVKRANLSVSSAESIRKFRILPVD
                     FTEDTGELTPTLKVKRNVVAEKFASDIESIYEKDE"
     gene            complement(5501..>6320)
                     /gene="pimB"
                     /locus_tag="VFI55_09980"
     CDS             complement(5501..>6320)
                     /gene="pimB"
                     /locus_tag="VFI55_09980"
                     /EC_number="2.4.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose-dependent
                     alpha-(1-6)-phosphatidylinositol monomannoside
                     mannosyltransferase"
                     /protein_id="HET7074392.1"
                     /translation="AGATRVLASTHGHEVGWSMLPGARSVLRRIGDSTDVVTFVSRYT
                     RSRFASAFGPDARLEYLPPGVNADRFRPDPAARAELRARYRLGDRPTVVCLSRLVPRK
                     GQDVLIKALPSIRRRVDGAALVIVGGGPYLEALRNLTAECGVADDVTFTEGVPDGELP
                     AHHALADVFAMPCRTRGAGMDVEGLGIVFLEASATGVPVIAGLSGGAPETVQDNKTGL
                     VVDGRSVSAVADAVAGLLTDRDRAEAMGVAGREWVTSQWRWDTMAARLADLLRG"
BASE COUNT         1104 a         2206 c         2026 g          984 t
ORIGIN      
        1 aacggtgata acgggcgcta cccggcggcc gtttccggga tccgcgtagc ctcgtcggtc
       61 ggccccgggc cggtcgagcc gttgctgcgt tccttgatgc tggcggcgta gatatcgacg
      121 tactcctggc cggacaggcc catcagctcg tagatcacct cgtcggtgac ggcccgctcg
      181 atgaagcggt tgccggccag cccgtcgaag cgtgagaagt ccatcggctt gccgaaccgg
      241 acggtgacgc ggccgaaccg caacatcgtg gtccccggcg gattgacgac gtcggtgccg
      301 accatcgcca ccggaatcac cggcaccccg gtctcgagcg ccagccgcgc cagcccggtc
      361 ttccccttgt acagccggcc atcgggcgac cgggtgccct cggggtacat ccccagcagt
      421 ttgccctcgc ccagcagccg ctgggcggtg ttaagcgcgg cctgagcggc gtcggcgtcg
      481 gcgcggtcga tcggcacctg gccggcggcg gtgaagaacc agcggctgaa ccagcccttg
      541 agcccggtgc cggtgaaata ttcggccttg gcaaggaaag tgattcgccg tcgcaccacc
      601 agaggcaggt agaagctgtc catcaccgcg aggtggttac tggccaggat cgccggtccg
      661 gaactcggaa catgttccag cccttcgact ttcggtctac cgagcaggtg cagcagcggg
      721 cccagaagga tgtacttgaa tagccaatac cacatggccc tccctctcac ccgcaccgta
      781 cgcgctctgc caactgtacc gatccgcagc ggcagggacc acctcccgcg caggccggtc
      841 actcctcgac ggtcagcgaa atgggctcgt aacgggtctt cgcggcgccg ttggttgcga
      901 tggtctcgac gtccggccca gccgtgatgc cgccatcggg cgggccgtcc ggtggcagtt
      961 cgggcgaccg gtcgatgtcg tcgacgatgc cccggatcgc gtccagcaaa gcgatgctgt
     1021 ggtcggcgat gatggtgagc aacgggtgtt gctcaccggt tgccaacgcg gccatcgcgc
     1081 ataccggaca ccacacctgc tgacacttgc cggttccgag tccggcgccg gccgtcaagg
     1141 cggccgccgt tttcaccacg ggatcgattg cgtccagcac cgcctgggca agtttccgca
     1201 gttcaggacc aatttctgga tgagcgggac tcacttgggc cacacctccg gatccggttg
     1261 aaaacgaact gtcagctcac aaccccgtag gtgtgcgtca agcacagtac atcgccgcag
     1321 cacggatgcc aaccgaactc tgcgccgcaa cccgccggcg ctgacgatca ggtcgtcgtc
     1381 gacccgcccc agcgtcagtc ccgcgggatc gagctggggc aacgttagtc gcatgcggta
     1441 aatcgaccca agtcccgagc cggattcgag gtcgacgacg ggacgcaccg gacccggcgg
     1501 ctccgccccg ccccgccggc cggcgctgtc gaacagcgcg cccagcgcct tgggaccgat
     1561 cggctcgccg gacaggtgcg ggatcaacac cagcgccacg tcccccatgg tggcgtccag
     1621 ctcctggatg acaacgcgct gttcggagat gcgttcggcg taccagtaga acgcggggtg
     1681 gtcgggcagg ttgcgatact cgtaggacgc gtctgcctgc agaacctggt tgacgatcag
     1741 ctcttcgacc cgcacaccca tcagcgccaa cgcacccagc gtccggaccg cctcggccgc
     1801 aaccacccgc tcgggggtca gcaccaggtg cgcactgacc agctccccgt cggtcagcaa
     1861 cgcgctgagc cggtcgacgc ccgcgttgat gcgctcgagc agttccacca ccgcggccga
     1921 gcgggtgtcg tcggcgccga tggacagcct gcggtggcgc ggccacgcgc gctcgacgta
     1981 gaggccgaag gtggcgggca acgtcagcat gcgcaacgca tccgcggtcg aggcgcaatc
     2041 gacgacgata cgatcccatc gcccggactc ggcgagctca ccgacggcgt gcagaccaag
     2101 cacttcctga acgcccggca atgcggagag ttcttcggga gcaatggagc tcaactcgga
     2161 ctcgggaaac cgccgatcca atgtgttgac cacgtcaagc cagcgggcct cgagcagggc
     2221 cagggtgtcc agcgcgagcg cgtcgaggaa gccaccggtc gcctccgcct gcccggtttc
     2281 gaggtcgaca ggcacccgaa ccgctccgcc cagtccgctc gccggcacgg ccacacccag
     2341 tacgtcgccc agcgaatggg cctggtcggt ggacaccagc agcacccgct gcccgacggc
     2401 cgcatcacag accgcggttg cgcacgccag ggtggatttc cctaccccgc ccttgccgac
     2461 gaagagactg atccgggccg gaatgggcga aaaggattca ctcagcctcg actcgtttct
     2521 tcagatcctt caacgcggtg tcggtcagcc tgcgctcggc cttgcgcttg agcaacccga
     2581 tcatcgggat ggcaagatcc accgagagtt cgtaggtgac gtcagtgcca gatccgctgg
     2641 gcgccaggcg atatgtgccc tcaagtgacc gcagcagcga gctggagacc agcgtccagc
     2701 tcagcgattt acggtccgcc ggccactgat aggacatcac cagggtgtct ttgatgacgc
     2761 cggcgtccat cacgaatcgc acaaccttcg ggtagccgtc ggcgcctctt tcctgcacct
     2821 cggcttcctt atattccgag atccattccg ggtaggaatc gatatcggcg atcaccttca
     2881 gcaccgtgcc cggatcggct tcgatgtgga tggtctgcgt cgtcttctcc gccacctggc
     2941 tgctgccctc tctcccacaa acgggtaggc cccgcgatgt gcatgactac ccaggacgat
     3001 cttggcctcc gggccgaaac gctactctcc gtgccaaacc ctgatcgcag ttaaactacc
     3061 ggagaaacgc ctaccggtcg tgaccgttcc gccgtcctct tcacctcaaa ggccattttc
     3121 ttccctgcca ctcgacgccg gtgcgtcatt ttcgccaggt tgagcttggc cagctgccaa
     3181 gccgccgcgc cggtgggctc ggcgtgcaaa aagtagtgca gcaccacccc gcccatagat
     3241 ggttccaacc agatctccat ggtgcccgtc aacgcgccgg tgaccgccca gcggatcccc
     3301 ttatcggcgc gatcctcgat cacctgcagc cgcaagtccg gccaccaccg acgccagcag
     3361 gactcgtcgg cgaccgcggc accgacccgt gcgccgtcag cggcgacata cgtctggtcc
     3421 gcgatctgga tgctgttcat cagctcagct tcacacacca atctgaagcg cccacgtgag
     3481 ggcgcggcgg agccccaccg acgagccgaa tgcctgccga ccgccgcgct gtcccgcgtc
     3541 tgccgcaaag gtggcggtgc cccgaccgac ccccgccatg ctgcgcgact gctcagaata
     3601 ggctgggcac agcaagccgg cctgtagcgg gtcgcgcaac gtcaccaagt caaaaagtca
     3661 ccaagtcaaa gaggtcaaag tgcgtgagta cagtgtcccc gcccgcttcg ccgtcggcga
     3721 gcgggacaac atcgcggcca tggtcttcga gcacgagcgc gacgatcccg acttcgtcat
     3781 ctttcaacgt caggtcgacg acgcgtggac cgatgtcacg tgcgccgagg ccgccgagca
     3841 ggttcggtcc gcggcactgg gcctgatcgc cctgggggtg caggccggtg accgggtgtc
     3901 catcttttcg gcgacccgct acgagtgggc gatcttcgac ctcgcgatcc tgtcggtggg
     3961 agcgatcacc gtgccgatct acgagacgtc ctcggccgag caggtgcgct gggtgctgca
     4021 ggactcagag gcggtgttgg cattcgccga aaccgacgca cacgcctcga tggtcaccga
     4081 actcaccgcc gagctgcccg cgatccgccg ggtcctgcac atcgacggct cgggccccaa
     4141 agcgctcgac cagctggtcg aggccggtgc gacggttgac aaggccgagc tgaccgcgcg
     4201 ccttgaggcg ctgcgcgccg aggatcccgc gacgctcatc tacacctcgg gcaccaccgg
     4261 acgacccaag ggctgccaac tcacccattc caacctgctt tacgagaccc gcggcagtaa
     4321 ggaatgcctg ccgacgctgt tgaacgaagg gcagcggcta ctgatcttcc tgccactggc
     4381 gcacgtgctg gcccggtcgc tcaccttgtc cgcatacgcc aacaaggtga ccgtcgggtt
     4441 caccagcgac atcaagaacc tgctgccgat gttcgcggtg ttcaaaccga cggtggtggt
     4501 gtcggtgccg cgggtgttcg agaaggtcta caacacggcc gagcaaaacg ccgccaacga
     4561 cggcaagggg cagatcttcg ctgcggccgt gcaaaccgcg gtcgactgga gtcaggccca
     4621 agaccgcggc ggacccgggc tgctgttgcg cgccaagcat gcggtattcg accggctggt
     4681 ctaccacaag ctgcgcgcgg cgctgggcgg cgactgccac gcgtccgtct cgggtggcgc
     4741 accgttgggc gcgcgactgg gccacttcta ccgcggtgtc ggcctgacca tccacgaggg
     4801 ctacggcctt accgagacca gctccgccat cacggtcaac caggtcggca gggtcaatat
     4861 cgggaccgtt ggaacgttac tgcccggcaa cagcatgcgc atcgccgacg acggtgagct
     4921 gctggtgcgc ggcggtgtgg tgttcggcgg ctactggcgc aacgcgcagg ccaccgagga
     4981 ggcattcgcc gacggctggt tcaagaccgg tgacctgggc tcgctcaacg aggacggctt
     5041 cttgaagatc accggccgca agaaggaaat catcgtcacc gccgggggca agaacgtcgc
     5101 cccggccgtg ctcgaggacc agctgcgggc gcacccgctg atcagccagg ccatggtggt
     5161 gggggacgcc aagccgttcg tcggcgcgct gatcaccatc gaccccgagg cgttcgacgc
     5221 ctggaaggcg cgcaaccaga agcaggccgg cgcgtcagtc ggcgatctgg tcaacgaccc
     5281 cgacctggtc gccgaggtgg acgcggccgt caagcgcgcg aacctgtcgg tgtcgagcgc
     5341 cgagtcgatc cgcaagttcc gcattctgcc ggtcgacttc accgaggaca ccggtgagct
     5401 gacaccgact ttgaaggtga agcgcaacgt ggtggccgag aagttcgcct ccgatatcga
     5461 atcgatctac gagaaagacg agtagccgcc cgcgggcccg tcagccacgc agaaggtcgg
     5521 ccagccgggc ggccatcgtg tcccagcgcc actgggacgt cacccactcg cggccggcga
     5581 cgcccattgc ctccgcacgg tcgcgatcgg tcagcaaccc ggcgacggcg tcggcgaccg
     5641 cgctcaccga gcggccgtcg acaacgagcc cggtcttgtt gtcctgcacc gtttctggag
     5701 ccccaccgga caggccggcg atgaccggca cgccggtcgc ggaggcctcc aggaacacga
     5761 tgcccagccc ctcgacgtcc atgccggcgc cgcgggtgcg gcacggcatc gcgaacacgt
     5821 cggccagggc gtgatgggcg ggcaattcgc cgtccggcac accctcggtg aacgtcacgt
     5881 catcggccac gccgcattcc gcggtcaggt tgcgcagcgc ttccagatag ggaccgccac
     5941 cgacgatgac caacgcggct ccgtcgacgc gacgccggat cgatggcagc gccttgatca
     6001 gcacgtcctg ccctttgcgc ggcaccagcc gcgacaggca caccaccgtg ggccgatcgc
     6061 cgagccggta gcgagcgcgc agttcggccc gggcggccgg atcgggccgg aaccggtcgg
     6121 cgttcacccc ggggggcagg tattccaacc tggcgtcggg accgaacgcc gacgcgaacc
     6181 gggaccgcgt gtagcggctg acgaaagtga cgacgtcggt actgtcgccg atgcgacgca
     6241 ataccgaccg cgcaccggga agcatcgacc agcccacttc gtggccgtgg gtgctggcca
     6301 gcacgcgggt tgccccggcc
//
LOCUS       DASSGQ010000229         4149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_623519,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000229 DASSGQ010000000
VERSION     DASSGQ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623519"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VFI55_09985"
     CDS             <1..769
                     /locus_tag="VFI55_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET7074393.1"
                     /translation="GAPVPHILVADDSLAALGNPFLICDEIKGETIVRRIQRQLDAAD
                     GQAGRARLLRQCAQALAAIHRADSHDPGLVHEDQLLQWRERLDEMGDTTATFEWAFRW
                     LEANRPRKSPQSRPSAAVLVHGDYRMGNLIVDGSDLAAVLDWELVHVGEACEDLAWFC
                     IRAWRFGAPPGLRAGGLGSIESFVRAYEEAGATTVDRMALHWWLVLATLRWGVICRHQ
                     AERHLSGQIRSVELATIGRRVCETEWDLLELLEKAAP"
     gene            766..1116
                     /locus_tag="VFI55_09990"
     CDS             766..1116
                     /locus_tag="VFI55_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HET7074394.1"
                     /translation="MTGGYGRPTAAELVAAVADFLETDIREATSGQVNFHARVAANAL
                     RMVERELLDEGEAQCRAALADLGFANEEALAGAIRAGEMDGRANDVTACLRTLVRHRL
                     AVAHPGYDSELTPN"
     gene            1138..1674
                     /locus_tag="VFI55_09995"
     CDS             1138..1674
                     /locus_tag="VFI55_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET7074395.1"
                     /translation="MTTLEALLTDPDMTGVWNLVPARSTITFTVKNMWGLANVKGRFT
                     EFSGDAQLTGKGAVFGRVDIRAASLSTGIRARDKHLRSADFFDVERCPEISLVVTAVH
                     PTRGNIADLRANVSIKGITAPLPLPVTITEFADGSIQISGETKVDRSQFDLGWNRFGM
                     IAATATAAAQAVFARSPR"
     gene            1704..2105
                     /locus_tag="VFI55_10000"
     CDS             1704..2105
                     /locus_tag="VFI55_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074396.1"
                     /translation="MPDSSSALRILVYSDNARTREQVMQALGKRLHPSLPELSYIEVA
                     TGPMVIRTMDEGGIDLAILDGEATPTGGMGIAKQLKDELETCPPVLVLTGRPDDAWLA
                     NWSRAEAAVPHPLDPIALGHAALGLLRAPAR"
     gene            2286..2663
                     /locus_tag="VFI55_10005"
     CDS             2286..2663
                     /locus_tag="VFI55_10005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET7074397.1"
                     /translation="MNVYIPILVLGAIAAAFAVGSVVIASLVGPSRYNKSKVAAYECG
                     IEPTEMPVSGPHATSGQRFPVKYYLTAMLFIVFDIEIVFLYPWAVSYDALGTFALVEM
                     VVFMLTVFVAYAYVWRRGGLTWD"
     gene            2672..3226
                     /locus_tag="VFI55_10010"
     CDS             2672..3226
                     /locus_tag="VFI55_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HET7074398.1"
                     /translation="MGLEEQLPGGILLSTVEKVAGYVRKNSLWPATFGLACCAIEMMA
                     TAGPRFDIARFGMERFSATPRQADLMIVAGRVSQKMAPVLRQIYDQMAEPKWVLAMGV
                     CASSGGMFNNYAIVQGVDHLVPVDIYLPGCPPRPEMLLHAILKLHEKIQEMPLGVNRE
                     HAIAEAERAALAARPTIEMHGLLR"
     gene            3223..3930
                     /locus_tag="VFI55_10015"
     CDS             3223..3930
                     /locus_tag="VFI55_10015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET7074399.1"
                     /translation="MSSPSQDPREAVTGPDDEVIDVRRGMFGVSGTGDTSGYGRLVRE
                     VALPGSSSRPYGGYFDDIADRLAEALRRDDVEFDDAVEKVVVDRDELTLHICRDLLPQ
                     VAQRLRDEPDLRFELCLGVSGVHYPHETGRELHAVYPLQSITHGRRLRLEVAAPDGDP
                     HIPSLFSVYPTNDWHERETYDFFGIIFDGHPSLTRIEMPDDWQGHPQRKDYPLGGIPV
                     EYKGAQIPPPDERRGYN"
     gene            3930..>4149
                     /locus_tag="VFI55_10020"
     CDS             3930..>4149
                     /locus_tag="VFI55_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit D"
                     /protein_id="HET7074400.1"
                     /translation="MSDTTATAYGDDAAETVLVAGGQDWEQIIDAARNSDPGERIVVN
                     MGPQQPSTYGVLRLILEIEGETVISARCG"
BASE COUNT          718 a         1381 c         1397 g          653 t
ORIGIN      
        1 cggagcgccg gtcccacaca tcctggtcgc cgacgattcg cttgccgcac tgggcaatcc
       61 gtttctgatc tgcgacgaga tcaagggcga gaccatcgtt aggcgcatcc aacgtcagct
      121 cgacgctgcc gatgggcaag cgggtcgggc gcggttgctg cggcagtgtg cgcaggcgct
      181 ggccgccatc caccgggccg actcgcacga tcccggcctg gtgcacgagg accaactcct
      241 gcagtggcgc gagcggctcg acgaaatggg cgacaccacc gccaccttcg aatgggcgtt
      301 ccgctggctg gaggccaacc ggcctcggaa atccccgcag tcccggccct cggcggcggt
      361 cctggtacac ggcgactacc ggatgggaaa cctgatcgtc gacggatccg acctggcggc
      421 cgttctggac tgggaactgg ttcacgtggg cgaggcatgc gaagacctgg cctggttctg
      481 catccgggcg tggcggttcg gcgccccgcc cggcctgcgg gccggcggcc tcggcagcat
      541 cgagagcttc gtgcgggcct atgaggaggc cggcgcgact accgtggatc gcatggcgct
      601 gcactggtgg ctggtgctgg cgacgctgcg gtggggcgtc atctgccgtc atcaggcgga
      661 acggcatctg agcggccaaa tccgttcggt ggagttggcg accatcggcc gccgcgtctg
      721 cgagaccgag tgggacctgc tcgagctcct cgagaaggcg gcgccgtgac cggcgggtac
      781 ggtcgcccga ccgcagcgga actggtggcc gcggtggccg acttcctgga aaccgacatc
      841 cgggaggcga ccagcggaca ggtcaacttc cacgccaggg tggcggccaa cgcgttgcgc
      901 atggtcgagc gcgaactgct cgacgaaggg gaggcgcagt gccgcgccgc actggccgac
      961 ctgggtttcg ccaacgagga agcgctcgcc ggcgccatcc gcgccggcga gatggacggg
     1021 cgcgccaacg atgtgaccgc ctgcctgcgc acgctggtgc gccaccggct cgcggttgcc
     1081 caccccggat acgacagcga actgacaccg aactgacacc gaactgaaga ggtgaccatg
     1141 acgactctgg aagcactgct gaccgatccc gacatgaccg gcgtgtggaa cctggttccc
     1201 gcccgctcga ccatcacctt caccgtcaag aacatgtggg gcctggcgaa tgtcaagggc
     1261 cgcttcaccg agttcagcgg cgacgcgcaa ctgaccggca aaggcgcggt tttcggccgc
     1321 gtcgacatcc gggccgcgtc gctgagcacc ggcatccgcg ctcgcgacaa gcacctgcgc
     1381 tcggccgact tcttcgacgt ggagcgatgc cccgagatca gcctcgtcgt caccgcggtg
     1441 cacccgaccc ggggcaacat tgccgacctg cgggccaacg tcagcatcaa aggcatcacc
     1501 gcgccgctac ctctgcccgt cacgatcacc gagttcgctg acgggtccat ccagatctcc
     1561 ggcgaaacca aggtcgaccg gtcccaattc gatctgggct ggaaccggtt cggcatgatc
     1621 gcggcgacgg cgacggcggc tgcgcaggcc gtctttgctc ggtcaccccg gtaaggtcgg
     1681 cccgtcacca gtaccatctg accgtgcccg actccagcag cgccctgcgg atcctcgtct
     1741 acagcgacaa cgccagaacc cgcgaacaag tgatgcaggc actgggcaag cgcctgcacc
     1801 cgtctctgcc ggaattgagc tacatcgagg tggcaaccgg cccgatggtg atccgcacca
     1861 tggacgaggg tggcatcgac ctggccattc tcgacgggga ggcgaccccg accggtggca
     1921 tgggaatcgc caaacagctc aaggacgagc ttgagacctg cccgccggtg ctggtgctca
     1981 ccggccgccc ggacgatgcg tggctggcca actggtcgcg ggccgaagcc gcggtgccgc
     2041 atccgctcga ccccatcgcg ctgggccacg cggcgctcgg cctgctgcgc gcccccgccc
     2101 gctaggctgg cgcgccaaac cgcgccacca gcacccaccc cggacatgtg cgccgaccgt
     2161 cacgacgcta tgttgaaggt cactgtgacg ggctacagac ccggctcgtc acggcagccc
     2221 ggtcaccgcc cggccgccac tcagcggcct tagaaggcgg cccatacgac ggatcatgga
     2281 gcgagttgaa cgtctacatt cccatcctgg tactgggggc gattgccgcc gccttcgccg
     2341 tgggctcggt tgtcatcgcg agcctggtcg gcccgtcgcg gtacaacaaa tcgaaggtgg
     2401 cggcctacga atgtggcatc gagcccaccg aaatgccggt gagcggcccg cacgcgacgt
     2461 ccgggcagcg gtttccggtg aagtattacc tgaccgcaat gctatttatc gtcttcgaca
     2521 tcgaaatcgt gttcttgtat ccctgggcgg ttagctacga cgcgctggga acgttcgctc
     2581 tggtcgagat ggtggtgttc atgctcacgg tcttcgtggc ctatgcctac gtgtggcgcc
     2641 gcggcggcct gacgtgggat tgaggtaaga cgtgggcctg gaagaacagc tgcccggtgg
     2701 gattctgctg tcaactgtcg agaaggtcgc gggctacgtc cgcaagaact cgctgtggcc
     2761 ggcgaccttc gggttggcct gctgcgcgat cgagatgatg gcgaccgccg ggccgaggtt
     2821 cgacatcgcg cggttcggca tggaacggtt ctcggcgacg ccgcggcagg ccgacctgat
     2881 gatcgtggcc gggcgggtca gccagaagat ggcgcccgtg ctgcggcaga tctacgacca
     2941 gatggcagag ccgaagtggg tgctggccat gggggtgtgc gcgtcatcgg gcgggatgtt
     3001 caacaactac gcgatcgtgc agggcgtgga ccatctcgtt ccggtggaca tctaccttcc
     3061 cggctgcccg ccgcgcccgg agatgctgtt gcacgcaatc ctcaagctgc acgagaagat
     3121 tcaggaaatg cccctgggcg tcaatcgcga acacgccatc gcggaggccg agcgggcggc
     3181 actggcggcc aggccaacga tcgagatgca cggattgctg cgatgagctc gcccagccaa
     3241 gacccgcggg aagcagttac cgggcccgat gacgaggtga tcgacgttcg tcgcggcatg
     3301 ttcggtgtgt ccggcaccgg tgacacctcc ggatacggac ggctggtgcg cgaggtcgcg
     3361 ctgccgggca gcagttccag gccctacggc ggctacttcg acgacatcgc cgaccggctc
     3421 gccgaggcgc tacggcgcga tgatgtcgaa ttcgatgacg cggtagagaa agtcgtcgtc
     3481 gaccgcgacg aactgaccct gcacatctgc cgggacctgc tgccgcaggt tgcacagcgg
     3541 ctgcgcgacg aaccggacct gcgcttcgag ctgtgcctgg gcgtcagcgg ggtgcactac
     3601 ccgcacgaga ccggccggga gctgcacgcc gtctacccgc tgcaatcgat cacccacggc
     3661 cgtcgcctcc ggctggaagt ggctgcgccg gacggtgatc cgcatatccc gtcgctgttt
     3721 tcggtctacc cgaccaacga ctggcacgag cgcgagacct acgacttctt cggaatcatc
     3781 ttcgacggcc atccgtcgct caccaggatc gagatgcccg atgactggca ggggcatccg
     3841 caacgcaagg actacccact gggcggcatt ccggtcgagt acaagggcgc gcagataccc
     3901 ccacccgacg agcgcagggg ctacaactga tgagcgacac caccgcaacg gcgtacgggg
     3961 acgacgccgc tgagacggtc ctggtcgcgg gtggtcagga ctgggagcag atcatcgacg
     4021 cggcacgcaa ctcggacccc ggcgaacgca tcgtcgtcaa catgggaccc cagcaaccgt
     4081 ccacctacgg ggtgttgcgg ctgatcctcg agatcgaggg cgagaccgtc atttcggcca
     4141 gatgcggaa
//
LOCUS       DASSGQ010000230        12108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_106921,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000230 DASSGQ010000000
VERSION     DASSGQ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12108
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106921"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="VFI55_10025"
     CDS             complement(<1..149)
                     /locus_tag="VFI55_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HET7074401.1"
                     /translation="MADNTTTPAGEQGTDSAAESEPDYRFTMANERTFLAWQRTALGL
                     LAAAVA"
     gene            257..958
                     /locus_tag="VFI55_10030"
     CDS             257..958
                     /locus_tag="VFI55_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HET7074402.1"
                     /translation="MVKALLFDVQGTATDFHSTVCDEAQRISAGRHPGVDWAEVVRRW
                     RASYFAVLAAAQGRSGGWVSVHSVYRDALDSVLDESGITGFSDAEREELTLAWQRLKP
                     WPDVVPGLTRMKRAHTLATLSNADVSAVVNISKRAGLPWDAVFAAEMAGVFKPDPAIY
                     HLAATYLGLDPAEIMMVASHKYDIRAAGLLGFATAFVARPLEFGVGAEADVAYSDEFD
                     VNASDFLDLAEQLGC"
     gene            complement(987..1376)
                     /locus_tag="VFI55_10035"
     CDS             complement(987..1376)
                     /locus_tag="VFI55_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074403.1"
                     /translation="MTATAAPAPPGARIVKWIDLQVGDCVADLPPADLSRVTVTVVDC
                     ATAHLAEVYLRAPMAVDSAIANVANRDCAAGFASYTGHSVDGTPFSITFLIDSNQDRT
                     GANPTPSTVICLLQGANGQALTGSAHR"
     gene            complement(1489..2052)
                     /locus_tag="VFI55_10040"
     CDS             complement(1489..2052)
                     /locus_tag="VFI55_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET7074404.1"
                     /translation="MTIPAFPAPEVLAARQKLVLDHFHDEVRQAWDDVLSTFPHPRYE
                     LIPQLVVHDGDEAVRGYYAYTRTAFPDQDHEIIALRHSADAVIVEFWLMGTHRGYLGK
                     VPPTGSRFRVRMTAYFVFDDTETLVCERIYFDTLTMVKQLLGGLDMKKPANWLLAARS
                     LRGFLSMSSEKPDPALTSSANTPLSGS"
     gene            complement(2141..3439)
                     /locus_tag="VFI55_10045"
     CDS             complement(2141..3439)
                     /locus_tag="VFI55_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7074405.1"
                     /translation="MSITTGIATRVNGAPPPEIPLADIHLESLEFWGLDDDYRDGAFA
                     TLRREEPISLRPAIEMEGFIAGNGYWALTKHDDVHYASRHPDIFSSSPNITINDNAPE
                     IAEYFGSMIVLDDPRHQRLRSIVSRAFTPKVVARIEASVRERAHRLVASLIANHPDGE
                     ADLVSELAGPLPLQIICDMMGIPEEDHQRVFHWTNVILGFGDPDLTTDFEQFLQVSMD
                     IGAYASALADDRRVNHRDDLTTSLVEAEVDGDRLTSSEIASFFILLVVAGNETTRNAI
                     SHGVLALSRYPEQREKWWSDFDRLAPTAVEEIVRWASPVVYMRRTLTRDVELGGVRMV
                     AGDKAALYYNSANRDESKFANPWAFDVARDPNPHLGFGGGGAHFCLGANLARREIRVA
                     FDELRREIPDIVAIEEPARLLSQFIHGIKRLPVAWTPPRR"
     gene            complement(3507..3854)
                     /locus_tag="VFI55_10050"
     CDS             complement(3507..3854)
                     /locus_tag="VFI55_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmpS family transport accessory protein"
                     /protein_id="HET7074406.1"
                     /translation="MTSHLRGPAILFAIGIGLANMCGVANAAKDFPTVRYEVSGPATA
                     QFIYYQTGAGQLRAVNATLPWSAEFTGYGGGVYVLSAQGPGPISCKILLDGNVVSNAT
                     ATTGTPARTVCTH"
     gene            complement(3902..4234)
                     /locus_tag="VFI55_10055"
     CDS             complement(3902..4234)
                     /locus_tag="VFI55_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HET7074407.1"
                     /translation="MTVIQEHTPLATLINVFTVAPERAPQLAELLRVATEEKMRFVPG
                     FISANIHVSTDGTRVVNYAQWQSADAYQAMFDDPSAREHMGRCAAIATSFEPHLYTVE
                     SVHHAAHS"
     gene            4302..4745
                     /locus_tag="VFI55_10060"
     CDS             4302..4745
                     /locus_tag="VFI55_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7074408.1"
                     /translation="MSSSQTEHGPGYLVKRVQQELRRRCDAALKPTGLSMAQYALLRA
                     LHDHPDASASELARLCFVTRQSLRDVLNGLRTAGLVAASDAQGRGRAQALQLTSLGLR
                     HLNNSHAAVEGVEAEMLRGIPSAGRKELAAMLIRCAENLEALPDR"
     gene            4816..5901
                     /locus_tag="VFI55_10065"
     CDS             4816..5901
                     /locus_tag="VFI55_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HET7074409.1"
                     /translation="MSERGPKRVVVWGTGFVGRMVINEILKHPRFELVGVGVSNPDKV
                     GRDVGEICGQPEPVGITATDDVEALIALRPDALVHYGPTAAHADANIALITRFLRAGI
                     DVCSTSMTPWVWPTMYLNPPNWIEPITKACELGGSSCFTTGIDPGFANDLFPMTLMGV
                     CSEVRKVRASELLDYTNYEGDYETEMGIGREPEFRPILENPTVLIFAWGATVPMIAHA
                     AGIMLDEITTTWDKWVTPTERTTAKGVIKPGHVAAVRFTINGIYRGETRIQLEHVNRI
                     GHDAAPDWPSGHDNDVYRVDIEGTPSIFQETAFRFTDGSGRDAAAAGCLATGMRALNA
                     VQAVNDLPPGWVTPLDLPLIAGEGSIR"
     gene            6075..7919
                     /locus_tag="VFI55_10070"
     CDS             6075..7919
                     /locus_tag="VFI55_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET7074410.1"
                     /translation="MAEGAKPALGLSIGATNLAAVTADLAIIRKPVLTLFRRRAPEVG
                     IPSENPKLDEPGLVIADFVDRVGDPVGIVAADGSVHRSEALVTDALRALAYAATRPLP
                     ENIAVTYPAHWSPKAVDVLGASLTRASERSNRGRPPALMSDAAAALFAARANPGIPAR
                     GTVAVCDFGGSGTSITLMDAAGDYDPVAPTVRHTDFSGDMIDQALLTAVMADMPNTGS
                     FDPSGTSAIGSLNRLRTGCRIAKEQLSSSTVTTLTDDLPGMPGGIRLTRNELDDAIRK
                     PLSNFVAVLDEALQRNGIRDLVAVITVGGGASIPAVTTALSGHLRIPVVTNPRPQLTA
                     AIGGALRASAAPGENIATALTPAAPEAASALIPVAWAAKVQSEPAQAQSGTPEGPVSN
                     MMPALAWSEAEDESRALPAGAGVYPEGGRSGYTSARPAVKFQQPAPPRSESKKSVMPW
                     YRLPVVLIIGTAVAILLVGTAVAIGLNSGDSTAPAPGVSTTPASSVPPAGSASPQPPN
                     TGQSAPPAQQAPPPSSADTNPPATTDPPTTGNPPAAPEGPVNQAPDPPAPEAPPPPPA
                     PAPAPPPIPAIPPIPAIPRIPGIGAPIPYLGPGLGARLGIGPIIQQGN"
     gene            complement(7937..8308)
                     /locus_tag="VFI55_10075"
     CDS             complement(7937..8308)
                     /locus_tag="VFI55_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074411.1"
                     /translation="MNVQEDKAARQDHDQRPTEKPQPDEDNKRRAADMMTAYEDRPTL
                     VLPGSGGMVSGTAVNDWLDENGNPKYGAPGSEDKADSTDERVAAHGQSGGLDKEQIEK
                     DKALNDELRKASAAENKGEKR"
     gene            complement(8383..9441)
                     /locus_tag="VFI55_10080"
     CDS             complement(8383..9441)
                     /locus_tag="VFI55_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET7074412.1"
                     /translation="MVRPQPRDSATYTQSSRLQAAGLARAIAVFERAAEQVPLPTNPQ
                     PIAIADYGTANGHNSLKPLSAAIAVLRRRTRHDQAILVAHTDVPDNDFTALFRTLSDD
                     PDSYLHMDAASFSSAIGRSFYGQILPSKTINLGWTSWATQWVSRVPCELRDHVHVAYS
                     KDNAARAAYAHQAALDWNDFVAFRGRELAPGGRLVALTPAVDEDGTSGFRALLDAIVA
                     TLDDRAREGLLTMDELRRMAIPAFARTEADFRAPFSPSGRFEGLTIEHLETFNAEDRF
                     WARFQTDDDAEAFGAQWAAFARAVLFRYLTGGLDCAPNDPRAIEFVEQLETAVAERLS
                     KAPEPMRIPLASVVLVKR"
     gene            complement(9530..10483)
                     /locus_tag="VFI55_10085"
     CDS             complement(9530..10483)
                     /locus_tag="VFI55_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7074413.1"
                     /translation="MAKDLVATVPDLSGKLAVVTGANSGLGFGLTRRLAAAGADVVMA
                     IRNRAKGEAAIAEIRTTVPDAKLTIKALDLSSLAAVAALGEQLNAEGRPIDILINNAG
                     VMTPPERDTTADGFELQFGSNHLGHFALTGHLLPLLRAANGARVVSLSSLAARFGRIQ
                     FDDPQFEKSYSSMASYGQSKLAVLMFALELDRRNRAGGWGIVSNAAHPGLTKTNLQVS
                     GPSHGRDKPALMERFYKASWRFTPFLWQEIDDGILPALYAAAAPHAEGGAFYGPRGPF
                     ESAGGGVAPAKVPARARNQDDCRRLWELSTQLTAVSYPKPN"
     gene            10585..11376
                     /locus_tag="VFI55_10090"
     CDS             10585..11376
                     /locus_tag="VFI55_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HET7074414.1"
                     /translation="MSSNRLAKTSLSAAVVMMVVSVAGFIIAIVLNTFFLDKYNAYGE
                     VPIPGSGSLYLPAGEVNLSLHTVLLGSPNGGGLPVPPLGVTISPPDGVAQPVVTENYG
                     STTTVNNDAHVRVWVAKIPVSGTYNITTDGQVNGYIDPRLAFGHQSSYGFLVWLFVAM
                     FVVGLVGSIISGRWMARARRKAVTSSPYFESSMPFSESSMPFSPVTPTVLPPATPEAP
                     VTTASEPSDEGVRLERLKTLAALRDSGALTQQEFETEKRRILQGY"
     gene            complement(11463..>12108)
                     /locus_tag="VFI55_10095"
     CDS             complement(11463..>12108)
                     /locus_tag="VFI55_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7074415.1"
                     /translation="VDPQADPAVAGLLGCGLMAGLGAAINTGAVDRNDTVAVIGCGGV
                     GDAAIAGAALVGARRIIAVDTDNTKLDLARKFGATHTVNAREFDVVKTIQDLTDGFGV
                     NVAIDAVGRPETWKQAFYARDLAGTVVLVGVPTPDMRLDMPLLDFFSHGGSLKSSWYG
                     DCLPERDFPTLISLYLQGRLPLEKFVSERIGLGDVEEAFRKMHDGNVLRSVVML"
BASE COUNT         2020 a         3930 c         4011 g         2147 t
ORIGIN      
        1 gcgactgccg ccgcgagcaa acccagtgcg gtgcgctgcc aggccaggaa ggtccgctcg
       61 ttggccatgg tgaaccggta gtcgggctcg gactctgctg cgctgtcggt gccctgctcg
      121 ccggcgggcg ttgtggtgtt atcggccacg gggtacctcg cagggcgtat gagggcaagg
      181 gttttgacgt cgcggatttt aaccaaccat acggctcgcc acgtggcgtt tcaccgccgc
      241 cctgcatgat gagaatgtgg tcaaggcgtt gttgttcgac gtgcagggca cggcgacgga
      301 ttttcattcg accgtgtgcg acgaggcgca gcggatcagc gctgggcggc acccgggcgt
      361 cgattgggcc gaggtggtgc ggcgttggcg cgcaagctac ttcgcggtgt tggcagccgc
      421 gcagggtcgc agcggtgggt gggtttccgt gcactcggtg tatcgcgacg cactggacag
      481 cgtcctggac gagtccggca tcactgggtt ttccgacgcc gaacgtgagg agctcacgct
      541 ggcctggcag cggctgaagc cgtggccgga cgtggtgccc gggctgactc gaatgaagcg
      601 cgcgcacact ctggccaccc tgtccaacgc cgacgtgtcc gcagtggtca acatctccaa
      661 gcgagcgggg ctgccctggg acgcggtctt tgccgcggag atggccggtg tcttcaagcc
      721 cgacccggcg atctatcacc tggcggcgac ctaccttggc ctggatcccg ctgagatcat
      781 gatggtcgcc agtcacaagt acgacattcg tgccgcgggt ctgctcggct tcgcgaccgc
      841 cttcgttgcg cgcccgctgg aattcggggt cggcgccgaa gccgacgtgg cttattcaga
      901 cgagttcgac gtcaacgcgt ccgacttcct ggaccttgcc gaacaactcg gctgctaacg
      961 cgcgatgctg ccgcgctgga cttgtgtcag cgatgcgccg atccggtcag ggcttgaccg
     1021 ttggcgcctt gcagcaggca gattacggtg ctcggagtgg ggttcgcccc ggttcggtcc
     1081 tgattcgagt cgatcagaaa tgtgatcgaa aacggggtgc cgtcgacgga atggccggtg
     1141 taagaggcga atccggccgc gcaatcccga ttggcgacgt tggcgatggc gctgtcgacc
     1201 gccatcgggg cgcgtagata cacttcggcc aaatgcgctg tggcacagtc gacgacagtg
     1261 accgtcaccc ggctcagatc agcaggcggg aggtcggcca cgcaatcgcc gacctgcagg
     1321 tcgatccact tcacgatccg cgcgcccggc ggtgcgggag ccgcggtggc ggtaatcgtg
     1381 gcaccggccg tcagcagtgt cgctgcggtc aggccgcata acgcgtgaag gcttcggacc
     1441 cgcaacgcag cctcctcgat ccatcgctta gcggtggagt ctaggcattc agctccccga
     1501 cagaggcgta ttcgcagaag aggtcagcgc tgggtccggc ttttccgacg acatcgacag
     1561 aaatccccgc aggctgcgcg ctgccagcaa ccagttcgcc ggcttcttca tatccaggcc
     1621 gcccagcagc tgtttgacca tggtcagcgt gtcgaagtaa atgcgctcgc agacaagcgt
     1681 ttccgtgtcg tcgaacacga agtatgcggt catgcggacg cgaaagcggc tgccggtcgg
     1741 cggaaccttg cccaggtagc cgcgatgggt gcccatcagc cagaactcga cgatcaccgc
     1801 atcggcgctg tgccgcagcg cgatgatctc gtggtcctga tcgggaaacg ccgtccgcgt
     1861 gtaggcgtaa taaccgcgca ccgcctcgtc gccgtcgtgg acgaccaact gcgggatcag
     1921 ctcgtagcgc ggatggggga aggtcgataa cacgtcgtcc caggcctgtc ggacctcgtc
     1981 atgaaagtgg tcgagcacga gtttctggcg ggcggccagc acctctggag cgggaaacgc
     2041 gggaatggtc acggtggtca gggtagaccc ggggatgttc aggaatttga tagcgcattc
     2101 gctatcaaat tcctgaacag ggtgttcctt cggacagtcg ctaacgccgc gggggagtcc
     2161 aggccaccgg caacctcttg atgccgtgga tgaattgcga cagcagtcgt gccggttcct
     2221 cgatcgcgac gatgtcgggg atctcgcggc gcaattcatc gaaggcgact ctgatctcac
     2281 ggcgagccag gttggcgccc aggcagaaat gggcgcctcc gccgccgaag ccgagatgcg
     2341 ggttcggatc gcgcgcgacg tcgaaagccc acggattggc gaactttgac tcgtcacggt
     2401 tggccgagtt gtagtagagc gcggccttgt ccccggcgac catcctgacg ccacccagtt
     2461 cgacgtctcg ggtcagcgtg cgtcgcatgt agaccaccgg cgacgcccag cgcacgatct
     2521 cctcgaccgc ggtgggcgcc aggcgatcga agtccgacca ccacttctcc cgttgctcgg
     2581 ggtaacggga gagagccagc actccgtggc tgatcgcgtt gcgcgtggtc tcgttgccgg
     2641 ccaccaccaa caggatgaag aaggacgcga tttccgacga ggtcagccgg tcgccgtcga
     2701 cctcggcttc caccaggctc gtcgtcagat cgtcacggtg attgacgcgg cggtcgtccg
     2761 ccagcgcgct ggcgtaggcg ccgatgtcca tcgaaacctg aagaaactgt tcgaaatccg
     2821 ttgtcagatc ggggtcgccg aacccgagaa tcacgttggt ccagtgaaat acccgctgat
     2881 ggtcctcttc gggaatcccc atcatgtcgc agatgatctg tagcggcagt ggcccggcga
     2941 gctcgctgac cagatcggcc tcgccgtcgg gatgattggc gatcagcgag gcgaccagtc
     3001 ggtgggcccg ctcgcgcacc gacgcctcga tgcgggccac tactttcggg gtgaacgccc
     3061 ggctgacgat cgagcgcagc cgctggtgtc gcgggtcgtc gagcacgatc atcgacccga
     3121 agtactcggc tatctccggc gcattgtcgt tgatggtgat gttcgggctg gagctgaaga
     3181 tgtccggatg gcggctggcg tagtgaacgt cgtcgtgctt ggtcagcgcc caatacccgt
     3241 tgcccgcgat gaacccttcc atctcgatcg cgggccgtaa cgagatgggc tcctcgcggc
     3301 gcagggtggc gaaagcgccg tcacggtagt cgtcgtcgag tccccagaac tcgagcgact
     3361 cgagatggat gtcggccagc ggaatctcgg gcggtggcgc tccgttcact cgagtcgcga
     3421 tacccgtcgt gatgctcacc tgtgccctcc ttcgtcttga gaacagattc gaagaggaga
     3481 cacggggtag cccttgtcag tgcgcgtcag tgcgtacaga cggtcctggc tggtgtcccc
     3541 gtcgtcgcgg tcgcgttgct caccacattg ccgtccagca gaatcttgca gctgatcggg
     3601 cccggcccct gcgcgctcag gacatacacg ccgccgccgt aaccggtgaa ctcggccgac
     3661 cacggcagtg tcgcgttgac cgcacgcagc tgaccggcac cggtttggta gtagatgaat
     3721 tgggcggtgg cgggaccgct cacctcatag cgaactgtgg gaaagtcctt tgccgcgttg
     3781 gccacgccgc acatgttcgc gagtccgatg ccgatggcga agagtatcgc gggtcccctg
     3841 agatgactgg tcatgaatgt catcgtgtca tcacgtcaga cggtacggaa gtgctttcta
     3901 cctagctgtg tgccgcgtgg tgcaccgatt ccacggtgta gaggtggggt tcgaagctgg
     3961 tggcaatagc ggcgcagcgg cccatgtgtt cgcgcgcgct cggatcgtca aacatcgctt
     4021 ggtacgcgtc ggcgctttgc cattgtgcgt agttgaccac gcgcgtgccg tcggtgctga
     4081 cgtggatatt ggcggagatg aagccgggca cgaaccgcat cttctcttcg gtggcgactc
     4141 gcagcagctc ggccaattgc ggcgcccgct ctggcgccac ggtgaagaca ttgatcaggg
     4201 tcgcgagcgg agtgtgctct tgaatcacgg tcatcgtcga tcgcctccag aattgtcagg
     4261 aaccttacac ctagaccata acacgatgta aggttcctgt catgtcctct tcgcagaccg
     4321 aacatggccc gggttatctg gtgaagcggg ttcagcagga actgagacgt cgctgcgacg
     4381 ctgcgctcaa acccaccggg ctgtcgatgg cccagtacgc gctgttgcgt gccctgcatg
     4441 atcatcccga cgcgtcggcc tccgaactcg cccggctgtg cttcgtcacg cgtcaatcgc
     4501 tgcgcgatgt tttgaatggg ttgcgtacgg cgggtctggt tgccgcgtcg gacgcacagg
     4561 gaaggggtcg cgcccaggcg ctgcaactga cctcgctcgg tctgcggcat ctcaacaaca
     4621 gccacgccgc agtcgaaggc gttgaagccg agatgttgcg tggaattccg tccgcaggcc
     4681 gaaaggaact cgcggccatg ctcattcgct gcgccgaaaa cctcgaggcc ctgccggacc
     4741 gctgaccggt accgatgcct ccagtgcccg cgcctagggt gaaaccggga aggggctgat
     4801 gatcggaggg tgggtatgtc ggaacgcggt ccaaagcgag tagtggtctg gggcactggt
     4861 ttcgtcggca ggatggtgat caacgagatc ctcaaacatc cgcggttcga gttggtgggc
     4921 gtcggcgtca gcaaccccga caaggtcggc cgcgacgtgg gcgaaatctg cgggcagccc
     4981 gagccggtgg gcatcaccgc aaccgacgac gtcgaggccc tgatcgcgct tcggcccgac
     5041 gcgttggtcc attacggccc gacggcggca cacgcagatg ccaacatcgc actgatcact
     5101 cggttcctgc gggccggcat cgacgtctgc tcgacctcga tgacaccgtg ggtctggccg
     5161 accatgtatc tcaacccgcc caactggata gagccgatca ccaaggcatg cgagctgggc
     5221 ggatcgtcct gtttcaccac cggcatcgat ccgggattcg cgaacgacct ttttcccatg
     5281 acgctgatgg gcgtctgctc ggaggtgcgc aaggtgcgcg cctccgagtt gctggattac
     5341 accaactacg aaggcgacta cgagactgag atgggcatcg gccgtgagcc ggaattccgc
     5401 ccgatactgg aaaaccccac ggtgctcatc ttcgcgtggg gcgccacggt cccgatgatc
     5461 gcccacgccg ccggcatcat gctcgacgag atcaccacca cctgggacaa gtgggtgaca
     5521 ccgacggagc gcacgaccgc gaagggcgtc atcaagcccg ggcacgtcgc cgccgtccgg
     5581 ttcaccatca acgggatcta ccggggcgaa acccgcatcc agctcgaaca cgtcaaccgc
     5641 atcgggcacg acgccgcccc cgactggccg tcgggccacg acaacgacgt ctaccgagtc
     5701 gacatcgaag gcaccccgag catcttccag gagaccgcgt ttcggttcac cgacggatcg
     5761 ggccgggatg cggccgcggc cgggtgcctg gcaaccggga tgcgggcact caacgccgtc
     5821 caggcggtca acgatttgcc gcccggctgg gtcacgccgc tggaccttcc gctgatcgcc
     5881 ggcgagggct cgattcgctg attgggccgc atagcagcgg ctatgtcgtt atacagctca
     5941 cgcatagcat gcgcagagat tggctatagg tatcagcggc acaattgcaa ctgggcggat
     6001 gccgggcgtg gcgatcgcaa acgtggcggc gggtcgtcac gaagcggtgg ccgttgaacg
     6061 tagggattgg acgaatggcc gagggagcta agcccgcgtt gggcttgtcg attggtgcta
     6121 ccaacctggc tgccgttacc gcggatctcg cgatcatccg caagccggtc ctgacgctgt
     6181 tccgccggcg tgcccccgag gtcggcatcc cgtcggaaaa ccccaagctc gacgaacccg
     6241 gcctggtgat cgccgacttc gtcgacaggg tcggagaccc ggtcggaatc gtggcggccg
     6301 acggctcggt acaccgcagc gaggccttgg tcaccgacgc cttgcgtgcg ctggcctacg
     6361 ccgccacccg cccgctgccc gagaacatcg cggtgacgta tcccgcccac tggtctccga
     6421 aggccgtcga cgtcctgggc gcctcgctga cccgggcgtc tgaacggtcc aaccgcgggc
     6481 ggccgccggc gctgatgtcc gatgccgctg cggcactgtt cgccgcgcgg gccaatccgg
     6541 gcatacccgc gcgcgggacc gtggccgtgt gcgacttcgg cggcagcggc accagcatca
     6601 cgttgatgga cgccgccggc gactacgacc ccgtggcccc caccgtgcga cacaccgact
     6661 tctccggcga catgatcgat caggcgctgt tgaccgctgt catggccgac atgccgaaca
     6721 cgggttcgtt cgacccgtcg ggcacctcgg cgataggctc gctgaatcgg ctgcgaacag
     6781 ggtgccgcat cgccaaggag cagctctcgt cgagcacggt gaccactctc accgacgatc
     6841 tgcctggcat gcccggcggc attcggctca ccaggaacga actggacgac gcgatccgca
     6901 aaccgctgag caacttcgtg gcagtgttgg acgaggcctt gcagcggaac ggaattcgcg
     6961 atctggtcgc ggtcatcacg gtgggcggtg gggccagcat cccggcggtt accacggcgc
     7021 tgtccgggca cctccgcatc ccggtcgtga cgaacccgcg tccgcaactg acggcagcca
     7081 tcggcggcgc gctgcgggcg tcggcggctc ccggcgagaa catcgcgacc gcgctgaccc
     7141 cggccgcccc cgaagcggcg tcggcgctga tcccggtggc gtgggcggcg aaggtccaat
     7201 cggagccggc gcaggctcag tcggggacgc ccgaaggacc ggtatcgaac atgatgcccg
     7261 cgctggcctg gtcggaggcc gaagacgagt cacgcgccct acccgcgggg gcgggcgtgt
     7321 atccggaagg cggccgttcc ggctacacgt cagcccgtcc cgctgtgaag ttccagcaac
     7381 cggcaccacc gcgttcggag tcgaagaagt cggtcatgcc ctggtatcgg cttccggtgg
     7441 tgttgatcat cggtacggcg gtggcgatct tgctggtggg cacggcagtc gcgatcggat
     7501 tgaactccgg cgacagcacc gcaccggccc cgggggtgag tacgactccg gcctcctctg
     7561 tgccgccggc aggtagcgca agtccccagc cgccgaacac cgggcagtcc gcaccaccgg
     7621 cgcaacaggc gcccccgccg tccagcgccg ataccaaccc gccggccaca accgatccgc
     7681 cgacgaccgg caacccgccg gcggcgccag aggggccggt gaatcaggcc ccggacccgc
     7741 cggccccgga ggcaccgcca ccaccaccgg ccccggctcc ggctccgccg ccgattccgg
     7801 cgattccgcc cattccggcg attccacgaa ttcccgggat cggcgcgccc atcccgtatt
     7861 tgggccccgg cttgggcgcc cggctaggaa taggaccgat catccagcaa ggcaattagc
     7921 cgaattagcc gtcggctcag cgcttttcgc ccttgttctc cgccgctgag gccttcctca
     7981 gttcgtcgtt cagggctttg tccttctcga tttgttcctt gtcgagtccg ccgctctggc
     8041 cgtgggcggc cacgcgttca tcggtcgaat cggctttgtc ttccgagccg ggggctccgt
     8101 atttaggatt gccgttctcg tccagccaat cattgaccgc ggtgcccgac accattccgc
     8161 cggaacccgg aagcaccaac gtgggccggt cctcgtaggc cgtcatcatg tcggcggcgc
     8221 gtcgcttgtt gtcctcgtcc ggctgcggct tttcggttgg ccgctgatca tgatcctgac
     8281 gagccgcttt gtcttcctgc acgttcatcc gtctacccct tgcagtcgca ggtgcgacca
     8341 tcgtccgggg ttacccgctg cagtgttgtg tcgaaaccct tgtcagcgct tgaccagcac
     8401 gaccgaggcc agcggaatcc gcatcggctc gggtgccttt gacagccgtt ccgcgacggc
     8461 ggtctccaat tgctcgacga actcgatcgc tcgggggtcg ttgggcgcgc agtccagccc
     8521 gcccgtcaga taacggaaaa gtacggcccg cgcaaacgcc gcccactgcg cgccgaaagc
     8581 ctcggcgtcg tcgtcggtct ggaaccgagc ccagaatcgg tcctcggcgt tgaaggtctc
     8641 gagatgctcg atcgtcaagc cttcgaaacg gccgctgggc gaaaacggag cgcgaaagtc
     8701 cgcctcggtg cgggcgaatg ccggaatggc catccgccgc agctcgtcca tggtcagcag
     8761 gccctcgcgt gcccgatcgt caagtgtcgc aacaatcgcg tcgagcaacg ccctgaaccc
     8821 ggatgtgccg tcctcgtcga cggcgggcgt caacgccacc agccgccctc cgggggccaa
     8881 ttcgcggccg cggaacgcca cgaagtcgtt ccagtcgagg gcggcctggt gggcgtaggc
     8941 ggcgcgggcc gcgttgtctt tgctgtaggc gacgtgcacg tgatcgcgga gttcgcaggg
     9001 gacccggctc acccattgcg tcgcccagga cgtccagccg aggttgatcg tcttggacgg
     9061 caggatctgg ccgtagaagg accggccgat cgccgaggaa aaactagctg cgtccatgtg
     9121 caggtagctg tccgggtcgt cggacaaggt ccgaaacagc gccgtgaaat cgttgtccgg
     9181 tacgtcggtg tgcgccacga ggattgcttg gtcgtggcgg gtgcgacgac gcagcaccgc
     9241 gatggccgcc gacagcggct tcagcgaatt gtgcccgttg gcggtgccgt agtcggcgat
     9301 ggcgatcggc tgtggattcg tcggcagcgg cacttgttcc gcggcccgct cgaacaccgc
     9361 gatggctcgc gccagcccgg cggcctgcaa ccgcgacgac tgggtgtatg tggcgctgtc
     9421 cctaggctgt ggtcggacca caatcgtcga ctggggagtc gattgggggg tcggttcggg
     9481 cgtccgacgc tcaggccgca tggacacacg ataatcggtg cggttgttgt cagttcggct
     9541 tcgggtagct cactgcggtg agctgcgtgg aaagctccca cagtcgtcgg cagtcgtcct
     9601 ggttgcgggc acgcgcgggc accttggccg gcgccacgcc accgccggcg gactcgaagg
     9661 gcccgcgtgg tccgtagaag gcgccgccct cggcgtgcgg cgcggcggcc gcgtacagcg
     9721 ccggcagaat cccgtcgtcg atctcctgcc acaggaatgg cgtgaaacgc cacgacgctt
     9781 tgtaaaaccg ttccatcagc gcgggcttgt cgcggccgtg cgacggtccg ctgacctgca
     9841 ggttggtctt ggtcaatccg ggatgcgcgg cgttggacac gatgccccaa ccgcccgcgc
     9901 ggttgcggcg gtccagctct aacgcgaaca tcaaaacggc cagcttcgac tggccatagg
     9961 acgccatcga cgagtaggac ttctcgaact gcggatcgtc gaactggata cggccgaagc
    10021 gggccgccag gctgctcagc gagaccaccc gcgcgccgtt ggcggcgcgc aacaacggca
    10081 gcaggtgtcc ggtcagcgcg aaatggccga gatggttact gccgaactgc aattcgaagc
    10141 cgtcggcggt ggtgtcccgt tcgggcggcg tcatcacacc ggcgttgttg atcagaatgt
    10201 cgatcgggcg accctcggcg ttgagctgtt cgcccagcgc ggcgacggcc gccagtgacg
    10261 acaggtcgag ggccttgatc gtcaacttcg cgtcgggaac cgtggtgcgg atttctgcga
    10321 tcgccgcttc acccttggcg cgattgcgga tcgccatgac gacgtcggcg ccggcggccg
    10381 ccagccgccg ggtgagcccg aaccccagac cactgttggc gcccgtgacg actgccagct
    10441 tgcccgacag atcgggcacg gtagcgacga gatccttggc catgcgtatc actcctttgt
    10501 tcggtatgcg ccccgtccca gtctgctctt tccggcgccg tcggcgaact ggcatcgtct
    10561 ggcccgcgat gacacactgc ctggatgagc agcaacaggc tcgcgaagac ttcgctgtcg
    10621 gccgccgtcg tgatgatggt cgtctcggta gccggcttca tcatcgcgat agttctcaac
    10681 accttcttct tggacaaata caacgcgtac ggcgaggtgc cgatcccggg gtcaggcagc
    10741 ctgtacctgc ccgcgggtga agtcaacctg agcctgcaca ccgttctgct cggcagtccc
    10801 aacggcggcg gactgccggt gccgccgctc ggcgtcacga tctcgcctcc cgacggcgtg
    10861 gcccaaccgg tggtcacgga aaactacggc agcacaacga cggtcaacaa cgacgcgcac
    10921 gtgcgagtgt gggtggcgaa gattcccgtg agtggcacct acaacatcac gactgatggc
    10981 caagtcaacg gatacatcga cccgcggctg gccttcggcc accagagttc ctacggcttc
    11041 ctggtgtggt tgttcgtcgc gatgttcgtt gtgggacttg tcggttcgat catctcggga
    11101 cgctggatgg ctcgcgcccg gcgcaaggcg gtaacgagta gcccctactt cgaatcttcg
    11161 atgccgttct cggaatcttc gatgcccttc tcgccggtca cgccgacggt cttgccaccc
    11221 gccacgcccg aagcgccggt caccacggcc tccgagccca gcgacgaggg ggtccgcctc
    11281 gagcgactca aaacgctggc ggcgctgcgg gattccgggg ccttgacgca acaggagttc
    11341 gagaccgaga agcgccggat cctccagggg tactgaattt cgccgccaga gcacgtcgac
    11401 ttcccaactg ccaccgtcga gttcgaaggg cccgtgggtg accacttggt cgatcgcggc
    11461 cgttacagca tgactaccga acgcagcaca ttgccgtcgt gcattttgcg gaacgcctcc
    11521 tcgacgtcgc cgagcccgat gcgttcggac acgaacttct ccagcggaag ccggccctgc
    11581 aggtagaggc tgatcagagt ggggaagtcg cgttcgggca ggcaatcgcc gtaccacgac
    11641 gacttcagcg agccgccgtg gctgaagaag tccagcagcg gcatgtccag gcgcatgtcg
    11701 ggcgttggaa ccccaaccag cacaacggtt ccggcgaggt cgcgggcgta gaaggcctgc
    11761 ttccaggttt cgggccggcc caccgcgtcg atcgccacgt tgacgccgaa gccatcggtg
    11821 aggtcctgga tggtcttgac gacatcgaat tcgcgggcgt tgacggtgtg ggtggcgccg
    11881 aacttgcggg ccaagtccag cttggtgttg tcggtgtcga cggcgatgat tcgtcgggcg
    11941 ccgaccagcg cggcccccgc gatcgccgcg tcgcccacgc ctccgcagcc gatcaccgcg
    12001 acggtgtcgt tgcggtcgac cgcgccggtg ttgatcgccg cccccagccc ggccatcaac
    12061 ccgcaaccca gcaggcccgc gacggccggg tcggcctgag gatcgacc
//
LOCUS       DASSGQ010000231        10643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1383480,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000231 DASSGQ010000000
VERSION     DASSGQ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10643
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383480"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="VFI55_10100"
     CDS             <1..177
                     /locus_tag="VFI55_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7074416.1"
                     /translation="SRSGSTAARLFYAYADTLLALGRNDEALHWFLRAAAADVEGVTD
                     AEDRAAELGPADKE"
     gene            174..1193
                     /locus_tag="VFI55_10105"
     CDS             174..1193
                     /locus_tag="VFI55_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HET7074417.1"
                     /translation="MKTLAQEYDCLLIDLDGTVFRGAQPTTGAVASLEEVHSRKLYVT
                     NNASRSADEVASHLRELGFTVSGADIVTSAQSAARLLADELPAQSRVLIVGTEALANE
                     IAAVGLRPVRRYDDHPVAVVQGLSTTIGWPDLAEAALAIRAGALWVAANVDPTLPTER
                     GLLPGNGSLVAALRTATGAEPRVAGKPAPTLFQEAAARGDFRASLVIGDRLDTDIEGA
                     NAAKLPSLMVLTGVNTAWDAVYAESARRPTYIGHDLRSLHQDGRLLAVGPQPGWHVDV
                     GGQAVAVSANGADDGDGLSIVRAVAAAVWGAPGADAQGRPVRIEAADDRAREALRRWA
                     LVHSD"
     gene            1217..1402
                     /locus_tag="VFI55_10110"
     CDS             1217..1402
                     /locus_tag="VFI55_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074418.1"
                     /translation="MNIDPDQLRAEIDSLIAQLPEVGDPENPESEPSLARLEEIARRL
                     SEAHDVLLQALESAEKG"
     gene            1411..2220
                     /locus_tag="VFI55_10115"
     CDS             1411..2220
                     /locus_tag="VFI55_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET7074419.1"
                     /translation="MARRARVDAELVRRGLARSRQQAAELIGAGKVTIDGLPARKPGT
                     AVALTAALTVAADGERSWVSRGAHKLIGALDAFGISVAGRRCLDAGASTGGFTEVLLD
                     RGATEVAAADVGYGQLAWSLRSDARVVVVERTNARDLSPEAIGGPVDVVVADLSFISL
                     ATVLPALIGCALPGADIVPMVKPQFEVGKGQVGAGGVVHDPGLRADAVLAVARRAGEL
                     GWHTVDVTASPLPGPSGNVEYFLWLRALTERPLSDEGLTNAVHRAVREGPQ"
     gene            2217..3152
                     /locus_tag="VFI55_10120"
     CDS             2217..3152
                     /locus_tag="VFI55_10120"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HET7074420.1"
                     /translation="MTPKTTAPRTILLVVHTGRDEATETARRVEKVLGDNGIALRVLS
                     AEAVDRGSLHLAPDDMRALGVDIQVVDADRHAAEGCELVLVLGGDGTFLRAAELARNA
                     GIPVLGVNLGRIGFLAEAEAEAIDLVLENVITRGYRVEERLTLDIMVRDGGRVIERGW
                     ALNEASLEKGPRLGVLGVVVEIDGRPVSTFGCDGVLVSTPTGSTAYAFSAGGPVLWPD
                     LEAILVVPNNAHALFGRPMVTSPDATIAIEIEASGNDALVFCDGRREMLLPAGGRLEV
                     TRCGTPVKWARLDSAPFTDRLVRKFRLPVTGWRGK"
     gene            3176..4951
                     /gene="recN"
                     /locus_tag="VFI55_10125"
     CDS             3176..4951
                     /gene="recN"
                     /locus_tag="VFI55_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET7074421.1"
                     /translation="MLTEIRIESLGAISVATAEFDRGFTVLTGETGTGKTMVVTGLHL
                     LGGARADATRVRSGADRATVEGRFTTTDLDDAAAAQLDAMLDASGAERDEDGSVIALR
                     SVSRDGPSRAYLGGRSVPAKSLSGFTAELLTLHGQNDQLRLMRPEEQRGALDRFAGAG
                     PAYERYRKLRDAWLSARRDLIDRRNRSRELAQEADRLRFALNEIDTAEPQPGEDDALV
                     ADILRLSELDTLREAAAIARASLSTDDADVSGLSATDSLGKARAALESTDDVTLQALG
                     RQIGEALTVVVDAARELGGFLDDLPVDASALESKLARQAQLRALTRKYAADIDGVMRW
                     AQEAREKLAQLDVSPEGLAGLQSRVDELERELSEAAVDLSKIRRKAAKRLGKEVTAEL
                     SGLAMADAEFTIDVSTDIATGQDDSAVLTLPSGELARAGGDGIDQVEFGFAAHRGMTV
                     LPLAKSASGGELSRVMLALEVVLATSRKQAAGTTMVFDEVDAGVGGWAAVQIGRRLAR
                     LARTHQVIVVTHLPQVAAYADVHLVVHSGGQGAGRSSGASGVRRVAGDERVAELARML
                     AGLGESDSGRAHARELLDAAQKDEI"
     gene            5129..6310
                     /gene="steA"
                     /locus_tag="VFI55_10130"
     CDS             5129..6310
                     /gene="steA"
                     /locus_tag="VFI55_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HET7074422.1"
                     /translation="MKMSGLLSRNSNRPGLTGTARVDRNIDRLLRRVCPGDIVVLDVL
                     DLDRITADALVDADIAAVVNASPSVSGRYPNLGPEVLVNNGVTLIDETGPDIFKKVKD
                     GSKIRLHEGGVYSGDRRLVRGTERTDHDIADLMREAKSGLAAHLEAFAGNTIEFIKSE
                     SPLLIDGIGIPDIDVDLRRRHVVIVADEPSAADDLRSLKPFIKEYQPVLIGVGTGADV
                     LRKGGYRPQIIVGDPDEISTEALKSGAHVVLPADADGHAPGLERIQDLGVGAMTFPAA
                     GSAVDLALLLADHHGASLLVTAGHTANIETFFDRTRTQSNPSTFLTRLRVGEKVVDAK
                     AVATLYRNHISAGAIALLALTMLIAVIVALWVSRTDGVVLHWIVDYWNHFSLMVQRWV
                     T"
     gene            6335..7279
                     /locus_tag="VFI55_10135"
     CDS             6335..7279
                     /locus_tag="VFI55_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HET7074423.1"
                     /translation="MISLRQHALSLAAVFLALAVGVVLGSGFLSDTLLSSLRDEKRDL
                     YSQINGLNDQKNVLNEKLSAANNFDNQLAGRIVHEALGNKSVIVFRTPDAKDDDVAAV
                     SKFIGQAGGTVTGTLSLTQEFVDANSAEKLRTVVNSSILPAGQQLSTKLVDQGSQAGD
                     LLGIALLINANPAVPPVDDPQRDVVLAALRDTGFITYQSGDHIGAANAALVITGGSLP
                     QDAGNQGVSVARFSAALAPHGSGALLAGRDGSATGGAAIAVTRADSGMSSAISTVDDV
                     DAAPGRITAVLGLHDLTNGGHVGQYGTGHGATAITVPQ"
     gene            7414..9165
                     /locus_tag="VFI55_10140"
     CDS             7414..9165
                     /locus_tag="VFI55_10140"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET7074424.1"
                     /translation="MRKHPQSATKHLFVSGGVVSSLGKGLTASSLGQLLTARGLHVTM
                     QKLDPYLNVDPGTMNPFQHGEVFVTEDGAETDLDVGHYERFLDRDLSGSANVTTGQVY
                     STVIAKERRGEYLGDTVQVIPHITDEIKGRILAMARPDADGNRPDVVITEIGGTVGDI
                     ESQPFLEAARQVRHDLGRENVFFLHVSLVPYLAPSGELKTKPTQHSVAALRSIGITAD
                     ALILRCDRDVPEALKNKIALMCDVDIDGIISTPDAPSIYDIPKVLHREELDAFVVRRL
                     NLPFRDVDWSEWDDLLRRVHEPHETVRIALVGKYVELSDAYLSVAEALRAGGFKHQAK
                     VEIVWVASDDCETPNGATAALGDVHGVLIPGGFGIRGIEGKIGAIRYARARGLPVLGL
                     CLGLQCIVIEAARSVGLTAANSAEFEPDTPDPVISTMADQEEIVAGEADLGGTMRLGA
                     YPAVLEPDSIVAQAYGSTQVSERHRHRYEVNNAYRERIAESGLRFSGTSPDGHLVEFV
                     EYPPDVHPFVVGTQAHPELKSRPTRPHPLFVAFVGAALDYKAGELLPVEIPEQSSNGN
                     QHRDSVRRPLPEPAARG"
     gene            9158..9781
                     /locus_tag="VFI55_10145"
     CDS             9158..9781
                     /locus_tag="VFI55_10145"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7074425.1"
                     /translation="MAEHVFETTASETLHTGKIFALRRDRVRMPGGNEVIREVVEHFG
                     AVAVVAMDDDGNVPMVYQYRHAFGRRLWELPAGLLDVHGEAAHLTAARELQEEAGLQA
                     GTWRVLVDLDSTPGFSDESVRVYLATGLTEVGRPEAHDEEADMTLHWYPLADAACKVL
                     NGEIVNAIAVAGILAAQAVTTGFAQPRPVDSPWQDKPKSFAARKAAQ"
     gene            9796..>10643
                     /locus_tag="VFI55_10150"
     CDS             9796..>10643
                     /locus_tag="VFI55_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET7074426.1"
                     /translation="MQLQGYLDHLTIERGVAANTLSSYRRDLRRYSKHLSDRGIHDLA
                     KVGEDDVSEFLVALRRGDPDSGAMALSAVSAARALIAVRGLHRFAAAEGLAELDVARA
                     VRPPTPGRRLPKSLTIDEVLALLEGAGGDSPSDGPLTLRNRALLELLYSTGSRISEAV
                     GLDVDDIDTKARSALLHGKGGKQRLVPVGRPAVQALDAYLVRGRPDLARRGLRTRATP
                     AIFLNARGGRLSRQSAWQVLQDAAERAGITSGVSPHMLRHSFATHLLEGGADVRVVQE
                     LLGHASV"
BASE COUNT         1740 a         3508 c         3703 g         1692 t
ORIGIN      
        1 agccgcagcg gctcgacggc agcgcggttg ttctacgcct acgcggacac tctgctggcg
       61 ctgggccgca acgatgaggc gctgcactgg ttcctgcgag ccgcggccgc cgacgtcgaa
      121 ggcgtcaccg acgccgaaga tcgggcggcc gagttgggcc cagcagacaa agaatgaaaa
      181 cccttgcgca ggaatatgac tgcctgctga tagacctgga cgggacggta ttccgcggcg
      241 cccagcccac caccggcgcg gtggcgtcgc tggaggaggt gcacagccgc aagctgtacg
      301 tcaccaacaa cgcctcccgc agcgccgacg aggtggcgtc gcacctgcgc gagctcggct
      361 tcaccgtcag cggcgcagac attgtcacca gtgcccagag cgcggcccgg ctgctcgccg
      421 atgagctacc ggcgcagtcc cgggtgctga tcgtgggtac cgaagccttg gccaacgaga
      481 tcgccgccgt cgggctgcgc ccggtccgcc gctacgacga ccacccggtc gcagtcgtgc
      541 agggtctgtc cacgaccatc ggctggcccg acctggcgga ggccgcgctg gccatccggg
      601 ccggcgcgct gtgggtggct gccaacgtcg accccacctt gcccaccgaa cgcggcctgc
      661 tgccgggcaa tggatcgctg gtggcggcgt tgcggactgc caccggagcc gaaccgcggg
      721 tggcgggcaa accggcgccc acgctgtttc aagaagcggc ggcccggggc gactttcgcg
      781 cgtcgctggt catcggtgac cggttggaca ccgacatcga gggcgccaac gccgccaagc
      841 tgcccagcct gatggtgctc accggggtca acaccgcgtg ggacgcggtg tatgcggaat
      901 ccgcccgtcg gcccacctac atcggtcacg acctgcgctc cctgcaccag gacgggcggt
      961 tgctcgcggt ggggccgcag ccgggctggc atgtcgacgt cggtggccag gcggtagccg
     1021 tcagcgccaa cggcgccgac gacggggacg ggctgtcgat cgtccgcgcc gtcgccgccg
     1081 cggtgtgggg agcgccgggc gccgacgcgc aggggcggcc ggtgcgcatc gaggccgccg
     1141 acgaccgggc gcgcgaggcg ttgcggcgct gggctctggt gcacagcgac tgagccatga
     1201 ctagcgtagg aactcgatga atatcgatcc cgatcagctt cgcgctgaaa tcgactccct
     1261 gattgcccag ctgccagagg tcggggaccc ggaaaacccc gaaagcgagc cgtccctcgc
     1321 ccggctcgaa gagatagcac gccgcctttc cgaggcgcac gacgtgctgc tgcaggcgct
     1381 ggagtcggcg gagaagggct gagtgcgacg gtggcacgac gcgcccgcgt tgacgccgag
     1441 ctcgtgcgac gcgggctcgc ccgatctcgg cagcaggccg ccgagttgat cggcgccggg
     1501 aaggtgacca tcgacggcct gccggcacgc aaacccggca cggccgtcgc gctcaccgca
     1561 gcactgaccg tcgcggccga tggcgaacgc agctgggtgt cacgtggagc gcacaaactc
     1621 atcggcgccc tcgatgcctt cgggatttcc gtcgccggcc gccgctgcct ggacgccggt
     1681 gcatcgaccg gtgggttcac cgaggtgctg ctggatcgcg gagccaccga agtggcggcc
     1741 gccgacgtgg gatacggcca gctggcgtgg tccctgcgca gcgacgcgcg ggtggtcgtc
     1801 gtcgaacgga ccaacgctcg tgatctgtcg cccgaggcga tcggcgggcc ggtcgacgtg
     1861 gtggtggctg acctgtcgtt catctcgctg gccaccgttt tgcccgcgct gattggatgc
     1921 gctttgccgg gcgcggatat cgttcccatg gtgaagccgc agtttgaggt ggggaagggc
     1981 caggtcggtg ccggcggggt ggttcacgac cccggtcttc gtgccgacgc ggtgctggcc
     2041 gtcgcgcggc gtgccgggga gctgggctgg cacaccgtcg acgtcaccgc cagcccgcta
     2101 ccgggaccgt cgggcaatgt cgagtacttc ctgtggttgc gcgccctgac ggaacggccc
     2161 ctgtcggatg aagggctgac caacgcggtg caccgcgctg ttcgggaggg cccgcagtga
     2221 ctccgaaaac gaccgcgcca cgcaccatcc tgttggtggt ccacaccggc cgggatgaag
     2281 cgaccgagac cgcgcggcgc gtggagaagg tgttgggcga caacgggatt gcgcttcgcg
     2341 tgctatccgc cgaggccgtc gaccgcggtt cgttgcacct ggcccccgac gacatgcgcg
     2401 cgctgggcgt agacatccaa gtggtcgacg ccgaccggca cgccgccgag ggctgcgagc
     2461 tggtgctggt tctcggtggc gacggcacct tcttgcgggc ggctgagttg gcccgcaacg
     2521 caggcattcc ggtgctgggc gtcaacctgg gccgaatcgg tttcttggcc gaggccgagg
     2581 cggaggccat cgacctggtg ctcgagaacg tcatcacccg cggctaccgg gtcgaggagc
     2641 gcctgacgct ggacatcatg gtgcgcgacg ggggccgcgt catcgagcgg ggctgggcgc
     2701 tcaacgaagc cagcctggaa aagggtccac ggctgggcgt gttgggggtg gtcgtcgaaa
     2761 tcgacggccg cccagtgtca acgttcggct gcgacggggt gttggtgtcc acgccgaccg
     2821 gatccaccgc ctacgcgttc tccgcgggcg gcccggtgtt gtggccggac ctcgaagcga
     2881 tcctggtggt acccaacaac gctcacgcgc tgttcggtcg gccgatggtc accagtccgg
     2941 atgccacgat cgcgatcgaa atcgaggcga gcggaaacga cgcgctcgtg ttctgcgacg
     3001 gccgccgcga aatgctgtta cccgccggcg ggcggctcga ggtgacccgc tgtggcacac
     3061 cggtgaaatg ggcgcgcctg gacagcgcgc cgttcacgga ccgcttggtg cgcaagttcc
     3121 ggttgccggt gaccggttgg cgcgggaagt agcggcgctg gccggtgctg actgaatgct
     3181 gacggaaatt cgcatcgagt cgctgggcgc gatcagcgtc gcgaccgctg agtttgatcg
     3241 cgggttcacg gtgctcaccg gtgagaccgg caccggcaag accatggtgg tcactgggtt
     3301 gcacctgctc ggtggtgccc gcgccgacgc aacgcgggtg cggtcgggag ccgaccgcgc
     3361 aaccgtcgaa ggtcgtttca ccacaaccga tctcgacgac gccgcggccg ctcagctcga
     3421 cgcgatgttg gacgcgtcgg gcgcggagcg cgatgaggac ggcagcgtga tcgcgttgcg
     3481 ctcggtcagc cgcgacggcc cgtcgcgcgc ctatctgggc ggtcgcagcg tgcccgccaa
     3541 gtcgctcagc ggattcaccg ccgagcttct caccctgcat gggcaaaatg accagcttcg
     3601 gttgatgcgg cccgaggagc agcgcggcgc gctggaccgc ttcgcgggcg ccggtccggc
     3661 gtacgagcgc taccgaaaac tgcgcgacgc ctggttgtcg gcgcggcgcg acctcatcga
     3721 ccgccgcaac cgatcccgcg aactcgccca ggaggcggac cggttgaggt tcgcgctcaa
     3781 cgagatcgat accgccgaac cgcaacccgg ggaagacgat gcgctggtcg ccgacatcct
     3841 gcggctctcc gagctggaca cgttgcgcga ggccgccgca atcgcacgcg cgtcgttgtc
     3901 cacggacgat gcagacgtct ccggcctcag cgctaccgac agcctgggga aagccagggc
     3961 cgcactggaa tccaccgatg atgtgacgct gcaggcgttg gggcgccaga tcggcgaagc
     4021 gctgaccgtc gtggtcgatg cggcccgtga gctcggcggc tttctggacg acctgcccgt
     4081 cgatgccagc gcgctggaat ccaagctggc ccgtcaggcc caactgcgcg cgctgacccg
     4141 taagtacgcg gccgacatcg atggggtgat gcggtgggct caggaggctc gggagaaact
     4201 ggcgcaactc gacgtctcgc cagaaggatt ggcgggcctg caatctcggg ttgatgagct
     4261 ggaacgcgaa ttgtctgagg ccgcagtcga tctcagcaag atcaggcgca aagcggccaa
     4321 gcgactgggc aaagaggtga ccgcggagct gtccggtctg gcgatggccg atgccgaatt
     4381 caccatcgac gtgagcacgg atatcgcgac cgggcaggat gattccgccg tgctcacgct
     4441 gccctccggt gaactggccc gggccggcgg cgacggcatc gaccaggtcg agttcggttt
     4501 cgcggcgcac cgcggtatga cggtgttgcc gctggccaag agcgcgtccg gaggcgagct
     4561 gtcccgcgtg atgctggccc tggaagtggt gctggccacg tcgcgaaagc aggccgctgg
     4621 aaccacgatg gtgttcgacg aggtcgacgc cggtgtcggc ggctgggccg ccgtgcagat
     4681 cgggcgccgg ctggcccggc tggcccgcac ccatcaggtc atcgtggtca cgcatttgcc
     4741 acaggtcgcg gcgtacgccg acgtgcatct ggtggtgcac agcggcggtc agggggcagg
     4801 tcggagctcc ggtgccagcg gcgtgcggcg ggtggccggc gacgagcggg tggccgagct
     4861 ggcgcggatg ctggccggcc tgggcgaatc cgacagcggc cgcgcacacg cccgtgaact
     4921 gctcgacgcg gcgcagaagg atgagatcta aacatctggg cacgctcgca ccaccgatag
     4981 cgcataacgc tgccccgtgc gcccgtgcga caaaatcgag gcccactttt aatccacctg
     5041 tgacaggtgt gactctagat aacttatgag gctgatgtta cggcgcgcct ccccgcctac
     5101 ctcggtcttc accgacagaa tcgcccccat gaagatgtca gggctgctat cgcgtaacag
     5161 caaccggccg ggcctcaccg gcaccgcacg ggtggaccgg aatatcgacc gactgctgcg
     5221 cagggtctgc cccggcgaca tcgtggtgct cgacgtcttg gatctggacc gcatcaccgc
     5281 ggacgcgctg gtggacgcag acatcgccgc cgtcgtcaac gcgtcgccgt ccgtatcggg
     5341 ccgctacccc aatctcgggc ccgaggtgct ggtcaacaac ggcgtcaccc tgatcgacga
     5401 gaccggaccc gacatcttca agaaggtcaa ggacggctcc aagattcgcc tgcacgaggg
     5461 cggggtgtac tccggtgacc gccggctggt ccgcggtact gagcgcaccg accacgacat
     5521 cgccgatttg atgcgcgaag ccaagagcgg actggcggcc cacctggagg ccttcgcggg
     5581 caacacgatt gagttcatca agagcgaaag cccgctgctg atcgacggca tcggtatccc
     5641 cgacatcgac gtcgacctgc gccgccggca tgtggtcatc gtcgccgacg aacccagcgc
     5701 cgccgacgat ttgcgatccc tcaaaccgtt catcaaggaa taccagccgg tgcttatcgg
     5761 cgtcggtacc ggtgcggatg tgctgcgcaa gggggggtat cgcccccaga tcatcgtcgg
     5821 cgaccccgac gagatcagca ccgaagccct caagagcggg gcccacgtcg tgctgcccgc
     5881 cgacgccgac ggccacgcgc ccggtttgga gcgaatccag gacctcggtg tcggggcgat
     5941 gaccttcccg gccgcgggtt cggcggtcga cctggcgctg ctgctggccg atcaccacgg
     6001 tgcctcgttg ctcgtgacgg ccggtcacac cgccaacatc gagaccttct tcgatcgcac
     6061 gcgcacgcaa agcaacccgt cgacattcct gaccaggctg cgcgtggggg agaaggtggt
     6121 ggacgccaag gctgtcgcca ccctgtaccg caaccacatt tcggcaggtg ccatcgcgct
     6181 gcttgcgctg acgatgctga tcgcggtcat cgtcgccctg tgggtgtcgc gcaccgacgg
     6241 tgtggtcctg cactggatcg tcgactactg gaaccacttc tccctcatgg tgcagaggtg
     6301 ggtcacctag cgttttccga ggagggtggg cctcatgatc tcgttacgcc aacacgcatt
     6361 gtcgttggcc gccgtctttt tggcgttggc cgtgggagtt gtgctgggtt ccggcttttt
     6421 gtcggacacc ctgctgtcca gcttgcgtga tgaaaagcgc gacctgtact cacagatcaa
     6481 cgggctcaac gaccagaaga atgtgctgaa cgaaaagctc tctgcagcaa ataatttcga
     6541 caaccagctg gccggccgaa tagtgcacga ggcgcttgga aacaaatcgg tgatcgtgtt
     6601 ccgcaccccg gacgcgaaag acgacgacgt ggcggcggtc tcgaaattca tcggccaggc
     6661 cgggggcacg gtgaccggaa cgttatcgct gacgcaggag ttcgtcgacg ccaactccgc
     6721 ggagaagctg cggacggtcg ttaactcgtc gatcttgccc gccggacagc aattgagcac
     6781 caagctggtg gaccaaggtt cgcaagcggg tgacctgttg ggcatcgcgt tgttgatcaa
     6841 cgccaacccc gccgtgccgc ctgtggacga cccccagcgg gacgtcgtgc tggcggcgct
     6901 gcgtgacacc ggcttcatca cctaccagtc gggcgatcac atcggcgcgg caaacgccgc
     6961 gctggtcatc accggcggct cgctgcccca ggacgccggc aatcagggcg tcagcgtggc
     7021 ccggttttcg gcggcgctgg cgccgcacgg ctcgggtgcc ctgctcgccg gccgcgacgg
     7081 ttccgcgacg gggggcgccg cgatcgccgt cacccgagcg gactccggca tgtcgtcggc
     7141 gatcagcacc gtcgacgacg tcgacgccgc gccggggcgg atcaccgcgg tgttgggcct
     7201 gcatgacctg accaacggtg gccatgtcgg gcaatacggc actggtcacg gggctacggc
     7261 gatcacggtg ccacagtagg gtccaggggc ccttagggcg tgttcgcggc accgccgctt
     7321 tcctggtgtt agggtgggtt tccgtgggtc ggcaggccca gctagttaac gctagacaaa
     7381 tcgatgccct gcccgtcttc acggaggtag ccagtgcgaa agcacccgca atccgccacc
     7441 aagcacctct tcgtcagcgg tggcgtcgtt tcgtctctgg gcaagggctt gaccgccagt
     7501 agcctcgggc agctgctgac ggcgcgcggg ttgcacgtga cgatgcaaaa gctcgacccg
     7561 tacctcaacg tcgacccggg caccatgaac ccgttccagc acggtgaggt cttcgtcacc
     7621 gaggacggtg ccgagaccga cctcgacgtc ggccactacg agcggttcct ggatcgcgat
     7681 ctgtccggct cggcgaatgt tacgaccggt caggtgtatt cgaccgtgat cgccaaggag
     7741 cgacgcggcg aatacctcgg tgacaccgtt caggtgatcc cgcacatcac cgacgagatc
     7801 aagggccgca tcctcgcgat ggctcgaccg gacgccgacg gcaaccgccc cgacgtcgtc
     7861 atcaccgaga tcggcggcac cgttggcgac atcgagtcgc agccctttct cgaggcggcg
     7921 cggcaggtgc gccacgacct gggccgtgaa aatgtgttct ttctgcacgt ctcgctggtg
     7981 ccttacctgg ctccctcggg tgagctcaag accaagccca cgcagcactc cgtggcggcg
     8041 ctgcgcagca tcggcatcac cgcggacgcg ctgatcctgc gctgcgaccg ggacgtcccc
     8101 gaagcgctga agaacaagat cgcgctgatg tgcgacgtcg atatcgacgg catcatctcc
     8161 accccggacg caccgtcgat ctacgacatt ccgaaggtgt tgcaccgcga ggaactcgac
     8221 gcgttcgtgg tccgccggct caacctgccg ttccgcgacg tggactggag cgaatgggac
     8281 gacctgctgc gcagggtgca cgaaccgcac gaaacggtac gaatcgccct ggtgggcaag
     8341 tatgttgagc tttccgacgc ctacctctcg gtcgccgaag cgctgcgggc cggcgggttc
     8401 aagcaccaag ccaaggtcga gatcgtctgg gtggcatccg acgactgcga gacccccaac
     8461 ggcgcgaccg cggcgctggg cgacgtgcac ggagtgctca tccccggcgg attcggcatc
     8521 cgcggcatcg agggcaagat cggtgctatt cggtacgcca gggcgcgcgg gttgccggtg
     8581 ctggggctgt gcctgggcct gcagtgcatc gtgatcgaag cggcccgctc ggttggtctc
     8641 acggcggcca actcggcgga attcgaaccg gacacaccgg atcccgtcat ctccacgatg
     8701 gccgatcagg aagaaatcgt ggccggcgaa gccgacctcg gcggcaccat gcgactgggg
     8761 gcctatcccg cggtgctcga gccggattcg attgtggccc aggcctacgg atccacacag
     8821 gtatccgagc gacaccggca tcgctacgag gtcaacaacg cttaccgcga acggatcgcc
     8881 gaaagcggac tgcgtttctc ggggacttcg cccgacggcc atctggtgga gttcgtcgaa
     8941 tacccgcccg acgtgcaccc gttcgtcgtc ggcacccagg cccaccccga gctgaaaagc
     9001 cggcccaccc ggccgcaccc gctgtttgtc gcgttcgtcg gggcggccct tgactacaaa
     9061 gcgggcgagt tgctgccggt ggagattccc gagcagtcgt ccaacggcaa tcagcatcga
     9121 gacagcgtcc ggcggccgtt acccgagccc gcggcccgtg gctgaacacg ttttcgagac
     9181 gactgcgtcc gaaaccctgc ataccggaaa gattttcgcg ctccggcgcg accgcgtgcg
     9241 gatgccgggc ggcaacgagg tcatcaggga ggtcgtcgag catttcggcg cggtggccgt
     9301 ggtggcgatg gacgacgacg gcaatgtccc gatggtctac caataccggc atgcgttcgg
     9361 ccgccggctg tgggaactgc cggccgggct gctggacgtc cacggcgagg ccgcccatct
     9421 caccgccgcc agggagctcc aggaagaggc cggcttacaa gccggcacgt ggcgggtgct
     9481 cgtcgatctc gactcgaccc cgggcttcag cgatgaatcg gtgcgcgtct atctggcgac
     9541 cggattgacc gaggtgggtc ggcccgaggc gcacgacgaa gaagccgata tgacgctgca
     9601 ctggtatccc cttgcggatg ccgcttgcaa ggtgctcaac ggcgaaatcg tcaatgccat
     9661 tgcggttgcg gggattctgg ccgcccaagc ggtgaccacc ggattcgcgc agccgcgccc
     9721 ggtggacagt ccatggcagg acaagccgaa atcgtttgcg gcgcgaaagg ccgcgcagtg
     9781 acggctgccc tggatatgca attgcagggc tacctagatc acctgaccat cgagcgggga
     9841 gtggcggcaa acacgctcag ctcctatcgg cgcgacctgc gccgctacag caagcatctg
     9901 tccgatcggg gcatccacga cctggccaag gtcggcgagg acgatgtcag cgagttcctg
     9961 gtggcgctgc ggcgcggcga tcccgactcc ggggccatgg cgctgtccgc cgtctcagcg
    10021 gcgcgggcgc tgatcgcggt gcgcggcctg caccggttcg cggccgccga ggggctcgcc
    10081 gagttggacg tggcgcgcgc cgtcaggcca ccgacgccgg ggcgccggtt gcccaaaagc
    10141 ctcacgatcg acgaggtgct ggcgctgctg gaaggcgccg gcggtgacag cccgagcgac
    10201 ggcccgctga cgctgcgcaa ccgggcgctg ctggaactgt tgtattccac cggatcgcgc
    10261 atctccgagg ccgtcggcct cgatgtcgac gacatcgata ccaaggccag gtccgcgttg
    10321 ctgcacggca agggcggcaa gcagcggctg gtgccggtgg ggcgcccggc cgtgcaggca
    10381 ctcgatgcct atctggtgcg gggccggccg gacttggcgc gccggggcct gagaacgcgc
    10441 gcgacgccgg cgatcttcct caacgcccgc ggcgggcggc tgtcccggca aagcgcgtgg
    10501 caagtgctgc aggacgccgc cgagcgcgcc ggaatcacat cgggtgtatc accgcacatg
    10561 ttgcggcatt cgtttgcgac tcacctgctg gagggcggcg ccgatgtccg cgtcgtgcag
    10621 gaattacttg gccatgcctc ggt
//
LOCUS       DASSGQ010000232         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_897335,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000232 DASSGQ010000000
VERSION     DASSGQ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897335"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VFI55_10155"
     CDS             complement(<1..382)
                     /locus_tag="VFI55_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein pafC"
                     /protein_id="HET7074427.1"
                     /translation="MTPVSDRLVRLLNMVPYFQANPRITRAEAAAELRVTGKQLEADL
                     NQLWMCGLPGYGPGDLIDFEFSGDTIEVTFSAGIDRPLQLTSPEATGLLVALRALADI
                     PGVVDPEAARSAIAKIAAAAGAAGH"
     gene            complement(379..1386)
                     /locus_tag="VFI55_10160"
     CDS             complement(379..1386)
                     /locus_tag="VFI55_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HET7074428.1"
                     /translation="MAISKVERLVNLVIALLSTRGYITAEKIRSSVAGYSDSPSTEAF
                     SRMFERDKNELRDLGIPLEVGRVSALDPTEGYRINRDAYALPPVELTPDEAAAVAVAT
                     QLWESPELITATQGALLKLRAAGVDVDPVDTGAPVAIASSAGVPGLRGSEGVLGILLS
                     AIDSRQVVQFPHRSSRAGPYTMRTVEPWGVITEKGRWYLVGHDRDRDATRTFRLSRIG
                     ADVTPIGAAGAVTVPRGVDLRKIVADTVSETPAGGQARVWAADGRATALRRAGRSIGA
                     RQLAGRDGEVIELDIGSTDQLARDITGNGADAVVLEPESLREDVLARLRAQAGDEERR
                     P"
     gene            complement(1594..>2597)
                     /gene="pafA"
                     /locus_tag="VFI55_10165"
     CDS             complement(1594..>2597)
                     /gene="pafA"
                     /locus_tag="VFI55_10165"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HET7074429.1"
                     /translation="YGCHENYLIVRAGEFSRISDVLLPFLVTRQVICGAGKVLQTPKA
                     ATFCLSQRAEHIWEGVSSATTRSRPIINTRDEPHADAEKYRRLHVIVGDSNMCETTTM
                     LKVGTASLVLEMIEAGVPFRDFSLDNPIRAIREVSHDVTGRRPVRLAGGRQASALDIQ
                     REYYSRAVEHLQTREPNAQVEQVVDLWGRQLDAVESQDFAKVDTEIDWVIKRKLFQRY
                     QDRYNMELSDPKIAQLDLAYHDIKRGRGVFDLLQRKGLAARVTTDEDIAEAVDTPPQT
                     TRARLRGEFISAAQAAGRDFTVDWVHLKLNDQAQRTVLCKDPFRAVDERVKRLIASM"
BASE COUNT          392 a          880 c          894 g          431 t
ORIGIN      
        1 cgtgcccggc cgcgcccgcg gccgcggcga tcttggcgat cgcgctgcgc gccgcctcgg
       61 ggtcgacgac gcccgggatg tctgcgagtg cgcgtagcgc gaccagcagt ccagtggcct
      121 cgggagaggt cagctgcaga ggccgatcga tgcccgcaga gaacgtcacc tcgatggtgt
      181 cgccggagaa ctcgaaatcg atgaggtcgc cggggccgta gccgggaagg ccgcacatcc
      241 acagctggtt gaggtcggcc tcgagctgtt tgccggtcac cctcagctcg gcggcggcct
      301 ccgcgcgggt gatccgcggg ttggcctgga aatacggcac catgttcagc aaccgcacca
      361 gacggtcgga cacgggagtc atgggcgccg ctcctcatcg ccagcttggg cccgcaaccg
      421 ggccaacacg tcctcgcgca gcgactctgg ctccagcacg acggcgtcgg cgccattgcc
      481 ggtgatgtcg cgcgccagct gatcggtgga tccgatgtcg agttcgatca cctcgccgtc
      541 gcggccggcg agttgccgcg caccgatcga ccgtcctgcg cggcgcagcg cggtggcccg
      601 tccgtcggca gcccacaccc gggcctggcc gccggccggt gtttcactga ccgtgtccgc
      661 cacgatcttg cgcaggtcca caccgcgggg cacggttacc gcaccggccg caccgatcgg
      721 tgtgacgtcg gcacctatcc gtgagagccg gaaggtgcgg gtggcgtcgc ggtcacggtc
      781 gtggcccacc agataccagc ggcccttctc ggtgatcacg ccccacggct cgacggtgcg
      841 catcgtgtag ggcccggccc gcgacgaccg gtgcgggaac tgcaccacct ggcgggaatc
      901 gatggccgac aacaggattc cgagaacacc ctccgacccg cgcaagcccg gcacaccggc
      961 cgaagacgcg atggccaccg gggcccccgt gtcgacggga tcgacatcga cccccgcggc
     1021 gcgcagcttg agcaacgcgc cctgggtggc ggtgatcagc tcgggcgact cccacaactg
     1081 cgtggcgacc gcgaccgcgg ctgcctcgtc cggagtcagc tcgaccggcg ggagcgcata
     1141 ggcgtcgcgg ttgatccggt agccctcggt ggggtccagc gccgacaccc tgccgacctc
     1201 gagggggatg ccgaggtcac gcagttcgtt cttgtcgcgc tcgaacatcc gcgagaacgc
     1261 ctcggtgctg gggctgtcgg agtaccccgc cacgctggac ctgatctttt cggcggtgat
     1321 gtagccgcga gtggacagca gagcaatgac aaggtttacc agccgctcga ctttcgagat
     1381 cgccatttgc accaggttat tagatggccc tcgctccgcg cttccaccgg ctagccgcgt
     1441 ctcctaatca cagctgccac atgggtccct cggcccgggg gcccaccgcc tcgcgaaact
     1501 tgatagcgcc cgcgctagcg cgtttggcat tcgaccgcag gcggccacgc cggtgctggt
     1561 gtgactaatg cgaccagtgt tgaaccaaca cggctacatg ctggcgatca gccgcttgac
     1621 ccgctcgtcg accgctcgga acgggtcctt gcacagcacg gtgcgctgcg cctggtcgtt
     1681 gagcttgagg tgcacccagt cgacggtgaa gtcgcgccct gccgcctggg cggcgctgat
     1741 gaattccccg cgcagcctgg cgcgagtggt ctgcggcggg gtgtcgacag cctcggcgat
     1801 gtcctcgtcg gtggtgaccc gagccgccag gcccttgcgc tgcagcaggt cgaagacgcc
     1861 gcgcccgcgc ttgatgtcgt ggtatgccag gtccaactgg gcgatcttcg ggtcggaaag
     1921 ctccatgttg tagcggtcct ggtagcgctg gaacagcttg cgcttgatca cccagtcgat
     1981 ttcggtgtcc accttggcga aatcctggct ctcgacggcg tcgagttggc ggccccacag
     2041 gtcgaccact tgctcgacct gcgcgttggg ctcccgggtc tgcagatgct cgacggcgcg
     2101 gctgtagtac tcgcgctgga tgtccagcgc gctggcctga cgcccgccgg ccagccgcac
     2161 cggccggcga ccggtgacgt cgtggctgac ctcgcggatg gcacgaatgg ggttgtccag
     2221 ggagaagtcg cggaagggaa cgccggcttc gatcatctcc agcaccagcg acgcggtgcc
     2281 caccttgagc atggtcgtcg tctcgcacat gttggagtcg ccgacgatga cgtgcagccg
     2341 ccggtacttt tcggcgtcgg cgtgcggctc gtcgcgggta ttgatgatgg ggcgtgatcg
     2401 ggtcgttgcg ctcgagacac cctcccagat gtgctcggcg cgttgggaaa ggcagaacgt
     2461 ggcggccttg ggggtctgca gcaccttgcc ggccccgcag atcacctggc gagtgaccag
     2521 gaacggcagc agcacatcgg agatccggga gaactcgccg gcgcgcacga tcaggtagtt
     2581 ctcgtggcag ccatacg
//
LOCUS       DASSGQ010000233         8714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_502977,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000233 DASSGQ010000000
VERSION     DASSGQ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8714
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502977"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /gene="egtA"
                     /locus_tag="VFI55_10170"
     CDS             complement(<1..1034)
                     /gene="egtA"
                     /locus_tag="VFI55_10170"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis glutamate--cysteine
                     ligase EgtA"
                     /protein_id="HET7074430.1"
                     /translation="MTSAAVTAAAESEMADSSAVAQYIADSCLVDAPLGSVGLELEGH
                     CHDPADPYRRPSWAEISAVIDFLPTLPCGSKVTVEPGGAVELSGPPVVGAEAAIDAMN
                     RDQAVLRSAFADAGLGLVFLGADPLRPPKRINPGERYRAMEQFFGASRSGAAGAAMMT
                     STASVQVNLDAGPRDGWAARVRLAHALGPTMIAIAANSPMLGGEFTGWVSTRQRVWGQ
                     MDSARCGPILGASGDDPGTDWARYALKAPVMLVHNRDASDLTAVTHWVPFADWVDGRV
                     MLGDRRPTIADLEYHLTTLFPPVRPRQWLEIRYLDTMPDAVWPAVVFTLTALLDDPVA
                     ADIAAEVVEPV"
     gene            complement(1202..1849)
                     /locus_tag="VFI55_10175"
     CDS             complement(1202..1849)
                     /locus_tag="VFI55_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HET7074431.1"
                     /translation="MRISAAAASVGLALGGALSIPVAAANPSEPGVVSYAVLGKGSVG
                     NIVGGPMGWESVFNQPFQGFWVDLPVCNNWADIGMSEVYDDPDLASFNGAITQTSATD
                     QTHYVKQALGVFATNDAAGRAFHRIVDRTVGCSGQTTAMHLDNGITQVWSFDGGPATG
                     TDANWTKQEAGTDRRCFDQTRLRENVLLQAKVCQSGNAGPAVNVLAGAMQNALGQ"
     gene            complement(1864..2292)
                     /locus_tag="VFI55_10180"
     CDS             complement(1864..2292)
                     /locus_tag="VFI55_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HET7074432.1"
                     /translation="MTIEVVYTTESTATGGGRDGHVKSADGRIDLDTRPPKVMGGSGE
                     GTNPEQLFSAGYAACFLGALRLVAGKAKVKLDDATDVTVQIGFGKDSAGGFGITGKIV
                     GYLPGLDQSAADELVEQAHQVCPYSKATRGNIDVDLAAKV"
     gene            complement(2326..3777)
                     /locus_tag="VFI55_10185"
     CDS             complement(2326..3777)
                     /locus_tag="VFI55_10185"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HET7074433.1"
                     /translation="MTERYTTTDAGIAAPSDDQSLTIGPDGPILLQDHYLIEQMAMFN
                     RERIPERQPHAKGGGAFGHFEVTNDVSKYTRAAVFQPGTKTDMLVRFSTVAGERGSPD
                     TWRDPRGFALKFYTSEGNFDMVGNNTPVFFMRDPLKFQHFIRSQKRMQATNLRDHNMQ
                     WDFWSLSPESAHQVTWLMGDRGIPKNWRHMNGYSSHTYSWINAAGEIFWVKYHFITDQ
                     GIDFLTQQDADRLAGEDGDYHQRDLYEAIEGGDFPSWTLKMQIMPFEEAKTYRFNPFD
                     LTKVWPHSDYPLIDVGKMTLDRNVTDYHTEIEQAAFEPNNLVPGTGLSPDKMLLARGF
                     SYADAHRARLGTNYKHIPVNSPHVEVNSYSKDGAMRMKNASDPVYAPNSYGGPQADPA
                     RAAEVRWMADGDMVRAAYTLRADDDDFGQAGTMVREVLDDAARDRLAHNIIGHVSKGV
                     REPVLSRVFEYWRNVDPDLGKLVEEGVRANLGQ"
     gene            complement(3966..4427)
                     /locus_tag="VFI55_10190"
     CDS             complement(3966..4427)
                     /locus_tag="VFI55_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074434.1"
                     /translation="MSETPDPATRPTPVAATPPPRAYEPVKPPRVYVAAAWVVIVAGI
                     LFILATVFFAGAMVVGFNHHCHDRYHHDMMYKPGGPWSPGGGPGQFGEPYGPGMAPPY
                     GPPPGFGPGGPGGPPPSFAPGFGPGGQGGYSQSPVTSPPAPAPSTPGNPRP"
     gene            complement(4573..5016)
                     /locus_tag="VFI55_10195"
     CDS             complement(4573..5016)
                     /locus_tag="VFI55_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074435.1"
                     /translation="MRSSTATAPAPVETEAAPHRTPRVFQIAAWVAIVAGIVFIVAVV
                     FFSGFILGKHSGHHGGHHHKHHAMMHPHRFGGPGGPGGPGGFGGPGGPGGPGGGPSAT
                     PSPGPTTVRPSAGPEQVPSSLFRSATALPSAAVPGGAPTVVVLAG"
     gene            complement(5149..6246)
                     /locus_tag="VFI55_10200"
     CDS             complement(5149..6246)
                     /locus_tag="VFI55_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HET7074436.1"
                     /translation="MCLLAPHCLSVARADPPAPAPEPILPPLAVGQVLRIGPTAGTGT
                     ATTDYGIGATDLCEFVEFPTELLQVCGDSFAGQGVGFGGWYSPVALHVDSASVDDPGG
                     VRYTGVTGIGKPLLADPTPAGDSQLPAGVVQINRRNYLMVTTTRNLVPQSSRLVAPEP
                     ARGNWQTVAGSRRDASYQGGSQTQISGYYDPIPTADSVTGWVYIVANSFTRRDPLLLY
                     RATPQTFTDRSRWQGWATGPEGGWNKPPTPLWPDQVGEMCIREIDGKAVLSYFNATTG
                     NMEVRVANDPTSLGTAPVTTVVQHDEWPDPAESLPPPFDNRLAQPYGGYISPGSTLDE
                     LRIFVSQWDTRARVSAPYRVIQFGVNPFKPD"
     gene            complement(6302..7369)
                     /locus_tag="VFI55_10205"
     CDS             complement(6302..7369)
                     /locus_tag="VFI55_10205"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET7074437.1"
                     /translation="MGADRLAIGVVGATGQVGQVMRTLLEERDFPAAAGDGKVRFFAS
                     ARSQGRKLAFRGQEIEVEDAATADPTGLDIALFSAGKTMSLVQAPRFAAAGVTVIDNS
                     SAWRRDPDVPLVVSEVNFERDVGSAGSLKKGIIANPNCTTMAAMPVLKVLHDEARLVR
                     VVASTYQAVSGSGLAGVAELAEQARAVVGDAEELVYDGGALEYPAPKTYVAPIAFNVV
                     PLAGSLVDDGSGETDEDQKLRFESRKILGIPDLLVSGTCVRVPVFTGHSLSINAEFDR
                     PISPERARELLSGAPGVKVVEVPTPLAAAGVDESLVGRIRQDPGVPDGRGLALFVAGD
                     NLRKGAALNTVQIAELLAAQL"
     gene            complement(7372..8637)
                     /locus_tag="VFI55_10210"
     CDS             complement(7372..8637)
                     /locus_tag="VFI55_10210"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET7074438.1"
                     /translation="MALVVQKYGGSSVADADRIRRVAERIVATKKLGNDVVVVVSAMG
                     DTTDDLLDLAQQVCPAPPPRELDMLLTAGERISNALVAMAIESLGAQARSFTGSQAGV
                     ITTGTHGNAKIIEVTPGRLRTALEEGRIVLVAGFQGVSQSTRDVTTLGRGGSDTTAVA
                     LAAALGADVCEIYTDVDGIFSADPRIVPNARKLDTVTFEEMLEMAACGAKVLMLRCVE
                     YARRYNIPVHVRSSYSDRPGTVVIGSIKDKAMEDPILTGVAHDRSEAKVTIAGIPDLP
                     GYAAKVFRAVADADVNIDMVLQNVSKVEDGKTDITFTCSRDSGPTAVAKLDSLREEIG
                     FTQLLYDDHIGKVSLIGAGMRSHPGVTATFCEALAAVGVNIELISTSEIRISVLCRDT
                     ELDKAVVALHEAFRLGGEETATVYAGTGR"
BASE COUNT         1340 a         2968 c         2934 g         1472 t
ORIGIN      
        1 accggttcga cgacctcggc cgcgatgtcg gcggcgaccg gatcgtcgag cagcgccgtg
       61 agcgtgaaca ccacggcggg ccagacggcg tcgggcatgg tgtcgagata gcggatttcc
      121 agccactgcc tcgggcgtac cggcgggaac agtgtggtca ggtgatactc caggtcggcg
      181 atcgtcgggc gacgatcgcc gagcatcacc cggccgtcca cccagtcggc gaagggcacc
      241 caatgcgtga ccgctgtgag gtccgaggcg tcccggttgt gcaccagcat caccggcgcc
      301 ttgagcgcat agcgcgccca gtcggtgccc gggtcgtcgc cgctggcacc caggatgggc
      361 ccgcaccgcg ccgagtccat ctgtccccac acccgctgcc gggtggagac ccagccggtg
      421 aactcgccgc ccagcatcgg ggagttggcg gcgatcgcga tcatcgtggg ccccagagcg
      481 tgcgccagcc gtacccgcgc cgcccacccg tcccgcggac cggcgtccag attgacctgg
      541 accgatgcgg tggacgtcat catcgccgcg ccagcggccc cggagcggct ggcgccgaag
      601 aactgttcca tggcgcggta acgctcgccc gggttgatgc gcttgggtgg ccgcagcgga
      661 tcggccccga ggaagaccag ccccagcccg gcgtcggcga atgccgaccg cagcacggcc
      721 tggtcgcgat tcatcgcgtc gatggcggcc tccgcgccga ccaccggcgg cccggacagt
      781 tcgacggcgc cccctggttc gacggtgacc ttgctgccgc agggcagcgt ggggagaaag
      841 tcgatgaccg cgctgatttc cgcccagctc ggcctgcggt acgggtcggc cgggtcgtgg
      901 caatgccctt ccagctccag gccgaccgac cccagcgggg cgtcgaccag acagctgtct
      961 gcgatgtatt gcgccacggc agaggagtcg gccatctcgg attcggcagc ggcggtgaca
     1021 gcggcggacg tcatatcacg atcccttcgg gccccggcat gcccgggcat gtcgtgtgga
     1081 ccgcttgttg cgcagctaac gaagaactaa tcgccactac ttcatcttgc agatcgcccc
     1141 gacatattcc cgttcccgcc ggattttccc ctcacggcct gtccgagtac ccggctccac
     1201 gctactgacc cagcgcgttc tgcatcgccc cggccagcac attcaccgcg gggcccgcgt
     1261 tgccagactg gcagactttg gcctgcagca acacgttttc gcgcagcctg gtttgatcga
     1321 agcaccgccg atcggtgccg gcttcctgct tggtccaatt cgcatcggtg cccgtcgccg
     1381 ggccgccgtc gaacgaccac acctgcgtga tgccgttgtc caggtgcatc gccgtggtct
     1441 gtcccgagca tccgaccgtc cggtccacga tccgatggaa ggcccgtccg gcggcgtcgt
     1501 tggtggcgaa taccccgagc gcctgcttga cgtagtgggt ctggtcggtg gcggacgtct
     1561 gggtgatggc gccgttgaac gacgccagat cgggatcgtc gtacacctcg gacatgccga
     1621 tgtccgccca gttgttgcac accggcagat caacccagaa tccctggaac ggctggttga
     1681 acaccgactc ccaccccatc ggcccgccga cgatattgcc gaccgatccc ttgccgagga
     1741 ccgcgtagga caccaccccc ggctccgacg ggttggcggc cgcgaccgga atcgacagcg
     1801 cgcccccaag agccagaccg acgctcgccg ccgcggcgga gatccgcatc gcttccgacc
     1861 gcgctagacc ttggcggcga ggtcgacgtc gatgttgccc cgggtggctt tggaatacgg
     1921 gcacacctga tgggcctgct ccaccagctc gtccgccgcg ctttggtcca ggcccggcag
     1981 gtacccgacg atcttgccgg tgatcccgaa tccgcccgcg gaatccttgc cgaacccgat
     2041 ttgcacggtc acatccgtgg cgtcgtcgag cttgaccttc gccttgccgg ccaccaggcg
     2101 cagcgcaccc aggaagcagg cggcgtagcc ggcggagaac agctgctcgg ggttggtgcc
     2161 ctcgccgctg ccgcccatca ccttgggagg ccgggtgtcg aggtcgatgc ggccatccgc
     2221 cgacttcacg tggccgtcgc ggccgccgcc ggtcgctgtg ctttccgtgg tgtaaacgac
     2281 ttcaatggtc atgggctcga tcatgccagg cgcggtgccc gcctcctact gccccaggtt
     2341 ggcccggacc ccttcctcga ccaacttgcc gagatcgggg tcgacgttgc gccagtattc
     2401 gaacacccgc gacagcaccg gctccctgac gcccttggac acatgcccaa tgatgttgtg
     2461 cgccaagcgg tcacgggccg cgtcatcgag aacttcgcgg accatggtgc cggcctgtcc
     2521 gaagtcgtcg tcgtcggcac gcagcgtgta ggccgcgcgc accatgtcac cgtcggccat
     2581 ccagcgcacc tcggctgctc gcgccggatc ggcctgcggg ccgccgtagg agttgggcgc
     2641 gtacacggga tcggaggcat tcttcatccg catcgcaccg tctttggagt aactgttcac
     2701 ctccacgtgc ggcgaattga ccgggatgtg cttgtaattg gttcccagcc gggcccggtg
     2761 cgcgtcggcg taggagaagc cgcgggccag cagcatcttg tccgggctca agccggtgcc
     2821 agggacgagg ttgttgggct cgaaggccgc ctgctcgatc tcggtgtggt agtcggtgac
     2881 gttgcggtcc agcgtcatct tgccgacgtc gatcagcggg tagtcgctgt gcggccagac
     2941 cttggtcaga tcgaaggggt tgaaccggta ggtcttggcc tcctcgaacg gcatgatctg
     3001 catcttcagc gtccaactcg ggaagtcgcc gccctcgatc gcctcataga ggtcgcgctg
     3061 gtggtagtcg ccgtcctcgc cggccagccg gtcggcgtcc tgctgggtca ggaagtcgat
     3121 gccctgatcg gtgatgaagt ggtacttcac ccagaagatc tcgccggcgg cgttgatcca
     3181 gctgtaggtg tggctgctgt agccgttcat gtgccgccag ttcttcggaa tcccgcggtc
     3241 gcccatcagc caggtgacct ggtgcgccga ctccggcgaa aggctccaga agtcccactg
     3301 catgttgtgg tcgcgcaggt tggttgcctg catgcgcttc tgggaccgga tgaagtgctg
     3361 gaacttcagc gggtctcgca tgaagaacac cggcgtgttg ttgcccacca tgtcgaagtt
     3421 gccctccgac gtgtagaact tcagcgcgaa gccgcgcggg tcccgccagg tgtctgggct
     3481 gccgcgctcg ccggcgacgg tggagaacct gaccagcatg tcggtcttgg tgcccggctg
     3541 gaacaccgcg gcccgggtgt acttgctgac gtcgttggtc acctcgaaat ggccgaacgc
     3601 gccgccgcct ttggcgtgcg gctggcgttc cgggatccgt tcccggttga acatcgccat
     3661 ctgctcgatc agatagtggt cctgcagcag gatcggaccg tccgggccga tggtcaacga
     3721 ctggtcgtca ctgggcgcag cgatgccggc gtcggtggtg gtgtagcgct ccgtcatttc
     3781 gcgatctcct cgctaactgg gcttacttac cgaaaatcca agggcgaagg gcatttcgga
     3841 ttgaattttg gttggcgcca agcactttca tcgcgactgc gcgcgaatga aaggcactct
     3901 tcccagtatc cccctgctct ggaacgggtc aagaagacat aggcccgcta cgcatggcta
     3961 ccgagctacg ggcgcgggtt tcccggggtg ctcggcgccg gcgctggcgg gctggtcacc
     4021 ggcgactgac tgtagccacc ctggccgccg gggccgaagc ctggagcgaa gctcggcggg
     4081 gggccacctg gaccacccgg gccgaagccc ggcggcggcc cgtacggggg ggccatcccg
     4141 ggaccataag gctcgccgaa ctgccccggt ccgccgcccg ggctccaggg cccgccgggt
     4201 ttgtacatca tgtcgtggtg atagcggtca tggcagtggt ggttgaaccc cacgaccatg
     4261 gcgccggcga aaaagacggt ggcgaggatg aagaggatgc cggcgacgat gaccacccag
     4321 gcggcggcca cgtacaccct cgggggtttg accggttcgt acgcacgtgg cggcggagtt
     4381 gcggcgacgg gcgtcggccg cgttgcgggg tcgggtgttt cgctcatgtc ttgaatactg
     4441 ccccggtcac caaccggtct aacaggttcc cgcgagaaaa gttgcagtga attgcacccg
     4501 aaagcagtaa cccggcgctc gggtgagcgc cgggttaccg gtttgcgggt tgacggcccc
     4561 cgggtcctcc tattacccgg cgagcactac gactgttggg gccccgcccg gtacggcggc
     4621 cgacggcaag gcggtcgcgg agcggaagag cgaagacggc acctgctcgg ggccggcgct
     4681 gggacgcacc gtggtgggtc caggcgacgg ggttgccgat ggtccgccac cggggccacc
     4741 gggtccaccg gggccgccga aaccaccggg gccacccgga ccgcccgggc cgccgaatcg
     4801 gtgcggatgc atcatcgcgt gatgcttgtg gtggtgtccg ccgtggtgac cggagtgctt
     4861 gccgaggatg aagccggaga agaagacgac cgcgacgatg aagacgatcc cggcgacgat
     4921 cgcgacccag gccgcgatct ggaagaccct gggggtccgg tgtggcgctg cctctgtttc
     4981 gacgggcgcc ggcgcagttg cggtagagga ccgcacagtg ggggtgtcag atgtttcact
     5041 catgagacac atgatgcggg ggtaaacaat ggtgatggct atgcggtact taagtaccag
     5101 ctatgagctg aaaatcgccc tcacctggtc aaagccccaa ccggagtcct aatccggctt
     5161 gaacgggttg accccgaact ggatcacccg gtacggcgcg ctcacgcggg ctctggtgtc
     5221 ccactggctc acgaatatcc gcagctcatc gagggtggac ccgggcgaga tatacccgcc
     5281 atagggttgt gcgagccgat tgtcgaacgg gggcggcagg ctttccgccg gatcgggcca
     5341 ctcatcgtgt tgcaccaccg tcgtcaccgg cgcggtgccc agggacgtcg gatcattggc
     5401 cacccggacc tccatgttgc ccgtggtggc gttgaaatac gacagcaccg ccttgccgtc
     5461 gatctccctg atgcacatct cgcccacctg gtcgggccac aggggcgtcg gcggcttgtt
     5521 ccacccgccc tcgggcccgg tcgcccagcc ctgccatcgg gaccggtcgg tgaacgtctg
     5581 cggggtggcc cgatacaaca gcagcgggtc cctgcgggtg aagctgttgg ccacgatgta
     5641 cacccagccg gtcaccgaat cggccgtggg gatcgggtcg taatacccgc tgatctgcgt
     5701 ctgcgagccg ccctggtagg acgcgtcgcg ccgcgacccc gcgacggtct gccagttgcc
     5761 gcgggccggc tccggcgcga ccagccgtga gctctgcggc accaggttgc gcgtcgtcgt
     5821 caccatcagg tagttgcgcc ggttgatctg caccaccccg gcgggcagct gggagtcccc
     5881 ggccggtgtg gggtcggcca gcaacggctt gccgatcccc gtgacgccgg tgtagcgcac
     5941 cccacccgga tcgtcgaccg acgcggagtc gacgtgcagc gcgaccggcg agtaccaacc
     6001 accgaatccg acaccctggc cggcgaagct gtcgccgcag acctgcagca gctcggtggg
     6061 gaactccacg aactcacaca ggtcggtcgc gccgattccg tagtcggtgg tggctgtccc
     6121 ggtgccggcg gtggggccga tccgcaacac ctggcccacc gccagcggcg gcaggatcgg
     6181 ctccggggcc ggggccgggg gatcggctcg cgcgaccgaa agacagtgcg gcgcaagcaa
     6241 acacaggacc gacgcagcaa gtatcgagcg ttgcgcgggc cgggcggatt tcacccgcgg
     6301 gtcacagctg cgcggccagc agctcggcaa tctggacggt gttcagcgcc gcgcccttgc
     6361 gcaggttgtc gcccgcgacg aacagcgcca ggccgcgccc gtcgggcacc ccggggtcct
     6421 gccggatgcg cccgaccaat gattcgtcga caccggcggc cgccagcggc gtcgggacct
     6481 ccaccacctt gacacccggc gcgccgctga gcagctcacg ggcgcgctcg ggcgatatcg
     6541 ggcggtcgaa ctcggcgttg atcgacagcg agtgcccggt gaacaccggg acgcgcacgc
     6601 aggtgccgct gaccagcagg tccgggatcc cgaggatctt gcggctctcg aagcgcagct
     6661 tctggtcctc gtcggtctct ccggacccgt cgtccaccag tgacccggcc agcggcacca
     6721 cgttgaacgc gatcggcgcc acgtaggtct tcggcgccgg gtattccagc gcaccgccgt
     6781 cgtaaacgag ctcttcggcg tcgccgacca cggcgcgcgc ctgctcggcc agctcggcca
     6841 ccccggccag tccgctgccg gacaccgctt gataggtcga ggcgaccacg cgcaccagcc
     6901 gggcctcgtc gtgcaggacc ttgagcaccg gcatcgcggc catggtggtg cagttcgggt
     6961 tggcgatgat gccctttttg agggatcccg cgctgcccac gtccctctcg aagttcacct
     7021 cggacaccac gagcggcaca tccgggtccc tgcgccaggc cgacgagttg tcgatcaccg
     7081 tcaccccggc ggcggcgaac cgcggcgcct gcaccagcga catcgtcttg ccggccgaga
     7141 acagcgcgat gtccaggccg gtcgggtcgg ccgtcgcggc gtcttcgacc tcgatctcct
     7201 gaccgcggaa ggcgagcttg cggccttgcg accgggccga cgcgaagaac ctcactttcc
     7261 catcaccagc agccgccggg aagtcacgct cctcgaggag cgtgcgcatc acctgaccca
     7321 cctgaccggt ggcgcccact acacctatcg cgagcctatc ggcgcccata tctatcgccc
     7381 cgtccccgca tacaccgtcg ccgtctcctc gccgccaaga cggaacgcct cgtgcaatgc
     7441 cacgacggcc ttgtccagtt cggtgtcgcg gcacagcacc gagatgcgaa tctcggaggt
     7501 cgagatgagc tcgatgttga cgccgacggc ggccagcgcc tcgcagaagg tggcggtgac
     7561 cccagggtgg ctgcgcatgc cggccccgat cagcgacacc ttgccgatgt ggtcgtcgta
     7621 gagcagctgg gtgaacccga tctcctcgcg cagcgagtcc agcttggcca ccgcggtggg
     7681 cccgctgtcg cgagagcagg tgaaggtgat gtcggtcttg ccgtcttcga ccttggagac
     7741 gttctgcagc accatgtcga tgttcacgtc ggcgtcggcg acggcccgga acaccttggc
     7801 cgcgtacccc gggaggtccg gtatcccggc gatggtcacc ttcgcctcgc ttcggtcgtg
     7861 tgcgactccg gtcaggattg ggtcttccat ggctttgtcc ttgatcgatc cgatgacgac
     7921 ggtgcccgga cggtccgaat acgacgaccg gacgtggacc gggatgttgt agcggcgggc
     7981 gtattccacg cagcgcagca tcagcacctt ggcgccgcag gccgccatct cgagcatctc
     8041 ctcgaacgtc accgtgtcca gcttgcgggc gttggggacg atccgcgggt ccgcgctgaa
     8101 gatgccgtcc acgtcggtgt agatctcgca gacgtcggcg ccgagggcgg cggccagggc
     8161 gaccgccgtg gtgtccgagc cgccgcgccc cagcgtggtg acgtcgcggg tgctctggct
     8221 gaccccctgg aatccggcga ccagcacgat ccggccttcc tcgagcgctg tgcggagccg
     8281 tcccggggtg acctcgatga ttttcgcgtt gccgtgggtg ccggtggtga tcacgccggc
     8341 ctgcgaaccg gtgaacgagc gggcctgcgc gccgagcgac tcgatggcca tggccaccag
     8401 cgcattggag atgcgctcac cggcggtcag cagcatgtcg agttcccgtg gcggcggcgc
     8461 cgggcacacc tgttgcgcca gatccagcag gtcgtcggtg gtgtcaccca tcgcggacac
     8521 caccacgacg acgtcgttgc ccaacttctt ggtcgcgacg atgcgctccg caacgcggcg
     8581 gatccggtcg gcgtcggcca ccgaggatcc gccgtacttt tgcacaacga gcgccactgt
     8641 cgctccgcct gcccttctga gtgatcgccg gggtttgagt ccacaacaga atacgtgccg
     8701 ccgcatcccc gttt
//
LOCUS       DASSGQ010000234         9719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_836871,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000234 DASSGQ010000000
VERSION     DASSGQ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9719
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836871"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..1857)
                     /locus_tag="VFI55_10215"
     CDS             complement(181..1857)
                     /locus_tag="VFI55_10215"
                     /inference="COORDINATES: protein motif:HMM:NF013127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HET7074439.1"
                     /translation="MSFVIATPDLVESAAQNLAGVRSSLAEAAATAAGPTTGIAVAAQ
                     DEVSIAISSMFGNFGQEFQALSAQAQAFHEQFVGLLNAGAGAYLSTEAANAGQGLLGG
                     GVLGSVGQNLGGAFAGGGTAIGQFAGNIGNGLNGAATALQNGSFGALVSAQIQVDAQA
                     ISAGVAGAPAAAPTVIQGINEFWAGVAAPYQALAANTQTNLQAIGSTIAANPFPVLNQ
                     LSINQQGYARTFFNGVALDLQGFPANVPANVQLAIQGASTFNPGALLQQFVNGQIATG
                     QTTSTSLQNAAGDFSAGLPTFSAGVQTAFHDLLAGNPVGAYGDLNQALVNAFLPGFNS
                     IQVAPGASGLGEFLVTPMGPLGDLGPILDIPGQMAQNFVTLLPPGSIPAQMAQNAANV
                     IAGLTNFNTTLDILTINIPGATTATLSFGAPVQLIFGLLGAPGNALSALNSTGVALAG
                     AVQAGNASAAAAALLDAPAFMANGFLNGTTFVTLPPTALTLLGVIPATSTVTIPLGGL
                     LTPLSFPTAETSALGLTLPVDIVGGTPVGGLIPTMLSIDSQLARAITPIT"
     gene            2044..2211
                     /locus_tag="VFI55_10220"
     CDS             2044..2211
                     /locus_tag="VFI55_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074440.1"
                     /translation="MTISVFAAFVTNSGRCGFAQVKGSGRAPNEPTTAPVRQSDASSG
                     ILRLGCADSHH"
     gene            complement(2333..2854)
                     /locus_tag="VFI55_10225"
     CDS             complement(2333..2854)
                     /locus_tag="VFI55_10225"
                     /inference="COORDINATES: protein motif:HMM:NF013127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HET7074441.1"
                     /translation="MSFVSVTPELVQGAVEDLTGIRSSLAEAAAIATGPTTGIASAAG
                     DEVSIALASMFGNFGREFQVMSAQAQAFHAEFVSLMNAGVDAYLSTEAANVEQTLLGG
                     LSAPASAPAAALEGLNSFGATVAAPYQTLVADTVNNLQSLGSAITANPAPFLQQFAPS
                     RSAESLLRWSSVR"
     gene            complement(3007..5502)
                     /gene="pheT"
                     /locus_tag="VFI55_10230"
     CDS             complement(3007..5502)
                     /gene="pheT"
                     /locus_tag="VFI55_10230"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET7074442.1"
                     /translation="MRVPYSWLREVVWAGAPDWDVAPDDLEQTLVRIGHEIEEVISLD
                     QVDGPLTVGRVTEIEELSGFKKPIRACLVDVGEERRREIVCGATNFVVGDLVVVALPG
                     ATLPGGFAIAARKTYGRASDGMICSTAELGLGADHSGILVLPPGTAAPGADGAGVLGL
                     DDVVFHLAITPDRGYCMSVRGLAREVACAYDLNFVDPASNDVVKPLPAEGEAWPLTVQ
                     AETGVRRFALRPVTGIDPTAVSPWWLQRRLLLSGIRATSPAVDVTNYVMLELGHPMHA
                     HDRNRITGGFGVRFARPGETVVTLDDIERRLDPADVLIVDDVATTAIGGVMGAASTEV
                     RADSTDVLLEAAVWDPAGISRTQRRLHLPSEAARRYERAVDPAISVAALDRCAALLAE
                     IAGGAVSPTLTDWRGDPPREDWSQPPIRITVDLPERFAGVAYAPGATARRLTQIGAAV
                     AENDDVLIVKPPSWRPDLLQPADLVEEVMRLEGLEVIPSVLPPAPAGRGLTAAQKRRR
                     AIGKSLAQSGYVEILPTPFLPAGVFDLWGLPADDPRRTTTHVLNPLEADRPQLATTLL
                     PALLEALGRNVSRGIVDVSLFALAQVVQPTAGTRGVELVPVHRRPTDAEIALLDASLP
                     RQPQHVAAVLTGLREPRGPWGPGRRVEAADAFEAARIVARASGIDVVLGAAQYLPWHP
                     GRCAEVLVGATCIGHAGQLHPAVIERSGLPKGTCAMELNLDAIPLVEALPAPRVSPFP
                     AVFQDVSLVVSAHVSAQAVADAVHEGAGELLEDIQLFDVFTGPQIGENRKSLTFALRF
                     RAPDRTLTEDDASAARDAAVRRAAEAVGAELRA"
     gene            complement(5502..6545)
                     /gene="pheS"
                     /locus_tag="VFI55_10235"
     CDS             complement(5502..6545)
                     /gene="pheS"
                     /locus_tag="VFI55_10235"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET7074443.1"
                     /translation="MGDQPVDLSPAALATAVAAARQAMAVADDLDVLARVKTEHLGDR
                     SPLALARQALGGVPKDERADAGKRVNTARGEAQRSYDERLAALRAERDAAVLVAEGID
                     VTLPSTRQPSGARHPITILAEHIADTFIAMGWELADGPEVETEQFNFDALNFPADHPA
                     RSEQDTFYVAPEGSRQLLRTHTSPVQVRTLLQRELPVYIISIGRTFRTDELDATHTPV
                     FHQVEGLAVDRGLSMAHLRGTLDAFARAEFGPSARTRIRPHFFPFTEPSAEVDVWFAD
                     KKGGADWVEWGGCGMVHPNVLRATGIDPEVYSGFAFGMGLERTLQFRNGIPDMRDMVE
                     GDVRFSLPFGVGA"
     gene            complement(6671..7492)
                     /locus_tag="VFI55_10240"
     CDS             complement(6671..7492)
                     /locus_tag="VFI55_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid-like protein"
                     /protein_id="HET7074444.1"
                     /translation="MIGGILSRLTRLRFTVAYAAVLLAVSCVILALGPHAHDVLVQRA
                     STNLHNLAHGHLGTLLGSALVVDAGPLYFWLPFLTCLLALAELHLRTIRLVVAFLVGH
                     IGATLVVAAALAAAVELGWLPMSITRASDVGMSYGALAVLGAMTAVIPQRFRAPWVGW
                     WVTAGLVAAIVGGDFTDAGHTVAVILGVLVSARFRQPVHWTLVRFLMLVASSGFGFLV
                     LAHHWGTMAAVPVFGALGAFVAYKIGQRGADDSALPVLPGADDDTLTGVQPALTG"
     gene            complement(7510..8340)
                     /locus_tag="VFI55_10245"
     CDS             complement(7510..8340)
                     /locus_tag="VFI55_10245"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7074445.1"
                     /translation="MSIRAVAEWLHTTNHSPGVVAFLRRARRLLPGDPEFGDPLSTAG
                     EGGPRAAARAADRLLGDRDAATREVSLGVLQVWQALSEKISRRPANPEVTLVFTDLVG
                     FSTWALEAGDDAALTLLRQVARAVETPLLDAGGHIVKRMGDGLMAVFREPAVAVGAVL
                     AAKEQLKSVEVAGYTPRMRVGIHTGRPQRMAADWLGVDVNIAARVMERATKGGVMVSS
                     STLDLIPQSELDAFGVVAKRARKPVFAAKTAGVPADLAIYRLRTLKELTASDYTSETN
                     "
     gene            8723..>9719
                     /locus_tag="VFI55_10250"
     CDS             8723..>9719
                     /locus_tag="VFI55_10250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HET7074446.1"
                     /translation="MTATPRTSATAADPLGPDSLTWKYFGDLRTGMMGVWIGAIQNMY
                     PELGAGVEEHSILLREPLQRVVRSVYPIMGVVYDGDRAALTGQQIKRYHRTIKGVDAT
                     GRRYHALHPDTFYWAHATFFMLIIKVAEYFCGGLTEAEKRQLFDEHVQWYRMYGMSMR
                     PVPASWEEFQQYWDRVCREELEINQATLDIFQLRIPKPRFVLMPTPVWDRLFKPMVAG
                     QRWIAAGLFEPAVREKAGMHWTPGDEVLLRVFGKLVELAFLAVPDEIRLHPRALAAYR
                     RAQGRVPGDAPLVEAPKFVAPPRDRRGLPMHYFPRGSRPSLRSALDPALQPARALM"
BASE COUNT         1748 a         3212 c         3209 g         1550 t
ORIGIN      
        1 cgatttttcc atcttcccga attaaaaaca tccaaggatc gcaagggatt tgctcgagct
       61 ctgtagtccc attccataca gcggccgtcg agtcgagaaa gcatttcaga aaccaagatt
      121 cgccggaggc gattgagggc caggacgccg accaaacatc ggcaacctgg tcctcaacga
      181 ctacgtgatg ggtgtgattg cgcgtgccag ctgggagtca atgctcaaca tggttggaat
      241 caggccaccg accggggtgc cgcccacaat atcgaccggc agtgtgaggc caagagcaga
      301 agtctcagct gtggggaaac tcagcggagt gagcagccct ccgaggggga tggtgaccgt
      361 ggaagttgcc ggtataacac ccaggagtgt caaagcagtc ggaggcagcg ttacgaatgt
      421 tgtcccgttg aggaagccat tcgccatgaa tgctggagcg tcgaggagtg cggcggcggc
      481 cgcgctcgcg tttccggctt gcacggcgcc ggccaatgcc actccggtgg aattcagcgc
      541 gctcagtgca ttccccggcg cacccagcaa gccaaaaatc aactgcactg gcgccccaaa
      601 ggacagagtg gccgtcgtag caccaggaat attaatagtg agaatatcaa gcgtggtgtt
      661 gaagtttgtg agccccgcaa tcacattggc cgcattctgc gccatctgcg cggggataga
      721 gccgggaggc agcagtgtga cgaagttctg cgccatctgt cccgggatat ccaaaatggg
      781 ccccaaatct cccaaaggac ccatcggtgt gacaaggaac tcgccaagac ccgatgcccc
      841 tggcgctacc tggatgctat tgaaaccagg gaggaatgca tttactaaag cctgattgag
      901 gtcgccgtat gcgccgacgg ggttgccggc caacaggtcg tggaaggccg tctggacgcc
      961 cgccgaaaac gtgggcagcc cggcgctgaa gtccccggcc gcattctgaa gcgacgtact
     1021 ggtcgtctga ccggtagcga tctggccgtt gacgaactgc tgcagcaaag cccccgggtt
     1081 aaaggtcgaa gcgccctgga tggcaagttg aacgttcgcc ggcacgttcg ccgggaagcc
     1141 ctggaggtca agtgcgaccc cattgaagaa cgtgcgcgcg taaccctgct ggttgatgct
     1201 gagctgattc aggacgggaa aaggattggc ggcgatggtg ctaccgatgg cttgcaggtt
     1261 ggtttgcgta ttcgcggcga gtgcctggta gggggccgcg acaccggccc agaactcgtt
     1321 aatgccctgg atcaccgtgg gcgccgcagc gggtgcgccg gccacgcccg ccgaaatcgc
     1381 ttgcgcgtcg acctgaatct gggccgacac cagcgcgcca aagctgccgt tctgcagcgc
     1441 agtggcggcg ccattcagcc cattgccgat gttgccggcg aactgcccga tagccgtccc
     1501 tccacccgcg aaggcgccgc ccaggttttg gccgacgctg ccgagaaccc cgccgccaag
     1561 caggccctgc ccggcgttgg cggcctcggt gctcaggtag gcacccgcgc cggcgttcaa
     1621 caatccgaca aactgctcat ggaatgcctg ggcctgggcg ctgagagcct gaaactcctg
     1681 gccgaagttg ccgaacatcg atgagatcgc gatcgatacc tcgtcctggg cggcaaccgc
     1741 gatcccggta gtgggacccg ctgcggtcgc ggcggcttcg gccaatgacg aacgaacacc
     1801 cgccagattt tgggccgcgc tctcgaccag atccggcgtt gcgatcacga acgacatatc
     1861 gagctctcct cggttcactg cgatgaccgg tggtcatcgc gttgggcata agtcgggcga
     1921 gggcccgcgc acggatgcgc cgagcccccc tcgactcatt ggtttggccg gttaccggtc
     1981 ggaaacttac ccatcggtaa cccagacaaa cacgagccta ataagccgtt atcatgccag
     2041 aaaatgacaa tcagcgtttt cgcagctttc gttacgaatt cgggcaggtg cggcttcgcg
     2101 caggtcaaag ggtctgggcg ggcgccgaac gagccgacca ccgcgccggt gcgccagagc
     2161 gacgcttcgt cgggcatctt gcggctcggc tgcgccgaca gccaccacta gctgattgga
     2221 ggcgcgccga ttgcctccgc gagctgttcg ggcaggaagg ccagcaggcc cggcaggata
     2281 cctccgaagg tggtgccgct cagcgtggtc gatcctggca ctcccgagat gctcagcgaa
     2341 ccgaactcca gcggagtaag gattccgccg agcgggatgg ggcgaactgc tgcaggaacg
     2401 gcgccggatt cgcggtgatc gcactgccga ggctttgcag gttgttgacc gtatccgcga
     2461 ccagcgtttg atatggggcg gcgacggtgg cgccgaagct gttgagcccc tcgagcgcgg
     2521 ccgctggtgc ggaggcaggc gcgctgagcc cgccgagcaa agtttgctcg acgttggccg
     2581 cctcggtgct caggtaggcg tcaacccccg cgttcatcaa cgacacgaat tcggcgtgaa
     2641 acgcctgcgc ttgcgcgctc atgacctgga attcccggcc gaaattgcca aacatcgaag
     2701 cgagcgcgat cgacacctcg tccccggcgg ccgacgctat cccggtcgtg ggccccgtcg
     2761 cgatggcggc ggcttcggcc agcgacgaac gaatcccggt cagatcttcg actgctccct
     2821 ggactaattc aggcgtcacg ctcacaaacg acatgcccgg ctcctcgact cttcgctcga
     2881 tgcccagttc ttactttcaa gtaagatttt ttcggccaag ccgacgaaga ccatcggtca
     2941 aagggcgtag aaaacgccgt cggagggtcg tagatcgtgc ccgcggggtc gcggtgttgc
     3001 tgggtttcag gcgcgaagtt cggcgccgac cgcctcggcg gcgcgtcgca ccgcagcgtc
     3061 gcgggccgca ctcgcgtcgt cttcggtcaa cgtgcgatcc ggtgccctga aacgcagtgc
     3121 gaatgtcagc gatttgcggt tctcgccgat ctgtgggccg gtgaacacgt cgaacaattg
     3181 aatgtcttcg agcagttctc cggccccctc gtgaacggcg tccgcaacag cctgagcgga
     3241 cacgtgcgcc gacaccacga ggctgacgtc ctggaacact gccggaaacg gcgacaccct
     3301 gggcgccggc aacgcctcga cgagaggaat cgcatccagg ttcagctcca ttgcgcaggt
     3361 gcctttgggc aggcctgagc gctcgatcac ggcgggatgc agctgccccg catgaccgat
     3421 gcaggtcgcc cccaccagca cctcggcgca ccggccggga tgccagggca gatactgggc
     3481 cgcgcccagg accacatcaa ttccactggc gcgcgcgacg attcgtgccg cctcgaacgc
     3541 gtcggcggct tcgactcggc gacccgggcc ccagggacct cgcggttcac gcagaccggt
     3601 cagcaccgca gcgacgtgct gcggttgtcg cggcaaggag gcgtccagca gcgcgatctc
     3661 ggcatcggtg ggccggcggt gaacggggac gagctccacg ccacgagtcc ccgcggttgg
     3721 ctgcacgacc tgcgccaggg cgaacagcga cacatcgacg ataccccggg acacgttgcg
     3781 ccccaacgct tccagcaatg cgggcagcag cgtggtggcc agctgcggac ggtcggcctc
     3841 cagcgggttg aggacatgtg tcgtcgtccg tcgcggatca tcggccggca gaccccacag
     3901 gtcgaacacg ccggcgggca aaaacggcgt cggcaggatc tccacataac cggactgggc
     3961 cagcgactta ccgatggcgc ggcgccgctt ttgcgccgcg gtgaggcccc ggcccgcggg
     4021 agccggtggc agcaccgacg ggatgacctc cagcccctcc agccgcatga cctcctcgac
     4081 caggtcggcc ggctgcaaca gatcgggccg ccaactcggc ggcttgacga tgaggacgtc
     4141 gtcgttctcg gccaccgccg cgccgatttg ggtcagccgc cgggcggtcg cgcccggggc
     4201 gtaggcgacc ccggcgaagc gctccggcag gtcgacggtg atccggatcg gcggctggga
     4261 ccagtcctcc cgcggcgggt cacctcgcca atcggtcagg gtcggtgaca ccgctccccc
     4321 ggcgatctcg gcgagcaacg cggcacaccg gtcgagcgcg gcgaccgaaa tggccgggtc
     4381 caccgcacgc tcataacgac gggccgcctc gctgggcagg tgcaacctgc gctgggtgcg
     4441 cgatatgccg gccgggtccc acaccgcggc ctccagcagc acgtcggtgg aatcagcccg
     4501 cacctcggtg cttgccgcgc ccatcacgcc gccgattgcc gtggtcgcca cgtcgtcgac
     4561 gatgaggaca tcggccggat cgagccggcg ctcaatgtcg tccagcgtga cgacggtttc
     4621 gccgggccgc gcgaaccgca cgccgaagcc gccggtaatg cggttgcgat cgtgggcgtg
     4681 catgggatgg cccagctcca gcatcacgta gttggtgaca tccaccgccg gggaggtcgc
     4741 gcgaatgccg gacagcagca gccggcgctg cagccaccac ggcgacaccg ccgtcgggtc
     4801 gatcccggtg acgggccgca gagcgaaacg gcgcacgccc gtttcggcct gcaccgtcag
     4861 cggccacgcc tctccttcgg ccggcagcgg ctttacgaca tcgttgctgg cggggtcgac
     4921 gaagttgagg tcgtaggcgc aggcgacctc acgggccagg ccgcgcaccg acatgcagta
     4981 accacggtcg ggcgtgatgg caaggtggaa gaccacgtcg tcgagcccga gcaccccggc
     5041 accatctgcg ccaggtgcgg cggtgccggg cggcagcacc aggatcccgg aatggtctgc
     5101 gcccaaaccg agttcggcgg tcgagcaaat cattccgtcg gaggcacggc cgtacgtctt
     5161 gcgggcggcg atggcaaacc cgccgggcaa cgtggcgccc ggcagtgcca ccacaaccag
     5221 atctccgacc acgaagttag ttgcgccgca gacaatctcg cgtcgccgtt cctccccgac
     5281 gtccaccagg caggctcgga tcggcttctt gaaaccgctg agctcctcga tttcggtgac
     5341 ccgccccacg gtcagcgggc cgtcgacctg gtcgaggctg atcacctctt cgatctcgtg
     5401 gccgatgcgc accagcgtct gctcgagatc atcgggggcg acatcccagt ccggggcgcc
     5461 ggcccacacg acctcgcgca gccagctata ggggacacgc atcaggcacc caccccgaac
     5521 ggcaacgaga accggacgtc gccctcaacc atgtcgcgca tgtccgggat cccgttgcgg
     5581 aactgcaggg tgcgttccag acccatcccg aatgcgaagc cggaatagac ttccggatca
     5641 atcccggtgg cccgcaacac gtttggatgc accattccgc atccgcccca ctccacccag
     5701 tcggctccgc cctttttgtc ggcgaaccag acatcgacct cggcggaggg ctcggtgaag
     5761 gggaagaagt gtgggcggat ccgggtgcgt gcggaaggac cgaactcggc gcgcgcgaac
     5821 gcatccagcg ttccgcgcag atgcgccatg gacagaccgc ggtccaccgc caagccttcg
     5881 acctgatgga agacgggagt atgggtggcg tcgagctcgt cggtgcggaa cgtgcggccg
     5941 atcgagatga tgtagacggg cagctcgcgc tgcagcagtg tgcgcacctg caccggcgag
     6001 gtgtgcgtgc gcagcagctg ccgggaaccc tccggcgcga catagaaggt gtcctgctcg
     6061 ctgcgggcgg ggtggtcggc ggggaagttc agggcatcga agttgaattg ctcggtttcg
     6121 acctctggcc cgtcggccag ttcccatccc atcgcgatga aggtatcggc gatgtgctcg
     6181 gccaatatcg tgatcgggtg ccgggcgccg gacggctgcc gagtcgacgg caaggtgacg
     6241 tcgatgcctt cggcgaccag caccgccgcg tcgcgttcgg cgcgcagcgc cgccagccgc
     6301 tcgtcatagc tgcgttgggc ctcgccgcgc gcggtgttga cgcgtttgcc ggcgtcggcg
     6361 cgttcgtcct tgggcacgcc gcccagggct tggcgcgcga gcgccagcgg cgagcggtcg
     6421 ccgaggtgct cggtcttgac gcgtgccagc acgtccagat cgtcggcaac tgccatggcc
     6481 tgccgggcgg cagcgaccgc cgtggccaag gctgccggcg acaggtcgac gggttgatcg
     6541 cccacgcggc gacactctcc ttgctgacga ggctgttcgg gctggcgcca cgagcgtgac
     6601 gcaactgccg atcataggtg attcggtgca cggcgctcgg cgcgtgcggc gaagacggcc
     6661 gacgcgctga ctagccggtc aaggccggct gcacgccggt cagcgtgtcg tcgtccgccc
     6721 cggggagaac cggcagcgca ctgtcatctg caccgcgttg gccgattttg taggccacaa
     6781 aggcgcccag ggcgccgaac accggcacgg ccgccatcgt cccccagtgg tgggccagca
     6841 ccaggaaccc aaaccccgag gacgccacca acatcagaaa ccgcaccaga gtccaatgga
     6901 ccggctgccg gaatcgggcg gacaccagca cgcccaggat caccgcgacg gtatggcccg
     6961 catcggtgaa atcgccaccg acgatcgcgg cgaccagacc cgcggtaacc caccagccga
     7021 cccagggcgc ccgaaagcgc tggggaatca ccgccgtcat agcgccgagc accgcgaggg
     7081 cgccataact catcccgaca tcgctggcgc gagtgatcga catcggcagc cagccgagct
     7141 cgacggccgc ggcaagcgcg gcggccacca ccagggtggc accgatgtgg ccgacgagga
     7201 acgccacgac caggcggatg gtgcgcaaat gcagttctgc gagtgcgagc aggcaggtca
     7261 ggaagggcaa ccagaagtag agcgggccgg cgtcaacgac cagggcgctg cccagcagcg
     7321 ttcccaggtg gccgtgggcc agattgtgca gattggtgct ggcgcgctga accagcacgt
     7381 cgtgcgcatg cgggccgagc gccaggatga cgcagctgac ggcaagcagc accgcagcat
     7441 aagccacggt gaacctgagc cgggtgagcc gggagagtat gccgccaatc attgaatcaa
     7501 ttatatgggc taatttgttt cggaagtgta atcagacgct gtcaactcct taagagttcg
     7561 tagccgatat atcgccaagt ccgcgggaac gccggcggtt ttcgccgcaa acaccggttt
     7621 acgtgcacgt ttagcgacga cgccgaatgc gtccaattcg ctttgcggaa tcagatccag
     7681 cgtcgaactc gacaccatga cgccgccttt ggtcgcgcgt tccatcacgc gggcggcgat
     7741 gttgacgtcg acaccgagcc agtccgcggc cattcgttgc ggtcgaccgg tgtggatccc
     7801 gactcgcatc cgcggtgtgt agcctgccac ctcgacggat ttcagttgct ccttggcggc
     7861 cagcaccgcc cccacggcga ccgccggctc gcggaacacc gccatgagtc cgtcgcccat
     7921 acgtttgacg atgtgcccac ccgcgtcgag caacggcgtc tcgacagccc gcgccacctg
     7981 tcgcagcagg gtgagcgccg cgtcgtcacc cgcttccaac gcccacgtcg aaaagccgac
     8041 cagatcggtg aataccagcg tcacttccgg atttgccggc ctgcgggaaa tcttctcgct
     8101 cagcgcctgc cacacctgca gcacgccgag actgacctcg cgcgttgccg catcgcgatc
     8161 ccccagcagc cggtccgccg cgcgcgccgc ggcgcgtggg ccgccctcac ccgcggtgga
     8221 cagcgggtca ccgaactcgg ggtctcccgg caacagccgt cgcgcacgac gtagaaacgc
     8281 caccacccct gggctgtgat tggtggtgtg caaccactcc gccacggcgc gaatggacac
     8341 cggggcatgg cgatcagggg cgggaggcgc cgtagtcacc gccaccgggt ccgattcggg
     8401 gtccgtttca gggtccgtcg caggagcccc ggcaaagtcg gcttgaacgt cggttgcaag
     8461 gtcctctgga ggggcctcgc ccgggtccgg atcgcgaggc gcgggttcca cgtcgccagg
     8521 ttaggtggtt attgcagaag cgggcaatga cagtcgcccc gaatgtgtcc cgtctcgcat
     8581 caaacggcct gatcacaggc cagatcacga ccccgccaca ccgccgtgga aactcgataa
     8641 caccctcccg cgcaccctcc gaaattgtag acaacgtgcg ttgtcaaagt tatcgtgagc
     8701 gcagttaggg caggaggccg agatgactgc tactccaagg acatccgcga cagccgccga
     8761 tccgctcgga cccgattcgc tcacctggaa gtatttcggt gacctgcgca ccggaatgat
     8821 gggcgtctgg atcggcgcca tccagaacat gtatccggaa ctgggcgcgg gagtcgaaga
     8881 gcactccatc ctgttgcgcg agccgttgca gcgggtggtc cggtcggtgt atccgatcat
     8941 gggcgtggtg tacgacggtg accgggcggc gctgaccggc cagcagatca agcgctacca
     9001 ccgcaccatc aagggcgtcg acgcgacggg ccgtcgctat cacgcgctgc acccggacac
     9061 cttctactgg gcgcacgcca cgttcttcat gctgatcatc aaggtggccg agtacttctg
     9121 tggtggcttg accgaggccg agaagcggca gcttttcgac gagcatgtgc agtggtaccg
     9181 gatgtacggg atgagcatgc ggccggtgcc cgcatcctgg gaggagtttc agcagtactg
     9241 ggaccgggtc tgccgcgagg agctggaaat caaccaggcg acgctggata tcttccagct
     9301 gcggattccc aaaccgaggt tcgtgctgat gccgacgccc gtgtgggacc gattgttcaa
     9361 gccgatggtg gccggccagc gctggatcgc ggcgggacta ttcgagcctg cggttcggga
     9421 gaaggcggga atgcactgga cgccgggcga cgaggtgctg ctgcgcgtgt tcggcaagct
     9481 cgtcgaactg gctttcctgg cggttcccga cgaaatccga ctccaccccc gtgcgctggc
     9541 cgcctaccgc cgcgcccaag gacgggttcc cggcgacgcg ccgttggtcg aggcaccgaa
     9601 gttcgtggcg ccgccgcgtg atcgccgcgg gctgccgatg cactactttc cccggggctc
     9661 ccgcccctcg ctgcgctccg cgcttgaccc cgccctccaa cctgctcgag cgctgatgg
//
LOCUS       DASSGQ010000235         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_351396,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000235 DASSGQ010000000
VERSION     DASSGQ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351396"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..1192
                     /locus_tag="VFI55_10255"
     CDS             224..1192
                     /locus_tag="VFI55_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET7074447.1"
                     /translation="MVIKRKREAGRCADDATESTRTVLIALGANVGVAVAKTVAAVLS
                     GSASMIAEAAHSWADTGNEVFLLIADRRSKRPPDAQHPLGYGREAYMWSLLAAVGLFA
                     AGAMVSVWHGVADMIQGGSSLGDARVAYLVLVIALVMESTSLRQAVHQLRGEAEVYHR
                     DVLEHALETSDPTLRGVFAEDSAAVIGIALAFAGIGLHQLTGNAVWDSLASILIGVLL
                     GVIAVILIDRNRRFIAGEAGSDELRDVVVARIEALPDVAAVRFVRLIYVGPRRLFLVA
                     SVDLAGDAPESHVAKKLRRLEHQLESDPHIVDAVLTIADPDDADRI"
     gene            complement(1208..1717)
                     /locus_tag="VFI55_10260"
     CDS             complement(1208..1717)
                     /locus_tag="VFI55_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HET7074448.1"
                     /translation="MVHSIELVFDPGTEAAIRNIWADLATAGIPSQAPASRPHVTLVV
                     AERIAPDVDELLGPVSERLPLAAAVGAAVLFGRANVVFARLVVPTSEMLALHAEVHRL
                     CCPHVEPKPMPNSLPGQWTPHVTLARRVGGTRLGRALRIAGRPSQIDGQFAGLRRWDG
                     NKRVEHPIG"
     gene            complement(1717..>2345)
                     /gene="bioD"
                     /locus_tag="VFI55_10265"
     CDS             complement(1717..>2345)
                     /gene="bioD"
                     /locus_tag="VFI55_10265"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HET7074449.1"
                     /translation="TAALACHARQCGIDVAVCKPVQTGTDAGDDDLAEVARLSGVTEL
                     AALARYPQPLAPAAAAEQAGRALPTGDQLVQLIAGVDRPGRLTLVEGAGGLLVELADA
                     GITLRDLAVELGAAALIVATAEMGTLNHTALTVEALAARGVSCAGLVIGSWPPHPGLL
                     ETSNRSALARLAPVRAALPAGAGSRDAAGFADLSARAFDRDWVTGLVG"
BASE COUNT          364 a          792 c          824 g          365 t
ORIGIN      
        1 gcatctttgt catctttgtc cagggccgat gcgaaacgca tcagcccacc tcggtgccac
       61 gtggcatggc agccagaacg aaacggtgtt ccaagaaggg taacggccgc agaacgtggg
      121 gaccgccggg gtatgtcacc agcgactcgc aagcgactca ccaacacacg atcgccccga
      181 ccacacgccg cgccgcgaaa tctgcgggat gatcggcgct gcgatggtta tcaaacgaaa
      241 gcgcgaagct ggccgctgcg ccgacgacgc caccgaaagc acccgcacgg tgctgatcgc
      301 gctgggcgcc aacgtcggcg tagcggtcgc gaagaccgtg gctgcggtgc tctccggctc
      361 cgcgtcgatg atcgccgagg ccgcgcattc ctgggccgac accggaaacg aggtgttctt
      421 gctgatcgcc gaccggcgaa gcaagcgacc ccccgatgcc caacatccac tgggctatgg
      481 ccgcgaggct tacatgtggt cattgctggc ggcggtgggt ctgttcgcgg cgggtgccat
      541 ggtgtcggtg tggcacgggg tggccgacat gattcaaggc ggctcgagcc ttggtgacgc
      601 tcgcgtcgcg tacctggtgc tggtcatcgc gttggtgatg gagtcgactt cgctgcgaca
      661 agcggtccac cagctgcgcg gtgaggccga ggtttatcac cgcgatgtgc tggagcatgc
      721 actcgaaacg tcggatccca cgctgcgcgg cgtgttcgcc gaggacagtg ccgcggtgat
      781 cgggattgct ttggcctttg ccggcatcgg gctgcaccag ctcaccggaa atgcggtgtg
      841 ggactccctg gcatcgatct tgatcggcgt cttgctaggg gtgatcgcgg tgatactgat
      901 cgaccgcaac cgacgcttca ttgccggcga agcgggttca gatgagctgc gtgacgtggt
      961 ggtggcccgg atcgaggcgt tacccgacgt cgccgcggtg cgcttcgtgc ggctgatcta
     1021 cgtgggaccg aggcggctgt ttttggtggc cagcgtcgac ctggcgggcg acgccccgga
     1081 atcgcatgtg gcgaagaagc ttcgccgcct ggaacatcag ctggaatccg atccgcatat
     1141 cgtcgatgcg gtcctgacaa tcgccgatcc cgacgacgcc gaccgcatct gagcctgccg
     1201 cggcgcatca gccgatcggg tgctcgaccc gcttgttgcc gtcccagcgg cgcagacccg
     1261 cgaactggcc gtcgatctgc gacggccgac cggctatgcg cagcgcccgc ccgagtcggg
     1321 tgccgccgac ccggcgggcc agcgtgacgt gtggcgtcca ttgaccgggc aggctgttgg
     1381 gcatcggctt tggctccaca tgcggacagc agagccgatg cacctcggca tgcagggcca
     1441 acatctcgct cgtcgggacg acgagccgtg cgaacacgac gttcgcccgc ccgaacaaca
     1501 ccgccgcacc gacggccgcg gccaagggga gccgttcgct gaccggaccg agcagctcgt
     1561 cgacgtcggg ggcgatgcgt tcggcaacca ccagcgtcac gtgcggccgg ctcgccggcg
     1621 cctggctggg gattccggcg gtggccaggt ctgcccagat gttgcggatc gccgcctcgg
     1681 tgccgggatc gaagacgagc tcgatcgaat ggaccatcag ccgaccagcc cggtgaccca
     1741 gtcgcggtcg aacgcccggg cgctcaggtc cgcgaaaccg gcggcgtccc tcgatccggc
     1801 gcccgcgggc agcgcggccc gcaccggggc caggcgggcc agcgccgacc ggttcgaggt
     1861 ctccagcaac ccggggtgtg gcggccagct cccgatcacc aacccggcac acgaaacgcc
     1921 tcgcgccgca agggcttcca cggtgagcgc ggtgtggttg agggtgccca tttcggcggt
     1981 ggcgacgatc agcgccgcgg cacccagctc gacggcgagg tcacgcagtg tgatgcccgc
     2041 gtctgcgagc tcgaccagca gcccgccggc gccttcgacc agggtcagcc gccccgggcg
     2101 gtccacgccg gcgatcagct gcaccagctg gtcaccggtg ggcagcgccc gcccggcctg
     2161 ctcggccgcg gcggccggtg ccagtggctg cggatatctg gccagcgcgg cgagctcggt
     2221 caccccggac agtcgggcca cctcggcgag gtcgtcatct ccggcgtcgg tgccggtctg
     2281 gacgggttta cacaccgcca cgtcgatgcc gcactgacgg gcgtggcacg ccagcgccgc
     2341 ggtga
//
LOCUS       DASSGQ010000236         2635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_987703,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000236 DASSGQ010000000
VERSION     DASSGQ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987703"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..2338
                     /locus_tag="VFI55_10270"
     CDS             257..2338
                     /locus_tag="VFI55_10270"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET7074450.1"
                     /translation="MDDHKRTTRRLDGGRRLLSNPRSAREALRAGFHSLPSVTVAHLL
                     RRIPPVSSTPNAEPSPSGQHDETGGSHLALGGGAQFAGYTILRQLGAGGMAEVYLALH
                     PRLPRRDVIKVLAAAITADREFRDRFNREADLASTLWHPHVVGVHDRGEFNGQLWISM
                     DYVEGTDAARLVKERYQDGMPIDEVCAILHAVAGALDYAHDRGLLHRDVKPANILLTH
                     PEDGERRILLADFGVARHLGDISGITETNVAVGTVAYAAPEQLTGSNIDGRADQYALA
                     ATAFHLLTGAPPFMHANPIAVISQHLHDDPPRLSDYRPDLAHLDDVFFKALAKQPEDR
                     FERCRVFASAVAAVDEEFDEASDVSRGAQPAVMTPPRRRGMRGAVAVLNHRFSSRTRW
                     ATALVCAVLIAVAATWSTLYSFQPSPPTNVPTLASKPSVPAPSAAAARPGGPALNGTY
                     QLTYDRSKQTGNGIAIRSDGANTTWWAFRSVCTTNGCAATGTQLDDANHQVASTADGG
                     HTGTMRFVSGYWQGAPQQERVGCTQPNGQVRATQQETIAWSLAPQADGTLRGTQTETV
                     LSNECGAQGAVVRVPVVATRVGDAPAGVNLADPNQVISSSTAQAHPVPAVLGGMCTDV
                     DKLGYDPTNNEQVVCEGNTWAKAPITMGVHASGSSCDRPGTSVFAMSTSNDGYLLQCD
                     PVTRTWTRPGA"
BASE COUNT          459 a          922 c          853 g          401 t
ORIGIN      
        1 ctttatttcg atttggcctc tcatagccgt ttcatagcct gctgagacca atcgcccacc
       61 gggatccggc atgattcgaa cgtgaaggag ctgatcgaac ggcgcgccga gcacatcacc
      121 gtgccggccc tagataccga catgaagcga acccgctcat gggccccaat cccgatgcaa
      181 acgcccaggg cgtttcgccc cgcgtcaggt tgcggcgatg cccgcaggta ctgccgggaa
      241 aggggggcaa cgacagatgg acgaccacaa gcgaactacc cggcgcctcg atggcgggcg
      301 ccggctgctg agcaacccgc gttcggcccg tgaggccctg cgcgcgggct ttcactcgct
      361 accctcggtg accgtcgcac acctgttacg ccgcattcca cccgtcagct cgaccccgaa
      421 cgcggagccg tcaccttcag gacagcacga cgagaccggc ggatcgcatc tcgcgctggg
      481 cggcggtgcg caatttgccg gctacaccat cctgcggcag ctcggcgccg gtggcatggc
      541 cgaggtctat ttggcattgc accccaggct gccgcgccgc gacgtgatca aggtcctcgc
      601 agcggcgatc acggcggacc gagagttccg cgacaggttc aaccgggagg ccgacctggc
      661 ctcgacgctg tggcacccgc acgtcgtcgg ggtccacgac cgcggcgaat tcaacggtca
      721 gctctggatc tccatggact acgtcgaggg caccgacgcc gcccggctgg tcaaagagcg
      781 ctaccaggac ggcatgccga ttgacgaggt atgtgccatc ctgcacgccg tcgcgggcgc
      841 gctcgactac gcccacgatc gcggcctgct gcaccgcgac gtcaaacccg ccaacatcct
      901 gctcactcat ccggaggacg gggagcgtcg aatcctgttg gcggacttcg gtgtagcgcg
      961 tcacctcggc gacatcagcg gtatcaccga gaccaatgtc gcggtgggaa cggtggccta
     1021 cgcggcgcct gagcagctga cgggttccaa catcgacggg cgggccgatc aatacgcctt
     1081 ggccgcgacg gcgtttcatc tgctcaccgg cgcgcccccg tttatgcacg ccaacccgat
     1141 cgcggtgatc agccagcacc tgcacgacga tccgccgcgg ctgagcgact atcgcccgga
     1201 tctggcgcat ctcgacgacg tattcttcaa ggccctcgcc aaacaacccg aggacaggtt
     1261 cgagcgttgc cgcgtttttg cgtctgctgt tgcggccgtc gatgaggagt tcgacgaggc
     1321 ctccgacgtt tcgcggggag cacagcccgc cgtgatgacg ccgccgcggc ggcgcggaat
     1381 gcgcggcgcg gtggccgtgc tgaaccaccg gttctcgtct cgaacccggt gggcgacggc
     1441 gctggtgtgc gcggtgctga tcgccgtggc cgcgacctgg tccacgctgt actcgttcca
     1501 accgagtcca ccgaccaacg tccccacgtt ggcgtccaaa ccatccgtcc ccgcgcccag
     1561 cgccgcggcg gcgagacccg ggggaccggc gctcaacggc acctaccagt tgacgtacga
     1621 ccgcagcaag cagaccggca acggcatcgc gattcgcagt gatggcgcca acaccacctg
     1681 gtgggcattc cgatcggtgt gcaccaccaa cgggtgcgcg gccaccggga cccagctgga
     1741 cgacgccaac caccaagtgg ccagcaccgc cgatggaggc cacacgggca cgatgcgctt
     1801 cgtcagtggt tactggcagg gcgccccaca gcaggagcgg gtgggctgca cgcaaccgaa
     1861 cgggcaagtc agggccaccc agcaggaaac catcgcgtgg tcgttggcgc cgcaggccga
     1921 cggcaccctg cgcggcacgc agaccgagac cgtgctcagc aacgaatgcg gcgcccaagg
     1981 ggccgtggtg cgggtgccgg tggtggccac ccgagtcggt gacgcgcccg cgggcgtgaa
     2041 tttggccgat cccaaccagg tgatcagcag ttcgaccgcg caggcccacc cggtaccagc
     2101 cgtcctcggc ggcatgtgca ccgacgtgga caagctcggc tacgacccga ccaacaacga
     2161 gcaggtcgtc tgcgaaggca acacctgggc caaagccccc atcacgatgg gagtgcacgc
     2221 ctcgggcagc tcgtgcgacc ggcctggcac gtcggtgttc gccatgtcga cctccaacga
     2281 cggctacctg ctccaatgcg atccggtcac ccgaacctgg acccgtcccg gcgcatagcg
     2341 ggaccgccgg ctgccgcgcg caccgattga cgactttcga aagtgctatc acatgcgcta
     2401 gcaccttcgc ggatcaagtg gtggtgctcc cgcggtactg atgtggcgga tgtgactgcc
     2461 ggcgtcttga gcgccgaact cgcgtctgcg ggaggcagcg tgtcgggggc tggttctacc
     2521 ctggtggcat ggctttcgcc accgaacacc cagtagtcgc gcactccgag taccgcgccg
     2581 tcgatgacgt cgtgcgcgcc ggcggcgaat tcgaggtcgt cagcccgcac gagcc
//
LOCUS       DASSGQ010000237        10663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_775591,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000237 DASSGQ010000000
VERSION     DASSGQ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10663
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775591"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFI55_10275"
     CDS             complement(<1..678)
                     /locus_tag="VFI55_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HET7074451.1"
                     /translation="MRARSYCVVGGGISGLTAAYRLRAGVGDDATITLFDPGDRLGGI
                     LRTELVGGRPMDLGAEAFVLRRPEMPALLAELGLSGRQLATTGARPLIYSEQELHPLP
                     PGTVVGIPSSAAAVAGLVDEATVARIAAEPAHPVPWRPGDDPAVAELVGDRFGEQVVA
                     RSVDPLLSGVYAGSAATIGLRAAAPSVAAALDRGATSLTDAVRQGLPPATGAPVFGAL
                     DGGYRVLI"
     gene            complement(675..1769)
                     /gene="hemE"
                     /locus_tag="VFI55_10280"
     CDS             complement(675..1769)
                     /gene="hemE"
                     /locus_tag="VFI55_10280"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020728429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HET7074452.1"
                     /translation="MSNPDIPPRARRELPESPYLAALTGRKPTQVPVWFMRQAGRSLP
                     EYRALRERRSMLAACFEPEMACEITLQPIRRYGVDAAILFSDIVVPLRAAGVDLDIVA
                     DVGPVIAHPVRTEADIDAMKPLDPQAIQPVKDAVSLLVGELGDVPLIGFAGAPFTLAS
                     YLVEGGPSRNHARTKAMMLAETASWHALMAKLTDLTVAFLRGQIEAGVDAIQVFDSWA
                     GTLSLADYRHYVLPHSSRVFAALAEYGVPMTHFGVGTAELLGAMSAALKAGEQPAKGT
                     AVGVDWRTCLADAAARVEPGTALQGNLDPVVLLAGWPVVERAARAVVDDGRRAVDAGA
                     AGHVFNLGHGVLPETDPGVLTELVSLVHSL"
     gene            1923..2741
                     /locus_tag="VFI55_10285"
     CDS             1923..2741
                     /locus_tag="VFI55_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET7074453.1"
                     /translation="MPISYEGFPGLRCEPGDETGLDGVLTLVLDAPGLNAVGPQMHRD
                     LADIWPVIDRDPAVRAVLVRGEGKAFSSGGSFELIAETIGDYEGRLRIMREARDLVLN
                     MINFDKPVVSAIRGPAVGAGLVVGLLADISVAGRTAKLIDGHTKLGVAAGDHAAICWP
                     LLVGMAKAKYYLLTCETLLGEEAERIGLVSTCVDDDDVLPTATRLAENLAQGAQNAIR
                     WTKHSLNHWYRMFAPTFETSLGLEFLGFSGPDVQEGLAAHREKRPARFTGGNTT"
     gene            2861..3079
                     /locus_tag="VFI55_10290"
     CDS             2861..3079
                     /locus_tag="VFI55_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074454.1"
                     /translation="MTCAATMQSDCDAEERRQTASAATMQSDSDAEERRQTASAATMQ
                     SDSDAEDRRRKACAATTQNGAMRRSRRQ"
     gene            3076..3657
                     /locus_tag="VFI55_10295"
     CDS             3076..3657
                     /locus_tag="VFI55_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3000 domain-containing protein"
                     /protein_id="HET7074455.1"
                     /translation="MTSAEPAPFSEAVAAMNAVTVRPEIELGPIRPPQRLAPYSYALG
                     AEVKHPDLEIVPERSEGDAFGRLILLYDPDGADAWDGTLRLVAYIQADLDPSEAVDPL
                     LPEVAWSWLVEALQSRMELVTALGGTVTATTSVRYGDISGPPRAHQLELRASWTATTP
                     QVGVHVEAFCEVLEHAAGLPPAGVTDLGSRSRA"
     gene            3659..4960
                     /locus_tag="VFI55_10300"
     CDS             3659..4960
                     /locus_tag="VFI55_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HET7074456.1"
                     /translation="MCEPAAKVPDRTAPESAESSPTPLRHPADGMPDLSVTVREIEAA
                     AQLLDGGRGPFAVDAERASGFRYSNRAYLIQIRRAHAGTVLIDPVSHGADPLTALRPV
                     ADVLSTDEWILHSADQDLPCLAEVGMRPPALYDTELAGRLAGFDRVNLATMVERMLGF
                     GLTKGHGAADWSKRPLPADWLNYAALDVELLIELRAAISDVLAEQGKTDWAAQEFDYL
                     RTFKAGDAAGEVAPAARRERWRRTSGIHRVRDPRALASVRELWTTRDHIAQRRDIAPR
                     RILPDSAIIDAALADPKTVEALVELPVFGGRNQRRSAEMWLAALEAARQNQHPPDDAE
                     PPNGPPPPARWSRRKPEAAARLEAARAALAEVSERVGVPTANLVSPALVRRLCWDWAG
                     APDPVEAVEDFLCAGHARAWQRELVDPVLARALQPPADTED"
     gene            complement(4992..6917)
                     /gene="dxs"
                     /locus_tag="VFI55_10305"
     CDS             complement(4992..6917)
                     /gene="dxs"
                     /locus_tag="VFI55_10305"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HET7074457.1"
                     /translation="MLERIRGPADLQHLSQQQLRDLAAEIREFLIHKVAATGGHLGPN
                     LGVVELTLALHRVFYSPHDPIIFDTGHQAYVHKILTGRSLDFEGLRKKGGLSGYPSRA
                     ESEHDWVESSHASAALSYADGLAKAFELTGHRNRHVVAVVGDGALTGGMCWEALNNIA
                     ASSRPVVIVVNDNGRSYAPTIGGVADHLATLRLQPGYEQLLERGRDALRSMPVIGEVA
                     YHFVHSVKAGIKDSLSPQLLFTDLGLKYVGPVDGHDEHAVEAALRHARGFGRPVIVHV
                     VTRKGMGYRPAEDDEAEQMHSCGVIDPATGQATKIAGPGWTATFSDALIGYAKKRRDI
                     VAITAAMPGPTGLTAFGQRFPDRLFDVGIAEQHAVTSAAGLAMGGMHPVVAIYSTFLN
                     RAFDQIMMDVALHKLPVTMVLDRAGVTGSDGASHNGMWDLSMLNIVPGMRVAAPRDAT
                     RLREELGEALDVDDGPTAIRFPKGDVGEDIPALERRGSGVSGIDVLALPAAGVNHDVL
                     LVAVGAFVPMALAAAKRLHNQGIGVTVIDPRWVLPVSEGVLELAAQHKLVVTCEDNGV
                     NGGVGSAVSAALRSAEIDTPCRDVGLAQRFYAHASRDEVLADLGLTDQDMARRVTGWV
                     AALGSSDSEAEIREHLE"
     gene            6985..7428
                     /locus_tag="VFI55_10310"
     CDS             6985..7428
                     /locus_tag="VFI55_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7074458.1"
                     /translation="MRAAQLAEDFPVVSVDSDALDAARLLAEHRLPGIVVTDRSGEPF
                     AVLPASQVVRFIVPRYVQDDPSLAGVLNESMADRAADKLGGKTVGDMLPDHLTNVPWA
                     HADDTIIEVAAVMARLRSPLIAVVKDKALVGVITASRLLAAALKH"
     gene            7462..8748
                     /locus_tag="VFI55_10315"
     CDS             7462..8748
                     /locus_tag="VFI55_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HET7074459.1"
                     /translation="MSVVAVVVFVVAYALIASDRVNKTLVALTGAAIMVALPVIDSDD
                     VFYSRATGIDWDVLFLLLGMMIIVSVLRQTGVFEYTAIWAAKRAHGSPLRIMILLVVV
                     TAFASALLDNVTTVLLIAPVTLLVCDRLAIGPAPFLMAEAFASNIGGTATLIGDPPSI
                     IIASRAGLSFNDFLIHLTPIVIVITIVFCVLLPRLFPGAFAVDPERVADVMSLEEREA
                     IRDPRLLIKCGVVLMAVLAGFVAHAPLHLAPSIVALLGAGILIVSSRLEPSEYLSSVE
                     WDTLLFFAGLFVMVGALVKTGVVNQLARLATAATGGHVLLTTMVILAASFVFSGLINN
                     VPYAATMAPIVAQLIPSMGHANTQAMWWALVLGTDLGGNLTAVGASANVVILGLARRA
                     DSPISFWEFTRKGAVITTISAALSALYLWLRYFALS"
     gene            complement(8745..>10663)
                     /locus_tag="VFI55_10320"
     CDS             complement(8745..>10663)
                     /locus_tag="VFI55_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET7074460.1"
                     /translation="ISKDGKTGLIVAGITGGEDGAQQHAKQLADQLVYDRDGVTVQAG
                     GDAMTRVQVNSQSEKDLLLMESIAMPLSFVVLVWVFGGVMAAALPLAVGGFAILTSMA
                     VLRAITYATDVSIYALNLTVAMSLALAIDYTLLIVSRFRDELAEGADRDQALVRTMTT
                     AGRTVLFSAMTVGLSMMALMLFPMYFLKSFGYAGIAVVASAAVAAVVVTPAAIVLLGS
                     RLDSVDVRRLGRRLLRRAEPARRPVEQTFFYRSTKFVMRHAIAIGLAIIALLLILGAP
                     FLGIKWGFPDDRVLPDSASARQVGDQVRGAFAVDSATNVTVVIPDLTTVTPRELNSYA
                     VQLSRVPDVSSVSAPGGTFVAGSLVGPPSAAAIKDGSAFFTIGSTAPLFSRASEIQLD
                     RLHALTGPGGQHVQITGIAQISRDSCDAITSRLPAVLAVIAAITFVLLFLLTGSVVLP
                     LKALLLNVLSLSATFGALVWIFQDGHLGAFGTTATGTLVATIPVLLFCIAFGLSMDYE
                     VFLVSRIREYWLMSGSPNADNDETVALGLARTGRVVTAAALVMSISFAALIAAQVALM
                     RMFGVGLTLAVLVDATLVRILLVPAFMRVLGRANWWAPKPLARLHARFGIGEACTPEP
                     IEASSESSGTRVTG"
BASE COUNT         1770 a         3755 c         3544 g         1594 t
ORIGIN      
        1 gatcagcacc cggtagccgc cgtccagtgc gccaaaaacc ggcgcgcccg tggccggcgg
       61 caacccctgc cgcaccgcgt cggtcaggct ggtggcgccg cgatcaaggg ccgccgccac
      121 gctgggggcg gcggcccgca ggccgatcgt cgccgcggag cccgcataca ccccgctcag
      181 cagcggatcc accgatcggg ccacgacctg ctcgccgaac cggtcgccga ccaattcggc
      241 cacggcggga tcgtcgcccg gccgccatgg caccgggtgg gcgggctcgg cggcgatgcg
      301 tgcgaccgtc gcctcgtcga ccaatcccgc caccgcggcg gccgacgaag gaatcccgac
      361 cacggtgccc ggtggcagtg ggtgcaattc ttgctcgctg tagatcagcg gccgggcgcc
      421 cgtggtggcc agttggcgcc ccgacaggcc cagctcggcg agcagcgccg gcatctcggg
      481 ccggcgcagc acgaatgcct cggcacccag atccatcggc cgcccgccga ccagctcggt
      541 gcgcaatatg cccccgagcc ggtcgcccgg atcgaacagc gtgatggtcg cgtcgtcacc
      601 gacccccgcg cgcagccggt aggccgccgt caggcccgag atgccgccgc ccacaacgca
      661 atacgagcgg gctctcatag cgagtggacc agcgacacca gctcggtcag cacgccggga
      721 tcggtctcgg gcaacactcc gtggccgagg ttgaagacgt gacccgccgc gcccgcgtcg
      781 accgcgcggc gtccgtcgtc gaccacggcc cgggcggcac gttccaccac gggccagccc
      841 gcgagcagca ccaccggatc gaggttgccc tgcagcgccg tcccgggttc cactcgggcg
      901 gcggcatccg ccaggcaggt tcgccagtcc accccgaccg cggtaccttt cgccggctgc
      961 tcgccggctt tcagggcagc cgacatcgca cccagtaact cggccgttcc gaccccgaag
     1021 tgcgtcatcg gcacgccgta ttccgccagc gcggcgaata cccgcgagct gtgcggcagc
     1081 acgtagtggc ggtagtcggc cagcgacagc gttcccgccc acgagtcgaa cacctgaatc
     1141 gcgtccaccc cggcttcgat ctgaccgcgc aagaacgcga cggtgagatc ggtcagcttc
     1201 gccatcagcg catgccagct cgccgtttcg gccagcatca tcgccttggt gcgggcgtga
     1261 ttacggctgg ggccgccttc gaccagatag gacgccagcg tgaacggcgc acccgcgaaa
     1321 ccgatcagcg gaacgtcgcc gagctcgccg accagcagcg aaacagcgtc cttgaccggc
     1381 tgaatcgctt gtggatcaag gggtttcatg gcgtcgatat cggcttcggt gcgcaccggg
     1441 tgggcgatca ccggaccgac gtccgcgacg atgtccaagt cgacgcccgc ggcgcgcagc
     1501 ggcaccacga tgtcggagaa caagatcgcc gcgtcgacgc cgtaacgacg tatcggctgc
     1561 agcgtgatct cgcaggccat ctcgggctcg aagcacgccg ccagcatgct gcgccgttcg
     1621 cgcagcgccc ggtattccgg cagtgaacga ccggcctgcc gcatgaacca caccggcacc
     1681 tgggtgggtt tgcgcccggt gagggcggcc agatacggcg actcgggaag ctcgcggcga
     1741 gcgcgcgggg gaatatcggg attgctcatt ggcctcaatg ctgccacgag gccccgaccg
     1801 cacgccccgc tcgcgccgcg ggcggtccgg gagcggccga cgccgctgcg taacatctgc
     1861 ctgaggtcaa ggagcgcaag gcctagggtg cagaagcgca aggcagatgt cagggggtgc
     1921 cgatgccgat cagttacgag ggattccccg gcctgcgctg cgaacccggc gacgaaaccg
     1981 gcctcgacgg cgtgctgacg ctggttctcg acgccccggg gctcaatgcg gtcgggcccc
     2041 aaatgcaccg cgacctggcc gacatctggc cggtgatcga tcgcgatccc gccgtgcgcg
     2101 ccgtcctggt gcgcggtgaa ggcaaggcct tttcctccgg cggcagcttc gagttgatcg
     2161 cggaaaccat cggcgactac gaaggccggc tgcgcatcat gcgcgaggcc cgcgacctgg
     2221 tgctcaatat gatcaacttc gacaagcccg tggtctcggc gatccggggg cccgccgtcg
     2281 gcgcggggct ggtggtgggc ctgcttgccg acatctcggt cgcgggccgg accgcgaagc
     2341 tcatcgacgg gcacaccaaa ctcggcgtgg ccgccggcga ccacgccgcg atctgctggc
     2401 cgctgctggt cggaatggcc aaggccaagt actacctgct cacctgcgag acgctgctgg
     2461 gcgaggaggc cgagcgcatc ggcctggtct ccacctgcgt cgacgacgat gacgtgctgc
     2521 ccaccgccac ccgcctggcc gaaaacctgg cgcagggcgc gcagaacgcg atccgctgga
     2581 ccaagcacag cctgaaccac tggtaccgga tgttcgcccc cacgttcgaa acgtcgctcg
     2641 gcctggaatt cctcggtttc agcggccccg acgtccagga gggcctggcc gcgcatcgcg
     2701 agaagcgccc ggcacgattc accggcggaa acaccacctg agcaggtcga atagcccgca
     2761 agggcgaggt caacgcccag agaatggctg ggtcgcgacg ggcgcccgac acggcgcgcc
     2821 tgtcgcggcg tgtcgggcga tgggtctacc gtcgaacgat gtgacctgcg ccgcgacgat
     2881 gcagagcgat tgcgatgctg aggagcgacg ccaaacggcc agcgccgcga cgatgcagag
     2941 cgatagcgat gccgaggagc gacgccaaac ggccagcgcc gcgacgatgc agagcgacag
     3001 cgatgccgag gaccggcgcc gaaaggcctg cgccgcgacc acgcagaacg gcgcgatgag
     3061 aaggagcagg cgccagtgac gtcagctgaa ccggccccat tcagcgaggc ggtggcggcg
     3121 atgaacgccg tgaccgtgcg gccggagatc gagctgggcc cgatccggcc gccgcagcgg
     3181 ctggcgccct acagctacgc gctgggggcc gaagtcaagc atcccgacct cgagatcgtc
     3241 cccgaacgct ccgagggtga tgcgttcggc cggctgatcc tgctgtatga ccccgatggc
     3301 gccgacgcgt gggacggcac cctccgcctg gtcgcctaca tccaggccga cctggacccc
     3361 agcgaggccg tcgatcccct actgcccgag gtggcgtgga gctggctggt cgaggcgctg
     3421 cagtcgcgga tggagctcgt cacggccctg ggcggcaccg tcaccgccac cacgtcggtg
     3481 cgctacggcg acatctccgg gccgccgagg gcccatcagc tggagctgcg ggcatcgtgg
     3541 acggcgacca cgccccaagt cggggtgcac gtcgaggcgt tctgcgaggt gctcgagcac
     3601 gccgcgggcc taccgccagc cggggtcacc gacctgggct cgcggtcacg cgcctgagat
     3661 gtgcgaaccg gcggccaagg tgcccgaccg caccgcgccc gagagcgccg aatccagccc
     3721 caccccgcta cggcacccgg ccgacgggat gcccgatctc tccgtgaccg tccgcgagat
     3781 cgaggcggcg gctcaactcc tcgacggtgg gcgcggaccg ttcgcggtgg acgccgagcg
     3841 ggcgtcgggg ttccgctact ccaaccgcgc gtacctgatt cagatccggc gcgcccacgc
     3901 cggcaccgtg ctgatcgacc cggtcagcca cggcgccgac ccgctgaccg ccctgcggcc
     3961 cgtcgccgac gtgctcagca ccgacgagtg gatcctgcac tccgccgatc aagacctgcc
     4021 ctgtctggcc gaggtcggca tgcggccgcc ggcgctctac gacaccgagc ttgccggacg
     4081 gctcgccggg ttcgaccggg tgaacctggc gaccatggtg gagcggatgc tgggttttgg
     4141 gttgaccaag ggccacggtg cggcggactg gtccaaacgc ccgctgcccg ccgactggct
     4201 caactacgcg gcgctggacg tggaattgct catcgagctg cgcgcggcga tctcggatgt
     4261 gctggccgag caaggcaaaa cggattgggc cgcacaggaa ttcgactatc tgcggacctt
     4321 caaagcggga gatgctgccg gcgaggttgc cccggccgcg cggcgggagc gatggcgacg
     4381 aacgtcgggg atccatcggg tgcgcgaccc gcgagccctg gcctcggtcc gcgaactgtg
     4441 gacgacgcgc gaccacatcg cccagcgccg cgacatcgcg ccccgccgca tcctgccgga
     4501 ttcggccatc atcgacgccg ccctggccga tccgaagacg gtcgaggccc tcgtcgagtt
     4561 gccggtgttc ggcgggcgca accaacgccg cagcgcggag atgtggttgg cggcgctgga
     4621 agcggcccgg cagaaccagc acccacccga cgacgccgag ccgccgaacg ggccgccgcc
     4681 gccggcgcgc tggagcagac gcaaaccgga ggccgccgca cggctggagg cggccagggc
     4741 ggcgctggcg gaagtgtcgg agcgggtcgg ggttcccacg gcgaacctgg tctcacccgc
     4801 gttggtgcgg cggctgtgct gggattgggc gggcgcgccg gaccccgtcg aggccgtcga
     4861 agacttcttg tgcgccgggc acgcgcgggc gtggcagcga gaactcgtgg acccggttct
     4921 ggcgcgggcg ctgcagccgc cggcggacac cgaggactaa cgccgatccc gacgtagccg
     4981 tttagcggcc gttattcgag atgctcgcga atctccgctt cagaatcgct gctacccagt
     5041 gcggcaaccc agccggtgac ccggcgggcc atgtcctggt cggtcagccc cagatcggcc
     5101 agcacctcat cccgcgaggc gtgcgcgtag aaccgctgcg ccagtccgac gtcgcggcag
     5161 ggcgtatcga tctccgcgct ccgcagcgcg gccgacaccg cggaacccac cccgccgttg
     5221 accccgttgt cctcgcacgt gacgaccaat ttgtgctgcg ccgccagttc gagaacacct
     5281 tcggacaccg gcaacaccca acgcgggtcg atcaccgtca cgccgattcc ctggttgtgc
     5341 agccgcttgg ccgccgccag cgccatcggc acgaacgccc cgaccgcgac cagcagcacg
     5401 tcgtgattca ccccggcggc cggcagcgcc agcacgtcga tacccgagac gcccgacccg
     5461 cgccgctcca gggccggaat gtcttcgccc acatcacctt tcgggaaccg aatcgccgtc
     5521 gggccgtcgt cgacgtccag ggcctcgccg agttcctcgc gcaaccgggt ggcgtcgcgg
     5581 ggtgcggcca cccgcatccc gggcacgatg ttcaacatcg acaggtccca catgccgttg
     5641 tggctggcgc cgtccgaacc ggtgaccccg gcccggtcga gcaccatggt gacgggcagc
     5701 ttgtgcaacg cgacgtccat catgatctgg tcgaaggccc ggttgaggaa cgtcgagtag
     5761 atggctacca ccgggtgcat gccgcccatc gccagcccgg ccgccgacgt caccgcgtgt
     5821 tgctcggcga tgcccacgtc gaacagacga tccgggaacc gctgcccgaa cgccgtgaga
     5881 ccggtggggc cgggcatcgc cgcggtgatg gcgacgatgt cgcggcgctt cttcgcgtag
     5941 ccgatgagcg cgtcggaaaa ggtcgccgtc cagccggggc cggcgatctt ggtggcctgg
     6001 ccggtggccg ggtcgatcac gccgcaggaa tgcatctgct cggcctcgtc gtcctcggcg
     6061 gggcgataac ccatgccctt gcgcgtcaca acgtgcacga tcaccggccg cccgaagccg
     6121 cgggcgtggc gcagcgcggc ctccaccgca tgttcgtcat gaccgtcgac cgggccgacg
     6181 tacttcagcc cgaggtcggt gaacaggagc tggggtgaca gcgaatcctt gatgccggct
     6241 ttgacgctgt gcacgaagtg gtaggcgacc tcgccgatga cgggcatgga gcgcaacgcg
     6301 tcgcggcccc gctccagcaa ctgctcgtag cccggctgca gccgcagcgt agccaggtgg
     6361 tcggccaccc cgccgatcgt gggcgcgtag ctgcggccgt tgtcgttgac cacgatgacc
     6421 accgggcggc tagaggcggc gatgttgttc agcgcctccc agcacatgcc gccggtgagc
     6481 gcgccgtcgc ccaccaccgc gaccacatgc cgattgcggt gtccggtcaa ctcgaacgct
     6541 ttggccagac cgtcggcgta cgacaacgcg gcgctggcgt ggctggactc cacccagtcg
     6601 tgttcgctct cggcgcgaga cggatacccc gacaggccgc ccttcttgcg caggccctcg
     6661 aagtcgaggc tgcggcccgt caatatcttg tggacatagg cctgatggcc ggtgtcgaag
     6721 atgatcgggt cgtgcggaga gtagaacacc cggtgcagcg ccagcgtcaa ctcgacgacg
     6781 ccgaggttgg gccccaggtg tcccccggtg gcagcgacct tgtggatcag gaactcgcga
     6841 atctccgcgg ccagatcgcg tagctgctgt tgggacaggt gctgcagatc agcgggcccg
     6901 cggatccgtt ccaacatccc gctagtttac gcagcgaccg ccggatcact agttctgaac
     6961 gggccgcgac gtattgtctc cactatgcgc gccgcgcaac tcgccgagga ctttccagtc
     7021 gtgagcgtcg actccgacgc cctcgatgcc gcccgcctgc tcgccgagca ccgcctgccc
     7081 ggaatagtcg tcactgacag gtccggagaa ccgttcgcgg tgctgccggc ttcccaggtg
     7141 gtgcggttca tcgtgccccg atacgtgcaa gacgacccct cgctggccgg cgtgctcaac
     7201 gaatcgatgg ccgaccgcgc ggccgacaag ctgggcggca agacggtcgg cgacatgctg
     7261 ccggaccacc tcaccaatgt gccgtgggcg cacgccgacg acacgatcat cgaggtggcc
     7321 gcggtcatgg cgcggctgcg cagcccgctg atcgcggtcg tcaaagacaa ggcactggtc
     7381 ggcgtcatca cggcgtcgcg tctattggct gcggctttaa aacactgatc ctgccgaagc
     7441 gttgagcaca caggacatcc gatgagcgtc gtcgcggtcg tcgtgttcgt cgtcgcctac
     7501 gcgctgatcg ccagtgaccg cgtcaacaaa acgttggtgg cgctgacggg cgcggcgatc
     7561 atggtcgcat tgccggtgat cgattccgac gacgtgttct actcccgcgc caccggaatc
     7621 gattgggacg tcctgttttt gctgctgggc atgatgatca tcgtcagtgt gctccggcag
     7681 accggtgtgt tcgaatacac cgcgatctgg gccgccaagc gcgcccacgg ctccccgctg
     7741 cgcatcatga tcctgctggt ggtggtcacc gcgttcgcgt cggcgttgtt ggacaacgtc
     7801 acgacggtgt tgctgatcgc cccggtcacg ctgctggtgt gcgaccggct cgcgatcggt
     7861 ccggcgccgt ttttgatggc cgaggcgttc gcgtcgaaca tcgggggcac cgcgaccctg
     7921 atcggcgacc cgcccagcat catcatcgcc agccgggccg gcctgagctt caacgacttt
     7981 ctgatccatc tgaccccgat agtgatcgtc atcaccatcg tcttctgcgt cttgttgcca
     8041 cgcctgtttc cgggcgcgtt cgccgtcgat ccggagcggg tcgccgacgt catgtcgctg
     8101 gaagaacgcg aggccatccg cgatccgcgg ctgctcatca agtgcggcgt tgtcctgatg
     8161 gcggtcctcg ccgggttcgt cgcccacgcg ccgctgcacc tggcaccgtc catcgtggcc
     8221 ctgctgggcg ccggcattct catcgtcagc tcccggttgg agccgtcgga atacctgtcc
     8281 agcgtcgaat gggacacgct gctgttcttc gccggactgt tcgtcatggt cggcgctctg
     8341 gtcaagaccg gcgtcgtcaa ccagctggcg cgcctggcca ccgctgccac cggcgggcac
     8401 gtcttgttga caacgatggt gattctcgcg gcttcgttcg tgttcagcgg cttgatcaac
     8461 aacgttccgt acgctgcgac gatggcgccg atcgtcgcgc aactgatccc gtcgatgggc
     8521 cacgccaaca cgcaggcgat gtggtgggcc ctggtcctcg gcaccgatct cggcggcaac
     8581 ctcaccgcag tcggggccag cgccaacgtc gtcatcctcg gactcgccag gcgcgccgac
     8641 agtcccatct ccttttggga attcacccgc aagggcgcgg tcatcacgac gatctccgcg
     8701 gcgctgtccg cgctctacct gtggctgcgg tatttcgcgc tgagctagcc ggtgacccgc
     8761 gtgcctgaag attccgagga tgcctcgatg ggctccggtg tgcatgcctc gccgatgccg
     8821 aaccgcgcat gaagccgggc cagcggcttc ggcgcccacc aattggcgcg gcccagcacg
     8881 cgcatgaatg ccggaacgag cagtatccgc accagcgtcg catccaccag caccgccagc
     8941 gtcaggccga caccgaacat ccgcatcaac gccacctgcg cggcgatcaa cgcggcgaac
     9001 gaaatggaca tcaccagcgc ggcggctgtc accacccgcc cggtgcgggc caggcccagc
     9061 gcgacggttt catcgttgtc ggcattaggg cttccggaca tcaaccagta ctcgcggatg
     9121 cgcgaaacca gaaacacttc gtaatccatc gacagtccaa atgcgatgca gaacaacagc
     9181 accgggatgg ttgcgaccag cgtcccggta gcggtggtgc cgaacgcgcc cagatgaccg
     9241 tcctggaaga tccacaccag agcaccgaag gtggcggaca gcgataagac gttgagcagc
     9301 aacgccttca acggcaacac cacgctgccg gtcagcaaga acagcagcac gaacgtgatc
     9361 gcggcgatca ccgccagtac ggcgggcagc cgcgaggtga tcgcgtcgca actgtcgcgg
     9421 ctgatctgcg ctattccggt aatctgcacg tgttggccgc cgggaccggt gagggcgtgc
     9481 aaccggtcca actgaatctc cgaagcccgt gagaacagcg gcgcggtgct gccaatcgtg
     9541 aaaaatgcgc tgccgtcctt gatcgctgcc gccgacggcg gcccgaccaa cgagccggcc
     9601 acgaacgtgc cgcccggcgc cgacaccgat gacacgtcag gaacccgcga cagctgcacg
     9661 gcatagctgt tgagttcgcg gggtgtgacg gtagtcaagt ccggaatcac gacggtcacg
     9721 ttggtcgccg agtcgacggc aaaagcgccg cgcacctggt caccgacctg gcgcgccgac
     9781 gcggagtcgg gcagtacccg atcgtccgga aaaccccatt tgatgccgag gaagggcgcg
     9841 ccaagtatca gcagcagcgc gatgatcgcc aggccgatcg cgatggcgtg ccgcatcacg
     9901 aacttggtcg agcggtaaaa gaacgtctgc tcgaccgggc gtcgtgccgg ctccgcccgt
     9961 ctgagcagcc ggcggcccag tcggcgcacg tccactgagt ccagccggct cccgagcagc
    10021 acgatcgcag ccggcgtcac cacgaccgcg gcaacggccg ccgacgccac cacggcaatg
    10081 ccggcgtagc cgaaagattt cagaaagtac atcgggaaca gcatcagagc catcatcgac
    10141 agcccgaccg tcatcgccga gaacagcacc gtccggccgg ccgtggtcat cgtgcgaacc
    10201 agcgcctggt cccgatccgc gccctcggcg agttcgtcac ggaaccggct gacgatcagc
    10261 agcgtgtagt cgatggccaa cgccaaactc attgcgacag tgaggtttag cgcgtagatc
    10321 gacacgtcgg ttgcgtacgt gatcgcgcgc agcaccgcca tcgaagtcag gatcgcgaac
    10381 ccgcccaccg ctagtggcag cgccgccgcc atgacgccac cgaacaccca gaccagcacc
    10441 acgaagctca acggcatagc gatggattcc attagcagca agtctttttc gctttggcta
    10501 ttgacctgga cgcgcgtcat cgcgtcgcca ccagcctgga cggtgacccc gtcgcggtcg
    10561 tacaccagct gatcggccag ctgcttggca tgctgctgcg cgccgtcctc gccgccggtg
    10621 atgccggcaa cgatcagccc ggtcttgccg tccttgctga tca
//
LOCUS       DASSGQ010000238         7919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_533349,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000238 DASSGQ010000000
VERSION     DASSGQ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7919
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533349"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="VFI55_10325"
     CDS             <1..1497
                     /locus_tag="VFI55_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020731885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HET7074461.1"
                     /translation="DVRAMLADRLAGRPTRANFRTGTLTVCTMVPMRSVPHRVVCLVG
                     LDDGVFPRLGVVDGDDVLARDPMTGERDIRSEDRQLLLDAIGAATEKLVITYTGANEY
                     SGQPRPPAVPLAELLDTLDVTVPNNRRGNIRERIVVKHPLQPFDVRNVMPGELVPKVP
                     FSFDPTVLRAARVAVGQRGEMPQFISRPLPPLPADDVILADLVEFFKDPVKGFFRALG
                     FTLPRDVDGVADAMPVDINSLEEWTVGDRMLGDILSGMTPDDARQAEWRRGTLPPGQL
                     GWRKATEIRDQAALLAMAARQYRQADARAYDVNVDLGSGRRLTGTVSPVYGARLVSAT
                     YSRLDGKHLLESWIPLLALIAHHGARAWSAVCIGRPKRGATPRVAALGPPQGDAADVL
                     RELVAIYDAGRREPIPLPIKTSYAWAAARHCGDDPVREARYRWKSERYPAEDQAPAHV
                     QAWGRAARLTDLMQPVRPGEEFDGEDNRLGAYAARLWLPLLRAEKAPT"
     gene            1497..4814
                     /gene="recB"
                     /locus_tag="VFI55_10330"
     CDS             1497..4814
                     /gene="recB"
                     /locus_tag="VFI55_10330"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HET7074462.1"
                     /translation="MDRFNLLGTLPAERSTTVLEASAGTGKTFALAGLVTRYVAEGQA
                     TLDQMLLITFSRAASQELRERVRSQIVHAVHAFEDPAAIGGNQLVAHLLTGTDDERAL
                     RRQRLRDALAGFDAATIATTHQFCQLVLKSLGVAGDTDAGVTLLESLDELVVEIVDDL
                     YLAHFGRERNDPALAYADALRLAREVVKNPSTQLRPRDPQPGAHAAVCVGFAKDVLAE
                     LDTRKRRQGVLGYDDLLSRLADALATEDSPARVRMYQRWPIVMVDEFQDTDPVQWQVI
                     DRAFSGRSTVILIGDPKQAIYAFRGGDIATYLRAAETAGEKKTLATNWRSDAALIERL
                     QVVLRGAQLGDSAIVVDDVQAHHQGHRLAGAPRNDPFRLRVVRRAALGRSGIQNLPIE
                     ELRAHIGADLAADIRALLANGATFAGRRLQAHDIAVIVAKHKDARACFKALCDAGVPA
                     VYTGDSDVFASKAADDWLCLLEAFDQPHRPGLVRAAASTMFFGETAESLAAGGDALTD
                     RLAETLREWAGHARERGVAAIYEAAQLRGMGDRVLSWQDGERHMTDLAHIAQLLQDVA
                     HREHHSLPALRDWLRSQREERSGATEHNRRLDSDAAAVQIMTVFVSKGLQYPIVYLPF
                     AFNRNTQDRDVVLFHDEGVRCLHIGGRDSPDFRAVEMLGRREDASDDSRLTYVAMTRA
                     QSQVVAWWSPAFDEPNGGLSRLLRGRRPGESLVPDRCEPAKISDDDAMARLREWEAAG
                     GPVIEDAAIAVAPSLAPAPTPTHLASRHFHRSIDTAWRRTSYSSLISAAETTGVSSEP
                     EVTELDDEAGEIPLTEAETETLTETETGLLSPMADLPRSAQFGSLVHAVLETADPLAA
                     DLGAELQAHIRRHSVWWPVPVAPEVLAAALVPMHETSLGPLAGGLTLRQIGLRDRLRE
                     LDFELPLGGGELRSHPPDIRLADVGMLLREHLPTDDALASYADRLTGRALGGQSLRGY
                     LTGSVDAVLRVPGAAGHRYLVVDYKTNWLGDPERPLTAADYQRPRLVEAMLHSDYPLQ
                     ALLYCAVLHRFLRWRVPDYDPARHLGGVLYLFVRGMCGADTPVLDGQPCGVFSWQPPS
                     SLVAALSDLLDAGRAAA"
     gene            4811..6544
                     /gene="recD"
                     /locus_tag="VFI55_10335"
     CDS             4811..6544
                     /gene="recD"
                     /locus_tag="VFI55_10335"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HET7074463.1"
                     /translation="MTVEAIDAAGLLRPFGDAGVFEAADIHVAQRLTELAGEPDERVA
                     LALALVVRALRGGSVCVDLRAAPAQVGVADLPWPDAESWMIAVRASPLLGSPPVLRLL
                     GDLLYLDRYWLEEEQVCVDLLALSVSAAIGDTPAFERLFPPGYDEQRAAAEIALSQAV
                     TVLTGGPGTGKTTTVARLLALLAGQAERAGAGRPRIALAAPTGKAAARLAEAVAAEVQ
                     QLEPVDRARLAGLQATTLHRLLGSRPDTSVRFKHNRGNRLPHDVIVVDETSMVSLTMM
                     ARLLEAVRPGTRLILVGDPDQLASVEAGAVLADLVEGLRARRDGRVAALRTKHRFGES
                     IGALAEAIRTGDADRAVESLRAGGRHIEWIEPGGSADRLRAVLVPHALRMREAAVLGA
                     AEVALRTLDEHRLLCAHREGPYGASHWNRQVQRWLAEETGQPMPLEWYAGRPLLVTAN
                     DYGLKVFNGDTGVVVAGQDGSRDGLRAVIAGATGPLFFATGRLADVETMHAMTIHKSQ
                     GSQAEEITVLMPPEDSRLLTRELFYTAVTRAKVKVRVVGSEAEVRAALSRRAVRASGL
                     RLRLQAAAATA"
     gene            complement(6559..>7919)
                     /locus_tag="VFI55_10340"
     CDS             complement(6559..>7919)
                     /locus_tag="VFI55_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019695602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HET7074464.1"
                     /translation="IGTVTAVEPTEHGARATMSIDNGYQIPTDASANVHSVSAVGEQY
                     LDLVSTRTGGPYLADEQTIVKTTVPSQIGPALDAANRGLAALPKDKVASLLRETSQAV
                     GGLGPSLRRLVDATQAIAHDFRGSIDDVDDIIANSAPIIDSQVNSGDDIAHWAANLNT
                     LTAQTAQQDQALHSILANAAPTADQVNDTFSDVRDALPQTLANLEVVIDMLKRYHNGV
                     EQALVFLPQSGAIAQSVTSEFPGQAALGVGALSLNQPPPCLTGFLPASEWRPTADTTI
                     APLPAGSYCKIPMDATNVVRGARNYPCVDVPGKRAASPRECRSKEPYVPLGTNPWYGD
                     PDQILTCPAPAARCDQPVKPGQVIPAPSVSNGVNPAPADRVPGTPPPVSDPLQRPRSG
                     TVQCNGQQPNPCIYTPSAPPTSIYDVQSGLVVGPDGVVYSVENSKNTGEDGWREMLAP
                     AH"
BASE COUNT         1164 a         2601 c         2883 g         1271 t
ORIGIN      
        1 gacgtacggg ccatgctcgc cgaccggctg gcaggccggc cgacgcgcgc caacttccgc
       61 accggcaccc tcaccgtgtg cacgatggtg ccgatgcgct cggtgccgca ccgagtggtg
      121 tgcctggtcg gcctggacga cggcgtgttc ccgcgcctag gtgtcgtcga cggcgacgac
      181 gtgctggccc gtgacccgat gaccggcgaa cgcgatatcc gttcggagga ccggcaattg
      241 ctgctcgacg ccatcggggc ggccaccgag aaattggtga tcacctacac cggggccaac
      301 gagtattcgg gccagccacg cccgcccgcg gtgccgctgg ccgagttgct ggacactctc
      361 gacgtgaccg tgccgaataa ccggcggggg aacatccgcg agcgcatcgt cgtgaagcat
      421 ccgttgcagc ccttcgatgt tcgcaacgtc atgcccggcg agctggtgcc gaaagtcccg
      481 ttcagcttcg acccgaccgt actgcgggcg gcgcgggtgg ccgtcggcca acgcggcgaa
      541 atgccgcagt tcatttctcg gccgctgcca ccactgcccg ccgacgacgt catcctcgcc
      601 gacctcgtcg aattcttcaa agacccggtg aagggattct tccgcgcact ggggttcacg
      661 ctcccgcgcg acgtcgacgg tgtggcagat gcgatgccgg tcgacatcaa cagcctcgag
      721 gagtggacgg tcggcgaccg gatgctgggc gacatcctga gcgggatgac gccggatgac
      781 gcacgccagg cggagtggcg ccggggcacg ctgccgccgg gccagctggg gtggcggaaa
      841 gcaaccgaga tccgcgatca ggccgccctt ttggcgatgg cggcgcggca gtaccgccag
      901 gcggacgcgc gggcgtacga cgtcaacgtc gacctcggct ccggacgacg gcttacgggc
      961 acggtgtcgc cggtgtacgg cgcgcggctg gtgtcagcga catattccag gctcgacggc
     1021 aagcacctgc tcgagtcctg gattccgttg ctggcattga tcgctcacca cggtgcccgc
     1081 gcctggtcgg cggtgtgcat cggccgcccc aagagaggcg ccaccccgcg ggtggccgcg
     1141 ctgggcccgc cccaaggtga cgccgccgac gtgctgcgtg agctggtggc gatctacgac
     1201 gcagggcgcc gggagccgat tccgttgccc atcaagacgt cgtatgcgtg ggcggccgcc
     1261 cgccattgcg gcgacgatcc ggtacgcgag gcgcgctacc ggtggaagtc cgaacggtac
     1321 cccgcggagg atcaggcgcc cgcccacgtg caggcatggg gcagagccgc gcggctgacc
     1381 gacctcatgc agcccgtgcg gcccggcgag gaatttgacg gcgaagacaa ccggctcggc
     1441 gcctatgccg cccggctttg gctgccgctg ctgcgcgccg aaaaggcccc gacctgatgg
     1501 atcgcttcaa ccttttgggc acgctgcccg ccgaacgctc caccacggtg ctggaggcga
     1561 gcgccggcac cggcaaaacc ttcgcgttgg ccggcctggt gacccgctac gtcgccgagg
     1621 gccaggcgac gctggatcag atgctgctca tcacgttcag ccgggcggcc agccaggagc
     1681 tgagggagcg tgttcgctcc caaatcgtgc atgctgtaca cgctttcgag gatccggccg
     1741 ccatcggcgg caaccagttg gtggcgcacc ttctgacggg caccgatgac gagcgcgcgc
     1801 tgcgcaggca gcggctgcgc gatgccctgg ccggcttcga cgcggcgacg atcgccacca
     1861 cgcaccagtt ctgccagctg gtgctgaaat ccctgggggt ggccggcgac accgacgccg
     1921 gtgtgaccct gctcgagagc ctcgacgagc tggtcgtcga gatcgtcgac gatctgtacc
     1981 tggcccactt cgggcgggag cgcaacgacc cggcgctggc ctatgccgac gcgctgcgac
     2041 tggcgcgcga agtggtcaag aacccgtcga cacagctgcg gccacgtgac ccgcagccgg
     2101 gcgcccacgc ggcggtctgc gtcggcttcg ccaaagacgt tctcgccgaa ctggataccc
     2161 gcaagcggcg ccagggtgtt ctcggttacg acgacctgtt gagccgactt gccgacgcgc
     2221 tggccaccga agactctccc gcacgggtgc gcatgtatca acgttggccg atcgtcatgg
     2281 tcgacgagtt ccaggacaca gacccggtgc aatggcaggt gatcgaccgc gcgttcagcg
     2341 ggcgctccac cgtgattctc atcggtgatc cgaagcaggc catctacgcg ttccgcggcg
     2401 gcgacatcgc gacgtatctg cgggcggcag agactgcggg cgagaagaag acgctggcca
     2461 ccaactggcg cagcgatgcg gcgctcatcg aacggctgca ggtggtgctg cgcggcgccc
     2521 aactcggcga ctccgcgatc gtggtggacg acgtccaagc gcaccaccag ggccaccgac
     2581 tcgcgggtgc gccgcgcaac gatccgttcc ggttgcgggt ggtgcgtcga gcggcattgg
     2641 ggcgcagcgg tattcaaaac ttgccgatcg aagagctgcg ggcgcatatc ggcgccgacc
     2701 tcgccgccga tatccgcgcg ctgctggcga atggcgcgac tttcgccggc agacgactgc
     2761 aggcccacga catcgccgtg atcgtggcga aacacaagga tgcccgggca tgtttcaagg
     2821 cgctgtgcga cgcgggcgtt ccggcggtgt acaccggcga ctccgacgtg ttcgcatcga
     2881 aggccgccga cgactggctg tgcctgctgg aggcgttcga ccaaccgcac cgtccgggcc
     2941 tggtgcgcgc cgcggcgtcg accatgttct tcggcgagac cgcggaatcg ctggcggccg
     3001 gcggtgacgc gctgaccgat cggctcgcgg agacgctgcg cgaatgggcc gggcacgccc
     3061 gggaacgcgg cgtcgcggcc atctacgagg cggcgcagct gcgaggcatg ggcgatcgcg
     3121 tgctgtcctg gcaggacggt gagcgccaca tgaccgacct ggcgcacatt gcgcagttgc
     3181 tgcaggacgt cgcgcaccgc gagcaccaca gcctgcccgc gctccgcgac tggctgcgca
     3241 gccaacgcga agaacgcagc ggcgcaacgg aacacaaccg gagactcgac agtgacgcgg
     3301 ccgccgtgca gatcatgacg gtgtttgtca gcaagggtct gcaatacccg atcgtgtatc
     3361 tgccgttcgc gttcaaccgc aatacccagg accgcgacgt ggtgctgttc cacgacgaag
     3421 gcgtgcgctg cctgcatatc ggtggcaggg acagtccgga cttcagggcc gtcgagatgc
     3481 tgggccgcag ggaggatgcc agcgacgaca gccggctgac ctatgtcgcg atgacccgcg
     3541 cgcagtcgca ggtggtggcg tggtggtcgc ccgccttcga cgaacccaac ggcggcctgt
     3601 cgcgactact gcgtggtcgc cggcccggcg agtcgttggt gcccgaccgg tgcgagcccg
     3661 cgaagatctc cgacgacgac gcgatggccc ggctgcgtga gtgggaggcc gcgggtggcc
     3721 cggtgatcga ggacgcggcg atagccgtcg ccccttcgct tgcccccgcg ccgacgccga
     3781 cgcacctggc tagccggcac tttcatcgca gcatcgacac agcttggcgc cgcacctcct
     3841 attcgagtct gatcagcgcg gcggagacca ccggcgtcag cagcgagccc gaggtcaccg
     3901 aactggacga cgaggccggc gaaatcccgt tgaccgaggc tgagaccgag accttgaccg
     3961 agaccgagac cggcctgctg tcgccgatgg ccgacctgcc gaggagcgca cagttcggct
     4021 cactggtgca cgccgtcctc gagactgccg acccgctggc cgctgatctg ggcgccgagc
     4081 ttcaggcgca catccggagg cattcggtgt ggtggccggt gccggtggct ccggaggtgc
     4141 tggccgcggc gctggtgccg atgcacgaga cttcgttggg cccgctggcc ggcggtctga
     4201 cgctgcgaca gatcgggttg cgggatcgtt tgcgagagtt ggacttcgag cttccactcg
     4261 gcggcggcga gctgcggtcg catccgcccg atattcgtct ggcggatgtg gggatgttgc
     4321 tgcgcgagca cctgccgacc gatgacgcgc tggcgtccta cgccgatcgg ttgactggcc
     4381 gcgcgctggg cggccagtca ttgcgcggat acctcaccgg ctcggtggat gccgtgttgc
     4441 gggtccccgg cgcggccggg catcgctatc tggtggtgga ctacaagacc aactggctcg
     4501 gcgatccgga acggccgctg accgccgccg actaccagcg gccgcggctg gtcgaagcga
     4561 tgttgcattc ggactatcca ctgcaggcgc tgctgtactg cgctgtgctg caccggttct
     4621 tgcggtggcg ggtgccggac tacgacccgg cccgccacct tggcggcgtc ctctacctgt
     4681 tcgtgcgggg gatgtgcggc gccgacaccc cggtgctcga cgggcagccg tgcggggtgt
     4741 tcagctggca gccaccgtcg tcgctggtcg cggcgctgtc ggacctgctc gacgcgggga
     4801 gggcggcagc gtgacggtcg aggcgatcga cgccgcggga ttgctgcggc cattcggcga
     4861 tgccggcgtc ttcgaggccg cggatatcca tgtggctcag cgacttacgg agctcgcggg
     4921 tgaacccgac gagcgagtgg cgctggcgct cgccttggtg gtgcgcgcgc tgcggggcgg
     4981 ctcggtgtgc gtggacctgc gcgcggcgcc ggcgcaggtc ggagtggctg acctgccgtg
     5041 gcccgacgcc gagtcctgga tgatcgccgt gcgggccagt ccgctgctcg gatcgccgcc
     5101 ggtgctgcgg ctgctcggcg atctgctgta cctggaccgg tactggctcg aggaagagca
     5161 ggtgtgcgtc gacctgctgg cgctgtcggt gtccgcagca atcggcgaca caccggcatt
     5221 cgagcggctg ttcccgccgg ggtatgacga gcaacgggca gccgccgaaa tcgcactgtc
     5281 gcaggcggtt accgtgctca ccggaggacc cggcaccggc aagaccacca cggtcgcgcg
     5341 gctgctggcg ctgctggccg ggcaggccga gcgcgccggt gctgggcggc cccgaatcgc
     5401 cctggccgca ccgaccggta aggccgccgc gcggctggcc gaggcggtgg ccgccgaggt
     5461 gcagcagctc gagccggtgg acagggcgcg gctggccggc ctgcaggcga caacgctgca
     5521 ccggctgttg ggctcccgac cggatacgtc ggtccggttc aagcacaatc gcggcaaccg
     5581 gctgccgcac gatgtgatcg tcgtcgacga gacgtcgatg gtgtcgctga cgatgatggc
     5641 ccgcttgctg gaagccgtac gcccgggcac gcggctgatc ctggtgggcg atcccgacca
     5701 gctggcctcc gtcgaagccg gtgccgtgct ggccgacttg gtggaggggc tgagggctcg
     5761 gcgcgacggg cgggtggctg ccctgcgcac gaagcatcgg ttcggggagt cgatcggtgc
     5821 actggccgaa gcgatccgca ccggcgacgc agaccgtgcc gtggagtcgc tgcgggccgg
     5881 cggccggcat atcgagtgga tcgagccggg cggctcggcc gatcggctgc gtgcggtgct
     5941 ggttccgcat gcgctgcgga tgcgcgaggc tgcggtgctc ggcgccgccg aggtggcgtt
     6001 gcggacactc gatgagcacc ggctgttgtg tgcgcaccgg gagggaccgt acggggcctc
     6061 gcattggaac cggcaggtgc agcgctggct tgcggaagaa acgggccagc cgatgccctt
     6121 ggagtggtat gccggtcggc cgctgttggt gacggcgaat gactatgggc tgaaggtctt
     6181 taacggtgat accggggtgg tcgtcgccgg ccaggacggt tcgcgggacg gcctccgtgc
     6241 cgtcattgcc ggtgccaccg ggccgctgtt tttcgcgacc ggccggctgg cggacgtcga
     6301 gaccatgcat gcgatgacga tccacaagag ccagggcagc caggccgagg agatcaccgt
     6361 gctgatgccg cccgaggatt cgcggttgct gacccgcgag ctgttctaca ccgcggtgac
     6421 ccgggccaaa gttaaggtcc gggtggtcgg ctcggaggcc gaagtgcgcg ccgcgctaag
     6481 tcgccgcgcg gtccgggcaa gtgggctacg gctgcggctg caagcggcag cggctaccgc
     6541 gtgacggcgg gcctggattc agtgggccgg cgccaacatc tcccgccacc cgtcttcgcc
     6601 cgtgttcttc gaattctcaa cggaatacac cacaccgtcg ggtcccacga ccaagccgct
     6661 ctgcacgtcg taaatggagg tgggaggcgc gctcggggtg tagatgcagg ggttgggttg
     6721 ctgtccgttg cactggacgg ttcccgaccg cggtcgctgc aacgggtcgc tgacgggcgg
     6781 tggtgtgccc ggcacccgat cggcgggtgc cgggttgaca ccgttactga ccgacggcgc
     6841 cgggatcacc tgtcccggct tcaccggctg gtcgcaacgc gcggccggcg cggggcacgt
     6901 gaggatctga tctgggtcgc cgtaccaggg gttggtaccc agcggcacgt agggctcctt
     6961 gctgcggcac tcccgagggc tcgccgcccg cttgccgggc acatccacgc acgggtaatt
     7021 gcgtgctcca cgaaccacat tcgtcgcgtc catcgggatc ttgcagtagc tgcctgcggg
     7081 tagcggcgca atggtggtgt cggccgttgg ccgccactcc gatgcgggca ggaagccggt
     7141 caagcatggt ggtggttggt tgagggacag ggcgccgacg ccgagtgccg cctggccggg
     7201 aaattccgag gtaaccgact gggcgatcgc gcccgactgg ggcaagaaca ccagcgcctg
     7261 ctcgacgcca ttgtggtagc gcttgagcat gtcgatcacg acctcgagat tggccagcgt
     7321 ctgcggcaac gcgtcccgca cgtcgctgaa tgtgtcgttg acctgatcgg cggtcggcgc
     7381 cgcgttggcc agaatgctgt gcagggcttg gtcctgctgc gcggtctgcg cggtcagagt
     7441 gttcagattg gcggcccagt gcgcgatgtc gtcacccgag ttgacctggc tgtcgatgat
     7501 gggtgccgaa ttcgcgatga tgtcgtcaac gtcgtcgatg ctgcccctga agtcgtgggc
     7561 gatcgcctgg gtggcgtcga cgaggcgcct cagggacggt cccagcccgc ccaccgcctg
     7621 cgacgtctcg cgcagcagcg acgccacctt gtctttgggc agcgccgcca atccgcggtt
     7681 ggcagcgtcc agcgccgggc cgatctggct ggggaccgtc gttttgacga tcgtctgctc
     7741 gtcggccagg tacggaccgc cggtgcgggt cgacaccagg tcaaggtatt gctcgccgac
     7801 cgccgacacc gagtgcacat tggccgacgc atcggtcggg atctggtagc cgttgtcgat
     7861 gctcatcgtt gcgcgcgcgc cgtgctcggt gggttcgacg gcggtgacgg tgccgatgg
//
LOCUS       DASSGQ010000239        31856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1414378,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000239 DASSGQ010000000
VERSION     DASSGQ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 31856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31856
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414378"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..1014
                     /locus_tag="VFI55_10345"
     CDS             193..1014
                     /locus_tag="VFI55_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HET7074465.1"
                     /translation="MSQSISGKVAFITGAARGQGRAHAVRLSAEGADIIALDIAGPLP
                     SSVPYDSPTPDDLAETGRLVRANGRKVVTAAVDIRDLDALKAAVDNAVAELGRLDIIV
                     ANAGICSPAPWDQITPEAFRDTIDTNVVGTWNTVMAGAQHMIDGGRGGSIILIGSAAG
                     INMQSFMIHYTASKHAVVGMARAFAAELGRHNIRVNSLNPGAVATPMGTGRMREALHS
                     AADSYPHLRGMHKPLLPEGIAQPEDIADAVAWLASDQSRLVTATQLSVDIGVGYV"
     gene            1167..1379
                     /locus_tag="VFI55_10350"
     CDS             1167..1379
                     /locus_tag="VFI55_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HET7074466.1"
                     /translation="MTTSEYRVSGMSCRHCEAAIENEVGQIPGVHGVDVSARTGRLVV
                     TSSAHIDAKAVLDAVDEAGYAAVPVA"
     gene            1376..3655
                     /locus_tag="VFI55_10355"
     CDS             1376..3655
                     /locus_tag="VFI55_10355"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET7074467.1"
                     /translation="MKAPDTTGSPTTAGSIELAITGMTCASCAMRIEKKLNKFDGVDA
                     AVNYATEKATITIPPGFDPLTLIAEVEKAGYAAALPQHRRRGPETPQVTDDAELTSLR
                     ARLVAAIVLAGPVIAMAMMPALQFRYWQWVSLGLATPVIVWAGRPFHTAAWVNLKHGA
                     ATMDTLVSVGTLSAFLWSLYALFFGTAGQPGMRHGFDLTVARSDGAGNIYFEVAAGVT
                     LFVLAGRYFEKRSKRRAGAALRALLQLGAKDVAVLRDGTETRIPVEQLAVGDEFVVRP
                     GEKIATDGTVVSGSSAVDASMLTGESVPVEVGQGDTVAGATVNAGGRLVVRATRVGDD
                     TQLAQMAKLVEAAQSGKADVQRLADRISGVFVPVVIAVAVATLGVWLVAGSSVTAALT
                     AAVAVLIIACPCALGLATPTALLVATGRAAQLGILIKGPEVLESTRRVDTIVLDKTGT
                     VTTGRMTLVDVITSPGTDRETLLRYAGAVEDASEHPVAQAIARGAKAELGTLPGVEGF
                     TAVAGNGVHGTVDGHVVVVGRESLLADWAQHLDSDLSAAKAGAEGEGKTAVVVGWDGR
                     AHGILMIADTVKPTSAQAIRQFKALGLTPVLLTGDNATVAARIADEVGITHVIAEAMP
                     ADKVAAVQRLQAEGKVVAMVGDGVNDAAALATADLGLAMGTGTDVAIEAADLSLVRGD
                     LGAAADAIRLSRRTLRTIKTNLFWAFGYNVAAIPLAALGLLNPMLAGAAMVFSSVFVV
                     GNSLRLRSVTTTMRADPVG"
     gene            3749..5140
                     /locus_tag="VFI55_10360"
     CDS             3749..5140
                     /locus_tag="VFI55_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET7074468.1"
                     /translation="MPNFVEQLSLLHGWLRPTAQALALVVAAGAIGWRRRRWRSWALV
                     AALGVAALVTALSYWYITSLGVAGDPAPASLWLWIAMSGLAIAVLLLGWPGARWWRRA
                     MAVLLVPLCALCAALALNGWVGYFPTVLAAWNQLTSGPLPDQIDRLRVTEMQVAGTRP
                     AKGVVVPVNINSNASHFPHRPELVYLPPAWFSSNPPPRLPTVMMIGSAFNTPADWLRA
                     GGAFDTIDNFAAHHQGFAPALVFVDATGAFDNDTECVNGSRGNAADHLTRDVVPFVVS
                     NFGVSADRANWGLAGWSMGGTCAVDLAVTHPDMFSAFVDIAGDRSPNVGTKEQTLARL
                     FGGNTAAWAAFDPVTVMNRHGHYTGVSGWFDVPASSGPRNLAQEASPGLTTSSTDPAA
                     NPEGQDAAANTLCTAATANGITCAVVTQPGKHDWPFAAQAFAASLPWLASTLGTPSVE
                     PVRLPQRAGGAPH"
     gene            complement(5165..6010)
                     /locus_tag="VFI55_10365"
     CDS             complement(5165..6010)
                     /locus_tag="VFI55_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET7074469.1"
                     /translation="MTTAFVLSGGASLGAVQVGMLRALVGEGITPDLIVGTSVGALNG
                     AWVASRPDAAGIDALADLWLSLSRKEIFPTHLVGGLMGFLGRRPHLIPSSGLRRLLSE
                     QLGFTRLEDAPIPFHVVATDVISGTDVLLSSGDAVDAVAASAAIPGIFPPVTINGRGL
                     MDGGVVNNTPLSHAVALGADRVWVLPTGYSCDLPALPQSALTMALHALTLTINHRLAV
                     DVARFEQAVELHVVPPLCPIATSGADFSHAATLIEQSHAATREWLAACPPSTGQARLL
                     EPHRH"
     gene            6388..7197
                     /locus_tag="VFI55_10370"
     CDS             6388..7197
                     /locus_tag="VFI55_10370"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7074470.1"
                     /translation="MNRRHFLGALAAATVAAVGAARLVVDPQPRTFAETPVGDVATSG
                     PTAPAALLPPPPLSARIPLPGGGALTTIPGEGDLLALTVDDGVNSEVVRAYTQFAKDT
                     GVRLTYFVNGVYKSWTENLAMLRPLVDSGQIQLGNHTWSHPDLTGLSKDEVAEQITRN
                     DEFLKNTYGIGAKPYWRPPYAKRNDDVDAVAADLGYTVPTLWSGSLSDSTLITEDYIV
                     QMADQYFTPQAIVIGHLNHLPVTHVYPQLVEIIRDRNLRTVTLNDVFLKTP"
     gene            7212..7979
                     /locus_tag="VFI55_10375"
     CDS             7212..7979
                     /locus_tag="VFI55_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET7074471.1"
                     /translation="MNAPDPQRLGGLAWTRRTGGRLSRTERRRLLIAIAVGQLQNAVG
                     RAKLALGRLPAGAARVDLDEFRAPDSKFAREAEEACAELPPTLQGHSYRTWLFGRALA
                     AIDGQRLDDELFYCGALLHDYGIVSPTARRDFTLGSVERMLACADVAGLDGARADLLA
                     DGICVHTTPGVEVGRDGAMGCYLQWGAMVDGAGLRMWDIAPRDVEMVLRRYPRGDFKR
                     ELIGMMRAEAAAVPKGRFGLLVRCGLPLAVRMAPFDS"
     gene            complement(8036..8293)
                     /locus_tag="VFI55_10380"
     CDS             complement(8036..8293)
                     /locus_tag="VFI55_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein L7/L12"
                     /protein_id="HET7074472.1"
                     /translation="MEDPLVYRRLALLEQQVRLLSERVGVPCPTFGIDVGTSAPTQLD
                     GGVPTEVVVLARAGRKIAAIKLYRELTGAGLREAKHVVDSL"
     gene            complement(8316..9704)
                     /locus_tag="VFI55_10385"
     CDS             complement(8316..9704)
                     /locus_tag="VFI55_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007530871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7074473.1"
                     /translation="MSLTTATDELRGRFGAEIFLPDDPGYDEARALHNAMIDKRPAVI
                     VRCASTTDVAGALEFARTSNLAVAVRGGGHNGPGFGSVEGGLVLDLSPMNRIDVDADR
                     RTARVQGGATWAQVDRATHAYGLATPAGIISTTGVGGLTLGGGHGYLSRKHGLTIDNL
                     LEAEVVLADGQVVTTSESEHPDLFWALRGGGGNFGVVTSFTFRVHPVHTVICGPTAWP
                     ASATADILSWYRDFLPAQDEDLYGFFASMTVPPVAPFPDAFHLQKACAVVWCYTGDPA
                     RADEVFAPVRQMQPAFEGLGAAPYPALNSTFDALYPKGLQWYWRGDFIRTVPDAAVAA
                     HARFTQELPTMHSTMHLYPIDGAVHGVGQTETAWAYRDVNFSQVIAGVDPDPANAEVL
                     KRWTREYWEAIHPYSAGGAYVNFMMDEGQERVRATYGPNYERLSEIKAQYDPGNVFHI
                     NQNILPAGSPGT"
     gene            9864..12773
                     /locus_tag="VFI55_10390"
     CDS             9864..12773
                     /locus_tag="VFI55_10390"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7074474.1"
                     /translation="MAHWVPPPLPAELMARRRGPLVGRDAELAAFELAWERVVGGSRQ
                     AVFIGGEPGAGKTRLAAEVAGTLAAHDVAVLVGRSTADEDIPYAPFAVALDRLLTAGP
                     SGSMADLLGAAGPQLRRLSTQVDRHLPDTEPAMDVGSPRQALFDALTGFLRRLAVDRP
                     IVLILDDLHWAQLPTLAMLEHVLIACADVRMLVVSTFRTTEPDRSDELTTRLAELHRF
                     EGVRRLDLAGLDTEAIAEYVRRTQQLAPASARTAAALLRDKTGGNPFFLTELVNELEI
                     RGGLAMLSVQQTVPASIGDAIARRLGGLGAAVRGVIEQAAVLGETFDLPALIAASETD
                     LPATLAAVDSAEAVGLVRPVRDSDGEFSFVHALTREAVVGGMAASRLRIMHARAAEAL
                     QSRADPSVVPRLANHFLRAHVLGYHEQALRYAHQAARMAEQSLAYEDAARWFERAASL
                     PELSRADRARLDLDAAANHVRAGDFTRSRAIYERLSAMDDPLIGLAAAVGYEEANWRR
                     GQSDSTAADLLASALESCGLNTDDPRYVQALGSFGRALAFAGEAARAREVGNNAIDCA
                     RAADDPAVLMHTLKTSLWHGLTPELCEIQAERSAEVSRMALARRDYEVLGASSHFRAM
                     VSYLAGRPDDLAASTADMRRAGQAIGQPVFGFVGACVEQALAYLRGDFAGAERWADIA
                     LRTGDLFDADDTEGSNGVQMFMIRRETSDLKKFAGFVDGTETFAGRWVPGLLALYTEL
                     NCEAGMTRALNHLLNRKLGDRTNEAQWPMELAFMVEAALELSNREALHGLRPYILRYA
                     GKNLVAGQFVALFGSADRYLARMAALSGDAAAAERHFGAALEMDRRMGSVVHVSETLA
                     RHALFAASRGRTEEARRLADEARAIAMQIGQNRVVDLVDALLTMSLEGPDGLTDREIE
                     VLRLLAEGLSNRAIGERLFISTNTAANHVRNILIKTGAANRTQAAMYAADHELLG"
     gene            complement(12779..13048)
                     /locus_tag="VFI55_10395"
     CDS             complement(12779..13048)
                     /locus_tag="VFI55_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HET7074475.1"
                     /translation="MPIFMVERTFAEELEPTLEIADGINRINDQEGVRWLYSFLSADK
                     RKTYCLYEAPSPEAIRTAAARAGLPADVVVEVRDRIMPDGALSDI"
     gene            complement(13104..13562)
                     /locus_tag="VFI55_10400"
     CDS             complement(13104..13562)
                     /locus_tag="VFI55_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET7074476.1"
                     /translation="MRRVLNGEWEPTVPALVNLVAAAAAPHLRAAFAAAGLDGIRPAQ
                     AVALVPLAAGGLHASDLADQLGVSRQAVAQGIAALERHGYVTRVPDPVDARAQIIELT
                     PRGRQALRVMRSNAVDLEKRWEEVLGAGRLDELREILQMLLAGETGAAGE"
     gene            13639..14574
                     /locus_tag="VFI55_10405"
     CDS             13639..14574
                     /locus_tag="VFI55_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HET7074477.1"
                     /translation="MRCARLLGVCLLPVLVVVLGGCLGGGHALGMPGAQSIPVGQSTQ
                     TVESGGVDRTFHLYRPQGLTDAVPLVVMLHGGFGSGEQAERSYHWDNEADGGHFLVAY
                     PDGLNRAWNAGTCCGEPQRVNADDVGFITAMVGAIEQEMPIDRARVYVTGMSNGAMMA
                     LRLGCQTDTFAAVAPVAGTLLTDCSQARPASVLQIHGTADDRVPYNGGPGKAFASNGN
                     PRVDGPPVESVNATWRAIDACGPPTTSTAGDVTTETAGCPDGRTVELISVAGAGHQWP
                     GGEPSPLAELAGIPAPSTALDATDTIWQFFAQSHR"
     gene            14624..15805
                     /locus_tag="VFI55_10410"
     CDS             14624..15805
                     /locus_tag="VFI55_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074478.1"
                     /translation="MLADVGMMAGFELVRFSGCALWACAAFTVMLCGSCAGPPHGGTS
                     SHQTPGKPLGHSGCGPPITYQVFQQGASGSNWSKANDRIAFNSKASDGRFHIYTVKPD
                     GTNRQQLGVGSATFPQRTTGTPAWSPSGRFVAFVAEKSNHPGNSVGATPGWGSYSDLW
                     VATADGSHAWQLTDVPTDKDHGTISPEFSPDGRLLEWTERTAAAKALDPNRFAGFWVI
                     KVADFVVSQDGTPSLSGVRTISPAGDAFNETGGFSADSTSLVFTSNFETHNFWKSQIY
                     RLDLNSDAIARLTRGDSYNEHPRYTPDGQVLWMTNADNPSHGTDWWTMDADGSHQQRL
                     SDFNSDGDARGFGSGRVWATTVQTDGWSPDERYFYGDVETNLVNSDGVILRASLTCGG
                     S"
     gene            complement(15809..16417)
                     /locus_tag="VFI55_10415"
     CDS             complement(15809..16417)
                     /locus_tag="VFI55_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7074479.1"
                     /translation="MEIKRRTQEERSAATRDALISAARKLWGLRGYAEVGTPEIATAA
                     GVTRGAMYHQFADKAALFSEVVEVVEQDVMARMATLVTKSGATSPADAIRAAVDAWLD
                     VSGDPEVRQLILLDAPSVLGWSAFRDVAQRYSLGMTEQLLTQAMAAGQLAHQPVRPLA
                     HVLIGALDEAAMAIATADDPKRAHRETGEVLHRLIDAMLSER"
     gene            16482..17312
                     /locus_tag="VFI55_10420"
     CDS             16482..17312
                     /locus_tag="VFI55_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7074480.1"
                     /translation="MPTIDINAGTIHYEATGPENGRPVVFVHGYMMGGQLWRRVSERL
                     GDQGLRCIAPTWPLGAHPEPLRHGADRSITGVAGIVAEVLDALDLEDVVLVGNDTGGV
                     VTQLVAVHHPDRLGALVLTSCDAFEHFPPPILKPVIVAAKSKALFRTAIQAMRAPVAR
                     KRAFDGLAHRNIDDLTEVWVRPVLSDPAIAEDLRQFTLSMRTEVTTGVAARLPEFDKP
                     ALIAWSADDVFFEAADGERLAATIPNARLEVIEGARTFSMLDQPERLADLLSTIAVRA
                     "
     gene            17409..19031
                     /gene="fadD1"
                     /locus_tag="VFI55_10425"
     CDS             17409..19031
                     /gene="fadD1"
                     /locus_tag="VFI55_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid--CoA ligase FadD1"
                     /protein_id="HET7074481.1"
                     /translation="MAGDTIQALLHERLSDSSVAVKHGNSQWTWRQYLTESTTRAAAL
                     IAAADPQRPMHIGALLGNTTEMLIQMAAAGLGGYVLCGLNTTRRGEALAADVRRADCQ
                     FVVTDAGHRPLLEGLHLGETQILDTSTPRWAEFVGGAGELVPHRHVTAMDPFMMIFTS
                     GTSGNPKAVQVSHLMATIAGLSLTERFSLTERDTCYVSMPLFHSNAVVAGWAPAVASG
                     AAVVPAEFSARNFLDDIRRYGATYMNYVGKPLAYILATTERDDDADNPLRVAFGNEAN
                     DKDIEEFARRFDVHVEDGFGSTENAVIVIREPGTPRGSIGRGVDGIAIYDSETVTECA
                     VARFDANGALANTDEAVGELVNTAGSGFFTGYYNDPEANAERMRHGMYWSGDLAYRDS
                     EGWIYLAGRTADWMRVDGENLAAAPIERILLRHKAINRVAVYAVPDERVGDQVMAAIV
                     LSAGRTLDPDAFEAFLDAQPDLSPKARPRYVRLSADLPSTATHKVLKRHLIAQGTAIG
                     AGEVLWEREPRGTSYRHAAPGPSIGSVSQEAD"
     gene            19092..20102
                     /locus_tag="VFI55_10430"
     CDS             19092..20102
                     /locus_tag="VFI55_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7074482.1"
                     /translation="MGHAKARTVVQLSTNKTVRPTTADVARLASVSTATVSYVLNNAE
                     GRRISAQTREAVQRAAELLGYRPNLAARNLARGKSGVVLYVVPHVAVGEMPMQAGSRM
                     TTELARSGLLQVQIFETEDDRHIVDAIEVLDPIAVTSLFPLSGPAFDAVRTAGIPHIE
                     IGTLPALGDPHLSVGEMRVGHLVARGHTLIAFAYTGIAKWRALGDYWFEGVSRAARLR
                     DLPPVRVGDITPANAADVVTGWVRDGVTAVCAQSDEIACLVLYGVHEARLRCPDDLAV
                     IGVDASPVGAVSTPPLTSVEFDPRAVADVAVDAIFERLGLARPPAGAPTEIARLIVRS
                     ST"
     gene            20213..21667
                     /locus_tag="VFI55_10435"
     CDS             20213..21667
                     /locus_tag="VFI55_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7074483.1"
                     /translation="MSVQAVLDEIRDRPGTGETIAVFDPATEEQVTEFKDCGTEAVDD
                     AVARAKASFESGVWRDKPPSERAKILWRVGELIDQNAELLADLESLNAGMTPLQAQGT
                     VTVGSEFFRYYAGWCTKIEGIAADVHTGGLTGIDSHQHVYTLKEPYGVVGLIFPWNGP
                     VFNFCAKLAPSLAAGCSSVVKPAEETPLSALVLDNILREAGVPEGVANLLVGYGHTAG
                     AAITAHPDVEKVAFTGSTEVGRAIVHAAGESNLKKVTLELGGKSPVVVFDDADLSKAV
                     PMAAFGTFIHSGQACVCGSRIFVQRGVYQQFVESLATVADGLPQGGPKDEGSLIGPLI
                     SQKQLTRVLGYLDQGKADGVEVVTGGYRLDRKGYFVHPTVLTNVNSTSRLFQEEIFGP
                     VVAVLPFDDEDEAVALANDSTYGLAATAWTKDLGRAHRIAKRLKAGTVGLNCQMQFDH
                     SMPFGGYKQSGWGYESGKAGLDTYLQTKIVWAQM"
     gene            complement(21746..22639)
                     /locus_tag="VFI55_10440"
     CDS             complement(21746..22639)
                     /locus_tag="VFI55_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HET7074484.1"
                     /translation="MPNQQQADRMTSGKGFVAALDQSGGSTPKALRLYGIKDDAYSSE
                     EEMFDLIHQMRARIITSPAFTGDRVLAAILFEQTMDREIEGQPSTTYLWETKGVVPIL
                     KIDKGLAEASDDVQLMKPMPGLDDLLQRAVAKGVFGTKERSVIGGANTAGVAAVVAQQ
                     FEVAQQVLSHGLVPILEPEVTISIPDKAAAEDILRDEITKQLETVPDGQRVMLKLSLP
                     SEVNHYRAFVEHPKVMRVLALSGGYSREEADELLAKNTGVIASFSRALTEGLSADQSD
                     EQFNATLDKAIQSIYEASVAG"
     gene            complement(22745..23737)
                     /locus_tag="VFI55_10445"
     CDS             complement(22745..23737)
                     /locus_tag="VFI55_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7074485.1"
                     /translation="MADLSIQDLVVEYSSGGYALRPINGLNLDVAAGSLVILLGPSGC
                     GKTTLLSCLGGILRPKSGAIKFGGIDVTALDGRELAKYRRDTVGIVFQAFNLVPSLSA
                     LENVMVPLRAAGWSRAASRRRAEELLTRVNLADRMKHRPGDLSGGQQQRVAVARAIAL
                     EPPLILADEPTAHLDFIQVEEVLRLIRSLAEGERVVVVATHDSRILPMADHVVELAPA
                     HASSNRLPETVQLKAGEVLFEQCTMGDLIYVVSDGEFDIVRELADGGEELVKVAGPGD
                     YFGEMGVLFHMPRSATVRARTDAAAVGYTAQAFRERLGVTGISNVIAQPEVARG"
     gene            complement(23741..24790)
                     /locus_tag="VFI55_10450"
     CDS             complement(23741..24790)
                     /locus_tag="VFI55_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7074486.1"
                     /translation="MLLAALRDMQWRRRRLVIAIVSTGLVFAMTLVLTGLANGFRVEA
                     QRTVDSMGVDAFVVKAGAAGPFLGSTPFAQVDMARVAAEPGVVAAAPLATAPATITEG
                     TSSRNATAFGAPAHGPGMPQVSEGQVPTLPGEVAVSSTLGKHVGEELQVGARKLQIVG
                     IVPNATALAKAPCLFLTTEGLQQLSYNGQPMITSIGIIGTPRQLPDGYQAVDRTAGVN
                     DLLRPLKVAVGAISIVALLLWIVAVLIVGSLVYLSALERLRDFAVFKAIGVPTRSILA
                     GLALQAVVVALLAAVLGVLLAQLLSPVFPMIVVVPVRAYFALPGIAIAIGLLASVAGL
                     RRAVAVDPALAFGGP"
     gene            complement(24903..25496)
                     /locus_tag="VFI55_10455"
     CDS             complement(24903..25496)
                     /locus_tag="VFI55_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HET7074487.1"
                     /translation="MTGKANETATAPTRFALPSRILHWLMAPMVIAQLLIGVTMIASL
                     TYYPLLLAIHRPLGILILAFAVVRLVNRLTHRLPPFLATMSPAERRVASWSEYLLYAL
                     LLVQPLIGWAMLSAARFPVILMGPLALPGIAPHDIDVYAALRQAHTVCAFLLFAAFTA
                     HVCAVLFHTLVLRDRLLDRMALWPTKTIAPQQNPTQP"
     gene            complement(25493..26650)
                     /locus_tag="VFI55_10460"
     CDS             complement(25493..26650)
                     /locus_tag="VFI55_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HET7074488.1"
                     /translation="MEPGDHGGRSHEPDDESDKPIETKPTDSSWRTALTRRTALLGMG
                     AVAGFAAVDVGGFAYAGGWLRPDAPASLTPSRFADRFEDVYGHHDGFRRNHAKGLSAT
                     GSFASSGAGAAICRAAVFRPGSVPLVGRFSLGGGLPDQADKPETVRGLGLVFQPPDGA
                     QWRTAMINFPVFTDSTPQGFYERLLASKPLPDTGKPDPQKMAAFLDRHPETVAAMKII
                     KQSPPSAGFADSTFYGLNAFWFTNSAGASVPVRWSVVPQDAGGPPAPSRGKDFLFDDL
                     IRAVAQRPLRWRLVLTVGEPGDPTNDATTPWPQSRRSIDAGTVTINAVQTEEAGNGRD
                     INFDPLVLPDGITASDDPLPAARSAVYARSFTRRAEEPKSASEVNVARVLS"
     gene            complement(26822..27814)
                     /locus_tag="VFI55_10465"
     CDS             complement(26822..27814)
                     /locus_tag="VFI55_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7074489.1"
                     /translation="MGDLSIQDLVVEYDSGGYALRPINGLNLDVTAGSLVILLGPSGC
                     GKTTLLSCLGGILRPKSGAIKFDDVDITTLEGAALANYRRDKVGIVFQAFNLVPSLTA
                     LENVMVPMREAGMSRRAARTRAKELLTRVNLEERMKHRPGDLSGGQQQRVAIARAVAL
                     DPPLVLADEPTAHLDFIQVEEVLRLIRELADGDRVVVVATHDARMLPMADRVVELTPD
                     FPETNKPPETVELKAGEVLFEQSTMGELIYVVSKGEFELVHELADGGEELIKVAGPGD
                     YFGEIGVLFHMPRSATARARTDATAVGFTAPAFRERLGAGGVRDLIEHRKLAGN"
     gene            complement(27817..28866)
                     /locus_tag="VFI55_10470"
     CDS             complement(27817..28866)
                     /locus_tag="VFI55_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7074490.1"
                     /translation="MLFAALRDMQWRKRRLIIAIVSTALIFGMTLVMTGLANGFGVEA
                     RNTVDSMGVDAFVVKDGAAGPFLGSTPFPEVDLARIAAEPGVVAAAPLACVGTIMKQG
                     TSTRNVTAFGAPEHGPGMPHVSQGQAPSKPDEVAASSTLDRHIGDTLQVGAHVLRIVG
                     IVPNSTALAKIPNIFLTTQGLQQVAYNGQPMVTSIGVVGKPRHLPDGYQSYDRTGAVK
                     DLVRPLKVAVTSISIVAVLLWVVAALIVGSVVYLSALERVRDFAVFKAIGTRTRSIMA
                     GLAMQALVVALLAAIVGVILAQLLAPLFPMIVAVPAGAYLLLPVVAIVIGLLASLAGL
                     KRVVAVDPALAFGGP"
     gene            29156..29548
                     /locus_tag="VFI55_10475"
     CDS             29156..29548
                     /locus_tag="VFI55_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7074491.1"
                     /translation="MVEQSGDSVDPTSFEVGKHQVDQAVVVTVSGEVDMLSAPRLAEA
                     ISTALAAAPAALIVDLSKVDFLASAGMTVLVTAQAQVVPPTRFAVVADGPATSRPLKL
                     MGLDTVLSLYSALDSALSGVDGGRTSAE"
     gene            29555..29995
                     /locus_tag="VFI55_10480"
     CDS             29555..29995
                     /locus_tag="VFI55_10480"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7074492.1"
                     /translation="MSYIDDRSRFIRTRVAADARSAARTRAEFGRWLDRHFTLGAQRF
                     SDLLLAVYEAIANAAEFAYVDSSLRRTMDVSAAYDVESDTLAVTVDDRGRWRQSRPEP
                     ATVPRRTQLRGRGIQLMRALADQVTIDRTPQGTHVTLTWIELTR"
     gene            30150..>31856
                     /locus_tag="VFI55_10485"
     CDS             30150..>31856
                     /locus_tag="VFI55_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7074493.1"
                     /translation="MFDLKITGGTVVDGTGGQRYRADIGIKDGVIVDVVRRDAGDAEP
                     SGLETALEMADAAETIDATGHVVAPGFVDIHTHYDGQVSWDSLLEPSSGHGVTTIVTG
                     NCGVGFAPVRPGSEQWLIELMEGVEDIPGTALTEGITWGWQTYPEYLDAIGKLKFAID
                     VGSQVAHGAVRAYAMGERGARNEPATPEDIAAMGRLVTEAIEAGALGFSTSRTLGHRA
                     MDGEPVPGTFAAEEELFGLGRAMAAGGQAVFELAPQGSAGEDIVAPKKELDWMRRLGG
                     EIDRPVSFALIQVDADPKLWREMLDLSADAHAAGSRLHPQVAARPFGMMIGFQGHHGF
                     SHRPTYRGLAAECSREELAQRLADPAVKAAILSEDDLPVDPTLLFDGMFALVQHSLKR
                     LYALGDPPDYEPTPDRTVAAIAKARGEDPLSTLYDLMLERNATAMLMLPLFNYADGNH
                     DAIREMMLHPAGVLGLSDGGAHCGMICDASYPTFLLTHWARDRHRGEKLSLEYVIRKQ
                     SRDTAHLFGLTDRGTIEAGKKADINVIDMDALRLHPAAMAFDLPAGGHRILQRASGYA
                     ATI"
BASE COUNT         5426 a        10726 c        10535 g         5169 t
ORIGIN      
        1 tgctcacctg ttggggcccc gcgtcgacga cgctcacagt cacgacggtc tcctctcgga
       61 aatccggcag cgtacggtcg acgctacgcc tcggcaagtt gcacttctac cactgtgctg
      121 acacgtgctc gaacgctcca acccgctcga caagatcgcg cgcgactagc tggaaaggaa
      181 ccgaacggac cgatgagcca gtcaatctcg ggcaaggtgg cgttcatcac cggcgccgcc
      241 cggggacagg gccgggcgca cgcggtgcgg ctgtccgccg aaggcgccga catcatcgcg
      301 ctcgatatcg ccgggcccct gccctccagc gttccctacg actcgccgac acccgacgac
      361 ctggccgaaa cgggacggct ggtgcgggcc aatggcagga aggtcgtcac ggcggcggtc
      421 gacatccgcg acttggacgc gctcaaggcc gccgtcgaca acgcggtcgc cgaactgggc
      481 cgtctcgaca tcatcgtcgc caacgctgga atctgcagtc ccgcgccgtg ggatcagatc
      541 accccggaag cgttccgaga caccatcgac accaacgtgg tcggcacctg gaacacggtg
      601 atggccggtg cgcagcacat gatcgacggc ggccgcggcg ggtcgatcat tctcatcgga
      661 tcggccgcgg gcatcaacat gcagtcgttc atgatccact acacggccag caaacacgcc
      721 gtcgtcggaa tggcgcgcgc gttcgccgcg gaactgggcc ggcacaacat ccgcgtcaac
      781 agcctgaacc cgggagcggt cgccaccccc atgggcaccg gccggatgcg tgaagccctg
      841 cattcggccg ccgacagtta cccgcatctg cgcggtatgc acaaaccgct gctgcccgaa
      901 gggattgccc agcccgaaga catcgccgac gccgtcgcgt ggctggcctc cgatcaatcc
      961 cgattggtta ccgcgactca gctttccgtc gatatcggcg tcggttacgt ctgacgcaac
     1021 ggcctcgggc gcgcgggtag accgccgacc gggcagaatc gtgctgcccc cggaccggcg
     1081 taacggccct tgacggaaat acccccgggg tgtatacgtt gcgtagacat gaataccccc
     1141 agtgggtata tgcggagagg agcgccgtga ccacgagcga ataccgggtg agtgggatga
     1201 gctgccgcca ctgcgaggcc gccatcgaga acgaggtcgg ccagatcccg ggtgtgcacg
     1261 gcgtcgacgt gagcgcgcgc acgggcaggc tcgttgtcac aagttcggcg cacatcgacg
     1321 cgaaggcggt gctggacgcg gtcgacgagg ccggctacgc ggcggtcccg gtcgcatgaa
     1381 agcacccgac actaccggct cacccaccac cgcgggaagc atcgagctgg cgatcaccgg
     1441 gatgacgtgc gcctcgtgcg cgatgcgcat cgagaagaaa ttgaacaagt tcgacggggt
     1501 cgacgcagcg gtgaattacg cgaccgaaaa ggccaccatc actatcccgc ccggtttcga
     1561 ccctctgacg ctgattgccg aagtcgagaa ggccggctac gccgcggcac tgccgcaaca
     1621 ccgcaggcga gggccggaga cgccgcaggt gaccgacgac gccgagctga cgtcgctgcg
     1681 cgcccggctg gttgccgcga tcgtgctcgc cggcccggtg atcgccatgg cgatgatgcc
     1741 cgccctgcaa ttccggtact ggcagtgggt gtcgctgggc ctggccaccc ccgtcatcgt
     1801 gtgggcgggg cggcccttcc acaccgccgc atgggtcaac ctcaaacacg gcgccgcgac
     1861 catggacacc ctcgtatcgg tggggacgct ctcggcgttc ctgtggtcgc tgtatgcctt
     1921 gttcttcggg acggccggcc aacccggcat gcgccacggc ttcgacctga ccgtcgcgcg
     1981 cagcgacggc gcgggcaaca tctacttcga agtggccgcc ggggtgaccc tgttcgtcct
     2041 tgccggccgc tacttcgaga agcgatccaa gcgacgcgcc ggagccgcct tgcgcgccct
     2101 gctgcagctc ggcgccaaag acgtcgcggt gctgcgggac ggcaccgaga cccgcattcc
     2161 cgtcgagcaa ctcgcggtgg gcgacgagtt cgtggtgcgg cccggcgaaa agatcgccac
     2221 cgacgggacg gtcgtctcgg gctcgtcggc cgtggacgcc tcgatgctca ccggcgagtc
     2281 ggtgccggtc gaggtgggtc agggggacac cgtcgcgggc gccaccgtga acgccggggg
     2341 acgcctcgtc gtgcgagcca cccgggtcgg cgacgacacc cagttggcgc agatggcaaa
     2401 gctcgtcgaa gcggcccaat caggcaaagc cgacgttcag cggctggcgg accgcatctc
     2461 cggcgttttt gtgcctgttg tcatcgctgt cgccgtggcc accctcgggg tgtggctcgt
     2521 cgccggctcc tcggtcaccg cggcactgac ggccgccgtc gccgtgctca tcatcgcctg
     2581 cccgtgcgcg ctcgggctgg cgacgccgac cgcgctgctg gtcgccaccg ggcgcgcggc
     2641 tcagctcggc atcctcatca agggacccga agtgctcgag tccacccgca gggtcgacac
     2701 gatcgtgctc gacaagaccg gcaccgtcac caccggccgg atgaccctgg tggacgtgat
     2761 tacctcgccg ggcaccgacc gcgaaacgct gctgcgctac gccggcgccg tggaggacgc
     2821 ctcggagcat cccgtcgcac aggcgatcgc ccggggcgca aaggccgaac tgggcaccct
     2881 gcccggcgtc gagggcttca cggcagtggc gggcaacggc gtgcacggca ccgtcgacgg
     2941 tcacgtcgtt gtggtcggac gcgagagcct gctggccgat tgggcccaac acctcgattc
     3001 cgacttgtcg gcggccaaag ccggggccga gggcgagggc aaaaccgcag tcgtcgtcgg
     3061 ctgggacggt cgcgcccacg ggatcttgat gatcgccgac accgtcaagc ccaccagcgc
     3121 ccaggcaatc cggcagttca aggccctggg actgacgccg gtgctgttga ccggcgacaa
     3181 cgccaccgtc gccgcgcgca ttgccgacga ggtcggcatc acccacgtca tcgccgaggc
     3241 gatgcccgcc gacaaggtcg ccgccgtcca gcgtttgcag gcggagggca aggtcgttgc
     3301 catggtcggc gatggggtca acgacgcggc ggcactggcc accgccgatc tgggccttgc
     3361 catgggcacc ggtaccgacg tggcgatcga agccgccgac ctgagcctgg tgcgcgggga
     3421 cctgggcgcc gcggcggacg cgatccggct ctcccggcgg acgctgcgca ccatcaaaac
     3481 gaacctgttt tgggccttcg gctacaacgt cgcggcaatt ccgttggccg cgcttggcct
     3541 gctcaacccg atgctggcag gggccgcgat ggtgttctcc agtgtgttcg tcgtcggcaa
     3601 tagtttgcga ttgcgctctg tcacaaccac gatgcgtgcg gatcctgtgg gatagttgtt
     3661 ctggcctacc gcgttccaat gccgcgaccg gtgggtggtt cattcaggct gctttgcaga
     3721 acgatcggat ttttgaaggt cacgaccgtt gcccaacttc gtcgaacagc tgtccttgct
     3781 gcatggctgg ctacgaccga cggcgcaagc gctggcgctg gtcgtggccg ccggcgcgat
     3841 cggatggcgc cggcgacgtt ggcgtagctg ggccctcgtg gccgcgttgg gcgtagcggc
     3901 gctggtcacg gcgctgtcgt attggtacat cacatcgctg ggcgtggccg gggacccggc
     3961 accggcgtcg ttgtggctct ggatagcgat gagcggactc gcgatcgccg tcctgttgct
     4021 cggatggccg ggtgcccgct ggtggcgacg cgctatggcg gtacttttgg tgcccctgtg
     4081 cgcgctgtgc gcggcgttgg ccttgaacgg ttgggtcggc tatttcccca cggtgctggc
     4141 cgcgtggaat cagctgacgt cgggaccctt gcccgaccag atcgatcggc tgcgggtcac
     4201 cgaaatgcaa gtcgccggga ccaggcccgc gaagggcgtc gtagtgccgg tgaacatcaa
     4261 ttccaacgcg tcgcactttc cccaccgccc ggagctcgtg tacctgcctc cggcgtggtt
     4321 cagcagcaat ccgccgcctc gcttgccgac ggtgatgatg atcggttcgg cgttcaatac
     4381 acccgctgac tggctgcgcg ccggcggcgc tttcgacacc atcgataact tcgcggccca
     4441 tcatcagggt ttcgccccgg cgctggtgtt cgtcgatgcc actggcgcct ttgacaatga
     4501 cactgagtgt gtcaatggca gccgcgggaa cgccgcggat cacctgacga gggacgtggt
     4561 gccgttcgtc gtgtcaaatt tcggggtgag cgccgaccgg gcgaattggg gcctcgccgg
     4621 atggtcgatg ggtggtacgt gcgccgttga cctggccgtc acgcatccgg acatgttcag
     4681 cgcgttcgtg gacatcgccg gcgaccgcag ccccaatgtc ggcaccaagg agcaaaccct
     4741 cgcccggttg ttcggcggca acaccgccgc gtgggccgct ttcgacccgg tcaccgtcat
     4801 gaaccggcac gggcactaca ccggcgtgtc gggctggttc gacgtccccg cgtcgtcggg
     4861 cccgcgcaat ctcgcccagg aagccagtcc cggcctcacc acgtccagca ccgatcccgc
     4921 cgccaacccg gaaggccagg acgccgcggc taacacgctg tgcaccgcgg ccacagccaa
     4981 cgggatcacc tgcgcggtgg ttactcaacc gggcaagcac gactggccgt tcgccgccca
     5041 ggcgtttgcc gcctcgctgc cgtggctggc ctcgacgctg gggaccccga gcgtcgagcc
     5101 cgtccggtta cctcagcgcg ccggcggggc gcctcattga tccaccaccg ctggtgcgct
     5161 ccatctagtg gcggtgcggc tcgagcaagc gggcctggcc ggtcgaggga gggcacgctg
     5221 ccagccactc tcgcgtagcc gcgtgcgatt gctcgatcag ggttgccgcg tgcgagaagt
     5281 ccgctcctga tgtcgcgatc ggacacagcg gtgggacgac gtgcagctcg accgcctgct
     5341 cgaagcgggc tacgtcgacc gccaggcgat ggttgatggt cagggtcagg gcgtgcaacg
     5401 ccatcgtcag cgcagactgc ggtaaggcgg gcaggtcgca cgagtagccg gtgggcaaca
     5461 cccacacccg gtcggcgccg agggcgacgg catgggacag tggcgtgttg ttcacgaccc
     5521 cgccgtccat cagaccgcgg ccgttgatcg tcaccggtgg gaagatgccg gggatggcgg
     5581 cgctggccgc gacggcgtcg accgcgtctc ccgacgacag cagcacatcg gtacccgaga
     5641 tcacgtcggt ggccaccaca tggaacggga tcggtgcatc ctcgagccga gtgaacccca
     5701 gctgctcgga cagtagccgt cgcagaccac tgctgggtat caggtgcgga cgacgaccca
     5761 ggaagcccat cagcccgccg accagatgag tgggaaatat ctcttttcgc gacagcgaca
     5821 gccacaagtc ggccagtgcg tcaatgccgg cggcgtcggg ccgggatgcg acccaggcac
     5881 cgttgagcgc gccgacggag gtgccgacga tcaggtctgg cgttatgcct tcaccgacca
     5941 gggcacgcaa catccccacc tggaccgcac ccaggctggc acccccggac aatacgaatg
     6001 ccgtggtcat gtcatcacac aaccaccggc gcggcatggc agccgagcac gaatgtcgcc
     6061 gttgtgaccc cgttgtcggc gacggtcatc ggggcgggca catgcgaata gcgggaacca
     6121 tcgctactgc tcctggcggc ccttgacgcc gccgtaccgt tgctgtttcg atatcgaatt
     6181 cacagctgac cctcccgtca cgagtccggc atattgccag tactaatggt acggttcgct
     6241 gctgtggctg tggagtctgg caacacccgt atcccccgtg cggggacgcg tgtgccgaca
     6301 tcgcaccgca aaagacccgc gaaaaaccgc gtcaccttcg tcccgccgtt tgcgggtgct
     6361 tccggtcgca accccgcccc gtcggagctg aaccgacgcc atttcctcgg cgcgctggcc
     6421 gcggccactg tcgccgccgt gggcgcggcc cggctggtgg tcgatccgca accacggaca
     6481 ttcgctgaga cacccgtcgg cgacgtggcg acgtccgggc caaccgcacc ggccgcctta
     6541 ttaccgcccc caccgctgag cgcacgcatt ccactgcccg gcggtggtgc attgaccacg
     6601 attcccggtg aaggcgattt gctggcgctg accgtcgacg acggcgtcaa cagcgaagtt
     6661 gtgcgcgcct acacgcagtt cgcgaaagac accggggtgc ggctcacgta cttcgtcaac
     6721 ggcgtttaca agtcctggac cgagaacctg gcgatgcttc gaccgctggt cgactccggg
     6781 cagatccaat tgggcaacca cacctggtca caccccgact tgaccggcct gtcgaaagac
     6841 gaggtcgccg aacagatcac gcgcaatgac gaattcctga agaacaccta cggcattggc
     6901 gcgaagccgt actggcggcc gccgtacgca aagcgcaacg acgacgttga cgccgtggcc
     6961 gccgacctcg ggtacaccgt cccgacgtta tggtcggggt cgttgtcgga ttccaccctg
     7021 atcaccgagg actacatcgt tcagatggcc gatcagtact tcaccccgca agccatcgtc
     7081 atcgggcacc tgaatcattt gcccgtcaca cacgtctacc cgcaactcgt cgagatcatc
     7141 cgcgaccgca accttcgcac cgtcaccctc aacgacgtct tcctcaaaac tccctagcgg
     7201 acgccgcgcc aatgaacgcg cccgatccgc agcggctcgg cggtctcgct tggacccgtc
     7261 gtaccggcgg tcgactgtcg cgcactgaac gccgccggct cctcattgcc attgcggtgg
     7321 gccagttgca gaacgccgtg ggcagggcca agctggcgct gggccgcctt ccggcaggcg
     7381 ctgcgcgcgt tgacttggac gaattccggg cacccgattc gaagttcgcc cgcgaagccg
     7441 aagaggcgtg cgcggagttg ccgcccaccc tgcagggtca ctcctaccgc acgtggctat
     7501 tcgggcgtgc gcttgcggcc atcgacgggc agagactgga cgacgagctt ttctactgcg
     7561 gagcgctgct gcacgactac ggcatcgtca gcccaaccgc gagacgcgat ttcacactcg
     7621 gaagcgtcga acgcatgctg gcttgcgccg acgtagccgg acttgacggt gcacgtgcgg
     7681 atctactcgc agacggcatc tgcgtccaca cgacaccggg agtcgaggtc ggccgcgacg
     7741 gcgcgatggg ctgctacttg caatgggggg cgatggtcga tggcgccggg ctgcgcatgt
     7801 gggacatcgc tccccgcgat gtcgaaatgg tgttacgccg ttacccgaga ggcgatttca
     7861 aaagggaact cattggcatg atgcgcgcgg aggcggcggc agtaccgaaa gggcgtttcg
     7921 ggctgctcgt tcgttgtgga ttgccgctcg ccgtgcggat ggcgccattc gactcctgag
     7981 gagaccgaat gacgttgcgg tgcaagcgga taacatcgcg ctgaatggat cttgcctaca
     8041 gcgagtcgac gacgtgttta gcctcgcgca atcccgcgcc ggtcagctcg cggtagagct
     8101 tgatcgcggc gatcttcctt ccggcacgcg caaggacgac aacctcggtg ggcacgccgc
     8161 catcaagttg tgtcggtgcg ctggtgccta cgtcgatgcc gaacgtcggg cacggtaccc
     8221 caaccctttc cgacaacagg cgcacctgct gttcgagcag cgcgagacgg cggtagacga
     8281 gcggatcttc catcccatca cgatatgcga gtcagtcagg tgccgggcga tccggcgggc
     8341 aggatgttct ggttgatgtg aaacacgttg cccggatcgt attgcgcctt gatctcgctg
     8401 agccgttcgt aattcggacc ataggtcgcg cggactcgct cttggccctc gtccatcatg
     8461 aagttgacgt aagcgccgcc ggccgagtag gggtggatag cttcccagta ctccctcgtc
     8521 cagcgcttca acacttccgc gttcgccggg tcgggatcga ccccggcgat gacctgtgag
     8581 aagttgacgt cccggtacgc ccaagcggtt tcggtttgcc cgacgccatg gaccgcgccg
     8641 tcgatgggat agaggtgcat ggtggaatgc atcgtcggca gctcctgggt gaagcgggcg
     8701 tgggccgcaa cggcggcgtc aggcacggtc cggatgaagt cgccacgcca ataccattgc
     8761 agccccttcg gatacagtgc atcgaaggtc gaattcagcg cgggatacgg ggccgcacca
     8821 agcccctcga aggccggctg catctgccgg accggcgcga acacctcgtc ggcccgagcg
     8881 ggatcaccgg tgtagcacca gaccacggca cacgcctttt gcagatggaa cgcgtcgggg
     8941 aacggcgcca ccgggggaac ggtcatgctt gcgaagaagc cgtagagatc ctcgtcttga
     9001 gcgggcagga agtcccggta ccagctcagg atgtcggcgg tggccgatgc gggccacgcc
     9061 gtgggaccgc agatcacggt gtgcaccggg tgcacgcgga atgtgaaaga cgtgaccacg
     9121 ccgaagtttc cgccgccgcc gcgcagcgcc cagaacaggt cgggatgctc ggattcggac
     9181 gtcgtcacca cctggccgtc ggccagcacg acctcggcct ccaataggtt gtcgatggtc
     9241 aggccgtgct tgcgggacag gtagccgtgg ccgccgccca gggtgaggcc gccgactccg
     9301 gtggtggaga tgatgcccgc cggcgtcgcc agaccgtacg cgtgggtggc gcggtccact
     9361 tgtgcccagg tcgcgccgcc ttgaacccgg gcggtgcgcc ggtcggcgtc cacgtcgata
     9421 cggttcatcg gcgacaggtc gaggacgaga ccaccttcaa cggaaccgaa tccggggccg
     9481 ttgtgaccgc cgccgcgaac ggcgaccgcc aggttcgacg tccgcgcgaa ttccagagcg
     9541 ccggcgacgt cggtggtcga tgcgcagcgc acgatcaccg ccggccgctt gtcgatcatg
     9601 gcgttgtgga gcgcccgggc ctcgtcgtat ccagggtcgt cgggcaggaa tatctctgct
     9661 ccgaatcggc cgcgcaattc gtcggttgcg gttgtcaggc tcatggccgc acacctcctg
     9721 tagatacttc gccggtcttg ctgccacgac gctacggggc ggcgaggtcg ctggtcatga
     9781 ccacaacgaa gcatctccga ggcattccac gatagtcaga actgactatc gaccggccag
     9841 accggcgcgg ttacgttgat tccatggcgc actgggtgcc gccaccgctt ccggcagagc
     9901 tgatggcacg ccgccgtggt ccgctcgtgg gtcgtgacgc ggaactcgcg gcgttcgagc
     9961 tggcctggga gcgggtcgtg ggcggcagcc gccaggcggt gttcatcggc ggtgaacccg
    10021 gcgccggtaa aacgcgcctg gccgccgagg tggccggcac gctggccgcc cacgacgttg
    10081 ctgtgctggt cgggcgctca acggccgacg aggacatccc ctacgcgccg ttcgccgtgg
    10141 cgctggaccg cctgctgacc gccggcccgt ccggttccat ggccgacctg ctgggcgcgg
    10201 cggggccgca gctgcgacga ctgtccactc aggtggatcg gcatttgccc gataccgagc
    10261 cggcgatgga cgtcgggtcg cctcgacagg cgctcttcga cgccctgacc ggattcctgc
    10321 gccgcctggc cgtcgaccgc ccgatcgtgc tgatcctcga cgacctgcat tgggcgcagc
    10381 tgcccaccct cgcgatgctc gaacacgtgc tcatcgcgtg cgccgatgtc cgcatgctgg
    10441 tggtgtccac attccgcacc accgaacccg accgctccga cgagctcacc acccgcctgg
    10501 ccgaactgca ccggttcgag ggcgtccgac ggcttgatct cgcgggtttg gacaccgagg
    10561 cgatcgcgga atacgtccgc cgaacccagc agttggcgcc cgcatcggcg cgcaccgccg
    10621 cggccttgct gcgggacaag accggcggca acccgttctt cctgaccgaa ctggtcaacg
    10681 agctggaaat ccggggcggt ctcgccatgt tgagcgtgca gcagaccgtc ccggcatcga
    10741 tcggggatgc cattgcccgc cgccttggcg gactgggggc cgccgtccgg ggcgtcatcg
    10801 agcaggcggc ggtgctgggc gagacgtttg acctgcccgc gctcatcgcc gccagcgaga
    10861 ccgatctccc ggcgacgctg gcggcggtcg attcggcgga agccgtcggg ctggtccggc
    10921 ccgtgcgcga ctccgacggc gagttctcgt tcgttcacgc gctcactcgt gaggcggtgg
    10981 tgggcgggat ggcggcgtcg cggctgcgga tcatgcacgc gcgggccgcc gaagcgctcc
    11041 agagccgcgc ggacccatcg gttgttcccc gcctggcgaa ccacttcctg cgggcgcacg
    11101 tgctgggcta ccacgagcag gcgctgcggt acgcgcacca ggcggcccgg atggccgaac
    11161 agagcctggc ctatgaggac gcggcgagat ggttcgagcg ggcggcctcg ctgcccgagc
    11221 tgagccgcgc cgaccgggca cggctggacc tcgatgcggc cgcaaatcat gtgcgcgccg
    11281 gtgatttcac ccggtcgcgg gcgatctacg aacgcctcag cgcgatggac gacccactga
    11341 tcggtctggc cgcggcggtt ggctacgagg aagcgaattg gcgtcgcggg caatccgact
    11401 cgacggcggc cgacctgctg gcgtcggcgc tggaatcctg cggactgaat actgacgacc
    11461 cgcgctacgt gcaggctctc ggcagtttcg ggcgagcgct ggccttcgcc ggcgaggcgg
    11521 cccgtgcgcg cgaagtcggc aacaacgcca tcgactgcgc gcgggccgcc gacgatccgg
    11581 cggtgctgat gcacacactc aagacgagcc tgtggcacgg gctcaccccg gaactgtgcg
    11641 aaatccaggc ggagcgttca gccgaggtct cacgcatggc gctggcacgc cgtgactacg
    11701 aggtgctggg ggcgtcctcg cactttcgcg ccatggtcag ctatctggcc ggccggcccg
    11761 acgatttggc cgcgtcgacc gcggacatga ggcgagcggg ccaagccatc ggacaacccg
    11821 tcttcggctt cgtcggcgcg tgcgtggagc aagcgctggc ctatctgcgc ggcgacttcg
    11881 cgggagccga acggtgggcc gacatcgcct tgcgcaccgg tgacctgttc gacgcggacg
    11941 acaccgaggg ctcgaacggg gtgcagatgt tcatgatccg gcgggaaact tcagacctga
    12001 agaaattcgc cgggttcgtc gacggcaccg agacattcgc cggacgctgg gtgcccggcc
    12061 tgctcgcgtt gtacaccgag ctcaactgcg aggccggcat gacccgcgcg ctcaaccatc
    12121 tgctcaaccg caaactcggt gatcgcacca acgaggcgca gtggccgatg gagctggcat
    12181 tcatggtcga agcggcgtta gagctgagca accgcgaagc gctgcacggg ctgcgaccgt
    12241 acatcttgcg ctacgccggc aaaaacctgg tcgccggcca gttcgtcgcc ctgttcggca
    12301 gcgccgaccg atatctcgcc cggatggcgg cactcagcgg ggatgccgcc gcggctgagc
    12361 gtcatttcgg cgcggcgctc gagatggacc gccgcatggg ttcggtggtt catgtcagcg
    12421 agacgctggc ccggcacgcg ttgtttgcgg cgtcgcgtgg gcgtaccgag gaggcccggc
    12481 gcctggccga cgaggcgcgc gcgatcgcca tgcagatcgg ccagaaccgg gtcgtcgacc
    12541 tggtcgatgc gttactgacc atgagtctcg agggtccgga cggtctgacc gaccgggaga
    12601 tcgaagtgct gcggctgctc gccgagggcc tgagcaatcg ggcgatcggt gaacgcctct
    12661 tcatcagcac caacacggcg gctaaccacg tgcgcaacat cttgatcaaa acgggcgccg
    12721 cgaaccgcac gcaggcggcg atgtacgccg ccgatcacga gttgctcggg tgagatcgtc
    12781 agatgtccga cagcgcaccg tcgggcatga tgcgatcacg cacttcgaca acgacatcgg
    12841 cagggaggcc cgctcgcgcg gccgcggttc tgatggcctc cggcgaggga gcctcgtaca
    12901 ggcagtaggt ctttcgcttg tccgcggaaa ggaatgagta caaccagcgg acgccctcct
    12961 ggtcgttgat ccggttgatg ccgtcggcga tctcgagggt gggctccaat tcctcggcaa
    13021 aggtgcgctc gaccatgaat atgggcacgg cggactcctg ttcattgtct tggcgacgaa
    13081 cctacgccgc caaccagcaa acgtcactca cccgccgccc cggtctcacc ggcaagcaac
    13141 atctgtaaga tctcgcgcaa ttcgtccaat cgtcctgccc cgaggacctc ttcccatcgc
    13201 ttctccaaat caactgcatt ggaacgcatt acgcgcagcg cttgccgacc gcgcggcgtc
    13261 agctcgatga tttgcgcgcg ggcgtcgaca gggtcgggca cccgagtcac atagccgtgt
    13321 cgctccaagg ccgctatccc ctgggccacc gcctgccggc tgaccccgag ttggtcggcc
    13381 agatccgatg cgtgcagccc gccggccgcc agcggtacca gcgcaaccgc ttgagccgga
    13441 cggattccgt cgagcccggc cgcggcgaag gccgcccgca ggtggggcgc cgccgcggcg
    13501 gccaccaagt tgaccagcgc cggcacggtg ggttcccact cgccattcag aacacgtcgc
    13561 atggccatga gtttgccatc catcccatga ttgacaagcc gcttgtcaaa tgtcttcaat
    13621 cctgccagga tggggttcat gcgatgcgca cgattgctcg gcgtctgtct ccttccggtt
    13681 ctggtggtcg ttctcggggg gtgcctgggc ggcggccacg cattgggaat gccaggcgcg
    13741 caatcgatcc cggtcggaca gtccacccag accgtcgagt cgggcggtgt cgaccggacg
    13801 tttcacctgt accgaccgca gggattgacc gatgccgtgc cactggtagt gatgctgcac
    13861 ggcggcttcg gcagcggcga acaagctgag cgttcctacc attgggacaa cgaggccgac
    13921 ggcggacatt tcctggtcgc ctaccccgac ggtctcaacc gcgcctggaa tgccgggacg
    13981 tgctgcggcg agccgcaacg cgtcaacgcc gatgacgtcg gcttcatcac cgccatggtg
    14041 ggcgccatcg agcaggagat gcccatcgat cgcgcccgcg tctatgtcac cggcatgtcc
    14101 aacggggcga tgatggcgct gcgactgggc tgccaaaccg atacattcgc cgcggtcgcc
    14161 cccgtagcgg gcacgctgct gaccgactgt tcacaggcac ggccggcatc ggtgctacag
    14221 atccacggaa cggccgacga cagagtcccc tacaacggtg gtcccggaaa agccttcgct
    14281 agcaacggga atcctcgcgt cgacggtccc ccggtggagt cggtcaatgc cacttggcgt
    14341 gcgatcgatg cgtgcgggcc gccgaccaca agcaccgcgg gagacgtgac gaccgaaacg
    14401 gccggatgcc cggatggacg gaccgtggag ttgatctcgg tggccggtgc gggtcatcag
    14461 tggcccggtg gcgagccgag cccgctggcg gaactcgccg gaattcccgc tccgtcgacg
    14521 gcgctggacg ccaccgatac gatctggcaa ttctttgcgc agagccatcg ctgagacggc
    14581 gctcgccccc ctcgcgaccg ggtggtgatc cgtaggctgg tttgtgctgg ccgatgtcgg
    14641 gatgatggcg gggttcgagt tggtgcgatt ttcgggatgc gcactgtggg catgtgcggc
    14701 gttcaccgtc atgctgtgcg gttcgtgcgc cggtccgcca cacgggggta caagttccca
    14761 ccagacaccc gggaaaccac tcgggcacag cggatgtggt ccgccgatca cgtatcaggt
    14821 cttccagcaa ggcgcttccg gaagcaattg gtcgaaggcc aacgaccgca tcgcattcaa
    14881 cagcaaggca agcgatggcc gattccacat ctataccgtc aagccggatg gcacgaaccg
    14941 gcagcagctc ggagtcggca gcgcgacctt cccgcaacgc accaccggta cgcccgcgtg
    15001 gagcccgtcc gggcgattcg tcgcgtttgt cgccgaaaag tccaatcacc caggcaattc
    15061 ggtgggcgcc acccccggat ggggaagcta ctcggacctg tgggtggcta ccgccgacgg
    15121 ttctcacgcg tggcaactca ccgacgtgcc gaccgacaaa gatcacggca cgatcagccc
    15181 cgagttttcg cccgacggcc gcctgctgga gtggaccgaa cgcaccgccg cggccaaggc
    15241 cctcgatccg aatcgcttcg ccggcttctg ggtgatcaag gtcgccgatt tcgttgtgtc
    15301 ccaggatggg acgccgtcgc tgtccggtgt ccgcacgatt tcacccgcag gcgacgcgtt
    15361 caacgagacc ggggggttct ctgcggattc cacgtcgctg gtgtttacca gcaacttcga
    15421 aacccacaac ttctggaaaa gccagatcta tcgcctcgac ctgaactccg acgcaatcgc
    15481 tcgacttacc cgcggcgaca gctacaacga acacccccgc tacacccccg acggccaggt
    15541 tctctggatg accaatgccg acaacccgtc gcacggcacc gactggtgga ccatggatgc
    15601 cgacggttca catcaacagc gcctcagcga cttcaacagc gacggcgacg ccagaggttt
    15661 tggaagcggg cgggtctggg ctacgaccgt gcagaccgac ggctggagcc ctgacgaacg
    15721 ctacttttac ggcgacgtgg agaccaacct ggtgaactcc gacggcgtga tcctccgcgc
    15781 ttcactcacc tgcgggggtt cgtgagcctc aacgttcgct cagcatcgca tcgatgagcc
    15841 ggtgcagcac ctccccggtc tcccgatggg cacgcttggg atcgtcggcg gtggcgatcg
    15901 ccatggccgc ctcgtcgagc gcgccgatca gcacatgcgc caacggtcgc accggttgat
    15961 gggccagctg gccggccgcc atggcctggg tgagtaactg ctcggtcatg cccaggctgt
    16021 accgctgggc cacatcacgg aaggcggacc agccgagcac gctgggcgca tccagcagga
    16081 tcagctggcg cacctccggg tcgccggaga cgtccagcca ggcatcgacc gccgcccgga
    16141 ttgcgtcagc ggggctcgtc gcgccggact tggtcaccag cgtggccatc cgggccatca
    16201 catcctgctc cacgacctcg acgacctcgc tgaatagcgc tgccttgtcg gcgaattggt
    16261 gatacatcgc gccgcgggta acccccgcgg ccgtcgcgat ctccggcgtc cccacctccg
    16321 cgtaaccccg cagcccccac aacttgcggg cggccgaaat cagcgcgtcg cgggtcgccg
    16381 cggagcgctc ctcttgagtc cgtctcttga tttccataca acctgttggt aacttacaaa
    16441 cagacagtcc gtttgtaaat attctagagc aggagctatc tatgccgacg atcgacatta
    16501 atgccggaac catccattac gaagcaaccg gacccgagaa cggcaggccc gtcgtgttcg
    16561 tgcatgggta catgatgggc ggccaactgt ggcgccgggt gagcgaacga ctcggcgacc
    16621 agggcctgcg gtgcatcgcc cccacctggc cgctgggcgc gcaccccgag ccgttgcggc
    16681 acggcgccga ccgaagcatc acgggcgtcg cgggcatcgt cgcggaggtg ctcgacgccc
    16741 tcgacctcga ggacgtcgtg ctggtcggca acgacaccgg tggggtcgtc acgcaactgg
    16801 tcgcggtgca ccatcccgac cggctgggtg cccttgtgct cacgagttgc gatgcgttcg
    16861 aacactttcc gccaccgatt ctcaagccgg tgatcgtggc ggccaagtcg aaggcgctgt
    16921 tccgtaccgc gatacaggcg atgcgcgcgc cggtcgcgcg caagcgcgcg ttcgacgggc
    16981 tggcgcaccg caacatcgat gacctcacgg aggtctgggt gcgtccggtg ctgtccgatc
    17041 cggcgatcgc cgaggacctg cgccagttca ccctctcgat gcgcaccgag gtcaccacgg
    17101 gcgtcgccgc ccggctgccc gagttcgaca agccggcgct catcgcatgg tcggccgacg
    17161 acgtgttctt tgaggccgca gacggcgaac gactggccgc gaccatcccg aacgcccgcc
    17221 tcgaggtgat cgagggagca cggacgttct cgatgctgga ccagccggag cggctcgccg
    17281 atctgctgtc gacgatcgcg gtgcgcgcct agcgattcca ccggtcacgg tcggcggacg
    17341 ggttgcggcc ccgggccacc cgctaccatg agacggaccg tctcgtaact gggggtggac
    17401 ccgggttgat ggctggcgac acgattcagg cgctcctgca cgagcgcttg tccgactcgt
    17461 cggtagccgt gaaacacggt aattcgcaat ggacttggcg tcagtatctg acggagtcga
    17521 cgacgcgggc cgcggcactg atcgccgccg ccgatccgca acgaccgatg cacatcggcg
    17581 ccctgctggg caacacgacc gagatgctca tacagatggc agcggccggg ctcggcggct
    17641 acgtgctgtg cggtctgaac accacccgcc gcggcgaggc actggccgcc gatgtccggc
    17701 gcgccgactg ccagttcgtc gtgaccgacg ccggacaccg gccgctgctg gagggtttgc
    17761 acctcgggga gacgcagatc ctggacacct cgacaccgcg gtgggcggaa ttcgtcggcg
    17821 gtgccggtga actggtccca caccggcacg tcaccgcgat ggacccgttc atgatgatct
    17881 tcacctctgg aacgagcggg aatcccaagg cggtccaggt atcccacctc atggccacga
    17941 tcgccggtct gagcctgacc gagcgtttct ccctgaccga gcgggacacc tgctacgtgt
    18001 ccatgccgct gtttcactcc aatgcggtcg tcgccggatg ggcgcccgcg gtggcctcgg
    18061 gcgccgcggt ggtgccggcc gagttctcgg cgcgcaactt cctcgacgac atccgccgct
    18121 acggcgcgac gtacatgaat tacgtcggca agccgctcgc ctacatcctc gccaccaccg
    18181 aacgcgacga tgacgcggac aatcccctgc gggtcgcgtt cggtaacgag gccaacgaca
    18241 aggacatcga ggagttcgcg cgccgtttcg acgttcacgt cgaagacggg ttcggctcga
    18301 ccgagaacgc cgtcatcgtg atacgcgaac cgggcacgcc gcggggctcg atcggcagag
    18361 gggtcgacgg gatcgcgatc tacgacagcg agaccgtcac ggagtgcgcg gtcgcgcgct
    18421 tcgatgcgaa cggcgcgctg gccaacaccg acgaagccgt cggcgaattg gtcaacaccg
    18481 cgggatcagg ctttttcacc ggctattaca acgatcccga agccaacgcc gaacgcatgc
    18541 gccatggcat gtactggtcc ggcgacctcg cctaccggga ctccgaaggc tggatctacc
    18601 tcgccggccg taccgccgac tggatgcggg tggacggcga gaatctggcc gcggcgccaa
    18661 tcgagcgaat cctgttgcgt cacaaagcga tcaaccgcgt cgcggtgtac gccgttccgg
    18721 acgagcgggt cggcgatcag gtgatggccg cgatcgtcct cagtgcgggc cgcacgctcg
    18781 accccgatgc gttcgaagca ttcctcgacg cgcagcccga tctgtcgccc aaggcccggc
    18841 cgcgatatgt ccgcctttcc gccgacctgc ccagtaccgc cacccacaag gtgctcaagc
    18901 gccacctgat cgcgcagggc acggccatcg gcgcgggaga agtgttgtgg gagcgcgaac
    18961 cacgcgggac ctcctaccgg cacgcggcgc ccggtccttc gatcggctcg gtttcccagg
    19021 aagccgactg aagaccgggt cggttcgcag attgcaggat cgcgcattgc cgacggttct
    19081 cgcgtgccac catggggcat gccaaggccc gcacggtggt gcagttgagc acgaacaaga
    19141 ctgtgcggcc caccaccgcg gacgtggccc gattggccag cgtgtcgacc gcgacggtca
    19201 gctacgtact caacaacgcc gaaggccgca gaatctcggc gcaaacccgg gaggcagtgc
    19261 aacgcgccgc ggagctactc ggttatcgac ccaatcttgc cgcgcgcaat ctcgcccgcg
    19321 gcaaaagcgg tgtggtcctc tacgtcgtgc cgcacgtcgc ggtgggcgag atgccgatgc
    19381 aggccggcag ccggatgacc accgaactgg cgcgctcggg tttgcttcag gtacagatct
    19441 tcgaaaccga ggacgatcgg catatcgtcg acgcgatcga ggtcctggac ccgatcgcgg
    19501 tcacaagcct tttcccgctc agcgggcccg ccttcgacgc ggtgcgaacc gcgggcatac
    19561 cccacattga gattgggacg ctgcccgcgc tcggagatcc acacctttcg gtcggcgaaa
    19621 tgcgggtcgg tcacctcgtc gcgcgtggcc acacactgat cgcgttcgcc tacaccggga
    19681 tcgcgaagtg gcgtgcgctg ggcgattact ggttcgaggg tgtctcgcgc gccgcgcggt
    19741 tgcgtgacct tccgcccgtc cgcgtcggtg acatcacccc ggccaacgcc gcggacgtgg
    19801 tgaccggttg ggtgcgcgac ggcgtgaccg cggtgtgcgc acaaagcgac gagatagcgt
    19861 gcctggtttt gtacggcgtt cacgaagcgc ggctgcgctg tcctgacgac ctcgccgtga
    19921 tcggcgtgga tgccagcccc gtcggcgcgg tcagcacacc acctctgacc agcgtggaat
    19981 tcgacccgcg cgcggtcgcc gacgtcgcgg tcgatgccat cttcgagcgg ctggggctcg
    20041 cgcgaccgcc ggctggcgca ccgaccgaaa tcgcccgcct catcgtgcgc tcctcgacct
    20101 agcgcggccc tagccctgcc gtcgcgggac cgcacgtcga ccgccctgga catcctcagg
    20161 ttaagcgcat aacctacgcg ttagcgaggc ggatactcaa ggaggagtac ccatgtcggt
    20221 acaggctgtg ctggacgaga tccgtgaccg acccggaacc ggggagacga tcgcggtttt
    20281 cgaccccgcc accgaagaac aggtcaccga gttcaaagac tgcggtacgg aggccgtcga
    20341 cgacgccgtg gcccgcgcga aggcgtcctt cgagtcgggt gtctggcggg ataagcctcc
    20401 cagcgagcgg gccaagatcc tgtggcgtgt cggcgagctg atcgaccaga atgccgagct
    20461 gctggccgac cttgaatcgc tcaacgccgg catgaccccg ctgcaagcgc agggaaccgt
    20521 gaccgtgggc tcggaattct tccggtacta cgcaggctgg tgcaccaaga tcgaaggcat
    20581 cgcggccgac gtgcacaccg gcggtctcac gggcatcgac tcgcatcagc acgtgtacac
    20641 cctcaaggag ccttacggcg tggtggggct gatatttccg tggaacgggc cggttttcaa
    20701 tttctgcgcc aagctggcgc cgtcgcttgc cgcaggctgc agcagcgtcg tcaagcccgc
    20761 cgaggagacc ccgctgtcag cgttggtcct ggacaacatc ctgcgtgagg ccggggttcc
    20821 cgaaggggtg gcgaacctgt tggtcggcta cggacacacc gcgggtgcgg cgatcaccgc
    20881 gcatcccgac gtcgagaagg tcgccttcac cggctcgacc gaggtcggca gggcgatcgt
    20941 gcacgcggcc ggcgaaagca acctgaagaa agtcacgctc gaactgggcg ggaagtcacc
    21001 ggtggtggtc ttcgatgacg ccgacctgtc gaaggccgtc ccgatggcgg cattcgggac
    21061 cttcatccac tccggtcagg cgtgtgtgtg cggatcgcgc atcttcgtgc aacgcggtgt
    21121 ctaccagcaa ttcgtggaga gcctggcgac cgtcgcagac gggctgccgc aaggcggccc
    21181 caaggacgag ggcagcctga tcggcccgtt gatcagtcag aagcagctca cccgtgtcct
    21241 gggttacctc gaccagggca aggccgatgg cgtcgaggtt gtcaccggcg ggtatcgcct
    21301 cgaccgcaag gggtacttcg tccaccccac cgtgctcacc aacgtcaact ccacgagcag
    21361 gctgtttcag gaggagatct tcgggcccgt cgtcgcggtc ctgccgttcg acgacgagga
    21421 cgaggcggtc gcactggcca acgacagcac ctacggactg gccgccaccg cctggaccaa
    21481 agacctgggt cgcgcacacc gaattgccaa gcggctcaaa gccggaaccg tggggctcaa
    21541 ctgtcagatg cagttcgatc actccatgcc gttcgggggc tacaagcaat ccggttgggg
    21601 ttacgaatcc ggcaaagcgg gtctggacac ctacctgcaa acgaaaattg tttgggcgca
    21661 gatgtaatcg gcgctagcgc ggaaaagccc gaggcaaagt caagtggttg gtgcaacggc
    21721 gatttcgccg caagttcacg cctgatcagc ccgctacgga ggcctcatag atcgactgaa
    21781 tggccttgtc cagcgtcgca ttgaactgct cgtcggactg gtcggcggac agtccctccg
    21841 tcagcgcgcg gctgaagctg gcgatcaccc cggtgttctt cgccagcagc tcgtcggcct
    21901 cctcacggga gtagccgccg gagagcgcca gcacccgcat caccttggga tgttcgacga
    21961 aggcgcggta gtggttgacc tcgctcggca aactgagctt gagcatcacc cgctgcccgt
    22021 cgggcacggt ctcgagttgt ttcgtgatct cgtcgcgcag gatgtcctcg gccgcagcct
    22081 tgtccgggat cgagatggtg acctcgggtt cgaggatcgg caccaaaccg tgcgacagca
    22141 cctgttgcgc cacctcgaac tgctgggcca ccaccgcggc gacgcccgcg gtgttcgcgc
    22201 caccgatcac cgaccgctcc ttggtgccga aaacaccctt ggccacggcg cgctgaagca
    22261 agtcgtccag tccgggcatc ggcttcatca actggacgtc gtcggacgcc tccgccaacc
    22321 ccttgtcgat cttgaggatg ggcaccacgc ccttggtctc ccagaggtag gtggtcgacg
    22381 gctgaccctc gatctcgcgg tccatggtct gctcgaacag gatcgccgcc agcacccggt
    22441 cgccggtgaa ggccggggag gtgatgatcc gcgcgcgcat ctggtggatc aggtcgaaca
    22501 tctcctcctc ggaggagtac gcgtcgtcct tgatgccgta cagacgcaat gcctttggcg
    22561 tcgaaccacc gctctggtcg agcgctgcga cgaatccctt gcccgacgtc atccggtcgg
    22621 cttgctgctg gttgggcata gaattcccca ctccctcgtg gcaccgtcgc cgacggcgca
    22681 ccgtcaagct cgaatcgtgc cgatcatatt cgtccggtgg cggcgcgggc cgccaggtag
    22741 accgtcagcc gcgggcgact tcgggctgcg cgatcacatt gctgataccg gtgacaccga
    22801 gccgctcccg gaaagcctgc gccgtgtagc cgaccgccgc cgcgtccgtt cgggcgcgca
    22861 ccgtcgccga ccgtggcatg tgaaacagca cgcccatttc gccgaaatag tccccgggcc
    22921 cggccacttt gaccaattcc tcgccgccgt cggccaattc gcgcacgatg tcgaactcgc
    22981 cgtcggacac cacatagatc aggtcgccca tcgtgcactg ctcgaacaac acctcgcccg
    23041 ccttgagctg cacggtttcc ggtagccgat tcgaggacgc gtgcgcgggc gccagctcca
    23101 ccacgtgatc ggccatcggc agtatccggc tgtcgtgggt cgcgaccacc accacccgct
    23161 cgccctccgc gagcgagcga atcaaccgca gcacctcctc cacctggatg aagtcgagat
    23221 gcgcggtcgg ctcgtctgcg aggatcaacg gcggttccag cgcgatcgcg cgagcgaccg
    23281 cgacgcgttg ctgctggccg ccgctgaggt caccgggccg gtgtttcatc cggtccgcaa
    23341 gattgacgcg cgtcagcagt tcctcggcgc gccggcgcga ggccgcgcgt gaccacccgg
    23401 ccgcgcgcaa cggcaccatc acgttctcca gcgcgctgag gctgggtacc aggttgaagg
    23461 cctggaacac gatgcccacc gtgtcgcgcc gatacttcgc cagctcccgg ccgtccagcg
    23521 cggtgacgtc gatgccaccg aatttgattg ctccactctt cggccgcagg atgccgccga
    23581 ggcacgacag caacgtcgtc ttcccgcagc cgctggggcc cagcaggatc accagtgacc
    23641 ctgcggccac gtccagattc agcccgttga tcgggcgcag cgcgtaaccc ccactggagt
    23701 actcgacgac gaggtcttga atgctcagat cagccatcga tcagggaccc ccgaacgcga
    23761 gcgcgggatc gaccgccacc gcacgccgga gcccggcaac gctggcgagc agaccgatcg
    23821 cgatggcgat ccccggcagc gcgaagtagg cacgcacggg caccacgacg atcatcggga
    23881 acaccggcga cagcagctgg gcaaggagaa cgcccaacac cgccgcgagc agcgcgacga
    23941 caaccgcctg cagcgcgagg ccggccagaa tcgaccgcgt cggcacaccg atcgccttga
    24001 acaccgcgaa gtcgcgcagg cgctcgagcg cggacagata gaccaacgag ccgacgatca
    24061 gcacagccac gatccacagc aacaaggcca cgatcgagat ggcacccacc gcgaccttca
    24121 gcggtcgcaa cagatcgttg acaccggccg ttcggtcgac ggcctggtag ccgtccggga
    24181 gctgtcgggg cgtgccgatg atgccgatcg acgtgatcat cggctgcccg ttgtaggaca
    24241 gttgctgtag accctcggtc gtcaggaaga ggcagggagc cttggccagt gcggtggcat
    24301 tgggcacgat gccgacgatc tgcagcttgc gcgcgccgac ctgcaactcc tcgccgacgt
    24361 gtttgcccag cgtgctagac accgcgacct cgccgggcaa cgtcggcacc tgcccctccg
    24421 agacttgtgg catgccgggt ccgtgcgcag gcgcgccgaa cgcggtggcg ttgcgcgacg
    24481 aggtgccctc cgtgatggtc gcgggcgcgg tggccagcgg ggccgcggcc accacccccg
    24541 gctcggcggc cacccgggcc atgtccacct gagcgaaggg tgtcgaaccc aggaaaggtc
    24601 cggcggcgcc ggccttgacc acgaaggcgt cgacacccat ggaatcgacg gtgcgctggg
    24661 cttcgacccg gaagccgttt gcgagcccgg tcaggaccag cgtcatcgca aagaccagcc
    24721 cggtgctgac gatcgcgatg acgaggcgcc gccttcgcca ttgcatgtca cgcaacgccg
    24781 ccaacagcat gctcacgacg ctaccgctgt gaccccttcg cggacagagg tgtctttccc
    24841 ctcggcaggg tccctgcccg agtagctcgc ggtcgcgtcc ttgaccaccg tgctgtccgc
    24901 cgtcacggct gggttggatt ttgttgcggc gcaatagttt tggtgggcca cagtgccatg
    24961 cggtcgagga gccggtcgcg cagcaccagc gtgtggaaca acaccgcgca gacgtgggcg
    25021 gtgaacgccg cgaaaagcaa gaaggcgcag acggtgtgcg cctgccgaag cgccgcatag
    25081 acgtcgatgt cgtgcggcgc gatgccgggc agcgcaagtg gccccatcag gatgaccgga
    25141 aaacgcgccg ccgacagcat cgcccacccg atcaggggct gaacgagcaa caacgcgtag
    25201 agcaggtatt ccgaccagga cgcgacgcga cgttccgcgg ggctcatggt tgccaagaac
    25261 ggcgggagcc ggtgggtgag ccggttgacc agccgcacca cggcgaaggc caagatcagt
    25321 atccccaacg gccggtgtat ggccagtagc agcgggtaat acgtcagaga ggcgatcatc
    25381 gtcaccccga tcaacagctg agcgatgacc atcggcgcca tcagccagtg caggatccgc
    25441 gacggaaggg cgaatcgggt tggtgcggtg gcggtttcgt ttgccttgcc ggtcatgaga
    25501 gcacccgggc cacgttgacc tcgctggccg acttgggttc ctcggcgcgt cgggtgaagg
    25561 agcgtgcgta cacggccgat cgcgccgcgg gcaggggatc gtcggacgcg gtgatgccgt
    25621 cgggcagcac caacgggtcg aaattgatgt cgcgcccgtt gccggcttcc tcggtctgca
    25681 ccgcgttgat ggtgaccgtg ccggcatcga tggaccgccg cgactgcggc cacggtgtgg
    25741 tggcgtcgtt ggtcggatcg cccggctcgc cgaccgtgag gaccagccgc caccggagcg
    25801 gccgctgcgc caccgcgcgg atgaggtcgt cgaacagaaa gtccttgccg cgtgacgggg
    25861 cggggggccc gccggcatcc tgcggcacca ccgaccaacg caccggaacg ctggcgccgg
    25921 cgctgttggt gaaccaaaaa gcattgagcc cataaaaagt gctgtcggca aatccggcac
    25981 tgggcggtga ctgcttgatg atcttcatcg ccgcgacggt ctcggggtgg cgatcgagaa
    26041 atgccgccat cttctgtggg tcgggttttc cggtgtcggg cagcggcttg gatgcgagca
    26101 accgctcgta aaacccttgg ggcgtgctgt cggtgaaaac gggaaagttg atcatcgcgg
    26161 tgcgccattg cgcaccgtcg ggcggctgga acaccagtcc cagcccgcga accgtctcgg
    26221 gtttgtcggc ctgatccggc aggccgccgc ccagcgaaaa ccggccgacc agcggaacgc
    26281 tgcccggccg gaagacggct gcccggcaga tggcagcgcc cgccccgctg ctcgcgaatg
    26341 accccgtggc gctgaggcct ttggcatggt ttcgccgaaa tccgtcgtgg tggccataga
    26401 cgtcttcgaa gcgatcggcg aaccgtgacg gggtgagcga agccggcgcg tccggccgca
    26461 gccagccacc ggcataggca aacccgccta catccaccgc ggcgaaaccg gccactgcgc
    26521 ccatcccaag caatgcggtg cgccgcgtca acgcggtgcg ccaggaggaa tcggtgggct
    26581 tcgtttctat gggcttatcg gattcgtcgt ccggctcgtg gctacgacca ccgtgatcgc
    26641 caggctccat acccaccacc cttgcgcttt acgtgccgag ccgcgcggtc tgaaaaaaaa
    26701 tgtaggcaaa gcaatttgcg gatgggtgag cgcggcgcgc gccgttgcgg cggcccacac
    26761 acccaacccc aaccacggca cggcgccgaa attcgttcag ttcgcgcggc acaatgccgg
    26821 atcagttgcc ggcgagcttg cggtgctcaa tcagatcgcg cacgcccccc gcaccgaggc
    26881 gctcgcggaa cgccggcgcc gtaaagccga ccgccgtcgc gtcggtgcgg gcgcgcgccg
    26941 tcgccgagcg cggcatatga aacagcacgc cgatttcgcc gaagtagtct cccggccccg
    27001 cgactttgat caattcctca ccgccgtcag ccaattcgtg tacgagttcg aactcacctt
    27061 tggacaccac gtagatcaac tcgcccattg tggactgctc gaacagcacc tcgccggcct
    27121 tgagctccac ggtttccggt ggcttattcg tctcggggaa atcgggcgtc agttcgacga
    27181 cgcggtcagc cattggcagc atccgggcgt cgtgggtggc gaccaccacg acgcgatccc
    27241 cgtccgcgag ttcgcggatc agccgcagca cctcctccac ctgaatgaag tcaaggtgcg
    27301 cggtgggttc gtcggcgagc accagcggtg gatccagcgc gaccgcgcgg gcgatggcga
    27361 cgcgttgctg ctgcccaccg ctcaggtcgc ccgggcggtg tttcatccgc tcttcaaggt
    27421 tgacccgggt cagcaattcc ttggcacgcg tgcgcgcggc ccggcgcgac atcccggcct
    27481 cgcgcatcgg aaccatcacg ttctcgagag cggtgaggct gggcaccagg ttgaacgcct
    27541 gaaagacgat gcccaccttg tcgcggcgat agttcgccag cgcggcgccc tcaagcgtcg
    27601 tgatgtcaac gtcatcgaac ttgattgccc cagactttgg ccgcagtatg ccgccgaggc
    27661 aggacagcag cgtcgtcttc ccgcaaccgc tgggcccgag caggatcacc agcgaccctg
    27721 cggtgacgtc gaggttcaaa ccgttgatcg gccgcagagc gtatccaccg ctgtcgtact
    27781 cgacgacgag gtcctgaata ctcagatcgc ccaccgctag ggacctccga acgcgagcgc
    27841 tggatcgacg gccaccacgc gcttaagtcc ggcgagactg gccaacaggc cgatcacaat
    27901 cgcgaccacc ggtaacagca ggtaggcacc ggcgggtacc gcgacgatca tcggaaacaa
    27961 cggtgccaac agctgcgcaa ggatgacgcc cacgatcgcc gcgagcagcg cgacgaccag
    28021 cgcctgcatg gccagacccg ccataatcga gcgcgtccgc gtgccaatcg ccttgaacac
    28081 cgcaaagtcg cggacccgct cgagcgcgga caggtagacc accgatccga cgatcagcgc
    28141 cgccaccacc cacagcaaga cggccacgat cgagatcgac gtcaccgcga ccttcaacgg
    28201 gcgcacgaga tccttgacag cgcccgttcg gtcgtagctc tggtagccgt ccgggagatg
    28261 tcggggtttg ccgacgaccc cgatcgacgt gaccatcggc tggccgttgt atgccacttg
    28321 ctgaaggccc tgggtcgtca gaaagatgtt ggggatcttg gccagcgcag tcgaattggg
    28381 cacgatgccg acgatccgca atacgtgagc accgacctgc agggtgtcgc cgatgtgtcg
    28441 gtccagcgtg ctcgacgccg cgacctcgtc cggcttcgac ggtgcctggc cctgcgacac
    28501 gtgcggcatg ccgggtccgt gctcgggcgc gccgaaggcc gtgacgtttc gcgttgacgt
    28561 gccttgcttc atgatcgtcc cgacacaggc cagtggggcc gcagcgacaa cgccgggctc
    28621 ggcggcaatc cgcgccaggt caacctcggg gaacggtgtc gaacccagga agggtccggc
    28681 cgcgccgtct ttgaccacga atgcgtcgac gcccatcgag tcgacggtgt tgcgtgcctc
    28741 gactccaaag ccgttcgcga gcccggtcat cacgagcgtc atcccgaaga tcagcgcggt
    28801 gctgacgatc gcgatgatga ggcgccgctt tcgccattgc atgtcgcgca gggccgcgaa
    28861 gagcatttcc agaacctacc aacgtcccta tcgtccggga cagagttttg cctacgcact
    28921 gtctgcgctg atcaccgcaa agagtcatgc ctggatcgag gctgcgcgtg tcggtgtcaa
    28981 cgtggcttac gatcgaccaa acacgtgcgt gctgcgccgg ccctcggcgg cggagaatcg
    29041 acggagtgtt cctgctccat caagcgtgtt ctgcgggttg agtcagcagc ccgggccggc
    29101 aatggcgatt gccggccagc agtgtccccg caggggcgaa tgtgagtgag aaatcatggt
    29161 cgaacaatcc ggcgattccg tcgatccgac gagcttcgag gtagggaaac accaggtaga
    29221 tcaggccgtt gtggtcacgg tctcgggtga agtggacatg ctcagcgcgc cccggttggc
    29281 ggaggcgata agcactgcgc tggccgctgc accggcggcg ctgatcgtcg atctgtcgaa
    29341 ggtggatttt cttgcctcag ccgggatgac ggtcctggtg accgcacagg cccaggtggt
    29401 gccgccgacc cgattcgccg tcgtcgccga cggcccggcc accagcaggc cgctgaaact
    29461 catggggtta gacaccgttt tgtcgcttta tagcgcgctc gatagcgcgc tgagcggcgt
    29521 cgacggtggg cgaacctcgg cagagtgagg acgcgtgtct tacatcgatg accgatcccg
    29581 cttcatccgg acgcgagtcg ctgccgatgc gcgcagcgcc gcccggaccc gagccgagtt
    29641 cgggcgctgg ctcgacaggc atttcacgct tggtgcccaa aggttcagcg acctgctgct
    29701 ggcggtctac gaagcgattg cgaacgccgc ggaattcgcc tacgtcgact cctcgctgcg
    29761 caggacgatg gatgtcagcg cggcctacga cgtcgaatcc gacaccctcg cggtgaccgt
    29821 cgatgaccgt ggccgctggc gtcagagccg tccggaaccg gcgaccgtgc cgcgccggac
    29881 acagctgcgg gggcggggta tccagctgat gcgagcgctg gccgatcagg tgaccatcga
    29941 ccgcacgcca cagggaacgc atgtcacgtt gacctggatc gagctgacgc ggtaggggcc
    30001 agaccggcgc gggaggaccg gccccgcgtc gaggggcatt cattgacttc gatccatcag
    30061 ccggaatatc gtcgggagtt acgctacgtg gcgtagcgaa tcgccgtgcg tctcgcctcg
    30121 acagcgcgcg gcgacaagga gagaccctca tgttcgacct gaagatcacc ggtggcaccg
    30181 tcgtggacgg caccggcggg cagcgctacc gggctgacat cggcatcaag gatggcgtga
    30241 tcgtcgacgt cgtccgccgc gacgccggcg acgcggagcc gagtggcctg gagactgccc
    30301 tggagatggc cgacgccgcc gaaacgatcg acgcgacagg gcatgtggtg gcccccggct
    30361 tcgtcgatat ccatacccac tacgacggtc aggtgagctg ggacagcctg ctggagccgt
    30421 ccagcggtca cggcgtcacg acaatcgtga ccggcaactg cggtgtcggg ttcgccccgg
    30481 tgcggcccgg cagtgagcaa tggctgatcg agttgatgga gggtgtcgag gacatccccg
    30541 gcaccgcgct gaccgagggc atcacctggg gctggcaaac ctaccccgag tacctcgacg
    30601 cgatcggaaa gctgaagttc gcgatcgacg tgggcagcca ggtcgcgcac ggcgccgtcc
    30661 gcgcctacgc gatgggcgag cggggtgccc gcaacgagcc ggccaccccg gaggacatcg
    30721 cggcgatggg ccggttggtc accgaagcga tcgaagccgg ggcgctcgga ttttcgacat
    30781 cgcgcacgct gggccatcgc gcgatggacg gcgaacccgt acccggtacc ttcgccgccg
    30841 aagaggagct gttcggtctc ggccgcgcca tggcggccgg gggccaggcg gtgttcgagc
    30901 tcgccccgca ggggtccgcg ggtgaggaca tcgtcgcgcc gaagaaggaa ctggactgga
    30961 tgcggcggct gggcggcgag atcgaccggc cggtgtcatt cgctctgatc caggtggacg
    31021 ccgatccgaa gctgtggcgc gagatgctgg acctctccgc agacgcgcac gcggcgggca
    31081 gtcggttgca ccctcaggtc gcggcgcgcc cgttcggcat gatgatcgga ttccagggcc
    31141 atcacggctt cagccaccgc cccacctacc gcggcctggc ggccgaatgc agccgcgagg
    31201 aactcgcgca gcgcctggcc gatccggcgg tcaaggccgc gatcctgtcc gaggacgacc
    31261 tgcccgtcga cccgaccctg ttgttcgacg ggatgttcgc cctcgtgcag cactccctga
    31321 aacggcttta cgcgctcggt gacccgcccg actacgagcc cactcccgac cgcaccgtcg
    31381 ccgccatcgc caaagcgcgc ggcgaggacc cgctgtcgac gctctatgac ctgatgctcg
    31441 agcggaacgc gaccgccatg ctgatgctgc cgctgttcaa ctacgccgac ggtaaccacg
    31501 acgcgatccg ggagatgatg ttgcaccccg ccggtgtgct gggcctgtcc gacggcggcg
    31561 cgcactgcgg gatgatctgc gacgcttcct atcccacctt cctgctgacg cactgggccc
    31621 gcgaccgaca ccggggcgag aagctgtcgc tggagtacgt catccgcaag caatcccgcg
    31681 acaccgctca cctcttcggt ctcaccgatc gcggcaccat cgaggccggc aagaaggccg
    31741 acatcaacgt gatcgacatg gacgcgctgc ggctgcaccc cgcggcgatg gccttcgacc
    31801 tgccggccgg cggccaccgg atcttgcagc gcgcaagcgg atacgcggcg actatc
//
LOCUS       DASSGQ010000240        26457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_897638,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000240 DASSGQ010000000
VERSION     DASSGQ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 26457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26457
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897638"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFI55_10490"
     CDS             <1..210
                     /locus_tag="VFI55_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET7074494.1"
                     /translation="ASILVVTHNGFCADGRDRPWWQLPIHRQLLIRTVVVPAGRIPPP
                     TRVGPWLERTWTQVDAWVAAHVEQS"
     gene            274..1026
                     /locus_tag="VFI55_10495"
     CDS             274..1026
                     /locus_tag="VFI55_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074495.1"
                     /translation="MEANGYFEKWLENVERRGVPSGVVIEIARRHAISPRSFEILETM
                     EEINDPRDKSYFLIPPGAGGQRARHATLMTYIVNAGTDYGRAGRRADFPETPYCAAEV
                     ARIINRQKANGWSYTRDVGFVDRNGGRLVTTPNGMLMGLGGNWIQRQFSQRGGTTWGD
                     IFMVNMGKVADPAQQLREIVGSGHAWHTLDLDRVLHHEERHSRQWAARGYPGMIRDYG
                     RELFRELAFGKTNRLEEDAGLSDGGYRASIPL"
     gene            complement(1002..1673)
                     /locus_tag="VFI55_10500"
     CDS             complement(1002..1673)
                     /locus_tag="VFI55_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HET7074496.1"
                     /translation="MAGSWGSVLGALIPLGLVIALSPLTIIPAVLVLQAPRPRPSGLA
                     FLAGWVLGLAALTALSVVGSGLLGGLHKSPPTWASWLRVVLGSALILFGFYRWFTRHR
                     NTESPAWMRTFATITPARAGLTGAVLVAIRPDVVLICVPAGLAIGGSGLDVADDSVAA
                     AFFVAIAASTVAIPILAYAAAGHRLDDTMARLKDWMEKNNSALMAAILVVIGAMVLYN
                     GIDAL"
     gene            complement(1749..2399)
                     /locus_tag="VFI55_10505"
     CDS             complement(1749..2399)
                     /locus_tag="VFI55_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HET7074497.1"
                     /translation="MLTKLIPLGLVIALSPITVIPAVLVLHTPRPRPASLAFLAGWVL
                     GLVGLTGVFVGLSDLLGDLGKAPPTWASWMRVVLGLVLIAFGVFSWLTRHRQTKTPGW
                     MRSFSKLTPVRAGLTGVALAPLRPEVLVLSAAAGLAIGTGGLSVAGAWIAGAVFVAVS
                     ASTVAIPILAFVAAGDRLDDALERLKVWMEQNHAGLTAAILVLIGLLVLYNGIHAL"
     gene            complement(2481..2789)
                     /locus_tag="VFI55_10510"
     CDS             complement(2481..2789)
                     /locus_tag="VFI55_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074498.1"
                     /translation="MGDLASIGYNGQPVRSPFRLVRPPVAVLVDLTVLFPREPHRSGR
                     YQPNGLQLHKVVEGRLSCWVICEQGDWWGLVTYPIEYGSKRRTVTHWVPAWTLRRKGH
                     "
     gene            3020..4462
                     /locus_tag="VFI55_10515"
     CDS             3020..4462
                     /locus_tag="VFI55_10515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7074499.1"
                     /translation="MGGAARPSFWYDEAATISASYSRSLGQLWHMLGNVDAVHGLYYA
                     FMHGWFQVFPPSEFWSRVPSGLAVGAAAAGVVVLARQFSSRTVAVASGAFCAILPRTT
                     WAGIEARPSALSMMAAVWLTVVLVGATRRNTAWSWLSYGIALAASVLLDAYLALVVLA
                     HLSFICAYQGTRKVLTGFVVTSILTVCTLTPFLLTVAGQAHQISWVAPIGHRTIEDVA
                     MQQYFERSPPFAILSALVIAAAIVLWLGASARLADGDRQLLSIAVAWLVIPTALIVIW
                     SALAHPIYTPRYLSFTAPAMALVLGVCAAAVGVKPWGTAALVGLFAIAAAPNYVLAQR
                     NQYAKYGMDYSQVADLIAVKAAPGDCLLVNDTVTFMPAPMRPLMAARPDAYRKLVDLT
                     LWQRATDRNDVFDTNLIPEVVAQPLSHCRVVWIITQADASVPAHEQGSALPPGPRYGP
                     TPAFAVPHDLGFRLVERWQFNLVQVIKAVR"
     gene            complement(4466..5302)
                     /locus_tag="VFI55_10520"
     CDS             complement(4466..5302)
                     /locus_tag="VFI55_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HET7074500.1"
                     /translation="MDRRSMLLTTGIGMLAAAAAVGATQAHASPTAPAAPGAPAAGAG
                     GPYIFADEFDGPAGAPPDPAKWTIQTWQDDVFPPVEGIYRDDRRNVFLDGNSNLVLCA
                     TQEMGNYYSGKLRGNFRSMINQTWEARIKLDCLFPGLWPSFWAVNEDPLPDGEVDIFE
                     WYGNGDWAPGTTVHAASNGKTWEGKSIPGLVDGGWHTWQMHWAEDGFHFARDGAPYFS
                     VPNKPIHVHGGAPDDMRWPFNAPGYWMTPMFTLAVGGVGAGDPAQGTFPCAMLVDYLR
                     IW"
     gene            complement(5513..5956)
                     /locus_tag="VFI55_10525"
     CDS             complement(5513..5956)
                     /locus_tag="VFI55_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7074501.1"
                     /translation="MSHSDEEPLGFLLYRVMAALRPQVAAELKPLELSLPEFVCLRIL
                     SNHPGLTSAELARGTNVSAQAMNQVLHGLEERGALTRPASSPAGRAMPARLTRRGKAL
                     LKRADVAVQVADQRILTRLTADEQRRLKQLLYAAGTRIGEDCANL"
     gene            6076..6717
                     /locus_tag="VFI55_10530"
     CDS             6076..6717
                     /locus_tag="VFI55_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074502.1"
                     /translation="MSGGFFGGFGGPGFGGHACGGSDGPGFGGFGGGWGGKGWGGPGF
                     EGGFGGGPGSGPGFGPGRRHFLKRAAFVTAALLLDGPADAAQIVQRVSDATQGAFTPP
                     QDVAELAIGLLAGRGVVTVDNGVATLTELGKNLLAWRGISSETAHAFLGRAAKFGDVF
                     KIRRTLFEIAGLARTIVWTGTDEQKEQLAETRTKVLEALTEAKKTLHRVLGED"
     gene            complement(6722..7747)
                     /locus_tag="VFI55_10535"
     CDS             complement(6722..7747)
                     /locus_tag="VFI55_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HET7074503.1"
                     /translation="MPDLPNRQIVLRRRPTGLLEPGDTELVTTPAPQVAAGEALVRTT
                     YVGLDAAVRTWLNDQPGYLPPVQLGEVIRAAGIGEVVASRCDAYEIGDVVTALTGFQE
                     YVIIRDDVFSTPIPGRAAVDQLAVMSVYGPTGATAYFGMMDIGRPQPGETVVVSAAAG
                     ATGSVAGQIAKIAGARVVGIAGGPEKCRAVVQDFGFDACIDYKNDDLAGALKEHCPRR
                     VNVYFDNVGGPILNAVLGRLATKARVVLCGVISSYLSGEHPGPANYVNLLAKTALMQG
                     FNALDEWGRFGEAFAALRQWENEGRIRHRQTVFEGVESCVDALNGLFTGANIGKMLVR
                     LSETARG"
     gene            7831..8538
                     /locus_tag="VFI55_10540"
     CDS             7831..8538
                     /locus_tag="VFI55_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3060 domain-containing protein"
                     /protein_id="HET7074504.1"
                     /translation="MNPQDDPEKRIRELERPLADAARASEPGEAHPPGGYAYPPAPAG
                     PPPPPPPPAPWTYGGAYSAPPSKPPSSNRMWWILGTVIVIGALVLAGAIAAFVANQLS
                     SVRSLITSPPTVSRTFVPPAITPTSPGPSASGTNTPSPSTSPTPPPGGELSVSGINAN
                     RTIACNDSIVDVSGVSNTVVITGHCASVTVSGVQNSVTVDAVDSIDASGFNNKVIYHS
                     GTPKVSNAGDSNVVQQG"
     gene            complement(8551..11718)
                     /locus_tag="VFI55_10545"
     CDS             complement(8551..11718)
                     /locus_tag="VFI55_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7074505.1"
                     /translation="MTATPLACHVCGTEPREGARYCDACGSLLTLVVDPAEYKQVTVL
                     FADMVRSMDIAAALGPERLRELVTDLVSQSMAVVQRFGGTVDKFTGDGIMALFGAPIT
                     LENHAFRACLAALELQQVAAQVAVEVRRRDGIDLQIRVGLNSGRVIAGEVGATHLGYT
                     AVGEQVGMAQRMESVAPPGGVMLSESTARLVEDRVVLGERETVHIKGFATAVPARRLL
                     GVDLEDRHSARQQSRLVGRKSEKQAVAELLDRACRGEGTIVTVTGRPGVGKTRLVRES
                     VVTATSRGYEVFVTYCESHTRDVPLHVIARLLRAIFGIAGFTAEEARARVRSQIPDAG
                     AEDLLLLDDLLGICGGPCPDVNPDARRRRLVDLINSVSQVRPAPAIYIVEDAQWIDGV
                     SEKLLADFAAAVPRMRATLLVIYRPQYSGALSRLPGAHPITLAPLSDSQIAELIKGLL
                     GEDPSVRGLSTVIADRAAGVPFAAEEIVRDLTERGELEGEPGAYVCIREVRDIHVPAS
                     LQAIIGARIDRLTGTAKRTLSAAAVIGAQFDLELLESLLDSVDVGPLIEAQLVEQVGS
                     TPRTTYAFCHPLIQQVAYESQLKAGRSQLHRRVAAVMQRTQAGFTGREAAIVATQYAA
                     AGDLRDAYDWHMRAATWYGARDIPAARKSWQQALRVADRLPADDPDRLDKRIAPRALL
                     CGSAFQVGGTPADTGFDELRELTTAAGDKRSLAIGMAGHLTTLTFNAHHCQAAAMASE
                     FATLVESIGDPEMTAGLFYAAAQAKWEAGEASESMRLAQRIIDVADGDPTMGSLVVGS
                     PLAWAIAVKGVAGMSLGRRGWRNDLYEGLVLARSVGGKTTRSFVELYRYAAAIQNGAV
                     LPDPHDVAQAAETLEIAQRSANNTAVAYALLNRATTLIRADSEDTAAGLEFLARAREM
                     IVDEQLIVTLRRMADIEIARERARSGDLDGAIDLATKVVVEQFDTGEMIFRGPATTAL
                     VEALLSRGSGADVMHARRAIDRLAAVPTEPGFVLHELPVLRLRALLARAHGDEPGYRQ
                     FVTRLRAKAAEADFEGYLAQAEAMA"
     gene            complement(11785..12171)
                     /locus_tag="VFI55_10550"
     CDS             complement(11785..12171)
                     /locus_tag="VFI55_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074506.1"
                     /translation="MGEYGAFGFDPEEFDRMIREGSEGLRDVFERIGKLVVGPGGRTS
                     WSAFFDDLSRRPRPEQETAGEAGDGVWAIYSVNADGGAHVEEVYATELDALRANKTNV
                     DPMRKVRFLPYGIAVSVLDGDSEEPA"
     gene            complement(12271..14217)
                     /locus_tag="VFI55_10555"
     CDS             complement(12271..14217)
                     /locus_tag="VFI55_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET7074507.1"
                     /translation="MYDPLGLSIGTTNLVAACNGTPPVSRRAVLTLYPHCAPKIGLSC
                     EDPSLIEAGMLMRGFVERIGDSVALVSPDGSAHDPDLLMVEALDAMVVAAGADAAASE
                     ISIAVPAYWKPGTVQALRNALRTHLGFVRSGMAPRLVSDAIASLTAANADLCFAATGV
                     IGLLDFGGTGTSATLVSLTGDFELVSPTLRYTAFSGDEIDQALVLKAFEELGHGSGID
                     PDSTAAVGQLGMLREQCRTAKERLSTETVVEFAAELGGRRCSMKLTRDELGDLIQDRL
                     SGFIYAFDEMLARHKKSWADLSAVVTVGGGASIPLVTERLSVHSRRPVVAPSQPAFAA
                     AAGALLLASRGEQLDIRTRTSIGLLTAGAASDVVQLPASDVLVIDGEALTDRELAWSQ
                     TEYPGDVRVPYTPAAFDEDGAAGWSMRLNLIEPPKEKPWRRLRLSQLIIGMCAVVAMT
                     AIGGVAYTLAGIENREAPPAPTVVPVPAPPASPLAPSPVPPPPPPPPSPVPSPSAEPV
                     PTPEPPPPPPPPPPPPPPPPPRVIVTTPPPVVTTRRPPPPPPTTTQPPPPPPTTVMTT
                     TPPPPPPPTSTPPPTPTTTVPMTTEWIHVPLLPVPIPVPVPQNQAPQPPQAPQYPQYP
                     GNSQYPGNSQYPGSSQNPFLGPGY"
     gene            complement(14372..15598)
                     /locus_tag="VFI55_10560"
                     /pseudo
     CDS             complement(14372..15598)
                     /locus_tag="VFI55_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979537.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15637..16293
                     /locus_tag="VFI55_10565"
     CDS             15637..16293
                     /locus_tag="VFI55_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7074508.1"
                     /translation="MSIFAGQKQSVGPTRRTSAPRKRGDDTRTKIIDETVRCIIEEGF
                     AAATAKHVAERAGVTWGVIQYHFGDRTGMLMAVVDDGVARLVDSLSSADVSELPLRER
                     IEVVVDTAWSCYSSPTSLAAFEILRATRGGPGASSRRHLLEMNSAIGQLGRLITEDPA
                     NAGVAEVIWAALRGVVLAQMITGTEIDWHCERRALIDMVSLYLQRPGGLPAAPPDVLQ
                     "
     gene            16280..16789
                     /locus_tag="VFI55_10570"
     CDS             16280..16789
                     /locus_tag="VFI55_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7074509.1"
                     /translation="MCCNDVMSPRHYGPGAAELADIEAIKQVKYRYLRALDTKHWDDF
                     ADTLAHDVKADYGPSIGEELHFTSRTDLVDYMRIALGPNVITEHRVTHPEIIVADDTA
                     SGSWYLQDRVIVPEFEFMLIGAAFYRDTYRRTEAGWKISSTGYDRTYDATMSLAGMNF
                     KIKPGRALA"
     gene            complement(16870..17523)
                     /locus_tag="VFI55_10575"
     CDS             complement(16870..17523)
                     /locus_tag="VFI55_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET7074510.1"
                     /translation="MRRLLASLCAAVCLSCLYVALAPVSGAASPWFANSVGNATQVVS
                     VVSTGGSNAKMDIYQRTTAGWQALAAGIPTHVGSAGMAPQARSEVPATPMGVYTLDSA
                     FGTAPNPGGGLPYTQVGPNHWWSGDDHSPTFNSMQVCQKSQCPFNTADSENLQIPQYK
                     HAVVMGVNKNKVPGGGAAFFFHTTDGGPTEGCVAIDDATLVQVIRWLRPGAVIAITK"
     gene            complement(17598..18221)
                     /locus_tag="VFI55_10580"
     CDS             complement(17598..18221)
                     /locus_tag="VFI55_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020784976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7074511.1"
                     /translation="MAALTAALVYAAMWLGYRQDWCWLHRVDWSLLNAAHDIAVKHPG
                     WVRFWDGVSFALGPVPVRVVGLATAVVALAKRNVRAALLLLTCGPLSGLVTTAAKGLV
                     NRPRPPTMLVPAPSTSFPSGHALEATSAVLALLTFLLPMMTRWMRRVAIAVAAFCVLL
                     VGVARVALNVHYPSDVMAGWSLGYLYFLLCLLIFRPRGWRSPGPPDP"
     gene            18326..18808
                     /locus_tag="VFI55_10585"
     CDS             18326..18808
                     /locus_tag="VFI55_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074512.1"
                     /translation="MAVFVRKLLGIGKLPDDLHAQVEAEGLLYLADYVAVTRRFSGVI
                     PGVRAPHSVASYSGSLAFTSERVLATLSLLPRLAGPTVNVRWDAPQKGAAKVEISSTG
                     LHVDVDVAQVDKKFNGELSLHYKAAIPADVLAGLPRRSLAFDMPPEYVVRAVGVTYRP
                     "
     gene            complement(18792..19148)
                     /locus_tag="VFI55_10590"
     CDS             complement(18792..19148)
                     /locus_tag="VFI55_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074513.1"
                     /translation="MKLSDVLDQLPTDPTARRVYGEPYETADGATVIPVAKIRGRTAG
                     SDPGAPNERGAGGFSSVPVGVFVVHGGQASWVPAVDVNRIALIGVVTGLLAAAIGSLA
                     VLRQPPWPRITITGDR"
     gene            19232..19906
                     /gene="bluB"
                     /locus_tag="VFI55_10595"
     CDS             19232..19906
                     /gene="bluB"
                     /locus_tag="VFI55_10595"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HET7074514.1"
                     /translation="MTDQAFSPQERRAIYRVISERRDMRRFVPGGPVAEDVLARLLQA
                     AHAAPSVGLMQPWRFIRITDESLRRRIHAIVDEERPLTAAALGERAEEFLALKVEGIL
                     ECAELFVVALCDNRGKHVFGRRTLPQMDLASVSCAIQNLWLAARSEGLGMGWVSLFDP
                     QRLAALLAMPADAEPVAILCLGPVPEFPDRPALELDGWAYPRPLSEFVSENQWGQPSP
                     AGRVHP"
     gene            19946..21355
                     /locus_tag="VFI55_10600"
     CDS             19946..21355
                     /locus_tag="VFI55_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET7074515.1"
                     /translation="MTATFAQDAQDAQGAQDSKDSVLMVHWHDLGRYLGAYGHADVSS
                     PRLDRLAAEGILFTRAHATAPLCSPSRGSLFTGRYPQSNGLVGLTHHGWEYRSGVRTL
                     PQMLSESGWYSALFGMQHETSYPRRLGFDEFDVSNSYCEYVVDKTAQWLREAPEQLAG
                     QPFLLTAGFFETHRPYPQDRYQPADSAGVDLPDYLPDTPEIRGDLAGFYGAIAAADAA
                     VGRLLDTLAETGLDASTWVVFFTDHGAAFPRAKSTLYDAGTGIGMIVRPPTNRAMAPR
                     VYDELFSGVDLVPTLLGLLGRDVPADVEGVSHAAALLAPDPQAEPVRNHVYTMKTYHD
                     SFDPIRAVRTKEYSYIENYASRPLLDLPWDIKESPSGVAVAPLVGGPRPKRELYDLRA
                     DPTETDNLLARPDAGVDAVAADLAVRLHDWRQRLDDVIPSEFAGARIAGRYTETYLRI
                     HHTKPTSRSAIAVDRGIEE"
     gene            complement(21371..22156)
                     /locus_tag="VFI55_10605"
     CDS             complement(21371..22156)
                     /locus_tag="VFI55_10605"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HET7074516.1"
                     /translation="MWDPDIYLAFADHRSRPFYDLLSRVGAERARRVVDLGCGPGHLT
                     AYLARRWPEAVIEAIDSSPEMVAAAKERGINAVTADLRDWKPKPDTDVVVSNAALHWV
                     PEHADLLIRWAGELAPGSWIGIQIPGNFETPSHAAVRALARREPYAKIMRDIPFRVGA
                     VVHPPIQYANELLDAGCQVDVWETTYLHQLTGEHPVLEWITGTALVPVRERLDDEGWE
                     QFRQELIPLLDDAYPPRADGTTIFPFRRVFIVAQVGGAPRSGG"
     gene            22258..23448
                     /locus_tag="VFI55_10610"
     CDS             22258..23448
                     /locus_tag="VFI55_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7074517.1"
                     /translation="MTPDADPVGRRVRVPDVPGADVPAGAEGLPSRSALSTRQRMVVA
                     ATAMSDFALRTAIASVLSAAVTPAVIASSTRHGGERDNLRFYADLGSERDPAKSFPAP
                     TELPRVSSRPANRLAARIAGGTVENISFASSFRAVNPAMRTRWDAWKSNNVVHAQHWR
                     HGDGPRPTLCVIHGFMGASYLANGRFFTLPWYYRSGYDVLMYTLPFHGARAEKFSPFS
                     GFGYFAGGLSGFAEAMAQAVHDFRSIIDYLRHTGVQRIALTGISLGGYTSALVASVDD
                     RLDAVIPNCPVVTPATMFEQWFPANKLVRLGLRQSHISRDELAAGLSYHCPLTYRPLV
                     PTNRRMIITGLGDRMATPGQAVLLWEHWDRCALHWFPGSHVLHVSQLDYLRRMTPFLQ
                     QFMF"
     gene            complement(23438..24436)
                     /gene="eccE"
                     /locus_tag="VFI55_10615"
     CDS             complement(23438..24436)
                     /gene="eccE"
                     /locus_tag="VFI55_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccE"
                     /protein_id="HET7074518.1"
                     /translation="MSLHRSIPTPGSGRITLALLAVVPAAMAYPWRSTRDHWVLGIAA
                     AAVILLFGWWRGLHFTTILRRRFAMMGRRPDQTDGATTESATTTTVLLQVGPPVGDSD
                     VLPLGLIARYLDRYGIRADKIRITSRDNASDASRRETWIGLTVSAADNLPALQARSSR
                     IPLHETAQVAARRLADHLREIGWEATPVAPDDVPRLLAPGARESWRGVRRGPSDYIAA
                     YQVNIDDRLPETLAAIRSYPAREVCMALEIAGDATHDTIAAACAFLTEMPPDGAAPLA
                     GLTPQRGNHLAALTALDLLSAQRLDGHTDASAGLLARLVWPTPVAGAHRAPQPART"
     gene            complement(24433..25833)
                     /gene="mycP"
                     /locus_tag="VFI55_10620"
     CDS             complement(24433..25833)
                     /gene="mycP"
                     /locus_tag="VFI55_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion-associated serine protease
                     mycosin"
                     /protein_id="HET7074519.1"
                     /translation="MIRSGSACVTAALVFLQAGVMWACPPALAISPPAVDPGVPPPSG
                     TPGPGQPMEQRGPCSASGVIPGSDPGVLTPGQAMLNLPAAWQFSRGEGQLVAVIDTGV
                     RPGPRLPNVDPGGDFVETTDGLTDCDGHGTLVAGIIAGQPGDDGFSGVAPAARVLSIR
                     TASAKFSPRTPGGDPLLAQASLDVATLGRAIVHAADLGARVINISAITCLPADRTIDQ
                     AALGAAIRYAAVDKDAVIVTAAGNSGQTGSIAGGTSCESNPLTDLSHPDDPRNWAGVK
                     SVSIPSWWQPYVLSTAALTPDGQPSKFSMAGPWVGIAGPGEDIVSVSNRDGGGLANGL
                     PDDHQQMIPLSGTSYAAGYVSGVAALVRSKYPQLTATQVMRRITATAHNGARAPSDVV
                     GAGSVDPVAALTWELPAGDQPGMASTKPVAAPPPPAHKDTTPRTVAFAGTAALALLVA
                     VAGAIVAITRKKETTP"
     gene            complement(25830..>26457)
                     /gene="eccD"
                     /locus_tag="VFI55_10625"
     CDS             complement(25830..>26457)
                     /gene="eccD"
                     /locus_tag="VFI55_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VII secretion integral membrane protein
                     EccD"
                     /protein_id="HET7074520.1"
                     /translation="LSIGCGLIVAALLVTIQAPQLSALWARFPLPVIPAPGDPSPSAP
                     SLRVLEDLPRRVRTADAHQSGFIAAAVLLSVLGSVAVALRPESLSGWGWYVVAATAAA
                     SVLRARVWDSAACKAWLLAQPYLVAGVLLVLYAATGRYPGALGAVLVLVALLSVWVVV
                     ALNPRIASPESYSLPLRRLLGFVAAGLDASLIPVMAYLVGLFSWVLNR"
BASE COUNT         4419 a         9111 c         8775 g         4152 t
ORIGIN      
        1 gccagcatcc tggtggtgac tcataacggg ttctgcgccg acgggcggga ccggccctgg
       61 tggcagctac cgattcaccg gcagttgctc atccgcacgg tcgtggtgcc ggcggggcgc
      121 atcccaccgc cgacgcgggt cggaccttgg ctcgaacgaa cctggacaca agtcgatgcc
      181 tgggtcgccg ctcatgtcga gcagtcctga tgtcttgcga ccaccgcacc ctgtgtgacg
      241 cgaccagacg cgaccgatga gaattggcag accatggagg caaacggcta cttcgagaag
      301 tggctggaaa acgtcgagcg tcggggcgtc ccttcgggcg tcgtcatcga gatcgctcga
      361 cggcacgcca tatccccacg gagctttgag atcctcgaga cgatggagga gatcaacgac
      421 ccccgcgaca agtcctactt cctcatcccg cccggcgccg gggggcagcg cgcgcgccac
      481 gcgacgctga tgacgtacat cgtcaacgcg ggcaccgact acggcagggc gggaaggcgg
      541 gcagactttc ccgagacacc ctactgcgcc gccgaggtgg cgcggatcat caaccggcag
      601 aaggcaaacg gctggagtta cacccgagac gtcgggtttg tcgatcgcaa cgggggacgt
      661 ctcgtcacca ccccgaacgg catgctgatg ggcctgggcg gcaactggat ccaaaggcag
      721 ttcagccagc ggggcggcac cacgtggggc gacatcttca tggtcaacat gggcaaggtc
      781 gccgaccccg cccagcagct tcgcgagatc gtgggctccg ggcatgcctg gcacaccctc
      841 gatttggacc gggtactgca ccacgaggag cgacacagcc ggcaatgggc ggccaggggc
      901 tatccgggca tgatccggga ctacggccgg gagctgttcc gcgagttggc ctttggcaag
      961 acgaacaggc tcgaagaaga cgccggcctg tccgacggag gctacagggc gtcaatcccg
     1021 ttgtagagca ccatcgcgcc gatcaccacc aggatggccg ccatcagcgc ggagttgttc
     1081 ttttccatcc agtctttgag ccgtgccatc gtgtcgtcga gccggtggcc ggcggccgcg
     1141 taggccagta tcgggatggc aaccgttgat gcggcgatgg caacgaagaa cgcggcggct
     1201 accgagtcat cggccacgtc cagcccactt ccgccgatcg ccaatccggc cggaacgcag
     1261 atgagcacca cgtcgggccg tatcgccacc agcaccgccc ccgtcagccc cgcacgcgcc
     1321 ggggtgatgg tggcgaacgt acgcatccac gccggcgact cggtgttgcg atgccgggtg
     1381 aaccatcggt agaaaccgaa cagaatcagg gccgatccga ggaccacgcg cagccacgac
     1441 gcccacgtcg gcggggactt gtgcaggccg cccagcaggc ccgagccgac gacggagagc
     1501 gccgtcagcg cggccaggcc cagcacccaa cccgccagaa aagcgaggcc gctcggcctc
     1561 ggccgcggcg cctgcaacac cagcaccgcc gggatgatcg tgagcggtga gagcgcgatg
     1621 accagcccga gaggaatgag cgcgcccagt acagaacccc aacttcctgc cacgagcagc
     1681 aatcttcgca ttcgcagcga ctcgatgccc acaaagcgcg ccgcgaacgg gcccggaaat
     1741 cccgatgtct acagcgcgtg aattccgttg tagagcacaa gcaaaccgat cagcaccaaa
     1801 atggccgccg tcagcccggc gtggttttgt tccatccaga ctttgagtcg ttccagcgcg
     1861 tcgtcgaggc ggtcaccggc ggcgacgaag gccaagatcg ggatggcaac cgttgacgcg
     1921 gagacggcga cgaagaccgc gccggctatc caggcgcccg cgacgctgag gccgccggtg
     1981 ccgatggcca ggccggccgc cgcggacagg accagcacct cgggtctcaa cggcgccagt
     2041 gccaccccgg tgagtcccgc gcgcaccggt gtgagcttgg aaaacgaacg catccagccc
     2101 ggagtcttgg tttgacgatg ccgggtcagc cagctgaaca ccccgaacgc gatcagcacc
     2161 agccccagga cgacgcgcat ccacgatgcc caggtcggcg gcgccttgcc cagatcgccg
     2221 agcaggtcgg agaggccgac gaacaccccg gtgaggccga ccagacccag cacccagcct
     2281 gcgaggaagg cgaggctggc cggccggggc cggggcgtat ggaggacaag caccgccgga
     2341 atgaccgtga tcggtgagag cgcgatgacc agcccgagcg gaatgagctt ggtgagcact
     2401 gaaccccagt tgtcggccac gggcagcaat catcccattg ccgtggtagc cgaccacggt
     2461 cagccaccgc agttctcgag ctagtggcct ttccggcgca acgtccaggc cggtacccag
     2521 tgcgtcacgg ttctccgctt ggagccgtac tcgatcgggt aggtcaccaa cccccaccag
     2581 tcgccctgct cgcagattac ccagcagctg agccgcccct cgacgacctt gtgcagctgc
     2641 aggccgttgg gctgatacct gcccgagcgg tgcggctcgc gcgggaagag caccgtcagg
     2701 tcgaccagca ccgccaccgg cgggcgcacc agccggaacg ggctgcggac cggttggccg
     2761 ttgtagccga tcgacgcgag gtcgcccatt gatcgatcat acgttcgaat ccctggtcga
     2821 ttcagcccgt cacgccggaa ggcccaggtc gggcttctgg caacatagcg gtggtgttca
     2881 gcgcgtccgc gatccgcccg gtggctcggt gagcgtcgcg gatacccgcg aggcagggca
     2941 ccgccggggc gttgtgccgg cagcgccgtc gccggcgctc gacccgctga tcgttggaat
     3001 cctggccgcc gcaataagta tgggcggcgc cgcgcgcccg tctttctggt acgacgaggc
     3061 cgcgaccatt tcggcctcct acagtcgctc gctcggtcag ctgtggcaca tgttgggcaa
     3121 cgtcgacgcc gtgcacggcc tgtactacgc gttcatgcac ggctggttcc aggtcttccc
     3181 accctcggag ttctggtccc gcgtgcccag cgggctggcc gtcggagccg ccgcggccgg
     3241 agtcgtcgtg ctggccaggc agttctcgtc gcgcaccgtc gcggtggcct cgggagcttt
     3301 ctgcgcgatc ctgccccgga cgacgtgggc gggcatcgag gctcgcccgt ctgcgctttc
     3361 gatgatggcc gcggtctggc tgacggtggt gctggttggc gccacccgca ggaacacggc
     3421 ctggtcatgg ctgtcgtacg gcatcgccct ggccgcgtcg gttctcctcg acgcgtacct
     3481 cgcgctggtg gtgttggcgc acctgagctt catctgcgcc taccagggga cgcgaaaagt
     3541 gctgacgggc ttcgttgtta cgtcgatctt gacggtttgc acgttaacgc cgtttctgct
     3601 cacggtcgcc gggcaggcgc accagatcag ttgggtcgca ccgatcgggc atcggacgat
     3661 cgaagacgtg gcgatgcagc aatatttcga gagaagcccg ccgttcgcga tcctgtcggc
     3721 attggtgatc gcggcggcta ttgtcttgtg gctcggcgca tccgcgcgac tcgcggacgg
     3781 ggatcgccaa ctgctgagca ttgcggtcgc atggctggtg ataccgacgg ccctgatcgt
     3841 catctggtcg gcgctggctc atccgatcta cacgccgcgc tacttgagct tcaccgcacc
     3901 ggccatggct ctggtcctgg gcgtctgcgc cgctgcggtg ggcgtcaagc cctggggaac
     3961 ggcggccctg gtcggcctgt tcgccatcgc tgccgctccg aattatgttc tggcacagcg
     4021 taatcagtat gcaaaatacg gaatggacta cagtcaggtg gccgatctga tcgccgtgaa
     4081 ggcggcgccc ggtgactgcc tgctggtgaa cgacacggtg acgttcatgc cggcccccat
     4141 gcgcccgttg atggccgcgc gccccgacgc atatcggaag ctggtggacc tcaccctgtg
     4201 gcaacgggcg acggaccgca acgacgtctt cgataccaat ctgatcccgg aggtcgtcgc
     4261 ccagccgttg agtcactgcc gcgtcgtgtg gatcattacc caggccgacg cgtccgtgcc
     4321 cgcccacgag cagggctcag cgctcccgcc cggtccgcgc tacggaccga cccccgcgtt
     4381 cgcggttccg catgacctgg gcttccggct tgtcgaacgc tggcaattca acctggttca
     4441 ggtcatcaag gccgtgcgat aacgctcacc agatgcgcag atagtcgacg agcatggcgc
     4501 acgggaaggt gccctgggcc ggatcgccgg cgcccacccc gccgacggcc agcgtgaaca
     4561 tgggcgtcat ccagtagccg ggggcgttga acggccaccg catgtcgtcg ggggcgccgc
     4621 cgtgcacgtg gatgggtttg ttgggaacgc tgaagtacgg tgccccgtcc cgcgcgaagt
     4681 ggaatccgtc ctcagcccaa tgcatctgcc aggtgtgcca accgccgtcc accagaccgg
     4741 gaatcgattt cccctcccag gtttttccgt tggatgccgc gtgcaccgtc gttcccgggg
     4801 cccagtcgcc gttgccgtac cactcgaaaa tgtcgacctc gccgtccggc aggggatcct
     4861 cgttgacggc ccagaacgac ggccacaggc cggggaacaa gcagtccagc ttgatccgcg
     4921 cttcccaggt ttggttgatc atgctgcgaa agttgccgcg cagtttgccg ctgtagtaat
     4981 tgcccatttc ctgggtggcg cagagcacga gattggagtt gccgtcgagg aacacgttgc
     5041 ggcgatcgtc ccggtatatc ccctcgaccg gcgggaacac gtcgtcctgc caggtctgaa
     5101 tcgtccactt ggccggatcg gggggtgcgc ccgccgggcc gtcgaactcg tcggcgaaaa
     5161 tgtagggccc gccggccccc gccgccggcg cgccgggcgc cgccggcgct gtcggactgg
     5221 cgtgggcctg cgttgcgccg accgcggccg ccgccgccag catgccgatc cccgtggtca
     5281 acaacatgct gcgacgatcc atctgcacta ccaccaatcg ttcaaaagcc gggatgcccg
     5341 gcctcgcctg atttccccta ggcaactttc gcaaacggtg ttatagaacc acgccgaggg
     5401 gcaggcatgc atctcgccag accgtgcgac cccgtcaatt tcgacgggat aaccgctgtt
     5461 gggtgccgga tccggccgcc accaccgccc gcgtcatgcc aggtcgggga cctcagagat
     5521 tcgcacagtc ctccccgatc cgggttcccg ccgcatagag cagttgcttg aggcggcgtt
     5581 gttcgtcggc ggtcagccgg gtcaagatcc gttggtcggc aacctgcacg gcgacgtccg
     5641 cacgcttgag cagggccttg ccccggcggg tgagccgggc cggcatcgcc cggcccgccg
     5701 ggctcgacgc gggacgggtc agcgcgccac gttcctccag cccgtgcagc acctggttca
     5761 tcgcctgggc ggagacgttg gtgccgcggg ccagttcggc gctggtgagc ccgggatggt
     5821 tcgacaagat ccgcaagcac acgaattccg gcaggctcag ctccagcggc ttgagctccg
     5881 cggcaacctg gggccgcagc gccgccatca cgcggtacag caaaaacccc agcggctcct
     5941 cgtcggaatg actcatgccc cacatgctga cacgggagaa ccggtttccc gcaacccacc
     6001 atcaatttac ttgacatcgt cggagccagt ggtatcaagg agcttgatag tccgatatgg
     6061 aaaggaacga cagcaatgtc tggtggattt ttcggtggct tcggtggacc cggcttcggc
     6121 gggcacgcct gcggcggctc tgacggtccc ggctttggcg gcttcggcgg cggctggggc
     6181 ggaaaaggct ggggcggtcc cggtttcgaa ggcggtttcg gcggcggccc tggctccggg
     6241 ccaggtttcg gccccggccg tcgccatttt ctcaagcgtg ccgcgttcgt gacggcggcg
     6301 ctgctgctcg acgggccggc ggacgccgcg cagatcgtgc agcgggtatc cgacgcgacc
     6361 cagggcgcat tcacgcctcc gcaggacgtg gcagagcttg ccatcgggct gctggccggc
     6421 cgcggcgtgg tcacggttga caacggcgtg gcgacgctga ccgaactggg caagaacctg
     6481 ctggcctggc gtggcatcag cagcgagacg gcgcacgcgt tcctggggcg ggcggccaag
     6541 ttcggcgacg tcttcaagat ccgcaggacc ctcttcgaaa tcgccgggct ggctcgcacc
     6601 atcgtctgga cgggcaccga cgagcaaaag gagcagctcg ccgagacccg gaccaaggtc
     6661 ctggaagccc tgaccgaggc gaagaagacg ctgcaccgcg tcctcggcga ggactagacc
     6721 ctcagccgcg tgcggtttcg ctgagcctga ccagcatctt gccgatgttg gccccggtga
     6781 acagcccgtt gagggcgtcg acgcacgact cgacgccctc aaacacggtt tggcggtgcc
     6841 tgatccggcc ctcgttctcc cattggcgaa gcgcggcgaa ggcttcgccg aagcggcccc
     6901 actcgtcgag tgcgttgaat ccctgcataa gcgcggtctt cgccaacagg ttcacgtagt
     6961 tggccggtcc ggggtgctct ccgctcaggt agctggagat cactccgcag agcaccaccc
     7021 gcgctttggt ggccaggcgg cccaacaccg cattcaggat cggcccgccg acgttgtcga
     7081 agtagacgtt gacccggcgg gggcaatgct ccttgagcgc ccccgccagg tcgtcgttct
     7141 tgtagtcgat gcacgcgtcg aatccgaagt cctgcaccac cgcccggcat ttctccggcc
     7201 cgcccgcaat gcccacgacc cgggcgccgg cgatcttggc gatctgtccg gccaccgacc
     7261 cggtggcgcc cgcggcggcc gagaccacca ccgtctcccc gggctggggc cggccgatgt
     7321 ccatcatccc gaagtaggcg gtggccccgg tggggccgta gaccgacatc acggccagtt
     7381 ggtcgaccgc ggcccggccc gggatgggtg tactgaacac gtcgtcccgg atgatcacgt
     7441 actcctggaa accggtcagg gcggtgacga cgtcaccgat ttcgtatgcg tcacaccgcg
     7501 aggccacgac ctcaccgatc ccggccgcgc ggatgacctc gcccagctgc accggcggca
     7561 ggtaacccgg ttggtcgttc agccaggtgc ggacagcggc gtccagcccg acgtaggtgg
     7621 tccggaccag cgcctcacct gccgcgacct gcggtgccgg cgtggtgacc agctcggtgt
     7681 cgccgggctc aagcagcccg gtggggcggc ggcgcaacac gatctggcgg ttgggcagat
     7741 cgggcatggg gcgaaaacta cccacgggct gaaactaccc aatggggccg aaactaccta
     7801 ttgggcgcga ttggatggga gcatctgtgc atgaacccgc aggatgaccc ggagaaacgg
     7861 atccgcgaac tcgagcgccc cctggccgac gcggcgcgcg cctccgaacc gggggaagcg
     7921 cacccgcccg gcggttacgc gtatccgccc gccccggcgg gcccgccgcc cccgccgccg
     7981 cccccggccc cgtggacgta tggcggcgcg tattccgcgc cgccatcgaa accgccgtcg
     8041 agcaaccgca tgtggtggat cctcggcacg gtcatcgtca tcggtgcgct cgttctcgcg
     8101 ggggccatcg ccgccttcgt cgcaaaccag ctgtccagtg tccgatcgct catcacgagc
     8161 ccaccgaccg tctcccggac gttcgttcca ccggcgatca ccccgaccag cccgggcccg
     8221 tcggcaagtg ggacgaacac acccagccca tcgacgtcac cgaccccgcc gccgggtggc
     8281 gagctcagtg tctcgggtat caacgcgaat cgaacgatcg cctgcaacga cagcatcgtc
     8341 gacgtcagcg gggtttccaa caccgtggtg atcaccggcc attgcgcaag cgtcacggtg
     8401 tccggcgtgc agaactcggt caccgtcgat gccgtggaca gcatcgacgc gtccggtttc
     8461 aacaacaagg tcatctacca ctcgggcacg cccaaggtca gcaacgccgg cgattcgaac
     8521 gtggtgcaac agggctgatc gccaacggcg tcaggccatc gcttcggcct gcgccagata
     8581 tccctcgaag tcggcctcag ccgccttggc ccggaggcgc gtcacgaact gccgataacc
     8641 gggctcatcg ccgtgcgcgc gagccagcag cgcccgcagc cgcagtacgg gaagctcgtg
     8701 taggacaaac cccggctccg tcggtaccgc cgccagtctg tcgatcgccc ggcgcgcgtg
     8761 catgacgtcc gcacccgagc cgcgggacag cagggcctcg accagcgctg tggtggcggg
     8821 cccgcggaag atcatctctc cggtgtcgaa ctgctcgacg acaaccttgg tggccaaatc
     8881 gatcgcgccg tcgagatcgc ccgacctcgc gcgctcgcgg gcgatctcga tatcggccat
     8941 tcggcgcagc gtcacgatga gctgttcgtc gacgatcatc tcgcgcgccc tggccaggaa
     9001 ttcgaggccg gccgcagtgt cctcggagtc ggcgcggatc aacgtggtgg cccgattcag
     9061 cagcgcatac gcgaccgcgg tgttattcgc ggaccgctgc gcaatttcca gagtctccgc
     9121 ggcctgggcc acgtcgtggg gatcgggcag caccgcgcca ttctgaatcg cggccgcgta
     9181 cctgtagagc tcaacaaaag accgggtggt tttgccgcca accgatcggg ccaagaccag
     9241 tccttcgtac aggtcgtttc gccagccgcg ccgtcccagg gacattccgg ccactccctt
     9301 gaccgcgatc gcccaggcaa gcggagatcc gaccaccaag ctgcccatgg tgggatcacc
     9361 gtcagcgaca tcgatgatgc gttgggccaa ccgcatgctc tcgctcgctt caccggcttc
     9421 ccacttggct tgggcggcgg catagaagag cccggccgtc atctccggat cgccgatcga
     9481 ctcgacgagg gtggcgaact ctgatgccat ggcggccgct tggcaatgat gggcgttgaa
     9541 cgtgagcgtg gtgaggtgtc ccgccattcc gatggccagt gacctcttgt cgcccgccgc
     9601 ggtcgtgagc tcgcgcaatt cgtcgaaacc ggtgtcggca ggtgtgccgc ccacctgaaa
     9661 agcgctgcca cacaacagcg ctcgcggagc gatccgctta tcgagccgat ccgggtcgtc
     9721 ggcgggcaac cgatcggcga ctcgaagcgc ctgttgccaa ctcttcctgg ccgccgggat
     9781 atcacgcgcg ccgtaccaag tcgccgcccg catgtgccag tcgtatgcat ctcgcagatc
     9841 accggcggcc gcgtactggg tggcgacgat ggcggcctcc cttccggtga acccggcttg
     9901 cgttcgttgc atcaccgcgg ccacccggcg atgcagctgc gagcgccccg ctttcaactg
     9961 tgattcgtag gccacctgct gaataagcgg atggcagaac gcgtatgtcg tgcgcggcgt
    10021 cgagccgacc tgttcgacga gctgcgcctc gatcaacggt cccacatcga ccgaatccaa
    10081 cagcgactcc agcaattcaa gatcgaactg tgcgccgatg acggctgccg cgctcaacgt
    10141 gcgcttcgcc gtaccggtga gcctgtcgat gcgggctccg atgatcgcct gcagactggc
    10201 aggcacatgg atatcgcgca cctcccggat gcagacgtag gcacccggtt cgccctcgag
    10261 ctcgccccgc tcggtcaggt cgcgcacgat ctcctcggcg gcgaacggga caccggccgc
    10321 ccggtcggcg atcaccgtcg acagcccgcg taccgacggg tcctcgccca acagcccctt
    10381 gatcaactca gcgatctgtg aatcactcag cggcgcaagc gtaatgggat gcgccccggg
    10441 gaggcgcgac aaagcccccg aatattgggg ccggtagatg acgagcaacg tcgcccgcat
    10501 tctgggcacg gccgccgcga agtcggccag cagcttctcg ctgacgccgt cgatccactg
    10561 agcgtcctcc acgatgtaaa tcgccggtgc cgggcgtacc tgcgaaacac tgttgatgag
    10621 gtcgacaaga cgccggcgcc gagcgtccgg attgacgtcg ggacacggac caccgcagat
    10681 gccgagcaga tcgtcgagca gcagcagatc ttcagcaccc gcgtcgggga tctggctgcg
    10741 aacccgtgcc cgggcttcct ccgcggtgaa accggcgatg ccgaaaatcg cgcgcaacag
    10801 ccgggcgatc acatgcaacg ggacgtcacg agtgtgggac tcgcagtagg tgacgaacac
    10861 ctcgtagccg cgactggtcg ccgttaccac cgactcgcgg accaacctgg tctttccgac
    10921 acccgggcgg ccggtcaccg tgacaatggt tccttcgccc cgacaagccc ggtccagtaa
    10981 ttccgcgacg gcctgcttct ccgatttgcg gccgaccagc cgagactgct gacgggcgct
    11041 gtgacgatcc tcgagatcga cgcccagcag gcggcgcgcc ggcaccgccg tggcgaaacc
    11101 tttgatgtgc accgtctcgc gctcacccag gaccaccctg tcctcgacga gccgtgccgt
    11161 cgactcgctg agcatgacac cgcccggggg tgccaccgac tccatccgct gggccatgcc
    11221 cacctgctca ccgaccgcgg tgtagccgag gtgggtcgcg ccgacctcgc cggcgatcac
    11281 ccgaccggaa ttcaggccga cgcgtatctg caaatcgatc ccgtcgcggc gcctcacctc
    11341 gaccgccact tgcgcagcga cctgctgcag ctccagagcg gccaggcagg cacgaaatgc
    11401 gtggttctcc aacgtgattg gcgcgccgaa gagcgccatg atcccgtcgc cggtgaactt
    11461 gtccacggtg ccgccgaagc gctgcaccac ggccatcgac tggctgacca gatcggtcac
    11521 cagctcgcgc agccgttcgg gaccgagagc cgcggcgatg tccatcgagc gcaccatgtc
    11581 ggcgaacagg acggtcacct gcttgtattc ggccggatca acgaccagcg tcagcagcga
    11641 gccgcaggca tcgcagtacc gcgcaccctc acgcggctcc gtaccgcaca catggcacgc
    11701 caacggtgtt gccgtcacac tcgacccttc cgagacccct ccggcggagt tcccgtctag
    11761 tgggaacgca cggcaacggg ttggttatgc cggctcttcg gaatcaccgt cgagaacgct
    11821 gaccgcgatg ccgtagggca ggaagcgcac cttgcgcatc gggtcgacat ttgtcttgtt
    11881 ggcccgcagc gcgtcgagct cggtcgcata gacctcctcg acgtgagcac cgccatcagc
    11941 gttcacgctg tagatcgccc acaccccgtc accggcctcg ccggcggtct cctgctcggg
    12001 gcggggtcgc cgcgacaggt catcgaagaa cgccgaccag cttgtccggc cgccgggacc
    12061 gacgacaagc ttgccgatcc gctcgaacac gtcgcgcagt ccctcgctgc cctccctgat
    12121 catccgatcg aattcctcgg gatcaaatcc gaacgcaccg tattcgccca cgcagacctc
    12181 ctttgcttcg cagcgctatt tgcccagtgt gcgcccactc gacggcaccg tgctaccaac
    12241 gctgtcgcaa gcctcgctga gcccgcccga tcagtatccc ggacccagga acgggttctg
    12301 cgagctcccc gggtactgcg agttccccgg gtactgcgag ttccccggat actgcggata
    12361 ctgcggggcc tggggcggct gaggagcctg gttctgcgga accgggacgg gtatcggcac
    12421 cggcagcaac gggacgtgta tccactccgt cgtcatcggc accgtcgtcg tgggggtggg
    12481 cggcggagtc gacgtcggcg gcgggggcgg cggaggggtc gtcgtcatca ccgtcgtcgg
    12541 cggcggagga ggcggctgcg tcgttgtggg cgggggcggc ggaggacgcc tggtggtgac
    12601 gaccggcggc ggcgtcgtca cgatcacccg cggcggcggt ggtggcggcg gcggaggcgg
    12661 tggtggcggc ggaggcggcg gctcgggggt ggggaccggc tcggcgctcg gcgacggcac
    12721 aggcgacggg ggcggtggtg gtggcggtgg caccgggctg ggggccaggg ggctggccgg
    12781 cggcgccggc accggcacga cggtgggcgc cggaggggct tcgcggtttt ctatgcctgc
    12841 caacgtgtag gccacgccac cgatcgcggt catcgcgacc accgcgcaca tcccgatgat
    12901 caactgcgac agccggagcc gccgccacgg tttttccttg ggtggctcga tcaagttcag
    12961 gcgcatcgac cagccggccg caccgtcttc gtcgaatgcg gccggggtgt agggcacccg
    13021 cacgtcgccc gggtattcgg tttgcgacca ggccaattcg cgatccgtca gcgcctcgcc
    13081 gtcgatcacc aggacgtcgc tggcaggcag ctggaccacg tcgctggcgg cgcccgccgt
    13141 gagcaggccg atggacgtgc gcgtccggat atccagctgt tcgccacggg aggccagcag
    13201 caaagcgccg gcggccgccg cgaaggcagg ctgcgacggg gccaccacgg gtctgcggga
    13261 atgcaccgaa agacgctcgg tgacaagcgg aatactggcg cccccgccga cggtgaccac
    13321 cgccgacaga tccgcccagc tcttcttgtg gcgggccagc atctcgtcga acgcatagat
    13381 gaagccggac agccgatcct gaatcaggtc acccaactcg tcgcgagtca gcttcatgct
    13441 gcacctgcgc ccgcccagct cggcggcgaa ttcgactacc gtttcggtgg ataaccgctc
    13501 cttggccgtg cggcactgct ccctgagcat ccccagttgg ccgacggcgg cggtgctgtc
    13561 cgggtcgata ccactgccat gcccaagctc ctcgaaggcc ttgagcacca aggcctggtc
    13621 gatctcgtcg ccggagaagg ccgtgtaacg cagtgtgggg ctgacgagct cgaaatctcc
    13681 cgtcaggctc accaaggtgg ccgacgtacc cgtgccaccg aaatcgagca gcccgatcac
    13741 gccggttgcc gcgaagcaca aatccgcgtt cgccgcggtc aacgaggcga ttgcgtccga
    13801 gaccagccgc ggcgccatac cgctgcggac gaagcccaga tgcgtccgta atgcgttacg
    13861 caacgcctgc acggtcccgg gcttccagta ggcgggcacc gcgatggaga tctccgatgc
    13921 ggctgcgtcg gccccggcgg ccaccaccat cgcgtccagg gcctccacca tcaggaggtc
    13981 cgggtcatgg gcggatccgt cgggcgacac cagcgccacg gaatcaccga tgcgctccac
    14041 gaagccacgc atcagcatgc cggcctcgat caggctcgga tcttcgcagg acaagccgat
    14101 tttcggcgcg cagtgcggat acagggtcag cactgcgcgg cgcgagaccg gcggagttcc
    14161 gttacacgca gcgaccaggt tcgtggtccc gatcgacaac cccagtgggt cgtacataga
    14221 gcgaatacct tcgtctatag aggtcggtgg ccagcgttaa ccatagtcgc ggacacgctg
    14281 aaagccgatg ggttgcaatg tcattggtac tcgctcgatg cgattgcgtc accgaagcgg
    14341 tatcgaaaac cccttgacag acgcgttgac gtcacaccac ggtcagcggc agcgaacgcc
    14401 gcggctggaa ctggaatgac gatccgccgc tgaatttggt caccgacacc tctggcagtt
    14461 cggccgtcgg ggcatcggtg tgcaccaatt ccgcggtcca gtcggtggcc gttccgctaa
    14521 ctcgaaccgc cacgcgcggg tccaccgcgg tggcgatcgc ctgcagcggc tgcacggatt
    14581 cgggtgagca cagcgatatc caggcgccgt catcgtgggc gggcgagcgg cgcaccgtga
    14641 gccggttgcc ggccgtttct gccgcgacgt agccggcggg gttcagcagc ggatgcaact
    14701 cgagcacccg tagcgttccg tcaatcgagc cgggcaaccg cagcgcacgg tgaatccgct
    14761 cggcggccag ccccgccagg ccgatcaagc cgcgggtgcc caccgagacg gcctcgtcct
    14821 cggttgcggc gcgtgcgcgc accgcgatcg cgaacgacag atagagcagg tgcatctgca
    14881 gacagacctc gtcggccatg cggaccagcg ccgagtgcga gaacgcgccg aagtcgagat
    14941 cggacagcaa cggacccgaa taatccggcc gcccttgccc agagcgctcg atcggcccga
    15001 gttcccatgt cgcagcgctg gtttggcgaa cgatgtccag cgccggaatg ctcgttgccg
    15061 ctgggtgaga ctcgtcgatg acgacggtcc aggcgcagtg cggatgccta tccgaaggaa
    15121 cgcgcggcgg ccggtggatg ggacgcatct gggcgcgcgc gttggttgcc accgcggtgg
    15181 cgtcgaaggt cgggtcctcg atggtgtgac acatcccgaa gacgtactcg tcgcccatcg
    15241 gctccacgtc gagcagcgcg ccgcaatggt cgagctggaa ttcgccgtgc caccggtcgt
    15301 gcacggtgta gcggaaatcc atgaattgcg gtggggcgcc gatgtcgagc tgcaggccct
    15361 tgaagatggt gggcacatcg acgccctcgt agttcagcgc ccgctgcatg cgcgtggtgt
    15421 agatcgggct ggcacccgcc ccctcctaga tggcgatctg cgtcatctcc tcgcggccga
    15481 acgatgcgat gcaccaagcc atcccggaac ggtcgatcat gtgcccgatc aacagcaatt
    15541 cgggaaccag gacggcaagc tccgggcggg acaaccgctc gtatcgggat gggctcaccg
    15601 cccaaaaata agtctgacta tgttataaag tcaacaatgt ccattttcgc aggccagaag
    15661 caatcggtcg gccctacccg ccgcaccagt gcgccacgca aacgcggcga tgacacccgc
    15721 acgaaaatca tcgacgagac ggtccgctgc atcatcgagg aagggttcgc cgccgccacc
    15781 gccaagcacg ttgccgaacg cgccggcgtg acgtggggag tcatccagta tcacttcggc
    15841 gaccgcaccg gcatgctgat ggccgtcgtc gacgacggtg tggccaggct ggtcgacagc
    15901 ctgtcgtcgg ccgacgtcag cgagcttccg ctgcgcgagc gcatcgaggt cgtcgtcgac
    15961 accgcatgga gctgctacag cagtccaacg tcgttggccg ccttcgaaat cctgcgcgcg
    16021 acccgcggcg gccccggcgc gtcctcgcgg cgccatctgc tcgaaatgaa ctccgcgatc
    16081 ggccagctcg gccggctgat caccgaggac ccggccaatg ccggtgtcgc cgaggtgatc
    16141 tgggcggcgc tgcggggtgt ggtgctggcg cagatgatca ccggaaccga gatcgactgg
    16201 cactgcgaac gtcgggccct gatcgacatg gtcagcctct atctgcaacg gccgggcggc
    16261 ctccccgcgg ctccacccga tgtgctgcaa tgacgtcatg agcccccgcc attatggtcc
    16321 aggcgcagcc gagctggccg acatcgaagc catcaagcag gtcaaatacc gctacctgcg
    16381 cgcactggac accaagcact gggacgactt cgccgacacg ctggcccacg acgtgaaggc
    16441 cgactacggc ccgtcgatcg gcgaggagct gcacttcacc agccgcaccg acctggtgga
    16501 ctacatgcgt attgcgcttg gcccgaacgt catcaccgag caccgggtga cccatccgga
    16561 aatcatcgtc gccgacgaca ccgcatcggg cagctggtat ttgcaagacc gggtgatcgt
    16621 ccccgaattc gaattcatgc tgatcggcgc ggcgttctac cgcgacacct accggcgcac
    16681 cgaagccggc tggaagatca gcagcaccgg ctacgacagg acctacgatg ccacaatgtc
    16741 attggcgggc atgaacttca agatcaagcc gggccgcgcg ttggcctgat cgccgcggcc
    16801 gcgcacctcg aatgccgacg gtgcgacaga ccgaaatccc ttcgtacagt ccagcgtcgg
    16861 gaccgacggc tacttcgtaa tcgcgatcac cgcgccgggc cgtagccacc ggatgacttg
    16921 caccagtgtg gcgtcgtcga tggccacgca gccctcggtg ggaccgccgt cggtggtgtg
    16981 gaaaaagaac gcggcaccgc cgccgggaac cttgttcttg ttgaccccca tcacgaccgc
    17041 gtgcttgtac tgcggaatct gcaggttctc actgtcggcg gtgttgaacg ggcactggga
    17101 cttctggcat acctgcatgc tgttgaaagt ggggctatgg tcgtcgccac tccaccaatg
    17161 gttgggcccg acctgcgtat agggcagtcc cccaccggga ttcggcgccg tgccgaaggc
    17221 cgagtcgagg gtgtacacgc ccatcggggt cgccggaacc tcgcttctgg cctgtggcgc
    17281 catgcccgcc gacccgacgt gggtgggaat gcccgcggcg agtgcctgcc acccggccgt
    17341 ggtgcgctgg tagatatcca tcttcgcgtt cgaaccgccg gtgctgacga ccgaaaccac
    17401 ttgagtggca ttgccaacgg agttcgcgaa ccacggactg gccgcgccac tcaccggtgc
    17461 cagcgcgaca tacaggcagc tcaggcacac cgcagcgcac agcgaagcta gcagtcggcg
    17521 catgggtcca atcgtcgggt ggccccaacc ccagggtcaa gtaaaacttg ggactcagtt
    17581 gggtcaccgg gctgtgatca ggggtcaggc ggacctgggg agcgccaccc gcgcggtcga
    17641 aatatcaaca ggcacaacag aaaatacagg tatcccagcg accagccggc catcacgtcg
    17701 gacgggtagt gcacattcaa tgcgacgcgg gcgactccga ccaacaacac gcagaacgcc
    17761 gccaccgcga tcgcgacgcg gcgcatccat cgggtcatca tcggcagcag gaaggtcagc
    17821 agggccagca cggcggacgt cgcctcgagc gcgtgccccg aaggaaacga cgtcgaaggc
    17881 gcgggcacca gcatggtcgg cggtctcggg cggttcacca ggcccttggc cgccgtcgtg
    17941 accaacccgc tcagcggccc gcaggtgagc agcagcagtg ccgcccgcac gttgcgcttc
    18001 gccagcgcga ccaccgcggt cgccaggccg accacccgta ccggaaccgg acccagcgca
    18061 aacgagaccc cgtcccaaaa gcgcacccag cccgggtgct tgacggcgat gtcgtgcgcg
    18121 gcgttcaaca gcgaccaatc gacgcgatgc agccaacacc aatcctgccg gtagcccaac
    18181 cacatggccg catacaccag ggcggccgtc agagcagcca tcagagcagc cactagagca
    18241 gtcgaagccg cggccggttc ggtccagcgt cgggtcatca gtcaacggat accagcacgg
    18301 tgcgcggaac ggtcatactg gcgtcatggc ggtctttgtc cgaaagttgc tcggcatcgg
    18361 caagctaccc gatgatctgc acgcccaggt cgaggcggag ggccttctct atcttgccga
    18421 ctacgtcgcg gtgacccggc gattcagtgg cgtcatcccc ggcgtgcggg cgccgcacag
    18481 cgtcgccagt tactccggtt cgctggcgtt cacctcggag cgggtgctcg cgacgctgtc
    18541 tttgctgccc aggctggccg ggccgacggt caacgtgcgt tgggacgcgc cgcagaaggg
    18601 cgcggcgaag gtcgagattt cgtcgaccgg tctgcacgtc gacgtcgatg tcgcccaggt
    18661 cgacaagaaa ttcaacggcg agttgtcgct gcactacaaa gccgccatcc cggccgacgt
    18721 gctcgccggc ctgccgcggc gatcgctggc cttcgacatg ccgcccgaat acgtggtccg
    18781 ggcggtcggc gtcacctatc gcccgtgatc gtaattcgtg gccagggcgg ctgccggagc
    18841 accgccaggc tgccgatcgc ggccgccagc aggccggtga cgacgccgat cagcgcgatg
    18901 cggttcacat cgacggcggg cacccaggac gcctggcccc cgtgcacaac gaacaccccg
    18961 accggcaccg agctgaagcc gcccgcgccc cgttcgttgg gcgcgccggg gtcggagccg
    19021 gccgtccgcc cgcggatttt ggcgacggga atcacggtgg cgccgtcggc ggtctcatag
    19081 ggctcgccgt acacgcggcg ggcggtgggg tcggtgggca gctggtccag cacgtcggaa
    19141 agcttcacgg aacagtcccc tctgcggtgt tcaaagcggc gtgacgaaaa gacatcattc
    19201 acgctaaccc gcttttttct acgatggcgg ggtgaccgat caggcgttca gtccccagga
    19261 gcggcgcgct atctatcggg tgatctccga acggcgcgac atgcgccgct tcgtgcccgg
    19321 cggcccggtg gcggaggacg tgttggcgcg cttgctgcaa gccgcgcacg cggcccccag
    19381 cgtgggcctg atgcagccgt ggcggttcat ccggatcacc gatgagtcgc tgcgccggcg
    19441 cattcacgcg atcgtcgacg aggagcgccc gctgaccgcg gcggccctgg gagagcgggc
    19501 cgaggaattt ctggcgttga aggtcgaggg cattctcgag tgcgccgagc tcttcgtggt
    19561 ggcgctgtgc gacaaccgcg gcaagcacgt gttcggccgc cggacgctgc cgcagatgga
    19621 tctggcgtcg gtgtcgtgcg ccatccaaaa cctgtggctg gcggcgaggt ccgagggcct
    19681 gggtatgggg tgggtttccc tgttcgatcc gcaacggttg gcggcgctgc tggcgatgcc
    19741 cgccgacgcc gagccggtgg ccatcctgtg cctcgggccg gtgcccgaat tcccggatcg
    19801 gccggcgttg gagctggacg gctgggccta cccgcgaccg ctgtcggagt tcgtctccga
    19861 gaaccaatgg ggtcagccgt cgccggcggg gcgcgtgcac ccgtaagtgg caccaggctg
    19921 cggtatcgtc gccggtcgag gtgacatgac ggctacattt gctcaagacg cgcaggatgc
    19981 gcagggcgcc caggacagca aagacagcgt gctgatggtg cactggcatg accttgggcg
    20041 atatctgggc gcctacggcc acgccgacgt atccagcccg cggctggacc gacttgcggc
    20101 cgagggcatc ctgttcacca gggcccatgc cacggcgccg ctgtgctcac cgtcgcgcgg
    20161 atcactgttc accggccgtt acccgcagag caacgggctg gtcggcctga cccaccacgg
    20221 ctgggaatac cgcagcggcg tccgcaccct gcctcagatg ctgtccgaat caggttggta
    20281 ttcagcactt ttcggtatgc aacacgagac ttcatatccg agacggctgg gtttcgacga
    20341 attcgacgtg tcgaactcct actgcgagta cgtggtggac aagaccgcac agtggttacg
    20401 ggaggccccc gagcagcttg cgggccaacc gttcctgttg accgcgggat tcttcgagac
    20461 ccaccggccc tacccgcaag accgctacca gccggccgac agcgccggtg tcgatctccc
    20521 cgactacctg cccgatacgc ccgagattcg cggcgacctc gccggcttct acggggcgat
    20581 cgccgcggcc gacgccgcgg tgggccgact gctggacacg ctggccgaaa cggggctgga
    20641 cgccagcacc tgggtggtgt tcttcaccga ccacggcgcc gcgttcccgc gcgccaaatc
    20701 cacgctgtac gacgccggaa ccggcatcgg catgatcgtg cgcccaccga ccaaccgggc
    20761 catggccccc cgggtgtacg acgagctgtt cagcggcgtc gacttggtgc cgacgctgct
    20821 cgggctgttg ggtcgcgacg tgccggccga cgtcgagggc gtgtcgcacg cggccgccct
    20881 gctcgcgccg gacccgcagg ccgaaccggt ccgcaaccac gtgtacacga tgaagaccta
    20941 ccacgactcg ttcgacccga ttcgcgctgt tcgcaccaag gaatacagct acatcgaaaa
    21001 ctacgcgtcc cggccgctgc tggatctgcc ctgggacatc aaggaaagcc catccggggt
    21061 ggccgtcgcc ccgttggtcg gtggtccccg cccgaagcgg gaactctacg atctacgcgc
    21121 cgatcccacc gagaccgaca acctgctggc gcgtcccgac gcgggcgtgg atgcggtcgc
    21181 cgccgatttg gctgtccgcc tgcatgattg gcgccagcgt ctcgacgacg tcatcccatc
    21241 ggagttcgcc ggtgcccgca ttgcggggcg ctacaccgaa acgtatctgc gcattcatca
    21301 caccaagccg accagccggt cggcgatcgc cgtcgaccgc ggcatcgagg agtaagccgc
    21361 cgggcgcggc ctacccgccc gagcgaggcg cgccaccaac ctgggcgacg atgaacacgc
    21421 gccggaacgg gaagatcgtc gtgccgtcgg cccggggcgg gtacgcgtcg tccagcagcg
    21481 ggatgagctc ttggcggaac tgctcccagc cctcgtcgtc gagccgctcg cgcaccggga
    21541 ccagcgccgt gccggtgatc cactccagca ccggatgctc accggtcagc tgatgcagat
    21601 aggtggtctc ccagacgtcg acctggcatc cggcgtccag cagctcattg gcgtactgga
    21661 tcggcgggtg aaccaccgct ccaacgcgaa acggtatgtc gcgcatgatt tttgcgtagg
    21721 gctcacggcg ggccagcgcc cgcaccgcag cgtgcgaggg cgtttcgaag ttgcccggga
    21781 tctggatgcc gatccacgat ccgggcgcca actcgccggc ccaccggatc agcaggtcgg
    21841 cgtgctcggg cacccaatgc agggcggcgt tgctgaccac cacgtcggtg tcgggcttcg
    21901 gtttccagtc ccgcaagtca gcggtgacgg cgttgatgcc gcgttccttg gcggcggcga
    21961 ccatctccgg cgagctgtcg atcgcctcga tcacggcctc gggccagcgc cgcgccaggt
    22021 aggccgtgag gtggcccggc ccacagccga ggtcgaccac ccggcgcgcc cgctcggcgc
    22081 cgacgcgcga cagcaaatcg tagaaggggc ggctgcgatg gtccgcaaag gccaggtaga
    22141 tgtcgggatc ccacatgtcg gtcctcctga tcaacaccac cgcgggtcgg ctgatggaca
    22201 atccgtatta ccgtatgccc tcccagtagc caccgagtgg ggtttaccat cgcctgcgtg
    22261 acgcccgatg cggatccagt gggccggcga gttcgtgttc cggatgttcc cggcgccgac
    22321 gtccccgccg gtgccgaggg attgccgtcg cgctcggcgt tgtcgacccg ccagcgaatg
    22381 gtcgtcgctg cgaccgcgat gtccgatttc gcgctgcgca ccgcgatcgc atcggtgctc
    22441 tcggccgcgg tgacaccggc cgtgatcgcc agctccacgc gccacggcgg tgagcgcgac
    22501 aacctgcgtt tctacgccga cctggggtcc gagcgggatc cggcgaagtc gttcccggcg
    22561 cccaccgagt tgccgcgggt gtcgtcgcgg ccggccaacc ggcttgcggc gcgaatcgcg
    22621 ggcggcaccg tggagaacat ctcgtttgcc agcagcttcc gggccgtcaa tcccgcgatg
    22681 cgcacacgat gggacgcgtg gaaatccaat aacgtcgtgc acgcccagca ttggcgccac
    22741 ggcgacggac cgcgtcccac gctgtgcgtc atccacggct tcatgggggc gtcatatctg
    22801 gccaacgggc ggttcttcac cctgccgtgg tattaccgct ccggctacga cgtcctgatg
    22861 tacacgttgc cgttccacgg cgcacgggcc gaaaagttct cgccgttcag cggtttcggc
    22921 tacttcgccg gcggcctcag cggtttcgcg gaggcgatgg cccaggccgt gcacgacttc
    22981 cggtccatca tcgactattt gcgccacacc ggcgtgcagc gcatcgcgtt gaccgggata
    23041 tcgctgggcg ggtacacctc cgcgctggtg gcttcggtcg acgatcggct cgacgccgtc
    23101 atccccaact gcccggtggt gactccggcg acgatgttcg aacaatggtt cccggccaac
    23161 aaactcgtgc gactgggact gcggcagtcc cacatcagcc gtgacgagct ggccgccggg
    23221 ctgtcctacc actgcccgct gacctaccgg ccgctggtgc cgacgaaccg ccggatgatc
    23281 atcaccggcc tcggcgaccg gatggcgacg cccggtcagg ccgtgctgct ctgggaacat
    23341 tgggatcgct gtgcgctgca ctggtttccc ggcagtcacg tgctgcatgt cagtcagctg
    23401 gactacctgc gccggatgac gccgttcctg cagcagttca tgttctagcc ggttgcggtg
    23461 cccggtgggc gccggccacc ggagtgggcc agaccagccg cgccagcagg ccggcggacg
    23521 cgtcggtgtg cccgtccagc cgttgcgcgg acaacagatc caatgccgtc agcgccgcca
    23581 ggtgattccc gcgttgcggc gtcagtccgg ccagcggcgc agcgccgtcg ggcggcattt
    23641 cggtgaggaa cgcgcacgcc gccgcgatgg tgtcgtgggt tgcgtcaccg gcgatctcca
    23701 gcgccatgca gacctcgcgc gccgggtacg accggatcgc ggccaaggtt tcgggcaacc
    23761 ggtcatcgat gttgacctgg tatgccgcaa tgtaatccga gggcccccgc cgcaccccgc
    23821 gccaggattc gcgggcgccg ggcgccagca gccgtggcac gtcgtcgggt gccaccggag
    23881 tggcttccca cccgatctca cggaggtggt cggccagccg gcgcgccgcg acctgggcgg
    23941 tctcgtgcaa cgggattcgc gatgagcgcg cctgcaacgc cggcagattg tcggccgccg
    24001 acaccgtgag cccgatccag gtctcccgcc tcgacgcatc ggatgcgttg tcgcggctgg
    24061 tgattcggat cttgtcggcg cggatcccgt agcggtccag gtaccgggcg atcagcccga
    24121 gcggcagcac gtccgagtca ccgacgggtg gccccacctg caacagcacg gtcgtcgtcg
    24181 tcgcggattc ggttgtggca ccgtcggttt ggtcgggccg gcggcccatc atcgcgaagc
    24241 gtcgccgcag gatggtggtg aagtgcaagc cccgccacca tccgaacagc aggatcaccg
    24301 cggccgcggc gatgccgagc acccaatggt cgcgagtcga ccgccacggg tatgccatcg
    24361 ccgcgggcac cacggccagc agcgccagcg tgatgcggcc gctgcccggt gtcggtatcg
    24421 agcgatgcag gctcacgggg tggtctcctt ctttcgggtg atcgcgacaa tcgcgccggc
    24481 gaccgcgaca agcaaagcca gagcggcggt tccggcgaac gcgacggtcc gcggagtggt
    24541 gtccttgtgc gccggcggcg ggggcgccgc gaccggcttg gtgctcgcca taccgggctg
    24601 gtcgccggcc ggcagttccc aggtcaacgc ggcgaccggg tccacgctgc cggcgccgac
    24661 gacgtcggac ggggcgcggg cgccgttgtg cgcggtcgcg gtgatgcgcc gcatcacctg
    24721 cgtggcggtc agttgcggat acttgctgcg aaccagcgcc gcgacgcccg acacgtatcc
    24781 ggctgcatag ctggtgccgc tcagcgggat catctgctgg tggtcgtcgg gcaagccatt
    24841 agcgagaccg ccgccgtcgc gattgctcac cgacacaatg tcttcgcccg ggccggcgat
    24901 acccacccac ggtccggcca tggagaactt cgacggctgc ccgtccggcg tcaacgccgc
    24961 cgtcgacagc acgtacggct gccaccacga tgggatggac accgacttga cgccggccca
    25021 gttgcgcgga tcgtccggat ggctcaggtc ggtgagtgga ttggactcac atgacgtgcc
    25081 gccggcgatc gatccggtct gcccgctgtt gcccgcggcc gtcacgatca ccgcatcctt
    25141 gtccacggcc gcgtagcgga tcgcggcgcc gagcgcggcc tggtcgatcg tacggtcggc
    25201 gggcaggcag gttatcgccg agatgttgat caccctggcg ccgaggtcgg ccgcgtgcac
    25261 gatcgcccgc cccagcgtcg cgacgtccag ggacgcctgc gccagcagcg gatcgccgcc
    25321 cggcgtacgc ggcgagaact tcgcggaagc cgtcctgatc gacagcacgc gcgccgcggg
    25381 cgccacgccg gagaaaccgt cgtcaccggg ctggccggcg atgattccgg cgaccagggt
    25441 gccgtggccg tcgcagtccg tcaggccgtc ggtcgtctcg acgaaatcgc ccccgggatc
    25501 gacgttgggc agccgcgggc caggccgtac cccggtgtcg atgaccgcca ccagctggcc
    25561 ctcgccgcgg gaaaactgcc acgccgctgg gagattcaac atcgcctgac ccggcgtcag
    25621 cacacccggg tcgctgcccg ggatgacccc ggaggcgctg cacggcccgc gctgctccat
    25681 cggctgaccg ggccccgggg tgccgctggg tggcggcact cccggatcga ccgccggcgg
    25741 gctgatcgcc agcgccggcg gacaagccca catcaccccg gcctgcagga acaccagggc
    25801 cgctgtgacg caggctgatc cggaccggat catcgattga gcacccagct gaacagacca
    25861 accaggtagg ccatgactgg gatcagcgat gcatccagcc ccgccgcaac gaagcccagc
    25921 agccggcgca acggcagcga atagctctcc ggcgaggcga tgcgcgggtt cagtgccacc
    25981 accacccaca ccgacagcag cgcgacgagc accagcaccg cacccagggc acccgggtaa
    26041 cgacccgtcg cggcgtagag caccaggagg acacccgcga ccagataggg ctgagcgagc
    26101 agccacgcct tgcaggcggc cgaatcccac accctggcgc gcaggaccga cgcggccgcc
    26161 gttgccgcaa ccacgtacca gccccatccg ctcagcgact cggggcgcag cgcgaccgcc
    26221 accgacccca gaacgctgag cagcaccgcg gcggcgatga agccgctctg gtgggcgtcg
    26281 gcggtccgca cccgccgcgg cagatcctcg agcacccgca gcgacggcgc cgacgggctc
    26341 gggtcccccg gcgccgggat gaccggcagt gggaagcggg cccatagcgc agacagctgg
    26401 ggcgcctgga ttgtgaccag cagcgccgcc acgatcaggc cgcagccgat gctcagt
//
LOCUS       DASSGQ010000241        17523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1384228,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000241 DASSGQ010000000
VERSION     DASSGQ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 17523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17523
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384228"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="VFI55_10630"
     CDS             <1..1302
                     /locus_tag="VFI55_10630"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HET7074521.1"
                     /translation="GDPLAVGDQYLTGDLTAGQIESLVSADAAALAWPMRAIYRIWPD
                     FEAHPQIDASSATIKARAAQRAFESFGDGIVWAVVDSGIQADHAHFAGYATLTDASVA
                     DLHRDFTVDSDDPTLALVDNDGHGTHVAGIIAGGLQNWQPATGNRKVVAGEKRFNAAS
                     PDGAFPIIQPRAIADIDRLAGMAPHAKLVSLKVLGDGDAATRVSRVMRALAYVRERNA
                     ATEKMPRIHGVNLSLGYAFAAEWFACGQSPLCVEVDKLVQSGVVVVAAAGNTGYVTLN
                     PKFADVTKFSAGVTINDPGNAERAITVGSTHRDSPHTFGVSYFSSRGPTGDGRAKPDL
                     VAPGERITSAAAGKNLRAVQDAHLDDLTGAAVYIEDSGTSMAAPHVSGAVAAFLSVQR
                     EMIGKPEDIKKIFVASAISLGREQAFQGAGMVDLMRALQSV"
     gene            1343..2698
                     /locus_tag="VFI55_10635"
     CDS             1343..2698
                     /locus_tag="VFI55_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-threonine protein kinase"
                     /protein_id="HET7074522.1"
                     /translation="MTDTIANRPTWRLTFDKDGGVDPQTLTELKQGIADNRLTDLVLF
                     SHGWNTDEAAANSLYDRWFQLLADQVDANVKVGFVGVRWPSQLWRDEPIPDFDPAPKR
                     DAGGAAALEERPTTAAGPPTIDPAQLEDLKEIFPAGTAQLDAIAALLTQEPSQEQVNA
                     LFAAMREFSKATLTGFNDGEAKQSDQPGMLGAQQNPNDVFTKFADKLAEAGVDFSDGG
                     GTAGLGDFANKLLNGAKEALRQLTYWQMKNRAGVVGENGLGPVIGQLAGQFDGLRIHL
                     IGHSFGARLVSFALAGLPAANPSPVKAVTLLEGAYSRFAFSNPLPFLTELDEPRAGAL
                     AGRLDRIDGPLTVCFSSHDLALGIFYPLASAAAGDDSAALSDPLSRWRAMGSDGAFGP
                     DPQPLGRAGSVYPFKVGEILNLDASDVVTRGGPPAGAHSDIFYPQLAWVVAAAGGLNP
                     K"
     gene            complement(2724..3167)
                     /locus_tag="VFI55_10640"
     CDS             complement(2724..3167)
                     /locus_tag="VFI55_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009955995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET7074523.1"
                     /translation="MSEPRPPRYLKPMNRVMMAVQRLGIPTGPAMVLTVLGRKSGRPR
                     ATPMTPFEFRGGLYAVAGYPGADWAANARAAGTGTLSRGRRSRQVRIVELTAEQARPV
                     LRAFPAEVPVGVAFAKRSGMVRDGTADEFEALAGRIAVFRFEPVA"
     gene            complement(3169..4020)
                     /locus_tag="VFI55_10645"
     CDS             complement(3169..4020)
                     /locus_tag="VFI55_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET7074524.1"
                     /translation="MTIVVTGATGNVGRPLVAELVAAGARVRAITRRPGTAGFPSGVE
                     AAGSAAEALTGASAVFLNSRALGEQLPTVVAECRRAGVAKLVALSAINADDDFSKQPS
                     RSRGDRNKEVEQLAVESGLAWVSLRPTVFATNFAGMWSAQIRAGDVVAGPYAAASSAP
                     IVESDIAAVAARALLTDELVGERIPLTGPQAFTNSELVEVIGAVLGRPLQYREVPSDL
                     VRQRFIGLGFSADFADAYMAMLAETLDKPALVTHDVEKILGRPATGFAQWISAHRDLF
                     TESAQGA"
     gene            4131..5042
                     /gene="sigI"
                     /locus_tag="VFI55_10650"
     CDS             4131..5042
                     /gene="sigI"
                     /locus_tag="VFI55_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigI"
                     /protein_id="HET7074525.1"
                     /translation="MNQQSPSDQITEAWRRHRPYLVNLAYQMLGDVGDAEDVAQEAFL
                     RLSRALENDVNEIDDVRGWLTVVASRLCLDQVRSARARYERPGGPDPLEQPVSRRPDP
                     GDQVTLDDEVRTALLEVLRRLSPGERVAFVLHDVFAVPFDTIAETVGRPVGTCRQLAR
                     RARAKFSTAQPKFNDVASAEHQLVTEKFITACANGDIAGLTAVLDPAVWGVGTVLADP
                     APPPQVNHGPDAVATNLLRYLGPEVTLVSGPAGLGAPPARGGPVVLAFADRRLFAAVV
                     LTIRGRLVTKIEAIADPSARAGAIQNT"
     gene            complement(5039..6268)
                     /locus_tag="VFI55_10655"
     CDS             complement(5039..6268)
                     /locus_tag="VFI55_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HET7074526.1"
                     /translation="MNERDEFLRDRLRPDQPSSPAARPPGPHPGPRPAPPAPPPVPPR
                     VAQRPAPAHPTPPPRWPGPPPRSGPRPAPPPPPSSPPSPSSPPSPVPWTEPAFAPAPP
                     RVPEPPDRGWRRILRRATFGLIQLGPSPAQRQEAQFEAAIRTPLHDNYKVGVLGKGGV
                     GKTSVAASVGSLFAELRRQDHVVAIDADTAFGRLSCRIDPRSAGSFWELTSDKNLQSF
                     TDVVGRLGRNPAGLHVLGGEPASGPRRVLDPAIYREAALRLDRHFTISVIDCGSTMDS
                     PVTQEVLRDLDALIVVSSPWADGASAAARTMEWLSDQGQTALLRNTIVVLNDSDGHAD
                     KRTRELLAREFVDHGQPVVEVPFDPHLRPGGVIDVDGEMAPATRRRFLQITATIAGYF
                     AARSDRGRNRPAGGDGG"
     gene            6924..11519
                     /gene="gltB"
                     /locus_tag="VFI55_10660"
     CDS             6924..11519
                     /gene="gltB"
                     /locus_tag="VFI55_10660"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HET7074527.1"
                     /translation="MTPKRVGLYNPAFEHDSCGVAMVVDMHGRRSRDIVDKAITALLN
                     LEHRGAQGAEPRSGDGAGILIQVPDAFLREVVDFELPPPGEYATGIAFLPQSSRDAAA
                     ACAAVEKIAEAEGLHVLGWRNVPTDDSSLGALSRDAMPTFRQVFMAGASGMTLERRAY
                     VIRKRAEHELGTKGPGQDGPGRETVYFPSLSGQTFVYKGMLTTPQLKAFYLDLQDDRL
                     TSALGIVHSRFSTNTFPSWPLAHPFRRIAHNGEINTVTGNENWMRAREALIKTDIFGS
                     EDDVEKLFPICTPGASDTARFDEVLELLHLGGRTLAHAVLMMIPEAWERHESMDPARR
                     AFYQYHSSLMEPWDGPASMTFTDGTIVGAVLDRNGLRPSRIWVTDDGLVVMASEAGVL
                     DLDPGKVVRRMRLQPGRMFLVDTAQGRIVADEEIKAELAAEHPYQEWLDKGLVPLDDL
                     PAGKYVRMPHDRLVKRQLTFGYTYEELNLLVAPMVRAGAEPIGSMGTDTPVAVLSQRP
                     RLLYDYFHQLFAQVTNPPLDAIREEVVTSLQGVTGGEQDLLNPDERSCRQIVMQQPIL
                     RNYELAKLVNLDPDEMVNGRRHDMRSKVIRCLYPVAEGGAGLAAALTEVREQASAAIT
                     EGARIIILSDRASDERMAPIPSLLAVAAVHHHLVRDRTRTHAGLVVESGDAREVHHMA
                     MLIGCGAAAVNPYLAFESIEDMLDRGLFDGAGNGIDRKKALNNYVKAAGKGVLKVMSK
                     MGISTLASYTGAQLFQAVGVSQEVLDEFFTGLSCPTGGITLEDIAADAAARHAVAYLD
                     RPDERAHRELEVGGEYQWRREGEYHLFNPETVFKLQHATRTGQFQIFREYTRLVDDQS
                     ERMASLRGLLKFRADVRPAVPLDEVEPASEIVKRFSTGAMSYGSISAEAHETLAIAMN
                     RLGGRSNSGEGGEDVKRFERDPNGDWRRSAVKQVASARFGVTSHYLTNCSDIQIKMAQ
                     GAKPGEGGQLPGHKVYPWVAEVRHSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNANP
                     SARVHVKLVSENGVGTVAAGVSKAHADVVLISGHDGGTGATPLTSQKHAGAPWELGLA
                     ETQQTLLLNGLRDRIVVQVDGQLKTGRDVIIGALLGAEEFGFATAPLVVVGCIMMRVC
                     HLDTCPVGVATQNPVLRERFTGKPEFVENFFLFIAEEVREYMAELGFRTFNEMVGQVG
                     SLDTTLARAHWKAHKLDLTPVLHEPESAFMNQDLYCSSRQDHGLDKALDQQLIVMSRE
                     ALDSGSPVRFSTAISNVNRTVGTMLGHELTKAYGGQGLPDGTIDITFEGSAGNSFGAF
                     VPRGITLRVYGDANDYVGKGLSGGRIVVRPSDNAPQDYVAEDNIIGGNVILFGATSGE
                     VYLRGVVGERFAVRNSGAHAVVEGVGDHGCEYMTGGKVVILGRTGRNFAAGMSGGVAY
                     VYDPHEELPENLNEEMVELETLDEDDADFLYGTIQAHVDATDSAVGQRILADWRGQQR
                     HFLKVMPRDYKKVLQAIAEAERDGVDIDKAIMAAAHG"
     gene            11512..12987
                     /locus_tag="VFI55_10665"
     CDS             11512..12987
                     /locus_tag="VFI55_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HET7074528.1"
                     /translation="MADPTGFLKFTHRELPQRRPVPLRLRDWNEVYEEFDDHTLRHQA
                     TRCMDCGIPFCHNGCPLGNLIPEWNDLVRRGRWRDAIERLHATNNFPDFTGRLCPAPC
                     EPACVLGINQDPVTIKQIELEIIDHAFDEGFVVPLPPDRLTGKTVAVVGSGPAGLAAA
                     QQLTRAGHSVTVFERDDRIGGLLRYGIPEFKMEKRILDRRLDQMRAEGTEFRPSVNVG
                     VDITAEQLCADFDAVVLAGGATAWRDLPIPGRELDGIHQAMEYLPWGNLQALDELDAA
                     DLDEDGEPPITAKGKKVVIIGGGDTGADCLGTVHRQGATEVHQFEIMPRPPDARAEST
                     PWPTYPLMYRVSAAHEEGGERVFSVNTEEFVGKDGHVTALKVHEVTMRDGRFVKVEGS
                     DFDLEADLVLLAMGFVGPEKPGLLTDLGVDFTERGNVARGADFDTSVPGVFVAGDMGR
                     GQSLIVWAIAEGRAAAAGADRYLMGSTALPAPIKPTAAPLQ"
     gene            complement(13374..14225)
                     /locus_tag="VFI55_10670"
     CDS             complement(13374..14225)
                     /locus_tag="VFI55_10670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET7074529.1"
                     /translation="MRFAFKTSPQNTTWAQMLDVWRAADDIDVFDSGWTFDHLYPIFT
                     DSSGPCLEGWTTLTALAQATTRLRLGTLVTGIHYRHPAVLANMAAAVDIISNGRLELG
                     IGAGWNEEESGAYGIELGTIKERFDRFEEACQVLVSLLSEETTDFDGKYYQLKGARNE
                     PKGPQRPHPPICIGGSGEKRTLPITARYAQHWNFAGGPPELFAQKRDVLAARCADIGR
                     DPKEITKSAHLRLEADRNYGKVLEDAAALAAEGLDLGIVYIAPPHDPAVLEPLAEAIR
                     DSGLWSQ"
     gene            14339..15343
                     /locus_tag="VFI55_10675"
     CDS             14339..15343
                     /locus_tag="VFI55_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET7074530.1"
                     /translation="MDPVTALRQIAYYKDRSRQDPKRVMAYRNAADIVEGLSDAERER
                     HGQANSWQTLVGIGPKTAKVIDQAWSGREPDALVELRDAAEDLGGGDVRAALRGDLHL
                     HSNWSDGSAPIEEMMATAAALGHEYCALTDHSPRLTVANGLSPERLRRQLDVIDGLRD
                     RFAPMRILTGIEVDILDDGSLDQEPELLERLDVVVASVHSKLSMDSAAMTRRMVRAVS
                     DGHADVLGHCTGRLVAGNRGIRSESKFDAEKVFTACRDHGTAVEINSRPERRDPPTRL
                     LKLALEIGCVFSIDTDAHAPGQLDFLGYGAQRALDAGVPVDRIVNTWPAEKLLTWTAS
                     "
     gene            15731..16831
                     /locus_tag="VFI55_10680"
     CDS             15731..16831
                     /locus_tag="VFI55_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET7074531.1"
                     /translation="MTTRGKYTDVPAPYSWEVPSAGETRFTWEYDEGRARLLSLYQKG
                     KDKQWDAQSRIDWSQDVDPMNPVGLPDEFHPLFGSPMWDAADESRRAEMRQHSQAWQF
                     SQFLHGEQGAMVCAAKIVEVVPDLDAKFYAATQTMDEARHVEAFSRFLQEKVDLVYPI
                     NKNLTALLEDTLRDSRWDMPYLGMQVLIEGLALAAFGTLRDLAPQDSLAKQLLAYVMQ
                     DEARHVAFGRISLKDYYSALTEAERDEREEFVVDACYLMRDRFRGEEVFETLGMNVQE
                     CAEWVDTSPVMIQFRSHLFSRIVPIVKDIGLWGDKVQKAFRDMGVHDMGGFDIEALMK
                     ADEDQADALDKTHADMADRALEVDQVIAAGAS"
     gene            complement(16947..17034)
                     /locus_tag="VFI55_10685"
                     /pseudo
     CDS             complement(16947..17034)
                     /locus_tag="VFI55_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus biosynthesis protein TadE"
     gene            complement(17070..17198)
                     /locus_tag="VFI55_10690"
                     /pseudo
     CDS             complement(17070..17198)
                     /locus_tag="VFI55_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
BASE COUNT         3058 a         5792 c         5874 g         2799 t
ORIGIN      
        1 ggcgacccgc tcgcggtcgg cgaccagtac ctgaccggcg acttgaccgc cggtcagatc
       61 gaaagcctgg tgtcagccga tgccgcggcc ctggcatggc cgatgcgggc catctaccgg
      121 atctggccgg atttcgaggc tcacccgcag atcgacgcgt cgtcggcgac gatcaaagcg
      181 cgcgcggccc agcgcgcctt cgagagcttc ggcgacggga tcgtctgggc ggtggtcgat
      241 tccggcatcc aggcggacca cgcgcacttc gccgggtatg cgacgctgac cgacgcgtcg
      301 gtggccgatc tgcaccggga cttcaccgtc gacagtgacg acccgaccct tgccctggtc
      361 gacaacgacg ggcacggtac gcatgtcgca gggatcatcg ccggcggcct gcagaattgg
      421 cagcccgcca ccgggaaccg caaggtggtc gcgggggaga agcgcttcaa cgccgcctcg
      481 cccgacggcg cgttcccgat catccaaccc cgcgccatcg ccgacatcga ccggctcgcc
      541 ggcatggcac cacacgccaa gctggtcagc ctcaaagtgc tcggcgacgg cgatgcggcc
      601 acccgggtga gccgggtcat gcgggcgttg gcctacgttc gcgagcgcaa cgccgccact
      661 gagaagatgc cgcgcattca cggcgtgaac ctcagcctgg gatacgcgtt cgccgccgaa
      721 tggttcgcct gcgggcagag cccattgtgc gtggaagtcg acaagctggt gcaatccggc
      781 gtggtggtcg tggcggccgc tggcaacacc ggatatgtca cgctcaaccc gaaattcgcc
      841 gatgtcacca agttcagcgc gggcgtcacc atcaacgacc ccggcaacgc cgaacgcgcg
      901 atcaccgtcg gctccacgca ccgcgattct ccccacacct ttggcgtgtc gtacttctcc
      961 tcgcgcggcc cgaccggaga cggccgggcc aagccggatc tcgtcgcgcc cggcgaacgg
     1021 atcacgtcag ccgccgccgg taagaacttg cgcgccgtgc aggacgccca tctcgacgac
     1081 ctcaccggcg ccgccgtcta catagaggac agcggcacca gcatggccgc accgcacgtc
     1141 tcgggtgccg tcgcggcgtt cctgtccgtg cagcgcgaaa tgatcggcaa acccgaagac
     1201 atcaagaaga tcttcgtggc gtcggccatc tcgttgggcc gcgaacaggc cttccagggt
     1261 gccggcatgg tcgatctcat gcgcgcactg caatcggtct gagaacaccg caacaccaca
     1321 cgacaagaca ggacacctcg agatgaccga caccatcgcc aatcgcccca cctggcgtct
     1381 tacgttcgac aaggatggcg gcgtcgaccc acagacgttg accgagctga agcagggcat
     1441 cgccgacaac cggctcaccg atctggtgct gttctcccac ggctggaaca ccgacgaggc
     1501 ggcagcaaat tcgttgtacg acagatggtt tcagctcctc gccgaccagg tcgacgcgaa
     1561 cgtaaaagtg ggattcgtcg gcgtccgctg gccttcccag ctctggcgtg acgagccgat
     1621 acccgacttc gaccctgccc cgaagcgcga cgcgggcggc gccgccgcgc tggaggagcg
     1681 gccgaccacc gcagccgggc cgcctaccat tgaccctgcc caactggagg acctgaaaga
     1741 gatcttcccg gccggcaccg cccagctgga cgcgatcgcc gcactgctga cccaggaacc
     1801 atcgcaggaa caggtgaacg cactattcgc cgcgatgcgc gagttcagca aggccaccct
     1861 caccggcttc aacgacggcg aagccaagca gtcggatcag cccggaatgc ttggcgcgca
     1921 gcagaacccc aacgacgtct tcacgaagtt cgctgacaag ctcgccgagg ccggtgtgga
     1981 cttcagcgat ggcggcggca ctgccggcct cggtgacttc gcgaacaagt tgctcaacgg
     2041 cgccaaggag gcgctgcgcc agctcaccta ctggcaaatg aagaaccggg ccggagtggt
     2101 cggcgagaac ggactcgggc cggtcatcgg ccagctcgcc ggccagttcg acggcttgcg
     2161 aatccatctg atcgggcaca gcttcggcgc gcgcctcgtc tccttcgcgc tggccgggct
     2221 ccccgcggcc aatccgtcac cggtgaaggc ggtcacgcta ctggagggcg cctactcacg
     2281 ctttgccttc agcaaccccc tgccgttcct caccgaactg gacgaaccgc gcgctggtgc
     2341 actggccggc cggctggacc gcatcgacgg gccgctaacc gtctgcttct ccagccatga
     2401 cctcgccctg ggcatctttt acccgctggc gtccgcggca gccggtgacg actcggccgc
     2461 attgtccgat ccgctctccc gctggcgcgc gatgggatcc gacggcgcct tcgggcccga
     2521 cccgcagccg ctgggaaggg ccggctcggt ctacccgttc aaggtcggcg agatcctgaa
     2581 cttggacgcc tccgacgtcg tcacgcgcgg cggtccgccc gccggcgccc acagcgatat
     2641 cttttacccg cagctggcct gggtcgtcgc cgccgctggc ggcttgaacc cgaagtaggc
     2701 gcagagcgat tgagcctcat ggctcacgcg accggctcga atcggaagac cgcgatccgg
     2761 cccgccaggg cctcgaattc gtcggccgtg ccgtcgcgga ccatcccgga gcgcttggcg
     2821 aacgccacac cgaccggcac ttcggcggga aacgcccgca gcaccgggcg tgcttgctcg
     2881 gcggtcagtt cgacgatcct gacctgccgc gatcgtcggc cccggctcag cgtgccggtg
     2941 cccgcggcgc gggcgttggc cgcccagtcg gcacccggat agccggccac cgcgtagagg
     3001 ccaccccgaa attcgaacgg cgtcatcggg gtggcgcgcg gacggcccga cttgcggccg
     3061 agcaccgtca gtaccatcgc gggaccggtc ggtattccca gccgctggac ggccatcatc
     3121 accctgttca tgggtttgag gtagcggggt ggacgtggtt cggacatgtc aggctccttg
     3181 tgctgattcg gtgaatagat cgcggtgcgc ggaaatccac tgagcgaacc cggtagcggg
     3241 ccggccgagg atcttctcca cgtcgtgggt gaccagggcg ggcttgtcca gcgtctcggc
     3301 gagcatcgcc atgtaggcgt cggcgaagtc ggcgctgaag cccaaaccga tgaaccgttg
     3361 ccgcaccaag tcgctcggga cctcccggta ctgcagcggg cgacccagca ccgcaccgat
     3421 gacctcgacc agctcggagt tggtgaacgc ctggggaccg gtcaggggga tgcgctcacc
     3481 gaccagctca tcggtgagca gggcgcgcgc cgcgaccgcc gcgatgtcgc tttcgacgat
     3541 cggcgcggac gacgccgcgg cgtagggccc ggccaccaca tcgccggcgc ggatctgcgc
     3601 cgaccacatg cccgcgaagt tcgtcgcgaa caccgtgggc cgcaggctca cccaggccag
     3661 tccggattcg acggcgagct gttcgacctc tttgttgcgg tctccgcgag agcgcgacgg
     3721 ctgcttggag aagtcgtcgt cggcgttgat cgccgacagc gcaaccagct tggcgactcc
     3781 ggcgcgccgg cactcggcga ccactgtggg cagctgctcg cccagcgcgc gggaattgag
     3841 gaacaccgcc gacgcgcccg tcagggcttc ggccgccgag ccggccgcct cgacgccgga
     3901 cggaaagccg gcggtgccgg gtctgcgggt gatcgcgcgc acgcgggcgc ccgcggcgac
     3961 gagttcggca acgagcgggc gtccgacgtt gccggtcgct ccggttacga cgatcgtcat
     4021 gggaggttcc ttccctggaa ttttctgtgg tgctgctacg acggcgcgca gggtgggaaa
     4081 gttacaagcg gaacgcgtaa ctttttgccg catcagatcg tcgaagactc atgaaccagc
     4141 agtcacccag cgaccagatc accgaggcct ggcgccggca ccgcccgtat ctggtcaacc
     4201 tcgcctatca gatgctgggc gacgtaggtg acgcggaaga cgttgcgcaa gaagcgtttc
     4261 tacggctctc acgtgctctg gagaatgacg tcaacgagat tgacgacgtg cgcggctggt
     4321 tgacggtggt ggcaagccgc ctctgcctcg accaggtgcg ttcggcccgt gcccggtacg
     4381 aacgtccggg cgggcccgat ccgctggagc agccggtgtc gcgccgcccg gacccgggtg
     4441 accaggtcac cctcgacgac gaggtccgca ccgcattgct ggaagtcctg cgccggctca
     4501 gtcccggcga acgcgtcgcg ttcgtgctac acgatgtgtt cgcggtcccg ttcgacacga
     4561 tcgccgaaac cgtgggccgg cccgtcggca cctgccgcca gctggcgcgc cgtgcgcggg
     4621 ccaaattcag cacggcgcag cccaagttca acgacgtcgc ctcggctgag catcagctcg
     4681 tcaccgagaa gttcatcacc gcatgcgcca acggcgacat cgccggattg accgccgtcc
     4741 tggatccggc ggtctggggc gtggggacgg tcctcgccga cccggcaccg ccgccacagg
     4801 tcaaccacgg accggacgcg gtcgccacca acctgctgcg ctacctcggg ccggaagtga
     4861 cgctggtcag cggtcccgcc gggctggggg cacctcccgc tcgcggggga ccggtagtgc
     4921 tggccttcgc cgaccgccgg cttttcgccg ccgtcgtgct gacaatccgc ggtcgcctcg
     4981 tgaccaagat cgaggccatc gcagaccctt cggcgcgggc cggggccatc cagaacacct
     5041 agcccccgtc gccacccgcc ggacggttac gcccacggtc tgaccgggcc gcgaaatacc
     5101 cggcgatcgt cgccgtgatc tgcaggaatc tgcgccgagt cgccggcgcc atttcgccgt
     5161 cgacatcgat gacacccccg ggccgcagat ggggatcgaa gggcacctcg accaccggct
     5221 gcccgtggtc gacgaactcg cgtgccagca actcgcgggt gcgtttgtcg gcgtggccgt
     5281 cggaatcgtt gagcaccacg atggtgttgc gcagcagggc ggtctggcct tggtccgaca
     5341 gccactccat ggtcctggcg gcagccgagg cgccatccgc ccagggtgag gacaccacga
     5401 tcagcgcatc cagatcgcgc agcacttcct gggtgaccgg tgagtccatc gtcgagccgc
     5461 agtcgatgac cgagatcgtg aaatggcggt ccagccgcag cgcggcctcc cggtagatcg
     5521 cgggatcgag cacccggcgc gggccggacg ccggttcgcc gccgagcacg tgcaatccgg
     5581 cgggattgcg gcccaggcgc ccaaccacgt cggtgaagga ctgcagattt ttgtcggacg
     5641 tcagttccca gaacgaaccg gccgaccgcg ggtcgatccg gcagctcaac cggccgaagg
     5701 cggtgtcggc atcgatcgcc accacgtggt cttgccgacg cagttcggcg aacagcgagc
     5761 cgacgctggc ggccaccgac gttttcccca cgccgccctt gcccagcacg ccgaccttgt
     5821 aattgtcatg aagaggggtc cggatggcgg cttcgaactg tgcctcctgc cgctgggcgg
     5881 gcgatggccc cagctggatg aggccgaagg tggcccgccg caggatgcgc cgccaaccgc
     5941 ggtcgggagg ttcggggacg cgcgggggtg ccggcgcgaa ggccggttcg gtccagggaa
     6001 ccggcgaagg cggggatgac ggcgaaggcg gggatgacgg cggcggaggg ggagccggcc
     6061 gggggccgga ccggggcggc gggccaggcc agcgcggtgg cggcgtcggg tgcgcgggtg
     6121 cggggcgttg agcaacccgg ggaggaaccg ggggaggagc cgggggcgca ggccggggac
     6181 ctgggtgagg accgggcggg cgggcggccg ggctgctggg ctgatcgggc cgcagacggt
     6241 cccgcaagaa ttcgtcgcgc tcgttcatgg gctcctcggg tgccgggtct tcgcgcatgt
     6301 ggccaaccgt gggcgctcct caagtgatga gcaccacgct ccaaccgtta acgcagtatt
     6361 cggaccagtc tgcatccagt gtccgtccag tatccggcac gagccgccgc gcggccttcc
     6421 cggtcgcgcg caaactttgc gggccgtacg tgggtgccac cgcgattcct tcggtcatcg
     6481 cgctgagcac gtctagtggg cgatcgcggc acggtggcgg ccccgcgact acgcgttcgc
     6541 agcaaactat cggccggggc tcagagggcg gctggtccgt cgggatcgat gccgcggcgc
     6601 gaaagccacc ccgcggtgaa accgcatgtc ggcgccggtg cgaaggtaga tcgcagcgaa
     6661 ttttcggtcc cggcggcggg cagttagact tgacgaagtt ggcatgtcaa gcgggttttg
     6721 tcatatcgtt ttacgacttg tcgagaccgg ttccaggcca ccgttcagcg ctgcacggac
     6781 gaaatctcta accggattat ctccgcctgg gggacagcct accgacaaga ccgacggggc
     6841 cttggcccgc aacctccggg tgcgggcccg acggagtcgg tcgtaacggc ctctggaacg
     6901 ttgtggtgaa gggtcaggtg catatgacgc ccaagcgcgt cgggttatat aaccccgcgt
     6961 tcgagcacga ttcgtgcggg gtagccatgg tcgtcgacat gcacggccgt cgcagccgcg
     7021 acattgtcga caaggcgatc accgccctgc tcaacctcga gcaccgcggt gcccagggcg
     7081 ccgagccgcg tagcggtgac ggcgcgggca tcctgattca ggtcccggac gcgttcctgc
     7141 gcgaggtggt ggatttcgag ctgcctcctc cgggcgagta cgccaccgga atcgcgtttc
     7201 tgccgcagtc gtccagggac gccgcggcgg cctgcgctgc ggtggagaag atcgccgaag
     7261 ccgaaggcct gcacgtgctg ggctggcgaa acgtacccac cgacgattca tcgctggggg
     7321 cgttgtctcg tgacgcgatg ccgaccttcc ggcaggtgtt catggcgggg gcctccggta
     7381 tgacgctgga gcgccgcgcc tacgtgattc gcaagcgtgc cgaacacgaa ctcggcacca
     7441 aaggtccggg ccaggacggg cccggccgcg aaacggtcta tttcccaagc ctgtcgggtc
     7501 agacattcgt ctacaagggc atgctgacca ccccgcagct gaaggcgttt taccttgacc
     7561 tgcaagacga tcggctgacc agcgcgctgg ggatcgtgca ctcccggttc tccaccaaca
     7621 ccttcccgtc ctggccgctg gcgcatccct tccggcgcat cgcccacaac ggtgagatca
     7681 acacggtcac cggcaacgaa aactggatgc gtgcgcgcga ggcgttgatc aagaccgaca
     7741 tcttcggttc cgaagacgac gtcgagaagc tgttcccgat ctgcacgccc ggagcttccg
     7801 acaccgcgcg gttcgacgag gttctcgaac tgctacacct gggcggccgc actctggctc
     7861 acgccgtgtt gatgatgatc cccgaggcct gggagcggca tgagtcgatg gaccccgcgc
     7921 ggcgggcgtt ctatcagtat cactcgtcgc tgatggagcc gtgggatggt ccggcgtcga
     7981 tgacgttcac cgacgggacc atcgtcggcg ccgtgctcga ccgaaatggc ctgcgcccct
     8041 cccggatctg ggtcaccgac gacggcttgg tggtgatggc gtccgaggcc ggcgtgctgg
     8101 acctggatcc gggcaaggtg gttcgccgga tgcggctgca gcccggccgg atgttcctgg
     8161 tggacaccgc gcagggccgc atcgtcgccg acgaggagat caaagccgag ctggccgccg
     8221 agcatcccta ccaggagtgg ctcgacaagg gtctggtgcc gctggacgat ctgccggcgg
     8281 gcaagtacgt ccggatgccc cacgatcggc ttgtcaagcg gcagttgacg ttcggttaca
     8341 cctacgagga actcaacttg ttggtggcgc cgatggtgcg cgccggcgcc gagccgatcg
     8401 gctcgatggg caccgacacc cccgtcgcgg tgctgtcgca gcgcccgcgc ctgctttacg
     8461 actacttcca ccagctcttc gcccaggtga ccaacccgcc gctggacgcc atccgcgagg
     8521 aggtggtgac cagcctgcag ggcgtcaccg gcggcgagca ggacttgctc aacccggacg
     8581 agcgctcctg tcgccagatc gtgatgcagc agccgattct gcggaactac gagctggcca
     8641 aactggtcaa cctcgacccc gacgagatgg tcaacgggcg ccgacacgac atgcggtcca
     8701 aggtgattcg ttgtctgtac ccggtcgccg agggcggcgc cgggctggcg gccgcgctga
     8761 ccgaggtccg cgagcaggcc tccgcggcga tcaccgaggg cgcgcgcatc atcatcctgt
     8821 ccgaccgcgc gtccgacgaa cggatggctc ccatcccgtc gctgctcgcg gtggcggcgg
     8881 tgcatcacca cctggtgcgc gaccggacgc gaacccacgc ggggctggtg gtcgaaagcg
     8941 gtgacgcccg cgaggtccac cacatggcga tgctcatcgg ttgcggggcc gcggcggtca
     9001 acccctactt ggcgttcgag tcgatcgagg acatgctcga ccgcgggctt ttcgacggcg
     9061 ccggcaacgg gatcgaccgc aagaaggcgc tgaacaacta cgtgaaggcc gccggcaagg
     9121 gcgtgctgaa ggtgatgtcc aagatgggca tctcgacgct ggcctcctac accggcgcgc
     9181 agctgttcca ggcggtgggc gtctcccagg aagtgctcga cgagttcttc accgggctgt
     9241 cgtgtcccac cggcgggatc accctggagg acatcgccgc cgatgccgcc gcccgccacg
     9301 cggtggccta cctcgaccgg cccgacgaac gcgcgcaccg cgaactcgag gtgggcggcg
     9361 agtaccagtg gcgccgcgag ggcgagtacc acctgttcaa cccggagacg gtgttcaagc
     9421 tgcagcacgc cacccgcacc gggcagttcc agatcttcag ggagtacacc cgcctcgtgg
     9481 acgaccagag cgagcggatg gcgtcgctgc gcggcctgct caagttccgc gccgacgtgc
     9541 ggcccgcggt tccgctggac gaggtcgaac ccgccagcga aatcgtcaag cgcttctcga
     9601 cgggagcgat gagctacggc tcgatctccg cggaggcgca cgagacgctg gccatcgcga
     9661 tgaaccgctt gggcggccga tccaacagcg gcgagggcgg cgaggacgtc aagcgattcg
     9721 aacgcgaccc caacggggac tggcgccgca gcgcggtcaa gcaggtcgcc tcggcccgat
     9781 tcggcgtcac ttcgcactac ctgaccaact gctcggatat ccagatcaag atggcccagg
     9841 gtgctaaacc cggtgagggc ggccaacttc cgggacacaa ggtgtacccg tgggtggccg
     9901 aggtccggca ctccacccct ggtgtgggcc tgatctcgcc gccgccgcac cacgacatct
     9961 actccatcga ggatctcgcg cagctgatcc acgacctgaa gaacgccaac ccgtccgcgc
    10021 gggtgcacgt caagctggtc tccgagaacg gggtggggac ggttgcggcc ggagtgtcca
    10081 aggcgcacgc cgacgtggtg ctcatctcgg gacacgacgg gggcaccggc gcaacgccgc
    10141 tgacatcaca gaagcacgcg ggtgcaccgt gggagctggg gctggccgag acgcagcaga
    10201 cgttgctgct caacggattg cgtgaccgga tcgtggtcca ggtcgatggt cagctcaaga
    10261 ccggccgcga tgtcatcatc ggcgcgctgc tgggcgccga ggagttcggc ttcgccaccg
    10321 cgcctttggt ggtggtcggc tgcatcatga tgcgggtgtg ccacctggac acctgcccgg
    10381 tcggcgtggc cacccagaac cccgtgctgc gggagcggtt caccggcaag cccgaattcg
    10441 tcgagaactt cttcctcttc atcgccgaag aggtccggga atacatggcg gagctgggct
    10501 tccggacgtt caacgaaatg gtcggccagg tcgggtcgct ggacaccacg ctggcgcgcg
    10561 cgcactggaa ggcgcacaag ctggacctga cgccggtgct gcacgagccg gagtcggcct
    10621 tcatgaacca ggacctgtac tgcagctcgc gtcaggacca cggcctggac aaggcgctcg
    10681 atcagcagtt gatcgtgatg agccgcgagg cgctggactc cggttcacct gtccgttttt
    10741 cgacggcgat cagtaacgtc aaccgcacgg tgggcaccat gctcggccac gagctgacga
    10801 aagcctatgg cggccaaggt ctaccggacg gcaccatcga catcaccttt gaaggatcgg
    10861 cgggcaacag cttcggagcc tttgtgccgc gggggatcac gctgcgtgtc tacggcgacg
    10921 ccaacgacta cgtcggcaag ggactatccg gaggccggat cgtggtgcgg ccgtcggaca
    10981 acgcgccgca ggactacgtc gccgaggaca acatcatcgg cggcaacgtg attctgttcg
    11041 gcgccaccag cggggaggtc tacctgcgcg gtgtggtggg cgaacggttc gctgtccgca
    11101 actccggggc acacgcggtg gtcgagggcg tgggcgatca cggctgcgaa tacatgaccg
    11161 gtggcaaggt ggtcatcctg ggccgcaccg gccgcaactt cgcggcgggc atgtccggcg
    11221 gtgtggccta tgtgtacgac ccgcacgaag aactgccgga aaacctcaac gaggaaatgg
    11281 tcgaactcga gaccctcgac gaagacgacg cagacttcct gtacgggacc atccaggccc
    11341 acgtcgacgc caccgattcg gctgtcggcc agcggatcct ggccgactgg cgcgggcagc
    11401 aacggcactt cctcaaggtg atgccccggg actacaagaa ggtgctgcag gccatcgccg
    11461 aagcggagcg tgacggcgtg gatattgaca aggcgatcat ggcggccgcg catggctgat
    11521 cccaccggct tcctgaaatt cactcatcgg gaactgccgc agcgccggcc tgtcccgctg
    11581 cgactgcgcg actggaacga ggtctacgag gaattcgatg accacaccct gcgccaccag
    11641 gcgacccgtt gcatggattg cggtattccc ttctgccaca acgggtgtcc actgggcaac
    11701 ctgattccgg aatggaatga cctggtgcgc aggggtcgtt ggcgcgatgc gatcgaacga
    11761 ctgcacgcca ccaacaattt cccggacttc accggccggc tgtgtccggc cccgtgtgag
    11821 ccggcgtgcg tgctcgggat caaccaggac ccggtgacga tcaagcagat cgagctggag
    11881 atcatcgatc acgccttcga cgaaggcttt gtggtgccgc tgcccccgga ccggctgacg
    11941 ggaaagactg tcgccgtggt gggttcggga ccggcgggcc tggcggctgc ccagcagttg
    12001 acccgcgccg gccacagcgt gaccgtcttc gagcgcgacg accgcatcgg tgggctgctg
    12061 cgctacggca tcccggaatt caaaatggaa aagcgcatcc tggaccgacg gctggatcag
    12121 atgcgggccg agggcaccga attccggccc agcgtcaacg tcggggtcga catcaccgcc
    12181 gaacagctgt gcgccgattt cgacgcggtg gtgctggccg gcggcgccac ggcctggcgc
    12241 gatctgccca ttcccggtcg ggagttggac ggcatccacc aggcgatgga gtacctgccg
    12301 tggggcaacc tgcaagcgct ggatgagctc gacgccgcag acctggacga ggacggggag
    12361 ccgccgatca ccgccaaagg caaaaaggtc gtcatcatcg gcggcgggga caccggagcc
    12421 gactgcctgg gcaccgtgca ccggcagggc gcgaccgagg tgcaccagtt cgagatcatg
    12481 ccgcgtccgc cggacgcgcg cgccgaatcc accccgtggc cgacctaccc gctgatgtac
    12541 cgggtgtcgg cggcgcacga agagggcggc gagcgggtgt tctcggtcaa caccgaggaa
    12601 ttcgtcggca aagacgggca cgtgaccgct ctcaaggtgc acgaagtcac catgcgcgac
    12661 ggcaggttcg tcaaggtaga gggctccgat ttcgacctcg aggccgacct ggtgttgctg
    12721 gcgatgggat tcgtcggtcc ggagaagccg ggcctgctca ccgacctcgg agtggacttc
    12781 accgagcgcg gcaacgtcgc gcgcggcgcc gatttcgaca cctcggtgcc cggtgttttc
    12841 gttgctggcg acatgggtcg tggccagtca ctgatcgtct gggcgatagc tgagggcagg
    12901 gccgcagcgg cgggcgcaga ccgctatctg atggggtcga cggcactgcc ggcgcccatc
    12961 aagccgacgg ccgcaccgct tcagtaatca caccagtcac accagaaaca tggctgtagt
    13021 cgaccggcgt ttctcccgcc gtttgccaga cgcctgttgt ctaatgatcc gttgtgggcc
    13081 ccatcacata gcaatcacgt gggagtcccg ttgaacggac cgatcgcagg agtgatcact
    13141 gtgcaaatca aaccattatc tcggtcatgg atggggcgaa tcgcctggtc atggtttcgc
    13201 cacccgatga agagccgcga gtttcccgcc aagcacgagc gcctggtaac cgccgaagaa
    13261 ctgatggctt tcggcgtcta acagcgcctg cgggcgtgtc cgcggcatcc aaaagatttg
    13321 ctcagcgttt gcctgggcgt tatccctgcg agaaacccta aattcctcca ctatcactga
    13381 ctccatagcc ccgaatcccg gatcgcttcg gccaagggtt ccaatacggc cggatcgtgc
    13441 ggcggtgcga tgtagacgat gcccaggtcc aggccttcgg cggccagggc ggccgcgtcc
    13501 tcgaggactt tcccataatt gcggtccgcc tccaggcgaa ggtgggcgga cttcgtgatc
    13561 tccttggggt cgcgtccgat gtctgcgcaa cgcgcggcca acacgtcgcg cttctgcgcg
    13621 aacagctcgg gtgggccgcc ggcgaagttc cagtgctggg cgtagcgcgc ggtgatcggc
    13681 aatgtgcgtt tctcgccgct gccgccgatg cagatcggcg ggtgcggccg ctgaggtccc
    13741 ttgggctcgt tgcgggcgcc cttgagctgg tagtacttgc cgtcgaagtc ggtggtctcc
    13801 tcgctcagca ggctgaccag cacctggcag gcttcctcga accggtcgaa gcgttccttg
    13861 atggtgccca actcgatgcc gtaggcgccc gactcctctt cattccaccc cgcgccgatg
    13921 cctagctcga gtcgcccgtt ggagatgatg tcgaccgcgg cggccatatt ggccagcacc
    13981 gccgggtgcc ggtaatggat gccggtgacc agggtgccca accgcagtcg cgtggtggcc
    14041 tgcgccagcg cggtcaacgt cgtccagccc tccaggcagg gcccgctgct gtcggtaaag
    14101 atcggataga ggtggtcgaa ggtccaacct gagtcgaaga cgtcgatgtc gtcggccgcc
    14161 cgccaaacgt cgagcatctg tgcccaggtg gtgttttggg gtgaggtttt gaacgcgaat
    14221 cgcatgaact cgacgctagt ccaactttat taagcgcagc taaacgcatc cggacaatga
    14281 ctagcgcgct cggccctcgg tctcgatgtg ttgtgacccg gttaggcaca ctggagcgat
    14341 ggatccggtg accgcgttgc ggcagatcgc ctattacaag gaccggagcc gccaggaccc
    14401 caagcgtgtg atggcgtatc gaaacgctgc cgacatcgtc gagggtctca gcgacgcgga
    14461 gcgagagcgg cacgggcagg ccaacagttg gcagacgttg gtggggatcg ggcccaaaac
    14521 cgcaaaggtc atcgaccagg cctggtccgg tcgcgaaccg gacgccctgg tcgaattacg
    14581 cgatgccgct gaggatctcg gtggcggcga tgtccgtgcc gcgctgcgcg gcgacctgca
    14641 cctgcattcg aactggtcgg acggctcggc gccgatcgag gagatgatgg ccactgccgc
    14701 cgcgctcggg cacgagtact gcgcgctgac cgaccactcg ccgcggctga ccgtcgccaa
    14761 cgggctgtcg ccggagcggc tgcgcaggca gctcgacgtc atcgacgggt tgcgcgaccg
    14821 cttcgcgccg atgcgcattc tcaccggcat cgaggtcgac atcctcgacg acggcagcct
    14881 ggaccaggaa ccggagctgc tggagcgcct cgacgtcgtg gtggccagcg tccattcgaa
    14941 gctgtcgatg gattccgcgg cgatgacacg gcgcatggtg cgcgccgtgt ccgacggcca
    15001 cgccgacgtt ctgggccact gcaccggtcg actggtcgcc ggtaaccgcg gcatccggtc
    15061 ggaatcgaag ttcgacgccg aaaaggtgtt caccgcctgt cgtgaccatg ggaccgcggt
    15121 ggagatcaac tcccgtcccg agcgccgcga cccgccgacc cgtctgctca agctggcgct
    15181 cgagatcggc tgtgtgttca gcatcgacac cgacgcgcac gcgcccggcc agctggactt
    15241 cctcggttat ggcgctcagc gcgcgctgga cgccggcgtg cccgtcgacc ggatcgtcaa
    15301 cacctggccc gccgaaaagc tgctgacctg gaccgcgtct tgaggctggc tgcgccgacg
    15361 gaggactgtc cgctgtgccc gccagcaacg tcgggactcg tcaaacgatt acaactggat
    15421 tccagagtga cccctcgaat gggcagtggc ccgaatgtcc agtaaggggg tactccaggg
    15481 tgtaagacca ggcaattgag tgcttagttg cgtgaggtca cacccaggtc gtgggagaac
    15541 acaactacac aaggccgggt acgaacgtca cccgccgccc ggtcctagtc aacggccacg
    15601 tgatacttag cctgacttat cagggtcagc aggtactcgc ctactctagc cggcggcgaa
    15661 tcccggatgt ttacattctc gacgagagca tatgaccacc aggtcacacc gggcagagag
    15721 gcggcagctc atgaccacga ggggcaagta cacggacgtg cccgcgccgt actcgtggga
    15781 agtccccagc gccggcgaga cgcgcttcac gtgggagtac gacgaggggc gcgcccggct
    15841 cctttcgctg taccaaaagg gaaaggacaa gcagtgggat gcccagtcgc gcatcgactg
    15901 gtcgcaagac gtcgacccga tgaacccggt cggcctgccc gacgagttcc atccgctgtt
    15961 cggcagcccg atgtgggacg ccgctgacga atcacgtcgc gccgagatgc gccagcactc
    16021 tcaggcctgg cagttctcgc agttcctgca cggcgagcaa ggggcgatgg tgtgcgcggc
    16081 caagatcgtc gaggtggtcc cggacctgga cgcgaagttc tacgcggcca cccagaccat
    16141 ggacgaggcc cgccacgtcg aagcgttttc gcggttcctg caggagaagg tcgacctggt
    16201 ctacccgatc aacaagaacc tcacagcact gctggaggac accctgcgcg actcgcgctg
    16261 ggacatgcca tacctcggca tgcaggtgct catcgaaggg ctcgcactcg ccgccttcgg
    16321 gacattgcgt gacttggcgc cgcaggactc actggccaag cagttactgg cctacgtcat
    16381 gcaggacgag gcccgccacg tcgccttcgg ccgaatctcg ttgaaggact actactccgc
    16441 gttgaccgag gccgagcggg acgagcgtga agagttcgtc gtggacgctt gctacctgat
    16501 gcgcgaccgg ttccgcggcg aggaggtttt cgagaccctc ggcatgaatg tccaagagtg
    16561 tgcagagtgg gtcgacacgt cgccggtgat gatccagttc cgctcgcacc tgttcagccg
    16621 catcgtgccg atcgtcaagg acatcgggtt gtggggcgac aaggtgcaga aggccttcag
    16681 ggacatgggt gtccacgaca tgggcggctt cgacatcgag gcgctgatga aggccgatga
    16741 ggatcaagcc gacgcgctgg ataagacaca cgccgacatg gccgaccgag cactcgaggt
    16801 ggaccaggtg atcgcggcgg gcgcgagcta accgttgccc tttctcagca gaatccgccg
    16861 gaaccaacaa gcggcggata gggcccccgc gttcatcaca ggcgtaaccg gttgggggtc
    16921 aacgagaaac gcactgagag caggggggcg tcgacacaac gcacctgcat ggacaccgcg
    16981 gaaaaccctg gccaggcaca gcaccagcac cacgaccagc gctgcgatcg ccaatgctgc
    17041 ttcaacggtg cttgcagccg cacaggcggc tacaccttat tgagcgcgcg accgatgatg
    17101 ttggtcagcg ccgacacgat ggaatcgccg gtgacgaccg tgtagacgac cacaccgaag
    17161 gcggccgcgg cgaaagtgcc gatggcgtag tcaactgtcc ggttttacac ctcgggcctt
    17221 gttggtgtcc gtcctgtgca tctcgccgtt tggcggtatc cgaacgtcga ttcgacctat
    17281 cggcgaccag atactggacg agaatgaagc gggtgatccg aggtcgaaca agacccttaa
    17341 ccgagggaag tgacgagaat gaaccgcttc gacatcaccc agaccaacgt cgctgtggag
    17401 cgactgattg agacgacgga gaacccacgc cacctgtatc tactccatgc ttacaaccgc
    17461 caccgctacc ttgagatggc cggccgcttc gaggagatct tcacacctga aatgatgacc
    17521 gag
//
LOCUS       DASSGQ010000242         4841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_503401,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000242 DASSGQ010000000
VERSION     DASSGQ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503401"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="VFI55_10695"
     CDS             <1..1309
                     /locus_tag="VFI55_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HET7074532.1"
                     /translation="MWVRGFGADYDEWGKRAGPHWSYAEAVECFRRIEDVTAAWHSVS
                     GDDSGVTGPLSLSRQRSPRPSTAAWLAATRQCGFPPAKPNSAAPEGFCETVVTQRRGA
                     RCSTADAYLKPAMGRKNLTVLTEATATRVVFVGNRAAGVEYERDGQRSVAYARREVVL
                     CGGAINSPQLLMLSGIGDRDHLAEHGIDTLYHAPEVGQNLLDHLITLLGFDVNDDSLF
                     AGEKPKQLINYLLRRRGMLTSNVGEAYGFFRSRPELDLPDLELIFAPAPFYDEALAAP
                     QGHGVVFGPILVAPESRGQITLRSADPHDKPVIEPRYLSDPGGVDRAAMMEGLRACAR
                     IAEAPPLKGMLGRLARPRDCIELNEETLELALTNCSHTLYHPLGTCRMGSDDSSVVDP
                     QLRVRGVDSLRVADASVMPSTIRGHTHAPSVLIGEKAAELILA"
     gene            1405..2676
                     /locus_tag="VFI55_10700"
     CDS             1405..2676
                     /locus_tag="VFI55_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009955263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7074533.1"
                     /translation="MTMNAKRRRVHTKLAALPGVRPVRRPISPDGHHRGAGDGQEFDL
                     YYVRGGRKSAHPLVIIPGGPGVASVQMYRGLRRRAALAGLDVIMIEHRGVGMSRHDDS
                     GADLPPHALTVDQVVDDVAAVLDDAQVGSAVIYGTSYGTYIAAGLGVRHPGRVRAMIL
                     DSPLLSHHDIVIVRHAIRRLLLRGDTSETASLASKVRRLLDTGVMTASATQVIATVYG
                     YGGTALLDRQLDLLLDGRKMLWHALSRFTTIGKVPYRYEEDLVSRIALRELDYAAEPD
                     GLPLDPAVTERETRTQKATFEDEPYDLVAEMPNFSWPTVVICGGRDLITPPAVAERIA
                     SLVPNAVLLKLPTMAHSALDFREPAALSIACAVWRGEFEGLAARAPALDALPARAAVR
                     LLWKAVEVAAIAESALPALTAVAPLRRLRSA"
     gene            complement(2663..4441)
                     /locus_tag="VFI55_10705"
     CDS             complement(2663..4441)
                     /locus_tag="VFI55_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter family substrate-binding
                     protein"
                     /protein_id="HET7074534.1"
                     /translation="MASRSTRRVCGHVESALTAPFRRESSPWTRTRRYAGRVATVLIV
                     IAIVATGCSPAINLAPSAGNNARIGTTSDVNPQDPATLRDGGDLRLALSDFPPNFNIL
                     HIDGNSAEVAAMMKATLPRAFIIGPDGSTTVDTDYFTSVELTGTNPQVVTYTINPKAV
                     WSDGTPITWRDIASQINALSGVDKNFAIAGPGGAERVASVTRGIDDRQAIMTFAKPYA
                     EWRGMFAGNGMLLPASMTATPEAFNTGQLKGPGPSAGPFVVSSLDRTTQRIVLTRNPK
                     WWGRQPRLDTITYLVLDDAARLPALQNNTIDATGIGTVDQLTIAERTKGISIRRAPAP
                     SWSHFTFNGAHGSILEDKALRVAVSMGIDRQTIAKVVQYGLTSNPVALNNHVYVAGQE
                     GYQDNSAILPYDPAEARRELDALGWKLSSQFGGQFREKDGRQLVIRDLFYDAQGSRVF
                     AQIAQHSLAQIGVKLELVARAGSGFFTNYINVGAFDIAQFGWLGDAFPLSALTQIYKS
                     DGESNFGKIGSPEIDSAIEQTLEELDPEKARALANDVDKLIWAEGFSLPLTQSPGDVA
                     VRGSLANFGAPGLADLNYTAIGFMRT"
     gene            complement(4434..>4841)
                     /locus_tag="VFI55_10710"
     CDS             complement(4434..>4841)
                     /locus_tag="VFI55_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7074535.1"
                     /translation="RVAELLDIVGLRRADATRYPAEFSGGQKQRIGIARALALQPKIL
                     ALDEPVSALDVSIQAGIVNLLLDLQERFGLSYLFVSHDLSVVKHLAHRVAVMYAGTIV
                     EQGDSQQVFSNPQHDYTRRLLGAVPQPDPGTYG"
BASE COUNT          807 a         1567 c         1621 g          846 t
ORIGIN      
        1 gatgtgggtg cgcgggttcg gcgcggacta cgacgagtgg ggtaagcgcg caggccccca
       61 ttggtcctac gcggaggcgg tcgaatgttt tcgtcgcatc gaggacgtca ccgcggcgtg
      121 gcattccgtc agcggcgacg acagcggagt taccggaccg ttgtctcttt cccgtcagcg
      181 gagtccacga ccttccacgg cggcgtggct cgctgcgaca cgccagtgcg gcttcccgcc
      241 tgcgaaaccg aattccgctg cgccggaagg cttctgcgag accgtcgtca cacagcgccg
      301 cggcgcccgc tgcagcaccg ccgacgcgta tttgaaaccg gcgatgggac gcaaaaacct
      361 cacggtgctc accgaggcaa ctgccacccg agtcgtcttc gtcggcaacc gggcagcggg
      421 cgtggaatac gagcgggacg gccaacgaag cgtcgcctat gcccgccgcg aagtggtgct
      481 ttgcggcggt gccatcaaca gcccgcaact actgatgctc tccggaatcg gcgatcgcga
      541 tcacctcgcc gaacacggca tcgacaccct ctaccacgca cctgaggtgg ggcagaatct
      601 gctcgaccac ctcatcacgt tgctcggctt cgatgtcaac gacgacagct tgttcgccgg
      661 cgagaagccg aaacagttga tcaactacct gctgcggcgc cgaggcatgc tcacctccaa
      721 cgtcggtgaa gcgtacggat tcttccggag ccggcccgag cttgacctgc ccgacctcga
      781 actgatcttc gcgccggcgc ccttctacga cgaggcactc gccgcgccgc aaggacacgg
      841 tgtggtgttc ggcccgatct tggtcgcgcc ggaaagccgc ggccaaatca cgctgcgctc
      901 cgcggatcca cacgacaagc cggtgatcga accgcgttac ctctcagatc cgggcggtgt
      961 cgaccgtgcc gcgatgatgg aagggcttcg ggcctgcgcc cgcatcgccg aggccccccc
     1021 gttgaaaggc atgctcggcc ggctcgcgcg accgcgtgac tgcatcgagc tcaacgagga
     1081 aaccctggag ttagcactca ccaactgttc gcacacgctg tatcaccccc tgggcacctg
     1141 ccggatgggc agcgatgact cgagcgtggt ggatcctcag ctgcgggtcc gcggggttga
     1201 cagcctccgg gtagccgacg cctcggtgat gcccagcacg attcgtgggc acacccacgc
     1261 gccatcggtg ctgatcgggg agaaggcagc cgagctcatc ctggcctgac cggcgcctat
     1321 cggcgaccat gccacaggtc gtccggcttg cccggccgat tgggcgccgt ctcggcagaa
     1381 tgggaccatg gcggggcgac gtcgatgacg atgaatgcga aacgacgccg ggtgcacaca
     1441 aagttggcag cgttgcccgg ggtgcgtccg gtacggcggc cgatctcgcc ggacgggcac
     1501 catcggggtg cgggggacgg tcaagaattc gatctgtact acgtgcgcgg cgggcgcaag
     1561 tccgcccacc cgctggtgat catcccggga ggcccgggag tcgcgtcagt gcagatgtac
     1621 agagggctgc ggcgccgggc tgcccttgcc ggcctcgacg tcatcatgat cgagcatcgc
     1681 ggcgtgggaa tgtcgcgcca cgacgactcc ggcgccgacc tgccgccaca tgcgctcacc
     1741 gtcgatcagg ttgtcgacga cgtggcggcg gtgctcgacg acgcccaggt gggctcggcc
     1801 gtcatctatg gcacgtcgta cggcacctat atcgcggccg ggctcggcgt acggcatccc
     1861 gggcgggtgc gggccatgat cctcgattcg ccgctgctgt cacatcacga catcgtgatc
     1921 gtgcgtcacg ctattcgccg gctgttgctg aggggtgata cctccgaaac cgcctcactg
     1981 gcaagcaaag tccgcagact tctcgatacc ggtgtgatga ccgcgtccgc gacgcaggtc
     2041 atcgcgacgg tctacggcta cggcggcaca gctctgctcg atcgtcaact cgacctgttg
     2101 ctcgacggcc gaaaaatgct gtggcacgca ctatcccgtt tcaccaccat cggcaaggtg
     2161 ccgtaccggt acgaggaaga cctggtgagc cgcatcgcgt tgcgcgaact cgactacgcc
     2221 gccgaacccg atggcctgcc actggacccc gcggtaaccg aacgcgaaac acgcacccag
     2281 aaggctactt tcgaggacga gccgtacgac ctggtggcag agatgccgaa tttcagctgg
     2341 cccaccgtgg tgatttgtgg cggccgcgac ctcatcaccc cgccggcggt ggccgagcgg
     2401 atcgcgtcgt tggtgcccaa tgcggtgttg ttgaaactcc ccacgatggc gcacagcgcg
     2461 ctggatttcc gcgagcccgc cgccctgtcc atcgcctgtg cggtgtggcg cggcgaattc
     2521 gaagggcttg ccgctcgagc accggcgctg gacgcgctcc cggcgcgggc ggcagtgcgt
     2581 ctgctgtgga aagccgtcga ggtggctgcg atcgcagaaa gcgccctgcc ggccctgacc
     2641 gcggtggcac cgctgcggcg gctcaggtcc gcatgaaacc gatcgcggtg tagttcaggt
     2701 ccgcgagacc gggtgcgccg aaattggcga gtgagccgcg caccgcgacg tcgcccggag
     2761 actgggtgag cggcaggctg aaaccctccg cccagatgag cttgtcgacg tcgttggcca
     2821 acgcccgtgc cttttcggga tcgagttcct cgagcgtttg ctcgatcgcg gagtcgatct
     2881 ccgggctgcc gatcttgccg aaattgcttt ctccgtcgga cttgtaaatc tgggtcagcg
     2941 ccgacagcgg gaacgcatcg ccgagccaac cgaattgcgc gatatcgaaa gccccgacgt
     3001 tgatgtagtt ggtgaagaag ccgctgcctg cccgggccac cagctcgagc ttgaccccga
     3061 tctgggcgag gctgtgctgc gcgatctgtg cgaacacccg gctgccctgc gcgtcataga
     3121 acaaatcgcg gatgacaagc tggcgaccgt ccttctcccg aaactggccc ccgaactggc
     3181 tactgagctt ccagcccagg gcatcgagtt cgcgccgcgc ctcggccggg tcgtagggga
     3241 ggatggcgct gttgtcctga tagccctcct gcccggccac gtagacgtgg ttgttcaacg
     3301 ccaccgggtt gctggtgagg ccgtactgga ccaccttggc gatggtctgc cggtcgatgc
     3361 ccatggagac ggcgacccgc agcgccttgt cttccaggat tgagccgtgc gcaccgttga
     3421 acgtgaagtg agaccagctc ggggcggggg cgcggcggat cgaaatgccc ttggtgcgtt
     3481 ccgcgatggt cagctggtct accgtgccga ttccggtcgc gtcgatggtg ttgttctgca
     3541 gcgcgggcag ccgcgcggcg tcatcgagca ccaggtaggt gatggtgtcc aggcgcggtt
     3601 gcctgcccca ccatttgggg ttgcgggtca acacgattcg ctgcgttgtc cggtccagtg
     3661 aagaaacgac gaacgggccg gccgacggac ctggcccctt gagctggccc gtgttgaacg
     3721 cctcgggcgt ggcggtcatg ctggcgggca acagcatgcc attgccggcg aacatgcccc
     3781 gccactcggc gtacggcttg gcgaaggtca tgatggcctg ccggtcgtct atcccgcggg
     3841 tcaccgatgc gacccgctcg gcgccgcccg gtcctgcgat cgcgaaattc ttgtccacgc
     3901 cgctgagagc gttgatttga ctggcgatgt cgcgccaggt gatcggtgtc ccgtcggacc
     3961 acacggcctt cgggttgatg gtgtaggtga ccacctgtgg gttggtcccg gtgagttcga
     4021 cgctggtgaa gtagtcggtg tcgacggtcg tcgagccgtc cggcccgatg atgaacgccc
     4081 gcggcaacgt ggccttcatc atcgcggcaa cctcggccga gttgccgtcg atatgcagaa
     4141 tgttgaaatt cggtggaaag tcgctgaggg cgagccgcag gtcgccgccg tcccgcagcg
     4201 tggcgggatc ctgtgggttg acatcgctgg tggtgccgat ccgcgcgttg ttgccggccg
     4261 agggcgccag gttgatggcc ggcgagcaac cggtagccac gatggcaatg acgatgagca
     4321 ctgtcgcaac ccggccggcg tagcgccgag tccgcgtcca cggcgaggat tctcggcgaa
     4381 acggcgccgt gagcgcagac tcgacgtgac cgcagactcg acgtgttgat cggctagcca
     4441 taggtgcccg gatctggttg cggcaccgcc cccagcagcc gccgggtgta atcgtgctgc
     4501 gggttactga agacctgctg gctgtcgccc tgctcaacaa tggtgccggc gtacatcacc
     4561 gccacccggt gggcgaggtg tttgaccacc gaaaggtcat gagacacaaa cagatacgac
     4621 agaccgaacc gttcctgcaa gtcgagcagc aggttgacaa tcccggcctg gatcgatacg
     4681 tcgagggcgg acacgggttc gtcgagagcc agaattttgg gctgcaatgc cagcgcccgc
     4741 gcgataccga tgcgttgctt ttgcccgccg gagaactcgg cggggtagcg ggtcgcgtcc
     4801 gcgcgccgca atcccacgat gtcgagcagc tcggcgactc g
//
LOCUS       DASSGQ010000243        11858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1262374,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000243 DASSGQ010000000
VERSION     DASSGQ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11858
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262374"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VFI55_10715"
     CDS             complement(<1..140)
                     /locus_tag="VFI55_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPE domain-containing protein"
                     /protein_id="HET7074536.1"
                     /translation="MFDFAALPPEINSGRMYAGPGSESMMVAATAWDELAAELGTAAS
                     GY"
     gene            complement(640..834)
                     /locus_tag="VFI55_10720"
     CDS             complement(640..834)
                     /locus_tag="VFI55_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET7074537.1"
                     /translation="MKVRLERSKCVGHAQCYAVDPDQFPIDEAGNSILEEHEVRPEDE
                     QVIRDGVASCPEMALIIDGE"
     gene            1150..2325
                     /locus_tag="VFI55_10725"
     CDS             1150..2325
                     /locus_tag="VFI55_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7074538.1"
                     /translation="MSSVGGGQPGTFYLPRLEYSKLPLGADRGVGWKTLRDAGPVVFM
                     NGHYYLTRREDVLAALRNPKVFSSRLALQPPGSPLPVVPLAFDPPEHTRYRKILQPYF
                     SPQGLSESRPVLQRHATEMIANFAPRGECEAMADFARIYPFQVFLDLYGLPLEDLDRV
                     IAWKDAIVADKPYMTREDLDKGHELLAYLSDAIQQRRQNPGSDMLSRVMYGDGDFSDL
                     ELLGMSHLLILAGLDTVTAAIGFSLFELARRPELRHALRDNPKQIRVFIEEIVRLEPS
                     APVAPRVTTEIVTVGGMTLPAGTHVRLCMAAVNRDDSDAMSTNDLVMDGKVHRHWGFG
                     GGPHRCLGSHLARIELTIIVAEWLKQIPDFELPPGYSPEIKFPSKTFALKELPLHWD"
     gene            complement(2399..6571)
                     /gene="eccCa"
                     /locus_tag="VFI55_10730"
     CDS             complement(2399..6571)
                     /gene="eccCa"
                     /locus_tag="VFI55_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_007410461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccCa"
                     /protein_id="HET7074539.1"
                     /translation="MKRGFARPTPEKAPVIKPENIVLPTPLSIPPPEGKPWWLIVVGV
                     LVVGLLIGMVAMTFASGSHVFGGAGSIFPIFMIGGVAMMMFGGRMGGGQQQMSRPKLD
                     AMRAQFMLMLDMLRETAHESADSMDANYRWFHPSPDTLPAAVGSSRMWERKPDGKDLN
                     FGVVRVGVGMTRPEVTWGEPQNMPTDIELEPVTGKALQEFGRYQSVVYNLPKMISVLV
                     EPWYSLIGDREQVLGLMRAIVCQLTFSHGPDHMQLIVVSSDLEQWDWVKWLPHFGDQR
                     RQDAAGNARMVYSSVREFAAEQAELFAGRGSFTPRHASSSAQCPTPHTVIIADVTDPQ
                     WEYVISAEGIDGVTFFDLTGGSMWSTVGERTLRFDERGVIEALPRDRDTWMVIDEKPW
                     FFALTDHLSAVEAEEFAQKLARWRLAEAYEEIGQRVAHLGARDILSYYGIDDPSDIDF
                     DALWGSRTDTMGRSRLRAPFGNRSDNGELLFLDMKSLDEGGDGPHGVMSGTTGSGKST
                     LVRTVIESLMLGHPPEELQFVLADLKGGSAVKPFSGVPHVSRIITDLEEDQALMERFL
                     DALWGEIARRKAVCDNAGVDDAKEYNSVRSRMRARGQDMPPLPMLVVVIDEFYEWFRI
                     MPTAVDVLDSIGRQGRAYWIHLMMASQTIESRAEKLMENMGYRLVLKARTAGAAQAAG
                     VPNAVNLPAQAGLGYFRRSLEDIVRFQAEFLWRDFYARGITMDGQEAPMLAHSIDYVR
                     PQLFTNSFTPLEVSVGGPDIEPVAVHSNGEVLEVEDPDQVEDEDDAIRTPKVGMEIIH
                     QLRRIDFEPYRLWQPPLTQPVAIDELVNRFLGHPWQKDYGSAKDLVFPIGIIDRPFKH
                     DQPPWTVDTSGPGANVLILGAGGSGKTTALQTLICSAAMTHTPEQVQFYCLAYSSTAL
                     TTVAGLPHVGEVVGPTDPYGVRRTVAELLALVRDRKRTFLEYGIASMEVFRRRKFGGA
                     PGPVPDVFGDVYLVIDNYRALAEENEVLIEQVNLIINQGPSFGVHVIVSADRESELRP
                     PVRSGFGSRVELRLAAVEDAKLVRSRFAKEVPVKPGRGMLAVNYVRLDADPQAGLHTL
                     VARPALGGTPNNEFESDSIVAAVSRVASGQARPVRRLPARFGVQQVRELAARDTRQGV
                     GVGGIAWAISELDLEPVYLNFAENAHLMVTGRRECGRTTTLATIMSEIGRLYAPGATG
                     APPPPPGRPSAQVWLVDPRRQLLTVLGREYVEKFAYNLDGVQAMMGELAAVLAGREPP
                     PGLTAEELLSRSWWSGPEIFLIVDDIQQLPAGFDSPLHKAAAWVTRAADVGLHVLVTR
                     TFGGWSSAGSDPMLRSLHQANAPLLVMDADPDEGFIRGKMKGGPLPRGRGLLMAEDTG
                     VFVQVAATEFRK"
     gene            complement(6568..8088)
                     /gene="eccB"
                     /locus_tag="VFI55_10735"
     CDS             complement(6568..8088)
                     /gene="eccB"
                     /locus_tag="VFI55_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccB"
                     /protein_id="HET7074540.1"
                     /translation="MAEESRGGRGSGYGLGLSTRTQVTGYQFLARRTAMALTRWRVRM
                     EVEPGRRQTLAVVASVSAALVICLGALLYSFISPSGQINESPIIADRDSGALYVRVGD
                     RLYPALNLASARLITGRPDNPHLVRGSQIAGLPRGPLVGIPGAPSNFATKTPPASSWL
                     VCDTVGTSMGLGAPSGVTVTVIDGTPDLSGHRQVLNGSDAVVLSYGGDAWVIRQGRRS
                     RVDATNRSVLLPLGLTPEEVEMAKPMSRALFDALPVGPELTVPDVPNAGAAAAFSGAP
                     GAVGTVLVTPQISGPQQYSLVLTDGVQTLPPLVAQILQNAGGPGNTKPVTVEPAALAK
                     MPVVNKLDLSSYPDNPLNVMDIRENPATCWWWQKTSGENRARVQVVSGSTIPVTQKDM
                     AKVVSLVKADTTGRQADQVFFGPDYANFVAVTGNDPGAKTTESLWWLTDAGARFGVDD
                     TREVREALGLKTAPSLAPWVALRLLPQGPTLSRSDALVEHDTLPMDMSPAELVVPK"
     gene            8537..10684
                     /gene="malQ"
                     /locus_tag="VFI55_10740"
     CDS             8537..10684
                     /gene="malQ"
                     /locus_tag="VFI55_10740"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET7074541.1"
                     /translation="MTELAPSLVELARRFGIATEYDDWTGRRVLVSEITLVAVLAALG
                     VAGGTEQERNAALTAQLRSYWARRMPPTIVGRTGVQTRFWVHVTHGHPAEVWLQLEDG
                     TMRGGLQQVDNFTPPFDLDGRWVGEATFVVPADLPLGYHRVHLRSGDAETSTALIVTP
                     DWLGLPEQLGARRAWGLAVQLYSVRSRQSWGIGDLTDLTDLAVWSASRHGADYLLVNP
                     LHAAGVSGPTNRMEPSPYLPTSRRFVNPLYLRVEAVPEFAELTKRSRVRRLRADVQRD
                     AAKLDAIDRDSAWAAKRAALKLLHAEPRSAGRELAYSAFRDREGRALDDFASWCALAE
                     EYGGDWHRWPESLQHPDAAGVAGFVERNSDTIDFHRWLQWQLDEQLASAQSQAIRAGM
                     ALGIMADLAVGVHPNGADAWALQDVMARGVKAGAPPDEFNQLGQDWSQPPWRPDRLDE
                     QEYRPFRALIQAVLRHAGGVRIDHIIGLFRLWWIPDGAPPTQGTYVRYDHEAMIGIVA
                     LEAHRAGALVVGEDLGTVEPWVRDYLLLRGVLGTSILWFELDRDGTGGPLPASRWREY
                     CLSSVTTHDLPPTAGYLVGDHVLLRESLGLLTRPVEIELESDRAELAGWMAELRRVGL
                     LDDGEDDPDQIVLALYRYLGRTPSRLLGVALTDAVGDHRTQNQPGTTDEYPNWRVPLT
                     GPDGRQVMLEDVFTDRRAATLAEAVRTEIAPRG"
     gene            10710..11591
                     /locus_tag="VFI55_10745"
     CDS             10710..11591
                     /locus_tag="VFI55_10745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET7074542.1"
                     /translation="MKVCVVAPVADGVTADPDWMVSFARHLERCGFESIVVVEHTVLV
                     TRYDSVYPYDSSGRVGLAADCSIPDPLDLLAFLAGHTTRLGLATGVLVLPNHHPVVLA
                     KRAATVDALSGGRLRLCVGVGWLQEEVEACGAEFDSRGRRADEQLAVLRTLWADQPRG
                     ASYHGEFFGFDDVMCYPKPVAGERLPIHIGGHSRAAARRAGRFGDGFQPLGVTGPRLA
                     SLLALMRAEALEAGRDPAGLEVSLGHMVTKIDADRAGNLVDQGADRIVLAMPPTTDIE
                     QAKDLLSACAQRLSLPS"
     gene            11610..>11858
                     /locus_tag="VFI55_10750"
     CDS             11610..>11858
                     /locus_tag="VFI55_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7074543.1"
                     /translation="MTLSGDDRIALSDVVHRYAANVDDRQFDSVAALFTATADLVVPD
                     PPATLEPILTHRGRQAIAAAVATVAAVSRTEHAIIGEVY"
BASE COUNT         2023 a         4043 c         3749 g         2043 t
ORIGIN      
        1 ttataacccg aagccgccgt acccaactcc gccgccaact catcccacgc cgtcgcagca
       61 accatcatcg actcagaacc cggacccgca tacatccgac cggaattaat ctcgggcggc
      121 aacgccgcga agtcgaacac cgtgccctcc tcagttggcc gtgatcgcgg cggcaatctc
      181 ggttgtcatg taagaaccat ggtggccgcg gtcacgactt tgtgcatccg ctgggatcgc
      241 tgactcacgc ggaactctcg accagcaaac caggcgggca tggtggtccg taccaacttg
      301 cgcaccagcg gacagtgccc ggataggtgg tctgccacca ggattccgga atgtccggtc
      361 agaaacaaca tgcaccattt gcctcgttaa ctccgctgtc agcaaccgag cgacagctcg
      421 ttttcccctt gaactgccgc tcgcgaacga cattagctgg tctttccgga cacgcagacc
      481 ggggatgcca actgttcatt cccggccaac caatgttcac tcccggccaa ccacggttca
      541 ttgaagacaa ctaaatgttc accttcagtt cgcttattgc ttccggtcac cgggccggtc
      601 gagggccggg cgaatgcgtc ttacgcgcac gtcaagccgc tattccccgt cgatgatcag
      661 ggccatttcc ggacaggagg ccacaccgtc gcgaatcacc tgctcgtcct cgggtcggac
      721 ctcgtgctct tcgaggatcg agttgcccgc ctcgtcgatc gggaactgat ccggatcgac
      781 ggcgtaacac tgggcgtggc ccacgcattt ggacctttcg agacgaacct tcacaaggtt
      841 tccttcttcg tttccttctt cggcggtttc ggagaacacc cagaccggcc tggacaaagc
      901 cgcgacaaat ccggggcaaa tcggtggcta cagtagccgg tcgataaagt ctagaagacg
      961 gctgtcgatg cgggcggcca tgccgaatcg gccactaacc caccggattg gtgccccttt
     1021 gccgcacatg cgatattgat gtgccaccag aaacctgtga ctgcaaagtt ccaacaacgg
     1081 cctggctgcg tcggggaact aagacgccta agacacgaca acatacacag acaagcgaga
     1141 tctgagcgca tgagcagtgt tggcggaggc cagccgggca cgttctatct gccgcgactt
     1201 gaatactcca agttgccgtt gggcgccgac cgaggtgtcg gatggaagac attgcgtgac
     1261 gccgggcctg tggtgttcat gaacggccac tattacttga cgcgccgcga ggacgtgctg
     1321 gcggcgttgc gcaaccccaa ggtgttctcg tcgcgactgg cgcttcagcc ccccggtagc
     1381 ccgctgccgg tggtgccgct ggcgttcgac ccgccggagc acactcgtta ccgcaagatc
     1441 ctgcagccgt acttcagccc gcagggtttg agcgagtcca ggccggtgct gcagcgtcac
     1501 gccacggaga tgatcgccaa ctttgccccg cgtggcgaat gcgaggcgat ggcggatttc
     1561 gcgcggatat acccgtttca ggttttcctc gacctgtacg gcctgccgct ggaggacttg
     1621 gaccgcgtca tcgcctggaa agacgcgatc gtcgccgaca agccgtatat gaccagggaa
     1681 gacctggaca aggggcacga gctgctggcg tatctttccg acgcgatcca gcaacgccgg
     1741 caaaacccgg gctccgacat gttgtcgcga gtgatgtacg gggacggcga cttcagcgac
     1801 ctcgagttgc tgggtatgag ccacctgttg atcctggcgg gtctggacac ggtgaccgcg
     1861 gcgatcggtt tctccctatt cgaactggcg cgcagaccgg agctgcgtca cgcgctccgc
     1921 gataacccca agcagatcag ggttttcatc gaggagatcg tccgactcga accgtcggcg
     1981 ccggtggcgc cgcgggtgac gacggagatc gtcaccgtcg gcggcatgac gctgcccgcc
     2041 ggcacgcacg tgcggttgtg catggccgcc gtcaaccgcg acgacagcga cgcgatgtcc
     2101 accaacgacc tggtcatgga cggcaaggtg caccggcact ggggcttcgg cggcggacct
     2161 caccgctgcc tgggttcgca tctggcgcgg atcgaactga cgatcatcgt cgcggagtgg
     2221 ctcaagcaga tccccgactt cgaactgcct ccgggttact cccccgaaat caaatttccg
     2281 tcaaagacct tcgcgctcaa ggaattaccg ctgcactggg attgatcgcc gggttactcc
     2341 tgccaaaccc ggccaacccc agtgcagcgg cacagtccta actgagtcct ctccacacct
     2401 acttgcggaa ctcggtggct gccacctgga cgaacacacc ggtgtcctcg gccatcagca
     2461 ggcctcgacc acggggcagc gggccaccct tcatcttgcc gcggatgaag ccctcgtcgg
     2521 gatcggcgtc catcaccagc aacggcgcgt tcgcctggtg caacgaccgc agcatcgggt
     2581 cgctgcccgc cgaggaccag ccaccgaacg tgcgggtgac cagcacgtgc aagccgacgt
     2641 cggcggccct ggtcacccag gcggcggcct tgtgcagcgg tgaatcgaaa ccggccggta
     2701 gctgctggat gtcgtcgacg atcaagaaga tctccgggcc gctccaccac gaccgcgaca
     2761 acaactcttc cgcggtcagc ccgggcggag gctcacggcc ggccaggacc gcagccagct
     2821 cacccatcat cgcctgtacg ccgtcgaggt tgtaggcgaa tttctccaca tactcgcgac
     2881 cgagcacagt cagcagctga cggcgcgggt cgaccagcca cacctgggcg gagggccgcc
     2941 cgggaggggg cggcggtgcg ccggtggccc caggtgcata gagtcggccg atctcggaca
     3001 tgatggtggc cagcgtcgta gtgcgcccac attcgcgtcg ccccgtcacc atcaggtgcg
     3061 cgttctcggc gaagttgaga tagaccggct ccaggtccaa ttccgatatg gcccaggcga
     3121 ttccgcccac acctacccct tggcgggtgt cgcgggcggc gagctcgcgc acctgttgca
     3181 cgccgaaccg cgcgggtagc ctgcgcaccg ggcgcgcctg cccgctcgcg acgcggctga
     3241 ccgctgcgac gatgctgtcg gattcgaact cgttgttggg cgtaccgccc agcgccggcc
     3301 gggccaccaa cgtgtgcagg cccgcctgcg ggtcggcgtc gagtcggacg tagttgaccg
     3361 cgagcatgcc gcgtcccggc ttgaccggaa cttccttggc gaaccgggaa cgcaccagct
     3421 tggcatcttc caccgccgcc aatcgcaact cgacgcgaga cccgaagccg ctgcgcacgg
     3481 gtggccgcag ctcggattcg cgatcggcgc tgacgatcac gtgcaccccg aacgaggggc
     3541 cctggttgat gatcaggttc acctgctcga tcaggacctc gttctcctcg gccagcgccc
     3601 ggtagttgtc gatcaccagg tagacgtcgc cgaacacatc gggtacggga cccggtgcgc
     3661 cgccgaactt gcggcgccgg aacacctcca tcgaggcgat tccgtattcg aggaaggtgc
     3721 gcttgcggtc gcgtaccagc gccaacagct cggccaccgt ccggcgcacg ccgtacggat
     3781 ccgtgggacc gacgacctcg cccacatggg gcagaccggc gaccgtcgtc aacgcggtac
     3841 tgctgtatgc caggcagtag aactggacct gctcgggagt gtgggtcatc gccgccgagc
     3901 agatcagggt ctgcagcgcc gtggtcttgc ccgaaccgcc ggcgcccagg ataaggacgt
     3961 tggcgccagg cccggaggtg tcaaccgtcc acggcggctg gtcatgcttg aacgggcggt
     4021 cgatgatccc gatcgggaac accagatcct tggcggaacc gtagtccttc tgccacgggt
     4081 ggccgaggaa gcggttgacc aactcgtcga tggcgacggg ctgggtcagc ggcggttgcc
     4141 acaaccggta cggctcgaag tcaatcctgc gcagctgatg gatgatctcc atgccgacct
     4201 tcggtgtcct gatcgcgtcg tcctcgtcct cgacttgatc ggggtcctcc acctcgagca
     4261 cctcgccatt cgagtggacg gccaccggct cgatgtccgg tcccccgaca ctgacctcga
     4321 gcggagtgaa cgagttggta aacagttgcg ggcgaacgta gtcgatgctg tgcgccaaca
     4381 tcggcgcttc ctgcccgtcc atggtgatcc cgcgagcgta gaagtcccgc cacaggaact
     4441 cggcctggaa ccggacgatg tcctcaaggc tccggcggaa atatcccaga cccgcttggg
     4501 ccggcaggtt caccgcgttc ggcacaccgg cggcctgcgc cgccccggcg gtacgcgctt
     4561 tcaggaccag ccggtaaccc atgttctcca tgagcttttc ggcgcggctc tcaatggtct
     4621 gcgacgccat catcaggtgg atccagtagg cgcgaccctg ccggccgatc gagtccagca
     4681 cgtcgaccgc ggtcggcatg atgcggaacc attcgtagaa ctcgtcgatg accaccacga
     4741 gcatcggcag cggcggcatg tcctggccgc gggcgcgcat tctggaccgc acggagttgt
     4801 attccttggc atcgtcgacg cctgcgttgt cgcagaccgc cttgcggcgc gcgatctcac
     4861 cccagagtgc gtccaggaag cgctccatca gcgcctggtc ttcctcgagg tcggtgatga
     4921 tccgcgagac gtgcggcact ccggagaagg gcttgacggc tgacccaccc ttgaggtcgg
     4981 ccaggacgaa ctgcagctcc tccggcgggt gaccgagcat cagcgattcg atcacggttc
     5041 gcaccagcgt cgacttaccg gaacccgtcg ttccggacat gacaccgtgc ggaccgtcgc
     5101 cgccttcgtc cagggacttc atgtccaaga acagcaactc gccgttgtct gagcgattac
     5161 cgaacggtgc gcgcaaccgc gaccgtccca tggtgtccgt gcggctgccc cacaacgcgt
     5221 cgaagtcgat atcggaagga tcgtcaatcc cgtagtagga caagatgtct cgggctccga
     5281 ggtgggccac ccgctggccg atctcctcgt aggcctcggc cagccgccag cgcgcgagct
     5341 tctgagcgaa ctcctccgct tccacggcgc tcagatggtc ggtgagggcg aagaaccaag
     5401 gcttctcgtc gatgaccatc caggtgtcgc gatcgcgggg cagcgcctcg atcacgcccc
     5461 tttcgtcgaa ccgcaacgtc cgctccccga ccgtcgacca catcgaaccg ccggtcaggt
     5521 cgaagaacgt cacgccgtcg ataccttcgg cgctgatcac gtactcccat tgcggatccg
     5581 tgacgtcggc gatgatcacg gtgtgcggcg tcgggcactg cgccgatgaa ctggcatggc
     5641 gcggcgtgaa agacccacgg ccggcgaaca attcggcttg ctccgcggcg aactcccgca
     5701 ccgagctgta gaccatccgc gcattgcccg ccgcgtcctg gcgccgctgg tcaccgaagt
     5761 gcggaagcca cttcacccag tcccattgct ccaaatccga actgaccacg atcaactgca
     5821 tgtggtcggg tccgtgcgag aacgtcagct ggcagacgat cgcgcgcatc agtcccagca
     5881 cctgctcgcg gtctccgatc agcgagtacc agggttcaac caggacggaa atcatcttgg
     5941 gcaggttgta gacgacgctt tgatagcgac cgaactcctg aagtgcctta cctgtcaccg
     6001 gctccagctc gatgtcggtc ggcatgtttt gaggctcacc ccaggtcacc tcgggacgcg
     6061 tcatgcccac gccgactcgg actacaccaa agttgaggtc cttaccgtcg ggcttgcgtt
     6121 cccacatccg cgacgatccc accgcggccg gcagcgtgtc gggtgacggg tggaaccacc
     6181 ggtagttggc gtccatgctg tcggccgact cgtgggcggt ttcacgcagc atgtcgagca
     6241 tcagcatgaa ctgggcgcgc atcgcgtcca gcttcggccg actcatctgc tgctggccac
     6301 cacccatgcg gccgccgaac atcatcatcg cgacgccgcc gatcatgaag atcgggaaga
     6361 tcgagccggc gcctccgaac acgtgtgagc cgctggcgaa ggtcatcgcg accatgccga
     6421 tcagcaggcc gacgaccaac accccgacca cgatcagcca ccagggcttg ccctccggcg
     6481 gagggatgct caacggcgtc ggcaggacaa tgttctccgg cttgatcacc ggcgcctttt
     6541 ccggcgtcgg tcgtgcaaat ccgcgtttca cttgggtacc accaactctg caggggacat
     6601 gtccatgggt agggtgtcgt gctcgaccag ggcgtctgag cgtgacaggg tcggaccctg
     6661 tggcaacagc cgcagcgcca cccacggcgc caggctcggg gccgtcttca gacccaatgc
     6721 ctcgcgcacc tcgcgggtgt cgtccactcc gaaccgagcg ccggcatcgg tcagccacca
     6781 gagcgattcc gttgtcttgg cgccgggatc attgccggtg acagccacga agttcgcgta
     6841 gtcggggccg aagaagacct ggtcggcctg ccggccagtc gtgtcggcct tcaccaacga
     6901 caccaccttg gccatgtcct tctgcgtgac cgggatggtc gacccggaca cgacttgcac
     6961 acgggcccgg ttctcaccgg aagtcttctg ccaccaccag caggtcgccg ggttctcccg
     7021 aatatccatc acgttcaacg ggttgtccgg ataggacgac aagtccagct tgttgaccac
     7081 cggcattttc gccaatgcgg ccggctccac ggtcaccggt ttggtgttgc ccgggccgcc
     7141 ggcgttctgc aggatctggg ccaccaacgg tggcagcgtc tgcacaccat cggtcaacac
     7201 cagagaatat tgttgcggcc cgctgatttg tggtgtcacg agcaccgtgc ccaccgcgcc
     7261 cggcgccccc gagaatgccg ccgcagcgcc cgcgttcggc acgtccggca ccgtcaactc
     7321 gggtccgacg ggcaacgcgt cgaacagggc gcggctcatc ggcttcgcca tctcgacttc
     7381 ttccggggtc aagcccagcg gcaacaacac cgaccggttc gtcgcatcga cgcgcgaccg
     7441 gcgtccctgc cggatgaccc aggcgtcccc gccgtagctc agcaccactg catccgaccc
     7501 gttcaggact tggcggtggc cgctgaggtc cggggtgccg tcgatcacgg tcaccgtcac
     7561 ccctgaggga gccccgaggc ccatcgaggt gcccaccgtg tcgcagacca gccacgacga
     7621 cgcgggcgga gtcttggtcg caaagttgga cggcgcaccg gggatgccca ccagcggccc
     7681 ccgcggcagg ccggcgattt ggcttccccg aacgagatgc gggttgtccg gccgtccggt
     7741 gatcagccgc gccgatgcca ggttcagcgc cgggtacagc ctgtcaccca cgcggacgta
     7801 cagcgcaccg gagtcgcggt cggcgatgat aggcgactcg ttgatctggc cggacgggct
     7861 gatgaacgaa tacagcagcg cacccaggca gatgaccaac gcagcggaaa ccgaagccac
     7921 cacggccagc gtctggcgcc gtcccggctc gacctccatg cggacccgcc accgggtcag
     7981 tgccatcgcg gttcggcgag cgaggaactg atagccggtt acctgggtcc gcgtcgacaa
     8041 gcccaggccg taccccgacc ctcgccctcc gcgactctct tccgccacgt taattcacca
     8101 tccggtctgc tagatcgccc tggacattgt ccaacgcgcg aattacatcg acaaccgtaa
     8161 gtgacccgca cggagctcgc cacgcttcgc cgtgcgcgtt cacttttatt cacgtcttgt
     8221 tgttcagtcg aatccgccct ttatcggctc gccaggcctg cgaccggtgc cagcggcggc
     8281 acacgccgct gagctggcac ttttggtttt ccccactaat cccccggccg gattcttttg
     8341 tgtcttcatt gggcatggta gccgcggacg gccaactcgt cctgctaaac gggtataccg
     8401 ccactggctt cctggcctgc atagtaatgg ttcgccagcg caggtctcag caaaatcaac
     8461 cactggccgc accgcagccg cctcgctagc cgcgcccgac aggctcccag gggtcgcgtc
     8521 aagcaagatg ggcggcatga ctgagctcgc gccctcgctg gtcgaactgg cccggcggtt
     8581 cggcatcgcg accgagtatg acgactggac cggccggcga gtgctcgtct cggagatcac
     8641 gctggtggcg gtgctcgcag cgctcggtgt cgccgggggc accgagcaag agcgcaatgc
     8701 cgcactgacc gcgcaactgc ggtcgtactg ggcacgtcgg atgccgccga cgatcgtcgg
     8761 gcgcaccggc gtccagacgc gtttctgggt gcatgtgacg cacggccatc ccgccgaggt
     8821 ctggttgcag ctcgaggacg ggaccatgcg cggcgggttg cagcaggtcg acaacttcac
     8881 accgccattc gatctggatg gacgctgggt cggggaggcc accttcgtgg tgcccgccga
     8941 cctgccgctg ggctaccacc gggtgcatct gcgttccggc gacgccgaga cgagcaccgc
     9001 gctcatcgtg acgcccgact ggctcgggct gccggagcag ctcggcgccc gccgcgcgtg
     9061 gggtctggcc gttcagctct acagtgtgcg gtcccgacag tcctggggta tcggcgatct
     9121 caccgacctg accgacctcg cggtctggtc ggcgtcccgc cacggcgccg attacctcct
     9181 ggtcaatcct ttacacgcgg ccggggtttc cgggccgacc aaccgcatgg agccgtcacc
     9241 gtatctgccg acctcgcggc gcttcgtcaa cccgctgtac ctgcgcgtcg aggcggtccc
     9301 cgaattcgcc gaactgacca agcggagccg ggtgcggcgg ctgcgcgcgg acgtccagcg
     9361 ggacgccgcc aaactcgacg ccatcgaccg cgatagcgcc tgggcagcca agcgggcagc
     9421 gctcaagctg ctgcatgcag aaccccgatc ggccggccgc gagctggctt actcagcatt
     9481 ccgcgaccgt gagggccgtg cgctcgacga cttcgcctcc tggtgcgcgc tggcagaaga
     9541 gtacggcggt gactggcatc gctggccgga gtcgctgcag catcccgacg ctgccggtgt
     9601 cgccggtttc gtcgaaagga attcggacac aatcgatttc caccgctggc tgcagtggca
     9661 actcgacgag cagctcgcct cggcgcagtc gcaggcaata cgcgcgggca tggcgttggg
     9721 catcatggca gacctggccg tcggtgtgca tcccaacggc gccgacgcct gggccctgca
     9781 ggatgtgatg gcgcggggcg tgaaggccgg tgcgccaccc gacgagttca atcagctcgg
     9841 ccaagactgg tcacagcccc cgtggcgacc ggaccggctg gatgagcagg agtatcgccc
     9901 gtttcgtgcg ctgatccagg ccgtcctgcg gcatgccggg ggcgtccgca tcgatcacat
     9961 catcggactg ttccggctgt ggtggattcc cgacggcgca ccgcccaccc aaggcaccta
    10021 cgtgcgctac gaccacgaag cgatgatcgg catcgtcgcc cttgaagcac atcgggcggg
    10081 ggcgctggtc gtcggcgaag atctgggcac ggtcgaacca tgggtgcgcg actacctttt
    10141 attacggggc gtgctgggca cctcgatcct gtggttcgag ctggatcgcg acggaaccgg
    10201 cgggccgttg ccggcctcgc gctggcggga gtactgcctg tcgtcggtca ccacccatga
    10261 cctaccgccg actgccggat atctggtcgg cgaccatgtc ctgctgcggg agtcgctggg
    10321 gttgctgacc cggcccgtcg aaatcgagct cgagtccgac cgcgccgagc tggccggctg
    10381 gatggccgag ctgcgccggg tcggcttgct cgacgacggt gaagacgatc cggatcagat
    10441 cgttctcgcg ctgtatcggt atctgggccg gacgccgtcg cggctgctcg gggtggccct
    10501 gaccgacgcg gtcggtgacc accggaccca gaatcagccg ggcaccaccg acgaataccc
    10561 caactggcgg gtgccgctga ccggccccga cggccgccag gtgatgctcg aagacgtgtt
    10621 caccgatcgc cgggccgcca ccctcgccga ggcggtgcgc accgagatcg cgccccgggg
    10681 ttgagggcta cctgttagct tcgggtccca tgaaggtttg cgtcgtggcc ccggtcgccg
    10741 acggtgtcac cgcggacccc gattggatgg tgagcttcgc gcgtcatctc gaacggtgcg
    10801 ggttcgaatc gatcgtcgtc gtcgagcaca ccgtcctggt cacccggtac gacagtgttt
    10861 atccgtacga cagttccgga cgagttggcc tggcggccga ctgctctatc ccagacccgc
    10921 tcgatctgct cgcctttctg gccggccaca ccacccggct cgggctggcc accggggtgc
    10981 tggtactgcc caaccaccat ccggtggtgc tcgcgaaacg ggcggcgacc gtcgatgcgc
    11041 tctccggcgg acgactgcgg ctgtgcgtgg gggtgggctg gctccaggaa gaggtggagg
    11101 cgtgcggcgc ggaattcgac agccggggcc gccgcgccga cgagcagttg gcagtgttgc
    11161 ggacactgtg ggccgaccag ccgcgggggg cctcgtatca cggtgagttc ttcggcttcg
    11221 acgacgttat gtgctacccc aaaccagttg cgggcgaacg ccttccgatt cacatcggtg
    11281 ggcacagccg ggccgccgcc cgccgcgccg gacgcttcgg cgacggcttc cagccgctcg
    11341 gtgtgaccgg tccccgcctc gcctcactgc tcgcgctgat gcgtgcggaa gcgttggagg
    11401 cgggccgaga cccggcgggc ctggaagtat cactgggcca tatggtcacc aagatcgacg
    11461 ccgaccgcgc gggcaacctg gtcgatcagg gcgccgaccg gattgtgctg gccatgccac
    11521 cgaccaccga catcgagcag gccaaagacc tgctgtccgc atgcgcgcaa cggctctccc
    11581 tgccgtcatg aacccggcct aattctgcga tgacactctc aggcgacgac cggatcgccc
    11641 taagcgacgt ggtgcaccgc tacgcggcga acgtcgacga ccgtcaattt gactctgtgg
    11701 cagcgctttt cactgcgacc gccgatctgg tggtacccga tccgccggct acgctggagc
    11761 cgatcctcac ccatcgtggc cggcaggcca tcgccgccgc cgttgccacc gtcgccgcgg
    11821 tgagccgcac ggagcacgcg atcatcggtg aggtctac
//
LOCUS       DASSGQ010000244         4398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_503507,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000244 DASSGQ010000000
VERSION     DASSGQ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503507"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1736)
                     /locus_tag="VFI55_10755"
     CDS             complement(111..1736)
                     /locus_tag="VFI55_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7074544.1"
                     /translation="MLVAATTLTSTLTGCSGSTAPQVDYVVDGQLVTYNTNTVVGAAS
                     AGAQAFARTLTGFGYHGPDGQIVADHDFGTVSVVGGAPLVLDYQIADNAVYSDGKPVT
                     CDDLVLAWAAQSGRFPGFNAATQAGYLDIANVECIPGQKKARVSFIPDRSVVDYTQLF
                     AATSMMPSHVIEDELNVDVTAALLNNNAQVVDRIAKVWNNGWDLKPGLKPDEALKRFP
                     SSGPYRIESILDGGAVVLVANDRWWGPKPITKRITVWPQGPDIQDRVNNRSVDVVDVA
                     AGSSGTLATPDNYDRTDSPSGGIEQLIFAPQGPLAQPKARRAFALCTPRDTIAHDAGV
                     AIANSRLQPATDDAIAAGEGAAEAGQFSKADPAAARDALGGAPQTVRIGYQGPNARLA
                     VTVGVITKSCAPAGITVANVTLDSSGPQALRDGKIDVLLASTGGATGSGSTGSSAMDA
                     YDLHSGNGNNLSGYANPQVDSAIGALVVSADPAERARLLVEAAPPLWADMPTLPLYRQ
                     QRTLLISKKMYAVTRNPTRWGAGWNMDRWALMQ"
     gene            complement(1802..3112)
                     /gene="secF"
                     /locus_tag="VFI55_10760"
     CDS             complement(1802..3112)
                     /gene="secF"
                     /locus_tag="VFI55_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HET7074545.1"
                     /translation="MSASKASRDATETDAAAVEVADSAAQLPHHSFLSRLYTGTGAFD
                     VIGHRRLWYGISGAMVAIAIISILVRGFTFGIDFKGGTTVSMPAASGTGTVSTTQVSD
                     VFAKALGSAPASVVVVGSGASATVQISSETLSNDQTTKLRNALFDAFAPKGTDGKPSK
                     SAISDSAVSETWGDQITKKALIALVVFLALVSLYITVRYERYMAISALTTMCFDLTVT
                     AGVYSLVGFEVSPATVIGLLTILGFSLYDTVIVFDKVEENTHGFQHTTRRTFAEQANL
                     AINQTFMRSINTTVIGVLPVLALMVVAVWLLGVGTLKDLALVQLVGILVGTYSSIFFA
                     TPLLVTLRERTQLVRTHTRRVVKRRSPGARAGAEADPRDDETSSDKTSDEVSDETQAE
                     TAEHATTAAPASGKPAPNKPAPGARPVRPTGTRRPTGKRNAGRR"
     gene            complement(3116..>4398)
                     /gene="secD"
                     /locus_tag="VFI55_10765"
     CDS             complement(3116..>4398)
                     /gene="secD"
                     /locus_tag="VFI55_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET7074546.1"
                     /translation="PGAQPRPYPEDPPPTPSPAPPAPPAPGAPPPAPGAPAGQAPPAE
                     APAPADPRKDLADRIADEKKWRQSTRQGIQYLALQFQATRCDKDDILAGNDDPTLPLV
                     TCSTDHKVAYLLGPSIISGDQIENATSGMNQRGLGYVVDVQFKPAAANIWADFTAAHV
                     GTQTAFALDSQVVSAPVIQEAIPGGRTQITGGDPPFTASTARQLANVLKYGSLPLSFE
                     SSEAQTVSATLGLTSLRAGLVAGAIGLVLVLVYSLLYYRVLGLLTALSLTCSGAMVFA
                     ILVLLGRYIDYTLDLAGIAGLIIGIGTTADSFVVFFERIKDEIREGRSFRSAVPRGWV
                     RARKTIVSGNAVTFLAAAVLYFLAIGQVRGFAFTLGLTTILDLVVVFLVTWPLVYLAS
                     KSPTLAKPAYNGLGAVQQVARERRASANVKTGRG"
BASE COUNT          687 a         1444 c         1550 g          717 t
ORIGIN      
        1 ctggacgccc ggctccggaa aatacgccac ttcccgcgtc agcgtcgcga tcagtgcggc
       61 cacccgcggc gcgctcgtca ccggagacgc accggagtct gagtggaagc tcattgcatc
      121 agggcccatc ggtccatgtt ccaacccgcg ccccagcggg tcggattcct ggtgacggca
      181 tacatcttct tcgatatcag cagcgtgcgc tgctgtcggt agaggggcag cgtcggcata
      241 tcggcccaca gtggcggagc cgcctccacc agcagccggg cccgctcggc ggggtcggcc
      301 gacaccacca gcgcaccgat cgcgctgtcg acctggggat tcgcgtagcc ggacaggttg
      361 ttgccgttgc cgctgtgcag gtcgtaggcg tccatggccg acgatccggt cgatccgctg
      421 ccggtggcgc cgccggtgct ggccagcaac acgtcgatct ttccgtccct gagcgcctgg
      481 ggccccgagg agtccagggt gacgttggcg acggtgatac cggccggggc gcaggatttg
      541 gtgatcaccc cgacggtcac cgccagccgc gcgttgggcc cctggtaacc gatccgcacc
      601 gtctgggggg cgccacccag cgcatcgcgg gcggcggcgg gatcggcctt gctgaactgg
      661 ccggcctcgg cggcaccctc accggcggcg atggcgtcgt cggtggccgg ctgcaggcgt
      721 gaattggcga tcgctacccc ggcgtcgtgc gcgatcgtgt cgcgcggagt gcacagggcg
      781 aacgcacgcc gcgccttggg ctgcgccagc ggtccctgcg gggcgaagat cagctgctcg
      841 atgccgcccg acggcgaatc ggtgcggtcg tagttgtccg gggtggccaa cgttccggac
      901 gagccggccg cgacgtcgac cacgtcgaca ctgcggttgt tgacccggtc ctggatatcg
      961 ggaccctgcg gccacacggt gatccgcttg gtgatcggtt tgggacccca ccagcgatcg
     1021 ttcgcgacga gcaccaccgc gccgccgtcg aggatcgatt cgattcggta cggtcccgaa
     1081 gacgggaagc gcttcagggc ttcgtcgggt ttgagaccgg gcttgagatc ccagccgttg
     1141 ttccacacct tcgcgatccg gtccaccacc tgcgcgttgt tgttcagcag cgccgcggtc
     1201 acgtccacgt tcagctcgtc ttcgatgacg tgcgacggca tcatcgaggt cgcggcgaac
     1261 agctgcgtgt agtcgacgac gctgcgatcc gggatgaacg acacccgggc cttcttctgg
     1321 cccgggatgc actcgacgtt ggcgatgtcg aggtagccgg cctgggtggc ggcgttgaag
     1381 cccgggaaac ggccggattg ggcggcccag gcgagcacga ggtcatcgca ggtcaccggc
     1441 ttgccatcgc tgtagacggc gttgtccgcg atctgatagt ccagcaccaa cggggcaccg
     1501 ccgacaaccg agacggtgcc gaagtcatgg tcggcgacga tctgtccgtc gggaccgtga
     1561 tagccgaagc cggtgagcgt gcgggcaaaa gcctgagctc cggccgacgc ggccccgacg
     1621 acggtgttgg tgttgtacgt gaccagttgg ccgtcgacca cgtagtcgac ctgcggggcg
     1681 gtgctgccgg agcagccggt gagggtggaa gtcagcgtgg tggccgcgac gagcattccc
     1741 gccagcccgg ccggaccaat ggatgccgat cccttcggcc ctcgccaacc gggcatagtg
     1801 cctaccgccg gccggcgttt cgcttgccgg tcggacgcct ggtcccggtg ggccgcaccg
     1861 gacgcgcgcc cggcgccggc ttgttcggcg cgggcttgcc cgacgccggc gccgcggtgg
     1921 tggcgtgctc cgcggtttcg gcttgggtct cgtcagaaac ctcgtcggaa gtcttgtcgg
     1981 agctagtctc gtcgtcccgg gggtcggcct ctgcacccgc tcgcgcgccg gggctgcgcc
     2041 gcttgacgac ccggcgggtg tgcgtgcgca ccagctgcgt gcgttcgcgc agggtgacca
     2101 gcaacggggt ggcgaagaag atcgaggagt aggtgccgac caggatgccg accagctgca
     2161 ccagcgccag gtccttcagc gtgccgacgc ccagcagcca caccgccacg accatcagcg
     2221 ccagcaccgg cagcacgccg atcacggtgg tgttgatgga gcgcatgaac gtctggttga
     2281 tcgccaggtt ggcctgttcg gcgaaggtgc gccgggtggt gtgctggaag ccgtgggtgt
     2341 tctcctcgac cttgtcgaac acgatgacgg tgtcgtatag cgagaaaccc aggatggtca
     2401 gcagaccgat caccgtggcg gggctgacct cgaagccgac cagtgagtac acgcctgcgg
     2461 tgacggtcag gtcgaaacac atggtcgtca gcgcggagat ggccatatag cgctcgtaac
     2521 gcaccgtgat gtagaggctg accagcgcca ggaacaccac cagcgcgatc agggccttct
     2581 tggtgatctg atcgccccag gtctccgata ccgccgagtc actgatcgcc gacttgctgg
     2641 gcttaccgtc ggtgcccttg ggcgcaaagg cgtcgaacaa cgcgttgcgc agcttcgtcg
     2701 tctggtcgtt ggacagcgtc tccgaactga tctgcaccgt cgccgaggcg ccgctaccca
     2761 cgaccaccac ggatgctggg gcgctgccca gcgccttggc gaacacgtcg gaaacctgcg
     2821 tggtcgacac tgtgcccgtc ccgctcgcgg cgggcatcga caccgtggtg ccgcccttga
     2881 aatcgatccc gaaagtgaag ccccgcacga ggatgctgat aatggcgatc gccaccatcg
     2941 cgccgctgat gccgtaccac agccgacggt gtccgatcac gtcgaacgcg ccggtccccg
     3001 tgtaaagccg agacaggaag ctgtggtgcg gcagctgggc ggcgctgtcg gccacctcga
     3061 cggcagcggc gtcggtctcg gtcgcgtctc tggaggcctt ggaggcactc atgcgctatc
     3121 cccgtcccgt cttcacgttt gccgaagccc ggcgttcgcg cgcgacctgc tgaacagctc
     3181 caaggccgtt gtacgcgggc tttgccagcg tcggtgactt ggaggccagg tacaccagcg
     3241 gccaggtcac caagaacacc accaccaggt cgaggatggt ggtcagaccc agcgtgaagg
     3301 cgaatcccct gacctggccg atcgccagga agtacagcac cgcggcggcc aggaaggtga
     3361 cggcgttgcc cgacacgatc gtcttgcggg cccgcaccca cccccgcggc accgccgaac
     3421 ggaacgaacg gccttcgcgg atttcgtctt tgatgcgttc gaagaacacc acgaacgagt
     3481 cggcggtggt tccgatgccg atgatcagac ccgcgatgcc cgccaggtcc agcgtgtagt
     3541 cgatatatcg gccaaggagc accaggatcg cgaaaaccat tgcgccggag caggtcagcg
     3601 acaacgccgt gagcaacccc agcacccggt aatacagcaa cgagtacacc agcaccagca
     3661 ccaggccgat cgcgcccgcg accaggccgg cccgcagcga ggtcaatccc aatgtcgcgg
     3721 aaacggtctg ggcttccgag gattcgaagg acagcggcag cgacccgtat ttgaggacgt
     3781 tggccaactg ccgcgcggtg gaagcggtga acggcggatc gccaccggtg atctgggtgc
     3841 ggccgccggg gatggcttcc tggatcaccg gggcgctgac cacctgcgag tccagcgcga
     3901 aggcggtctg ggtcccgaca tgggcggcgg tgaagtctgc ccagatattc gccgccgccg
     3961 gcttgaactg cacgtcgacg acgtagccga ggccgcgctg gttcatgccc gacgtggcgt
     4021 tttcgatctg gtcgccgctg atgatcgagg ggcccagcag gtaggccacc ttgtggtcgg
     4081 tcgagcaggt gaccaggggc agcgtcgggt cgtcgttgcc ggccaggatg tcgtctttgt
     4141 cgcagcgggt cgcctggaac tgcaaggcga ggtactgaat gccctggcga gtgctctgcc
     4201 gccacttctt ctcatcggcg atgcggtcgg cgagatcctt gcgcgggtcg gccggcgcgg
     4261 gcgcctcggc cggcggcgcc tgtcccgcgg gcgccccggg tgccgggggc ggagcgcctg
     4321 gcgcgggagg cgcgggcggc gcggggctgg gtgtcggcgg cgggtcctcc gggtaaggcc
     4381 ggggctgggc gccgggtc
//
LOCUS       DASSGQ010000245         5418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1444872,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000245 DASSGQ010000000
VERSION     DASSGQ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444872"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1439
                     /locus_tag="VFI55_10770"
     CDS             150..1439
                     /locus_tag="VFI55_10770"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7074547.1"
                     /translation="MDSDGTIGDVTTHQLPTHTAGHHRQRAFAQSSKLQDVLYEIRGP
                     VHAHAARLEAEGHRILKLNIGNPAPFGFEAPDVIMRDMIQALPYAQGYSDSQGILPAR
                     RAVVTRYELVEGFPRFDVDDVYLGNGVSELITMTLQALLDNGDQVLIPSPDYPLWTAS
                     TSLAGGTPVHYLCDETQNWQPDIADLESKITERTKALVVINPNNPTGAVYSREVLSQM
                     VGLARKHQLLLLADEIYDKILYDDAKHISLASLAPDMLCLTFNGLSKAYRVAGYRAGW
                     LAITGPKDHAGSFIEGISLLANMRLCPNVPAQHAIQVALGGHQSIEDLVLPGGRLLEQ
                     RDVAWTKLNEIPGVSCVKPEGALYVFPRLDPEVYDIADDEQLVLDLLLQEKILVTQGT
                     GFNWPAPDHLRIVTLPWSRDLARAIERLGNFLASYRQ"
     gene            1471..2745
                     /locus_tag="VFI55_10775"
     CDS             1471..2745
                     /locus_tag="VFI55_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HET7074548.1"
                     /translation="MAHSHSHSLRSSSEAPSGLDPLPAKIVIGLLIAIGVAVIAGAVV
                     LWPSRQHVDIPMPFQNASGGAVSTQRGHVLSSALGDCGSPSSGRVLTTTPLPGTPGAG
                     RCVETLVAIDSGPNAGATTLLESSPGPGQPQFAAGDHVRVARQVDAQGTTTYAFYDFE
                     RGWPLVALGIIFAVVIVAVARWRGLLAMVGIVVAFLVLVVFLLPALRDGAPAIPVALV
                     ASAAILYAVIYLAHGVNLRTSAALLGTLSSLLLAAGLSWAAVDLAHLTGLSDDQNSQV
                     AAYLGNVSISGLLLAGFIIGSLGVLNDVTVTQASTVFELAHLGGSRRAIFLGAIRVGR
                     DHIASTVYTLVLAYAGTSLPLLLLFSVANRSLGDVLISESVAIELARSAVGGIALALS
                     VPLTTAIAAVLAKPAGISSAPTTPADSRRTRT"
     gene            complement(2696..3562)
                     /gene="rfbA"
                     /locus_tag="VFI55_10780"
     CDS             complement(2696..3562)
                     /gene="rfbA"
                     /locus_tag="VFI55_10780"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET7074549.1"
                     /translation="MRGIILAGGSGTRLHPITIGISKQLLPVYDKPMVYYPLSTLMMA
                     GIRDILVITTPHDAAGFQRLLGDGSQFGINLTYVMQEQPDGLAQAFVLGANHIGTESV
                     ALVLGDNIFYGPGLGTSLSRFQNLRGGAIFAYWVANPSAYGVVEFGADGMALSLEEKP
                     ETPKSNYAVPGLYFYDNDVIEIAKGLQKSPRGEYEITEVNQIYLNRGRLGVEVMARGT
                     AWLDTGTFDSLLDASDFVRTLERRQGLKVSVPEEVAWRQGWIADEQLAAWARTLVKSG
                     YGDYLLELLERS"
     gene            complement(3579..3947)
                     /locus_tag="VFI55_10785"
     CDS             complement(3579..3947)
                     /locus_tag="VFI55_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7074550.1"
                     /translation="MTTSEIATVLAWHDALNASDLATLVALSSDDIDIGDSHGAAQGH
                     QALRTWAASQEGTAEPGRMYVHHGVVVVEQKVTAPDGTVSDAASAFRVVHDHVTSVFR
                     HHDLASALAATELTEADLVS"
     gene            complement(3976..4731)
                     /locus_tag="VFI55_10790"
     CDS             complement(3976..4731)
                     /locus_tag="VFI55_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HET7074551.1"
                     /translation="MDYAGAFLDENRAFAELFRDVDGSTPVPTCPGWSLTQLLRHVGR
                     GDRWAAQIVRDRLDQALDFRAVEGGKPPPDPADAISWLYGGAQRLVDAVELSGAETPV
                     WTFLGPRPANWWIRRRLHETAVHRADAAIATGTDFTLDSGIAADGISEFLERVAIQAG
                     SAGTPLPLEEGNTVHLHAGDPGLGEAGEWTIGVEGGEITWSHEHGKGSVALRGGATEL
                     LLAMTRRVSLADTGIELFGDAAVWQNWLDRTPL"
     gene            4871..>5418
                     /locus_tag="VFI55_10795"
     CDS             4871..>5418
                     /locus_tag="VFI55_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015345094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HET7074552.1"
                     /translation="MSFVNAAPGALASAASDLAGIGTTLSSASAAAAAPTTGVVASAA
                     DEVSAAVASLFSGYAQDFQELSAQTTAFHDQFVQTLSAGAGSYASAEAANVQQTLLGA
                     INAPSEALVGRPLIGNGANGAPGTGQNGGNAGILIGNGGAGGSGAPGQNGGNGGAGGL
                     LGAAGAGGAGGASTTGPGGNGG"
BASE COUNT          849 a         1848 c         1822 g          899 t
ORIGIN      
        1 cccgaaccgc agccgtcggc ccaagccggc ggcgacccgg caccgccggc agcacccgtg
       61 aaaggactgg gaatcgcacg cggtgcccgc cctccgggca agcgctgatc gaaggtttgg
      121 cagttcaagc ccgtagtcag caaatttcat tggacagcga tggcaccata ggtgacgtga
      181 ctactcacca gctgcccacg cataccgccg gccatcaccg gcagcgggcc ttcgcgcagt
      241 cgtccaaact ccaggacgtt ctgtacgaaa tccgcggccc ggtacacgcc cacgctgcgc
      301 ggctggaagc cgaaggccac cgcatcctca agctcaacat cggcaacccg gcgccgttcg
      361 gcttcgaggc gcccgacgtg atcatgcgcg acatgatcca ggcgctgccg tacgcacagg
      421 ggtactccga ctcgcagggc atcctgcccg cccggcgcgc ggtggtcacc cgctatgagt
      481 tggtggaggg ttttccccgc ttcgacgtcg acgatgtcta cctgggcaat ggcgtctccg
      541 agctgatcac gatgacgctg caggccctgc tcgacaacgg agatcaggtg ctgatcccgt
      601 cgccggacta cccgctgtgg acggcgtcga cctccctggc gggcggcaca ccggtgcact
      661 acctgtgcga cgagacccag aactggcagc cggacatcgc cgacctggag tccaagatca
      721 ccgagcgcac caaggcgctg gtggtgatca acccgaacaa cccgacgggc gccgtctaca
      781 gccgggaagt cctgagccag atggtcggtc tggcccgcaa gcatcagctg ctgctactgg
      841 ccgacgaaat ctacgacaag atcctctacg acgacgccaa acacatcagc ctggcgtcgc
      901 tggcgccgga catgctgtgc ctgaccttca acggcctgtc gaaggcctac cgcgtcgccg
      961 ggtaccgggc cggctggctg gccatcaccg ggcccaagga tcacgccggc agcttcatcg
     1021 aaggcatcag tctgctggcc aacatgcggt tgtgccccaa cgtcccggcg cagcatgcga
     1081 tccaggtcgc cctcggcggc caccagagca tcgaagactt ggtgcttccc ggcggtcggc
     1141 tactggaaca acgcgacgtc gcctggacca agctcaacga gattcccggg gtgtcctgcg
     1201 tcaagccgga gggcgcgctg tatgtgttcc ccaggctgga tcccgaggtc tacgacatcg
     1261 ccgacgacga gcaattggtc ctcgacctcc tgctgcagga gaagatcctg gtcacccagg
     1321 gaaccgggtt caactggccg gcaccggatc acctgcgcat cgtgacgctg ccgtggtcgc
     1381 gtgacctcgc cagggcgatc gagcgcctgg gcaacttcct cgccagctac cggcagtaag
     1441 cgggtttcgg cgccctctac cctcgaccgg gtggctcact cacactcgca cagcttgcgc
     1501 agctcgtcgg aggccccctc cgggctggat ccactgcccg ccaagatcgt catcgggctg
     1561 ctgatcgcca tcggtgtggc cgtgatcgcc ggtgcggtcg tgctctggcc gagccggcag
     1621 cacgtcgaca tcccgatgcc gttccagaac gcctccggtg gcgccgtgag cactcaacgg
     1681 ggccacgtgc tgtccagcgc gctcggcgac tgcggcagcc cgtcgtcggg tcgggtgctc
     1741 accaccacgc cgctgccggg aacaccgggc gccggacggt gcgtcgagac gctcgtcgcg
     1801 atcgattccg gaccgaacgc cggcgccacc acgttgttgg agtcctcccc cggccccggc
     1861 cagccccaat ttgcggccgg cgatcacgtt cgggtggcgc ggcaggtcga cgcgcaaggc
     1921 accaccacgt acgcgttcta cgacttcgaa cgcggctggc cgctcgtcgc actcgggatc
     1981 atcttcgcgg tggtgatcgt cgcggtggcc cgctggcgcg ggttgctcgc gatggtcggc
     2041 atcgtcgtcg cgttcctggt gctggtggtg ttcctgctgc cggcgttgcg tgacggcgca
     2101 ccggcgatcc cggtggccct ggtggcgtcg gcggcaatcc tctacgccgt gatctacctg
     2161 gcccacgggg tcaacctgcg caccagcgcc gccctgctgg gcaccttgtc gtcgttgctg
     2221 ctggcagcgg gattgtcgtg ggccgcggtc gatctcgccc atctgaccgg gctgtcggac
     2281 gatcagaatt cgcaggtcgc cgcgtatctg ggcaacgtat cgatcagcgg cctgctgctt
     2341 gccgggttca tcatcgggtc gctgggtgtg ctcaacgacg tgaccgtgac ccaggcgtcg
     2401 acggtgttcg agctcgccca tctcggcggc tcccggcggg ccatcttcct gggcgcgatc
     2461 cgggtgggcc gcgaccacat cgccagcacg gtgtacacgc tggtgctggc ctatgccggc
     2521 acgtcgctgc cgttgctgct gctgttcagc gtcgccaacc gatcgctggg tgacgtgctg
     2581 atcagcgaga gtgtggccat cgagctggcc cgctccgcgg tcggcggcat cgcgttggcg
     2641 ctgtcggtgc cgttgacgac ggcgatcgcg gcggtgctgg ccaagccggc cggaatcagc
     2701 tccgctccaa caactccagc agatagtcgc cgtacccgga cttgaccagg gtgcgcgccc
     2761 acgccgccag ctgctcgtcg gcgatccagc cctgccgcca ggcgacttcc tcggggacgc
     2821 tgaccttgag tccctgccgg cgttccagcg tgcgcacgaa atcgctggcg tccaacagcg
     2881 agtcgaacgt accggtgtcc agccaggccg tgccgcgggc catcacctcc acccccagcc
     2941 ggccccggtt gaggtagatc tggttgacct cggtgatctc gtactcaccg cgcggcgatt
     3001 tctggagtcc tttggcgatt tcgatcacgt cgttgtcgta gaagtacaaa cccggtacgg
     3061 cgtaattgga tttcggtgtt tcgggtttct cttccaacga cagcgccatg ccgtcggcgc
     3121 cgaattccac cacaccgtag gcggacgggt tggccaccca atacgcgaaa atggccccgc
     3181 cgcgcaggtt ttgaaagcgg ctcagactgg tgcccaaccc cgggccgtag aagatgttgt
     3241 cgcccaacac caaagccacc gattcggtgc caatgtggtt ggcgcccaag acgaacgcct
     3301 gcgccagacc gtccggctgt tcttgcatca cgtacgtgag gttgatgccg aattgcgagc
     3361 cgtcgccgag gagccgctga aagccggccg cgtcgtgggg ggtggtgatc accagaatgt
     3421 cgcggatccc ggccatcatc agagtggaca gcgggtagta caccattggt ttgtcataga
     3481 ccggaagcaa ctgcttgctg atgccgatcg tgatcggatg aaggcgggta cccgacccgc
     3541 cggccaaaat gatcccgcgc atatgcgcct cccctcccct aacttacgag gtcggcctcg
     3601 gtgagctcgg tcgctgccag cgccgacgcg agatcatggt gccggaacac cgaggtgacg
     3661 tggtcgtgga ccacccggaa cgccgacgcg gcgtcgctga cggtgccgtc cggggcggtg
     3721 accttctgtt cgacgaccac gacgccgtgg tggacgtaca tccggccagg ctccgccgtc
     3781 ccttcctggg aagcggccca cgtgcgcaac gcctggtggc cttgtgcggc tccgtgcgag
     3841 tcaccgatgt cgatgtcgtc actggacaag gccaccaagg tcgccaggtc ggaagcgttg
     3901 agggcgtcgt gccacgccag cacggtggcg atctccgatg tggtcatggt cagcaagtta
     3961 ccgtgtccgc cgcggctaga gcggggtacg gtccagccag ttctgccaca cggcggcgtc
     4021 gccgaagagc tcgataccgg tgtcggccag cgagacccgg cgcgtcatcg ccaggagcag
     4081 ctcggtggcg cccccgcgca gcgcgacgct gcccttgcca tgctcgtgcg accaggtgat
     4141 ttcgccccct tcgacaccga tggtccattc gccggcctcg cccagcccgg gatcgccggc
     4201 gtgcaagtgc acggtgttgc cctcctcgag cggcaatggc gtcccggcgc ttccggcctg
     4261 gatcgcgacc cgttcaagaa attcgctgat cccgtcggcg gcgatgccgg agtcgagcgt
     4321 gaagtcggtg ccggtcgcga tggcggcgtc ggcgcggtgc accgcggttt cgtgcagccg
     4381 gcgccggatc caccagttag ccgggcgcgg cccgaggaaa gtccacaccg gtgtttccgc
     4441 tcccgacagt tcgacagcgt cgacgagccg ctgcgcgccg ccatacagcc aggagatcgc
     4501 atcggcggga tccggcggcg gcttgccacc ctcgacggcg cggaaatcaa gcgcctggtc
     4561 gagcctgtcg cgcacgatct gcgccgccca gcggtcaccg cggccgacgt ggcgcaacag
     4621 ttgcgtcagg ctccaacccg ggcaggtcgg caccggcgta gacccgtcga cgtcccggaa
     4681 cagctccgcg aatgcccgat tctcgtcgag gaatgcgcca gcgtagtcca cgcggtcagg
     4741 ctacccgccg atgcgggctt gactgatcgg taatgcggcg ggccgcctat gacgataacg
     4801 tccattgggg ggacggtttt ggtgatggcg ggggccttcg cctgtcacga gctgacgaag
     4861 ggtgcgggat atgtcgtttg tgaacgcggc gccgggggcg ctggcgtcgg cggcttcgga
     4921 tttggctgga atcggtacga cgctcagtag cgcgagtgcg gccgcggccg ccccgacgac
     4981 cggcgtggtg gcctcggccg ccgacgaggt gtcggccgcg gtcgcatcgc tgttttccgg
     5041 ctacgcgcag gactttcagg agctgagcgc gcagacaacg gcgtttcatg accaatttgt
     5101 gcagacgttg agcgcgggcg cgggctcgta tgcgagcgcc gaggccgcca acgtccagca
     5161 gacgctgctg ggcgccatca acgcgcccag cgaggcgctg gtggggcgcc cgctgatcgg
     5221 taacggcgcc aacggggcgc cggggaccgg gcaaaacggc ggcaacgccg ggattttgat
     5281 cggcaacggc ggggccggcg ggtccggtgc acccggccag aacggcggca acggcggcgc
     5341 cggtgggctg ctcggcgccg ccggcgcagg cggcgccggg ggcgcttcca cgaccgggcc
     5401 cggcggaaac ggcggcaa
//
LOCUS       DASSGQ010000246         5314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1262528,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000246 DASSGQ010000000
VERSION     DASSGQ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262528"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFI55_10800"
     CDS             complement(<1..324)
                     /locus_tag="VFI55_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074553.1"
                     /translation="MITATLPSSFPTITPSGDYLAGEVKHLVKGGEQCEGIHDRFIST
                     GPGEALEPAGNRVNGRTRFEVDPHGRLQRVRIAAGVLRRTAGDRHKWRATSSAWTGRG
                     SAWTGG"
     gene            290..784
                     /locus_tag="VFI55_10805"
     CDS             290..784
                     /locus_tag="VFI55_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7074554.1"
                     /translation="MGKLDGKVAVITGGSSGMALAGAKLFVVEGAHVFISGRRQEALD
                     EAVNLLGPNVTGVQADSANLDDLDRLFDAVRREKAAVDVLWTSAGTGEERKLGEITEQ
                     HFDDTFGLNARGTVSTLHCGCRETAPAGSQVRADEIDRGIRQGLAAQLPGLPGPAQPP
                     QSRP"
     gene            851..1708
                     /locus_tag="VFI55_10810"
     CDS             851..1708
                     /locus_tag="VFI55_10810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET7074555.1"
                     /translation="MRIGVVFPQTELGGDPAVVRAYGQGVEQLGFTHILAYDHVVGAD
                     PTVHQGWQGPYDVDTTFHEPFVMFGYLAAVTTVELVTGIIILPQRQSVLVAKQAAEVD
                     LLTGGRFRLGIGLGWNPVEFEALGEQFANRGKRSEEQVELMRRLWTQRSVTFSGRYHT
                     VTGAGLAPMPTQQPIPVWFGAASDRAYERAGRLGDGWFPMMKPGPGLDYAREQVNRAA
                     ATAGRDANSLGMEGRASWAGDRDKVAADIAAWKAAGATHVSVNTMDAGLATVDDHLAA
                     LERVAADLK"
     gene            1954..3498
                     /locus_tag="VFI55_10815"
     CDS             1954..3498
                     /locus_tag="VFI55_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HET7074556.1"
                     /translation="MPAVTEIPRDHNPFPTAGVSRDVDGIPRYDDLPATLLDMLAEQV
                     DRRPGAQAVVELAAPGAERLTYRQLWDRASRVSGGLRAAGVQPGDRVAVRYPAGINWV
                     LAFWGTVMAGGVAVAVNTRSAQPEVEFVLSDSGARVDLASDAPLPDAHTYVTERLGRA
                     DTAALFYTSGTTGHPKGVPTTHEAFLTNTENALRCLEQPRDLGEAMRTLISVPLFHVT
                     GCNTQLLAAARLGGASVIMPALNLDELIAALTTERISVMVTVPAIYSLLLRHRAFASA
                     DVSGVRWVGYGGAPIAPSLVRAVKEAFPQATVFNGYGMTESASLMTALPDRDAVEHAD
                     SVGYAVPSVDLGLVPFGGDEPGVGELVARGANVAATYWNRPEATAATFADGWLHTGDV
                     VRVDDAGRVHIIDRLKDIINRGGENVSSVEVEAALLAAPTVADACVLAVPDDVMGEKV
                     GAVLFGGPEQVDVSSVLEHCRGQLADFKVPQYVTVVDEALPRNAGGKLLKGKLRKEVR
                     WGEPLR"
     gene            3495..>5314
                     /locus_tag="VFI55_10820"
     CDS             3495..>5314
                     /locus_tag="VFI55_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET7074557.1"
                     /translation="MSATLLIANRGEIALRIIRTATELGIQTVAVYAEDDAGSPHVHA
                     ADEAIGLPGTGPAAYLDQPAVLAAAANSGAGLIHPGYGFLSENPEFARAATAAGYTFV
                     GPHADVLELVGNKSSARGAAIAAGVPVLPATEGPSSVEDVRAFFAAQDGAIMIKALAG
                     GGGRGMRKVRSADQIDSAYQQCAAEARLGFGDPALFAEAALDAARHLEVQVVGAPAGH
                     QTRAWALGDRDCSIQRRYQKLVEIAPAQGLSDALRRGLHQAAARLCARVGLRGLATVE
                     FLVTGESFVFLEINPRIQVEHTVTEETTGVDLVAVQLAIAGGASYYQLGLPSGIASDG
                     DEVIGEPAARRGIAIQARVNMETFAADGSVVPAAGSLTVFSVPSGPGVRVDTYGRPGL
                     VTNPQYDSLLAKVITVVRGSSFPAAVRKARAALGEFGIEGIGTNLAFLRELLSDSQLQ
                     SGWVTTGFLDEKLPGLASATLSRQPDVRVTAVELYPGEEVLRAQLAGTVVEVAAEGVE
                     SDAGAPLIVLEAMKMQHVLAAPDALHIARRLVAPGQVVGTGDPLLVFTRSGVATDGES
                     RSSAVDLDRPRADLDEVRERHLLTLDAGRQAAVAKRHKQGR"
BASE COUNT          866 a         1764 c         1856 g          828 t
ORIGIN      
        1 gccgcccgtc cacgccgacc cgcggccggt ccaggcggac gaggtcgcgc gccacttgtg
       61 gcgatcgccc gccgtgcggc gcaggacgcc ggccgcgatc ctgacgcgct gaagacggcc
      121 atgcggatca acctcgaacc gggtacgtcc gttgactcgg ttgccggcag gctcgagcgc
      181 ctcgccgggt ccggtgctga tgaagcgatc gtggatgcct tcgcactgtt ccccaccctt
      241 gaccagatgc ttgacttcgc cagccaggta atcgcccgat ggagtgatcg tgggaaagct
      301 tgacggcaag gtggcggtga tcacgggcgg atcaagtggt atggcgcttg ccggcgccaa
      361 gttgttcgtt gtcgaaggag ctcacgtttt catctcgggt cgccggcagg aagctctgga
      421 tgaagccgtc aatctgctcg gcccgaacgt gactggcgtg caggctgatt cggccaacct
      481 ggacgatctg gaccgtttgt tcgacgcggt caggcgggaa aaggccgccg tcgacgtgtt
      541 gtggacaagt gccgggacgg gcgaggagcg caagctcggc gagatcacgg agcagcactt
      601 cgatgacact ttcgggctca acgcgcgcgg cacggtcagc accctgcact gcggctgtcg
      661 agagaccgca ccagccggca gccaggtgcg cgcagacgag atagatcggg gaatccggca
      721 gggtctcgct gcccagttgc cggggctgcc cggaccggct cagcctccgc agtcgcgacc
      781 gtgacctgag cgcggcccga gccgcgcgtt gtgcgggaag cagttggggt gcggcgtggt
      841 tgaaagccaa atgcgcattg gagtcgtgtt cccgcagacc gagctcggcg gtgaccccgc
      901 agtcgtccgc gcctacgggc agggcgtcga acaactcggg ttcacccaca tcctcgccta
      961 cgaccacgtc gtgggcgccg acccgacagt gcatcagggt tggcaaggcc cctatgacgt
     1021 cgacacaacg tttcacgagc ccttcgtcat gttcggctac ctggccgcgg tcaccacagt
     1081 ggaactcgtc accggcatca tcatcctgcc gcagcgacag tcggtgttgg tggccaagca
     1141 ggccgccgag gtcgatctgc tcaccggcgg gcgattccgg ctcggtattg gactgggctg
     1201 gaaccccgtc gagttcgagg cgctcggcga gcaattcgcc aaccgcggca agcgctccga
     1261 ggagcaggtc gagctgatgc gccggctgtg gacgcagcgt tcggtcacct tcagcgggag
     1321 ataccacacc gtgacggggg cgggcctggc tccgatgcct acccaacagc cgatccccgt
     1381 gtggtttggt gcggcttccg accgcgccta cgaacgcgcc ggacggctcg gcgacggctg
     1441 gttccccatg atgaaacccg gacccggcct cgactacgcg cgcgagcagg tgaaccgggc
     1501 ggcggcaacc gcgggccggg acgcgaacag cctggggatg gaaggccggg ccagctgggc
     1561 cggcgaccgc gacaaggtcg ctgccgacat cgcagcctgg aaggccgccg gggccacgca
     1621 tgtctcggtg aacaccatgg acgccggcct ggcgacagtc gacgaccacc tcgccgcctt
     1681 ggagcgggtc gctgccgacc tgaagtagcc gccccgaggg gcgcgccgaa gaggttgggc
     1741 gaaagtcgca gcgaccgtgg tggtttcgga caagccgagg acgcgccgtg gtatctagag
     1801 atacggcggc cagaacgcct cggcgggctg ggccaaggca gcgccgagca aataggactc
     1861 gccttcgacc gtgaagaacg cctcgggcat gatggcggaa tcctcgatcg tcaccccgca
     1921 tcacgatagg acaccggatc gtaaggacag ggcatgcctg cagtgaccga gattcctcgc
     1981 gaccacaacc cgttcccgac ggctggcgtt tcccgcgacg ttgatggcat tccgcgctac
     2041 gacgatctgc cggccacgtt gctggacatg ctcgccgagc aggtcgaccg ccgcccaggg
     2101 gcccaagccg tggtcgaatt ggcggcacca ggcgccgaac gactgaccta ccggcagctc
     2161 tgggaccgcg cgtcgcgcgt ctcaggaggg ctgcgcgcgg cgggtgtgca accgggcgat
     2221 cgcgtcgccg tgcgctaccc cgcggggatc aactgggtgc tggcattctg gggcaccgtg
     2281 atggccggcg gcgttgcggt ggcggtcaat acccgctctg cgcaaccgga ggtcgagttc
     2341 gtgctgtccg actcgggtgc acgggtggat ctggcgtccg atgcaccgct gcccgacgcc
     2401 cacacgtacg tgacggagcg gctcggtcgc gcggacaccg ccgccctgtt ctacacctca
     2461 ggcaccaccg gccatcccaa gggcgtgccg actacccacg aggcattcct gaccaacacg
     2521 gagaacgcgc tgcgatgtct ggaacagcca agggatctcg gcgaggcgat gcgcacgctc
     2581 atctcggtgc cgctgttcca tgtgaccggt tgcaacacac aacttctggc cgcagcccgg
     2641 ctcgggggag catcggtgat catgcccgcg ctcaacctgg acgagttgat cgccgcgctg
     2701 actaccgagc gcatctccgt catggtcacc gttccggcga tctattcatt gctgttgcgg
     2761 cacagggcat ttgcgagcgc cgacgtttcc ggcgtccgtt gggtcggcta tggaggcgcg
     2821 ccgatcgcgc cgtcattggt gcgggcggtg aaggaggcgt ttccgcaagc cacggtgttc
     2881 aacggatacg gcatgaccga atcggcatcg ctgatgaccg ctttgcccga ccgtgacgcc
     2941 gtcgaacacg ccgactcggt cggatacgcg gttccctcgg tggacctcgg tctggttccg
     3001 tttggcggcg acgagccggg cgtgggggag ttggtcgccc gcggagccaa tgtggcggca
     3061 acctactgga accggccgga ggcgaccgct gccactttcg cggacggctg gttgcacaca
     3121 ggagacgtgg ttcgggtgga cgacgcgggc cgggtgcaca tcatcgatcg gctcaaggac
     3181 atcatcaacc ggggcggcga gaacgtctcc agcgtcgagg tcgaagccgc cttgctggcg
     3241 gcgcccaccg tcgcggacgc ctgcgtgctg gcggtgcccg acgacgtcat gggagaaaag
     3301 gtcggcgccg tccttttcgg cggcccagaa caggtcgacg tgtcgtccgt gctcgaacac
     3361 tgccgcggcc agttggccga tttcaaggtt ccgcaatacg tcaccgtcgt ggacgaagcg
     3421 ttgccacgca acgccggcgg caaactgctc aagggcaagc tgcgcaagga agtccgatgg
     3481 ggtgagccgc tccgatgagt gcgacgctgc tgatcgccaa ccgtggcgag atcgcacttc
     3541 gaatcatccg caccgcaaca gaattgggca ttcaaaccgt tgccgtctac gccgaagacg
     3601 acgccggcag tccacacgtg cacgcagctg acgaagcgat cggtctgccg ggaaccgggc
     3661 cggccgccta cctggaccag cccgcagtgc tcgcggcggc cgcgaactcc ggcgccgggc
     3721 tgatccaccc tggctacgga ttcctcagcg agaaccccga attcgcccgc gccgcaaccg
     3781 cggccggcta cacgttcgtg gggccgcacg ccgatgtgct cgaattggtc ggcaacaagt
     3841 cgtcggcccg aggtgcggca attgccgccg gggttcctgt gctgccggcg accgaagggc
     3901 cgagcagcgt cgaggacgtg cgcgcattct tcgcagccca ggacggcgcc atcatgatca
     3961 aggcgctggc cggtggcggg ggccggggca tgcgaaaggt gcgcagcgcc gatcagatcg
     4021 acagcgccta ccaacagtgc gccgccgagg cccggctcgg cttcggtgat ccggcgctgt
     4081 tcgccgaggc ggccctcgac gccgcgcggc acctcgaggt ccaggtcgtg ggggcaccgg
     4141 ccggccatca gacacgcgcg tgggccctgg gtgatcggga ctgcagcatc cagcggcgct
     4201 accagaagct ggtcgaaatc gcacccgccc aagggctttc ggacgcgctg cgccgagggc
     4261 tacatcaggc tgcggcccgg ctgtgcgcca gggtcgggct gcgcggactg gccaccgtcg
     4321 aattcctggt caccggtgag agtttcgtct tcctcgagat caacccgcgc atccaggtgg
     4381 agcacacggt caccgaggag accaccgggg tggacctggt ggccgtccag ctggccatcg
     4441 ccggcggcgc gtcgtactac cagctgggac tcccgtcggg cattgcgtca gatggtgacg
     4501 aagtcatcgg cgagccggcc gcgcgacgcg gcatcgcgat ccaggcccgg gtcaacatgg
     4561 agacctttgc cgccgacggc tcggtggtgc ccgcggcggg ctccttgacc gtgttctcgg
     4621 tgccgagcgg gcccggagtg cgcgtcgaca cgtacggccg cccgggcctg gtcaccaacc
     4681 cgcagtacga ctcgctgctg gccaaggtca tcaccgtggt gcgcggatcg tcgtttccgg
     4741 cggcggtgcg caaggccagg gccgcgctgg gcgaattcgg catcgagggg atcggcacca
     4801 acctcgcgtt tctgcgggaa ctgctgtcgg acagtcagct tcaatcgggc tgggtgacca
     4861 cgggtttcct cgacgagaag cttcccgggc tggcgtccgc cacgttgtcc cgccagcccg
     4921 acgtccgtgt cacggcggtc gagctctacc ccggtgagga agtgctgcgc gcgcaattgg
     4981 ccggcaccgt ggtcgaggtg gcggccgaag gcgtcgagtc cgatgccggc gccccactga
     5041 tcgtcctcga ggcgatgaag atgcagcacg tgctcgccgc accggacgcg ctgcacatag
     5101 cgcgccgcct ggtggcgccc ggccaggtcg tcggaaccgg agacccgctg ctggtcttca
     5161 cccgtagcgg cgtcgcgacc gatggcgaat cacgttcgtc ggcagtggat ctcgatcggc
     5221 cgcgcgccga cctcgacgag gtccgagagc gacacctgct caccctcgac gcgggccggc
     5281 aggccgccgt cgccaagcgg cacaagcaag gtcg
//
LOCUS       DASSGQ010000247         9570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_593849,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000247 DASSGQ010000000
VERSION     DASSGQ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9570
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593849"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1788)
                     /locus_tag="VFI55_10825"
     CDS             complement(1..1788)
                     /locus_tag="VFI55_10825"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006867641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET7074558.1"
                     /translation="MLPTYFPSPPQGVWHLGPLPIRAYALFIITGIVAALMIGDRRWE
                     ARGGQRGVIYDVALWAVPFGLVGGRLYHLATDWQTYWGPGGAGFGAALRIWDGGLGIW
                     GAVALGGVGAWIGCRRHGIPLPAFADAIAPGIVLAQAIGRLGNYFNQELYGRETTVPW
                     GLEIFYRRDPSGYVDPHSLDGVSTGQIAFVVQPTFLYELIWDLLIFVALLYVDRRFKL
                     GYGRLFALYVAGYCVGRFGVELLRDDTATHIAGIRINVFTSTFVFIGAVVYIILAPKG
                     REDPETLRGTDEDAEESEESEESEPADEQATVASAATATANAVGGSDDEPHDSAEAVE
                     SAEVAEAEAAEPETVDTETVEAETEVVEPEGAEPDVAQSEAADAHVAEPETADAEAEE
                     SDVAADTEGEDVVSEVAEPGVAEAEELQADVADEPETEVEEPAQADTEALVADEPEAD
                     KPEAAEPETEVEELAQADTEALVAAVADAAEADEPEAAEPEVVEPETEVEEVAQADTE
                     APVADMADVADEPEAEAEVEEPAHDEAPTGSDVDAGDREDQPEAAPDTTPAADAETAT
                     EEAKADATTTPTGRARRRFGFLQRNRTRR"
     gene            complement(1791..2606)
                     /gene="trpA"
                     /locus_tag="VFI55_10830"
     CDS             complement(1791..2606)
                     /gene="trpA"
                     /locus_tag="VFI55_10830"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET7074559.1"
                     /translation="MTVEHSEAGRLGPTFDSCRNDNRAALIGYLPTGYPDVPTSVAAM
                     IALVESGCDIIEVGVPYSDPGMDGPTIARATEVALQGGVRVRDTLAAVEAISLAGGRA
                     VVMTYWNPVLRYGVDAFARDLAAAGGYGLITPDLIPDEAEQWLAASEEHRLDRIFLVA
                     PSSTPQRLVTTVEATRGFVYAASTMGVTGARDAVSQAAPELVSRVRAVSDIPVGVGLG
                     VRSREQAAQIGSYADGVIVGSALVSALGDSGLPALRVLTEELAIGVRQRAVAS"
     gene            complement(2603..3925)
                     /gene="trpB"
                     /locus_tag="VFI55_10835"
     CDS             complement(2603..3925)
                     /gene="trpB"
                     /locus_tag="VFI55_10835"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET7074560.1"
                     /translation="MVDLSRPKLPLSSAAIVEPTRHDPDAGGHFGGPGGYGGRYVAEA
                     LMAVIEEVTAAYEKERVNPDFLDTLDELQANYAGRPSPLYEAARLAEHAGSARIFLKR
                     EDLNHTGSHKINNVLGQALLAKRMGKTRVIAETGAGQHGVATATACALLGLDCVVYMG
                     AVDTARQALNVARMRLLGAEVISVETGSQTLKDAINDAFRDWVTNADNTYYCFGTAAG
                     PHPFPTMVRDFQRIIGLETRVQIQDQAGRLPDAVTACVGGGSNAIGIFHAFIDDPGVR
                     LIGFEAAGDGVETGRHAATFTGGSPGAFQGSYSYLLQDEDGQTIESHSISAGLDYPGV
                     GPEHAWLRHTGRAEYLPITDAEAMDAFRLLCRTEGIIPAIESAHAVAGALKLGPQLSE
                     VLGRDAVIVVNLSGRGDKDVETAAKWFGLVSPGEQGGQSKGPVDEGTQ"
     gene            complement(3968..4786)
                     /gene="trpC"
                     /locus_tag="VFI55_10840"
     CDS             complement(3968..4786)
                     /gene="trpC"
                     /locus_tag="VFI55_10840"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET7074561.1"
                     /translation="MSPASVLDSILEGVRADIAAREAVVSLSEIKAAAAAAPPPLDVM
                     AALREPGIGVIAEVKRASPSAGSLAPIADPAKLARAYEDGGARIISVLTEERCFRGSL
                     DDLDAVRAAVSIPVLRKDFVVQPYQIHEARAHGADMLLLIVAALNQSALVSMLDRTES
                     LGMTALVEVHTEQEADRALRAGANVIGVNARDLATLEVDRDCFARIAPGLPSKVIRIA
                     ESGVRGTGDLLAYAGAGADAVLVGEGLVKSGDPRAAVADLVTAGTHPSCPKPAR"
     gene            complement(4887..5576)
                     /locus_tag="VFI55_10845"
     CDS             complement(4887..5576)
                     /locus_tag="VFI55_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02234 family membrane protein"
                     /protein_id="HET7074562.1"
                     /translation="MADVRPNRPRRLTGRRKIGIAQLLLVVAAAGLWMAARLPWVVIR
                     SFDGLGPPKEVTLNGASWSTALLPLALLMLAAAVAALAVRGWPLRLLAGLLAAASLAV
                     AYLGISLWVIPDVTLRGADLAHISVMSLAGSERRYWGAGLAVAASACALIAAVLLMRS
                     ASDAGSARASATKYAAPASRRSNAGREEGGGTTLDEPETPEMSERMIWDALDEGRDPT
                     DRPRGSDTEGR"
     gene            complement(5569..7122)
                     /locus_tag="VFI55_10850"
     CDS             complement(5569..7122)
                     /locus_tag="VFI55_10850"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET7074563.1"
                     /translation="MHAHLAATTSRDEFRLLAAEHRVVPVTRKVLADTETPLSAYRKL
                     AANRPGTFLLESAENGRSWSRWSFVGAGAPSALTVRDGHAAWLGAVPRDAPTGGDPLQ
                     ALRTTLELLATHAPHSEPGLPPLSGGMVGFFAYDLVRRLERLPELVVDDLQLPDMVLL
                     LATDLAAVDHHEGTITLIANAVNWNGTDERIDEAYDDAITRLDVMTTALGQPLPSTVA
                     TFSRPEPRHRAQRTVEEYGKIVDYLVEQIAAGEAFQVVPSQRFEMDTDVDPIDVYRML
                     RATNPSPYMYLLHVPNDAGKTDFSIVGSSPEALVTVADGWAKTHPIAGTRWRGQTDEE
                     DQLLEKELLADEKERAEHLMLVDLGRNDLGRVCTPGTVRVDDYSHIERYSHVMHLVST
                     VTGMLDDGKTALDAVTACFPAGTLSGAPKVRAMELIEEVEKTRRGLYGGVVGYLDFAG
                     NADFAIAIRTTLMRDGIAYVQAGGGVVADSNGPYEYAEASNKARAVLNAIAAAETLAA
                     PAVSRRRNG"
     gene            7253..7717
                     /locus_tag="VFI55_10855"
     CDS             7253..7717
                     /locus_tag="VFI55_10855"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019737747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET7074564.1"
                     /translation="MKTGDTVADFELPDQTGTPRKLSALLADGPVVLFFYPAAMTPGC
                     TKEACHFRDLAAEFAAVGANRVGISADPVQKQAKFADMQKFDYPLLSDTEGTVATQFG
                     VKRGLLGKLMPVKRTTFVIDTDRRVLDVISSEFNMDTHADKALETLRARSSA"
     gene            complement(7730..8419)
                     /locus_tag="VFI55_10860"
     CDS             complement(7730..8419)
                     /locus_tag="VFI55_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074565.1"
                     /translation="MAEPQAMGRSISAAALDALSFEAPYVVERLVKDRVVDSVSEGER
                     LFTEAKRYLVLSEANPEADVGMYSVRVDEAWHTFLLYTDQYTQFCERFFGRYIGHAPT
                     NAPPDEAHDREHRPELTFCEFRERYEALFGEPLPDFWYDARGVNPARRMFNDSAGSMT
                     VVHHDSVAELLDDEGEVIVSANDIAYGALKFIAQTGAFYVRELPGGLTDKEKVALVEA
                     VMSEGALRLAP"
     gene            complement(8425..>9570)
                     /locus_tag="VFI55_10865"
     CDS             complement(8425..>9570)
                     /locus_tag="VFI55_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074566.1"
                     /translation="QHPGASGSGTSDIRAAGNVQGVHVKELPVEVSPRVQVESGMKLD
                     DLCALLDRTEFPAADAREHPERESVLSARDAVTTAVRDDEFLTDCMARELDVLEQRLP
                     RRGLVPFYTLPASGIGFAFGYWPPGRTAGAHEHTAWTITAVCRNQLVVQTFDREKSYR
                     RQELVPKNLFDAPAGRVGFIYEPCIHDPRNPTNRWSLSMHVTSPRDGENVSGDLCLPL
                     LDDMRAQRSVDYDDPYASVGWARYQHILIHQIVRFLAGTSVPAADDLLARCTHLGSAA
                     TRRYIDGLGRNKREKLAAAKTLTLTHQDLILSCREFDDCVALGVDTPNGWVEQVRMAR
                     LAREAIAFCASRAAFDVDEIPGRLTGEERWAIAEALEESGTFRIGAS"
BASE COUNT         1604 a         3330 c         3014 g         1622 t
ORIGIN      
        1 ctaacgacgc gttcggttcc tctgcaggaa gccgaatcga cgccgggcgc ggccggtcgg
       61 cgtcgtcgtc gcatcggcct tggcttcctc ggttgctgtt tcggcgtcgg cggcgggcgt
      121 cgtgtcgggc gcggcctcag gctggtcttc tcggtcccct gcgtcgacgt cggacccggt
      181 gggcgcttcg tcgtgcgcgg gctcctccac ctcggcttcg gcttcgggct cgtcggcaac
      241 gtcggccatg tcggctacag gcgcttccgt atccgcctgc gcgacctcct ccacctcggt
      301 ttcgggctcg acaacttcgg gttcggcagc ttcgggctcg tcggcttcgg ccgcgtcggc
      361 tacagcggcc acaagggctt ccgtatccgc ctgcgcgagc tcctccacct cggtttcggg
      421 ctcggcagcc tcaggcttgt cggcttcggg ctcgtcggcc acaagggctt ccgtatccgc
      481 ctgcgcgggc tcctccacct cggtttcagg ctcgtcggcc acgtcggctt ggagttcttc
      541 ggcctcagca actccaggct ccgcaacctc ggatacgacg tcttcgccct cggtgtccgc
      601 ggcgacgtcc gattcttcgg cttcagcgtc cgcggtttcg ggctcggcca cgtgggcgtc
      661 cgcggcctcg ctctgcgcta cgtcaggctc ggcgccttcg ggctccacga cttcggtttc
      721 ggcttcgacg gtctcggtgt cgacggtctc aggttccgca gcttccgcct cggcgacttc
      781 ggcagattcg actgcttccg ccgaatcatg cggttcgtcg tcactgcctc cgacagcgtt
      841 cgccgtagca gtcgccgccg aagcgacggt cgcctgctca tcggccggct cggattcttc
      901 ggattcttcg gattcttcgg catcctcgtc ggtcccgcgc agggtctcgg gatcctcgcg
      961 acccttgggc gccaaaatga tgtacaccac ggccccgatg aacacgaaag tcgatgtgaa
     1021 cacattgatc cggattccgg cgatgtgcgt ggccgtatcg tcgcgaagca gctcgacgcc
     1081 gaagcgtccg acgcagtaac ccgccacata gagcgcgaac agccgcccgt agcccaactt
     1141 gaaccgccgg tccacataga gcaatgcgac gaaaatcagc agatcccaga tcaattcata
     1201 cagaaacgtc ggttgcacga cgaacgcgat ctgccccgtc gagacgccgt cgagcgaatg
     1261 cgggtcgacg tatccggaag ggtctcgccg gtagaagatc tccaaacccc acggcaccgt
     1321 ggtctcgcga ccgtagagct cctgattgaa gtagttcccg agccgaccga tggcctgcgc
     1381 caggacgatt cccggggcga tggcgtcggc gaaggcgggc agcggaattc cgtgacgccg
     1441 acagccgatc cacgcgccga cgccgcccag ggctaccgca ccccagatgc ccaagccgcc
     1501 gtcccagatt cgcagcgccg caccgaaccc ggccccgccc gggccccaat aggtctgcca
     1561 gtcggtggcc aggtgataga gccggccgcc gaccaatccg aacggcacag cccacagcgc
     1621 gacgtcatag atgacgccgc gctggccgcc gcgtgcctcc cagcgccggt caccgatcat
     1681 cagagcggcc acgatgccgg tgatgatgaa cagcgcgtag gcgcgaatgg gcaacggccc
     1741 caggtgccac acgccttgcg gcgggctggg aaagtacgtg ggcaacatcg tcacgaggcg
     1801 accgcccgct gacgcacgcc gatggccagt tcttcagtca gcacgcgcaa tgccggcaag
     1861 ccgctgtcac ccagtgccga caccaacgcg gagccgacga tgacaccgtc ggcgtagctg
     1921 ccgatctgcg cggcctgttc tcgcgatcgc acccccaggc cgacgccgac gggtatgtcg
     1981 gacaccgctc ttaccctgct taccaattcg ggtgcggcct gcgacaccgc gtcacgggcg
     2041 ccggtcactc ccatggtcga cgccgcgtac acgaatccgc gcgttgcctc gaccgtcgtc
     2101 accaaccgtt ggggtgtcga ggacggtgcc accagaaata tgcgatccag ccgatgctcc
     2161 tcggacgccg ccagccactg ctcagcctcg tcggggatga ggtcgggagt gatcaggccg
     2221 tatccgccgg ccgccgccaa atcccgcgcg aacgcgtcaa ccccgtaacg cagcaccgga
     2281 ttccagtagg tcatgaccac agcacgtccg ccggccagac tgatcgcctc gaccgcggca
     2341 agcgtgtcgc ggacccgcac tcccccctgc agcgcgacct ccgtcgccct ggcaatggtc
     2401 gggccgtcca tgcccggatc cgaatacgga acaccgactt cgatgatgtc acaaccggat
     2461 tcgaccaggg cgatcatcgc ggccacggat gtcggcacat cgggatagcc ggtgggcagg
     2521 tagccgatca gcgccgcgcg attgtcgttg cggcaggaat cgaaggtggg cccgagcctg
     2581 ccggcttcgc tgtgctccac cgtcattgcg tgccctcgtc gaccggcccc ttcgattgcc
     2641 cgccttgctc gccgggcgaa acaaggccga accacttcgc ggcggtctcg acgtccttgt
     2701 caccgcgacc cgacaggttc accacaatga ccgcgtccct tcccaacact tcgctcagtt
     2761 gggggcctag cttgagggca ccggccaccg cgtgggcgga ttcgatcgcg gggatgatgc
     2821 cttccgtgcg gcacagcaga cggaacgcgt ccatcgcctc ggcatcggtg atgggcagat
     2881 attcggcgcg tccggtgtgc ctgagccagg cgtgttcggg acccaccccc ggatagtcca
     2941 gacccgctga aatcgaatgg gattcaatgg tctgaccgtc ctcgtcctgc agcagatagg
     3001 agtacgatcc ctggaacgcc cccggtgaac cgccggtgaa tgtcgctgcg tgcctgccgg
     3061 tttcgacacc atcgccggcc gcttcgaacc cgatcagccg cacgcccgga tcgtcgatga
     3121 acgcatggaa gatcccgatg gcgttggacc caccgccgac gcacgccgtg acggcgtcgg
     3181 gcaagcggcc cgcctgatcc tggatctgca cccgcgtctc caggccgatg atgcgctgaa
     3241 agtcacgcac catggtcggg aaaggatgcg ggccggccgc ggtgccgaag cagtagtacg
     3301 tgttgtcggc gttggtaacc caatcccgga acgcgtcatt gatggcgtcc ttcagcgttt
     3361 gcgaaccggt ctcgaccgag atgacctcgg cacccagcag ccgcatccgg gccacgttga
     3421 gcgcctggcg cgcggtgtcg acggcgccca tgtagaccac acaatccagc cccagcaacg
     3481 cgcatgcggt ggcggtggcc acgccgtgct ggccggctcc ggtttcggcg ataacccggg
     3541 tcttgcccat tcttttggcc aacaacgcct gacccagcac attgttgatc ttgtgagaac
     3601 cggtgtggtt caagtcttct cgtttgagga agatccgcgc cgagccggca tgctcagcga
     3661 gacgggcggc ctcatacaac ggtgacgggc ggcccgcgta attcgcctgc agctcatcga
     3721 gcgtgtccag gaaatccggg ttgacgcgtt ccttctcgta ggcggcggtg acttcttcga
     3781 tcacggccat caaggcctcg gcgacatagc ggccaccata accaccgggg ccgccgaaat
     3841 gaccacccgc atcgggatcg tggcgggtgg gttcgacgat ggccgcactc gaaagcggaa
     3901 gcttcgggcg ggacaggtct accatcacca attctcgacg cggggacggc tcatgcggtt
     3961 caaccgtcta gcgagccggt ttgggacagg acggatgggt gcccgcggtg accagatcgg
     4021 caaccgctgc gcgggggtcg ccacttttga ccagaccttc accgaccaat acggcgtcgg
     4081 cacccgcgcc ggcatacgcc agcaggtccc cggtgccacg caccccggac tcggcgatcc
     4141 tgatcacctt gctgggtagc ccgggagcga tgcgcgcgaa gcaatcccgg tccacctcga
     4201 gcgtcgcgag atcacgcgcg ttgacgccga tcacgttggc acccgcccgc agcgcccggt
     4261 cggcctcttg ctcggtgtgc acctccacga gggccgtcat gccaagggat tcggtgcggt
     4321 ccagcatcga caccaacgcc gactggttca acgccgcaac gatgagcaac aacatgtcgg
     4381 cgccgtgcgc gcgcgcctcg tggatctgat atggctgcac cacaaagtct ttgcgcagta
     4441 ccggaatcga gaccgcggcc cgcaccgcgt caaggtcgtc aagcgacccg cggaaacacc
     4501 gctcttcggt caaaacgctg atgatccgtg ccccaccgtc ttcgtaggca cgcgccagct
     4561 tcgccggatc ggcaatcggc gccagtgaac ccgccgacgg actagcgcgc ttgacctccg
     4621 cgatgacgcc gatgccgggc tcgcgcagcg cggccatcac gtccagcggc ggcggcgctg
     4681 ccgctgcggc cgctttgatc tcggacaggc tgacaacggc ttcgcgcgcg gcgatgtcgg
     4741 cccggactcc ctcgagaatg gagtcaagca ccgatgccgg gctcatacca gtcgtttcct
     4801 tcccactgcc accacactgt catcgcggtc actccgccga tttcgatgac gtcaatgaag
     4861 ggtagcgacc gtcggcgcgc gacccgtcac cgaccctcgg tgtcggaccc gcggggccgg
     4921 tcggtcgggt cgcggccctc gtcaagggcg tcccagatca tccgctccga catctccggt
     4981 gtctccggct cgtcgagcgt cgtcccgccg ccttcttcgc gtcccgcatt cgatcggcgg
     5041 ctcgcgggcg ccgcgtactt cgtcgcgctc gcgcgggccg accccgcatc cgaagccgac
     5101 cgcatcaaca gaaccgcagc gatgagagcg cacgccgagg cggccaccgc gagccccgcc
     5161 ccccaatagc gccgttcgct tccggccagc gacatcaccg agatgtgcgc cagatcggct
     5221 ccgcgcagcg tcacgtccgg aatcacccag agactgatac ccaggtaggc gaccgcgagg
     5281 ctggcggccg ccagcagccc cgccagcagg cgcagcggcc acccacgcac cgccagtgcc
     5341 gcgacggccg ccgccagcat cagcagcgcc aagggcagca gcgccgtcga ccacgacgcg
     5401 ccgttcaagg tcacttcctt cggcgggccc agcccgtcaa acgagcggat gacaacccac
     5461 ggcagccgcg ccgccatcca cagccccgcg gcggcgacca ccagcaacag ctgggctatc
     5521 ccgatcttcc gccggccggt gagccggcgg ggccggttcg gccggacgtc agccattgcg
     5581 gcgtcggctg acggccggag ccgccaacgt ctccgcggcg gcgatcgcgt tcagtaccgc
     5641 tcgcgccttg ttgctggcct cggcgtattc gtagggaccg ttggagtcgg ccactacccc
     5701 accgccggcc tggacatagg cgatgccgtc gcgcatcaac gtggtgcgga tggcgatcgc
     5761 gaagtcggcg ttgccggcga agtccaggta gccgaccaca ccgccgtaga ggccgcggcg
     5821 cgtcttctcc acctcttcga tcagctccat ggcccgcacc ttgggtgcgc ccgacagcgt
     5881 gccggccggg aagcaggccg tcaccgcgtc gagggcggtc ttgccgtcgt ccagcatccc
     5941 ggtcaccgtc gagaccaagt gcatcacgtg gctgtagcgc tcgatgtggc tgtagtcgtc
     6001 cacgcgcacg gtgcccggcg tgcagacccg cccaaggtcg ttgcgaccca agtcgaccag
     6061 catcaggtgc tcggcgcgtt ccttctcgtc ggccagcagc tccttttcca gcagctgatc
     6121 ctcctcgtcg gtttgcccgc gccatcgggt tccggcgatc ggatgcgttt tcgcccagcc
     6181 gtcggcgacg gtgaccagcg cctccgggct ggagccgacg atcgaaaagt cggttttgcc
     6241 agcatcattc ggcacatgca acagatacat gtaagggctt ggattggttg cgcgcagcat
     6301 ccgatacaca tcgatggggt cgacgtcggt gtccatctcg aagcgctgtg agggcaccac
     6361 ctggaaggct tcgccggctg cgatctgctc gacgagatag tcgacgatct tgccgtattc
     6421 ctcaacggtg cgctgggctc gatgcctggg ctcgggcctg ctgaaggtgg cgaccgtcga
     6481 tggcaacggc tgacccagtg cggtggtcat cacgtccaac cgcgtgatcg cgtcgtcgta
     6541 ggcctcgtcg atccgctcgt cggtgccgtt ccagttcacg gcgttggcga tcagcgtgat
     6601 ggttccctcg tggtggtcga cggccgccag atccgtggcc aggagcagca ccatgtccgg
     6661 cagctggagg tcgtccacca cgagttcggg cagccgttcc aggcgccgca ccaggtcata
     6721 ggcgaagaag ccgaccatgc cgccggacag cggcggtagc cccggctcgg agtggggggc
     6781 gtgggtggcc agcagctcca gggtggttcg cagggcctgc agcgggtcgc cgccggtggg
     6841 cgcgtcccgc ggcacggctc cgagccacgc ggcatggccg tcgcgcacgg tcagcgctga
     6901 cggcgcgccc gcgccgacga acgaccacct cgaccaggat cggccatttt cggcggactc
     6961 cagcagaaag gtgcccggcc ggttggcggc aagtttgcga taggccgaca aaggcgtctc
     7021 ggtgtcggcc aagaccttgc gggtcaccgg gaccacccgg tgctcggcgg ccagcaggcg
     7081 gaactcgtcc cgtgaggtgg tggcggcgag gtgggcatgc accgaaccat cctcgcagac
     7141 gtaagaaaag cgggccgcga gcgtaacgcc accgcgatct tcgtggctgg ttttcgcagc
     7201 ggcgttacgc tcgcgggcag ctcatcgggc ggacgggcgt agcgtggcgg ccatgaaaac
     7261 tggcgacacc gtggccgact tcgaacttcc cgaccagacg gggacgcccc gtaagctcag
     7321 cgcgctgctt gccgacgggc ccgtggtgct gttcttctac cccgccgcga tgacacccgg
     7381 ctgcaccaag gaagcctgtc acttccgcga cctcgccgcg gaattcgccg ccgtcggcgc
     7441 caaccgcgtc ggcatcagcg ccgatccggt gcagaagcag gccaaattcg ccgacatgca
     7501 gaagttcgac tacccgctgc tgtccgacac cgagggcacc gtcgccacgc agttcggcgt
     7561 caagcgcggc ctgctgggca agctgatgcc ggtcaaacgc acgacctttg tcatcgacac
     7621 cgaccgccgg gtgctcgacg tgatctccag cgaattcaac atggacaccc acgccgacaa
     7681 ggcgttggaa acgttgcgcg cgcggtcttc tgcctagcgc ttgcgcgagt tatggcgcca
     7741 agcggagcgc gccctccgac atgacggctt cgacgagagc gaccttttcc ttgtcggtca
     7801 gaccgcccgg gagttccctg acgtagaagg cgcccgtttg cgcgatgaac ttcagtgcgc
     7861 cgtaggcgat gtcgttcgcc gagacgatca cctcgccctc gtcatcgagg agctcagcca
     7921 ccgaatcatg gtgcaccaca gtcatgcttc ccgccgagtc gttgaacatc cgccgcgccg
     7981 ggttgacgcc gcgggcgtcg taccagaagt cgggcaacgg ctcgccgaac agcgcctcgt
     8041 acctctcgcg gaattcgcaa aacgtcaatt ccgggcgatg ctcgcggtcg tgcgcttcat
     8101 cgggcggtgc attcgtcgga gcgtgcccga tatacctgcc gaagaaccgc tcgcagaact
     8161 gtgtgtactg gtccgtgtac aggaggaacg tgtgccaggc ctcgtcgaca cgcactgagt
     8221 acatgccaac gtccgcttcc gggttggcct cggagagcac gagatatctc ttggcttccg
     8281 tgaacaacct ctcaccctcc gaaaccgagt cgacgaccct gtccttcacg aggcgctcga
     8341 cgacatacgg agcctcgaac gaaagcgcat cgagagccgc agccgaaatg gatcgcccca
     8401 tcgcttgcgg ttcagccacc gacatcagct ggctccgatt ctgaacgtgc cggactcctc
     8461 cagcgcttcg gcgatagccc agcgctcttc acccgtcagg cgccccggaa tctcatcgac
     8521 atcgaatgcc gccctgctgg cgcagaaggc gatcgcctcg cgggcaagtc gggccattct
     8581 cacctgctcc acccatccgt ttggcgtgtc gacacccagc gcaacgcaat catcgaattc
     8641 acgacaactc aagatcagat cctggtgggt gagcgtgagc gtctttgccg cggcgagctt
     8701 ctcacgtttg ttcctgccca agccgtcgat gtaccgacgg gtcgccgcgg agccgagatg
     8761 cgtgcatcga gccagcaggt catcggcggc cgggacactg gttccggcca ggaaacggac
     8821 gatctgatgg atcaggatgt gctgatacct ggcccacccg actgacgcgt acgggtcgtc
     8881 atagtccacc gatcgctggg ctctcatgtc gtccaatagc ggcaaacaca agtccccgct
     8941 gacattttcg ccgtcacgcg gactggtgac gtgcatcgac agcgaccacc gattcgtcgg
     9001 gttgcgcgga tcatgaatgc agggctcgta gatgaagccg acccggcctg ccggcgcgtc
     9061 gaagaggttc ttcgggacca gctcttgacg tcggtaggac ttctcgcgat caaaggtttg
     9121 caccaccagc tgattgcgac acacggcggt gatcgtccac gcggtgtgtt cgtgagcacc
     9181 cgcggttcgg ccgggcggcc agtagccgaa cgcgaacccg atacccgatg ccggcagcgt
     9241 gtagaaggga accaaccctc gacggggaag ccgctgctcc agcacgtcca actcgcgtgc
     9301 catgcagtcg gtcaagaact catcgtcacg caccgcggtg gtgacggcgt ctcgtgcgga
     9361 aagaaccgat tcccgctccg ggtgttcgcg cgcatccgcg gccgggaact ccgtgcggtc
     9421 gagaagcgca cacagatcgt cgagtttcat tccactttcg acttgcacgc gcggagacac
     9481 ttcgactggc aattctttga cgtgcacacc ttgcacgttt ccggcggcac gtatatcgga
     9541 ggtaccgctt cccgacgcac ctggatgttg
//
LOCUS       DASSGQ010000248         2239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_837814,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000248 DASSGQ010000000
VERSION     DASSGQ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837814"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VFI55_10870"
     CDS             complement(<1..568)
                     /locus_tag="VFI55_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HET7074567.1"
                     /translation="MDLILAAHGTRRPGGVAMIGDLAAQVSTMLDRRVQVAFVDVLGP
                     TPSEVLSRAAGGGRPAIVVPAFLSRGYHVRTDLPAHVAASGHPNVIVTSALGPSAEIA
                     RIVADQLAKSGWRLGDSVILGAAGTTDPRARADLHVIATTLSALVGSRVRLGFAAAGD
                     PHVDDAVADARRGGARRVVVASYLLADGL"
     gene            complement(559..1710)
                     /locus_tag="VFI55_10875"
     CDS             complement(559..1710)
                     /locus_tag="VFI55_10875"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HET7074568.1"
                     /translation="MAQPKSAPLTGYRVAVTSSRRAEELCALLSRQGAEVCSAPAINM
                     IALPDDDELHSNTKALIAEPPDILVAHTGIGFRGWLAAAEGWGLANELITALSSARIV
                     SRGPKATGALRAAGLHEEWSPESESSHEVLEYLLESGVAGMRIALQLHGAADAWDPFP
                     EFIGGLRFAGAEVVPIRVYRWKPTPLGGDFDQLVTGVARRQFDAVTFTSAPAAAAVLE
                     RGRELNIEDQLLGALRTDVHAMCVGPVTSKPLIRKGVPTSSPERMRLGALARHIAEEL
                     PLLGSCTVQAAGHLVDIRGTCVLVDGSITLLSPSGMAILRALAQRPGDVVARGDLLRV
                     LPGNSNDPHAVDTAVLRLRTALGDKNIIATVVKRGYRLALDDPPGSPWT"
     gene            complement(1788..>2239)
                     /locus_tag="VFI55_10880"
     CDS             complement(1788..>2239)
                     /locus_tag="VFI55_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074569.1"
                     /translation="RPKDFADGYTGVTVREGPATWPPQRFVGPLLGSLARIDGGRPAD
                     RDRGSRVTLGVLGGILLFVLSGMGYGSMFTMIPSIYEARSQGLDVGDAERRHWSYLDS
                     GTKTWAYRLFPAPYIVAAILTWMSYVRERVPAACMPADVLTTQPPRM"
BASE COUNT          383 a          771 c          748 g          337 t
ORIGIN      
        1 acaggccatc ggccagcaga taggaggcga ccacgacgcg acgcgcgccg ccacggcgag
       61 cgtcggcgac cgcgtcgtcc acgtgcggat cgccggcggc cgcgaatccc agccgcaccc
      121 gcgatccgac cagcgccgac aacgtcgtcg cgatgacgtg caggtcggcg cgtgctcggg
      181 gatccgtggt gcccgccgct ccaagaatca ccgaatcacc cagacgccaa ccggatttcg
      241 ccaactgatc ggcgacgatc cgggcaatct ccgcactcgg gcccagcgcg gaagtgacga
      301 tgacgttggg gtgtccactg gccgcgacgt gggcgggtag gtcggtgcgc acgtgatagc
      361 cgcgcgacag gaacgccggc accacgatgg cgggccggcc gccgcccgcc gcccgcgaca
      421 gcacctcgct gggtgtgggc cccaacacgt cgacgaatgc gacctggacc ctgcgatcga
      481 gcatcgtgct cacttgcgcc gcaaggtcgc cgatcatcgc gacgccgccg ggtctgcggg
      541 tgccgtgcgc ggccaggatc aggtccatgg ggaacccggc gggtcgtcga gggcaagccg
      601 gtaaccgcgc ttgaccaccg tcgcgatgat gtttttgtcg cccaaggctg ttcgcaacct
      661 cagcacggcg gtgtcgacgg cgtgcgggtc gttgctgttg ccgggcagca cccgtaacaa
      721 gtcgccacgt gcgaccacgt ccccgggtcg ctgcgcgagc gcgcgcagta tcgccatccc
      781 ggacggcgac agcagcgtga tcgaaccatc gaccagcaca cacgtgccgc ggatgtcgac
      841 caggtggccg gcggcctgga cggtgcacga ccccagcagc ggtagctcct cggcgatatg
      901 ccgggccagg gctcctaacc gcatccgctc cggtgacgac gttggaacgc ctttgcggat
      961 cagcggtttg gacgtcaccg gaccgacgca catcgcgtgc acatcggtac gcagcgctcc
     1021 caacagctgg tcctcgatgt tcagctcgcg accgcgttcc agcaccgcag ccgcggcggg
     1081 tgccgatgtg aaagtgaccg cgtcgaattg tcgtcgtgcc accccggtga ccagctgatc
     1141 gaaatccccg cccaatggtg ttggcttcca gcggtatacc cgaatgggca ccacctcggc
     1201 acccgcgaaa cgcaacccgc cgataaattc cgggaacggg tcccaggcgt cggcggcgcc
     1261 gtgcaactgg agggcgatgc gcattccggc gacacccgat tcgagcagat actccagcac
     1321 ttcgtgtgag gattcggact ccggagacca ttcctcgtgc aggccggcag cgcgcagcgc
     1381 tcccgtcgcc ttcggcccgc gcgacacgat ccgggccgac gacaacgccg tgatgagttc
     1441 gttggccagc ccccagccct cggccgcggc cagccagccg cgaaagccga tgccggtatg
     1501 cgccaccagg atgtcgggcg gctccgcgat caaagccttg gtgttgctgt gcagttcgtc
     1561 atcgtcggga agcgcgatca tgttgatcgc gggcgcgctg cagacctcgg cgccctggcg
     1621 gctcagcaat gcgcacagct cctcggcgcg gcgagaagac gtgaccgcga cccggtagcc
     1681 ggtcagtggc gcggattttg gctgggccat atgacctgtg taggcctggg gtgtttcgga
     1741 tgcgttacca cacaattgct gacgcattgc ccctgttgca gaagacatca catgcgggga
     1801 ggctgggttg taagcacgtc cgcgggcatg catgcggccg ggacccgctc gcgcacatac
     1861 gacatccacg tcaggatcgc ggcgacgatg tacggcgccg gaaacagcct atacgcccat
     1921 gtcttggtgc cgctgtcgag ataggaccag tggcggcgct cggcgtcgcc gacgtccagc
     1981 ccttgactgc gcgcctcgta gatcgacggg atcatcgtga acatcgatcc atagcccatc
     2041 cccgacagga cgaacagcag gatcccaccg agcacgccga gggtgacgcg gctaccgcgg
     2101 tcgcggtcgg ccggccggcc gccgtcgatg cgagccagtg atcccaacag cgggccgacg
     2161 aaacgttgcg gtggccatgt cgccgggcct tcgcgaacgg tcacgcccgt gtacccgtcg
     2221 gcgaaatcct ttgggcgcg
//
LOCUS       DASSGQ010000249        17610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1384875,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000249 DASSGQ010000000
VERSION     DASSGQ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 17610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17610
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384875"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VFI55_10885"
     CDS             <1..415
                     /locus_tag="VFI55_10885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7074570.1"
                     /translation="GLDPAKPTAWSAEGLLPFLPPDAQDALLDNIGVLSAAGSRLATE
                     NLPDASRSVPMMADRMREATDRWRDHGFDVEMTDLWYGGDRNDVLEYLNAHGWQAAAT
                     SAMDLFAAHGFTLPTAAEEGEAAFASLGYVTASRT"
     gene            complement(468..1181)
                     /locus_tag="VFI55_10890"
     CDS             complement(468..1181)
                     /locus_tag="VFI55_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid-like protein"
                     /protein_id="HET7074571.1"
                     /translation="MADASVGSRLRMMGLAVWRFVSGAPLTYAWLFVLMITTIIQNEL
                     PGRQLHAVLLHRSTNIHALGTDPLDVLFSSLLWIDGKNLEPYLLLFTLFLAPAEHWLG
                     QLRWLTVGLTAHVLATYVSEGLLYFAIEEHNAAQRLVHARDIGVSYFLVGVMAMLTYH
                     IARPWCWGYLGVLLVIFGFPLLTMDRVELNFTAIGHFTAILIGLCFYPMARANKKPQW
                     NPARFKQTVRRLRSHEAST"
     gene            complement(1194..3077)
                     /gene="glmS"
                     /locus_tag="VFI55_10895"
     CDS             complement(1194..3077)
                     /gene="glmS"
                     /locus_tag="VFI55_10895"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET7074572.1"
                     /translation="MCGIVGYVGPRPACQVVMDALRRMEYRGYDSSGIALVDGTGESG
                     KLTVRRRAGRLANLEEALAEMDPASLAGTTGLGHTRWATHGRPTDRNAHPHRDAAGKI
                     AVVHNGIIENFSALRHELEAAGVEFASDTDTEVAVHLVAQAYREGETAGDFVASVLAV
                     LRRLEGHFTLVFANADEPGTIVAARRSTPLVLGIGDGEMFVGSDVAAFIEHTRDAVEL
                     GQDQAVVITADGYRVTDFDGNDDLEPGEYREFHIDWDLAAAEKGGYEYFMLKEIAEQP
                     TAVADTLLGHFVDGRIVLDEQRLSDQELREIDKVFVVACGTAYHSGLLAKYAIEHWTR
                     LPVEAELASEFRYRDPVLDRSTLVVAISQSGETADTLEAVRHAKEQKAKVLAICNTNG
                     SQIPRECDAVLYTRAGPEIGVASTKTFLAQVTANYLVGLALAQARGTKYPDEVEREYQ
                     ELEAMPDLVARVLASVGPVADLARQFAQSSTVLFVGRHVGYPVALEGALKLKELAYMH
                     AEGFAAGELKHGPIALIEDGLPVIVVMPSPKGSAMLHSKLLSNIREIQSRGAVTIVIA
                     EEGDDTVRPFADHLIEIPSVSTLLQPLLSTIPLQVFAASVAQARGYDVDKPRNLAKSV
                     TVE"
     gene            3717..3947
                     /locus_tag="VFI55_10900"
     CDS             3717..3947
                     /locus_tag="VFI55_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv1535 family protein"
                     /protein_id="HET7074573.1"
                     /translation="MTAVLRDDAVAIPPEPPPTASSGAEPTAERATKRLLRSPIGAGS
                     DPLANAASHLLAVPLRQVYAVLWRMGLIEVKA"
     gene            4008..4847
                     /locus_tag="VFI55_10905"
     CDS             4008..4847
                     /locus_tag="VFI55_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074574.1"
                     /translation="MPRIRKLVAKLSHRGPHRVLHGDLAFAGLPGVVYTPEAGLNLPG
                     VAFGHDWLTGTARYSGLLEHLASWGIVAGAPNTQRGLAPSVLNLAFDLGTALDIVCGV
                     RLGPGNISVHPAKLGVAGHGFGASAAVFTAAGMPGKLAAVAALFPTVTDPPAEQPAAT
                     LKTPGLILSAPGDPTTLNSNALQLSQVWAAATLRIVSKAEVSGLAEGRRLTELVGLAG
                     PHRRTQRSVRALLTGYLLYTLGGDKEYRDFADPNVRLPRTDLVDPDAPPVTPEEKIVA
                     LLK"
     gene            complement(4950..6308)
                     /locus_tag="VFI55_10910"
     CDS             complement(4950..6308)
                     /locus_tag="VFI55_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003916549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074575.1"
                     /translation="MAVDRLDVAARLAEGRPAIEHTQSYVQACHALGYWQPDLTSRPS
                     QIRDWLDTEDGLDLRALDRDCDELREAGAAVMEALRIQRAQVAELAAAWTGPGGDAAV
                     GFLERHCDAAYTVATEVRTTAQRCESLGHNLWYLVDSKVATAIAIDDRTQAQRQTWLA
                     AAATVATGAGDRPTAEEVIRQQVNPYVDNDIRNDWLTTMRSTVAGIATSYNMVTDKMA
                     AVPAARFEFPGDLGPGYRPLPPAPPSAPASAAAVAPSVFPSLPVDSARAPTVTTAAPA
                     PAPTVPPTALPELGTALGDATAMPAGDLGGGLSGLGGLASRIVDAMGSLLGSAADRLG
                     DSAALDDSDVDPFRADDEHDDAEHDDDEHDNDEHDDAGNVGDPAQATGVDDSEAAMPT
                     AQTGPVDAPPPMDRLPVDARPAPVDARPVPVDASAPAADAPTEGSTPCEIAADQLPQA
                     GE"
     gene            complement(6308..6628)
                     /locus_tag="VFI55_10915"
     CDS             complement(6308..6628)
                     /locus_tag="VFI55_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007172522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion target"
                     /protein_id="HET7074576.1"
                     /translation="MRFDSRATFARTEVDVAAVHRVADQMVTAAEIIERAVGDHLTRL
                     TFSGATAGRAHAARGDVLRAQLERLVSDVSQWSRAAVEIAGALRTGAERYADAEMYAA
                     ARIA"
     gene            complement(6676..8013)
                     /gene="glmM"
                     /locus_tag="VFI55_10920"
     CDS             complement(6676..8013)
                     /gene="glmM"
                     /locus_tag="VFI55_10920"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET7074577.1"
                     /translation="MGRLFGTDGVRGVANRELTAELALALGAAAARRLATSGGPGRRI
                     AVIGRDPRASGEMLEAAVIAGLTSQGVDALRVGVLPTPAVAYLTGAYDADFGVMISAS
                     HNPMPDNGIKVFGPGGHKLDDDTEDQIEELVAAGPGLRPVGAGIGRAVDAEDAADRYL
                     RHLSKTGTLQLDGLTVVVDCAHGAASSVAPRAYRAAGARVIPINADPNGLNINDGCGS
                     THLDAVRAAVVAHRADLGLAHDGDADRCLAVDADGQLVDGDAIMVVLALAMQQAGELA
                     SDTLVTTVMSNLGLHLAMRSAGIAVRTTGVGDRYVLEELRAGKYSLGGEQSGHIVMPA
                     VGSTGDGIVTGLRLMTRMVQTGSSLADLASAMRALPQVLINVKVADKATAAAAPSVQN
                     AVEQAEAELGDTGRILLRPSGTEPMIRVMVEAPEEGLAQRIATGVAEVVSSAR"
     gene            complement(8165..8617)
                     /gene="rpsI"
                     /locus_tag="VFI55_10925"
     CDS             complement(8165..8617)
                     /gene="rpsI"
                     /locus_tag="VFI55_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019737895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET7074578.1"
                     /translation="MEVEVTEVVAEAPASAGDSFVFDRPIQTVGRRKEAVVRVRIVPG
                     TGKFDLNGRSLEAYFPNKVHQQLIKAPLVTVDRAETFDIYALLHGGGPSGQAGALRLG
                     IARALILAQPEDRPALKKAGFLTRDPRATERKKYGLKKARKAPQYSKR"
     gene            complement(8635..9078)
                     /gene="rplM"
                     /locus_tag="VFI55_10930"
     CDS             complement(8635..9078)
                     /gene="rplM"
                     /locus_tag="VFI55_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET7074579.1"
                     /translation="MPTYAPKAGDTTRSWYVIDATDVVLGRLAVAAATLLRGKHKPTF
                     APNVDGGDFVIVINADKVAISGDKLRQKQAFSHSGFPGGLHRRTIGELMQKHPTRVVE
                     KAIVGMLPKNKLSRDIQRKLRVYAGPAHPHTAQQPVPFEIKQVAQ"
     gene            complement(9342..9638)
                     /locus_tag="VFI55_10935"
     CDS             complement(9342..9638)
                     /locus_tag="VFI55_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HET7074580.1"
                     /translation="MDPVLSYDFGEIEYSVRQEIHTTSARFNAALDELKTQIAPLRQL
                     WTREAAVAYQAEQLKWHQAATALNEILVGLGNAVRDGAEQVADADRRAAGVWAR"
     gene            complement(9652..9969)
                     /locus_tag="VFI55_10940"
     CDS             complement(9652..9969)
                     /locus_tag="VFI55_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HET7074581.1"
                     /translation="MATPNTLSTDFDLMRSVADTTDARNDEIRAMLLAFIGRMSAVPP
                     AVWGGLAAARFKDVVDRWNAESLRLYHVLHTIADTIRHNEVTLQEAGQDHAHHIAAAG
                     GNL"
     gene            complement(10021..11280)
                     /locus_tag="VFI55_10945"
     CDS             complement(10021..11280)
                     /locus_tag="VFI55_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003917795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion-associated protein"
                     /protein_id="HET7074582.1"
                     /translation="MTAHLAIIEAGPASIRRLCCGTNRVADGEVSETVRQALEAIDDR
                     VALVDGRPVAVPALWRAALRSLNCPNSHAQNATVVVHPSWWSPARVGVVIAAARALAG
                     EVLARPRSWLLAQAADCDAVVVEIAERLVAITGATGAGSVAVPRGAQPRAVAEEVATV
                     IAGMTRDAAAAVLIDAPSSVAGAPALAASIAAALRDGGRAVAEIGDARLPPLAQAALS
                     VPDERSEPRSTAGSVRSRARVVAGLAGAAVVLTVLSLAISAVGTVDRRGASAPAQVQA
                     LPTTFLVEGRVAVTVPAAWSAQRVITGPGSARVQVTSPSDPEVALHVTQTPVAGETLS
                     GTAERLKRAIDGEPAGVFVDFNPAGTSGGRPAVTYREVRPGHHVRWTVLLDGPVRISI
                     GCQSRPGDEEAVRGACEQAVRSAHAIG"
     gene            complement(11277..15029)
                     /gene="eccCa"
                     /locus_tag="VFI55_10950"
     CDS             complement(11277..15029)
                     /gene="eccCa"
                     /locus_tag="VFI55_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014712333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccCa"
                     /protein_id="HET7074583.1"
                     /translation="MTQGFVPAVRRPPPVVAATDIAVDAPPGLPPAGSSGLLARLLPI
                     VMSVATLGVVAAAFFSGSAVARNPVYLALPAMVLASTAVTVVTGGGRRRGGGIDADRV
                     DYLGYLSRLRETVTETAAAQRSLLDWSHPEPAALWTLIGGPRMWERRTSDPDFCLVRV
                     GIATQPLAARLVAPEASAREPSDPVTAAALRRFIDAYSTIADAPMTFGLRGVEAVTID
                     GEPTMVRGLLRAMLCQLAVLHPPDQVLIVGAIGDRNRAHWEWLKWLPHNQHPTEADAM
                     GSARMVYQSLAETQRAVAEAGAPRVVVVADLDERATAGVIAGATLLEVGTGHDGTALI
                     ISQSGETQAVARPDRMDPIDALVCARRLAAYRVGAEGSGREDGPAWPKLAGLGDVVDF
                     DPITLWRSQVHRLCVPIGTTVDGAPLELDIKEPAERGIGPHGLCVGATGSGKSELLRT
                     IALGMMARNSPEALNLLLVDFKGGATFLDLARAPHVAAVITNLSEEAPLVARVRDALT
                     GEMDRRQQLLRTAGCASVAAYECARPTRGDLNALPTLFIIVDEFSELLSQHPDFADMF
                     VAIGRLGRSLGMHLLLASQRLDEGRLRGLEAHLSYRICLKTLSASESRTVLGTLDAYQ
                     LPNTPGAGFLRSSGGELIRFQAAFVSGPLRAQNPARVPTVPTEPASSVRVFSTRAVGP
                     VTRASETAGSTDGLTGGPPTGTVLHAVLDRLSGHGPPAHRVWLPPLDAAPPLETLLRE
                     VGSVPDLTVPIGIVDRPLEQRRTPLMVDLSGAAGNVAVVGAPQSGKSTALRTLIAALA
                     ATHDPALVQFYCLDFGGGALAALRTLPHVGAVAGRAEPRLVARVIAECESLVRSREAT
                     FADHGVDSIAHYRRLRAERSAVADDDPFGDVFLVVDGWAGFRRELEALETSIIALAAQ
                     GLSFGVHVVLSASRWADIRPSLKDQIGTRIELRLGDPADSEFDRKRALHVPRDRPGRG
                     LSRDGLHMAIAQPVDVVSDSESAAPPIPLLPNHVDHDAVVGQGGAELGTRILLGLEEL
                     GLQPVAVDFERHSHLLVLGDNECGKTATLRTLCREIVRTKPAAEAQLLIVDFRRSLLG
                     VVESEHLGGYAMSPTALNALLPDLLDVLRGRMPPLGASQAELRARSWWFGPDVYLVVD
                     DYDLVATPAGNPLAGILEYLPYAKDLGLHVVVARRSGGVERALFEPLLAGLRDLGCMS
                     LMMSGCPDKDAPFGSGPPARLPPGRGFLITRPGQEQLVQVAWSPPP"
     gene            15188..16552
                     /gene="eccD"
                     /locus_tag="VFI55_10955"
     CDS             15188..16552
                     /gene="eccD"
                     /locus_tag="VFI55_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion integral membrane protein
                     EccD"
                     /protein_id="HET7074584.1"
                     /translation="MSVHAGTAAVDLALPGGIPVAILIPSIVDLLGGNADPGNQEARR
                     YQLSRPGASALNTSTTLAQNGIPDGAVLVLTQSATPPPAPRYHDVAEAVSAALDATGR
                     PLSGVEQRRAARLTGAVAAICCTGVGVLALIRNAFITNGIRDAGGAAVVAAAAGLIAL
                     LGAGIAHRTHRDAINGLALSVIATAFAAVAGFLVVPGAPGIPNALLAATAAAATAVLA
                     RRVSGCGVVALNEVSCVAIVIAVAALAGMLTAASLRAVGSVSALISVGLLGLATRMSI
                     LLAGLSPQLPPAPELSTVEPGGACLAAKAIRADNWLAALRAGFCCSAAAGAVITVVAG
                     APRPSCIAFGAVTGASLLLRARCGDRGRTAVFVTAGITITATTFGVVTLGMPQHPAPI
                     AAATATLAAAAVYLGFVTPAAARSPVLRRGVEVLECVALATMVPLTCWICGFYGAVRA
                     LNLQ"
     gene            16549..>17610
                     /gene="mycP"
                     /locus_tag="VFI55_10960"
     CDS             16549..>17610
                     /gene="mycP"
                     /locus_tag="VFI55_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion-associated serine protease
                     mycosin"
                     /protein_id="HET7074585.1"
                     /translation="MSWSRAARLLAASALAALFELGTPVAQAVSPPQVDDRWLPKPAL
                     PAPPWPTVQREVCAVMTGSQPGRDRVADLMDLPRVWQLTRGAGQRVAVIDTGVSRHRR
                     LPDVVPGGDYVSTGDGTQDCDAHGTLVAGIIAATPDSSADGFSGVAPEATVISIRQSS
                     TKFAPAGDRSRTGVGDVDTMAKAIRTAADLGASVINISSVACVPVAATLDDRALGAAL
                     AYAVDVKNAVVVAAAGNTGGAGQCPPQRADATWQTVTVVASPAWYDDYVLTVGSLNAE
                     GTPSAFTLAGPWVDVAAPGEAVSSLGSEPVSGTSYAAPVVSGLAALIRARFPALTARQ
                     VMQRIESTAHHPPAGWDPVV"
BASE COUNT         2805 a         6111 c         5885 g         2809 t
ORIGIN      
        1 cgggttggac cccgccaaac cgaccgcgtg gagcgctgaa ggcctgctgc cattcctgcc
       61 gccggatgcc caggacgcgc tgctggacaa catcggcgtg ctcagcgcgg cgggcagccg
      121 attggcgacc gagaacctgc cggacgccag ccgttcggtc ccgatgatgg ccgatcggat
      181 gcgcgaggcg accgatcgtt ggcgcgacca cggcttcgac gtggagatga ccgacctgtg
      241 gtatggcggc gaccgcaacg atgtgctcga gtacctaaac gcccacggct ggcaggccgc
      301 tgccaccagc gccatggatc tctttgccgc ccacggcttt acgttgccaa cagccgccga
      361 agaaggggaa gcggcgttcg cctcgctggg ctacgtcacc gctagccgaa cctagccagg
      421 cggtgtcgaa tacgcttgcc ggacgacgac tttcgccgac gacccggtca ggttgatgcc
      481 tcgtgggatc ggagtcgccg cacggtctgc ttgaaccgcg cgggattcca ctgcggcttc
      541 ttgttcgccc gggccatcgg atagaagcac aaaccgatca ggatcgcggt gaagtgtccg
      601 atggcggtga agttcagctc gacgcggtcc atggtgagca atggaaaacc gaagatgacc
      661 agcagtaccc cgaggtaacc ccagcaccac ggtcgcgcga tgtgataggt cagcatggcc
      721 atcacgccga cgagaaaata actgaccccg atgtcgcggg cgtgcaccag ccgctgcgcg
      781 gcattgtgtt cctcgatcgc gaagtagagc aggccttcgc tgacgtaggt ggcaaggacg
      841 tgggcggtca atccgacggt gagccaacgc agttggccca gccagtgttc ggccggcgcg
      901 aggaacagcg tgaacagcag cagatagggt tccagattct tgccgtcgat ccacagcagg
      961 ctggaaaaca gcacgtccag cggatccgtc cccagcgcgt gaatgttggt ggagcggtgc
     1021 agcagcaccg cgtgcagttg ccgaccgggg agctcgtttt ggatgatcgt cgtgatcatc
     1081 agcacaaaca gccacgcgta ggtcaacggc gcgccgctga cgaaacgcca cacggcgagc
     1141 cccatcattc gcaggcggct acccaccgat gcgtccgcca cgagttccac gctctattcg
     1201 acggtgacag acttggccag gttccgcggt ttgtcgacgt cgtaaccgcg ggcctgcgcg
     1261 accgacgcgg cgaacacctg aagcgggatg gtcgacagca gcggttgcaa aagagttgac
     1321 accgaaggga tttcgatcaa gtggtcggcg aaagggcgta cggtgtcgtc gccctcttcg
     1381 gcgatcacga tcgtcaccgc gccgcggctc tggatctccc ggatattgga cagcagcttg
     1441 gagtgcagca tggccgaccc cttgggcgag ggcatcacca cgatgaccgg cagaccgtct
     1501 tcgatcagcg cgatcgggcc gtgcttgagc tcgccggcgg cgaacccctc ggcgtgcatg
     1561 taggcaagtt ccttgagttt gagcgcgccc tccagcgcca ccgggtagcc gacatggcga
     1621 cccacgaaca gcaccgtcga ggattgggcg aactgacgcg ctaaatcggc caccggcccg
     1681 acgctcgcaa gcacccgcgc gaccaggtcc ggcatcgcct ccagctcttg gtattcgcgc
     1741 tcgacctcat cggggtactt ggtgccgcgg gcctgcgcca gcgccaggcc gacgaggtag
     1801 ttggcggtga cctgggccag gaaggtcttg gttgaggcca caccgatctc cggtccggcg
     1861 cgggtgtaga gcaccgcatc gcattcgcgc gggatctgcg agccgttggt gttgcagatc
     1921 gccagcacct tggccttctg ctccttggca tgccggaccg cttccagggt gtccgcggtc
     1981 tcaccggatt gcgagatggc gacgaccaga gtgctgcggt ccagcacggg gtctcggtag
     2041 cggaattcgc tggcgagctc ggcttcgacc ggaagccgcg tccagtgctc gatcgcatat
     2101 ttcgcgagca agccggagtg ataggcggta ccgcacgcga ccacgaaaac tttgtcgatc
     2161 tcgcgtagct cctggtcgct caaccgctgt tcgtcgagca cgatgcggcc gtcgacgaaa
     2221 tgccccagca gcgtgtcggc caccgcggtg ggctgctcgg caatctcctt gagcatgaag
     2281 tactcgtagc cgcccttttc ggcggcggcc aggtcccagt cgatatggaa ctcgcggtac
     2341 tccccgggtt ccaagtcgtc gttgccgtcg aagtcggtga cccggtagcc gtccgcggtg
     2401 atcactacgg cctggtcctg gccgagttcg acggcgtcgc gggtgtgttc gatgaacgcc
     2461 gccacgtcgg agccgacgaa catctcgccg tcgccgatcc cgagcaccag aggcgtggag
     2521 cggcgcgcgg cgacgatggt gcccggctcg tcggcgttgg cgaacaccag ggtgaagtgg
     2581 ccctccagcc ggcgcagcac cgccagcacc gaggcgacga aatcgcccgc ggtctcgccc
     2641 tcgcggtagg cctgtgccac caggtggacc gcgacctcgg tgtcggtgtc gctggcgaac
     2701 tccacaccgg ccgcctccag ctcgtggcgc agggccgaaa agttctcgat gatgccgttg
     2761 tggacgacgg cgatttttcc ggcggcgtcg cggtgcgggt gcgcgttgcg gtcggtggga
     2821 cggccgtggg tggcccagcg ggtgtggccc aggccggtgg tgccggccag cgacgccggg
     2881 tccatttcgg ccagcgcctc ctccaagttg gccagccggc cggcgcgacg ccggacagtg
     2941 agtttgcccg actcacccgt gccgtcgacc agtgcgattc cggatgagtc gtagccgcgg
     3001 tactccattc ggcgaagcgc gtccatgacg acctggcagg ccggacgcgg tccgacgtag
     3061 ccgacgattc cgcacattct gaccagggta gtgcagaagg tcgtcgcacc ggacgtgcac
     3121 gactgaccgg gcggcgccgc ggccgaagcc aacgctcgtg gatccatttc aattcatgcc
     3181 atattcataa aacagcaact cttttcacct gatgtgcttg tgacgttttc gattgcgaca
     3241 tcgcgccaac tgggttcgca tttagacttc gaaaatatcg gctgggctgg caaccaccga
     3301 cgggtctcat gttctgcgaa ttcgccccaa ggaggctacg cattactacc ggtaggaaga
     3361 cggcatcggg agccagcggg aaggacgacg gtgttggcgc cagactgacc cgaatacgcg
     3421 aggtttgtga gcaactggtg aagacaaatc atgctgccgc acaacagatt aactgcgacg
     3481 cgagatcgcc gcataatcgc gcaaatcgtc attcgccgtc agttttatgc agcaggtatc
     3541 aattccatag tcgagcttgc cgatagccgc ggtgcggcac tgaaccaggg attcacgcgt
     3601 gcggatattc gcaccgccgc gcaaacctaa atattgagcg caatccggta gtacgccacg
     3661 gatgaaagaa cggtcttagg gcctgtaccc cacgcaccac ctcccaggag ggaaccatga
     3721 ctgcagtgct gcgcgacgat gcggtggcga tcccgcccga accaccgccg acggcatcgt
     3781 ccggggcaga gcccacggca gaacgggcaa cgaagcggct gctgcgctcg ccgatcggcg
     3841 ctgggagcga ccccttggcc aacgctgcga gccatttgct ggccgtaccg ctacgccagg
     3901 tctacgcggt gctctggcgg atgggtctga tcgaggtcaa ggcctaaatc aggccggtca
     3961 ccgttctgcc gacccggcgg accagtgtga tgcgctaacg tcgacgggtg ccccgcattc
     4021 ggaagctcgt cgccaagctg agtcaccgtg gcccgcatcg tgttttgcac ggggatctcg
     4081 cctttgccgg cctgccggga gtggtctaca cccccgaggc ggggttgaac ctgcctggtg
     4141 tcgcgttcgg ccacgattgg ctcaccggta ccgcccgcta ttcgggcctg ctggagcacc
     4201 tggcgtcgtg gggcatcgtg gccggcgcgc ccaacaccca acgcgggctg gccccctcgg
     4261 tgctcaacct ggcctttgac ctgggcacgg cgctggacat cgtatgcggt gtgcgcctcg
     4321 ggcccggcaa catcagcgtg catccggcca agttgggggt cgcgggacac ggcttcggcg
     4381 cctcggctgc ggtgttcacc gcggccggca tgccgggcaa gctcgccgcg gttgcggcgc
     4441 tctttcctac cgtgaccgat ccgccggccg agcagcccgc cgcaacgctg aagacgccgg
     4501 gtctgatcct gagcgccccc ggggatccca cgacgttgaa ttccaacgcc ctgcagctat
     4561 cccaggtctg ggccgccgcc acgttgcgca ttgtcagcaa agccgaagtc agtggtctgg
     4621 ccgagggccg gcgactgacc gagctggtcg ggctggccgg accgcaccgc cgcacgcagc
     4681 gctccgtccg cgctttgctg accggatacc tgctctacac gctcggcggc gacaaggaat
     4741 atcgcgactt cgccgatccg aacgtgcggc tgcccaggac ggatctggtg gaccccgacg
     4801 cgccacctgt cacgcccgaa gagaaaatcg tcgcgctgct gaagtgactc ggcgccggcc
     4861 aaaccgaacg gacccggcga gcccgcacga gcctggcgaa gccatctgct ttgtcgaaga
     4921 cgtactccag ccgctgtctg cctgagccct cactcccccg cctgcgggag ctggtctgcc
     4981 gcgatttcgc atggggttga tccctcggtc ggtgcgtcgg cggctggggc ggacgcgtca
     5041 accggtaccg gccgcgcgtc aaccggtgcc ggccgcgcgt ccaccgggag ccggtccatc
     5101 ggcggcggcg cgtcaaccgg cccagtctgt gcagtgggca tggccgcttc gctgtcgtcg
     5161 accccggtgg cctgtgccgg gtcgccgacg ttaccggcgt cgtcgtgctc atcgttgtcg
     5221 tgctcatcgt cgtcgtgctc ggcgtcgtcg tgctcgtcgt cagcgcggaa gggatccacg
     5281 tcggaatcat caagcgccgc cgagtcgccg agccggtcag ccgccgaacc cagcagcgaa
     5341 cccatcgcat cgacaatccg gctcgccagc ccgccaaggc cactcagtcc gccgcccaag
     5401 tcacccgccg gcatcgccgt cgcgtcacca agcgcggtgc ccagttccgg taacgccgtc
     5461 ggcgggacgg tgggcgccgg ggccggtgcc gcggtcgtca cggtcggcgc ccgcgcggaa
     5521 tccaccggca ggctgggaaa taccgacggc gcgaccgccg ctgccgacgc gggtgcactt
     5581 ggcggagccg gcggtagcgg tcgatagcca ggcccgagat cgcccggaaa ctcgaaacgc
     5641 gccgcgggca cggcggccat cttgtccgtg accatgttgt aggacgtcgc gatcccggcc
     5701 accgtcgacc gcatcgtggt cagccagtcg ttgcgaatgt cgttgtccac gtacggattt
     5761 acctgctgcc ggatcacctc ttcagcggtc gggcgatccc ccgccccggt cgcgaccgtg
     5821 gcggcggctg ccaaccacgt ctgacgctgt gcctgcgtgc gatcgtcgat ggcgatggcg
     5881 gtcgcgacct tggaatcgac cagataccag agattgtgac ccaatgactc acatcgttgg
     5941 gccgtcgtgc gcacttcggt ggccaccgtg taggcggcgt cgcaatgacg ctccaggaac
     6001 ccaacggcgg cgtcgccacc cggacccgtc cacgccgcgg ccagctcggc tacctgggca
     6061 cgctggatcc gcaacgcttc catgaccgca gcacccgctt cgcgcagttc gtcgcagtcg
     6121 cgatcgaggg cgcgcagatc gagaccgtct tcggtgtcca accagtcgcg gatctgggac
     6181 gggcgcgacg tcaggtcggg ttgccagtag ccgagcgcat ggcaagcctg cacgtaactc
     6241 tgggtgtgct cgatggcggg ccggccctcg gcaaggcgcg cggcgacgtc caatcggtcg
     6301 acggccatca ggcgatccgc gccgcggcat acatttcggc atcggcgtag cgctccgcgc
     6361 cagtccgcag cgcaccggcg atctcgacgg ccgcccgcga ccactgcgac acatcggaca
     6421 ccagtcgctc cagctgggcg cgcagcacat cgccccgcgc ggcgtgtgcc cggccggcag
     6481 tggcgccgct gaaagtcaac ctcgtcaggt gatctcccac cgcacgctcg atgatttcgg
     6541 cggcggtaac catctggtcg gcgacccggt ggactgccgc gacgtcgacc tcggtgcggg
     6601 caaaagtggc tcggctatcg aatctcatgc ctaatccgac gcccgacgcg gcgccggggt
     6661 tccgttatcg gcgcatcagc gcgcggagct caccacttcg gcaaccccag tggcgatccg
     6721 ctgcgcgaga ccttcctcgg gcgcctccac cataactcgg atcatcggtt cagttcccga
     6781 gggacgcaac aggattcggc cggtgtcgcc caattcggcc tcggcctgct ccaccgcgtt
     6841 ctgcaccgac ggcgccgcgg ccgcggtggc cttgtcggcg actttgacgt tgatcagcac
     6901 ctgcggcagc gcccgcatcg ccgaggcgag gtcggccagc gacgaaccgg tctgcaccat
     6961 cctggtcatc aagcgaagcc cggtgacgat gccgtcgccg gtggagccca ccgcgggcat
     7021 cacgatgtga ccggattgct cgccgccgag gctgtacttg ccggcccgca actcctccaa
     7081 aacgtagcgg tcaccgacgc cggtcgtgcg caccgcgatg ccggccgagc gcatggccag
     7141 atgcagcccg aggttgctca tcacggtggt caccagcgtg tcggaagcca gttcgccggc
     7201 ctgttgcatc gccagcgcca gcacgaccat gatcgcgtcg ccgtcgacga gttgcccgtc
     7261 ggcgtccacg gccaggcagc ggtcggcgtc cccgtcgtgc gccaggccca gatcggcacg
     7321 gtgggcaacg accgctgcac gcaccgcgtc gaggtgcgtc gagccacacc cgtcgttgat
     7381 gttgagcccg ttggggtcgg cgttgatcgg gatcacccgc gcaccggccg cgcggtaggc
     7441 gcgcggtgcc accgacgacg cggcgccatg ggcacagtcg accaccacgg taagcccgtc
     7501 cagctgcagg gtgccggtct tactgagatg gcgcaggtag cgatcggccg catcctcggc
     7561 gtcgacggcg cggccgatcc cggcgccgac cggacgcaac cccggtccgg cggcgaccag
     7621 ttcctcgatt tgatcctcgg tgtcgtcgtc cagtttgtgg ccgccggggc cgaagacctt
     7681 gatgccgttg tccggcatcg ggttgtgcga agccgagatc atcaccccga aatcggcgtc
     7741 ataagcaccg gtcaaatacg ccaccgctgg ggtcggaaga accccgaccc gcagcgcgtc
     7801 gacaccctga ctggtcaaac ccgcgatgac cgccgcttcc agcatctcgc cgctggcccg
     7861 cggatcgcgc ccgatcaccg cgatccgccg gcccggtccg ccggaagtcg ccaagcgccg
     7921 cgccgccgcc gcgcccagcg ccagcgccag ctcggcggtc aactcgcgat tggcgacccc
     7981 gcgaacaccg tcggtgccga atagacgacc catgcggaca aacctctcac agttggcggt
     8041 cgtgcaccta gacatgccac ttcttttctg accgaaaccg tgtgcgatcg tgcgcagaca
     8101 cgccgccgac ggcgtgcatc ttctgcccga agatgcacgg tcgcggccgg aaagtgatcg
     8161 ccgatcagcg cttgctgtac tgcggcgcct tgcgcgcctt cttcagcccg tacttcttac
     8221 gctcggtggc acgcgggtcg cgggtgagga agccggcctt cttcagggcg ggccggtcct
     8281 ccggctgggc caggatcagc gcccgggcga tgcccaggcg cagcgcacca gcctggcccg
     8341 acgggccgcc gccgtgcagc agggcgtaga tgtcgaaggt ttccgcccga tcgacggtga
     8401 ccagcggggc cttgatgagc tgctggtgca ccttgttggg gaagtaggcc tccaggctgc
     8461 ggccgttgag gtcgaacttg ccggtgccgg gcacgatgcg cacccgcacc acggcctcct
     8521 tgcggcggcc gacggtctgg atgggacggt cgaagacgaa cgaatcgccg gcactggccg
     8581 gcgcctcggc cacgacttcg gtgacctcga cttccacggt ctcggttgtc tcggtcactg
     8641 ggccacctgc ttgatctcga acggaaccgg ctgctgagcg gtgtgtggat gcgccggacc
     8701 ggcgtagacg cggagcttgc gctggatatc gcggctgagc ttgttcttgg gcagcatgcc
     8761 gacgatcgcc ttctccacga cacgggtggg gtgcttttgc atcagctcac cgatcgtgcg
     8821 tcggtgcagg cctccgggaa accccgagtg gctgaaggcc tgcttctgcc gcagcttgtc
     8881 gccgctgatg gcgaccttgt cggcgttgat gacgatgacg aagtcaccgc cgtcgacatt
     8941 gggggcgaac gtcggcttgt gcttgccgcg cagcagggtg gctgccgcga cggcaaggcg
     9001 gccaagcacc acgtccgtgg cgtcgatgac gtaccacgac cgcgtggtgt cacccgcctt
     9061 gggcgcatat gtgggcacag tgcttacctt cttctctttg cgagcctctt ggctcggggt
     9121 ggatcccggt gcgacccggg tgccggtcag gcgtccttgg tagatctgca gtagatctgc
     9181 ggtggggctt gatctcggcg accgacattg acccgagacc ccggcgtacc gcacgccaac
     9241 cgagcagctt accgacgtgg atccccgcag gtcaaaatga ctgtgtggtc ccagcggctc
     9301 tcctccgccg ggaccacaca ggcgctatct ggcgggcggc gctaccgggc ccagacgccg
     9361 gccgcccggc ggtcggcgtc ggccacctgc tccgcgccgt cccgcaccgc atttccgagg
     9421 ccgaccagta tctcgttgag cgccgtcgcc gcctggtgcc acttcagctg ctctgcctga
     9481 tatgcgaccg cggcttcgcg ggtccacagt tgtcgcagcg gggcgatctg cgttttcagc
     9541 tcgtccagcg ccgcgttgaa acgggccgat gtggtgtgaa tctcctggcg gacggagtat
     9601 tcgatttcgc cgaagtcata cgacaacacg gggtccacgg gtcgtccccc ttcagaggtt
     9661 cccgccggcg gcggcgatgt ggtgggcgtg atcctggccg gcctcctgca gcgtgacctc
     9721 gttgtgacgg atggtgtcgg cgatcgtgtg cagcacgtgg tagagcctca gcgactcggc
     9781 gttccagcga tctaccacgt ccttgaaccg cgcggccgcg agaccgcccc acaccgcggg
     9841 cggcaccgcg ctcatgcggc cgatgaacgc gagcagcatc gcccggatct cgtcgttgcg
     9901 ggcatcggtg gtgtccgcga ccgagcgcat gaggtcaaag tcggtgctca gtgtgtttgg
     9961 tgtcgccata ccaagttcga ccgcggcgac gtccgttcgg ttccacccga tttttccggc
    10021 tcatccgatc gcgtgggcgg accgcaccgc ctgttcgcag gcaccgcgga cggcctcctc
    10081 atcgccgggc cggctctggc agccgatgct gatccggacc gggccgtcca gcagtaccgt
    10141 ccaccgcacg tgatgaccgg gccgcacctc gcggtaggtc accgcgggcc ggccgccgct
    10201 ggtcccggcg ggattgaagt cgacgaagac tccggccggc tccccgtcga tcgcgcgctt
    10261 cagccgctcg gcggtgccgc tcagcgtctc gccggccacc ggcgtttgcg tgacgtgcag
    10321 agccacttcc ggatccgacg gcgacgtgac ctggactcgg gccgatccgg gcccggtgat
    10381 cacccgctgc gcggaccagg cagcgggcac cgtcacggcc acccggcctt ccaccagaaa
    10441 ggtggtcggc agggcctgca cttgggccgg ggctgatgct ccacgacggt ccacggtgcc
    10501 caccgccgag attgccaggg acaacaccgt cagcacaacg gcggcgcccg cgagcccggc
    10561 aaccacccga gcacgggatc gcacactgcc ggcggtggag cgcggctcgg accgctcatc
    10621 ggggaccgac agagccgctt gggccaacgg gggcagccgt gcgtcaccga tttcggcgac
    10681 cgcccggccg ccatcgcgca gcgccgcagc gatcgacgcc gcgagcgccg gcgccccggc
    10741 aacgctgctc ggcgcgtcga tcagcacggc cgccgcggca tctcgcgtca tcccggcgat
    10801 gaccgttgcg acctcctcgg caacagcacg cggttgagcg ccgcggggta cggcgacgga
    10861 acctgcaccg gtggcaccgg tgatcgccac caaccgttcg gcgatctcca ccaccaccgc
    10921 gtcgcaatcg gccgcctgcg ccagcagcca cgaccggggc cgggccagca cctcgccggc
    10981 cagggccctt gccgccgcga tcaccacgcc gacgcgcgcc ggcgaccacc acgaagggtg
    11041 caccaccacg gtcgcgttct gcgcgtgcga gttcgggcag ttcagtgatc gcagcgccgc
    11101 gcgccacagc gcggggaccg cgacgggtcg cccgtcgacc agcgcgaccc ggtcgtctat
    11161 cgcctccagc gcctgcctga cggtctccga cacttcgcca tcggcgaccc ggtttgtgcc
    11221 gcaacataac cggcgaatcg atgcggggcc ggcctcgatg atcgctaggt gtgcggtcac
    11281 gggggcgggc tccacgccac ctgaacaagc tgttcctggc cgggacgagt gatcaggaag
    11341 ccgcggcccg gcggcaaccg cgccggagga ccggaaccaa acggcgcatc cttgtccggg
    11401 catccactca tcatcagcga catgcagccg aggtcacgca ggcccgccag cagtggctcg
    11461 aacagggcgc gctccacgcc gccactgcgc cgcgccacca ccacatgcaa tccgagatcc
    11521 tttgcgtacg gcaggtattc gaggatcccc gccagtgggt tgcccgccgg cgtggcgacc
    11581 aggtcgtagt catcgaccac gagatacacg tccggtccga accaccagga cctggcgcgc
    11641 agctctgcct ggctcgcccc caacggcggc atccgcccgc gcaggacatc gagcagatcc
    11701 ggcagcaacg cgttcagcgc ggtcggcgac atggcatagc cgccaaggtg ttccgattcg
    11761 acgacgccga gcagggagcg gcggaagtcg acaatcagca gttgggcctc ggcggcgggc
    11821 ttggtccgaa cgatttcgcg gcacagcgtc cgtagcgtgg cggtcttccc gcattcgttg
    11881 tccccgagga ccaggagatg cgaatgtcgc tcgaaatcga ctgctacagg ctgcaatccc
    11941 aattcctcta ggccgaggag gattcgtgtt ccgagttcgg cgcctccttg gccgacgacg
    12001 gcgtcatggt ccacgtgatt aggcagaagc ggtatgggcg gtgcagcgga ctcgctgtct
    12061 gagaccacat cgacgggttg agcgatcgcc atgtgcagcc cgtcccggga cagcccgcgg
    12121 cccggccgat cgcggggtac gtgcaacgcc cgcttgcggt cgaactcgga gtctgcggga
    12181 tcgcccaacc gtagctcgat gcgagtgccg atctgatctt tgagcgacgg cctgatgtcg
    12241 gcccaacgcg atgccgacag caccacatgt acgccgaatg aaagtccctg ggccgccagg
    12301 gcgatgatcg acgtctcgag cgcctcaagc tcacgacgga agcccgccca gccgtcgacg
    12361 acgaggaaca cgtcgccgaa cgggtcgtcg tccgccacgg cgctgcgctc cgctcgcagc
    12421 cgacggtagt gggcgatcga gtcgacgccg tgatcagcga aggtggcctc gcgagatcgc
    12481 acgagcgact cgcattcggc gatcacacgc gccaccaacc gcggctcggc acgaccggcg
    12541 accgcaccca cgtgcggcaa cgtccgcagc gccgccagcg ccccaccgcc gaagtccagg
    12601 caatagaact gcaccagcgc cggatcgtgg gtggccgcca acgcggcgat gagcgtgcgc
    12661 agcgccgtcg acttacccga ttgaggcgcc ccgacgaccg ccacatttcc tgcggctccg
    12721 gacaagtcga ccatcagcgg cgtccggcgc tgctcgaggg ggcggtcgac gatgccgatg
    12781 ggtacggtca gatccggcac cgacccgact tcgcgcagca gggtttcgag tgggggtgcg
    12841 gcatcgagcg ggggcagcca gactcgatgt gcgggcggtc cgtgcccgga cagccggtcg
    12901 agaaccgcgt gcaggacggt ccctgtgggc ggtccgcctg tcaacccatc tgttgacccg
    12961 gctgtttcag aggcgcgggt gaccgggccg accgcccgtg tgctgaacac ccgcaccgaa
    13021 gatgccggct ccgtcggcac cgttgggacc cgagccgggt tctgcgcccg cagcggtccc
    13081 gaaacgaatg cggcctgaaa gcgaatcagc tcgccgccgc tcgagcgcag gaaacccgcg
    13141 ccgggcgtat tcggcaattg gtacgcgtcc agtgttccca gcaccgttcg tgattcgctc
    13201 gccgataggg tcttcaggca tatccgatag gacaggtggg cttccaggcc ccgcaaccgg
    13261 ccctcgtcga gccgctgact ggccagcaac agatgcatcc cgagcgaccg gccgagccgg
    13321 ccgatggcca cgaacatgtc ggcgaaatcg ggatgctggc tgagcaattc ggaaaactcg
    13381 tcgacaatga tgaacaacgt cggcagcgcg ttcaggtcgc cgcgggtcgg gcgcgcgcat
    13441 tcataggcgg cgacgctggc acagcccgca gttcgcagca actgttggcg ccggtccatc
    13501 tcgccggtca gtgcgtcgcg cacccgggcc accagcggcg cctcctccga taggttggtg
    13561 atgaccgcgg ccacgtgcgg agcccgggcc agatcgagaa acgttgcgcc tcccttgaaa
    13621 tcgaccaaca gcagattgag cgcctctgga gagtttcgcg ccatcatgcc cagcgcgatg
    13681 gtgcgtagca gctccgactt gcccgacccg gtcgcgccca cgcagagtcc gtgggggccg
    13741 ataccgcgtt cggcgggctc tttgatgtcc aactcgagcg gggcgccgtc gaccgtggtt
    13801 ccgatgggaa cgcacagccg gtgaacttgg ctgcgccaca gcgtgatcgg atcgaaatcg
    13861 acgacatccc caagcccggc gagcttgggc caggccgggc cgtcctcacg gccagaaccc
    13921 tccgcgccga cacggtacgc ggccagcctg cgcgcacaca ccaacgcgtc tatcgggtcc
    13981 atccggtcgg gacgagccac ggcttgagtt tcgccggact gactgatgat caacgcagtt
    14041 ccgtcgtgcc cggtaccgac ctcgaggagg gtcgcacccg cgatcacgcc ggccgtggcg
    14101 cgctcgtcca gatcggcaac caccaccacg cgcggcgcgc ccgcttcggc gaccgcgcgc
    14161 tgggtctccg ccagactctg atacaccatc cgagcagacc ccatcgcgtc agcctcggtg
    14221 ggatgttgat tgtgcggcaa ccatttcagc cactcccagt gggcgcgatt tcggtcgccg
    14281 atcgcgccga cgatcaacac ctggtccgga gggtgcagca ccgccagttg gcagagcatc
    14341 gcgcgcaaca gtcctcgcac catggttggc tcgccgtcga tcgtcacggc ctcaacgccg
    14401 cgcagcccga acgtcatcgg cgcgtccgcg atcgtcgaat acgcatcgat gaaacgacgc
    14461 aacgccgcgg cggtaacggg atccgaaggc tcccgcgcag acgcctcagg ggccaccaaa
    14521 cgggcggcaa gcggttgggt cgcgatgcca acgcggacca ggcagaagtc ggggtcggac
    14581 gttcgccgct cccacattcg cgggccgccg atcagcgtcc acagcgccgc gggctcgggg
    14641 tgactccagt cgagtaagga tctttgtgcc gcagctgttt cggtgaccgt ctcgcggagc
    14701 cgactgaggt atccgaggta gtcgactcgg tcggcgtcga taccgccgcc ccgccgccgg
    14761 ccgccaccgg tgaccaccgt caccgccgtt gacgccagca ccatcgccgg caacgccaga
    14821 tatacggggt tgcgggcgac cgccgaaccg gaaaagaatg ccgccgccac aacgcccagc
    14881 gtcgcgaccg acatcacaat cggcagcagg cgcgccagta ggccggatga tcccgccggg
    14941 ggaaggccgg gcggtgcgtc aacggcgata tccgtagcag cgaccaccgg cggcggccgc
    15001 cgcaccgcgg gcacgaaacc ctgagtcatc tccgccctcc taccgtggca cgacggtagg
    15061 cagggtcgaa atccgcggca agtcacctgt ggacagccga acaccgggga accgaaaact
    15121 ggctacggtg cggataatcc gtccgaaggg gtcaccgttg cccgcatccg atccggggtt
    15181 gcgccgagtg tcagttcatg ccggcacggc cgccgtcgac ttggccctgc ccggcgggat
    15241 tcccgtcgcc atcctcatcc cgtcgatcgt cgacctcctg ggcggcaacg ccgaccccgg
    15301 taatcaggag gcgaggcgtt accagctgtc caggcccgga gcgtccgctc tgaatacgtc
    15361 gacgacgttg gcgcagaacg gtatcccgga tggcgccgtt ctggttctga cgcaatccgc
    15421 taccccgccg cctgctcccc gttaccacga tgtggccgag gccgtttcgg cggcactgga
    15481 cgcgacgggc cggccgctga gcggcgtcga gcaacgccgg gcggcccgac tcaccggcgc
    15541 tgtcgcggcg atctgctgca ccggggtcgg tgtgttggcg ctcatccgaa atgccttcat
    15601 caccaacggc atccgcgatg cgggcggcgc cgccgtggtt gcggcggcgg cggggttgat
    15661 cgccctcctc ggtgcgggga ttgcacaccg gacccatcgg gacgcgatca acgggcttgc
    15721 actgagcgtc atcgcgaccg cattcgccgc agttgccgga tttctggtgg ttcccggcgc
    15781 ccccggcatc cccaacgcac tgctggccgc gacggccgca gccgccaccg cggtgctggc
    15841 gaggcgcgta tcgggctgcg gcgtcgtcgc attgaacgag gtctcgtgcg tcgcgatcgt
    15901 gatcgccgtt gccgcgctgg cgggcatgct caccgcggcc tcgctgcgcg ctgtcggctc
    15961 ggtatccgca ttgatctccg tcggtctgct cgggctggcg acgcggatgt cgatcctgct
    16021 cgcggggttg tcgccccaac tgccgcccgc accggagttg agcaccgtcg agccgggcgg
    16081 ggcttgcttg gccgccaagg ccatccgggc cgacaactgg ctggccgccc tgcgcgccgg
    16141 attctgctgt tccgccgccg ccggcgccgt catcaccgtg gtcgccggcg ctccacggcc
    16201 atcctgcatc gcttttggtg ccgtcaccgg cgcgtcgctg ctgttgcgtg ctcgttgcgg
    16261 tgaccggggc aggacggccg tgttcgtcac cgctggaatc accatcacgg caacgacgtt
    16321 cggggttgtc acgctgggca tgccgcagca cccggccccg atagccgctg cgaccgccac
    16381 actggccgcc gcggcggttt acctcggctt cgtcaccccg gccgcagcgc gatcacccgt
    16441 cctccgccgc ggcgtcgagg tgctcgagtg tgtggcgctg gccacgatgg tcccgttgac
    16501 ctgctggata tgcggcttct atggcgccgt acgtgcattg aatctccagt gagctggtca
    16561 cgcgcagcgc gcctgctggc ggcatcggcg ctcgccgcac tgttcgaact tggaacacca
    16621 gtggcgcaag cggtttcgcc gccgcaggtc gacgacaggt ggctacccaa gccggcgctg
    16681 cccgcgccgc cgtggccgac cgtgcaacgc gaggtgtgcg ccgtgatgac gggctcacag
    16741 ccgggccgtg accgggtcgc ggacctcatg gacctaccgc gggtctggca gctgactcgc
    16801 ggtgcgggac aacgggtggc ggtcatcgac accggcgttt cccggcatcg ccggctgccc
    16861 gacgtggtcc ccggagggga ctatgtgtcc accggcgacg gcacgcagga ttgcgacgcc
    16921 cacggcacgc tcgtagccgg aatcatcgct gccacgccgg attcctcggc cgacggcttc
    16981 agcggggtgg cgcccgaggc cacggtgatc agcattcgcc agtccagcac gaagttcgcg
    17041 cccgccggcg acaggtcccg caccggggtc ggcgacgtcg acaccatggc gaaagccatc
    17101 cggacggccg ccgatctcgg cgcgtcggtg atcaacattt cctcggtggc ctgcgttccg
    17161 gtcgcagcga cgctcgatga ccgcgcgctg ggggcggcgc tggcctacgc tgtcgacgtc
    17221 aagaacgccg tcgtcgtcgc cgccgcgggc aacaccggag gcgccgggca gtgcccgccc
    17281 cagcgggcgg atgcaacctg gcagaccgtg acggtcgtgg ccagtccggc ctggtacgac
    17341 gactatgtgc tgaccgtcgg ctcgctgaac gccgagggga cgccgtcggc gttcacgctc
    17401 gccggacctt gggtggacgt cgcggccccc ggcgaggcgg tcagttcgct cggttcggaa
    17461 ccggtatccg gcaccagtta cgcggcgccg gtggtcagtg ggctggccgc gctgatccgg
    17521 gcccggttcc cggcgctgac cgcacgacag gtgatgcagc gcatcgaatc gaccgcacat
    17581 cacccaccgg ccggctggga tcccgtcgtc
//
LOCUS       DASSGQ010000250         7631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_716183,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000250 DASSGQ010000000
VERSION     DASSGQ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7631
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716183"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..545)
                     /locus_tag="VFI55_10965"
     CDS             complement(15..545)
                     /locus_tag="VFI55_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7074586.1"
                     /translation="MSVRASAISILTEWRAPDAAQDALRHAVLAFVHGRDDACRRECA
                     PGHVTASALVLDDSGTQVLLTLHPRLGRWVQLGGHCDDEDADVVAAALREATEESGVT
                     GLRIEPELAALHVHPVTCSLGVPTRHLDMQFVVHAPSGAQIAISDESDDLRWWPADAL
                     PPGTDHALAYLVARAR"
     gene            complement(542..2167)
                     /locus_tag="VFI55_10970"
     CDS             complement(542..2167)
                     /locus_tag="VFI55_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7074587.1"
                     /translation="MPGICLSASDTWPMKTFAGKAVASADKVRGGYYTPGPVARFLAR
                     WVGEAGPRIVEPSCGDGRVLRELAELSAQVQGVELVAQEAAKSRTFAPVDAENLFTWL
                     AKTEPASWDGVAGNPPYIRFGNWAAEQRDPALELMRREGLRPSRLTNAWVPFVVAGTV
                     LVRDGGRVGLVLPAELLQVTYAAQLRDLLVSRFSEITLLTFERLVFDGIQQEVVLFCG
                     VVGAGPARIRTVQLSDADALSGADLADFQVESAPALLHEKEKWTKYFLDPAHIQLLRT
                     LKQSPKMLRLGSLADVDVGIVTGRNSFFTFTDAHADELGLRRHCVPLVSRSSQLSGLV
                     YDTDCRAGDVAAGHRTWLLDAPPDPTDPAVLAHIGAGEAAGVHRGYKCSIRHPWWTTP
                     SLWFPDLFMLRQIHRAPRLTVNAAAATSTDTVHRVRLASSVDPAALATVFHNSATFAF
                     AEIMGRSYGGGILELEPREAEQLPIPPPAYAGAELACDVDLLLKADEIEKALDVVDRH
                     VLVDGLGLSVEVVAACRAAWVSLRDRRTRRGSR"
     gene            complement(2139..3494)
                     /locus_tag="VFI55_10975"
     CDS             complement(2139..3494)
                     /locus_tag="VFI55_10975"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HET7074588.1"
                     /translation="MTGSGSTEHGTASTIEILPVTGLPEFRPGDDLSAAVAAAAPWLR
                     DGDVVVVTSKVVSKCEGRLVPAPQDAEERDRLRRKLVDEEAVRVLARKGRTLITENRL
                     GLVQAAAGVDGSNVGRDELALLPLDPDASAATLRAGLGERLGVDVAVVITDTMGRAWR
                     NGQIDAAVGAAGLAVLHSYSGAVDRFGNELVVTEVAVADEVAAAADLVKGKLTAMPVA
                     VVRGLNIADDGSTARQLVRPGDEDLFWLGTAEALDTGRRQAQLLRRSVRRFSDEPVPP
                     ELVEAAVAEALTAPAPHHTRPVRFVWLQTAATKTRLLDRMKDKWRVDLAGDGRPADSI
                     ERRVARGQILYDAPEVVIPFLVPEGAHAYPDAARTEAEHTMFTVAVGAAVQALLVALA
                     VRGVGSCWIGSTIFAADLVRAELGLPADWEPLGAIAIGYAEEPPGLRDSVDAGDLLVR
                     K"
     gene            complement(3491..4507)
                     /gene="cofD"
                     /locus_tag="VFI55_10980"
     CDS             complement(3491..4507)
                     /gene="cofD"
                     /locus_tag="VFI55_10980"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HET7074589.1"
                     /translation="MKVTVLVGGVGGARFLLGVQQLLGLGQFQPQQPQQQHVATNGHE
                     LTAVVNVGDDAWIHGLRVCPDLDTCMYTLGGGVDPQRGWGHRDETWHAKEELTRYGIQ
                     PDWFELGDRDLATHLVRTQMLRAGYPLSQITTALCDRWQPDARLLPATDDRCETHVVI
                     TDPADQSRRAIHFQEWWVRYRAQVPTHSFAHVGAEKASATTEVVAAIGDADVILLAPS
                     NPVVSVGAILAVPGIRGALRATTAPIVGYSPIIGGKPLRGMADACLSVIGVESTAQAV
                     GRHYGARGGTGILNCWLVHEGDEAEIEGVAVRSIPLLMSDPKATAEMVRAGLELAGLE
                     AGAA"
     gene            4895..5233
                     /locus_tag="VFI55_10985"
     CDS             4895..5233
                     /locus_tag="VFI55_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HET7074590.1"
                     /translation="MASTPHTTIGSAPAPIARPHLTVVPDAPVAFEPEPLPAPAADQW
                     QDRALCAQTDPEAFFPEKGGSTREAKKICLGCEVRHECLDYALAHDERFGIWGGLSER
                     ERRRLKRGIV"
     gene            complement(5252..5674)
                     /locus_tag="VFI55_10990"
     CDS             complement(5252..5674)
                     /locus_tag="VFI55_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HET7074591.1"
                     /translation="MRGPLLPPTVPGWRSRAERFDMAVLEAYEPIERRWQERVSELDV
                     AVDEIPRIAAKDPDSVQWPPEVIADGPIALARLIPAGVDVRGNATRARIVLFRKPIER
                     RAKDTVELGELLHEILVAQVAIYLDVEPTVIDPTIDDE"
     gene            5848..6315
                     /locus_tag="VFI55_10995"
     CDS             5848..6315
                     /locus_tag="VFI55_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 domain-containing protein"
                     /protein_id="HET7074592.1"
                     /translation="MNVPRRCCRPGCPHYAVATLTFVYSDSTAVVGPLATTREPHSWD
                     LCVGHAGRITAPRGWDLVRHAGPLPTHPDEDDLVALADAVREGGPTERSVPGFTGVPL
                     NGFPARVGGGAVDPQIQHAGAHATVPSSSLFAPPDHRSGRRRGHLRVLPDPSD"
     gene            6428..>7631
                     /locus_tag="VFI55_11000"
     CDS             6428..>7631
                     /locus_tag="VFI55_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HET7074593.1"
                     /translation="MSRPAASVHRVVKAYDVRGLVGDEIDESLVTDLGAAFAKLMRAE
                     GARQVVLGRDMRDSSPILAAAFATGVTGQGLDVVRIGLSSTDQLYFASGLLDCPGAMF
                     TASHNPAAYNGIKLCRAGAKPVGADTGLSVISDDVIAGVAKYEGQPGTIADRDLLDDY
                     GKFLRSLVDTSGLRPLRVAVDAGNGMAGHTAPAVLGAIGSITLLPLYFELDGSFPNHE
                     ANPLDPANLVDLQKFVGDVGADIGLAFDGDADRCFVVDERGRPVSPSTVTSLVAAREL
                     NREIGATVIHNVITSRAVPELVTERGGMPLRSRVGHSYIKGLMADTGAIFGGEHSAHY
                     YFREFWGADSGMLAALHVLAALGEQQRPLSELTADYQRYESSGEINFTVANASHCVDA
                     VLKSFGGRI"
BASE COUNT         1211 a         2650 c         2558 g         1212 t
ORIGIN      
        1 ggctggccgc acactcagcg cgcccgggcg accaggtagg ccagcgcgtg atcggttccc
       61 ggcggcagcg cgtcggcggg ccaccaccgc aggtcatccg actcgtcgct gatcgcgatc
      121 tgcgcgccgg acggcgcgtg caccacgaat tgcatgtcga gatggcgggt cggcacgccc
      181 agcgaacacg tgaccgggtg gacgtgcaga gcggccagtt cgggctcgat gcgcagaccg
      241 gtgacgccgg attcctcggt ggcctcgcgc aatgcggcgg cgacgacgtc ggcgtcttcg
      301 tcgtcgcagt gcccgcccag ctgtacccac ctgcccaacc gaggatgcaa ggtcagcaac
      361 acctgggtgc cgctgtcgtc gagcaccagc gccgacgcgg tgacgtggcc cggtgcgcac
      421 tcgcgacggc atgcgtcgtc gcggccgtgc acgaaggcca gcacggcgtg ccgcaacgca
      481 tcctgggccg cgtcgggggc gcgccattcg gtcaggatcg agatcgccga cgctcgcaca
      541 ctcaccggga accgcgcctt gttcggcggt cgcgcagcga aacccacgcc gctcggcatg
      601 ccgcaaccac ctcgaccgac aagccgaggc cgtcgaccag cacgtggcgg tccacgacgt
      661 cgagcgcctt ctcgatttcg tcggccttca gcaacagatc gacgtcgcag gcaagttcgg
      721 cacccgcgta cgccggtggg ggaatgggca gctgctcggc ttcccgcggt tcgagctcca
      781 agattccgcc gccgtagctg cggcccatga tctcggcgaa ggcgaacgtc gcgctgttgt
      841 ggaacaccgt ggccagtgcg gccggatcga cactggaggc cagtcggact cgatgcacgg
      901 tgtcggtgct cgtcgccgcg gcggcgttga cggtcagccg cggcgctcgg tggatctggc
      961 gcagcatgaa caggtctgga aaccacagcg agggggtggt ccaccacgga tggcggatcg
     1021 agcatttata gccccgatgc acgccggcgg cttcaccggc gccgatgtgc gcgagaacag
     1081 cgggatccgt cgggtcgggt ggcgcgtcca gcagccaggt gcggtggccg gccgcgacat
     1141 cgcccgcccg gcagtcggtg tcgtaaacga gtccggacag ttggctgctg cgtgaaacga
     1201 gcgggacgca atgccgcctg agtccaagct cgtcggcgtg cgcgtcggtg aaggtgaaga
     1261 agctgttgcg gcccgtcacg atgcccacat ccacgtcggc gagcgatccc agccggagca
     1321 ttttcgggga ttgcttgagc gtgcgcagga gctggatgtg agcggggtcc aggaagtact
     1381 tggtccactt ctccttttcg tgcagcagcg cgggggccga ctccacctga aaatccgcga
     1441 gatccgctcc cgaaagggcg tcggcgtcgc tgagctggac cgtgcggatc ctggccgggc
     1501 ccgcaccgac gacgccgcag aacaacacca cttcctgctg gatgccgtcg aacaccaatc
     1561 gctcgaatgt cagcaacgtg atctcgctga atcggctaac cagcaggtcg cgcaactggg
     1621 cggcgtaggt gacttggagc aattcggccg gcagcaccag gccgacgcgt ccgccgtcgc
     1681 gcaccaaaac cgtgcccgcg acgacgaacg gcacccacgc gttggtcagc ctgctgggcc
     1741 gcaggccctc gcgccgcatc aattccaaag ccgggtctcg ctgctcggcg gcccagttgc
     1801 cgaagcggat gtagggcggg ttgcccgcga ccccgtccca gctggcgggc tcggtcttgg
     1861 ccagccaggt gaacaggttc tccgcgtcaa cgggagcaaa cgtccgcgac ttggccgctt
     1921 cctgtgcgac gagttcgaca ccctggacct gggcgctgag ctcggccagc tcacgcagga
     1981 cgcggccgtc gccgcaggac ggctcgacaa tccgcggacc cgcttcgccg acccagcggg
     2041 ccaggaatcg ggccaccggg cccggggtgt agtagccgcc gcgaaccttg tcagccgacg
     2101 ccaccgcctt gcccgcgaac gtcttcatcg gccaagtatc acttgcggac aagcagatcc
     2161 ccggcatcga cggagtcgcg caacccgggc ggctcctccg catagccgat cgcgatggcg
     2221 cccaacggct cccagtcggc gggcagcccg agctctgcgc ggacgaggtc ggcggcgaag
     2281 atcgtcgagc cgatccagca gctgcccacc ccccgcaccg ccaacgcgac cagcaacgcc
     2341 tgcacggccg ctccgaccgc gaccgtgaac atggtgtgct cggcctcggt gcgggcggcg
     2401 tcggggtagg cgtgggcacc ctcgggcacc aggaacggga tgacgacctc cggtgcgtcg
     2461 tagaggatct ggccccgcgc cacccgccgc tctatcgagt cggcgggcct gccgtcgccg
     2521 gccaggtcga cgcgccactt gtccttcatg cggtccagca gccgggtctt ggtggccgcg
     2581 gtctgcagcc acacgaaccg caccggacgg gtgtggtggg gggccggcgc ggtcagggct
     2641 tccgcgacgg cggcctcgac cagctccggt ggcaccggct cgtcactgaa ccggcgcacc
     2701 gacctgcgca acagctgggc ttgccggcgg cccgtatcga gggcttcggc ggtgccgagc
     2761 cagaacaggt cctcgtcgcc cggccgcacc agttgccggg ccgtcgagcc gtcgtcggca
     2821 atgttcagcc cgcgcaccac ggccaccggc atggcggtca atttgccctt caccagatcc
     2881 gcggccgccg cgacttcgtc ggcgaccgcg acctcggtga ccaccagctc gttgccgaat
     2941 cggtcgacgg ctcccgaata gctgtgcagc acagccagac ccgccgcgcc gacggcggcg
     3001 tcgatctggc cgttgcgcca ggcgcggccc atcgtgtcgg tgatgaccac ggcgacgtcg
     3061 acaccgagtc gctcacccag cccggcgcgc agggtcgcgg cgctggcgtc ggggtcgagc
     3121 ggcagcagtg ccaattcgtc gcggccgaca ttggatccgt ccaccccggc ggcggcctgg
     3181 accaggccga gccgattctc ggtgatcagg gttcggccct tgcgtgccag cacgcgcacg
     3241 gcctcctcgt cgaccagctt gcggcgcagc cgatcccgct cctcggcatc ctgcggtgcc
     3301 ggaaccaggc ggccctcgca cttggacacc accttgctgg tcaccacgac gacgtccccg
     3361 tcgcgcagcc acggtgcggc cgcggcgacg gccgcactca gatcgtcacc gggccggaat
     3421 tcgggaagcc cggtgaccgg caggatctcg atcgtcgacg ccgtgccgtg ctcggtcgaa
     3481 cctgagccgg tcacgccgct cccgcctcca ggcccgcaag ctcgagcccg gcgcggacca
     3541 tctcggccgt cgccttcggg tcgctcatca gcagtggtat cgaacgcacc gcgaccccct
     3601 cgatttcggc ctcgtcgccc tcgtgcacca gccagcaatt cagaatcccg gtgccgcccc
     3661 gcgccccgta atgccggcca accgcctgcg cggtggactc gaccccgatc acggaaaggc
     3721 acgcatcggc catgccacgc aacggctttc caccgatgat cggtgaatag ccgacgatcg
     3781 gcgccgtggt ggcccgcagc gctccgcgaa tgccggggac ggccaggatg gcgccgacgc
     3841 tgaccaccgg gttggacggt gccagcagga tcacgtcggc gtcgccgatc gccgccacca
     3901 cttcggttgt ggcgctggcc ttttcggcgc ccacgtgcgc gaagctgtgg gtgggcacct
     3961 gagcccggta gcggacccac cactcctgga agtggattgc ccgacggctt tggtcggccg
     4021 gatcggtgat caccacgtgc gtctcgcacc ggtcgtcggt ggcgggcaac aaccgtgcgt
     4081 ccggttgcca gcggtcacac agcgccgtgg tgatctgtga cagcgggtag cccgctcgca
     4141 gcatctgggt gcgcaccaga tgggtggcca gatcgcggtc gcccagctcg aaccagtccg
     4201 gctggatgcc gtagcgcgtc aattcctctt tggcgtgcca ggtttcgtcg cggtgacccc
     4261 acccgcgctg cgggtcgacc cctccaccta aggtgtacat gcaggtgtcc agatccggac
     4321 agacccgcag cccgtggatc caggcgtcat cgccgacgtt gacaaccgcg gttagctcgt
     4381 gaccgttggt cgcgacatgt tgttgctgtg gctgttgtgg ttgaaactgc cccagcccca
     4441 gcagctgctg gacccctagc aggaagcggg cgccgccgac cccaccgacg agaacggtga
     4501 ccttcacgtt gcttgacagt actgcccgac gacgatgcgg tccgccggga gccattcatg
     4561 cgacacgaaa cggccacgaa acggccacga gactgccgcg aaagagcgct gcgagggctg
     4621 tgatcgacac gccgaggggc aattacaaca gaaacgccga aacttagtca cgagatgata
     4681 tggaaatgcg gcgtaacgct tgacccagca ggtcaacccg tgtctaatca catcagtgtc
     4741 atttcccggt tggccggccg gttccggtgt cgcagaccga gattcgatca cttgttcgaa
     4801 ttgtctgtat atcagaacag cgggaaacat tcactctctc aaatcaaggc gaggaggcgg
     4861 acgacgcatg tcctatgagc accttcgggg cgttatggca agcacaccgc acaccactat
     4921 agggtcggcg cccgcaccaa ttgcgcggcc gcatttgacc gtggttcccg atgcgccagt
     4981 cgcattcgaa cccgagccct tgccggcacc ggccgcggac cagtggcagg accgcgcact
     5041 gtgcgcgcag acggaccccg aggccttctt tccggagaag ggtggctcga cccgtgaggc
     5101 gaagaagatc tgcctgggtt gcgaggtgcg tcacgagtgc ctggactacg ccttggcgca
     5161 tgacgagcgc ttcggcatct ggggcggtct ctcggaacga gaacgccgcc gcctcaagcg
     5221 cggcatagtc taactcgacc gcagcccaac gttattcgtc gtcgatcgtc gggtcgatga
     5281 ccgtgggctc gacgtccagg tagatcgcca cctgagccac cagtatctcg tgcaacaact
     5341 cgccaagttc gacggtgtcc ttcgctcgtc gctcaattgg cttacggaat aagacaattc
     5401 gtgcgcgtgt ggcgttgccg cggacgtcga cgcccgccgg gatcaagcgg gccagcgcga
     5461 tcgggccgtc ggcgatgacc tcgggcggcc actgcacact gtcgggatcc ttggcagcga
     5521 tccgggggat ttcgtcgacg gccacgtcga gttccgacac ccgctcctgc cagcgccgct
     5581 cgatcggttc gtaggcctcc agcaccgcca tgtcgaaccg ctctgcccgg ctgcgccatc
     5641 ccggcaccgt cggcggcagc agcggaccgc gcatctcgcg gccccttcgg gaggctcgca
     5701 ccgaagccga ccgatgccgc caccgatcgc tttgcgagga gccgcgcgaa tcgcccacgc
     5761 gccgatggta acggttgcac atcgcggctc gagcggatgc gcgtgtccgg cgcttcggcg
     5821 gcgtttccgc acgatagcct ttcggtcgtg aacgttcccc gtcgctgctg ccggcctggg
     5881 tgtccgcact atgcggtggc gaccttgacg ttcgtctact cggattcgac agcggtggtg
     5941 gggccgctcg ctacgacgcg cgaaccgcat tcttgggacc tctgcgtcgg ccacgccggc
     6001 cggatcaccg cgccccgcgg gtgggacctg gtgcggcacg ccggaccgct gcccacccat
     6061 cccgacgagg acgacctggt cgccctggcc gatgcggtgc gcgagggcgg tccgaccgaa
     6121 cgtagcgtgc ccgggttcac cggcgtgcct ctgaacggct ttcccgcgag agtgggtggt
     6181 ggcgccgtcg atccgcagat tcagcacgcc ggggcgcatg ccaccgtgcc gagcagcagc
     6241 ctgttcgccc cacccgatca tcgttcgggg cgccgccgcg gacatctgcg ggtgttgccc
     6301 gaccccagtg actagccgtg actagcgcgg gtgtgcaacc cccgcccacc ggtccggtcc
     6361 gtattccaaa cgggccgata ggctgacgtc aacaggcaag ggatcgatgg cgtcaggagg
     6421 cccccacatg tctcggcccg ccgcgagtgt ccaccgcgtc gtcaaggcat acgacgtgcg
     6481 cgggctggtc ggcgacgaga tcgacgagtc gctggtcacc gatctggggg ccgcattcgc
     6541 caagttgatg cgtgccgagg gtgcgcggca ggtcgtgctc ggccgcgata tgcgggacag
     6601 ttcgccgata ctggccgccg cgttcgccac cggggtcacc ggccagggcc tggatgtggt
     6661 gcgaatcggt ttgtcgtcca ccgatcagct ctatttcgcc tcggggttgc tggattgccc
     6721 cggcgccatg ttcaccgcaa gccacaaccc ggcggcctac aacggcatca agctgtgccg
     6781 agcgggcgcc aaacccgtcg gcgctgacac cgggctgagc gtcatcagcg acgatgtgat
     6841 agccggggtc gcgaagtatg aagggcaacc cggaaccatc gccgatcggg atctgctgga
     6901 cgactacggg aagttcctgc gttcgctggt ggacacgtcc gggctgcgtc ccctgcgggt
     6961 ggccgtcgac gcgggcaacg gcatggccgg tcacaccgcg cccgccgtgc tcggcgcgat
     7021 cggctcgatc accttgttgc cgttgtactt cgaactcgac ggctcgttcc ccaatcacga
     7081 ggccaaccca ctcgacccgg ccaacctggt tgacctgcag aaattcgtcg gggacgtcgg
     7141 tgccgacatc gggctggcct tcgacgggga cgccgaccgg tgcttcgtcg tcgacgagcg
     7201 cggccggccg gtgtcaccgt cgacggtgac aagcctggtg gccgcacgtg agctcaaccg
     7261 cgagatcggc gccaccgtca tccacaacgt gatcacttcg cgcgcggtgc ccgaactggt
     7321 caccgagcgc ggcggcatgc cgctgcgctc acgggtcggg cactcctaca tcaaggggct
     7381 gatggccgac accggtgcga tcttcggcgg tgagcattcg gcgcactatt acttccgtga
     7441 attctggggc gccgactccg gcatgctggc ggcgctccat gtgctggccg ccctcggtga
     7501 gcagcagcgg ccgctgtcgg agctgactgc cgactatcag cgctacgaat cttcgggcga
     7561 gatcaacttc acggtggcga acgcgtcgca ttgcgtggac gcggtgctga agtctttcgg
     7621 cggccggatt c
//
LOCUS       DASSGQ010000251        27265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_17373, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000251 DASSGQ010000000
VERSION     DASSGQ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 27265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27265
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17373"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3532)
                     /locus_tag="VFI55_11005"
     CDS             complement(<1..3532)
                     /locus_tag="VFI55_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7074594.1"
                     /translation="MSSPSPSPKALARFSEITRDWFNSTFAAPTAAQAQAWAAIAEGR
                     NTLVIAPTGSGKTLAAFLWALDTLAHREAIPSGPDRPGGTRVLYVSPLKALAVDVERN
                     LRTPLAGLTRLAERQGLPPPNISVGVRSGDTPPATRRQLIAQPPDVLITTPESLFLML
                     TSAARETLAGVQTVIVDEIHAIAGGKRGAHLALSLERLDDLRDEPPAQRIGLSATVRP
                     PEELARFLSGPAPRTPTKIVAPPSAKTVELSVQVPVPDMANLDNNTIWPDVEARLVDL
                     IESHNSTIVFANSRRLAERLTARLNEIHAERSGVQLSSDTNPRVGGGAPAHVMASGQT
                     FGAPTLLARAHHGSVSKEQRALVEEDLKRGLLKAVVATSSLELGIDMGAVDLVIQVEA
                     PPSVASGLQRIGRAGHQVGEVSQGVLFPKHRTDLIGCAVSVQRMLAGQIESLRVPANP
                     LDILAQHTVAAAALEPLDADRWFDTVRRSAPFATLPRSVYEATLDLLSGKYPSTEFAE
                     LRPRLVYDRDTGTLTARPGAQRLAVTSGGAIPDRGLFTVYLAGESEKPSRVGELDEEM
                     VYESRPGDVISLGATSWRITEITHDRVLVIPAPGQPARLPFWRGDAVGRPAELGAALG
                     ALTGELANLDREAFGTRCAAWGFNDYAADNLWGLLDEQRTAAGVVPTDTTLLVERFRD
                     ELGDWRVILHSPYGLRVHGPLALAVARRLRERYGIDEKPTASDDGIVVRLPDTGLSPG
                     EDAPPGAELFVFDADEIDPIVTAEVGGSALFASRFRECAARALLLPRRHPGRRSPLWH
                     QRQRAAQLLDVARKYPDFPVVLEAIRECLQDVYDVPALIELMTGIAQRKVRVLETETA
                     RPSPFAASLLFGYVGAFMYEGDTPLAERRAAALSLDSTLLAELLGRVELRELLDPDII
                     AATGRQLQHLSDDRAARDAEGVADLLRLLGPLTEAEVANRARVADGIDVSGWLEGLRA
                     ARRALTVSFAGRSWWVAVEDIGRLRDGVGAAVPQGVPVTFTAAVADPLAELLGRYART
                     HTPFTTAEAAARFGLGLRVTADVLGRLAGPATSGGRLVRGDFVAPEVSPGGAGGAGGA
                     GGEQWCDADVLRILRRRSLAALRAQVEPVSTAAYGRFLPAWHRMAPSGQSGQAGLDGL
                     MSAIDQLAGVRIPASALEPLVLAPRVRDYSP"
     gene            complement(3566..4228)
                     /locus_tag="VFI55_11010"
     CDS             complement(3566..4228)
                     /locus_tag="VFI55_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7074595.1"
                     /translation="MPTARRRLSPEDRRAELLALGAEVFGKRPYDEVRIDEIAERAGV
                     SRALMYHYFPDKRAFFAAVVTDEADRLYEATNQLRPAGMTMFEEIRLGVLAYMAYQQQ
                     HPESAWAAYVGLGRSDPVLLGVSDEAKNRQMEHIMGRIAELANTVPGTGLEAEVERHI
                     RVIVHGWLALTFEICRQRIMDPTTDADRLADACAHALLDAISRVPNIPDGLAEAMATA
                     RL"
     gene            complement(4419..5120)
                     /locus_tag="VFI55_11015"
     CDS             complement(4419..5120)
                     /locus_tag="VFI55_11015"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7074596.1"
                     /translation="MDLSVRSTQPEVLDDENVDIADYQRCLAELAVINRVTFTHRPAL
                     RWLAHVTKALPVGETFSVLDVGYGDGDLLRAIARWADRRGLKPRLSGIDLNPRSAVAA
                     RSATPPNMSIEYRTDDVFSYTPDRPFDFIVSSQFTHHLSDADVVEFLIWIETNSVHGW
                     HIADLHRHPLAYYTFPILAGLMGWHQIVRSDGIISVARSFRRSDWQAYLEKANLQATI
                     SWYAFRFCVSRIDER"
     gene            complement(5398..6912)
                     /locus_tag="VFI55_11020"
     CDS             complement(5398..6912)
                     /locus_tag="VFI55_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7074597.1"
                     /translation="MNADDLRARARHALQATGVDVDLGEPGAPGLPASTPITGEVLFT
                     VPPTTVQQADDAIADAAQAFSTWRCTPAPVRGALVARLGELLTEHKSDVATLVTLEVG
                     KITSEALGEVQEMIDVCQFAVGLSRQLYGRTIASERPGHRLLETWHPLGVVGVITAFN
                     FPAAVWAWNAAVALVCGDTVVWKPSELTPLTALACQALVRRAAADVGARPALSGLVLG
                     DRELGERLVDDPRIALLSATGSVRMGQVVGPRVAGRFGRALLELGGNNAAIVTPSADL
                     ELAVRAIVFAAAGTAGQRCTSLRRLIVHQSVADVVVERVAAAFRQLPIGDPSAAGTLV
                     GPLIHETAYRDMVGALEQARADGGEVIGGDRHHRPGHPGAYYVAPAVVRMPAQTAIVA
                     AETFAPILYVLTYDDLDEAIALNNGVPQGLSSAIFTTDLREAERFLDGSDCGIANVNI
                     GTSGAEIGGAFGGEKQTGGGRESGSDAWQAYMRRATNTVNYSGELPLAQGVEFG"
     gene            complement(7024..8307)
                     /locus_tag="VFI55_11025"
     CDS             complement(7024..8307)
                     /locus_tag="VFI55_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1338 family protein"
                     /protein_id="HET7074598.1"
                     /translation="MKRQKRMATWQLRAQFAAGLSAMYAGEVPAYATLVEVSGQVNAG
                     YVAAHQGAVGLGSLQRVTAERHGAIRVGSPAELAAVADLFAAFGMFPVGYYDLRSARS
                     PIPVVSTAFRPIAAKELALNPFRVFTSMLATRDRRYFTHDLRARVETFLARRRLFDPA
                     LLARARAIAAEGGCDVEEADAFVAAAVSAFALSREPINKSWYDELSRVSPVAADIAGV
                     CSTHINHLTPRVLDIDELYRRMTERGITMIDAIQGPPRTDGPAVLLRQTSFRALDEPC
                     LFRGRDGTVRSGHVRVRFGEVESRGVALTRRGRERYDAAMANLAGEADPAKGWSRYFP
                     STDKEMAADGLAYYRGGDPSAPIVYEDFLPASAAGIFRSNLDRDTHTGEGADDSGYDA
                     AWLAGAIGRDIYDPYALYEALSQVGAPPACGGQSR"
     gene            8347..8799
                     /locus_tag="VFI55_11030"
     CDS             8347..8799
                     /locus_tag="VFI55_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET7074599.1"
                     /translation="MGETLDDIDRILMRELVADGRATLAELAASAGLSVSAVQSRVRR
                     LESRGVVAGYSARINPEAVGQLLSAFVAITPLDPSQPDDAPARLAHIEEIESCHSVAG
                     EESYILLVRVESPRALEDLLQRIRTAANVQTRSTIILNTFYSDRQYIP"
     gene            8853..10202
                     /gene="lat"
                     /locus_tag="VFI55_11035"
     CDS             8853..10202
                     /gene="lat"
                     /locus_tag="VFI55_11035"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HET7074600.1"
                     /translation="MTAAVNFCALPGSQTKPDRVHGVLSRSLLVDGLDLVLDLARSAG
                     SYLVDARDGRRYLDMFTFVGSSALGMNHPALTDDEAFLDELVEAALNKPSNPDVSTVA
                     MAGFVETFVRVLGDPALPHLFFVEGGALAVENALKVAFDWKSRHNQAHGLDPALGTRV
                     LHLRGAFHGRSGYTLSLTNTKPVSVARFPKFDWPRIDAPFIRPGLDGAAMDAREAESL
                     RQARAAFEAHPHDIACFLAEPIQGEGGDRHFRPEFFTAMRELCDQYDALLIFDEVQTG
                     CGLTGSPWAYQQLGVQPDVVAFGKKTQVCGVMAGRRVDEVADNVFAVSSRLNSTWGGN
                     LTDMVRARRILEVIEGDELFDQAAEQGRYLLARLDQLAREFPTVVLDPRGRGLMCAFS
                     LPTTADRDEVIRQVWQRGVIVLPSGTDGVRFRPALTVSRAEIDVAISAVRGALAAVA"
     gene            10371..10754
                     /locus_tag="VFI55_11040"
     CDS             10371..10754
                     /locus_tag="VFI55_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074601.1"
                     /translation="MQKADERSSDDRLGQEDGEVRAAIRFAVLSALAGVGFEVMAALW
                     VSTCPGTGVDTVACGPPQRTLLALGGPLILFAAGLWAFLRTYRVWKARGTWWGWHGAG
                     WFLLTLMVVALGLGVAPIAGPVLAL"
     gene            10852..11232
                     /locus_tag="VFI55_11045"
     CDS             10852..11232
                     /locus_tag="VFI55_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074602.1"
                     /translation="MRQILLRAVVLLGSWAIGLLVAAWVVPRVSLSASGFIVAVLVFS
                     VAQAVLSLSILKLPHGYASLLLGGTGLALTVVALILASELTHGLTIHGFASWLATTVL
                     VWLVTTVGAISLPDLFAREGAAAT"
     gene            11483..11794
                     /gene="usfY"
                     /locus_tag="VFI55_11050"
     CDS             11483..11794
                     /gene="usfY"
                     /locus_tag="VFI55_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein UsfY"
                     /protein_id="HET7074603.1"
                     /translation="MGDTHHDPTDYSRTTQPHAGIVMKDNFFWPGLILLVVALFGMIS
                     TAVAAGYHHYEWLTTTVLIAVLASVAGALWFLVEHRRVARIEEQWYTAHPDSRPRQHT
                     A"
     gene            12266..12697
                     /locus_tag="VFI55_11055"
     CDS             12266..12697
                     /locus_tag="VFI55_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7074604.1"
                     /translation="MTDAVLSSNKRQRGHRAVEVHVAARLENLAMLRTLVGAIGTFED
                     LDFDAVADLRLAVDEVCTRLIRSATPGATLVVVVDPQDDKLVVEASAACDTHDVVAPG
                     SFSWHVLTSLADDVQTFHDGREPDETGSVFGITLTARRAGL"
     gene            12701..13492
                     /locus_tag="VFI55_11060"
     CDS             12701..13492
                     /locus_tag="VFI55_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigF"
                     /protein_id="HET7074605.1"
                     /translation="MTARAGGGSVSRPNEYADVLEMFRELATVAPDSMEFQRQRDKIV
                     ERCLPLADHIARRFEGRGEPRDDLVQVARVGLVNAVVRFDVEAGSDFVSFAVPTIMGE
                     VRRHFRDNSWSVKVPRRLKELHLRLGAATADLSQRLGRAPTATELASELGMERDEVVE
                     GLVAGSSYNTLSIDTGGGSSDDDDSRAIADTLGDVDTGLDRIEDRESLRPLLEALPER
                     ERMVLVLRFFESMTQTQIAERVGISQMHVSRLLAKSLARLRDQLQ"
     gene            complement(13463..13882)
                     /locus_tag="VFI55_11065"
     CDS             complement(13463..13882)
                     /locus_tag="VFI55_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7074606.1"
                     /translation="MSVAQSWQRSRTAQFSARSSSLGTVITAQGELDAANADQLAAYV
                     QRSIKHSGRVVLDLRGLDFIGTAGFSALHRINVVCSAVQARWAMTPSPAVSRLLRVCD
                     PDGTLPVTTPRAEPLLQPTGARAQRSLTLLQLVAQPR"
     gene            complement(14103..15905)
                     /locus_tag="VFI55_11070"
     CDS             complement(14103..15905)
                     /locus_tag="VFI55_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HET7074607.1"
                     /translation="MASHASSRLARISKVLVANRGEIAVRVIRAARDAGLASVAVYAE
                     PDADAPHVRLADEAFALGGQTSAESYLDFGKILDAAAKSGANAIHPGYGFLSENADFA
                     QAVLDAGLIWIGPSPQSIRDLGDKVTARHIAARAQAPLVPGTPDPVKDADEVVAFAKE
                     YGVPIAIKAAFGGGGRGMKVARSIEEIPELFESATREAVAAFGRGECFVERYLDKPRH
                     VEAQVIADQHGNVVVAGTRDCSLQRRFQKLVEEAPAPFLTDAQRKEIHESAKRICKEA
                     HYYGAGTVEYLVGQDGLISFLEVNTRLQVEHPVTEETAGIDLVLQQFKIANGEKLDIT
                     EDPTPRGHAIEFRINGEDAGRGFLPAPGPVTRYDIPTGPGVRLDSGVEAGSVIGGQFD
                     SMLSKLIVYGANRPEALGRARRALAEFHVEGLATVIPFHRAVVSDPAFIGDDSGFSVH
                     TRWIETEWNNTVEPFTGGEPLDDDDARPRQKVVVEVGGRRLEVSLPGDLALSNGAADP
                     AGVIRKKPKARKRGAHSGAVASGDAVTAPMQGTVVKVAVEEGQEVAVGDLVVVLEAMK
                     MENPVTAHKDGVITGLAVEAGAAITQGTVLAEIK"
     gene            complement(16004..16435)
                     /locus_tag="VFI55_11075"
     CDS             complement(16004..16435)
                     /locus_tag="VFI55_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HET7074608.1"
                     /translation="MSMPAPLAEVVSDFAEVQGQDKLTLLLEFANELPALPSDLVEAA
                     MEPVPECQSPLFLHVDASDPERVRLHFSAPAEAPTTRGFASILAAGLDEQPAADILAV
                     PEDFYTDLGLATLISPLRLRGMSAMLARIKRHLRETSPATI"
     gene            complement(16432..17325)
                     /locus_tag="VFI55_11080"
     CDS             complement(16432..17325)
                     /locus_tag="VFI55_11080"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HET7074609.1"
                     /translation="MSLPPDPSPSLSVYSHPERLVTADWLSAKMGTPGLVIVESDEDV
                     LLYDVGHIPGAVKVDWHTDLNDPRVRDYINGAQFAELMNRKGIARDDTVVIYGDKSNW
                     WAAYALWVFTLFGHPDVRLLNGGRDLWLAERRETTLEVPTKTSTGYPVVQRNDEPIRA
                     FKDDVLGILGSQPLIDVRSPEEYTGKRTHMPDYPEEGALRAGHIPTARSIPWGKAVDD
                     SGRFRNREELERLYDFINPDDKTVVYCRIGERSSHTWFVLTHLLGKPGVRNYDGSWTE
                     WGNAVRVPIVAGEDPGAVPAR"
     gene            complement(17361..18002)
                     /locus_tag="VFI55_11085"
     CDS             complement(17361..18002)
                     /locus_tag="VFI55_11085"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HET7074610.1"
                     /translation="MTRMVLGSASPGRLKVLRQAGIDPLVMVSGVDEDVVTAGLGPDA
                     SPSEVVCALAAAKAEQVAGKLDRAVAADCVVIGCDSMLYIDGRLCGKPHTAGAARQLW
                     RSMSGRAGQLYTGHCVIRLLDNETVHRDSAASTTTVHFGTPSPDDLEAYLASGESLRV
                     AGGFTLDGLGGWFIDGVDGDPSAVVGIGLPLTRSLLARAGLPIAALWADNPLA"
     gene            complement(17999..18235)
                     /locus_tag="VFI55_11090"
     CDS             complement(17999..18235)
                     /locus_tag="VFI55_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit epsilon"
                     /protein_id="HET7074611.1"
                     /translation="MTDTTAPQHPHEPHIQILKGQPTDEELAALIVVLGSAGRGPEPG
                     QPERTRWGLPVDRLRYAISNYQRITLQERMHMQR"
     gene            complement(18250..19890)
                     /locus_tag="VFI55_11095"
     CDS             complement(18250..19890)
                     /locus_tag="VFI55_11095"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HET7074612.1"
                     /translation="MTSVTGHTAEPAAEHTIDIHTTAGKLAELHKRRAEALHPVGEAA
                     IEKVHAKGKLTARERIYALLDDDSFVELDALARHRSTNFGLAENRPLGDGVVTGYGTI
                     DGRDVCIFSQDVTVFGGSLGEVYGDKIVKVQELAIKTGRPLIGINDGAGARIQEGVVS
                     LGLYSRIFRNNIVASGVIPQISLIMGAAAGGHVYSPALTDFVVMVDQTSQMFITGPDV
                     IKTVTGEDVTMEELGGAHTHMAKSGTAHYVASGEQDAFDWVRDLLSYLPPNNFTDPPR
                     YAEPIPEGAIEDNLTEEDLELDTLMPDLPNQPYDMHEVITRILDEDEFLEVQAGYAQN
                     IVVGFGRIEGRPVGIVANQPTHFAGCLDINASEKAARFVRTCDCFNIPIVMLVDVPGF
                     LPGTGQEYNGIIRRGAKLLFAYGEATVPKITVITRKAYGGAYCVMGSKDMGCDVNLAW
                     PTAQIAVMGASGAVGFVYRQQLAEAANQGQDVDTLRLQLQEEYEDTLVNPYVAAERGY
                     VDAVIPPSHTRGYIGTALRLLERKISHLPPKKHGNIPL"
     gene            19956..20783
                     /locus_tag="VFI55_11100"
     CDS             19956..20783
                     /locus_tag="VFI55_11100"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015303798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HET7074613.1"
                     /translation="MTDRELFRQPLDAAALRAELIGTGLGWRQLDVVERTGSTNADLL
                     ARAASGTDVAGTVLIAEHQTAGRGRHGRAWSASPRAQITMSVGVSVVDVPAEGWGWLS
                     LATGVAIVDALAPVLAAIGIQAGLKWPNDVLAGPAGAPGKLAGILAEVARPVVVIGVG
                     LNVTQAPEEVDGPGATSLLDLGVAAPDRNQLACALLRELGRRIVAWRAARGADWALAA
                     DYRARSLTIGTRVRAYLPGDKELVGTASGVDDHGRLCLETASGQTAVVSAGDVVHLR"
     gene            20822..21340
                     /locus_tag="VFI55_11105"
     CDS             20822..21340
                     /locus_tag="VFI55_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET7074614.1"
                     /translation="MSYPDNALAPGEQVVVHRHPHWKRLIWPVLVLILVTGLAAFGSG
                     YVNSTHWQQLAKNIVYGVIWGIWLIIVGWLTLWPFVSWLTTHFVVTSRRVMFRHGVLT
                     RSGIDIPLARINSVEFRDRLFERMFRTGTLIIESASQDPLEFYDIPRLREVHSLLYHE
                     VFDTLGSEESPS"
     gene            complement(21295..21984)
                     /locus_tag="VFI55_11110"
     CDS             complement(21295..21984)
                     /locus_tag="VFI55_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET7074615.1"
                     /translation="MSFADATIARLPQAVQPYAQRHHELIKFAIVGGTTFVIDSVIFY
                     TLKLTILETKPVTAKVIAGIVAVIASYVLNREWSFRDRGGRERHHEALLFFAFSGVGV
                     LLSMAPLWVSSYVLQLRVPMVSLTLENIADFISAYIIGNLLQMAFRFWAFRRWVFPEE
                     FARSPDKALESALTAGGIAEVFEDAFEGGFEEGLQGLEGNVTLLRSWRNRASRLAQLG
                     DSSEPRVSKTS"
     gene            22095..23357
                     /locus_tag="VFI55_11115"
     CDS             22095..23357
                     /locus_tag="VFI55_11115"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET7074616.1"
                     /translation="MMAVPSRRPPEVAPLVAMVGGGQLARMTHQAAIALGQTLRVLAT
                     STDEPAAQVTPDVVIGSHTDLEDLRRVAVGADVLTFDHEHVPNELLVKLVAEGVNVAP
                     PPQALVHAQDKLVMRRRLQALGVPVPRYAEIRSMDELDAFARRIAGPVVVKAVRGGYD
                     GRGVRMARDPSHAREIAAAFLADGVPVMAEEQVNLRRELSALVARSPFGQGAAWPVVE
                     TVQRDGICVQVIAPAPDLPDDLASAAQQLALRLAGELGVVGVLAVELFETTDRALLVN
                     ELAMRPHNSGHWTMDGSRTSQFEQHLRAVLDYPLGDTEVIAPVTVMANVLGAARQPAM
                     ALDERLHHLFARMPDARVHLYGKAERAGRKIGHINFVGSNATDLGMLRDRAELAAHWL
                     SHGQWTDGWDSHRAGDDAERSDEEERRP"
     gene            23392..23925
                     /gene="purE"
                     /locus_tag="VFI55_11120"
     CDS             23392..23925
                     /gene="purE"
                     /locus_tag="VFI55_11120"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET7074617.1"
                     /translation="MTQQSSGAERPRVGVIMGSDSDWPVMIDAAEALAEFDIPIEVRV
                     VSAHRTPDVMLDYARGAADRGLEVIIAGAGGAAHLPGMVASATPLPVIGVPVPLARLD
                     GLDSLLSIVQMPAGVPVATVSIGGARNAGLLAVRILASSDPQLRARIAAFQRRLADSV
                     RAKDAALQQLHDKVSDE"
     gene            23940..25109
                     /locus_tag="VFI55_11125"
     CDS             23940..25109
                     /locus_tag="VFI55_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET7074618.1"
                     /translation="MAGWAGNPSFDLFQLPEEHQEFRAAIRALAEKEIAPYANDVDEQ
                     ARFPEEALAALNASGFNAVHVPEEYGGQGADSVAACIVIEEVARVDASASLIPAVNKL
                     GTMGLILRGSEELKKQVLPTLATEGAMASYALSEREAGSDAASMRTRAKADGDDWILN
                     GAKSWITNGGKSTWYTVMAVTDPDKGANGISAFIVHKDDEGFSVGPKEKKLGIKGSPT
                     TELYFENCRIPGDRIIGEPGTGFKTALATLDHTRPTIGAQAVGIAQGAVDAAIAYTKD
                     RKQFGESISTFQAVQFMLADMAMKVEAARLMVYSAAARAERGEPDLGFISAASKCFAS
                     DVAMEVTVDAVQLFGGAGYTIDFPVERFMRDAKITQIYEGTNQIQRVVMSRALLR"
     gene            25360..26550
                     /locus_tag="VFI55_11130"
     CDS             25360..26550
                     /locus_tag="VFI55_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HET7074619.1"
                     /translation="MPVRAPVIPVAHWQSLSLLLVEDDRADAVLVEDVIADAVADIRV
                     IWAQSMAHAERELASARPDCVLLDLNLPDANGIDALDRIAKRDATVPIVVLTGLNDEY
                     FGASAVAAGAQDYLVKGRVEPEMLRRALLYAIERKRAELISADLRASRQRARENALLE
                     RGLLPSPLLLDDPGVDIVARYRPSREDALLCGDFYDVVQTPDRVVHVLIGDVAGHGPH
                     EAALGAALRIAFRALTLAGVQGVELMRLLERVLHSERTATGVFATVLSLEISPDCPRI
                     TTVRAGHPGMLRQNDGGVEWIEPPGGPALGLRADDWPSHELDLPAGQGLLLLTDGLFE
                     GYSGRGNKRLGEDGLLALARSHARLPGPAFVDALIDGAQELAQPRGGLTDDIAVLRVE
                     RTTT"
     gene            26622..>27265
                     /locus_tag="VFI55_11135"
     CDS             26622..>27265
                     /locus_tag="VFI55_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HET7074620.1"
                     /translation="MGVMVLAGALVGAVLLYRTDETSRELTDDIQPARLAAYQLQSAL
                     RDQETGVRGYVIAADRQFLTPYYDGQHAEREAAEDVRRHVGQRADLVGDLDAIEKAAA
                     NWRTNYAEPLIASVAPNGRNVANLPAAERGKVEFDHLRALFDTQNANLTAAWAHAADD
                     LKDTDAWRDRVLGAMVVLFFGTAALLGLLQRDVVTRPLAALAASCRRITEGNFG"
BASE COUNT         4384 a         8918 c         9360 g         4603 t
ORIGIN      
        1 ccggggagta atcgcggacc cgtggggcca acaccagagg ttcgagcgcc gaggccggaa
       61 ttcgaacgcc ggcaagctga tcgatcgcgg acatcagtcc gtcaaggccc gcctgaccgg
      121 actggccaga cggggccatt cggtgccagg ccggcaggaa tcgcccatac gcggcggtgc
      181 tcaccggctc cacctgggcg cgcaacgctg ccagcgaacg gcgccgcagg atgcgcaata
      241 cgtcggcgtc gcaccactgt tcgccgcccg ccccgcccgc cccgcccgcc ccgccagggg
      301 agacttcggg agcgacgaag tcaccgcgaa ccagcctgcc tcccgaagtt gccgggccgg
      361 ccagtcggcc cagcacgtcc gcggtcaccc gtagtcccag gccgaagcgg gcggcggctt
      421 cggcggtggt gaacggggtg tgcgtgcgcg cgtagcggcc cagcagctcg gccagcgggt
      481 cggccaccgc tgcggtgaag gtcaccggga cgccctgtgg aaccgcggcg ccaaccccgt
      541 cgcgcaaccg gccgatgtct tcgacggcca cccaccagct gcggccggcg aacgacaccg
      601 tcagcgcgcg cctggcggcg cgcaagcctt ccagccagcc gctgacgtcg atgccgtcgg
      661 cgaccctggc cctgttggcg acttcggcct cggtcaacgg gcccagcagt cgcagcagat
      721 cggcgacccc ttcggcgtcg cgggcggctc ggtcgtccga caggtgctgt aactggcgcc
      781 cggtcgcggc gatgatgtcg ggatcgagca gctcacgcag ctccactcgg ccgagtagct
      841 cggccagcag cgtgctgtcc agcgagagcg ccgcggcgcg gcgctcggcc agcggcgtat
      901 cgccctcgta catgaacgcg ccgacgtaac cgaacagcag cgaggccgcg aacggggagg
      961 gcctggccgt ctcggtctcc agcacccgca ccttgcgctg ggcgatgccg gtcatcagct
     1021 cgatcagcgc gggaacatcg tagacgtcct gcaggcactc gcggatggcc tccagtacca
     1081 ccgggaagtc ggggtacttg cgcgccacgt ccagcagttg ggccgcgcgc tggcgctggt
     1141 gccacagggg cgagcggcgg ccggggtggc ggcggggcaa cagcagcgcg cgggcggcgc
     1201 actctcggaa ccgcgacgcg aacagcgccg aaccacccac ttcggctgtg acgatcgggt
     1261 cgatctcgtc ggcgtcaaag acgaacaact cggcacccgg tggggcgtcc tccccgggag
     1321 acaaaccagt gtcgggcaat cgcaccacga tgccgtcgtc ggaagcggtc ggcttttcgt
     1381 cgatcccgta gcgctcgcgc agccgccggg ccactgccag cgccagcggg ccgtgcaccc
     1441 gcagcccgta cggtgaatgc aggatcaccc gccagtcgcc gagttcgtcg cggaaccgct
     1501 cgaccagcag ggtggtgtcg gtgggtacca ctccggcggc ggtgcgctgc tcgtccagca
     1561 gcccccacag gttgtcggcc gcgtagtcgt tgaaacccca agccgcacaa cgtgtgccga
     1621 aagcctcgcg atccaggttg gcgagctcgc cggtgagcgc gccgagtgcg gcccccagtt
     1681 cggccgggcg gccgacagcg tctccgcgcc agaacggcaa ccgcgccggc tggccgggcg
     1741 ctggaatcac cagcacccga tcgtgggtga tctcggtgat ccgccagctg gtggccccca
     1801 gcgagatcac atcaccaggg cgagactcat acaccatttc ttcgtcgagt tcgcctaccc
     1861 gcgaaggctt ttcagactcg ccagcgaggt agacggtgaa cagcccgcga tcgggaatgg
     1921 cgccgccgga ggtgacggcc agccgttgcg cgccgggccg cgcggtgagc gtgcccgtgt
     1981 cgcggtcgta gaccaaccgc ggtcgcagct cggcgaactc ggtggacgga tacttgcccg
     2041 acagcaggtc cagcgtggcc tcgtagacgc tgcgcggcag cgtcgcgaaa ggcgcgctgc
     2101 gccgcacggt gtcgaaccag cggtcggcgt ccagtggctc gagcgcggca gcggccacgg
     2161 tgtgttgggc caggatgtcg agtgggttgg cgggcacccg cagcgactcg atctggcccg
     2221 cgagcatccg ttgcacgctg accgcgcagc cgatcaggtc ggtgcggtgc ttggggaaca
     2281 gcaccccctg tgaaacctcg cccacctgat gtccggcccg cccgatgcgc tgcagcccgc
     2341 tggccaccga cggcggcgcc tccacctgga tcaccagatc gaccgcgccc atgtcgatgc
     2401 ccagctccag gctcgacgtc gcgacgaccg ccttgagtag cccgcgcttg aggtcctctt
     2461 cgaccagggc acgctgctct ttgctgaccg aaccgtgatg ggcgcgggcc agcagcgtgg
     2521 gcgcgccgaa ggtctggccg ctggccatga cgtgcgccgg cgccccaccg ccgacccgcg
     2581 ggttggtgtc cgacgacagc tggacgccgg agcgttcggc gtggatttcg ttgagccgtg
     2641 cggtgagtcg ctccgccaac cgtcgcgaat tggcaaacac gatggtcgag ttgtgcgatt
     2701 cgatcaggtc gaccagccgc gcctcgacgt cgggccagat ggtgttgttg tccaggttgg
     2761 ccatgtcggg caccggcact tgaacgctga gttcgacggt cttggccgac gggggagcca
     2821 cgatctttgt cggagtcctc ggcgccggcc cggacaggaa ccgtgccagc tcctcggggg
     2881 gacgcacggt cgccgacagt ccgatccgct gcgcgggcgg ttcgtcgcgc agatcgtcca
     2941 gccgctccag cgacagcgca aggtgcgcgc cgcgcttgcc gccggcgatg gcgtggatct
     3001 cgtcgacgat caccgtctgc actccggcca gcgtttcgcg cgcggccgac gtcagcatca
     3061 agaacaatga ctccggggtg gtgatcagca catcgggcgg ctgcgcgatg agctggcggc
     3121 gggtcgccgg cggggtgtcg ccggaacgca cgccgacgct gatgttgggt ggcggcaggc
     3181 cttgacgctc ggcgagccgg gtcagccccg ccaacggggt gcgcaggttg cgctcgacgt
     3241 cgaccgccag cgccttgagc ggtgacacgt acagcacgcg ggtgcctccg ggccgatccg
     3301 gtccggacgg gatagcctcc cgatgggcca gggtgtccag cgcccacagg aacgccgcca
     3361 gcgtcttgcc ggatccggtc ggggcgatca ccagagtgtt ccggccctcg gcgatggcgg
     3421 cccaggcttg tgcctgtgca gcggtcggtg ccgcgaaggt gctgttgaac cagtcgcggg
     3481 tgatctcgct gaaccgggct aacgccttcg gcgacgggct agggctgctc acctagaaca
     3541 tcgtgccaag ggataccgac acaactcaca gtcgcgcggt ggccatggcc tcggccaggc
     3601 cgtccgggat gttggggacc cgactgattg cgtccagcag cgcgtgcgcg cacgcgtcgg
     3661 ccagccggtc ggcgtcggtc gtggggtcca tgatccgctg acggcagatc tcgaaggtga
     3721 gcgcgagcca gccatggacg atgacccgga tgtgccgctc gacctcggct tccaggcccg
     3781 ttcccgggac ggtgttcgcc agttcggcga tccggcccat gatgtgttcc atctggcggt
     3841 tcttggcttc gtcgctcacg ccgagcagca ctgggtcgga ccggcccaga ccgacgtacg
     3901 ccgcccacgc ggactccggg tgctgttgtt ggtaggccat ataggcgagc acaccgagtc
     3961 tgatctcttc gaacatcgtc atgccggcag ggcgaagctg gttggtggcc tcgtagaggc
     4021 gatccgcctc atcggtgacg accgcggcga agaacgcccg tttgtcaggg aaatagtgat
     4081 acatcagggc gcgggacact ccggcgcgct cggcgatttc gtcgatgcgg acctcgtcgt
     4141 acggccgctt cccaaagact tccgccccca gagcaagcag ctcagctcgg cgatcctccg
     4201 gggacaatcg ccttctagct gttggcatgt gtcagatagt acccacggag gcggagtcgt
     4261 aattcctact gtgattatct accggcgtgg gctcctcgcg gcgctcagtc catgaacccg
     4321 acgacaccag cgcggacaga gtcgcttcca aatggatcac gtcggatgaa agccgatttc
     4381 cacgcatacg ttatgcgacc cgaaacgatt gcgaatcctt atcgttcatc gatccgggac
     4441 acgcagaatc ggaacgcgta ccaggaaatt gttgcctgca agttcgcctt ttccaggtac
     4501 gcttgccaat cgctacggcg aaaacttcgg gcgacggaga ttatgccgtc agagcgcacg
     4561 atttggtgcc aacccatcag cccggccaga attggaaagg tgtaatacgc caacggatgg
     4621 cgatggagat ccgcaatgtg ccagccgtgt acggaattcg tctcgatcca gatgaggaac
     4681 tcgacaacgt ccgcgtcact gaggtgatgc gtgaactgcg agctgacgat gaaatcgaag
     4741 ggcctgtccg gggtgtaaga aaatacgtcg tccgttcgat actcgatgct catattcggt
     4801 ggcgttgcgc tgcgcgccgc gacagcgctg cgggggttca gatcgatgcc tgacaatcgg
     4861 ggctttagcc cgcgccgatc tgcccagcgt gcgatggcgc gcaacaaatc gccgtcacca
     4921 tatccgacgt ccagcacgga gaatgtctca ccgacgggca atgcctttgt tacatgcgcc
     4981 agccagcgca atgcggggcg gtgcgtgaag gtgacgcgat tgatgacggc aagttctgcc
     5041 aagcaccgct gatagtcggc tatgtcgaca ttttcgtcat ccaacacttc cggctgcgtg
     5101 ctgcgaacag agagatccat tcgtcctccg tcacgtcgga cctcccgatc cgtgtttcgg
     5161 tagtccccca cgaggtggtc ccggcgctcc cgtgctcccc cgatggcgtt gggaccccat
     5221 tttcgcctct agcgtagggc aaatcggggg tccccgaggg ccgacgagga gtgcgcatcc
     5281 ggccgagctc gaccggtgtc gagcggcaga cgatttggct gcgtggttac cgcccgtaag
     5341 gggtgggagt cgccgagccg aggcgacgac gcgcgctgtt gcttggcttt gcttcggtta
     5401 tccgaactcc acgccctggg ccagcggtag ctcgccggaa tagttcaccg tgttagtggc
     5461 gcgccgcatg taagcctgcc acgcgtccga gccggattca cgtccgcctc cagtctgctt
     5521 ttcaccgccg aacgccccgc cgatctccgc accggatgtg ccgatattga cgttggcgat
     5581 gccgcaatcg gatccgtcca ggaaacgctc ggcctcccgc aggtcggtgg tgaaaatggc
     5641 cgatgaaagc ccctgcggca ccccattgtt gagcgctatc gcctcatcca ggtcgtcgta
     5701 ggtcagtaca tagaggatcg gcgcgaacgt ttcggcagcc acgatggcgg tctgcgcggg
     5761 catgcggacc acggccggcg cgacgtagta cgcacccgga tgtcccggtc ggtggtggcg
     5821 gtcgccgccg atgacctcgc cgccgtcggc gcgtgcctgc tccaaagctc cgaccatgtc
     5881 gcggtaggcg gtctcgtgga tgagcggccc gacgagcgtg cccgccgccg acgggtcgcc
     5941 gatcgggagc tgccggaaag cagccgccac ccgttccacg accacgtcgg ccaccgactg
     6001 atggacgatc agccggcgca ggctcgtgca gcgctggccg gcggtaccgg cggcggcgaa
     6061 cacgatggcg cgcacggcca gttccaggtc ggccgacggt gtcacgatgg ccgcgttgtt
     6121 gccgcccaat tccaagagcg cccgcccgaa gcgcccggcc acgcgtggac cgaccacttg
     6181 gcccattcgt accgaacccg tcgcggacag cagcgcgatc cgggggtcgt ccaccagtcg
     6241 ttcacccagc tcgcggtcgc ccaggaccag gccgctcagc gcgggccggg caccgacgtc
     6301 ggcggcggcc cgccggacca gcgcctggca ggccagggcc gtcaacggcg tcagctccga
     6361 gggcttccac accaccgtgt ccccgcacac cagggccacc gcggcgttcc acgcccagac
     6421 cgctgcaggg aaattgaacg ccgtgatgac gcccaccacg ccgagcggat gccaggtctc
     6481 cagcaaacga tgcccaggac gttccgatgc gatggtccgg ccgtagagct ggcgcgacaa
     6541 gccgacggcg aactggcaga catcgatcat ttcctgcacc tcgcccagcg cctcggaggt
     6601 gatcttgccc acctcgaggg tcaccagcgt tgcgacgtcg ctcttgtgtt cggtgagcag
     6661 ctcgccgagc cgggccacca gcgcgccgcg taccggtgcc ggtgtgcacc gccacgtcga
     6721 gaatgcctgc gccgcatcgg cgatggcgtc atcggcctgt tggaccgtgg tgggtgggac
     6781 ggtgaagagc acctcaccgg tgatcggtgt gctggcgggc agcccgggcg cgcccggctc
     6841 gcccaggtcg acgtcgaccc cggtcgcctg cagggcgtgg cgtgcccggg cgcgcaaatc
     6901 gtcggcgttc atgagcgcca ctccttcttt ctgttgccct ctgcatcgtc gccggcgtcg
     6961 gtcatcagcg ccgctccttc tcatcgctgc gctctgcatc gtcgccggcg cgggtcggcc
     7021 gcttcacctg gattgtcccc cgcaagcggg aggtgccccc acctgagaaa gcgcttcgta
     7081 gagtgcgtac gggtcgtaga tgtcgcggcc gatggccccg gcgagccagg ccgcgtcgta
     7141 accggaatcg tcggctccct caccagtgtg ggtatcacgg tccaggttgg agcggaagat
     7201 tcccgccgcc gaggcgggca ggaaatcttc gtagacgatc ggcgccgacg ggtcgccgcc
     7261 gcggtagtag gccagtccgt cggccgccat ctccttatcg gtcgaaggga agtagcggct
     7321 ccaccccttg gcggggtcgg cttccccagc aaggtttgcc atcgcggcgt cgtagcgctc
     7381 gcgcccgcgg cgggtcaggg ctacaccgcg cgactcgact tcgccgaacc gcacccgaac
     7441 gtgtcccgag cgcacggtgc cgtccctgcc gcgaaacagg cacggttcgt cgagtgcgcg
     7501 aaaagatgtc tgccgcaaca acaccgcggg accgtcggtg cggggcgggc cctggatggc
     7561 atcgatcatg gtgatgccgc gctcggtcat ccggcggtac agctcgtcga tgtcgaggac
     7621 tcgcggcgtc aggtggttga tgtgcgtgga gcagacccct gcgatgtcgg cggccactgg
     7681 cgatacccgg gacaactcgt cgtaccagga cttgttgatc ggctcgcgcg acagcgcgaa
     7741 tgccgacacc gccgccgcga caaaggcatc cgcctcctcg acatcgcagc caccctcggc
     7801 ggcgatcgcc cgggcccggg cgagcaatgc ggggtcgaac agccggcggc gcgcgaggaa
     7861 ggtctccacc cgggcgcgca ggtcgtgagt gaaataccgc cgatcccggg tcgccagcat
     7921 cgaagtgaac acccggaatg gattgagagc cagctccttt gccgcgatcg gccgaaacgc
     7981 cgtcgacacc acgggaatgg gcgagcgggc cgagcgcagg tcgtagtagc ccaccggaaa
     8041 catgccgaac gcggcgaaca ggtcggcaac ggcggccagt tccgccggac tgcccacccg
     8101 gatggcgcca tgacgctcgg cggtgaccct ttgcagcgag cccagcccta cggcaccttg
     8161 atgggctgcc acgtaaccgg cgttgacttg cccgctcacc tcgaccagcg tggcgtacgc
     8221 gggcacctcg ccggcgtaca tggccgagag gccggcagcg aattgcgcac gcagctgcca
     8281 ggttgccatc cttttctgtc gtttcagcgg gctcatgccc agccgtcgct gcgatagtct
     8341 ccgcccatgg gtgagacgct cgacgatatc gaccggatcc tgatgcgcga gctggtcgcc
     8401 gacgggcgcg ccacgttggc ggagctggcc gcgagcgccg ggctgtcggt gtcggcggtc
     8461 cagtcgcggg tgcgtcggtt ggagtctcgc ggcgtggtgg cgggatactc agcgcggatc
     8521 aacccggagg ccgtcgggca gctgctgtcg gcgttcgtgg ccatcactcc tctggatcct
     8581 tctcaacccg atgatgcgcc cgcccgcctc gcacacatcg aagagatcga atcgtgtcat
     8641 tcggtggccg gcgaagagag ctacatcttg ctggtgcgtg tggagtcccc ccgggcgctg
     8701 gaggatctgc tgcaacggat ccggaccgcg gcgaacgttc agactcgaag caccatcatc
     8761 ctcaatactt tttacagtga tcggcaatac ataccatagt aattatggta ttagcgtcat
     8821 gattccgtaa aaactacgct agaatagacg gcatgaccgc cgccgtgaac ttctgtgcgc
     8881 tgcccggttc gcagacgaag cccgatcggg tccatggggt gctgtcgcgc agcctgctgg
     8941 tcgacggtct ggatttggtg ctcgatctgg cgcggtcggc cggctcgtat ctggtcgacg
     9001 cccgcgacgg tcggcgctat ctcgacatgt tcaccttcgt cgggtcgtcg gcgctgggca
     9061 tgaaccaccc ggcgctgacc gacgacgaag cgttcctcga cgaactcgtc gaggctgcgc
     9121 tgaacaagcc gagcaacccc gacgtgagca ccgtggcgat ggccggcttc gtcgagacct
     9181 ttgtccgggt cctgggtgac ccggcgctgc cgcacctgtt cttcgtcgag ggcggtgcac
     9241 tggcggtgga gaacgcgctc aaggtcgcgt tcgactggaa gagccggcac aaccaggcgc
     9301 acggcctcga tccggcgctt ggcacccggg tgctgcattt gcgcggcgcg ttccatggcc
     9361 gcagcggcta caccctgtcg ttgaccaaca ccaagccggt cagcgtggct cggttcccga
     9421 agttcgactg gccgcgcatc gatgcgccgt tcatccgccc gggcttggac ggtgcggcga
     9481 tggacgcgcg ggaagccgaa tcgctgcggc aggcccgggc ggcgttcgag gcgcatccgc
     9541 acgacatcgc ctgctttctg gccgagccca tccagggcga aggcggcgac cggcactttc
     9601 gccccgagtt cttcacggcg atgcgcgagc tgtgcgacca atacgacgcg ctgctgatct
     9661 tcgacgaggt gcagaccggt tgcgggctga ccggaagtcc ttgggcctac cagcaattgg
     9721 gtgtgcaacc cgatgtggtg gcgttcggta agaagacgca ggtatgcgga gtcatggcgg
     9781 gccgccgggt cgacgaggtc gccgacaacg tcttcgcggt ctcatctcgg ctcaactcga
     9841 cgtggggcgg caacctgacg gacatggtgc gcgcccgccg catcctggag gtgatcgagg
     9901 gcgacgaatt gttcgaccag gccgccgagc agggccggta cctcctggcc cggctcgacc
     9961 aactggcccg ggagttcccc acggtcgttc tcgacccgcg gggccgcggc ctgatgtgtg
    10021 cgttcagcct gccgactacc gccgatcgcg acgaggtgat ccgccaggtg tggcaacgtg
    10081 gcgtgattgt gctgccctcg ggcaccgacg gcgttcgctt ccgtcccgcg ctgaccgttt
    10141 cccgcgccga aatcgacgtg gcgatttctg ccgtgcgcgg tgctttggcg gctgtcgcgt
    10201 agctgcagaa aggcggttta tcgtacattg ccgcggggta tttgttccga tgcgatctcc
    10261 gttcgggagc cggcggcacg ggtcggtaaa agttcgcgct gaaacggtaa ccaatgcggc
    10321 gcagcgaacg atggactcag tacatctggg cgcaggtatg aggggcaggg atgcaaaagg
    10381 cggacgagcg gtcatccgat gatcgcttgg gacaggaaga cggcgaggtg cgcgccgcca
    10441 tccggtttgc cgtcctgtcc gccctcgctg gggtcggctt tgaggtgatg gccgcgttgt
    10501 gggtcagcac ctgccccgga accggcgtcg acacggtggc ctgcggcccg ccacagcgga
    10561 cgctgttggc gttgggcggt ccgctgatct tgtttgccgc cgggctgtgg gcgttcctgc
    10621 gcacctaccg agtgtggaag gcccggggta cctggtgggg atggcacggc gccggctggt
    10681 ttttgttgac gctgatggtg gtggcgctcg gtttgggtgt tgcgccgatt gcgggcccag
    10741 tcctggccct gtgagccacg agtgtggcga gcgcgaccag cggcgtcagt acatcgtgat
    10801 caaccaaagc ggatcttcac agccacactc ccgagaccta cgcttattgc catgaggcaa
    10861 atcctgctcc gcgcggttgt gcttcttgga tcgtgggcga tcggactact ggtcgcggcg
    10921 tgggttgttc cccgagtttc gttgtcggcg tcggggttca tcgtcgcggt actggtgttc
    10981 tcggtggcac aggcggtcct gtcgttgtcg atcttgaagt tgccccatgg ttatgcgtcg
    11041 ctgctcctcg gcggcaccgg gctggcattg actgtcgtcg cgttaattct cgcctcggaa
    11101 ttgactcacg ggcttaccat tcacggcttt gcatcctggc tggccacaac ggttttggtg
    11161 tggctcgtga cgacggttgg ggcaatttca ctgcctgacc tgttcgcccg cgagggcgct
    11221 gcagcgacct gaccaaatcg acgctgcgcg tgggagccgt gtattcgcga cgcggtgcaa
    11281 attcagccgg cctctaaagg gccgccaaag cgactcgcaa ccccaacaag aaagctccct
    11341 ttccggccgg cgggtgtctg gcgagttgtg cgcgccgtcg agtattcgcg ccggtcaccg
    11401 gcccgagtga attcggcatt tcgctgcgtc cgcctaatgg gtagcatttt tgttgtctca
    11461 tccgacgagg gagggagacg tgatgggaga cacgcatcac gatccgacgg attactcccg
    11521 gacgacgcaa cctcacgcgg gcatcgtcat gaaggacaat tttttctggc ccggcttgat
    11581 tctgttggtt gtggcactgt tcggaatgat cagcacggcg gtggcagccg gctaccacca
    11641 ctacgagtgg ctcaccacga cggtactgat cgccgtcctc gcatcggtcg ccggagcact
    11701 gtggttcctc gtggagcatc gccgtgtggc ccgcatcgaa gagcagtggt ataccgccca
    11761 tcccgatagt cggccccgcc agcacaccgc ctgacgcgaa gacgtactgc gaacgccgcg
    11821 tcgctacgct ggcatccgtc gtcgtgtcag catccgatca ccgccatgcc gtctgacggc
    11881 tcacctgcct gttggccgcg agatgtcatg gggaaatttg gtcggaaact caaaccgtgg
    11941 gttattggcc tcgtcgagtg ggtatcagct ctacaccatg atgagagccg gaaggattgc
    12001 gtcagcgtgg tcgccccatc gtcggggaca gaatcacgaa aagcatcgac atcaatgcct
    12061 actgccaaaa cgatcaatat gaccagcgtg atcccggcga tcaccgcgac gcgctggagt
    12121 cggcgcgtcc ggaggtcggt catgtcaggc ccgtccgtat cgggcggaat acccggcggc
    12181 catcatgact aaccggctgg aacgagcggt cgacgatggg ttgcgtggcg gtagtagtgt
    12241 cgatcagaca ttgccgggag accagatgac cgatgcggta ttgagctcca acaagcgtca
    12301 acgcggccac cgcgccgtcg aagtgcacgt tgctgcacgc ctggagaacc tggcgatgct
    12361 acgcacgctg gtcggtgcca tcggcacgtt cgaggacctg gatttcgatg ccgtggcgga
    12421 cctgcgcctg gcggtggacg aggtgtgcac ccggctgatc cgctcggcca ctccgggcgc
    12481 gacgctggtg gtcgtggtcg acccgcaaga cgacaaattg gtggtggagg cctccgcggc
    12541 gtgcgacacc cacgatgtgg tggcgcccgg cagcttcagc tggcatgtcc tgacgtcgtt
    12601 ggccgacgac gtccaaactt tccacgacgg tcgcgagccc gacgaaaccg gcagtgtctt
    12661 cggtatcacg ctgaccgcgc gacgggcggg cctctagcag gtgactgcga gagctggcgg
    12721 cggttcggtt tcccggccga acgaatatgc cgatgttctg gaaatgttcc gcgagttggc
    12781 gactgtcgcc cccgactcga tggaattcca gcggcaacgg gacaagatcg tggagcggtg
    12841 cctaccactg gctgatcaca tcgcccgccg gttcgagggg cggggggaac cgcgtgacga
    12901 cctggtccag gtggcccggg tcggcttggt caacgccgtg gttcggttcg acgtcgaggc
    12961 cgggtcggac ttcgtttcct ttgcggtgcc caccatcatg ggggaggtcc gccgtcactt
    13021 ccgcgacaac agctggtcgg tcaaggttcc gcggcgcctg aaggagctgc acttgcggct
    13081 tggcgccgcc accgccgatc tttcccagcg actgggcagg gcgcccaccg caaccgaact
    13141 cgcctccgaa ctcggcatgg aacgcgacga ggtcgtcgag ggcctggtgg cgggcagctc
    13201 ttacaacacc ttgtccatcg acacgggcgg cggcagcagc gacgatgacg actcccgtgc
    13261 catagcggac actttgggcg acgtggacac cgggctcgat cgcatcgagg atcgggagtc
    13321 gctgcgcccg ctgctcgagg cgctgcctga gcgggaacga atggtattgg tgctcaggtt
    13381 ttttgagtcg atgacccaaa cccagattgc cgagcgggtc ggcatctcac agatgcacgt
    13441 gtcccgtctg ctggccaaat cgctagcgcg gctgcgcgac cagttgcaat aacgtcagcg
    13501 accgttgggc gcgggcaccg gttggctgca gtagtggctc cgcgcgtggc gtcgtgaccg
    13561 ggagggtgcc gtcggggtca cagacacgca atagccgcga caccgccggg ctcggcgtca
    13621 tggcccagcg cgcctgcaca gccgagcaca ccacgttgat tcggtgtagt gccgaaaagc
    13681 cggcagtacc aatgaaatcc aggccgcgca ggtccaggac cactcggccg gaatgcttga
    13741 tgctgcgttg cacgtaggcc gcgagctggt cggcgttggc ggcgtcgagc tcaccctgcg
    13801 cggtgatcac cgtgccgagg gagcttgagc gggcactgaa ttgagctgta cggcttcgtt
    13861 gccacgattg ggccacagac atgcttctac tccatcgtga ggaggatcgt gctgtgaatg
    13921 acgtcaggcg ggtagtgacg cactatggag ttcgcgtgtc gccgagtccc tgacgttatc
    13981 cgggcggctg tgaactcaac ctgaagaaaa ccctacccgc ggttagccgg ggcgacaacg
    14041 taacccgggt gcgtgccgga agccgctggt cagaccggcc tgcgtcgggc tcgctcaacg
    14101 ggtcacttga tctcggcgag caccgtgccc tgagtaatgg ccgcgccggc ctcgaccgca
    14161 agcccggtga tgacgccgtc cttatgggcg gtgacggggt tctccatctt catcgcctcg
    14221 aggaccacca ccaggtcgcc gacggcgact tcctgtccct cctcgacggc gaccttgacg
    14281 acggtgccct gcatcggcgc ggtcaccgcg tcaccggagg ccaccgcgcc cgagtgcgcc
    14341 ccgcgcttgc gggccttggg cttcttccgg atgacaccgg ccggatcggc ggcaccgttg
    14401 gacaaggcca gatcaccggg cagcgagacc tcgagccggc ggccgcccac ctcgacgacc
    14461 accttctggc ggggccgggc gtcatcgtcg tcaagcggtt cgccgccggt gaacggttcg
    14521 acggtgttgt tccactcggt ctcgatccac cgggtgtgta ccgagaagcc ggagtcgtcg
    14581 ccgatgaacg cggggtcgga caccacggcg cggtggaacg ggatgacggt ggccagccct
    14641 tcgacgtgga actcagcaag agcacggcgc gcccgtccta atgcctccgg ccggttggcg
    14701 ccgtacacga tgagcttgga cagcatcgag tcgaactgac cgccgatgac ggatccggcc
    14761 tcaacaccgg agtccagccg gacaccgggc ccggtgggga tgtcgtaccg ggtgaccggg
    14821 ccgggcgcgg gcaggaagcc acggccggcg tcctcgccgt tgatccggaa ttcgatcgcg
    14881 tgaccgcgcg gcgtcgggtc ttcagtgata tccagcttct cgccgttggc gatcttgaac
    14941 tgctgcagca ccaggtcgat gcccgcggtc tcttcggtga ccgggtgctc gacctgcaga
    15001 cgcgtattga cctcgaggaa cgagatcagg ccgtcctggc cgaccaggta ctcgacggtt
    15061 ccggcgccgt agtagtgggc ctctttgcag atccgcttgg ccgactcgtg gatctccttg
    15121 cgctgcgcgt cggtaagaaa gggtgccggc gcttcttcca ccagtttctg aaatcggcgc
    15181 tgcagtgagc agtcgcgggt gccggcgacg acgacgttgc cgtgctggtc ggcgatcacc
    15241 tgcgcctcga cgtgacgcgg cttgtccagg tagcgctcca cgaagcattc accgcggccg
    15301 aacgccgcga ccgcctcacg ggtggccgac tcgaagagct cggggatctc ctcgatgctg
    15361 cgggccacct tcatgccgcg gccgccgcca ccgaatgcgg ccttgatcgc gatcggcacg
    15421 ccgtactcct tggcgaaggc caccacctcg tcggcgtcct tgaccgggtc gggggtgccg
    15481 gggaccagcg gggcctgcgc acgggcggcg atatgccggg cggtgacctt gtcaccgagg
    15541 tcgcggatcg actgcgggct gggcccgatc cagatcagcc cggcgtccag gacggcctgg
    15601 gcgaagtcgg cgttctcgga caaaaagccg tatccggggt ggatggcgtt ggcgccggac
    15661 ttcgcggccg cgtccaggat cttgccgaag tccaggtagg actccgcgga ggtttggccc
    15721 ccgagggcga aggcctcgtc ggccagccgc acgtggggag catccgcatc gggttcggcg
    15781 tagaccgcca cgctggccag tcccgcgtcg cgggccgcgc ggatcactcg aaccgcgatc
    15841 tcgccgcggt tggccaccag aaccttagag atcctagcga gcctcgagct ggcgtgactg
    15901 gccacagcgc ctcctgcgtt tgatcgtctc taagagattt tcttacaagt cttgtccggg
    15961 gaagtttatg cgccgcgccg taagcggtga tacccggtcc cactcagatt gttgcggggc
    16021 tggtctcgcg caggtgtcgc ttgatccgcg cgagcatcgc cgacattccg cgcaatcgca
    16081 gcgggctgat cagagtggcc agaccaaggt cggtatagaa atcttcgggc accgccagga
    16141 tgtcggcggc cggctgttcg tcaagaccgg cggccaggat cgacgcgaat ccgcgggtgg
    16201 tgggcgcttc ggccggcgcg ctgaagtgca gccgcacccg ctccggatcg ctggcgtcga
    16261 cgtgcaaaaa cagcggggac tgacactcgg gcaccggctc catcgccgcc tctacgagat
    16321 cggacggcag ggctggaagt tcattggcga attccagcag cagtgtcagc ttgtcttggc
    16381 cctggacttc ggcgaagtcg gacaccacct cggccaacgg cgcgggcatg ctcatcgtgc
    16441 gggtacagct cccgggtctt cacccgcgac gatcggcacc cgtacggcgt tgccccattc
    16501 ggtccaggac ccgtcgtaat tgcgcactcc gggcttgccc agcaaatgtg tgagcacgaa
    16561 ccaggtgtgg ctggaccgct cgccgatgcg gcagtagacg acggtcttgt cgtccgggtt
    16621 tataaagtca tagagccgtt ccaattcctc gcggttgcgg aatcgaccgc tgtcgtcgac
    16681 cgccttgccc cacggaatcg accgtgcggt cgggatgtgg ccggcgcgca gcgcgccctc
    16741 ctcggggtaa tcgggcatgt gggtgcgctt gccggtgtac tcctccggcg agcgcacgtc
    16801 gatcagtggt tggctgccga ggatgcccag cacgtcgtct ttgaaggccc ggatgggctc
    16861 gtcgttgcgc tgcacgacgg gatatccggt cgacgtcttg gtcgggacct ccagggtggt
    16921 ctcccggcgt tcggccagcc ataggtcgcg gccgccgttg agcaggcgca cgtcggggtg
    16981 gccgaacaag gtgaagaccc acagcgcgta ggccgcccac cagttgctct tgtcgccgta
    17041 gatcaccacg gtgtcgtcgc gggcgatgcc cttgcggttc atcagctcgg cgaactgtgc
    17101 gccgttgatg tagtcgcgga ctctcgggtc gttgaggtcg gtgtgccagt cgaccttgac
    17161 cgcgccggga atgtggccga cgtcgtagag caagacgtcc tcgtcggatt cgacgatcac
    17221 caggccgggt gtgcccattt tggccgacag ccagtcggcg gtgaccaatc gttcggggtg
    17281 ggaatagacc gacagggagg ggctgggatc gggcggtagc gacacaccca aagcctaccc
    17341 accccaccct ttgcctggtg ctacgcgagc ggattgtccg cccacagcgc ggcgatgggc
    17401 agccccgcgc gggccagcag cgacctcgtg agcgggagcc cgatccccac cacggccgac
    17461 gggtcgccgt cgacgccgtc gatgaaccag ccgcccagac cgtcgagggt gaaacccccg
    17521 gccacccgca acgactcccc ggacgccaag taagcctcca ggtcgtcagg agatggcgtc
    17581 ccgaaatgca ccgtggtggt cgatgccgcc gaatcacgat ggaccgtctc gttgtccagc
    17641 agtcggatga cgcaatggcc ggtatagagc tggccggcgc ggcccgacat tgaccgccac
    17701 agttgccgcg cggcgccggc ggtgtgcggc ttgccgcata gccggccgtc gatgtagagc
    17761 atggaatcgc acccgatgac cacgcaatcc gccgcgacgg cgcgatccag cttgccggcc
    17821 acttgctcgg ccttggccgc cgcgagggcg cacaccacct cggagggcga cgcgtctggc
    17881 cccagacccg cggtgaccac atcctcgtcg accccggaca ccatgaccag cggatcgatg
    17941 ccggcctggc gcagcacttt gaggcgcccc ggggaggcgg accccagcac catgcgggtc
    18001 atcgctgcat gtgcatgcgt tcctgcagag tgattcgctg gtagttggag atcgcgtagc
    18061 gcagcctgtc gaccggcagg ccccagcggg tgcgttcggg ttgtcccggt tccgggccac
    18121 ggcccgcgct gcccagcacg acgatcagcg cggccagttc ctcgtcggtg ggctgaccct
    18181 tgagtatctg gatgtgcggc tcgtgcggat gctggggggc cgtcgtatcg gtcatagctg
    18241 aattgttcgt cacaggggta tgttcccgtg cttcttgggc ggcaggtgcg agatcttgcg
    18301 ttccagcagg cgtagcgccg tgccgatgta cccgcgggtg tgtgacggcg ggatcaccgc
    18361 gtcgacatat ccgcgctcgg cagcgacgta ggggttgacc aacgtgtcct cgtactcctc
    18421 ctgcaactgc agacgcagcg tgtcgacgtc ctggccctgg ttggcggcct cggccagctg
    18481 ctggcggtac acgaagccga cggctccgga ggcgcccatc accgcgatct gggcggtcgg
    18541 ccaggccagg ttgacgtcgc agcccatgtc cttggagccc atgacgcagt aggcgccacc
    18601 gtaggcctta cgggtgatca ctgtgatctt cggcacggtc gcctcgccgt aggcgaacaa
    18661 cagcttggcg ccccgccgga tgatcccgtt gtattcctga ccggtaccgg gcaggaaccc
    18721 cggaacgtct accagcatga cgatcgggat gttgaagcag tcgcaggtcc gcacgaagcg
    18781 ggcagccttc tccgaggcgt tgatgtccag acagccggcg aaatgggtcg gctggttggc
    18841 cacgatcccg accggccggc cctcgatccg gccgaacccg acgacgatgt tctgcgcgta
    18901 accggcctgg acctcgagga actcgtcctc gtcgaggatg cgggtgatca cctcgtgcat
    18961 gtcgtaaggc tggttgggca agtccggcat caacgtgtcg agctcgagat cctcctcggt
    19021 gaggttgtcc tcgatggcgc cctcgggaat gggctcggca taccggggcg ggtcggtgaa
    19081 gttgttcggc ggcaggtagc tgagcaggtc gcgaacccag tcgaaggcgt cctgttcgcc
    19141 cgatgccacg taatgcgcgg tacccgactt ggccatgtgc gtgtgggcgc cgccgagctc
    19201 ctccatggtg acgtcctcgc cggtcacggt cttgatgacg tccggcccgg tgatgaacat
    19261 ctggctggtt tggtcgacca tcaccacgaa gtcggtgagg gcgggggagt agacgtggcc
    19321 gccggcggcg gcgcccatga tcagcgagat ctgcgggatg actccggacg ccacgatgtt
    19381 gttgcggaag atccggctgt acaggcccag cgagacgacg ccttcctgga tgcgcgcgcc
    19441 cgcgccgtcg ttgatgccga tgagcgggcg gccggtcttg atcgccagtt cctggacctt
    19501 gacgatcttg tcgccgtaca cctcgccgag gctgccgccg aacaccgtga cgtcctggct
    19561 gaagatgcag acgtctcggc cgtcgatggt gccgtagccg gtgaccacgc cgtctcccag
    19621 cgggcggttc tcggcgaggc cgaagttggt gctgcggtgt cgggccagcg cgtccagttc
    19681 gacgaacgag tcgtcgtcca acagggcgta gatccgttca cgggcggtca gcttgccctt
    19741 ggcgtgcacc ttctcgatgg ccgcctcacc gaccggatgc agcgcctccg cgcgccgctt
    19801 gtgcagttcg gccagcttgc ctgcggtggt gtggatgtcg atggtgtgct cggcggcggg
    19861 ctcggcggtg tggccggtaa cgcttgtcat gggactcgat gttatcggca gcgcgtgcgg
    19921 ggacgtttag gctgggcttg acacaggaga aatgggtgac ggaccgggag ctattcaggc
    19981 agcccttgga cgcggccgcg ctgcgggccg agctgatcgg gaccgggctg ggttggcgcc
    20041 agctcgacgt cgttgagcga accggctcga ccaacgccga cctgttggcg cgagcggcat
    20101 cggggaccga cgtcgccggg acggtgctga tcgccgagca ccagaccgcc ggtcgcgggc
    20161 gtcacggccg cgcatggtcg gccagccctc gagcccagat cacgatgtcg gtgggcgtca
    20221 gtgtcgtcga cgtcccggcc gaggggtggg gctggttgtc gttggccacc ggtgtggcaa
    20281 tcgtcgacgc gctggccccg gtgctggcag cgatcgggat tcaggcgggc ctcaagtggc
    20341 ccaacgacgt cctggccggc ccagcaggcg cgccaggcaa gctggcgggc atccttgccg
    20401 aggtcgcgcg gccggtcgtg gtaatcggcg tggggctcaa cgtgacgcag gcccccgagg
    20461 aggtcgacgg tcccggcgcg acctcgctgc tcgacctcgg cgttgccgca cccgaccgca
    20521 accagctggc ctgcgcgcta ttgcgggaac tcggcaggcg gatcgtcgcc tggcgggccg
    20581 cccgcggggc cgactgggcg ctggccgccg actaccgcgc gcgcagcctg acgatcggca
    20641 cccgcgtgcg cgcctatctg cccggcgaca aggagctcgt cgggaccgcg agcggcgtcg
    20701 acgaccacgg ccggttgtgc ctggaaacgg cgagcggcca aaccgccgtg gtttcggccg
    20761 gtgacgtggt gcacttgcgc tagttcgcgc agccgaagag aaacgatgtg aggtcgcgag
    20821 agtgagctat ccggacaatg ccctggcgcc cggcgaacag gtcgtcgtgc accgccaccc
    20881 acactggaag cgtttgatct ggcccgttct ggtgctcatc ctggtgaccg ggctggcggc
    20941 gttcgggtcc ggctacgtca actccacgca ctggcaacag ctcgccaaga acatcgtcta
    21001 cggcgtcatt tgggggattt ggttgatcat cgtcggctgg ctgacgctgt ggccgttcgt
    21061 gagctggctg accacccatt tcgtcgtcac cagccggcgg gtgatgtttc gtcacggagt
    21121 gctgacccga agcgggatcg acatcccgtt ggcgcggatc aacagcgtgg aattccggga
    21181 caggcttttc gagcggatgt ttcgcaccgg gacgctgatt atcgagtcgg cgtcacaaga
    21241 tccgctggag ttctacgaca ttccgcgact gcgcgaggtg cactcgctgc tgtatcacga
    21301 ggttttcgac accctgggct ccgaggagtc gcccagctga gccaaccggc tcgccctgtt
    21361 gcgccacgag cgcagcaggg tgacatttcc ttccaagccc tgcaagcctt cttcgaagcc
    21421 gccctcgaag gcgtcctcga agacctcggc gataccgccc gcggtgagtg cggattccag
    21481 cgccttgtcg gggctgcggg cgaactcctc gggaaacacc cagcgtcgga acgcccagaa
    21541 ccggaacgcc atctgcagca ggttgccgat gatgtaggcc gagatgaagt cagcgatgtt
    21601 ttccaaagtc agtgacacca tcggcacccg caactgcagg acgtagctcg agacccacag
    21661 cggcgccatg ctcagcagca cccctactcc gctgaacgcg aaaaacagca gcgcctcgtg
    21721 atggcgctcg cgcccgccgc ggtcgcggaa actccactcc cggttcaaga cgtaggacgc
    21781 gatgaccgcg acgatgcccg cgatcacctt ggcggtcacc ggcttggttt cgagaatcgt
    21841 cagcttcagc gtgtagaaaa tcactgagtc gataacgaac gtggtgccgc cgacgatggc
    21901 gaatttgatg agttcatgat ggcgctgcgc gtagggctga acagcctggg gcaggcgcgc
    21961 tatcgtggca tcggcgaagg acacaacacg tcagtgtacg gacgtccgca aaaacgacgc
    22021 aaaaccgcga cataacgatt ggggtaacgg ccggtccgcg cacccctgaa cacgacactg
    22081 tgcgccatga caccatgatg gccgtgccga gtagacgccc gccggaggtg gcccctctag
    22141 ttgcgatggt cggcggcggc cagctcgccc ggatgaccca ccaggccgca atcgcgctgg
    22201 ggcagacgct gcgggtgctg gccacgtcca ccgacgaacc ggccgcgcag gtcacgcccg
    22261 acgtggtgat cggctcgcat accgatctgg aagacctgcg ccgggtcgcc gtgggcgccg
    22321 acgtgctgac cttcgaccac gagcacgtcc cgaatgagct gctggtgaag ctggtcgccg
    22381 aaggcgtcaa cgtggcgccg ccgccgcagg ccctggtgca cgcccaggac aagctcgtca
    22441 tgcggcggcg gttgcaggcc ctcggcgtcc cggtgccccg ctacgcggaa atccggagca
    22501 tggacgagct cgatgctttc gcgcggcgca tagccggccc cgtggtcgtg aaagcggtcc
    22561 gcggcggtta cgacgggcgt ggggtgcgga tggcccgcga cccttcgcat gcccgcgaga
    22621 tcgcggccgc cttcctggcc gatggggtgc cggtgatggc cgaggagcag gtgaacctgc
    22681 gtcgcgagtt gtccgcgctg gtagcccgct cgccgttcgg ccagggcgcg gcatggccgg
    22741 tcgtggagac ggtgcaacgg gacgggatct gcgtgcaggt gatcgccccc gccccggacc
    22801 tgcccgacga cctggcctcc gccgcccagc agctggcatt gcggctggcc ggcgagctcg
    22861 gtgtggtggg tgtgctcgcg gtcgagctgt tcgagaccac cgaccgtgcg ctgctggtca
    22921 acgagctcgc gatgcggcca cacaactccg ggcactggac catggacggg tcgcgcacca
    22981 gccaattcga gcagcacctg cgcgcggtgc tggactatcc gctcggcgac accgaagtca
    23041 tcgcgccggt gacggtgatg gccaacgtgc tcggcgccgc gcggcagccg gcgatggccc
    23101 tcgacgagcg attgcaccac ttgttcgcgc ggatgcccga cgcccgcgtc cacctctatg
    23161 ggaaggcgga acgagcgggt cgcaagattg gtcacatcaa cttcgtcggc tcgaacgcca
    23221 cggacctggg gatgctgcgt gaccgcgcgg agctagcggc acactggttg tcgcacggac
    23281 agtggacgga cggatgggat tcacaccgcg ccggcgacga tgcagagcga agcgatgagg
    23341 aggagcggcg cccatgacac gcgccggcga cgatgagaag gtggggcgcc catgacccaa
    23401 cagtccagcg gggctgaacg tcctcgcgtc ggcgtgatca tgggcagcga cagcgactgg
    23461 ccggtgatga tcgatgccgc cgaggccctg gccgaattcg acatcccgat cgaggttcgg
    23521 gtggtctcgg cgcatcgcac ccccgacgtg atgctcgatt acgcccgcgg cgcggccgac
    23581 cgcggccttg aggtgatcat cgccggcgcc ggcggcgccg cccacctgcc gggaatggtc
    23641 gcctccgcga caccgctgcc ggtgatcggc gtgccggtgc cgctggcccg gttggacggc
    23701 ctggactccc tgctgtcgat cgtgcagatg cccgccggcg tcccggtggc tacggtatcc
    23761 atcggcggcg cccgcaacgc cggtttgctg gccgtgcgca ttctggcatc gtccgacccg
    23821 cagttgcggg cgcggatcgc cgcgttccaa cggcggctgg ccgacagcgt gcgcgccaag
    23881 gatgcggcgc tgcagcagct gcacgataaa gtcagcgacg agtagctaag gagacaaaga
    23941 tggctggatg ggccggaaac ccgtcgtttg acctgttcca actgcccgag gagcaccagg
    24001 aatttcgggc ggcgatccgc gcgttggcgg aaaaagagat cgcgccgtat gccaacgacg
    24061 ttgacgagca agcccggttc ccggaagagg cgctggcggc gctgaatgcg tccggcttca
    24121 acgccgtgca tgttcccgaa gagtacggcg gccagggtgc ggactcggtg gccgcttgca
    24181 tcgtgatcga agaagtcgcg cgcgtcgacg cttccgcctc gctgatcccg gcggtgaaca
    24241 agctcggcac catgggtctg atcctgcggg gctcggagga actgaagaag caggtgttgc
    24301 cgacgctggc caccgagggc gcgatggcgt cctacgcgtt gagcgagcgc gaagccggca
    24361 gtgacgcggc gtcgatgcga acccgggcca aggccgacgg cgacgactgg atcctcaacg
    24421 gcgccaagtc ctggatcacc aacggcggaa agtcgacctg gtacacggtg atggcggtga
    24481 ccgacccgga caagggcgcc aacggcatct cggcattcat cgtgcacaag gacgacgagg
    24541 gtttcagcgt cggtccgaag gaaaaaaagc tcgggatcaa gggttcgccg accacggaac
    24601 tgtacttcga gaactgccgc atccccggcg atcgcataat cggcgagccg ggtaccggct
    24661 tcaagaccgc gctggccacg ctggaccaca cccgtcccac catcggcgcc caagccgtgg
    24721 gcatcgccca gggcgcggtg gacgccgcca ttgcttacac caaggaccgc aagcaattcg
    24781 gcgagtcgat cagcaccttc caggccgtgc agttcatgct ggccgacatg gcgatgaagg
    24841 tggaggcggc acggctgatg gtctactccg ccgccgcccg cgccgagcgc ggtgagccgg
    24901 acctgggctt catctcggcg gcgtcgaaat gcttcgcctc cgacgtggcg atggaggtca
    24961 ccgtcgacgc cgtgcaactg ttcggtggcg ccggctacac catcgacttc ccggtcgagc
    25021 ggtttatgcg cgacgccaag atcacccaga tctacgaggg caccaaccag attcagcgcg
    25081 tggtgatgtc gcgggcgctg ctgcgctgac ccgggttggg tcggcatcac cgtccgtggc
    25141 ggcagctcag cgtcagcgaa taggatcgac gactacgacc agggccgcgt tatgggcaac
    25201 gattcgtgat ggcgtaaccc acgcgtaagg tcttcggcgg cgcgcccgcc gacgggttgg
    25261 gacgtcgtcc acacgagcga aggcatcgcc atggccaaag ggggtgtgtg aggtgagaga
    25321 actggtttgc cccgcgcgcc cacgcatgac cgggatttga tgcccgtgcg tgcaccagtc
    25381 atcccggtag cgcattggca gtcactgtcg ttgctcttgg tcgaagacga ccgcgccgat
    25441 gcggtgctcg tggaggacgt gatcgccgac gcggtggccg atatccgggt tatctgggcg
    25501 cagtcgatgg cacacgccga gcgcgagctg gcgtcggcgc ggcccgattg cgttctgctg
    25561 gacctgaatc tgccagacgc caacgggatc gacgcgctgg atcgcatcgc gaaacgcgat
    25621 gccactgttc cgatcgtcgt gctgaccgga ctgaacgacg aatacttcgg agcctccgcc
    25681 gttgccgccg gcgcgcagga ctacctggtc aaggggcgcg tcgaacctga aatgttgcgc
    25741 cgcgcgctgc tttacgcgat cgagcgcaaa cgcgccgagc tcatttccgc ggacctgcgc
    25801 gcgagccggc agcgggcgcg cgagaacgcg ctgctggaac gcggcctatt accctccccg
    25861 ctgctgctcg acgaccccgg cgtcgacatc gtcgcccgat accggcccag ccgcgaggac
    25921 gcgctgctgt gcggagactt ctacgacgtc gtgcaaacgc cggaccgggt cgtgcatgtc
    25981 ctgatcggcg atgtcgcggg ccacggcccg cacgaggcgg ccctgggcgc cgcgctgcga
    26041 atcgcgtttc gcgcgctcac cttggccggc gtccagggag tcgaactgat gcggctgctc
    26101 gagcgggtgc tgcactccga gcgaaccgcc accggagtct tcgcgacggt gctcagcctt
    26161 gaaatctctc ccgattgccc gcgcatcacc accgtccgcg ccggtcatcc gggcatgttg
    26221 cggcaaaacg atggcggcgt ggaatggatc gagccgcccg gggggccggc gctgggactg
    26281 cgcgccgacg actggccgtc gcacgagctg gacttgcccg ccggccaggg tctgctgtta
    26341 ctgaccgatg ggctcttcga gggctattcg gggcggggca acaagcgtct gggcgaggat
    26401 ggcttgctcg cgctggcccg gtcgcacgcg cgtctgcccg gcccggcgtt cgtcgacgcg
    26461 ctgatcgatg gcgctcagga actcgcccag ccacgcggtg gcctgaccga cgacatagcc
    26521 gtgctgcgcg tcgagcggac cacaacgtga gctccgcgtc gcgcccctcg cgcctgtcgc
    26581 agctcaccgt gcgcggctgg caggccctgc tgctgtcctg catgggcgtg atggtgctcg
    26641 ccggtgcgct ggtcggtgcc gtcttgctgt atcgcaccga cgagacgtcg cgcgagctga
    26701 ccgacgacat tcagccggcc cggttggcgg cctaccagct gcagtcggcg ctgcgcgacc
    26761 aggaaaccgg cgtccgaggc tacgtgatcg ccgccgatcg gcagttcctc acgccctact
    26821 acgacgggca gcacgccgaa cgcgaagcgg ccgaagacgt ccggcgccac gtggggcagc
    26881 gcgcggacct ggtcggcgac ctggacgcga tcgagaaggc cgccgcgaac tggcggacaa
    26941 actatgccga gccgctgatc gcgagcgtgg cccccaacgg tcgcaatgtc gcgaacctgc
    27001 cggcggcgga acgcggcaaa gtcgaattcg accatttacg tgcgcttttc gatacccaga
    27061 acgcgaatct gaccgcggcc tgggcgcacg cggccgacga cctcaaagac accgacgcct
    27121 ggcgcgaccg ggtgctgggc gccatggtgg tgctgttctt cggcaccgcg gccctgctgg
    27181 ggctcctgca acgggacgtg gtcacccgcc cgctcgccgc gctggctgcg tcatgccggc
    27241 ggatcaccga gggcaacttc ggcga
//
LOCUS       DASSGQ010000252         3570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1141909,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000252 DASSGQ010000000
VERSION     DASSGQ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141909"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VFI55_11140"
     CDS             complement(<1..630)
                     /locus_tag="VFI55_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET7074621.1"
                     /translation="MFLGIIVNPLARRNRGAADRSAELRRIVGRWGEVHETSAVEDLH
                     GVVEQLAPRVSHLVCYGGDGSLNWLINEAEQCVSDPERWPTFVPSNGGSVNAVARKAR
                     VRGRPEAILRALTAAAETDRPPPEVFLDTLTLDGETADGAKFHRLCFGLAAGGVGNRF
                     YEKYYDNQTHGRSDVVRVIARSFGDYVTNKVVPGRSNKPNYASSLFAPTH"
     gene            911..1315
                     /locus_tag="VFI55_11145"
                     /pseudo
     CDS             911..1315
                     /locus_tag="VFI55_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872742.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            1537..2463
                     /locus_tag="VFI55_11150"
     CDS             1537..2463
                     /locus_tag="VFI55_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7074622.1"
                     /translation="MSAAHIAAFYALCAALASAIGNVIRQRSAQEVTDKPVGHLTLFG
                     MLLHDTRWWLGGLGDIASYCLLALALDDGSVLLVMSLQVTALLFALPIYARLTRHPVT
                     RQEWMWALLLALALATAIAVGQPVGGQDRASSQTWLVVALVMGPPLVLCLLGARIWSD
                     RPAAAVLLAAVAGSLLAVFAVLMKGIVDIVEHNAGQLWHTPELYAWVFCGVAGMIYHQ
                     SAYRAGALTASLPTIIVAKPVVGGVLGVTVLEEAVRSDGPELLVLAVAAVVVIVATVG
                     LARGEAATMAAGEGRDVKITDQPKVPPQRLSG"
     gene            2807..3535
                     /locus_tag="VFI55_11155"
     CDS             2807..3535
                     /locus_tag="VFI55_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005087588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET7074623.1"
                     /translation="MRRLILLLVAAALVVACSACDGARVAVRPVDTTVPAAPGVPPVS
                     DAARAAGFVDVRSVVPDAIIDLHYASTNNFTGIQLYPSDARCLVHQSMAPGLAAAAAA
                     LRPQAHSLVFWDCYRPHDVQAKLFSVVSNPAWVARPGPYAHSHESGRSVDVTFTTLQA
                     QCPPERQVDGFCLADMGTDFDDFSSQAKAFATQGVSADAQANRARLRDAMTYGGLTVY
                     SGEWWHFDGPGAAVDRPILDVPVD"
BASE COUNT          533 a         1154 c         1198 g          685 t
ORIGIN      
        1 gtgcgtcgga gcgaacaggc tggatgcgta gttgggcttg ttcgagcggc cggggacaac
       61 cttgttggtg acgtagtcgc cgaacgagcg agcgatcact cgaacgacgt ccgaacggcc
      121 atgagtttgg ttgtcgtagt acttctcgta gaaccggttg ccgacacctc cggcggccag
      181 cccaaagcag agccgatgga atttcgcgcc gtccgcggtt tcaccgtcga gcgtcagagt
      241 gtccaggaac acttcgggtg ggggccgatc cgtctcggca gcagccgtca gtgcccgcag
      301 aatcgcttcg ggccgtccgc gtactcgcgc cttgcgcgcg acagcgttga cactgccgcc
      361 gttgctaggt acgaatgtcg gccagcgttc cggatcgctg acgcattgct cggcttcgtt
      421 gatcaaccag ttcagcgacc cgtcgccgcc gtagcacacg aggtggctga cccgcggagc
      481 gagctgttcc acgaccccgt ggaggtcctc cacggcggag gtttcgtgca cctcgcccca
      541 ccggccgaca atgcgccgca attcggcgct gcgatcagcc gcaccacggt tcctgcgtgc
      601 gagcgggttg acgataatcc ctagaaacat ccggaggtca cagacgcatg cctaccggaa
      661 cgcatcgccg tcgggccgac gggcgacggt ttcagcggga gctaacgagg cgggcgcatc
      721 gagtcccaac tgaataactc ctccgcaggg gactggggcg cgctcaagtg ggattcggcg
      781 cccttcccgc acgatacgcg cgacgtctcg gatgagcccc gcaccggtca gccaggccaa
      841 caccatcgcg tcggctcaag cggcgaatcc tcgtcatacc ccctcgcaac ctcggattag
      901 tgtgtgcccc tacggccagt cgaagctcgc caacctgctc ttcaccagcg agctgcagcg
      961 gcgcctggac actgtcgatc ccccgcaagc gggtggcgcc cccacttcgc tgcgcgctgg
     1021 ccgcccaccc cggctggtcg cacacgaacc tgcaaggccg ctccggccac agattgagcg
     1081 acgcggttgt gctggctgcc gatcgtgtgg tgtccaccga tgccgacttc ggcgcccgcc
     1141 aaacgttgta cgcggtatcg caggatctac cgggcaacac gttcgtcggt ccgcgattcg
     1201 gctatggggc cgcagccagc cgctccggcg cagctggctg gccaagcgcg caaccaccgc
     1261 cgccgccctg tgggaactgc ccgaacagct caccggcacc aaatttccgc tctgagacgg
     1321 gtgcggtgag ccccgcgcgg ttgcccgacg tgcagccttg gacggccgtc ccctcgactc
     1381 gaggctaaat tgggagggta gccagtaagg cggtttcagc gcggggtgcg caaaggcggg
     1441 gcgactgtca gcgcaagcga accgaccgcc agaagcggcg tgtgatgtgg gtatacgtgc
     1501 ggcttacggg ccggacgtcg gcgaggaggt caccccatgt cggcagcgca catcgcggcg
     1561 ttttacgccc tgtgcgccgc gctggcatcc gcgatcggca atgtgatccg tcagcggtcc
     1621 gcgcaggagg tcaccgacaa accggtcggc cacctgacgc tgttcggcat gttgttgcac
     1681 gacacccgct ggtggctggg cggtttgggg gacatcgcca gctactgctt gcttgctttg
     1741 gccctcgacg acggctcggt gttgttggtg atgtcgctgc aggtgacggc gttgcttttc
     1801 gccttaccga tttacgcgcg gttgacccgt catccggtaa cccgccagga gtggatgtgg
     1861 gcgctgttgc tggccctggc gttggcgaca gccatcgcgg tcggacagcc ggtgggtggc
     1921 caggatcggg catcgtcgca aacctggctt gtagtggccc tcgtcatggg tccgccgttg
     1981 gtgttgtgtt tgctgggtgc ccggatctgg tcggatcgtc cggctgcggc ggtgctgctg
     2041 gcggcggttg ccggctcgtt gttggcggtg ttcgcggtgc tgatgaaggg cattgtcgac
     2101 atcgtcgaac acaacgctgg tcagttgtgg catacacccg agctgtatgc ctgggtgttt
     2161 tgcggggtgg ccgggatgat ctatcaccag tcggcgtatc gcgccggtgc tctgaccgcc
     2221 tcgttgccga cgattatcgt cgcaaagccc gtggtgggcg gtgtgctcgg ggtcacggtg
     2281 ctcgaagagg ctgtgcgatc cgacggcccg gagttgcttg ttctggcggt ggcggcggtg
     2341 gtggtgatcg tcgcgacggt gggactcgcc cgcggtgagg ccgccaccat ggcggcgggc
     2401 gaggggcgtg acgtgaaaat caccgaccag ccgaaggtgc cgccgcaacg cctgagcggc
     2461 tgacgctcgg aacctttgct gcgtaggaga gcgctgtctg cttgtcgatg gtctgcggaa
     2521 tgtggtcctg cactccggcg aagtcggccc ccaccgtgtg tttcggcgcc gtggcgcgct
     2581 cggtccttcg cggcgtagtt gagcgtgaac accttgtcta tatcggagtc ggcttcatcg
     2641 atttcgtcgc ggcctcacga cggaccggat gagcgcgggg agtgatagct atcgggtcgc
     2701 gtccggcacc cgaggccgcg gcacgcgcga gctcgagttg ttcactgtat tcggttgtgc
     2761 gggtcgttcc ggggagacga atcgcgacaa tgtttaaata cctcgggtgc ggcggttaat
     2821 tctgctcttg gtcgctgccg cgctcgtcgt cgcctgtagc gcctgcgacg gtgcccgcgt
     2881 tgccgtccga cccgtcgaca ccaccgtccc ggctgctccc ggcgttcctc ccgtcagcga
     2941 cgcggctcgc gcggccggat tcgtcgacgt ccggagtgtg gttcccgacg ccatcatcga
     3001 tctgcactac gcgtcgacga acaatttcac cggcatccag ttgtacccct ccgacgcccg
     3061 ctgcctggtg caccaatcga tggccccggg gctggcggcg gccgccgcgg cgcttcgccc
     3121 gcaggcccac tcgctggtgt tctgggactg ttatcggcca cacgacgttc aggccaagct
     3181 gttcagcgtc gtctccaacc cggcttgggt ggcccgcccg ggtccgtatg cgcacagcca
     3241 cgagtccggg cgttcggtgg acgtgacttt caccaccctg caagcgcagt gcccgcccga
     3301 acgacaagtc gacggctttt gcctggccga catgggcacc gacttcgacg acttctcctc
     3361 gcaggcgaag gcattcgcaa cccagggtgt cagcgccgat gctcaggcga accgcgcgcg
     3421 gctgcgtgac gccatgacct acggcgggct gaccgtgtac tccggcgagt ggtggcattt
     3481 cgacggtccc ggtgccgccg tcgaccgtcc cattctcgac gttccggtcg actagtgttc
     3541 ataactcaca tactgagacg ctaatttcat
//
LOCUS       DASSGQ010000253         2632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_898569,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000253 DASSGQ010000000
VERSION     DASSGQ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898569"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2010
                     /locus_tag="VFI55_11160"
     CDS             <1..2010
                     /locus_tag="VFI55_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET7074624.1"
                     /translation="VDEEHGGSGYGLPELVVVIEELGRAVAPGPFVPTVIASAVIAKD
                     GTAEQKSRLLPGLIDGTVTAGIGLDGQVQVADGPQNRIADGDAGIVLGAGLAELLLIA
                     AGDDVLVLERGRAGVFVDVPANFDPTRRSGRVRLENVSIAAEDILPGARESALARART
                     LLAAEAVGGASDCVGAAVDYAKVRRQFGRTIATFQAVKHHCANMLVAAESAIAAVWDA
                     SRAASEDEQQFRLIAAVAAALAFPAYARNAELNIQVHGGIGFTWEHDAHLHLRRALAI
                     AALFGGDAPARDVFDRTAAGVVRANSLDLPAEAEQLRTRIRADAAEITALDTEAQRDR
                     LIETGYVMPHWPEPWGRAADAVEQLVIEEEFRAAGIKRPDYSITGWVILTLIQHGTPW
                     QIERFVEKALRQDEVWCQLFSEPEAGSDAASVKTRATRVDGGWKLNGQKVWTSGAQYC
                     SRGLATVRTDPDAPKHAGITTVIIDMKGPGVEVRPLRQITGGSEFNEVFFNDVFVPDE
                     DVVGAPNSGWTVARATLGNERVSIGGSGSFYEGLAAKLVQLAQRNADRLAGAEVRVGY
                     YLAENHALRLLNLRRAARSVEGAGPGPEGNVTKLKLAEHMVEGGAISAALVGPEVALT
                     DGPGAVIGRMMMGARGMAIAGGTSEVTRNQIAERILGMPRDPLIN"
     gene            complement(2046..2390)
                     /locus_tag="VFI55_11165"
     CDS             complement(2046..2390)
                     /locus_tag="VFI55_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HET7074625.1"
                     /translation="MPGAPTSPLLIEHCDACARWVHPATGACRDCGGPLVPRPVSGRG
                     TVFTYTVNHHPYNPDLPTPYVIAIVELDEQSGLRVAANIVGCEPDSVKCGMPVGIQPE
                     LGSGGAPLFAPA"
     gene            2441..>2632
                     /locus_tag="VFI55_11170"
     CDS             2441..>2632
                     /locus_tag="VFI55_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET7074626.1"
                     /translation="MTYFEKDAILSGIGISRIGRRTGIPGVELTMEAVRAAIADAGLM
                     PADVDGIATLGDTPAEEVTA"
BASE COUNT          427 a          858 c          962 g          385 t
ORIGIN      
        1 gtcgacgaag agcacggcgg ttcgggctat gggttgcccg aactcgtggt ggtgatcgaa
       61 gagctcggcc gcgcggtggc gccgggaccg ttcgtgccga ccgtcatcgc atcggcggta
      121 atcgccaaag acggcaccgc cgaacagaag tcgcggttgc tgcccgggct gatcgacgga
      181 accgtaactg ccggcatcgg tttggacggc caggtccagg tcgccgacgg tccccaaaat
      241 cggatcgccg acggcgacgc cgggatcgtg ctgggcgccg gcctcgccga gctgctgctg
      301 atcgccgccg gtgacgacgt gctcgtgctc gagcgtggcc gcgccggcgt gttcgtcgac
      361 gtgccggcca atttcgatcc gacccggcgt tccgggcgcg tgcgtctgga gaacgtgagc
      421 atcgccgcgg aggacatctt gccgggggcg cgggaatcgg cactggcccg ggcgcggacg
      481 ttgctcgccg ctgaggcggt gggcggcgcg tccgattgcg tgggcgccgc cgtcgactat
      541 gcgaaggtcc gccgccaatt cggccgcacc atcgccactt tccaagctgt caagcatcat
      601 tgcgcgaaca tgctggtggc cgccgagtcg gcgatcgccg cggtctggga cgcgtcccgg
      661 gcggcatccg aggacgagca gcagtttcgc ctgatcgccg ccgtggccgc ggcactggcg
      721 tttccggcct acgcccgcaa cgccgaactc aacattcaag tgcacggcgg catcggcttc
      781 acctgggagc acgacgcgca tctgcacctg cgccgggcgc tggcgatcgc ggcgctgttc
      841 ggcggcgacg caccggcccg ggacgtcttc gaccgcacgg cggccggcgt tgtccgggcg
      901 aacagcctgg acctgccggc cgaggccgag caactgcgca cccgcatccg tgccgacgcc
      961 gccgagatca ccgcgctgga caccgaggcg cagcgcgaca ggctgatcga gaccggatac
     1021 gtgatgccgc actggcccga accctggggt cgcgcggcgg acgcggtgga gcagctggtg
     1081 atcgaggagg agttccgggc ggcaggcatc aagcgccccg actactccat caccggatgg
     1141 gtgatcctca ccctgatcca gcacggaacc ccttggcaga tcgaacgatt cgtcgagaag
     1201 gcgctgcgtc aagacgaggt ctggtgccag cttttctccg aacccgaggc gggttcggat
     1261 gcggcgtccg tcaagacccg cgccacccgg gtggacggcg gctggaagct caacgggcag
     1321 aaggtgtgga ccagcggggc gcagtactgc agtcgcggcc tggccaccgt acgaaccgac
     1381 cccgacgctc ccaagcacgc cggcatcacc acggtgatca tcgacatgaa gggtccgggc
     1441 gtcgaggtgc ggccattgcg gcagatcacg ggcggctcgg aattcaacga ggtgttcttc
     1501 aacgacgtgt tcgttcccga cgaggacgtt gtcggggcgc ccaactcggg ctggacggtc
     1561 gcgcgagcga cgctgggcaa cgagcgggtc agcatcggcg gcagcgggtc gttctacgag
     1621 ggtttggcgg cgaagctggt gcagctggct cagcggaacg cagatcggtt ggcgggcgcc
     1681 gaagttcggg tcggctacta ccttgccgaa aatcacgcgc tgcggttgct gaatctgcgg
     1741 cgtgccgccc gcagcgtcga aggagcgggg cccggtccgg aaggcaacgt caccaagctg
     1801 aagctggccg agcacatggt cgagggcggc gcgatctcgg cggcgctggt ggggcccgag
     1861 gtcgcgctga ccgacgggcc cggcgcggtg atcggccgaa tgatgatggg cgcccgcggc
     1921 atggcgatcg ccggcggcac ctccgaggtg acccggaatc agatcgccga gcggattctc
     1981 ggcatgccac gcgacccgct gatcaactag ccgcgacgtc cggacgagca gggggagcgc
     2041 ggccgtcagg ccggcgcgaa caacggcgcg ccgccgctgc ccagttcggg ttgaatgccg
     2101 acgggcatcc cgcacttcac cgaatccggc tcgcagccaa cgatattggc ggccacccgc
     2161 agcccgctct gttcgtcgag ttcgacgatg gcgatcacat aaggggtggg gaggtcgggg
     2221 ttgtaggggt gatggttgac cgtgtaggtg aacaccgtgc cgcgcccgga aaccggccgt
     2281 ggcaccaggg gaccgccgca gtcgcggcac gcgcccgtcg ccggatgcac ccagcgcgcg
     2341 cacgcatcgc aatgctcgat gagcagcggc gatgtcgggg cgcctggcac gaccaataca
     2401 gtacactcta ttaattctgg gtggctaacg ggcggtgcac atgacgtact tcgaaaaaga
     2461 cgcgatcctg tcgggcatcg gcatttcgcg gatcggtcgt cgcaccggca tcccgggggt
     2521 cgagctgacc atggaggcgg tgcgggccgc catcgcggat gccgggctga tgcccgccga
     2581 cgtcgacggg atcgcgaccc tgggcgacac cccggccgaa gaggtgaccg cg
//
LOCUS       DASSGQ010000254         4146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1415548,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000254 DASSGQ010000000
VERSION     DASSGQ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415548"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VFI55_11175"
     CDS             complement(<1..951)
                     /locus_tag="VFI55_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HET7074627.1"
                     /translation="MDMPNVTAMAAGGATQLSFADVGQSWDPAATPIDEVSLRETTFV
                     VVDLETTGGRTKGSDGAPPDAITEIGAVKVRGGVVLGEFATLVDPQRAIPPQIVQLTG
                     ITTAMVCDAPTIDAVLPMFLEFAGSAVLVAHNAGFDIGFLRAAAQHTDIAWPRPRVLC
                     TVRLARRVLSREEAPSVRLAALARLFAVATQPTHRALDDARATVDVLHALIERVGNQG
                     VHTYADLRSYLPDVTPTQRRKRVLADGLPHRPGVYLFRGPAGEVLYVGTAVDLRRRVG
                     QYFTGADPRGRMKEMVTLTSAVDHVECAHSLEAGVRELRML"
     gene            1071..1325
                     /locus_tag="VFI55_11180"
     CDS             1071..1325
                     /locus_tag="VFI55_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074628.1"
                     /translation="MAPNNDPAHPDRGAPRADAPVVIDCDECAVRGPGCRDCVVSVLL
                     GIPETLLGDECAALEALADAGMAPRLRLVPIRRDRGFGVA"
     gene            1525..2685
                     /gene="ripC"
                     /locus_tag="VFI55_11185"
     CDS             1525..2685
                     /gene="ripC"
                     /locus_tag="VFI55_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan hydrolase RipC"
                     /protein_id="HET7074629.1"
                     /translation="MRLESKYPRARGLTRSALGILASLAVFSGVFAANSLADPADDAL
                     AKLSELSRQAEQTTEAMHSAQLDLNDKLAAQQAAEKKHSDDQAAANAAKAQLATFQNA
                     VNKLAAATYMGGRIDGMEAMLTAGSPQGLIDKLAVQRLMATQMRTQMASYRVAGQQAA
                     AAEQASAKSAADAKSAAEQAAAVRASLQSKQSQLQVQIAVVKSQYVTLTPQQRTALAD
                     PGQVPAGVPAPGAPAPGAPAPDALPPGAPPPGGQAPGDAQPPWFMPAPPPGGGGDRAT
                     VVQAALTQVGSAYVWGGAAPGGFDCSGLVMWAFQQAGISLPHSSQALAQGGQPVALSD
                     LQPGDVLTFYSDASHAGIYVGDGMMIHSSTYGVPVRVVPMNSSGPIYDARRY"
     gene            2738..3571
                     /locus_tag="VFI55_11190"
     CDS             2738..3571
                     /locus_tag="VFI55_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003900483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074630.1"
                     /translation="MFVLELIVAAAVRVSVERDTSTTHAQLVVPPRRAVSAPTAPMIV
                     GDRAVRLIALGGPAGERLLSRVASDIGAAVDAVEAFWGVDWPREISIVAAASDGQFAA
                     AAGGGPESQWADIAAVTVADRVDPAQRVVVGDRIVFAPAAANMSERALRIVLTHELFH
                     YAARADTALDAPRWPTEGVADFVARPSTPKPADGLSIALTLPSDADLDTPGPQRSLAY
                     DRAWWFARFVADTYGAPKLRAFYLAACGARHGDVLDAIRGVLGTDYGGVLARWKQWML
                     Q"
     gene            3675..>4146
                     /locus_tag="VFI55_11195"
     CDS             3675..>4146
                     /locus_tag="VFI55_11195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7074631.1"
                     /translation="MSRVLLVTNDFPPRRGGIQSYLSELVGRLAAGGAHSVLVYAPQW
                     KGADAFDERVASADAAVAGYRVVRHPGTLMLPEPTVDRRMRRLIADHGIDTVWFGAAA
                     PLALLAQRARQAGATRVLASTHGHEVGWSMLPVARSVLRRIGDGADVVTFVSRYT"
BASE COUNT          622 a         1405 c         1452 g          667 t
ORIGIN      
        1 cagcatccgc agctcgcgca caccggcctc cagcgaatgc gcgcactcga catggtcgac
       61 cgcgctggtc agggtcacca tctccttcat ccggccgcgc gggtcggcgc ccgtgaagta
      121 ctggcccacg cggcgccgca ggtcgaccgc ggtcccgacg tagaggacct cgcccgccgg
      181 tccgcggaac aggtagaccc ccggccggtg cggcaagccg tcggccagca ctcgtttgcg
      241 gcgctgggtc ggcgtcacgt cgggcaggta tgaacgcagg tcggcgtagg tgtgcacgcc
      301 ctggttgccg acccgctcga tgagcgcgtg caacacgtcg acggtggcgc gggcgtcgtc
      361 gagggcgcgg tgggtgggtt gcgtggccac ggcgaacagc cgcgccaagg cggccagccg
      421 cacgctgggg gcttcctcgc ggctgagcac ccggcgcgcc aggcggaccg tgcacagcac
      481 ccgcggacgg ggccaggcga tgtcggtgtg ctgggcggcg gcccggagaa acccgatgtc
      541 gaatccggcg ttgtgggcga cgagcaccgc actgccggcg aactccaaga acatcggcag
      601 cacggcgtcg atggtcgggg cgtcgcacac catcgccgtg gtgataccgg tgagctgcac
      661 gatctgaggc gggatggcgc gttgggggtc aacgagggtg gcgaactcgc cgagcacgac
      721 gccgccgcgg accttgaccg ccccgatctc ggtgatggcg tcggggggcg ctccgtcgga
      781 gcctttcgtc cgtccgccgg tggtctccag gtccaccacg acgaaggtgg tttcgcgtag
      841 cgagacttcg tcgatcgggg tggcggccgg gtcccacgat tgccccacat cggcgaaact
      901 cagctgagtc gcgccgccgg cagccatggc cgtgacgtta ggcatgtcca ccgacagctg
      961 cggagttccc acgccgggag agatgtggcg accgattgat gtcggtgccc acggttagcg
     1021 ttcgggtagc ccgactccgc ggtcggacag ccacaccgag aggacgcccg atggcaccaa
     1081 acaacgaccc agcgcacccc gaccgaggcg cgccccgagc ggacgcgccg gtggtgatcg
     1141 actgcgacga gtgcgcggtg cgtggcccgg gatgccgcga ctgtgtggtc agcgtgttgc
     1201 tcgggatccc cgagacgctg ttgggggacg aatgtgccgc tctggaagcg ctcgccgatg
     1261 ccggaatggc gccgcgatta cgcctggtgc cgattcgccg cgatcgtggg ttcggcgtcg
     1321 cttaggtgcc ttgcacacca aaaccgctga atggcctggg tttacaccct gtcactcaca
     1381 cagaaatgac agaaatctgc ccattttctt aatgccctgc tttggacaaa cgccgatatc
     1441 gtttcgtaac ctgtttgaga cctaaaccag ctcgacggcg actcgctcga ccggactcgt
     1501 cgatggccgt ttaaggatgc aattttgagg ctcgaatcta agtacccccg tgctcgtggc
     1561 cttacacgtt ctgccctggg tatattagcg agcctggcgg tcttctcagg cgtttttgcc
     1621 gcaaattccc tcgccgaccc ggccgatgac gcgctggcga aactcagcga actgtcccgc
     1681 caggccgagc agacgaccga ggcgatgcac agtgcgcagc tcgacctgaa cgacaagctg
     1741 gcagcgcagc aagccgccga aaagaagcac tcggatgacc aggccgccgc gaatgccgcc
     1801 aaggcgcagc tggccacctt ccaaaacgcg gtcaacaagc ttgcagcggc gacctacatg
     1861 ggcggccgca tcgacggcat ggaggccatg ttgaccgccg gatcgccgca aggtctgatc
     1921 gacaaactgg ctgttcaacg gctgatggca actcaaatgc gcacgcagat ggccagctac
     1981 cgcgttgccg gacagcaggc cgccgccgcc gagcaggcgt ccgccaagtc cgccgccgac
     2041 gctaagagcg cggccgagca ggccgccgcg gtccgcgcga gcctgcagtc caagcagagc
     2101 cagttgcagg tgcagatcgc cgtcgtcaag tcgcagtacg tgaccttgac gccccagcag
     2161 cggaccgcgc tcgccgaccc cgggcaggtc ccggcgggtg tcccggcgcc cggcgccccg
     2221 gcacccggtg caccggcacc cgacgcgttg ccgccgggcg cgccaccacc gggcggccaa
     2281 gcaccaggcg atgcacaacc tccctggttt atgcccgcac cgcctcccgg cggcggcggt
     2341 gaccgcgcga ccgtcgtgca ggccgcgttg acgcaggtcg gttcggctta tgtatggggc
     2401 ggcgccgcac cgggcgggtt cgactgctcc ggattggtga tgtgggcgtt ccagcaggcc
     2461 ggtatttcac tgccgcactc cagccaggcg ttggcccagg gcggtcagcc ggtggcgctg
     2521 tcggatctgc agcccggtga cgtgctcacc ttctattctg acgcgtcgca tgccggcatc
     2581 tacgtcggtg acggcatgat gattcattcc tccacctacg gcgtgccggt gcgggtggtg
     2641 ccgatgaact cgtctggccc gatctacgac gcccgccgct actaagcgcc tcggctgggg
     2701 gcggcggcac cgctggcttc cgatactgct ggcctgggtg tttgtcctcg agttgatcgt
     2761 ggccgcggcc gttcgggtca gcgtcgagcg ggatacctcc accacgcatg cgcagctggt
     2821 cgtaccgcct cgtcgggccg tcagcgcgcc cacggcgccg atgatcgtcg gcgaccgtgc
     2881 cgtccggctc atcgcgctgg gcggaccggc cggcgagcgg ctgctgtcgc gcgtggcgtc
     2941 tgacatcggc gcggcagtcg acgcggtgga ggcattctgg ggagtcgatt ggccgcgcga
     3001 gatctcgata gtggcagccg catccgacgg ccagtttgcc gccgccgcgg gcgggggccc
     3061 ggaatcgcag tgggccgata tcgcggcggt gacggtggcc gaccgcgtcg atcccgcgca
     3121 gcgagtggtg gtcggcgacc ggatcgtgtt cgcgcccgcg gcggccaaca tgagtgagcg
     3181 ggcactgcga attgtgttga cccacgagct ttttcattac gccgcacgtg ccgacaccgc
     3241 cctggacgca ccccgctggc cgaccgaagg ggtggccgat ttcgttgccc gaccgagcac
     3301 tccgaagccc gcggacgggc tgtcgatcgc cctgacgctg ccgtccgacg ccgacctcga
     3361 tacaccgggg ccgcaacggt cgctggccta cgaccgcgcg tggtggttcg cccgcttcgt
     3421 cgccgacacc tatggcgcac ccaagttgcg cgcgttctac ctggccgcct gcggcgccag
     3481 gcacggcgac gtgctcgacg ccatccgtgg cgtgctcggg accgactacg gtggcgtgct
     3541 cgcgcgctgg aagcagtgga tgctgcagta gaggtgttgc acggtctgca tcgtcgccag
     3601 gcgtcagctg gattgcccgg ctcctcgtca gaccgttgca cggtctgcat cgtcgccagg
     3661 ctagcctgac gcacgtgagc cgggtcctgc tggtaaccaa tgactttccg ccccgtcgcg
     3721 gcggaatcca gtcctacctg agcgaactcg tcggtcggct ggcagccggt ggggcgcatt
     3781 ccgtgttggt gtacgcaccg caatggaagg gcgccgacgc cttcgacgag agggtcgcct
     3841 cagccgacgc ggccgtcgcc ggttaccggg tggtgcgcca tccggggacg ctgatgctgc
     3901 ccgagccgac ggtggatcgc cggatgcggc ggctgattgc cgaccacggc atcgacaccg
     3961 tctggttcgg cgcggccgca ccgttggcgc tgctggccca gcgggcccgg caggccgggg
     4021 cgactcgggt gctggccagc acgcacggcc atgaggtggg ctggtcgatg cttcccgttg
     4081 cgcggtcggt gttgcggcgc atcggcgatg gcgccgacgt ggtgaccttc gtcagccgct
     4141 acacgc
//
LOCUS       DASSGQ010000255        10730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1263273,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000255 DASSGQ010000000
VERSION     DASSGQ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10730
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263273"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="VFI55_11200"
     CDS             <1..165
                     /locus_tag="VFI55_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase TatB"
                     /protein_id="HET7074632.1"
                     /translation="LTKHLLDGDDSFLTGNFDRPVNGAPPKPVEPSGPVARTDEQHAS
                     GPAPFDTDAT"
     gene            complement(192..1349)
                     /locus_tag="VFI55_11205"
     CDS             complement(192..1349)
                     /locus_tag="VFI55_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HET7074633.1"
                     /translation="MSSHESADSKADLSAAIRTALANVIDPELRRPITELGMVKSVDI
                     DPDGDVHVAIYLTIASCPKKSEISERVAAAVADVPGTAAVRVSLDVMSDEQRTELRKK
                     LRGDHREPVIPFAQPNSLTRVYAVASGKGGVGKSTVTVNLAAAMAARGLSVGVLDADI
                     HGHSIPRMMGSTDRPTQVESMILPPIAHEVKVISIAQFTEGNTPVVWRGPMLHRALQQ
                     FLADVYWGDLDVLLLDLPPGTGDVAISLAQLLPSAEILVVTTPQLAAAEVAERAGSIA
                     LQTRQRIVGVVENMAGLTLPDGSTLHVFGEGGGRRVAERLSRAVGADVPLLGQIPLDP
                     ALVTAGDAGIPMVLNAPDSPAGKELRSVADSLSSRRRGLAGVSLGVDPTRR"
     gene            complement(1386..2660)
                     /locus_tag="VFI55_11210"
     CDS             complement(1386..2660)
                     /locus_tag="VFI55_11210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003406276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HET7074634.1"
                     /translation="MRIGGRRGALPAAAAVRKRPLRITRTPAFGVAVITPLVFAGAVG
                     AAAPSFHGQIPPVHAHVPPVSAAITPVAAVSPTVPDLSGPVVIAIERAPTAFHIAAGA
                     SSAPPPPMIVNAPGALGIPSIALAAYRGAEQKMAVADPGCGVSWNLLAGIGRIESGHA
                     GGGAVDARGTAVVPIYGPALDGTLPGNEVIIQSSVGNRVSYARAMGPMQFLPGTWARY
                     ASDGKGDGVADPQNLFDSALAAARYLCTGGLNLRDPSQVMSAILRYNNSMAYAQNVLG
                     WAAAYATGVVPVDLPPMTGPPPPLGNAHDEHPEGLGPGLPINVIGLPMDDPFARTPLI
                     DLSQPQPAGQQPAFPWLAPNTPPAQGRMPGCTLICIGSQSPAPPGPQLPPQLVPGFGP
                     PPPDAVPPWAPPVGMPPGSPAGPPPAAPGPGG"
     gene            complement(2761..3306)
                     /locus_tag="VFI55_11215"
     CDS             complement(2761..3306)
                     /locus_tag="VFI55_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HET7074635.1"
                     /translation="MKDKSAAPRRLYTPRTSRRYSPRMDPEAVGQITESIARFFGTGR
                     YLLIQTLIVIVWIAINLFAAEARFDPYPFILLNLAFSTQAAYAAPLILLAQNRQENRD
                     RVALEQDRTRAAQTKADTEYLARELASLRLAVGEVVTREYLRHELEDLRSMLTALLPE
                     AADGEAIRASDDLERIAKKSR"
     gene            complement(3303..4610)
                     /locus_tag="VFI55_11220"
     CDS             complement(3303..4610)
                     /locus_tag="VFI55_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7074636.1"
                     /translation="MRSVNRVYVARLARMLVLGPLGESFGRVRDVVISISIVRQQPRV
                     LGLVVDLATRRSIFIPILRVAAIEPNAVTLSTGNVSLRHFEQRPGEVLAMGQVLDTMV
                     KVNDPDLPELAGLDVVITDLGIEQTRTRDWMVTRVAVRAPRRLGRRGPVNVVDWQNVQ
                     GLTPSALALPGQGVAQLLDQFEGRKAVDVADAIRGLPPKRRYEVLKALDDGRLTDVLQ
                     ELPEVDQADVLAQLGTDRSADVLEEMDPDDAADLLGVLNPTDAEMLLTRMDPDDSAPV
                     RRLLTHSPDTAGGLMTSNPVVLTPDTSVAEALARVRDPDLTTPLSSMVFVARPPTATP
                     TGRYLGCVPLQRLLREAPAELVGGIVDSDLLTLTPETPLGAVTRYLAAYNLVCGPVLD
                     DQNHLLGAVTVDDLLDHLLPHDWRADVQQQDAGAWLEGRGGSA"
     gene            complement(4696..5286)
                     /locus_tag="VFI55_11225"
     CDS             complement(4696..5286)
                     /locus_tag="VFI55_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HET7074637.1"
                     /translation="MSAPGGGFGEGARGDGVNPPPAGGREPYQPDWKPPWDPPAADYP
                     LPPYSPLGYPAEYPPSLPPPPGYGQPPGYGGPPYVPGPPQFGGPPAGYGPPPYPGGYY
                     PAPEYPGDYGHPYGPQDAARPGMNGLAIASLISSFVGLLCCIGSIVAIVLGAIALDQI
                     KRTRQDGYALAVAGIVIGVATLIVGLVIAIFAMHSR"
     gene            5446..5973
                     /locus_tag="VFI55_11230"
     CDS             5446..5973
                     /locus_tag="VFI55_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HET7074638.1"
                     /translation="MTSPFQPGQVPGASPPGAAAGRRGVPSLPTPPKGWPVGSYPTYA
                     EAQRAVDYLSEQQFPVQQVTIVGVDLMQVERVTGRLTWPKVLGGGVLSGAWLGLFIGL
                     VLGFFSPNPWAALVTGLVAGVFFGLITSAVPYAMARGTRDFSSTMQLVAGRYDVLCDP
                     QNAEKARDLLARLAI"
     gene            6001..7413
                     /locus_tag="VFI55_11235"
     CDS             6001..7413
                     /locus_tag="VFI55_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7074639.1"
                     /translation="MVSRRGRVRRIGLIVLAALTIASVSSACGSGGHEVVLSFYTPAT
                     DGETFTAVAQECTKQLGGRFAIQHVSLARAPGEQRLQLARRLSARDRGLDLMALDVVW
                     TAEFAEAGWALPLSDDPARQAEADAIVDTLPGPLATARWKHNMYGAPVTTNTQLLWYR
                     SDLVTEPPATWDGMVTEATRLHAAGQPGWIAVQANEGEGLVVWFNTLLVSAGGRVLSE
                     DGRHVTLTDTPAHRAATVNALRILKAVATAPGADPSITRTDEGTARLAVEQGKAALEV
                     NWPFVLASMLDNAVKGGVPFLPLNRDPALAGSINDLGTFAPTDEQFRTAYEASRKVFG
                     FAPYPAVTPGQPAKVTIGGLNLAVATTTRHRAEAFEAVRCLRSVQSQKKVSIEGGLPA
                     VRTSLYSDPQFQAKYPMYTVIRRQLTDAAMRPATPAYQAVSLRLSASLSPISEIDPER
                     TADELATQVQKAIDGKGLLP"
     gene            7449..8339
                     /locus_tag="VFI55_11240"
     CDS             7449..8339
                     /locus_tag="VFI55_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7074640.1"
                     /translation="MTPRAHTRRSEQRLAFVLVAPAAILMLAVTAYPIGYAVWLSLQR
                     NNLATPNDTAFIGLGNYQTILTDRYWWTALAVTLGITVVSVSIEFVLGLALALVMHHT
                     VVGKGLVRTAVLVPYAIVTVVASYSWYYAWTPGTGYLANLLPQGSAPLTEQIPSLGIV
                     VIAEVWKTTPFMSLLLLAGLALVPEDLLKAAQVDGAGFWRRLTGVILPIIKPAVVVAL
                     LFRTLDAFRIFDNIYVLTDGANNTDSVSILGYDNLFKGFNVGLGSAISVLIFLCAGVI
                     ALIYIKAFGVAAPRGDADGR"
     gene            8329..9180
                     /locus_tag="VFI55_11245"
     CDS             8329..9180
                     /locus_tag="VFI55_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7074641.1"
                     /translation="MAAKQRGAGGGPPRAPIWAVIDTFVVVYALLPVLWILSLSLKPT
                     STVKDGKLIPSSVTFDNYRGIFRGDIFTSALFNSIGIGVITTVVAVVLGAMAAYAIAR
                     LEFPGKRLLVGAGLMITMFPAISLVTPLFNIERWLGLFDTWPGLIVPYITFALPLAIY
                     TLSAFFREIPWDLEKAAKMDGATPAQAFRKVIVPLAAPGLVTAAILVFIFAWNDLLLA
                     LSLTATKAAITAPVAIVNFTGSSQFEEPTGSIAAGAIVITVPIIVFVLIFQRRIVAGL
                     TSGAVKG"
     gene            9185..10363
                     /gene="ugpC"
                     /locus_tag="VFI55_11250"
     CDS             9185..10363
                     /gene="ugpC"
                     /locus_tag="VFI55_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET7074642.1"
                     /translation="MAEIVLEHVNKSYADGATAVKDLNITIADGEFLILVGPSGCGKT
                     TTLNMIAGLEDISSGELRIGGERMNEKAPKDRDIAMVFQSYALYPHMTVRQNIAFPLT
                     LAKMKKGEIAQKVEETAKTLDLTELLDRKPSQLSGGQRQRVAMGRAIVRHPKAFLMDE
                     PLSNLDAKLRVQMRGEISRLQRRLGTTTIYVTHDQTEAMTLGDRVVVMHGGVAQQIGT
                     PDELYERPANLFVAGFIGSPSMNFFPATLTPTGLTLSFGEVVVTPEVQQVIAQHPKTE
                     NIIVGVRPEHLADAAFIDGYQRIKALTFEVKVDLVESLGADKYVYFTTAGPAVHSAQL
                     DELEAEGEVHENHFVARVPAESKAAAGQSLELALDTSKLAAFDGDSGVNLTIAPPEAE
                     "
     gene            10360..>10730
                     /locus_tag="VFI55_11255"
     CDS             10360..>10730
                     /locus_tag="VFI55_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HET7074643.1"
                     /translation="MTQVLEQVRAHVRAHFGTEPDSASVTFLGAEPIEVLRFRARANG
                     TQGIVHYVSLGCSRHPMTDPTKMVADPRRGPRAEIVLRLRDPGPVTGLARSLAVLAAT
                     PAVDGVVLEADALIDLGSPIWT"
BASE COUNT         1742 a         3520 c         3652 g         1816 t
ORIGIN      
        1 ctcactaagc acctcctgga tggcgacgac tcgttcttga ccgggaactt cgaccggccg
       61 gtcaatgggg ctccgccgaa gccggtggag ccgtcaggcc cggttgcacg caccgatgag
      121 cagcatgctt ccggaccggc gccgttcgac accgatgcga cctgaaccga ttcgaccgcg
      181 gggccggctc ctcagcgtcg cgtcgggtca actcccaatg acacgcccgc cagcccccgc
      241 cgccgcgacg acaggctgtc cgccacgctg cgcagttcct tgcccgccgg cgagtccggc
      301 gcgttgagca ccatcggaat ccctgcatca cccgcggtga ccagcgccgg gtccagcggg
      361 atttgaccga gcagcggcac gtcggcgccg acggcgcgcg acaaccgttc ggcgacccgt
      421 cggcccccgc cctcgccgaa tacgtgcagc gttgagccgt ccggcagcgt cagccccgcc
      481 atgttctcca ccacgccgac gatgcgctgg cgggtttgca gcgcgatgct gccggctcgc
      541 tcggcgacct cggccgcggc cagctgtggc gtggtcacca ccaggatctc cgcgctcggg
      601 agaagttgag ccagcgagat ggcgacgtca ccggtgcctg gcggcagatc gagcaacagc
      661 acgtccagat cgccccagta gacgtcggcc aggaactgct gcaacgcccg gtgcagcatc
      721 ggcccacgcc acaccaccgg ggtgttgccc tcggtgaact gggctatcga aatgaccttc
      781 acctcgtggg cgatgggcgg caggatcatc gactcgacct gggtgggccg gtccgtgctg
      841 cccatcatcc tggggatgga atggccgtgg atgtcggcgt cgagcacacc gaccgacagg
      901 ccgcgggcgg ccattgccgc ggccaggttg accgtgacgg tggactttcc cactccgcct
      961 ttcccggacg cgaccgcata gacccgggtc agggagttcg gctgggcgaa cgggatgacg
     1021 ggctcgcggt gatccccgcg caactttttg cgcagctcgg tacgttgctc gtcgctcatc
     1081 acgtccaggc tgacgcgcac cgccgccgtg ccgggcacgt cggcgaccgc ggcggcgacg
     1141 cgttcgctga tttcgctctt cttcgggcaa ctggcgatgg tcaggtagat cgccacgtgc
     1201 acgtcgccgt cgggatcgat atcgacgctc ttgaccatcc cgagttcggt aatgggacgc
     1261 cgcagttcgg ggtcgatcac gttcgccagt gcggtgcgga tggcggcgct caggtcggct
     1321 ttcgaatcag cggattcatg actggacatc accgccgagt gtaggcgtgt ccggtcgtcg
     1381 gctggttagc cgcccggacc gggcgctgcc ggcggcgggc cggccggcga acccggcggc
     1441 atgccaaccg ggggtgccca cggtggcacc gcgtcgggcg gcggtgggcc gaatcccggg
     1501 acaagctgtg gcggcagttg cggacccggc ggtgccgggc tctgcgagcc gatgcagatc
     1561 agcgtgcagc caggcatgcg cccctgcgct ggtggcgtgt tgggggccag ccacggaaac
     1621 gcgggctgct ggccggcggg ctgaggttgg ctcaagtcga tcagcggtgt ccgcgcaaac
     1681 ggatcgtcca tcggaagtcc gatgacgttg atcggcaggc cgggcccgag tccctccgga
     1741 tgctcgtcgt gagcgttacc gagcggtggt ggcggtccgg tcatcggcgg caggtcgacg
     1801 gggaccacgc cggtggcata cgccgcggcc caacccagca cgttctgcgc gtatgccatc
     1861 gagttgttgt aacgcaggat cgcggacatg acctgcgacg ggtcgcgcaa gttgagcccg
     1921 ccggtgcaca agtaccgcgc ggccgccagc gcggagtcga acaggttctg cggatcggcc
     1981 acgccgtcgc ctttgccgtc ggacgcgtat cgcgcccagg taccgggcaa gaactgcatg
     2041 ggccccatcg cgcgggcgta ggagacgcgg ttgccgacgc tgctctggat gatcacctcg
     2101 ttgccgggca gcgtgccgtc cagcgcgggg ccgtagatcg ggacgaccgc ggtgccgcgg
     2161 gcgtcaacgg caccgccgcc cgcgtggccg gactcgatcc gcccgatgcc ggccagcaaa
     2221 ttccagctga cgccgcagcc cgggtccgcg acggccatct tctgttcggc accgcggtat
     2281 gcggccaacg cgatcgacgg aatgccaagc gcaccggggg cattcacgat catcgggggc
     2341 gggggtgccg acgaagcccc cgcggcgatg tggaacgcgg tcggggcgcg ctcgatcgcg
     2401 atgacgacgg ggccggacag atcgggaacg gtgggagaaa cagctgccac cggcgtgatg
     2461 gcggcactca ccggcggaac gtgtgcgtgg accggcggga tttgtccgtg gaacgacggg
     2521 gccgcggcgc cgaccgcgcc ggcaaacacc aggggagtga tcaccgcaac gccgaatgcc
     2581 ggcgtccgtg ttatgcgaag cgggcgcttc cgcaccgccg cggcggccgg gagggcacct
     2641 cttcgtcccc ctatgcgcac tcgaccgtcc taaggtcagg agactatctg cttagcttcg
     2701 tgaaccagat cacaatacat aacccccgtg acgcgagtgg cgcaatggtg gaattgataa
     2761 ttagcgggat ttcttagcta tccgctccag gtcgtcactc gcccggatgg cctccccatc
     2821 ggcggcttcc ggcagcagcg cggtcagcat ggagcgcagg tcttcgagct cgtggcgcaa
     2881 gtattcgcgc gtcacgacct caccgacggc cagccgcaac gaggccagct cgcgggccag
     2941 atactcggtg tcggccttgg tctgcgcggc gcgagtgcgg tcctgttcga gtgcgacccg
     3001 gtcccggttc tcctgacggt tctgggccag caggatcagc ggcgccgcgt aggcggcctg
     3061 cgtggagaaa gccagattga gcaggatgaa cgggtacggg tcaaagcgcg cctcggccgc
     3121 gaacaggttt atcgcgatcc acacgatcac gatgagggtc tgaatcagca ggtagcggcc
     3181 ggtgccgaag aagcgggcga tggactcggt gatctggccg acggcctcgg ggtccatgcg
     3241 cggcgaatac ctgcgcgatg tccgcggggt gtacagccgt cgcggcgccg ccgatttgtc
     3301 tttcatgcgg atcctccccg tccttcgagc cacgccccag cgtcttgctg ctggacatct
     3361 gcgcgccagt cgtgcggcag cagatgatcg agcaggtcgt ccacggttac cgcccccaac
     3421 agatggttct ggtcgtcgag caccggcccg cacaccaggt tgtatgcagc gagatagcgg
     3481 gtcaccgcgc ctagcggggt ctccggcgtc agggtgagca ggtcactgtc gacgattccg
     3541 ccgacgagtt cggccggcgc ctcgcgcagc aggcgctgca gcggcacgca gcccaaatag
     3601 cggccggtgg gggtggccgt cggcgggcgc gcgacaaaga ccatcgacga cagtggggtg
     3661 gtgagatcgg gatcgcggac ccgggccagc gcctcggcga ccgacgtgtc cggggtcagc
     3721 accaccgggt tggaggtcat caagccgccc gcggtgtcgg gtgaatgggt cagcagccgg
     3781 cgcaccggag cggaatcgtc ggggtccatc cgggtcagca acatctcggc gtccgtcgga
     3841 ttgagcacac cgagcaggtc ggcggcatcg tcgggatcca tctcctctag cacgtcggcc
     3901 gatcgatcgg tgcccagctg cgccagcaca tcggcctgat ccacttcggg caactcctgc
     3961 agcacgtcgg tcagcctgcc gtcgtcgagc gccttgagca cctcgtagcg ccgcttgggc
     4021 ggcagcccgc gaatggcgtc ggccacgtcg accgctttgc gcccctcgaa ctggtcgagc
     4081 agctgtgcca ccccctgacc tggcaacgcc agcgccgacg gggtcaagcc ctggacgttc
     4141 tgccagtcca ccacgttcac ggggccgcgc cggcccagcc gtcggggggc gcggacggcc
     4201 accctggtga ccatccagtc ccgtgttcga gtttgctcga ttcccaggtc ggtgatcacc
     4261 acatcgagtc cggccagctc cgggagatcg gggtcgttga ccttgaccat ggtgtcgagt
     4321 acctggccca tcgccagcac ctcgccgggc cgctgctcga agtggcgcag ggagacgttg
     4381 ccggtgctga gcgtcaccgc attgggctcg atggccgcca ctcgcagtat cgggatgaag
     4441 atgctgcggc gggtcgccaa atcgacgacc aggcccaaga cgcgcggttg ctgccggacg
     4501 atgctgatgc tgatcacgac atcgcggacg cgcccgaagg actcgcccaa cgggcccaac
     4561 accaacatcc gcgcgagccg cgccacatac accctgttga ccgatctcat ggggaagagc
     4621 ctaggcagcc gcgggccaga gagcagatga cgagccgggc ttggtgatgg cgccaggctt
     4681 gctcatgcga tctcgctaac gcgaatgcat cgcgaagatc gcgatgacca aaccgacgat
     4741 cagggtcgcg acaccgatca ctatgccggc gacggccaat gcgtagccgt cttggcgagt
     4801 tcgcttgatc tggtcgaggg cgatcgcgcc cagcacgatg gccacgatcg agccgatgca
     4861 gcagagcagt ccgacgaacg atgaaatcag cgaagcgatc gccagtccgt tcatgccggg
     4921 ccgcgccgca tcctgcggtc cgtacgggtg tccgtagtcg ccggggtatt ccggcgccgg
     4981 gtaatagccg cccggatagg gaggcggccc gtagccggcc ggaggcccgc cgaattgcgg
     5041 cggccccggc acgtacggtg ggccgccgta gcccggtggc tgcccgtatc cggggggtgg
     5101 cggaagagac ggtgggtatt cagccggata accgagcggc gaatacggcg ggagcggata
     5161 gtcggccgcc ggcggatccc agggcggttt ccagtcaggc tgatacggct ccctgccgcc
     5221 ggccggcggg ggattgaccc cgtcgccgcg ggcgccttcg ccgaagccgc cgccgggagc
     5281 tgacatggtg tccaacctag cctatgcggc gtggccggta ccgccgcgaa ccgacggcgc
     5341 caggccggct gcggtgtttg ctcgcaggtc gccgggacgc tcgcgtcgat agggtgagag
     5401 ggccgaagat gagacgatgg ccacggcgga ggagaatgag caccaatgac tagtcctttc
     5461 cagcctggac aagttcccgg cgcatcgccc cccggcgcgg cggcgggtcg gcgcggcgtg
     5521 ccctcgttgc cgacaccgcc caagggctgg ccggtcgggt cttaccccac ctatgcagag
     5581 gcgcagcgcg ccgtcgatta cctgtccgag cagcagttcc cggtgcagca ggtcaccatc
     5641 gttggcgtcg acctgatgca ggtcgaacgg gtcaccggcc gcctgacatg gcccaaagtg
     5701 ctcggcggcg gcgtcctcag cggggcctgg ctcggcttgt tcatcgggct ggtgctcggc
     5761 ttcttcagcc ccaatccatg ggccgctctg gtcaccggtc tggtcgccgg ggtgttcttc
     5821 ggactgatca cctccgcggt tccatacgca atggcccgtg gcacaaggga tttcagctcg
     5881 actatgcagt tggtggccgg ccgttacgac gtgctctgcg atccgcaaaa cgccgagaaa
     5941 gcgcgggatc tcctggcgcg cctggcgatc tgacgcgtcg atgcgcgaga ggctgtattg
     6001 gtggtgagtc gtcgtggacg cgtgcgccga ataggcctga tcgtcctggc ggcgttgacc
     6061 atcgcttcgg tctcgtcggc ctgcgggtcc ggcggccacg aggtagtgct cagcttctac
     6121 acgccggcca ccgatggcga gaccttcacc gctgttgccc aggagtgcac caagcagctc
     6181 ggcggccgct tcgccatcca gcacgtgagt ctggccaggg ctcccgggga gcagcgcttg
     6241 cagctggccc ggcggctgag cgctcgtgac cgcggcttgg acttgatggc gctggacgtg
     6301 gtgtggaccg cggagttcgc cgaggcgggc tgggcgctgc cgctgtccga cgatccggcg
     6361 cgacaggccg aggccgacgc cattgtcgat accctgccgg gcccgctcgc gaccgcgcgc
     6421 tggaagcaca acatgtatgg cgcgcccgtg accaccaata cccaattgct ttggtaccga
     6481 tcggatttgg tgacggaacc accggcgacc tgggacggca tggtgaccga ggcgaccagg
     6541 ttgcacgccg cgggtcagcc cggctggatc gcggtgcagg ccaacgaggg cgaaggtctg
     6601 gtggtgtggt tcaacacgct gctggtgagc gcgggcggcc gtgtgctctc cgaggacggc
     6661 cgccacgtca cgttgactga cacccctgcg caccgcgccg ccaccgtcaa cgcgctgcgg
     6721 attctcaagg cggtggccac cgcccctgga gccgatccgt cgatcacccg caccgatgag
     6781 ggcaccgcgc ggttggcggt cgaacagggc aaggccgccc tcgaagtcaa ctggcccttc
     6841 gtgctggcct ccatgctgga caacgccgtg aagggcggcg tgccctttct gccgcttaac
     6901 cgcgatccgg cgctggccgg cagcatcaac gatctcggga cgttcgcgcc caccgatgag
     6961 caattccgca ccgcgtatga ggccagccgc aaggtcttcg gtttcgcgcc ctaccccgct
     7021 gtgacgccgg gccagccggc caaggtgacg atcggcgggt tgaacctggc ggtggccacc
     7081 accactcgtc atcgcgcgga ggcgttcgaa gccgtgcgtt gcctgcgcag tgtgcagagt
     7141 cagaagaagg tttcgatcga gggcggcctg cccgcggtcc gcacctcgct gtattcggat
     7201 ccgcaattcc aggccaagta tccgatgtac accgtgatcc gccggcagct caccgatgcc
     7261 gcgatgcggc cggccacgcc ggcctatcag gcggtgtcgc tgcggctgtc ggcgtccctg
     7321 agcccgatca gtgagatcga cccggagcgg acggccgacg aactcgccac gcaggtgcag
     7381 aaggccatcg acgggaaggg gctgttgccg tgaccgccct tgaaacgcat gcgccgcggc
     7441 aagcccccgt gacaccgcgc gcgcacaccc ggcggtccga acagcgactg gccttcgtcc
     7501 tggttgcacc ggcggcgatc ctgatgctgg cggtgacggc ctatccaatc gggtacgccg
     7561 tgtggcttag cctgcagcgc aacaaccttg ccacccccaa cgacaccgcg ttcatcggcc
     7621 tgggcaacta ccagacgatc ttgaccgacc gatattggtg gaccgcgtta gccgtaacgc
     7681 tgggaatcac cgtggtttcg gtgtcgatcg agttcgtgct gggcctggcg ctggcgctgg
     7741 tgatgcacca caccgtcgtc ggcaagggcc tggtgcgtac cgcggtgctc gtcccgtatg
     7801 ccatcgtcac cgtcgtcgcg tcgtacagct ggtactacgc ctggacgccg gggacgggtt
     7861 acctggccaa cctgctgccg cagggcagcg cgccgctgac cgaacagatc ccgtcgctgg
     7921 gcatcgtcgt catcgccgag gtctggaaga ccacaccgtt catgtcgctc ttgctgctgg
     7981 ccggattggc gctggtacca gaggatctgt tgaaagccgc acaagtcgac ggggccggct
     8041 tctggcgccg gttgaccggg gtcatcttgc cgatcatcaa gcctgcggtc gtggtcgctc
     8101 tgctattccg gacgttggac gcattccgca tcttcgacaa catctacgtg ctgaccgacg
     8161 gcgccaacaa caccgactcg gtgtcaatcc tgggctacga caacctgttc aaggggttca
     8221 atgtcgggtt gggctcggcg atcagcgtgt tgatcttcct ctgtgcgggc gtcatcgcgc
     8281 tcatctacat caaggcgttc ggggtcgcgg cgcccagagg tgacgccgat ggccgctaaa
     8341 caacgaggcg cagggggagg cccaccgcgc gccccgatct gggccgtcat cgacactttc
     8401 gtcgtggtct atgcgttact tccggtgctg tggatcctca gcctgtcgct caaaccgacg
     8461 tcaaccgtga aggacggcaa gctgattccg tcgtcggtga ccttcgacaa ctaccgtggc
     8521 atcttccggg gcgacatctt cacctccgcc ctgttcaatt ccatcggcat cggcgtgatc
     8581 accactgtgg tcgcggtggt gctcggcgcg atggcggcct acgcgatcgc gcggctggaa
     8641 tttcccggca agcgcctgct tgtcggtgcc ggcctgatga tcaccatgtt cccggcgatc
     8701 tcactggtca ccccgttgtt caacatcgaa cgctggctcg gtctcttcga cacctggccc
     8761 ggtctgatcg tgccctacat cacttttgcg ttgccccttg ccatctacac actgtcggct
     8821 tttttccggg agatcccctg ggacctggag aaggccgcca aaatggacgg cgctacgccg
     8881 gcgcaggcct tccgcaaggt gattgtcccg ctggcggcgc ccggactggt gaccgcggcg
     8941 atcctggtgt tcatcttcgc gtggaacgac ctgctgttgg cgctgtccct gaccgccacc
     9001 aaggcggcga tcaccgcccc cgtggcgatc gtgaacttca ctggcagttc gcagttcgag
     9061 gagccaaccg gatcgatcgc ggccggtgcc atcgtgatca cggtcccgat catcgttttt
     9121 gttctaatct tccaacgacg gattgtcgcc gggttgactt ctggcgctgt gaagggatag
     9181 cgcgatggcc gagattgtgt tggagcacgt caacaagagt tacgccgacg gcgcgaccgc
     9241 ggtgaaggac ctgaacatca ctatcgcgga cggcgaattc ctgatcctgg tggggccttc
     9301 cggctgcggc aagaccacga cgctgaatat gattgccggg cttgaggata tctcgtcggg
     9361 tgagctgcgc atcggtggtg agcggatgaa tgagaaggcg cccaaggacc gcgatatcgc
     9421 aatggtgttc cagtcgtacg cgctttaccc gcacatgacc gtgcggcaga acatcgcgtt
     9481 cccgctgacc ctggcgaaga tgaagaaggg cgagatcgcg cagaaggtcg aggagacggc
     9541 caaaaccctt gacctgaccg aacttttgga ccgcaagccg tcccagttgt cgggtgggca
     9601 acgacagcgg gttgcgatgg gccgggcgat cgtgcggcat cccaaggcat tcttgatgga
     9661 cgagccgctg tccaacctgg atgccaaact tcgggtgcag atgcgcggcg aaatttcgcg
     9721 tctgcaaaga agattgggca ccaccaccat ctatgtcacc cacgatcaaa cagaggccat
     9781 gacgctggga gatcgagtgg tggtgatgca cggtggtgtg gcgcagcaga tcggcactcc
     9841 cgacgagctc tacgagcgtc ccgccaatct gttcgtcgcg gggttcatcg gctcgccgtc
     9901 gatgaatttc ttccccgcga ccctgacccc gaccggcctg acattgtcct tcggcgaggt
     9961 ggtggtgacg ccggaagtcc agcaggtgat cgcgcagcac ccgaaaaccg agaacatcat
    10021 cgtcggggtg cgacccgaac acctggccga cgccgcattc atcgatggat atcagcgcat
    10081 caaagccctg acgttcgagg tgaaggtcga cttggtggaa tctttgggcg ccgacaaata
    10141 cgtgtacttc accaccgcgg gtccggccgt ccactcggcc cagttggacg agctggaggc
    10201 ggagggggag gtacacgaaa accatttcgt cgcaagggtt cctgccgagt caaaggcggc
    10261 cgccgggcag tcgctcgagt tggctttgga cacttcgaaa ctggccgcgt tcgacggtga
    10321 ctccggagtg aatctgacca tcgcgccgcc cgaggctgag tgacacaggt tctggaacag
    10381 gtgcgcgcgc acgtgcgcgc ccacttcggc accgagccgg attccgcgag cgtgacgttc
    10441 ttgggcgccg agcccatcga ggtgctgcga tttcgcgcgc gggcgaacgg aacgcagggg
    10501 atagtgcact acgtgtccct ggggtgctcg cgccacccga tgaccgatcc gaccaagatg
    10561 gtcgccgacc cccgacgtgg cccgcgcgcc gaaattgttc tgcgcctgcg agatcccggg
    10621 cccgtcaccg gccttgcccg cagcctggcg gtgctggcgg caacgccggc tgtcgatggc
    10681 gtggtgctgg aagccgacgc gctgatcgac ctcggctcgc cgatctggac
//
LOCUS       DASSGQ010000256         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_625257,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000256 DASSGQ010000000
VERSION     DASSGQ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625257"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2266
                     /locus_tag="VFI55_11260"
     CDS             <1..>2266
                     /locus_tag="VFI55_11260"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET7074644.1"
                     /translation="VPVSYDTNTKTNAANRDYAVPSFLALRADQPFGVVSSGFVGTPS
                     DGLAQLDSAHILTPTDSALGGNVEQTAQVRFDTHGDAMLALGFGTTQADAVAAASATA
                     GRPFGSLRGQYRSGWLSYDDALNTPSGSLPGLTPAQQAAAVNEYYVSANVVKASEDKT
                     FPGAIVASIASPWGQAVSAGDPANTYFGSYREVFSRDLYEAWTGLLTDGDLATARAAT
                     RFLLEKQQLPDGSLPRNSLVNGKLAPDSFNTQLDEVAYPILMVLQSGLAGDAGLWPHV
                     QAAANYLIAHGPSFGPERWEEQSGYSPSTIAAEIAGLVAAATIAQRHASPADARIWLA
                     TADQYQRSIKGWAVTTNGPLSSQPYFIRLSKTGDPNAAISYNVGNGGPTLDQRAVIDA
                     GFLELVRLGELSATDPDVLNSLTVVDKTIEKQSASGSGWLRYNGDGYGDCHILPGHTC
                     PLEGRPWAPSNMGTGHPWPVLSAERGEHDLVTGQATSAGQLLAAMGAMSSGVGLVPEQ
                     VWDAPNVAASPFGTDPTIASIGFVNGKPDGSAAPLTWGAGSQVRLTADLTAGRLLEQP
                     AQTVDRYITHTQLATTLTVTSPPDQTAATNPIHVAGTAVPGATIDVADVGTGTTTHAG
                     GTAAADGSFSFNIAPLIGNNVLVVTSTAPNGGTAQAVRTVVNDVVQGTLLFDATDPTG
                     DDNGPGNYAYPTSSDFHAGAFDLTDFQVYDTGTTVTFRVQTRDLTPTFGSPLGAQLVD
                     VYVHEPGASPTSTAA"
BASE COUNT          377 a          817 c          718 g          354 t
ORIGIN      
        1 cggtgccggt ctcctacgac acgaacacca agacaaacgc cgccaatcgc gattatgccg
       61 tgccgagctt cctggccctg cgggccgacc aaccgttcgg cgtagtgagc agcggcttcg
      121 tcggcacccc gtcggatgga ctcgcccagc tcgactccgc gcatatcctg acgccgaccg
      181 attccgctct cggcggcaat gtggagcaga cggcgcaggt gcgcttcgac acgcacggcg
      241 acgcgatgct cgccctcggt ttcggcacca cgcaggccga cgccgtcgcc gccgccagcg
      301 ccacggccgg ccggcccttc gggtcgctgc gcggccaata ccggtcgggg tggctttcat
      361 acgacgacgc gctcaatacc ccatctggaa gtctccccgg ccttacgccg gcccagcagg
      421 cggctgcagt gaatgagtac tacgtgtccg ccaacgtagt caaagcgagc gaggacaaga
      481 cattcccagg ggcaatcgtg gcctcgatcg cctcgccgtg ggggcaggcg gtctccgccg
      541 gcgacccggc caacacctac ttcggctcct accgggaagt cttctcccgg gacctctatg
      601 aggcatggac ggggctgttg acggacggcg atctggccac cgcccgcgcc gccacgaggt
      661 tcctgctcga gaagcagcag ctccccgacg ggtcgttgcc acgcaactcg ttggtcaacg
      721 gcaagcttgc gccggattcc ttcaataccc aactggatga ggttgcctat ccgatcctga
      781 tggtgttgca gtccggcctg gccggcgacg ccggactgtg gccgcacgta caggcggcgg
      841 cgaactattt gatcgcgcac gggccgtcgt tcgggcccga gcgttgggag gagcagagcg
      901 gatactcgcc gtcaacaatc gcggccgaga tcgccgggct ggtcgccgcc gcaacgatcg
      961 cccagcggca cgcttctcct gccgacgccc ggatctggct cgcgaccgcg gaccagtacc
     1021 agcgatccat caagggctgg gccgtcacga cgaacggccc tttgtcgagt cagccgtact
     1081 tcatccggct ttccaagacc ggtgatccga atgcggcgat cagctacaac gtcggcaatg
     1141 gcggcccgac gctcgaccag cgcgcggtca tcgatgccgg attcctcgag ctggttcgcc
     1201 tcggcgagct gtcggccacc gacccggacg ttctgaactc gctgacggtg gtggacaaga
     1261 ccatcgagaa gcaatcggcg agcggctcgg ggtggttgcg ctacaacggc gacgggtatg
     1321 gcgactgcca tatcctgccg ggccacacgt gcccgctcga aggtcggccg tgggcgccgt
     1381 cgaacatggg caccgggcac ccctggccag tgctctctgc cgagcgcggc gagcatgacc
     1441 tggtcaccgg gcaggcgaca tcggcaggcc agctgctcgc ggcgatgggc gctatgtcct
     1501 ccggggtcgg cttggtaccc gagcaggtgt gggacgcgcc taacgttgcc gcgtcgccct
     1561 tcggcactga cccgacgatt gcgtcgatcg gcttcgtcaa cggcaagccc gacggctccg
     1621 ctgcaccttt gacctggggt gccggctcgc aggtccgact gacggccgat ctcaccgcgg
     1681 gacgcctgct cgagcagcct gcccagaccg tcgaccgcta tatcacccac acccagctgg
     1741 cgaccacgct caccgtgacc tcgccgcctg accagacggc ggccaccaac ccgattcatg
     1801 tcgcgggaac ggccgtgccc ggcgcgacca tcgatgtagc ggacgtaggc acggggacga
     1861 ccacgcacgc cggtggcacc gctgccgccg atgggtcatt cagcttcaac atcgcgccgc
     1921 tgatcggcaa caacgtcctt gtggtcacca gcacggcgcc caacggcggt acggcccagg
     1981 ccgttcggac ggtcgtcaac gacgtcgtcc aaggcacgct gctctttgac gccacggacc
     2041 ccaccggcga cgacaacgga cccggcaatt acgcctatcc gacgtcctcg gacttccacg
     2101 ccggcgcgtt cgacctgacc gatttccagg tctacgacac cggcactacc gtgaccttcc
     2161 gagtccagac ccgcgacctg acgccgacct tcggcagccc gctcggcgcg caactcgtcg
     2221 acgtctacgt gcacgagccg ggtgcgtcgc cgacgtcgac agcggc
//
LOCUS       DASSGQ010000257         6653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1142232,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000257 DASSGQ010000000
VERSION     DASSGQ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6653
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142232"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(882..1961)
                     /gene="trpD"
                     /locus_tag="VFI55_11265"
     CDS             complement(882..1961)
                     /gene="trpD"
                     /locus_tag="VFI55_11265"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET7074645.1"
                     /translation="MAVSPEAPSPARAPSWRQVLGRLTDGQDLTRGQAAWAMGQIMTG
                     DASPAQIAAFAVAMKMKVPTAAEVSELADVMLSHARQMPADAVRDDTVDIVGTGGDGV
                     NTVNLSTMASIVVAAAGVPVVKHGNRASSSLSGGADTLEALGVRIDLEPDQVARSLAD
                     VGIGFCFAPLFHPSYRQASAVRREIGVPTVFNLLGPLTNPARPRAGLIGCAFADLAEV
                     MAGVFAARRSSVLVVHGDDGLDELTTTTTSTIWRVQAGTVDRLTFDPAGFGFARAELD
                     ELVGGDAQANAAEARAVLAGAKGPVRDAVVLNAAGAIVAHAGLSSRAEWLPAWEIGLE
                     RAGAAIDSGAAEQLLARWVRFSQQV"
     gene            2101..2712
                     /locus_tag="VFI55_11270"
     CDS             2101..2712
                     /locus_tag="VFI55_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HET7074646.1"
                     /translation="MTSAVGTSGTAITSRIHSLNRPNMVSVGTIVWLSSELMFFAGLF
                     AMYFTARAQAGGKWPPPPTELNLYQAVPVTLVLIASSFTCQMGVFAAERGDVFGLRRW
                     YVITFLMGLFFVLGQAYEYYHLMSHGTTIPGSAYGTVFYLATGFHGLHVTGGLIAFIF
                     LLARTAMTKFTPAQATASIVVSYYWHFVDIVWIALFTVIYFIR"
     gene            2771..3619
                     /locus_tag="VFI55_11275"
     CDS             2771..3619
                     /locus_tag="VFI55_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7074647.1"
                     /translation="MRFGRRPGRARKGQSDRSRRRLRRRLSGGLLLLIALTVAGGLAA
                     VLTPTPQVAVADESSAALLRTGKQLFDTSCVSCHGANLQGVPDHGPSLIGVGEAAVYF
                     QVSSGRMPAMRGEAQAPRKESIFDESQTDAIGAYVQANGGGPTVVRNPDGSIAMHSLR
                     GEDLGRGGDLFRLNCSSCHNFTGKGGALSSGKYAPDLEPANEQQILTAMLTGPQNMPK
                     FSDRQLSFEAKKDIIGYLKAVTDERQPGGYGLGGFGPAPEGMAMWIIGMVAAIGLALW
                     IGARAS"
     gene            3616..4791
                     /locus_tag="VFI55_11280"
     CDS             3616..4791
                     /locus_tag="VFI55_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HET7074648.1"
                     /translation="MSDDAHGAQQPDEAALATMSQQELVALGGKIDGVETVFKEPRWP
                     VEGTRAEKRAERGVALWLLLGGGFGLALLLIFLFWPWQYKPKEAAGSFLYTLTTPLYG
                     VTFGLSILSIAIGAVLFQKRFIPEEISIQERHDGASREIDRKTVVANLTDAYQGSTIG
                     RRKIIGLSLGMGLGAFGLSSLVAFVGGMIKNPWKPVVPTADGMKAVLWTSGWTPRYHG
                     ETIFLARATGATEGAPFVKLRPEDMDAGGMETVFPWRESDGDGTTVESHEKLSAIKMG
                     IRNPVMLIRIRPPDMTRVVKRQGQESFNFGEFFAFTKVCSHLGCPSSLYEEQTYRILC
                     PCHQSQFDALHFAKPIFGPAARALAQLPITIDTNGYLVANGDFAEPVGPAFWERTTS"
     gene            4788..6446
                     /locus_tag="VFI55_11285"
     CDS             4788..6446
                     /locus_tag="VFI55_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HET7074649.1"
                     /translation="MSPKFSPPKIGDVLAKQGEDIDTRYHPSAAVRRQLNKVFPTHWS
                     FLLGEIALYSFIVLLLTGVYLTLFFDPSMTEVTYNGVYQPLRGVEMSRAYQSALDISF
                     EIRGGMFVRQVHHWAALMFSASIMVHLARIFFTGAFRRPREANWIIGSLLLILSMFEG
                     YFGYSLPDDLLSGLGLRAALSSISLGMPIIGTWLHWALFGGDFPGTILIPRLYALHIL
                     LLPGIILALIGTHLALVWFQKHTQFPGPGRTERNVVGVRVMPVFAVKSGAFFAAIVGV
                     LGLMGGLLQINPIWNLGPYKPSQVSAGSQPDFYMMWTEGLARIWPPWEFYFWHHTIPA
                     PVWVALIMGLVFILLIIYPFLEKRFSGDYAHHNLLQRPRDVPVRTSIGAMAIAFYMLL
                     TLAAMNDIIAFKFHISLNATTWIGRIGMVILPPIVFFISYRWCIGLQRSDRAVLEHGV
                     ETGIIKRLPQGAYIELHQPLGPVDAHGHPIPLEYEGAPVPKRMNKLGSGGTPGSGSFL
                     FADPPSEDAALRHAEHASEQRALTALRDYQDTIVGSPNGEHSEH"
BASE COUNT         1037 a         2309 c         2187 g         1120 t
ORIGIN      
        1 tgcggatgct ggccgcgcac gccccgccgt acaaccgcag gtccaggttc ccccagcggt
       61 ggtggtggct gaccctgacc gacgagccgt ttccgcggct gttggcggtg cgggcaccgc
      121 ggcacgaacg cgccgtcggc ccgtttcggt cccgctccga cgccgccgac acggcgacgc
      181 tgctcgcgcg cttcaccggg ctgcggacct gcaccgcgcg gctgggacgt tcggccctgc
      241 acgggccggg ctgccccgag gccgtggtgt cgccgtgccc ggcggaccgt gacgtcgcgg
      301 ccactcagta cgcggcggcc gcggcccgcg tcgcggcgtt gatcgacggg ctggacaacg
      361 gcgctttggc cgcggccgtg gaccaggtca ccctgctcgc cgagcgacgt catttcgaga
      421 gtgccgcgcg actgcgcgac cgcaccgccg ccgccgtcga gacgctctgg cgcgggcaac
      481 ggctgcgcgc gctggccgcc ctacccgagc tgatcgccgc cgcgccggcc ggccctgacg
      541 gtcagggcgg ctaccacctc gccgtcatcc ggtacggcca gctcgccgcc gccggcaccg
      601 cccggcgtgg agtgccgccg atgccggtgg tgaacgccct tcacgccgga gcccagaccg
      661 tcctgcagac gccggccccg ctcggcggcg cgctggtcga ggaaaccgcg ctcatcgcgc
      721 gctggctggc cggccccgga gtgcgcatcg tgcgggtcgc agacccggac tccccgggct
      781 gggcctcgcc gctgcgatcc gccggagcct gggccggatg ggcggccgcg gcgcgctcgg
      841 cgcgcctcgc gagtgagcag gcttggtccg atggtcgtga ctcaaacctg ctgactgaac
      901 cgcacccatc gcgcgagcaa ctgttcggcc gcacccgagt cgatggccgc gccggcgcgc
      961 tccagcccga tctcccacgc cggcaaccat tcagcgcggc tggataggcc ggcatgggcc
     1021 acgatcgctc cggcggcatt gagcacgacg gcgtcgcgca ccgggccctt ggcgccggcc
     1081 agcaccgcac gcgcctccgc cgcgttggct tgcgcgtcgc cacccaccag ctcgtcgagt
     1141 tcggcgcggg cgaacccgaa tccggccgga tcgaacgtca accggtccac cgtgccggcc
     1201 tgcacccgcc agatcgtgct cgtggtggtg gtcgtcaact cgtcgagccc gtcgtcgccg
     1261 tgcaccacca gcacgctgga cctgcgcgcc gcgaacaccc ccgccatcac ctcggcgagg
     1321 tcggcgaacg cgcagccgat caaacccgcc cggggccggg ccggattggt aagcggccca
     1381 aggagattga acacagtggg caccccgatt tcgcggcgca ccgccgacgc ctgccggtac
     1441 gacgggtgga acagcggcgc gaagcagaac ccgatgccga catcggcgag gctgcgggcg
     1501 acctggtcgg gttcgagatc gatgcgcacc ccgagggcct ccagcgtgtc agccccgccg
     1561 gacagcgacg acgacgcccg gttgccgtgt ttgaccaccg gcaccccggc cgccgccacc
     1621 acgatcgagg ccatggtgga caggttgacg gtgttgacgc cgtccccgcc cgtgccgacg
     1681 atgtcgacgg tgtcgtcacg gaccgcgtcg gcgggcatct ggcgcgcgtg gctcagcatg
     1741 acgtcggcca gctcgctgac ctcggccgcg gtgggaacct tcatcttcat cgcgaccgcg
     1801 aaggcggcga tctgggccgg gctcgcatcg ccggtcatga tctggcccat ggcccaggcg
     1861 gcctgacctc gcgtcagatc ctgaccgtcg gtcagccggc ccaggacctg ccgccacgac
     1921 ggcgcacgcg cgggcgaagg ggcttcgggt gacacggcca cgcgccgatg gtcccacgag
     1981 caccaaccgt cgccgaaaat cggacgaagc cggtgccacg gcgacgccga caagacgcgc
     2041 ccaacgccaa ttgccgcccc gggtagagtt ggacaactac aaagcgtcat acttgcggat
     2101 gtgaccagtg ctgtggggac ctcaggtacc gcgatcacgt cgcgaattca ttcgctgaat
     2161 aggccaaaca tggtcagtgt cgggaccata gtctggcttt ccagcgagct gatgttcttt
     2221 gctggcctgt tcgcgatgta cttcacggca cgcgctcagg ccggtggcaa gtggccgccg
     2281 ccgccgaccg agctgaatct ctaccaagcc gtgccggtga ccctggtgct gatcgcgtcg
     2341 tcgttcacct gccagatggg ggtgttcgcg gccgagcgcg gcgacgtctt cgggctgcgc
     2401 cgctggtacg tgatcacgtt cctgatgggc ctgttcttcg tcctcggcca ggcctacgag
     2461 tactaccacc tgatgtcgca cggcacgacc atccccggca gtgcctacgg caccgtgttc
     2521 tacctggcca ccggctttca cggactgcac gtcactggtg gtctgatcgc attcattttc
     2581 ctgctggccc gcaccgcgat gaccaagttc accccggcgc aggcaacggc cagcatcgtc
     2641 gtgtcctact actggcattt cgtcgacatc gtgtggatcg cgctgttcac cgtgatctat
     2701 ttcattcgat gaaggccggc gctttcaaaa cggagtgaat aggagtgcac ggttgaagaa
     2761 cctggggtcc atccgattcg gcaggcgacc gggccgggcg cgaaaagggc agagcgatcg
     2821 ctcgagacgg cgtcttcgcc ggcgattgtc gggtggcttg ctgctgctga tcgcgctgac
     2881 cgtcgccggc gggctggccg ccgtcctgac ccccacccca caggtggcgg ttgccgacga
     2941 atcgtcggcg gcgctgctgc gcaccggcaa gcagctgttc gacacctcct gcgtgtcgtg
     3001 ccacggcgct aatctgcagg gtgtgcccga ccacgggccg agtctgatcg gcgtcggcga
     3061 ggcggccgtc tacttccagg tgtccagcgg ccggatgccc gccatgcgcg gtgaggccca
     3121 ggcgccgcgc aaagagtcga ttttcgatga atcccagacc gacgcgatcg gcgcctacgt
     3181 ccaggccaac ggcggcgggc ccaccgttgt gcgcaacccc gacggcagta tcgcgatgca
     3241 ctcgctgcgc ggcgaggacc tgggccgcgg cggtgacctg ttccggctca actgctcctc
     3301 gtgccacaac ttcaccggaa agggcggggc cctgtcgtcg ggcaagtacg cacccgacct
     3361 ggagcccgcc aatgagcagc aaatcctcac ggcgatgctg accggcccgc agaacatgcc
     3421 gaagttctcc gaccgccagc tctccttcga ggccaagaag gacatcatcg gttacctcaa
     3481 ggccgtcacc gacgagcgcc agccgggtgg ctacggtctc ggcggattcg gaccggcacc
     3541 cgagggcatg gccatgtgga tcatcgggat ggtggcggcc atcgggttgg cactgtggat
     3601 tggggcgcga gcatcatgag cgacgacgcg cacggcgcgc agcagcccga cgaggcggcg
     3661 ctggccacga tgtcccagca ggaactggtg gcgctgggcg gcaaaatcga cggcgtcgag
     3721 accgttttca aggagcctcg ctggccggtc gagggcacca gggccgagaa gcgcgccgag
     3781 cgcggggtgg cgctctggct gttgctgggc ggtggattcg ggctggcgct gctgctgatc
     3841 ttcctgttct ggccgtggca gtacaagccg aaggaggccg ccggtagctt cctgtacacg
     3901 ctgaccaccc cgctgtacgg cgtgaccttc gggctgtcca ttctgtcgat cgcgatcggc
     3961 gcggtcctgt tccagaagcg gttcatcccc gaggaaatct cgattcagga gcgacacgac
     4021 ggcgcgtctc gcgagatcga ccgcaagacc gtcgtggcaa acctgaccga cgcctaccag
     4081 ggttcgacga tcgggcggcg caagatcatc gggctgtcac tgggcatggg cttgggcgcc
     4141 ttcggccttt ccagcctggt ggcctttgtg ggtgggatga tcaagaaccc gtggaagccg
     4201 gtcgtcccga ccgccgacgg gatgaaggcc gtgctgtgga cctccggctg gaccccgcgc
     4261 taccacggcg agacgatttt cctggctcgg gccaccggcg cgaccgaggg ggcgccgttc
     4321 gtcaaattgc gcccggaaga catggacgcc ggcggaatgg agacggtctt cccgtggcgg
     4381 gagtccgacg gcgacggcac caccgtggaa tcccacgaaa agctcagtgc aatcaagatg
     4441 ggcattcgca acccggtgat gctgatccgt atccggcccc ccgacatgac gcgggtggtc
     4501 aaacgccagg ggcaggagag cttcaacttc ggcgagttct tcgccttcac caaggtctgc
     4561 tcgcatctgg gttgcccgtc atcgctgtat gaagagcaga cctaccgaat tctgtgccct
     4621 tgtcatcagt cgcagttcga cgcgttgcat ttcgccaagc cgatattcgg tccggcggcc
     4681 cgcgcattgg cgcaactgcc catcacgatc gacaccaacg ggtatctggt cgccaacggt
     4741 gacttcgccg aacccgtcgg accggcattc tgggagcgca ccacatcatg agtccgaaat
     4801 tctctcctcc gaagatcggt gatgtcctgg ccaaacaagg cgaggacatc gacacgcggt
     4861 atcacccctc ggcggcagtg cgcagacagc tcaacaaggt cttccccacg cactggtcgt
     4921 tcctgctcgg tgaaatcgcg ttgtacagct tcatcgtgct gctactcacc ggcgtgtatt
     4981 tgacgctgtt cttcgacccg tcgatgaccg aggtgaccta caacggcgtc taccagccgt
     5041 tgcgtggagt cgagatgtcg cgcgcctacc aatcggcgct ggacatctcc ttcgagatcc
     5101 gcggcggcat gttcgtccgg caggtccacc actgggccgc gttgatgttc tccgcgtcga
     5161 tcatggtgca cctggcacgc atcttcttca ccggtgcgtt ccggcggccc cgtgaggcga
     5221 actggatcat cggctcgctg ctgctgatcc tgtccatgtt cgagggctac ttcggttatt
     5281 cgctgcccga cgacctgctg tcgggactcg gcctgcgggc cgcgttgtcg tcgatctcgc
     5341 tgggcatgcc gatcatcggc acctggctgc actgggcgct gttcggcggc gatttccccg
     5401 gcaccatcct gatcccccgg ctctacgcgc tgcacatttt gctgctgccg gggatcatcc
     5461 tggccctcat cgggacgcac ctggccttgg tgtggttcca gaagcacacc cagttccccg
     5521 gccccggccg caccgaacgc aacgtcgtcg gcgtgcgagt gatgccggtg ttcgccgtca
     5581 agtccggcgc attcttcgcg gccatcgtcg gcgtgctggg cctgatgggc ggtctgctac
     5641 agatcaaccc gatctggaac ctcggtccct acaagccatc tcaggtgtcg gccggttcgc
     5701 agcccgactt ctacatgatg tggacggagg gcctggcccg tatctggccg ccgtgggagt
     5761 tctacttctg gcaccacacc attcccgccc cggtctgggt cgcgctgatc atgggcctgg
     5821 tgttcatcct gctgatcatc tacccgttcc tggagaagcg attctccggc gactacgcgc
     5881 accacaattt gctgcagcgg ccgcgagatg ttccggtgcg cacctcgatc ggcgccatgg
     5941 cgatcgcctt ctacatgctg ctcaccctgg cggcgatgaa cgacatcatc gcgttcaagt
     6001 tccacatctc gctgaacgcg acgacctgga tcggccgcat cgggatggtc atccttccgc
     6061 cgatcgtgtt cttcatctcc taccggtggt gcatcgggct gcagcgcagc gaccgggcgg
     6121 tgctcgaaca cggcgtcgag acggggatca tcaagcggct accgcagggc gcctacatcg
     6181 agctgcatca gccgctcggt cccgtcgacg cccacggtca cccgatacca ctcgagtacg
     6241 aaggcgcgcc ggtgcccaag cggatgaaca agctgggatc cggtggaaca ccgggtagcg
     6301 gcagcttctt gttcgccgat ccgccgtccg aggatgcagc gctgcgccac gcggagcacg
     6361 cctcagagca gcgtgccctg accgcgcttc gcgactacca ggacaccatc gtcggttcgc
     6421 ccaacggcga gcacagcgag cactagcccg ccttcacccg gcgacgatgc gggccgccgc
     6481 gcggcccgtg gtggggtccc ccagccgccc tgggcagcga gggggcgagg cggaaccgct
     6541 actcgcgcgg gactttacgt gacggacaaa acgccaatcc cgcgcgcggt acggctaagc
     6601 ggccacgcgc gcggagacca cactgcgtag ctggcgaata tgcagccact cga
//
LOCUS       DASSGQ010000258        37855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_534781,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000258 DASSGQ010000000
VERSION     DASSGQ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 37855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37855
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534781"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..496)
                     /locus_tag="VFI55_11290"
     CDS             complement(119..496)
                     /locus_tag="VFI55_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074650.1"
                     /translation="MAVSGASHAYLYVHGYQHIPTIGTAFLIQASVAFALAVLILAGG
                     PWWLEWAAAATAGGSLVAFVLSRTVGLFGFTERGWDPSPHAAITVIAEVLCVLLWAGA
                     LFGSRRAQESKLIVPEPLGVYER"
     gene            complement(538..1467)
                     /locus_tag="VFI55_11295"
     CDS             complement(538..1467)
                     /locus_tag="VFI55_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7074651.1"
                     /translation="MTQDPHTMNRRQLIRHTAWFGAAVGLAVAGGEVISHVAGSASAE
                     HAQGRPTLRFAQVSDSHIGFTGGANPDVTKTFDHAIDQINNLGYTPDFVIHTGDLTHL
                     SSPDQFDQVKQMLSGLRTPHVFTVPGEHDSVDDAGQKYRSAFGAGTRGDGWYSFDIAG
                     VHVIALVNTLNLKKLGHLGIDQLEFVKKDVAGLSSDTPIIVFSHIPLFAMYPDWGWGT
                     DDATQALSQLSRFSSVTCLNGHVHQLFSKTEGNVTFYSGTTTAYPLPHPGDGPAPKPV
                     TLPAGKLGEALGIREVSYTKGQTALALKEQRLL"
     gene            complement(1517..1969)
                     /locus_tag="VFI55_11300"
     CDS             complement(1517..1969)
                     /locus_tag="VFI55_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016893366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HET7074652.1"
                     /translation="MPRKHLAVLAVGALLLAGCSASRHDAQPSITFGSAGGTPGMSGM
                     SGMPGMPSAPAGTSSATDSPVAVAGDAVTIDNFAFAPATLTVRAGSTVTWTNRDEEPH
                     TVAAADGSFHSPGMGTGATYSHTFPSAGKFDYVCSIHPSMHGTVVVTQ"
     gene            complement(2009..2713)
                     /locus_tag="VFI55_11305"
     CDS             complement(2009..2713)
                     /locus_tag="VFI55_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET7074653.1"
                     /translation="MNARGLRRYVDDLLGGRRPKPFAPDDFEAAQIRTAIELRAARQG
                     DDAPRQEFLTDLHRRLAEQMHGTTPKAGPRQSATRRQVIVGTSAAAAAAVAAVAIDRA
                     VIGGPGEAPGGDAVAGQLTPNDGSWQRVAGSADVPDATMHPFDLGSVTGFVRRVDGRV
                     EAVSGVCTHQGCRLWFDAPDDTLRCPCHTTSFSPSGQVLTHQLPIAPKPLPTLMVREA
                     NGSIEVFAPPREDEPA"
     gene            complement(2710..3234)
                     /locus_tag="VFI55_11310"
     CDS             complement(2710..3234)
                     /locus_tag="VFI55_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7074654.1"
                     /translation="MRAVPDSGYDAGFSGYPDWEAVYQDNAAWVYRTLFARVGNRADA
                     EDLTAEVFLAALRPLRLSASVGEVRAYLRATARTVLAAHWRETLGREITSIDDIEQPP
                     ESEEAISTAPQRVAQVLDRLPDRYRQILEMRFLQGNSIKESAAELGISVANAKVLQHR
                     ALRLAAQVNEGGQP"
     gene            complement(3315..4739)
                     /locus_tag="VFI55_11315"
     CDS             complement(3315..4739)
                     /locus_tag="VFI55_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HET7074655.1"
                     /translation="MRTIEADYLVVGAGAMGMAFVDTLVSETEARVVLVDRNHQPGGH
                     WTMAYPFVRLHQPSAFYGVNSLRLGSDTIDQVGCNVGLYELATAGEICAYYDRVMRQH
                     LLPTGRVSYFPMSEYRGDGRFATLAGTDYVVNVARRVVDATYLRVMVPSMRPPPYRVA
                     AGVECVAPNDLPRRPAHERYVVVGAGKTGIDTCLWLLGQGIAPDRLTWIMPRDSWLLD
                     RAMVQPGPLFADLIKANFAARVRAIADAASVEDLFCRLEDAGALLRIDPAIRPSMYRC
                     ATVTQAELEQLRAITDVVRMGHLLRTDADQMILDGGTVPIHGRALFLDCTADGAEKRP
                     ATPIFDDGRITLQSVRGCQQVFSAALVAHVEATHLNDPDDSVKNRLCVPLPHPDTDLD
                     WLRITLADYGNQLRWLDDPDLTAWLAAARLDVLGHLLGHLQAPGSANPRVRDRILSMA
                     KSALSATAGKLDRLMVEEPALTAP"
     gene            4832..6352
                     /locus_tag="VFI55_11320"
     CDS             4832..6352
                     /locus_tag="VFI55_11320"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET7074656.1"
                     /translation="MTTQIPHFIDGKRTAGQSNRTADVFNPSTGRVQAKVPMAGQADV
                     DAAVASAAEAQKGWAAWNPQRRARVMMRFIELVDEAGDELAELLSLEHGKTLADARGD
                     IQRGIEVIEFCVGIPHLLKGEYSEGAGAGIDVYSLRQPLGVVAGITPFNFPAMIPLWK
                     AGPALACGNAFLLKPSERDPSVPVRLAELFIEAGLPPGVFQVVHGDKEAVDAILHHPD
                     IKAVGFVGSSDIAQYIYSTSAATGKRSQCFGGAKNHMIVMPDADLEQAVDALIGAGYG
                     SAGERCMAISVAVPVGEQTAERLRARLVERINHLRVGHSLDPKADYGPLVTEAALTRV
                     RDYIDQGVAAGAEIVVDGRDRRSDDLAFGDANLEGGFFIGPTLFDHVTPDMSIYTDEI
                     FGPVLCMVRAHDYEEALRLPSEHEYGNGVAVFTRDGDTARDFVSRVQVGMVGVNVPIP
                     VPVAYHTFGGWKRSGFGDLNQHGPASIQFYTKVKTVTSRWPSGIKDGAEFVIPTMN"
     gene            6373..7548
                     /locus_tag="VFI55_11325"
     CDS             6373..7548
                     /locus_tag="VFI55_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7074657.1"
                     /translation="MDLFMLNDDERVITETAAAFAAKRVAPHALEWDATQHFPVDVLR
                     EAAELGMAAIYCRDDVGGSGLRRLDGVRIFEQLAIADPVTAAFLSIHNMCAWMIDTFG
                     TDEQRKEWVPRLAPMDAIASYCLTEPGAGSDASALTTRAVKQGGDYVLDGVKQFISGA
                     GASDVYVVMARTGGQEKPGPRGISAFIIEKDTPGLSFGAHEQKMGWHAQPTAQVILDG
                     VRVPADAMLGGAEGEGAGFGIAMNGLNGGRLNIAACSLGGALAAFDKAGAYVRERHAF
                     GSSLLDEPTIRFALADMATGLETSRMMLWRAANALDTDDPDKVELCAMAKRYVTDTCF
                     EVADKALQLHGGYGYLREYGLEKIVRDLRVHRILEGTNEIMRVVIGRAEAARFRATL"
     gene            7559..8443
                     /gene="mmsB"
                     /locus_tag="VFI55_11330"
     CDS             7559..8443
                     /gene="mmsB"
                     /locus_tag="VFI55_11330"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HET7074658.1"
                     /translation="MTELFQVAFLGLGNMGGPMSANLVTAGHTVRGFDPVPAAVAAAA
                     AGGVAVFDSAADAVSEADVVITMLPNGDVVKRCYAEIMPGARAGALFIDSSTISVNDA
                     REVHALAESHGLSQLDAPVSGGVKGAVAGTLAFMVGGDESALQRARPVLEPMAGKIIH
                     CGAAGAGQAAKVCNNMVLAVQQIVIGEAFVLAENLGLSAQSLFDVITGATGNCWAVHT
                     NCPVPGPVPTSPANNDFKPGFATALMNKDLGLAMDAVASTCSAAPLGTHAAEIYAKFA
                     ADHGALDFSAVIEALRSS"
     gene            complement(8469..9017)
                     /locus_tag="VFI55_11335"
     CDS             complement(8469..9017)
                     /locus_tag="VFI55_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HET7074659.1"
                     /translation="MDPLVAHQRAQDTFAGVLANVSPDQFDGPTPCSEWTIRDLIEHV
                     IGGNEYVGIWSASNQQPTARPDDTAAAHRASAAAAQEIFAAPGGMDGIFKLPFGEFPA
                     QLFVGIRTTDVLTHAWDLAAATGQSTDLDPELAAEQLAAARAFVGAQFRGTGKLFAEE
                     QPCSSERAPADQLAAFLGRKVP"
     gene            complement(9062..9568)
                     /locus_tag="VFI55_11340"
     CDS             complement(9062..9568)
                     /locus_tag="VFI55_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7074660.1"
                     /translation="MAAARPQKRDPIAAARANWERAGWAEVSQGMVAVTSVMRAHQIL
                     LARVETALRPYDLSFSRFELLRLLAFSRTGALPITKASDRLQVHVTSVTHAIRRLEAD
                     GLVQRLPHPTDGRTTLVQITDLGRATVDDATVTLNQQVFADVGMDAGESEALVSSIES
                     LRRNAGDF"
     gene            complement(9586..10638)
                     /locus_tag="VFI55_11345"
     CDS             complement(9586..10638)
                     /locus_tag="VFI55_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7074661.1"
                     /translation="MQPVPSPFAPEIDDALPTAVDPLALSLNENPFPPLPAVRSALIR
                     SICAANRYPEFLPERLRSLIAHHIDLPSDQVVLGAGATGVVLQALLALTTPGDTIAMA
                     SPTFDGYSIVARMGRLNAVTVPLDEDGHNDLDGLADAAANARVVVVCRPHNPTGTLEP
                     AAELVRFLRRVPRDTIVLLDEAYVEFVAPEHRVDVAALVTRFPNVVVVRTFSKAYGLA
                     GLRIGYGLASPELARELWSQQLPFGIAITSMLAVAASYRAEDQLLQRIQSINAERRYL
                     RMRLSALGIYTTDAHANFIYLPSRGGRGRSWHEVFADGGLHVRCYADGGARITVGNRG
                     STLAVLAAMGKATPGS"
     gene            complement(10638..11657)
                     /locus_tag="VFI55_11350"
     CDS             complement(10638..11657)
                     /locus_tag="VFI55_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HET7074662.1"
                     /translation="MSKPSVSLIDVSTYLPGEPIPAEYYAQFAESDDLRDNLMFRAPK
                     FRHHVGSDESAIDMVERAAQGLIERHGHDAVENADVLITHTQVPDMAFYGEGGGIAHR
                     LGLRPSWVLDLNNGGCAAFVLALNVARKLLSTGEGHTALIAIAQNAAGQFFDQPTIRR
                     KAQSAVPGDGAAVGLVTASDESPILDVECRTYGAYAGEMTLSYDPPRKWWAAGPGEGS
                     IGFTESKITKVLARGNRQVPEVALAVCDRIGLPAKDIDLLVTNQPNRAFLRNWREALE
                     LPPERHRDTFEECGNLFGAGIPVNLDRAISDGQVKAGDVVMMAAFAHAGDFAGAAAVR
                     WGGRG"
     gene            complement(11676..12323)
                     /locus_tag="VFI55_11355"
     CDS             complement(11676..12323)
                     /locus_tag="VFI55_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7074663.1"
                     /translation="MSLPALEDIRTPIEGVVRIETSPREKATPIIMDMMRSVYPHDQV
                     FGEYCTVNDYIDCPPDELYEYLADTRSLEEWTYSLRGFTTTDEPGLWLAYDRLGSETK
                     IYTRTIANSHARTVDYHCAWDQGKHLWMIYLMRVIDAQVVLDKPGSVVLWTNCRHPFY
                     DRNPYPETAPPQRPVWVGDFWDMFGAGHLLEMKNLKAIAEYRHRNGLPVTPVWMR"
     gene            complement(12304..14058)
                     /locus_tag="VFI55_11360"
     CDS             complement(12304..14058)
                     /locus_tag="VFI55_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET7074664.1"
                     /translation="MPRTHRVVDHIVERLAAIGVDHIFGVDGANIEDLYDAAHFRSDI
                     TAVLAKHEFSAATMADGYSRSGAGLGVVAATSGGGALNLVAALGESLTSRVPVLALVG
                     QTATTMDGRGSFQDTSGDNGSLNAQAVFSAVSLFCERVLTPAGVVPALSAAIAAARTG
                     GPAVLLLPKDIQQGRVDRERAGTRSGLATQGLRPIGNPQPIVGILRHARGPVTLIVGE
                     QVARDNARAELEALRAVLRARVATVPDAKDVAGTPGLGSSSALGVTGVMGHPGVAQAV
                     ADSAVCLVVGTRLSVTARTGLDDALAAARTVSIGSAPPYVSCTHVHTDDLRGSLRMLT
                     QALSGAGRPTGVRVPDVVRRTELTPPPCLGAGVRYRDAMAVLDGCVPDGADIVVDAGN
                     TGAAAIHYLPARRGGRFAVALGMGGMGYSFGAGIGMAFGRPNSGRPGGRTVVIAGDGA
                     FFMHGMEVHTAVHYQLPVTFVLFNNNAHAMCVTRERLFYEDLYSYNRFRSSRLGAGLA
                     AMFPGLTSVDVTDLEGFAAAMRGVLAVDGPAVVSVECAADEIPPFAPFLNTQPAKADL
                     TQDSSIAKENRTDVAASA"
     gene            complement(14108..14884)
                     /locus_tag="VFI55_11365"
     CDS             complement(14108..14884)
                     /locus_tag="VFI55_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET7074665.1"
                     /translation="MDEMRTPLRGIGPPYDNHGNEVFDLTGRDNHRYAMWDAAYVLGS
                     LSASDRREFEEHMANCPACREAVADMSGVPALLSRLDRAEVAAINGRAESLPETPALS
                     PELLPSLLATVRWRRRRTRVATWVASAAAAAVLGIGVFVGVQGYTSAPAQQVTASSQP
                     MVQVGTNLLASTVQLSSQHWGTSINLKCVCLAPLNAHHDTLAMVVVGRDGSQTRLATW
                     VAEPGHTATPAGSISTPVDQIAAVQVVSADSGEVLLQRSM"
     gene            complement(14955..15491)
                     /locus_tag="VFI55_11370"
     CDS             complement(14955..15491)
                     /locus_tag="VFI55_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7074666.1"
                     /translation="MASTRGASGAAEAALMKALYDEHAAVLWRYALRLTGDASQAEDV
                     VQETLLRAWQHPEVIGDTERSARAWLFTVARNMIIDDRRSARYRNVVGSLDEAGAPEQ
                     STPDEVNAALDRLLIADALVQLSPEHRAVIERSYYRGWTTAQIAADLEIAEGTVKSRL
                     HYAVRALRLTLQELGVTR"
     gene            complement(15578..16378)
                     /gene="map"
                     /locus_tag="VFI55_11375"
     CDS             complement(15578..16378)
                     /gene="map"
                     /locus_tag="VFI55_11375"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET7074667.1"
                     /translation="MSPLARLRSRRVVPQRSPGELDAMAAAGAVVAAALHTVHAAAVS
                     GTSTLGLDEIAESVIREAGATPSFLGYHGYPASICASVNDRVVHGIPSAAEILAPGDL
                     VSIDCGAVLDGWHGDAAITFGVGALDAADEALGDATRESLEAGIAAMVAGNRLSDVAH
                     AIEMGTRAAEARYGRSFGIVEGYGGHGIGRHMHMDPFLPNEGAPGRGPLLAPGSVLAI
                     EPMLTLGTSKTVVLDDQWTVTTADGSRAAHWEHTVAVTDGGPRILTLG"
     gene            complement(16381..16938)
                     /locus_tag="VFI55_11380"
     CDS             complement(16381..16938)
                     /locus_tag="VFI55_11380"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET7074668.1"
                     /translation="MRVVLLGPPGAGKGTQAVKLAEKLEIPQISTGELFRRNIANGTT
                     LGLEAKRYLAAGDLVPSDLTNQLVDDRLNDPDTADGFILDGYPRSLEQAKALHEMLGR
                     RGTDIDAVVEFRVSQDELFARLKARGRADDTNDVILNRMKVYRDETAPLLEYYGDQLK
                     DQLKTVDAVGSMDEVFARALEALGK"
     gene            complement(16935..18260)
                     /gene="secY"
                     /locus_tag="VFI55_11385"
     CDS             complement(16935..18260)
                     /gene="secY"
                     /locus_tag="VFI55_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET7074669.1"
                     /translation="MLSAFISSLRTVDLRRKILFTLGIVLLYRVGAALPSPGVNYPNV
                     QQCIKEASGGEAGQIYSLINLFSGGALLKLTVFAVGVMPYITASIIVQLLTVVIPRFE
                     ELRKEGQSGQAKMTQYTRYLAIALAILQATSIVALAANGGLLQGCSLDIIADQSIFTL
                     VVIVLVMTAGAALVMWMGELITERGIGNGMSLLIFVGIAARIPAEGKSILDSRGGIIF
                     AAVCVAALIIIVGVVFVEQGQRRIPVQYAKRMVGRRMYGGTSTYLPLKVNQAGVIPVI
                     FASSLIYIPHLITQLVRSGTGGVGNSWWDKFVGTYLSDPSDPVYIAIYFGLIIFFTYF
                     YVSVTFNPDERADEMKKFGGFIPGIRPGKPTADYLRYVLNRITLPGSIYLGVIAVLPN
                     LFLQIGNGGGVQNLPFGGTAVLIMIGVGLDTVKQIESQLMQRNYEGFLK"
     gene            18755..19660
                     /locus_tag="VFI55_11390"
     CDS             18755..19660
                     /locus_tag="VFI55_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7074670.1"
                     /translation="MTRTDQDSWDLASSVGATATMVAAARALASSETDAIISDPFAAP
                     LVRAVGVDFFIRLVDGAVLPADAPEGQRDLRLETDSIAVRTRFFDDFFLNAARDGIKQ
                     SVILAAGLDARAYRLAWPPGSAVYEVDQPKVIEFKDAAMANLSAAPSADRRTVSIDLR
                     EDWPAALRRSGFDVTQPTSWSAEGLLMYLPPDAQDRLFDKITELSAPGSKLATEYHGD
                     SGETMTARAQQFNDRWANLGCDINLSGLFFDGERSNVVDYLTERGWQVTTRPRRDYFA
                     DYGRVFPEDETSQLRNIVALTAVRI"
     gene            19671..20585
                     /locus_tag="VFI55_11395"
     CDS             19671..20585
                     /locus_tag="VFI55_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7074671.1"
                     /translation="MTDTKRFEGDSWDLASSVGATATAVAARRAMASKGPNPLIDDPF
                     AEPLVNAVGVDAFIRMMNGEIEVAEDDPAFTAQRLSEGMAVRTRFFDSFFLSAAEAGV
                     RQAVILASGLDTRAYRLPWPAGTVVYEIDQPQVIEFKTRTLADLGATPTADRRAVGID
                     LRDDWPAALRASGLGPTRPTAWIAEGLLGYLPPDAQDRLFDNITALSAPGSRIGTGYV
                     PDIGDRVQKRGRELSERWRRMGLNLNWSELVYADERNDVVAYLSDRGWQTTVSSTPEM
                     YARNGFEFPTEPSLVAFGDIKYVSATLT"
     gene            20607..21536
                     /locus_tag="VFI55_11400"
     CDS             20607..21536
                     /locus_tag="VFI55_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7074672.1"
                     /translation="MARTDQDTWDLASSVGATATWVAAARALASRRPDALIDDPFADP
                     LVRAVGKEFFVRVLDGEITDESGGLDPEADPDAEFNLQRMVNMMAVRTRYFDDFFTDA
                     AAAGVRQAVILAAGLDSRPYRLAWPAGTVTYEVDQPAVIAFKSTTLSNLGAEPTAERR
                     TVSVDLRDDWLTALRDKGFDDSIPSAWSAEGLLMYLPPEAQDRLFDAITTLSAPGSWL
                     ATEYHRGGMPLLERRAKAMARRWQPYGFDVDVSTLVYFGERTPAAEYLSARGWQVSTC
                     TRADMFAAAGLPIPRGDSLESLQNSIAVTAVLR"
     gene            complement(21540..22205)
                     /locus_tag="VFI55_11405"
     CDS             complement(21540..22205)
                     /locus_tag="VFI55_11405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7074673.1"
                     /translation="MTDQDRKAARREIADALLKALERRHEVLDLMVESEDKAAAVEAI
                     GALLETSHVGAEAVMGMSFDQLTKDSRRKIQSELEDLNKQLSFTLKERPASSGDTLEL
                     RPFSAEEDRDIFAARTEDMGAAGDGSGGPAGNLDDEIRAALARVDDEEATWLVAVDSG
                     QKVGMVFGELVHGEVDVRIWIHPDYRKKGYGTAALRRSRSEMAWCFPAVPMVVRSPAV
                     DPR"
     gene            complement(22198..23580)
                     /locus_tag="VFI55_11410"
     CDS             complement(22198..23580)
                     /locus_tag="VFI55_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET7074674.1"
                     /translation="MSREDVTIGIDIGRTAVKAVAADADGRVTARVRIPHELRVPAPD
                     RLEHDAGRAWRRGPLAALDQLGCPNAHAVAVSAMAPSLTAVDSAGRPLTPGLLYGDGR
                     GRAPSVPGQPAPLLPALGEATEFLRWTVGQAPDAAGYWPAPAVANHALAGEAVIDFAT
                     AATAFPLFDGTGWNPAACAERGATVEQMPRAETIGAAAGRVHGTPAVLGIGTIDALCE
                     QMVAGADHDGDVLVLCGTTLIVWTTIPQARQVPGLWTIPHVAPGKSQIGGASNAGGLF
                     LGWVDRVVGPGDPTAVDPRRVPVWSPYIRGERTPLHDPDRRAMLAGLDLTHDAASLRR
                     AAYEASGFVVRQLIELSGAAVSRVVAAGGGIRIRPWLQAIADATGRPVQVSGVAEGAA
                     LGAAFLGRIAAGLETSIADAARWVSIECTVDPDPAWAAAIEDRYQRFLELGNRGSRDT
                     PGGVLGLCND"
     gene            23611..24597
                     /locus_tag="VFI55_11415"
     CDS             23611..24597
                     /locus_tag="VFI55_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7074675.1"
                     /translation="MTSRPRALVTAPLRGPGFAKLQQLADVVYDPWIGELGATLRIYN
                     GEQLAERIAAESADVVVVESDSVRGPVFDLGLRAIAATRGDPNNVDVEGATAAGIPVL
                     NTPARNADAVAELAVALLLATTRHVVTADADVRSGNMFRDGSIPYQRFRGWEIAGRTA
                     GLVGLGAVGRALRWRLSGLGMRVIAHDPYNDEARHGLDELLGESDVVSLHAPVTDDTV
                     GMIGAGQFAAMRDGAVFLNTARAQLHDTDALVHALRGGKLAAAGLDHFAGEWLPIDHP
                     LVGMPNVVLTPHIGGATWNTEARQAQMVADDLEALLSGKTPAHIVNPEVLAS"
     gene            24594..25250
                     /locus_tag="VFI55_11420"
     CDS             24594..25250
                     /locus_tag="VFI55_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HET7074676.1"
                     /translation="MKFVDSPETAVLDAAKDMLRRGLVEGTAGNISARRSDGNIVITP
                     SSVDYRDMQLDDLVLVDPDGAVLQAAAGRSPSSESKLHLACYHAFDDIGSVIHSHPVW
                     ATMFAIAHQPIPACIDEFAVYCGGDVRCTEYAASGTPEVGANAVKALDGRAAALIANH
                     GLVAVGPRPDKVLHVTALVERTAQIVWGARALGGPVPIPEDVNRNFAMVYSYLRANPL
                     "
     gene            complement(25342..26574)
                     /locus_tag="VFI55_11425"
     CDS             complement(25342..26574)
                     /locus_tag="VFI55_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7074677.1"
                     /translation="MKALVYGVPPEPYAIPDGANTLTKNLARTPTALREVPDPRLLHR
                     DWVITRPRLTGICGSDSKQILMDFGEGDADNAMAAFCSFPQVMGHEVVADVVEVGPEA
                     RGLQEGQRVVLNPWLSCGPRGVEPPCPACENGDYSLCWSFTDGDIKPGIHTGVSADVT
                     GGYAELMPAHDSMLFAVPESIPDELAVFADPFSVSLHAITRHPPPRAGRVLVYGAGSL
                     GLCAVAILRALYPDVAVAVVARFGAQAELARRFGASLVLAHEPSLAVIEDVVAWGGGR
                     LRRPLLGLPMAYPGAVEVVYDTIGKPETFEVGVRVLCARGTLVKAGVHAPGRWEWSPL
                     YFKEISWVGSNAFGIEEVEGQRKHAIAHYLDLVAAGRVDLRPMLTHTFGLEQWRDAFL
                     AIANQGRSGAVKVAIDQR"
     gene            complement(26571..26723)
                     /locus_tag="VFI55_11430"
     CDS             complement(26571..26723)
                     /locus_tag="VFI55_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074678.1"
                     /translation="MAYTAAGKRVLSTGASPGLGAALGGDEFEHYVPDTQEVVDAANA
                     GLRAPR"
     gene            26823..27779
                     /locus_tag="VFI55_11435"
     CDS             26823..27779
                     /locus_tag="VFI55_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7074679.1"
                     /translation="MTSTRYEGDTWDLASSVGVTATMVAAARAMASRVDRPLIDDPYA
                     EPLVRAVGVDLLTRLASGELDPDDLNDVHDGATGSAGAMSRMADNMAVRTKFFDEFFL
                     GATNAGIKQAVILASGLDARAYRLPWPAGTVVYEVDQPQVIEFKSRTLAELGAAPTAE
                     RRAVAIDLREDWPAALRAAGFDPAQPTAWSAEGLLGYLPPEAQDRLLDTITELSAPGS
                     RLATESAPNPEPGDEEKMRERMQAISERWRAHGFDLDMAGLVYFGERNEAAPYLSGHG
                     WQLTSASIRELFADNGLPPFEDDDMRMGEMLYTSGTLNSNAK"
     gene            27797..28717
                     /locus_tag="VFI55_11440"
     CDS             27797..28717
                     /locus_tag="VFI55_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7074680.1"
                     /translation="MDSGRTDGDSWGPASSVGATATMVAASRAVASQGPNALLDDPLA
                     DPLVRAVGLDPFVRIIDGEIDFEDDPLFNRKARTEQITVRTRFFDDFFIGATQDGVRQ
                     AVILASGLDTRAYRLRWPEGTVVYEIDQPQVIAFKTNTLAGIGANPTADRRAIAIDLR
                     DDWPAALRQGGFDVTQPTAWSAEGLLPYLPPEAQDRLFDNITALSAPGSRLATEHVPD
                     PNAFTDERLQRISERWQRFGFNLNAADLFYPGERNVVVDYLGTHGWAVTAHPARELYA
                     QNGFEFPEDEMAATFGELSYVAAILKQGVG"
     gene            28727..29638
                     /locus_tag="VFI55_11445"
     CDS             28727..29638
                     /locus_tag="VFI55_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7074681.1"
                     /translation="MARAHDDNWDLASSVGATATMVAAGRAMATKDPRGLIDDPFAEP
                     LVRAVGIDFFTRMIDGELDLDAIENLTPVRMQAIVDGMAVRTKYFDDYFADATGSGVQ
                     QVVILASGLDSRAYRLAWPPGTVVYEIDQPQVIEFKTTTLDGIGAQPTAVRRTVAIDL
                     RADWPAALRAAGLDTTAPTAWLAEGLLIYLPPEAQDRLFDNITALSVPGSTIATEFGP
                     GIIDFDADRVRQMSGAFRDQGIDIDMASLVYAGERNHVVDYLGAKGWDAQGVTRTELF
                     KRHHIDVPAPEQDDPLGEIIFISGRLA"
     gene            complement(29646..31433)
                     /gene="sppA"
                     /locus_tag="VFI55_11450"
     CDS             complement(29646..31433)
                     /gene="sppA"
                     /locus_tag="VFI55_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET7074682.1"
                     /translation="MFAFLPSIPGLLDRVDDVRALVGRVDTARHHGVPNGCVLEFSLR
                     SAPPETTGLDPLAIITGGSRPMALRDVVAAIHRAAEDPRVAGLIARVQLAASPPAVVQ
                     ELREAIAAFSAVKPSLAWAETYPGTLSYYLASAFGEVWMQPSGSVGLIGFASNATFLR
                     DALAKAGIEAQFVARGEYKSAANLFTEDGFTDAHREAVTRMLESLQEQVWQAVAQSRK
                     IDPAALDELADRAPLLRDDAVASGLVDRIGFRDEAYARIAELVGVEDVSDEESPPRLY
                     LARYAGAARARLAPPMPPVPGRRTKPTLAVVTIDGAIVNGRGGPQFLPLGTSNVGGDT
                     IAAALREAAADDSVSAIVLRVDSPGGSVTASETIWREVKRARERGKPVVASMGSVAAS
                     GGYYVSMGADAIVADPGTITGSIGVITGKLVVRDLKDRLGVGSDTVRTNANADAWSVD
                     APFTPQQRAHREAEADLFYTDFVTRAAEGRKLTTEAVEVVARGRVWTGADALARGLVD
                     ELGGFRTAVRRAKVLAGLDADTEVRIINYPGSSLLDFVRPRTSSRPAAASLPDALGAL
                     LARSVAGILDNVEQTLSGASVLWLGNSRL"
     gene            31468..32394
                     /locus_tag="VFI55_11455"
     CDS             31468..32394
                     /locus_tag="VFI55_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7074683.1"
                     /translation="MRFSIAIPQLDADTFDGPGLRSYLVRAEELGFEGGWVLEQIIGA
                     APLLAPLELLAYCAACTERLRLGVAVLVTSLHEPLQVAASVTAVDRLSHGRLDVGVAP
                     GGGARNFAAFGVDKDTFISYFTEGLELMKAAWSDEPRVTFHGRFRDVDDLPVQPKPVQ
                     RPHPPIWFGGLAPKALARAVRLGDSFLGAGSSTTEAFAGAVAVVRRELDEQQKDPTHF
                     TIGKRVYLMVDDDAPRARERVLTGLHRIYGEMRGIDAVPVFGTPDDVARGLREVIDAG
                     AEMLLLNPVGADVPENREQMELLAAEVIPQLV"
     gene            complement(32488..32928)
                     /gene="rplO"
                     /locus_tag="VFI55_11460"
     CDS             complement(32488..32928)
                     /gene="rplO"
                     /locus_tag="VFI55_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET7074684.1"
                     /translation="MTIKLHDLKPAPGSKTARTRVGRGEGSKGKTAGRGTKGTKARKG
                     VPVTFEGGQMPIHMRLPKLKGFRNRFRTEYEIVNVGDIERLFPQGGVIGVGDLVAKGA
                     VRKNSLVKVLGDGELTVKVDVSAHKFSGSAREKITAAGGSATEL"
     gene            complement(32925..33131)
                     /gene="rpmD"
                     /locus_tag="VFI55_11465"
     CDS             complement(32925..33131)
                     /gene="rpmD"
                     /locus_tag="VFI55_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET7074685.1"
                     /translation="MSQLKITQVRGTIGARWKQRESLRTLGLRRIRHSVIREDNPQTR
                     GLIAVVNHLVVVEPAEAAAGGNTK"
     gene            complement(33128..33808)
                     /gene="rpsE"
                     /locus_tag="VFI55_11470"
     CDS             complement(33128..33808)
                     /gene="rpsE"
                     /locus_tag="VFI55_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET7074686.1"
                     /translation="MAEQSAGGQGQDTRGDGRGDRDSRGRRDGGGGGRGRDRDGEKSN
                     YLERVVAINRVSKVVKGGRRFSFTALVIVGDGNGMVGVGYGKAKEVPAAIAKGVEEAR
                     KGFFRVPLIGGTITHPVQGEAAAGVVLLRPASPGTGVIAGGAARAVLECAGVHDILAK
                     SLGSDNAINVVHATVAALKLLQRPEEVAARRGLPIEDVAPAGMLKARRESEALASASA
                     TREGTPQP"
     gene            complement(33831..34199)
                     /gene="rplR"
                     /locus_tag="VFI55_11475"
     CDS             complement(33831..34199)
                     /gene="rplR"
                     /locus_tag="VFI55_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET7074687.1"
                     /translation="MGQSASATRRVSRLRRHSRLRKKIAGTSQRPRLVVHRSARHIHV
                     QLVNDENGTTVAAASSIETDVRGLQGDKKARSVRVGQLIAERAKAAGVDSAVFDRGGY
                     TYGGRIAALADAARENGLNF"
     gene            complement(34241..34780)
                     /gene="rplF"
                     /locus_tag="VFI55_11480"
     CDS             complement(34241..34780)
                     /gene="rplF"
                     /locus_tag="VFI55_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET7074688.1"
                     /translation="MSRIGKQPVPVPAGVDVTIEGQNVSVKGPKGTLELTVAEPIRVA
                     RSDDGAIVVTRPDDERHNRSLHGLSRTLVSNLVTGVTQGYTTKMEIFGVGYRVQLKGS
                     NLEFALGYSHPVVIEAPDGITFAVQSPTKFTISGIDKQQVGQISANIRRLRRPDPYKG
                     KGVRYEGEQIRRKVGKTGK"
     gene            complement(34793..35191)
                     /gene="rpsH"
                     /locus_tag="VFI55_11485"
     CDS             complement(34793..35191)
                     /gene="rpsH"
                     /locus_tag="VFI55_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003974254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET7074689.1"
                     /translation="MTMTDPIADFLTRLRNANSAYHDEVTLPHSKIKANIAQILKAEG
                     YITDYRTEDARVGKALIVQLKYGPSRERSIAGLRRVSKPGLRVYAKSTNLPRVLGGLG
                     VAIISTSSGLLTDRQAARQGVGGEVLAYVW"
     gene            complement(35371..35556)
                     /locus_tag="VFI55_11490"
     CDS             complement(35371..35556)
                     /locus_tag="VFI55_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HET7074690.1"
                     /translation="MAKKALINKANRKPRFAVRAYTRCSKCGRPRAVYRKFGLCRICL
                     REMAHAGELPGVQKSSW"
     gene            complement(35560..36156)
                     /gene="rplE"
                     /locus_tag="VFI55_11495"
     CDS             complement(35560..36156)
                     /gene="rplE"
                     /locus_tag="VFI55_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET7074691.1"
                     /translation="MTTTESSERPVKVQPRLKERYRNEIRDALHKQFGYGNVMQIPGV
                     TKVVVNMGVGEAARDAKLINGAVNDLALITGQRPEIRKARKSIAQFKLREGMPIGVRV
                     TLRGDRMWEFLDRLTSIALPRIRDFRGLSPKQFDGVGNYTFGLAEQSVFHEIDVDKID
                     RVRGMDINVVTSATTDDEGRALLRALGFPFKEEPQRGS"
     gene            complement(36161..36478)
                     /gene="rplX"
                     /locus_tag="VFI55_11500"
     CDS             complement(36161..36478)
                     /gene="rplX"
                     /locus_tag="VFI55_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET7074692.1"
                     /translation="MKIKKDDTVLVIAGKDKGAKGKVLKVYPERNRVLVQGVNAIKKH
                     TPVSTTQRGAQSGGIVTQEAPIHASNVMVVDSDGKPTRVGYRVDEETGKRVRISKRNG
                     KDI"
     gene            complement(36479..36847)
                     /gene="rplN"
                     /locus_tag="VFI55_11505"
     CDS             complement(36479..36847)
                     /gene="rplN"
                     /locus_tag="VFI55_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET7074693.1"
                     /translation="MIQQESRLKVADNTGAKEILCIRVLGGSSRRYAGIGDIIVATVK
                     EAIPGGNVKRGDVVKAVVVRTVKERRRPDGSYIKFDENAAVIIKPDNDPRGTRIFGPV
                     GRELREKRFMKIISLAPEVL"
     gene            complement(37116..37586)
                     /locus_tag="VFI55_11510"
     CDS             complement(37116..37586)
                     /locus_tag="VFI55_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015292931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HET7074694.1"
                     /translation="MHVSPLMGGVCRGVVVASTVLGVAVISAGAAAADGPAQLRSRLG
                     DVCLDAPSGGWYAAVVINPCNGSDFQRWNLNGRQLESVAFPGNCLTIPGDAWFSHLGP
                     CIDTYAQHWSIQPNGQVTTDTGGCLAVLGGPGPGTWVSTRWCNGDPGQGWDSVP"
     gene            complement(37753..>37855)
                     /locus_tag="VFI55_11515"
     CDS             complement(37753..>37855)
                     /locus_tag="VFI55_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4436 family protein"
                     /protein_id="HET7074695.1"
                     /translation="AGSWIDQAVVIWVLIALATALVLYIAAWYRHSN"
BASE COUNT         6293 a        12839 c        12429 g         6294 t
ORIGIN      
        1 ccagcggcgg gacatttttg tacacgacga cggtcggttc gcagcagacg cctgtcgtgt
       61 caagtcagct gggaggcgac ttcaataccg ggatcattcc gttcctggag gatccgatct
      121 acctctcgta cacccccagc ggttccggga cgatgagctt cgattcctga gccctcctgg
      181 aaccgaacaa agcacccgcc cacagcagca cgcatagcac ctcggcgatc acggtgattg
      241 cggcatgcgg cgaagggtcc catccgcgct cggtgaagcc gaagagcccc acggtcctgg
      301 acaggacgaa cgccaccagc gatccgcccg ccgtcgcggc tgccgcccat tcgagccacc
      361 agggaccgcc ggcgaggatc aggacagcca acgcgaacgc gacactggcc tggatgagga
      421 atgccgtgcc gatcgtcgga atgtgttggt aaccatggac atacaggtag gcgtggctgg
      481 cgccgctgac ggccaaggat gccgccagac ccagccggag caagaattcc ttcaatttca
      541 cagcagcctc tgctctttca atgccagggc ggtctgccct ttggtgtagc tgacctcgcg
      601 aatgcccagg gcttcgccga gcttgccggc cggtagcgtg accggcttgg gtgccggtcc
      661 gtcacccgga tggggtagcg gataggcggt tgtcgtgccg ctgtagaacg tcacgttgcc
      721 ttccgtcttg gaaaacaatt ggtgcacatg cccgttgagg caggtaaccg atgagaagcg
      781 gctcagctgg ctcagcgctt gggtggcgtc atcggtgccc cagccccagt ccgggtacat
      841 cgcgaacaac gggatgtggc tgaacacgat gatgggggtg tcgctcgaca atccggcgac
      901 gtcctttttg acgaactcca actggtcgat acccaaatgc ccgagcttct tgaggttcag
      961 cgtgttcacc aacgcgatca cgtgcacccc ggcgatgtcg aagctgtacc acccgtcgcc
     1021 gcgcgttccc gccccgaagg cgctgcggta cttctgcccg gcatcgtcga cagaatcgtg
     1081 ttcgccgggc accgtgaaga catgcggggt tcgcagtccg ctcagcatct gcttcacttg
     1141 gtcgaactga tctgggctcg acaggtgtgt caggtcaccg gtgtggatga cgaaatccgg
     1201 tgtgtagccg aggttgttga tctgatcgat tgcgtggtcg aacgtcttgg tgacgtccgg
     1261 gttggcccca ccggtgaatc cgatgtggct gtcgctgact tgggcgaatc gcagcgtcgg
     1321 ccggccctga gcatgctctg ccgaagccga gccggcgacg tgcgagatga cttcgccgcc
     1381 ggcaacggcc agaccgaccg ccgccccgaa ccatgccgtg tgccggatca gctggcggcg
     1441 gttcatggtg tgcgggtctt gggtcatgag cgccgctcct tcccatcgct tcgttctgca
     1501 tcgtcgccgg cgcggctcat tgcgtcacca ccacggtgcc gtgcatggac gggtgaatcg
     1561 agcagacgta gtcgaacttt cccgcgctgg gaaaggtgtg cgaatatgtg gcccccgttc
     1621 ccatcccggg cgagtgaaac gacccgtcgg ccgcggccac ggtgtgtggt tcttcgtcgc
     1681 ggttggtcca ggtaacggtg ctgccggcgc gaacggtcag cgtcgccggg gcgaaggcga
     1741 agttgtcgat ggtgaccgcg tcacccgcga ccgccacggg cgaatctgtt gccgacgacg
     1801 tgccggccgg tgcactcggc atcccgggca tgccgctcat gcccgacatt ccgggcgtcc
     1861 cgccggcgga gccgaaggta atcgatggct gcgcatcgtg ccgcgacgcc gagcatcccg
     1921 ccagcaacag cgcacccacg gccagtacgg ccaagtgttt tcgaggcatg gtgatcatgc
     1981 caaaagatag gaaggtgggt tacaccagtc aggccggctc gtcctcgcgc ggcggggcga
     2041 acacctcgat tgacccgttg gcctcgcgaa ccatcaacgt aggcaatggc tttggggcga
     2101 tcggcagttg atgggtaagc acctggccgc tgggcgaaaa cgacgtcgta tggcaggggc
     2161 aacgcagcgt gtcgtcgggc gcgtcgaacc acagccggca gccttgatgc gtgcacacgc
     2221 cggacacggc ctcaactcgg ccgtcgacgc ggcgcacgaa tccagtgacc gaacccagat
     2281 cgaacggatg catcgtggcg tcgggcacat ccgcgctgcc cgcgacacgc tgccagcttc
     2341 cgtcgttggg cgtgagctgc cccgctacgg catcgccgcc gggggcttcg ccgggaccgc
     2401 cgatcacggc tcgatcgatg gcaaccgctg ccaccgcggc ggccgcggcc gccgacgtgc
     2461 ccacgatgac ttggcggcgc gtcgcgcttt gcctgggtcc tgccttgggc gttgtgccgt
     2521 gcatttgctc ggcgagacga cggtgcaggt cggtgaggaa ttcctggcgc ggggcgtcat
     2581 caccctgccg agcagcccgc aactcgatcg ccgttcggat ctgcgccgcc tcgaagtcgt
     2641 cgggtgcaaa cggcttgggc cggcgacccc ccaacaggtc gtcaacgtac cgccgcaagc
     2701 ctcgcgcgtt catggctggc ctccctcgtt gacctgtgcc gccagccgca gagcgcggtg
     2761 ttgcagcact ttggcgttgg caacgctgat cccaagttcg gcagcggact ccttgatcga
     2821 attgccttgt aagaaacgca tttccaaaat ctggcgatac cggtccggca gccgatcgag
     2881 gacctgggcg acacgctggg gcgccgtgct gatcgcttcc tcgctctcgg gtggttgttc
     2941 gatgtcgtcg atcgaggtta tctctcgtcc caaggtctcg cgccagtgcg cggccagcac
     3001 cgttctggcc gtggcgcgca agtatgcgcg cacctcgccg acactggcac tcaggcgcag
     3061 cggccgcaac gcggcgagga acacctcagc ggtgaggtcc tcggcgtcgg cccggttgcc
     3121 gacccgggcg aacagcgtgc gatacaccca cgccgcgttg tcctggtaca cggcttccca
     3181 gtcggggtag ccactgaagc cagcgtcata gccactgtcg ggcaccgcgc gcagcggtcg
     3241 cgggccgatg ggttcgttgt gtgccgccac gtccctcctt caccgaccaa tatcagctcg
     3301 ggttacacac cgggtcacgg cgcggtgagt gctggttcct cgaccatcaa ccggtcgagt
     3361 ttgccggcgg tggccgacag cgctgatttg gccatgctca gtatccggtc gcgcacccgg
     3421 ggattcgccg accccggcgc ctgtaggtgg cccagcagat gccccaagac gtcgaggcgc
     3481 gccgcggcga gccaggccgt caagtccggg tcgtcgagcc agcgcagttg attgccatag
     3541 tcggccagcg tgatgcgcaa ccagtccagg tcggtgtcgg ggtgcggtag cggcacgcac
     3601 agccggttct tgacggagtc gtccgggtca ttgagatgcg tcgcctcgac gtgggcgacc
     3661 aacgcggcgc tgaatacctg ttggcacccc cgcacgcttt gtagcgtgat ccggccgtca
     3721 tcgaaaatgg gtgtagcggg ccgcttttcg gcgccgtcgg cggtgcagtc gaggaacagc
     3781 gcgcggccgt ggatcggcac ggtacccccg tcgaggatca tttggtcggc gtcggtacgg
     3841 agcaggtggc ccatgcgcac cacatcggtg atggcccgca gttgctccag ttcggcctga
     3901 gtgacggtcg cgcagcggta catggagggc cggatcgccg gatcgattcg cagcagggcg
     3961 ccggcatcct ccaggcgaca gaacaggtcc tcgaccgacg ccgcgtccgc gatggcccgc
     4021 acccgcgcgg cgaagttggc cttgatcagg tcggcgaaca acggtcccgg ctggaccatc
     4081 gcgcgatcca gcagccagga gtcgcggggc atgatccagg tcagccgatc cggggcgatg
     4141 ccctggccca gcagccatag gcacgtgtcg atgccggtct tgccggcccc caccacgacg
     4201 tagcgctcgt gggcggggcg gcgcggcagg tcgttcggcg cgacgcattc cacgcccgcg
     4261 gcaacccggt agggcggcgg gcgcatcgag ggaaccatga cgcgcaggta cgtggcgtcg
     4321 acgacgcggc gtgccacgtt gacgacgtaa tcggtcccgg ccagcgtcgc aaagcggcca
     4381 tcgccgcggt attcgctcat cgggaagtag gagacccgcc cggtcggcag caagtgttgg
     4441 cgcatcaccc ggtcgtagta ggcgcagatt tccccggcgg tcgctagttc gtacagccca
     4501 acgttgcagc cgacttggtc gatggtgtcg ctaccgaggc gcagcgagtt cactccgtag
     4561 aacgccgagg gctgatgcag ccgcacgaac gggtaggcca tggtccaatg cccgcccggc
     4621 tggtggttgc gatcgaccag caccacgcgt gcctcggttt ccgagaccag cgtgtcgacg
     4681 aacgccatgc ccatggcgcc cgcgccgacc accaggtagt cggcctcgat ggtgcgcata
     4741 tcggctaaag gctaacccga cttgccagca ccaggagaag ttactaggat atcctagtat
     4801 ctaatctgct tttgcgcatt cgaggacacc catgaccaca cagatcccgc acttcatcga
     4861 cgggaagcgc accgccggcc agtccaaccg caccgccgac gtgttcaacc ccagcaccgg
     4921 ccgggtgcag gcgaaagttc cgatggccgg ccaggccgat gtcgacgctg cggtggcctc
     4981 ggccgccgaa gcccaaaagg gctgggccgc atggaacccc cagcgccgcg cccgggtgat
     5041 gatgcgattc atcgagctgg tcgacgaggc aggcgacgag ttggccgagc tgctgtccct
     5101 cgaacacggc aagacgctgg ccgatgcccg cggcgacatc cagcgcggca tcgaggtgat
     5161 cgagttctgc gtcgggatcc cgcacctgct caaaggcgag tacagcgaag gcgccggtgc
     5221 gggcatcgac gtctactcgc tgcgccagcc gctcggggtg gtcgcgggca tcaccccgtt
     5281 caatttcccg gcgatgatcc cgctgtggaa ggccggaccg gcgctggcgt gcggaaatgc
     5341 gttcttgctc aagccaagtg agcgtgaccc gtcggttccg gtgcggctcg ccgagctgtt
     5401 catcgaggca ggcctgccac ccggcgtgtt ccaggtcgtg cacggcgaca aggaggccgt
     5461 cgacgcgatc ctgcatcacc ccgacatcaa ggcggtgggt ttcgtcggca gctccgatat
     5521 cgcgcagtac atctactcga cgtccgcggc gaccggcaag cggtcgcaat gcttcggcgg
     5581 agccaagaac cacatgatcg tgatgcccga cgcggacctc gagcaggccg tcgacgcgct
     5641 gatcggcgcc ggatacggca gcgctggcga gcgttgcatg gccatcagcg tcgcggtgcc
     5701 ggtcggcgag cagaccgcgg aacggttgcg cgccaggctg gttgagcgga tcaaccactt
     5761 gcgggtgggc cacagcctgg accccaaggc cgactacggt ccgctggtca ccgaggccgc
     5821 gttgacgcgg gtgcgcgatt acatcgacca gggcgtggcc gccggcgccg agatcgtggt
     5881 cgacggccgc gaccggcgca gcgacgacct cgctttcggt gacgcgaacc tggagggcgg
     5941 cttcttcatc gggccgaccc tgttcgatca cgtcaccccc gacatgtcga tctacaccga
     6001 tgagattttc gggccggtgc tgtgcatggt ccgcgcgcac gactacgaag aagcgctgcg
     6061 gctgccctcg gagcacgagt acggcaacgg cgtggcggtg ttcacccgcg acggcgacac
     6121 cgcccgcgat ttcgtgtccc gggtgcaggt gggcatggtc ggcgtcaacg tgccgattcc
     6181 ggttccggtg gcctatcaca ccttcggcgg ctggaagcgt tccggcttcg gcgacctcaa
     6241 ccagcacggc ccggcgtcga ttcagttcta caccaaggtc aaaaccgtca cgtcgcggtg
     6301 gccgtcgggc atcaaggatg gcgccgaatt cgtcatcccg acgatgaatt aagtctgcat
     6361 taggaccgct tcatggatct attcatgctg aacgacgacg aacgcgtcat caccgagacg
     6421 gccgccgcgt tcgccgcgaa acgggtcgcc ccgcatgccc tggaatggga tgccacccag
     6481 cactttccgg tggacgtgtt gcgcgaggcc gccgaactcg gcatggcggc gatctactgc
     6541 cgcgatgacg tcggcggtag cgggctgcgc cgactcgacg gggtccgcat tttcgagcag
     6601 ttggccatcg ccgaccccgt caccgcggcg ttcctgtcca tccacaacat gtgcgcgtgg
     6661 atgatcgaca ccttcggcac cgacgaacag cgcaaggaat gggtcccgag attggcgccg
     6721 atggacgcca tcgcgagcta ttgcctgacc gagccgggcg ccgggtccga cgccagcgcc
     6781 ctgaccacgc gcgccgtcaa gcagggtggc gactacgtgc tcgacggcgt gaagcagttc
     6841 atctccggcg cgggcgcctc ggacgtctac gtggtaatgg ccaggaccgg cggccaggaa
     6901 aagccggggc cgcgcggcat atcggcgttc atcatcgaaa aggacactcc gggactgagt
     6961 ttcggcgcgc atgaacagaa aatgggctgg cacgcgcaac ccaccgcaca ggtgatcctg
     7021 gacggggtgc gggtgcccgc cgacgcgatg ttgggcggcg ccgagggcga gggtgccggc
     7081 ttcggcatcg cgatgaacgg cctcaacggt ggccggctca acatcgcggc gtgctcgctc
     7141 ggtggtgcgc tggccgcctt cgacaaggcg ggagcctacg tgcgggagcg acacgcattc
     7201 ggctcgtccc tgctcgacga gccgaccatt cggttcgccc tggccgacat ggccaccggt
     7261 ctggagacgt cgcgaatgat gttgtggcgg gccgcgaatg cgttggacac cgatgacccc
     7321 gacaaggtcg agctgtgcgc gatggccaag cgctacgtga ccgacacgtg cttcgaggtg
     7381 gcggacaagg ccctgcaact acacggcggc tacggctacc tgcgcgagta tggtctggaa
     7441 aagatcgttc gcgatctgcg ggtgcatcgc atcctggaag ggacgaacga aatcatgcgg
     7501 gtcgtcatcg gccgagccga ggccgcgcgg ttccgcgcaa ccctttagaa aggcagcaat
     7561 gacagagctt ttccaggtcg ctttcctagg tttgggcaat atgggcgggc ccatgtcggc
     7621 gaacctggtc accgccggcc acaccgtgcg cgggttcgac cccgtgcccg cggcggtggc
     7681 cgccgcggcc gcgggcgggg tcgcggtgtt cgacagcgcg gccgacgccg tgtccgaggc
     7741 cgacgtcgtc atcaccatgc tgcccaacgg cgacgtggtc aaacgctgct acgccgagat
     7801 catgcccggc gcgcgggccg gtgcattgtt catcgacagt tccacgatct cggtcaacga
     7861 cgcccgcgag gtacacgcac tggcggaatc acacggcctc tcccagctcg acgcacccgt
     7921 gtccggcggg gtgaagggcg ccgtcgccgg gaccctggcg ttcatggtgg gcggcgacga
     7981 gtccgcgctg cagcgcgcgc ggccggtgct ggaaccgatg gcgggcaaga tcattcactg
     8041 cggtgcggcg ggggcaggtc aggccgccaa ggtgtgcaac aacatggtgc tggcggtgca
     8101 acagatcgtg atcggtgagg cgttcgtgct cgccgagaat ctgggactct cggcgcagtc
     8161 ccttttcgac gtgatcaccg gcgcgaccgg caattgctgg gcggtgcaca ccaactgccc
     8221 ggtgccgggc ccggtgccga catcaccggc caacaacgac ttcaaaccgg gcttcgcgac
     8281 cgcgctgatg aacaaggacc tgggcctggc gatggatgcg gtggcgtcaa cctgttcggc
     8341 ggcgccgctg ggcacgcacg ccgccgagat ctacgccaag ttcgcggccg accacggcgc
     8401 cctggacttc agcgccgtga tcgaggcgct gcgctcgagt tgatgcggcc cgaatcaaag
     8461 accttgtgtc acggcacctt tcgccccagg aaggcggcca gttgatcggc cggtgcgcgc
     8521 tcggatgagc acggctgctc ctcggcgaag agcttgcccg tgccacggaa ctgggcgccc
     8581 acgaaggcac gcgccgcggc caactgctcg gccgccaact cggggtcgag atcggtggac
     8641 tggccggtgg ccgcggcgag atcccatgcg tgggtcagca cgtcggtggt gcgaattccg
     8701 acgaagagtt gcgcggggaa ttcgccgaac ggcagcttga agatgccgtc catgccgccc
     8761 ggtgccgcga agatctcctg tgccgcggcg gcgctggccc ggtgtgccgc agcggtgtcg
     8821 tcgggccggg cggtcggctg ctggttgctg gccgaccaaa tcccgacgta ctcgttgcct
     8881 ccgatgacat gctcgatcag atcgcgaatc gtccactccg aacagggcgt cgggccgtcg
     8941 aactggtcgg ggctgacatt cgcgaggacg ccggcgaatg tgtcctgcgc acgctggtga
     9001 gcaactagtg gatccactgt cctgcctttc cagtcctttt caagccttgt cacgttcgag
     9061 gttagaagtc gccggcgttg cgccgcaacg attcgatgga cgacaccagc gcttcggatt
     9121 cgccggcgtc cattccgacg tcggcgaaca cctgctggtt gagcgtaacc gtggcgtcgt
     9181 cgaccgtggc acggccgagg tcggtgatct ggaccaacgt cgtgcgcccg tcggtggggt
     9241 gcggtaagcg ctgaaccagc ccatcagcct ccagcctgcg aatggcgtgg gtgacgctgg
     9301 tgacgtggac ctgcaggcga tcggacgcct tggtgatcgg cagcgctccc gtgcggctga
     9361 acgccagcag ccgcagcaac tcaaagcggg agaagctcaa gtcgtaggga cgcaacgcgg
     9421 tttcgacacg ggccagcagg atttgatgcg cgcgcatcac cgaggtgacc gcgaccatgc
     9481 cctgcgacac ctcggcccaa ccggcgcgct cccagttggc ccgcgcggcg gcgatcggat
     9541 cgcgcttctg aggtcgtgct gcggccacgc cctttcttac cgcatctagg accctggcgt
     9601 ggcctttccc atcgcggcca gcaccgccag cgtcgagccg cggttgccga cggtaatccg
     9661 cgcgccaccg tcggcgtaac agcgaacgtg cagtccgccg tcggcgaata cctcatgcca
     9721 cgatctaccg cgcccacccc tcgaaggcag gtagatgaag ttcgcatggg catcggtggt
     9781 gtagatcccc agtgcgctca ggcgcatccg gagatagcgg cgctcggcgt tgatcgactg
     9841 gatccgttgc agcagttggt cttcggctcg gtacgaggcc gccaccgcaa gcatactggt
     9901 gatcgcgatg ccgaacggca gctgctggga ccacagctcg cgggccagtt ccggcgatgc
     9961 cagcccgtaa ccgatccgca ggccggccag cccgtatgcc ttggagaatg tccgcaccac
    10021 cacgacgttg gggaaacggg tgaccaacgc cgcaacgtcg acgcggtgct caggcgcgac
    10081 gaactcgacg tacgcctcgt cgagcagcac gatggtgtcc cgcggcaccc gtcgcaagaa
    10141 ccgcaccagc tcggcggcgg gctccagcgt gccggtcggg ttgtgtgggc ggcataccac
    10201 caccacgcgg gcgttcgccg cggcgtcggc cagcccgtcg aggtcgttgt gaccgtcctc
    10261 gtcgaggggc acggtcaccg cgttcagccg gcccatccgc gcaacgatcg aataaccgtc
    10321 gaaggtcggc gacgccatgg cgatcgtgtc acccggcgtg gtcagtgcca gcagggcctg
    10381 caacaccaca ccggtggcgc cggcgcccag caccacctgg tccgatggca ggtcgatgtg
    10441 gtgggcgatg agactgcgca gccgttcggg caggaactcc ggataccggt tggccgcaca
    10501 gatggagcgg atcaacgccg accgcaccgc cggcagcggc gggaacggat tctcgttgag
    10561 cgacaacgca agcggatcga ccgcagtggg aagcgcgtcg tcgatctcgg gcgcgaaagg
    10621 gcttgggacg ggttgcatca gcctcgcccg ccccagcgca ccgccgccgc accggcgaaa
    10681 tcgccggcgt gggcgaaggc cgccatcatg accacgtcgc ccgccttgac ctgaccgtcg
    10741 gatatcgcgc ggtcgaggtt gaccgggatc ccggccccga acaggttgcc gcactcttca
    10801 aaggtgtccc gatggcgttc gggcggcaat tcgagcgctt cccgccaatt gcgcaggaac
    10861 gcccggttgg gctgattggt gaccagtagg tcgatgtctt tggcgggcag gccgattcgg
    10921 tcgcagaccg ccagcgccac ctcgggaacc tgccggttgc cgcgggccag caccttggtg
    10981 atcttgcttt cggtgaagcc gatgctgccc tcgcccggcc cggcagccca ccatttgcgg
    11041 ggcgggtcgt aggagagcgt catttcgccg gcgtatgccc cgtaggtgcg gcattccaca
    11101 tcgagaatcg gcgactcgtc gctcgccgtg accaggccca ccgccgcccc gtcgccgggc
    11161 accgccgact gcgccttgcg gcgaatcgtg ggctggtcga agaattgccc ggccgcattc
    11221 tgcgcgatcg cgatcagcgc ggtgtgcccc tctccggtcg acagcagttt gcgagccacg
    11281 ttgagcgcca acacaaacgc cgcgcagcca ccgttgttca ggtcgagcac ccaggacggc
    11341 cgcaagccca ggcggtgcgc gatgccgccg ccttcaccgt agaacgccat gtccggcacc
    11401 tgggtgtggg tgatcaacac gtcggcattt tcgacggcgt cgtggccgtg gcgttcgatc
    11461 aagccctgcg ccgcgcgctc gaccatgtcg atcgcgcttt cgtccgatcc gacgtgatgc
    11521 cgaaacttgg gcgcgcggaa catgaggttg tcgcggaggt cgtcggattc ggcgaattgc
    11581 gcgtagtact cggccgggat cggttcaccg ggtagataag tggagacgtc gatcaggctg
    11641 acgctcggct tgctcatgct gtttctcact cgttctcacc gcatccacac gggggtcacc
    11701 ggcaggccgt tgcggtggcg gtactcggcg attgccttga ggttcttcat ttccaacagg
    11761 tggccggcgc cgaacatgtc ccagaagtcg ccgacccaca ccgggcgctg cggcggtgcg
    11821 gtctccggat acgggttgcg gtcgtagaac gggtgacggc aattggtcca cagcacaacc
    11881 gaacccggct tgtcgagcac cacctgggcg tcgatgacgc gcatcagata gatcatccac
    11941 aggtgcttgc cctgatccca cgcgcagtgg tagtcgacgg tgcgggcgtg gctgttggcg
    12001 atggtccggg tgtagatctt ggtctccgag ccgagccggt cgtaggccag ccacaggccg
    12061 ggctcgtcgg tggtggtgaa accccgcaga ctgtaggtcc actcctcgag gcttcgagtg
    12121 tcggccaggt attcgtacaa ctcgtccggc gggcagtcga tgtagtcgtt gacggtgcaa
    12181 tactccccga aaacctgatc gtgtgggtac accgaccgca tcatgtccat gatgatcggg
    12241 gtggccttct cccgggggct ggtctcgata cggaccaccc cctcgatggg cgtacggata
    12301 tcctcaagcg ctggcagcga catcggtccg gttctccttt gcaatggaac tgtcttgtgt
    12361 gagatccgct ttggccggct gcgtgttgag aaatggtgca aaaggcggga tctcgtcggc
    12421 ggcacactcg acactgacca cggcagggcc gtcgacggcc agcaccccgc gcatggcagc
    12481 ggcgaagccc tcgaggtcgg tgacgtcgac ggacgtcagc ccggggaaca tcgccgccag
    12541 gccggcaccc aagcggctgg agcgaaatcg gttgtagctg tagagatctt cgtagaagag
    12601 ccgctcacgg gtcacgcaca tggcgtgggc attgttgttg aacaacacga acgtcaccgg
    12661 gagctggtaa tgcacggcgg tatggacctc catgccgtgc atgaaaaacg ccccgtcccc
    12721 ggcgatcacg acggtgcggc cgcccggtcg tccgctgttc gggcgcccga aagccatccc
    12781 gatgccggcg ccgaagctgt agcccatgcc acccatgccg agcgcgaccg cgaacctgcc
    12841 gccgcgccgt gccggcaggt agtggatcgc cgccgcgccg gtgttgccgg cgtcgaccac
    12901 gatgtccgcc ccgtcgggca cgcaaccgtc cagcaccgcc atcgcgtcgc ggtagcgcac
    12961 cccggcgccg aggcagggcg ggggtgtcag ttcggtgcgc cgcaccacat ccggcacccg
    13021 cacaccagtc ggccgtcccg ctccggacag cgcctgggtc agcatgcgca gcgagccgcg
    13081 caggtcgtcg gtgtgcacgt gggtgcacga aacgtagggc ggcgccgatc cgatcgagac
    13141 cgtccgcgcc gccgcaaggg cgtcgtcgag tccggtgcgc gcggtgaccg acagtcgggt
    13201 gcccaccacc aggcacacgg cgctgtcggc caccgcctgg gccacacccg ggtggcccat
    13261 gacaccggtc accccgagcg ccgacgacga cccgagcccc ggcgtgcccg cgacgtcctt
    13321 ggcgtcgggc acggtcgcca cccgtgcccg caacaccgca cgcagcgcct cgagttcggc
    13381 gcgggcgtta tcccgggcca cctgttcgcc gacgatgagg gtcaccgggc cgcgcgcgtg
    13441 gcgcagtatt ccgacgatcg gttgggggtt accgatggga cgcagaccct gggtggccaa
    13501 cccacttcga gttccggcac gctcccggtc cacgcgcccc tgctgaatgt ccttgggcag
    13561 caacaacact gcggggccgc cggtgcgcgc tgcggcgatc gccgccgaca gcgccggtac
    13621 gacgccggcc ggggtgagca cccgctcgca gaagagggac accgcggaaa acaccgcctg
    13681 cgcgttcaac gagccgttgt cgccactggt gtcctgaaag ctgccccgcc cgtccatggt
    13741 ggtggccgtt tggccgacca gtgccaacac cggaacccgg ctcgtcagcg actcccccag
    13801 ggccgctacg agattcagtg cgccgccacc cgaggtcgcc gcgaccaccc ccagcccggc
    13861 cccgctgcgg ctgtacccgt cggccatggt ggcggcggag aactcgtgct tggccaggac
    13921 cgcggtgatg tcggagcgga agtgtgcggc gtcgtacagg tcctcgatgt tggcgccgtc
    13981 gacgccgaag atgtggtcga ccccgattgc cgcgaggcgt tcgacgatgt ggtcgaccac
    14041 ccggtgcgtc cttggcatgt ctttaacacg acacgcgcgt accttcagtt cactccggcg
    14101 ccgggtgtta catcgagcgc tgcagcagaa cctcgccgct gtcagcggaa accacttgca
    14161 cggcggcaat ctggtcgacc ggcgtggaga tgctgcccgc gggcgtcgcg gtgtggccgg
    14221 gttcggccac ccacgttgcc agccgggttt ggctgccgtc gcgacccacc accaccatcg
    14281 ccagggtgtc atggtgggca ttcagcggcg ccaggcagac acacttgagg ttgatggacg
    14341 tcccccagtg ctggctggac agctgcaccg tcgaggccag caggttcgta ccgacctgca
    14401 ccatcggctg cgaggatgcg gtcacctgct gcgcgggtgc cgaggtgtag ccctgcacac
    14461 cgacgaacac cccgattccg agcaccgcgg ccgcagcggc cgaggccacc caggtcgcca
    14521 cccgcgtgcg gcgccggcgc cagcgcaccg ttgccagcaa cgacggcagc aactccggcg
    14581 acaatgcagg cgtctccggc aaagactcag cacggccgtt gatcgcggcc acttctgctc
    14641 ggtcaagccg cgaaagcagg gccggcacgc cactcatatc ggcgacggcc tcccggcatg
    14701 ccgggcagtt ggccatgtgc tcctcgaatt cacggcggtc ggaggcagac agcgacccca
    14761 acacgtatgc ggcatcccac atcgcgtagc ggtggttgtc acgcccagtc aggtcgaaca
    14821 cctcgttgcc gtgattgtca tagggagggc cgataccccg taacggcgtc ctcatttcat
    14881 ccatctcctg tcaccctcca agtattcgga gccgaaagcc agtcggatgg gttcggaaat
    14941 aaacccctgc gctgtcatcg ggtaacccca agttcctgca gagtgagtcg caatgcccgc
    15001 acggcatagt gtagtcgcga cttcactgtt ccttcggcga tctcaagatc tgcagcaatc
    15061 tgcgcggtgg tccagccgcg gtagtaggac cgttcgatca cggcccggtg ctctggcgac
    15121 aattggacca acgcatcggc gatcagcagc cggtccagcg ccgcgttcac ctcgtcgggc
    15181 gtggactgtt cgggcgcgcc cgcttcgtcg agggagccga cgacgttgcg gtaccgtgcg
    15241 ctgcgtcggt cgtcgatgat catgttgcgg gcgacggtga acagccacgc ccgcgctgac
    15301 cgttcggtgt ccccgattac ctcgggatgc tgccacgccc gtaacagcgt ctcctgcacg
    15361 acgtcctcgg cctggctcgc gtcccccgtc aaccggagcg cgtagcgcca taaaaccgcg
    15421 gcatgctcgt catagagcgc cttcatcagg gcggcttcgg ctgccccgga cgccccccga
    15481 gtactggcca cacgagccac tcgatcacct ccgcctactg acacgagtgg agtaggtgtt
    15541 tagttcaagc cagggtccag ctcaagcgag gcgtggttca accgagagtg aggatgcgcg
    15601 ggcccccgtc ggttaccgcg acggtgtgtt cccagtgtgc cgcacgtgac ccatcggccg
    15661 tagtcacggt ccattggtcg tcgagcacca ccgtcttgct ggttcccagc gtcagcatcg
    15721 gttcgatggc caacaccgag cccggggcca gcagcgggcc ccgcccggga gcgccctcgt
    15781 tgggcaggaa gggatccatg tgcatgtgcc ggccgatgcc gtggccgccg tagccctcca
    15841 cgatcccgaa cgaacgtccg tagcgagcct cggcggcgcg ggtccccatt tcgatggcat
    15901 gtgccacgtc ggacagccgg ttaccggcga ccatcgccgc gattccggct tccagcgatt
    15961 ccctggtcgc atcgccgagc gcttcgtcgg cggcgtccag cgccccgacc ccgaaggtga
    16021 tcgcggcatc accgtgccag ccgtccagca ccgcgccgca gtcgatggat accaggtcac
    16081 ccggtgccaa tatctccgcg gcggacggga tgccgtgcac aacgcgatcg ttgaccgatg
    16141 cgcaaatcga tgccgggtaa ccgtgatagc ccaggaatga cggtgtcgcg ccggcctcgc
    16201 ggatcaccga ctcggcgatt tcgtcgaggc ccagcgtcga cgttccggac accgcggcag
    16261 cgtggaccgt gtgcagcgcg gcggcgacca cggccccggc cgcggccatc gcgtcgagtt
    16321 cacccgggct gcgctgcggc acgaccctgc gactgcgcag ccgcgccaac gggctcatgg
    16381 ttacttgccc agcgcttcca gggcgcgggc gaacacctca tccatcgagc ccaccgcgtc
    16441 gacggtcttc agctggtctt tcaattgatc cccgtagtac tccaacagcg gcgcggtctc
    16501 gtcgcggtaa accttcatcc ggttgaggat gacgtcgttg gtgtcatcgg cgcgtccgcg
    16561 tgccttaagc cgcgcaaaca actcgtcctg cgagacgcgg aactccacaa ccgcgtcgat
    16621 gtcggtgccc cggcgtccga gcatctcgtg cagcgctttg gcctgctcga gcgagcgcgg
    16681 atagccgtcc aggatgaagc cgtcggccgt atccggatcg ttgagacggt cgtcgacgag
    16741 ctggttggtc aaatcggagg gcaccaggtc accggcggcc aggtaacgct tggcctccag
    16801 cccgagcgtg gtgccgttgg cgatgttgcg ccgaaacagc tcgccggtgg agatttgcgg
    16861 gatttcgagc ttctcggcga gcttcacggc ctgcgtgccc ttgcccgccc ccggcggtcc
    16921 cagcaaaacg actctcactt gaggaaccct tcgtagttgc gctgcatgag ctgactttcg
    16981 atttgcttga ccgtatccaa accgacgcca atcatgatca aaaccgcagt accgccgaac
    17041 ggcaagtttt ggacgcctcc gccattgccg atctgaagga aaagattggg cagcacggct
    17101 atcaccccga ggtagatcga gccgggcagg gtaatccggt tcagcacata acgcaggtag
    17161 tccgcggtcg gctttccggg gcgaatgccc ggaatgaagc caccgaactt cttcatctcg
    17221 tcggcgcgtt cgtcgggatt gaaggtgacc gacacgtaga agtacgtgaa gaagatgatc
    17281 agtccgaagt aaatggcgat gtagaccggg tcgctcgggt ccgacaggta ggtgccgacg
    17341 aatttgtccc accaactgtt gccgacaccc ccggtgccgc tacggaccag ctgggtgatc
    17401 aggtgcggaa tgtagatcag cgacgacgca aagatgaccg gaataacccc ggcctggttg
    17461 accttgagcg gcagatacgt cgaggttccg ccgtacatgc gccgtccgac catccgcttg
    17521 gcgtattgca ccgggatccg gcgctggccc tgctcgacga acaccacgcc gacgatgatg
    17581 atcagcgcgg cgacgcagac ggcggcgaag atgatcccgc cgcggctgtc caggatggac
    17641 ttgccttcgg cggggatgcg tgccgcgatg ccgacgaaaa tcagcaacga catgccgttg
    17701 ccgatgccgc gttcggtgat cagctcaccc atccacatca ccagcgcggc gccggccgtc
    17761 atcaccagca cgatgacgac cagggtgaag atgctctggt cggcgatgat gtccagcgag
    17821 cagccctgga gcagaccgcc gttggccgcc agcgccacga tgctggtggc ttgcaggatg
    17881 gccagcgcga tcgccaggta acgggtgtac tgggtcatct tggcctgacc cgattggccc
    17941 tccttgcgca actcctcgaa gcgcgggatg accaccgtga gcagctgcac gatgatgctc
    18001 gcggtgatgt agggcatcac cccgaccgcg aacaccgtga gcttcagcag cgcgccaccg
    18061 gagaacaggt tgatcagcga gtagatctgt ccggcctcgc cgccgctggc ctccttgatg
    18121 cactgctgca cgttcgggta gttcacgccg ggggacggta atgcggcgcc gacccgatag
    18181 agaaggacga tgccaagtgt gaagaggatc ttccgtctca ggtcgactgt ccgcagcgat
    18241 gagatgaaag ccgaaagcac tcttctcctc ctgcgctacc gtgggtcatc ctgcatcacg
    18301 cgcccaacac ccgcaggcca ggacgtacgg cgtcgcgcgt caaaccgtgt acgagactaa
    18361 cagctgatcg ggcgagagcc tttcaaggcc ctttcaaggg cctttcaggg gccgttcagg
    18421 ggccgttcaa gtccggtcga ggggcaggtc gctgcggccg cgtaggaacc cccacaaggc
    18481 acaggcgacg ctcagcgacg aggcgtacag tgctagctgg ctcattgctt ttgctaagta
    18541 tcgaaggccg tccggggagc aatctatggc gcacctgagc agcacacggt ttaacggcgg
    18601 cacgtgtggt cagacggtgg ggctgcggca acgccacggc atcgatctcg acagggcccc
    18661 ctgacccgcc ggccaggcgt ccttcgccga gcccgccgct gcgccgggac cagccgaacc
    18721 aacaactaaa agccgctcaa accgcaggag caccatgaca cgcaccgatc aagacagctg
    18781 ggacctggcc tcgagtgtcg gcgccacggc gaccatggtc gccgcagccc gcgcgttggc
    18841 cagctcagag accgacgcga tcatcagcga cccgttcgcc gcgccattgg tgcgggcggt
    18901 cggcgtcgac ttcttcatcc ggctggttga cggtgccgtc ctcccggcgg acgcgccgga
    18961 gggacagcgg gacctgcggc tggaaacgga ttcgatcgcg gtccgcaccc gctttttcga
    19021 cgacttcttc ctcaacgccg cccgcgacgg catcaaacaa tcggtgatcc tggccgccgg
    19081 cctcgacgcg cgcgcctacc ggctggcctg gccgccgggg agcgcggtct acgaagtcga
    19141 tcagcccaag gtcatcgagt tcaaggacgc ggccatggcg aacctgagcg cggccccgtc
    19201 cgcggatcgg agaaccgtca gcatcgatct gcgggaggat tggccggctg cgctgcgccg
    19261 cagcggattt gacgtcacgc aaccaacttc ctggagcgcc gagggcttgc tgatgtacct
    19321 cccgccggac gctcaggatc gccttttcga caagatcacc gaactcagcg ctcccggcag
    19381 caaactggcc accgaatacc acggcgattc cggcgagacg atgaccgccc gcgcacagca
    19441 attcaacgat cgatgggcca atctcggctg cgacatcaac ctgtcgggat tgttcttcga
    19501 cggcgagcgc agcaatgtcg tcgactacct gaccgagcgg ggctggcaag tcaccaccag
    19561 gccccggcgt gactacttcg ccgattacgg ccgcgtcttc ccggaggacg agacatccca
    19621 gcttcgcaac atcgtcgccc ttaccgctgt ccgcatctag gggcactccc atgaccgaca
    19681 ccaagcgatt tgagggcgac agttgggatc tggcgtccag cgtgggggcc accgcgacgg
    19741 ccgtcgccgc gcggcgggcg atggcatcga agggaccgaa tccactgatc gacgatccgt
    19801 tcgccgagcc gctggtgaac gccgtcggtg tcgacgcctt catccggatg atgaacggcg
    19861 aaatcgaggt tgccgaagac gatcccgcct tcacggcgca acggctcagc gaaggaatgg
    19921 cggtccgcac ccggttcttc gactcgttct tcctcagcgc cgcggaagcc ggcgtccgcc
    19981 aggctgtcat cttggcctcc ggactcgaca cccgcgccta ccggctgccg tggccggccg
    20041 ggaccgttgt ctacgaaatc gatcagccgc aggtgatcga gttcaagacc cgcaccctgg
    20101 ccgatcttgg cgcgaccccg acggcggacc gcagggcggt agggatcgac ctgcgcgatg
    20161 actggccggc cgcgttacgc gccagcggat taggcccgac gcggccgacg gcctggatcg
    20221 ccgaaggact gctcgggtac ctgccgccgg acgcccagga tcgcctgttc gacaacatca
    20281 ccgcactgtc cgctcccggt agccgcatcg gtaccggcta tgttcccgac attggtgacc
    20341 gcgtccaaaa gcgcggtcgc gagctcagcg aacgctggcg acgtatgggg ttgaatctca
    20401 actggtccga attggtttac gccgacgagc gcaacgatgt ggtggcctac ctttccgacc
    20461 gtggctggca gacgacggtg agtagcacac cggagatgta cgcgcgcaac ggttttgagt
    20521 tcccgaccga accgtccttg gtcgcgttcg gcgatatcaa gtacgtatcc gcaaccttga
    20581 cgtagggggt cgcaagcagt atggccatgg cgcgcaccga ccaagacacc tgggatctgg
    20641 catcgagcgt gggagcgacc gcgacctggg tcgccgccgc ccgggcgttg gccagcaggc
    20701 ggcccgacgc actgatcgac gatccgttcg ccgacccact ggttcgcgcg gtgggcaagg
    20761 aattcttcgt gcgggttctc gacggcgaga tcaccgatga aagcggcggc ctcgacccgg
    20821 aggccgatcc cgacgcggag ttcaacctgc agcggatggt caacatgatg gccgtgcgca
    20881 cgcggtactt cgacgacttc ttcaccgacg cggccgccgc cggcgtccgc caggccgtga
    20941 ttctggcggc cggcctggac tcgcgtccgt accggctggc gtggccggcg ggcaccgtca
    21001 cttacgaagt cgatcaaccc gccgtcatcg cgttcaagtc cacgacgttg tcgaacctcg
    21061 gcgccgagcc caccgcggag cgccggaccg tttctgtcga ccttcgcgat gattggctga
    21121 ccgcgttgcg ggacaagggt ttcgacgatt cgataccgag cgcgtggagc gccgaaggcc
    21181 tgctgatgta cctgccaccc gaggcgcagg acaggctgtt cgacgccatc accacgctca
    21241 gcgctccggg cagttggctc gccaccgaat accatcgcgg aggtatgccg ttgctcgaga
    21301 ggcgcgccaa ggcgatggcg cgccgctggc agccatacgg cttcgacgtc gacgtctcaa
    21361 ctctggtcta tttcggcgag cgcaccccgg cggcagagta tttgagcgct cgcggctggc
    21421 aggtctcgac gtgtacccgc gccgacatgt tcgccgcggc cggactgccg ataccgaggg
    21481 gtgacagcct ggaatcgctg cagaacagca tcgccgtgac cgccgttctg cggtaacgct
    21541 cagcgcgggt cgaccgccgg cgaacggacg accatcggca ccgcggggaa acaccacgcc
    21601 atctcggagc gcgacctgcg cagtgcggcg gtgccgtagc ccttctttcg gtagtcgggg
    21661 tgaatccaga tccgcacatc cacctcgccg tggaccaact cgccgaagac catcccgacc
    21721 ttctgccccg aatcgacggc caccaaccag gtggcctctt catcgtcgac ccgcgccagc
    21781 gccgcgcgga tctcgtcgtc gaggttgccg gccggtccgc ccgagccgtc gccggccgcg
    21841 cccatgtcct cggtgcgagc ggcgaagatg tcgcggtcct cctcggcgga aaagggccgg
    21901 agctcgaggg tgtcccccga gctggccggg cgctctttga gcgtgaaact cagctgcttg
    21961 ttcaggtcct cgagttcgga ctgaattttc ctgcgcgaat ccttggtgag ctgatcgaag
    22021 gacatgccca tgaccgcttc ggcgccgaca tgggaggttt cgagcaaagc cccgatcgct
    22081 tcgacggcgg ccgccttgtc ctcggactcc accatgaggt cgagtacttc atgccgacgt
    22141 tcgagagctt tcagcagggc gtctgcgatc tctcgacggg cagctttgcg atcctggtca
    22201 gtcattgcac aagcctagaa cgccaccggg ggtatcgcga gatcctcggt ttcccagttc
    22261 caggaaccgt tggtagcggt cctcgatcgc tgccgcccag gcggggtcgg ggtcgacggt
    22321 gcactcgatg gagacccagc gggcggcatc ggcgatcgag gtttccaggc cggccgcgat
    22381 gcggcccagg aaggccgctc caagtgccgc cccctcggct accccggaca cctggaccgg
    22441 ccgcccggtg gcgtcggcga tggcctgcag ccacggccgg attctgatgc cgccacccgc
    22501 agccacgacg cgcgacaccg ccgctccgct cagctcgatg agctgacgga cgacgaagcc
    22561 cgacgcctcg taggcggccc gccgcagcga ggcggcgtca tgcgtgagat cgaggccggc
    22621 gagcatcgcg cggcgatccg gatcgtgcaa cggcgtgcgc tccccgcgga tatacggcga
    22681 ccacaccggt acccggcggg gatcgacggc cgtcgggtcg cccggtccca caacgcgatc
    22741 cacccatccg agaaacagcc cgcccgcgtt gctggctccc ccgatctggc tcttaccggg
    22801 agcaacgtgc ggaatcgtcc acagcccggg cacctgccgg gcctgcggaa tggtcgtcca
    22861 cacgatcaag gtggtgccgc acagcaccag cacgtcgccg tcgtggtcgg cgccggccac
    22921 catctgttcg cacagggcgt cgatagtccc gatgcccagc accgccggag tcccgtgaac
    22981 ccgtccggcc gccgctccga tcgtctccgc gcgtggcatc tgttcgacgg tcgcgccgcg
    23041 ctcggcgcag gcggccgggt tccagccggt tccgtcgaag aggggaaacg ccgtggcggc
    23101 cgtggcgaag tcgatcaccg cctcgccggc cagggcatgg ttggccaccg ccggcgccgg
    23161 ccagtacccc gccgcgtcgg gcgcctggcc gaccgtccag cgcaggaact cggtcgcttc
    23221 acccagcgcc ggaagcagcg gggccggttg tccggggaca ctcggagccc ggcccctgcc
    23281 gtcgccgtac agcagacccg gggtgagcgg ccggcctgcc gagtcgacgg cggtcagcga
    23341 aggcgccatc gccgagaccg ccaccgcgtg ggcgttcgga cacccgagct gatccagggc
    23401 cgccaacgga ccccgccgcc acgcccgccc ggcgtcgtgc tcgagccgat cgggggccgg
    23461 gacccgcagc tcgtgcggaa tgcgcacccg cgccgtcacg cggccgtcgg cgtcggcggc
    23521 gaccgctttg accgcggtgc ggccgatgtc gatgccgatt gtgacgtctt cacgtgacac
    23581 tggcgtcacc gtacgccagc atgggggttc gtgacctcaa gaccgcgcgc cctcgtgaca
    23641 gccccgctgc gtggaccggg gttcgccaag ctgcagcagc tggccgacgt ggtctacgac
    23701 ccttggatag gtgagctcgg cgcgacgctg cggatctaca acggcgagca gctggccgag
    23761 cggatcgctg ccgaatccgc tgatgtcgtt gtggtggaaa gtgattcggt acgcggcccg
    23821 gtgttcgacc tgggcttgcg cgcgatcgcg gccacccggg gagaccccaa caacgtcgat
    23881 gtcgagggag ccaccgcggc cggtatcccg gtgctgaaca ccccggcccg caacgccgac
    23941 gcggtcgccg agctggcggt ggccctgctg ctcgccacca cccgccacgt ggtgacggcc
    24001 gacgccgatg tacgcagcgg caacatgttt cgcgatggca gcattcccta tcagcgattc
    24061 cgcggctggg agatcgcagg acgcaccgcc ggactggtgg gcctcggcgc cgtcggccgc
    24121 gcactgcggt ggcggctgtc cggcctgggc atgcgggtca tcgcccacga cccgtataac
    24181 gacgaagccc ggcacggcct ggacgaactc ctgggtgagt ctgacgtggt gtcgctgcat
    24241 gcgccggtca ccgacgacac cgttggcatg atcggtgcgg ggcagttcgc ggccatgcgt
    24301 gacggtgcgg tcttcctcaa caccgcccgg gcccagctac acgacaccga cgcgctcgtc
    24361 cacgcgctgc gcggcggcaa gctggccgcc gccggcctgg atcacttcgc cggcgaatgg
    24421 ctgcccatcg accacccgct ggtgggcatg cccaacgtcg tcctgacccc gcatatcggc
    24481 ggcgccacgt ggaacactga ggctcggcag gcgcagatgg tggccgacga cctggaagcg
    24541 ctgctgtccg gcaagacacc cgctcatatc gtcaacccgg aggtgctggc ctcatgaagt
    24601 tcgtggatag tccagaaacc gcggtgctgg acgcggccaa ggacatgctg cgccgcggtc
    24661 tggttgaggg caccgccggc aacatctcgg cgcgccgctc cgacggcaac atcgtcatca
    24721 cgccgtcgtc ggtcgactat cgcgatatgc agctcgatga cctcgtgctg gtcgacccgg
    24781 acggcgccgt cctgcaggcc gcggcgggtc gctcgccgtc gtcggaatcc aagctgcatc
    24841 tggcgtgcta ccacgcgttc gacgacatcg gcagcgtcat tcacagccac ccggtgtggg
    24901 cgaccatgtt cgcgatcgcg caccagccga tccccgcctg catcgacgag ttcgcggtct
    24961 attgcggcgg ggacgtgcgg tgcaccgagt acgccgcgtc cggcacaccc gaggttggcg
    25021 ccaacgcggt caaggcgctc gacggccggg cggcggccct gatcgccaac catggcctgg
    25081 tggccgtggg gcctcgaccg gacaaggtcc tgcacgtcac cgccctggtc gagcgcaccg
    25141 cccaaatcgt ctggggtgca agagctctcg gtggaccggt gcctattccc gaggacgtca
    25201 accgcaactt cgcaatggtc tacagctatc tgcgcgccaa ccctctgtaa cgacgccgag
    25261 tgtgaaccac gcgacgggac acgccgtcaa gccgtcgtga gattcactct gggcgttagg
    25321 tattgggcgg cttcgccccg tctagcgctg gtcgatggca accttcacgg ccccgctccg
    25381 gccctggttg gcgatcgcga ggaaggcatc gcgccactgc tcgagcccga aggtgtgggt
    25441 cagcatagga cgcaggtcca cccggcccgc ggccaccagg tcgaggtagt gggcgatggc
    25501 gtgcttgcgc tggccttcga cctcttcgat cccgaaggca ttgctgccca cccagctgat
    25561 ctccttgaag tacagcggac tccactccca tcgtcccggc gcgtggacgc ctgctttcac
    25621 caaggtccca cgggcgcaca agacccgcac ccccacttcg aacgtctccg gcttgccgat
    25681 cgtgtcgtac accacctcga ccgctccggg gtaggccatc ggcaagccca gcagcggccg
    25741 gcgcagccgg ccaccgcccc aggcgacgac gtcctcgatg acggccaggc tcggttcgtg
    25801 cgccagcacc aatgacgccc cgaaccggcg ggccagttcc gcctgggccc cgaagcgggc
    25861 caccaccgcg accgccacgt cggggtagag cgcccgcagg atcgcgaccg cgcacaaccc
    25921 gagcgacccg gccccataca ccaggactcg gcccgcgcgg ggcggggggt ggcgggtgat
    25981 cgcgtgcagc gacaccgaga acgggtcggc gaacacggcc aattcgtcgg gaatcgactc
    26041 cggaacggcg aacagcatgc tgtcgtgcgc cggcatcaat tcggcgtatc cgcccgtcac
    26101 atctgccgag accccggtgt ggatgccggg cttgatgtcg ccgtcggtga agctccagca
    26161 caggctgtag tcgccgttct cgcaggcggg gcagggcggc tcgacgccgc gcggcccgca
    26221 ggacagccac gggttgagca ccacgcgctg cccctcctgc agccctcgtg cctccggacc
    26281 cacctcgacc acgtcggcga ccacctcgtg acccatcacc tgggggaagg aacagaaggc
    26341 ggccatggcg ttatcggcgt cgccctcgcc gaaatccatc aggatctgct tggagtccga
    26401 cccgcagatg ccggtcagcc gcggccgggt gatcacccaa tcccgatgca gcagccgcgg
    26461 atccggcacc tctcgcagcg ccgtcggggt tcgggccaag ttcttggtca gcgtgttggc
    26521 gccatcggga atcgcgtacg gctccggcgg cacaccatag acgagcgcct tcatcgcggc
    26581 gccctaaggc cggcgttcgc ggcgtcgacg acctcttgag tgtccgggac gtagtgctcg
    26641 aattcgtcgc cgcccaaggc cgcccccaaa cccggcgaag cgccggtgct gagcacccgt
    26701 ttgccggccg ccgtgtacgc catcaatcaa ggttgccgcg attcggttcg ggcgtacagt
    26761 gtggagcttg ttatactgac taagtattag atgaagtaat tgcgtccgat aaggggaaat
    26821 gcatgacgtc cactcgctac gagggagata cctgggacct ggcgtccagc gtcggcgtga
    26881 ccgcgacgat ggtggccgcg gcccgcgcga tggccagcag ggtcgaccgt ccgctgatcg
    26941 acgatccgta cgcggagccg ctggtcaggg cggtcggtgt ggacctgctg acccggctgg
    27001 ccagcggcga gttggatccc gacgatttga acgatgtaca tgacggcgcg accggatccg
    27061 ccggcgccat gtcccgcatg gccgacaaca tggcggtgcg gaccaagttc ttcgacgagt
    27121 tcttcctggg cgccacgaac gccggtatca agcaggccgt gatcctggcc tccggcctgg
    27181 acgcccgcgc gtaccgtttg ccgtggcccg ccggaaccgt ggtctacgag gtcgaccagc
    27241 cgcaagtcat cgaattcaaa tcccgcacgc tggccgagct gggcgccgca cccaccgccg
    27301 aacggcgtgc tgtggctatc gacctgcgcg aggactggcc cgccgcattg cgcgccgccg
    27361 gattcgatcc ggcccagccg accgcatgga gcgccgaagg cctgcttggc tacctgccac
    27421 ccgaggccca ggaccgcctg ctggacacca ttaccgaact cagcgcgccg gggagccggc
    27481 tcgccaccga aagtgcaccc aacccggagc ccggtgacga ggagaagatg agggagcgca
    27541 tgcaggcgat ctccgagcgt tggcgtgccc acggcttcga tctcgacatg gccggtctcg
    27601 tctacttcgg cgagcgcaac gaagcagccc cctacctgag cgggcacggc tggcagctga
    27661 ccagtgccag cattcgagaa ctgttcgcag acaacggcct tccgccgttc gaggacgacg
    27721 acatgcgcat gggcgaaatg ctctacacca gcggcaccct gaactcgaac gcgaaatagg
    27781 agctaccgga atggctatcg actcgggacg cactgacggt gacagctggg gcccggcatc
    27841 cagcgtggga gcgacggcca ccatggtcgc ggcctcccgc gccgtggcgt cgcaggggcc
    27901 caacgcgctg ctcgacgatc cgctggccga tccgctggtc cgcgcggtgg ggttggatcc
    27961 cttcgtccgc atcatcgacg gagagatcga tttcgaggat gacccgctgt tcaaccgcaa
    28021 agcacggact gagcagatca cggtgcggac caggttcttc gatgacttct tcatcggcgc
    28081 tacccaagac ggcgtgcggc aggcggtgat tctggcgtcc ggcctggaca cccgggcgta
    28141 ccgcctgcgc tggccggagg gcacggtggt ctacgagatc gaccagccac aggtcatcgc
    28201 cttcaagacc aacacgctgg ccggtatcgg cgccaatccg accgccgacc gccgtgcgat
    28261 cgccatcgac ctacgagacg actggcctgc ggcgttgcgg cagggcggtt ttgatgtcac
    28321 gcaaccgacc gcgtggagtg ccgaaggcct gttgccctac ctgccgcccg aagctcagga
    28381 ccgattgttc gacaacatca ccgcgctcag cgcgccgggc agccggctgg ccaccgaaca
    28441 cgtgccagac ccgaacgcgt tcaccgatga gcggttgcag cgcatcagcg agcgctggca
    28501 gcgcttcggt ttcaacctca acgccgcgga cctcttctac cccggcgagc gcaatgtcgt
    28561 cgtcgactac ctgggcaccc acggctgggc ggtgacggcc catcctgcca gggaactgta
    28621 tgcccagaac ggctttgaat ttcccgagga cgagatggcg gccaccttcg gcgaactcag
    28681 ctatgtcgcc gcgatcctga agcagggggt tggctagggt cgtcccatgg cacgcgctca
    28741 cgacgataac tgggatctgg cgtccagcgt gggcgcgacc gcgacgatgg tcgcggccgg
    28801 gcgcgccatg gcgaccaaag atccccgcgg tttgatcgac gacccgttcg ccgaaccact
    28861 ggtgcgcgcg gtcgggatcg atttcttcac caggatgatc gacggtgagc tggacctcga
    28921 tgcgatcgag aacctgacac cggtgcgcat gcaggcaatc gtcgacggga tggcggtgcg
    28981 cacgaagtac ttcgacgatt acttcgccga cgccaccggc agcggggtgc agcaggtggt
    29041 gatcctggca tccggcttgg actcgcgcgc gtaccggttg gcgtggccac ccggcacggt
    29101 cgtctacgag atcgaccaac cacaggtcat cgagttcaag accaccacgt tggacggcat
    29161 cggtgcccaa cccaccgccg tccggcgcac cgtggccatc gatctgcgtg cggactggcc
    29221 ggccgcactg cgagcggcgg gattggacac gacggcgccg acggcatggc tggccgaagg
    29281 tctgctgatc tacttgccgc cggaggccca agaccggttg ttcgacaaca tcaccgcgct
    29341 cagcgtgcca ggcagcacga tcgccaccga attcgggccc ggcatcatcg atttcgacgc
    29401 cgaccgggtg cggcagatgt ccggcgcctt ccgtgaccag ggcatcgaca tcgacatggc
    29461 gtcgctggtc tatgccggcg agcgcaacca cgtggtggac tatctgggcg ccaaaggatg
    29521 ggatgcgcag ggagtgacgc gaacggaact cttcaaacgg caccacatcg acgtgcccgc
    29581 ccccgaacag gacgacccgc tcggcgagat catcttcatc agcggccgac tcgcctagcg
    29641 gtcggttaga ggcgcgagtt ccccagccag agcacgctgg cgccgctcag cgtctgctcg
    29701 acgttgtcca ggatcccggc gacggagcgg gccagtagcg cccccaacgc atccggcagc
    29761 gacgccgcag ccggccgcga cgaggttcgg ggccgcacga agtccagcag cgaagacccc
    29821 ggatagttga tgatgcgaac ttcggtgtcc gcgtccaggc cggccaggac tttggcccgc
    29881 cggaccgcgg ttcgaaaccc gccgagttcg tcgacgaggc ctcgcgccag cgcatccgcg
    29941 ccggtccaaa ctcgcccccg ggcaacgact tccacggcct cagtggtcag cttgcgacct
    30001 tcggcggccc gtgtcacaaa gtcggtgtag aacaagtcgg cctcggcctc gcgatgggcc
    30061 cgctgctgcg gcgtgaaggg cgcatcgacc gaccaggcgt cggcattggc gttggtgcgc
    30121 acggtatccg acccgacgcc cagccggtcc ttcaggtcgc ggaccaccag ctttccggtg
    30181 atcaccccga tggagccggt gatcgttccc ggatcggcca cgatcgcgtc ggcgcccatc
    30241 gacacgtagt agccgccgga cgcggcaacc gaacccatcg acgccaccac cggcttgccg
    30301 cgttcgcgag cccttttcac ctcgcgccag atggtctccg acgcggtgac cgagcccccg
    30361 gggctgtcca cccgcagcac gatcgcggac accgaatcgt cggccgcggc ctcccgcagc
    30421 gccgccgcga tggtgtcgcc gccgacgttg gaggtgccca gcggcaggaa ttgaggccca
    30481 ccacgcccgt tgacgatcgc gccatcgatg gtgaccacag ccaacgtcgg cttggtccgg
    30541 cgaccgggga ccgggggcat cggcggggcc agtcgtgctc gggccgcacc ggcgtagcgt
    30601 gccagataga gccgcggtgg cgactcttcg tccgaaacat cttccacacc aactagttcc
    30661 gcgatgcgcg cataagcttc gtcgcggaat ccgatccggt cgaccaggcc ggaggcaacc
    30721 gcgtcgtcgc gcagcagcgg ggcccggtcg gccaattcat cgagcgctgc cggatcgatc
    30781 ttgcgtgatt gcgcgacagc ctgccacacc tgttcttgca ggctttccag catgcgggtg
    30841 acggcctcgc ggtgcgcatc ggtaaagccg tcctcggtga aaaggtttgc cgccgacttg
    30901 tattcgcccc gcgcgacaaa ctgggcttcg atccccgcct tggcgagcgc gtcgcgcagg
    30961 aacgtggcgt tgctcgcaaa gccgatcagc cccacacttc ccgagggctg catccagacc
    31021 tcgccgaacg ccgaagcgag gtaataggac agcgtgcccg gataggtctc ggcccaggcc
    31081 agtgacggtt tgacggcgct gaaggccgcg atggcctccc gcagctcctg caccaccgcg
    31141 ggcggcgacg ccgcgagctg cacacgggca atcagcccgg cgacccgggg gtcctcggcg
    31201 gcacggtgga tcgcggcgac gacgtcgcgc agcgccatcg gccggctgcc cccggtgatg
    31261 atcgccagcg ggtccaagcc cgtcgtctcc ggtggcgctg agcgcaggct gaattcgagc
    31321 acgcagccgt tgggcacgcc gtggtggcgg gcggtatcga cccggccaac cagggcacgg
    31381 acgtcgtcaa ccctgtctag caggccgggg atggagggca ggaaggcaaa catattcgcc
    31441 cagcgtacct accgtacggt ggacggggtg aggttctcga tcgctatccc ccaactcgac
    31501 gcagatacct tcgacggccc cggccttcgc tcctaccttg tccgcgccga ggagctcggg
    31561 ttcgagggcg gctgggtgct cgagcagatc atcggggccg caccgttatt ggctccgctg
    31621 gaactgctgg cgtactgcgc ggcctgcacc gagcgactgc gcctgggggt cgcggtgctg
    31681 gtgacctcgc tgcacgagcc acttcaggtg gcggcgtctg tgacggccgt cgaccggctc
    31741 agccacggac ggctggacgt cggtgtcgcc ccaggtgggg gtgctcgcaa cttcgccgcg
    31801 ttcggcgtcg acaaggacac cttcatctcg tatttcaccg agggcctgga actgatgaag
    31861 gcggcctggt ccgatgaacc cagggtcacg ttccacggcc gcttccgcga cgtcgacgat
    31921 ctgcccgtcc agccgaagcc ggtgcagcgg ccacatccgc cgatctggtt cggcggcctc
    31981 gctcccaagg cactggccag agcggtccgc ctcggtgatt cgttcttggg cgccggctcg
    32041 tcgaccaccg aggcgtttgc cggagcggtc gcggtggtgc gacgtgaact cgacgaacag
    32101 cagaaagacc ccacgcactt caccatcggc aagcgcgtct atttgatggt cgacgacgac
    32161 gcgccacggg cccgcgagcg cgtgctcacc gggctgcacc gcatctacgg cgaaatgcgc
    32221 ggcatcgacg cggttcccgt cttcggcacg cccgacgacg tcgcccgcgg gctgcgtgag
    32281 gtgatcgacg ccggcgccga gatgctgcta ctcaatccgg tcggcgccga tgtcccggaa
    32341 aaccgcgaac agatggaact ccttgcggca gaggtgattc cgcagctggt ttagctgtac
    32401 accttgatcg gccgagcaga cgcagaatcg cacgaatccg cgacgcggcg tgcgattctg
    32461 cgtctgctcg cgaggggcag gtgcccttta cagctcggtg gccgaaccgc ccgcggcggt
    32521 gatcttctcg cgggcgctgc cgctgaactt gtgcgcagac acgtcgacct tgacggtcaa
    32581 ctcgccgtcc ccgagaacct tgaccaagga gttcttgcga accgcaccct tggccaccag
    32641 gtcgcccacg ccgatcacgc cgccctgagg gaagagccgc tcgatgtcgc cgacgttgac
    32701 gatctcgtat tcggtgcgga accgattccg gaatcccttg agcttgggca gccgcatatg
    32761 aatcggcatc tgcccgccct cgaaggtcac cggcacgcct ttacgggcct tggtgccctt
    32821 ggtgccacgg ccggcggtct tgcccttaga gccctcaccg cgaccgacgc gggtgcgtgc
    32881 ggtcttcgac ccgggggcgg gcttcaggtc gtgcaacttg atcgtcattt ggtgttacct
    32941 ccggcggccg cctcggctgg ctccaccaca acaaggtggt tgaccaccgc gatcaggccg
    33001 cgcgtctgtg ggttgtcctc tcggatcacc gaatggcgga tccgacgcaa acccagggtg
    33061 cgcaggctct cgcgctgctt ccagcgggca ccgatcgtcc cgcgtacctg ggtgatcttc
    33121 agctgactca tggctgcggg gttccctccc gagtggcgct cgcactggcc agcgcttcac
    33181 tttcccggcg cgccttgagc attccggccg gggcgacgtc ctcgatcggc agccctcggc
    33241 gggcggccac ctcctcggga cgctgaagga gcttgagcgc ggcgacggtg gcgtggacca
    33301 cgttgatcgc gttgtcgctg ccgagtgatt tggccaggat gtcgtgcacg ccggcgcatt
    33361 ccagcaccgc gcgagccgca cctccggcga tcacgccggt acccggactg gccgggcgca
    33421 gcagcaccac gccggcggcc gcctcaccct ggaccgggtg cgtgatggtg ccgccgatca
    33481 acgggacgcg gaagaagcct ttgcgggcct cttcgacacc cttggcgatg gcggcgggca
    33541 cctccttggc cttgccgtag ccgacaccga ccatgccgtt gccgtcaccc acgatgacca
    33601 aagcggtgaa gctgaagcgc cgaccaccct tgaccacctt ggagacgcgg ttgatcgcga
    33661 cgacccgttc caggtagttg ctcttttcgc cgtcgcggtc gcggccacgg ccaccgccgc
    33721 cgccgtcacg ccggccccgg ctgtcgcggt caccgcggcc gtcaccgcgg gtgtcttggc
    33781 cctgcccgcc ggccgactgt tccgccatta cgcggtcctt ccagttgtca tcagaaattc
    33841 aatccgttct cgcgcgcggc atcggccagc gccgcgatcc gtccgccgta ggtgtatccg
    33901 ccacggtcga acaccgcgct gtcaacaccg gcggccttgg cacgctcggc gatcagttga
    33961 cccacccgca cactgcgggc tttcttgtcg ccctgcaggc cgcgcacatc ggtctcgatc
    34021 gacgacgccg cggcgaccgt ggtgccgttc tcgtcgttga ccagttgcac gtgaatgtgc
    34081 cgggcggacc tgtgcaccac cagccggggg cgctgcgagg tgcccgcgat cttcttgcgc
    34141 agtcgtgagt gcctgcgcag ccgtgacacc cgccgagttg cggatgcgct ctgccccacc
    34201 ggctttcgcg cggtggtgtc agcctttgat tgcgccatgt ctacttacct gtctttccga
    34261 ccttgcggcg gatctgctca ccctcgtagc gcacgccctt gcccttgtac gggtcgggac
    34321 ggcgcagacg gcgaatattc gccgagatct ggccgacctg ctgcttgtcg atcccagaga
    34381 tcgtgaactt cgtcggtgac tggaccgcga acgtgatgcc gtcgggcgcc tcgatcacca
    34441 cggggtggct gtaacccagc gcgaactcca ggttggaacc cttgagctgc acgcggtaac
    34501 cgaccccgaa gatctccatc ttggtggtgt aaccctgcgt cacaccggtg accagattgg
    34561 acaccagggt gcgcgacagc ccgtgcagcg agcggttgtg ccgctcgtcg tcgggacggg
    34621 tgaccacgat cgcaccgtcg tcgctgcgcg ccaccctgat cggctcggcg accgtcagtt
    34681 ccagggtgcc cttgggcccc ttcaccgaga cgttctggcc ctcgatcgtt acgtcgactc
    34741 cggcgggaac cggaaccggc tgcttaccaa tgcgcgacat cgtttccccc tctcaccaca
    34801 cgtatgcgag tacttcgccg ccgacgccct ggcgggctgc ctgacggtcg gtgagcaggc
    34861 ccgaggacgt cgagatgatc gccacgccca ggccgccgag cacccgcggc agattggtgg
    34921 atttcgcgta cacccgcaac cccggcttgg acacccggcg cagtccggcg atgctgcgct
    34981 cccggctcgg cccgtacttg agctggacga tcagcgcctt gccgacccga gcgtcttcgg
    35041 tccggtagtc ggtgatgtag ccctcggcct tgaggatctg ggcgatgttg gccttgatct
    35101 tcgagtgggg cagcgtcacc tcgtcgtgat acgccgagtt ggcgttgcgc agacgtgtca
    35161 agaagtctgc gatcgggtcc gtcatagtca tcgagcgcta ctcctcttca tcgcggcggc
    35221 cccctcgccg ctgcgcgtcc gggggcgccc ccactgcatc gtcgataacg cggtcatgac
    35281 agcgtctgtc accttcctcg cggtggttcc ctgctggggc ctgtcgcgcg cctgttctgg
    35341 gttgggtcta tgtcctgtta ggagaggctg ttaccagctg ctcttctgca caccgggcag
    35401 ctcacccgcg tgcgccatct cgcgcaggca gatcctgcac aggccgaact tgcggtagac
    35461 cgcacgcggg cgtccgcact tgctgcaacg cgtgtaggcg cgcaccgcga agcgcggttt
    35521 gcgattcgcc ttgttgatca gtgccttctt agccattgct cacgatcccc tttgaggttc
    35581 ttccttgaac ggaaatccga gggcccgcaa cagcgctcgc ccttcgtcgt cggtcgtcgc
    35641 cgaagtgacg acgttgatgt ccatgccgcg gacccggtcg atcttgtcca cgtcgatctc
    35701 gtggaacacc gactgctcgg ccagtccgaa ggtgtagttg ccgacgccgt cgaactgctt
    35761 gggcgaaaga ccgcggaagt cacgaatacg cggcagcgcg atcgacgtga gccgatcgag
    35821 gaactcccac atccggtctc cgcgcaaggt cacccggacg ccgatcggca tgccctcgcg
    35881 cagtttgaac tgcgcgatgg acttgcgcgc cttacggatt tccggccgct gcccggtgat
    35941 cagcgccagg tcgttgaccg cgccgttgat cagcttggcg tcgcgagccg cctcgccgac
    36001 tcccatgttg acgacaacct tggtcacgcc cgggatctgc atgacgttgc cgtagccgaa
    36061 ctgcttgtgc aaggcatcgc gaatctcgtt gcggtagcgc tctttcaggc gcggctgcac
    36121 cttcacgggc ctttcagagc tttctgtagt ggtcataccc tcagatgtcc ttgccgttgc
    36181 gcttggagat acggacgcgc ttgccggtct cctcgtcgac gcggtaaccg actcgggtgg
    36241 gcttaccgtc cgagtcgaca accatcacgt tggaggcgtg gatcggggct tcctgggtga
    36301 cgatcccacc cgactgcgca ccgcgctgcg tggtcgaaac cggggtgtgc ttcttgatcg
    36361 cgttgacacc ctggaccagc acccggttgc gctccgggta gaccttgagc accttgcctt
    36421 tggcgccctt gtccttacct gcgatcacca ggacggtgtc gtcctttttg atcttcatct
    36481 acaacacctc cggagccagc gagatgatct tcatgaatcg cttctcgcgc agttcgcggc
    36541 cgacgggccc gaagatgcgc gtcccgcgcg ggtcgttgtc gggcttgatg atcaccgcgg
    36601 cgttctcgtc gaacttgatg tagctgccgt cggggcgcct gcgctccttg acagtccgca
    36661 ccacgacggc cttgacgacg tcaccacgtt tgacgttgcc gccgggaatg gcctccttga
    36721 cggtggcgac gatgatgtca ccgattccgg cgtagcgtcg cgacgaacca ccgagaaccc
    36781 ggatgcacaa gatctccttg gcaccggtgt tgtcggcgac cttcagccgc gattcctgct
    36841 gaatcactcg agctcctgac ctggttcacg tgtgcacggc aggacaaaac caacgtagtg
    36901 agtacctgac gtgcgcggtc tttgtcgccc aagagcacgc ggggccgatt cgcaccggcc
    36961 gaggtctgcc caaggcaacc cctagagtct aggggacgac cagctcagag ccaaatttga
    37021 caccaaagcg gcatcagaga tcaatgacca gcccgtcgtc agcccagccc cagaacgttt
    37081 tcagacggtc acccgtgcac gcccgcgccg ccaggtcacg gaacgctatc ccacccctgg
    37141 cccggatcgc cgttgcacca acgagtggag acccaagtcc cagggccagg cccgccgagg
    37201 acggcgaggc agcctccagt atcagtcgtt acctggccgt tgggctggat actccaatgt
    37261 tgggcgtacg tatcgataca gggcccgagg tgcgaaaacc aagcgtcccc cgggattgtc
    37321 aggcaattcc cggggaaggc cacgctctcc agctgccggc cgttgagatt ccagcgctga
    37381 aaatccgaac cattgcaggg gttgatcacc accgcggcgt accagccgcc actcggggcg
    37441 tccagacaaa catcacctaa tcggcttctc aactgggccg ggccgtcggc ggccgccgca
    37501 ccggcgctta tcaccgcgac accgagcact gtgctggcca cgacaacgcc ccggcagaca
    37561 ccgcccatta acggcgacac atgcatcgtc agctccatct cctggtcctc gtcaaccaca
    37621 gccagtacac gcgatgcagc cataccgata ccaccacggc caccgacgac ttgccgcaag
    37681 aaatgtattt ctttacccca gtaccgtccc gcggccaccg cttcggcggc agggaacacg
    37741 aaagggaacg ccttagttgg agtgtcgata ccaggcggcg atatacagca cgagagcagt
    37801 ggccagcgcg atcagcaccc agatcacgac agcttggtcg atccacgaac ccgcg
//
LOCUS       DASSGQ010000259         3466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_625522,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000259 DASSGQ010000000
VERSION     DASSGQ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625522"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="VFI55_11520"
     CDS             complement(<1..1373)
                     /locus_tag="VFI55_11520"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HET7074696.1"
                     /translation="MGATKVVDREALSAALDALDATFDEVAEFDCETLTTREQLALLE
                     RFERLRRRLPAIEHPLINSIARQATAEELGGKLSHAIAEWTLTSRAEASRRIKEAADL
                     GPRHGPTGEPLAPVLAATAAAQRKGELGAGQVAVIRRFYHQLPGWVDAATREQVETRL
                     AREGTQYRPEQLAGLADTLADCLNPDGAYTDEDRARRRGVTLGNQEADGMSALRGWLT
                     PEARATVEALLAKLAAPGVCNPADNAPTVDGPPSQDAIDKDTRSAGQRNHDALLAALH
                     ALLASGNLGQHNGLPAAIIVTTTLTELETAAGKGLTGGGTILPMSDVIRLARHAHQYR
                     AVFANGQALALYHTKWLASPTQRIVLYAKDRGCSAPGCEVKGYYCEVHHTTDYAKCHT
                     TDVNQLTFACGTHHRILQPGGWTTRANTRGDTQWIPPPHLDRGQPRTNTFHHPEKLLR
                     DDGNADG"
     gene            1796..2947
                     /locus_tag="VFI55_11525"
     CDS             1796..2947
                     /locus_tag="VFI55_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HET7074697.1"
                     /translation="MRFLHTADWQLGMTRHFLAGDAQPRYSAARRDAVAGLGLLAAEV
                     GAEFVVVAGDVFEHNQLAPQVIGQSLEAMRAIGIPVYLLPGNHDPLDASSVYTSALFT
                     AERPDNVVVLDRAGVHRVRPGLEIVAAPWRSKAPTTDLVAQVLQGLPEGPVTRVLVAH
                     GGVDVLDPDREKPSLIRLAALDDALRSGVVHYVALGDKHSLTEVGASGRVWYSGSPEV
                     TNFDDVEADPGHVLVVDIDESDPRRAVTVESRRVGRWRFATMHRQVDTSRDIVDLDLN
                     LDLLTEKDRTVVRLALTGSLTVTDRAALDACLDRYARLFAWLGLWERHTELAVIPADG
                     EFTDLGIGGFAAAAVDELVSTARAGDAESAVDAQGALALLLRLADRGAA"
     gene            2944..>3466
                     /locus_tag="VFI55_11530"
     CDS             2944..>3466
                     /locus_tag="VFI55_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7074698.1"
                     /translation="MKLHRLVLTNYRGIAHREIEFPDHGVVVVCGANEIGKSSMIEAL
                     DLLLESRDRSTKKEVKQVKPTNSDVGSEVCAEISCGAYRFVYRKRFHKRCETELMVLT
                     PRREQLTGDEAHERVRAMLAETVDNDLWHAQRVLQATSTSAVDLSGCDALSRALDVAA
                     AHSDSEAALSGTEP"
BASE COUNT          549 a         1083 c         1212 g          622 t
ORIGIN      
        1 tccccgtcgg cattcccgtc gtcgcggagg agtttttccg ggtggtgaaa cgtgttggtg
       61 cggggttggc cgcggtctaa gtgtggtggc gggatccatt gggtgtcgcc ccgggtgttt
      121 gcgcgggtgg tccagccgcc gggttgcagg atacggtggt gggtgccgca ggcgaaggtg
      181 agttggttga cgtcggtggt gtggcatttg gcgtagtcgg tggtgtggtg gacttcgcag
      241 tagtagccct tgacctcgca accgggcgcc gagcagccac gatccttggc gtagagcacg
      301 attcgttgtg tgggggaggc cagccatttg gtgtgataca gcgccaaggc ttggccgttg
      361 gcgaagaccg cgcggtactg gtgggcgtgg cgggccaggc ggatcacgtc actcatcggc
      421 agaatggtgc ctccaccggt gagccctttg ccggcggcgg tttccagctc ggtcagggtg
      481 gtggtgacga tgatggcggc gggcagccca ttgtgttggc ccaggtttcc cgaggccaac
      541 agggcgtgca gcgcggccag cagcgcgtcg tggttgcgct ggccggccga gcgggtgtct
      601 ttgtcgatcg cgtcttgtga gggtgggccg tccacggtcg gggcgttgtc ggcggggttg
      661 cacaccccgg gggcggccag tttggccagc agggcctcaa cggtggcgcg ggcttcgggg
      721 gtcagccagc cgcgcagcgc cgacatgcca tcggcttctt gattacccaa cgtcacaccg
      781 cggcgccggg cgcggtcttc gtcggtgtag gcgccgtcgg ggttgaggca gtcggccagc
      841 gtgtcggcca gcccggccaa ttgttcgggc cggtactggg tgccttcgcg ggccaaccgg
      901 gtttcgacct gctcacgggt ggcggcatcg acccagccgg ggagctgatg gtagaagcgg
      961 cggatcaccg ccacctgccc ggccccgagc tcgcctttcc gttgtgcggc ggcagtggcg
     1021 gccaacaccg gggcaagcgg ctcaccggtc ggcccgtggc gcggtcccag gtcggcggct
     1081 tctttgatac gccgggaggc ttcggcgcgg ctggtcagcg tccactcggc gatcgcgtgg
     1141 gataatttgc cgcccagctc ctcggcggtg gcctggcggg caatggaatt gatcagcggg
     1201 tgttcgatgg ccggcagccg ccgccgcagc cgttcgaagc gctccaacaa cgccagctgc
     1261 tcgcgagtgg tcaatgtctc gcagtcaaac tcggcgacct cgtcaaacgt ggcatccaag
     1321 gcatccaacg cggccgacaa cgcctcgcga tcaacaacct tggtggcacc cacaccgcta
     1381 aactatccga gcccaccgac aaccaacgcc gccctgagac tactgatacc gcagtgacac
     1441 aagggattta agccaacacg gtgcggtttg cgcgcagcgt gaaattaccg cacaactcgc
     1501 cagaaatcgt cgtttaggcc aatatccctg gctttcaaag caactaccgt cgcgcgtgga
     1561 cccgaacgca accgacctgg ggtcacgcgg ggcccacgat catgacctcg ccctgggagc
     1621 tcacgaacag atcgacgcac ccgtcgggca tcacgtccag cgaccgagcc ggcgagcctg
     1681 accgcaccca gccgcattcc acctattggc catgccggtg cgcatccccg gagttgtcgg
     1741 taggcggacc tagacttcgc gttgccttga ccaccacgta ctcaggagaa acgggatgcg
     1801 attcctgcac accgccgact ggcagctggg catgacgcgg cacttccttg ccggcgatgc
     1861 ccagccacgg tattcggcgg cccgtcgcga cgcggtggcc ggtctcggcc tgctggccgc
     1921 ggaggtgggc gccgagttcg ttgtggtggc gggtgacgtc ttcgaacaca atcagctcgc
     1981 cccgcaggtg atcggtcagt ccctggaggc catgcgtgcc atcggaattc cggtctatct
     2041 gctccccggc aaccacgatc cgctcgatgc gtcgtcggtg tacaccagcg cgctgttcac
     2101 cgccgaacgt cccgacaacg tagtggtgct ggatcgcgcc ggtgtccatc gggttcggcc
     2161 cggcctggaa atcgtcgctg cgccatggcg atccaaggcc ccgaccaccg acctggtcgc
     2221 ccaagttctc cagggcctcc cggagggccc ggtcacccgc gtgctcgtcg cccatggcgg
     2281 cgttgatgtg ctcgaccccg atcgtgagaa accgtcgctg atccggctcg ccgccctcga
     2341 cgacgccttg cgcagtggtg tggtgcacta tgtggcactg ggagacaagc attcgctcac
     2401 ggaggtgggg gccagcgggc gagtctggta ttcgggctcg cctgaggtca ccaacttcga
     2461 cgatgtcgaa gcggacccag gccatgttct ggtcgtggac atcgacgaaa gcgacccgcg
     2521 ccgcgccgtc accgtggagt cccggcgtgt cggccgctgg cggtttgcca cgatgcaccg
     2581 tcaggtcgac accagccgcg acatcgtcga ccttgacctg aatttggatc tgctgaccga
     2641 gaaggaccga acggtggtgc ggttggcgct gactggttcg ctgacggtca ccgaccgcgc
     2701 cgcattggat gcgtgcctgg acaggtacgc acggttattc gcttggctgg gcctgtggga
     2761 gcgccacacc gaactggcgg tcatacccgc tgacggcgag ttcaccgatc tcggcatcgg
     2821 ggggttcgcc gccgcggccg tcgacgaatt ggtgtcgacg gcacgtgcgg gcgatgcaga
     2881 gtcagccgtc gacgcgcagg gcgcgctcgc gttattgctg cgactcgcag atcggggcgc
     2941 cgcgtgaagc tgcacaggct ggtcctgacg aactaccgcg gcatcgcaca tcgcgaaatc
     3001 gagttccccg atcacggcgt ggtggtggtc tgtggagcca acgagatcgg caagtcgtcg
     3061 atgatcgagg cgttggacct gctgctggaa tccagggatc gctccacgaa gaaagaagtg
     3121 aagcaggtca agccgaccaa cagcgacgtc ggctctgagg tctgcgccga aatcagctgc
     3181 ggtgcttatc gtttcgtgta tcgtaagcgg tttcacaaga ggtgcgaaac cgaactgatg
     3241 gtgctgacgc cgcgccgtga acagctcacc ggcgacgaag cccacgaacg cgtccgggcc
     3301 atgctggccg agacggtgga caacgacctg tggcacgccc agcgggtgct gcaggccacg
     3361 tcgactagcg cggtggacct gtccgggtgt gatgcgctgt cgcgggctct ggatgtggcg
     3421 gctgcgcatt cggacagcga agcggcgctg tcggggaccg agccgt
//
LOCUS       DASSGQ010000260         3274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_200714,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000260 DASSGQ010000000
VERSION     DASSGQ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200714"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VFI55_11535"
     CDS             <1..846
                     /locus_tag="VFI55_11535"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET7074699.1"
                     /translation="IILVRVSGYGQTGPDAHKAGYASVAEAASGLRHLNGFPGGPPPR
                     LALSLGDSLAGMFGAQGALAALYRRSVTGHGQVVDVALTESCLAVQESTIPDYAVGGV
                     VRGPSGTRLDGIAPSNIYRSADGSWLVIAANQDTVFARLCKAMGRPELATDDRFATHG
                     ARGRNQDELDTIIGAWAAERQPGDIIETLSAAGVIAGPINTVAEVVNDPQLRARGMLV
                     EHYDERVGCDVLGPGVVPVLSESPGRVRHAGPARPGQHNEDVYVGLLGKTTEELGELR
                     AEGVL"
     gene            843..1739
                     /locus_tag="VFI55_11540"
     CDS             843..1739
                     /locus_tag="VFI55_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HET7074700.1"
                     /translation="MSPHVDIREVSLRDGLQIEKPIPLTAKLELLAAVAATGVREVEA
                     TAFVSPSKVPAMADAAELAAQLHDYPDIEFSALVAGPNGAKRAAAAGLRSIEYVVAAS
                     DAFSSANVGRNSAEATDQIEEIVAIAHDSNMSVEVIVATAWDCPFQGPTPPGRVLEIG
                     AAARDRGVDRFSIADTIGTTTPKRVSSLIAQLRPVIGDMPLGGHFHNTRGAGLASAYA
                     AVCSGVTRLDASVGGLGGCPFAPGATGNIATEDLVYLLRDSDIDVDIDLSAAIAAARV
                     AKSLVGHDLPSALLHAGDRTRT"
     gene            1782..3020
                     /locus_tag="VFI55_11545"
     CDS             1782..3020
                     /locus_tag="VFI55_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7074701.1"
                     /translation="MDFTLPEHLPGLLAEMDEFIEAEIKPLERGHMQYFDQRREHART
                     DWDNDGVPSREWEELLAEMRRRADEAGWLRYGLPASLGGRDGTNLDMAVIRQHLAHKG
                     LGLHNDLQNESSIVGNFPQVIMMERFGTGAQRRLWSEALITGERSMAFGLTEPRHGSD
                     ATWLETHAEPDGDGWVINGAKRFNTGVHRATHDLIFARTSGEPGAAVGITAFLVPTDA
                     PGFKVPFYWWTFNMPTDHGEVELNDVRVPGDSVLGEVDRGLEVAQTFLHENRIRQAAS
                     SLGAAQYCIDRAADYARERTVFGKPLSVNQAVQWPLAELATEAQMVRLLVQYAAWHLD
                     RDHHMEVSDKVSMANYRANRLVCDAADRAMQIFGGLGYSRHEPFEHIYRHHRRYRITE
                     GAEEIQIRRIAQRLLKFGAK"
     gene            3044..>3274
                     /locus_tag="VFI55_11550"
     CDS             3044..>3274
                     /locus_tag="VFI55_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET7074702.1"
                     /translation="MALALPRVLTPALGAGVRIENLRALTGGASRTTWAFDAVTGEQH
                     RSLILRTGPPDDVHAGMELEARAQAAAAATGAP"
BASE COUNT          545 a         1136 c         1108 g          485 t
ORIGIN      
        1 atcatcctgg tccgggtgtc gggctacggg cagaccgggc cggatgcaca caaggccggc
       61 tacgcctcgg tggccgaggc cgccagcggg ctgcgtcacc tcaacggctt tcccggcggg
      121 cccccgcccc ggctggcact ctcgctcggc gacagcctgg ccggcatgtt cggcgcgcag
      181 ggcgcgctgg ccgcgctgta tcgccgcagc gtcaccgggc acggccaggt ggtcgacgtc
      241 gcgctgaccg aatcgtgttt ggcggtgcag gaatccacga ttcccgacta cgccgtcggc
      301 ggagtagtgc gcggaccgtc gggcacccgg ctggacggca tcgcgccgtc caacatctac
      361 cgcagcgccg acggctcctg gctggtgatc gccgccaacc aggacaccgt gttcgcccgg
      421 ctgtgcaagg cgatggggcg tcccgagctg gccaccgacg accggttcgc cacccacggc
      481 gcccgtggcc gcaaccaaga cgaactcgac acgatcatcg gcgcgtgggc cgccgagcga
      541 caacccggcg acatcatcga aacactttcc gccgcagggg tgatcgcggg gccgatcaac
      601 accgtcgccg aggtggtcaa cgatccccag cttcgtgcgc gcggcatgct ggtcgaacac
      661 tacgacgaac gagtcggctg cgatgtcctg ggtccgggcg tcgtgccggt gctctccgag
      721 tcccccggcc gtgtccgcca cgccggaccg gcacggcccg ggcagcacaa cgaggacgtc
      781 tacgtcgggc tgctgggaaa gaccaccgag gaactcggcg aattgcgcgc cgagggggtg
      841 ttatgagccc ccacgtcgat attcgcgagg tgtcgctgcg cgacggcctg cagatcgaga
      901 agccaatccc cttgacggcc aagctggaac tgctggctgc ggtggccgcc accggggtgc
      961 gcgaagtgga ggccaccgca ttcgtctccc cctccaaggt gcccgcgatg gccgacgcgg
     1021 ccgagctcgc cgcgcagctg cacgactacc ccgacatcga gttctcggcc ctggtggccg
     1081 gtcccaacgg cgccaagcgc gccgccgccg ccggactgcg atcgatcgag tacgtggtgg
     1141 ccgccagcga cgcgttcagc agcgccaacg tcgggcgcaa cagtgccgag gccaccgacc
     1201 agatcgagga gatcgtcgcc atcgcgcacg acagcaacat gagcgttgaa gtcatcgtcg
     1261 ccacggcctg ggactgcccg ttccaagggc cgaccccgcc ggggcgggta ctcgagatcg
     1321 gcgcggccgc ccgcgatcgg ggcgtcgaca ggttctcgat cgccgacacc atcggcacca
     1381 ccaccccgaa acgggtgagt tcgctgatcg cacaactgcg tccggtaatc ggagacatgc
     1441 cgctgggcgg gcatttccac aacacccgcg gcgccgggct ggccagcgcc tacgccgcgg
     1501 tctgctccgg ggtcacccgg ctggacgcct cggtcggcgg gctgggcggc tgcccgttcg
     1561 cacccggcgc caccggcaac atcgccaccg aggacctggt gtacctgttg cgggacagcg
     1621 acatcgacgt cgacatcgac ctgtctgccg ccatcgccgc cgcgcgagtc gccaaatcgc
     1681 ttgtcggcca tgatcttccg agtgcactgt tgcatgccgg cgaccggact cggacctgaa
     1741 agctctgtac ggcggcgtaa cgagatcttg aggagataat tgtggatttc actctcccag
     1801 aacatcttcc gggcctgctc gccgagatgg acgagttcat cgaggccgaa atcaagccgc
     1861 tggaacgcgg ccacatgcaa tatttcgacc agcgccgaga acatgcccgc accgattggg
     1921 acaacgacgg cgtcccgtcg cgcgaatggg aggagctgct cgccgagatg cgccgacggg
     1981 ccgatgaggc gggctggctg cgctatgggc tgccggcgtc gttgggtggc cgggacggca
     2041 ccaacctcga catggccgtc attcgccaac atttggcgca caaaggcctt ggcctgcaca
     2101 acgacctgca gaacgagtcg tcgatcgtcg ggaattttcc gcaggtgatc atgatggaac
     2161 gcttcggcac cggcgcgcag cgtcgccttt ggtcggaggc gctgatcacc ggggaacggt
     2221 ccatggcgtt cggccttacc gagccgcggc atggctccga cgccacctgg ctggaaacac
     2281 acgccgaacc cgacggtgac ggctgggtga tcaacggcgc caagcggttc aacaccgggg
     2341 tgcaccgagc cacccacgac ttgatcttcg cccgcacctc cggcgagccg ggagcggcgg
     2401 tgggcatcac cgccttcctg gtcccgacgg atgcgcccgg attcaaggtc cccttctact
     2461 ggtggacgtt caacatgccg accgatcacg gcgaggtcga gctgaacgac gtgcgggtac
     2521 ccggcgattc ggtgctcggt gaggtggacc gcggcctgga ggtcgcacag actttcctgc
     2581 acgagaaccg gattcggcag gcggcaagca gtctgggcgc ggcgcaatac tgcatcgatc
     2641 gtgccgccga ctatgcccgc gagcgcacgg tgttcggcaa gccgctatcg gtcaaccagg
     2701 ccgtgcaatg gccactggcc gagctggcta ccgaagccca gatggtgcgg ctgctggtgc
     2761 agtacgcggc ctggcatctg gaccgtgacc accacatgga ggtctccgac aaggtgtcca
     2821 tggccaatta ccgcgccaat cgactggtct gcgacgccgc cgaccgggcc atgcagatct
     2881 tcggcgggct cggttacagc cgtcacgagc cgttcgagca catctaccgc catcaccgcc
     2941 gctaccggat caccgagggc gccgaggaga ttcagattcg ccggatagcg cagcggctgt
     3001 tgaagttcgg cgcgaagtga cggctgtcct gggcccggcc gagctggcgc tcgccctacc
     3061 gcgggtgctg acgccggcgc tcggcgccgg cgtcaggatc gagaatctgc gcgcgttgac
     3121 cggcggcgcc agccgcacca cgtgggcgtt cgacgccgtc acgggcgagc agcaccgatc
     3181 gttgatcctg cgcacgggtc cgcccgacga cgtgcacgcc ggcatggaac tcgaagcccg
     3241 cgcgcaggcc gcggccgcgg cgaccggagc gccg
//
LOCUS       DASSGQ010000261         2882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_929826,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000261 DASSGQ010000000
VERSION     DASSGQ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929826"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VFI55_11555"
     CDS             complement(<1..497)
                     /locus_tag="VFI55_11555"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7074703.1"
                     /translation="MLTVDFDRLGIGPSSKVIDVGCGAGRHAFEAYRRGADVIAFDHD
                     ESELRSVAAILRAMAEQGEAPAAASATVVLGDALNMPYADETFDCVIASEILEHIPHD
                     DVAIAELVRVLKVDGTLAISVPRWLPERVCWLLSDEYHSNEGGHVRIYRAGELRDKIA
                     GGGMD"
     gene            complement(491..1747)
                     /locus_tag="VFI55_11560"
     CDS             complement(491..1747)
                     /locus_tag="VFI55_11560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7074704.1"
                     /translation="MRIALLSYRSKEHCGGQGVYVRHLSRGLVELGHDVEVFSGQPYP
                     ELLDPRVRLTKVPSLDLYREPDPFRVPRPSEIRDWIDLLELLTVWTAGFPEPRTFTLR
                     VARLLAERADDFDLVHDNQSLGTGLLTIADAGLPVVATVHHPITRDRVLDLAAARWWR
                     KPLVRRWYGFSTMQERVARQIPDLLTVSSSSAADIVTDFGVTPDQLHVVPLGVNTDLF
                     RPGSQPRQPGRIIAIASADTPLKGVGTLLRAVARLRSRRDVELRLVAKVEANGPTHKL
                     IAELGISDIVHITSGLPDAELARLFASAEVACIPSLYEGFSLPAVEAMASGTPIVASR
                     VGALPEVLGTDGACAELVPAADVDELTRALGELLDSPEKRRGMGQAGRSRALDVFSWE
                     AVAAQTARVYERGIARRAALEGGPQC"
     gene            2061..2858
                     /locus_tag="VFI55_11565"
     CDS             2061..2858
                     /locus_tag="VFI55_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HET7074705.1"
                     /translation="MHDSAAPRGPRSVSRRDVLKFGAAPILLSLGAVAAPIGGRQAAA
                     ADMQLIDFAERRIAPDEIKSAGYGGVVNYVSESRPGANFEAKPITREYADALRAAGLQ
                     IVSNFQYGKPGWPDPSDYTRGHDGGVADAQTAQRLHAAAGGGGSAPIFFSVDDDIDAD
                     TWNGTAVDWFRGINSVLGVGRTGIYGHARACGWAIRDGVIGNSSTPGHQWAWQTKSWS
                     HGEREPAAVLYQAVVNTASNPGPLLGGINVDVDDVLVADFGQWDFPR"
BASE COUNT          485 a          956 c          983 g          458 t
ORIGIN      
        1 aaatccattc cgccgccggc aatcttgtcg cgcaattcgc cggcccggta gatgcgcacg
       61 tggccgcctt cgttgctgtg gtattcgtcg gaaagcagcc agcacacccg ctcgggaagc
      121 catcgcggca cgctgatcgc cagtgtgcca tcgactttga gcaccctgac cagttcggcg
      181 atggcgacgt cgtcgtgggg gatgtgttcc agaatttccg aggcgatgac acagtcgaat
      241 gtctcgtcgg cgtacggcat gttcagcgcg tcaccgagaa ccacggtcgc cgacgccgcg
      301 gcgggcgcct cgccctgctc ggccatcgca cgcagaatcg cagcgacgga acgcagttcg
      361 gattcgtcgt ggtcgaaggc aatgacgtcg gcgccacgtc gatacgcttc gaaggcgtgc
      421 cggcccgctc cgcacccgac atcgatgacc ttgctggacg gcccgatacc gagccggtcg
      481 aaatcgacgg tcagcattgc ggcccaccct cgagggcggc ccggcgggcg atcccccgct
      541 cgtagacccg tgcggtttga gcggccacgg cttcccaact gaacacgtcg agcgcgcggc
      601 ttcggcccgc ctgccccatg ccgcggcgct tttcgggcga gtcgagaagc tcaccgagcg
      661 cgcgggtcag ctcgtcgaca tcggcggcag gcaccagctc agcgcaggca ccgtcggtcc
      721 ccaaaacttc ggggagtgca cccacccggc tggcgacgat cggggttccg ctggccatgg
      781 cttccaccgc gggcagcgaa aacccttcgt agagagaggg tatgcacgcg acctcggccg
      841 aggcgaacaa tcgggccagc tctgcgtcgg gaagtccgct ggtgatgtgc acgatgtcgg
      901 agatgccgag ttcggcgatg agcttgtggg tggggccgtt cgcttcgacc ttggcgacga
      961 gccgcagctc gacatcgcgc ctgctgcgta gcctggccac cgcccgcagc agggtcccga
     1021 cgcccttcag cggggtatcg gcgctggcga tcgcgatgat tcgaccgggt tggcgtggtt
     1081 gcgaacccgg ccggaagagg tcggtgttca cccccaacgg caccacgtgg agttgatccg
     1141 gggtgacccc gaagtcggtg acgatgtccg cggccgacga cgacgagacg gtcaacaggt
     1201 cggggatctg gcgcgccacc cgctcctgca tcgtggaaaa tccataccaa cggcgcacca
     1261 acggttttcg ccaccatcgc gcggcggcga gatccagcac ccggtcgcgg gtgatgggat
     1321 ggtgcacggt ggccaccacc ggcagccccg cgtcggcgat ggtgagcagg ccggttccca
     1381 ggctctggtt gtcgtggacg aggtcgaaat cgtccgcccg ctcggccagt agccgcgcga
     1441 cacgcagcgt gaaggtgcgc ggttcgggaa agccggccgt ccacaccgtc agcagttcca
     1501 gcaggtcgat ccagtcccgg atctcgcttg ggcggggcac ccggaacggg tctggctcgc
     1561 ggtagaggtc gaggctgggc accttggtca gccgcacccg cggatccagc agttccgggt
     1621 agggctgacc ggagaacacc tcgacgtcgt ggccgagttc gaccaggccg cggctcagat
     1681 ggcgcacgta gacgccctgc ccgccgcaat gctccttgct gcgataggac agcagggcaa
     1741 ttcgcatact caactcttat ccgctcttat ccgctcttat ccgcagatgc aagaccgcga
     1801 acggccaccc ccagtccatt acggtcaacg agctcccggg ctccgcgacc caaaactgga
     1861 catgtgtcca gactatagtt cgatcaccta acggacgcaa cgagctggca cgccgacgta
     1921 gcggcccggg ctcgcgggtg atcgcccgac gcagtggtca ctacaggttg tcgctccgag
     1981 gcgggcagat tcgatatcgc gggacgtcgc gtccggcaga ggcgtaggtg accggcaccg
     2041 aagcttgtta ccatcgcgcg gtgcacgatt cggctgcgcc acgtggaccg cgatcggttt
     2101 cccggcgcga cgtcctcaaa ttcggtgctg cgccgattct cctgagcctc ggggcggtgg
     2161 ccgcaccgat cggcgggcgg caggcagcgg ccgccgacat gcagctgatc gattttgccg
     2221 agcgcaggat cgcaccggac gagatcaaat cggcgggcta cgggggagtg gtcaactacg
     2281 tgtccgagtc gcggccgggc gcgaacttcg aggccaagcc catcacgcgc gagtacgccg
     2341 acgcgctgcg ggcggccggc cttcagatcg tcagcaactt ccagtacggc aaacccggct
     2401 ggcccgaccc gtcggactac acccgcgggc acgacggcgg cgtggcggac gcccagacgg
     2461 cgcagcggct gcacgccgcg gccgggggtg ggggttcggc gccgatcttc ttcagcgtcg
     2521 atgacgacat cgacgctgac acctggaacg gcaccgccgt cgactggttc cgggggatca
     2581 actcggtctt gggcgtgggc cgcaccggca tctatgggca tgccagggcg tgcgggtggg
     2641 cgatcagaga cggcgtcata ggaaactcga gcacccccgg gcatcaatgg gcgtggcaga
     2701 cgaagtcctg gtcgcatggg gagcgcgaac ccgcggcggt gctgtatcag gccgtggtca
     2761 acaccgcgtc gaacccgggt ccgctgctgg gcgggatcaa cgtcgacgtc gacgacgtcc
     2821 ttgtggccga tttcggccag tgggattttc cccgctgatc caacgacacc ccgcgagccg
     2881 gt
//
LOCUS       DASSGQ010000262         6228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1173068,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000262 DASSGQ010000000
VERSION     DASSGQ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173068"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VFI55_11570"
     CDS             <1..585
                     /locus_tag="VFI55_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HET7074706.1"
                     /translation="HVFGSRLAVTHDAGAAAFGAGPDGWDGPGFFGPLSQPLPMSLRQ
                     HPRWGEFYADERLAPMCELAAARLDASTRAAIDAVAARCRAGDFDDDDPPARLHGDLW
                     SGNVMWTPAGVVLIDPAAHGGHRETDLAMLALFGCSHLDAVLGGYQRVRSLKPGWRNR
                     VGLHQLFPLLAHVVLFGGGYARQTNAAARAALAA"
     gene            725..1552
                     /locus_tag="VFI55_11575"
     CDS             725..1552
                     /locus_tag="VFI55_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7074707.1"
                     /translation="MTIDVWMQHPTHRFLRSDMLASLRRWTGGSIPDSEVPIEATVAS
                     MDAAGVDFGLLSAWRGPGGQDLVSNDEVAEWIRLYPSRFAGLATVDLDRPMAAVRELR
                     GRVREGGFVGLRVVPWLWDAPPTDRRYYPLFAECVESGVPFCTQVGHTGPLRPSETGR
                     PIPYIDQVALDFPELVIVCGHVGYPWTEEMVAVARKHENVYIDTSAYTIRRLPQELTR
                     FMKTGTGQRKVLFGTNYPMMTHRHALAGLDELGLSDQARRDFLHRNAEGVFGLEAIR"
     gene            1549..3099
                     /locus_tag="VFI55_11580"
     CDS             1549..3099
                     /locus_tag="VFI55_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HET7074708.1"
                     /translation="MNGAQALINTLVDGGVDVCFANPGTSEMHFVAALDTVPQMRGVL
                     ALFEGVATGAADGYARIADRPAAVLLHLGPGLGNGLANLHNARRARVPMVVVVGDHAT
                     YHKKYDAPLESDIDALAGSVSGWMRRTEHTADVATDAAEAIVASLSGSQISTLILPAD
                     VSWSDGAHPASTVPAHPASADPQLARGVLEVLRSGEPTVLMVGGDATRGRGLAAAARI
                     AQATGARLLCETFPTRLERGAGVPAVERLAYFAEAATSQLDGAKHLVLAGAKSPVSFF
                     AYPGMPSDLVPAGCEVHVLAAHAGAADALAALADEVAPGTVAPLAEASRPQLPTGALT
                     SASAADVIGALMPERTIVVDESNTSGLLLAQATAGAPAHDWLTLTGGAIGYGIPAAVG
                     AAVAAPDRPVLCLESDGSAMYTISGLWSQAREKLDVTTVLYDNSAYDILRVELQRVGA
                     GSAPGPNALDLLDLSRPTMDFVKIAEGMGVPARRVTTAEEFADTLRAAFAEPGPHLID
                     AVVPSLFG"
     gene            complement(3088..3792)
                     /locus_tag="VFI55_11585"
     CDS             complement(3088..3792)
                     /locus_tag="VFI55_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074709.1"
                     /translation="MTLPRPNLEQSFEELAAAVPATIGVAISGPDRTEPRALGRWSSG
                     VAWSTIKVPLAIAALRRDRPGAEDLVVKAITESDNDASERLWTQLGAPADAAQQVQAV
                     IDESGDTATVVESHRLRPGFTAFGQTQWALARQARFAAQLPYFDHGATVVDLMRQLAS
                     HQRWGLAAKGLAAKGGWGPGIRGDYLVRQFGIIPVEFGQVGVALAAQADAFETGVYVL
                     NTMTDWVVSHLRVLTE"
     gene            complement(3848..5356)
                     /gene="fadD17"
                     /locus_tag="VFI55_11590"
     CDS             complement(3848..5356)
                     /gene="fadD17"
                     /locus_tag="VFI55_11590"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD17"
                     /protein_id="HET7074710.1"
                     /translation="MIPTDPTVTGLLAPLAEIVDRGVYFEDSFTCWRDHVRHGAAIAA
                     TLRDRLDPAKPPHVGVLLQNTPFFSAMLVAAGMSGIVPVGLNPVRRGQALARDVANAD
                     CQLVLTDSKSAAPLDDIEHLNVDSADWADELAAHRDAEPHFRPASPADLFMLMFTSGT
                     SGEPKAVKCSHGKVAIAGVTMTQRFDLDRHDVCYVSMPLFHSNAVLVGWAVAAACQGS
                     MVLRRKFSASNFLIDVRRYGVTYANYVGKSLSYLLATPERPDDADTPLRAVYGNEGVP
                     ADVDRFGRRFGCVVQDGFGSTEGGVAIVRTPDTPQGALGPLPEGIDIVDPDTGIPCPA
                     GVVGELVNTAGPGRFEGYYNDEAAEAERMAGGVYHSGDLAYRDEAGYAYFAGRLGDWM
                     RVDGENLGTAPIERVLLRYPDATEVAVYAVPDPVVGDQVMAALVMTPGAEFNPAKFRA
                     FLADQPDLGPKQWPSYVRIGAELPRTVTFKVLKRQLSAQGVDCADPVWPIRR"
     gene            complement(5362..>6228)
                     /locus_tag="VFI55_11595"
     CDS             complement(5362..>6228)
                     /locus_tag="VFI55_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET7074711.1"
                     /translation="AVPYLESVVLAAGALARFGSEDLQQTWGVPAIHGEKVLTVALDG
                     DMGEGPVQATRAGDGYRLTGTRTQVGFGPVADAILVPAETDSGTAVFLVAADDPGVTV
                     TAMETTGLGSVGHLALDGTEVDGGRKVSDATDGTDRRVAAWLGTLATLGRSAFQLGVL
                     ERGLQMTAEYARTREQFDRPIGSFQAVAQRLADGYIDVKGLRLTLTQAAWRVSEDIPA
                     EIDVASAAFWAADAGHRVAHTIVHVHGGVGVDTDHPVHRYFLAAKETEFALGGATSQL
                     RRIGRELAETPA"
BASE COUNT          955 a         2139 c         2123 g         1011 t
ORIGIN      
        1 cacgtgttcg gcagccggct ggccgtcacg cacgacgcgg gtgccgcggc gttcggcgca
       61 gggcccgacg ggtgggacgg gccggggttc ttcgggccgt tatcgcagcc attgcccatg
      121 tcgttgcggc aacacccacg ctggggcgag ttctacgccg acgagcggct ggcccccatg
      181 tgcgagcttg cggcggcgcg gctcgatgcg tcgaccaggg cagcgatcga cgccgtcgcg
      241 gcgcgatgcc gtgccggcga cttcgacgac gacgacccgc cggctcggct gcacggcgat
      301 ctgtggagcg gcaacgtgat gtggacgccg gccggagtgg tgttgatcga cccggcggcg
      361 cacggcggtc accgcgagac cgacctcgcg atgctcgcgc tgttcggctg ctcgcacctt
      421 gacgccgtcc tcggtggtta ccagcgggtc cggtcgctga aacccggctg gcgcaaccgg
      481 gtcgggctgc accagctctt cccgctgctg gcgcatgtgg tgctgttcgg cggtgggtac
      541 gcacggcaga cgaatgccgc cgcgcgggcc gcactggcgg cctgacctcc gtctagagtt
      601 ggattgcccg ctcctcctcc cgccgcttcg cggcgggcat cgtcggcggc tcgtgtcgga
      661 ttgcccgcct cctcctcccg ccgcttcacg gcgggcatcg tcggcgggct agtgtcggaa
      721 cgcgatgacg atcgatgtct ggatgcagca cccgacgcac aggttcctgc gcagcgatat
      781 gttggcctcg ctgcggcgct ggacgggcgg gtcgattccc gactccgaag ttccgatcga
      841 ggcgaccgtt gcgtcgatgg acgcggccgg ggtcgacttc ggcctgctga gtgcgtggcg
      901 cggcccgggc ggccaggact tggtatccaa cgacgaggtc gcggaatgga tccggctgta
      961 cccgagccgg ttcgccggtc tggccacggt cgacctcgac cggccgatgg ccgccgtccg
     1021 cgagctgcgc ggccgggtgc gcgagggcgg attcgtgggt ctgcgggtgg tgccctggct
     1081 gtgggacgcg ccgcccaccg accggcgcta ctacccgttg ttcgccgaat gtgtggagtc
     1141 cggcgtgccg ttctgcaccc aggtcggaca taccggtcca ctgcggccat cggagactgg
     1201 gcgcccgatt ccctacatcg atcaggtggc cctcgacttt cccgaactcg tcatcgtctg
     1261 cggccacgtc ggctatccat ggaccgagga gatggtcgcg gtcgcccgca agcacgagaa
     1321 cgtctacatc gacacgtcgg cctacacgat caggcgactg ccacaagagc tcactcggtt
     1381 catgaaaacc ggtaccggac aacgcaaagt cttgttcggc accaactacc cgatgatgac
     1441 ccaccggcac gcgttggcgg ggctcgatga actcgggctc agcgatcagg cccgccgaga
     1501 ctttctgcac cgcaacgccg agggtgtttt cggactggag gcaatcaggt gaacggcgct
     1561 caggccctga tcaacaccct ggtcgacggc ggcgtcgacg tgtgtttcgc caaccccggc
     1621 acgtccgaga tgcacttcgt ggccgccctg gacaccgttc cgcaaatgcg tggcgtgcta
     1681 gcgcttttcg agggcgtggc caccggcgcc gccgacggct atgcccgcat tgccgatcgg
     1741 ccggccgcgg tgctgctgca cctgggcccc ggattgggta acggccttgc caacctgcac
     1801 aatgcgcgcc gcgcccgggt gccgatggtg gttgtcgttg gcgaccacgc cacctatcac
     1861 aaaaagtacg acgccccact ggaatccgac atcgacgcgc tggccggcag cgtctcggga
     1921 tggatgcgcc gcaccgagca caccgccgac gtcgccaccg acgccgccga ggcgatcgtc
     1981 gccagcctgt ccggttcgca gatctcaacg ttgattctgc ccgccgacgt gtcctggtcc
     2041 gacggcgcgc atcccgcttc cacggtgccc gcgcaccctg catccgccga cccgcaattg
     2101 gcgcgcgggg tactcgaggt gttgcgctcc ggcgaaccga cggtgctcat ggtcggcggg
     2161 gacgcgaccc gtggacgagg gctggccgcc gccgcgcgga tcgcccaggc gacgggggcc
     2221 cggctgttgt gcgagacgtt cccgacgcgg ctggaacgcg gcgcgggcgt gcccgccgtc
     2281 gagcggctcg cctatttcgc cgaggccgcg acgtcccagc tggacggtgc caagcatctg
     2341 gtgctggcgg gcgccaagtc gccggtgtcc ttcttcgcct acccgggcat gcccagcgac
     2401 ctggtgccgg ccggatgcga ggtgcacgtg cttgccgcgc acgctggtgc ggccgacgcg
     2461 cttgcggcgt tggccgacga ggtggcgccc gggacggttg cgccgctggc cgaagcgtcg
     2521 cgtccgcagc tgccgacggg tgccctgacg tccgcgtcgg ctgccgacgt catcggagcg
     2581 ttgatgcccg aacggacgat cgtcgtcgac gagtcgaaca cgtccgggct gctgctggcc
     2641 caggccaccg ccggtgcgcc ggcccacgat tggctgaccc tgaccggggg agcgatcggc
     2701 tacggcattc ccgcggctgt gggtgccgcg gtggcagcac cggatcgtcc ggtgctgtgt
     2761 ctggagtccg acgggtctgc gatgtacacg atttcggggc tgtggagcca agcgcgcgag
     2821 aaactcgatg tgaccaccgt gctctacgac aacagcgcct acgacatttt gcgagttgag
     2881 ctacaacgcg tcggtgccgg ctcggctccc ggccccaatg cgctcgatct cctcgattta
     2941 tcccgcccca caatggattt cgtcaagatc gccgagggta tgggagttcc ggcgcggcgc
     3001 gtcaccaccg cggaggaatt cgccgacacc ctgcgcgcag ctttcgccga accaggaccg
     3061 cacctgatcg atgctgtggt gccgtcacta ttcggttagc acgcgaaggt ggctgacgac
     3121 ccagtccgtc atcgtgttga ggacgtacac gccggtctcg aacgcgtcgg cttgggccgc
     3181 caacgccaca cctacctgcc cgaactccac cggaatgatt ccgaattggc ggaccagata
     3241 atcgccgcga atccctggcc cccaaccacc tttggcggca aggcctttag cggccaggcc
     3301 ccagcgctgg tggctggcga gttggcgcat caggtcgacg acggttgccc cgtggtcgaa
     3361 gtacggcagc tgcgcggcga atcgcgcctg ccgagccagg gcccattgcg tttggccaaa
     3421 cgcggtgaag ccaggccgaa gtcggtgtga ctcgaccacg gtggccgtat caccggattc
     3481 gtcgatgacg gcttgcacct gttgggcggc gtccgccggc gcacccagtt gggtccacaa
     3541 tcgctcggac gcgtcgttgt cggattcggt gatggctttg acgaccaagt cttcggcgcc
     3601 cggccgatcg cgacgcaagg cggctatcgc cagaggcact ttgatcgtgg accacgcgac
     3661 accggacgac cagcggccaa gcgcacgcgg ctcggtccga tccggtccag agatcgcgac
     3721 accgatggtt gccgggacgg cggcggcaag ctcttcgaag ctttgttcga ggttgggccg
     3781 tggaagggtc atgtcctgcc tggttgcgat tcgaagtaat ccgttgggag gggtcgcccc
     3841 ggccagacta gcggcgaatc ggccacaccg gatcggcgca gtcgacgccc tgcgctgaca
     3901 gctggcgctt gagcaccttg aaggtcaccg tgcgcggcaa ctccgcgccg attcggacgt
     3961 acgacggcca ctgcttgggc cccaagtcgg gctgatcggc taggaaggcc cggaatttgg
     4021 ccggattgaa ctcagcgccc ggcgtcatga ccaatgcggc catcacctgg tcgccgacaa
     4081 ccgggtcggg caccgcgtac accgctacct cggtggcgtc gggatagcgc agcagcaccc
     4141 gctcgatggg ggccgtgccg agattctcgc cgtcgacccg catccaatca ccaagccgcc
     4201 cagcaaagta cgcatagccg gcctcgtcac gataggcgag gtcgccgctg tggtagacgc
     4261 cgccggccat ccgctcagcc tcggcggcct cgtcgttgta gtaaccctcg aaacggcccg
     4321 gaccggcggt gttcaccagc tcgcccacca cccccgcagg gcacggaatg cccgtgtcgg
     4381 ggtccacgat gtcgatcccc tcaggcagcg gacccaatgc accctgcggg gtatcggggg
     4441 tccggacgat cgccaccccg ccctcggtcg aaccgaaccc gtcctggacg acgcaaccga
     4501 atctgcggcc gaaccggtcg acgtcggcag ggacgccttc gttgccgtac accgcgcgca
     4561 gcggggtgtc cgcgtcgtcg ggccgctcgg gcgtggcgag caggtaggac aacgacttgc
     4621 ccacgtagtt ggcgtaggtg acaccgtagc ggcggacatc gatcaagaag ttcgacgcag
     4681 agaacttgcg ccgcaacacc attgaaccct ggcacgccgc ggccaccgcc cagccgacca
     4741 gcaccgcgtt ggaatgaaac aacggcatcg agacgtagca gacgtcgtgg cggtcgaggt
     4801 cgaaccgctg tgtcattgtc acgccggcga tcgcgacctt gccgtggctg cacttcacgg
     4861 ccttcggctc gccgctggtg cccgacgtga acatcagcat gaacaggtcg gcgggcgacg
     4921 cgggccggaa atgcggctcg gcatcacgat gtgcggccag ctcgtcggcc cagtcggccg
     4981 aatcgacgtt cagatgttcg atatcgtcca gtggggcagc agatttcgag tcagtcagca
     5041 ccagttggca gtcggcgttg gcgacatcgc gggccagcgc ttggccgcgg cgcaccggat
     5101 tgagccccac cggcacgatt ccggacatcc cggccgccac caacatcgcc gaaaagaagg
     5161 gcgtgttctg cagcagcacc ccgacgtgcg gcggctttgc cggatcgagg cgatcacgca
     5221 gggttgccgc gatcgcggcg ccgtgccgga cgtggtcacg ccagcaggtg aacgagtctt
     5281 cgaaatagac accccggtca acgatctcgg ccagcggcgc caacagcccg gtgacggtcg
     5341 ggtcggtcgg aatcacgtcg gctaggcggg tgtttcggcc agttcgcggc cgatccggcg
     5401 cagctggctg gtggcgccgc ccaatgcgaa ctcggtctcc ttggctgcca ggaagtaacg
     5461 gtgtaccggg tgatcggtgt cgacgccgac accgccgtgc acgtgcacga tggtgtgcgc
     5521 cacccggtgg ccggcgtcgg cggcccagaa cgccgcgctc gctacgtcga tttcggccgg
     5581 aatgtcttcc gagactcgcc atgccgcttg ggtgagtgtg agccgcaacc ctttgacatc
     5641 gatgtagcca tcggccagtc gctgcgccac cgcctggaag ctgccgatcg ggcggtcgaa
     5701 ctgctcgcgg gtgcgggcgt attcggccgt catctgcaac ccgcgctcta gcacaccgag
     5761 ctgaaaggcg ctgcggccca gggttgcaag cgtgccaagc caggcagcga ctctgcggtc
     5821 agtgccgtcg gtggcgtcgc tgaccttccg cccgccgtcc acctcggtcc cgtccagcgc
     5881 cagatgcccg acgctgccca agccggtcgt ctccatcgct gtcaccgtga cgcccgggtc
     5941 gtcggcagca accaggaaaa ccgccgtgcc ggaatctgtt tcggccggaa ccaggatagc
     6001 gtcggcgacc gggccgaagc cgacctgggt gcgggtgccg gtcagccggt agccgtcacc
     6061 ggcacgggtg gcctggaccg gcccctcgcc catgtcgccg tcgagcgcga cggtgaggac
     6121 cttctcgccg tggatcgccg gcacacccca cgtctgctgc agatcctcgg agccgaaccg
     6181 ggccagcgcc ccggcggcca gcaccaccga ctccaggtac ggcaccgc
//
LOCUS       DASSGQ010000263        13613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1416337,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000263 DASSGQ010000000
VERSION     DASSGQ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13613
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416337"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2000)
                     /locus_tag="VFI55_11600"
     CDS             complement(<1..2000)
                     /locus_tag="VFI55_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7074712.1"
                     /translation="MSSEQPQPTIDDLLDRAFDAISRGERTTAKSLAEQVLAIDRGNV
                     DAEGLLSAPSEQGEIRRLTIMFADLVDSTALSTRVEPETYRAAVGRYKEQVRQIVDRY
                     EGHVASTKGDGLLIVFGHPLAHEDDVRRALHAGLDIAQDVAALSERIRQRFGFTIQVR
                     VGVHRGVVYLDLEQDDVYGLAANLAARVSGLAPPGGVVVSAAVERLTRQHFELRRLEP
                     QPVKGIEGPIEHYLVLGERAAGARIPFRPVVGRERELAHIEHSWAQARAGTLNTVGLA
                     LRGEAGIGKSRLAAAAVELAEQSNSVVIELSGSPLHTDVGLHPVRRLLEQRCGIDRET
                     SPAELIARLETEVRARGLYSTTFVPLLAPVLAVSPQAGYQAADADGVKLYRRIARAVR
                     DYLRACLGDGPALLVVEDMHWFDEDTTEVVQSLLQEDSGRLLVVMTSRETASVSGDQH
                     VVNFVLAPLSDRESDELIVALYPELSADGRRAVCRRCDGLPLFIEEVVAKLKEQPADA
                     AESAHVPDSLYEALFARLRSRDAVRVAEGAAVVGNPIDRRLLAAAVKIRESELDAAIT
                     ELCAGRVLEPVGEETWRFRHELLRDIAAELSPPSLRRRMHGRVADALLSFTADANTDW
                     PLIASHYERAERYEAAASAYRKASGVARRRGALQEARSYLSHA"
     gene            complement(1997..2545)
                     /locus_tag="VFI55_11605"
     CDS             complement(1997..2545)
                     /locus_tag="VFI55_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074713.1"
                     /translation="MTDSRDAVQQFTATTDAAVAQARLLAQDAVAQIDAGTYGCEAWG
                     RSLVQLFDIVARGSATHFQTAVSHGCCGAVSSGSSGSCEGQCGVQPCDPIDVHEVDDN
                     FSRGLSIAAALTRVGGGVSIPPNMIGFEPAVLAAGATSFAVYLKDVQYIGASYTGTVR
                     LTRVTSDPGSVGYVDQPITVEL"
     gene            complement(2545..3999)
                     /locus_tag="VFI55_11610"
     CDS             complement(2545..3999)
                     /locus_tag="VFI55_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074714.1"
                     /translation="MTRPAETRGTLFHLLALYAALDQAVLQSRDPDIAAIVEGFLPGT
                     TGVNQRGWSIDLQNFLGREFPDVNTALSAITRAPSLTRPQLSRLVYVLGSLAHLPPSP
                     STGDELLAQGLDLARPQTQPRPPYASVTGETDAGRALLGVLSESFHTRRDWTAATTQA
                     VAMGCLDAVVASVPLCNACLAMQGGIQCVVVDTRFEDSSISIDQVKDVLDPRNWDKTA
                     GEFFCNMQGLGGHPLAPYQGWGRVLETVSAWCGSGLPVLVTDLKFLKSDNPGQAVVQY
                     DLDNPFPGGGDGQVTVDKGWLKVITGTSTDSTAGVTVTTRKVVHIDPLLPVAQKVFVC
                     VMGYGQAAMEMLLGGATNPPAGLVAWSAAPAPLVSTIAAQEFSKASQADDQTLAAGSP
                     PTAGAASAAGAAPTTAAGLAVSMLSDYLAEVTNDSSKLVAKFAKQELTVDDLAKFSAK
                     SAARMASEPWRFLTKLSELPSQPPAGGPSQGGGN"
     gene            4078..4755
                     /locus_tag="VFI55_11615"
     CDS             4078..4755
                     /locus_tag="VFI55_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074715.1"
                     /translation="MNGGVTDDGTISDLPWETVFDPAANARALSAIQARGFSAATQLV
                     NRFVRIAETSANGAGAESAPSTAEHRNGKATDADADLILTAWWSLFGRLLRSMPGAAA
                     LRSDGATFDLAGESASGGVHLNADGPGRAVGEVWLHNRGADDIDAVALRCGGLLAHDG
                     GEVGADAVRFTPDRFGLPGRSSRGVAMEVNLTPDIAPGRYRGTLLAEGYPDLWLPVTL
                     TLRAPAE"
     gene            4752..5804
                     /locus_tag="VFI55_11620"
     CDS             4752..5804
                     /locus_tag="VFI55_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET7074716.1"
                     /translation="MTSTADTTQLVVVVENRLREVAQTVRRSMLDAMPDGEPVRWLYG
                     PMREYPSRPGKALRPALCLSAGRAFGGHADDLLGIAVAIELLHNAFLVHDDIADGSQL
                     RRGRPTLAATHGVAAALNAGDGLAVVANQVLRRATRRLDPDLAELVLAEFDTMAMRTL
                     EGQATEAGWRFDEVESLGPEDYLALIMHKTCWYTTIHPLRVGALVGSGGTADLSALVR
                     FGFHLGAAFQIRDDLLNLVGDAHLYGKEILGDLYEGKRTLALMHLMEAAQGADRALLG
                     DYLRRNRSERSEELVGSIRAMMDDYGSIAFTREYAEGILLMAEEYFEQAFAQAQPGSD
                     IDFLRALVPYVWARWR"
     gene            5884..6126
                     /locus_tag="VFI55_11625"
     CDS             5884..6126
                     /locus_tag="VFI55_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1918 domain-containing protein"
                     /protein_id="HET7074717.1"
                     /translation="MKARIGDFLVVKGTTTERHDQHAEIIEVRAEDGSPPYVVRWLVT
                     GHEATVYPGSDAVVVTAAEHNEAARRAAARAGHRPK"
     gene            6264..7046
                     /locus_tag="VFI55_11630"
     CDS             6264..7046
                     /locus_tag="VFI55_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET7074718.1"
                     /translation="MSTTPTVELDRPREGVVVMRLNRPERLNAINEVMQTELSCALVD
                     LADDRSARAVVLTGAGRGFCAGIDMRDFGSDVPEATAPALDRMRFQEKMAKLAVAVRQ
                     LPQPVVAAVNGPCVGAGFALCLAADIRICSAEASFGNAAILLGLSGAEMGMSYHLPRI
                     VGTSVAADWMLTGRTVSAAEADRRGLVSELVEPERLADRAVQLASLICGLAPLGVQLT
                     KRALQVNTDAASLSSALELENRNQVIAHATDEAAARRKKWPS"
     gene            complement(7089..7775)
                     /locus_tag="VFI55_11635"
     CDS             complement(7089..7775)
                     /locus_tag="VFI55_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MspA family porin"
                     /protein_id="HET7074719.1"
                     /translation="MVVRRAAVLAVCFLMSVTLAPASGADPDVNPAAAAEAPSDGRIP
                     SGDPAVVNTPDGWRLALGARDEMQVPVAPLTTAISSREYVSSGIFVASLTGPEPPRGV
                     LEVGYEIGCGIDMSTSNGVEIGGSAGVSPGLGTTALLPGTVTGFVPLVTVPIQGSLSV
                     GLKPGLVIVVPVVKKQFKGPDPWVMISDFHVKIDGCVGQSFIRSYATLTRVTDQSDVV
                     LSYIGVTKAV"
     gene            complement(8292..9245)
                     /locus_tag="VFI55_11640"
     CDS             complement(8292..9245)
                     /locus_tag="VFI55_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET7074720.1"
                     /translation="MRMVELDLEQLRRRLAGAGITDVTELAGGASSLTFRGEHDGRAV
                     VVKVAPPGVEPVAHRDVLRQARIIKALAGSGVPVPTVVWEQTGDAPLFVMSHVEGDCV
                     EPLFDGCAPTADLRGRYRNACRAMAALHSLSPRELGLGGEAVLDPAAEVRRWCHTLRT
                     VDAALLPDWPGVSDALLACAPTAMGPSVVHGDFRLGNLIAVGEDINAVIDWEIWSIGD
                     PRIDVGWFLINCDPDTYQRVPASAGVAPPTTELAQIYQYELGREVGDLAWFCALACFK
                     SAATWSLIVKHNRRRRAPQAELEAMAATLPRLLARARSMLA"
     gene            complement(9242..10024)
                     /locus_tag="VFI55_11645"
     CDS             complement(9242..10024)
                     /locus_tag="VFI55_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7074721.1"
                     /translation="MVALVTGSSRGLGKAIAARLAAHGATVALTARTLDPDPQYQGSL
                     RQTREEIAAAGGRAVAIQADLSRSEERERLFAEVVEAVGAPDILVNDAAVTFLRPLDG
                     FPERRIRLMMEMHVLAPLHLSQLAIPAMRERGRGWILNLTSLAGDQPPGPPFSEFDRT
                     AGFGVYGTVKAALNRLTKSLAAELYDDGIAVNAAAPTNPVATPGATTLDLAKTDTEDI
                     ELITETAFRLCTGDPKTTTGRIAHTQRFLAEVGWSRTGPRTG"
     gene            10269..11009
                     /locus_tag="VFI55_11650"
     CDS             10269..11009
                     /locus_tag="VFI55_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074722.1"
                     /translation="MIGMATAARTAGGAALAGAAALGLAIGLALGPAMGTADATPGAP
                     GPASGEGMLYGDPAAAAPFWRFQEYDDDCVEMAVADVVGELTGHQPSEQAIIKLAQST
                     PSTVHSGPVYTKPKKRKPGSGTSFEDEPALLAHYGIRAVDTDKASAAKTGVPTGMNAL
                     EQDLAKGRRVIVAVNAEVIWREPVEDKTRDGEPESNHAVVVTGVDTEAGIVHLNDSGS
                     QDGRDEQVPIGIFTRSWASGDDEMTVTS"
     gene            complement(11006..11572)
                     /locus_tag="VFI55_11655"
     CDS             complement(11006..11572)
                     /locus_tag="VFI55_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET7074723.1"
                     /translation="MTTTRPRNASQTRADILSAARRRFGAEGYERATLRAIASDVGVD
                     AALVIRYFGSKQDLFAAAVDFVIELPDLSNVDPDEIAGVLLPRFFAVWEEDEKFLALL
                     RAAMTSPVAADALRRVFAEQVAPKLITATPDNPIPRIGLMGAFVIGLATTRYVLRNPP
                     IASLSRDELSRWAAPVIRQLLVGPAPTS"
     gene            complement(11620..12183)
                     /locus_tag="VFI55_11660"
     CDS             complement(11620..12183)
                     /locus_tag="VFI55_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET7074724.1"
                     /translation="MSPNRPRNAAQTRADILDAARRRFATEGFERTTLRAIAADVGVD
                     AALVIRYFGSKQDLFATATEFTIELPDLSGAEPDEIADMLLPRYFAVWEDDHSFLALL
                     QAAMTSRVAADTLNETLATHVAPVLKAATPDHAAQRIAVTDAFVIGLAATRSVLGNLP
                     VADLTRDELRRWAAPVFRGLLVGPAPT"
     gene            12405..>13613
                     /locus_tag="VFI55_11665"
     CDS             12405..>13613
                     /locus_tag="VFI55_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET7074725.1"
                     /translation="MLHRIAALAICAPRRTLALTALVMAVAGVFGAPVAQSLSASGFQ
                     DPTSESARAATILTDKFGQGEVQLLFVVSAPQGFDSPAARSVGTDIADQLSRSPHVTS
                     VTSAWTAPPAAATSLVSKDGLSGLVVAGIGGGETPSIMKAQKYADELSQQFAHDRNGV
                     TVRSGGVAMVNAQITAQAQHDVLVMEAIAIPLSFVVLVWVFGGLFTAALPVAIGGMSI
                     LASLAVLRLIAFATDVSIFALNLTTALGLALAIDYTLLIVSRFRDEMAAGATRDDALL
                     RTMATAGRTVLFSATTVALSMAAMIQFPMHFLKSFGYAGIATVAFTAIAAVVMTPAAI
                     VLLGDRLNALDVRRLVRRLAGRPRPARKPVEDEFWYRSTNFVTRRAVPVGVAVVAVLL
                     LLGAPFLGVRW"
BASE COUNT         2253 a         4541 c         4623 g         2196 t
ORIGIN      
        1 gcatggctca ggtaggaacg ggcctcctgc agcgcgcccc gccgccgggc cacgcctgaa
       61 gccttccgat aggccgacgc cgccgcctcg taccgttccg cgcgttcgta gtgactggcg
      121 atcagcggcc agtcggtgtt cgcgtcagcg gtgaacgaca gcagcgcgtc cgcgacccgg
      181 ccgtgcatcc ggcggcgaag ggagggtggc gatagttcgg ccgcgatgtc gcggaggagt
      241 tcgtgccgga atcgccaggt ctcttcaccg accggttcga gaacccgccc ggcgcacaac
      301 tccgttatcg cagcgtccag ttcggactca cggatcttga ccgccgccgc gagcaagcgt
      361 cggtcgatgg gattgccgac caccgccgcg ccctcggcca cccggacggc gtcgcgggac
      421 cgcagccggg caaacagagc ctcatacagc gagtcgggca cgtgggcgga ctccgcggcg
      481 tcggcgggct gctccttgag cttggcgacg acctcctcga tgaacagcgg taggccatcg
      541 cagcggcgac ataccgcgcg acgtccgtcg gcgctcagtt ccggatagag ggcgacgatc
      601 agttcgtcac tctcgcgatc cgacagcggg gcgagcacga agttgacaac gtgttggtca
      661 cccgagacgg acgcggtctc gcggctggtc atcaccacca gcagccggcc agagtcttcc
      721 tgcagcaatg actggacgac ctcggtggtg tcttcgtcga accagtgcat gtcttcgacc
      781 accagaagcg cggggccgtc gccgaggcag gcgcgcagat agtcacgcac cgcgcgcgca
      841 atccgccggt acagcttcac gccgtcggcg tcggccgcct ggtagccggc ctgcggggag
      901 acggcgagta cgggcgccag cagcgggacg aacgtcgtcg aataaaggcc gcgcgcccgc
      961 acctcggttt ccaggcgtgc gatgagctcg gccggggatg tctcacgatc gataccgcag
     1021 cgctgctcga gcaatcggcg caccgggtgc agcccgacgt cggtgtgcaa cggcgatccg
     1081 gacagctcga tcaccacgga attcgactgt tcggccagtt cgaccgccgc cgccgccagc
     1141 cggctcttac cgatccccgc ctcaccacgt agcgccagcc cgaccgtgtt gagtgtgccc
     1201 gccctggcct gcgcccagct gtgttcgatg tgcgcgagct cacgttcgcg gcccacgacg
     1261 ggccgaaacg gaattcgtgc gccggccgcc cgctcgccca ggacgaggta gtgctcgatg
     1321 ggcccctcga tccctttcac cggctgcggt tcgaggcgcc gcaattcgaa gtgttggcgc
     1381 gtgagccgct cgaccgcggc cgagacgacg accccgcccg gcggcgcgag acccgacacc
     1441 cgggccgcca ggttcgcagc caggccgtac acgtcgtctt gttccaggtc gaggtagacc
     1501 acgccgcgat gcacgccgac gcgcacttga atggtgaagc cgaaacgctg ccggatccgt
     1561 tcactgaggg cggcgacgtc ctgagcgatg tcgagacccg cgtgcagcgc tcgccgcacg
     1621 tcgtcctcgt gcgccagggg gtgaccgaac acgatcaaca acccgtcgcc cttggtggag
     1681 gccacgtgtc cctcgtagcg gtcgacgatc tggcggacct gctccttgta gcggccgacg
     1741 gcggcgcggt acgtctccgg ctcgaccctg gtggacaacg ccgtcgaatc gacgagatcg
     1801 gcgaacatga ttgtgaggcg gcgaatctcg ccctgttcgg agggcgcgga gagcaggccc
     1861 tcggcgtcga cgttcccgcg gtcgatggcc agcacctgct cggcgagact tttggcggtg
     1921 gttcgttctc cgcggctgat cgcgtcgaac gctcggtcaa gcaggtcgtc gatcgtcggc
     1981 tggggttgct cgctgctcac agctcgacgg tgatcggctg atcgacgtac cccacactgc
     2041 cggggtcgct cgtgaccctg gtcagccgga cggttccggt gtagctcgcg ccgatgtatt
     2101 gcacgtcttt gagataaacc gcgaaactcg ttgcgccggc cgccagcacg gcgggctcga
     2161 agccgatcat gttcggcgga atgctcaccc cgccgcccac ccgggtcaac gccgcggcga
     2221 tcgacaatcc gcgcgagaag ttgtcgtcca cctcgtgtac gtcgatcggg tcgcagggtt
     2281 ggaccccgca ttgcccttca catgaccctg atgacccgga tgacactgcc ccgcaacagc
     2341 cgtgggagac ggcggtctgg aagtgggtgg ccgaaccgcg cgccacgatg tcgaaaagct
     2401 ggaccagcga acggccccag gcttcgcacc cgtaggtgcc ggcatcgatc tgcgcgacgg
     2461 cgtcctgcgc gagcagccgg gcctgcgcaa cggccgcatc ggtggttgcg gtgaactgct
     2521 ggacggcatc tcgagaatcg gtcatcagtt gcctccgccc tggctggggc cgccggcggg
     2581 tggctgcgac ggcagctcgg aaagcttcgt caggaacctc cacggttcgc tcgccatccg
     2641 cgcggcggac ttggcgctga acttcgcgag atcgtccacc gtgagttcct gtttggcgaa
     2701 cttggccacc agcttggagc tgtcgttggt gacctcggcg aggtagtccg acagcatgct
     2761 gaccgccagc cctgccgccg tcgtcggagc cgcgccggcg gcactggctg caccggcggt
     2821 gggtggcgaa cccgccgcca gcgtttggtc atccgcctgc gacgccttcg aaaactcttg
     2881 cgccgctatc gtgctcacca gcggcgcggg agccgcggac cacgcgacca ggcccgcggg
     2941 cgggttggtc gcaccgccca acagcatttc catcgcggcc tggccgtacc ccatgacgca
     3001 gacgaaaacc ttctgggcaa cgggcagcag cggatcgatg tggacgactt tccgcgtggt
     3061 gacggtcact ccggcggtgc tgtcggtcga agtgcccgtt atgaccttca gccagccctt
     3121 gtccaccgtg acctgaccgt cgccgccgcc gggaaacggg ttgtcgaggt cgtactggac
     3181 gacggcttgg ccggggttgt ccgacttcag aaacttcaag tcggtgacca ggaccggcag
     3241 gcctgacccg caccaggcgc tcaccgtctc gaggacgcgt ccccacccct ggtacggagc
     3301 gagtggatgg ccgccgagac cctgcatgtt gcagaagaac tctccggcgg tcttgtccca
     3361 gttgcgtggg tcgaggacgt ctttgacctg gtctatcgag atcgaactgt cctcgaatcg
     3421 ggtgtcgacg acgacgcact ggatgccacc ctgcatcgcc agacacgcgt tgcacagcgg
     3481 cacgctggcg acgaccgcat cgaggcaccc catcgccact gcctgggtgg tggcggcggt
     3541 ccaatcccgc cgtgtgtgga agctctcgct gagcaccccg agcagcgcgc ggccggcatc
     3601 ggtctcaccc gtcacagagg cgtatggcgg ccgcggctgg gtctgcggcc tggcgaggtc
     3661 gagcccctgc gccaacagct cgtctccagt tgagggagac ggcgggagat gcgccaggct
     3721 gccgaggacg tacaccagcc gcgacagctg tggccgggtc aatgacggcg cccgcgtaat
     3781 ggctgacaaa gcggtattga cgtctgggaa ttcacggccc aggaagtttt gcagatcgat
     3841 cgaccagccc cgttggttca ctccggtggt accgggcaga aatccctcca cgattgcagc
     3901 gatgtccggg tccctggatt gcagcacagc ttggtcgagc gcggcgtaaa gggccagcag
     3961 gtggaataac gttccgcgtg tctccgccgg tcgtgtcatt ggccctccag cccgcgtttg
     4021 cgcagctagc ataccgtgat gcgggtatgt gcggcgtgct agcgcggagg aaatccagtg
     4081 aatggtgggg tcacggacga cggcacgatc agcgatttac cgtgggaaac ggtattcgac
     4141 ccggcagcca atgcccgggc gctgagcgca atccaggcgc ggggattttc cgcggcgact
     4201 caactcgtaa accgattcgt gcgaattgcc gaaacatcgg ccaacggcgc gggtgctgag
     4261 tccgcaccga gtacggccga gcaccgcaac ggcaaggcca ccgacgccga tgcggacctg
     4321 atcctgacgg cgtggtggtc attgttcggc cggctgctgc gctcgatgcc cggggcggcc
     4381 gccctgcgca gcgacggcgc cacgttcgac ttggcgggtg aaagcgccag cggcggtgtg
     4441 catctgaatg cggacgggcc cgggcgggcg gttggggagg tgtggctgca caaccgtggc
     4501 gccgacgaca tcgatgcggt ggcgctgcga tgcggcggtc tgctcgccca cgacggcggc
     4561 gaagtgggcg cggacgccgt gcggttcacg ccggataggt tcggcctgcc cggccggtca
     4621 agccggggag ttgccatgga agtcaacctg acacccgaca tcgcgcccgg ccgttatcgc
     4681 ggaacgctgc tcgccgaggg atatcccgat ctgtggctgc ccgtcacgct cacgctgcgg
     4741 gcgcccgccg aatgaccagc accgccgaca cgacgcaact cgttgtcgtc gtcgagaacc
     4801 ggttgcgcga ggtggcgcag accgtgcgcc ggtcgatgct cgacgcgatg cccgacggcg
     4861 agccggtccg gtggttgtac ggcccgatgc gcgagtatcc gtcacggccc ggaaaggcat
     4921 tgcggcccgc attgtgtctc tcggccggcc gcgcgttcgg agggcacgcc gacgacttgt
     4981 tgggcatcgc ggtggcgatc gagctgctgc acaacgcgtt tttggtgcac gacgacatcg
     5041 cggacggcag tcaactgcgc cgcgggcgcc ccacgctggc ggccacccac ggggtcgccg
     5101 cggcactcaa cgccggtgac ggcctggcgg tcgtggcaaa ccaggtgctg cgccgcgcca
     5161 cccggcgcct cgatccggat ctcgccgagc tggtgctggc cgagttcgac accatggcga
     5221 tgcgcacgct cgagggtcag gccaccgagg ccggctggcg gttcgacgaa gtcgagagcc
     5281 tgggcccgga ggactatctc gcgctgataa tgcacaagac ctgctggtat acgaccatcc
     5341 acccgctgcg cgtcggcgca ctggtcggtt ccggtggcac cgccgacttg agcgccctgg
     5401 tgcgcttcgg gtttcacttg ggcgcggcgt tccagatccg cgatgacctg ctgaacctgg
     5461 tcggcgacgc ccacctgtac ggcaaggaga tcctcggcga cctgtacgag gggaagcgga
     5521 cgctggcact gatgcacctg atggaggccg cgcagggcgc cgaccgtgcc ctgctgggcg
     5581 actacctgcg caggaaccga tccgagcggt ccgaggaact ggtggggtcc attcgcgcga
     5641 tgatggacga ttacggcagc atcgccttca cccgggagta cgccgagggc attctgctga
     5701 tggccgaaga gtacttcgag caggccttcg cccaggcgca gcctggatcc gacatcgact
     5761 tcctgcgcgc gttggtgccc tacgtgtggg cacgctggcg atgacttgct tccgttgagc
     5821 gacgaaagcg cgcacgatgg gaaaggtccc gcatctcaca gccgagagag aagggagcgg
     5881 gcgatgaagg ccaggatcgg cgacttcctc gttgtcaaag gcacgaccac ggaacggcat
     5941 gatcaacatg cggagatcat cgaggtgcgc gccgaagacg gctcgccacc gtacgtggtg
     6001 cgttggctgg tgaccggcca cgaggccacg gtgtatccag ggtctgacgc ggtggtggtg
     6061 accgccgccg agcacaacga ggccgcgcga cgcgctgcgg cccgggccgg ccaccggccg
     6121 aaataaccgc gcattcgcga gcactgccga caccagtcca gggtggccgg gcgcgctcgc
     6181 gaccatagct tcgccctgtc ggagaaggat attctcctga atcgagagac ccgttacgat
     6241 cggtgctgat cgaaggagac ccgatgtcga cgacaccgac cgtggaactc gaccggcccc
     6301 gcgaaggcgt tgtcgttatg cggctgaacc gcccggagcg cctcaacgcg atcaatgagg
     6361 tcatgcaaac cgaattgagc tgcgccctcg tcgatttggc cgatgaccgg tcggcgcgtg
     6421 ccgtcgtgct caccggcgcc ggtcgcggct tctgcgccgg gatcgacatg cgcgacttcg
     6481 ggtctgacgt gccggaggcc accgcgccgg cgctggaccg gatgcgcttc caggagaaaa
     6541 tggcgaaatt ggccgtggcg gtgcggcaat tgccgcaacc tgtcgtcgcc gcggtcaacg
     6601 gcccttgcgt cggcgcggga ttcgcgttgt gccttgccgc cgacatccgg atctgttcgg
     6661 cggaagcgtc gttcggtaac gccgccatcc tgctcgggct gtcgggcgcc gagatgggga
     6721 tgagctacca ccttccccgg atcgtcggaa ccagcgtcgc ggccgactgg atgctcaccg
     6781 ggcgcacggt ttcggccgcc gaagcggatc ggcgcggact ggtcagtgaa ctggtcgagc
     6841 ccgagcggtt ggccgaccgc gccgtgcagc tggcgtcgct gatctgcggc ctcgctccgc
     6901 tgggcgtgca actgaccaaa cgtgcgctac aggtcaacac cgacgcggcg agcctctcgt
     6961 cggcgctgga actggagaac cgcaaccagg tgatcgcaca cgcgaccgac gaggcggccg
     7021 cccgccgcaa gaagtggcct tcgtaaccag agacaagctg gcgggcatcc cccgcagcta
     7081 gtccgcgttc agacggcttt ggtgacgccg atgtaggaca gcaccacgtc cgattgatcg
     7141 gtcactcggg tcagcgtcgc ataggagcgg atgaacgatt gccccacgca tccgtcgatc
     7201 ttgacgtgga agtcgctgat catgacccag gggtctgggc ccttgaactg tttcttgacc
     7261 accggcacca cgattaccag gccgggcttg agacccacgc tgagcgagcc ttggatcggc
     7321 acggtaacca gcggcacgaa acccgttacc gtaccgggca acagcgccgt cgtgccgagc
     7381 cccggggaga cgccggcact accaccgatc tcgacgccgt tggacgtgct catatcgatt
     7441 ccacagccga tttcgtaacc cacctcgagc acgccacggg gcggttccgg tccggtcagc
     7501 gaggccacga atatgccgct cgagacgtat tcgcgcgacg agatcgcggt ggtcaacggg
     7561 gcgacgggaa cctgcatttc gtccctcgca ccgagggcta atctccagcc gtcgggggtg
     7621 ttgacgactg cgggatcgcc cgagggtatc cgcccgtcgg acggtgcctc tgcggcggcg
     7681 gcagggttaa cgtcgggatc ggcacccgat gcaggagcca aggtcaccga catgaggaag
     7741 cacacggcaa gaaccgcagc gcgtcgaacg accactgcta tcggtctcac gggcgacacc
     7801 gtacgcactg ccggattcgg gttgcgaagt ttgctggtcg ttccagcaaa cttgttgtga
     7861 gttcgtaact catacgcgat attggcgcgc aatgctaggg gatccgtagc gctccccggc
     7921 cgggcgagaa attctgcact cgcttcacct gccagacgcg tagaattgtc gctgccggtg
     7981 gtctccggca ataaccgctg attcggggca gacaacggag agggcggcga tgacatttcg
     8041 cacgtgcgcg cgtgggctgc tcgccggcac gacgaccctc atggttctgg cagccagcgc
     8101 cggcatcgcc cacgccgaac ccgacctcga cccgccgccc ctccccccgg tacacaacca
     8161 atgggtgcaa attccacgaa ccttcatcaa cccgtccaat gaaggccagc ccacagatga
     8221 ctcgggtgat gtgggaatga tttgcgaaaa tctattggtc gcatgccggt aaatacaggc
     8281 caattccgcg gctaggccag cattgaccgg gcacgcgcca gcaacctcgg gagagttgct
     8341 gccatggctt ccaattcggc ctgcggagcg cgccgtcgcc ggttgtgctt gacgatcagc
     8401 gaccaggtcg ccgccgactt gaagcacgcc agcgcacaga accaggccaa gtcaccgacc
     8461 tcgcggccca gctcgtactg atagatctgc gcgagttcgg tggtcggcgg tgccacaccg
     8521 gccgaggccg ggacgcgttg gtaggtgtcc ggatcgcagt tgatcaagaa ccagcccacg
     8581 tcgatacgcg ggtcaccgat cgaccagatc tcccaatcga tcaccgcgtt gatgtcctcg
     8641 ccgacggcga tcaggttgcc cagccggaag tcgccgtgca ccacactcgg ccccatagcc
     8701 gttggcgcgc aagccaacaa cgcgtcactc acccccggcc agtcgggcag cagcgccgca
     8761 tcgacggtcc gcagcgtgtg acaccagcgc cggacctcgg cggccggatc caggaccgcc
     8821 tcgccgccaa ggccgagctc gcgcggcgac agactgtgca gtgcggccat cgctcggcac
     8881 gcgttgcggt accgcccgcg caggtcagcc gtcggtgcgc agccgtcgaa aagcggctcc
     8941 acgcagtcgc cttcgacatg ggacatcaca aacaggggcg cgtctccagt ctgctcccac
     9001 accaccgtcg ggaccggaac cccgctcccc gcaagggctt tgataatgcg ggcctgtcgc
     9061 agcacatcgc ggtgcgcgac gggttcaacg ccgggcggag caaccttgac cactaccgcc
     9121 cggccgtcgt gctcaccccg gaaggtcaga ctggacgcgc ccccggcgag ttcggtgacg
     9181 tcggtgatgc cggcgcccgc gagccgtcgt cgcagctgtt ccaggtcgag ctcgaccatc
     9241 ctcatccagt ccgcgggccg gttctcgacc agccgacttc ggcaaggaag cgctgggtgt
     9301 gcgcgatgcg accggtcgtg gtcttcggat cacccgtgca caggcgaaag gcggtctcgg
     9361 tgatcagttc gatgtcctcg gtgtcggtct tggccagatc cagggtggtg gcgccggggg
     9421 tcgccacggg gttggtagga gcggcggcgt tgacggcgat gccgtcgtcg tagagttcgg
     9481 cggcaaggct tttcgtgagg cggttgagcg cggccttgac cgtcccgtag accccgaagc
     9541 ccgcggtccg gtcgaactcg gagaagggcg gccccggcgg ctggtcgccg gccagcgacg
     9601 tcaagttcag aatccagccc cggccgcgtt cgcgcatcgc gggaattgcc aactgactca
     9661 gatgcaatgg cgccagcacg tgcatctcca tcatcagcct gatccgccgt tcggggaacc
     9721 cgtccagggg tcgcaggaag gtgaccgcgg catcgttgac caggatgtcg ggggcgccga
     9781 cggcctcgac cacttcggcg aaaagccttt cacgttcctc ggatcgggac agatccgcct
     9841 gaatcgcgac ggcccggccg ccggcggccg cgatctcctc acgagtctgg cgcagcgatc
     9901 cctggtactg gggatcgggg tccagcgtcc gggcggtcag cgccaccgtg gcaccgtgcg
     9961 cggccagccg tgcggcgatc gccttaccca gccctcggct gcttccggtg actagagcca
    10021 ccatcccgtc acaccgtcgg tcagcactca acgtcgacct cctcgggact gcggaaacga
    10081 cttctccgaa ctagagaatg tcattatcgc cgatcgatcg gaagccgccg cggtgtcagc
    10141 gcttggcgct gccgcgtagc accctccgta gagaatcctc caagaatttg cagaggtaac
    10201 gaagggaacc tttttgcgga tagccacttt gtcaccagtg gcttggaccg cgtggaaaag
    10261 gacaccgcat gatcgggatg gcgaccgcgg cccggaccgc cggcggggcc gcgctggccg
    10321 gtgcggccgc gctgggactg gcgatcggcc tggcgttggg cccggcgatg ggtaccgccg
    10381 acgccactcc gggcgcaccc ggcccggcga gcggggaggg catgctgtac ggcgaccccg
    10441 cggcggccgc tccgttctgg cgcttccagg aatacgacga cgactgcgtg gaaatggccg
    10501 tcgccgacgt ggtcggtgag ctgaccggtc accagccctc cgagcaggcc attatcaagc
    10561 tggcccagtc gactccgagc accgtccact ccgggccggt ctacaccaag ccgaaaaagc
    10621 gcaagccggg cagcggcacc tcgttcgagg atgagccggc gctgctcgcg cattacggta
    10681 tccgtgccgt ggacaccgac aaggcaagcg cggcgaagac cggtgttccc acgggcatga
    10741 acgcactcga gcaggatctg gccaaggggc gcagggtgat cgtcgccgtc aacgcggaag
    10801 tcatctggcg cgagcccgtc gaggacaaaa cccgagacgg cgaacccgag tccaaccatg
    10861 ccgtcgtggt gaccggtgtc gacaccgaag ccggcatcgt tcacctcaac gacagcggca
    10921 gccaggacgg gcgggacgag caggtgccca tcggcatctt cacccgatcg tgggccagcg
    10981 gcgacgacga aatgaccgtc accagctagg aggtcggcgc cggaccgacc aatagttggc
    11041 gaatgaccgg agcggcccag cggctgagtt cgtcgcggct gaggcttgcg ataggtggat
    11101 tcctcagcac gtagcgcgtc gtggccagcc cgatgacgaa ggcgcccatc agcccgatgc
    11161 gcgggatcgg gttgtccggg gttgcggtga tcaacttggg cgcaacctgc tcggcgaaaa
    11221 cacgccgtag cgcgtcggcc gcgaccgggc ttgtcatggc ggcccgcagc aacgccagaa
    11281 acttttcgtc ctcttcccac acggcgaaga agcgcggcaa caggacgccg gcgatctcgt
    11341 cgggatcgac attcgacaag tccggcagct cgatgacgaa gtcgaccgcc gccgcgaaca
    11401 ggtcctgctt gctgccgaag tagcggatca ccagcgccgc atccacgccc acatcggatg
    11461 cgatcgcccg cagggtcgcg cgttcgtagc cctcggcccc gaagcggcgc cgcgccgccg
    11521 acagtatgtc ggcgcgcgtc tggctcgcgt tgcgaggccg cgtcgttgtc acccagaaac
    11581 tgtatgccac caggcgttaa gttccgccca gcccggaggc taggtgggtg cgggtccgac
    11641 cagtagcccc cgaaacaccg gggccgccca ccggcgcagc tcgtcgcggg tgaggtcggc
    11701 aaccggaagg ttgccgagaa cggaccgggt ggcggcaagc ccgatgacga aggcgtccgt
    11761 gaccgcgatc cgctgcgcgg catggtccgg cgttgccgcc ttcagcaccg gcgcgacgtg
    11821 ggtggcaagc gtttcgttca gggtgtccgc cgcgacccgg cttgtcatcg ccgcctgcag
    11881 cagcgccagg aacgagtggt cgtcctccca gaccgcgaag taccgcggca gcagcatgtc
    11941 cgcgatctcg tccggttcag caccggagag gtccgggagt tcgatggtga attcggtcgc
    12001 cgtcgcgaaa aggtcctgct tgctaccgaa gtagcgaatc accagcgccg cgtccacgcc
    12061 cacgtcggcg gcgatggcac gcagcgtggt tcgttcgaag ccttcggtgg cgaatcggcg
    12121 gcgcgccgcg tccaggatgt cggcgcgcgt ctgggcggcg tttcgaggcc gattaggact
    12181 catccccaaa tgctatgtca gccactgttg acttagccag caaccgtggc gtatgttcta
    12241 agtcaccgag tgttgacaaa agtcaccgag tgttgaaatt acgacgcgga gggcgggtgg
    12301 acgccatggc atgcggcacc gggcggtctg gcactgcgac tcgtgacacc gcgcctcccg
    12361 gcgcgacggc ccctccgatc ggcgatgcgg cccctcgccg tggcgtgctg caccgcatcg
    12421 cggcgctggc catctgcgcg ccgcgccgaa ccctggccct aacggcactc gtgatggcgg
    12481 tcgcgggagt cttcggggcc cccgtggccc agagcctgtc ggcgagcggc ttccaagacc
    12541 ccacttcgga atccgcccgg gccgccacga tcctgaccga caagttcggc cagggtgagg
    12601 tgcaattgct gttcgtggtg tccgcaccgc aagggtttga cagtccggcg gcgcgttcgg
    12661 tcggcaccga catcgccgat cagctgagcc ggtcaccgca cgtgaccagc gtgacctcgg
    12721 cgtggacggc cccgccggcg gcggccacat cgctggtcag caaggatggg ctgtccgggc
    12781 tggtcgtcgc cggcatcgga ggcggcgaga ccccgtcgat catgaaggcg cagaagtacg
    12841 ccgacgagct gtcacagcaa ttcgcacacg accgaaacgg tgtcaccgtg cgctccggcg
    12901 gggtggcgat ggtcaacgcg cagatcaccg cgcaggccca gcacgacgtg ttggtgatgg
    12961 aggcgatagc catcccgctc agcttcgtcg tgctggtctg ggtgttcggc ggcttgttca
    13021 cggcggcgct gccggtcgcc atcggcggga tgtcgatcct ggcctcgctc gcggtcctgc
    13081 ggctgatcgc cttcgccact gacgtgtcga tcttcgcgct caacctcacc accgcgctgg
    13141 gcctggcgct ggcgatcgac tacacgttgc tgatcgtcag ccgcttccga gacgaaatgg
    13201 ccgccggcgc aacccgtgac gacgcactgc tgcgcaccat ggcgaccgcg ggccgcacgg
    13261 tgttgttctc ggcgaccacc gtcgcgctgt cgatggccgc gatgatccag ttcccgatgc
    13321 acttcctgaa gtcgttcggc tacgcgggca ttgccacggt cgcgttcacc gcgatcgcgg
    13381 cggtggtgat gacacccgcg gccatcgtgc tgctcgggga tcggctgaat gcgcttgacg
    13441 tgcgtcggct ggtgcgtcgc ctggccgggc gcccgcggcc ggcgcggaag ccagtcgagg
    13501 acgagttctg gtaccgctca acgaatttcg tcacccgtcg cgcagttccg gtcggggtgg
    13561 cggtggtcgc cgtgctgctg ttgttggggg cgccgttcct cggtgtgcgg tgg
//
LOCUS       DASSGQ010000264         7006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_777829,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000264 DASSGQ010000000
VERSION     DASSGQ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777829"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VFI55_11670"
     CDS             complement(<1..675)
                     /locus_tag="VFI55_11670"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET7074726.1"
                     /translation="MALCTRLTEFFDIEYPILLAPMAMISGGRLAEAVTSAGGLGLIG
                     GGYGDAHWLQREFDRADGTRVGVGFITWSLAREPELLDAALARRPATVMLSFGELRPF
                     VERVHAAGVPLTAQVQNLDHARRALDAGADILVAQGGEAGGHGMTARSTFTLVPDVVD
                     LVAERSPETLVVAAGGVADGRGLAAALALGADGALVGTRFWASEEALVSPRAHQRALQ
                     ASGDDTR"
     gene            complement(684..1076)
                     /locus_tag="VFI55_11675"
     CDS             complement(684..1076)
                     /locus_tag="VFI55_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009955983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HET7074727.1"
                     /translation="MPLLYIDLIEGRTPSEVRALLDAVHDAVVGAFEVPPRDRYQVVH
                     THPAHEIVAWDTGLGITRSSQQVIVHVVSRRRARESKEKFYALLASNLTERCGLDAAD
                     VIVSITENGDEDWSFGHGRAQFLTGELK"
     gene            1188..2063
                     /locus_tag="VFI55_11680"
     CDS             1188..2063
                     /locus_tag="VFI55_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7074728.1"
                     /translation="MGVLDSGRVELRHLRAFEAVARLRSFTHAAAELSITQPALSRTI
                     RQLEDALGVTLLDRGSRHVDTTAAGRIFLDHVERTLAELERGFGALSRQASIRLGFSW
                     LLPDPWAQDTVARFERATGTMVTLIRTDDALGAVQQGKVDLAVVRGRVTSTAVRVVHL
                     FNESRVAVCSVHSPLAARAELDWAEVPRWPLVVNTASGTTGPWSWPAGAGPKTVVETT
                     NFDEWIESVAADRGIGVIPDVAVRRNIHPGVRFIALRGAPDSPVGLAFLPRAHNAVLR
                     RFVEAAVASSPQAGA"
     gene            complement(2078..2938)
                     /locus_tag="VFI55_11685"
     CDS             complement(2078..2938)
                     /locus_tag="VFI55_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007323353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7074729.1"
                     /translation="MTTIQTRAGRVAYRESGTGPTILLLHATLHDGHDFDPIIETLAR
                     RYRTIAVDWPGHGDSDPIDAALDPSAPLLADVLEDVVDGLGLSRAVLIGNSVGGYAAA
                     RLAINRPESVAGLVLVNSGGFVPWNPLSRNLCRVLGTQAVFRRAAPLLIRGYMKAKTD
                     SDRRIIERATAAAQTLEGVRIAAGLWRSFATPGHDLRSRAGELRAPTLLVWGKKDIVI
                     PVSGGRATHDAIPGSRLEILDTGHVVFSSDPGGFLAVAEPFLRSIDSRVCAHGVDTPR
                     DPALGAHRKP"
     gene            3024..3359
                     /locus_tag="VFI55_11690"
     CDS             3024..3359
                     /locus_tag="VFI55_11690"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HET7074730.1"
                     /translation="MPQVPPPEAPFTDKMAYYRTQHTSKGVRATHLVGTPIIAAGLPL
                     MFAKPKVGATMFVGGWAMQIIGHRLFEKNFPSTHKGWITYQLTGVIDVCERYGEALAR
                     RSQRKAGLR"
     gene            3356..3901
                     /locus_tag="VFI55_11695"
     CDS             3356..3901
                     /locus_tag="VFI55_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7074731.1"
                     /translation="MRSPEPGRNALLDAGQRLLGTADLARISVNAIVAEAGMAKGSFY
                     QHWPSRADYVRALHARFHDDLEESITAAMAGVPPGRDRLAAGMNTYLDGCLAEPATKA
                     LLVQSRTEAGLSDLVAARNDSAAARVTPDLVALGWAPSEPIAVLLVAAVAEIALLELA
                     AGERNDELRGGLLRLATRTPE"
     gene            complement(3933..4787)
                     /locus_tag="VFI55_11700"
     CDS             complement(3933..4787)
                     /locus_tag="VFI55_11700"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HET7074732.1"
                     /translation="MPAAPTLDVTKAIQFGQLVNATYSTLPTDLSNAAGKPLSAGGID
                     YTVVTTIYANDLATDMNPSRGDDEVSIGLICQANQTGDAVIAIRGTEGIREWAHDAEF
                     LQVPCPFLAGAGHTEDGFTAMYESLRTGATPNSPTVVQALPKLSFPKPLGSLTICGHS
                     LGGALATLLALDVAANTSFTGPAVYSYGSPRTGDSLFAGTFDQVVKNSYRIANRLDIV
                     PALPPPIDYEHVLNPYELNPVRLLPLPPKILVKSTAACEHSLATYLYMLSLQSGGPVL
                     ALESSCQP"
     gene            complement(4804..5412)
                     /locus_tag="VFI55_11705"
     CDS             complement(4804..5412)
                     /locus_tag="VFI55_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003899077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET7074733.1"
                     /translation="MNSTPASTFQRIATVIGGLTLGVALVGCAGHGDGHVTASLTPKV
                     TTVARTVPAAPADGPLTISSPAFPDGAPIPAQYTCKGADTAPGLTWSAPLGAALVVDD
                     PDAVRGPYIHWIVVGIPPGPGNTADGQTPAGGSSLPNSAGQAGYSGPCPPAGTGVHHY
                     RFTLYQLPATFQLAPGLVGGQAAQAIAQAASGQAQFTGTFAG"
     gene            complement(5503..6507)
                     /locus_tag="VFI55_11710"
     CDS             complement(5503..6507)
                     /locus_tag="VFI55_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7074734.1"
                     /translation="MRAVVITKHGDPSVLQVQQRPDPPPPGPGQLRVAVRAAGVNFAD
                     HLARVGMYPDAPKLPAVVGYEVAGTVEAVGDGVDPHRVGERVLAGTRFGGYAEIVNVA
                     ANDSVVLPDALSFEQGAAVPVNYSTAWAALHGYGSLRAGERVLVHAAAGGVGIAAIQF
                     AKAAGAEVHGTASPGKHGRLAELGVDRAIDYRRDGWWRGLDPYDLVLDALGGTSLRRS
                     YALLRPGGRLVAYGVSNMQHGETRSLRAAAPHAMAMLRGFNLLDQLSDSKAVIGLNML
                     RLWDDRGTLEPWITPLKKALDDATIVPIVHAAVPFADAPRAHRILAARENVGKVVLVP
                     "
     gene            complement(6581..7003)
                     /locus_tag="VFI55_11715"
     CDS             complement(6581..7003)
                     /locus_tag="VFI55_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET7074735.1"
                     /translation="MSETDRAAKTLATIRVDQFVAAPPAKVWRLLTEPELLRLWWAEG
                     EVAAVVGHQFTLDMPGYGKQPCKVLQVDPPHRFVYTFTPAWTLSWSLEPEGAGTRVFL
                     EHSGFDLDDKRMADAFNRMGPGWCEVVLPRLCEAATRT"
BASE COUNT         1118 a         2366 c         2401 g         1121 t
ORIGIN      
        1 acgggtgtcg tctccgctgg cctggagcgc ccgctggtgt gctcgtggcg acaccagcgc
       61 ctcttcggag gcccaaaacc gggtccccac cagggcgccg tcagcgccga gcgccagcgc
      121 cgcggccagc ccgcggccat cggcgacgcc gcccgcggcc accaccagcg tttcgggcga
      181 acgttcggcg acgaggtcga cgacgtcggg caccagcgtg aaggtggagc gcgcagtcat
      241 tccgtgccca cccgcctcgc cgccctgcgc cacgaggatg tctgcgccgg cgtccagcgc
      301 ccggcgcgcg tgatcgaggt tttgcacctg cgcggtcagc ggaacgccgg cggcgtggac
      361 gcgctcgacg aacggtcgca gttcaccgaa ggacaacatc accgtagcgg gtcgccgcgc
      421 cagggccgcg tcgagcagct cgggctcgcg ggccaggctc caggtgatga acccgactcc
      481 gaccctcgtc ccgtcggccc ggtcgaattc gcgctgcagc cagtgcgcgt cgccgtatcc
      541 tccgccgatg agtcccagac cgccggccga tgtcaccgcc tccgccagcc ggccgccgga
      601 aatcatcgcc atgggcgcga gcaggatcgg gtattcgatg tcgaagaatt ccgtgagccg
      661 ggtgcacagt gccatagcct cgctcatttc agttctccag tgaggaactg tgcgcggcca
      721 tggccgaaag accaatcctc atcgccgttt tcggtgatcg agacgatcac atcggccgca
      781 tccagcccac accgctccgt caggttggac gccagcagtg catagaactt ctccttcgat
      841 tcgcgggccc gacgccggct gaccacatgc acaatcacct gctgggacga gcgggtgatg
      901 cccagcccgg tgtcccaggc gacgatctcg tgcgcgggat gggtgtgcac cacctggtag
      961 cggtcacggg gcggaacctc gaatgcgccg accacggcgt cgtgaacggc atccagcaac
     1021 gcccggacct ccgacggtgt ccggccctcg atgaggtcga tgtacaagag cggcattttt
     1081 ctcctcattt ctcctgcccg tgatggcgtg tgtggcctgg ttatgacgtt aggttcgcgg
     1141 aaaccggctg tgaaatgcct gctgtagtcg atctatgctt gccaggcatg ggtgtactgg
     1201 atagcggccg agtcgagttg cgccacctcc gggcgttcga ggcggtggcg cgactccggt
     1261 cgttcaccca cgccgccgcc gaactcagca tcacccagcc cgcgctgagc cgcaccatcc
     1321 ggcagttgga agacgcgctc ggggtgacac tgctggaccg cggttcacgg cacgtggaca
     1381 cgacggcggc cgggcggatt tttctcgacc acgtcgaacg gacgctcgcc gagctggagc
     1441 gggggttcgg cgccctgagt cggcaagcca gcatccgcct cgggttcagt tggttgttgc
     1501 ccgacccctg ggcgcaggac accgtcgctc gtttcgaacg ggccaccggc acgatggtca
     1561 cgttgatccg caccgacgat gcgctgggcg ccgtgcagca gggaaaggtt gacctcgccg
     1621 tggtgcgtgg gcgggtgacc tcgaccgcgg tgcgggtcgt gcacctgttc aacgagtcgc
     1681 gggtggccgt gtgttctgtt cactcgcccc tggcggcaag ggccgagttg gactgggccg
     1741 aggttccacg gtggccgttg gtcgtcaaca ccgccagtgg taccaccggg ccatggtcct
     1801 ggccggccgg tgcgggaccg aaaaccgttg tggagacgac gaatttcgac gagtggatcg
     1861 aatccgtcgc ggcggaccgc ggcatcggcg tgattcccga cgtcgccgtc aggcgcaaca
     1921 tccaccccgg cgtccggttc atcgcgctgc ggggtgcacc cgatagcccc gtcgggctgg
     1981 catttctgcc gcgcgcccac aacgccgtgc tgcgccgctt cgtcgaggcg gccgtggcga
     2041 gttcaccgca agccggcgct tgacgtcgcg tgtgcgctca cggcttccgg tgtgcgccca
     2101 gggcgggatc cctcggcgtg tcgacgccgt gagcgcacac tcgactgtcg atcgagcgca
     2161 agaacggctc ggcgacggcg agaaacccgc ccggatccga cgagaacacc acatgcccgg
     2221 tgtcgaggat ctcgagccgc gaaccgggta tggcgtcgtg ggtcgcacgc ccgccgctca
     2281 ccgggatcac gatgtctttc ttgccccaca cgaggagggt tggcgcgcgc agttccccgg
     2341 cgcggctgcg cagatcgtgc cccggcgtgg cgaaacttcg ccaaagcccc gccgcgatgc
     2401 ggacgccctc gagtgtctgc gccgcagcgg tcgcacgctc gatgattcgc cggtcgctgt
     2461 cggtcttggc cttcatgtag ccacggatga gcaggggagc ggcccggcgg aacaccgcct
     2521 gcgtgcccag cacgcggcaa agattgcggg acagtggatt ccagggcacg aacccgcccg
     2581 agttgaccag gaccagaccc gcgacggact cgggccggtt gatggccagc cgcgccgccg
     2641 cgtagccgcc gaccgagttt ccgatcagca ccgcccgcga caggccaagg ccgtcgacga
     2701 cgtcttcgag cacgtcggcg agcagcggtg cgctgggatc cagagcggcg tcaatgggat
     2761 ccgaatcccc atgcccgggc cagtcgaccg caatggtgcg gtagcgccgc gccagcgtct
     2821 cgatgatcgg gtcgaagtca tgcccgtcgt gcagcgtcgc atgcaacaac aaaatcgttg
     2881 ggccagtgcc ggattcgcga taggcaacgc gccccgcccg ggtttgaatg gtcgtcatgg
     2941 ttgcccctct cggtcattga ccgactggtc ggtcatggcg tacgctacgc ccgtaagtgc
     3001 gcccccgcaa gaagggattc gggatgccac aagtgccgcc gcctgaagcg ccattcactg
     3061 acaagatggc ctactaccgg acgcaacaca cctccaaggg ggtgcgggca acccatctgg
     3121 tcggcacgcc gatcatcgcg gccggcctgc cgctgatgtt cgccaagccc aaagtcggtg
     3181 ccacgatgtt cgttggagga tgggcgatgc agatcatcgg tcaccggctc ttcgaaaaga
     3241 actttccctc gacccacaaa gggtggatca cctaccaact caccggcgtg atcgatgtgt
     3301 gcgagcgata cggggaagcg ctggcccggc gcagccagcg caaggcggga ctgcggtgag
     3361 atcacccgag ccgggccgca atgccctgct ggacgcgggc caacggctgt tggggacggc
     3421 cgatctggcc aggatctccg tcaacgcgat cgtcgccgag gccgggatgg ccaaaggcag
     3481 cttctatcag cactggccca gtcgcgccga ctatgtgcgc gcgttgcacg cccgattcca
     3541 cgacgacttg gaagagtcga tcaccgcagc catggccggc gttccgcccg gacgcgaccg
     3601 gctcgccgcc gggatgaaca cctacctcga cggctgcttg gccgaaccgg ccaccaaggc
     3661 tttgctcgtt cagtcgcgca ccgaggccgg cctgagcgac ttggtcgccg cccgcaacga
     3721 cagtgcggcc gcgcgcgtga cgcccgatct ggtggcgctc ggatgggcgc cgtcggaacc
     3781 tatcgccgtg ctgctcgtcg cggcggtcgc cgagatcgcc ctgctcgagt tggccgccgg
     3841 tgagcgcaac gacgagttgc gcggcgggtt gcttcggctc gccacccgaa cgccggagta
     3901 ggcacaccga cgccggggat cgctccggtt ggtcagggtt gacagctgga ttccagcgca
     3961 agcaccgggc cgccggattg cagggacagc atatacaagt aggtcgccag cgaatgttcg
     4021 caagccgcag tggatttgac caggatcttc gggggcagtg gcagtagacg cacggggttc
     4081 agctcatagg gattgagcac atgctcgtag tcgatgggag gaggcaatgc ggggacgatg
     4141 tcgaggcgat ttgcgatccg gtacgagttc ttgaccacct ggtcaaaggt tccggcgaac
     4201 agcgaatccc cggtgcgtgg actgccgtag gagtagacgg ccgggccggt gaaagaggtg
     4261 ttcgcagcca cgtcgagcgc gagcagggtc gccagggccc ctcccaggct gtgcccgcaa
     4321 atcgtcagtg agccaagtgg tttgggaaac gaaagcttcg gcagcgcttg caccacggtc
     4381 ggcgagttcg gcgtggcccc ggtccgcaac gactcgtaca tggcggtgaa gccgtcttcg
     4441 gtgtggccgg cgccggccag gaaggggcat ggcacctgga ggaactcggc atcgtgggcc
     4501 cactcccgga tgccttcggt gccccggatg gcgatcaccg catcgccggt ttggttcgct
     4561 tggcagatca gtcctatgga aacctcgtca tcgccccggc tcgggttcat gtcggtggcc
     4621 aaatcgttgg catagatcgt cgtgaccacc gtgtagtcga tgccgccggc actcagcggc
     4681 ttaccggccg cgttcgacaa gtcagtgggc agtgtcgagt aggtggcgtt cacgagttgg
     4741 ccgaattgga tggccttggt gacatctaat gtcggcgccg cgggcatcgc tcatccgtcc
     4801 gggtcagccc gcgaacgttc cggtgaattg cgcctgtccc gaggcggcct gcgcgatcgc
     4861 ctgcgccgct tgcccaccca ccaggcccgg agcgagctgg aaggtggccg gaagctggta
     4921 gagggtgaac cggtagtggt gcaccccggt acccgcgggt gggcagggcc cgctgtaccc
     4981 ggcctggccg gccgagttcg gcaggctgct tcctcccgca ggagtctgac catccgcggt
     5041 gttgccggga ccgggcggga tccccaccac gatccagtgg atatacggcc cacggacggc
     5101 gtccggatcg tcgacaacga gtgccgcacc cagcggcgcc gaccaggtca gccccggcgc
     5161 ggtgtcggcc cccttgcagg tgtactgcgc cgggatcggc gcgccgtcgg ggaacgcggg
     5221 gctgctgatc gtcaatggcc cgtccgcggg tgccgcgggg acggtccggg cgacggtcgt
     5281 cactttcggc gtcagggagg ccgtcacgtg gccgtcgccg tgaccagcgc agccaaccaa
     5341 cgcgaccccg agtgtcaggc cgccgatgac cgtcgcgatt cgctgaaacg tactcgccgg
     5401 tgtcgagttc atacaggaca gtctcgctcg cgaagcgggg gttcggaagg gcccgtgccg
     5461 cagtgcggtc cggcggcgac cagcgccgcg ccgtcgatcc gactagggca ccaggacgac
     5521 ctttccgacg ttttcccggg cggccaggat gcggtgggcc cgcggcgcgt cggcgaacgg
     5581 cacggccgcg tggacgatcg ggacgatcgt ggcgtcatcg agcgcctttt tcagcggagt
     5641 gatccagggc tccaacgtgc cgcggtcgtc ccacagccgc agcatgttca agccgatcac
     5701 cgctttggag tcggagagtt ggtcgagcag attgaacccg cgcagcatcg ccatcgcgtg
     5761 cggtgcggcc gcccgcagcg agcgcgtctc gccgtgctgc atgttcgaaa ccccgtaggc
     5821 gacaagtctt ccgcccgggc gcagcagtgc ataggagcgt cgcagcgacg tgccgcccag
     5881 cgcgtcgagc accaggtcat atggatcgag cccgcgccac cagccgtcgc ggcggtagtc
     5941 gatcgcccgg tctacgccca actcggccag cctgccgtgc tttccgggcg acgcggtgcc
     6001 gtgcacctca gccccggcgg ccttcgcgaa ttggatggcc gcgatgccca caccgcccgc
     6061 cgcggcgtgc accagcaccc gctcaccggc gcgcagcgac ccgtacccat gcagcgccgc
     6121 ccacgcggtc gagtagttga cggggaccgc cgcgccctgt tcgaagctca acgcgtcggg
     6181 caggaccacg gagtcattcg cggcgacgtt gacgatttcg gcgtagccac cgaatcgcgt
     6241 gccggccagc acgcgttcgc cgacgcggtg gggatccaca ccgtcgccga cggcctcgac
     6301 cgtcccggcg acctcgtagc cgaccaccgc cggaagcttc ggcgcgtccg gatacatgcc
     6361 gacgcgcgcg aggtggtcgg cgaagttgac ccccgcggca cgtaccgcga cccgcagctg
     6421 accggggccg ggcggcggcg ggtcgggccg ctgctgaacc tgcaagaccg acgggtcgcc
     6481 atgcttggtg atgacgactg cgcgcatcgt gctccccaat gttctgcgtg accgctgcga
     6541 gctgactacc cgacgatggt cgcacacgcc ggcggtcggt tcaggtccgc gtcgccgcct
     6601 cacagagacg ggggagaacg acctcacacc agcctgggcc catccggttg aaggcgtcgg
     6661 ccatccgttt gtcgtccaaa tcaaagccgc tgtgctccaa gaacactcgg gtaccggcgc
     6721 cttccggctc gaggctccag gacagtgtcc aggccggggt gaaggtgtag acgaaacggt
     6781 gcgggggatc gacctggagc accttgcagg gttgcttgcc gtagcccggc atgtccaggg
     6841 tgaactgatg tccgacgacc gcggccacct cgccctcggc ccaccacagt cgcaggagct
     6901 cgggttcggt cagcagccgc cacaccttcg ccggcggtgc ggcgacgaat tggtccaccc
     6961 ggatcgttgc gagcgtttta gcggcccggt ctgtctcgct cattcc
//
LOCUS       DASSGQ010000265         8088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_808765,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000265 DASSGQ010000000
VERSION     DASSGQ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8088
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808765"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VFI55_11720"
     CDS             complement(<1..400)
                     /locus_tag="VFI55_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="required for PDIM synthesis; phthiocerol and
                     phthiodiolone dimycocerosate esters are scaffolds used for
                     virulence-enhancing lipids; proposed to catalyze
                     diesterification of phthiocerol and phthiodolone with
                     mycocerosate; functions in polyketide synthesis; Derived
                     by automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET7074736.1"
                     /translation="MFPGTVIRKLAGSEEVFAHNETFFALGVDMTGPVDISAMSEAFD
                     ALLQAHPIFAGCLAPSADGRHQIVADDLLHPGIWLVGSSGSSTAAMQLDQNQSLLNLR
                     LKVDGERSELTLYAHHALADAHHVFGLLEEL"
     gene            complement(429..1259)
                     /locus_tag="VFI55_11725"
     CDS             complement(429..1259)
                     /locus_tag="VFI55_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7074737.1"
                     /translation="MATMVRERITVGDVARSYPENSPRVLIPQTVVQTKRLLVRWSRD
                     PVTLIETLVLPIMFLLTLNIVLGQLISQTTGHSGLYGTVPMNALAAAINGSSIGAVGL
                     IGERTNGLLRRLWVVPVHRASGVLSRILAEAVRILMTTLVVLGAGLLLGFRFTQGAPA
                     TLVWLAIPLIFGMAFATLITMVALYTAKTFLLEGVTLVHILAVVFSTGFLPVDQYPRW
                     IQPVVAHQPMTYAIEAMRGLSLGGPVRTPLVATLLWSVGIAAVCVAPMLIGYRRASTS
                     "
     gene            complement(1259..2071)
                     /locus_tag="VFI55_11730"
     CDS             complement(1259..2071)
                     /locus_tag="VFI55_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006372818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7074738.1"
                     /translation="MTALHTARAHPSAFTQWWVLTARFIKPTLRNGELALTVASSVVF
                     TAGFYVPLSKVMVNATKFVSSSYAQYIMPLVALQAIVFAAISTAFRAATDSRQGINRR
                     FGTMPIAPLTPVAARIGAAAYRCSVALAVAMACGHVIGFRFHRGTVYIVGFCALVIVI
                     GVLLSFAADLVGTGSRNPEAMMPLLILPPLIFGLLSVGVQPVQQFPQWIQPVVRNQPI
                     SQFVVALRALAGDAAPFAVPVTWQVLAPTLAWLAGMAVILVPASALVLSRRS"
     gene            complement(2068..3087)
                     /locus_tag="VFI55_11735"
     CDS             complement(2068..3087)
                     /locus_tag="VFI55_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7074739.1"
                     /translation="MTNANWNFKRGSDKAVLVEGVRKTFGEVVALHEVSFEVNRGEVI
                     GLLGPNGAGKTTIVDILSTLSRPDRGRASVAGYDCVSEPAGVRRSIMLTGQQVAIDDM
                     LTGHENLVMFGRLFGLNRSAARSRATELIEEFDLVHAADRRVSTYSGGMRRRIDIACG
                     LVVRPQVAFLDEPTTGLDPRSRQSIWDLVASFKTTGIATLLTTQYLEEADELADRIIV
                     IDHGRIIAEGTANELKERTGASFCEIVPRAPKDLPAIAEALGSLLPEKHRAALTPESD
                     RIAMPAPQGTRTLVEAVSRLAAADIEVADLALRRPSLDDVFLALTEDPIESPTARQPV
                     AGAVS"
     gene            complement(3099..7553)
                     /locus_tag="VFI55_11740"
     CDS             complement(3099..7553)
                     /locus_tag="VFI55_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020786538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET7074740.1"
                     /translation="MSTESLLDNAIAVVGMAGRFPGANSVSAFWDNLRRGEESIVNLS
                     DDELRTAGIDEKALTNPKYVRRAPILAGIDEFDADFFGIPPQVAQMLDPQHRLFLQCA
                     WHALEDAGCDPARFDGSIGVYGTSSPSGYLLHNLMSHHEPNAILAQGLNFEQFNLFLQ
                     NDKDFLATRVSHQFDLRGPSIAVQTACSSSLVAVHLACQSLLSGECDMALAGGVSLSV
                     PHHVGYWHSPGSMVSAVGQCRPFDVRADGTVFGSGVAMVALKPMQAAIDAGDRIHAVI
                     RGSAVNNDGSMKMGYAAPNPAAQADVIAEAHAVSGIDASTVSFVETHGTGTPLGDPIE
                     ISGLRKAFAVSQEARPGPCALGSVKSNIGHLEVAAGVASLIKTILCLKHKAIPATLHF
                     TSPNPELHLDDSPFTVPSTFGPWEWDGVRRAGVSSFGVGGTNAHVVLEEAPAVPAPAR
                     STEPQVLVLSARTTAALNESRSALAAALAGPDGPDLADVAFTLTRRRKHKITLAAVVH
                     DSEHAATVLRASEHDNVFVGESAPDSSRHQKPESTCDRVVFLSPGQGAQHVGMAQGLY
                     HTEPVFAEHFDVCAAGFRDEFGIDLPTEVFGGSASTLERIDRAHPALFAVEYALGKLV
                     AAYGVQPGALAGHSIGEYVAATLAGVFDLETAIKVVAMRARLLHASPAGAMVAVALGP
                     DAVAEHLVPGVDIAVVNDPGSCVVAGPDEAVRAFTQRLGEHGILARRVRVSHAAHSSS
                     MDAVVPEFEAFLSGVKLHEPHTPLLSNVTGTWMSAEEATDPARWARQIRATVRFSDEL
                     DAMLTDPNRLRVELGPGGSLTGSAVRQPKWSRRHRAVRLMRHPVQNMDDRDAFLLALG
                     QLWSAGVRVDWSPLTGPSEQAGPRLVSLPGYPFAHQRHWVEPSTTVRNGAAATARALP
                     GGSSNGSNGVVAHPAPGAGPSSTEDTLRRIWSRCLGINSIDRKDNFFELGGDSLIAIS
                     IATSAAYEGFEISPQDLYQHPTLAGLAAAIDAQYEASGLTKPPDDAAHPAVPPTIAHF
                     LERGVREADGWRIPLILRLDPKVSVEDVRAVLTAVTNQHDALRLRLVNRAGTWEQRIV
                     APEQADEFDPVVTRSLPDGVDPASPAARQAVLDVLAELAASREAGAPLAAAYISGPAG
                     GTRYLVLSVHEMVADNASREILAADLFTAFTQRLAGAEIALPPTTAAWREWSLRCASL
                     ATHPAVLDTTDFWLESSRATLRLADPEITSAPHLKDLTRLSSVLTSDQTAHVDDARLR
                     LTISIDEILLAALGRTIAQTVGEGVVAVDLEGAGRSVLRPDVDLRRTIGRFTTLYPVP
                     LRCARDGDATESLALVGDRLKSVPHYGIGYGLLRHVYAPTARLLAAEAPADIHFRYAG
                     VVPELPPLDAPVQFDSDAAMPVRDPAPGLGHAIEIRAYRHSGVLHLDWWYDSRRVHRE
                     RAEALAQRFPMALRVLIQDAIDAIPDEDDFGSEPVELALVDLSTLDAG"
     gene            complement(7550..>8088)
                     /locus_tag="VFI55_11745"
     CDS             complement(7550..>8088)
                     /locus_tag="VFI55_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET7074741.1"
                     /translation="LLAAGRRRTGVARLRPDRALIAFPEIRNLGYFSSVVSELDAAGD
                     GGDWAGPEALTGLGAAEAAHLITERLRARIAAVMGYGDRSAVDPTVPLTELGLDSLMA
                     VRIRNTARTDFGAEPPVALLLQGASLTDLAADLVRQLGLTEQQQTAAAGDALRDRAQQ
                     RAAARQDAAMRRKRGRTV"
BASE COUNT         1372 a         2671 c         2780 g         1265 t
ORIGIN      
        1 agagttcctc gagaaggccg aagacgtggt gggcgtccgc cagcgcgtgg tgcgcgtaca
       61 gggtcagctc cgagcgttcg ccgtcgactt tcaaccgcag gttcagcagc gactgattct
      121 ggtcgagttg cattgccgca gttgacgaac ctgaggaccc gaccagccag atgccgggat
      181 ggagcaggtc gtcggcgacg atttggtggc gaccgtcggc ggacggcgcg aggcagccgg
      241 cgaagatcgg atgcgcctgc aggagcgcgt cgaaggcttc cgacatcgcg ctgatgtcga
      301 ccgggccggt catgtcgacg cccagcgcga aaaacgtttc gttgtgcgcg aacacttcct
      361 cgctgcccgc caacttgcgg atcaccgttc cgggaaacac ctgaaaatcc ccttgcttcg
      421 aggctcgctc agcttgtgct ggctcgtcgg tagccgatca gcatcggcgc cacgcatacg
      481 gcggcgatgc ctacggacca caacagcgtc gcgaccaggg gtgtgcgcac gggaccgccg
      541 agcgacaaac cgcgcatggc ctcgatcgcg taggtcatcg gctgatgggc gaccaccggc
      601 tggatccacc gcgggtactg gtccaccggc aggaagccgg tggagaagac gaccgcaagt
      661 atgtgcacca gcgtgactcc ctcgaggagg aaggtcttcg cggtgtacag cgcgaccatg
      721 gtgatcagcg tcgcgaacgc catcccgaaa atcagcggga tcgccagcca caccagtgtc
      781 gccggagcgc cctgcgtgaa acggaatccg agcagcagac ccgcgccgag caccaccagg
      841 gtggtcatca ggatccggac cgcctcggcg aggatccgcg acagcacacc ggacgcgcgg
      901 tgtacgggta ccacccacag ccggcgcagc aagccgttgg tgcgctcccc gatgaggccg
      961 accgcaccga tggacgatcc gttgatcgcg gcggcgagcg cgttcatcgg gacggtgccg
     1021 tagagaccgc tgtgcccggt ggtttgtgaa atcagctgcc cgagcacaat gttcagcgtc
     1081 agcaggaaca tgatgggcag caccagcgtc tcgatcagcg tcacgggatc gcgcgaccac
     1141 cgaacgagga gccgcttggt ctgcaccacg gtctgcggga tcagcacccg cggggaattc
     1201 tccggatagg accgcgcgac atcgccgacg gtgatccgct cccgaaccat ggtcgccatc
     1261 aggatcgcct cgacaagacc agcgccgatg cgggcaccag gatcaccgcc atgccggcca
     1321 gccacgccag cgtcggcgcc aggacctgcc acgtcaccgg caccgcgaac ggtgcggcgt
     1381 cgccggccag cgcgcgcagc gcaaccacga actgggaaat cggctggttg cggacgacgg
     1441 gctgaatcca ctgcggaaac tgttgcaccg gctggactcc gacggagagc agaccgaaga
     1501 tcagtggggg caagatcagc agcggcatca tcgcttcggg gtttcggctg ccggtgccga
     1561 ccaggtccgc ggcgaatgac agcagcacgc cgatcacgat caccaacgcg cagaacccga
     1621 cgatataaac cgtgccacgg tggaaccgga agccgatcac atggccgcac gccattgcca
     1681 cggccaaagc cacggaacac cggtacgccg cggcgccgat gcgggcggcc accggggtca
     1741 atggggcgat cggcatggtc ccgaaccggc gattgatgcc ctgcctggag tcggtggcgg
     1801 cccgaaaggc agtcgaaatg gccgcgaaca cgatcgcctg cagggccacc agcggcatga
     1861 tgtactgcgc gtagctgctg ctgacgaact tcgtggcgtt gaccatgacc ttgctcaacg
     1921 ggacgtagaa gccggcggtg aaaaccacgg aagacgcgac ggtgagagcc agttcgccgt
     1981 tgcgcagggt gggcttgatg aagcgtgcgg tgagcaccca ccactgcgtg aacgccgacg
     2041 ggtgtgcccg cgcggtgtgc aaggcggtca cgaaacggcc cctgcgactg gctgtctcgc
     2101 ggtgggcgac tcgatggggt cctccgtcaa ggcgaggaac acgtcatcca gcgacgggcg
     2161 ccgcagcgcg aggtcggcca cctcgatgtc ggccgccgcg agccgcgaga cggcctcgac
     2221 cagtgtgcgg gtgccctgtg gtgccggcat cgcgatccgg tccgattcgg gtgtcagcgc
     2281 ggcacggtgc ttctcgggca acagcgagcc gagcgcctcg gcgatggccg gcaggtcctt
     2341 cggggcgcgt ggcacgatct cgcagaaact cgcaccggtg cgctccttga gctcgttggc
     2401 ggttccctcc gcgatgattc tgccgtggtc gatcacgatg atccgatcgg cgagctcgtc
     2461 ggcctcttcc aggtactgcg tggtcagcag tgtggcgatg ccggtggtct tgaagctggc
     2521 gaccaggtcc caaatgcttt gcctgctcct gggatccagc ccggtggtgg gctcgtccaa
     2581 aaacgccacc tggggccgga ccaccagacc gcaggcgatg tcgatgcgcc tgcgcatgcc
     2641 gcccgagtaa gtgctcaccc gccgatcggc ggcatgcacc agatcgaact cttcgatgag
     2701 ttcggtcgcc cgggagcgcg ccgccgacct gttcagtccg aacagccggc cgaacatcac
     2761 caggttctcg tgaccggtca gcatgtcgtc gatcgccacc tgctgacccg tcagcatgat
     2821 cgaccgtcgc accccggcgg gctcggaaac gcagtcatag ccggcgaccg acgcacggcc
     2881 gcgatcgggc cggctcagcg tcgacaagat gtcgacgatc gtggtcttgc cggccccgtt
     2941 gggaccgagc aggccgatca cctcaccacg attgacctcg aagctgacct cgtgcagggc
     3001 caccacctcg ccgaaggtct tgcgcacccc ttcgacgagg acggccttat cggagcccct
     3061 tttaaagttc caattcgcgt tagtcattca cgtctccctt aaccggcgtc cagcgtcgac
     3121 aagtccacca gagccagctc gacgggttcc gagccgaagt cgtcttcgtc ggggatagcg
     3181 tcgatggcgt cctggatcag cacccgcagc gccatcggga agcgttgcgc cagcgcctcg
     3241 gccctttcgc ggtgaacgcg gcgggagtca taccaccaat cgaggtgcag cacgccggag
     3301 tggcggtacg cccgaatttc gatggcatga cccagccctg gggcggggtc acgcaccggc
     3361 atcgccgcgt cggagtcgaa ttgaaccggc gcgtcgagcg gcgggagctc gggaaccact
     3421 ccggcataac ggaagtggat gtccgcgggg gcttcggccg cgagcagccg cgcggtgggc
     3481 gcatagacgt ggcgcaacag gccgtacccg atcccgtaat gcggaacgga tttcaggcgg
     3541 tcgcccacca aggccagcga ctcggtcgcg tcaccgtccc tggcgcaccg gagcggcacc
     3601 gggtagagcg tggtgaagcg gccgatggtg cggcgcaagt cgacgtccgg ccgcaggacc
     3661 gaacggccgg ctccttccag gtctaccgcg accacacctt cgccgacggt ctgggcgatc
     3721 gttcgaccca gcgcggccag caggatttcg tcgatcgaga tggtcagcct gaggcgggcg
     3781 tcgtcgacgt gggctgtctg gtcgctggtc agcaccgacg acagtcgcgt caggtctttc
     3841 agatggggtg ccgacgtgat ttcgggatcg gccaaacgca gcgtcgcccg tgagctctcc
     3901 agccagaagt cggtggtgtc caggaccgcc gggtgcgttg ccagggaagc gcatcgcagc
     3961 gaccactcgc gccaggcagc cgtggtcggt ggcagcgcga tttcggcacc ggccagccgt
     4021 tgcgtgaacg cggtgaagag atcggccgcc aggatttccc gcgacgcatt gtcggcgacc
     4081 atctcgtgca cggataggac caggtagcgc gtgccgcctg ccggaccgga aatgtaggcg
     4141 gcagccagtg gggcgccggc ttcccggctg gcggcgagct cggccaggac gtccaaaacc
     4201 gcttggcgcg cagcgggact cgctggatcc accccgtcgg gcagtgaccg ggtcacgacc
     4261 gggtcgaatt cgtcggcctg ctcaggcgcc acgatgcgtt gttcccaggt cccggcgcgg
     4321 tttaccagcc gcagccgcaa cgcgtcgtgc tggttggtca ccgcggtcag cacggcgcgg
     4381 acgtcctcga cactcacttt cgggtcgagg cgcaggatca acgggatccg ccagccgtct
     4441 gcttcccgca caccgcgctc gaggaagtgc gcaatggtcg gcggaaccgc ggggtgggcc
     4501 gcgtcgtccg gcggcttcgt cagcccgctc gcctcgtatt gagcgtcgat ggcggcggcc
     4561 agcccggcca gcgtcggatg ttggtacaga tcctgcggac tgatctcgaa tccttcgtag
     4621 gcggcgctgg ttgcgatgct gatcgcgatc agcgagtcgc cgcccagttc gaagaaattg
     4681 tctttgcggt caatggagtt gatgccgagg caccgcgacc agatgcggcg caaggtgtcc
     4741 tcggtgctgg acggtcccgc accgggagcc ggatgggcga cgacaccatt ggagccgttg
     4801 ctggagccgc ccggcagtgc ccgcgcggta gcggccgcgc cgttccggac ggtggtgctg
     4861 ggctcgaccc aatgccgttg gtgtgcaaag ggataaccgg gcaggctgac cagccgtggc
     4921 cctgcctgtt cggacggccc ggtcagcggt gaccagtcga cccgcacgcc ggccgaccag
     4981 agttggccaa gagcgagcag gaacgcgtcg cgatcgtcca tgttttgcac ggggtggcgc
     5041 atgagccgga cggcgcggtg cctcctcgac cacttcggct gtcgcaccgc cgaacccgtc
     5101 agactgccgc cgggaccgag ctcgacccgg aggcggttgg gatcggtcag catcgcgtcg
     5161 agttcgtccg agaacctgac cgtcgcgcgg atttggcgcg cccacctcgc cgggtcggtc
     5221 gcctcctcgg cggacatcca ggttccggtg acattgctca gcaggggtgt gtggggttca
     5281 tggagtttca cgccggaaag gaaggcttcg aattccggca ccacggcgtc catcgagctg
     5341 gagtgggccg cgtgcgacac gcggacccgt cgcgcgagta tcccgtgctc acccagccgc
     5401 tgggtgaatg cccggaccgc ctcgtcgggc ccggcgacca cacagctgcc cggatcgttg
     5461 accacggcga tgtcgacgcc gggcaccaaa tgctcggcga cggcatcggg tcccagcgcc
     5521 accgcgacca tggcgccggc cggcgacgcg tgcaggaggc gggcgcgcat cgcgaccacc
     5581 ttgatcgcgg tctcgagatc gaataccccg gccagggtgg ccgcgacgta ttcgccgatg
     5641 ctgtgtccgg ccaacgcccc cggttgcaca ccgtaggccg cgaccaattt gcccagcgcg
     5701 tattcgacag cgaacagcgc gggatgggcg cggtcgatgc gttccaacgt gcttgcggac
     5761 ccgccgaaca cctcggtggg caggtcgatc ccgaattcat cgcggaatcc cgcggcgcag
     5821 acgtcgaagt gctcggcgaa aacgggctcg gtgtgataga ggccctgggc cattccgaca
     5881 tgctgggcgc cctgcccggg tgacaggaaa acgacacggt cgcaagtcga ttcaggtttt
     5941 tgatggcgtg acgaatcggg ggcggattcg ccgacgaaga cattgtcgtg ctcggacgcc
     6001 cgaagcaccg tggccgcgtg ttcggagtcg tgaacgacgg cggccagggt gatcttgtgc
     6061 ttgcggcgcc gggtgagggt gaacgcgacg tccgccaggt ccgggccgtc cgggccggcc
     6121 aatgcggcgg ccagcgccga ccgcgactcg ttgagcgccg ccgtcgtgcg ggccgacagc
     6181 accagcacct ggggttcggt cgacctggcg ggtgccggga ccgccggtgc ctcctcgagc
     6241 accacgtgcg cgttggtccc gcccaccccg aacgagctca ccccggcccg gcgcactccg
     6301 tcccactccc acgggccgaa cgtgctgggc accgtgaacg gactgtcgtc gagatgcagt
     6361 tccgggttcg gactggtgaa gtgcagggtg gccggaatgg ctttgtgctt caggcacaga
     6421 atcgttttga tcaggctcgc gacgccggcg gccacttcga gatggccgat gttcgatttc
     6481 accgacccga gcgcacacgg gcccggtcgg gcctcctgag ataccgcgaa agcctttcgg
     6541 aggccgctga tttctatggg gtcgccaagg ggcgtgccgg ttccgtgggt ctccacaaag
     6601 ctcacggtcg aggcatcgat gcccgacacc gcatgggctt cggcgatcac atcggcctgc
     6661 gcggccggat tgggcgccgc ataacccatt ttcatggatc cgtcgttgtt gaccgccgac
     6721 ccgcggatga cggcgtgaat ccggtcaccg gcgtcgatgg cggcctgcat cggtttgagc
     6781 gccaccatcg cgacaccgct gccgaacacc gtgccatcgg cccgcacatc gaacggccgg
     6841 cactggccga ccgccgacac catcgatccc ggcgaatgcc agtagcccac atggtgcggg
     6901 acgctcagtg agaccccgcc ggcaagagcc atgtcacatt cacccgacag caggctctgg
     6961 caggcgaggt gcacggcgac cagcgacgac gagcacgcgg tttggaccgc gatgctcggg
     7021 ccacggaggt cgaactgatg agacacccgc gttgcgagaa agtccttgtc gttctgcagg
     7081 aataggttga actgctcgaa gttgagtccc tgcgccagga tggcgttggg ttcgtggtgc
     7141 gacatcaggt tgtgcagcag atagccgctc ggggagctgg ttccgtacac gccgattgag
     7201 ccgtcgaacc gcgcggggtc gcagccggcg tcctccagcg catgccatgc gcactgcagg
     7261 aacagccggt gctgcggatc cagcatctgg gcgacttgcg gtgggattcc aaagaagtcg
     7321 gcgtcaaact cgtcgatccc ggccagtatc ggcgcgcgcc gcacgtactt cgggttggtc
     7381 agcgccttct cgtcgatccc ggcggtcctg agctcgtcgt ccgacaggtt gacgatcgac
     7441 tcctcgccgc gccgaagatt gtcccagaac gccgacacag agttggcgcc cgggaatctg
     7501 ccggccatgc cgacaaccgc gatggcgttg tcgagcaacg actccgtgct cataccgttc
     7561 ttcctcgttt ccgccgcatc gcggcatcct gacgcgccgc cgcgcgctgt tgagctctgt
     7621 cgcggagagc gtcaccggct gccgcggtct gctgctgttc ggtgaggccg agctggcgga
     7681 ccaggtcagc ggccagatcg gtcagcgagg cgccctgcaa tagcagtgcc accggcggct
     7741 cggcaccgaa gtccgtacgg gcggtgttgc ggatccgcac cgccatcaaa gaatccagcc
     7801 ccagctcggt cagcggcacg gtcggatcga cggccgagcg atcgccgtag cccatgactg
     7861 ccgcgatccg ggcgcgcagc cgttccgtga tgaggtgcgc ggcctcggcg gcgccgagcc
     7921 cggtgagcgc ctcgggaccg gcccaatcgc cgccatcgcc ggcggcgtcg agctcgctga
     7981 ccacgctgct gaagtagccg aggttgcgaa tctcgggaaa ggcgatcagt gcccggtccg
     8041 gacgcagccg ggcgacgccg gtacggcgac ggccggcggc cagcagcg
//
LOCUS       DASSGQ010000266         5755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1416641,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000266 DASSGQ010000000
VERSION     DASSGQ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416641"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /gene="ligC"
                     /locus_tag="VFI55_11750"
     CDS             complement(<1..317)
                     /gene="ligC"
                     /locus_tag="VFI55_11750"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218248.1"
                     /note="catalyzes the ATP-dependent formation of a
                     phosphodiester at the site of a single-strand break in
                     duplex DNA; in mycobacteria LigC has weak intrinsic nick
                     joining activities and is not essential for growth;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET7074742.1"
                     /translation="MDLPVMPPISPMLAKSVKTIPPDASYEPKWDGFRSICFRDGDQV
                     ELGSRNEKPMTRYFPELVAAVTAELPERCVVDGEIVVATDHGLDFEALQQRIHPADSR
                     VRML"
     gene            355..1395
                     /gene="ligD"
                     /locus_tag="VFI55_11755"
     CDS             355..1395
                     /gene="ligD"
                     /locus_tag="VFI55_11755"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET7074743.1"
                     /translation="MAAAAEELDVDGIAVRVTNPDKVYFPALGSNGTKRRLIEYYLAV
                     ASGPMLTALRDRPTHLQRFPDGIDGEEIYQKRVPQHHPDYLETCRVTFPSGRTADALK
                     VTHPAAIVWAAQMGTITLHPWQVRCPDTEHPDELRIDLDPQPGTGFDEARAVAVNVLR
                     PLLDELGLAGYPKTSGGRGIHVFLRIATDWDFVEVRRAGIALAREVERRAPDAVTTSW
                     WKEERGERIFIDFNQNARDRTMASPYSVRRTPIATVSTPLTWDELATADPDDYTMATV
                     PDLLQRRDDPWAGMEDVVQSIAPLLEMAEADEERGLGDMPYPPNYPKMPGEPKRVQPS
                     RDTDLTRKNKSK"
     gene            1595..1945
                     /locus_tag="VFI55_11760"
     CDS             1595..1945
                     /locus_tag="VFI55_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074744.1"
                     /translation="MAVQTMDWNWAWVFGGDHGYTLSHQYNFAPANCMAQTSLSVGSP
                     GLGIIGFTEYVTRPHRDGADHIHHLSLVTADGGAICYPPVVYDQHLTGVTWVLECGGG
                     LDTMAGSILMFTFG"
     gene            1959..2243
                     /locus_tag="VFI55_11765"
     CDS             1959..2243
                     /locus_tag="VFI55_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074745.1"
                     /translation="MTDASPNRPIHSTKACVVYDAATGQIHHLHRVVTFVGGREPSAD
                     EIEADALRAVSKLRRPHQGDLHALHLDTDTLQPNRKYRVDPATKVLVTDS"
     gene            2547..3896
                     /locus_tag="VFI55_11770"
                     /pseudo
     CDS             2547..3896
                     /locus_tag="VFI55_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
     gene            4229..4846
                     /locus_tag="VFI55_11775"
     CDS             4229..4846
                     /locus_tag="VFI55_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074746.1"
                     /translation="MERRNFRTVRVIRTSNEPVRLRRNEVAFVEIPRPWAAAVASLIS
                     LSAEYRDEGLLVTNVGPASGAAASGIARGDVLLRCDGVSLDSVESLRRLTSRSPRSGG
                     AKELTVEALRGADEMTFQVAAGPLGITVSPLLHRLGPLRRTGLRQTPVAPDDEPPPFD
                     ETTLVEVPAELLPKVRFIKRAIEEPVTARQRKRAEALLTTAARLA"
     gene            complement(4862..5254)
                     /locus_tag="VFI55_11780"
     CDS             complement(4862..5254)
                     /locus_tag="VFI55_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074747.1"
                     /translation="MPEVTINVPGVMTGASFTKWGITFYEWSFLVTIDGQVWVYTDWA
                     DTNNPADEPGLEQNVIEQLENGQGDDNLGGIAYNDSPGDQGQYGDYGGEYGGDYGDYG
                     DFGDYGDFGDDGALADGGGGDDGGGESA"
BASE COUNT          966 a         1830 c         1993 g          966 t
ORIGIN      
        1 agcatccgca cccgcgaatc ggccggatgg atgcgctgtt gcagcgcctc gaaatccagg
       61 ccgtggtcgg tggcgacgac gatctcgccg tcgaccacgc atcgttctgg cagctctgcc
      121 gtcaccgccg cgacaagctc ggggaagtag cgggtcatcg gcttttcgtt acggctgccc
      181 agctcgacct gatcgccgtc gcggaagcag atggaccgga agccatccca tttgggttca
      241 taggacgcat ccggtggaat cgtcttgact gacttggcca gcatcggcga gatcggcggc
      301 atgacgggta agtccattgg ttcattctgg cctccggcgt gtaggaatcc agatatggct
      361 gctgcagcag aagaacttga cgtggacggc atcgcggtgc gggtgaccaa tcccgacaag
      421 gtgtacttcc ccgccctcgg gtcgaacggc acgaagcggc ggctcatcga gtactacctc
      481 gcagtggcaa gcggtccgat gctgactgcg ctgcgtgacc ggcccactca tctgcagcgc
      541 ttccccgacg gcatcgacgg cgaggagatc taccagaagc gggtaccgca gcaccatccc
      601 gactacctgg agacctgccg ggtgacattc ccgtcgggcc ggaccgccga cgcgttgaag
      661 gtgacccatc ccgcggcgat cgtgtgggcg gcgcagatgg gcacgatcac gctgcacccg
      721 tggcaggtgc gttgccccga caccgagcac cccgacgaat tgcgcatcga cctggacccg
      781 caaccgggca ccggtttcga tgaggcgcgg gcggtcgctg tcaacgtgtt gcgaccgctg
      841 ctcgacgaac tcggtctggc cgggtacccg aagacctcgg gaggtcgcgg gatccatgtg
      901 ttcctgcgga tcgccaccga ctgggacttc gtcgaggtgc gccgggcggg tatcgcgctg
      961 gcccgcgagg tggagcggcg cgcgccggac gcggtgacca cctcgtggtg gaaagaggag
     1021 cgcggcgagc ggatcttcat cgacttcaac cagaacgccc gggaccgcac catggcgtcg
     1081 ccgtattcgg tgcggcgcac cccgatcgca accgtgtcga cgccgctgac ctgggacgag
     1141 ttggccaccg cggatccgga cgactacacc atggcgaccg tgcccgatct gctgcagcgt
     1201 cgagatgacc cgtgggccgg tatggaggat gtcgtgcagt cgattgcgcc gttgctggag
     1261 atggccgagg ccgacgaaga gcggggactc ggcgacatgc cgtatccgcc gaattacccg
     1321 aagatgccgg gcgagccgaa gcgggttcag ccgagccggg atacggacct gaccaggaaa
     1381 aacaaatcaa agtgacgcgg tggaccatcg atgccaaagc ggagctaagg cctggtggtt
     1441 taccggcgat aggcgtgaaa gaaccccatg caccacgccc gtggacttga cccgcccgta
     1501 cgcccaagtg ctagaaggtg tttgcctgcg agaagcgatt tcggtgctag cgtacgagac
     1561 gcggtttcac agacccaggg acgaggcggt cgttatggca gttcaaacca tggactggaa
     1621 ttgggcgtgg gttttcggag gggatcacgg ctatacgctg agccaccaat acaacttcgc
     1681 ccccgcaaac tgcatggctc agacgtcgct ttcggtagga agcccgggcc ttggcatcat
     1741 tgggttcacc gaatatgtga ccaggcccca tcgcgatggc gcggaccaca tccaccacct
     1801 atcgcttgtg acggccgatg gcggcgccat ctgttacccc ccggtggttt acgaccagca
     1861 tctgacaggg gtgacctggg tgctggagtg tggtggcgga cttgacacga tggctgggtc
     1921 gatattgatg ttcaccttcg gctgagagag cttcacccat gactgacgcc agtccgaacc
     1981 gcccgatcca ttcgaccaag gcctgcgtcg tctatgacgc cgccaccggg cagattcacc
     2041 accttcaccg cgtggtcacc ttcgtcggtg gccgtgagcc ttcggcggac gagatcgagg
     2101 ccgacgccct ccgtgcagtg agcaagcttc gtcggccgca ccagggcgac ctgcatgcct
     2161 tgcatctaga taccgacacc ctgcaaccga accggaaata tcgcgtggac cccgccacaa
     2221 aggtcctcgt taccgactct tgataagagt ccaaggaggt ttacgaggta ggcccggaag
     2281 cgatcgcgcc gatcatcgtc tcgtcgctgg ccagtaccgc cgtgtgatga cgatcagccc
     2341 gggaccgtat ctgtgcgtcc cggcgccgcg ccgcgactgc ggcggccacc gcgggatcct
     2401 gttcggcacc aacgggacac cggggccccc tagccatacc tataggcagc ccccgagaag
     2461 agtgggggcg gtcctccctt gtcgacgtca caggtttcgc gggatacttt ttgctatccc
     2521 gggggcgatg actcgggagg atagaaatgc cgagtgtgat cgcggcgccg gagctgatcg
     2581 ctacggcggc aacggatttg ggaaatatcg gttcgacgct gagcgcggcg cgcacggccg
     2641 ctgcggcctc gaccgtcacg atgctgcccg cggctgagga tgaggtttcg gcggccatag
     2701 cgtcggcgtt ctcggcccac ggtcagagct ttcaggcact cggcgcgcaa gtggcgtcgt
     2761 ttcatgacca gtttgtgcag gcgttgaccg cgggtgcggg atcctatgcc aacgcggagg
     2821 ccgccaacgt cgcggcgttg acggccaatc ctgcacagac gatagagcag gatctgctca
     2881 gtgtggtgaa tgcccccttc ttggcgttat tggggcgtcc gctggtcggc aacggcgcca
     2941 acagggcccc ggggacgggg gccaacggcg gggccggcgg catcttgtac ggcgacggcg
     3001 gggccggtgg gtctggcgcg ttcggtcaga acggcggtac cggcgggcct gccggactga
     3061 tcggcaacgg cggccccggc ggagccggct caggcggcgt ggtgtccggc acagccggcg
     3121 gtaacggcgg ggccggcgga ctgctgttcg gccacggcgg tgtggggggc accggcggga
     3181 accaaggggc gggcaatggc gggatgggcg gcgccggcgg ggctgccggc ctgttcggca
     3241 ccggcggcat tggcggcacc ggcgggctca cccagctcgt cggcggccac ggcgggatgg
     3301 gtggcgctgg tgggtccggc ggagtgttcg gcaccggcgg ccttggcggc accggagggt
     3361 tcgcccttgc tggcaccggc ggggccggcg gcagcggggg caacggcggc ctcgtcttcg
     3421 gttccggcgg catcgggggc agtggcggca ccggagctgc cgtcgtcgct tcggtcggtg
     3481 acggcggcat cggcggcaac gggggcaacg gcggtctcct cttcggttcc ggcggcgccg
     3541 gcgggagggg tggcaccggc ggcggcagcc ccgtgatggg cggggccggc ggggctggcg
     3601 gggccggcgg ggccgccgcc atgctggccg gcagcggtgg cgcaggcggc atcggcggct
     3661 tcggcctcgg cgccgggggc aatggcgggg gcggcggtaa cggcggcctc ctctacggtt
     3721 ccggcggtac cggcgggagc ggcggcaact ccttgaatcc cggcggcaac ggcggggccg
     3781 gcggcaacgc cgtcctgacg ggtaatggcg gcaacggcgg taacggtggg tccggcacga
     3841 cgacgggcac cgggggccag ggcggcaccg gcgggctgct gatcggcctg aacgggatga
     3901 acgggttgtc gtagccggac ggcagggccg tcccaattgc tgtatgcctg ccgggggtgg
     3961 gggccgaaac gcgttcagcc cggccgggat acggattgca agcgaaagcc gacatgacgc
     4021 cgattagcat catgcaacgc gtcgtcactg atctagcccg tcgttgacaa tttgccagct
     4081 cagtcactac ttttggggac gaggcgccgc gtcgtctcgt tgttaggtta ggcgggttag
     4141 ttcgcgagtt tcgactctgc ttcttgctcg ccttcccttt tttccgggtt ctttccgacc
     4201 ggcggaatca atggtggagg tggctccggt ggaaaggcga aatttccgca ccgtgcgagt
     4261 gatccgcact agcaacgagc cggttcggtt gcgccgcaac gaagttgcgt tcgtcgaaat
     4321 accgcggccg tgggccgccg cggtggcctc gctgatctct ctgtccgcgg agtatcgaga
     4381 cgaaggtttg cttgtcacca atgtcgggcc ggcgagcggc gcggctgcgt ccggaatcgc
     4441 acgtggcgac gtgctgctgc gttgtgacgg cgtttcgctc gacagtgtgg agtcgttgag
     4501 acgactgacc agccgcagcc cgcggagcgg cggggccaaa gagctgaccg tggaggcact
     4561 tcgcggcgcc gacgagatga cattccaggt ggccgcgggt ccgctcggca tcacggtgag
     4621 tccgttgttg catcgactcg ggccgctacg ccgcaccggg ctccgccaga ccccagtggc
     4681 ccccgacgat gagcccccgc cgttcgatga gaccaccctg gtcgaagtgc ctgccgaact
     4741 gctgccgaaa gtgcgcttca tcaaaagggc catcgaggag ccggtaaccg cacggcaacg
     4801 caaaagggcc gaggcgctac tcaccacggc ggcccgattg gcctgacgag atggcggcgc
     4861 gtcacgccga ttcgccgcct ccgtcgtccc cgccaccgcc gtcggccagt gcaccgtcgt
     4921 cgccgaagtc tccgtagtcc ccgaaatccc catagtcccc gtagtcgccc ccatactcgc
     4981 cgccgtagtc cccgtactgt ccttgatcgc ccggcgaatc gttgtacgct atgccgccaa
     5041 ggttgtcgtc gccttgaccg ttttcgagtt gctcgatgac gttttgctcg aggccgggtt
     5101 cgtcagcggg attgttggtg tcggcccaat cggtgtagac ccagacctgg ccgtcgatcg
     5161 tcacgaggaa actccactca taaaacgtga tgccccattt cgtgaacgag gccccggtca
     5221 tcactccggg cacgttgatg gtgacctcag gcatgcgatc ctcctgtctc ggtatggtcc
     5281 gtcggcgcgc gatacgctaa atgccttgtg ccgctgtcga tctcaacagc cggccgcagg
     5341 tagcgacact gcgcgccggc gaaatcgccc aacggcaatt tggcgtaata gccggtgtcg
     5401 caggctcgcc gcacgaacgc cagagccaca tcaccgaaag gcctccacat ccgcagacgg
     5461 ccccgcctgg ccaaacccat caaccgcggc gagagaagct tgaccgtcgt gatcaacgcc
     5521 cgcggcggga gcggaaccac ctcccgcgtc gggccatagc tgcgaaagca cgggtctatc
     5581 acgcaagaat cccgcgtttc ggacaagctg tattcccggc aatgccacag cgcatcggca
     5641 accaactgct cgtcggggac agatcggttc cccggcgaca gcaccagatc gacatccgaa
     5701 ccctggcacc cggattcaag ccgagacccc accagccaca gccggaaggg tcgta
//
LOCUS       DASSGQ010000267         8241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_140562,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000267 DASSGQ010000000
VERSION     DASSGQ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8241
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140562"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VFI55_11785"
     CDS             complement(<1..805)
                     /locus_tag="VFI55_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_178024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPE domain-containing protein"
                     /protein_id="HET7074748.1"
                     /translation="MPDPGWAARPPEANDLLLKTGTGVATMLANQTAWIAVGASHHAS
                     ASASMINTVATSASWAGAGSAGSAANATMLNAALHGLAGWVDVKPAVVSAAISAYETA
                     SAGMRPAPECNANRDESAADNIANPSVLWTLTPRIVALDTEYFGVMWPNNSAVGASYG
                     GILAGLAESLAIPPPLAAMGASPAAPAEAASAIGQAAGDAGAGDGMRAAFSATNGAGA
                     PTSAGQDLGGQLNTFMEPVQSMMGAVPQALQAPSQLMQAPMSAMQPLQSM"
     gene            complement(815..1114)
                     /locus_tag="VFI55_11790"
     CDS             complement(815..1114)
                     /locus_tag="VFI55_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE domain-containing protein"
                     /protein_id="HET7074749.1"
                     /translation="MSWTVQPEAVLGSAGAESAISGEISAAAAAAAPALLGTTPMGND
                     LDSALFTTALNACGGAYVGVVQEHSLQRGLFAGTQSVASSVYTATEAARSLTLGL"
     gene            complement(1449..2066)
                     /locus_tag="VFI55_11795"
     CDS             complement(1449..2066)
                     /locus_tag="VFI55_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005569496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7074750.1"
                     /translation="MLRDPPPTARGVRTRAALIEGARRVFQRSGYLDAQITDITKAAR
                     CSTGTFYTYFSNKDEILQAVLEQVQDDMLHPGIARLDDDSEMSPYALIEASNRAYLRA
                     YQRNAKLMMLLEQVAIADPNFRETRRRRGQAFVVRNAHVIADLQKRGLADPRLDAYQA
                     SRALSAMMSRVAYFSFCLGDDTPIEVLVETSTRIWANALKLPTPT"
     gene            complement(2195..3235)
                     /locus_tag="VFI55_11800"
     CDS             complement(2195..3235)
                     /locus_tag="VFI55_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005265325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET7074751.1"
                     /translation="MSDAAPSDTVAAESRLDLVAEWIATFDVLSPPLSARRIGHGQSN
                     LTYRLTDTDGRQWVLRRPPRGHVLQSAHDIGREHRIIAALQGSEVPVPRVFGMWTVDG
                     VPNLLMEYVDGLVIEDPASAQSLTTAARRSVGASMARALAAVHAVELADVQLLDLASH
                     KPYAPRQLSRWARQLDASRTRALPDLDRLTDLLTQRLPESNGLCLVHGDLHTRNIICD
                     PATGNVRAALDWELSTLGDPLADLGTLLAYWPHNGETDCADVFAPSTLPGFAAREEII
                     ATYADTTGRDVSALGFWHVLGLWKLAVIVEGIRKRISDEPANAAPSGAPPAAYTEDLL
                     RRAWALVGEYGL"
     gene            complement(3232..3357)
                     /locus_tag="VFI55_11805"
     CDS             complement(3232..3357)
                     /locus_tag="VFI55_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074752.1"
                     /translation="MLGLSDRAIQVHGGGGPDEVHTQTITRIGLGHPGAAWWTTS"
     gene            complement(3374..4204)
                     /locus_tag="VFI55_11810"
     CDS             complement(3374..4204)
                     /locus_tag="VFI55_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7074753.1"
                     /translation="MSRFTNVPVAPPNPVFGLAEGSRAVSELGWFVLARRWLARGRTG
                     DGRPVLVLPGQFADDWSTAPLRRLLSSLGYQAYGWRLGTNIGPTPHIVGGLDRLISEL
                     GDRHGGPVSVIGQSLGGFFGAELERRHPGAVDRLITLGSPVGITSPRQSRGARYYEWF
                     TSEHLPEYAFDLWTEAPPLSIPVTSIYSRSDGVVRWQACLYPEGPLTENIAVCSSHFG
                     MGFHPAAVYAVLDRLAVPVDNWHKFVAPKHLRAYFPAPNYLADERPHRSDTGPVSAAN
                     "
     gene            4467..6218
                     /locus_tag="VFI55_11815"
     CDS             4467..6218
                     /locus_tag="VFI55_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7074754.1"
                     /translation="MTDPTAAATSEDALSYSSAGTGFEAVMANAASAGSVLPLPELTR
                     FALSVARHPKPVASRIGRLAAQLVRIGRGKPAEAPGRADSRFRDRAWADNPLLNGVAL
                     SYLACCDALEGALDDADVDWRTRERLRLFIDNVLAAAAPTNNPLTNPAFWKEFIDTGG
                     RSVRAGVDNLVNDVTSPTKLPSSVDPSAYRIGENIAATPGKVVRRNRIYELIQYQPRA
                     DEIDEVPVLLVSSPVNKYYLVDLEPEQSVIRAELERGRSVFVASWVNPDANHADVGFD
                     DYVSSTIEMLETISDISGSVTTHLLGLCGGGLLAFMTAAYLAAIEQQHLLATLTIAIA
                     VLDYDRGPSAMAYLDQKQVDRAVRRAVKHGYFDARDSAWSFALIRPIEGIWTNVVNNY
                     LLGKPPPAVGMLYWAADQTNLATHFGAQMMEAALENTLTRPGEVSINGVPIDAGKITV
                     DTYVLAASTDHISPWQNCYRTVSMVGGDVTFVLARGGHAIAIARPPGSARASYRTGDI
                     SDTDPQTWLSGSVDNQGSWWDHWNAWIARHTPGKRPAPERLGSDTYPPVCDAPGEYVR
                     RVITFPAETSTDGRAEP"
     gene            6393..7157
                     /locus_tag="VFI55_11820"
     CDS             6393..7157
                     /locus_tag="VFI55_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005262694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7074755.1"
                     /translation="MTILDRFRLDGRVAIVTGASSGLGVGFAAALAEAGADLALGARR
                     VDRLAETCTLVENTGRKAIAVRTDVVDPDDCTALVDAAMTAYGRVDILVNNAGVGTAV
                     PALKETPDQFRSVIDINLNGCYWMAQACARVMLPGSSIVNISSVLGLTTTGLPQAAYA
                     SSKSGLIGLTRDLAQQWTSRKGIRVNALAPGFFASDMSDSMDRNVVETQILPRVLAGR
                     LGVHAELAAALIFLVSDAGSYVTGITLPVDGGVLTT"
     gene            complement(7233..>8241)
                     /locus_tag="VFI55_11825"
     CDS             complement(7233..>8241)
                     /locus_tag="VFI55_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HET7074756.1"
                     /translation="FWGSIIAFGKLQEILPGSPIGLGKAQQVVNVLLLAAAIVAAVVI
                     GLGAHPGSGGVSLWWMIGLLGAAGVLGLMVVLPIGGADMPVVISMLNAMTGLSAAAAG
                     LALNNTAMIVAGMIVGASGSILTNLMAKAMNRSIPAIVAGGFGGGGVAPPDSGSGDKH
                     VKATSAADAAIQMAYANQVIVVPGYGLAVAQAQHAVKDMASLLEDKGVPVKYAIHPVA
                     GRMPGHMNVLLAEAEVDYDAMKDMDDINDEFGRTDVAIVIGANDVTNPAARNEKSSPI
                     YGMPILNVDKARSVIVLKRSMNSGFAGIDNPLFYSDTTTMLFGDAKKSVSEVAEELKA
                     L"
BASE COUNT         1452 a         2750 c         2623 g         1416 t
ORIGIN      
        1 tcatcgactg cagcggctgc atggcgctca ttggagcctg catcagctgc gacggcgcct
       61 gcagcgcctg cgggaccgcg cccatcatcg actgcactgg ctccatgaaa gtgttcagct
      121 ggccgcccag gtcttgaccg gccgaggtcg gtgcgccggc accgtttgtc gccgagaacg
      181 cggcgcgcat cccgtcgccg gctccggcat cgcccgccgc ctggccgatc gccgaggctg
      241 cctcggctgg agccgcgggc gaggcaccca tggccgccaa cggcggagga attgccaggc
      301 tttccgccag cccggccaaa atccccccgt agctcgcgcc gaccgcggag ttattgggcc
      361 acatcacccc gaagtactcg gtgtccagcg cgacgattcg cggtgtcagc gtccacagca
      421 cactgggatt ggcaatgttg tcggccgccg attcgtcacg gttagcgttg cattccggtg
      481 ccgggcgcat accggcactg gctgtctcgt aggccgagat cgcggctgaa acgacggccg
      541 gcttcacgtc gacccagccg gccaacccgt gcaaggccgc gttgagcatc gtggcgttgg
      601 cggccgatcc cgcggatccg gccccggccc agcttgccga ggtcgcaacg gtgttgatca
      661 tggacgcgct tgctgaagcg tgatgactgg cgcccacggc gatccaggcc gtttggttgg
      721 ccaacatggt ggctaccccg gtcccggtct tgagcagcag atcgttcgct tcaggtgggc
      781 gcgccgccca cccggggtca ggcatcggcg tctcttacag tccgagcgtc aagctgcgcg
      841 ccgcctcggt ggcggtatac acgctcgacg cgacactctg cgtcccggcg aacagtccgc
      901 gctgcagcga gtgctcctga accactccca cgtaggcgcc gccgcacgcg ttcagcgccg
      961 tggtgaacag ggccgaatca aggtcattac ccatgggtgt tgtaccgagc aaggccggcg
     1021 cggcggccgc ggccgccgca gagatttccc cgctgatcgc cgattcagcg ccagctgaac
     1081 ccaagacagc ctcgggctgt acggtccacg acattatttc cctccgaatg ttctcgcggc
     1141 ccctccgccg cgaataatcg gccagcgtag tgcctgctga ccgatccccg tgttcacgaa
     1201 gatacgtgaa cacttggcac catacgctcg tattgtccgc tggtctggcg caatgcaaaa
     1261 ttcgctcggt ggtcatgttc gatgaaccca cacccacgcc gcacgcgctt tgttctgcca
     1321 ctttccgcac agagaccgct gcaatcactt ctgcaacgtc cgtcacctac cgtcggtgcg
     1381 tcaccagtcc ggcaaggaaa acggttcaaa ttggcaacgc tgggactccc gtgaaggacc
     1441 ccctcggatc atgtgggggt cggaagtttg agagcgttcg cccaaatcct cgtggatgtc
     1501 tcgacgagaa cttcaatcgg tgtgtcgtcg ccaagacaga agctgaaata ggccacgcgg
     1561 ctcatcatcg cggacagtgc ccgggatgct tggtaggcgt cgagtcgagg gtctgcgagg
     1621 ccgcgtttct gcagatcggc gatgacgtgg gcgttgcgca ccacaaaggc ttgaccgcgc
     1681 cgccggcgag tctcacggaa gttggggtcg gcgatagcga cctgttcgag cagcatcatc
     1741 agtttggcat tgcgctggta tgcccgcagg tatgcacgat tgctcgcttc gatgagcgcg
     1801 tacggcgaca tttcgctgtc gtcgtcaagc cgtgcaattc cggggtggag catatcgtct
     1861 tggacttgct cgaggaccgc ttgcaggatt tcgtccttgt tcgagaagta ggtgtagaac
     1921 gtgccggtcg agcacctcgc tgccttggtg atgtcggtga tctgggcgtc gaggtagccg
     1981 ctgcgttgga agacgcggcg cgcaccttcg ataagcgcgg ctcgcgttcg aacaccccgc
     2041 gcggtcggcg gcggatcgcg caacatcgac gtgtcggcca gaggagggaa gtcgatcgcg
     2101 taggtggccg tggcgtcagc gtggtccttc atcgtggaca tcgctttctc atcgccgctt
     2161 ccggcccgac cggtggtggc tacgtgttgg gggctcacag tccgtactcc ccgacaaggg
     2221 cccaggcgcg gcggagcaga tcctcagtgt acgccgcagg tggcgcgcca ctcggtgctg
     2281 cattggctgg ttcgtcggat attcgttttc ggattccttc gacgatgacc gcgagtttcc
     2341 agagtccgag aacatgccag aacccgagcg cggagacatc gcgtccggtc gtgtcggcat
     2401 aggtggcgat gatctcttcg cgggcagcga agccgggcaa cgtcgagggc gcgaatacgt
     2461 cggcgcagtc ggtttctccg ttgtgtggcc agtaggccag tagggtgccg aggtcggcga
     2521 gcggatcacc gagtgtggac aattcccagt cgagcgcggc ccgcacgttt ccggtcgcgg
     2581 gatcgcagat gatattgcgc gtatgcagat cgccgtggac cagacacagg ccgttcgact
     2641 ccggcagcct ctgcgtgagc aaatcggtga gccgatcgag atcgggcaag gcgcgtgtgc
     2701 ggctcgcgtc gagttggcgg gcccagcgcg acaattgccg cggtgcgtac ggcttgtggc
     2761 tggccaggtc gagcagttgc acgtcagcga gctcgacggc gtggacggcg gcaagcgcgc
     2821 gggccatcga cgctccgacc gatcggcggg ccgccgtggt cagcgactgt gcacttgcgg
     2881 ggtcctcgat gacgagtccg tcgacatact ccatcaacag atttggcacc ccatcgactg
     2941 tccacatgcc gaaaaccctg ggtaccggga cttcggagcc ctgtagggct gcgatgatcc
     3001 ggtgctcgcg gccgatatcg tgcgcggatt gcaggacgtg tccgcggggc gggcggcgca
     3061 ggacccactg ccgaccgtcg gtgtccgtga gccggtaagt caggttcgac tggccgtgtc
     3121 cgattcgccg cgccgaaagc ggtggtgaga gcacgtcgaa cgtcgcgatc cattcggcaa
     3181 cgaggtcgag acgggactcg gctgcgaccg tgtcgctggg tgcggcgtcg ctcatgaggt
     3241 tgtccaccac gcagcgccgg ggtgacccaa tccgatccgc gtgatcgtct gcgtgtgcac
     3301 ttcgtcgggt ccgccaccac catgcacctg gatggcgcgg tcgctgagtc cgagcacggt
     3361 cgcgcctccg gcctcagttt gccgccgaca ccgggccggt atccgagcga tgcggccgtt
     3421 cgtcggctag atagttcggc gccgggaaat atgcacggag gtgcttaggc gccacgaact
     3481 tatgccagtt gtcgaccggt acggccagcc ggtccagcac cgcgtagacg gcggccggat
     3541 ggaagcccat gccgaagtgg ctgctacata ccgcgatgtt ctcagtcaac ggcccctcgg
     3601 ggtacaggca ggcctgccag cgcaccacgc cgtcactcct gctgtagatc gacgtcaccg
     3661 ggatggataa cggaggcgcc tcggtccaca agtcgaacgc gtattcgggc agatgctcgg
     3721 acgtaaacca ctcgtagtag cgagcgccgc gagactggcg cggtgacgtg atgccgaccg
     3781 ggcttcccaa cgtgatcagc cggtcgacag cgccggggtg gcggcgctcc agctcggcac
     3841 cgaaaaagcc gccgagactt tgtccgatga cgctgaccgg accgccgtgc cggtccccaa
     3901 gctcactgat gagcctgtcg aggcctccga cgatatgtgg ggtcgggccg atgttggtgc
     3961 cgagccgcca cccgtaggcc tggtagccga gggaggacag caaacggcgc agcggggcgg
     4021 tcgaccagtc gtcagcaaac tgtccgggta gaacaaggac cggtcggcca tccccggttc
     4081 ggccccgcgc aagccaacgt cgcgcaagca cgaaccagcc caactccgac accgcccgcg
     4141 atccttccgc gagcccgaag accgggttcg gcggggcgac tggaacgttg gtaaaacgag
     4201 acatttgcgt cctccccagg cgagccggag ccgagcacgc ggcacatttc aggcacgtcc
     4261 aagacttgaa catgaaatta ggttcaacta tggtgagtgt gccatatttt gccccgatca
     4321 cacaatcttg tgtaggcgca gcagcagcgg aggaacgcca tggcggtggt ggaagcggtc
     4381 ggtgttcaaa tcgacgtcgg caccgagatt cagcagtatc gccccgtgcg gttccagttc
     4441 ctccgcaccg gggacgccgc gcagcgatga ccgatcccac cgcagcggcc actagcgagg
     4501 acgccctcag ctacagcagc gccggcaccg ggttcgaggc cgtgatggcc aacgcggcct
     4561 ccgccggcag tgtgcttccg ttacccgagc tgacccggtt cgcgttgtcg gtggcccgac
     4621 acccgaaacc ggtcgcctcg cggatcgggc gactcgcggc gcagctagtc cggatcggac
     4681 gcggcaaacc agccgaggcg cccggccgag cggacagccg cttcagggac cgcgcctggg
     4741 cggacaaccc gttgctgaac ggggtggcac tgtcctacct ggcgtgctgc gatgccctcg
     4801 aaggcgcgct cgacgacgcc gatgtcgact ggcgcaccag ggaacggctt cggctgttca
     4861 tcgacaacgt gctggccgcg gccgcgccga ccaacaaccc gctgacgaat ccggctttct
     4921 ggaaggaatt cattgacacc ggcggcagga gcgtgcgagc gggcgtggac aaccttgtca
     4981 acgacgtgac ctcacccacc aagttgccga gcagcgtcga tccgtcggcc taccggatcg
     5041 gcgaaaacat cgccgccaca cccggaaagg tggtccgacg gaaccggatc tacgagctga
     5101 tccaatacca gccgcgcgcc gacgagatcg acgaggtccc ggtgctgctg gtctcgtcgc
     5161 cggtcaacaa gtactacctg gttgacctcg agcccgagca gtccgtcata cgcgcggagt
     5221 tggaacgcgg ccgcagcgtt tttgtggcgt cctgggtcaa tcccgacgcc aaccacgccg
     5281 acgttggctt cgacgactac gtctcgagca ccattgaaat gctggaaacg atctccgaca
     5341 tcagcggctc cgtcaccacc catctgctcg gcctgtgcgg cggcggcttg ctcgccttca
     5401 tgaccgcggc atacctggcc gcgattgagc aacagcacct gctcgcgaca ctgaccatcg
     5461 ccatcgccgt cctcgactac gaccggggcc catcggccat ggcgtatctc gaccagaagc
     5521 aggtcgaccg cgcggtgcgt cgcgcagtca agcacggcta cttcgacgcc cgggactcgg
     5581 cgtggtcgtt cgcgctgatc cggcccatcg aaggcatctg gacaaatgtt gtgaacaact
     5641 acctgctcgg caagccgccg cccgccgtgg gaatgctcta ctgggcggct gaccaaacaa
     5701 atttggccac ccacttcggc gcgcagatga tggaggcagc gctggagaac accctcaccc
     5761 ggcccggcga agtcagcatc aacggcgtgc cgatcgacgc cggaaagatc accgtcgaca
     5821 cctacgtgct cgccgcgtcg accgaccaca tctccccgtg gcagaactgc tatcggacgg
     5881 tctcgatggt cggcggcgac gtgacattcg tgctcgcacg gggcggacac gccatcgcga
     5941 tcgccagacc gccgggcagc gcacgcgcca gctaccgaac gggcgacatc agcgataccg
     6001 atccgcagac ctggctctcc ggctcggtcg acaatcaggg cagctggtgg gaccactgga
     6061 acgcgtggat cgcacggcac acccccggca agcggccggc acccgagcga ctgggaagcg
     6121 acacgtaccc gccagtctgt gatgcaccgg gcgaatatgt gcgccgcgta ataaccttcc
     6181 ccgccgagac atccacggat ggtcgggccg aaccatgact ggacgagtcg agcccgcgac
     6241 gcatttgacc tcgagtcttt cctgatgctg cccacctcaa aacggcatgc gggcaccact
     6301 cggcgtcgaa ctcggctccg accacacgcg agccctcgac gttgccgggg ccgcctggat
     6361 catcgcgcaa acgaccaccg cgaaaggaag ccatgaccat cctcgaccga ttccgactcg
     6421 acggtcgcgt cgccatcgtg accggcgcat ccagcggcct gggcgtcgga tttgctgccg
     6481 cgctcgccga agccggcgcc gacctcgccc tcggcgcccg ccgagtggac cgcctcgcag
     6541 aaacctgcac ccttgtcgaa aacaccggac gcaaggcgat cgctgtacgc acggacgtcg
     6601 tggaccccga cgattgcaca gccctcgtcg acgcggccat gaccgcctac gggcgcgtcg
     6661 acattcttgt caacaatgcc ggagtcggca ccgccgtccc cgcactgaag gaaacccccg
     6721 accagttccg atcggtcatc gatatcaacc tcaacggctg ctactggatg gcacaggcct
     6781 gcgcgcgcgt catgctcccc ggcagttcga tcgtcaacat atccagtgtg ctgggactga
     6841 ccaccactgg cctgccgcaa gccgcatacg cgtcatcgaa gtccggcctc atcggactca
     6901 cccgcgatct tgcccagcaa tggacctccc gcaagggaat tcgcgtgaac gcgctcgctc
     6961 ccggcttctt cgcttccgac atgagcgaca gcatggaccg taacgtcgtc gagacacaga
     7021 tcctcccgcg tgtcctcgcc ggacgcctcg gcgtccacgc cgaactcgcc gccgcactga
     7081 tattcctcgt cagcgacgcc ggaagctatg tcaccggcat aaccttgccg gtcgatggcg
     7141 gagtgctgac aacctgacac cgagtccggc cgttgccgtt gagcacttct cggacggttt
     7201 atgcctgatg tgccgcaacg gacctgtcgt cgttatagcg ccttgagttc ttcggcgact
     7261 tccgacacgg attttttggc gtcgccgaag agcatggtgg tggtgtcgct gtagaacagt
     7321 gggttgtcga tgccggcgaa tccggagttc atcgagcgtt tgagcacgat gaccgacctg
     7381 gccttgtcca cattcaggat gggcatgccg tagatggggc tggatttttc gttgcgggct
     7441 gcggggttgg tgacgtcgtt ggcgccgatc acgatggcga cgtcggtgcg gccgaattcg
     7501 tcgttgatgt cgtccatgtc tttcatcgcg tcgtagtcga cttcggcttc ggccagcagc
     7561 acgttcatgt gtccgggcat ccgcccggcc acggggtgga tggcgtattt gaccggcacc
     7621 cctttgtctt ccagcaggga ggccatgtct ttgacggcgt gctgggcctg ggccacggcc
     7681 agcccgtagc cgggcaccac gatcacctgg ttggcatagg ccatctggat cgcggcatcc
     7741 gcggccgagg tggccttgac atgcttatcg ccgctaccgg agtcgggcgg ggcgaccccg
     7801 ccgccgccga acccgccggc cacgatcgcg ggaatggagc ggttcatcgc cttggccatc
     7861 aggttggtca aaatcgaccc cgaggcgccc acgatcatcc cggccacgat catcgcggtg
     7921 ttgttcaacg ccaaccccgc cgccgccgct gagagcccgg tcatcgcgtt gagcatcgag
     7981 atgaccaccg gcatgtcggc cccgccgatc ggcaacacca ccatcagccc caacaccccg
     8041 gccgcgccca gcagcccgat catccaccac aacgacaccc caccactgcc gggatgggcg
     8101 cccagcccga tcaccaccgc cgcgacaatg gccgcggcca acagcagcac gttgaccacc
     8161 tgctgcgcct tgcccagccc gatcggggaa ccgggcagga tttcctgcaa cttgccgaac
     8221 gcaatgatcg acccccaaaa c
//
LOCUS       DASSGQ010000268         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_869761,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000268 DASSGQ010000000
VERSION     DASSGQ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869761"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFI55_11830"
     CDS             complement(<1..201)
                     /locus_tag="VFI55_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074757.1"
                     /translation="MSNPSGFSQPYAGSPKYEYLAPSQVGNSDQLHLSIGQPEADRIA
                     DRIGLNKAHAFTDAPTRQLDHAL"
     gene            complement(619..1377)
                     /locus_tag="VFI55_11835"
     CDS             complement(619..1377)
                     /locus_tag="VFI55_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET7074758.1"
                     /translation="MRLATFNILHGHSPSDETVDVARYADAIKALDADVLALQEVDRD
                     QPRSEHADLTAIAADAMGAVEHRFVAALAGTPGSAWVAATGEEQPGTATYGIALLSRY
                     PVRSWQVVRLPSIPFRVPMWLRYPRKFLVVSEEPRVAMAGVIDSPLGVLTIATTHLSF
                     VPGWNVHQLRRLRRDLTPLPDPLVLLGDLNLTCRSAARVTGYRPLTDVATFPTDAPTR
                     QLDHALLRGRLGPVRYQSSRQLPLSDHRALIVDL"
     gene            1416..2003
                     /locus_tag="VFI55_11840"
     CDS             1416..2003
                     /locus_tag="VFI55_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074759.1"
                     /translation="MADEREARRAALRRVAGLLKRTGLPFALAGSYGLWAHGAPESAH
                     DVDFVIAEEHTEQIATDLTAAGLDVIRPPEDWLLKVSTDGVTVDLLHRLARRPATVEM
                     LARSHVFEVLSVEMPVLDATDLLSSKLGALTEHFCDFAPLLTAARAVREQVDWARVRA
                     ELADHDFAVAFLFLADRLGITDRAAAAANPVAAAS"
     gene            complement(2016..>2672)
                     /locus_tag="VFI55_11845"
     CDS             complement(2016..>2672)
                     /locus_tag="VFI55_11845"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7074760.1"
                     /translation="VSGYAGLPVVDSCGSATVRERSVRMITGKRPGYGRSTRLPGRGF
                     AEHADDMARVLDHLDLDRVFVCGQSGGAPHALAFAARHPGRLRAASIRSGMAPLTDED
                     ATQVIPINQQARRLVAAHDVDGLRTLLTPIAAEMLADSLAGYAAQMATAPPEDHTVMT
                     DPLWQQGYIRATREALGAGFDGWVDETLAILGDWPDVPLPAVTTSLVWSHARRGCCVL
                     "
BASE COUNT          437 a          902 c          914 g          419 t
ORIGIN      
        1 cagcgcatga tcgagctgtc gggtcggcgc gtcggtgaac gcgtgcgcct tgttcagtcc
       61 gatgcggtcg gcaatccgat cggcctctgg ttgcccgatc gacaagtgga gttgatccga
      121 gttcccgact tggctgggtg ccaggtactc gtacttcggt gacccggcgt agggctggga
      181 gaatccactt ggattcgaca cggtcggacg ctagcgcgtt gccgaagcct cgtcgagtgc
      241 ccttcgcgtc gcccacccgg ccccgacact gtctcgatca agggctagtc gtgtgtgccg
      301 gtacgcggct ggtccgccgt agacgccggt gagagtggtc tagaccatct gccccggcaa
      361 cagatcgcaa gctggccctc cgggcgggtg gacagccagc cgattcccca ccagcgcgca
      421 tttgtcactc gttgcggtgg gcggccctca tcggcgctaa ctttcgctac gcgtcgacct
      481 atcgggttga gtcttgacac caacccgcgc ccgacgttgc ccaaaggcct gctatcaagg
      541 ccgaaaacgt cacaagacag ccattgacac cggcgagctc ggtcagggat gaccccatgt
      601 tgtggtgggt tgtgacggtt acaggtcgac gatcagcgca cgatggtcag acaggggcag
      661 ctgtcggctg gattggtagc gcaccggccc gagccgccct cgaagcagcg catgatcgag
      721 ctgtcgggtc ggcgcgtcgg tgggaaatgt cgccacgtcg gtcaacggtc ggtagccggt
      781 gacgcgcgcg gcggaacggc aggtcaggtt gagatcgcct agcagtacga gaggatccgg
      841 cagcggggtc aggtcgcggc gtagccgccg tagctggtgg acattccaac ccggcacgaa
      901 cgacaggtgc gtggtggcga tggtcagtac accgagcggg ctgtcgatga cgccggccat
      961 cgccacccga ggctcttcgg agacgaccag gaatttgcgc gggtagcgca gccacattgg
     1021 cacgcggaac gggattgacg gaagtcgaac tacctgccat gaccgcaccg gatatcgcga
     1081 gagcaacgcg atgccgtagg tggccgtgcc cggttgctcc tcaccggtgg ctgcgaccca
     1141 ggctgatccc ggcgtaccgg cgagcgcggc gacgaaccgg tgctccaccg cgcccatcgc
     1201 gtccgccgcg atcgcggtca gatcggcgtg ctcggaacgc ggctggtcgc gatcgacttc
     1261 ttgtagggcc agcacgtcgg cgtccagggc cttgatggcg tcggcgtagc gcgcgacgtc
     1321 gaccgtctcg tcggatgggg agtgcccgtg caggatattg aaggtggcca gccgcacgtc
     1381 actactctta ccgcatcggc ggcgagggag tagccatggc ggacgagcga gaggcgcgac
     1441 gcgcggcgct gcgccgggtt gcgggcctgc tcaagcgcac cggcctgccg ttcgcgctcg
     1501 ccggaagcta cgggttatgg gcacacggcg cgccggagtc ggcgcacgac gtggacttcg
     1561 tcatcgccga ggaacacacc gagcagatcg cgaccgacct caccgccgcc gggctagacg
     1621 ttatccggcc acccgaggac tggttactca aggtgtcgac cgacggggtg accgtcgacc
     1681 tgctgcaccg actcgcgcgc cgaccggcca cggtcgagat gctggctcgc agccacgtgt
     1741 tcgaggtgct gtcggtggag atgccggtgc tcgacgccac cgacctgctg tccagcaagc
     1801 tcggagcgct gaccgagcac ttctgcgact tcgcgcccct gctcacggcc gcccgcgcgg
     1861 tccgcgagca ggtcgactgg gcacgcgttc gcgcagagct cgccgatcac gacttcgcgg
     1921 tggccttcct cttcctggcc gaccgcctcg ggataaccga ccgcgccgcg gccgcagcaa
     1981 atcccgtcgc cgctgcgtcc tgaccaccgc ggcgtctata acacgcagca tccccggcgg
     2041 gcgtgggacc acaccaggga cgtggtgacc gcgggcagcg ggacgtcggg ccaatcgccg
     2101 aggatcgcca gtgtctcatc gacccatccg tcgaagccgg cgcccaatgc ctctcgagtc
     2161 gctcggatgt agccctgctg ccacagggga tcggtcatga cggtgtggtc ttccggcggc
     2221 gcggtggcca tctgggcggc atagccggcc agcgagtcgg cgagcatctc ggccgcgatc
     2281 ggcgtaagca gcgtgcgcag cccgtccacg tcgtgcgcgg ccaccaggcg acgcgcctgc
     2341 tggttgatcg ggatcacctg cgtcgcgtcc tcatcggtca acggcgccat cccggaccgg
     2401 atcgacgcag cccgaagccg gcccggatgg cgggcggcga acgcgagcgc atgcggcgcg
     2461 ccgccgcttt gcccgcacac aaacacccga tccaggtcga gatggtcgag cacgcgcgcc
     2521 atgtcatcgg catgctcggc gaaaccccgg ccaggcagcc gcgtcgaacg accgtatccc
     2581 ggacgcttac cagtgatcat ccgcaccgaa cgctcccgca cggtcgccga tccgcacgaa
     2641 tcgaccaccg gcagcccggc gtacccggaa ac
//
LOCUS       DASSGQ010000269        19582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_717554,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000269 DASSGQ010000000
VERSION     DASSGQ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 19582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19582
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717554"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VFI55_11850"
     CDS             complement(<1..472)
                     /locus_tag="VFI55_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7074761.1"
                     /translation="MAYHNPFIVNGRIKFPENTNLVRHVEKWAKVRGDKLAYRFVDYS
                     TERDGIYRDISWSAFSARNRAVGARLQQVTQPGDRIAILCPQNLDYLSALFGALYAGR
                     IAVPLFDPSEPGHVGRLHAVLDDCTPSTILTTTEAAEGVRKFIRARSAKERPRVI"
     gene            complement(822..1835)
                     /locus_tag="VFI55_11855"
     CDS             complement(822..1835)
                     /locus_tag="VFI55_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HET7074762.1"
                     /translation="MTTTKARRKRHRILAWVAALAVAGVVLLVVLAVVVLLRSRESPP
                     SAVPPGVLPPASTQSHPHKPRPASQDASCPDVDLIAVPGTWESSPQDDPLNPMQFPNA
                     LLRKVTGPISQQFPTSRVQMYTVPYTAQFHNPMSGDTQMSYNDSRAEGTRAMVQALTD
                     MASKCPLTSYVLVGFSQGAVIAGDIASDIGNGRGPVDDDLVLGVTLIADGRRQTGVGN
                     DIGPNPPGQGAEITLHEVPVLSGLGLTMTGARPGGFGALDSKTNEICAPGDLICAAPD
                     EAFSVANLPNTLNTLAGGAGQPVHALYATTQCWNLDGAPATDWTLNWTHGLIENAPHP
                     KHG"
     gene            complement(1966..2880)
                     /locus_tag="VFI55_11860"
     CDS             complement(1966..2880)
                     /locus_tag="VFI55_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_178017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HET7074763.1"
                     /translation="MRGLSVLLRVFCVAALSVGVGILTCALAVTGAAGKARAAGYESL
                     MVPSAAMGRDIPVAFLAGGPHAVYLLDPFDAAPDVSNWVTAGNAMNTLGGKGISVVAP
                     AGGAYSMYTNWEQDGSKQWDTFLSSELPDWLAANRGLAPGGHAAVGASQGGYAAMALA
                     AFHPDRFGFAGSLSGFLYPSNTTTGGAILAGLQQFGGVDGNGMWGAPQLGRWKWHDPW
                     VHASLLAQNNTRVWVYSPTNDSASDPAAMIDQAAEAMGNSRTFYQQYRSVGGHNGHFE
                     FPGGGDNGWGSWSGQLGAMSGDIVGAIR"
     gene            complement(3059..4081)
                     /gene="ag85A"
                     /locus_tag="VFI55_11865"
                     /gene_synonym="fbpA"
     CDS             complement(3059..4081)
                     /gene="ag85A"
                     /locus_tag="VFI55_11865"
                     /gene_synonym="fbpA"
                     /EC_number="2.3.1.122"
                     /EC_number="2.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol
                     acyltransferase/mycolyltransferase Ag85A"
                     /protein_id="HET7074764.1"
                     /translation="MTLVDRFRGAVAGMPRRFVVGAVGAALLSGLIGAVGGSATAGAF
                     SRPGLPVEYLQVPSAAMGRDIKIQFQSGGANSPALYLLDGMRAQDDYNGWDINTPAFE
                     WYYQSGISVVMPVGGQSSFYSDWYKPACGKAGCTTYKWETFLTSELPQYLSAQKQVKP
                     TGSAAVGLSMAGSSALILAAYHPDQFIYAGSLSALLDPSQGMGPSLIGLAMGDAGGYK
                     KDDMWGPTDGGAWQRNDPSLQVGKLVANNTRIWVYCGNGKPSDLGGDNLPAKFLEGFV
                     RTSNLKFQDAYNAAGGHNAVFNFDANGTHDWPYWGAQVNAMKPDLQATLGATPNTGGG
                     ADNSGT"
     gene            complement(4468..6456)
                     /gene="aftB"
                     /locus_tag="VFI55_11870"
     CDS             complement(4468..6456)
                     /gene="aftB"
                     /locus_tag="VFI55_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminal
                     beta-(1->2)-arabinofuranosyltransferase"
                     /protein_id="HET7074765.1"
                     /translation="MPSASPRLKAFEVAVLRRHRPVVRRVGRPLFPYGTVVRISLWTS
                     VAAVAVLFAWGSWRRRWIADDGLIVLRTVRNLLAGNGPVFNQGERVEANTSTAWTYLM
                     YAGSWVGGPMRMEYVALALALVLSVLGVALLMLGTGRLYAPSLRGRKAIMLPAGALVY
                     IALPPARDFATSGLESGLTLAYLGLLWWMMVRWSQPVRGRADSPAFLGALAFVAGFSV
                     LVRPELALMGGLALIMMLVAARNWRRRALIIAAGGSLPVAYEIFRMGYYGLLVPGTAL
                     AKDAAGDKWSQGMTYLSNFNAPYAVWIPLVLVVPLGVLVLAARRRPSFLRPTAAPGYG
                     RLARAAQSPPAVVAFVLVSGLLQALYWIRQGGDFMHGRVLLAPLFCLLAPVAVIPVAI
                     PDGEDYSRETGYWVTAAAGLLWLGIAGWSLWAANSPGMGDDATHVSYSGIVDERRFYA
                     QATGHAHPLTAEDYLDYPRMAAILTALDNTPDGALLLPSGNYIQWDLVPQLQPPPDSS
                     GGNKAPQKPQHAVFFTNLGMVGMNVGLDVRVIDQIGLANPLAQHTERLKHGRIGHDKN
                     LFPDWVIADGPWVKVHPGIPAYLDENWVAQAVAALQCPETQAVLSSVRAPMSLHRFIS
                     NVVNSFEFTGYRIDRVPLYELARCGLPVPQPIPPPPHE"
     gene            complement(6443..7360)
                     /locus_tag="VFI55_11875"
     CDS             complement(6443..7360)
                     /locus_tag="VFI55_11875"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HET7074766.1"
                     /translation="MSEDTENVVTGPPANLIAGVIKAMRPRQWVKNVLVLAAPLAALG
                     GPVRYDYAEVLSKVSVAFVVFCMAASSIYLVNDARDVEADREHPTKRFRPIAAGVVPE
                     WLAYTLSVVLAVASLAIAWSVTPNLALVMAVYIVMQLAYCFGLKHQAVMDICIVSSGF
                     LIRAIAGGVAANIPLSQWFLLMMAFGSLFMVAGKRYAELQVAERTGAAIRKALESYTS
                     TYLRFVWTMSATAVVLCYGLWAFERDRHSGSWFAVSMVPFTIAILRYAVDVDGGLAGE
                     PEDIALRDRVLQLLALAWIATVGAAVALS"
     gene            complement(7373..7903)
                     /locus_tag="VFI55_11880"
     CDS             complement(7373..7903)
                     /locus_tag="VFI55_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7074767.1"
                     /translation="MPESLPADTPRGEVAALVAVQSALTDRPGVLPVARVLSHFGEHS
                     IGWVAVSLLGAVLMPRRRRDWLVAGAGAFTAHAAAVLLKRLVRRKRPHHAAVAVNVGT
                     PSQLSFPSAHATSTTAAAILMGRVTGLPLPAVLVPPMAISRMVLGVHYPSDVAFGIAL
                     GAAVAAIAVRLDSGLD"
     gene            complement(7896..9851)
                     /locus_tag="VFI55_11885"
     CDS             complement(7896..9851)
                     /locus_tag="VFI55_11885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7074768.1"
                     /translation="MTAVSLLARIILPRPGEPLDVRKLYLEESSTNARRAHATSRTSL
                     QIGAESEVSFATYFNAFPASYWRRWSICQSVVLRVEVSGAGRVDLYRTKATGARIFIE
                     GHQFAGSEDEPQVLEIEVPLKPFEDGGWIWFDITTDSKVTLVSGGWYAAEPAPGTANI
                     AVGIPTFNRPNDCVNALRELTADHLVDKVIGAVIVPDQGVRKVRDHPDFPAAAARLGN
                     RLSIHDQPNLGGSGGYSRVMYEALKNTDCQQILFMDDDIRIEPDSILRVLAMHRFAKT
                     PMLIGGQMLNLQEPSHLHIMGEVVNPSNFMWTAAAHAEYDHNFAEYPLNDDNARSKLL
                     HRRIDVDFNGWWTCMIPRQVAEEMGQPLPLFIKWDDAEYGLRAGEHGYPTVTLPGAAI
                     WHMAWSDKDDAIDWQAYFHLRNRLVVAALHWDGDVTGLVRSHLKATLKHLACLEYSTV
                     AIQNRAIDDFLAGPEHIFSILESALPEVHRMRKEYPDAVVLPAASELPSPLHKTKAMK
                     PPVNPLSIGYRLARGIVHNMTPADPDAHQRPEYNVPTQDARWFRLCTVDGVTVTTADG
                     CGVVYRQRDRRKMFSLLLQSLRRQRQLASRFDEMRKLYRDALPVLSSKQKWETALNPA
                     PKPAPKLAKSPASGWDPQPAADKPEHA"
     gene            complement(9848..11044)
                     /gene="glf"
                     /locus_tag="VFI55_11890"
     CDS             complement(9848..11044)
                     /gene="glf"
                     /locus_tag="VFI55_11890"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003913521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HET7074769.1"
                     /translation="MTARFDLFVVGSGFFGLTIAERVANQLGKRVLVVDRRPHIGGNA
                     YSEAEPQTGIEVHKYGAHLFHTSNKRVWDYVRQFTEFTDYRHRVFAMHNGQAYQFPMG
                     LGLVSQFFGKYFTPDEARQLIAEQAAEIDTADAQNLEEKAISLIGRPLYEAFVKGYTA
                     KQWQTDPAKLPAAVINRLPVRYTFDNRYFSDTYEGLPQDGYTAWLTNMAADDRIEVRL
                     NTDWFDVRDQLRADNPDAPVVYTGPLDRYFDYADGRLGWRTLDFEVEVLPIGDFQGTP
                     VMNYNDLDVPYTRIHEFRHFHLERDYPTDKTVIMREYSRFAEHDDEPYYPINTEADRA
                     LLAAYRARAKSETASSKVLFGGRLGTYQYLDMHMAIASALSMYDNVLAPHLQDGAPLA
                     EEGALE"
     gene            11312..12079
                     /locus_tag="VFI55_11895"
                     /pseudo
     CDS             11312..12079
                     /locus_tag="VFI55_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218327.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PirG"
     gene            12513..14192
                     /locus_tag="VFI55_11900"
     CDS             12513..14192
                     /locus_tag="VFI55_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LGFP repeat-containing protein"
                     /protein_id="HET7074770.1"
                     /translation="MSSRHRAPTMLLTAIAATVVIVSWVGDAVRDPGAPDPPRARGTQ
                     LTEQPLTGLGAGVTVREVSQDTPFSLVALTGDLAGTSTRVRAKRPDGSWGPWYRTEYE
                     TAAPDASPAAGTGPAGAVRPAGSSEGPRSTDPVFVGTTRTVQIAITRPLDAPVTLGAA
                     PASVPTPQDGLGYKPMSKEQPYAQNISAILISPPQAPANAQWTPPSGVLMPGQPPSII
                     SRAEWGADESLRCGSPQYDNGIRAAVVHHTAGSNDYSPLESAAIVKAIYTYHSKTLGW
                     CDIAYNALVDKYGQVFEGSSGGLTKAVEGFHTGGFNRNTWGVAMIGNFDDVPPTPIQL
                     RTVGRLLGWRLGLDGVDPKGTVALESAGSHYTTYPAGAIANLPAIFTHRDVGNTDCPG
                     NAAYALMDEIRDIASHFNDPPEELIKALEGGAIYEHWQAIGGMNSVLGAPTSPEDDAD
                     GDARYATFAKGAMYWSPETGPQPVTGAIYDAWASLSYERGPLGLPTSAELQEPLQITQ
                     NFQHGTLNYERLTGNVTEVVDGITTPLSTQSPSGPTVPPEHFSLPSHPTVT"
     gene            14415..16070
                     /locus_tag="VFI55_11905"
     CDS             14415..16070
                     /locus_tag="VFI55_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HET7074771.1"
                     /translation="MVTAPELMTAAAGNLAGIGSTLEEATSAAAGPTTGVASAAADEV
                     SVAVSQLFGTYAQEFQTVSGQAATFHNEFVSLLAGGGAAYLSADIANAEQSLLGQLPS
                     AGVVSALANGGAASLLSDRIATGAQAASAAIAGTPALRGLETALLPGLFAPGAAAATG
                     PGGAYRQLLANTGTNLESLFGTWSAHPFPLLNQIAANQRGYWLEIATALAGGIQNLPA
                     ELANLPQTIQAGVQGLLAFNAAFYVQQYITTQIGFAQTFVTNLDGAVTGIVDGLPNFV
                     AGLQTALHAVRAGDYFGAVQDVAQAYANLLVTGLNPGNLTVDLLTPLVNPTVVATTNP
                     TLLGPLGNLFAIANIPGQEAQYFTDLMPPSILRQMSQNFTNVLDALTIPSISATATLP
                     LLNPTAGSLSASFGLPLVLTYAVAGAPVATLNGVATSATAVQQALLAGDGLGAFNAFV
                     DAPAVIANSFLNGEVLVDFTVPVPVNLPAPLPSFTVPIILHLPFDGILVPPHPVTATI
                     DLNGFVPGLEIPATIFGTPFMGLVPLLVNYVPQQLAAAITPAA"
     gene            complement(16123..16938)
                     /locus_tag="VFI55_11910"
     CDS             complement(16123..16938)
                     /locus_tag="VFI55_11910"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7074772.1"
                     /translation="MTLPALIACDVDGTLFDESESITPRTRAAVRAAVAAGAQFVVAT
                     GRPPRWIRPVVEALGFAPTAVCANGAVIYDPANDRVVSVRTLGVDTLAELAELASRVI
                     PGAGLAVERIGDRAHDTATPQFISSPGYEHAWLNPDNTEVSIDDLLSAPAIKLLIRKT
                     GARSADMAAELAKHVGIEGDITYSTNNGLVEIVPLGISKASGVAEIAGPLGITSADVV
                     AFGDMPNDIPMLLWAGHGVAMGNAHPDVLAAADEVTTPNSDDGVGRVLERWWL"
     gene            complement(16961..17746)
                     /locus_tag="VFI55_11915"
     CDS             complement(16961..17746)
                     /locus_tag="VFI55_11915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009955783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7074773.1"
                     /translation="MAEPFYRLCEWVIPPVVAMNGTKFTFQGLENIPVQGGALLAQNH
                     TSYLDWLPSMFAVRERHRRRYFMIKAEMADVKAVNYVIKHARLIPVDRRKGHDALEVA
                     VQRLRAGELIGMHPEATISRSYELREFKTGAARMAVEAEVPIIPMIVWGVQRIWPKDH
                     PKKLFRNKVPITVAARRPLPPEGTPEQLNATLRQAMNAMLYRVQAEYPHPEGEYWVPE
                     RLGGSAPTQDDSRALRVAELVERVEKYGTDGVTRPGQTPRGSR"
     gene            complement(17764..18519)
                     /locus_tag="VFI55_11920"
     CDS             complement(17764..18519)
                     /locus_tag="VFI55_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7074774.1"
                     /translation="MPEPTFRAIEIVARLGVLASGSRITYRGEENIPDRGAAVIAINH
                     TSYVDFLPAALAVYRRHRRLRFMIKAEMQQVKVVNFLINHTGTIAVDRCAGAEAYAVA
                     VQRLREGELVGVYPEATISRSFELKEFKTGAARMAIEGNVPIVPVIVWGAQRIWTKDH
                     PRHLGRNRIPITVQVGAPLCATKDVAATDAALRESMATLLHQTQRRYPHPAGAYWVPR
                     RLGGGAPTLAEAARMDADELAARAAGRTPRQSR"
     gene            complement(18523..19293)
                     /locus_tag="VFI55_11925"
     CDS             complement(18523..19293)
                     /locus_tag="VFI55_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET7074775.1"
                     /translation="MEPVYATVIQLVRLLWRVQGLTITVTGVENLPTSGGAVIAINHT
                     GYLDFTFAGLPAYQQGLGRKVRFMAKQEVFDSKITGPLMRSVRHIPVDRQNGAASYEA
                     AVRHLKDGELVGVYPEATISRSFEIKEFKSGAARMATEAGVPIVPHIVWGAQRIWTKG
                     HPKKLWRPKVPITVLVGEPIEPTLGVEELKSLLHSRMQHLLERAQEMYGPHPAGEFWV
                     PRRLGGGAPSLAEATRMDAEEAAARAARRAQATGPPEQ"
     gene            19447..>19582
                     /locus_tag="VFI55_11930"
     CDS             19447..>19582
                     /locus_tag="VFI55_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074776.1"
                     /translation="MQVTSVGHAGFLIQTHAGNILCDPWVNPAYFASWFPFPDNSTLD
                     W"
BASE COUNT         3273 a         6698 c         6365 g         3246 t
ORIGIN      
        1 cgataacgcg gggccgctcc ttggccgatc gggcgcggat gaacttgcgg acgccttccg
       61 cggcctcggt ggtggtcaag atcgtggacg gcgtgcagtc gtcgagcacg gcgtgcaggc
      121 ggccgacgtg gcccggctca gaagggtcga acaacggcac cgcgatcctg ccggcgtaga
      181 gcgcaccgaa gagggcggac aggtagtcga ggttctgcgg gcacaggatg gcgatgcggt
      241 caccgggctg ggtgacttgc tgtagccggg cccccaccgc gcggttacgg gcgctgaagg
      301 ctgaccagga gatgtcgcgg tatatgccgt cgcgttcggt ggagtagtcc acgaatcgat
      361 aggccagctt gtcgccacga accttcgccc atttctcaac gtgacggacc aggttcgtat
      421 tttcggggaa cttgatcctg ccgttcacga tgaacgggtt gtggtacgcc atgccgcctt
      481 ctcctgtcac aaccatctcg gtcggcttcg ccgacatcta aatacacctc ggtcggcttc
      541 gccgacccca atacgtctac gcgggccggt cctcgcacgg caggcgcgtg gcggcgcccg
      601 tctattacgg ctacggcccc gatcgggcca cgacgaagtt gtactgctct taattttctc
      661 ttaatgttaa gggtcgcctg gcggcagacc aaatcacaag gtaccgccga tgtcgcccgc
      721 cgagcagcta ggttgcggct gtgtccaccg ctcgctggtc ggcaggtcgc cgagtgcccc
      781 accgcgggcc gatccggccc gcccgcatcg cccgggccag gtcacccatg tttggggtgc
      841 ggcgcgttct cgatgagtcc gtgggtccaa ttcagcgtcc aatcggtggc gggcgccccg
      901 tccagattcc agcactgagt cgtcgcatac agcgcgtgga ccggctggcc ggcgccgccg
      961 gccagcgtgt tcagcgtgtt cggcaggttg gcgacgctga acgcctcgtc cggggccgcg
     1021 cagatgagat cgcccggcgc gcagatctcg ttggtcttgc tgtcgagcgc gccgaaacca
     1081 cccggccggg cgcccgtcat cgtcaggccc agcccggaca gcaccggcac ctcgtgcaag
     1141 gtgatctcgg cgccctggcc cggcgggttg ggtccgatat cgttgcccac ccccgtctgg
     1201 cgacgaccgt cggcgatcaa cgtcacaccg agtaccaggt cgtcatcgac gggtccgcgg
     1261 ccattgccga tatcgctggc gatatcaccg gcgatcaccg cgccctggga gaagccaacc
     1321 agcacgtagc tggtcagcgg gcatttgctg gccatgtcgg tcagcgcctg aaccatcgcg
     1381 cgggtgcctt cggcgcggct gtcgttgtag gacatttgcg tgtcaccgct catcggattg
     1441 tggaactgcg ccgtgtaggg cacggtgtac atctgcaccc gggacgtggg gaactgctgg
     1501 gagatcggcc cggtcacttt gcgtagcaac gcattcggaa actgcatcgg gttgagtgga
     1561 tcgtcctgcg gcgaggactc ccaggtgccg ggaaccgcga tcaggtccac gtcgggacac
     1621 gacgcgtctt gggaagcggg gcgcggcttg tgcgggtgac tttgggttga cgcagggggc
     1681 agcaccccgg gcggcaccgc gctgggcggc gattcgcgac tgcgcagtag gaccaccaca
     1741 gccaggacga ccaacaacac gacgccggcc actgcgagcg cggcaaccca ggcgaggatt
     1801 cggtggcgct tacgccgggc tttcgtggtc gtcatgctct cccgctagca ggttcggtgg
     1861 gtcgcagctc gataccaggc aagcggccgt cccgccctgt gctgcttcct tacacggtac
     1921 cggcccgtcc cgcggcgccg tccgtgctgc tagcgcgccg cgcgactacc ggattgcgcc
     1981 gacgatgtcc cccgacatgg cgccgagttg accagaccac gagccccagc cgttgtcacc
     2041 gccacccggg aactcgaagt ggccgttgtg gccgcccacg ctgcggtact gctggtagaa
     2101 cgtgcggctg ttgcccatcg cctcggcggc ctggtcgatc atcgcggcgg gatcgctggc
     2161 gctgtcgttg gtcgggctgt aaacccacac gcgggtgttg ttctgcgcga gtagcgaggc
     2221 gtgtacccag gggtcgtgcc atttccagcg gcccagctgc ggggcgcccc acattccgtt
     2281 gccgtccacg ccgccgaatt gctgcaggcc ggccaggatc gcaccaccgg tggtggtgtt
     2341 cgacgggtac aggaacccgg acagcgagcc ggcgaagccg aagcggtcgg ggtggaaggc
     2401 ggccagtgcc atcgcggcat agccaccctg agaggcgccg accgcggcgt gtccgccggg
     2461 cgccaggccc ctgttggcgg ccagccagtc gggcagctca ctggacaaga aggtgtccca
     2521 ctgcttgctg ccgtcctgct cccagttggt gtacatgctg taggccccac ccgcgggcgc
     2581 caccaccgag atgcccttgc ccccgagcgt gttcatcgcg ttacccgcgg taacccagtt
     2641 gctgacgtct ggggcggcgt cgaagggatc cagcagatag accgcgtgcg gaccgccggc
     2701 caggaaggcg accggaatgt cgcggcccat cgccgccgac ggaaccatca ggctttcgta
     2761 acccgccgcc ctggccttgc cggcggctcc ggtaaccgct agcgcacaag tcagaatgcc
     2821 cacgccgacc gacagcgcgg caacacagaa tactcgcaga agcactgaca gaccccgcac
     2881 gacccgcatg tccagcctcc atcgtctagt cggtgtgttt tgcacactaa ccagcgccgc
     2941 atgacgcccg acccgcaggg tagtgaaata gaccacaccg cacaccgcga atgaacaaac
     3001 ggcggcgatc ccgagggatc gccgccgttc gttgcgttat taagcggggc tttgccggct
     3061 acgtgccgct gttatccgcg ccaccaccgg tgttaggtgt ggcacccagc gtcgcctgca
     3121 ggtctggctt catggcgttg acctgcgcac cccagtacgg ccagtcgtga gtgccgttgg
     3181 cgtcgaagtt gaacaccgcg ttgtggccgc cggccgcgtt gtaggcgtcc tggaacttca
     3241 ggttgctcgt ccgcacgaag ccctcgagga acttcgcggg caggttgtcg ccaccgagat
     3301 ccgacggctt gccgttgccg cagtacaccc agatgcgggt gttgttggcg accagcttgc
     3361 cgacctgcag cgacgggtca ttgcgctgcc atgcgccccc gtcggtcggg ccccacatgt
     3421 cgtccttctt gtagcccccg gcgtcgccca tggccagacc gatcagcgac ggtcccatac
     3481 cctgcgacgg gtccaacagg gccgacagcg aaccggcgta gatgaactga tccgggtggt
     3541 aggccgccag gatcagcgcc gacgacccgg ccatcgacag tccgaccgcg gcgctgccgg
     3601 tcggcttgac ctgcttttgg gccgacaggt actgcggcag ctcgctggtc aggaaggtct
     3661 cccacttgta ggtggtgcaa ccggccttac cgcaagcggg cttgtaccag tcggagtaga
     3721 agctggactg tccgcccacc ggcatgacaa ccgaaatgcc cgactggtag taccactcga
     3781 acgccggggt gttgatgtcc cagccgttgt agtcgtcctg cgcgcgcata ccgtcgagca
     3841 ggtagagcgc cggcgagttg gcaccaccgc tttggaattg aatcttgatg tcacggccca
     3901 tcgccgcgga gggcacctgg aggtactcca ccggcagacc cgggcgcgag aacgcgccag
     3961 cggtcgccga gccccctacg gcgccaatca gacctgatag cagggcggcg ccaacggccc
     4021 ccaccacgaa ccgccgaggc atacccgcca cggcgccgcg aaacctgtcg acaagcgtca
     4081 tccttgcttc ctcatccttt cagcgcatcc ttcatccgga gcgcatcctc gaattgggtc
     4141 gtactgcatg tgttcaccca attgctcggc tgctcctcag gccgtcccgg cccgcatcgt
     4201 cgccgcgccg agcgcagcag tgcccgtgta gtcaaccaca ggtttggtca tcccctctca
     4261 tcgagcaggt caggcaaacc gttatccggg ccaggattgc aatgcttttc aggtcgaaca
     4321 cgagcgcgca ttaccgagtt ttcgcatgtg cttggccgtc gttgtacaca ggtgagagcg
     4381 cagatgtgaa gttgctggca atgtccgccg accgcccggc ggtgaccgcc ttggggcact
     4441 tcggaaaaat tatctccgcc ggacgcctca ctcgtgggga gggggcggga tcggctgcgg
     4501 caccggcagc ccacagcggg cgagctcgta gagcgggacg cgatcgatcc ggtagccggt
     4561 gaattcgaac gaattcacca cgttggagat gaagcgatgc aagctcatcg gggcgcgcac
     4621 cgagctcagc acggcttgcg tctcggggca ttgcagtgcc gccaccgcct gggcgaccca
     4681 attctcgtcc aggtaggccg gaatcccggg atgcaccttc acccacggac cgtcggcgat
     4741 cacccagtcg gggaagaggt tcttgtcgtg cccgatccgg ccgtgcttca agcgttcggt
     4801 gtgctgcgcg agcggatttg ccaatccgat ttggtcgatc accctgacgt caagtccgac
     4861 attcatgccc accatgccca ggttggtgaa aaacaccgca tgctgcggct tttggggggc
     4921 cttgttgccc ccagaactgt ccggcggcgg ctgcaattgc ggcaccagat cccactggat
     4981 gtaattgccg gacggcaaca acaacgcccc gtcgggagtg ttgtcgagtg ccgtcagaat
     5041 cgcggccatc ctcgggtagt ccaggtagtc ctcggcggtc agggggtgcg cgtgaccggt
     5101 ggcctgcgca tagaagcggc gctcgtcgac aattccggag tagctgacgt gagtggcgtc
     5161 gtcgcccatc ccgggtgagt ttgctgccca gagcgaccac cccgcgatcc ccaaccacaa
     5221 caggcccgcc gcggcggtca cccaataacc cgtttcgcgc gaatagtcct caccgtcggg
     5281 aatcgcgacc gggatcacgg ccaccggcgc cagcaaacag aatagcggcg ccagcaacac
     5341 ccgcccatgc atgaaatcgc cgccctgccg aatccaatag agcgcctgca gcagcccact
     5401 caccaggaca aacgccacca cagccggcgg gctctgcgcc gcccgggcca gccggccgta
     5461 gccgggtgcc gccgtcgggc gcagaaacga cgggcgtcgg cgggccgcca gcaccaacac
     5521 tcccagcggc accacgagca ccagcggtat ccagaccgca tagggcgcgt tgaagttcga
     5581 gagataggtc atgccctgcg accatttatc gccggccgcg tccttggcca gggcggtgcc
     5641 gggcaccagc aggccgtagt agcccatccg gaagatttcg taggcgaccg gcagcgatcc
     5701 accggccgcg atgatcagcg cgcgacggcg ccagttgcga gccgcgacca gcatcatgat
     5761 gagggccagc ccgcccatca acgccaactc gggccgcacc aggacgctga atcccgcgac
     5821 gaaggccaac gcgccgagga acgccgggct gtccgcacgg ccccgcaccg gctgcgacca
     5881 gcgcaccatc atccaccaca gcaaccccag ataggccagc gtcaggccgc tctccaggcc
     5941 ggaggtggcg aagtcacgcg ccggcggcaa cgcgatgtag accagcgccc cggcgggcag
     6001 catgatcgcc ttgcggccgc gcaggctggg cgcatacagc cggccggtgc caagcatcag
     6061 cagcgcgaca cccagcaccg aaagcaccag agccagcgcc aacgcgacgt actccatgcg
     6121 catcgggccg ccgacccagc ttcccgcata catcaggtac gtccacgccg tggaggtgtt
     6181 cgcctccact cgctcgccct gattgaagac gggcccgttg ccggccagca gattgcgcac
     6241 cgtgcgcagc acgatcagtc cgtcgtcggc gatccagcgc ctgcgccaac tcccccacgc
     6301 gaacagcacg gcgaccgccg ccacactggt ccacaggctg atccgcacga cggtgccgta
     6361 ggggaacagc ggccgaccaa cccgcctgac caccggtcgg tggcggcgca ggaccgccac
     6421 ctcgaaggcc ttcagtctgg ggctagctga gggcaacggc ggctccaact gttgcgatcc
     6481 acgccagcgc caacagctgc agcacccggt cacgcagtgc tatgtcctcg ggctccccgg
     6541 ccagcccgcc gtcgacgtcg accgcatagc gcaggatcgc gatggtgaac gggaccatcg
     6601 acaccgcgaa ccaggatccc gaatggcgat cgcgctcgaa ggcccacagc ccgtagcaga
     6661 gcaccaccgc ggtggccgac atcgtccaga cgaaccgcag ataggtgctg gtgtagctct
     6721 ccagcgcctt gcggatcgcc gcgccggtgc gctcggcgac ctgcagctcg gcgtagcgct
     6781 tgccggccac catgaacagc gacccgaacg ccatcatcag cagaaaccat tgcgacagcg
     6841 ggatattcgc ggccacaccg ccggcgatgg cccggatcaa aaagcccgac gacacaatgc
     6901 agatgtccat caccgcttgg tgtttgaggc cgaagcagta cgccagctgc atgacgatgt
     6961 acacggccat caccaacgcc aggttgggcg tcaccgacca ggcgatcgcc agcgacgcca
     7021 cggccagcac caccgagagc gtgtaggcga gccactcggg caccacgcct gccgcgatgg
     7081 ggcggaacct cttggtcggg tgctcgcggt ctgcctcgac gtcgcgcgca tcgttgacca
     7141 ggtatatcga cgaggccgcc atgcagaaca ccacgaaggc caccgacacc ttgctcagca
     7201 cctcggcgta gtcgtagcgg accgggccgc ccaacgcggc cagcggcgcg gccagcacca
     7261 gcacgttctt cacccattgc cgcgggcgca tcgccttgat caccccggcg atcaagttcg
     7321 ccggcggccc ggtcacgacg ttttcagtgt cctcactcat tcggccccac ctctaatcga
     7381 gcccgctatc gagccggacg gcgattgccg cgacggcggc acccagggcg atgccgaaag
     7441 ccacgtcact ggggtaatgc acgcccagca ccatccgcga gattgccatc ggcggcacca
     7501 gcactgcggg caggggcagc ccggtaaccc ggcccatcag gatggccgcg gccgtggtgg
     7561 aggtggcatg cgcggacgga aagctgagct ggctgggggt gccgacattg accgcgacgg
     7621 ccgcatgatg cggccgtttg cgccgcacca gacgcttgag caggacagcg gccgcgtgtg
     7681 cggtgaatgc gccggccccg gccaccagcc agtcccggcg acgtctcggc atcaacaccg
     7741 caccaagcag ggacaccgcc acccaaccga tgctgtgctc gccgaagtgc gacagcacgc
     7801 gtgcgacggg caacactcct ggccggtcgg tgagcgccga ctgaacggcg accagcgcgg
     7861 ccacttcgcc gcggggtgtg tctgcgggaa gggattcagg catgctcggg cttgtccgcg
     7921 gcaggctggg gatcccaccc gcttgcgggg gactttgcca gtttcggggc cggtttcggg
     7981 gccgggttca gggccgtctc ccacttctgc ttactggaca acacgggcag cgcgtcccgg
     8041 tacagcttgc gcatctcgtc gaaccggctc gccagctgac gctgccggcg caacgactgc
     8101 aacagcagcg agaacatctt gcgccgatcg cgctgccggt agaccacgcc gcacccgtcc
     8161 gcggtggtga cggtgacacc gtcgaccgtg cacaaccgga accagcgggc gtcctgggtc
     8221 ggcacgttgt actccgggcg ctggtgggcg tcggggtcgg cgggtgtcat gttgtgcacg
     8281 atcccgcggg ccaatcggta gccgatggac aacgggttca ccggcggctt catcgccttg
     8341 gtcttgtgca gcggcgacgg cagttcgctc gccgccggca gtacgaccgc gtccgggtac
     8401 tccttgcgca tgcggtgcac ttccggcagc gcgctttcca gaatcgagaa gatgtgctcg
     8461 ggacccgcaa ggaagtcgtc gatggcccgg ttctggatcg cgaccgtcga atattcgagg
     8521 caggcaaggt gtttcagtgt cgccttgaga tggctgcgga ccagtccggt gacgtcgccg
     8581 tcccagtgca gggccgccac caccagccgg ttgcgcaaat ggaagtacgc ctgccagtcg
     8641 atcgcgtcgt ccttgtcgct ccaggccata tgccagatcg ccgcgccggg caaggtgacc
     8701 gtcgggtagc cgtgctcccc ggcgcgcaga ccgtattcgg cgtcgtccca cttgatgaac
     8761 agcggcagcg gctggcccat ctcctcggcg acttgccgcg ggatcatgca cgtccaccag
     8821 ccgttgaagt cgacgtcgat gcgccggtgc aacagcttgc tgcgagcgtt gtcgtcgttc
     8881 agcgggtatt cggcgaaatt gtggtcgtac tccgcgtgcg ccgcggcggt ccacatgaag
     8941 ttcgacgggt tgacgacctc gcccatgatg tgcaggtgcg acggctcctg caggttgagc
     9001 atctgaccgc cgatcagcat cggggtcttg gcgaagcggt gcatcgccag cacccgcagg
     9061 atcgagtcgg gctcgatgcg gatgtcgtcg tccatgaaca gaatctgttg gcagtcggtg
     9121 tttttcagcg cctcgtacat cacgcggctg taaccgccgg agccacccag gttgggctgg
     9181 tcgtgaatgg agagccgatt tcccagccgg gcggccgcgg ccgggaagtc cggatggtcg
     9241 cgcaccttgc ggacaccctg gtcgggcacg atgaccgcgc cgatcacctt gtccaccaag
     9301 tgatccgcgg ttagctcgcg cagagcgttg acgcagtcgt tcggccggtt gaaggtcggg
     9361 atgccgacgg cgatgttggc cgtgccggga gcgggctcgg ccgcatacca gccgccgctg
     9421 accagcgtga ccttgctgtc ggtggtgatg tcgaaccaga tccagccacc gtcctcgaaa
     9481 ggcttgagcg gcacctcgat ctcgaggacc tgcggctcgt cctcgctgcc ggcgaattgg
     9541 tggccctcga tgaagattcg cgcaccggtg gccttggtgc ggtagaggtc gacccgaccg
     9601 gcgccgctca cctcgacccg cagcaccacc gattgacaga tcgaccaccg gcgccagtag
     9661 ctggcgggaa aggcgttgaa ataggtcgcg aacgacacct cggactccgc accgatctgc
     9721 agtgaggtgc ggctcgtcgc gtgcgcgcgc cgggcgttgg tggacgactc ctcgaggtac
     9781 agcttgcgca cgtcgagggg ttcacccgga cgcggcagga tgatccgggc cagcaggctg
     9841 acggcggtca ttccagtgct ccctcttcag ctaacggagc tccgtcctgc aggtgcggcg
     9901 cgaggacgtt gtcgtacatg ctcaacgcgc tggcaatggc catgtgcata tccagatatt
     9961 ggtaggtgcc cagccggccg ccgaacagta ctttcgatga cgcggtctcg gacttcgccc
    10021 tggctcgata ggcggccaac agggcgcggt cggcctcggt gttgatcgga tagtagggct
    10081 cgtcgtcgtg ttcggcgaag cgggagtact cgcgcatgat caccgtcttg tcggtcggat
    10141 agtcgcgctc aaggtggaag tgccggaact cgtggatgcg cgtgtagggc acgtcgagat
    10201 cgttgtagtt catcaccggc gtgccctgga aatccccgat cggcaacact tccacctcga
    10261 agtccagggt gcgccagcct aaccggccgt cggcgtagtc gaagtagcgg tccagcggac
    10321 cggtgtacac aaccggagcg tccggattgt ccgcgcggag ttggtcacgg acgtcgaacc
    10381 agtcggtgtt cagcctgacc tcgatgcggt cgtcggcggc catgttcgtc agccatgccg
    10441 tgtagccatc ctgcggcagg ccctcgtaag tgtcgctgaa gtaccggttg tcgaaggtgt
    10501 agcgcaccgg cagcctgttg atgacggcag ccgggagctt ggcgggatcg gtctgccact
    10561 gcttggcggt atagcccttg acgaacgcct cgtagagcgg ccggccgatc agcgagatgg
    10621 ccttctcctc gaggttctgg gcgtccgcgg tgtcgatttc ggccgcttgt tcggcgatca
    10681 gctgccgcgc ttcgtcgggg gtgaagtact tgccgaagaa ctgcgacacc aggcccaggc
    10741 ccatcgggaa ctggtaggcc tggccgttgt gcatcgcgaa gacgcggtgc cggtagtcgg
    10801 tgaactcggt gaattgccgc acgtagtccc aaaccctctt gttggaggtg tggaacaggt
    10861 gggcgccgta cttgtgcacc tcgataccgg tctgcggctc ggcctccgaa taggcgttgc
    10921 caccgatgtg cgggcgccga tcgacgacga gcacgcgctt cccgagttgg ttagccacgc
    10981 gctcggcaat ggtcaggcca aagaatccgg agccgacgac gaagaggtcg aagcgagcag
    11041 tcatcggttg cttagggtat ctgaccgagc gggcctaacc catgtggcga ggccacgcaa
    11101 tcggagttcg gcgaagtaat ggcggcctgg gcggcctcaa atccccgatc acagccggag
    11161 tcgcaattac ctcacgattc aatatcagca ctctagtcac accaatccca ctcgtaccat
    11221 cgactttgtg tgcttcatgc cggataggac gggcagggca cgccgacaca acacgcagtt
    11281 caaatagttg tccagatatg aggagacttc cgtgccgaac cgacgccgac gcaagctttc
    11341 gacagccatg agcgcggtcg ccgccctggc agtggcgagt ccgtgcgcat acttccttgt
    11401 ctacgaatcg accgcaggca gcaagccggc cgagcaccac gaattcaaac gtgcggcggt
    11461 gatgaccgac ctgcccaacg agctgatgtc cgcgctgtcc caggggctgt cgcagttcgg
    11521 gatcaacttg ccgtcggtgc ccgccttgac cggcggcgcc agcacgacaa gtctgaccag
    11581 cccagggttg ggaagtcctg gcctcaccag cccgggcctc accagccccg gtctgaccag
    11641 cccgggcctc accagtccag gcctgaccag tccgggtctg accagtccgg gtctgacgcc
    11701 cggggcgacc gggctcaccg cgcccggcgc gctgacgacc acgcccggcg tgggagggct
    11761 ggccaatcct gcgctgtcca accccggcct gaccagcccg gccggactca cacccggcct
    11821 gggtgctgcc aacgaagtgc cgatcagcgc gccgaccggg ttggacccgg gcgccgacgg
    11881 cacctatccg atcctcggtg acccgtcgac gttgggcgga gggccgctcg ggggcggctc
    11941 cgggggtggc tccggcggtg gcgggctcgt caacgacctg atgcaagccg cgaaccagtt
    12001 gggcgccagc caggccatcg acctgctcaa gggcctggtc atgccggcga tcatgcaggg
    12061 tgtccagcaa ggcggcgccc cgggtgccgc cggcgccctg cccggtgctg ccggtgccct
    12121 gccgggtgcc gcgggtgccc tgcccggtgc cgcgggcgcc ctgcccggcg ctgccggcgc
    12181 gctgccgggt gccgcgggtg ccctgcccgg tgccgcgggt gccctgcccg gcgttgccgg
    12241 cgctgcggcg cctgccgcgg cgggtgccct gccgggtggc gccaccagcg ccctgacggg
    12301 cgccgccaca ccggtgaagt tgccgttgcc ccactagagc tccgaggaac ctgtcacctg
    12361 acggcaccgc agagtcttgt ctggccctgg actagcaacc cgcccggcaa ggacacctct
    12421 gcggtgccgt cgaacaggct ttcgtggact tttccggcac gcacgtcgtg tcgcctcagg
    12481 aataacacca gaaacacacg taacatcagc tggtgtcgtc ccgccatcgc gcgccaacga
    12541 tgttgctcac cgccatcgcg gcgaccgtcg ttatcgtctc gtgggtgggc gacgcggtcc
    12601 gcgatcccgg cgcgcccgac ccgccgcggg cgcgtggcac ccaactcacc gagcagccgc
    12661 tgaccgggct cggagcagga gtcaccgtcc gtgaagtaag ccaggacacc ccgttttcgt
    12721 tggtggcgct gaccggcgac ctcgccggca cgtccacccg ggtgcgcgcc aagcgcccgg
    12781 acggttcctg ggggccctgg tatcggaccg agtacgaaac cgcggcaccc gacgccagcc
    12841 cagcagccgg cacagggccg gccggggccg tccggccggc gggatcgagc gagggtcccc
    12901 gcagcacaga cccggtgttc gtcggcacca ccaggaccgt ccagatcgct atcacccgcc
    12961 ccctcgatgc gccggtgacc ctgggggcag cgccggcaag cgtcccgaca ccgcaggacg
    13021 ggctgggcta caagccgatg tccaaggaac agccctacgc gcagaacatc tccgcgatcc
    13081 tcatctcccc gccgcaggcc ccggccaacg cgcagtggac accgccgtcc ggagtgctga
    13141 tgcccggcca gccgcccagc atcatcagcc gggcggaatg gggcgccgac gagtcgctgc
    13201 gctgcggtag cccgcagtac gacaacggga ttcgcgccgc ggtcgtccac cacaccgcgg
    13261 gcagcaacga ctactcgcca ctggagtcgg cggcgatcgt caaggccatt tacacgtacc
    13321 acagcaagac gctgggctgg tgcgacatcg cctacaacgc gctggtggac aaatacggcc
    13381 aggtcttcga gggcagctcc ggtgggctga ccaaagcggt cgagggcttc cacaccggcg
    13441 ggttcaaccg caacacctgg ggtgtggcga tgatcggcaa cttcgacgac gtgccgccga
    13501 cgccgatcca gctgcgcacg gtgggccggc tgctcggctg gcggctgggc ctggacggcg
    13561 tcgaccccaa gggcacggtg gcgctggagt cggcgggcag tcactacacc acctacccgg
    13621 ccggcgccat cgcgaacctg cccgccatct tcacccatcg cgacgtcggc aacaccgact
    13681 gcccgggcaa cgccgcctac gcgctgatgg acgaaatccg cgatatcgca tcgcatttca
    13741 acgatccgcc cgaggagctg atcaaggcgt tggagggcgg cgccatctac gagcactggc
    13801 aggcgatcgg cggcatgaac agtgtcctgg gcgcgccgac gtcgccggaa gacgacgccg
    13861 acggcgatgc ccgctacgcc accttcgcca agggcgcgat gtattggtcg cccgagaccg
    13921 gcccccagcc ggtcaccggt gcgatctacg acgcgtgggc ctccctgagc tatgaacgtg
    13981 gtccgctcgg cttgccgacc agcgcggaac tacaagagcc gctgcagatc acacagaact
    14041 tccagcatgg aacgctgaac tacgagcggc tcaccggaaa cgtcaccgaa gtcgtcgacg
    14101 ggatcacgac gccgctgtcg acgcaatccc cgagcggccc gacggtgccg cccgagcact
    14161 tctcgctgcc gagccatccg accgtcacgt aactgcctgc cagccgtggc acggtcgacg
    14221 ccgaatcgct tgtagcgtcg caccttccac cgcaggcgcc cacggcggtc ggccacgtca
    14281 gtgtgtgcgc atgcacggga gtagaacgcc ggttttgggc cgacgtgcgc tgcgtcacag
    14341 taggctcgac ggtggccccc ggcagccagg ggtcgcgggg ggactggtta cgaggggaga
    14401 gggccgtgtc gttcgtggtc accgcgccgg agctgatgac ggccgcggcg gggaatcttg
    14461 cgggcatcgg ctcgaccctg gaagaggcga cctcggcggc agccggcccc acgaccggag
    14521 tcgcatccgc ggccgccgac gaggtgtcgg tcgcggtctc gcagctgttc ggcacctacg
    14581 cccaggaatt tcaaacggtc agcgggcagg cggcaacgtt ccacaacgag ttcgtgagtt
    14641 tgttggcggg cggtggggcg gcatatctca gcgccgacat cgccaacgcc gagcagagcc
    14701 tgcttgggca gctcccgagc gccggcgtcg tgtccgcgct ggcgaatggc ggggctgcct
    14761 cgctgctctc cgacaggatc gcgacgggtg cgcaagccgc ttccgcggcc atcgcaggca
    14821 cgcccgcact gcgcgggctc gagacggcct tgctgcccgg gctgtttgcg ccgggcgcgg
    14881 ccgccgctac cggtcccggc ggcgcctatc ggcaactcct tgccaacacg ggcaccaatc
    14941 tggaatccct ctttggtacc tggagcgctc acccgttccc gctgctgaat cagatcgcgg
    15001 ccaatcaacg gggctactgg ttggagatcg ccacagcact cgccggtggc attcagaacc
    15061 tccccgcgga attggcgaac ctgccacaga cgatccaggc cggcgttcag ggactcttgg
    15121 ccttcaatgc ggcgttttac gtacagcaat acatcaccac gcagatcggc ttcgcccaga
    15181 ctttcgtcac gaacctagac ggcgcggtaa ccggcatcgt ggacggcctg cccaattttg
    15241 tcgcgggcct acagaccgcc cttcacgccg tccgagcggg cgattacttc ggcgcggtgc
    15301 aggacgtggc gcaggcgtac gccaatctct tggttacggg gctcaatccc ggcaacctga
    15361 cggttgactt gctgactccg ctcgtgaatc ccaccgtggt tgcgacgacc aatcccacac
    15421 ttcttggccc gctggggaat ctgttcgcca tcgcgaacat tcccggacaa gaggcgcagt
    15481 acttcaccga cctgatgcct ccgtcgatcc tccgacagat gtcgcagaac ttcaccaacg
    15541 tgctcgatgc gctcacgatt cccagcattt cggcgaccgc gacgttaccg ctgctgaatc
    15601 ccacagcagg atcgctcagc gcctccttcg ggttgccgtt ggtgctcacg tatgcggtgg
    15661 cgggtgcgcc ggtcgccacg ctcaacgggg tcgcaaccag cgcgacggca gttcagcagg
    15721 ccctgttggc cggcgacggt ctaggcgcgt tcaatgcgtt cgtcgatgca ccagccgtca
    15781 tcgcaaacag cttcctcaac ggcgaggtcc tcgtagattt cacggtgccg gtgccggtga
    15841 atctccccgc acccctcccc agcttcaccg ttccgatcat cctgcatttg ccctttgacg
    15901 gcattcttgt tccgccgcac cccgttacgg cgacgataga cctgaacggt tttgtccccg
    15961 gtttagaaat tccggcaacc atcttcggta cacccttcat gggcctcgtt ccgctgttgg
    16021 tcaactacgt gccccaacag ctggccgcgg caatcacacc cgcggcgtag ccgcttcacg
    16081 gcgcgcatcg tcgtcgggct caaaccaacc gcccgactcg ggctagagcc accagcgttc
    16141 cagcacccgc cccacgccgt cgtcgctgtt gggcgtggtg acctcatcgg cggccgccag
    16201 cacatcggga tgtgcgtttc ccatggccac gccatgaccc gcccacagca gcatcgggat
    16261 gtcgttgggc atgtcgccga aggccaccac gtcggcgctg gtgatcccca gcggtccggc
    16321 gatctctgcc acgccgctgg ccttgctgat ccccagcggc acgatctcca ccagcccgtt
    16381 gttggtggag taggtgatat cgccctcgat gcccacatgc ttggccagtt cggcggccat
    16441 gtccgcgctg cgggcgccgg tcttgcggat caacagtttg atcgccggcg cgctgagcag
    16501 gtcgtcgatg gacacctcag tgttgtccgg gttgagccaa gcgtgctcgt agcccggtga
    16561 gctgatgaat tggggagttg ccgtgtcgtg ggcgcggtcg ccgatccgct cgacggccag
    16621 gcccgccccc gggatgacgc gggatgcgag ttcggccaac tcggcgagcg tgtcgacgcc
    16681 cagcgtgcgc accgacacca ctcggtcgtt cgcggggtcg tagatcacgg cgccgttggc
    16741 gcacaccgcc gtcggcgcga acccgagtgc ctcgacgacg ggacgtatcc agcgcggcgg
    16801 gcgtccggtc gccaccacga actgcgcacc ggcggcgacc gcagcacgca cggcggcgcg
    16861 ggtgcgcggg gtgatggatt cgctttcgtc gaacaacgtg ccgtcgacgt cgcacgcgat
    16921 gagcgcaggc aaagtcatcg agacgatccg cccgcggtcg tcaccgcgaa ccgcgtgggg
    16981 tctgccccgg ccgcgtcacg ccgtcggtcc cgtacttctc aacccgctcg accaattcgg
    17041 cgacccggag cgcccgggag tcatcctgcg tcggcgcgct accaccgagt cgctccggga
    17101 cccagtactc gccttcagga tgcgggtact cggcctgcac ccggtagagc atcgcattca
    17161 tcgcctgccg cagagtggcg ttcaactgct cgggggtgcc ctccggcggc aacgggcgcc
    17221 gggcggcaac ggtgatggga accttgttgc ggaacaactt ctttggatga tccttcggcc
    17281 aaatccgctg tacaccccag acgatcatgg ggatgatcgg cacttcggct tccaccgcca
    17341 tcctggcggc cccggtcttg aattcccgga gctcgtaact gcggctgatc gtggcctccg
    17401 gatgcatccc gatgagttcg cccgctcgca gccgctgcac cgcaacctcc aaagcgtcgt
    17461 gccccttcct gcggtccacc gggatcagcc gggcgtgctt gatcacgtag ttgaccgcct
    17521 tcacatccgc catttcggct ttgatcatga agtacctgcg ccggtggcgc tcgcggacgg
    17581 cgaacatcga cggaagccaa tcgaggtagc tggtgtggtt ctgggccagt agggccccgc
    17641 cctgcacggg aatgttctcc aggccctgga acgtgaattt cgttccgttc atcgccacga
    17701 ccggcggaat gacccactcg cacagccggt aaaacggctc tgccatgttc tctccttaca
    17761 cccctagcgg gactggcgcg gggttcggcc ggcggctctc gccgccagct catcggcatc
    17821 catccgggcc gcctcggcca acgtgggcgc cccgccgccg agccggcgcg gaacccaata
    17881 cgccccggcg gggtgcggat accgccgttg cgtctggtgc agcagcgtgg ccatcgactc
    17941 gcgcaacgcg gcgtcggtgg ccgcgacgtc cttggtggcg cacaaaggcg cacccacctg
    18001 cacggtgatc ggtattctgt tgcggcccag gtgccgtggg tggtctttgg tccagatccg
    18061 ctgcgcaccc caaacgatca ccggaacgat cgggacgttt ccttcgatcg ccatccgggc
    18121 ggcacccgtc ttgaattcct tgagttcgaa gctgcggctg atcgtggcct ccggatagac
    18181 cccgacgagt tcaccctcgc gtagtcgctg caccgcgacc gcataggcct ccgccccggc
    18241 gcagcggtcc accgcgatgg ttccggtgtg attgatcagg aaattgacca ccttcacctg
    18301 ttgcatctcg gccttgatca tgaatctaag ccgacggtgc cgccgataca cggccaacgc
    18361 ggcgggcaga aagtcgacgt aactggtgtg gttgatcgcg atcacggcgg cgccccggtc
    18421 ggggatgttc tcctcaccgc ggtaggtgat gcggctgcca gaggccagga cgccgagtcg
    18481 ggccacaatc tcgatcgcgc gaaaggttgg ctccggcatc gcctactgct ccggcggccc
    18541 agtggcttgg gcgcggcgag ccgccctggc ggccgcttcc tcggcgtcca tgcgggtcgc
    18601 ctcagccaac gacggagcgc caccgcccag ccgacgcggc acccagaact cacctgccgg
    18661 atgcggtcca tacatttcct gggcccgttc cagcaggtgt tgcatccgcg agtgcaacag
    18721 cgacttcaat tcctcgacgc ccagcgtcgg ttcgatcggt tcgccgacca gcaccgtgat
    18781 cggcaccttg ggacgccaca gctttttggg gtggcctttg gtccagatcc gctgagcgcc
    18841 ccagacgatg tggggaacga tcggcactcc ggcctcggtc gccatgcggg cggcccccga
    18901 cttgaactcc ttgatctcga agctgcggct gatcgtcgct tcggggtaga cgccgaccag
    18961 ctcaccgtct ttcaggtgcc tgacggccgc ctcgtaggac gcggcaccgt tctgccgatc
    19021 caccggtatg tgccggacgc tgcgcatgag cggcccggtg atcttgctgt cgaacacttc
    19081 ctgcttggcc atgaaccgca ccttgcgacc caggccctgc tggtaggcgg gcaaaccggc
    19141 gaacgtgaag tcgaggtagc cggtgtggtt gatcgcgatg accgcgccgc cactggtcgg
    19201 taggttctcg acgcccgtga ccgtgatcgt gagaccctgg acgcgccaga gcaaccgaac
    19261 aagctgaatg acagtcgcgt ataccggttc cacagactgt cagcctagtg ctcccgacac
    19321 taagtcgccg gaagtggtga aagagggcaa cgcttgcggc aggccgacga cgtggcctcg
    19381 cgctaagctc gggggctgac aagccgtcac catttcgagg ctcgtaccat tccgagaaag
    19441 gcatcagtgc aggtcaccag cgtaggccac gccggatttc tgatccagac ccatgcgggc
    19501 aacatcctct gcgacccgtg ggtcaacccc gcgtacttcg cgtcgtggtt cccgttccca
    19561 gacaacagca cgctggactg gg
//
LOCUS       DASSGQ010000270         5203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1204123,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000270 DASSGQ010000000
VERSION     DASSGQ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204123"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1206)
                     /locus_tag="VFI55_11935"
     CDS             complement(184..1206)
                     /locus_tag="VFI55_11935"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HET7074777.1"
                     /translation="MIVVLFDRRRPSLVPVEAIEHLAGEVQYTEEMPVAVPWSLPAAR
                     PVHVGDDAPVLLSSDPDHPAVTLRLAAGARLISAPETPRGERLVDAVAMMDKLRTAGP
                     WESEQTHDSLRRFLLEETYELLDAVRIGNADQLREELGDVLLQVLFHARIAEDAAQFP
                     FTIDDVAVTLMRKLGNRVPGVLAGRPISLEEQLAQWEVRKAAEKPRNSVMDDVHTGQP
                     ALALAQKVIERATKAGLPADLIPGEITAVSVSADTDAESALRTAVLEFVDTVRRAERA
                     IGAARRGDSVTTELDVAPLGEVTEEEWREHWSAGEPAAIDAAVSDSGVKSKAPGKGSK
                     RPRGRR"
     gene            complement(1203..4925)
                     /gene="mfd"
                     /locus_tag="VFI55_11940"
     CDS             complement(1203..4925)
                     /gene="mfd"
                     /locus_tag="VFI55_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET7074778.1"
                     /translation="MTAPGPARPETPIAGLAELALTAPTFQQLIESAAARPDELSLVG
                     PASARLFVASALAQQGSLLVVTATGREADDLAAELRGVFGTAVAVFPSWETLPHERLS
                     PGVDTVGTRLMVLRRLAYPDDARLGPPLRVVVTAARSLLQPMAPRLGHQEPVTLRVGQ
                     EIGFEDVIARLVELAYTRVDMVGRRGEFAVRGGILDIFAPTAEHPVRVEFWGDEVSEM
                     RMFSVADQRSIPEIEVETLVAVACRELLLTADVRARAAELVARQAAGRPRGEAAISGS
                     VSDMLAKLAEGIPVDGMEALLPVLKPDDHALLVEQLAEGTPVLLCDPEKVRTRAADLI
                     KTGREFLEASWSVAAMGTDAPVDVEQLGGSGFAELDDVQAAAHRSGHPWWTLSQLSDE
                     SAVELDVKAAPSARGRQSAFGDIEAIFAMLRAHVLTGGYGVIVAPGTGTAHRVVERLA
                     ESDTPAAMLDAGAAPKSGVVGVLKGPLHGGVVIPGANLVVITEADLTGSRATSVEGKR
                     LAAKRRNTVDPLALNAGDLVVHDQHGIGRFVEMVERTVGGARREYLVLEYASSKKGSG
                     TQKSTDKLYVPMDSLDQLSRYVGGQAPALSRLGGSDWVNTKTKARRAVREIAGELVSL
                     YAKRQASAGHAFGPDTPWQAEMEDAFGFTETVDQLTAITEVKSDMEKPIPMDRVICGD
                     VGYGKTEIAVRAAFKAVQDGKQVAVLVPTTLLADQHLQTFTDRMAGFPVIVKGLSRFT
                     DTTESRAVIDGLADGSVDIVIGTHRLLQTGVHWKDLGLVVVDEEQRFGVEHKEHIKSL
                     RTHVDVLTMSATPIPRTLEMSLAGIREMSTILTPPEERYPVLTYVGPQEEKQIAAALR
                     RELLRDGQAFYVHNRVSSIDRSAARVRELVPEARVVVAHGQMPEDLLERTVEGFWNRE
                     HDILVCTTIVETGLDISNANTLIVERADTFGLSQLHQLRGRVGRSRERGYAYFLYPPE
                     VPLTETAYDRLATIAQNNELGAGMAVALKDLEIRGAGNVLGVEQSGHVAGVGFDLYVR
                     LVGEAVEAYKAAADGETVTTAEEPKDVRIDLPVDAHLPPDYIASDRLRLEAYRRLAAA
                     ASDREVGAVVEELVDRYGALPEPAQRLVAVARLRLICRSAGVTEVSAPSAATVRLSPI
                     TLPDSAQVRLKRMYPAANYRATTSTVQVPIPRAGGASGGLGAPRIRDVELLQLVANLV
                     TALQGKPQQDVGITSSSPDGDKDSQPAAMSTEERRT"
     gene            5045..>5203
                     /locus_tag="VFI55_11945"
     CDS             5045..>5203
                     /locus_tag="VFI55_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET7074779.1"
                     /translation="MTLLDILPSIGKAAPRRLDPTIWPATTHSDEEGRLCVGGVTLAD
                     IADEFHTPT"
BASE COUNT          787 a         1855 c         1729 g          832 t
ORIGIN      
        1 attccgccga attagcgatt agctgccggc accgatggct agcaaagcgc gaagaacgac
       61 gaccgctcgg gttcgcaact cacacgcgat atcaccgggc cgttggcgag caaagtggca
      121 aacatcacgg tgatcgctgt ctcggcggcg cccggcacag cggtggcccg gatcaaaccc
      181 gggctaccgc cgcccccggg gccgcttgga accctttccg ggcgccttgg acttaacacc
      241 ggaatcgctg accgccgcgt cgatcgctgc cggttcaccg gccgaccagt gctcgcgcca
      301 ctcctcttcg gtgacttcgc ccagcggggc tacatcgagc tccgttgtca cgctgtcacc
      361 gcggcgcgcg gcgccgatcg cccgctccgc ccggcggacc gtgtccacga attccaaaac
      421 cgcggtccgc agcgcgcttt ccgcatcggt atcggccgac accgacaccg ccgtgatctc
      481 gcctgggatc aggtcggcgg gcagccccgc tttcgtggca cgctcaatca ccttctgcgc
      541 cagagccagt gcaggttgac ccgtgtggac gtcgtccatc accgagttgc gcggcttttc
      601 ggcggccttg cgcacttccc actgggccag ttgttcttcc aacgaaatcg gtcgtcccgc
      661 aagcactccc ggtacccgat tgcccagttt tcgcatcagc gtgacggcga cgtcgtcgat
      721 ggtgaaagga aactgcgctg cgtcctcggc gatgcgggcg tggaacagca cctgcagcag
      781 cacgtcaccg agttcttcgc gcagctggtc ggcgttgccg atgcggaccg cgtccagcag
      841 ctcgtaagtc tcctcgagca ggaacctgcg caacgagtcg tgggtttgct cgctttccca
      901 cggtcccgcg gtgcgcagtt tgtccatcat cgcgacggcg tcgaccagcc gttcgccgcg
      961 cggcgtttcc ggcgccgaga tcagccgggc gccggccgcc aatcgaaggg tgacggccgg
     1021 gtggtcggga tcagaggaca gcaacacggg tgcgtcgtcg ccgacatgca ccgggcgcgc
     1081 ggcgggcagc gaccagggca ccgcgacggg catctcctcg gtgtattgca cctcaccggc
     1141 gaggtgctcg atggcttcga cgggcaccag cgatgggcgg cggcggtcga acaagaccac
     1201 gatcatgtgc gccgttcctc tgttgacatc gctgcaggct gggaatcctt atcgccgtcg
     1261 ggcgacgaac tcgttatacc aacatcttgc tgtggcttgc cctgcagtgc tgtcaccagg
     1321 ttggccacca gttggagcaa ctcgacgtcg cggatgcgtg gcgcgcccag cccacccgag
     1381 gcgcccccgg cccgcggaat ggggacctgc accgtggagg tggtggcgcg gtagttggcg
     1441 gccggataca tccgcttgag ccgcacctgg gcagagtccg gcagggttat cggcgacagc
     1501 cgcaccgtcg cggcggacgg cgcggacacc tcggtgacgc cggcgctgcg gcagatcagc
     1561 cgcagccgcg ccacggcaac caaccgctgg gcgggttcgg gcagcgcccc gtaacggtcg
     1621 acgagctctt ccacgaccgc gccgacctcg cgatcagaag ccgccgcggc cagccggcgg
     1681 taggcctcca ggcggagccg gtcgctggcg atgtagtccg ggggtaggtg cgcgtccacc
     1741 ggcaggtcga tccgcacgtc tttgggctcc tcggcggtgg tgaccgtttc gccgtcggcg
     1801 gccgccttgt acgcctcaac ggcctcgccc acgagccgga cgtacaggtc gaacccgacg
     1861 ccggcgacgt gacccgactg ctcgacaccc agcacgttgc cggcgccgcg gatctcgagg
     1921 tccttcaagg cgaccgccat gcccgcgccc agctcgttgt tctgcgcgat ggtcgccaat
     1981 cggtcgtagg ccgtctcggt cagcggcact tccggcgggt agaggaagta ggcatagccg
     2041 cgctcgcggc tgcggccgac ccgcccccgc agctggtgca gctgggagag gccgaaggtg
     2101 tcggcgcgct cgacgatcag cgtgttcgcg ttggagatgt ccagcccggt ctccacgatc
     2161 gtggtgcaga ccaggatgtc gtgctcgcgg ttccagaacc cctcgacggt gcgttccagc
     2221 aggtcttcgg gcatctggcc gtgcgcgacc acgacccgcg cctcgggcac cagctcgcgt
     2281 acccgggcgg cggaccggtc gatcgagctg acccggttgt gcacgtagaa ggcctggccg
     2341 tcgcgcagca gctcgcggcg cagcgcggcg gcgatctgct tttcctcctg cgggccgacg
     2401 taggtcagca ccggatagcg ctcttcgggc ggcgtcagga tcgtcgacat ctcccggatg
     2461 ccggccaggc tcatctccag ggtgcggggg atcggggtgg cgctcatggt cagcacgtcg
     2521 acgtgggtgc gcagcgactt gatgtgctcc ttgtgctcga cgccgaaccg ctgttcctcg
     2581 tcgaccacca ccaggcccag atccttccag tgcaccccgg tctgcagcag ccggtgggtg
     2641 ccgatgacga tatctaccga cccgtcggcc aggccgtcga tcaccgcgcg ggactccgtg
     2701 gtgtcggtga accgggacag gcccttgacg atcaccggga acccggccat ccggtcggtg
     2761 aacgtctgca ggtgctggtc ggccagcaac gtggtgggga ccagcacggc gacctgcttg
     2821 ccgtcctgca ccgccttgaa cgccgcacgc accgcgatct cggttttgcc gtagccgacg
     2881 tcgccgcaga tcacccggtc catcgggatc ggcttttcca tgtccgactt gacctcggtg
     2941 atcgcggtga gctggtcgac ggtctcggtg aacccgaacg cgtcctccat ctcggcctgc
     3001 cacggcgtgt ccggcccgaa cgcgtggccc gcgctggcct gccgtttggc gtacagcgac
     3061 accagctcgc cggcgatctc gcgcaccgcg cggcgggcct tggtcttggt gttgacccag
     3121 tcgctgccgc ccagcctgct cagcgccggg gcctggccgc cgacataccg cgacagctga
     3181 tccagcgaat ccatggggac ataaagcttg tccgtgctct tttgagtgcc agaccccttc
     3241 ttgctggagg cgtattccag caccaggtac tcgcgccggg cgccgccgac ggtgcgctcg
     3301 accatctcca cgaatcggcc gataccgtgc tgatcgtgca cgaccaggtc gccggcgttc
     3361 agagccagcg ggtcgacggt gttgcgccgc ttggccgcca gccgcttgcc ctcgaccgac
     3421 gtggcgcggc tgccggtcag gtcggcttcg gtgatgacga ccaggttggc gccgggtatg
     3481 accacgccgc cgtgcagcgg acccttgagc accccgacga caccgctctt gggagcggcg
     3541 ccggcgtcca gcatggcggc cggcgtgtcg gactcggcca gccgctccac gacgcggtgc
     3601 gcggtcccgg tgcccggcgc gacgatcacg ccgtacccgc cggtcaacac atgggcgcgc
     3661 agcatcgcga agatggcttc aatatcgccg aaggcgctct gccgcccgcg cgccgacggc
     3721 gccgccttga cgtccagctc caccgccgac tcgtcggaca actggctcaa cgtccaccac
     3781 ggatgcccgg accggtgcgc ggcggcctgc acgtcgtcca attccgcgaa gcccgacccg
     3841 cccagctgct cgacatcgac cggagcatca gtccccatcg cggccaccga ccaggacgcc
     3901 tccagaaatt cacggccggt cttgatcaga tcggcggccc tggtacgcac cttttccggg
     3961 tcgcacaaca acaccggcgt cccctcggcc agctgttcga ccagcagcgc gtggtcgtct
     4021 ggcttcagga cgggcagcag cgcctccatg ccgtcgaccg ggatgccttc ggccagcttg
     4081 gccagcatgt ccgagacgct gccgctgatc gcggcttccc ctctcggccg ccctgccgcc
     4141 tgccgtgcga ccagctcggc cgcccgcgcc cgcacgtcgg cggtgagcag caactcgcgg
     4201 caggcgacgg cgaccagcgt ttcgacctcg atctcgggga tggagcgctg atcggcgacg
     4261 gagaacatcc gcatttcgct gacctcgtcg ccccagaact cgacgcgcac cgggtgctcg
     4321 gcggtcgggg cgaagatgtc gagaatcccg ccgcggacgg cgaactcgcc gcggcggccg
     4381 accatgtcca cccgggtgta tgccagctcg accagccggg caatcacgtc ctcgaaaccg
     4441 atctcctgac cgacgcgcag ggtgaccggt tcctgatgcc ccagccgtgg cgccatgggc
     4501 tgcaacagcg atcgcgccgc cgtcaccacc acgcgcaacg gcgggcccag ccgggcgtca
     4561 tcggggtagg ccagccggcg cagcaccatc aggcgggtgc ccacggtgtc gacgccgggc
     4621 gagagccgct cgtgcggcag cgtctcccag gacgggaaga cggccaccgc agtgccgaag
     4681 acgccgcgca gctcggcggc aaggtcgtcg gcctcgcgcc ccgtcgcggt gaccaccagc
     4741 agcgaaccct gctgcgccag cgcgctggcc acgaagagcc gggcgctggc cgggccgacc
     4801 aggctcaact cgtcgggtcg agcggccgcg ctctcgatca actgctggaa agtcggcgcc
     4861 gtcagcgcca attcagcgag ccccgcgatc ggggtttctg ggcgagcagg ccccggtgcg
     4921 gtcatgatga ggccattcta agtgcctatc tgtgagcgcc gaacttcgtc aatattgagc
     4981 ccggcgccgc taagggatcg taaggaatgc ggcgcgtgac gccgtgcgag cgcaatttga
     5041 agtcatgaca ttgctggaca tcctgccgtc tattggtaag gcggcacccc ggcggttgga
     5101 tcccacgatc tggcccgcca ctacgcattc cgacgaggag ggccgactct gcgtcggcgg
     5161 cgtaacgctg gctgatatcg ccgacgagtt ccacaccccg acc
//
LOCUS       DASSGQ010000271        10174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1051978,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000271 DASSGQ010000000
VERSION     DASSGQ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10174
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051978"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /gene="fadD3"
                     /locus_tag="VFI55_11950"
     CDS             <1..916
                     /gene="fadD3"
                     /locus_tag="VFI55_11950"
                     /EC_number="6.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-((3aS,4S,7aS)-7a-methyl-1,
                     5-dioxo-octahydro-1H-inden-4-yl)propanoate--CoA ligase
                     FadD3"
                     /protein_id="HET7074780.1"
                     /translation="SDDRYLCINPFFHNFGYKAGILACLQTGATLIPHLTFDPLRTLQ
                     AIERLRISVLPGPPTIYQTLLDHPARRDYDLSSLRFAVTGAATVPVVLVERMQSELDI
                     DIVLTAYGLTEANGMGTMCRPDDDAVTVATTCGRPFADFELRIASTCSGEPGEVLLRG
                     PNVMLGYLDDPEATAAAIDADGWLHTGDIGAVDKAGNLRITDRLKDMYICGGFNVYPA
                     EVEQVLARMDGVADAAVIGVPDERLGEVGRAFVVLRPGSNLDEQSVIAYTREYLANFK
                     APRAVRFVDALPRNAGGKVVKPQLRELG"
     gene            917..2050
                     /locus_tag="VFI55_11955"
     CDS             917..2050
                     /locus_tag="VFI55_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7074781.1"
                     /translation="MDLTFDEETLAFQAEVRDFLSANADSIPTASYDNAEGFAQHRHW
                     DRVLFDAGLSVITWPKKYGGRDAPLLQWVVFEEEYFRAGAPGRASANGTSMLAPTLFA
                     HGTEEQRDRILPKMASGEQIWAQAWSEPDSGSDLASLRSTATKTDGGWLLNGQKIWSS
                     RAPFAEMAFGLFRSDPSAERHDGLTYLMFDLDAAGVTVRPIVQLGGDTGFGEIFLDDA
                     FVPDEDVIGAPNDGWRAAMSTSSNERGMSLRSPARFLAAAERLVRLWKDSGSPTPFAD
                     RVADAWIKAQAYRLQTFGTVTRLAAGGELGAESSVTKVFWSDLDVAIHQTALDILAAD
                     GELAGPWTDGLLFALGGPIYAGTNEIQRNIIAERLLGLPREKK"
     gene            2047..3009
                     /locus_tag="VFI55_11960"
     CDS             2047..3009
                     /locus_tag="VFI55_11960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7074782.1"
                     /translation="MTVEFGLDEQQRDFAVSIDAALGAADLPGAVRAWSAADVAPGRK
                     VWELLAHLGVTALAVPEKFDGLDAHPVDLVVALERLGRWCVPGPVSESIAVAPVLLAD
                     DERSAGLASGELIATVAMPPQVPYAVDAGTAGLVLLATDEGVSEAVVVGGRTAAYQSV
                     DPSRRLYDVTAAGEVWHPPKGAVKRAYEFGALATAAQLIGAAEALLNEAVDYAKQRSQ
                     FGQVIGSYQAIKHKLADVHIAIELARPLVYGAALSLEARDVSAAKAAASEAALLAARA
                     ALQTHGAIGFTQEHDLSLWLLRVQALRSAWGTPEGHRRRVLEAL"
     gene            3006..3992
                     /locus_tag="VFI55_11965"
     CDS             3006..3992
                     /locus_tag="VFI55_11965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7074783.1"
                     /translation="MTEERRMLRDTVAALVTKHASPVAVRAAMESDRGYDESLWRLLC
                     EQVGAAALVVPEELGGAGGELADAATVLQELGRALVPTPLLGTTLAELALLAAPEPDT
                     ETLATLASLAEGSSIGALVLDADYVVNGDIADVVVAAPHGPGGQLSRWTRFSAQPVAT
                     MDPTRRLARVRPEQTQAIGPDPGLADQAAVLLAAEQIGAAERCLELTVEYAKSRVQFG
                     RPIGSFQALKHRMADLYVAVAAAKSVVSDPCDDPTRTNAATARLAASEALCSVAAEGI
                     QLHGGIAITWEHDMHLYFKRAHGSAQLLGSRRDLLRQLESEVLGAGQVSAQS"
     gene            3989..5155
                     /locus_tag="VFI55_11970"
     CDS             3989..5155
                     /locus_tag="VFI55_11970"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7074784.1"
                     /translation="MTDRVALRAGIPPFYVMDVWLAAAERQRSHGDLVNLSAGQPSVG
                     APEPVRAAAAAALHLNELGYTVALGIPELRAAIAADYRRQHGIAVESDAVIITTGSSG
                     GFLLTFLACFDVGDRVALASPGYPCYRNILSALGCEVVEIPSGPDTRFQPTAQMLAEL
                     DPPVQGVIVASPANPTGTVIPPAELAAIASWCDDRGVRLISDEVYHGLVYEGAPQTSC
                     AWQTSRNAVVVNSFSKYYAMTGWRLGWLLVPAELRRAVDCLTGNFTICPPVLSQIAAV
                     AAFTPEAAAEADGNLHHYTVNRSLLLDGLRRVGIDRLAPTDGALYVYADVSDFTGDSL
                     GFCSKLLSDTGVAIAPGIDFDTARGNSSVRLSFAGPTSDIEEALRRFGPWLSSL"
     gene            complement(5156..7003)
                     /locus_tag="VFI55_11975"
     CDS             complement(5156..7003)
                     /locus_tag="VFI55_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HET7074785.1"
                     /translation="MDELVAPASHGADSHGIGDGTIVVPEGATLTAYFERNRAEHGDS
                     PAYRFIDYSQDQDGRAVELSWNELWSRVCAIAARLQQVTKPGDRVAILAPQGLEYVAA
                     FFGGIQAGNIPVPLFAPTLSGHAERLAAVLADARPAAVLTTTAAAESVRTFIRTLLPN
                     ERPRLIAVDALPATVGSMFSGPAIGTDDVAYLQYTSGSTRTPAGVEISHRNLYTNAMQ
                     MILAGGLDLEVRTVSWLPLYHDMGLMMIMFTALFGAHVTLMDPMAFLRRPYRWIKQLG
                     MEARFGRTFAAAPNFAFELTAERGLPPKGPEAEALDLSNVVCVLNGSEPVTMAAIEKF
                     TNAFAPYGLPVAAIKPSYGMAEATLSVASIGQDAAASAISLDRDQLGSGRAVTVAPTA
                     PGAVTLVSCGQPIADQWAVIASSDGAEMPDGAVGEIWLYGNNIARGYFGREEETRRVF
                     GNKLQSRLDSGSHAAGAPDNCCWLATGDLGVYVDGELYLTGRIKDLIIIDGSNHYPHD
                     IETTVSEASKAIRTGYVAAFSVPADALDSPSGQAGEQVVVVAERAAGAGRTNAEEVFE
                     VVRAAVSRAHRIRVADVRLVAAGTIPRTTSGKLARNACRAEYIAGRFNR"
     gene            complement(7361..8197)
                     /locus_tag="VFI55_11980"
     CDS             complement(7361..8197)
                     /locus_tag="VFI55_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HET7074786.1"
                     /translation="MPLDLTAYFDRVNYSGAAEPNLDVLRDLVTAHTRTIPFENLDPL
                     LGVPVYDLGPGALVDKLVHRRRGGYCYEQNGLMGYVLAELGFRVRRLAGRVVWTLRPD
                     TSPPSQTHTVLAVTFPGSNGSYLVDVGFGGQTPTSPIRLETGTVQQATREPYRLDDRG
                     DGLVLQAQVRGEWQPLYEFTTRSRPQIDLQVGSWFVSTHPTSHFVTNLMVALATDEAR
                     LNLADGKLTIHRATGSEEMGFDNPADVVDTLRDRFGINVADVGERSVLEARIDRLLGV
                     RT"
     gene            8306..8629
                     /locus_tag="VFI55_11985"
     CDS             8306..8629
                     /locus_tag="VFI55_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074787.1"
                     /translation="MRSCRNAFGTVKRRCDEIVEYLDSLFQDQNFNRGELAGPPETSF
                     RVLTAEVVLVSAHLRVKGQGLVGGGTIDRDNFSLRALQRQDDGSWLIVSEMFQDANTE
                     TTYEH"
     gene            8743..9216
                     /locus_tag="VFI55_11990"
     CDS             8743..9216
                     /locus_tag="VFI55_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074788.1"
                     /translation="MIAAVAAVLLIAVLIFAVSRASRRRRERHAEEIREQARLETAKV
                     ERREALAQETAAKARAAQAEAEVKAAEAARLQERAAKHQSDASTSREQLHEQWERAND
                     LDPKVKADSNKAAGRPTSDRDAAWSDERPTDATNAEVPRQGASVNYRDADSWRSN"
     gene            complement(9268..9606)
                     /locus_tag="VFI55_11995"
     CDS             complement(9268..9606)
                     /locus_tag="VFI55_11995"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HET7074789.1"
                     /translation="MRRLAAALVTEDDALLHRARKAAKRARYAAELCRPLGKGAVRKI
                     KYYKKIQSLLGDHQDTVVATEALWRMTLAAGTTLGENGFSYGILYSREQQLARQSRHD
                     VRQFAEAVAR"
     gene            complement(9640..10167)
                     /locus_tag="VFI55_12000"
     CDS             complement(9640..10167)
                     /locus_tag="VFI55_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET7074790.1"
                     /translation="MLGQFCTGITIITTVHDDVPNGFACQSFAALSLEPPLVLFCPTK
                     VSRSWQAIEASGRFCVNVLTEKQQHVSARFGSKEPDKFAGIDWHPSQLGSPIIDGSLA
                     HIDCTVASVHDGGDHFVVFGAVQSLSEAPKIKPRPLLFYRGDYTGIEPDKTTPAQWRD
                     DLEAFLTATTQDTWL"
BASE COUNT         1785 a         3299 c         3367 g         1723 t
ORIGIN      
        1 cagcgacgat cgctacctgt gcatcaatcc gttcttccat aacttcggct acaaggccgg
       61 catcctggcc tgcctgcaga ccggcgcgac gctgatcccc catctgacgt tcgatccgct
      121 gcgcacgctg caggcgatcg aacgactgcg catcagcgtg ttgcccgggc cgccgaccat
      181 ctatcagact ctgctggatc atccggcgcg tcgcgattac gacctgagct cgctgcggtt
      241 cgcggtgacc ggggcggcca ccgttccggt agtgctggtg gaacgcatgc agtccgaact
      301 cgacatcgac atcgtgctga ccgcctacgg actaaccgag gcgaacggga tgggaaccat
      361 gtgccggccc gacgacgacg ccgtcaccgt cgccaccact tgcggacgcc cctttgcgga
      421 tttcgaattg cggatcgctt caacatgttc cggcgaaccc ggagaagtcc tgttgcgcgg
      481 gccgaacgtg atgctgggct acctcgacga cccggaagcg accgccgcgg ccatcgacgc
      541 cgacggctgg ctgcacaccg gtgacatcgg tgccgtcgac aaggccggca atctgcgcat
      601 caccgaccgg ctcaaggaca tgtacatctg tggcggattc aacgtctacc ccgccgaggt
      661 cgagcaggtg ctggcccgga tggacggggt ggccgacgcc gcggtgatcg gcgttcccga
      721 cgagcggctc ggtgaggtgg gtcgggcgtt cgtggtgctg cggcccggct cgaatctcga
      781 cgagcaatcg gtaatcgcct acacccgtga atatttggcg aacttcaagg caccgcgtgc
      841 ggtgcgattc gtcgacgcgt tgccgcgcaa cgcgggcggc aaggtggtca aaccacaact
      901 gcgagagctg ggctgaatgg acctgacatt cgacgaagaa acgttggcct tccaagccga
      961 ggtgcgagac ttcctctccg ccaacgccga ttcgataccg acggcgtctt acgacaacgc
     1021 ggaaggcttt gcacaacaca ggcactggga ccgcgtgttg ttcgacgctg gtctgtcggt
     1081 gatcacctgg ccgaagaaat acggcggccg cgacgctccg ctgttgcagt gggtggtgtt
     1141 cgaagaggag tacttccgcg ccggcgcacc cggccgggcc agcgcgaacg gcacctccat
     1201 gctggcgccg acactgttcg cgcatggcac cgaagaacag cgtgatcgga tactgccgaa
     1261 aatggctagc ggcgagcaga tctgggctca ggcctggtcc gagcccgatt ccggcagcga
     1321 cctggcgtcg ttgcggtcca ccgcgaccaa gaccgacggc ggctggctgc tgaacgggca
     1381 gaagatctgg agctcacgag cgccgttcgc cgagatggcc ttcgggctgt tccgttccga
     1441 cccctcagcg gagcgacacg acggcctgac gtacttgatg ttcgacctgg atgccgcggg
     1501 cgtcaccgtg cgacccatcg tccagctcgg cggggacacc ggcttcggtg agatcttcct
     1561 cgacgacgca ttcgtgcccg acgaggacgt catcggcgcc ccgaacgacg gctggcgtgc
     1621 ggccatgagc acgtcgagca acgagcgtgg catgtcgctg cgcagtccgg cccgctttct
     1681 ggcggccgcc gagcggctgg tgcggctgtg gaaagacagc ggctccccca caccctttgc
     1741 cgatcgggtc gccgacgcgt ggatcaaggc gcaggcctac cgactgcaga ccttcggcac
     1801 cgtgacaaga ctggcggcgg gcggcgaact cggcgccgaa tcgtcggtga ccaaggtgtt
     1861 ctggtccgat ctcgacgtgg cgatccatca gaccgcgctc gacatcctcg ccgccgacgg
     1921 ggagcttgcc gggccgtgga ccgatggcct gttgttcgcg ctgggcggcc cgatctatgc
     1981 cggtaccaac gaaattcagc gcaacatcat cgccgagcgg ttgctgggcc tgccgcgaga
     2041 gaagaagtga ccgtggaatt tggactcgac gaacaacagc gcgacttcgc cgtcagcatc
     2101 gacgccgcgc tcggtgccgc ggacctgccc ggtgcggtgc gtgcgtggtc ggcggccgat
     2161 gtcgcgcccg gccgcaaggt gtgggaattg ctggcccacc tcggcgtcac cgcgctggcg
     2221 gtccccgaga agttcgacgg cctggacgcc cacccggttg acctggtggt cgcgctcgaa
     2281 cgtcttggcc gctggtgtgt gcccgggccg gtcagcgaat cgatcgctgt tgcaccggtt
     2341 ttgcttgctg acgatgaacg cagcgccggg ctggcttccg gcgagctgat cgccactgtc
     2401 gcgatgccgc cacaggttcc gtacgccgtc gacgcgggca ccgcagggtt ggtgctccta
     2461 gccaccgatg agggtgtcag cgaagccgtg gttgttggcg ggcggacggc cgcctaccag
     2521 tccgtcgatc ccagccgccg cctctacgac gtgacggcgg ccggcgaggt ttggcatcct
     2581 ccgaaaggag cagtcaagcg cgcgtacgag ttcggggcgc tggccactgc cgcccagttg
     2641 atcggcgccg ccgaggcact gctgaatgag gccgtcgact acgccaagca acggagccag
     2701 ttcggccagg tgatcgggtc gtaccaggcg atcaaacaca agctcgccga tgtgcacatc
     2761 gccatcgagc tggcccgccc tttggtgtac ggagcggcct tgtcgttgga ggcccgcgac
     2821 gtgagcgcgg cgaaagcggc ggcctccgag gcggctttgc ttgccgcgcg tgcggcgctg
     2881 cagacccacg gtgcgatcgg gttcacccag gagcatgatc tgtcactgtg gctgctgcgc
     2941 gtgcaggcat tgcgatcggc ctggggcact cccgaggggc atcgccgccg agtgttggag
     3001 gcactatgac cgaagaacgg cgcatgttgc gggacaccgt cgccgccctg gtcaccaaac
     3061 acgcaagccc ggtggcggtg cgcgcggcga tggagtccga ccgcggctac gacgaatcgc
     3121 tgtggcggct gctgtgcgag caggtcggcg ctgcggcgct ggtagtcccc gaggagttgg
     3181 gtggtgcggg cggcgaatta gcggatgcgg caacagtttt gcaagagctc gggcgcgcgc
     3241 tggtgcccac tccactgttg gggacgacgc tggccgagct ggcgctgttg gccgcgccgg
     3301 aaccggacac cgaaacgctc gcaacgctgg cgtcgcttgc cgagggcagt tcgatcgggg
     3361 cgctggtact ggacgccgac tacgtggtca acggcgatat cgccgacgtc gttgtcgccg
     3421 ccccccacgg cccgggcggc cagttgagca ggtggacccg attcagtgcg caaccggtcg
     3481 ccaccatgga ccccacccgg cgactggccc gagtgcgccc cgagcagacc caagctatcg
     3541 gccccgaccc gggcctcgcc gaccaagcgg cggtcttgtt ggccgccgag cagatcggtg
     3601 ccgccgaacg ctgcctggaa ctgaccgtcg aatacgccaa gagccgagtc caattcggcc
     3661 gcccgatcgg cagcttccag gcgctcaagc atcggatggc cgatctgtat gtagcggttg
     3721 cggccgccaa atccgtcgtg tccgacccgt gcgatgaccc cactcgcacc aatgccgcca
     3781 ccgcgcgcct ggccgccagc gaggcgctgt gtagtgtggc cgccgaaggc attcagctac
     3841 acggtggtat cgcgatcacc tgggaacacg atatgcactt gtacttcaaa cgcgcgcacg
     3901 gcagcgcgca actgctcggc tcgcgacgag acctgctgcg ccaactggaa tccgaagtgc
     3961 ttggagccgg gcaagtgtcg gcacagtcgt gaccgaccgg gtggcattgc gcgccggcat
     4021 cccgccgttc tatgtgatgg atgtctggct ggccgcagcg gagcgtcagc gcagtcatgg
     4081 cgatctggtg aacctgtcgg cgggccagcc cagcgtgggt gcccccgaac cggtgcgggc
     4141 ggccgccgcg gccgccttgc atctcaacga gctgggttac accgtggcac tgggcattcc
     4201 cgagctgcgg gccgcgatcg cggcggatta ccggcgccag cacggaatcg ccgtcgagtc
     4261 cgacgctgtc atcatcacca ccggttcgtc gggcggtttt ctgctcacct tcctggcatg
     4321 cttcgacgtg ggcgatcggg tggcgttggc cagccccggc tacccgtgct atcggaacat
     4381 cttgtcggcc ttgggatgtg aggtggtgga gatcccgagc ggacctgata cccggttcca
     4441 gcccacagcg cagatgctcg ccgaactcga cccgccggtg cagggcgtga tcgtcgccag
     4501 cccggccaat cccaccggaa cggtcatacc gccggccgag ttggccgcga tcgcttcctg
     4561 gtgtgatgac cgcggggtgc ggctgatcag cgatgaggtc taccacggcc tggtctacga
     4621 aggcgcgcca caaaccagct gcgcctggca aacatcgcga aatgccgtgg tggtgaacag
     4681 tttttccaag tactacgcga tgacgggctg gcggctgggc tggttgctgg tgccggccga
     4741 gctgcgccgc gccgtggact gcctgacggg taacttcacc atctgcccgc cggtgctgtc
     4801 gcagatcgcc gcggtggcag cgttcacccc cgaggcggcc gccgaagcag acggaaatct
     4861 gcatcactac acggtcaacc gctcgctgct gctcgacggc ctgcgccgcg tcgggatcga
     4921 ccggctggcg ccgaccgatg gcgccttgta cgtctacgcc gatgtctcgg acttcaccgg
     4981 cgactcactt ggattctgct caaagttgct gtccgacact ggcgttgcca tcgcgccggg
     5041 catcgacttc gacaccgccc gagggaattc gtccgtccgg ctgtcgttcg ccgggccgac
     5101 gagcgacatc gaagaggcgc tgcgcagatt cgggccctgg ctgtcgagcc tgtagtcagc
     5161 ggttgaatcg ccccgcgatg tactcggccc ggcaggcgtt gcgggccagc ttgccactag
     5221 tcgtgcgggg gatcgtgccc gcggcgacca gccgcacatc ggcaacccgg atgcgatggg
     5281 cgcgcgagac ggctgcccgt acgacctcga agacctcttc cgcgttggtc cggcccgccc
     5341 ccgccgcacg ctcggcgacg acgaccactt gctcgcccgc ctgaccactc ggcgagtcga
     5401 gcgcgtccgc aggaacggag aacgcggcga catagccggt gcggatggct ttcgacgctt
     5461 cgctgaccgt ggtctcgatg tcgtgtgggt agtggttgct gccatcgatg atgatgaggt
     5521 ctttgatccg cccggtcaga tacagttcgc catcgacgta gacaccgaga tcaccggtag
     5581 ccagccagca gcagttgtcc ggagcgccgg cggcatggct gccggagtcg agccgagact
     5641 gaagtttgtt gccgaacacc cgtcgcgttt cttcctcgcg gccgaagtat ccgcgggcga
     5701 tgttgttgcc gtacaaccag atttccccga ccgcaccatc gggcatctcg gcgccgtccg
     5761 atgaggcgat caccgcccac tgatcggcga tcggctggcc gcaggacacc agggtgaccg
     5821 cacccggtgc ggtgggcgcc acggtcaccg cgcgaccgga gccgagctga tcgcggtcca
     5881 aggagatggc gctggccgcc gcgtcctggc cgatactggc gaccgacaat gtcgcttcgg
     5941 ccatcccgta tgacggcttg atggctgcca ccggaaggcc gtagggagcg aacgcgttgg
     6001 tgaatttctc gatggccgcc atcgtcacgg gttcggaccc gttcaatacg cacacaacgt
     6061 tgctgaggtc gagggcttcg gcttccgggc ccttcggggg cagtccccgt tcggccgtca
     6121 actcgaaagc gaagttcggg gcggccgcaa aggtccggcc gaacctggcc tccatgccca
     6181 gctgttttat ccaccggtac ggccggcgca ggaatgccat gggatccatc agcgtcacgt
     6241 gggccccgaa cagcgccgtg aacatgatca tcatcagtcc catgtcgtga tacagcggca
     6301 gccagctgac ggtgcggact tccaggtcca gtccgccggc gaggatcatc tgcatcgcgt
     6361 tcgtgtagag gttgcgatgg ctgatctcca ccccggccgg ggtgcgcgtc gagcccgacg
     6421 tgtactgcag gtacgcgaca tcgtcagtgc cgatggcggg cccgctgaac atcgagccga
     6481 ccgtcgcggg tagcgcgtcc accgcgatca accgcggccg ctcgttcggc agcagcgtgc
     6541 ggataaacgt ccgaaccgac tcggcggctg ctgtcgtggt cagtaccgca gcgggccggg
     6601 cgtcggccag caccgccgcc agccgctcgg cgtgccccga caacgtgggc gcgaacagcg
     6661 gaacggggat gttgcccgcc tggatgccac cgaagaaagc ggccacatac tcgaggccct
     6721 gcggcgccag gatcgccacc cgatcccccg gcttggtcac ctgttgtagg cgggcagcga
     6781 tcgcgcagac ccgcgaccac agctcattcc agctgagctc gacagcccgc ccgtcctgat
     6841 cctgcgagta gtcgatgaag cggtatgcgg gactgtcgcc atgctcggcc cggttgcgtt
     6901 cgaaatacgc cgtcaacgtc gcgccttcgg gcaccacgat ggtgccgtcg ccgatgccgt
     6961 gagaatccgc accgtgcgaa gcgggcgcaa ccaattcgtc caacccgaaa tttcgactca
     7021 cgataggaca ggctaatagc tagatttaca cgtgaccaac tgcagactat gacggaaatc
     7081 acaatcacgc gcgatatcca gcccttgcgc attgcctttt ccctgatgag accccccgat
     7141 cgacgacgag atcggaagcg acggcgaagg cgttgtggca gaccagctca cgtttgacag
     7201 gtgtccacca ccgtcagtgt cgatgcaccg gagcgcgcta gggctggaaa atcccacgcg
     7261 cgcaaactca gctacccgga attcgccgaa gtgtgcccgc cgacaaaagc gagcgataca
     7321 aatttgttca gccgcccttg atattcgaat ctgcgatcag ttaggtccga acgccgagca
     7381 gcctgtcgat gcgtgcctcg aggacgctgc gctcgccgac gtcggccacg ttgatgccga
     7441 accggtcacg aagggtgtcg acgacgtcag ccgggttgtc gaagcccatc tcttcggacc
     7501 ccgttgcgcg gtgaatggtc agcttcccgt cggcgagatt cagccgggcc tcatcggtgg
     7561 ccagcgcaac catcagattg gtcacgaaat gcgaggtggg atgggttgag acaaaccagc
     7621 tccccacctg caggtcgatc tgcgggcggg agcgggtagt gaactcgtag agcggctgcc
     7681 actcgccgcg aacctgcgcc tgcagcacca gcccgtcgcc ccggtcgtcg agtcggtacg
     7741 gctcgcgggt cgcctgctgg acggtgccgg tttccaggcg gatgggcgag gtcggagtct
     7801 gcccgccgaa accgacgtcg acgagatacg agccgttcga gccgggaaac gtcaccgcca
     7861 gcacggtgtg tgtctgtgac ggcggcgagg tgtccggacg cagcgtccac accactcggc
     7921 cggccaatcg ccgcacccgg aagccaagct cggccagcac gtaacccatc agcccgttct
     7981 gctcgtagca gtagccaccc cgccgacggt gaaccaactt gtcgaccagt gcccctgggc
     8041 ccaggtcgta gaccggcact cccagcagcg ggtccaggtt ttcgaacgga atcgttcggg
     8101 tgtgggcggt caccagatcg cgcaagacgt cgaggttcgg ctcggcggcg ccgctatagt
     8161 tgacccggtc gaagtatgcg gtcagatcca gtggcataga gccattctgg tccacgtcgc
     8221 gaccggcaag gcacgctcat cgacggccac tcgacaacag gtaactgcgg agcgagcgag
     8281 tcctagcatc ctgtcatgac caattttgcg gagttgccgc aacgcctttg gcacggtcaa
     8341 gcgcagatgc gacgaaatcg tcgagtacct cgacagcctc ttccaagacc agaacttcaa
     8401 tcgcggcgag ctcgcaggtc cgccggagac gagtttccgg gtgctcaccg ccgaagtggt
     8461 cctggtctcg gcgcatctga gggtgaaagg tcaaggactg gtcgggggcg gcacgattga
     8521 ccgggacaac ttctcgttgc gcgccctgca gcgtcaggac gacggatcgt ggctgatcgt
     8581 ctcggagatg tttcaggacg ccaacaccga gaccacctac gaacactaga aacggtggct
     8641 tgttttatat ctgtcgaatt tggggatccg gtagggacgg gtctgtagac caagagaccc
     8701 attgatgacg gagccctgat gagcaccagc actatttgga ttgtgatcgc cgcagtcgcg
     8761 gcggtcctgt tgattgccgt tctaattttc gcggtgagca gagcgtcgcg ccgccgccgt
     8821 gaacggcacg ccgaagagat ccgcgagcag gcgaggctag agaccgccaa agtcgagagg
     8881 cgcgaggcac ttgcgcaaga aacagccgct aaggcgcgcg ctgcacaggc cgaagccgag
     8941 gtcaaggcgg ccgaagccgc caggctgcag gagcgggcgg ccaaacatca aagcgacgcg
     9001 tcaacttcgc gcgagcaact ccacgaacag tgggagcgtg cgaacgacct cgatccgaaa
     9061 gtcaaggctg actcgaataa agctgctggc cggccgacat cagatcggga cgcggcgtgg
     9121 agcgacgaac gaccgaccga cgcaaccaac gcagaggtgc cccggcaagg cgcgtccgtc
     9181 aactaccgcg acgcggatag ctggcggtca aactgatttg gcgcctgata gacctgatcg
     9241 acacggatag accggtggcc cgccccctca gcgggccacc gcttcagcga attgccgaac
     9301 gtcgtgacgg gactggcgag cgagctgctg ctcgcgtgag tacaagatgc cgtagctgaa
     9361 accgttctct cccaacgtcg ttcccgccgc cagcgtcatc cgccaaagcg cctcggtggc
     9421 caccacggtg tcctggtggt cgccgagcag actctgaatc ttcttgtagt atttgatctt
     9481 tcgcaccgcg cccttgccta agggcctgca cagttcggcg gcgtagcgtg cgcgcttggc
     9541 ggccttacgc gcccgatgca gcaaggcatc gtcctcggtg acaagtgcgg cggccagccg
     9601 ccggatcgtc gataagccag gtaccgctcg gcaaaacggc tacaaccaag tgtcctgggt
     9661 ggtcgcggta aggaacgctt cgaggtcgtc gcgccattgg gccggggtgg tcttgtccgg
     9721 ttcgatgccc gtgtagtcac cgcgatagaa cagcagcggt cgtggcttga tcttgggagc
     9781 ctccgacagt gattggaccg cgccgaatac cacgaagtga tccccgccgt cgtgtaccga
     9841 ggccaccgtg cagtcgatgt gggccaacga accgtcgatg atcggtgaac ccagttgtga
     9901 agggtgccag tcaattccgg cgaacttgtc gggctccttg gagccgaatc gcgcggagac
     9961 gtgctgctgc ttttcggtca gcacgttaac gcagaaccgg ccgctggcct cgatcgcctg
    10021 ccaggacctc gataccttgg tcgggcagaa cagcaccagc ggtggctcca gtgacagagc
    10081 cgcaaaagac tggcacgcga agccgttcgg aacgtcgtcg tgcacggtgg tgatgatggt
    10141 gatgcccgtg cagaactgac ccagcacctg ccgg
//
LOCUS       DASSGQ010000272        11487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1113283,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000272 DASSGQ010000000
VERSION     DASSGQ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11487
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113283"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VFI55_12005"
     CDS             complement(<1..736)
                     /locus_tag="VFI55_12005"
                     /inference="COORDINATES: protein motif:HMM:NF016850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcI family methyltransferase"
                     /protein_id="HET7074791.1"
                     /translation="MKPGDMDDQRHDRGFAGKDYIAALQANRPELFAGERPVADRALH
                     VGIIDRWLQNLDILGVDGPDRFQRITNRPWTSEWSAWIASGAGRNVRHAGVHYSYKGL
                     IHLKPAIDLVLYSSLIWDLRPRTILEFGSLQGGSALWLADQLDILCGRDYGDVHSFEL
                     CYECIDPRAAHPHLQFHRADFRDLTTLDQTFLARLNHPWLVIDDAHENVADLARLMAQ
                     FMVEGDYYVVEDYFLGHPRRSSSVRNA"
     gene            complement(1009..1455)
                     /locus_tag="VFI55_12010"
     CDS             complement(1009..1455)
                     /locus_tag="VFI55_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7074792.1"
                     /translation="MTRPVCVITGSAGSLGSALPRSLLATHEVAAIYRQNLPDVASQL
                     RTPADLSGEPPNPGSPSQAYCIHGDLTSDADTRRIVEVVLARFGRIDIVINSAADIRY
                     HGNLLDIGFDSDAVRRQLVTNCVAPMKLVSVIFHEFWKRRPCRRAR"
     gene            1601..2440
                     /locus_tag="VFI55_12015"
     CDS             1601..2440
                     /locus_tag="VFI55_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7074793.1"
                     /translation="MMTTRTERTFDGVGGVRIVYDVWTPDAPPRAVVVLSHGLGEHAR
                     RYDHVAQRFGDAGLVTYALDHRGHGRSGGKRVLVRDISEYTADFDTLVGIATREQHGL
                     KCIVLGHSMGGGIVFAYGVERPDNYDMMVLSAPAVAAQELVSPLMVVAAKVLGALVPG
                     LPVQELDVDAISRDPAVVAAYNEDPLVHHGKVPAGIGRALLQVGETMPQRAPALTAPL
                     LVVHGSDDRLIPVAGSRRLVECVGSTDVELKVYPGLYHEVFNEPERDQVLDDVISWIS
                     KRL"
     gene            2497..3246
                     /locus_tag="VFI55_12020"
     CDS             2497..3246
                     /locus_tag="VFI55_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HET7074794.1"
                     /translation="MTDDKMLARIAALLRQAEGTDNPHEADAFMSAAQRLATASSIDL
                     AVARSHAAKRSPAQAPIQRTITIGTAGTRGLRTYVQLFVLIAAANDVRCDVASNSTFL
                     YAYGFAEDIDASHALYASLVVQMVRASDNYLASGAHRPTPMITARLNFQLAFGARVGQ
                     RLAEARDQATREATKDRRRPPGTAIALRDKEIELRDHYRSASKARGSWQASRASAGYS
                     SAARRAGDRAGRRARLGNSPEMPGARSALGR"
     gene            3243..3773
                     /locus_tag="VFI55_12025"
     CDS             3243..3773
                     /locus_tag="VFI55_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04338 family metallohydrolase"
                     /protein_id="HET7074795.1"
                     /translation="MTRGGTPGRSGGDSRDSQRAKVYAAEEFVRTLFDRAAEHGSPAV
                     EFFGTRLTLPPEARFGSVESVQRYVDDVLALPTVRQRWPQVSPLRVRARRAATAAHYE
                     SRDGAGVIAVPDRDTADWALRELVVLHEVAHHLCHAGPPHGPEFVATICALTELVMGP
                     ELGHVLRVVYAKEGVR"
     gene            3818..5893
                     /locus_tag="VFI55_12030"
     CDS             3818..5893
                     /locus_tag="VFI55_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HET7074796.1"
                     /translation="MTDDERFSLLVAVMGASEMWQLRDERIPPDVPMSAGYVPGIPRL
                     GVPALLMSDAGLGVTNPGYRPGDTATALPAGLALAASFNPSLARTAGDVIGREARSRG
                     FNVQLAGAMNLARDPRNGRNFEYLSEDPLLTATIAAESVNGIQQQGVISTVKHYSLNC
                     NETNRHWLDAVVDPDAHRESDLLAFEMAIERSQPGAVMAAYNKVNGHYAAANHVLINE
                     VLKGVWGYRGWVMSDWGATPSWECALGGLDQDCGAQLDVLLWQSEAFGERLRAAYADG
                     RLPKERLANMVRRILRSMFAVGIDRHEPAPAPDLAAHHEVALRVARQGIVLLTNRGVL
                     PLEPESTARIAVVGGYAQLGVPAGYGSSAVVPPGGYACVIPIGGPGLAAAARKLYVLP
                     SSPLDELRERLPNAQIEFDPGVNPAEAVLAARRADVAIVFAIRAEGEGFDSADLSLPS
                     GQEALIAAVAAVNPNTVVVLETGNPVTMPWHDIVNAIVQAWYPGQAGGQAIAEILVGQ
                     VNPSGRLPITFPVDLSQTPRPELPNLDAPWGTPTTIDYFEGADVGYRWFAKCGQTPMY
                     GFGHGLSYTRFQYHELTVSGGDTISVSFSVVNVGDRDGADVPQLYLTAAPGERCLRLL
                     GFERVELAPGATRRVVIEADPRLIARYDGNAGSWRIRPGGHTVAVGASSVALQLSAEV
                     ELTGRTFGR"
     gene            complement(5932..6078)
                     /locus_tag="VFI55_12035"
     CDS             complement(5932..6078)
                     /locus_tag="VFI55_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallothionein"
                     /protein_id="HET7074797.1"
                     /translation="MASYETGTLLTCGHEGCGCRVRIEVPCHCSGATEPYRCTCGDDL
                     IPVQ"
     gene            6193..6852
                     /locus_tag="VFI55_12040"
     CDS             6193..6852
                     /locus_tag="VFI55_12040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET7074798.1"
                     /translation="MTEKPTPQDVDAFLDSTVIGDDPALTAAVQASNAAGLPTIAVSA
                     QQGKFLSLLTGAIQARRILEIGTLGGFSAIWLARGAGPQGRVVTLEYEPKHAEVARAN
                     LQRAGVADRVEVIVGAALDTLPTLSGDPFDLVFIDADKENYPAYLRWAVRLARPGSVI
                     VVDNVIRDGEILEPEPDDPRVRATRQTLQLMGEHPALDTAVIQTVGAKHWDGFAFALV
                     R"
     gene            7012..7452
                     /locus_tag="VFI55_12045"
     CDS             7012..7452
                     /locus_tag="VFI55_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HET7074799.1"
                     /translation="MSTVHSSIDQHPDLLALRAGYERAAELPSAHLTFGLILLAGLYA
                     AASPWIVGFSSTAQLASCDLIVGLATAFLGYGFATALDRAHGLTWTVPVLGLWLILSP
                     WILQGVILTDGMIWSNVVAGVVLAFLGMTASYFGTRTRATAKSA"
     gene            complement(7489..9213)
                     /gene="ilvD"
                     /locus_tag="VFI55_12050"
     CDS             complement(7489..9213)
                     /gene="ilvD"
                     /locus_tag="VFI55_12050"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET7074800.1"
                     /translation="MAPTTDSAPAQGDADIKPRSRDVTDGLEKAAARGMLRAVGMGDE
                     DFAKPQIGVASSWNEITPCNLSLDRLAKAVKEGVFSAGGYPLEFGTISVSDGISMGHE
                     GMHFSLVSREVIADSVETVMQAERLDGSVLLAGCDKSLPGMLMAAARLDLAAVFLYAG
                     SILPGLARMSDGSEREVTIIDAFEAVGACSRGLMSREDVDAIERAICPGEGACGGMYT
                     ANTMASAAEALGMSLPGSAAPPATDRRRDGFARRSGQAVIELLRRGITARDILTKEAF
                     DNAIAVVMAFGGSTNAVLHLLAIAHEADVALSLDDFTRVGSKVPHLADVKPFGRHVMS
                     HVDHVGGVPVVMKALLDAGLLHGDCLTVTGKTVAENLAAIAPPDPDGKVLRALNDPIH
                     TTGGITILRGSLAPDGAVVKTAGFDSDVFEGTARVFDGERAALDALEDGTITNGDAVV
                     IRYEGPKGGPGMREMLAITGAIKGAGLGKDVLLLTDGRFSGGTTGLCVGHIAPEAVDG
                     GPIAFLRDGDRIRLDVAERVLDVLADPDEFEARRKDFTPPPPRYKTGVLAKYVKLVSS
                     AAIGAVCG"
     gene            9337..9627
                     /locus_tag="VFI55_12055"
     CDS             9337..9627
                     /locus_tag="VFI55_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET7074801.1"
                     /translation="MTTAHGYSQQKDNYAKRLRRIEGQVRGIARMIDEDKYCIDVLTQ
                     ISAVNSALRSVALNLLDEHLSHCVTRAVAEGGNDADDKLAEASAAIARLVRS"
     gene            9757..10992
                     /locus_tag="VFI55_12060"
     CDS             9757..10992
                     /locus_tag="VFI55_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7074802.1"
                     /translation="MSAETATTAARTWTPRIAAQLAVLGAAAFIYVTAEILPVGALPS
                     IARDLQVSVVLVGTLLTWYALVAALTTVPLVRWTAHWPRRRALVVSLVCLTVSQLISA
                     VAPNFAVLAAGRVLCALTHGLLWSVIAPIATRLVPPSHAGRATMSIYVGTSLALVIGS
                     PLTAAMSLMWGWRLAAVCVTVAAAVVTVAARLLLPEMVLSADQLKCVGPRARHHRNRR
                     LIVVSLITMVGVTGHFVSYTYIVEIIREVVGVRGPNVAWVLAAYGAAGVIAVGLVAQP
                     LDRRPKGAVIFCMAGLTAAFALLTALAFGGHHTVTALLVGTGAIVLWGAMATAVSPML
                     QSAAMRNGADDPDGASGLYVTAFQVGIMAGSLGGGLLYERSVALMLTASAALMGVALA
                     GMTANRQMLDIPPASSPNS"
     gene            11109..>11487
                     /locus_tag="VFI55_12065"
     CDS             11109..>11487
                     /locus_tag="VFI55_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074803.1"
                     /translation="MSGWTRAWTRRGPLAALKAVGLASAVGWVLVLSAGPALADPDPA
                     PGDPGVVAAPVGPPAPPPDPLAAPPPPDPLAPPVLAPPPFAPPPWPAPFAQPVLAGPA
                     AGQDPTPFTGTPPFGPPKFNPANG"
BASE COUNT         1843 a         3828 c         3885 g         1931 t
ORIGIN      
        1 atgcgttccg cacggagctg cttcggcgtg ggtggcccag gaaatagtct tcgaccacat
       61 agtaatcgcc ttcgaccatg aactgggcca tcaatcgcgc caaatctgcg acgttctcat
      121 gtgcgtcatc gataaccagc catgggtgat tcagccgggc caagaaagtc tgatcgagcg
      181 tggtcaaatc gcgaaaatct gcccgatgaa actgcaggtg gggatgtgca gctcgcggat
      241 cgatacattc gtagcagagt tcaaacgagt gaacgtcgcc gtaatcgcga ccgcacagaa
      301 tgtcgagctg gtccgccagc catagtgcgc ttcccccctg caggctgccg aactcgagga
      361 tcgttcgcgg gcggagatcc caaatcaggc tcgagtacag caccaggtca atcgcgggct
      421 tgaggtggat caaacccttg taggagtagt ggacaccggc atgacggaca tttcttccgg
      481 cgcccgaggc gatccacgcc gaccattccg acgtccacgg tctgttcgtg attctctgaa
      541 accggtctgg gccgtcgacc ccgaggatgt ccaggttctg caaccagcga tcgatgatcc
      601 cgacatgcaa tgcgcggtcc gccactggcc tttcaccggc gaagagctca ggcctgttcg
      661 cttggagagc ggcaatgtag tccttgccgg caaagccacg atcatggcgt tggtcgtcca
      721 tatctccggg cttcatcacg ggctgcaatt ccgacggacc tcgacgactt cgcctgtccc
      781 gcgcgaggcg atcacctcct gcaccgtgga taccactttc tcggtcggca ctgactccgg
      841 aaaccggact ggacatatgg cattcgatcg cacggaatac ggggacagtt cgttggcgag
      901 gtggaccgtg aggaagttga gcgcggcctt ggaggcactg cagaaggatt cgccgacgct
      961 cggaaaacat acaggcctga cacgctggct atgttgacca cgcgtcgatt atcttgcgcg
     1021 tcggcacggc cttcgtttcc agaattcatg aaagatgacc gacacgagct tcatcggcgc
     1081 aacgcagttg gtgacaagct ggcgccttac cgcatctgaa tcgaagccga tatccaagag
     1141 gttgccgtga tagcggatgt cggcggcgga gttgatgacg atgtcgatgc ggccaaaacg
     1201 ggccagcacg acctcgacga tcctgcgggt gtccgcatcg ctggtcaaat caccgtggat
     1261 gcaatatgcc tggctgggtg agcccggatt tggcggctcg ccgctcaggt cggcgggtgt
     1321 gcgcaactgc gaggcgacat cgggaaggtt ttgccggtat atcgcggcaa cctcatgcgt
     1381 ggccagcagt gagcgcggaa gcgcggaacc gaggcttccc gcgcttccgg tgatgacaca
     1441 aaccggtctc gtcacgtggc tttctgcggg tcaatgccgg tgattccgcc gctcccgcga
     1501 cgcggcgacg tcgctcaacg agcccacgac ccgggtgccc gaaggttggt agacgcgcgt
     1561 ttccgcgggc tccccggacc tgcgagccac tacgctgccc ataatgacta ctcgcactga
     1621 acggactttc gacggcgtcg gcggcgtacg catcgtctac gacgtgtgga cgccggacgc
     1681 cccgccgcgg gcggtggtgg tgctgtcaca cggtcttggc gagcatgccc gccgctacga
     1741 ccacgtcgcg cagcgcttcg gcgacgcggg cctggtcacc tacgcgctgg atcatcgtgg
     1801 acacggccgc tccggcggca agcgggtact ggtgcgcgac atctccgagt acaccgccga
     1861 tttcgacacg ctggtaggca tcgccacccg cgagcagcac ggcctcaagt gcatcgtgct
     1921 cgggcacagc atgggcggcg ggatcgtctt cgcgtacggc gtcgagcgcc cggacaacta
     1981 cgacatgatg gtgctctcgg cgccggcggt ggcggcccag gagctggtgt cgccgctgat
     2041 ggtggtcgcc gccaaggtgc tcggtgctct cgtgcccggt ctgccggtgc aggagctcga
     2101 cgtcgacgcc atctcccgcg accccgcggt ggtggcggcc tacaacgaag atcccctggt
     2161 ccaccacgga aaagtcccgg caggcatcgg tcgtgcactg ctgcaggtcg gcgagacgat
     2221 gccgcagcgg gcaccggcgc tgaccgcgcc gctgctggtg gtgcacggtt ccgacgaccg
     2281 gctcatcccc gtcgccggca gccgccgcct ggtcgagtgt gtcggatcca ccgacgtcga
     2341 gttgaaggtc taccccggcc tgtaccacga ggtcttcaac gagccagagc gcgaccaggt
     2401 gctcgacgac gtgatctcct ggatctcgaa aaggctatga gcggctgccg gctctgccac
     2461 ccaccgagtg tcggatcgct cccgtagctt ggcgtcatga ccgacgacaa aatgttggcc
     2521 cgcatcgccg cgctgctgcg ccaggccgaa ggcaccgaca acccacacga ggcggacgcc
     2581 ttcatgagcg ccgcgcaacg gttggcgacg gcgtcctcca tcgacctcgc ggtggcgcgg
     2641 tcgcacgcgg cgaagcgctc gccggcgcag gcgccgatac agcggaccat caccatcgga
     2701 accgcgggta cccgcgggct gcggacgtat gtgcagctct tcgtgctgat cgcggcggcc
     2761 aatgacgtgc gttgcgacgt ggcgtcgaat tcgacgttcc tctacgccta tgggttcgcc
     2821 gaggacatcg acgcgagcca cgctttgtac gccagcctgg tcgtccagat ggtccgggcg
     2881 tcggacaact atctcgcatc gggagcgcat cggcccacgc cgatgatcac cgcgcggctc
     2941 aacttccagc tcgccttcgg ggcccgggtg ggtcagcgtc tggccgaggc ccgcgatcag
     3001 gccacgcggg aggccaccaa ggaccgccgc cgtccgccgg gtaccgctat cgccctgcgg
     3061 gacaaggaga tcgagctacg cgaccactat cgcagcgcgt cgaaggcgcg cggcagctgg
     3121 caggccagtc gagcgtccgc cggatattcg tcggccgcgc gacgcgcggg tgaccgggcc
     3181 ggccggcgag cgcggcttgg aaacagcccc gaaatgcccg gcgcgcgtag cgcattgggc
     3241 cggtgacacg gggcggtaca ccgggccggt ccggggggga ttctcgggat tctcagcggg
     3301 ccaaggttta tgcggctgag gagttcgtcc ggacgttgtt cgaccgtgcc gccgagcatg
     3361 gatcgcccgc cgtggagttc ttcggcacgc ggctgacgct gccgcccgag gcgcgattcg
     3421 ggtcggtcga atccgtgcaa cgctatgtcg acgacgtgct cgcgctgccc acggtgcggc
     3481 aacgctggcc gcaggtatcg ccgttgcggg tgcgagcgcg ccgggccgcc accgcggcgc
     3541 actacgaaag ccgcgacggg gcaggcgtta tcgcggttcc cgatcgcgac accgccgatt
     3601 gggccctgcg cgagctggtg gtgctgcacg aagtggcgca tcacctgtgc cacgccgggc
     3661 cgccgcacgg ccccgagttc gtcgcgacga tctgtgcgct gacggagctg gtcatggggc
     3721 cggagctcgg gcacgtgctg cgtgtcgtct acgccaaaga gggtgtgcgg tgaccgatct
     3781 ggacgctgac gtgcgggcgc gcgaggtcga agcccggatg accgacgacg aacgattctc
     3841 gctgcttgtc gcggtcatgg gggccagcga gatgtggcag ctgcgcgatg agcgcatccc
     3901 gccggacgtc ccgatgagcg ccgggtacgt gcccgggatt ccccggctcg gtgtcccggc
     3961 gctgctgatg agcgatgccg gcctcggggt caccaacccc ggctaccgcc ccggtgacac
     4021 ggccaccgcg ctgccggccg ggctggcgct ggccgccagt ttcaatccgt cgctggcccg
     4081 caccgcgggc gacgtgatcg gtcgagaggc gcgcagccgc ggattcaacg tgcagctcgc
     4141 gggcgcgatg aacctcgcgc gcgatccgcg caacggtcgc aacttcgaat acctttccga
     4201 ggacccgctt ttgaccgcga cgatcgccgc ggagtcggtc aacgggatcc agcaacaagg
     4261 cgtcatctcg acggtcaagc actactcgct gaactgcaac gagaccaacc ggcactggct
     4321 ggacgcggtc gtcgaccccg acgcgcaccg cgaatccgac ttgctggcct tcgagatggc
     4381 catcgaacgc tcgcagcccg gcgccgtgat ggcggcctac aacaaggtca acggccacta
     4441 cgccgccgca aaccacgtgt tgatcaacga agtgctgaaa ggcgtctggg gataccgcgg
     4501 ttgggtcatg tccgactggg gcgcgacgcc gagttgggaa tgcgcgctgg gcggcctcga
     4561 ccaggactgc ggcgcgcagc tcgacgtgct gctgtggcag tcggaggcgt tcggtgagcg
     4621 gctgcgagcc gcctatgccg acggccggct gcccaaagag cgcctggcga acatggtccg
     4681 gcggattctg cgttcgatgt tcgcggtcgg aatcgaccga cacgaacccg ctcccgcacc
     4741 ggatctggcg gcacaccacg aggttgcgct gcgggtcgca cggcagggga tcgtgctgct
     4801 gaccaatcgt ggcgtgctgc cgctggagcc ggagtcaacc gcgcgcatcg ccgtcgtcgg
     4861 cggctacgca cagctgggcg tcccggccgg ctacggttcc agtgccgtcg ttccgccggg
     4921 cggctatgcg tgcgtgatcc cgatcggcgg cccgggcctg gcggccgccg cacgcaagct
     4981 gtacgtattg ccgtcgagtc cgctggacga gctgcgagag cgacttccca acgcgcagat
     5041 cgaatttgat cccggcgtca atccggcgga ggcggtgctg gcggcgcggc gagcggacgt
     5101 cgcgatcgtc ttcgcgatcc gcgccgaggg ggaggggttc gactccgccg acctatccct
     5161 gccctcgggc caggaggcgc tgattgccgc cgtcgctgcc gtcaacccga acaccgtcgt
     5221 ggtgttggag accggcaacc cggtgaccat gccctggcac gacatcgtga acgccatcgt
     5281 gcaggcctgg tatccgggtc aggccggcgg tcaggccatc gcggaaatcc ttgtcggcca
     5341 agtgaacccg tcgggccgac tgccgatcac cttccctgtc gacctcagtc agacgccccg
     5401 gccggagctg cccaacctcg acgctccgtg ggggacgccg acgacaatcg actacttcga
     5461 aggagccgac gtcggctacc gctggtttgc caaatgtgga cagaccccga tgtacggatt
     5521 cggtcatggc ttgtcctaca ccaggtttca gtatcacgag ctgacggtga gcggaggtga
     5581 caccatcagc gtcagcttca gcgtcgtcaa cgtcggcgac cgcgacgggg cggacgtccc
     5641 gcaactgtat ctgacggcgg ctcccggcga gcggtgcttg cggttgctgg gcttcgaacg
     5701 ggtggagctc gcgccgggtg ccacccgtcg ggtggtgatc gaggcggatc cgcgactgat
     5761 cgcccgatac gacggcaacg cgggaagctg gcgcatcagg ccaggcggtc acaccgtggc
     5821 ggtgggcgct tcgtcggtcg ccctgcaact gtcagcggag gtcgagctca ccggtcgcac
     5881 gttcggacgg tagtcgggca tgacccgaac gcaccgctgg cgtgtcaccc gctactggac
     5941 ggggatcagg tcgtcgccgc aggtgcagcg gtacggctca gtggcgcccg agcagtggca
     6001 tggaacctcg atgcgaacgc gacagccgca gccctcgtgg ccacacgtca gcaaggttcc
     6061 cgtttcgtag cttgccattt cgaatcaccc ttctgtgtgg tggaccagtt caccatatac
     6121 cccctacccg tatctggtaa atagccccgg ccccgccgga ccgttccggc gtgccgggct
     6181 agcgttggcg gaatgaccga aaaaccaacc ccgcaagacg tcgatgcatt cttggacagc
     6241 accgtgatcg gcgacgatcc ggcattgact gccgcggtgc aggccagcaa cgccgccggg
     6301 ttgcccacca tcgccgtatc ggcgcagcag ggcaaattcc tgtccctgtt gacaggtgcg
     6361 atccaggcgc gccgcatcct cgagatcggc acgctgggtg ggttcagcgc catctggctg
     6421 gcgcgcggcg ccggcccgca gggacgggtg gtgacgctgg aatacgagcc caaacacgcc
     6481 gaggtggcac gggccaatct gcagcgggcc ggcgttgccg accgggtgga agtgatagtc
     6541 ggtgcggcgc tggatacgct gccgacgctc tccggcgacc ccttcgacct ggtgttcatc
     6601 gacgccgaca aagagaacta cccggcctat ctacggtggg cggtgcggct ggctcgtccc
     6661 ggctcggtca ttgtggtgga caacgtaatc cgtgacggcg agatcctgga acccgagccc
     6721 gatgacccca gagtgcgggc aacccgccag acgttgcagc tgatgggcga gcaccccgcg
     6781 ctggacaccg cggtgatcca gacggtcggg gccaaacatt gggacgggtt cgcgttcgca
     6841 ttggtgcggt gagaaaggcc gttaggcgtt tgccggaatg gcacgattcg ggcgagtttt
     6901 tctgcggccg attttcattg ctggcctgcg gcgatcaagc gtaatctttg agcagatgga
     6961 tgggttgcgg tgggcccata ccgcttgctg gataagagaa agggttgcca aatgagtaca
     7021 gtccattcat caatcgatca gcaccccgat ttgttggcgc tgcgcgccgg ttatgagcgc
     7081 gccgccgagt tgccgagtgc gcacctcact ttcggcctga ttctgttggc cggcctgtat
     7141 gcggccgcct cgccgtggat cgtcggattc agctcgaccg cacagcttgc cagctgcgac
     7201 ctcatcgtcg ggctcgccac ggcgttcttg gggtacgggt tcgcgacagc actggaccgc
     7261 gcccacggcc tgacctggac ggtgccggtg ctcggcctgt ggctcatctt gtcgccgtgg
     7321 atcctgcagg gcgtcatcct gaccgacggc atgatctggt cgaatgtcgt cgccggtgtg
     7381 gtgttggcgt tcctcggcat gaccgccagc tacttcggca cccgcacgcg ggcgactgcg
     7441 aaaagcgcct agctgtcgaa gtccgttggg cgggagtcca attcagggct agccgcagac
     7501 cgccccgata gcggccgagc tgaccagctt gacgtacttg gccaatacgc cggtcttgta
     7561 ccgcggcggg ggaggagtga aatcctttcg ccgggcctcg aattcgtccg gatcggccag
     7621 cacgtcgagg acccgctcgg cgacgtccag gcggatccgg tcaccgtcgc gcaggaatgc
     7681 gattggcccg ccgtcgaccg cctcgggcgc gatgtgtccc acacacaggc cggtcgttcc
     7741 gccggagaac cggccgtcgg taagcagcag aacgtctttg ccgagcccgg cgcctttgat
     7801 cgcgcccgtg atcgcgagca tttcgcgcat cccgggccca ccctttgggc cctcgtaacg
     7861 gatgaccacc gcgtcgccgt tggtgatggt gccgtcctcc agcgcgtcca gcgcggcccg
     7921 ctcgccgtcg aaaacccttg cggtgccttc gaacacgtcg gagtcgaaac cggccgtctt
     7981 gaccaccgcg ccgtcgggtg ccagcgaacc gcgcaggatg gtgatcccac cggtcgtgtg
     8041 gatgggatcg ttcagtgcgc gcagcacctt gccgtcgggg tccggcggtg cgatggcggc
     8101 caggttctcg gcaacggtct tgccggtcac cgtcaagcag tcaccatgca gcaagcccgc
     8161 gtccaacagg gccttcatca ccaccggcac gccgccgacg tggtcgacgt gggacatcac
     8221 gtggcggccg aacggcttga cgtcggcaag gtgcggaacc ttcgacccga cccgggtgaa
     8281 gtcatccagt gacagcgcga cgtcggcctc atgggcgatc gccagcaggt gcagcaccgc
     8341 gttggtcgag ccgccgaagg ccatcaccac ggcgatcgcg ttgtcgaacg cctctttggt
     8401 aaggatgtcg cgggcggtga tgccgcgccg cagcaattcg atgactgcct ggccgctgcg
     8461 cctggcgaac ccgtcgcggc ggcggtcggt cgccggcggc gccgcgctgc ccggcaacga
     8521 catcccgagc gcctcggcgg cgctggccat ggtgttggcg gtgtacatgc cgccgcacgc
     8581 accctcgccg ggacagatcg cgcgctcgat ggcgtcgacg tcctcgcgcg acatcagacc
     8641 gcgcgagcac gcgccgaccg cctcgaacgc gtcgatgatc gtgacctcgc gctcgctgcc
     8701 gtccgacatc ctggccaggc ccggcagaat cgagcccgcg tagaggaaca ccgcggccag
     8761 gtccagtcgc gcggcggcca tcagcatccc gggcagcgac ttgtcgcacc cggccaacag
     8821 caccgaaccg tccagccgct ccgcctgcat cacggtttcg acgctgtccg cgatcacctc
     8881 gcgcgacacc agggagaaat gcatcccctc gtgacccatc gagataccgt cggaaaccga
     8941 gatcgtcccg aactcgagcg gatagccgcc cgcggagaac accccttcct tgaccgcctt
     9001 ggcgagcctg tccagggaga ggttgcacgg ggtgatctcg ttccacgacg acgcgacccc
     9061 gatttgcggc ttggcgaagt cctcgtctcc cataccgacc gcgcgcagca tgccgcgggc
     9121 cgcggccttc tccaggccgt cggtgacgtc acgactacgc ggtttgatgt cggcgtctcc
     9181 ttgagcgggc gccgaatcgg tggtgggagc catcgctcaa gtatgcgcga gtcggtcgcg
     9241 ccgcaaccgc gcggggttat accccgatgg ggtatggggt agactcgggt taccgatcgg
     9301 gccggccgcc cgggcaccgg gagggcgttg agagagatga cgactgcaca cgggtattcg
     9361 cagcaaaagg acaactacgc caagcggctg cggcgcatcg agggccaagt gcgtggcatc
     9421 gcacgcatga tcgacgaaga caagtactgc atcgacgttc tcacccagat cagcgctgtc
     9481 aacagtgcat tgcggtcggt ggccctcaat ctgctcgacg agcacctgag ccactgcgtg
     9541 acacgtgcgg tggccgaggg cggcaacgac gcggacgata agctggccga agcctctgcc
     9601 gccatcgcac ggttggtgcg ttcctgatcg ggcacgcgtg ttgaaccgct aagttgggcg
     9661 aacccgttgc cgcagtgcgt acggtaggga cagcgcaatc gggccaggaa cgaccaccca
     9721 gggaaaaggc ccggcggatt gcgcaaaccc acgcccatga gtgccgaaac tgccaccact
     9781 gccgcgcgaa cgtggacgcc acggatcgcc gcgcaactag cggtactggg cgccgccgcc
     9841 ttcatctatg tcaccgctga aattctgccg gtcggcgcgc tgccctcgat cgcgcgagac
     9901 ctgcaagtca gcgtggtgct ggtgggcacc ctactgacct ggtacgcgct ggtggcggcc
     9961 ctcacaaccg tcccgctggt gcgctggacg gcgcactggc cacgccggcg cgcgttggtg
    10021 gtcagcctgg tctgcctgac cgtttcccag ctcatctcgg ccgtggcgcc caatttcgcg
    10081 gtgctcgccg cgggacgggt gctgtgcgcg ctgacccacg gcctgctgtg gtcggtgatc
    10141 gccccgatcg ccacccggct ggtcccgccc agccacgccg gtcgcgccac gatgtcgatc
    10201 tacgtaggca ccagcctggc actggtgatc ggcagcccgc tgacggcggc gatgagcctg
    10261 atgtggggct ggcggctggc cgcggtctgc gtcaccgtcg ctgcagccgt cgtcaccgtc
    10321 gcggcccggc ttctgctgcc cgagatggtg ctcagcgctg accagctcaa gtgtgtgggt
    10381 ccgcgggcgc ggcaccatcg taatcgccgg ctgatcgtcg tcagcctgat cactatggtc
    10441 ggcgtcaccg gccacttcgt gtcctacacc tacatcgtgg agatcatccg cgaagtcgtc
    10501 ggcgtgcgcg gacccaacgt ggcctgggta ctggccgctt atggcgccgc cggcgtgatc
    10561 gcggtggggt tggtggccca acctttggac cgccggccca aaggtgccgt catcttttgc
    10621 atggccgggc ttacggcggc gttcgccctg ctgaccgcgc tcgccttcgg cgggcatcac
    10681 acggtgacgg ccctgctggt cgggaccggc gccatcgtgc tgtggggcgc catggccacc
    10741 gccgtgtcgc cgatgctgca gtccgccgcg atgcgcaatg gagccgacga tcccgacggg
    10801 gcgtcgggcc tgtacgtgac ggctttccag gtgggcatca tggccggctc gctgggtggc
    10861 gggttgctgt atgagcgcag tgtggcgctg atgctgaccg cctcggcggc cctgatgggc
    10921 gtcgcgttgg ccggtatgac ggccaatcgg cagatgctgg acattcctcc agcaagctcg
    10981 cccaattcat aactgcgcag gtcaacgggg cgaattgcgt cgatcaccgg cccggcgccg
    11041 cgcgcagacc gggtagctgg cccgagcgct atgccacact aggtccaaac ctgacgggag
    11101 ggatgcatat gtcgggctgg acgagggcct ggactcggag gggcccctta gccgctctga
    11161 aggcagtcgg attggcttcc gcagtgggct gggtgctggt gctgagtgca gggcccgcgc
    11221 tggcggatcc ggatccggcc cccggcgacc ccggggtggt cgctgccccc gtcggcccac
    11281 ccgcgccacc gcccgacccg ctggcggcgc cgcctccccc agacccgctc gcgccacctg
    11341 tgctcgcgcc accgccgttc gcgccgccgc cgtggcccgc cccgttcgcc caaccggtgc
    11401 tggcgggtcc ggccgccggc caggatccga cgcccttcac cggcacgccg ccgttcgggc
    11461 ccccgaagtt caacccggct aatgggt
//
LOCUS       DASSGQ010000273         5401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1264981,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000273 DASSGQ010000000
VERSION     DASSGQ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264981"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VFI55_12070"
     CDS             <1..218
                     /locus_tag="VFI55_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074804.1"
                     /translation="AEVEEPAHDEAPTGSDVDAEDREDQPEAAPDTTPAEDAGTATDE
                     AKADATTTPGGRARRRFGFLQRNRTRR"
     gene            273..704
                     /locus_tag="VFI55_12075"
     CDS             273..704
                     /locus_tag="VFI55_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HET7074805.1"
                     /translation="MTDQSWNDAAGSGSPSQQPPGYPPPQQPPGYQSGYPPPYPPYPP
                     YQAPGPYYDPSAPFGRHPVTGQPLSDKSKTVAGLLQLLSLIGIGGIGRFYIGDIGIGI
                     AQLLVGWLTCGIGLIWSIVDAVLILTDKVPDSQGRPLRDGT"
     gene            694..1137
                     /locus_tag="VFI55_12080"
     CDS             694..1137
                     /locus_tag="VFI55_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HET7074806.1"
                     /translation="MEPDPASRASRQSRLHGPHGHRPYAAAGTGLLLAGAVGYVGLVD
                     PHNTNSAYPLCPFKWLTGWNCPFCGGLRMTHDLLHGDLMASINDNVFLLVAIPVLAAW
                     LLVRRRRGQSSLPVPAALTITVVAIAWTVLRNLPGFPLVPTVLGG"
     gene            1200..2618
                     /gene="pyk"
                     /locus_tag="VFI55_12085"
     CDS             1200..2618
                     /gene="pyk"
                     /locus_tag="VFI55_12085"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET7074807.1"
                     /translation="MSRRGKIVCTLGPATNSDDLILALVEAGMDVARMNFSHGDHADH
                     KAAYGRVRAASDSTGRAVGVLADLQGPKIRLGRFATGPTFWADGETIRITIDDCEGTH
                     DRVSTTYKRLAKDAVVGDRVLVDDGKVGLLVSSIEGDDVICTVVEGGPVSNNKGMSLP
                     GMNVSAPALSDKDIEDLTFALELGVDLVALSFVRSPSDVELVHEVMDRIGRRVPVIAK
                     LEKPEAVDNLEAIVLAFDGIMVARGDLGVELPLEEVPLVQKRAIQMARENAKPVIVAT
                     QMLDSMIENSRPTRAEASDVANAVLDGADALMLSGETSVGKYPLAAVRTMSRIVCAVE
                     ENSTAAPPLAHVPRTKRGVISYAARDIGERLDAKALVAFTQSGDTVRRLARLHTPLPL
                     LAFTAWPEVRSQLAMTWGTETFIVPMMTSTDGMIRQVDKSLLDLGRYKRGDLVVIVAG
                     APPGTVGSTNLIHVHRIGEDDV"
     gene            2791..3639
                     /locus_tag="VFI55_12090"
     CDS             2791..3639
                     /locus_tag="VFI55_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HET7074808.1"
                     /translation="MLAILDLNRVADDVFIGSHPSKNPMRTFGGQLMAQSFVASSRTL
                     IRDGLPPSALSVHFINGGDIAKDIEFHVVRLRDERRFANRRVDALQDGVLLSSAMISY
                     MSGGSGLEHAVDPPEVPEPHTQPSIGELLRGYEKTVPHFVNALQPIEWRYTNDPSWVM
                     RDKGDRLPHNRVWVKALGALPDDPVLHTALMVYSSDTTVLDSVITTHGLSWGYDRIFA
                     ASANHSVWFHRQVNFDDWVLYSTSSPVAADSRGLGTGHFFDRAGQVIATVVQEGVLKY
                     FPPSRR"
     gene            3790..5187
                     /locus_tag="VFI55_12095"
     CDS             3790..5187
                     /locus_tag="VFI55_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HET7074809.1"
                     /translation="MNGSPLDITAKSRARERVVVHVDSLAARWLGALALLCAASWLVV
                     ILAHHHHRPDWHYVDRLDWSLTVLAAVALIARGIFLGRPVTAMHAAAAAICVVAGIGS
                     HVLSFDLLGDAIIVSSGMVLMWPTSSHARAADLPRVWALINATSDDPLAPFAMQTGKC
                     YHFTADGSAALAYRTRLGFAAVGGDPVGAQDKFPELVADFAAMCHAHGWRIAVVGCSE
                     RRLGLWNDTRVTGRALRAIPIGRDVVVDVSSFSMEGRGFRNLRQAVKRTHNAGITTEI
                     VNEQELDDVRLAELTDVVRASAHGAHTDRGFHMNLDGVLERRFPGIQLIIARDASGRV
                     QGFHRYATAGGGSDITLDIPWRRHGAPNGIDERLSVDMIEAAKDAGAQRVSLSFAAFP
                     EIFEDQNRGWLQRVFYRLIHVLDPLIALESLYRYVCKFHALDARRYALISMTQLVPLA
                     FALLTLEFMPRRRHL"
     gene            complement(5194..>5401)
                     /locus_tag="VFI55_12100"
     CDS             complement(5194..>5401)
                     /locus_tag="VFI55_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7074810.1"
                     /translation="ARAIVSPARIVLLDEPTEHLDAIDAERLLSDLLGPDPRLMSAER
                     TVVVATHHLPSGIRCRELHVDRAG"
BASE COUNT          913 a         1767 c         1780 g          941 t
ORIGIN      
        1 aagccgaggt ggaggagccc gcgcacgacg aagcgcccac cgggtccgac gtcgacgcag
       61 aggaccgaga agaccagcct gaggccgcgc ccgacacgac gcccgccgaa gacgccggaa
      121 cagccaccga cgaagccaag gccgatgcga cgacgacgcc gggcggccgc gcccggcgtc
      181 gattcggctt cctgcagagg aaccgaacgc gtcgttagcg gtcacgtccc gcggccagcg
      241 cagcagcccg gcggatctgg catgctgaaa ccgtgactga ccagtcgtgg aacgacgccg
      301 caggatcagg ctcgccatcg cagcagccac cgggataccc gccaccgcag cagccgccgg
      361 gatatcagtc cgggtatccg ccgccctacc cgccctaccc gccgtatcag gcgcccggcc
      421 cgtactacga cccttccgcg cccttcggcc gccacccggt gaccgggcaa ccgctttcgg
      481 acaaatcgaa gaccgttgcg ggccttttgc agctgctcag cttgatcggc atcggcggga
      541 tcgggcgttt ctacatcggt gacatcggaa tcggcatcgc gcaactgctg gtgggctggc
      601 tcacgtgcgg aatcggtctg atctggagca tcgtcgacgc ggtgctcatt ctgaccgaca
      661 aggtccccga ctcgcagggt cgcccactac gcgatggaac ctgatccggc ttctcgggct
      721 tcgaggcagt ctcgtctcca cggcccgcac ggtcatcgtc cgtacgccgc ggcgggtacc
      781 ggtctgctgc tcgccggcgc ggtcggctat gtcggactgg tcgatccgca caacacgaat
      841 tcggcgtatc cgctgtgccc gttcaaatgg ctgaccggct ggaattgccc gttttgcggt
      901 gggcttcgca tgacgcacga cttgctgcac ggcgatctga tggccagcat caacgacaac
      961 gtcttcctgc tggtcgcgat cccggtgctg gccgcctggc tgctggttcg ccgccgccgc
     1021 ggccagtcgt cgctgccggt gccggcggcg ctgacgatca cggtcgtggc aatcgcgtgg
     1081 acggtgctgc gcaatcttcc tggctttccg ctggttccga ccgttcttgg gggatagtcc
     1141 gccgcggcgg agcaattaac gcgggccctt ccggcttggc gggactatgc tgactcgtcg
     1201 tgagtagacg cgggaagatc gtctgcaccc ttggccctgc aaccaactcc gacgacctga
     1261 ttctggcgct ggtagaggct ggaatggacg tcgcccgaat gaacttcagt cacggcgacc
     1321 atgccgatca caaagccgcc tatggacggg tgcgagccgc ctccgactcc accggtcgcg
     1381 cggtcggtgt gctcgccgat cttcaaggcc caaagatcag gctggggcgc ttcgccaccg
     1441 ggcccacttt ctgggccgac ggtgagacga tacgaatcac catcgacgac tgcgaaggta
     1501 cccacgaccg ggtgtcgacc acgtacaaaa ggttggccaa ggacgctgtc gtcggcgacc
     1561 gggtcctggt cgacgacggc aaggtcggac tgctggtctc cagcatcgag ggcgacgacg
     1621 taatctgcac ggtcgtcgag ggcggcccgg tcagcaacaa caagggcatg tcgctaccgg
     1681 ggatgaacgt gtccgcaccg gccttgtcgg acaaggacat tgaggatctc accttcgcgc
     1741 tggaacttgg tgtcgacctg gttgccctgt cgttcgtgcg ctcgccgtcc gacgttgagc
     1801 tggtccacga ggtgatggat cggatcgggc ggcgggtacc ggtgatcgcc aagctcgaga
     1861 agccggaagc cgtcgacaat ctggaagcta tcgtgctggc cttcgacggc atcatggtgg
     1921 cccgcggcga cctcggcgtc gagctgccgc tggaagaggt tccgctggtg cagaagcggg
     1981 ccatccagat ggcccgggag aatgcgaaac ctgtcatcgt ggcgacccag atgctcgact
     2041 cgatgatcga gaactcgcgg cccacccgag ccgaggcctc cgacgtcgcc aatgccgtgc
     2101 tggacggcgc cgacgcgctg atgctgtccg gggaaacctc ggtgggcaag taccctttgg
     2161 ccgccgtccg gacgatgtcg cgcatcgtgt gcgcggttga ggagaattcc acggctgcgc
     2221 cgccgttggc gcatgtgccc cgcaccaagc gcggagtgat ctcttacgcc gcgcgcgata
     2281 tcggcgagcg actcgacgcc aaggccctgg tcgcgttcac gcaatccggc gataccgtgc
     2341 gacggctggc acgcctgcat accccgctgc cactgctcgc cttcaccgcg tggcccgagg
     2401 tgcgcagcca actcgccatg acctggggca ccgaaacatt catcgttccg atgatgacgt
     2461 ccaccgacgg catgatccgc caggtcgaca agtcgctgct cgacctcggt cgctacaagc
     2521 gcggtgacct ggtggtcatc gtcgccggcg cgccacctgg cacagtaggc tcgaccaacc
     2581 tgatccacgt gcaccggatc ggggaggacg acgtctaacc cggcgacgat gcggcccgcg
     2641 tagcgggctg aggtgggggt acccgccgct tgcgggggag agctggaaaa caaacgagtt
     2701 cgaggagagt ctgtgccggc cggccgtgag tctcaggaac cgaatgcggg agaagaaccc
     2761 gcgacaccat ccacctcgga tttcaaggaa ctgctcgcga tactcgacct caaccgcgtc
     2821 gccgatgacg tgttcatcgg gtcccatccg agcaagaacc cgatgcggac cttcggcggc
     2881 cagctcatgg cgcaatcgtt cgtcgcgagc agtcgcacgc tgatccgcga cggcctgccg
     2941 cccagcgccc tgtcggtgca cttcatcaac ggcggagaca tcgccaaaga catcgagttc
     3001 cacgtggtcc ggttgcgcga cgagcgtcgc ttcgccaatc ggcgcgtgga cgcgctgcaa
     3061 gacggcgtcc tgttgtcctc ggcgatgatc tcgtacatgt ccggcgggag cggcctcgag
     3121 cacgccgtcg atccaccaga ggtcccagag ccccacacgc agccatcgat cggcgagctg
     3181 ttgcgaggct acgagaagac cgtcccgcac ttcgtcaacg ccctgcagcc gatcgaatgg
     3241 cgctatacga atgacccctc ctgggtgatg cgggacaaag gagaccggct gccccacaac
     3301 agggtctggg tcaaggcgct gggcgcgctg cccgacgacc cggtgctgca caccgcgctg
     3361 atggtgtact cctcggacac caccgtgctg gactcggtca tcaccacgca cgggctgtcg
     3421 tggggctacg atcgcatctt cgccgcgtct gccaaccatt ccgtctggtt ccaccggcag
     3481 gtcaacttcg acgactgggt gttgtattcg acgtcgtcgc cggtcgccgc ggattcgcgc
     3541 ggtttgggca ccgggcactt cttcgatcgc gccggacagg tcatcgcgac ggtggtgcag
     3601 gagggcgtgc tgaaatactt tcctccgtcc cgccgatagg gccggtcgac gaaataccag
     3661 caggacaacc gtccgatttc gggtcggtgc gaccgccatc gggcgtacgt tgtaatcagc
     3721 cgggaaattc ggtgcagagg cggcaaatgg gccgcccagc tgcggtgtca gcgcatcggg
     3781 aggtgagacg tgaacgggtc accgctcgac atcaccgcga agtctcgcgc gcgcgaacgc
     3841 gttgtcgtcc acgtggattc gcttgcggct cgctggttgg gggccctggc tctgctctgc
     3901 gcggcgagct ggctcgtcgt gatactcgcc caccaccacc atcgccccga ctggcactac
     3961 gtcgaccggc tggactggtc gctgacggtt ctggcggcgg tcgcattgat cgctcgcggg
     4021 atcttcctgg ggcgtccggt gacggccatg catgcagccg ctgccgccat ctgtgtggtg
     4081 gcggggatcg gctcgcatgt gctgtccttc gacctgctcg gcgacgcgat catcgtgagt
     4141 tcggggatgg tgctgatgtg gccgacatcg tcgcatgcgc gggccgcgga cctgcctcgt
     4201 gtgtgggcat tgatcaacgc caccagcgac gacccattgg cgccattcgc gatgcagacc
     4261 ggcaagtgct accacttcac cgcggacggc agcgccgcgc tggcataccg gacacggctg
     4321 gggttcgccg cggtcggcgg ggatcctgtc ggcgcgcaag acaaattccc ggagttggtc
     4381 gccgatttcg ccgccatgtg tcatgcccac ggctggcgaa tcgcggtggt cggttgcagc
     4441 gagcggcgac tcggactgtg gaatgacacg agggtgaccg gtcgagcgct acgggcaata
     4501 ccgattggcc gcgatgtcgt ggtcgacgta tccagcttct ccatggaggg acgcggattc
     4561 cgcaatctgc gtcaggcggt caagcgcacc cacaatgccg gcatcaccac cgagatcgtg
     4621 aacgagcagg aactcgacga cgtgcgactt gccgagctga ccgacgtcgt gcgggcttcc
     4681 gcccacggcg cgcacacgga tcgtggcttt cacatgaacc tcgacggggt gctggaacgt
     4741 cgattccccg gaatacagct catcatcgcc agggacgcct cgggacgggt gcagggcttc
     4801 catcggtacg caaccgccgg cggcggcagc gatatcacgc tcgatatccc gtggcgccgc
     4861 cacggagccc ccaatgggat cgacgaacga cttagcgtcg acatgatcga ggccgccaaa
     4921 gatgctggcg cgcaacgggt atcgttgtcc ttcgcggcgt tccccgagat ctttgaggac
     4981 cagaatcgcg gttggctgca gcgcgtcttc tatcggctga tccatgttct cgacccgttg
     5041 atcgcgctcg agtcgctgta tcgatatgtg tgcaagtttc acgccttgga cgcccgacgg
     5101 tacgcgctga tatcgatgac gcagctggtt ccgttggcgt tcgcattgct gacgctggag
     5161 ttcatgcccc gccgacgcca cctctgagcg gggttagccc gcccggtcga cgtgtagttc
     5221 gcggcagcga atgccgctgg gcaagtggtg agtagccact acgacggtgc gttccgcgga
     5281 catcagccgt ggatccgggc ccaacaggtc gctgaggagc cgttcggcgt cgatcgcatc
     5341 gaggtgctcg gtgggctcgt ccagcagcac gattcgggcg ggcgaaacga tcgcgcgagc
     5401 c
//
LOCUS       DASSGQ010000274         3762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_49742, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000274 DASSGQ010000000
VERSION     DASSGQ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49742"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VFI55_12105"
     CDS             <1..501
                     /locus_tag="VFI55_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014712171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET7074811.1"
                     /translation="GAAQVVQTALRHGDRAGIVALGGNRPRWLGADIGQRQFYRVLDT
                     VLDAGDRFENTTGTLAPRAAVPAGAIVIAFSTLLDTEFALALIDLRKRGHIVVAVDIL
                     DSSPFEGRQDPLVDRLWALQRSAMYRDMATVGVDIVSWRGDQSLEESMGVLPDQRRRV
                     RGRLRG"
     gene            509..961
                     /locus_tag="VFI55_12110"
     CDS             509..961
                     /locus_tag="VFI55_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014588736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074812.1"
                     /translation="MKPLTRWAGVASSPVFGLLMVGFAAVGSHGPAVVAGAAAALIAV
                     GVGVLFRPVATLAVLLSVATIAVSDTSLVFAAWSGLCAAAYLVSRHTAATSAGAVIWN
                     WPTVIGAVGFTSTGLIAASFPLQLPWLPLAAPLGALGIYVLATRPFLS"
     gene            complement(1017..2606)
                     /gene="nuoN"
                     /locus_tag="VFI55_12115"
     CDS             complement(1017..2606)
                     /gene="nuoN"
                     /locus_tag="VFI55_12115"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HET7074813.1"
                     /translation="MTLPTPSVEYFLLSPMLIVFGVAVAGVLVEAFLPRRIRYGAQVT
                     LTLAGLIAAFVAVVEVSRSVDASGRTAVLGAVAIDRPALFLQGTVLLAAVLAVIFMAE
                     RTQAKTTSKVTVGAAVAGARGLDSFTPQASAVPGSDAEREAERAGAAQTELFPLAMLS
                     VGGMLVFPASNDLLTMFVALEVLSLPLYLMCGLARHRRLLSQEAAMKYFLLGAFSSAF
                     FLYGVALLYGATGTFVLPEIRDALAAHSDSSTALIGVALLSVGLLFKVGAVPFHSWIP
                     DVYQGAPTPITGFMAAATKVAAFGALLRVVYVALPPLHDQWRPVLWTIAILTLAVGTI
                     TAVNQTDVKRMLAYSSVAHVGFILIGVIAGSAAGLSATLFYLVAYSFSTIGAFAIVGL
                     IRNANGVEDATLSHWAGLGQRSPIVGVMFSMFLLAFAGIPLTSGFVGKLAVFRAAAEG
                     GAVPLVVVGVVASGVAAYFYVRVIVSMFFTEATDDTPRVVVPGILSKLAIAVCAIVTV
                     VLGIFPQPVLDLADRAVQLVH"
     gene            complement(2603..>3762)
                     /locus_tag="VFI55_12120"
     CDS             complement(2603..>3762)
                     /locus_tag="VFI55_12120"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET7074814.1"
                     /translation="FYVFWEIMLIPMYFLIGGFGQGAGRSRAAVKFLLYNLFGGLIML
                     AAVVGLYVVTAQHGPGTFDFRDIVAGVASGRYSADPAVIKALFLGFMFAFAVKAPLWP
                     FHRWLPDAAVEATPATAVLMMAVMDKVGTFGMLRYCLQLFPSASTYFRPLIVVLAVIG
                     VVYGAIVAIGQADIMRLIAYTSISHFGFIILGIFVMTSQGQSGSTLYMLNHGLSTAAV
                     FLIAGFLISRHGSRAIADYGGVQKVAPILAGTFLVSAMATLSLPGLAPFISEFLVLLG
                     TFNRYWLAAAVGVTALVLSAIYMLWLYQRVMTGPVARGNERIGDLAPREMIVVAPLIA
                     LLIALGVYPKPMLDLINPAVENTMTTIGQHDPAPTVPPGTRVPRTAEGPHR"
BASE COUNT          704 a         1217 c         1269 g          572 t
ORIGIN      
        1 ggcgcggcgc aggtggtgca gactgcgttg cggcacggcg atcgcgccgg aatcgtcgcc
       61 ctcggcggca accgcccacg ttggctcggc gccgacatcg gccagcgcca gttctaccgg
      121 gtgctggata ccgtgttgga cgccggcgac cgattcgaaa acacaacggg cacactggcg
      181 ccgcgtgccg ccgtccccgc cggggcgatc gtcatcgcct tctccacgct gctcgacacc
      241 gagttcgcgt tggcgttgat cgacctgcgt aaacgcggcc atattgtggt cgccgtcgac
      301 attctggaca gttcgccgtt cgagggccgg caggatcccc tggtggatcg cctgtgggcg
      361 ctgcagcgtt ccgccatgta tcgcgacatg gccaccgtcg gtgtcgacat cgtgtcgtgg
      421 cggggggatc agagcctgga ggagtcgatg ggcgtgctgc cggaccagcg gcgtcgggtg
      481 cggggacggc tcaggggatg acggcggcat gaaaccactc acacgctggg ctggggtcgc
      541 ctcgtcgccc gttttcggcc ttctcatggt cggcttcgcc gccgtcgggt ctcacgggcc
      601 ggcggtcgtc gcgggagcgg cggcagctct gatcgcggta ggcgtcggag tgctgtttcg
      661 gccggtggcg acgctcgcgg ttttgctttc ggtggcaacg atcgcggtgt cggatacgtc
      721 gctggtgttc gccgcttggt ccggactgtg cgccgcggca tacctggtgt cccgccatac
      781 cgccgcgacg tccgcgggcg ccgtcatctg gaactggccg acagtgattg gcgccgttgg
      841 gtttacgtcg acggggttga ttgccgcgtc gttcccgctg cagctgccgt ggttgcctct
      901 ggccgcgccg ctcggagccc tggggatcta tgtcttagcg acgcgcccgt tcttgagctg
      961 acgtgaagtc cgccgaattg gcatcgcggc tgcgtcactg cagcgccact gtggcgtcaa
     1021 tgcaccagct ggacggcccg gtcggccagg tcgagcaccg gctgcgggaa gatccccaaa
     1081 accacggtga cgatggcgca tacggcgatc gcaagcttgc tcaagatgcc cggtaccacc
     1141 acccgcgggg tgtcgtcggt tgcctcggtg aagaacatcg aaacgatgac ccgcacatag
     1201 aagtacgcag cgacaccgct ggcgaccaca ccgacgacca ccagcggaac cgcgccgccc
     1261 tcggcggcgg ccctgaacac cgccagcttg ccgacgaaac cactcgtcag cgggatgccg
     1321 gcgaaggcca acagaaacat cgaaaacatc acgcccacaa tgggtgaacg ctgcccgagc
     1381 cccgcccaat gcgacagcgt ggcgtcttcc actccgttgg cgttgcggat caggccgacg
     1441 atggcgaacg cgccgatggt gctgaagctg taggcgacca gatagaacaa cgtggcggaa
     1501 agacccgccg cactaccggc gattacaccg atgaggatga agccgacgtg tgcgaccgac
     1561 gaataggcga gcatcctctt gacgtcggtc tggttgaccg cggtgatggt ccccaccgcc
     1621 aaggtgagga tcgcaatcgt ccacagcacc ggacgccact gatcgtgtag cgggggcaat
     1681 gcgacgtaga ccacccggag cagcgccccg aacgccgcga ccttggtggc cgccgccatg
     1741 aatcccgtga tcggggtggg tgcaccctgg tagacgtcgg gaatccagga gtggaacggg
     1801 accgcgccca ctttgaacag cagccctacc gacagcagcg ccacgccgat cagcgccgtg
     1861 gagctatcgc tgtgggccgc aagcgcatcc cggatctcgg gcaggacgaa cgtgccggtc
     1921 gcgccgtaga gcaatgccac gccatagagg aagaacgccg acgagaacgc gcccagcagg
     1981 aagtacttca tcgcggcttc ctgggacaac agacggcgat gacgggccag cccgcacatc
     2041 aggtacaggg gcagcgaaag aacttccagc gcaacgaaca tcgtcaacag gtcgttggac
     2101 gcgggaaaca ccagcatgcc gccgacggac agcatcgcca gcgggaagag ctcggtttgg
     2161 gcggctccgg cgcgctccgc ctcgcgttcg gcgtcgctgc cgggaaccgc ggacgcctgc
     2221 ggggtgaagg agtccaatcc gcgtgcgccc gccaccgccg cccccacggt caccttgctt
     2281 gtcgtcttgg cttgcgtgcg ttcggccatg aagatgaccg ccagcaccgc ggccagcagc
     2341 acggtgccct gcaagaacag cgccggacga tcaatggcca ccgcgccgag caccgcggtg
     2401 cgtccggagg cgtctaccga gcggctcacc tcgacgaccg cgacgaacgc cgcgatcagg
     2461 ccggcgaggg tgagtgtcac ctgggccccg tagcgaattc gcctgggcag gaaagcttcg
     2521 acgagcacgc cggccaccgc gacgccgaag acgatgagca tcggagacag caggaagtac
     2581 tcgacgctgg gggtggggag ggtcatcggt gcggtccttc ggctgtccgg ggcacgcggg
     2641 tacccggtgg cacggttggc gcggggtcgt gctggccgat ggtggtcatg gtgttttcga
     2701 ccgcgggatt gatgagatcc agcatcggct tggggtagac cccgagcgcg atcagcagcg
     2761 cgatcaacgg tgcgacgacg atcatttcgc gcggcgccag atcaccgatt cgctcgtttc
     2821 ctcgagcgac cggtccggtc atcacccgct ggtagagcca caacatgtag atggccgaca
     2881 gcaccagcgc agtgaccccg accgccgctg ccagccagta ccggttgaaa gtgcctaaca
     2941 ggaccaggaa ttcgctgatg aacggcgcca ggccgggcag cgacagggtg gccatcgccg
     3001 agacgaggaa cgtgcccgcc aggatcggcg ccaccttctg tacgccgccg tagtcggcga
     3061 tcgcgcggct gccgtgccgc gaaatcagga aaccggcgat caggaacacc gccgcggtgg
     3121 acaggccgtg gttgagcata tagagcgtgg acccgctctg tccctgactg gtcatcacga
     3181 aaatgcccag gatgatgaat ccgaagtgcg agatcgacgt gtaggcgatc aggcgcatga
     3241 tgtcggcctg gccgatcgcc acgatcgcgc cgtagaccac cccgatgacc gccaacacca
     3301 cgatcagcgg acggaaatag gttgaggcgc tgggaaatag ctggaggcag tagcgcagca
     3361 tgccgaaggt gccgaccttg tccatcaccg ccatcatgag caccgcggtc gccggtgtgg
     3421 cctcgaccgc ggcgtcgggc agccagcggt ggaacggcca gagtggagct ttgaccgcaa
     3481 acgcgaacat gaagcccaag aacagtgcct tgatcaccgc ggggtcagcg ctgtaccggc
     3541 cggaggcgac gcctgccacg atgtcacgga agtcgaacgt gcccggacca tgctgcgcgg
     3601 taaccacata cagcccgacc accgcggcca gcatgatcaa accgccgaac aggttgtaca
     3661 acaagaactt caccgccgcg cgcgacctgc cggcgccctg accgaaaccg ccgatcagga
     3721 agtacatggg gatcagcatg atttcccaga agacgtagaa ca
//
LOCUS       DASSGQ010000275         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_627128,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000275 DASSGQ010000000
VERSION     DASSGQ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627128"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="VFI55_12125"
     CDS             <1..1290
                     /locus_tag="VFI55_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7074815.1"
                     /translation="PLSYPPGEALLALHRRRGPVINSGVGRHGYTYLLGTEANKFVFA
                     NADAFSWRETFQNLALVDGPTALIVSDGDDHRHRRSVVAPGLRHRQVQDYVQTMVASI
                     DAVIDGWRRGQRLDIYQECRRAVRRSTAESLFGQRMAVHSDFLGEQLQPLLDLTHRLP
                     QVVLLQQRVNSPGWRRAMAARERINDLVDAQIADARAQPKSDDHMLTMLIGGRDDDGY
                     TLSDNEIRDSVISLITAGYETTSGALAWAIYSLLSLPGAWDTAAEEVHRVLGKRPPRA
                     GDLDALTYLNGVVHETLRLYPPAVISARRVMRDLWFDGHRIRAGRLLIFSAYVTHRLP
                     DLWPSPTEFRPLRWEPGAPGYRKPAPAEFIPFSGGLHRCIGAVMATTEMTVMLARLVA
                     RTTLRLPVQRIRAGNFAALAPRPGLTVDVTDEPVSAV"
     gene            complement(1319..>2185)
                     /locus_tag="VFI55_12130"
     CDS             complement(1319..>2185)
                     /locus_tag="VFI55_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HET7074816.1"
                     /translation="GEANEIFIEHAVDLGCEALMGALDEAGLQPQDIDMIATTTVTGV
                     AVPSLDARIAGRVGLRPDVRRVPLFGLGCVAGAAGVSRLHDYLRGAPDEVAVLIAVEL
                     CSLTYPAVKPTVSGLVGSALFGDGAAAVVAVGERRAEQVRAGGPDILDSRSRLYPESL
                     HIMGWNVSSAGLQLVLSPELTTLIDRYLADDVNGFLALHGLTKDDIGAWVSHPGGPKV
                     IDAIANTLELPPEALELTWRSLGEVANLSSASVLHILRDTIAKPPPSGSPGLMLAMGP
                     GFCSELVLLRWH"
BASE COUNT          372 a          766 c          714 g          333 t
ORIGIN      
        1 ccgctttcct acccgcccgg tgaggcgttg ctggcgctgc atcggcgacg ggggccggtc
       61 atcaattccg gggtcggccg gcacggctac acctatttgc tcggcaccga ggccaacaag
      121 ttcgtgttcg ccaatgccga cgcgttcagc tggcgcgaga cgtttcaaaa cctggctctg
      181 gtcgacgggc ccactgcgct gatcgtgagc gacggggatg accaccggca ccgccgcagc
      241 gtcgtggcgc cggggctgcg tcaccgacag gtccaagact acgtgcagac tatggtcgcc
      301 agcatcgacg ccgtaataga cgggtggcgg cgcgggcagc ggctggatat ctatcaggag
      361 tgccgccgcg cggtccggcg tagcaccgcc gaaagcctgt tcggtcagcg catggccgtc
      421 cactcggact tccttggaga gcaactccag ccgctgctag acctcaccca ccggctcccg
      481 caggtggtgc tgctgcagca gcgggtcaac tcgccgggtt ggcgacgggc gatggcggcc
      541 cgggaacgca tcaatgacct cgtcgacgcc cagatcgcgg acgcccgcgc ccagcccaag
      601 tccgacgacc acatgttgac catgctgatc ggcgggcgcg acgacgacgg ctacacatta
      661 agcgacaacg agattcgcga ttcggtcatt tcgctgatca ccgccggcta cgagaccacg
      721 agcggcgcgc tggcgtgggc gatctacagc ctgctgtccc taccgggcgc gtgggacacc
      781 gccgccgagg aggtacatcg cgtgctcggc aaaaggccgc cgagggcggg cgacctcgat
      841 gcgctcacct acctcaacgg cgtcgtccac gagacgctac ggctataccc gcccgccgtc
      901 atctccgcgc gcagggtgat gcgcgacctt tggttcgacg ggcaccgcat ccgggcggga
      961 cggttgctga tcttcagcgc ctacgtcacc caccggctgc ccgacttatg gccctcacca
     1021 accgaattcc ggccgctgcg ttgggagccg ggggcaccgg gctaccgcaa gcccgcaccc
     1081 gctgaattca ttccgttcag cgggggcttg catcgctgca tcggcgcggt catggccacc
     1141 acggagatga ccgtcatgct cgcccggctg gtcgccagga ccacgctgcg gttgcctgtt
     1201 cagcgcatcc gcgcaggaaa cttcgccgca ctggccccca ggccggggct taccgtcgac
     1261 gtcaccgacg agccagtaag cgcggtgtag ccgggcgccg cgggtcgcca ccatccacct
     1321 agtgccagcg cagtagcacc agttctgaac agaatcccgg acccatcgca agcatcagtc
     1381 cggggcttcc gctgggcggc ggcttggcga tggtgtcacg caagatgtgc agcaccgagg
     1441 ccgacgaaag gttggcgact tcgcccagcg accgccaggt caactcgagc gcctcgggcg
     1501 gcaactcgag ggtgttggcg atcgcgtcga tgactttggg accacccgga tggctcaccc
     1561 atgcgccaat gtcgtccttg gtcagcccgt gcagggccag gaacccgttg acgtcgtcgg
     1621 cgaggtagcg gtcgatgagg gtcgtgagct cgggggacag caccagctga aggccggccg
     1681 agctgacgtt ccagcccatg atgtgcagcg actcgggata gaggcggctg cgcgaatcga
     1741 gaatgtcggg cccgcccgca cgaacctgct cggcacggcg ttcaccgacg gccaccactg
     1801 cggcggctcc atcgccgaag agcgcactcc cgaccagtcc ggacacggtc ggtttgaccg
     1861 ccggataggt cagcgagcac aactcgaccg cgatgagaac cgcgacttcg tccggcgccc
     1921 cgcgcaggta gtcgtgcagc cgggacaccc cggccgcgcc cgccacgcaa cccagcccga
     1981 acagcggcac ccggcgcacg tccggccgca agcccactcg tccggcaatt cgggcatcca
     2041 gcgacggaac cgcgacaccg gtgaccgtgg tggtggcgat catgtcaatg tcttggggct
     2101 gtagccccgc ctcgtcgagc gcccccatca gcgcctcaca cccaaggtct acggcgtgct
     2161 cgatgaagat ctcgttggcc tcgcc
//
LOCUS       DASSGQ010000276         7481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_442511,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000276 DASSGQ010000000
VERSION     DASSGQ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442511"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1171
                     /locus_tag="VFI55_12135"
     CDS             14..1171
                     /locus_tag="VFI55_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase"
                     /protein_id="HET7074817.1"
                     /translation="MDSQDRWITLAFTWNGLRALGVSEDSLATFPDEFREGMAARADI
                     LGDTGSNNPRSWVGGLAGEDLHAIAILFARNDAEHQRCTAAHADLVARCPGVRVLSFL
                     DLNATPPFDYAHDHFGFRDRLSQPVIEGAGEEPTPGSGGPLQPGEFILGYPDEEGVIT
                     KLPQPEELSRNGSYMAYRRLREHVGLFRNYLKERTGTAEDQGLLAAKLMGRWPSGAPL
                     VLAPHADDPKLGADPLRNNDFNYKEMDPFGYAAPLGSHIRRLNPRDTAVNMNRRRMIR
                     RGATYGEALPEGAGDDGADRGIAAFIICASLVRQFEFAQNVWINDRNFHELGNERDPI
                     CGTQDGTMEYKIPTRPIRKVLKGLPAFTTLTGGAYFFLPGLNGLRYLAALT"
     gene            1182..2201
                     /locus_tag="VFI55_12140"
     CDS             1182..2201
                     /locus_tag="VFI55_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074818.1"
                     /translation="MSTTQPARTYNQAHLARKHTPGRRRISIYWTWSYPWEAQRDPAA
                     LDNRFSTMTEVRRVAWPNYETPEWDAAHFLQGIAGTLELFHRSTLHFQELTGEVTGHP
                     VAVFQRIDQAGYKLPIDERVLADTDTLMVFGLDHVLGQEEAQPEEVAAIRTWLRREGT
                     CLLLAPHHDVGYTDDAAQRQIEYLHHGDPLVPRQQRFSHYTRSLMTALDVPVYNKWGL
                     RPAVIQGSNQIAPLTGFADLDALRLLDNVTTFNFHPHLPHYELTSPENDALRVLGRQR
                     IDPTRPHPFTDAGGTELNALIWMPPTADRAGDIVLVDSTNFTTLFGGTDSLVNFWRNL
                     SLMRC"
     gene            complement(2217..2585)
                     /locus_tag="VFI55_12145"
     CDS             complement(2217..2585)
                     /locus_tag="VFI55_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015629116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7074819.1"
                     /translation="MSAPDSITTLVEDHDGVSVVSVSGEIDLVTAPALEQTIGAVLAD
                     GPAALVIDLSAVDFLGSVGLKILAATYEKLGKSTEFGVVARGPATRRPIHLTGLDKTF
                     PLYPTLDDALTAVRDGKLNR"
     gene            2739..3641
                     /locus_tag="VFI55_12150"
     CDS             2739..3641
                     /locus_tag="VFI55_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7074820.1"
                     /translation="MSDSPSDFGSTTTVAQTASISTEGLLPQLVQHLRQNRTILREEW
                     ARRITAAELLTAMSPEEIFSEATTVYDSYVEVLETGSVEALQDYARDLSERIIPRGVE
                     TNEVVGIVLLLRDVLARSLFEKYQTDFDLLNRVLDAYEPAANRIANTVAVGFVQERER
                     IIRQQQEAIRELSTPVLQVREQLLILPIIGVLDSQRARQVTEQLLRAIRANRAKVVVI
                     DITGVPTIDSTVANHLVQTVDASGLMGASVIITGLSSEIALTLVTIGLDLSKMNAVGD
                     LQGGIEEAERLLGYEVTRTGETTG"
     gene            3660..4049
                     /locus_tag="VFI55_12155"
     CDS             3660..4049
                     /locus_tag="VFI55_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7074821.1"
                     /translation="MPVPILKQGSILIASVQAALTDSDTERLRQDLMERVSRFRAQGI
                     IVDVTAIDVMDSFAARSLRTIAHMTRLRGADTVIVGLQPEVAFAMVQLGLAFDDMNTA
                     LDLEEGLALLNRQRGAGKSTIGRDGGG"
     gene            4039..4449
                     /locus_tag="VFI55_12160"
     CDS             4039..4449
                     /locus_tag="VFI55_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7074822.1"
                     /translation="MAGDIVVDIYKSDDIVAARKAGHQLALDLGFSLTDVTMIATAIS
                     EIARNITSYAGRGAIRVTVADREGRKALVVRAEDDGPGIADIERAMEDGYSTGRGLGL
                     GLPGARRLMDRLVVDSKIGRGTVVEMWKWVPARA"
     gene            4442..5068
                     /locus_tag="VFI55_12165"
     CDS             4442..5068
                     /locus_tag="VFI55_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET7074823.1"
                     /translation="MHENGRLGPIEWATAGRPLPGERVSGDLAIAIGIGDDAALFGVI
                     DGLGHGPAAETAALRAVDTLTRARAERVEALVQLCHRVLGGTRGVAMTLARVDFGAGA
                     LAWTGVGNVAANVVTKAATGIQIRSSARLTAGIVGYRIPEIRPAQVVSIRAGDLILMT
                     TDGIAEDHLEHIDFAASAAVIAGEILSKHAKETDDAMVLAARHRGTST"
     gene            5065..7296
                     /locus_tag="VFI55_12170"
     CDS             5065..7296
                     /locus_tag="VFI55_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET7074824.1"
                     /translation="MNDGTFAEDFHARYAAALHAYLDARDEDSLAVGYELGRRALQEE
                     ISMLDIIEQHVRLILEHSKDVRLDAPIALEFLLQMLAPLDVATRGFLDGNRRYAEQRA
                     RAEGLADRDKFRTALVNSLQEGFFVADHEGSVIEVNHAFIEILGYPAEGLPYRWPQPW
                     LVDKNTARNQQSLVGRNGSAEYETPIRHRDGHLAWVKVSINAVKATGTDQDVYVGTIR
                     DITAERAFAARESAVLRLATAVAVAKSVAELLAITLEECRTAIDVQRVVAVSWPSGDG
                     EPNVQVAGEPSVSGWRGLDPLLRNTFQDARHQLPLTAKAVDRPDNPGKARGMVAVLSG
                     TGDLALWLELRAPRWISAEDRLLVTVLIGHLSLAMQHVRQFESARETSLTLQRAMLPT
                     VEPPPGFAVCYEPAVPPLEIGGDWYDVLPIGDNRIGIVVGDCVGRGLPAASIMGQLRS
                     SARALLINGAQPALVLEQLDSAASLIPNAYCTTVFLAILDTESGVLQYSNAGHMPAVV
                     AGPEPGQTTVLTDAASVPLAVRRDEPRPQASQVLPVGSTLMLFTDGLVERRHESIDDG
                     IDRATAALAETMKLPLDAVPGAVLRELAPGSGYDDDVAMVIYRHRPTLLRIESDATAE
                     QLVDIRHRLAAWLRAAEIPDELAADIVLVVNEACTNCVEHAYRGHRVGAMLLEADISN
                     GEITTRVSDSGSWKTPPPEPRNGGRGLVLMRALSDRMEIDSNPSGTTVDITFHAPAAA
                     ARH"
     gene            complement(7318..>7481)
                     /locus_tag="VFI55_12175"
     CDS             complement(7318..>7481)
                     /locus_tag="VFI55_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite reductase"
                     /protein_id="HET7074825.1"
                     /translation="LALAAASALTRFGVFEAGKASARDPRYTIEPQKRRLAARRAAGV
                     TGDSITTAG"
BASE COUNT         1361 a         2479 c         2441 g         1200 t
ORIGIN      
        1 cgcacggaag acgatggaca gccaggatcg ctggatcacg ctcgccttca cctggaacgg
       61 cctgcgggcg cttggtgttt cggaggattc actggccacg tttccggacg agtttcggga
      121 gggcatggcc gcgcgcgccg acatcctggg cgataccgga tcgaacaacc cccgcagctg
      181 ggtgggcgga ctggccggcg aggacctgca cgccatcgca atcctgttcg cgcgcaacga
      241 tgccgagcac cagcgctgca ccgcggcgca cgccgacctg gtcgcccgat gccccggcgt
      301 gcgggtgctg tcctttctcg acctgaacgc gacgccgccg ttcgactacg cgcatgacca
      361 cttcggtttt cgggatcgat tgtcgcagcc ggtgatcgag ggcgccggcg aagagccgac
      421 accgggttcc ggcgggccgc tacaacccgg agagttcatc ctcggctacc ccgacgagga
      481 aggagtgatc acgaaactgc ctcagcccga ggaattgtcg cgcaacggca gctacatggc
      541 ctaccggcgg ctgcgggagc acgtcggctt gttcaggaac tacctgaagg agcggacagg
      601 gacggcggag gatcagggcc tgctcgccgc gaagctcatg ggccggtggc cgagcggcgc
      661 accgttggtt ctggcgccac acgctgacga cccgaaactc ggcgcggatc ctttgcgtaa
      721 caacgacttc aactataagg agatggaccc gttcggctat gccgcgccgt tgggttccca
      781 catccgccgg ctgaaccccc gtgacacggc ggtcaacatg aaccgtcgcc ggatgatccg
      841 gcgcggcgcg acctacggcg aggcgcttcc agagggagcc ggcgacgacg gcgcggatcg
      901 gggcatcgcc gcgttcatca tctgcgccag cctcgtccgt caattcgagt tcgcgcagaa
      961 cgtgtggatc aacgaccgca acttccacga attgggcaac gaacgcgatc cgatctgcgg
     1021 aacacaggac ggcacaatgg aatacaagat cccgacccgt cctattcgca aggttctcaa
     1081 aggactgccc gcctttacca cccttaccgg cggggcatac ttctttcttc cgggcctcaa
     1141 cggcctcaga tacctggccg cgctcacctg atggggacgc catgagcacc acgcaacccg
     1201 cgcgcaccta caaccaggcg caccttgccc gcaagcacac gccgggccgt cggcgtatca
     1261 gcatctactg gacatggagc tatccctggg aagcccagcg tgatcccgcc gcgttggaca
     1321 atcggttctc cacgatgacc gaggtgcgcc gggtggcttg gccgaattac gagaccccgg
     1381 aatgggatgc ggcgcacttc ctgcaaggca tcgcgggaac gctggagttg ttccaccgct
     1441 cgacattgca cttccaagaa ctgaccggcg aggtgacggg tcatccggtc gcggtgttcc
     1501 agcgcatcga ccaagccggc tacaaactgc cgatcgacga gcgggtgctg gccgacaccg
     1561 acaccctcat ggtcttcggg ctcgatcacg ttctcggcca agaagaagca cagcccgagg
     1621 aggtcgccgc gattcggacg tggctgcggc gggaggggac ctgcctgctg ctggcgccgc
     1681 atcatgatgt cgggtacacg gacgacgcgg cgcaacgcca aatcgaatac ctacaccacg
     1741 gcgacccgct ggtacctcgc caacagcgct tcagccacta cacgcgctcg ctgatgacgg
     1801 ccctggacgt acccgtctac aacaaatggg ggctccggcc ggcagttatc cagggcagca
     1861 accagattgc tccgttgacc ggtttcgccg acctggacgc gctgcgactc ctcgacaacg
     1921 tcaccacctt caacttccac ccgcacttgc cgcactacga actgacgtcg ccggaaaacg
     1981 acgcgctgcg cgtgctgggg cgtcaacgaa tcgatcccac gcggccgcat ccattcaccg
     2041 acgccggcgg caccgagctc aacgcgctca tctggatgcc gcccaccgcg gaccgggccg
     2101 gcgacatcgt attggtcgac tccacaaact tcaccacgct cttcggtggc accgacagcc
     2161 tggtgaactt ctggcggaat ctctcgctga tgcggtgctg atgcgatagc gcgtgctcag
     2221 cggttgagtt tgccgtcccg cacggccgtc aaggcatcgt cgagcgtcgg gtacagcggg
     2281 aaggtcttgt ccaggccggt cagatgaatg ggcctgcggg tcgccggacc gcgcgcaacc
     2341 accccgaact cggtggactt gccgagcttc tcataggtcg ccgcgaggat cttcagccct
     2401 accgagccga gaaagtccac cgcggaaagg tcgatgacca gcgccgcggg gccatctgcg
     2461 agcaccgcgc cgatggtttg ttcaagggcc ggagcggtga ccagatcgat ttcgccgctg
     2521 acactgacca cggacacgcc atcgtggtcc tcaaccaacg tggtaatcga atcaggagct
     2581 gacaatgccg atcctttgtc cggggcctca agcgtggtgc aagcctaacc tgacgtgcga
     2641 actgcgagaa gaataagccc tcgacacctg cgccgcgcgc acccaggcta gtctccccat
     2701 aggcgcagcg cactgcacga cggggcacgg gaggccagat gtcagattcg ccgtccgatt
     2761 tcggcagcac caccaccgtc gcgcaaaccg ccagtatttc caccgaaggc ctgttgccgc
     2821 agctggtcca acatttgcgc caaaatcgaa cgattctgcg cgaggagtgg gcacgcagaa
     2881 tcaccgcagc cgaacttctg accgcgatgt cgccggagga gatcttctcc gaggcgacga
     2941 cggtgtacga cagctacgtc gaggtactcg agaccggtag cgtcgaggcg ctgcaggact
     3001 acgcacgcga cttgtccgag cgcatcattc cgcgtggcgt cgagacgaac gaggtcgtcg
     3061 gcattgtgtt gctgctgcgt gacgtgttgg cccgttcgct gttcgagaaa tatcagacgg
     3121 acttcgacct gctcaaccga gtcctcgacg cctacgagcc cgcggccaac cgcatcgcca
     3181 acacagtcgc cgtcggcttc gtgcaagagc gcgagcgcat catccgccaa cagcaggaag
     3241 ccatccgaga gctgtcgacg ccggtgctgc aggtgcgcga gcagctgctg attctgccga
     3301 tcatcggtgt gctcgatagt cagcgcgccc gccaggtcac cgaacaactg ctgcgggcca
     3361 tccgcgcgaa ccgcgcgaag gtcgtcgtca tcgacatcac cggtgtgccc accatcgact
     3421 ccaccgtggc aaaccacctg gtgcagaccg tcgatgcctc agggttgatg ggcgcgagcg
     3481 tgatcattac cggcctgtcc tcggagatcg cgctgacgct ggtgacgatc gggctggacc
     3541 tgtcgaagat gaacgccgtc ggtgacctgc agggtggtat cgaggaggcc gagcgcctct
     3601 tgggctacga agtcacgcgc accggcgaga ccaccgggta gccacggcgc gagcaatcca
     3661 tgccagtccc catcctgaaa cagggctcga tcctgatcgc ctcggtgcag gccgcgctca
     3721 ccgactccga caccgaacgg ctacggcagg acctcatgga gcgggtcagc aggttccgcg
     3781 cacagggcat catcgtcgac gtcaccgcca tcgacgtgat ggattcgttc gccgcgcgct
     3841 cgctgcggac catcgcccac atgacccggc tgcgcggggc cgacaccgtg atcgtgggtc
     3901 tgcagccaga ggtggccttc gcgatggtcc aactgggtct ggcgttcgac gacatgaata
     3961 cggcgctgga cctcgaagag gggctggccc tgttgaatcg tcaacgggga gcgggcaaat
     4021 cgacgatcgg gcgcgacggt ggcgggtgac atcgtcgtcg atatctataa gtccgatgac
     4081 atcgtcgcgg cccgcaaagc cggacatcag cttgcactgg atctcgggtt ctcgctgacc
     4141 gacgtcacca tgatcgccac ggcgatctcc gaaatcgcac gaaacatcac cagctatgcg
     4201 ggtcgcggcg ccatccgggt cacggtggcc gaccgggagg gccgcaaggc cctggtggta
     4261 cgcgccgagg acgacgggcc cggcatcgcc gacatcgaac gcgccatgga ggacgggtac
     4321 tcgaccggtc gcgggttagg gctgggcctg cccggggctc gccggctgat ggaccgactg
     4381 gtcgtcgact cgaaaatcgg ccgcggcacg gtcgtcgaaa tgtggaaatg ggttcccgcc
     4441 cgtgcatgaa aacggcaggc tgggaccgat cgagtgggca accgcgggtc gcccgctccc
     4501 gggcgagcgc gtctcgggag acctcgcgat cgcgatcggg atcggcgacg acgccgcgct
     4561 gttcggcgtg atcgacggac tcgggcacgg cccggccgct gaaacggccg cgctgcgcgc
     4621 cgtcgatacg ctcaccaggg cgcgcgccga gcgcgtcgag gccctcgttc agctctgcca
     4681 ccgggtgctg ggcggcactc ggggagttgc catgaccctg gccagggtgg atttcggcgc
     4741 cggcgccctg gcctggaccg gggtgggaaa cgtcgccgcc aacgtagtca ccaaggccgc
     4801 gaccggtatc cagatccgat ccagcgcgcg cctcaccgcg gggatcgtcg gctaccgaat
     4861 accggaaatc aggccggcgc aggtggtttc gattcgcgcc ggcgacctga tcttgatgac
     4921 caccgacggt attgccgagg accacctgga gcacatcgac ttcgcggcat ccgcggcggt
     4981 gatcgccgga gagatcctga gtaagcacgc caaggaaacc gacgacgcga tggtgctggc
     5041 ggcccgccac cggggaacct cgacatgaac gacggcacct ttgcggagga cttccacgct
     5101 cggtatgccg ccgcgctgca cgcctatttg gacgcacgcg atgaggacag cctggccgtc
     5161 gggtacgaac tcggtcgccg tgccttgcaa gaagaaatca gcatgctcga catcatcgag
     5221 cagcacgtgc ggctgattct cgaacactcg aaggatgtcc gactcgacgc gccgatcgcg
     5281 ttggagttcc tgctgcaaat gctggcccca ctcgacgtcg caacgcgggg atttctcgac
     5341 ggcaacaggc gctacgccga gcagcgcgcc cgggccgaag gcctggccga ccgcgacaaa
     5401 ttccgtaccg ccctggtgaa ttcgctgcag gagggattct tcgtcgccga tcacgaaggc
     5461 tcggtgatcg aagtcaacca cgccttcatc gaaatcctcg gctatccggc cgaaggtctg
     5521 ccgtaccggt ggccgcaacc ctggctggtc gacaagaaca cggcgcgtaa ccagcaatcc
     5581 ctggtggggc gcaacggcag cgccgaatac gagacaccga ttcggcatcg ggacggccac
     5641 ctcgcttggg tgaaggtgag catcaacgcg gtcaaggcga ccggcaccga ccaggatgtc
     5701 tacgtcggca cgattcgcga catcaccgcg gaacgcgcgt ttgccgcccg ggaaagcgcg
     5761 gtgctgcggc tcgccaccgc ggtggccgtc gccaagagcg tggcggagtt gctggcgatc
     5821 actctcgagg agtgccgaac ggcgatcgac gtccaacgcg ttgtcgccgt ctcgtggccc
     5881 agcggcgacg gcgaaccgaa cgtccaggtc gccggtgaac cttcggtgtc cggttggcgt
     5941 ggacttgacc cgttgttgcg caacacgttt caggatgcgc gtcaccaact tccgctgacg
     6001 gccaaggccg ttgaccgacc cgacaatccc ggcaaggcgc ggggcatggt cgcggtgctc
     6061 tccggcaccg gagaccttgc cttgtggctg gagctccgcg ccccgcgctg gatcagcgcg
     6121 gaggatcggc ttctggtcac cgtgctgatc ggccacctca gtctggccat gcagcatgtg
     6181 cggcagttcg agagtgcccg cgagacctcg ctgaccttgc agcgcgcgat gctgccgacc
     6241 gtggagccac cgccgggctt tgccgtgtgc tacgaacccg ccgtcccgcc gttggaaatc
     6301 gggggcgact ggtacgacgt gctgccgatc ggcgacaacc gcatcggcat cgtcgtcggc
     6361 gactgtgtgg ggcgcggcct gccggcggcg tcgatcatgg gccagctacg cagctcggcg
     6421 cgcgcactgt tgatcaacgg cgcccagccg gcgctcgtgc tcgagcaact cgactcggcc
     6481 gcgtcgctca ttccgaatgc gtactgcacg acggtctttc tggccatcct ggacaccgaa
     6541 tcgggggtgc tgcagtacag caacgccggc catatgccgg ccgtggtcgc cgggcccgag
     6601 ccgggacaga cgaccgtgtt gaccgacgcc gcgtccgtgc cgctggcggt ccgccgagac
     6661 gaaccgcgcc cgcaagcctc ccaggtgtta ccggtgggct cgacgctgat gctgttcact
     6721 gacggcctgg tggagcggcg acacgagtcg attgacgatg gaatcgaccg cgccacagct
     6781 gctttggcgg agacgatgaa actgccgttg gacgccgtcc cgggcgcggt actgcgggag
     6841 ctggctccgg ggtcagggta tgacgacgac gtcgccatgg tgatatatcg gcaccggccc
     6901 accttgcttc gaatcgaaag cgacgccacc gcagaacaat tggtcgatat ccggcatcgt
     6961 ctggccgcct ggctgcgggc cgccgaaatt ccggacgagt tggccgccga catcgtgcta
     7021 gtggtcaacg aggcctgcac caactgcgtc gagcacgcgt accgcggaca ccgggtaggg
     7081 gcgatgctgc tcgaagccga tatttcgaac ggcgaaatca cgacgcgggt ctcggactcc
     7141 ggttcttgga agacgccgcc gcccgagcca cgcaacggtg gacgcggcct ggtgctgatg
     7201 agggcgctca gtgacaggat ggaaatcgac agcaatccga gcggcaccac cgttgacatc
     7261 actttccacg caccggcagc ggcggcgagg cactgacgca ccgtgttccc gcgcaggtca
     7321 gcccgcggtg gtgatcgagt cgccggttac gccggcggcc cggcgggccg caagcctgcg
     7381 tttttgtggc tcgatcgtgt agcgggggtc gcgggccgaa gccttcccgg cctcgaacac
     7441 gccgaaccgg gtgagcgccg atgccgccgc gagcgccagg c
//
LOCUS       DASSGQ010000277         5370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_900798,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000277 DASSGQ010000000
VERSION     DASSGQ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900798"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFI55_12180"
     CDS             complement(<1..296)
                     /locus_tag="VFI55_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014588017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA containing CoxE family protein"
                     /protein_id="HET7074826.1"
                     /translation="MNTPWMLRGVDLAAFAAALVVRLRGVGVLVSANGQATFVQALRL
                     LVPGTTPALYWAARLTLVNRMEDLSAFDAVFTSVFGPVDTTSPDGATGPAPVLP"
     gene            complement(303..947)
                     /locus_tag="VFI55_12185"
     CDS             complement(303..947)
                     /locus_tag="VFI55_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7074827.1"
                     /translation="MKIANQFTVSAPIEQAWDVLCDMEQVIPLMPGAQLTGHEGDDYL
                     GRVKVKVGPVTSEFSGKVRFVEQDRDQHRAVIDAKGKESRGTGNAAATITALLNADGE
                     HTNVTVDTDLKIVGKLAQFGSGMLQQVSEKLLGQFVESLEAELVAKNTETPPPPESPA
                     ETERVPAPRRAPAAEPEPIDLLQLAGGDRLKKYGAAAAAALAALVLIWVLRRRR"
     gene            complement(961..1848)
                     /locus_tag="VFI55_12190"
     CDS             complement(961..1848)
                     /locus_tag="VFI55_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET7074828.1"
                     /translation="MTFGDVDDVITRFDAHDYLLDTGTASAIYLAVTLGRPLLLEGEP
                     GVGKTTAAKTLAAVLNTPLVRLQCYEGLTANEALYDWNYQRQLLSIRLAEARGAGVSD
                     ISEADLYTERYLVDRPILRCVRHRGPRAPVLLIDEIDRADDEFEALLLEFLGESAVTV
                     PELGTFVAEHPPIAVLTSNRSRDLHDALRRRCLYHWIDYPDPVRAAAIVRRTVPGATA
                     PLIEHATQFVGATRDLDLDKPPGVAEAIDWVAALVSLGVADLVDPAALASLGALAKTP
                     DDRTLIRDAYQTFTECSRT"
     gene            complement(1859..2449)
                     /locus_tag="VFI55_12195"
     CDS             complement(1859..2449)
                     /locus_tag="VFI55_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003900132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET7074829.1"
                     /translation="MTAPPVTGVVLAAGSSRRLGTPKQVLPYRDTTLLGATLEVARSV
                     GFDQLIVTLGGAAPAVRDAVRLDGADVIAVDEPATGCSASLRAALDRVDRRAAGIVLM
                     LGDQPCVEPATVRRMIQHGRAAPAGIMVCRYDDGIGHPFWLGRSVFGDLRQLHGDKGV
                     WKLVESGRHPVRELEVAGPVPLDVDTWDDYRRLVES"
     gene            complement(2446..3354)
                     /locus_tag="VFI55_12200"
     CDS             complement(2446..3354)
                     /locus_tag="VFI55_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005243651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET7074830.1"
                     /translation="MPTISERAQQLLAARTPFVHATVVRAQPPTSAYPGDEAILLADG
                     TIEGFVGGQCAQNSVRKAALGALQAGESVLLRVLPDGDVHFPEAPGACVVVNPCLSGG
                     SLEIFLAPQLPAPLIEIHGGTPIGDALAQLCEVLGYDVRRDTELADAAAPPTAPATAI
                     VIASHGGPEAEIIRAALDNGVGYIGLVASKVRGASILNELELCEAERVRVHTPVGLPI
                     GAKTPAEIAVSIAAELVAGIRGAGGPSLSRAFVLPDSPPEAVDPVCGMTVTVGPATDH
                     LKLAGADYWFCGPGCRTAFANRPVGA"
     gene            complement(3354..>5370)
                     /locus_tag="VFI55_12205"
     CDS             complement(3354..>5370)
                     /locus_tag="VFI55_12205"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HET7074831.1"
                     /translation="YSARDALELIDVDYDPLDPVIDVRAALDPDAPVIRTDLDGKTDN
                     HIFDWETGDAAATEAVFAKADVVVKQEIVYPRVHPAPMETCGAVADLDPVTGKLTLWT
                     TSQAPHAHRTLYALVAGLPEHKIRVISPDIGGGFGNKVPIYPGYVCAIVGSLVLGKPV
                     KWMEDRSENLTSTSFARDYIMVGEIAATSDGKILAIRSNVLADHGAFNGQAAPTKYPA
                     GFFGVFTGSYDIEAAYCHMTAVYTNKAPGGVAYACSFRITEAVYFVERLVDCLAFDLR
                     MDPAELRLRNLLRPDQFPYRSKTGWVYDSGDYEATMRMAMDMIGYDALRAEQKERRAR
                     GELMGIGMSFFTEAVGAGPRKDMDILGLGMADGCELRVHPTGKAVVRLSVQTQGQGHE
                     TTFAQIVAEELGIPPDDIEVVHGDTDQTPFGLGTYGSRSTPVSGGAAALVARKVRDKA
                     KIIASGMLEVSVADLEWEKGSFHVKGDPSASVTIQDIAMRAHGAGDLPEGIEGGLDAE
                     VCYNPENLTYPYGAYFCVVDVDPGTAVVKVRRFLAVDDCGTRINPMIIEGQVHGGIVD
                     GIGMALMEMIAFDDDGNCLGGSLMDYLIPTALEVPHLETGHTVTPSPHHPIGAKGIGE
                     SATVGSPPAVVNAVVDALAPFGVRHADMPLTPSRVWEAMQGRARPPI"
BASE COUNT          863 a         1847 c         1782 g          878 t
ORIGIN      
        1 atgggcagca ccggcgcggg gcctgttgcg ccgtcgggcg aggtcgtgtc aaccggcccg
       61 aacaccgagg tgaagacggc gtcgaaggcg ctcagatctt ccatccggtt gaccagggtc
      121 agcctggccg cccaatagag cgccggcgtg gtgccgggca ccagcagtcg cagcgcctgg
      181 acgaaagtgg cctggccatt ggcggacacc agcactccaa cgccgcgcag ccgcaccacc
      241 agtgcggctg cgaacgccgc caagtcgacg ccccgcaaca tccatggcgt attcacgaag
      301 cgctaacgtc tccggcgcag cacccagatc aataccagcg ccgcgagagc cgccgccgcg
      361 gcggcgccgt acttcttgag gcggtcgccg ccggcgagct gcagcaggtc gatgggctcg
      421 ggctcggccg cgggggcacg gcgcggcgcc gggacgcgct cggtctcggc gggactctcg
      481 ggaggtggtg gagtctccgt attcttcgcc accagttcgg cttccagcga ttccacaaac
      541 tgacccagca acttctccga cacctgctgc agcattccgc tgccgaactg ggccagcttc
      601 ccgacgatct tcaggtcggt gtcgacggtg acattggtgt gctccccgtc cgcgttcagc
      661 agggcggtga tcgtggcggc cgcgttgccg gtgccccgcg attccttgcc cttggcgtcg
      721 atgaccgccc ggtgctgatc gcggtcctgt tcgacgaacc gcaccttgcc gctgaattcg
      781 ctggtgaccg gcccgacctt gaccttgacc ctgccgaggt agtcgtcgcc ctcatggccg
      841 gtcagctgcg cgcccggcat cagcgggatc acctgctcca tgtcgcacaa cacatcccag
      901 gcctgctcga tgggcgcgct gacggtgaac tggttggcga tcttcatgct gtgtgtcctt
      961 tcacgtgcgg ctgcactcag taaaagtctg gtacgcatcg cgaatcaggg tgcggtcgtc
     1021 cggcgtcttg gccagcgccc ctaggctggc cagtgcggcg gggtcgacca ggtcggcgac
     1081 gccgagcgac accagggccg ccacccagtc gatggcctcg gccacgccgg gaggcttgtc
     1141 cagatccaga tcgcgtgtcg cgccgacgaa ttgggtggcg tgctcgatca ggggcgccgt
     1201 cgcccccggc accgtgcgtc ggacaatggc ggcggcgcgg accgggtccg gatagtcgat
     1261 ccagtgatac aggcagcggc gtcgcaaggc atcgtgcaga tcgcggctgc ggttggaggt
     1321 cagcaccgcg atcgggggat gctcggcgac aaaggtgccg agctcgggga ccgtgaccgc
     1381 ggattcaccg aggaactcca gcagcaacgc ctcgaattcg tcgtcggccc ggtcgatttc
     1441 gtcaatcagc agcaccgggg ccctcgggcc gcgatgccgg acgcaccgca ggatgggccg
     1501 atccaccaga tacctctcgg tgtagagatc ggcttccgag atatcggaga cgcccgcgcc
     1561 acgggcttcg gccagccgga tggacagcaa ctggcgctgg tagttccagt catacagcgc
     1621 ctcgttcgcg gtcaatcctt catagcactg cagccggacc agcggggtat tcagcaccgc
     1681 cgcaagggtt ttagctgccg tcgtcttgcc gaccccgggc tcgccctcca acagcagcgg
     1741 ccggccgagc gtcaccgcca gatagatggc cgacgcggtc cccgtgtcga gcaggtagtc
     1801 gtgtgcatcg aaccgggtga tcacgtcgtc gacatcgccg aacgtcactg tgcccccatc
     1861 aggactccac cagtcggcgg tagtcgtccc aggtgtccac gtcgagcggg accgggccgg
     1921 ccacctcgag ttcgcgcacc ggatggcggc ccgactcgac gagtttccag acgcccttgt
     1981 cgccgtggag ttgacgaagg tcgccgaaca cgctccggcc caaccagaat gggtgcccga
     2041 tgccgtcgtc gtaacggcac accatgatgc cggccggcgc cgcacggccg tgttggatca
     2101 ttcgccgcac ggtggcgggc tctacgcacg gttgatcgcc gagcatcaac acgattccag
     2161 cggcccgccg gtccacgcga tcgagtgccg cgcgcagcga ggccgaacac ccggtcgccg
     2221 gctcgtcgac ggctatcacg tccgcgccgt cgagtcgcac cgcgtcgcgc accgctggtg
     2281 cggcgccacc gagggtgacg atgagttggt cgaacccgac gctgcgggcg acctccaggg
     2341 tcgcaccgag aagggtcgta tcccgatatg gcagaacctg tttgggtgtg cccagcctgc
     2401 gggaactgcc ggcggccagc acgacaccgg tgaccggcgg cgcagtcatg cgccgacggg
     2461 ccggtttgcg aacgccgtcc ggcatcccgg gccgcagaac cagtagtccg ctccggccag
     2521 tttcaggtgg tcggtggcgg gcccgaccgt caccgtcatg ccgcacaccg gatcgactgc
     2581 ctccggcggg ctgtccggaa gcacgaaggc gcggctcagg ctcggacctc cggcgccgcg
     2641 gatgccggcg acgagttcgg cggcgatcga cacggcgatc tccgccggag tcttggcgcc
     2701 gatgggcaat ccgaccgggg tgtgtacgcg gacccgctcg gcctcgcaga gttcgagttc
     2761 gttcaagatc gaggcaccac gaaccttgct ggccaccaag ccgatatacc cgacaccgtt
     2821 gtccagtgcg gcgcggatga tctcggcctc cgggccgccg tggctggcaa tcacaatggc
     2881 agttgccggg gcagttggcg gggcggcggc gtcggccagc tcggtgtccc gccggacgtc
     2941 ataaccgagt acctcacaga gttgggccag ggcatcgccg atgggtgtgc ccccgtggat
     3001 ctcgatcaac ggggccggca gctgcggcgc caggaagatc tccaacgacc ctccggacag
     3061 gcatgggttg accaccacgc acgcacccgg ggcctcagga aaatgcacgt cgccgtcggg
     3121 aagcacccgc agcagcacac tttcgcccgc ctgcagcgcg cccagcgccg ccttgcggac
     3181 ggagttctgc gcgcactgac cacccacgaa accctcgatt gtgccgtccg ccaacaggat
     3241 tgcttcgtca cccgggtacg ccgaggtggg cggctgagcc cgcaccaccg tcgcgtgcac
     3301 aaacggtgta cgcgcggcta gtagttgctg agccctctca ctaatggtcg gcactatatt
     3361 ggcggcctcg ctcgaccctg catggcttcc cagacccgcg acggtgtcaa cggcatatcc
     3421 gcgtgacgaa ccccgaacgg cgccaacgca tccaccaccg cgttcaccac ggcgggcggc
     3481 gatccgacgg tggccgactc accgatgccc ttggcgccga tcgggtgatg cggcgacggt
     3541 gtcacggtgt gaccggtctc cagatgcggc acctccagcg cggtggggat caggtagtcc
     3601 atcagcgatc cgcccaggca gttgccgtcg tcgtcgaacg cgatcatctc catcaacgcc
     3661 attccgatgc cgtcgacgat gccgccgtgc acctggccct cgatgatcat cggattgatg
     3721 cgggtgccgc agtcgtcgac cgcgaggaac cgccgcacct tgaccaccgc ggtgcccgga
     3781 tcgacgtcca ccacacagaa atacgcgccg tagggataag tgaggttctc cgggttgtaa
     3841 cacacctcgg catccaggcc accctcgatg ccctcgggca gatcgcccgc gccgtgggcg
     3901 cgcatcgcga tgtcctggat ggtcaccgac gccgacgggt cgcccttgac gtggaacgag
     3961 cccttctccc attccaagtc ggcgaccgaa acctcgagca tgcccgacgc gatgatcttc
     4021 gccttgtcgc ggaccttgcg ggcgaccagc gccgcggcac cgccggacac gggggtcgac
     4081 cggctgccgt aggtgcccag cccgaacggg gtctggtcgg tgtcgccgtg caccacctcg
     4141 atgtcgtcgg gcgggatgcc cagctcctcg gcgacgatct gggcgaacgt cgtctcgtgg
     4201 ccctggccct gggtctgaac cgacagtcgc acaacggctt tgcccgtcgg gtgcacgcgc
     4261 agctcacaac cgtcggccat gccgaggccg aggatgtcca tgtccttgcg cgggccggcg
     4321 cccacggcct cggtgaagaa ggacatgccg atgcccatca gctcgccgcg cgcccgtcgc
     4381 tccttttgtt cggcgcgcag ggcgtcgtag ccgatcatgt ccatggccat gcgcatggtg
     4441 gcctcgtagt caccggagtc gtacacccag ccggtcttgc tccgatacgg aaactgatcg
     4501 ggccgcaaca ggtttcgtaa ccgcagctcg gccggatcca tcctcaggtc gaaggccagg
     4561 cagtccacca ggcgctccac gaagtacacc gcctcggtga tgcggaacga gcacgcgtag
     4621 gccaccccac cgggcgcctt gttggtgtac accgcggtca tgtggcagta cgcggcctcg
     4681 atgtcgtagc tgccggtgaa cactccgaag aagcccgccg ggtatttcgt cggcgccgcc
     4741 tggccgttga acgcgccgtg atcggccagc acattggagc ggatcgcgag gatcttgccg
     4801 tcgctcgtgg cggcgatctc gccgaccatg atgtagtcgc gggcgaaact ggtggatgtc
     4861 aggttctcgc tgcggtcttc catccacttc accggcttgc ccagcaccag cgagccgacg
     4921 atcgcgcaca cgtaaccggg atagatcggt accttgttgc cgaagccgcc gccgatgtcg
     4981 ggtgagatca cccggatctt gtgttcgggc agacccgcga ccagtgcgta cagggtgcgg
     5041 tgtgcgtgcg gggcctggga ggtggtccac agcgtcagct tgccggtgac ggggtccaaa
     5101 tcggctaccg cgccgcaggt ttccatcggc gcggggtgta cgcgcggata cacgatctcc
     5161 tgcttgacca cgacgtcggc cttggcgaac acggcctcgg tggcggccgc atcaccggtt
     5221 tcccagtcga agatgtggtt gtcggtcttg ccgtcgaggt cggtccggat caccggcgcg
     5281 tctggatcga gcgccgcgcg gacatctatg accgggtcca gcgggtcgta gtcgacgtcg
     5341 atcagctcca gcgcatcccg cgccgaatac
//
LOCUS       DASSGQ010000278         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_293562,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000278 DASSGQ010000000
VERSION     DASSGQ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293562"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VFI55_12210"
     CDS             complement(<1..537)
                     /locus_tag="VFI55_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020731414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7074832.1"
                     /translation="MWEFETDPEYQAKLDWVEKFMVDELEPLDLVALDPYDKQNAEMM
                     AILRPLQQQVKDQGLWAAHLRPELGGQGFGQVKLALLNEILGRSRWAPSVFGCQAPDS
                     GNAEILALFGTDEQKARYLQPLLDGEITSCYSMTEPQGGSDPGQFVTAATRDGDYWVI
                     NGEKWFSTNAKHASFFIVM"
     gene            complement(708..1142)
                     /locus_tag="VFI55_12215"
     CDS             complement(708..1142)
                     /locus_tag="VFI55_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7074833.1"
                     /translation="MLKIASAHLWVHDQDVALKYWTEMVGMEVRQDVSLPELHIRWLT
                     VGPPGQDDVSIVLMAIPGPPVMDDETRNQVSEVMAKGFAGTVFLTTDDCQTAYETMRS
                     RGVEFTEEPHEMPYGIDCGFRDPSGNSVRLTQLAGIGLNTGE"
     gene            complement(1153..1605)
                     /locus_tag="VFI55_12220"
     CDS             complement(1153..1605)
                     /locus_tag="VFI55_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET7074834.1"
                     /translation="MPQVPPARYLLRAKDLADARYAEPITVDDMASAAGLSRAHFSRM
                     FSRTFGESPRSYLQTRRLERAAALLRYTERSVAEITVMVGLQSVGSFTTSFARVYGLP
                     PAAYRASLPPASVHARVPSCILRRDTRPPADSRVKTAHGKKTGDTGGP"
     gene            complement(1680..>2234)
                     /locus_tag="VFI55_12225"
     CDS             complement(1680..>2234)
                     /locus_tag="VFI55_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HET7074835.1"
                     /translation="NGGAGGLSGAPSLSGFGVALGGAGGAGGAANGAGGVAGAGGAGG
                     LGAALAGIGGGGHGGVGGSAATGTGGAGGAGGAGAGFVAGVGGAGGHGGSVTTGAGGN
                     GGDGGGGLGFLGGGGNGGDAGNGTLAANGGKGGDAGITLNGSETLSLLGDGGNGGNGV
                     NGGSGGTGGSAGMYAGTRGSNGTS"
BASE COUNT          359 a          771 c          706 g          398 t
ORIGIN      
        1 catgacgatg aagaacgagg cgtgcttggc gttggtggaa aaccacttct cgccgttgat
       61 cacccagtaa tcaccgtccc gggtcgcggc ggtgacgaac tgccccgggt cagacccgcc
      121 ctggggttcg gtcatcgaat aacacgaggt gatctcgccg tcgagcagcg gctgcaggta
      181 gcgggccttc tgctcgtcgg tgccgaacag cgcgagaatc tcggcgttgc ccgagtcggg
      241 tgcctggcag ccgaacacgg acggcgccca acgggagcgc ccgaggatct cgttgagcaa
      301 ggcgagcttg acctgaccaa agccctggcc gccgagctcg ggccgcagat gcgcggccca
      361 cagtccttgg tctttgacct gctgctgcag cggtcgcagg atcgccatca tctcggcgtt
      421 ctgtttgtcg tacgggtcga gcgcgaccag atcgagtggt tcgagttcgt cgaccatgaa
      481 cttttcgacc cagtccagct tcgcctggta ttccgggtcc gtttcgaatt cccacaccgt
      541 cggccacctt gccccagtgc agcgtcgcac cggactagtt gatgaagcca ccccatccta
      601 gatgcgttcg accggttgtc cgggaaggtt gtcgttgcgt gcagtgaatt ggtgtcagga
      661 gcggcatcgt tatcgccgaa tgcacccagc ccaaatattg caaatattca ttcgccggta
      721 ttcaggccaa ttccggcaag ttgggtcaac cggacactgt taccggatgg gtcgcggaaa
      781 ccacagtcga tgccgtacgg catctcgtgc ggttcctcgg tgaattccac gcctctggac
      841 ctcatcgtct cgtaggcggt ctggcagtcg tcggtagtga ggaacaccgt gccggcgaaa
      901 cccttcgcca tcacctcgct cacctggttg cgcgtctcgt catccatcac cggcggacct
      961 ggaatcgcca tcagcacgat ggacacgtcg tcctggcccg gtgggccaac cgtgagccat
     1021 cggatgtgta gctcaggcaa cgaaacatct tgtcgcactt ccatacccac catttcggtc
     1081 cagtatttca gtgcgacgtc ctggtcatgc acccacaaat gcgcgctggc tatcttcaac
     1141 atgggtacga cgctacggtc cgccggtgtc gcccgtcttc ttcccgtgtg ctgtcttgac
     1201 gcggctgtcg gcgggcggtc gggtatcccg ccgcaggatg cagctgggca cccgcgcatg
     1261 caccgaggcg ggcgggaggc tggctcggta tgcagcaggt ggtaacccgt acacgcgggc
     1321 gaagctggtg gtgaacgatc cgacactctg cagaccgacc atgacggtga tctcggcgac
     1381 ggaccgctcg gtgtagcgca gcagcgcggc cgctcgttcc aacctgcgtg tctgcagata
     1441 ggagcgcggt gattcaccga aggtccgact gaacatgcgg ctgaagtggg cgcgcgacag
     1501 cccggctgca gaggccatgt cgtccaccgt gattggttcg gcgtagcgcg cgtcggccag
     1561 gtctttggcg cgcagcaaat aacgagccgg cggaacttgc ggcataggtt ccagtatccg
     1621 gcggaccgtt ggactttgaa acccagcgtg gttatcggtg gcgctggacc ctcgtcgagc
     1681 tatgaagtcc cgttcgatcc ccgggtaccg gcgtacatcc cggcactgcc gcccgtgccg
     1741 ccgctcccgc cgttgacgcc attgcccccg ttgccgccgt cgccaagcag cgacagggtt
     1801 tcggacccgt tgagggttat ccccgcgtcg ccgcctttgc cgccattggc ggccagggtg
     1861 ccattgccgg cgtcgccgcc attcccgccg ccgccaagga agcctagacc gccgccgccg
     1921 tcgccgccgt tgccgccggc tccagtggta acgctgccgc catgcccgcc ggcgccgccc
     1981 acaccggcga caaacccggc accggcgccg ccggcgccgc cggccccgcc agtgccggtg
     2041 gccgcgctgc cgccaacgcc accgtgaccg ccgccaccga tgcccgcgag cgcggcaccg
     2101 agaccacctg cgccgccggc accggctacg ccgcccgcac cgttggcggc cccaccggcg
     2161 ccgccggcgc cgccaagtgc gacgccgaac ccgctgaggc tgggggcgcc gctcagaccg
     2221 ccggcaccgc cgtt
//
LOCUS       DASSGQ010000279         8456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1326540,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000279 DASSGQ010000000
VERSION     DASSGQ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8456
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326540"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..153
                     /locus_tag="VFI55_12230"
     CDS             <1..153
                     /locus_tag="VFI55_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET7074836.1"
                     /translation="TLTARGWVREGTVAAVERAAGGAALTWPAGWSVWPQRVYGDTRL
                     ELAERP"
     gene            307..789
                     /gene="coaD"
                     /locus_tag="VFI55_12235"
     CDS             307..789
                     /gene="coaD"
                     /locus_tag="VFI55_12235"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET7074837.1"
                     /translation="MSGAVCPGSFDPVTLGHVDVFERASVQFDEVVVAILTNPAKKGM
                     FDLDERIAMIEESTTHLPNLRVEAGRGLVVDFVRTLGMTAIVKGLRTGTDFEYELQMA
                     QMNRHVAGVDTFFVATAPRYSFVSSSLAKEVAMFGGDVSELLPEPVNRRLREKLSGRG
                     "
     gene            complement(811..1788)
                     /gene="purU"
                     /locus_tag="VFI55_12240"
     CDS             complement(811..1788)
                     /gene="purU"
                     /locus_tag="VFI55_12240"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HET7074838.1"
                     /translation="MPLAGLSWPRREPTGEGAMTTRSAEPQPDNYPPAPGGPPPPADI
                     GRLLLRCHDRPGLIAAVSNFLAQSGANIISLDQHSTAPEDGTFLQRAIFHLPGLTAAI
                     DGLEREFAATVAHRFTIDYRFTEAAKPKRVAIMASKDDHCLLDLLWRNRRGELEMSVV
                     MVIANHPNLAEHVRPFGVPFIHIPATRDTRAEAEQRQLQLLSGNVDLVVLARYMQILS
                     PSFLEAVGCPLINIHHSFLPAFVGAAPYKRARERGVKLVGATAHYVTEVLDEGPIIEQ
                     DVVRVDHTHTVDDLVRVGADVERAVLSRAVLWHCQDRVIVHDNETIVFR"
     gene            complement(1779..2990)
                     /locus_tag="VFI55_12245"
     CDS             complement(1779..2990)
                     /locus_tag="VFI55_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET7074839.1"
                     /translation="MRNPAEDRVTDRALRAIGHALALAGSMTWEILWALVLGFALSAV
                     VQAVVRRSTIVTLMGDDRPRTLAVAAGLGAASSSCSYAAVALARSLFRKGANFTAAMA
                     FEIGSTNLVVELGIILALLMGWQFTAAEFVGGPLMIVVLAVLFRLFVRSRLVDAAREQ
                     AERGIAGSMEGHAAMDMSIQKEGSFFQRLISPQGFTSVSHVFVMEWLAILRDLVLGLL
                     IAGAIAAWVPEKFWQIFFLADHPGWSALWGPIVGPIVAIVSFVCSIGNVPLAAVLWNG
                     GISFGGVIAFIFADLIILPILNIYRKYYGTAMMLTLLGTFYASMVAAGYLIEFLFGTT
                     HLIPTQRNATVMQAAISWNYTTWLNIAFLLVAAILVARFVTTGGIPMARMMGGAPDAG
                     HDHHDQTHECH"
     gene            3344..>8456
                     /gene="pks2"
                     /locus_tag="VFI55_12250"
     CDS             3344..>8456
                     /gene="pks2"
                     /locus_tag="VFI55_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015303076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolipid-1 biosynthesis
                     phthioceranic/hydroxyphthioceranic acid synthase"
                     /protein_id="HET7074840.1"
                     /translation="MESRVTPIAVIGMGCRLPGGIDSPDAFWQALLRGDDLVTEIPAD
                     RWDVDEHYDPERGVPGRSVSRWGGFVDDIGGFDAPFFGFGEPEATAIDPQHRLLLETS
                     WEAIEHAGIVPPSLAGSRTGVFVALAHDDYTVVTSDAGALAGPYGYTGTAFSMASGRI
                     SYALGLQGPAITVDTACSSGLLAIHMACRSLHQGETDLALAGGCMLMLEPPVSASMSA
                     QGMLSPTGRCRSFDVAADGFVRSEGCATVLLKRLPDALRDGDRILAVVSGTAANQDGR
                     SETITMPSSDAQAAVYRTALEVAGVDPATVSMVEAHGTGTPVGDPLEFTSLAEVYGAG
                     GNRVALGSGKSNVGHTESAAGAVGLIKAILGLRHGVVPPMLHFTGLPDELARIETGLF
                     VPEVVTPWPTNGAGGPRRAAVSSFGMSGTNVHALLEQAPETAAREPAQYDGPLVFPLS
                     ATSSDELRRTARRLAEWVQKNVEKNNDGCENSLFGDLAYTLTRRRAHRPVRTAVIAEN
                     LEDLVAGLHEIADAQTPYSAAAGQDDLGPVWIFSGQGSQWAAMGVDLLASERVFAETV
                     GVVEPLIARESGFSVTEAMMAPETVTGIDRVQPTLFAMQVALAATMKAYGVRPGAVIG
                     HSLGEVAAAVVAGALSLEDGVRVICRRSRLCARIAGSSAMASVELPADKVREELTARG
                     VTDVVVAVVASPQSTVIGGATQTVRDLVAAWEQREVMAREVAVDLASHTPQVDPILVE
                     LAETLADLNSMTPDVPFYSTTRLDPTERPVCDAGYWVDNLRQTVRFAAAVRAALEDGH
                     RVFAELAPHPLLTRAVEQTAGDANSPAQALASVRREQPLPHGLRGFLADLYSAGAAVD
                     FAALCPSGRLIEAPLPTWTHRRLLVGSSDQGSQPRGSRTLSVHPLLGAHVRLPEEPER
                     HAWQGEIGIAALPWLADHQVNGVAVFPGAGFCEMALASAAEVLGPDSEVRDIRFEQML
                     MLDDETSVSAVASVEDAGVVDFVVQTDREGERTRRAAAMLHIANDEQPQRRDVSALLA
                     THPQVAQGAALRQSFDERGIQYGPAFTALVSARTAEGKGRSVLAEVQLPSGVRSQQAG
                     YQVHPALLDACFQSVIAHPTVKGAADGVLLPLSVRRLRRYGPTRGTRYCHLRVTAERG
                     STFEADLDLLDESGAVLLAVEGLRMGASGGSGADHLLAERLLTIDWQQQTLPAVPERA
                     VGSWLLIDTAESDLLESRLTDAVKSLGAQCTTLDWPEEADHRAKTDLLGAQVRSGLEG
                     LVILCPPPAGEPDGEGLLRGREMVRHVVRMVRELPDLSAEPPRLYVVTRRAQIVLPDD
                     RPNLVQAGLRGLLRVIGAENPQLRVSQIDLDDDGDVEHVAQELLSGSAEDETAWRGGH
                     WYTARLRCTPLGPDERRLTTVDHASDRMRATVRHPGDLRTLEFIALDRKTPGPGEVEI
                     AVDASSLNFLDVLAALGRYPDLEGRPHQLGFDLGGVVTRVGADVTEVKVDDRVGGFSG
                     YANGSWGTFVTCDARLVTTLPANLTAAEAAATATGYGTAWYGLCDLARMTAEDKVLIH
                     SGTGGVGQAAIAIARLVGAQIYATAGSPERRQILRDMGIEHVYDSRSTEFGDQIRRDT
                     DGYGVDIVLNSLTGAAQRAGLKLLAHGGRFVEIGKRDIYGDTLLGLFPFRRNLTFYAV
                     DLALLTETHPGRLRELLRTVYQRVADGDLPMPVIAE"
BASE COUNT         1372 a         2667 c         2962 g         1455 t
ORIGIN      
        1 accttgactg cccgcggctg ggtgcgcgag ggaaccgtcg cggcggtgga gcgtgcggcc
       61 ggcggcgcgg cgctgacctg gcccgcgggc tggtcggtgt ggccgcagcg ggtttacggc
      121 gacacccgtt tggagctggc cgaacggccc tgaagcagcg tgtaccgtca tcggtcatgt
      181 gcgccgctcc tcctcgtcgt ttcactccac ctcgtcgccg gcgcgggtca tgagcgccgc
      241 tccacctcgt cgcttcgtcc ctcgcaagcg ggcggtagac ccaccgcatc gtcgccggcg
      301 cgcgtcatga gcggtgcggt gtgcccgggg tcgttcgacc cggtgacgtt aggccacgtg
      361 gacgtgttcg aacgcgcctc ggtgcagttc gacgaggtgg tggtggcgat cctgaccaac
      421 cccgccaaaa agggcatgtt cgacctcgac gagcggatcg cgatgatcga agagtcgacg
      481 acgcacctgc ccaacctgcg ggtggaggcc gggcgaggcc tggtggtcga cttcgtcagg
      541 acgctgggga tgaccgccat cgtgaagggc ctgcgcaccg gcaccgattt cgaatacgag
      601 ctgcagatgg cgcagatgaa caggcacgtg gccggcgtcg acaccttttt cgtggcgacc
      661 gcgccgcggt attcgttcgt gtcgtcgtcg ctggccaagg aggtcgcgat gttcggcggc
      721 gacgtgtcgg agctgcttcc cgaaccggtg aaccgccggt tgcgcgagaa gttgtccggc
      781 cgcgggtagt tcagccgcgg cgccgtgagg ctaccggaaa acgatggtct cgttgtcgtg
      841 cacgatgacg cggtcctggc agtgccagag cacggcgcgg gacagcaccg cgcgttccac
      901 gtcggcgccg acgcgcacca ggtcgtcgac ggtgtgggtg tggtcgacgc gcacgacatc
      961 ttgttcgatg atcggcccct cgtcgagcac ctcggtgacg taatgggctg tcgcgccgac
     1021 cagtttgaca ccccgttcgc gcgcccgctt gtagggggct gcgccgacga aggccggcag
     1081 gaacgaatgg tgaatgttga tcagtgggca gccgaccgct tcgagaaacg acgggctgag
     1141 tatctgcatg tagcgggcca gcaccaccaa atccacattg ccgctgagta actgaagctg
     1201 acgctgttcg gcttcggcac gggtgtcgcg ggtggcaggg atgtgtatga acggcacacc
     1261 gaagggcctt acgtgctcgg ccaggttggg atgattggcg atcaccatga caacagacat
     1321 ctcgagttcg ccgcgacggt tgcgccacaa cagatctaac aagcagtggt catccttgga
     1381 cgccatgatg gctacccgct tgggcttggc ggcctcggtg aaccggtagt cgatagtgaa
     1441 tctgtgggcc accgtggcgg cgaactctcg ctccaggcca tcgatggcag cggtcaggcc
     1501 gggcagatga aagatcgcgc gctgcaggaa tgttccgtcc tccggggcgg tggagtgctg
     1561 gtccagggaa atgatgttgg ccccggattg agccaggaag ttgctcaccg cggcgatcag
     1621 tcccggtcgg tcgtggcagc ggagcagcag ccgaccgata tcggccggcg gtggtggacc
     1681 gccgggcgct ggcggatagt tgtcaggctg gggctccgcg gagcgcgtgg tcatggcgcc
     1741 ttcaccggta ggttctcgtc tgggccagga tagccccgct agtggcattc atgagtctga
     1801 tcgtggtgat cgtgccccgc gtcgggggct ccgcccatca tgcgggccat ggggattccg
     1861 cccgtggtga cgaacctggc gaccagtatt gccgcgacga gcaggaaggc aatgttgagc
     1921 caggtggtgt aattccacga aatcgccgcc tgcatcaccg tggcgttgcg ctgggtcggg
     1981 atgagatgtg ttgtgccgaa caagaattcg atgagataac cggcggcgac catggacgcg
     2041 taaaaggtgc ccagcagcgt cagcatcatc gcagtgccgt agtacttccg gtagatgttc
     2101 aggatcggca ggatgatcag gtcggcgaag atgaaagcga tgacaccgcc aaagctgatg
     2161 cccccgttcc acagcaccgc agccagtggc acattgccga tcgagcagac gaacgacacg
     2221 atcgccacga tgggtcccac gatcggcccc cacagcgccg accagcccgg gtgatcggcc
     2281 aaaaagaaaa tctgccagaa cttttcgggc acccaggcgg cgatggcgcc ggcgatcaac
     2341 aggcccagaa cgaggtctcg cagaatcgcc agccactcca tgacgaacac gtgcgagacc
     2401 gacgtgaagc cctgcgggga aatgagccgt tggaaaaacg agccttcctt ttggatggac
     2461 atgtccatgg ccgcatggcc ttccatcgag ccggcgattc cccgctcggc ctgctcgcgg
     2521 gcggcgtcga cgagccggga tcgcacgaac aatcggaaca ggaccgccag caccacgatc
     2581 atcaacgggc cgccgacgaa ctcggcggcg gtgaactgcc agcccatcag cagggccagg
     2641 atgatgccca actccaccac gaggttggtg gaaccaattt cgaacgccat cgcggcggtg
     2701 aagttggcgc ccttgcggaa cagtgaccgc gccagggcca cggccgcata cgagcacgac
     2761 gacgatgccg cacccagccc ggccgccacc gcgagggtgc gcggccgatc gtcgcccatc
     2821 aacgtcacga tcgtcgagcg acgcaccacg gcctgcacca ccgccgacag ggcgaagccc
     2881 aggaccagcg cccacaggat ctcccacgtc atcgaccccg ccagcgccaa tgcgtgccct
     2941 atcgctctta gcgctctgtc cgtcactcga tcctccgccg gattgcgtat ttcggcgatt
     3001 atcactcatg gttcggaccg gcgctcccgc gcaccgattt ataccatcgg ggggtataag
     3061 tcgagccttc ggcgctgttt ggcgctcttt cgccccaccg agcaagaccg ctggtcagcg
     3121 gctttagacc cggtgacgac cggagcaggg ttcgcccttt cccacgccac gcttatgtcg
     3181 ggggtctaag ctcgggtgtt cgagtgatgg gctccgcatg agtactcggg tttatctcgg
     3241 gtctgggggt tgcgagagcg acgttgcagg ggtgccccca attcaaaatt gccgatgatg
     3301 ccttcgggta tcgccggttg agcggagacg aggttgaacg ccgatggaat cacgtgtcac
     3361 tcccattgcg gtcatcggaa tgggatgccg gcttccgggc gggatcgatt cccccgacgc
     3421 gttctggcag gcgttgttac gtggtgacga cctggtgacc gagatcccgg ccgaccgctg
     3481 ggacgttgac gagcactatg accccgagcg cggggttccg ggccggtcgg tgtcgcggtg
     3541 gggcggcttc gtcgacgaca tcggcgggtt tgacgcccca ttcttcggct tcggggagcc
     3601 ggaagcgact gctatcgacc cgcagcaccg gttgttgttg gagacgtcct gggaagcgat
     3661 cgagcacgcc ggcatcgttc caccgagctt ggccggctca cgcacgggcg tgttcgtggc
     3721 gttggcccac gacgactaca cggtggtcac tagcgatgcg ggcgccctgg cgggcccgta
     3781 cgggtacacc ggcactgcct tcagcatggc ttccggccgg atctcgtatg cgctggggct
     3841 gcagggtccg gccatcacgg tggacacggc gtgttcttcc ggcctgctgg cgatacatat
     3901 ggcttgtcgc agcctgcacc agggcgagac ggacctcgcc ttggccggtg gttgcatgct
     3961 gatgctggaa ccgcccgtga gcgcgtcgat gtcagcgcag ggcatgctct ctcccacggg
     4021 tcggtgtcgg tcgttcgacg tcgcagccga cgggttcgta cgttccgagg gttgcgccac
     4081 ggtattgctg aagcgactgc ccgacgcgtt acgagacggc gaccggatcc tcgccgtggt
     4141 gagtgggacc gccgccaatc aggacggtcg ttccgaaacg atcacgatgc catcgtcgga
     4201 tgcccaggcc gcggtgtacc ggacggcgtt ggaagtggcc ggagtggacc cggctaccgt
     4261 cagcatggtc gaagcgcacg gcaccggaac cccggttggt gacccgctcg aattcacaag
     4321 tctggctgag gtatacggtg ccggcggtaa tcgcgttgcg ctcggatcgg gtaagtcgaa
     4381 tgtcggccac accgagtccg cagccggtgc ggtcgggctc atcaaggcca tcctcgggct
     4441 tcgtcacggt gtggtgccgc cgatgctgca cttcaccgga ctaccggacg agcttgcccg
     4501 gatcgaaacc gggctgttcg tgccggaggt ggtcaccccc tggcccacca atggtgccgg
     4561 tggcccacga cgggcagcgg tgtcgtcgtt cgggatgtca ggaacaaacg tgcacgcgct
     4621 gctcgagcaa gctcccgaga ccgctgcacg ggagccggct cagtacgacg ggccgttggt
     4681 gttcccgcta tcggccacct cgtccgacga gctgcgccgg acggcgcgcc gattggccga
     4741 gtgggtgcag aagaacgtgg agaagaataa cgacggctgc gagaattccc tcttcggaga
     4801 cctggcctac acgttgacgc ggcggcgcgc acaccgcccc gtgcgcacag cagtgatcgc
     4861 ggagaacctc gaggacctgg tcgccggact acacgagatt gccgacgctc aaactcccta
     4921 ttcggcggcg gcgggtcagg atgacctggg gccggtgtgg attttctcgg gccagggctc
     4981 gcaatgggcg gcgatgggcg ttgacctgct cgccagcgaa cgggtgttcg ccgagaccgt
     5041 cggggtggtc gagccgctga ttgcccgcga gtccgggttc tcggtgaccg aggcgatgat
     5101 ggcaccggag accgtgaccg gtatcgaccg ggtgcagccc accctgttcg ccatgcaggt
     5161 cgcgctggca gcaacgatga aggcgtacgg ggtccgtccg ggtgcggtca tcggccattc
     5221 gctgggcgaa gtggctgccg cggtcgtggc tggagcgctg tcgttggaag acggggtacg
     5281 cgtgatttgc cgccgctccc gcctgtgcgc ccgcatcgcg ggttccagcg cgatggcatc
     5341 ggtggagctg cccgccgaca aggtgcgcga ggaattgacg gcccgcggcg tcaccgatgt
     5401 cgtggtcgcg gttgtggcct cgccgcagtc caccgtgatt ggtggggcga cacagacggt
     5461 gcgtgacctg gtcgcggcgt gggagcagcg cgaggtgatg gcccgcgagg tggccgtcga
     5521 cttggcatcg cacaccccgc aagttgatcc gatcctcgtc gagctggccg agacattggc
     5581 ggacctgaac tcgatgacac cggacgttcc gttctactcc acgacccgtc ttgacccgac
     5641 tgagcggccg gtgtgcgatg caggctactg ggtcgacaat ttgcgtcaga cggtgcggtt
     5701 cgccgcggcg gtgcgcgccg cgcttgagga tggccaccgg gtcttcgcgg aactggcccc
     5761 acacccactg ctgacgcgcg cggtcgagca gaccgccggg gacgccaata gtcccgccca
     5821 ggcgctggcg tcggtgcggc gcgagcaacc gctgccccac gggctgcggg gctttttggc
     5881 ggatctgtac agcgccggcg ccgccgtgga tttcgcggcc ctgtgtccga gcgggcgcct
     5941 gatagaggcg ccgcttccga catggactca ccgccggctc ctcgtgggat ccagcgacca
     6001 aggctctcag ccacgaggct cccgaactct ctcggtgcac ccgctgctgg gcgcgcatgt
     6061 gcgactgccc gaggaaccgg agcgccacgc gtggcagggc gagatcggta tcgcggcgct
     6121 gccctggctg gcggaccacc aggtcaacgg ggtggcggtc ttccctggtg cgggcttctg
     6181 cgagatggcg ctcgcgtccg ccgccgaagt gctggggccg gactccgagg tgcgcgacat
     6241 tcgcttcgag cagatgctga tgctggatga cgagacttcg gtcagcgcgg tggcttccgt
     6301 tgaggatgcc ggcgtcgttg acttcgttgt gcagactgat cgcgaagggg agcgcacccg
     6361 gcgagcagcc gcgatgctgc acatcgccaa tgacgagcag ccacagcggc gagatgtttc
     6421 tgccttgctg gccacccacc cgcaggtggc gcagggcgcc gcgttgcggc aatcgttcga
     6481 tgagcgaggc atccagtacg gtccggcttt cactgctctg gtgtcggcgc gcactgccga
     6541 aggcaagggc cgcagcgtct tggccgaggt ccagctaccg agcggggtac ggtcacagca
     6601 ggccggttac caggtccatc ccgcactcct ggatgcctgc ttccagtcgg tgattgcgca
     6661 cccaaccgtc aagggcgctg ccgacggagt gctgttgccg ctgagtgtgc ggcggctgcg
     6721 tcgctacggc cccacgcgcg gcactcgcta ctgccatctg cgggtcactg cggagcgtgg
     6781 gtcgacgttc gaagcggatc tggatctgct ggacgagagc ggcgcggtgc ttctggctgt
     6841 ggagggcctg cggatgggcg ccagtggcgg cagcggcgct gaccacctgc tggccgagcg
     6901 gctgctgacc atcgattggc agcagcagac gttgccggcc gtacccgagc gggccgtcgg
     6961 gagttggctg ctgatcgaca ccgccgagtc tgatctcttg gaatcccggc ttaccgatgc
     7021 ggtgaaatct cttggcgcac aatgcacaac gctcgactgg ccggaggaag ccgaccatcg
     7081 ggccaagacc gacctgctgg gagctcaggt tcgcagtgga ctcgagggat tggtgatcct
     7141 ctgcccgccg cccgccggcg aaccggacgg tgagggtctg ctgcgcggcc gcgagatggt
     7201 gcgccacgtc gtgcgcatgg ttcgcgagct gccggacctc tccgcggagc cgccgcgact
     7261 gtacgttgtg accaggcggg cccagatcgt gctgcccgac gaccggccca acctggtgca
     7321 ggccggactg cgcggcctgc tgcgggtgat cggggcggag aatccacagc tgcgggtcag
     7381 ccagatcgac ttggacgacg acggtgacgt agagcacgtg gcgcaggagc tgctgtccgg
     7441 ttcggcagaa gacgagaccg cctggcgcgg tggccactgg tacacggcgc gactgcgctg
     7501 cacgccgctg gggcccgacg aacgccggct taccaccgtc gatcacgcat ccgaccggat
     7561 gcgcgcgacg gttcgtcatc ccggtgacct gcggactctg gaattcattg ccctggaccg
     7621 caagacgccg gggccggggg aggtcgagat cgcggtcgac gcgtcgagcc tcaacttcct
     7681 ggatgtcctc gccgccctgg gccgctaccc cgacctcgaa ggccggccac accagttggg
     7741 gttcgacctc ggcggcgtgg tgacgcgggt gggcgcagac gtcacggagg tgaaggtcga
     7801 tgaccgcgtc ggcgggttct ccggctacgc caacggcagc tggggcacgt tcgtcacctg
     7861 cgacgcccgg cttgtcacca ccctgccggc caacctgact gccgctgaag cagccgctac
     7921 tgcaaccggg tacgggacag cgtggtacgg gctgtgcgat ctggccagga tgacggccga
     7981 ggacaaggtg ctgatccatt cgggcacggg cggcgtcggc caggccgcga tcgcgatcgc
     8041 tcgcctggtc ggagcacaga tctacgcgac cgcgggcagc ccggaacgcc ggcagatctt
     8101 gcgcgacatg ggtattgagc atgtttacga ctcgcgcagc accgaatttg gcgaccagat
     8161 ccgccgcgac accgacggct acggcgtcga catcgtgctg aactcgctga ccggcgcggc
     8221 gcagcgcgcc gggctgaaac tgctggctca tggcgggcgg tttgtcgaga tcggcaagcg
     8281 cgacatctat ggcgacaccc tgctgggact gttcccgttc cgtcgcaatc tcacgttcta
     8341 tgccgtcgat ctggcgttgc tgacggagac ccatccgggc cgattgcggg agctgttgcg
     8401 gaccgtgtac cagcgggtcg ccgacggtga cctaccgatg ccggtcatcg ctgaat
//
LOCUS       DASSGQ010000280         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_749146,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000280 DASSGQ010000000
VERSION     DASSGQ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749146"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VFI55_12255"
     CDS             complement(<1..902)
                     /locus_tag="VFI55_12255"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET7074841.1"
                     /translation="MSASPVKVAVTGAAGQIGYSLLFRLASGSLLGPDRPIELRLLEI
                     EPALKALEGVVMELDDCAFPLLAGVQIGADPNKIFDGVNLALLVGARPRGPGMERGDL
                     LEANGAIFTAQGKALNSVAADDVRVGVTGNPANTNALIAMSNAPDIPKERFSALTRLD
                     HNRAISQLAKKTGASVTDIKKITIWGNHSATQYPDIFHAEVGGKNAAEVVNDQAWIEN
                     DFIPTVAKRGAAIIDARGASSAASAASATVDAARDWLLGSPADNWVSMAVVSDGSYGV
                     PEGLISSFPVTTKDGNWEIVGGLE"
     gene            1143..2243
                     /gene="corA"
                     /locus_tag="VFI55_12260"
     CDS             1143..2243
                     /gene="corA"
                     /locus_tag="VFI55_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HET7074842.1"
                     /translation="MFQGFDALPEALRPLAHEPHVSPEVPPHPPNQALIDCAVYAEGH
                     RLPGRPSYADALRKVREIELLGHQAFVWVGLREPDLTQMQDVADVFGLHPLAVEDAVC
                     AHQRPKLERYDDTVFLVLKTVTYVPHESVVLAREIVETGEIMIFVGKDFVVTVRHGEH
                     GGLSEVRKRLEADPEQLGLGPFAVMHAIADNVVDHYLEVSALIESDIDSIEEVAFAPS
                     RKLDVEPIYLLKREVVELRRCVSPLSVAFQRMQTENKDVISKEVRRYLRDVADHQSQA
                     ADQIGSYDDMLNSLVQAALARVGMQQNNDMRKMAAWAGILAVPTMVAAIYGMNFHFMP
                     ELNWTWGYPGVMAAMTVVCLVLYFQFRRNNWL"
     gene            complement(2448..>3011)
                     /locus_tag="VFI55_12265"
     CDS             complement(2448..>3011)
                     /locus_tag="VFI55_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HET7074843.1"
                     /translation="AHFGTEPDSASVTFLGAERIEVLRFRARANGPQWIVHYVSLGCS
                     RHPMTDPTKMVADPRRGPRAEIVLRLRDPGPVTGLARSLAVLAATPAVDGVVLEADAL
                     IDLGSPIWTSPTRRVPFTAVLLGDCDIPDLTLESPLEPVTFLSATPLTAIEAAWVRLK
                     GAEAMRQAWRNDGVDVLDPKRRAAQPD"
BASE COUNT          511 a         1008 c          988 g          504 t
ORIGIN      
        1 atctccaggc cgccgacgat ctcccagttg ccgtccttgg tggtcaccgg gaacgacgaa
       61 atcagccctt cgggcacacc gtaggaacca tcggagacga cggccatcga gacccagttg
      121 tcggcggggc tgccgagcaa ccagtcgcgg gcggcgtcga cggtcgccga cgcggccgag
      181 gcagccgacg aagcgccgcg cgcgtcgatg atcgccgccc cgcgcttggc gacggtgggg
      241 atgaagtcgt tctcgatcca cgcctggtcg ttgaccacct cggcggcgtt cttgccgccg
      301 acctcggcgt ggaagatgtc ggggtactgg gtggccgagt ggttgcccca gatcgtgatc
      361 ttcttgatgt cggtgaccga cgcgccggtc ttcttggcca gctgcgagat cgcccggttg
      421 tggtccaggc gggtcagcgc cgagaaccgc tccttcggga tgtcgggtgc gttgctcatc
      481 gcgatcagcg cgttggtgtt ggccgggttt ccggtcacgc cgacgcggac gtcgtcggcg
      541 gccaccgagt tgagcgcctt gccctgcgcc gtgaagatcg cgccgtttgc ctccagcagg
      601 tcgccgcgtt ccatgcccgg gccgcgcgga cgggcgccga cgagcagcgc caggttcacc
      661 ccgtcgaaga tcttgttcgg gtcggcgccg atctggacgc ccgccagcag cgggaaggcg
      721 cagtcatcga gctccatcac gacaccctcg agcgccttga gtgccggttc gatctccagc
      781 agacgcagct cgatcgggcg gtcgggcccc agcagcgacc cgctcgccag gcggaacaac
      841 aggctgtaac cgatctggcc ggcagcgccg gtgacggcga ccttgacagg acttgcgctc
      901 acgtcggtgt gctccttctg gagaaggtca ggatgtagga attgtcggtt cgggtcgaac
      961 tacgtcgaaa ctagcgcacc cctgccggcc tgaaacctcc ggtccaagac cagcatgttg
     1021 cccgcgagcc gcccggctac gtcatcgccc gtcgggccgt cgcgtactct tcatgaaccg
     1081 tctcgccgga acgcgtagca ttgagcaccg cttggccgga cctgccgaaa tcggaggctg
     1141 aggtgttcca gggattcgac gcattgcccg aagcgcttcg accgctcgcg cacgagccgc
     1201 atgtttcgcc ggaggttccc ccccacccgc ccaaccaggc cctgatcgac tgcgccgtct
     1261 acgcggaagg ccaccggcta cccgggaggc ccagctacgc cgacgcactg cgcaaagtgc
     1321 gcgagatcga actgctcggg caccaggcat tcgtctgggt gggcctgcgc gagccagacc
     1381 tgactcagat gcaggacgtg gcagacgttt tcgggctgca cccgttggcc gtcgaagacg
     1441 cggtatgcgc acatcagcga ccgaagctgg agcggtacga cgacacggtc ttccttgtcc
     1501 tcaagaccgt cacctacgtt ccgcacgaat cggtggtgct ggcccgcgag atcgtcgaaa
     1561 ccggcgagat catgatcttc gtcggcaagg atttcgtggt caccgtccgc cacggtgaac
     1621 acggcggact gtccgaggtg cgcaagcgcc tcgaagccga ccccgagcag ctgggccttg
     1681 gcccgttcgc ggtgatgcac gccatcgccg acaacgtggt ggaccactac ctcgaggtga
     1741 gcgctctgat cgaatccgat atcgacagca tcgaggaagt ggcgtttgcc ccgagccgca
     1801 agctcgacgt cgaaccgatt tatctgctca aacgcgaggt cgtcgaacta cggcggtgcg
     1861 tcagcccgct gtccgtcgca ttccaacgaa tgcagacgga aaacaaagac gtgatctcca
     1921 aggaagtgcg gcgctaccta cgcgacgtcg ccgaccacca gtcgcaggcc gccgatcaga
     1981 tcggcagcta cgacgacatg ctcaattcgt tggtacaggc ggcgttggcc cgtgtcggca
     2041 tgcagcagaa caacgacatg cgcaagatgg cggcctgggc cggcatcttg gcggtgccca
     2101 ccatggtcgc cgccatctac ggtatgaact tccatttcat gcccgagctg aactggacct
     2161 ggggttaccc cggggtgatg gccgcgatga ccgtcgtctg cctggtcctg tacttccagt
     2221 tccgccgcaa caattggctc tagcccggcg acgatgcagg ccgcacgcgg cctgagaagg
     2281 agccgggcaa tctgcctagc ccggcgacga tgcaggccgc acgcgggcag tgtgtgccct
     2341 gggcgtccgg tgtgcatcca gggtggtgat ttcgcgattc gcccgccctc cgcacacagt
     2401 caacgccgcc tgcgcacact cgatgaggct gcgttgcagt cggaacctca atcaggttgt
     2461 gcggcgcggc gtttcggatc gagcacgtcg acgccgtcgt tgcgccaggc ctgtcgcatc
     2521 gcctcggcgc ccttgaggcg cacccaagcc gcttcgatcg cggtgagtgg agtcgccgag
     2581 aggaacgtca ccggttcgag cggcgactcc agcgtcagat ccgggatatc gcagtcgccc
     2641 agcaacaccg ccgtgaacgg cacccgtctc gtcggagatg tccagatcgg cgagccgagg
     2701 tcgatcagcg cgtcggcttc cagcaccacg ccatcgacag ccggcgttgc cgccagcacc
     2761 gccaggctgc gggcaaggcc ggtgaccggt ccgggatctc gcaggcgcag aacaatttcg
     2821 gcgcgcgggc cacgccgggg gtcggcgacc atcttggtcg gatcggtcat cgggtgacgc
     2881 gagcacccca gggacacgta gtgcactatc cactgcggtc cgttcgcccg cgcgcgaaat
     2941 cgcagcacct cgatgcgctc ggcgcccaag aacgtcacgc tcgcggaatc cggctcggtg
     3001 ccgaagtggg c
//
LOCUS       DASSGQ010000281        18000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_263550,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000281 DASSGQ010000000
VERSION     DASSGQ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18000
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263550"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..94)
                     /locus_tag="VFI55_12270"
     CDS             complement(<1..94)
                     /locus_tag="VFI55_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7074844.1"
                     /translation="MDGTGDSAAAAARDLRVVFSRLRRRLRDLAI"
     gene            complement(138..443)
                     /locus_tag="VFI55_12275"
     CDS             complement(138..443)
                     /locus_tag="VFI55_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074845.1"
                     /translation="MRKRQIYLGVAGLALAAIGLAALWLPISLGQHDMYGFPIKCGNG
                     FKSDFSQVAESSDLTAKCGSALLTRRIWAIPLLAIGWLMEMAFLIYWVGAAPTRKTS"
     gene            complement(466..690)
                     /locus_tag="VFI55_12280"
     CDS             complement(466..690)
                     /locus_tag="VFI55_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074846.1"
                     /translation="MLDKLRQVLDFQLTIGQLIAIGLIIGTPYLLVGTVWSTTHTEHL
                     HQMHGIDLAVSFLGSIVSWPVLLFSNVCMT"
     gene            complement(721..1383)
                     /locus_tag="VFI55_12285"
     CDS             complement(721..1383)
                     /locus_tag="VFI55_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HET7074847.1"
                     /translation="MNLDHIAGVAHEPASTPARGVVVLTHGAGGSRESPLLQQVCDEW
                     ARRGWLAVRYNLPYRRRRPTGPPSGSAAADRAGIVEAIAVCRGLADGPMIVGGHSYGG
                     RQTSMVVSDKQAQVDVLTLFSYPLHPPGKPERARTEHLPDITVPTVFTYGTADPFGTP
                     TEIRDAARIVAAPIELVEITGARHDLRSKTLDVPALAVDAALRLLGAPGSQTGGQGSG
                     LK"
     gene            complement(1457..2308)
                     /gene="thiD"
                     /locus_tag="VFI55_12290"
     CDS             complement(1457..2308)
                     /gene="thiD"
                     /locus_tag="VFI55_12290"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET7074848.1"
                     /translation="MSYLPLVLPVPPPGTTPLRVLTIAGSDSGGGAGIQADMRSMALL
                     GVHACVAVTAVTVQDTLGVKSFHEVPDDVVAGQIEAVVTDIGIQSAKTGMLASSAIIA
                     AVAATWRRLGLTVPLVVDPVCASMHGDPLLAPEALDSLRNQLLPLATLVTPNLDEVRL
                     LVDIDVVDATSQRAAAKALHALGPQWALVKGGHLRSSDRSCDLLYGSAFGEADFHEFD
                     SPRLATGNDHGGGDTLAAAVACALAHGFSVPDAVGFGKRWVTECLRAAYPLGHGHGPV
                     SPLFRLS"
     gene            complement(2305..3933)
                     /gene="thiC"
                     /locus_tag="VFI55_12295"
     CDS             complement(2305..3933)
                     /gene="thiC"
                     /locus_tag="VFI55_12295"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HET7074849.1"
                     /translation="MTVTIEPSVTTGPIAGSSKAYRELDGGLCVPFRRVHLSTGDHFD
                     LYDTSGPYTDTEAVIDLAAGLPARPGVVRDRGSQLQRARAGEITAEMAYIAAREDMPA
                     DLVRDEVARGRAVIPANHNHPESEPMIIGKAFAVKVNANIGNSAVTSSIAEEVDKMVW
                     ATRWGADTIMDLSTGKNIHETREWILRNSPVPVGTVPIYQALEKVKGDPTELTWEIYR
                     ETVLEQCEQGVDYMTVHAGVLLRYVPLTAKRVTGIVSRGGSIMAAWCLAHHRESFLYT
                     NFEELCDIFARYDVTFSLGDGLRPGSIADANDAAQFAELRTLGELTKIAKAHGAQVMI
                     EGPGHVPMHKIVENVRLEEELCEEAPFYTLGPLATDIAPAYDHITSAIGAAIIAQAGT
                     AMLCYVTPKEHLGLPDRKDVKDGVIAYKIAAHAADLAKGHPRAQERDDALSTARFEFR
                     WNDQFALSLDPDTAREFHDETLPAEPAKTAHFCSMCGPKFCSMRITQDVRDYAAAHGL
                     DTEEAIEAAMQDGMEEKSREFAEHGNRVYLPITQ"
     regulatory      complement(3924..4033)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4222..4908
                     /locus_tag="VFI55_12300"
     CDS             4222..4908
                     /locus_tag="VFI55_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS and ANTAR domain-containing protein"
                     /protein_id="HET7074850.1"
                     /translation="MTPTGNSKLPPGATEAAGEYLNVGTFRFWFVGERWEWSDEVAAM
                     HGYEPGTVAPTTKLLLSHKHPDDRAHVKDLLDYALQSEESFSSRHRFIDTAGRVHDAI
                     VVADRMLDEAGAVLGTAGYYIDLTETFDEARYETRQEVLDEALPDLFENRAAIEQAKG
                     VLMYVYRVSADQAFRVLQWRSQQTNVKLRALAKQLLAEISTLMPPTAAVQSQFDHVLL
                     TVHERIPAES"
     gene            complement(4918..5193)
                     /locus_tag="VFI55_12305"
     CDS             complement(4918..5193)
                     /locus_tag="VFI55_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074851.1"
                     /translation="MAENKSRRATPQREAVEKIREAETFVVNVPLVGQMEIPRPEQLA
                     YYGGLAALAALELIDWPVALVIAAGHVLASNHHNRLLEELGEAMEEV"
     gene            5257..5436
                     /locus_tag="VFI55_12310"
     CDS             5257..5436
                     /locus_tag="VFI55_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv1535 domain-containing protein"
                     /protein_id="HET7074852.1"
                     /translation="MSTTGSVADPLVSSIASVLRVPLVELYALLWRVGVVEIRQTRGA
                     ELNEAHPAVRRRATG"
     gene            complement(5479..5919)
                     /locus_tag="VFI55_12315"
     CDS             complement(5479..5919)
                     /locus_tag="VFI55_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074853.1"
                     /translation="MSFVGGAVRNVGKSVGRAADATTAAAGAVGGAAVNGLVGGVTGA
                     AEGIKRGIGSGSHSTPAAALAIGALGVAGLVEWPLLVAVGGGALLLHKMQRKPQPPAP
                     AKANLKPVPSEPAKKAVAEKATSKPAVKKAPGRRAGATGLRNTN"
     gene            complement(6121..6927)
                     /locus_tag="VFI55_12320"
     CDS             complement(6121..6927)
                     /locus_tag="VFI55_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET7074854.1"
                     /translation="MRLATWNVNSIRTRLNRVLDWLARAEVDVLAMQETKCSDSQFPT
                     LPFFELGYEVAHVGVNQWNGVAIASRVGLDDVQIGFEGQPSWSSKPEVAATAEARALG
                     ATCGGIRVWSLYVPNGRALDDPHYTYKLNWLAALRDTASGWLRDDPAAPIALVGDWNI
                     APTDDDVWSTEFYAGATHVSEPERLAFNAIVDAQFTDAVRPFTPGPGVYTYWDYTQLR
                     FPKRQGMRIDFILASPALAGRVTDAQIVREERKGKAPSDHAPVLIDVSPV"
     gene            complement(6966..7877)
                     /locus_tag="VFI55_12325"
     CDS             complement(6966..7877)
                     /locus_tag="VFI55_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7074855.1"
                     /translation="MVSWPDLGTRVTVRYRRPAGSDPPLTDAVGHLLAVDPVVRVRTK
                     TGEVAECAPTDVVALRVLTDTPIRTSQIRALEHAAAMAWPGAEHTWLDGWLLRAGHGA
                     DFAANSALPLDISAHSGAIPEIVAWYDRRGLTPRLAIPDRLLLLPSGVPDEHTERVLV
                     RDVSAASIGEPDPSIALSARPGDTWLRLHREVPVAVLTAVIDGELIFGTHSDQAVARA
                     ALTVAPDGIRWVGLSQLHAVDDQSTPGAVAALCEALLAWGTRRGATRAYLRSGGQHGS
                     PTTLADSLAFRLHHQCRYVRARPMDSV"
     gene            complement(7877..8470)
                     /locus_tag="VFI55_12330"
     CDS             complement(7877..8470)
                     /locus_tag="VFI55_12330"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET7074856.1"
                     /translation="MAVVPIRIVGDPVLHTPTTPVAVAADGSLPADLAALISTMYDTM
                     DAAHGVGLAANQIGVGLRLFVYDCSEDRGLTDRRRGVVINPVLETSEIPETMPDPGND
                     DEGCLSVPGESFPTGRAKWARVTGLDADGRRVAIEGTGLFARMLQHETGHLDGFLYLD
                     RLIGRHARAAKRAVKSHGWGVPGLSWTPGEGPDPFGH"
     gene            8841..9149
                     /locus_tag="VFI55_12335"
     CDS             8841..9149
                     /locus_tag="VFI55_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HET7074857.1"
                     /translation="MDSAMARANRSGDDSEIADGLTRREHDILAFERQWWKFAGIKED
                     AIKELFSMSATRYYQVLNALVDRPEALAADPMLVKRLRRLRASRQKARAARRLGFEVT
                     "
     gene            9201..9680
                     /locus_tag="VFI55_12340"
     CDS             9201..9680
                     /locus_tag="VFI55_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HET7074858.1"
                     /translation="MKERVPDSTGLPLRAMVMVLLFLGVIFLLLGWQALSSSSNSDDD
                     SASPVSSVTSTTAPVSTSTSAKPPANDAEVRIYNISSKEGVAAHTRDDLAAAGFKVTD
                     VGNLSLPDITATTVYYTDADGEHATADAVGQKLGAPVEPRIPALSNQPPGVIVLVAG"
     gene            9778..10431
                     /locus_tag="VFI55_12345"
     CDS             9778..10431
                     /locus_tag="VFI55_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HET7074859.1"
                     /translation="MLKLSPLLLGSCVVLASACSSPQHASTTPGTTPAIWTGSSAPSG
                     PSGTEGGQQPVAQAVITHLRAPDGTQIATAKFDFGNGYATVTIATTGVGQLTPGVHAV
                     HIHKVGKCEPNSVAPTGGAPGDFLSAGEHFQAPGHAAEPASGDLTPLEVRKDGSGMMV
                     TTTDAFAMDDLLSGEKTAIIIHAGADNAPGGMPGPDGMAMGTGDASKRVACGVVGAG"
     gene            10421..11551
                     /locus_tag="VFI55_12350"
     CDS             10421..11551
                     /locus_tag="VFI55_12350"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HET7074860.1"
                     /translation="MPASGAKTRIDFAPSPEGTIGVEWEFALVDAQTRDLSNEATAVI
                     AEIGENPRVHKELLRNTVEVVSGICECTAEAMEDLRQTLRPARQVVRDRGMELFCAGT
                     HPFARWSAQKLTDAPRYAELIKRTQWWGRQMLIWGVHVHVGISSANKVMPIMTSLLNY
                     YPHLLALSASSPWWGGEDTGYASNRAMMFQQLPTAGLPFHFQTWAEFEGFVYDQKKTG
                     IIDHMDEIRWDIRPSPHLGTLEVRVCDGVSNLRELGALVALTHCLVVDLDRRLDAGET
                     LPTMPPWHVQENKWRAARYGLDAVIILDADSNERLVTEDLADVLTRLEPIAKSLHCAD
                     ELRAVADIWADGASYQRQRRVAEEHDGDLRAVVDALVGELDI"
     gene            11561..12211
                     /locus_tag="VFI55_12355"
     CDS             11561..12211
                     /locus_tag="VFI55_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020731720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET7074861.1"
                     /translation="MADLEPIELAMFPLESALLPNQDLPLRIFEPRYSALVRRCIAGD
                     EPFGVVLISHGREVGGGDARCDVGMLCRITEYADLGAGRYALHCRTGDRIRVSEWLSD
                     DPYPRATVTLWPDEPGDPVTDAQLVDLEDRVVALFERVAQARGASLPDRDVLLGHGEG
                     DTNDAGQRLFALASRIPIGTADRYAVLSAPSAPDRLAALREAVDAVAEMVEFQLSE"
     gene            12401..12559
                     /locus_tag="VFI55_12360"
     CDS             12401..12559
                     /locus_tag="VFI55_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074862.1"
                     /translation="MSVVLIGPMSRCSNCGRVPPEGQRWPHATQGDRFCSLDCLYGLH
                     PELEEGGL"
     gene            complement(12664..13020)
                     /locus_tag="VFI55_12365"
     CDS             complement(12664..13020)
                     /locus_tag="VFI55_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074863.1"
                     /translation="MAEAALCMVWGAPVPGREKQALNAYNDAMQYWAGLQQEGKIERF
                     DVTVVAPSGGEVAGFLVTRGTAEQIDSLRRSKEYQRQVTRVQLVVSHLGVSDAYVDEG
                     IAQIMAQYQEVIAQEA"
     gene            complement(13116..13613)
                     /locus_tag="VFI55_12370"
     CDS             complement(13116..13613)
                     /locus_tag="VFI55_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074864.1"
                     /translation="MTPGQNWGATPAERTASLCCDAVFPRAVRADRAISIAAPPPIVF
                     SWLCQLRVAPYSYDILDNFGRRSPRERSPELVHLEIGQRFMTVFTLQSFAAGEQITLR
                     SKGVAVTYAVRPEHAGSRLHARVLIAGPSLIARALGLGDLVMMRRQLRTLKSLAEREA
                     AAACR"
     gene            complement(13610..14014)
                     /locus_tag="VFI55_12375"
     CDS             complement(13610..14014)
                     /locus_tag="VFI55_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014984284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074865.1"
                     /translation="MERLPYIDEHAITVEANRDDTWSALLRVMCRDPRDASSVPAGFV
                     LDEARAPERFALKGRHPFAVYRWVFELEPEPGDKAPHRTRLRALTWAAFPGVHGKIYR
                     ALVIGSGGHRIAVRWTLKRIAASADSRGAAVS"
     gene            14036..14668
                     /locus_tag="VFI55_12380"
     CDS             14036..14668
                     /locus_tag="VFI55_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014984283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET7074866.1"
                     /translation="MAPPRKHETDVILDATRALVLRDGPRAASVAAIAKASGAPAGTL
                     YHRFGNRDGVVTAAWLRALERFQSRALASGTNTAADTPADAALDIATAMAVATISFAR
                     DLPDDARMLLTIRPADLCDGQPDADFRDTLATMNAPLIQRVRELAARLYGSSGPRAVD
                     AVTRAVADLPYAVVRRHAHDEPMPSWLEADVAASVRALLQGPLGGLGERA"
     gene            complement(14642..16846)
                     /locus_tag="VFI55_12385"
     CDS             complement(14642..16846)
                     /locus_tag="VFI55_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7074867.1"
                     /translation="MPGAGDDAGSSGPAAIESGAARQLALTARLNTSAVDSRRGVIRL
                     HPNAVAALGIREWDAVSLTGSRTTAAVAGLAGPDIPVGTVLLDDVTLSNAGLREGTAV
                     LVGTVAVYGARSVTLSGSSMATQSLTPVTLRQALLGKVMTVGDAVSLLPRDLGPGTST
                     STATRALAAAVGISWTSELLTVTGVDPEGPVSVQPNSLVTWGSGVVASLREPVAATTP
                     EIPLEELKGAQPQAAKLSDWLKLSLDEPHLLKTLGASTNLGVLVSGPAGVGKVTLVRA
                     VCKGRRLIELDGPQVGALVADDRLKTVAAAVKRVRDKGGVLLITDVDALLPATAEPVA
                     ALILSELRTAVATEGVALIATSARPDQLDARLRAPDLCDRELGLPLPDAATRKALLAA
                     LLKRAPTGDLDLDEIASRTPGFVVADLAALVREAALRAASRASTDGQPPKLNQADLVG
                     ALTVIRPLSRSASEELTIGNVTLDDVGDMADAKQALTEAVLWPLQHPDTFARLGVDPP
                     RGVLLYGPPGCGKTFLVRALASTGQLSVHTVKGSELMDKWVGSSEKAVRELFRRARDS
                     APSLVFLDEVDALAPRRGQSFDSGVTDRVVAALLTELDGIDPLRDVVVLGATNRPDLI
                     DPALLRPGRLERLVFVEPPDAAARLEILRTAGKSIPLSADVDLENVAAGLNGYSAADC
                     VALLREAALTAMRRSIDAANVTAADLAAARKAVRPSLDPVQVESLRAFAKSA"
     gene            complement(16846..17709)
                     /gene="pssA"
                     /locus_tag="VFI55_12390"
     CDS             complement(16846..17709)
                     /gene="pssA"
                     /locus_tag="VFI55_12390"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HET7074868.1"
                     /translation="MIDKPRGRRAVNLQILPSAMTVLSICAGLTSIKFALEHQPKAAM
                     ALIAAAAILDGLDGRVARILDAQSRIGAEIDSLADAVNFGVTPAIVLYVTLLSNSPAG
                     WVVVLLYAVCVVLRLARYNALQDDGTQPAYAHEYFVGMPAPAGAVSMIGLIGLKLQFG
                     EGWWTSSVFLCIWVTGTSVLMVSKIPMRKMHAVSVPPSWAAPLLAVLAICAAAAVLAP
                     YVLIWVIIIAYLCHIPFAIRNRRWLAKHPEVWDDKPEQWRAARKATRRSQPNRRSVAR
                     LGLRKPSGRPR"
     gene            complement(17706..>18000)
                     /locus_tag="VFI55_12395"
     CDS             complement(17706..>18000)
                     /locus_tag="VFI55_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HET7074869.1"
                     /translation="GRFGSADLASASTENERTSLRIRTDTGSDVVAVQIAGLLARRIV
                     CDAGVGDKLSVGDTYGLIRFGSRLDTYLPPGTEPLVKVGQRAVAGETVLAELP"
BASE COUNT         2942 a         6028 c         6091 g         2939 t
ORIGIN      
        1 caattgcgag atccctcagc cggcgtcgca gccgactgaa taccacccgc agatcgcggg
       61 ccgccgccgc ggccgagtca ccggtgccgt ccacatcacc agtctagaat agacagttga
      121 gactgtccag tttcaactta agaagtcttc cgcgtcgggg ccgcgcccac ccagtagatg
      181 agaaaggcca tctccatcag ccacccgatg gcaagtaggg ggatcgccca gatgcggcgc
      241 gtgagcagcg cgctgccgca ctttgcggtc aagtcactgc tttccgcgac ctggctgaag
      301 tcggatttga agccgttgcc gcatttgatc ggaaagccgt acatgtcgtg ttggccgagg
      361 gaaatcggca gccacagcgc cgccaatccg atggccgcca gcgccaggcc cgccacgccg
      421 aggtagatct ggcgctttct cacggccgtg tccggtgggc cggcctcacg tcatgcagac
      481 gttggagaac agcaggaccg gccacgacac gatggacccg agaaaggaca ccgccaggtc
      541 gataccgtgc atctggtgga gatgctcggt gtgggtggtc gaccagaccg tgcccaccag
      601 cagatacgga gtgccgatga tcaatccgat ggcaatgagc tgaccgatgg tgagctggaa
      661 gtcgagcact tggcgaagtt tgtccaacat ctgctgtccc tccggcccgc caagtgggga
      721 tcattttaat ccagatccct gtcctcctgt ttggctcccc ggcgccccga gcagccgcag
      781 tgcggcatcg accgcgagcg cgggcacgtc gagggtcttc gagcgcagat cgtgtcgcgc
      841 gccggtgatc tcgacgagct cgatcggtgc ggcgactatc cgcgcggcgt cacgtatctc
      901 cgtgggcgtg ccgaaggggt cggccgttcc gtaggtgaac acggtaggaa cggtgatgtc
      961 gggcaggtgc tcggtgcgcg cgcgctccgg cttgccgggt gggtgcagcg gatacgagaa
     1021 cagggtgagc acgtctacct gtgcttgctt gtcggacacc accatcgacg tctgccgacc
     1081 gccgtaggaa tgtccgccga cgatcatcgg gccgtcggca aggcctcggc ataccgcgat
     1141 cgcctcgacg attccggcgc ggtctgcggc ggccgagccc gacggtggac cggtgggccg
     1201 gcgccggcgg tagggcaggt tataccgcac cgccagccag ccgcgcctag cccactcgtc
     1261 gcaaacctgt tgcagcagcg gcgattcccg gctacctccg gcgccatggg taagcaccac
     1321 gacaccgcgc gccggcgtgc ttgcgggttc atgcgcgaca cccgcgatgt ggtcgaggtt
     1381 cacgcgcccg ctgacgatgc ggtccggaac ggaccgaggt gggggcacca cccgcttgcg
     1441 ggggagaggc gggcaatcat gagagccgaa acaaggggga gaccggcccg tggccgtggc
     1501 ccaacggata ggcggcgcgc aggcattcgg tgacccagcg ctttccgaac ccgacggcgt
     1561 ccggcacgct gaacccgtgc gccaacgcgc acgccaccgc ggccgcgagc gtgtcaccgc
     1621 cgccgtggtc gttgccggtg gccaaacgcg gcgagtcgaa ctcgtggaag tcggcttcgc
     1681 caaacgcact tccataaagc aagtcgcagc tgcgatccga cgaccgcagg tgcccgccct
     1741 tgaccaaagc ccactgcggg cccagtgcgt gcaaggcctt ggccgccgcc cgctgcgacg
     1801 tcgcgtcgac cacatcgatg tctaccagta gccgaacctc gtcgaggttg ggtgtcacca
     1861 gcgtggccag cggaagtagt tgattgcgaa gggaatccag ggcctcgggt gccagcagcg
     1921 ggtccccgtg catggatgcg cacaccggat cgacgacgag tggaaccgtc agcccgagcc
     1981 ggcgccaagt ggcggccacc gcggcgatga tggccgagga cgccagcatg cccgtcttgg
     2041 cggactggat gccgatgtcg gtgaccacgg cttcgatctg accggcgacg acatcgtcgg
     2101 gcacctcgtg aaagctcttg acccccaacg tgtcctgcac cgtgaccgcg gtgaccgcga
     2161 cgcacgcgtg cacaccgagc agcgccatgc tgcgcatatc ggcctggatg ccggcgccgc
     2221 cccccgagtc cgatccggcg atggtcagca cgcgcagcgg cgtggtgccc ggcggcggta
     2281 cgggcagaac gagaggcagg taactcactg cgtgatcggc agatacaccc ggttgccgtg
     2341 ctcggcgaac tcgcgcgact tttcctccat gccgtcctgc atggccgctt cgatggcttc
     2401 ctcggtgtcg agcccgtggg cggccgcgta gtcgcggaca tcctgggtga tgcgcatcga
     2461 gcagaacttc ggtccgcaca tcgagcagaa gtgcgcggtc ttggccggct ccgcgggcaa
     2521 ggtctcgtcg tggaattctc gtgcggtgtc ggggtccaac gacaacgcga actggtcgtt
     2581 ccaccggaac tcgaagcgcg cggtgctcag cgcgtcgtca cgctcctgtg cacgcggatg
     2641 ccccttggcc aggtccgccg cgtgggcggc gatcttgtag gcgatcacac cgtccttgac
     2701 gtccttgcgg tccggcagcc ccaggtgctc cttgggcgtc acgtagcaca gcatcgcggt
     2761 gccggcctgc gcgatgatgg ccgcgccgat cgcagaggtg atgtggtcgt aggccggcgc
     2821 gatgtcggtg gccagcggac ccagcgtgta gaacggggcc tcctcgcaga gctcctcttc
     2881 cagccgcacg ttctccacga tcttgtgcat cgggacgtgc ccgggaccct cgatcatcac
     2941 ctgtgcgcca tgggctttgg cgatcttggt cagctcaccc agggtgcgca gctcggcgaa
     3001 ctgcgcggcg tcgttggcgt cggcgatcga gcctggccgc agtccgtcac cgagcgagaa
     3061 ggtgacgtcg tagcgcgcga agatgtcgca gagctcctcg aagttggtgt agaggaacga
     3121 ctcccggtga tgggccaggc accaggccgc catgatcgac ccgccgcggg acacgatgcc
     3181 ggtgacccgc ttggcggtca gcggcacgta ccgcagcagc acgccggcat gcacggtcat
     3241 gtagtccacg ccctgctcgc actgctcgag caccgtctcc cggtagatct cccaggtcag
     3301 ctcggtcggg tcgcccttga ccttctccaa cgcctggtag atcggcacgg tgccgaccgg
     3361 gacgggggag ttgcgcagga tccactcgcg ggtttcgtgg atgttcttgc cggtggacag
     3421 gtccatgatg gtgtccgcac cccagcgggt ggcccacacc atcttgtcga cctcttcggc
     3481 gatcgacgac gtcaccgccg agttgccgat gttcgcgttg accttgaccg cgaacgcctt
     3541 gccgatgatc atcggctcgc tctcggggtg gttgtggttg gccgggatca ccgcgcggcc
     3601 gcgggcgacc tcgtcgcgca ccaggtcggc gggcatgtcc tcgcgcgcgg cgatgtatgc
     3661 catctccgcg gtgatctcgc cggcgcgggc gcgttgcagc tgactgccgc gatcgcggac
     3721 cacgccgggc cgcgccggta acccggccgc cagatcgatc accgcttcgg tatccgtgta
     3781 cggccccgag gtgtcgtaga ggtcgaagtg gtcgccggta gacaggtgca cccgccggaa
     3841 cgggacgcac aagccgccgt ccaattcgcg gtaggccttg ctgctgcccg cgatgggtcc
     3901 ggtggtcacc gacggctcga tagttacagt catttcatct ccctacgccg gcattatccg
     3961 gtcaggttcg tacggtcgac ggccccaagc cgtcctctca gcgcactcgg cgtgcgctcc
     4021 cgcggctgtt ttcttgtctg gcgagatcgc ccgaacgcga cgttaccccc acccgagaca
     4081 tcgcgatacg cgggcagtcg gcacggcgaa aaatcagtgc gaattgccac gatgaggacg
     4141 tcctttctga tagagcggta gccggggttt ccgcccgaat gggaggatat aaacgtgcaa
     4201 cagcccacgc gaaagggctc ggtgacacca acggggaaca gcaagttgcc gcccggcgcg
     4261 accgaggcgg ccggcgagta tttgaacgtc ggcaccttcc gcttctggtt cgtcggtgaa
     4321 cgctgggaat ggtccgatga ggtcgccgcc atgcacggct acgaaccggg gaccgtcgcc
     4381 ccgacgacca aactgttgct gtcgcacaag catcccgacg accgtgcgca cgtcaaggac
     4441 ctgctcgact acgcgttgca gtcagaggag tcgttttcga gtcggcaccg gtttatcgac
     4501 accgccggca gagtgcacga cgccatcgtg gtagccgacc gcatgctgga tgaggccggc
     4561 gcggtgctgg gcaccgcggg ttattacatc gacctgaccg agaccttcga cgaggcccgg
     4621 tacgagactc gacaggaagt gctcgatgag gccctgccgg acttattcga gaaccgtgcg
     4681 gcgatcgagc aggccaaggg cgtgctgatg tacgtctacc gggtgagcgc cgaccaggcc
     4741 ttccgcgtcc tgcagtggcg gtcgcagcag accaatgtga aactgcgagc gctcgccaag
     4801 cagctgctcg ccgaaatctc caccctgatg cccccgacgg ccgccgtcca aagccagttc
     4861 gaccacgtgt tgttgaccgt gcacgaacgg atcccggccg agtcctgatc ggggcggtca
     4921 gacctcttcc atcgcctcgc cgagttcctc caggagacgg ttgtggtggt tgctcgccaa
     4981 gacgtgtccc gcggcgatga ccaacgcgac gggccagtcg atgagttcaa gtgccgccag
     5041 ggcggccaga cccccgtagt aggccagttg ttctgggcgc ggaatctcca tctgccccac
     5101 caacggcacg ttcaccacga aggtttcggc ttcgcggatc ttctccaccg cctcgcgctg
     5161 gggtgtcgct cggcgcgact tgttttccgc catcatttgc ctttcagtaa cgaagggttt
     5221 gccgcctcgc cgggtcgtgc ggtcaacgtc agattcatgt cgacgaccgg ctctgtggct
     5281 gatccccttg tttcgtcgat tgcgtcggtg ctgagggtgc cgttagttga actctatgcg
     5341 ctgctctggc gtgtcggggt ggtggagatt cggcaaacac gcggtgcaga actcaacgag
     5401 gcgcacccag cggtgcgccg ccgagcaact ggctgactgc tcagcgttcc gcgcggggtt
     5461 ttggcccaac ggttccgtct agttggtgtt acgcaacccg gtggcgcccg cccgacgtcc
     5521 gggtgccttc ttgacggcgg gcttactggt ggctttttcc gcaaccgcct tcttcgccgg
     5581 ctcggagggc actggcttga ggttcgcctt ggccggcgcg ggcggctggg gcttgcgctg
     5641 catcttgtgc agcaacaacg cgccgcctcc gacggccacc agcaacggcc attcgaccag
     5701 gccggcgacg ccgagggcac ctatcgccag cgcggccgcc ggtgtcgagt ggctgccgct
     5761 gccgattccg cgcttgattc cctcggcggc tcccgtgacg ccgccgacaa ggccattgac
     5821 ggcggcgcca cccacagcgc ccgcggccgc tgtggtcgcg tccgccgcac gacccacgct
     5881 tttgcccacg ttgcggactg ccccgccgac gaaactcatg atgactccct ggtacctaat
     5941 tgctctcgct gggaactgcg ttgggaactt ggatacccgt tgaacgcaaa gttaacagca
     6001 acaacgaaaa tgttctgacc atccatctag cgaaagcctg ccacgttaca aacgcgcgta
     6061 caacgagcaa cttctggatt ctgtgcggcg ccgaccctgc acacgaatca tccatgcgac
     6121 tcaaacgggg ctcacatcga tcagtaccgg cgcgtggtca ctgggtgcct tccccttgcg
     6181 ttcctcgcgg acgatctgcg cgtcggtcac acggccggcc aacgccggcg aggcgagtat
     6241 gaaatcgatg cgcatgccct gcctcttcgg aaaccgcagc tgggtgtaat cccagtaggt
     6301 gtaaacgccg gggccaggcg tgaaaggcct taccgcatca gtgaattgcg catcgacgat
     6361 agcgttgaac gcgaggcgct ccggctctga aacgtgggtg gcgccggcat agaactcggt
     6421 gctccacacg tcgtcgtccg ttggggcgat attccagtcg ccgaccaggg cgatcggtgc
     6481 cgcgggatcg tctcttagcc agccggacgc tgtatcacgc agtgcggcaa gccaattcag
     6541 tttgtaggtg tagtgcggat cgtccagcgc gcggccgttc ggcacgtaga ggctccacac
     6601 ccggatgccg ccgcaggtgg cgcccagtgc acgggcctcc gccgtggcgg ccacctccgg
     6661 cttgctactc cagctgggct ggccttcgaa gccgatctgc acatcgtcga gaccgacacg
     6721 ggaggcgatg gccacgccgt tccactgatt gacaccgacg tgtgcgacct cgtagccgag
     6781 ttcgaagaac ggcagggtgg ggaactggct gtcggaacat ttggtctcct gcatggccag
     6841 cacgtcgacc tcggcgcggg ccagccagtc gagcacgcgg ttcaaccggg tgcgaatcga
     6901 attcacgttc cacgtggcca gccgcagcag cgggtcgccg ccatcaatag ttgcgtcggg
     6961 catggctaga cgctatccat tggccgggcc cggacgtagc ggcattggtg gtgcagccgg
     7021 aacgccaggg agtcggcgag agtggtcggc gacccgtgct ggccgccgga ccggagatag
     7081 gctcgcgtcg cgccgcggcg cgtaccccag gccagcaacg cctcacacag cgcggccacc
     7141 gcacctggcg tcgactggtc atcgaccgca tgcagttgcg acaggcctac ccaacggatt
     7201 ccgtccggcg cgacggtcag cgccgcccgc gcgaccgctt ggtccgaatg ggtgccgaag
     7261 atcagctcgc cgtcgatgac ggcggtcagc acggcaacag gtacctctcg gtgtaggcgc
     7321 agccaggtgt caccgggccg tgccgacagg gcgatagacg ggtcgggctc tccgatggac
     7381 gcagcggaga cgtcgcgcac caggacgcgt tccgtgtgtt cgtccggcac gccggaaggc
     7441 agtagcaaca ggcgatcggg aatggccagt cgtggtgtca ggccgcgacg gtcgtaccag
     7501 gctacgattt cagggatcgc gcctgagtga gccgaaatat ccagtggcag agctgaattc
     7561 gcggcgaagt cggctccgtg tccggcccgc agcagccagc cgtccagcca ggtgtgttcg
     7621 gcgccgggcc aggccatggc ggccgcgtgt tcgagcgccc ggatttggga ggtgcggatg
     7681 ggtgtgtcgg tcagtacccg tagcgcgacg acgtcggtgg gcgcacactc ggcgacctcg
     7741 cccgttttcg tccgcacccg taccaccggg tcgaccgcca gcaggtgtcc gaccgcgtcg
     7801 gtcagcggcg gatccgatcc ggcggggcgc cggtagcgga ccgtcacccg cgttccgagg
     7861 tccggccacg agaccatcag tggccgaacg ggtcgggtcc ctcgcccggc gtccaggaca
     7921 gtccgggaac tccccagccg tgtgacttga cggcgcgctt cgcggcacgg gcgtgccggc
     7981 cgatcaggcg gtccaggtaa agaaatccat ccaggtgccc ggtttcgtgc tgcagcattc
     8041 gggcgaacag gccggtgccc tcgatcgcga cccgacgacc gtcggcgtcg agcccggtca
     8101 ctcgcgccca cttcgcccgg ccggtgggga aggactcgcc gggaaccgac aagcacccct
     8161 cgtcgtcgtt gccgggatcg ggcatggtct cgggtatttc ggaggtttcc aacaccgggt
     8221 tgatcaccac gccgcgacgg cgatcagtca gtccgcggtc ctcggagcag tcgtagacga
     8281 acaaccgcaa accgaccccg atctggttgg cggccaggcc gaccccgtgt gcggcgtcca
     8341 tggtgtcgta catcgttgag atcaacgcgg ccagatccgc cgggagcgaa ccgtctgcgg
     8401 cgacggccac cggcgtggtc ggggtgtgca ataccggatc tccgacgatg cgtatgggta
     8461 cgactgccat ggtcggctag cttaagccgg gtgacgaggc acggcgcgga gcggcgtgct
     8521 gaggaggcgg gccatcgggt taagccgggt gacgaggcac ggcgcggagc ggcgtgctgt
     8581 gggggcacct cccgcttgcg ggggagaggc gggccatcgg gttaagccgg gtgacgatgc
     8641 gcggcgcgga gcggcgtgct gtggaggggg gccatcgggt tagcccgctg ggggcaatcg
     8701 cagcgaatcg gccgactcgc gggtctgcat tacggcttga gggttcgcgg catgtttcaa
     8761 tattcgccga aacatgcccc gatgtaagtc aagtcattcc aacacattga gcaccgccgg
     8821 aagggtccag gggaagcgat atggacagcg ccatggcgcg ggcaaatcga tcgggggacg
     8881 actctgagat cgccgatgga ctcacccgcc gtgaacacga catcctggcg ttcgaacgtc
     8941 agtggtggaa attcgccggc atcaaggaag atgcgatcaa agagctgttc tcgatgtcgg
     9001 cgacgcgtta ctaccaggta ctcaacgctt tggtcgatcg acccgaagcg ctcgccgcgg
     9061 acccgatgct ggtgaagcgg ctgcgtcggc tgcgtgccag ccggcagaag gcgcgggcgg
     9121 cccggcgcct cggctttgag gtgacctgac aagcccgtgc ctttgcgggc tctggcgttt
     9181 ttctggctac agtgggctcg atgaaagagc gcgttcccga ctcgaccggg cttcccctgc
     9241 gggccatggt gatggtgctg ttgttcttgg gcgtcatttt cctgttgctc gggtggcagg
     9301 cgctgagctc gtcgtcgaac tccgatgacg actcggcgtc gccggtctcc agcgtcacca
     9361 gcaccaccgc tccggtgtcc acttcgacga gcgctaagcc gccggcgaac gacgccgagg
     9421 tgcgcattta caacatctcc tcgaaagagg gcgtggccgc acacaccagg gacgacctcg
     9481 cagccgccgg tttcaaggtg accgatgtcg gcaacctctc gttgcccgac atcacggcca
     9541 ccacggtgta ctacaccgat gccgacggcg agcacgccac ggcggacgct gtgggccaaa
     9601 agctcggggc gcccgtcgaa ccccgaattc ccgcactcag caaccagccg cccggcgtga
     9661 tcgttctggt ggcgggttga gtgccgggcc gcgactgtaa cgtcagcgcg aaaaagccgg
     9721 cacgaattcg cagtagcgtt acgctcgcgc cggacgtcgt cgggctaggc tctcgctatg
     9781 ctcaaactct ccccgctgtt gctgggctcc tgcgtggtgc tggcaagcgc gtgttcgtcg
     9841 ccacaacacg catcgacgac cccgggaacc accccggcga tctggacggg atcaagcgcg
     9901 ccgtcgggcc cctcgggtac cgagggtgga cagcagccgg tggcgcaggc cgtgatcacc
     9961 cacctgaggg cgcctgacgg cacccagatc gcaaccgcca agttcgattt cggcaacggc
    10021 tacgccaccg tcacgatcgc gacgaccggg gtcggccagc tcacgcccgg cgtccacgcg
    10081 gtacacatcc acaaggtggg caagtgcgag cccaactcgg tggccccaac cggcggcgcg
    10141 cccggcgact tcctctctgc cggcgaacat ttccaggcgc ccgggcatgc ggccgagccc
    10201 gccagtggtg acctgacgcc actggaagtt cgcaaggacg gttccgggat gatggtgacc
    10261 accaccgacg ccttcgccat ggacgatctg ctcagcggtg agaaaaccgc gatcatcatt
    10321 cacgccggtg ccgacaacgc ccccggcggc atgccgggtc ccgacgggat ggcgatgggc
    10381 actggtgacg ccagcaagcg ggtggcatgc ggtgtcgtcg gtgccggcta gcggggccaa
    10441 aacccgcatc gatttcgccc cgtccccgga gggaaccatc ggtgtggagt gggagttcgc
    10501 gctcgtcgac gcgcagaccc gcgacctgag caacgaagcc accgcggtca tcgccgaaat
    10561 cggcgaaaac ccgcgcgtgc acaaggaatt gctgcgcaac accgtcgaag tggtcagcgg
    10621 catctgtgag tgcaccgcag aggcgatgga agacctgcgc cagacgttgc gtccggcgcg
    10681 tcaggtggtg cgggaccgcg gcatggagct gttctgcgcg ggcacccacc cgttcgcccg
    10741 gtggtccgcc cagaaactca ccgacgcccc gcgctacgcc gagctgatca aacgcaccca
    10801 gtggtggggc cgtcagatgc tgatctgggg ggtgcacgtg cacgtcggga tctcctcggc
    10861 gaacaaggtg atgccgatca tgacttcgct gctgaactac tacccgcatc tgttggcgtt
    10921 gtctgcgtcg tcaccgtggt ggggcggcga agacaccggg tatgccagca accgcgccat
    10981 gatgtttcag cagctgccca ccgcgggcct gcccttccac tttcagacct gggcggaatt
    11041 cgaaggcttc gtctacgacc agaagaagac cggaatcatc gaccacatgg acgaaatccg
    11101 ttgggatata aggccttcgc cgcatcttgg caccctcgag gtgcgggtct gcgacggggt
    11161 gtccaacttg cgtgagcttg gcgcgctggt tgcgctgacg cactgcctgg tcgtcgacct
    11221 ggatcgccgg ctggacgccg gtgagacgct gcccaccatg ccgccgtggc atgtgcagga
    11281 gaacaaatgg cgcgccgccc gctatggcct ggacgcggtg atcatcttgg acgccgacag
    11341 caacgagcgg ctggtcaccg aggacctggc cgatgtgctg acccggctgg agccgatcgc
    11401 gaagtcgttg cattgcgccg acgagctgcg cgcggtggcc gacatctggg ccgacggtgc
    11461 ctcataccag cgccagcgac gggtcgccga ggaacatgac ggcgacctgc gtgccgtcgt
    11521 cgatgcgctg gttggcgagc tcgacatcta ggtttaggtc atggcggatc tcgagccgat
    11581 tgagctggcg atgttcccgc tcgagtcggc gttgctgccc aatcaggatc tccccctccg
    11641 cattttcgag cctcgctaca gcgcgctggt gcggcgctgc atcgccggtg acgaaccgtt
    11701 cggcgtcgtg ctgatctcgc acggccgcga ggtcggtggc ggggacgccc gttgtgatgt
    11761 cgggatgttg tgcaggatca ccgaatacgc cgatctcggc gcgggccgat acgcactgca
    11821 ctgccgcacg ggggaccgga ttcgggtgtc cgaatggctg tccgatgatc cctacccgcg
    11881 cgcgaccgtg acgctgtggc ccgacgagcc cggggacccg gtgaccgacg cccaactcgt
    11941 cgatctcgaa gaccgggtgg tggccctgtt cgagcgggtc gcgcaagctc gtggcgcttc
    12001 gctgccggac cgcgacgtat tactgggtca cggcgaaggc gacacgaacg atgcggggca
    12061 acggctgttc gcgcttgcgt cgcgcatccc gatcggaacg gccgaccggt atgcggtcct
    12121 gtcggcgccc tcggcccccg atcgattggc cgcgctgcgc gaggccgtgg acgcggtcgc
    12181 ggagatggtg gagttccagc tgtccgaata gggcctaccg ggcgtcgcaa cgtcatgaca
    12241 tgcggcgcgg cggcgcccag gttaaagtcc tgccatcttg cctctgccgc cgaagccaga
    12301 gttcccgcaa ggcatgaata ggaccggcat tgttcgggcg aacagagccg aggcgcagtc
    12361 gcagagactg cggccgatcc cacgaatgac cggagcagcg atgagtgtcg tattgatcgg
    12421 gccgatgagc cggtgtagca actgcggccg cgttccgccg gagggccagc gctggccgca
    12481 tgccacccaa ggcgaccggt tctgcagcct ggactgtctg tacgggctgc atcccgagtt
    12541 ggaggaaggc ggcctgtaag ccggcgccgg ccccggtgac cgcctgacca gacgcgtcta
    12601 accatcgccg ctaccgagtc tgcaacggcg tcttgcgacc tcgaggggcc ggggcctggt
    12661 agctcaggcc tcctgtgcga tcacttcctg gtactgggcc ataatctggg cgattccctc
    12721 gtcgacgtaa gcgtccgaga cgccgaggtg ggacacgacg agctgtaccc gggtgacttg
    12781 tcgctgatac tccttgctac gccgtagcga atcgatctgc tccgcggtgc cgcgcgtgac
    12841 aaggaacccc gccacttctc caccggacgg cgcgacaacg gtcacgtcga agcgctcgat
    12901 ttttccttct tgttgaagac ccgcccagta ctgcatcgcg tcgttgtagg cattgagggc
    12961 ttgcttctcc cgcccgggta cgggcgctcc ccaaaccatg cacagagcag cttcagccat
    13021 ttgacttcct ctctcttcag cccctcgagc ccccaatgcg gattgtctcg cggatcgccg
    13081 ccaaccacac gagtacgccg gtacctgatt ttcggctacc ggcacgccgc cgcggcttcg
    13141 cgctcggcca gtgacttcag cgtgcgtagt tgcctacgca tcattacgag atcgcccagc
    13201 cccagcgccc gagcgatcaa cgacggaccc gcgatcagca cgcgcgcatg aagccgcgag
    13261 cccgcgtgct cgggccggac cgcgtacgtg acggcaacgc ccttggaccg caacgtgatc
    13321 tgctcgccgg ccgcaaacga ctgcagcgta aagactgtca tgaatcgctg cccgatttcg
    13381 aggtggacca gttcgggcga acgctcgcgc ggacttcggc ggccgaagtt gtcgaggatg
    13441 tcgtagctgt agggcgccac gcgtagctgg cacaaccagg agaacacgat cggcggcggc
    13501 gcggcgatgc tgatcgcgcg gtcggcccgg acggcgcgcg gaaagaccgc gtcgcagcac
    13561 agggatgccg ttcgttccgc cggcgttgct ccccagttct gaccgggggt caagagacag
    13621 ccgctccgcg cgaatctgcc gatgccgcga tgcgtttcag cgtccaacgc acggcgattc
    13681 ggtggccgcc ggagccgatg acgagcgcgc gatagatctt gccgtggacg cccgggaagg
    13741 ccgcccacgt cagcgcgcgc agccgggtgc ggtgcggagc cttgtcgccg ggctcgggtt
    13801 ccagctcgaa cacccaccga tagaccgcga acgggtggcg gcctttcagt gcgaaccgct
    13861 cgggtgcccg agcttcgtcc aggacgaaac cggccggcac gctcgacgcg tcgcgcgggt
    13921 cgcggcacat cacgcgcagc agcgccgacc acgtgtcgtc gcgattcgcc tccaccgtta
    13981 tggcatgctc atcgatatag ggcaatcgtt ccatatagaa ggactgtact agatcgtggc
    14041 ccctccccgg aagcacgaaa ccgatgtgat cctggacgcg acgcgtgcgc tggtgctcag
    14101 ggatggaccg cgggcggcga gcgtcgcggc gatcgccaag gccagtggcg ccccggcggg
    14161 cacgctgtat caccggttcg gcaatcgcga cggcgtggtg accgcggcgt ggctgcgtgc
    14221 cctcgagcgt ttccagtcgc gagcgctggc ctctggaacc aatacagccg ccgatacgcc
    14281 ggcggatgcc gccttggaca tcgcgacagc gatggcggtc gcaacgatca gcttcgcgcg
    14341 cgacctaccc gacgacgcgc ggatgctgct gacgatccga ccggccgatc tgtgcgacgg
    14401 ccaacccgac gcggatttcc gggacaccct ggccaccatg aacgcaccgc tgattcagcg
    14461 ggtccgcgag ttggccgcgc gcctctacgg cagcagcggg ccgcgcgcgg tcgatgcggt
    14521 gaccagggcg gtggccgacc tgccttacgc cgtggtgcgc cgccacgccc acgacgagcc
    14581 gatgccgtcc tggctggaag ccgatgtcgc ggcgtcggtg cgcgcgctgc tgcagggccc
    14641 cttaggcgga cttggcgaac gcgcgtaacg actccacttg aacgggatcc agcgagggcc
    14701 gcacggcctt gcgtgccgcg gcgaggtcgg cggccgtgac gttggcggca tcgatcgatc
    14761 gccgcatcgc ggtgagcgcg gcctcccgca gcagggccac gcagtcggcg gcgctgtaac
    14821 cgttgagccc ggcggccacg ttttccaggt cgacatcggc gctcagcgga atcgacttgc
    14881 ctgcggtgcg caggatttcg agacgggccg cggcgtcggg cggttcgacg aacaccagcc
    14941 gttccagccg tcccgggcgc agcagcgcgg ggtcgatcag atcgggtcgg ttggtggcgc
    15001 ccagcaccac cacgtcgcgc agcgggtcga tcccgtcgag ctcggtcagc agcgcggcga
    15061 ccacgcgatc ggtcacgccg gagtcgaagc tctggccacg ccggggcgcc agcgcgtcca
    15121 cctcgtcgag gaacaccagc gacggcgctg agtcccgggc gcgtctgaac agctcgcgca
    15181 ccgccttctc cgagctaccc acccacttgt ccatcagctc ggagcctttg acggtatgca
    15241 cgctcagctg gccggtgctg gccagcgcgc ggaccaggaa ggtcttgccg cacccgggcg
    15301 gcccgtagag cagcaccccg cgcggcgggt cgacacccag ccgggcgaag gtgtcgggat
    15361 gctgcagcgg ccacagcact gcttcggtga gggcctgctt ggcatcggcc atgtcgccga
    15421 cgtcgtccag ggtgacgttc ccgatggtca gctcttcgct ggccgaccgg gacagcggcc
    15481 ggatgacggt cagcgcaccg acgagatcgg cctggttcag cttgggtggt tgcccgtcgg
    15541 tgctggcccg agacgccgcc cgcagcgcgg cttcgcgcac cagtgcggcc aggtcggcga
    15601 cgacgaaacc cggtgtgcgg gaggcgatct catcgaggtc gaggtcgccg gtgggcgccc
    15661 gtttgagcag cgccgccaac agcgccttgc gggtggcggc gtcgggcagt ggcaggccca
    15721 gctcacgatc gcacaggtcg ggggcacgca gccgggcgtc cagctggtcg ggccgcgcgg
    15781 acgtggcgat caatgcgaca ccctcggtgg ccaccgcggt gcgcagctcg ctcaggatca
    15841 gggcggccac cggctccgcg gtggcgggga gcagggcatc gacgtcggtg atcagcagca
    15901 cgccgccctt gtcgcgaacc cgcttgaccg ccgcagccac cgttttcagc cggtcgtcgg
    15961 cgaccagtgc gcccacctgc gggccgtcca gttcgatcaa cctgcggcct ttgcacaccg
    16021 cacgcaccag cgtcaccttg ccgaccccgg ccggacccga caccagcacg ccgaggttcg
    16081 tgctcgcgcc caacgtcttg agcaggtgcg gttcgtcgag cgacagcttg agccaatcgc
    16141 tgagcttggc ggcctggggc tgcgcgccct tcagctcctc gagggggatc tccggggtcg
    16201 tcgcggcgac cggttctcgc aaggacgcca cgacgccgct gccccaggtg accaacgagt
    16261 tcggctgcac gctgacgggt ccctcgggat cgacgcccgt gacggtcagc agctccgagg
    16321 tccagctgat cccgacggcg gccgccagtg cgcgggtcgc ggtcgacgtc gatgtgccgg
    16381 ggcccaggtc acgcggcagc agagacacgg cgtcgccgac ggtcatcacc ttgccgagca
    16441 aggcctgtcg cagcgtcacc ggagtcagcg actgggtggc catcgaggag ccactgagcg
    16501 tcaccgatcg tgcgccgtag acggcgaccg tgccgacgag caccgcggtg ccctcccgca
    16561 gcccggcgtt ggacagcgtc acgtcgtcca gcagcacggt gccgacgggg atgtccgggc
    16621 cggccagtcc cgccaccgcc gccgtggtcc gcgacccggt cagcgacacc gcgtcccact
    16681 cgcggatgcc gagggcggca acggcattcg ggtgaagtcg gatgacgccg cggcgggagt
    16741 caacggcgga ggtgttcagc cgcgcggtga gggcgagctg acgggccgcg ccggattcga
    16801 tggccgccgg gccggagcta cccgcatcgt cgccggcccc gggcatcacc gcgggcgccc
    16861 gctgggcttg cgcagcccga gccgcgccac cgaccggcgg ttgggctggg accggcgggt
    16921 cgctttgcgt gcggcgcgcc attgctcggg tttgtcgtcc cacacctcgg ggtgcttggc
    16981 aagccagcgc cggttgcgga tcgcgaacgg gatgtggcac aggtaggcga tgatgatcac
    17041 ccagatcaac acatagggcg ccagcaccgc ggccgccgcg cagatcgcca gcaccgccag
    17101 cagcggcgca gcccagctcg gcggcaccga cacggcgtgc atcttcctca tcgggatctt
    17161 gctgaccatc agcaccgacg tgcccgtcac ccagatgcac aggaacacac tcgacgtcca
    17221 ccagccctcg ccgaactgca gtttcagccc gatcagaccg atcatcgata ccgcgcccgc
    17281 cggcgcgggc atcccgacga agtattcgtg cgcgtaggcg ggctgggtcc cgtcgtcctg
    17341 cagcgcgttg tatctggcca gccgcaacac cacgcacacc gcgtacagca gcaccaccac
    17401 ccaaccggcc ggcgagttcg acagcagcgt cacgtacagc acgatcgcgg gcgtcacccc
    17461 gaagttcacc gcgtcggcca gcgaatcgat ttccgcgccg atccgcgact gggcgtccag
    17521 gatgcgggcc acccggccat ccaggccgtc caggatcgcg gcggcggcga tcagcgccat
    17581 cgcggccttg ggctgatgct ccagcgcgaa tttgatcgag gtcaggcccg cgcagatgga
    17641 cagcaccgtc atcgcgctgg gcaggatctg caggttgacc gctcgcctgc cgcggggctt
    17701 gtcgatcatg gcagctcggc cagcactgtc tcgcctgcca ccgcacgctg acccaccttc
    17761 accagcggtt ccgtgcccgg tgggaggtaa gtgtccagcc gtgacccgaa ccggatcagc
    17821 ccgtaggtgt ctccgaccga cagcttgtct ccgacgcctg cgtcgcagac gatgcggcgc
    17881 gccagcagcc ccgcgatctg cacggccacc acgtcggaac ccgtgtcggt ccggatccgc
    17941 aggctggtgc gctcattctc ggtgctcgcc gaagccaggt cggccgaccc gaagcggccc
//
LOCUS       DASSGQ010000282        10293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1357272,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000282 DASSGQ010000000
VERSION     DASSGQ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10293
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357272"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="VFI55_12400"
     CDS             <1..1476
                     /locus_tag="VFI55_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7074870.1"
                     /translation="AGATPQRTVLVDGDIRLDCVTLHEQAAQMANALMARMPGGSVVS
                     FMLPNWHEAAVIYLAATLAGMVVNPILPSLRDHDLRFILEDADSRMVFVPSSFARHDY
                     TAMLARVTAQLESPPDVVVVRGEPGAGQAPFAPLLVERPAARALPTLDPDAVRMILYT
                     SGTAGRPKGVLHTHNSIHALIRQIREHWFVEPGDRFLVASPIAHIGGSIYAFECPLLL
                     GTTAVLMDRWNGDDAVELLATRRCTHMAGATPFLEQTLAAAQRAGTRLPGLKLFICGG
                     ASVSPSLIRRAAAHFESSVVTRVYGSTEVPVTTIGSPRHADHAADTDGHIGVADVKLV
                     AGEIRVRGPQMLVGYQHREDEVEAFDDEGYFRTGDLAHWVDDEYLVVTGRAKDIIVRN
                     GVNISPKEVEDILTGHPGIAEIAIVGLPDDRTGERACAVVVPAVTPGPDVTSLRDFLA
                     DSGVARFKAPEQVVIWDVLPKNDAGKILKHQIKAALMEADQ"
     gene            1487..2269
                     /locus_tag="VFI55_12405"
     CDS             1487..2269
                     /locus_tag="VFI55_12405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7074871.1"
                     /translation="MPVMPVAIVTGASGGIGSGCAAKLAEMGMAVLATGRNEGKLAQL
                     AELIGDHERVATLAVDLRDDDAPKRIADLAVARWGHIDFLVNIAGIGSPKPVHETDDE
                     SLDHFLDLMLRAPFRLIREVLPYLRPGSAIINVTSTFAVVGGLRGGAYSAAKGGLTAL
                     TTHVACQYGAQGIRCNAVAPGVIQTPMTEHRLQDERFRRINTEMTPHQRLGTVDDVAN
                     TVAFLCSPAGAFINGQTIVVDGGWSSTKYLSETALNSEWKPR"
     gene            complement(2303..2773)
                     /locus_tag="VFI55_12410"
     CDS             complement(2303..2773)
                     /locus_tag="VFI55_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7074872.1"
                     /translation="MSWWLARAQATLSEDVPAPPGDVRDFYVDLDNIKLVHPLIVSVQ
                     ATSRTETPEGYLQSYRVVDRIPLGPLTIKTAYRARLHVRADGDVATEADQSPGVHLRG
                     TVSFEPIREPVVGTRMTERIVISAPRPLAALTTREAVKAHIAMLAGIRRHFESA"
     gene            2836..3231
                     /locus_tag="VFI55_12415"
     CDS             2836..3231
                     /locus_tag="VFI55_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7074873.1"
                     /translation="MANAAEIGAGVVERYLECLAAHDWDGLATTIADEGLIREGPFCD
                     VVEGKQHYVAYLRKVLTNLEGHRLEVQRVSHVDSRVSYVELTEAFEIDGVAATWPECI
                     LFELGDDGLICHVSVFFKQRGADRAADGA"
     gene            3329..3562
                     /locus_tag="VFI55_12420"
     CDS             3329..3562
                     /locus_tag="VFI55_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074874.1"
                     /translation="MESHGTPELVPVEKLHSGDPVTDVNGGGQRYIVLESKTLGDSCV
                     VLELESRVDHQLQVIEKSFPTGYHFGRAHHRIL"
     gene            3921..8747
                     /locus_tag="VFI55_12425"
     CDS             3921..8747
                     /locus_tag="VFI55_12425"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET7074875.1"
                     /translation="MKIPGVASVVVGVTGGAAQVVRAGVSTAAGAAGAVQTLAGPVFE
                     LAGPVVQSMSQSTRRAIGIGVSPDGSADRIAPPVRWQSGRRVHLDLDPLLPFPNWNEH
                     AAVVEEPVRRIPGVAAAHVEGALGRLVIELETEIDSAMVLDDVRDTVCCVAADLASAG
                     PKSAPRTAPFADPGNPLAILVPLTAAAMDAAAIGAAVTGWVVRLPVAPRSARAAVALL
                     NHQPRVVAVLESRLGRVGTDIALSASTALANGLTQAVGTPLLDLAQRGLQISEAAAHR
                     ERWREREPELASPKRPQAPVVPVISSAGANSQAPRHNWAAAAAGDVSHVMVGGAIDAA
                     IDTAKGSMAGPVEEYADQAANASLIAAAGALLAGGGTEDAAGAILAGVPRAAHVGRQA
                     FASVLGRGLANAGQLILDPGALRRLDRVKVIVIDGAALRGDARAVLEARGDIPGWDED
                     RVYEVADALLHGEEAPDPDPDELPATGARLRWVRAQGSSAAPAQGLEHADLVVDGERA
                     GSVEVGWEVDPFAIPLLQTANRTGARVVLRHVAGTEDLTPSAAVTHPPGTPLLKLVRD
                     LRTDRGPVLLITSLHPDFASTDTLAALAIADVGVALDDPHAATPWTADIITGTDLAAA
                     VRILSALPVARDATESSVRLAKGGSTLSGLLLVSGEPRSANPIALQRWLNPVNAAGTA
                     ALISGAFSASRVLRLPDPTPQPLTAWHALDPEIVYARLTSRTRPLAVEAGVTPWRQFL
                     DDLSYNPAVAPLRGPATQVVRLLGATRAELADPLTPILAVGAAASAIVGSSIDALLVA
                     GVMTANALAGGVQRLRAEAAIAELFADQDQHARRVVLPTVATARRRLDAARSTERTVT
                     VSAKSLRPGDIIDLTAPEVVPADARLLVAEDLEVDESFLTGESLPVDKQVEPVAVADA
                     ERASMLFEGSTIVAGHARAIVVATGAGTAAQRAISAISDVESAAGVQARLRELTSKVL
                     PLTLAGGAAVTGLALAHRTSLRQAVADGVAIAVAAVPEGLPLVATLAQLAAAQRLSRR
                     GALVRAPRAIEALGRVRTVCFDKTGTLTENHLRVVSAVPHGISAQGSFPDADDPRATA
                     VVRAAARACTQPHNGQGHAHATDEAILTAANSLDGQRNSEWTVLAEVPFESSRGYSAS
                     IGAASDDTAGPMLMVKGAPEEILPRCRFADQEADLDDAESLILRLAEQGLRVLAVAQR
                     RWDRGTTEEDTDADAVDAAAHDLELVGYVGLADTARPSARPLIEGLLDSGHRVVLITG
                     DHPVTARAIARQLGLPADAREATGAELASLDEDARAKLAADVQVFARVSPEQKVQIVA
                     ALQQGGQVTAMVGDGANDAAAIRMADVGIGVSGRGSSAARGAADIVLTDNDLGVLLDA
                     LVEGRGMWGGVRDAVGILVGGNVGEVLFTIIGTALGTGRAPVGTRQLLLVNLLTDMFP
                     ALAVAVTPQYPQPEETEDGVDRDAEEMQRAFQLAALAEPAPSLDAPLMRQIVTRGAVT
                     AAGATAAWAIGRWTPGTERRTSTMGLTALVTTQLAQTLLTRRHSPLVLATALGSAGVL
                     VGIVQTPVISQFFGCTPLGPIAWSGVISATAGATAVSVLAPNWLANRVAALEPKQD"
     gene            complement(8865..>10293)
                     /locus_tag="VFI55_12430"
     CDS             complement(8865..>10293)
                     /locus_tag="VFI55_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7074876.1"
                     /translation="AARWPNRAAIIDDDGVLTYRELRSGIESLARELSGHGVGPGHAV
                     GVMCRNGRGFVRAFFAAVLVGADVVPINTEFRIDALAAALSAHRITTTVADDEFAGRL
                     READASAVVDPASADAHDGDSRPRVSAPGRIVLLTSGTTGTPKGVPRAPRIASALGVW
                     VTILDRTRLRTGSRISVAMPMFHGLGLGTLMLTLALGGTVLTRRHFDAETALAQASLH
                     RADAFTVVPIVLARILDLPQRVRARNPLPYLRVVLSSGDRLDPSLGQRFMDAYGDILF
                     NGYGSTEVGIGALATPADLRDAPETVGRPVAGGRVRIFDKKDRPVGPRVTGRIFVGGE
                     MTSEGYTGGSIVGEAKATIDNMTGTGDMGYFDNTGRLFIVGREDDMIISGGENVYPRA
                     VENALAEHPDIAENAVIGVLDERFGQRLAAFVVLQPGSDIDASALREYLQGKVSRFEQ
                     PRDIHIVAGIPRNPAGKVVRKELLT"
BASE COUNT         1610 a         3469 c         3557 g         1657 t
ORIGIN      
        1 gctggagcca ctccgcagcg aacagtgttg gtggacggcg atattcggct cgactgcgta
       61 acgctgcacg agcaggcggc acagatggcg aacgcactga tggcgcgcat gcccggcggc
      121 agcgtggtgt cgttcatgct gccgaactgg cacgaggccg ccgtcatcta tctggccgcg
      181 acactggccg gaatggtggt gaacccgatc ctgccgtcgc tgcgcgacca cgacctgcgt
      241 ttcatcctcg aagacgccga tagccgcatg gtgtttgtcc catcaagttt cgcgcgtcac
      301 gactacaccg cgatgttggc cagggtgaca gcccagctgg aatcgccacc cgacgtcgtg
      361 gtggtccgcg gggagcccgg cgccggccag gctccttttg cgccactgtt ggtcgagcgc
      421 ccggccgcca gagcgctgcc caccctcgac cccgacgccg tgcggatgat cctctacacc
      481 tctgggaccg ccggccgccc gaaaggtgtt ttgcacacgc ataattcgat ccacgctctg
      541 atccggcaga tccgtgagca ctggttcgtc gagccgggtg accgatttct ggtcgcgtct
      601 ccgatcgccc atatcggcgg ctccatctac gcgttcgagt gtccgttgct gctgggcacc
      661 accgccgtgc tgatggatcg atggaacggc gacgacgcgg tcgagctgtt ggcgacgcgg
      721 cggtgcaccc acatggccgg ggcgacacca tttctggagc agacgctcgc cgccgcgcag
      781 cgcgccggca ctcgcctgcc cggcctgaag ctgttcatct gcgggggggc atccgtgtcg
      841 ccgtcgttga tccgcagggc cgcagcgcat ttcgagtcat cggtggtgac ccgggtatac
      901 ggctcgacgg aagtgccggt gacgacgatc ggatccccgc ggcatgccga ccacgccgcc
      961 gacaccgacg gacatatcgg cgtggccgac gtcaaattgg tcgccggaga aatccgggtg
     1021 cgcggaccgc agatgctggt cggctatcaa catcgcgaag acgaggtcga agcttttgac
     1081 gacgagggtt atttccgcac cggggatctc gcacactggg tcgacgacga atacctcgtc
     1141 gtgaccgggc gggcaaaaga catcatcgtc cgcaacggcg taaacatctc acccaaggag
     1201 gtggaggaca tcctgaccgg ccaccccggc atcgccgaga tcgccatcgt cgggctgccc
     1261 gatgaccgca cgggagagcg ggcgtgcgcc gtggtggtgc ccgccgtcac gcccggcccg
     1321 gacgtcacca gcctgcgcga tttcctggcg gactcgggcg tcgcgcgatt caaagcgcct
     1381 gagcaagtgg ttatttggga tgtcctacca aagaacgacg cgggaaaaat actcaagcac
     1441 cagatcaagg cggccctgat ggaagcagac cagtgatatg ccggtaatgc cggtaatgcc
     1501 ggtggcgatc gtgaccgggg caagcggcgg aatcggatcc ggttgcgcgg caaagctcgc
     1561 cgagatgggc atggcggtgc tggcgaccgg acgcaacgag ggcaagctcg cccaattggc
     1621 cgagctcatc ggcgatcacg aacgggtggc cacgctcgcc gtcgacctga gggacgacga
     1681 tgcccccaag cgaatcgcgg atctggcggt tgcccgatgg ggccacatcg acttcctcgt
     1741 caacatcgcc ggcatcggca gtcccaagcc ggtgcacgaa accgacgacg aatcattgga
     1801 ccatttcctt gatttgatgt tgcgcgcgcc gtttcgcctg attcgtgagg tgctgccgta
     1861 cctgcggccc gggtcggcga tcatcaacgt cacgtcgacc ttcgctgtcg tcggcggcct
     1921 tcgtggcggc gcgtattcgg ccgccaaggg cgggctcacc gcgctgacca cgcacgtcgc
     1981 ctgccaatac ggagcccagg gaatccgctg taatgccgtg gctcccgggg tcattcagac
     2041 gccgatgacc gagcaccggc tgcaggatga gcgcttccgc aggatcaaca ccgagatgac
     2101 accgcatcag cgtttgggga cggtcgacga cgtcgcgaac actgtggcgt tcttgtgctc
     2161 tcctgcgggt gcgttcatca acggacagac gatcgtcgtc gacggcggct ggagctcgac
     2221 gaagtacctc tcggagacgg cgctgaattc ggagtggaag ccgcgctgag accgcgctga
     2281 gacggcgttg aaaccgcacc gctcacgcgg attcgaagtg gcgccggatg cctgccagca
     2341 tcgcgatgtg cgccttgacc gcctcgcgcg tcgtcagcgc ggccaacggc cggggcgcgc
     2401 tgatcacaat tcgctcggtc atccgggtgc cgactactgg ctcccggatc ggctcgaagc
     2461 tcaccgttcc gcgcaggtgc accccggggg actgatcggc ctcggtcgcg acgtcaccgt
     2521 cggcccggac gtgcagccgc gcgcggtagg cggtctttat ggtcaagggc cccaacggaa
     2581 tccgatctac cactcgatag ctctgcagat agccctccgg tgtttcggtg cgcgacgtag
     2641 cttgcacgga cacgatcagc ggatgcacca gtttgatgtt gtccagatcc acgtagaaat
     2701 ctcggacgtc gcccggcggc gcgggcacgt cttcggacag cgtcgcctgg gcacgcgcga
     2761 gccaccagct cataggagcg aacctactcg ggggcttacc gtggacctag ccatcggcga
     2821 gtgagtggag acgacatggc gaacgcggct gaaatcggcg ccggcgtcgt cgagcgctac
     2881 ctggaatgcc tggccgccca cgactgggac ggcctggcaa ccacgattgc cgacgaaggc
     2941 ctgatccgag agggaccctt ctgcgacgtc gtcgaaggca agcagcacta cgtcgcttat
     3001 ctgcgcaagg tcctcacgaa tcttgagggc caccggttgg aggtgcagcg ggtttcgcac
     3061 gtggattccc gggtgtccta cgtggagctg accgaggcat tcgagatcga cggagtcgcg
     3121 gcgacatggc ccgaatgcat cctcttcgaa ctgggcgacg acggcctgat ctgtcatgtc
     3181 agcgtgttct tcaagcagcg cggggccgac cgggccgccg atggggcctg agctgggcgg
     3241 atttccggct cgatcggaca cgccggggcg atatgggccg gagggagaac tattccctca
     3301 cccgtggaat tgacctaacg tgtgatgggt ggagtcacac ggaaccccag aattggtgcc
     3361 cgtcgaaaaa ttgcattcag gtgaccccgt caccgatgtc aacggtggcg gtcaacggta
     3421 catagttctc gagtcgaaaa ccctcggcga tagctgcgtg gtcctcgaac tcgagtccag
     3481 agtggaccat cagctgcagg ttatcgaaaa gtccttcccg accgggtacc acttcggtag
     3541 ggcgcaccac cgaattttgt aggcgattct cgccatctga ataccgcggt gcgtagctgt
     3601 tcgacggcta ccgcgcattc acgccaattg gcttgcgtag tgacttccgc aaagcaacgt
     3661 tttttgttgg gccgtcaatt tgggagcgct tcctgccggg tccgcgggct gcggccggca
     3721 tcgcaactgc ctcggtgacg cgccctcgtc gcccacgttt agacgtcccc gtaacccggt
     3781 aaccttcccg gataaccgag cgaatccgac ccgccacagt cgttggtgtc gacgcctgcc
     3841 gccctgttgg ggcagctgtg gacaggtcca tgacacgcca ggattattag gcgcggtaga
     3901 cgtaattgaa gggtgacgga atgaagattc cgggtgtagc cagcgtcgtc gttggcgtga
     3961 ccggcggggc cgcgcaggtg gtacgagcgg gtgtctccac tgcggcaggg gccgccggcg
     4021 ccgttcagac gttggccggc cccgtgttcg agcttgccgg cccggtggtc caatcgatgt
     4081 ctcaatccac gcgccgggca atcgggatcg gcgtttcccc cgacggctcc gctgaccgca
     4141 tcgcgccgcc ggtgcgctgg cagagcgggc ggcgagtgca cctggacctg gatcccttgc
     4201 tgccgtttcc caactggaac gagcatgcgg cggtggtgga ggagcccgtt cgcaggattc
     4261 cgggagtggc cgcggcccat gtcgagggcg cactcggccg gctggtgatc gaactcgaga
     4321 ccgagatcga cagcgccatg gtcctggacg acgtgcggga cacggtttgt tgcgtggccg
     4381 ccgatctcgc ctccgcggga ccaaagtcgg cgccccgcac cgcgccgttc gccgacccgg
     4441 gtaacccgtt ggcgatcctg gtcccgctca ccgccgcggc gatggatgcc gcggcgatcg
     4501 gcgccgcggt caccggctgg gtggtccggc tgcccgtcgc gccgcgaagc gcacgcgctg
     4561 ccgtcgctct gctgaatcat caaccgcggg tggtggccgt cctcgagtcc cggctgggcc
     4621 gcgtcggcac cgacatcgcg ctgagtgctt ccacggcgct ggccaacggg ctgacccagg
     4681 cagtcggcac gcccctgctg gacctggcgc aacgcggcct gcagatctcc gaggcggcgg
     4741 ctcatcgtga gcggtggcgc gagcgcgagc cggagctggc ctcgcctaag cgcccccaag
     4801 ctccggtggt tcccgtcatc tcgtcggcag gcgccaactc gcaggcaccc cggcacaact
     4861 gggcggccgc cgccgcgggt gacgtttcgc acgtcatggt cggtggggcc atcgacgccg
     4921 ccatcgacac agccaaggga tccatggccg gcccggtgga ggagtatgcc gaccaggccg
     4981 cgaacgcttc gttgatagcc gcggcgggtg cgttgctggc cggcggcggc accgaagacg
     5041 cggccggtgc gatcctggcc ggggtgccga gagccgcgca cgtgggccgg caggcgttcg
     5101 cctccgtgct cggccgaggc cttgccaacg ccgggcagct gatcctcgac cccggagcgc
     5161 tgcgacggct ggatcgggtg aaggtgatcg tgatcgacgg cgcggcgctg cgcggcgatg
     5221 cccgggccgt attggaggcg cggggagaca tccccggctg ggacgaggac cgggtttatg
     5281 aggtcgccga cgcgctgctg cacggcgagg aggcacccga tccggacccc gacgagctgc
     5341 cggccaccgg agcgcggctg cgatgggtgc gggcgcaggg gtcgtcggcg gcaccggccc
     5401 aaggtctcga acatgccgac ctcgtggtcg acggcgaacg cgccggcagc gtcgaggtgg
     5461 gatgggaagt cgaccccttc gccatcccgc tgctgcagac cgcgaaccgg accggggcgc
     5521 gggtggtgtt gcgccacgtt gccggcaccg aggacctgac gcccagcgcc gcggtgactc
     5581 atccacccgg cacgccgttg ctgaagttgg tgcgtgattt gcgcaccgat cgcggacccg
     5641 tgctgctgat cacgtcgctg cacccggact tcgcatcgac cgacaccctg gcggcactgg
     5701 cgatcgccga cgtcggggtg gccctcgacg atccgcacgc cgcgacaccg tggaccgcgg
     5761 acatcatcac cggcaccgat ctcgccgcgg cggtgcggat cttgtcggcg ctgccggtgg
     5821 cccgggacgc caccgaatcg tcggtgcgtc tcgccaaggg cggcagcact ctgtccgggt
     5881 tgttgctcgt cagcggtgag ccgcgctcgg ccaacccgat cgccttgcag cgctggctca
     5941 atccggtcaa cgccgccggc acggccgcat tgatatcggg agccttctcg gctagccggg
     6001 tgctccggtt gcccgatccc acgccgcaac cgctcaccgc ctggcacgcc ctggacccgg
     6061 agattgtcta cgcccggctg accagccgca cccgaccgtt ggccgtcgag gccggcgtca
     6121 cgccctggcg gcagttcctc gacgacctgt cctacaaccc ggccgtcgcg ccgctgcgcg
     6181 ggccggcgac ccaggtggtg cggctgctgg gtgcgacgcg ggccgagctc gccgatccgc
     6241 tgacgcccat cctggccgtg ggagcggcgg cctcggcgat cgtcggcagc agcatcgacg
     6301 cgctgctggt cgcgggcgtg atgaccgcca acgcgttggc cggcggggtg cagcggctgc
     6361 gggcggaggc ggcgatcgcc gagctgttcg ccgaccagga ccagcacgcg cgccgcgtcg
     6421 ttctcccgac ggttgctacg gcacgccgcc gcctggacgc ggctcgttcc accgaacgaa
     6481 cggtcacggt gagcgcgaaa tcgctgcggc cgggcgacat catcgacctg accgcaccgg
     6541 aggtggtccc ggccgacgca cgcctgctgg tcgccgagga cctggaagtc gacgagtcct
     6601 tcctcaccgg cgagtcgctg ccggtggaca agcaggttga gcccgtcgcc gtcgccgacg
     6661 ccgagcgggc cagcatgctg ttcgagggca gcaccattgt cgccggtcac gctcgcgcga
     6721 tcgtggtggc caccggtgcc ggcacggcgg cgcaacgcgc gatttcggcg atctccgatg
     6781 tcgagtcggc ggcgggcgtg caggcacggt tgcgtgagct gaccagcaaa gtgctgccgc
     6841 tcacgcttgc cggcggggcg gcggtgaccg gtttggcttt ggcgcaccgc acgtcgctgc
     6901 gccaagcggt cgccgacgga gtcgcgattg ccgtggccgc agtccccgag ggcctgccgc
     6961 tggtggcgac gctggcccaa ctcgccgccg cccagcgtct gtcgcggcgt ggggcgctgg
     7021 tccgcgcgcc ccgcgccatc gaagcgctgg gccgggtccg gacggtgtgt ttcgacaaga
     7081 ccggcaccct caccgagaac catctgcgcg tcgtttccgc agtgccgcac gggatcagcg
     7141 cgcagggctc gttccccgat gcggatgacc cgcgggccac cgcggtggtg cgcgccgccg
     7201 cccgggcctg cacgcagccc cacaacgggc agggacacgc gcacgccacc gacgaggcca
     7261 tcctcacggc tgccaattcc cttgacggtc aacgcaattc ggagtggacg gtgctcgcgg
     7321 aggtaccgtt cgagtccagc cgtggctact ccgcctcgat cggcgccgcg agcgacgaca
     7381 ccgctgggcc gatgttgatg gtcaaaggcg ctcccgagga gatcctgccc cgctgccggt
     7441 tcgccgatca ggaggcggac ctcgacgacg ccgaatcgtt gatattgcgc ctcgccgaac
     7501 aggggctgcg agtgctggcg gtggcacagc gccgctggga ccgcgggacc accgaggaag
     7561 acaccgacgc cgatgccgtc gacgccgctg cgcacgacct ggaattggtc ggctacgtcg
     7621 ggttggccga caccgcacgg ccgtcggcgc ggccactgat cgaggggctg ttggattctg
     7681 gccaccgagt ggtgctgatc accggcgatc accccgtcac cgcacgggcg atcgcccgcc
     7741 aattgggcct gccggcggat gcgcgcgagg cgaccggtgc cgaactggcg tccctcgatg
     7801 aggatgcgcg cgccaaactg gccgccgacg tgcaggtgtt cgcccgggtc agcccggagc
     7861 aaaaggtaca gattgttgcg gcgctgcagc agggcggcca ggttaccgcg atggtcggcg
     7921 acggcgccaa cgacgccgcg gcgatccgga tggccgacgt gggcatcggg gtgagcggtc
     7981 ggggttcgtc ggcagcgcgt ggggcggccg acatcgtctt gacggacaac gatctcggtg
     8041 tgctgctcga cgcgttggtc gaaggccgcg ggatgtgggg cggtgtgcgg gatgcggtgg
     8101 gcattctggt cggcggcaac gtgggtgaag tgctattcac catcatcggc accgcgctgg
     8161 gcaccggccg cgcgccggtg ggcacccgtc aactgctcct ggtgaacctg ctcaccgaca
     8221 tgtttcccgc gctggcggtt gccgtcacgc cccagtaccc ccagcccgaa gagaccgagg
     8281 atggggtcga tcgcgacgcc gaggagatgc agcgcgcatt ccagcttgcc gcgctggccg
     8341 aacccgcgcc gtcactggac gcgccgctca tgcgccagat cgtcacccgc ggtgccgtca
     8401 cggccgccgg cgctacggcg gcctgggcga tcggccgttg gacgccgggc accgaacgcc
     8461 gcacgtcgac aatgggtctc acggcactgg tgacgacgca gctcgcgcaa accctgctga
     8521 cgcgccgcca cagcccactg gtcttggcca cagccctggg cagcgcgggg gtcttggtcg
     8581 gcatcgttca aactccggtc atcagccagt ttttcggctg cacaccactg ggaccgatcg
     8641 cctggtccgg cgtgatcagc gccacggcgg gcgcaaccgc cgtctcggtg cttgcgccca
     8701 actggctggc caatagggtc gccgccctgg aacccaaaca ggactgagtc tgcgtcgctc
     8761 acagcgcacg aattacgtgc cattgcaatg cgtttcgacg ctacggtgcg gtctggtgcc
     8821 ggcctgatcg gcatggaatg ctgcaggcct gctgaaccgc gtggctaggt caacaactcc
     8881 ttgcgcacga ccttgccggc ggggttgcgt ggaatgccgg cgacgatgtg gatgtctctg
     8941 ggctgttcga agcgagaaac cttgccctgc aaatactccc gcaaggccga tgcgtcgatg
     9001 tcgcttcccg gctgtaggac cacgaacgcg gccagccgtt gaccgaaccg ctcgtcgagg
     9061 accccgatga cggcgttctc ggcgatgtcg gggtgttcgg caagcgcgtt ttcgaccgca
     9121 cgcggataga cgttctcgcc gccggagatg atcatgtcgt cttcgcgccc gacaatgaag
     9181 agccgacccg tgttgtcgaa atagcccatg tctccggtgc cggtcatgtt gtcgatcgtc
     9241 gccttcgcct ccccaacaat cgagccgccc gtgtaaccct cactcgtcat ctcgccaccc
     9301 acgaagatgc gtccggtaac ccgcgggccg acgggcctgt ctttcttgtc gaagatgcgc
     9361 acccggcccc cggcgaccgg ccgcccgacg gtttcgggag cgtcccgcag gtcggctggt
     9421 gttgcgaggg cgccgatacc gacctcggtc gagccgtagc cgttgaacag gatgtcgcca
     9481 taagcatcca tgaaccgctg ccccaggctg ggatcgagcc ggtccccgct ggacaacacc
     9541 acccgcaggt agggcagcgg gtttcgggcg cgcacccgct ggggcaggtc gaggatccgc
     9601 gcgagcacga tcgggaccac cgtgaacgcg tccgcgcgat gcagcgaggc ctgcgcgagc
     9661 gcggtttcgg cgtcgaagtg gcgacgggtg agcaccgtgc cgccgagggc cagtgtgagc
     9721 atcagcgtgc cgagcccgag cccgtgaaac atcggcatcg ccaccgaaat ccgcgacccg
     9781 gttcgcaacc gggtgcgatc gaggatcgtc acccagacgc ccaacgccga agcgatccgc
     9841 ggcgcgcgcg gcacgccttt gggcgtgccg gtggtgcccg acgtgagcag gacgatccgg
     9901 ccgggcgcgc tcactcgggg tcgcgagtct ccgtcgtggg catcggcgct cgccgggtcg
     9961 acgaccgccg acgcgtcggc ctcccgcaac cgtcccgcga attcgtcgtc ggcgaccgtc
    10021 gtcgtgatcc ggtgcgcgct caacgcggcc gccagcgcgt cgatccggaa ttcggtgttg
    10081 atcggcacaa cgtcggcgcc caccaggacg gcggcgaaga acgcccgcac gaagccgcgt
    10141 ccgttgcggc acatgacgcc caccgcgtga ccgggtccca cgccgtggcc ggagagctcg
    10201 cgggcgagcg attcgatgcc tgatcgcagc tcgcgatagg tcagcacgcc gtcgtcgtcg
    10261 atgatcgcgg ccctgttcgg ccaccgggcc gcc
//
LOCUS       DASSGQ010000283         3158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_232298,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000283 DASSGQ010000000
VERSION     DASSGQ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232298"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            543..2072
                     /gene="istA"
                     /locus_tag="VFI55_12435"
     CDS             543..2072
                     /gene="istA"
                     /locus_tag="VFI55_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_036335588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HET7074877.1"
                     /translation="MKSSEEIMEILEAYDLTGSLRGAATLAGCDHKTVAHYVALRDAG
                     VAPDQRPQRSMLIDEFLDKVEEWVDKSEGRIRADVVHGKLLAMGFTGSERTTRRAVST
                     ARRAWWSGHRRVFRPWVPEPGLWLQFDWGDGPRIGGRKTLLFCAWLAWSRYRVVIPTW
                     DRTLPTLLGCLDAALRCIGGAPTYALTDNEKTVTVEHIARVPVRHPDIVSAGRYYGMT
                     IRTCVPADPQSKGGAEATVRIAKADLVPTDANLRDDYATFAELVDGCEVFCTDVNGRP
                     HRVTGRPPVELLPEERQRLHVLPEHPVTVAFGQTRHVGWDATISVDGVRYSVPHQHVD
                     DRVWVRWAGEELVVTVIDPGGPREITRHQRGQKGRPQISDQHYPSGHPGRRALPGDRT
                     PKAVNPAEEAFLSIGDGAKAWLVEAAATGASRMRSKMAEAVTFAKLHSAAAVDQALGT
                     AALAGRFGDNDLASILAHQQAGPTGPATRASETHSLQPGTISWAGFGTPASIDSVGGE
                     A"
     gene            2069..2917
                     /gene="istB"
                     /locus_tag="VFI55_12440"
     CDS             2069..2917
                     /gene="istB"
                     /locus_tag="VFI55_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020106248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HET7074878.1"
                     /translation="MTAAKTAAGNTRANTTQAAVSVGPAVAAPSTQTLEKVIALTRRL
                     RLPYLRSAAVDVVPTARAQRWDPAELLRVLLTEEITGRDQATLRMRRRQANFPAGKTF
                     AVWDEARCSIPPPTQQALRSLEWIDRQENLCVCGPSGTGKSHFCEALGQLAIDSGRTV
                     AWFSIEELGALMRRHRADDSINKAIRRLTRVDLIIVDDIGMLAVGDDAAEGFYRLVDA
                     CYEKHSLAVSSNLHPSGFDEIMPKTLATATVDRLLHHAHICVTDGQSFRLAQATAGKG
                     VTPLPN"
BASE COUNT          541 a         1057 c         1069 g          491 t
ORIGIN      
        1 cgggccggta gagacacctc gctcgcagcg cgcggagctt gtcacggttc gtgacggcga
       61 tcgtgtatta ccgccacctt gagtgagagt ctggaaacga caacacggca cgaagggctc
      121 gacgaagacg ctgcacaggt acaagctcgc acaggcctgt caggttctgc tcgagggcaa
      181 gacgtcgccg acgtcaatca acgtcgtggt aacttggacc cggctggttg tgatagacga
      241 ctccaccatt ggtgatcacg cagcgtccgg aatatcagcc atcagcggtc cacgctgtcg
      301 cctgcactca gagttgatcg accctcgatc cacatggctc agttctcgga tccgacccgg
      361 atgtgacagc ggacgtggaa atctccccat gggcggccat cggatctccc cgctggcggt
      421 catgaaatcg acccagtgac ggccacggtt tgtccccgct tgtgccgtgt cgctgccggg
      481 ttccggtggc gctgttgggc tggctgatct cgaccgagcg tgtcgagtga ggggccggct
      541 tagtgaagag cagcgaggag atcatggaga ttctggaggc ttacgacctg accggcagcc
      601 tgcgtggcgc ggccacgctg gccggttgtg atcacaagac ggtggcgcac tacgtcgcgt
      661 tgcgtgacgc cggagtggcg ccggatcagc ggccgcagcg atcgatgctg atcgacgagt
      721 tcctggacaa ggtcgaggag tgggttgaca agtccgaagg caggattcgc gctgacgtgg
      781 tgcacgggaa gctgctggcg atgggattca ccggctcgga gcgcacgacc cgccgggcgg
      841 tgtcgacggc gcggcgggcg tggtggtccg gtcaccgccg ggtgttccga ccttgggtgc
      901 ccgagcctgg gttgtggttg cagttcgact ggggcgacgg gccgcgcatc ggcgggcgga
      961 agacgttgtt gttctgcgcg tggctggcgt ggtcgcgcta ccgggtggtg atcccgacgt
     1021 gggaccgcac cctgccgacg ctgctcggct gtttggacgc agcgctgcgc tgcatcggcg
     1081 gcgcacccac gtatgccctg accgataacg agaagacggt gacggtcgag cacatcgccc
     1141 gggtccctgt ccgccatccg gatatcgtgt cggccggccg ctactacggc atgacgatcc
     1201 gcacctgtgt gcccgccgac ccgcagagca agggcggtgc cgaggcgaca gtgcggatcg
     1261 caaaggccga cctggtgccg acggacgcga acctgcgcga tgactacgcg acgttcgccg
     1321 aactcgtcga cggctgcgag gtgttctgca ccgacgtgaa cggccgcccg caccgcgtca
     1381 ccggccggcc gccggtcgaa ctgctgcccg aggaacggca gcggttgcac gtgctgcccg
     1441 aacacccggt gaccgtggcg ttcgggcaga cccgacacgt cggctgggac gccaccatca
     1501 gcgtcgacgg cgtgcggtac tcggtgccgc accagcacgt cgacgaccgt gtctgggtgc
     1561 ggtgggccgg cgaggaactc gtcgtcaccg tcatcgaccc cggcgggcct cgcgagatca
     1621 cccgccatca gcgtgggcag aaggggcggc cgcagatcag cgatcagcac tacccgtccg
     1681 gccatcctgg ccgccgggcc ttgcccggtg accggacacc gaaggcagtc aacccagcgg
     1741 aggaggcgtt cctcagcatc ggtgacggcg cgaaggcgtg gctggtcgaa gccgccgcca
     1801 ccggggcgtc gcggatgcgg tcgaagatgg ccgaggccgt caccttcgcc aaactgcaca
     1861 gcgcggccgc ggtcgaccaa gcgctcggca ccgccgccct cgcggggcgg ttcggcgaca
     1921 acgacctcgc ctccatcctc gcccaccagc aggccgggcc gaccggcccg gccacacggg
     1981 ccagcgagac ccacagcctg caacccggca ccatcagctg ggccggcttc ggcacacctg
     2041 cctccatcga ctcggttggc ggtgaggcat gacggccgcg aagaccgccg ccggcaacac
     2101 gcgagcgaac acgactcagg cggcggtgtc ggtcgggcca gccgttgcgg cgccgtcgac
     2161 gcagacgctc gagaaggtga tcgcgttgac ccgccggctg cggctgccct atctgcgcag
     2221 cgccgccgtc gatgtcgtgc cgaccgctcg agcgcaacgg tgggatcccg ccgagctgct
     2281 tcgcgtcttg ctgaccgagg agatcaccgg ccgggaccag gcgacgctac ggatgcggcg
     2341 gcggcaggcg aacttccctg ccggcaagac gttcgctgtc tgggacgagg cccgctgctc
     2401 catcccgccg ccgacgcaac aggcgttgcg atcgctggag tggatcgacc gacaggagaa
     2461 cctttgcgtc tgcgggccgt ccggcactgg caaaagtcac ttctgcgaag cgctcggcca
     2521 gctcgcgatc gactccggtc gcaccgtcgc ctggttcagc atcgaagagc tcggtgctct
     2581 catgcgtcgt caccgcgccg acgactcgat caacaaagcg atccgccggc tcacccgcgt
     2641 cgacttgatc atcgttgatg acatcggcat gttggccgtc ggcgacgacg ccgctgaagg
     2701 gttttaccgc ttggtcgatg cctgttacga gaaacactcg ctcgcggtct caagcaacct
     2761 gcacccgtcc gggttcgacg agatcatgcc gaagacactg gcgaccgcga cagtcgaccg
     2821 actactgcac cacgcccaca tctgcgtcac cgacgggcag tccttccgac tcgctcaagc
     2881 cacagccggc aaaggggtga cgcccctgcc gaactgaccc accggggaga actcacgtcc
     2941 gtccatgggg agaaaccacg gccaccaacg gggagatctc ctgaccgccc acggggggat
     3001 cgtcatgacc gttgacacgc agcggcttcg atgaccgtca ggccggcaat caccaaaagt
     3061 ccagacccga accgcggtcg tcatacccat cggtgagaaa gggtcggcgc tcgcgggtac
     3121 aacgggtgtg tgtcacccga tcatccactt cggccacg
//
LOCUS       DASSGQ010000284         6467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_323912,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000284 DASSGQ010000000
VERSION     DASSGQ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323912"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFI55_12445"
     CDS             complement(<1..203)
                     /locus_tag="VFI55_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HET7074879.1"
                     /translation="MKLLLLTLTSLAAAIGMATPVRADSNDDAFLASLQAAGITYPDP
                     GRVIAAGRWVCQAVDQGTQMVDIV"
     gene            389..1129
                     /locus_tag="VFI55_12450"
     CDS             389..1129
                     /locus_tag="VFI55_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET7074880.1"
                     /translation="MSEPTPIAYPVRLDELIDAIKRVHTDALDQLADAVLAGEHLGEI
                     ADHLIGHFVDQARRSGASWTDIGKSMGVTKQAAQKRFVFRAEAAEIDPEQGYFGRFTP
                     RARNAVVFAQNAAHEAANAEITPDHLLLGVLSDPGALATVLLQRQGVDTETLRASVTL
                     PPAGPERPALIPFSGPARKALELTVREALRLGHNYVGTEHLLLALLELEDGAGPLHRS
                     GVDKDHVEADLIAALESLTGGKGAAQGG"
     gene            1182..1706
                     /locus_tag="VFI55_12455"
     CDS             1182..1706
                     /locus_tag="VFI55_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074881.1"
                     /translation="MRRWLIVLSTLLVAAAGAAFAGFGAPHAWAGDAPIGHIGDTLRV
                     DTGTYIADVTVSSVAPCDPPPGFGYTRSDVPVKSFPGSTVDRADVTIRAIRVPNPYIM
                     ATDFSFDGVTPFADAYKPRPSDAPDALDNVLVNAPNGAIVRGGVYWDAYRDPVSTVVL
                     LDKKTGYHLAQWNL"
     gene            1727..2260
                     /locus_tag="VFI55_12460"
     CDS             1727..2260
                     /locus_tag="VFI55_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7074882.1"
                     /translation="MAVRVVTTGSVDTDELAAVAARAFPLACPPSTPPENIASFIDAN
                     LSAERFAEYLADPRRAVLTAALDGRIIGYAMLIRGVGDDPDVARAVDIRPAAELSKMY
                     LLPDYHGAGVSRALMDHTLATAEDWSARCVWLGVNQANQRAQRFYAKSGFKISGTRTF
                     HVGARREHDYVMLRALA"
     gene            complement(2238..3338)
                     /gene="zapE"
                     /locus_tag="VFI55_12465"
     CDS             complement(2238..3338)
                     /gene="zapE"
                     /locus_tag="VFI55_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HET7074883.1"
                     /translation="MSLIAARRYSACMHGSTFGRVAHLVDRRPTVSPERLIAQLRPPP
                     TFADVSFATYRPDPAEPTQAAAVVAGEEFCRQAVERRAGRRKLFGKRVALPGVGVYLD
                     GGFGVGKTHLLASAYYRLPGDGPESPTPKAFATFGELTQLAGVFGFVECIDMLANYTA
                     VCIDEFELDDPGNTTLVSRMLSSLVERGVSVAATSNTLPEQLGEGRFAAQDFLREINT
                     LASIFTTVRIEGPDYRHRGLPPAPPPLSDGEVAERAAEVDGATLDDFDALCAHLATMH
                     PSRYLTLIEGVTEVFLTGVHAIDDQNVALRLVSLTDRLYDAGIPVVASGAKVDTIFSE
                     EMLAGGYRKKYLRATSRLVALTAAGIRQAREA"
     gene            3337..4119
                     /locus_tag="VFI55_12470"
     CDS             3337..4119
                     /locus_tag="VFI55_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020728436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine reductase family protein"
                     /protein_id="HET7074884.1"
                     /translation="MPDAGKAAEPSETQLTLLGSIRRLDDGELPELYGYPDRGATWVR
                     ANFITSVDGGATADGKSGGMAGPGDRLVFNLLRELADVIVVGAGTVRVEGYSGAHLGV
                     AERQRRQARGQREVPPLAIVTKSGRLDRDMPVFTRTEVPPLVLTCTAAADKTRRLLTD
                     LCEVVDCSGSDPAEVDEAALLTALAARGLRRVLSEGGPTLLGAFIERGLLDELCLTIA
                     PYVVGGLARRIATGPGQLLTRMRCAHILTDDAGYLYTRYVKA"
     gene            4149..5723
                     /locus_tag="VFI55_12475"
     CDS             4149..5723
                     /locus_tag="VFI55_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7074885.1"
                     /translation="MTRQGRSATVLGAVAFSATLVLTGCVPGLAADPRFATNSGARPQ
                     GVPPTKHPPSGPPPIAAPKNDLAWHDCTSRVFTDAAVPAAAGVQLDCANYDADLDPVN
                     GGSGTVSIGVVRARSNQTPKDAGPLVFTTGSDLPSSAQLPVWLSGAGADVLQTHPIVA
                     VDRRGIGMSTPIDCVNHSDRQVMRDQAQFQGGDDPVANLSEVSDTATTNCTDALAPAG
                     DAAYDNSHAASDIERLRNLWDVPAVALIGIGNGAQVALAYAASRPDKVARLILDSPIA
                     LGVNAEAAAEQQVKGQQAALDAFAAQCVAVNCALGPDPEGAVSALLADARAGRGPGGA
                     SLAAVANAITVALGFPVGGRVNATTSLAGALAAARSGDAGQLNNLINRAYATAGSDGQ
                     FVNSCSDAINRPTPDRVRELVVAWGKLYPQFGTVAALNLVECVHWPTGSPPAPPKDLK
                     VDVLLAGVLNDPIVGVEGVAATAATIINANAASKRVMWQGIGHGASIYSPCAVPPLIG
                     YLNSGKLPATDTYCPA"
     gene            5754..>6467
                     /gene="aftC"
                     /locus_tag="VFI55_12480"
     CDS             5754..>6467
                     /gene="aftC"
                     /locus_tag="VFI55_12480"
                     /EC_number="2.4.2.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinofuranan 3-O-arabinosyltransferase"
                     /protein_id="HET7074886.1"
                     /translation="MYGALVTAADSTRTGLRDWILAAFRPRTSAPSAATVLRSVLWPL
                     AIMSVLHRSIVLPVNGNITDDFKPVYRAVLNFRRGWDIYNEHFDYVDPHYLYPPGGTL
                     LMAPFGYLPFTPSRYLFILINAVAILIAWYLLLRLFKFTLSSVAAPALLLAMFCTESV
                     TSTLVFTNINGCVLLAEVLFLGWLLDGSQTNRVGHQWWAGIVIGLTLTLKPVLGPLLL
                     LPLLNRQWRALVPAFVVPAV"
BASE COUNT          999 a         2339 c         2118 g         1011 t
ORIGIN      
        1 acgatgtcaa ccatctgcgt gccctgatcc accgcttggc acacccatct gccggccgcg
       61 atgacccggc cggggtcggg gtacgtgatg cctgccgcct gcagtgacgc gaggaatgcg
      121 tcatcgttgc tgtcggcgcg cacgggggtg gccatgccga tcgcggcggc aaggctggtc
      181 agcgtcagga gcagcagttt cacggtccca tgatcgtgcc agaatggcgg ccggcccgca
      241 tctcttttgc tggtccggat attcgctgcg aaaggaactg cgcgtcaagg tcattcggag
      301 cgcgccggag ctcccgcgag cgctggatcg agcaaacagc gtcaacccaa ggttgacacg
      361 tagcggtcgt caacctagtg ttgacactat gtccgagccg acacccatcg cctatccagt
      421 ccgcctagac gagctgatcg acgccatcaa acgcgtgcac accgacgcgc tcgaccaact
      481 cgccgatgcg gtgctggccg gcgagcatct gggcgagatc gccgaccatt tgatcggtca
      541 tttcgtcgat caggcccgcc gctcgggagc ctcgtggacc gacatcggca agagcatggg
      601 cgtcaccaag caggccgccc agaagaggtt cgtgttccgc gccgaggccg ccgagatcga
      661 tcccgagcag ggctacttcg gccgcttcac gccgcgagcc cgcaacgccg tggtcttcgc
      721 gcagaatgcc gcccacgaag ccgccaacgc cgagatcacg cccgaccacc tgctgctcgg
      781 ggtgctcagc gatccgggag cgctggccac cgtgttgctg caacgacagg gcgtcgacac
      841 cgagaccttg cgcgcgtccg tcacgctacc cccggccggc cccgagcgac cggcgctcat
      901 tccgttcagc ggcccggcgc gcaaggcgct cgagctgacc gtccgcgagg cgctgcggct
      961 cggccacaac tacgtcggca ccgaacatct gctgctggcg ctgctcgaac tcgaagacgg
     1021 tgccggaccc ctgcatcgat ccggcgtcga caaggaccac gtcgaggccg acctgatcgc
     1081 cgcgcttgaa tcgctgaccg gcggcaaggg cgccgcccag ggcggctgag ccctcatccg
     1141 acccctcccc cacgtgttag gccgtgtgca atcatgcgaa ggtgcgccgc tggctgatcg
     1201 tcctgtccac cttgctggtt gcggctgctg gcgccgcttt tgccggcttc ggcgcccccc
     1261 acgcgtgggc gggcgacgcg ccgatcggcc atatcggcga caccttgcgg gttgacaccg
     1321 gcacctacat cgccgacgtc accgtcagca gcgtggcgcc gtgcgacccg cccccgggat
     1381 tcggctacac gcgcagcgac gtcccggtga aaagctttcc gggcagcaca gttgaccggg
     1441 ccgacgtgac gatccgcgcg atccgggtgc ccaatccgta catcatggcc accgacttca
     1501 gcttcgacgg ggtgactccg ttcgccgacg cctacaagcc gcggccatcc gatgcgccgg
     1561 acgcgctgga caacgtgctc gtcaacgcac cgaacggcgc catcgtgcgc ggcggagtct
     1621 attgggacgc ctaccgcgac ccggtctcca ccgtcgtgct gctggacaag aagacgggct
     1681 accacctcgc gcagtggaac ctgtgatccc cgcggaatct gcgactctgg ccgtccgcgt
     1741 cgtcacgacg ggttcggtcg acaccgacga actggcggcc gtcgccgcgc gcgcctttcc
     1801 gttggcgtgt ccgccctcga caccgccgga gaacatcgcg tcgttcatcg acgccaacct
     1861 gtcggctgag cgcttcgcgg aatatctggc cgatccgcgg cgcgcggttc tcaccgctgc
     1921 gctggacgga cgaatcatcg gttatgccat gctcattcgg ggcgtcggcg atgatccgga
     1981 cgtggcgcga gccgtcgaca tccgcccggc cgccgagctg tcgaagatgt atctgctgcc
     2041 cgactaccac ggcgccggag tgtcgagggc gctgatggac cacacgctgg ctaccgccga
     2101 agactggagt gcgcgctgcg tgtggctggg cgtcaaccag gcaaaccaac gcgcacaacg
     2161 cttctacgcc aaaagcggtt tcaagatcag cggcacccgg acgtttcacg tgggcgcccg
     2221 ccgcgagcac gactacgtca tgcttcgcgc gcttgcctga tccccgcggc agtcagcgcc
     2281 accaatcgtg acgtggcccg cagatacttc ttgcggtacc caccggccag catctcctcg
     2341 ctgaagatgg tgtccacctt cgccccggag gccaccaccg ggatgccggc gtcgtagagc
     2401 cggtcggtca gcgacaccag cctcagcgcg acgttctgat cgtcgatggc gtgcaccccg
     2461 gtcaggaaca cctcggtcac gccctcgatc agcgtcagat accgggacgg atgcatggtg
     2521 gcgaggtgcg cgcacagcgc gtcgaagtcg tccaacgtcg ccccgtcgac ctccgcggca
     2581 cgctcggcta cttccccgtc cgacaacggc ggcggcgccg gcggcaggcc gcggtgccgg
     2641 tagtcgggtc cctcgatgcg cacggtagtg aaaatgcttg ccagcgtgtt gatctcgcgc
     2701 aggaagtcct gagcggcgaa gcgcccctcg ccgagctgct cgggcaaggt gttcgaggtg
     2761 gcggccaccg acaccccccg ctcgaccaac gacgagagca tccgcgacac cagggtcgtg
     2821 ttgcccgggt cgtcgagctc gaactcgtcg atgcacaccg cggtgtaatt ggccagcatg
     2881 tcgatgcatt cgacgaagcc gaacacgccc gcgagctggg tgagctcccc aaatgtggcg
     2941 aacgccttgg gagtcgggga ctcggggccg tcaccgggca accggtagta ggccgaggca
     3001 agcaggtgcg tcttgcccac gccgaagccg ccgtccagat acacccccac cccgggcagt
     3061 gctacccgtt tgccgaacag tttgcggcgg cccgcccggc gttccacggc ctgccggcag
     3121 aactcctcac ccgccaccac ggcggccgcc tgggtcggtt ccgccggatc ggggcgatag
     3181 gtcgcaaagc tgacgtcggc gaacgttggc ggcggccgca attgagcgat caggcgctcc
     3241 ggcgataccg tcggtcgccg atccaccaga tgagccaccc ggccgaaagt ggacccgtgc
     3301 atgcaggcac tgtagcggcg tgctgcaatc aagctcatgc ctgacgccgg caaggccgct
     3361 gagccctcgg agacgcagct gacgctgctc gggtcgatcc gacgactcga cgacggcgaa
     3421 cttcccgagc tctacggcta tccggatcgg ggcgcgacgt gggtgcgcgc gaacttcatc
     3481 accagcgtgg acggcggcgc caccgcggac ggcaagagcg gcggcatggc cgggcccggc
     3541 gaccggcttg tcttcaacct gctgcgtgaa ctcgccgacg tcatcgtcgt cggcgcgggc
     3601 accgtgcggg tcgagggata ttccggcgcg cacctgggtg tcgccgagcg gcagcggcgg
     3661 caggcccgcg gacaacgcga agtcccgcca ctggcgatcg tcaccaagtc gggtcgcctc
     3721 gaccgcgaca tgccggtgtt cacccgcacc gaggtgccac cgctggtcct cacctgcacg
     3781 gccgcggccg acaagacccg ccgcctgctc accgacctgt gcgaggtggt cgactgctcg
     3841 ggcagcgatc ccgccgaggt ggacgaggcc gccttgctga cggcgctcgc ggcgcgcgga
     3901 ctgcgccgcg tcctgagcga aggcgggccg acgctgctcg gcgcgttcat cgagcgcggc
     3961 ctgctcgacg agctgtgcct gacgatcgcg ccctacgtcg tcggcggcct ggcccggcgc
     4021 atcgcgaccg gtccgggtca gttgctgacc cggatgcgct gcgcgcacat cctgacggac
     4081 gacgcaggct acctctacac ccgctacgtc aaggcctgat ccacggggcg acggctaggg
     4141 tggtccgcat gactcggcag ggcaggtccg ccacggtcct gggcgcggtc gcgttctcgg
     4201 cgaccctggt gctgaccggt tgcgttccgg gcctggccgc ggacccgcgc ttcgccacca
     4261 attccggtgc ccggccgcag ggggtgcccc cgacgaagca cccgcccagc ggtccgccac
     4321 cgatcgcggc gcccaagaac gacctggcgt ggcacgactg cacctcgcgg gtcttcaccg
     4381 atgcggccgt gcctgccgcg gccggcgttc agttggattg cgcgaactac gacgccgacc
     4441 tcgacccggt caacggcgga tccgggacgg tgagcatcgg cgtcgtccgc gcccggtcga
     4501 atcagacccc caaggacgcg ggcccgctgg tcttcaccac cggctccgac ctgccgtcct
     4561 cggcgcagtt gccggtctgg ctgtccgggg cgggcgccga cgtgctgcaa acccacccca
     4621 tcgtcgccgt cgaccgccgc ggcatcggca tgtcgacccc gatcgactgc gtgaaccact
     4681 ccgaccgcca ggtgatgcgc gatcaggcgc agttccaggg cggcgacgat ccggttgcca
     4741 acctctccga ggtctccgac accgccacca ccaactgcac cgacgccctg gcgccggcgg
     4801 gcgacgccgc gtacgacaat tcgcacgcgg cctccgacat cgagcggctg cgcaacctct
     4861 gggacgtgcc cgcggtcgca ttgatcggga tcgggaacgg tgcccaggtg gcgctggcct
     4921 atgccgcgtc tcgccccgac aaggtggccc ggctgatcct cgactccccg attgccctgg
     4981 gcgtcaacgc cgaagccgcc gccgagcagc aggtcaaagg ccagcaggcc gcgctcgacg
     5041 cgttcgccgc ccagtgtgtt gcggtgaact gcgcgctggg gcccgacccg gagggcgccg
     5101 tcagcgccct gctggccgac gcccgcgccg gcaggggacc cggcggcgca tcgctggccg
     5161 cggtggccaa cgccatcaca gtcgccctgg gattccccgt aggcggccgc gtcaacgcca
     5221 ccaccagcct ggccggcgcg ctggcggccg cccgctccgg cgacgcgggc cagctgaaca
     5281 acctgatcaa ccgcgcctac gccaccgccg gctccgacgg ccagttcgtc aactcctgca
     5341 gcgacgcgat caaccgcccg accccggacc gggtgcgcga actcgtcgtg gcgtggggca
     5401 agctgtatcc ccaattcggc accgtcgccg cgctcaatct ggtcgaatgc gtgcactggc
     5461 ccaccggttc gccgccggcg ccgcccaagg atctcaaagt cgacgtcctg ctggcgggtg
     5521 tgctgaacga cccgattgtg ggcgtcgaag gagtcgccgc gacggcggcc accatcatca
     5581 acgccaacgc ggccagcaag cgggtgatgt ggcagggcat cggccacggc gccagcatct
     5641 actcgccctg tgccgtcccg ccgctgatcg gctacctcaa cagcggcaag ctgccggcga
     5701 ccgacaccta ctgtcccgcc tgagacttgg gtttttgggc gctggtgcgc tcggtgtacg
     5761 gtgcgctggt gacggcagct gactcgaccc gaaccggctt gcgcgattgg atactcgccg
     5821 catttcgtcc ccgcaccagc gcaccgagtg cggctacggt gttgcggtcg gtgttgtggc
     5881 cgctggccat catgtcggtg ctgcaccgca gcatcgtgct ccccgtgaac ggcaacatca
     5941 ccgacgattt caaaccggtc taccgcgcgg tgctcaactt ccggcgcggg tgggacatct
     6001 acaacgagca cttcgactac gtcgacccgc actacctcta cccgcccggc ggcacactgc
     6061 tgatggcgcc gttcgggtat ctgcccttta cgccatcgcg atatctgttc atcctgatca
     6121 acgccgtggc catcctgatt gcctggtacc tgctgctgcg tttgttcaaa ttcacgctgt
     6181 cgtcggtggc cgcgcccgcc ctgctgctgg ccatgttctg cacggagagc gtgaccagca
     6241 cgctggtgtt caccaacatc aacggctgcg tcctgctggc cgaggtgctg ttcctcgggt
     6301 ggctgctgga cgggtcccaa acaaaccgag tcgggcacca gtggtgggcc ggcatcgtga
     6361 tcgggctgac gctcacgctc aaacccgtgc tgggcccgct gctgttgctg cccctgctga
     6421 accggcagtg gcgggcgctg gtgcccgcgt tcgtcgtgcc cgcggtc
//
LOCUS       DASSGQ010000285        12541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_901432,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000285 DASSGQ010000000
VERSION     DASSGQ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12541
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901432"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="VFI55_12485"
     CDS             complement(<1..116)
                     /locus_tag="VFI55_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgA"
                     /protein_id="HET7074887.1"
                     /translation="MGESSLNPTLLRRISTSLRPDWTRTVLARRVAAAGLVG"
     gene            complement(182..553)
                     /locus_tag="VFI55_12490"
     CDS             complement(182..553)
                     /locus_tag="VFI55_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HET7074888.1"
                     /translation="MPTYSYACTECDNRFDIVQGFADDALTTCEKCSGRLRKLFNSVG
                     IVFKGSGFYRTDSREDGKKSKSSVNGSSTSDAGSSSGSSEKNGSSEKSGSSEKSGSSE
                     KSGSSEKSSSAATPSTAAATT"
     gene            complement(627..1241)
                     /locus_tag="VFI55_12495"
     CDS             complement(627..1241)
                     /locus_tag="VFI55_12495"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET7074889.1"
                     /translation="MATASKSALRRQLLAARHAVADEVRAAEAHMLTDHLESALTSGS
                     TVCAYVPVGTEPGSIEMLDTLLRRSGRVLLPVARTAGGGTRDMPLPLRWAEYRPGTLI
                     AGPWGLLEPPGPVLPEPALAEASLVIVPALAVDRRGVRLGRGRGFYDRSLDGRDPQAR
                     LIAMVRDAEFVDELPAEAHDVPMTHVITPRRGLIALLPAAHSGE"
     gene            1319..2230
                     /locus_tag="VFI55_12500"
     CDS             1319..2230
                     /locus_tag="VFI55_12500"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HET7074890.1"
                     /translation="MSHPGVPIPYTAIVPAAGLGTRFLPATKTVPKELLPVVDTPGIE
                     LVAAEAAAAGAERLVIVTSEGKDGVVAHFVEDLVLEGTLEARGKKAILAKVRRAPALI
                     KVESVVQAEPLGLGHAISCVEPTLAADEDAVMVLLPDDLVLPSGVLETMSKVRAHRGG
                     SVLCAIEVTPDEISAYGVFDVEPAADGDDPGVLKVKGMVEKPKTEDAPSMYAAAGRYV
                     LDRAIFDALRRIDRGAGGEVQLTDAIALLIQDGHPVHVVVHRGSRHDLGNPGGYLKAA
                     VDFALDRDDYGPDLRRWLVARLGLTEH"
     gene            2387..3679
                     /gene="glp"
                     /locus_tag="VFI55_12505"
     CDS             2387..3679
                     /gene="glp"
                     /locus_tag="VFI55_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HET7074891.1"
                     /translation="MRSVEEQQTRISAAAVAPRPMRIAIAEAQGLMCAEEVVTERPMP
                     GFDQAAIDGYAVRSVDVLGVGEVGEVGGFGESSDDTGGDEDRREGLTLPVMGTIEAGT
                     RTPSRLQPRQAVRVQTGAPLPTLADAVLPLRWTDGGTSHVRILRGAPSGAYVRRAGDD
                     VQPGDVAVRAGTVIGAAQVGLLAAVGRERVLVHPRPRLSVMAVGGELVDIARTPGNGQ
                     VYDVNSYALAAAARDAGAEVNRIGIVSNDPNELREVVEGQISRAEVLVIAGGVGGAAA
                     ESVRSVLSALGEMEVVRVAMHPGSVQGFGQLGREGVPTFLLPANPVRALVVFEVMVRP
                     LIRLSLGKRQPMRRIVQARALSPITSVAGRKGYLRGQLMRDQDTGEYLVQALGGAPGA
                     SHLLATLAEANCLVIVPSGAEQIRTGEIVDVAFLAQRG"
     gene            3701..4354
                     /locus_tag="VFI55_12510"
     CDS             3701..4354
                     /locus_tag="VFI55_12510"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET7074892.1"
                     /translation="MNLLRSNSRHPGWPQDVGPLRVAAGLVRLRTVRMRDGAQWSRIR
                     LADRAHLEPWEPSTDGDWTVRHTVAAWPALCSGLRAEARKGRMLPYVIELDGRFCGQL
                     TIGNVTHGALRSAWIGYWVPRSATGGGVATAALALGLDHCFGPVMLHRVEATVRPENA
                     ASRAVLAKVGFREEGLLRRYLEVDKAWRDHLLMAITVEEVHGSVASALVRSGHAGWA"
     gene            4548..5627
                     /gene="glpR"
                     /locus_tag="VFI55_12515"
     CDS             4548..5627
                     /gene="glpR"
                     /locus_tag="VFI55_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014588187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase receptor GlpR"
                     /protein_id="HET7074893.1"
                     /translation="MPSIPQSLLWISLVVLWLFVLVPMLISKRDAVRRTSDVALATRV
                     LNNGAGSRLLKKRTGPAAGHRSDPHWKAEEDPAEDELADAQQVHRDDVHEDHDQEDDT
                     EEVQSAPPVVMKVAVNERKEPDYLDVEVVEDSGALPAGASATAAEPGSDDIDQEGVDA
                     ESVSVGQHGEGADAAADRGDEYEYVEDSSGLEPEADRDEGEDEDARAFVASGSSRRRR
                     FDTKTAAAVSARKYAFRKRVLMAMAVILVGSATAAFEVSSTAWWFCGTATAMTMVYLA
                     YLRRQTRIEERVRRRRMQRMARARLGVENTYDRGQDVVPSRLRRPGAVVLEIDDEDPI
                     FEHLDNAMPMRTFGWPRDLPRAVGE"
     gene            5678..5750
                     /locus_tag="VFI55_12520"
     tRNA            5678..5750
                     /locus_tag="VFI55_12520"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5711..5713,aa:Ala,seq:cgc)
     gene            6276..7436
                     /locus_tag="VFI55_12525"
     CDS             6276..7436
                     /locus_tag="VFI55_12525"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET7074894.1"
                     /translation="MTSRRQDPSIAPLRSAGLIDLNDLPIGLIDLELSAPPTGLVGET
                     AAFLLRPEQGPPAAVLKVARGTLKASELRTQRRVLTELAIHQGLDDEWRGLLPRILAF
                     DERADATVSVESYRPGTDLAEVLARRPNRVEELTTAALRAIAPLHRRTATSIVVDNVC
                     LLRRWVVEPLAGLTEMCRRLDPRMVPHLEGLGTMLRQDLVGRRMPVSWTHGAFTPENV
                     RVGGVRGPVTGIVDWAGARPGQPALIDEYLMILTASCQVEQADLGTVVAERIRAGGLS
                     ESERNALGAARDGSDGDAGERGAVDEGIAILLTWLHHAADLWRRSAIHPNHHTWWATN
                     VTPVIDAVVASDAFDVSSGRARSGRRAASVTSMTASPPAEGGAQAMGNQANG"
     gene            7553..8530
                     /locus_tag="VFI55_12530"
                     /pseudo
     CDS             7553..8530
                     /locus_tag="VFI55_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013827964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            8665..9648
                     /locus_tag="VFI55_12535"
     CDS             8665..9648
                     /locus_tag="VFI55_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7074895.1"
                     /translation="MRTLVTGAAGFIGSTLVERLLQEGHQVTGVDNLSAAPPVANLAS
                     AHRHKGPGRFNFVAVDVRHPELADIIVGSNPDVVFHLAAQVDVGCSVSDPQFDARNNV
                     LGTINLLEASRRAGVTRIVYAASGGSRYGAPATLPATEETAVDPLSPGAAAKVAGEFY
                     MRAYAGMYGLEPICLALANVYGPRQVPRGEAGVVAVFSTAMMSGRPTTIYGDGSATRD
                     YVYVDDVVTAFLCAAEAPDMTTGTFNIGTGVQTTVNELHDMIAAAVGVAHPPIHAAAR
                     TGEVRVSALDPTLAGQVLGWKPDIDLAEGIQRTVGWLRGRLDRQPAALARV"
     gene            10022..10420
                     /locus_tag="VFI55_12540"
     CDS             10022..10420
                     /locus_tag="VFI55_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7074896.1"
                     /translation="MERRRSGRSGESPTDILTRLPAVVVLERVPVPTLAMARDGMILF
                     ANTAFADMVGYEQDTLAGLAFPEIFHTVPAEVAALSGVEALANLVVELRHCEGWTVRA
                     RMSKSLMRRDDPVVLVTFENLTERLWVDEH"
     gene            11105..>12541
                     /locus_tag="VFI55_12545"
     CDS             11105..>12541
                     /locus_tag="VFI55_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7074897.1"
                     /translation="MAHIAFPDTDVSVLDADDLGDVVADFTAAAQDRAGRPAIVHNGT
                     VVTYEDLAERVGLAASRYRACRFDGDGPSGLIGVLVSHTPAVVEHLLGILQARATYCP
                     IDATLPVARKQALAAVLGLDRLFATAPDQIEQSSLRIETLDQADVPGDTDLPQPLWHR
                     SDPAYVLCTSGSTGAPKPVVVSRHAVTSTVRALRDLFALTPEDRVLQFASLGWDTCLE
                     EILPALTAGATLVFDNEAHSGSMPRFVRMLAEREITVLDLPTAFWHELVLFLDEERAA
                     LPDSVRLVVIGGERVDPTRLRQWRDLGTGHVRLLNTYGCTETTMITHAVQLAGPGTEP
                     ELAAQVDAPLGRPLPHVRDHVTDEGELLISGPSIATGYLGMPEVTAKGFPVADYGSGP
                     TRWFRTGDVVTRGERGLVYPRGRADEQVKVRGVRVHPAEVEAQLNTHPAVAGAVVVGE
                     RLVGHTSLTAYVVAAATTTPAELRRYLSE"
BASE COUNT         2010 a         4018 c         4310 g         2203 t
ORIGIN      
        1 aacccgacca gcccggccgc ggcgacacgc cgcgccagca cggtgcgagt ccagtccgga
       61 cgcaacgatg tcgatatccg gcgcaatagc gttggattca gcgatgattc gcccacgccg
      121 caacggtaag cgcagcgcag ctccggcgat tccgggcagg gattccgttg tggataactg
      181 tttacgtcgt ggcggccgca gtggacggcg tggcggcact gctcgacttt tcgctggagc
      241 cgctcttctc actggagccg ctcttctcgc tggagccgct cttctcgctg gacccgttct
      301 tttcgctcga tcccgaactc gacccggcgt cgcttgtcga ggacccgttg accgagctct
      361 tcgacttctt gccgtcctcg cggctgtcgg tgcgatagaa gccgctgccc ttgaatacga
      421 tcccgaccga gttgaaaagc ttccgcagcc gaccggagca cttctcgcac gtggtcagcg
      481 catcgtcagc gaagccctgc acgatgtcga agcggttgtc gcactcggtg cacgcgtagc
      541 tataggtcgg cacaaaaacc tccggatgat tccagccttg ttagcactct actttctcaa
      601 gtgctaaaac cgccaggtga ggcatctcat tccccgctgt gtgcggcggg gagcaaagcg
      661 atcaggccac gccgtggcgt gatcacgtgg gtcatcggca cgtcgtgcgc ctccgcgggc
      721 aactcgtcga cgaactccgc gtcgcgcacc atcgcgatca gacgcgcctg cgggtcccgg
      781 ccatccagcg agcggtcgta aaatccacgg ccccggccca gccgcacccc gcggcggtcg
      841 actgccagcg ccgggacgat caccagactg gcctcggcca gcgccggctc gggcagcacg
      901 gggccgggcg gctcgagcag cccccaaggc ccggcgatga gtgtgcccgg gcggtattcg
      961 gcccaccgca gcggcaaggg catgtcgcgg gtgccgccgc cggcggtacg cgccaccggc
     1021 agcaacaccc gtcccgaccg ccgtagcaac gtgtcgagca tctcgatgga ccccggctcg
     1081 gtgcctaccg gaacgtaggc gcacaccgtg ctgccgctgg tcaacgccga ttccaggtgg
     1141 tcggtgagca tgtgcgcctc ggcggcacga acctcgtcgg caacggcgtg acgggccgcc
     1201 agcagctgtc ggcgcagggc ggacttgctc gcggttgcca tgcctcaacc atgacaggcc
     1261 cggctttcgc gtggccatat gcagtcggcc ggggctgcgg cgcattatcg tttgaacaat
     1321 gtcacaccca ggagttccga tcccctatac ggcgatcgtc cctgcggccg gcctgggcac
     1381 gcggttcctc cccgcgacca agactgtgcc caaggagctg ctacccgtcg tcgacacgcc
     1441 cggcatcgag ctggtcgccg cggaggcggc tgccgccggt gcggaacgtc tggtgattgt
     1501 cacctcggag ggcaaggacg gcgtcgtcgc gcatttcgtc gaagacctgg tgctcgaggg
     1561 cacgctggag gcgcggggaa agaaagccat cctggccaag gtgcgccgcg cgcccgcgct
     1621 gatcaaggtc gagtccgtgg tgcaggccga gccgctcggg ctgggacacg ccatcagctg
     1681 cgtcgagccc accctcgcgg cggacgagga cgccgtcatg gtgctgctgc ccgacgactt
     1741 ggtgctgcct agcggcgtcc tggagacgat gtcaaaggtg cgggcccacc gcggcggctc
     1801 ggtgttgtgt gccatcgagg taactcccga cgagatcagc gcgtacgggg ttttcgatgt
     1861 cgagccggcc gccgacggcg acgatcccgg tgtgctgaag gtcaaaggca tggtggaaaa
     1921 gcccaagacg gaggacgctc cgtcgatgta tgcggcggcg ggccgctatg tgctcgaccg
     1981 cgccatcttc gacgcgttgc gccgcatcga ccgcggggcc ggtggcgaag tccagctcac
     2041 cgatgcgatc gcactgctga tccaggatgg ccacccggtg catgtggtcg tgcatcgcgg
     2101 atctcgacac gacttgggaa atcctggcgg ctacctcaag gctgcggttg actttgcatt
     2161 ggaccgtgac gactatggcc cggatttgcg gcgatggctg gtggcgcgat tgggcctgac
     2221 cgagcactaa cacagacgac cacgtcgtgc cggccgacat tgcgggcctg cgcagatcgc
     2281 gacggccccg gggtggggga tggtcccgcc cacggggagc gggctgaacg ggcccgggtc
     2341 gcccgagacg gcgctcgcta ggactggtgg gcagagaggc gcgcttgtgc gttctgtgga
     2401 ggagcagcag acccggatat cggccgccgc ggtggcccca cgaccgatgc gtatcgcgat
     2461 cgccgaggcg cagggactga tgtgcgccga ggaagtggtg accgagcgtc cgatgcccgg
     2521 gttcgatcag gccgcgatcg atggctacgc ggtgcgcagc gtcgacgtgc tgggtgtcgg
     2581 cgaagtgggc gaggtcggcg gttttggcga atcgtccgac gacaccggcg gtgatgaaga
     2641 ccggcgcgag ggtctgaccc tgccggtgat gggaaccatc gaggccggta cgcgcacacc
     2701 cagccggctg cagccgcgtc aggcggtccg agtgcagacc ggtgctccgc tgcccacact
     2761 ggccgacgcg gtcctgccat tgcgctggac cgacggcggg acgtcgcacg tgcggattct
     2821 gcggggtgcg ccctcgggtg catatgtgcg ccgcgcgggc gacgacgtcc agcccggtga
     2881 cgtcgccgtg cgtgccggca cggtcatcgg cgccgcgcag gtcgggctgc tggcggcggt
     2941 cgggcgcgaa cgggtcttgg tgcatccgcg tccgcgattg tcggtaatgg ccgtgggtgg
     3001 cgagctggtc gacatcgcac gcaccccggg aaacggccag gtgtacgacg tcaactccta
     3061 tgcgctggcc gcagcggccc gggacgccgg cgccgaggtc aaccgtatcg gcatcgtcag
     3121 taacgatccc aacgagctcc gcgaggtcgt cgaaggccag atcagccgcg cagaggtcct
     3181 ggtgatcgcc ggtggggtcg gcggtgcggc cgccgagtcg gtgcgttcgg tgctgtccgc
     3241 tcttggcgag atggaggtcg tccgggtagc catgcacccc ggatccgtcc agggcttcgg
     3301 gcagttgggc cgggagggtg ttccgacctt tctgttgccg gccaatcccg tccgcgcgct
     3361 ggtggtcttc gaggtgatgg tccggccgct gatccggctg tcgctgggca agcgccagcc
     3421 gatgcgcagg atcgtgcagg cccgcgccct gtcgccgatc acctccgtcg ccgggcgcaa
     3481 gggatatctg cgcggtcagt tgatgcgtga ccaagacacc ggtgagtatt tggtgcaggc
     3541 actgggtgga gccccggggg cttcacactt actggcgacc cttgccgagg cgaactgtct
     3601 ggtcatagtt cccagcggtg ccgagcagat tcgcaccggt gagatcgtcg acgtcgcctt
     3661 cctggctcag cgcggctgag ccaaacgccc gcacccgctc gtgaacctgt tgcgctcgaa
     3721 ttcccgtcat ccgggttggc cccaggacgt ggggccgctg cgggtcgcgg cggggctcgt
     3781 ccggttgcgc acggtgcgga tgcgtgatgg cgcgcagtgg agccggatcc gcctggccga
     3841 tcgtgcgcat ctcgaaccgt gggagcccag cacggacggt gactggacgg tccggcacac
     3901 cgtggccgcg tggccggcgc tgtgttcggg gcttcgtgcc gaggcccgca aggggcgcat
     3961 gcttccgtac gtgatcgagc tcgacgggcg tttctgcggt cagctgacca tcggcaacgt
     4021 cacccacggg gcgttgcgat cggcgtggat cggctactgg gtgccgcggt cggcgactgg
     4081 cggcggggtc gccaccgcag cgttggcgct gggtctcgat cactgcttcg gtccggtcat
     4141 gttgcatcgc gtggaggcca ccgtgcgccc cgagaacgcc gcgagccggg ccgtgctggc
     4201 aaaggtcgga ttccgggaag agggcctgtt gcgccgatat ctcgaggttg acaaggcgtg
     4261 gcgagatcat ctgttgatgg ccatcacggt ggaagaggtt cacggatcgg tggcatcggc
     4321 cctggtccgc tccggacacg ccgggtgggc gtgagctcgg gcgccgttgg ccgcatcgcc
     4381 gacacgccat aggagaaatc cccgccgcca tgaatccggt cgctggcgcg acacgagtga
     4441 cgtgtggtgc ttgtgagagc caaattacag gtgtgtaatt gtgctggtca cttctggccg
     4501 gcccgcgctg gccttcgccg gcctcgtggg gaaaggagca ggtcatcatg ccaagcatcc
     4561 cgcagtcgtt gttgtggatt tcgctcgtgg tgctctggct gttcgttctg gttccgatgc
     4621 tcatcagcaa acgcgacgcc gtgcgacgca ccagtgacgt cgccctggcg acgcgggtgc
     4681 tcaacaacgg tgccggttcc cgtctgctca agaagcggac cggccctgcc gcgggtcatc
     4741 gcagtgaccc gcactggaag gcggaggaag accccgccga agacgagctc gccgacgccc
     4801 aacaggtcca ccgcgacgac gttcacgagg accacgatca ggaagacgac accgaggagg
     4861 tgcagtccgc acctccggtc gtgatgaagg ttgccgtcaa tgagcgcaag gagcccgact
     4921 acctggacgt tgaggtcgtc gaagattccg gggcgctccc agcgggggcc agcgcgacgg
     4981 cggccgagcc cgggtccgac gacatcgacc aggagggcgt cgacgccgag tctgtgagcg
     5041 tcggccagca cggtgaggga gccgatgcag cggcggaccg gggtgatgag tacgaatacg
     5101 tcgaggactc ttcgggtttg gagcccgagg cggaccgcga cgagggcgag gacgaagacg
     5161 cacgggcgtt cgtcgcttcg ggcagctccc gtcggcgccg attcgacacg aagaccgcgg
     5221 ctgcggtgag cgctcggaaa tacgccttcc gcaagcgcgt gctcatggcg atggcggtca
     5281 tcttggtcgg gtcggccacg gccgcattcg aggtgtcgtc gaccgcgtgg tggttctgcg
     5341 ggaccgccac cgcgatgacg atggtctatc tggcctattt gcgtcggcag accaggatcg
     5401 aggagagggt gcgtcgtcgc cggatgcagc ggatggcacg cgcgcggctc ggcgtggaga
     5461 acacctacga ccgcggtcag gacgtggtcc cgtcgcggct gcggcgcccc ggagcggtgg
     5521 tcctggagat cgatgacgag gacccgattt tcgagcatct ggacaacgcg atgccgatgc
     5581 ggaccttcgg ttggccccgg gacctgccgc gcgccgtcgg cgagtaacgc cggcagttcg
     5641 gccgctgggg tcgaggctgg tagcctgcta gcgatcaggg gctatggcgc agttggtagc
     5701 gcgactcgtt cgcatcgagt aggtcagggg ttcgaatccc cttagctcca cccagagaaa
     5761 cgccaactag agcaccgtct gagagcgcag atctcttccc ggacgcgcgt ccgcggcggt
     5821 cgttgcccgg attagcccgg cggtgctcaa cccgtcgccc tatacatctt tcttttagtg
     5881 ctagttcttc gagcccttct acagagcccg attgaccgcc gcggtccgcg cggtcaggta
     5941 tccgcccgta tccgacgaag cgcttcggga ttccgcccgg tctgttcttg acgtcacagc
     6001 cttagtacat ggatgcttgg cgggctctgg taaaccacga gttggccaat atcgtttaca
     6061 taggttcgtt tggtatgtaa gctacggtga ttagtagagt agcggtatga ttcaacgcag
     6121 gcaggtcgct gaaccgatcg gtcgtcaggc ccagccggag gcggttcgct gagtgccaag
     6181 ccgacgctga accaaccaca tcgtttccga ccgaccgagg ggagcgacca ggtgcgagtc
     6241 gcgcagcact tcatgtacag attggcggcc gaaaaatgac cagcaggcgg caggacccat
     6301 cgatagcgcc actccggtcg gcagggctga tagacctcaa tgatctgccg atcggtctga
     6361 ttgacctcga attgagcgcg ccgccaacag gactcgtcgg cgaaacggcc gcgttcctat
     6421 tgcgtcccga gcagggaccg cccgcggccg ttctgaaggt ggcgcgcggc acgctcaagg
     6481 cctcggaact gcggacgcag cggcgtgtgc tcaccgaact cgccatccac caaggactcg
     6541 acgacgaatg gcggggactg ctgccgcgga tcctggcctt cgacgagcgc gctgatgcaa
     6601 ccgtgtcggt cgagtcgtat cggccgggaa cggaccttgc agaagtgctg gcgcgccgcc
     6661 ccaaccgtgt cgaggagctg accactgcgg cgctcagggc catcgcgccg ctgcaccggc
     6721 ggactgctac gtcgatcgtc gtcgacaacg tgtgcttgtt gcggcgatgg gtggtcgaac
     6781 cattggcggg tctgacggag atgtgccgtc ggctggatcc ccgcatggtt ccccatttgg
     6841 aagggcttgg aacgatgctg cggcaggacc ttgttggccg gcgtatgccg gtcagctgga
     6901 cgcacggcgc cttcaccccc gaaaacgttc gggtgggcgg cgtgcggggc ccggtcaccg
     6961 gcatcgtgga ctgggccggg gcgcggccgg gtcagccggc actgatcgac gagtacctga
     7021 tgatcctgac ggcgagttgc caagtggagc aagcggatct gggcaccgtg gtcgctgagc
     7081 ggatacgcgc gggcggtctc tccgagagtg agcgcaatgc gctgggcgcc gcacgggacg
     7141 gatcggacgg ggacgctggc gagcgtgggg cagtcgacga gggcattgct attctcttga
     7201 cctggcttca ccacgccgcc gacctgtggc gtaggtctgc catccacccc aaccaccaca
     7261 cctggtgggc caccaacgtg actccggtga ttgacgccgt cgttgcttcc gacgcattcg
     7321 atgtctcaag cgggcgggcc cgttctggcc gccgcgcggc aagcgtcacg tccatgactg
     7381 cgtcgccgcc ggccgaaggt ggcgcacagg ccatgggaaa tcaggcaaat ggttgatcat
     7441 gcaaaatcca cgacgaacgc tcaccaaata tgcccggcca gaacctggcc gggttggtga
     7501 gccggctgtc gtcgggaccg gcgtagcagc ggctcaccgg ggaacaccaa ttatgttcgg
     7561 cgggcgggcc tccggagtgg tccaggaggt cgtggaggat gatccgagcg ttgccggccg
     7621 gctagcctgt ctcggcgctc ggcttactat ccgcccagcc ctggacatcg gcagccgctt
     7681 ggcgaacgcg ccgtggcctt ggggcctagt cgatttcgct gcccgggccg tgacgcccac
     7741 gccgggcagc gttcgcgcca cggttcagtt tccgcactgc acagcgcgat tggtgcgtgc
     7801 tgccggtgtt ctgcctgccg acggtagcgg gcacgtcgtc ttgtatatgc atggtggcgg
     7861 ctttctgacc tgcggcgtca acacgcatga tcggctcgtc accacgttgt cggagtacgc
     7921 cgatgcgccg gtgctggtga tcaactaccg aatgttgccc aagcattcgc tcggtgaggc
     7981 gatcgacgat tgctatgacg gctactggtg gttgcgtgag cagggttatg cgccggggca
     8041 aatcgtcttg gccggcgatt ccgccggcgg ctacctgtca ctcacgctgg ccgaacgact
     8101 gctcaaagaa ggcgaggagc ccgcggccat cgtctgcatg tcgccgctga tgcagatcac
     8161 caagttcccc aaacaggctc acctcaacat tcgtcacgat gcgatgttcc cggccaaggc
     8221 ttttgacgca ttcgtcgacc tgatcggcaa ggcggcccag cgccacatca tggatgggcg
     8281 ccccgaagaa atctacgagc cgctggacca catcgagcct ggcctgcctc ccacgctgat
     8341 ccatgtgtcg ggttccgagg tcctgctgca cgacgctcgg ctgactgcgc ggaggctggc
     8401 tgcggctgga gtgcccgtcg agctgcgggt atggcctggc caaatgcatg tcttccagat
     8461 cgccgggccg atggtgccag aggccgaccg ctcgctacgc cagataggca actacatccg
     8521 cgaggcaacc gacccatacc cccgccggac ggttcgtacc gccggcgtcc gcacaccggg
     8581 atctcgccgc ggtgtcactg ccgactccgg cccaaaccgg ttgccgccgt ccatgattca
     8641 ccgtcgaact ggagttagct gattatgcgt accctcgtta ccggcgctgc cggattcatc
     8701 ggttccactc ttgtcgaacg cctgctccag gaagggcacc aagtgacagg ggtcgacaat
     8761 ctcagcgccg ccccgcccgt cgccaatctc gcgtcggccc accggcataa gggccctggg
     8821 cgcttcaact tcgtcgccgt cgacgtccgg cacccggagc tggccgacat catcgtgggg
     8881 tccaaccctg acgtggtgtt ccatctcgcc gcccaggtcg acgtgggctg ctccgtgagt
     8941 gatccgcaat tcgacgcccg caacaacgtg ctgggcacca tcaacctgct ggaagcgagt
     9001 cggcgtgccg gagtgacgcg gatcgtctac gccgcctcgg gtggctcccg ctacggtgcg
     9061 ccggcgactt tgcctgccac cgaggaaaca gcggtggacc cactctcccc gggcgcggcg
     9121 gccaaggtcg ccggagagtt ctacatgcgc gcctacgccg gcatgtacgg gctggagccc
     9181 atttgtctgg cactcgccaa cgtctatggg ccacgccagg ttccgcgcgg ggaagccggg
     9241 gttgtcgcgg ttttcagcac tgcgatgatg tccggccgac ccacgaccat ctacggcgat
     9301 ggtagcgcca cccgcgacta cgtctacgtc gacgacgtcg tgaccgcctt cctgtgcgcc
     9361 gcggaagcgc cggacatgac gaccggcacc ttcaacatcg gcaccggggt acaaacgacg
     9421 gtcaacgagc tgcacgatat gatcgcggcc gctgtcggcg tcgcacatcc gcccattcac
     9481 gctgcggccc gcactggcga ggttcgtgtg agcgcattgg atccaaccct ggcgggccag
     9541 gtgctgggct ggaaacccga catcgatctg gccgaaggca tccagcggac cgtcggctgg
     9601 ctgcgcggtc gcctcgatcg ccagccggct gcactcgctc gcgtctagca gcgccgttca
     9661 ccagcgcgag ggcgagccag tcggctaggt gcaaccggcg gtcgcacgta agccgaccac
     9721 tcctcgagtg ttcttgagag ctcgttgaag gcgcgaaggc agaaaggtaa ccacacgacg
     9781 tcaagtaagt gaacagcggc caggccacct ggagaatcag ttgcgtccag tagccatagg
     9841 cgcccatctg caacacgaac ttcagctgaa gtcggatgtc cgacactcgg cggatttcgc
     9901 tatttttagg ttttgatcgt gtctcgttgc tactgtgcca tccatggtgg atgcgcggtt
     9961 acttgctggg ggctcacaca gccagccacg cgacagcgct ggtgtggctg gcagtcaaca
    10021 gatcgagcgg cggcgtagtg ggaggtccgg cgagtccccg acggacatcc tgacgcggtt
    10081 gcccgcggtg gtggtgttgg agcgggtccc ggttcctacg ctcgccatgg cacgggacgg
    10141 catgatcttg tttgccaata cggcgtttgc cgacatggtg ggctacgagc aggacacgct
    10201 ggcggggttg gcgtttccgg agatctttca caccgtgccc gccgaggtcg ccgcgctttc
    10261 cggggtcgag gcgctggcga acctggtggt ggagttgcgg cactgtgagg ggtggacggt
    10321 gcgcgccagg atgagcaagt cgctgatgcg gcgcgatgat cccgtcgttc tggtgacgtt
    10381 tgagaacttg acggagcgac tctgggtgga cgagcactag caaggttgca gtgctcattc
    10441 gctaccgtca cgaattcgcc cgtctcgcgg gttggaaact cacgattgtc ggtcggtgat
    10501 cggtgatacc ggcgatgtgg tgctcgggag tcgacattcc acggatcacc tgacgcgatc
    10561 gcccgcgcgt agcgccttcc gtgttccatc gaggccgcca cgcacctgcg gggccattcg
    10621 cgccgagccc gcaccggcgc caggggcaag tctcggcaag attaccgccg tcgatccctc
    10681 caaaagtgcg tagaagaccg acgcggcgtg cggtggcgac ccgtctgtca ggttgtccgg
    10741 ttgtccacgg cctgctctct agctggcgtt ccccgcttgc gggagcttgg aatcgcaaaa
    10801 aatactgcca gtttgttaaa tcgtttacta cataccaata ggtatgtata ctcctcattc
    10861 aggtgtatcg ccggtatatg cggtcgcccc gaggtgccgc ggcaaggcgg tcgcactcgg
    10921 gtcggtgtgc tttcgatagg aggggcgcat gtcgattgtg accacagacc cggggatccc
    10981 gacgtccgtg cggttgctcg ttgggcccgc ggcgtgagtt cgaccaaagg ggtcaccggg
    11041 cgtctcgccc gcaacgccgc cgtagccaag atctactaga cgacctacga accactagga
    11101 agccatggcg cacatagcct ttcccgacac cgacgtcagc gtcctcgacg ctgacgacct
    11161 cggtgacgtg gtggcggact tcaccgctgc tgcgcaagac cgggctggcc gacctgccat
    11221 cgtgcacaac gggacggtcg tgacctatga agaccttgcg gagcgggtcg gtctcgccgc
    11281 gtcgcgctac cgcgcgtgcc gattcgacgg cgatgggcca tccggtctga tcggcgtgtt
    11341 ggtctcgcac actcccgccg tggttgagca cctgcttggc atactgcaag cccgcgccac
    11401 gtactgcccg atagacgcca ctcttcctgt ggcccgcaag caggcgctcg ccgcggtgct
    11461 cggcctggac cgtctgtttg cgacggcccc ggaccagatc gaacagtcca gcctgcggat
    11521 tgagaccctc gaccaggccg atgtgcccgg ggacaccgac ctgccgcagc cgttgtggca
    11581 ccgcagcgac cccgcctacg tgctgtgcac gtcgggttcg accggagcgc cgaagccggt
    11641 ggtggtctcg cggcatgccg taacctccac ggtgcgcgca ctgcgcgacc tcttcgcgct
    11701 cacccctgag gaccgcgtgc tccaattcgc gtcgctcggc tgggacacct gcttggagga
    11761 gatcttgccg gcgctgaccg ccggcgcgac gttggttttc gacaacgaag cccattccgg
    11821 ctcgatgccg cgtttcgtcc gcatgctggc ggagcgggaa atcaccgtgc tcgacctgcc
    11881 gaccgccttc tggcacgagt tggtgctttt cctggacgag gagcgcgcgg cactgccgga
    11941 cagcgtccgg ctggtggtga tcggcggtga gcgggtcgat ccgacccgac tgcggcagtg
    12001 gcgtgatctc gggaccggtc acgtgaggtt gctcaacacc tacgggtgca cagagacgac
    12061 gatgatcacc catgcggtgc agctggccgg gcccggcacc gagccagaac tcgcggccca
    12121 ggtcgacgcg cccttggggc gtccgctgcc ccatgtgcgc gatcacgtca ccgatgaggg
    12181 cgaactgctg atctcggggc cgtcgatcgc caccggatac ctcgggatgc cggaagtcac
    12241 cgcgaagggg ttcccggtcg ccgattacgg ctccggaccc acccgatggt tccgcaccgg
    12301 agacgtggtc acccgcggcg aacgcgggct ggtgtatccg cgggggcgcg ccgacgagca
    12361 ggtgaaagtc cgtggtgtgc gcgtacatcc ggccgaggtg gaggcccagc tgaacaccca
    12421 cccagcggtc gcgggcgccg tcgtggtcgg cgagcgactg gttgggcaca cgtcgttgac
    12481 ggcgtacgtt gttgcggccg cgacgactac tcccgctgag ctgaggcgtt acctaagtga
    12541 g
//
LOCUS       DASSGQ010000286        17892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1478396,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000286 DASSGQ010000000
VERSION     DASSGQ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 17892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17892
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478396"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..579)
                     /locus_tag="VFI55_12550"
     CDS             complement(16..579)
                     /locus_tag="VFI55_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7074898.1"
                     /translation="MDRPTYHHGDLRAVILTEAARLVAERGADDVSLRELARSAGVSH
                     AAPAHHFTDRRGLFTALATQGFELLTQALVDARGDFADAALAYVRFAIANPGHYQVMF
                     NKSLLDTAEPELAAAEAAAGAELMRGVATLQDPHARADPAAAQLAAWSLVHGFSTLWL
                     NDAVNAEVRQLDPMDTVRRIAMMLFEG"
     gene            676..1131
                     /locus_tag="VFI55_12555"
     CDS             676..1131
                     /locus_tag="VFI55_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074899.1"
                     /translation="MAPLITLLLSTIAARSIGWLGWFDAAYVNSWNAAIAVGLAAMFV
                     LTGISHFAPPLRGDLIAIVPPRLPAPGLLVTITGVLELLGAAGLLVPNTRFAAAVCLL
                     ALMLAMFPANVYASRMPHPPESMTTRLPLRTAFEVVFLAAAIAVAVGSG"
     gene            complement(1060..3231)
                     /locus_tag="VFI55_12560"
     CDS             complement(1060..3231)
                     /locus_tag="VFI55_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016706123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET7074900.1"
                     /translation="MTDASLEARVPPLPPTAKRVETRREHHGDVFIDPYEWLRDKADP
                     EVIAYLEAENDYVDQTTAHLEPLRQQIFDEIKSRTKETDLSVPTRQGDWWYYARTFEG
                     KQYRVQCRCPIVDPGDWDPPTLDENTEIPGEQVLLDSNVEAEGHEFFALGAAMVSLDG
                     NLLAYSVDTVGDERYTLRFKDLRNDEQYPDEIADIAAGVTWAADNRTVYYLTLDAAHR
                     PDKVWRYRLASGDPSELVYHEADERFWLGMGLTRSEKYVVIASGSSITSELRYADSAD
                     PHAGFTVVLPRREGVEYSVEHAVVGGEDRFLILHNDDAVNFMLAEAPVGDPTQQRTLI
                     PHRDDVRLDGVDAFAGHLVVSYRREALPRIQLWPIDSDSGYGAPAEISFDSELMWCGL
                     GGNPTWDSPKLRIRAGSLVTPAQVYDIDLATGERTLLREQPVLGGYRPADYVERRVWA
                     PAADGTRIPVSIVYRDDIEFPAPALLYGYGAYEICTDPGFSIARLSLLDRGMVFAIAH
                     VRGGGEMGRLWYEHGKLLEKKNTFTDFVSVARHLVDSGLTTPQRLVALGGSAGGLLMG
                     AVANLAPDCFAGILAQVPFVDPLTTILDPSLPLTVTEWDEWGNPLDDGDVYSYMKSYS
                     PYENVEAKRYPAILAMTSLNDTRVYYVEPAKWVAALRHANSNGTAFLLKTQMNAGHGG
                     VSGRYRGWEETAFQYAWLLAAADGDRYGGGQEDDLEGGPQG"
     gene            complement(3228..4121)
                     /locus_tag="VFI55_12565"
     CDS             complement(3228..4121)
                     /locus_tag="VFI55_12565"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET7074901.1"
                     /translation="MRPALSDYEHLASGKVREIYRVDNEHLLLVATDRISAYDFVLDS
                     TIPDKGRILTAMSVFFFGLVDAANHLAGPPDDPRIPAEVLGRALVVRQLQMLPVECVA
                     RGYLTGSGLLDYQATGKVCGITLPPGLVEASRFATPLFTPATKAALGDHDENISFAQV
                     IEMVGGVIANQLRDRTLQIYVQAADHALTRGIIIADTKFEFGTDPDGNLLLADEIFTP
                     DSSRYWPAEDYRVGEVQTSFDKQFVRNWLTSPESGWDRHGSQPPPPLPDDIIEATRDR
                     YINAYERISGLSFDDWIGSGA"
     gene            4186..4806
                     /locus_tag="VFI55_12570"
     CDS             4186..4806
                     /locus_tag="VFI55_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074902.1"
                     /translation="MRSRFLPYATTPGRLVAQLFSDFAIFIWTAIWLLVGVAVYDAIS
                     EIADAGRQVETGANGIAGNLASAGQGAQHIPLVGDAVSTPLTSASAAARDIAGAGHSL
                     DATASWLAVLLALAVVAVPILIVVVPWLFLRLRFFRRKWTVTGLAATPAGEQVLALRA
                     LTNRPPRKLAAISADPVGAWRREDPLAMRALAALELRAAGITVRAG"
     gene            complement(4771..5817)
                     /locus_tag="VFI55_12575"
     CDS             complement(4771..5817)
                     /locus_tag="VFI55_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HET7074903.1"
                     /translation="MTIRFADVGPSPDVFAAVMATGVLSIVARDHRYRWLGDTLGVLA
                     TLGLLVLVVMVIVTTGLAFSGRGPVRWDLTDPDVTVRLFTFVAACAVVDTRLSSNVWV
                     LRVLGAVGLSAWLVLAVLSVRNMRAHRWAVLRDRAHGAWELASVGTSALAIMMAALAQ
                     QTGHRWWLAIALPVWVAAICIYGLMTWLILWRAATQRHHPALAGAAFEPDSWILMGGL
                     AIATLAGDKIHALAPAWLAGPVLVVTVVTWVAATVWFPVLIYFGLRRISRGPDLLHFA
                     GVWWALVFPLGMYSAATYTMAAELRQPSLVTASLVFFWDALAAWLIVVVAGLLRLSRA
                     LSPSARTNRDARCP"
     gene            complement(5814..7061)
                     /locus_tag="VFI55_12580"
     CDS             complement(5814..7061)
                     /locus_tag="VFI55_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7074904.1"
                     /translation="MTLAGALSGIDLTDLDNFANGFPHELFAVHRREAPVYWHEPTDN
                     TPDGEGFWSVAVYAETLAVLKDPVTFSSVTGGRRPFGGTLLQDLAIAGQVLNMMDDPW
                     HAQIRRLVSSGLTPRMIRRVEDDLRARTRRLLDEVEPGEPFDFLVDIAAELPMQMICI
                     LLGVPESERHWLFEAIEPQFDFGGSRRAALSQLSVEDSGSRMYAYGQELIASKRAEPT
                     DDMLSVVANALVDDADAAALSDLELYLFFSLLFSAGAETTRNGIAGGLLALAEHPEQL
                     HALRLDFEMLPTAIEEMVRWTSPSPSKRRTATRDVTLGVASIEAAQKVQIWEGSANRD
                     ASVFDRADEFDIARKPNPHLGFGQGVHYCLGANLARLELRVLFEELLSRFSTVQVVQP
                     VEWTRSNRHTGIRHLVVELAEEQ"
     gene            complement(7058..8476)
                     /gene="purB"
                     /locus_tag="VFI55_12585"
     CDS             complement(7058..8476)
                     /gene="purB"
                     /locus_tag="VFI55_12585"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET7074905.1"
                     /translation="MSIPNVLAARYASPEMVAIWSPEAKVVAERRLWLAVLRAQAELG
                     VPVPGDAIADYERVLDDVDLASIAARERVLRHDVKARIEEFNALAGHEHVHKGMTSRD
                     LTENVEQLQIRRSLELVYSHGVAVAARLAERAVTYRDLVMAGRSHNVAAQATTLGKRF
                     ASAAQETLIALTRLRELIDRYPLRGIKGPMGTAQDMLDLLGGDAAKLAQLERRIAEFL
                     DFTTVLTSVGQVYPRSLDHDVVSALVQFGAGPSSMAHTIRLMAGHELVTEGFAPGQVG
                     SSAMPHKMNTRSCERVNGLQVVLRGYASMAAELAGAQWNEGDVFCSVVRRVALPDSFF
                     AIDGQIETFLTVLDEFGAYPAVIQRELDRYLPFLATTKVLIAAVRAGMGREAAHHVIR
                     EHAVATALAMREKGAEPDLLDRLAADERLPLDRAALDVALADKKAFIGAAGDQVDDVV
                     AMVDTLVSRYPEAAKYTPGSIL"
     gene            complement(8671..9306)
                     /locus_tag="VFI55_12590"
     CDS             complement(8671..9306)
                     /locus_tag="VFI55_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET7074906.1"
                     /translation="MAPWLLVTAAVTPKGERRRYALVSAAAELLAEGGFEAVRHRAVA
                     RRAGLPLASTTYYFSSLDDLIARAVEHIGMIEVAQLRARVTALSRRRRGPETTAEVLV
                     DLLVGDVSGPGLAEQLISRYERHIACTRLPELRETMRRSLRQRADAVAEAIERSGRSV
                     HIELVCTLICAVDGSVVSALVEGRDPRAGALATVVDLVEVLAPIDTRPVRI"
     gene            9350..10279
                     /locus_tag="VFI55_12595"
     CDS             9350..10279
                     /locus_tag="VFI55_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET7074907.1"
                     /translation="MMARMPDLSRRALLGLGAGAAVGAVGGYALEVLVQPRTSHAAPV
                     ATPVSNAGSRVPLAPAPSAPLEPVPSPSPAPTMVTGSFVSAARGGIPTNWAIARPPGQ
                     TKALRPVIALHGKGSDAATVMAGGVEQGLAQAVDAGLPPFAVVAVDGGGSYWHKRASG
                     EDAGAMVLGELIPMLDNQGLDTSRVAFLGWSMGGYGALLLGGRLGPARTAAICAVSPA
                     LWLSSGAAAPGAFDGPDDFSANSVFGMPALASIPIRVDCGDSDPFYDATRQFIAQLPN
                     PPAGGFSPGGHNGEFWSSQLPAELTWMAPLLTA"
     gene            10310..11893
                     /locus_tag="VFI55_12600"
     CDS             10310..11893
                     /locus_tag="VFI55_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HET7074908.1"
                     /translation="MTTPFGWSFPYAWPRKPILAGNVVCTSQPLAAQAGLRMLADGGN
                     AVDAAIATAITLTIVEPVSNGIGSDAFAIVWNGKRLHGLNASGRSPAGWTPEYFGDNA
                     VPALGWNSVTVPGAVSAWTELHAKFGKLPFEKLFEPAISYGRNGFLVSPTVADQWAAQ
                     VPVFKTQPGFAEAFLPAGRAPKPGELFRFPDHAATLELIAETRGEAFYRGELAVKLEE
                     HASSNGGVLRAGDLAAHHADWVDTIHGNYRGYTVHEMPPNGQGIVALIALGILEHFDM
                     STLPADSADSVHLQIEALKLAFADAHAHVADIDHMALAPERLLDRDYLQQRAALIDPK
                     RAKPASAGIPSGGTVYLTAADATGVMVSMIQSNYMGFGSGVVVPGTGISLQNRGAHFA
                     TAQGHPNRVGPNKRPYHTIIPGFVTKDGVPVMSFGVMGGTMQPQGHVQVLARIADHGQ
                     NPQAACDGPRFRWVQGMQVSCEAGFPRSTLDELRQRGHDLIAVDDYNQFGSCQAIWRL
                     DDGYFAASDPRRDGQAAAF"
     gene            11979..12710
                     /locus_tag="VFI55_12605"
     CDS             11979..12710
                     /locus_tag="VFI55_12605"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7074909.1"
                     /translation="MARTVNVAEHAARRDEILDAAQRLILSAGYERLTVQDILNDLQI
                     SKGAFYHYFDSKPTVIEALTRRLVSDSERALAPIAENPEMGAVTKLHAFFGEIIRWKS
                     ERQNLFVAMLPAWYAPDNLAFRLLVDRESAQRLGPLLSTIVGQGVDEGQFLTAYPDQA
                     GAIIFAIIQALQDAMAQKLLEVQQRSPSSPIVNDLVNTHGAHMEAIERYLGVPMGTLN
                     RVDARAVKSWITALHRAYTAAHGKR"
     gene            12718..13923
                     /locus_tag="VFI55_12610"
     CDS             12718..13923
                     /locus_tag="VFI55_12610"
                     /inference="COORDINATES: protein motif:HMM:NF004834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7074910.1"
                     /translation="MEIRQTTCLVAGGGPAGMMLAYLLARSGVQTTLVEKHGDFLRDF
                     RGDTVHPSTTELMHELGLLEDFLQQVPHEAMEQLGIGVKGQTYDLIDFRHLPTACKYV
                     VFMPQWDFLNFLADKAKRYPSFRLEMSTEVTGLLWQGDRVAGITAQTAGDPVEIRADL
                     TVACDGRWSVIREHSGLPVTEFPMPIDVLWFRLPRIGSAPETLGYLGGGQIVIAINRG
                     EYWQCGTIIDKDGFDRVRAEGLQAFRSSVAAAAPFLTASLAALTDWDQVKLLSVRANR
                     LRRWWLPGLLCIGDAAHAMSPVGGIGVNYAVADAVAAANALAEPLRNGRVSADDLRAV
                     QKRRLIPTRIAQRLQGAQTANLARMSKTEPPLWMLRLLSHCAPAKRLLGRTVGLGFRR
                     EHIRTVERS"
     gene            complement(13920..15188)
                     /gene="purD"
                     /locus_tag="VFI55_12615"
     CDS             complement(13920..15188)
                     /gene="purD"
                     /locus_tag="VFI55_12615"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET7074911.1"
                     /translation="MRVLVIGSGAREHALLLALRRDPQVTGLAIAPGNAGTARVAEQH
                     DVDITSSEDVVALAREVRADMVVIGPEVPLVLGVADAVREAGIVCFGPSKDAARIEGS
                     KAFAKEVMAAAGVRTATSEIVDSPARLDAALDRFGPRAGDPAWVVKDDHLAAGKGVVV
                     TPDRDAARAHAAALLEAGRPVLLESYLDGPEVSLFCVVDGRTVVPLLAAQDFKRVGDG
                     DTGPNTGGMGAYAPLPWLPDGVSGELVSQIVEPVAAEMVQRGSPFSGLLYVGLAMTKG
                     GPAVVEFNCRFGDPETQAVLALLDSPLGQLLYAAATGQLTDFGEVQWRDGAAVAVVVA
                     AENYPGRPRVGDVIVGSEAEGVLHAGTARRDDGAIVSSGGRVLSVVGTGADLAAARTR
                     AYEILGSIRLPGSHFRSDIGLEAAEGKISV"
     gene            complement(15253..15693)
                     /locus_tag="VFI55_12620"
     CDS             complement(15253..15693)
                     /locus_tag="VFI55_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016706120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7074912.1"
                     /translation="MMDELRRKGLEKMNEVYGWEMPNIEGDAYFDLTVDHLFGSIWTR
                     SGLSMRDKRIMTLTAVTAVGNRDLAEIQINAALLNGELSETELKEMAVFLTHYLGFPL
                     GSALNGAVDTVVAKRKKAAAKGADEDKKANVDAALKMHAGDSSG"
     gene            complement(15690..16571)
                     /locus_tag="VFI55_12625"
     CDS             complement(15690..16571)
                     /locus_tag="VFI55_12625"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7074913.1"
                     /translation="MSKNTENIPRLGYIGLGNQGAPMAKRLLDWPGGLTVFDVRAEAM
                     APFVDGGAVAATTLSDVAEADIISVTVFDDAQVRAVVSGDGGLASRAKPGTIIAIHST
                     IDHTTAAQLARGLKPRGIHIVDAPVSGGAGAAAEGQLATMVGADDESFERLREPFGRW
                     ASLVIHAGEPGAGTRMKLARNMLTFVSYAAAAEAEKLAETSGLSLRQLAKVVRHSDGL
                     TGGAAAIMFRQTTAPFAADDPLRPMLEHTRELGEKDLSLALALGDAVSVDLPLARLAF
                     ERLAIGLGVPHTEKETS"
     gene            complement(16661..>17892)
                     /locus_tag="VFI55_12630"
     CDS             complement(16661..>17892)
                     /locus_tag="VFI55_12630"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HET7074914.1"
                     /translation="MREHVEELRELTIAEVGAPRMLTAAAQLEGPVGDLGFAADTAES
                     YTWNSDLGEAAPMGIPTRRTIAREAVGVVGAITPWNFPHQINLAKLGPALAAGNTVVL
                     KPAPDTPWCAAVLGEIIAEHTDFPPGVVNIVTSCDHALGAFLAKDPRVDMISFTGSTA
                     TGRSVMSDAAATIKKVFLELGGKSAFVVLDDADLGAACSVSAFTAAVHAGQGCAITTR
                     LVVPRARYDEAVAIAAATMSSIKPGDPNEPGTVCGPLISARQRDRVQGYLDLAIAEGG
                     TFACGGGRPANKEAGFFIEPTVIAGLSNDARSAREEIFGPVLTVIAHDGDDDAVRIAN
                     DSPYGLSGTVFSADSERAARIASRLRVGTVDVNGGVWYSADAPFGGYKQSGNGREMGL
                     LGFEEYLEAKLIATVAN"
BASE COUNT         3072 a         6093 c         5744 g         2983 t
ORIGIN      
        1 ggcgaatagc acgcactaac cttcgaacag catcatcgcg atccgcctca ccgtatccat
       61 cggatcaagt tgccgcactt cagcgttgac cgcatcgttg agccacagcg tcgaaaatcc
      121 gtgcaccaat gaccacgctg cgagctgcgc ggccgcggga tcggcgcgcg cgtggggatc
      181 ctgcagcgtc gccacgccac gcatgagctc ggcgccggcg gcagcttcgg cggcggccag
      241 ttcgggttcg gcggtgtcga gcagcgactt gttgaacatc acctggtagt ggccggggtt
      301 cgcgatggcg aaccgcacgt aggcgagcgc cgcgtcggcg aagtcgccgc gcgcatcgac
      361 gagagcctgg gtgagcagct cgaacccctg cgtcgccagt gccgtgaaca acccgcggcg
      421 atcggtgaag tggtgtgccg gcgcagcgtg cgacacgcct gcgctgcgcg ccagctcgcg
      481 cagtgacaca tcgtcggcgc cacgctcggc gaccaggcgc gcggcctcgg tcaggatcac
      541 cgcgcgcaga tccccatggt gataggtcgg acggtccacg gcaacagcat accgggcaga
      601 acttgacact gcctagatgg cggtttacct tcgatcctga cgaacttgtc agtgcctaga
      661 ttggggtatg ttgagatggc gccgttgatc accctgctgt tgagcaccat cgccgcccgg
      721 agcatcggct ggcttggttg gtttgatgcg gcctacgtca acagctggaa cgcggcgatc
      781 gccgtcgggc tggcggcgat gtttgtgctg accggcattt cgcacttcgc gccacccctg
      841 cgcggggacc tcatcgcgat cgtgccgccc cgactgcccg ctcccggtct tctggtcacc
      901 atcaccggcg tgctagagct gctcggtgcc gcaggcctgc tggtgcctaa cacccggttc
      961 gcggcggcgg tgtgcctgct ggccctgatg ctggccatgt tcccggccaa cgtctacgcc
     1021 tcccggatgc cgcatccgcc cgaatcgatg acgacgcggc tacccctgcg gaccgccttc
     1081 gaggtcgtct tcctggccgc cgccatagcg gtcgccgtcg gcagcggcta gcagccaggc
     1141 gtattggaaa gccgtctcct cccaccccct atagcgacca ctgacgccgc cgtgtcccgc
     1201 gttcatctgg gttttcagca gaaacgcagt gccgttgctg ttggcatgcc gcaacgcggc
     1261 aacccatttg gccggttcga cgtaatagac ccgggtgtcg ttcagggacg tcatcgccaa
     1321 gatagccggg taccgcttcg cctcgacatt ctcgtagggc gaatacgact tcatgtagga
     1381 gtaaacatcg ccgtcgtcca atgggtttcc ccattcgtcc cattcggtga ccgtcagcgg
     1441 caacgacgga tcgaggatcg tcgtcagtgg gtcgacaaac gggacctgtg cgaggattcc
     1501 ggcaaaacaa tccggcgcca ggttggccac cgcacccatc agcagaccgc ccgcgctgcc
     1561 gcccagtgcc accaaccgct gcggagtggt caggcccgag tccaccaaat gccttgccac
     1621 cgagacaaag tcggtgaagg tgttcttctt ctccaacagc ttgccgtgct cgtaccatag
     1681 ccggcccatc tcaccgccgc cccggacatg ggcgatggcg aacaccatgc cgcggtccaa
     1741 cagcgacaac cgtgcgatgg aaaagcctgg atcggtgcag atctcgtagg cgccgtaacc
     1801 gtaaagcagt gcgggcgcgg ggaactcgat atcgtcgcgg tacacgatcg acacggggat
     1861 ccgggtgccg tcggctgccg gcgcccagac gcggcgttcc acgtagtcag ccggccggta
     1921 gccgccgagg acgggttgct cgcgcagcag ggtgcgctca ccggtggcaa ggtcgatgtc
     1981 gtagacctgt gccggagtga ccagagaccc cgccctgatc cgcagtttgg gtgaatccca
     2041 ggtggggttg ccgcccaggc cgcaccacat cagctcggag tcgaatgaaa tctccgcggg
     2101 tgcaccgtag ccactgtcgg aatcgatggg ccacagctga atccgcggca gcgcctcgcg
     2161 ccggtagctg accaccagat ggccggcaaa cgcatcgacc ccgtcgagcc ggacatcgtc
     2221 gcggtgcgga atcagggtgc gctgctgcgt cgggtcgccg accggggcct cggcgagcat
     2281 gaaattcacc gcgtcgtcgt tgtgcagaat caagaaccgg tcttcaccac ccaccaccgc
     2341 atgctcgacc gagtactcga cgccttcccg ccgcggcaac acgacagtga accctgcgtg
     2401 tggatcagcg gagtccgcgt agcggagctc tgaggtgatg gacgaccccg aggcgatgac
     2461 aacatacttc tcgcttcggg tcaggcccat gccgagccaa aaccgttcgt cggcttcgtg
     2521 atacaccagc tcggacggat cgccggaggc gagccgatac cgccagacct tgtcgggacg
     2581 atgggccgcg tccagggtca ggtagtacac ggtgcggttg tcggccgccc aggtcactcc
     2641 ggccgcgatg tcggctatct cgtcgggata ctgctcgtcg ttgcgtaagt ccttgaaccg
     2701 caaggtataa cgttcatcgc ctactgtgtc gacggagtag gccagcaggt tcccgtccag
     2761 gctgaccatg gcggcaccga gcgcgaagaa ttcgtggcct tcggcttcca cgttcgagtc
     2821 aagcaacacc tgctcgccgg gaatttcggt gttctcgtcg agggtcggcg gatcccagtc
     2881 accgggatcg acgatcgggc aacggcactg gacccggtac tgctttcctt cgaaggtacg
     2941 ggcgtaatac caccagtcac cctgtcgggt cggcaccgac agatcggtct ccttggtgcg
     3001 cgacttgatc tcatcgaaaa tctgctgccg caacggttcc aggtgggcgg ttgtctgatc
     3061 gacgtagtcg ttttccgcct ccagataggc gatgacttca ggatcggcct tatcgcgcag
     3121 ccattcgtac gggtcgataa agacgtcccc gtggtgctcg cgccgggtct cgacacgctt
     3181 ggccgtgggc ggtaagggcg gtacgcgcgc ctcgagagaa gcgtcggtca tgcgcccgag
     3241 ccgatccagt catcgaaact caaaccggaa atccgttcgt aggcattgat ataacggtcg
     3301 cgagtggcct cgatgatgtc gtcgggaagc ggcggtggag gctgtgagcc gtggcggtcc
     3361 cagccggact cggggctggt gagccaatta cgaacgaact gcttgtcgaa actggtctgg
     3421 acctcgccta cgcggtaatc ctctgccggc cagtaccggg aggagtccgg cgtgaagatt
     3481 tcatctgcca gcagcaggtt gccgtccggg tcggtgccga attcgaactt ggtgtcggcg
     3541 atgatgattc cccgcgtcag ggcgtgatcg gcggcctgca cgtagatctg cagagtacgg
     3601 tcgcgcagct gattggcgat caccccgcca accatctcga tcacctgggc gaacgagatg
     3661 ttctcgtcgt ggtcccccag tgccgctttg gtcgcgggag tgaacagcgg cgtggcgaac
     3721 ctgctggctt cgaccagacc tggcggcagc gtaatgccgc agaccttgcc ggttgcctgg
     3781 tagtcgagca gccccgagcc ggtcaagtag ccgcgcgcca cacattccac cggaagcatc
     3841 tgcagctgcc gcaccaccag cgcgcggccc agcacctcgg cggggatccg cgggtcatcc
     3901 ggcggccccg cgaggtggtt ggccgcgtcg acgaggccga agaagaacac gctcatcgcg
     3961 gtcaggatgc gacccttatc ggggatcgtg ctgtccagga caaagtcgta ggcagagatc
     4021 cggtcggtcg ccaccagcag caagtgctcg ttatcgacgc ggtagatctc gcggaccttg
     4081 ccgctggcca ggtgctcata gtcggacagt gcagggcgca tcgggccagc ctatcggggg
     4141 ccgtaacggc aaccctgggc tgggagctgt gctggtattt aaagtatgag atcgcggttc
     4201 ttgccctacg ccaccacacc cggccggctg gtcgcccaac ttttcagcga tttcgccatc
     4261 ttcatatgga cggcgatatg gctgcttgtc ggcgtggccg tgtacgacgc catctcggag
     4321 atcgccgacg ccggccggca agtcgaaacc ggcgcgaacg gcatcgcggg caacctggcc
     4381 tcggctgggc agggcgcgca gcacattccg ctggtgggcg atgcggtgag cactcccctc
     4441 acctcggcca gcgcggccgc ccgcgacatc gcgggcgccg gccacagcct ggacgccacg
     4501 gcgagttggc tcgcagtact gctggcgctc gcggtcgtcg cggtgccgat cctcatcgtg
     4561 gtcgtgccgt ggctcttcct gcggctccga ttcttccggc gcaagtggac cgtcaccggg
     4621 ctggccgcga ccccggcggg cgaacaggtg ctggccctgc gggcattgac gaaccggccg
     4681 ccccgcaaac tggccgccat cagcgcagat ccggtcggtg cctggcgccg cgaggatccc
     4741 ctcgccatgc gggcgctggc cgcgctggaa ctacgggcag cgggcatcac ggttcgtgcg
     4801 ggctgacggg ctcagggcgc gcgacagccg cagcaacccg gcgacgacca caatcagcca
     4861 cgccgccaac gcgtcccaga agaacaccag cgaagcggtg accagcgatg gctgacggag
     4921 ttcggccgcc attgtgtagg tggcggccga atacatgccc agcgggaaca ccaacgccca
     4981 ccacacaccc gcgaagtgca gcagatcggg cccgcggctg atccggcgca agccgaagta
     5041 gatcagcacc gggaaccaca cagtggccgc cacccaggtc acgacggtaa cgaccaacac
     5101 cggccctgcc agccaggcgg gagccagggc gtggatctta tcgccggcca gcgtcgcgat
     5161 ggccaaaccg cccatcagta tccagctgtc gggctcgaac gctgcacccg ccaaggcggg
     5221 atgatggcgc tgggtggccg cacgccagag aatcagccag gtcattaggc cgtaaatgca
     5281 gatcgccgcc acccagacgg gcagggcaat cgccagccac cagcgatgcc cggtttgctg
     5341 ggccagcgca gccatcatga ttgccaacgc ggacgttcct acgctggcca gctcccatgc
     5401 gccatgggca cggtcccgca gcacggccca tcggtgcgcc cgcatgttcc gcacgctgag
     5461 cacggccagc accagccagg ctgacaaccc aaccgccccg agcacccgga gcacccacac
     5521 attggacgac aggcgcgtgt cgaccacggc gcacgcggcc acgaaggtga acagccgcac
     5581 ggtgacgtcg ggatccgtca gatcccagcg caccggtccg cgcccactga aggctaggcc
     5641 ggtcgtcacg atcaccatca caaccaggac cagcagaccg agagtggcta ggacccccag
     5701 ggtgtcgccg agccaccggt accggtgatc gcgggcgaca atcgacaaga ctccggtcgc
     5761 catcaccgcg gcgaacacat cgggtgacgg cccgacatcg gcgaaccgaa tcgtcactgt
     5821 tcctcggcta gctccacgac cagatgccgg atgccggtgt gccgattgct gcgcgtccat
     5881 tcgacgggct gcaccacctg cacggtggaa aagcgcgaca gcagctcctc gaacagcaca
     5941 cgcagttcca gcctcgccag attggcgccc aggcaatagt gcacgccctg gccaaagccc
     6001 aagtgcggat tgggtttccg ggcaatatcg aactcgtcgg cgcggtcgaa cacgctggcg
     6061 tcacggttgg ccgagccctc ccagatctgc accttctgcg ccgcctcgat cgaagctacg
     6121 ccgagcgtga cgtcgcgggt ggcggtgcgc cgcttggacg gcgacggcga cgtccaccgg
     6181 accatctcct cgatcgccgt cggcagcatc tcgaaatcca ggcgaagcgc gtgcaattgc
     6241 tcggggtgtt cggccagcgc gagtaggcca cccgcgatcc cgttgcgtgt cgtctcggcg
     6301 ccggcactga acagcaggct gaagaacagg tacagctcga ggtcggacag ggccgccgca
     6361 tccgcatcgt cgaccaacgc attcgccacc accgagagca tgtcgtcggt cggctcggcg
     6421 cgcttggacg cgatcagctc ctggccgtag gcgtacatcc gcgacccgga gtcttcgacg
     6481 gacagctgcg agagggccgc cctgcgtgag ccaccgaagt cgaactgcgg ctcgatggcc
     6541 tcgaacagcc agtgtcgttc cgattcaggc actcccagca ggatgcagat catctgcatc
     6601 ggcagttcgg ccgcgatgtc aaccaggaag tcgaacggtt cgcccggctc cacctcgtcg
     6661 agcagacgac gcgtgcgggc gcgcagatcg tcttcgaccc ggcggatcat ccgcggcgtc
     6721 agcccggagc tgacaagtcg gcggatctgt gcgtgccacg ggtcatccat catgttgagg
     6781 acttggcccg cgatggccag gtcctgcagg agggtgccgc caaaaggtcg ccgcccgccg
     6841 gtgaccgacg agaaggtcac cggatccttc agcaccgcaa gcgtttccgc gtagacggcg
     6901 acggaccaga agccctcgcc gtcgggcgtg ttgtcggtcg gctcgtgcca gtacaccggc
     6961 gcctctcggc gatgaacggc gaacaactcg tgcgggaatc cgttggcgaa gttgtccaga
     7021 tcggtgaggt cgatcccgga gagcgcgccc gcaagagtca caggatcgag cccggggtgt
     7081 atttggccgc ctccgggtag cggctcacca acgtatccac catggccacg acgtcgtcca
     7141 cttggtcacc ggcagcaccg atgaacgcct tcttgtcggc cagcgccacg tccagagcgg
     7201 ctcggtccag cggcagccgc tcgtcggcgg ccagccggtc cagcaggtcg ggctcggcgc
     7261 ccttctcccg catggccagc gccgtcgcca ccgcgtgttc gcggatcacg tgatgcgcgg
     7321 cctcgcgacc cattccggcg cgcaccgcgg cgatcaacac cttggtggtg gccaggaacg
     7381 gcagatagcg gtccagctcg cgctggatca ccgccgggta ggcgccgaac tcgtcgagca
     7441 ccgtcaggaa cgtctcgatc tgcccatcga tggcaaaaaa gctgtccggc aatgcaactc
     7501 ggcgcaccac tgagcagaac acgtcgccct cgttccactg cgccccagcc agttcggcgg
     7561 ccatggacgc gtagccgcgc agcaccacct gcaacccgtt gacccgctcg caactgcggg
     7621 tgttcatctt gtggggcatc gccgacgagc ccacctggcc gggtgcgaac ccctcggtga
     7681 cgagttcgtg cccggccatc agccggatgg tgtgcgccat cgacgagggt ccggcaccga
     7741 actgcaccag cgcggagacc acgtcgtgat cgagcgagcg cgggtacacc tgcccgacac
     7801 tggtcaaaac cgttgtgaag tccaagaatt cagcgatgcg gcgttctaat tgggccagct
     7861 tggccgcatc gccgccgagc aggtcgagca tgtcctgcgc ggtgcccatc ggccccttga
     7921 tgccgcgcag cgggtagcgg tcgatcaatt cgcgcagcct ggtcaacgcg atcagcgttt
     7981 cttgcgcggc ggacgcgaac cgcttgccca aagtcgttgc ctgcgcggca acattgtggc
     8041 tgcgcccggc catcaccaga tcgcggtagg ttaccgcacg ctcggcaagc cgtgccgcga
     8101 ccgcgacccc gtgcgagtaa accaattcga gcgaccgccg gatctgcagt tgctccacat
     8161 tctcggtcag gtcgcggctg gtcatccctt tgtgcacatg ctcgtggccg gccagcgcat
     8221 tgaattcctc gatgcgggct ttgacgtcgt ggcgcagcac ccgttcgcgg gcagcgatcg
     8281 acgccaagtc cacgtcgtcg agcaccctct cgtagtcggc gatggcgtcc ccgggaaccg
     8341 gcacacccag ttccgcttgt gcccgcagca cggccagcca tagtcgccgc tccgcgacga
     8401 ccttggcctc cggcgaccag atcgcgacca tctcggggct ggcgtaccgg gccgccagca
     8461 cattcggaat gctcacaaac acacagctta cggtcggccc gttccgctga ccggcttccg
     8521 gtcgctgttg ccacatcggt ctctgcaccg gcccgcgcac aaattgccca cctcaaagag
     8581 cacccatgtc agttgaagcc aacgtgacga gatcccccga ggcgaacgcc gccttcgctg
     8641 ccttccgatc tggctcgccg gtagcggctc tcagatccgc acgggtcgcg tgtcgatcgg
     8701 agcaagcact tccacgaggt ctaccaccgt cgccagcgcg ccggcgcggg gatcgcgccc
     8761 ctcgaccagg gcggagacca ccgaaccgtc gaccgcgcag atcagcgtgc acaccaattc
     8821 gatatgcacg gatcggccgg agcgctcgat ggcctctgcc acggcgtcgg cacgttgtcg
     8881 caggctgcgg cgcatcgtct cacgcaactc gggcagacga gtgcaggcga tatgccgctc
     8941 gtatcgcgat atcagctgct cggccagtcc cgggccggac acgtccccca caagcaggtc
     9001 aaccagcacc tcggcggtgg tctcgggtcc gcggcgccgc cgggacaagg cggtcacccg
     9061 cgcccgcagc tgcgccacct cgatcatgcc gatgtgttcg acggcgcgcg cgatcaaatc
     9121 gtccagggaa gagaagtaat acgtggtcga agccaacggc agaccagccc gccgcgccac
     9181 cgcccggtgc cgcaccgctt cgaacccacc ctcggcaagc agctcggcag cggcgctcac
     9241 gagcgcatac cgccgacgtt ctcccttggg agtaactgct gcagtcacga gtagccatgg
     9301 tgccagtcag agtggtacag cattgccatt tcggccaaac ggccatggca tgatggcccg
     9361 catgcccgac ttgagccgcc gcgccctgct cggtcttggc gccggcgccg ccgtcggtgc
     9421 cgtgggggga tacgccctcg aggtcctggt ccagccccga acgtcgcacg ccgcgccggt
     9481 ggccactccc gtttccaacg cgggcagtcg ggtgccgctg gcgccggcgc cgagtgcacc
     9541 tctcgagccg gtgccctccc cgtcgccggc accgacgatg gttacgggct ctttcgtgtc
     9601 ggcggcgcgc ggtggcatac cgacaaactg ggcaatcgcg cgtccccctg ggcagaccaa
     9661 ggcgctgcga cccgtgatcg ccctgcacgg taagggcagt gacgccgcca ccgtgatggc
     9721 cgggggcgtc gagcagggtc tggcccaggc cgtcgacgcc gggctgccgc cgttcgccgt
     9781 ggtcgcggtc gacggcggcg gcagttattg gcacaagcgg gcttccggcg aggacgccgg
     9841 agccatggtg ctgggcgagc tgatcccgat gctggacaac cagggcctgg acacctcacg
     9901 ggtggccttc ttgggctggt cgatgggcgg ctacggcgcg ttgctgctcg gcggacggtt
     9961 gggacccgcg cgcaccgccg caatctgcgc ggtgagcccg gcattgtggc tgtcctccgg
    10021 tgcggcggca cctggtgcct tcgacgggcc cgacgacttt tcggcgaact cggtgttcgg
    10081 gatgccggcg ctggcatcga tcccgatccg ggtggactgc ggcgacagcg acccgttcta
    10141 tgacgcgacg aggcagttca tcgcacagct gcccaacccg cccgccggcg gcttttcgcc
    10201 cggcggtcac aacggagagt tctggagctc gcaactgccc gccgagctga cctggatggc
    10261 gccactgctc acggcgtgac ggccggcggc cagggttacg ctcgcgcacg tgacaacacc
    10321 gttcggttgg agcttcccct acgcttggcc gcgaaagccg atcctggcgg gaaatgtggt
    10381 gtgcacatcg caaccgctgg ccgcacaggc ggggcttcgg atgctcgccg acggcggcaa
    10441 cgcggttgac gcggccatcg ccaccgccat cacgctcacc atcgtggaac cagtgtcaaa
    10501 cggcatcggc tcggacgcct tcgccatcgt ctggaacggt aagcgattac acggattgaa
    10561 cgcgtcaggc cgctcgcccg cgggctggac gccagaatac ttcggggaca acgccgttcc
    10621 cgcgctcggg tggaattcgg tgacggtgcc cggggccgta tcggcgtgga ccgagctaca
    10681 cgccaagttc ggaaagctgc ccttcgagaa gcttttcgag ccggcgatct cctacggccg
    10741 caacggcttc ctggtttcgc cgaccgttgc cgaccaatgg gcggcgcagg ttccggtgtt
    10801 caagacccag cccggtttcg ccgaggcgtt cctgcccgcc gggcgcgccc cgaaacccgg
    10861 tgagctgttt aggtttcctg accacgcggc cacgctcgaa ctgatcgccg agacccgcgg
    10921 cgaggcgttc taccgagggg aactggccgt caaactcgag gaacacgcgt cgtccaacgg
    10981 cggggtcctg cgggccggcg acctcgccgc ccaccacgcc gactgggtcg acacgatcca
    11041 cggcaattac cgcggataca cggtgcacga aatgccgccc aacggccagg ggatcgtggc
    11101 actgatcgct ctgggaatcc tggagcactt cgacatgtcc acgttgccag ccgattccgc
    11161 cgacagtgtg catctgcaga tcgaggcgct gaagctggct ttcgccgacg cacatgccca
    11221 tgtcgccgac atcgaccaca tggcgctggc gccggaacgc ctgttggaca gggactacct
    11281 gcagcagcgc gcggcgttga tcgacccgaa gcgggccaag ccggcgtccg cgggcatccc
    11341 aagcggcggc accgtctatt tgacggccgc cgatgccacg ggggtgatgg tgtcgatgat
    11401 ccagtcgaac tacatgggat tcggttccgg tgtggtggta ccgggcacgg gaatctcgct
    11461 acagaaccgg ggcgcgcatt tcgccaccgc tcaaggccat ccgaaccggg tcgggcccaa
    11521 caaacgcccc taccacacga tcatccccgg ctttgtcacc aaagacggtg tcccggtgat
    11581 gagcttcggc gtgatgggcg gcacgatgca gccgcagggc catgtgcagg tgctggcgcg
    11641 catcgcagac cacggtcaga acccgcaggc cgcatgtgac ggcccgagat tccggtgggt
    11701 gcagggcatg caggtcagct gcgaagcagg ctttccgcgg tcaactctgg acgagctgcg
    11761 gcagcgcggg cacgacctga ttgccgtcga cgactacaac caattcggca gctgccaagc
    11821 catctggcgt ctcgacgacg gatatttcgc cgccagcgat ccccgccggg acggccaggc
    11881 cgcggccttc tagactcact gctccctctt gacctaacgc gaaacgaggg gtcagactca
    11941 ataaataaac caaccgtcgg tctaattatg aggttctggt ggcgaggacg gtcaacgttg
    12001 cggaacacgc cgcgcggcgc gatgaaatcc tcgatgccgc acagcggttg atcctgtccg
    12061 ccggctatga gcgcctgacg gttcaggaca tcctcaacga cctgcaaatc tccaaaggag
    12121 ccttctatca ctatttcgac tccaagccga cggtgatcga agccctcacc aggcggttgg
    12181 tcagcgactc cgagcgggct ctcgcgccga ttgccgaaaa ccccgaaatg ggggccgtaa
    12241 cgaaattaca cgccttcttc ggcgagatca tccgctggaa atcggagcgc cagaaccttt
    12301 ttgtggcaat gctgccggcg tggtatgcgc ccgacaacct ggccttccgg ctgctggtgg
    12361 atcgcgagag cgcacaacga ctagggccgc tgctgagcac gatcgtcggt cagggcgtcg
    12421 acgagggcca gttcctgacc gcttatcccg atcaggccgg ggcgatcatc ttcgccatca
    12481 tccaggcgct ccaagacgcg atggcccaga agctgctcga agtccagcaa cggtcaccgt
    12541 cgtcaccgat tgtcaacgac ttggtgaaca cgcatggggc gcacatggaa gcgatcgagc
    12601 ggtacctggg cgtgccgatg ggcacgctga atcgtgttga tgcccgggca gtcaagagtt
    12661 ggatcacggc gttacaccgc gcctacaccg ccgcacacgg gaagcggtaa gcgcgccatg
    12721 gagattcggc aaaccacgtg cctggtagcc ggtggcggac cggctggcat gatgctcgcc
    12781 tacctgctcg cgcggtcggg tgtacagacg actctggtgg agaagcacgg tgacttcctc
    12841 cgagacttcc gtggcgatac ggtccatcct tcaaccaccg aactgatgca cgagctcgga
    12901 cttctcgagg actttctgca gcaggtcccg cacgaggcca tggaacaact gggcatcgga
    12961 gtaaaaggcc agacatacga tctcatcgat ttccgccacc tgcccaccgc ctgcaagtac
    13021 gtcgtcttca tgccgcaatg ggatttcctg aacttcttgg cggacaaggc caagcggtac
    13081 ccgagctttc ggcttgagat gagtaccgaa gtaaccggtc tgctgtggca gggcgatcgc
    13141 gttgccggca tcaccgccca aactgcgggc gatccagtcg agatccgcgc cgacctgacg
    13201 gtagcgtgcg acggacggtg gtcggtcatt cgggagcatt cggggctgcc ggtcacggag
    13261 tttcccatgc ccatcgatgt gctgtggttc cggcttccgc gcattggctc ggctcccgaa
    13321 acactcgggt acctcggcgg cggtcagatc gtcatcgcga tcaaccgtgg cgagtattgg
    13381 cagtgcggca cgatcatcga caaggacggc ttcgatcgag tgcgggccga agggcttcag
    13441 gcattccggt ccagcgttgc ggcggccgcc ccgttcctga ccgccagcct cgccgccttg
    13501 accgactggg accaggtcaa gttgctgtcg gtgcgcgcca accggttacg ccggtggtgg
    13561 ctaccgggct tgctgtgcat tggggacgcc gcacacgcga tgtctccggt cggggggatc
    13621 ggtgtcaact acgccgtcgc cgacgcggtg gccgccgcca acgcactcgc tgagccgctg
    13681 cggaatggac gggtctccgc cgatgatttg cgtgcggtgc agaaacgccg gctcatcccc
    13741 accaggatcg ctcagcggct gcagggcgcc cagacggcga acctcgcccg catgagcaag
    13801 acggagccgc cgctgtggat gttgcggctg ctgagccact gtgcgccggc caagcggttg
    13861 ctcggccgca cggtggggct tggttttcgt cgcgaacaca tccgcaccgt cgagcgcagc
    13921 taaacgctga tcttgccctc tgctgcctct agaccgatgt cgcttcggaa atgactgccg
    13981 ggcaatcgaa ttgagccgag aatctcgtac gcacgcgtgc gtgctgcggc caggtcggcg
    14041 ccggtgccca ccaccgacag cactcggcca ccagacgaga cgatcgcgcc gtcgtcgcgt
    14101 cgcgcggtgc cggcgtgcag caccccctcg gcttcggacc cgacgatgac gtctccgacg
    14161 cgggggcgcc cgggatagtt ctcggccgcc accaccacgg cgacggcggc accgtcgcgc
    14221 cactgcacct cgccgaaatc ggtcagttgg cccgtcgccg cggcatagag caactgcccg
    14281 agcggtgaat ccagcagcgc caacacggcc tgcgtctccg gatcgccgaa gcggcagttg
    14341 aattcgacca ccgccgggcc gcccttggtc atcgcgagtc cgacgtacag caacccgctg
    14401 aatgggctcc cgcgctgaac catctcggcc gcaacgggtt ccacgatctg actgaccaat
    14461 tccccggaca cgccatcggg cagccacggc agcggcgcgt atgcgcccat accgccggta
    14521 ttgggcccgg tgtcaccgtc gccgacccgc ttgaagtcct gtgccgccaa cagcggcacc
    14581 acggtccggc cgtcgacgac acagaacagc gacacctctg gtccgtcgag gtaggactcc
    14641 agcagcaccg ggcgacccgc ctcgagcagc gcggcggcat gggcgcgcgc cgcgtcgcga
    14701 tccggagtca ccaccacgcc cttaccggcg gccaagtggt cgtccttcac cacccaggcg
    14761 gggtccccgg cgcgcggtcc gaaccggtcc agagcagcat ccaaacgcgc cgggctgtcg
    14821 acgatttcac tggtcgcggt gcgcacaccg gccgcggcca tgacctcttt ggcaaacgct
    14881 ttggagcctt cgatgcgggc ggcgtccttg ctgggcccga agcagacgat gcccgcctcg
    14941 cggacggcgt cggccacccc aagcaccaac gggacctcgg gaccgatgac caccatgtcg
    15001 gcccggacct cccgcgccag ggctactacg tcctcgcttg aggtgatatc gacatcgtgc
    15061 tgctcggcca cccgggcggt accggcgttg ccgggagcga tcgccagtcc cgtgacctgt
    15121 ggatccctgc gaagcgccag cagcagcgca tgttcacggg caccggaacc gatcaccagg
    15181 acgcgcacga aacgtcactc tagcgggccg ttgggccggg agggggcgcc aacgtgtcgg
    15241 tcatgccgag ccttagccgc tggaatcacc cgcgtgcatc ttcaacgcgg catccacgtt
    15301 ggccttcttg tcctcatcgg cgcccttcgc cgcggccttc ttgcgctttg ccacgacggt
    15361 gtcgaccgcg ccgttgaggg ccgagcccag cgggaatccg agatagtggg tgaggaagac
    15421 cgccatctcc ttcagctcgg tctcgctcag ttcgccgttg agcagcgcgg cgttgatctg
    15481 gatctcggcc aggtcgcgat ttccgaccgc ggtcaccgcc gtcagcgtca tgatgcgctt
    15541 gtcgcgcatc gacaatcccg accgtgtcca gatactgccg aacaggtggt cgacggtcag
    15601 gtcgaagtac gcgtcgccct cgatgttggg catctcccag ccgtacacct cgttcatctt
    15661 ctcgaggccc ttgcggcgca gttcgtccat catgacgtct ctttctctgt gtgtggaaca
    15721 ccaagtccaa tcgccaaccg ctcaaacgcc aaccgggcca acggcaaatc gaccgacacc
    15781 gcgtcgccca aagccagcgc cagactcagg tccttttccc ctaactcgcg cgtgtgctcc
    15841 agcatgggac gcaacggatc gtccgcggcg aacggggccg tcgtctgccg gaacatgatc
    15901 gcggccgcgc cacccgtgag cccgtcgctg tggcgcacta ccttggcgag ttgccgtagg
    15961 ctcaggccgc ttgtctcggc caacttctcc gcctccgcgg cggccgcgta ggacacgaag
    16021 gtgagcatgt tgcgcgcaag cttcatccga gtgcccgcgc ccggttcgcc ggcatggatc
    16081 accagcgacg cccagcgccc aaaaggttcc ctgagccgct cgaacgattc gtcatccgca
    16141 cccaccatgg tggccagttg cccctcggcg gccgcccccg cgccgccact gaccggagca
    16201 tccacgatgt gaatcccccg cggcttgagt ccccgggcca gttgggcggc ggtggtgtgg
    16261 tcgattgtcg agtgaatcgc gatgatggtg cccggtttag cgcgcgacgc cagtccgccg
    16321 tccccgctga caaccgcgcg cacctgcgcg tcgtcgaaca cggtgacgct gatgatgtcg
    16381 gcttcggcga catcggacag cgtggtcgcg gcgaccgcgc cgccgtcgac gaatggggcc
    16441 atcgcctcgg cgcgcacgtc gaagacggtc agtccgcccg gccagtcgag cagccgcttg
    16501 gccatcggag cgccctgatt gcccagcccg atgtatccca ggcgcggtat gttctccgta
    16561 ttcttgctca tgagcggatt acctgtccgc cgtcgacggc gactttggtg tcgaatcgca
    16621 tgtcttcctc tccgcggcgc gtcaattcgc cttgaccgtg tcagttcgcc actgtggcaa
    16681 tgagtttggc ttccaggtac tcctcgaaac cgagaagacc catctcccgg ccgttcccgg
    16741 attgtttgta gccaccgaac ggcgcatcgg ccgaatacca gacaccgccg ttgacgtcga
    16801 ccgtccccac ccgcagccgc gaggcgatcc tggctgcgcg ctcgctgtcg gcgctaaaca
    16861 cggtgcccga caagccatat ggcgaatcgt tggcgatgcg caccgcgtcg tcgtcgccgt
    16921 cgtgggcgat cacggtgagc accggaccga aaatttcctc ccgggcggac cgcgcgtcgt
    16981 tggacaggcc cgcgatcacg gtgggctcga tgaagaaacc ggcctccttg ttcgccggac
    17041 ggccgccgcc gcacgcgaac gtcccacctt cggcgatggc aaggtcaagg tagccctgca
    17101 cccgatcgcg ctgtcgcgcc gaaatcaagg ggccgcacac ggttccgggc tcgttgggat
    17161 cgccgggctt gatcgacgac atcgtcgcgg cggcgattgc gaccgcctcg tcataacggg
    17221 cacgtggcac caccagccgt gtcgtgatcg cgcagccctg ccccgcgtgc accgctgcgg
    17281 tgaaagccga caccgagcac gccgcgccca ggtcggcgtc gtcgagcacc acgaacgccg
    17341 atttgccgcc caattccagg aacacctttt tgatcgtcgc ggcggcgtcg ctcatcacgc
    17401 tgcggccggt ggctgttgat ccggtgaatg aaatcatgtc cacccgaggg tctttggcca
    17461 agaacgcccc cagggcgtgg tcgcaggagg tgacgatgtt gaccacacct ggcgggaagt
    17521 cggtgtgctc ggcgatgatt tccccgagca ccgcagcgca ccatggtgtg tcgggtgcgg
    17581 gcttcaatac gacggtgttg cccgcagcca gtgctggacc cagcttggcc agattgatct
    17641 ggtgtgggaa gttccacggg gtgatcgcgc cgacgacgcc gacggcttcg cgtgcgatgg
    17701 tgcggcgggt gggaattccc atcggtgccg cctcgccgag atcggagttc caggtgtagg
    17761 actcggctgt atcggcggcg aaccccaggt cgccgaccgg gccctccaat tgcgccgctg
    17821 cggtcagcat ccgtggcgcg ccgacttcgg cgatcgtcag ttctcgcaat tcttcgacat
    17881 gttcgcgcat cg
//
LOCUS       DASSGQ010000287        22117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_385396,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000287 DASSGQ010000000
VERSION     DASSGQ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 22117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22117
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385396"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFI55_12635"
     CDS             <1..202
                     /locus_tag="VFI55_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET7074915.1"
                     /translation="PKTSTFALTNATMPYVLKLADHGWQAACRSDPALAKGLSTHEGM
                     LLSERVAADLNLGYTDPATVLA"
     gene            complement(206..1111)
                     /gene="bla"
                     /locus_tag="VFI55_12640"
     CDS             complement(206..1111)
                     /gene="bla"
                     /locus_tag="VFI55_12640"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HET7074916.1"
                     /translation="MTALSRRRLLAALTASALTACGRAAAVPGPDPADPVYERLDELE
                     RQYNAYIGLFATDLVTGRTLAHRDDDPFAMCSTFKAYAAARVLQQAQRDELDLQQAVF
                     IDPGLLLPNSPVTAPQVGKSMSLAQLCAAALQRSDNAAANLLLRAIGGPPAITEFARS
                     IGDDRTRLDRWEVELNTAIPGDPRDTSTPRALGGGIRNLLTGTVLGESQRSQLEEWMR
                     GNTTSTMRAGLPIGWTTADKTGTGDYGSTNDIGVAYGPDGPGSQRILLSLMTRSQSPN
                     QDADPLGPLIAEVTRSVLPWLTGGG"
     gene            complement(1118..2140)
                     /locus_tag="VFI55_12645"
     CDS             complement(1118..2140)
                     /locus_tag="VFI55_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET7074917.1"
                     /translation="MVLGFWDIMVPIVGAPMAGGPGTPALAAAVSNAGGLGFIPSGYV
                     SAERFADDIADARAMTTGPLGVNLFVPQPSVADWVALDYYADELEGVAEHYQVEVGRP
                     EFGDDDEWEHKLEVVADTRPELVSFTFGVPPPDVIRRLSALGLLVMVTVTSLYEAGVA
                     VAAGADSLVVQGPDAGGHRGTFAPDMEPGTESLHELIDRIHRAHREIPIIAAGGLGTA
                     EDVAGVLRRGAVAAQVGTALLLSDEAGTHRAQRTAMKNPLHGRTIVSRAFSGRYARGL
                     ENTFTRLLNDVAPLGYPEVNQMTLPIREAAADWEDPNGMALWAGTSFRKTRSGPVADI
                     MSSLVG"
     gene            complement(2145..3527)
                     /locus_tag="VFI55_12650"
     CDS             complement(2145..3527)
                     /locus_tag="VFI55_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET7074918.1"
                     /translation="MPLADPGGPSADPPGARRGRAGALRRAKQTAESEHQLRRTTLPG
                     GLRVVTEYLPAVRSASVGVWVGVGSRDEGATVAGAAHFLEHLLFKSTPTRTAVGIAQA
                     MDAVGGELNAFTAKEHTCYYAHVLDSDLELAVDLVADVVLNGRCAAEDVELERDVVLE
                     EIAMRDDDPEDALGDMFLASLFGDHPVGRPVIGTAQSVSAMTRTQLHSFHVRRYTPER
                     MVVAVAGNVDHSEVVALVRAHFGSRLIRGRQPIAPRKGSGRVNGRPGLAMVNRDAEQT
                     HVLLGVRTPGRGWEHRWALSVLHTALGGGLSSRLFQEIRERRGLAYSVYSALDIFADS
                     GALSVYSACLPERFADVMRVTGEVLEAVARDGITEAECRIAKGSLRGGLVLGLEDSSS
                     RMSRIGRSELNYGKHRSIEHTLQQIDAVTVEQVNAVAHRLLSKRFGAAVLGPYTSKRS
                     LPQQLRAMVN"
     gene            complement(3514..5793)
                     /locus_tag="VFI55_12655"
     CDS             complement(3514..5793)
                     /locus_tag="VFI55_12655"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET7074919.1"
                     /translation="MSVAEIDEGVFEATATIDNGSFGTRTIRFETGRLAQQAAGAVVA
                     YLDDENMLLSATTASKSPKEHFDFFPLTVDVEERMYAAGRIPGSFFRREGRPSTDAIL
                     TCRLIDRPLRPSFVAGLRNEIQVVVTILSLDPNDLYDVLAINAASASTQLAGLPFSGP
                     IGGVRVALIDGTWIAFPTVEQLESAVFDMVVAGRIVGEEAGKPDVAIMMVEAEATEKV
                     IELVDGGAQAPTESVVAEGLEAAKPFIAALCTAQQELADSAAKPTGEYPVFPEYGEDV
                     YYSVASVATDELAAALTIGGKAERDQRTDELKAEVLKRLADTYEGREKEVSAAFRSLT
                     KKLVRQRILTDHFRIDGRGITDIRALSAEVAVVPRAHGSALFERGETQILGVTTLDMV
                     KMAQQIDSLGPETSKRYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALIPVLPS
                     VEEFPYAIRQVSEALGSNGSTSMGSVCASTLALLNAGVPLKAPVAGIAMGLVSDDVEV
                     DGKTERRFVTLTDILGAEDAFGDMDFKCAGTKDYVTALQLDTKLDGIPSQVLAGALAQ
                     AKDARLTILEVMAEAISEPDEMSPYAPRVTTIKVPVDKIGEVIGPKGKVINAITEETG
                     AQISIEDDGTVFVGATDGPSAQAAIDRINAIANPQLPTVGERFLGTVVKTTDFGAFVS
                     LLPGRDGLVHISKLGKGKRIAKVEDVVNVGDKLRVEIADIDKRGKISLILVAEEDSAD
                     SSASAPAPSPATAPADAAS"
     gene            complement(6138..6407)
                     /gene="rpsO"
                     /locus_tag="VFI55_12660"
     CDS             complement(6138..6407)
                     /gene="rpsO"
                     /locus_tag="VFI55_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET7074920.1"
                     /translation="MALTAEQKKEILGQYGLHDTDTGSPEAQIALLTKRIADLTEHLK
                     VHKHDHHSRRGLLLLVGRRRRLIKYISQIDVERYRSLIERLGLRR"
     gene            complement(6540..7511)
                     /locus_tag="VFI55_12665"
     CDS             complement(6540..7511)
                     /locus_tag="VFI55_12665"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET7074921.1"
                     /translation="MQRWRGQDEIPTDWGRCVVTIGVFDGVHRGHAELIAHAVKAGRT
                     RNVPTVLMTFDPHPMEVVYPGSHPAQLTTLTRRAELVEEFGIDVFLVMPFTSDFMKLT
                     PERYVHELLVENLHVVEVVVGENFTFGKKAAGNVDTLRKAGERFGFAVESMSLLSEHH
                     SNETVTFSSTYIRSCVDAGDMVAATEALGRPHRVEGVVVRGYGRGAELGFPTANVAPP
                     MYSAIPADGVYAAWFTVLGHGPVTGTVVPGERYQAAVSVGTNPTFSGRTRTVEAFVLD
                     TTADLYGQHVALDFVARIRGQHRFDSVQELVAEIGIDTDRTRTLLTE"
     gene            7657..8349
                     /gene="mntR"
                     /locus_tag="VFI55_12670"
     CDS             7657..8349
                     /gene="mntR"
                     /locus_tag="VFI55_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HET7074922.1"
                     /translation="MRADDGPGGITAVGQDYLKVIWNAQEWSRQKVSTKMLADRIGVS
                     ASTASESIRKLAEQGLVDHEKYGAVTLTEAGRRAALKVVRRHRLLEAFLVNELGYGWD
                     EVHDEAEVLEHAVSDRLVARIDAKLGFPQRDPHGDPIPASDGQVPTPPAKQLWACSAG
                     DSATVARISDADPEMLRYFADVGINLDSRLRVLTRREFAGMISVVIESDDGAESTVDL
                     GSPAARAIWVVR"
     gene            complement(8372..9634)
                     /locus_tag="VFI55_12675"
     CDS             complement(8372..9634)
                     /locus_tag="VFI55_12675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7074923.1"
                     /translation="MIEWSDTDLMVRDAVRQFIDKEIRPHLDELETGALSPYPIARKL
                     FSQFGLDAMAAESVKKVLDRERARQDGRTSEATDENHYGASGFGMSGAPASMVAMLVS
                     ELAGVSIGLLSTATVSLGLGAATIMSRGTLAQKERWLPDLMTLKKIAAWAITEPDSGS
                     DAFGGMKTTVRRSGDGDGAYILNGQKTFITNGPYADVLVVYAKLADSAADPRNRPVLI
                     FVLDAGMPGLTQGKPFKKMGMMSSPTGELFFDNVRLTPDRLLGENEQHGGDGRDSARA
                     NFAAERIGIAMMALGIINECHRLCVDYAKTRTLWGKNIGQFQLIQLKLAKMEIARINV
                     QNMVFQTIDRQKAGKPLTLAEASAIKLYSSEAATDVAMEAVQLFGGNGYMAEYRVEQL
                     ARDAKSLMIYAGSNEVQVTHIAKGLLAD"
     gene            complement(9672..10874)
                     /locus_tag="VFI55_12680"
     CDS             complement(9672..10874)
                     /locus_tag="VFI55_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217306.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HET7074924.1"
                     /translation="MSAKGSNKVYVIGVGMTKFEKPGRRAGWDYPDMARESGTNALHD
                     AGIEYSEIEQGYVGYVYGESTSGQRALYELGLTGIPVVNVNNNCSTGSTALFLAAQAI
                     RGGLADCTIALGFEKMQPGSLAASYEDRTNPMDKHVKAMAEISEFAFPAAPWMFGAAG
                     REHMKQYGSTAEHFAKIGFKNHKHSVNNPYAQFQDEYTLDDILAARMIYDPLTKLQCS
                     PTSDGSGAAILASESFVDSHGLAGQAVEIVGQAMTTDFASTFDGSAKNLIGYDMNVQA
                     AQRVYHQSGLGPEDFQVIELHDCFSANELLLYEALGLCGAGEAPKLIDNGDTTYGGRW
                     VVNPSGGLISKGHPLGATGLAQCAELTWQLRGTADKRQVDDVTAALQHNIGLGGAAVV
                     TAYQRADR"
     gene            11013..11834
                     /locus_tag="VFI55_12685"
     CDS             11013..11834
                     /locus_tag="VFI55_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007240945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HET7074925.1"
                     /translation="MKATVTPEAANAIAEIAQRHGLSQEAVLSMLFALRAGGGTMAQF
                     SIPELGGGGQWMRGGMTMVGDMFDNALKARVDALCNELVQLLATTTVFPPSTAHSEAG
                     LLPSNNWWPAELGVPSAAGGQNDVRYAFFPGTRRLAVQIGGVTRVFDTGEHHIGGVQQ
                     QQGGGYGSVVFTSQLGTFGVSSLRDLGPARAADASAAPPAPGAPHAPQYQSQQQSNPP
                     PQFAPETPPARSASAVPGDSAAIVAAIESLAGLHQRGILSDEEFATKKAELLGRL"
     gene            complement(11905..12801)
                     /gene="truB"
                     /locus_tag="VFI55_12690"
     CDS             complement(11905..12801)
                     /gene="truB"
                     /locus_tag="VFI55_12690"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET7074926.1"
                     /translation="MSESPTGPGLVVVDKPAGMTSHDVVARCRRIFATRRVGHAGTLD
                     PMATGVLVVGVDRATKILGLLTAASKSYAATIRLGQTTSTEDAEGELLHSVSASHLAD
                     EAIAGAIAGLRGEIEQVPSAVSAIKVDGRRAYQLMREGHVVELQARPVRIDRFEMLAT
                     RRAVDGVIDLDVDVDCSSGTYIRALARDLGAALGVGGHLTSLRRTRVGRFGLDQARSL
                     DDLSARPRLSWTLDEACLLMFPRRDLNADEAEAAGNGRSLSPAGIDGVYAASDADGRV
                     IALLRDEGGRTKSVVVIRPATL"
     gene            complement(12798..13484)
                     /locus_tag="VFI55_12695"
     CDS             complement(12798..13484)
                     /locus_tag="VFI55_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HET7074927.1"
                     /translation="MTARMLLSPVLPGTVSEDLAYAEVYSDPPGLSPLPEEEPLIAKS
                     VAKRRNEFITARHCARIALGELGVPPVPILKGDKGEPCWPDGVVGSLTHCTGYRGAVV
                     GRAGPVRSVGIDAEPHDVLPQGVLDAITLEQERHEIAALPEGLHWDRILFCAKEATYK
                     AWFPLTKRWLGFEDAHITFDIDSPGMGTAGVFVSKILIDGETLSGSPLTALRGRWSVE
                     RGLVLTAIVL"
     gene            complement(13481..14458)
                     /locus_tag="VFI55_12700"
     CDS             complement(13481..14458)
                     /locus_tag="VFI55_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7074928.1"
                     /translation="MAWQKSQHESTNGAQPTLWAVSDLHTGHLGNKPITESLHPSSPE
                     DWLIVAGDVAERTDEIRWALDLLRRRFAKVIWVPGNHELWTTNRDPVQIFGKARYEYL
                     VNMCDEMGVVTPEHPFPVWTERGGPATIVPMFLLYDYTFLPEGATSKGEGIAIARERN
                     VVATDEFLLSPEPYATRDAWCRERLSRTRARLEQLDWMTPTVLVNHFPLVREPCEALF
                     IPEFSLWCGTTKTADWHTRYNAICSVYGHLHIPRTTWYDGVRFEEVSVGYPREWRRRK
                     PYSWLRQVLPEPRYAPGYLNDFGGHFVVTPEMRRQAEQFRERLRQRQSR"
     gene            14641..15243
                     /locus_tag="VFI55_12705"
     CDS             14641..15243
                     /locus_tag="VFI55_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 domain-containing protein"
                     /protein_id="HET7074929.1"
                     /translation="MSANVRLIGALGALVTTVIGGAMGLRGAPSASTGARPIELRSTA
                     EPMETTAPTETTMKSPIVATTDPRPFDACEDIPFDVIQRLGLAFTPPEPEDGLRCHFD
                     AGNYQMAVEPIVWRTYAQTLPADAVETTIQGHRAAQYWVLKPTYHNSYWYYSCMVAFK
                     TSYGVLQQALFFSTVYSNPDVDCMSTNLQRANDLAPYYKF"
     gene            15322..17028
                     /locus_tag="VFI55_12710"
     CDS             15322..17028
                     /locus_tag="VFI55_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET7074930.1"
                     /translation="MTTTATRPAQRRLGALRQVGGNAVGRLLGLPPAATGYTVKQVQV
                     PMRDGVQLLADHYAPTTAEPRGTLLVRGPYGRAFPFSLAYARLYAARGYHVVLQSVRG
                     TFGSGGVLEPMVNEVSDGADTVDWLREQHWFTGRFATVGVSYLGFTQWALLVDPPPEL
                     AAAVVTVGPHDFYDAVWGTGTFAINDFLGWSHVVSQQEDPARIRGRIRQLGAPRRVAR
                     AVAEVPVGAGARALLGTGAPWFESWVEHSDPEDPFWKSLRLGQALDRVQVPVLLLSGW
                     QDIFVQQTLQQYAHLRGRGVDVALTVGPWTHTEMLTKGLAVGARDTLDWLDAHLGGTE
                     ALRRPSRVRVFVTGLGWRNLQDWPPATTERALYLRPGGYLGETEPTDLTGVAPAKFRS
                     DPTDPTPTTGGPLLSPDAGYGDDSKLARRADVLAFTSGALAQDLTVYGSPVVELAHTS
                     DNPCADLFVRVSQVDAKGRSRNVSEAYRRLGPGTETVRIELDGIAHRFPAGTRIRLLI
                     AGSWFPRYARNLGTGEPVLTGRHSQPVTHVVHYGRSRLLLPAGPADPSADRVADPGSD
                     GS"
     gene            complement(16987..17568)
                     /locus_tag="VFI55_12715"
     CDS             complement(16987..17568)
                     /locus_tag="VFI55_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014712056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HET7074931.1"
                     /translation="MTLTATMPALKEWAAVVHGLLAGRQRVLLRKGGIGEKRFELAAR
                     EFFLFPTVAHSHAQRVRPEHRDLLEAGATDSTDDRLLVRAAAKVVAAVPVNRPEGLPA
                     IEDLHIWTAESVRADRLDFRPKHKLAVLVVSVIPLAEPVLIARTPEYAGCTSWVQLPL
                     TPADLVSVRPAHDPVHDDAALAAVAARVRDAVG"
     gene            complement(17565..17834)
                     /locus_tag="VFI55_12720"
     CDS             complement(17565..17834)
                     /locus_tag="VFI55_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_007411535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 family protein"
                     /protein_id="HET7074932.1"
                     /translation="MCRNITELRGLQPSATPEEIAAAARQYVRKVSGITHPSAVNAEA
                     FEAAVAEVTETTTRLLGALAPRRQPPKTVPPLRRPEVVARLAGAK"
     gene            17977..18573
                     /locus_tag="VFI55_12725"
     CDS             17977..18573
                     /locus_tag="VFI55_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074933.1"
                     /translation="MRAGPLTVQALTVRALGGLTLAAASAIAGPAAQAGAQPPPGFPN
                     LDTFAAVAADGYVTMSDPGASPRVSFSPTSTLVCTFYGGPAPPPQPSQDIKCNGDMPG
                     MDDVPFPGGGHTKPGDCVVGAVNFKGPGYELSRMSYGGCDGSSPPLPSGGKSLGAGQK
                     LSYLNVTCAVGADNLVACLDATSGDHGFVLQRSGSWAF"
     gene            complement(18581..19591)
                     /locus_tag="VFI55_12730"
     CDS             complement(18581..19591)
                     /locus_tag="VFI55_12730"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET7074934.1"
                     /translation="MTTTNPKTELAGTSCVGARVDALGAVELLSCAATIAVIAHVHPD
                     ADTIGAGLALALVLDRCGKDVEVSFASPATLPESLSSLPGCELLVTPDAMRRDVDLVV
                     TVDVPSLKRLGELSDLAAPGRQLLVIDHHASNDMFGTANFIDVSADSTTMMIAELLDT
                     WGKPIDLEVAHCIYAGLTTDTGSFRWASARALRLAARLVDTGVDNAAISRTLMDTHPF
                     GWLPLLSRVLGSAQLLREAAGGHGLVYAVVENRDWIDSRPEEVESIVDIVRTTAQAEV
                     AAVFKEIAPQQWSVSMRAKADMDLATVASTFGGGGHRLAAGYSTAGSIEDVVASLCAA
                     LG"
     gene            complement(19566..20075)
                     /gene="rbfA"
                     /locus_tag="VFI55_12735"
     CDS             complement(19566..20075)
                     /gene="rbfA"
                     /locus_tag="VFI55_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET7074935.1"
                     /translation="MADPARARRLAKRIFTIVASAIEFEIKDPGLDGVTIVDTKVTAD
                     LHDATVFYTVMGPTLDDEPDYAAAAAALERAKGTLRTRVGAGTGVRFTPTLTFTRDTT
                     SDSVHRMDELLARAHAADADVARVRSGAKPAGEADPYRVSGTFAEPQAGALDGLDARD
                     AGDDDQSED"
     gene            complement(20075..>22117)
                     /gene="infB"
                     /locus_tag="VFI55_12740"
     CDS             complement(20075..>22117)
                     /gene="infB"
                     /locus_tag="VFI55_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET7074936.1"
                     /translation="GGGGVGAAPGGFRGRPGGGGPGGGGPGGPGGGGGGRPGQRGGAA
                     GAFGRPGGAPRRGRKSKRQKRQEYDSMQAPVVGGVRLPHGNGETIRLARGASLSDFAE
                     KIDANPAALVQALFNLGEMVTATQSVGDETLELLGGEMNYNVQVVSPEDEDRELLESF
                     DLTYGEDEGGEEDLQTRPPVVTVMGHVDHGKTRLLDTIRKANVREGEAGGITQHIGAY
                     QVAVDLDGTERPITFIDTPGHEAFTAMRARGAKATDIAVLVVAADDGVMPQTVEAINH
                     AQAADVPIVVAVNKIDKEGADPTKIRSQLTEYGLVAEDFGGETMFVDISAKQGTNIEA
                     LLEAVVLTADAALDLRANPDMEAQGVAIEAHLDRGRGPVATVLIQRGTLHVGDSVVAG
                     DAYGRVRRMVDEHGEDIEAALPSRPVQVIGFTSVPGAGDNFLVVDEDRIARQIADRRS
                     ARKRNALAARSRKRISLEDLDSALKETSQLNLILKGDNAGTVEALEEALLGIQIDDEV
                     ELRVIDRGVGGITETNVNLASASDAVIIGFNVRAEGKATELANREGVDIRYYSVIYQA
                     IDEIEKALRGLLKPIYEENQLGRAEIRALFRSSKVGLIAGCLVTSGVIRRNGKARLLR
                     DNIVVAENITIASLRREKDDVTEVRDGYECGLTLTYSDIKEGDVIESFELVQKERA"
BASE COUNT         3476 a         7691 c         7267 g         3683 t
ORIGIN      
        1 gccgaagacg tccaccttcg ctttgaccaa tgcgacgatg ccgtatgtgc tcaaactcgc
       61 cgaccacggc tggcaggcgg catgccgttc ggatcccgct ctggccaaag ggctttcgac
      121 gcacgagggg atgctgctgt ccgagcgggt cgccgccgac ctgaatttgg ggtacaccga
      181 tccggccacc gtattggcct gacgctcagc cccctccggt cagccacggc agcaccgacc
      241 tggtcacctc ggcgatcagc ggccccagcg gatcggcgtc ttggttcggc gactgggagc
      301 gcgtcatcag cgaaagcagg atcctctggc tgcccggtcc gtcgggtccg taggcgacgc
      361 cgatgtcgtt ggtgctgccg tagtccccgg tgccggtctt gtcggcggtg gtccacccga
      421 taggtagacc cgcgcgcatg gtcgaggtgg tattgccgcg catccactcc tccagctggc
      481 tgcgctgcga ttcgccgaga acggttcctg tgagcaggtt tcggatcccg ccgcccaggg
      541 cccgcggggt gctggtatcg cgcgggtcgc cgggtatcgc ggtgttgagt tcgacctccc
      601 agcggtccaa cctggtgcgg tcgtcaccga tggagcgagc gaattctgtg atggccggcg
      661 gccccccgat cgcccgcaac agcaggttgg cagccgcgtt gtcgctgcgc tgcagcgccg
      721 ccgcgcagag ttgcgccagg gacatgctct tgccgacctg cggcgctgtc accggcgagt
      781 tgggcagcag cagcccggga tcgatgaaaa cggcctgctg caaatccaat tcgtcacgct
      841 gggcctgctg taacacccgc gccgccgcgt aggccttgaa ggtcgaacac atcgcgaacg
      901 ggtcatcgtc gcgatgcgcc aaggtgcgcc cggtcaccaa atcggtggcg aaaagcccga
      961 tgtacgcgtt gtactggcgc tccagttcat cgagccgttc gtagaccgga tcggcggggt
     1021 cgggtccggg gacggcggca gcccgcccgc aggcggtgag tgccgacgcc gtcagggcgg
     1081 ccaacagccg ccgacgtgac agtgccgtca ccgggcgcta gccgaccaag ctggacatga
     1141 tgtcggccac cggcccggag cgggtcttcc ggaacgacgt cccggcccac agggccatcc
     1201 cgttcgggtc ttcccagtcg gccgctgcct cccggatcgg cagtgtcatc tggttgacct
     1261 cgggatagcc cagcggcgca acgtcgttca gcagacgagt gaacgtgttc tccaggccgc
     1321 gtgcgtaccg gcccgagaac gcacgcgaga cgatggtgcg gccgtggaga ggattcttca
     1381 tagccgtccg ctgcgcgcgg tgggtgcccg cttcgtcgct gagcagcagc gcggtgccga
     1441 cctgtgcggc cacggctccc ctgcgcagca cgccggcgac gtcctcggcg gtgcccagcc
     1501 cacccgccgc gatgatcggg atctcgcggt gtgcccggtg gatccgatcg atcagctcgt
     1561 gcagtgactc cgtaccgggc tccatgtcgg gcgcaaaggt gccgcgatgc cctccggcgt
     1621 ccggcccctg gaccaccagg ctgtccgcgc cggccgcgac ggcgaccccg gcctcgtaga
     1681 gcgacgtcac cgtgaccatc accaacaggc cgagcgcact cagccgccgg atcacgtcgg
     1741 gcggcggcac gccgaaggtg aaggacacca actccgggcg agtgtcggcc accacctcga
     1801 gcttgtgctc ccattcgtcg tcgtcaccga attcgggccg accgacctcg acctggtaat
     1861 gctcggcgac gccttcgagt tcgtcggcgt agtagtcgag tgccacccag tcggcgacgc
     1921 tcggttgcgg cacaaacagg ttgacaccca gcggcccggt cgtcatggcg cgagcgtcgg
     1981 cgatgtcgtc ggcgaaccgc tcggcgctga cgtacccgct ggggatgaaa ccgagcccgc
     2041 ccgcgttgga caccgccgcg gccagcgccg gggtgcccgg gccgcccgcc atcggcgcac
     2101 cgacgatggg gaccatgatg tcccaaaagc ccagtaccat ccggctaatt taccatcgcc
     2161 cgaagttgtt ggggcagcga tcgtttcgag gtatacggac ccagaactgc ggcgccgaag
     2221 cgcttgctca gcagccggtg tgccaccgcg ttgacctgct cgacggtcac cgcgtcgatc
     2281 tgttgcaggg tgtgctcgat gctgcggtgt ttgccatagt tcaattcgct gcggccgatg
     2341 cggctcatcc gcgagctgga atcctccagc ccaaggacga gcccgccgcg caacgagcct
     2401 ttggcgatgc ggcattccgc ctcggtgatg ccgtcgcgag ccaccgcttc gagcacctct
     2461 ccggtgactc gcatgacgtc ggcgaaacgc tcgggcaggc acgccgagta caccgacagc
     2521 gccccgctgt cggcgaagat gtcgagcgcc gaatacaccg agtaggccag cccccgtcgt
     2581 tcccgaatct cctggaagag cctggaactc aggccgccac ccagcgcggt gtgcagcacc
     2641 gacagtgccc agcggtgctc ccagccacgt cccggcgtgc ggacgcccaa caacacgtgg
     2701 gtctgctcgg catcccggtt gaccatcgcc aatccggggc ggccgttgac ccgcccacta
     2761 cccttgcgcg gcgcgatggg ctggcgccca cggatcagac gcgacccgaa gtgcgcgcgc
     2821 accaacgcga ccacctcgct gtggtcgaca ttgccggcca ccgcgacgac cattcgttcc
     2881 ggggtgtagc gccgcacatg aaacgagtgc agctgggtcc gcgtcatcgc cgacaccgat
     2941 tgcgcggtgc cgatcaccgg gcggcccacc gggtggtcac cgaacagcga ggccaagaac
     3001 atgtcgccca gcgcgtcctc gggatcgtcg tcgcgcatgg cgatctcctc gaggacgacg
     3061 tcgcgttcca gctccacgtc ctcggcggcg cagcggccgt tgagcaccac atcggcgacc
     3121 aggtccacgg ccagctcgag atcgctgtcg aggacatgcg cgtaatagca ggtgtgctcc
     3181 ttggcggtga acgcgttcag ctcgccgccg acggcgtcca tcgcctgcgc gatacccacg
     3241 gccgtgcggg tcggggtcga cttgaacagc agatgctcga ggaagtgcgc cgctcccgcc
     3301 acggtcgcac cctcgtcacg cgatccgacg cccacccaga caccgaccga cgctgaacgc
     3361 accgcgggca ggtattcggt gaccacccgc aggccgcccg gcagtgtggt gcggcgcagc
     3421 tggtgttcgg attcggcggt ctgcttcgcc cgccggaggg cgcccgcgcg accgcgacgc
     3481 gccccgggtg ggtcggctga cggcccgcca ggatcagcta gcggcatcgg ctggcgcggt
     3541 cgccggggac ggtgccggag ccgaggctga gctgtctgcc gagtcctcct cggccaccag
     3601 gatcaaggag atcttgcccc gcttgtcgat atcggcgatc tcgacccgca gcttgtcgcc
     3661 gacgttcacg acgtcctcga ccttcgcgat gcgcttgccc ttgccgagtt tggagatgtg
     3721 caccagaccg tcacgacccg gcagcagcga cacgaacgct ccgaaatccg ttgtcttgac
     3781 cacggttcca aggaaacgct cgcctaccgt cggcagctgc gggttggcga tggcgttgat
     3841 ccggtcgatc gcggcctgcg ccgagggccc gtcggtggcg ccgacgaaca cggtgccgtc
     3901 gtcctcgatg gagatctgcg caccggtctc ctcggtgatg gcgttgatga ccttgccctt
     3961 ggggccgatg acctcgccga tcttgtccac cgggaccttg atggtggtca cccgcggcgc
     4021 gtaggggctc atctcgtcgg gctcggagat ggcctcggcc atgacctcga gaatcgtcag
     4081 gcgcgcgtct ttcgcctgcg cgagagcacc tgccagcacc tgtgagggaa taccgtcgag
     4141 cttggtgtcc agctggagcg cggtgacgta gtccttggtg ccggcacact tgaagtccat
     4201 gtcgccgaac gcatcctcgg ctccgaggat gtcggtcagc gtgacgaagc gacgctcggt
     4261 cttgccatcc acctcgacgt catcggaaac caggcccatc gcgatgcccg cgaccggcgc
     4321 cttgagcggc accccggcgt tgagcagcgc cagcgtggac gcgcagaccg accccatcga
     4381 ggtcgagccg ttggagccca gcgcttccga cacctgacgg atggcgtagg ggaattcctc
     4441 gacgctgggc agcaccggga tcagggcccg ctcggcgagg gcgccgtgcc cgatctcacg
     4501 ccgcttgggc gagccgaccc tgccggtctc gcccgtggag aacggcggga agttgtagtg
     4561 gtgcatgtac cgcttggacg tctcgggtcc gagcgagtcg atttgctggg ccatcttgac
     4621 catgtcgaga gtggtcacac ccaggatctg ggtctcgccc cgctcgaaca gcgcgctgcc
     4681 gtgcgcccgc ggcaccacgg cgacctcggc cgagagcgcg cggatgtcgg tgatgccccg
     4741 gccgtcgatg cggaagtggt cggtcaggat gcgctggcgg acgagcttct tggtcagcga
     4801 gcggaacgcg gcgctgacct ccttttcgcg gccttcgtag gtgtcggcca gccgcttcag
     4861 cacctcggcc ttcagctcat cggtgcgctg gtcgcgttcg gccttgccgc cgatggtcag
     4921 cgcggctgcc agctcgtcgg tggccaccga ggcaaccgag tagtacacgt cctcgccgta
     4981 ctcggggaag accgggtact caccggttgg tttcgcggcc gagtcggcca gctcctgctg
     5041 ggcggtgcac agcgcggcga tgaacggctt ggccgcctcg agtccttcgg ccacaacgct
     5101 ttccgtcggc gcctgggcgc cgccgtcgac cagctcgatg accttctcgg tggcttcggc
     5161 ttccaccatc atgatggcca cgtcaggctt gcccgcctct tcgccgacga tgcggccggc
     5221 caccaccatg tcgaacacgg ccgactcgag ctgctccacg gtggggaagg caatccaggt
     5281 gccgtcgatc agcgccaccc ggacgccgcc gatggggccg gagaacggca gcccggccag
     5341 ctgggtggag gccgacgcgg cgttgatcgc cagcacgtcg tagaggtcgt tggggtccag
     5401 gctcaaaatc gtcaccacga cctggatctc gttgcgcagg ccggccacga acgacgggcg
     5461 cagcgggcgg tcgatgagcc ggcaggtcag gatcgcgtcg gtggaaggcc gaccttcacg
     5521 ccggaagaac gaaccgggga tgcggccggc agcgtacatc cgctcctcga catcgacggt
     5581 cagcgggaag aagtcgaagt gctccttggg gctcttgctc gcggtggtcg ccgacagcag
     5641 catgttctcg tcgtcgaggt acgcgaccac ggcgccggcg gcctgctggg ccaaccggcc
     5701 ggtctcgaaa cggatggtgc gggtgccgaa gctcccgttg tcaatggtgg cggttgcctc
     5761 gaacacgccc tcgtcaattt cagctacaga cataggtgtc cgtatggcct ctctcaagtt
     5821 attcggcttt ttcgcgtcgt cacgcgcaac tcaacttggg aggcggccca acagctcctc
     5881 agagctgatg cctgaatacg gctacggcca tcgatcgaag cggccgacct gccccagatc
     5941 cggggagccc ggcagccact accgaagacc gcccgatgca gacagggctg ctcccagcaa
     6001 catgcactga cgcgccggaa cggaacgcgc ggatctgcgc ggaccgcacc gtttgctcgg
     6061 gccgcacggg cccagaacgc tcccactcta cacggggcaa ccgggcctgc cggcaagggc
     6121 tctggcttgc ccgagtgtca gcggcgcagg cccagccgct cgatgagcga gcgataacgc
     6181 tcgacgtcga tctgggagat gtacttgatc agccgacgcc ggcgccccac caacagcagc
     6241 agtccccgcc gcgagtggtg gtcatgcttg tgcaccttga ggtgctcggt caggtccgcg
     6301 atccgcttgg tcagcagcgc gatctgcgct tccggcgatc cggtgtcggt gtcgtgcagg
     6361 ccgtactggc caagaatttc tttcttctgc tcggctgtaa gcgccacgaa atatctccat
     6421 caattggtcc gcgatcattg gatttcaagg cgcggccacc gcgaaccgca gcacgcgccg
     6481 atgtcgtccg gcagtttagc agccgggcgg ttaccggccc caatcgtcgc cgggcgtgat
     6541 cactcggtca gcagggtccg cgtccggtcg gtgtcgatgc cgatctcggc gaccagctcc
     6601 tgcacggagt cgaacctgtg ctgcccgcgg atgcgggcga caaagtccag cgcgacgtgc
     6661 tgtccgtaca ggtcggcggt ggtgtcgagg acgaacgcct cgacggtgcg ggtgcggccg
     6721 gaaaacgtcg gattggtgcc gacggacacg gcggcctggt agcgctcccc cgggacgacg
     6781 gtgcccgtca ccggcccgtg tccgagcacg gtgaaccagg ctgcatacac gccatcggcc
     6841 gggatggccg aatacatcgg cggcgccacg ttcgcggtgg gaaagcccaa ctcggcgcct
     6901 cgcccgtaac cgcggaccac gacaccctcg acgcggtgtg ggcggcccag cgcctccgtg
     6961 gcagccacca tgtcgccggc gtccacgcag gaccggatgt aggtggacga gaacgtcacg
     7021 gtttcgttgc tgtggtgctc cgacaacagc gacattgact ccaccgcgaa tccgaaccgc
     7081 tcaccggcct tgcgcagggt gtcgacgttg ccggccgctt tcttaccgaa ggtgaagttc
     7141 tcgcccacca cgacctcgac cacgtgcagg ttttccacca gcagctcgtg cacatagcgt
     7201 tcgggggtga gcttcatgaa atcgctggtg aacggcatca ccaggaagac gtcgatgccg
     7261 aactcttcga cgagctcggc gcgccgggtc agcgtcgtca gctgcgccgg gtggctgccg
     7321 ggatagacca cttccatcgg gtgcgggtcg aacgtcatca gcaccgtcgg cacatttcgc
     7381 gtccggccgg ctttcaccgc atgcgcgatc agctcggcgt ggccgcgatg cacaccatcg
     7441 aacactccga tggtgactac gcacctgccc cagtcggtcg gaatctcgtc ttggccgcgc
     7501 caccgctgca cgaccgcaag cctacggcgc gacgcggccc gacgtgaccc gagaaccgat
     7561 atcggtcggt acctaccgca ggtttttgca cgcacaatcg atcaggatgg cgaggagcgc
     7621 accggatcac gcccaggttg cctaaacttt ctgcttgtga gggctgacga tgggcctggc
     7681 ggtattaccg cggtcggcca ggactatctc aaggtcatct ggaatgccca ggagtggtcc
     7741 aggcaaaagg tcagcaccaa aatgcttgcc gaccggatcg gggtgtcggc cagcacggcc
     7801 tcggagtcga tccgcaaact cgccgagcag ggcctggtcg accacgagaa gtatggcgcc
     7861 gtgacgctga ccgaggcggg acgccgggcc gcgctcaagg tggtgcgccg gcaccgcctg
     7921 ttggaggcat tcctcgtcaa cgagctcggg tacggctggg acgaggttca cgacgaggcc
     7981 gaggtgctcg agcacgcggt ctcggaccgc ttggtggccc gcatcgacgc caagctgggg
     8041 ttcccgcaac gcgacccgca cggcgacccg atcccggcct ccgacgggca ggtgcccaca
     8101 ccaccggcca aacagctgtg ggcgtgcagc gcgggagact ccgcgaccgt ggcccgtatc
     8161 tcagacgccg acccggagat gctgcgctat ttcgccgacg tcggtatcaa tctcgactct
     8221 cggctgcgcg tgctgactcg ccgcgagttc gccggaatga tttcggtggt catcgaatcc
     8281 gacgacggcg ccgaaagcac cgtcgatctg ggcagcccgg cggcccgggc gatctgggtg
     8341 gtgcgctgac ccacctgatt ttccgcgagc gctagtcggc cagcaggccc ttggcgatgt
     8401 gcgtcacctg aacctcgttg ctgccggcgt agatcatcag cgacttcgcg tcgcgggcca
     8461 gctgctcgac ccggtactcg gccatgtagc cgttaccgcc gaagagttgt acggcctcca
     8521 ttgcgacgtc ggtggccgcc tccgacgaat acagcttgat cgccgatgcc tcggccagcg
     8581 tcagcggttt tccggccttt tgccggtcga tggtctgaaa caccatgttc tgcacgttga
     8641 tccgcgcgat ctccatcttg gccagcttga gctggatcaa ttggaattgc ccgatgttct
     8701 tgccccacaa ggtgcgggtc ttggcgtaat ccacgcacag ccggtggcat tcgttgatga
     8761 tgcccagggc catcatggcg atcccgatcc gttcggcggc gaagttggct cgggcgctgt
     8821 cgcggccgtc cccgccgtgc tgttcgtttt cgccgaggag gcggtccggg gtcagccgga
     8881 cgttgtcgaa gaacagctca ccggtcggcg aggacatcat gcccatcttc ttgaacggct
     8941 tgccctgggt cagccctggc atgcccgcgt ctagcacgaa gatcagcacc ggccggttgc
     9001 gcgggtcggc cgcggagtcg gcgagtttgg cgtagaccac cagcacgtcg gcgtaggggc
     9061 cgttggtgat gaacgtcttc tgcccgttga ggatgtaggc cccgtctccg tcgccggagc
     9121 gccggacggt ggtcttcatg ccgccgaacg catccgagcc ggagtccggc tcggtgatgg
     9181 cccacgccgc gatctttttc agcgtcatca ggtcgggcag ccagcgctcc ttctgggcca
     9241 gcgtgccgcg gctcatgatg gtcgccgcac ccagcccgag gctgaccgtc gcggtgctca
     9301 gcaatccgat gctcaccccg gccagttcgg acaccagcat cgcgaccatc gatgccggtg
     9361 ccccgctcat tccgaaaccg cttgcgccgt agtgattttc gtcggtcgcc tcgctggtcc
     9421 tcccgtcctg cctggcgcgc tcccggtcca gcaccttctt gaccgattcg gcagccatcg
     9481 catcaaggcc gaactggctg aacagcttgc gggcgatcgg atagggcgac aacgcgcccg
     9541 tttccagttc gtccagatgc ggacgaattt ccttgtcgat gaactgacga acggcatccc
     9601 ggaccatcag atcggtgtcg gaccactcga tcacggctct ccccccttgg ccccgtgcgc
     9661 cgcctcgtga ttcagcggtc ggcgcgctgg taggcggtca ccacggcggc accgcccagc
     9721 ccgatgttgt gctgcaacgc cgcagtcacg tcatcgacct ggcgcttgtc ggcggtcccc
     9781 cgcagctgcc aggtgagttc tgcgcactgc gccaatccgg tcgcacccag cgggtgcccc
     9841 ttggagatca gtcccccgga cgggttgacc acccagcgcc cgccgtaggt ggtgtcgccg
     9901 ttgtcgatca acttcggcgc ctcgcccgcg ccgcacaggc caagggcctc atacagcagc
     9961 agctcgttgg ccgagaagca gtcgtgcagt tcgattacct gaaagtcctc gggacccaat
    10021 cctgattggt ggtaaacacg ttgtgcggct tgcacattca tgtcatagcc gatcaggttc
    10081 ttggcgctgc cgtcgaaggt ggacgcgaag tccgtcgtca tggcctggcc gacgatttcc
    10141 accgcctggc cggccagccc gtggctgtcg acgaacgact cggacgccag gatcgccgca
    10201 cccgaaccgt ccgacgtcgg cgaacactgc agcttggtca gcgggtcgta gatcatccgc
    10261 gccgccagga tgtcgtcgag ggtgtactca tcctggaatt gcgcatacgg gttgttcacc
    10321 gagtgcttgt ggttcttaaa gccgatcttg gcgaagtgct cggcagtgct gccgtattgc
    10381 ttcatgtgtt cgcgcccggc ggccccgaac atccacggcg cagcggggaa ggcgaactcg
    10441 ctgatctcgg ccatcgcctt gacgtgcttg tccatcgggt tggtgcggtc ttcgtaggac
    10501 gccgcgagcg acccgggctg catcttctcg aaccccaaag cgatggtgca gtcggccaat
    10561 ccgccgcgaa tcgcctgcgc cgccaggaac agcgcggtgg aacccgtcga gcagttgttg
    10621 ttgacattga cgacgggaat gccggtcaag cccagctcgt agagcgcccg ctgccccgac
    10681 gtcgattcgc cgtagacata tccgacgtag ccttgctcga tctccgagta ctcgatgccg
    10741 gcgtcgtgca gcgcgttggt ccccgactcc ctcgccatgt ccgggtaatc ccagcccgcg
    10801 cggcggcccg gcttctcgaa tttcgtcatg ccgacaccga tgacgtacac cttgttggat
    10861 cccttggcgg acatagcgcc ccttccagac ggctgggctt ctttgacacc cgtctagtcg
    10921 gcagcttaac cgtgcgcggc cgccggctgg aacccccgcc ctcccgtccg gcttgcgtac
    10981 gctcgaatga agccgacgaa agaatcgaga ctatgaaggc aacggttacc cccgaagccg
    11041 ccaacgccat cgccgagatc gcccagcgcc atgggctgtc ccaagaagcg gtgttgtcca
    11101 tgctgttcgc gctgcgcgcc ggtggcggca ccatggccca gttctcgatt cccgagctcg
    11161 gcgggggcgg gcaatggatg cgtggcggca tgacgatggt cggcgacatg ttcgacaacg
    11221 cgctcaaggc ccgcgtcgat gcgctgtgca acgaactggt tcagctgctt gccaccacca
    11281 cggtatttcc gccctcgacc gcgcactccg aggcggggct gttgccttcg aacaactggt
    11341 ggccggccga actcggggtc cccagtgcgg cgggcgggca gaacgacgtg cgctacgcat
    11401 tcttccccgg aacacgacgg ctggcggtcc agatcggcgg tgtcacaagg gttttcgaca
    11461 ccggcgagca ccacatcggc ggtgtgcaac agcagcaggg cggcggttac ggctcggtgg
    11521 tcttcaccag ccagttgggc accttcggcg tatcgagtct gcgtgacctc ggtccagcgc
    11581 gtgcggctga cgcttcggcc gccccacccg ctcctggcgc gccgcacgcc ccgcaatatc
    11641 agtcgcagca gcaatccaac ccgccgccgc aattcgcgcc cgagacgccg ccggccaggt
    11701 cagcttccgc cgtgcccggg gattctgctg ccatcgtcgc ggccatcgaa tcgctcgccg
    11761 gattgcatca gcgcggcatc ctctccgacg aagagttcgc cacgaagaag gccgagctgc
    11821 tcggccgact ctgacctgcc tgactagagc gtcgccgggc taggccagat tgcccggctt
    11881 ctcctcccgc cgctgcgtgc ctgactagag cgtcgccggg cggatcacca ccaccgactt
    11941 ggtcctgccg ccctcatcgc gcagcagtgc gatcacccgg ccgtcggcgt cgctggcggc
    12001 gtacacaccg tcgatgccgg caggcgaaag cgaccggccg ttgccggccg cctcggcttc
    12061 gtcggcgttg agatcgcggc gcggaaacat cagcaggcac gcctcgtcca gagtccaact
    12121 aagtcggggt cgcgcggaca ggtcgtccag tgagcgtgcc tggtccagtc caaagcgccc
    12181 gacacgggtg cgccgcaacg aagtgagatg cccgccgaca ccaagcgcgg cacccaaatc
    12241 gcgggccagc gcacggatat aagttcccga cgaacaatcg acgtcgacat ccagatcgat
    12301 aacaccgtcg accgcgcgcc gcgtagccag catctcgaag cgatcgatgc gcaccggccg
    12361 ggcctgcaac tccaccacgt ggccctcgcg catcagctgg taggcacgac ggccgtccac
    12421 cttgatcgcg ctgaccgccg acggcacctg ctcgatctca ccgcgcagtc cggcgatcgc
    12481 gccggcgatc gcctcgtctg ccagatgcga ggccgaaaca ctgtgtagca gttcaccttc
    12541 ggcatcctcg gtggacgtgg tctggcccag ccggatagtg gccgcatacg acttcgaggc
    12601 cgccgtcaac agaccgagga ttttggtggc gcggtccacc ccgaccacca gcacaccggt
    12661 ggccatcgga tccagcgtcc cggcgtgccc gaccctgcgg gtggcaaaga tgcggcgaca
    12721 gcgcgccacg acgtcgtgac tggtcatccc cgccggcttg tcgaccacca ctagtccggg
    12781 gccggttggg ctttcgctca tagcacgatc gcggtcagca ccagtccgcg ctcgaccgac
    12841 catcggcccc gcagcgcggt cagcggcgaa cccgacagcg tctccccgtc gatcaggatc
    12901 ttggagacga acaccccggc ggtccccatt ccgggcgaat cgatgtcgaa cgtgatgtgg
    12961 gcgtcctcga aacccaacca gcgcttggtc aacggaaacc aggctttgta tgttgcttcc
    13021 ttggcgcaga acaggattcg gtcccaatgc agcccctcgg gcaacgcggc gatttcatga
    13081 cgttcctgtt cgagcgtgat cgcatcgagc actccctgcg gcaacacgtc gtgcggttcg
    13141 gcgtcgatgc ccaccgaacg caccgggccg gcgcgtccca ccaccgcgcc gcggtaaccc
    13201 gtgcagtggg tgaggctgcc caccacgccg tcgggccagc acggttctcc cttgtcgccc
    13261 ttgaggatcg gcaccggcgg cacgcccagc tcgcccaacg cgatccgcgc acagtgacgg
    13321 gcggtgatga actcgttgcg ccgcttggca accgacttgg cgatcagtgg ctcctcctcg
    13381 ggcagcgggc tcaggccggg cggatcggaa tacacctccg cgtatgccag atcctcggac
    13441 accgtgccgg gcaacaccgg cgacaacagc atgcgcgccg tcatcgtgac tgccgctgcc
    13501 gcaaccgttc ccggaactgt tcggcctgtc ggcgcatctc gggagtgacc acgaagtgac
    13561 cgccgaagtc gttgagatag ccgggcgcgt accggggctc cggcagcacc tggcgcagcc
    13621 agctgtacgg cttgcgccgc cgccactctc gcggatagcc caccgacacc tcttcgaagc
    13681 gcacgccgtc ataccaggtg gtccgcggaa tgtgtaagtg tccatacacc gaacagatgg
    13741 cgttatagcg cgtgtgccag tcggcggtct tggtggttcc gcaccacagc gagaattccg
    13801 gaataaacaa cgcctcgcag ggctcgcgca ccagcgggaa atggttcacc aggaccgtcg
    13861 gtgtcatcca gtcgagctgt tcgagacggg cgcgggtacg gctgagccgt tcgcggcacc
    13921 aggcgtcgcg agtggcgtac ggctcgggtg agaggaggaa ttcgtcggtg gccaccacgt
    13981 tgcgttctcg cgcgatagct atgccctcgc ctttgctcgt cgccccctcg ggcaagaagg
    14041 tgtagtcgta aagcaggaac atcggcacga tcgtggcggg gccgccgcgc tcggtccaca
    14101 ccgggaacgg atgttcgggg gtgaccacac ccatctcgtc gcacatgttg accaggtact
    14161 cgtagcgcgc cttgccgaag atctgcacgg gatcgcggtt ggtggtccac agctcgtggt
    14221 tgcccgggac ccagatcacc ttggcgaaac gccgccgcag caggtcaagg gcccaacgga
    14281 tctcgtcggt acgttcggcg acgtcgccgg cgacgatcaa ccaatcctcc ggcgacgacg
    14341 ggtgcagcga ctcggtgatc ggcttattgc ccaggtggcc ggtgtgcagg tcggaaacgg
    14401 cccacagcgt aggttgcgcg ccgttcgtcg attcatgctg cgatttttgc caagccacga
    14461 ccaaccagcc taatgactcg ctttcgaatg gccgttttcg cgcccgtcgc gggccgtgcc
    14521 cgggtgcctg ggggtgcgcg gtcggcggcc aagttagaac aagttcaggt ttcactgtgc
    14581 cgccgcgaac ccgacttgta cactccccgg caagggaccg ccgaggcatc agggggtgtc
    14641 gtgtccgcca acgtgcgtct gattggagcg cttggtgcgc tggtcaccac ggtcatcggg
    14701 ggcgcgatgg gcctgcgggg cgcgccgtcc gcgtcgaccg gagcccggcc gatcgagctg
    14761 cgatcgaccg ccgagccgat ggaaacgacc gcgccgacgg aaaccacgat gaagagcccg
    14821 atcgtggcga ccaccgatcc gcggcccttc gacgcgtgcg aggacatacc gttcgacgtg
    14881 atccagcggc tcgggttggc gttcacaccg cccgaacccg aggacgggct gcggtgccac
    14941 ttcgacgccg gcaactatca gatggccgtc gagcccattg tctggcgcac ctacgcgcaa
    15001 accctgcccg ccgacgccgt cgaaaccacg atccaggggc accgcgccgc acagtactgg
    15061 gttctcaagc cgacctatca caacagctat tggtattact cctgcatggt ggcgttcaag
    15121 accagctacg gggtgctaca gcaggcgctg ttcttctcga cggtctactc caaccccgac
    15181 gtcgactgca tgtccaccaa tctgcagcgg gccaacgacc tcgcgcccta ttacaagttt
    15241 taaggcaggt tttacggccc agcggccggg cccgcgtcga cgcgtgcggc cgccggccga
    15301 gcggggtctt agcctgggat cgtgactacg accgctaccc gacccgcgca gcgcaggctg
    15361 ggcgccctgc gacaagtcgg cggcaacgcc gtcggccgac tgctgggcct gcctccggcg
    15421 gccaccggct acaccgtgaa gcaggttcag gtgccgatgc gcgacggggt ccagctgctg
    15481 gccgaccact acgcgccgac caccgccgag ccccgcggca ccctgctggt ccgtgggccg
    15541 tatgggcgcg cgttcccgtt ctcgctggcc tacgccaggc tgtatgcggc ccgcggctac
    15601 cacgtggtgc tgcagagcgt gcgtggaacg ttcgggtcgg gcggggtgct cgaaccgatg
    15661 gtcaacgagg tgtccgacgg cgccgacacg gtcgattggc tgcgtgagca gcactggttc
    15721 accggccggt tcgccaccgt cggcgtgtcc tacctggggt tcacccagtg ggcgctgctg
    15781 gtcgatccgc caccggagct ggccgcggcc gtcgtcaccg tgggtccgca tgatttctac
    15841 gacgcggtgt ggggtaccgg caccttcgcg atcaacgact tcttgggctg gagccacgtg
    15901 gtttcgcaac aggaggaccc ggcgcgtatc cggggccgaa tccgccagct gggcgccccg
    15961 cgcagggtgg cgcgggcggt tgccgaggtg ccggtgggtg ccggtgcccg ggcgctgctg
    16021 ggcacgggcg caccgtggtt cgaatcgtgg gtcgaacaca gtgaccccga ggatccgttc
    16081 tggaaatcgc tgcgactcgg ccaggcgctg gaccgcgtgc aggtgcccgt gctgctgctc
    16141 agcggctggc aggacatctt cgtgcagcag acgttgcagc agtatgccca tctgcggggc
    16201 cggggcgtcg acgtcgcgct caccgtcgga ccctggacac acaccgagat gctcaccaaa
    16261 gggctggccg tcggcgccag ggacacactg gactggctgg acgcccacct gggcggcacg
    16321 gaggcgctgc gccgtcccag cagggtccgc gtcttcgtca ccgggctggg ctggcgcaac
    16381 ctacaagact ggccgccggc caccaccgag cgcgcgctgt acctgcggcc cggcggctat
    16441 ctcggtgaga ccgagccaac agacctgacc ggcgtggcac ccgcgaagtt ccgctcggac
    16501 cccaccgacc cgacacccac caccggcggt ccgctgctgt ccccggacgc cggttatggc
    16561 gacgacagca agctggcacg gcgcgcggac gtgctcgcct tcaccagcgg cgccctcgcc
    16621 caggatctga ccgtgtacgg aagccccgtc gtggaattgg cgcacacctc cgacaatccg
    16681 tgcgccgacc tgttcgtccg ggtgagccag gtcgacgcca agggccgttc ccgcaacgtc
    16741 agcgaagcct accggcgact gggaccggga acggagaccg tccgcatcga actcgacggg
    16801 atcgcgcacc gcttcccggc cgggacacgc atccgcctcc tgatcgccgg cagctggttc
    16861 ccccgttacg cgcgaaacct cggcaccggg gaaccggtgc tgaccggccg gcactcccag
    16921 ccggtcaccc acgtggtgca ctacggacgg tcccggctgc tgcttccggc cggccccgcc
    16981 gatccgtcag ccgaccgcgt cgcggacccg ggcagcgacg gcagctagcg cggcgtcgtc
    17041 gtgaaccggg tcgtgcgccg gccgcactga tacgaggtca gccggcgtca gcggcagctg
    17101 cacccagctc gtgcagcccg cgtactcggg ggtgcgagcg atcagcaccg gctcggccaa
    17161 cggaatcacc gacaccacca gcacggccag tttgtgcttg ggccggaagt cgagccggtc
    17221 ggcgcgcacc gactcggcgg tccagatgtg cagatcctcg atcgccggga gaccctctgg
    17281 ccggttaacc ggcaccgcgg caacaacttt cgccgcagcc cggacgagca ggcggtcgtc
    17341 ggtgctgtcc gtggccccgg cttccagcag gtctcgatgc tcgggtcgga cccgctgcgc
    17401 gtggctgtgg gcgaccgtgg ggaacaggaa gaactcccgg gccgccaact cgaaccgctt
    17461 ctcgccgatg ccgcccttgc gcagcagcac ccgttgccga ccggccagca gcccatgcac
    17521 caccgcggcc cactccttga gggcgggcat cgttgcggtc agggtcattt cgcgccggcc
    17581 agcctggcga cgacctcggg ccgccgcagc ggcggcacgg tcttgggcgg ctggcgtcgc
    17641 ggcgccaatg cccccagcaa tcgcgtcgtc gtctcggtga cctcggcgac ggccgcctcg
    17701 aatgcttcgg cgttgactgc cgaggggtgc gtgatgccgc tgaccttgcg cacatattgg
    17761 cgcgcggccg ccgcgatctc ctcgggtgtg gccgacggct gcaagccgcg cagttcggtg
    17821 atgttccggc acatgcctcc accataggca cgggcgcgtg tcgtctcatc cgccacatgg
    17881 gtctgtgccg acaagtgcgc tgctcgcgcg ctctggcgat aaccaatacc atccctccaa
    17941 ctggacgaaa gcagtcccca gcccaggagg aaccggatgc gtgctggccc gctcaccgtg
    18001 caggccctca ccgtgcgggc ccttggtggc ctgactctgg cggcggcatc ggcgatcgcg
    18061 gggcccgcgg cgcaagcggg cgcgcagccg cctccggggt ttcccaatct cgacactttc
    18121 gccgcggtcg cggcggacgg ctacgtcacg atgtctgacc cgggcgcctc tccacgggtc
    18181 agtttctccc ccacgtccac cctggtgtgc accttctatg gggggccggc gccgccgccg
    18241 caaccgtcgc aggacatcaa gtgcaacggt gacatgcccg gcatggacga tgttcccttc
    18301 cccggcggcg gccacaccaa acccggcgac tgcgtggtgg gcgccgtgaa cttcaaggga
    18361 cccggctacg agttgagccg catgtcctac ggcggctgcg acggcagttc gcccccattg
    18421 ccgtccggcg gcaaatcact gggtgcgggc cagaagttgt cgtacctgaa cgtgacttgc
    18481 gccgtcggag ccgacaatct cgtcgcctgc ctcgacgcca ccagcgggga ccacggcttc
    18541 gtcctgcagc ggtcagggag ctgggcgttt tagcgagcga tcagcccagg gcggcgcaca
    18601 gcgacgccac gacgtcctct atcgagccgg cggtggagta gccggcggcg agccggtggc
    18661 caccgccgcc gaacgtggag gcgaccgtcg ccaaatccat gtcggccttg gcccgcatcg
    18721 acaccgacca ctgctgcggg gcgatctcct tgaagaccgc cgccacctcg gcctgcgcgg
    18781 tggtccgcac gatgtcgacg atgctctcca cttcctcggg gcgagaatcg atccagtccc
    18841 gattctcgac aaccgcgtag accagcccgt ggccaccggc cgcctcccgc agcagctgcg
    18901 ccgagcccag cacgcgcgac aacagcggca accacccgaa cggatgggta tccatcagcg
    18961 tccggctgat ggcggcgttg tccacgccgg tgtcgaccag ccgagccgcc agccgcaggg
    19021 cgcgggcgct ggcccagcgg aacgatccgg tgtcggtcgt caggccggca tagatgcagt
    19081 gcgcgacctc gaggtcgatc ggcttgcccc aggtgtcgag cagttcggcg atcatcatcg
    19141 tggtggaatc cgccgacacg tcgatgaagt tcgcggtacc gaacatgtcg ttggaggcgt
    19201 ggtggtcgat gaccagcaac tgccggccgg gagcggccaa atcgctcaac tcccccagcc
    19261 gcttgaggct cgggacatcc acggtcacca ccagatcgac atcgcggcgc atcgcgtccg
    19321 gggtgaccag cagctcacag cccggcagcg aactcagcga ctccggcagt gtcgccgggg
    19381 aggcgaaact gacctccacg tccttgccgc accggtccag caccagggca agcgccagcc
    19441 ccgcgccgat ggtgtcggcg tcggggtgga catgagcgat caccgcgatc gtcgcggcgc
    19501 acgacagcaa ctcgacggca ccgagtgcgt cgactcgtgc ccccacgcac gaagtgccgg
    19561 ccagctcagt cttcggattg gtcgtcgtca ccggcatccc tagcgtcgag cccgtcgagt
    19621 gcccccgcct gcggttccgc gaacgtcccg ctgacccggt atggatcggc ctccccggcc
    19681 ggtttggcgc ccgagcgcac ccgcgccacg tcggcgtccg cggcgtgagc gcgcgccaac
    19741 aactcgtcca tccgatgcac gctgtccgac gtcgtgtccc gagtgaacgt cagcgtggga
    19801 gtgaagcgca cgccggtgcc ggcccccacc ctggtgcgca gcgttccttt tgcccgctcc
    19861 agcgcggccg cggcggcggc gtaatccggc tcgtcatcca gtgtgggccc catcacggtg
    19921 tagaacaccg tcgcgtcgtg caggtcggcg gtcaccttgg tgtcgacgat ggtcaccccg
    19981 tccagccccg gatccttgat ctcgaactcg attgccgagg cgacgatcgt gaaaatccgt
    20041 ttcgccagcc ggcgcgcccg ggcggggtcg gccatcaggc gcgctccttc tggaccagct
    20101 cgaaggactc gatgacgtcg ccttccttga tgtcggagta ggtcagagtg agaccgcact
    20161 cgtagccatc gcggacctcg gtcacgtcgt ccttctcccg tcgcagcgat gcgatggtga
    20221 tgttctcggc gaccacgatg ttgtcccgca acaaccgggc cttcccgttg cggcgaatca
    20281 caccggaggt gaccaggcaa ccggcgatca ggccgacctt cgaggagcgg aacagcgcgc
    20341 ggatttcggc gcgacccagc tggttctcct cgtagatcgg cttgagcagg ccgcgcaggg
    20401 ccttctcgat ctcgtcgatg gcctggtaga tcaccgagta gtagcggatg tccacgcctt
    20461 cgcgattggc cagctccgtc gccttgcctt cggcgcgcac gttgaagccg atgatcaccg
    20521 cgtccgaggc cgacgccagg ttgacgttgg tctcggtgat gccaccgacg ccgcggtcga
    20581 tgacgcgcaa ttccacctcg tcgtcgatct ggatccccag cagggcctct tccagggcct
    20641 cgacagtacc ggcgttgtcg cccttgagga tcaggttcag ctggctggtt tccttcagcg
    20701 ccgagtccag gtcctccagg ctgatccgct tgcgcgaacg cgccgccaga gcgttgcgct
    20761 tgcgggcgct gcgccggtcg gcgatctggc gggcgatgcg gtcctcgtcg acgaccagga
    20821 agttgtcgcc ggcgcccggc accgacgtga acccgatcac ctggaccgga cgcgacggca
    20881 gcgccgcctc gatgtcctcg ccgtgttcgt cgaccatgcg gcggacccgc ccgtaggcgt
    20941 cgccggcgac cacggagtca ccgacgtgca gcgttccgcg ttgaatcaac acggtggcta
    21001 cggggccgcg accgcggtcc aggtgcgctt cgatggccac accctgggcc tccatgtcgg
    21061 ggttggcccg caggtccagg gccgcgtcgg cggtcagcac gaccgcctcc agcagcgcct
    21121 cgatgttggt gccctgcttg gcggagatat cgacgaacat cgtttcgcca ccgaaatcct
    21181 ctgccaccaa accatattcg gtgagctgcg accggatctt ggtcgggtcg gcgccctcct
    21241 tatcgatctt gttgaccgcc accacgatcg gcacgtcagc tgcctgcgcg tggttgatgg
    21301 cctccaccgt ctgcggcatc acgccgtcgt cggccgcgac caccaggacg gcgatgtcgg
    21361 tggccttggc accgcgggca cgcatggcgg tgaacgcctc gtgacccggg gtgtcgatga
    21421 aggtgatcgg ccgctcggta ccgtccaggt cgacggcgac ctggtaggcg ccgatgtgct
    21481 gggtgatgcc gccggcctcg ccctcgcgga cgttggcctt acggatggtg tccagcagac
    21541 gggttttacc gtggtcgacg tgacccatca cggtcaccac cggcggacgg gtctgcaggt
    21601 cttcctcgcc gccctcgtcc tcgccgtagg tcaggtcgaa ggattccagc agctcgcggt
    21661 cttcgtcctc ggggctgacg acctggacgt tgtagttcat ctcgccgccc agcagctcga
    21721 gcgtctcgtc gccgaccgac tgggtagcgg tcaccatctc gccgaggttg aacagcgcct
    21781 gcaccagcgc cgccgggttg gcgtcgatct tttcggcgaa gtccgacaag gacgcgccgc
    21841 gcgccagccg gatcgtctcg ccgttgccgt gcggcaaccg cacgccgccg acgacgggcg
    21901 cctgcatcga gtcgtattcc tggcgtttct gccgcttgga cttgcggccg cgccggggcg
    21961 cacccccggg acggccgaac gcgccggcgg caccgccgcg ctgaccgggg cggccacccc
    22021 cgccgccacc ggggccaccg gggccaccac caccggggcc gccaccacca ggacggccgc
    22081 gaaagccgcc gggcgcagcg cctacgccgc ctccgcc
//
LOCUS       DASSGQ010000288         4555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1145325,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000288 DASSGQ010000000
VERSION     DASSGQ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145325"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFI55_12745"
     CDS             <1..250
                     /locus_tag="VFI55_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074937.1"
                     /translation="DVNFRPDLDDAVVRLGAPVTVGQVVAAYVDNAGAESDVVKAIEV
                     IDAADLGDEDAELIVGDALDHDLAWYANQELPFLLDLL"
     gene            500..1648
                     /locus_tag="VFI55_12750"
     CDS             500..1648
                     /locus_tag="VFI55_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HET7074938.1"
                     /translation="MSKKFESITGNVVATTRPVIAGADRHPGWHALRKIAARITTPLL
                     PDDYLHLANPLWSARELRGRIVEVRRETEDSATLVIKPGWGFSFDYQPGQYIGIGLLM
                     NGRWRWRSYSLTSSPAAQGPGSPRTVTITVKAMPEGFLSTHLVAGVEPGTIVRLAAPQ
                     GNFVLPDPAPPAILFLTAGSGITPVMSMLRTLVRRNQITDVTHLHSAPTESDVMFGAE
                     LAALAVDRPGYRLQVRETRRQGRLDLASLEQEVPDWRERQTWACGPEGMLAQAEKVWS
                     SAGISDRLHLERFAVSKAAPAGAGGTVTFVRSGRAVATDAATSLMDAGEGAGVQMPFG
                     CRMGICQSCVVSLVEGHVRDLRTGQEHDPGTRVQTCISAASGDCALDI"
     gene            1734..3029
                     /locus_tag="VFI55_12755"
     CDS             1734..3029
                     /locus_tag="VFI55_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET7074939.1"
                     /translation="MAITDVDVFAHLTDTDIENLAVELDAIRQDTEDSRGARDARYIR
                     RTIAAQRALEVTGRLLLAGSSRRSAWWAGTATLGVAKIVENMEIGHNVMHGQWDWMND
                     PEIHSSTWEWDMAGSSKHWRYTHNFVHHKYTNILGMDDDVGYGLLRVTRDQRWKRFNI
                     FNLLFNALLALGFEWGVGLQHVELGKMVMKRMDHDEARERTLEFIAKAGRQVVKDYVA
                     FPALTSLSPGATYRSTVTANWLANVIRNVWANAVIFCGHFPDGAEKFTKTDMIGETKG
                     QWYLRQMLGSANFEAGPALRFMSGNLCHQIEHHLYPDLPSNRLHEISVRVREVCDKYD
                     LPYTTGSFLVQYGKTWRTLAKLSLPDKYLLADADDAPETRSERMFAGLEPGFGGTDPT
                     TGRRRGLKTAIATVRGWRRDKRAGKQAEQTIGEADGLAA"
     gene            complement(3074..3940)
                     /locus_tag="VFI55_12760"
     CDS             complement(3074..3940)
                     /locus_tag="VFI55_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019928710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7074940.1"
                     /translation="MQTIDLAYVGRGIHEELVAHVADQEGYFEDEGIHVAVRDGVGWP
                     AERLRRGAVIGLGRALLSRLTDGIGWTALSVSTDHPLFWFLGNADVKSMADLRGRRLA
                     VHAPDNPPGTFARIVLRKHGLDPDRDLECVVRHPGDYQMDLRRLRDGSIDAAYVGSTL
                     SPEQVAQEEGFHVLAWVGDHFQIPTVGVVVDPVHIPLDSPALQALLRANQWALRTLTE
                     QPGRAVDYVNWFLDRLTRDEAQQHYDRYVGPYFSPGRRVDLDSAQEAIDAVAGELGVT
                     PIAAADFYQPTR"
     gene            complement(3991..>4555)
                     /locus_tag="VFI55_12765"
     CDS             complement(3991..>4555)
                     /locus_tag="VFI55_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7074941.1"
                     /translation="VTEAGEVAAMIDETERRWGRVDVLVHNALIPYDVTSFADLTWEQ
                     LGGKLDRELHAAFLVTKAVVPGMISRSYGRLVYLTTVLSRRPREGMITVGNAKAAMDQ
                     FVRYVALEVAPHGITANLVSPATVADTAANALLAAGEVHQLGATNPMGRLVRPDEVAK
                     TVAFLASEDSGFTTGHCLEVNGGLAMD"
BASE COUNT          792 a         1443 c         1549 g          771 t
ORIGIN      
        1 agacgtcaat ttccgtcccg acctcgacga cgccgtggtc cggctcggtg cgccggtcac
       61 ggttggccaa gtcgttgccg cttacgtcga caacgccggc gccgagtcgg acgttgtgaa
      121 ggcgattgag gtcatcgacg ccgctgactt gggcgatgag gacgcggaac tgattgtcgg
      181 cgacgctttg gaccacgatt tggcctggta tgcgaaccag gaactgccat tcctgctgga
      241 cctgctttag cctgccgacg atgcccggcg cgtagcgctg ggaggaggag gcgggcaatc
      301 gctgtagccc accgacgatg tccgctgacc tgggcgatcg tggatttagc cggtcagatg
      361 tgacggatgt ctggcgctcc agcgtggata cggtaccgta ggttacggca tcgtagcctt
      421 taaccagcgt ttctcagagg cgtggaatcg ccctgtggtg agcgggagct aagtgccgca
      481 tggtgagcag gagcttccta tgagcaagaa attcgaatca atcaccggca atgtcgtcgc
      541 caccacgcgt ccggtgatcg cgggcgccga taggcatccc gggtggcacg cgctgcgcaa
      601 gatagctgca cgcatcacga cgccgctgtt gcctgacgac tatctgcatc tggccaatcc
      661 gctgtggtcc gcgcgggaat tgcggggccg catcgtcgag gttcgccggg agaccgaaga
      721 ttccgcgacc ctggtgatca agccgggttg gggtttcagc ttcgactacc agccgggcca
      781 gtacatcggg atcgggctgc tgatgaacgg acgctggcgc tggcggtcgt attcgctgac
      841 gtcgagcccg gccgcccagg ggcccgggtc gccgcgcacg gtcaccatca ccgtcaaagc
      901 tatgcccgag ggctttctgt ccacgcacct cgtggccggc gtcgagccgg ggaccatcgt
      961 ccggctggcc gcgccccagg gcaacttcgt cctgcccgac ccggcaccgc cggcgatcct
     1021 gttcctgacc gccggctccg ggataacgcc ggtgatgtcg atgctgcgga ctctggtgcg
     1081 ccgcaaccaa atcaccgacg tcacgcatct gcattcggcg ccgaccgaat ccgacgtgat
     1141 gtttggcgcc gagttggctg cgctggcggt cgaccggccg ggttatcgct tgcaggtgcg
     1201 ggagacccgc agacagggtc ggctcgatct tgcttcgctc gaacaagagg tgcccgactg
     1261 gcgtgaacgc cagacctggg cgtgcggtcc ggagggcatg ctcgctcagg ctgaaaaggt
     1321 gtggtcgtcg gcgggcatca gcgaccggct gcacctggag cgattcgcgg tgtccaaggc
     1381 cgcacccgcc ggggccggcg gaacggtcac gttcgtccga agcgggcgcg ccgttgccac
     1441 cgatgccgcg acgtcattga tggacgccgg tgaaggcgcc ggcgtgcaga tgccgttcgg
     1501 ctgccggatg ggcatctgcc agtcctgcgt ggtcagcctg gtggaaggcc acgtccgtga
     1561 tctgcggacc ggccaggaac acgaccccgg aacccgggtt cagacctgca tatcggccgc
     1621 ctcgggcgat tgcgcgctcg acatctaatg attactcaca ggtaacctac ggattcgtag
     1681 gttacgatag cgtaagtaac tgaccggaca acgagcacag ggggatcaag acgatggcga
     1741 tcacagacgt cgacgtattc gcgcatctga cggataccga tatcgaaaac ctggccgtgg
     1801 agctcgacgc gatccgccag gacaccgaag actctcgcgg cgcgcgagac gcgcgctaca
     1861 tccgtcggac cattgcagcg cagcgtgcgc tggaggtaac cggccggctg ctgctcgccg
     1921 ggagttcacg gcgttcggcg tggtgggcag gcacggcgac cctgggcgtg gccaagatcg
     1981 tcgagaacat ggagatcggc cacaacgtca tgcacggcca gtgggactgg atgaacgacc
     2041 cggaaatcca ctcctcgacg tgggagtggg acatggccgg gtcgtccaag cactggcgct
     2101 acacccacaa cttcgtgcac cacaagtaca ccaacatcct cgggatggac gacgacgtcg
     2161 gctatggcct gctgcgcgtc acccgcgacc agcgctggaa gcgcttcaac atcttcaacc
     2221 tgctgttcaa cgctctgctc gcgctcggct tcgaatgggg cgtcggcttg cagcacgtcg
     2281 agctcggcaa gatggtgatg aagcggatgg accatgacga ggcccgggaa aggacgctgg
     2341 aattcatcgc caaggccggt cgccaggtgg ttaaggacta tgtcgcattc ccggccttga
     2401 cctcgctgtc gccgggcgcg acctacaggt ccaccgtgac ggcgaactgg ctggccaacg
     2461 tcatccgcaa tgtgtgggcc aacgccgtga tcttctgcgg gcacttcccc gacggcgccg
     2521 agaagttcac caaaaccgac atgatcggcg aaaccaaggg ccagtggtat ctgcgtcaga
     2581 tgctgggcag cgccaacttt gaggccggcc ccgcgctgcg gttcatgagc ggcaacctgt
     2641 gccaccagat cgagcaccac ctgtatccgg acctgccgag caatcggctg cacgagatct
     2701 cggtgcgggt gcgcgaggtc tgcgacaagt acgacttgcc gtacaccacg ggctcgttcc
     2761 tggtgcagta cggcaagacg tggcgcacgc tggcgaagct gtcgctgccg gacaagtacc
     2821 tgctcgccga cgccgatgac gcgccggaga cccgcagcga gcggatgttc gccggactgg
     2881 agccgggctt cggtggcacc gatccgacga cgggacgccg acgcggcctg aagacggcca
     2941 tcgccacggt gcggggctgg cggcgcgaca agcgcgccgg gaagcaggcc gagcagacga
     3001 tcggagaagc cgacggcctg gccgcttaag ggccgttaag gccttcaggc cgttatcttt
     3061 tcggccacgg cgctcaccgg gtgggctgat agaagtccgc ggcggcgatc ggggtgacac
     3121 cgagttcgcc ggcgaccgcg tcgatcgctt cctgtgcgct gtcgaggtcg acccggcggc
     3181 cggggctgaa gtagggcccg acgtagcggt cgtagtgttg ttgcgcttca tcgcgagtga
     3241 gtcggtcgag gaaccagttg acgtagtcga cggctcggcc gggttgttcg gtgagggtcc
     3301 tcagtgccca ttggttggcc cgcagcaggg cctgcagggc ggggctgtcc agcgggatgt
     3361 ggacggggtc gacgacgacg ccgacggttg ggatctggaa gtgatcgccg acccaggcca
     3421 gcacgtggaa cccttcttcc tgggcgacct gttcgggcga gagtgtgctg ccgacgtagg
     3481 cggcgtcgat cgagccgtcg cgcaggcgtc gcaggtccat ttggtagtcg cccggatgcc
     3541 gcaccacgca ttccaggtcg cggtcgggat ccaggccgtg cttacgcaag acgatgcggg
     3601 cgaaagtacc gggtgggtta tcgggagcgt ggacggccag gcgccgcccg cgaagatccg
     3661 ccatggactt cacgtccgcg ttgccgagaa accagaacag cgggtgatcg gtgctgaccg
     3721 acagcgccgt ccaaccgatc ccgtcggtca gccgcgacaa cagggcgcgg cccaggccga
     3781 tcaccgcgcc gcggcgcagc cgctccgccg gccagccgac cccgtcgcgg acggcgacgt
     3841 ggatgccctc atcctcgaag tagccctcct ggtcggcaac atgggcgacc aactcctcgt
     3901 ggataccacg cccgacgtag gccagatcga tcgtctgcac ggccaaaacc cctttccgat
     3961 ctgctgactt cgcttgcggc ggaacctgtt tcagtccatc gcaaggccgc cgttgacctc
     4021 caggcaatgg ccggtggtga agcccgaatc ctcgctcgcc aggaaagcca ccgtcttggc
     4081 cacctcatcg gggcgcacga ggcgccccat gggattggtt gcgcccagtt gatgcacctc
     4141 tccggccgcc aacagcgcgt tcgccgccgt atcggccacg gtggcgggcg aaacgaggtt
     4201 cgcggtaatc ccgtgtggcg ccacctcgag cgcgacgtac cggacgaact gatccatggc
     4261 cgccttggcg ttgcccaccg tgatcatgcc ttcgcggggc cggcgggaca gcacggtggt
     4321 gagatagacc agtcggccgt agctgcggga aatcataccc ggcacaacgg ctttcgtgac
     4381 caggaaggcg gcatgcagct ctcggtccag cttgccgccg agttgctccc aggttaggtc
     4441 ggcgaaggag gtgacgtcgt aagggatcag tgcgttgtgc acgagcacgt cgacccggcc
     4501 ccaccgccgt tcggtttcgt cgatcatcgc ggcgacctca cctgcttcgg tgaca
//
LOCUS       DASSGQ010000289         6940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1296560,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000289 DASSGQ010000000
VERSION     DASSGQ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6940
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296560"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1186
                     /locus_tag="VFI55_12770"
     CDS             194..1186
                     /locus_tag="VFI55_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014982232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7074942.1"
                     /translation="MIVAVLALDRVMGFELMIPGQVFGMANLAAAERGTGGTDGAAPS
                     PPGYEVRVCGQRPSISTSADWGLVEIHTPHGLDTLVDADLVVVPGTHNFLEEPDPQAI
                     SALQAAADNGARVASMCVGAFTLAAAGLLNGRRATTHWQWAGELARRYPDVDVDPRVL
                     FVDEGPVVTSAGVASGLDLCLHLVRQHSGPDLAGRTARRVVIPAWRDGGQAQYIEHAG
                     PADPGHPLQPTIAWMEENAHAALDLQAIADHASVSVRTLNRQFREHFGATPLGLLVRM
                     RVDRARRLLESTGLSVDRVAEQSGFGSCASLRFHFHRTVGVSPQKYRQNYAAEH"
     gene            1250..1858
                     /locus_tag="VFI55_12775"
     CDS             1250..1858
                     /locus_tag="VFI55_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7074943.1"
                     /translation="MTSGRDRLLAAALKLFAAKGYAATSVADIQRASGLAPGSGALYK
                     HFGSKRELLEAAVAYRIDSIVAAREQYDSGEPRSVEQAVRTAGKLIWSNLKDSEDLLR
                     VMLREPDELGDLDEKTWQIITDNAYQRFADELVASNRSGRTHIPDPAATAAAAIASLS
                     YAATLQALTGRLPGNIEEDRYFEAWVNQTLSVLAQYRSPKKG"
     gene            1885..3096
                     /locus_tag="VFI55_12780"
     CDS             1885..3096
                     /locus_tag="VFI55_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7074944.1"
                     /translation="MTFSLQLSDDVIEVRDWVHQFAAEVIRPAAAEWDEREETPWPVI
                     QEAAKVGLYSPDFFAQQAAEPTGLGMLTAFEEMFWGDAGIALSIMGTGLAAAALAGNG
                     TPEQLGRWLPDMFGTPGDPKLGAFCSSEPDAGSDVGAIRTRAQYDEAAGEWVLNGTKT
                     WATNGGIANVHIVVASVCPELGTRGQATFVIGPDTKGLSQGQKFKKHGIRASHTAEVV
                     LDNVRLPEDQILGGREKFEARIARISSGASVRGQAAMKTFERTRPTVGAMAVGVARAA
                     YEYALEYACQREQFGRKIGEFQAVAFKLADMKSRIDAARLLVWRAGWMARNNQRFDSA
                     EGSMAKLVASETAVYVTDEAIQILGGNGYTRDYPVERMHRDAKIFTIFEGTSEIQRLV
                     ISRALTGLPIR"
     gene            complement(3140..4030)
                     /locus_tag="VFI55_12785"
     CDS             complement(3140..4030)
                     /locus_tag="VFI55_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016692282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET7074945.1"
                     /translation="MRIANHDGRAVLVIGESGSEKGPEKGIDIATASGGRFGPDLTGV
                     YPEWDELCNWATSYDSAGASSSPIDRTLLGAPSPCPRQVFALGLNYGEHAAEAGFEAP
                     TALPPVFAKYVSSFSGPDTTVVIPEGGNVDWEVELVAIIGRQAANISEAAAWDHVAGL
                     TVGQDVSERITQLRGPAPQFGLGKSYPGFSPQGPWLVTPNELANPDDLELGCAIDGEE
                     VQNSRTSSMIYPVSKLVASLSANVTLYPGDVIFTGTPSGVGIGRKPQRFLRPGEHLRS
                     WISGIGELNQTFVGAAAATS"
     gene            4189..5406
                     /locus_tag="VFI55_12790"
     CDS             4189..5406
                     /locus_tag="VFI55_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7074946.1"
                     /translation="MNTEIRVLDNEDDLIAAANVFRAAMVGFPPLSNLAPGQITRLLE
                     PGRTIGAFVKGQLVGTADAVTSGLTLPGGAIVSHAAVTHIGVLPSFTRKGIATDLIGH
                     QLDDIAARGEVVATLRASEATIYERYGYGVASSSQTVEVRTARATLRPDIGAGGPVRL
                     LDAAEAWDVLPRIYAGNRPSRPGTIDRPQVWWQGLRMRSEFSSGPSYVAVHGEPGCES
                     GFVRYRPVDTGEWFISDLRTIAVEDFFAPTTEAYLGLLRFLLGLDLIDRVMFWMLPVD
                     DPLPWLLVDRRAIKVTAVHDETWLRIIDVQSALAARRYEGDAGVTIAVTDPMLPSNSA
                     SFTIAADGVEPTQRRAELHIGVDGLGAVLLGGATWRGLAVAGLVRADDPAALSVADRL
                     FAVLEAPHAGFFF"
     gene            complement(5468..6166)
                     /locus_tag="VFI55_12795"
     CDS             complement(5468..6166)
                     /locus_tag="VFI55_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7074947.1"
                     /translation="MVWQLAAATGLFAASQLPPPADVVAALGELIRRGELWIDLEASL
                     QRVLLGYLAGAAGALALGSLVGLSATVRRLLGPTVAAFRTVPSLAWVPLLLLWFGIDE
                     TPKVLLVAIGAFFPIYTTTASALSHVDAHLLEVGRAYGRHGASLLTAVMLPAAAPELV
                     NGLRLGLANAWLFLVAAELIASSKGLGFLLIDSQNTGRTDVMLLAIVLLAGLGKLSDA
                     VLGTLEHWLVRRRG"
     gene            complement(6238..>6940)
                     /locus_tag="VFI55_12800"
     CDS             complement(6238..>6940)
                     /locus_tag="VFI55_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042918 - alkanesulfonate transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7074948.1"
                     /translation="VALVARANGTPIKIVDVYSKPEWTALVVGKDSPVNSVADLKGKK
                     IAVTKGTDPYFFLLQSLARAGLSPSDVEIVNLQHADGKTALERGDVDAWSGLDPFMAQ
                     TVQQQGSRLLYRNPDFNSYGVLNVREDVIAAHPDFVQSVVNSYEEARKWAKAHPDELA
                     ALLASQAKVTASVAQEELHRTALDIQPAPGDPQRAVLTNILPIAVADSDIKSDEAGRN
                     ALSSIIEPKYAQQAS"
BASE COUNT         1189 a         2308 c         2294 g         1149 t
ORIGIN      
        1 ccggtccgac gtcgagcacg aattgtgttg ctgcgcgggc aatgtcggaa tctggaaggt
       61 tcagcgggct cgccggccgg cttgcgttgg cggtcatcgg gatcctgagc tgcgtgacat
      121 accgtcacgc tgccgcagtg gcgccgggaa cgccagtggc cagattgcct aatatcgcta
      181 gaatctagcc acgatgatcg ttgcggtgct ggcgctcgac cgggtcatgg gattcgagct
      241 gatgattccc ggccaggtct tcggcatggc caacctggca gccgccgagc ggggaaccgg
      301 cggcaccgac ggagcggctc cctcgccacc gggctacgag gtccgggtgt gcgggcaacg
      361 cccgtcgatc agcacctcgg ccgactgggg tctggtcgag attcacacgc cccacggtct
      421 cgacaccctc gtggacgcgg accttgtggt ggtgccggga actcacaact tcctggaaga
      481 gcccgatccg caggcgatct cggcgctgca agccgctgcc gacaacggtg ctcgcgtcgc
      541 atcgatgtgc gtcggggcct tcacgctggc ggccgcaggc ctgctgaacg gcaggcgagc
      601 caccacgcat tggcaatggg ccggcgagtt ggcgcggcgc tatcccgacg tcgacgtcga
      661 cccccgcgtg ttgttcgtcg acgaaggccc cgttgtgacg tcggcgggcg tcgcatcggg
      721 cctggacttg tgcctgcatc tggtccgcca gcactccggc cccgacctgg ccggccgcac
      781 cgcgaggcgg gtggtgatac ccgcgtggcg cgacggcggc caggcgcagt acatcgaaca
      841 cgcgggaccc gccgaccctg gccatcccct gcaaccgacg atcgcttgga tggaagagaa
      901 tgcgcacgcc gcgctcgacc tgcaggccat cgcggaccac gcatcggtga gtgtgcgaac
      961 gctcaatcgg caattccgcg agcatttcgg cgccaccccg ttgggattgc tcgtacgaat
     1021 gcgcgtcgac cgcgcaagac ggctgttgga atccaccggc ctgagcgtgg atcgcgtcgc
     1081 cgagcagtcc gggtttggtt cctgcgcgtc cctgcggttt cacttccacc gcacggtggg
     1141 ggtgtcgccg caaaaatacc ggcagaacta cgccgcggag cactgacgca cccacgcgta
     1201 agccgcggcg atttgcccaa ccgttggtga gtgagtactc tctcactttg tgacgtcggg
     1261 acgcgatcgg ctgctggccg ccgcgctgaa gctgttcgcc gccaaagggt acgcggcgac
     1321 gtcggtggcc gacatccagc gggcgtccgg cctcgcgccg gggtccggcg cgctctacaa
     1381 gcacttcggc tccaagcgcg agctgttgga ggccgccgtc gcgtaccgga tcgacagcat
     1441 cgtggccgcg cgagaacagt acgactcggg ggagccgcgc agcgtcgagc aggcggtgcg
     1501 caccgcagga aagctgatct ggagcaacct caaagacagc gaggatttgc tcagggtcat
     1561 gttgcgcgaa cccgacgagc tcggcgacct cgacgagaag acctggcaga tcatcaccga
     1621 caacgcctat caacggttcg ccgacgaact ggtcgcctcc aatcgttccg gccgcacgca
     1681 tatacccgac cccgcggcga ccgcggccgc ggcaatcgcg tcgttgtcct acgccgcgac
     1741 gctgcaggcg ctgaccggcc gcttgcccgg caacatcgag gaagaccgtt acttcgaggc
     1801 gtgggtcaac cagacgctca gcgttctggc gcaatatcga agcccgaaaa agggctgaca
     1861 ccaaatgatt tcaggagcaa tgccgtgaca ttctcacttc aactgagcga tgacgtgatc
     1921 gaagtccgcg actgggtgca ccagttcgcg gcggaggtca tccgcccggc cgccgccgaa
     1981 tgggacgaac gcgaggaaac tccgtggccg gtgatccagg aggccgcgaa ggtggggctt
     2041 tactcccccg acttcttcgc gcaacaggcg gccgagccga cggggctggg catgctcacc
     2101 gccttcgagg agatgttctg gggcgacgcc ggcattgcgc tttccatcat gggcaccggg
     2161 ctggccgcgg cggcgttggc gggcaacgga actcccgaac agttaggccg ctggcttccc
     2221 gacatgttcg gtacgcccgg cgatcccaaa ctcggtgcgt tctgctcctc cgaacccgac
     2281 gcgggttctg acgtcggagc catccgcact cgtgcgcaat acgacgaggc cgcgggcgaa
     2341 tgggttctca acggcaccaa gacctgggct accaacggcg gtatcgccaa cgtgcacatt
     2401 gtggtcgcgt cggtctgccc cgaactcggc acccgtggcc aggccacctt cgtcatcggg
     2461 ccggacacca agggcctgtc tcagggccag aagttcaaga agcacggcat ccgcgcgtcg
     2521 cacaccgccg aggtggtcct cgacaacgtc cgactgccgg aggaccagat cctcggcgga
     2581 cgggagaagt tcgaagcccg catcgcccgg atcagttccg gtgcgtcggt gcgcgggcag
     2641 gcggcgatga agactttcga gcgcacccgg cccaccgtcg gggcgatggc cgtcggagtg
     2701 gcacgggccg cctacgaata cgccctcgaa tacgcctgcc agcgcgagca attcggccgc
     2761 aagatcggtg aattccaagc cgtggcattc aagctggcgg acatgaaaag ccggatcgac
     2821 gccgctcgtc tgctggtgtg gcgagcgggc tggatggccc gcaacaacca acggttcgac
     2881 tcggcggagg gctcgatggc caagttggtc gcgagcgaga cggccgtcta tgtcaccgac
     2941 gaggccatcc agatcctggg cggcaacggg tacacccgtg actaccccgt ggagcggatg
     3001 caccgcgacg caaagatctt caccatcttc gaagggacca gcgagatcca gcgcctggtg
     3061 atttcgcggg ccctgacggg cctgccgatc cgctagccgg ggatcccggc cactccccga
     3121 tttttgaggc gatgcggcct cacgacgtgg cggcagccgc cccgacgaaa gtttgattca
     3181 gttctccgat tccgctgatc cagctgcgca gatgttcgcc cgggcggagg aatcgttggg
     3241 gcttgcgtcc aattccgaca ccggatgggg tgcccgtgaa gatgacgtcg ccggggtaaa
     3301 gcgtgacgtt ggcggacaac gacgccacca gcttggagac ggggtagatc atggagctgg
     3361 tgcggctgtt ctgcacctcc tcgccgtcga tcgcgcagcc cagttcgagg tcgtcgggat
     3421 tggccagctc gttcggcgtg acaagccatg gaccctgtgg agaaaagccc ggataggatt
     3481 tgccgagccc gaattgtggt gcaggaccac gcaattgggt gatccgctcg gagacgtcct
     3541 ggcccaccgt caggcccgcg acatgatccc aggccgcggc ctcgctaatg ttggcggcct
     3601 ggcgtccgat gatcgcgacc agttcgacct cccagtccac attcccgccc tcggggatca
     3661 ccacggtcgt gtcgggcccg gagaagctgg acacatactt ggcgaatacc ggcggcagcg
     3721 cggtgggcgc ctcgaagccc gcttcggcgg cgtgctcgcc atagttgagt ccgagtgcga
     3781 acacctggcg cgggcacggg gacggcgcgc ccaacagcgt gcggtcgatc ggggagctgc
     3841 tggcgcccgc gctgtcgtaa cttgttgccc agttgcacag ttcgtcccac tcggggtaaa
     3901 caccggtgag gtcgggaccg aatcggccgc cggatgccgt cgcgatgtcg atacctttct
     3961 cgggaccctt ttcgctgccg ctttccccga tcacgaggac ggcacgacca tcgtggttgg
     4021 cgatgcgcat cagcactcac ttcctttcag tggttgcgcg ggtcattaga gcactggtca
     4081 gaccagcgag ttggggtaac gcgaacggca cgcgatcaat tctggcgtcg gcccatcgtg
     4141 tgatcaaccg tccgacggcg ggtcaggggc accagacatg atgtttggat gaacaccgag
     4201 atccgcgttc tcgacaacga ggacgacctc atcgccgcgg ctaacgtgtt ccgcgccgcc
     4261 atggtcgggt tcccgccgtt gtccaacctg gcgcccggcc agatcacgag gctgctcgaa
     4321 cccggccgta ccatcggtgc attcgtgaaa ggtcagctcg tcggcaccgc cgacgcggtg
     4381 acgagcgggc tgacgcttcc cggcggggcg atcgtcagcc atgcggccgt gacacacatc
     4441 ggggtgctgc cgtcgttcac ccgaaaaggc atcgccaccg acctgatcgg tcatcagctg
     4501 gatgacatcg ccgcacgcgg cgaggtggtc gcgacgttgc gggcgtcaga agcgacgatc
     4561 tacgagcgct acggctatgg cgtggcgagt tcgtcgcaga ccgtggaggt ccggaccgcg
     4621 cgcgcgacgc tacgccccga tatcggggcc ggtggtccgg tgcgactgct ggacgcggcc
     4681 gaagcgtggg acgtcctgcc tcgcatctac gccggcaatc gcccgtcccg tccgggcacc
     4741 atcgatcgtc ctcaggtgtg gtggcagggt ctgcggatgc gcagcgaatt ctcctcgggg
     4801 ccttcatatg tcgcggtgca cggtgagccg ggctgcgagt cgggattcgt ccgctaccgt
     4861 cccgttgaca ccggggagtg gttcatcagc gacctgcgca ccatcgccgt cgaggacttc
     4921 ttcgcaccca ccacggaggc ctacctcggg ctgctgcggt tcctgctcgg gctcgatctc
     4981 atcgaccgcg tcatgttctg gatgctgccc gtcgacgatc cgctgccctg gctgctcgtg
     5041 gatcggcggg ccattaaggt gaccgcggtg cacgacgaaa cgtggctccg catcatcgac
     5101 gtccagagcg cgcttgcggc acgccggtac gagggggacg cgggcgtcac catcgcggtc
     5161 accgacccga tgctgccgtc caattcggcc agcttcacca tcgccgccga cggcgtggaa
     5221 ccgacgcagc gccgcgccga actccacatc ggtgtcgacg ggctgggcgc tgtgctgctc
     5281 ggcggcgcga cctggcgcgg cctcgccgtt gccgggttgg tccgggccga cgatccggcg
     5341 gcgctcagcg tggccgatcg gctgttcgcg gtgctcgagg caccacacgc gggattcttc
     5401 ttctaacgcc agggacgcga tttccaacgc cagggacgcg atcgagttcg gcccgccgcc
     5461 cgtcgagcta gccgcgccgc cgcaccagcc aatgttcaag ggtgcccagc acggcgtcgc
     5521 tgagtttgcc caatccggcg agcagaacaa tggccagcag catcacatcg gtgcgacccg
     5581 tgttctggct gtcgatcagc agaaatccga gacccttcga cgacgcgatc agttcggctg
     5641 cgaccaggaa cagccacgca ttcgccaagc ccagcctcag cccgttcacc agctccggag
     5701 cggcggcggg cagcatcacc gccgtcaaca gcgaggcgcc gtgtcggccg taggcgcgcc
     5761 ccacctccaa caggtgtgcg tcgacatgcg acagcgccga tgccgtcgtc gtgtagatcg
     5821 ggaagaaggc accgatggcg acgagcaaca ccttgggcgt ctcgtcgatt ccaaaccata
     5881 agagcagcaa cggcacccac gccaacgagg gaaccgtgcg aaaggccgcc accgtcggcc
     5941 ccagcagcct gcgcacggtc gccgacagac cgacaaggga gcccagcgcg agcgcaccgg
     6001 ccgcacccgc caggtaaccg aggagaaccc gctgcaggct cgcttccagg tcgatccaca
     6061 gttcgccgcg acggatcagt tcgcccagcg cggccaccac atcggcgggc ggcggcaatt
     6121 gactggccgc gaacaatccc gtcgctgcgg ccagttgcca gaccaccagc agcagtaccg
     6181 ggacaaccag tccagtcagc gccggcggca gtttcgccgg ccgcgcggcg cacaacctca
     6241 agaagcttgc tgggcgtact tcggctcgat gatcgaactc agcgcgttgc ggccggcttc
     6301 gtcggatttg atgtcggagt ccgccacggc gatcggcagg atgttggtca gtaccgcgcg
     6361 ctgggggtcg ccgggtgctg gttggatgtc caatgccgtc cgatgcagtt cctcctgcgc
     6421 cacgctcgcg gtcaccttcg cctgcgaagc cagcaaggcc gccaactcgt cggggtgcgc
     6481 cttcgcccat ttgcgggcct cctcatagct gttgaccacc gactgcacga aatccggatg
     6541 tgcggcgatg acgtcctcgc gcacgttcag cacgccgtaa gagttgaaat ccgggttgcg
     6601 gtacagcaac cgcgaacctt gttgctgaac cgtctgcgcc atgaaggggt ccaacccgga
     6661 ccaggcgtcg acgtcgccgc gctccagggc cgtcttaccg tcggcgtgct gcaggttgac
     6721 gatttccaca tcgctgggcg aaagtcccgc tctggcaagc gattgcaaaa ggaagaagta
     6781 gggatcggtg cccttggtga ccgcaatctt cttgcccttc aggtcggcga ccgagttgac
     6841 cggcgaatcc ttgcccacga ccaaagccgt ccattccggc ttgctgtaga cgtcgacgat
     6901 cttgatcggc gtgccgttgg cccgggcgac cagcgcgacc
//
LOCUS       DASSGQ010000290        11725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_841746,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000290 DASSGQ010000000
VERSION     DASSGQ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11725
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841746"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VFI55_12805"
     CDS             <1..861
                     /locus_tag="VFI55_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7074949.1"
                     /translation="REQVVTMNSVATALGAVAAFLGADFMLVPRFFFGASDRGASAII
                     FITAIPVSIALLLSFRFGSRVLGPDDTKRAIHGSVVYAVITGWLHGARTVLQSPTVAA
                     TLSGLAAHRAVMGINSLVMLVLLHHMPAPPGGGLGIALLFFAASGLGAFLANVLTPSV
                     VRRFGRYATANGALAAAALIQIPGVALRLPVMAVCGFLLGVAGQMVKLCADSAVQIDV
                     DDALRGHVFAIQDALFWVSFIVSITVAAMLIPDDGHAPALVLFGSVLYLIGLAVHSIV
                     GRRGQPVNNR"
     gene            905..1678
                     /locus_tag="VFI55_12810"
     CDS             905..1678
                     /locus_tag="VFI55_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03084 family metal-binding protein"
                     /protein_id="HET7074950.1"
                     /translation="MAGPGPIVADLRAECDDLDALVAPLPPERWADPTPALGWTIAHQ
                     IAHLLWTDEVSLTAVTDEAGFAEVQKAAQANPEGFVDAAAEELAILPPADLLTDWRVT
                     RERLHEALVRVPDGRKLPWFGPPMSATSMATARLMETWAHGLDVADAVGVTRPATERL
                     RSIAHLGVRTRDYAFVINNLAPPTEPFLVELRGPGGDAWSWGPTDAAQRVTGSAEDFC
                     ALVTQRRPLSALDITAEGPDAQRWLQIAQAFAGPPGPGR"
     gene            complement(1711..3000)
                     /locus_tag="VFI55_12815"
     CDS             complement(1711..3000)
                     /locus_tag="VFI55_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019737174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5631 domain-containing protein"
                     /protein_id="HET7074951.1"
                     /translation="MAIFGRMSARQRLRRATRESLAIPTFNAPVDCTPWVTGGLWPAE
                     LSTCTAETATLAEYLKADLQRITTSANDELKIIKRAGMPDSVRLAAEVGVIEEARAQA
                     VRRVESTLRQLHAAKAEPQAEHPRPQGAESDLESTQVNPAVSDEEPAADASMDGAPES
                     PEVFESEGDDAPVVGVVAGPEAGERDSPEAPEGRHRAADDDGADKAITPATPEVIAPT
                     AASESDDERLQRLLAFVVQQEPRLNWGVGDRADGTTILVTDLAHGWIPPGITLPAGVR
                     LLEPERRSGKVSALVGETRRTLTYAPGDSLGRLDDFAPTRASAQPRELPAVDDLGWEL
                     SQATHWRDGLPRMVHTLARAAAAGTGVVEEEVDLLRVHLDTARYQLVIQYPNVDPALL
                     LNCLLLAATEGAATADPISAHYHLAWFRKLDASAASP"
     gene            3083..3226
                     /locus_tag="VFI55_12820"
     CDS             3083..3226
                     /locus_tag="VFI55_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074952.1"
                     /translation="MADSALQQQLDEVRALLARARELFGPNPIEPPTDIAPNPESAKT
                     WLL"
     gene            complement(3252..3563)
                     /locus_tag="VFI55_12825"
     CDS             complement(3252..3563)
                     /locus_tag="VFI55_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2694 family protein"
                     /protein_id="HET7074953.1"
                     /translation="MTDANPAFDTVHPSGHILVRSCRGGFMHSVALSEEAMETDAETL
                     AQGILLTADVSCLKALLEVRDEIVASGHTPSMEVPTPHDLDAAIEKLLAHKLRRRHSA
                     R"
     gene            complement(3560..3877)
                     /locus_tag="VFI55_12830"
     CDS             complement(3560..3877)
                     /locus_tag="VFI55_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESX-1 secretion-associated protein"
                     /protein_id="HET7074954.1"
                     /translation="MADSIRAVPARLREAAAHHQETSDYLRTIPSSHAAIQESLDSLG
                     PIFSELRAAGRELLELRRQCYEQQADDHADIAEKLNVSAAMWEQHEDDAARNFGGIVD
                     GGR"
     gene            complement(3930..5372)
                     /locus_tag="VFI55_12835"
     CDS             complement(3930..5372)
                     /locus_tag="VFI55_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4226 domain-containing protein"
                     /protein_id="HET7074955.1"
                     /translation="MSTYEELLATVKLVRDSTGDPNAWQAGLAPNEVAAVITPMTPPE
                     QLDAILHKIRQQHSDLFGAGTPGGPPAWRPPDAPGASSDRAEGAAADAMADAEAALAH
                     QNSASSQLDMQVISAILNAHLKAVDGREALTKLQRETEDAVRTRSDLDTPAGARDFQR
                     FLIGKLKDIRTVVLDANLDDTSKSALMAAWTSLYDASKSESTIPVAGTTAQQPVDDLD
                     TGWDPLLDSLTADDPGWVSGDSSGHGSTEAASAPATPPAPVMPAMPNLGVGPLPGLGA
                     APGSMAGWGEPGGFPLTGFGDGNRTDPALLAADDQRPPGSHDPEPSDPPASDETGTDR
                     HGEDASADTPESSPAGPTTLTLPDGETVTAATPQLAAAIKAAVGGAPIADAFQQQGIT
                     IPPPGTAVTNPIDPRQVLPGDIGVFTDRHALAVGNAKAVLDGQIQHIATVSGPNFLGW
                     EHPPAATAPASAPAKPGTPTPTRPAATSTT"
     gene            complement(5508..5870)
                     /locus_tag="VFI55_12840"
     CDS             complement(5508..5870)
                     /locus_tag="VFI55_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4226 domain-containing protein"
                     /protein_id="HET7074956.1"
                     /translation="MSEQAGLSVDAIQARQSALARQHSTVADADRALAEVLASVHAAS
                     RESIRRLDAIADQIHRAAIDRADPALDTPMGAREFQKFLAAKQREIAAVVADAHELDR
                     AKRVVLDGLRAQYAVSAG"
     gene            complement(5919..6746)
                     /locus_tag="VFI55_12845"
     CDS             complement(5919..6746)
                     /locus_tag="VFI55_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HET7074957.1"
                     /translation="MIDSDVEVLRRAHKLFAGSSWHPTLEAGTAPYRGLLQRTAGLNH
                     GLGRGGYQLAADDSRQRLLSAARTDAEAAAVIAGAHQDRVEAGELTKGVLDEARADAT
                     ATPMTPMAQREAVRRQVTRLRTQRAHVLSARLRAQQHSAALRALRYRMMHHRGLGLRL
                     PSSNGRAAIAVRAALSRVGRPYVWGATGPDEFDCSGLVQWSYAQAGVHLDRTTYQQIN
                     DGIPVSRSQVRPGDLVFPHAGHVQLAIGTNLVIEAPYSGASVRISPLGNNVAVRRPL"
     gene            complement(6743..7531)
                     /locus_tag="VFI55_12850"
     CDS             complement(6743..7531)
                     /locus_tag="VFI55_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7074958.1"
                     /translation="MSRESAGAAIRALRESRDWSLADLAAATGVSIMGLSFLERGARK
                     PHKSTVQKIENGLGLPPGTYSRLLVAADPDAELARVMAARPPDDRPAAPGGAVVVDRH
                     SDTDVLEGYAEAQLDALKSVIDRLPATTSNEYETYILSVIAQCVKAEMLAASSWRVAV
                     NAGADSTGRLMEHLRALEATRQDLLKRMPTSLSARFDRACVQSSLPEPIIAALVGVAN
                     DDMWHIRNRGVIPPAALPRVRAFTEAVEATSRGEADQDSGEGEQ"
     gene            7672..8088
                     /locus_tag="VFI55_12855"
     CDS             7672..8088
                     /locus_tag="VFI55_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HET7074959.1"
                     /translation="MGNPCAANPELWFGYPDDDGGDGAAKARAYERSATEARIQCLRR
                     CPLAEQRRCAQHAIAHREEYGVWAGVKLPGGQYRKREQLARAHDILRRIAAGEINSRQ
                     LPENAALLTRHEHDAVAVTAVVLHLPTAQVGPRSAA"
     gene            8186..9568
                     /locus_tag="VFI55_12860"
     CDS             8186..9568
                     /locus_tag="VFI55_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7074960.1"
                     /translation="MTFDLTPTAAQHDLARRTHEFAEEVVRPVALEYDQRQEFPWPVL
                     EEAARRGFYSPLFYRDLIGDPTGISLPMFMEELFWGCAGIGLAIVMPALALSAIGQAA
                     SPEQMLAWAPECFGTPGDLKLAALAISEPEGGSDVRNLRTRARRDGDDWIIDGHKMWI
                     GNGGIANVHVVNAVVDEELGHRGQALFVVPGGIPGLKMVRKLDKLGCRASHTAELLFD
                     GVRVPAANLLGGEEKLEHKLAKAREAVAGGQRSGSATLGTFEQTRPMVAAQAIGIARA
                     ALEYATQYATEREAFGGPIIDNQGIAFPLADLATEIDAARLLTWRASWMAASGVPFER
                     GEGSMSKLAASEVAVKATERAIQTMGGWGYITDHPVEKWYRDAKLYTIFEGTSEIQRM
                     VVSNALGAAVGTPPLHVVIEPTGGPLNRIFGRGTPLRSRAADVALSMQDRVPPPVMRL
                     AMKLLRPPGR"
     gene            9629..10600
                     /locus_tag="VFI55_12865"
     CDS             9629..10600
                     /locus_tag="VFI55_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015353907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET7074961.1"
                     /translation="MSNLEAAPAEVACTASRVTGIEVLQPREVPLGGPRAMHVQRTLP
                     QQQRSLIGAWCFIDHYGPASARMDVPPHPHTGLQTVSWLFSGEVEHRDSAGVHAMVRP
                     SELNLMTAGAGICHSEVSIESNTALHGVQLWVALPDSDRDTGRDFAHYEPPPVSLGGA
                     VARVFLGELAGHRSPVHTFTPLLGAQIDLDVNAGLDIDLDPAFEHGVLCDAGPVEMGG
                     VALAAAELGYQSPGSSSLHLRNVGDRPARVVLLGGTPFTEQLVMWWNFVGRSHDDIVG
                     YRQLWEDADDRFGAVHGYRGSVARLPAPPLPITRLLPRPIPQRKGHT"
     gene            10597..10938
                     /locus_tag="VFI55_12870"
     CDS             10597..10938
                     /locus_tag="VFI55_12870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7074962.1"
                     /translation="MTVDKAGAQTTVTEEEDRYTIAVDGKLVGLADFADRGDQRVFYH
                     TEIDPAYGGRGLGTILVEGALNATRDEGKRVVPVCSMFVTTLKKHPEFDDITDPVTGD
                     IQQWLHSQPAG"
     gene            complement(10995..11261)
                     /locus_tag="VFI55_12875"
     CDS             complement(10995..11261)
                     /locus_tag="VFI55_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011742436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HET7074963.1"
                     /translation="MSSERVPGGAVHKLPTDMREALIANSVALAAWKDIPPLARNEFI
                     CWVEDAKQDVTRTRRIRRTQEELEEGKRRPCCWPGCKHRERTGK"
     gene            11300..11452
                     /locus_tag="VFI55_12880"
     CDS             11300..11452
                     /locus_tag="VFI55_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074964.1"
                     /translation="MAAGGAVPALQVRVITDHPEGIDRRRLDAVVELAKPAHAPHRVE
                     VVGRSG"
     gene            complement(11502..>11725)
                     /locus_tag="VFI55_12885"
     CDS             complement(11502..>11725)
                     /locus_tag="VFI55_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074965.1"
                     /translation="CRMALVESPVPPTARHASEELDKDPLILGFALLTHNVQLHGITA
                     DSCVLTHMRLPVIAPSGTGADRHFTTSAS"
BASE COUNT         1813 a         3873 c         3981 g         2058 t
ORIGIN      
        1 cgcgagcagg tggtgacgat gaactcggtc gccaccgcgc tcggcgccgt cgcggccttt
       61 ctgggagccg acttcatgct cgtgccccgc ttcttcttcg gcgccagcga ccggggcgcc
      121 tcggcaatca tcttcatcac cgccattccg gtatcgatcg cgttgctgct gtctttccga
      181 ttcggctccc gcgtcctggg ccccgacgac accaaacgcg caatccacgg atcggtcgtc
      241 tacgcggtga tcaccggctg gctgcacggc gcgcgcacgg tactgcagag tccgacggtc
      301 gccgccacct tgtcgggctt ggcggcacat cgtgcggtga tgggaatcaa ttcgctggtg
      361 atgttggtgc tgctgcacca catgcccgcc ccgcccggcg gcggcttggg gatcgcattg
      421 ctgttcttcg ccgcatcggg cctcggagcg tttctggcca acgtgctgac gccgtcggtg
      481 gtgcggcgct tcggacgcta tgcgacggcg aatggggcct tggcagcagc agcgctcatc
      541 cagatccccg gcgtcgcact gcggctcccg gtgatggcgg tgtgcggctt cctactgggc
      601 gtggccggtc agatggtcaa gctgtgcgcc gactcggcgg tacagatcga cgtcgacgac
      661 gcactgcgcg gacacgtgtt cgcgatccag gatgcgttgt tctgggtctc gttcatcgtg
      721 tcgatcaccg tggctgcgat gttgattccc gacgacgggc acgcgccggc actggtgctg
      781 ttcgggtcgg tgctgtacct aatcgggctc gccgtgcaca gcatcgtagg ccgccggggc
      841 caaccggtga ataatcgctg aggcgtccgc gcgatccccg gacggctgac gatcggaggc
      901 aatgatggct ggtcccgggc cgatagtggc cgacctgcgt gcggaatgcg acgacctcga
      961 cgccttggtg gcgccgctcc ccccagagcg atgggccgat ccgacgccgg cgctgggctg
     1021 gaccatcgcc caccagatcg cacacctgct ctggaccgac gaagtgtccc tgaccgcggt
     1081 caccgacgag gcggggttcg ctgaagtgca gaaggccgct caagccaacc cggagggttt
     1141 cgtcgacgcg gctgccgagg agctggcgat actgccaccg gccgacctgc tgaccgactg
     1201 gcgggtcacc cgcgaacggt tgcacgaggc gctggtgcgg gtgcccgacg gtcgcaagct
     1261 gccgtggttc gggccgccga tgagcgcgac gtcgatggcc accgcgcggt tgatggagac
     1321 ctgggcgcat gggctcgacg tcgctgacgc cgttggcgtc acgcgtcccg ccaccgagcg
     1381 actgcggtcg atcgcgcatc tcggggtacg cacgcgcgat tacgccttcg tcatcaacaa
     1441 tctggccccg ccgaccgaac cattcctggt cgagctgcgt ggacccggcg gcgacgcgtg
     1501 gtcctgggga ccgacggacg ccgcccagcg ggtcacgggc tcggccgagg acttctgcgc
     1561 gctggttacc caacggcgtc cactcagcgc tctcgacatc acggcagaag gtcccgatgc
     1621 gcagcgttgg ctgcagattg ctcaagcctt cgcgggcccg cccggccccg gtcgatgagc
     1681 gccgaaggcg cgcacccgtt ctttcggcgg ctaggggctg gccgccgacg catcgagttt
     1741 tcggaaccag gccaggtgat agtgcgccga tatcgggtct gcggtcgcgg caccttcggt
     1801 ggcggccaac aacagacagt tgagcaacag cgcgggatcg acgttcgggt actggatcac
     1861 cagctgataa cgcgcggtat cgagatgtac ccgcagtaag tcgacctctt cttcgacgac
     1921 gcccgtaccc gcggcggccg ctctggcgag cgtgtgcacc atccgcggca atccgtcgcg
     1981 ccagtgtgtg gcctggctca actcccatcc caggtcgtca accgcgggga gttcccgcgg
     2041 ctgcgctgac gcccgagtag gggcgaaatc atcaagtcgg ccaagggaat ccccgggggc
     2101 gtaagtgagg gtgcgcctgg tctcgccgac cagcgccgag accttgccgc tgcgtcgttc
     2161 gggttccagc agccgcacac ccgccggaag cgtaatgccc ggcggtatcc aaccgtgggc
     2221 gaggtcggtc accaatatcg tggtgccgtc ggcgcgatcg ccgacgcccc aattcagccg
     2281 tggttcttgc tggaccacga acgcgagcag ccgctgcaac cgttcgtcgt cggattcgct
     2341 tgcagcggtt ggcgcaatga cctccggcgt tgcgggggtg atcgccttat cagcgccgtc
     2401 atcgtctgcg gcgcggtgcc ggccctcggg cgcttcgggg ctgtcccgtt caccggcctc
     2461 cgggccagcg accacgccga ccaccggcgc gtcgtcacct tcgctttcga acacttccgg
     2521 ggactcggga gcaccatcca tggacgcgtc ggcagcgggt tcttcgtcgc tgacggcggg
     2581 attgacttgg gtgctttcga gatctgactc agcgccctgc ggacgtgggt gttcggcctg
     2641 gggttcggcc ttcgccgcgt gcagctgacg aagggtcgac tcaactcgtc gcaccgcctg
     2701 tgcacgggcc tcctcgatga ccccgacctc tgccgccagt cgaacggaat cgggcattcc
     2761 cgcacgcttg atgatcttga gctcgtcgtt ggcgctagtc gtgatccgct gcaggtcggc
     2821 cttcagatac tctgcgagcg tggcggtttc ggccgtgcag gtggacagtt cggccggcca
     2881 aaggccgccg gtcacccagg gggtgcaatc gacgggagcg ttgaaagtcg gaattgccag
     2941 tgattcccgg gtagctctcc ggaggcgctg gcgcgccgac atccgaccga agatcgccac
     3001 tggctaagcc taccgagatc tgccgagtgg tccattcagt cggtgaattg tgccgctgcg
     3061 acgacctggg tagcgtgggc acatggctga ttctgcgctg cagcagcaac tcgacgaagt
     3121 gcgggccctg ctcgcccgcg cgcgagagtt gttcggcccc aacccgatag agccacccac
     3181 cgatatcgcg cccaatcccg agagcgcgaa gacctggctg ctgtaggccg tcagctctcg
     3241 ccggaatccg ctcagcgcgc gctatggcgg cggcgtagtt tgtgcgccaa cagcttttca
     3301 atcgccgcat cgaggtcgtg cggtgtcgga acctccatgg acggggtgtg tccggaggcc
     3361 acgatttcgt cgcggacctc cagtaacgcc ttgagacacg acacgtcggc cgttagcaga
     3421 atgccctgcg ccagagtctc ggcgtcggtt tccatcgcct cttcgctcag cgccacgcta
     3481 tgcatgaatc caccgcgaca ggagcggacc aggatgtgcc cgctcggatg gacggtgtcg
     3541 aaggcgggat tggcgtctgt catcgacctc cgtcaacgat gccgccgaaa tttcgagccg
     3601 cgtcgtcctc gtgctgctcc cacatggccg ccgagacgtt gagcttttca gcgatgtcgg
     3661 cgtgatcgtc ggcctgctgc tcgtagcact ggcgtctgag ctcaagcagt tcgcgtccgg
     3721 cggcccgaag ttcactgaag atgggcccca gcgagtccag gctctcctgg atcgccgcat
     3781 gcgacgacgg aatggtgcgc aagtaatcgg aggtctcctg atggtgcgca gcggcttcgc
     3841 gcaagcgcgc gggcaccgcg cggatcgaat ctgccatctc gtgtctcctg ttcatgcgcc
     3901 gccggacgga gccggcgaat tcactattgt cacgtggtcg acgtggccgc cggccgggtg
     3961 ggtgtgggtg tgccgggctt agcgggcgcg ctcgcgggtg cggtcgccgc gggcgggtgc
     4021 tcccagccta ggaagttggg cccgctcacg gtggcgatgt gttgaatctg gccgtcgagc
     4081 acggcttttg cgttgcccac ggcgagcgcg tggcgatcgg tgaagacgcc gatgtcccca
     4141 ggcaggacct gccgcgggtc gatcggattg gtgaccgccg ttcccggtgg gggaatggtg
     4201 attccctgct gttggaaggc atctgcgatc ggtgctccgc cgacagcggc cttgatggcc
     4261 gcggccaact gtggggttgc cgccgtcacc gtctcgccgt cgggcagggt cagcgtcgtc
     4321 gggcccgccg gcgacgattc gggcgtgtcc gccgaggcgt cctcaccgtg ccggtcggtg
     4381 cccgtctcat ccgatgcggg gggatcgctc ggctccgggt cgtgggagcc cggtggcctc
     4441 tggtcgtctg ccgccagcag tgccggatcg gtcctgttgc cgtcgccgaa cccggtaagc
     4501 gggaatccgc ctggctcacc ccaacccgcc atcgagcccg gtgccgcgcc gagacccggc
     4561 agcggtccca cgccgaggtt gggcatggcc ggcatgaccg gcgccggcgg cgttgcagga
     4621 gccgaggcgg cctccgtcga accgtgcccc gacgaatccc cggacaccca gccgggatcg
     4681 tcggccgtca aggaatccag taacgggtcc cagccggtgt cgagatcatc gactggctgt
     4741 tgggcggtag tgccggcgac ggggatggtc gattcgctct tggacgcgtc gtacagcgac
     4801 gtccacgcgg ccatcagcgc cgatttcgac gtgtcatcca ggttcgcgtc caggaccacc
     4861 gtgcgaatgt ctttgagctt gccgatgagg aaccgttgga aatcgcgcgc tcccgccgga
     4921 gtgtcgaggt ctgagcgcgt tcgcaccgcg tcctcggtct ctcgctgcag cttggtcagt
     4981 gcttctctgc cgtcaacggc cttcaagtgt gcgttcagga tggccgaaat cacttgcatg
     5041 tcgagctggg aactcgccga gttctggtgt gccagagcgg cttcggcatc cgccatggcg
     5101 tcggcggcgg cgccctccgc gcggtccgaa gaggcacctg gcgcgtccgg cggccgccac
     5161 gcgggcgggc cccccggtgt cccggcgccg aacaggtcgg aatgttgttg gcggatcttg
     5221 tgcagaattg cgtcgagttg ttcgggcggt gtcatgggcg tgatcacagc ggccacctcg
     5281 ttcggcgcca aacccgcctg ccacgcgttc ggatcgccgg tgctgtcgcg gacgagtttg
     5341 accgtggcaa gtagctcttc gtacgtcgac atcgtctagg caaacggcgc tgcgccccgc
     5401 agcgggatgc ttcgcgaccc ctccatgccg ggcgacagta cccaggcgtg ttcgcgccga
     5461 caattcacct cgatgcggct gtgaacgaac aacccgagcg aggtccgcta gccggcagaa
     5521 accgcgtact gggcccggag cccatccagc acaacccttt tcgcgcgatc cagttcgtgc
     5581 gcatccgcga cgaccgccgc gatctcgcgt tgtttggcag ccaggaactt ctgaaattcg
     5641 cgcgctccca tcggagtatc gagggcagga tcggcccgat cgatggcggc gcgatggatc
     5701 tggtcggcga tcgcgtcgag tcgcctgatg ctttcgcggc tggcggcatg aacgctggcg
     5761 agcacctctg ccagcgcccg gtcggcgtcg gcgaccgtac tgtgttggcg tgccaacgct
     5821 gactgccgcg cctggatggc gtctaccgaa agtccggctt gctctgacat gggttcaacc
     5881 tcttcgtcgt gcccgcgtga gtgggcggtc attgcgtgtc acagcggccg gcgcaccgca
     5941 acgttgttgc ccagcggact gattcgcacc gaagcacccg aatacggagc ctcgatgacc
     6001 aaattggtgc cgatggccag ctgtacgtgt ccggcgtgag ggaaaacgag atcgcccggc
     6061 cggacctgcg agcgggacac cggtatcccg tcgtttatct gctgataggt ggtgcggtcg
     6121 aggtggacac ccgcttgcgc gtacgaccat tggaccaacc cggaacagtc gaactcgtcg
     6181 ggacccgtcg cgccccagac gtacgggcgt cccactcgcg acaacgcggc acgcaccgcg
     6241 attgcggcgc ggccgttgga cgacggcagc cgcaaaccga gaccgcgatg gtgcatcatg
     6301 cggtagcgca gcgcccggag cgccgcggag tgctgttgtg cccgcaagcg ggccgacaag
     6361 acgtgggccc gttgcgtgcg cagtcgggtc acttggcggc gcaccgcctc gcgctgggcc
     6421 atcggcgtca tcggggtagc ggtcgcatcg gcacgggcct cgtcgaggac gcccttggtg
     6481 agctcacccg cctcgactcg atcctggtgt gcgccggcga tgaccgcggc ggcctcggcg
     6541 tccgtgcggg ccgccgacaa aagtcgttgc cggctgtcgt ccgccgcgag ttggtaaccg
     6601 ccacgcccca gaccatgatt gaggccggcg gttcgctgca gcaagccgcg gtaaggcgcg
     6661 gtgcccgcct ccagcgtcgg gtgccaactg ctgccggcaa acaatttgtg cgcgcggcgc
     6721 aacacctcga cgtcgctgtc gatcactgtt ctccctcgcc gctgtcctga tctgcttcgc
     6781 cccggcttgt cgcctcgacc gcctcggtga aggcgcgaac gcgggggagc gccgcagggg
     6841 ggatgactcc ccggttgcgg atgtgccaca tgtcgtcgtt ggcgacgccg accagcgccg
     6901 cgatgatcgg ctccggcaac gacgactgca cgcacgcccg gtcgaaacgt gcactcaagc
     6961 tggtgggcat ccgtttgagc aagtcctggc gcgtcgcctc cagcgcccgc agatgctcca
     7021 tcagccggcc tgtcgagtcg gcgccggcgt tcacggccac ccgccaggag ctggcggcga
     7081 gcatctccgc cttcacacac tgagcgatca cagagagaat atacgtctcg tattcgtttg
     7141 atgtcgtcgc aggcaggcga tcgatgacgg atttgagggc gtccagttgc gcttcggcgt
     7201 agccctccag cacatcggtg tcgctgtggc gatccacgac caccgcgccg ccaggggccg
     7261 caggcctgtc gtcaggtggt cgcgcggcca tcactcgggc cagttctgca tcggggtctg
     7321 cggccaccaa cagcctggag taggtgcccg gcggcaggcc cagtccattt tcgatcttct
     7381 gaactgtgct tttatgcggc tttcgggcac cgcgctccag aaagctcagc cccataatgc
     7441 tgaccccggt cgcggcggcg aggtcggcta gcgaccagtc gcgggactcg cgcagcgcgc
     7501 ggatcgctgc accggccgac tcacggctca ccgcacgctc cggccatagc cggatagtac
     7561 acagaccgag accgattgct atgtatatac gtttctgtca cgtttctctt gcgccaggcg
     7621 accgcgtgca tatacgcttc tgaatacgtt tcacctcaca aggagaccga tatgggaaat
     7681 ccctgcgcag ccaacccgga actttggttc ggctatcccg acgacgacgg tggtgacggt
     7741 gcggcaaagg cacgcgctta tgagcggtcg gccaccgagg cacggatcca atgcctgcgc
     7801 cgctgcccgt tggcggaaca acgccgctgc gcccagcacg ccatcgcgca ccgcgaggag
     7861 tacggcgtct gggccggcgt caagctaccc ggcggccagt accgcaagcg cgaacagctc
     7921 gcgcgggccc acgacatact gcggcgcatc gccgccggcg agatcaattc gaggcagctg
     7981 cccgaaaacg cggcgctgct gacccgtcac gagcacgacg ccgttgcggt gaccgcggtg
     8041 gtgctgcacc tgccgaccgc acaagttggg ccgcgttcgg ccgcctgacc atcggttggc
     8101 gtgctctgcc gaggagtttg ctgagccggc caacggggta gacgggcttg ggtacgaccg
     8161 agaccgtgga ctctggggcg cagccatgac gttcgacctg acaccgacgg cggcacaaca
     8221 cgacctggcc cggcgtaccc acgagttcgc cgaggaagtg gtccgcccgg tggcgctcga
     8281 gtacgaccag cggcaggagt ttccgtggcc cgtgctggag gaggcggccc gccgggggtt
     8341 ctacagcccg ttgttttacc gcgacttgat cggtgatccg accggcatct cgctaccgat
     8401 gttcatggag gagctgttct ggggctgcgc cggaatcggt ttggcgatcg tcatgccggc
     8461 gctggcgctg tcggcgatcg gtcaggccgc gtcgccggag cagatgcttg cgtgggcacc
     8521 cgaatgtttc ggaacaccag gcgatctcaa acttgccgcc ctggcgatct ccgagccaga
     8581 gggtggcagc gacgtgcgga atctacgcac ccgggcgcgc cgcgatggtg acgactggat
     8641 catcgacggc cacaagatgt ggatcggcaa cggcggtatc gccaacgtgc acgtcgtcaa
     8701 cgcggtggtc gacgaggaac tcggccaccg cggccaggcg ctgttcgtcg tgcccggagg
     8761 catccccggg ctgaaaatgg tgcgcaagct cgacaagctg ggctgccgcg cgtcccacac
     8821 cgccgagttg ctgtttgacg gcgtccgcgt tcccgcggcc aatctgcttg ggggagaaga
     8881 gaagctcgag cacaaactcg cgaaggcccg cgaggcggtc gcgggcggac agcgttccgg
     8941 ctcggcgaca ttgggcacct tcgagcagac gcgcccgatg gtggccgccc aagccatcgg
     9001 gattgctcgc gccgcgctgg agtacgcgac gcagtacgcc accgagcggg aggcgttcgg
     9061 cgggccgatc atcgacaacc agggcatcgc gtttccgctc gcggacctgg ccaccgagat
     9121 cgacgccgct cgactgctga cctggcgtgc gtcgtggatg gcggctagcg gggtgccctt
     9181 cgagcggggt gagggctcga tgtcgaagct cgccgccagc gaagtcgccg tcaaggccac
     9241 cgaacgcgct attcagacca tgggcggctg gggctacatc accgaccatc cggtcgagaa
     9301 gtggtatcgg gatgccaagc tgtacaccat ctttgagggc accagcgaga ttcagcggat
     9361 ggtcgtctcc aacgcgctgg gcgccgccgt gggcaccccg ccgctgcatg tggtgatcga
     9421 gccgaccggc gggccgctga accggatttt cggtcgcggc acgccattgc ggtcccgcgc
     9481 cgccgatgtg gcgctgtcga tgcaggaccg cgtgcccccg cccgtcatgc ggctggccat
     9541 gaagctgcta cgcccgccag gcaggtgagc gggccagggg tactgtcgcc gtatcggccc
     9601 gcatcaggct catgacggag ggtggacgat gagcaacctc gaagccgccc ccgccgaagt
     9661 cgcctgcact gcttcacggg tcaccgggat cgaagtgctg cagccgcgag aagtgccgct
     9721 gggtggaccc cgcgcgatgc acgtgcaacg caccctgccg caacaacaac gctcgctgat
     9781 cggcgcctgg tgctttatcg accactacgg cccggcgtcg gcccgcatgg acgtcccgcc
     9841 tcatccgcac accggattac aaaccgtgag ttggttgttc agtggggaag tggagcatcg
     9901 cgacagcgca ggcgtccatg ccatggtccg acccagcgag ctgaatctga tgacggcggg
     9961 cgcgggcatt tgtcactccg aggtgtcgat cgagtcgaac accgctttgc acggtgtgca
    10021 gctgtgggtg gcgctgcctg attccgatcg cgacaccgga cgcgatttcg ctcactatga
    10081 gccgccgccg gtttccctgg gtggtgccgt ggcgcgcgtg tttttggggg agctggccgg
    10141 ccaccgatcg cccgtgcaca cgttcactcc gctactgggc gcccagatcg atctcgatgt
    10201 gaacgccggg ctggacattg acctggatcc ggcgttcgag cacggcgtgc tctgcgatgc
    10261 ggggcccgtc gagatgggcg gggttgccct ggcagccgcc gaactcgggt accaaagccc
    10321 gggcagctcg tcgctgcacc tgcgcaatgt tggcgatcgg ccggcgcgcg tggtgctgct
    10381 gggcgggacc ccgttcaccg aacagttggt gatgtggtgg aacttcgtcg ggcgcagtca
    10441 cgacgacatc gtcggctacc gccagctgtg ggaggacgcc gacgaccgct ttggcgccgt
    10501 ccacggatac cggggctcgg ttgcccgact acccgccccg ccgctaccca tcacccggct
    10561 gctgccacga ccaataccgc aaagaaaggg acacacatga ccgtcgataa agccggcgcg
    10621 caaaccacag tcaccgaaga agaggatcgg tacaccatcg ccgtcgacgg caagctggtc
    10681 ggcctcgccg atttcgctga ccgcggcgat cagcgcgtct tctatcacac cgagatcgac
    10741 cccgcgtacg gcggacgtgg cctggggacc atcctcgtcg agggggcact gaacgcgacg
    10801 cgggatgagg gcaagcgcgt cgttccggtg tgctccatgt ttgtcacgac actcaagaag
    10861 catcccgagt tcgacgacat caccgatccc gtcactggcg acattcagca gtggttgcac
    10921 agccagcccg ccggttaggc gcgggctgga aggcttgagc gtgacggccg ccccggtggt
    10981 gtcggggcgg gcgcctattt gccggtccgc tcgcggtgct tgcagcccgg ccagcagcag
    11041 ggccgtcgct tgccttcttc cagctcttcc tgggtccggc gaatgcggcg cgttcgggtc
    11101 acgtcttgct tggcgtcttc gacccagcag atgaactcgt tgcgcgccaa tggcgggatg
    11161 tccttccacg cggcgagtgc tacggagttg gcgatcaacg cttcgcgcat gtcggtgggt
    11221 aacttgtgca ccgcaccacc gggcactcgc tcgctgctca cggagttagg gtaaggcgac
    11281 cgagccccaa gaggcaccag tggctgccgg tggcgcggtg cccgctctgc aggttcgggt
    11341 catcaccgat catcccgaag gcatcgaccg ccggcgtctc gacgcggtcg tggagttggc
    11401 caagccggcc catgcgcccc atcgcgtcga ggtggtcggt cggagcggtt gatcagcgga
    11461 cattttgcac gggcccggcc acgccggttc ccgtccgcat atcagctggc tgaggtcgtg
    11521 aaatgccgat ctgcgcccgt ccccgacggc gcgataaccg gcaaacgcat gtgtgtgaga
    11581 acacaagaat cagcggtaat gccatgtaac tgaacattgt gcgtcagcag cgcgaacccg
    11641 agaattaaag ggtccttgtc caactcttcg gacgcgtgcc gtgcggttgg tggcaccggc
    11701 gattccacca gggccatccg gcacc
//
LOCUS       DASSGQ010000291        17225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_443695,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000291 DASSGQ010000000
VERSION     DASSGQ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 17225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17225
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443695"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1049
                     /locus_tag="VFI55_12890"
     CDS             3..1049
                     /locus_tag="VFI55_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HET7074966.1"
                     /translation="MLVTVAALALGYAHGGLNALFLLLVLAVLEVSLSFDNAIINASI
                     LKRMSQFWRQMFLTIGILIAVFGMRLIFPLGIVWASAGIDPVRAMTLALHPPPHGALE
                     YPDGSPSYEKLVLAAHPQIAAFGGIFLLMLFLDFVFHDRDIKWLKWIEVPFARVGRLG
                     QVPVVVAGILLVLVGALLTHSSDERAAVLTAGLLGLISYLVVNGFSRAFRPPDIEAGM
                     SGKVVGTGGLMLFLYLEVLDAAFSFDGVTGAFAITSDPILIALGLGLIGSMFVRSITI
                     YLVQQETLDRYVYLEHGAHWAIGALAVIMLLSIEPRFEVPDAVTASVGVVFIGAALGW
                     SVLQSRRTARGFPG"
     gene            complement(1065..1757)
                     /locus_tag="VFI55_12895"
     CDS             complement(1065..1757)
                     /locus_tag="VFI55_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7074967.1"
                     /translation="MTPMLHYAAGFWWLIFPLGGAIGTGLKAIAAANERRAERRLERY
                     RLKQQARIAVAEASVRERNSEDSSRRKLAKLIATHDRTDARWFSYEVDIAKLLDFPMM
                     TDMRQPLTIAFHKAKRHADLLRPERPEDLAGDRVARLEYRDAVHEYISAFEIAEAEAI
                     RRRRSDFSKDEQQRLARAENLLRLAQDEAATREERQSAYARAGKELDGLIMMPAPARA
                     SIERRIAGQIEA"
     gene            complement(1835..2323)
                     /locus_tag="VFI55_12900"
     CDS             complement(1835..2323)
                     /locus_tag="VFI55_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET7074968.1"
                     /translation="MQFDVTIEIPKGQRNKYEVDHETGRVRLDRYLYTSMAYPTDYGF
                     IEDTLGEDGDPLDALVLLPAPLFPGVLVEARPVGMFQMVDEAGGDDKVLCVPAGDHRW
                     DHIQDIGDVPEFELDVIKHFFSQYKALEPGKYVKTANWVDRAAAEAEVQRSVERFKTT
                     EH"
     gene            2438..3823
                     /gene="dacB"
                     /locus_tag="VFI55_12905"
     CDS             2438..3823
                     /gene="dacB"
                     /locus_tag="VFI55_12905"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HET7074969.1"
                     /translation="MGPTRRRKSTQVFIGLAVLAFVAAVVAAATYFTTGMHSAGATHV
                     QVPPPRPPIVKAGVVPVADTAEAPSPGGVAATLAAVAADPNLGRLGGRITDAITGKEL
                     WQVADDMPLVPASTNKVLTAAAALLTLDRQARVSTRVVAGGPNAQGPVVLVGAGDPTL
                     SAAPPGVDTWYKGAPRISDLVEQVRRSGVTPTAVQVDTSAFTGPTLAQGWDPSDVDNG
                     DIAPIESVMIDAGRIQPSTVDSRRSPTPALDAGRELAKGLGLDPAAVTIAAAPSGARQ
                     LAVVQSAPLVQRLSEMMNASDNVMAECIGREVAAAINRPRSFAGAVDAVTNRLSVAHV
                     DTTGAALMDSSGLSVDDRLTAKTLDGVVQAAAGPDQPALRALLDLLPIAAGSGTLVDR
                     FVDTATNQGPAGWLRAKTGSLTAINSLVGVLTDRSGRVLTFAFISNAAGPNGRNAMDA
                     VAARLWSCGCA"
     gene            3820..4893
                     /locus_tag="VFI55_12910"
     CDS             3820..4893
                     /locus_tag="VFI55_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HET7074970.1"
                     /translation="MTTSSNLTVGTAVDWRFAATVGQRLARPGPASSDYTRRQVVDEL
                     TSAAAKAEPPVRDVTGLVTKGVVPPARVVDRGEWIGAAAESMRVMTNGGEKPRGFVTG
                     RITGAQTGAVLAFVSSGILGQYDPFASQKEGRKQGEKEGCLLLVYPNVLAVERQLRVE
                     PSDFRLWVCLHEVTHRVQFTANPWLSDYMSQALGLLTQDAGEDIGQVARRLADFVRNR
                     GDESLSDGSSPGILGLVRAVQSEPQRKALDQLLVLGTLLEGHADHVMDAVGPVVVPSV
                     ATIRRRFDERRHRKQPPLQRLLRALLGLDAKLSQYTRGKAFVDHVVGRVGMARFNTIW
                     SGPETLPLPAEIEDPQRWIDRVL"
     gene            4872..5831
                     /gene="tilS"
                     /locus_tag="VFI55_12915"
     CDS             4872..5831
                     /gene="tilS"
                     /locus_tag="VFI55_12915"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET7074971.1"
                     /translation="MDRQGAVGLLRGAVEAFAKTYLATEKQWCVALSGGPDSLALTAV
                     AAPLRPTTALIVDHGLQPDSATVAETARKQAVDLGCVDAQVICVDVGRDGGPEAAART
                     ARYAALGTHRRGPVLLAHTLDDQAETVLLGLGRGSGMRSIAGMRPHDPPWCRPLLGVR
                     RSVTHAACRELGLTAWQDPHNTDRRFTRTRLRLDVLPLLEDVLGGGVAEALARTATAL
                     REDSQLIDSLAAHALPEVTAGRGLQALALAALPGPVRRRVIRGWLLAGGATGLTDKQI
                     RGVDTLVTAWRGQGGVAVGSSLRGERLIAGRRDGVLTLWREPV"
     gene            5875..6486
                     /gene="hpt"
                     /locus_tag="VFI55_12920"
     CDS             5875..6486
                     /gene="hpt"
                     /locus_tag="VFI55_12920"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET7074972.1"
                     /translation="MVVTQTSSAITPAHPAELYPGDIKSVLLTTEQIQARVAELGAQI
                     GNDYRDVAADTGQDLLLITVLKGAVIFVTDLARAIPVPTQFEFMAVSSYGSSTSSSGV
                     VRILKDLDRDINDRDVLIVEDVVDSGLTLSWLSRNLTTRHPRSLRVCTLLRKPDAMNA
                     NVNIAYVGFDIPNEFVVGYGLDYDERYRDLSYIGTLDPRIYEA"
     gene            complement(6548..7297)
                     /locus_tag="VFI55_12925"
     CDS             complement(6548..7297)
                     /locus_tag="VFI55_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723624.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HET7074973.1"
                     /translation="MPIGTNAPRLVRTSIAVAAAGLAAVATLSGCVKHNPASPAANPR
                     QVTVVGSGQVQGVPDTLTADAGIEFTAPDVSTAMNQTNDRQQAVINALVGAGLDRKDI
                     ATTTVTLQPQYGNPEPNGTATITGYRATNAIQVKIHPTDTASRMLALIVSTGGDATRI
                     SSVSYSIADDSQLVKDARARAFNDAKGRADQYAQLSGLRLGRVLSISEVAGGGPVAGA
                     PPVPQRGIAAVPLEPGQQTVSFSVTAVWELD"
     gene            7366..8509
                     /locus_tag="VFI55_12930"
                     /pseudo
     CDS             7366..8509
                     /locus_tag="VFI55_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815921.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
     gene            complement(8886..9902)
                     /locus_tag="VFI55_12935"
     CDS             complement(8886..9902)
                     /locus_tag="VFI55_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7074974.1"
                     /translation="MRAAVLRDGRMVYRDDVPEPVPEPGQVLVAVRACGICGSDLHFA
                     AHGAEVLALGDRLGGGAASGMNLDMTSDVFMGHEFSAEVLEAGPDTETHPPGTPVTSI
                     PVLLSAKGVQPIGYSNSTLSGYAERMLLSAPLLLPIPNGLDLKHAALTEPMAVGLHAV
                     NKSDIAPNETALVLGCGPIGIAIIAALRARGIETIVASDFSPIRRELAATMGAHRTLD
                     AAQGSPFDTVKAAVVFEAVGVPGIIDDVLLRARPGTRLVVAGVCMQPDTVHPFFAIAK
                     EINVQFVFAYSPDEFADSLRALAEGEIDVAPLITGEAGLEGVGQAFDDLADPERHCKI
                     LVTT"
     gene            complement(9902..11089)
                     /locus_tag="VFI55_12940"
     CDS             complement(9902..11089)
                     /locus_tag="VFI55_12940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7074975.1"
                     /translation="MSAPLRFGVFITPFHPVGQSPTVALEYDMERVVALDRLGFDEAW
                     FGEHHSGGYELIACPEVFIAAAAERTKHIRLGTGVVSLPYHHPLMVADRWVLLDHLTR
                     GRVMFGTGPGALPSDAYMMGIDPVEQRRMMQESLEAILALFRAGPTERIDRHSDWFTL
                     RDAQLHIRPYTWPYPEIAAAAMISPSGPRLAGALGTSLLSLSMSVPGGYAALENTWEV
                     VRAQAAKVGRDEPDRVNWRVLSIMHIGDSREQAIDDCTYGLQDFANYFGAAGFVPLAN
                     TVEGAQSPQEFVEDYAAKGNCCIGTPEDAIAHIEDLLERSGGFGTLLMLGHDWASPEA
                     TYHCYDLMARKVIPHFKGQLEAARSSHDWARGKRDQLIGRAGEAVVKAITEHVDEQKS
                     VGN"
     gene            complement(11086..12054)
                     /locus_tag="VFI55_12945"
     CDS             complement(11086..12054)
                     /locus_tag="VFI55_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7074976.1"
                     /translation="MPISTERLVDTNGVQLRVTEAGDRGAPVVVLAHGFPELAYSWRH
                     QIPALAEAGYHVLAPDQRGYGGSSRPDAIEAYNIRELTADVVGLLDDVGAERAVWIGH
                     DWGAAVVWNAPLLHPDRVAAVAALSVPVVPRAKVPPTQAFRATFGENFFYILYFQEPG
                     VADAELDADPARTMRRMMGGLTVSGDHSAGMRMVAPGPEGFLDRLPEPERLPDWLSQD
                     ELDHYISEFARTGFTGGLNWYRNFDRNWETTAELTGAKIAVPSLFIAGTADPVLAFTR
                     TDRALEVISGPYRQVMIDGAGHWLQQERPDEVNATLLEFLKGLELR"
     gene            12148..12564
                     /locus_tag="VFI55_12950"
     CDS             12148..12564
                     /locus_tag="VFI55_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7074977.1"
                     /translation="MRRLDHIVLWTHNLQASMDFYTRVVGLSPVRFDEFAAGEAPFPS
                     VRVCEDSIIDLSPVDNSAGTESLTGVGGSAGHQVNHVCIAFSKSEYDALDQRLRAEGV
                     DTSARLSPSFGARGWAPQGYYFSDPDGNVVEARYYD"
     gene            12775..15165
                     /gene="ftsH"
                     /locus_tag="VFI55_12955"
     CDS             12775..15165
                     /gene="ftsH"
                     /locus_tag="VFI55_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET7074978.1"
                     /translation="MNRKNVIRTLTAIAVIVLLGWSFFYFSDDTRGYKPVDTSVAMTQ
                     INSDNVASAQIDDREQQLRLTLKKANGDTDNSDKVITKYPTGYAVDLLNALTAKNAKV
                     STTVNEGSILGELLVYVLPLLLLVGLFVMFSRMQGGARMGFGFGKSRAKQLSKDMPKT
                     TFADVAGVDEAVEELYEIKDFLQNPARYQALGAKIPKGVLLYGPPGTGKTLLARAVAG
                     EAGVPFFTISGSDFVEMFVGVGASRVRDLFEQAKQNSPCIIFVDEIDAVGRQRGAGLG
                     GGHDEREQTLNQLLVEMDGFGERAGVILIAATNRPDILDPALLRPGRFDRQIPVSNPD
                     LAGRRAVLRVHSKGKPIAPDADLDGLAKRTVGMTGADLANVINEAALLTARENGTVIT
                     GPALEEAVDRVIGGPRRKGRIISEQEKKITAYHEGGHTLAAWAMPDIDPVYKVTILAR
                     GRTGGHAVAVPEEDKGLRTRSEMIAQLVFAMGGRAAEELVFREPTTGAVSDIEQATKI
                     ARSMVTEFGMSSKLGAVKYGTEHGDPFLGRTMGTQADYSHEVARDIDDEVRKLIEAAH
                     TEAWEILTEYRDVLDALAGELLEKETLHRPELQAIFGDVEKRPRLTMFDDFGGRIPSD
                     KPPIKTPGELAIERGEPWPPPVVEPAFKAAIAQASQAVEAGQTDADRNAHGGNGFHDG
                     HGAGNRQATPPGATQPDYGAPAGWYAPGWPPQQQQPNSWHPPAQSPQQPYWPQPAGSY
                     PGYPGPHVQPGQHGQAHPSYPAYPPYAAPGPSDADAGKSPDQPDDGVSRSNPPAHG"
     gene            15181..15813
                     /gene="folE"
                     /locus_tag="VFI55_12960"
     CDS             15181..15813
                     /gene="folE"
                     /locus_tag="VFI55_12960"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET7074979.1"
                     /translation="MALPDLGPDLGPDSRTAPFRIRTFDQQRAEAAVRELLLAIGEDP
                     ERDGLQDTPARVARAYREMFAGLYTDPDTVLNTMFDEEHDELVIVKEIPLYSTCEHHL
                     VSFHGVAHVGYIPGDEGRVTGLSKIARLVDLYAKRPQVQERLTSQIADALVKKLDPRG
                     AIVVIEAEHLCMAMRGVRKPGAVTTTSAVRGLFKTNAASRAEALDLILHK"
     gene            15810..16637
                     /gene="folP"
                     /locus_tag="VFI55_12965"
     CDS             15810..16637
                     /gene="folP"
                     /locus_tag="VFI55_12965"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET7074980.1"
                     /translation="MSPAPVQVMGVLNITHDSFSDGGRYLDADDAVAHGLAMAAEGAE
                     IVDVGGESTRPGATRVDPRVEASRVLPVVKELAAQGITVSIDTMHAEVARAALQSGAR
                     IVNDVSGGRADPAMAPLLAEAGVPWVLMHWRPVSFDRPHEAPHYRDVVAEVRAELLAA
                     VDDAVAAGVDPAQLVIDPGLGFAKTGQHNWALLHALPQLVATGIPVLLGASRKRFLGT
                     LLAAPDGSPRPPDGRETATATISALAALHGAWGVRVHDVRASVDALKVVGAWTGCDG"
     gene            16630..17031
                     /gene="folB"
                     /locus_tag="VFI55_12970"
     CDS             16630..17031
                     /gene="folB"
                     /locus_tag="VFI55_12970"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HET7074981.1"
                     /translation="MADRIELRGLTIRGRHGVFDHERANGQDFVVDITVWIDLADAGA
                     SDDLADTYDYAALAQLAADVVAGPPRNLIEAVGGDIADQVMADHRVHAVEVVLHKPRA
                     PIPHQFADVAVVVRRSRRGGRGSVIPAGGAL"
     gene            17096..>17225
                     /locus_tag="VFI55_12975"
     CDS             17096..>17225
                     /locus_tag="VFI55_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET7074982.1"
                     /translation="MSRVVLSVGSNLGDRLARLQSVVDGLEGELVAVSPVYETDPWG"
BASE COUNT         2712 a         5751 c         5885 g         2877 t
ORIGIN      
        1 cgctgttggt caccgtggcg gcccttgccc tcggatacgc gcacggcggc ctgaacgccc
       61 tctttttgct gctggtgctg gccgtcctcg aggtgtcact gtcgttcgac aacgcgatta
      121 tcaacgcctc gatcctcaag cggatgagcc agttctggcg gcagatgttc ctgacgatcg
      181 ggattctcat cgcggtgttc gggatgcggc tgatcttccc cttgggcatc gtgtgggcca
      241 gcgcgggaat cgacccggtt cgcgcgatga cgctggcgct gcatccgccg ccgcacggcg
      301 cgctggaata cccggacggc tcacccagct acgagaagct ggtcctggcc gcccacccgc
      361 agatcgcggc gttcggcggg atcttcctgt tgatgttgtt tttggacttc gtctttcatg
      421 accgagatat caagtggctc aagtggatcg aggtaccctt cgcccgcgtc gggcggcttg
      481 gccaggtgcc ggtggtggtg gccggcattc tgcttgtcct cgtgggcgcg ttgctgacgc
      541 actccagcga cgagcgcgcg gcggtactga ccgccggact gctgggcctg atcagctatc
      601 tcgtcgtcaa cggtttcagc cgggcgtttc gcccgccaga catcgaggcc ggcatgtcgg
      661 gcaaggtggt cggcacgggc ggcctgatgc tgtttctcta cctcgaggtt ctcgacgccg
      721 ccttctcgtt cgacggggtc accggcgcgt tcgcgatcac gtcagacccg attctcatcg
      781 cgctgggtct ggggctgatc ggctcgatgt tcgtccggtc gatcaccatc tacctggtcc
      841 agcaggaaac cctggaccgt tacgtgtacc tggaacacgg cgcgcactgg gcgatcggcg
      901 cgctggccgt gatcatgctg ctctccatcg agccacgctt cgaagtcccc gatgcggtca
      961 ccgcctcggt gggggtggtc ttcatcgggg cggcattggg ctggagtgtg ttgcagagca
     1021 ggcgtactgc ccgtggcttc cccggctagg cctcgattga ccggctaggc ctcgatctgg
     1081 ccggcgatgc gccgttcgat gctggccctc gccggggcgg gcatcatgat cagtccgtcg
     1141 agttccttgc cggctcgcgc gtaggcgctc tgtcgctcct cacgcgtcgc cgcctcgtct
     1201 tgagccaggc gcagcaggtt ctcggcgcgt gccaggcgtt gttgttcgtc tttcgagaag
     1261 tcgctgcgcc gccggcgaat cgcctcggct tcggcgatct cgaacgcgct gatgtactcg
     1321 tggactgcgt cgcggtattc cagccgggcc acccggtcgc cggcgaggtc ctcgggccgt
     1381 tcgggccgaa gcaggtccgc gtgccgtttc gccttgtgaa acgcgatggt cagcggctgg
     1441 cgcatgtcgg tcatcatcgg gaagtccagc aatttggcga tgtcgacttc gtagctgaac
     1501 caccgcgcgt cggtacggtc atgcgtcgca atgagtttgg cgagctttcg gcgcgagctg
     1561 tcctcgctgt ttcgctcccg cacggacgcc tccgccaccg cgatcctggc ttgctgcttg
     1621 aggcggtagc gctccagccg tcgttcggcc cgacgctcgt tcgcggcggc gatcgccttg
     1681 aggcccgtac cgatcgcgcc acccagcggg aagatcagcc accagaagcc ggcggcgtaa
     1741 tgcagcatcg gggtcatatc gccaggatgc catcgagatg cggcgcgcgg ttgcgccagc
     1801 agcgtcagta cacgtcagtg cacgttagtg cgcgctagtg ttcagtggtc ttgaaacgct
     1861 ccaccgaacg ctgcacctcc gcttcggccg cggcgcggtc aacccagttg gccgtcttga
     1921 cgtacttgcc cggctccagg gccttgtatt gcgagaaaaa gtgcttgatc acgtccagtt
     1981 cgaattccgg gacgtctccg atgtcctgga tgtgatccca gcggtggtcg ccggccggga
     2041 cgcacaacac cttgtcgtcg ccgccggcct cgtcgaccat ctggaacatc cccaccgggc
     2101 gggcctccac cagcacgccg gggaaaagcg gcgccggcag cagcaccagc gcgtcgagcg
     2161 ggtcgccgtc ttcaccgagg gtgtcctcga tgaagccgta gtcggtcggg tacgccatcg
     2221 aggtgtacag gtagcggtcc agacgtaccc ggcccgtctc gtggtcgacc tcgtacttgt
     2281 tgcgctggcc cttcggaatt tcgatggtca cgtcgaattg caccgcgtcg gctccttcgc
     2341 gaggggtggg tctgggtagt cagttgcgtc tcgcatcacc ctagtcgagc gcattgcggt
     2401 cgattcggca gaatgggttc aaacagtcag gagagtgatg ggtccgactc gccggcgaaa
     2461 atccacccag gttttcatcg gattggccgt gctggcgttt gtcgctgccg tggtggcggc
     2521 ggcaacatac ttcaccacgg gtatgcacag cgccggcgcc acccatgtcc aggtgccgcc
     2581 tccgcgcccg ccgatcgtca aggcaggcgt cgtcccggtc gccgacacgg ccgaggcccc
     2641 cagccccggc ggggttgccg cgacgctcgc ggccgtggcg gccgacccca atctgggcag
     2701 gctgggcggt cggatcaccg atgccatcac cggaaaagag ctgtggcagg tagctgacga
     2761 catgccgctg gtgccggcgt cgaccaacaa agtcctcacc gcggcggcgg cgttgctgac
     2821 cttggaccgt caggcccggg tcagcactcg ggtggtcgcc ggtggcccga acgcccaggg
     2881 gccggtggtg ctggtgggtg ccggggatcc gacgctctcg gcggcaccgc ccggcgtgga
     2941 cacctggtac aagggtgcgc cgcgaatcag cgacctcgtc gaacaggtgc ggcgcagcgg
     3001 cgtgacgccg accgcagtgc aggtcgacac ctcggcgttc actggaccca ctttggcgca
     3061 gggctgggat ccctccgacg tcgacaatgg cgacatcgcg ccgatcgagt cggtgatgat
     3121 cgacgccggc cgcatccagc cgagcaccgt cgactccaga aggtcgccga cgccggcgct
     3181 ggatgccggg cgggaactgg ccaagggcct cggtctggac cctgccgcgg tgacgatcgc
     3241 cgcggcgccg tccggtgcgc ggcagctggc cgtcgtgcag tctgcgccgc tggtccagcg
     3301 gctctcggag atgatgaacg cctccgacaa cgtgatggcc gaatgcatcg ggcgggaggt
     3361 ggcggccgcg atcaatcgac cgcgcagctt cgcgggcgcc gtcgacgcgg tgaccaaccg
     3421 gttgagcgtc gcgcacgtcg acaccaccgg ggctgcgctg atggattcca gcgggctttc
     3481 ggtcgacgac cggttgaccg ccaagacgct cgacggggtg gtgcaggcgg ccgccggacc
     3541 ggaccagccg gcgctgcggg cgctgctgga tctgcttccg atcgccgccg gcagcggcac
     3601 gctggtcgac cgcttcgtcg acaccgccac caaccagggc ccggccggct ggctgcgtgc
     3661 caagacgggc tcgttgaccg ccatcaactc gctggtcggg gtgctcaccg accgcagcgg
     3721 acgggtgctg acgttcgcgt tcatctccaa cgcggccggc ccgaacggcc gcaacgcgat
     3781 ggacgccgtg gccgccaggc tctggtcgtg cggctgcgca tgaccacatc gtcaaacctc
     3841 accgtcggca ccgctgtcga ctggcggttc gcggccaccg tcggccaacg gctggccagg
     3901 cccggcccgg cgtccagcga ctacacccgc cgccaggtgg tcgacgaact gacgagtgcg
     3961 gccgcgaagg cagaaccccc ggtgcgtgac gtcacgggcc tggtcaccaa gggcgtggtt
     4021 ccgcccgccc gggtcgtcga ccgcggggag tggatcggcg cggccgccga gtcgatgcgg
     4081 gtgatgacga acggaggcga aaagccgcgg ggctttgtca ccggccgcat caccggtgcc
     4141 cagacgggcg ccgtgctggc gttcgtgtct tccgggatcc tcggccagta cgatccgttc
     4201 gcctcgcaaa aagaggggcg aaaacaaggc gaaaaagagg gctgcctgct gctggtgtat
     4261 ccgaacgtcc tcgccgtcga gcgtcagctt cgggtcgagc cttccgattt ccggttgtgg
     4321 gtatgcctgc acgaggtgac ccaccgggtc cagttcacgg ccaacccctg gttgtcggat
     4381 tacatgtcgc aggcgttggg actgctgacc caggacgcgg gggaagacat cgggcaggtg
     4441 gccaggcggc tcgccgactt cgtccgcaac cgcggcgacg agtcgctgtc cgacggtagc
     4501 tcgccgggga tcctcggcct ggtgcgcgcc gtgcagtccg agccgcaacg caaagcgctc
     4561 gatcagctgc tggtgctcgg cacgctgctg gagggccacg ccgatcacgt gatggatgcg
     4621 gtcgggccgg tagtcgtgcc gtcggtagcc accatccgtc gtcgattcga cgagcgtcgc
     4681 catcgcaaac aaccgccgct gcagcgcctg ctgcgcgcac tgctggggct cgacgccaag
     4741 ctcagccagt acacccgcgg caaagcgttc gtcgaccacg tggtgggccg ggtcgggatg
     4801 gcgcggttca acaccatctg gtcgggcccc gaaacgctgc cactgcccgc cgagatcgaa
     4861 gaccctcagc ggtggatcga cagggtgctg tagggctgct gcgcggggcg gttgaggcgt
     4921 tcgccaagac ctacctcgcc acggaaaagc aatggtgcgt ggcgctgtcc ggcggaccgg
     4981 actcgctggc gctcaccgcg gtcgcggccc cgctgcgtcc caccacggcg ctgatcgtgg
     5041 atcacggtct gcagcccgac tcggccaccg tcgccgagac cgcgcgcaag caggcagtcg
     5101 atttgggatg cgttgacgca caggtgattt gcgtcgacgt tggccgtgac ggcggtcccg
     5161 aggcggcggc gcgcaccgcc cgctacgccg cgttgggcac gcaccgtcgc ggtcccgtgc
     5221 tgttggcgca cacgcttgac gaccaggcgg agacggtatt gctggggctg ggccgcggct
     5281 ccgggatgcg ctcgatcgcc gggatgcggc cgcacgaccc accgtggtgc cgtccgttgc
     5341 tgggggtgcg gcgcagcgtg acgcacgccg cgtgccgcga actgggcctg accgcgtggc
     5401 aggacccgca caacaccgac cgccgcttca cccgcacccg gctgcgcctg gacgtgctgc
     5461 cgttgctgga ggacgtgctg ggcggcgggg tggccgaggc cctggcccgc accgcgacgg
     5521 cgttgcgcga ggactcacag ctgatcgact cgctcgccgc acatgcgctg cccgaggtga
     5581 cggcggggcg cgggctgcag gccctggctt tggcggcgtt gcccgggccg gtgcgacgcc
     5641 gggtgatccg cggctggcta ctggccgggg gcgccaccgg gctgaccgac aagcagattc
     5701 gcggcgtgga cacgctggtc accgcctggc gcgggcaggg cggagtggcc gtgggctcgt
     5761 cgttgcgcgg cgagcgattg atcgccggac gacgcgacgg tgtgctgaca ttgtggcgcg
     5821 agcctgtgta aatcgcggct gccgttggtt attcgcccgt tcgcgtggca ctctgtggtc
     5881 gtgacccaga cgtcctcggc gatcaccccc gctcaccccg cggagctgta cccgggggat
     5941 atcaagtcgg tgctgctgac cacggagcag attcaggccc gcgtcgccga gctcggcgcg
     6001 caaatcggca acgactatcg ggacgtcgca gccgacaccg gccaagacct gctgctgatc
     6061 accgtgctca agggcgccgt gatcttcgtc accgacctgg ctcgcgcgat tccggtgccg
     6121 acgcagttcg agttcatggc agtcagctcc tacgggtcct cgacctcgtc gtcgggcgtg
     6181 gtgcggatcc tcaaggacct ggaccgcgac atcaacgatc gcgacgtgct gattgtcgag
     6241 gacgtcgtcg actcgggact gaccttgtcg tggctatcgc ggaacctcac cacccggcat
     6301 ccacgctcgc tgcgggtgtg cacgctgttg cgcaagcccg acgcgatgaa cgccaacgtc
     6361 aacatcgcct acgtgggctt cgacattccc aacgagttcg tcgttggcta cggcctggat
     6421 tacgacgagc gctaccgcga cctgtcctac atcgggacct tggaccccag gatctacgag
     6481 gcgtagccgg ccctagtccg atggcggcga cccgccgcgc ccggctacgc cgcgcttgcg
     6541 atcgtcacta gtccagctcc cacaccgcgg tcaccgagaa gctcacggtc tgctggccgg
     6601 gctccagcgg gaccgcggcg atgccgcgtt gtggcaccgg cggcgccccg gccacgggtc
     6661 cgccgccggc gacctccgaa atggaaagca ccctgcccag gcgcaggccg gacaactggg
     6721 cgtactggtc ggcgcgtccc ttggcgtcgt tgaacgcccg cgcgcgggcg tccttgacca
     6781 gctgcgaatc gtcggcgatg gagtagctga ccgagctgat ccgggtggcg tcgccgccgg
     6841 tgctgacgat cagagccagc atgcgtgacg cggtgtcggt ggggtgaatc ttgacctgga
     6901 tcgcgttggt ggcacggtag ccggtgatgg tggcggtccc gttgggctcg ggattgccgt
     6961 actgcggctg cagcgtgacc gtggtggtgg cgatgtcctt gcggtccagg cccgcgccga
     7021 ccagcgcgtt gatcacggct tgctggcgat cgttggtctg gttcatcgcg gtgctgacgt
     7081 cgggagcggt gaactcgatg ccggcgtcgg cggtcagggt gtcggggacg ccttggacct
     7141 gccccgagcc gacgacggtc acttgacgcg gattcgccgc cggcgatgcc ggattgtgct
     7201 tgacgcaccc cgacagcgtg gcgacggcgg ccaagccggc cgcagccacc gcaatcgatg
     7261 tgcggaccaa cctcggggcg ttcgttccga tcggcatgtg tggacactag cgcgaccgac
     7321 cgggtgcgtc ggggacccag tcggctccac tcgcccggcg gcagctgggg tgacttggag
     7381 tgtgacccgg ccgagctgcg ggcgcgcgtt cttgccgagg tcggtgagga atacgcgccg
     7441 tcgtcgagcc agatcgcgga cttccaggaa ttcgtccgcg ccgttcgctc ggcgtggggc
     7501 agcggcgctt cggcgaagtg gatgtcccag atggccccat cgatccgcgg gacacggcag
     7561 gccgggatat tggagcgcat gtgcgcaagc ccggggatga ttcgggcgac gcttgaatcg
     7621 aacttccggc tcgacgtgcg ggggattttg ccgacgctct ccgtgcccac cctggtcatc
     7681 catgcccgcg acgacctcat gccggtgcag tgcggccgat tcctcgccgc caggattccc
     7741 ggcagcgcga tacctcgagg tcgacggcgc ggctcacgcg ccctggttga ccgagcccga
     7801 cacgatcctg accgtcatcg aggagttttt gaccggcagc catggtgcac cgtcgcagtc
     7861 ccatcgcgcc ctgcgcaccg tcttgttcac cgatatcgtc gcctcgacgc agcacgccgc
     7921 agcgacaggc gatgagcggt ggcgcgcggt gctgcaccgc ttcggggagg tcaccgccga
     7981 cctcaccgaa cgcttcggcg gcgcggtcgt caagagcacc ggcgacggtc acctggccac
     8041 gttcgacggg ccgacgcagg ccattcgctg cgccgaggcg ttgcgcggtg acgccgagac
     8101 cctgggcatc cagatccgag tcggcatcca caccggggag tgcgaactga tggacggcga
     8161 catcgggggg ctggcagtcc acatcgcggc gcggatcatg ggtcaggccg gggccggtga
     8221 aatcctcgtg tcccgaaccg tgtctgacct cgtggtcggg tcgggcacgg gtttcgagga
     8281 tcgcggcggc gttgagttgc gtggcgtgcc cggcatctgg cagctcctgg cggtcgatcg
     8341 ccacggccca cgggcgggat cggcagaggc cgtgctggcg gcaacgccga ccccgcaccg
     8401 cgggaccgcg atgcgccgct cggatcgcgc cgtggaggtg atcgccaggc ggacgccatg
     8461 gatcgtgcgc gggatggccc gcctcgcccc ggccaacggt cgcagatgaa cagcgggatc
     8521 agacgtgtgc gacgatcgcc gcgacggccg tccagacaac cagaacccag atcgcaattg
     8581 cgatggcccc ggagatcacc gccatcgtgt ccagtttcgc tttcatgatg tcctcctcgt
     8641 ccgcggccgt atcccgcgca gtgcaacctc gcagcgcgat cgctagctgc gcaacagccg
     8701 cagcttagac cggcaatctg agggtgtcgc ttccgagact gcccgtgccc tcagccaact
     8761 gcttatcacg ccgtccgccg ccagatctcc gctcatcctg ccgaggcggg cagcaatccg
     8821 ggcgcatcgg ttacccatcc aacaatgctg gcatcggggt cggcggtgag gcatccaact
     8881 ggagcctaag tcgtaacgag gatcttgcag tgtcgctcgg ggtcggcgag gtcgtcgaag
     8941 gcctggccga ccccctccag gcccgcttct ccggtgatca gcggggcgac gtcgatctcg
     9001 ccttcggcca gcgcacgcag cgagtcggcg aattcgtccg ggctgtaggc gaatacgaac
     9061 tgcacgttga tctctttggc gatcgcgaaa aatggatgca cggtgtcggg ctgcatgcac
     9121 accccggcaa ccaccagacg ggtgcccggc cgcgcccgca gcaacacgtc gtcgatgatt
     9181 ccaggcactc cgaccgcctc gaacacgacc gcggccttga cggtgtcgaa cggtgagccc
     9241 tgcgccgcat ccagcgtccg gtgggcaccc atcgtggcgg cgagctcccg ccgtataggc
     9301 gaaaagtcgg atgccacaat ggtttcgatg cctcgcgcgc gaagtgctgc gatgatggcg
     9361 atgccgatcg gtccgcaacc gagcaccagg gcggtctcgt tcggcgcgat gtccgatttg
     9421 ttgacggcgt gcaaccccac cgccatgggt tcggtcaacg cggcgtgttt gaggtcaagc
     9481 ccgttgggga tgggcaagag tagcggcgcc gagagcagca tccgctcggc gtagccgctg
     9541 agcgtgctgt tgctgtagcc gatcggctgg acgcccttgg cggacagcaa cactggaatc
     9601 gacgtgaccg gggttccggg tggatgcgtt tcggtgtcgg gcccggcttc gagcacctcg
     9661 gcgctgaatt cgtgacccat gaagacgtcg ctggtcatat cgaggttcat tcccgaggcg
     9721 gcaccaccgc ccaaccgatc gcccagcgcc aacacctccg cgccgtgggc ggcgaaatgc
     9781 aaatcggaac cgcagattcc acacgctcgc acggcaacga gcacctggcc gggttcgggt
     9841 accggctcgg gcacgtcgtc ccgatagacc atccggccgt ctcgtaatac cgcagcgcgc
     9901 atcagtttcc cacgcttttc tgttcgtcga cgtgctcggt gatggctttc accaccgctt
     9961 cgccggcgcg gccgatcagc tggtcgcgtt tgcctctggc ccagtcgtgc gacgatcgcg
    10021 ctgcttcgag ctgtcccttg aaatgtggaa tcaccttgcg ggccatcagg tcatagcagt
    10081 ggtaagtcgc ttccggcgaa gcccagtcgt gaccgagcat gagcagcgtc ccgaagccgc
    10141 ctgaccgttc gagcagatct tcaatgtgcg cgatcgcgtc ctcaggcgtc ccgatgcagc
    10201 aattcccttt ggccgcatag tcttcgacga attcttgcgg tgactgggcg ccttctacgg
    10261 tgttggccag cgggacgaat ccggccgcgc cgaagtagtt ggcgaaatcc tgcaatccgt
    10321 aggtgcagtc gtcgatcgcc tgctcacggc tgtccccaat atgcatgatg ctcaagacac
    10381 gccagttcac ccggtccggc tcgtcgcggc caactttggc cgcctgcgcg cgcaccacct
    10441 cccacgtgtt ctccagcgcg gcatagccgc cgggcaccga catcgacagt gacaggagcg
    10501 acgtgccaag tgcaccggcc aggcgcggtc cggacggtga aatcattgcc gccgcggcta
    10561 tctcggggta gggccaggtg taggggcgga tgtgcaactg cgcgtcgcgc aaggtgaacc
    10621 agtcggagtg gcggtcgatg cgctcagtgg gtccggcgcg gaacagcgca aggatcgcct
    10681 cgagcgattc ctgcatcatc cgccgctgct cgactgggtc gatgcccatc atgtacgcgt
    10741 ccgacgggag tgcgccggga ccggtaccga acatcacccg gccgcgcgtc aggtggtcca
    10801 acagcaccca gcggtcggcc accatcagtg ggtggtggta gggcagcgag accactcccg
    10861 tgcccagccg gatgtgcttc gtccgctccg ccgcggccgc gatgaacact tccgggcagg
    10921 cgatcagctc gtagccaccg gagtggtgtt cgccgaacca ggcttcgtcg aatccgagac
    10981 ggtccagcgc gacgacgcgt tccatgtcgt attccagtgc caccgtgggg gattggccaa
    11041 cgggatgaaa cggtgtgatg aagaccccga agcgtagtgg cgcgctcatc gaagctccaa
    11101 tcccttgagg aactccagca gggtcgcgtt cacctcgtcg ggccgttcct gttgtaacca
    11161 gtggcccgcg ccgtcgatca tcacctggcg gtacggtccg gaaatcacct ccaaagcccg
    11221 gtcggtgcgg gtgaacgcca gcaccggatc ggccgtgccg gcgataaaca acgacggcac
    11281 agcgattttg gcgccggtca gttcggcggt ggtctcccag ttgcgatcga aattgcgata
    11341 ccagttcagg ccgccggtga atccggtgcg ggcgaactcg ctgatgtaat ggtcgagttc
    11401 gtcctggctg agccaatccg gcaaccgttc tggctcggga agccggtcga ggaagccctc
    11461 cggccccggg gccaccatcc gcatccccgc gctgtggtcg ccggataccg tcaacccacc
    11521 catcatccgg cgcatcgtac gtgcgggatc ggcgtcgagt tcggcgtcgg caacgccggg
    11581 ctcctggaaa tagaggatgt agaagaagtt ctcgccaaag gtggcgcgga aggcctgtgt
    11641 cgggggcacc tttgcgcggg gcaccaccgg gacgctcaac gcggcgacgg ccgcgaccct
    11701 gtcggggtgc agcagcggag cgttccacac cacggcggca ccccagtcgt gaccgatcca
    11761 gaccgcacgc tcggcaccga cgtcgtcgag gaggccgacc acatcggcgg tcaactcgcg
    11821 aatgttgtag gcctcgatcg catccggacg agatgagccg ccgtagccgc gctgatcggg
    11881 cgccagcacg tggtagcccg cctcggcgag ggcgggtatc tgatggcgcc acgaatacgc
    11941 cagctccgga aagccgtggg ccaggaccac cacgggtgcg ccgcgatctc ccgcttcggt
    12001 cacccgcagc tgcacaccgt tggtatctac taaccgttcg gttgatatgg gcacgatctc
    12061 accaaatcac aacgatcccg ggctgagaag ggctgaccgc ctggtagata tcggggttgg
    12121 ggcatcacag gaaagcaggt gactcgcgtg aggcggctag accatatcgt cttatggaca
    12181 cacaatctgc aggcttcgat ggacttctac accagagttg tcgggctctc gccggtacgg
    12241 ttcgacgagt tcgccgccgg ggaagcgccg ttcccgagtg tgcgtgtctg cgaggactcg
    12301 atcatcgacc tgtcgccggt ggacaacagt gccggcaccg agtcgctgac cggggtcggc
    12361 ggaagtgccg ggcatcaggt caatcacgtc tgcatcgcgt tctcgaagtc ggagtacgac
    12421 gcactggacc agcgtctgcg ggcagaaggc gtggacacca gcgcgcgcct gagccctagc
    12481 ttcggggccc gcggatgggc gccccagggt tattacttct ctgatccgga cggcaacgtg
    12541 gtcgaggctc gctattacga ctaacggctg aacgtggccc ggcgacccgc gtggtgcgcc
    12601 aagcccaggg ccatgtcgat gcctcaccgg gaactcccgg tcgttgtctg ggcagcaagg
    12661 tggtgacccg ctgcgccccg gctctgccgg agctggcgat caccactagc ggtagcctgg
    12721 acttttccag cttcgcctat cggggaagga cctggccggc aaggccgatg attgatgaac
    12781 cggaaaaacg tgattcgcac gctcactgcg atcgctgtga tcgtgctgct cggctggtcg
    12841 ttcttttact ttagcgacga cacccgcgga tacaagcccg tcgacacctc ggtggcgatg
    12901 acccaaatca acagcgacaa cgtcgcgagc gcgcagatcg acgatcgcga gcagcagctg
    12961 cggctgacgc tgaagaaggc caacggcgac accgacaact ctgacaaggt catcaccaag
    13021 taccccaccg gctacgccgt cgacctgctc aacgcgctca ccgccaagaa cgccaaagtc
    13081 agcacaaccg tcaacgaggg cagcatcctg ggcgagctgc tggtctacgt gctgccgctg
    13141 ctgttgctgg tgggcctgtt cgtgatgttc tcccggatgc agggcggcgc ccggatgggc
    13201 ttcgggttcg gcaagtcgcg ggccaagcag ctctccaaag acatgcccaa gaccacgttc
    13261 gccgacgtcg ccggcgtcga cgaggccgtc gaggagcttt acgagatcaa ggacttcctg
    13321 cagaacccgg cgcggtacca ggcactgggc gccaaaatcc ccaagggcgt gctgctctac
    13381 gggccgccgg ggacgggcaa gacgttgctg gcccgcgccg tggctggcga ggctggggtg
    13441 ccgtttttca ccatctccgg ctccgacttc gtcgagatgt tcgtcggtgt cggcgcctcg
    13501 cgagtgcgcg acctgttcga gcaggccaag cagaacagcc cgtgcatcat cttcgtcgac
    13561 gagatcgacg cggtgggccg ccagcgcggc gccgggctgg gcggcggcca cgacgagcgc
    13621 gagcagacac tcaaccagtt gctggtggag atggacggtt tcggcgagcg cgccggcgtc
    13681 atcctgatcg ccgccaccaa ccggcccgac atcctggacc ccgcgctatt gcgccccggc
    13741 cgtttcgacc ggcagatccc ggtgtccaac ccggacctgg ccggtcgccg cgcggtgctg
    13801 cgagtgcatt ccaagggcaa gccgatcgct cccgacgccg acttggacgg gctggccaag
    13861 cgcaccgtcg gcatgaccgg cgccgacctg gccaacgtca tcaacgaggc ggcgctgctg
    13921 accgcccgcg agaacggcac cgtcatcacc ggccccgcgt tggaggaggc ggtggaccgg
    13981 gtgatcggcg gcccgcgccg caagggccgg atcatcagcg agcaggaaaa gaagatcacc
    14041 gcctaccacg agggcgggca cacgctggcg gcctgggcga tgccggacat cgaccccgtc
    14101 tacaaggtga cgatcctcgc ccggggacgc accggtggcc acgccgtcgc ggtgcctgag
    14161 gaagacaagg ggctacgcac ccgctcggag atgatcgcgc agctggtgtt cgcgatggga
    14221 ggtcgcgccg ccgaggaact ggtgttccgc gagccgacca ccggtgctgt gtcggacatc
    14281 gagcaggcca ccaaaatcgc ccgctcgatg gtcaccgaat tcggcatgag ctccaagctc
    14341 ggcgccgtca agtacggcac cgaacacggc gacccgttcc tcggccgcac catgggcacg
    14401 caggccgact actcccacga agtcgcgcgc gacatcgacg acgaggtccg caagctcatc
    14461 gaggcggcgc ataccgaagc gtgggagatc ctcaccgaat accgcgacgt gctcgacgcc
    14521 ctggcgggcg agctgctgga aaaggaaacc ctgcaccggc ccgagctgca agcgatcttc
    14581 ggcgacgtcg aaaagcgccc ccggctaacc atgttcgacg acttcggtgg ccgcatcccg
    14641 tcggacaaac cgccgatcaa gacgcccggc gagcttgcca tcgagcgtgg cgagccgtgg
    14701 cccccgccgg tcgtcgagcc ggccttcaag gcggccatcg cccaagccag ccaggccgtc
    14761 gaggccgggc aaacggacgc cgaccgaaac gctcatggcg gcaacggttt tcacgatggc
    14821 cacggggcgg gtaatcgcca ggccacgcca cctggtgcta cccagcccga ctacggtgcc
    14881 ccggccggct ggtatgcgcc aggatggccg ccacagcaac aacaaccgaa ttcttggcat
    14941 ccgcccgcgc agtctccgca gcagccctat tggccgcagc ccgccgggag ctatccgggg
    15001 tacccgggcc cgcacgttca gcccggccag cacggtcagg cccacccctc gtaccccgcc
    15061 tacccgccct atgcagcgcc cggtccgtcc gacgcggacg ccggaaagtc ccctgaccag
    15121 ccggatgacg gggtgagccg gtccaacccg ccggcccacg gctgacgaac ggaggattcg
    15181 atggctctcc cggacttggg gccggatctg gggccggact cccgcaccgc gcccttccgc
    15241 atacggacgt tcgaccagca acgtgccgag gccgcggtcc gcgagttgct gttggcgatc
    15301 ggcgaagacc cggaaagaga cggtctgcag gacacgccgg cccgcgtcgc ccgggcctac
    15361 cgcgagatgt tcgccgggct ctacaccgac ccagacacgg tgctgaacac catgttcgac
    15421 gaagaacacg acgagctggt gatcgtcaag gagatcccgc tgtactccac ttgcgaacat
    15481 cacctggtgt cgttccacgg cgtggcccac gtcggctaca tccccggtga cgagggcaga
    15541 gtgaccggcc tgtcgaagat cgcgcgcctg gtcgaccttt acgccaagcg gccgcaggtg
    15601 caggagcggc tcaccagcca gatcgccgat gccctggtga agaagctcga tccccgcggg
    15661 gcgatcgtcg tcatcgaggc cgagcacctc tgcatggcga tgcgcggagt gcgcaagccc
    15721 ggtgccgtca cgacgacgtc cgcggtgcgt gggctgttca aaaccaacgc cgcatctcgg
    15781 gccgaagcgc tcgacctcat cctgcataag tgagtccggc gcccgtgcag gttatgggag
    15841 ttctgaacat cacgcacgat tcattctcgg acggtggccg ctacctggat gccgacgacg
    15901 ccgtcgcgca tggtctggca atggcggccg aaggcgccga gatcgtcgac gtcggaggcg
    15961 agtcgacccg tcccggcgcc acccgagtcg atcctcgggt tgaggcgtct cgcgtgttgc
    16021 ctgtcgtcaa agaacttgcg gcacagggta ttaccgtgag catcgacacg atgcacgccg
    16081 aggtggcgcg cgcggcgctg cagagcggag cgcggatcgt caacgacgtg tcgggtgggc
    16141 gagccgatcc cgcgatggca ccgctgctgg ccgaagccgg cgtgccctgg gtgttgatgc
    16201 actggcggcc cgtgtcgttc gaccgcccgc atgaggcccc gcattaccgc gacgtggtgg
    16261 ccgaggtgcg cgccgagctg ctcgccgccg tcgacgacgc ggtggccgcg ggtgtggatc
    16321 ccgcccagct ggtgatcgat cccggactcg ggttcgccaa gacgggacaa cacaattggg
    16381 cgctgctgca tgcgttaccg cagctggtcg cgaccgggat tccggtcctg ctgggtgcct
    16441 cgcgcaagcg cttcctgggt acgttgttgg ccgcgccgga cgggtcgcca cgaccgcccg
    16501 acgggcgcga gacagcgacc gcgacgattt cggcgctggc cgcgctgcac ggggcctggg
    16561 gtgtgcgcgt gcacgacgtg cgggcctcgg tcgatgcgct caaggtcgtc ggggcctgga
    16621 cgggatgcga tggctgatcg aatcgagttg cgcggcttga caattcgggg caggcatgga
    16681 gtcttcgatc acgagcgcgc caacgggcag gacttcgtcg tcgatatcac cgtctggatc
    16741 gacctcgccg atgccggcgc cagcgacgat ctggcggaca cctacgacta cgccgcgctg
    16801 gcccagctgg cggccgacgt cgtcgcgggc ccgccgcgga acctgatcga agccgtgggc
    16861 ggcgacatcg ccgaccaggt gatggccgac caccgcgtgc acgccgtcga ggtggttttg
    16921 cacaagccgc gggctcccat tccgcatcag ttcgccgacg tcgcggtggt ggttcggcgg
    16981 tcgcggcgcg gcggtcgtgg ttcggtgatc cccgccggcg gggcgttatg agccgcgacg
    17041 accacgatga tgccggcccg gatggttttc gggccggcat tgagacgtgg cgcccatgag
    17101 ccgcgtggtg ctgtccgtcg gctccaatct cggcgatcgg ctggcacggc tgcagtcggt
    17161 cgtcgacggc ctcgagggcg agctggtagc tgtatcccct gtctacgaga ccgacccctg
    17221 gggcc
//
LOCUS       DASSGQ010000292         3263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_294992,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000292 DASSGQ010000000
VERSION     DASSGQ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294992"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="VFI55_12980"
     CDS             <1..794
                     /locus_tag="VFI55_12980"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7074983.1"
                     /translation="STNWTDLAGLLSPRLAGEALDLPGFGRSFPPPDDDYSLRSHVRA
                     VIEYLEYVKRGPVHLFGNSMGGAIAIVVAASRPDLVRTLTLVSPAVPDLRPRWRRQDP
                     VLPLMMLPGLGTALLRRLEQGSPEIRVRRMLRLCFADPGSVPPERLTEAVAEIRYRAR
                     LGWASDAVIRSLRGLVASYLAPGASRMWARMGAIKAPTLVVWGDRDRLVNVTRAARTA
                     RAIPGARLLVIRDVGHVAQMEQPGTVARAVLALLDDTPDVLSTRP"
     gene            857..1879
                     /locus_tag="VFI55_12985"
     CDS             857..1879
                     /locus_tag="VFI55_12985"
                     /inference="COORDINATES: protein motif:HMM:NF022794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3152 domain-containing protein"
                     /protein_id="HET7074984.1"
                     /translation="MPEPTRPRLATLPREDWVNGPRAGRAIRRSASSYDAGRPSSSYD
                     AGPSGERSPGRLRRFVREYGWRAYALPLLIVITIVALMRMNEPGGPPRPASAAGASGA
                     SLAGAGPPRAAPTDTPMMPDKAGSAVQREVLASDALPPGGPYTQRGKGTFRVVPGTGP
                     VVGTGALHRYTIDIEDGVTGINPATFATTVQAALDNPQSWTGHGRGVALQRVDSGPVE
                     FHITLTSAMTVRALCGYELKIETSCYAAAHDQRVVLNVARWIRGDMAYVGDLAIYHLY
                     MVNHESGHALGHSHAHACLKNGFAPVMMQQTISLKAANGKTCQANPWPYPPGASDAPG
                     VEQPGT"
     gene            1971..3149
                     /gene="moeZ"
                     /locus_tag="VFI55_12990"
     CDS             1971..3149
                     /gene="moeZ"
                     /locus_tag="VFI55_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005203671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/sulfurtransferase MoeZ"
                     /protein_id="HET7074985.1"
                     /translation="MSLPPLVEPAESLSVDEVRRYSRHLIIPDVAMAGQKRLKNAKVL
                     CVGAGGLGSPALMYLAAAGVGTLGIVEFDVVDESNLQRQIIHGQSDVGRSKGESARDS
                     VREINPFVTVNLYEERLDSSNVMELFAQYDLIIDGTDNFATRYLVNDACVLLHKPYVW
                     GSIYRFDGQAAVFWADEGPCYRCLYPEPPPPGMVPSCAEGGVLGVLCASIGSIQVNEA
                     IKLITGIGEPLVGSLMVYDALEMEYRKITVRKDPNCAVCGDHPTVTELIDYEEFCGVV
                     SEEAQQAAAGSTITASELKDLIDADKPIFLVDVREPAEWEIVRIPGATLVPKDEILRG
                     DALAMLPQDKQIVMYCKTGVRSAETLAAVKSAGFRDAVHVQGGVIAWVNQIDPSLPSY
                     "
BASE COUNT          517 a         1049 c         1109 g          588 t
ORIGIN      
        1 cgtcgacgaa ctggaccgat ctagccgggt tgttgtcgcc ccggctcgct ggtgaggcgc
       61 tggacttgcc cggcttcggc cgatcgttcc caccaccgga cgacgactat tcactgcgct
      121 cgcatgttcg ggcggtgatc gagtacctgg aatacgtcaa gcgcggcccg gtccacctat
      181 tcggcaactc gatgggtggc gccatcgcga tcgtcgtcgc ggcaagccgc cctgacctgg
      241 tgcggacctt gacgctcgtt tcgccagcgg tgccggatct tcggccgcgc tggcgacgcc
      301 aagatccggt gttgccgctc atgatgctgc ccggtttggg gactgctctg cttcggcggc
      361 tcgaacaggg gagtcccgag atcagagtgc gccgaatgct tcggttgtgt ttcgctgatc
      421 cagggtccgt gccgcccgaa cgtctgaccg aagcggtggc cgagattcgt taccgcgctc
      481 ggttggggtg ggcgagcgac gctgtcattc ggtccttacg cggcctggtg gcctcgtatc
      541 tcgcgccggg tgccagccgc atgtgggcgc gcatgggcgc gatcaaggcg ccgacgctcg
      601 tggtctgggg tgatcgggat cggttggtga atgtcactcg cgccgcccgc accgcgaggg
      661 ccattccggg tgcgcggctg ctggtgatcc gcgacgtcgg acacgtcgcc cagatggaac
      721 agccggggac ggtggctcgc gcagtgctcg cccttctcga cgacacgccg gacgtgctca
      781 gcacccggcc gtgacatggc ggcgcgcccg ctgacacgat gacgccaggc tcgaccggct
      841 gtgggaggct ttgtcggtgc cggaaccgac ccgaccacgc ttggcgaccc tgccccgcga
      901 ggattgggtg aacggccccc gcgccggtcg tgccatccgg cgttccgcca gctcgtacga
      961 tgcgggccgg cccagcagct cctacgatgc cggcccgtcc ggcgaacgct cgccgggtcg
     1021 tctgcgccgt tttgtgcgcg agtacggatg gcgcgcatac gcgctaccgc tgctgatcgt
     1081 catcacgatc gtcgccctca tgcggatgaa cgagccgggc gggccgcccc gtcccgcttc
     1141 cgccgctggg gcctcgggtg cctcgctagc cggtgccggg ccgccgcggg cagcaccgac
     1201 cgataccccg atgatgccgg acaaggccgg ctcggccgtg caacgtgaag tgctggcctc
     1261 cgacgctcta cccccgggcg gcccttatac ccagcggggt aagggcacct tccgcgtcgt
     1321 cccaggcacg ggtccggtcg ttgggaccgg cgccctgcac cgctacacga tcgacatcga
     1381 agacggcgtg accggtatca atccagctac cttcgcgacg accgtgcagg ccgcgttgga
     1441 caacccgcag agctggaccg gtcacggcag gggggtggca ttgcagcggg tcgacagcgg
     1501 tccggtggag tttcacatca cgctgacgtc ggccatgact gttcgggcct tgtgcggcta
     1561 cgagctgaag atcgagacgt cctgctatgc ggcggcacat gaccagcggg tggtgctgaa
     1621 cgtcgcccgc tggattcgcg gtgatatggc ctacgtgggc gatctcgcca tataccacct
     1681 gtacatggtc aaccacgagt ctgggcatgc gctcggccat agtcacgccc acgcctgcct
     1741 caagaacggg tttgcgccgg tcatgatgca gcagacgatc agcctcaaag cggcgaacgg
     1801 caagacctgc caagccaatc cgtggcccta cccgccgggc gcctcggatg cccctggcgt
     1861 cgagcagccc ggcacctgat cgatcgtctc gtgcggtcgc ccgtccggaa tgacgctggg
     1921 atgatcgggg ttatccaagg ttgagtaccc gccgaaagga aacccacgtc gtgtcgttgc
     1981 caccgctcgt ggagccggcc gagtccctat ctgtggacga ggttcgccga tacagccgcc
     2041 acttgatcat tccggacgtt gccatggccg ggcagaagcg gctcaagaac gccaaggtgc
     2101 tgtgcgtcgg cgccggcggg ctcgggtcgc ccgcgctgat gtacctggcc gccgccggtg
     2161 tcggcacctt gggcatcgtc gaattcgatg tggtcgacga gtcgaacctg cagcggcaga
     2221 tcatccacgg tcaaagtgat gtcggccggt cgaagggcga gtcggcccgg gattcggtgc
     2281 gcgaaatcaa cccgttcgtg accgtgaacc tctacgaaga acgcctcgac tcctcgaacg
     2341 tcatggagtt gttcgcacag tacgacctga tcatcgacgg aaccgacaac ttcgctacgc
     2401 gctacttggt caacgacgcc tgcgtcctgc tccacaagcc gtacgtgtgg gggtcgatct
     2461 accgcttcga cggtcaagcg gccgtgttct gggccgatga gggcccgtgc tatcgctgcc
     2521 tgtacccgga gccgccgccg cccggcatgg tgccgtcgtg cgccgagggt ggtgtcctcg
     2581 gtgtgctgtg cgcgtcgatc gggtccatcc aggtcaacga ggcaatcaag ctgatcaccg
     2641 ggatcggtga gcctttggtc ggctcgctca tggtgtacga cgcgctggaa atggaatacc
     2701 gcaagatcac cgtccgcaag gacccgaact gcgcggtatg cggagaccac ccgacggtga
     2761 cggagctcat cgactacgag gagttctgcg gcgtcgtgag cgaggaggcg cagcaggccg
     2821 ccgccggatc cactatcacc gcgtcggaac tgaaggatct catcgacgcc gacaagccga
     2881 tcttcctggt tgatgtgcgc gagccggcgg agtgggagat cgtgcggatt ccaggcgcga
     2941 cgctcgtccc caaggatgag atcttgcgtg gcgatgccct cgccatgctg ccgcaggaca
     3001 aacagatcgt gatgtattgc aagaccgggg tgcgctcggc tgagactttg gcagcagtga
     3061 agtcggccgg cttccgcgat gccgtgcacg tacagggcgg ggtcatcgcc tgggtgaatc
     3121 agatcgatcc gagcctgccc tcgtactgat agggctaccg gcgcagccct tgggggggta
     3181 tgactgcgaa atggctgatc ctgtcgcgtt gatgttgcaa ggcttgagtg agttcggcga
     3241 tcggctcgat cgcgtcggga acg
//
LOCUS       DASSGQ010000293         4255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_690014,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000293 DASSGQ010000000
VERSION     DASSGQ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690014"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            421..873
                     /locus_tag="VFI55_12995"
     CDS             421..873
                     /locus_tag="VFI55_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HET7074986.1"
                     /translation="MAQPPPPPVGHLRHRSVPFALGAPLLVAASTSLVCAVAWASDPT
                     TPGGPLPVCPTKALLGIDCPGCGSARMLYSLLHGDVVAAARFNALALVALVLLVWAYV
                     TWTYGRLTGRRIRGWQHRRWSALVTLLLVGAWFLVRNIPFAPFDALYV"
     gene            909..2408
                     /locus_tag="VFI55_13000"
     CDS             909..2408
                     /locus_tag="VFI55_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HET7074987.1"
                     /translation="MVNKSRTIPAVLAVVALTALLTACVRRCENSQPAAGDSAAGDFA
                     TAIRNKVKTEAMMAHLSKLQDIANANNGTRAVGTPGYDASVDYVVNTLRNSGFDVQTP
                     EFSGRVSHADKPAVTVGGKTVEAGALDFTLGTPPDGVSGPLAVASSGDNLGCAASDFD
                     GLAVQGAVVLVNRGSCPFAQKEKAAAQRGAVAMIVADNVDEQQMGGTLGENNDVKIPV
                     VSVTKSIGSQLRTQPGPTTIKLNARVQSFKARNVIAQTKTGSPTDVVMAGAHLDSVPE
                     GPGINDDGSGVAAVLETAVQLGNSPQVHNAVRFGFWGAEELGLIGSHNYVESLDLDGL
                     KDIALYLNFDMLASPNPGYFTYDGDQSLPSDTRGRPVVPEGSAGIERTLVAYLKSAGK
                     TAEDTSFDGRSDYDGFTLAGIPAGGLFSGAESKKSAEEAKLWGGTANEPFDPNYHQKT
                     DTLDHVDRTSLGINGGGVAYALALYAQDVSGRNGVPAMAARTRHVIAKP"
     gene            2417..3886
                     /locus_tag="VFI55_13005"
     CDS             2417..3886
                     /locus_tag="VFI55_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET7074988.1"
                     /translation="MIRRLGSVLVLTGLVAGCSSPCRVSPSAAPDLGRGLAAKVTADR
                     MFTHLHALQDIANANHGNRAEGTPGYDASVDYVAKALRDKGFKVSTPQFDRLYNVAPG
                     NPALTVAGRTYQVDQASLLVRTPAGGLTGQPVRPTQPSGCAVGDYPAVVPKGAIAVVD
                     DAGCSVVDKQNSAVAKGAAGLIVISAPNGQGAPPKLFTPGYFKQLTVPVAIVGPVGAS
                     ALARNSAPIRLVLDAEIIKITSRNVLAQTETGSSNEVIMVGAHLDSPRGGPGINDDGT
                     GVAAVLETALQLGPLAPLNNAVRFVFWGAEEDGINGAMDYVVGMDRDQLNDIAMYLDF
                     TMLGSPNAGFFTDDGDQSGPPTPGITFADVPEGSAGIERTLAGYLSLAGKRPADMPLS
                     TRADYRPFMVAGVPVGGVTTGASQAKTTVQARLWGGQPGAPFDPNYQSPRDTVDNINR
                     EALAVMGSGAAFAVGTYAESIGGVNGVPPHDKRHRTRVP"
BASE COUNT          746 a         1338 c         1436 g          735 t
ORIGIN      
        1 ggtgcgctga catccaggag tgtattttgc gttcctcgcc ggaaacagtg cttttgctaa
       61 atgggatcgt agggctacgt cctgactatg caacacattc cgccggaaat tttctgtctg
      121 ggcatccttc ccgcggtatc cacgcaatgt tgctgcgctg cggtgattgc gcaaactctc
      181 gcggcgccat taggcaatcc gatcggcaaa ccggttaatt gccagggatt ttgacgctca
      241 caacgagcgc cgtcactcgg ggcgaatgcc gccgaatcgg cgtcccacgc ctaattgcat
      301 gcccgtcgcc cagtcggcgg aaaaatagtg gactttgctt caggcactgc cggcgccggc
      361 gccggctcag gtaaggtgac tagcgcttta ccgcagttaa ctccctcgac tggacgatcc
      421 atggcacagc cgccgcctcc gcccgtgggg cacctgcgcc accgatcggt gccgtttgct
      481 cttggtgcgc cgctgctggt ggcggcgtcc acaagtttgg tttgcgcggt ggcttgggcg
      541 agtgacccga caacgccggg tggcccgctg ccggtatgtc ccaccaaggc gctcttgggt
      601 atcgattgcc cagggtgcgg tagcgcacga atgctgtatt cgctgctgca cggcgatgtg
      661 gtggccgccg ccaggttcaa cgccctggcc ctggtggcac tcgtgttgtt ggtgtgggcg
      721 tacgtgacgt ggacctacgg gcgcctgacc ggccgacgga tcaggggctg gcagcaccgg
      781 cgatggtcgg ctctggtgac gctgttgttg gtgggcgcgt ggtttttggt gcgcaacatc
      841 ccgtttgcgc cattcgacgc gctatatgtc tgatcccagt gggtgttgat cctctaacct
      901 tgggcgcgat ggtaaacaaa tccaggacga ttccggcggt gttggccgtg gtcgccctga
      961 ccgcattact gaccgcctgt gttcgccgtt gcgagaactc gcagccggcg gccggcgact
     1021 ccgccgcggg cgatttcgcc accgcaatac gcaacaaggt caagaccgag gcgatgatgg
     1081 cgcacctgtc gaagctccag gatattgcca acgcgaataa cggcacccgt gcggtaggca
     1141 cccccggtta tgacgccagc gtcgattatg tggttaacac gttgcgcaac agtggctttg
     1201 acgtccaaac gcccgagttc tcggggcggg tgtcgcacgc cgacaagccg gccgtgaccg
     1261 tcggcggcaa gaccgtagag gcgggtgcac tggacttcac gctcggcacc ccgcccgacg
     1321 gggtgagcgg gccgctggca gtcgcgtcca gcggggacaa cctcggctgc gccgcatccg
     1381 actttgacgg ccttgcggtg caaggcgcgg tggtgctggt gaaccgcggc agttgcccgt
     1441 tcgcccagaa ggagaaagcc gcggcgcagc gcggcgctgt cgcgatgatc gtcgccgaca
     1501 acgtcgacga gcagcagatg ggcggaaccc tgggcgaaaa caacgacgtg aagatcccgg
     1561 tggtgagtgt caccaagtcg atcggatcgc agcttcgcac gcagccggga ccgacgacca
     1621 tcaagctcaa tgcgcgcgtc cagagtttca aggcgcgcaa cgtcatcgcg caaaccaaga
     1681 cggggtcgcc caccgacgtg gtgatggcgg gcgcgcatct ggacagtgtg cccgaggggc
     1741 cgggcatcaa cgacgacggg tcgggagtgg ccgcggtcct ggaaaccgct gtccagctgg
     1801 ggaattcacc tcaggtgcac aatgcggtgc ggttcggttt ctggggcgca gaggaacttg
     1861 gactgatcgg gtcgcacaac tatgtggagt cactggacct ggatgggctc aaagacattg
     1921 cgctgtacct caacttcgac atgctcgcgt cgcccaaccc cggttacttc acctacgacg
     1981 gggaccagtc gttgccatcg gacacccgcg gccggccggt ggttccggaa ggatcggccg
     2041 gcatcgagcg aaccctggtc gcgtacttga agtcggccgg aaagaccgca gaggacacct
     2101 cgttcgacgg tcgttcggac tacgacggat tcaccctggc cggcatcccg gcgggcggcc
     2161 tgttctccgg cgccgagtcc aagaagtccg ccgaggaggc caagctctgg ggcggcaccg
     2221 ccaacgagcc cttcgatccc aactatcacc agaaaacaga cacccttgac cacgtcgacc
     2281 gcacctcgct gggcatcaac gggggtgggg tcgcctacgc gctggcgttg tatgcgcagg
     2341 acgtcagcgg ccgaaatggg gttccagcca tggccgcccg tacccgtcac gtgatcgcta
     2401 agccatgatg ggaacggtga ttcggcgact gggctcggtg ttggtgctga ccgggctggt
     2461 ggcgggatgc tcgtccccgt gccgggtgtc gccgtctgcc gcgccggacc tgggtcgggg
     2521 attggccgca aaggtgaccg cggaccggat gttcacccac ctgcacgcgc tgcaggacat
     2581 cgccaacgcc aaccacggga accgggcgga gggcacgccg ggctacgacg cgagtgtcga
     2641 ttacgtggcg aaagctttgc gggacaaggg ttttaaggta tcgacgccgc agttcgaccg
     2701 actgtacaac gttgcgcccg gcaacccggc actgacggtg gccggccgca cgtatcaggt
     2761 cgaccaggcg tcgctgctgg tccggacacc ggccggcggc ctgaccgggc agccggtccg
     2821 gcccacgcaa ccgtcgggtt gcgcggtagg cgactacccg gccgtcgtac ccaagggggc
     2881 gatcgccgtc gtcgacgacg ccggttgctc ggtggtcgac aaacagaaca gcgcggtggc
     2941 caagggtgcg gccgggctga tcgtgatcag cgcgcccaat ggccagggcg ccccgccgaa
     3001 gctgttcacc ccgggttact tcaaacagct caccgtgccg gtcgccatcg tcggccccgt
     3061 cggcgccagc gcgctggcac gcaatagcgc gccgatacgt ctggtgctgg acgccgagat
     3121 catcaagatc acatcgcgaa acgtgctggc gcagaccgaa accggctcat cgaacgaggt
     3181 gatcatggtc ggtgcgcatc tggacagccc gcgcggcggt ccgggcatca acgacgatgg
     3241 caccggcgtg gccgccgtgc tggaaacggc gctgcagctg ggcccgctgg ctccgctgaa
     3301 caacgcggtg cggttcgtgt tctggggggc cgaggaggac gggatcaacg gcgccatgga
     3361 ttacgtcgtt ggcatggacc gcgatcaact caacgacatc gcgatgtacc tggacttcac
     3421 catgctgggc tcgcccaacg ccgggttctt caccgacgat ggcgaccagt ccggcccgcc
     3481 cacacccgga atcacattcg cagacgtgcc cgaaggttcg gccggcatcg agcggaccct
     3541 ggccggatat ctgagcctgg ccggtaagcg gcccgccgat atgccgctga gcaccagggc
     3601 cgattaccgc cccttcatgg tcgcgggcgt gccggtcgga ggggtgacca ccggcgcttc
     3661 gcaggccaaa accacagtgc aggccaggct gtggggcggt cagccaggtg ctccgttcga
     3721 tcccaattac cagagccccc gcgacacggt cgacaacatc aaccgggaag ccttggcggt
     3781 catgggttct ggcgcggcgt tcgcggtggg cacgtatgcg gagtcgatcg gcggcgtgaa
     3841 cggtgtcccg ccccacgaca agcgacatcg cacgcgggtg ccgtagccgc gggcagtcga
     3901 tggccacaac cggtgacccg ccgaaaaggt tgatgctcct gtcggtttca agggcgtttg
     3961 tcaggcggcg ttgagccatt tgccgtagtt ggtggctagg tgtcgttgcg ctggaatccg
     4021 cgttcgaggc gggagatgac cgagggccaa acgccgaagt ggtcggcggc cacggtcagg
     4081 gtgatgtttt tggctcggcg cacgaccatc cagccgggcc gcgttatcag attaatgtca
     4141 gtagacccat tcagactcaa atgcatgcgc gataaacggc tacacgaaca cggaggagcc
     4201 ggagcctccc ggcgcctcga cgcgcgcatc caagctccct gataccgacc agtgg
//
LOCUS       DASSGQ010000294         3704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_720065,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000294 DASSGQ010000000
VERSION     DASSGQ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720065"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..651)
                     /locus_tag="VFI55_13010"
     CDS             complement(193..651)
                     /locus_tag="VFI55_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7074989.1"
                     /translation="MTATEHAPASADTPTLLSIGELSERTRVPTSALRYYDELGLVRP
                     AARAAGRRRYSASAVRDVGLILFFREIGFSLAEIERFIGGERQSRRAMIDHKLAELAE
                     QQHRIEVARTALEHGRRCPDSEHMECPRFWSIIEGRQRGLSLEESHGRAH"
     gene            688..1359
                     /locus_tag="VFI55_13015"
     CDS             688..1359
                     /locus_tag="VFI55_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7074990.1"
                     /translation="MEIKERALDRINQWWGPRGGLAQFHRPTGAGGHVAGWIMSGRSS
                     NVARSRWAVQLLDVQPTDRVIELGCGPGVAVAALATRAIRGLVVGVDHSQVMIRQARR
                     RNRAAIKAGQVRLIHAPVESLVINDGQFDAALAVNTVGMWPEPTARLRELARLLRPGG
                     RIALVSQPRRPGATAATSQAVADELAGLLTEAGVEHLRTEMLDLDPPAACVLGRVAQA
                     NATEQ"
     gene            complement(1627..2172)
                     /locus_tag="VFI55_13020"
     CDS             complement(1627..2172)
                     /locus_tag="VFI55_13020"
                     /inference="COORDINATES: protein motif:HMM:NF025786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm1 family immunity protein"
                     /protein_id="HET7074991.1"
                     /translation="MTLVEGRRMILNALIHEKWQYAETWPEMSALISEVMEHLESEGT
                     VTHGSVRFQTPGEDARFMFSSRRHTGDGEWPDNYLVVAVNSATGYGGLIWYVTKDFPR
                     KGGIYDHIWVSDNPQPPDFDPRVVSDPCEPAFHDPRSTLPVSEVRKALEEFCRTGTGD
                     RPQSIAWVEGWMNGHRLDEQS"
     gene            complement(2169..>3704)
                     /locus_tag="VFI55_13025"
     CDS             complement(2169..>3704)
                     /locus_tag="VFI55_13025"
                     /inference="COORDINATES: protein motif:HMM:NF025784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DddA-like double-stranded DNA deaminase toxin"
                     /protein_id="HET7074992.1"
                     /translation="RALEDAQQAQQAAEQAEKNADDQARATMGVNSPTGEAGVQALPH
                     ISDEIVSQTPIVCPPLSGSRYCETRVKHHITGSIDFVLVTCPDLDQTFCPNDAVSDYI
                     ATAPVDSYHEQTVQLDRSDVLNIARHLVDALISDYVTCAKGITITDGKIDGSQAKEWG
                     IACAWVAADIALPAAAGAVSRAIKALRISMRTGVGLIEAYDALRAAGVSAATLSKIEG
                     DLYRAVISLCGGHSFAADTPVLLAGGDTKPISQVTVGDRVQTTDPTTGDAVTGPVSRQ
                     FVHQDTQLADLTVRDADGDTTVVHTTPGHPFWVDGAASGWVDAEDLHAGDRLRAYGAG
                     AATVVSVRTFTGTETMYDLTVDEVHTYYVEAGDTPVLVHNATCPLFVKQAWEALAGKR
                     LTTGRLFDTTGRELTAELTSGTDETTAAIDDFLRNSPDIDNPPAGPFAAATHTDTKFA
                     WWMRNNGVKDADLVINNPRGPCSGPYSCQAAIRAILKQGSTLRIWYPGATEPVVVVGV
                     AAP"
BASE COUNT          568 a         1220 c         1252 g          664 t
ORIGIN      
        1 gcctcgcatc tcaccgtcgg gtctgtcggc tggcgtgccg ggcgacgccg cgggcgacct
       61 gaatcgcgag gtgttttggc tatgaggttg tcgacggcgg caaggggagc tgatctggct
      121 acgacccgtc cagagttctg aggcatcgct caagctcgcc gcgggtccgc gttcgcagca
      181 atgcgggact tgttagtgcg ctcgcccgtg gctttcttcg agtgagaggc cgcgctgtcg
      241 cccctcgatg atcgaccaga atcgggggca ttccatgtgc tcgctgtccg ggcatcgccg
      301 gccgtgctcg agtgcggtac gggccacctc gatacggtgc tgctgctcgg ccaactcggc
      361 caacttgtga tcgatcatcg cccgtcggga ttgccgctcg cctccgatga aacgctcgat
      421 ctcagccagc gagaacccga tctcgcggaa gaagaggatc aagccgacgt ccctgacggc
      481 cgacgcggaa tagcgccgtc gtcccgcggc ccgggccgcc ggtcgcacca atccgagttc
      541 gtcgtagtac cgcagcgcgc tcgtcggcac gcgggtgcgt tcggacagct cgccaatgga
      601 caacaacgtt ggcgtgtccg ccgaagccgg ggcgtgctcg gtggcagtca ttgacttcaa
      661 gtgtactcga agtgatttgc tcccctcatg gagatcaagg agagggcgct ggaccgcatc
      721 aaccaatggt ggggccctcg cggggggctg gcacagttcc accgcccgac cggagccgga
      781 gggcacgtcg cagggtggat catgagcggg cgctcctcga acgttgcgcg cagccgctgg
      841 gcggtgcagc ttctcgacgt ccagccgacc gaccgcgtca tcgaactcgg ttgtggtccg
      901 ggcgtggccg tcgccgccct cgccacccga gcgatccggg gattggtggt cggcgtcgac
      961 cattcgcagg taatgatccg ccaggcccgc cgccgcaaca gggccgcgat caaggctggg
     1021 caggtacgcc tgattcacgc gccggttgag agcctggtca tcaacgatgg acaattcgat
     1081 gccgcgcttg cagtcaacac ggtcggcatg tggcccgagc caaccgcccg gctacgcgaa
     1141 ctcgcccggc tgctgcgtcc cggcggccgc atcgctctag tatcccagcc gcgccgtccc
     1201 ggtgccacgg cagccacctc gcaggcggtc gccgacgagc tcgcaggtct gcttaccgaa
     1261 gctggggtcg agcaccttcg aaccgaaatg ctcgacctcg acccgccggc agcgtgtgtc
     1321 ctcggacgcg tcgcgcaggc caatgccacc gagcaatagc gcgacgctgg cgacgcctac
     1381 gcgggagaat catccaaggg caatcccgcc atctacgccg aacgccctgt caccgggata
     1441 cgtcgtccgt aatgatgcgc tgagcccgac cgacctctcg gggctgcata gttggatggc
     1501 catccggcct gcagcacgct tgatcgaccc gctccttcat ggatgagctg gtacgtttcc
     1561 cgctccgcta cgcagtcttg gccctgaaaa gccggacaat ccccagatgc acctgaacgg
     1621 tgccggctag gattgctcgt ccaggcggtg cccgttcatc cagccttcga cccaggcgat
     1681 cgactggggg cggtcgccgg tgccggtgcg gcagaactcc tccagggcct tgcgaacctc
     1741 ggacacggga agggtactgc gcggatcgtg gaatgccggt tcgcacggat ccgacacgac
     1801 gcgggggtcg aagtcgggcg gctgcggatt gtcggacacc cagatgtggt cgtagatgcc
     1861 gccctttcgg gggaagtcct tcgtcacgta ccaaatcagg ccgccgtagc cggtggcgga
     1921 gttcacggcg acgacgaggt agttgtcggg ccactcgccg tcgccggtgt ggcgccggct
     1981 ggaaaacatg aaccgtgcgt cttctccggg tgtctggaaa cgcaccgagc cgtgggtcac
     2041 ggtcccctcc gactcaagat gctccatcac ctcggagatc aatgcagaca tctccggcca
     2101 ggtctcggcg tactgccact tctcgtggat cagcgcgttg aggatcatgc gtcgtccttc
     2161 cacgagggtc acggcgccgc cacaccgacg accaccacgg gctcggtcgc ccccggatac
     2221 cagatccgga gggtggatcc ctgcttgagg atcgccctga tggcggcctg acaactgtac
     2281 ggcccggagc aaggccctcg cgggttgttg atgacgaggt cggcgtcctt gaccccgttg
     2341 ttacgcatcc accacgcgaa cttcgtgtcg gtgtgggtgg ccgccgcgaa cgggccggcc
     2401 ggcggattgt caatgtcggg cgaattccga aggaagtcgt cgatggctgc ggtcgtctca
     2461 tccgtgccgc tcgtcagctc ggcggtcagc tccctgcccg tcgtatcgaa gagcctccct
     2521 gtggtgagtc gcttgccggc gagcgcttcc catgcctgct tgacgaagag cgggcaggtg
     2581 gcgttgtgga ccaggaccgg cgtgtcgccg gcttccacgt agtaggtgtg aacctcgtcg
     2641 acggtgaggt cgtacatcgt ttccgtaccg gtgaacgtgc gtacggaaac caccgtcgct
     2701 gccccggcgc cgtaggcgcg cagccggtcg ccggcgtgca ggtcctcagc gtcgacccag
     2761 ccggaggccg cgccgtcgac ccagaatggg tggcccggcg tggtatggac gacggtggtg
     2821 tcgccgtccg cgtcccgcac cgtcagatcg gcgagctggg tgtcctggtg cacgaactgg
     2881 cgtgacaccg gcccggtcac ggcatcgccg gttgtcgggt cggtggtctg cacccgatcg
     2941 ccgactgtca cctggctgat cggcttcgtg tcgccgccgg ccaacagcac cggcgtgtcc
     3001 gctgcgaagc tgtgcccgcc gcacaggctt atcaccgcgc gatagaggtc accctcgatc
     3061 ttgctgaggg tggcggccga gaccccagcg gcccgcagcg catcgtacgc ctcgatgaga
     3121 ccgacgccgg tgcgcatgga tatccgcagg gccttgatgg cgcggctgac cgctccggcc
     3181 gccgcgggca aggcgatgtc cgccgccacc caggcgcagg caatgcccca ttctttggcc
     3241 tggctgccgt cgatcttgcc atcggtgatg gtgatgccct tggcgcacgt gacgtagtcg
     3301 ctgatcagcg cgtcgacgag gtgccgcgcg atgttgagga catcgctgcg gtcgagctga
     3361 acggtctgct cgtgatacga gtcgaccggg gcggtcgcga tgtagtcgct gaccgcgtcg
     3421 ttcgggcaga acgtctggtc caggtcgggg caggtgacca ggacgaaatc gatgctgccg
     3481 gtgatgtggt gcttgaccct ggtctcgcag taccgcgatc cgctcagcgg cgggcacacg
     3541 atcggcgtct ggctgacaat ctcatcggag atgtgtggca acgcctgaac gccggcctca
     3601 ccggtcggcg agttgacgcc catcgtggct cgcgcctggt cgtccgcgtt cttctcggct
     3661 tgttccgcgg cctgctgtgc ctgctgggca tcttcgaggg cacg
//
LOCUS       DASSGQ010000295        11351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_204265,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000295 DASSGQ010000000
VERSION     DASSGQ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11351
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204265"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..432
                     /locus_tag="VFI55_13030"
     CDS             118..432
                     /locus_tag="VFI55_13030"
                     /inference="COORDINATES: protein motif:HMM:NF017148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HET7074993.1"
                     /translation="MPNVAALSGYLAVPLLAAALYAPAVAHCSPMDDYVSLNGKSVCA
                     ALDKAHSSGDVFRITLAVARDSGFSVKDAANIVGQSIAADCPWNAQAVKQTGDSAATP
                     AS"
     gene            complement(447..1541)
                     /locus_tag="VFI55_13035"
     CDS             complement(447..1541)
                     /locus_tag="VFI55_13035"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HET7074994.1"
                     /translation="MTLDLQQIRKAPKALLHDHLDGGLRPSTVIDIAGQTGYDGLPAT
                     DVDELATWFRTRSHSGSLERYLEPFSHTVAVMQTPDSLYRVAYECVEDLAADSVVYAE
                     VRFAPELHIDRGLSFDQIVDAVLEGFAAGEKACAAAGRAIKVRLLVTAMRHAAVSRRI
                     AELAIRFRDKGVVGFDIAGAEAGNPPTRHLDAFEYMRDHNARFTIHAGEAFGLPSIHE
                     AIAFCGADRLGHGVRIVDDIEVVEGGPETGGVRLGRLASILRDKRIPLELCPSSNVQT
                     GAVKSIADHPFDLLARTRFRVTVNTDNRLMSDTSMSLEMYRLVEAFGYGWSDLERFTI
                     NAMKSAFIPFDERLAIIDEVIKPRFAVLIG"
     gene            complement(1538..2848)
                     /locus_tag="VFI55_13040"
     CDS             complement(1538..2848)
                     /locus_tag="VFI55_13040"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HET7074995.1"
                     /translation="MNHSAFDAPTVIRTKRDGGRLSDAAIDWVVDAYTRGHVADAQMS
                     ALLMAIVWRGMDRGEIARWTAAMVASGERLDFTDLRTPGKPAKRLPTVDKHSTGGVGD
                     KITLPLVPVVAACGAAVPQASGRGLGHTGGTLDKLESIAGFTATLSNQRVRDQLRDVG
                     AAIFAAGELAPADAKLYALRDVTATVESLPLIASSVMSKKLAEGAGALVLDVKVGSGA
                     LMTSEAECRELAHTMVGLGAAYGVPTHALLTDMNCPLGATVGNALEVAESLEVLAGGG
                     PADVVDLTVRLAEEMLELAGIDDRDPAETLRDGTAMDRFRRLIAAQGGDLSVPLPTGS
                     CSESVTAGRGGTMGPIDAMAVGLASWRLGAGRSRPGEPVQSGAGIRIHRRPGEPVAAG
                     EPLFTLYTDTPARFGAALAELDGGWSVGDTPPLSRPLIIDRITT"
     gene            complement(2924..3343)
                     /locus_tag="VFI55_13045"
     CDS             complement(2924..3343)
                     /locus_tag="VFI55_13045"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET7074996.1"
                     /translation="MPDVEWNVLRDSAIHASRAAYAPYSRFAVGAAALVDDGRVVTGC
                     NVENVSYGLSLCAECGVVCALHSTGGGRLIALACVDAEGFSLMPCGRCRQVLLEHGGP
                     ELLIDHPDGPRRLDALLPEAFGPDDLARRRCGPEAGD"
     gene            3607..3945
                     /gene="sdhC"
                     /locus_tag="VFI55_13050"
     CDS             3607..3945
                     /gene="sdhC"
                     /locus_tag="VFI55_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HET7074997.1"
                     /translation="MWSWVLHRITGATIFFFLFVHVLDTALVRVSPQAYNEVIQTYKT
                     PIVGLMEFGLVAAVLFHALNGIRLILIDFWSQGPRHQRLMLWIVGVVSLLLIVPAGVV
                     VGIHMMEHFR"
     gene            3942..4409
                     /locus_tag="VFI55_13055"
     CDS             3942..4409
                     /locus_tag="VFI55_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase hydrophobic membrane
                     anchor subunit"
                     /protein_id="HET7074998.1"
                     /translation="MSSPDLQLGRGDTAPVKQRFYDRPASLDNPRSPRRRAGIPNFEK
                     FAWLFMRFSGIALVFLVIGHLFIMLMWQNGVYRIDFNYVAQRWHSPFWQIWDMALLWL
                     AQLHGGNGLRTIIDDYSRKNSTRFWLNSLLLLSVGFTCLLGTYVLVTFNPNIS"
     gene            4420..6174
                     /gene="sdhA"
                     /locus_tag="VFI55_13060"
     CDS             4420..6174
                     /gene="sdhA"
                     /locus_tag="VFI55_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HET7074999.1"
                     /translation="MIQQHRYDVVIVGAGGAGMRAAVEAGPRIRTAVLTKLYPTRSHT
                     GAAQGGMCAALANVEEDNWEWHTFDTVKGGDYLADQDAVEIMCKEAIEAVLDLEKMGM
                     PFNRTPEGRIDQRRFGGHTRDHGKAPVRRACYAADRTGHMILQTLYQNCVKHDVQFFN
                     EFYALDLVLTQTPTGPVATGVVAYELATGEIHVFHAKAVVLATGGSGRMYKTTSNAHT
                     LTGDGIGIVFRKGLPLEDMEFHQFHPTGLAGLGILISEAVRGEGGRLLNGEGERFMER
                     YAPTIVDLAPRDIVARSMVLEVLEGRGAGPHKDYVYIDVRHLGEDVLESKLPDITEFA
                     RTYLGVDPVHELVPVYPTCHYVMGGIPTTVTGQVLRDNTSTVPGLYAAGECACVSVHG
                     ANRLGTNSLLDINVFGRRAGIAAADYALGQEFVDMPPDPAAMVIGWVSDILSEHGNER
                     VADIRGALQQSMDNNAAVFRTEETLKQALTDIHALKERYSRITVHDKGKRFNSDLLEA
                     IELGFLLELAEVTVVGALNRKESRGGHAREDYPNRDDTNYMRHTMAYKQGSDLLSDIA
                     LDFKPVVQTRYEPKERKY"
     gene            6174..7001
                     /locus_tag="VFI55_13065"
     CDS             6174..7001
                     /locus_tag="VFI55_13065"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HET7075000.1"
                     /translation="MSVGTEGAAEVERGQEPPLPPIPDGAVMVTVKIARFNPDQPEAF
                     AETGGWQSFRVPCLPSDRMLNLLIYIKSYLDGTLTFRRSCAHGVCGSDAMRINGVNRL
                     ACKVLMRDLLPPKKEGKPDKPGKSLTITIEPIRGLPVEKDLVVNMEPFFDAYRAIKPY
                     LITSGNPPTRERIQSPTDRARYDDTTKCILCACCTTSCPVFWSEGSYYGPAAIVNAHR
                     FIFDSRDEAAGQRLDILNEVDGVWRCRTTFNCTESCPRGIEVTKAIQEVKRATMFSR"
     gene            7058..7675
                     /locus_tag="VFI55_13070"
                     /pseudo
     CDS             7058..7675
                     /locus_tag="VFI55_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            complement(7641..8564)
                     /locus_tag="VFI55_13075"
     CDS             complement(7641..8564)
                     /locus_tag="VFI55_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7075001.1"
                     /translation="MTAAQQVAEFEALRPHLMAVAYRVTGTVADAEDIVQEAWLRWDS
                     ADTRDTIADLRAWLTTVVSRLGLDRLRSAAHRREAYTGNWLPEPVVTGLDHADPLSAV
                     VASEDARFAAMVVLERLSPDQRVAFVLHDGFAVPFAEVAQVLGTSEPAARQLASRARK
                     ALAGQPASISGKPDPSHNEVVGKLMAALLEGDLAAVVALLHPDVTLTGDANGKASTAV
                     NIIHGPDKVARFMLGLARRYGPSFITANQLALINGELGAYTAGCPGVDGHWAMSPRIT
                     AMTVRDGKVCAIWDVANPDKFTGSPLRELAR"
     gene            8633..9964
                     /locus_tag="VFI55_13080"
                     /pseudo
     CDS             8633..9964
                     /locus_tag="VFI55_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878998.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
     gene            10479..>11351
                     /locus_tag="VFI55_13085"
     CDS             10479..>11351
                     /locus_tag="VFI55_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET7075002.1"
                     /translation="MSLLRSMSCLVAAIFMVAGPLVSGAPIAVAEPGPEPNAGPPGCP
                     YQVSPPAAVDSSEVPAAGDPPMPLAVPPKPVGGQALSSCGIVAAPGTPPLPGDVSADA
                     WVVADLDSGAVVAAKDPHGRHRPASIIKVLVAMASLNALPLNKSVPGTAEDAAAEGTK
                     VGVDEGGMFTVNQLLHGLLMHSGNDAAHALAMQLGGMQQALDKINVLAAKLGGRDTRV
                     ATPSGLDGPGMSTSAYDIGLFYRYAWQNPTFADIVATRTFNFPGHGDHPGYELENDNQ
                     LLYKYPGALGGKTGY"
BASE COUNT         1970 a         3965 c         3611 g         1805 t
ORIGIN      
        1 aaccggcaac cggatcacgg ggctgcctcg gcgcccgagc ttgcacagcg cggagcgctc
       61 catgttgata ccttgagttc ggcatttagc caccgccgcc ggacctggag tgaccctatg
      121 ccgaacgtcg cagcgctttc gggttacctg gccgtgccgc tactcgccgc cgctctgtac
      181 gccccggcgg tggctcactg cagcccgatg gatgactacg tcagcctgaa cggcaagtcg
      241 gtgtgcgcgg cactcgacaa ggcccacagt tcgggagacg ttttccggat cacgctcgcc
      301 gtcgcgcgtg actccggatt cagcgtcaag gacgccgcga acatcgtcgg acaatcgata
      361 gccgccgatt gcccatggaa tgcgcaggcg gtgaagcaaa ccggcgactc ggcggccacc
      421 ccggcgtcgt agccgcacaa ggccggctaa ccgatcaaca ccgcgaaccg tggcttgatc
      481 acctcgtcga tgatcgccaa ccgctcgtcg aacgggatga atgcggactt catcgcgttg
      541 atggtgaacc gctcgaggtc actccagccg tagccgaagg cctccaccaa ccggtacatc
      601 tcgaggctca tcgacgtgtc gctcatcagc cggttgtcgg tgttgacggt cacccgaaat
      661 cgcgtgcggg ccaacagatc aaacggatgg tcggcgatgc ttttgaccgc gccggtctgc
      721 acgttcgagc tgggacacag ctccagcgga attcgcttgt cccgcaagat cgacgccaac
      781 ctgcccaacc ggactccccc ggtttcgggc ccaccttcga ccacctcgat gtcatcgacg
      841 atgcggaccc catgaccgag ccggtcagcc ccgcagaacg cgatcgcctc gtggatggac
      901 ggcagcccga acgcctcacc ggcgtggata gtgaagcgcg cgttatggtc tcgcatgtac
      961 tcgaaagcgt ccaggtgccg cgtcggtggg ttgccggcct cggcgcccgc gatgtcgaaa
     1021 ccgaccactc ccttgtcccg gaagcggatc gccagctcgg cgatcctccg ggacaccgcg
     1081 gcgtgccgca tcgcggtaac cagtagccgc accttgatgg cacgacccgc tgcggcacaa
     1141 gccttttcgc cggcggcgaa accctccagg acggcatcga caatctggtc gaaggacagc
     1201 ccgcggtcga tgtgcaactc gggggcaaac cgcacctcgg cgtagaccac ggagtcggca
     1261 gccaggtcct ccacgcactc ataggcgacg cggtacagcg aatccggagt ctgcatcacc
     1321 gccacggtgt gcgaaaacgg ctccaggtag cgctccagtg agccgctgtg cgacctggtg
     1381 cgaaaccagg tcgccagctc gtcgacgtcg gtggcgggca gcccgtcgta accggtctgg
     1441 ccggcgatgt cgataacggt cgatgggcgc agtccaccgt cgaggtgatc atgcagcaga
     1501 gccttgggcg ctttcctgat ctgctgtaag tccagtgtca cgtggtgatc cgatcgatga
     1561 tgagcggcct tgaaagtggt ggtgtgtcgc cgacgctcca gccgccatcc aactcggcca
     1621 acgcggcgcc gaagcgtgcg ggggtatcgg tgtagagggt gaacagcggc tcaccggccg
     1681 cgaccggctc cccgggacgg cgatggatcc tgattccggc gcctgactgc accggttcgc
     1741 ccggacggga cctccccgcg ccgagccgcc aacttgccag gcctaccgcc atcgcgtcga
     1801 tagggcccat tgtgccgccc cggccggcgg tcacactctc ggaacacgaa ccggttggca
     1861 atggcaccga caaatccccg ccctgggccg cgatcagccg gcggaagcga tccatcgcgg
     1921 tgccgtcgcg cagcgtctcg gcggggtcgc ggtcgtctat cccggcaagc tcgagcatct
     1981 cctcggccag ccgcacggtc agatccacca cgtcggctgg cccgccgccg gccagcacct
     2041 ccagcgactc ggcaacctcg agcgcgttac cgacggtcgc acccagcgga cagttcatgt
     2101 ccgtgagcag cgcgtgagtg ggcaccccgt acgccgcgcc cagcccgacc atcgtgtgcg
     2161 cgagctcgcg acactcggcc tccgaggtca tcaacgcccc ggacccgacc ttcacgtcga
     2221 gcaccagcgc gcccgcaccc tcggccagct tcttgctcat caccgagctc gcgatcagcg
     2281 gcagcgactc gacggtcgcc gtgacatcgc gcagcgcgta cagcttggcg tcggccggcg
     2341 ccaactcgcc ggcggcgaag atggccgcgc cgacatcgcg gagctgatcg cgcacccgct
     2401 ggttggacag cgtggcggtg aacccggcaa tggattccag cttgtccagg gtgccgccgg
     2461 tatggcccag cccgcggccc gacgcctggg gcaccgcggc gccgcaggcg gcgacgacgg
     2521 gcaccagcgg cagggtgatt ttgtccccga ctcccccggt ggagtgtttg tcgacggtcg
     2581 gcagccgctt ggcgggcttg ccgggggtgc gcagatcggt gaagtccaag cgctcgcccg
     2641 acgccaccat cgccgccgtc caccgggcga tctcaccgcg gtccatgccg cgccacacga
     2701 tggccatcaa cagggccgac atctgtgcgt cggcgacgtg gccccgggtg taggcgtcga
     2761 ccacccaatc gatggcggca tcggacaacc gaccgccgtc gcgcttggtc ctgatcaccg
     2821 tcggcgcgtc gaatgcactg tggttcaagg acgttcccgg gccaaggtca atcgcccgcc
     2881 cccatctcgg accgttccgg tccgcatcgt cggcgcgcca tggtcaatcg cccgcctccg
     2941 gtccgcatcg tcggcgggct aggtcatcgg gaccaaacgc ctcgggcagc agcgcgtcaa
     3001 ggcggcgagg accatccgga tggtcgatca gcagctcggg accgccgtgc tccaggagca
     3061 cttggcggca tcgcccgcac ggcatcagcg agaatccctc cgcgtccaca cacgccagcg
     3121 cgatcagccg gcccccaccg gtcgaatgca gggcacacac cacaccacac tcggcgcaca
     3181 ggctaaggcc atatgagacg ttctccacat tgcacccggt gaccacccgg ccgtcgtcga
     3241 ccagcgcggc cgcgcccacc gcgaatcgcg agtacggcgc ataggccgcc cttgacgcgt
     3301 ggattgcact gtcccgcagc acattccact cgacgtcagg cataccgcac cctcgctcac
     3361 gtatcggccg acttgcgaca accaccctaa ccctggcacg acacagtgtc ttgatcacgc
     3421 tatttggggt accgcccgca ccattgccgg gctaaactcg cttgcccggc ttgagtgtct
     3481 ttgaacgtcc tagaaggcgg tgaggactga gtgagtacgc aggcgacaac agcggataag
     3541 ccaacagtgt ctgcatcctc gcgcaagcgg cgaccacccc ggacgctgta tcggggggat
     3601 cccggtatgt ggtcgtgggt gttgcaccgc atcaccggcg cgacgatctt cttctttctg
     3661 ttcgtgcacg tcttggacac tgccctggtc cgggtgagcc cgcaggccta caacgaggtg
     3721 atccagactt ataagacccc gatcgtgggc ctgatggaat tcggcctggt cgcggcggta
     3781 cttttccacg cgttgaacgg gatccggctg atactgatcg acttctggtc gcaggggccc
     3841 cgccaccagc ggctcatgct ctggatcgtc ggcgtcgtct ctttgcttct catcgttccg
     3901 gccggcgtgg tggtgggcat ccacatgatg gagcacttcc gatgagcagt cctgaccttc
     3961 agctgggccg cggcgacaca gcaccggtca agcaacgctt ctacgaccgc cccgccagcc
     4021 tggacaatcc ccggtccccg cgacgccgcg ccggcattcc caacttcgag aagttcgcct
     4081 ggcttttcat gcggttctcc gggatcgcgc tggtgttctt ggtgatcgga cacctgttca
     4141 tcatgctgat gtggcaaaac ggggtgtacc gcatcgactt caactatgtc gcgcagcgct
     4201 ggcattcccc gttctggcaa atctgggaca tggcactgtt gtggctggcg cagctgcacg
     4261 gcggcaacgg gttgcgcacc atcatcgacg actacagccg caagaattcc acccggttct
     4321 ggctcaacag cttgctgctg ttgtcggtgg ggtttacgtg tttgctgggc acttacgtgc
     4381 tggtgacatt caacccgaac atctcgtgaa aggcgaccgg tgatccagca acaccgatac
     4441 gacgtggtga ttgtcggcgc gggcggtgcg ggaatgcgcg ccgcggtcga ggcgggtccg
     4501 cgcatccgta ccgcggtgct caccaagctc taccccaccc gcagccacac cggagccgcc
     4561 cagggcggca tgtgcgcggc gctggccaat gtcgaagaag acaactggga atggcacacg
     4621 ttcgacaccg tcaagggcgg cgactacctc gccgaccagg acgccgtgga gatcatgtgc
     4681 aaggaagcca tcgaggcggt gctcgacctg gaaaagatgg ggatgccgtt caaccgcacc
     4741 cccgagggcc gcatcgacca gcgccggttc ggcggccaca cccgcgacca cggtaaggcc
     4801 ccggtgcggc gagcctgtta cgccgccgac cgtaccggtc acatgatcct gcagaccctc
     4861 taccaaaact gcgtcaagca cgacgtgcag ttcttcaacg agttctacgc gctggacctg
     4921 gtgctcaccc agaccccgac cgggccggtg gcgaccgggg tggtggcgta tgagctcgcg
     4981 acgggcgaaa ttcatgtatt ccacgccaag gccgtggtgc tcgcgaccgg cggctcgggc
     5041 cggatgtaca agaccacctc caacgcacac acgctgaccg gcgacggcat cggcatcgtg
     5101 ttccgcaagg gacttccgtt ggaggacatg gagtttcacc aattccaccc gaccggcctg
     5161 gccggcctgg gcatcctgat ctccgaagcg gtacgcggtg agggcggccg gctgctcaac
     5221 ggcgagggcg agcgcttcat ggagcgctac gccccgacga tcgtcgacct ggcgccgcgc
     5281 gacatcgtcg cgcgctcgat ggtgctggaa gtcctggaag gccgcggcgc cggcccgcac
     5341 aaggactacg tgtacatcga cgtccgccac ctcggcgagg acgtgctgga gtccaagctg
     5401 cccgacatca ccgagttcgc ccgcacgtat ctgggcgtgg acccggtgca cgagctggtc
     5461 ccggtctacc cgacgtgtca ttacgtgatg ggtggcatcc ccaccaccgt caccggacaa
     5521 gtgttgcggg acaacacctc caccgtcccg ggcctctatg ctgccggcga gtgcgcgtgt
     5581 gtgtcggtgc acggcgcgaa ccggctgggc accaattcgc tgctggacat caacgtcttc
     5641 gggcgccggg ccggcatcgc ggccgccgac tacgcgctgg ggcaggaatt cgtcgacatg
     5701 ccaccggacc cggctgcgat ggtcatcggc tgggtgagcg acatcctctc cgagcacggc
     5761 aacgaacgcg tcgccgatat ccgtggcgcc ctgcagcagt cgatggacaa caacgccgcg
     5821 gtgttccgca ccgaggagac gctgaagcag gcgctcaccg acatccacgc gctcaaggag
     5881 cgctactccc gaatcaccgt gcacgacaaa ggcaaacgct tcaacagcga tctgctggaa
     5941 gccatcgagc tgggcttcct gctggagctg gccgaggtca ccgtcgtcgg cgcattgaat
     6001 cgcaaggagt cgcgcggcgg ccacgcccgc gaggactacc cgaaccgcga cgacaccaac
     6061 tacatgcgcc acaccatggc ctacaagcag ggcagcgact tgctgagtga catcgcgctg
     6121 gacttcaagc ccgtcgtgca gacccgctac gaacccaagg aacggaaata ctgatgagcg
     6181 tcggaaccga aggggccgca gaggtcgagc gcgggcagga acctccctta ccgccgatcc
     6241 ccgacggcgc ggtgatggtg acggtgaaga tcgcccggtt caaccccgac cagcccgagg
     6301 ccttcgcgga aaccggcggc tggcaaagct ttcgggtacc gtgcctgccc agcgaccgga
     6361 tgctcaacct gctgatctac atcaagagct acctggacgg gacgctgacc ttccggcgct
     6421 cctgtgccca tggggtgtgc ggctccgacg cgatgcgcat caacggcgtc aaccggctgg
     6481 cctgcaaggt gctgatgcgc gacctgctgc ccccgaagaa ggagggcaag ccggataagc
     6541 ccggaaaatc gctgacgatc accatcgagc cgatccgcgg cctgccggtg gagaaggact
     6601 tggtcgtcaa catggaaccg ttcttcgacg cctaccgggc gatcaagccc tacctgatca
     6661 ccagcggcaa tcccccgacg cgggaacgca ttcagagccc gaccgaccgt gcccgctacg
     6721 acgacaccac caagtgcatc ctgtgcgcgt gctgcaccac cagctgcccg gtgttctgga
     6781 gcgagggcag ttactacggc ccggccgcga tcgtcaacgc ccaccggttc atcttcgaca
     6841 gccgcgatga ggccgcgggt cagcgtctcg acatcctcaa cgaggtcgac ggcgtctggc
     6901 ggtgccgaac cacgttcaac tgcaccgaat cctgcccgcg cggcatcgaa gtcaccaagg
     6961 cgatccaaga ggtcaagcgc gcgacgatgt tctcgcgtta atcccccagc gtggcgttag
     7021 caggccccgg gcgccgtcac acattgccgg cctgtctcgt ctgacgggta tgaaacagaa
     7081 aacccgcatc gagcccctgc cgccacggcg cgccggacgg ctgacccgag ccatgtattg
     7141 ggtggccaag cggcgctacg ggcaagtccc cgaaccgttc gccgtcgccg cacaccaccg
     7201 cggactgatg gtcgccggcg ccgtgcacga gaccatgatc gaccgggcgt cgaaatcgct
     7261 gccggtcagc gtgcgcgaac tggcggtgtt ctggaccgcg cgcacgatcg gctgctcgtg
     7321 gtgcgtcgac ttcgcgtcca tgctgcagcg cctggacggg ctggacatca cgcgactcga
     7381 agacatcgac aattacgcca cctcaacggc tttcaccgac aacgagcgcg ccgcgatcgc
     7441 gtacgccgac gcgatgacca ccgatcccca caccgtcacc gacgagcagg tcgccgacct
     7501 gaaagcccgt ttcggcgacg ccggggtgat cgagctgacc tatcaaatcg gagtggagaa
     7561 catgcgggcg cggatgaacg cggcgctggg aatcaccgag cagggcttca attccggcga
     7621 tgcgtgccgc atcccgtggg ctagcgcgcc agctcgcgca acggagagcc ggtgaacttg
     7681 tcggggttgg cgacatccca tatggcgcag acctttccgt ctcgcaccgt catcgcggtt
     7741 atccgcggcg acatcgccca atgcccgtca acaccggggc aacccgcggt gtaggcgccc
     7801 agttcgccgt tgatcagagc cagctgattt gccgtgatga acgacgggcc gtagcggcgg
     7861 gccaggccga gcatgaaccg ggcgaccttg tccgggccgt ggatgatgtt gaccgccgtc
     7921 gacgccttgc cattggcatc cccggtcagc gtcacgtcgg gatgcaacaa cgccaccacg
     7981 gcggccaggt cgccctcgag caacgcggcc atcagcttgc cgacaacctc gttgtgcgag
     8041 ggatccggct tcccggatat cgaggccggc tgcccggcca gcgccttgcg ggctcgcgac
     8101 gccagctgcc gggcggcggg ctcgctggtt cccagcacct gcgccacctc ggcgaacggc
     8161 accgcgaacc cgtcgtgcag cacgaacgcg acccgctgat cgggggacag ccgctccagc
     8221 accaccatcg ccgcgaaccg ggcatcctcg ctggccacca cggcggacag cggatccgcg
     8281 tgatcaagac cggtgaccac cggttcgggc agccagttgc cggtgtaggc ctcgcgacgg
     8341 tgagcggccg accgcaaccg gtccaggccc agccggctca ccacggtcgt cagccaggcc
     8401 cgtaagtcgg cgatcgtgtc gcgggtgtcg gccgagtccc agcgcagcca ggcttcctgg
     8461 acgatgtcct cggcgtcggc cacggtgccg gtcacgcggt aggcgaccgc catgagatgc
     8521 ggtcgcaggg cttcaaattc ggcgacctgc tgcgctgcgg tcatacgccg agcgtaacgc
     8581 caccgatgct ttcgacgccg aaagtcgcag tggcgttacg ctcgcggaca caatgactga
     8641 acatctttgg ctgcacttcg cccgccacgg cccgcgcatc acgccgccga tcatcacccg
     8701 cggcgagggc gtcaccatct tcgacgaccg cggcaagagc tacctggacg cgctgtccgg
     8761 gctgttcacc gtgcaggtcg gtcacgggcg cagcgaactc gccgaggccg ccgcccggca
     8821 ggcgcagacg ctggcgttct tcccgctgtg gggctacgcc accccgaccg cgatcgagct
     8881 ggccgggcgc ctcgcacacc acgccccggg cgacctgaac cgggtcttct tcaccaccgg
     8941 cggcaccgag gccgtcgaaa ccgcgtggaa ggtggccaag cagttcttca agctgaccgg
     9001 caaacccggt aagcacaaag tgatttcgcg gtcgatcgcc taccacggca ccacccacgg
     9061 tgcgctcgcg atcaccgggc tgccgttgta caaggcgccg ttcgagccgg tgacgcccgg
     9121 cgggttccgg gtgcccaaca cgaacttcta ccgggcgccc gaaccgtttc acaccgacgt
     9181 caaggcattc gggcggtggg ccgccgaccg catcgccgag gcgatcgagt tcgaggggcc
     9241 ccagaccgtt gccgcggtgt tcctggagcc ggtgcaaaac gccggtggct ccatcccgcc
     9301 gccgccgggc tatttcgagc gggtccggga gatctgcgac gaatacgacg tgctgctggt
     9361 ctccgacgag gtgatctgcg cgttcggccg gatcgggtcg atgttcgcct gtgaggactt
     9421 cggctacgtg cccgacatga tcacctgcgc caagggaatg acgtcgggct actcaccgat
     9481 cggcgcgatg atcgccagtg agcggttatt cgagccgttc aacgacggca acaccatgtt
     9541 cccgcacggc tacacgttcg gcggccaccc cgtgtcggcg gccgtcgggc tggccaacct
     9601 cgacatcttc gagcgcgagg gtctcaacga ccgtgtcaaa cgactcgcgc ccggcttccg
     9661 cgccaccctg gagaagctct acgacctgcc catcgtcggc gacgtacgcg gcgagggcta
     9721 cttctacagc gtcgaactcg tcaaggacca ggcgacgaag caaaccttca ccgacgacga
     9781 acgccggccg ctactggcgc gggtgtcttc ggcgatgttc gaggccgggt tgtactgccg
     9841 caccgacgag cgcggcgatt ccgtcatcca actggcgcca ccgctgatca gcggtcaagc
     9901 cgaattcgac accattgagg cgatcctgcg cagcgtgctc agccaggagt cccagcggct
     9961 ttagccgcgg cccgcttgac ctcgtcggca gccatcagca cttcccgcga atgcggcttg
    10021 ctcggttgct tcgccagccg cgtttgtgcg ttgcaaagcc aggcgagcgc cgaacgcagg
    10081 tccggatcgg acgtgctgtc gcgatgttgc ttcagctccg ccagcacctg gtcgagcccg
    10141 gagcgccgaa cctgggtccc cttctcgacc tccttgagca gctggttcag cgatcgccga
    10201 gcggggccgg gcgaatcgcc cgcgccagcg cggttgcgcc gccactttga ccaaaatcga
    10261 acgtttggaa tggcctaccc tttggattct ttggcaccgt gtggacttgg ggtgcggggt
    10321 tcggccggcg tcgcgccgac cgcatgtcag cgtagttggt cgactcctcc gccgccggtc
    10381 gggaaccgga ccgacgcgcg taatttgtaa catctgtccc accagtcaca gcgcggctcc
    10441 cgggaattcg gcgctcgatc gcctaatctg cacaggcaat gagcctctta cgttcgatgt
    10501 catgcctggt cgcagcgatt ttcatggtgg ccggacccct cgtgtcgggg gcgccgattg
    10561 cggtcgccga accgggtccc gagcccaatg ccggtccacc aggctgccct taccaggtgt
    10621 ctcccccagc cgcggtggac tcgtcggagg tccccgccgc cggtgacccg ccgatgccgc
    10681 tggcggtgcc ccccaagccg gtcggcggcc aggcgctgag cagctgcggc atcgtcgccg
    10741 cgcccggcac accgccgctg ccgggcgacg tgtccgccga cgcatgggtg gtggcggacc
    10801 tggacagcgg cgcagtcgtc gcggccaagg acccgcacgg ccggcaccgc ccggccagca
    10861 tcatcaaggt gctggtcgcg atggcatcgc tcaatgcgct gcccctcaac aagtccgtcc
    10921 cgggaaccgc cgaggacgcg gccgccgaag gcaccaaggt cggcgtcgac gagggcggca
    10981 tgttcaccgt caaccagctg ctgcatgggc tgctgatgca ctccggcaac gacgccgccc
    11041 acgcgctggc catgcagctc ggcggcatgc agcaagcgct ggacaagatc aacgtgctgg
    11101 ccgccaagct cgggggccgc gacacgcggg tggcgacgcc gtccgggctg gacggccccg
    11161 gcatgagcac ctcggcctac gacatcggcc tgttctaccg gtacgcctgg cagaacccca
    11221 cgttcgccga catcgtcgcg acgcggacct tcaacttccc cggccacggc gaccaccccg
    11281 gttacgagct ggagaacgac aaccagctgc tctacaagta cccgggtgcg ctgggcggca
    11341 agaccggcta c
//
LOCUS       DASSGQ010000296        16879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_811644,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000296 DASSGQ010000000
VERSION     DASSGQ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 16879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16879
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811644"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /locus_tag="VFI55_13090"
     CDS             <1..1441
                     /locus_tag="VFI55_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019731509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HET7075003.1"
                     /translation="LIYPEMRELVRAHQRRGHTVVLSSSALTIQVEPVARFLGIPNTL
                     TNKFETNQDGLLTGRVQKPILWGPGKAAAVQRFAVQHHIDLKDSYFYADGDEDVALMY
                     LVGNPRPTNPEGKMAAVAKRRGWPILRFNSRGPVGLRRQVRTLAGLGSMFPIAAGAVG
                     IGVLTGSRRRGVNFFTSTFSQLLLATSGVHLNVIGRENLNAQRPAVFIFNHRNQVDPV
                     IAGALVRDNWIGVGKKELEKDPIMGTLGKLLDSVFIDRDDPAAAVESLHTVEERARNG
                     LSIVIAPEGTRVDTTEVGPFKKGPFRIAMAAKIPIVPIVIRNAEIVAARNSTVINPGT
                     VDVAVFPPISVQDWTVETLPEHIAEVRQLYLDTLADWPATELAELPQGGLDAEKKAAA
                     KAKAGPPATPKAAAKVAAKKTATKAASKSGAKTSSKSAAKKVAGKPKPAAPTPKVSEN
                     AVTDGQAQQPSVGEAVSAESPSSPPRGRP"
     gene            1438..3804
                     /locus_tag="VFI55_13095"
     CDS             1438..3804
                     /locus_tag="VFI55_13095"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase"
                     /protein_id="HET7075004.1"
                     /translation="MTRASVDTSAVLTAQESLVLASMASPVEKELIMAWVGRQRAGNP
                     EAKFEVLELPPLDAPPLALTSLAEELVSGTEVNDDRSVVPVRVFWLPPADRGRVAKLA
                     GLLPGRDPYQPNQRQQRHILRHDAQRARVVAGESAKVSELRQQWRDTTVGGNERDFAQ
                     FVTRRAVLALERAEYRILGPQYKSPRLVKPEILASTRFRAGLNEIPGATVEEAGKMLD
                     ELATGWSRVSVDLVGVLGRALSRGFDPEIDYDEYQVAAMRAGLDAHPAVLLFSHRSYI
                     DGAVLPVAMQENRLPPVHVFAGINLSFGVMGPLLRRAGTIFIRRNIGDDPLYKYVLRE
                     YVGYIVEKRFNLSWSIEGTRSRTGKMLPPKLGLMAYVADAYLDGRSEDILLQPVSISF
                     DQLHETAEYAAYARGGEKTPEGIGWLYSFIRAQGERNYGKIYVRFPEAVSMRQYLGPP
                     HGPLAEDEDAKRLALQKMSFEVAWRILRATPVTATGLVCALLLTTRGAALTLDQLQHT
                     LQDSLDYLERKRTPMSTSALRLRSRDGVRAAVDTLSNGHPITRVDGGREPVWLIAPQH
                     EHAAAFYRNSVIHAFLETSIVELALAHARHVDGDRMAAFWAQAMRLRDLLKFEFYFAD
                     SAAFRENIAAEMAWHDDWEAHVAAGGDEIDAMLFAKRPLMSDAMLRVFFEAYEIVADV
                     LRDAPANIGQKELTESALGIGRQYVAQTRVRSSESVSTLLFATAFQVVVDQDLVAPGP
                     DLAERRTAFRRELRNILRDFGYVERIARDQFIARESKARQERLDSHTR"
     gene            3833..5884
                     /locus_tag="VFI55_13100"
     CDS             3833..5884
                     /locus_tag="VFI55_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET7075005.1"
                     /translation="MTVAPLAGKASDKAPARTPVWPLLIGVAVLAGCTAAGIGTLSLA
                     AALTATGLPDPGPATTLGLPFVRAAGEIAAVLAVGSFLCAAFLVPPQRNGVLDAGGYR
                     ALRLGTVASGVWAVCAALLVPLTISDVSGHPVTDLNPAAVWSLAGLINTASAWRWTAI
                     LAATVTLASLSVLRWSWTPLLLGGSLVTLIPLGLTGHSSAGGSHDLATNSLLIHLVAA
                     SLWAGGLLALLAHALRNGEHTGLAARRFSTIALWCWVAMALSGMVNALVRILPSDLLT
                     TAYGRLVVAKFVALCLLGVLGWRQRRSGVAALQADPSPPGARSGLIRLALIEAVVFGL
                     TFGIAVGLGRTAPPPPAVRLPSIPEAEIGYDFDGPPTVARILFDWRFDLIFGSLAIVF
                     AGLYVAAVLRLHRRGDRWPAGRTVAWLVGCSALLFVSSSGVGRYMPAMFSMHMIAHMG
                     LSMLVPIFLVLGAPVSLALRALPAAGRNDPPGMREWLLAALHSRLSRFLTNPVVATAL
                     FVAGFYGLYLSNLFDTTVSSHAGHLAMNLHFLLSGYLFYWVVIGVDPTPRPIPPLAKV
                     AVVFASLPLHAFFGIVLMGTRKVLGADYYRSLGLSWHTDLLGDQRLGGGIAWAAGELP
                     LVIVMIALLVQWARSDKRTARRLDRAAELDDDADLTAYNAMLAELARRETPGRSHQPA
                     D"
     gene            6055..6498
                     /locus_tag="VFI55_13105"
                     /pseudo
     CDS             6055..6498
                     /locus_tag="VFI55_13105"
                     /inference="COORDINATES: protein motif:HMM:NF004512.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            6619..8295
                     /gene="ettA"
                     /locus_tag="VFI55_13110"
     CDS             6619..8295
                     /gene="ettA"
                     /locus_tag="VFI55_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HET7075006.1"
                     /translation="MAEFIYTMKKVRKAHGDKVILDDVTLSFFPGAKIGVVGPNGAGK
                     SSVLRIMAGLDKPNNGDAFLATNATVGILQQEPPLNEEKTVRGNVEEGLGEIKVKLDR
                     FNEVAELMATDYSDELMEEMGRLQEDLDHADAWDLDSQLEQAMDALRCPPPEEPVTNL
                     SGGERRRVALCKLLLSKPDLLLLDEPTNHLDAESVQWLEQHLAAYPGAILAVTHDRYF
                     LDNVAEWILELDRGRAYPYEGNYSTYLEKKAERLSVQGRKDAKLHKRLTEELAWVRSG
                     AKARQAKSKARLQRYEEMAAEAEKTRKLDFEEIQIPVGPRLGNVVVDVQHLDKGYDGR
                     TLIKDLSFTLPRNGIVGVIGPNGVGKTTLFKTIVGLEQPDSGTVKVGETVKLSYVDQA
                     RAGIDPKKNVWQVVSDGLDYIEVGRTEVPSRAYVSAFGFKGPDQQKPAGVLSGGERNR
                     LNLALTLKQGGNLILLDEPTNDLDVETLSSLENALVNFPGCAVVISHDRWFLDRTCTH
                     ILAWEGDEDNEAKWFWFEGNFGAYEENKVERLGVDAARPHRLTHRKLTRD"
     gene            8398..13253
                     /locus_tag="VFI55_13115"
                     /pseudo
     CDS             8398..13253
                     /locus_tag="VFI55_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774116.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
     gene            13250..13666
                     /locus_tag="VFI55_13120"
     CDS             13250..13666
                     /locus_tag="VFI55_13120"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET7075007.1"
                     /translation="MSTGFVAPVPVRWSDIDMYQHINHATMVTILEEARVPFLKEPFA
                     ADITTIGLLIADVRVTYKDQLRLADSPLQVTMWTKRLRAVDFTLGYEVRSANSRPESK
                     PAVVAESQLAAVHIEEQRLVRLSPHHREYLQRWVRE"
     gene            13672..14334
                     /locus_tag="VFI55_13125"
     CDS             13672..14334
                     /locus_tag="VFI55_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075008.1"
                     /translation="MAERGLWLGPDGRAAHRADLATFVDHAMRLDDAAVVRLRARSAG
                     LISAWVATGFDVLASRVVVGKVRPDDLSVGADALARGLSAMDASGYVDPGFSMDAAWR
                     GALPPESGFTHLDDVPARVMLDLAQRGARLAREHGSSHGPPVSLLDQEVIRVSSADIS
                     VGLPMRCVFALTAMGFLPQSPDAIAADEMVRVRILPSWLRVDARFGSVYRRRSHAALV
                     LR"
     gene            complement(14341..15936)
                     /locus_tag="VFI55_13130"
     CDS             complement(14341..15936)
                     /locus_tag="VFI55_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HET7075009.1"
                     /translation="MEPGTPRARAPRPGPAWWSNAVFYQVYPRSFADSNGDGVGDLDG
                     LTARLGYLEKLGVDSIWLNPVTVSPMADHGYDVADPRDIDPLFGGMAAIERLIAAAQE
                     RGIKITMDVVPNHTSSAHPWFQAALAAGPGGDARDRYIFRDGRGPGGESPPNNWTSVF
                     GGSAWTRVAEPDGNPGQWYLHLFDAEQPDLNWDNTEVFDDFEKTLRFWLERGVDGFRI
                     DVAHGMAKPPGLPDAKDDVKVLSHSDDDPRFNHPSVHEIHRGIRKIVDDYRGAVTIGE
                     VWVMDNLLWAEYLRPDELHLGFNFRLTKIGFDATEIHDAIQNSLTATAIENAIPTWTL
                     SNHDVGREVTRYGGGEIGLRRARALAMLMLALPGAVFIYNGEELGLPDVLDLPEDVLQ
                     DPTWERSGHTERGRDKCRVPIPWSGDTPPFGFSTTADTWLPMPADWAALTVEKQSADP
                     ASTLSFFQRAIELRRQRPEFDGDLQWQTAPPDALMFGRGGGLVCALNAGSHPMALPAG
                     EVILASGPLDGGKLPPDAAAWLI"
     gene            complement(15985..16374)
                     /locus_tag="VFI55_13135"
     CDS             complement(15985..16374)
                     /locus_tag="VFI55_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003913747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HET7075010.1"
                     /translation="MDQESFYDAVGGAETFAAIVSHFYAQVPEDEILSQLYPADDLAA
                     AEERLRMFLEQYWGGPRTYSDQRGHPRLRMRHAPFRITPIERDAWLRCMHTAVASIDS
                     NTLDDEHRRELLDYLEMAAHSLVNSAF"
     gene            16528..>16879
                     /locus_tag="VFI55_13140"
     CDS             16528..>16879
                     /locus_tag="VFI55_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET7075011.1"
                     /translation="MAHGSRRRSHRSPGAAAGITGPATASSLHSVEIYPRVENYARVE
                     THPPGRHEAASIWSRRRVLLLNSTYEPLTALPMRRAIVMVICGKADVVHHDPAGPVIH
                     SATSSIVVPSVIQLR"
BASE COUNT         2846 a         5600 c         5637 g         2796 t
ORIGIN      
        1 cctgatctac ccggagatgc gcgaactcgt ccgtgcccac cagcgccgcg gccacaccgt
       61 ggtgctgagc tcgtcggcgc tgaccatcca agtcgagccg gtggcgcgct tcctcgggat
      121 cccgaacacg ctcaccaaca agttcgagac caaccaggac gggttgctca ccggacgcgt
      181 gcagaaaccc atcttgtggg gcccgggcaa agccgctgcg gtacaacggt ttgccgtcca
      241 gcaccacatc gatctcaagg acagctactt ctacgccgac ggcgacgagg acgtcgccct
      301 gatgtacttg gtcggcaatc cacgcccgac caaccccgaa ggaaagatgg ccgccgtggc
      361 caagcgccgc ggctggccga ttctgaggtt caacagtcgc ggtccggtgg gcctgcggcg
      421 gcaggtgcgc acgcttgccg gtttgggttc tatgttcccg atcgccgccg gcgcggtggg
      481 gatcggcgtg ctgaccggta gccggcggcg gggcgtcaac ttcttcacct ccaccttttc
      541 ccagttgttg ctggcgacca gcggggtgca tctcaatgtc atcggcagag agaatctgaa
      601 cgcgcagcgg ccggcggtgt tcatcttcaa tcaccgcaac caggtagacc cggtcatcgc
      661 cggtgcgctg gtgcgcgaca actggatcgg cgtgggcaag aaggaactgg agaaggatcc
      721 gatcatgggc acgctcggca agctgctgga cagcgtgttc atcgaccggg acgatccggc
      781 cgcggcggtg gagtcgctgc acaccgtcga agagcgggcc aggaacgggc tgtcgatcgt
      841 gatcgccccg gaaggcaccc gggtcgacac caccgaggtt gggccgttca agaaggggcc
      901 gttccgcatc gcgatggccg cgaagatccc gatcgtcccg atcgtgatcc gcaacgcgga
      961 gatcgttgcc gctcgcaact ccaccgtgat caaccccggc accgtcgatg tcgcggtgtt
     1021 cccgccaatc tcggtgcagg actggacggt tgagacgctg ccggaacaca tcgccgaggt
     1081 gcgtcagctg tatctggaca cgctggccga ctggcccgct acagagctgg ccgaactgcc
     1141 ccaaggcggg ctggacgccg agaaaaaggc ggcggcaaag gccaaggctg ggccccccgc
     1201 gacccccaaa gccgccgcga aggtcgccgc caaaaagact gcgaccaagg cggcttccaa
     1261 gtcgggcgcc aagacctcgt ccaagtctgc ggcgaagaag gttgctggca agccaaagcc
     1321 ggccgcgccg actccgaagg tgtccgagaa cgctgtcaca gacggtcagg cacaacagcc
     1381 gagcgtgggg gaggccgtca gcgctgaatc gccgagttcc ccgccccgag ggcggccgtg
     1441 accagagcgt ccgtggatac cagcgcagtc ctcaccgcgc aagagtcgct ggtattggcg
     1501 tccatggcct cgccggtcga gaaagagctg atcatggcct gggtgggccg gcagcgtgcc
     1561 ggcaaccccg aggcgaagtt cgaggtgttg gagctgccgc cacttgacgc gccgccgctg
     1621 gcgttgacgt cgttggcgga ggagctcgtg tccggcaccg aagtcaacga cgaccgttcg
     1681 gtcgtgcccg tgcgggtgtt ctggctaccg ccggcggatc gcgggcgagt cgccaagctg
     1741 gccgggctgc tgcccggccg ggatccctac cagcccaacc aacgtcagca gcgacacatc
     1801 ctgcgacacg acgcgcaacg cgcgcgtgtg gtggccggcg agtcggccaa ggtgtccgaa
     1861 cttcgccagc agtggcgcga caccacggtt ggagggaacg agcgcgactt cgcgcagttc
     1921 gtcactcgcc gcgccgtttt ggcgctggaa cgggccgaat accggatcct gggaccgcaa
     1981 tacaagtctc cgcggctggt gaaaccggag atcttggcgt ccacccgatt ccgcgcgggc
     2041 ctcaacgaaa tcccgggcgc gacggtagaa gaagccggga agatgctcga cgagctcgcc
     2101 accgggtgga gccgcgtgtc cgtcgacctg gtcggcgtcc tcggcagggc tctcagccgc
     2161 ggattcgatc cggagatcga ctacgacgag taccaggtcg cggcgatgcg tgccgggctg
     2221 gacgctcatc cggcggtcct gctgttctcg caccggtcct acatcgacgg tgcggtgctg
     2281 ccggtggcga tgcaggagaa ccggctgcct ccggtgcacg tgttcgccgg catcaacctg
     2341 tcgttcggtg tgatggggcc gctgctgcgc cgtgccggca cgatcttcat ccgccgcaac
     2401 atcggcgacg acccgctgta taaatacgtc ctgcgcgagt acgtcggcta catcgtcgaa
     2461 aagcggttca acctgagctg gtccatcgaa ggcacccgct cgcgtaccgg caagatgctg
     2521 ccgcccaagc tgggactgat ggcttatgtg gccgacgcct atctggacgg gcgcagcgaa
     2581 gacatcctgc tgcagccggt gtcgatcagc ttcgaccagt tgcacgagac cgccgagtac
     2641 gccgcctacg cccgcggcgg cgaaaagaca cccgagggca tcggctggct gtacagcttc
     2701 atcagggcgc agggcgaacg caactacggc aagatctacg tccgcttccc cgaggcggtc
     2761 tcgatgcgcc agtacctcgg ccctccgcac ggtccgctgg ccgaagatga ggacgccaaa
     2821 cggcttgcgc tgcaaaagat gtcgttcgaa gtcgcgtggc gaattcttcg ggcaacgccg
     2881 gtgacggcca cgggtttggt gtgtgccttg ctgctgacca cccgcggtgc ggcgttgacg
     2941 ctcgatcagc tgcagcacac tttgcaggac tcgctcgact acctcgaacg caagcggact
     3001 ccgatgtcga cgagcgcgtt gcggctgcgt tcgcgcgacg gcgtgcgcgc ggccgtcgac
     3061 acgctgtcca acgggcaccc gatcacccgg gtcgacggcg gccgggagcc ggtgtggctc
     3121 atcgcgcccc agcatgaaca cgccgcggcg ttctaccgca actcggtgat tcacgccttt
     3181 ctggagacct cgatcgttga gctcgcgttg gcgcacgccc gtcacgttga cggggaccgg
     3241 atggcggcct tctgggccca ggcgatgcgg ttgcgcgatc tgctgaaatt cgagttctat
     3301 ttcgccgact ccgcggcgtt ccgggaaaac atcgccgcag agatggcgtg gcacgacgat
     3361 tgggaagccc acgtcgccgc agggggcgac gagatcgacg cgatgctgtt cgccaagcgt
     3421 ccgttgatgt cggacgcgat gttgcgggtg ttcttcgagg cgtacgagat tgtcgccgac
     3481 gtgctgcgcg acgcgccggc gaatatcggc cagaaggaac tcacggagtc ggcgctcgga
     3541 atcggccggc aatacgtggc gcagacccgg gtgcgcagca gcgaatcggt atcgactctg
     3601 ctgttcgcca ccgcattcca ggtcgtcgtc gaccaggatc tggtcgcgcc gggcccggac
     3661 ctcgccgaaa gacggaccgc attccggcgg gaattgcgca acattctccg agatttcggt
     3721 tacgtcgagc ggatcgcgcg cgaccagttc atcgcccgcg agtccaaggc gcgccaggag
     3781 cgtctggaca gccacacgcg atagccggct cgtacgccaa taccctgggt gcatgacggt
     3841 tgccccgctc gccggcaagg cctcggacaa ggcccccgcc cgcacgccgg tgtggccgct
     3901 gctgatcggc gtcgcggtgc tggcgggctg caccgcggcc ggaatcggaa cgctgtcgct
     3961 ggcggccgcg ctgaccgcca cgggtctacc ggatccgggc ccggcaacca cgctggggct
     4021 gccgttcgtc cgggccgccg gggagatcgc cgcggtgctg gccgtcgggt cgttcctgtg
     4081 cgcggcgttc ctggtgccgc cgcaacgcaa cggtgtgctc gatgcgggcg ggtaccgcgc
     4141 gcttcgcctc ggcacggtgg cgtcgggggt atgggcggtg tgtgccgcgt tgctggtccc
     4201 gctgaccatc tcggacgtgt ccggtcaccc cgtgaccgac ctcaatccgg ccgcggtctg
     4261 gtcgctggcc gggctgatca acaccgcgtc cgcgtggcgc tggaccgcga tcctggccgc
     4321 gacggtaacg ctggcgagtc tgtcggtgct gcgctggtcg tggacgccgc tgctgctggg
     4381 cgggtcgttg gtgacgctga ttccgctcgg gctgacgggt cactcgtcgg ccggcggctc
     4441 ccacgacctg gccaccaaca gcctgctcat ccacctcgtc gcggccagcc tgtgggccgg
     4501 cggtctgctc gccctgctcg cgcacgcgct gcgcaacggc gaacacaccg gcctggcggc
     4561 gcgacggttc tcgacgatcg cgctgtggtg ttgggtggcg atggcgctca gcgggatggt
     4621 gaacgccctg gtgcggatcc tgccgtcgga cctgctgacc actgcgtacg ggcgactggt
     4681 ggtggccaag ttcgtcgcgc tttgcctgct gggcgtcctg ggctggcgac agcggcgctc
     4741 cggagtggct gcgctgcagg cggatccgag tcctcccggt gcgcgcagtg ggctgatcag
     4801 gctggcgctg atcgaggccg tcgttttcgg cctcaccttc ggcattgccg tcgggctggg
     4861 ccgcacggcg ccgccaccgc cggccgtccg gctgccctcg attcccgaag ccgagatcgg
     4921 ctacgacttc gacggaccgc ccacggtggc gcgcatcctg ttcgactggc gcttcgacct
     4981 gatcttcggc agcctcgcga tcgtcttcgc cgggctgtat gtggcagcgg tgctgcggct
     5041 gcatcgccgc ggcgaccgct ggccggcggg ccggaccgtg gcctggctgg tcggctgctc
     5101 ggcgttgctc ttcgtgagct cgtcgggggt cggtcgctac atgccggcga tgttcagcat
     5161 gcacatgatc gctcacatgg ggctttccat gttggtgccg atcttcctgg tgctgggcgc
     5221 tccggtgagc ctggcgttac gggcgctgcc cgccgccggt cgcaatgatc cgcccggtat
     5281 gcgggagtgg ctgctggccg cgctgcatag caggttgtcg cggttcctca ccaacccggt
     5341 ggtcgctacc gcactgttcg tcgcggggtt ctacgggctg tacctgtcga accttttcga
     5401 caccacggtc agcagtcacg cagggcattt ggcgatgaac ctgcactttc tgctcagcgg
     5461 gtatctgttc tactgggtcg tgatcggcgt ggatcccacg ccgcgtccga taccgccgct
     5521 ggccaaggtg gccgtggtgt tcgcgtcgtt gccgctgcac gcgtttttcg ggatcgtgct
     5581 gatgggcacc cgcaaggtac tcggcgccga ctattaccgc tcgctgggtt tgagttggca
     5641 caccgacctg ctgggtgatc agcggttggg cggtggaatc gcctgggcgg caggggaact
     5701 gccgctggtg atcgtcatga tcgcgctgct cgtgcagtgg gcccgcagcg ataagcgcac
     5761 cgcccgccgc ttggaccggg ctgccgagct ggacgatgac gccgacctga ccgcctacaa
     5821 cgcgatgctg gccgaactgg cgcggcgtga aacgccgggc cgttcgcacc aaccagcgga
     5881 ctaatccaca gccacgtttc cttccacagg ggcagcgggc agcggccgtg tttgcgccgc
     5941 tttggcgcgg accccgtcgg tggcgccgag ctaaatggcg gcaggaaagg gcgctgacac
     6001 gttcaccgag ccggtcggca agcccgagaa atacaaccga gaagggacca gccaatgttc
     6061 gaaaccccgc ttaccgtggt cggacacatc gtcaacgacc cgcagcgccg caaggtcggc
     6121 gatcaggagg tcatcaagtt ccgggtggcc agcaattcgc gccgccgcac cgccgacggc
     6181 agctgggagc cgggcaattc gctgttcatc accgtcaact gctgggggaa gctggtcacc
     6241 ggcgtgggcg ccgcgctcgg caagggcgca cccgtgatcg cggtgggcca cgtgtacacc
     6301 agcgagtacg aggaccgcga cggcaatcgc cgctcatcac tggagatgcg ggcgaccacg
     6361 gtcggtcccg acctgtcgcg tgtcttcgtg cgcatcgaga agcccggata caccggcccc
     6421 gacgcggatc ccgcctcgac gattgccgtc accgcaacta ctgatgaggt tgccgacgac
     6481 ccggtcgccg ctggtgacgc cgccgctgat cccggcccgc tgccactgtc ggcttagctg
     6541 cgagtccatt gcctccgggc ggtgcctagg atggtccgcg agatcatcct gaaaaaacca
     6601 gaaaggcaaa accgcggcat ggctgagttc atctacacga tgaagaaggt ccgcaaggcg
     6661 cacggcgaca aggtgatttt ggacgacgtc acgttgagct tctttccggg cgccaagatc
     6721 ggcgtcgtcg gccctaacgg cgccgggaag tcgagcgtct tgcggatcat ggccggtctg
     6781 gacaagccga acaacggtga cgccttcctg gccaccaacg ccaccgtcgg catcctgcaa
     6841 caggagccgc cgttgaacga ggagaaaacc gttcgcggca acgtggaaga gggcctgggc
     6901 gaaatcaagg tcaagctgga ccgtttcaac gaggtggccg agttgatggc caccgactac
     6961 tccgatgagc tgatggagga aatgggccgg ctccaagagg acctcgacca cgccgacgcc
     7021 tgggacctcg actcgcagct ggagcaggcg atggacgcgc tgcgctgccc gccgcccgag
     7081 gaaccggtca cgaatctgtc gggcggtgag cgccgccgcg tggcgctgtg caagctgctg
     7141 ctgtccaaac ccgacctgtt gctgctcgac gagccgacca accacctgga cgccgaaagc
     7201 gtgcagtggc tcgaacagca cctggccgcc tacccgggcg cgattttggc ggtgacccac
     7261 gaccgctact tcctggacaa cgtcgccgag tggatcctcg agctcgaccg tggccgcgcg
     7321 tatccctacg aaggcaacta ctcgacctac ttggagaaaa aagcggaacg cctgtcggtg
     7381 cagggccgca aggacgccaa gctgcacaag cggctgaccg aggagctggc ctgggtccgg
     7441 tccggggcca aggcgcgcca agcgaaaagc aaggcgcgcc tccagcgcta cgaagagatg
     7501 gccgccgagg cggagaagac gcgcaagctc gatttcgagg agattcagat cccggtcgga
     7561 ccgcgactgg gcaacgtcgt cgtcgacgtc caacacctcg acaagggcta cgacgggcgc
     7621 accctcatca aggacctgtc cttcaccctg ccgcgcaacg gcatcgtcgg ggtcatcggt
     7681 cccaacgggg tcggcaagac cacgctgttc aagaccatcg tcgggctcga gcagccggac
     7741 agcggcaccg tcaaggtcgg agagaccgtc aagctcagct acgtagacca ggcccgcgcg
     7801 ggcatcgatc caaagaagaa tgtgtggcaa gtcgtctcgg acgggctgga ctacatcgag
     7861 gtcggccgca ccgaagtccc gtcccgggcc tacgtgtcgg cgttcggttt caagggcccc
     7921 gaccagcaga aaccggccgg cgtgctgtcc ggtggggagc gcaacagact caacctcgcg
     7981 ctcacactca agcagggcgg caacctcatt ctgctggacg agccgaccaa cgacctcgac
     8041 gtcgagaccc tgagctcgtt ggagaacgcg ctggtgaact tcccgggctg cgcggtggtg
     8101 atttcgcacg atcgatggtt cctcgaccgc acttgcacgc acatcctggc ctgggagggc
     8161 gacgaggaca acgaggccaa atggttctgg tttgagggca acttcggcgc atacgaagag
     8221 aacaaagtcg aacggcttgg cgtcgatgcc gcccggccgc acagattgac acaccgcaag
     8281 ctcacgcggg actaatgtca gctctgctca tgctccgcga cgatgcggcc ggcacggcgg
     8341 cgaaggagtg gggccgatga agttagtggc acccgtcgtg agttgggagt aaccggcatg
     8401 acgcttgatc ccggagccaa gcacgatctc gagccgtgga cctcattcac cacgagggag
     8461 gacattcccg actggattgc gaatgcctac attcagagct atcggggtga ggaaccggga
     8521 gccgccgaca ccggtcccat cgacctgacc acgccggccg cgatggtgac gcccgcgatg
     8581 ctgcgtgcac actatcggct cggccagcgc cgcccggagt gcagaagctg tgtctgtgtc
     8641 tacccggcgg acgaccccgc cggattcggg cccgccctgc aggtcgtcac cgatcacggc
     8701 agcatgctgc tggattccgt cacggtgctg ctgcaccggc tcggcgtggc ctatgagggc
     8761 atcatgaccc ccgttttcga cgtgcatcgc acctcgacgg gcgacctggt cagcatcgaa
     8821 ccgaaatcgc ccggcacgtc gcaatacgac ggcgaggcct ggatctacgt ccagctgtcc
     8881 cgttccgtcg aaaccaaggc cttggccgaa gtcgaacggc tgctgcccaa ggtcctgacc
     8941 gacgtcgaac aggtcgccag cgacgctgcg acgatgttca ccaccctcag cgatctggcc
     9001 gccgccgtcg acgccaacgc gcaaggccat ttttcggcgc cggaccgtca ggacgtcgcg
     9061 gacctgctgg gctggctggg taacgggaat ttcctgctgt tgggctacca acattgccgg
     9121 gtgcgcgacg gtcaggtctc cggtgacgga tcgagcagcc tgggcgtcct gcgcacccgc
     9181 actggttcgc gcccccgatt gaccgacgac gagaaaatgc tggtgttggc ccaatccgtt
     9241 gtcggcagtt acctgcgcta cggcgcctac ccctatgtgg tagcggtccg cgaaaaggac
     9301 gacgagggcg tgatcgagca ccgcttcgtc gggctcttca ccatcgccgc catgaatgcg
     9361 gacgtcctgg aaatcccgat gattgcgcgc cgggtccacg aggcgctcgc gatggcggag
     9421 agcgacccca tccacccggg ccagctgctg ctcgacgtca tccagaccgt cccccgctcg
     9481 gagctgttct cgctgagcgc cgaacgactt ctgacgatgg ccaaagccgt ggtggatctg
     9541 ggatcacaac ggcgggcgtt gctgttcctg cgcgccgacc ggctgcagta cttcgtctcc
     9601 tgtttggtgt acctgccacg cgatcgctac accaccccgg tgcggctgcg gatcgaggac
     9661 atcctcgtcc gcgagttcgg tgggacgcga ctggagttcg ccgcccgcgt cagtgaatca
     9721 ccctgggcgc tcatgcattt catggttcgc ctgccccaga aagacagcgc tgccccggcc
     9781 gtcgcaccgg tcgacgtctc ggaagccaac cgaatccgga tccaggcatt gctcagcgag
     9841 gccgcgcgca cctggggcga ccgcctgatc gccgcggcaa accgctccgg gaccgtcggg
     9901 cacgccgacg ccgagcatta cgcgtccgcc ttccccgagg tctacaaaca ggccgtcaca
     9961 cccgacgacg cgatcaagca catcggcatc atcaacgggg tctccgacaa ttcggtcaag
    10021 ttggtgttgt ccgagcccgg tgcggacggg gtcgaccagc tgacctggtt cgtgggtggg
    10081 cgcaccgcgt cgttgagcca gttgctgccg atgctgcaga gcatgggcgt cgtcgtgctc
    10141 gaggagcgac cgttcaccgt cacccggccg gacggcctgc cggtgtggat ctaccagttc
    10201 aagatctcac cgcatcccac catcccgccg gccgcgacgg cggccgaacg ggatgcgacc
    10261 gcagagcgat tcgcggacgc ggtcaccgcg atctggcagg gccgcgtcga gatcgaccgg
    10321 ttcaacgaac tggtgatgcg tgcgaggctg acctggcagc aggtcgtgct gctgcgcgcc
    10381 tacgcgaaat atctgcggca ggccggcttt ccctacagcc agtcctacat cgaatcggtg
    10441 ctcaacgagc atccgtccac cgtgcggtca ctggtcacat tgttcgaagc gttgttcgac
    10501 cccaggtcat cgggctcgcc gaccggccgg gacgcgcaag ccgccgcggc cgccgtcgcc
    10561 accgacatcg atgcgctggt gagcttggac accgaccgca tcctgcgtgc gttcgcgtcg
    10621 ttggtgcagg ccaccttgcg taccaattac tttgtcacgc gtccgggttc ggcccgggcc
    10681 cgcgacgtcc tgtcgatcaa gctggacgcc cagctggtcg acgagctccc gctgccgcgc
    10741 cccaagtacg agatcttcgt ctactcgccc cgcgtcgaag gcgtgcacct gaggttcggc
    10801 ccggtggcgc gtggcggcct gcgctggtcg gacgacgcga cgatttccgc accgagatcc
    10861 tcggtctggt caaggcgcaa gcggtgaaga acgccgtcat cgtgccggtc ggggccaagg
    10921 gcggattcgt cctcaagcgt ccgccgctgc cgtccggcga ccccgccgtc gaccgcgacg
    10981 cctcccgcac cgaaggcgtc gcctgctacc agctcttcat ctcggggctg ctcgacgtca
    11041 ccgacaacgt cgaccacgcc accggaaagg tcagcccacc acccgaggtg attcggcgcg
    11101 acggcgacga cgcctacctg gtggtggccg ccgacaaagg caccgccacc ttctccgaca
    11161 tcgccaacga cgtcgccaag tcctacgggt tctggctcgg cgacgcattc gcgtccggcg
    11221 ggtcggtggg ctatgaccac aaggccatgg gcatcaccgc caagggcgcg tgggaggccg
    11281 tcaaacgaca cttccgggag atgggcgtcg acacccagac cgaggccttc accgtcgtcg
    11341 gcgtcggcga catgagtggc gacgtgttcg gcaacggcat gctgctgagc aagcacatca
    11401 ggctgatcgc cgccttcgac caccggcaca tcttcctgga cccggacccc gacgccgcgg
    11461 cttcgtggcg ggaacgccag cggatgttcg acctgccgcg atccagctgg gacgactacg
    11521 acaagtcgct gatcagtgag ggcggcggcg tttacagccg cgagcacaag gccatcccgg
    11581 tcagcccgca ggtgtgccac gccctcggca tcgacggcgc cggcgacggg gtcagcgaaa
    11641 tggccccgcc ggagctgatc aaggcgatcc tgcaggcacc ggtcgatctg ctgttcaacg
    11701 gcggcatcgg cacttacatc aaggccgagt cggaatccga cgccgacgtc ggagatcgcg
    11761 ccaacgatac ggtgcgggtc aacggaaatc aggtgcgcgc caaggtaatc ggcgagggcg
    11821 gcaatctcgg ggtgaccgcg cggggccgcg tcgagttcga tctgtcgggc ggccggatca
    11881 acaccgacgc gatggacaac tccgccggcg tggactgttc ggaccacgaa gtcaacatca
    11941 agatcctgat cgactcgctg gtcaccgccg gcaaggtgaa ggcggaagac cgcaaacagc
    12001 tgctggagtc gatgaccgat gaggtcgcgc aattggtgct caccgacaac gaggaccaga
    12061 acgatctgat cggcaccagc cgcgccaacg cggccagcct gctcccggtg cacgcgatgc
    12121 agatcaagta cctcgaagac cgtggggtga accgcgaatt ggaggcgctg ccgtcggaga
    12181 aggagattca ccggcgcacc gatatcggca tcggcctcac ctctccggaa ctgtcgacgt
    12241 tgatggcgca cgtcaagctg gcgctcaaag aggacatgtt gaccaccgag ctgccggagc
    12301 aggatgtttt cgcgtcccgg ttgccgctgt acttcccgac gccactgcgc gagcggttca
    12361 ccccggagat ccgcacgcac cagctgcgcc gtgaaatcgt caccaccatg ctggtcaacg
    12421 atctggtcga caccgcgggc atcagctacg cgttccggat cgcccaagac gtgggtgtcg
    12481 gttcgatcga cgcggtgcgc acctacgtgg ccaccgacgc catcttcggt gtgggccaga
    12541 tctggcggga gatccgcgcc gccaacgttc ccgtcgcgct gtcggaccgg ctcacgctgg
    12601 atacccgacg gctgatcgac cgcgccggac gctggctgct caactaccgg ccgcagccct
    12661 tggccgtcgg cgccgaaatc aatcggttcg ccgccaaggt caaagatctg acgccgcgga
    12721 tgtcggagtg gctgcgtggt gacgacaaag ccatcgtcga acaggaagcc gcggagttcg
    12781 cgtcgcaagg cgccccgaaa gagttggcct acatcgtcgc cgccgggctc taccgttaca
    12841 gcctgctcga catcatcgac gtcggtgaca tcaccgaaat cgacgccgcc gaagtggcag
    12901 atacctattt cgccctgatg gaccggctgg gcaccgacag cctgctgacg gcggtatcgg
    12961 agcttccacg caaagatcgc tggcactcgt tggcgcgctt ggcgattcgc gacgacatct
    13021 actcctcgct gcggtcgctg tgcttcgacg tgctcgcggt cggagagccc gacgaaagcg
    13081 gcgaagaaaa gatcgccgag tgggagcaca tcagcgcgtc gcgggtggag cgggcccgca
    13141 gcacgctcag cgagatacga gaaagcgggg caacggatct cgcgacgctg tcggtggccg
    13201 cgcgacagat ccggcgcatg acccgcacca gcggaagggg atcgtcgagt tgagcacggg
    13261 gttcgtcgcg cccgtgccgg tgcgttggtc ggatatcgac atgtaccagc acatcaacca
    13321 cgcgaccatg gtcacgattc tcgaagaggc acgcgtgccg ttcctcaagg aacctttcgc
    13381 ggccgacatc accaccatcg ggttgctgat cgccgacgtc cgggttactt acaaggatca
    13441 gttgcggctg gcggattcgc cgctgcaggt caccatgtgg accaaacggt tgcgggcggt
    13501 cgacttcacg ctggggtacg aggtgcgctc ggccaactcg cgtcccgagt ccaagcccgc
    13561 cgtcgtcgcc gagtcgcagc tggccgcggt ccacatcgag gagcagcggc tggtgcgact
    13621 gtcaccacac catcgggagt atctgcaacg gtgggtccgc gaataaacgg gatggccgaa
    13681 cgcggtctat ggctgggccc cgacggcagg gcggcccacc gcgcagacct ggccacattc
    13741 gtcgatcacg cgatgcggct cgatgacgcc gcggtcgtgc ggctgcgcgc tcgatcggcc
    13801 ggactgattt cggcttgggt ggcaacgggt ttcgacgtgt tggccagccg cgtggtcgtc
    13861 ggcaaagtgc ggcccgacga tctgtcggtg ggggcggatg cgctggcccg cggcctgtcc
    13921 gcgatggacg cctcgggcta cgtcgatccg ggcttctcga tggacgctgc gtggcggggc
    13981 gcattgcccc cggaatcggg cttcacccac ctcgacgacg tacccgcacg ggtcatgctg
    14041 gacctggcgc agcgtggcgc gcgactcgcc agggaacacg gcagttccca cggcccgccg
    14101 gtctcgctgc tcgatcaaga ggtcattcgg gtcagctcgg ccgacatcag cgtcgggctc
    14161 ccgatgcgct gcgtgtttgc tttgaccgca atgggtttcc tgccgcaatc gcccgacgcg
    14221 atcgccgccg acgagatggt ccgggtgcga atactgccga gctggctgcg ggtagacgcc
    14281 aggttcggct cggtgtaccg ccgccgcagc catgcggcgc tggtgctccg ttgatccggc
    14341 tcagatcagc caggccgccg cgtccggtgg caatttgccg ccgtccaacg ggccgctggc
    14401 caggatgact tctcccgccg gcaaagccat cgggtgggat cccgcattca gcgcacacac
    14461 cagacccccg ccgcgcccga acatcagcgc atcgggcggc gcggtctgcc attgtaagtc
    14521 gccgtcgaat tcgggccgct gcctgcgcaa ttcgatggcg cgctggaaga aagacaaggt
    14581 cgacgccgga tccgcgctct gcttttccac ggtcagcgcc gcccaatccg cgggcatggg
    14641 caaccaggtg tcggcggtcg tggagaaccc gaacggggga gtgtcgcccg accacggaat
    14701 cggaacccgg cacttgtcgc ggccgcgttc ggtatgcccc gagcgttccc acgtcggatc
    14761 ctgcagcacg tcttcgggca gatccaacac atcgggcaag cccaactcct cgccgttgta
    14821 gatgaagacc gcacccggca gggcgagcat cagcatcgcc aaggcacgcg cgcggcgcag
    14881 cccgatctcg ccgccgccgt agcgggtgac ctcgcggccc acgtcgtggt tggacagcgt
    14941 ccaggtcggg atcgcgtttt cgatcgccgt ggccgtcagc gaattctgaa ttgcgtcatg
    15001 gatttcggtc gcgtcgaagc cgatcttcgt cagccggaaa ttgaagccga gatgcagttc
    15061 gtcgggccgc agatattccg cccagaggag gttgtccatg acccagacct cgccgatggt
    15121 gaccgccccg cgatagtcgt cgacgatctt gcggatcccg cgatggatct cgtggacgct
    15181 cgggtggttg aaacgcgggt cgtcgtcgct gtggctcaac accttcacgt cgtccttcgc
    15241 gtccggcagt ccgggcggct tggccatgcc atgcgccacg tcgatgcgga agccgtcgac
    15301 accgcgttcc agccagaagc gcagcgtctt ctcgaagtcg tcgaagacct cggtgttgtc
    15361 ccagttcaga tccggctgct cggcgtcgaa caggtgcaga taccactggc cggggttgcc
    15421 gtcgggttcg gccacccgtg tccaggccga cccgccgaac accgacgtcc aattgttggg
    15481 cggtgactcc ccgccgggac cgcgtccgtc gcgaaagatg tagcgatccc gcgcgtcgcc
    15541 gcccgggccg gcggccagcg cggcctgaaa ccacgggtgc gccgagctgg tgtggttggg
    15601 caccacgtcc atggtgatct tgatgccacg ctcctgggcc gcggcgatca gccgctcgat
    15661 cgcggccatc ccgccgaaca gcggatcgat gtcgcgcgga tccgcgacgt cgtagccgtg
    15721 gtcggccatc ggtgacacgg tgaccgggtt gagccaaatc gagtcgacgc caagcttttc
    15781 caggtacccc agccgggccg tcaagccatc caggtcgccg acgccgtcgc cgttgctgtc
    15841 ggcgaacgag cgcggataca cctggtagaa cacggcattc gaccaccatg ccggccccgg
    15901 tcggggagcg cgtgcgcgtg gagtgccggg ttccatcgaa ctagcgccgg ggctcatgtt
    15961 gctccgttcc gttttggctg gacatcaaaa cgcggaatta accagggagt gggcggccat
    16021 ctcaagatag tcgagcaact cgcgccggtg ctcgtcgtcg agggtattcg agtcgatcga
    16081 ggcgacggcg gtgtgcatgc accgcagcca ggcgtcacgc tcgatgggcg tgatccggaa
    16141 cggggcgtgg cgcatccgca gccgcggatg gccgcgctgg tccgagtagg tccgcgggcc
    16201 gccccagtac tgctcgagga acatccgcaa ccgctcctcg gcggcggcga ggtcgtccgc
    16261 gggatagagc tggctgagaa tctcgtcctc cgggacctgc gcgtagaagt gcgacacgat
    16321 cgcggcgaag gtttcggcgc cgccgaccgc gtcgtaaaag ctctcctgat ccattgcctc
    16381 cattgtggtg catcgcccgc ggcggcacgc ggagacaggc tcgtcatctt tgcgcgcgcg
    16441 ccttctgttc accggctcga catggcgcgc accagcaata ggcgtgcgat tggcggcgaa
    16501 tcatggtgga ctatccgcgg aggatctatg gcgcatggga gcagacgccg cagccaccgc
    16561 agtcccggtg ccgcggcagg gataaccggg cccgcaaccg cgtcatctct gcacagcgtt
    16621 gagatctatc cacgcgttga gaattatgca cgcgtcgaga cccatccgcc cggccgtcac
    16681 gaggcggcat cgatctggag tcgccggcgg gtgctgctgc tgaactccac ctacgagccg
    16741 ctgaccgcgc taccgatgcg gcgcgccatc gtcatggtga tctgcggtaa ggccgacgtc
    16801 gtgcaccacg accccgccgg accggtcatt cactcggcca ccagctcgat cgtggttccg
    16861 tcggtgatcc agctgcggt
//
LOCUS       DASSGQ010000297        10727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1510156,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000297 DASSGQ010000000
VERSION     DASSGQ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10727
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510156"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VFI55_13145"
     CDS             <1..474
                     /locus_tag="VFI55_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET7075012.1"
                     /translation="DTPGFLPGAEQEKNGIIKRGGRFLSAVVEADVPKVTITVRKSYG
                     GAYAVMGSRQLTADFNFAWPTARIAVIGAEGAAQLLMKRFPDPTTPEAQQIKKDFIEG
                     YNLNMAIPWIAAERGFIDAVIDPQQTRLLIRKSLHLLRDKQLWFRVGRKHSLLPL"
     gene            complement(540..2339)
                     /locus_tag="VFI55_13150"
     CDS             complement(540..2339)
                     /locus_tag="VFI55_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7075013.1"
                     /translation="MSEDHPQYGFESVDSLPFSTPEKSQRYQTADYRGAVGLNWYRTD
                     PTLQFAMAYYLQPDELALAEPHLTRMGELMGGPVARWAEETDRNPPRLERYDRWGHDI
                     SQVVMPSSFRQSKRVILDAQQQLRTEARAAKVNSSLAMFASNYLLNQADIGMGCALGT
                     GGGMVQSLVAAYAPADVREHVLAKFASGEWAGETAQLLTERTGGSDLGALETTATRSS
                     DSWLLNGFKWFASNCAGEAFVVLAKPEGAPDSSRGVANFLVLRTRRDGSRNGVRVRRL
                     KDKLGTRSVASGEVEFVDAEAFLLSDEPSGPQADAGASDGKGLGRMMELTNAARLGIA
                     LFGLGNARRALVESLCYAGRRHAFGGALIDKPLMRRKLAEMIVDVEAAQALVFDGTGA
                     INHRQPRGIRQRIAVPVTKLRVCRLGITAASDAIEIHGGNGYIETWPVARLLRDAQVN
                     TIWEGPDNILCLDVRRGIEKTRAHEPLLARLRDAVSVSDHDETTDLVSRRIEDLDAAI
                     TAWAKLDGAVAEARLFPLAQFMGDVYAGALLTEQAAWERATRGGERKALVARLYAQRY
                     LADRGPLRGIDADSDEALDRFDELVDGAFTPRV"
     gene            complement(2380..2763)
                     /locus_tag="VFI55_13155"
     CDS             complement(2380..2763)
                     /locus_tag="VFI55_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET7075014.1"
                     /translation="MRADEFDTSAATADAAVDETETLAGGIGMLVVKRGPNAGSRFLL
                     GQPVMSAGRHPASDIFLDDITVSRRHAEFRVEKGKFLVVDLGSLNKTYLNREPVDSAV
                     LVHGDEIQLGNFRLVFITGPADPRN"
     gene            complement(3193..3708)
                     /locus_tag="VFI55_13160"
     CDS             complement(3193..3708)
                     /locus_tag="VFI55_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075015.1"
                     /translation="MDTLTRLGTCAAAITVAISPATISATSGIDTAAADVATRVPLDN
                     DFRRCDFSLVTPTPPVQDDNLGSASALIHTAGSQAVAEVHLVDAPDPGTHFDVGLIQE
                     PRPASATCGHGDPGTAFTGIDTDAAGNATVTIQDTIRQGTTGVWVIIERPNPISQNPA
                     EFYTSDFVAPV"
     gene            complement(3907..7146)
                     /locus_tag="VFI55_13165"
     CDS             complement(3907..7146)
                     /locus_tag="VFI55_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052636 - arabinosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071766 - Actinobacterium-type cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosyltransferase domain-containing protein"
                     /protein_id="HET7075016.1"
                     /translation="MTVTTVRNSEDKAAPRQDVRVTRWVATIAGLIGFVLSVATPLLP
                     VVQTTATLNWPQNGQLNSVTTPLISLTPVDVTVTVPCSVLRALPPDGGVVLSTAPKKG
                     KDAALNGLFVVANGQRVDVTDRNVVIASAPREQAAGGAGAPGCSSIEITSTQAGTFAT
                     FVGLTDPTGKPVSGGFPDPNLRPQIVGVFTDLAGPAPPGLKLSATIDTRFSTTPTTLK
                     LVAMVLAIVSTIVALVALWRLDQLDGRRMHRWIPTRWRKFTLVDATVIFGFLLWHLIG
                     ANSSDDGYILGMARVADRAGYMSNYFRWFGSPEDPFGWYYNLLALMTHVTDASLWMRL
                     PDLVAGLVCWLLLSREVLPRLGPAVTSSTAANWAAAMVLLTAWMPFDNGLRPEPIIAL
                     GSLVTYVLIERSMCASRLTPAALAVITAAFTLGVQPTGLIAVAALVAGGRPILRILVR
                     RHRLVGTWPLVAPMLAAGSVILTVVFADQTLSTVLEATRIRTDIGPSQAWYTENLRYY
                     YLILPTVDGSLSRRFGFLITALCLFTALFIMLRRKRVPGVARGPAWRLMGVIFATMFF
                     LMFTPTKWVHHFGLFAAVGAAMAALTTVLVSPAVLRWSRNRMAFLAAVLFLLALCFAT
                     TNGWWYVSSYGVPFNNTVPKIAGITISTIFFALFAIAALWAIWLHFASRDHGEGRLAR
                     AVTAAPVPIAAGFMALVFIASMVAGIVRQYPTYSNGWANLREFAGGCGLADDVLVEPD
                     SNAGFMAPLPDNFGPLGPLGGVNPIGFTPNGVPERTLAESVKETSVPQPGTDYDWDAP
                     TKLTAPGVNGSTVPLPYGLNPDQVPLAGSYTTGPQQQSRLTSAWYQLPKPDAGHPLVV
                     VTAAGTIAGNSILHGHTPGQTVVLEYGRPGPGGEVVPAGRQVPYDLYGEQPKVWRNLR
                     FVRSQMPADAVAVRVVAEDLSLTPEDWIALTPPRVPELRSLQEYVGSSQPVLLDWAVG
                     LAFPCQHPMLHVDGVTEIPKFRITPDYNAKKQDTDTWQDGVNGGLLGITDLLLRAHVM
                     STYLSRDWGRDWGSLRKFETVADAGTAQLELGTATRTGWWSPGEIRIKP"
     gene            complement(7143..10481)
                     /locus_tag="VFI55_13170"
     CDS             complement(7143..10481)
                     /locus_tag="VFI55_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052636 - arabinosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071766 - Actinobacterium-type cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosyltransferase domain-containing protein"
                     /protein_id="HET7075017.1"
                     /translation="MPPDGKQRSQRIPRLIAVVAGTVGLLLCGSVPLLPVKQTTATIL
                     WPQGTADGHVTQVTAPLVSGAPRALDISIPCPAMATLPPDGGLVVSTLPTGGVDTGKN
                     GLFVRADKNVIVVAFRDSVAAVAQRSAVAAGACSVLHTWADAGGAGADFVGIPGASGM
                     LSAEKKPQFGGIFTDLKVPAQPGLSARVDIDTRFILAPSAIKKVVMVVGGLAVLAALL
                     AMAALDRRSRGDDMLVNWRSPIAWLSRYRPGVRRASWRRVIAATWAADLGVIATLLLW
                     HVIGATSSDDGYNLTIARIAPKAGYVANYYRYFGTTEAPFDWYLAVLAKLAAVSTAGV
                     WMRLPATLAGIVCWLIISHCVLRRLGPGKGGLAANGAAVFTAGAVFLAAWLPFNNGLR
                     PEPLIALGVMITWMLVERAIALQRLVPAAVAIIVAMLSAALAPQGLIAVAALLTGARA
                     IAQTIRRRRPTDGLLPALLVLAASLSLIFVVVFRSQTLATVAESARIKYKVGPTIAWY
                     QDFLRYYFLTVESNAEGSMSRRFAVLVLLLCMFGMLVVLLRRGRVPGLASGPAWRLIG
                     TTAIGLLLLTFTPTKWAVQFGAFAGLAGALGAVTAFTFARIGLHSRRNLTLYITALLF
                     VLAWATSGVNGWFYVGNYGVPWFDIQPVIASHPVTSMFLTLSILTGLLAAWQHFRMDY
                     AGHTEVKDNRRNRVLASTPLLVVATIMVLGEVGSLAKGAVGRYPAYTTARANVAALLS
                     SGGSPSCAMGDDVLTEPDPNAGLLQPAPGQQFGPAGPLGGVNPVGFKPDGVGDDLRSY
                     PVVTKPGVVNSDASPNKPNATMSDSAGTAGGKGPAGVNGSHAALPFGLDPARTPVMGS
                     YGENSLAATATSAWYQLPANWKESTPDRPLVVVSATGAIWSYKEDGTFTYGQQLKLQW
                     GVTRADGTTQPLAQVQPIDIGPEPAWRNLRFPLAWAPPEANVARIVAYDPNLSSEQWF
                     AFTPPRVPLLQTLQQLIGSHTPVLMDIATAANFPCQQPFPEHLGVAELPRYRILPDHK
                     QTAASSNGWEASEAGGPFLFTQALLRTSTISTYLRGDWYRDWGSVEEYHPLVPADQAP
                     NAVIEQGVITVHGWSRQGPIRALP"
BASE COUNT         1769 a         3540 c         3696 g         1722 t
ORIGIN      
        1 gacacgcccg gattcctgcc gggggccgag caggagaaga acggcatcat caagcgcggc
       61 ggacggttcc tttccgccgt cgtcgaagcc gacgtgccaa aggtgacgat caccgttcgc
      121 aagtcctacg gcggcgcgta cgcggtgatg ggttcccggc agttgaccgc cgacttcaac
      181 ttcgcctggc cgaccgcgcg catcgcggtg atcggtgccg agggtgccgc gcagctgctg
      241 atgaagcgct tcccggaccc gaccacgccc gaggcgcagc agatcaagaa agacttcatc
      301 gagggctaca acctgaacat ggcgatcccg tggatcgccg ccgagcgcgg gttcatcgac
      361 gcggtcatcg acccgcagca gacccggcta ctgatccgta agtccctgca tttgttgcga
      421 gacaagcagt tgtggttccg cgtcggacgc aagcacagcc tgctcccgct ctagggcgtc
      481 gctgtccttc gccgagtgtg aattgggcga cgctacacgc cgattcggcg tcggctaatt
      541 cacactcgag gcgtgaatgc gccgtcgacg agctcgtcga accgatctag cgcctcgtcg
      601 gagtcggcgt cgatgccgcg cagcggcccg cgatcggcga gataacgctg cgcgtacagc
      661 cgggcgacga gtgccttgcg ctcaccgccg cgcgtcgccc gctcccaggc ggcctgctcg
      721 gtgagcagcg cgcccgcgta cacgtcgccc atgaactggg ccagcgggaa cagccgcgcc
      781 tcggccaccg cgccgtcgag tttggcccag gccgtgatcg ccgcgtcgag atcctcgatg
      841 cgcctggaga ccaggtcggt ggtctcgtcg tgatcggaca ccgacaccgc gtcgcgcagg
      901 cgcgccagca gcggctcgtg ggcgcgggtc ttctcgatgc cgcgccgcac gtcgaggcac
      961 aggatgttgt cggggccttc ccagatggtg ttgacctgcg cgtcgcgcag tagccgggcc
     1021 accggccatg tctcgatgta gccgttgccg ccgtggattt cgatcgcgtc cgacgccgcg
     1081 gtgatcccga gccggcacac cctgagcttg gtgacaggta ccgcgatgcg ttgccggatg
     1141 ccgcggggct gccgatggtt gatggcgccg gtcccgtcga aaaccagcgc ttgggcggcc
     1201 tcgacgtcga cgatcatctc cgcgagcttg cggcgcatca gcggcttgtc gatgagcgcg
     1261 ccgccgaacg cgtgccgccg tccggcgtaa cacagcgact cgaccagggc gcgacgggcg
     1321 ttgccgagcc cgaacagggc gataccgagc cgcgcggcgt tggtcagctc catcatgcgg
     1381 ccgagtccct tgccgtccga cgcgccggcg tcagcttggg gaccgctggg ttcgtcggac
     1441 agcaggaacg cctcggcgtc cacgaactcg acctcgccgg aggccaccga gcgggtgccg
     1501 agcttgtcct tcaaacggcg cacccgcacc ccgttgcgcg aaccgtcgcg acgggtgcgg
     1561 agcaccagga aattggcgac gccacggctc gagtcggggg cgccttcggg tttggccagc
     1621 acgacgaacg cctccccggc gcagttggag gcgaaccact tgaagccatt cagcagccac
     1681 gagtcgctgc ttcgtgtcgc cgtcgtctcg agagcgccca agtcggagcc gccggtgcgt
     1741 tcggtcagca actgcgccgt ctcgccggcc cactcgccgg acgcgaactt ggccagcacg
     1801 tgctctcgca cgtcggccgg cgcgtaggcc gccaccagcg actggaccat gccgccgccc
     1861 gtgccgagtg cgcagcccat cccgatgtcg gcctggttga gcaggtagtt cgacgcgaac
     1921 atcgccagcg acgagttcac cttcgcggct cgggcttcgg tgcgcagctg ctgctgggcg
     1981 tccaggatga cgcgcttgga ctgcctgaac gaggacggca tcacgacctg gctgatgtcg
     2041 tgtccccacc ggtcgtagcg ctccagccgg ggcgggttgc ggtctgtctc ctcggcccat
     2101 cgcgccaccg ggccgcccat cagctcgccc atgcgcgtca agtggggctc ggccagcgcc
     2161 agctcgtcgg gctgcaggta gtaggccatg gcgaattgca gggtgggatc ggtgcggtac
     2221 cagttgagcc cgacggcgcc gcgataatcc gccgtctggt agcgctgtga cttttccggg
     2281 gtggagaagg gcaggctgtc gaccgactcg aacccgtatt gggggtggtc ctcgctcatg
     2341 tcgcgacggt attacaaccg aagcgcccgg tgaacacgat cagttccgtg ggtccgccgg
     2401 tccggtgatg aacaccaggc ggaagtttcc gagctggatc tcatcgccgt ggaccagtac
     2461 cgcggagtcg acgggctcac ggttgaggta ggtcttgttc aggctgccca ggtcgacgac
     2521 gaggaatttg cctttctcca cgcggaattc ggcgtgcctg cggctcacgg tgatgtcgtc
     2581 gaggaagatg tcgctggccg gatgccggcc ggccgacatg acgggctggc ccaataggaa
     2641 ccgcgacccg gcgttgggac cccgcttcac gacgagcatc ccgatgccgc ccgccagcgt
     2701 ttccgtctcg tccactgccg cgtctgctgt agccgctgac gtgtcgaact cgtcagcgcg
     2761 catcgccccc gtgatctcgt tgggcgtctg gctgtgctcg ttcacctgct gccggtgtgt
     2821 cgacacgtcg gctcaatcac atggcccgca gaagataccg ggaatggcgc gcgagaaagc
     2881 atgcgcgtcc tgggctgcgc ccgttgtgaa gcgtccatcg ctgcatcgga ccattccatc
     2941 gaggtgccga ggtggggacc caagtcccgt ccgctcggtg tgttcgtcct tggcgcttgt
     3001 cgtcgattcc agccgccgct gacctgtgcg tttcctctcc ggctgacggt agcgcatgat
     3061 cgctgcacag ccaacgatcg tgattcgctc gtgacttaag gcccggaccg cgcggccccg
     3121 tacggcaatg cgcacgcagg tgtcaacggt gtttccgggg gcccacgccg ggcatcgaat
     3181 agctcgtacg gcttataccg gagccacaaa gtccgatgtg tagaactcgg ccggattttg
     3241 cgagatcggg ttcggacgct cgattatcac ccagacgcca gtagtgccct ggcgaatggt
     3301 gtcttgaatt gtgactgttg cgttgccggc cgcgtcggtg tctattccgg tgaatgcggt
     3361 gcccggatcg ccgtgaccgc acgtcgccga tgccggacgg ggttcctgga tgaggccgac
     3421 gtcgaaatgg gtgcccgggt cgggagcgtc gaccagatgc acctccgcga ccgcttgtga
     3481 tccggcggtg tggatcaggg ccgaggctga gcccagattg tcgtcctgta ctggcggcgt
     3541 cggggtgacg aggctgaaat cgcagcgtcg gaaatcattg tccagcggga cccgcgtggc
     3601 gacgtcagcc gcggccgtgt cgattcccga tgtcgcagat atcgtagccg gtgagatcgc
     3661 tacggtgatc gccgccgcgc aagtgcctaa ccgtgtcagt gtgtccatcg agccaccttt
     3721 ttaccctttt gttaattccc accctaggga attaggtaat gacccgaatt cgccgtgagt
     3781 aaacatttgg tggcaaatta gtcagacgtc aaattgtggc tgttatttga ctgatggcga
     3841 attacgctgg cgcggattat tgaaatccgt gtgatacaag ctttttcgcg tattgcgcgc
     3901 ttcgtatcag ggtttgatcc ggatctctcc gggtgaccac catccggtgc gggtcgcggt
     3961 gcccagttcg agctgcgcgg tcccggcgtc ggcaacggtt tcgaacttgc gcagcgatcc
     4021 ccagtcacgg ccccagtcgc gggagaggta cgtggacatc acgtgggcgc gcagcagcaa
     4081 gtcggtaatg cccagcaggc cgccgttgac gccgtcctgc caggtgtcgg tgtcctgctt
     4141 cttggcgttg tagtccggcg tgatccggaa cttcgggatc tcggtgacac cgtcgacgtg
     4201 caacatgggg tgctggcacg ggaatgccag cccgaccgcc cagtccagca ggacgggctg
     4261 cgacgagccg acgtactcct gcagcgagcg cagttccggc actcgcggcg gggttagcgc
     4321 aatccagtcc tccggcgtca gggacaggtc ctcggccacc acccggaccg cgaccgcgtc
     4381 cgccggcatt tgggaccgga cgaagcgcag attgcgccac accttcggct gctccccgta
     4441 caggtcgtag ggcacctgcc gcccagccgg gacgacctca ccgccgggac cgggcctgcc
     4501 gtattcgagg accacggtct gcccgggggt gtggccgtgc aggatgctgt tgcccgcgat
     4561 cgtgcccgcg gcggtcacca ccaccagggg atgcccggcg tccggcttgg gcagctggta
     4621 ccacgccgac gtcagcctgc tctgctgttg agggccggtc gtgtagctgc ccgccagcgg
     4681 aacctgatcc ggattgaggc cgtagggcag cggcaccgtc gatccgttga cgcccggcgc
     4741 cgtcagcttg gtcggcgcgt cccagtcgta gtcggtgccc ggctgcggca ccgacgtctc
     4801 cttgaccgac tcggccaggg tacgttccgg tacaccgttg ggggtgaatc caattgggtt
     4861 gaccccgcct agggggccca acgggccgaa attgtcgggc aggggcgcca tgaagccggc
     4921 gttgctgtcc ggctcgacga gcacgtcgtc ggccagtccg cagcccccgg cgaattcgcg
     4981 caagttggcc cagccattgg agtaggtggg gtactggcgc acgattccgg cgaccatcga
     5041 cgcgatgaac accagggcca tgaatccggc cgcgatcggc accggcgccg ccgtcactgc
     5101 ccgcgccagc cggccctcgc cgtggtcccg cgacgcgaag tgcaaccaaa tcgcccacag
     5161 cgccgcgatc gcgaacaagg cgaaaaatat tgtgctgatc gtgattccgg cgattttcgg
     5221 caccgtgttg ttgaacggga cgccgtagct cgagacatac caccagccgt tcgtggtggc
     5281 gaaacacagc gccagcaaga acaacaccgc cgccaggaac gccattcggt tgcgcgacca
     5341 gcgcagcacc gccggtgaca ccagcaccgt ggtcagcgcg gccatcgccg cacccaccgc
     5401 ggcgaacagc ccgaagtggt gcacccactt ggtcggggtg aacatcagga agaacatggt
     5461 ggcgaagatg acgcccatca gccgccacgc cggcccccgg gccacgccgg gaacccgctt
     5521 gcgccgcaac atgatgaaca acgcggtgaa caggcacagc gcggtgatca ggaaaccgaa
     5581 ccggcgcgac agtgagccgt cgacggtggg caggatgagg tagtagtagc gcagattttc
     5641 cgtgtaccag gcctgactgg gaccgatgtc ggtgcgaatc ctggtggctt ccaagaccgt
     5701 tgacagtgtc tggtcggcga aaaccacggt gaggatgacc gaaccggcgg ccagcatcgg
     5761 tgccaccagc ggccacgtgc cgaccaggcg atgacggcgc accaaaatcc gcaggatcgg
     5821 gcggccgccg gcgaccaggg cggcgaccgc gatcaggccg gtgggctgca cgcccagggt
     5881 gaatgccgcg gtgatgacgg ccagcgcggc cggggtcagc cgggacgcgc acatcgagcg
     5941 ctcgatcagc acataggtga ccagcgaccc cagcgcgatg atgggttcgg ggcgaaggcc
     6001 gttgtcgaaa ggcatccacg cggtgagcag gaccatggcg gccgcccagt tggcggcggt
     6061 gctggaggtc accgccggac ccagccgggg caacacctca cgcgacagca gcagccagca
     6121 caccagcccg gcaaccaggt cgggcaaccg catccacagg ctggcgtcgg tgacatgggt
     6181 catcagcgcc agcaggttgt agtaccagcc gaacgggtcc tccgggctgc cgaaccagcg
     6241 gaagtagttg gacatgtagc cggcacggtc agcgacccgg gccatgccca ggatgtagcc
     6301 gtcgtcggag gaattcgcgc cgatcagatg ccacagcaga aagccgaata tcaccgtcgc
     6361 gtcgaccagt gtgaatttgc gccagcgggt cggaatccac cggtgcattc ggcggccgtc
     6421 cagctggtcc aggcgccata gcgcgaccag cgcgacgatg gtggacacga tggccagcac
     6481 catcgcgacc agtttcagtg tcgtcggggt ggtggagaac cgggtgtcga tggtcgccga
     6541 cagcttcagt ccgggcggcg cgggcccggc cagatcggtg aacaccccga cgatttgggg
     6601 gcgcaggttg ggatcgggga aacccccgct gaccggcttg cccgtggggt cggtcaatcc
     6661 gacaaacgtg gcgaaggtgc cggcctgtgt ggaagtgatc tcgatgctgg aacatccggg
     6721 ggcacctgcg cccccggccg cctgctcccg aggcgcgctg gcgatcacca cgttgcggtc
     6781 ggtcacgtcg acgcgttggc cgttggcgac gacgaacaac ccgttcagcg cggcgtcctt
     6841 gcccttcttg ggtgccgtac tcagcacgac cccgccgtcg ggtggcaggg cacgtagcac
     6901 cgaacacggc acggttaccg tcacgtcgac cggtgtcagc gaaatcagcg gggtggtaac
     6961 gctgttcaat tggccgtttt gtggccagtt cagcgtcgcg gtggtctgca cgacgggcag
     7021 cagcggcgtc gcgaccgaca ggacgaaacc aatcagcccg gcgatcgtcg cgacccagcg
     7081 cgtcacccga acgtcttggc ggggcgccgc cttgtcttcg ctgttcctga cggtggtgac
     7141 ggtcatggga gtgcccgaat cggtccttgc cggctccagc cgtgcacggt gatcactcct
     7201 tgttcgatga cggcgttcgg agcctgatcg gccggcacca acgggtggta ctcctcgacc
     7261 gatccccagt cccgatacca gtcgccacgc aggtacgtcg agatcgtcga ggtgcgcagc
     7321 agcgcctggg tgaacaggaa cggaccgccg gcctcgctgg cctcccaccc gttcgacgac
     7381 gccgccgtct gtttgtgatc gggcaggatg cggtatcgcg gaagttcggc aacgccgagg
     7441 tgttcgggga acggctgctg gcaggggaag tttgcggcgg tcgcgatgtc catcagcacc
     7501 ggtgtgtgcg atccgatcaa ctgctgcagg gtctgcagca gcgggacccg cggcggcgtg
     7561 aacgcgaacc actgctcgga actcagattc gggtcgtaag cgacgatgcg tgccacgttg
     7621 gcctccgggg gcgcccaggc gagcgggaac cgcagattgc gccacgccgg ctccggcccg
     7681 atgtcgatcg gctggacctg cgcgagcggc tgggtggtgc cgtccgcgcg agtaacgccc
     7741 cactgcagtt tcagctgttg cccgtaggtg aaggtgccgt cctctttgta cgaccagatg
     7801 gcgccggtcg ccgagaccac caccagcggc cggtcggggg tgctctcttt ccaattggcg
     7861 ggcagctggt accacgccga ggtggcggtg gcggccagcg aattttcgcc gtagctgccc
     7921 atcaccggag tgcgggccgg gtcgaggccg aacggcagtg ccgcgtgcga cccgttcacc
     7981 ccggccggcc ctttcccgcc ggcggtgccc gcggagtcac tcatggtggc gttgggcttg
     8041 ttgggcgacg catcggaatt caccaccccc ggtttggtca ccaccgggta ggacctcagg
     8101 tcatcgccga ccccgtcggg tttgaagccg acggggttga ctcccccgag cgggcccgcg
     8161 gggccgaatt gctgtcccgg ggccggctgc agcagacccg cattggggtc tggctcggtc
     8221 agcacgtcat cgcccatcgc gcagctaggg gacccgcccg aggaaagcag tgccgccacg
     8281 ttggccctgg cggtggtgta ggccgggtag cggcccacgg cgcctttggc cagcgagccg
     8341 acctcgccga gcaccatgat cgtcgccacg accagcagcg gggtcgatgc cagaacgcgg
     8401 ttgcgccggt tgtcttttac ttcggtgtgc ccggcgtagt ccatccggaa gtgctgccag
     8461 gcggccagca gcccggtcag gatcgacagt gtcaggaaca tcgacgtcac cggatggctg
     8521 gcgatgaccg gttgaatatc gaaccacggc acaccgtagt tgcccacata gaaccagccg
     8581 ttgacgcccg aggtggccca cgccagcacg aacagcagcg ccgtgatgta cagcgtcaga
     8641 ttgcggcggc tgtgcaggcc gatccgggcg aaggtgaacg cggtgaccgc gccgagggcg
     8701 ccggccagcc cggcgaacgc accgaactgc acggcccact tcgtcggagt gaaggtcagc
     8761 agcagcaggc cgatcgccgt ggtgccgatc aagcgccacg ccggaccgct cgccagaccc
     8821 ggcaccctgc cgcgtcggag tagcaccacc agcatgccga acatgcacag cagcagcacc
     8881 agcaccgcga atcgccgcga catcgacccc tcggcgttgc tctccaccgt gaggaagtag
     8941 tagcgcagga agtcctggta ccacgcgatc gtcgggccga ccttgtactt gatgcgtgcc
     9001 gactcggcga ccgtggccag cgtttggctg cggaacacca cgacgaagat cagcgacaac
     9061 gacgctgcca gcaccagcag cgctggcagc agaccgtcgg tcggccggcg gcgccggatc
     9121 gtctgggcga tggcccgggc cccggtcaac agtgcggcga cggcgatcag cccctgcggg
     9181 gccagggccg cgctgagcat cgcgacgatg atcgcgaccg cggccgggac gagtcgttgc
     9241 agcgcgatgg cgcgctccac cagcatccac gtgatcatca cgccgagggc gatcagtggc
     9301 tcaggccgca ggccgttgtt gaacggcaac caggcggcca ggaacaccgc gcccgcggtg
     9361 aacaccgctg ccccgttggc cgccagcccg cccttcccgg ggcccagccg ccgcagcacg
     9421 caatggctga tgatcagcca gcacacgatt ccggccagcg tggcgggcag ccgcatccac
     9481 actcccgcgg tgctcaccgc cgccagcttg gccagcaccg cgagatacca gtcgaacggc
     9541 gcctcggtcg tgccgaagta gcggtagtag ttggcgacgt agccggcctt cggcgcgatc
     9601 cgggcgatgg tcaggttgta tccgtcgtcc gatgaggtcg cgccgatgac gtgccagagc
     9661 agcagcgtcg cgatgacgcc aaggtcggca gcccatgtcg cggcaatgac gcgccgccat
     9721 gaggcccggc gcacgccggg gcggtaccgg gacagccagg cgatcggcga ccgccagttg
     9781 accagcatgt cgtcgccgcg gctgcgtcga tccagggccg ccatcgcgag gagggcggca
     9841 aggaccgcga ggccgcctac caccatcacg accttcttga tggcgctggg cgccaggatg
     9901 aaccgggtgt cgatgtcgac acgcgccgac aaccctggct gagcaggcac cttcagatcg
     9961 gtgaagatgc cgccgaactg gggtttcttc tccgcggaaa gcattcccga ggcgccgggt
    10021 atgccgacga agtccgcgcc ggccccaccg gcgtcggccc aggtgtgcag gacgctgcac
    10081 gcgccggcgg ccaccgccga tcgctgcgcc accgcggcca ccgagtcccg gaacgcgacg
    10141 acgatcacgt tcttgtcggc gcgcacgaag agtccgtttt tgccggtgtc caccccgccg
    10201 gtcggcaggg tcgaaaccac caatccgccg tcgggaggca gggtcgccat cgcggggcat
    10261 gggatggaga tgtccagcgc gcgcggcgcc cccgacacca gtggggcggt gacctgggtg
    10321 acgtgcccat cggcggtgcc ctgcggccac aggatcgtcg cggtggtctg tttcaccggg
    10381 agcaagggaa cggacccgca caacagcagg cccaccgttc ccgcgacgac ggcgatcaac
    10441 cgcgggatcc gctgcgatcg ctgtttaccg tcggggggca cgaggctcga tggtaaggga
    10501 cgccgctttg tccggtgagg acgcagggcc gcgcagtggg cgtgcggccg atccggacag
    10561 gtcggattgg gtccggtgag gacgcagggc cgcgcagtgg gcgtgcggcc gatccggacg
    10621 ggtcggattg ggcgcggtga ggacgcaggg ccgcgtagcg ggcgtgcggc cgatccggac
    10681 cggtcggatt gggaaaacgg cgcctacgat gccggcgaat cgctgcg
//
LOCUS       DASSGQ010000298         3932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_599050,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000298 DASSGQ010000000
VERSION     DASSGQ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599050"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFI55_13175"
     CDS             <1..495
                     /locus_tag="VFI55_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET7075018.1"
                     /translation="AVAAAAGVDAALVHHYFGTKQQLFAAAIQIPIDPMTVLVPIREL
                     PIEDLGFALPSVLLPLWDSELGASFIATLRSLIAGADVSLARSFLEQVIVAEVAARVD
                     NPPGTGILRAQFFASQLMGVVMARYIVQIEPFASLPAEQIVATIGPNLQRYLTGDLPD
                     GLAP"
     gene            complement(461..676)
                     /locus_tag="VFI55_13180"
     CDS             complement(461..676)
                     /locus_tag="VFI55_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HET7075019.1"
                     /translation="MLDDSLLSILVCPADRGPLVLVDDELLYNPRLRRAYRIEDGIPV
                     LLIDEARDVDDDEHARLMERGRPANPQ"
     gene            complement(676..3696)
                     /locus_tag="VFI55_13185"
     CDS             complement(676..3696)
                     /locus_tag="VFI55_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HET7075020.1"
                     /translation="MAKPSRCAGQGAAVDLNFSMVTRPVERLIATAQNGLEVLRLGGL
                     ETGSVPSPSQIVESVPMYKLRRYFPPDSRPGQPPVGPPVLMVHPMMMSADMWDVTRED
                     GAVGILHANGLDPWVIDFGSPDKVEGGMRRTLADHIVALSQAVDTVKSETGNDVHLVG
                     YSQGGMFCYQAAAYRRSKNIASIVAFGSPVDTLAALPMGIPPNYAAPIADFMADHVFN
                     RLAIPSWLARMGFQMMDPLKTAKARVDFVRQLHDREALLPREQQRRFLESEGWIAWSG
                     PAISELLKQFIAHNRMMTGGFAINGQMVTLTDITCPILAFVGEVDDIGQPASVRGIRR
                     AAPNAEVFEYLIRTGHFGLVVGSRAAKESWPTVAEWVRWLSTGGDKPASIDLMADQPV
                     EHTDSGVAFSSRLAHGVGEVSEVALDIARGTANTVVAANKSMRTLAVETVRALPRLVR
                     LGQLNDHTRISLGRIIEEQAREAPHGEFLLFDGRVHTYEAVNRRVNNVVRGLIDVGVR
                     QGDRVGVLMETRPSALVAIAALSRLGAVAVVMRADADLAASVRLGGVTEILTDPTNLD
                     AARQLPGPVLVLGGGEARDLHLPEDATEHAQVVDMEQIDPDTVQLPAWYRPNPGLARD
                     LAFIAFSTAGGELVAKQITNYRWAVSAFGTASTAALDRRDTVYCLTPLHHESALLVSL
                     GGAVVGGARIALSRGLNRDRFAQEVRQYGVTVVSYTWAMLADIVDDPAFVLHGNHPVR
                     LFIGSGMPTGLWERVLDAFAPAHVVEFFATTDGQAVLANVSGAKIGSKGRPLPGVGRV
                     ELGAYDAEHDLILENERGFVQIAEPNQIGVLLAASKGPIDPTASVKRGVFAAADTWIS
                     TEYLFYRDDDGDYWLAGRRGSVVHTARGLVYPESVTDALGLINGVDLAVTYSVPVGGE
                     QLAVSAVTLLPGATVTAADLTDAVADIPIGLGPDIVHVCHEMTLSATYRPTASALRAS
                     GIPKAGRQAWYLDASSNEYRRLTAAVRAELRGAQ"
BASE COUNT          646 a         1316 c         1302 g          668 t
ORIGIN      
        1 gccgtcgccg cggccgccgg agtcgatgct gcactggtgc accactattt cgggaccaag
       61 caacagctgt tcgccgcggc gatccagatt ccgatcgacc cgatgacggt gctggtgcct
      121 atccgtgaac tcccgatcga agacctgggc ttcgcgctgc cgtcggtgtt gttgccgctg
      181 tgggattccg aactgggcgc gagtttcatc gcgacgttgc ggtcgctgat cgccggtgcc
      241 gatgtgagtt tggcgcgctc gttcctcgaa caggtgatcg tcgcggaagt ggccgcgcgc
      301 gtggacaatc cgcccgggac gggcatactg cgcgcccagt tcttcgcttc gcagttgatg
      361 ggcgtggtga tggcccgcta catcgtccag atcgagccgt tcgcatcgct gcctgccgaa
      421 cagatcgtgg cgaccattgg gcccaacctc cagcgctacc tcactgggga tttgccggac
      481 ggcctcgctc catgaggcgg gcatgctcgt cgtcgtcgac gtcgcgggcc tcgtcgatca
      541 gcagcaccgg gatgccgtcc tcgatgcgat aggcgcgccg cagccgcggg ttgtagagca
      601 gctcgtcatc caccagcacc agcggacccc ggtcggccgg acagaccagg atgctcagca
      661 gtgagtcgtc gagcatcact gcgcgccccg cagctcggcc cgcacggcag cagtcaggcg
      721 gcgatactca ttgctgctcg cgtcgagata ccacgcctga cgtcccgcct tgggaatccc
      781 cgatgcccgc agggcgctgg cggtgggacg gtacgtcgcg ctgagggtca tctcgtggca
      841 cacgtgcacg atgtcgggcc ccagcccgat cgggatgtcg gcgacggcat cggtcaggtc
      901 ggccgcggtc acggtggccc cgggcaggag cgtcaccgcc gataccgcca gctgctcgcc
      961 acccaccggc acgctatagg tcaccgcgag gtcgacgccg ttgatgaggc cgagcgcgtc
     1021 ggtgaccgat tcgggataga ccagcccgcg cgcggtgtgc accaccgagc cgcgccgacc
     1081 ggccagccaa tagtccccgt cgtcgtcgcg gtagaacagg tactcggtgg atatccaggt
     1141 gtcggcggca gcgaacactc cccgtttgac cgacgccgtc gggtcgatcg gccccttgga
     1201 cgcggcgagc agcaccccga tctggttggg ctcggcgatc tgcacgaaac cacgctcgtt
     1261 ctccaggatc aggtcatgct cggcgtcata ggcaccgagc tcaacccgcc caacgccggg
     1321 cagcggacgc cccttgctgc cgatcttggc gccggaaacg ttggccagca ccgcttgtcc
     1381 gtcggtggtg gcgaagaact cgacgacgtg agcgggcgcg aacgcgtcga gaacccgctc
     1441 ccacaatccg gtcggcatac ccgagccgat gaacagccgc accggatgat tgccgtgcag
     1501 cacgaatgcg gggtcgtcca cgatgtcggc gagcatggcc caggtgtagg acaccacggt
     1561 gacgccgtac tgccggacct cttgggcgaa ccggtcgcga ttcaggccgc gtgacaaggc
     1621 gatgcgggcg ccgccgacca cagcgccgcc caggctgacg agcagggcag actcgtggtg
     1681 cagcggcgtg aggcagtaca cggtgtcgcg gcggtcaagc gcggccgtcg aggcggtccc
     1741 gaaggccgac accgcccacc ggtagttggt gatctgcttg gccaccagct cgccaccggc
     1801 cgtgctgaac gcgatgaacg ccaggtcccg ggccagtccc gggttcggcc ggtaccacgc
     1861 gggcagctgg acggtgtccg ggtcgatctg ctccatgtcg acgacctggg catgctcagt
     1921 ggcgtcctcg ggtaggtgca gatcccgcgc ctcaccgccg ccgagcacca gcaccggccc
     1981 cggcagttgt cgcgccgcgt cgaggttggt ggggtcggtc aggatctcgg tcactccacc
     2041 gagccggacc gacgcggcca agtcggcatc ggcccgcatc accacggcga cggcgcccag
     2101 ccgcgacagc gcggcgatgg cgaccagtgc gctgggacgg gtttccatca gtacacccac
     2161 ccggtcgccc tgccgcaccc cgacgtcgat caggccacga acgacgttgt tgacacgtcg
     2221 gttgacggcc tcgtaggtgt gcacgcgccc gtcgaacaac aggaattcgc catgcggggc
     2281 ctcgcgtgcc tgctcttcga tgatgcggcc caacgagata cgggtgtggt cgttgagctg
     2341 tcccagccgg accagtcgtg gcagcgcccg caccgtctcc acggcaagcg tgcgcatcga
     2401 tttgttggcg gcgacaacgg tgttggccgt gccgcgcgcg atgtccagtg cgacttcgga
     2461 gacctctccg acgccgtgcg caagccggga gctgaacgcg acgccgctgt cggtatgttc
     2521 gaccggctga tcggccatca ggtcgatgct ggccggcttg tcgccgccgg tggagagcca
     2581 gcgcacccat tcggcaaccg tgggccaact ctccttggcc gccctcgatc ccacaaccag
     2641 gccgaaatgt cctgtgcgga tcaggtattc gaatacctcg gcgttcggcg ccgcccgccg
     2701 tatcccacgc acggacgcgg gctggccgat gtcgtcgacc tcgccgacga acgccagtat
     2761 cgggcaggtg atatcggtga gcgtcaccat ctgaccgttg atggcgaaac cgccggtcat
     2821 cattcggttg tgcgcgatga actgcttgag cagttcggag atcgccgggc ccgaccatgc
     2881 gatccagcct tcggattcca ggaatctgcg ctgttgttcg cgcggcaaca atgcctcgcg
     2941 atcgtgcagc tgacgcacga aatcgacccg ggccttggcg gttttcaagg ggtccatcat
     3001 ctgaaaaccc atgcgcgcca accagcttgg gatggccaat cggttgaaga cgtgatcggc
     3061 catgaaatcg gcgatcgggg cggcgtagtt cggcggaatg cccatgggca acgcggccag
     3121 ggtgtccacc ggggaaccga acgcgacgat gctggcgatg ttcttcgagc gccggtatgc
     3181 cgcggcctgg taacagaaca tgccgccctg tgagtaaccg accaggtgga cgtcgttgcc
     3241 ggtttcgctc ttcactgtgt cgacggcctg actcagcgcg acgatgtggt cggccagggt
     3301 ccggcgcatg ccgccctcga ccttgtcggg cgagccgaag tcgatgaccc aggggtccag
     3361 cccattggcg tgcaggatcc cgaccgcccc gtcttcccgc gtgacgtccc acatgtccgc
     3421 cgacatcatc atggggtgca ccatcagcac gggcgggcca accggcggct gcccgggccg
     3481 gctgtccggc gggaaatacc gccgaagttt gtacatcggc acactctcga cgatctggga
     3541 cggcgacgga acgctgccgg tttccaggcc cccgagtcgc aagacctcca gtccgttctg
     3601 tgccgtagcg atcagacgct cgaccggtcg cgtgaccatc gaaaaattga gatccaccgc
     3661 tgctccctga cctgcacatc gtgacggctt ggccatcatg gcacatccgc ggttaggcgg
     3721 ttctgatcag cccgtttgcc ggacgctaag gccggccggt ctgccgcccg gcggcgcgtt
     3781 tacgccgtcc ggatcgccgg tacagctgtc cctgcgccgg ctgtggtcgg aattttcgta
     3841 ggaaagttca cgagaggcta gccaaaacag ctacaaggga tcatacttcc gtttattaat
     3901 aaccggtggt ctctgacagc gcagggggca gt
//
LOCUS       DASSGQ010000299        20650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1450180,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000299 DASSGQ010000000
VERSION     DASSGQ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 20650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20650
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450180"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VFI55_13190"
     CDS             <1..546
                     /locus_tag="VFI55_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HET7075021.1"
                     /translation="LAALRSNPDGDCAEVVVAARDLRPGAALTLDDVHLEKRLAATVP
                     DGSQEDLGAVVGSTLAGPTRRGEVLTDVRLLGTRLAESTAGPGARIVPLHLADSALID
                     LVRVGDVVDVLAAPATEPQPTGQPLSRVVATDAVVVLVSARQKAQAADSDRVVLVALP
                     ARVANTVAGSALGQAVTLTLH"
     gene            612..1070
                     /gene="mscL"
                     /locus_tag="VFI55_13195"
     CDS             612..1070
                     /gene="mscL"
                     /locus_tag="VFI55_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET7075022.1"
                     /translation="MLKGFKEFLSRGNIVDLSVAVVIGTAFTALVTKFTDSIITPLIN
                     RIGVNQKSDVSALKIDIGGGQTIDLNVVLSAAINFFLVATVVYFLVVLPYNTLRRKGE
                     VEQADDAEVVLLTEIRNLLAQSNGDSSGKHGGTATAPTPSYGPRADAESQ"
     gene            complement(1096..1653)
                     /locus_tag="VFI55_13200"
     CDS             complement(1096..1653)
                     /locus_tag="VFI55_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HET7075023.1"
                     /translation="MRVDEPSVAGLSELGYTVAAMETGAELVVGRALVVVVDDRTAHG
                     DEDHSGPLVTELLTEAGFVVDGVVAVAADEVEIRNALNTAVIGGVDLVVSVGGTGVTP
                     RDVTPEATRDILDREILGIAEAIRGSGLSAGIVDAGLSRGLAGVSGSTLVVNLAGSRY
                     AVRDGMATLNPLAAHIIGQLSSLEI"
     gene            complement(1653..3196)
                     /locus_tag="VFI55_13205"
                     /pseudo
     CDS             complement(1653..3196)
                     /locus_tag="VFI55_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011742226.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
     gene            complement(3278..4834)
                     /locus_tag="VFI55_13210"
     CDS             complement(3278..4834)
                     /locus_tag="VFI55_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7075024.1"
                     /translation="MMRFRRRRRAPLRATSSLSLRWRVMLLAMSMVAMVVVLMAFAVY
                     AVVSAALYSDIDNQLQSRAQLLIASGSLAADPGKAIEGTAYSDVNAMLVNPGHSIYSA
                     NQPGQTLPVGTTEKAVIRGELFMSRRTASDQRILAIHLPNGSSLLISKSLKPTEAVMT
                     KLRWVLLIVGGIGVAVAAVAGGMVTRAGLRPVARLTEAAERVARTDDLRPIPVFGSDE
                     LARLTEAFNLMLRALAESRERQARLVTDAGHELRTPLTSLRTNVELLMASMEPGAPRL
                     PEQEMVDLRADVLAQIEELSTLVGDLVDLTRDDAGQVVHEPVDMSELIERSLERVRRR
                     RNDIHFDVEVSPWQVYGDAAGLSRAVLNLMDNAAKWSPPGGHVGIRMRQLDPSHAELL
                     VSDQGPGIPPHERRLVFERFYRSTTARALPGSGLGLAIVKKVVLNHGGLLRVEDTVPG
                     GQPPGASIYVLFPGRPMPASTYPTGAINSKMSNTGAETKPVVPVAGNGANSRDSANVI
                     SVDSQSAKAR"
     gene            complement(4831..5517)
                     /locus_tag="VFI55_13215"
     CDS             complement(4831..5517)
                     /locus_tag="VFI55_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7075025.1"
                     /translation="MRILVVDDDRAVRESLRRSLSFNGYSVELAHDGLEALDMIAGNR
                     PDALVLDVMMPRLDGLEVCRHLRSTGDDLPILVLTARDSVSERVAGLDAGADDYLPKP
                     FALEELLARVRALLRRTKPDDAADSVAMTFSDLTLDPVTREVTRGQRRISLTRTEFSL
                     LEMLIANPRRVLTRSRILEEVWGFDFPTSGNALEVYVGYLRRKTEADGELRLIHTVRG
                     VGYVLRETPP"
     gene            complement(5662..5835)
                     /gene="rpmF"
                     /locus_tag="VFI55_13220"
     CDS             complement(5662..5835)
                     /gene="rpmF"
                     /locus_tag="VFI55_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET7075026.1"
                     /translation="MAVPKRRMSRANTRSRRAQWKATPTELVGVTVAGHKHKVPRRLL
                     KAARLGLIDLERR"
     gene            6112..7869
                     /locus_tag="VFI55_13225"
     CDS             6112..7869
                     /locus_tag="VFI55_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HET7075027.1"
                     /translation="MASASGASGASGASGASGAVRIANCSGFYGDRLSAMREMLTGGL
                     DDPVDFLTGDYLAELTMLILGRDRMKHPDRGYAKTFLTQLEECLGEACDRGVRIVANA
                     GGLNPAGLADAVRALAERLGVPVRVAYVEGDDLAPRAAELGLGTPLTANAYLGAWGIV
                     DCLTNGADVVVTGRVTDASVIVGAAAAHFGWGRTDYKELAGAVVAGHVIECGVQATGG
                     NYSFFTEIPAVPGLLDAGFPLAEVYADGSSVITKHPGTGGLVSVDTVTAQLLYEITGA
                     RYANPDATARMDTIQLSADGPDRVRISDVRGEPPPPTYKVSLNSIGGFRNAMTFVLTG
                     LDIEAKAQLVRRQLEAALTVKPAELQWSLARTDHTDANTEETASALLHCVVRDPDPAN
                     VGRQFSSAAVELALTSYPGFHVTAPPGEGQVYGVFTAGYVDAKQVPHVAVHADGTRTD
                     IRCATDTLELTTADCPQLPEPLPVGPTRRVPLGRIAGARSGDKGGSANVGVWVRTEDQ
                     WRWLANALTVELLKVLLPEAADFEVTRHVLPNLRAVNFVIEGILGKGVAYHARFDPQA
                     KGLGEWLRSRYVDIPEGLL"
     gene            7904..9052
                     /locus_tag="VFI55_13230"
     CDS             7904..9052
                     /locus_tag="VFI55_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7075028.1"
                     /translation="MSIWTTPERHQLRKTVRSFTEREILPNVDEWERTGDLPRDLHRR
                     AGAAGLLGANFPEAAGGGGGDGVDSVIICEELHQTGAPGGVFASLFTCGISVPHMIAS
                     GDERLIDTFVRPTLAGEKIGALAITEPGGGSDVGHLRTSAVLDGDHYVVNGAKTYITS
                     GVRADYVVTAVRTGGPGAAGVSLLVIEKGTPGFEVSRKLDKMGWRSSDTAELSFADAR
                     VPAANLVGAQNSGFARIAQAFVSERIALATQAYSSAQRCLDITVQWCRDRETFGRPLI
                     SRQSVQNTLAEMARRIDVARVYSRNVVERQVAGETNLIAPVCFAKNTAVEAGEWVANQ
                     AVQLFGGLGYMAESEVERQYRDMRILGIGGGTTEILIALAAKTLGYQS"
     gene            9132..10727
                     /locus_tag="VFI55_13235"
     CDS             9132..10727
                     /locus_tag="VFI55_13235"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017351375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HET7075029.1"
                     /translation="MTVLQSTLDPKADAYAGAAAAAVSRLDEIDAELAKALAGGGTKY
                     VERHHARGKLTPRERIELLVDPDSPFLELSPLAAYGSAFTVGASIVTGIGAVSGVECM
                     IVANDPTVKGGTSNPWTLKKILRANQIAVENRLPVVSLVESGGADLPTQKEIFIPGGQ
                     MFRDLTRLSAAGIPTIALVFGNSTAGGAYVPGMSDHVVMIKERSKVFLAGPPLVKMAT
                     GEESDDESLGGAEMHARISGLADYFALDELDAIRIGRRIVTRLNWVKQGPKPKSVTEP
                     LFDTEELIGIVPADLRIPFDPREVIARIVDGSDFDEFKPLYGSSLVTGWAQLHGYPVG
                     ILANARGVLFSEESQKATQFIQLANRSDTPLLFLHNTTGYMVGKDYEEGGMIKHGSMM
                     INAVSNSTVPHISVLIGASYGAGHYGMCGRAYDPRFLFAWPSAKSAVMGGAQLSGVLS
                     IVARAAAQARGQQVDDAADASMRAAVEGQIEAESLPLVLSGMLYDDGVIDPRDTRSVL
                     GMCLSAIANGPIKGTSNFGVFRM"
     gene            10813..12819
                     /locus_tag="VFI55_13240"
     CDS             10813..12819
                     /locus_tag="VFI55_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET7075030.1"
                     /translation="MTISRVLVANRGEIARRVFATCRRLGLGTVAVYTDPDAGAPHVA
                     EADARVRLPKTNDYLNAQALIAAARAAGADAVHPGYGFLSENSDFAAAVQAAGLTWIG
                     PPVDAVRSMGSKIESKKLMAAAGVPVLDELDPDTVTQAQLPVLVKASAGGGGRGMRVV
                     RELSLLPAEVAAARREAQSAFGDPTVFCERYLPTGHHVEVQVLADTHGTVWAVGEREC
                     SIQRRHQKIIEEAPSPLVERTPGMRAKLFDAARLAAAAIGYAGAGTVEFLADDDGEFY
                     FLEMNTRLQVEHPVTEETTGLDLVELQLIVAEGGRLDAAPPVGQGHSIEARLYAEDPA
                     HGWQPQAGVVHTFEVPSVRAEFSSLGQRTGIRLDSGLDPKSAGGFTVSIHYDPMLAKV
                     ISYAPTRRQSALVLADALARARLHGLRTNRDLLVTVLRHPAFLDGATDTAFFDTHGLA
                     ELSAPLADDAVVRLSAIAAALADAAHNRALATVFGSIPSGWRNVPSGNQVKGYLDDAG
                     DEQRIEYRFARGGLALPDDESVRLVSATADEVVLSDGGVDCSFAVTRYGPPDNQDVYV
                     DSPRGPVHLTAQPRFPEPGSAVEKGSLIAPMPGNVVRLGAQVGDAVTAGQPLIWLEAM
                     KMEHTVAAPTDGMVVELNVNTGQQVEVGAVLARVEPPQAEGDPQ"
     gene            12816..13991
                     /locus_tag="VFI55_13245"
     CDS             12816..13991
                     /locus_tag="VFI55_13245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7075031.1"
                     /translation="MTDTSFIESDERQALRKAVSEWASSYGAEYYLKKARAHEHTTEL
                     WSEAGKLGFLGVNLPEQYGGGGAGMYELSLVMEEMAAAGSALLLMVVSPAINGTIISR
                     FGTEEQKKRWIPAIADGTLTMAFAITEPDAGSNSHKIITSARRDGSDWILKGQKVFIS
                     GIDQAQAVLVVAHTYRDTGAKTGKLRPALFVVPTDAPGFTYTPIEMELISPERQFQVF
                     LDDVRLPDDALVGAEDAAIAQLFAGLNPERIMGAASSIGMGRFALERAVDYVKTRKVW
                     STPIGAHQGLAHPLAQCHIEVELAKLMMQKAATLYDSGDDMGAAEAANMAKYAAAEAA
                     TRSVDQAVQSLGGNGLTKEYGVAAMLASARLGRIAPISREMVLNFVAQTSLGLPRSY"
     gene            13991..14788
                     /locus_tag="VFI55_13250"
     CDS             13991..14788
                     /locus_tag="VFI55_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HET7075032.1"
                     /translation="MDTLVDYAGPGDAGGPFARLTLNSPHNRNALSTQLVSQLHQGLR
                     DAASDPAVRAVVLGHTGGTFCAGADLSEAGGGDPFDMAAARARELIALLRAIVELPLP
                     VIAAINGHVRAGGFGLVGACDIAVAGPRSTFALTEARIGVAPAIISLTLLPKMSARAA
                     ARYYLTGETFDARQAADIGLITMAAEDVDASVAKVVADVGRGSPQGLAASKALTTAAV
                     LEGFDRNAERLADESARLFVSDEAREGMLAFLQKRPPSWAPVDDAGQ"
     gene            14886..15488
                     /locus_tag="VFI55_13255"
     CDS             14886..15488
                     /locus_tag="VFI55_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HET7075033.1"
                     /translation="MSNSAQRDAEDARDESSRAADTDRIQIAQLLTYAAEQGRLQLKD
                     YEERLTRAYAAKTYEELDQLRADLPGVPINPRRGGKPNPAPSLLLLALLSGFERRGRW
                     NVPKKLTTFTFWGSGVVDLRYADFTSTEVDIHAISIMGVQNILVPPEVNVEIHGHGVM
                     GGFDHSVPGHGTPGAPTVNIHGFSLWGDVGVKRKARIPRG"
     gene            complement(15511..16359)
                     /locus_tag="VFI55_13260"
     CDS             complement(15511..16359)
                     /locus_tag="VFI55_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7075034.1"
                     /translation="MNNVPTIVLNDGVSIPQLGFGVFQIDPDETAAAVRTALDIGYRH
                     IDTAEMYQNEKGVGQGIRDAGLDRADVFITSKLNNGFHKPDDARRAFDKTLAALNSDY
                     VDLFLIHWPLPTLYDGDFVSTWKVLEEFAGDGRARSIGVSNFQPAHLERLADETGTIP
                     AVNQIEVHPYFTNEQVRSYGRDHGIATEAWSPIAQGTVLGDPVITRIADACGKSPAQV
                     VLRWHVQRDDIVFPKSVTPERIKSNFELFDFDLDNSEMDAISALDKGEAGRTGPHPDT
                     FDYVPD"
     gene            complement(16571..18565)
                     /locus_tag="VFI55_13265"
     CDS             complement(16571..18565)
                     /locus_tag="VFI55_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7075035.1"
                     /translation="MAERIKGHKSRYSAYTGGPDPLAPPVDLREALEQIGQDVMGGTS
                     PRRALSELLRRGTKNMPGADRLAAEANRRRRELLRNNNLDGTLQEIKKLLDEAVLAER
                     KELARALDDDARFGELQLDALPPSPAKAVQELSDYQWRSAEARQKYEQIKDLLGREML
                     DQRFAGMKQALEGATDEDRQRVNDMLNDLNELLDKHARGEDTEQDFQDFMDKHGEFFP
                     ENPRNVAELLDSLAKRAAAAQRFRNSLSPDQRAELDALAQQAFGSPALMEALNRLDAH
                     LQAARPGEDWSGSEQFSGDNPFGMGEGTQALSDIAELEQLAEQLSQSYPGATMDDVDL
                     DALARQLGDQAAIDARTLAELERALVNQGFLDRGSDGQWRLSPQAMRRLGETALRDVA
                     QQLSGRRGERDHRRAGAAGELTGATRPWQFGDTEPWNISRTLTNAVLRQAGTATPEGG
                     PSPSIRITVDDVEISETETRTQAAVALLVDTSFSMVMENRWLPMKQTALALNHLVCTR
                     FRSDALQIIAFGRYARTVTAAELTGLEGVYEQGTNLHHALALAGRHLRRHPNAQPVVL
                     VVTDGEPTAHLEDFDGEGTSVFFDYPPHPRTIAHTVRGFDDMARLGAQVTIFRLGSDP
                     GLARFIDQVARRVEGRVVVPDLDGLGAAVVGDYLRSRRRR"
     gene            complement(18558..19943)
                     /locus_tag="VFI55_13270"
     CDS             complement(18558..19943)
                     /locus_tag="VFI55_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7075036.1"
                     /translation="MVTSSSNLPRTVGELRATGHRERGVKQEIRENLLTALAEGDEVW
                     PGILGFDDTVLPQLERALIAGHDVVLLGERGQGKTRLLRALVGLLDEWTPVIGGAELG
                     EHPYRPITPESIRRAATLGDDLPVAWKHRSERYTEKLATPDTSVADLVGDIDPIKVAE
                     GRSLGDPETIAYGLIPRAHRGIVAVNELPDLAERIQVSMLNVMEERDIQVRGYTLRLP
                     LDVLVVASANPEDYTNRGRIITPLKDRFGAEIRTHYPLELEAEMGVIAQEAHLSAQVP
                     DYLLQVLARFARYLRESSSVDQRSGVSARFAIAAAETVAAAARHRGAVLGETDPVARV
                     VDLGTVIDVLRGKLEFESGEEGREQAVLEHLLRRATADTAARVLGGIDVGSLVAAVER
                     GSAVTTGERVSAKDVLAAVPGLPIVDRIAVKLNAESEGERAAALELALEALYLAKRID
                     KVSGEGQTVYG"
     gene            complement(20098..>20650)
                     /locus_tag="VFI55_13275"
     CDS             complement(20098..>20650)
                     /locus_tag="VFI55_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075037.1"
                     /translation="EMREITKRTVAFSAAVAGFALIGSGCHATKSTEATNSATTSAMS
                     SSVTSSTAATSAAPTTTITGANGTPYTVEGPILAKYESLTADQLKDLGKPYDNELKTK
                     DGTGVYQQFDGGVLIYKTGGPVYFVWGKIRDAWNAVQASQGKLGYPTSDEQILPDGSF
                     KSTFEHGTITWKTGDPEATVTMS"
BASE COUNT         3418 a         6964 c         6772 g         3496 t
ORIGIN      
        1 cttgcggccc tgaggtcgaa tcccgacggt gattgcgccg aggtggtggt ggccgcgcgt
       61 gacctccggc ccggtgccgc gttgaccctc gatgatgtac acctcgaaaa gcgtttggct
      121 gcaacggttc ccgacggatc tcaagaagac ctgggcgccg ttgtcggttc caccctggcc
      181 ggcccgacca ggcgcggcga ggtgctcacc gatgtccggt tgctcggtac ccggctggcg
      241 gagtcgaccg cggggccggg cgcccgcatc gtgccgctgc atctggcgga cagcgctctg
      301 atcgacctgg tgcgcgtcgg cgacgtcgtt gacgtgctgg ccgcgccggc caccgagccg
      361 cagccgacgg gccaaccact cagcagggtc gtcgccaccg acgccgttgt ggtcctcgtg
      421 tcggcacggc aaaaagccca ggccgccgat agcgaccgcg tagtgttggt tgcgctgccg
      481 gctcgcgtgg cgaacacggt tgcaggctcg gcgctaggtc aggcggtgac cctcaccctg
      541 cactgagtac ctcgccgctg tcacgcacgt gatcgatagc aaactctgtc caatccagaa
      601 aggacatccg catgctcaag gggttcaagg agtttctctc gcggggcaat atcgtcgacc
      661 tgtccgtcgc ggtggtcatc ggtacggcct tcacggcgtt ggtcaccaaa ttcaccgaca
      721 gcatcatcac gccgttgatc aatcgcatcg gcgtcaacca gaagtccgac gtctcagcct
      781 tgaaaatcga catcggcggg ggccagacca ttgacctgaa cgttgtgctg tcggccgcga
      841 tcaacttctt ccttgtcgcc accgtggtgt acttcctggt cgtgttgccc tacaacacgc
      901 tgcgcaggaa aggtgaagtc gagcaggcgg acgacgcgga agttgtgctg ctcaccgaaa
      961 tccgcaacct tctggcgcaa tccaacggag actcgtccgg caagcacggc gggacggcca
     1021 ccgcaccaac cccgagctac ggaccccgcg cggacgccga atcgcaatga cccgtcgtcg
     1081 cgaattgtcg gacgcctaga tttccaggct cgacaactgc ccgatgatat gggccgcaag
     1141 cggattcagc gtcgccatcc cgtcgcgcac cgcgtagcga gacccagcga ggttgaccac
     1201 caaggtgctt ccggacacgc cggccagacc gcgggacaac ccggcatcga cgatccccgc
     1261 ggacagcccc gacccgcgga tggcttccgc gataccaagg atttcgcgat ccaggatgtc
     1321 gcgggtggcc tccggggtga cgtcacgtgg ggtgactccc gtgccgccga ccgagaccac
     1381 caaatccacc ccaccgatta ccgcggtgtt cagcgcgttg cggatctcga cctcgtcggc
     1441 cgcgacggcc accacaccgt cgacaacgaa tcccgcctcg gtgagcagct ccgtgaccag
     1501 cggcccgctg tgatcctcgt ccccgtgtgc ggtgcgatcg tcgacgacca cgaccagagc
     1561 ccggccgact accaactccg ctcccgtttc catggcagca acggtatatc cgagctccga
     1621 caatcccgcc accgagggct catcgactct catcactgat ccgccttgcc gagggtgacc
     1681 tgcacggtgc ggctcccacc gctgggatcc tggtaggtca gcgacacctt gtcgccgggc
     1741 gccttggacc gcacggcggc caccagcgcg tcggcgctgt tgatcgggcg gtcgtcgacc
     1801 ttggtgacga cgacgccttt gggcactccg gccgccgcgg ccgcaccgtt gggcacgacg
     1861 tcgacaacct tggcgcccgg agcacccttg tcactggtca cctgcacgcc gagcgaggca
     1921 tgcgtcgcct tgccggtagc gatcagctcg tcggcgatgc gcttggcctg gtcgaccgga
     1981 atcgcgaagc ccaggccgat cgacccgctc tgggaatcgg gcgaatcggc gcccagggtg
     2041 gcgatcgccg agttgacgcc caccagctgg ccactcatgt tgaccaacgc gccaccagag
     2101 ttaccggggt tgatcgcggc atcggtctga atcgcgtcca gcacggtgtt ttggttacct
     2161 gactcgccgg tcgtggacac cggccggttc agtgcgctga tgattccggt ggtcaccgtg
     2221 cccgacaacc ccagcggcga cccgatcgcc accaccggct ggccgacccg tagatcggtc
     2281 gaggagccca tcgagacagg ggtgaggccg gacacgccct gcacccgaat cacggcgatg
     2341 tcgctggtcg ggtcggcgcc gaccacggtg aacggtgcgg tacggccgtc tgagaaggtc
     2401 accgtcgttt taggtgctcc cccaccgggg ccacccggac caccgggacc accggggcgc
     2461 cggggcctcc aggtccaccg ggaccgcccg gaccggcggg gccggccggc gcagtgggac
     2521 cgccgcccgg cttggccgcc gcgaccacgt ggttgttggt cagaatcagg ccgtcggccg
     2581 acagcacgat gccggagccc tcttccgact gacggcccag atcggtctcc aacatcacga
     2641 cgctgggcac caccttggcc gcgacctgtt cgacgctgcc cggcggcatg ttcgcggccg
     2701 ggacgctggg agcgaccccc ggaattatcc cgtgaccatt gccgcccgcg gaatgcgtac
     2761 ccagctcaac ggctgttgcc gcggcgccgc cgatgccggc cgacaccacc gcgatcgcca
     2821 acgcgctcac agccaacaca cctgcgcggg aacgcttttg gggcatgggc gcaggcggga
     2881 aaggcatcgg cggcagcatc ccgggcatcg gacccgcccc ggtacccccg ggaatcggac
     2941 ccgggccggt gccaccgaca ccgggggccg gccccgcacc cggagggaca gggcccggcc
     3001 cggtgccggc gaacggctcg taaggccgcc ggtacggggc cgcctgcgtc ggctgcgacg
     3061 gctgataacg ccagtcgaac tgctggttgt agggcgactg gtgcccctgg acataggctg
     3121 ggggcacagg ctgattaggc ccggtacggt atcccggctg ctgcggctgc ggcgaatacc
     3181 tcgggtcatt cgtcatgtcg ctgggtcgct cttcctctaa tcggacattg ggctcatgtt
     3241 ggctcggctt ggcttgattc caacagtaac tgcagcacta ccttgccttc gcggactgag
     3301 agtccactga gataacgttc gccgaatccc gagagttcgc cccgttgccc gcaacgggca
     3361 ccaccggctt ggtctcggct cctgtattgc tcatcttact gttgatcgcc cctgtcgggt
     3421 acgtcgaagc cggcatcggc cgcccgggaa acagcacgta gatggatgcg cccgggggct
     3481 gcccgcctgg cacggtgtcc tctacgcgca gcaacccgcc gtgattgagg accactttct
     3541 tgacgatcgc cagtcctagc cccgaccctg gcagcgcccg cgccgtcgtc gaacggtaga
     3601 agcgctcgaa caccagacgc cgctcgtggg gtggaatgcc cggaccctgg tcggagacca
     3661 acagctcggc gtgcgatgga tcgagttgcc gcatcctgat gccgacatga ccgcctggcg
     3721 ggctccactt ggccgcgttg tccatgaggt tcagcaccgc gcgcgacagc ccggcggcgt
     3781 cgccgtagac ctgccatggg cttacctcga catcgaagtg gatgtcgttg cgccgccgcc
     3841 tgactcgctc caggctgcgc tcgatcagct cggacatgtc gaccggttcg tgcaccacct
     3901 ggccggcatc gtcgcgggtg aggtcaacca aatcgcctac cagagtggac aattcctcga
     3961 tctgcgcgag cacatcggca cgcaggtcca ccatctcctg ctcgggtagc cgcggcgccc
     4021 ccggttccat cgaggccatc agcaattcga cgttggtgcg cagcgacgtc agcggggtgc
     4081 gcaattcatg tccggcatcg gtgaccagcc gtgcctgccg ttcccgggac tcggccagcg
     4141 cccgcagcat caaattgaat gcctctgtga gcctggccaa ttcgtcactg ccgaataccg
     4201 ggatgggccg caggtcgtcg gtgcgcgcca cccgttcggc ggcttcggtc aggcgggcca
     4261 ccgggcgcag ccccgcccgg gtgaccatgc cccccgccac cgcggcgacc gcgacaccaa
     4321 tcccgccgac gatgagcagc acccagcgca gtttggtcat gaccgcttca gttggcttca
     4381 ggctcttgga gatcaacagc gaactgccgt tgggcagatg gattgcaagg attcgttgat
     4441 ccgaagccgt acgacgcgac atgaataatt cgccgcggat gacagccttc tccgtggtgc
     4501 cgacgggcag ggtctgaccg ggctggttgg cggagtagat cgagtggccc gggttcacca
     4561 gcatcgcgtt gacgtccgaa taggcggtgc cctcgatggc tttgcccggg tcggcggcca
     4621 gcgagccgct ggcgatcagc agctgcgccc ggctctgcag ctggttgtcg atatcgctgt
     4681 acagcgcggc cgaaaccacc gcgtagacgg cgaatgccat caatacgacg accatcgcca
     4741 ccatcgacat cgccaacagc atcacccgcc accgtaggga caaggagctg gtggctcgca
     4801 gcggtgcgcg ccgccgccga cggaatctca tcagggcggc gtctcccgaa ggacataccc
     4861 cactccgcgc accgtatgga tcagtcgtag ctcaccatcg gcctcggttt tgcgccgcag
     4921 atagccgacg tagacctcca gcgcattgcc cgaggtggga aagtcgaatc cccacacctc
     4981 ctcgaggatg cggctgcggg tcagcacccg gcgcgggttg gcgatcaaca tctccagcag
     5041 ggaaaactcg gtgcgggtca agctgatgcg acgttgcccg cgcgtgactt cgcgggtcac
     5101 cgggtccagg gtcaggtcgg agaacgtcat ggccactgag tcggcggcat cgtctggctt
     5161 ggtgcggcgc agcaacgccc gcacacgcgc caacagctct tcgagcgcga acggtttcgg
     5221 cagatagtcg tcggccccgg cgtccaggcc ggccacccgc tcggacacgg agtcgcgggc
     5281 ggtaagcacc aggatcggca ggtcgtcgcc ggtgctgcgg aggtgacggc acacctcgag
     5341 gccgtccaga cgcggcatca tcacgtccag aacgagcgcg tcgggccggt tgccggcgat
     5401 catgtcgagc gcctcgagcc cgtcgtgggc cagttcaacc gagtagccgt tgaacgaaag
     5461 cgacctgcgt agcgattcgc gcaccgcacg atcgtcgtcg acgacaagta tccgcacggc
     5521 caccagtttc gtcctagcgc ctgagagcgg cctgagaggc gcgccgcata gtcgatctga
     5581 aatccctcga tgacgtcgcg ggcccaaggc cggccctggg ggcacctccg cccgctggcg
     5641 cgggcgcgtc aggcaacctg attagcggcg ttcgagatcg atgaggccga ggcgggccgc
     5701 cttgagtagc cgccgaggca ctttgtgctt gtggcctgcg accgtcacgc cgacgagctc
     5761 ggtcggggtg gccttccact gcgcgcgacg gcttcgggtg ttcgcgcgcg acatcctgcg
     5821 cttgggtacg gccatggtcg ggcctaactc ctcaggtggg gcttcgggtg ggacatgtcg
     5881 cggggcatgg cccataggcc cgcacgtgcg acgatcgcgt accaggatag tcgctggcct
     5941 gctggtcgcc aaaatggcgt ccatcgcgag gctcatccac cttcgccgga catgccggac
     6001 cggcatcgac atcgaccggg catccttgac ttgacaccgg cggtacccgc taacctaccg
     6061 ggtggttggt tgattcgcgc tcaccggtaa ttccacgaat ggaggacgcg catggcctct
     6121 gcttctggtg cttctggtgc ttctggtgct tctggtgctt ctggcgctgt ccggatcgcg
     6181 aattgctccg ggttctacgg cgaccggctg tctgcgatgc gcgagatgct caccggcggc
     6241 ctggatgatc cggtggactt cctcaccggc gactacctgg ccgaactcac catgctcatc
     6301 ctgggtcgcg accggatgaa gcaccccgac cgcggctatg ccaagacgtt cctgacccaa
     6361 cttgaagaat gcctgggtga ggcctgcgac cgcggggtcc gcatcgtcgc caacgccggc
     6421 ggcctgaacc ccgccggtct tgccgatgcg gtgcgcgcct tggccgagcg cctgggcgtg
     6481 ccggtgcggg tcgcctacgt cgaaggtgac gacctggcac cgcgtgcggc cgaactgggg
     6541 ctgggcacgc cgctgaccgc aaacgcgtac ctcggtgcgt ggggcatcgt cgactgcctc
     6601 accaacggcg ccgacgtcgt cgtcaccggc cgggtcaccg acgcctcggt gatcgtcggg
     6661 gcggcggcgg cacacttcgg ctgggggcgt accgactaca aggaactcgc cggcgccgtg
     6721 gtcgccggcc acgtcatcga atgcggcgtc caggccaccg gcggcaacta ctccttcttc
     6781 accgaaatcc cggcggttcc cggcctcctc gacgccggtt tccccctggc cgaggtatac
     6841 gccgacggtt cgtcggtgat taccaagcac cccggcaccg gcggtctggt gagcgtcgac
     6901 accgtcaccg cgcagttgct ctacgagatc accggagccc gctacgccaa ccccgacgcc
     6961 accgccagga tggacaccat ccagctgtcc gccgacggcc ccgatcgcgt ccgcatcagc
     7021 gatgtccgcg gcgaaccgcc cccacccacg tacaaggtgt cgctgaacag catcggcggg
     7081 ttccgcaacg ccatgacatt cgtcttgacc ggcctggaca tcgaggccaa agcgcagctg
     7141 gtgcgccggc agctcgaggc cgcgctcacc gtcaaaccag cggagctgca gtggtcattg
     7201 gcccgcaccg accacaccga cgccaacacc gaagagaccg ccagcgcgtt gctgcactgc
     7261 gtcgtccgcg atcccgaccc ggccaacgtc gggcgccaat tctcgtcagc tgcagtggaa
     7321 ttggcgctta ccagctatcc cgggtttcac gtgaccgccc cgccgggcga aggtcaggtc
     7381 tatggcgttt tcaccgcggg ttacgtcgac gccaaacaag taccgcacgt cgcggtgcac
     7441 gccgatggga cccgcaccga cattcgttgc gccactgaca ctttagaact gacgacggcc
     7501 gattgcccgc agttgcccga gccgctcccg gtcggtccga cccgacgggt accgctgggc
     7561 cgcatcgccg gggcccgcag cggcgacaag ggcggatcgg ccaatgtcgg tgtctgggtg
     7621 cgcaccgagg accaatggcg atggctcgcc aacgcgctga ccgtcgagct gctcaaggtg
     7681 ctgctgcccg aggcggcgga cttcgaagtc acccgccacg tgctgcccaa tctgcgcgcg
     7741 gtgaacttcg tcatcgaggg catcctgggt aagggcgttg cctatcacgc acgcttcgat
     7801 ccccaggcca aggggctggg cgaatggttg cgcagccgat acgtcgacat tccggagggg
     7861 ctgttatgaa tcgttggacg actatgaatc tttggacgac ccggtgagta tctggaccac
     7921 tccggagcgc caccagctgc gaaagaccgt gcgctcgttc acggaacgcg agatcctgcc
     7981 caacgtcgac gagtgggaac gcaccggcga cctgccccgt gacttgcacc ggcgcgccgg
     8041 tgccgcgggg ctgctgggcg cgaacttccc cgaagcggcc ggcggtggcg gcggggacgg
     8101 cgtcgattcg gtgatcatct gcgaggaact gcatcagacc ggcgcacccg ggggcgtttt
     8161 cgcgtcgttg ttcacctgcg gcatctcagt gccgcacatg atcgcgtcgg gcgatgagcg
     8221 gctgatcgac accttcgtcc ggccgacgtt ggcaggcgag aagatcggcg cactcgccat
     8281 caccgagcca ggcggcggtt cggacgtcgg gcacctgcga acctctgcgg tgttggatgg
     8341 cgatcactac gtcgtgaacg gcgccaagac ctacatcacc tccggagtcc gcgccgatta
     8401 cgtcgttacc gcggtccgca ccggaggtcc cggcgctgca ggggtttcgc tgctggtgat
     8461 cgagaagggt acgcccggct tcgaggtgag ccgcaagctg gacaagatgg ggtggcggtc
     8521 ttccgatacc gcggagctgt ccttcgccga cgcgcgcgtg cccgcggcca atctcgtcgg
     8581 cgcccagaac agcggattcg cccggatcgc ccaggccttc gtctccgagc gcatcgcgct
     8641 tgccacgcag gcatattcga gcgcgcagcg atgtctggac atcaccgtgc aatggtgtcg
     8701 cgatcgagag acgttcggcc gcccgctgat atcccgccag tcggtacaga acacgttggc
     8761 agagatggcc cgccgtatcg acgtcgcgcg ggtctattcg cgcaacgtgg tggagcgcca
     8821 ggtggcggga gagaccaacc tgatcgcgcc ggtgtgcttc gccaagaaca ccgccgtcga
     8881 agccggggaa tgggttgcca accaggccgt ccagttgttc ggcggtctgg gctacatggc
     8941 cgaatccgaa gtcgaacgcc agtaccggga tatgcggatc ctgggtatcg gcggcggaac
     9001 caccgaaatc ctcatcgcgc tggccgccaa aacccttggg taccaatcat gatgacaccg
     9061 ccactccccc gcacactggt gggggtaccg ccctgcgggg acgtaccccg actgcatcgt
     9121 cggcggtcgt catgaccgtc ttgcagtcca cgctcgaccc aaaggctgat gcctatgccg
     9181 gcgcagctgc ggcggcagtg tcaaggctcg acgagatcga cgccgaactc gccaaagcgc
     9241 ttgcgggagg gggaaccaag tacgttgaac gtcaccacgc ccgcggcaag ctgacacccc
     9301 gggaacgtat cgagctgctt gtcgaccccg actccccgtt cctggaactg agtccgctgg
     9361 cggcttacgg cagcgcgttc acggtcggcg ccagcatcgt caccggcatc ggcgcggtga
     9421 gcggcgtgga gtgcatgatc gtcgccaacg accccaccgt caaaggtggc accagcaacc
     9481 cgtggacact gaaaaagata ctgcgggcca accagatcgc cgtggagaac cggttacccg
     9541 tggtttcgct ggtggaatcc ggcggcgcgg acctgcccac ccagaaggag atcttcatcc
     9601 ccggcgggca gatgttccgc gacctgaccc ggctgtctgc ggccggcatc ccgaccatcg
     9661 ccctggtctt cggcaactcc accgcgggcg gggcctacgt ccccgggatg tccgaccacg
     9721 tggtgatgat caaggaacgt tcgaaggtct ttctggccgg tccgccgctg gtgaagatgg
     9781 ccaccggcga ggaatccgac gacgagtctc tcggtggcgc cgaaatgcac gcccgcatct
     9841 cgggtttggc cgactacttc gcgctcgacg aactcgacgc gatccggatc ggacggcgca
     9901 tcgtgacgcg gctgaactgg gtcaagcaag ggcccaagcc gaaatccgtg acagagccgc
     9961 tgttcgatac cgaggaactg atcggcatcg tgcccgcgga cctgcgcatc ccgttcgatc
    10021 ctcgtgaggt gatcgcccgc atcgtcgacg gctcggactt cgacgagttc aagccgctgt
    10081 acggctcgtc gctggtgacc ggatgggcac agctccacgg ctacccggtg ggtatcctgg
    10141 ccaacgcgcg gggtgtgttg ttcagcgagg agtcgcagaa ggccacccag ttcatccagc
    10201 tggccaaccg ctccgacacg ccactgttgt tcttgcacaa caccaccgga tacatggtgg
    10261 gcaaggacta cgaggagggc ggcatgatca agcacggttc gatgatgatc aacgccgtgt
    10321 ccaactcgac ggtcccgcac atctcggtgc tgatcggcgc ctcctacggc gccgggcatt
    10381 acggcatgtg cgggcgtgcc tacgatcccc ggttcctctt cgcgtggccg agcgcgaagt
    10441 ccgcggtgat gggcggtgcc cagctgtcgg gcgtgctgtc gatcgttgcc cgggcggccg
    10501 cccaagcccg cgggcagcag gtggacgacg ccgccgacgc ttccatgcgg gcggccgtcg
    10561 agggccaaat cgaagccgag tcgctgcccc tggtgctatc cgggatgctc tacgacgacg
    10621 gggtgatcga cccgcgcgac acccgcagcg tgctgggaat gtgtttgtcc gccatcgcca
    10681 atggcccgat caaagggacg tcgaacttcg gcgtcttcag gatgtgatag cgatgtgggg
    10741 gcactccccc agagggcggt acccccaccc gcttgcgggg gacgcttgcg cgaggagccg
    10801 aaggtggagc tcatgacaat ttcccgagtg ttggtggcca accgcggtga gatcgcccga
    10861 cgggtgttcg ccacctgccg gcgactgggc ctgggcaccg tcgccgtcta caccgacccc
    10921 gacgccggcg caccgcacgt cgccgaggcc gacgcccggg tgcggctgcc gaagaccaac
    10981 gactatctga acgcccaggc cctcatcgcg gccgcgcgcg ccgccggcgc cgacgccgtc
    11041 caccccggct acggattcct ttcggagaat tcagatttcg cggccgcggt gcaggccgcg
    11101 ggcctgacct ggatcggtcc gccggtggac gcggtccggt cgatgggttc caagatcgag
    11161 tccaagaagc tcatggccgc cgccggggtg ccggtgctcg acgaactcga tcccgacacg
    11221 gtcacccaag cgcagctgcc ggtgctggtg aaagcctcgg cgggcggcgg cggacgcggc
    11281 atgcgggtag tccgcgaatt gtcactgctg cccgccgaag tcgccgctgc ccggcgcgag
    11341 gcgcagtccg cgttcggcga cccgaccgta ttctgcgagc gttacctgcc caccggacac
    11401 cacgtcgagg ttcaggttct cgccgacact cacggcacgg tgtgggcggt cggtgaacgc
    11461 gaatgctcga ttcagcgtcg gcaccaaaag atcatcgaag aggccccgtc gccgttggtc
    11521 gaacgcacac cgggcatgcg cgccaagctg tttgacgctg cccggctggc cgcggccgcg
    11581 atcggctacg ccggcgccgg tacggtcgag ttcctcgccg atgatgacgg cgagttctac
    11641 ttcctggaga tgaacacccg gctgcaggtc gagcatccgg tcaccgaaga gaccaccggg
    11701 ctcgatctgg tcgagctgca gctcattgtt gccgagggcg gccggctgga tgcggctccg
    11761 cctgttggcc aaggacattc gatcgaggcc cgactctacg ccgaggatcc cgcgcatggt
    11821 tggcaaccac aggccggcgt ggtccacacg ttcgaggtgc cttcggttcg ggccgaattc
    11881 agctcactgg gacaacggac cgggatccgg ttggactccg gcctggatcc caagtccgcc
    11941 ggtggattca cggtgtcgat ccactacgac ccgatgcttg ccaaggtcat ctcgtatgcc
    12001 ccgacgcgtc ggcagtccgc gctggtactg gccgacgcgc tggcgcgtgc ccgcctgcac
    12061 ggcctgcgca ccaaccgcga tctgctggtg accgtgctgc gtcatcctgc cttcctcgac
    12121 ggggccacgg acacagcgtt tttcgacacg cacggccttg ccgagctgtc ggcgccgctg
    12181 gccgacgacg cggtggtccg gttatcggcg atcgcggccg cacttgccga cgccgcgcat
    12241 aaccgcgctc tcgccaccgt cttcggctcg attcccagtg gctggcgaaa cgtgccgtcc
    12301 ggcaaccagg tcaagggcta cctggacgac gccggcgacg agcagcgcat cgaataccgg
    12361 ttcgccagag gcggattggc gcttccggat gacgaatcgg tgcgtctcgt ctcggccacg
    12421 gcggacgagg tggtgctgtc cgacggcggg gtggactgca gcttcgcggt gacacgctat
    12481 ggccctcccg acaatcagga cgtctacgtc gattcgccac gcggccccgt tcacctcacg
    12541 gcgcaacccc ggttccccga gcctggttcg gccgtcgaga aggggtcgct gatagcgccc
    12601 atgcccggca acgtcgtccg tctcggcgcc caggtcggcg acgccgtcac cgccggccag
    12661 ccgctgatct ggctggaggc gatgaagatg gaacacaccg tcgccgcacc gaccgacggc
    12721 atggtcgtcg agctcaacgt caataccggt cagcaagtcg aagtgggcgc cgtcctggca
    12781 agggtggaac caccgcaagc agaaggagat ccacagtgac cgataccagc ttcatcgaga
    12841 gcgacgaacg ccaggcgctg cgcaaggcag tgtccgagtg ggcgtccagc tacggcgctg
    12901 agtactacct gaagaaggcg cgcgcccacg aacacaccac cgaattgtgg tccgaagcag
    12961 gaaaactcgg attcctcgga gtgaatctgc cggagcagta cggtggtggc ggggccggaa
    13021 tgtatgagct gtcactggtg atggaggaga tggcggccgc gggcagcgcc ctgctgctga
    13081 tggtggtgtc gcccgcgatc aacggcacca tcatcagcag gttcggcacc gaagaacaga
    13141 agaagcgctg gattccggcc atcgccgacg gcaccctgac gatggcgttt gccatcaccg
    13201 aacccgacgc gggatccaac tcgcacaaga tcatcaccag cgctcgtcgc gatggcagcg
    13261 actggatcct caagggccag aaggtcttta tctccggcat agaccaggcg caggcggtgc
    13321 tcgtggtggc tcacacgtac agggacaccg gggccaaaac cggcaagctg cgcccggcac
    13381 tgttcgtggt gcccaccgac gcgcccggct tcacctacac cccaatcgaa atggagctga
    13441 tcagccccga gcgccaattc caggttttcc tcgacgatgt gcggctgccg gacgatgcac
    13501 tggtcggagc ggaggatgcc gccatcgcgc agcttttcgc cggcttgaac cccgagcgca
    13561 tcatgggcgc ggcgagctcg atcggcatgg gacgtttcgc gctggaaagg gccgtcgact
    13621 atgtcaagac acgcaaggtg tggtccactc ccatcggtgc tcatcaaggg ttggcacatc
    13681 cgttggcgca gtgtcacatc gaggtggaac tcgcgaagct gatgatgcaa aaggcggcga
    13741 cgctctacga cagcggcgac gacatgggtg cggccgaagc cgccaacatg gccaaatacg
    13801 ccgcggccga agcggccacc cgctcggtcg accaggccgt tcagtcgctg ggcggcaacg
    13861 ggctgaccaa ggagtacggt gttgccgcca tgctcgcctc ggcccggctg ggtcggatcg
    13921 ccccgatcag ccgcgagatg gtgctgaact tcgtggcgca gacatcgctg ggcctgcctc
    13981 gaagctactg atggacacgc tggtcgacta cgccggtccg ggcgatgctg gaggtccgtt
    14041 cgcgcggctg acgctgaact ctccgcataa tcgcaacgcg ctctcgacgc agctcgtgag
    14101 ccagctgcat caggggctgc gtgacgccgc ttcggatccg gcggtgcggg cggtggtgct
    14161 ggggcacacc ggcggcacct tctgcgccgg cgccgatctg agcgaggcgg gcgggggcga
    14221 cccgttcgac atggccgccg cccgggcccg agaactgatc gccctattgc gcgccatcgt
    14281 cgagttgccg ctgccggtga tcgccgccat caacggtcat gttcgggcgg gcgggttcgg
    14341 cctggtcggt gcctgcgata tcgcggtcgc cggcccgcgc agcaccttcg cgctgaccga
    14401 ggcaaggatc ggggtggccc cggcgatcat ctcgctgacg ctgctgccga agatgtcggc
    14461 gcgggcggcg gcgcgctatt acctgaccgg cgagacgttc gatgcccgcc aagcggcgga
    14521 catcggactc atcacgatgg cggccgagga cgtggacgct tcggtggcca aggtggtcgc
    14581 cgacgtgggc cgcggctctc cccagggtct tgcggcgtcg aaggccttga ccacagccgc
    14641 cgtgctcgaa gggttcgacc gcaacgccga acggctcgcc gacgagtcgg cccgcctgtt
    14701 cgtgtccgac gaggctcgtg aaggcatgct ggcgtttctg cagaagcgtc cacccagttg
    14761 ggcgccggtc gacgatgccg gccagtgacg cgaagccgac cgcccggcgg tcaagtccgg
    14821 caaggtaggt tgcagtttcg ccaaccctct tgcagcagga cttgtacgtc gtacgctcgt
    14881 ggataatgag caactcggcg cagcgggatg cggaggacgc gcgtgacgaa tcgtcgcgtg
    14941 cggctgacac ggatcgaatc cagatcgcac agttgctgac atacgcggcc gagcagggcc
    15001 gcctgcagct gaaagactat gaagaacgcc tgaccagggc ttatgcggcg aaaacgtatg
    15061 aggagctgga tcagctcagg gccgacctgc cgggcgtgcc gatcaacccg cgccggggcg
    15121 gcaaacccaa tccggcgccg tccttgttgc tcctggccct gctgagtggt ttcgagcggc
    15181 gtggccgatg gaatgtgccg aagaagctga ccacgttcac cttctggggc agtggggtgg
    15241 tggatctgcg ctacgccgat ttcacttcca cggaggtgga cattcacgcg atctcgatca
    15301 tgggcgtcca gaacattttg gtgccacccg aagtcaacgt cgagatccac ggccacggtg
    15361 tgatgggtgg gttcgaccac agcgtcccgg gccatggcac gcctggcgcg ccgacggtga
    15421 acatccacgg cttctcgctc tggggcgatg tgggcgtcaa gcgcaaagcc cgcatacccc
    15481 gcggctagcg ccggcggcga cctttagttg ctagtcgggc acgtaatcga aggtgtccgg
    15541 gtggggtccg gtcctgcccg cttcaccctt atcaagcgcc gagattgcgt ccatctcgga
    15601 attgtccaaa tcgaaatcga acagctcgaa gttggacttg atccgctccg gcgtcaccga
    15661 tttggggaac acgatgtcgt cgcgctggac gtgccaacgc aacaccacct gcgccggcga
    15721 tttgccgcag gcatcggcga tccgagtgat caccggatcg ccgagcaccg tgccctgggc
    15781 gatcggcgac catgcctcgg tggcaatgcc gtgatcgcgt ccgtaggagc ggacctgctc
    15841 gttggtgaag tagggatgga cctcgatctg gttgaccgcg gggatggtgc cggtttcgtc
    15901 ggcgagccgc tccagatggg cgggctggaa gttcgagacc ccgatgctgc gtgcgcgccc
    15961 gtcacctgcg aactcttcga gaaccttcca ggtggaaacg aaatctccgt cgtagagcgt
    16021 cggcagcggc cagtgaatga ggaacaggtc gacgtaatcg gaattcaggg ctgccaatgt
    16081 cttgtcgaat gcgcgccggg cgtcatcggg cttgtgaaag ccgttgttga gtttgctggt
    16141 aatgaatacg tcggcacggt cgagaccggc gtcacgaata ccttgaccga cgcccttttc
    16201 gttttgatac atctccgcgg tgtcgatgtg gcggtatccg atgtcaagcg cagtccgcac
    16261 cgccgcggcg gtctcgtcgg ggtcgatctg aaacacaccg aaaccaagct gcgggatgct
    16321 tacaccatcg ttgagcacga tcgttggcac gttgttcatg ggtttcctcc ttcttgcacc
    16381 ttgtacccgg tcgaccaaac tcctaccggg tcgcgcgacc acggccctcc caggctgtgg
    16441 acgacctcac caccgtcgtc cacagcggcc gtcctgtcgt cattgctggc acccatcccc
    16501 gtcacccacc agcagcacca cgcgggtacc cgccagctgc aattccgggg cggctgcgta
    16561 cccgcgcccg ctagcgacgg cgccgcgacc gcaggtagtc gccgaccacg gcggctccga
    16621 ggccgtcgag atcggggacc acgacgcgtc cctcgacgcg gcgggcgacc tggtcgatga
    16681 aacgcgccag gccaggatcg ctgcccagcc ggaaaatcgt cacctgcgca ccgagccggg
    16741 ccatatcgtc gaacccgcgc acggtgtggg cgatggtccg cgggtgcggc gggtaatcaa
    16801 agaacaccga cgtgccctca ccgtcgaagt cctccagatg agcggtcggc tcaccgtcgg
    16861 tcaccacgag cacgacgggt tgcgcgttgg ggtgccggcg caggtgacgg cccgccagcg
    16921 ccagcgcgtg atgcaggttg gtgccctgct cgtagacgcc ttccagaccc gtgagctcgg
    16981 cggccgtcac cgtccgggcg tagcggccaa aggcgatgat ctgcaaggca tctgagcgga
    17041 accgtgtgca caccaggtgg ttgagcgcga gcgccgtctg cttcatcggc agccagcgat
    17101 tctccatcac catcgaaaac gaggtgtcga ccagcagcgc caccgccgcc tgcgtgcggg
    17161 tctcggtctc gctgatctcg acgtcgtcga cggtgatgcg aatggacggg ctggggccgc
    17221 cttccggagt cgcagttccc gcctgccgca gcacggcgtt ggtaagcgtg cgggagatgt
    17281 tccacggctc ggtgtcaccg aattgccagg ggcgggtcgc gccggtcagc tccccggccg
    17341 cccccgcccg ccgatggtcg cgctcgccgc gtcggcccga aagctgttgg gccacgtcgc
    17401 gtagcgcggt ctcacccagc ctgcgcatcg cttgggggga aagtcgccac tggccgtcgg
    17461 agccacggtc caggaaaccc tgattgacca gcgcgcgttc caactcggcc agcgttcggg
    17521 catcgattgc ggcctggtca ccgagctggc gcgccaacgc gtcgagatcg acgtcgtcca
    17581 tggttgcgcc cggatagctc tgcgagagct gctcggccag ctgctccagc tcggcgatat
    17641 cggacagtgc ctgggtgccc tcgcccatgc cgaacgggtt gtcgccggag aactgctccg
    17701 atccgctcca gtcctcaccc ggccgcgcgg cctgcagatg cgcatcgagc cgattcaacg
    17761 cctccatcaa agccggcgag ccaaatgcct gctgtgccaa ggcatccagc tcggcccgct
    17821 ggtccgggct caggctgttg cggaagcgct gggcggccgc ggcacgcttg gcgagcgagt
    17881 cgagcagctc agccacgttg cgcgggttct ccgggaagaa ctcgccgtgt ttgtccatga
    17941 agtcttgaaa gtcctgctcg gtgtcctcac cacgggcgtg cttgtccagc agctcgttga
    18001 gatcattcaa catgtcgttg acgcgctggc ggtcctcgtc ggtggcgcct tccagcgctt
    18061 gcttcatgcc cgcgaaacgt tggtccagca tctcgcggcc gagcaaatcc ttgatctgct
    18121 cgtacttttg gcgggcttcc gcgctgcgcc actgatagtc ggacaactcc tgaacggcct
    18181 tggccggcga gggcggcaac gcgtccagct gcagctcgcc gaagcgggcg tcgtcgtcca
    18241 gcgcacgggc cagctccttg cgttcggcca gcaccgcctc gtcgagcagc ttcttgatct
    18301 cctgcagggt cccgtctaag ttgttgttcc gcaacaactc ccgtcggcgc ctattggcct
    18361 cggcggcaag tcgatcggct ccgggcatgt tcttggtgcc gcggcgcaac agttccgaca
    18421 gcgcacggcg cggcgaggtg ccgcccatga cgtcctgtcc gatctgttcg agcgcctcgc
    18481 gcaggtccac cgggggggcc agcgggtcag gccctccggt gtacgccgag tatcgcgact
    18541 tatgcccttt aatacgttca gccataaacg gtttggcctt ccccggacac cttgtcgatc
    18601 cgcttggcca ggtacagcgc ttccagcgcc agttccagcg ccgcggcccg ctcgccttcg
    18661 gactcggcgt tgagcttgac cgcgattctg tccacgatgg gcagccccgg caccgcagcc
    18721 agcacgtcct tggccgacac ccgctcgccc gtcgtgaccg cggaaccgcg ctcgaccgcg
    18781 gccaccagcg aaccgacgtc gatgccgccc aacactctgg cggcggtgtc ggcggtcgcg
    18841 cgacgcagga gatgctccag caccgcctgt tcgcggccct cctctccgga ctcgaattcc
    18901 agcttgccgc gcaggacgtc gatcaccgtg cccagatcga cgacgcgagc caccgggtcc
    18961 gtctccccca gcaccgcgcc gcggtgccgg gccgcggccg caacggtctc ggccgctgcg
    19021 atggcgaacc gggccgatac ccccgaccgt tgatcgaccg aactggactc ccgcaggtaa
    19081 cgagcgaacc gcgccagcac ctgcagcagg tagtcgggca cctgagcgct caggtgtgcc
    19141 tcctgggcga tgacgcccat ctccgcctcc agctccagcg ggtagtgcgt tcggatttcg
    19201 gcgccgaacc ggtccttgag cggagtgatg atgcgacccc ggttggtgta gtcctcgggg
    19261 ttggcgctgg cgaccaccaa cacgtccagc ggcaggcgca gcgtgtagcc gcgtacctgg
    19321 atatcgcgct cctccatgac gttgagcatt gacacctgga tgcgctcggc gagatcaggc
    19381 agttcgttga ccgcgacgat gccgcggtgg gcgcgcggga tcaggccgta ggcgatggtc
    19441 tccgggtcgc ccaggctgcg gccctcggcg accttgatcg ggtcgatgtc gccgaccagg
    19501 tcggcgacgc tggtgtccgg ggtggccaac ttctcggtgt atcgctcgct gcggtgcttc
    19561 caggccaccg gcaggtcgtc gccgagtgtg gcggcccgcc gaatcgactc cggcgtgatc
    19621 ggccggtagg gatgctcgcc gagttcggcg ccgccgatca ccggtgtcca ctcgtccagc
    19681 aacccgacca gcgcccgcag caaccgggtc ttaccctggc cgcgttcgcc aagcagcacg
    19741 acgtcgtgac ccgcgatcag cgcccgctcc agctgaggca aaacggtgtc gtcgaagccc
    19801 aggatgcccg gccagacctc gtctccctcc gccagcgcgg tcagcaggtt ttcgcggatc
    19861 tcctgcttga cgccccgttc acggtgaccg gtggcacgta gttcgccaac ggtgcgaggc
    19921 aggttgctcg atgatgtcac cactccacgc tacgacggtg ccgtggatac ggcaccgcgc
    19981 cctcccccgg gacgaccagg ccgagtcctt cgaagcgaag gtcgggcaat cgcaccgact
    20041 gcccgacctc cggcacatgt ctttcagccc accggaatcg gtggacgcga agaccggcta
    20101 gctcatggtg acagtggcct ccggatcgcc tgttttccag gtaatcgtgc cgtgctcgaa
    20161 cgtcgactta aaggagccgt cggggagaat ttgctcgtca gaggtcggat aacccagctt
    20221 gccttgcgaa gcctgcaccg cattccatgc gtcgcgaatc ttgccccata cgaagtagac
    20281 cgggccgcca gtcttgtaaa tgagcacgcc gccgtcgaac tgctggtaga cgccggtacc
    20341 gtccttggtc tttagttcgt tgtcgtacgg tttgcccagg tccttcagtt gatcggcggt
    20401 aagggactcg tacttcgcga gaatgggtcc ctccacggtg tacggcgtgc cattggcacc
    20461 cgtgatggtt gtcgtcgggg ccgcgctggt tgcggcagtt gaggacgtta cggacgaaga
    20521 catcgcggac gtagtggctg aatttgtcgc ttcggtggat ttggtcgcgt ggcacccgga
    20581 gccgatcaga gcaaaccccg caaccgcagc ggaaaatgcc actgttcgtt tggtaatttc
    20641 gcgcatttca
//
LOCUS       DASSGQ010000300         4135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_444795,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000300 DASSGQ010000000
VERSION     DASSGQ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444795"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="VFI55_13280"
     CDS             complement(<1..1247)
                     /locus_tag="VFI55_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HET7075038.1"
                     /translation="MTPATTSHDLSDHELALVDKYWRAANYLSVGQIYLLDNPLLTEP
                     LSPEHVKPRLLGHWGTTPGLNLIYVHLNRIIRNRDANIIYVTGPGHGGPGLVANAYLE
                     GTYSEVYTGIEEDAEGMRKLFRQFSFPGGIPSHVAAQTPGSIHEGGELGYALVHAYGA
                     AFDNPDLVVACVVGDGEAETGPLAASWHSNKFLNPVKDGAVLPILHLNGYKIANPTVL
                     ARIPHRELESLLRGYGYRPIVVAGDDPANVHLQLAAALDDAFDDIAAIQGTARGGNAL
                     ERPVWPMIVLRTPKGWTGPKVVDGKKVEGTWRAHQVPLAETRGNPAHRVQLQEWLRSY
                     RPEELFDGDGRLRPELRAMAPRGDRRMSANPHTNGGTLLHDLDLPDFREYAVPVPQPA
                     TSTHEATRILGTFLRDVIARNPDR"
     gene            complement(1315..2163)
                     /locus_tag="VFI55_13285"
     CDS             complement(1315..2163)
                     /locus_tag="VFI55_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7075039.1"
                     /translation="MADTASTGLKVREKVIVITGGARGIGLATATALHNLGARVAIGD
                     VDEVRVKESGTALGLDVYGKLDVTDSQSFSEFLDDVERQLGPIDVLVNNAGIMPLGRI
                     IDEPDALTRRILDINVYGVIVGTKLAVQRMVPRGSGHVINVASLAGEGSIPGAATYCA
                     SKHAVVGFTDTARLEYRKTGVKFSIVLPTFVNTELVAGTTGAKGFKNAEAADIADAIV
                     KLVVHPKPRVRVTKVAGAVVVSAKFMPRPVAEGINRMLGGEHFFTDDVDVAKRKAYEA
                     RARGTG"
     gene            2293..2802
                     /locus_tag="VFI55_13290"
     CDS             2293..2802
                     /locus_tag="VFI55_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7075040.1"
                     /translation="MTKVRKAVPADADEVARLHVRSWQSAYRGLLGSEYLDGLRPEVW
                     AARYSFGRVGLRMPSTLVAVDGTAIRGLVTTGLARADDIPSFGELMAIYVDPAYTGTG
                     VGRLLITAARARLRLVGVPGVLLWVLDGNVGARRFYERDGWGFDGTCRTAIFGDTPVR
                     LMRYRCAPV"
     gene            complement(2799..3716)
                     /locus_tag="VFI55_13295"
     CDS             complement(2799..3716)
                     /locus_tag="VFI55_13295"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET7075041.1"
                     /translation="MSAHLSYVEAVLVGAFQGVTELFPVSSLGHAVVVPALIGGQWAQ
                     DLSVSTPESPYLAFIVGLHVATAAALLVFFWRDWLRILAGFASSLRYRRIHTPDERLA
                     WLIVVGTIPVGLAGLALEHTFRTTLGKPIPAAAFLVLNGIVMYAGEVLRRRVAPAAGP
                     GQTTPPDALHTDEAIDNRLAQLPMLRGAVVGSSQILALLPGISRSGIAMVAGLWRGLS
                     HEDAARFSFLLATPIILAAGVYKIPDLFGPLGAGIHGQVLAGSIASFVCAYLAVRFLT
                     RYFQTRTLTPFAVYCVAAGGASLAWLTLR"
     gene            3945..>4135
                     /locus_tag="VFI55_13300"
     CDS             3945..>4135
                     /locus_tag="VFI55_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET7075042.1"
                     /translation="MQMTDGLLDAVRVLDLSSGDADAVTRLFADLGADVLKVEPPGGN
                     LAREELPTVADAGIPFAMH"
BASE COUNT          668 a         1339 c         1435 g          693 t
ORIGIN      
        1 cggtccgggt tgcgggcaat cacatcgcgc aggaaggtgc ccagtatccg ggtggcctcg
       61 tgcgtcgagg tggccggttg cggcaccggc acggcgtact cgcggaaatc tggcaggtcg
      121 aggtcgtgca gcagcgtccc gccgttggtg tgcgggttgg cgctcatccg gcgatcaccc
      181 cggggcgcca tcgcccgcag ctcggggcgt aggcggccgt cgccgtcgaa caactcctca
      241 ggccggtagc tgcgcagcca ctcctgcagc tgcacgcgat gtgcgggatt gccgcgtgtt
      301 tcggccagcg gcacctggtg cgcacgccag gtgccctcca ccttcttgcc gtcgaccacc
      361 ttcggcccgg tccagccctt cggggtgcgc aacacgatca tcggccacac gggtcgctcc
      421 agcgcgttgc cgccgcgtgc cgtgccctgg atggccgcga tgtcgtcgaa ggcgtcgtcg
      481 agggcggcgg cgagctgcag gtgcacgttg gcgggatcgt caccggccac cacgatgggc
      541 cggtagccgt agccgcgcaa cagcgattcc agttcgcggt gtgggatgcg cgccagcacc
      601 gtcgggttgg cgattttgta gccgttgagg tgcaggatgg gcagcaccgc gccgtctttg
      661 accgggttga ggaacttgtt cgagtgccag ctggcggcca acggcccggt ttcggcctca
      721 ccgtcgccga ccacgcaggc caccaccagg tccggattgt cgaacgccgc gccgtaggcg
      781 tgcaccaacg cgtacccaag ctcgccgcct tcatggatgg aacccggcgt ctgggccgcg
      841 acgtgactgg ggatgccgcc gggaaacgag aactgccgga acagcttccg cattccttcg
      901 gcgtcttcct cgatgccggt gtacacctcg ctgtaggtgc cctccaggta ggcgttggcg
      961 accaatcccg ggccgccgtg accgggcccc gtcacgtaga tgatgttggc gtcgcggttg
     1021 cggatgatcc ggttcaggtg cacatagatg aggttgagcc ctggcgtggt gccccagtgc
     1081 cccaacagtc ggggtttgac gtgctcaggc gacagcggct cggtcagcag cgggttgtcc
     1141 agcaggtaga tctggccaac cgagaggtaa ttggcggcgc gccagtactt gtcgacgagg
     1201 gccagctcgt ggtcggagag atcgtgcgag gtcgttgctg gagtcatcgt gtgttccgga
     1261 ctcatctgcc cgattgtgcg tgccccgaat gaacggcagc cgacgcgcga gtgctcaccc
     1321 tgtgccgcgc gcccgggcct cgtaggcctt gcgcttggcg acgtcgacgt catcggtgaa
     1381 gaagtgctct ccgccgagca tccggttgat gccctccgcg acggggcgcg gcatgaactt
     1441 cgccgaaacg accaccgcac cggccacttt cgtgacccgt acccgtggct tggggtggac
     1501 caccagcttc acgatcgcgt cggcaatgtc ggcggcctcg gcgttcttga accccttcgc
     1561 ccccgtggtg ccggcgacga gttcggtgtt gacgaacgtc ggcaacacga tcgagaattt
     1621 cacgccggtc ttccggtatt cgagcctggc ggtgtcggtg aagccaacca ccgcgtgctt
     1681 gctggcgcag taggtcgccg cgccggggat actcccttcc ccggcgagcg aggcgacgtt
     1741 gatgacgtgt ccggatccgc gcgggaccat gcgctgcacc gccaacttgg tgcccacgat
     1801 cacgccgtag acgttgatgt ccagaatgcg ccgggttagc gcgtccggct cgtcgataat
     1861 ccggccaagc ggcatgatgc cggcgttgtt gaccagcaca tcgatcgggc cgagttggcg
     1921 ctcgacatcg tccaggaact ccgaaaagga ctgcgagtcg gtgacatcca gcttcccgta
     1981 gacatcgagg ccgagcgcgg tcccggactc cttcacccgc acctcgtcga cgtcgccgat
     2041 cgcgactcgg gcacccaagt tgtgcaacgc ggtcgcggtg gccagcccga ttccccgcgc
     2101 gccgccagtg atcacgatta ccttttcgcg gaccttgagg ccggtggatg cggtgtctgc
     2161 cattttcgtc cttccctggg gttgacgggt gtcaattggc gattcgctaa gcacagcact
     2221 cgacgtcgcg cagcgcaaat gaccgatggg tcgacttatg acgagccggc cggccgcgtc
     2281 aatactgcaa caatgaccaa ggtgcggaag gccgtcccgg cggacgccga cgaagtggcc
     2341 cgtctgcacg tccggtcgtg gcagtccgcg taccgaggac ttctcggctc cgagtacctc
     2401 gacggcctca ggcccgaggt ctgggccgcg cgatacagct ttggtcgcgt gggcctgcgg
     2461 atgccgtcca ccttggtcgc ggttgacggt accgcgatcc gtggcttggt gaccacgggt
     2521 ctggcgcgcg ccgacgacat accgagtttc ggcgagctga tggcgatcta cgtcgatccg
     2581 gcgtacacgg gcaccggggt agggcgccta ctcatcaccg ccgcccgggc gcggctgcgc
     2641 ctggtcgggg tgccgggggt cctgctgtgg gtgctagacg gcaatgtcgg agcccggcgg
     2701 ttttacgaac gcgacggatg gggcttcgac ggtacgtgtc gcacagcgat tttcggcgac
     2761 acaccggtgc ggctgatgcg ctatcgatgc gcgcccgttt agcgcagggt gagccaagcc
     2821 aggctggcgc cgccggccgc cacacagtag acggcgaacg gtgtgagggt gcgggtctgg
     2881 aagtaccgcg tgagaaagcg cacggcaagg taggcgcaga cgaacgaggc gatgctgccg
     2941 gccagcacct ggccgtggat cccggctccc aggggcccga atagatccgg gatcttgtag
     3001 accccggcgg ccaggatgat gggcgtcgcc agcaaaaagg agaagcgggc ggcgtcctca
     3061 tgcgacaggc cgcgccacag gccggcaacc atggcgatgc cggatcggct gatcccggga
     3121 agcagggcca ggatttggga ggagccgacc actgccccgc gcagcatcgg cagctgagcc
     3181 agccggttgt cgatcgcctc atcggtgtgt agcgcgtcgg gcggcgtggt ctggcctgga
     3241 cccgccgcag gcgcgacgcg tcggcgcagg acttcaccgg catacatgac aatgccgttg
     3301 agcaccagga acgcggcggc cggaatgggc ttgcccaatg tggtgcggaa ggtgtgttcc
     3361 agcgccagtc cggccagccc caccgggatg gtgcccacca cgatcagcca ggcaagccgc
     3421 tcgtcgggag tgtggattcg ccggtaccgc agcgacgagg cgaagccggc gagaatgcgc
     3481 agccagtcac gccagaagaa caccagcagg gcggcggcgg tagcgacatg caggccgacg
     3541 atgaacgcca ggtacggcga ttcgggggtc gagacgctga gatcctgagc ccattgcccg
     3601 ccgatcagcg ccggcaccac caccgcatgc ccgaggctgg agacggggaa cagctcggtg
     3661 acgccctgga atgcgccgac cagcacggcc tcgacgtagc tcaagtgcgc gctcacgggt
     3721 tgggtgcctc cctttgacgg tctgcgatac gacctcatgg tcggcctgtg accttagccg
     3781 acgccatcat caccaacatc cccgaccaag acagggggcg catctgatcg tgccttcatc
     3841 gaatcacact gcccggcttg gcttttggcc gaactctagg caccagccgg gttgcccgca
     3901 gggcaggcgc ccgcgaaatg ggcacctgac caggcaggta cggtgtgcag atgacagacg
     3961 ggctgctcga cgccgtgcgc gtgctcgact tgtccagcgg tgatgccgac gcggtgaccc
     4021 gcctgttcgc ggacctgggc gccgacgtcc tcaaggtgga acccccgggc ggaaatctgg
     4081 cacgcgaaga gttgccgacg gtggccgacg ccggcattcc gttcgcgatg cacaa
//
LOCUS       DASSGQ010000301         8057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1510446,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000301 DASSGQ010000000
VERSION     DASSGQ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8057
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510446"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VFI55_13305"
     CDS             complement(<1..451)
                     /locus_tag="VFI55_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HET7075043.1"
                     /translation="MNNLYRNLAPVTEVAWAEIEVEATRTFKRHIAGRRVVDVSGPSG
                     PVTAAVGTGRLIDVEAPTDGVVAHMRASKPLVRLRVPFTLSREEIDNVERGSQDADWD
                     PVKEAAKKLAFVEDRTIFEGYAAADIEGTRGASSNKPLTLPEDPREIP"
     gene            complement(451..1467)
                     /locus_tag="VFI55_13310"
     CDS             complement(451..1467)
                     /locus_tag="VFI55_13310"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HET7075044.1"
                     /translation="MPSVQPQPILAPLTPAAIFLVVTINEGGETTVHESLPDLSGLVR
                     AIGFRDPAKRLSAITSIGSEAWDRLFSGSRPAELHPFIELTGPRHTAPATPGDLLFHI
                     RAETMDVCFELAGRILKSMAGAITVVDEVHGFRFFDNRDLLGFVDGTENPDGTVAVSA
                     TAIGDEDPEFAGSCYVHVQKYVHDMSSWESLSVTEQQLVIGRTKLEDIELDDDAKPAN
                     AHIALNVIEDEDGNELKIVRHNMPFGEVGKREYGTYFIGYSRTPEVTERMLRNMFLGD
                     PPGNTDRILDFSVAETGGLFFSPTVDFLDDPPPLPAPLVTTPPATPASDDGSLSIGSL
                     KGTS"
     gene            1649..2947
                     /locus_tag="VFI55_13315"
     CDS             1649..2947
                     /locus_tag="VFI55_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019695270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HET7075045.1"
                     /translation="MVIVSGGPTTSASARGLCDFIDASPSPFHVCATVSGRLVEAGYA
                     ELSETDRWPDQPGRYFTVRAGSLVAWNSDGPDGPFRIVGAHTDSPNLRVKQHPDRLVA
                     GWRVVALQPYGGAWLNSWLDRDLGISGRLSVRPGGQGAGISHRLVRIDDPILRVPQLA
                     IHLAEDRKSLTLDPQRHVNAVWGVGTQTRSFLGYVAERAGVAPADVLAADLMTHDLTP
                     STLIGPDADLLGAPRLDNQASCYAGMAALLSAEPRGFLPVLVLFDHEEVGSSSDRGAQ
                     SNLLSIVLERIVLAAGGNREDFLRRLPASLLASADMAHATHPNYPERHEPGHPIGGNA
                     GPVLKVHPNLRYATDGRTAAAFELACRQAGVPLQRYEHRADLPCGSTIGPLASARTGI
                     PTVDVGAAQLAMHSARELMGAHDVAAYSAALQAFLSPEPF"
     gene            2966..3313
                     /locus_tag="VFI55_13320"
     CDS             2966..3313
                     /locus_tag="VFI55_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015626911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7075046.1"
                     /translation="MSLNVEMVTFDCSDPATLGQWWADQFGGTTHELLADEFTAVTLP
                     EGPRLGFQKVPDPTPGKNRVHLDFGAADVDAEASRLTAAGATEVGRHTFGENFRWVVL
                     ADPEGNVFCVVGQ"
     gene            complement(3322..3747)
                     /locus_tag="VFI55_13325"
     CDS             complement(3322..3747)
                     /locus_tag="VFI55_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7075047.1"
                     /translation="MTDTRTTPDRTNADSEAAKFSAEVFAAFWAAPDLSHGLDVLADD
                     IIGYWPGDPEPVRGIEAYTAKLVELLDAAPDLSLEVVDSATVPGNAAGEELVFLHYVG
                     RGTGPSGPFDIRGIDRVRTRDGIVVENVIRYEPVFVAQH"
     gene            3873..4661
                     /locus_tag="VFI55_13330"
     CDS             3873..4661
                     /locus_tag="VFI55_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7075048.1"
                     /translation="MSPAPVGVLLREWRARRRVSQLDLSLSVGVSARHLSFIETGRSR
                     PSPEMVLALADGLDVPLRERNALLLAAGFAPRYQSRPLEDDALSPARDAVQRLLDAHD
                     PYPGVVIDRCWNIVGANAAASALIAGLPQELLGPPANIYRLSLHPDGLAGRTLNFAEW
                     AGYLLHQLRRAVALTGDPVLRALDMEVRGYPGVEGAAREWGTPRDNAALLVPFVLDIG
                     DGRRLSMFTTLTTFGTPLDVTLAELAVELFYPADAESAGLLRGG"
     gene            4713..6977
                     /gene="purL"
                     /locus_tag="VFI55_13335"
     CDS             4713..6977
                     /gene="purL"
                     /locus_tag="VFI55_13335"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HET7075049.1"
                     /translation="MIDTVEHAATTPDQPQPFGELGLKDDEYQRIREILGRRPTDAEL
                     AMYSVMWSEHCSYKSSKVHLRYFGETTTDQMRAGMLAGIGENAGVVDIGDGWAVTFKV
                     ESHNHPSYVEPYQGAATGVGGIVRDIMAMGARPVAVMDQLRFGAADAPDTRRVLDGVV
                     RGIGGYGNSLGLPNIGGETVFDACYAGNPLVNALCVGVLRQEDLHLAFASGAGNKIIL
                     FGARTGLDGIGGVSVLASDTFDSSGSRKKLPSVQVGDPFMEKVLIECCLELYAGGLVI
                     GIQDLGGAGLSCATSELASAGDGGMAIQLDTVPLRAKQMTPAEVLCSESQERMCAVVA
                     PENVDEFMAVCRKWEVLATVIGEVTDGDRLRITWHGETVVDVPPRTVAHEGPVYHRPV
                     VRPESQDALNADTSAGLPRPATGDELRATLLALLGSPHLCSRAFITEQYDRYVRGNTV
                     LAEHSDGGVLRVDESSGRGIAVSTDASGRYTLLDPYAGAQLALAEAYRNVAVTGATPV
                     AVTNCLNFGSPEDPGVMWQFSQAVRGLADGCAALGIPVTGGNVSFYNQTGSAAILPTP
                     VVGVLGVIDDVGRRIPTGLGTEPGETLLLLGDTRDEFDGSVWAQVTAEHLGGVPPAVD
                     LAREKLLADVLSSASRDGLVSAAHDLSEGGLAQAIVEAALAGETGCRIVLPEGTDPFV
                     TLFSESAGRVLVAVPRTEESRFRSMCEARGLPAIRIGVVDQASDAVEVQGLFTVSLAE
                     LRATSESVLRRFFG"
     gene            6996..7664
                     /locus_tag="VFI55_13340"
     CDS             6996..7664
                     /locus_tag="VFI55_13340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET7075050.1"
                     /translation="MPDRRFRRLRAFSLAAALVGWSFVGPRLPIACRAPLQAGVGALL
                     VSVTRAPLGLCPPRLWAGVRLGSAAGVLASSAIAATTAAPRVRLSMAAQEASASAPAW
                     LLWRIPVGTVWAEEAAFRAALATEGFRAFGKRGGRLLQATAFGLSHIPDARATGAPVV
                     PTVLVTGLAGWLFGWLAERSGSLAAPMLAHLATNETGAIAALIVRRGGWNSSQVHRSN
                     PSPR"
     gene            7715..>8057
                     /locus_tag="VFI55_13345"
     CDS             7715..>8057
                     /locus_tag="VFI55_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7075051.1"
                     /translation="MHRLRAAEHPRPDYVLLHISDTHLVSDGSLYGAVDADGRLGELL
                     EQLKHSGVRPDAIVFTGDLADTGEPEAYRKLRAVVEPFADALGAELIWVMGNHDDRAA
                     LRSWLLDEAPSM"
BASE COUNT         1193 a         2626 c         2829 g         1409 t
ORIGIN      
        1 cggggatttc gcgcgggtcc tcgggtagcg tcagcggctt gttcgagctg gcgccgcggg
       61 tgccctcgat gtccgcagcg gcgtaaccct cgaagatggt gcggtcctcc acgaaggcca
      121 gcttcttggc ggcctccttg accgggtccc agtccgcatc ctgcgaaccg cgctcgacgt
      181 tgtcgatttc ctcgcgtgac agcgtgaacg gaacccgtag ccggacaagc ggtttgctgg
      241 cccgcatgtg ggcgaccacg ccgtcggtgg gcgcctcgac gtcgatcagg cgaccggtgc
      301 ccaccgccgc ggtgaccgga ccgctgggcc cgctgacgtc gacgacgcgg cgcccggcga
      361 tatggcgttt gaacgtccgg gtggcctcca cttctatttc ggcccaggcg acttcggtga
      421 ccggtgccag gttgcggtaa aggttgttca tcacgaggtt cctttcaggc tgccgatcga
      481 aagtgagccg tcatcagaag cgggagtggc tggcggcgtt gtgaccagtg gtgcgggcag
      541 gggcggtggg tcgtcgagga agtcgacggt gggcgagaag aacagtccgc cggtttccgc
      601 gacggaaaag tccagaatgc gatcggtgtt gccgggcgga tcgcccagga acatgttccg
      661 cagcattcgc tcggtgacct ccggcgtgcg cgaatacccg atgaaatatg tgccgtactc
      721 acgcttgccg acctcgccga acggcatgtt gtgccgcacg atcttcagct cgttgccgtc
      781 ctcgtcttcg atcacgttca gcgcgatgtg cgcgttggcc ggcttggcgt cgtcatcgag
      841 ttcgatgtct tccagcttcg tccggccgat gacgagttgc tgctcggtga ccgaaagcga
      901 ctcccacgac gacatgtcgt gcacgtattt ttgcacgtgg acataacacg aacccgcgaa
      961 ttcggggtcc tcgtcaccga tggcggtggc gctcacagcc actgtgccat cggggttttc
     1021 ggtgccgtcg acgaagccca gcaggtcgcg gttgtcgaag aagcggaagc cgtggacctc
     1081 gtcgacaacg gtgatcgctc ccgccatcga cttgaggatg cggcccgcca actcgaaaca
     1141 gacatccatg gtttcggcac ggatatggaa caggaggtcg ccgggtgtgg ccggggcggt
     1201 gtgccgcggt ccggtcagct cgatgaacgg gtgcagttcg gcgggccgtg acccgctgaa
     1261 caaccggtcc caagcttcgg agccgatcga cgtgatcgcc gacaagcgct tcgcgggatc
     1321 gcgaaagccg atcgcgcgca ccagccctga caagtccggt agcgattcgt ggaccgtcgt
     1381 ttcgccgcct tcgttgatgg tgaccacgag gaagatcgcg gcgggcgtca acggcgcgag
     1441 gatgggttgc ggctgaaccg aaggcacgct gccgacctta gcgtggcgac ggcgagcgct
     1501 ggaaccggtg cgagtaagga gccgggcaat ccggcccagc gtggcaacgg cgagcgctgg
     1561 aaccggtgcg gtgtgggggt ccccccgctt gcggggggag agccgggcaa tcgggcccag
     1621 cgtgactcct ccatcacgga cagccatgat ggtcatcgtg tccggcgggc cgaccacctc
     1681 agccagtgca cggggcctgt gcgatttcat cgacgcgtct ccgtcgccgt ttcatgtctg
     1741 cgccacggtg tccggtcgac tggtggaagc cgggtatgcc gaactcagcg aaaccgaccg
     1801 ctggccggac caaccgggcc gctatttcac cgtccgcgcc ggttcattgg tcgcctggaa
     1861 ctctgatggg cccgacggtc cgttccggat cgtcggcgcg cacaccgaca gcccgaatct
     1921 gcgcgtcaag cagcatccag accggctggt cgccggttgg cgggtggtgg ccctgcagcc
     1981 ctacgggggc gcgtggctca actcgtggct ggaccgggat ctgggtatca gtgggcgact
     2041 gtcggtgcgt ccgggcggtc aaggcgccgg gatttcccat cggctggtcc ggatcgacga
     2101 ccccatcctg cgggtgcccc agctggccat ccatctggcc gaggaccgca agtcgctgac
     2161 gctggacccg cagcgtcacg tcaacgcggt ctggggtgtc ggcacacaaa cacggtcgtt
     2221 ccttggttac gtcgcagagc gcgccggggt agcgccggcc gatgtgctgg ccgccgatct
     2281 gatgacgcat gatctgaccc cgtcgacgtt gatcggcccc gacgccgacc tgttgggcgc
     2341 accccggctg gacaatcagg ccagttgcta tgcggggatg gcggcgctgc tgagcgccga
     2401 gccccggggt tttctgcccg tgttggtgct cttcgatcac gaggaggtcg gttcgtcctc
     2461 cgaccgcggc gcccagtcca acctgttgag catcgtcctg gaacggattg tgctggcggc
     2521 cgggggcaac cgggaagatt tcctgcgccg gctgccggcg tcgctgctgg cctcggcgga
     2581 catggcgcac gccacccatc ccaactatcc ggagcggcac gagccgggcc acccgatcgg
     2641 gggcaatgca gggcccgtgc tcaaggtgca tccgaatctt cggtacgcca ccgacggacg
     2701 caccgccgcc gcgttcgagc tggcctgccg ccaggccggg gtcccgttgc agcgttacga
     2761 gcaccgcgcc gacctgccgt gcggttccac gatcggaccg ctcgcctcgg cacggaccgg
     2821 tatccccacg gtggacgtcg gcgcggccca gctagcgatg cattccgcgc gagagttgat
     2881 gggcgcccac gacgtggccg cctattcggc cgcgctgcaa gcgtttctgt cgcctgagcc
     2941 gttttgaggc ataaggtcga gcgatatgag tctcaacgtg gagatggtca ccttcgactg
     3001 cagcgatccc gcgacgctgg ggcaatggtg ggccgaccag ttcggtggta cgacgcacga
     3061 gctgctcgcc gacgagttca ccgcggtgac attgccggaa ggaccccggc tcgggttcca
     3121 gaaggtgccc gaccccaccc ccgggaaaaa tcgtgtacac ctcgacttcg gtgcagccga
     3181 tgtcgatgcc gaagcgtcgc ggctgacggc ggccggggcg accgaggtgg gccggcacac
     3241 gtttggagag aacttccgct gggtagtgct ggccgacccc gaaggcaacg tcttttgcgt
     3301 ggtggggcag tagctcgccg ctcagtgctg ggcgacgaaa accggctcgt aacggatgac
     3361 gttctccacg acaatcccgt cgcgagtgcg aacccggtcg atcccgcgga tgtcaaacgg
     3421 tccggacggg ccggtgcccc ggccgacgta gtgcaagaac accagttcct cgcccgctgc
     3481 gttgccggga accgttgcac tgtcgacgac ctcaagcgac agatccggcg ccgcgtcgag
     3541 cagttcgacg agtttcgccg tgtatgcctc gatcccgcgg accggctccg ggtcgcccgg
     3601 ccagtagccg atgatgtcgt cggccaggac atccaacccg tgcgacaagt cgggtgcggc
     3661 ccagaacgcc gcgaacacct cggcgctgaa tttcgcagct tctgagtcgg cattcgtgcg
     3721 gtccggggtg gttcgggtgt cggtcatggt ttctcctcgg gtagagcgct gcgatcgagc
     3781 gcgtcgcagc aagcgtgtct cgccgcaccg cggcggctca attacctccg aggtcatcgc
     3841 tatgggtgcg tcggcgtggt taccgttgct tcgtgagccc cgcaccggtc ggtgtcctgc
     3901 tgcgcgaatg gcgtgcgcgt cgacgggtga gccagctcga cctgtcgctg agcgtgggtg
     3961 tgtcggcgcg gcatctgagc ttcatcgaga ccgggcgttc ccgtcccagc cccgagatgg
     4021 tgctcgctct cgccgacggc ctcgacgtgc cgctgcgaga gcgcaacgcg ctgctgctgg
     4081 cagccgggtt cgccccgcgc taccagtcac gcccactgga ggatgacgcg ctgtctccgg
     4141 cccgggacgc ggtgcagcgc ctcctcgatg cgcacgaccc atacccgggc gtggtcatcg
     4201 accggtgctg gaacatcgtg ggcgccaacg ccgccgcgtc cgccttgatc gccggcctgc
     4261 cacaggagct gctcggcccg ccggccaaca tctaccggtt gtccctgcac cctgacggcc
     4321 tcgccgggcg gacgctgaat ttcgccgaat gggccggcta tctgctgcat cagctccgac
     4381 gcgcggtcgc gctcaccggc gacccggtac tgcgggccct cgacatggag gtgcgcggat
     4441 atccgggcgt cgagggtgcc gcaagagagt ggggcacgcc acgggacaac gccgcgctac
     4501 tcgtgccgtt cgtgctcgat atcggcgatg ggcgacggct gtcgatgttt accacgctga
     4561 cgacattcgg gactccgctc gatgtcaccc tcgccgaact cgccgtcgag ttgttctacc
     4621 cggccgacgc cgaaagcgcc ggtctgctcc gcggtgggta gctctttaga ctgttctgcg
     4681 tgacggtctc cggtacaagc gcgcgcaccc agctgattga caccgtcgag cacgccgcga
     4741 ccactcccga ccagccgcag ccattcggcg agctgggcct caaagacgac gagtaccagc
     4801 ggattcgcga gatcctgggc cgccggccaa ccgacgccga gttggcgatg tactcggtga
     4861 tgtggagcga gcactgttcc tacaagtcct ccaaggttca cctgcgctac ttcggtgaga
     4921 cgaccaccga ccagatgcgc gccggcatgc tggccggcat cggcgagaac gccggcgtcg
     4981 tcgacatcgg cgacggctgg gcggtcacgt tcaaggtgga atcgcacaac cacccgtcct
     5041 acgtcgagcc gtatcagggc gcggccaccg gcgtcggtgg cattgtccgc gacatcatgg
     5101 ccatgggtgc ccgaccggtc gccgtgatgg accagcttcg attcggtgcc gccgatgcgc
     5161 cggacacccg ccgcgtgctc gacggcgtgg tccgtggcat cggtggctac ggcaactcgc
     5221 tgggcctgcc aaacatcggc ggcgagaccg tcttcgacgc gtgctatgcc ggcaacccgt
     5281 tggtcaatgc gctctgcgtc ggcgtattac gccaggagga cctgcatttg gcgttcgcct
     5341 cgggcgccgg caacaagatc attcttttcg gcgcgcggac gggcctggac ggcatcggcg
     5401 gggtgtcggt gctggcgtcg gacactttcg acagctcggg atcccgcaag aagctgccgt
     5461 ccgttcaggt cggtgacccg ttcatggaga aagtgctcat cgagtgctgt ctggagctgt
     5521 acgcgggcgg gctggtgatc ggcatccagg acctcggcgg agctggattg tcctgtgcca
     5581 cctcggagtt ggcgtcagcc ggagacggcg ggatggcgat tcagctggac accgttccgc
     5641 tgcgggccaa gcagatgaca cccgccgagg tgctctgcag cgagtcccag gagcggatgt
     5701 gcgcggtggt agcaccggag aacgtggacg agttcatggc ggtgtgccgc aagtgggagg
     5761 tgctggccac cgtcatcggc gaggtcaccg acggcgaccg gttgcggatc acctggcacg
     5821 gcgagacggt ggtcgacgtg ccgccgcgca ccgtggccca cgaaggcccg gtgtaccacc
     5881 gtccggtggt ccgccccgaa tcgcaggacg ccctgaacgc agacacctcg gccggtctgc
     5941 cgcggccggc gaccggggat gagctgcgcg cgactttgct tgcgctgctg ggcagtccgc
     6001 acctgtgcag ccgcgcgttc atcaccgagc aatacgaccg ctatgtgcgt ggcaacaccg
     6061 tgctggccga gcactccgac gggggtgtgc tgcgcgtcga cgagtcctcc ggccgcggca
     6121 tcgcggtgtc gaccgacgcg tcggggcgct acacgctgct ggatccctac gccggtgccc
     6181 agctcgcgct ggccgaggcc tatcgcaacg ttgccgtcac cggcgccacc ccggtcgcgg
     6241 tgaccaactg cctcaatttc ggttcgcccg aagaccccgg ggtgatgtgg cagttttcgc
     6301 aggcggtgcg cggcctggcc gatggctgtg cggcccttgg tatcccggtg accggcggaa
     6361 acgtgagctt ttacaaccag accgggtcgg cggcgatcct gcccacgccg gtggtcggcg
     6421 tgctgggcgt gatcgacgat gtcggcaggc gcattcccac cggcctgggc accgaaccgg
     6481 gcgaaaccct gttactgctc ggtgatacgc gcgacgaatt cgacgggtcg gtctgggcgc
     6541 aggtcaccgc ggagcatctc ggtggggtgc ctcccgcggt cgatctggcc cgcgaaaagc
     6601 tactggccga cgtgctgagt tcggcgtcgc gcgacgggct ggtgtccgcc gcgcacgacc
     6661 tgtccgaggg cggcctcgcg caggccatcg tggaagcggc gctggccggt gaaaccggtt
     6721 gccgcatcgt gcttcccgag ggcacagatc cattcgtgac gttgttctcc gagtcggcgg
     6781 gccgggtgtt ggtggcggtg ccgcggaccg aggagagccg gttccgttcg atgtgcgagg
     6841 cgaggggact gcccgcgatc cgcatcgggg tcgtcgacca ggcttcggac gcggtggagg
     6901 ttcagggcct gttcacggtg tccctggcag aactgcgcgc gacgtccgag tcggtgctgc
     6961 ggcgattctt cggatgagcg cagccgccaa acccgatgcc tgacagacgt tttcgtcgcc
     7021 ttcgggcgtt ctcgttggcc gcggcgctgg tcggctggag ttttgtcggc ccgcggctcc
     7081 ccattgcgtg ccgggcgccg ctgcaggccg gggtgggcgc actgctggtg tcggtgaccc
     7141 gcgcgccgct ggggctgtgc ccgccgcggc tgtgggcggg ggtgcggctg ggatcggcgg
     7201 ccggtgtgtt ggcatcgagc gcgatcgccg cgacgaccgc ggcgccgcgg gtgagactgt
     7261 cgatggcggc ccaagaggcg tcggcgtccg cgccggcctg gctgctctgg cggatacccg
     7321 tcggcaccgt ctgggccgag gaggccgcct tccgcgcggc gctggccacc gaggggtttc
     7381 gtgccttcgg caagcgcggc ggaaggctgc tgcaggccac cgctttcggg ctctcccaca
     7441 tcccggacgc ccgggcgacg ggtgcgccgg tggtgcccac cgtgctggtc acgggcctcg
     7501 ccggctggct gttcggctgg ctggccgagc gctccggcag cctggccgcg ccgatgctgg
     7561 cgcatttggc caccaacgag accggtgcga tcgctgcgct gatcgtcagg cgcggcgggt
     7621 ggaacagctc acaagtgcac agatctaatc cgtcaccccg ctagacttgt taattcactg
     7681 ttaaatcgat cgatggagag ggttggcacc tcaggtgcac agactgaggg ccgcggagca
     7741 cccgcggccg gattacgttc tcttacacat cagcgacacc catctcgttt cggatgggtc
     7801 gctctacggc gcggttgacg ccgacggcag gctgggcgaa ctgctcgagc agctcaaaca
     7861 ctccggggtg cgtcccgacg cgatcgtatt caccggcgat ctggccgata ccggtgagcc
     7921 cgaggcctat cggaaacttc gcgccgttgt ggagcccttt gcggacgcgt tgggcgccga
     7981 actcatctgg gtgatgggca accacgacga ccgggccgcg ttgcgcagct ggctgctgga
     8041 cgaagcgccg tcaatgg
//
LOCUS       DASSGQ010000302        20226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_325997,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000302 DASSGQ010000000
VERSION     DASSGQ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 20226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20226
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325997"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..986)
                     /locus_tag="VFI55_13350"
     CDS             complement(60..986)
                     /locus_tag="VFI55_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET7075052.1"
                     /translation="MPEDSHEALDAYSAVVTAVANAVLPSVASLAVHTRRGDGSGSAS
                     VITPDGYLLTSAHVVAGAGSAEATFSDGTSVAADVTGRDELSDLAVLKARGNVPSPVI
                     MGRAEDLVVGQLVVAVGNPMGLAGSVTAGIVSGLGRSLPTRNGRVVDEVIQTDAALNP
                     GNSGGVLADSRGRMVGVSTAVAGVGLGLAVPINASTHKIIAALMHTGRVRRAWLGIAG
                     AHVSVPPAVAAKLGTAHGLQVASVVAGSPAAEAGVRRGDIVVSVDGQRIVTATAIQQL
                     MVEDAIARRIEMTVWRNGALVDVFVVPRELAG"
     gene            complement(1046..2557)
                     /gene="gatB"
                     /locus_tag="VFI55_13355"
     CDS             complement(1046..2557)
                     /gene="gatB"
                     /locus_tag="VFI55_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET7075053.1"
                     /translation="MSVATKAELLDYDDVIARFDPVLGLEVHVELSTVTKMFCGCATT
                     FGAEPNTQVCPVCLGLPGSLPVLNRAAVQSAIRIGLALNCEIVPWCRFARKNYFYPDM
                     PKNYQISQYDEPIAINGYLEAPLEDGTTWRVEIERAHMEEDTGKLTHLGSETGRIHGA
                     TTSLIDYNRAGVPLIEIVTKPIVGAGAQAPQIARAYVTALRDLLRVLEVSDVRMDQGS
                     MRCDSNVSLKPIGTAEFGTRTETKNVNSLKSVEVAVRYEMQRQGAVLTSGGRIIQETR
                     HFHEAGYTSPGRAKETAQDYRYFPEPDLEPVAPSREWVEQLRQTIPELPWLSRKRIQQ
                     DWGISDEVMRDLVNAGAVELVIETVKHGASSEHARAWWGNFLVQKANEASIGLDELAI
                     TPAQVAAVVALVDEGRLSNKLARQVVEGVLAGEGEPEQVMTARGLALVRDDSVTQAAV
                     DEALAANPDVAEKIRGGKVAAAGAIVGAVMKATRGQADAARVRELVLAACGQG"
     gene            complement(2619..3650)
                     /locus_tag="VFI55_13360"
     CDS             complement(2619..3650)
                     /locus_tag="VFI55_13360"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HET7075054.1"
                     /translation="MRIGILTGGGDCPGLNAVIRAVVRTCDSRYGSSVVGFQDGWRGL
                     LENRRMQLKNDDRNDRLLVKGGTMLGTARVHPDKLRAGLHQIKQTLDDNGIDVLIPIG
                     GEGTLTAAHWLSEENVPVVGVPKTIYNDIDCTDVTFGHDTALTVATDAIDRLHSTAES
                     HQRVMLVEVMGRHAGWIALNAGLASGAHMTLIPEQPFDVEEVCRLVKRRFQRGDSHFI
                     CVVAEGAKPIAGSITLREGGLDEFGHERFTGVAQQLGVAVERRINKEVRVTVLGHVQR
                     GGTPTAYDRVLATRFGVAAADAAHAAEYGQMVSLRGQDIGRVQLADATRQLKLVPESR
                     YDDAAAFFG"
     gene            complement(3747..5231)
                     /gene="gatA"
                     /locus_tag="VFI55_13365"
     CDS             complement(3747..5231)
                     /gene="gatA"
                     /locus_tag="VFI55_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET7075055.1"
                     /translation="MSDIVRSDAATLAASIAAKELSSTEITQACLDQIEATDARYRAF
                     LHVAADEALAAAGAVDDAVAAGEQLPSALAGVPLALKDVFTTIDMPTTCGSKILEGWR
                     SPYDATVTARLRAAGIPILGKTNMDEFAMGSSTENSAYGPTRNPWNLDRVPGGSGGGS
                     AAALAAFQAPLAIGSDTGGSIRQPAALTATVGVKPTYGTVSRYGLVACASSLDQGGPC
                     ARTVLDTALLHQVIAGHDRRDSTSLDAEVPDVVGAAKAGAAGDLRGVRIGVVRQLHSG
                     EGYQPGVLASFQAAVEQLTDLGAEVSEVDCPHFEYALPAYYLILPSEVSSNLARFDAM
                     RYGLRVGDDGSHSAEEVMALTRAAGFGPEVKRRIMIGAYALSAGYYDAYYNQAQKVRT
                     LIARDLDEAYKSVDVLVSPATPTTAFRLGEKVDDPLAMYLFDLCTLPLNLAGHCGMSV
                     PSGLSPDDNLPVGLQIMAPALADDRLYRVGAAYEAARGPLPSAI"
     gene            complement(5228..5539)
                     /gene="gatC"
                     /locus_tag="VFI55_13370"
     CDS             complement(5228..5539)
                     /gene="gatC"
                     /locus_tag="VFI55_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET7075056.1"
                     /translation="MSQISRDEVAHLARLTRLALTDAELDSFAGQLDAILTHVSQVQA
                     VDVTGVEATDNPLKDVFKGVDVTRPDETAPCLTQQQALAEAPEAVDGRFAVPQILGDS
                     E"
     gene            5681..6337
                     /locus_tag="VFI55_13375"
     CDS             5681..6337
                     /locus_tag="VFI55_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HET7075057.1"
                     /translation="MPSYLLRIELVDRPGSLGALAVALGSVGADILSLDVVDRSGGYA
                     IDDMVIELPPGAMPDMLITAAESLRGVRVDSVRPHTGLLEAHRELELLDHVAAATDNT
                     TRLQVLVDEAPKVLRVGWCTVLRNSQGELQRLAGSPGTPETKADSAPWLPIERAVTLD
                     SSAEWVPQAWRDMDTTMAAAPLGDPHTAVVLGRPGPQFRPSEVARLGYLAGIVATMLR
                     "
     gene            complement(6348..8432)
                     /gene="ligA"
                     /locus_tag="VFI55_13380"
     CDS             complement(6348..8432)
                     /gene="ligA"
                     /locus_tag="VFI55_13380"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET7075058.1"
                     /translation="MTSAEADPVAPDVRRQWRELADEVREHQFRYYVRDAPIISDAEF
                     DRLLRRLQALEEQYPELRTPDSPTQLVGGAGFATDFTSAEHLERMLSLDNVFSAEEFD
                     VWAARIRAEVGSDVPYLCELKIDGVALALVYRDGRLVRAATRGDGRTGEDVTLNARTI
                     DDVPERLTAAEGYPVPDVLEVRGEVFFRLADFEALNAALVEDGKAPFANPRNSAAGSL
                     RQKDPAVTARRKLRMTCHGVGHTEGFRPATLHEAYLALGAWGLPVSDHTTRVEDVAGA
                     LERITYWGEHRHDVDHEIDGVVVKVDDVALQRRLGSTSRAPRWAIAYKYPPQEVQTKL
                     LDIRVNVGRTGRVTPFAFMTPVKVSGSTVGLATLHNAAEVKRKGVLIGDTVVIRKAGD
                     VIPEVLGPIVDLRDGSEREFVMPTTCPECGTELAPAKEGDADIRCPNARSCPAQLRER
                     VFHVAGRGAFDIEALGYEAAIALLQAGVIADEGDLFSLTEDDLLRTELFTTKSGAVSA
                     NGKRLLANLDKAKSQPLWRVLVALSIRHVGPTAARALATEFGSLDAITSASTEQLAAV
                     EGVGPTIAAAVTEWFTVDWHRAIVDKWQAAGVRMADERDSSVPRTLEGLTIVVTGSLP
                     GFSRDDAKEAIVARGGKAAGSVSKKTAYVVAGDSPGSKYDKAVELGVPILDENGFRRL
                     LAGDPPAAADPG"
     gene            complement(8656..8991)
                     /locus_tag="VFI55_13385"
     CDS             complement(8656..8991)
                     /locus_tag="VFI55_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7075059.1"
                     /translation="MSALKAEAAPLYPFFSALGDANRLRIIVRLCDLGPSSTSQVTQV
                     IPVTRQAASKHLQLLESAGLVTSTRRGRERVWTVRTEPLGQASDYLTQLSRRWDAAVD
                     RLRAFVEEE"
     gene            complement(8988..9479)
                     /locus_tag="VFI55_13390"
     CDS             complement(8988..9479)
                     /locus_tag="VFI55_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7075060.1"
                     /translation="MSTDRIEKQALLRAPLDRVWRAITDAEEFGRWFGVRFDGPFVEG
                     ASIGAAIAPTTVDDEVAERQEPHAGMKSTWHIVAIEPKRRFAYRWHPFAVDANVDYER
                     EPTTLVEFTLAEASDGVLLTITESGFDAIPAARRASSFEANSEGWAIQTDLVRRYLEG
                     TLV"
     gene            complement(9544..10572)
                     /locus_tag="VFI55_13395"
     CDS             complement(9544..10572)
                     /locus_tag="VFI55_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HET7075061.1"
                     /translation="MNVFANVFAKASGVGSWPGTAARGAAEVVVGELAGALAHIVELP
                     ARGVGADLLGRAGALLIDVAIDTVPRGYRIAARPGAVTRRAVSLLDEDMDALEEAWEI
                     AGLRGAGRVVKVQAPGPITLAAGLELANGHRAITDAGAVRDLAASLAEGVAAHRAALT
                     RRLDTPVVVQFDEPSLPAALGGRLTGVTALSPVAALDEAVALALLDACVATVGADVLL
                     HSCAPKLPWDLLQRSAISAVSVDVGTLRAADLDGVAAFVESGRAVMLGVVAATAPERR
                     PSAEEVAAAVVAVTDRLGFARSALRDRIGVTPACGLAGATASWARAAIGLARKAAEAF
                     AEDPDAIQ"
     gene            10658..11302
                     /locus_tag="VFI55_13400"
     CDS             10658..11302
                     /locus_tag="VFI55_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HET7075062.1"
                     /translation="MSQSARFTRPLLWCGAALLAAACTRVVGGSAVPGFTDYAKGVGG
                     VNVDTVLLDQSRMRAITGAGEHLTIIPSMDGNRPVDIDDLADTTPRECRFIFAETATF
                     GPDIQEFHKTTFQDPPDGALISEGAAAYRDADTARRAFGTLVTTVGNCAGSSAGQVLV
                     GDWNANGTSLHLGPGGCGRDYRVRSVAMLEVTFCGFPQSVSDIVMTNIAAKIPG"
     gene            complement(11299..12375)
                     /gene="mnmA"
                     /locus_tag="VFI55_13405"
     CDS             complement(11299..12375)
                     /gene="mnmA"
                     /locus_tag="VFI55_13405"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET7075063.1"
                     /translation="MKVLAAMSGGVDSSVAAARMVDAGHDVVGVHLALSAAPGTLRTG
                     SRGCCSKEDASDARRVADVLGIPFYVWDFAEKFKSDVIDDFVSSYARGETPNPCVRCN
                     QRIKFSALSAKALALGFDTVVTGHYARLSGGRLRRAVDRDKDQSYVLAVLTAEQLRHA
                     AFPIGDTAKPHIRAEAARRGLAVADKPDSHDICFIPSGNTRAFLGERIGVRHGSVVTA
                     DGAVLATHDGVHGFTIGQRKGLGIGGPGPDGRPRYVTAIDAETATVRVGDAADLDVHA
                     LVGRSPVFTSGVAPSGRIECAVQVRAHGEIAGARAELMGDELVVRLTDPLRGVARGQT
                     LVLYRPDPDGDEVLGSATIAETRG"
     gene            complement(12376..13554)
                     /locus_tag="VFI55_13410"
     CDS             complement(12376..13554)
                     /locus_tag="VFI55_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HET7075064.1"
                     /translation="MVYLDHAATTPMHPAAVEAMTAALGTVGNASSLHTSGRTARRRI
                     EESRELIADRLGARPSEVIFTAGGTESDNLAIKGIYWARHDADPRRRRIVASAVEHHA
                     VLDSVNWLVEHEGAQVTWLPTAVDGSVSAAALREALQSHDDVALVSVMWANNEVGTVM
                     PVTELAAVAAEFGVPMHSDAVQAVGQLRVDFAASGLSAMSVTAHKFGGPPAVGALLLR
                     RDVSCVPLSHGGGQERDIRSGTPDVAGAVGMAAAARIAVDGLESHSARLRALRDRLVD
                     GVLTGIDDVSLNGARDPLRLPGNAHFTFRGCEGDALLMLLDANGIECSTGSACTAGVA
                     QPSHVLIAMGADSASARGSLRLSLGHTSVDADVDAVLRVLPGAVDRARRAALAAAGAS
                     "
     gene            complement(13608..14489)
                     /locus_tag="VFI55_13415"
     CDS             complement(13608..14489)
                     /locus_tag="VFI55_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7075065.1"
                     /translation="MNTPGPVPAVTGHSWLPHATCDISCVGLDDAARSRRWLVVLRVG
                     LCVILALLLAPGVPLLVMPLPGRSRVQRAYCRLVLRCFSVRVTVSGNPIRNLRGVLVV
                     SPHMSWLDAFAICSVLPGSVVARADMFTGAATGVLGRFMNIIPIERASLRRLPGVVDT
                     VARRLRAGRTVVCFPEGTTWCGVACGSFYPAMFQAAIDAGRPVQPLRLTYHHVDGSVS
                     TAPAFVRDDELMRSICRLLKVRRTVASVRVEALQLPGTDRRDLARRCQSAVLVGTPEH
                     LGRHSEPQPGHHHVLVA"
     gene            complement(14486..15343)
                     /locus_tag="VFI55_13420"
     CDS             complement(14486..15343)
                     /locus_tag="VFI55_13420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HET7075066.1"
                     /translation="MSIASVLIPSEKPDGAATGAPGPRYSLLLSTDPGMIEAAQRLRY
                     DVFTSTPGFALPAAGAGQVDIDRFDEYCDHLLVRDDDTGELVGCYRMLAPAGAIAAGG
                     LYTETEFDVHAFDPLRPSLVEMGRAVVRDGHRNGGVVLLMWAGILAYLDRYGYDYVSG
                     CVSVPIADESDGPEVLAPGSQLRGVRDFVLSRHAAPLQYRVYPYRPVRVDGVAIDDIA
                     PPLRPSVPPLMRGYLRLGAQVCGEPAHDPDFGVGDFCILLDKRRADTRYLKRLRSVSA
                     ASEMAGGTR"
     gene            complement(15592..16548)
                     /locus_tag="VFI55_13425"
     CDS             complement(15592..16548)
                     /locus_tag="VFI55_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HET7075067.1"
                     /translation="MAEVLVLAEHADGALKKVTAELITAARALGEPSAVVVGAPGTAA
                     PLLDGLTQAGAAKIYVAESDDVDKYLVTPVVDVLAGLAESNGPAAVLLAATADGKEIA
                     GRLAARIGSGLLVDVVEVKQGNKAIHSIFGGAFTVEAQANGDTPVITVRAGAVDAEPA
                     AGAGEQVSVEVPAPAENATKITSREPAVAGDRPELTEATVVVSGGRGVGSAENFSVVE
                     ALADSLGAAVGASRAAVDSGYYPGQFQVGQTGKTVSPQLYIALGISGAIQHRAGMQTS
                     KTIVAVNKDEEAPIFEIADYGVVGDLFKVAPQLTDAIKARKG"
     gene            complement(16577..17368)
                     /locus_tag="VFI55_13430"
     CDS             complement(16577..17368)
                     /locus_tag="VFI55_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HET7075068.1"
                     /translation="MTNIVVLIKQVPDTWSERKLTDGDYTLDREAADAVLDEINERAV
                     EEALQIREREGGEGSVTVLTAGPERAAEAIRKALSMGADKAVHLKDAGLHGSDVVQTG
                     WALARALGAIEGTELVIAGNEATDGTGGAVPAIIAEYLGLPQLTHLRKLSVEGGKVTG
                     ERETDDGVFTLEATLPAVVSVNEKINEPRFPSFKGIMAAKKKEVTVLTLAEIGVEADE
                     VGLENAGSTVLSSTPKPAKSAGEKVTDEGEGGNQVASYLIAQKII"
     gene            17596..18426
                     /locus_tag="VFI55_13435"
     CDS             17596..18426
                     /locus_tag="VFI55_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7075069.1"
                     /translation="MSAFLPDGPQRTPDRLPDQPSPAVDAVLTLTGERTIPDLDIENY
                     WFRRHQVVYQRLALLCADRDVLEAGCGEGYGADLIAAVARRVVAVDYDASAVAHVRGR
                     YPRVEVMQANLAELPLPDASVDVVVNFQVIEHLWDQAQFVAECARVLRPGGVLMVSTP
                     NRITFSPGRDTPINPFHTRELNAGELTELLVDAGFCDVAMRGLFHGSRLQEMDARHGG
                     SIIDAQIARAVADAPWPPGLAADVAAVTIDDFELIEADSYDGPDIDASLDLVAIAVRP
                     "
     gene            18423..20048
                     /locus_tag="VFI55_13440"
     CDS             18423..20048
                     /locus_tag="VFI55_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HET7075070.1"
                     /translation="MSAFERRVPGMFTLVLHTHLPWLAHHGRWPVGEEWLYQSWAAAY
                     LPLLRVLRTLADEDRHSLLTLGVTPVVNAQLDDPYCLDGMHHWLANWRLRATEAAAAA
                     AATPAAAADVRSALRSKSAGHQSSTPKALRASGIRECAEADRALDDFATLWRHGGSPL
                     LRSLLDAGTLELLGGPLAHPFQPLLAPRLREFALREGLADAQQRLGTRKRQSPTGIWA
                     PECAYAPGMEHGYAAAGVTHFMVDGPSLHGDTALGRPVGDTDVVAFGRDLQVSYRVWS
                     PKSGYPGHAAYRDFHTYDHLTGLKPARVTGRNVPSEAKAPYDPERADRAVDVHVADFV
                     DVVRNRLISESERIGRPAHVVAAFDTELFGHWWYEGPTWLQRVLRALPAAGVRVGTLH
                     DAIADGFVGDAVALPPSSWGSGKDWQVWAGDQVSDLVQLNTEVVDTALTTVDKALAQT
                     ASLDGPLPRDRVADQILRETLLTVSSDWPFMVSKDSAADYARYRAHLHAHATREIAGA
                     LASGRRDSARRLAEGWNRADGLFGALDARRLPR"
     gene            20146..>20226
                     /locus_tag="VFI55_13445"
     CDS             20146..>20226
                     /locus_tag="VFI55_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HET7075071.1"
                     /translation="MKILMVSWEYPPVVIGGLGRHVHHLST"
BASE COUNT         3087 a         7203 c         6840 g         3096 t
ORIGIN      
        1 acgacgacaa gacctaactc agccgggcga cgatgcgggc tgtcccggcc cgggcaggct
       61 cagccggcca gctcgcgcgg caccacgaac acgtcgacca gtgcgccgtt gcgccacacc
      121 gtcatctcga tccggcgcgc gatcgcgtcc tcgaccatca gctgctgaat cgcggttgcg
      181 gtgacgatgc gctggccgtc gacgctgacg acgatgtcgc cgcgccgcac gcccgcctcg
      241 gcggccgggc tgcccgcgac gacgctggcg acctgcaggc catgcgcggt gccgagcttt
      301 gccgcgacgg cgggcggtac ggagacatgc gcaccggcga tccccagcca cgcgcgccgc
      361 acccgaccgg tgtgcatcag cgcggcgatg atcttgtgcg tcgaggcgtt gatcggcacc
      421 gccagcccca ggccgacacc ggccaccgcc gtgctcacgc cgaccatccg gccacgactg
      481 tcggcaagta cgccgccgct gtttcccgga ttgagcgcgg catcggtctg gatcacctcg
      541 tcgacgaccc ggccgttgcg ggtgggcagt gacctgccga gaccggacac gatgccggcg
      601 gtcacactgc cggccagccc catcgggttg cccaccgcca ccaccagctg cccgacgacc
      661 aggtcttccg cgcgtcccat gatgaccggc gagggaacgt tgccgcgggc cttcaacacg
      721 gccaggtcgg agagctcgtc gcgaccggtg acatccgcgg cgacgctggt gccgtcgctg
      781 aaggtcgcct cggcggaacc ggccccggcg acgacgtgcg cgctggtcag caggtagccg
      841 tcgggtgtga tgacactggc gctgccactg ccgtcacctc gtcgggtgtg caccgccagg
      901 ctggccacgc tgggcaacac ggcgttcgcc accgccgtga ccacggcgga gtacgcatcc
      961 aaagcttcgt gactgtcttc aggcattacc gggctcagcg tgctgatcgc ttccggtatg
     1021 cccccgttcg ccaggggctg aaccgctagc cctgaccaca ggcggccagc acgagttcgc
     1081 gcacccgcgc cgcgtcggcc tgcccgcggg tggccttcat caccgctccg acgatcgcgc
     1141 cggcggcggc caccttgccg ccgcggatct tttccgcgac atcgggattg gcggccagcg
     1201 cttcgtcgac ggcggcctgc gtcaccgagt cgtcacgcac cagcgccagg ccacgagctg
     1261 tcatcacctg ctcgggttca ccctccccgg ccagcacacc ctccacgact tggcgggcca
     1321 gcttattgga cagcctgcct tcgtcgacca gcgccaccac cgcggcgacc tgggccggcg
     1381 tgatggccag ctcgtccaat ccgatactcg cctcgttggc cttctgcacc agaaagtttc
     1441 cccaccaggc gcgcgcatgc tcgctggacg ccccgtgttt gacggtctcg atcaccaatt
     1501 cgaccgcgcc ggcattgacg agatcccgca tcacctcgtc ggaaataccc cagtcctgct
     1561 gaatccgctt gcggctcaac cacggtagct cggggatggt ctgccgcaat tgctcgaccc
     1621 actcgcggct gggcgcgacg ggctccaagt cgggctcggg gaaatagcgg tagtcctggg
     1681 cggtctcctt ggcgcggcct gggctggtgt acccggcctc atggaagtgc ctggtctcct
     1741 ggatgatccg gccgcccgac gtcagaacgg cgccctggcg ctgcatttcg taacggacgg
     1801 cgacctcgac gcttttgagg gagttgacgt tcttggtctc ggtccgggtg ccgaactcgg
     1861 cggtcccgat gggcttcagc gacacgttgg agtcacaacg catcgagcct tgatccatcc
     1921 ggacgtcgga tacctccaag acgcgcaaca ggtctcgcaa cgccgtcaca taagcgcggg
     1981 cgatctgcgg cgcctgagcc ccggctccca cgatgggctt ggtgacgatt tcgatcagtg
     2041 gcacgccggc acggttgtag tcgatcagcg acgtcgtcgc cccgtggatg cggccggtct
     2101 cgctgcccag gtgggtgagc ttgccggtgt cttcctccat gtgcgcccgc tcgatctcca
     2161 cccgccaggt ggtgccatcc tccagcggtg cttcgaggta gccgttgatg gcgatcggct
     2221 cgtcgtactg cgaaatctgg tagttcttcg gcatgtccgg atagaagtag ttcttccggg
     2281 cgaatcgaca ccagggcacg atctcgcagt tcagcgccaa cccgatccgg atcgcggact
     2341 ggacggcggc ccggttgagc accggcaacg aaccgggcag tcccaggcat accggacaga
     2401 cctgcgtgtt gggttcggcc ccgaaggtgg tggcgcagcc acagaacatt ttggtcacgg
     2461 tggacagctc gacatgcacc tcaagaccga gcaccggatc gaagcgcgcg atgacgtcgt
     2521 cgtaatccag cagctcagcc ttggtagcaa cactcatgcc gtcgatccta atgacgacgc
     2581 cgggagcgtc ggcccgcacg gccgccatct ccggtctgct aaccgaagaa ggcggcggcg
     2641 tcgtcgtagc ggctttcggg caccagcttg agttggcgcg tagcgtcggc gagctgcacc
     2701 cggccgatat cctgcccgcg cagcgacacc atttggccgt actccgccgc gtgcgcggcg
     2761 tcggccgcgg cgacgccgaa ccgggtggcc agcacccggt cgtaggccgt cggggtaccg
     2821 ccccgctgca cgtggcccag cacggtcacg cggacctctt tgttgatccg cctctccacc
     2881 gcgacgccca gctgctgggc gactcccgtg aagcgttcgt gaccgaactc gtcaagtccg
     2941 ccctcgcgca acgtgattga acccgcgatc ggtttggcac cttcggcgac gacgcagatg
     3001 aagtgtgagt ctccgcgttg gaagcgcctt ttgacgagac ggcacacctc ctcaacatcg
     3061 aagggctgtt cggggatcag tgtcatgtgc gcgccggacg ccagcccggc gttgagcgcg
     3121 atccagccgg cgtggcggcc catcacctcc accagcatca cccgctggtg tgattcggcg
     3181 gtgctgtgca gccggtcaat ggcgtcggtg gccacggtca gcgccgtgtc gtggccgaaa
     3241 gtcacgtcgg tgcaatcgat gtcgttgtag atagtcttcg gcacaccgac cacgggaacg
     3301 ttttcctccg acagccaatg cgcggccgtc agggtgccct cgccgccgat cgggatcaac
     3361 acgtcaatgc cgttgtcgtc caaggtctgc ttgatctggt gcagcccggc ccgcagtttg
     3421 tcggggtgca cgcgcgcagt gcccagcatc gttccgccct tgaccagcag ccggtcgttg
     3481 cggtcgtcgt tcttcagctg catgcgccgg ttctccagca aaccgcgcca cccgtcctgg
     3541 aatccgacca ccgaagagcc gtagcgcgag tcgcaggtgc gcaccaccgc ccggataacc
     3601 gcgttgagcc ccgggcagtc gccacctccc gtgagaattc cgatccgcat gcccctcatc
     3661 ttgccccgtg gctcagttcc tggcgcgagc agacgcagaa tcgccctcaa cggcgggttc
     3721 tggtgcgatt ctgtgtctgc tcggcgtcag atggcggacg gcaacggccc gcgcgcggcc
     3781 tcgtaggcgg cgcccacccg gtaaaggcgg tcgtcggcca acgcgggcgc catgatctgc
     3841 aggccgacgg gcagattgtc atccggcgac agccccgacg gtaccgacat gccgcagtga
     3901 ccggccaggt tcagcggcag cgtgcacagg tcgaacaggt acatggccaa cgggtcgtcg
     3961 accttctcgc ccaaccggaa cgcggtcgtg ggtgtcgcgg gcgacaccag cacatcgacg
     4021 gacttgtagg cctcgtcgag atcgcgggcg atcagtgtgc gcaccttctg cgcttggttg
     4081 tagtaggcgt cgtagtagcc agccgacagc gcgtaggcgc caatcatgat gcggcgcttg
     4141 acctctggcc cgaacccggc ggcccgggtc aacgccatca cttcctcggc gctgtggctg
     4201 ccgtcgtcgc cgacccgcag cccgtagcgc atcgcgtcga agcgcgccag gttgctcgac
     4261 acctccgacg gcaggatcag gtagtaggcg ggcagcgcgt attcgaagtg cgggcagtcg
     4321 acttcgctga cctcggcacc caagtcggtc aactgctcga ccgcggcttg gaacgacgcc
     4381 agcacccccg gctggtagcc ctcgccgctg tggagctgac gcaccacccc gatgcgcact
     4441 ccgcgcagat caccggccgc gccggctttg gcggcgccga cgacatcggg cacctcggcg
     4501 tccagggagg tggagtcgcg gcggtcatgc ccggcgatca cctgatgcag cagcgcggtg
     4561 tccagcacgg tgcgcgcgca cggcccaccc tgatccaacg acgacgcgca ggccaccagc
     4621 ccgtagcggg acaccgtgcc gtaggtcggt ttgacaccga cggttgcggt cagcgcggcc
     4681 ggctgacgga tggagccccc ggtgtcggat ccgatggcca gcggcgcctg gaacgcggcc
     4741 agtgccgccg cactgccacc gccggagccg ccgggcaccc ggtcgaggtt ccacgggttg
     4801 cgggtcgggc cgtaggcgga attctccgtc gaggagccca tcgcgaactc gtccatgttg
     4861 gtcttgccca ggatcgggat gccggcggcc cgcaaccgcg cggtgacggt cgcgtcgtag
     4921 ggggaacgcc agccctcgag gatcttggat ccgcaggtgg tgggcatgtc gatggtggtg
     4981 aacacgtcct tgagcgccag cggcacgccg gccagcgccg acggcagctg ctcgccggcg
     5041 gcgaccgcgt cgtcgacggc gcccgcggcc gccagcgcct cgtcggccgc aacgtggagg
     5101 aacgcgcggt accgggcgtc ggtcgcctcg atctggtcca ggcaggcctg ggtgatttcg
     5161 gtggacgaca gctccttggc ggcgatgctg gcagccagcg tcgcggcgtc ggaccgaacg
     5221 atgtcgctca ctcgctgtcc cccaggatct gtgggaccgc gaagcggcca tcgacggctt
     5281 cgggcgcctc ggccagcgcc tgctgctggg tgaggcaggg tgctgtctcg tccgggcggg
     5341 tgacatcgac gcctttaaag acgtccttga gcgggttgtc ggtcgcttca acgccggtga
     5401 cgtcgaccgc ctggacctgg ctgacgtggg tcaggatggc gtcgagctgg ccggcgaagc
     5461 tgtccagctc ggcatcggtc aacgccagcc gggtcagccg cgccagatgc gccacctcgt
     5521 cgcgggagat ctgggacacg acaggaaagc ctaaccgggc tccggccgcg aacgtgaggc
     5581 cggccgcacg ctgggcggcg aacgtgaagc tggccgcccg ctcggcatgc accgtgcagc
     5641 cgaaatgccg tgcacgcatc cctgtgtaac agtgttggcc gtgccgtcgt atctgttgcg
     5701 catcgagctg gtcgaccgcc cgggtagcct gggcgcgctg gccgtcgcgc tgggttcggt
     5761 gggcgccgac atcttgtcgc tcgacgtggt ggaccgcagc gggggttacg cgatcgacga
     5821 catggtcatc gaactgccgc cgggggcgat gcccgacatg ctgatcaccg ccgccgagtc
     5881 gctccgcggt gtccgggtgg acagcgtccg tccacatacc ggcctgctgg aagcgcatcg
     5941 tgagctggag cttctcgacc atgtagccgc ggccaccgat aacacaacca ggctgcaggt
     6001 tctggtcgac gaggcaccca aggtgctgcg cgtcggctgg tgcacggtgt tgcgcaactc
     6061 acagggagaa ctgcaacgcc tcgccggcag cccgggcacg ccggagacca aggccgattc
     6121 cgccccgtgg ctgccgatcg agcgtgccgt aacgctggac agttccgccg aatgggtgcc
     6181 tcaggcgtgg cgcgacatgg acaccacgat ggccgcggcg ccgttgggcg atccgcacac
     6241 ggcggtggtg ctcggccgtc cgggcccaca attccggcct tcggaggtcg cccggctggg
     6301 ctacctcgcc ggaatcgtgg ccaccatgct gcgctgaccg cggcccgcta cccggggtcc
     6361 gccgccgcgg ggggatcgcc cgcgagcaac cggcgaaacc cgttctcgtc gaggatcggc
     6421 acccccagct cgacggcctt gtcgtatttg gagcccggcg agtcgccggc gacaacgtag
     6481 gcggtcttct tcgacaccga gcccgcggcc ttgccgccgc gcgccacgat cgcctccttg
     6541 gcgtcgtcgc gggaaaaccc cggcagcgaa ccggtgacca cgatggtcag gccctctaac
     6601 gtgcgcggca cgctggagtc gcgttcgtcg gccatccgca ccccggcggc ctgccatttg
     6661 tcgacgatcg cgcggtgcca gtccacagtg aaccattcgg tgaccgcggc ggcgatggtc
     6721 gggcccacgc cctcgacggc ggccagctgc tcggtggacg ccgaggtgat cgcgtccagg
     6781 ctgccgaatt cggtggccag cgcgcgggcg gccgtcgggc cgacatggcg gatcgacaac
     6841 gccaccaaca cccgccacag cggctgcgac ttggctttgt cgaggttggc cagcagccgc
     6901 ttgccgttgg ccgacaccgc gccgctcttg gtggtgaaca gctcggtgcg caacaggtca
     6961 tcctcggtga gggagaacag gtctccctcg tcggcgatca ccccggcctg cagcagcgcg
     7021 atggcggcct cgtaacccaa cgcctcgatg tcgaacgcgc cccggccggc gacgtgaaac
     7081 acccgctccc gcaactgcgc cgggcaggac cgggcattgg gacagcggat gtcggcgtcg
     7141 ccctccttcg ccggagccag ctcagtgccg cattcgggac acgttgtggg catgacgaat
     7201 tcgcgttccg agccgtcgcg caggtcgacg atgggaccca atacctcggg aatcacgtcg
     7261 ccggccttgc ggatcaccac ggtgtcgccg atcagtaccc ccttgcgctt gacctcggcg
     7321 gcgttgtgca gggtggccag ccccaccgtc gacccggaga ccttcaccgg tgtcatgaac
     7381 gcgaacggcg tgacgcgccc ggtgcggccg acgttcaccc ggatgtccag cagcttggtc
     7441 tgcacctctt ggggcgggta tttgtaggcg atcgcccacc gcggcgcccg cgacgtggac
     7501 ccgagcctgc gttgcaacgc cacatcgtcg accttgacga cgacaccatc gatttcgtgg
     7561 tcgacgtcgt ggcgatgctc gccccagtac gtgatgcgct cgagcgcgcc ggcgacgtct
     7621 tcgacacggg tggtgtggtc ggagaccggc aggccccagg cgcccagcgc caggtaggcc
     7681 tcgtgcaggg tggcgggccg aaatccttcc gtgtgtccca ctccgtggca ggtcatccgc
     7741 agcttgcggc gtgcggtgac cgccggatcc ttctgccgca gcgatcccgc ggcgctgttg
     7801 cgcgggttgg cgaacggcgc cttgccgtct tcgaccagcg cggcgttcag cgcttcgaag
     7861 tcggcgagcc ggaagaacac ctcaccgcgc acctcgagaa catcgggcac gggatagccc
     7921 tcggcggccg tcaatctttc ggggacgtcg tcgatggtgc gcgcgttcag cgtgacgtcc
     7981 tcgccggtgc ggccgtcgcc gcgggtcgcg gcccggacca gacgcccgtc gcggtacacc
     8041 agagccagcg cgacgccgtc gattttgagc tcacagaggt acggcacgtc gctgccgacc
     8101 tcggcgcgaa tacgcgccgc ccacacatcg aactcctcgg cgctgaacac gttgtcgagg
     8161 gacagcatcc gctccaggtg ctcggcggag gtgaaatcgg tggcgaagcc ggccccgccg
     8221 acgagttgag tcggcgaatc gggcgtgcgc agctcgggat actgctcctc gagcgcttgg
     8281 agccgacgca gcagccggtc gaactccgcg tcggagatga tcggcgcgtc gcgcacgtaa
     8341 tagcggaact ggtgttcgcg cacttcgtcg gcgagctcgc gccactgccg ccgaacgtcg
     8401 ggcgcaaccg ggtcagcttc tgctgaagtc accttcgcag gctatctaag ggcgccgacg
     8461 ccggccgact gcgacgcccc agtcacatct gccacatctg cctctcgtgg acccggtgca
     8521 cattttgccc gcctcagagc gacaattcgc ggggctgcgg ccgaccgagt cggttgtcgc
     8581 tctgaggcgg gcacaaaggc acatcctctg ggagccggga ctgctgtggc cgacgttgcg
     8641 gatgtggcgg ccgactcact cctcttccac gaatgcccgc aaccggtcca ccgcagcgtc
     8701 ccatcggcgc gacaactggg tgaggtagtc gctcgcctgg cccagcggct cggtccgcac
     8761 agtccagacg cgttcgcgac cacgacgggt gctggtgacc aggccggccg actccagcag
     8821 ctgcaggtgc ttgctggccg cctgccgcgt gaccggaatg acttgggtga cttgcgatgt
     8881 cgagctcggg ccgaggtcgc agagccgcac gatgatgcgc agccgattcg cgtcccccag
     8941 cgcgctgaag aaggggtaga gcggcgcggc ctcagccttg agggcgctca tacgagggtg
     9001 ccctccaaat accggcgcac cagatcggtc tggatggccc agccctcgga gttcgcctcg
     9061 aacgaggagg cgcggcgcgc cgcggggatg gcgtcgaagc cggactcggt gatggtgagc
     9121 agcacgccgt cggaagcctc cgcgagcgtg aactcgacca gtgtggtcgg ctcccgctcg
     9181 tagtcgacgt tcgcgtcgac ggcgaagggg tgccagcggt aggcgaatcg ccgcttcggc
     9241 tcgatcgcga cgatgtgcca ggtgctcttc atccccgcgt gcggctcctg acgctcggcg
     9301 acctcgtcgt cgacggtggt cggggcgatg gccgcgccga tcgaggcgcc ctcgacgaac
     9361 ggtccgtcga agcgcacacc gaaccagcgg ccgaactctt cggcgtcggt gatggcccgc
     9421 catacccggt ccagcggggc ccgcaacagt gcttgctttt cgattcgatc agtactcata
     9481 tgcaacctcc tggttgcctt tcagtccacc acatccgcca gcgaaacgca accaaccggt
     9541 tgcctattgg atggcgtccg ggtcctccgc gaacgcctcc gcggccttgc gcgccagccc
     9601 gatggcggcg cgggcccacg acgccgtcgc ccccgccaga ccgcacgccg gggtgacgcc
     9661 gatgcgatcg cgcagcgccg agcgggcgaa gccgagccgg tcggtcaccg cgaccaccgc
     9721 ggcggccacc tcttccgccg agggccgccg ctccggggcg gtcgccgcga ccacgccgag
     9781 catgacggcg cgacccgatt cgacgaacgc ggcgacaccg tcgagatccg ccgcccgcag
     9841 ggtgcccaca tccaccgaaa ccgcactaat cgcactgcgc tgcaatagat cccacggcag
     9901 cttcggcgcg cagctgtgca gcagcacgtc ggcgcccacc gtcgcgacgc aggcgtcgag
     9961 cagcgccagc gccaccgcct cgtcgagcgc ggcgaccgga ctcagcgcgg tcacgccggt
    10021 cagccgtccg cccaatgccg ccggcagcga cggctcgtcg aactgcacca ccaccggcgt
    10081 gtcgagtcgc cgggtcagcg cggcgcggtg cgcggcgaca ccttcggcca gcgaggccgc
    10141 caggtcgcgc accgccccgg catcggtgat cgcccggtgg ccgttggcca gctcgagccc
    10201 ggcggccagc gtgatcggcc ccggggcctg gaccttcacc acgcggccgg cgccgcgcag
    10261 cccggcgatc tcccaggcct cttccaatgc gtccatgtcc tcgtcgagca ggctgacggc
    10321 gcggcgcgtc accgcgccgg gccgggcggc gatgcggtag ccgcggggca ccgtgtcgat
    10381 ggccacatcg atcagcaacg caccggcgcg cccgagcaga tcggcgccca ctccccgggc
    10441 gggcagctcg acgatgtggg ccagcgcgcc cgccaactcg ccgacgacaa cctcggcggc
    10501 gccccgcgca gcggtgccgg gccacgatcc gacgccgctg gccttcgcga agacattggc
    10561 gaaaacattc atccggcaac cgtattcgac gtcgtgtcgc acaccggtcg ccaggggtag
    10621 gtgccgcgag taggttctac caaaagggga gcaaacggtg tcgcagtcgg cgagattcac
    10681 ccggccgttg ctgtggtgtg gcgcggcgct gctggcggcg gcgtgtaccc gggtagtggg
    10741 cggctcggcc gtcccggggt tcaccgacta cgccaagggc gtcgggggcg tcaacgtcga
    10801 caccgtgctg ttggaccagt cgcggatgcg ggcgatcacc ggcgccggcg agcacctgac
    10861 gatcattccc tcgatggacg gcaaccgccc ggtggacatc gacgacctcg ccgacaccac
    10921 gccgcgggaa tgccgattca tcttcgccga gacagcgacg ttcgggcccg atatccaaga
    10981 gttccacaag accaccttcc aggacccgcc cgacggggcc ctgatctccg agggcgctgc
    11041 ggcctaccga gacgccgaca ccgcgcggcg cgccttcggc accctggtca ccaccgtcgg
    11101 gaactgtgcg ggcagctcgg ccggccaggt gctggtcggc gactggaacg cgaacggcac
    11161 gtcgctgcac ctgggtccgg gcggctgcgg ccgcgactac cgggtgcggt cggtggccat
    11221 gctggaagtc actttctgcg ggtttccgca gtcggtgtcc gacatcgtga tgacgaacat
    11281 cgccgccaaa atcccgggtt agccgcgcgt ctcggcgatg gtggcgctgc cgagcacctc
    11341 gtcgccgtcc gggtccgggc ggtagagcac cagcgtctgg ccacgcgcca ccccgcgcag
    11401 tggatcggtc aatcgcacca caagctcgtc gcccatcaat tcggctctgg caccggctat
    11461 ttcgccgtgc gcccgcacct gcaccgcgca ctcgatccga cccgacggcg cgacacccga
    11521 ggtgaacacc ggggaccgcc cgaccagcgc atgcacgtcc agatcggcgg cgtcgcccac
    11581 ccgcacagtc gcggtctcgg catcgatcgc cgtcacgtag cgcgggcggc cgtcgggccc
    11641 cggcccgccg atgcccaggc ccttgcgttg cccgatggtg aaaccgtgca ccccgtcatg
    11701 ggtggccagc acagcgccgt ccgcggtgac cacgctgccg tggcgcaccc cgatccgctc
    11761 gcccaggaaa gcgcgggtgt tcccggacgg gatgaagcag atgtcgtgac tgtccggctt
    11821 gtcggcgacg gcaaggccac gacgggcggc ctcggcgcgg atgtggggct tcgccgtatc
    11881 gccgatggga aacgccgcgt ggcgcaactg ttctgccgtc agcacggcca gcacgtaaga
    11941 ctgatccttg tcgcgatcga cggcgcggcg cagccgcccg ccggacagcc gggcgtagtg
    12001 gccggtgacc accgtgtcga agcccagcgc aagggctttc gccgacagcg ccgagaactt
    12061 gatgcgctga ttgcaccgga cgcaggggtt cggtgtttcc ccgcgcgcgt aggacgacac
    12121 gaaatcgtcg atcacgtcgg atttgaactt ctccgcgaaa tcccaaacat agaacgggat
    12181 tccgagcaca tcggcgacac ggcgcgcatc cgaggcgtct tctttggaac agcagccccg
    12241 cgagccggtg cgcagcgtgc cgggcgcggc ggacagcgcc aagtgcacac cgaccacgtc
    12301 gtgtccggcg tcgaccatcc gggcggcggc gaccgacgag tcgacgccgc cactcatcgc
    12361 agcgaggacc ttcacctacg aagcccccgc agcggccagg gcggcccgtc gagcgcggtc
    12421 cacggccccg ggcagcaccc gcaacaccgc gtccacgtcg gcgtcaacac tggtgtgccc
    12481 caaagaaagt cgcaacgatc cgcgggcgct ggccgagtcg gcgcccatcg cgatcagcac
    12541 gtgcgacggc tgcgcgacac cggcggtgca ggccgacccg gtcgagcatt cgattccgtt
    12601 ggcgtccagc aacatcaaca gcgcgtcgcc ctcacagcca cggaacgtga agtgcgcgtt
    12661 gcccggaagt cgcagcgggt cgcgcgcgcc gttgaggctg acgtcgtcga tgccggtcag
    12721 caccccgtcg accaggcgat cccgcagggc gcgcagccgg gcactgtggg actcgagtcc
    12781 gtccaccgcg atgcgggcgg ccgccgccat tccgacggcg cccgcgacat cgggcgtacc
    12841 ggagcggatg tcgcgctcct gcccgccgcc gtgggacagc ggcacgcagc tgacgtcgcg
    12901 gcgcagcagc agcgcgccca ccgccggcgg cccaccgaat ttgtgcgcgg tgacgctcat
    12961 cgccgacagc ccgctggcgg cgaagtcgac gcgcagctgc cccaccgcct ggacggcgtc
    13021 gctgtgcatc ggaaccccga attcggcggc gacggccgcg agttcggtca ccggcatgac
    13081 ggtgccgacc tcgttgttgg cccacatcac cgataccagg gcaacatcgt cgtggctttg
    13141 cagcgcctca cgcagcgcgg ccgccgacac cgagccgtcg acagcggtgg gcagccaggt
    13201 cacctgggcg ccttcgtgtt cgacgagcca gttcaccgag tccagcacgg cgtggtgttc
    13261 gaccgcactg gcgacgatgc gccggcggcg cgggtcggcg tcgtgacggg cccaatagat
    13321 gcccttgatg gccaggttgt cgctctcggt gccgcctgcg gtgaagatca cctccgacgg
    13381 ccgcgcaccc agccggtctg cgatcagctc ccgcgattcc tcgatccgcc gccgcgccgt
    13441 ccgcccgctg gtgtgcagcg aggaagcgtt gccaacggtg ccgagcgcgg ccgtcatcgc
    13501 ctcgacggcg gcggggtgca tcggggtggt ggcggcgtga tccaggtaga ccatgacgcg
    13561 ccccacgata cctgcagcgc cctggcaact gctcgcgagc gagcgggtca ggccaccagc
    13621 acgtggtggt gacccggttg cggctcggaa tgccggccca aatgctccgg agtccccacc
    13681 agcacggcgg actggcaacg gcgggcaagg tcgcgccgat cggtgccggg caactgcagg
    13741 gcttcgaccc gcaccgacgc caccgtgcgg cgcaccttga gcagacggca gatcgaccgc
    13801 atcaattcgt cgtcgcgaac gaacgccggg gccgtggaga cgctgccatc gacgtgatgg
    13861 taggtcagcc gaagtggctg taccgggcgc ccggcatcga tcgcggcctg gaacatcgcc
    13921 ggatagaagg agccgcacgc cacgccgcac caggtggtgc cctccggaaa gcacaccacc
    13981 gtccggccgg cgcggagccg gcgcgcgacg gtgtccacca cgccgggcag ccgccgcaga
    14041 ctggcccgct cgatcgggat gatgttcata aagcgaccca agacgccggt ggccgccccg
    14101 gtgaacatgt cggctcgcgc cacgaccgac ccgggcagca ccgaacagat ggcgaaggcg
    14161 tccaaccacg acatgtgggg gctgaccacc aggactccgc gcaggttgcg aatcgggttg
    14221 cccgacaccg tgacccggac actgaagcag cgcaggacca atcggcaata ggcgcgttgc
    14281 acccgcgacc ggccgggcag cggcatcact agcaatggca ctcccggcgc cagcaacagc
    14341 gccagtatca cgcacagccc cacccgcagc accaccagcc accgccgtga ccgcgcggcg
    14401 tcgtcgagac ccacacagct gatgtcgcac gtcgcgtggg gcaaccagga gtggccggtg
    14461 actgcgggaa cggggcccgg ggtgttcatc gcgtgccgcc tgccatttca gaagccgccg
    14521 acaccgaccg cagtctcttg agatatcggg tatcggctcg tcgtttgtcc agcaggatgc
    14581 agaagtcgcc cactccgaag tccgggtcgt gcgcgggctc gccgcagacc tgcgcgccca
    14641 gccgcaggta gccgcgcatg agcggcggga ccgagggacg cagcggcggc gcaatgtcgt
    14701 cgatggccac cccgtccacg cgcaccggcc ggtagggata cacccggtac tgcagcggcg
    14761 cggcatgacg gctcaggacg aagtcgcgca ccccgcgcag ctggctcccc ggcgccagaa
    14821 cctccgggcc gtcggattcg tcggcgatcg gtaccgacac gcacccgctc acgtagtcgt
    14881 agccgtaacg gtccaggtag gccaggatgc cggcccacat cagcagcacg acgccgccgt
    14941 tgcggtgacc gtcccgtacc accgcgcgtc ccatctccac caacgagggc cgcagcgggt
    15001 cgaaggcgtg gacgtcgaat tccgtctcgg tgtagaggcc cccggccgcg atcgcacccg
    15061 cgggggccag catccggtaa caaccgacca actcgccggt gtcgtcgtca cgaaccagca
    15121 ggtggtcgca gtactcgtcg aacctgtcga tgtcgacttg tccggcaccg gccgccggca
    15181 gggcgaaccc gggtgtgctg gtgaacacgt cgtagcggag ccgctgcgcc gcctcgatca
    15241 tgccggggtc ggtcgacaac aacagcgaat agcgcggtcc gggtgcaccg gtcgccgcgc
    15301 cgtctggctt ctcgctgggt atcaggacag aagcaatgct catgggaaac aacgtggcgt
    15361 agtcgatgag ctaatcggca tcggcgctgt gacgtgtcgg tgcacgtcag atgacgaaat
    15421 gaggctgacc gggcggggcc gagccggctg tgcgacgcct catccgtcac ttgagtcacc
    15481 acggcgggtc ggtgtggggc ttttgccgcc ctcacagcga ctgcgcacca tggttccgcg
    15541 cctgccacca gggttttccc acgtttgcgg gcgctgaggc ggcgcgtgtt gttaaccctt
    15601 gcgggccttg atcgcgtcgg tcagctgggg agcgaccttg aacaggtcgc ccaccacgcc
    15661 gtagtcggcg atctcgaaga tgggcgcctc ttcgtccttg ttgaccgcga cgatggtctt
    15721 ggatgtctgc atgcccgcgc ggtgctggat cgcgccggag atacccagcg cgatgtagag
    15781 ctgcggcgac accgtcttgc cggtctgacc cacctggaac tggcccgggt agtagccgga
    15841 gtcgaccgcg gcgcgagagg cccccacggc agcgcccagc gaatcggcca aagcctcgac
    15901 cacgctgaag ttctccgcgc tgccgacgcc gcggccaccg gacaccacga cggtggcctc
    15961 ggtcagctct ggacgatcac cggcgaccgc gggctcacgc gaggtgatct tggtcgcgtt
    16021 ctcggcggga gccggcactt ccacgctgac ctgctcaccg gcaccggcgg cgggttcggc
    16081 gtcgacggca ccggcgcgca cggtgatcac cggggtgtcg ccgttggcct gcgcctcgac
    16141 ggtgaacgca ccaccgaaga tggagtggat ggccttgttg ccctgcttga cctcgaccac
    16201 gtcgaccagc aggccggatc cgatccgggc cgccagccgg ccggcgatct ccttgccgtc
    16261 cgcggttgcg gccagcagca cggcggcggg cccgttggat tcggccaacc cggccagcac
    16321 gtcgaccacc ggtgtgacca ggtatttgtc gacgtcgtcg gactcggcga cgtagatctt
    16381 ggcggcgccg gcctgcgtca gcccgtcgag cagcggcgcg gccgtccccg gcgcgccgac
    16441 cacgacggcg gacggctcgc ccagcgcgcg ggcggcggtg atcaattcgg cggtgacctt
    16501 cttcaacgcg ccatcggcgt gctcggcgag caccagtact tcagccatgg tttatcgctc
    16561 ttctcgtgag tgctcgtcag atgatcttct gggcgatcag gtacgaagcg acctggttgc
    16621 cgccctcgcc ctcgtcggtg accttctcgc ccgcggactt agccggcttc ggcgtcgacg
    16681 acagcacggt cgacccggca ttttccagcc cgacctcgtc ggcctcgacc ccgatctcgg
    16741 ccagcgtcag cacggtgacc tctttcttct tggcggccat gatgcctttg aaggacggga
    16801 agcgtggctc gttgatcttc tcgttcacgc tcaccaccgc gggcagcgtg gcctcgaggg
    16861 tgaacacacc atcgtcggtc tcgcgctcac cggtgacttt gccgccctcg accgacagtt
    16921 tgcgcaggtg ggtcagctgc ggcaggccca gatactcggc gatgatggcc ggcacggccc
    16981 cacccgtgcc gtcggtggcc tcgttgcccg caatcaccaa ctcggtaccc tcgatggccc
    17041 ccaacgcgcg cgccagcgcc cagccggtct gcaccacgtc cgagccgtgc agcccggcgt
    17101 ccttgaggtg aacggccttg tcggcgccca tcgacagggc cttgcggatc gcctcggccg
    17161 cgcgctcggg gcccgcggtc agcacggtca ccgatccttc gccgccctcc cgctcccgga
    17221 tttgcagcgc ctcctcgacg gcgcgctcgt tgatctcgtc cagcaccgcg tcggcggcct
    17281 cgcggtccag cgtgtaatcg ccgtcggtga gcttgcgctc cgaccaggtg tctgggacct
    17341 gcttgatcag gaccacgatg ttcgtcatga gagtggtttg tcctcctcga aggagtcccg
    17401 cagcggtcga ctgcgggacc tcggtcacgc gattgttcac cgcacggcat gttaccgacc
    17461 ggtaactttg cgcggtctgc ccccacacca tagcgggtcg tagtgcaggt tctttcgggc
    17521 tgcccacccc tcccgacagg gccgcccaca tgcccggttc gcgaatcgga cacttcgcgt
    17581 tagcctgcct aggtaatgag cgcattcctc cccgacggtc cccagcgcac ccccgaccgc
    17641 ctgcccgacc agccctcgcc ggcggttgac gccgtgttga cgctgacggg cgagcgcacg
    17701 atccccgatc tggacatcga gaactactgg tttcgccgcc accaggtggt ttaccagcgg
    17761 ctcgcgctgc tctgcgccga ccgtgacgtg ctcgaggccg gctgcggcga gggctacggc
    17821 gccgacctga tcgccgctgt cgcacgccgg gtggtcgcgg tggactacga cgcgtccgcg
    17881 gtggcccacg tccgcggccg ctacccccgg gtggaggtca tgcaggcaaa cctcgccgag
    17941 ctgccgctgc ccgacgcctc ggtggacgtt gtggtcaact tccaggtcat cgagcacctg
    18001 tgggatcaag cacagttcgt cgccgaatgc gcccgggtgc tgcgtcccgg cggcgtgctg
    18061 atggtgtcca cacccaaccg gatcaccttc tcacccggcc gcgacacgcc gatcaacccg
    18121 ttccacaccc gggaactcaa cgccggcgag ctgaccgagt tgctggtcga cgcgggattt
    18181 tgtgacgtgg ccatgcgcgg gttgtttcat gggtcgcggc tgcaggagat ggacgcccgg
    18241 cacggcggct ccatcatcga cgcgcagatc gcgcgggcgg tggccgacgc gccgtggccg
    18301 cccgggctgg cggcggatgt cgccgcggtc accatcgacg acttcgagct gatcgaggcg
    18361 gacagctacg acggcccgga catcgacgcc agcctggacc tcgtcgcgat cgcagtgcgg
    18421 ccatgagcgc attcgaacgc cgcgtgcccg ggatgttcac cctggtcctg cacacccatc
    18481 tgccgtggct ggcccaccac ggccgctggc cggtcggcga ggaatggctc taccagtcct
    18541 gggcggcggc ctacctgccg ctgctgcggg tgctgcgcac gctggccgac gaagatcggc
    18601 acagcctgct caccctcggt gtgacgccgg tggtcaacgc gcagctcgat gacccgtact
    18661 gcctcgacgg catgcatcac tggctggcca actggcggct gcgggccact gaagcagccg
    18721 cagcagcagc agctacacca gccgccgcgg ccgatgtgcg gtctgcgctg cgatcgaagt
    18781 cggccggcca ccagtcaagc acaccgaaag cactgcgcgc ctccggaatt cgagaatgcg
    18841 ccgaggcgga ccgggcgctc gacgactttg ccaccctgtg gcggcacggc ggcagcccgc
    18901 tgctgcgcag cctcctcgac gccggcacgc tggagctgct cggcgggccg ctggcccacc
    18961 cgttccagcc gctgctcgcg ccgcggctgc gcgagttcgc gctgcgcgag ggcctggccg
    19021 acgcccaaca gcggctaggg acgcgaaaga ggcaaagccc gacggggatc tgggcgcccg
    19081 aatgcgccta cgccccgggc atggagcacg gctacgccgc cgccggcgtc acgcacttca
    19141 tggtcgacgg cccgtcactg cacggcgaca ccgccctggg ccggccggtc ggtgacaccg
    19201 acgtggtcgc gttcgggcgc gacctgcagg tcagctaccg ggtgtggtcg ccgaaatccg
    19261 gctaccccgg gcacgccgcg taccgcgatt tccacaccta cgaccacctg actgggctca
    19321 agccggccag ggtgaccggc cgcaacgtgc cctcggaggc caaggcgccc tacgatcccg
    19381 agcgcgccga ccgcgcggtc gacgtccacg tcgccgactt cgtcgacgtg gtgcgtaacc
    19441 ggctgatcag cgagtccgaa cgcatcggcc ggccggctca tgtcgtcgcg gccttcgaca
    19501 ccgagctgtt cggccactgg tggtacgaag gcccgacgtg gctgcagcgg gtgctgcggg
    19561 cgttgcccgc ggcgggcgtg cgggtgggca cgctgcacga cgccatcgcc gacgggttcg
    19621 tcggcgacgc cgtcgcgctg ccgcccagct cgtggggttc gggcaaggac tggcaggtgt
    19681 gggccggcga ccaggtgtcc gacctggtcc agctcaacac cgaggtcgtc gacacggcgc
    19741 tgaccacggt cgacaaggcg ctggcacaga cggcctcgct ggacgggccc ctcccccgcg
    19801 accgcgtcgc ggatcaaatc ctgcgcgaga cgctgctcac cgtttccagc gactggccgt
    19861 tcatggtgag caaggactcg gccgccgact acgcccgtta ccgtgcccac ctgcacgcac
    19921 acgccacccg ggagatcgcc ggggcactgg cgtcgggccg gcgcgacagt gcgcgtcgac
    19981 tggccgaggg atggaaccgg gccgacggtc tgttcggcgc tctggacgca cgtaggctgc
    20041 cccggtgagc cggccgtcga cccgcgaaag tgcgtgtttg tacgccgaca cgccgcgtgg
    20101 ggcggcattc tgcgcacgct cgcaaccgga cgacgggccg ggcgcatgaa aatcctcatg
    20161 gtgtcgtggg agtacccgcc ggtggtcatc ggcgggctcg gacggcacgt gcatcacctg
    20221 tcgacc
//
LOCUS       DASSGQ010000303         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1207436,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000303 DASSGQ010000000
VERSION     DASSGQ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207436"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VFI55_13450"
     CDS             complement(<1..647)
                     /locus_tag="VFI55_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075072.1"
                     /translation="MDIVLGVSMAPETVRMVLVEGEGAGGVTVDQADFDVAGDAAADQ
                     VIAAILGTGESAAESGYQLTSSGVTWTDETEAASLRNALAARKIENVVLVSAFVAAAA
                     LAQAVGSATNSARTALLFVEPATATLAVVDTSDGSIIDVHRKPLPAADDAVLAELAAM
                     VAGAESMDARPDGVFLVGSGVDVPSIKPALEAATSLSVTAPEEPETALARGAALAS"
     gene            complement(777..2267)
                     /locus_tag="VFI55_13455"
     CDS             complement(777..2267)
                     /locus_tag="VFI55_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075073.1"
                     /translation="MVGIVLGVSMAPTKVRMVLVEGECADGVTVDQDHFDVDGYVSPS
                     AASSQVISAILGTREGATAGGYQLLSTGVTWTDHVEAALLRDALVAHKIENVMLVSAF
                     MAAAALTQAVGDATNYARTALLFVEPYTATLAVVNTADGSISEVTRRMLADDEDQAVA
                     ELVELVAATEALEVRPEGVVVVGSGVDIPLIKPALEAATCLELSVPDEPETALARGAA
                     LASANAPLFVSSTAALAYALDPGTGEVDPLALAGQFPGLLPGYLPEYHAVPELPADVD
                     KGKANVAYSALPDEDAEAHTVIGLDEAAQRRKSFLLVGSTLAVIFISAVVALELALAI
                     SIRPTVALRPSPNENIVAPAQPAPVPAPQAMPRIAAPAPVAVPHPMNPPAIAPAPVAA
                     PPVPVVPPPVFVPIPRVPVVAPLVPGFGGGGGPFGGEPGPRGPFGDDGPGGRGPFGHG
                     PFGRGPFGGGPFGHGPFGGGHGPFGGLFGGHGGFGGGHGFGGFGHR"
BASE COUNT          410 a          933 c          873 g          379 t
ORIGIN      
        1 gaggccagcg ccgcccctcg cgccaatgcc gtctcgggct cctcgggtgc ggtgaccgaa
       61 agcgacgtcg ccgcctccag tgccggcttg attgacggga cgtctacacc ggagccgacg
      121 aggaacaccc catcggggcg agcgtccatc gattcggcac ccgcgaccat cgcggccaac
      181 tcggccagca ccgcgtcgtc agcggcgggc aggggttttc gatgcacgtc gatgatcgac
      241 ccgtcggagg tgtcgacgac ggccagcgtc gcggtggccg gttccacgaa cagcagcgcg
      301 gtgcgggcgc tgttggtcgc gctgccgacg gcctgcgcca gtgcggccgc cgccacgaac
      361 gccgacacca gcacgacgtt ctcaatcttg cgtgccgcca acgcatttcg cagcgaggcc
      421 gcctcggttt cgtcggtcca ggtcaccccg ctcgacgtca gctggtagcc gctctcggcc
      481 gcgctttccc cggtcccgag aatcgccgcg atcacttggt cggccgcggc atcaccggcg
      541 acgtcaaagt cggcctgatc gacggtcacc ccgccggcgc cctcgccttc gacgagcacc
      601 atgcggaccg tctcgggtgc catcgacacc ccaagcacga tgtccaacgc gctcctccac
      661 gttccccgaa tcggtgcgcg gatttcataa agccgaactt cgcagcaaat tctgggccgc
      721 gaccgaaccc tagtgcgtca ccctagcccg ctggtcggga cgcgcttggt gaaacgctac
      781 cgatgcccga agccgccgaa cccgtgcccg ccaccgaaac cgccgtggcc gccgaacagc
      841 ccaccgaagg gaccatgtcc gccaccgaag ggtccgtgac caaagggtcc gccgccgaag
      901 ggtccgcgac cgaagggccc gtgaccgaag ggtccgcggc cgccggggcc gtcgtcaccg
      961 aaaggcccgc ggggacccgg ctcgcccccg aagggcccgc cgccgccccc gaatcccggc
     1021 acgagcggcg cgaccaccgg gacccgggga atcgggacga aaacgggcgg cggaaccacc
     1081 gggacgggcg gcgccgccac cggggccggg gctatggccg gtgggttcat cggatgcggc
     1141 accgcgaccg gtgcgggcgc cgcgattctc ggcatcgcct gcggcgcagg cacgggcgca
     1201 ggctgggctg gggcgacgat gttttcgttg ggactcggac gcaatgcgac cgtggggcgg
     1261 atgctgatcg ccagcgcgag ctccagcgcc actaccgcgc tgatgaagat caccgccagc
     1321 gtgctgccga ccagcaggaa agatttgcgc cgctgggcgg cctcgtccag gccgatgacg
     1381 gtgtgggcct cagcgtcctc gtccggtagc gcgctgtagg cgacgttggc cttgcccttg
     1441 tcaacgtcgg cgggaagctc tggaacggcg tggtattcgg gcaggtagcc gggtagtagt
     1501 ccgggaaact ggccggccag cgccaacggg tcgacctcgc cggtgccggg gtccagcgcg
     1561 tacgccagcg ccgcggtcga cgatacgaac agcggcgcat tcgccgacgc cagcgccgcg
     1621 cctcgggcga gggccgtttc cggctcgtcg ggtaccgaca gctcaaggca tgtcgcggcc
     1681 tccagcgcgg gcttgatcag cggaatatcg acgcccgagc cgacgacgac gacaccctcc
     1741 gggcgcactt ccagcgcttc ggtggcggcg accagctcga cgagttcggc caccgcctgg
     1801 tcttcgtcgt cggcgagcat ccggcgggtg acttcggaga tcgatccgtc ggcggtgttg
     1861 accacggcca gggtcgccgt gtagggctcg acgaacagca gcgcggtgcg cgcgtagttg
     1921 gtggcgtcgc cgacagcctg cgtgagcgcc gccgcggcca tgaacgccga gaccagcatg
     1981 acgttctcga tcttgtgggc gacgagcgcg tcgcgcagca gcgcggcctc gacgtgatcg
     2041 gtccaggtca ccccggtcga gagcagctga tagccgcccg ctgtagcgcc ttcccgcgtg
     2101 cccaggatgg cggagatcac ctggctggac gccgctgagg gcgacacgta tccgtccacg
     2161 tcgaagtggt cctggtcgac ggtgactccg tcggcgcatt caccttcgac cagcaccatg
     2221 cggaccttcg tcggcgccat cgagacgcca agcacgatgc ccaccaccgc cagagttccc
     2281 cctttggtag tttgctgtgt tgtccgacaa cgctgtcgac actggctatt gaccacggat
     2341 cttattgcca cagcagcgcg cgggcgctct attccgggat tagctgggcg atatgaagtt
     2401 ctggctctcg gaaaccgggc ggcacgcctt ctcggcccgc cacaacactc cgaccctacc
     2461 cgcctcgccg ggggcaaggc acgcgcaagt cgacaagcgt taaccggttt gcaacattgc
     2521 gcaacacgct gccgtcagcc gaggttacct gtgcccaccg ccgccaccgc cgccgtggcc
     2581 gccgccaccg ccgcc
//
LOCUS       DASSGQ010000304         3996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1055012,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000304 DASSGQ010000000
VERSION     DASSGQ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055012"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="VFI55_13460"
     CDS             complement(<1..1137)
                     /locus_tag="VFI55_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7075074.1"
                     /translation="MRTEPVFRNHPIPALAVICLGVFVISVDATIVNVALPTLSRELG
                     ADTAQLQWIVDAYTLVMSGLLLSAGSLSDRYGRRGWLGGGLALFAVTSAVAGQVNSAD
                     ALIAARAAMGVGAAVIFPTTLGLITNIFTDPVPRAKAIGVWAAMVGVGVAVGPISGGW
                     LLEHFRWGSIFMVNIPIAAVTIIGAVLFVPTSRDPAAPRVDVPGLILSAAGITTLVYT
                     VIEAPTWGWTNARTATGFITAALILAGFALWERRSTHPMLDVSVFFNRRFSGGSLAVT
                     AGFLTLFGFIFVITQYFQFIKNYTAFASGVRLLPVAVSIALASVVGPWLVERVGTTAV
                     VAAGLTVFAAGLAWASTADAATPYGQIATQMLLLGGGLGLTTAPA"
     gene            1218..1859
                     /locus_tag="VFI55_13465"
     CDS             1218..1859
                     /locus_tag="VFI55_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7075075.1"
                     /translation="MPPRRNYNQNCPIAYGLDVLGERWTLLILRELVGGPRRYGDLRA
                     ELPGIATNLLAERLKELQDAGLVDRADLPAPIGRTVYTLSDMGWQRVLPILQSIAWFG
                     LDRLDLIEDGPVSPLTGFLAGFVVPFNPLKAAELNATYRVEIDGRRFEFAVTQGSLAA
                     PHGEPTVTVTAGAADLVTARLGPTEARRKAALRRINFGGDRDAVDALRQAFSL"
     gene            1912..3513
                     /gene="guaA"
                     /locus_tag="VFI55_13470"
     CDS             1912..3513
                     /gene="guaA"
                     /locus_tag="VFI55_13470"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET7075076.1"
                     /translation="MAEPADVDVHATSARPVLVVDFGAQYAQLIARRVREARVFSEVI
                     PHTASIEEIKARDPLALVLSGGPASVYAEGAPQLDPAVFDLGVPVFGICYGFQAMAQA
                     LGGTVAHTGTSEYGRTELKVLGGELHSGLPDVQPVWMSHGDAVTAAPEGFEVAASSAG
                     AAVAAFEDRDRRLAGVQYHPEVMHTPHGQQVLSRFLHDFAGLGAQWTPANIAGALIEQ
                     VRAQIGDGYAICGLSGGVDSAVAAALVQRAIGDRLTCVFVDHGLLRAGERAQVQRDFV
                     AATGANLVTVDAAETFLEALSGVTNPEGKRKIIGRQFIRAFEGAVRDIMSDIVNDIVS
                     DNSIDFLVQGTLYPDVVESGGGSGTANIKSHHNVGGLPDDLKFKLVEPLRLLFKDEVR
                     AVGRELGLPEEIVARQPFPGPGLGIRIVGEVTADRLDTLRRADSIAREELTAAGLDNQ
                     IWQCPVVLLADVRSVGVQGDNRTYGHPIVLRPVSSEDAMTADWTRVPYEVLERISTRI
                     TNEVAEVNRVVLDITSKPPGTIEWE"
     gene            3761..>3996
                     /locus_tag="VFI55_13475"
     CDS             3761..>3996
                     /locus_tag="VFI55_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075077.1"
                     /translation="MTTAFASDHRAEQLESLRRQMAGVSEKMSGKVPRSVVTGADDLL
                     PESEARLPLPQWLAESLPVQLPRGTVAVLSGARSL"
BASE COUNT          690 a         1290 c         1399 g          617 t
ORIGIN      
        1 ggcgggcgcc gtggtcaggc cgaggccgcc gccgagcagc agcatctgcg tggcgatctg
       61 accgtagggc gtggccgcgt ccgctgtgga cgcccacgcc agcccggcgg cgaacacggt
      121 gaggccggcg gcgacgacgg cggtggtgcc gactcgctcg accagccaag gacccacgac
      181 gctggccagt gcgatcgaga ccgcaaccgg cagcaagcgc acaccgctgg caaatgcggt
      241 gtagttcttg atgaattgga agtactgggt gatgacgaag atgaagccga acaacgtcag
      301 aaagccggcg gtcaccgcca ggctgccgcc ggagaaccga cggttgaaaa acaccgagac
      361 gtccagcatc ggatgggtgc tgcgccgctc ccacagcgca aatccggcga gaatgagtgc
      421 ggccgtgatg aagccggtag cggtccgcgc gttggtccat ccccaggtgg gcgcttcgat
      481 caccgtgtag accagcgtcg tgatcccggc cgccgacagg atcaagccgg ggacgtcgac
      541 gcgcggcgcg gccggatcgc gcgacgtcgg cacgaacaag acggcaccga tgatggtcac
      601 cgccgcgatc ggaatgttca ccatgaagat cgagccccac cggaagtgct cgagcaacca
      661 gccgccgctg atcggtccga cggccacccc gacgcccacc atggccgccc acaccccgat
      721 cgccttcgcg cggggtaccg ggtcggtgaa gatgttggtg atcagtccca atgtggtcgg
      781 gaagatcacc gccgccccga cgcccatggc ggcgcgcgcc gcgatcagcg catccgccga
      841 attcacctgc cccgcaaccg ccgaagtgac cgcgaacagt gcgagcccgc cgccgagcca
      901 gccgcgcctg ccgtagcggt cgctgaggct gccggccgag agcagcaggc cggacatgac
      961 cagggtgtac gcgtcgacga tccattgcag ttgcgcggtg tcggcgccga gctcgcgcga
     1021 cagggtgggc agcgcgacgt tgacgatggt ggcgtcgacg ctgatgacga agacgccgag
     1081 gcagatcacg gcaagcgcgg ggatcgggtg attccggaac accggttcgg tacgcatgaa
     1141 caagacatta aagtcgatag acaaataaat caagtggtaa cttgaaaaaa atgtctacct
     1201 gttgataggc tggcttggtg ccgccccgca ggaactacaa ccagaactgt ccgatcgcct
     1261 acgggctcga tgtcctgggc gagcgatgga cgttgctgat cctgcgcgag ctggtcggcg
     1321 gaccccgccg ctacggcgac ctccgcgccg agctacccgg cattgcaacc aatctgctcg
     1381 ccgagcgact caaagaactg caggacgccg ggctcgtcga ccgggccgat ctaccggccc
     1441 cgatcgggcg taccgtgtac acgctcagcg acatgggctg gcagcgggtg ctgcccatcc
     1501 tgcagagcat cgcgtggttt ggcctggacc ggctggacct gatcgaggac ggtccggtgt
     1561 cgccattgac ggggttcctc gccggattcg tggtcccctt caacccgctc aaggccgcgg
     1621 agttgaatgc gacgtatcgc gtcgagatcg acggccgccg tttcgagttc gccgtcacgc
     1681 agggcagtct ggcggctccg catggtgaac cgaccgtgac ggtcacggcc ggtgcggcgg
     1741 atctggtcac ggcacggctt gggcccaccg aagccaggcg caaggcggcg ctgcggcgaa
     1801 tcaacttcgg cggtgaccgc gacgccgtcg acgcgcttcg tcaggccttc tcgctctagg
     1861 cgtgcgcctg ctgaacaggg atgacgccgg cagccactag actgactggc cgtggccgaa
     1921 cctgctgatg tcgacgtgca tgcaacgtca gcccgaccgg tgctcgtcgt cgacttcggc
     1981 gcacagtatg cgcagttgat cgcccgcagg gtccgcgagg cacgggtttt ctccgaggtc
     2041 attcctcaca ccgcgtccat cgaagagatc aaggcccgtg atccgttggc gctggtgctg
     2101 tccggcgggc ccgccagcgt gtacgccgaa ggcgccccgc agttggatcc ggcggtgttc
     2161 gacctcggtg tgccggtgtt cggcatctgc tacgggtttc aggccatggc gcaggcgctg
     2221 ggcggcaccg ttgcgcacac cggtaccagc gagtatggcc gcaccgagct gaaagtcctt
     2281 ggcggcgaat tgcattcggg cctgcccgac gtccagccgg tctggatgag tcacggcgac
     2341 gcggtcacgg ccgcgcccga gggattcgag gtggcggcca gcagcgcggg tgcggcggtg
     2401 gccgccttcg aggatcggga ccggcggctg gccggggtgc agtaccaccc ggaggtgatg
     2461 cataccccgc acgggcagca agtgctcagc cggttcctgc atgacttcgc cgggctgggc
     2521 gcccagtgga cgcccgccaa catcgccggt gccctgatcg agcaggtgcg cgctcagatc
     2581 ggcgacggct acgcgatctg cggactgtcc ggcggcgtgg attccgcggt ggccgcggcg
     2641 ctggtgcagc gggccatcgg cgaccggttg acctgtgtct tcgtcgacca cgggctgttg
     2701 cgtgccggcg agcgcgcgca ggtgcagcgg gacttcgtgg ccgccaccgg cgccaacctg
     2761 gtcaccgtcg acgccgcgga gaccttcctg gaggcgctgt cgggtgtgac caaccccgag
     2821 ggcaagcgca agatcatcgg ccgccagttc atccgggcgt tcgagggtgc ggtgcgggac
     2881 atcatgagcg acattgtgaa cgacatcgtg agcgacaaca gcattgattt cctggtgcag
     2941 ggcacgctgt atccggatgt ggtggagtcc ggcgggggca gcggcaccgc gaacatcaag
     3001 agccaccaca atgtcggcgg tctgcccgac gacctgaagt tcaagctcgt cgagccgctg
     3061 cggctgctgt tcaaagacga ggtgcgcgcg gtcgggcggg agttgggcct gccggaggaa
     3121 atcgttgcgc gccagccgtt cccggggccc ggactgggca tccggatcgt cggcgaggtc
     3181 acggctgacc ggctggacac gttgcggcgc gccgactcga tcgcgcgcga ggaactgacc
     3241 gcggccgggc tggacaacca gatctggcag tgcccggtgg tgctgctggc cgacgtccgc
     3301 tcggtcggcg tgcagggcga caaccgcacc tacgggcacc cgatcgtgct gcggccggtg
     3361 tccagcgagg acgccatgac cgccgactgg acccgggtgc cctacgaggt gctggagcgc
     3421 atctcgacgc ggatcaccaa cgaggtggcc gaggtcaacc gcgtggtgtt ggacatcacc
     3481 agcaaaccgc cgggcaccat cgagtgggag taggctcgcg ggcgctctgc gccgaacgtg
     3541 cggccagccg cacactcgag gccgaacgtg cggccagccg caccctcgag ggcgtggcgc
     3601 cggaaaagtg ccgcaaaaaa acaccatcag ccacaccagc accgagcgca taaagccgcc
     3661 tctcacgttt cttgacgctt gtcggagggt gggtgtttac tccaatctat cgaacatgcg
     3721 ttcgaatatt cgtttcggag agtgctgcag gaggcggacc atgaccacgg ctttcgcctc
     3781 cgaccaccgc gctgagcagc tcgaatcgct gcgtcggcag atggctggcg tgtccgaaaa
     3841 gatgtccgga aaggtgcccc gaagtgtcgt caccggcgcc gatgacctgt tgccggaatc
     3901 ggaagctcgg ctgccgctcc cacagtggct cgcggagtca ttgccggtcc agttaccccg
     3961 gggaacggtg gcggtgttgt cgggggcccg gtcgct
//
LOCUS       DASSGQ010000305         4728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1420391,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000305 DASSGQ010000000
VERSION     DASSGQ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420391"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1562)
                     /locus_tag="VFI55_13480"
     CDS             complement(<1..1562)
                     /locus_tag="VFI55_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7075078.1"
                     /translation="MIRRRDAASEDLPRWFPRSLRRQLLVGVLVVVSVVLMTVGIVSV
                     LNLRGYVTAMSDADVAESLDALSNSYTRYRNGDHGAGHRNTRPIAQAMLEFTDQTPGN
                     LIAVLHDGVVIGSAVFSDAEARPAPADVVHTLEAQSWPDDAPPRTEKLGALGSYRVDS
                     RIDGSDLLVVGVSLARANQIIARKNLTTAALLAAALAVTAGLTVWLVGYTMRPLRRLA
                     ATAAEVAAMPLTDGDHRISVRVRPRDTDPDNEVGIVGHTLNRLLDNVDGALAHRADSD
                     LRMRQFITDASHELRTPLAAIQGYAELTRQDSTALPPTTEYALARIESEAQRMALLVD
                     ELLLLSRMGEGEDLQTQDVDLADLVSNAVNDAAVAAPTHHWVKDLPDEPVWVRGDHTR
                     LHQLVSNLLGNARVHTPSGVTVTTKITCHRGAPHASYTELTVADDGPGIDADLLPRLF
                     ERFARADTARSDGSGNGLGLAIVSSIVKAHHGSVTAESTGRRTVFRVRLPMIDDPLAD
                     AGWPYPDPSSSKS"
     gene            complement(1559..2332)
                     /locus_tag="VFI55_13485"
     CDS             complement(1559..2332)
                     /locus_tag="VFI55_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7075079.1"
                     /translation="MTAMTGYASSQRPRQAILGQLPRIYRADGSPIRVLLVDDEPALT
                     NLVKMALHYEGWVVEIAHNGREALAKFDSVNADVLVLDIMLPDVDGLRILQRIRESDV
                     YTPTLFLTARDSVMDRVTGLTAGADDYMTKPFSLEELVARLRGLLRRSTQQTPPAAES
                     LKVGDLRIDSASREVTRDGTPVSLSSTEFELLRFLMRNPRRALSRTEILDRVWNYDFA
                     GRTSIVDLYISYLRKKIDSGREPMIHTVRGVGYMLRPPE"
     gene            2690..3187
                     /locus_tag="VFI55_13490"
     CDS             2690..3187
                     /locus_tag="VFI55_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075080.1"
                     /translation="MSGDFNVFRNKAGELALAECADGVAGLVFCGRAAISCAVAACPY
                     GPFGPDKYLWAQRFSIHRHGVSPVTPLVLAAAIALSPSPSTQPTQVVRECVWNFHVVL
                     CRWRTVDPPPDRREKVPTTSGSVQTDCAAQTVCRVISIDAGPGSMSPGDPGSAGAQIF
                     NARSI"
     gene            3227..4516
                     /locus_tag="VFI55_13495"
     CDS             3227..4516
                     /locus_tag="VFI55_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPE family protein"
                     /protein_id="HET7075081.1"
                     /translation="MDFGSLPPEVNSARMYSGPGPATLLAASAGWESLAAELNTAASC
                     YQSVISSLTDEAWTGPTSVSMAAAVTPYLSWMRAIAVQCEQAATQATAAAAAYEAAFA
                     MTVPPPLVAANRAQLMVLIATNLFGQNTPAIMATEAQYSEMWAQDATAMYNYAANSAG
                     ASAFSAFTSPPPTTNPGGVAAAQTGGNVQSKAAQLISTVPRALQSLSTPGSSSAAGTA
                     VGLGSSGATAPISALSSLTGASGKTATKTASAGAGALSGLTSSLTTALGGNVGDITDV
                     VGLGSDVAGLGADGAGLGADGGGLGFDGYGLSLDFAGLGSISGAEGVGSLPGAAGLGG
                     LGSVGGLGEGASAGLGHAASLGTLSVPSSWADALSVAPTPILNGNVMPAGAMSSLATS
                     PTVSKVPLGAMVGREADGAVQRIGFRPSVIPRSPVAG"
     gene            complement(4600..>4728)
                     /locus_tag="VFI55_13500"
     CDS             complement(4600..>4728)
                     /locus_tag="VFI55_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsxI"
                     /protein_id="HET7075082.1"
                     /translation="FITQLGRNFQVIYEQANAHGSKVQSAGSNMASTDSAVGSSWA"
BASE COUNT          783 a         1464 c         1613 g          868 t
ORIGIN      
        1 gatttcgagc tcgaggggtc cgggtatggc cagcccgcat cggcgagcgg atcgtcgatc
       61 atcggcagtc gtacccggaa aaccgttcgg cggccggttg attcggcggt caccgagccg
      121 tgatgggcct tgacgatgga actcacgatg gccaggccga gcccattgcc cgagccgtcc
      181 gatcgggcgg tgtccgcgcg ggcgaaccgc tcgaacaacc ggggcagcag gtccgcgtcg
      241 atgccgggcc cgtcgtccgc caccgtcagt tccgtgtagg acgcgtgcgg tgcgccgcgg
      301 tgacaggtga tcttcgtcgt caccgtgact ccggatggcg tatggacccg agcattgccg
      361 agcagattgc tgaccagttg gtgtagtcgg gtatggtcgc cgcgaaccca aaccggttcg
      421 tcgggcaggt ctttgaccca gtggtgggtc ggcgccgcga ccgccgcgtc gttcaccgca
      481 ttgctcacga ggtcggccag gtcgacgtct tgggtctgca gatcttcgcc ttcgcccatg
      541 cgggagagca gcagtagttc gtcgacgagc agcgccatgc gctgtgcttc ggactcgatg
      601 cgggccagcg cgtattcggt tgtcggcggt aacgccgtac tgtcctggcg ggtcaactcg
      661 gcatatccct gaatcgcggc cagcggcgtt cgcaactcat ggctggcgtc ggtgatgaat
      721 tgccgcatcc gcaagtcgga gtcggcgcga tgggccagcg cgccatccac attgtccagc
      781 aaccgattca gcgtgtgccc gacgatcccg acttcgttgt ccgggtcggt gtcccgcggc
      841 cgcacccgga cgctgatccg gtggtcgccg tcggtcagcg gcatcgcggc gacttcggcg
      901 gccgtcgcgg cgaggcggcg cagcgggcgc atggtgtatc cgaccagcca aaccgtcagc
      961 cccgcggtaa ccgccagggc cgcggcgaga agcgctgcgg tggtgaggtt tttgcgggca
     1021 atgatctgat tggcgcgcgc cagcgatacc ccgacgacca ggaggtcgga tccgtcgatg
     1081 cggctgtcaa cccgatacga acccagggcg ccaagttttt cggtccgtgg cggcgcgtcg
     1141 tcgggccacg actgcgcttc gagggtatgg accacgtcgg ccggcgcggg tcgcgcttcg
     1201 gcatcggaaa agaccgccga accgatcacc acgccgtcgt gcagcacggc aatgaggttg
     1261 cccggcgtct ggtcggtgaa ctccagcatc gcctgcgcga tcggccgggt gttgcggtgt
     1321 ccggcgccat ggtcgccgtt gcggtatctg gtgtaggagt tgctcagtgc atcaagggat
     1381 tcggcgacgt cggcgtcgct catcgcggtg acatagccgc gcaggttcag cacggagacg
     1441 atgccgaccg tcatcagcac cacgctgacg acaaccaaca cgcccaccag caactggcgg
     1501 cgcaacgagc ggggaaacca ccgtgggaga tcctcggatg cggcgtcccg ccggcggatc
     1561 attccggcgg ccgcagcatg tatccaacgc cacggacggt atggatcatc ggctcccggc
     1621 cggagtcgat cttcttgcgc aagtacgaga tgtacaggtc cacgatgctg gtgcgcccgg
     1681 cgaagtcgta gttccagacg cgatcgagga tttcggtgcg gctcaaggcg cggcggggat
     1741 tgcgcatgag gaagcgcagc aactcgaact cggtcgagga gagcgatacc ggcgtgccgt
     1801 cgcgggtgac ctcgcggctg gcactgtcga ttctgaggtc gccaaccttg agggattcgg
     1861 cggcaggcgg ggtctgctgg gtcgagcggc gcaacaaccc ccgcagccgg gcgaccagct
     1921 cctcgaggct gaacggcttg gtcatgtagt cgtcggcgcc ggcggtgagg ccggtgacgc
     1981 gatccatgac cgaatcgcgg gcggtgagga acagtgtggg ggtgtagacg tcggattcgc
     2041 ggatccgctg cagaatccgc aacccgtcca cgtcgggaag catgatgtcg aggaccagga
     2101 cgtcggcatt gacgctgtcg aacttcgcca gcgcctcgcg accattgtgg gcgatctcga
     2161 cgacccagcc ttcgtagtgc agcgccattt tcaccaagtt ggtcagcgcg ggctcgtcgt
     2221 cgaccagcaa aacccggatt ggcgacccgt cggcgcgata gatccgcggc agttgtccaa
     2281 gaatggcctg gcgtggccgc tggctgctgg catatcccgt cattgcggtc atgctcctac
     2341 attctcgcaa gcgcacacgc agttgtcacg aacttcatag agttctcaaa tataaccgag
     2401 ttggatggct cccgccggcg accgctcaaa tatgtgtttc atatgtgctg catctgccaa
     2461 cttgagatga attgcttctc caaaaatact ggaatacata gccgttggct cgatggccat
     2521 aatcaagtac cggaaactgt cctcgaaatc atcggcgcac gaacggattt cgtccgatga
     2581 cgaaacgccg agcttgggcg ttcgtcgtcg gacgcccatg acgtgcgcgc gacggctgag
     2641 gacagtgccg tcctcgactt cgttaacgtg atgttactca aagttacgaa tgtcaggaga
     2701 tttcaatgtc tttcgtaaca aagcaggcga gcttgccctc gctgaatgcg ctgatggcgt
     2761 tgcagggctt gtgttctgcg gacgagccgc catctcatgc gcggtcgccg catgtcccta
     2821 tggccccttt ggccccgata aatacctttg ggcacaacgc ttcagcattc atcgccacgg
     2881 tgtttcgccg gtgactccgc ttgtgctggc ggcggctatc gcactgtcgc cgtcgccgtc
     2941 gacacaaccg acacaagtgg tgcgcgagtg tgtgtggaac ttccatgttg tcctttgccg
     3001 ctggcgcacg gtggacccgc caccggatcg cagagaaaag gtgccgacga caagcggctc
     3061 ggttcagacc gactgcgcgg cgcagacggt ttgccgggtg atttcgatcg atgcggggcc
     3121 aggctccatg tcgcccggcg acccgggatc ggccggcgcc cagattttca atgcacgttc
     3181 gatctgacgg gccagcaaac tcaaccacac agatagggcg taggcgatgg attttgggtc
     3241 attgccgcca gaggtcaact ccgcgcggat gtattcgggt ccggggccgg cgactctgct
     3301 ggccgcctcc gcggggtggg agtcgctggc cgccgagttg aataccgccg cgagctgcta
     3361 ccagtcggtg atctcgagcc tgacggacga ggcgtggacg ggtccgacgt cggtgtcgat
     3421 ggccgcggcg gtcacgccgt acctgtcgtg gatgcgggcc atcgctgtcc aatgtgagca
     3481 ggcggccact caggccaccg ccgccgcggc cgcctatgaa gcggcgttcg cgatgacggt
     3541 gccgccgccg ctggtcgccg ccaaccgtgc tcagctcatg gtgctgatcg cgaccaatct
     3601 gttcgggcag aacaccccgg cgatcatggc caccgaggcc cagtacagcg agatgtgggc
     3661 gcaggatgcc accgcgatgt acaactacgc ggccaattcg gctggcgctt cggcattcag
     3721 cgctttcacc tcgccgccac cgaccaccaa tccgggcggg gttgccgccg ctcagaccgg
     3781 cggcaacgtc caatcgaaag cggcgcaatt gatttcgacg gtgcccagag ccctgcaaag
     3841 tctgtcaact ccgggatcgt cgtcggcggc gggcaccgcg gtaggactgg gttcctcggg
     3901 tgccaccgca ccgatcagtg cactatcgag cctgaccggc gcatcgggca agaccgctac
     3961 caagactgcc agtgcggggg ctggggccct gtccggcctg acctcaagcc tgactaccgc
     4021 gttgggaggg aacgtgggcg acataactga cgtggtcggc ctgggctccg atgtggctgg
     4081 cctcggcgcc gatggcgcag gcttgggtgc cgacggtggc ggacttggct tcgacgggta
     4141 cggactttcg ttggatttcg cgggtttggg ttcgatttcc ggagccgagg gagtcgggag
     4201 tctcccgggc gccgcgggcc tcgggggcct gggctcggtg ggaggtttgg gcgaaggagc
     4261 gtccgcgggc ctcggtcatg cggcctcgct gggcacgttg tcggtgccat cgagttgggc
     4321 cgacgcgctg tcggtggcac cgacgcccat actcaacggc aacgtcatgc ccgccggtgc
     4381 gatgtcgtcg ctggcgacca gtcccaccgt ttccaaggtg ccgttgggcg ccatggtcgg
     4441 acgcgaggcc gacggtgcgg ttcagcgaat cgggttccgc ccctcggtga ttccgcgttc
     4501 acccgtggcc gggtgaacgc gcacaccgtg agagcgcaga cgtcaaaaaa ccggggccgc
     4561 acaccgatcg gtgtgcggcc ccggtctcga gaagcggtgt caggcccagc tggagccgac
     4621 ggcgctgtcg gtgctggcca tgttgctgcc ggcgctttgc accttcgagc cgtgggcgtt
     4681 ggcctgctcg tagatcacct ggaagttacg acccaactgg gtgatgaa
//
LOCUS       DASSGQ010000306         9382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_113325,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000306 DASSGQ010000000
VERSION     DASSGQ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9382
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113325"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VFI55_13505"
     CDS             complement(<1..349)
                     /locus_tag="VFI55_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7075083.1"
                     /translation="MRVDFTPDPQLYPFESRWFDSSRGRIHYIDEGEGPPLLLCHGNP
                     TWSFLYRDIVVALRDRFRCIAPDYLGFGLSDRPPGFGYKIEEHATVIGEFVDHLGLDG
                     YLTFGQDWGGPISM"
     gene            complement(436..2598)
                     /locus_tag="VFI55_13510"
     CDS             complement(436..2598)
                     /locus_tag="VFI55_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7075084.1"
                     /translation="MGRHSMPGPEDSLDEPSDEQLADYEDAGEDTGQHHIGASHEDVA
                     EHPGPAPDYRDGWDAGGLLSGEEGFRADEHEQADADGPGGGDHHPGQADLFAGHDPYP
                     VGGGFDAGAEYPEFPQRQPGAGSAEPPVAPPPFDGGHRGLGGWPGGHRSQGGRRGVSI
                     GLIVVLVAVVVVVGTVILWRFFGNALSNRSHEAAGRCVGGNETVAVIADPSIADPVRQ
                     LAESFTSSTGPIGDHCMVVSVKPAGSDAVVGGFAGKWPADLGGQPALWIPGSSISAAR
                     LAAGAGQKAITDSRSLVTSPVLLAVRPELAGALANQNWAALPGLQTNPNSLAGLNLPA
                     WGSLRLALPTNGNGDASYLAGEAVAAASAPAGAPPTQGTGAVRALLSTQPKLADNSLA
                     EAMNTLLKPGDAATSPVHAVITTEQQLFQRGQSLSDAKSTLGSWQPPGPVPVADYPTV
                     LLSGSWLTQEQSSAASEFARYMHKPEQLAKLAKAGFRVNGVQPPSSAVTSFSALPSTL
                     SVGDDATRATLAEAMAAPSGGPAATIMLDQSMPNDDGGKTRLANVIAALEDKFKALPP
                     TAVVGLWTFDGHEGRPEVTTGPLGDPVNGQPRSAAVTAALDRQYSSAGGAVAFTTLRL
                     IYQDMQANYRAGQTNSILVITAGPHTDQTLDGPGLQDFIRKAADPSKPIAVNVIDFGA
                     DPDRATWEAVAQLSGGGYQNLGTSASPDFAAAVNTFLS"
     gene            complement(2711..4912)
                     /locus_tag="VFI55_13515"
     CDS             complement(2711..4912)
                     /locus_tag="VFI55_13515"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HET7075085.1"
                     /translation="MTDRVSVGNLRVARVLYDFVNNEALPGTDIDPDSFWAGVDKVVA
                     DLTPRNQELLSRRDELQAQIDRWHRQRVIGPMDPDAYRQFLAEIGYLQPEPDDFTITT
                     AGVDPEIATTAGPQLVVPVTIARFALNAANARWGSLYDALYGTDVIPEADGAEQGTSY
                     NKVRGDKVIAYAREFLDQAVPLEKGSWTDATGLSIVDGQLQIATGAGATALASPDKFV
                     GYSGELGSPDWSVLLVDHGLHIEILIDPESPIGKTDAAGIKDVVLESAVTTIVDFEDS
                     VSAVDADDKVLGYRNWLGLNKGDLAEEVNKGGKTFTRVLNQDRTYTTPDGGGSITLPG
                     RSLLFVRNVGHLMTNDAIVSGDGAGDEKEIFEGIMDALLTGLIAIHGLKTAAANGPLA
                     NSRTGSIYIVKPKMHGPDEVAFTCELFSRVEDVLGLPQATLKVGIMDEERRTTLNLKA
                     CIKAAADRVVFINTGFLDRTGDEIHTSMEAGPMIRKGAMKSTTWIKAYEDANVDTGLA
                     AGFKGKAQIGKGMWAMTELMADMVEQKIGQPKAGATTAWVPSPTAATLHAMHYHYVDV
                     GAVQAELEGKKRTTIDELLTIPLAKELAWASEEIREEVDNNCQSILGYVVRWIDQGVG
                     CSKVPDIHNVALMEDRATLRISSQLLANWLHHGVITSKDVRASLERMAPMVDQQNAGD
                     AAYRPLAPNFDDSIAFLAAQELILSGTQQPNGYTEPILHRRRREAKSRSGA"
     gene            complement(5128..6168)
                     /locus_tag="VFI55_13520"
     CDS             complement(5128..6168)
                     /locus_tag="VFI55_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET7075086.1"
                     /translation="MKDALGVLLSIVLIGVNAFFVGAEFSLISARRDRLEALAEQGNA
                     RAVTVIRAGQQLSSMLAGAQLGVTVASLLLGRIGESAVASLLRDTLGLTGMHPALLHT
                     LSLAIALAIVVTLHVLLGEMVPKNIALAGPERTAMLLVPPYLAYVRAARPFIVFYNRC
                     ANVVLRALRVEPKDELDVAVSSAELGDLIAESVSEGLLDSEEHTRLTRALQIRSRVVG
                     DVAVPITDVRAVPVAAAGSGPVVGALEEALAQSGYSRFPVVDLDGQFIGYLHIKDVLK
                     LDDDPKTVIDLALVRPLPLVRASLSLGDALSRLRRSNSHLALVDDDSGAVVSMVAMED
                     LVEDLVGTARDA"
     gene            complement(6165..7532)
                     /locus_tag="VFI55_13525"
     CDS             complement(6165..7532)
                     /locus_tag="VFI55_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET7075087.1"
                     /translation="MNIAVTVISVLIIALLIFGNAVFVAAEFSLTALDSSVVEANARR
                     GGRRDRLIQRAQHRLSFQLSGAQLGISITTLATGYLTEPMVADLPHPRMTAIGIPDGL
                     AEAIIAFLALVIVTSVSMVFGELVPKYVAMARPLATARAVVGVQMLFSLLCTPAIRLT
                     NGAANWIVRKLGVEPAEELRSARSPQELVSLVRNSARSGTMDAATAVLVRRSLQFGGL
                     TAEELMTPRSKIVALQTDDTVADLVAAAAESGFSRFPIVDGDLDETVGIVHVKQVFAV
                     PPADRAHTLLTALAQHVPVVPSTLDGDAVMAEIRANNLQTAMVVDEYGGTAGMVTVED
                     LIEEIVGDVRDEHDDATPDVVVAGNGWQVSGLLRIDEVDTAIGYRAPEGPYETIGGLV
                     LRELGHIPVAGETVELPALDADGLPDTSIRWRATVVRMDGRRIDLVELTELDSRTAPA
                     GRGPR"
     gene            complement(7779..9215)
                     /locus_tag="VFI55_13530"
     CDS             complement(7779..9215)
                     /locus_tag="VFI55_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB1 family IMP dehydrogenase-related protein"
                     /protein_id="HET7075088.1"
                     /translation="MRFLNGHRPGYDLTYNDVFIVPNRSDVASRFDVDLSTGDGSGTT
                     IPVVVANMTAVAGRRMAETIARRGGIVILPQDLPITAVQQTVEFVKSRDLVLDTPVVL
                     APDDSVSDATALIHKRAHGVAVVAFEGRPIGLVSESSCLGVDRFTRVRDVAVTDFVTA
                     PAGAEPRKIFDLLEHAPIGVAVVTNPDGTLAGVLTRTGAIRAGLYTPATDARGRLRVG
                     AAIGINGDVGAKARSLAEVGVDVLVIDTAHGHQVKTLDAIKVVSELDLGLPLAAGNVV
                     SAAGTRDLLAAGANIVKVGVGPGAMCTTRMMTGVGRPQFSAVVECASTAKELGGHVWA
                     DGGVRHPRDVALALAAGASNVMIGSWFAGTYESPGDLMRDRDNQPYKESYGMASKRAV
                     VARSGGDTAFDRARKALFEEGISTSRMELDPDRGGVEDLIDHITSGVRSTCTYVGAST
                     LAELHERAVIGVQSTAGFAEGHPLPLGW"
     gene            complement(9261..>9382)
                     /locus_tag="VFI55_13535"
     CDS             complement(9261..>9382)
                     /locus_tag="VFI55_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075089.1"
                     /translation="ALTQGLRDFFGAHTYGRIDADPDKRFHTLWSGDRSEVPA"
BASE COUNT         1517 a         3225 c         3078 g         1562 t
ORIGIN      
        1 ccatgctgat cgggccgccc cagtcctggc caaaggtcag gtagccgtcg aggccgaggt
       61 ggtcgacgaa ctcgccgatc accgtcgcgt gctcctcgat cttgtacccg aaccccgggg
      121 gacggtccga aagcccgaaa ccgagataat ccggtgcgat gcaacggaac cggtcgcgca
      181 atgcgacgac gatgtcgcga tacaggaaac tccaggtggg attgccgtgg cacaacagca
      241 gcggcggacc ctcaccctca tcgatgtagt ggatacgacc ccgcgagctg tcgaaccatc
      301 gcgactcgaa tggatataac tggggatccg gggtgaaatc gacacgcata acgctccttt
      361 gacagcgccc acgatcgtat gcccgaagtg tgacatcccc accgtccgag accggtgcga
      421 cggtgcgacc ggctatcaac tcaaaaacgt gttgaccgcc gcggcgaagt ccggtgacgc
      481 cgatgtgccg aggttctggt agccgccgcc gctgagctgg gcgacggctt cccaggtcgc
      541 ccggtcggga tcagcgccga agtcgatgac gttgaccgcg atcggtttgc tcgggtcggc
      601 ggccttgcga atgaagtcct gcaaccccgg cccgtccaaa gtttggtcgg tgtgcggccc
      661 ggcggtgatg accaatatcg aattcgtttg accggcacgg taattcgcct gcatatcttg
      721 gtagatgagc cgcagcgtgg tgaacgccac cgctccgccg gccgacgaat actgcctgtc
      781 cagcgcggcc gtcaccgccg ccgagcgcgg ctgtccgttg accgggtcgc ccagcggccc
      841 ggtcgtcacc tcggggcgac cctcgtggcc gtcgaacgtc cacagcccca caacggcggt
      901 gggcggcagc gctttgaact tgtcctcaag cgcggcgatc acgttggcca gccgcgtctt
      961 tccgccgtcg tcgttgggca tcgactggtc gagcatgatg gtcgcggccg gcccgcccga
     1021 cggcgcggcc atggcttcgg ccagcgtggc gcgcgttgcg tcgtcgccca ccgacaatgt
     1081 ggagggcagg gccgaaaagc tggtgaccgc gctgctcggc ggttggaccc cattgacccg
     1141 gaagcccgcc ttggccagct tcgccagttg ctcgggtttg tgcatgtagc gggcgaactc
     1201 gctggccgcc gagctctgct cctgcgtcaa ccacgacccg ctgagcagca ccgtgggata
     1261 gtcggccacc ggcaccggtc ccggcggttg ccaggaaccc agcgtgctct tggcgtctga
     1321 cagggactgg ccgcgctgga acagctgctg ctcggtggtg atcaccgcat gcactggcga
     1381 ggtcgccgcg tcgccgggct tgagtagcgt gttcatcgcc tctgccagcg agttgtccgc
     1441 caatttgggc tgtgtgctca gcaacgcgcg cacggcaccg gtgccctggg tcggcggcgc
     1501 acccgcgggg gccgacgcgg ccgccaccgc ctcgccggcc aagtacgatg cgtcaccgtt
     1561 gccgttcgtc ggcagcgcca gtcgcagcga tccccacgcc ggtaagttca acccggccaa
     1621 cgagttcggg tttgtttgca gaccgggcag cgccgcccag ttctggttgg ccaaggcgcc
     1681 cgcgagttcc ggccggacgg cgagcagcac cggcgacgtc accagcgaac ggctgtcggt
     1741 gatcgctttc tgacccgcgc ctgcggcgag ccgcgcggcc gagatcgagc tgcccggaat
     1801 ccacaatgcc ggctggccac ccagatcggc gggccacttg ccggcgaaac cgccgacgac
     1861 ggcgtcggag ccagccggtt tgacgctgac caccatgcag tggtcaccga tcgggccggt
     1921 cgacgaggtg aagctttccg ctagctgccg caccggatcc gcgatcgacg ggtcggcgat
     1981 cacggcgact gtttcgttgc ctcccacgca gcgtccggcg gcctcgtgcg accggttgga
     2041 caacgcgtta ccgaagaacc tccacaggat cacggtgccg accaccacga caacggcgac
     2101 caggacgacg atcaggccga tgctgacgcc ccgccgcccg ccttgactgc ggtggccgcc
     2161 cggccaaccc ccgagtcccc gatggccgcc gtcgaacggc ggcggagcga ccggtggctc
     2221 ggcgctcccc gcgcccggtt gccgctgcgg aaactccgga tactcggcgc cggcgtcgaa
     2281 cccaccgccg acggggtagg ggtcgtggcc cgcgaagaga tcggcctggc cggggtggtg
     2341 atcgcccccg ccggggccgt ccgcatccgc ctgctcgtgc tcgtcagcgc ggaagccttc
     2401 ctcacccgag aggaggcccc cggcgtccca gccgtcgcgg taatcgggcg ccggccccgg
     2461 gtgttcagca acatcctcgt gactagcacc gatgtggtgt tggcccgtgt cttcgccggc
     2521 gtcttcgtag tcggcaagct gttcgtcgga gggctcgtcg agggaatcct ccgggccggg
     2581 catgctgtgc ctacccatag cggtgcccgc cgcctttccg ttgattcgtc gcctgagccc
     2641 ttgtgaactc ggcgccgagt ctaaccatta gacgggtgac tgacgggtat tgccgcacgt
     2701 gccgcccgaa ttaggcaccg gagcgtgatt tggcttcacg tcgacggcga tgcaatatcg
     2761 gctcggtgta gccgttgggc tgctgggttc cggacaagat cagctcctgg gcggccagga
     2821 aggcgatgct gtcgtcgaaa ttgggcgcca agggccgata tgccgcgtcg ccggcgtttt
     2881 gttgatccac catcggagcc atccgttcca ggctggcccg gacgtccttg ctggtgatca
     2941 cgccgtggtg cagccagttg gccagcagct ggctggaaat ccgcagggtg gcgcggtcct
     3001 ccatgagcgc cacgttgtga atgtcgggca ccttcgagca gccgacgccc tgatcgatcc
     3061 agcgcaccac gtagccgagg atggactggc agttgttgtc gacctcctcg cggatctcct
     3121 cggaagccca ggccagttcc ttggccagcg gaatcgtcag cagctcgtcg atggtggtgc
     3181 gtttcttgcc ttcgagctcg gcctgcaccg cgcccacatc cacatagtgg taatgcatcg
     3241 cgtgcagggt ggccgccgtc ggcgagggca cccacgcggt ggtggcgccg gccttgggct
     3301 ggccgatctt ctgctcgacc atgtcggcca tcagctccgt catcgcccac atgcccttgc
     3361 cgatctgggc cttgcccttg aacccggcgg cgagcccggt gtcgacgttg gcgtcctcgt
     3421 aggccttgat ccacgtggtg ctcttcatcg cgcccttgcg gatcatcggg ccggcttcca
     3481 tcgaggtgtg gatctcgtcg ccggtgcggt cgaggaatcc ggtgttgatg aacacgaccc
     3541 ggtcggcggc ggctttgatg cacgccttca agttgagcgt cgtacgtcgc tcttcgtcca
     3601 tgatgccgac cttcagcgtg gcctgcggta ggccgaggac gtcttcgaca cggctgaaca
     3661 gctcgcaggt gaacgcgacc tcgtcggggc cgtgcatctt gggtttgacg atgtagatgg
     3721 agccggtgcg gctgttcgcc agtggcccgt tggctgctgc ggtcttgagt ccatggatcg
     3781 cgatcaggcc ggtcagcagc gcgtccatga tgccttcgaa gatctctttt tcgtcgccgg
     3841 caccgtcgcc gctgacgatc gcgtcgttcg tcatcaggtg gccgacgttg cggacgaaca
     3901 gcaggctgcg tccgggcagg gtgatcgacc cgccgccatc cggcgtggtg taggtgcggt
     3961 cctggttgag cacgcgggtg aacgtcttgc cgcccttgtt gacctcctcg gccaggtcac
     4021 ccttgttcag cccgagccaa ttgcggtagc cgagcacctt gtcgtcggcg tccacggcgg
     4081 acacggagtc ctcgaagtcg acgatcgtgg tgacggcgga ctcgaggacc acgtccttga
     4141 tgcctgcggc gtcggtcttg ccgatggggg actcggggtc gatcaggatc tcgatgtgca
     4201 ggccgtggtc gaccagcagc accgaccagt cgggggagcc gagctcaccg ctgtacccga
     4261 cgaacttgtc cggactggcc agcgcagtgg cccccgcgcc ggtggcgatc tgcagctgcc
     4321 cgtcgacgat gctcaggccg gtcgcgtccg tccacgagcc cttctccagc gggacagcct
     4381 gatcgagaaa ctcgcgggcg taggcgatca ccttgtcgcc gcggaccttg ttgtaactgg
     4441 tgccctgttc ggcgccgtcg gcttccggaa tgacatcggt gccgtagagg gcgtcgtaga
     4501 gcgagcccca ccgggcgttg gccgcgttga gggcgaaccg ggcaatggtc accggcacca
     4561 ccagctgagg accggccgtc gtggcgatct cgggatcgac gccggccgtg gtgatggtga
     4621 agtcgtcggg ctcaggctgc agatagccga tctcggcgag gaactgacgg tacgcgtcgg
     4681 ggtccatcgg cccgatcacc cgctgccggt gccacctgtc gatctgggcc tgcagctcgt
     4741 cgcggcggct cagcaattcc tggttccgcg gcgtgaggtc ggcgaccacc ttgtcgacgc
     4801 cggcccagaa gctgtccggg tcgatatcgg tgccagggag ggcctcgttg ttcacgaagt
     4861 cgtagagcac ccgggcgacg cgcaagttcc ccaccgacac gcgatcagtc atctttctcc
     4921 tccatcatgg gccaagttcc accgcaccgg ccctcaataa gcgctgtcgt cagcctacct
     4981 accggcaacc tgacaaacgt catccggccg caccgcaaca gcaggtcacg ccccgctggc
     5041 agctttgacg gtggtgcccc agcgagcccg gggttgatgg ccgttggaga tcatacgggt
     5101 acccgacgct acggggcggg ggtcgcacta tgcgtcgcgg gcggtgccga ccaggtcttc
     5161 caccaagtcc tccatcgcga ccatcgagac gacggcgcca ctgtcgtcgt ccaccagtgc
     5221 caggtggctg ttgctgcggc gcagccgaga cagggcatcc cccagcgaca acgacgcgcg
     5281 aacaaggggc agcggtcgga ccaacgccag atcgatcacc gttttcgggt catcgtcgag
     5341 cttcagcacg tccttgatgt gcagatagcc gatgaactgc ccgtcgagat cgaccaccgg
     5401 aaaacgggag tagccgctct gggccagtgc ttcttccaaa gcgccgacca ccggcccgga
     5461 gccggccgcg gcgaccggga ccgctcggac atcggtgatt ggcaccgcca cgtcgccgac
     5521 cacccggctg cgaatctgca gcgcccgggt caaccgggtg tgttcctcgc tgtccagcag
     5581 gccctcggac accgattcgg cgatcaaatc gcccagctcg gcgctggaga ccgcgacgtc
     5641 gagttcatcc ttcggctcga cgcgcagcgc gcgcagcacg acgttggcgc acctgttgta
     5701 gaacacgatg aacggcctgg ctgctcgcac gtaagccagg tagggcggta ccaacagcat
     5761 cgcggtgcgc tccggaccgg ccagcgcgat gttcttcggc accatctcgc ccagcagcac
     5821 gtgcagcgtc accacgatcg ccagcgcgat cgccaaggac agcgtgtgca ggagcgccgg
     5881 gtgcattccg gtcagcccca gcgtgtcccg cagcaggctg gcgaccgcgg actcgccgat
     5941 ccggccgagc agcagtgagg cgaccgtgac gcccaattgg gcgccggcga gcatggacga
     6001 gagctgctga ccggcgcgga tgacggtgac ggctcgcgcg ttgccctgct cggccagcgc
     6061 ttccaggcgg tcccgccggg ccgagatcag cgagaattcc gcccccacga agaacgcgtt
     6121 caccccgatc aacacgatcg agagcagcac tcccagggcg tccttcatcg ggggccccgt
     6181 cccgcgggcg cagtacgact gtccagctcg gtcaactcga ccaggtcgat ccggcgaccg
     6241 tccatccgaa ccaccgttgc ccgccagcgt atcgaggtat ccggcagccc gtccgcgtcc
     6301 aacgcgggca gctctaccgt ctccccggcc accgggatgt ggccgagctc gcggagtacc
     6361 aggccgccga tcgtctcgta tggcccttcc ggggctcgat atccgatggc ggtgtccacc
     6421 tcatcgattc gcagcaggcc cgatacctgc cagccgttgc cggcaacgac cacatcgggg
     6481 gtggcgtcgt cgtgttcgtc gcggacgtca cccacgatct cttcgatcaa atcctcgacc
     6541 gtcaccatgc ccgccgtgcc gccgtattcg tcgacgacca tcgccgtttg cagattgttg
     6601 gcacggatct cggccatcac cgcatcgccg tccagcgttg acgggaccac cggaacgtgt
     6661 tgcgcgagcg cggtcagcag ggtgtgcgca cgatcggccg gtggaaccgc aaacacctgt
     6721 ttgacgtgca cgatgccgac ggtctcgtcg agatcgccgt cgacgatggg gaaacgcgag
     6781 aagccggact cggcggccgc ggcgaccaag tcggcgacgg tgtcgtcggt ctgcagcgcg
     6841 acaatcttcg acctcggcgt catcagttcc tcagcggtca ggccgccgaa ctgcagcgat
     6901 cgccgcacca ggactgcggt ggcggcgtcc atggtgccgc tgcgcgccga gttacgcacc
     6961 agtgacacca gttcctgcgg tgaccgggcc gaccgcagct cctcggccgg ctcgacgccc
     7021 agcttgcgca cgatccagtt tgccgcgccg ttcgtcaatc ggatagccgg cgtgcacagc
     7081 agcgagaaca gcatctgaac gcccacgacg gcacgcgcgg tcgcaagcgg gcgcgccatg
     7141 gcgacatatt tcgggaccag ctcaccgaac accatcgaca ccgacgtcac gatcaccagc
     7201 gccaggaacg cgatgatcgc ctcggccagc ccgtccggta tcccgatcgc ggtcatcctc
     7261 gggtggggca ggtcggccac catcggctcg gtcaggtatc cagtcgccag cgtggtgatc
     7321 gagatgccca gttgagcacc cgacagctgg aacgacagcc ggtgttgggc gcgctgaatg
     7381 aggcggtcac ggcgcccacc gcggcgggcg ttggcctcca cgacgctgct gtccagggcg
     7441 gtcaacgaga attcggccgc gacgaagact gcgttgccga agatgagcag cgcgatgatc
     7501 agcacgctga tcacggtgac tgcgatgttc atgacgcccc ggggaacgct gggaacgcag
     7561 tgggaggccg cgtgctacgc aaaacggcct tgacgcgtgc ctgcggcacg tcatcccttt
     7621 ccctcgatgc cgcgcggcgc tttgccacgg gcccaaaact cacgtgaatt cacatggtag
     7681 ccaagacgtc caatcgacca ggcacgaaca ttgccgacga ggcgatcttc ttacgatcct
     7741 cagatcgtgg cgtcaacccg tcaccaaccc aaaggacgtc accaacccaa cggcagcgga
     7801 tggccttcag cgaagcccgc ggtcgactgc acaccgatga cggcccgctc atgcagctca
     7861 gccagggtcg aggcacccac gtaggtgcac gtgctgcgca cgccggaggt gatgtggtcg
     7921 atcagatcct cgacgccgcc gcggtcgggg tccagctcca tccgagacgt cgagatgcct
     7981 tcctcgaaca aggccttgcg ggcgcgatcg aacgcggtgt ccccgccgct acgggccacc
     8041 accgcccgtt tggacgccat cccgtagctc tccttgtacg gctggttgtc acggtcgcgc
     8101 atgaggtcgc ccggcgactc gtacgtgccg gcgaaccacg acccgatcat cacattcgac
     8161 gccccggccg ccagcgccaa cgccacgtcc cgcgggtgcc ggacgccgcc gtccgcccac
     8221 acgtggccac ccaattcctt tgccgtagag gcacattcga ccaccgccga gaactgcggg
     8281 cggccgactc cggtcatcat ccgggtggtg cacatcgcgc ccggcccgac accgaccttg
     8341 acgatgttcg ccccggcggc gagcaggtcc cgagtgccgg ccgccgagac cacgttgccc
     8401 gccgccagcg gcaaccccag gtccaactcg gagaccacct tgatcgcatc cagcgtcttg
     8461 acctgatgcc cgtgtgcggt atcgatcacc agcacatcga caccgacctc ggccagcgat
     8521 cgggccttgg cccccacatc cccgttgatg ccgatggcgg caccaacgcg cagccgcccg
     8581 cgcgcgtcgg tggccggggt gtacaggccc gcacggatcg ccccggtgcg agtgagcact
     8641 ccagccagcg tgccgtccgg gttggtcacc accgcgacgc cgatcggggc gtgctcgagc
     8701 aggtcgaaga ttttgcgcgg ctcggcgccc gccggggcgg tgacgaagtc cgttacggcg
     8761 acgtcgcgca cccgggtgaa gcggtccacc cccaggcagg acgattcgct gaccaaaccg
     8821 atggggcggc cctcgaaagc caccacggcg acgccgtgcg cacgcttgtg gatcagcgct
     8881 gtcgcgtcgg acaccgaatc gtcgggcgcc agcaccacgg gggtgtcgag aaccaggtcg
     8941 cgactcttga cgaactccac ggtctgctgg acggcggtga tcggcagatc ctgcggcagg
     9001 atcacgatcc cgccgcgccg cgcaatagtc tcggccatcc gccgtcccgc caccgcggtc
     9061 atgttggcaa ccacgaccgg aatggtggtg cccgagccgt cgccggtgga caaatccacg
     9121 tcgaagcgcg acgcgacgtc ggaccggttc ggcacgatga agacgtcgtt gtaggtcagg
     9181 tcgtacccgg gtcgatgccc gttcagaaac ctcatctgag ccgatttgcc ctcatcgcat
     9241 cccttatacg aaagccgcgg tcaggcgggc acttcgctgc ggtctccgct ccacagggtg
     9301 tggaatcgct tgtccgggtc ggcgtcgatg cggccgtacg tgtgcgcgcc gaagaagtcg
     9361 cgcagcccct gggtgagcgc cg
//
LOCUS       DASSGQ010000307         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1480510,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000307 DASSGQ010000000
VERSION     DASSGQ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480510"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VFI55_13540"
     CDS             complement(<1..842)
                     /locus_tag="VFI55_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HET7075090.1"
                     /translation="MADTESDTNSENHTPAAGGDVPAKKPDLTVPEMRQWLRTWVGKA
                     VGKAPDEIDESVPMVELGLSSRDAVAMAADVEDMTGVTLSVAVAFQHPTIESLATRII
                     EGEPETNDAGLDDVDWTRKGPAERVDIAIVGLSTRLPGDMNSPDATWQALLEGRDAIT
                     DLPEGRWSEFLEEPRLAKRIAQARTRGGYLTDLKGFDSEFFAVAKTEADNIDPQQRMA
                     LELTWEALEHARIPASSLRGEAVGVFVGFTNNDYGFLAIADPTVAHPYAITGNSTAII
                     ANRVS"
     gene            complement(854..>2109)
                     /locus_tag="VFI55_13545"
     CDS             complement(854..>2109)
                     /locus_tag="VFI55_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7075091.1"
                     /translation="GKEGDRGLSWLPFFHDMGLITALLSPVLGHNFTFMTPAAFVRRP
                     GRWIREMARKPDDAPDCEVFTVAPNFAFEHAAVRGVPKDDEPPLDLSNVKGILNGSEP
                     VSPSSVRKFLDAFAPYGLRDTAVKPSYGLAEATLFVSTTPMDEAPRIIYVDRGELNNQ
                     RFVEVAPDSPNAVAQVSAGVIGVDEWAIIVDPDTATELPDGQIGEIWLHGNNMGVGYW
                     GKEEETQYTFRNILKSRIPQSHAEGAADDGLWVRTGDYGTYFDGHLYIAGRIKDLVII
                     DGRNHYPQDLEYSAQEASRALRTGYVAAFSVPANQLPQLVFDNPHAGLKFDAEDSSEQ
                     LVIVAERAAGSHKLDYEPIADEIRGAIAVRHGVTVRDLLLVQAGTIPRTSSGKIGRRA
                     CRTAYLDGSLRSGIGSPTAFANATD"
BASE COUNT          312 a          691 c          735 g          371 t
ORIGIN      
        1 gacacccggt tggcgatgat cgcagtcgag ttgccggtga tggcgtaggg atgcgcgaca
       61 gtcgggtcgg caatcgccag gaagccgtag tcgttgttgg tgaagccgac gaatacgccg
      121 accgcctcgc cgcgcaggct cgacgccggg atgcgggcgt gctcgagcgc ctcccaggtc
      181 agctccaacg ccatccgctg ctgcgggtcg atgttgtcgg cttcggtctt ggccaccgcg
      241 aagaactcgg agtcgaaccc cttgaggtcc gtcaggtagc cgccgcgggt gcgggcctgc
      301 gcgatgcgct tggccagccg cggctcttcg aggaactccg accaacggcc ctcgggcagg
      361 tcggtgatgg cgtcgcggcc ttccagcagc gcctgccagg ttgcgtcggg gctgttcatg
      421 tcgccgggca gccgcgtgga caggccgacg atcgcgatgt cgacgcgctc ggccgggccc
      481 ttgcgcgtcc agtcgacgtc gtcaaggccg gcgtcgttcg tttcgggctc gccctcgatg
      541 atcctggtcg ccagcgattc gatggtcgga tgctggaacg ccaccgcgac cgacagcgtg
      601 acaccggtca tgtcctcgac gtcggccgcc atcgcgacgg cgtcccgaga cgacaagccg
      661 agctcgacca tcgggaccga ctcgtcgatc tcgtcgggtg cctttccgac ggccttgccc
      721 acccaggtgc gcagccactg ccgcatctcg ggaaccgtca ggtcgggctt cttggcgggt
      781 acgtcgccgc ccgcggccgg agtgtggttt tcggagttcg tgtcagattc tgtgtcagcc
      841 atggttcctg gattcagtcg gtcgcattgg cgaaggcggt cggggagccg atgccgctgc
      901 gcagactgcc gtcgaggtag gcagtgcggc acgcgcgtcg cccgatcttg ccgctcgagg
      961 tacgcggaat agttccggcc tgcaccagca gcaagtcgcg cacggtcacg ccgtgccgca
     1021 cggcgatggc cccccggatc tcgtcggcga tgggctcgta atcgagcttg tgcgagccgg
     1081 cggcgcgctc cgcgacgatc accagctgct cggaggagtc ctcggcgtcg aacttcaggc
     1141 cggcgtgcgg gttgtcgaac accagctgcg gcagctggtt ggccggcacc gagaaagcgg
     1201 cgacatagcc ggtacgcagc gcccggctgg cttcctgcgc cgagtactcg aggtcctgcg
     1261 ggtagtggtt gcggccgtcg atgatgacca ggtccttgat gcgccccgct atatagaggt
     1321 ggccgtcaaa gtaggtgccg tagtcgccgg tgcgcaccca cagcccgtcg tcggcggcgc
     1381 cttccgcgtg cgactggggt atgcgcgact tgaggatgtt ccggaacgtg tactgggtct
     1441 cttcttcctt gccccagtag cccacgccca tgttgttgcc gtgcaaccag atctcgccga
     1501 tctggccgtc cggcagctcg gtggccgtgt cggggtcgac gatgatggcc cactcgtcga
     1561 caccgatgac gccggcggaa acctgcgcca ccgcgttcgg cgagtccggg gcgacttcga
     1621 cgaagcgctg gttgttcaac tcgccgcgat cgacgtaaat gatcctgggc gcctcatcca
     1681 tcggggtggt ggagacgaat agcgtggcct cggccagacc gtaggacggc ttgaccgccg
     1741 tgtcccgcag gccgtagggc gcaaacgcgt ccaagaactt gcgcaccgac gacggcgaca
     1801 ccggctcgct gccgttgagg atccctttga cgttgctcag gtccagcggc ggctcgtcgt
     1861 ccttgggcac accgcgaacc gcggcatgct cgaaggcgaa gttcggcgca acggtgaaga
     1921 cctcgcagtc cggggcgtcg tcgggcttgc gcgccatctc ccggatccac cggccgggcc
     1981 ggcggacgaa cgcggcgggc gtcatgaagg tgaagttgtg accgagcacc ggtgacagca
     2041 gcgccgtgat cagccccatg tcgtggaaga acggcagcca ggacaacccg cggtcgcctt
     2101 ccttgcctt
//
LOCUS       DASSGQ010000308         7497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_903471,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000308 DASSGQ010000000
VERSION     DASSGQ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7497
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903471"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="VFI55_13550"
     CDS             <1..150
                     /locus_tag="VFI55_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HET7075092.1"
                     /translation="GAKKDTAFYQGKVATAAFFARNMLPKLTAQRRVLEALDDDVMRI
                     SESAF"
     gene            complement(258..473)
                     /locus_tag="VFI55_13555"
     CDS             complement(258..473)
                     /locus_tag="VFI55_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075093.1"
                     /translation="MPDTDAISPPTCAWPFAGGDVVAEAPGGLVVELGAVGLDVGLDV
                     VDEPHAAMDSVAAPVAASTAKRVSRGM"
     gene            463..1056
                     /locus_tag="VFI55_13560"
     CDS             463..1056
                     /locus_tag="VFI55_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HET7075094.1"
                     /translation="MSGNAIQVTQRDNGSATVDFTPSTKVTEVTPAGLTDVTTGSCVT
                     VRPSRDESQGGQSITAGSVRVSPAVDGKCPQGTQARPGSGPGSTPPAPSGSPTKAPGK
                     RPAVQGAVASVAGNTINVTTTDASGNVSQTAVTVNDKTRYTKQASATSEAISTGKCLA
                     ARGTKDGSGTLQATDVDLRPANDGNCGGMGRQPHGQR"
     gene            complement(1084..1431)
                     /locus_tag="VFI55_13565"
     CDS             complement(1084..1431)
                     /locus_tag="VFI55_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET7075095.1"
                     /translation="MSETSAPDEELRFDLEEAMRDVVDPELGINVVDLGLVYGLNVEK
                     GDEGTVALIDMTLTSAACPLTDVIEDQSRSALVGSGLVNDLRINWVWNPPWGPDKITE
                     DGREQLRALGFTV"
     gene            complement(1424..1925)
                     /locus_tag="VFI55_13570"
                     /pseudo
     CDS             complement(1424..1925)
                     /locus_tag="VFI55_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
     gene            complement(1922..3187)
                     /locus_tag="VFI55_13575"
     CDS             complement(1922..3187)
                     /locus_tag="VFI55_13575"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET7075096.1"
                     /translation="MNASAPHLDLAAIRADFPILKRIMRGGNQLAYLDSGATSQRPLQ
                     VLDAEREFLLTSNGAVHRGAHQLMEEATDGYEQGRADVAAFVGADPDELVFTKNATES
                     LNLVSYVLGDNRFGRAVGPGDVIVTTELEHHANLVPWQELARRTGATLRWYGVTDGDK
                     EAGRIDLDSLQLDERVKVVAFSHHSNVTGAVAPVAELVARANAVGALTVLDACQSVPH
                     QPVDFHALDVDFAAFSGHKMLGPNGIGVLYGRHELLSEMPPFLTGGSMIETVTMEATT
                     YAPAPQRFEAGTPMTSQVVGLAAAARYLGAIGMDAVEAHERELVAAAIAGLSGIDGVR
                     IVGPTSMENRGSPVAFVVDGVHAHDVGQVLDDDGIAVRVGHHCALPLHRRFGVAATAR
                     ASFALYNTPDEVERLVAGVRRAVDFFGRE"
     gene            complement(3190..3957)
                     /gene="sufC"
                     /locus_tag="VFI55_13580"
     CDS             complement(3190..3957)
                     /gene="sufC"
                     /locus_tag="VFI55_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET7075097.1"
                     /translation="MTTLEIKDLHVSIETPDAAEIPILNGVDLTVKSGETHALMGPNG
                     SGKSTLSYAIAGHPKYRVTSGSITLDDEDVLAMSVDERARAGIFLAMQYPVEVPGVSV
                     SNFLRSAATAIRGEAPKLRHWVKEVKGAMASLEIDAAFAERNVNEGFSGGEKKRHEIL
                     QLELLKPKIAILDETDSGLDVDALRVVSEGVNRYAESEHGGVLLITHYTRILRYIHPE
                     YVHVFVGGRIAESGGSELADELEQNGYVRFTQAAAGA"
     gene            complement(3954..5144)
                     /gene="sufD"
                     /locus_tag="VFI55_13585"
     CDS             complement(3954..5144)
                     /gene="sufD"
                     /locus_tag="VFI55_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET7075098.1"
                     /translation="MTGLTEAVEGSTLAAANKGELFASFDVDAFEVPHGRDELWRFTP
                     LRRLRGLHDGSARATGRAQITVSERPGIHTETVRRGDDRLGQGGVPADRVAAQAFSSF
                     NSATLITVGRNTQIAEPVNVAVTGPGEGAVAYGHLQIRVEELGEAVVVIDQRGSGTYA
                     DNVEFVVDDAARLTVVWIADWADDMVHVSAHHARLGKDAVLRHVTVTLGGEVVRMSAN
                     VRFAAPGGDAELLGLYFADDGQHLESRLLVDHARPDCKSNVLYKGALQGDPASTLPDA
                     HTVWVGDVLIRAEATGTDTFEVNRNLVLTDGARADSVPNLEIETGEIVGAGHASATGR
                     FDDEQLFYLRSRGIPEAQARRLVIRGFFSEIISKIAVPELRERLTIAIEHELAITESR
                     ATAS"
     gene            complement(5144..6595)
                     /gene="sufB"
                     /locus_tag="VFI55_13590"
     CDS             complement(5144..6595)
                     /gene="sufB"
                     /locus_tag="VFI55_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET7075099.1"
                     /translation="MTLTPEATKSQAVEPLTQQEAIASMGTYGYGWADSDVAGASAQR
                     GLSEAVVRDISGKKSEQEWMLQTRLKALRIFERKPMPTWGSNLEGIDFDNIKYFVRSS
                     EKQAATWDDLPADIKNTYDRLGIPEAEKQRLVSGVAAQYESEVVYHSIREDLEKLGVI
                     FLDTDTALREHPDIFKQYFGTVIPAGDNKFSALNTAVWSGGSFIYVPPGVHVDIPLQA
                     YFRINTENMGQFERTLIIADEGSYVHYVEGCTAPIYKSDSLHSAVVEIIVKPHARVRY
                     TTIQNWSNNVYNLVTKRARAEAGATMEWVDGNIGSKVTMKYPAVWMTGEHAKGEVLSV
                     AFAGQDQHQDTGAKMLHLAPNTSSNIVSKSVARGGGRTSYRGLVQVNKGAHGSRSSVK
                     CDALLVDTISRSDTYPYVDIREDDVTMGHEATVSKVSENQLFYLMSRGLTEDEAMAMV
                     VRGFVEPIAKELPMEYALELNRLIELQMEGAVG"
     gene            complement(6592..7287)
                     /locus_tag="VFI55_13595"
     CDS             complement(6592..7287)
                     /locus_tag="VFI55_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7075100.1"
                     /translation="MPDGHTRRAIVRLLLESGSITAGEIGDRLGLAAAGVRRHLDALI
                     EAGDAESTAAAAWQQVGRGRPAKRFRLTPAGRAKLGHSYDDLASAAMRQLREIGGEDA
                     VRTFARRRIDAILAGVAGAEGTEDADIEAAAARVAGALTKAGYVATTTRVGGPIHGVQ
                     ICQHHCPVSHVAEEFPELCEAEQQAMAEVIGTHVQRLATIVNGNCACTTHVPLTPAPS
                     PRRDTTSSEGASV"
BASE COUNT         1214 a         2560 c         2435 g         1288 t
ORIGIN      
        1 ggcgccaaga aggacacggc gttctatcag ggcaaggtcg cgaccgcggc gttcttcgcc
       61 aggaacatgc tgccgaagct gaccgcgcag cgccgggtcc tcgaggctct cgacgacgac
      121 gtgatgcgca tttccgaaag cgcgttctag acggccgaat acacattcca agacacatag
      181 gtcgggcaca gctccagcgg gtacaaccgg tcgtcgggcc atccgacgat tggagaaatg
      241 ttgttatccg tcagctctca catgccacgg ctcacccgtt tcgcggtgct cgcggcgacc
      301 ggggccgcca cgctgtccat cgcggcgtgc ggctcgtcga ccacatcgag tccgacatca
      361 agtcccacgg cgccgagttc caccacgagc cctccagggg cctcagcgac cacgtcgccg
      421 ccggcgaacg gccaagcgca cgtcggcggg ctgatcgcgt ccgtgtcggg caacgcaatt
      481 caggtcaccc agcgggacaa cggcagcgcg acggtggact tcaccccgtc gaccaaggtc
      541 accgaggtca ctccggccgg gctgaccgac gtcaccacgg gcagctgcgt caccgtgcgg
      601 cccagccgcg acgagtctca gggcggtcaa tcgatcaccg ctggatctgt gcgcgtcagc
      661 cccgccgtcg acggcaaatg cccgcagggc acgcaggcca ggccgggttc cgggccgggc
      721 tcgaccccac cggcgccttc cggctcaccg acgaaggcgc cggggaagcg accggcggtg
      781 cagggtgcgg tagcttccgt cgcgggtaac acgatcaacg tgacgaccac cgatgcgagc
      841 ggcaacgtct cgcagacggc cgtcacggtc aacgacaaga ccaggtacac caagcaggcg
      901 tcggccacca gcgaagcgat cagcacgggc aaatgccttg cggcgcgcgg aaccaaggac
      961 ggtagcggca cgctgcaggc gacggacgtc gacctgcgac cggccaacga cggcaactgc
     1021 ggcggcatgg gcagacagcc gcacggccag aggtgacggc tgtctaacga ggcggggcgc
     1081 cgatcagacc gtgaagccga gggcgcgcaa ttgttcgcgc ccgtcctcgg tgatcttgtc
     1141 cgggccccac ggcgggttcc agacccagtt gatccgcaga tcattgacca ggccgctgcc
     1201 gaccagggca ctgcgcgact ggtcctcgat gacgtcggtc agcgggcagg ccgccgaggt
     1261 caacgtcatg tcgatgagcg cgacggtgcc ttcgtcgccc ttttccacgt tcaggccgta
     1321 gaccagcccc agatcgacga cgttgatccc cagttccggg tcgacgacgt cgcgcatcgc
     1381 ctcctcgaga tcgaagcgca gctcctcatc cggtgcgctg gtttcactca ttcctgacct
     1441 cctcgaaggc ttggctcgcc tgatgtggga gcgtcttttt ccgcgctggc ctgggccagc
     1501 gcatccttga acgccatcca acccagcaac gcgcatttca ctcgcgccgg gtatttggcc
     1561 actccggcga acgcgacccc gtcgcccagc acctcctcgt cgccgtcgac ggtgccgcgc
     1621 gaggacacca tctcggtgaa cgcggtgatg gtcttgagcg cctcgccgac gctctggccg
     1681 atcacctgct gggtgagcac cgaggtcgcc gcctggctga tcgaacagcc ttgcccgtca
     1741 taggagacgt cggtgacggt ttcgccgtcg tcggacaacg cgacccgaag ggtcacttcg
     1801 tcaccgcaga tcgggttgac gtggtacacc tgcgcgccga acggttcccg cagcccgcgg
     1861 tgctggggat gtttgtagtg atcgaggatc acgtcctggt agatctgctc aaggcgcaac
     1921 gtcattctct cccaaagaaa tccaccgcgc gtcgcacgcc ggccaccaga cgctcgacct
     1981 cgtcgggcgt gttgtacagc gcaaacgacg cccgtgcggt ggcggccacg ccgaacctgc
     2041 ggtgcagcgg cagcgcgcag tgatgcccga cccgcaccgc gatgccgtcg tcgtcgagca
     2101 cctgccccac gtcgtgcgcg tgcaccccgt cgacgacgaa cgccactggg gaaccgcggt
     2161 tttccatcga cgtcggaccg acgatgcgta cgccgtcgat gccggacagg ccagcaatgg
     2221 cagcggccac cagctctcgt tcgtgggctt cgacggcgtc catgccgatg gcgccgagat
     2281 agcgtgccgc ggcggccaat ccgaccacct gcgaggtcat cggcgtgccg gcttcgaatc
     2341 gttgcggcgc cggggcgtac gtggtggcct ccatggtcac cgtctcgatc atcgacccac
     2401 ctgtgaggaa cggcggcatt tcagaaagca gctcgtggcg cccgtagagc acaccgatcc
     2461 cgttggggcc caacatctta tgtccggaga acgcggcgaa atcgacgtcg agcgcgtgga
     2521 agtcgacggg ctgatgcggc accgactggc aggcgtccag cacggtcaag gcgcccacgg
     2581 cgttggctcg ggcgaccagc tcggccaccg gtgccaccgc cccggtcacg ttcgaatgat
     2641 ggctgaacgc aacgaccttg acgcgttcgt cgagttgcag cgagtccagg tctatgcgcc
     2701 cggcttcttt gtcaccgtcg gtgacgccat accagcgcaa cgtggccccg gtacggcggg
     2761 ccagctcctg ccacggcact aggttggcat ggtgttcgag ctcggtggtg acgatgacgt
     2821 caccgggacc gacagctcga ccgaaacggt tgtcccccag cacatatgac accaggttga
     2881 gcgactcggt ggcgttcttg gtgaacacca attcgtcggg gtcggcgccg acaaacgccg
     2941 cgacgtcggc acggccctgc tcgtagccgt cggttgcctc ctccatcagc tggtgcgcac
     3001 cacgatgcac cgcaccgttg gaggtcagca gaaattcccg ctcggcgtcg agcacctgca
     3061 gcggccgttg ggaggtcgcg ccggaatcca ggtatgccaa ctggtttccc ccgcgcatga
     3121 tccgcttcag gatggggaaa tcggcacgga tcgccgcgag gtccaggtgc ggagccgatg
     3181 cgttcatgat caggcccctg cggccgcttg agtgaagcgc acgtaaccgt tctgctcgag
     3241 ttcgtcggcc agctccgagc caccggattc ggcgatgcgg ccgccgacga agacgtgcac
     3301 gtattcggga tggatgtagc gcaggatccg ggtgtagtgc gtgatcaaca agacgccgcc
     3361 gtgctctgat tcggcgtagc ggttgacgcc ttcgctgacc acccgcagcg cgtccacgtc
     3421 cagcccggag tcggtttcgt cgaggatcgc gatcttgggc ttgagcagct cgagctgcag
     3481 gatctcgtgg cgcttcttct ccccgccgga gaaaccctcg ttgacgttac gctcggcgaa
     3541 ggctgcgtca atctcgagcg aagccatcgc ccccttgacc tctttgaccc agtgccgcag
     3601 cttcggggcc tcgccgcgga tcgcggttgc cgccgagcgc aggaagttcg acaccgagac
     3661 gccgggcacc tcgacgggat actgcatggc cagaaatatt ccggcccgcg cacgctcgtc
     3721 gacgctcatc gccagcacgt cctcgtcgtc cagcgtgatc gaccccgacg tcacccggta
     3781 cttggggtga cccgcaatgg catacgacag cgtcgacttg ccggacccgt tgggccccat
     3841 caatgcgtgt gtctcaccgg atttcaccgt caagtcgacg ccgttgagga tcgggatctc
     3901 ggcggcgtcg ggggtttcga tactgacgtg caggtctttg atttccaggg tcgtcatgaa
     3961 gctgttgctc tcgattcggt aatggccagt tcgtgttcga tggcgatggt gaggcgttcc
     4021 cgcagctcgg gcaccgcgat cttggagatg atctcgctga agaagccgcg gatcaccagc
     4081 cggcgggcct gcgcttccgg gatgccgcgg gagcgcaggt aaaacagttg ctcgtcgtcg
     4141 aaacgccctg tggcactggc gtgcccggcg ccgacgatct cgcccgtctc gatctccaga
     4201 ttgggcaccg agtcagcacg cgccccgtcg gtgagcacca ggttgcggtt cacctcgaag
     4261 gtgtcggtgc ccgtggcctc ggcccggatc agtacgtcgc ccacccacac ggtgtgcgcg
     4321 tcgggcagcg tagaggccgg atctccttgc agcgcaccct tatacaacac gttcgacttg
     4381 cagtcgggcc gggcgtggtc gaccagcagc cgcgactcga ggtgctggcc gtcgtcggcg
     4441 aagtacagcc ccagcagctc ggcgtccccg ccgggcgcag cgaaccgcac gttggccgac
     4501 atccgaacca cctcgccgcc gagcgtcacg gtgacatgcc gcagcaccgc gtccttaccc
     4561 agcctggcgt ggtgggcgct gacgtggacc atgtcgtcgg cccagtcggc gatccacacg
     4621 acagtgagcc gggcggcgtc gtcaacgacg aactcgacgt tgtcggcgta ggttccgctc
     4681 ccccgctggt cgatcaccac gaccgcctcg ccgagctctt ccacccggat ctgcagatgc
     4741 ccgtaggcca ccgcgccctc gcccggcccg gtcacggcga cgttcaccgg ctcggcgatc
     4801 tgcgtgttgc gcccgacggt gatcagcgtc gcggagttga acgacgaaaa tgcctgggcc
     4861 gcaacacggt cagcgggcac gccgccctgc cccagccggt cgtcgccgcg gcgcacggtt
     4921 tcggtgtgga tgccgggccg ctcgctgacg gtgatctgcg cccgaccggt ggcccgtgcg
     4981 gagccgtcgt gcaggccgcg caaccgtcgc agcggggtga accgccaaag ctcgtcgcgg
     5041 ccgtgcggaa cttcgaaggc gtccacgtcg aaggacgcga agagctcacc cttgttggca
     5101 gcggcgaggg tcgaaccctc aaccgcttca gtcaatcccg tcactagccg accgcacctt
     5161 ccatctgcag ctcgatcagc cggttgagct ccagcgcgta ctccatcgga agctccttgg
     5221 cgatcggctc gacgaagccg cgcaccacca tcgccatggc ctcgtcttcg gtcagcccgc
     5281 ggctcatcag gtagaacagc tggttctcgc tgaccttcga cacggtggcc tcgtggccca
     5341 tcgtgacgtc gtcctcacgg atgtccacgt aggggtaggt gtcgctgcgg ctgatcgtat
     5401 cgaccagcag cgcatcgcat ttcacgctcg agcgcgaccc gtgcgcaccc ttgttcacct
     5461 gcaccaggcc gcggtaggag gtgcggccac cgccgcgtgc caccgacttg gacacgatgt
     5521 tgctcgacgt gttcggtgcc aggtgcagca tcttggcgcc ggtgtcctgg tgctggtctt
     5581 ggccggcgaa agccaccgac aacacctcac ccttggcgtg ctcgccggtc atccagaccg
     5641 ccgggtactt catggtcacc ttcgacccga tgttgccgtc gacccattcc atggtggcgc
     5701 cggcctcggc gcgggcccgc ttggtgacca ggttgtagac gttgttcgac cagttctgga
     5761 tggtggtgta gcgaacgcgc gcatgtggtt tcacgatgat ctccaccacc gcggagtgca
     5821 gcgaatccga tttgtagatc ggcgcggtac acccttcgac gtagtgcaca taggagcctt
     5881 cgtcggcgat gatcagcgtt cgctcgaact ggcccatgtt ctcggtgttg atccggaagt
     5941 aggcctgcag cgggatatcg acgtgcacac cgggcgggac gtagatgaag ctgccgcccg
     6001 accacactgc agtgttgagg gcggagaact tgttgtcccc ggccgggatc acggtgccga
     6061 agtactgctt gaagatgtcc ggatgttcac gcaacgccgt gtcggtgtcc aggaagatca
     6121 cgcccagctt ctccagatcc tcacggatgg agtggtagac cacctccgac tcgtattggg
     6181 cggcgacacc ggagaccagc cgttgcttct cggcttccgg gatgcccaac cggtcgtagg
     6241 tgttcttgat gtccgcgggc agatcgtccc aggtcgcggc ctgcttctcg ctggagcgca
     6301 cgaagtactt gatgttgtcg aagtcgatgc cctcgaggtt ggagccccac gtcggcatgg
     6361 gcttgcgctc gaagatgcgc aacgccttca gccgggtctg cagcatccat tcctgctcgc
     6421 tcttcttccc ggagatgtcg cggacgaccg cctcggacag tccgcgctgt gcgctggcgc
     6481 ccgcgacgtc ggagtccgcc cagccatagc cgtacgtacc catcgaggca atcgcctcct
     6541 gctgggtcaa cggctcgacg gcctgcgact tagtcgcctc gggtgtgagt gtcatacgga
     6601 cgctccttca ctgctggtgg tatcacggcg cgggctgggc gccggcgtaa gaggcacgtg
     6661 ggtggtgcag gcgcaattgc cgttgacgat ggtcgccaac cgctgcacgt gggtgccgat
     6721 cacctcggcc atggcctgct gctcggcttc acacagctcc ggaaattcct cggccacgtg
     6781 cgataccgga cagtggtgct ggcagatctg cactccgtgg atcggcccgc cgacgcgcgt
     6841 ggtggtggcg acgtagccgg ctttggtcag cgcaccggcg acccgcgcgg cggccgcctc
     6901 gatatcggcg tcctcggtgc cttcggcgcc cgccacgccg gccaggatcg cgtcgatccg
     6961 tcgccgggcg aaggtccgga cggcgtcctc accgccgatc tcccgcagct ggcgcattgc
     7021 cgccgacgcc aagtcgtcgt aggagtggcc gagcttcgcg cggccggccg gggtcagccg
     7081 gaaccgcttg gcgggccgcc cgcggcccac ctgctgccac gccgcggcgg ccgtggactc
     7141 ggcgtcgccc gcctcgatca gcgcgtcgag atggcgccgt acgcccgcgg ccgccaggcc
     7201 gagccggtca ccgatctcac ctgcggtgat cgatccggac tccagcaaga ggcgcacgat
     7261 ggcgcggcgg gtgtgaccat ccggcaccgc cgtgccgccc gcaccgccgg gagcctcaga
     7321 agtgtgagga acctcatcga gggcggttcg gattttcaca acacaagtgt gacgcaattc
     7381 ctggcgcggg tccagcgagg gtgccctatc ttcgcgttgc accggtcaca ccgcttgcgc
     7441 agccacccgc tccacaacac gccgcttgcg cggcctgtct cgtcgccggg gctaggc
//
LOCUS       DASSGQ010000309         2480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_387344,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000309 DASSGQ010000000
VERSION     DASSGQ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387344"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1517
                     /locus_tag="VFI55_13600"
     CDS             <1..1517
                     /locus_tag="VFI55_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7075101.1"
                     /translation="TWPDRVAVVDAVGTHTYAELDRLVDRAAGGFAALGIAPADRVLL
                     QLPNSCRFAVALFGLLRAGAIPVLCLPGHRLAELSRFAAGSDAVGLVIADTAGGFDYR
                     AMAEQLVALHPALRHVIVDGLVDGATDGDPGRFIPWSGLPAGATPHVVTDTASPALLL
                     VSGGTTGTPKLIPRTHDDYVYNATASAQLCRLTGDDVYLIALPAAHNFPLACPGLLGA
                     MTVGATVVFGTDPSPEAGFAAIARHRVTVTALVPALAKLWAQACDWEPVTPKSLRILQ
                     VGGARLEPDDARLVRATLTPGLQQVFGMAEGLLNYTRLDDPAELVENTQGRPLCADDE
                     LRIVDGAGRPVAPGEEGELLVRGPYTLNGYFRAERDNERCFDPDGFYRSGDLVRRRDD
                     GYLVVTGRVKDVICRAGETIAAADLEEQIMSHSAVFSAAAVPLPDPYLGEKICAAVVF
                     VGEPIGLAELNAHLDERGVAAHARPDMLVAMAALPTTPIGKIDKKAIVRELVGS"
     gene            complement(1587..2063)
                     /locus_tag="VFI55_13605"
     CDS             complement(1587..2063)
                     /locus_tag="VFI55_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015292931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ricin-type beta-trefoil lectin domain protein"
                     /protein_id="HET7075102.1"
                     /translation="MQVPRLMGGARLALSVIGAVFSVAVLGAGVARADGPVQLRNRAG
                     DFCLDRPDGSIYTKTVINPCNGSLSQRWNLLGTGQIQSAAFPAMCLSKENRDYWAFVL
                     GCLNPGYEVWAIQPNGQIQQQFGGFCLTPVGDLNPGTWVSTNWCNGDAPEQWDVVP"
     gene            complement(2249..>2480)
                     /locus_tag="VFI55_13610"
     CDS             complement(2249..>2480)
                     /locus_tag="VFI55_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HET7075103.1"
                     /translation="LRLDEVPRGLYSGAVVMVSADGGLDAALTLRAAYECDGKTWLRA
                     GAGIIEESDPDREFEETCEKLSTLAPYLVARQ"
BASE COUNT          431 a          857 c          773 g          419 t
ORIGIN      
        1 cgacctggcc cgaccgagtg gccgtcgtgg acgcggtcgg cacccacacc tacgccgagc
       61 tggaccgact cgtcgaccga gctgccggcg gcttcgccgc gctcgggatc gcaccggccg
      121 accgagtgct gctgcagctg cccaattcct gccggttcgc cgtggctctg ttcgggttgc
      181 tgcgcgccgg cgccatcccg gtgctgtgcc tgccgggcca tcgattggca gaactcagtc
      241 gcttcgcggc gggcagcgac gcggtcggcc tggttatcgc cgacaccgcg ggcggtttcg
      301 attaccgggc gatggccgaa cagttggtgg cgctgcatcc agcgcttcgc catgtcattg
      361 tcgacggcct tgtcgatggc gctaccgacg gcgaccccgg ccgattcatc ccatggtcgg
      421 gattgcccgc aggcgccacg ccccacgttg tgaccgacac cgcctccccc gcattgctgc
      481 tcgtgtcggg cggcaccacg ggtacgccga agctcattcc ccgcacccac gacgactacg
      541 tctacaacgc caccgcgagc gcgcaactat gtcggctgac cggcgatgac gtttatctca
      601 ttgcgctgcc ggccgcacac aatttcccgc tggcctgtcc gggcctactt ggcgcgatga
      661 cggtgggcgc caccgtcgtg ttcgggaccg atcccagccc cgaggccggc tttgccgcca
      721 tcgcgcgtca ccgcgtgacg gtcacggctt tggtgccggc actggccaag ctgtgggcgc
      781 aggcttgtga ctgggagccc gtgacaccaa agtcgttgcg gatcttgcag gttggcggcg
      841 ccagactgga gccggacgat gctcgcctgg tgcgcgccac gctgaccccg ggcctgcaac
      901 aggtgttcgg catggccgag gggctgctga actacacgcg cctggacgat cctgccgaac
      961 tcgtcgagaa cacccagggg cggccgttgt gtgccgacga cgaacttcgc atcgtcgacg
     1021 gcgcgggccg gccggtggcg ccgggcgaag agggcgagct gctggtgcgc ggaccgtata
     1081 cgctcaacgg ctacttccgt gccgagcgcg acaacgagcg ctgcttcgac cctgacggct
     1141 tctaccgcag cggcgacctg gtccgccgca gagacgacgg ctacctcgtg gtcaccggcc
     1201 gggtcaaaga cgtcatctgc cgtgccggtg aaaccatcgc cgcggccgac ctcgaagagc
     1261 agataatgag ccactccgcg gtcttttcgg ccgcggcggt tccgctgccc gacccctacc
     1321 tgggcgaaaa gatttgcgct gcggttgttt tcgtcggaga accgatcgga ctcgcggagc
     1381 tgaacgcgca tctcgacgaa cgcggagtgg ccgcgcacgc ccgacccgac atgctggtcg
     1441 cgatggcagc actgcccact acaccgatcg gcaagatcga caaaaaggcg atcgtccgcg
     1501 agctcgtcgg gtcgtgaccc ccgcgagcgt aacgccactg cgaaaaacgg cctccgaatt
     1561 cgcagtggcg ttacgctcgg gacgggtcac ggcaccacat cccattgttc cggcgcgtcg
     1621 ccgttgcacc agttggtgct cacccaggtc ccgggattca ggtcgccgac cggggtaagg
     1681 caaaagccgc caaactgttg ctggatctgg ccgttgggct ggatagccca aacctcgtac
     1741 ccaggattca gacagccaag gacgaatgcc caataatccc tgttttcctt agacagacac
     1801 atcgcgggga aagccgcgct ctgaatctgc ccggtaccca gcaaattcca gcgctgggat
     1861 agcgacccgt tgcaggggtt gatcaccgtc ttggtgtaga tgctcccgtc gggacggtcc
     1921 agacaaaaat cgcctgccct gtttctcaac tggacggggc cgtcggcacg tgccacacca
     1981 gcgcccagca cggcgacact gaacactgcg ccgattaccg acagggccaa ccgcgcaccg
     2041 cccatcaacc gcggcacctg catgtgagtt acatcctctc aactcggtcg agtgcgccac
     2101 cgcctcaaac ggttgccacc agcaacaaga ttgctgcccc atgctgcgct tccgacattc
     2161 aaacaagctt ggtgaatgaa atgaatccgc taggaaagta ccgtctgggg ttgccccacc
     2221 ccaccggcgc ggcgcctcgc gtcagaagct attgccgcgc aaccagatac ggcgcaagag
     2281 tggaaagctt ttcgcaggtc tcctcgaatt cgcgatcggg gtccgactct tcgatgattc
     2341 cggcaccggc ccgcaaccac gtcttcccgt cgcattcata ggccgcgcgc agcgtcagcg
     2401 cggcgtccag cccaccgtcg gcggaaacca tcacgaccgc accggaatag agtccgcggg
     2461 ggacttcgtc gaggcgcagg
//
LOCUS       DASSGQ010000310        21163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_445374,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000310 DASSGQ010000000
VERSION     DASSGQ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 21163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21163
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445374"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFI55_13615"
     CDS             <1..759
                     /locus_tag="VFI55_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075104.1"
                     /translation="DVDPDLAALYQPMLARETNRNLGKPLAIAVDTIERLHATAEREG
                     ARLHLLTERDDLVGAAAILAAADRIRYLTPRLHAEMVSELRWPGEGNPDTGIDVRSLE
                     LEPDDLAMVGILRRPDVMAQLARWNVGSVLGEDTGRRIAASSALAVISVAGGSLADYA
                     RGGSAVEAVWIIAQQHGLAIQPISPVFLYARDAEDLAELSTAFADDLGELEQKFRELV
                     RIPASASPALMLRVAVAAPASVRSRRTIERVRLL"
     gene            786..2723
                     /locus_tag="VFI55_13620"
     CDS             786..2723
                     /locus_tag="VFI55_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7075105.1"
                     /translation="MPGSLDDLVTAAAAQLMAATAASHVAVSQRVLADLVSHFGVDSG
                     FLRHNDHAIRATVLVAEWPPRENVPDPDPIGVVYFADADSVFGLIENHKVPFVLRPEA
                     ANADYQQNIEEGRGVALTSLAAVPLLSGDLTIGTLGFIKFGDREWLPEELNALQAIAA
                     LLAQLQARIVAEERVRYLADHDDLTGLLNRRALIAHLDQRLAKGKKGHVAVLFLDLDR
                     FKVVNEHLGQHAGDRFIRGFAEFLREAADPSSVTSRLGGDEFVVVPAAPMDIKSAVTF
                     AHWLQDRVDKQVVIDGEKLSRSVSIGVASGIPGRDSTSDLLRRADQAARAAKNSGGAK
                     VAAFSAEMSERDMIRNDIELDLEGTGSSGSSALVLHYLPEFGIDTGRILGTEALIRWQ
                     HPTLGLLMPDSFIGVVESINLAAKLGRLVMRSACSQFGFWRSRGLGLDIVMRINVSPV
                     QLVAGGIVETVAAILDEFAIDPGAICLEITERVVVQEIDTTRRTLARLKDVGVQIAID
                     DFGTGYSSLTYLKSLPVDTIKIDRAFVRDLGTNGRDLAIVQAIVDLAHALGLGVVAEG
                     VETATAAKALLDLGCNRAQGFLLSRPLDSDAMESLLAKRVVPMDFSSIEDLPAEDSSN
                     RRGMGKSSGRSSTRAGMQRLR"
     gene            complement(2811..3560)
                     /locus_tag="VFI55_13625"
     CDS             complement(2811..3560)
                     /locus_tag="VFI55_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075106.1"
                     /translation="MTVVSLGAMSVDRFRLDEGLSWHDPDIDCTLLVSQPSVDPDLWG
                     EYAAGAQRSYRKHGVECALDVDALRTGADTEMFFAVIDRAGKMVAGVRAMGPLGSAED
                     SHALVEWAGQPGEGAVRNMINDRVPFGVLEMKSAWATDDPERSPSLTQAVARSAFHMM
                     AILDIQFCMATAAAYALNRWRSSGGVVASIPATPYPSERYQTKMMWWNRRDFVNHAEP
                     EQVAKILQETAQLTQQFYRQSGNEARLFDLA"
     gene            4449..4700
                     /locus_tag="VFI55_13630"
     CDS             4449..4700
                     /locus_tag="VFI55_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009030028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075107.1"
                     /translation="MSTKYYLQKVPVESVQPGFSLAIHRDGDYRLFQVECTQMSHRSG
                     QSVQFKLISEPVSGGDPWVLECEAGTPVVRILGVCKAAC"
     gene            5020..6021
                     /locus_tag="VFI55_13635"
     CDS             5020..6021
                     /locus_tag="VFI55_13635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7075108.1"
                     /translation="MRGITVAELTGARAEELATMDIFEGCSTQDLEPLAGCLQPLRAA
                     ADQVLMQQGEQAVSFLLISSGTAEIKHVGSDGEVAVGQTPPGTIVGEIALLRDKPRIA
                     TVTTAEALTGWIGNNDAFALMVQIPGVMPRLLRTVRQRLAGFITPIPIQARDGTELML
                     RPVLPGDDERTIQGHIQFSSETLYRRFMTARVPSPALLHYLSEVDYVDHFVWVVTDSS
                     DPVADARFVRDETDPKSAEIAFTVADAYQGRGIGSFLISALSIAAKVDGVERFSARML
                     SDNAAMRAIMDHYDAVWQREDVGVITTVIDVPDRLAFGPEMAGKIRRVARQVIEAVG"
     gene            6085..6783
                     /locus_tag="VFI55_13640"
     CDS             6085..6783
                     /locus_tag="VFI55_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5642 family protein"
                     /protein_id="HET7075109.1"
                     /translation="MRPFRIGAVATPVVTLLVAGCAHAPAPMPSAKPTTSQAAGGNAV
                     NPANIKRLVRELPPNYEVTAGIPSGASPRVIWSLEPDASDIRVNPPQCATLADPGDGR
                     DQSAQGVSGSGSGGIVDAVVVALPGPAELDHDLVAACGRWTMIDGHTTVSIHLTDPPH
                     LDGVETLGMVADTKSSVESGTEIDSRAYTFTAYLGNYYAFTTLTTDPGSALPTLSPQF
                     AADLLVKTVSTLRG"
     gene            6811..7467
                     /locus_tag="VFI55_13645"
     CDS             6811..7467
                     /locus_tag="VFI55_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5642 family protein"
                     /protein_id="HET7075110.1"
                     /translation="MFKVLLAAASVCALAGCSSGSDASSAKADISKVADVKSSLGPEY
                     RVSDISERAIDPKLLSARKLPDGLTFDPANCAKEAVGPDMPPDLQGNMAAVSAEGNGN
                     RFVVIAVETSKPVPFNDPGKDCAKVAFAGAQVRGGIEVIDAPHIDGTQTMGVHRVLQA
                     VLDGGTRTGELYDYSAQFGDYQVIVTANPLVVPDQPVAKVDTQRARDLLVKAVAAIRA
                     "
     gene            complement(7464..8072)
                     /locus_tag="VFI55_13650"
     CDS             complement(7464..8072)
                     /locus_tag="VFI55_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HET7075111.1"
                     /translation="MAIAVQGSLFEHTERRQLGDGAFIEVRAGWLTDEPSGSEGLLDA
                     LLSTVPWRAERRQMYERVVDVPRLVSFHDLTVEEPPHPLLGRLRRRLNDIYAGELGEP
                     FTTVGLCCYRDGSDSVAWHGDTIGRSSSQDTMVAIVSLGATRTFALRRRGGGASLRLP
                     QAHGDLLVMGGSCQRTWEHAVPKTSAPTGPRVSIQFRPRDVR"
     gene            complement(8142..8828)
                     /locus_tag="VFI55_13655"
     CDS             complement(8142..8828)
                     /locus_tag="VFI55_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7075112.1"
                     /translation="MSAPRVPRRRPADERSSPERIRAAAMRVVAHHGVDATSLRMIAD
                     AAGVSIGLIQHHFGTKGQLIEAIDGYVLGVVRGAIEPSPLPGPPADPLVEAGLRMVSL
                     IAEHADVVDYLGRALVERDAIGSIIFDGLVGISAAQGDQLIKQGLGRPDLDLVWGVLN
                     PLILRVGAIILRPHIERYLPEPFATRGQLMRWDAAVTKLLRAGQLRAPLDPADAAGPA
                     PKEHGRNNNG"
     gene            complement(8940..9284)
                     /locus_tag="VFI55_13660"
     CDS             complement(8940..9284)
                     /locus_tag="VFI55_13660"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7075113.1"
                     /translation="MPARLLLDRLPVPIWAVHGDTVVYTNDAFAEMIGHDRSHLSAVF
                     ANELIRDVPALDTPVTTLLQQRAGELMALRHADGSILQVVVSRPMLLRADDPVTLVGV
                     QDVTEHIWDKSR"
     gene            9535..10746
                     /gene="arcA"
                     /locus_tag="VFI55_13665"
     CDS             9535..10746
                     /gene="arcA"
                     /locus_tag="VFI55_13665"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HET7075114.1"
                     /translation="MGVVELGTNSEVGALRVVILHRPGAELRRLNPRNNDQLLFDGLP
                     WVARAQEEHDQFAGLLRSRGVEVLLLSDLLTEALHHSGAARIQGVAAAVDARRLGAPL
                     AQELSAYLRTLEPVRLAEVLMAGMTFNELPSATRSDVSLVLRMHHGADFVIEPLPNLV
                     FTRDSSIWIGPRVVIPTLALRARVREASLTDLIYAHHPRFTGVRRAYESRTAPVEGGD
                     VLLLSSGVVAVGVGERTTPAGAEALARSLFDDDLANTVLAVPIAQRRAQMHLDTVCTM
                     VDTDTVVMYANVVDTLSAFTIEHTPDGVTISDETPFLEAAAKAMGIDKLHVIDTGLDP
                     VIAEREQWDDGNNTLALAPGIVVAYERNVQTNTRLQDAGIEVLTIAGSELSTGRGGPR
                     CMSCPVARDLI"
     gene            complement(10801..11649)
                     /locus_tag="VFI55_13670"
     CDS             complement(10801..11649)
                     /locus_tag="VFI55_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075115.1"
                     /translation="MTRVFLGSEAVARGTLTRAQLRWNYRRIHRNVYLPKNAPRSLWS
                     NIYAAWLWSGRRGIIAGRAAAALHGAKWVDEFTPVEVVGPFNHPAPGIIVRRERIAVA
                     DLVEMRGLLGTNPARTAFDLARHLPRAVAVTHLDALSAATGVTAADVTPLIERHRGAR
                     GVRQCRNALSLMDSGAQSPKESWLRLALIDAGLPRPRTQIRVSDGHLVAYLDMGWEMQ
                     MVALEYDGDQHRTDRRQYVSDIRRAEMFDRLGWHVIRVINEDRPNVIVQRARDALVLL
                     PPPRLP"
     gene            complement(11837..13414)
                     /locus_tag="VFI55_13675"
     CDS             complement(11837..13414)
                     /locus_tag="VFI55_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate-mannose--protein
                     mannosyltransferase"
                     /protein_id="HET7075116.1"
                     /translation="MTAPPGESPVAVQDRVERVVPVVSPGPLVPVADFGPTDHIRGWI
                     TTGVITVLATVTRFLNVGSPTDAGTPVFDEKHYAPQAWQVLHNHGVEDNPGFGLVVHP
                     PVGKQLIAIGEAIFGYNGVGWRFTGALLGVVMVTLVMRIVRRISRSTLVGAIAGVLLI
                     CDGVSFVTARSALLDGFLTFFAVAAFGALIVDRDQIRERMYLALLEGRSAETVWGPRL
                     GVRWWRFGAGVLLGLACGTKWSGLYFVVFFGAMSLAFDVAARREYQVTRPWLGTVRRD
                     LLPTTYALGLIPVAVYLASYAPWFASETAIDRHEVGLSIGPHSVVPLPDAIRSLWHYT
                     AKALQFHAGLTNSAGNYHPWESKPWSWPMSLRPVLYAIDQQNVPGCGAQSCVKAEMLV
                     GTPAMWWVAAPVLLYATWRMLVRRDWRYAVVWVGYCAGWLPWFADIDRQMYFFYAATM
                     APFLVMAIALICGDILYPAGRALGQNSERRTLGLIAVSCYTALVVTNFAWLFPVLTGL
                     PISQQTWNMEIWLPSWR"
     gene            13439..14275
                     /gene="rsmI"
                     /locus_tag="VFI55_13680"
     CDS             13439..14275
                     /gene="rsmI"
                     /locus_tag="VFI55_13680"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET7075117.1"
                     /translation="MTAGLLLLGATPLGQPSDASRRLIDALTHADVVAAEDTRRVRIL
                     AKALDAPITGRVVSLFDRVESARVPALVDAIKAGATVLLVSDAGMPLISDPGYRLVAA
                     CVDAGLPVQCLPGPSAVTTALAVSGLPSERFCFEGFAPRKKSARQAWLASLADERRTC
                     VFFESPRRLDACLHDAVEQLGGARRAAVCRELTKVHEEIVRGSLDELARWAADGVLGE
                     ITVVLAGATPRADVPALLAEVEELVAAGARVKDACSQVAAGHPEVRSSQLYDAILQLR
                     DE"
     gene            complement(14301..14987)
                     /locus_tag="VFI55_13685"
     CDS             complement(14301..14987)
                     /locus_tag="VFI55_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075118.1"
                     /translation="MGGPGLGVGGVPGGGVSGAGLPDVGGVGGLPDLGLPGGIDPYAL
                     AGQVPGLGGLAGLAAIPNSAAVALSVVQGVVGVGGSLVGVGATAISTVEMGAIAYTYL
                     HQAGLVPAVSLPSLPGISIPGVGLPAAAAAIPGIAATLPAVGAAIPAVGLPAVGLPAA
                     PALPAVGLPAAPALPAVGLPAAPALPALPLAPPALPAPPPLPALPAPPAIPHPRLCSP
                     GFGPIGVCTP"
     gene            15061..15336
                     /locus_tag="VFI55_13690"
     CDS             15061..15336
                     /locus_tag="VFI55_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075119.1"
                     /translation="MAAVEKGVGGGAGAAAGADGLGVSGIRTDATFAHTAPPLPQVAA
                     VGSGVLTEVSGIAASAPPITADAAAPTTAILMTDPSSRRPTTGAGDG"
     gene            complement(15416..16669)
                     /locus_tag="VFI55_13695"
     CDS             complement(15416..16669)
                     /locus_tag="VFI55_13695"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HET7075120.1"
                     /translation="MRIDRLGDLGAAPRVLRAVGDATSRLDLPPPAALTGDWFGALAV
                     IAPSVSVQPVSIDDAFAVPPDSQASYARAVGGGWVGYLSYPDPGADGRPNRIPEAAGG
                     WTDCVLRRDPDGQWWYESLSGAPMPDWLTAALAARPAPPRDCRIDWDAADRAAHRDGV
                     LACLEAIRAGEVYQACVCTQFAGTVSGAPLDFFVDGVARTSPARAAFVAGPWGAVASL
                     SPELFLRRRETVVTSSPIKGTLPLDAWPSALRASAKEVAENIMIVDLVRNDLGRVAIV
                     GTVTVPELLVVRRAPGVWHLVSTVSARVPVELPTSALLDATFPPASVTGTPKHRARQL
                     ISQWEHRRRGIYCGTVGLASPIAGCELNVAIRTVEFHADGGAVLGVGGGITADSDPDA
                     EWEECLHKAAPIVGLPCIAAAAVVG"
     gene            complement(16760..17683)
                     /locus_tag="VFI55_13700"
     CDS             complement(16760..17683)
                     /locus_tag="VFI55_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HET7075121.1"
                     /translation="MTPSRGGEHAERFTLLRPLLFTIAYEILGSATEADDVLQDSYLR
                     WAAVDLSGVRDTKSYLAQLVTRQALNALRAGARRREDYVGPWLPEPLLLDDQDPSADV
                     VLAESVSIAMLVLLETLSPDERAVFVLREVFGFDYREIAEAVGKPAPTVRQVAHRARE
                     HVRARRKGGGAAKMSIDPERNAEVTAQFLATAVSGDVESLMVMLAPDATWTADSGGKV
                     SAARRPVVGAARVARAIVGLMRKTQEMWAALRVELVTCNSAPAVLVYDDEHLEMAITV
                     EIVDDKITNFYVMRNPDKLTTLAAARDISRG"
     gene            complement(17680..18885)
                     /locus_tag="VFI55_13705"
     CDS             complement(17680..18885)
                     /locus_tag="VFI55_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7075122.1"
                     /translation="MTEMTTPRTHVVVVGGGYAGTLAANHLRQRADVDVTLVNPRPVF
                     VERIRLHQLVADTGSATAAYDTLLGDGIRLVVDTVERINAADRRVLLESGAELAYDYL
                     IYAVGSMGAVAAKLSRLPEVPGLAEFGHSIADLEGAQRLRYALADLPPTAPITVVGGG
                     LTGIETASELAERGRQVTLVCGGVLGPSLSRRGRRSVARQLRNLGANVLEPVRVSQVR
                     WDAVVLDDGSVLPSAATVWTAGFAVPDLAARSGFRTDAVGRLLTDETMTSIDDDRVVA
                     AGDAAAPSGQPLRMSCQAAGPLGAQAANTVLSRIAGTAPAVLNQAFVGQCISMGRAHA
                     TFQLTRTDDTPVNVVLAGRVAAPIKEAICKGTLWAIRREATKPGSYVWLKGGNRPATV
                     PAGPQVALQ"
     gene            19350..>21163
                     /locus_tag="VFI55_13710"
     CDS             19350..>21163
                     /locus_tag="VFI55_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7075123.1"
                     /translation="MPRSLDLIVTSVASQLMEATGATATQVSERVLAQLVEQFDVDAS
                     FLRHHDHEIRASILVAEWPPRDNVPDPDPLAVAHFTSADPVLAQCEHGRKPVVVRPEP
                     SSRYFQRLVGGSKQVTSPSVAAAPLVSGEMTTGVLGFVKYGARKWKQEELNTLEAVAA
                     LFAQLQARIAAEEKLRYLAEHDDLTGLYNRRALVAHLSERLSAGSPGPVAVLYLDLDR
                     LKPINDYLGHTAGDWFIRVFAQRIRGCAGQSMIARLGGDEFIVIPDQTMSSGAAESFA
                     RRLAGMLCERLAIGGHMITRTVSIGVTVGVPGRDNSTDLLRRADEAVLTAKRAGGNQV
                     AMSTDDMSLKSAFRNDIELHLQGDIDSEALLLHYLPEVDLWTGAIVAAEALVRWQHPI
                     WGLLLPDSFIGVAETTNLAGELGRWVMRTACAEFSRWRSKGIGQNVALRINVSPVQLI
                     TRGFVRSVADTIEEFGIDAGSVCLEITERAVVHDIETTRKTLAELKEVGVQIAIDDFG
                     TGYAVLSHLKSLPVDMLKIDNGFVRDLGTNAGDLAIVRAIIGLAEAFGLQLVAEGVET
                     PAAALTLMQHGCHRAQGFLLSRPVPGDAMEALLAARWMP"
BASE COUNT         3569 a         6970 c         7099 g         3525 t
ORIGIN      
        1 gatgttgatc ccgacctggc cgcgctctac cagccgatgc tggcccgtga aaccaatcgc
       61 aacctgggaa agccgctcgc gatcgccgtg gacacaatcg aacggctgca cgccaccgcc
      121 gagcgggagg gagctcggct gcatctgctg accgagcggg acgacctggt cggagctgcc
      181 gcaatcctgg ctgccgcaga ccgcatcaga tacctgaccc cgcggttgca cgccgaaatg
      241 gtttcggagc tgcgctggcc cggcgaaggt aatcccgaca ccggtatcga cgtgcgcagc
      301 ctcgagctcg aacccgacga tctcgcgatg gtcggcattt tgcggcgccc ggatgtgatg
      361 gcgcagctgg cccggtggaa tgtcgggagc gttttgggcg aggacaccgg tcgccgcatc
      421 gcggccagct ccgcgttggc cgtcatttcg gttgcagggg ggagcttggc cgactatgcg
      481 cgcggcggat ctgccgttga ggcggtatgg atcatcgcac agcagcacgg attggcgatc
      541 caaccgatct caccggtgtt cctgtacgcc cgcgatgccg aagatcttgc cgaattgtcg
      601 acggcgtttg ctgacgacct tggagaacta gagcaaaagt tccgggaact ggtccgcata
      661 cccgccagcg catcacccgc cctcatgctg cgtgtcgctg tcgcggcccc ggcgtcggtg
      721 cggagccgtc gcactattga gcgcgtccgt ttgctgtagc gccgctacag gctaccgtat
      781 tgggaatgcc gggctcgttg gatgatttgg tcaccgcggc ggccgcccaa ttgatggcgg
      841 ccacggccgc tagccacgtg gcggtcagtc aacgggtgct cgccgacttg gtcagccact
      901 tcggtgtgga cagcgggttc ctgcgccaca acgaccacgc catccgcgcg accgttcttg
      961 tcgccgaatg gcccccacgc gagaacgtcc ccgaccccga cccgatcggt gtggtgtatt
     1021 tcgccgacgc cgactcggtc ttcgggctca tcgaaaacca caaggtgccc ttcgttctgc
     1081 ggcccgaagc tgcgaatgcc gactatcagc aaaatatcga ggagggcagg ggcgttgcgc
     1141 tgacttcgct cgcggccgtt ccgctgcttt ccggcgatct caccatcggc accctcggct
     1201 tcatcaagtt cggtgaccgt gaatggctgc cggaggagct caatgcgcta caagcgatcg
     1261 ccgcgctatt ggctcagctg caggcccgca tcgtcgccga agagcgcgtc cgctacctgg
     1321 ctgaccacga tgaccttacc ggcctgctga atcgcagggc gctgatcgcc cacctcgacc
     1381 aacgcctggc caagggtaag aagggtcacg ttgcggtgtt gttcctggat ctggaccgtt
     1441 tcaaggtcgt caacgagcac ctgggtcagc acgccggcga ccgcttcatc aggggtttcg
     1501 ccgagttcct gcgcgaggcc gcggacccgt cgtcggtgac ttcccggctc ggcggtgacg
     1561 agtttgtggt ggtgcctgct gcgccgatgg acatcaaaag cgcggtgacg tttgcgcatt
     1621 ggctccagga ccgggttgac aagcaggtcg tgatcgacgg cgagaagctc agccgttcgg
     1681 tcagcatcgg ggtcgcgagc ggtataccgg gacgcgacag cacttcggac ctcctgcgtc
     1741 gcgctgatca ggccgcaagg gcggccaaga attccggcgg ggccaaagtc gccgcattca
     1801 gcgcggagat gtccgaacgg gacatgatcc gcaacgacat cgaactggat ctggaaggaa
     1861 ccggcagcag cggtagcagc gccttggtgc tgcattacct gccggagttc ggtattgaca
     1921 ccggccggat cctcggcacc gaggcgctta tccgttggca gcacccgacc ctgggactgt
     1981 tgatgcccga ttccttcatc ggggtcgtcg aatccatcaa tctggctgcg aaacttggcc
     2041 gtctcgtcat gcgttcagcc tgctcgcagt tcggattttg gcgctcgcgc ggcctgggcc
     2101 tcgatattgt gatgcgcatc aacgtatcgc ccgtgcagct cgtcgcgggt gggattgtcg
     2161 agacggtggc cgcgatcctc gacgagttcg ccatcgatcc cggcgccata tgtctcgaga
     2221 tcactgaaag agtcgtggtc caggaaatcg acaccacgcg ccgaacgctg gctcgactta
     2281 aagacgttgg cgtacaaatc gcgatcgacg acttcggtac cgggtacagt tcgctgacct
     2341 acctgaagtc actgccggtc gacacgatca agatcgacag agcctttgtc cgtgatctcg
     2401 gcaccaacgg acgcgatctg gcgatcgtgc aggccatcgt cgacttagcg cacgccctgg
     2461 ggctaggggt tgtcgcggag ggcgttgaga cggccacagc tgccaaggcg ctcctcgatt
     2521 tggggtgcaa tcgggcgcag ggctttttgc tgtcccgtcc tttggacagc gacgcgatgg
     2581 agtcgctgct cgccaagcgc gtcgtgccga tggacttctc cagcattgaa gatttgccag
     2641 ccgaggattc gtcgaatagg cgggggatgg gcaagtcttc gggacgaagc tccactcgcg
     2701 cgggaatgca gcggcttcgt tgacgcgacg gcgcgtcctg cggccaagcg ttcacgggca
     2761 tcggtgcgat ccgttcgggc agccgatcga ctggcggttt ggatgcgcaa tcaggccaaa
     2821 tcgaataggc gggcttcgtt cccggactgg cggtagaact gttgcgtcag ttgtgccgtc
     2881 tcctgcagga tcttggcaac ctgctctggt tccgcgtggt tgacgaaatc gcgccggttc
     2941 caccacatca tcttggtttg atagcgctcg ctgggatacg gcgtcgcggg aatgctcgcc
     3001 accacgccgc cggacgaacg ccaccggttc agggcgtagg ccgcggctgt ggccatgcag
     3061 aactggatgt caagaatggc catcatgtgg aaagcgctgc gggcaacggc ctgcgtcagg
     3121 ctgggactcc gctccgggtc gtcggttgcc caggcggact tcatctccag gacgccgaac
     3181 ggcacgcggt cgttgatcat attgcggaca gcgccttccc cgggctgccc cgcccattca
     3241 acgagcgcgt gcgagtcctc ggctgacccc agcggtccca tggctcgcac ccccgccacc
     3301 atctttcctg cgcggtcgat caccgcgaag aacatctcgg tgtcggcgcc cgtgcgcagc
     3361 gcgtcgacgt cgagggcgca ctcaacaccg tgcttgcggt agctgcgctg cgctccggcc
     3421 gcgtactcgc cccacaagtc cgggtcgacc gacggttgcg aaaccagcag cgtgcagtcg
     3481 atgtctggat cgtgccagct aaggccctca tcaaggcgga acctgtccac actcatggca
     3541 cccagcgaaa caacggtcat gcgacgtcct tagtgtcgtg gcgcgccgac ttccagtttg
     3601 tggaacccgc gctttgaata cgaataggat ggatttacgc ctcgtacaaa ccgcgtcccc
     3661 gccggatacg tattggcgtt ctttcgtcct ttctgttgcc gagttcgggc tcaaggaatg
     3721 taaacggcta gtggacgcgt tgcatcatat tacaaatccc aagtcgctca ccacctctaa
     3781 agcggcgctt tgcccaagcg gaaaagagcg gttcgtcaat gcggcaaact cgggttactt
     3841 tgcgcttttc agttcacgtc ggcgggcatc cgcttcggcg ccccgcgcgg tcgggcccgg
     3901 caaatgtccg tcagccatcc gcgttgccga ggtgccggag cggaggctcg caacaacttt
     3961 tctgatcggc gagcggccct gtgaactgca tgttcgtagc aattcagggt gccgggggag
     4021 gatcgttcac ctcgaattgg tggtagttcg aatccccggg tactactgca tgggcaggcc
     4081 ggaactaacg ccccgataat gcacgtcgtg acctttgcag gatttaatcc ccggagttgg
     4141 atcgcgacca tttgcgaaat tatttcgagc cttctcataa acaacaccca gcactcgatc
     4201 gtgcgcagcc cctgtcgatt actgtggtct aagagcgact tgactgcggc ttttctggtg
     4261 cggcgcaacg ggatcggtcc cgttcgactt gccccccatt acgcggtgta tcggactaac
     4321 tccgttcacc gggcctgccg gcacgtaatt cggcacgagt ctaatgtcag cccaaaaatt
     4381 agctgagtgt tgtctgagac aggcgtggcg ggaccggatt ccgtgtcgca cagccctagt
     4441 gtcggggcat gagcacgaag tactacctgc aaaaggtgcc cgtcgaatcc gtccagccag
     4501 gcttttccct ggcgatccac cgagatggcg actaccggct atttcaggtc gaatgcacgc
     4561 agatgtccca tcgctccggc cagtccgtgc agttcaagct catatcggag ccggtcagcg
     4621 gcggtgaccc ctgggtcctg gagtgcgaag ccggtacccc ggtggtccgc atcctgggcg
     4681 tctgcaaggc cgcctgctga acgcgcgatg cgcagcgggc cgggggtgga tcgacgccgc
     4741 ttcgcggagt taacgctgcc ccggcccgcc gtcgtccggc gcggccaggc aaaccggaga
     4801 actcgtcgat tcgtcctcgc cgttgcggtt gtcgactcgc cggcgatccg gtaggcctga
     4861 cgacggggcc gggtatcctt gccgcggccg gaaccgcccc ataacctggg caggcgcagt
     4921 agacgacggc atcacagacg ggctggtcat ggcgaaacgg tcatcgcgag tcgagggcct
     4981 cagcgacccg ccgggccgca attcggggat cagcgggata tccggggtat cacggtggcc
     5041 gaactgaccg gcgcgcgagc agaagagctc gcgacaatgg acatcttcga gggctgcagc
     5101 acccaagacc tagagccgtt ggcgggctgc ttgcagccgc tgcgcgccgc cgccgatcag
     5161 gtgctgatgc agcagggcga gcaggccgtc tcattcctgc tcatctcgtc gggtaccgcc
     5221 gaaatcaagc acgtcggaag cgacggtgag gtcgccgtcg gacagacgcc tcccggcacg
     5281 atcgtgggag agatcgccct gctgcgggac aaaccgagga tcgcgacggt caccacggcc
     5341 gaggcgctga ccgggtggat cggcaacaac gacgccttcg cccttatggt gcaaatcccc
     5401 ggggtgatgc cgcgcctttt gcgcaccgtg cgccagcgtc tcgccggatt catcacgccc
     5461 atcccgatcc aggctcgtga tggcaccgag ttgatgctgc gcccggtcct ccccggcgac
     5521 gacgagcgga ccatccaggg gcacatccaa ttctccagcg agacgctgta tcggcggttc
     5581 atgaccgccc gcgtgcccag cccggcgttg ctgcactatt tgtccgaggt cgactatgtc
     5641 gaccacttcg tctgggtagt gaccgacagc agcgacccgg tggccgacgc gcgctttgtt
     5701 cgcgacgaga ccgatccgaa gagcgccgag atcgcgttca cggtcgctga cgcgtatcag
     5761 ggccgcggga tcggtagctt tctgatcagc gcgctatcca ttgccgccaa agtcgacggg
     5821 gtcgagagat tctccgcgcg catgctttcc gacaacgcgg cgatgcgcgc gatcatggac
     5881 cattacgacg cggtctggca gcgcgaggac gtgggtgtca tcaccaccgt catcgatgtg
     5941 cccgaccgcc tggccttcgg acccgagatg gcgggcaaga tcaggcgcgt cgcccgccag
     6001 gtgatcgagg cggtcggctg acgtcgcgcg ggctgtcagc cagaacaccg gttcgcgcgg
     6061 ccgcatctcg tgatatcaac gggcatgcgg cccttccgga ttggcgctgt ggcgactccc
     6121 gtagtcacat tgctggtggc ggggtgcgct catgctccgg ccccgatgcc gtcggccaaa
     6181 cccactacgt cgcaggcggc cggcggcaac gccgtcaacc ccgccaacat caagcggctc
     6241 gtccgcgagc tgccgcccaa ttatgaggtg accgccggca tccccagcgg ggcctcacca
     6301 cgggtgatct ggagcctgga acccgatgca tcagatatca gggtcaaccc gccgcaatgc
     6361 gccacgctcg ccgacccggg cgacggacgc gaccaatccg cgcagggcgt gtccggatcg
     6421 ggtagcggcg gcatcgtcga cgcggtggtg gtggctctgc cgggcccggc ggagctcgac
     6481 cacgacttgg tggcggcgtg tggccggtgg accatgatcg acggccacac cacggtcagc
     6541 attcacctca ccgacccccc gcacctcgac ggcgtcgaaa ccctgggcat ggtggccgac
     6601 accaagtcat ccgtcgagtc gggcaccgaa atcgactcgc gggcatacac cttcaccgca
     6661 tacctgggga actattacgc gttcaccacg ctcaccacag atccgggttc ggcgctcccc
     6721 acgctgtcac cgcaatttgc cgccgatctg ctggtcaaaa cggtgtcgac gttgcgcggc
     6781 tgagcatctg tcttcggtag attggccacg atgttcaagg tgttgctcgc cgccgcatcc
     6841 gtctgcgcgc tcgccggctg ctcatcggga agcgatgcct cctcggccaa ggccgacatc
     6901 agcaaggtcg ccgacgtgaa gtcaagcttg ggaccggagt acagagtcag cgacatctcc
     6961 gaaagggcga tcgatcccaa gctgctgtcc gcacggaagc tgcccgacgg gctgaccttc
     7021 gatcccgcga actgcgcgaa agaggcggtg gggccggaca tgccaccgga tctgcagggg
     7081 aatatggccg ccgtctccgc tgagggcaac gggaaccggt tcgtcgtgat cgccgtggag
     7141 acctcaaaac ccgtgccgtt caacgacccc gggaaggact gcgccaaggt ggctttcgcg
     7201 ggggcccagg tgcggggcgg catcgaagtg atcgatgcgc cgcatatcga cgggactcag
     7261 acgatgggcg tgcatcgggt tctgcaggcg gtgctggacg ggggcacccg cacgggcgaa
     7321 ctctacgact attcggccca attcggggat taccaggtga tcgtcaccgc caatccgctt
     7381 gtcgtcccgg atcagccggt cgccaaggtc gacacgcaac gtgcccgcga cctgctcgtc
     7441 aaggcggtgg ccgccatccg ggcctagcgc acgtcgcggg gccggaactg gatgctgacg
     7501 cgcgggccgg tgggtgccga cgtcttgggg accgcgtgct cccaggtgcg ttgacaggat
     7561 ccgcccatca ccaggagatc gccgtgcgcc tgtggcagcc gcaaggacgc gccgccgccg
     7621 cgccgccgca gcgcgaaggt gcgggtggcg cccaggctga cgatggccac catggtgtcc
     7681 tgcgagctgc tgcggccgat ggtgtcgccg tgccaggcga cgctgtcgga gccgtcgcgg
     7741 tagcaacaca atcccacggt ggtgaaaggc tcgccgagct cgcccgcgta gatgtcgttg
     7801 agccgccggc gtagccgccc cagtagtgga tggggtggct cttcgaccgt caggtcgtga
     7861 aagctgacca gccgcggtac gtcgaccacc cgctcgtaca tctggcggcg ttcggcccgc
     7921 cacggcaccg tggacagcag ggcgtcgagc aggccttccg agcccgaggg ctcgtccgtc
     7981 agccagccgg cacgaacctc gatgaaggcg ccgtcgccta gttgtctgcg ttcggtgtgc
     8041 tcgaacagcg agccctgtac cgcgatcgcc acgcccgcca gtctatcgca cattcgttcg
     8101 atcaaacgag atgacggggc ttgttggagg gcacgcgagt cttatccgtt gttgttgcgc
     8161 ccgtgttcct ttggtgccgg tccggccgcg tcggcggggt cgagcggcgc tcgcaattgg
     8221 cccgcgcgca gcagtttcgt caccgcggca tcccagcgca tcagttggcc gcgggtagcg
     8281 aagggttcgg gcagatagcg ctcgatgtgg ggccgcagaa tgatggcgcc gacgcgcagg
     8341 atcagcggat tgagcacgcc ccagacgagg tcgaggtcgg gccgccccag cccttgttta
     8401 atgagctgat cgccctgagc ggcactgatc ccgaccaggc cgtcgaagat tatcgacccg
     8461 atcgcgtcgc gctcgaccaa ggcgcgtccc agatagtcga cgacgtcggc gtgctcggcg
     8521 atcagcgaca ccatccggag gcccgcttcg acgagagggt ccgctggcgg tccgggtagc
     8581 ggcgatggct cgatggcgcc acgcaccacc cccaacacgt atccgtcgat ggcctctatc
     8641 agctgtccct tggtgccgaa gtgatgctga atcagcccga tcgaaacgcc ggcggcgtcg
     8701 gcgatcatgc gcagggatgt cgcgtcaacg ccatggtggg ccaccacacg catcgcggcg
     8761 gcgcgaatcc gctcgggact ggaccgttcg tccgcaggac gccgacgggg aacacgcggc
     8821 gcactcacag cgtcaaccgt agtgactgct atcgcgtcgg ttgcgcagga gcgccggtgc
     8881 gcaggctatc agtggcggct cgtgttgctg tcgtgttgtt gtcgtgacgc tgtgtcgact
     8941 cagcggctct tgtcccagat gtgctcggtg acgtcttgga caccgaccag cgtgacgggg
     9001 tcgtcggcgc gcagcagcat cggccggctc actacgacct gcaagatcga accatcggcg
     9061 tggcgcaggg ccatcagctc accggctcgc tgctgcaaca gtgttgtcac cggcgtgtcg
     9121 agggcgggga cgtcccggat caactcgttc gcgaaaacgg cgctgaggtg actgcgatca
     9181 tggccgatca tctccgcaaa ggcgtcattc gtgtagacga cggtgtcgcc gtgcacagcc
     9241 cagataggca ctggtaagcg gtcgagcagg aggcgcgcgg gcatgcgatc gatgtagccg
     9301 agcggttccg ccggtggccg ttgccttcga tcttccaaaa cccctaccct cggtatggcc
     9361 aacgagaatt agcgtgcggt aaacccacgc caacgtgagc taatcgtgca tcagccgacc
     9421 caatggggca atacacctat attgcggctc gtgcggcgcc gcgctcaacg gccggcccta
     9481 tctgcgcggc gcggatgacg cgatccgcga gatcggcgcg actacggttt agctgtgggt
     9541 gtcgtcgagc tgggtaccaa ttccgaggtg ggcgcgctgc gcgtcgtcat cttgcaccgc
     9601 cccggcgccg agctgcggag actcaatcca cgcaacaatg accagctgct gttcgacggg
     9661 ttgccctggg ttgcgcgcgc ccaggaggag catgaccaat tcgccgggtt gctgcgttcc
     9721 cgcggtgtgg aggtcctgct gctatccgac ctgctgaccg aggcgctgca ccacagcggg
     9781 gccgcccgga ttcagggggt tgccgccgcg gtcgacgcgc gtcggctcgg agcgccgttg
     9841 gcgcaagagc tttcggcgta tctgcggacc ctggagccgg tcaggctggc cgaggtgctg
     9901 atggcgggaa tgacgttcaa cgagctgccg tcggccaccc ggagcgacgt gtcgttggtg
     9961 cttcgcatgc accacggggc ggatttcgtc atcgagccac tgccgaacct ggtgttcact
    10021 cgtgactcgt cgatctggat cgggccgcgg gtggtgatcc cgacgctggc gctgcgggcg
    10081 cgggttcgcg aagcgtcgtt gaccgatctc atctatgccc accacccacg gttcaccggg
    10141 gtgcggcgcg cctacgagtc gcgcaccgcc cccgtcgagg gcggcgacgt actgctgttg
    10201 tcctccggcg tggtcgcggt cggggtcggg gagcgaacca ccccggccgg tgcggaagcg
    10261 ttggcgcgca gcctcttcga cgacgacctg gcgaacacgg tgctcgccgt cccgatcgcc
    10321 cagcggcgcg cgcaaatgca cctcgacacg gtgtgcacaa tggtcgacac cgacaccgtg
    10381 gtcatgtacg ccaacgtcgt cgacacgctc tcggcgttca cgattgagca cacccccgat
    10441 ggcgtcacca tcagcgatga aacgccattt ctggaagccg cggcgaaggc catggggatc
    10501 gacaagctac acgtcatcga caccggtttg gaccccgtga tcgcagagcg cgagcagtgg
    10561 gacgacggca acaacacgtt ggctctggcg ccgggcattg tagtcgccta cgagcgcaac
    10621 gtccagacca acacccgcct gcaggacgcg ggtatcgaag tgctgaccat tgcgggctcc
    10681 gaactgagca ccggtcgcgg cggtccccgg tgcatgtcat gtccggtcgc ccgtgacctg
    10741 atatagccgt ttggccccag attgccgtgg cggtcgtggt tggcgcgcgt atcgcagccg
    10801 tcacggcaat ctcgggggag gtagaagcac cagcgcatcg cgtgcccgtt gcacgatgac
    10861 gttcggccgg tcctcgttga tcaccctgat cacgtgccaa ccaaggcggt cgaacatctc
    10921 ggctcgacgg atatcgctca cgtattgcct tcgatcggtg cggtgttggt cgccgtcata
    10981 ctccagtgcc accatctgca tttcccatcc catatccagg tacgccacga ggtgcccatc
    11041 gctgactcgg atctgcgtcc ttggccttgg taagccggca tcgatcagtg cgagccgcag
    11101 ccagctttct ttcggcgatt gagcgccact gtccatgagc gacagggcgt ttcggcactg
    11161 acggacgccc cttgcgcccc tgtggcgttc aatcaacggc gtcacgtccg ccgcggtgac
    11221 gccagtcgcg gctgacagcg catcaaggtg ggtgaccgcg acggcgcggg gcagatggcg
    11281 ggccaaatcg aacgcggtgc gtgccggatt cgtcccaagt aatccgcgca tttcgacgag
    11341 atccgcaacg gcgatccgtt cgcggcgaac gatgatgcca ggggcgggat ggttgaacgg
    11401 cccgacgacc tctaccgggg tgaattcgtc gacccatttg gctccgtgca gcgcggcggc
    11461 cgcgcggccc gcgatgattc cccgacgacc tgaccacagc caggctgcgt agatattgct
    11521 ccacaacgac ctgggcgcgt tcttcggcag gtagacattc cgatggatcc ggcggtaatt
    11581 ccaacgcaat tgggcgcgcg tcagagtgcc tcgtgcgact gcttcgctgc ccagaaacac
    11641 cctcgtcacg cggtcatcat gcgcgccatc actgacacgt cggcgagatt gccgtgatgg
    11701 tcgtggttgg tgcacgtatc gcagccgtcg tggcaatctc ggggaggtac tgcggggctc
    11761 tacggcgaga ttgccgtagc ggtcgtggtt ggcgccggaa tcgcagccgt cacggcaatc
    11821 tcgggggagg tagaagctag cgccagctgg gcagccagat ctccatgttc caggtctgct
    11881 gcgagatggg caggccggtg agcaccggaa acagccacgc gaagttggtc accaccaacg
    11941 cggtgtaaca cgacacggcg atgagcccca gcgtgcgtcg ttccgagttc tgaccgagtg
    12001 cccggccggc gggatagagg atgtcgccgc agatcagcgc gatcgccatg accaggaacg
    12061 gcgccatcgt cgcggcgtag aagaagtaca tctgccggtc gatgtcggcg aaccagggca
    12121 gccagcctgc gcagtagccc acccagacca ccgcatagcg ccagtcgcgg cggaccagca
    12181 tccgccaggt cgcgtagagc agcaccggcg ccgccaccca ccacatggcg ggagtgccca
    12241 ccagcatctc cgccttgacg cacgactgcg cgccgcaacc gggaacgttc tgctggtcga
    12301 tggcatacaa caccggccgc aatgacatgg gccagctcca cggtttggat tcccacgggt
    12361 ggtagttgcc ggcggaattc gtcaggcccg catggaattg caacgccttt gcggtgtagt
    12421 gccacagcga gcgaatcgcg tccgggagcg ggacaaccga gtggggacca atggacaggc
    12481 cgacctcatg gcggtcgatc gcggtctccg aggcgaacca gggcgcgtag ctggccagat
    12541 ataccgcgac cgggatcagc cccagcgcgt acgtggtggg cagcaggtcg cggcgcaccg
    12601 tcccgagcca gggtctggtc acctggtact cgcgccgcgc ggccacgtcg aacgccaacg
    12661 acatggcacc gaagaacacc acgaagtaaa gaccggacca ctttgtcccg catgccaatc
    12721 cgagcagcac cccggcgccg aaccgccacc agcgcacgcc cagccgcgga ccccaaacgg
    12781 tttcggcgct gcgaccctcc aacagcgcca agtacatccg ctcgcgtatc tgatcgcggt
    12841 cgacaatgag cgcgccgaac gccgcgaccg cgaagaaggt gaggaagccg tcgagcagcg
    12901 cgctgcgcgc ggtgacgaag ctgaccccgt cgcagatgag cagcacgccg gcgatggcgc
    12961 cgaccagggt cgagcggctg atccgccgca cgatccgcat caccagcgtc accatgacca
    13021 cgccgagcag cgcgccagtg aaccgccagc ccacgccgtt gtagccgaag atcgcctccc
    13081 cgatcgcgat cagttgcttg ccgaccgggg gatgaacgac cagaccgaac ccgggattgt
    13141 cttccacccc atggttgtgc agcacctgcc aggcctgggg cgcgtaatgc ttctcgtcga
    13201 agacgggtgt gccggcgtcg gtcggcgagc ccacattgag gaaccgggtc accgttgcca
    13261 aaactgtgat cacgccggtg gtgatccagc cgcggatgtg gtctgtcggc ccaaagtccg
    13321 cgaccggaac cagcggcccc gggctcacga cgggtacgac gcgctcgacg cgatcttgaa
    13381 cggcgacggg gctttcgccg ggcggggcgg tcatcacgtc gatcgtaggc tgtccgtcat
    13441 gaccgctggt cttctgttgt tgggcgcgac ccccctgggg cagccgtcgg atgcctcgcg
    13501 acgcctgatc gacgcgctga cccacgccga cgtggtggcc gccgaagaca cccggcgggt
    13561 gcggatcctg gcaaaggcgc tcgacgcccc gatcactggc cgggtggtca gcctgttcga
    13621 ccgggtcgag tccgcgcggg tgcccgccct ggtcgacgcg atcaaagccg gcgcgacggt
    13681 attgctggtc agcgacgccg gaatgccgtt gatcagcgac cctggctacc ggctggtcgc
    13741 ggcgtgcgtg gatgccgggt tgccggtgca gtgcctgccc gggccgtccg cggtcacgac
    13801 cgcgctggcc gtgtccggcc tgccctcaga aaggttctgc ttcgagggct tcgctccgcg
    13861 caagaagtcg gcgcgccagg cgtggttggc ctcgctggcc gacgagcggc gcacctgcgt
    13921 gttctttgaa tcaccgcgtc ggttggacgc gtgcctgcat gacgccgtcg agcagcttgg
    13981 cggggcgcgt cgggccgcgg tgtgccggga gctgaccaag gtgcacgagg aaatcgtgcg
    14041 cgggtcgctg gacgagctgg cgaggtgggc ggccgacggg gtgctcggcg agatcaccgt
    14101 tgtgctcgcc ggcgcgactc cgcgcgcgga tgtcccggca ctgttggcag aagtggagga
    14161 gctggtcgcc gcgggggctc gcgtcaagga cgcgtgcagc caggtggccg cggggcatcc
    14221 ggaagtgcgc tcaagccagc tctacgacgc gattctgcaa ttgcgcgacg agtgagctga
    14281 tccgaccggt gccggtcagg tcagggcgtg cagaccccga tgggaccgaa tccggggctg
    14341 cacaacctcg ggtgcggaat ggccggcggg gccggcagag ccggcagtgg gggaggcgcc
    14401 ggcagggccg gtggggccag tggaagggcc ggcagcgcgg gtgccgcggg gagacccacc
    14461 gccggcagcg cgggtgccgc ggggagaccc accgctggga gcgcgggtgc cgccgggaga
    14521 cccaccgccg ggagacccac cgccgggata gccgcaccca ccgccgggag ggttgcggcg
    14581 atccctggga tagccgctgc cgcagcggga agaccaacgc cggggataga aatcccaggt
    14641 agcgacggca gactcacggc cggcacgagg cccgcctggt gcagataggt gtaggcaatg
    14701 gcacccattt ccaccgtgct aattgcggtc gctcccacgc ccaccaggct gcctcccacg
    14761 cccaccacgc cctgcacgac tgacaaggca acggccgcgc tgttggggat ggcggccagg
    14821 ccggccaggc cgccgaggcc cggtacctgg cccgccaggg cgtaggggtc aataccgccg
    14881 ggcaggccca ggtcgggcag cccgccgacg ccgcccacgt caggcaggcc ggccccggac
    14941 acgcctccgc ccggtacgcc gcccacgccg agcccgggtc cgcccacccc cggcaccgca
    15001 cgggcgcccg acggcacgct ggccgtgcct ggttctgtgg gtacaccagg ggcaattaca
    15061 ttggccgccg ttgaaaaggg cgtgggtggt ggggcgggcg cagccgccgg tgccgacggt
    15121 ctcggcgtct cgggcatcag gacggacgcg accttcgcgc atacggcgcc gccgctgccg
    15181 caggttgctg cggtaggcag cggtgtgctg accgaagtca gcggcatagc cgccagcgcc
    15241 ccacctatca cggcggacgc tgcggcgccg acaacggcga ttctcatgac ggacccctcc
    15301 agcaggcgac ccacgaccgg cgcgggtgat ggctgaagct tatgcgcacc cggctcgtga
    15361 tgcggaatta tcacaaattc gggacgagca cgctgttaga acagctaacc gaccgctaac
    15421 cgaccacagc tgcggccgcg atgcagggca gcccgacgat cggggcggcc ttgtgcaggc
    15481 attcctccca ctcggcatcg ggatcggagt ctgcggtgat cccgccgccg acgccgagca
    15541 cggcgccacc gtcggcgtgg aactcgacgg tacggattgc cacgttcagc tcgcaaccgg
    15601 cgatcggcga agccaaacca actgtgccgc aatatattcc gcggcgacga tgttcccact
    15661 gcgaaatcag ctgacgagcg cgatgtttgg gggttccggt gaccgaggcc ggcgggaagg
    15721 tggcgtccag cagcgccgac gtcggtagct ccacgggaac ccgcgccgac accgtggaca
    15781 ccaggtgcca cacgcccggc gcgcggcgca cgaccaacag ctcaggcacg gtgaccgtgc
    15841 ccacgatcgc cacccgccca aggtcattgc ggaccaagtc cacgatcatg atgttttcgg
    15901 ccacctcttt cgccgaagcc cgcagtgccg acggccaggc atccagcggc agggtgccct
    15961 tgatcgggct ggatgttacg accgtctcgc ggcgccgcaa gaaaagctcc ggagacagcg
    16021 atgccaccgc accccagggt ccggcaacga aggccgcgcg ggccggggag gtacgcgcga
    16081 cgccgtctac gaagaagtcc agcggggccc cgctgacggt cccggcgaat tgcgtgcaca
    16141 cgcaagcctg atatacctcg ccggcgcgga tcgcctccag acaggccagc accccgtcgc
    16201 ggtgcgccgc ccggtcggcg gcgtcccagt cgatccgaca atcgcgcggc ggtgcaggcc
    16261 ttgccgcgag agcggcggtc aaccagtccg gcatcggtgc gcccgacagg ctttcgtacc
    16321 accactgccc gtccgggtcg cggcgcagca cgcagtcggt ccatccgccc gcggcctcgg
    16381 gaatccggtt cggtcgcccg tcggcacccg gatccggata cgacagatag ccgacccagc
    16441 cgccgcccac cgctcgggcg taggacgcct gcgagtccgg cgggaccgcg aatgcgtcgt
    16501 cgatgctgac cggctgcacc gacacgctgg gcgcgatgac cgccagcgca ccgaaccagt
    16561 cgcccgtcag ggcggccggt ggtggcagat cgagcctgct ggtggcgtca ccgacggcac
    16621 gcagcacgcg gggtgcggcg ccgaggtcgc cgagccggtc gattcgcacc gctctagctt
    16681 gacatcagtg tcacggttat cgcgccgcag gcgctcggcg ctggagtgca tcgtcgccaa
    16741 gctgggtcgg atcgccaagc tagccacggc tgatgtcgcg tgcggccgcg agggtcgtga
    16801 gcttgtcggg attgcgcatc acatagaagt tggtgatctt gtcgtcgacg atctccaccg
    16861 tgatcgccat ttcgaggtgc tcgtcgtcgt agaccaacac cgccggagcg ctgttacagg
    16921 tcaccagttc cacgcgcagc gccgcccaca tctcctgcgt cttgcgcatc aaaccgacga
    16981 tcgccctggc tacccgtgcc gcgccgacca ccggcctgcg ggccgcgctc accttgccgc
    17041 cgctgtcggc ggtccaggtg gcgtcaggtg cgagcatcac catcagcgat tccacatcac
    17101 cgctgaccgc cgtggccaag aactgcgcgg tgacctcggc gttgcgttcc gggtctatcg
    17161 acatcttcgc ggcgccgccc cctttgcgcc gcgcccggac gtgttcgcgc gcgcggtggg
    17221 cgacctgtcg cacggtcggc gccggtttgc ccaccgcctc ggcgatctcg cggtagtcga
    17281 aaccgaacac ctcgcgcaac acgaacaccg cccgttcgtc agggctcagc gtttccagca
    17341 gtaccagcat cgcgatcgaa accgattccg ccagaacgac atcggctgac gggtcctggt
    17401 cgtcgagcaa cagcggctcg ggtagccagg gccccacgta atcctcgcgg cgccgcgcgc
    17461 cggcacgcag cgcgttgagc gcctgccggg tgaccagctg ggccaggtag gacttggtgt
    17521 cgcgcacgcc cgacaggtcg actgccgccc agcgcaggta gctgtcctgc aacacgtcgt
    17581 cggcctcggt cgccgagccg aggatttcgt aggcgatggt gaacagcaac ggccgcagca
    17641 gggtgaaccg ttctgcatgc tcaccaccgc gcgagggggt cattggagcg caacctgcgg
    17701 gccggccggc acggtcgcgg gccggttgcc gcctttgagc cagacgtacg agccgggttt
    17761 ggtcgcctcg cgccggatgg cccacagcgt gcccttgcag atcgcttcct tgatgggggc
    17821 cgcgacccgc cccgcgagaa ccacgttcac cggggtgtca tcggtgcggg tgagctggaa
    17881 ggtggcatgc gcgcggccca tgctgatgca ctgcccgacg aacgcctggt tgagcaccgc
    17941 gggcgcggtg ccggcgatgc ggctgagcac cgtgttggcg gcttgggcgc ccagtggccc
    18001 ggccgcttgg cagctcatcc gcaacggctg acccgacggc gctgcggcgt cgcccgcggc
    18061 gacgacgcga tcgtcgtcga tgctggtcat ggtttcgtcg gtgagcagcc ggccaacggc
    18121 gtcggtgcga aacccactgc gcgcggccag atccggcacg gcaaaacctg cggtccacac
    18181 cgtcgcggcg ctgggcagca ccgagccgtc atcaagcacg accgcgtccc aacgcacctg
    18241 gctgaccctg acgggttcca gcacgttggc gccgaggttg cgcagttgcc tcgccaccga
    18301 ccgccggccc cgtctgctca gcgatggacc gaggacgcca ccgcagacca gcgttacctg
    18361 ccggccccgc tccgccagtt cggaggccgt ttcaatgccg gtcagtccgc caccgacgac
    18421 ggtgatgggg gcggtcggcg gcaggtcggc cagtgcgtag cgcagccgct gcgcgccttc
    18481 cagatcggct atcgagtggc cgaattcggc gagtccgggc acctcaggca gccgggacag
    18541 cttggccgcc accgcgccca tacttccgac ggcgtagatc aggtagtcgt aggcgagctc
    18601 ggcgcccgat tccagcagga cgcggcggtc ggcggcgttg atgcgctcga cggtgtcgac
    18661 gaccagccgg attccgtcgc cgagcagcgt gtcgtacgcg gccgtcgcgg acccggtgtc
    18721 ggcgaccaac tggtgcaggc ggatccgctc gacgaatacc ggacgcgggt tgaccagggt
    18781 gacgtcgacg tcggcacgct gccgcagatg attggcggcc agggttccgg cgtatccgcc
    18841 gcctacaacg acgacgtgag tgcgtggggt ggtcatctcg gtcattcctg tctcctacta
    18901 tcgcggggct tgtgcccttg agacaccggg ggccgaccag gcgtgacaat aacgccgtca
    18961 atgtgagctg cctcacttac aggggacgcc gacgacgccg ttgacgcggg cgcggtgggc
    19021 cacccggttg ccctggctcc ggagtgattc gccgaagcgt tgcgggtttg ctggatgtgt
    19081 ggcgccagaa ccgaagcgga ggccgatgcg cgtggcgacg gggcaaattg ccctcgggcg
    19141 ggaagcgcca tgcgtgatac gctgcgctgc accgaatcgt gacctgcccc ggtcgggcgc
    19201 acccaccaga caagccgcga agtaggactc gaaagtgccc gtccgcgcgc gctgggagaa
    19261 gtaacctgta agacctacgc atccccctga ttgggccccg gtaaggccaa agcccagcag
    19321 ggagatgtgt gcccacatcg gaggagctcg tgccgcgcag cctggatctg atcgtcacct
    19381 ccgtcgcctc ccaactgatg gaggccaccg gagccacggc gacccaggtg agtgaaaggg
    19441 ttctcgcgca actcgtcgag cagttcgatg tggacgccag cttcttgcgc catcacgacc
    19501 acgagatccg ggcctcgata ctggtcgccg agtggccacc ccgcgacaac gttccagacc
    19561 cggatccact ggccgtcgcc catttcacca gcgcagatcc cgtgctcgca cagtgcgagc
    19621 atggcaggaa gccggtggtg gtccggccgg aaccaagcag tcgctacttc caacgtttgg
    19681 tcgggggaag caagcaggtc acctcgccct cggttgccgc cgcgccactg gtctcggggg
    19741 agatgaccac cggcgtgctc gggttcgtca aatacggcgc cagaaagtgg aagcaagagg
    19801 aactcaacac gctggaggcg gtcgcggctc tgttcgcgca gctgcaggcc cgcatcgcgg
    19861 ccgaagagaa gctccgctat ctggctgagc acgacgattt gaccggcctc tacaaccgcc
    19921 gggcgctggt ggcgcatctg tctgagaggc tctcggcggg aagccccgga cccgtcgcgg
    19981 tcttgtacct cgacctcgac cggctcaagc cgatcaacga ctacctcggc cacaccgcgg
    20041 gcgactggtt catcagggtc ttcgcgcaac gcatccgggg gtgcgcgggt cagagcatga
    20101 tcgcccggct gggtggcgac gagttcatcg tcattccgga tcaaacgatg tccagcgggg
    20161 ccgcggaatc gttcgcgcgt cggctggcgg gcatgctgtg cgagcgcttg gccatcggcg
    20221 ggcacatgat cacccgaacc gtcagcatcg gggtgacggt gggcgtgcca gggcgcgaca
    20281 acagcaccga cttgttgcgt cgtgccgatg aggcggtgct gacagccaag cgtgcgggcg
    20341 gtaatcaggt ggcgatgtcg accgatgaca tgtcgctgaa aagcgccttc cgcaatgaca
    20401 tcgagctgca tctgcaaggc gatatcgaca gcgaagcgtt gctgctgcac tatctgcccg
    20461 aggtcgacct gtggaccggg gccatcgtgg ccgcggaggc actggtgcgc tggcagcacc
    20521 cgatctgggg gctgctgctg ccggattcgt tcatcggggt ggccgaaacg accaacttgg
    20581 ccggggagct gggccggtgg gtgatgcgga ccgcgtgcgc cgaattcagc cgctggcgtt
    20641 ccaagggcat cggccaaaac gtcgcgttgc gcatcaacgt gtcgccggtt cagctgatca
    20701 cgcggggttt cgtgcgcagc gttgccgaca ccatcgaaga gttcggcatc gacgccggat
    20761 cggtgtgtct ggagatcacc gaacgtgcag tggtgcacga catcgaaacc acgcggaaga
    20821 ccctggcgga gctcaaggaa gtcggggttc agatcgccat cgatgacttc ggcaccgggt
    20881 acgcggtttt gtcgcacctg aagtccctgc ccgtcgacat gctcaagatc gacaacggat
    20941 tcgtccgtga cctgggcacc aacgccggcg atctggccat cgtccgggcg atcatcggcc
    21001 tggctgaggc cttcggcctg caactggtcg ccgagggagt cgagacaccc gctgccgcac
    21061 tgactttgat gcaacacggc tgccaccggg cgcagggatt cttattgtcc agaccggtcc
    21121 cgggcgacgc catggaagct ttgctggccg cgcgctggat gcc
//
LOCUS       DASSGQ010000311        11602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_752026,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000311 DASSGQ010000000
VERSION     DASSGQ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11602
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752026"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..701
                     /locus_tag="VFI55_13715"
     CDS             177..701
                     /locus_tag="VFI55_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HET7075124.1"
                     /translation="MSRLSNALRGTAATMLTGGLALAGAPIVNADPANTGNPADTNTL
                     ATSLSKGYGLNNCTAQNISSGELASLTCGQSPDPAGPVQAKYILFSSADNLAGSFKAS
                     IKDDVLAACGDSGQSPTTWHQGSSSNSGSVACGTYQNAAEVIWTTDTKNILSYIRAAN
                     TDIPSLYQWWRANG"
     gene            765..1292
                     /locus_tag="VFI55_13720"
     CDS             765..1292
                     /locus_tag="VFI55_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HET7075125.1"
                     /translation="MSHLSKALRAAAATVLTGGFALAGAATATADPSNTGSSADTNTL
                     AASLSKGYGLNNCQSGQLSGPVLAELDCGQNPDSTGPAAGVYQLFPNATDLAASFTAS
                     IKDVSLSACGDTGQSPGTWHQGSSGQTAGQVACGTYQGAATITWTTDSKNVLSHIRAS
                     NTDVNALYQWWRTNG"
     gene            complement(1307..2074)
                     /locus_tag="VFI55_13725"
     CDS             complement(1307..2074)
                     /locus_tag="VFI55_13725"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET7075126.1"
                     /translation="MKRYVALGSSMAAGPGIRPRAAGAPRWSGRSAHNYAHLVADRRN
                     LELVDVTFSGATTAHVLAERQHGEPAQLAALDGSESLVTITIGGNDTGYVPLLIAASL
                     PRAARRLPLLGGRISELLDRDGRDRALAAVFDSLCAIGVAVRERAAGARIFFVDYLTI
                     LPPAGAPAPPLAEADADLGRHVAATLERLTADAAAATGCDVVSAAAASRPHHAWSAEP
                     WTTRPARYVVPMPGRPAPLHPNGAGMHAVAELVLNQL"
     gene            complement(2121..2942)
                     /locus_tag="VFI55_13730"
     CDS             complement(2121..2942)
                     /locus_tag="VFI55_13730"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014712465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HET7075127.1"
                     /translation="MADSKLTIADRSFASRLIMGTGGATSLAVLEEALIASGTELTTV
                     AMRRVDAEGGTGLLDLLNRLGITPLPNTAGCRSAAEAVLTAQLAREALSTNWVKLEVI
                     ADERTLLPDAVELVRAAEQLVDDGFVVLPYTNDDPVLALRLEDIGCAAVMPLGSPIGT
                     GLGIINPHNIEMIVARAGVPVVLDAGIGTASDAALAMELGCDAVLLASAVTRAADPPA
                     MAAAMAAAITAGHLARRAGRIPKRFWAQASSPATMRAAQRPVVGAPPACGGEPGN"
     gene            complement(2935..3144)
                     /gene="thiS"
                     /locus_tag="VFI55_13735"
     CDS             complement(2935..3144)
                     /gene="thiS"
                     /locus_tag="VFI55_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HET7075128.1"
                     /translation="MIVMVNDKQIEVDEQTTVAALLQSLGFPDRGIAVAIDFSVLPRS
                     DWTTKLLDFSGRPVRLEVVTAVQGG"
     gene            complement(3141..4163)
                     /gene="thiO"
                     /locus_tag="VFI55_13740"
     CDS             complement(3141..4163)
                     /gene="thiO"
                     /locus_tag="VFI55_13740"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HET7075129.1"
                     /translation="MPAPTPTGLLAVIGGGVIGLSVARRAAQAGWSVRVHRSGEPGAS
                     WVAAGMLAPHSEGWPGEERLLRLGLESLRLWREGGFLDGLPPEVVTARESLVVAVDRA
                     DVADLRTMADWLSEQGHPVVWESAARDVEPLLAQGIRHGFRAPTELAVDNRALLEALS
                     LACERLGVRWAAPVQDLSCVDGDAVVIANGIDAPALWPGLPVRPVKGEVLRLRWRKGC
                     MPLLQRVIRARVHGRQVYLVPRADGVVVGATQYEHGRDTAPVVSGVRDLLDDACVVLP
                     ALGEYELAECAAGLRPMTPDNLPLVQRLDARTLVAAGHGRSGFLLAPWTAEQIVSELV
                     PIGARA"
     regulatory      complement(4164..4299)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4343..5032
                     /gene="thiE"
                     /locus_tag="VFI55_13745"
     CDS             4343..5032
                     /gene="thiE"
                     /locus_tag="VFI55_13745"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET7075130.1"
                     /translation="MSLGRPAARLAAARLYLCTDARRERGDLAQFVDAALAGGVDIVQ
                     LRDKGSAGEQRFGPLEARDELAACEILADAARRHGALFAVNDRADIARAAGADVLHLG
                     QGDLPPGVAREIVGPDMVIGLSSHDQTQATAAATGMADYFCVGPCWPTPTKPGRRAPG
                     LELVRATAQLVSALGGDRPWFAIGGIDARRLPEVLDAGARRIVVVRAITAAEDPRAAA
                     EQLRSALAAAS"
     gene            complement(5004..5645)
                     /locus_tag="VFI55_13750"
     CDS             complement(5004..5645)
                     /locus_tag="VFI55_13750"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7075131.1"
                     /translation="MQGDGDGWVISDSGAHYWGRYGAAGLLLRAPRPDGTPAVLLQHR
                     AWWSHQGGTWALPGGARDSHETPEQTAVREAGEEAGLPVERFAVRGAVVTAEVAGIGG
                     AHWTYTTVVADAAELLHTVPNRESTELRWVGEDEVADLPLHPGFAAGWDRLRTAPALV
                     PLGDGDERRQRLPRTVEVETGVFVWCMPGPPLPADQAPSPLSLRINSLLQAPT"
     gene            5796..7115
                     /gene="glnX"
                     /locus_tag="VFI55_13755"
     CDS             5796..7115
                     /gene="glnX"
                     /locus_tag="VFI55_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase G-activating protein GlnX"
                     /protein_id="HET7075132.1"
                     /translation="MTVELAHPSTEPKGSRSPAEPAHPRWWFISTTPGRILTIGIVLA
                     ALGVSSAFATSTTINHRQQVLTTVLNHTEPLSFAAGRLYTTLSVADAAAATAFIAQAE
                     PQTVRQRYEQAITDAAEAVTRASSGLTDEPAVQLLGRINAELAVYTGLIEIARTNNRE
                     GNPVGSSYLSEASGLMQSTILPDAAQLYQATSERVDTETTASTHFPAPVILVVAGTLV
                     FGAFSHRWLARRTRRRVNPGLVVGALGILVMVVWVGTALTISTTASRSAKDTAAESLK
                     TVTNVAITAQQARADETLSLIRRGDEEIRKQSFYQRIDSMHQQLDEYMARSDAVDKPD
                     LQGADQLLVRWRQANDRINSYISVGNYRAATQVALGSGEDDSTPAFDKLEDQLGKAMD
                     QSRTHLRNDVLNARSGLSGAQVGGVVLSLGAAIAVALGLWPRLKEYR"
     gene            7115..8110
                     /locus_tag="VFI55_13760"
     CDS             7115..8110
                     /locus_tag="VFI55_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7075133.1"
                     /translation="MAGLPRLPRFRPASYRLGVFAAAVVLAGCSRTESLTVATVPTLP
                     PPTPIGMELLPPQPPLPADDASQDCNATASLRPFPTKADADAAVADIRARGRLIVGLD
                     IGSNLFSFRDPITGEITGFDVDIAGEIARDIFGAPSHVEYRILSSDERVTALQKSEVD
                     VVVKTMTITCERRKLVNFSTVYLDASQRILVPRDSPISRVADLSGKRVCVARGTTSLH
                     RIQQIDPPPDIVSVVSWADCLVAMQQREIDAVSTDDSILAGLVEEDPYLHIVGPNMAT
                     QPYGVGVSLNNTGLVRFVNGTLERIRRDGTWNTLYRKWLTVLGPAPAPPTPRYVD"
     gene            8122..10422
                     /locus_tag="VFI55_13765"
     CDS             8122..10422
                     /locus_tag="VFI55_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase PknG"
                     /protein_id="HET7075134.1"
                     /translation="MDAKGAADAKDPEDLAPGTQPADVQSGPLSEGRPQATQALFRPD
                     FDDDDDDLPLFTGAAIDTEPQDRMTVATRVLPPIRQLGGGLVEIPRMRDIDPLEALMT
                     NPVVPESKRFCWNCGKPVGRSGSQDSEDGKGSSEGWCPACGSPYSFLPQLNPGDIVAG
                     QYEVKGCIAHGGLGWVYLAVDHNVNDRPVVLKGLVHSGDAEAQAIAMAERQFLAEVVH
                     PQIVQIFNFVEHTDRHGDPVGYIVMEYIGGQSLKRSLKNGKAEKLPVAEAIAYLLEIL
                     PALSYLHSIGLVYNDLKPENIMLTEEQLKLIDLGAVSRINSFGYLYGTPGFQAPEIVR
                     TGPTIATDIYTVGRTLAALTLNLRTRNGRYVDGLPEDDPVLGTYDSFARLLRRAIDPD
                     PRRRFCSAEEMSAQLMGVLREVVAQDTDVPRPGLSTIFSPSRSTFGVDLLVAHTDVYL
                     DGQVHSERLTAREIVTALPVPLVDPTDVAAPVLQATVLSQPVQTLDSLRAARHGSLDA
                     DGIELSESIELPLMEVRALLDLGDVAKATRKLDDLAERAGWRWRLVWYRAVAELLTGD
                     YDSATKHFTEVLDTFPGELGPKLALAATIELARNTDEHWFYQTVWKTNDGVISAAFGL
                     ARSLSAEGDRTGAVRTLDEVPPTSRHFTTARLTSAVTLMSGRSTGEITEAQIRDAARR
                     VEALPPTEPRVLQIRALVLGGAMDWLEDGHEASTNHILGFPFTEHGLRLGVEASLRSL
                     ARVAPTQRHRYTLVDMANRVRPTSTF"
     gene            complement(10537..11397)
                     /locus_tag="VFI55_13770"
     CDS             complement(10537..11397)
                     /locus_tag="VFI55_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HET7075135.1"
                     /translation="MRDAREPFIGSEALASGTINRHQLRTRYRAVLPNVYLSNDVQLS
                     LELRIFAAWLWSKRTAIIVGAAAAALHGARWIPEDVPVELLYNNSRPPQGVLTRRDVL
                     LDGETQTIAGRPVSTPERTAFDIGRRGAVRSAVVRLDALARATGFKVDDVLRIAKCHP
                     RSPGLRRLESALVLVDAGAQSPRESYLRLLLIDAGLPRPQTQIPVQGVDGMPVAYLDL
                     GWAEYMVAVEYDGDQHRTDRRQFVKDIRRIDMLEQMGWIVIRVVAEDRPANILRRVRE
                     ALAGRRSTVY"
BASE COUNT         1944 a         4123 c         3823 g         1712 t
ORIGIN      
        1 ggataccgcg ggaaggatgc ccagacagaa aatttccggc ggaatgtgtt gcatagtcag
       61 gacgtagccc tacgatccca tttagcaaaa gcactgtttc cggcgaggaa cgcaaaatac
      121 actcctggat gtcagcgcac ctgcaccgcc ggtcggacaa ccgctaggag cctaggatgt
      181 ctcgtctcag caacgcgttg cgcggtacgg cggcgacgat gctgacggga ggtttggctc
      241 tcgcgggtgc gcccatcgtg aacgcggacc ccgccaacac cggaaacccg gcggatacca
      301 acacgctggc cacctcgttg tcgaagggct acggcctcaa caactgcacc gcccagaaca
      361 tcagcagcgg cgaactggcc tcactcactt gcgggcagag ccccgacccc gccgggcccg
      421 tccaggcgaa gtacatcctg ttcagcagtg ccgacaattt ggcgggctcg ttcaaagcca
      481 gcatcaaaga cgacgtcctg gccgcatgcg gggatagtgg ccagtcgcct accacctggc
      541 accagggcag ttccagcaac tcgggatccg tggcctgcgg gacctaccag aatgccgccg
      601 aggtcatctg gaccacggac accaagaaca tactcagcta catccgcgca gccaacaccg
      661 acatcccgtc gctctaccag tggtggcggg caaacggctg acgttcgcgg ttgggccgca
      721 atcatttcca acacgacagt ttgaaaacac gtaaaggagt caggatgtct catctcagca
      781 aggcgctgcg agccgcggcg gccacggtgc tcacgggcgg tttcgccctc gcgggtgccg
      841 ccacggcgac ggcggacccc agcaacaccg gcagttcggc ggataccaac acgctggcag
      901 cctcgttgtc gaagggctac ggcctcaaca actgtcaatc gggtcagttg tccggtcctg
      961 tgcttgctga actcgattgc gggcaaaacc ccgattcgac tggaccggcg gccggcgtct
     1021 accaactctt ccccaacgcc accgacttgg ccgcctcgtt cacggccagc atcaaagacg
     1081 tgtccctgag tgcatgcggg gacaccggtc aatcgccggg cacctggcac cagggcagtt
     1141 ccggccagac agcgggacag gtggcgtgcg gcacctacca gggcgccgcg acgataacgt
     1201 ggaccaccga tagcaaaaac gtgctgagcc acatccgcgc atccaacacc gacgtcaacg
     1261 cgctgtacca gtggtggcgc accaacggct gagccccgaa gttggactac agctgattta
     1321 gcaccaactc cgccaccgcg tgcatcccgg caccgttggg gtgcagcggt gccggccggc
     1381 ccggcatcgg cacaacgtaa cgcgccggcc tggtcgtcca cggctccgcc gaccaggcgt
     1441 ggtgtggccg gctggccgcc gcggcgctga cgacgtcgca gccggttgcc gcggcggcgt
     1501 cggccgtgag tcgttcgagc gtcgcggcca catgccgacc cagatcggca tccgcttctg
     1561 cgaggggcgg agccggcgca cccgccggcg gcaggatcgt caggtagtcg acgaagaaaa
     1621 tccgggcgcc cgcggcgcgt tcgcgcaccg cgaccccgat cgcgcacagc gagtcgaaca
     1681 ccgcggccag cgcccgatcg cggccgtcgc ggtccagcag ttcggatatg cgcccaccca
     1741 gcagcggcag gcgacgcgcg gcgcgtggca gcgaggcggc aatcaacagc ggaacgtagc
     1801 cggtgtcgtt gccaccgatg gtgattgtga ccaggctctc cgagccgtcc agcgcggcga
     1861 gctgggccgg ctcgccgtgt tgacgctcgg cgagcacatg ggcggtggtc gcaccggaaa
     1921 atgtgacgtc gaccagttcg aggttccgtc gatcggcgac cagatgcgcg tagttgtggg
     1981 ccgatcgacc cgaccatcgc ggagctcctg cggcgcgcgg ccggataccg ggaccggcgg
     2041 ccatcgaact ccccagcgcg acataacgtt tcatcgtcgc cgggccgcgg atcaattgcg
     2101 cggcttcgct gggctggggc tcaattgccc ggctctcccc cgcaagcggg aggtgccccc
     2161 acaacgggcc gctgcgcggc ccgcatcgtc gccgggctgg aggcctgcgc ccagaaccgc
     2221 ttcgggatgc gtccggcccg ccgcgccaga tgcccggcgg tgatagcggc ggccatcgcg
     2281 gcggccatgg ccggcgggtc ggcggcgcgg gttaccgcgc tggccagcag cacggcgtcg
     2341 caacccagct ccatcgccag cgcggcatcg ctcgcggtac cgatgccggc gtcgagcacc
     2401 accggaacac cggcccgggc gacgatcatc tcgatgttgt gcggattgat gatgccaagg
     2461 ccggtaccga tcggtgaacc cagcggcatc accgccgcgc aaccgatgtc ctccagccgg
     2521 agcgccaaca ccggatcgtc gttggtgtag ggcagaacga cgaatccgtc gtcgaccaat
     2581 tgctctgccg ctctgaccaa ttcgaccgca tcgggcagca gcgtgcgttc gtcggcgatc
     2641 acctcgagct tgacccagtt ggtgctgagc gcctcgcggg cgagttgcgc ggtgagcacc
     2701 gcctcggccg cgctgcggca gcccgcggta ttgggcagtg gcgtgatgcc gagtcggttg
     2761 agcaggtcca gcaatccggt cccgccctct gcgtcgaccc ggcgcatggc gacggtggtc
     2821 agctcggtgc ccgacgcgat cagtgcctct tccagcaccg cgaggctggt cgctcccccg
     2881 gtacccatga tgagccgcga ggcgaagctg cggtcagcga tggttagttt tgagtcagcc
     2941 accctgcacc gccgtcacca cctcgagtcg taccggccgg ccagaaaagt ccaaaagctt
     3001 tgtcgtccaa tccgaacgcg gcagcacgga aaagtctatc gccaccgcga tgccgcggtc
     3061 cgggaaaccc agcgactgca gcaacgcggc gacggtggtc tgctcgtcga cctcgatctg
     3121 tttgtcgttg accatgacga tcacgctcgg gctccgatcg ggacgagttc ggaaacaatc
     3181 tgttcggcgg tccatggcgc caacaggaac cccgaccggc cgtggccggc ggcgaccagg
     3241 gttcgcgcgt ccagacgttg caccagtggg agattatcgg gcgtcatcgg gcgcagcccg
     3301 gcggcgcact cggccagctc gtactcgccc aatgcgggca gcaccacgca cgcgtcgtca
     3361 agcaggtcac gcacccccga taccaccggg gccgtgtcgc gaccgtgttc gtattgggtt
     3421 gcgccgacga ccacgccgtc cgcgcgcggc accagataca cctgccgtcc gtgcacccga
     3481 gcgcgaatta ccctctgcag caacggcata caccccttcc gccagcgcag acgcagcacc
     3541 tcccccttca ccgggcgcac cggcaggccg ggccacagcg ccggcgcgtc aatgccgttg
     3601 gcgatcacca ccgcgtcacc gtccacgcaa gaaaggtctt gcaccggcgc cgcccaccgg
     3661 actccgagac gttcgcaggc caggctcagc gcctcgagca gcgcgcggtt gtccaccgcc
     3721 agttcggtgg gcgcccggaa gccgtgccgg atgccctgtg ccagcagcgg ttccacgtcg
     3781 cgcgcggccg actcccacac caccggatgc ccttgctcgg acagccagtc ggccatggtg
     3841 cgcaggtctg cgacgtcggc ccggtcgacg gccaccacca gcgactcgcg ggcggtgacc
     3901 acctctggcg gcagcccgtc caagaagcca ccctcgcgcc agagccgcag agactccagg
     3961 ccgagacgca acaaccgctc ttcacccggc cagccctcgc tgtgcggcgc cagcatgccc
     4021 gcggccaccc aggatgcgcc cggctcgccg ctgcgatgga cccgcacgga ccaaccggcc
     4081 tgcgccgccc ggcgcgcgac cgacagcccg atgacgccgc cgccgatgac ggccagcaac
     4141 ccggtcggtg tgggcgccgg catcccgggc atttgcggtc ctcccttcgc cggcatgatc
     4201 cggatcaggt gtgacggtaa ggaccggtcc aggtgccctg gctgtgtcca ctctcagccc
     4261 cgcagatgac gaccacccgg gactcccgtg tctgaagttg tgctccggct ctacgctagc
     4321 gcgctagcgt cgcggtgtgc acgtgtctct tggccggccc gccgcccgtc tcgccgcagc
     4381 gcggctgtac ctgtgtaccg acgcgcgccg cgagcgcggc gatttggccc agttcgtcga
     4441 cgccgccctg gccggcgggg tagacatcgt ccagctgcgc gacaaaggat cggccggtga
     4501 gcagcggttc ggcccgctcg aggcgcgcga cgagctggcc gcctgcgaga tccttgccga
     4561 tgcggcgcgc cggcacggcg ccctgttcgc ggtcaacgac cgcgccgata tcgcccgcgc
     4621 ggccggcgcc gacgtgctgc acctgggcca gggcgacctg ccgccgggcg tggcgcggga
     4681 gatcgtcggc ccggacatgg tgatcgggct gtccagccat gaccagaccc aggccaccgc
     4741 ggccgcgacg gggatggccg actacttctg cgtcggcccc tgctggccca cccccaccaa
     4801 acccggtcgc cgggcgccgg gcctggagct ggtgcgcgcc accgctcaat tggtctctgc
     4861 gctcggcggc gacaggccgt ggttcgcgat cggcggcatc gacgcgcggc ggctgcccga
     4921 ggtgctcgac gccggcgccc ggcggatcgt ggtggtgcgg gcgatcaccg cggccgagga
     4981 cccgagggcc gcggccgagc aactcaggtc ggcgcttgca gcagcgagtt gatgcggaga
     5041 ctcagcgggg agggcgcctg gtcggccgga agtggggggc cgggcataca ccaaacgaaa
     5101 accccggtct cgacctcaac cgtgcgcggc agtcgctggc gtcgttcgtc gccgtcgccg
     5161 agcggcacca gcgcgggagc cgtgcgcagc cggtcccagc cggccgcgaa gccgggatgc
     5221 agcggcaggt cggccacctc gtcctcgccg acccagcgca gctcggtgct ctcccggttg
     5281 ggcacggtgt gcagcagctc ggccgcgtcg gcgacgacgg tggtgtaggt ccagtgggca
     5341 ccgccgatcc cggcgacctc agcggtgacg accgcgccgc gcacggcgaa tcgctcgacc
     5401 gggaggccgg cctcttcgcc tgcttcgcgg accgcggttt gttccggggt ctcgtggctg
     5461 tcccgggcgc cgccgggcaa agcccaggtg ccaccctgat ggctccacca cgcccgatgc
     5521 tgcagcagta ccgcgggggt accgtcaggc cggggggcac gcaacagcag gccggccgcg
     5581 ccgtaccggc cccagtagtg agcgccgctg tcggatatca cccatccgtc accgtcgccc
     5641 tgcacgtgtt caggatatgc aggcgatccc gcgggtcaat taggtccggc ttcccccatc
     5701 ggcaggaaga ttctcttaga attcgcttat tcgggttcgt acagcgaatt gcatgcgttt
     5761 cagaggtcga aagagtagat gaggtctgat cagacgtgac ggttgagctg gcgcacccgt
     5821 cgaccgagcc gaaagggtcc cggtcgccgg ccgaacccgc ccatccccgc tggtggttca
     5881 tttcgacgac gccgggccgc atcctgacca tcgggatcgt gctggcggcc ctcggcgtct
     5941 ccagcgcctt cgccacctcg acgacgatca accaccgcca gcaagtgctg accacggtgc
     6001 tcaaccacac cgagccgctg tcgttcgccg ccgggcggct ctacaccacg ctgtcggtgg
     6061 ccgacgccgc ggcggcgacc gcgttcatcg cccaagccga gccgcagacg gtccggcagc
     6121 gctacgagca ggccatcacc gacgccgccg aagccgtgac cagagcgtcc agcgggctga
     6181 cggacgagcc ggcggtgcaa ctgctgggcc ggatcaacgc cgaattggcc gtctataccg
     6241 ggctgatcga aatcgcgcga accaacaacc gggagggcaa cccggtcgga tcgtcgtacc
     6301 tgtcggaggc atcggggttg atgcaatcga cgatcctgcc cgacgcggca cagctctatc
     6361 aggcgacgtc ggagcgggtg gatacggaaa ccaccgcgtc cacccatttc ccggctccgg
     6421 tgatcctcgt cgtcgccggc acgctggtct tcggcgcgtt ctcccatcgc tggctggccc
     6481 ggcgtacccg gcgcagggtc aatccgggcc tggtcgtggg cgctctcggt atcctcgtca
     6541 tggtggtatg ggtcggaacc gcgctgacca tctcaacgac cgccagccgg agcgccaaag
     6601 acaccgcggc ggagtcactt aagacggtga cgaacgtcgc catcaccgcc cagcaggcac
     6661 gagccgacga aaccctgtca ctgatccgcc gcggcgacga agagatccgc aagcagtcgt
     6721 tttatcagcg catcgactcc atgcaccagc agctcgacga atacatggcg cgcagcgacg
     6781 ccgtcgacaa gcccgacctg cagggcgccg atcagttgct ggtccgctgg cgccaggcca
     6841 acgaccggat caactcctac atctcggtgg gcaactaccg ggccgccact caggtcgcgc
     6901 tgggcagcgg cgaggacgac tccaccccgg ccttcgacaa gctcgaagac cagttaggca
     6961 aggccatgga ccaaagccgc acccacctgc gcaacgacgt cctgaacgcg cgcagcgggt
     7021 tgtccggcgc ccaggtcggc ggcgtggtcc tcagcctcgg cgccgcgatc gcggtcgcgc
     7081 tgggcctgtg gccccggctg aaagagtatc gctgatggcc gggctgccgc gcctgcccag
     7141 gttccgtccg gcgagctacc gtctcggggt gttcgccgcg gcggtcgtac tggcgggttg
     7201 cagccgcacg gagtcactga cggtcgccac ggtgccgacg ctgccgccgc ccacaccgat
     7261 cggcatggaa ctgctgccgc cgcagccgcc gctgccggcg gacgacgcca gccaggactg
     7321 caatgcgacc gccagcctgc gccccttccc caccaaagcc gacgcggacg ccgccgtcgc
     7381 cgacatccgc gcccggggca ggctgatcgt cgggctcgac atcggcagca acctgttcag
     7441 cttccgcgac ccgatcaccg gcgagatcac cggtttcgac gtcgacatcg ccggcgagat
     7501 cgcgcgcgac atcttcggcg ccccgtcgca tgtcgagtac cggatcctgt cgtccgacga
     7561 gcgcgtcacc gcgctgcaga agtcggaggt cgacgtcgtg gtcaagacga tgaccatcac
     7621 ctgcgagcgc cgcaagctgg tgaacttctc caccgtctac ctcgacgcca gccagcgcat
     7681 ccttgtcccg cgcgactcgc cgatcagcag ggtggccgac ctgtccggca aacgggtctg
     7741 cgtggccagg ggcaccacgt cgctgcaccg gattcagcag atcgacccgc cgcccgacat
     7801 cgtctcggtg gtcagctggg ccgactgcct ggtggcgatg caacagcggg agatcgacgc
     7861 cgtcagcacc gacgactcga tcctggccgg cctggtcgag gaagacccct acctgcacat
     7921 cgtcggcccg aacatggcca cccagcccta cggcgtcggc gtcagcctga acaacaccgg
     7981 actggtccgg ttcgtcaacg gcaccctgga acgcatccgc cgagacggca cgtggaacac
     8041 gctgtaccgc aagtggctga cggtgctggg ccccgcgccc gccccgccca cgccgaggta
     8101 tgtggactga tggccagacc gatggacgct aaaggcgcgg cagacgccaa agaccctgaa
     8161 gacctggctc cgggcaccca gcctgccgac gtgcagtcgg gcccgctgtc ggaggggcgg
     8221 ccacaggcta cccaggcgct gttccgcccc gatttcgacg acgatgacga cgacttgccg
     8281 cttttcaccg gcgccgccat agatacagaa ccgcaggatc gaatgacggt ggccacgcgg
     8341 gtactcccgc cgatcaggca gctcggcggt ggcctggtcg agatcccccg gatgcgggac
     8401 atcgacccgc tcgaagccct gatgaccaac ccggtggtgc cggagtccaa gcgcttctgc
     8461 tggaactgcg ggaagccggt cggccggtcc ggctcccagg acagcgagga cggcaaggga
     8521 tcttcagagg gctggtgccc ggcctgcggg agtccttact cgttcctgcc ccaactgaat
     8581 cccggggaca tcgtggccgg ccagtacgag gtcaagggct gcatcgcgca cggcgggctg
     8641 ggctgggtct acctggcggt cgaccacaac gtcaacgacc ggccggtggt gctcaaaggc
     8701 ctggtccact ccggggacgc cgaggcgcag gccatcgcga tggccgaacg ccagttcctc
     8761 gccgaggtgg tccacccgca gatcgtgcag atcttcaact tcgtcgagca caccgaccgg
     8821 cacggggacc cggtcggcta catcgtcatg gagtacatcg gcggccagtc gctcaagcgc
     8881 agcctcaaaa acgggaaagc cgagaagctg ccggtcgccg aggccatcgc ctacctgctg
     8941 gaaattctgc cggcgctgag ctacctgcat tccatcggct tggtctacaa cgacctgaag
     9001 ccggagaaca tcatgctcac cgaggagcaa ctcaagctga tcgacctggg tgcggtgtcg
     9061 cgcatcaatt cgttcggcta cctctacggc acacccggtt tccaggcacc ggagatagtg
     9121 cggaccggcc cgacgatcgc caccgacatc tacacggtgg ggcgcacgct cgcggcgctg
     9181 acgttgaacc tgcgcacccg caacggccgc tacgtggacg ggctgcccga agacgacccg
     9241 gtgttaggca cctacgactc gtttgcgcgg ctgctgcgcc gcgccatcga ccccgacccg
     9301 cggcgccggt tctgcagcgc cgaggagatg tccgcccagc tgatgggcgt gttgcgcgag
     9361 gtggtcgccc aagacaccga cgtgccgcgt cccggcctgt cgacgatctt cagccccagc
     9421 cgctcgacat tcggggtgga cctgctggtc gcgcacaccg atgtgtacct ggacggccag
     9481 gtccactcgg agaggctgac ggcaagggaa atcgtgaccg cgctgccggt gccgctggtc
     9541 gatcccaccg atgtggccgc cccggtgctg caggcgacgg tgctctccca gccggtgcag
     9601 acgttggact cgttgcgcgc ggcccggcac ggttcgctgg acgccgacgg catcgagctg
     9661 tccgagtcga tcgagctacc gctgatggaa gtccgcgcgc tgctcgactt gggcgacgtg
     9721 gccaaagcca cccgcaaact cgacgacctg gccgagcggg ccggctggcg gtggcggttg
     9781 gtctggtacc gggccgtcgc cgagctgctc accggcgact acgactccgc cacaaaacat
     9841 ttcactgagg tgctggacac cttccccggc gagctggggc ccaaactggc cttggccgcc
     9901 accatcgagc tggcccgcaa caccgacgag cactggttct accagacggt gtggaagacc
     9961 aacgacggcg tgatctcggc ggctttcggg ctggccagat ccctgtccgc ggagggcgat
    10021 cggaccggcg cggtgcgcac cctggacgaa gtgccgccca cctccaggca tttcacgacc
    10081 gcgcgcctga ccagtgcggt caccctgatg tccggccggt ccacgggcga aatcaccgag
    10141 gcacagatcc gcgacgcggc ccggcgggtg gaggcgctgc cgcccaccga accacgcgtg
    10201 ctgcagatcc gtgccctggt gctcggcggc gcgatggact ggctggaaga cggccacgag
    10261 gccagcacca accacatcct cgggttcccg ttcaccgaac acggcctgcg actgggtgtc
    10321 gaggcgtcgc tgcgcagcct ggcccgggtt gcgcccacgc agcggcaccg ctacacgctg
    10381 gtggacatgg ctaacagggt gcggccgacc agcacgttct agggccttgc gggtcgagtg
    10441 tgagcgcagg gctttcgctg tgagcccacg actttcggtg tgagcccagg gtggaattcc
    10501 gacccgattc gcgccgccag tacacaccca aagccatcag tacacagtcg aacgcctgcc
    10561 ggccaacgcc tctcggacgc ggcgcaagat attggcgggg cggtcctccg caacgacccg
    10621 gatgacgatc catcccatct gctccagcat gtcgattcgc cgaatgtctt tcacaaattg
    10681 ccgtcggtcg gtccggtgtt ggtcaccgtc gtactcgaca gcgaccatgt actccgccca
    10741 gcccaggtcg aggtaggcca ccggcatgcc gtcgacaccc tgcaccggta tttgggtctg
    10801 cggccgaggg aggcccgcgt cgatgaggag cagccgcagg tagctttccc gcggcgactg
    10861 tgcgcccgcg tcgaccagca ccagcgcgga ctccagtcgc cgcagcccgg gcgagcgggg
    10921 gtggcatttc gcgatccgca acacgtcatc gaccttgaaa cccgttgccc gcgccagcgc
    10981 gtccagccgg acgaccgccg agcggacggc cccgcgccgg ccgatgtcga aggccgttcg
    11041 ctcgggtgtg gagacgggac gcccggcgat ggtctgcgtc tcgccgtcca gcaacacatc
    11101 gcgccgcgtc agcacgccct gaggtggtcg ggaattgttg tatagcagct caaccggaac
    11161 atcctcgggg atccaccggg cgccgtgtaa tgccgcggcg gccgccccca cgatgatcgc
    11221 cgtccgtttc gaccacagcc aggcggcgaa aatccggagc tccagagaca gctggacgtc
    11281 gttggacagg tagacgttcg gaagtaccgc gcggtagcgc gtccgcagtt gatgccggtt
    11341 tatcgttccg gacgccagtg cctcgctgcc gatgaacggt tctctcgcat cccgcatacc
    11401 gcagaattgc atgccgacgg ctgggcgcga tttcgcctct ccacagggcc cggcccggac
    11461 cgcaagcaaa tcgcaagaaa acccgggcac tgttgattca tgccggggca gtccatgcag
    11521 tgggtcacca cggcccgcat gctgcgccgg gccgggttcg gggtgaccgg acccgaggtc
    11581 gacgcggccg taacccggga ct
//
LOCUS       DASSGQ010000312         4977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1298396,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000312 DASSGQ010000000
VERSION     DASSGQ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298396"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VFI55_13775"
     CDS             <1..1180
                     /locus_tag="VFI55_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PPE family protein"
                     /protein_id="HET7075136.1"
                     /translation="LGNSGSFNWGFGNTGNGNVGLANTGNGSIGIGLTGDHQVGFGGW
                     NSGSGNSGLFNSGTGNSGFFNSGSNNVGIANTGGQNWGMLNSGTLNTGIGNTGSIDTG
                     LWNAGSTDAGLFNPGSNNVGLGNAGTYNLGGFNAGGANPDLGFSGGENVGFFNAGNFN
                     MGSFNAGIANLGWSNAGTHNVGAFNSGFFNTGLWNSGTTNTGFFNSGDANTGLWNSGN
                     TNTGLWNSGNVNTGVGGAGDINAPNSGFGNSGTGNSGFFNSGTGNSGGYNTASGGGNS
                     GFENSGSGGFNVGFYNSAGNGYDVGFYNSGSDGYDVGFYNSGSGSENVGYANSGSFNT
                     GFMNGGVGLNTGFYNSGAGGNAGWGNSGAGDSGFGNSGSTNSGGNNAGTGQSGFFNPA
                     "
     gene            complement(1322..1395)
                     /locus_tag="VFI55_13780"
     tRNA            complement(1322..1395)
                     /locus_tag="VFI55_13780"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1359..1361),aa:Arg,seq:tct)
     gene            1582..2826
                     /locus_tag="VFI55_13785"
     CDS             1582..2826
                     /locus_tag="VFI55_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7075137.1"
                     /translation="MSVVLNVASRCSRAGAQALQLAADARDTCQAGALLLRGSPSAVG
                     WFAGWLSTEFPPHVVTGHALCRVSPPSIARVATAWAAQRADQALTAGLEECFGPDFRE
                     LVRQPAYAQSECTPRAGLLRMPGPHRRYAAETSNIPYGPGGSAHLLDIWRRHDLAPGY
                     RAPVLIQVPGGAWTLNRKRGQAYTLMSRMVELGWICVSINYSKSPRCAFPAHLIDVKR
                     AIAWVRENIADYGGDPDFIAITGGSAGGHLASLAALTPNDPAFQPGFEDADTTVEAAA
                     LYYGVYDFTDLENMNDIMLPFLEHFVLKARYADEPERFRAASPISYVHRDAPPFFVLH
                     GEKDELVPSGQGRAFCAALREAGATVGYAELANAHHAFDITPTVRSKLAADAVADFLG
                     VVYGRRGGSLRAVLPLSASSAS"
     gene            2987..4462
                     /locus_tag="VFI55_13790"
     CDS             2987..4462
                     /locus_tag="VFI55_13790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HET7075138.1"
                     /translation="MSESIETAKLSDELGPVDYLMHRGEANPRTRSGIMALELLDTTP
                     HWERFRTRFENASRRVLRLRQKVVIPTLPTASPRWVVDPDFNLDFHVRRVRVSGSGTL
                     REVFDLAEVILQSPMDISRPLWTATLVEGLADGKAATLLHVSHAVTDGVGGVEMFAEF
                     YDLERDPPPKPTPPQPIPQDLTPNDLMRQGINRLPFAIVNSVVGAVSGAVSAAGKAVM
                     DPVSTVGGAVGYAMSGVRVINRAAEPSPLLRRRSLATRTEAIDIRLADLHKAAKAGGG
                     SINDAYLAGLSGALRRYHIALGVPISTLPMSVPVNLRAEADTAGGNRFTGVNLAAPLG
                     TADPVSRMKKIRAQMTQRRDEPAMNIIGYMAPVLSVLPTPVLEGITGSVIGADVQASN
                     VPVYPGDTYIAGAKILRQYGIGPLPGVAMMVVLISRGGWCTITVRYDRASVRDEPLFA
                     QCLLEGFDEILALAGRPEPRAVPASFVAERAGSTSRSVSDS"
     gene            4459..>4977
                     /locus_tag="VFI55_13795"
     CDS             4459..>4977
                     /locus_tag="VFI55_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HET7075139.1"
                     /translation="MSAAQEDERGSKKSGKPPADMRLPGSVAEIVASPPGPRIGAFFD
                     LDGTLVAGFTAVILTQERLLRRDMGVSELLGMVQAGLSHSLGRIEFEDLIGKAAAALT
                     GRLLDDLDEIGERLFAQRIEGRIYPEMRELVRAHMARGHTVVLSSSALTIQVDPVARF
                     LGIPNTLTNKFET"
BASE COUNT          853 a         1650 c         1644 g          830 t
ORIGIN      
        1 cttggggaac tccggcagct tcaactgggg cttcggcaac accggtaacg gcaacgtcgg
       61 cttggcgaac accggcaacg gaagtatcgg catcgggctc accggcgatc accaggttgg
      121 cttcggcggc tggaactcag gtagcgggaa ttcgggcttg ttcaactcgg gcaccggcaa
      181 ctccgggttt ttcaactcgg gcagcaacaa cgttggcata gcgaataccg gcggccagaa
      241 ctggggcatg ctgaactcgg gcactctgaa cactgggatc ggcaacaccg gcagcatcga
      301 cacgggcttg tggaacgcgg gcagcaccga cgccggtttg ttcaatccgg gctccaacaa
      361 cgtcggcctg ggcaacgccg gcacctacaa cctgggcggc ttcaacgctg gcggcgccaa
      421 ccccgatctc ggcttttccg gcggcgaaaa tgtgggcttc ttcaacgcgg gcaacttcaa
      481 catgggcagc ttcaatgccg gcatcgccaa cctgggctgg tccaacgcgg gcacacacaa
      541 cgtgggcgcc ttcaactcgg gcttcttcaa caccggtttg tggaactcgg ggaccaccaa
      601 caccgggttc ttcaactccg gcgacgccaa caccggtttg tggaactcgg gcaacaccaa
      661 taccggtttg tggaactcgg gcaacgtcaa cacaggcgtc ggcggcgcgg gcgacatcaa
      721 tgcccccaac tcgggcttcg ggaactcggg cacgggcaac tcgggcttct tcaactcggg
      781 tacgggcaac tcgggtgggt acaacacggc cagcggcggc ggcaactcgg gcttcgagaa
      841 ctcgggtagc ggcggcttca acgtcggctt ttacaactcg gctggcaacg gctacgacgt
      901 cggcttctac aactcgggta gcgatggcta cgacgtgggc ttctacaact cgggcagcgg
      961 ctctgagaac gtgggctacg cgaacagcgg cagctttaac acgggcttca tgaacggggg
     1021 cgtcggcctc aacaccggct tctacaactc gggcgccggc ggcaacgcgg gttggggtaa
     1081 ttccggcgcg ggtgactcgg gcttcgggaa ctcgggcagc acgaattcgg gaggaaacaa
     1141 cgcggggacc gggcagtccg gtttcttcaa tccggcgtaa ggcgttgtcc cgctgccgag
     1201 ccggactgac acggatcgac gagaccaaga ccgccaccgg ccgccggacc gtggatcacc
     1261 ccgtgacgga cagaaacgat gaccggcatt gtcggtagga ccgaaaacac cctctgagta
     1321 gtgcccccgg cacgactcga acgtgcgacc tagggattag aaggcccttg ctctatccac
     1381 ctgagctacg gaggcaatgc gcaggtcagt ctatccaacg gcacgagccg aataatttgc
     1441 ggcgacgcga cgcgcccgaa aagtcttccc gcgccgcact tatcggctat ggtgtgagcc
     1501 gcgacacacg tacatcaccg gctgatcatc ggccgttaac cgcaacgagg cgtgtgcata
     1561 acgtcgaggg atggcgtcgg tatgagcgtg gtgttgaacg tggcttcgcg gtgctcgcgg
     1621 gctggtgccc aggcattgca gctggcggcc gatgcccgag acacctgcca ggccggcgcc
     1681 ctgttgctgc gcggctcgcc ctccgcggtt ggctggttcg ccggctggct gtccacggaa
     1741 tttcccccgc acgtggtcac cgggcacgcg ttatgccggg tctcacctcc ttcgatcgct
     1801 cgcgtcgcca ccgcgtgggc ggcgcagcgc gcggaccaag ccctcaccgc cgggctcgaa
     1861 gagtgcttcg ggccggactt tcgtgagctg gtgcgccaac cggcctatgc ccagtccgaa
     1921 tgcactcccc gcgctgggct attacgcatg cccggcccgc accgcaggta cgcggccgag
     1981 acctccaaca tcccctacgg tccgggcggc agcgcacacc tgctcgacat ctggaggcgc
     2041 cacgacctgg cgcccggcta tcgcgcgccg gtgttgatac aggtccccgg aggggcgtgg
     2101 accctgaacc gcaaacgcgg tcaggcgtac acgctgatga gccgcatggt cgaactcggc
     2161 tggatctgcg tctcgatcaa ctacagcaag agcccgcgat gtgcgtttcc ggcgcacctc
     2221 atcgacgtga agagggcgat cgcgtgggtc cgcgagaaca tcgccgacta cggcggcgac
     2281 cccgacttca tagcgatcac cggcgggtcg gcgggcgggc acctggcgtc gttggccgcg
     2341 ctcaccccga acgatccagc cttccagccc ggatttgaag acgctgacac gaccgttgag
     2401 gcggccgcgc tctattacgg cgtctatgac ttcaccgacc tcgagaatat gaacgacatc
     2461 atgctgccgt tcctcgagca cttcgttctc aaggcccgct acgccgacga gccggagcgg
     2521 ttccgggcgg cgtcgccgat ttcctacgtc caccgcgacg ccccgccgtt ctttgtgctg
     2581 cacggcgaga aggatgagct ggttcccagc ggccagggcc gggccttctg cgcggcgcta
     2641 cgtgaggccg gagccacggt gggctacgcc gaacttgcca acgcccacca cgcgttcgac
     2701 atcaccccga cggtccgctc gaagttggcc gccgacgccg tcgccgactt cttgggcgtc
     2761 gtctacgggc ggcgcggcgg ctcgctccgg gccgtgttgc cgttgtcggc aagctctgcc
     2821 agctgaccca ggcctctgcc ccggaccctc gtgcccgggt tccggactcg accgcgcctt
     2881 tcaccagcgc cgcaacccga ctagcgttgg gttggaattg ggttggcaag tccaaggcag
     2941 gaggcttggt cggcggtgac aacgttggcg catcgagtta acgatcatgt ctgagtccat
     3001 cgagaccgct aaattgtccg acgagcttgg accggtcgac tatctgatgc atcggggtga
     3061 agcgaatccc cggaccaggt cggggatcat ggcgctggaa ctcctcgaca ccacgccgca
     3121 ctgggagcgg ttccgtaccc ggttcgaaaa cgcctcccga cgagtattgc ggctgcggca
     3181 gaaggtcgtc ataccgacgt tgccgacggc gtcgccgcgt tgggtggtgg acccggactt
     3241 caacctggac ttccatgtgc gccgggtacg cgtctccggg tccggcacgc tgcgtgaagt
     3301 tttcgatctc gccgaggtga tcctgcagtc gccgatggac atctcgaggc cgctgtggac
     3361 ggccaccctc gtcgagggtc tggccgacgg caaggcggcg acgctgctgc acgtcagcca
     3421 cgcggtcacc gacggcgtcg gcggcgtcga gatgttcgcg gagttctacg acctcgagcg
     3481 cgatccaccg ccgaagccga cgcccccgca accgattccg caggatctga cgcccaacga
     3541 cctgatgcgg cagggcatca accggctacc cttcgccatc gtcaacagcg tcgtgggcgc
     3601 ggtctccggg gcagtatcgg cggccggcaa ggccgtcatg gatcccgtct cgacggttgg
     3661 gggagccgtt ggctacgcca tgtcgggcgt ccgggtgatc aaccgggccg ccgaaccgtc
     3721 gccgttgctg cgccggcgca gccttgccac ccgcaccgaa gcgatcgaca tccggctcgc
     3781 cgacctgcac aaggccgcaa aagccggcgg gggatcgatc aacgacgcct acctcgccgg
     3841 cctgtcgggc gcgttgcggc gctaccacat cgcacttggc gtgccgatca gcacgctgcc
     3901 gatgtccgtg cccgtcaacc tgcgggccga agccgacacg gccggcggca accggttcac
     3961 cggcgtcaac ttggcggcgc cgttgggcac cgccgacccg gtctcgcgga tgaagaagat
     4021 ccgcgcgcag atgacgcagc gccgcgacga gcccgcgatg aacatcatcg gttacatggc
     4081 accggtgctg agtgtgttgc ccacacccgt gctggagggg atcacaggtt cggtgatcgg
     4141 cgccgacgtg caggccagca atgtccccgt ctacccggga gacacctaca tcgccggcgc
     4201 aaagatcttg cggcagtacg gtatcggccc gctgcccggc gtggcgatga tggtggtgct
     4261 gatttcgcgg ggcggctggt gcaccatcac cgtgcgctac gacagggcgt cggtgcgaga
     4321 cgagccgttg ttcgcccagt gcctgctgga gggtttcgac gagatcctgg cgctcgccgg
     4381 tcgcccggaa ccccgggcgg tgccggcgtc attcgtcgcg gagcgggccg gctcgacgtc
     4441 tcgatcggtg tcggactcat gagcgccgcg caagaagacg agcgcggtag caagaaatcc
     4501 gggaagccgc cggcggatat gcggttgccc ggttcggtcg ccgagatcgt cgccagccct
     4561 ccggggcccc ggatcggcgc atttttcgac ctggacggga cgttggtcgc gggcttcacg
     4621 gcggtcatcc tgacccagga gcggctgctg cgccgcgaca tgggtgtcag cgaactgctc
     4681 ggcatggttc aggccggcct tagccattcg ctcgggcgca tcgagttcga agacctcatc
     4741 ggcaaggccg ccgcggcgct gaccgggcga ctgctggacg acctggacga gatcggcgag
     4801 cggctgttcg cccaacggat cgaggggcgg atctacccgg aaatgcgcga gctggtgcgg
     4861 gctcacatgg cccgcggcca cacggtggta ctcagctcgt ccgcgctgac cattcaagtc
     4921 gatccggtgg cgcgcttcct cgggatcccc aacacgctca ccaacaagtt cgagacc
//
LOCUS       DASSGQ010000313         3573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1207913,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000313 DASSGQ010000000
VERSION     DASSGQ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207913"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2119
                     /locus_tag="VFI55_13800"
     CDS             <1..2119
                     /locus_tag="VFI55_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET7075140.1"
                     /translation="GNPFLVSELVGGLGEEGRLNVSGGRAVATGEALPRRLGAGMDQR
                     LDHLSEGASEVVRVAAVLPDRFSAGLLAAMLERQPSSLMSALEEAVRADLLVEDGEQL
                     RFGHDLLREATRQSLPQSLRRAMERQSASIMLGMGVAPAEVATQLARSAEPGDREAIS
                     ALRQAAQSVGHSDASAAADLSKRALELLAADDTEHGLLVAETVELLNRASRYEEAEEL
                     AVVALSEVASPQEEAEIRLRLPTLNKHSTQQRVEENRRALQLRDISEVTRARHLAWLA
                     YNLMLNDQRGQRRAAANEAAAAAAATGDLESKIVANLTLALLDDGEGYAGRSLHRLEE
                     LCALARTSDATAVHDLAAYPYAFLLAVVGRLDDAAAQIADGVEQARREGNAMALAIWA
                     TIEGVVHLTAGRLSAARAAIESLPRPQRTAVTELDMIRMVILAEVAARTDDRTLLQQM
                     VNDARDAYSTGSHAVRRGTAHVLALAAWQRDDVQDATRWLGDITLFGSPLWPQVLDQV
                     ILGARVASAAGDAGLRARVLQAIDVLERERPAIPLFTGVAKYARGVLERDAQALLAAA
                     AVLHMSSRPLLYAVAAEDAGAELARTDHLGEALDQLNAAFDTYMHHEALVDARRVGRE
                     LRRLGVQRRIVSQQRAKTGWDSLTDSELTVVNLIAQGATNRDVAEQLHLSLHTVKTHV
                     HNAFTKLGINSRTQLTQLMRGSD"
     gene            2242..3075
                     /locus_tag="VFI55_13805"
     CDS             2242..3075
                     /locus_tag="VFI55_13805"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HET7075141.1"
                     /translation="MVMTILEVGALPKPLRQELAVKYQTLQLPNDASRPAFLAEHGAS
                     VTVVVVGPPGMDAAVIDALPNLGAIVHFQDSHASGDVDEAPQLGIAVSHSPNAFNYGV
                     AETAVGLMLDMVRGFCADGFGRVRRGLGQLVPPLAHQVSGLRVGLLDLSRIGSAIASR
                     LAVELAVSVDALMTAMAGGKGTDKLIDRAVLEALGPDGYLVINTASGGIVDPGADESD
                     ALRQWRAFDNGALFPHIADATRDDWPTADALVLGNLDEYFKTLTTPVVNPTHPPSGTS
                     A"
     gene            complement(3143..>3573)
                     /locus_tag="VFI55_13810"
     CDS             complement(3143..>3573)
                     /locus_tag="VFI55_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011596091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7075142.1"
                     /translation="MPDRPFMLVGQQAVADPTRSTGGLQPVYMYAHVPHGYTGDATES
                     MLVQIERFAPGFRDRIEAVVRRTPDDLERDNPNNIGGDINGGAMDIAQFIARPRLAVN
                     PYWTGVDGHYLCSSSTPPGGGVHGMCGHLAAQAALRRTRQ"
BASE COUNT          583 a         1128 c         1256 g          606 t
ORIGIN      
        1 cgggaacccg tttttggtca gcgagctggt ggggggtctg ggcgaggagg gccggctcaa
       61 tgtgagcggc ggccgggcgg tggccactgg ggaggcgctg ccgcgccggc tgggcgccgg
      121 tatggatcag cgactcgacc acctctccga aggtgcgagc gaggtggtgc gggtggccgc
      181 ggtgttgccg gaccggtttt cggcggggtt gctggccgcg atgctggaac gccagccgtc
      241 gtcgttaatg tcggcgctcg aggaggcggt gcgtgcggac ctgctcgttg aagatggtga
      301 gcagttgcgg tttgggcatg acctgctgcg ggaggccact cggcagtcgt tgccgcagtc
      361 gctgcggcgt gcgatggagc gccagtcggc gtcgatcatg ctggggatgg gcgtggcccc
      421 agccgaggtg gccactcagc tggcgcgcag cgccgagccc ggagaccggg aggcgatcag
      481 tgcgctgcgg caggcggcgc aatcggttgg gcacagcgat gccagtgcgg ccgcggattt
      541 gagcaagcgc gcattggagc tgttggccgc cgatgacact gaacatgggc tcctggtcgc
      601 cgaaacggtg gagttgctca accgggccag ccgctacgag gaggccgaag agctggccgt
      661 tgtggcgctc tcggaggtgg catcgccgca agaggaggcc gagattcgtc tgcgcctccc
      721 gacgctcaat aagcacagta cccagcagcg cgtggaggag aatcgtcgtg cgctgcagct
      781 gcgcgacatc agcgaggtca cccgggcacg gcatctggcg tggctggcct acaacctgat
      841 gttgaacgac caacgtgggc aacggcgcgc ggcggccaac gaggcggccg cggccgccgc
      901 cgcgaccggt gatctcgagt cgaaaatcgt ggccaacctc accctcgcct tgctcgacga
      961 tggggagggg tacgcgggcc gctcactaca ccgcctcgag gagctctgcg cgctcgcccg
     1021 cacgagcgac gcgaccgccg ttcatgactt ggccgcatac ccctacgcgt tcctgctggc
     1081 ggtggtcggc cggttggacg acgctgcggc ccagatcgcc gatggagtag agcaagcgcg
     1141 ccgggagggc aacgccatgg ccctcgcaat ctgggcgacg attgaggggg ttgtccatct
     1201 cactgcgggc cggttgtctg ctgcgcgcgc cgcgatcgaa tccctgccgc gcccacagcg
     1261 gacagccgtg accgagctgg acatgatccg gatggtgatt ctggccgagg tggcggcgcg
     1321 caccgacgac cgaacgttgt tgcagcagat ggtcaatgac gcgcgcgacg cctactccac
     1381 tggctcacat gcggtgcgac ggggcacggc gcacgtgctg gcgctggcgg cgtggcagcg
     1441 cgacgacgtc caggacgcga cgcgctggct gggcgacatc accctgttcg gctcaccact
     1501 ctggccccag gtcctagatc aggtgatctt gggtgcgcgg gttgcgtcgg cggccggcga
     1561 tgccggcttg cgcgcgcgtg tgctgcaggc cattgatgtg ctcgagcgtg agcggccggc
     1621 gatcccgttg ttcaccgggg tggcaaagta tgcccgcggg gtcctcgaac gtgatgccca
     1681 ggcactgttg gctgcggccg ctgtgctcca catgtcgtcg cggccgcttc tttacgcggt
     1741 ggctgccgag gacgccggcg ccgaactcgc ccgcaccgac caccttggcg aggcgctcga
     1801 tcagctcaat gccgcattcg acacctatat gcaccacgaa gcgctagtcg acgcacgccg
     1861 ggtgggccgc gagttgcgcc ggttaggtgt gcaacgtcgc atcgtcagcc agcagcgggc
     1921 aaaaaccggc tgggacagtc tcaccgactc cgagctcacg gtggtcaacc tgattgcgca
     1981 gggtgccacc aaccgagacg tcgccgagca gctgcacctt tccctacata cggtgaaaac
     2041 ccacgtccac aatgcgttca ccaagctggg catcaactcc cgcacccaat tgacgcagct
     2101 catgcgcggg tctgattgac ccgcccgtca aatatcgcgc ccgcctagta ctagacccat
     2161 ccggccctaa acaccaatcc tcgggccata gcgctgtgca ggcggcgtcg cgaccctacg
     2221 aatgggtggg cagaaagggt gatggtgatg accatccttg aagtaggagc gctaccgaag
     2281 ccgctgaggc aagagttggc tgtgaaatac cagacgttgc aattgcctaa cgacgcttcg
     2341 cgccccgcgt ttctcgcaga gcacggcgct tcggtcacag tcgtcgtcgt cggaccgcca
     2401 ggcatggacg ccgcggtgat cgacgcgctg cccaacctgg gcgcgatcgt gcatttccaa
     2461 gacagccatg ccagtggcga cgtggacgag gcgccacagt tagggatcgc ggtcagccat
     2521 agccccaatg cattcaatta cggggtcgcc gagaccgcgg tcggcttgat gctggatatg
     2581 gttcgcggct tttgcgcgga cggcttcggg cgagtccgac gcgggctggg ccagcttgtc
     2641 ccaccgctag cgcatcaggt gagcggactt cgggtaggcc tcctggacct gagccggatc
     2701 ggctccgcca ttgcttcccg gctggcggtc gagttggctg tctcggtcga cgcgctgatg
     2761 accgccatgg cgggcggcaa aggaacagac aagctcatcg atcgcgcggt cctggaagct
     2821 cttggaccgg acggctacct ggtcatcaat accgcatcgg gcggcatcgt cgacccgggc
     2881 gccgacgaat cagatgctct acggcaatgg cgcgcattcg acaacggggc gctgtttccg
     2941 catatcgccg atgcgactcg ggacgactgg ccgacggcag acgcgctggt tctcggcaat
     3001 ctcgacgagt acttcaaaac gctgaccacg ccggtcgtga accccacaca cccaccgagc
     3061 ggaacttcag cctgatcgag agatcgacga gtctttctgc gccgaaccgc taggagcatc
     3121 cgcggtcggc atcggtccat ccctactgtc tggttcggcg gagggccgcc tgggcggcca
     3181 ggtggccgca cattccatgc acgcccccgc cgggcggcgt cgaggacgag cagaggtagt
     3241 gtccgtcgac cccagtccag taggggttta cggccaggcg gggccgggcg atgaattggg
     3301 cgatgtccat agcgccgccg ttgatgtcgc cgccgatgtt gttcgggttg tcacgctcga
     3361 ggtcgtccgg ggtgcgtcgc acgacggctt cgatgcggtc gcggaatccg ggcgcgaagc
     3421 gctcgatctg gacgagcatc gattcggtgg cgtcgccggt gtagccgtgc gggacgtggg
     3481 catacatgta gaccggttgc aggccgcctg ttgatctcgt cgggtccgcc accgcctgct
     3541 ggcccacgag catgaacggc ctgtccggca tct
//
LOCUS       DASSGQ010000314        10103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1390416,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000314 DASSGQ010000000
VERSION     DASSGQ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10103
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390416"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VFI55_13815"
     CDS             <1..234
                     /locus_tag="VFI55_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7075143.1"
                     /translation="ATMDRLFHSLRAGAAGDGRDADAALVAAVRMTAATPARAVGLHR
                     VGGLRTGYDANLVVLDHDWQVAAVMARGDWLVN"
     gene            complement(265..1293)
                     /gene="yhjD"
                     /locus_tag="VFI55_13820"
     CDS             complement(265..1293)
                     /gene="yhjD"
                     /locus_tag="VFI55_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HET7075144.1"
                     /translation="MSEPAKPGILDRLRARFDWFDHAMRAYERFDGGNGSFFAAGLTY
                     YTIFALFPVLMVGFAVCGFMLARRPTLLRTIDDHVRSTVSGPLSQQILDLMNSAIDAR
                     TSVGVIGLATAAWAGLGWISHLRAAVTGMWWVQPPDSPGFVRNKISDFIALAGTFLMI
                     LATLVLTALNQPAPMAAVLKWLGIPDYPVFDWLFRGISIFVSLLVSWLLFTWTIGRLP
                     REKVDLVGSMRGGLMAAIGFEVFKQIASFYLQTVLRSPAGAAFGPVLGLMVFSYVTAY
                     LVLFSTAWAATAADDPRAKPVDAPPPAIITPRVELNEGLRTRQTLTAMAMGAVGALAI
                     SRLTRRAR"
     gene            complement(1302..2333)
                     /gene="trpS"
                     /locus_tag="VFI55_13825"
     CDS             complement(1302..2333)
                     /gene="trpS"
                     /locus_tag="VFI55_13825"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET7075145.1"
                     /translation="MSTPTGSRRIFSGVQPTSDSLHLGNALGAVTQWVALQDDWGDDW
                     DAFFCVVDLHAITIPQDPEALRRRTLATAAQYLALGIDPGRSTVFVQSHVPAHTQLAW
                     VLGCFTGFGQASRMTQFKDKSTRQGSDSTTVGLFTYPVLQAADVLAYDSNLVPVGEDQ
                     RQHLELARDVAQRFNGRFPDTFVVPDVLIPKATAKIYDLADPTSKMSKSASTDAGLIN
                     LLDDPALSAKKIRSAVTDSEREIRFDTDAKPGVSNLLTIQSAVTGAGIDKLVEGYAGR
                     GYGDLKKDTADAVVEFVTPIKARVDELLADPTQLEAVLAAGAERADDVAGKTVERVYD
                     RLGFLPRRK"
     gene            2530..3330
                     /locus_tag="VFI55_13830"
     CDS             2530..3330
                     /locus_tag="VFI55_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7075146.1"
                     /translation="MLLHPFLLSQTVWEDVAQQLADTGRYEVFAPTMAGHNGGPSAGT
                     WLLSSAALADHVERQMDELGWQTAHIVGNSLGGWVAFELERRGRARTVTGIAPAGGWT
                     RWSPAKFEVIAKFIVVIPLLALARLFGPRVLHLPFSRRCATFAISASPDGVSERQLSS
                     IVDDVGHCPAYFQLLLKALLMHGLRELALNAVPAHLVICGKDRIVPAPRFSRHFTTCL
                     PDENHQVTTLEGVGHVPMFEAPDRITEVITGFIEQSCPTDSGRRQPAG"
     gene            complement(3339..4568)
                     /locus_tag="VFI55_13835"
     CDS             complement(3339..4568)
                     /locus_tag="VFI55_13835"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HET7075147.1"
                     /translation="MSNEATIKVKGRVVELDGDEMTRVIWKLIKDMLILPYLDINLDY
                     YDLGIEHRDRTDDQVTIDAAYAIKKHGVGVKCATITPDEARVQEFNLKKMWLSPNGTI
                     RNILGGTIFREPIVISNVPRLVPGWTKPIVIGRHAFGDQYRATNFKVDQPGTVTLTFT
                     PADGSEPMVHEVVSIPPDGGVVMGMYNFKDSIRDFARASFSYGLNARWPVYLSTKNTI
                     LKAYDGMFKDEFQRIYDEEFKDKFEAEGLTYEHRLIDDMVAACLKWEGGYVWACKNYD
                     GDVQSDTVAQGYGSLGLMTSVLMTADGKTVEAEAAHGTVTRHYRQYQAGKPTSTNPIA
                     SIFAWTRGLAHRGKLDKTPAVVEFAQKLEEVVIATVEDGKMTKDLAILIGPKQEWQNS
                     EEFLNSIAENLEKALAN"
     regulatory      4709..4829
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00634"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class IV; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00634"
     gene            5009..6385
                     /locus_tag="VFI55_13840"
     CDS             5009..6385
                     /locus_tag="VFI55_13840"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional o-acetylhomoserine/o-acetylserine
                     sulfhydrylase"
                     /protein_id="HET7075148.1"
                     /translation="MSSESSTDSNSETDPTAHWSFETKQIHAGQQPDAATNARALPIY
                     QTTSYTFDDTAHAAALFGLEVPGNIYTRIGNPTTDVVEQRIAALEGGVAALFLSSGQA
                     AETFAILNLAGAGDHIVSSPRLYGGTYNLFHYSLAKLGIDVSFVDDPDDLDSWQAAVR
                     PNTKAFFGETISNPQIDVLDTPGVAAVAHANGVPLIVDNTVATPYLIQPFTQGADIVV
                     HSATKYLGGHGAAIAGVIVDGGTFDWTQGRFPGFTTPDPSYHGVVFAELGPPAFALKA
                     RVQLLRDLGSAASPFNAFLVAQGLETLSLRIERHVANAQRVAEFLEAHDSVLSVNYAG
                     LPSSPWYERAKRLAPKGTGAVLSFELDGGIEAGKAFVNALRLHSHVANIGDVRSLVIH
                     PASTTHAQLSPAEQLTTGVSPGLVRLAVGIEGIDDILADLELGFAAARKYGAPVSESG
                     DPRAVGAF"
     gene            6398..7549
                     /locus_tag="VFI55_13845"
     CDS             6398..7549
                     /locus_tag="VFI55_13845"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HET7075149.1"
                     /translation="MTISDVPTQTLPAEGEVGLVHIGSLTTENGEVIDDVCIAVQRWG
                     ELSPTRDNVVVVLHALTGDSHITGPAGPGHPTPGWWDGVAGPGAPIDTNRWCAVATNV
                     LGGCRGSTGPSSLARDGKPWGSRFPLVSVRDQVEADIAALAALGIDQVAAVVGGSMGG
                     ARALEWIVGHPDRVRAGLLLAVGARATADQIGTQTTQIAAIKADPNWQGGDYYETGRS
                     PDAGMAIARRFAHLTYRGEVELDTRFANDNQGDENLANGGRYSVQSYLEHQGDKLLAR
                     FDAGSYVILTEALNSHDVGRGRGGVGNALRRCPVPVVVGGITSDRLYPLRLQEELAEL
                     LPGCAGLHVVESICGHDGFLVESDAVGDLIRETLDLALDTDTAEGACLR"
     gene            7555..8289
                     /locus_tag="VFI55_13850"
     CDS             7555..8289
                     /locus_tag="VFI55_13850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7075150.1"
                     /translation="MTRSRHDRSLSFGPAAAAYERGRPSYPPEAIDWLLPRGARQVLD
                     LGAGTGKLTTRLVERGLEVVAVDPIPDMLEVLRVSLPETRALLGTAEEIPLEDNSVDA
                     VLVAQAWHWVDPERAIPEVARVLRPGGRLGLVWNTRDERLGWVRELGQIIGSDGDRGR
                     FDLTLSTAFTEPERHQVEWTNYLTPQALIDLVASRSYCITSPTEVRSRTLDQVRELLA
                     THPALANSTGLALPYVTVCIRATVAE"
     gene            complement(8319..8966)
                     /locus_tag="VFI55_13855"
     CDS             complement(8319..8966)
                     /locus_tag="VFI55_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019047807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HET7075151.1"
                     /translation="MAKPQDDHLWALAHAERAALAEDLSGLSAEQWRHGTLCGKWDVE
                     EVVAHLTAAASLNQWKWLRSMLGARLRPDVHNQRRLIEHRGSTPAETLDRFRAVINST
                     TAPSAHIPAYLGEVVVHAQDIRQPLGLVRTPSVDALTPVAAFFARRDFTVASRTRIAG
                     LCLRADDGPFSAGCGALVSGSTLALVMSMAGRPPYLDELNGPGVQLLRSRVQATD"
     gene            9677..>10103
                     /locus_tag="VFI55_13860"
     CDS             9677..>10103
                     /locus_tag="VFI55_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MspA family porin"
                     /protein_id="HET7075152.1"
                     /translation="MVVRRAAILAVCLAMSLTVAPPTAADPDTEPAAAAAEAPDDGRV
                     PSADQVTFNTTDGWTLALGAKDESQVPLAPLTTAVSSREYLSRGTFFASLEGPEQPRG
                     VLEVGYEIGCGIDMSTSNGVTMAGSAGLNPGLGFPLAGGF"
BASE COUNT         1720 a         3366 c         3284 g         1733 t
ORIGIN      
        1 gccaccatgg atcgcctctt ccacagcctg agagccggcg cggccgggga cggccgtgac
       61 gccgacgcgg cactggtcgc cgcggtgcgg atgaccgcgg cgacaccggc gcgtgccgtc
      121 gggctgcacc gggtgggcgg cctgcggacg ggttatgacg ccaatcttgt tgttctggac
      181 catgattggc aagtggcagc cgtgatggcg cgcggcgact ggctggtgaa ctagcccagc
      241 gggccgcgag cggcggccgg tcggctaacg ggcccgacgg gtcagccggg aaatcgccag
      301 cgcgccaaca gctcccatgg ccatcgcggt cagggtctga cgcgtgcgca gcccctcgtt
      361 cagctcgacc cgcggggtga tgatcgcagg cggtggagca tcgacgggct tggcgcgtgg
      421 gtcgtcggca gcggtagcgg cccaggcggt ggagaacagg accagataag cggtgacgta
      481 ggagaacacc atcaggccca gcaccggacc gaacgcggcc cccgccggac tgcgcagcac
      541 cgtctgcaga taaaacgacg cgatttgctt gaacacctcg aagccgatcg ccgccatcaa
      601 accgccccgc atcgagccga ccagatcgac cttctcgcgc ggcaatcggc cgatcgtcca
      661 ggtgaacaac agccacgaca ccagcagcga cacaaatatc gaaatgcccc gaaatagcca
      721 gtcgaacacc ggataatcgg gtatccctag ccatttcagc accgcagcca tcggcgcggg
      781 ctggttcagc gcggtgagca ccaaggtggc caggatcatc aggaacgtgc ccgccaacgc
      841 gatgaaatcg gatatcttgt tgcgcacgaa gcccggcgag tcggggggct gcacccacca
      901 catcccggtc accgcggcac gcagatgcga tatccatccc agtcccgccc aggcggcggt
      961 ggccaggccg atgacaccga ccgacgtccg cgcgtcgatc gccgagttca tcaggtcgag
     1021 gatctgctgg ctcaacgggc ccgacaccgt ggaccggacg tggtcgtcga tcgtccgcag
     1081 cagcgtcggc cgccgggcca gcatgaaccc gcacaccgcg aaaccgacca tcagcacggg
     1141 aaataacgcg aagatcgtgt agtacgtgag gccggccgca aagaagctgc cgttgcctcc
     1201 gtcgaaacgc tcataagcgc gcatggcatg gtcgaaccaa tcgaaccggg cccgtagccg
     1261 atcaaggatt ccgggcttgg ccggctcgct cattgtgcaa cctatttccg ccgcggaagg
     1321 aaccccaacc gatcgtagac ccgctcaacc gttttgccgg ccacatcgtc ggctcgctcc
     1381 gcaccggccg cgagcaccgc ctccaattgt gtgggatcgg ccagcaattc gtcgactcga
     1441 gccttgatgg gggtgacgaa ttcgacgacg gcgtcggcgg tgtccttctt caggtcgccg
     1501 tagccgcgcc cggcgtaacc ctcgacgagt ttgtcgatgc ccgccccggt gaccgccgac
     1561 tgaatggtca acaggttgga cacgcccggt ttggcatcgg tgtcgaaccg gatctcacgt
     1621 tcgctgtccg tcacggccga gcgaatcttc ttggcggaca acgccggatc gtcgagcaga
     1681 ttgatcaacc cggcatcggt ggacgccgat ttgctcatct tcgacgtcgg gtccgccagg
     1741 tcgtagatct tggcggtggc cttgggaatg agcacatcgg gcaccacgaa cgtgtccggg
     1801 aagcggccgt tgaaccgctg cgccacgtca cgcgccagct cgagatgctg gcgctgatcc
     1861 tcacccacgg gaaccaggtt gctgtcgtag gccagcacgt cggccgcctg caacaccggg
     1921 taggtgaaca aacccacggt ggtggagtcg ctgccctgcc gcgtcgactt gtccttgaac
     1981 tgcgtcatgc gggaggcctg cccgaagccg gtgaagcaac ccagcaccca cgccagctgg
     2041 gtgtgggcgg gcacgtggct ttgcacgaag acggtgctgc ggccgggatc gatacccagc
     2101 gccaggtact gggcggcggt ggccagggtc cggcggcgca gcgcctcggg atcttgcggg
     2161 atggtgatgg cgtgcaggtc gacgacgcag aagaaggcgt cccagtcatc tccccagtcg
     2221 tcttgcagtg cgacccactg ggtgacggcg cccaacgcgt taccaagatg aagggaatcc
     2281 gaagtgggct gcacgccgga gaagatccgg cgggatccgg taggggtgct catgatgccc
     2341 cgatcctttc acgcggaagg cacgcgattc gacgaccggt caccccttgg ttgcggtacc
     2401 ggcggtaccg catttaatgt cggcactatg cgcagtctgc tcggttcctt actcgtgagc
     2461 tccgctcact cgctgtcgcc gggggtgacg gctcgtccac cgatccacct cggctccgga
     2521 gaaccggtcg tgctgctaca tccgttcctg ctgtcccaga cggtatggga ggacgtggct
     2581 cagcagttgg cggacacggg ccgctacgag gtcttcgccc cgacgatggc cggccacaac
     2641 ggcgggccgt ccgccgggac ctggctcctg tcgtccgcgg cgctcgccga tcacgtggag
     2701 cgccagatgg atgaactcgg ctggcagacc gcccacatcg tgggcaactc gctgggcggc
     2761 tgggtcgcct tcgagctgga gcgacgcggg cgcgcgcgca ccgtcaccgg catcgccccg
     2821 gccggcggct ggacgcgctg gagcccggcc aagttcgagg tgatcgccaa gttcatcgtg
     2881 gtcataccgt tgctggcgct cgcccggttg ttcggaccgc gcgtgctgca cctgccgttc
     2941 agccggcggt gcgccacctt cgcgatcagc gcgtcgccgg atggcgtcag cgagcgtcaa
     3001 ctcagcagca tcgtcgacga cgtcgggcac tgtcccgcct acttccagct gctgctcaag
     3061 gcgctgctga tgcacggcct gcgggagctg gcgctcaacg ccgtcccggc gcacctcgtg
     3121 atttgcggaa aggaccggat tgtccccgca cccaggttca gccggcattt caccacttgt
     3181 ctgccggatg aaaaccacca ggtcaccacg ctcgaggggg tgggccacgt gccgatgttc
     3241 gaggcgccgg accgcatcac cgaggtgatc accggcttca tcgaacaaag ctgcccgaca
     3301 gactcgggcc gccggcaacc cgccggctag ctggccctct agttcgccag ggccttctct
     3361 aggttctctg cgatggaatt gaggaattcc tcgctgttct gccactcctg tttggggccg
     3421 atgaggatgg cgaggtcctt ggtcatcttc ccgtcctcga cggtggcgat gacgacctct
     3481 tccagctttt gcgcgaattc caccaccgcg ggcgtcttgt ccagtttgcc gcggtgcgcc
     3541 agcccacgcg tccacgcgaa gatcgacgca atcgggttcg tcgacgtcgg cttgcccgcc
     3601 tgatactgcc ggtaatggcg ggtgacggtg ccgtgcgcgg cttcggcctc gacggtcttg
     3661 ccgtcggccg tcatcaacac cgacgtcatc agccccagcg agccgtagcc ctgcgcgacg
     3721 gtgtcggact gcacgtcgcc gtcgtagttc ttgcacgccc acacgtagcc gccctcccat
     3781 ttgaggcacg cggcgaccat gtcgtcgatc aggcggtgct cgtaggtgag tccttcggcc
     3841 tcgaacttgt ccttgaactc ctcgtcgtag atgcgctgga actcgtcttt gaacatgccg
     3901 tcgtaggcct tgaggatggt gttcttggtc gacaggtaca ccggccatct ggcgttcagc
     3961 ccgtaggaga atgacgcgcg cgcaaaatcg cggatggagt ccttgaagtt gtacatcccc
     4021 atcacgacgc cgccgtccgg gggaatcgac accacctcgt gcaccatcgg ttcgctgccg
     4081 tcggcaggcg tgaaagtcag tgtgacggtg cccggttggt cgactttgaa attcgtcgcc
     4141 cggtattggt caccaaaagc gtgacggccg atgacgatcg gcttggtcca gcccggaacc
     4201 agccgcggca cattggagat cacgataggc tcgcggaaaa tcgtcccgcc caggatgttt
     4261 cggatggtcc cgttgggcga cagccacatc ttcttcaggt tgaattcctg gacgcgggcc
     4321 tcgtcggggg tgatggtcgc gcacttgaca ccgacgccgt gcttcttgat ggcataggcc
     4381 gcgtcgatcg tcacctggtc gtcggtgcgg tcgcggtgtt cgatgcccag gtcgtagtag
     4441 tccaaattga tgtcgagata cggcaggatc agcatgtcct tgatgagctt ccagatgacg
     4501 cgagtcatct cgtcaccgtc gagctctact acccggcctt tgaccttgat cgtggcttca
     4561 ttggacatcg gtgtccgaat cctccggcgt gggaagcatt ggcgagcggt tgctcgttag
     4621 gccagcctac aagcagcgtt cacccacctc agagagattg agctgtccca ttggcatcgg
     4681 ctcggtgaca tgcgataggc tttacgtcgg tcatgagcgc cagcatcaag ccccggcttg
     4741 ctggccggca accctccaac cgcggtgggg tgccccgggt gatgaccagg ttgagtagcc
     4801 aacagccggc tacgcggcaa gcgcgggtcc gccatgaccg ggcccctgag tagacaggga
     4861 aacgtgatgc gcatccaaac agctcattcg cgtctcattg tgtgccggtg ctagccgccg
     4921 gtcaccggtt ggcgtcatcg gcgatggcgt cccggtcgac cgcgagcgcc ccgatatccg
     4981 agtgaccccc tagcagaaag caccgcacgt gagttccgag agcagcaccg acagcaattc
     5041 cgagaccgac ccgaccgcgc attggtcatt cgaaaccaag cagattcacg ccggtcagca
     5101 gccggacgcg gcgaccaacg cccgcgccct gccgatctac cagaccacgt cctacacctt
     5161 cgacgacacc gcccatgccg ccgcactgtt cgggctcgag gtgccgggca acatctacac
     5221 ccggatcggc aatccgacca ccgacgtcgt ggagcagcgg atcgccgcgc tcgaaggcgg
     5281 tgtggccgca ctgttcttga gctccgggca ggcggctgag acattcgcca ttctgaacct
     5341 cgcgggtgcc ggggatcaca tcgtctccag cccgcggctt tacggcggca cttataacct
     5401 gttccactat tcgctggcca agctcggcat cgacgtcagc ttcgtcgacg atcccgacga
     5461 cctggactca tggcaggccg cggtccgacc gaacaccaag gcgttcttcg gcgagaccat
     5521 ttccaacccg cagatcgacg tgctggacac gcccggggtc gccgcagtcg cccacgcgaa
     5581 cggtgtgccg ctgatcgtcg acaacaccgt cgccacgccc tacctgatcc agccgttcac
     5641 ccagggcgcg gacatcgtgg tgcactcggc caccaagtac ctgggcgggc acggcgccgc
     5701 gatcgccggc gtcatcgtcg acggcggcac cttcgactgg acgcagggcc gcttccccgg
     5761 attcaccacg cccgacccca gttaccacgg cgtggtgttc gccgagctgg gaccgcccgc
     5821 gtttgcgctg aaggcccggg tgcagttgct gcgcgacctc ggatcggcgg cttcgccgtt
     5881 caacgcgttc ctggtggccc agggcctgga aaccctgagc ctgcggatcg agcgtcacgt
     5941 cgctaacgcg cagcgggtgg ccgagttctt ggaggcccac gacagcgtgc tgtcggtcaa
     6001 ctatgcggga ctgcccagct caccttggta cgagcgggca aaacggttgg cgcccaaggg
     6061 aaccggagct gtcctgtcgt tcgaattgga cggcggcatc gaagccggca aggccttcgt
     6121 gaacgcgctg cggctgcata gccacgtcgc caacatcggc gacgtgcgct cgctggtgat
     6181 tcacccggcg tcgaccaccc atgcacagct gagccccgcc gagcagctga ccaccggcgt
     6241 cagccccggc ctggtgcggc tggccgtcgg catcgaaggc atcgacgaca tcctggccga
     6301 tctggagctc ggcttcgccg cggcccgcaa atacggtgcg ccggtcagcg aaagcggcga
     6361 cccacgggcc gtgggcgcct tctgaggggc ttctgacatg acgatctccg acgtgccgac
     6421 ccagacgctg cccgccgaag gcgaagtcgg cctggttcac atcggctcgc tgaccaccga
     6481 gaacggtgag gtgatcgacg acgtctgtat cgcggtacag cgttggggtg agttgtcgcc
     6541 aacgcgcgac aacgtcgtgg tggtgctgca tgcgctcacc ggtgattcgc acatcaccgg
     6601 gcccgccgga cccgggcatc ccacacccgg ctggtgggac ggggtggccg ggcccggtgc
     6661 gccgatcgac accaaccgct ggtgcgcggt ggccaccaac gtgctgggcg gctgccgcgg
     6721 atccaccggg cccagctcgc ttgcccgcga tggaaagcct tggggctcaa ggtttccgct
     6781 ggtttcggtg cgcgaccagg tggaggcgga catcgccgcg ctggccgcgt tgggcatcga
     6841 tcaggttgcc gccgtcgtcg gcggatccat gggcggcgca agggcactgg aatggattgt
     6901 cggccacccc gaccgcgtcc gggccgggct gctgctggcg gtcggcgcac gcgccactgc
     6961 cgaccagatc ggcacccaga ccacgcaaat cgcggcgatc aaggccgacc cgaactggca
     7021 gggcggcgac tactacgaga cggggaggtc accggacgcc gggatggcga tcgcccgccg
     7081 cttcgcccac ctgacctacc gcggtgaagt cgagctcgat acccgcttcg ccaacgacaa
     7141 ccagggcgac gagaacctgg cgaacggcgg ccgatattcg gtgcagagct acctcgagca
     7201 ccagggcgac aagctgttgg cccgctttga cgccggcagc tatgtcattt tgaccgaggc
     7261 gctgaacagc cacgacgtcg gccgcggccg cggcggggta ggtaacgccc tgcgcaggtg
     7321 cccggttccg gtcgtggtcg gtggcatcac ctccgaccgt ctctatccgt tgcgtttgca
     7381 ggaggagctg gcggagctgc tgcccggctg cgccggcctg catgttgtcg agtccatctg
     7441 cgggcacgac ggcttcctgg tggagtccga cgccgtcggc gacttgatcc gcgaaacgct
     7501 cgacttggcc ctcgacacag acactgccga aggcgcgtgt ctcaggtgag caaggtgacg
     7561 cgctccaggc acgaccgctc cctctcattc ggtccagcgg ctgctgccta cgagcgtggc
     7621 cgcccctcct acccgccgga ggcgatcgac tggctgttgc cgcgcggtgc gcgccaggtg
     7681 ctcgacctgg gtgcgggcac cggcaagctg accacccggc tggtcgagcg cggcctggaa
     7741 gtggtggccg tcgaccccat tcccgacatg ctggaagtgc tgcgagtctc actgccggaa
     7801 acccgcgcgc tgctgggcac cgcggaagag attccgttgg aagacaacag cgttgacgct
     7861 gtgctggtcg cacaggcgtg gcactgggtg gatcccgagc gggcgattcc cgaggtggcc
     7921 cgggtgctgc ggccgggagg gcggctgggc ctggtgtgga acactcgcga cgaacggctg
     7981 ggctgggtgc gcgagctggg ccagatcatc ggttccgatg gcgaccgggg ccgtttcgac
     8041 ctcacgctgt ccacagcgtt caccgagccg gagcgccatc aagtcgagtg gacgaattac
     8101 ctgacgcccc aagcccttat cgacctggtg gcgtctcgca gctactgcat cacgtcgccg
     8161 accgaagtcc gctccaggac actcgaccag gtgcgtgagc tgctggccac ccacccggcg
     8221 ctggcgaatt cgacgggcct ggcgctgccg tatgtcaccg tatgcatcag ggcgacggtg
     8281 gccgagtaac ttgcgagtgt cctgaaacgc aacgggattc agtcggtggc ttgcactcgg
     8341 gaccgaagga gttgcacacc aggaccgttg agctcgtcga ggtagggcgg gcgaccggcc
     8401 atgctcatta ccagggccag ggttgatccg ctgaccaggg caccgcaacc cgcgctaaaa
     8461 ggcccgtcgt ctgcccgcaa gcagaggccg gcgatgcggg tacgactggc aacggtgaag
     8521 tcgcgtcggg cgaagaatgc ggccacaggt gtcagcgcgt cgacgctcgg tgtccgcacc
     8581 agccccagag gctgacgaat gtcttgggca tgcacaacga cctcaccgag gtaggccggg
     8641 atgtgggctg agggcgcggt ggtgctattg atgacggcgc gaaaccggtc cagtgtctcg
     8701 gcgggggtgc taccacggtg ctcgatcagc cgacgctggt tgtgcacgtc gggacgcaaa
     8761 cgcgcgccga gcatgctgcg cagccacttc cactgattca ggctcgccgc ggcggtcagg
     8821 tgcgcgacga cctcctcaac gtcccatttc ccgcacaagg tgccgtgccg ccactgctct
     8881 gcgctgaggc ccgagagatc ctccgccagc gccgcgcgct cggcgtgcgc cagtgcccac
     8941 aggtgatcgt cctggggttt cgccatatcg ctccactccg tgacattggc cttcacattg
     9001 gtgccgtcac tcgaggtaga cccgcgccgg ccgcaaaatt catcgaaccg cggctggaag
     9061 gcctggacct gggcacgggc tcgaccggcc cggccgagcg cgccggtcaa ccgattgaaa
     9121 cccgggcttc gtccgatcag atcaacgcgg cagtgcgcac attcccatac gtcctggcga
     9181 cccttttacc ggcatcggac aaattgattt tcgcaataca ttccgacacc accccagccg
     9241 cgtgacggac cgccttcgtt ggtcggattc acagacagtg ccggagtctg ggtcagtatt
     9301 tgatcgatta tcccgggccc cgccggcgta aaggggtctg aatccgcgtg tgctatgccg
     9361 gtgccgcccg cgagggccat gacgctcatc gctacggcca gtagtccgcc agcgcacgtc
     9421 tgaggtttca tcgccaggcc cctctttcgt ctaccccgga tcactgatta tcgaccagca
     9481 acaattctag cggtctggca aacgaagtga gcacaggatt tttggtccac gccgacgagc
     9541 gccaccggtc cccccagcat ttcgcaccca tatcgcataa cggttacgaa cgcacaacga
     9601 catcggccgg tcgaccagca aatttcgcaa cctgaatcgg accgtgcgta cggtgtcgcc
     9661 cgtgaggccg atagccgtgg tcgttcgtcg cgctgcgatt ctcgccgtct gcctcgcaat
     9721 gtcactgacc gtagcgcctc caaccgccgc tgatcctgac accgagcctg ccgccgctgc
     9781 ggcggaggca ccggacgacg ggcgggtgcc ctcggccgat caggtaacct tcaacaccac
     9841 cgacggatgg accctggccc tcggggcgaa ggacgagtcg caagttcccc tggcgccgtt
     9901 gaccaccgcg gtctcgtctc gcgaatacct ctcccgcgga acattcttcg cttcgctgga
     9961 aggaccggaa cagcctcgcg gcgtcctcga ggtgggttac gagatcggct gcggaatcga
    10021 tatgagcacg tccaacggcg tcacgatggc gggtagcgcc ggtctcaacc cgggcctcgg
    10081 ctttccgttg gccggtggtt tta
//
LOCUS       DASSGQ010000315         3972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_509833,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000315 DASSGQ010000000
VERSION     DASSGQ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509833"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..510
                     /locus_tag="VFI55_13865"
     CDS             322..510
                     /locus_tag="VFI55_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075153.1"
                     /translation="MRAWAMLQQWSDSWCSRLGIVVDQAFGLLWCRMIFAHQSLDKRA
                     ADDLAAALATQLGANQEG"
     gene            513..911
                     /locus_tag="VFI55_13870"
     CDS             513..911
                     /locus_tag="VFI55_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7075154.1"
                     /translation="MGFAVERFDHIVVNCNDVEATAAWYEGVLGMTRETFGPSQRTAL
                     SFGNQKINLRPVGALADDAAWVTGSVEAAGSEDLCFITRATPEEVRAHLVACGVELTS
                     GPVTKIGALGPMTSHYCRDIDGNLIEIAVY"
     gene            complement(917..1372)
                     /locus_tag="VFI55_13875"
     CDS             complement(917..1372)
                     /locus_tag="VFI55_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7075155.1"
                     /translation="MGSSDGFHPDFDDTATEITRNRVHHVKSSDISSDTAQSEGMRRF
                     AALSGRSVGAEKLWMGETHVSPRTASSNHHHGESETAIYVRSGNPEFVFHDGVQEVRI
                     STAPGDYIFVPPYLPHREENPDPNTPAEVVIARSTQEAIVVNLPELYPL"
     gene            complement(1384..2841)
                     /locus_tag="VFI55_13880"
     CDS             complement(1384..2841)
                     /locus_tag="VFI55_13880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7075156.1"
                     /translation="MAELERDYDVVVLGAGPIGQNAAERARAAGLRVAMVERELVGGE
                     CSYWACVPSKALLRPVIAVSDARRVDGAREAVTGAISTAGVFGRRNRYVSDWDDSGQA
                     EWVRGIGATLVRGHGRLDGPRRVAVSTPGGEQPVLTARHAVVVCTGSRPALPELPGIA
                     EARPWTNREATDSSYVPTRLAVVGAGGVGVEMATAWQGLGSKVTLLARGSGLLPRMEP
                     FVGELIGRGLAAAGVDVRVGVSVRALHRSDSGPVALELDDGSELEVSEVLFATGRAPL
                     TDDIGLATVGLTPGSWLDVDDTCRVRAVDDGWLYAAGDVNHRALLTHQGKYQGRIAGA
                     AIGARAAGIPLDTTPWGAHATTADHYAVPQAFFTDPEAAAVGLTALQAEQAGHRVHTV
                     DVEIGDVVTGAKLYADGYSGRARMVVDADRDRLLGVTLVGPGVTELLHAATIAVAGQV
                     PIGRLWHAVPCFPTISELWLRLLEAYRDSSTDDTH"
     gene            complement(2951..3655)
                     /locus_tag="VFI55_13885"
     CDS             complement(2951..3655)
                     /locus_tag="VFI55_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HET7075157.1"
                     /translation="MNTHPRPPAPSVLVFDVNETLIDIDSIAPLFGELFGDERVLREW
                     FGQLVMYSMTATLAECYVDFFTLGQGVLHMLADIHRVDITDDDIRRLRAQMSTMPAHL
                     DAVGGLTTLRENGFRLVTLTNSPHRPGTRTPLENAGLAELFDRQFSVESCRAFKPAPS
                     VYRYVCHELGVLPADCMMVAAHVWDTLGAQNVGFSTALITRPGNPPLPVEALPQPDIV
                     VGDLHQLAERLIDGGR"
     gene            complement(3786..>3972)
                     /locus_tag="VFI55_13890"
     CDS             complement(3786..>3972)
                     /locus_tag="VFI55_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7075158.1"
                     /translation="FVFGNPGFPLSDVPLQDIARQVAEGKLDAKPSRVFSFDQIHEAH
                     RVMDAGEATGKMVVVMS"
BASE COUNT          670 a         1279 c         1306 g          717 t
ORIGIN      
        1 cggtaactcg aattgcatga cgatacgcag ctcgcggtga tggagaccgc ccgaactgca
       61 tggcaccttt atgacgatgt ggcttgctgt tcgatagtgg caaccctacg cctcgcccac
      121 acgattgtga cgaaaagatc ccggtagata tatcgagcac tggataaatt gcgtcgacca
      181 ccagatatcg agcaattgaa tactggagaa tgcatcaggt gtattttcgc tcggcaatct
      241 ggcatctgcc gttacgtagg ttggcgtccg tgcgtgcccg gcactgaggt gtgatgaggt
      301 gcccgttggg ggtgcgacgg tgtgagggcc tgggcgatgc tgcaacaatg gagcgactca
      361 tggtgttcgc ggctgggcat cgtcgtcgac caggcattcg ggttactgtg gtgtcgaatg
      421 attttcgcgc accagtcgct cgacaaacgc gccgccgacg atctcgccgc cgcgctcgcg
      481 actcagctcg gcgcgaatca ggagggatag aagtgggatt cgcggtagag cggttcgacc
      541 acatcgtcgt gaattgcaat gacgtggagg ccaccgcggc gtggtatgag ggggtgctgg
      601 gcatgacgcg agagacgttt gggccgtccc agcggaccgc cctgtccttc gggaatcaga
      661 agatcaacct acggccggtc ggggcgttgg ccgacgacgc cgcctgggtc accggttcgg
      721 tcgaggcggc cggctccgaa gacctgtgct tcatcacccg tgccaccccg gaagaggtgc
      781 gcgcccacct cgtcgcctgc ggggtcgagt tgacctccgg gccggtgacc aagatcggtg
      841 cgctgggccc gatgacgtcg cactactgcc gggacatcga cggtaatctc atcgagatcg
      901 ccgtgtactg aacgcgttag agcgggtaca gctcgggcag gttgaccacg atggcttctt
      961 gtgtgctgcg cgcgatcacc acttcggcgg gtgtgttcgg gtcggggttt tcctcacggt
     1021 gcgggaggta cggcggcacg aagatgtagt cgccgggcgc agtcgagatg cgcacctcct
     1081 ggacgccgtc gtgaaacacg aactccgggt tgccacttcg cacatagatc gccgtctccg
     1141 actcgccatg atggtggttg gaagacgcgg ttctgggcga cacgtgcgtc tcgcccatcc
     1201 acagcttctc cgcgccgacc gaccggccgc tgagggccgc gaagcgccgc atcccctccg
     1261 attgggcggt gtcggagctg atgtccgacg acttgacgtg gtggacgcgg ttgcgcgtga
     1321 tttccgttgc ggtgtcatcg aagtcggggt ggaatccgtc cgagctgccc atgagcgatc
     1381 ctcctagtgg gtgtcgtccg tggatgaatc ccgataggcc tccagcagtc tcagccacag
     1441 ttcgctgatg gtcgggaagc atggcacggc gtgccataac cgaccgattg ggacttggcc
     1501 tgccacggcg atggtggcgg catgcagcag ctcggtgaca cccgggccga ccagagtgac
     1561 gccgagcagg cggtcccgat ccgcatcgac caccatcctc gcccgaccgg agtacccgtc
     1621 cgcatagagc ttggctcccg tcaccacgtc gccgatttcg acgtcgaccg tgtgcactcg
     1681 gtgtccggct tgctcagcct gcaaagccgt gagcccgacg gcggcggcct cgggatcggt
     1741 gaagaacgcc tgtggcacgg cgtaatggtc ggcagtggtc gcatgggcgc cccacggcgt
     1801 ggtgtccaac gggattcccg cggcgcgggc tccgatggcc gcaccggcga tacgtccctg
     1861 gtatttgccc tggtgggtca gcagggcccg atgattgacg tcgcccgccg cgtagagcca
     1921 accatcgtcg acggcccgta cccggcaggt gtcatcgacg tcgagccagc tgcccggcgt
     1981 cagtcccacc gtcgccaggc cgatgtcgtc ggtgagcggt gcccggcccg tggcgaaaag
     2041 cacctcgctg acttccaact cggatccgtc gtccagttcg agggccaccg ggccgctgtc
     2101 ggaccgatgc aatgcgcgaa ccgatacccc gacgcggacg tcgacgcccg cggcggcgag
     2161 tccgcggccg atgagttcgc cgacaaaggg ttccatccgg ggcaacaggc cggatccgcg
     2221 cgccagcagg gtcaccttgg agcccagtcc ttgccaggcg gttgccattt cgactcccac
     2281 gcccccggcg ccgacaacgg cgagccgcgt cggcacgtag ctgctgtctg ttgcctcgcg
     2341 gttggtccac ggcctcgcct cggcgatgcc gggcaactcg ggaagtgcgg gccggcttcc
     2401 ggtgcacacc acaaccgcgt gccgtgcggt cagtaccggc tgttcgccgc cgggcgtgct
     2461 gacggcgact cgtcgcggac cgtccaaccg cccgtggccg cgcaccagcg tggcgccgat
     2521 tccgcgcacc cactcggcct ggccgctgtc gtcccagtcg ctgacatagc ggttgcggcg
     2581 gccgaagaca ccggcggtgc tgatggcacc ggtgaccgct tcccgcgcgc cgtcgacccg
     2641 acgcgcatcc gacacggcga ttaccggccg aagcaacgcc ttactgggca cacacgccca
     2701 gtacgaacat tcgcctccga cgagttcgcg ctcgaccatt gcgacgcgca ggcccgcggc
     2761 ccgggcgcgc tccgcggcgt tctgcccgat tggtccggcg ccgagcacca cgacgtcgta
     2821 gtcccgttca agctctgcca tgatcgactg tcctccctcg cgccgagcgt gaactcacgg
     2881 cgaaattctc gccgattttc cgccctgacg tcacgctcgg cgaaagtccg cgacaagtaa
     2941 cggcaaatat tcagcgcccc ccatcgatca gccgctcggc cagctggtgc aagtcaccca
     3001 ccacgatgtc gggttgcggc agagcctcga ccggcagcgg tggattgccg ggccgcgtga
     3061 tgagggcggt gctgaaaccg acattctgcg cgccgagggt atcccacacg tgcgcggcca
     3121 ccatcatgca gtccgcgggc agtacgccta actcgtggca cacataccgg tagaccgacg
     3181 gagccggctt gaaggcgcgg caggattcga cgctgaactg ccggtcgaac agttccgcga
     3241 gcccggcgtt ctcgagtggc gtccgcgtcc caggtcgatg cggcgaattc gtcagcgtga
     3301 ccagacgaaa gccattctcg cgcaatgtcg taagtcctcc gacggcgtcg aggtgcgccg
     3361 gcatggtgct catctgcgcc cgtaggcgac ggatgtcgtc gtcggtgatg tccacccggt
     3421 ggatgtcggc aagcatgtgc agcacgcctt gaccgagggt gaaaaagtcg acatagcatt
     3481 cggccaaggt cgcggtcatc gagtacatga cgagctggcc gaaccattcg cgcagcaccc
     3541 gttcgtcacc gaagagctcg ccaaaaagtg gcgcgatgga atcgatgtcg atgagcgtct
     3601 cgttcacatc gaagacgagt acagacggtg ccgggggcct ggggtgtgtg ttcatgccgg
     3661 tcgttcgtcg ggtctggccg ggcccgacct ctggccgtct gccgtcacga taaccattgt
     3721 catatcctcg ctctccgaaa ccggttgcca gagtgcctat ttgcggtcgt ttaacactgt
     3781 gcggctcacg acatcacgac gaccatcttg ccggtcgctt ctccggcgtc catcactctg
     3841 tgtgcttcat gaatctggtc gaaggagaag acgcgggacg gctttgcatc cagcttgcct
     3901 tcggcgacct gccgggcgat gtcctgtagc ggcacgtcgg agagcgggaa gccgggattc
     3961 ccaaacacga ag
//
LOCUS       DASSGQ010000316        11724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_174932,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000316 DASSGQ010000000
VERSION     DASSGQ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11724
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174932"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..505
                     /locus_tag="VFI55_13895"
     CDS             71..505
                     /locus_tag="VFI55_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET7075159.1"
                     /translation="MTQQTPDVDMLNAFNKNIIDEFRANDGKVTGQFANANLLLLTTT
                     GAKSGQQRVSPLAYFRVDGKLIIVGSFAGAPVNPAWVHNLRANPRAHVEVGTDALDVT
                     ARELAPAERDALFDKVAAAAPGFAEYQSKTSRVIPLFELQPA"
     gene            558..1625
                     /locus_tag="VFI55_13900"
     CDS             558..1625
                     /locus_tag="VFI55_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016719422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HET7075160.1"
                     /translation="MTTSLSAVQSAAYAAGIAGVQRLRGRAFNLLQTAVASGLAWYIA
                     HDVLGHPQPFFAPIASAVCLSISNVLRAQRAIQMIIGVTSGIGIGTVVLALLGPGALP
                     IAVATLIALSLAVLIGQGFIGQGMMFANQTVVSSILVLALPRSGVGWERIYDALIGGC
                     LAIVFAVLLFPADPLRVLRRARVGVLRVLQSVLTGTGELAAGRRVPPPDWPLSAVDRV
                     HGQLGGLMQARATARQAVRISPRRWNMRDSVYAADHQAIHVALLAGSVLELARAVAPT
                     VDGCCHRLPHPVQAVLVDLATATALAESDPAGAAAYTAAARRHASQLESDARERNEVV
                     LADVARACVDDLQRVIDLRQP"
     gene            complement(1640..2029)
                     /locus_tag="VFI55_13905"
     CDS             complement(1640..2029)
                     /locus_tag="VFI55_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET7075161.1"
                     /translation="MATWNDVARVVGELALTSEPAPHDWRVGKKLLAWERPLRLSDRE
                     ALAQRGVEPPEGDILGVRVSDEGVKFALIADEPKVYFTTPHFDGYPAVLIRLAEIEVP
                     DLAELITEAWMTQAPRKLVQEFLAGSL"
     gene            2168..3118
                     /locus_tag="VFI55_13910"
     CDS             2168..3118
                     /locus_tag="VFI55_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009955932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HET7075162.1"
                     /translation="MSEGVSAAQAPAARRHRVTHRTKYRYSDTVTSSYGRGFLTPRDS
                     LRQQCIAHRLIVDPAPADSSTSQDIYGNISSYFHVTQPHRILTVTSDSIVDVYPPAPG
                     LHTSGPAIEPWEAARPTGRRGALAAEFALDLNPPEITDEVRDYAAPSFVPERPLIDVL
                     RDLASRIFTDFTYRSGSTTVSTGVNEVLLAREGVCQDFARLAIACLRANGLAASYVSG
                     YLATDPPPGKDRMFGIDATHAWAAVWTPQESGQFAWLGLDPTNDQMVDQRYIIVGRGR
                     DYADVPPLRGIIYTNSEHSVIDVAVDVVPYSDAEGDFLHA"
     gene            3111..4136
                     /locus_tag="VFI55_13915"
     CDS             3111..4136
                     /locus_tag="VFI55_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HET7075163.1"
                     /translation="MRDFHCPKCGQRLVFENSACLSCGSALGFSLDQMALLVIAKGDA
                     GDHPGAVDADEYQLCANLHLAECNWIIPRNDPGGLCASCVLTLERPHDADTEGLAAFA
                     RAEAAKRRLIAELHELKLPILGRDPDPDYGLGFRLLSSAHEEVLTGHDNGVITLDLAE
                     GDDVHREQLRVEMDEPYRTLLGHFRHEVGHYYFYRLISPSSEYLAGFNELFGDPYADY
                     SAALDRHYRDGAPDGWQKSFVSSYATMHPAEDWAETFAHYLHIRDTLDTSAWCGLAPA
                     SATFDRPALGPSAFPTIIEMWLPLSWSLNMVSRSMGRDDMYPFVLPAAVLNKMQFIHT
                     VIDEVGG"
     gene            complement(4133..6808)
                     /locus_tag="VFI55_13920"
     CDS             complement(4133..6808)
                     /locus_tag="VFI55_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HET7075164.1"
                     /translation="MSEATAAPGIAGGAYDTDRLLAGYRTARAQEALFDLRHGPGTGY
                     DEFVDRQGNVRPAWGELADAVAERGRAGLDRLRAVVESLIDNDGITYTEVDSDRDEAV
                     GGHGVEPRPWSLDTLPIVVSAADWDMLETGLVQRSRLLDAVLTDLYGRRTLLTEGVLP
                     PELVFAHPGYVRAANGIEIPGHHQLFMHACDLSRLSDGSFQVNADWTQAPSGAGYALA
                     DRRVVAHSIPDLYERIAPRPTTPFAQMLRLALIDAAPDVAQDPVVVVLSPGIYSETAF
                     DQAYLATLLGFPLVESADLVVRDGKLWMRSLGTLKRVDVVLRRVDAEYTDPLDLRADS
                     RLGVVGLVEAQRRGTVTVVNTLGSGILESPGLLRFLPELAKRLLGEAPLLHSAPVYWG
                     GIASERSHLLANLSSLLIKSTVGENTLVGPTLSSAQLTELAAQIEHMPWHWVGQELPQ
                     FSSAPTDHGAVLSSAGVGMRLFTVAQRSGFAPMIGGVGYVLAPGPAAYTLKTVAAKDV
                     WVRPTARARAETVTVPSVEAAVAAPAKTAAGTWGVSSPRVLSDLFWMGRYSERAEGMA
                     RLLIVARDRFHVYRHHQDTEESECVPVLMAALGRITGTDTGVDGDQAEMIAVAPSTLW
                     SLTVDPSRPGSLVQSVDGLALAARAVRDQLSNDTWMVLAGVERAIGLDSEPPDSLAEA
                     DVLLASAQAQTLSGMLTLSGVAGESMVRDVGWTMMDIGKRIERALWLTALLRATLTDV
                     HSAAAEQAIIEAALVACESSVIYRRRTVGKVSVAAVAELMLFDANNPRSLLYQVERLR
                     ANLKDLPGSSGSSRPERLVDEINTLVRRSHPGELEAVSASPSGGARRAKLAELLDAIH
                     VELRDLAEVITATQLALPGGMQPLWGPDERRVMPA"
     gene            complement(6843..10193)
                     /locus_tag="VFI55_13925"
     CDS             complement(6843..10193)
                     /locus_tag="VFI55_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HET7075165.1"
                     /translation="MSIKVALEHRTSYVFDRLVRVYPHVVRLRPAPHSRTAIEAYSLR
                     VEPSEHFINWQQDAVGNFLARLVFPGPMNRLTITVGLIADLKVVNPFDFFIEDWAEIW
                     PAGGLIYPKEMADDLRPYLRPVDEDAEGSGPGELVQAWLRNFTVPEGTRTIDFLVSLN
                     RAINADVTYSLRMEPGVQTPDFTLRAGVGSCRDSAWLLVSILRQFGLAARFVSGYLVQ
                     LASDVEALDGPSGPAADFTDLHAWTEVYIPGAGWIGLDPTSGLFAGEGHIPLAATPHP
                     STAAPITGGTEPCESVLEFSNTVTRVHEDPRVTLPYTDQVWATICGVGRRVDERLNAA
                     DVRLTVGGEPTFVSVDNQTDEEWTTAADGPHKRQRASDLAMRLKSVWAPQGLIQRSQG
                     RWYPGEPLPRWQIALYWRTDGRPLWTDDTLLADPWATELVPPAPVDDGSAYQVLAGIA
                     DDLGLPLSQVRQAYEDPLSRLAAKVRTPAGDPVEPSDDLAPEAGSDAHSDSEAGRAAL
                     LARLDESTSAPAAFVLPLHRRDDELGWASANWRLRRGRIVLIEGDSPAGLRLPLSSIS
                     WQPPRASLDADPLTVGSELAAEPAASDAVVTDSDTAPPTALVAEVRGGLLYIFIPPTE
                     ALDHFVDLIGRVEAAAAKADCPVVIEGYGPPPDPRLTSTTITPDPGVIEVNVAPTASF
                     AEQTQLLETLYGQARLARLSTESFDIDGTHGGTGGGNHITLGGVTPADSPLLRRPDLL
                     VSLLTYWQRHPSLSYLFAGRFVGTTSQAPRVDEGGSDALYELEIAFAEIARLSAGGAP
                     KPWVTDRALRHLLTDITGNTHRAEFCIDKLYSPDSPRGRLGLLELRGFEMPPHLRMAM
                     VQSLLVRSLVAWFWDEPLRAPLIRHGANLHGRYLLPHFLIHDIADVAADLRAHGIPFE
                     TSWLDPFTEFRFPRIGTAVFDGVEIELRGAIEPWKTLGEEASATGTARYVDSSVERIQ
                     IRIIGADRHRYVVTVNGYPVPLLATDNPDIHVGGVRFKAWQPPSALHPTITTDVPLRF
                     ELIDLTSGTSRGGCTYHVAHPGGMAYDEPPVNAVAAESRRARRFEATGFTPGKLDLAV
                     IRERQARIFTDVGAPGILDLRRVRTVQR"
     gene            complement(10319..11407)
                     /locus_tag="VFI55_13930"
     CDS             complement(10319..11407)
                     /locus_tag="VFI55_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075166.1"
                     /translation="MSQPPEYPGSPPDPHRGNQNPPGYPPPPGYGAPPPPPPPSAPGY
                     GPPPGYAPPPGPPHGYGAPPPPPPPGPPGGYGAPPPGYGPPPGYGPGGPGGPGGFGGP
                     GGPGGPGGPPPAAFSVGDAFRWAWGKFTSNAAALVVPVLIYGLILGIVGGLTSFLPAA
                     LGEQSSTTYTDAYGQTSASTSVTLGTASIAVMVVGYILIFLVAVVMHAGLLSGALDLA
                     DGKPVTIGSFFKPRNLGGVILAALLVGIGTWIGTLLCVIPGIVFGFLAMFAIPFVVDR
                     SLSPIDSIKASFATTRSNVGGALLSWLVQIAAVLVGELLCLVGLLVGLPVALLVLTYT
                     YRKLSGGQVVALEQPGYPPAPPPGPYTG"
     gene            11548..>11724
                     /locus_tag="VFI55_13935"
     CDS             11548..>11724
                     /locus_tag="VFI55_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET7075167.1"
                     /translation="MPKPVSDGLFDVPGPPQTTNHDLGVSAGAPLAVRMRPASLDEVV
                     GQRHLLAPRSPLRRL"
BASE COUNT         1845 a         4008 c         4078 g         1793 t
ORIGIN      
        1 ccgcgccgat caccgcccag cagcgcaagg agtccggaat agacgccaag cccaaggggg
       61 ttcagcaggc atgacccaac aaacgccaga cgttgacatg ctcaacgcgt tcaacaagaa
      121 catcatcgac gagttccggg ccaacgacgg gaaggtgact ggccagttcg ccaacgccaa
      181 cctgttgctg ctcaccacga cgggcgccaa gtccggccag cagcgagtgt cgccactggc
      241 gtatttccgc gtcgacggca agctgatcat cgtcgggtcc ttcgccggcg ccccggtcaa
      301 cccggcctgg gtgcacaacc tgcgggccaa tccgcgcgcc cacgtcgagg tcgggaccga
      361 cgcgttggac gtgacggctc gcgagctggc gcccgcggag cgcgacgcgc tgttcgacaa
      421 ggtcgcggcc gcggcgcccg gcttcgccga gtatcagtcg aagaccagcc gggtgattcc
      481 gctgttcgag ttgcagccgg cgtaactcct tggggatacc gcgcgcgccg cgccgcccgc
      541 tgaggtaagc aagcctgatg acgacttcgc tgtcggcggt gcagtctgcg gcgtacgccg
      601 ccgggatcgc gggtgtgcaa cggctgcgtg gccgggcgtt caacctgctg cagaccgcgg
      661 tcgcctcggg tctggcctgg tatatcgcgc acgatgtgct cggtcatccg cagccgttct
      721 tcgcgcccat cgcctcggcg gtgtgcctgt cgatcagcaa tgtgttacgc gcgcaacgcg
      781 ccatccagat gatcatcggt gtgacgtcgg ggatcgggat aggcaccgtg gtgctggcgc
      841 tgctcggacc gggagccctg cccatcgccg tcgccacgct gatcgcgctg tcgctcgcgg
      901 tgctgatcgg gcagggcttc atcggccagg ggatgatgtt cgccaaccag accgtcgtgt
      961 cgtcgatcct ggtgctggcg ttgccccgca gcggcgtcgg ttgggaacgc atctacgacg
     1021 ctctcatcgg tggttgcctg gcgattgtgt tcgccgtcct gctgtttccg gccgatccgc
     1081 tcagggtgtt gcgcagggcg cgcgtcggcg tgctgcgcgt cctgcaatcg gtcctgaccg
     1141 gcacggggga actcgccgcc ggacgccggg tgccgccgcc ggactggccg ctgtcggccg
     1201 tcgatcgggt gcacgggcag ctgggcgggc tcatgcaggc ccgcgccacc gcgcgccagg
     1261 cggtgcggat ttcgccgcgg cggtggaaca tgcgcgattc cgtatacgcc gccgatcacc
     1321 aggcgataca cgtggccctg ctggccggct ccgtattgga gctggcccgt gccgtagcgc
     1381 ccaccgtcga tggctgctgc caccggctgc cgcacccggt gcaggccgtg ctggtcgacc
     1441 tggcgacggc aaccgccctc gccgagtccg atcccgccgg ggcggccgca tacaccgcgg
     1501 ccgcgcgccg ccatgcctcg cagctggaat ccgacgcccg cgagcgaaac gaggtcgtgc
     1561 tcgccgatgt cgcccgggcc tgcgtcgacg atctacagcg ggtgatagac cttcgccagc
     1621 cgtgaacgct gcggccggct cacaacgagc cggccaggaa ctcctgaacg agtttcctgg
     1681 gcgcctgcgt catccacgcc tcggtgatca actccgcgag gtcagggact tcgatctcgg
     1741 ccagcctgat cagtaccgcc ggatagccgt cgaaatgtgg ggtggtgaaa tacaccttcg
     1801 gctcgtcggc gatcaacgcg aacttgacgc cctcgtcgga cacccggacg ccgaggatgt
     1861 cgccctccgg cggctcgacg ccgcgctgcg ccagcgcttc gcggtccgac agccgcagtg
     1921 gccgctccca cgccagcaac ttcttgccga cccgccagtc gtgcggggcc ggctccgagg
     1981 tgagtgccag ctcaccgacg acgcgggcga cgtcgttcca cgtggccacg tctcgattgt
     2041 gctccggtta tggcgcacgc ggccccttat cggcaaacga tggggaactt ggaccggcgt
     2101 gaacccaccg gatcatcccg tttgtggtgt gatcagccgg tcggatcccg acgtgaggag
     2161 gtagctcttg tcagaaggtg tgtcagcagc gcaggccccc gccgcacgcc gccatcgggt
     2221 cacccaccgc accaagtacc gctactcgga caccgtgacc agctcgtatg gccgcggttt
     2281 cctcacgccg cgggactcgc tgcggcagca gtgcatcgct caccggctga tcgtcgaccc
     2341 ggcccccgcc gacagctcca ccagccagga catctacggc aacatcagct cctatttcca
     2401 cgtcacgcag ccgcaccgca tcctgacggt caccagcgac tccatcgtcg acgtgtatcc
     2461 accggctccc gggctgcaca ccagcgggcc ggcgatagaa ccgtgggagg ccgcccgccc
     2521 gaccgggcgc cggggagcgc tcgcggccga gttcgcgctg gacttgaatc caccggaaat
     2581 caccgatgag gtgcgcgact acgccgcccc cagcttcgtc ccggagcgcc cgctgatcga
     2641 cgtgttgcgc gatctcgcgt cgcggatttt caccgacttc acctaccgct cgggatccac
     2701 cacggtgtcc accggagtca atgaggttct gctggcccgg gaaggggtat gccaggactt
     2761 cgcgaggctg gcgatcgcct gcctgcgagc caacggcttg gcggccagct acgtgtccgg
     2821 ttatctggcc accgacccgc caccgggaaa ggatcgaatg ttcggtatcg acgccaccca
     2881 tgcctgggcg gcggtgtgga ccccgcagga gtccggccag ttcgcatggc tcgggttgga
     2941 ccccaccaat gaccagatgg tcgaccagcg ctacatcatc gtgggccgcg ggcgcgacta
     3001 tgcagacgtg ccgccgctgc gcggcatcat ctacaccaac tccgaacaca gcgtgatcga
     3061 tgtcgccgtc gacgtggtgc cttactcaga cgccgaaggc gacttcttgc atgcgtgatt
     3121 tccactgccc caagtgcggg cagcgcctgg tgttcgagaa ttcggcgtgc ctgtcctgcg
     3181 gtagcgcgct ggggttttcc ctcgatcaga tggccctgct ggtgatcgcc aagggcgacg
     3241 cgggtgacca tcccggtgcg gtggacgccg acgagtatca actgtgcgcc aatctccatc
     3301 tcgcggaatg caattggatc atccccagga atgaccccgg cgggctgtgt gcatcgtgcg
     3361 tgctcacgct ggaacggccc cacgacgccg acaccgaggg cctggccgcc ttcgcccgcg
     3421 ccgaggcggc caagcggcgg ctgatcgccg agctgcacga gctgaagttg ccgatcctgg
     3481 ggcgcgaccc cgatccggac tacgggctgg gcttccgcct gctgtccagc gcccacgagg
     3541 aagtactgac cggccacgac aatggcgtca tcacgctgga cctggccgag ggcgacgacg
     3601 tccaccgcga gcagctacgc gtcgagatgg acgagccgta ccggaccctg ctggggcact
     3661 tccggcacga ggtcggccac tactacttct accggctgat cagcccgtcg agcgagtacc
     3721 tggccggatt caacgagctg ttcggcgatc cctacgccga ctactccgcg gcgctggacc
     3781 gccattaccg cgacggcgcc ccggacggtt ggcagaagag cttcgtgtcg tcgtatgcga
     3841 ccatgcaccc cgccgaggat tgggccgaga cgttcgccca ttacctgcac atccgcgaca
     3901 cgctggacac ctcggcctgg tgtggcctgg cgccggcgtc ggccacgttc gaccgtccgg
     3961 cgttggggcc cagcgctttt cccaccatca tcgagatgtg gctgccgttg tcgtggtcgc
     4021 tgaacatggt gagccggtcg atgggccgcg acgacatgta ccccttcgtg ctgccggcgg
     4081 cggttctgaa caagatgcag ttcatccaca ccgtgatcga cgaggtgggc ggctaggccg
     4141 gcatcacccg gcgttcgtcg ggaccccaca gcggctgcat gccgccgggc aacgccaact
     4201 gcgtcgcggt gatgacctca gccaggtcgc gcagctcgac gtggatcgca tcgaggagtt
     4261 ccgccaactt cgcgcgccgt gccccgccgc tcggggaggc ggaaaccgct tccagctcac
     4321 cgggatgcga ccggcgcacc agcgtgttga tctcgtccac caaccgctcc ggacgcgacg
     4381 accccgacga gccgggcagg tccttgaggt tggcccgcag ccgctccacc tgatacagca
     4441 acgaccgagg gttgttggcg tcgaaaagca tcagctcggc cacggcggcg acgctgacct
     4501 tgcccacggt gcggcgccgg tagatgaccg acgattcaca tgccaccagc gccgcctcga
     4561 tgatcgcctg ctcggcggcg gcgctgtgca catcggtcag cgtggcccgc aacagtgcgg
     4621 tcagccacag ggcgcgctcg atgcgtttgc cgatgtccat catcgtccag cccacgtcgc
     4681 gcaccatcga ctcgccggcc acccccgaca acgtcagcat ccccgacagt gtctgcgcct
     4741 gagccgacgc gagcagcacg tcggcttcgg cgagcgagtc cggcggttcg gaatccaacc
     4801 cgatcgcgcg ctccaccccg gcgagcacca tccacgtgtc gttggacagt tggtcgcgca
     4861 ccgcccgggc ggccagtgcc agcccgtcca ccgactggac aagggatccc ggccggctgg
     4921 ggtccacggt caacgaccac agcgtcgagg gggcgacggc gatcatctcg gcctggtcgc
     4981 cgtcgacccc ggtgtcggtc ccggtgatcc ggccgagcgc ggccatcagc accgggacgc
     5041 attcgctttc ttcggtgtcc tgatggtgcc ggtagacgtg gaagcggtcg cgggcgacga
     5101 tcagcaaccg cgccatccct tcggcgcgct cgctgtagcg ccccatccag aacaggtcgg
     5161 acagcacacg cggggagctg accccccagg tgccggcggc ggtcttcgct ggagccgcga
     5221 cggccgcctc caccgacgga acggtgaccg tctcggcacg cgcgcgcgcc gtcggccgca
     5281 cccagacatc ttttgcggca acggttttca gtgtgtacgc ggcgggcccg ggcgccagca
     5341 cgtagccgac gccaccgatc atcggcgcga agccgctgcg ttgggcgacg gtgaacagtc
     5401 gcataccgac cccggccgac gacaacacag cgccgtggtc ggtgggcgcc gacgagaatt
     5461 gcggcaattc ctggccgacc cagtgccacg gcatgtgctc gatctgcgct gccagctccg
     5521 tcagctgcgc ggacgaaagt gtcggtccga caagggtatt ctcgccgacg gtggacttga
     5581 tcagcagcga cgagagattg gccagcaggt gcgaccgttc gctggcgatg ccaccccagt
     5641 acaccggcgc ggaatgcagc aacggggctt cgcccagcag gcgcttggcc agctcgggca
     5701 gaaagcgcag cagcccaggg ctttccagga tgccactgcc cagcgtgttg acgacggtca
     5761 ccgtcccgcg ccgctgtgcc tccaccagac cgacgacgcc gagccgcgaa tcggcgcgca
     5821 gatccagcgg gtcggtgtat tccgcgtcga cccgccgcag caccacgtca acgcgtttca
     5881 gggtgcccag cgagcgcatc cacagcttgc cgtcgcgcac caccaaatcg gcgctttcca
     5941 ccagtggaaa gcccagcagg gtggccagat acgcctgatc gaaggcggtc tcggagtaga
     6001 tgcccgggct gagcaccacc acgacggggt cttgggcgac gtcgggtgcc gcgtcgatga
     6061 gcgccagccg caacatctgc gcgaacggtg tcgtgggtcg cggcgcgatg cgttcgtaga
     6121 ggtctggaat cgagtgcgcc accacgcgtc tgtcggccag cgcatagccg gcgcccgacg
     6181 gcgcctgcgt ccagtcggcg ttgacctgaa agctgccgtc cgacagccgg ctgagatcac
     6241 aggcatgcat gaaaagttgg tggtggccgg ggatctcgat gccgttggcg gcgcgcacgt
     6301 atccggggtg ggcgaacacc agctccggcg gtaacacgcc ttcggtcagc agggtgcgcc
     6361 gcccgtacag atcggtgagc acggcgtcga gcagccgcga ccgctgcacc agccccgtct
     6421 ccagcatgtc ccagtcggcc gcggacacca cgatcggcag ggtgtccagg ctccagggcc
     6481 gcggctccac cccgtgtccg ccgacggctt cgtcgcggtc cgaatcgacc tcggtgtagg
     6541 tgatgccgtc gttgtcgatc agactctcca ccaccgcgcg cagccggtcc agccctgccc
     6601 tgccgcgctc cgcgacggcg tcggccagct caccccatgc cggtcgcacg tttccttgcc
     6661 ggtcgacgaa ttcgtcgtac ccggttcccg ggccgtgacg cagatcgaac agcgcttcct
     6721 gggcccgcgc ggtccggtac ccggccaaca gccggtcggt gtcataagcg cccccggcaa
     6781 taccgggagc cgccgtcgcc tcactcatgc cgagtgccat tgctgtgcaa tcgtggacac
     6841 cattaccgct gcacggtacg cacgcgacgc aggtcgagaa tgccaggcgc gccgacgtcg
     6901 gtgaatatcc tggcctgcct ctcccggatg acggccaggt cgagcttgcc cggggtgaat
     6961 ccggtcgctt cgaagcggcg agcccggcgc gactcggccg cgacggcgtt gacgggcggc
     7021 tcgtcgtagg ccatgccgcc cgggtgcgcg acgtggtagg tgcagccgcc gcgcgacgtt
     7081 ccggaggtga ggtcgatcag ctcgaaccgc aggggtacat cggtggtgat ggtcgggtgc
     7141 agcgcgctgg gtggctgcca ggccttgaac cggacgccac caacgtggat gtcgggattg
     7201 tcggtggcca gcaacggcac cgggtagccg ttgaccgtca ccacgtagcg gtggcgatcg
     7261 gccccgatga tgcggatctg gatgcgttcg accgacgagt cgacgtagcg ggcggtgccg
     7321 gtggcgctgg cttcctcgcc gagggttttc cacggttcga tcgccccgcg cagctcgatc
     7381 tccaccccgt cgaacacggc ggtgccgatg cgcgggaagc ggaactcggt gaacgggtcc
     7441 agccagctgg tctcgaatgg gatgccgtgc gcccgcaggt cggcggccac gtcggcgatg
     7501 tcatgaatca agaagtgcgg caacaggtat cgcccgtgca ggttggcgcc gtggcggatc
     7561 agcggggcgc gcagcggttc gtcccagaac cacgcgacca aagagcgcac cagcagtgac
     7621 tggaccatcg ccatgcgcag atgcggtggc atctcgaagc cgcgcagctc gagcagcccg
     7681 agcctgccgc gggggctgtc ggggctgtag agcttgtcga tgcagaactc ggcgcgatgg
     7741 gtgttgccgg tgatatcggt gagcaggtgg cgcagcgcgc ggtcggtcac ccagggtttg
     7801 ggcgcgcccc ccgccgacag ccgggcgatc tcggcgaacg cgatctccag ctcgtagagg
     7861 gcgtcggagc cgccctcgtc gacccgcggt gcctgcgacg tggtgccgac gaatcgcccg
     7921 gcgaacaaat aagacaacga cgggtgccgc tgccagtagg tcagcagcga taccagcagg
     7981 tcgggacggc gcaatagcgg tgaatccgcg ggggtgacgc cgcccagcgt gatgtggttg
     8041 ccaccgccgg tgcccccgtg cgtgccgtcg atatcgaacg actccgttga taaccgagcg
     8101 aggcgagcct gtccgtagag cgtttccagc aactgggtct gttcggcgaa gctggcggtg
     8161 ggcgccacgt tgacctcgat gacgcccggg tcgggggtga tcgtcgtcga tgtcagccgc
     8221 gggtccggcg gtggcccgta gccctcgatg acgacggggc agtcggcttt ggccgccgcg
     8281 gcctccaccc ggccgatgag gtcgacgaag tggtcgagcg cctcggtggg cggaatgaag
     8341 atgtacagca gcccaccgcg cacctcggcg accagcgcgg tcggcggtgc cgtgtcggag
     8401 tcggtcacca ccgcatcgct ggcggcgggc tccgccgcca gctcgctccc cacggtcagg
     8461 gggtcggcgt cgagcgacgc tcttggcggc tgccagctga tcgaactcaa cggcagccgc
     8521 agacccgcgg gggaatcgcc ctcgatcagc acgatgcggc cccggcgcag ccgccagttg
     8581 gcgctggccc agcccagctc gtcgtcgcga cgatgcagcg gcagcacgaa agcggccggg
     8641 gcggaggtgg attcgtcgag gcgtgccagc agcgcggcgc ggcctgcctc gctatccgag
     8701 tgggcgtcgg agccggcttc gggggcgaga tcgtcgctgg gttccaccgg gtcgccggcg
     8761 ggcgtccgaa ccttggccgc cagccgggac aacgggtcct cgtaggcctg ccgcacctgc
     8821 gagagcggca gccccagatc gtcggcgatc ccggcgagca cctggtaggc cgaaccgtca
     8881 tcgaccggcg ccggcgggac gagctcggtg gcccaggggt cggccagcag ggtgtcgtcg
     8941 gtccacagcg gccgcccgtc ggtgcgccag tagagcgcaa tctgccagcg cggcaacggt
     9001 tctcccggat accaacggcc ctggctgcgc tggatcagcc cctgtggggc ccagaccgac
     9061 ttcaggcgca tggccaggtc ggatgcccgc tgtcgcttgt gcgggccgtc ggcggcggtc
     9121 gtccactcct cgtcggtctg gttgtccacg gacacgaacg tcggctcgcc gccgacggtc
     9181 agccggacgt cggcggcgtt cagtcgctcg tcgacgcgcc ggccgacgcc gcagattgtc
     9241 gcccacacct ggtcggtgta gggcagcgtg acgcgcggat cctcgtgcac gcgggtgacg
     9301 gtgttggaga actccagcac cgactcgcag ggttcggtgc cgccggtgat gggcgccgcc
     9361 gtcgaggggt gcggcgtggc cgcgagcggg atgtggccct cgccggcgaa cagcccggac
     9421 gtcgggtcca accctatcca gcccgcgccg gggatgtaca cctcggtcca cgcgtgcagg
     9481 tcggtgaagt cggcggcggg tcccgacggg ccgtcgagcg cctcgacgtc ggacgccagc
     9541 tgcaccaggt agccggacac gaaccgggcg gccagcccga actggcgcag gatcgacacc
     9601 agcagccacg ccgagtcccg gcacgatccg acgccggcgc gcaacgtgaa gtccggcgtc
     9661 tggacgccgg gctccatccg caggctgtag gtgacgtcgg cgttgatggc gcggttgagt
     9721 gagaccagga agtcgatggt gcgggtgcct tccggcaccg tgaagttgcg cagccacgcc
     9781 tgcacgagct cgccgggccc cgaaccttcg gcgtcttcgt cgacgggtcg caggtacggc
     9841 cgcaggtcgt cggccatctc cttggggtag atcagcccgc ccgccggcca tatctcggcc
     9901 cagtcctcga tgaagaagtc gaacgggttg accaccttga ggtcggcgat cagccccacg
     9961 gtgatggtca gccggttcat cgggccggga aacaccagcc gggccaggaa gttgcccacc
    10021 gcgtcctgtt gccagttgat gaaatgttcg cttggctcga cgcgcagcga gtaggcctcg
    10081 atagctgtgc gcgaatgggg cgccgggcgc agccgcacga cgtgcgggta aacccgaacc
    10141 agccggtcaa agacatagct ggtgcggtgc tccagcgcaa ctttgatgct catccgctga
    10201 tctcatcaca agcaccacaa ccccgcagcg cagcgcaatt aggcggagca cggcgatttc
    10261 agacgtgcaa aggcccgagc gccgttgcgg cgctcgggcc tttagccacg ggcgggcgct
    10321 atccggtgta ggggccgggc gggggtgccg gtgggtaacc gggctgctcg agcgcgacga
    10381 cctggccgcc cgagagcttg cggtaggtgt aggtcaggac gagcagcgcg acggggaggc
    10441 cgacgagcag cccgaccagg cacagcagtt cgccgacgag gacagctgcg atctgcacca
    10501 gccacgacag cagcgcgccg ccgacgttgg acctggtcgt ggcgaagctg gccttgatgg
    10561 agtcgatcgg cgaaagggat cggtcgacga cgaacgggat ggcgaacatc gcgaggaagc
    10621 cgaagacgat cccggggatg acgcagagca gcgttccgat ccaggtgccg atgccaacca
    10681 gcagggcggc caggataact ccacccaggt tgcgcggctt gaagaacgat ccgatcgtga
    10741 ccggcttgcc gtccgccaga tcgagggcgc ctgacagcaa tcctgcgtgc atgacgaccg
    10801 cgaccaggaa gatcaggatg tagccgacga ccatgacggc gatcgaggcg gtgccaagcg
    10861 tgacgctggt gctggcgctg gtctggccgt aggcgtcggt gtaggtggtg ctcgattgct
    10921 cgccgagggc cgcgggtagg aagctggtga ggccgccgac gatacccagg atcaggccgt
    10981 agatcagcac cggaacgacg agcgccgccg cgttggaggt gaacttgccc cacgcccagc
    11041 ggaacgcgtc accgacgctg aacgccgccg ggggcgggcc accgggacca ccgggaccgc
    11101 cgggaccgcc aaaaccaccg ggaccgccgg ggcctccggg gccataaccg ggcggcggcc
    11161 cgtagccagg tgggggcgcg ccgtagccgc cgggcggacc gggaggcggc ggaggcggcg
    11221 gtgcgccgta accgtgcggc gggccgggcg gcggggcgta cccgggcggc gggccgtaac
    11281 ccggtgccga gggaggcggc gggggtgggg gagcgccata gccgggcggt ggcgggtagc
    11341 ccggagggtt ctgatttccc ctgtgcgggt cgggcgggct gcctggatac tcgggaggct
    11401 ggctcatatc cgtcctagct gtcggattga ctctgtgggc acgcgcgcac ggaacttggc
    11461 aaaccggaac ttagcaaata agtggccagg gcgcgcgcat tggtggcgcc gcatcccggt
    11521 cggcgcgtcc tgtcgccccg acgtggtatg cctaaacccg tgtccgacgg tctgtttgac
    11581 gtccccggcc caccgcagac aacgaaccat gacctgggcg tgtcggccgg tgcgccgctc
    11641 gccgtccgga tgcgtccggc atcgctggac gaggtggtcg ggcaacgcca cctgctggca
    11701 ccccgatcac cgctgcgccg cctg
//
LOCUS       DASSGQ010000317         4146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_479412,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000317 DASSGQ010000000
VERSION     DASSGQ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479412"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /gene="cbiT"
                     /locus_tag="VFI55_13940"
     CDS             <1..596
                     /gene="cbiT"
                     /locus_tag="VFI55_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HET7075168.1"
                     /translation="VDDLNVIAVRYLPDERVSPLPDKVFVHDGQITKYGVRAVTLAAL
                     APRPGERLWDVGAGSGSIGVEWCLRWRGCSAVAFERDEGRRRNIGINAAAFGVRIDIR
                     GEAPDAFDDAQEPSAIFIGGGLTQAGLLDACLDNLPSGGRLVANAVTAESESLLAQAY
                     SRFGGQLRRFQHQQGEPLGAFTGWRPQMPVTQWAVTR"
     gene            593..1348
                     /gene="cobM"
                     /locus_tag="VFI55_13945"
     CDS             593..1348
                     /gene="cobM"
                     /locus_tag="VFI55_13945"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HET7075169.1"
                     /translation="MTVYFIGAGPGAADLITVRGQRLLERCPVCLYAGSIMPDDLLAL
                     CPPDAKIVDTGPLTLEQIIAELTDADAAGHDVARLHSGDPSLYSALAEQCRRLDSQGI
                     EYEIVPGVTAFAAAAAALKRELTVPGVAQTVTLTRVATLSTPMPPGEELAALARSTAT
                     LVLHLAAAQIDAVVPQLLEGGYKPETSAAVVAYASWPQQTVLRGTLADIARQMRDADI
                     TKTAVIIVGDVLAAEGFADSYLYSARRGRGSRH"
     gene            1345..2073
                     /locus_tag="VFI55_13950"
     CDS             1345..2073
                     /locus_tag="VFI55_13950"
                     /EC_number="1.3.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-6A reductase"
                     /protein_id="HET7075170.1"
                     /translation="MMRVLLLGGTAEARALAKTLHPQVDVISSLAGRVSDPALPVGPV
                     RIGGFGGVDGLRRWLQDEHIDAVVDATHPFAATMTAHAAQVCGQLQIPHVVLARPAWH
                     PGAAAIVASDTEAAEIVAQKCYSRVFLTTGRSGVNAFADSDAWFLIRAVTAPDAALLP
                     RRHRLLLSRGPYRYDDELALLRTHRIDALVTKNSGGDMTRAKLDAAAAQGIPVVMVAR
                     PQLPTGVTVVDTVQEAASWVAGLD"
     gene            complement(2070..2624)
                     /gene="sigC"
                     /locus_tag="VFI55_13955"
     CDS             complement(2070..2624)
                     /gene="sigC"
                     /locus_tag="VFI55_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigC"
                     /protein_id="HET7075171.1"
                     /translation="MTASGDDKAVTELALAAARGNARALEAFIKATQQDVWRFVTYLS
                     DAGSADDLTQETFLRAIGAIERFSGRSSARTWLLAIARRVVADHIRHAQSRPRTASGA
                     DPDQLPSGTRHARGFEDLVEVTTMIANLSAEQREALLLTQLLGLPYADAAEVCGCPVG
                     TIRSRVARARDALLADAERDDLTG"
     gene            complement(2742..3449)
                     /locus_tag="VFI55_13960"
                     /pseudo
     CDS             complement(2742..3449)
                     /locus_tag="VFI55_13960"
                     /inference="COORDINATES: protein motif:HMM:NF021550.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2275 domain-containing protein"
     gene            complement(3573..>4146)
                     /locus_tag="VFI55_13965"
     CDS             complement(3573..>4146)
                     /locus_tag="VFI55_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HET7075172.1"
                     /translation="DEPARARLACALAEEGRAVAVVSSGDPGVFAMATAVLEEAKQWP
                     GVRIRVIPAMTAAQAVASRVGAPLGHDYAVISLSDRLKPWEVIESRLAAAAAADLVLA
                     IYNPASATRTWQVGAMRDVLMAHREPGTPVVIGRSVSGPDEDVRVVRLADLDPAHVDM
                     RCLLIVGSSQTQWYSDDFGYRVFTPRRYPA"
BASE COUNT          659 a         1462 c         1403 g          622 t
ORIGIN      
        1 acgtcgatga cctgaacgtg atcgcggtcc gctacctgcc cgacgagcgg gtgtcgccac
       61 ttcccgacaa ggttttcgtc cacgacgggc agatcaccaa atacggggta cgcgcggtga
      121 ccctggcggc gctggcgccg cgacccgggg agcggctgtg ggacgtcggc gccggctcag
      181 gcagcatcgg tgtcgagtgg tgcctgcgct ggcgcggttg cagcgcggtg gcattcgagc
      241 gcgacgaggg gcgccgccgc aacatcggga taaacgccgc cgcgttcggc gtccgcatcg
      301 acatccgcgg cgaggcgccg gacgcgttcg acgacgccca agaaccgtcg gccatcttca
      361 tcggcggtgg cctgacccag gccggtttgc tcgacgcctg tctggacaac ttgccctccg
      421 gtggacggtt ggtcgccaac gcggtcaccg ccgaatccga atcacttcta gcacaagcat
      481 attcgcgctt cggcggccaa ctgcgacgct tccagcatca gcagggtgaa cccctgggcg
      541 ccttcacggg atggcgcccg cagatgccgg tcacccagtg ggcggtcacc cggtgaccgt
      601 gtacttcatc ggcgccggcc caggcgcggc cgacctcatc acggtgcggg gccaacgtct
      661 tctcgaaaga tgcccggtgt gcctctacgc gggttcgatc atgcccgacg acctgctggc
      721 gctgtgcccg cccgacgcga aaatcgttga caccgggccg ttgacgctcg agcagatcat
      781 cgccgaactc accgacgccg acgccgccgg ccacgacgtg gcgaggctgc attccggcga
      841 cccgtcgctg tacagcgcgc tggccgagca gtgccggcga ctcgactcgc agggcatcga
      901 atacgaaatc gtgccgggtg taacggcttt cgccgcggcg gcggcggccc tgaaacgcga
      961 gctcaccgtg cccggagtgg cccagacggt gacgctgacc cgggtggcga ccctgtcgac
     1021 acccatgccg cctggcgaag agctggctgc cctcgcccga tccaccgcca ccctggtgct
     1081 gcatctggcc gccgcccaaa tcgacgccgt cgtcccgcaa ctactcgaag gcggctataa
     1141 acccgaaaca tccgccgcgg tcgtggctta cgccagctgg ccacagcaga ccgtgctgcg
     1201 tggcacgctt gccgacatcg cccggcagat gcgcgacgcc gacatcacca agaccgccgt
     1261 gatcatcgtt ggcgatgtgc tggcggcgga gggattcgcc gacagctacc tgtattcggc
     1321 ccggcggggt cgggggagcc ggcactgatg cgggtgctgc tgctcggcgg caccgccgag
     1381 gcccgcgcgc tggcgaaaac cctgcacccg caagtcgacg tcatcagctc gctggccggg
     1441 agggtatcgg acccggccct gcccgtcggc ccggtccgca tcggcggttt cggcggtgtc
     1501 gacgggctgc gccgctggct gcaagacgaa cacatcgacg ccgtcgtcga cgccacccac
     1561 cccttcgcgg caaccatgac agcgcacgcc gcgcaggtgt gtggccaact acagataccc
     1621 cacgtggtgc tggcccggcc ggcctggcac cccggcgcgg ccgcaatcgt ggcatcggac
     1681 acagaagcgg cggaaatcgt tgcgcagaaa tgttattcgc gcgtcttcct gaccaccggt
     1741 cgctccggcg tgaacgcctt cgccgacagt gacgcgtggt ttttgatccg cgccgtcacc
     1801 gcgcccgacg ccgccttact cccgcgtcgt caccggctgt tgctgtcgcg cgggccgtat
     1861 cgctacgacg acgaactggc gctgctgcgc acgcaccgca ttgacgcgct ggtgaccaag
     1921 aacagcggcg gcgacatgac tcgtgcaaag ctggacgccg ctgcggcgca gggcattccg
     1981 gtggtgatgg ttgcccgccc gcagctgccg actggagtga cggtggtgga cacagttcaa
     2041 gaggcggcca gctgggtcgc cgggctggat tagccggtga ggtcgtcgcg ttcggcgtcg
     2101 gccagcagcg catcccgggc gcgcgccacc cgagagcgga tggtgcccac cgggcaaccg
     2161 cacacctcgg cggcgtcggc gtagggcagt ccgagcagct gggtgagcaa caacgcttcc
     2221 cgttgttcgg cgctcaggtt ggcgatcatc gtggtcacct caaccaggtc ttcgaacccg
     2281 cgggcgtgcc gcgttccgct gggcaactgg tcggggtcgg cgcccgatgc ggtgcgcggc
     2341 cgcgattggg cgtggcggat gtgatcggcg acgacgcggc gcgcgatggc cagcagccag
     2401 gttcgggcac tggaccgtcc cgagaaccgc tcgatggcgc cgatcgcccg aaggaaggtt
     2461 tcttgggtga gatcgtcggc gctgcccgca tccgacaggt aggtgacgaa ccgccagacg
     2521 tcttgctgag tcgctttgat aaacgcctcg agcgctcgcg cgttcccgcg tgcggcggcc
     2581 aaggcgagtt cggtgacggc cttgtcgtcg cctgacgcgg tcatgagcaa ccaccttagt
     2641 tgcgcgggct cggtcagtct ccgaccggtc gtcgtagacg cgttgacggg gtcgggagcc
     2701 gaccggttga tcggccagag gtgcccgcgc cgtcgagcgg tgtgcggcgc ggtggtctgg
     2761 gcggggcggt cgacttcggc gtcggcgtcc gaactgcgcg gccggcgcga ccccgcgcgt
     2821 tcgcgcgcca cgagcaacgt gaacaccaag ccgagcaaca cgggcaggtg actggcgata
     2881 cgtgccgggg tgtcctcgcc ggccaaggcg tcaacaacct catagcccac caacgcaacg
     2941 gtgtacacgg cggtgatagc cgcgacgccg atcgcggcga ccggccatat cccggcggcg
     3001 atcgtcgcca aacccaacgc cagcaaccat gcggtcgact cgtgcatcag gtgctccccg
     3061 gacatcgcgc cgtgctcgtg ctccgacacc atgccgaaat cgatcccgct gatttgggcg
     3121 gccgcgatcg ccacctgaaa aagccccacg ccgatcagcc cacaccgcag atagtgcgtc
     3181 cgcagccagc gcatccagcg gtgataagcg gtggactgct cggccaggct tgccatgatc
     3241 ttgtcaacca ggtccggccc caggccgggt tcgatcgagg ccaggcggcg agtctgcacc
     3301 gcagcgccga tcaaccaggt gcggcaaccc cggcacgatt ccaggtgggc atcgacctgc
     3361 tgcgcgagca cctgcggacg ctcgccgtcc aatcgtgcag aaagcgcctc gcgcgcaacc
     3421 tcacaccgca tccctgcatc gttgcgcatg cccggtccac gcgcaaagac cgggcggact
     3481 cacgcttcgc ccggctccgc cgagctggcg atcaccacta gccgattggt ggtgatccgc
     3541 ttcgcccggc tccgccgagc tagcgatcac cactaggccg gatagcgccg aggggtgaac
     3601 acccggtatc cgaaatcgtc ggaataccac tgggtttgcg aggacccgac gatcagcagg
     3661 cagcgcatgt cgacatgggc ggggtcgagg tcggccagcc gcaccacgcg cacgtcctcg
     3721 tcggggcccg acaccgagcg cccaatcacc accggtgtgc cgggttcgcg gtgggccatc
     3781 agcacgtcgc gcatcgcgcc gacctgccag gtccgcgtcg ccgacgccgg gttgtagatg
     3841 gccagcacca gatcggcggc ggccgcggcg gccagccgcg actcgataac ctcccatggt
     3901 ttgagccggt cggacaacga gatcaccgcg tagtcgtggc ccaacggggc accgactcgg
     3961 ctggcgacgg cctgggcggc ggtcatcgcc ggaatcaccc ggatccgcac cccgggccat
     4021 tgtttggcct cttccaggac ggcggtggcc atcgcgaaca ctcccggatc gcccgacgac
     4081 accaccgcga cggcgcgccc ttcttcggcc agcgcgcagg ccagccgcgc ccgcgcgggt
     4141 tcgtcg
//
LOCUS       DASSGQ010000318         5251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_53848, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000318 DASSGQ010000000
VERSION     DASSGQ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53848"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..678)
                     /gene="upp"
                     /locus_tag="VFI55_13970"
     CDS             complement(55..678)
                     /gene="upp"
                     /locus_tag="VFI55_13970"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET7075173.1"
                     /translation="MEVRVVDHPLAVARLTTLRDERTDKAGFRAALRDLTVMLVYEAT
                     RDAARETYPVRTPLAETLGVRLANPPLLVPVLRAGLGMVDQALALLPEARVGFVGVAR
                     DEETHQPRGYMESLPDDLTALPVMVLDPMLATGGSLKHTIDLLHRRGASDITVLCAVA
                     APEGIAALEESAPNARLFAVAVDDGLNEVAYIVPGLGDAGDRQYGPR"
     gene            855..1676
                     /locus_tag="VFI55_13975"
     CDS             855..1676
                     /locus_tag="VFI55_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET7075174.1"
                     /translation="MALTGLAATALAPRASLAAAAIPQPAHVVIVVEENRSEGNIIGN
                     KSAPFISALAANGANMAESFAETHPSEPNYLALFAGSTFGVTKDLCPVNGGNLPNLAS
                     ELLAAGYTFIGYAEDLPAVGSPVCSAGKYARKHVPWANFTNVPPANSVPFSAFPMGNY
                     ANLPTVSFVIPNNNDNMHDGSIAQGDAWLSRQLSGYANWAVANNSLLVVTWDEDDDSA
                     RNQIPTVVYGAHVQPGTYNDQINHYNVLSTLEQMYGLPKTGNAVNAAPIADIWTA"
     gene            1760..3130
                     /locus_tag="VFI55_13980"
     CDS             1760..3130
                     /locus_tag="VFI55_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein"
                     /protein_id="HET7075175.1"
                     /translation="MAADLVPIRLSLSAGDRYTVWAPRWRDAGDEWEAFLGRDEDLYA
                     FETVADLIAFVRSDTENDLVDHPAWDDLTSAPSRDFQPDEDRQFDLVVVEELLSEKPT
                     QESVNALAAALAIVSSIGSVCELAAVSKFFNGNPTLGTVSGGIDHFTGKAGQKRWNAI
                     AEVVGRSWDGVISAIDAIITAPEVDAALSAKAAEELAEELEDEATEDDEEAAEDSENE
                     DDTSEADEEDAADETDDSEDSDDSEDEEDEEDEEDEDSEDEEDEARATGDTVVLGTDE
                     DFWLQVGIDPIRIMTSSGTFYTLRCYLDDRPIFLGRNGRISVFGSERALARYLADEHD
                     HDLADLSTYDDIRTAATDGSLRVEVTDENIYVLSGLADDFADGPDSVDRDQLDLVVEL
                     LRDIGDYSEEGTVDKTLEPGRPLGKLVAYVLEPDSVAKPGPPYAATVREWEKLEQFVD
                     GRLRRE"
     gene            3245..4228
                     /locus_tag="VFI55_13985"
     CDS             3245..4228
                     /locus_tag="VFI55_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7075176.1"
                     /translation="MSLPGIGPLPLYGFQRPGMLLFGLIPLALLAVYVIVQARRRRRL
                     RRFTESEVPQSWWRHLPIAASLLCLVLLTIALATPTHDMRIPRNRAVIMLVIDMSQSM
                     RATDVEPNRLKAAEQAATQFASQLTPGINLGLVGFAGTPYLLVPPTPQHQATVEALKK
                     LDFADSTATGEAIFTALHAVGATAVAGGDSPPPARIVLLSDGAENKPSDPNDPHDGAY
                     TAARLAKDQGVPISTISFGTPTGEIEMDGQRVAVPVQTDQMRTIARLSGGQSYSATNV
                     GELNKSYNAIEKDIGYRTVPGPGSAGWLRLGVITALIATMLALVINRRLPT"
     gene            complement(4233..5240)
                     /locus_tag="VFI55_13990"
     CDS             complement(4233..5240)
                     /locus_tag="VFI55_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7075177.1"
                     /translation="MKLPLLGPVSLTGFQNAWFFLFLLLVLLVIGVYVAVQFARRRRV
                     LRFANMEVLERVAPIRSGRWRHLPTILLATSLVLLTTAMAGPTSDIRIPLNRAVVMLV
                     IDISESMASTDVPPNRLAAAQEAGKKFADELTPAINLGLVEFAANASLLVAPTTNREA
                     VKAAIDSLKPAPKTATGEGIFTALQAIATVGSVMGGGDGPPPARIVLESDGAENVPLD
                     PNAPQGAFTAARAAKSAGVQISTISFGTPYGTVDYEGATIPVPVDDQTLQKICEITDG
                     EAFHADSLESLKQVYSTLQRQIGYETVKGDASLAWMLLGTVVMAGAVLAGLFLNSRLP
                     S"
BASE COUNT          935 a         1775 c         1718 g          823 t
ORIGIN      
        1 cgcgaccaat tggtctccgc ggtgcaaggc tggtgagcca ggcgcggcac caggctagcg
       61 ggggccgtat tggcggtcac ccgcatcgcc gagacccggc acgatgtagg cgacctcgtt
      121 gagcccgtcg tcgacggcca cggcgaacag ccgcgcgttc ggcgccgatt cctcaagtgc
      181 cgcaatgcct tccggggcgg ccaccgcaca cagcaccgtg atgtccgatg cgccgcggcg
      241 gtgcagcaaa tcgatcgtgt gcttcagtga cccgcccgtc gccagcatgg ggtcgagcac
      301 cataacgggc aaagcggtca ggtcgtccgg cagcgactcc atgtagcccc tcggctgatg
      361 ggtttcctcg tcgcgagcca cgccgacgaa tccgacccgg gcctccggca gcagcgcgag
      421 cgcctggtcg accataccga gcccggcgcg caacaccggc accagcagag gcgggttggc
      481 cagtcgaacc ccgagcgttt cggccagcgg tgtgcggact gggtaagtct cgcgggcggc
      541 gtcgcgcgtg gcctcataca ccagcatcac ggtcaggtca cgcaacgcgg cccggaagcc
      601 ggccttgtcc gtgcgttcgt cacgcaacgt ggtcagccgg gcgaccgcca gcgggtgatc
      661 gacgacacgc acctccacgg gattcgaccc tatataacaa tccggctccg gatcgttaaa
      721 ggcccgctga cacgctcgcg tcggcgccgt cgacgcgaga aatcggctca tatactcccc
      781 gggtgccgag cccaatcgaa ggtccaagaa ggttgataga cagggcgtat cgagcgctcc
      841 gcctggtcgg cgcggtggcg ttgacggggc tggcggccac cgcgctggcg cctcgggcga
      901 gccttgcggc ggccgcgatc ccgcagcccg cacatgtggt catcgtggtg gaagagaacc
      961 gctctgaggg caacatcatc gggaacaagt cggcgccctt catcagcgcg ctggccgcca
     1021 atggcgcgaa catggccgag tcgttcgccg aaacacatcc cagcgagccg aactatctgg
     1081 cgctcttcgc cggcagcacg ttcggcgtga ccaaggacct gtgcccggtc aacggcggca
     1141 acctgcccaa cctggcttct gaattgctcg ccgcgggcta cacgttcatc ggctatgccg
     1201 aagaccttcc ggcggtcggc tcacccgtgt gcagcgccgg caagtacgcg cgcaagcatg
     1261 tgccgtgggc caacttcacc aacgtgccgc ccgcgaactc ggtgccgttt tctgcgttcc
     1321 cgatgggaaa ttacgccaac ctgcccaccg tgtcgttcgt cattcccaac aacaacgaca
     1381 acatgcacga cggttcgatc gcgcagggcg atgcctggct gagccgccag ctgtcggggt
     1441 acgccaactg ggcggtggcc aataacagcc tgctcgtcgt gacgtgggac gaggacgacg
     1501 actccgcccg caaccagatc ccgacggtgg tttacggagc ccatgtgcag cccggcacct
     1561 ataacgacca gatcaaccac tacaacgtgc tctccactct cgagcagatg tacgggctac
     1621 ccaagacggg taacgcggtg aacgctgccc cgatcgccga tatctggacc gcctaggccc
     1681 gccgtcccgg cgctcgctgt cgccgggcta gccgcatccg ggcccggcac cttcgtcgtc
     1741 cgtcgctatt ctgtgccgca tggctgctga cctcgtgccc atccgcctga gcctgtccgc
     1801 cggtgaccgc tacaccgtgt gggcgccccg ctggcgcgac gccggcgacg agtgggaggc
     1861 ctttctgggc agggacgagg acctgtatgc cttcgagacc gtcgccgacc tgatcgcgtt
     1921 tgtgcgctcc gacaccgaaa acgatctggt cgaccatccg gcgtgggacg acctgacctc
     1981 ggctccctcg cgcgatttcc agccggacga agacaggcag ttcgatctgg tcgtcgtcga
     2041 ggagctgtta tcggagaagc cgacccagga gtcggtgaat gcgctcgccg ccgcactggc
     2101 gatcgtgtcg tccatcggct cggtgtgcga actggcggcg gtgtcgaagt tcttcaacgg
     2161 caaccccact ctgggcacgg tgtccggcgg catcgaccac ttcaccggca aagccggcca
     2221 gaaacgctgg aatgcgatcg cagaggtcgt cgggcgaagc tgggacggcg tcatcagcgc
     2281 gatcgacgcg atcatcaccg ccccggaggt cgatgccgcg ttgtcggcca aggcggccga
     2341 ggaactggcc gaagagctcg aggacgaggc gaccgaagac gacgaggagg ccgctgagga
     2401 ctccgaaaac gaagacgaca ccagcgaggc agacgaggaa gacgcggccg acgaaaccga
     2461 cgactccgaa gactccgacg actccgaaga cgaagaagac gaagaagacg aagaagacga
     2521 agactctgag gacgaggaag acgaagcccg cgctaccggg gacaccgttg tcctgggcac
     2581 cgacgaggac ttctggctgc aggtgggcat cgacccgatt cgcatcatga ccagttccgg
     2641 gacgttctac acgcttcgct gctacctgga cgatcgcccg atctttctgg gccgcaacgg
     2701 acgaatcagc gtcttcggct ccgagcgggc gctggcccgc tacctggccg acgagcacga
     2761 ccacgacctg gccgatttga gcacgtacga cgacattcgt accgccgcaa ccgacggctc
     2821 cttgcgggtc gaggtcaccg acgagaacat ctacgtgctc agcggtttgg cggacgactt
     2881 cgccgacggg ccggactcgg tggatcgtga ccagctcgac ctggtcgtcg agctgctccg
     2941 cgacatcggg gactactccg aggagggcac ggtggacaag acgctcgagc cgggccggcc
     3001 gctgggcaag ttggtggcct acgtgctcga acccgactcg gtcgccaagc cggggccccc
     3061 gtacgcggcc acggtgcgtg agtgggagaa gttggagcag ttcgtcgacg ggcggctcag
     3121 gcgcgaatag ccgccacgtt tgacgcgcct tgaagactgc tctttactgg cgggcagcgt
     3181 ccgccgtccg tcgaggggac ggccggaacg ggtctagacg ggtctgaacg ggaaggtaaa
     3241 gcacgtgtca cttcccggaa ttggcccgtt gccgttgtac gggttccaac gtccgggcat
     3301 gctgctgttc ggtttgatcc cgctggctct gctcgccgtt tacgtcatcg tccaggctcg
     3361 acgccggcgt cggttgcgcc ggttcaccga gtcggaggtg ccgcagtcgt ggtggcggca
     3421 ccttccgatc gcggcgtcgc tgctctgcct ggtgctgttg acgatcgcgt tggccactcc
     3481 cacccacgac atgcgcatac cgcgcaatcg cgcggtcatc atgctggtca tcgacatgtc
     3541 gcagtccatg cgggcaaccg acgtcgagcc caaccggctg aaggcggccg aacaggccgc
     3601 cacccagttc gccagtcagc tgacgccggg catcaacctc gggctcgtcg gcttcgcggg
     3661 aacgccctac ctgctggttc ccccgacccc gcagcaccaa gcgacagtgg aagcgctgaa
     3721 aaagctcgac ttcgccgaca gcacggcgac cggcgaggcc atcttcaccg ccctgcatgc
     3781 ggtcggggcc accgccgtcg cgggcgggga cagtccgccg ccggcccgca tcgtgctcct
     3841 ctccgacggc gccgagaaca agccgtcgga tcccaacgat ccgcacgacg gcgcctacac
     3901 cgccgcacgc ctggccaagg accagggcgt gcccatctcg acgatctcgt tcggcacccc
     3961 gacgggcgag atcgagatgg acggacagcg ggtcgccgtc ccggttcaga ccgaccagat
     4021 gagaaccatc gcccggctgt ccggcgggca gtcctatagc gccaccaacg tcggcgaact
     4081 caacaagagc tacaacgcca tcgagaaaga catcgggtat cgcaccgtgc ccggtcccgg
     4141 cagtgccggg tggctgcgcc tcggcgtcat caccgccctg atcgcgacga tgctggcgct
     4201 ggtgatcaat cggcggcttc cgacctgatc gctcaggacg gcagcctgct gttcaggaac
     4261 agcccggcca gcacggcacc ggccatgacc accgtcccga gcagcatcca ggcgaggctc
     4321 gcgtcgccct tcacggtttc gtagccgatc tggcgttgca gcgtcgaata gacctgcttg
     4381 agcgactcca ggctgtcggc gtggaacgcc tcgccgtcgg tgatctcaca gattttctgc
     4441 agcgtctgat cgtccaccgg aaccggaatg gtggcgccct cgtagtcgac ggtgccgtag
     4501 ggcgtgccga aggagatcgt cgatatctgc actcccgcgg atttcgcggc ccgggccgcg
     4561 gtgaacgcgc cctgcggcgc gttggggtca agcggcacgt tttcggcgcc gtcggactcc
     4621 agcacgatcc gtgcgggtgg cggcccgtcg ccccctccca tcaccgagcc gaccgtggcg
     4681 atggcctgta gcgcggtgaa gatgccctca cccgtggcgg ttttcggggc cggcttgagg
     4741 ctgtctatcg ccgctttgac ggcctcacga ttggtcgtcg gggcgaccaa cagtgaggcg
     4801 ttggcagcga actccaccag ccccaggttg atagccggcg tcagctcgtc ggcgaatttc
     4861 ttgccggcct cctgggccgc agccagccgg ttgggcggga catcggtgga cgccatcgac
     4921 tccgagatgt cgatgaccaa catgacgacc gcgcggttga gcggtatccg gatgtcagac
     4981 gtcggcccgg ccatcgcggt ggtcagcagc accaacgacg tggcaagcag gatcgtcggc
     5041 aggtgccgcc accgacccga gcggatcggc gcgacgcgct cgagtacttc catgttggcg
     5101 aaccgcagta cccgccgccg gcgggcgaat tgcaccgcga cgtagacgcc gatgaccagc
     5161 agcacgagca acaggaacaa gaagaaccag gcgttctgga agccggtgag cgacacggga
     5221 cccagcaggg gcaacttcat gtccagccac a
//
LOCUS       DASSGQ010000319         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_540238,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000319 DASSGQ010000000
VERSION     DASSGQ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540238"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VFI55_13995"
     CDS             <1..542
                     /locus_tag="VFI55_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HET7075178.1"
                     /translation="ELPWLRRKRIQEDWGISDEVMRDLVNAGAVELVAATVDQGASSD
                     AARAWWGNFLVQKANEAGVELDALPITPAQVAAVVKLIDDGKLSNKLARQVVEGVLAG
                     EGEPEQVMKDRGLEVVRDDSALQAAVDEALAANPDVVEKIRGGKVQAAGAIVGAVMKA
                     TKGQADAARVRELVLAACT"
     gene            708..2003
                     /locus_tag="VFI55_14000"
     CDS             708..2003
                     /locus_tag="VFI55_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HET7075179.1"
                     /translation="MRNASLWRALLGVESTVVEDVEFDEGAQVLVAHVRPRRANRGRC
                     GTCGSRASWYDRGQGRRRWRGLDMGPIQVFLEADVPRVNCPAHGPTVRQVPWARHGAG
                     HTRSFDQQVAWLATQCSKRAITELMRIAWRTVGVIITRVWADTAAGVDAFAGLQRIGI
                     DEISYKRRHKYLTVVVDHDSGRLVWAHPGRERATVGAFFDALEASGQGRCAQITHVTA
                     DGAEWIADVVSKRCPNAIRCADPFHVVGWATEALDAVRREAWNNARRDGHTRNHGWAH
                     GRRATVATGPAQALRRARYALWKNPENLTDRQQVKLDWIAKTDPRLYRAYLLKEGLRT
                     VFKLPADQAGEALDRWVAWARRCRIESFVTLQRRIVGHRAQILASIAHGLSNGLIESV
                     NTKIRLITRIAFGFAHPGPLIALAMLTLGGHRPALPGRQ"
     gene            complement(2059..>2568)
                     /locus_tag="VFI55_14005"
     CDS             complement(2059..>2568)
                     /locus_tag="VFI55_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HET7075180.1"
                     /translation="GMVRMLRCAGAALDDGMVYWDVRASARFPTIEVRVADVPATVAE
                     TVLLATLIRASVMTALDDERRGEPTPPLTPHALKAAYWKSARDGLDGKAIDLLESHAP
                     VPARKLLQRLVDHVRPALEDVGDYKMVKAELARLDYVGNGAMRQRRAWDRQHDVKDVI
                     AELAAATLS"
BASE COUNT          432 a          885 c          856 g          395 t
ORIGIN      
        1 ccgagctacc gtggttgcgg cggaagcgga ttcaggagga ctgggggatc tccgacgagg
       61 tgatgcgtga cctggtcaac gccggcgccg tcgaattggt cgccgccacc gtcgatcagg
      121 gcgcgtcgag cgacgcggcc cgcgcatggt ggggaaactt cttggttcag aaggccaatg
      181 aggctggcgt cgaactcgac gcgctgccga tcacgccggc gcaggtcgcc gccgtcgtga
      241 agctcatcga cgacggcaag ctgtccaaca agctggcgcg ccaggtcgtc gagggagtgc
      301 tggccggcga gggcgagccc gagcaggtga tgaaggaccg cgggctggaa gtcgtacgcg
      361 acgattcggc gctgcaggcc gcagtcgatg aggcgctggc cgccaacccc gacgtcgtcg
      421 agaagatccg cggcggcaag gtgcaggcgg cgggcgccat cgtcggtgcg gtgatgaagg
      481 cgaccaaggg ccaggccgac gccgcgcggg tgcgcgagct ggtgttggcc gcctgtacct
      541 agcgccgacg ggcgtgtcgc cggtgggcgt ctcttctgcg cgccgtcatc aaatgtggct
      601 cttgtgacac ttccgtgggt cgttgagtgc gcctgaacgg ccacacgccg ttgtccgtct
      661 aggtttttgg cttgtcgaag gtcaaaaact acagatcgga gaacggcgtg cggaacgcca
      721 gtttatggcg cgccttgttg ggcgtcgaga gcaccgtggt cgaggacgtc gagttcgacg
      781 agggggcaca ggttctcgtg gcccacgtgc ggccgcgacg agcgaaccgg ggtcgatgcg
      841 gaacctgcgg atcgagggcc tcctggtatg accgtggcca ggggcggcgg cggtggcgcg
      901 ggctcgacat gggacccatc caggtcttcc tggaagctga cgtcccgcgg gtcaactgcc
      961 ccgcccacgg gccgacggtg cgacaggtcc cctgggcccg acacggcgcg gggcataccc
     1021 gctcgttcga tcagcaggtg gcctggttgg ccacgcagtg ctcgaagcga gcaatcaccg
     1081 agctgatgcg gatcgcctgg cgcacagtcg gcgtgatcat cactcgggtg tgggccgaca
     1141 ccgccgccgg ggtcgacgcg ttcgccggcc tgcagcggat cgggatcgat gagatctcct
     1201 acaagcgccg ccacaaatac ctgaccgtcg tggtcgacca cgacagcggc cggctggtgt
     1261 gggcgcatcc cggccgcgaa cgcgcgaccg tgggcgcctt cttcgatgcg ctggaggcat
     1321 cggggcaggg tcgatgcgcc cagatcaccc acgtcaccgc cgacggggcc gaatggatcg
     1381 ccgacgtggt cagcaagcgc tgccccaacg ccatccgatg cgccgacccg tttcacgtcg
     1441 tcggctgggc caccgaggca ctcgatgccg tgcggcgcga ggcgtggaac aacgcccgcc
     1501 gcgacggtca cacccgcaat cacgggtggg cccatggccg acgcgccacc gtggccaccg
     1561 gaccagccca agcactgcgg cgcgcacggt atgcgctgtg gaagaaccca gagaacctca
     1621 ccgaccgcca acaggtgaag ctggactgga tcgccaaaac cgacccgcgg ctctaccgcg
     1681 cctacctcct caaggaaggc ctgcgcacgg tcttcaaact gcccgccgac caggccggcg
     1741 aagccttgga caggtgggtc gcctgggcac ggcgctgccg catcgaatca ttcgtcaccc
     1801 tccaacgtcg catcgttggc caccgcgcgc agatcctcgc ctccatcgcc cacggcctat
     1861 ccaacgggct catcgaatcg gtcaacacca agatccgact catcacccgc atcgccttcg
     1921 gcttcgctca ccccggcccc ttaatcgccc tggcaatgct caccctagga gggcaccgcc
     1981 cagccctgcc cggccgtcaa tgacccacag atcagtcagc agagcctcat ctgttgacgg
     2041 tccgcagaag cactattcct acgacagcgt ggcggccgcc aactcagcga tgacgtcttt
     2101 cacgtcatgc tgtcggtccc atgcccgccg ttgccgcatc gcgccgttgc ccacatagtc
     2161 gaggcgcgcc agctcggcct tcaccatctt gtagtcgccg acgtcttcga gggccggcct
     2221 gacgtgatcg accaagcgct gcaacaactt tcgcgcgggc accggcgcat ggctttcgag
     2281 cagatcgatg gctttgccgt caagcccgtc gcgggccgac ttccagtacg ccgccttgag
     2341 cgcgtgcggc gtgagcggcg gcgtgggttc gccacgtcgc tcgtcgtcga gcgccgtcat
     2401 cacgctggcc cgaatcaacg tggccaacaa gaccgtctcg gcgaccgtcg cgggcacgtc
     2461 agccacgcgc acctcgatcg tcgggaacct cgccgacgcg cggacatccc aatagaccat
     2521 gccgtcgtcg agcgccgccc cggcgcagcg caacatccgc accatccc
//
LOCUS       DASSGQ010000320         7253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_904414,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000320 DASSGQ010000000
VERSION     DASSGQ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904414"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VFI55_14010"
     CDS             <1..694
                     /locus_tag="VFI55_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7075181.1"
                     /translation="NLGVALSGLGGSLSTALSLGLSGSLSGLGPLSALLEGGLTGGLS
                     GLATTGISLANAFGAGLNGLVQTGASLVANVGAELANLNLGLSINAALSASLNAALSG
                     LGQTVQALTAGLGGGLGGLIHTGEALGGSLAASLSDLGATGNALVRVWEDAGAEIGSA
                     LSGALSGSIGVAFPGFIGAGAALAAGVAGASAGLEQTGGSLVTSLDVALTDLELSLQA
                     ALSASLGVGVAA"
     gene            844..1887
                     /gene="argC"
                     /locus_tag="VFI55_14015"
     CDS             844..1887
                     /gene="argC"
                     /locus_tag="VFI55_14015"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET7075182.1"
                     /translation="MADLLRVAVAGATGYAGGEILRLLLGHPACADGRLTIGAVTAAA
                     SAGSTLGEHHPHLIPLAQRVVEPTELAVLAGHDVVFLALPHGHSAALAEQLSPETLIV
                     DCGADFRLTDAATWERFYGSPHAGSWPYGLPELPGGRERLRGARRIAVPGCYPTAALL
                     ALAPAVAGDLIEPAVTVVAVSGTSGAGRAAKADLLGSEVIGSARAYSIAGAHRHTPEI
                     AQGLRAVTDRDVTVSFTPVLIPTSRGILATCTARTRSPLSQLRAAYEKAYDAEPFIYL
                     MPEGQLPRTGAVTGSNAAHIAVAVDEAAETFVAIAAIDNLVKGTGGAAVQSMNLALGW
                     PETEGLSVIGVAP"
     gene            1884..3098
                     /gene="argJ"
                     /locus_tag="VFI55_14020"
     CDS             1884..3098
                     /gene="argJ"
                     /locus_tag="VFI55_14020"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HET7075183.1"
                     /translation="MTEIATAARLLREQGVTAPAGFRAAGIAAGIKASGARDLALVFN
                     EGPDYAAAGVFTRNQVKAAPVLWTRQVLTTGRLRAVILNSGGANACTGPAGFQDTHAT
                     AEVVAAALSDWGTETGAIEVAVCSTGLIGDRLPMDKVLTGVREIVREMAGGLNGGEDA
                     AHAIMTTDTVPKQVALHHLDNWTVGGMAKGAGMLAPSLATMLCVLTTDAAVEPAALDR
                     ALRRASARTFDRLDIDGSCSTNDTVLLLASGASGITPPQADLDDAVLRVCDDLCAQLQ
                     ADAEGVTKRVSVTVMGAASDDDALTAARVIARDSLVKTAVFGSDPNWGRVLAAVGMAP
                     ITLDPDRITVSFNGSAVCVDGAGAPGAREVDLSGADIDINVDLGVGEGRATIRTTDLS
                     HAYVEENSAYSS"
     gene            3095..3982
                     /gene="argB"
                     /locus_tag="VFI55_14025"
     CDS             3095..3982
                     /gene="argB"
                     /locus_tag="VFI55_14025"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HET7075184.1"
                     /translation="MNTKTEALPTQVKAQVLAEALPWLKQLHGKIVVVKYGGNAMTDD
                     ALRHAFAADMAFLRNCGIHPVVVHGGGPQITAMLRRLGIEGDFKGGFRVTTPEVLDVA
                     RMVLFGQVGRELVNLINAHGPYAVGITGEDAQLFTAVRRSVTVDGVATDIGLVGDVEL
                     VNTAAVLDLIAAGRIPVVSTLAPDAEGVVHNINADTAAAALAEALGAEKLLMLTDVEG
                     VYASWPDRDSLVSEIDTATLAQLLPTMEAGMIPKVEACLRAVTGGVPSAHVIDGRVEH
                     CVLVELFTDAGTGTKVVSV"
     gene            4040..5230
                     /locus_tag="VFI55_14030"
     CDS             4040..5230
                     /locus_tag="VFI55_14030"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HET7075185.1"
                     /translation="MTNTDTMKHRWESVMMNNYGTPPVALASGDGAVVTDVDGKTYLD
                     LLGGIAVNVLGHRHPAVIDAVTQQMSTLGHTSNLYATEPGIALAEELVALLGTDRQTR
                     VFFCNSGTEANELAFKLSRLTGRTKLVAAQEAFHGRTMGSLALTGQPTKQAPFEPLPG
                     DVTHVPYGDTEALAAAVDDDTAAVFLEPIMGESGVVVPPEGYLATVRDITARHGTLLV
                     LDEVQTGMGRTGAFFAHQHDGITPDVVTMAKGLGGGLPIGACLAVGPAAELLTPGLHG
                     STFGGNPICAAAALAVLRVLAAEDLVRHAEVLGKSVRHGIETLGHPLIDHVRGRGLLW
                     GVVLTAPLAKDTEAAARDAGFLVNAAAPDVIRLAPPLIITDTQIDGFIAALPGILDAA
                     RETA"
     gene            5227..6153
                     /gene="argF"
                     /locus_tag="VFI55_14035"
     CDS             5227..6153
                     /gene="argF"
                     /locus_tag="VFI55_14035"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET7075186.1"
                     /translation="MTRHFLRDDDLSPQEQADVLQLAAELKKDPFSRRPLDGPRGVAV
                     IFDKNSTRTRFSFEVGIAQLGGHAVVVDSSNTQLGRSETLQDTAQVLSRYVEAIVWRT
                     FGQDRLDAMAAAATVPVVNALSDEFHPCQVLADLQTIAERKGSLRGLRLSYFGDGANN
                     MAHSLMLGGATAGIHVTVAAPKGFAPDPAVVAAAGQRAEATGASVALTDDADAAAEGA
                     DVLVTDTWTSMGQEDDGLDRVKPFRPFQVNSRMLGLADPEAIVLHCLPAHRGDEITDE
                     VMDGPASVVWDEAENRLHAQKALLVWLLERRL"
     gene            6150..6653
                     /locus_tag="VFI55_14040"
     CDS             6150..6653
                     /locus_tag="VFI55_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET7075187.1"
                     /translation="MTGGPKAAPEVSATRVGRQARIVSILSSSEVRSQSELAALLANE
                     GIDVTQATLSRDLEELGAVKLRGADGGTGVYIVPEDGSPVRGVAGGTDRMSRLLGELL
                     VSTDASGNLAVLRTPPGAAHYLASAIDRAALPQVVGTVAGDDTILVVAREPMTGAELA
                     DMFENIQ"
     gene            6666..>7253
                     /locus_tag="VFI55_14045"
     CDS             6666..>7253
                     /locus_tag="VFI55_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HET7075188.1"
                     /translation="MSERGSERVILAYSGGLDTSVAISWIGKETGREVVAVAIDLGQG
                     GEDMEVVRQRALDCGAVEAIVVDARDEFAEGYCLPTVLNNALYMDRYPLVSAISRPLI
                     VKHLVAAARAHGGGIVAHGCTGKGNDQVRFEVGFASLAPDLEVLAPVRDYAWTREKAI
                     AFAEENAIPINVTKRSPFSIDQNVWGRAVETGFLEH"
BASE COUNT         1128 a         2483 c         2521 g         1121 t
ORIGIN      
        1 caacctcggc gtggcgctgt cgggtctggg cggttcgttg agcactgcgc tgtcgttggg
       61 cctgagtggt tcgctttcgg gcctgggtcc gttgagtgcg ctgctcgagg gtggtctgac
      121 gggtgggttg tcgggtctgg ccaccaccgg tatctcgctg gccaacgcct tcggcgccgg
      181 gctcaacggg ctggttcaga ccggagcgtc cctggtggcc aacgtcggcg ccgagttggc
      241 caacctgaac ctgggcctgt cgatcaatgc cgccctgtcg gccagtctga acgcggccct
      301 gtcgggcctg gggcagaccg tgcaagcctt gacagccggc ctcggtggcg gcctgggcgg
      361 gctgattcac actggcgaag ccttgggcgg cagcctggcc gcaagcctgt ccgacctggg
      421 ggcgaccggt aacgcgctgg tgcgggtctg ggaggacgcc ggcgccgaga ttggttccgc
      481 gctcagcggg gctttgtcgg gaagcattgg cgttgccttc cctggcttta ttggggccgg
      541 tgccgccctg gcggccggtg ttgccggtgc cagcgctgga ctcgaacaga ccggcggctc
      601 actcgtgacg tctttggacg ttgcgttgac cgacctcgaa ctctcgctcc aggccgcgtt
      661 gagtgcgagc ctgggcgtcg gggtcgccgc ctaggggggt ggtgcccagg acgcccgcgt
      721 aggcgcgtgg ctcaccccaa gcggagccgg caccgaaacg gcgtcctggg cgcccggcgg
      781 ggcccccggc gaagcctggg aatggatttg caaactgctg cataaccatg cagaattgtc
      841 ggaatggccg acctattgcg cgtggcggtg gccggtgcca ccggctacgc gggcggcgaa
      901 atccttcgcc tactcctcgg acaccccgcc tgcgccgacg gccggctcac gatcggcgcg
      961 gtcacggccg cggccagtgc cggcagcacg ctcggagagc accatccgca cctaattccc
     1021 ttggcccagc gcgtggttga acccaccgaa cttgccgtgc tcgccggaca cgacgtggtg
     1081 ttcctcgcct tgccgcacgg acattcggcc gcgctggctg aacagctcag ccccgaaacc
     1141 ctgatcgtcg actgcggcgc cgacttccgg ctgaccgacg ccgctacctg ggaacggttc
     1201 tacggatccc cgcacgccgg cagctggccc tacgggttgc ctgaactgcc cggcggacgg
     1261 gagcggcttc gcggagcccg ccgtatcgcg gttcccggtt gctacccgac cgccgcgcta
     1321 ctggcgttgg cccccgcggt ggccggggac ctcatcgagc ccgccgtgac ggtggtcgcc
     1381 gtcagcggta cgtctggagc cggccgcgcg gccaaggccg acttgctcgg ctcggaggtc
     1441 atcgggtcgg cgcgggccta cagcatcgcg ggagcccacc ggcacacccc cgagatcgct
     1501 cagggactcc gcgccgtcac cgaccgcgac gtcacggttt cgttcacgcc ggtgctcatc
     1561 ccgacgtctc gcggcatcct ggccacctgc accgcccgca cccgatcacc gctgtcgcag
     1621 ctgcgcgctg cttacgaaaa ggcgtatgac gcagagccat tcatctatct tatgcccgaa
     1681 gggcaactgc cccgcaccgg agcggtgacc ggcagcaacg cggcgcacat cgccgtcgcg
     1741 gtggacgagg ccgccgagac gttcgtggcg atcgccgcga tcgacaactt ggtcaagggc
     1801 accggcggcg cggcggtgca gtcgatgaac ctcgcgctgg gttggccgga gacagaaggt
     1861 ctttcggtga tcggggttgc gccgtgactg aaatagctac tgccgcaagg ttgttgcgtg
     1921 agcaaggcgt caccgcgccg gccgggttcc gggccgcggg catcgccgcg gggatcaagg
     1981 catccggggc ccgcgacctc gcgctggttt tcaacgaggg ccccgactac gcggccgccg
     2041 gagtgttcac ccgcaaccag gtcaaagccg cgccggtgct gtggacccgg caggtgctga
     2101 ccaccgggcg gctgcgtgcc gtgatcctca actccggcgg tgccaacgcc tgcaccggac
     2161 cggccggttt ccaggacacc catgccacgg ccgaggtggt cgcggctgcg ctgtccgact
     2221 gggggaccga gaccggagcc atcgaggtcg cggtctgctc caccgggttg atcggtgacc
     2281 ggctcccgat ggacaaagtg cttaccggcg tgcgtgagat cgtgcgcgag atggccggcg
     2341 gcctgaacgg cggcgaggac gccgcccacg ccatcatgac caccgacacc gtgcccaaac
     2401 aggttgcgct gcaccatctg gacaactgga cggtcggcgg gatggccaag ggcgcgggca
     2461 tgctggcacc gtcgttggcc accatgctct gcgtgctcac caccgacgcc gccgtcgagc
     2521 cggcggcgct cgatcgggcg ttgcgccgcg ccagcgcccg caccttcgac cggctcgaca
     2581 tcgacggcag ctgttccacc aacgacacgg tgctgttgct agcgtccgga gccagtggga
     2641 tcaccccgcc tcaggccgat ctcgacgacg cggtgctgcg ggtgtgcgac gacctgtgcg
     2701 cccagctgca agccgacgcc gagggggtga ccaagcgggt cagcgtgacg gtcatggggg
     2761 ccgccagcga cgacgacgcg ctcaccgccg cccgggtgat cgcccgcgac agtctggtca
     2821 agactgcggt gttcgggtcc gaccccaact ggggccgcgt actcgcggcc gtcggcatgg
     2881 cgccgataac cctcgaccct gatcgaatca cggtgtcgtt caatggttct gcggtatgcg
     2941 tcgacggagc cggagccccg ggcgcccgcg aggtggacct ttccggggcc gacatcgaca
     3001 tcaacgtcga cctcggcgtc ggcgagggtc gggccaccat ccggaccacc gacctgtcgc
     3061 acgcctacgt cgaagagaat tcggcgtaca gctcgtgaac accaaaaccg aggcgctacc
     3121 cacgcaggtc aaagcgcagg tgctcgccga agccctgccc tggctcaagc agttgcacgg
     3181 caagatcgtc gtcgtcaaat atggcggcaa cgcgatgacc gacgacgcgc tgcgccacgc
     3241 cttcgcggcc gacatggcgt ttctgcgcaa ttgcggcatc catcccgtcg tcgtgcacgg
     3301 cggtggacca cagatcaccg cgatgctgcg gcggctcggc attgaaggcg acttcaaggg
     3361 cggcttccgg gtcaccacac cggaagtgct cgacgtggcg cggatggtgt tgttcggaca
     3421 ggtgggccgc gagctggtca acctgatcaa cgcgcacggg ccctacgcgg tcggtatcac
     3481 cggcgaggac gcacaacttt tcaccgcagt gcgtcgcagc gtcaccgtcg acggcgtggc
     3541 caccgacatc ggtctggtcg gcgacgtcga gctcgtcaac accgcggcgg tgctggatct
     3601 gattgcggca gggcgtattc cggtggtgtc tacgctcgcg ccggacgccg agggcgtggt
     3661 acacaacatc aacgccgaca ccgccgcggc ggcgctggcc gaagccctgg gagccgaaaa
     3721 gctgttgatg ctcaccgatg tcgagggcgt ctacgccagc tggccggacc gggactcgtt
     3781 ggtcagcgaa atcgacaccg ccacactggc gcaactgcta ccgacaatgg aggcgggcat
     3841 gatccccaag gtggaggcct gcctcagagc ggtcaccggg ggagtgccca gcgcgcacgt
     3901 catcgacgga agggtcgaac actgcgtgtt ggtggagctg ttcaccgacg cgggcaccgg
     3961 caccaaggtg gtgagcgtgt gaccgcgcgg gcgacgatgc agtgggggta ccgcccgctt
     4021 gcgggggaga gcggcgccat tgactaacac ggacaccatg aagcaccggt gggaatcggt
     4081 gatgatgaac aactacggca ccccgcccgt ggccctggcc agcggtgacg gcgccgtggt
     4141 caccgacgtg gacggcaaga cctacctgga cctgcttggc ggcatcgcgg tcaatgtcct
     4201 gggccatcgc caccccgccg tcatcgacgc cgtcacgcag cagatgtcga cgctgggcca
     4261 cacttccaac ttgtatgcca ccgaacccgg gatcgcactg gccgaggaat tggtcgccct
     4321 gttgggcacc gaccgtcaga cgcgagtgtt cttctgcaat tcgggcaccg aagccaacga
     4381 gctcgcattc aagctatcgc gtctcaccgg tcgcacgaaa cttgtcgccg cacaagaggc
     4441 tttccacggc cggacgatgg ggtcgctggc gctgaccggc cagccgacaa agcaggcgcc
     4501 gtttgagccg ctgccgggtg acgtcacgca cgtgccctac ggcgacaccg aggcgctggc
     4561 tgccgcggtc gacgacgaca cggccgccgt gttcctcgag ccgatcatgg gggagagcgg
     4621 cgtcgtcgtg ccgcccgagg gctatctggc caccgtgcgg gacattacgg cgcggcacgg
     4681 caccctgctg gtgctcgacg aggtgcagac cggaatgggc cgcaccggcg cctttttcgc
     4741 ccaccagcac gacggcatca cccccgacgt ggtgaccatg gccaaggggc tcggcggcgg
     4801 gctgccgatc ggcgcgtgcc tggccgtcgg gcccgccgcc gagctgctga caccaggcct
     4861 gcacggcagc accttcggcg gcaacccgat ctgcgccgcg gcggccctgg cggtgttgcg
     4921 ggtgctggcc gccgaagacc tggtccggca cgccgaggtg ctgggcaaat cggtgcgcca
     4981 cggcatcgag acgctcggac acccgctgat cgaccacgtg cgcggccggg gattgctctg
     5041 gggtgtggtg ctgaccgcgc cgctggccaa ggacaccgag gccgccgcgc gcgacgccgg
     5101 attcctggtc aacgccgccg cacccgacgt catccggttg gcgccgccgc tgatcatcac
     5161 cgacacccag atcgacggct tcatcgccgc cctcccgggc atcctcgacg cggcccggga
     5221 gacagcatga cgcgccactt cctgcgcgac gacgacctct cgccgcaaga acaggccgac
     5281 gtcctacagc tggccgccga gctgaagaag gacccattca gccgccgccc cctcgacggg
     5341 ccgcgcggcg tcgcggtcat cttcgacaag aactccaccc gcacccgatt ttcgttcgag
     5401 gtgggtatcg cgcagctcgg cggtcatgcg gtcgtggtcg acagcagcaa cacccagctc
     5461 ggccgcagcg aaaccttgca ggacaccgcg caggtgttgt cccgctacgt cgaggccatc
     5521 gtctggcgga ccttcggcca ggaccggctc gacgccatgg ccgctgccgc gacggttccg
     5581 gtggtcaacg cgctgtccga cgagtttcat ccgtgtcagg tgctcgctga tctgcagacc
     5641 atcgccgaac gcaaggggtc gctgcgcggc ttgcggctgt cctacttcgg tgacggcgcc
     5701 aacaacatgg cgcactcgtt gatgctcggc ggggccaccg ccggcatcca cgtcaccgtc
     5761 gccgccccca agggcttcgc gccggacccg gcggtggtcg ccgcggccgg tcaacgcgcc
     5821 gaagccaccg gcgcctcggt ggccctgacc gacgatgccg acgcggcagc cgagggcgct
     5881 gacgtcctgg tgaccgacac ctggacctcg atgggccagg aggacgacgg gctggaccgg
     5941 gttaagccgt tccggccgtt tcaggtcaac tccagaatgc tggggctggc tgatccggaa
     6001 gccattgtgc tgcactgcct tccggctcat cgtggcgacg agatcaccga cgaggtgatg
     6061 gacggaccgg ccagcgtggt ctgggacgag gcggagaacc gattgcacgc gcaaaaggcg
     6121 ctgctggtgt ggctgctgga gcggcgctta tgacgggcgg cccgaaggcg gcgcccgagg
     6181 tcagcgccac ccgtgtcggc cggcaggccc gcatcgtgtc catcctgtcg tcgtccgagg
     6241 tgcgtagcca aagcgaattg gcggcgttgc tggccaacga gggcatcgac gtcacccagg
     6301 ccacgctgtc gcgcgatttg gaagaactcg gcgcggtgaa gctgcgcggc gccgacggcg
     6361 gcaccggcgt ctacatcgtg cccgaggacg gcagccccgt ccgcggcgtc gcgggcggaa
     6421 ccgaccggat gtcgcgcctg ctcggcgagt tgctcgtatc gaccgacgcg agcggtaacc
     6481 tcgcggtgtt gcgcacgccg cccggcgcgg cgcactacct ggccagcgcc atcgaccgcg
     6541 cggccctgcc ccaggtggtc ggcaccgtcg ccggcgatga caccatcctt gtggttgccc
     6601 gcgagccgat gaccggcgca gaactcgcgg acatgttcga gaacattcag taaggagatt
     6661 ggtccatgtc agaacgcggg tcagaacgcg tcatcctggc gtattccggc ggtctggaca
     6721 cctcggtggc gatcagctgg atcggcaagg agaccggccg cgaggtcgtg gcggtggcga
     6781 ttgacctcgg ccagggcggc gaggacatgg aggtggtccg ccagcgggcc ctggattgcg
     6841 gcgccgtgga ggcgatcgtg gtcgacgccc gcgacgagtt cgccgaaggc tactgcctgc
     6901 cgacggtgct gaacaacgcg ctgtacatgg atcgctaccc cctggtgtcc gcgatcagcc
     6961 gacccttgat cgtcaagcac ctggtcgccg ccgcccgcgc gcacggcggc ggcatcgtcg
     7021 cgcacggctg caccggcaag ggcaacgatc aggtccggtt cgaggtcgga ttcgcctcgc
     7081 tggcaccaga tctcgaggtg ttggccccgg tgcgcgacta cgcgtggact cgggaaaagg
     7141 ccatcgcgtt cgccgaggag aacgccatcc cgatcaacgt caccaagcgc tcaccgttct
     7201 cgatcgacca gaacgtgtgg ggcagggcgg tggaaaccgg cttcctcgag cat
//
LOCUS       DASSGQ010000321         8652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_935127,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000321 DASSGQ010000000
VERSION     DASSGQ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935127"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1041)
                     /locus_tag="VFI55_14050"
     CDS             complement(172..1041)
                     /locus_tag="VFI55_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET7075189.1"
                     /translation="MAGRRALVLAGGGIAGIAWETGVLRGIADESPAASRLLLNSDVL
                     VGTSAGAAVAAQLGSGATLEALFDRQVAESSAEIDSGVDVDDITELFLAALGEPEDPT
                     TDRTRQQIQRIGAVALAAKTVPEQVRRQVIAQRLPSHDWPDRALRLTAIDVATGELVV
                     FDRETGVGLIDAVGASCAVPGVWPPVTVADRRYMDGGIASSVNLPVAGDCQVAVVLVP
                     SGADTPSPFGDGPAAEIASFTGATLAVFADDASLAAFGPNPLDPRCRVASAVAGREQG
                     RREAGAVARFLGV"
     gene            complement(1114..1992)
                     /locus_tag="VFI55_14055"
     CDS             complement(1114..1992)
                     /locus_tag="VFI55_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HET7075190.1"
                     /translation="MCRLFGVHAGTNACTATFWLLNAPDSLARQSRKNPDGTGLGVFD
                     EHGRPHVYKQPIAAWQDTRFATEAHRLTGTTFVAHVRYATTGSLDVRNTHPFLQDGRI
                     FAHNGVVQGLPDIDERLRELGTDDLVLGQTDSERVFALITGSIRAHDGDVTIGLVDAM
                     RWLAAKVPIYAVNILLSTATDMWGLRYPETHELYVLDRRPGHHTAEPDPEFDMRTKRI
                     RAQSEHLCTRSSVVFASEPMDDDAAWRLLEPGELVHVNAGLQITRSLVLPDPPAHPLR
                     QADLSTPVQQAQHTSV"
     gene            complement(2061..3701)
                     /locus_tag="VFI55_14060"
     CDS             complement(2061..3701)
                     /locus_tag="VFI55_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HET7075191.1"
                     /translation="MDSTMQDFPLTIAAIMRHGCGVHGMRTVTTATGSGYRQTSYHEL
                     GQQAAQLANALRGLGITGDQRVATFMWNNTEHLAAYLAVPSMGAVLHTLNIRLFPEQI
                     AYVANEAEDQIVLLDASLVKVLAPVLAQLDTVHTVIVVGDCPEGQAGPLQDSGKTVLQ
                     YAEVIGGESTEFDWPQIDERSAAAMCYTSGTTGNPKGVVYSHRSTFLHSLAVCTSNGI
                     GVGASDKVLPIVPMFHANAWGLPYAALVAGADIVLPDCHLDGRSLIDMIEKLRPTVAG
                     AVPTIWNDVMHRLEDDPDHDLTSLRMVACGGSAVPVSLMRGFEEKHGVQIRQLWGMTE
                     TSPIATMAWPPPDAPEDQHWAFRGTAGQPLCGVEARIVNDDGRVLPNDGKTVGELEVR
                     GPWITGSYYLGRDASKFESGWLRTGDVGRLDGRSFITLTDRAKDVIKSGGEWISSVEL
                     ENWLIAHPDVVEATVVGVPDERWQERPLAVVVAKPGASVTAQELRTFLADKVARWWLP
                     ERWTFVDEVPRTSVGKYDKKAIRSRYAEHAYSVVEARD"
     gene            complement(3838..4995)
                     /locus_tag="VFI55_14065"
     CDS             complement(3838..4995)
                     /locus_tag="VFI55_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HET7075192.1"
                     /translation="MSDVKHREQLPDGQVIELGAAPGFPIVVHVAVHNGPISGMAASP
                     DGNRLMVANYGSDSVSVIDVDTCRVVATVAGLDEPFAIAMGGRDSDRAYVSTVSTAYD
                     SIAVIDVPTNTVVATHPLALSVSDLAVSPDGKYVYAARHDAGGADLAVLDSTTGQLEA
                     IDLPAQAGGTTECVRVSSDGGRVCVATNGPAGGRLVVLRTPARSDGRGGRSKGWRRKS
                     AARSRAVETNKTIEIGLPIRDVALSPDSSSAYVASCAPDGGVVIDVVDLRTAKITATR
                     KIGEIGGILTGLTLSGDGDRAYLVSDDSITVLCTLTHDIIGTVGVADQPSCVVESPNA
                     KFLYIADYSGAVTVVPVASIVALGVATPALESSPPEEWVVPELLGCQAALA"
     gene            complement(5455..5793)
                     /locus_tag="VFI55_14070"
     CDS             complement(5455..5793)
                     /locus_tag="VFI55_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET7075193.1"
                     /translation="MKLVTAIVKPFTLDDVRHAVEAVGVLGLTVTEVQGYGRQRGHTE
                     VYRGAEYAVEFVPKVRVEVLVDAEVADRVVDAIIGAARTGKIGDGKVWVSPVEKVIRI
                     RTGERDHEAL"
     gene            complement(5809..7224)
                     /locus_tag="VFI55_14075"
     CDS             complement(5809..7224)
                     /locus_tag="VFI55_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET7075194.1"
                     /translation="MLPYPDWLNPGDNAWQLVAATLVGLMSIPGIAVLYGGIVQKKWA
                     VNTMLMAFTGFSLVLVVWVLWGFKMGFGEPLKLGPGILQAAIGKPRTILSSNNEQIAW
                     IPLLDGAMPSFRFSETTLAYFQFVFAAITPLLFLGSVIGRISFKVWLIFVPLWSTFAY
                     SVNAFLLWGGGWWAQGSHAALDYSGGYVIHLAAGTTGFVAAAVIGPRLQRDRERAVPN
                     NLPLAAVGAGILWLGWNGFNGGDPYFAGADASLAVLNTNLATACALLTWVVWDLFVSK
                     QRKPTFLGAVNGMITGLVAITPAAGFVNSFGAMIIGVVASSLVWMSWNWLGTTKLFKK
                     VDDTLGVFHTHGVAGLAGGLLVGVLADPHVVEYLGGTSGADVTAAGWLYGNHPKQILI
                     QAGAAGTVIVWDALVTFVILKVLSLFMKLRLPDEVLETGDVGVHDEEAYPDDTLVTGR
                     REAFATTRADSAAKSAESVDD"
     gene            complement(7380..8171)
                     /locus_tag="VFI55_14080"
     CDS             complement(7380..8171)
                     /locus_tag="VFI55_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HET7075195.1"
                     /translation="MTNDIGDRNYPQMAASRGRIEPAPRRVRGYLGHELLFDTTAARY
                     VWELPYYPAYYVPLADVRQEFLSDENHPQKVQFGPSRLHSLVGGGQTYPSAARVFDAD
                     GDGPLAGTVRFEWDPLRWFEEDEPIYGHPRNPYSRVDALRSHRHVRVELDGIVLADTK
                     SPVLLFETGLPTRYYIDPTDVSFEHLEPSPTQTLCPYKGVTSGYWSVRVGSAAEQTVH
                     PDLAWTYHYPLPAVGQIAGLVAFYNEKLDIVVDGAALPRPRTQFS"
     gene            complement(8179..8451)
                     /locus_tag="VFI55_14085"
     CDS             complement(8179..8451)
                     /locus_tag="VFI55_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6295 family protein"
                     /protein_id="HET7075196.1"
                     /translation="MCTHLTEYVQIDGSGKGATGWFGVDRATVYVDHPVHAPYAHTVN
                     IDVLNPRLGPSARVALELTEESALALADAIRKALSHAPAGLASKNQ"
     gene            complement(8460..>8652)
                     /locus_tag="VFI55_14090"
     CDS             complement(8460..>8652)
                     /locus_tag="VFI55_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET7075197.1"
                     /translation="FPAPSAVAALDAELTRLDKPHEFHSYEGAGHAFFSVDRPVYRVT
                     AAVDGWLRIDEFFATHLKG"
BASE COUNT         1465 a         2948 c         2819 g         1420 t
ORIGIN      
        1 cctgacgctg gcgcgccagg cggcagtgat caccgccgca cgcgcggcct ccgacgacga
       61 atgcgggtag cgccggggcg gcgatccggg acccccgcgc aattgagatc acgggtgaaa
      121 aaactgctgc gacgcccgcg atcgagggtt gatcgacacc cgagcgctgc gtcagacacc
      181 caggaagcga gccacggccc cggcttcgcg gcggccctgt tcccgcccgg ccaccgccga
      241 agcgacccgg cagcggggat ccagcggatt gggtccgaac gccgccaacg aagcgtcgtc
      301 ggcgaacacc gcgagggtcg cgccggtgaa cgacgcgatc tcggcggcgg gtccatcgcc
      361 gaacggtgac ggcgtgtcgg cgcccgaggg caccagcacc acggccacct ggcaatcgcc
      421 ggccaccgga aggttgaccg agctggcaat cccgccgtcc atgtagcgcc ggtccgcgac
      481 cgtcaccggt ggccacactc ccggcaccgc gcaactggcc ccgaccgcgt cgatgagccc
      541 gacgccggtc tcccgatcga aaacgaccaa ttcgccggtt gcgacgtcga tcgcggtcag
      601 ccgcagggcc cggtccggcc agtcatgcga tggcaggcgc tgcgcaatca cctggcgtcg
      661 gacctgctcg gggaccgtct tggccgccaa cgccaccgcc ccgatccgct gtatctgctg
      721 tcgggtcctg tccgtggtcg ggtcttcggg ttcgcccaag gcggccagga acagttcggt
      781 gatgtcgtcc acgtcgacgc cggaatcgat ttccgccgac gactccgcga cttgccggtc
      841 gaacagcgcc tcgagcgtgg cgccgctgcc gagctgcgcg gcgaccgccg cccctgccga
      901 ggtacccacc agcacatcgg aattcagcag cagccgcgac gccgccggcg actcgtcggc
      961 gatgccgcgc agaacacctg tctcccaggc gattccggcg atgccgccgc cggccagcac
     1021 gagcgcacgt ctgcctgcca cgtccactca ctctgccagc accgggcggg ggcgccgcaa
     1081 tagccctcag accgcccaga ccgctcagac cgcttacacc gaagtgtgct gggcctgctg
     1141 gaccggcgtg ctcaggtcgg cctggcggag cggatgcgcg ggcgggtcgg gcagcaccag
     1201 actgcgggtg atctgcagcc cggcgttcac gtggaccagc tcgcccggct cgagcagacg
     1261 ccaggccgcg tcgtcgtcca tcggttcgct ggcgaacacc accgacgagc gcgtgcacag
     1321 gtgctccgac tgcgcgcgaa tccgcttggt gcgcatgtcg aattcgggat cgggctcggc
     1381 cgtgtgatgg ccggggcggc ggtccagcac atagagctca tgggtttccg gatagcgcag
     1441 cccccacatg tccgtggccg tgctcagcag gatgttgacc gcgtaaatgg gcaccttcgc
     1501 cgcaagccac ctcatggcgt cgaccagacc gatcgtcacg tcaccgtcgt gggcccggat
     1561 cgacccggtg atcagcgcga acacccgctc ggaatcggtc tggcccagga ccaggtcgtc
     1621 ggtgccgagt tcgcgtaacc gttcgtcgat gtcgggcagc ccctggacca cgccgttgtg
     1681 cgcaaagatt cggccgtcct gcaaaaacgg gtgggtgttg cgcacgtcga gcgacccggt
     1741 cgtcgcatag cgaacatggg cgacgaaggt cgtgcccgtc agccggtgcg cttcggtggc
     1801 gaagcgggtg tcctgccagg ccgcaatcgg ctgcttgtac acgtgcggac gcccgtgctc
     1861 gtcgaaaaca cccaggcccg tgccgtcggg gttcttccgg ctttgccggg ccaggctgtc
     1921 tggagcattg agcagccaaa acgtcgcggt gcatgcgttg gtcccggcgt gcacgccaaa
     1981 gagtcggcac atgatttcga cggtaccgaa agccgcgccg acgtcaggtc cccctcagcg
     2041 cgccagcggt tgggcggcgc tcagtcgcga gcctcgacga ccgaataggc atgctcggcg
     2101 tatcgcgacc ggatggcctt cttgtcgtac ttgcccacgc tggtgcgcgg aacctcatcg
     2161 acgaacgtcc accgctcggg cagccaccac cgagcgacct tgtccgccag aaacgttcgc
     2221 aactcctggg ccgtgaccga cgcgcccggt ttagccacga cgacggcgag cggtcgttcc
     2281 tgccatcgct cgtcgggaac ccccacgacc gtggcctcga ccacgtcggg gtgggcgatc
     2341 agccagttct ccaactcgac cgacgagatc cattccccgc ccgacttgat gacgtccttg
     2401 gcccggtcgg tcagggtgat gaagcttcgc ccgtcgaggc ggccgacgtc gccggtgcgc
     2461 aaccagcccg actcgaactt ggaggcatcg cgccccaagt agtaggagcc ggtgatccac
     2521 gggccgcgga cttccagttc ccccacggtc ttgccgtcat tgggcagcac ccggccgtcg
     2581 tcgttgacga tccgggcttc cacgccacac agcggttgac ccgcggtgcc gcggaatgcc
     2641 cagtgctggt cctccggggc atccggtggc ggccaggcca tggtggcgat cggagacgtt
     2701 tccgtcatgc cccacagctg ccggatctgg acgccgtgct tttcctcgaa gccgcgcatc
     2761 agcgataccg gaaccgccga gccaccgcag gccaccatgc gcagcgacgt caagtcgtgg
     2821 tccgggtcgt cctcgaggcg atgcatgacg tcgttccaga tggtcggcac ggcgcccgcc
     2881 accgtgggcc gcagcttttc gatcatgtcg atcagcgatc gcccgtcaag atggcaatcg
     2941 gggagcacga tgtcggcccc ggccaccagc gccgcgtacg gcagccccca cgcgttggcg
     3001 tggaacatcg gcacgatcgg cagcaccttg tcgctggccc cgactccgat cccgttgctg
     3061 gtacataccg ccaggctgtg caggaaggtc gaacgatggc tgtagaccac gcctttgggg
     3121 ttgccggtgg tgccgctggt gtagcacatt gcggccgcgg acctttcgtc aatctgcggc
     3181 cagtcgaatt cggtcgactc gccgccgatc acctcggcat actgcagcac tgtcttaccc
     3241 gaatcctgca gcggacccgc ctggccttcc gggcaatccc cgacgacgat caccgtgtgc
     3301 acggtgtcga gctgggccag taccggcgcc agcaccttga ccagtgacgc gtcgagcagc
     3361 acgatctggt cctcggcttc gttggcgacg taggcgatct gctcgggaaa cagccggatg
     3421 ttgagcgtgt gcagaaccgc gcccatcgaa ggcaccgcga ggtaggccgc caggtgctcg
     3481 gtgttgttcc acatgaacgt cgccacccgc tggtcgccgg taatgccgag gccgcgcaat
     3541 gcgtttgcca gctgggccgc ttgctgaccc agctcgtggt agctggtctg gcggtaaccg
     3601 cttccggtgg cggtggtgac ggtccgcatt ccgtggacac cgcagccgtg ccgcatgatc
     3661 gcggcgatcg tcagcgggaa gtcctgcatc gtgctgtcca tcgaggtacc gccttagggt
     3721 gttagcgcag caccgcctca tgtgcggtcg ggcgcatcca ccctaagccc cagtccagcg
     3781 aacgcaataa ttgttcgcta cgggccttcg caacgggcca gacgccttgc cgaacggtta
     3841 ggcgagagcc gcctggcacc ccagaagctc gggcacgacc cactcctccg gcgggctgct
     3901 ctcgagggcc ggggtcgcga cgcccaaggc gacgatcgag gcgacgggca ccaccgtgac
     3961 ggcgcccgaa tagtcggcga tgtagaggaa cttcgcgttc gggctttcga ccacgcacga
     4021 cggctgatcc gccacgccga cggtgccgat gatgtcgtgt gtgagcgtgc acagcaccgt
     4081 gatgctgtcg tcgctgacca ggtaggcgcg atcgccgtcg ccgctcagcg tcagtccggt
     4141 gagaatgcca ccgatttcgc cgatcttgcg cgtggcggtg atttttgcgg tgcgcaggtc
     4201 gacgacgtcg atcaccaccc ccccgtcggg agcgcaactg gccacgtacg cgctcgacga
     4261 gtcggggctg agcgcgacgt cgcggatggg cagaccgatt tcgatcgtct tattcgtctc
     4321 gacggcgcgc gaccgggcgg cgctcttgcg gcgccagcct ttggatcgcc cgccacgacc
     4381 gtcggatcgc gccggtgtcc ggagcacgac gagccgccca ccggccgggc cgttggtggc
     4441 cacgcacacg cggcccccgt cggaggagac gcgcacgcat tccgtagtgc cgcccgcctg
     4501 ggcgggaagg tcgatggcct ccagctggcc ggtcgtgctg tccagcacgg cgaggtcggc
     4561 cccgccggcg tcgtgccggg cggcatagac gtactttccg tccgggctta cggccaggtc
     4621 gctcacgctg agcgccagcg gatgcgtcgc gacgaccgtg ttggtcggca cgtcgatgac
     4681 cgcgatcgag tcatacgccg tcgacacggt gctgacgtag gcgcggtcgc tgtcgcgacc
     4741 gcccatcgcg atcgcgaacg gctcgtcgag gccggccacg gtcgccacga cccggcaggt
     4801 gtcgacgtcg atcaccgaga cgctgtcgct gccgtagttg gccaccatca atcggttgcc
     4861 gtcggggctg gcggccatgc cgctgatcgg accgttgtgc acggcaacgt ggacgacgat
     4921 cgggaatccc ggcgcggcgc ccaactcaat gacctggcca tccgggagct gctcccggtg
     4981 cttaacatcg ctcatgctga caccgccttt gctgcgttcg tggtacgtcc aaggggctgg
     5041 atcgcggact acgctgtgcg aaaacactgc cccggattca ccggactccg gcacctaata
     5101 ggtgactgct tgagataaat tttaccggcc gaattctgcc caaattaaat tcagcaagcc
     5161 ggcactgttg aacgcgtcac acgatggccc gggccgcgct tagactttcg ttatccagca
     5221 caccaagacc cagtgaacgg caaacagccg ctgcatcgtt gacaagcatg atgcgaactt
     5281 tgtggctaac ccgcggcacc cgcgacgccc gggaccggta aataacaaac tcttagaatt
     5341 tgccgccaaa aaagaggcag acgtcacagt aacgggtttg cgggcgggtt ttgcgagcca
     5401 gctttgcgtg catcggtacc gcaacaattc ggtcgccgcg cgatgccgtt gccctcagag
     5461 cgcttcgtgg tcgcgttccc cggtccggat gcggatcacc ttttcgaccg gcgataccca
     5521 caccttgccg tcgccgatct tcccggtgcg ggccgcgccg ataatcgcgt cgacaacgcg
     5581 gtcggccacc tccgcatcga cgagcacctc gacgcggacc ttcggaacga attcgaccgc
     5641 gtactcggct ccgcggtaga cctcggtgtg ccctcgctgc cggccgtaac cctgcacttc
     5701 ggtgacggtc aagcccagca cgccgacggc ctccacggcg tgcctgacgt catcgagcgt
     5761 gaacggcttg acgatcgcgg taaccagctt catggcctgc tcacagcctc aatcgtccac
     5821 tgactcggcg gatttggccg cggaatcagc ccgcgttgtg gcaaacgcct cccgccggcc
     5881 cgtgacgagc gtgtcgtccg ggtacgcctc ttcgtcgtgc acgccgacgt cgccggtttc
     5941 cagcacttcg tcgggcagcc gcaacttcat gaacaggctg aggactttca agatgacaaa
     6001 cgtgacgagg gcatcccaga cgatgaccgt gcccgcggcg ccggcctgga tcaatatctg
     6061 tttgggatgg ttcccgtaca accaaccggc ggcggtcacg tccgccccgc tggtgccgcc
     6121 caggtattcg acgacgtgcg ggtccgcgag caccccgacg agcaacccgc ccgccagacc
     6181 cgctacgccg tgggtgtgga acacccccag ggtgtcgtcg accttcttga acagtttcgt
     6241 ggtgccgagc cagttccagg acatccagac cagggacgag gcgacaacgc cgatgatcat
     6301 cgcgccgaag ctgttgacga agccggccgc cggggtgatg gcgacaaggc cggtgatcat
     6361 gccgttgacg gcacccagga acgtgggctt gcgctgcttg ctgacgaaga ggtcccagac
     6421 cacccaggtg agcaacgcgc atgcggtcgc gaggttggtg ttgagtaccg ccagcgacgc
     6481 gtcggcaccg gcgaagtagg ggtcaccgcc gttgaaccca ttccagccca accacaggat
     6541 gcccgcgccg accgcggcaa gcggcaggtt gttcggcacc gcgcgttccc ggtcccgctg
     6601 cagtcgcgga ccgatcaccg cggcggcgac aaacccggtc gttccggccg ccaggtgaat
     6661 cacgtagcca ccggagtagt ccagagcggc gtgcgagcct tgcgcccacc agccgccgcc
     6721 ccacaacagg aacgcgttga ccgagtaggc gaacgtcgac cacagcggca cgaagatcag
     6781 ccacaccttg aagctgatcc ggccgatcac gctgccgagg aacagcagcg gcgtgatggc
     6841 cgcgaacacg aactggaagt aggccaacgt ggtctccgag aaccggaaag acggcatggc
     6901 gccgtctagc agcgggatcc acgcgatctg ctcgttgttg ctgctcagga tcgtcctggg
     6961 tttcccgatc gccgcctgca ggatgccggg gccgagcttc agcggctcgc cgaagcccat
     7021 cttgaaaccc cacagcaccc atacgaccag caccagcgaa aacccggtga aggccatgag
     7081 catggtgttg acggcccact tcttctgcac gatgccgccg tacagaacag cgatacccgg
     7141 aatgctcatc aggccgacca gagtcgccgc caccaactgc caggcgttgt ctccgggatt
     7201 tagccaatcg ggatagggca acatagttca gacacacccc aattcgagcc ggcaggctgg
     7261 taattcagct gcggtggtag tgacgccgac aacgggtgaa gcctcgaacc ggaatgtttc
     7321 gggcgcatgt tcgccgcttt aaatattgat ttcgttcgac ggccccgtga cgcctcgaat
     7381 tagctgaatt gcgtccgcgg ccgtggcagg gcggccccgt cgaccacgat gtcgagcttc
     7441 tcgttgtaga aggccaccag gccggcgatc tggccgaccg ccggcagcgg atagtggtag
     7501 gtccacgcca ggtccggatg caccgtttgc tcggccgcgg agcccacccg caccgaccag
     7561 tagccagacg tgacgccttt gtacggacac agcgtctgcg tcgggctggg ttccagatgc
     7621 tcgaaggaga catcggtcgg gtcgatgtaa taccttgtgg gcaagcctgt ttcgaacaac
     7681 agcacgggag acttcgtgtc ggccaacacg attccgtcga gctcgacgcg gacgtggcga
     7741 tgcgagcgca gcgcgtcgac gcgcgaatag ggattgcgag ggtggccgta gatgggctcg
     7801 tcttcctcga accaccgcaa cggatcccat tcgaatcgca cggtgcccgc cagcgggccg
     7861 tcaccgtcgg cgtcgaacac ccgcgcggcc gacggatagg tctggccgcc gccgaccagg
     7921 gagtgcagcc gcgacggacc gaactgcacc ttctgcgggt gattctcgtc gctcaggaac
     7981 tcctgcctga catcggctag cggcacgtaa tacgccgggt aatagggcaa ttcccacacg
     8041 taacgggccg cggtggtgtc gaagagcagc tcgtggccga gatagccacg gacccgccgg
     8101 ggcgcgggct cgatccgccc gcggctggcg gccatttgcg ggtagttgcg gtccccgatg
     8161 tcgttggtca tgtctcacct actggttctt ggaggccagc cccgccgggg catgggagag
     8221 cgctttgcgg atggcgtcag ccagcgccag cgcgctctct tcggtgagct cgagcgccac
     8281 ccgcgccgac gggccgagcc gcggattgag cacgtcgatg ttcaccgtgt gcgcgtaggg
     8341 cgcatggacg gggtggtcga cgtacacggt cgctcggtcg acgccgaacc atcccgtagc
     8401 gcctttgccg ctgccgtcga tctgcacgta ttcggtcaga tgtgtgcaca tgggcccgcc
     8461 tagcctttca gatgggtagc gaagaactcg tcgatgcgaa gccagccgtc caccgccgcc
     8521 gtcacccggt ataccggccg gtcgactgag aagaacgcgt ggcccgcgcc ctcgtaggag
     8581 tggaattcgt gtggcttgtc cagcctggtc agctcggcgt ccagcgcggc caccgcagac
     8641 ggcgccggga at
//
LOCUS       DASSGQ010000322         8480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1330457,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000322 DASSGQ010000000
VERSION     DASSGQ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8480
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330457"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="VFI55_14095"
     CDS             <1..1353
                     /locus_tag="VFI55_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinofuranosyltransferase"
                     /protein_id="HET7075198.1"
                     /translation="YSAMITVLLPPVLVLTWSGLRAGERAAGRTLDTEREAGRTAERR
                     GGWAAVVGAGIFLGFTATWYLLLLAFSAFTMSLMALLLAGLRWRRNGFTAALDPLRRL
                     ATIAVIAAPIAGTTWLPYLLRAAHSPVSNTGSAYHYLPADGAVLTFPMLQFSLLGAIC
                     MLGTLWLVLRAPTSVRASALAIGVLAVYLWSLLSMLTTLARATLLSFRLQPTLTVLLV
                     TAGAFGFLEATRALSAWGHDRDWGRGSQRSRAPVAGAVGLAAAIAFSQDIPDVLRPDL
                     TIAYTDTDGYGQRGDRRPPSSEKFYSVIDAAILKATGKPRDQTVVMTADYSFLSYYPY
                     WGFQGLTSHYANPLAQFDLRAAQIEKWSKLKTPDDFIHALDTSPWPPPTVFLMRRGPG
                     SGLGTSYTLRLAQDVYPNQPNVRRYTVDMRAALFADPRFVVESIGPFVLAVRKPVTGS
                     "
     gene            1353..4625
                     /locus_tag="VFI55_14100"
     CDS             1353..4625
                     /locus_tag="VFI55_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052636 - arabinosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071766 - Actinobacterium-type cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosyltransferase domain-containing protein"
                     /protein_id="HET7075199.1"
                     /translation="MASETSPDLVEQLPSSSVTDAERNYRVARLVATVAGLLGALLAV
                     VTPVLPVNQTTAQLNWPQNGTFESVEAPLIGYVATDLNVTVPCQAAAGLAGPKNPART
                     VLLSTVPKQAPKAVDRGLLILRANDDLVLVVRNVPVVSAPLSQVLSPACQRLTFTAHP
                     DRVVAEFVGLTQGPDSEHPGSPLRGEKSGYDFRPQIVGVFTDLSGPTPPDLSFTATVD
                     TRYSSSPTPLKLAAMILGVLLTAIALIALHILDTADGTRHRRFLPARWWSIGALDALV
                     IAVLVWWHFVGANTSDDGYILTMARVSEHAAYMANYYRWFGTPEAPFGWYYDLLALWA
                     HVSTTSVWMRLPTLAMALTCWWVISREVMPRLGHAVKKSRSAAWTAAGMFLAVWLPLD
                     NGLRPEPIIALGILLTWCSVERAVATSRLLPVAIACIIGALTLFSGPTGIASIGALLV
                     AIGPLRTILHRRSKRFGALPLLAPILAAATVTAILIFRDQTLAGEAQASTLKRAVGPS
                     LSWFDEHTRYERLFMASPDGSIARRFAVLALILALGITVAVSLRKGRIPGTAAGPSRR
                     IVGITIISFLAMMFTPTKWTHHFGVFAGLAGSLGALAAVAVTSVAMRSRRNRTVFAAA
                     VLFVVALSFASVNGWWYVSNFGVPWSNSFPKWHYAFSTILLALTALVLLLAAWFHFVF
                     NGDAPARDGRTGYRARVIAIIESPLAVATWVLVIFEVVSLTLAMTEQYPAWSVGRSNL
                     EALAGKSCGLAEDVMVEQDPNAGMLTPVSASVADALGAGLAEAFTPNGIPADVRADPV
                     MERPGDLSVVNEELLATGAEAGTEGGTNPAPGINGSVAQLPYYLDPARTPVLGSWRSG
                     VQVPARLRSGWYRLPTRDKAGPLLVVSAAGRFDPREVQVQWATDDEAASGHPAGSFQF
                     ADVGAAPAWRNLRLPLSAIPTTATQIRLVADDEDLAPQHWIALTPPRIPQLRTLQDVV
                     GSRDPVLLDWLVGLAFPCQRPFGHQNGVNETPKWRILPDRFGAEANSPVMDNNGGGPL
                     GVTELVVKATTVATYLKDDWSRDWGSLQRMTAYYPNAQPARLQLGTATRSGLWSPGPL
                     RH"
     gene            complement(4705..4926)
                     /locus_tag="VFI55_14105"
     CDS             complement(4705..4926)
                     /locus_tag="VFI55_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075200.1"
                     /translation="MNAKSKKVSKLRMAAAVGLGTLGLSVAGSLVPAVASADPWVPYS
                     GPLHPIRHYAADVLHPIWTLTHPIRSLVP"
     gene            complement(5085..5723)
                     /locus_tag="VFI55_14110"
     CDS             complement(5085..5723)
                     /locus_tag="VFI55_14110"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET7075201.1"
                     /translation="MSASDAWEQAKQAWRRIAARGGRPGDDGLEALSDIGVVRRMLDQ
                     AELAAVRTARRHGKSWAEIATHLGVTRQSAWERWRDLDDAGDQARGAESGVAEVAANL
                     VQMRLQERRTQKVTVPNVVGLEWLRARAVLNADGLVAINAEADFPTAPGDGGWIVTDQ
                     SPESGARVDAGSVIRLWLKGNGGAGVREPRRPRPTPRPAREMRPEPRDQAVG"
     gene            5815..6147
                     /locus_tag="VFI55_14115"
     CDS             5815..6147
                     /locus_tag="VFI55_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7075202.1"
                     /translation="MSTYRVAEDGDAWLALADGTRRAIVERLARGPSAVGDLARDLPV
                     SRPAVSQHLKVLKSAGLVRDRAAGRRRVYQLDPTGLEVLRADLDRFWAQALATYAQTI
                     DETKGETP"
     gene            6144..6626
                     /locus_tag="VFI55_14120"
     CDS             6144..6626
                     /locus_tag="VFI55_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7075203.1"
                     /translation="MTQRRSVTHHVVVNAPIERAFAVFTERFGDFKPREHNLLAVPIA
                     ETVFEPRVGGHIYDRGVDGSVCRWARILVYQPPRRVIFTWDIGPTWQRETDESKTSEV
                     EVRFTAESDERTRVDLEHRHLERHGVGWESVADAVDGDAGWPLYLDRYDGLLSTEAPK
                     "
     gene            6623..7246
                     /locus_tag="VFI55_14125"
     CDS             6623..7246
                     /locus_tag="VFI55_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HET7075204.1"
                     /translation="MTATLMTMAHDERADLAEFLATLTPRDWEAPSLCTKWSVKDVVA
                     HVISYEELGTLGLVKRFAKGWIVRTNQVGVDEFAALSPQQLLEFLRNHLQPHGLTAGF
                     GGMIALVDGTIHHQDIRRALGRPRTVPVDRLERVLGLVPGNPRLGAGRRIRGLRLRAT
                     DIDWAHGRGPEVTGPGEALLMAMSGRPAALADLRGPGLDTLARRLGG"
     gene            complement(7254..8387)
                     /locus_tag="VFI55_14130"
     CDS             complement(7254..8387)
                     /locus_tag="VFI55_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET7075205.1"
                     /translation="MTTTGLGFGALGPLLLTSHGVRVPAGAPKQRAVLAVLLINRNRA
                     VSVEALINAVWDEDPVPAARTSIQAHVSNLRRLLRDAGVDPYQVLASAPPGYQLSVAD
                     DDYDVGRFTAAKAAGTKAVAAGLFEDASRHLSAALAEWRGPVLDDLRDFGFVDPFATT
                     LMEEKLAAHTALAEAEIACGRPGALIGELEALTAAHPYHEPLWAQLITAYYVTERQSD
                     ALRAYGRLKTALADGLGIDPGPTVTALYERILRQEPLVGRRADLTTRKRLSVTLAPAA
                     PALAEAAVARLRDKSGRQYRLNGATTRLGRLDDNDIVLDGEDVSRHHAVIIDTGTGFV
                     ITDLRSTNGVEVQGQRIHSNATLSDGARIRIGGHAFTFEIRPG"
BASE COUNT         1248 a         2943 c         2870 g         1419 t
ORIGIN      
        1 tactcggcga tgatcacggt gttgctgccg ccggtgctgg tgctcacgtg gtcgggactg
       61 cgcgccggcg agcgtgcggc tggccgcacg ttggacaccg aacgtgaagc tggccgcaca
      121 gccgagcgca ggggcggctg ggcggcggtg gtcggcgccg ggatattcct cggcttcacc
      181 gccacctggt acttgctgtt gctggccttc agcgcgttca cgatgtccct gatggccctg
      241 ctgttggccg gcctgcgctg gaggcgcaac ggcttcacag ccgcgctcga cccgctgcgc
      301 cggctggcca ccatcgccgt gatcgccgct cccatcgccg gcaccacctg gctgccgtat
      361 ttgttgcgcg cggcccacag tccggtcagc aacaccggca gtgcctacca ctacctgccg
      421 gccgacgggg cggtgctgac cttcccgatg ctgcagttct cgctgctcgg agcgatctgc
      481 atgctgggca ccctgtggct ggtgttgcgg gcgcccacat cggtgcgagc gagcgccctg
      541 gccatcggcg tgctggccgt ctacctgtgg tcactgttgt cgatgctgac cacgctggcg
      601 cgcgccacgc tgctgtcgtt ccggctgcag ccgacgctga ccgtgctgct ggtgaccgcg
      661 ggtgcgttcg gcttcctcga ggccacccgc gcgctatcgg catggggcca cgaccgtgac
      721 tggggccggg gttcacaacg atcgagagcc ccggtggccg gggctgtcgg cctggccgcc
      781 gcgatcgcct tcagccagga cattcccgat gtactgcggc cggatctgac aatcgcctac
      841 acggacaccg acggctacgg gcagcgcggc gaccgccggc cgccgagctc ggagaagttc
      901 tactccgtca tcgacgccgc catcctcaag gcgacgggca aaccgcgtga ccagacggtg
      961 gtgatgaccg ccgactacag ctttctgtcc tactacccct actggggatt ccaggggctg
     1021 acgtcgcatt acgccaaccc gctggcgcag ttcgatttgc gggccgcaca gatcgagaag
     1081 tggtccaagc tcaagacccc cgacgacttc atccacgcgt tggacacgtc gccgtggcct
     1141 ccgccgacgg tgtttctgat gcgccgcggc cctggttctg gtttggggac cagctacacc
     1201 ttgcggttgg cccaggacgt gtacccgaat cagccgaacg ttcgccgcta caccgtggat
     1261 atgcgggcgg ccctgttcgc cgatccgcga ttcgtcgtgg aaagcatcgg cccgttcgtg
     1321 ctggccgttc ggaagcccgt gacaggcagc tgatggccag cgaaacctcg cctgacttgg
     1381 tcgaacaact accatctagc tccgtgaccg atgcggaacg caactaccgg gtcgctcggt
     1441 tggttgctac cgtcgcgggt ctgctcggag cgctgctcgc ggtcgtcacc cccgtgctgc
     1501 cggtcaacca gaccaccgcc cagctgaact ggccgcagaa cggcacgttc gagagcgtcg
     1561 aggcaccgct gatcgggtac gtggccaccg acctgaacgt caccgtcccg tgccaggcgg
     1621 ccgccggact ggccgggccc aagaaccccg ccaggacggt gctgttgtcg acggtgccca
     1681 agcaggcgcc caaggccgtc gaccgcgggc tgctcatcct gcgcgccaac gacgacctgg
     1741 tgctggtggt gcgcaacgtc ccggtggtca gcgccccgtt gagccaggtg ctcagcccgg
     1801 cctgccagcg gttgaccttc accgcgcacc ccgaccgggt ggtcgccgaa ttcgtcgggc
     1861 tcacgcaggg acccgactcc gagcatcccg gctcgccctt gcgtggggag aagagcgggt
     1921 acgacttccg cccgcagatc gtcggcgtgt tcaccgatct ttccggtccg accccgccgg
     1981 acctcagctt cacggcgacc gtcgacaccc gctacagcag cagccccaca ccgctgaagc
     2041 tcgccgcgat gatcctcggg gtgctgctga ccgcgatcgc cctcatcgcg ctgcacatcc
     2101 tcgacaccgc cgacggcacc cggcaccggc gctttctgcc cgcgcgttgg tggtcgatcg
     2161 gcgccctgga cgcgctggtc atcgccgtgt tggtgtggtg gcatttcgtc ggcgccaaca
     2221 cctccgacga tggctacatc ctgaccatgg cccgggtgtc cgagcatgcc gcctacatgg
     2281 ccaactatta ccgctggttc ggcacccccg aggcgccgtt cggctggtac tacgacctgc
     2341 tggcgttgtg ggcccacgtc agcaccacca gcgtctggat gcggctgccc accctggcga
     2401 tggcgctgac ctgctggtgg gtgatcagcc gggaagtcat gccccgcctg ggtcacgccg
     2461 tcaagaagag tcgctccgcg gcgtggaccg cggcgggcat gttcctggcg gtctggctgc
     2521 cgctggacaa cggcctgcgg cccgagccga tcatcgcgtt gggcatcctg ctgacctggt
     2581 gttcggtgga gcgcgcggtg gccaccagcc ggctgctgcc ggtcgcgatc gcctgcatca
     2641 tcggtgcgtt gaccctgttc tccgggccga cgggcatcgc gtccattggc gcgctgctgg
     2701 tcgcgatcgg gccgttgcgg accatcctgc accgcaggtc caaacggttt ggcgcattac
     2761 cactgctggc cccgatcctg gccgctgcca ccgtcaccgc gatcctgatt ttccgcgacc
     2821 agaccctggc cggcgaggcc caggccagca cgctcaaacg cgccgtcggc ccaagcctga
     2881 gctggttcga cgaacacacc cgctacgagc ggctgttcat ggccagcccc gacgggtcga
     2941 tcgcccgccg cttcgccgtg ctggcgctga ttctcgcgct cggaataacg gtggcggtgt
     3001 cgttgcgtaa gggccgaatt cccgggaccg ccgccgggcc gagccgccgg atcgtcggca
     3061 tcacgatcat ctcgttcctc gcgatgatgt tcacccctac caaatggaca caccacttcg
     3121 gggtgttcgc cgggctggcg ggatcgctgg gggcgctggc cgcggtcgcg gtgacgtccg
     3181 tcgcgatgcg ctcgcgacgc aaccgcaccg tgttcgccgc tgcggtgctg ttcgtggttg
     3241 cgctttcgtt cgccagcgtc aacggctggt ggtacgtatc caatttcggt gtgccatggt
     3301 cgaattcgtt tccaaagtgg cattatgcct tttccaccat cctgctcgcg ctgaccgcac
     3361 tggtgctgct gctggcggcg tggttccact tcgtcttcaa cggcgatgcg ccggcacgcg
     3421 acgggcgcac cggttaccgg gctcgagtga tcgccatcat cgagtccccg ttggcagttg
     3481 caacgtgggt gctggtgatt ttcgaggtgg tgtcactgac cctggcgatg accgagcagt
     3541 acccggcgtg gtcggtggga cgatcgaacc tcgaggctct ggccggcaag tcctgcggac
     3601 ttgccgagga cgtgatggtg gagcaggacc ccaacgccgg catgctgacg ccggtgagcg
     3661 cgtcggtggc tgacgcgctg ggcgcgggcc tcgcggaagc ctttacgccg aacggcattc
     3721 ccgccgacgt ccgcgccgac ccggtgatgg aacgtcccgg cgacctcagc gtcgtcaacg
     3781 aggaattgct ggccaccggc gccgaggccg gcaccgaggg tggcaccaac cccgcgccgg
     3841 gaatcaacgg ttcggtcgcc cagctgccct attacctgga tccggcccgc acgccggtgc
     3901 tgggcagctg gcgctccggg gtgcaggtgc cggccaggtt gcggtcgggc tggtaccggc
     3961 tgcccacacg cgacaaggcg ggcccgctgc tggtggtgag cgcggccggc cgcttcgacc
     4021 cccgcgaggt ccaggtgcag tgggccaccg acgacgaagc cgccagtgga caccctgccg
     4081 gctcgttcca gtttgccgac gtgggggccg cgccggcttg gcgtaatttg cggttgccgc
     4141 tatccgccat cccgaccacc gccacacaga tccgcctggt cgccgacgac gaagatctcg
     4201 cgccgcagca ctggatcgca ctcacgcccc cgcggatccc gcagctgcgc acgctgcagg
     4261 acgtggtggg ctcccgcgac ccggtgctgc tggactggtt ggtcggcttg gcgttcccgt
     4321 gccagcggcc gttcggccat caaaacggcg tcaacgagac accgaaatgg cgcatcctgc
     4381 cggaccgatt cggcgcggag gcaaactcac cggtgatgga taacaacggc ggcggcccgc
     4441 tgggagtgac cgagttggtg gtgaaagcga ccaccgtggc cacctacttg aaggacgact
     4501 ggtcccgcga ctggggctcg ctgcagcgga tgacggccta ctaccccaac gcgcagcccg
     4561 cccgtctgca gttggggacg gcgacacgca gcggtctgtg gagcccggga ccgcttcgcc
     4621 attagatacg caactgcccc gctgcccggt gacgccgttg tcacccggaa acgagacagc
     4681 acgcgctatt tgccccccgc cggcttaggg gacaagggag cgaatcgggt gggtgagggt
     4741 ccagatcggg tgcaggacgt ccgcggcgta gtgccgaatc gggtgcaggg gcccgctgta
     4801 ggggacccac gggtcagccg atgcgacggc cggtacgagc gacccggcga cggacagacc
     4861 gagcgtgccc aacccgacag cggccgccat ccgcagcttc gacaccttct ttgatttggc
     4921 gttcattgcg attcctccct atcgccaagg agtccctgat cagggaaatc cctgtccggg
     4981 gccggccgtg ctgaccgacg accacagtgt tgcgcggggc acttgccagt tctttgcgag
     5041 atgcttgcga aattcttgcg ttcgccagcg ggggtccgtc ggctttatcc gaccgcctgg
     5101 tcacggggct cgggccgcat ctcacgggcc ggtctcggag tgggtcgtgg acgccgcggc
     5161 tcgcggactc cggccccgcc attgcctttg agccacagcc ggatcaccga tccggcatcg
     5221 acccgcgcgc cggactcggg gctctggtcg gtgacgatcc agcctccgtc tccgggggcg
     5281 gtcggaaagt ccgcttcggc attgatcgcc accagcccgt cggcgttgag cacagcacgg
     5341 gcccgcaacc attccaggcc gacgacattg ggcacggtca ccttctgagt tcgccgctct
     5401 tggaggcgca tctgcaccag attcgcggcc acctcggcca cccccgattc agcaccgcgc
     5461 gcctggtcgc cggcatcgtc gaggtcgcgc caccgctccc acgcggattg ccgcgtgaca
     5521 cccaagtgtg tggcgatctc cgcccaggac ttgccatggc gtcgggcggt ccggaccgcc
     5581 gctaactccg cttggtccaa catccgccgc actacgccga tgtcggacag ggcctccaag
     5641 ccgtcgtcgc cgggccgtcc gccacgcgcg gctatccttc gccacgcctg tttggcctgc
     5701 tcccaggcat ccgaagcgct catgatgtca ggatagcctg acacgccggg ggtagccagg
     5761 gccgttgcga agggcattga tggctcacta tcggtaagtc tagacttacc gatagtgagc
     5821 acttaccgag tagcggaaga cggggatgcc tggctggcac tggcggacgg gacgcgacgg
     5881 gccatcgtgg aacgcctagc acgcggcccg tcggccgtcg gtgacctggc tcgcgattta
     5941 ccggtcagca ggccggccgt ctcgcagcac ctcaaagtgc tcaagtccgc cggcctggtc
     6001 cgcgaccgcg cggccggaag gcgtcgcgtc tatcagctcg acccgaccgg cctcgaggtg
     6061 ctgcgcgccg atctcgaccg gttctgggcc caagcgctgg ccacctacgc gcagaccatc
     6121 gacgagacca aaggagagac tccatgacgc agcggcgcag tgtcacgcac catgtcgtcg
     6181 tcaacgcccc gatcgagcgt gcgttcgccg tgttcaccga acggttcggc gacttcaagc
     6241 cccgcgagca caatctgctc gccgtcccga tcgccgagac ggtgttcgag ccgcgggtcg
     6301 gcgggcacat ctacgaccgt ggcgtcgacg gcagcgtctg ccgctgggcg cgcatcctgg
     6361 tgtaccagcc gccccgccgg gtgatcttca cctgggatat cggcccgacc tggcaacggg
     6421 aaaccgatga gtccaagacc agcgaagtcg aagtccgctt taccgccgaa tccgacgagc
     6481 gcacccgtgt cgaccttgag caccgccacc tagagcgcca cggagtgggc tgggagtccg
     6541 tcgccgacgc cgtcgacggc gacgccggct ggccgttgta cctggatcgc tacgacggcc
     6601 tgctcagcac cgaggctccg aaatgaccgc caccctgatg accatggccc acgacgagcg
     6661 ggccgacctg gcggaattcc tggccaccct gacgccgcga gactgggagg cacccagcct
     6721 ctgtaccaag tggagcgtca aagacgttgt cgcgcatgtc atcagctacg aggagctggg
     6781 caccctggga ctggtgaagc gcttcgcgaa gggctggatc gtccggacca accaggtggg
     6841 cgtcgacgag ttcgcggccc tgagcccgca gcagctgctg gagtttctgc gcaatcacct
     6901 ccagccgcac ggactgaccg caggtttcgg cggcatgatc gcgctggtcg acggcacgat
     6961 ccatcaccaa gacatccgcc gtgcgctcgg ccggccgcgc accgtccccg tcgatcggct
     7021 ggagcgggtt ctcggcctgg tgcccggcaa ccccaggctc ggcgccggac gtcggatcag
     7081 gggactgcgg ctgcgcgcca ccgacatcga ctgggcgcac ggccgcggac ccgaggtcac
     7141 cggccccggc gaagcgttgc tgatggcgat gtccggccgc ccggcggcgc tcgccgacct
     7201 acgcggtccg ggcctggaca ccctggcacg acggctgggc gggtagctgg ggatcagccg
     7261 gggcggattt cgaaggtgaa cgcgtggccg ccgatgcgga tgcgggcacc atcggacagg
     7321 gtggcatttg agtggatgcg ctggccctgc acctcgacgc cgttcgtcga tcgcaaatcg
     7381 gtgatcacga agccggtccc ggtgtcgatg atgacggcgt ggtggcggct gacatcctcg
     7441 ccgtcgagga cgatgtcgtt gtcgtcgagg cgcccgagcc gggtggtggc gccgttcaac
     7501 cgatattgac gtccgctctt gtcgcgcagt cgggcgacgg cggcctcggc cagcgccggg
     7561 gcggccgggg cgagcgtgac gctgaggcgc ttgcgcgtgg tcaggtcggc ccgtctaccg
     7621 accaacggct cctgacgcag gatccgctcg tacaaggcgg tgaccgtggg ccccgggtcg
     7681 atgccgagcc cgtcggccag cgccgttttc agtcgcccgt aggcacgcag ggcgtcggac
     7741 tggcgctcgg tgacgtagta ggccgtgatc agctgcgccc acagcggctc gtggtacggg
     7801 tgtgcggcgg tgagcgcctc gagctcaccg atgagcgccc cggggcgtcc gcaggcgatc
     7861 tcggcttccg cgagggcggt gtgggccgcc agtttttctt ccatcagcgt ggtggcgaag
     7921 gggtcgacga agccgaagtc gcgcaggtcg tcgagcaccg gtccgcgcca ttcggcgagc
     7981 gctgccgaca ggtgccggct ggcgtcttcg aaaagtcccg cggcgacagc cttcgtgccc
     8041 gcggcctttg ccgcggtgaa gcggcccacg tcgtagtcgt cgtcagcgac gctcagctga
     8101 taaccgggcg gtgcgctcgc cagcacctgg tacgggtcga cgccggcgtc ccgcagcagc
     8161 cggcgcaggt tggacacgtg ggcctggatg ctggtgcgcg cggcgggcac cgggtcctca
     8221 tcccataccg cgttgatcaa cgcctcgacg gacaccgccc ggttgcggtt gatcaacagc
     8281 accgcgagca ccgctcgctg cttcggagcg cctgcgggca cccggacgcc gtgagaggtc
     8341 agcaacagcg gtccgagggc gccaaacccg agaccagtgg tcgtcatcgc gctgccagcc
     8401 tcccgggtga tcggggccgc ttggagggcc ttggacagcc ctagcacacc attttgaacg
     8461 atgccgggcg ccgagggcct
//
LOCUS       DASSGQ010000323         3266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_904768,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000323 DASSGQ010000000
VERSION     DASSGQ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904768"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1653)
                     /locus_tag="VFI55_14135"
     CDS             complement(<1..1653)
                     /locus_tag="VFI55_14135"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005260951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HET7075206.1"
                     /translation="MNKQFKGTIDIDIRDSVPDWAPFEPPKAPDGAPNVVYIVLDDVG
                     FSAMSCYGGPIETPNIDRIAADGVRYTQWHTTALCSPTRSCLLTGRNHTRNSMACITE
                     AAVGFPNASGTIPPENGMLPEILGEAGWNTYMVGKWHLCPTIEMNVASTRRNWPSGRG
                     FERWYGFLGAETNQWYPDLVYDNHPVDQPRSPQEGYHLTEDLTDKALEFIKDAKTVAP
                     DKPFFLYYAPGAAHAPHHVSKEWADKFKGRFDMGYEAIREQTLARQKQLGIVPADTEL
                     PPINPIGTPETRTGPKGQPFPPLDVTRPWDTLSAEEKRLFCRMAEVYAGFLAHADHHI
                     GRLLDYLEASGQQANTMVILVSDNGASGEGGPDGSVNEMLFANGIPDNMQANLAMLDE
                     LGGTKTYNHYPNGWAMAFNTPFKMWKRYEFNGGTADPCIISWPSATGDATGSGELRHQ
                     YHHAIDIVPTILDTLGVEPPDTIKGHVQSRFDGVSMRYSVEMASAPSARKTQFYSMLG
                     SRGIWHNGWKAVTTHPTLSGWSDFNDDTWELYHTDTDRAEIHD"
     gene            complement(2209..>3266)
                     /locus_tag="VFI55_14140"
     CDS             complement(2209..>3266)
                     /locus_tag="VFI55_14140"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET7075207.1"
                     /translation="WLIDLLGLPSHSSVGFVTGGQMANFTCLAAARHEVLSAVGWDVE
                     ADGLHGAPPVTVVVKQGVHSTVLRALRFLGIGERAVVVVPCDDQDNMRPNALAQALQS
                     IRGPSVITVECGNVNTGAFDDFTAVADLADAHRAAGNPTWVHVDGAVMLLAAASPAMA
                     DRVHGLDRLDSWSTDGHKLLNVAYDCGVAICRNAAAHRAAMSVQASYLEQGTNARDPM
                     DWVPEFSRRARGVGVYATLRSLGRQGVTDLVDRTTRLSHRFADKLSASGRAVIINDVV
                     SNQVLVRWLAPDGDHERLADAVMAGVRADGVAYFSGTTYRGERLMRISVSDWATDEDD
                     VDQAIDALLRHAALATG"
BASE COUNT          497 a         1047 c         1118 g          604 t
ORIGIN      
        1 gtcgtgtatc tcggcgcggt cggtgtcggt gtggtacagc tcccaggtgt cgtcgttgaa
       61 gtcgctccac ccggacaggg tcggatgtgt ggtaaccgcc ttccagccgt tatgccagat
      121 gccgcgagag ccgagcatcg aatagaactg cgtcttgcgc gctgacggcg ccgacgccat
      181 ctcgacgctg tagcgcatgc tcaccccatc gaagcggctc tgtacgtgac ccttgatcgt
      241 gtccggtggc tcgacgccca gcgtgtccag aatggtcggc acgatatcga tcgcgtggtg
      301 atactggtgc cgcagctcgc cgctgccggt cgcgtcacct gtagccgagg gccaggagat
      361 gatgcacggg tcggcggtgc cgccgttgaa ctcgtaccgc ttccacatct tgaacggcgt
      421 gttgaatgcc atcgcccaac cgttggggta gtggttgtag gtcttggtcc cgccgagctc
      481 gtccagcatt gccaggttgg cctgcatgtt gtccgggata ccgttcgcga acaacatctc
      541 gttgacggag ccatccgggc cgccttcgcc gctggccccg ttatcggaca ccaggatcac
      601 catggtgttc gcctgctgcc cggacgcctc caggtagtcc agcagccggc cgatgtggtg
      661 gtcggcgtgc gccaggaacc cggcgtacac ctcggccatc cggcagaaca gccgcttctc
      721 ctccgccgac agtgtgtccc acggcctggt gacatccagt ggcgggaacg gctgaccctt
      781 gggccccgtg cgggtctcgg gtgtgccgat cgggttgatc ggaggcaact cggtgtcggc
      841 cgggacgata cccagctgct tttgccgggc cagggtctgt tcccgaatcg cctcataccc
      901 catgtcgaag cggcccttga acttgtcggc ccattccttg gagacgtggt gcggcgcgtg
      961 ggccgcaccc ggcgcgtagt acaggaagaa cggcttgtcg ggcgcgaccg tcttcgcatc
     1021 cttgatgaat tccagcgcct tgtcggtgag atcctctgtc aggtgatagc cctcctgcgg
     1081 tgacctcggc tggtcgacgg ggtggttgtc gtacaccagg tccgggtacc actggttggt
     1141 ctcggcgccc aggaacccgt accagcgctc aaacccgcgg ccggagggcc agttccgccg
     1201 cgtcgaggca acattcatct cgatggtcgg gcacaggtgc cacttgccga ccatgtacgt
     1261 gttccagccg gcctcgccga ggatctccgg gagcatgccg ttctccggcg ggatcgtccc
     1321 gctcgcattc gggaacccga ccgcggcctc ggtgatgcac gccatcgagt tgcgggtgtg
     1381 gttgcgcccg gtcagcaggc acgagcgggt cggcgagcat agcgccgtgg tatgccactg
     1441 cgtgtaccgc accccgtcgg cagcgatgcg atcgatgttg ggcgtctcga tcggcccgcc
     1501 gtaacagctc atcgccgaga agcccacatc gtcaagcacg atgtaaacca cattgggtgc
     1561 gccgtccggc gccttcggcg gctcgaacgg agcccagtcg ggaaccgagt ccctaatatc
     1621 gatatcgatc gtgcccttga attgcttgtt cacgtcaccg ctccttcccc gagacgacgg
     1681 gtgactggtt ctgcgttgga ttctggttcc gagcactgac cgcggcacga gcttgcagcg
     1741 gcagacatcc gcccgctcta tcgtggccga ccgacatcgt taccggctca ggggtgccta
     1801 gtgagactgc actgggggag cggcttggat gtaggccagt tgctcgggcc gactggcccg
     1861 cacctgtgcg agtgcaggct gagcgttggc cagcgcacgg cctggttctg gtccccagtc
     1921 gccgggccgc gccgaagatc atcgagaatg gtcatcgtgc gggccgatcc gggccagtct
     1981 cggtcgtcaa tcgccacgag cgaagctggc attggctgcc agcctgcgat ataccctcag
     2041 cgagactgac cctactccac gcactgcatc cgctcgtcgg cgaatccagc tgctcagccg
     2101 gctcctccag ttggtccgcg ggtcgggtgg gagaggattc cgcgttcagg gattggaggc
     2161 gcgtgcgagg ctctggtcgg gcctggcacc gcatggcggt taggttgctc atccggttgc
     2221 gagagcggcg tgtcgcagca gtgcgtcgat ggcctggtcg acgtcgtctt cgtcggtggc
     2281 ccagtccgag accgagatac gcatgaggcg ttcgccgcgg taggtggtgc cgctgaagta
     2341 ggcgacgccg tcggcgcgga cgccggccat gacggcatcg gccaggcgct cgtggtcgcc
     2401 gtcgggcgca agccatcgca cgagcacctg gttggacacg acatcgttga tgatcaccgc
     2461 gcgaccgctg gcgctgagct tgtcggcgaa ccggtgcgac agtcgagtag ttcggtcgac
     2521 aaggtcggtg acaccctgcc ggccgaggga tcgtagggtt gcgtacacgc ccacgccgcg
     2581 ggcgcggcgg gagaattcgg gcacccagtc catcggatcg cgtgcgttcg tgccctgttc
     2641 gaggtaactg gcctgcacgc tcatcgcggc ccggtgcgcg gctgcgttcc ggcagatcgc
     2701 gacgccgcaa tcgtaggcga cgttgagcag cttgtgtccg tcggtggacc aggagtcgag
     2761 tcggtcgagg ccgtggacac gatcggccat ggcgggtgat gcggcggcca gtagcatcac
     2821 cgcgccgtcg acgtgcaccc aggtgggatt gccggccgcg cggtgcgcgt cggcgaggtc
     2881 ggcgaccgcg gtgaagtcgt cgaacgcgcc ggtgttcacg ttgccgcact cgacggtgat
     2941 gacagagggc cccctgattg actgcagggc ctgcgcgagc gcgttcgggc gcatgttgtc
     3001 ctggtcgtcg cacggcacaa cgacgacggc gcgttcgccg atccccagaa aacgcaacgc
     3061 gcgcagcacg gtcgagtgca caccctgctt gaccacgacg gtgacgggcg gggcgccgtg
     3121 tagaccgtcg gcctcgacgt cccacccgac cgcgctcagc acctcgtgac gtgcggcggc
     3181 cagacaggtg aagttggcca tctgtccgcc ggtgacgaag ccgaccgagc tgtgcgacgg
     3241 cagcccgagc aggtcgatga gccatt
//
LOCUS       DASSGQ010000324         6106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_388513,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000324 DASSGQ010000000
VERSION     DASSGQ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388513"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VFI55_14145"
     CDS             <1..463
                     /locus_tag="VFI55_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET7075208.1"
                     /translation="DSRVSAELVFDQGLGDLFVVRTAGQAIDSAVLGSIEYAVTVLNV
                     PLIVVIGHDSCGAVKAAVSALDEGAIPGGFVRDVVERVAPSILMARRDGLSRIDEFEE
                     RHVRETVAQLMGRSSAIAERVAAGTLGIAGITYRLAEGRAELRDKVGDIGE"
     gene            579..1385
                     /locus_tag="VFI55_14150"
     CDS             579..1385
                     /locus_tag="VFI55_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075209.1"
                     /translation="MLDLEPRGPLPTEIYWRRRGLALGVAVVVIGIAAAIVIAFMSNS
                     AGAKPANADKSNSAQSKPGSPPPEAPPPPGQEGVVPLAPPQGQNPESPTATAAVQPPP
                     VLKEGDDCPDSTLAVKGLTNAPQYFIGDQPKFTMVVTNIGLVPCKRDVGAAVLAAYVY
                     SLDNKRLWSNLDCAPSNETLIKTFTPGEQVTTAVTWTGMGSAPHCPLPRPAIGPGTYN
                     LVVQLGNLRSQPLPFILNQPPPPPGPVPGQPAMAPPPEAPPVAPAPLPGG"
     gene            complement(1408..2487)
                     /gene="disA"
                     /locus_tag="VFI55_14155"
     CDS             complement(1408..2487)
                     /gene="disA"
                     /locus_tag="VFI55_14155"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HET7075210.1"
                     /translation="MHAVTRPTLRETVARLAPGTGLRDGLERILRGRTGALIVLGNDE
                     NVESICDGGFALDVRYAPTRLRELAKMDGAVVLSTDGSRIVRANVQLVPDPSIPTDES
                     GTRHRSAERAAIQTGYPVISVSHSMNIVTVYVAGERHVVADSATILSRANQAIATLER
                     YKTRLDEVSRQLSRAEIEDFVTLRDVMTVVQRLELVRRIGLVIDYDCVELGTDGRQLR
                     LQLEELLGGNEMALELVVRDYHASPEPLSRAQLGATLDELDALSDAELLDFTSLAKVF
                     GYPTTTDAQDSALSPRGYRAMAGIPRLQFAHADLLVRAFGTLQGLLAASASDLESVDG
                     IGAMWARHVREGLSQLAESTIADPL"
     gene            complement(2492..3928)
                     /gene="radA"
                     /locus_tag="VFI55_14160"
     CDS             complement(2492..3928)
                     /gene="radA"
                     /locus_tag="VFI55_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET7075211.1"
                     /translation="MAQARSQYRCSECQHATAKWVGRCLECGTWGTVDEVPVLGVVGA
                     GRRRVDPASRPVPISSIEAHQTRHRPTGIGELDRVLGGGVVPGSVTLLAGDPGVGKST
                     LLLKVAHRWAQAGRRALYISGEESAGQIRLRADRMECSADEVYLAAESDLHTVLDHIA
                     TVRPALVIVDSVQTMSTTEADGVAGGVTQVRAVTAGLTAAAKAQGVALILVGHVTKDG
                     AIAGPRSLEHLVDVVLHFEGDRNGTLRMVRGIKNRFGAADEVGCFMLHDNGIEDVTDP
                     SHLFLDQRPAPVAGTAITVTLDGKRPLIGEVQALLATPSGGSPRRAVSGIDHSRAAMI
                     TAVLEKHARLNIAVNDIYLSTVGGMRLTDPSSDLAVAIALASAYANLPLPTTAVMIGE
                     VGLAGDLRRVSGMARRLGEAARQGFNIALIPPGCDARRDIPPGMRALTAPTVVAALEH
                     MIDIADHRSGTPATPQPLDTSPCAPTRA"
     gene            complement(3978..4523)
                     /locus_tag="VFI55_14165"
     CDS             complement(3978..4523)
                     /locus_tag="VFI55_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075212.1"
                     /translation="MRRSAPVVSVARVVAVALVTLVAALLSGCGAGQISQMATQEPAV
                     NGNKVTINNVALRDIRMQAVQTSDFLQPGRTVDLVAVAINQSPDIADRLVGITTDIGT
                     VTVSGEGRLPASGMLFIGKPDGQNVAPGPPDSNNAVRATVALTKPITNGLTYNFTFNF
                     EKAGQASVLVPISAGVGPQPI"
     gene            4842..5330
                     /gene="carD"
                     /locus_tag="VFI55_14170"
     CDS             4842..5330
                     /gene="carD"
                     /locus_tag="VFI55_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     CarD"
                     /protein_id="HET7075213.1"
                     /translation="MIFKVGDTVVYPHHGAALVEAIETRTIKGEQKEYLVLKVAQGDL
                     TVRVPADNAEYVGVRDVVGQEGLDKVFQVLRAPHTEEPTNWSRRYKANLEKLASGDVN
                     KVAEVVRDLWRRDQERGLSAGEKRMLAKARQILVGELALAESTDDAKAETILDEVLAA
                     AS"
     gene            5347..6033
                     /gene="ispD"
                     /locus_tag="VFI55_14175"
     CDS             5347..6033
                     /gene="ispD"
                     /locus_tag="VFI55_14175"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET7075214.1"
                     /translation="MAGATGSVVAVVPAAGSGERLAAGVPKAFYQLEGRTLLERAVDG
                     LLDSGVVDTIVVAVPPGLIDEGKLILGDKATVVAGGTNRTESVSLALSALPEAATPAF
                     VLVHDAARALTPPHLIVRVVDALRAGHGAVVPALPLSDTIKAVDANGAVLGTPERAGL
                     RAVQTPQGFDTDLLLRAYRAYRAAAGFTDDASLVEHVGGQVQVVDGDPLAFKITTQLD
                     LLLAEAIVRR"
BASE COUNT          977 a         2047 c         2110 g          972 t
ORIGIN      
        1 ggacagccgg gtatcggccg aactcgtctt cgaccagggc ctcggcgacc tgttcgtggt
       61 tcgcacagcg ggtcaggcca tcgattccgc ggttctgggc tccatcgagt acgccgtgac
      121 ggtgctcaac gtgccgctca tcgtcgtgat cggccatgac agctgcggtg cggtcaaggc
      181 cgccgtgagt gcgctcgacg aaggcgcaat accaggaggt ttcgtgcgcg atgtggtcga
      241 acgcgtcgct ccatcgatcc tgatggcgcg acgcgacggg ctgagccgca tcgacgagtt
      301 cgaggagcgg cacgtacggg agacggtcgc gcagctcatg gggcgttctt cggccatcgc
      361 ggagcgggtg gccgcgggca ccctgggcat cgcgggcatc acctatcgcc tcgccgaggg
      421 gcgggcggag ttgcgcgaca aggtcggtga catcggcgag tgacgtccct tcgccccggc
      481 cgcccgaccg tctcctaaca cgctggctgg ccagcaaacc gggcgacacg ccgaggcggg
      541 tcgaacaaat ccgcgtgacc tggccctacg gtgtgaacgt gctggatctg gaaccgcgag
      601 gcccgctacc taccgagatc tattggcggc gcagaggctt ggccctcggt gtcgcggtcg
      661 tcgtgatcgg gatcgcggcc gccatcgtca tcgccttcat gagcaacagt gcgggggcga
      721 aacccgccaa cgccgacaaa tccaactccg cgcaaagcaa gccgggttcg ccgccacccg
      781 aggcgccgcc cccaccgggg caggagggcg tcgttccgtt ggcgccgccg caggggcaaa
      841 accccgagtc gcccacggcg accgccgcgg tgcagccgcc gccggtgctg aaggagggcg
      901 acgactgccc cgattcgacg ctggccgtca agggcctgac gaacgcgccg cagtacttca
      961 tcggcgacca gccgaaattc accatggtcg tgacgaacat cgggctggtg ccctgcaagc
     1021 gcgacgtcgg agccgcggtg ctggccgcct acgtctattc gctggacaac aagcggctgt
     1081 ggtccaacct ggactgcgcg ccgtccaacg agaccctgat caaaaccttc acgccgggcg
     1141 agcaggtgac gacggcggtg acctggaccg gcatggggtc ggcgccgcat tgcccgctgc
     1201 cgcggccggc gatcgggccg ggtacctaca acctcgtcgt gcagctgggc aacctgcgct
     1261 cgcagccgct gccgttcatc ctgaatcagc cgccaccgcc gccggggccg gtgccgggcc
     1321 agccggcgat ggccccgccg ccggaggcac cgccggtcgc tccggctccc cttccggggg
     1381 ggtaacccgg gctcagtgcc cgtctggcta tagcgggtcg gcgatggtcg actcggccag
     1441 ttgcgacagc ccctcccgca cgtggcgggc ccacattgcg ccgatgccat ccaccgattc
     1501 caggtcgctg gcgctggccg ccagcaggcc ctgcagcgtt ccgaatgccc ggaccaacag
     1561 gtccgcgtgc gcgaactgca gccgggggat gccggccatc gcgcggtagc cgcgcgggct
     1621 cagcgccgag tcctgcgcgt ccgttgtcgt cggatagccg aaaacctttg ccagcgaagt
     1681 gaagtcgagc agttcggcgt ccgacagggc atccagctcg tcgagggtgg cgcccagttg
     1741 cgctcgagac aatggctcgg ggctggcgtg atagtcgcgc acgaccagct ccagggccat
     1801 ttcgttgccg cccagcagct cctcgagctg gagtcgtaac tggcgcccgt cggtgccgag
     1861 ttcgacgcag tcgtagtcga tgaccagccc gatccgccgg accaactcga gccgctgcac
     1921 caccgtcatc acgtcgcgaa gcgtgacgaa gtcttcgatc tccgcgcggg acagctgccg
     1981 gctgacctca tcgagcctgg tcttgtaccg ctccagggtg gcgatggcct gattggcccg
     2041 cgacaggatg gtcgcggaat cggcaaccac atggcgctcg ccggcgacgt agacggtgac
     2101 gatgttcatc gagtggctga ccgagatcac cgggtagccg gtctggatcg cggcccgctc
     2161 cgcggagcgg tggcgggttc ccgattcgtc ggtggggatc gacggatccg gaaccaactg
     2221 cacgttggcc cgcacgatgc ggctgccgtc ggtggacagc acgacggcgc cgtccatttt
     2281 cgccaactcg cgcagccgtg tcggcgcgta ccggacgtcg agcgcaaaac cgccgtcgca
     2341 aatggactcg acgttctcgt cgttgccgag cacgatcaac gcaccggtac ggccgcgcag
     2401 gatgcgctcg aggccgtccc gtagcccggt gcccggcgcc aggcgggcga cggtctcacg
     2461 cagggtcgga cgggtcacag cgtgcattct gctacgcgcg cgttggcgcg caaggggaag
     2521 tgtccagcgg ttgtggcgtt gcgggcgtgc cactgcggtg gtcggcgatg tcgatcatgt
     2581 gctccagcgc ggcgacgacg gtgggcgcgg tcagcgcgcg catgcccggc gggatgtcgc
     2641 gtcgggcgtc gcagccgggt gggatgagcg cgatgttgaa accctgacgt gcggcttcgc
     2701 ccagccgccg ggccataccg ctgacccgcc gcaggtcgcc cgccagcccc acctcgccga
     2761 tcatcaccgc cgtggtgggc agcggcaggt tcgcgtatgc cgacgccagc gcgatggcga
     2821 cggcaagatc cgaggacggg tcggtcaacc gcatgccgcc cacggtggac aggtagatgt
     2881 cgttcacggc gatgttcaac ctggcgtgct tctccagcac cgcggtgatc attgcggccc
     2941 gggagtggtc gatgccgctc acggcgcgac gcggcgagcc acccgacggg gtcgccagca
     3001 acgcctggac ttcgccgatc agcggccgct tgccgtccag cgtcaccgtg atcgcggtac
     3061 cggcgaccgg tgccggccgc tggtccagga ataggtgcga cgggtcggtc acgtcctcga
     3121 tcccgttgtc gtgcagcatg aaacacccga cttcgtcggc cgcaccgaac cgattcttga
     3181 tcccgcggac catccgcagc gtgccgttgc ggtcgccctc gaaatgcagc acgacgtcga
     3241 cgagatgttc cagggagcgt ggtcccgcga tggccccgtc cttggtgacg tgaccgacaa
     3301 ggatcagggc gacaccttgg gccttggcgg ccgcggtcag cccggcggtc acagcgcgca
     3361 cctgcgtgac gccgccggcc acgccgtcgg cctcggtggt ggacatggtc tgcaccgagt
     3421 ccacgatgac cagcgcgggg cggaccgtcg caatgtggtc cagcacggtg tgcaggtcgg
     3481 attcggcggc cagataaacc tcgtcggcgc tgcattccat ccggtcggcg cgcagccgga
     3541 tctggccggc ggactcctcg ccggagatat acagcgcccg acggcccgcc tgcgcccagc
     3601 ggtgggcgac cttgagcaac aacgtcgact taccgacccc cggatcgccc gccagcagcg
     3661 tgaccgagcc gggcaccacg ccacccccga gcacgcggtc gagttcgcct atgcccgtgg
     3721 ggcggtgtct cgtctggtgc gcttcgatgg aactgatcgg aacgggccgc gaagccggat
     3781 ccacgcggcg gcggccagca ccgacgacgc cgagcaccgg cacctcgtcg acggtgcccc
     3841 aggtgccgca ctccaggcag cggcccaccc atttggcggt ggcgtgttgg cattccgagc
     3901 aacggtattg cgagcgtgct tgtgccacgc catgaccgta tccgtccggt gcgacaagct
     3961 gggcgtgaca ggccgcctta gatcggctgg ggacccactc cggccgaaat cgggaccagc
     4021 acgctggcct gcccggcctt ctcgaaattg aaggtgaagt tgtaggtcag gccgttggtg
     4081 atgggcttgg tcaaggcgac ggtcgcccgg accgcattgt tggagtcggg gggtccgggc
     4141 gccacgttct ggccgtcggg tttgccgatg aacagcatcc cggaggcggg cagtcgacct
     4201 tcgccgctga ccgtcaccgt gccgatgtca gtggtgatgc ccaccagcct gtcggcgatg
     4261 tcgggcgact ggttgatggc caccgccacc agatccaccg tgcggccggg ttgcaggaag
     4321 tcgctggtct gcacggcttg catgcggatg tcacgcaacg ccacgttgtt gatcgtgacc
     4381 ttgttgccgt tgacggcggg ctcctgggtg gccatctgcg agatctgacc ggctccgcaa
     4441 ccgctgagca gggcggcgac cagggtgacc agagcgacag cgacgacccg ggcgacactg
     4501 acgaccggcg ccgaacgtcg gatcgggagg ctgatcttga agcggctcac tcactgcctc
     4561 ctgctcggct tggcaccacc acaacggcga ttcagcgatt caactatgca cagtagtagg
     4621 aacggcctgc cagcggtagc gcagggcacc agagcagccc ggccggcccg ggtggggtgc
     4681 ccgccggggg taaaaagcca cgttggtagt gtgtttgctt gcacgtcttg ctccgcctgt
     4741 caacccctaa tctatcccgg ttgtgcccct gacctgcaac gttgttccgc aagcctgggc
     4801 gcgacgtgtt aggatgaagt aacgaaaggg gctcgaatca gatgatcttc aaggtcggcg
     4861 acaccgttgt ctatccacac cacggtgctg cgttagtcga ggcgatcgaa acccggacca
     4921 tcaaagggga acaaaaagag tatctcgtct tgaaagtggc gcagggcgac ctgacagttc
     4981 gagtacccgc cgacaacgcc gagtacgtcg gtgtccgcga cgtcgtgggc caggagggcc
     5041 tcgacaaggt cttccaggtt ttgcgcgctc cccacaccga agagccgacc aactggtccc
     5101 gccgctacaa ggccaacctc gaaaaactcg cctccggcga cgtcaacaag gtcgccgagg
     5161 tagtgcgcga tctgtggcgc cgcgaccagg agcgcggcct gtccgccggc gagaagcgga
     5221 tgctggccaa ggcccggcag attctggtcg gtgagctggc gctcgccgaa agcaccgacg
     5281 acgccaaagc ggagaccata ctcgacgagg tgctggccgc cgcttcttga aggccctgag
     5341 ggtctggtgg ccggggcaac gggcagcgta gtcgcagtgg tcccggccgc ggggtcgggg
     5401 gagcggctcg ccgccggcgt acccaaggcg ttctatcagt tggaggggcg caccctcctc
     5461 gaacgggccg tagacggcct gctggattcc ggggttgtcg acaccatcgt ggtggccgtc
     5521 ccgccgggcc tcatcgacga gggaaagctg atcctcggtg acaaggccac cgtcgtggca
     5581 ggcgggacaa accgtaccga atccgtgagc ctggccctgt ctgcgttgcc ggaagccgcg
     5641 acaccggcgt tcgtgctggt gcacgacgcg gcccgggccc taacaccgcc acatctgatc
     5701 gtgcgcgtgg tcgacgcgtt gcgtgccggc catggcgccg tcgtgcccgc gctgccgctg
     5761 tctgacacca tcaaggccgt ggatgccaac ggcgcagtct tgggcacccc ggagagggcc
     5821 gggctgcggg cggtgcagac tccgcaaggc ttcgacaccg atctgctgct gcgcgcctac
     5881 cgggcttatc gcgccgccgc cggcttcacc gacgacgcat cgctggtcga gcacgttggc
     5941 ggtcaggtcc aggtggtcga cggcgacccg ctggccttca agatcaccac tcaacttgat
     6001 ttgttgctgg ccgaagcgat cgtgcgccgg tgaccgagct gcttcccctt ccgaggatcg
     6061 gattgggcac cgacgtgcat ccgatcgaat ccgggaggcc gtgctg
//
LOCUS       DASSGQ010000325         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_844698,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000325 DASSGQ010000000
VERSION     DASSGQ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844698"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VFI55_14180"
     CDS             complement(<1..530)
                     /locus_tag="VFI55_14180"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET7075215.1"
                     /translation="MLGAIGAGYSRYVAIGDSQTEGLWDGDDVVGLLGFADRLAAMID
                     SVYPGLQYANLAIRGKRMADVLGEQVPPALAMRPDLITVCAGMNDVIQPGRSFGRALG
                     DLEDTYSALAQSGATVVTTTFPNVAQFLPLGRIVSRRLSRINNAISAAAHRYGFRLVD
                     LYNAPSMRNVETWAVDR"
     gene            615..806
                     /locus_tag="VFI55_14185"
     CDS             615..806
                     /locus_tag="VFI55_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075216.1"
                     /translation="MATPRKVTSLRTWGRAEVAIGVSVTLVVFVAGLLWAKWMPYAAK
                     AAKADRTHHWSGSNVLTVL"
     gene            803..1087
                     /locus_tag="VFI55_14190"
     CDS             803..1087
                     /locus_tag="VFI55_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075217.1"
                     /translation="MSQPAHRGLLVVVLAAIAGTLIVIPAAGEIPILQGLALLGVSSG
                     TLGALLITLPAVSAPGAAMVARSFGWKAIATATFVVIMAGLLGAAVLSLL"
     gene            complement(1082..1762)
                     /locus_tag="VFI55_14195"
     CDS             complement(1082..1762)
                     /locus_tag="VFI55_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7075218.1"
                     /translation="MSDRSTQAAYLHASRGRRSSHHSGDNREQAILATAERLLQERPL
                     ADFSVDDLARGAGISRPTFYFYFQSKNAVLLSLLDQMNSKAHAALTALGATLSGDPAT
                     LWRSRIEAFFEVSGSHRAVAVAGAAAKATNPEVRQLWSTLMQKWIAYTTAAIKAERQR
                     GAAPDTFLAEDLSVALNLLNERVMAATFTDEEPAIPEDRVIDTLVHIWLASIYESTGP
                     VAPVPADS"
     gene            2015..>2623
                     /locus_tag="VFI55_14200"
     CDS             2015..>2623
                     /locus_tag="VFI55_14200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7075219.1"
                     /translation="MPCIDRRTVLCAIACGVLASITRAQIAGASALRWNIPLGPVPAP
                     SGVLSRVPGDSNRIVLTVDDGASTPVVAAFAQFAQDSGTRLTFFVNGANPSWSVNSPA
                     LRPMVDSGQVQMANHTWSHPNLSHLGLSAVGDQIRRNADFLSNTYGGDGTPFFRPPFG
                     AHNADIDRVAADQGYTSITMWSGDVGDSAPENESSLVTRASKS"
BASE COUNT          427 a          831 c          892 g          473 t
ORIGIN      
        1 cggtcgacgg cccacgtctc cacgttgcgc atcgacggcg cgttgtacag gtcgacgagc
       61 ctgaacccgt agcgatgggc ggccgcggag atcgcgttgt tgatgcggct gagccgcctc
      121 gacacgatcc ggccgagcgg aaggaactgc gcgacgttcg ggaaggtcgt cgtcaccacc
      181 gtcgcccccg attgggccag cgcggagtag gtgtcttcga ggtcgccaag cgcacggccg
      241 aacgatcgtc ccggctggat gacgtcgttc attccggcgc agacggtgat caggtccggc
      301 cgcatcgcca gcgccggcgg aacctgctcg ccgagcacgt cggccatccg cttgccgcgg
      361 atcgccaggt tggcgtattg caaacccggg tagaccgaat cgatcatcgc ggcgagccgg
      421 tcggcgaatc cgagcaggcc aacgacgtcg tcgccatccc acaagccctc ggtctggctg
      481 tcgccgatgg cgacgtatcg gctgtatccg gctccgatcg ccccaagcac gaagccgaga
      541 ctaatcgcac ctttgtggtg agctgcaatg tggcgtggct cgatacgagt gcgcctgggt
      601 tagcgtcctc gtctatggcc acgccgcgga aggtcacttc gcttcggacc tggggtcgcg
      661 cggaggtagc catcggcgtt tcggtgacgc tggtggtctt cgtcgccggg ctgctctggg
      721 ccaagtggat gccgtatgcg gcgaaagccg ccaaggccga tcggacccat cactggtcgg
      781 gttccaacgt cctcacggtt ctttgagcca gccggcgcac cgcggcctgc tggtcgtcgt
      841 actcgccgcg atcgcgggca cgctcatcgt gatccccgca gcaggggaga ttcccatcct
      901 gcagggcctg gcgctgctcg gggtctcgtc gggaacgctc ggtgccctgc tgatcaccct
      961 tccggccgtc agcgctccgg gcgccgcgat ggtcgcccgc agcttcgggt ggaaggccat
     1021 cgcgacggcg acgttcgtgg tgatcatggc cgggttactc ggcgcggcag tgctgagcct
     1081 gttatgagtc cgcgggcacc ggggcaaccg ggccggtgga ttcgtagatg ctggccagcc
     1141 agatgtgtac cagcgtgtcg atcacccggt cttcggggat ggcgggctcc tcgtcggtga
     1201 acgtcgcggc catcacccgt tcgttcagca ggttcagtgc gacggacagg tcctcggcga
     1261 ggaaggtgtc gggcgcggcg ccgcgttggc gctcggcctt gatcgccgca gtggtatagg
     1321 cgatccactt ctgcatgagg gttgaccaca gctgacggac ttcggggttg gtggctttgg
     1381 ccgccgcgcc ggccacggcg accgcccggt gcgacccgga cacctcgaag aaggcctcga
     1441 tgcgcgaccg ccacagcgtc gcgggatcgc ccgacagcgt cgcgcccagg gccgtcaacg
     1501 ccgcgtgtgc cttgctgttc atctggtcga gcagcgacaa caacaccgcg tttttcgact
     1561 ggaagtaaaa gtagaaggta ggccgcgaga tgcccgcgcc tcgggccagg tcatcgaccg
     1621 agaaatcggc cagcggccgc tcctggagga ggcgttcggc ggtggccagg atcgcttgtt
     1681 cgcggttgtc gcccgagtgg tgcgaggaac gccggccacg cgaggcgtgc aggtacgcgg
     1741 cctgcgtcga gcggtcggac acgtgagtta ctttatggac acgtcgatat acatcaacga
     1801 aattgttgat taaaaccaac gatattgtcg attaagattg gcggtttgtt tctcggccaa
     1861 tttgtttaat ggtcgacggc tcgtcgaaat tcgtatcatc acaacctggt cgtgggggag
     1921 accttcgggc cgtgctgaca cgcggtgagg cgggtgggcg gacaaagcga cccgaaaaag
     1981 attcagcgtg cgccatgcgg ggaacaacga ttgcatgccg tgtatcgatc ggcgcacggt
     2041 cctctgcgcg attgcctgcg gagtattggc gagcatcacc cgtgcacaaa tcgccggcgc
     2101 gtcggccctt cgttggaaca tcccgttggg gccggtcccg gcgccctcgg gcgtcctcag
     2161 ccgggtgccc ggcgacagca atcggatcgt gctgaccgtt gacgacggcg ccagcacgcc
     2221 ggtggtggcg gcgttcgcgc aattcgcgca ggacagtgga acccggctga cctttttcgt
     2281 caacggcgcc aacccttcat ggtcggtcaa ctctccggcg cttcgcccga tggtggattc
     2341 cgggcaggtt caaatggcga accacacctg gtcgcacccg aacctcagtc acctggggct
     2401 gagcgcggtg ggcgatcaga tccgacgcaa cgccgatttc ctgagcaaca cctacggtgg
     2461 cgacggcact ccgttcttcc ggccgccctt cggggcccac aacgccgaca tcgaccgggt
     2521 ggccgccgat caggggtaca cctcgatcac catgtggagc ggggacgtcg gtgactcggc
     2581 acccgagaac gaaagcagcc tcgtgacccg tgcgtccaag tcg
//
LOCUS       DASSGQ010000326         2842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1299681,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000326 DASSGQ010000000
VERSION     DASSGQ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299681"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFI55_14205"
     CDS             <1..534
                     /locus_tag="VFI55_14205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7075220.1"
                     /translation="LDSLIGNLLDVTRIHTGAVQPFIRPTALDEVAPLVVGGLDGGER
                     VRFDIPDALPLLATDPGLLERALANLIGNALRYSPADQPPTLTAAVRDGTVTVRVVDH
                     GPGIPPDQRDHVFEPFQQLGDQHTSGGVGLGLAVAKGFLDAVGGQLTAEATPGGGLTM
                     RIDLPVATVHDRTGVVR"
     gene            531..1211
                     /locus_tag="VFI55_14210"
     CDS             531..1211
                     /locus_tag="VFI55_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7075221.1"
                     /translation="MTSVLVIDDETALLRALRINLTARDYTVTTAADGATGLAAMAHH
                     RPDVVIVDLGLPDMDGTDVIRGIRGWSTTPIIVLSARGEEIQKVDALDAGADDYITKP
                     FGMNELLARVRAATRRGTPAVEEPLVATGQFSIDLAAKRVTTTAGEDIRLTPTEWQLL
                     EVLARHRGRLVTGRQLLHDVWGPHYATETHYLRVYIAQLRRKLEPDPAHPHYLITEPG
                     VGYRLQAE"
     gene            1301..1468
                     /locus_tag="VFI55_14215"
     CDS             1301..1468
                     /locus_tag="VFI55_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075222.1"
                     /translation="MAERQLELNDDGGLRGLQRSASAQRAGLFTGAAVALSDAPSIGI
                     CWGPLIDQRTS"
     gene            complement(1453..2358)
                     /locus_tag="VFI55_14220"
     CDS             complement(1453..2358)
                     /locus_tag="VFI55_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020105224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7075223.1"
                     /translation="MDNRAQIREFLASRRARITPDQAGLPAYGGNRRVKGLRREEVAL
                     LAGVSIDYYIRMERGDLAGASEAVLDGVANALQLDEAEREHLIALARSAGATSTRRAR
                     PSPTTVRPVIQQVLDAITEAPAWIRNARHDILAMNPLARALYSPVLDSAVAGATTKRA
                     ANTTRFIYLDPAAQEFFIDYDRIATDAAAMLRLEAGRNPHDKDLITLVGELSTQSELF
                     RQHWASHNVKFHRTGRKRLRHPAVGELDLNFEAMELPSEPELRLNIYTADPHTPTADG
                     LKLLASWAATRAVDSPDTQHAHDVR"
     gene            2460..>2842
                     /locus_tag="VFI55_14225"
     CDS             2460..>2842
                     /locus_tag="VFI55_14225"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HET7075224.1"
                     /translation="MSTVTETADNRHIDRESLKVLARAVTASNGWGADELATIDRTGE
                     VDVATRRGDGTLRAARIVWIVRHGDAAYVRSVNGTSAAWYRGVQTRHEGELGAGRLRR
                     DVAFVEAGEHAGDSSGLDDALDAAYR"
BASE COUNT          435 a          974 c          981 g          452 t
ORIGIN      
        1 ctcgactcgc tgatcggcaa cctgctcgac gtgacccgca tccacaccgg cgcggtgcaa
       61 ccgttcatcc gtcccaccgc cctcgacgag gtcgccccac tggtcgtcgg cggactcgac
      121 ggcggtgaac gggtgcgctt cgacatcccc gacgccctgc ccttgttggc caccgacccc
      181 ggcctgctcg aacgcgctct ggccaacctg atcggcaacg cgctgcgtta ctcgcccgcc
      241 gaccagccgc ccaccctgac cgcagcggtc cgcgacggga ccgtcactgt ccgggtcgtc
      301 gaccacgggc cgggcatccc acccgatcag cgagaccacg tcttcgaacc gttccagcaa
      361 ctcggcgatc agcacaccag cggcggtgtc ggcctcggcc tggccgtcgc caaaggcttc
      421 ctcgacgccg tcggcgggca gctcaccgcc gaagcgacac ccggcggcgg actcaccatg
      481 cgcatcgacc tgcccgtcgc caccgtccac gaccgcaccg gcgtcgtccg gtgaccagcg
      541 tgctggtcat cgacgacgaa accgcactgc tgcgcgcgct gcggatcaac ctcaccgccc
      601 gcgactacac cgtcaccacc gcagctgacg gtgccaccgg gctcgccgcc atggcgcatc
      661 accgacccga cgttgtcatc gtcgacctcg gcctgcccga catggacggc accgacgtca
      721 tccgcggtat ccgcggctgg tccaccaccc cgatcatcgt gctgtccgcc cgcggtgagg
      781 aaatccagaa agtcgacgcc ctcgacgccg gcgccgacga ctacatcacc aaacccttcg
      841 gcatgaacga actgcttgcc cgagtccgcg ccgccacccg ccgcggcaca cccgcagtcg
      901 aagaacccct cgtcgctacc ggccagttca gcatcgatct cgccgccaaa cgggtcacca
      961 cgacagccgg cgaggatatc cggctcaccc caacggaatg gcagctgctt gaagtccttg
     1021 cccgccaccg cggccgactc gtcaccggcc ggcaactcct ccacgacgtc tggggtcccc
     1081 actacgccac cgaaacccac tatctgcgcg tctacatcgc ccaactacgc cgcaaactcg
     1141 aacccgaccc agcccacccc cactacctga tcaccgaacc tggcgtcggc tatcgcctgc
     1201 aggccgaatg atcctcgggt ggggggcgtg cactgaatag cttggccggc gctcatgatg
     1261 gtgccgtcaa cgcgcctaaa acgatctcag gcacgcgcag gtggccgagc gccagctcga
     1321 actcaacgac gacggtggcc tgcgcggcct gcagcgttcc gcttctgcgc agcgcgctgg
     1381 cctgttcacg ggtgcagcgg tggcgttgtc ggatgcgccg tccatcggga tctgctgggg
     1441 gccgttgatt gatcagcgga cgtcgtgagc gtgttgcgtg tcggggctgt cgacggcgcg
     1501 ggtggcagcc cagctggcga gcagtttgag tccgtcggcg gtaggggtgt gcgggtcagc
     1561 ggtgtagatg ttgaggcgca gctcgggctc ggaggggagc tccatggctt cgaagttgag
     1621 gtcgagttcg ccgacggcgg ggtggcggag tcgtttgcgg ccggtgcggt ggaacttgac
     1681 gttgtgtgag gcccagtgct ggcggaacag ttcgctttgg gtggagagtt cgccgacgag
     1741 ggtgatgagg tccttgtcgt gagggttgcg tcctgcttcg agccggagca tggcggcggc
     1801 gtcggtggcg atgcggtcgt agtcgatgaa gaactcttgg gcggccggat cgaggtagat
     1861 gaatctggtg gtgttggccg cgcgtttggt tgtcgcgccg gcaacggcgg agtccaggac
     1921 cggcgagtac agcgcgcggg cgagggggtt catcgcgagg atgtcgtggc gggcgttgcg
     1981 gatccaggcc ggcgcttcgg tgattgcgtc tagaacctgc tggatgaccg ggcgaaccgt
     2041 ggtcgggctg ggtcgggccc gacgggtcga ggttgcaccg gcggagcggg cgagcgcgat
     2101 caggtgttcg cgctcggctt cgtcgagttg gagcgcgttc gcgaccccgt cgaggacggc
     2161 ctcggaggca ccggcgaggt cgccgcgttc catccggatg tagtagtcga tgctgacccc
     2221 ggcgagtagc gcgacctctt cgcggcgcag gcctttcacc cggcggttgc cgccgtaggc
     2281 gggcagcccg gcctggtcgg gggtgatccg cgcccgccgg gaggcgagga attcgcggat
     2341 ctgcgctcga ttgtccatga cttcacggta ggtgaccacg gcggcgccag ggaggtaccg
     2401 ccgttaccta taagagcaga cactcccatg ggcgcgcgca cccgcgttga gtggttgtcg
     2461 tgagcacagt taccgagaca gctgacaatc ggcacatcga tcgtgagtcg ctgaaggtgc
     2521 tggcacgggc tgtgaccgcc tcgaacggtt ggggcgccga cgagctggcc acgatcgacc
     2581 gcacgggcga ggtcgacgtc gccacccggc gcggcgatgg cacgctgcgt gcggcccgca
     2641 tcgtgtggat cgtgcggcac ggcgacgccg cgtacgtccg ctccgtcaat ggcacgagcg
     2701 ccgcctggta ccggggcgtc cagacccgcc acgagggcga gctgggcgcg ggacgcttgc
     2761 ggcgggacgt ggccttcgtc gaggccggcg agcacgccgg cgacagcagc gggctggacg
     2821 acgccctgga tgccgcctac cg
//
LOCUS       DASSGQ010000327        16293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_480235,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000327 DASSGQ010000000
VERSION     DASSGQ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 16293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16293
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480235"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VFI55_14230"
     CDS             <1..691
                     /locus_tag="VFI55_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7075225.1"
                     /translation="DVALRGEIAISDAAAANVLLILAGSRDGSGLFAVKTDSPGVSVT
                     PERGIDPTRKQFRVALDDATAHRLATSSPDAMAALVDDVLIAAAADALGAARMVMDLA
                     VEYAKLRTQFGRVIGSFQAIQHLCVDMYETVELARSGVIHALWAADNADAQQRHLSAV
                     RAKAFAGRLASVGDIAIQVFGGIGYTWEHDAHLYLKRLLGWSAFLGSPDRYLTELGRT
                     LANPEKEGRPS"
     gene            688..1617
                     /locus_tag="VFI55_14235"
     CDS             688..1617
                     /locus_tag="VFI55_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7075226.1"
                     /translation="MIDFTDQVAIVTGAGRGLGRWYALELARRGAAVVVNDLGGTMHG
                     EGADTSIADQVVAEIEGAGGVAVASHDSVASPQGGEAIVAMAVERFGRLDAVVSNAGI
                     FNSVAFEELSVDDWRRMLSVHLDGGFYLSQPAYRVMKAQRYGRFVFISSSGGMFGQPM
                     EAHYAAAKAGLVGLTNVIAIEGAEHGILANAVLPFGYSRMVTETVGDPKALEEIGFLG
                     VIKPELVVPMVVFLASRACGFTHQNYSACAGRFARVFVGLGQGWLADHGRVPTADDIV
                     AHLDEVAATEPFTVPGSIFEEVFAVCEQLGVSG"
     gene            1826..3142
                     /locus_tag="VFI55_14240"
     CDS             1826..3142
                     /locus_tag="VFI55_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7075227.1"
                     /translation="MAKTESVPKGRPLAGGRVNHAGVADDSGTRRTEILQTAASLIAS
                     SGLRTSLQEIADAAGILPGSLYHHFESKEAILVELIRRYQDDLNRIGQSAQARLDEPD
                     SRPVSEKIIELGKAIANCAVQHGAALQMSFYEGPSADPQLITLTQQRPTAIQQAMLQT
                     LRAGRWSGYIKPDIDLPTLADRICQTMLQVGLAVMRHNSSPDQVAELLCRIILQGLAV
                     RPPTDAALDRSKALAAANDVIETWADESDAGPNDKAAHVRAVARTEFGRKGYEVTTIR
                     DIASAAGLGTGTVYRVIGSKDELLASIMQSFGKKVEAGWVGVLRSDATPVEKLDALSW
                     VNVNALDRFSDEFRIQLAWMRQSPPNTPNPGWLYATRLRQMKSLLSEGIRSGEITNDA
                     PSTTMLARCVIGLQWIPENILHAVGKRAALMHARDTVLRGVAVRTK"
     gene            3234..4076
                     /locus_tag="VFI55_14245"
     CDS             3234..4076
                     /locus_tag="VFI55_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7075228.1"
                     /translation="MTVMDRLRYDGKRALVVGGATGMGAAAAKSAAELGAEVIVMDYA
                     PVAYDVAKALQVDLRDPASIDATLEQVGGPVHAVFSAAGVAEGPDLMKINFIGHRYLI
                     ERLIDRGWLGSGSAICFISSVAGMGWENDLPLLQEFLAAPDFAAAQNWVSAHEAQGII
                     HYGFSKKAVNAYVATQGYPLLKKGIRINAICPGPTDTPLAQANADLWLTFAQDYRDET
                     GSKVHTPEQMGDVMCFLNSGAAFGINGITLLVDYGHTMASLTGAFAPGKPIIDLIMGR
                     VQLT"
     gene            4091..4747
                     /locus_tag="VFI55_14250"
     CDS             4091..4747
                     /locus_tag="VFI55_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019734651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET7075229.1"
                     /translation="MPRIVVIGGHGKVALLLARILTERGDAVSSVFRNPGHSADVAAT
                     GADPVVADIEQLDTNALAGLLAGHDAVVFSAGAGGGNPARTYAVDRDAAIRVIDAAAA
                     AGVKRFVMVSYFAAGPDHGVAQDDPFFPYAQAKAAADAHLCASGLDWTVLGPSRLTLD
                     APTGRIELGAAGTRIAKSEVSRSDVAAVVVAALADDSTIRRTIGFNNGDVPIAEALGA
                     "
     regulatory      4826..5023
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5161..7431
                     /gene="metE"
                     /locus_tag="VFI55_14255"
     CDS             5161..7431
                     /gene="metE"
                     /locus_tag="VFI55_14255"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HET7075230.1"
                     /translation="MTSQPFTATVTGSPRIGPKRELKRATEGYWAGRTSRTELESVAA
                     TLRSDMWADLAAAGLDSVPVNTFSYYDQMLDTAVLLGALPARAEQVSDDLDRYFAAAR
                     GNKDVAPLEMTKWFDTNYHYIVPEIGPATTFALNPDKVLFELKEALAQGIPARPVIIG
                     PVTFLLLSKGVDGGGAPIERLQELVPIYAELLSLLADNGAKWVQLDEPVLVTDISPDA
                     PALAEAVYNKLGAVSNRPAIFVATYFGDPGASLAGLARTPVEAIGVDLVYGADTAIAG
                     VPELANKTLVAGVVDGRNIWRTDLESALGKLATLLGSAGNVAVSTSCSTLHVPYLLEP
                     ETGLDDALRSWLAFGAEKVGEVVTLARALHEGRDSVADEIAASNAAVASRKSDPRLHN
                     GQVRARIDSIVASGAHRGDAAQRRASQDARLHLPPLPTTTIGSFPQTVEIRKARAALV
                     AGEIDEAEYTRRMQSEVAAVIKLQEELGLDVLVHGEPERNDMVQYFAEQLDGFFASKN
                     GWVQSYGSRCVRPPVLYGDVTRQQPMTVEWARYAQSLTDKPVKGMLTGPVTILAWSFV
                     RDDQPLADTAYQVALAIRDETVDLQSAGIAVIQVDEPALRELLPLRRADQDEYLRWAV
                     GSFRLATSGVADSTQIHTHLCYSEFGEVIGAIADLDADVTSIEAARSHMEVLDDLNSI
                     GFANSVGPGVYDIHSPRVPSTDEMAASLRAALKAVPAGRLWVNPDCGLKTRKTDEVTV
                     SLQNMVAAAQEVRAGA"
     gene            complement(7569..9305)
                     /locus_tag="VFI55_14260"
     CDS             complement(7569..9305)
                     /locus_tag="VFI55_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HET7075231.1"
                     /translation="MGFLKPELPQIDMTEWNKGTRSEKIRPMARHWAEVGFGTPVALH
                     LFYVVKILLYVLGGWLFASATKGIGGFTNVASWWSEPIVFEKVVLYTMLFEVIGLGCG
                     FGPLNNRFFPPMGSILYWMRFGTIRLPPWPDRVPWTKGTARKPVDVFLYAALLLTMLV
                     ALLSDGTGPIPELGTKVGLLPGSKIVIILLFLGVAGLRDKVIFLAARGEVYGTLTVTF
                     LFGGVDMIVAAKLVFLVIWMGAATSKLNKHFPFVISTMMSNNPLVWPRGLKRRFFEKF
                     PDDLRPGRLSRWLAHVSTAIEMLVPLVLFFSHGGWPTTVAATAMVCFHLGILLSIPMG
                     VPLEWNVFMIFGVLSLFVAHARLGLSDLKHPVPVAILFVVITGIVALGNLFPRKISFL
                     PGMRYYAGNWDTTLWCIKPSANEKIGKGIVAIASMPQAQLERFYGSPEAAQIPIYMGY
                     AFRGFNTHGRALFTLAHRAMAGQNEDDYVITDGERICSTAIGWNFGDGHMHNEQLIVA
                     MQQRCRFAPGEVRVVLLDAQPIHRQTQAYRLVDAATGEFEHGIVRVADMVTSQPWADD
                     IPVQVLSDEAAQ"
     gene            9430..9648
                     /locus_tag="VFI55_14265"
     CDS             9430..9648
                     /locus_tag="VFI55_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075232.1"
                     /translation="MSRHRFVVLAMPALAAIAVLAGGGVARADAPAPGDTCTVFHATT
                     HDVNGRTMWCNPTVTDAHNLMWQYGEPS"
     gene            complement(9814..11268)
                     /locus_tag="VFI55_14270"
     CDS             complement(9814..11268)
                     /locus_tag="VFI55_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075233.1"
                     /translation="MTVRFRGLLVVPLFLALVLALAPSAWADTYTDQVMGKFNAQTHV
                     VADSAASPPLRNPEQLNQQILGSRWTWSPAPPIWVAAVAANQSGVTTADAIHNAFLGR
                     DPAFSGIILVIDSRGYHVRAYNVPKAVADSVDPFMGQSAGDHRGDPYGATSEFVSKLA
                     NVNVSANGPAQTSPTVTANHSSSPWLWVLIIFGGALLVAGLIWFAVNRNRKRRKDTEA
                     RDQIKQQLIVAESEVSDLDNAVVSNSGADVSAESLKANSGLYDARKAYQAGDYSAARA
                     HLRVVQSAVARANRKLYPDRPAPDVDAVTAVPDEDRKQAVVRAKDPDTGQYVTINNNN
                     YSTTPQSGYPNYYGGGYYNGVFFYPGYYPYAFWGPGWGWAMTDVLLMDTLLDDRWDGA
                     YDRGFEAGRDSAWADSNVDSGQTASYDSQQGDVGFDGSFQGSGGGDTGFGGNGYSGGF
                     DSGGNFDFGGSDSGGGFDFGGSDFGNDSGGNFGF"
     gene            11588..12955
                     /locus_tag="VFI55_14275"
     CDS             11588..12955
                     /locus_tag="VFI55_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7075234.1"
                     /translation="MAEVDYCVVGAGFAGLTAALRLQRAGRSVALLEARDRVGGRTFT
                     QTRPDGTWVDRGGAWIGPGQDRIYALMNEFGVPEYKQHNDGDAMMIVDGKKHRYGGTI
                     PWTISPWAVTNLGLGLHAIEKMCKSIPREAPWEAKQAEEWDRISLGEWIEKNTMSAQA
                     RQMLDMALAGPYTSAASETSLLWVLLQMASGGGPTFVISGKGGSQDARPVGGMGAIYR
                     PMAAELADAVHLSQPVRQIAQDADGVTVRAHDLTVRARRVIVAIPLAIATSIDYEPML
                     PVDRAFLHQRMPSGAVFKISVVYDEPFWRADGLSGQSASPGSPATLTIDACTDTGKPG
                     IMCVITEGPAARRLGKLDEAERKAVVIRELVDRFGNKANAHLEFHEQNWTVERYSGGG
                     MISHAPTGVLTEYGYMLREPCGRVHWAGTESSATMCGWIDGAIRSGERAAAEVMSADT
                     AAVVG"
     gene            complement(12976..13260)
                     /locus_tag="VFI55_14280"
     CDS             complement(12976..13260)
                     /locus_tag="VFI55_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HET7075235.1"
                     /translation="MTINYQFGDVDSHGALIRAQAASLEAEHQAIVRDVLAAGDFWGG
                     AGSVACQEFITQLGRNFQVIYQQANAHGQKVQAAGSNMAGTDAAVGSSWA"
     gene            complement(13289..13585)
                     /locus_tag="VFI55_14285"
     CDS             complement(13289..13585)
                     /locus_tag="VFI55_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HET7075236.1"
                     /translation="MAIRFMTDPYAMRAMASRFSVHAHTVEDEARRMWASSQNISGAG
                     WSGAAAATSYDTMGQMNQAFRNIVSMLEGVRDGLVRDANHYEQQEQASQQVLSS"
     gene            complement(13683..14774)
                     /locus_tag="VFI55_14290"
     CDS             complement(13683..14774)
                     /locus_tag="VFI55_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009938502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPE family protein"
                     /protein_id="HET7075237.1"
                     /translation="MHFLVLPPEINSALMCAGAGSGPLFLAAAAWDGLGAELYASASS
                     FDSVITGLTAGPWSGAASVAMAAAAAPYASWLAASAAHAEGAAAQARMAAAAFDAART
                     ATVHPAAVTANRVSLVSLIATNFLGQNTPAIAATEFDYLQMWAQDVAAMVGYHAGATS
                     VAATMTPFAAPPVDLAGLASQVAGEASVAVQSLASGAPLQSLTSVAQIAMLPVSMLMS
                     PMTSLAQGANAGTAGLADAVSVPKFVDSTAAAMKPVGGGGAGLGGSVGASASLGNARL
                     VGAMSVPPTWQGSLPARLASSAMSGLGGETPYAAAAAGAQMGGMPMMPMPLGGLGGGG
                     MPGGMWGRGGASPHVVQSRPSVVPRTGVG"
     gene            15112..>16293
                     /locus_tag="VFI55_14295"
     CDS             15112..>16293
                     /locus_tag="VFI55_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HET7075238.1"
                     /translation="MSEIDVRADIGSWNGRDRGVSEFLPTGTVTLLLADFEPSTRLWE
                     TQPEQMTTTRLRLERAAAEIVAAHDGALVAKPGAGDSLTAAFAHATDAVACAVELQRA
                     ALAPIRPRVGVHTGEVQLDDAGDYAGPAPKRAARLRELAHGGQILLSGITRDLVVDQL
                     PADAWLTELGAHQLGDLPRPERVAQLCHPDLDVEFPPLRTTGGVVPHGLPVQLTRFVG
                     RVGLTNDVAKALSENRLVTLAGAGGVGKTRLAVQVAAQAAAEFDGNVWFVDLTPVTDP
                     AMAPVAAIRALGLPDQPGRSATDTLVRFLGDRRVLLVLDNCEHLLDVCAALTVSLLGG
                     CPQLTILTTSREPIGVAGELTWRVPPLSLADEAIELFVDRARLARPEFSIGVDDTPAV
                     RE"
BASE COUNT         2831 a         5468 c         5330 g         2664 t
ORIGIN      
        1 ggatgtcgcc ttgcgaggcg agatcgcaat atccgacgca gccgcggcga atgtcctgct
       61 gatacttgcc ggaagccgtg atggctccgg acttttcgcg gtgaagaccg actccccggg
      121 tgtttcggtg acacccgagc gcggtatcga cccgacccgc aagcagtttc gggtcgcact
      181 cgacgacgcg acagctcacc ggctggccac gtcgtcaccg gacgccatgg cagccttggt
      241 cgacgacgtg ctcatcgccg ccgccgccga cgcgctcggc gccgcacgga tggtcatgga
      301 tctggcggtc gaatacgcaa agttgagaac acaattcggt cgggtaatcg gctcgtttca
      361 ggcaatccag cacctgtgcg tcgacatgta cgagacggtc gagctggccc gcagcggggt
      421 catccacgcg ctgtgggccg ccgacaatgc cgatgcacag cagcggcatc tgtcagcggt
      481 gcgcgccaag gctttcgcgg ggcggctggc cagcgtgggc gacatagcga tccaggtgtt
      541 cggcggcatc ggctacacct gggagcacga cgctcacctc tacctcaaac gcctgctcgg
      601 ctggagcgca tttctcggct cgccggaccg ttacctcacc gaactcggta gaaccctggc
      661 gaacccggaa aaggaagggc ggccctcatg atcgatttca ccgaccaagt tgcgatcgtc
      721 accggagcgg gccgcggcct gggacgctgg tatgcgctgg agctggctcg ccgcggtgct
      781 gccgtggtcg tgaacgatct gggtggaacc atgcacggcg agggcgccga taccagcatc
      841 gcggatcagg tcgtggccga gatcgagggc gccggcggcg tcgccgtggc ctcccacgac
      901 tctgtcgcca gcccgcaagg cggagaggcg atcgttgcga tggccgtcga gcgcttcggc
      961 cggctcgacg ccgtggtgag caacgcgggc atcttcaaca gcgttgcgtt cgaagaactt
     1021 tccgtcgacg actggcggcg catgctcagc gtgcacctcg acggcgggtt ttatctgagc
     1081 caacccgcct accgcgtgat gaaggcgcag cgctatggcc ggttcgtgtt catctcctcc
     1141 tcgggaggaa tgttcggaca gccgatggag gcccactacg cggcggccaa agccggtctg
     1201 gtcgggttga cgaacgtgat cgcgatcgaa ggcgccgagc atggcatcct ggccaacgcc
     1261 gtgctcccgt tcggctattc gcggatggtg accgaaaccg tgggagaccc gaaagccctt
     1321 gaggaaatcg gctttttggg agtgatcaaa cccgagctgg tggtaccgat ggttgtcttc
     1381 ctggcgagcc gggcctgcgg gttcacccac cagaactact cggcgtgcgc gggtcgcttc
     1441 gcacgggtct tcgtcgggct cgggcagggc tggctcgccg accacggacg cgtcccgacc
     1501 gccgacgaca tcgtcgcgca cctggacgag gtagcggcga ccgagccgtt caccgtgcca
     1561 gggtcgatct tcgaggaagt ctttgccgtc tgcgagcagc tcggggtgag cggctgatca
     1621 cgccaagcgt tgtccacggc gcgcgcacgc gacgacgggg accgcccggc gctctagaat
     1681 ggcgccgtca gcgctttgtg cagcgctttg tgcagcgaat ttctttcgcc cggtctgata
     1741 gccttcaaca aagatttggt tcgccgggag gacgggtgcg catgttctgg aatccgcagc
     1801 cgtcgagggg cgcacaccga taaccatggc caaaaccgag tcggtgccaa agggccggcc
     1861 attggcgggc ggccgcgtga atcacgccgg ggtcgcagac gattcgggaa cgcgccgaac
     1921 cgaaatccta cagacggcgg catcgttgat cgcatcctcg ggattgcgga cgtcgttgca
     1981 ggagatcgcc gacgcggcag gcattctccc gggcagcctg tatcaccatt tcgaatccaa
     2041 agaggcgatc ctcgtcgagc tgatccgccg ctatcaggac gatctgaacc gtattgggca
     2101 atcggcccag gctcggttgg atgagcccga ttcgcgcccg gtttcggaga agatcatcga
     2161 gctgggaaag gcgatcgcca actgcgccgt gcaacacggg gcggcgctgc agatgtcgtt
     2221 ctatgagggg ccgagcgcag atccgcagct catcacgctg acacaacagc ggcccacggc
     2281 aattcagcag gcgatgctgc agacgctgcg cgcgggccga tggagcggct acatcaaacc
     2341 cgatatcgac cttcccacgc tggccgatcg catctgccag acaatgttgc aggtcgggct
     2401 tgccgttatg cgccacaact cttcgcccga ccaggtggcc gagctgctgt gccgaatcat
     2461 tttgcagggg ttggcagtta ggccacccac cgacgcggcc ttggatcgat ccaaggccct
     2521 tgccgccgca aacgacgtca tcgagacgtg ggccgacgaa agcgacgccg gtcccaacga
     2581 caaagcggcg cacgtccggg ccgtggcacg aacagagttc ggccgcaagg gctacgaagt
     2641 caccaccatc cgggacatcg cctccgcggc gggcctcggc accggaacgg tgtatcgcgt
     2701 gatcggatcc aaagacgaac tcctcgcctc gatcatgcag tccttcggga agaaggtcga
     2761 ggcgggctgg gtcggtgtgc tgcgttctga tgccacaccc gtcgagaagc ttgacgcact
     2821 gagctgggtg aacgtcaacg cactcgatcg tttttccgac gagttcagga tccagctcgc
     2881 ctggatgcgg cagtcgccac cgaacacgcc caacccgggc tggttgtatg ccacgcgact
     2941 ccgccagatg aaatcacttc tctccgaagg gatccggtcg ggagagatca caaacgatgc
     3001 cccgtcgacg acgatgctgg cacgatgcgt catcggcctg cagtggatac cggagaacat
     3061 tctccacgcg gtgggaaaac gtgcggcgct gatgcatgcc cgcgacaccg ttttgcgcgg
     3121 tgtcgccgta cgcaccaagt agcaacccgg ccgaggtggt acccaatgta gaaccaaata
     3181 actgttcctc atacagtagg gccattagta acggccgcgg aaagggagcc ccaatgactg
     3241 tgatggatcg gttgcggtac gacggcaagc gtgcgttggt tgtcggcggc gcgacgggca
     3301 tgggcgccgc ggcggccaag tcggcggccg agctcggcgc cgaggtcatc gtgatggact
     3361 acgcgccggt ggcctacgac gtcgccaagg ccctacaggt cgacctgcgc gacccggcct
     3421 cgatcgacgc cacgctcgag caggtgggcg gaccggtcca tgcggtgttc tccgccgccg
     3481 gggtcgccga gggaccggac ctgatgaaga tcaacttcat tgggcaccgc tatctcatcg
     3541 agcgcctgat cgacagaggc tggcttggct ccggctcggc catttgcttc atttcgtcgg
     3601 tggccggcat ggggtgggag aacgatctgc ccctgctgca ggagttcctg gccgcacccg
     3661 acttcgcggc ggcccagaac tgggtatcgg cgcacgaggc gcagggcatc atccactacg
     3721 gcttcagcaa gaaggcggtc aacgcgtacg tggcgacgca gggttatccg ctgctgaaga
     3781 agggcattcg catcaacgcg atctgccccg gcccgaccga cacgccgctg gcccaggcga
     3841 atgcggacct gtggctcacg ttcgcccagg actatcgcga cgagaccggc tccaaagtcc
     3901 acacgccgga gcagatgggc gacgtcatgt gcttcctcaa cagcggcgcc gccttcggca
     3961 tcaacgggat cacgctgctg gtggactatg ggcacacgat ggcgtcgctg accggggcgt
     4021 tcgcgccggg caagccgatc atcgacctga tcatgggccg cgtccagctc acctaaagta
     4081 ccgtcgtggc atgccacgca tcgtcgtcat cggcggccac ggcaaggtcg cgctgttgct
     4141 ggcccgcatc ctgaccgagc gcggcgacgc ggtgagttcg gtattccgca accccggcca
     4201 ttccgccgat gtggccgcca cgggcgctga cccggtggtg gccgatatcg agcaactcga
     4261 caccaacgcg ctggccggcc tgctggccgg gcacgacgcg gtcgtctttt ccgccggcgc
     4321 gggcgggggt aacccggcac ggacgtatgc cgtcgaccgc gacgcggcga tccgggtgat
     4381 cgatgccgcc gcggcggcgg gcgtgaaacg attcgtgatg gtgtcctact tcgcggccgg
     4441 tcccgatcac ggtgtagcgc aagatgatcc gttcttcccc tatgcgcagg ccaaggccgc
     4501 ggcagatgcc cacctatgcg ccagcggcct ggattggacg gtcctgggac cgagccggct
     4561 gaccctcgac gctccgaccg gtcggatcga attgggggct gcgggaacac gcatcgcaaa
     4621 aagtgaagtc tcgcgctcgg acgtcgcggc cgtcgtggtc gccgccctgg cagacgattc
     4681 gacgatccgc cgaaccatcg gattcaacaa tggcgatgtg ccgatcgccg aggcgctggg
     4741 agcctgaacc ggcgttttcc aggcgttcga gctacggagt agactgcacg acgcaactgg
     4801 tctgccgtcg ccgcgaacgc ggcgccggca tcgaacgagg catgttagac atgcgtcgca
     4861 agagggaacc cggtgagaat ccgggactgt cccgcagcgg tatgcaggaa cgaccgccgt
     4921 cacaaagcac tgggcccgaa cccccgggaa gcgacggcca ataggagcac cacgcggtgc
     4981 gcgcctgcga gtccgaagac ctgccagctg tgccgggcgc gccgcgtccg gcggcacatc
     5041 gcctcgtgga atgggcgttt ggctgaagcc gttgcggtta aacacctagg ggtgccaccg
     5101 caaccgggtc ggctgcatgt ccaccggcgg tgaccaccac gtcgattgaa ggacaagata
     5161 gtgacctctc aaccattcac cgccacggtt acgggctcgc cacgcatcgg cccgaaacgc
     5221 gaactcaagc gtgcgaccga gggctattgg gccgggcgca ccagccgcac ggaattggag
     5281 tccgtcgccg ccacgttgcg cagcgacatg tgggcggact tggccgccgc cggcctggac
     5341 tcggtaccgg tgaacacctt ctcctactac gaccagatgc tcgacaccgc ggtcctgctc
     5401 ggtgcgctgc cggctcgcgc cgagcaggtt tccgacgacc tggaccgcta ttttgctgcg
     5461 gcgcgcggca acaaagacgt cgcgccgctg gaaatgacca agtggttcga caccaactac
     5521 cactacatcg ttcccgagat cgggcccgca acgacgttcg cgctgaaccc ggacaaggtg
     5581 ctcttcgagc tgaaagaagc tctcgcgcag ggcattccgg cccggccggt gatcatcggt
     5641 ccggtcacgt tcctgctgct gagcaagggc gtcgacggcg ggggcgcacc gatcgagcgg
     5701 ctgcaggagc tcgtcccgat ctacgccgag ctgctgtcgc tgctcgccga taacggggcg
     5761 aagtgggtgc agctcgacga gccggtcttg gtgaccgaca tctcccccga cgcgcccgcc
     5821 ctggccgagg ccgtctacaa caagctgggc gcggtgagca atcggcccgc catcttcgtc
     5881 gccacctatt tcggcgaccc gggcgcgtcg ctggccgggc tggcccgcac gccggtcgag
     5941 gcgatcggcg tcgacctggt ttacggcgcc gacacggcga tcgcgggggt gcccgagctg
     6001 gccaacaaga ccctggtggc cggtgtcgtc gacgggcgca acatctggcg caccgacctg
     6061 gagtcggcgc tgggcaagct ggcgaccctg ctgggctcgg cgggcaacgt cgcggtctcg
     6121 acgtcctgct cgaccttgca cgtgccgtac ttgctggaac cggaaaccgg cctggacgac
     6181 gcgctgcgca gctggctggc cttcggggct gaaaaggtgg gcgaagtcgt cactctcgcc
     6241 cgcgcgttgc acgaaggacg cgactccgtc gccgacgaga tcgcggcatc caacgccgcc
     6301 gtggcctccc gcaagagcga cccgcggctg cacaacggcc aggtccgtgc ccgtatcgac
     6361 tcgatcgtgg cctccggtgc gcacaggggt gacgccgccc agcggcgcgc cagccaggac
     6421 gcgcggctgc atctgccgcc gctgccgacc acgaccatcg ggtcgtttcc gcagacagtc
     6481 gagatccgca aggcccgcgc cgcgctggtc gccggtgaga tcgacgaggc cgagtacacc
     6541 cggcggatgc agtccgaggt cgctgcggtg atcaagctgc aggaggaact cgggctggac
     6601 gtgctggtac acggtgagcc ggagcgcaac gacatggtgc agtacttcgc cgagcaactg
     6661 gacggtttct tcgccagcaa gaacggctgg gtgcagtcct acggcagccg ctgcgtacgg
     6721 ccgccggtcc tctacggtga cgtcacccgc cagcagccga tgacggtgga gtgggccagg
     6781 tacgcccagt cgctgaccga caagccggtg aagggcatgc taaccggacc ggtgacgatc
     6841 ctggcttggt cgttcgtccg cgacgaccag ccgctggcgg acaccgctta ccaggtagcg
     6901 ctggccatcc gcgacgagac ggtggatctg cagtccgccg gcatcgcggt cattcaggtt
     6961 gacgagcccg cgctgcgcga actgttgccg ttgcgtcgcg ccgatcagga cgagtacttg
     7021 cgttgggccg tcgggtcgtt ccggttggct acctccggcg tcgccgactc gacccagatc
     7081 cacacccacc tgtgctactc ggagttcggt gaggtgatcg gggccatcgc cgacttggac
     7141 gccgacgtga cgtccatcga ggcagcccgt tcgcacatgg aggtgctgga cgacctgaac
     7201 tcgatcggct tcgccaacag cgtgggcccc ggcgtctacg acattcactc gccgcgggtg
     7261 ccgagcaccg acgagatggc cgcatcgcta cgcgcggcgc tgaaagccgt tccggcgggc
     7321 cggttgtggg taaacccgga ctgcggcctg aagacccgca aaaccgacga ggtgacggtg
     7381 tcgctgcaga acatggtcgc cgcggcgcag gaagtgcggg caggggcgta gttctcgcgc
     7441 gagcgtaacg ccactgcgac tttcgaaccg aaatttcgcg gtggcgttac gctcggcgac
     7501 gatgcgcgcc gcgaagcggc gtgagaagga gccgggcaat ccaactcacg ctcggcgacg
     7561 atgcgcgcct attgggcagc ttcgtccgac agcacctgga ctggaatgtc gtccgcccag
     7621 ggctggctgg tcaccatgtc ggcgaccctg acgatgccgt gctcgaactc gccggtggcc
     7681 gcatccacca ggcgatacgc ctgggtctgc cggtggatcg gctgcgcgtc gagcagtacc
     7741 acgcgcactt caccgggtgc aaagcggcag cgctgctgca tggcgacgat cagttgctcg
     7801 ttgtgcatat ggccgtcacc gaagttccag ccgatcgcag tgctgcagat ccgctcgccg
     7861 tcggtgatca cgtagtcgtc ttcgttctgg cccgccatgg cccggtgcgc cagcgtgaac
     7921 agcgcccggc cgtgcgtgtt gaaaccgcgg aacgcgtatc ccatgtagat cgggatttgc
     7981 gccgcttccg ggctgccata gaagcgctcc agctgggcct gcggcatgct ggcgatcgcc
     8041 acgatgccct tgccgatctt ctcgttggcc gagggcttga tgcaccacag cgtggtgtcc
     8101 cagttgcccg cgtaataccg catgcccggc aggaacgaga tcttgcgcgg aaacaggttg
     8161 cccagcgcga cgattccggt gatgacgacg aacaggatcg ccaccggtac cgggtgtttg
     8221 aggtcgctca accccagccg agcgtgcgcc acgaacaacg acaacacgcc gaagatcatg
     8281 aacacgttcc actccagcgg cacccccatc ggaatggaca acagaattcc caagtggaag
     8341 cagaccattg cggtggcggc caccgtggtg ggccaaccgc cgtgagagaa gaacagcacc
     8401 aacggcacca gcatctcgat ggccgtactg acgtgagcca gccaccggga caaccgcccg
     8461 ggccgcagat cgtccgggaa cttctcgaag aagcgccgct tcagcccccg gggccacacc
     8521 agcgggttgt tggacatcat ggtggagatc acgaacggga agtgtttgtt gagctttgac
     8581 gtcgccgcgc ccatccagat caccaggaac accagtttgg ccgcgacgat catgtcgacc
     8641 ccgccgaaga ggaaggtcac cgtcagcgtc ccgtagacct cgccgcgggc ggccagaaag
     8701 atcaccttgt cacgcagccc ggccacgccg aggaatagca ggatgatcac gatcttcgac
     8761 cccggcagca acccgacctt ggtgccgagc tcgggtatcg ggccggtgcc gtcggacagc
     8821 agcgccacca gcatcgtcaa cagcagcgcc gcgtacagga agacatccac cggcttgcgg
     8881 gcggtgccct tggtccacgg gacgcgatcc ggccacggtg gcagccggat ggtgccgaac
     8941 cgcatccagt acaagatcga acccatcggg gggaagaacc tgttgttcag cgggccgaac
     9001 ccgcaaccca aaccgatcac ttcgaacagc atcgtgtaga gcacgacctt ctcgaacacg
     9061 atcggttccg accaccacga cgcgacgttg gtgaacccgc cgattccttt ggtggccgat
     9121 gcaaacagcc acccgcccag gacgtagagc aggatcttga cgacgtagaa caggtgcagc
     9181 gcgaccggcg tgccaaagcc cacctcggcc cagtgcctgg ccatcggacg tatcttctcg
     9241 ctgcgggtgc ccttgttcca ttcagtcatg tcgatttgtg gcagctcggg cttgaggaat
     9301 cccatggttt gtcacactag aagcgttctt cgcgagtgtg cccttgttcg ggcgaagaat
     9361 cttttgccgg cgagtctttc gccgaatacg gtgaacgcga gtcgacgcct acgcaaggag
     9421 aagtcgacca tgagcagaca ccggttcgtc gtgttggcga tgcccgctct ggcagcgatc
     9481 gccgtcctcg ccggaggcgg tgtcgcgcgg gccgacgcgc ccgcgccggg cgacacctgc
     9541 acggtgttcc atgcaaccac ccacgacgtc aacggccgga cgatgtggtg caacccgacc
     9601 gtgacggacg cccacaacct gatgtggcag tacggcgagc cctcgtaggt cgccctcgta
     9661 ggtcttcgcg ccaggccgta ttcactttcg cgccagccga tttccgtcaa cgacggcgtc
     9721 ggcgtcgaaa ggtctcaacc tgcgggcggc agatcccctg attcgtcctc gccgacggga
     9781 gtcggtacgg cgttgcgcct caacgctttt ccctcagaac ccgaagtttc cgccggagtc
     9841 gtttccgaag tcggacccgc cgaaatcgaa gccgccaccg gaatcggagc caccgaagtc
     9901 gaagttcccg ccgctgtcaa aaccgccgga gtagccgttg ccgccgaatc cggtgtcgcc
     9961 gccgcccgag ccctggaagc tcccgtcgaa cccgacatcc ccctgctggc tgtcgtagct
    10021 ggcggtttgc ccgctgtcaa cgttggagtc ggcccatgcc gaatctcggc cggcctcaaa
    10081 gccgcggtcg taggcgccgt cccaacggtc gtccaacaac gtgtccatca gcagcacatc
    10141 ggtcatggcc cagccccagc ccggacccca gaacgcgtac ggatagtagc cggggtagaa
    10201 gaagaccccg ttgtaatagc cgcccccgta gtagttcggg taaccggact gcggcgtcgt
    10261 gctgtagttg ttgttgttga tcgtcacgta ctgaccggtg tccgggtcct tggcccgcac
    10321 gaccgcctgc ttgcgatctt catccggcac cgcggtcacg gcgtccacgt ccggcgcggg
    10381 cctgtccggg tagagcttgc ggttggccct ggccacggcg gactgcacga ccctgaggtg
    10441 cgcacgcgcc gcgctgtaat ctcctgcctg gtacgccttg cgggcgtcgt acaggccgga
    10501 gttggctttc agcgactcgg cactgacgtc cgcaccgcta ttgctgacca ccgcgttatc
    10561 aaggtcgctg acctcagact cggcgacgat caactgctgt ttgatttggt ctcgtgcctc
    10621 ggtgtccttg cggcgcttac gattccggtt gacggcgaac cagatcaggc cggccaccag
    10681 cagcgcgcca ccgaaaatga tgagcaccca cagccacggc gaactgctgt ggttcgcggt
    10741 cacagtggga gaggtctgcg ccggtccatt ggccgaaacg ttcacgttgg ccagtttgct
    10801 gacgaattca ctggtcgctc cgtacgggtc accgcgatga tcgccggccg actgacccat
    10861 gaacgggtcc acgctgtccg cgaccgcctt cggcacgttg taggcgcgca cgtggtagcc
    10921 cctggaatcg atgaccagga tgatcccgct gaacgcggga tcgcgcccca ggaaggcgtt
    10981 gtggatggca tccgccgtgg tcacgccgct ctggttggcg gccaccgcgg cgacccagat
    11041 cggcggtgcg gggctccatg tccagcggct gccgaggatc tgctggttta gctgctcggg
    11101 attccgcaac ggcggactcg ccgccgaatc ggccaccaca tgcgtttgcg cgttgaactt
    11161 gcccatcacc tggtcggtgt aggtgtcggc ccacgcggac ggcgccagcg ccagtaccag
    11221 cgccaagaag agggggacca ccaggagccc gcggaatcgt actgtcactt ttctcctgcc
    11281 gttgggcttc gttttccttc agtagggccg cggctttcgc ctgcctcacc ggtgcacata
    11341 gcggccatgg ggcgccgcat ctccgctgcc ctcacttcgc tatgccggtc accgcgcgga
    11401 cgtttcgcac gctcatgacg atcgaaccac acgcagcgcc caacgcgacg agaagcacgg
    11461 agcaggcgcc tgttcgtcca cccgccggcc ggcttgaact gcccgcgaaa tcacggtgaa
    11521 cgcgccgccg ctctgcgggc gttcggtgta gtttgctgct ggggcggcac tagctgagga
    11581 gccgggcatg gctgaggtcg actattgcgt ggtcggtgcg ggattcgccg gtttgacggc
    11641 cgcgcttcgg ctgcagcggg ccggacgctc ggtggccttg ctggaggcgc gcgaccgggt
    11701 cggcggtcgg acgttcaccc agacgcgtcc ggacggaacg tgggtcgatc gtggcggggc
    11761 ctggatcggg ccgggccagg accggattta cgcgctgatg aatgagttcg gggtgccgga
    11821 atacaagcaa cacaacgacg gcgacgcgat gatgatcgtc gacggcaaga agcaccgcta
    11881 cggcggcacg atcccctgga cgattagccc gtgggccgtc accaacctcg gtctcggttt
    11941 gcacgcgata gagaaaatgt gcaaatcgat accgcgcgaa gccccgtggg aggccaagca
    12001 ggccgaggag tgggatcgga tcagcctggg cgagtggatc gaaaagaaca ccatgtccgc
    12061 gcaggcccgc cagatgctgg acatggcgct ggccgggccc tacacctcgg cagcctcaga
    12121 gacgtcgctg ctgtgggtgc tgctacagat ggcgtccggt ggtggtccga cctttgtgat
    12181 ctccggcaag ggcggttccc aggacgcccg tcccgtcggc gggatgggcg ccatctaccg
    12241 cccgatggca gcggaattgg cagacgcagt gcacctttcg cagccggtcc ggcagatcgc
    12301 ccaggacgcg gacggtgtga cggtccgcgc gcacgatctg acggtacggg cgcgccgggt
    12361 catcgtggcg atcccgctcg cgatcgccac ctcgatcgac tacgagccga tgctgccggt
    12421 ggaccgcgcg tttctgcacc agcgcatgcc aagcggagcg gtcttcaaga tttccgtcgt
    12481 ctacgacgag ccgttctggc gcgcggacgg actgtccggc cagtccgcct cgcccggatc
    12541 cccggccacg ctgacgatcg acgcctgcac ggacaccggg aagccgggga tcatgtgcgt
    12601 catcacggaa ggacccgcag cacgccggct gggaaagctg gacgaggccg aacgcaaagc
    12661 ggtggtgatc cgcgaactcg tcgatcggtt cggcaacaag gccaatgcgc acctggaatt
    12721 ccacgagcag aactggaccg tggagcgcta ttctggtggc ggcatgatca gccacgcccc
    12781 gaccggcgtg ctgaccgaat acgggtacat gctgcgtgag ccctgcgggc gtgtgcactg
    12841 ggccgggacg gagagctcgg ccaccatgtg cgggtggatc gacggtgcga ttcgctccgg
    12901 tgagcgcgcc gcggccgagg tgatgtcggc cgacacggcg gcggtggtcg gttgagctct
    12961 gcgatcggcg actgatcacg cccagctgga acccaccgcg gcatcggtgc cggccatatt
    13021 gctgccggcg gcctgcacct tctgtccgtg agcattggct tgctggtaaa tcacctgaaa
    13081 attgcggccc aactgcgtga tgaactcctg gcaggccacc gaaccggcgc caccccagaa
    13141 atcccctgct gccaacacat cacgcacgat cgcctgatgc tcggcctcca acgacgccgc
    13201 ctgagcccga atcaaggcac catgagagtc cacatcaccg aactggtaat taatcgtcac
    13261 cgcgaatcct cctgactgtg gaatagcact agctgctcaa aacctgttgc gacgcctgct
    13321 cttgctgctc gtagtgattg gcgtcgcgca ccaacccgtc ccgcacccct tcgagcatgc
    13381 tcacaatgtt gcgaaacgcc tggttcatct gacccatcgt gtcgtaggac gtggccgcag
    13441 cggccccact ccagccggca cctgagatgt tctgcgacga cgcccacatg cgacgcgcct
    13501 cgtcctcaac cgtgtgcgcg tgcaccgaaa agcgactcgc catcgcccgc atcgcgtacg
    13561 gatccgtcat aaatcgaata gccacaacgt gttcctttca tgaaacgaat gaattcctga
    13621 tcatgagccg ccaggtgtcc ccggccgcgg cggtccgaag agctcgaccg atcacccccg
    13681 ccctatccga ccccggtccg cggcaccacg ctgggccgcg actgcaccac gtgcggactc
    13741 gccccgccgc ggccccacat cccgccgggc atcccgccgc cgcccagacc acccagcggc
    13801 atcggcatca tcggcatgcc gcccatctgc gccccggccg ccgcagctgc gtaaggcgtt
    13861 tcgccgccca accccgacat cgccgaactg gccagccggg ccggcaacga cccttgccag
    13921 gtcggcggca ccgacatcgc cccgaccagc cgcgcattgc ccaggctcgc cgacgcgccc
    13981 actgagccgc cgagccccgc accgccaccg cccaccggct tcatcgccgc agcggtgctg
    14041 tcgacgaact tcgggaccga aaccgcatca gccaacccgg ccgtgcccgc gttcgcgccc
    14101 tgcgccaacg acgtcatcgg agacatcagc atgctgaccg gcaacatcgc gatctgcgcc
    14161 acagacgtca acgactgcag cggcgcgccg gaagccagcg actgcaccgc caccgacgcc
    14221 tccccggcga cctgcgacgc caatcccgcc agatcgaccg gcggtgccgc gaacggcgtc
    14281 atcgtcgcgg ccacggacgt cgccccggcg tggtacccga ccatcgcggc cacgtcctgg
    14341 gcccacatct gcaggtagtc gaactcggtg gccgcgatcg ctggagtgtt ttgacccaga
    14401 aagttcgtcg caatcagcga caccaacgag acccgattcg cggtcaccgc cgccggatgc
    14461 accgtcgccg tccgcgccgc atcgaacgcc gccgccgcca tccgcgcctg cgccgccgcc
    14521 ccctccgcat gcgccgccga cgccgccaac cacgacgcat aaggcgccgc cgctgcggcc
    14581 atcgctaccg acgccgcacc cgaccagggc cccgcggtca acccggtaat caccgaatcg
    14641 aacgacgaag ccgacgcgta caactccgcg cccaacccgt cccaagccgc cgccgccaga
    14701 aacagcggcc ccgaacccgc cccggcacac atcagcgccg aatttatttc cggcggtaat
    14761 accaggaaat gcattgtccg tcccttctgg accggcggcc ggcacaccgt atggcggagc
    14821 cgtgctccgc gctccgttgg cgtcgggtga tcggtgatag cgcagcgaat tgcgatcccc
    14881 ggccccggcg tctgtaggaa acgagtcaaa tattggcggc gggacgtcga actaacgtcg
    14941 gtcggatcgc gtagatactc agcatgaggg gtcgtatttg cggtgaggtg cggggtcgta
    15001 gacccggcca gggcaaagcg tcagcccgta ttcccgggcc ggcgcgggtc tgctccgcca
    15061 aacccatgac ctcgacggtt ttcggccggt atcggaccct tggtggcgtg catgagcgaa
    15121 attgatgtac gcgcggatat cgggtcatgg aacgggcgtg accgtggcgt gagcgagttt
    15181 ctgcccaccg gaacggtgac cctgctgcta gccgatttcg agccctccac ccggctgtgg
    15241 gagacccagc cggagcaaat gacgaccacg cgtcttcggc tcgaacgtgc ggccgccgag
    15301 attgttgccg cccacgacgg cgcgctggtt gccaagccgg gcgcgggcga cagcttgacg
    15361 gccgctttcg cgcacgcgac cgacgccgtg gcgtgtgctg tggagttgca gcgggcagcg
    15421 ctggcgccga tccggccgcg cgtcggcgtg cacaccggcg aggtgcagct ggatgacgcg
    15481 ggcgattacg ccggtccggc gcccaagcgg gcggcccggc tgcgcgagct ggcgcatggc
    15541 ggccagattc tgctgtcggg catcacgcgc gacctggtgg tcgaccaatt accggccgac
    15601 gcctggctga ccgagctggg cgcccatcag ctcggcgacc tcccccgccc ggaacgggtg
    15661 gcccaactgt gtcatcccga cctcgacgtc gagttcccgc cgctgcgcac caccggcggc
    15721 gttgttccgc atggccttcc ggtgcagctg acgcggttcg tgggtcgcgt cggcctgacg
    15781 aacgacgtgg ccaaggctct gtccgagaac cggctggtga ccttggccgg cgcgggcggt
    15841 gtcggaaaga cgcggttggc ggtgcaggtc gcggcccaag cggcagccga attcgacggc
    15901 aacgtgtggt ttgtggatct gacaccggtc accgatcccg cgatggcgcc ggtcgcggcg
    15961 atccgggcgc tcggcctccc cgaccagccc ggccgctccg cgacggacac gctcgtgcgg
    16021 ttcctcggtg atcggcgggt actcctggta ctggacaact gcgagcacct gctcgacgtg
    16081 tgcgccgcac tcaccgtctc cctgctgggc ggctgtccgc agttgacgat cctgacgacc
    16141 agccgtgagc cgatcggggt ggccggcgag ttgacctggc gggtgccacc gctgtcgctg
    16201 gccgacgaag cgatcgaact cttcgtcgac cgggcgcggc tggcccggcc cgagttcagc
    16261 atcggcgttg acgacacccc tgccgtgcgc gag
//
LOCUS       DASSGQ010000328         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_631720,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000328 DASSGQ010000000
VERSION     DASSGQ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631720"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VFI55_14300"
     CDS             complement(<1..181)
                     /locus_tag="VFI55_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018178846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7075239.1"
                     /translation="MFEMSYRRLGKSGLAVSVVGLGCNNFGRKLDADGSRAVVDAALD
                     VGMNLFDTADIYGTPH"
     gene            417..1997
                     /locus_tag="VFI55_14305"
     CDS             417..1997
                     /locus_tag="VFI55_14305"
                     /inference="COORDINATES: protein motif:HMM:NF027798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,3-glucanase family protein"
                     /protein_id="HET7075240.1"
                     /translation="MSALAALAMAFPAAAFVSSPAQAVGPGLLPVTVTNNTGRGDAVY
                     LYVIGINLGTGRLGYVNQGGGFTPWPAGQIPPSPAPDVAIGGPGNGGSTTIQFPRGFS
                     GRVYFSLGEKLKFFLTPDGLVQPAPWANGDPNQNILFDWSEFTYNDAGLWLNSSQVDM
                     FAVPHAVTVTGSSGVTKKTGELLNNGRDNVINGIRSQSGWGNTVYTRSDGTVLRVLAP
                     GKAAGAGLLSANYLDSYISSAWNAYTGKTLTVVPFGDQPNTKFFGRTSGNVMNFTNTS
                     GQQVASFNRPSSASVWGCDGDLPAPNDLVVGPISRTLCAALNRGTIGTIDTQPSLNAA
                     DFYKNNPTNQYAKIIHANMADGKAYAFAFDDVGNFESLVNDGDPRSAGIILTPFGAGG
                     PSNPPPPSGVPVISNWNGKCIDVPSSNFSDSVPLQMWNCNGTNAQKWTFAGGALQSQN
                     NKCMDVAGGATANGAVVQLYTCNGTGAQQFVLNAASDLVNPQANKCVDIKDWNGNEGA
                     RLQLWDCHGGANQKWHKG"
BASE COUNT          372 a          704 c          637 g          294 t
ORIGIN      
        1 cgtgcggggt gccgtagatg tcggcggtgt cgaacaggtt catccccacg tccagcgcag
       61 catccacgac ggcccgggag ccgtcggcgt ccagtttgcg gccaaagttg ttacagccca
      121 gaccgacgac cgagaccgcg aggccggatt tgccgagccg gcggtagctc atttcaaaca
      181 tgatccgagc gtatgccgcc ctcgtgggga ggcccggaac gcccgtgccg gaggcaggcc
      241 ggaaacaatc tggcaataca tcagccgtta tctattgaca caccatgttc atcaccggat
      301 tctgtgagag cgctctctat ggccccgggg caggccggcg agcggcgcgc cacgcacacc
      361 catccgtgcc ctccaacgga attgaggtgc tccccgtgcg tacccgaaga agggccgtga
      421 gcgcgctcgc cgcgctcgcc atggccttcc ctgcggccgc gttcgtctca tccccggcgc
      481 aggcggtcgg cccgggcctg ctgcccgtta ccgtcaccaa caacacgggc cgcggcgatg
      541 cggtctatct gtacgtcatc ggcatcaacc tgggcaccgg ccggctcggc tatgtgaacc
      601 agggcggcgg gttcacgccc tggccggccg ggcagatccc gccgtcgccg gcgcccgacg
      661 tggcgatcgg cggtccgggc aacggcggca gcacgacgat ccagttcccg cgcggcttct
      721 ccggccgggt ctacttctcg ctgggcgaga agctcaagtt cttcctcacc ccggacggcc
      781 tggtgcagcc ggcgccctgg gccaacggcg atccgaacca gaacatcctc ttcgactgga
      841 gcgagttcac ctacaacgac gccggcctgt ggctgaacag ctcgcaggtg gacatgttcg
      901 cggtgccgca cgcggtgacc gtaaccggca gcagcggcgt cacgaagaag accggcgagc
      961 tgctcaacaa cggccgggac aacgtgatca acgggatacg ctcccagtcc ggctggggca
     1021 acaccgtcta cacccgttcc gacgggacgg tgctgcgcgt gctcgcaccc ggcaaggcgg
     1081 ccggggcggg cctgctcagc gccaactacc tggactcgta catcagctcg gcgtggaacg
     1141 cgtacaccgg caagacgctc accgtcgttc cgttcggtga ccagccgaac accaagttct
     1201 tcggacggac gtccggcaac gtcatgaact tcaccaatac ctccggccag caggtagcgt
     1261 cgttcaaccg gcccagcagc gccagcgtct ggggctgcga cggcgatctg cccgcgccca
     1321 acgacctggt cgtcggtccg atctcgcgga cgctgtgcgc cgcgctgaac cgcggcacga
     1381 tcggcaccat cgacacccag ccgagcctga acgccgccga cttctacaag aacaacccca
     1441 ccaaccagta cgccaagatc atccacgcga acatggcgga cggtaaggcg tacgcgttcg
     1501 cgttcgacga cgtgggcaac ttcgagtcgc tggtcaacga cggcgacccg cgctcggccg
     1561 ggatcatcct cacgccgttc ggtgccggcg gcccatcgaa cccgccgccg cccagtggcg
     1621 tgccggtgat cagcaactgg aacggcaagt gcatcgacgt gcccagctcc aacttctcgg
     1681 acagcgtgcc gttgcagatg tggaactgca acgggaccaa cgcccagaag tggacgttcg
     1741 cgggtggcgc actgcagtca cagaacaaca agtgtatgga cgtcgccggc ggtgcgaccg
     1801 cgaacggcgc ggtcgtgcag ctctacacct gcaacggcac gggcgcccag cagttcgtcc
     1861 tgaacgccgc gagcgacctg gtgaacccac aggccaacaa gtgtgtcgac atcaaggact
     1921 ggaacggaaa cgaaggcgct cgcctccagc tgtgggactg ccacggcggg gccaaccaga
     1981 agtggcacaa gggctgaccc gtccccg
//
LOCUS       DASSGQ010000329        24292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_995306,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000329 DASSGQ010000000
VERSION     DASSGQ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 24292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24292
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995306"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFI55_14310"
     CDS             <1..345
                     /locus_tag="VFI55_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7075241.1"
                     /translation="GLDSLATLRPSDDICTELGSLLADAEALRATGYRALGNELAGRP
                     SPEADILKLLGSVTLQRVWELTAVARGPQSASDADLLFERQDAMAATIYGGTSEVQRN
                     IIAERLLGLPKG"
     gene            342..1499
                     /locus_tag="VFI55_14315"
     CDS             342..1499
                     /locus_tag="VFI55_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7075242.1"
                     /translation="MTRMDYELGEDAGQLRNHLRELISHHIPADFLGACTDDPRDLAT
                     TETFCKLLAAEGLLALAWPKEHGGGGGSIWQQTVLREEMWAQHEPRGPQYMGINWVGP
                     ALMHYGTAEQKARHLSAIAAGDVIWCQGFSEPEAGTDLASLRTRALPDGDGWRITGQK
                     VWTSYARMASWCVLAACTDPDAPKHKRLTLFLLPMDRPGFTVRPIPSMLGPHHLNEMF
                     LDGVQAFRGDALGEPGDGWRVMREALAFERVGIARYARCESLLDRMITQLGDDWDRLP
                     ETIRARWVRALVDLRVARLLAYRAISLQDDPSAGAAASAARIATTTCDQQVAELLFDV
                     LGPAALDSGSSAPLHGAIEDHWRYAQAATVASGTIEVQRMLVARDVLGGHR"
     gene            1496..2431
                     /locus_tag="VFI55_14320"
     CDS             1496..2431
                     /locus_tag="VFI55_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7075243.1"
                     /translation="MKTVLPQDISDFAAVAAKRLARLGGPPAALRAETDDGVRAAARI
                     ALSDVGAFELDVRSAPDDLLAAAVLCQAAGATALPYPLVEELLAIEGARLALVNPEAP
                     RIDHGDLPGAWTAADLDGIRYHPQPASRTSAKLGPFLVPATLSAPAGSVPAADVNLHL
                     ILGSWWILGTMQQSLHIVTEHVRARIQFGKPLADFQAVRFAVADAAVAVRGLHELAKY
                     TVCRPKSLPARAHSADALVLRYKAADTARQVLRTSHQLLGALGFCDESDISVLDRHTQ
                     PLIRLPLSAEALALRLIPDVRGGQLETLFSGPVSA"
     gene            2428..3939
                     /locus_tag="VFI55_14325"
     CDS             2428..3939
                     /locus_tag="VFI55_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HET7075244.1"
                     /translation="MSRAGSVDAGVTSNPLEEGIPFGTKLAELAEQRRNDAAVTIVAL
                     DGTAQSLTFAELDARANQWGRALAAHRAETGSLVALAIPNSQHLVLATLGCWKIGAVP
                     IPMHWDLPDWERNRVRDVIDPAVVVDENSRWELDARAARESESPLPTAVSPTANGICS
                     SGSTGVPKVILNLAPSLWTDQQGEPFLSNWIPVAKPQTIMVPAPMYHTNGFATFLMLL
                     SGDHLVVLEKFNAALALDVIERFRITNFTATPTMLSRIAARPDVRQRNLSSVVFILQG
                     AAVMPPSLLHTWFELLSPEQIVTAYGMTENLGLTALRGDEWLAHPGSVGRGFRDTEIR
                     ILDAEKKPVGPGEHGDVYLRAPMSAGYRYLGGAPPLPSTDDGFRSAGDVGCLDEDGYL
                     HIVDRRVDMIVTGGANVFPAEVESALAGHPGIADVVVIGLSDPRWGRRVHAVVQLTDA
                     DGRAPLTEQQVIGYAKSRLAGYKVPKTVEFVAAIPRTAATKVNRSAMIAARGG"
     gene            4655..5842
                     /locus_tag="VFI55_14330"
     CDS             4655..5842
                     /locus_tag="VFI55_14330"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7075245.1"
                     /translation="MTSPPMHFKESPFFAADRSAYWRELRGLGPVVKIDVGNPMLRGY
                     YLTARDDVRAALLDPDTFASPPKTFNVFLFGELLPQVPLSCGTRSEHARFGRILHPLF
                     SPKGLAPFAPALRARATMVVDAVAAKGQCDAVDLADTYACQALVTVCGLPADDPRAVP
                     LTRAAVVGDADGTAHLALINWLLSGLNEGTRNPERPPGVLWPLLEGLDGDKDFPLSPV
                     EVVAVILLLFSVAGIEMAASAISFMLLHLGRDPRLQAQLREDPAQIPAFIEEVLRLET
                     PGPAIPRITTREVEIGGVTIPAGTAVWLALEAAGRENGGDEIATASNGEIQRQRHWAF
                     GVGMHRCLGMHLARLEIAAFVGEWLSRIPQFELEPGFTPAIVHKPTGVTHLGSLKLRW
                     EAR"
     gene            5948..6979
                     /locus_tag="VFI55_14335"
     CDS             5948..6979
                     /locus_tag="VFI55_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HET7075246.1"
                     /translation="MADPGEEAEVTRADRFIKTAVAILGETGRTDFTVQEVVARSKTS
                     LRAFYQHFSSKDELLLALFDRTIAQSAQSWRAETAGLDSTAALKLVIDRISQQPESST
                     QDSLNRALSLYNQHLAETRPREYARVLSPLHHLIRDIVGQGITEGVFNPGLDVGAAAA
                     IAMQTMVGAQRLHWLGSELNGTPVDAGQLYDFCSRALGIRDTDDESAAPSLAELFAQI
                     GMRPGTRDGQFAMTMPVSPAVVNTSGALQGGLIATLVDVAGGQFGLDYLQPGTTMTTA
                     DLFVRYLRPIRRGPALAVPRMLRTGRRAMVMQVDIYGDGDDELAATATVNFAIINGDT
                     PTVGLGAIT"
     gene            7070..8263
                     /locus_tag="VFI55_14340"
     CDS             7070..8263
                     /locus_tag="VFI55_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7075247.1"
                     /translation="MPSRELSFPVFDADNHMYEPQEALTKFLPDKRKHVIDYVQVRGR
                     TKIVVRGHISEYIPNPTFEKVAKPGAQEDYFRHGAQGKTYREILGEPMKAIPAFREPG
                     PRLEVMDELGIDYALMFPTLASLVEERMKDDPEMTHDVIHALNEWMHETWTFNYQERI
                     FATPVITLPIVDRALEELQWCLERGARTVLVRPAPVPGYRGSRSFGLEEFDPFWQACI
                     KAEIPVSMHASDSGYSEFANVWEPGDEFLPFKPTAFRSFAMGHRPIMDAMAALVCHGA
                     LSRNPELRILSIENGADWVPDLFKGLKGVYKKMPQSFTEDPVEAFKRCVYITPFWEDR
                     FTEIVTMVGTDRVLFGSDWPHPEGLKDPISFVDELADFEEADVAKIMGGNLMKLMKVA
                     KPVSA"
     gene            8495..10480
                     /locus_tag="VFI55_14345"
     CDS             8495..10480
                     /locus_tag="VFI55_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET7075248.1"
                     /translation="MVADEDWDKTVGAADEVRRIFETVPAMLVGLDGPDHRFVAVNAA
                     YRAFNPTGNPVGKLAREVFPELESQQIFLMLDRVYETAEPQSGSEWRLQADYDGSGVQ
                     ERFFDFVVTPRRRQDGSVEGVQIMFDDVTNRVQARLAAEARMQEMSERYRSVRDSATV
                     MQQALLAPSVPVVPGADIAAEYLVAAEDTAAGGDWFDAIALGDRLVLIVGDVVGHGVE
                     AAAVMSQLRTALRMQISSGHTIVEALEAVDRFHEHVPGSKSATVCVGSLDFATGEFQY
                     CTAGHPPPLLVGADANSRYAEPSGAGPLGSGTGFPVRTEVLGVGDSVLLYSDGLIERP
                     GRPLGASTAEFADLAASVAGGRGGFVIEAARRPIDRICSETLELLVRSTGYNDDVTLL
                     GAQRRTPPRPLHLTLGATIDTARTVRARLREWLSEIGADADDTCDVVHAVSEFVENAV
                     EHGYATKDAPGVSNTIVLEAALGGDGNLRASVIDQGSWKDYREGERGRGRGLAMAEAL
                     VSEAHITRSAGGTTASLTHRLSRQANFVTDTTFSRVTQQRLVNPEFVSMVSSDGPVGR
                     IVVSGDVDSTTASTLDRQIAVESRSGIAPLTIDLSGVTHLGSAGVSALVAARDRARKQ
                     GAECVLIAPPGSPAHHVLSLVQMPVTGGDVENVFVDE"
     gene            complement(10498..11730)
                     /locus_tag="VFI55_14350"
     CDS             complement(10498..11730)
                     /locus_tag="VFI55_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7075249.1"
                     /translation="MPTESVAEFAFRARVWLADNMPAIDHESPPPAPRDNERSWKRAR
                     ELQRRLYDGGFAGICFPSDYGGLGLDYEYQKAFDSESLDYEMPLILNTPTFTICCATL
                     LDTGTEDQKKRHIAAALRGDEVLVQLLSEPSGGSDLAGVITRAERRGDRWVINGAKTW
                     STSAFAADYGLCLARTNWDAPKHEGLTMFLVPIAHRGITLRRITMLSGSTEFCEEFLD
                     EVDVGDDAVVGGVNGGWAVASRQLYHERRAVGQGSEFASGSGSEGGTAKPVDYVALAE
                     MTGQADNERVHEMAGRVLVHRAVGEQLIDHVYRNVRDGVLPPAAGTLIRLFHSETVTL
                     DMDTALAIAGAAGVVGEIGEGLQTGLRYLSRQNVAIGGGTTEMARNVIGERVLGLPRE
                     YAADRGVPFNQVRHGQTR"
     gene            complement(11730..12890)
                     /locus_tag="VFI55_14355"
     CDS             complement(11730..12890)
                     /locus_tag="VFI55_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7075250.1"
                     /translation="MTAVDSPEKILFASTTQAFLHKEAPLRRVRELHAEGVSFDPAWW
                     RRAAELGWTALLVPEELGGGSVSGSGVEDLAMVAEQLGKTVAPGPLYPVSVVLAALVD
                     CADPQSHVATIESLVSGDTVASWAVSEPGRGWAPLDPSVTATRTETGFRIDGTKDRVE
                     AGAQSAVLLVVARCGQAAEEVRQFLVPTDAPGVRIEPQRSVDLVKQYARVQFDGVQVE
                     PSAVVGGAGGTATLIERQGQIAQVLQCAEVVGILQTVFDFTVQWALDRHTFGRPLASY
                     QALKHGFADMKLWLEACRATTAAAVADVAARSPAAELSASIAKSYVGEMAGRIVQACV
                     QMHGGIGVTWEHDLHLFLRRVTLYRSMFGTPEEHNLRVYALEKAARSMTTAR"
     gene            13078..13923
                     /locus_tag="VFI55_14360"
     CDS             13078..13923
                     /locus_tag="VFI55_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003407157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075251.1"
                     /translation="MAETNEPAAGSAPPESGAKALIEEAEAEAAAAEALAAAAKARAR
                     AARLRRQALGVPDPAEDYDADRDHDEDYDEDYDEDEDYEAADEHYPEDYDEEYAEDYD
                     ESGAEGDDETVTEQARWRRRMPTIAAVAAIVIICAFVGLSVYMVFQHRDATKHQQRET
                     AFVGGAKQGVLNMISLDFNKAKEDVQRVIDSSTGQFKDDFQKRANDLITVVTQSKAVT
                     EGTVNAAAIESMNGNSGVVLVSATSQVTNSPPGKDEPPKVWRLRVTVTDVGGQYKMSK
                     VEYVP"
     gene            13920..14570
                     /locus_tag="VFI55_14365"
     CDS             13920..14570
                     /locus_tag="VFI55_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075252.1"
                     /translation="MTDEMRDTTAVAADDDSDASQATTEADGSTGEAPGGVAEAESPE
                     PLKRPRFSLPPRLRKFAPVILAVLLLISGGVATWLYFKQYRPDQQTDASVAHAVVSAA
                     SDGTVALLSYSSDSLDKDFATARSHLAGDFLSYYEQFTQQSVAPVAKQKSMKTTAHVS
                     GAAVSELHPDSAVVLVFVDQSTTTKDSPQPTLALSSVLVHMTRINGTWLITKFNPV"
     gene            complement(14575..15534)
                     /locus_tag="VFI55_14370"
     CDS             complement(14575..15534)
                     /locus_tag="VFI55_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7075253.1"
                     /translation="MTQTNLTRPQGFNRIDPVLREAATELGVVEFRSETLPAEREHAN
                     LLAARRAAAADTDGVSVESRSIAGPDGRQLDIRLYRGRTESPAPMVVYAHGGGFVTGN
                     LDTDHAECVELARAGSCLLVSVDYRLAPDNPCPAALADVAAALRYAVENCADLDADAD
                     RIAVMGRDAGAALVAGLSQRMFDEEGPQILVQILHQPMLDSDATPSRREFQRTPGLNG
                     PAVSRAWNHYLGHSTARGQHVPAHRANLEGLPPTFISCSEIDPCRDEAIDYANRLLHA
                     YVHTELHVIAATFNGFDSLVPDWVVSQENRALHAQTLRRVFAM"
     gene            complement(15549..16697)
                     /locus_tag="VFI55_14375"
     CDS             complement(15549..16697)
                     /locus_tag="VFI55_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7075254.1"
                     /translation="MTTIERIDVATGQEGDIAVTIVDTDVHPLPVSVDVLKSYAPAEW
                     VDKIWPTGNAVTPVPHFYDTPDSYKTMSLRLDAAPPNGGFAGSDPDFAAKQLLLDAGV
                     SIAMLEPMCDAQLPQAEHVLKSTYNDWLADVWLDRHNAHGRWRGSVSVSAQAPEQAAR
                     EVQRWAGHPYMAQVLMTPQTRGIPFGNPHFDPLYEAAARNRLPVATHLMGQTPFELIP
                     LYPVGNPAHWHDFFASWPLLYVSHLMSLVFDGAFDRHPDLRVVFVEGGFTWAMPVMSR
                     MDRIWEARRGDLPHVRRRPSEYVREHVRFTTQPLEDTDTMGFREYLEMMDLGENLMFS
                     TDYPHWSYDSPTYAINRFPADQRERIMRGNATALYGLPATVKALPGQR"
     gene            complement(16697..17770)
                     /locus_tag="VFI55_14380"
     CDS             complement(16697..17770)
                     /locus_tag="VFI55_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7075255.1"
                     /translation="MSPITPIIDTCVQPHFRYNAEIRRYLPPAHQLRSIPDVEQQWYQ
                     APGGDYRQDLYGEHYPGSDPETVSRQLFDDTGSHLRVAYAILNPLTRGNIADYLLNTQ
                     ICAAVNDWLLDRWLEPDATNRFRGTIRVNPEDPKGAVAEIERLAAHPKLVQVGVPMQS
                     REPYGKPVFEPIWEAAAAHGLPVAVHINGGNGVDHPPTFAGHAHTYPGYAAFMPLNYF
                     VHLATLIVEGVFGRHPDLKFVFADGGYDILTPLMWRLDTFWLSMRDQTPWVDRYPSEY
                     LPGHVRFCWSAFDGPMQAGDMRRWLDFTAKSDLLMYGSSYPHWSTSAPEVAASELRGL
                     DEAKRDNVFWRNASELYGLKERV"
     gene            complement(17767..18108)
                     /locus_tag="VFI55_14385"
     CDS             complement(17767..18108)
                     /locus_tag="VFI55_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HET7075256.1"
                     /translation="MCSLNELPPGSMKLVDVGKFGVGVFNVRGALYAIANYCSHEGAP
                     LCMGLIGGTTESAPESPGGIRRVRDGQIIRCPWHNWEFDVTTGQNVADPRRRVRTYQI
                     DVSDGEVYLTA"
     gene            18293..19528
                     /locus_tag="VFI55_14390"
     CDS             18293..19528
                     /locus_tag="VFI55_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7075257.1"
                     /translation="MTLSTDHVDPEGQPVTLDLTGETSPYPFFEYMRRTDPVWHGALS
                     DSSQMPEELRPNDEWALFGYDALFQAFRDDRGFGSHKYDETIGLVMGHTILAMGGPEH
                     HDHRNLVAKAFRATALQRWEPSVIGPVCDRLVDEIKNDGQADLVKAVTFEFPTRIISS
                     LLGLPPEDLDLFRRLSLDLISIQTDIMAGLNAAAELHGYFLEQVGQRRRKPTDDIIGD
                     LVAAEIDGEKLTDEAIIAFLRLLLPAGLETTYRSSGNLLYLLLTHPEQLAMVDRDRSL
                     IPAAIEEGLRFETPLTMVMRTTTHEVEIGGKTIPPDAQIDMCMGSANRDERRWTDPET
                     FDIRRRRQAHIAFAGGIHMCLGMHLARLETRVMLNSLFDRVTDLAFAPDDGTGEESKI
                     VGLTFRSPNKLPVTFAPVA"
     gene            19525..20706
                     /locus_tag="VFI55_14395"
     CDS             19525..20706
                     /locus_tag="VFI55_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HET7075258.1"
                     /translation="MTGRRKSRGAILHDVGGPWSVEEFELDPPRAGEVLVEMAAAGLC
                     HSDDHILKGDLSAPNDVMRSLGLPTMFPTIGGHEGSGIVREVGSGVTDFAPGDHVVMS
                     FVAVCGQCRWCATGMEYICDVGAFTLTPGMPTDGTFRHHTADGRPLGHLAKVGAFAKH
                     TVVATNSLVKIEPDLPLGPSALLSCAIPTGYGSVANRSGMRAGDTIVVVGVGGIGTGA
                     VQGARIGGAAQIVAVDPVDFKQKSALQFGATHTAATIAETLDLVRDLTSGVMADAVVV
                     SPSLITADDVRDALKLTRKGGTCVLTGMTSQLTRAVNIDLQDFILMNKTLAGTIFGSC
                     NPKADIFRLARLYQTGQLQLDEMITRRYRLDEINDAYEDLRNGKIIRGIIDFGIEPGL
                     T"
     gene            complement(20792..22123)
                     /locus_tag="VFI55_14400"
     CDS             complement(20792..22123)
                     /locus_tag="VFI55_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7075259.1"
                     /translation="MDRLLTGDELPQREGVTSRGAAWPVLEYRRLAVEREALRRVANL
                     VATGAEPSEVFDAVTREMCRCVSASNAGLWRYDAGGEITKLAGAEVPTALAKWAVGTR
                     TRVDGNTLAAIVYSTGRPARLDGYDDATGAVAALVRETGVRSAVGVPVIVDGRVWGLA
                     AVGSVHPGPMPADTEARIGVFAELIAAAIVAGYRDEQKRQLLGGESQRPLLVDSLLYG
                     RILDQWGVWEIANQLRLPSQGSFVVVAAEVSVVGTVPLSEIESKLRSLDVYSVWRVLP
                     DLQVGIVHVKSEQHLDKILALLSRTTTDRVGVSARFDDLRDTPRAFHIAKVTLRNGAR
                     DANRVAVFDGSILATAAVSAPEAMVKSVATVLDGFNDLGEEERELLFETFRVWQDSDA
                     SVRDTAEVLICHPNTVRYRLRRIEQRTGRSLSRPRDIAELCLAFEVQRRLM"
     gene            complement(22327..>24292)
                     /locus_tag="VFI55_14405"
     CDS             complement(22327..>24292)
                     /locus_tag="VFI55_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET7075260.1"
                     /translation="RLLLLVAAADSSADPGLVWRAAARLGIGPEAAAPANEAGVVQFS
                     SRVSFRHPLVRSVVYRSASLHERQMVHGALAEVTDPGHDPDRRAWHRAHAAPAPDDEV
                     AAELERFADRARARGGIAAAAAFLEQAAMLTLDPTRRSDRALAAATAKVEAGALDAAQ
                     ELLATAGCGPLSDLQQARIDMIGAELAFVASRGSDAPSLLLKAARRLESIDAGLSRAT
                     YLQALSAGMFAGRLALGGGVYEVARAAAAAPPPPGPTRALDLFLDGLVAHFNDGYRAG
                     LPILRRALQGFGTGMSPEEELRWHWVAGIVARHVWDDGAWRRLADRHVKLARDIGALS
                     ELPLALSSCAFKLAFAGELTAAGALMQELRAATEATGIKLAPYAALGVAAMRGCEAET
                     AALVGATIDEVRLRGEGIGITVAQWATAVLNNGLGNYHIAMTAAQHAIEYPGELVAPA
                     WASAELVEAAARTGNRDIGADCLRLLTDVTSMAGTDWALGIEARSRALLSEGEPAERL
                     YRESIERLGRTEVRAELARAHLLYGEWLRRQRRRVDARAQLRTAHDMLDGIGMEAFAE
                     RARRELQATGETVRKRTVSMPDQKLTAQEAQIARLASDGLSNPEIGARLFISARTVQY
                     HLHKVFTKLGIESRGQLARVLRERPAELLR"
BASE COUNT         4186 a         8206 c         7919 g         3981 t
ORIGIN      
        1 ggactcgact cgctggccac attgcgtcca agcgacgata tctgcaccga gctgggctcg
       61 ctgctcgccg acgccgaggc gctgcgggcc accggctacc gtgcgctggg caacgaactg
      121 gccggacgac ccagcccgga agccgacatc ctcaagctgc tcggatcggt taccttgcaa
      181 cgggtttggg aactcaccgc cgtcgcccgc ggaccacagt cggccagcga tgcggatctg
      241 ctcttcgaac gccaggacgc gatggccgcg accatctacg gcggaacatc ggaggtccag
      301 cgcaacatca tcgccgagcg cctgctcggg ctgccgaagg gatgacgagg atggactacg
      361 agctcggcga ggacgccgga cagctgcgaa accatttgcg cgagctgatc tcccaccaca
      421 ttcccgccga ctttctgggc gcctgcaccg acgatccgcg cgacctggcc accaccgaga
      481 cgttctgcaa gctgctggcc gccgaggggc tgctggcgct ggcgtggccg aaagagcatg
      541 gcggcggcgg tggttcgatc tggcagcaga cggtgctgcg cgaggaaatg tgggcccaac
      601 acgagccccg cggcccccag tacatgggaa tcaactgggt cggtccggct ctgatgcact
      661 acgggacggc cgaacagaag gccaggcacc tgtcggccat cgcggccggg gacgtgatct
      721 ggtgccaagg gttttccgag cccgaggccg gaaccgatct cgcctcgctg cgcacccgcg
      781 ccctacccga cggagacggc tggcgaatca ccggacagaa ggtgtggacg tcgtacgccc
      841 ggatggcgtc ctggtgcgtg ctggcggcgt gcaccgaccc cgacgcgcca aagcacaagc
      901 ggctcacgct ttttctgctg ccgatggacc ggccgggttt caccgtgcga ccgatcccgt
      961 cgatgctggg accccatcac ctcaacgaga tgttcctgga cggcgtgcag gccttccgag
     1021 gtgacgcgct cggcgagccc ggcgacggct ggcgcgtgat gcgcgaggcg ttggctttcg
     1081 aacgggtcgg catcgcgcgc tacgcccggt gcgaatcact gctcgaccgg atgatcaccc
     1141 aactcggcga cgactgggac cggcttcccg aaaccatccg cgcccgctgg gtgcgtgcgc
     1201 tcgtggacct acgggtggcc agactgctgg cctatcgggc gatttcgctg caggatgatc
     1261 cgtcggcggg cgcggcggcc agcgccgccc gcatcgcgac caccacctgc gatcaacagg
     1321 tcgccgagtt gctgttcgac gtgttgggtc cggccgcgct ggacagcggt tcctcggcgc
     1381 cgctgcacgg ggcgatcgaa gaccactggc gctacgcgca ggcggccacc gtcgcatcgg
     1441 gcactatcga agtgcagcga atgttggtgg cacgggacgt cctgggagga caccggtgaa
     1501 aaccgtactc ccacaagata tcagcgactt cgcggccgtc gccgccaagc gactcgcccg
     1561 gttgggcgga ccaccggcgg cgctgcgcgc cgagaccgac gacggcgtcc gggccgcggc
     1621 gcgcatcgcc ctgagcgacg tcggagcatt cgagcttgac gtccggtcgg cgcccgacga
     1681 cctgctggcc gccgccgtgc tctgccaggc cgccggcgcg accgctctgc cgtatcccct
     1741 cgtcgaagaa ctgctggcga tcgagggcgc ccgactggcc ctggtcaatc ccgaggcacc
     1801 gcgcatcgac cacggcgacc tgcccggtgc gtggaccgcc gccgacctgg acggcattcg
     1861 gtaccacccg caaccggcgt cccggacgag cgccaaattg gggccgtttc tggtgccggc
     1921 gactctgagc gcgcccgccg gatccgtccc ggccgccgac gtcaaccttc atctgatcct
     1981 gggatcgtgg tggattctgg gaacgatgca gcagtcgctg cacatcgtca ccgagcatgt
     2041 gcgcgcgcgc attcagttcg gcaagccgct ggcggacttc caggccgtca ggttcgccgt
     2101 cgcggatgcg gccgtcgcgg tacgcggact ccacgagctc gcgaagtaca ccgtctgccg
     2161 accgaaatcg ctaccggctc gggcgcattc ggccgacgcg ctggtgctcc ggtacaaggc
     2221 cgccgacacc gcgcggcagg tgctgcgcac ctcccatcag ttgctgggcg ccctgggttt
     2281 ctgcgacgag tccgacatca gcgtgctcga ccggcacacc cagccgctca tccggttgcc
     2341 gctgagcgcc gaggcgctgg cgttgcgcct gatacccgac gtccgcggcg gccagctgga
     2401 gacgctgttc agcgggccgg tatcggcatg agccgcgccg gatccgtcga tgccggtgtg
     2461 acttccaacc cgctcgaaga agggatcccg ttcgggacca aactcgccga gctggccgaa
     2521 cagcgtcgga acgatgccgc cgtgaccatc gtcgcgctcg acggcaccgc ccagtcgctg
     2581 acctttgccg aactcgacgc ccgcgccaac caatgggggc gggcactggc cgcccaccgt
     2641 gccgagacgg gttcccttgt cgcgcttgcg atcccgaact cccagcacct ggtgctggcc
     2701 acgctgggat gctggaagat cggtgcggtt cccattccga tgcattggga tctgcccgac
     2761 tgggagcgga accgggtgcg tgacgtgatc gaccccgccg tcgtcgtcga cgaaaacagc
     2821 cggtgggagc tggatgcgcg cgcggcgcgc gaatccgaaa gtcccctgcc cacagccgtt
     2881 tccccgaccg ccaacggaat atgcagcagc ggctcgaccg gagtgcccaa agtaatcctc
     2941 aacttggcgc cgtcgctgtg gacagatcag cagggcgagc cgtttttgtc gaactggata
     3001 ccggtggcca agccgcagac catcatggtg cccgccccga tgtatcacac caacggcttc
     3061 gccacgttcc tcatgctgct gagcggcgac cacctggtgg tactcgaaaa gttcaacgcg
     3121 gcactggccc tggacgtcat cgaacgcttt cggatcacca acttcacggc cacgcccacg
     3181 atgctgtcgc gcatcgcggc ccggcccgat gtccggcagc gaaacctgtc cagcgtggtc
     3241 ttcatcctgc agggcgcggc cgtgatgccg ccgtcgctgc tgcacacctg gttcgagctg
     3301 ctgagccccg agcagatcgt cacggcctac ggcatgaccg aaaatctcgg cctcaccgcg
     3361 ttgcgcggcg acgaatggct tgcccatccc ggcagcgtgg gccgcggttt ccgggacacc
     3421 gagatccgga tcctggacgc cgagaagaag ccggtcggcc cgggcgaaca cggcgacgtc
     3481 tacctgcgcg cgccgatgag cgcgggatac cgctatctgg gcggggcccc gccgctgccg
     3541 tcgaccgacg acgggtttcg ttccgccggc gacgtcggct gcctggacga ggacggctac
     3601 ctgcacatcg tggaccgccg cgtcgacatg atcgtcaccg gcggcgccaa tgtctttccg
     3661 gccgaggtcg aatccgccct cgcgggccac ccgggcatcg ccgacgtggt ggtcatcggg
     3721 ctctccgacc cgcgatgggg acgccgggtg cacgccgtgg ttcagctcac cgacgcagat
     3781 ggccgcgccc cgctgaccga gcagcaggtg atcgggtacg cgaagagtcg cctggccggg
     3841 tacaaggtgc ccaagaccgt cgagttcgtc gccgcgattc cgcgcacggc ggccaccaag
     3901 gtcaatcggt cggcgatgat cgcggcccgg ggcggctagc cgtagcgccg cgcaacgcgg
     3961 accctgtcac aacgtatccc ggcggccgcc cggcgcgtcg cagtttcgaa ttcggtccgg
     4021 gcacatctga atcagacccg aattgcgggt ctgaccagca aagctgtgtg gtgatacatg
     4081 tcccactttg gcggccttcc cgacatctca ctgcagccag actgtaggtt tggacagcaa
     4141 tgaacgacaa cgacgtgccc gctacgcgca acgggctcga caccgacttg accgccgaga
     4201 acggcgcaga gcttcgccag gtctgccaaa cgtcggactt cgcccgttgc atttgctgcc
     4261 tggccgccgc accgggttgc ggcgtgacct acgacatcgc atttcacgtc cgccgcctgg
     4321 tgctttccgc atttgtgggg ccgtgttgtg cggacaagga gcgccgccac tggcgcgacg
     4381 acacagcggg caacaacgtc tgggctcttc gccggacttc gcggagcgcc gacatcgaag
     4441 attgcgtccg caacgctggc ccccacacgg cccacacagc acattgcgca ggcggacacc
     4501 cacattcacc cgagaacacc gggccacgca aaccgcgggg acgccgaccg gttacacgtg
     4561 tccctcggga ttgccgccat gggtacgcgc gtaacagcta ccgcctccag gcggcagcgc
     4621 gccgccggcg aggggcgccg tcgaaggtgg ccgcgtgacc tcgcctccga tgcacttcaa
     4681 ggagagtccg tttttcgcgg cagaccgcag cgcctattgg cgcgagctgc gcgggctggg
     4741 cccggtggtc aaaatcgacg taggcaatcc aatgctgcgc ggctactacc tcaccgcgcg
     4801 cgacgatgtg cgcgcggctt tgcttgatcc ggacacgttt gcgtcgccgc cgaaaacctt
     4861 caacgtgttc ttgttcggcg aactgcttcc tcaggtgccg ctctcgtgcg ggacgcgatc
     4921 ggagcatgcc cgtttcggcc gcatcttgca tcctctgttc agccccaagg ggttggcgcc
     4981 cttcgcgccg gcgctgcgcg cccgggccac gatggtggtc gacgcggtcg ccgccaaggg
     5041 gcagtgtgat gcggtcgacc tcgccgacac ctacgcctgt caagcgctgg tcaccgtctg
     5101 cgggctgccc gccgacgacc ccagggcggt tccgctgacc cgggccgcgg tcgtcggcga
     5161 cgccgacggg acagcccacc ttgcgctcat caattggctg ctgtccggcc tgaacgaggg
     5221 gacgagaaat cccgagcggc cgcccggcgt gctctggccg ctcctcgagg gactcgacgg
     5281 agataaggat tttccgctca gcccggtcga agtggtagcg gtgatcctgt tgttgttctc
     5341 tgtcgcgggc attgaaatgg ccgcctcggc aatttctttc atgctgctac atctcgggcg
     5401 cgatccgcga ttacaggccc agctgcgcga ggacccggca cagattccgg cgttcatcga
     5461 ggaagtcttg cggttggaaa cgccggggcc ggccatcccg cgcatcacca cgagggaagt
     5521 ggagatcggt ggcgtcacga tcccggcggg taccgcggtg tggctggcct tggaggccgc
     5581 cggccgagag aacggcggcg acgagatagc taccgcgagc aatggagaga ttcagcggca
     5641 gcgccactgg gcgttcggcg tcggcatgca cagatgcttg ggcatgcacc tggcccgtct
     5701 tgagattgcc gcgttcgtgg gcgaatggtt gagccgtatc ccgcaattcg agctggagcc
     5761 gggcttcacg ccggccattg tgcacaaacc aaccggcgtg acccacctgg gcagcttgaa
     5821 gctgcggtgg gaggcgcgct gagcgcgccg ccgacgcggg cgatctagct tcgcggatct
     5881 gtcacaccac gcggtaacct cattctcgaa gcgcaacccg tgcgcaacgg aaggggacga
     5941 agcgctgatg gccgaccccg gcgaggaagc tgaagtgacg cgcgccgacc gctttatcaa
     6001 gacggccgtt gcgatactcg gcgaaacggg acgcaccgac ttcaccgttc aggaggtcgt
     6061 cgcgcgttcc aagacctcgt tgcgggcgtt ctaccagcat ttcagtagca aggacgagct
     6121 gctgctcgcg ctgttcgatc ggaccattgc gcaatccgcg caatcctggc gcgccgagac
     6181 ggcagggctg gacagcaccg ccgcgctgaa gctggtcatc gaccgcatca gccagcaacc
     6241 cgaatcaagc acccaggaca gcctcaatcg ggcgctgagt ctctacaacc agcatctggc
     6301 cgagacacgt ccgcgcgagt acgcccgcgt cctctccccc ttacaccacc tgattcgcga
     6361 catcgtgggg caaggcatca ccgagggcgt cttcaatccg ggcctcgacg tcggtgccgc
     6421 cgcagcgatc gccatgcaga cgatggtcgg tgcgcagcga ttgcactggc tcggtagcga
     6481 attgaacggc actccggtcg acgcgggcca gctgtacgac ttctgcagcc gcgccctggg
     6541 catccgggac accgacgacg aatcggccgc cccctcgctg gccgaactgt ttgcccaaat
     6601 cgggatgcgt cccggcaccc gtgacggcca gttcgcgatg accatgccgg tcagccccgc
     6661 ggtggtcaac acctccggcg cgctccaggg cggattgatc gccacgctcg tcgacgtggc
     6721 ggggggacag ttcgggctgg attatctgca gccgggcacc accatgacga ccgcagacct
     6781 cttcgtccgg tacctgcggc cgatccgccg gggcccggca ctcgcggtcc cgcgaatgct
     6841 gcggacgggc cggcgggcga tggtcatgca ggtcgacatc tacggcgacg gtgacgacga
     6901 actcgccgcg acggcgaccg tgaatttcgc catcatcaac ggcgacacgc cgaccgtggg
     6961 tctgggcgcg atcacctaag cgatcaaagg gccggtgcac cttgccacac agaagtggta
     7021 acgtcattac cgcctagaga gaaccccggc cttgcgcaag gagatcgcca tgccgtctcg
     7081 cgaactttcc ttccccgtgt tcgacgccga caaccacatg tacgaaccgc aagaagcact
     7141 gaccaagttc ctgccggaca agcgcaagca cgtcatcgac tatgtgcagg tccgaggtcg
     7201 caccaagatc gtggtgcgcg gccacatcag cgaatacatc cccaacccga cattcgagaa
     7261 ggtcgccaag ccgggcgccc aggaggacta cttccgccac ggcgcgcagg gcaagaccta
     7321 ccgggagatc ctgggcgagc cgatgaaggc catccccgcg ttccgcgaac cggggccacg
     7381 acttgaggtc atggacgagc tcggcatcga ctacgccctg atgttcccca ccctggccag
     7441 cctggtcgag gagcgcatga aggacgaccc cgagatgacc cacgacgtca tccacgcgct
     7501 caacgagtgg atgcacgaga cctggacgtt caactaccag gagcgaatct tcgccacccc
     7561 ggtgatcacg ttgccgatcg tggaccgcgc gctcgaagaa ctgcagtggt gcctggagcg
     7621 cggcgcccgc accgtgctgg tccgccctgc gccggtgccc ggctaccgcg gtagccgctc
     7681 gttcggcctc gaggagttcg acccgttctg gcaggcctgc atcaaagccg agatcccggt
     7741 ctcgatgcac gcctcggaca gtggatattc cgagttcgcg aacgtgtggg agcccggcga
     7801 cgagttcctg ccgttcaagc cgaccgcctt ccgcagcttc gcgatgggcc atcggccgat
     7861 catggacgcg atggccgcgc tggtgtgcca cggcgcgctg tcgcgcaacc cggaactgcg
     7921 gatcctgtcg atcgagaatg gcgccgactg ggtgccggac ctgttcaagg gcctcaaggg
     7981 cgtctacaag aagatgccgc aatcgttcac cgaagacccg gtcgaggcgt tcaagcgctg
     8041 cgtctacatc acgccgttct gggaggaccg gttcaccgaa atcgtcacga tggtgggcac
     8101 cgaccgcgtg ctgttcgggt ccgactggcc gcaccccgag ggcctgaagg acccgatctc
     8161 cttcgtcgac gagctcgccg acttcgaaga agcggatgtc gccaagatca tgggtggcaa
     8221 cctgatgaag ctgatgaagg tcgccaagcc agtctcggcc tgatcgccat tcgggtcctg
     8281 ccacggccgc gggtgggatc gccgattacc cccgcgcggg cggggcgatt ccggcgtttc
     8341 tcgtgaaagc cgttagcaaa ctgttgcgtg aggcagagca cagccgtaga taatctgagt
     8401 tacggcgtct ggggagtgcg acaacgcgtt tgacccggtt tccgactcgg tcctgagcag
     8461 gtccgcagca aggctccgta ggagggacgg ccagatggtg gccgacgagg actgggacaa
     8521 aacagtgggc gcggccgatg aagtgcgccg catcttcgaa accgttcccg cgatgctggt
     8581 tggtctggac gggcccgacc accggtttgt cgcggtcaac gccgcttacc gcgccttcaa
     8641 tccgacgggc aaccccgtgg gaaagcttgc gcgcgaggtc ttccccgagc tggagagcca
     8701 gcagatcttc ctgatgctcg accgggtgta cgagaccgcc gaaccacaat ccgggtcgga
     8761 gtggcgcctg caggctgact atgacggttc aggcgtccag gaacggttct tcgacttcgt
     8821 cgtcacaccg cgccgccggc aggacggatc ggtcgagggc gtgcagatca tgttcgacga
     8881 tgtcaccaac cgggttcagg cacggctggc cgccgaagcg cgcatgcagg aaatgtccga
     8941 gcgctaccgc agcgtccgcg attccgccac cgtcatgcaa caggccttgc tggccccgtc
     9001 cgtgcccgtc gttcccggcg cggacatcgc cgcggagtac ctcgtcgccg cggaggacac
     9061 cgccgccggg ggcgactggt ttgatgcgat cgccctcggg gatcgactgg tgctcatcgt
     9121 cggcgacgtc gtcggccatg gcgtcgaagc tgcggcggtg atgtcgcaac tgcgcaccgc
     9181 gctgcgaatg cagatttcat cgggacacac catcgtcgaa gcgctcgagg ccgtcgaccg
     9241 ttttcatgaa cacgtgcccg ggtcgaagtc ggcgaccgtg tgcgtcggtt cgctcgactt
     9301 cgccacgggc gaattccagt actgcacagc cggacacccg ccgccgctgc tggtcggcgc
     9361 cgacgcgaac tcccgctacg cggaaccctc cggcgccggt ccgctcggca gcggcaccgg
     9421 gtttccggtg cgcacagagg tgctcggcgt gggcgactcg gtgctgctgt attccgacgg
     9481 cctcatcgaa cgtcccggcc ggccgttggg cgcgagcacc gcggaattcg ccgacctggc
     9541 cgcgagtgtc gcgggcggcc gtggcggctt cgtcatcgag gccgcgcgac ggcccatcga
     9601 ccgaatctgt tcggagacac tggaattgct ggtccgatcg accggctaca acgacgacgt
     9661 cacactcctt ggggcgcaac ggcgcacacc gccgcgaccg ctgcacctga cgctgggcgc
     9721 cacgatcgac accgcacgca cggttcgcgc gcggctccgt gaatggctgt cggagatcgg
     9781 cgcggacgcc gacgacacct gcgacgttgt acacgcggta tcggagttcg tcgaaaacgc
     9841 ggtcgagcat gggtacgcaa ccaaggacgc acccggggtt tcaaacacga tcgtccttga
     9901 ggctgcgctg ggcggcgacg gcaacctgcg cgcgtccgtg atcgaccagg gatcctggaa
     9961 ggattaccgc gagggcgagc gcggtcgcgg acgcggactc gcgatggccg aggccctggt
    10021 gtccgaggca cacatcaccc gctctgccgg cgggacgacg gccagcctga cgcaccgcct
    10081 gtcgcgacag gcaaatttcg tcaccgacac aaccttcagc cgcgtgaccc agcagcggct
    10141 ggtcaacccc gaattcgtct cgatggtcag ctcggatgga ccggtcggcc ggatcgtcgt
    10201 cagcggcgac gtcgactcca ccaccgcgtc caccctggac cgtcagatcg cagtcgaaag
    10261 ccgttcaggt atagcgcctt tgacgatcga cctgagcggg gtcacccacc tcggctcggc
    10321 gggcgtcagc gcgctggtcg ccgcccgcga ccgggcccgc aaacagggcg ctgagtgtgt
    10381 gctcatcgca ccgccgggta gcccggcgca tcacgtctta tcgctcgtcc agatgcccgt
    10441 taccggcggc gacgtcgaga acgtcttcgt cgacgaatag cgcaggtccc gtcgcggtca
    10501 gcgcgtctgg ccgtgccgca cctggttgaa cgggacccca cgatcggcgg cgtattcccg
    10561 tggcaggccc agcacccgct caccgatcac gttgcgggcc atctcggtgg taccgccgcc
    10621 gatcgcgacg ttctgccggg acaggtaccg cagccccgtc tgcaggcctt cgccgatctc
    10681 ccctaccacg ccggcggctc ccgcgatcgc cagtgccgtg tccatatcca gggtcaccgt
    10741 ctcggagtgg aagagcctga taagcgttcc ggcggccggg ggcagtacgc catctcggac
    10801 atttcggtag acgtgatcga tcagctgttc gcccaccgcg cggtgcacca gtacccggcc
    10861 ggccatctcg tgcacgcgtt cgttgtcggc ctggcccgtc atctccgcaa gggcaacgta
    10921 gtcaacgggt ttcgccgtcc cgccctcact gccgctgccg ctggcgaatt ccgaaccctg
    10981 tccgaccgcc cggcgctcgt gatacagctg ccgcgaggcc accgcccagc cgccgttcac
    11041 cccgccgacg acggcgtcgt cgccaacgtc cacctcgtcc aggaactcct cgcagaactc
    11101 ggtggagccg ctcagcatgg tgatgcgacg caacgtgatt ccccgatgag cgatcggcac
    11161 caggaacatg gtcaggccct catgcttggg cgcatcccag ttagtgcgtg ccagacacag
    11221 gccgtagtcg gcggcgaacg cgctggtgct ccacgtcttg gcgccgttga tcacccagcg
    11281 gtcgccgcgg cgctcggccc gtgtgatgac gccggccaga tccgacccgc cgctgggctc
    11341 actcaacagc tgcaccagca cttcatcgcc gcgcagggcg gcggcgatgt gccgcttctt
    11401 ctggtcttca gtgccggtgt ctagcagcgt ggcgcagcag atggtgaacg tgggggtgtt
    11461 gagaatcaac ggcatctcgt agtcgagact ttcggagtcg aatgccttct ggtactcgta
    11521 atccagaccc aggccaccgt agtcggacgg gaagcaaata ccggcgaatc cgccgtcata
    11581 gagccgccgt tgcagctctc gcgcccgctt ccaggaccgt tcgttgtcgc gcggggcggg
    11641 cggtggcgac tcgtggtcga tggcaggcat gttgtccgcc aaccacactc gggcccggaa
    11701 ggcgaattcg gcgacggatt cggtgggcat catcgggcgg tcgtcatcga tcgggccgcc
    11761 ttctccaacg catacaccct caggttgtgc tcctcgggcg taccgaacat ggagcggtac
    11821 aacgtgaccc gcctcaggaa caggtgcagg tcgtgttccc aggtgactcc gatgccgccg
    11881 tgcatctgaa cgcacgcctg aacgatgcgg ccggccatct cgccgacata ggacttggcg
    11941 atgctggccg acaactccgc cgcgggcgag cgcgcggcaa cgtcggcgac cgcggcggcc
    12001 gtggtggcac ggcatgcttc cagccacagt ttcatatcgg cgaagccgtg tttgagggct
    12061 tggtacgacg ccagcggacg accgaacgtg tgccggtcca gcgcccactg gacggtgaag
    12121 tcgaacaccg tttgcaggat gccgaccacc tcggcgcact gcagcacctg ggcaatctgg
    12181 ccctgccgtt cgatcaacgt ggcggtcccg ccggccccgc cgaccaccgc ggacggctcc
    12241 acctgtacgc cgtcgaattg cacccgcgcg tattgcttga ccaagtccac cgacctctga
    12301 ggctcgatcc ggacgcccgg cgcgtccgtg ggaaccaaga actgacgaac ctcctcggca
    12361 gcttggccac atcgcgccac gaccagcagc accgcgctct gggcgcccgc ctcgacgcgg
    12421 tccttggtgc cgtcgatgcg aaacccggtc tcggtccggg tggccgtcac cgacggatcc
    12481 agcggcgccc atccccgccc cggctcggaa accgcccacg acgccacggt gtcaccggaa
    12541 accagggact cgatggtcgc gacgtgcgat tgcgggtcag cgcagtcgac gagggcggca
    12601 agcacgacgc tgaccggata cagcggcccg ggtgccaccg ttttgccgag ctgttcggca
    12661 accatggcca gatcctcgac accgctgccc gagacgctgc cgccgcccag ctcctcggga
    12721 accagcagag ccgtccagcc gagctcggcg gcgcgccgcc accatgccgg atcgaaggac
    12781 acgccctcgg catgcagttc ccggacacgg cgcagcgggg cttctttgtg cagaaaggct
    12841 tgggtcgtgg aagcgaatag tatcttttcg ggagagtcga cagcggtcat gatgcaggca
    12901 ggtccgttac cgttctatcg ccgctgtgaa ggacgcaggg gagaaagttg gggtggttga
    12961 tcatccgcct gatcggttct cgtcggagtt cgctggggac tctgatgtta gcaacgagca
    13021 atacggtaag agataccgga gttctgtcga taatgatgtt gacgagcggt aatcgcgatg
    13081 gctgagacga acgaacccgc agcgggttcg gcccctcccg agtccggcgc caaggcgttg
    13141 atcgaggaag ctgaggcaga agccgccgcg gccgaagcac tggccgccgc ggccaaggcg
    13201 cgcgcccgcg ccgcccggct gcggcgccaa gccctgggcg tgcccgaccc cgccgaagat
    13261 tacgacgccg accgggacca cgacgaggac tacgacgaag actacgacga ggacgaagac
    13321 tacgaggccg ccgacgaaca ttaccccgag gactacgacg aagaatatgc cgaggactac
    13381 gacgagtcgg gcgccgaggg tgacgacgag accgtaaccg agcaagccag gtggcgacgc
    13441 cggatgccga cgatcgccgc ggtggcggca atcgtcatca tctgcgcttt tgtcggcctc
    13501 agcgtgtaca tggtgttcca gcatcgtgat gccaccaagc accaacaacg cgaaacggcg
    13561 ttcgtcggcg gggccaagca gggtgtcctc aacatgatct ccctcgactt caacaaggcc
    13621 aaggaagacg tgcaacgcgt gattgacagc tccaccggcc aattcaaaga cgacttccag
    13681 aagcgggcca acgatttaat cacggtggtc acgcagtcga aagcggtcac cgaaggaacc
    13741 gtgaacgcgg ccgccatcga gtccatgaat ggcaactcgg gggtggtact ggtctcggcg
    13801 acgtcgcagg tcaccaattc cccacccgga aaggacgagc cgccaaaagt gtggcgcctc
    13861 agggtgaccg tgaccgacgt cggcggacag tacaagatgt cgaaagttga gtacgtaccg
    13921 tgaccgacga aatgcgcgac acaaccgccg ttgcggccga cgacgattcg gacgcttcac
    13981 aagccacaac cgaagccgac ggctcgacag gggaagcccc gggcggcgtg gcggaggccg
    14041 agtcccccga gcctctcaag cgcccgcgat tcagcctgcc gccccggctg cgcaaattcg
    14101 ctccggtgat tctggcggtt ctcctgctga tctccggcgg tgtggcgacg tggctgtact
    14161 tcaagcaata ccggcccgat caacagaccg acgcgagcgt cgcgcacgcg gtggtgagtg
    14221 ccgcgtctga tggaacggtg gcgttgctgt cctactcatc cgactcgctc gacaaggact
    14281 tcgccaccgc caggtcgcat ctcgccgggg acttcctgtc ctattacgag caattcacgc
    14341 agcagagcgt ggctccggtg gccaaacaaa agtcgatgaa gacgacggca cacgtatcgg
    14401 gcgccgcggt gtccgagctg catcccgatt cggccgtcgt actggtgttt gtcgatcaga
    14461 gcaccacaac caaggactcc ccccagccga cattggcgct cagcagcgtg ctcgtgcaca
    14521 tgacccggat caacgggacc tggctgatca cgaaattcaa cccggtttag accgctacat
    14581 cgcgaacacg cggcgcagcg tctgcgcgtg cagggcccgg ttttcttggg agacaaccca
    14641 atccggaacc aacgaatcga agccgttgaa cgtcgctgcg ataacgtgca attcggtgtg
    14701 cacgtaggca tgcagtagcc ggttcgcgta gtcgatggct tcgtcgcggc acgggtcgat
    14761 ctcggagcag ctgatgaacg tcggcggcag cccctcgagg ttcgcccggt gcgcggggac
    14821 gtgctgaccg cgggccgtcg agtggccgag gtagtggttc catgcgcggc tcacggcggg
    14881 accgttaagg cccggcgtgc gctggaactc gcgacgcgac ggcgtcgcgt cggaatcgag
    14941 catcggctgg tgcaggatct gcaccaagat ttgcggaccc tcctcgtcga acatgcgctg
    15001 gctgaggccc gcgaccagcg ccgcacccgc gtcgcggccc atcaccgcga tccggtcggc
    15061 gtccgcgtcc aggtcggcgc agttctcgac ggcgtatcga agggccgctg ccacgtcggc
    15121 cagtgccgcc ggacacgggt tgtcgggtgc cagccggtag tcgaccgaca ccagtagaca
    15181 actgccggct cgcgccagct ccacgcactc cgcgtgatcg gtgtcgaggt tgcccgtcac
    15241 gaatccaccg ccgtgcgcgt agaccaccat gggtgccggg gactcggtgc ggccgcgata
    15301 cagccggatg tccagctgcc ggccgtccgg accggcgatg gatcgcgatt cgacgctgac
    15361 gccgtcggtg tcggccgccg cggcccgccg ggcggccaga aggttggcgt gttcgcgttc
    15421 ggcgggcagc gtctcggagc ggaattccac gacaccgagc tcggtcgcgg cctcccgcag
    15481 caccgggtcg atgcggttaa agccttgtgg ccgcgtgaga ttcgtttgtg tcatgcctga
    15541 ggcccgcgtc aacgttgccc gggcagggcc ttgacggttg ccggcaggcc atacagcgcg
    15601 gtcgcgttgc cgcgcatgat ccgttcgcgc tggtcggcgg ggaaccggtt gatcgcgtac
    15661 gtcggcgagt cgtaactcca gtgcgggtaa tccgttgaga acatgaggtt ctcgccgagg
    15721 tccatcatct ccaggtactc ccggaatccc atggtgtcgg tgtcttccag cggttgggtg
    15781 gtgaaccgga cgtgttcgcg cacatactcc gacgggcggc ggcggacgtg cggtaggtcg
    15841 ccgcggcggg cttcccagat ccggtccatc cgcgacatca ctggcatcgc ccaggtaaag
    15901 cctccttcga cgaacaccac acgcaggtcg gggtggcgat cgaacgcgcc gtcaaacacc
    15961 aggctcatca ggtgcgagac gtacagcagc ggccaggacg cgaagaagtc atgccagtgc
    16021 gcggggttgc cgaccggata cagcgggatc aactcgaacg gcgtctgacc catcagatgt
    16081 gtggccaccg gcaagcggtt gcgcgcggct gcctcgtaga gcgggtcgaa atgcgggttc
    16141 ccgaagggga ttccacgagt ctgcggtgtc atcagcacct gggccatgta cggatgtccc
    16201 gcccaccgtt gcacttcgcg cgccgcttgc tccggcgcct gcgcgctgac gctgaccgac
    16261 ccgcgccagc ggccgtgcgc gttgtgcctg tccagccaga cgtcggcgag ccagtcgtta
    16321 tacgtcgact tcagcacatg ctcggcctgt ggcagctgcg cgtcgcacat cggctccagc
    16381 atcgcgatgc tgacaccggc atccagcagc agctgcttgg cggcgaaatc cgggtcgctg
    16441 ccggcgaatc cgccgttggg cggcgcggca tcaaggcgca gggacatcgt tttgtacgag
    16501 tcgggggtgt cgtagaaatg cggcaccgga gtgaccgcat ttccggtcgg ccagatcttg
    16561 tccacccatt cggcgggcgc ataggacttc agcacgtcga ccgagacggg caatgggtgc
    16621 acatcggtgt cgacgatggt gaccgctatg tcgccttctt ggcccgtcgc cacgtcgatc
    16681 cgttcgatcg tggtcatcac acacgctcct tcagcccgta gagctcactg gcgtttcgcc
    16741 agaacacgtt gtcccgcttt gcctcgtcga gcccccgcag ctccgaggcc gccacctccg
    16801 gcgccgacgt ggaccaatgg ggataactcg atccgtacat caggaggtcc gacttggcgg
    16861 tgaagtccag ccagcgccgc atgtcgccgg cctgcatcgg cccgtcgaat gccgaccaac
    16921 aaaaccggac atggccgggc agatactcgc tgggatagcg gtcgacccac ggggtctggt
    16981 cgcgcatcga cagccagaag gtgtccaggc gccacatcag cggggtgagg atgtcgtaac
    17041 cgccgtcggc gaatacgaac ttgagatccg ggtgccggcc gaagacacct tcgacgatga
    17101 gcgtggccag gtgcacgaag tagttcagcg gcatgaatgc cgcgtatccc gggtaggtgt
    17161 gcgcgtgccc ggcgaaggtg ggtggatggt cgacaccgtt gccgccgttg atgtgtaccg
    17221 cgacgggcag cccgtgcgcg gcggcggcct cccagatggg ctcgaacacc ggtttgccgt
    17281 acggctcgcg tgattgcatc ggaacgccga cctgcaccag cttcgggtga gcagcgaggc
    17341 gctcgatctc ggcgaccgcg cccttggggt cttcgggatt gacgcgaatg gtgccgcgga
    17401 atcgattggt ggcgtcgggc tccagccaac ggtcgagcaa ccagtcgttg acggcggcgc
    17461 aaatttgggt gttgagcaga taatcggcga tatttccacg ggtcagcggg ttcaaaatgg
    17521 catatgccac ccgcaggtgc gagccggtgt cgtcgaatag ctgacggctg acggtctcgg
    17581 gatccgatcc cgggtagtgc tcaccgtaga ggtcttgccg gtagtcgccg ccgggcgcct
    17641 gataccactg ctgctcgacg tcgggaatgg atcgcaactg gtgggccggc gggagatagc
    17701 gccgaatttc ggcgttgtag cggaaatgcg gttggacgca ggtgtcgata atcggggtga
    17761 tcgggctcat gcggtcagat acacctcccc atcgctgaca tcgatttggt aggtgcgcac
    17821 ccggcgccgc ggatcggcta cgttttgacc cgtggtgacg tcgaattccc aattgtgcca
    17881 gggacatcga atgatctgtc catcacggac ccggcgtatc ccgccaggcg attccggagc
    17941 cgattccgta gtgccaccga tcaaccccat gcacagcgga gcgccctcgt gcgagcagta
    18001 attcgcgatc gcatacaggg caccacgcac gttgaacacg cccaccccga acttccccac
    18061 gtcgaccagt ttcatgctgc cgggtggcag ctcgttgagg gagcacaccc atcgccgctc
    18121 gagcaccggg gaatcaagcc tcctcagatc gtttcgggga gcatccgcac ctaatactgt
    18181 gcgctttacg gtaaccactt accagccgct tggcttcgtt gctatttata ttatgatagc
    18241 gatattccac cacaaacaaa cccggcttag cggccgctca agaggagcgc tgatgacgct
    18301 cagcaccgac cacgtcgatc ccgagggcca gcccgtcaca ctcgacctca ccggtgaaac
    18361 cagcccgtat cccttcttcg aatacatgcg gcgcaccgat ccggtctggc acggcgccct
    18421 gtcggactcc tcgcagatgc cggaggaact gcggcccaac gacgagtggg ccttgttcgg
    18481 ttatgacgcg ctgttccagg cgttccgcga cgaccgcggc ttcggctcgc acaaatacga
    18541 cgagaccatc ggtttggtga tgggccacac catcctggcg atgggcggcc cggaacacca
    18601 cgatcatcgc aacctggtgg ccaaggcgtt tcgcgccacc gcgctgcagc gctgggagcc
    18661 gtcggtcatc gggccggtat gcgatcggct cgtcgacgag atcaagaacg acggtcaggc
    18721 cgatctggtg aaggcggtga cgttcgagtt tccgacccgg atcatctcct cgctgctcgg
    18781 tcttcccccc gaggacctcg accttttccg gcgcttgtcc ctcgacctca tctcgatcca
    18841 gacagacatc atggcggggc tgaacgccgc cgcagagttg cacggttact tcctcgaaca
    18901 agtcgggcaa cggcggcgca agccgaccga tgacatcatc ggcgacctgg tcgccgcgga
    18961 gatcgacggc gagaagctca ccgacgaggc catcatcgcc ttcttgcgcc tgctgctgcc
    19021 ggccggactg gagaccacct atcgctcttc gggcaacctg ctgtacctgc tgctgaccca
    19081 ccccgagcag ttggcgatgg tggaccggga ccggtcgctg atacccgcgg cgatcgaaga
    19141 gggcctgcgg ttcgaaaccc cactgaccat ggtcatgcgg accaccactc acgaagtcga
    19201 aataggcggc aagacaatcc cgcccgatgc gcagatcgac atgtgcatgg gttcggcgaa
    19261 ccgcgacgag aggcgctgga ccgaccccga gaccttcgac attcgccggc ggcggcaagc
    19321 ccacatcgcc ttcgccggcg gcatccacat gtgcctcggc atgcacctgg cccgactgga
    19381 aacccgagtc atgttgaaca gcttgttcga ccgcgtcacc gacctggctt tcgcccccga
    19441 cgacggtacc ggcgaggagt cgaagatcgt ggggctcacg ttccggtcac ccaacaagct
    19501 tccggtcacg ttcgccccgg tcgcatgacg ggtcgccgga aaagtcgggg agcgatcctg
    19561 cacgacgtcg gcgggccttg gtctgtcgag gaattcgagc tggatccgcc ccgcgccggc
    19621 gaagtcctgg tcgagatggc cgccgccggg ttgtgccact ccgacgacca catactcaag
    19681 ggcgatttgt cggcgcccaa cgacgtgatg cgatccctcg ggctgccgac gatgttcccg
    19741 acgatcggcg gtcacgaggg ttccggtatc gttcgcgaag tcggttccgg cgtaaccgat
    19801 ttcgcgccgg gcgaccacgt ggtgatgtcg ttcgtcgccg tgtgcggaca gtgccgctgg
    19861 tgtgccaccg ggatggagta catctgcgac gtcggcgcct tcaccctgac cccggggatg
    19921 cctaccgacg gcactttccg tcatcacacc gccgacggcc ggccgctagg ccatttggcg
    19981 aaggtgggcg cattcgccaa acacactgtg gtggcgacga attcgcttgt gaagatcgag
    20041 ccggacttac cgttggggcc aagcgcgctg ctatcgtgcg ccatcccgac cggctacggt
    20101 tcggtcgcga atcgctccgg tatgcgcgcg ggcgacacca tagtcgtggt cggtgtcggt
    20161 ggcatcggta cgggcgcggt ccagggcgcc cggatcggcg gcgccgcgca gatcgtcgcg
    20221 gtcgatccgg tggacttcaa gcagaaatcc gccctgcagt tcggcgcgac ccacaccgca
    20281 gcgacgatcg ccgagacgct ggatctggtg cgggatctga cgtccggggt gatggccgac
    20341 gccgtggtgg tctcgccgtc cttgatcacc gccgacgacg tccgcgatgc gctgaagctc
    20401 acgcgcaagg gcggcacctg tgtgctcacc ggcatgacct cgcaactgac tcgggcggtc
    20461 aacatcgacc tgcaggactt catcctgatg aacaagacgc tggcgggcac cattttcggc
    20521 tcctgcaacc caaaggcgga catcttccgg ctggccaggc tgtatcagac cggacagctc
    20581 cagctcgatg aaatgatcac cagacgttat cgtctcgacg agatcaacga tgcctacgag
    20641 gatctccgga acggcaaaat catcaggggc atcatcgatt tcgggatcga acccgggctc
    20701 acctgagcct acttcttgca ggccatcacc cgggcaccgt cgagcggtcg ttcgtgctcg
    20761 cggcaaccac gcgcgctggc ggctcccggg gctacatcag ccgccgctgc acctcaaatg
    20821 ccaggcacag ctcggcgatg tcccgtgggc gagaaagcga ccggcccgtg cgttgctcga
    20881 ttcgccgaag ccggtagcgc acggtgttgg ggtggcagat cagcacttcg gcggtgtcgc
    20941 gcaccgaggc atcactgtct tgccaaaccc gaaatgtctc gaagagaagc tcgcgctcct
    21001 cctcgccgag gtcgttgaag ccgtccagca ccgtcgcgac cgatttgacc atcgcctcgg
    21061 gcgcgctgac ggcggcggtc gccaagatgg aaccgtcgaa taccgcgacc cgattggcat
    21121 cgcgtgcgcc gtttcgcagc gtcaccttgg cgatgtggaa tgcccgcggt gtgtccctca
    21181 gatcatcgaa gcgcgcgctg accccgaccc ggtcggtcgt cgtgcgactc aacagggcga
    21241 gaatcttgtc gagatgctgc tcggacttga catggacgat tcccacctgc aaatcgggca
    21301 aaacgcgcca caccgaatac acgtccaggc tgcgcagctt ggattcgatc tccgacagcg
    21361 gcaccgtgcc gaccacggac acctcggcgg cgacgaccac aaacgagccc tggctcggca
    21421 gccgaagctg gttggcgatt tcccacacac cccactggtc gagaattcgg ccgtaaagca
    21481 gtgagtcgac cagcaggggt cgctgcgatt cgccacccag cagctggcgc ttttgctcgt
    21541 cgcggtaacc ggccacgatc gcagccgcga tcagttcggc gaaaacgccg atgcgggcct
    21601 cggtgtccgc cggcatcggg cccgggtgca cggagccgac ggcggccagg ccccacacgc
    21661 gtccatcgac gatgaccgga acccctaccg cggagcgcac gcccgtctca cggacgagtg
    21721 cggccaccgc ccccgtggcg tcgtcatagc cgtccaatcg cgcgggccga ccggtgctgt
    21781 acacgatggc cgccagggtg ttgccgtcga cgcgtgttcg agtgcccacc gcccacttcg
    21841 ccaacgccgt cggtacttcg gctccggcca gcttggtgat ttcaccgccg gcgtcatagc
    21901 gccacaaccc ggcgttcgac gccgagacgc atcggcacat ttcgcgggtg accgcgtcga
    21961 acacctccga cggttcggcc ccggtggcca ccaggttggc cacccgccgc aaggcctctc
    22021 gctcgacggc aagccgtcgg tactcgagga ccggccacgc ggccccgcgg gatgtcacgc
    22081 cttcacgttg aggaagttcg tcgcccgtga ggagtcggtc catggctgcc tcctcgaatc
    22141 acacgtcgtg ctgttgaccg gctgggcggc cggtcgtgac cgcggcgctc ggatcaaaca
    22201 ccggtgtcga ccaacgtgtc gtcgacggcc atgctcggcc taatttggcg gcattcgcat
    22261 ctgccaagtg cacagtccgg gtctcacgtg gcgccttcca gacggtgccg gaagcgctgc
    22321 cgagggtcag cggagaagct ccgcgggtcg ttcccgcagg acgcgggcca gctggccgcg
    22381 cgactcgatg ccgagcttgg tgaagacctt gtgcaggtgg tactggacgg tgcgggcact
    22441 gatgaacaga cgggcgccga tctccgggtt ggacaacccg tcactggcca gccgggcgat
    22501 ctgggcctcc tgagcagtga gtttctggtc gggcatcgag acggtgcgtt tccggacggt
    22561 ctcgccggtg gcctgcagct cacgcctcgc ccgctcggcg aaagcttcca tcccaatccc
    22621 gtccagcatg tcgtgggccg tgcgaagctg cgcccgagcg tcgacgcggc ggcgctgccg
    22681 gcgcaaccat tcgccataca gcagatgggc gcgggcgagt tctgcccgga cctcggttcg
    22741 gccaagccgc tcgatcgact cgcggtacag ccgctcggcg ggttcgccct cgctcagcag
    22801 tgcacgcgac cgcgcctcaa tacccagtgc ccagtcggtg ccggccatgc tggtgacatc
    22861 ggtaagcaga cgcagacaat cggcaccgat gtccctgttc ccggtgcgcg cggccgcctc
    22921 gacgagttcg gccgaagccc atgcgggagc taccagttca cccgggtact cgatagcgtg
    22981 ttgagccgcg gtcatcgcta tgtggtagtt gcccaaaccg ttgttcagca cagcggtcgc
    23041 ccactgcgcg acggttattc cgatgccttc gcctcgcaac cgcacttcat cgatggtcgc
    23101 gccgaccaga gcggcggtct cagcctcgca accacgcatc gcggccacac cgagagcagc
    23161 gtagggggcc agcttgattc cggtcgcttc cgtggccgct cgcagctcct gcatcaaggc
    23221 gcccgcagcg gtcagttcac cggcgaacgc gagcttgaac gcacacgagc tgagggccag
    23281 cggaagctcg ctgagcgcgc cgatgtcacg tgcaagcttg acgtgtcgat cggcgaggcg
    23341 ccgccaggcg ccgtcatccc agacgtgtcg cgctactatc ccggccaccc aatgccagcg
    23401 caattcctcc tcgggcgaca tgccggtgcc gaagccctgg agcgcccggc gcagaatcgg
    23461 cagtccggcc cgatatccgt cgttgaagtg cgcgaccagg ccgtcgagaa agagatcgag
    23521 tgcgcgtgtc ggcccgggcg gcggtggcgc ggcggcggcg gcgcgcgcaa cctcgtagac
    23581 cccgccgccc agcgccaaac gacccgcgaa catcccggcc gacaatgcct gcaggtacgt
    23641 cgcacgggac agacccgcat caatggactc gagccggcga gcagccttca gcagcagaga
    23701 cggagcatcg ctgccccggc ttgcgacgaa cgcgagttca gcgccgatca tatcgatgcg
    23761 agcctgctga aggtcgctga gtggcccgca ccccgcggtg gccagcagct cctgcgcagc
    23821 gtcgagagct ccggcctcga ccttggcggt ggccgccgcc aacgcccggt cgctacgccg
    23881 agtcggatcg agcgtcaaca tcgcggcctg ttccaggaat gccgcggccg cggcaatgcc
    23941 cccgcgagcg cgggcacggt cagcgaaacg ttccagctcg gcggcgacct cgtcgtcggg
    24001 tgcgggcgca gcgtgcgccc gatgccaggc gcgtcgatcg ggatcatgtc ccggatcggt
    24061 gacctccgcg agcgccccgt gcaccatctg cctttcgtgc agcgacgcgg agcggtagac
    24121 cactgaccgc accagtggat gacggaagct gacccggctg ctgaattgga ccactcccgc
    24181 ctcgttggcg ggtgccgcgg cttccggacc gatccccaat cgtgcggcgg cccgccacac
    24241 gagcccggga tcagcagacg aatccgccgc ggcgaccagc agcagcagcc tg
//
LOCUS       DASSGQ010000330         5286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_753494,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000330 DASSGQ010000000
VERSION     DASSGQ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753494"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VFI55_14410"
     CDS             <1..764
                     /locus_tag="VFI55_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218227.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HET7075261.1"
                     /translation="CNQLPVHERHPYGGDLVYTAFSGSHQDAINKGLDAMKLDADAAD
                     VDMDDMLWQVPYLPIDPRDVGRTYEAVIRVNSQSGKGGVAYIMKADHGLALPRRLQIE
                     FSQVIQRIAEGTAGEGGEVTPKEMWDAFSEEYLAPILPLERMKQRVDAAEDDGGATSI
                     TATVKINGVETEISGTGNGPLAAFVDALGGVGFDVAVLDYSEHAMSAGDDAQAAAYVE
                     ASVSGKVVWGVGIAPSITTASLRAVVSAVNRASRN"
     gene            complement(769..1215)
                     /locus_tag="VFI55_14415"
     CDS             complement(769..1215)
                     /locus_tag="VFI55_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016882359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075262.1"
                     /translation="MDQENAPETTPLDERTEPVDGPVPDDAVSVRPTGGEERAQAPVA
                     PTEPADVTPERESSTEEMLFADADLTEMRGRWSAVQAAFVDDPKDCVQKADVLVSDLV
                     EQLTSSFARARSSLEEQWARGEQVSTEDLRLALMHYREFFERLLAV"
     gene            complement(1266..1841)
                     /locus_tag="VFI55_14420"
     CDS             complement(1266..1841)
                     /locus_tag="VFI55_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075263.1"
                     /translation="MPAWEWILIAAAVVIVVAALIIAASFVSGRTKSRRLQQHYGGEY
                     ERLVAETGGQKAAEKELTARERRRDKLEIVPLTPSALSDFTTRWQQVQTGFVDNPAAA
                     VGVADRLVTEVMRKRGYPVDDFDQRAADISVDHPQIVENYRAAHGIHLSQQHGDIDTE
                     QQREAFVHYRVLFTKLLETTTDHDASQEASA"
     gene            complement(2009..3001)
                     /locus_tag="VFI55_14425"
     CDS             complement(2009..3001)
                     /locus_tag="VFI55_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDDh family exonuclease"
                     /protein_id="HET7075264.1"
                     /translation="MNRVPWGLPASEPDGGWAVIDVETSGFRPGQARIISVAALGLDA
                     DGRVEQSVVSLLNPGVDPGPTHVHGLTAAMLEDQPQFADIVGDVSEVLRGRTLVAHNV
                     AFDYAFLAAEAELAGAELPVDTVMCTVELARRLDLGVDNLRLETLAAHWGVIQERPHD
                     AFDDALVLTGVLASALERARARDIWLPLRPVTRHRWPNGRVTHDELRPLKVLASRMPC
                     PYLNPGPYVRGRPLVQGMRVALATEVVRTHEELVERILHAGLAYSDAVDRETSLVVCN
                     DATQEQGKGYLARQLGVPVVSDAQFMDCVRAVAGGTSMEEFVDVTAVDDQLALF"
     gene            3074..4327
                     /locus_tag="VFI55_14430"
     CDS             3074..4327
                     /locus_tag="VFI55_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET7075265.1"
                     /translation="MLTTRARLALAAGAGARWASRVTGRGAGAMIGGLVAMTLDPSIL
                     RQLGAGRRTVVVTGTNGKSTTTRMTAAALATLGRVATNAEGANMDAGLVAALAADRGA
                     SLAALEVDEMHVPHVLDAVEAAVVILLNLSRDQLDRVGEINVIERTLRVGLARHPQAV
                     VVANCDDVLMTSAAYDSPNVVWVAAGGAWANDSVSCPRSGEVIVRDAPFQEGHWYSTG
                     ADFKRPNPQWWFDEDTLYGPDGLALPMRLALPGAVNRGNAAQAVAAAVALGADPAAAV
                     AAVSKVDEVAGRYRTFRIGAHEARILLAKNPAGWQEALSMVDKHAAGVVISVNGQVPD
                     GEDLSWLWDVRFEHFEKTALERPLVAAGERGTDLAVRLGYAGVEHTLVHDTLAAIASC
                     PPGSVEVVANYTAFLQLQRRLARHG"
     gene            4320..5033
                     /locus_tag="VFI55_14435"
     CDS             4320..5033
                     /locus_tag="VFI55_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET7075266.1"
                     /translation="MAEPVRIGLVLPDVMGTYGDGGNAVVLRQRLLLRGIAAEIVEIT
                     LADPVPESLDLYTLGGAEDYAQRLATKHLVRYPGVQQAAARGAPVLAICAAIQVLGHW
                     YETSAGERVDGVGLLDLTTSPQDRRTIGELVSKPLLAGLAQRLTGFENHRGGSVLGPA
                     AAPLGAVVKGAGNRADSPGNGFDGAVQGSVVATYMHGPCLARNPELADLLLSKVVGDL
                     PPLQLPEVDLLRRERLAAR"
BASE COUNT          828 a         1776 c         1825 g          857 t
ORIGIN      
        1 actgcaacca gctgccggtg cacgagcgtc acccctacgg cggggacctg gtctacaccg
       61 cgttctccgg cagccatcag gacgccatca acaaaggtct ggacgcgatg aagctggatg
      121 cagacgccgc agatgtcgat atggacgaca tgctctggca ggtgccctat ctgccgatcg
      181 acccgcgcga cgtcgggcgt acctacgagg cggtgatccg ggtcaactcc cagtccggca
      241 agggcggggt ggcctacatc atgaaggccg accacggcct ggcgctgccg cgacggctgc
      301 agatcgagtt ctcgcaggtg atccaaagga tcgctgaggg cactgcggga gaaggcggcg
      361 aggtcacccc caaggagatg tgggacgcgt tctccgagga gtacctggcg ccgatactgc
      421 cgttagagcg tatgaagcag cgcgtcgacg ccgcggagga cgacggcggc gccaccagca
      481 tcaccgccac cgtcaagatc aacggggtgg agaccgagat cagcggtacg ggcaatggcc
      541 cgctcgcggc gttcgtcgac gcgctgggcg gtgtcgggtt cgacgtcgcc gtcctggatt
      601 actccgagca tgcgatgagc gcgggcgacg acgcgcaggc ggcggcgtat gtggaggctt
      661 cggtgtccgg gaaggttgtc tggggcgtgg gcatcgcgcc gtcgatcacc accgcctcgc
      721 tacgggcggt ggtgtcggcg gtcaaccggg catcccggaa ctaacccgct atacggcgag
      781 cagcctctcg aagaattcgc ggtagtgcat cagcgcgagg cgcaggtctt cggtcgacac
      841 ttgctcgcca cgtgcccact gctcttcgag actcgagcgc gcgcgggcga aactggacgt
      901 gagctgctcg accagatcag acaccaggac atcagccttc tggacacaat ccttggggtc
      961 atcgacgaag gccgcctgca cggcggacca acgtccccgc atctccgtga ggtcagcgtc
     1021 tgcgaatagc atttcctccg ttgatgattc gcgttccgga gtcacgtcag cgggttccgt
     1081 cggtgcgacc ggggcctgag cccgctcctc gccgccagtc ggccgcacgc tcaccgcgtc
     1141 gtccggcacg gggccgtcca caggttccgt gcgctcgtca agtggtgttg tctccggcgc
     1201 attttcctga tccacgaccc gcgcctcgtc gggcgaatcg gtcagtgctt ggtgttcttg
     1261 cgttgtcatg cgcttgcctc ctgtgatgcg tcatggtctg ttgtggtttc gagcaatttc
     1321 gtgaacagca cccggtagtg cacaaacgcc tcccgctgtt gctcggtgtc gatatcgccg
     1381 tgctgctgcg agagatgaat gccgtgcgcg gcgcgataat tttcgacgat ctgcgggtgg
     1441 tctaccgaga tgtcggctgc ccgctgatcg aagtcgtcga ctggatagcc tcgtttgcgc
     1501 atcacctcgg tcaccaggcg gtcggcgacg ccgactgcgg ctgctgggtt atcgacaaac
     1561 ccggtctgca cctgctgcca gcgggtggtg aaatccgaca gggccgacgg cgtcagcgga
     1621 acgatctcga gtttgtctcg ccgacgttcc cgggcggtga gttctttctc cgccgccttt
     1681 tggcctcccg tttcggctac cagacgctcg tattcgccgc cgtagtgttg ctgcagtcgc
     1741 cgcgatttcg tgcgaccgct cacgaagctt gccgcgatga tcaaagcggc gaccacgatg
     1801 acgacggcgg ccgcgatcaa aatccattcc caagccggca tttcggctcc tctcgtactc
     1861 aagtcgaata gccggaccgg catcgatgca aacgtcgttt tgtgacgtgc aatacgtcgc
     1921 cgggttgcca tagcgcgccc gggggtgtca tggcaagcgc atcgcgggct cggagcccga
     1981 tgggtatttg tcgaaacgat gccgacgatc agaacagcgc gagctggtcg tccaccgcgg
     2041 tgacgtcgac gaattcctcc atgctcgtgc caccggcgac ggcgcgaacg cagtccatga
     2101 actgcgcgtc agataccacc ggcaccccta gctgccgggc gagatagccc ttgccctgtt
     2161 cctgggtggc gtcgttgcag accaccagcg aggtctcccg gtcgacggcg tcgctgtagg
     2221 ccagcccggc gtgcaggatc cgctcgacga gttcctcgtg ggtgcgtacc acctcggtcg
     2281 ccagcgccac ccgcatgccc tggaccagcg gcctgccccg aacgtagggt cccgggttga
     2341 gatacgggca gggcatccgc gaggccagca ccttgagcgg ccgcagctcg tcatgggtca
     2401 cccgcccgtt cggccagcgg tgccgggtca ccggacgcag cggcagccag atgtcgcggg
     2461 cgcgcgcccg ctcgagcgcc gacgcaagca cgcccgtcaa caccaatgcg tcgtcgaagg
     2521 cgtcgtgcgg ccgttcctgg atcacacccc agtgcgccgc gagcgtctcc agccgcagat
     2581 tgtcgacacc cagatccagc cgccgggcca gctcgaccgt gcacatgacg gtgtcgacgg
     2641 gaagttcggc gcctgcgagc tcggcctccg cggccaggaa tgcgtagtcg aacgcgacgt
     2701 tgtgcgcgac cagcgtgcgg ccgcgcagca cctcgctcac gtcgccgacg atgtcggcga
     2761 actgcggctg gtcctcgagc atggcggcgg tcagtccgtg cacgtgggtg ggccccgggt
     2821 ccaccccagg gttgagcagg ctgacgacgg actgttccac ccgcccgtcg gcgtcgaggc
     2881 cgagcgcggc aacgctgatg atccgcgcct gaccgggccg aaagcccgag gtttcgacgt
     2941 cgatgacggc ccatcccccg tccggctcgc tggccggcag accccagggc acccggttca
     3001 tgaactgagg atggcacgtc ggaccgacac gatccggtcg ctgcgccgcc gtgtcgccac
     3061 ataaactgcg cgcatgctga ccacccgggc acgtctcgct ctcgccgccg gggccggtgc
     3121 gcggtgggcg tcgcgggtca ccggacgcgg ggccggggcc atgatcggcg gcctggtggc
     3181 gatgaccctg gacccgtcga tcctccgcca gctcggtgcc ggccggcgca ccgtcgtcgt
     3241 caccggcacc aacggcaagt cgacgaccac ccggatgacg gcagccgcgc tggcgacgct
     3301 gggacgggtg gccaccaacg ccgaaggtgc caacatggat gccggcctgg tggcggcgct
     3361 tgccgccgac cgcggcgctt cgctggcggc gctcgaagtc gacgaaatgc acgtgccgca
     3421 cgtcttggat gcagtagaag ccgcggttgt cattttgctc aacctgtcgc gcgaccagct
     3481 ggaccgggtc ggcgagatca acgtgatcga acgcacgctg cgggtcggcc tggcccggca
     3541 cccgcaggcg gtggtcgtcg ccaactgcga cgacgtgctg atgacctcgg cggcctacga
     3601 cagccccaac gtggtgtggg tggcagcggg cggcgcgtgg gcgaacgact cggtcagctg
     3661 cccgcgcagc ggcgaggtga tcgtccgcga cgcccctttt caggaaggcc attggtattc
     3721 caccggcgcc gacttcaagc ggcccaatcc gcaatggtgg ttcgacgagg acacgttgta
     3781 tgggcccgac gggttggccc tgccgatgcg gttggcgctg ccgggcgcgg tgaatcgggg
     3841 caatgccgcc caggccgtgg ccgccgccgt cgctctcggc gccgatccgg cggcggcagt
     3901 ggcggccgtc tcgaaggtcg acgaggtcgc cggtcgttac cggaccttcc gtatcggcgc
     3961 acacgaagcg cggatcctgc tggccaagaa ccccgccggc tggcaggaag cgctgtccat
     4021 ggtggacaag cacgcggcgg gcgtggtcat ttccgtcaac gggcaggtgc ccgacggcga
     4081 ggacctgtcg tggctgtggg acgtgcgctt cgaacacttc gagaagacag ccttggaaag
     4141 gcccctggtg gccgccggcg aacgcggcac cgacctggcg gtgcggctgg gctacgcggg
     4201 cgtcgagcac acgttggtcc acgacaccct ggccgccatc gcatcctgcc cgcccgggag
     4261 tgtcgaagtc gtcgccaact acaccgcgtt cctccagctg cagcgtcggt tggctcggca
     4321 tggctgagcc ggtgcggatc gggctggtgc tgcccgacgt catgggcacc tacggcgacg
     4381 gcggcaatgc ggtggtgctg cgccaacggc tgctgctgcg gggcatcgcc gccgagatcg
     4441 tcgagatcac gctggccgac ccggtgccgg agtcgctgga cctctacacg ctgggcggcg
     4501 ccgaggacta cgcgcagcgg ctggccacca agcacctggt tcggtacccc ggcgtgcagc
     4561 aagcggcggc ccgcggtgcg cccgtgctgg cgatctgtgc ggccatccag gtgctcggac
     4621 attggtacga gacgtcggcg ggcgaacggg tcgacggcgt cggcctgctg gatttgacca
     4681 cctcaccgca agacaggcgc accatcggcg agctggtgag caagccgctg ctggccggcc
     4741 tggctcaacg cctgaccggt ttcgaaaacc accgcggcgg cagcgtgttg gggccggcgg
     4801 cggcaccgct gggggcggtg gtcaagggcg cgggcaaccg ggccgactcc ccgggcaatg
     4861 ggttcgacgg cgctgtgcag ggcagcgtgg tcgcgaccta catgcacggg ccgtgcctgg
     4921 cccgcaaccc ggagctggcc gacctgctgc tgagcaaggt cgtcggtgac ctgccaccgc
     4981 tgcagctgcc cgaggtggat ttgctgcgcc gagagcggct tgcggcccgc tgaggagtca
     5041 cagctgcgca ccaagtttcc tttgaggatc cgccaagggt cgtaaccgag ccttttctcg
     5101 acacaccgaa aaaggaaccc catgaccaac cccctgacca acccaaggac cacggccacc
     5161 aacgcggcgc gcatcgtcgg gctcgcggcc atgttgagcg cggccgccgg ctcgatggtt
     5221 gcgctcgccg ggacggccga cgcgaccctg gggatgcacg gcgacccggc cgccgccgcg
     5281 cgctac
//
LOCUS       DASSGQ010000331         9070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_722984,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000331 DASSGQ010000000
VERSION     DASSGQ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722984"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /locus_tag="VFI55_14440"
     CDS             complement(<1..1457)
                     /locus_tag="VFI55_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6541 family protein"
                     /protein_id="HET7075267.1"
                     /translation="MGLWFGTLIALFLLITPGTIVARITQLTWPIAVSVGPALTYGMV
                     ALAIIPFGALEIPWNGWTALATLAVCCVVATGLQIVFARYRDTEAEARGIGRWPAVTV
                     AVGVLVGALLIMWAGYRGLTHWQSIPSTWDAVWHANEVRFILDTGQASSTHMGELRNV
                     ETHQPLYYPSVFHALTAIYCQLTGAATTTAYTLNSLAASVWLFPTSAATFTWFLLRPI
                     STQWRTAGVSATAAALSASFTSVPYVEFGVAAMPNLAAYGVAIPTFILIASTLRHRDR
                     IPPAVLALVGVLSLHLTGGFIVILFLLVWWLFDAAWHPVRGRLADGLTLAAVLVPTLL
                     ILAPQFLGVLRQADIIAGHAFPSFKSVKQGIIDALLLHTRHLNDFPTQYGLVVLSAIG
                     MAIFLYKKIWWPPAVWVVLTVATIYSGAPFRNPFGRAIEEFSQFFYNDPRRLSAGVTL
                     VLTPMAAVALFALVAVVVAAAKRLTDRYTRLPAPV"
     gene            1587..2783
                     /locus_tag="VFI55_14445"
     CDS             1587..2783
                     /locus_tag="VFI55_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HET7075268.1"
                     /translation="MAYDVARVRGLHPSLGDGWVHFDAPAGMLIPDSVATTVSTAFRR
                     SASTTVGAHPSARRSAAVLEAARAAVADLFNADPAGVVLGADRAILLSSLAEASSSRA
                     GLGYEVIVSRLDDEANIAPWLRAAHRYGAKVKWAEVDIETGELPTWQWESLIGKSTRL
                     VAVTSASATLGTVTDLRAMTKLVHDVGGLVVVDHSAAASYRLLDVKETDADVLAVNAI
                     AWGGPPIGALVFRDPGLIGTFGSISTDPHATGPARLEVGVHQFGLLAGVVASIEYLAA
                     LDESARGSRRERLSVSMQSASGYLNRIFDYLMVALRSLPLVMVIGRPEVRIPVVSFAL
                     HDVPAERVVQRLADNGILAISNESSRALEVLGVNDVGGAVTVGLAHYSTTAEVDQLVR
                     ALASLG"
     gene            complement(2775..3761)
                     /locus_tag="VFI55_14450"
     CDS             complement(2775..3761)
                     /locus_tag="VFI55_14450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HET7075269.1"
                     /translation="MRAIVAESPDQMSWQDVPDVTAGPGEVLVKVAAAGVNRADVLQA
                     AGKYPPPPGASDIIGMEVSGVVAETAPDVTGWPVGKQVCALLAGGGYAEYVVVPSGQV
                     LPIPDGVDLVDAAGLPEVACTVWSNLVMTAHLSPGQLLLIQGGASGIGTHAIQVARAL
                     GARVAVTAGSADKLEFCRALGAEITINYRDEDFVARLREKTDGADVILDIMGAAYLDR
                     NIDALATDGQLVIIGMQGGVLGELNIGKLMAKRARVIGTTLRGRPVTGPNSKSAIVQA
                     VTASLWPTIAHGQIRPIIGARMPIQQAGYAHQQLVSGNVTGKIVLTVSPSST"
     gene            3798..3887
                     /locus_tag="VFI55_14455"
     tRNA            3798..3887
                     /locus_tag="VFI55_14455"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3832..3834,aa:Ser,seq:tga)
     gene            complement(3894..4916)
                     /locus_tag="VFI55_14460"
     CDS             complement(3894..4916)
                     /locus_tag="VFI55_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075270.1"
                     /translation="MRSGRFRQLLETPGPFVSVYFTDSEYADDAGAQPDGEWRALRNK
                     LERQGADETVIADIDDAVINLRPPMARGGRALVAGSTGVVLNEQLLGPPGEPIVRVSE
                     LPYIVPIIEHGVQQSHYLLAVVDPNGAFITTHADATRHSEMVNVDGSPKLTAVVDRVC
                     ELVHNASFDAVFVVSDAESRSDLLAALPERVRERATSLPIDVRRGGYDFEEIQRAIDT
                     TLVRQRLNVMDNAAERFTAEISRGSGLAAEGLDAVCSALHQGAVDTMLIGYEIDGLTV
                     VSDARLTTVAPNADALAGRGALPAKTLRADEALPLLAISVGASVVRIDERIAPADGIA
                     AVLRPA"
     gene            complement(5033..6268)
                     /gene="lipE"
                     /locus_tag="VFI55_14465"
     CDS             complement(5033..6268)
                     /gene="lipE"
                     /locus_tag="VFI55_14465"
                     /EC_number="3.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase LipE"
                     /protein_id="HET7075271.1"
                     /translation="MTPDGKVRVPADLDSVTAIGDEDHSEIGSAAVERIWQAARHWYQ
                     GGMQPAIQLCVRRHGRVVLNRAIGHGWGNAPADPPDAEQIPVTTDTPFCVYSAAKGIT
                     ATVVHMLVERGVFSLDDRVCHYIPTYTSHGKDRTTIRHVLTHSAGVPFPTGPRPDVKR
                     ADDHEYTQQKLGELRPLHRPGLAHMYHALTWGPLMREIVYAATGKEIRDILAAEILDP
                     LGFRWTNFGVAKEDLPLVAPSHATGRPLPPVVAQLFRKAIGGTVHEIIPYTNTPLFLT
                     TIIPSSNTVSTANELSRFAEIWCRGGELDGVRVMSPETLGGAVRQCRWLRPDLAMGLM
                     PARWGTGFILGTNRFGPFGRNAPFAFGHLGLINVAVWADPERGVAAGVISSGKPGRDP
                     ESRRYTALMDTIAAEMPIG"
     gene            complement(6338..7162)
                     /locus_tag="VFI55_14470"
     CDS             complement(6338..7162)
                     /locus_tag="VFI55_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HET7075272.1"
                     /translation="MGEAYESVTVETKDQIAQVTLVGPGKGNAMGPAFWSEMPEVFAK
                     LDADREVRAIVITGSGKNFSYGLDLPAMGGMFSPVMADGALARPRADFHAELLRMQKA
                     INAVADCRTPTIASVHGWCIGGALDLISAVDIRYASADARFSVREVRLAIVADMGSLA
                     RLPLILSDGHLRELALTGKDIDAARAEKIGLVNDVYEDADQSLAAAHATAAEIAANSP
                     LAVYGVKDVLDQQRTSAVSENLRYVAAWNAAFLPSKDLAEGISATFAKRPAQFTGE"
     gene            complement(7223..7696)
                     /locus_tag="VFI55_14475"
     CDS             complement(7223..7696)
                     /locus_tag="VFI55_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4334 domain-containing protein"
                     /protein_id="HET7075273.1"
                     /translation="MSLARKQFTEFKERGSAISDAELDEFWATLTPATIDGMIGEWKG
                     GEFVTGHKMNGKLEKAGWFGKTFKSAGDVQPLVCVDADGNKFSNVAMGKGEASLWLED
                     FRGEVTATMVYDGQPVHDHFKKIDDNAVMGIMNGKDVLDFQGGVGRYYYFYLERV"
     gene            complement(7706..8785)
                     /locus_tag="VFI55_14480"
     CDS             complement(7706..8785)
                     /locus_tag="VFI55_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7075274.1"
                     /translation="MTARLRPELVGLPVYVPGKTVPGSIKLASNETVFGPLPSVRAAI
                     EKATDRINRYPDSACVQLKAALAKHASSAGSADFAPEQVAAGCGSVSLCQQLVQIAAS
                     EGDEVIFGWRSFELYPPQVQVAGATAIKVPLTNHAYDLSAMLAAVTDRTRLIFVCNPN
                     NPTSTVVDPDALARFVEAAPPHILIAIDEAYVEYIRDGMLPDSLEMAHARSNVVVLRT
                     FSKAYGLAGLRVGYAVGHPDLITALDKVFVPFSVTSISQTAAIASLDAADELLARTDA
                     VVAERTRVTAGLREAGFCPPPSQANFVWLPLGPRTPDFAAQAADARIVVRPYGTDGVR
                     VTIGAPEENDAMLRFARDWIARTEP"
     gene            8875..8966
                     /locus_tag="VFI55_14485"
     tRNA            8875..8966
                     /locus_tag="VFI55_14485"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8909..8911,aa:Ser,seq:gct)
     gene            8987..9059
                     /locus_tag="VFI55_14490"
     tRNA            8987..9059
                     /locus_tag="VFI55_14490"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9020..9022,aa:Arg,seq:acg)
BASE COUNT         1499 a         2958 c         3102 g         1511 t
ORIGIN      
        1 cagaccgggg cgggcagccg ggtgtatcgg tcggtgagtc gtttggctgc cgcaaccacc
       61 accgcgacca acgcgaacaa tgcgaccgcc gccatcggcg tcagcaccaa cgtcacgcca
      121 gcgctgagcc gtcgcgggtc gttgtagaag aactggctga actcctcgat ggcgcggccg
      181 aacgggttac gaaacggtgc ccccgaataa atggtcgcca cggtcagcac cacccagacg
      241 gcgggcggcc accagatctt cttgtacaga aagatcgcca tgccgatggc ggacagcacc
      301 accaggccgt actgcgtcgg gaaatcgttg aggtggcggg tgtgcagcag cagcgcgtcg
      361 atgatgccct gtttcacgct cttgaagctc gggaatgcgt gccccgcgat gatgtcggcc
      421 tgcctgagca cgccgaggaa ctgcggcgcc aggatcagca gcgtcggcac cagcaccgcc
      481 gccagcgtca acccgtcggc gaggcgaccg cgcaccggat gccacgcggc gtcaaaaagc
      541 caccagacca acaagaacag gatgacgatg aacccgccgg tgaggtgcag cgacaggaca
      601 cccacgagtg ccagcacggc cggcggaatg cggtcgcggt gtcgcagggt cgaggcgatc
      661 agaatgaacg tgggaatcgc gaccccgtag gcggccaggt tgggcatcgc ggcgacgccg
      721 aactccacat aggggaccga cgtgaacgac gccgacagcg ccgcggcggt cgccgacacg
      781 ccggcggtgc gccattgggt gctgatcggg cgcaacagga accaggtgaa ggtggccgcg
      841 ctggtgggaa acagccagac cgatgcggcc agcgaattca gggtgtaggc ggtggtggtc
      901 gccgcccccg tcagctggca ataaatcgcg gtcagggcgt ggaacaccga cgggtaatac
      961 agaggctggt gggtttcgac attgcgaagc tcgcccatgt gggtcgacga cgcctggccg
     1021 gtgtcgagga tgaagcgcac ctcgttggcg tgccagaccg cgtcccatgt gctgggtatg
     1081 gactgccaat gcgtcaggcc gcgatagccc gcccacatga tcagcagagc gcccaccagc
     1141 acgccgacag cgacggtcac cgccggccag cgaccgatcc cgcgggcttc ggcctcggtg
     1201 tcgcggtagc gggcgaacac tatctgcaaa cccgttgcca ccacgcaaca caccgccagg
     1261 gtggccagcg ctgtccaacc gttccaaggg atttcgagcg caccgaacgg gatgatcgcc
     1321 agcgccacca tgccgtaggt cagcgccggg ccaaccgaaa cagcgatcgg ccaagttagc
     1381 tgggtgatgc gtgcgacgat cgtccctggc gtgatcagca agaacagtgc gatcaacgtt
     1441 ccgaaccaga ggcccacccg actagtatgg ctggccgggt gacctggctc gccgagcctc
     1501 cgtgcggctg gaaagtgtcg gccgttttcc cggcgcccgc tacggtcaca atttcgcgga
     1561 attgcggaag ctttaaggtg gctggtatgg cctacgacgt cgcccgggtg cgtggactgc
     1621 atccgtcgct gggtgacgga tgggtgcact tcgacgctcc ggccggcatg ctgatccccg
     1681 actcggtcgc gacgaccgtg tcgacggcgt ttcgcaggtc cgcatcgacc accgtcgggg
     1741 cgcacccgtc ggcacggcgc agcgccgccg tactcgaagc ggcccgtgcg gcggtggccg
     1801 atctgttcaa cgccgacccg gcgggcgtcg tgctgggcgc cgatcgcgca atcctgttgt
     1861 cgtcgctggc cgaagcgtcg tcgtcacgcg cgggtctggg ctacgaggtg atcgtcagcc
     1921 ggctcgacga cgaggccaac atcgccccgt ggctgcgggc ggcgcaccgc tacggcgcca
     1981 aggtgaagtg ggcggaagtc gacatcgaga cgggcgagct gccgacgtgg cagtgggaaa
     2041 gcctgatcgg gaagtcgacc aggctggtcg ccgtcacctc cgcgtcggcg acgctgggga
     2101 ccgtcacgga tctacgggcg atgaccaagc tggtgcacga tgtcggcggg ttggtggtgg
     2161 tcgaccattc cgcggcggcg tcgtaccggc tgctcgacgt caaggaaacc gacgccgatg
     2221 tgttggcggt caacgcgatt gcgtggggcg gcccgcccat cggggcgctg gtgttccgcg
     2281 atccgggcct gatcggcacg ttcggttcga tctcgaccga cccccacgcc accggcccgg
     2341 cgcgcctgga ggttggcgtg catcagttcg ggctgttggc cggcgtggtc gccagcatcg
     2401 aataccttgc cgcgctcgac gagtccgccc gcggcagccg acgcgaacgc ctgtcggtct
     2461 ccatgcaatc agccagcggg tatctgaacc ggatcttcga ctacctgatg gtggcgttgc
     2521 ggtcgttgcc gttggtgatg gtgatcggcc gaccggaggt gcggataccg gtggtcagtt
     2581 tcgcgctgca cgacgtgccc gccgagcggg tggtgcagcg gttggcggac aacggaattc
     2641 tggcgatctc caacgagagc tcgcgcgccc tcgaggttct cggtgtcaac gacgtcggcg
     2701 gcgccgtcac ggtcgggctg gcgcactact cgacgacggc cgaggtcgac cagttggtgc
     2761 gtgcgctggc gtcgctaggt tgacgacggc gagaccgtca gcacgatctt tccggtcacg
     2821 ttgcccgaca ccagctgttg atgtgcgtaa ccggcctgct ggatgggcat gcgcgcgccg
     2881 atgatcggcc tgatttggcc gtgggcgatc gtcggccaca gcgacgccgt taccgcctgc
     2941 acgatcgcgc tcttgctgtt cggcccggtc accggccggc cccgcagcgt ggtgccgatc
     3001 acccgtgccc gcttggccat caacttgccg atgttgagct cgcccaaaac gccgccctgc
     3061 atgccgatga tgaccagctg cccgtcggtg gccagcgcat cgatattgcg gtccaaatag
     3121 gcggccccca tgatgtcaag gatcacgtcg gcgccgtcgg ttttctcccg cagccgcgcc
     3181 acgaagtcct cgtcgcggta gttgatggtg atctcggcac ccagcgcgcg gcagaattcc
     3241 agcttgtccg ccgaccccgc ggtgacggcc acccgggcgc ccagcgctcg cgcaacctgg
     3301 atcgcgtggg ttccgatccc gctggcgccg ccctggatca gcagtagctg gcccgggctc
     3361 aggtgggcgg tcatcaccag gttcgaccac accgtgcagg cgacttccgg taacccggcg
     3421 gcgtcgacaa ggtccacgcc gtcggggatc ggcagcacct gaccggaggg gacgacgacg
     3481 tattcggcgt atccgccacc ggcgagcagc gcgcagactt gttttccgac aggccatccc
     3541 gtgacatccg gggccgtttc ggcgactacg ccggaaacct ccatgccgat gatgtcgctg
     3601 gctccgggcg gcggcggata tttaccggcg gcctgaagga catcggcgcg gttgacgccg
     3661 gccgcggcca ccttgaccaa gacctcgcca ggcccggcgg tgacgtcggg tacatcttgc
     3721 caggacattt gatcggggga ttcggcgacg atagcgcgca tgtcggcaac gctactagcc
     3781 ccgttaccct tgtcagcggt ggcgtggcag agcggcctaa tgcactcgcc ttgaaagcga
     3841 gagacggcta aaaccgtccg ggggttcaaa tccctccgcc accgccaaac gcgtcaggca
     3901 gggcgaagca ccgcggcgat accgtcggcc ggggcgatcc gctcgtcgat gcggaccacg
     3961 gacgccccca cggaaatcgc cagcagcggc agcgcttcgt cggcccgtag cgtcttggcg
     4021 gggagggcgc cccgcccggc cagcgcgtcg gcgttcgggg ccacagtcgt cagtcttgcg
     4081 tcggagacca cggtcaaccc gtcgatctcg tagccgatga gcatcgtgtc gaccgccccc
     4141 tgatgcaggg ccgagcagac ggcatcgagc ccctcggcgg ccagcccgga tccccggctg
     4201 atctcagcgg tgaagcgctc cgccgcattg tccatcacat tcagccgttg ccgcaccagc
     4261 gtcgtgtcaa tggcccgttg aatctcctcg aagtcgtatc cgccgcgccg gacgtcgatc
     4321 ggtagcgacg ttgcccgctc gcggacccgt tccggcagcg ccgccagcaa atccgagcgg
     4381 gactccgcgt cgctcaccac gaacaccgcg tcaaacgatg cgttgtgaac gagttcgcag
     4441 acgcggtcga ccacggcggt cagcttcggg gagccgtcaa cattgaccat ctccgagtgc
     4501 cgcgtggcat cggcgtgggt ggtgataaag gcgccattgg gatcgaccac ggccaagaga
     4561 tagtgcgact gctggacgcc atgctcgatg atcgggacga tgtacggcaa ttcggatact
     4621 cggacgatcg gctcgccagg cggcccgagc agttgctcgt tgagtaccac tcccgtggag
     4681 cctgcgacca gcgcgcgccc accgcgggcc atcggcggac gcaggttgat gacggcgtcg
     4741 tcgatgtcgg cgataacggt ttcgtcggcc ccctgccgct cgagtttgtt gcgcagcgcc
     4801 cgccattcgc cgtcgggctg agcgccggca tcgtcggcat attccgagtc cgtgaaatac
     4861 accgagacga aggggcccgg cgtctcaagt agctggcgga agcggcccga tcgcatggcg
     4921 gctacatata caccggccgg gcgccgctgg aaacgtcgct aagcgtcaag atttcgttaa
     4981 aaagcgcccc gcccggtctg atccacccgc agcgctcccg ccaccggggt ggttaaccga
     5041 tcggcatctc ggcggcgatg gtgtccatta gggcggtgta gcggcgggat tcggggtccc
     5101 ggccgggctt gccgctgctg atcacaccgg cggcgacgcc gcgctcgggg tcggcccaga
     5161 cggcgacgtt gatcaggccg aggtgaccga aggcgaacgg ggcgttgcgc ccgaacggtc
     5221 cgaaccggtt cgtgcccagg atgaacccgg tgccccagcg ggccggcatc agccccatcg
     5281 ccaggtccgg tcgcaaccac cggcactgcc tcaccgcgcc gcccagtgtt tccgggctca
     5341 tcacccgcac gccgtcgagt tcgccacccc ggcaccaaat ttcggcgaac cgtgacaact
     5401 cgttggcggt cgacacggtg ttggacgacg gaatgatggt ggtgagaaac agcggggtgt
     5461 tggtgtacgg gatgatctcg tgcacggttc cgccgatcgc cttgcggaag agttgggcga
     5521 ccaccggcgg cagcggccgt ccggtggcat ggctcggtgc gaccaacggc aggtcttctt
     5581 tggcgacgcc gaagttggtc cagcggaagc ccagcgggtc caggatttcg gcggccagga
     5641 tgtcgcgaat ctccttaccc gtggccgcgt agacgatctc gcgcatcagc ggaccccagg
     5701 tcaacgcgtg gtacatgtgg gccagccccg gtcggtgcaa cggccgcagt tcgccgagct
     5761 tctgttgggt gtattcgtgg tcgtcggcgc gcttgacgtc cggcctgggg ccggtcggaa
     5821 acgggacgcc ggcgctgtgg gtcaacacgt gccggatcgt ggtgcgatcc ttgccgtgac
     5881 tggtgtaggt cggtatgtag tggcagaccc ggtcgtccag ggagaacacc ccgcgctcca
     5941 cgagcatgtg caccacggtg gcggtgatgc ccttggccgc cgagtacacg cagaacgggg
     6001 tgtccgtggt gaccgggatt tgctctgcgt cgggtgggtc ggccggggcg ttgccccagc
     6061 cgtggccgat cgcgcggttg agcacgacgc gcccgtgtcg ccggacgcat aactggatcg
     6121 ccggttgcat gccgccctgg taccagtgcc gcgcggcctg ccagatccgt tcgacggcgg
     6181 cgctgccgat ctcagagtgg tcctcgtcgc cgatcgccgt cacggagtcc aagtcggccg
     6241 ggacgcggac cttgccgtcg ggcgtcattg cgccatcgcc gttacgggtg aagttggaca
     6301 ccccagcgag ggtacgtgcc gccgggtggc aggtgcgcta ctcgccggtg aactgcgccg
     6361 gacgcttggc gaacgtcgcc gagatgccct cggccaaatc cttcgacggc aagaaggctg
     6421 cgttccaggc ggcgacgtag cgcaagttct ccgacacggc cgacgtgcgt tgctgatcca
     6481 acacgtcctt gacgccgtag accgccaggg gcgagttcgc ggcgatctcg gcggcggtcg
     6541 cgtgggccgc ggccagcgac tggtcggcgt cctcgtacac gtcgttgacc aggccgatct
     6601 tctccgcgcg cgccgcgtcg atgtccttgc cggtcagcgc gagttcccgc aggtgtccgt
     6661 cgctcaggat cagcggcagc cgggccaggc tgcccatgtc ggcgacgatg gcgagtctga
     6721 cttcgcgcac cgagaacctc gcgtccgcgc tggcgtagcg gatgtccacc gccgagatca
     6781 ggtcgagggc accaccgatg caccagccgt gcaccgacgc gatcgtgggg gtgcggcagt
     6841 cggcgacggc gttgatcgcc ttctgcatcc gcagcagctc ggcgtggaag tcggcgcggg
     6901 ggcgtgccag cgcgccgtcg gccatcaccg gggagaacat gccgcccatc gcgggcaagt
     6961 ccagcccgta gctgaagttc ttgcccgagc ccgtgatgac aatggcccgc acctcgcggt
     7021 cggcgtccag ctttgcgaac acctctggca tctccgacca gaacgccggg cccatcgcgt
     7081 tgcccttgcc cgggccgacc agcgtcacct gcgcaatctg gtctttggtt tcgacggtga
     7141 cggattcgta tgcttcaccc atatccagac cgtagcgtgg cgaagacgct ccctgatttg
     7201 cgacccctga gcgggacggc ggctacacgc gctccaggta gaagtagtag tagcggccca
     7261 ccccgccctg gaagtccagg acgtccttgc cgttcatgat gcccatcacc gcgttgtcgt
     7321 cgatcttctt gaagtgatcg tgcacgggtt ggccgtcata gaccatcgtg gcggtgactt
     7381 cgccgcggaa gtcctccagc cacaggctgg cctcgccctt gcccatcgcc acgttggaga
     7441 acttgttccc gtcggcgtcc acacagacca acggctgcac atcgccggcc gatttgaagg
     7501 tcttgccgaa ccagccggcc ttctccaact tcccgttcat cttgtggccg gtgacgaact
     7561 cgccgccctt ccactcgccg atcatcccgt cgatggtcgc gggcgtaagc gtggcccaga
     7621 actcgtcgag ctcagcgtcg gaaatggcgc tgccgcgttc cttgaactcg gtgaactgct
     7681 tgcgggccaa gctcattggc tctcctcaag gttccgtgcg ggcaatccaa tcgcgggcaa
     7741 accgcagcat ggcgtcgttt tcctccggcg ctccgatggt gacccggacg ccgtccgtgc
     7801 cgtacgggcg gaccacgatg cgcgcatcgg cggcctgtgc cgcgaagtcc ggcgtgcgag
     7861 ggcccagcgg cagccagacg aagttggcct gtgacggtgg cgggcagaat ccggcttcac
     7921 gcagacccgc ggtgactcgg gtgcgctcgg cgaccacggc gtcggtgcgg gcgagcaatt
     7981 cgtcggcggc gtccagcgac gcgatcgcgg cggtctgcga aatgctcgtc accgaaaacg
     8041 gcacaaagac tttgtccagc gcggtgatca ggtcaggatg gccgaccgcg tagccgacgc
     8101 gtagaccggc cagcccgtac gccttcgaaa aggtccgcaa cacaacgaca ttgcttcggg
     8161 cgtgggccat ctccaggctg tcgggcaaca tgccgtcgcg aatgtattcg acgtaggcct
     8221 cgtcgatggc gatgaggatg tgcggcggtg ctgcttcgac gaagcgggcc agtgcgtcgg
     8281 ggtcgacgac ggtggacgtc ggattgttgg ggttgcagac gaagatcagt cgggtgcggt
     8341 cggtgacggc ggccagcatc gcggagaggt cgtaggcgtg attggtcaac ggcactttga
     8401 tggcggtggc gccggcgacc tgaacctgcg gcggataaag ctcgaagctg cgccacccga
     8461 agatgacctc gtcgccctcc gaggcggcga tttgaaccaa ctgctggcac aggctgaccg
     8521 aaccgcatcc ggcagcgacc tgctcagggg cgaagtccgc ggaacccgcc gaactggcgt
     8581 gcttggccag cgcggccttg agctgcacgc aggcactgtc ggggtaccgg ttgattcggt
     8641 cggtggcctt ttcgatggcc gcgcggacgc tggggagcgg gccgaagacc gtctcgttgc
     8701 tggccagttt gatcgaaccc ggcaccgtct tgccaggcac atacactggc agcccgacca
     8761 actcgggtcg caggcgggcg gtcacttcga cagcatatgt cggcgctgtg tacgctatgc
     8821 ctccggcggt tcagagacgg ttggaagggg tcgggtaccc tcagaaagtt caatggaggc
     8881 gtgccagagc ggccgaatgg ggctcactgc taatgagttg tcccccttca aggggaccgg
     8941 aggttcaaat cctctcgcct ccgccacgca agacaactga acacatgcgc ccgtagctca
     9001 acggatagag catctgacta cggatcagaa ggttgggagt tcgaatctct tcgggcgcgc
     9061 tgcaggtcat
//
LOCUS       DASSGQ010000332        12096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_298133,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000332 DASSGQ010000000
VERSION     DASSGQ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12096
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298133"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFI55_14495"
     CDS             complement(<1..363)
                     /locus_tag="VFI55_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003914920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7075275.1"
                     /translation="MALASGATFAGYIVSRRLGSGATGAVYLVQDPRSVRWRALKVLS
                     PAMSDDAEFRRRFRAETPLAANLRHPNIVEVHERGEFEGQLYVVMEYVEGINAARLIA
                     DRFPAVSPAGEALAIVTAV"
     gene            complement(419..1489)
                     /locus_tag="VFI55_14500"
     CDS             complement(419..1489)
                     /locus_tag="VFI55_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7075276.1"
                     /translation="MRVRGRALPDETEVELWIVDGRISTEPVAGADTVFDGGWILPGL
                     VDAHCHVGLGDPGEIPLDEAIGQAETERDVGALLLRDCGVPTDTRSLDDHDDLPRIIR
                     AGMHLARPKRYQAGFSVELEDESQLPAAVAEQARRGDGWVKLVGDWIDRGVGDLAPLW
                     SDDVLKAAIDTAHAHGARVTAHVFSEDALPGLIGAGIDCIEHGTGLTDDTIALMLEHG
                     TALVPTLINIVENFPRFADGAARYPAYAAHMRDLHARCPGRVAAAVDAGVPVYAGSDA
                     GSVIAHGRIADEVEALKGVGMSPTRALGAACWDARRWLGRPGLDHGASADLVCYSHDP
                     RLGPAVLSRPDLVILRGKAFRT"
     gene            complement(1503..3062)
                     /gene="ffh"
                     /locus_tag="VFI55_14505"
     CDS             complement(1503..3062)
                     /gene="ffh"
                     /locus_tag="VFI55_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET7075277.1"
                     /translation="MFESLSDRLTGALQGLRGKGRLTDADIEATTREIRLALLEADVA
                     LPVVRAFVGRIKERARGAEVSGALNPAQQVVKIVNEELIGILGGETRQLAFAKTPPTV
                     VMLAGLQGSGKTTLAGKLAAWLRGQGHTPLLVACDLQRPAAVNQLQVVGQRAGVPVFA
                     PHPGASPESGPGDPVAVASAGLAEARAKHFDVVIVDTAGRLGIDDELMAQAAAIRDAV
                     DPDEVLFVLDAMVGQDAVATAEAFREGVGFTGVVLTKLDGDARGGAALSVREVTGVPI
                     LFASTGEKLEDFDVFHPDRMSSRILGMGDVLSLIEQAEQVFDAEQAEATAAKIGSGEL
                     TLEDFLEQMLTIRKMGPIGNLLGMLPGAGQMKEALAQVDDRQLDRLQAIIRGMTPQER
                     ADPKIINASRRLRIANGSGVTVAEVNQLVDRFFEARKMMSSMMGSMGLPGLSRRSATR
                     KSAKGKGGKKGKKAGRGPTPPKVRSPFGPGMPAMPPGFPDLSQMPEGLNELPPGLADF
                     DLSKLKFPGKN"
     gene            3306..5063
                     /locus_tag="VFI55_14510"
     CDS             3306..5063
                     /locus_tag="VFI55_14510"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7075278.1"
                     /translation="MQSGSDTGSHVQNGAGFSGGGVPLRGWQRRALVKYLAAQPRDFL
                     AVATPGSGKTTFALRVAAELLSHRAIEQITVVVPTEHLKVQWAMAAARHGVALDPRFS
                     NTGGQTSTEYHGVMVTYAQVASHPTLHRVRTEQRKTLVIFDEIHHGGDAKTWGEAIRE
                     AFSDATRRLALTGTPFRSDDSPIPFVTYASGADGLLRSQADHTYGYAEALADGVVRPV
                     VFLAYSGQARWRDSAGEEHAARLGEPLSAEQTARAWRTALDPAGEWMPAVIAAADERL
                     RQLRTHVPDAGGMIIASDQTAARAYAALLTRATSEAPTVVLSDDPGSSARITEFAEST
                     SRWLVAVRMVSEGVDVPRLAVGIYATSASTPLFFAQAIGRFVRSRRPGETASIFVPSV
                     PNLLQLASELEAQRNHVLGQPHRESGDDPLDGDPAVRTQTEKGETDPGFISLGADAEL
                     DQVIFDGSSFGTAAPAGSDEEADYLGIPGLLDADQMRALLHRRQDEQLKKRARLQEHA
                     KPGALSISMHGQLRDLRRELNNLVSATHHRTGKPHGWIHSELRRRCGGPPIAAATREQ
                     LQARIEALRQLNSEQTRGS"
     gene            complement(5060..7549)
                     /locus_tag="VFI55_14515"
     CDS             complement(5060..7549)
                     /locus_tag="VFI55_14515"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HET7075279.1"
                     /translation="MKPHPSDPSGTSGSLEKTGTGSASGASDLAASRRQLLSEAVELD
                     AAGLRQAWLELHESWLTARAAEIGITDDSGFAIVGIGGLGRRELLPYSDLDLMLVHDN
                     KPADVLERVADRLWYPLWDANVRLDHSVRTVSGALSVANTDMVVALGMLDARHIAGEA
                     RLSTELIGGARRQWRTAIRSRMDELVEMTHARWLRCGRISQRAEPDLKSGRGGLRDVQ
                     LLDALAVAQLIDRHGMSNPNVPAGSLDAAYLTLLDVRTELHRVSGRGRDLLLAQFADE
                     ISASLNFGDRFDLARRLSGAARTIAYHAVTGLRTAQNALPRRGVSALVRRPKRRPLDE
                     GVVEYANEIVLARDASPETDVGLVLRVAAAAADNGLPIGAGTLSRLAASAPEMPIPWP
                     SEALDDLLVLLFAGPTALGTIEALDRTGLWGRLLPEWDAIRDLPPRDVAHKWTVDRHV
                     VETAVNAAPLATRVARPDLLALGALLHDIGKGRGVDHSVLGAELALEIAPRLGVAPAD
                     VQFLSKLVRHHLLLPIVATRSDLNDPKTIEGVAEALGGDPHLLEVLHALTEADSKATG
                     PGVWSEWKASLIADLVRRCRMMMAGEPLPRADPTTPHYLALAADHGVHVEITPGDSAR
                     TYHVVMVAPYQRGLTSKAAAVLALNSLGVHSATVNSHEGVAITEFVVSPHFGSPAAAG
                     LLRQQFIGALAGDIDVLGMVKKRASEAASSASARAGEVVAGVPVTRSTAPPRIVWLDT
                     AAEGQLVLEVRAMDRPGLLALLTRALENAGVDIAWAKVNTFGSTAADVFCIELPTWAS
                     EESGDAVSTAQAAVEKQLLAVLGGPGEVTLS"
     gene            complement(7604..7942)
                     /gene="glnB"
                     /locus_tag="VFI55_14520"
     CDS             complement(7604..7942)
                     /gene="glnB"
                     /locus_tag="VFI55_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HET7075280.1"
                     /translation="MKLITAIVKPFTLDDVKTSLEDAGVLGMTVSEVQGYGRQKGHTE
                     VYRGAEYSVDFVPKVRIEVVVDDSIVDKVVDSIVRSARTGKIGDGKVWVSPVETIVRV
                     RTGERGTDAL"
     gene            complement(7939..9321)
                     /locus_tag="VFI55_14525"
     CDS             complement(7939..9321)
                     /locus_tag="VFI55_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003913414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET7075281.1"
                     /translation="MLASSALVLLMTPGLAFFYGGMVRARSVLNMLMMSISAMGVVTV
                     LWVLYGYSIAFGDDVGNVMGKPTDFWGLKGLIGVNSVAADPTKGVTAVDIPLAGTLPA
                     TVFVAFQLMFAIITVALISGAVSDRLKFSAWLVFAGLWATFVYFPVAHWVFAFDGFAS
                     EHGGWIANKLHAIDFAGGTAVHINSGVAGLVLAIVLGKRRGWPTTLFRPHNLPFVMLG
                     AGLLWFGWYGFNAGSATSANGVAGSTFMTTTVATGAAMLSWMLTERIRDGKATTLGAA
                     SGIVAGLVAITPSCSSVNVLGALVIGFVAGALCAVACGLKFKLGFDDSLDVVGVHLIG
                     GLAGTLMVGLLAAPESPAINGVTGVSKGLFYGGGWSQLERQAVGAFSVLFYSATVTLI
                     IALILKYTIGLRLGAEEESSGIDEAEHAESGYDFALVTGSVLPPRALVEETRNGQEDI
                     VGAKLEAEHK"
     gene            complement(9512..10882)
                     /gene="ftsY"
                     /locus_tag="VFI55_14530"
     CDS             complement(9512..10882)
                     /gene="ftsY"
                     /locus_tag="VFI55_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET7075282.1"
                     /translation="MSQGLWIAIAVVAALVVIAALVLGLVRYRRRRIRLTPPSQGPQP
                     SAIDRSGGYTASSGIMFSRTPTVEDRIDTSGLPAVGDDATVPRDAPRRTISDVQLPEP
                     EVVAPPPAAPTPQAPPAAPPAPAVPEAPPAPEAPEVPDAPETAEIDAIAPPEGRLERL
                     RGRLARSQNALGRSMLGLIGGGDLDEDSWQDVEDTLLIADLGPVATASVILELRARLA
                     SGNVRTEADAKAVLRDVLIKELQPGMDRSIRALPHADHPSVLLVVGVNGTGKTTTVGK
                     LARVLVADGRRVVLGAADTFRAAAADQLQTWAARVGAEVVRGPEGADPASVAFDAVDK
                     GIASGADVVVIDTAGRLHTKVGLMDELDKVKRVVTRRAAVDEVLLVLDATIGQNGLAQ
                     ARVFADVVEITGAVLTKLDGTAKGGIVFRVQQELGVPVKLVGLGEGPDDLAPFEPAAF
                     VDALLG"
     gene            complement(10962..>12096)
                     /locus_tag="VFI55_14535"
     CDS             complement(10962..>12096)
                     /locus_tag="VFI55_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7075283.1"
                     /translation="EARWRAQQARAARRRAAAVAAAVADAGRLLAGRLDRVVDATSRI
                     RDALSAERQGRSTAMTAVRDEVHALGTRVATLTDSLHRDEVANAQAALRIEQLEQMVL
                     EQFGMAPADLVAEYGPQVPLPPTELEVAEFEQARERGEQVTAPAPMPYDRVTQERRAK
                     RAERELAELGRVNPLALEEFAALEERYNFLSTQLEDVKGARKDLLGVVADVDDRILQV
                     FSDAFADVEREFRDVFASLFPGGEGRLRLTDPSDMLATGIEVEARPPGKKITRLSLLS
                     GGEKALTAVAMLVAIFRARPSPFYIMDEVEAALDDTNLRRLIGLFEQLRDRSQIIIIT
                     HQKPTMEVADALYGVTMQGDGITAVISQRMRGQQVGRLVASSS"
BASE COUNT         1879 a         4298 c         4071 g         1848 t
ORIGIN      
        1 gacggcggtc acgatcgcaa gcgcctcgcc cgccggtgag accgccggga atcgatcggc
       61 aatcagccgc gcggcgttga tcccttcgac gtattccatg acgacgtaca gctggccctc
      121 gaactcgccg cgctcgtgca cctcgacgat gttcgggtgg cgaagatttg ccgcgagcgg
      181 ggtttccgcg cggaatcgtc ggcggaattc agcgtcgtcc gacatcgccg gcgaaagaac
      241 tttcaatgcg cgccagcgca ccgaccgggg atcttggacg aggtagaccg cgccagtcgc
      301 tccagaaccc agcctccgcg acacgatgta accggcgaaa gtcgcgccgc tggccaacgc
      361 catcccgcga tactatccgc cgccgctgcg ccgcgcctgc ccggtgcccg ggccgtccct
      421 aggtccgaaa cgccttgccg cgcagtatca ccagatcggg gcggctcagg accgccggac
      481 ccagccgcgg atcgtgggag tagcacacca ggtcagccga cgctccgtgg tcaagaccgg
      541 gccggccgag ccagcgacgg gcatcccagc acgcggcgcc caacgccctg gtggggctca
      601 tcccgacgcc cttgagcgcc tcgacctcgt cggcgatccg cccgtgcgcg atcacgctgc
      661 ctgcgtcgct gccggcgtat accggcacgc ccgcatccac cgctgcggcc acccgccccg
      721 ggcagcgggc gtgcaggtcg cgcatgtgtg ccgcgtaggc cgggtaacgc gcggcgccgt
      781 cggcgaagcg cgggaagttc tcgacgatgt tgatcagggt gggaaccagg gcggttccgt
      841 gctcgagcat cagggcgatg gtgtcgtcgg tcagcccggt gccgtgttcg atgcagtcga
      901 tgccggcgcc gatcaggccg ggcagcgcgt cttcgctgaa gacgtgcgcg gtgacgcgag
      961 cgccgtgggc gtgtgcggta tcgatggcgg ccttgagaac gtcgtcggac cacagcgggg
     1021 ccaggtcccc gacgccgcgg tcgatccagt cgccgaccag cttgacccag ccgtcgccgc
     1081 gcctcgcctg ttcggccacc gctgccggca gctgcgactc gtcctccagc tcgacggaaa
     1141 agccggcctg atagcgcttg ggtcgggcca ggtgcattcc ggcccggatg atgcggggca
     1201 gatcgtcgtg gtcgtcgagg ctgcgagtgt cggtaggtac accgcagtca cgcagcagca
     1261 gcgcgccgac gtcgcgttcg gtctcggcct ggccgatcgc ctcgtcgagc ggaatctcgc
     1321 cggggtcgcc gagcccgacg tgacagtgcg catcgaccag accgggcagg atccagccgc
     1381 cgtcaaaaac ggtgtcggct ccggctaccg gttcggtgct gatgcggccg tcgacgatcc
     1441 acagttcgac ctcggtctcg tcgggcagcg cccgacctcg cacgcgcatg cgcacagcgc
     1501 ggctagttct tgccggggaa cttcagcttg gacaggtcga agtcggccag cccgggtggc
     1561 agctcgttga ggccttcggg catctgcgac agatcgggga atccgggcgg catggcgggc
     1621 atgccgggtc cgaacgggct ccggaccttg ggcggcgtgg gtccccgtcc cgccttcttg
     1681 cccttcttcc cgcccttgcc tttggcgctc ttgcgcgtcg cggacctgcg gcttagtcca
     1741 ggcaggccca tcgagcccat catcgacgac atcatcttgc gggcttcgaa gaagcggtcg
     1801 accagctggt tgacctcggc gaccgtcacc cccgagccgt tggcgatgcg cagccgacgc
     1861 gacgcgttga tgatcttggg gtcggcccgc tcttgtgggg tcatcccgcg aatgatggcc
     1921 tgcagccggt cgagttgcct gtcgtcgacc tgggccagtg cttctttcat ctggccggcg
     1981 ccgggcagca tgcccagcag gttgccgatc gggcccatct tgcggatggt gagcatctgc
     2041 tcgaggaagt cctccagggt cagctcgccg gacccgatct tggcggcggt ggcctcggcc
     2101 tgctcggcgt cgaagacttg ctcggcctgt tcgatgaggc tcagcacgtc gcccataccc
     2161 aggatgcggc tggacatgcg gtcggggtgg aagacgtcga agtcctccag cttctcgccg
     2221 gtggatgcga aaaggattgg cacgccggtc acttcgcgga ccgacaacgc ggcgcccccg
     2281 cgggcgtcgc cgtccagctt ggtcagcacc acgccggtga agccgacgcc ctcgcggaag
     2341 gcttcggcgg tggcgacggc gtcctggccg accatcgcgt ccaagacgaa cagcacctcg
     2401 tcggggtcga cggcgtcccg gatggccgcg gcctgcgcca tcagctcgtc gtcgatgccc
     2461 aagcggcccg cggtgtccac gatgacgacg tcgaagtgtt tggcgcgggc ctcggcaagc
     2521 ccggctgacg cgacggcgac cgggtcgccc gggccggact cgggtgacgc tccgggatgc
     2581 ggcgcgaaca ccggcacccc ggcacgctgc ccgacgactt gcagctggtt gacggccgcc
     2641 ggccgttgca ggtcgcaggc caccagcagc ggcgtgtgtc cctggccgcg cagccaggcg
     2701 gccaatttgc cggccagggt ggtcttaccg gaaccctgca gaccggcgag catcaccacg
     2761 gtcggcggcg tcttggcgaa cgccagctgg cgggtctcgc cgcccaggat gccgatgagt
     2821 tcctcgttga cgatcttgac gacctgttgc gccgggttga gggcgccgga gacctcggcg
     2881 ccccgggcac gctccttgat ccgacccacg aacgcgcgca ccacgggcag cgcaacgtcg
     2941 gcttccagca gcgccagccg tatttcgcgg gtggtggcct cgatatcggc gtcggtcagt
     3001 cggcccttgc cgcgcagccc ctgcagggca ccggtcaacc ggtcggacag cgattcaaac
     3061 accccgtcag cctaatggtg ccgcgcgtgc gcggcttccg gtgtccgagc cgggcgcggc
     3121 gggtcaccac gcacggtagc cggccccgag tcgctgctgc gccgaaaagc gttggcatgc
     3181 tacatttcac acccgccatc ggccgcgtcg gccgtgtcgc ggacagcaag attttcgcac
     3241 cgccggcacc cgcatgtgcc ggtgaacggg tggcgcgcgg agcgtctacg gggggagctg
     3301 tgtcgatgca atcgggcagc gacaccggct cgcacgtcca gaacggtgcc ggcttcagcg
     3361 gcggcggcgt gccgctgcgc ggctggcagc gccgagcact ggtgaagtac cttgccgccc
     3421 agccgcgtga tttcctggcc gtagccactc cgggatccgg aaagaccacg ttcgcgctgc
     3481 gggtggcggc cgagctgctc agccatcgcg ccatcgaaca aatcaccgtt gtggtgccca
     3541 ccgagcatct caaggtccag tgggcgatgg ccgctgcccg ccacggtgtc gccctggacc
     3601 cgaggttctc caacaccggc ggccagacct caacggagta tcacggcgtg atggtcacct
     3661 acgcccaggt cgcctcgcat ccgacgctgc accgggtgcg caccgagcag cgcaagaccc
     3721 tggtcatctt cgatgagatc caccacggcg gtgacgccaa gacgtggggc gaggccatcc
     3781 gggaggcctt cagcgacgcc actcgccgac ttgccctgac gggcacaccc tttcgcagcg
     3841 acgacagccc gatcccgttc gtcacctacg cgtccggcgc cgacgggctg ctgcgttcgc
     3901 aagccgatca cacctacggc tatgccgaag ctctggccga cggtgtggtc cggccggtgg
     3961 tcttcctcgc ctactcgggt caggcgcgct ggcgggacag cgccggcgaa gagcacgcgg
     4021 caagattggg tgagccgctg tccgccgagc agaccgcgcg ggcgtggcgc acggcgcttg
     4081 accccgcggg cgaatggatg cccgcggtga tcgcggccgc cgacgagcgg ctccgccagc
     4141 tgcggacgca cgtgcccgac gccggcggca tgatcatcgc ctccgaccag acggcagcgc
     4201 gcgcgtatgc cgccctgctg acgagggcga cgtcggaggc gccgacggtg gtgctctccg
     4261 acgacccggg atcgtccgcc cgcatcaccg aattcgccga gagcaccagt cgctggctcg
     4321 tcgcggtgcg catggtctcc gaaggcgtcg acgtgccccg gctggcggtg ggaatctacg
     4381 ccaccagcgc ctcgacgccg ctgttcttcg cgcaggccat cggccgattc gtgcggtcgc
     4441 gccggccggg ggaaacggcg agcatcttcg tgccgtcggt gcccaacctg ctgcagctag
     4501 ccagcgagct ggaggcccag cgcaatcacg tgctcggcca gccacaccgc gaatccggcg
     4561 acgaccccct cgacggcgac cccgccgtgc ggacccaaac cgaaaagggc gaaacagacc
     4621 cgggattcat ctcgttggga gccgacgccg agctggatca ggtcatcttc gacggctcct
     4681 cgttcggtac cgccgccccg gccggaagcg acgaggaagc cgactacctc ggtatccccg
     4741 gcttgctcga cgccgaccag atgcgcgccc tgctgcaccg acgccaggac gagcagctca
     4801 aaaagcgagc ccggctgcag gaacacgcca aacccggcgc cctgtcgata tcgatgcacg
     4861 gccagctgcg cgatctgcgg cgcgagctca acaacctggt gtcggccacc caccaccgca
     4921 ccggcaaacc acacgggtgg attcacagcg agctgcgccg tcgctgcggc ggtccaccga
     4981 tcgcggcggc gacccgcgaa cagctgcagg cccgcatcga ggcgctgcgg cagctgaatt
     5041 ccgagcagac gcgagggagc tagctaaggg tgacctctcc gggcccgcct agcaccgcca
     5101 gcagctgctt ctccacggcc gcttgtgcgg ttgacaccgc gtcgcccgac tcttccgagg
     5161 cccaggttgg cagctcgatg cagaacacgt cggccgccgt cgacccgaac gtgttgacct
     5221 ttgcccaggc gatgtccact cccgcgttct ccagcgcccg ggtcagcaac gccagcaggc
     5281 cgggccgatc catggcacgg acttccagga ccagctgccc ttcggcggcg gtatcgagcc
     5341 acacgatgcg cggcggggcg gtggagcgtg tcacggggac gcccgccacc acctcccccg
     5401 cccgagcgga cgccgaactg gccgcctcgc tggcacgctt cttgaccatg cccaggacat
     5461 cgatatcgcc ggcgagggca ccgatgaact gctgacgcag caaaccggcc gccgccggcg
     5521 agccgaagtg cggtgacacc acgaactcgg tgatcgcgac gccttcgtgg ctgttcaccg
     5581 tggccgaatg aacccccagt gagttcagcg ccagcacggc ggcggctttc gacgtcaatc
     5641 cgcgctggta cggggcgacc atcacgacgt gataggtgcg cgcactgtca cccggcgtga
     5701 tctcgacgtg cacgccgtgg tcggcggcca gtgcgagata gtggggcgtg gtgggatcgg
     5761 cccgcggcag cggctcgccc gccatcatca tccggcaacg gcgcaccagg tcagcgatga
     5821 gcgacgcctt ccattcgctc cacaccccgg ggccggtggc cttcgagtcc gcctcggtca
     5881 gcgcgtgcaa cacctcgagc aggtgcggat ccccgccgag agcttccgcg acgccttcga
     5941 tggttttggg gtcgttgagg tcgctgcggg tggccacgat gggcagcagc aggtggtggc
     6001 ggaccagttt cgacaggaac tgcacgtccg cgggcgctac gcccagccgg ggggcgatct
     6061 cgagagccag ctcggccccg agcacgctgt gatcgacacc tctgcccttg ccgatgtcgt
     6121 gcagcagcgc accgagggcg agcagatcgg gccgcgccac ccgggtggcc agcggcgccg
     6181 cgttgaccgc ggtctcgacc acgtgacggt ccaccgtcca cttgtgcgcg acgtcgcggg
     6241 gcggaaggtc gcggatcgcg tcccactcgg gcagcaaccg tccccatagc ccggtgcggt
     6301 cgagcgcttc gatggtcccc aacgcggtgg gaccggcgaa gagcagaaca agaaggtcgt
     6361 ccagcgcctc actgggccac ggaatcggca tctctggcgc gctggccgcc aggcgactca
     6421 gcgtgccggc gccgatcggc aatccgttgt cggccgccgc ggcggccacc cgcaacacca
     6481 gaccgacgtc ggtttcgggc gaagcatcgc gggcgagcac gatttcgttg gcgtactcga
     6541 cgaccccttc gtccagcggt cgccgcttcg gccgccgcac cagggccgac acgccgcgcc
     6601 gcggcagcgc gttctgggcc gtccgcagcc cggtcaccgc gtggtaggcg atcgtgcggg
     6661 ccgcgccgga cagcctgcgc gccaagtcga acctgtcgcc gaaattcagc gacgcgctga
     6721 tctcgtcggc gaactgggcc agcaacagat cacgtccgcg gcccgatacg cggtgcagct
     6781 cggtgcgcac gtcgagcaac gtcaggtacg cggcgtccag cgaacccgcc ggcacgttcg
     6841 ggttggacat gccgtggcgg tcgatgagct gggcgaccgc cagcgcgtcg agcagttgga
     6901 cgtcgcgaag gccgccgcga cccgacttca ggtctggctc ggcccgttgc gagatccggc
     6961 cgcaacgcag ccaacgggca tgcgtcatct cgaccagctc gtccattcgg gaacgaatcg
     7021 cggtgcgcca ctgccgtcgc gccccgccga tcagctccgt cgacagccgc gcttcgccgg
     7081 cgatatggcg cgcgtccaac atgcccaggg ccaccaccat gtcggtgttg gcgacgctca
     7141 gcgcgccgga caccgtccgc acgctgtggt cgagccgaac gttggcgtcc cacagcggat
     7201 accacagccg atccgcgacc cgctccagca catcggcagg tttgttgtcg tgcaccagca
     7261 tcaggtccag atccgagtac ggcagcagct cgcgacggcc caggccgccg atcccgacta
     7321 tcgcgaagcc gctgtcgtcg gtgatcccga tctctgccgc cctggcggtc agccacgact
     7381 cgtgcagctc cagccaggct tggcgcagcc cggccgcatc cagttcaaca gcctcagaca
     7441 gcagttggcg ccgcgaggcg gctaaatcgc tcgccccgct ggcgcttcct gtccccgttt
     7501 tctccagcga tcccgatgtc ccggacgggt cggacgggtg gggtttcata ccctcccggc
     7561 cctcccggcc cgtccgggtc ggatcagcaa tctgcggcta gcgtcacaag gcatcggttc
     7621 cgcgttcacc ggtacgcacg cgcacgatgg tttccacggg gctgacccag accttgccgt
     7681 caccgatctt gccggtacgc gccgaccgaa cgatgctgtc cacgaccttg tcgacgatgg
     7741 agtcgtcgac gacaacctcg atccgcacct ttggcacgaa atcgaccgag tactcggcac
     7801 cgcggtaaac ctcggtgtgg cccttctgcc gtccatagcc ctggacctcg ctgaccgtca
     7861 tccccagcac gccggcgtcc tccaggctgg ttttgacgtc gtcgagggtg aacggcttaa
     7921 cgatcgcggt gatcagcttc atttgtgctc cgcctccaat ttggcgccca ctatgtcctc
     7981 ctggccgtta cgagtttcct ccacgagcgc acgaggagga agcaccgaac cggtgaccag
     8041 agcgaaatcg tatccactct cggcgtgctc ggcctcgtcg atgcccgagc tctcttcctc
     8101 cgcaccgagc cgcagcccga tggtgtactt caggatcaaa gcgatgatca gcgtgacggt
     8161 ggccgagtag aagaggacgc tgaacgcgcc gacggcttgc cgttccagct gcgaccaacc
     8221 gccgccgtag aacaggccct tcgatacccc ggtgacgccg ttgatggccg ggctctccgg
     8281 agcggcgagc agaccgacca tcagcgtgcc ggccaggcca ccgatcaggt gcactccgac
     8341 cacgtcgagc gagtcgtcga agccgagctt gaacttcagg ccgcacgcca ctgcgcacaa
     8401 tgcaccggca acaaagccga tcaccagggc acccagcacg ttgaccgacg agcaggacgg
     8461 tgtgatggcg accagtccgg ccacgatgcc cgaggccgca cccagcgtgg tggctttgcc
     8521 gtcgcggatg cgctcggtga gcatccagct cagcatcgcc gcgcccgtcg cgacagtggt
     8581 ggtcatgaac gtcgacccgg cgacgccgtt cgcgctggtc gccgatccgg cgttgaagcc
     8641 gtaccagccg aaccacagca atccggcacc gagcatgacg aacggcaggt tgtgcgggcg
     8701 gaacagtgtg gtgggccagc cgcggcgctt gcccaggacg atcgccagca ccaggccggc
     8761 cacacctgag ttgatatgga cggcggtgcc gccggcgaag tcgatcgcgt gcagtttgtt
     8821 ggcgatccag ccgccgtgct cggaggcgaa tccgtcaaac gcgaagaccc agtgcgcgac
     8881 cgggaagtag acgaatgtcg cccacagtcc ggcgaacacc agccaggcac tgaacttcag
     8941 ccgatcggac acggcacccg agatcagggc gaccgtgatg atcgcgaaca tcagctggaa
     9001 ggcgacgaac acggtggcgg gcaatgtgcc ggccaacggg atgtccaccg ccgtgacgcc
     9061 cttggtcggg tcggcggcca cggaattgac gccgatgaga cccttcaggc cccagaagtc
     9121 ggtcggcttg cccatgacgt tgccgacgtc gtcgccgaac gcgatcgagt agccgtacaa
     9181 cacccataag actgtcacca cgcccatcgc gctgatgctc atcatgagca tgttcaaaac
     9241 gctcctggcg cgcaccatgc cgccatagaa gaacgccagt cctggcgtca tcaatagcac
     9301 cagcgcggaa ctcgccagca tccaggcggt atcgcctgta ttgggctggc ccagtatcgg
     9361 gtatgtcact cgctatcacc tccggtcggc cttcaactgg ccgtcagacg tctgcctgat
     9421 gacctgaacc agaaccatgc gcaatcgttg tttcggcaat ggggccaccg tgtttcggcg
     9481 ggattaacgt cccggttgcc gtgtaaggct ttcagccgag cagggcgtct acaaaagcgg
     9541 ccggctcgaa cggcgccaga tcgtcgggac cttcgcccag cccgaccagc ttcaccggca
     9601 ccccgagttc ttgttgaacg cggaaaacta tgccgccctt ggccgttccg tccagcttgg
     9661 tcagcacggc gccggtgatc tcgacgacgt cggcgaacac gcgggcctgc gccagcccgt
     9721 tctgaccgat ggtggcgtcg agcaccagca acacctcgtc gaccgccgca cgccgggtta
     9781 ccacccgctt gaccttgtcg agctcgtcca tcagcccaac cttggtgtgc agccgccccg
     9841 cggtgtcgat gaccaccacg tcggcgccgg acgcgatgcc cttgtcgacg gcgtcgaacg
     9901 ccaccgacgc cgggtcggcg ccttcgggtc cgcgcaccac ctccgcaccc acgcgggcgg
     9961 cccaggtctg caattggtcg gcggcggcgg cccggaaggt gtccgccgcg cccagcacga
    10021 cgcgccgccc gtcggcgacc agaacccgcg ccagcttgcc gacggtggtg gttttcccgg
    10081 tgccgttgac gccgacgacc agcagcaccg acggatggtc ggcgtgcggg agcgcgcgga
    10141 tcgagcgatc catgccgggt tgcagctctt tgatcagcac atcgcggagc acggccttag
    10201 cgtcggcttc agttcgcaca ttgccgctgg ccagccgggc gcgcagctcc agaatcaccg
    10261 acgccgtggc gaccgggccg aggtcggcga tcagcagggt gtcctcgacg tcttgccagg
    10321 agtcttcgtc caggtcgccg ccaccgatca ggcccagcat gctgcgcccg agcgcattct
    10381 gcgacctggc cagccggccg cgcagccgct ccagccggcc ttcgggaggg gcgatggcgt
    10441 cgatctcggc ggtctcgggg gcgtccggga cttccggggc ctcgggggcc ggcggggctt
    10501 ccgggactgc cggggccggc ggggctgccg gcggggcttg cggtgtcggg gctgcgggtg
    10561 gcggcgcgac gacctcgggt tccgggagtt gaacgtcgga aatcgtgcgt cgcggcgcgt
    10621 cgcgcggaac ggtcgcgtcg tcgccgaccg cgggcagccc gctggtgtcg atccggtctt
    10681 cgacggttgg cgtgcggctg aacatgatgc cggaggatgc ggtgtaaccg ccggagcggt
    10741 cgatggcgct gggctggggc ccttgcgagg gaggggtcag gcggatccgt cgccggcggt
    10801 agcgcaccag gcccaggacc agcgcggcga tgacgaccag ggcggcgacg accgcaatcg
    10861 cgatccaaag accttgtgac acgctgacat tcttccaagc ggtccggcgc cggcgggacc
    10921 ggccctactt cggcggccgc cgcttgctgt gccgaagagc ctcaggagga gctggcgacc
    10981 agccgcccga cttgctgccc gcgcatgcgc tgcgagatca ccgcggtgat gccgtcgccc
    11041 tgcatggtca cgccatacag cgcgtcggcg acctccatcg tcggcttctg gtgggtgatg
    11101 atgatgatct gcgaccggtc tcgcagctgc tcgaacaggc cgatcagccg gcgcaggttg
    11161 gtgtcgtcga gcgccgcctc cacctcgtcc atgatgtaga acggcgacgg gcgggcccgg
    11221 aagatcgcca ccagcatcgc caccgccgtc agcgccttct cgccaccgga cagcaacgac
    11281 agcctggtga tcttcttgcc gggcgggcgg gcctccacct cgatgccggt ggccagcatg
    11341 tcgctcggat cggtcagccg cagccgcccc tccccgcccg ggaacaacga ggcgaacacg
    11401 tccctgaact cgcgttccac gtcggcgaag gcgtcgctga acacctgcag gatgcggtcg
    11461 tcgacgtcgg cgacgacgcc gagcaggtcc ttgcgggcgc ccttgacgtc ctccagctgg
    11521 gtggacagga agttgtagcg ctcctcgagg gcagcgaact cctcgagcgc cagcggattg
    11581 accctgccca gttcggccag ctcgcgctcg gcccgcttgg cccggcgctc ctgggtgacc
    11641 cggtcatagg gcatcggcgc gggcgcggtc acctgctcgc cccgctcccg ggcctgctcg
    11701 aattcggcca cctcgagctc ggtcggtggc agcggaacct gcgggccgta ctcggcgacc
    11761 aggtcggccg gcgccatgcc gaattgctcg agcaccatct gctcgagttg ctcgatgcgc
    11821 agcgccgcct gcgcgttggc cacctcgtcg cggtgcagtg aatcggtcag ggtggccacc
    11881 cgggtgccca gcgcgtgcac ctcgtcgcgc accgcagtca tcgccgtcga tcgcccctgg
    11941 cgttcggcgg acagggcgtc gcgaattcgg gatgtcgcgt caaccacccg gtccaatcgc
    12001 ccggccagga gccgcccggc gtcggccacc gccgcggcca cggcggccgc gcgccgccgc
    12061 gccgcgcgcg cttgctgggc ccgccaccgc gcttcg
//
LOCUS       DASSGQ010000333         2867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_723025,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000333 DASSGQ010000000
VERSION     DASSGQ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723025"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1368)
                     /locus_tag="VFI55_14540"
     CDS             complement(124..1368)
                     /locus_tag="VFI55_14540"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET7075284.1"
                     /translation="MPAATAAAQWAQIQAGAPQVVVTMQRYLQRLAAFQAPASVDVAE
                     NSLRIFAAWMLGAGLDCVAAVRRDDIEDFKVWLANKPRPRGGTITPETHRQRLRMVRS
                     FFERIIEWDWSDAPARNPVINGDIPKKPEPLPRFLDDAQAARFMAAARASTEPRDRLV
                     VELLARTGMRVGELVNLDADAVVQIGGNHWLRIPLGKLRNDRYVPLHPQLVRLLSDWT
                     AANLEHIRAHRRLVVDHRGPVNRHSISRIIHRIGHAAGVEGVHPHRLRHTLATQAINR
                     GMRLEAIAALLGHKKMEMTLIYARIANRVVADEYAAVSAKIDALYGQQPEIPADYETT
                     GMARLRRETHARMLGNGLYTRPVELDCRMESACETCAYFRTSIQFLPTLTRQRDHARE
                     RGQANRAELFDKIIGGIDDTPA"
     gene            complement(1408..2469)
                     /locus_tag="VFI55_14545"
     CDS             complement(1408..2469)
                     /locus_tag="VFI55_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005564334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET7075285.1"
                     /translation="MTDFPCLVRWAAASGQTRYRLGDPLVDRYLEFVAGRARPNTLRA
                     VAFDLETFFAVVGKDPRAVTAADVFDFLAHQRGDRSVVRLIDRESGLSARTIARRLSS
                     VSGLYAYAIARGDTGVTRNPVPRGLSTRREGGTRKSRLAPLVRVPRTLPTILSPGEAD
                     RLMAALRTHRDRAMVAAMLFGGLRRCEVLGLRLVDIQVADRRVFIVEGKGGHHRVVPI
                     ANRFFDALGDYLHDERPSVGNTEWVFVVLKGSRRGQPLSSEGLDEVLDGARRRAGLQR
                     ATCHQLRHTCLTRLREAGMALEAVQAQAGHLSIESTRVYLHLTNDWLAAEYRRAASLI
                     DADTAAVEAMLAVQEAAAP"
BASE COUNT          479 a          970 c          953 g          465 t
ORIGIN      
        1 atcggcatgt ccgtccatgt cggcgcacgg tctgccgtgt tgacagcgcg cataacagcg
       61 accgcggacc tgatcacacc aacaaggggc gggcacgtaa tcggcgttat acgggtgatc
      121 gtgtcaagcg ggtgtgtcat cgatccctcc gatgatcttg tcgaagagtt cggctcggtt
      181 ggcttgtcct cgttcgcggg cgtggtcgcg ttgccgggtc agggtgggta ggaactggat
      241 gctggtgcgg aagtaggcgc aggtttcgca ggctgactcc atccggcagt cgagttcgac
      301 cggtcgggtg tacaggccgt tgccgagcat gcgggcgtgt gtttcgcggc gtagccgggc
      361 catgccggtg gtttcgtagt cggcggggat ctcgggctgc tggccgtaga gggcgtcgat
      421 cttggcgctg acggcggcgt attcgtcggc gacgacgcgg ttggcgatgc gcgcgtagat
      481 gagcgtcatc tccatcttct tgtggccgag cagtgcggcg atcgcctcta gtcgcatgcc
      541 gcggttgatc gcctgggtcg caagagtgtg ccggagccgg tgtgggtgca ctccttcgac
      601 gccggcggcg tgtccgatgc ggtggatgat tctgctgatc gagtgccggt tgaccggacc
      661 gcgatggtcg acgacgaggc ggcggtgcgc gcggatgtgc tcgaggttcg ccgcggtcca
      721 gtcgctgagc agtctcacca gttgcgggtg cagcgggacg tagcggtcgt tgcgcagctt
      781 gcccagcggg atgcgtagcc agtggttgcc gccgatctgc acgacagcgt ccgcgtcgag
      841 gttgacgagc tcgccaacgc gcatgccggt gcgggccagc aattcgacaa cgagccgatc
      901 ccgcggctcg gtgctggcac gagcagcggc catgaacctg gctgcctggg cgtcgtcgag
      961 gaagcgcggc agcggttccg gcttcttcgg gatgtcgccg ttgatgaccg ggttgcgggc
     1021 cggcgcgtcg gaccagtccc actcgatgat ccgctcgaag aacgagcgca ccatccgcag
     1081 tcgctgccgg tgcgtctccg gcgtgatcgt cccgccgcgt ggacgcggct tgttggccag
     1141 ccagaccttg aagtcctcga tgtcatcgcg gcgcaccgca gcgacacagt cgaggccagc
     1201 gccgagcatc catgcagcga agatgcgcag cgagttctcc gcgacgtcga cgctggccgg
     1261 cgcttgaaag gccgccagcc gctgcagata ccgctgcatc gtcaccacga cctgcggggc
     1321 gcctgcttgg atctgcgccc actgcgcagc tgccgtggct gccggcaacc gcgcgatcag
     1381 gcaccggcgc ggcagcaggg tgcgcgctca cggcgcagcc gcctcttgaa cggcgagcat
     1441 cgcctcgacc gctgcagtgt cggcgtcaat caacgacgcg gcacgacggt attcggccgc
     1501 gagccagtca ttggtcagat gcagatagac gcgcgtcgac tcgatcgaca ggtgaccggc
     1561 ctgggcctgg accgcctcca acgccatccc ggcctcacgc agccgcgtca gacaggtgtg
     1621 acgcaactga tgacaggtcg cccgctgcag gcccgcgcgg cggcgtgccc cgtccaggac
     1681 ctcgtcgagc ccctcgctcg acagcggctg cccgcgacgc gaccccttca gcacgacaaa
     1741 cacccactcg gtgttcccca cgctcggccg ctcgtcgtgc aggtagtcgc cgagcgcgtc
     1801 gaagaaccgg ttcgcgatcg gcaccacccg gtgatgcccg cccttgccct cgacgatgaa
     1861 cacccgtcga tccgccacct ggatgtcgac caggcgcagt ccgaggacct cgcagcggcg
     1921 cagcccgcca aacagcatcg ccgccaccat cgcccggtcc cggtgcgtgc gcagcgccgc
     1981 catcagtcga tcggcctcac ccggcgacaa aatcgtcggc aaagtgcgcg gcacccgcac
     2041 caacggcgcc agccgcgact tgcgcgtgcc gccctcacgc cgagtcgaca gcccacgcgg
     2101 caccgggttc ctcgtcaccc cggtgtcccc gcgagcgatc gcataggcat acaaccccga
     2161 caccgatgac agccggcgag cgattgtgcg cgccgacaaa cccgactcac gatcgatgag
     2221 ccgaaccaca ctgcgatcac cccgctggtg ggcgaggaag tcgaacacat ccgccgccgt
     2281 cacagcccgc ggatccttcc cgaccaccgc gaagaacgtc tccagatcga acgccaccgc
     2341 ccgcagcgtg ttcggccgag cccggccagc cacgaactcc aagtaccggt ccaccagcgg
     2401 atcaccgagt cgatatcgcg tctggcccga cgcagcagcc cagcgcacca gacaaggaaa
     2461 gtcagtcatg atcgaacgct gacagccgac tcgcagccgt ccggtacgac acgaacatca
     2521 cccgtgtatt aggcgatctg cgccaggtct tgaacatgaa cggccctggc ctcggttacg
     2581 atgcgtgatc gtccgaatgg gcttccggca gggggcgttt gcccgcgccc gagatggaag
     2641 tcgtgcagcg ccttcgcgac gatcacatgg cctccgacct ggcgtatgac acgttcatcc
     2701 agctcggcga cccgggctgt tgcactcaag cctgaccgct ggagccggat gggagcgccg
     2761 ccagcccgtc ggcggtggag gtcctcctgg gtatagccgg tgtgactaac tgcgtgacaa
     2821 tgaccgcgat cgaaaggaga cttccgtaga cctctgcaga cagaaga
//
LOCUS       DASSGQ010000334         7936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_54828, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000334 DASSGQ010000000
VERSION     DASSGQ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54828"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VFI55_14550"
     CDS             complement(<1..465)
                     /locus_tag="VFI55_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7075286.1"
                     /translation="MSDAIHVPSEQSSPASALGLQQRQVAWERLGTEQFDVVVIGGGV
                     VGCGCALDAATRGLKVALVEARDFASGTSSRSSKMFHGGLRYLEQLEFGLVREALYER
                     ELSLNTLAPHLVKPMPFLFPLTKRGWERPYVAAGIFLYDRLGGAKSVPPQKHL"
     gene            complement(541..1992)
                     /locus_tag="VFI55_14555"
                     /pseudo
     CDS             complement(541..1992)
                     /locus_tag="VFI55_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217820.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone dehydrogenase"
     gene            2177..2656
                     /locus_tag="VFI55_14560"
     CDS             2177..2656
                     /locus_tag="VFI55_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HET7075287.1"
                     /translation="MPLYAAYGSNMHPEQMQMRAPHSPMAGTGWLHGWRLTFGGEDIG
                     WEGALATIVEDPDSKVFVVLYDMTSADEMNLDRWEGSEFGVHKKIRCRVERLSSDTTT
                     DPALAWLYVLDAWEGGLPSARYLGVMADAAEIAGAPEDYVHDLRTRPASNIGPGTSA"
     gene            complement(2730..3611)
                     /locus_tag="VFI55_14565"
     CDS             complement(2730..3611)
                     /locus_tag="VFI55_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075288.1"
                     /translation="MNAALGQLLDAQGGVVTSAQVLKFLTRRGLEAHVNRGALQKIWH
                     GIYGRGEVTTAMRLHGLDLVAGATVAVCMGTAAAAYGFDTERTVDLHVLNPDGRQLRS
                     VDGLVVHRRAGAPLTLVGGRPATTPAWTAIEIARGVRRPRALATLDAALRSGTCRRVD
                     LEAAGELQSGRRGIVAARELLGLANPLAESPMESETRLVMIDAGLPQPLLQYEIVDAR
                     GRLWRLDFAWPNCRVAAEYDGVDWHSGPEAFLRDRRRSSALQELGWLIVPIVAEDVRH
                     RPGELIRRLEAHLRRAA"
     gene            complement(3718..4887)
                     /locus_tag="VFI55_14570"
     CDS             complement(3718..4887)
                     /locus_tag="VFI55_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET7075289.1"
                     /translation="MSLRDAAESWLAKHHDDLIAWRRHIHRYPELGRQEYATTQFVAE
                     RLADAGLNPKVLPGGTGLVCDMGPEREPRIALRADMDALPMAERTGAPYASTMPNLAH
                     ACGHDAHTAILLGTALALASAPELPVGVRLIFQAAEELMPGGAIDAIAAGAISGVSRI
                     FALHCDPRLDVGKIAVRHGPITSAADQIEITLYSPGGHTSRPHLTADLVYGLGTLITG
                     LPGVLSRRIDPRNSTVLVWGAVNAGVAPNAIPQTGVLAGTVRTASRHTWVDLEELIRE
                     SVAGLLSPLAIEHTLQYRRGVPPVVNDDVSTRIFTHAIEAVGPDALADTRQSGGGEDF
                     SWYLEEIPGAMARLGVWSGEGPQLDLHQPNFDLDERALAVGLRVMVNIIEQSALV"
     gene            complement(4884..6077)
                     /locus_tag="VFI55_14575"
     CDS             complement(4884..6077)
                     /locus_tag="VFI55_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET7075290.1"
                     /translation="MPTTTALDSVEDVVRRRGADLVELSHAIHAEPELAFAEHRSAAK
                     AQALVAERGFEITAPAGGLDTAFRADFGSGPLVIGVCAEYDALPEIGHACGHNIIAAS
                     AVGTALALAEVADDLGLRVALLGTPAEEAGGGKALLLRAGIFDDIAAAVMVHPGPMDI
                     AAARSLALSEVIVNYRGKESHAAVAPYLGVNAADAVTVAQVAIGLLRQQLAPGQLVHG
                     IVTNGGQAVNVIPGHATLQYAMRAIESDSLQQLEGRMFACFAAGALATGCEYEIDNPA
                     PAYEELKPDPWLADICREEMRRLGREPVAPEFEAGLPMGSTDMGNVTQVLPGIHPVIG
                     VDAGGATVHQRAFAAAAAGPSADRAVVDGAIMLARTVVHLAQTPGERDRVLAAQQRRN
                     VEAHR"
     gene            6142..6948
                     /locus_tag="VFI55_14580"
     CDS             6142..6948
                     /locus_tag="VFI55_14580"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET7075291.1"
                     /translation="MSDRVPDPDELARRAAHVIRERTGIDEYDVAIVLGSGWSPAVAA
                     LGSPTVVVPQADIPGFAPPSAVGHTGELLAMRIGENRVLVLVGRVHAYEGHDLRHVVH
                     PVRAACAAGARIVVLTNAAGGLRPDMAIGQPVLISDHLNLTARSPLVGAQFVDLTDAY
                     APRLRELARQAAPELTEGVYAGLPGPHYETPAEVRMLRTLGADMVGMSTVHETIAARA
                     AGAEVLGVSLVTNLAAGIAGHPLSHAEVLAAGAASATRMGALLADVIARF"
     gene            6952..>7936
                     /locus_tag="VFI55_14585"
     CDS             6952..>7936
                     /locus_tag="VFI55_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HET7075292.1"
                     /translation="MTPEEWIAHDPDPATAAELAACGPDERAARFARPLRFGTAGLRG
                     PVRAGPDAMNVAVVSRATWGVARVLRDRGLAAAPVIVGRDARHGSAVFATVTAEVLAA
                     EGFSVLLWPGPVPTPVVAFAVRHTGAAAGIQITASHNPPGDNGYKVYFEGGAQIVSPT
                     DRQIETAIVAAPPADQITRAPVEPADTALVERYIERAATVRRSTGSVRVVLTPMHGVG
                     GAVAVETLLRAGFTTVHTVATQFAPDPDFPSVAFPNPEEPGATDALLALAEDVRADVA
                     IALDPDADRCAVGIPAGIAGASGWRMLSGDETGWLLGDYILSRTASPHTAVV"
BASE COUNT         1244 a         2771 c         2709 g         1212 t
ORIGIN      
        1 caaatgcttt tgcggcggaa cggatttcgc gccaccgaga cggtcgtaga ggaagatgcc
       61 ggcggcgacg tatgggcgtt cccacccgcg cttggtcagc gggaacagaa acggcatcgg
      121 cttgaccaga tgcggcgcca gcgtgttcag cgacagttca cgctcgtaga gcgcctcgcg
      181 gaccagcccg aattcgagtt gctcgaggta gcgcagcccg ccgtggaaca tcttcgacga
      241 gcggctcgac gtgcccgagg cgaaatcgcg cgcctcgacc agcgccacct tgagcccgcg
      301 cgtggcggca tccaaggcgc agccgcagcc cacgacgccg ccgccgatca ccaccacgtc
      361 gaactgctcg gtgccgagcc gctcccaggc cacctggcgt tgctgcagtc ccagcgcgga
      421 ggccggcgaa ctttgctcgc tgggtacgtg gatcgcgtcg ctcacggcag aggactcctc
      481 acttggggaa actcctgtcg gttactcgtc agtaggactg tgctccacca aggctagtct
      541 ttaccggatc atgccagcgt ccggcgatcg cgccgttcag tccagatcgt cgtgggccat
      601 cagccggcgc gccgcctcgg tgatcgaacc ggacaacgac gggtagacgg ccaacgtgtc
      661 ggccaactcg ttgacggtga tgcggttttg cacggccatg gcgatcggca ggatcagctc
      721 ggaggcgatc ggagccacca ccacgccgcc gatcaccacg ccggtagagc gccggcagaa
      781 gatcttgacg aaaccttgcc ggagccccga catcttggct cgggcgttgg tccgcaacgg
      841 cagcatgacc gtgcgggcca gcaccgagcc gtcgtcgatc atcgactgcg gcacaccgac
      901 cgcggcgatc tcgggcctgg tgaacaccgt tgccgcgacc gtgcgcagcc ggatcgggct
      961 gacgccctcg cccagcgcgt ggtacatcgc gatccggccc tgcatcgccg cgaccgacgc
     1021 caacggaagc aggccggtac agtcgcccgc ggcgtagatg ccaggcaccg acgtccgcga
     1081 cacgcggtcc acggtcaggt aattgccccg tcccaactcg atgccgaccc gttccaggcc
     1141 gaggccgccg gtgttgggga ccgacccgat cgtcatcagg gcgtggctgc cctcgacggt
     1201 gcggccgtcg gtcatcgtca ccaggacgcc ggtgtcggtc cgggtgacgg attgcgcacg
     1261 ggcgttcttg aacagccgga ccccgcgctc ggagaacgac tcctccagga ccagcgcggc
     1321 gtcggcatcc tcgtagggca ggacgtggtc ctggctggcc accaccgtca cctgcacgcc
     1381 cagctcggtg taggcgttga cgaactcggc gccggtgacc cccgagccga ccacgatgag
     1441 gtggtcgggt agtgcggtca ggtcgtagag ctgccgccaa gtcaggatgc gttcgccgtc
     1501 gggctgtgcc gacggcagga tccgcgggct ggccccggtg gcgatcagca cgacatcggc
     1561 gtcgtgctca gtgatggtgc cgtcggaggc cgtcgcgcgg acgcggtgat gggccatgcc
     1621 cggcgtgggg tcgaccagct cgccgcggcc ggcaatcacc tcgacgccca cgctgagcag
     1681 ctgagcggtg atatcggccg actgctccgt ggccagcgat ttgacccggg catggatttg
     1741 cggcagcgag atcttggcat cgtcgatgtc gatgtcgaag cccagccgcg gcgcccggcg
     1801 aagttcggtg cgcagccagg tggacgcgat gaacgtcttg gacggcacac agtcgtccag
     1861 cacggccgcc ccgccgatgc cctcggagtc gatcacgcag acttgggttg tttctgggtg
     1921 cgaggtagcg gcgacaagcg cggcctcgta accggccggc cctccgccaa gtatcacgat
     1981 gcgggtcacc acaaggcata acctagcttg gcgacgactc gcggcgaagc gcggcgcggg
     2041 gaggagccga gcaattcggg ctgagcttgg cgacgactcg cggcgaagcg cggcgcgagg
     2101 tgtgcgcgat gtgttgggga agccaactag cgctcgggtg aacaccgctt gagggtgtgc
     2161 cgtttaagct tttcccgtgc cgctctacgc cgcctacggg tcgaacatgc atcccgagca
     2221 gatgcagatg cgcgcaccgc actcgccgat ggccggaacg ggctggctgc acgggtggag
     2281 gctgacgttc ggcggcgagg acatcggctg ggaaggggcg ctggccacga tcgtcgagga
     2341 tccggattcg aaggtcttcg tcgtgcttta cgacatgact tcggcggacg agatgaacct
     2401 tgaccgctgg gagggctccg agttcggtgt ccacaagaag atccgctgcc gggtcgagcg
     2461 cctgtcctcc gacacgacga cggatcccgc gctggcctgg ctgtacgtgc tggacgcctg
     2521 ggagggcggc ctaccgtcgg cgcgctacct cggcgtgatg gccgacgccg ccgaaatcgc
     2581 gggcgcaccg gaagattacg tgcacgacct gcggacacgt ccggccagca acatcggccc
     2641 ggggaccagc gcctaattgc cggtgagcgt gcgtgtctgc acaccgacac gccggaattg
     2701 ctggcatttt gcgcaccctc gcgccagcgt tacgcggctc ttcgtagatg cgcctcaagc
     2761 cggcggataa gttcgccggg ccgatgtctg acatcctcag caacgatcgg cacgatcaac
     2821 catcccaact cttggagtgc ggacgagcgg cgacgatccc gtagaaatgc ctcgggcccg
     2881 ctatgccaat cgacgccgtc gtactcggca gcgacgcggc agttgggcca ggcaaagtcg
     2941 aggcgccaca ggcgtccccg cgcgtccacg atctcgtatt gcaatagcgg ctgcggcaat
     3001 ccggcgtcga tcatgactag gcgggtttcg ctttccatcg gcgactccgc cagcgggttt
     3061 gccaaaccca gcaactcgcg cgcggcgaca atcccccgac ggccggactg caactcaccc
     3121 gccgcctcca gatcgacgcg gcgacacgtg ccactgcgaa gggcggcgtc caacgtggcc
     3181 agcgcgcgag gccggcgcac gccgcgcgcg atctcgatcg ccgtccacgc gggcgtggtg
     3241 gccggccggc ccccgaccag ggtcagcggc gcacccgcac gccgatgcac gaccaggccg
     3301 tcgaccgatc gcaattgccg accgtcggga ttgagcacgt gcaggtccac agttcgctcg
     3361 gtatcgaatc cgtaggcagc cgccgcggtg cccatacaaa ccgcgaccgt cgcgccggcc
     3421 accaggtcga gcccgtgcaa tcgcattgcg gtcgtcacct cgccgcggcc gtatatgccg
     3481 tgccaaatct tctgcagtgc accgcgattg acgtgtgctt ccaagccgcg tcgagtgagg
     3541 aacttcagca cttgggcgga tgtcaccact ccgccttggg catcaagcaa ctggccgagc
     3601 gccgcgttca cgcccgttat cgtcggcgcg cgatggatcg tcgacaattc acctgccgag
     3661 ggtgcgcaga atgccgggaa acacggcgtg tcgctgtacc gacacgcacg ctcgcagtca
     3721 gacgagtgcg gactgctcga tgatgttgac catcacgcgc aggccgaccg ccagggcccg
     3781 ctcgtcgaga tcgaagttcg gctggtgcag gtccagctgt gggccctcgc ccgaccacac
     3841 gcccaatcgc gccatcgcgc cggggatctc ctccagatac caggagaagt cctcaccgcc
     3901 accggactgc cgcgtgtcag ccagcgcatc ggggccgacg gcctcgatcg cgtgcgtgaa
     3961 gatgcgtgtc gacacgtcgt cgttgaccac cggcggcact ccgcgccggt attgcagggt
     4021 gtgttcgatc gccagcggcg acagcaggcc cgcgacggac tcgcggatga gctcctcaag
     4081 gtcgacccag gtatgccggc tggcggtgcg aacggtgccg gccaggacgc cggtctgcgg
     4141 gatcgcgttg ggagccacgc ccgcattgac cgcgccccac accaacaccg tgctgttgcg
     4201 tgggtcgatg cggcgcgaca gcacccccgg cagccccgtg atcagcgtgc ccagcccgta
     4261 aaccaagtcg gcggtcagat gtggccgcga cgtgtgtcca cccggcgaat acagcgtgat
     4321 ctcgatctgg tcggctgccg aggtgatggg gccgtgccgg accgcgatct tgccgacgtc
     4381 gagccgggga tcgcaatgca gcgcgaagat ccgcgacacc ccgcttatcg cgccggcggc
     4441 gatcgcgtcg atcgccccac cgggcatcag ctcctcggcg gcctggaaga tcaaccgcac
     4501 cccgaccggc agctcgggcg cggacgccaa ggccagcgcc gtacccagca ggatcgcggt
     4561 gtgcgcgtca tgcccgcagg catgcgcgag gttcggcatg gtcgacgcgt acggggcgcc
     4621 ggttcgctcg gccatcggca gcgcgtccat gtcggcccgc agcgcgatcc tcggctcgcg
     4681 ctcgggaccc atatcgcaga ccagcccggt cccgccggga agcaccttcg ggttaagtcc
     4741 cgcgtcggcc aaccgctcgg cgacgaactg ggtggtggcg tactcctgcc ggcccagctc
     4801 gggatagcgg tggatgtgtc ggcgccaggc gatcaggtcg tcgtgatgct tggccaacca
     4861 cgattcggcg gcatccctga ggctcatcgg tgtgcctcga cattcctgcg ctgctgcgca
     4921 gccaagaccc gatcgcgttc gcccggcgtc tgcgccagat ggacaaccgt gcgcgccagc
     4981 atgatcgcgc cgtcgacgac cgcacggtct gcgctgggtc cggcggcggc cgccgcgaag
     5041 gcgcgctggt gcaccgtcgc accgccggcg tcgaccccga tcaccggatg gatccccggc
     5101 agcacctgcg tcacattgcc catgtcggtg ctgcccatgg gaagccccgc ctcgaactcg
     5161 ggcgccaccg gctcgcgtcc gagtcggcgc atctcctccc ggcagatatc ggcgagccat
     5221 gggtcgggtt ttagctcctc gtaggcaggc gcagggttgt cgatttcgta ttcgcagccg
     5281 gtggccagcg cacccgcggc aaagcacgcg aacatcctgc cctccagctg ctgtagcgaa
     5341 tccgattcga tcgcccgcat cgcatactgc agcgtcgcgt gccctgggat gacgttgacg
     5401 gcctgtccgc cgttggtgac gatcccgtgt accagttgcc ccggcgccag ctgctgtcgc
     5461 agcaacccta tggccacctg cgcgacggtc acagcgtcgg cggcattgac acccagatac
     5521 ggcgcgacgg ccgcatgcga ttccttgccc cggtaattca cgatcacttc ggacagcgcc
     5581 agcgagcggg ccgcggcgat gtccatcggc ccgggatgca ccatcaccgc cgctgcgatg
     5641 tcgtcgaata tccctgcccg cagcagcagc gctttgccgc cgcccgcctc ctcggcggga
     5701 gtgcccaaca gcgccacccg caagcccagg tcgtccgcca cctctgccag tgccagcgcg
     5761 gtgcccaccg ccgacgccgc gatgatgttg tggccgcacg cgtgcccgat ctcgggcagc
     5821 gcgtcgtact cggcgcacac cccgatgacc agcggtccac tgccgaagtc ggcgcggaag
     5881 gcggtgtcca aaccgccggc aggcgcggtg atctcgaaac cgcgttcggc gaccagcgcc
     5941 tgcgccttgg ccgcgctgcg atgctcggcg aatgccagct ctggctcggc atggatagcg
     6001 tgggacaact cgacaaggtc ggcaccacgt cgccgcacga catcctcgac gctgtccaat
     6061 gcggtggtag tgggcatcct cgcagtatct caccgcagtg cgcccagtca tcgccgagcg
     6121 gcttagttaa gctcgcccac tgtgagcgac cgcgtgcccg atcccgacga actcgcgcgg
     6181 cgggcggcac acgtcatccg cgagcggacc ggcatcgacg agtacgacgt cgccatcgtg
     6241 ctggggtcgg gatggtcgcc ggcggttgcg gctctgggct cccccaccgt cgtggtgccg
     6301 caggccgaca ttcccgggtt cgcaccgccg agcgccgtcg ggcataccgg tgagctgctg
     6361 gcgatgcgaa tcggcgagaa ccgggtgctg gtgttggtcg gccgggtgca cgcctacgag
     6421 ggccatgacc tgcgccacgt ggtgcacccg gtacgggcgg cctgcgcggc gggggcacgc
     6481 atcgtcgtgc tcaccaacgc ggccggcgga ctgcgcccgg acatggcgat cggccagccg
     6541 gtgctgatca gtgatcacct caacctgacc gcgcggtcac cgttggtggg cgcgcagttc
     6601 gtcgacctca ccgatgccta cgcaccgcgg ctgcgagagc tcgcccgcca ggccgccccc
     6661 gagctgacgg aaggcgtcta cgcgggcctg cccggaccgc actacgaaac gcccgcagag
     6721 gtccgaatgc tgcggacgct gggcgccgac atggtgggca tgtcgacggt ccacgagacc
     6781 atcgcggccc gggcggccgg ggccgaggtg ctgggtgtgt cgctggtgac gaacctggcg
     6841 gcggggattg cgggacatcc gttgagtcac gccgaggtgc tcgccgccgg ggccgcgtcg
     6901 gcgacccgga tgggcgccct gctcgcagac gtcatagccc ggttctgagc cgtgacgccc
     6961 gaggagtgga ttgcccacga tcccgacccg gcgacggccg ccgagctcgc tgcatgcggc
     7021 cccgacgaac gtgcggcgcg gttcgcccgc ccactcaggt tcggcaccgc aggcttgcgc
     7081 gggccggtgc gcgccggccc ggacgcgatg aacgtcgcgg tggtgtcacg cgccacctgg
     7141 ggggtggcac gggtgcttcg ggaccgcggg ctggccgccg caccggtgat cgtcgggcgt
     7201 gacgcccgcc acggatcggc ggtgttcgcc accgtgacgg ccgaagttct cgctgccgaa
     7261 ggtttttcgg tgctgttgtg gccgggtccg gtgcccacgc cggtggtggc gttcgcggtg
     7321 cgccacaccg gcgcggccgc cgggatccag atcacggcgt cgcacaatcc gccgggcgac
     7381 aacggttaca aggtgtactt cgagggcggt gcgcagatcg tctcccccac cgaccgtcag
     7441 atcgaaaccg cgatcgtcgc cgctcccccc gctgaccaga tcacccgcgc accagtcgaa
     7501 cccgccgaca ccgcactggt ggagcgctac atcgagcgcg cggccacggt gcggcgctcg
     7561 accggttcgg tgcgggtagt cctgaccccg atgcacggcg tgggcggggc ggtggctgtg
     7621 gagacgctac tgcgggccgg cttcaccacc gtgcacaccg tggcgacgca gttcgcaccg
     7681 gaccccgact tccccagcgt cgcgttcccc aaccccgaag agcccggcgc caccgacgca
     7741 ctgctcgcgc ttgccgaaga cgtccgcgcg gacgtggcca tcgcgctgga tcccgacgcc
     7801 gacagatgcg cggtcggcat ccccgcgggg atagccgggg catcgggatg gcggatgctg
     7861 tccggcgacg aaaccggttg gctgctgggc gattacatct tgtcgcgcac cgccagtccg
     7921 cacaccgcgg tggtgg
//
LOCUS       DASSGQ010000335         2684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_693070,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000335 DASSGQ010000000
VERSION     DASSGQ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693070"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFI55_14590"
     CDS             complement(<1..641)
                     /locus_tag="VFI55_14590"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HET7075293.1"
                     /translation="MPRRNERGIALILALFLVSALSVLGASLMFLSQTETNASMNYRM
                     MSQVRYAAEAGVQQASNYLLGALPQPTAADLLGPNCNRNVSPVTCKVAGVDTPVVLTA
                     TTAPGTPAANYPVAAVQTAFSAAAQGTMAAGNSTLTYNSYARLLALQTFEGYGGTPGV
                     SWTWEITGSGGLVGYSKATVEILALVELPKVPASSYGAFGTDNTCGALNFGGNV"
     gene            complement(656..1666)
                     /locus_tag="VFI55_14595"
     CDS             complement(656..1666)
                     /locus_tag="VFI55_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075294.1"
                     /translation="MMNRLRSEAGFSLAEVLVATGLMLTVSGIVTSALLQMSNHQQTI
                     WNRTEMHSGVRGATELLQQEVGQAGRVSLPGPPALPVTLDPGAGIVGVNPCTATTTVT
                     VTSTAGMFAGEWLTTFDGDNSETFAVTAIPSATTLTACFTRNHVVDPITNSPVPVGVL
                     GGFATGILPPGGTGSSANKLKLYGDINGDGNMVYVEYYCDNGDAGTAGSFNLYRNTMP
                     FDTAAAAKPALTSSMILLSNVHSNPADAGGAARPCFQYQTVPITVGGVNYTLVLDVAV
                     TLTVWTQSIDPITKQFQTETKALLNVSPRNVGFTWQFAGLAYNNRVQLPPQSVNDLAA
                     AP"
     gene            complement(1670..2242)
                     /locus_tag="VFI55_14600"
     CDS             complement(1670..2242)
                     /locus_tag="VFI55_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075295.1"
                     /translation="MADAAAHRSGLVDAMRIPLRGLLRRLRDEDGTTLIETSIAVGIL
                     FVTLAGLMSMGTIATMHTENQGHLMPRTTEYAQDKMEQLMALAYGDTTTDTVVFPAVS
                     AGGSGLAVGGSVNTAAPVNLYVDWLASDGSLLGGGTAAPGSWFYERVWQVSTPAAGTK
                     QITVAVTVRSAIGGFMLPKSTVAAIKSSVF"
     gene            complement(2197..2619)
                     /locus_tag="VFI55_14605"
     CDS             complement(2197..2619)
                     /locus_tag="VFI55_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075296.1"
                     /translation="MRAASIFGRERLYVDLAARSYRQETWDKTTSAWIADGASAYLSQ
                     NVNYSFGVVGAPPPNTQAAIAQSPACKNNAGTADIANTACIIFNSRGLPIDNAGQPFV
                     YGLYVSDGTAVYGVTVSATGTVRMWRTPPRTVPAWSMQ"
BASE COUNT          416 a          870 c          931 g          467 t
ORIGIN      
        1 acgttgccgc cgaagttcaa ggcgccgcag gtattgtcgg tgccgaacgc gccgtaggag
       61 ctcgccggga ccttcggcag ctcgaccagc gcgagaatct cgacggtcgc cttcgagtat
      121 ccgaccagac cgccgctgcc ggtgatctcc caggtccacg acacgcccgg ggtgccgccg
      181 tagccctcga acgtctgcag cgccaacagg cgggcgtacg agttgtaggt cagggtgctg
      241 ttgcccgccg ccatcgtccc ctgggcggcg gcgctgaacg cggtctgcac cgccgcgacc
      301 ggatagttcg cagctggcgt gccaggcgca gtcgtcgccg tgaggacgac gggggtgtcc
      361 accccagcga ctttgcaggt gaccggcgac acgttgcggt tgcagttcgg gcccaggaga
      421 tccgccgcgg tcggctgcgg cagcgcgccc agcaggtagt tcgaggcctg ctgcacgccg
      481 gcttcggcgg catagcgcac ctgcgacatc atgcggtagt tcatgctggc gttggtttcc
      541 gtctgcgaca ggaacatcaa cgaggcgccc agcacggaca gcgccgagac caggaacagc
      601 gcgagaatca gggcgattcc gcgttcgttg cggcggggca tctgattcag gctcctcatg
      661 gcgccgccgc gagatcgttg acgctctgcg gcggcagctg cacgcgattg ttgtaggcca
      721 gcccggcaaa ctgccaggtg aacccgacgt tgcgcggcga gacgttcagc agcgccttgg
      781 tctcggtctg gaactgcttc gtgatcggat cgatcgactg cgtccacacc gtcagcgtca
      841 ccgcgacgtc gaggaccagg gtgtagttga ccccgccgac cgtgatcggc accgtctggt
      901 actggaagca cgggcgcgcc gcgccgccgg cgtcggccgg gttcgagtgc acgttgctca
      961 gcaggatcat cgagctggtc agcgccggct tggccgcggc cgcagtgtcg aacggcatgg
     1021 tgttgcgata gagattgaac gaccctgctg tcccggcgtc gccgttgtcg cagtagtact
     1081 cgacgtagac catgttgccg tcgccgttga tgtcgccgta cagcttcagc ttgttcgcgc
     1141 tggagccggt tcctccgggc gggaggatgc cggtcgcgaa cccgccgagg acgccgactg
     1201 gaacgggtga gttcgtaatc gggtccacga cgtggttacg cgtgaaacag gccgtgagcg
     1261 tggtcgccga cgggatcgcc gtgactgcga acgtttcgga gttgtcgccg tcgaaggtcg
     1321 tcaaccactc accggcgaac atgccggccg tcgacgtcac ggtcaccgtg gtcgtggccg
     1381 tacacgggtt gaccccgacg atgcctgccc ccgggtcgag ggtcaccgga agagcgggag
     1441 gaccgggcag gctgacccgt cccgcctgtc cgacttcctg ctgcagcagt tcggtggcgc
     1501 cgcggacgcc gctgtgcatc tcggtgcgat tccaaatcgt ctgctggtga ttgctcatct
     1561 gcagcagcgc gctggtcacg atgcccgaga ccgtgagcat cagtcccgtc gccacgagca
     1621 cttcggcgag ggagaagccg gcttccgagc gcagtctgtt catcatggct tagaagaccg
     1681 acgacttgat cgccgccacc gtggacttgg gcagcatgaa gccgccgatg gctgaacgga
     1741 ccgtgacggc gaccgtgatc tgcttggtgc ccgccgccgg cgtgctgacc tgccagacgc
     1801 gttcgtagaa ccacgaccca ggggctgcgg tgcccccgcc gagcaggctg ccgtcgctcg
     1861 ccagccagtc gacgtagagg ttcaccggcg ccgccgtgtt gacgctgccg ccgaccgcga
     1921 ggccgctccc gccggcgctg acggcgggga acacgacggt gtcggtggtg gtgtcgccgt
     1981 aggcgagcgc catcagctgt tccatcttgt cctgcgcata ctcggtggtg cgcggcatca
     2041 gatgcccctg gttctccgtg tgcatcgtcg cgatcgtgcc catcgacatc agcccagcca
     2101 gcgtcacgaa caggatgccg accgcaatcg acgtctcgat cagcgttgtg ccgtcttcgt
     2161 cccgaagccg ccgaagcagt ccgcgcagcg ggatcctcat tgcatcgacc aggccggaac
     2221 ggtgcgcggc ggcgtccgcc acatgcggac ggtgccggtc gccgaaacgg tgaccccgta
     2281 gaccgcggtt ccgtcgctca cgtacagccc gtagacgaat ggctggccgg cgttgtcgat
     2341 cggcaggccg cgcgagttga agatgatgca ggcggtattg gcgatgtcgg cggtgcccgc
     2401 gttgttcttg cacgccggcg actgagcgat cgcggcttgc gtgttcggcg gcggcgcgcc
     2461 gacgacgccg aaggaatagt tcacgttctg ggacagatag gcggaagcgc cgtcggcaat
     2521 ccacgccgag gtggttttgt cccacgtttc ctggcggtag ctcctcgcgg cgaggtcgac
     2581 atagaggcgc tcgcgtccga agatcgacgc ggcgcgcatc tttccgaggc cggtggcgtt
     2641 cgccacgctt cgagcgtcgc cgctcaagcg gtagtaggcg aggt
//
LOCUS       DASSGQ010000336         7814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_359744,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000336 DASSGQ010000000
VERSION     DASSGQ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7814
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359744"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VFI55_14610"
     CDS             <1..635
                     /locus_tag="VFI55_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HET7075297.1"
                     /translation="NPRTAATYAARIVGLAAMLGATVGSMVALAGTVDAAPGMHGDPA
                     AAARYWRYQRQDLDCGEMAVADVVGQITGQEPTEDEVTGAAESIPSVSHPGPIYKPSG
                     KTKNLDLPVLLAHYGIPAGAVHTNIDAIGQDLDQGHKVIVGVNDKTIWNEPGDRTQQN
                     HFVVVTGIDTNANVVHLNDSGIKAGRDEQVSIATFETAWATDDNFAVVTK"
     gene            complement(744..1355)
                     /gene="recR"
                     /locus_tag="VFI55_14615"
     CDS             complement(744..1355)
                     /gene="recR"
                     /locus_tag="VFI55_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HET7075298.1"
                     /translation="MFEGPVQDLIDELGKLPGIGPKSAQRIAFHLLSVEPPEIDRLTA
                     VLGKVRDGVRFCAVCGNVSDDERCRICADPRRDGSQVCVVEEAKDIQAVERTREFRGR
                     YHVLGGALDPLSGVGPDQLRIRELLSRIGERVDDVDITEVIIATDPNTEGEATATYLV
                     RMLRDIPGLTVTRIASGLPMGGDLEFADELTLGRALTGRRAMV"
     gene            complement(1357..1701)
                     /locus_tag="VFI55_14620"
     CDS             complement(1357..1701)
                     /locus_tag="VFI55_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET7075299.1"
                     /translation="MQPGGDMSALLAQAQQMQQKLLEAQQQLANAEVHGQAGGDLVKV
                     VVKGSGELVAVQIDPKVMDPADIETLQDLIVGAMADASSQVTKMAQERLGALAGGLGG
                     RPAQPPAPAPEL"
     gene            1800..2561
                     /locus_tag="VFI55_14625"
     CDS             1800..2561
                     /locus_tag="VFI55_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3717 family N-acetylmuramoyl-L-alanine
                     amidase"
                     /protein_id="HET7075300.1"
                     /translation="MDLPVSRRVGIRMVIGAVVAASTIIIPTATATPSNIAGMVVFID
                     PGHNGSNDASISRQVPTGRGGTKDCQTTGTATNAGYMEHTFNWDTALRVRAELNALGV
                     RTAMSRGDDTGVAPCVDARADMANSLHPNAVLSIHADGGPPSGRGFHVNYSAPPLNQT
                     QAGPSVQYARIMRDQLAASGIPPSNYIGQDGLYGRSDLAGLNLAQYPSILVECGNMKN
                     PADSALLESPDGRQKYADALVRGVAGFLATQGQAR"
     gene            complement(2577..3014)
                     /locus_tag="VFI55_14630"
     CDS             complement(2577..3014)
                     /locus_tag="VFI55_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7075301.1"
                     /translation="MGQVSAASTILLNVESAVALTAVADYQKVRPKILPPQYSEYQVL
                     QGGQGQGTVAKWKLQATKSRVRDVQVTVDVAGHTVIEKDANSSMVTNWTVAPAGPGSS
                     VTVKTTWNGAGGVKGFFEKTFAPLGLKRIQAEVLANLKKELEG"
     gene            3106..4506
                     /locus_tag="VFI55_14635"
     CDS             3106..4506
                     /locus_tag="VFI55_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7075302.1"
                     /translation="MVPVPGSALSAHAAGVDRLMASYRSIPATSAVRLAKPTSNLFRA
                     RAKRDSRGLDTSGLTGVLGVDPENRTADVAGMCTYEDLVAATLPYGLSPLVVPQLKTI
                     TLGGAVTGLGIESASFRNGLPHESVLEMDILTGAGELLTASRGEHPDLFRAFPNSYGT
                     LGYSTRLRIELEPVAPFVTLRHIRFRSLPEMVAAMERIVDTGGQGGTPVDYLDGVVFS
                     AHESYLCVGRRTTTPGPVSDYTGKDIYYQSIQHDAAGADATKDDRLTIHDYLWRWDTD
                     WFWCSRAFGVQNPRLRRWWPRRYRRSSVYSKLIAMDQRFGLSDRVEKRKGRPARERIV
                     QDIEVPVEQTCEFLAWFLDNVPITPIWLCPLRLRDHEGWPLYPIRPDHTYVNIGFWSS
                     VPAAAAGSSTGSINRTIEAKVSELEGHKSLYSDSFYSREEFDELYGGEAYSTAKKAYD
                     PDSRLLDLYAKAVQRR"
     gene            4503..5813
                     /locus_tag="VFI55_14640"
     CDS             4503..5813
                     /locus_tag="VFI55_14640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7075303.1"
                     /translation="MTTTGERNRATTARLSMAEVLEIFTATGQRPLKFTAYDGSTAGN
                     ADAALGLDLRTPRGATYLATAPGELGLARAYVSGDLQTYGVHPGDPYQLLKTLTDRVQ
                     FRRPSALVLANVVRSIGLERLLPVAPPPQETPPRWRRVAAGVMHSKTRDAEAIHHHYD
                     VSNTFYEWVLGPSMTYTCAVYPNAHATLEQAQDNKYRLIFEKLRLQPGDRLLDVGCGW
                     GGMVRYAARHGVRTIGATLSAEQAKWAQQAIQDEDLTGLAEVRHSDYRDIDEAEFDAV
                     SSIGLTEHIGVRNYPAYFAFLRSKLRTGGLLLNHCITRHDNTSTSFAGGFTDRYVFPD
                     GELTGSGRIVTEIQDAGLEVLHEENFRHHYAMTLRDWCRNLVEHWDDAVDEVGLATAK
                     VWGLYMAASRVAFEQNNLQLHHVLAANVDPGGGDSLPLRPWWQP"
     gene            complement(5818..7644)
                     /locus_tag="VFI55_14645"
     CDS             complement(5818..7644)
                     /locus_tag="VFI55_14645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunits gamma/tau"
                     /protein_id="HET7075304.1"
                     /translation="MALYRKYRPATFAEVVGQEHVTEPLSIALDSGRINHAYLFSGPR
                     GCGKTSSARILARSLNCVQGPTPHPCGVCDSCTALAPNAPGSIDVVELDAASHGGVDD
                     TRELRDRAFYAPAQSRYRVFIVDEAHMVTTAGFNALLKIVEEPPEHLIFIFATTEPEK
                     VLPTIRSRTHHYPFRLLPPRTMRGLIGRICEQEGVVVDDAVYPLVIRAGGGSPRDTLS
                     VLDQLLAGAEGNHVTYQRALGLLGATDVALIDDAVDALAASDAAALFGAVESVIDAGH
                     DPRRFATDLLERFRDLIVLQAVPDAISRGVVDAPEDVLDRMREQATRIGPATLTRYAE
                     VVQAGLGEMRGATAPRLLLEVVCARLLLPSAGDTESALLQRVERIETRLRMSIPTPES
                     DALPPPAAAVEPAAAPARPRKATAAAVSRPAPKPKPAPEPPPEPAPEPAPAADPAPGE
                     PNAAAVRTMWPTVRDKVRQRSRTTEVMLAGATVRAVEDNTLVLTHASGPLAKRLSEQR
                     NADVIADALKDALGVNWRVRCEVGAAAPAAPPPPAGQEPPATEAADADSARRAERDER
                     DEEEYMLAEAGRSEPSEPRRDPEEVALELLQNELGARRIDNG"
BASE COUNT         1313 a         2687 c         2514 g         1300 t
ORIGIN      
        1 ccaacccaag gaccgcggcc acttacgcgg cccgcatcgt cgggctcgcg gccatgttgg
       61 gcgcgaccgt cggctcgatg gttgcgctcg ccggaacggt cgacgcggcc ccggggatgc
      121 acggcgaccc ggccgccgcc gcgcgctact ggcgctacca acggcaggac ctcgactgcg
      181 gggagatggc ggtggccgac gtggtgggcc agatcaccgg ccaggagccc accgaggacg
      241 aggtcaccgg tgcggcggaa agcatcccga gcgtgagcca tcccggaccg atctacaagc
      301 cctccggcaa aaccaaaaac ttggacctgc cggtgctact ggcgcactac gggattccgg
      361 ccggcgcggt ccacaccaac atcgacgcca tcgggcagga cttggatcag gggcacaagg
      421 tgatcgtcgg ggtcaacgac aagaccatct ggaacgagcc gggcgaccgc acccagcaga
      481 accacttcgt ggtggtcacc ggcatcgaca ccaatgccaa tgtggtgcac ctcaacgaca
      541 gcggcatcaa ggccggccgc gatgagcagg tttccatcgc caccttcgaa acggcgtggg
      601 ccaccgacga taacttcgct gtcgtgacca agtgaaaggc agtttgcccg agggccggcg
      661 ctccagccat ctgtctaacg ccggatttga tagcgcatgc gctatcaaat tccacggtcg
      721 cctacgcccc cggagaggcc gcgtcagacc atggcgcggc ggccggtgag cgcgcggccc
      781 agcgtcagct cgtcggcgaa ctccaagtca ccgcccatcg gcagcccgga cgcgatccgc
      841 gtgacggtca ggccggggat gtcgcgcagc atgcggacca ggtaggtggc cgtcgcctca
      901 ccctcggtgt tgggatcggt ggcgatgatc acctcggtga tgtcgacgtc gtcgacgcgc
      961 tcaccgatgc ggctcagcag ctcgcggatt cgcagctgat cggggcccac tccggacaac
     1021 ggatccagcg caccgcccaa cacgtggtag cggccgcgga attcgcgggt gcgctcgacg
     1081 gcctggatgt ccttcgcctc ctcgacgacg cacacctgcg aaccgtcccg gcggggatct
     1141 gcgcagatcc ggcaccgctc gtcgtcggag acattgccgc acaccgcaca gaaccgcaca
     1201 ccgtcgcgga ccttccccag caccgcggta agcctgtcga tttccggcgg ctcgaccgac
     1261 agcaggtgga acgcgatgcg ctgggcactc ttgggcccga tacccggcag cttgccgagc
     1321 tcgtcgatca gatcctggac gggcccctcg aacaattcac agctccggcg ccggcgccgg
     1381 cggttgggca ggtcggccgc ccaggccacc ggccagcgca ccaagccgct cctgcgccat
     1441 tttggtgact tgactggaag cgtcggccat ggctccgacg atcaaatcct gcaaggtctc
     1501 gatgtccgcg gggtccatga ccttggggtc gatctgcacc gcgaccaact cgccactgcc
     1561 tttgacgacg accttgacca aatcccctcc ggcttgaccg tgcacctcag cgttcgcgag
     1621 ctgttgctgg gcttccagta gcttttgctg catctgctgc gcctgggcga gcagcgccga
     1681 catgtcccct ccgggttgca tgacaatccc ctcgcatctt gatctcgagt tggtctcgcc
     1741 tgtaggtgtc gggcgattcg aaacacccag cgtagaccgc gcgacgttac ctttgcgccg
     1801 tggacctacc agtgagcagg cgtgtcggaa tcagaatggt gatcggagca gtggttgctg
     1861 cttcgaccat catcatccca acagcgaccg cgaccccttc caatatcgcc ggcatggtcg
     1921 ttttcatcga ccccggccac aacggctcga acgacgcttc catctctcgc caggtgccca
     1981 cgggtcgcgg tggcaccaag gactgccaga ccaccggaac ggcgaccaac gccggctata
     2041 tggagcacac cttcaactgg gacaccgcgt tgcgggtccg cgctgaattg aacgcgctgg
     2101 gcgtccgcac cgccatgtcc cgcggcgacg acacgggtgt ggcaccctgc gtcgacgcgc
     2161 gcgccgacat ggccaactcg ttgcacccca acgcggtcct gagcatccac gccgacggcg
     2221 gaccgccctc gggccgggga tttcacgtca actactcggc ccccccgctc aaccagacgc
     2281 aggccgggcc gtcggtgcag tacgcccgaa tcatgcgcga tcagctggca gcctcgggca
     2341 taccgccgtc caactacatc ggccaggacg gtctgtacgg gcgctcggat ctggccggcc
     2401 tgaacctggc gcagtatccc tcgattctgg tcgaatgcgg caacatgaag aatccggcgg
     2461 attcggcgct gctggaatca cccgacggcc ggcagaaata cgccgacgcc ctggttcgcg
     2521 gggtcgcggg tttcctggcc acccagggcc aggcgcgctg acccggccta ctccgcttag
     2581 ccttccaact ctttcttcag attggccagg acctcggcct gaatccgctt cagccccaag
     2641 ggcgcaaagg tcttttcgaa gaatcccttg acgccgcccg cgccgttcca agtggtcttc
     2701 accgtgacac tggatccggg tccggcagga gccaccgtcc agttggtgac catggacgag
     2761 ttcgcatcct tctcaatgac cgtgtggccg gccacatcta cggtgacctg cacgtcgcgc
     2821 acccgtgact tggtggcctg cagcttccac ttggccaccg tgccctgccc ctgtccgccc
     2881 tgcagcacct ggtattcgct gtactgcgga ggcaggatct tcgggcgcac cttctgatag
     2941 tcggcaaccg cggtaagagc gaccgcggac tcgacattta acaagattgt gctggctgcg
     3001 ctcacctgtc ccatcaggcg cgactcctct tcagatgtct gtgtgatgtg gtgtgatgct
     3061 gcccgcgcgc gggtgcacgg gttgtttacg aagactaggg tatatgtggt gcctgtccct
     3121 ggatctgcac tctcggctca cgcagcaggc gttgaccggt tgatggcgag ttatcgatcc
     3181 atccccgcga cgtccgccgt ccgactcgcg aaacccacgt caaacctctt ccgcgcccgc
     3241 gccaaacgcg attcgcgcgg cctggacacc tcggggctga ccggtgtcct cggtgtcgat
     3301 cccgaaaacc gcaccgccga cgtcgccggc atgtgcacct acgaggacct ggtggccgcg
     3361 actctgcctt acggcctttc acctctggtg gtcccccaat tgaagaccat caccctcggc
     3421 ggcgcggtca ccggcttggg catcgagtcg gcatcgtttc gcaatggtct gccacacgaa
     3481 tcggtgctgg agatggatat cctcaccggc gctggcgaat tgctcaccgc atcccgtggc
     3541 gagcaccccg accttttccg cgcttttccc aattcctatg ggacgctggg atattcgacc
     3601 aggctgcgga tcgagctgga gcccgtcgcg ccgtttgtga cgctgcggca catccgattc
     3661 cgctcattgc ccgagatggt cgcggcaatg gaacgcattg tcgacaccgg cgggcagggc
     3721 ggcaccccgg tggactatct ggacggcgtg gttttcagcg cccacgaaag ctacctgtgc
     3781 gttggcaggc ggaccacgac cccgggaccg gtcagcgact acaccggaaa agacatctat
     3841 taccagtcaa ttcagcatga cgccgccggt gcagatgcca ccaaagatga ccggctgacg
     3901 attcacgact acctgtggcg ctgggacacc gactggtttt ggtgctcgcg cgcgttcggc
     3961 gtgcagaacc cgcggctgcg gcgctggtgg ccgcgccgtt atcggcgcag cagcgtctac
     4021 tcgaagctca tcgccatgga ccagcgtttc ggtctgtccg accgggtcga aaagcgtaaa
     4081 ggccgccccg cgcgggagcg aatagtgcag gacatcgagg tgccggtcga acagacctgc
     4141 gaattcttgg cgtggttcct ggacaacgtg cccatcacac caatctggct gtgtccgctg
     4201 cggctgcgcg atcacgaagg ctggccgctg tatccaattc ggccggatca cacctacgtc
     4261 aacatcgggt tctggtcttc ggtgccggcc gccgccgcgg gaagctcaac gggctcgatc
     4321 aaccggacga tcgaggcgaa ggtgagcgaa ctcgaggggc acaagtcgct gtactccgac
     4381 tccttctaca gccgcgagga gttcgacgag ctgtacggcg gcgaggccta cagcacggcc
     4441 aagaaagcct acgatcccga ctctcgtctc ctcgatctct acgcaaaggc ggtccaacgg
     4501 cgatgactac caccggagag cgcaaccgcg ccaccacggc ccggctcagc atggccgagg
     4561 tcctggagat cttcaccgct accgggcagc gcccgctgaa gttcaccgcg tacgacggca
     4621 gcaccgccgg caacgcggat gccgcgctgg gcctggatct gcggacgccc cgcggcgcaa
     4681 cctacctggc cacggcccca ggcgaactcg gcctggcccg cgcgtacgtg tcgggtgacc
     4741 tgcagacgta cggcgtgcat cctggcgatc cgtaccagtt gctcaagacg ctcaccgaca
     4801 gggtgcaatt cagacggccg tcggctctgg tgctcgccaa tgtcgtccgg tcgatcgggc
     4861 tcgagcgatt gctgccggtc gcaccgccgc cgcaggagac accgccgcgg tggcggcggg
     4921 tcgctgccgg ggtaatgcac agcaagaccc gtgacgccga ggccatccac caccactacg
     4981 acgtctccaa cactttctac gaatgggtgc tcgggccgtc gatgacctac acctgcgcgg
     5041 tctaccccaa tgcccacgca acgctggaac aggcccagga caacaagtac cggctgattt
     5101 tcgagaagtt gcggctacag ccgggcgacc gtttgctcga cgtcggctgc ggctggggcg
     5161 gcatggtgcg ttacgccgcg cgccacggcg tccggacgat cggcgccacc ttgtcggccg
     5221 agcaggcgaa gtgggcgcaa caggcgattc aagacgaaga cctgacgggc cttgctgaag
     5281 tgaggcactc cgactaccgc gacatcgacg aggcagagtt cgacgccgtc tcctcgatcg
     5341 ggttgaccga gcacatcggt gtgcggaact acccggccta cttcgccttc ctcaggtcca
     5401 aactgcgcac cggcgggcta ctgctcaatc actgcatcac ccgccacgac aacacgtcga
     5461 cctcctttgc cggcggattc accgaccgtt acgtgttccc cgacggggag ttgaccggct
     5521 ccggacgcat cgtcaccgag atccaggacg ccggtttgga ggtgttgcac gaggagaact
     5581 tccggcacca ctacgccatg acgctgcgcg actggtgccg caacctcgtc gagcactggg
     5641 acgacgcggt cgacgaggtc ggtttggcca ccgcgaaggt gtggggtctg tacatggcgg
     5701 cctcacgagt ggcctttgag cagaacaacc tgcagttgca tcacgtgctc gcggccaacg
     5761 tcgatccggg cggtggcgac agcctgccgc tgcgcccgtg gtggcagccc tagccagcta
     5821 gccgttatcg atgcggcggg cgcccagctc attttgtagc agctcgagcg cgacctcttc
     5881 ggggtcgcgc cgcggctccg atggctcgct gcggccggct tcggccagca tgtattcctc
     5941 ttcgtcgcgt tcgtcgcgct cggcgcgccg agccgaatcc gcatcggcag cctcggtcgc
     6001 cggcggttcc tgtccagcgg gcggcggcgg cgccgcgggt gccgccgcgc cgacctcgca
     6061 acgcacccgc cagttcaccc caagcgcgtc ctttagcgcg tcggcgatga cgtcggcgtt
     6121 gcgctgttcg gacagccgct tggccagcgg ccccgatgca tgggtcagca ccagcgtgtt
     6181 gtcctctacc gcgcggacgg tggcacccgc cagcatcacc tcggtggtgc gactgcgttg
     6241 gcgcaccttg tcgcgcaccg tgggccacat ggttcgcacc gcggccgcgt tgggttcgcc
     6301 gggggcggga tctgcggcag gcgccggttc gggggccggt tccggcggtg gttcgggagc
     6361 cggcttgggc ttcggcgcgg gcctggaaac agccgcggcg gttgccttgc gtggccgcgc
     6421 gggggcagcg gccggctcga cggccgctgc gggcggcggg agcgcatcgc tctccggggt
     6481 aggaatggac atgcgcagcc gggtctcgat ccgctcgacg cgctgcagca gcgccgactc
     6541 ggtgtcgccg gccgacggca gcagcagccg cgcacagacg acctcgagca gcaggcgcgg
     6601 cgccgtcgcg ccgcgcatct cccccaggcc ggcctgcacc acttcggcat agcgggtcag
     6661 tgtcgccggc ccgatgcgag tcgcctgctc acgcatccgg tccagcacgt cctcgggcgc
     6721 atccaccacg ccgcggctga tggcgtccgg aaccgcttgc aggacgatca ggtcgcggaa
     6781 ccgttcgagc agatcggtgg caaatcgccg cgggtcgtga cccgcgtcga tcaccgattc
     6841 gaccgcaccg aacaacgccg cggcatctga ggcggcaaga gcgtcaacgg cgtcgtcgat
     6901 cagcgcgacg tcggtggcgc ccagcagccc cagcgcgcgc tgataggtca cgtggttgcc
     6961 ctcggctcca gccagcagtt ggtccagcac cgagagcgtg tctcggggag aaccaccgcc
     7021 ggcgcggatc accagcggat acaccgcatc gtcgacgacg acgccctcct gctcacagat
     7081 ccgcccgatc aatccccgca tggtccgcgg cggcagcagc cggaacgggt agtgatgggt
     7141 gcgcgaacgg atcgtcggca gcaccttctc cggttcggtg gtggcgaaga tgaagatgag
     7201 gtgttcgggt ggttcctcga cgatcttgag cagcgcgttg aaccccgccg tggtcaccat
     7261 gtgcgcctcg tcgacgatga acacgcggta gcgcgactgc gccggagcgt agaacgcgcg
     7321 gtcccgaagc tcgcgggtgt cgtccacgcc gccatggctg gcggcatcga gttccaccac
     7381 gtcgatgctg ccgggcgcat tcggcgccaa cgcggtgcag gagtcgcaaa ctccgcacgg
     7441 gtggggtgtc ggcccctgca cgcagttcaa cgaccgcgcc aggatgcgcg ccgacgacgt
     7501 ctttccgcag ccgcgcgggc cggaaaacaa atacgcgtgg ttgatccgac cggagtccag
     7561 ggcgatggac agcggttcgg tgacgtgctc ctgccccacc acctcggcga aggttgccgg
     7621 tcgatacttg cggtacaggg ccacggtcag caggctaccg gcgcaggctg acgatcgtcg
     7681 tggtggccat cgagtgtgaa ttcacggcgt cgagtgcgaa cacaggtcgc gatttcgccg
     7741 gatttcccgc cctcagctca cactggacgc ggcggaggcc agcatcgact ccgtagccgc
     7801 cagcagcgcg cagg
//
LOCUS       DASSGQ010000337        24778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_236505,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000337 DASSGQ010000000
VERSION     DASSGQ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 24778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24778
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236505"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2598)
                     /locus_tag="VFI55_14650"
     CDS             complement(<1..2598)
                     /locus_tag="VFI55_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HET7075305.1"
                     /translation="MQLISAESTELFVGPPEAPLQLVRVTVSGCAEPAPVRVDGDGLR
                     GEAVASGTGAEQEVIEVPVTVARPVVGERRVARVHAGPARQSFEFTFEFIVAEPGWTM
                     FMISHFHYDPVWWNTQGAYTSEWTEDPPGRARQTNGFALVHAHLEMARREPEYKFVLA
                     EVDYLKPYWDTHPEDRADLRRFIAEGRVEVMGGTYNEPNTNLTSPETTIRNLVHGIGF
                     QRDVLGADPATAWQLDVFGHDPQFPGMAADAGLTSSSWARGPHHQWGPAHGGSEGGDV
                     DRMQFSSEFEWISPSGRGLLTHYMPAHYSAGWWMDSSTSLGEAEDATYALFDQLKKVA
                     LTRNVLLPVGTDYTPPNKWVTAIHRDWAARYTWPRFVCALPREFFAAVRAELADRGDE
                     PSPQTRDMNPIYTGKDVSYIDTKQANRAAENAVLDGERFAVFASLMAGAEYPQAAMAK
                     AWVQLAYGAHHDAITGSESDQVYLDLLTGWRDAWELGRAARDNSLGLLSGALEGPEHS
                     VVVWNPLPHERTDIVTARLDAPSAAGVRVLDADGTELPAHVEHDGRSVTWLARDVPSL
                     GWRAFRLVAGGAAAGWTPVRDREIANEQYRLAVDPSRGGGVRSLVELAQGGRQLIAEG
                     RVGNELAVYEEYPSHPTQGEGPWHLLPKGPVVCSSESAAEVQAYHGPLGQRLVVRGRI
                     GGLLRYTQTLTLWRGVARVDCRTVIDEFTGEDRLLRLRWPCPVPGAMPVSEVGDAVVG
                     RGFALLHDGPQSVDTAEHLWTLDNPAYGWFGLSSAVRVRVGDALRAVSVAEVVSPAET
                     VSAPLARELMVALVRAGVTATCSGADQPRYGHLDVDSNLPDVRIALGGPTRNAFANAV
                     LAQADPVYAA"
     gene            2683..3213
                     /locus_tag="VFI55_14655"
     CDS             2683..3213
                     /locus_tag="VFI55_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7075306.1"
                     /translation="MADLTTQLADQLDWHWRAQLRPRLDGLTDDEYFWQPVADCWTVH
                     PDGSVDFTFPAPSPAPFTTIAWRMAHVIVGVFAMRNHHHFRGPPADYRSWEYATDAAT
                     ALRQLDESYAAWISGVRSLSAADLTRRCGAAEGPYANHPLGELILHINREAIHHGAEI
                     ACLRDLYLHTTVNQED"
     gene            3216..3809
                     /locus_tag="VFI55_14660"
     CDS             3216..3809
                     /locus_tag="VFI55_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7075307.1"
                     /translation="MPALAPPVTDERHALREFLAYHQSAYFAVSYGLTDEQARSTPSV
                     SALSIGGLVKHATGMQRTWMTRVAVAPDEPPKDTRPFEEIAKEFGDQHVMRPDETLAG
                     LLKAFEAQNATSLRLVETADLAAPVPVPQDVPWFPKNQPAWSVRWVILHVINELARHS
                     GHADIIRETIDGATMYELIAGLEGWEIEGWVKPWARR"
     gene            3871..4794
                     /locus_tag="VFI55_14665"
     CDS             3871..4794
                     /locus_tag="VFI55_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7075308.1"
                     /translation="MVTNSGIARRETPEGPVELYYEDLGDPAAPPVLLIMGLGAQLPM
                     WPDGFCAQLVGAGYRVIRFDHRDTGLSAKMHGLRARGSIFRRMGRYLLGKPSSVPYTL
                     IDMTGDVVALLDHLGITRAHVVGASLGGMIAQILAGSEPDRVASLGIIMSTTGKPLSA
                     LPAWRVIKLAFDPPAKDAPAEDKLDFEVRNVAVFNGPNFLPSESELRKRVQQLADRSS
                     YPQGARRQFDAVLGTGSLLRYSKAITAPTVVLHGSADPMVRLRNGRAIAEAIPGARFV
                     VVDGMGHDLPEPVWRPVIEALTENFALAESR"
     gene            4847..6193
                     /locus_tag="VFI55_14670"
     CDS             4847..6193
                     /locus_tag="VFI55_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015292344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HET7075309.1"
                     /translation="MSSTKHREVAKLDRVPLPVEAARVAATGWHVLRTAGRVVSTLPA
                     KGPWQQKVIKQLPQTFADLGPTYVKFGQIIASSPGAFGESLSREFRGLLDRVPPADTK
                     EVHELFAEELGRDPSELFAQWEEEPFASASIAQVHYATLHSGEEVVVKIQRPGIRRRV
                     AADLQILKRFAQTAELAKVGRRLSAQDVVADFAGNLAEELDFRLEAQSMEAWVSHLHA
                     SPLGANIRVPQVHWDYTSERVLTMERIHGIRIDDVAAIREAGFDGVELVKALLFSVFE
                     GGLRHGLFHGDLHAGNLYVDAQGRIVFFDFGIMGRIDPRTRWLLRELVYALLVKKDHA
                     SAGKIVVLMGAVGTTKPEAEAAKDLERFATPLTMQTLGDMSYADIGRQLSTLADAYDV
                     KLPRELVLIGKQFLYVERYMKLLAPKWQMMSDPQLTGYFANFMVEVSREHQSDVEV"
     gene            6203..7108
                     /locus_tag="VFI55_14675"
     CDS             6203..7108
                     /locus_tag="VFI55_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7075310.1"
                     /translation="MEIRTGSATSGDLKLYYEDMGDIDDPPVLLIMGLGAQLLLWRTG
                     FCEKLVGQGLRVIRYDNRDVGLSDKTEYAGAGGPLVPRLLRFWFGLRGEASYSLEDMA
                     DDAAALLDHLGIARAHIVGASMGGMIAQIFAARFAERSKSLAVVFSSNNRRFLPPPAP
                     RALLAVLKGPPPGSPLEVIVDNAVRVTNITGSPRYRAPEEKVRADAVESYERSYYPWG
                     VARHFSAVLASGNLLRYDRRIAVPTVVLHGLADKLMRPSGGRAVARAINGARLELFDG
                     MGHDLPEQLWDQVINVLTSNFAKAS"
     gene            7200..8060
                     /gene="mmaA1"
                     /locus_tag="VFI55_14680"
     CDS             7200..8060
                     /gene="mmaA1"
                     /locus_tag="VFI55_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycolic acid methyltransferase MmaA1"
                     /protein_id="HET7075311.1"
                     /translation="MAKLRPYFEDSQAAYDISDDFFGLFLDPTWVYTCAYFERDDMTL
                     QEAQLAKLDLALDKLNLEPGMTLLDVGCGWGAALVRAVEKYDVNVIGITLSHNHCERS
                     KARLAAIPTTRHAEARLQGWEEFDEKVDRIVSFEAFDAFHKERYSAFFERSYDILPDD
                     GRMLLHSLFTYDRRWLHEQGIALTMSDLKFVKFLRESIFPGGELPSEPDIVDNAKNAG
                     FSVEQIQLLQPHYTKTLNMWAANLEANRDLAIAVQSERVYDNFIHYLTGCAERFRRGL
                     INVGQFTLTK"
     gene            8188..9051
                     /locus_tag="VFI55_14685"
     CDS             8188..9051
                     /locus_tag="VFI55_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane mycolic acid synthase family
                     methyltransferase"
                     /protein_id="HET7075312.1"
                     /translation="MAKDLTPHFDDVQAHYDLSDDFFRLFLDPTQTYSCAYFERDDMT
                     LEEAQLAKIDLALGKLGLRPGMTLLDVGCGWGATMRRAIEKYDVNVVGLTLSKNQAAH
                     VQETFDEMESARSKRVLLAGWEQFDEPVDRIVSIGAFEHFGHDRYDDFFTMADGVLPA
                     DGVMLLHSITGLTGPQCVERGIPLTFEMARFIKFIVTEIFPGGRLPSIEMVADHSSKA
                     GFRLTRRQSLQPHYAKTLDLWAAALEARRDEAIDIQSEEVYERYIRYLTGCANAFRTG
                     YIDVNQFTLEK"
     gene            9212..10687
                     /locus_tag="VFI55_14690"
     CDS             9212..10687
                     /locus_tag="VFI55_14690"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7075313.1"
                     /translation="MDNFEVVIVGAGFGGIGAAIQLRRLGYDKIAILDRAADLGGTWH
                     VNTYPGLAVDIPATTYSYWFEPNPYWSRVFPPGAELQRYAENVADKYDVRRFMRFNTT
                     VECARWDEDERLWQVTLADGESLTARFLITATGFLSLPRKPRIPGIESFAGKVIHTTA
                     WDHGYDLAGRRIGIIGTGATATQLIPELAASAGELTVYQRTAIHVVPRIDFPIPVWLQ
                     KVFARVPLVQRAFRLVTDAFFELVAATVLHHRGLRRLEIAASDAAKINRFIWIRDKEL
                     RRKVTPDYEFGCKRPTWSNSYYRTLTKPHVHLETDTIQRIDPDGIVTADGRKTMIDTL
                     VLATGFDLWDANFPAIEVIGRSGRNLGKWWRENRFQAYQGLSTPYFPNLLGLASPNAF
                     SGLSFFNTIEYQMRHMDRLFGELQRQGATEFEVTEEANARFNDRMTKLVQKSLFSLGD
                     CATARSYYFNPDGEPTLLRPTSTRNAIRDASRFPLTDYQFR"
     gene            10885..11781
                     /gene="mmaA4"
                     /locus_tag="VFI55_14695"
     CDS             10885..11781
                     /gene="mmaA4"
                     /locus_tag="VFI55_14695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymycolate synthase MmaA4"
                     /protein_id="HET7075314.1"
                     /translation="MAEQPTGPTKARTRSEDIQAHYDISDDFFALFQDPTRIYSCAYF
                     EPPDLTLEEAQYAKIDLNLDKLDLKPGMTLLDIGCGWGTTMRRAVEKYDVNVVGLTLS
                     KNQLARSEQVLGAIDTDRSREVRLQGWEDFNEPVDRIVSIEAFEHFGHENYDDFFKRC
                     FDMMPEDGRMTVQSSVSYHPFEMAARGKKLSFETARFIKFIITEIFPGGRLPSTVMMV
                     EHGEKAGFIVPEPLSLRPHYIKTLRIWGDTLESNREKAIAVTSEEVYDRYMKYLRGCE
                     HYFADEMLDCSLVTYLKPGAVT"
     gene            11874..12572
                     /locus_tag="VFI55_14700"
     CDS             11874..12572
                     /locus_tag="VFI55_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET7075315.1"
                     /translation="MQYLALLISGERERTPDEGAAEMAAYQAFHAKAASAIRGGDALS
                     PAATAVRITGGPDAPTITDGPFAEGAEVAGGYYLFEAENLDEAIALARDIPAAKHGAV
                     EVWPVFHSFEPSRSLTGDDWLALLLEPPASAHTPGSPEWEAMAARHGEFQASAGDHVI
                     AGAALHDQSTATTVRVRGGEVSVTDGPYVEGAEVASGFYLLGAQDRDGAVKIASLIPA
                     SAVELRQLAGVSSL"
     gene            12581..13813
                     /locus_tag="VFI55_14705"
     CDS             12581..13813
                     /locus_tag="VFI55_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7075316.1"
                     /translation="MSNLDGVFRREWGPAVAALARWSGDLTVAEDAVQEACAEALRTW
                     PGDGLPDNPGGWLVTVARNRARDRLRRESVRPGKELAALEGYVDNIRARTDHTDPHPV
                     VDDELRMMFTCAHPALDRQSQLALTLRLVSGLTVGEIARALLQTEAAIGQRITRAKNK
                     IRHANIPLRVPPAELLPERTPHVLSCIYSVFTEGYWSTGGPSAIRDELCDEGVRLSGE
                     LCTLMPQELEAHALAALVLLHDSRRATRVDAAGMLVPLDEQDRRRWDRGRISRGLDRL
                     RQAQGSTGPYLPQAVIAALHATAPSWEQTDWATICAAYDRLLRLTDSPVVRANRALAV
                     GLRDGPDAGLAALDKVAHDPRLGRSNLVATVRADLLRRAGRPGEAVTWYRVALESNGS
                     EPGRDFLRRRIAECGGSV"
     gene            complement(13916..14623)
                     /gene="rplA"
                     /locus_tag="VFI55_14710"
     CDS             complement(13916..14623)
                     /gene="rplA"
                     /locus_tag="VFI55_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET7075317.1"
                     /translation="MSKTSKAYRAAAEKVDRANLYTPLEAAKLAKETSSTKQDATVEV
                     AIRLGVDPRKADQMVRGTVNLPHGTGKTARVAVFAVGEKAEQAQAAGADIVGSDDLIE
                     KIQGGFLDFDAAIATPDQMAKVGRIARVLGPRGLMPNPKTGTVTPDVAKAIADIKGGK
                     INFRVDKQANLHFVIGKASFDEKNLAENYGAALDEVLRLKPSSSKGRYLKKITVSTTT
                     GPGIPVDPSITRNFAEA"
     gene            complement(14706..15140)
                     /gene="rplK"
                     /locus_tag="VFI55_14715"
     CDS             complement(14706..15140)
                     /gene="rplK"
                     /locus_tag="VFI55_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET7075318.1"
                     /translation="MPPKKKKKVTGLIKLQIVAGQANPAPPVGPALGQHGVNIMEFCK
                     AYNAATENQRGTVIPVEITVYEDRSFTFALKTPPAAKLLLKAAGVSKGSAEPHKTKVA
                     KVSWDQVREIAETKKADLNANDIDAAAKIIAGTARSMGITVQ"
     gene            complement(15196..15948)
                     /gene="nusG"
                     /locus_tag="VFI55_14720"
     CDS             complement(15196..15948)
                     /gene="nusG"
                     /locus_tag="VFI55_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET7075319.1"
                     /translation="MTTFDGDPSAGEAVGLKETAEQAEVAETSATAEGSVQPESAEDV
                     DPAAALKADLRSRPGDWYVVHSYAGYENKVKANLETRVQNLDVGDYIFQVEVPTEEVT
                     EIKNGQRKQVNRKVLPGYILVRMDLTDDSWAAVRNTPGVTGFVGATSRPSALALDDVV
                     KFLLPRGATKKAAKGAASTAAAAETGGLERPIVEVDYEVGESVTVMDGPFATLPATIN
                     EVNVEQQKLKVLVSIFGRETPVELTFSQVSKI"
     gene            complement(15976..16470)
                     /gene="secE"
                     /locus_tag="VFI55_14725"
     CDS             complement(15976..16470)
                     /gene="secE"
                     /locus_tag="VFI55_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET7075320.1"
                     /translation="MSDEGDAANDAARDGGDTEDGSSTSGRTAVVTKSATRPQRPTGK
                     RSRQRVGDADADVESPDEVEVSKGDKAKKSAKSDTKTAGKAAKAAKAKKPRKSGDRPP
                     NPFVFVYNYLKQVVAEMRKVIWPNRKQMLTYTSVVLAFLAFMVALVGGADFGLAKLVM
                     LVFG"
     gene            complement(16570..16642)
                     /locus_tag="VFI55_14730"
     tRNA            complement(16570..16642)
                     /locus_tag="VFI55_14730"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16607..16609),aa:Trp,seq:cca)
     gene            complement(16804..17304)
                     /gene="hadC"
                     /locus_tag="VFI55_14735"
     CDS             complement(16804..17304)
                     /gene="hadC"
                     /locus_tag="VFI55_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(3R)-hydroxyacyl-ACP dehydratase subunit HadC"
                     /protein_id="HET7075321.1"
                     /translation="MALKTDIRGMSWIYPDYFEVGREQLRAFARAIKCDNAAYFDEAA
                     AAELGYDGLVAPLTFVSIFAKIIQDDFFRNVDVGMETMQIVQVDQRFVYNKPVRVGDK
                     LFGRMDVHSVDERFGADIVVTKNTCANERGEVLLAAYTTLMGHEGDDSIQLRWDKETG
                     QVVRTG"
     gene            complement(17308..17736)
                     /gene="hadB"
                     /locus_tag="VFI55_14740"
     CDS             complement(17308..17736)
                     /gene="hadB"
                     /locus_tag="VFI55_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(3R)-hydroxyacyl-ACP dehydratase subunit HadB"
                     /protein_id="HET7075322.1"
                     /translation="MALREFSSVKVGDQLPEKIYPLTRQDLVNYAGVSGDLNPIHWDD
                     EIAKVVGLDTAIAHGMLTMGIGGGYVTSWVGDPGAVTEYNVRFTAVVPVPNDGKGAEL
                     VFSGKVKSLDPESKSVTIALTATTGGKKIFGRAIASATLA"
     gene            complement(17723..18202)
                     /gene="hadA"
                     /locus_tag="VFI55_14745"
     CDS             complement(17723..18202)
                     /gene="hadA"
                     /locus_tag="VFI55_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(3R)-hydroxyacyl-ACP dehydratase subunit HadA"
                     /protein_id="HET7075323.1"
                     /translation="MGLATDIVGMHYRYPDHYEVEREKIREYAVAVQNDDASFLGEDA
                     AAELGYKGLLAPLTFICVFGYKAQSAFFKYANIAVSDAQIVQVDQVLRFAKPIVAGDK
                     LYCDVYVDATREAHGTQIIVTKNIITNEAGEVVQETYTTLAGRAGDDGEVGFSDGAA"
     gene            complement(18250..18417)
                     /gene="rpmG"
                     /locus_tag="VFI55_14750"
     CDS             complement(18250..18417)
                     /gene="rpmG"
                     /locus_tag="VFI55_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET7075324.1"
                     /translation="MASSTDVRPKITLACEVCKHRNYITKKNRRNDPDRLELKKFCPN
                     CGKHQAHRETR"
     gene            complement(18473..18546)
                     /locus_tag="VFI55_14755"
     tRNA            complement(18473..18546)
                     /locus_tag="VFI55_14755"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18510..18512),aa:Met,seq:cat)
     gene            complement(18601..18673)
                     /locus_tag="VFI55_14760"
     tRNA            complement(18601..18673)
                     /locus_tag="VFI55_14760"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18638..18640),aa:Thr,seq:ggt)
     gene            complement(18802..19011)
                     /locus_tag="VFI55_14765"
     CDS             complement(18802..19011)
                     /locus_tag="VFI55_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HET7075325.1"
                     /translation="MKKRVEIEVDDDLVQEAIRRFHLADAREAVYLALRNLLGGEAGA
                     YGEQDEYDEFSDLSVLRPHRSGDGG"
     gene            19120..19833
                     /locus_tag="VFI55_14770"
     CDS             19120..19833
                     /locus_tag="VFI55_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7075326.1"
                     /translation="MSEDRLYFRQLLAGRDFAAGDMFATQMRNFAYLIGDRQTGDCVV
                     VDPAYAAGDLLDALEADDMHLSGVLVTHHHPDHVGGSMMGFQLAGLAELLERAPVPVH
                     VNTHEALWVSRVTGIGLGDLTTHESHDKVGVGDIEIELLHTPGHTPGSQCFLLDGRLV
                     AGDTLFLDGCGRTDFPGGDSDEMYRSLQRLAALPGDPTVFPGHWYSAEPSASLSKVKR
                     SNYVYRAADLDHWRMLMGG"
     gene            19922..20629
                     /locus_tag="VFI55_14775"
     CDS             19922..20629
                     /locus_tag="VFI55_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HET7075327.1"
                     /translation="MSGPVVYTHDEFIGVISMDDGKVNALGPTMQQAINEAIDQADRD
                     NVGALVIAGNERVFSGGFDLKVLTAGLKTGDAQPAIDMLRGGFELAYRVLSYPKPVVM
                     ACTGHAIAMGAFLLSAGDHRVAAHAYNIQANETAIGMTIPWAALALLKLRLTPSAYQQ
                     SAGLAKAFFGETALAAGFIDEIVLPEMVLSRAEEAAREFAGLNQAAHAATKLRARADA
                     LTALRAGIDGIEAEFGV"
     gene            complement(20785..21813)
                     /locus_tag="VFI55_14780"
     CDS             complement(20785..21813)
                     /locus_tag="VFI55_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET7075328.1"
                     /translation="MTVGDAGALAAQHQPADLVLSGGGVKFIGLVGAIAGLMDGGYSA
                     YRVSGVSAGSVVAAISAAASLGDQLTSGQFKELALSVPLREWRDAGPIPVLGSVLGLV
                     RDTALYRGDFAYDWIRSELKNLGVTTFGDLALDDEELPSERRYKVAVTVADLTAAQLV
                     RLPWDYQRLYGLDPDEQLVADAVRASMAIPFFYPPVQLINNLGLTSNLVDGGVLSNFP
                     VDSLDRTDGKCPRWPTFGITVMPKLAEGLDHLDQVFPALRPMRFLKQSALLESLLTTM
                     LVGHDQAHLSQPWVSARTIPIASTDVSVLDFDISRGRLDALYERGYAAARQFLDTWDW
                     QAYLDRFR"
     gene            complement(22055..22570)
                     /locus_tag="VFI55_14785"
     CDS             complement(22055..22570)
                     /locus_tag="VFI55_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET7075329.1"
                     /translation="MEAWDAICARRNVREYKSEPVSDDDLNRIAEAGWRAPSARNRQP
                     WDFVLVTDRAQLQELSTVWRGAGHIASAAAAIVIVVPVPPDERRLVTDNYDVGQATMA
                     MMIAATDLGIGTGHSSVGDQEKARAILGVPDDHLVAFMLGIGYPADRPLTPIHKPDRR
                     PLSDVVHHGRW"
     gene            22684..>24778
                     /gene="recC"
                     /locus_tag="VFI55_14790"
     CDS             22684..>24778
                     /gene="recC"
                     /locus_tag="VFI55_14790"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HET7075330.1"
                     /translation="MVFHLHRAERTDLLADGLGALLANPPTDPFAEELVLVPARGVER
                     WLSQRLSHVLGRRPGGGDGVCAAVAFRSPASLIAEVTGTVDDDPWSPEAMAWPLLEVI
                     DNSLDEPWCATLATHLGHFHTGEEAELRKGRRYAVARRLAGLFASYARQRPRLLIDWS
                     DGNACDLDEDLLWQPQLWRALVDRVEADPPHLRHQKTVARLREGPSDLPPRLSLFGHT
                     RLAYTDMQLLEALAIHHDVHLWLPHPSDELWRALAGTHGAIARRADVSHRAVRHPLLA
                     ALGRDLRELQRGLPADLRTDEYLPGPRDRERPHTLLGWLQSDIAANVVRPQGRKLAAD
                     DRSVQIHNCHGPARQVDVLREVLLGLLQDDPSLEPRDILVMCPDIETYAPLIVADFGL
                     GDVVHGAHPAHRLRVRLADRSPIQTNPLLAVALRLLTLAASRVTASEVLDFAQAAPVR
                     ARFGFTDDDLEGITRWVRQSNIRWGFDQQHRRPYHVDFVHNTWRFGIDRVLAGVAMSD
                     DSHAWIGSTLPLDDVSSNRVQLAGQFAEFVGRLKHVVDSLTGARPLREWLAALTDGIT
                     RLAHVDDSDSWQTSQMQREFGEVLAVQMGGAGARAETLLRLPDIRALLDGHLSGRPTR
                     ANFRTGTLTVCTMVPMRSVPHRVVCLVGLDDGVFPRLGVVDGDDVLARDPMTGERDIR
                     SEDRQLLLDAIGAATE"
BASE COUNT         4156 a         8334 c         8041 g         4247 t
ORIGIN      
        1 ggccgcgtac accggatcgg cctgtgccag aacggcattg gcgaaggcgt tgcgggtcgg
       61 cccgcccagc gcgatccgca cgtcgggcag gttggagtcg acgtcgaggt ggccgtaccg
      121 cggctggtcc gcaccgctgc aggtggccgt gacaccggcg cggaccagcg cgaccatcag
      181 ctcgcgcgcc agcggggccg acacggtctc ggccggcgac accacctcgg ccaccgacac
      241 cgcgcgcagc gcgtcgccca cccgcacccg caccgccgag gacaggccga accagccgta
      301 ggcggggttg tccagcgtcc acaagtgctc cgcggtgtcc accgattgcg gcccgtcatg
      361 cagcagcgcg aaaccccgcc ccacgacggc gtcccccacc tcgctgaccg gcatggcgcc
      421 cggcaccggg cacggccagc gcagtcgcag caggcggtct tccccggtga actcgtcgat
      481 gaccgtgcga cagtccaccc gcgccacccc gcgccacagg gtcagggtct gggtgtagcg
      541 cagcaggccg ccgatcctgc cccgcaccac cagccgttga ccgagcgggc cgtggtaggc
      601 ctgcacctcg gcggccgatt ccgacgagca caccaccggc cccttgggca gcagatgcca
      661 cgggccctcc ccctgcgtcg ggtgcgacgg gtattcctcg tagacggcca gctcgttgcc
      721 cacccggcct tccgcgatca gctggcggcc gccctgggcc agctcgacca gcgaccgcac
      781 cccgccaccg cggctggggt cgacagccag ccgatactgc tcattggcaa tctcgcggtc
      841 gcggaccggc gtccagcccg cggctgcgcc cccggcaacc aggcggaagg cccgccagcc
      901 cagggacggc acgtcgcgcg ccagccaggt gaccgaacgc ccgtcgtgct cgacgtgggc
      961 gggcagctcg gtgccgtcgg cgtcgagcac ccgcactccc gccgccgacg gcgcgtcgag
     1021 ccgcgcggtg acgatgtcgg tgcgctcgtg tggcagcggg ttccacacca cgaccgaatg
     1081 ctcgggaccc tcgagcgcac cggacagcag ccccagcgag ttgtcgcggg cggcgcggcc
     1141 cagctcccag gcgtcgcgcc agccggtcag caggtcgagg tagacctggt cggactccga
     1201 gccggtgatg gcgtcgtggt gggcgccgta ggccagctgc acccacgcct tggccatggc
     1261 ggcctgcggg tactcggcgc ccgccatcag agaagcgaag acagcgaagc gctcgccgtc
     1321 cagcacggcg ttctcggcgg cccggttggc ctgcttggtg tcgatgtacg acacgtcctt
     1381 gccggtgtag atggggttca tgtcgcgggt ctgcggcgac ggctcgtccc cgcggtcggc
     1441 cagttcggcg cgcaccgccg cgaagaactc ccgcggcagc gcgcacacga agcgcggcca
     1501 cgtgtagcgc gcggcccagt cgcgatggat ggcggtcacc cacttgttcg gcggggtgta
     1561 gtcggtgccg acgggcagca gcacgttgcg tgtcagcgcg accttcttga gctgatcgaa
     1621 cagcgcgtag gtggcgtcct cggcctcgcc cagcgaggtc gacgagtcca tccaccaacc
     1681 cgccgaatag tgcgccggca tgtagtgggt cagcaggccg cgccccgacg gggagatcca
     1741 ctcgaactcg ctgctgaact gcatccggtc gacgtctccg ccttccgagc cgccgtgggc
     1801 cgggccccac tgatggtgcg ggccccgggc ccacgaactc gacgtcaggc ccgcgtcggc
     1861 ggccatcccg gggaattgcg ggtcgtggcc gaacacgtcg agctgccacg cggtggccgg
     1921 gtcggcaccc agcacgtcac gctgaaaacc tatgccgtgc accaggtttc ggatggtcgt
     1981 ttcaggactg gtcagattgg tgttgggttc gttgtaggtg ccgcccatca cctcgacgcg
     2041 gccctcggcg atgaacctgc ggaggtcggc gcggtcctcg gggtgggtgt cccagtacgg
     2101 cttgaggtaa tccacttcgg ccagcacgaa tttgtactcg ggctcgcggc gggccatttc
     2161 caggtgcgcg tggaccagcg cgaagccgtt ggtctgccgg gcccggccgg gcgggtcctc
     2221 ggtccattcg ctggtgtagg cgccctgcgt gttccaccag accgggtcgt agtggaagtg
     2281 gctgatcatg aacatcgtcc acccgggctc ggccacgatg aattcgaagg tgaactcgaa
     2341 cgactggcgg gcggggcccg cgtgcacccg cgcgacccgg cgctcaccga cgacgggacg
     2401 ggcgaccgtg acaggaacct cgatgacctc ctgttcggcg cccgttcccg aggccacggc
     2461 ttccccccgc agaccgtcgc cgtcgacgcg gaccggcgcc ggctcggcac aaccggacac
     2521 ggtgacgcgc accagctgca gcggcgcttc gggcggcccg acgaaaagct cggtcgactc
     2581 cgccgaaatc agctgcatgc ccgcacttta cggcggcccc acgacccgag gaagccgtgg
     2641 cgtgatggaa gcctgtcacc cattgctgga atgctcggtg gcatggctga cctgaccaca
     2701 caactggccg accagcttga ctggcattgg cgcgcacagc tgcgcccccg cctggacggc
     2761 ctgaccgacg acgagtactt ctggcagccg gtggccgact gctggaccgt tcatcccgac
     2821 gggtcggtgg acttcacgtt ccccgcgccc agcccggcgc cgttcaccac catcgcctgg
     2881 cgcatggccc acgtcatcgt cggcgtattc gccatgcgca accaccacca tttccgcgga
     2941 ccgcccgccg actaccgaag ctgggaatac gccaccgacg ccgccactgc cctgaggcaa
     3001 ctcgacgaga gctacgccgc ctggatcagc ggtgtgcggt cgctgtccgc ggccgacctg
     3061 acccgccgct gcggggcggc cgaagggccg tacgccaacc acccgctcgg cgaactgatc
     3121 ttgcacatca accgggaggc gatccaccac ggtgccgaaa tcgcttgcct ccgagacctt
     3181 tacctgcaca caaccgtcaa tcaggaggat tgagaatgcc cgccctcgcc ccgcccgtca
     3241 ccgacgaacg ccatgccctc cgcgagtttc tcgcctacca ccaaagcgcg tatttcgccg
     3301 tctcctacgg ccttaccgat gaacaagccc ggtccacacc ctcggtcagc gcgctgtcga
     3361 tcggcgggct ggtcaagcat gccaccggga tgcagcgcac ctggatgacg cgagtcgcgg
     3421 tcgcgccgga cgagccaccc aaagacaccc gcccgttcga ggagatcgcc aaggagtttg
     3481 gcgaccagca tgtgatgcgt cccgacgaga cgctggccgg gctgctgaaa gccttcgagg
     3541 cccaaaacgc gacctcgctg cggctggtgg aaaccgccga cctcgccgcc ccggtgcccg
     3601 tcccccagga cgtcccctgg ttccccaaga accagccggc ctggtcggtg cgctgggtga
     3661 tcctgcacgt gatcaacgag ctggcccgcc actccgggca cgccgacatc atccgggaga
     3721 ccatcgacgg ggccaccatg tacgagttga tcgccgggct ggagggctgg gagatcgaag
     3781 gatgggtaaa gccctgggca cgcaggtagc aaggctcggc cggtagcaag gctcggccgg
     3841 tagctaggct cggcgggtag aaaagagagc gtggtcacca acagcggaat cgcccggcgc
     3901 gagacgcccg aaggccccgt cgagctgtac tacgaggatc tcggcgaccc cgcggcccca
     3961 cccgtactgc tgatcatggg cctgggcgcc cagctgccga tgtggccgga cggcttctgc
     4021 gcgcagctcg tcggcgccgg ctaccgcgtc atccgcttcg accaccgcga caccgggctg
     4081 tcggccaaaa tgcacggact gcgggcccgg ggctccatct tccggcggat gggccgttat
     4141 ctgctgggca agcccagttc ggtgccctac acgttgatcg acatgaccgg ggacgtggtg
     4201 gcgctgctcg atcatctggg catcacgcgc gcccatgtgg tcggcgcgtc gctaggcggc
     4261 atgatcgccc aaatcctggc cggcagtgag ccggaccggg tggcgtcgct gggcatcatc
     4321 atgtcgacca ccggcaagcc gttgtcggcg ttgccggcct ggcgggtgat caagctcgcc
     4381 ttcgatccgc cggccaagga cgccccggcc gaggacaagc tggacttcga ggtgcgcaac
     4441 gtcgcggtgt tcaacgggcc gaacttcctg ccgtccgaat ccgagctgcg aaagcgtgtg
     4501 cagcaactgg cggataggtc cagctatccg cagggcgcgc ggcgccaatt cgatgccgtg
     4561 ctgggcaccg gcagtctgct ccgctacagc aaggcgatca ccgcgcccac tgtggtgctg
     4621 cacggttcgg ccgacccgat ggtgcgcctg cgcaatggac gcgcgatcgc cgaggcgatt
     4681 cccggcgcgc gattcgtcgt ggtcgacggg atgggacacg acctccccga accggtctgg
     4741 cggccggtca tcgaggccct caccgagaat ttcgcgctcg ccgagtcccg gtgagcgcaa
     4801 tcacaggaat tgggacttga cagtgccgat ttggcacact gctgagatga gttccaccaa
     4861 acaccgcgag gtggccaaac ttgaccgggt gcccctgccg gtcgaagcgg cccgggtagc
     4921 cgccaccggc tggcacgtcc tgcgcaccgc cggccgcgtc gtgtccactc tgccggccaa
     4981 agggccgtgg cagcagaagg tgatcaagca gctcccgcag accttcgccg acctgggccc
     5041 gacctacgtg aagttcggac agatcatcgc ctccagcccc ggcgcgttcg gcgagtcgct
     5101 gtcccgcgaa ttccgcggcc tgctcgaccg ggtcccgccc gccgacacca aggaagtgca
     5161 cgagctcttc gccgaggagc tgggccgcga cccttccgag ctgttcgccc aatgggagga
     5221 agagccgttc gcgtccgcct ccatcgccca ggtgcactac gcgaccctgc acagtggcga
     5281 ggaagtcgtc gtcaagattc agcgtcccgg catccgccgc cgcgtcgcag ccgacctgca
     5341 gatcctcaag cgcttcgccc aaaccgccga attggccaag gtaggccgtc ggctgtcggc
     5401 gcaggacgtc gtggccgact tcgccggcaa cctggccgaa gagctggact tccggctcga
     5461 ggcgcagtcg atggaggcct gggtctcgca cctgcacgcg tcgccgctgg gcgccaacat
     5521 ccgggtaccg caggtgcact gggactacac ctccgagcgg gtgctgacga tggagcgaat
     5581 ccacgggatc cgcatcgatg acgtcgccgc catccgcgag gccgggttcg acggcgtcga
     5641 gctggtgaag gcgctgctgt tcagtgtgtt cgagggcggg ctgcgccacg ggctgttcca
     5701 cggcgacctg cacgccggca acctctacgt cgacgcccag ggccgcatcg tgttcttcga
     5761 cttcggcatc atgggccgca tcgatccccg cacccgctgg ctgctgcgcg agctggtgta
     5821 cgcgctgctg gtgaagaagg accacgcttc ggccggcaag atcgtcgtgc tgatgggcgc
     5881 cgtggggacc accaagccgg aggccgaggc cgccaaggac ctggagcgct tcgccacccc
     5941 gctgaccatg cagacgctgg gcgacatgtc ctatgccgac atcgggcggc aattgtcgac
     6001 actggccgac gcctacgacg tcaagttgcc ccgggagctg gtgctgatcg gcaagcagtt
     6061 cttgtacgtc gagcggtaca tgaagttgct ggcaccgaaa tggcagatga tgtccgaccc
     6121 gcagctgacg ggctactttg cgaacttcat ggtcgaggtc agccgagagc accagtctga
     6181 cgttgaggta tagggcggtc tgatggagat ccgcaccggc agcgctactt cgggcgacct
     6241 gaagctctac tacgaggaca tgggtgacat cgacgaccca cccgtactgc tgatcatggg
     6301 gctgggcgca cagctgttgc tgtggcggac cggcttctgc gagaagctcg tcggccaggg
     6361 cctgcgggtc atccgctacg acaaccgcga tgtcggcttg tccgacaaga ccgagtacgc
     6421 cggcgccggc gggccgctgg tcccgcggct actgcggttc tggttcggcc tgcgcggcga
     6481 ggcgtcctac agtttggaag acatggccga cgacgccgcc gccctgctgg accacctggg
     6541 cattgcgcgc gcccacatcg tcggcgcgtc gatgggcggc atgatcgcgc agatcttcgc
     6601 ggcccggttc gccgagcggt ccaagagcct ggcggtcgtc ttctccagca ataaccgcag
     6661 gttcctgcca ccgcccgcgc cacgcgccct gctggcggtc ctcaaaggcc cgccgcccgg
     6721 ctcgcccctc gaagtgatcg tcgacaacgc ggtgcgcgtc accaatatca ccggcagccc
     6781 gcgctaccgc gcgcccgagg agaaggtccg cgccgacgcg gtcgaaagct acgaacgcag
     6841 ctattacccc tggggcgtcg cccggcactt cagcgccgtc ctggccagcg gaaatctgct
     6901 gcgctacgac cgcaggatcg cggtaccgac cgtggttctg cacggcctgg ccgacaaact
     6961 gatgcggccc tccggcggcc gcgcagtcgc gcgcgccatc aacggcgctc gattggagtt
     7021 attcgacggc atgggtcatg atctgccaga acagctgtgg gaccaggtga tcaacgtact
     7081 gacgagcaat ttcgccaaag ccagctgaag caaacacaat gcggccagaa gggctgcagc
     7141 gtgcggctat cgcgcaaaga cgcgcggctg tacggttggc gttgggagga tgtctaataa
     7201 tggcgaagct gagaccgtat ttcgaagact cgcaagcggc gtacgacatt tcggacgact
     7261 ttttcgggct gtttctggac cccacctggg tctacacgtg cgcatacttc gagcgcgacg
     7321 acatgacgct ccaagaggcg cagctcgcca agttggacct ggccctggac aagctgaacc
     7381 tcgagcccgg gatgacgctg ctcgacgtgg gatgcggctg gggcgcggcg ctggtccggg
     7441 ccgtcgaaaa atacgacgtc aacgtcatcg gcatcacgct gagtcacaat cactgcgagc
     7501 gcagcaaggc gcggctggcg gccatcccga cgactcggca cgccgaggcg cggctacagg
     7561 gctgggaaga gttcgacgag aaggtcgacc ggatcgtctc gttcgaggcg ttcgacgcct
     7621 tccacaagga gcggtacagt gcgttcttcg aacgctcgta cgacatcctc cccgacgacg
     7681 gccggatgct gctgcacagc ctgttcacgt acgaccgccg gtggttgcac gagcagggca
     7741 ttgctctgac gatgagcgat ctgaagttcg tcaaattcct gcgcgagtcg attttccccg
     7801 gtggcgaact tccttccgag cccgacatcg tcgacaatgc gaagaacgcg ggattctcgg
     7861 tggagcaaat ccagctgctg caaccgcatt acacaaagac attgaacatg tgggccgcaa
     7921 acttggaggc caaccgggat ctcgccatcg cggtccagtc cgaacgggtt tacgacaatt
     7981 tcatccatta cctgaccggc tgcgcggagc ggttccgcag ggggctcatc aatgtggggc
     8041 aattcaccct caccaagtga ggtgaccgac ctcgtgggat tggatgtcgg gattggatac
     8101 cgtgccctga gcatgccggg gggaaatttc ggtgcgtttg gcctgagcgc caagcggccg
     8161 gcacggtcca gaaaggcaaa caacgtcatg gccaaggatt tgacgccgca cttcgacgat
     8221 gtgcaggccc actacgacct ctccgacgac ttcttccgcc tcttcctgga ccccacccag
     8281 acctatagct gcgcgtactt cgagcgcgac gacatgacgc tggaagaggc gcagctcgcc
     8341 aagatcgacc tggcgctggg caagttgggg ttgcgccccg gcatgacgct gctcgacgtc
     8401 ggctgcggct ggggcgccac catgcggcgc gccatcgaaa agtacgacgt caatgtcgtc
     8461 ggcctgacgt tgtcgaagaa ccaggccgcg cacgtgcagg aaacgttcga cgaaatggag
     8521 agcgcgcgca gcaagcgggt gttactggcc ggctgggaac agttcgacga accagtcgac
     8581 cgcattgtgt cgatcggcgc gttcgagcac ttcggtcacg accgctacga cgacttcttc
     8641 acgatggccg acggcgtcct gccggccgac ggggtgatgc tgctgcactc gatcaccggg
     8701 ctgaccggtc cgcaatgcgt cgaacgcggc ataccgctga cgttcgagat ggcgcggttc
     8761 atcaagttca tcgtcaccga gatcttcccc ggcggccggt tgccgtcgat cgagatggtg
     8821 gcggaccact cgtcgaaggc cggtttccgg ctgacccggc gccagtcgct gcagccccac
     8881 tacgccaaaa ccctcgactt gtgggcggca gcgctcgagg cgcgccgcga tgaggccatc
     8941 gacattcagt ccgaagaggt ctacgagcgc tacatcaggt atctgaccgg ctgcgccaac
     9001 gcgtttcgga ccggctacat cgacgtcaat cagttcaccc tggagaagta gcgcatttat
     9061 ggagatgtga cggagctcaa accaatccgt gcctatgctc cgcgagcgcc aaggcggact
     9121 ctctactaaa ctctcagcta atcatgttcg tcgtgggggc agctggcgaa acaccagttg
     9181 cccgggtcca cgaaaggcgc tttgacacaa catggacaat tttgaagtcg taatagtagg
     9241 tgcgggattc ggcggcattg gcgcagcgat ccagctccgg cggctgggat acgacaagat
     9301 cgcgattctc gatcgcgcgg ccgatctcgg cgggacctgg cacgtcaata cctaccccgg
     9361 cctggccgtc gacatcccgg ccaccaccta ttcgtactgg tttgagccca atccgtactg
     9421 gtcacgggtg ttcccgccgg gtgccgaact gcaacggtac gccgagaacg tcgccgacaa
     9481 gtacgacgtg cgccgcttca tgcggttcaa cacgaccgtc gagtgcgcgc gctgggatga
     9541 ggacgagcga ttgtggcagg tgacgctcgc agacggcgaa agcctgacgg cgcgcttcct
     9601 gatcaccgcg accggcttcc tgtcactgcc gagaaaaccg cggatccccg gaatcgagag
     9661 cttcgcgggc aaagtgatcc acaccacggc atgggaccat ggttacgacc ttgccgggcg
     9721 tcgcatcgga atcatcggaa ccggggcgac cgctacgcag ttgatccccg aactggccgc
     9781 cagcgcgggc gagctgaccg tgtaccaacg cacggcgatc catgtggtgc ccaggatcga
     9841 tttcccgatc ccggtctggc tgcaaaaggt gttcgcccgg gtgcccctgg tgcagcgagc
     9901 cttccgcctg gtgaccgacg ccttcttcga gctggtagcg gccactgtgc tacaccaccg
     9961 agggcttcgg cgactcgaga tcgccgcgtc ggacgccgcc aagatcaacc ggttcatctg
    10021 gatccgcgac aaggagctgc ggcgcaaggt cacccccgac tacgaattcg gctgcaagcg
    10081 gcccacctgg tccaacagct actaccgcac cctgaccaag ccacacgtgc atctggagac
    10141 cgacaccatc caacggatcg atcccgacgg aatcgtcacc gccgacgggc gcaagacgat
    10201 gatcgacacg ctggtgctgg ccaccggatt cgacctgtgg gacgccaatt tccccgccat
    10261 cgaagtgatc gggcgcagtg ggcgcaacct gggaaagtgg tggcgcgaga acaggtttca
    10321 ggcatatcag ggcctgtcca ccccgtactt ccccaacctg ctgggcctgg ccagcccaaa
    10381 tgcgttttcc ggcctgtcat tcttcaacac gatcgaatat cagatgcggc acatggaccg
    10441 gctgttcggg gagctgcagc gccagggcgc aactgaattc gaggtgaccg aagaagcaaa
    10501 tgcgcgtttc aacgatcgga tgaccaagct tgttcagaag tctctcttct ccctcggaga
    10561 ctgcgccacc gcgcggtctt actacttcaa ccccgatggc gagccgacac tgctgcggcc
    10621 cacgtcgacg cgcaacgcaa tacgcgacgc atcacgattc ccgctcaccg attaccaatt
    10681 ccgctgaccg gctgggctgg ggttcggcta cggagcgcgc cgtcgagaag tacgacgtgg
    10741 ttaccgacat gttccgggtc cgccgagccc agcgcccacc cgcttgaatg cgcctcgcca
    10801 ttgggtatgg ttgcacgtca cgacaaccag cgatgcgtgc gcgcgtcgta ggcagtgacc
    10861 ttcatccagg agagcaagac gaaaatggct gagcaaccga ctgggccgac gaaggcgcgg
    10921 acgcgctctg aggacattca ggcgcactac gacatctccg acgatttctt cgccctgttc
    10981 caggacccga cgcggatcta cagctgcgct tatttcgagc cgccggacct cacgctcgaa
    11041 gaggcccagt acgccaagat cgacctcaac ctggacaagc tggacctcaa gccgggcatg
    11101 acgctgctgg acatcggctg cggctggggc accaccatga ggcgcgccgt tgagaagtac
    11161 gacgtcaacg tcgtcggcct gacgctctcc aagaatcagc tcgcccgttc cgagcaggtg
    11221 ctcggggcca tcgataccga ccgctcacgc gaggtgcgac tgcagggctg ggaggatttc
    11281 aacgagccgg tagaccgcat cgtgtcgatc gaggccttcg agcacttcgg gcacgaaaac
    11341 tacgacgact tcttcaagcg ctgtttcgac atgatgcccg aggacggccg gatgaccgtc
    11401 caaagcagcg tcagctacca cccgttcgag atggccgccc gcggcaagaa gctcagcttc
    11461 gagacggccc gtttcatcaa gttcatcatc accgagatct tcccgggcgg gcggctgcct
    11521 tccaccgtga tgatggtcga acacggcgag aaggccggtt tcattgtccc cgagccgctt
    11581 tcgttgcggc cgcactatat caagacgctg cgcatctggg gcgacacgct ggagtccaac
    11641 agggaaaagg ccatcgccgt gacctccgaa gaggtctacg accggtacat gaagtatctg
    11701 cgcggctgcg agcactactt cgccgacgag atgctcgact gcagcctggt gacctacctc
    11761 aagccgggcg ccgtcacctg atttctccgc gggtgtcgga ttagggctgc ggcgttcgtc
    11821 gaacaggtag agagtggagc acaaggctcc ctcactaccg gaggtgacga caaatgcagt
    11881 acttggctct gttgatcagc ggggagcgcg agcgcacgcc cgacgagggg gcggcggaga
    11941 tggcggcata tcaggcgttc cacgccaagg ccgcctcggc gatccgcgga ggtgacgccc
    12001 tgtcgccggc ggcaaccgcg gtgcgcatca ccgggggccc ggacgcgccg accatcaccg
    12061 acggcccatt cgccgaaggt gccgaagtgg ctggcggcta ttacctgttc gaagccgaaa
    12121 acctcgatga ggcaatcgct ttggcgcgtg acatcccggc ggccaagcat ggcgcggtgg
    12181 aggtgtggcc ggtgttccac tcgtttgaac cgtcccgcag cctgaccggc gacgactggc
    12241 tcgcactgct gctggagcca cccgcgtccg cgcacacgcc cggctcaccg gagtgggagg
    12301 cgatggcggc acgccacggt gagttccagg cctccgcagg cgatcacgtg attgccggcg
    12361 ccgcgttgca cgaccagtcc acggcgacga cggtgcgggt gcgcggcggc gaagtcagcg
    12421 tgaccgacgg cccgtacgtg gagggcgccg aggtggcgag cggcttttac ctgctcggcg
    12481 cccaggaccg cgacggggcc gtcaagatag cgtcgctgat cccagcttcc gccgtcgagc
    12541 tgcggcagct ggccggggtg tcctccctat agctgcaccc gtgtccaacc tggacggcgt
    12601 cttccgccgg gaatggggac ccgccgtcgc cgcgctcgcg cggtggtccg gcgacctcac
    12661 cgttgctgag gacgccgtcc aggaagcctg cgccgaggcg ctgcgcacct ggcccggcga
    12721 cggcctgccc gataaccccg gcgggtggct ggtgaccgtc gcgcgcaacc gcgcccggga
    12781 tcgactacgc cgcgaatcag tgcgccccgg aaaggaattg gcggcgttgg aaggctacgt
    12841 ggacaacatc cgggcgcgca ccgatcacac cgacccgcat ccggttgtcg acgacgagct
    12901 gcggatgatg ttcacgtgcg cgcacccggc gctggaccgg cagtcgcagc tggcgctcac
    12961 gctgcggttg gtatccggtc tgacggtcgg cgagattgcc cgcgcgttgc tgcagaccga
    13021 ggcggctatc ggtcagcgaa tcacacgcgc caagaacaag attcgtcacg ccaacatccc
    13081 gctgcgggtg ccacccgccg agctgctgcc cgagcgcaca ccacacgtgc tcagttgcat
    13141 ctactcggtg ttcaccgagg ggtactggtc gacggggggc ccttcggcga ttcgcgacga
    13201 actgtgcgac gaaggggtcc ggctgagcgg ggagctgtgc acgctgatgc cacaggagct
    13261 ggaggcgcat gccttggccg cgctggtgct gctgcacgat tcgcgacggg caacccgagt
    13321 ggacgctgcc gggatgctgg tgccgctcga tgaacaggac cgccgtcgct gggatcgcgg
    13381 ccgtatctcg cgtgggctgg atcggttgcg acaggcccag ggatcgacgg gaccctacct
    13441 gccgcaggcg gtgatcgccg cgctacacgc gacggcgccg tcgtgggagc agaccgactg
    13501 ggcgaccatc tgcgcggcgt acgaccggct gctgcggctg accgactccc cggtggtccg
    13561 cgccaaccgc gcgctggcgg tcggtctccg cgacggtccc gacgccggcc tggccgccct
    13621 ggacaaggtg gcccatgatc cacggctggg ccgctcgaac ctcgtcgcca ccgtgcgcgc
    13681 cgacctgctg cggcgggcgg gacggcccgg cgaggccgtc acgtggtacc gagtcgcgtt
    13741 ggagtccaac ggttccgagc cgggccgcga cttcttgcgg cggcgcatcg ccgagtgcgg
    13801 cggatcggtg tgaacctcac gccgacagtg aatcttgcgg cgagacacgc cggcccgccg
    13861 tcgcaggatg cccagtgggc gactaaggcc gaaaagacta ctacggccga aaagactagg
    13921 cctcggcgaa gttgcgggtg atggacgggt ccaccggaat gcccgggccc gtggtcgtcg
    13981 acacggtgat cttcttcaga tagcggccct tcgacgacga cggcttgagc cgcagcacct
    14041 cgtcgagcgc ggcgccgtag ttctcggcca ggttcttctc gtcgaacgac gccttgccga
    14101 tgacgaagtg caggttggcc tgcttgtcca cgcggaagtt gattttgccg cccttgatgt
    14161 cggcgatggc tttggcgacg tcgggggtga cggtgccggt cttggggttc ggcatgaggc
    14221 cgcgcgggcc cagcacgcgg gcgatgcgac cgaccttggc catctgatcg ggggtggcga
    14281 ttgcggcgtc gaagtccagg aagccgccct gaatcttctc gatcaggtcg tcactgccga
    14341 cgatgtcggc tcctgcggcc tgggcctgct cggccttctc gccaacggcg aacaccgcga
    14401 cccgggccgt cttaccggtg ccgtggggca ggttgaccgt gccgcggacc atctggtctg
    14461 ccttgcgcgg gtcgacgccc agccggatcg ccacctcgac ggtcgcgtcc tgcttggttg
    14521 acgacgtctc cttggcgagc ttggccgcct ccagcggggt gtacaggttg gcccggtcca
    14581 ccttctcggc ggcggcgcgg tatgccttgc tggtcttgct catttgatct gtccaatctg
    14641 tgttgggtcg tggttagtaa gcgggccgaa gctggccctc ccacgcatga ttaatttgtc
    14701 gagggctatt ggacggtgat ccccatcgac cgcgcagtgc cggcgatgat cttggcggcc
    14761 gcgtcgatgt cgttggcgtt gagatcggct ttcttggttt cagcgatctc ccggacctga
    14821 tcccagctga ccttggcgac ctttgtcttg tgcggctccg cggacccctt ggacaccccg
    14881 gcggccttga gtagcagctt ggcggcgggg ggcgtcttga gcgcgaacgt gaagctgcgg
    14941 tcctcgtaga ccgtgatctc caccgggatg acggtgccgc gctggttctc cgtggcggcg
    15001 ttgtacgcct tgcagaactc catgatgttg acgccgtgct gaccgagcgc gggtcccacc
    15061 ggcggggccg ggttggcctg acctgccacg atctgcagct tgatcagccc ggtgactttc
    15121 ttcttctttt tcgggggcat ggggtgtcga ttgttccttc ctgaatttcg gctttggttt
    15181 tggttgcctc aggggctaga tcttggagac ctggctaaag gtcagttcca ccggtgtttc
    15241 gcggccgaag atcgacacca gaaccttgag cttctgctgt tcgacattga cctcgttgat
    15301 ggtggccggc aacgtggcaa acgggccgtc catgaccgtc accgattcac cgacctcgta
    15361 gtcgacctcg acaatcggac gctccagccc acccgtctcg gcggcagcgg cggtgctggc
    15421 cgcacccttg gcagccttct tcgtcgcccc ccgcggcaag aggaacttca ccacgtcgtc
    15481 gagcgccaga gccgacgggc gcgacgtcgc accgacgaag cccgtaaccc caggggtgtt
    15541 gcgcaccgcg gcccacgagt cgtcggtcaa atccatccgc accaggatgt agccgggcag
    15601 caccttgcgg ttgacctgct tgcgctggcc gttcttgatc tcggtgacct cttcggtggg
    15661 aacctccacc tggaagatgt agtcgcccac gtcaaggttc tgcacgcggg tttccaggtt
    15721 ggctttcacc ttgttctcgt atcccgcata ggagtgcacg acgtaccagt cgccgggcct
    15781 gctgcgcagg tcggccttga gcgcggcggc cgggtcgacg tcctcagccg actccggttg
    15841 cacggagccc tctgcggtcg ctgaggtctc ggcgacctcg gcttgttcgg cggtctcttt
    15901 caggccgacc gcttcacccg cggacgggtc accgtcgaag gtagtcacgg tttcagtcct
    15961 ctctcgatca tttgctcagc cgaacaccag catcaccagc ttggccaggc cgaaatccgc
    16021 cccgccgacc agcgccacca tgaaggcgag aaacgccagc acaactgagg tgtaggtaag
    16081 catttgcttg cggttgggcc aaatcacctt ccgcatctct gcaacgacct gcttgaggta
    16141 gttgtagacg aacacgaagg gattaggcgg acgatccccg gatttcctcg gcttcttggc
    16201 cttagcggcc ttagctgcct tgccggccgt cttcgtgtcc gacttggcac tcttcttggc
    16261 cttgtcgccc ttggagacct cgacctcgtc cggcgactca acgtctgcgt cggcatcgcc
    16321 gacgcgctgc cgggaccgct tgccggtggg ccgttgcggg cgcgtcgcag acttggtcac
    16381 cacggccgtc cgaccgctgg tcgagctccc gtcctcggtg tcgccgccgt cgcgtgcagc
    16441 gtcgttggca gcgtcgcctt cgtcgctcac cgcatgctcc tttgtttgct agttcccgcg
    16501 agccgtgccc cagtgctcgg acacccttcc agtgtccacc atggcacgat actcattgtc
    16561 cgtcttcagc aggggcgaca ggacttgaac ctgcaacctg cggttttgga gaccgctgct
    16621 ctgccagttg agctacgccc cttcgcggtt ggcaggccaa cctgcgctta cctaccaggg
    16681 cttacatgac acgcgcgctc tccgttcggt gatcgccgaa ccgacggaca gcgcgctgta
    16741 atgggaaaac cccgaggtcc gagtgtactc gattccactt gagcgcagga aatccgatac
    16801 cgattacccg gttctgacga cctgcccggt ttctttgtcc catctgagct ggatggagtc
    16861 atcaccctcg tggcccatca gggtggtgta ggccgccaat aagacctcgc cacgctcgtt
    16921 ggcgcaggtg ttcttggtga cgacgatgtc ggcgccgaaa cgctcgtcca ccgaatggac
    16981 gtccatgcgg ccgaagagtt tgtcgccgac gcgcaccggc ttgttgtaga cgaaccgctg
    17041 gtcgacctgg acgatctgca tggtttccat gcccacgtcg acattccgga aaaagtcgtc
    17101 ctggatgatc ttggcgaaga tggacacgaa cgttaacggt gcgacgaggc cgtcgtagcc
    17161 cagttcggcg gccgctgcct cgtcgaagta ggccgcgttg tcgcacttga tcgcgcgcgc
    17221 gaatgcgcga agttgctccc ggccgacctc gaagtagtca gggtatatcc agctcatccc
    17281 gcggatatca gtcttgagcg ccatgggtta ggccaatgtc gccgacgcga tcgcccggcc
    17341 gaagatcttc ttgccaccgg tggtggccgt gagcgcgatg gtcaccgact tgctctcggg
    17401 atcgagcgac ttcacctttc cgctgaagac gagctcggcg cccttgccgt cgttgggcac
    17461 cggcaccacc gcggtgaacc gcacgttgta ctcggtgacc gcaccggggt cacccaccca
    17521 cgacgtcaca tagccgccgc cgatgcccat ggtcagcatg ccgtgggcga tcgcggtgtc
    17581 cagcccaacg accttggcga tctcgtcgtc ccagtggatc gggttcaggt cgcccgagac
    17641 ccccgcgtag ttcaccagat cctggcgggt cagcgggtag atcttctccg ggagctggtc
    17701 gcccaccttg accgaactga actcacgcag cgccatcaga aaatcccact tccccgtcgt
    17761 cgccggcacg gcccgccagg gtcgtgtagg tctcctgcac cacctcacct gcttcgttgg
    17821 tgatgatgtt cttggtcacg atgatctggg tgccgtgcgc ctcacgggtc gcatccacgt
    17881 acacatcgca atacagtttg tcgcccgcga cgatcggctt ggcgaacctc agcacctggt
    17941 cgacctggac gatctgcgcg tcggagaccg cgatgttcgc gtacttgaaa aacgccgact
    18001 gcgccttgta gccgaacacg cagatgaacg tcagcggagc cagcaacccc ttgtagccga
    18061 gctcggccgc ggcgtcctcc ccaaggaacg aggcgtcgtc gttttggacg gctaccgcgt
    18121 actcgcggat cttctcgcgc tcgacctcgt aatggtcggg ataccggtaa tgcatcccga
    18181 caatgtctgt ggccaatccc acgggtctag aacctaccta gtcagtctga gcaaacgctc
    18241 gcggaagagc tagcgtgtct cgcggtgggc ctggtgcttg ccgcaattcg ggcagaactt
    18301 cttcagctcc agccggtcgg gatcgttgcg gcggttcttc ttggtgatgt agttacggtg
    18361 cttgcacacc tcgcacgcca aggtgatctt gggccgcaca tcggtactgg aagccatgtc
    18421 ggttctctct cacatctttc tcgcgtctct cgcgagaccc attgttgtgt tgtagcgatg
    18481 gccggactcg aaccggcgac ctaacgatta tgagtcgttc gctctaaccg actgagctac
    18541 atcgcctctg ggccaccccg atttgggctt ggggccgccg tgcctgctcg gcgtcagccg
    18601 agccccctaa cggaatcgaa ccgttgacct tttccttacc atggaaacgc tctaccgact
    18661 gagctaaggg ggccgttcac ccaagcgacc agtcgtgtcc cgggcctaaa agagggtaca
    18721 gcctggcgag caacgtcacc aaaccacgaa cgtccccgtt gaaaagaagt ttgcgacgac
    18781 gtggcggcgg tgaggagaaa atcacccgcc gtcgccgctg cggtgcggtc gcagaacgct
    18841 gaggtcgctg aactcgtcgt actcgtcttg ctccccgtac gcgccggcct cgcccccgag
    18901 taagttccgc aacgcgagat agacggcctc gcgcgcgtca gccagatgga atcggcgtat
    18961 cgcctcttgg accagatcgt cgtcgacctc gatttccacc ctcttcttca tgcgccaaca
    19021 atacccagcg cgccttcgcc ccgctgtgaa cggcagctct cctttacccg aataccaagt
    19081 cgcgcgcggc cagtcttgaa aaagacctag tctgatcacg tgtcagagga ccggctgtac
    19141 ttccgtcaat tgctcgcggg tcgcgatttc gccgctggcg acatgttcgc gacgcaaatg
    19201 cgcaacttcg cctacctgat cggcgaccgt cagaccggcg actgcgtggt ggtcgacccc
    19261 gcgtacgccg ccggggacct gctcgacgcg ctcgaggcgg acgacatgca cctgtccggc
    19321 gtgctggtga cccatcacca ccccgaccac gtcggcggct cgatgatggg ctttcagctg
    19381 gcgggcctgg ccgagctgct ggagcgcgca cctgtgccgg tgcacgtcaa cacccatgag
    19441 gccctttggg tttcgcgggt caccgggatc ggcctcggtg acctcaccac ccacgagagc
    19501 cacgacaagg tcggcgtcgg cgatatcgaa atcgagctcc tgcacacccc cggtcacaca
    19561 ccaggcagcc aatgctttct gctcgacggc cgcctggtcg ccggcgacac gctgttcctg
    19621 gacggctgcg gacgcaccga ttttcccggt ggcgactccg atgagatgta tcgcagcctg
    19681 cagcggcttg ccgcactccc cggtgatccc accgtgtttc cggggcattg gtattcggct
    19741 gagccgagcg cctcgctatc gaaggtcaag cgttccaact acgtgtatcg cgccgcggat
    19801 cttgatcatt ggcgaatgtt gatgggcggc taagcgaacc acaagtgtct ccaccagctc
    19861 ggcgggagtg cctcaggccg atctgattta atcgacgggc tacccgatca aaggagcaac
    19921 tgtgagcggt ccggtcgtct acacccacga cgagttcatc ggggtcatca gcatggacga
    19981 cggcaaggtc aacgcgctgg gcccgaccat gcagcaagcg atcaatgagg cgatcgacca
    20041 ggccgaccgg gacaacgtcg gggcgctggt gatcgccggc aacgaaaggg tgttcagcgg
    20101 cgggttcgac cttaaggtac tgaccgccgg cctcaagacc ggcgacgcgc agcccgcgat
    20161 cgacatgctc aggggcggct tcgagttggc ctatcgggtg ctgtcctacc ccaagccggt
    20221 ggtgatggcc tgtaccggtc acgcgatcgc gatgggcgcg ttcctgttgt cggccggcga
    20281 ccaccgcgtg gccgcccacg catacaacat ccaggccaac gaaacggcga tcgggatgac
    20341 catcccctgg gcggcgctgg cgctgctgaa gctgcggctc acgccgtcgg cctatcagca
    20401 gtccgccggc ctggccaagg cgttcttcgg ggaaaccgcg ctggccgcag ggtttatcga
    20461 cgagatcgtg ctgcccgaga tggtgctcag ccgcgccgag gaagccgcgc gggagttcgc
    20521 cggcctcaac caggcagccc acgccgcgac caagttgcgg gcccgcgccg acgcgctcac
    20581 ggcgctgcgc gccgggatcg acgggataga agccgagttc ggagtctgag gcgcgctccg
    20641 cacgtattga cgcgctcaaa tcgatgaacg cggtggcctg ttaacggcct ggccaccgcg
    20701 ttgtcatcgc ctcctcgccg ctagtgtgac acgttcacgc cgtgccgtca cgtcactccc
    20761 cggccacact ccagccatca ccgatcagcg gaaccggtcc aggtaggcct gccaatccca
    20821 ggtgtccagg aactgccggg ccgccgcata gccccgctca tagagcgcat ccagacgccc
    20881 gcgggaaatg tcgaagtcca gaacgctcac gtcggtcgac gcgatcggta tcgtgcgggc
    20941 gctgacccag ggctggctca ggtgcgcctg atcgtggccg accagcatgg tggtcaagag
    21001 gctttccagc agggcggatt gtttgaggaa acgcatcggg cgcaatgccg ggaacacctg
    21061 gtccaggtgg tctaagccct cggccagctt gggcatcacg gtgatcccga acgttggcca
    21121 gcgcgggcat ttgccgtcgg tgcggtccag cgaatcgacc gggaaattcg acagcacgcc
    21181 gccgtcgacc agattggacg tcagccccaa gttgttgatc agctggacgg gcgggtagaa
    21241 gaacgggatc gccatcgacg cacggaccgc gtcggcgacg agctgctcgt cgggatccag
    21301 accgtagagc cgctgatagt cccacggcag ccgcaccagc tgcgccgcgg tcagatcggc
    21361 gacggttacc gccaccttgt agcgtcgttc gctgggcagt tcctcgtcat cgagggccag
    21421 atcaccgaac gtcgtcaccc ccaggttctt cagctcgctg cggatccagt cgtaggcgaa
    21481 atcgccgcgg tacaaagcgg tgtcgcggac caggcccaac accgagccca gaacgggaat
    21541 cggccccgcg tcgcgccatt cgcgcaacgg caccgacagc gccagctctt tgaactgccc
    21601 gctggtcagt tggtcgccca gggacgctgc ggccgagatc gccgcgacca ccgatcccgc
    21661 cgagacaccc gacacccggt aggccgaata cccgccgtcc atcagcccgg cgatggcgcc
    21721 aaccagaccg atgaacttga cgcccccgcc ggagagcacg agatcggcgg gttggtgctg
    21781 cgccgccaag gcgccggcgt cgcccacagt catcgggcga aacccattgc aggggaagga
    21841 attttccgcc caccggacgg ggtgatctca ggcctcttca tggccccagc atgcaccgcg
    21901 gcggtccgct gtctcggcca ccacgcgctt tccgaatttg atagcgcatg cgatagcaaa
    21961 aacggcgacg atatatgcct tgggcgccag ccggcgggct tcaccccggg ccggacggtg
    22021 gcgccgaacg attttgacgc cgtgcgccgc gtgatcacca gcgcccgtga tggacgacgt
    22081 cggacaaggg ccgccgatcg ggcttgtgga tcggggtgag cggacgatcg gccggatatc
    22141 cgattccgag catgaacgcc accaggtggt cgtcgggcac gcccaggatc gcacgtgctt
    22201 tctcctggtc gcccaccgac gaatgtcccg tcccgatccc caggtcggtg gccgcgatca
    22261 tcatcgccat cgtggcctgg ccgacgtcgt agttgtcggt gaccagtctg cgttcatccg
    22321 gcgggaccgg caccacgatg acgatcgcgg cggcggccga ggcgatgtga cccgcgcctc
    22381 gccagaccgt ggacagctcc tgcagctgag cgcggtcggt gacgaggacg aaatcccagg
    22441 gctgtcggtt tcttgccgat ggcgcgcgcc agccggcctc ggcgatgcgg ttcagatcgt
    22501 cgtctgagac cggctccgat ttgtactccc tgacgttgcg ccgcgcgcag atcgcgtccc
    22561 aagcttccat ctgacccttc ttgtttctgc ctcgtcctct gccgatggtt ccatgacggt
    22621 cgaacggcgt ttcgacggcg aatcattccc accgtcggcg gggcgttcta tggtggcacc
    22681 gcaatggtct tccatctcca tcgtgccgag cgcaccgacc tgcttgccga cggtctcggc
    22741 gccctgctgg ccaacccgcc gaccgacccg ttcgccgagg aactggtgct ggtgccggcg
    22801 cgcggcgtgg agcggtggct gagccagcgg ctctcgcacg tgctggggcg caggcctggc
    22861 ggcggcgacg gtgtgtgcgc ggcggtggcg tttcgcagtc cggcctcatt gatcgctgag
    22921 gtcaccggca cagtagacga cgacccgtgg tcgccggagg cgatggcgtg gccgctcctg
    22981 gaggtcatcg acaacagcct cgacgagccg tggtgcgcca cgctggcaac gcatttgggc
    23041 cacttccaca ccggcgagga ggccgagctg cgcaagggtc gccgctacgc ggtggcacgg
    23101 cggctggccg gactgttcgc ctcctatgcc cgtcagcgtc cgcgactgct catcgactgg
    23161 tcggacggca acgcgtgcga cctcgacgag gatctgctct ggcagccgca actgtggcgg
    23221 gcgctggtcg accgggtgga ggccgatccc ccgcacctcc gccaccagaa gaccgtcgcg
    23281 cggctgcggg aagggccgag cgacctgccg ccgcggctgt cgctgttcgg gcatacccgc
    23341 ctggcataca ccgacatgca actcctcgag gcgctggcga tccaccacga tgtgcaccta
    23401 tggctgccgc accccagcga cgagctgtgg cgcgcgctgg ccggcaccca cggcgcgatc
    23461 gctcgtcgtg ctgacgtcag ccaccgcgcg gtgcgccatc cgctgttggc ggcactcggg
    23521 cgggacctgc gcgaattgca gcgcggcctg cccgcggatc tgcgcaccga cgaatatctc
    23581 ccaggcccgc gcgaccggga gcgaccgcac acgctgctcg gctggctgca gtccgacatc
    23641 gccgccaatg tcgttcggcc acaagggcgc aagcttgccg cggatgaccg ctcggtgcag
    23701 atacacaact gccatggccc ggcccgtcag gtcgatgtgc tgcgcgaggt gctgctcggc
    23761 ctcctgcaag atgacccgag tctggagcca cgcgacatcc tggtgatgtg tccggacatc
    23821 gagacgtatg cgccgctgat cgtcgccgac ttcgggctcg gggacgtcgt gcacggcgcg
    23881 catcccgctc accggttgcg cgtgcggctc gcggaccgct cgccgatcca gaccaacccg
    23941 ctgctggcgg tggccttgcg gttgttgacg ctggccgcca gcagggtgac cgccagtgag
    24001 gtactcgact tcgcgcaggc cgcgccggta cgcgcccgct tcggtttcac cgacgatgat
    24061 ctggaaggca tcacccgctg ggtccggcag tcgaacatcc ggtggggttt cgaccagcag
    24121 catcggcggc cctatcacgt cgacttcgtg cataacactt ggcgtttcgg catcgaccgg
    24181 gtattggccg gggtggcgat gtccgacgac tcccacgcct ggatcggctc gacgctgccc
    24241 ctcgatgacg tcagcagcaa ccgggttcaa ttggccggcc agttcgccga attcgtcggc
    24301 cggctaaagc atgtggtcga ctcgctcacg ggggcgcggc cgctgcgcga gtggctggcc
    24361 gccctcaccg acggcattac ccggttggcg cacgtcgacg attcggacag ctggcagacc
    24421 agccaaatgc agcgcgagtt cggcgaggtg ctggccgtgc aaatgggcgg ggccggtgcg
    24481 cgggccgaga ccctgctgcg gctgccggat atccgggctc tgctcgacgg gcacctgtct
    24541 ggacgcccca cacgggccaa cttccgcacc ggcacgctga cggtttgcac catggtgccg
    24601 atgcgctcgg tgccgcatcg ggtggtctgc ctggtcggac tcgacgacgg ggtgttccca
    24661 cgcctcggtg tcgtcgacgg tgacgacgtg ctggcccgcg acccgatgac cggggaacgc
    24721 gacatccgtt cggaggacag gcaattgctg ctcgacgcca tcggagcggc cacggaga
//
LOCUS       DASSGQ010000338        12205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1512925,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000338 DASSGQ010000000
VERSION     DASSGQ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12205
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512925"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="VFI55_14795"
     CDS             complement(<1..149)
                     /locus_tag="VFI55_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HET7075331.1"
                     /translation="MDAPELVALLAGRRLAVLTGAGISTDSGIPDYRGPDSPPSNPMT
                     IRQFTS"
     gene            289..2355
                     /locus_tag="VFI55_14800"
     CDS             289..2355
                     /locus_tag="VFI55_14800"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HET7075332.1"
                     /translation="MVSYRDFYNSYSQGFLRVAACTHRTTIGDPAANAASVVRLAREC
                     HDDGVAVAIFPELTLSGYSIEDIVLQDLLLDDVENAIGDIVAASADLLPVLAVGAPLR
                     YRHRIYNTAVVIHRGVVLGVAPKSYLPTYREFYERRQIAPGDGERGVIRIGGSGQVLE
                     APFGPDLLFAASDVPDFVLHVEICEDMFVPIPPSAEAALAGATVLANLSGSPITIGRA
                     EDRKLLARSASSRCLAAYLYAAAGEGESTTDLAWDGQTMIWENGVLLAESERFPRGER
                     RSVADVDTGLLRAERLRMGTFDDNRRHHRTSAESFRRIEFRLDPPGGDIGLLREIERF
                     PFVPADPQRLELDCYEAYNIQVSGLEQRLRALNYPKVVIGISGGLDSTHALIVAARAM
                     DREDRPRSDILAFTLPGFATGERTKRNAVELCRALGVTFSEIDITDTARLMLKEIDHP
                     FAHGEKVYDVTFENVQAGLRTDYLFRLANQRGGIVLGTGDLSELGLGWSTYGVGDQMS
                     HYNVNAGVPKTLIQHLIRWVIASGQFETRVDEVLQSVLDTEITPELVPSGEEEELQSS
                     EAKVGPFALQDFALFQVLRYGFRPSKIAFLAWHAWRDSERGTWPPGYPMDKRPSYSLK
                     EIRHWLQVFMQRFYSFSQFKRSALPNGPKVSQGGALSPRGDWRAPSDMSARIWLDEIE
                     REVPED"
     gene            complement(2371..2925)
                     /locus_tag="VFI55_14805"
     CDS             complement(2371..2925)
                     /locus_tag="VFI55_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET7075333.1"
                     /translation="MAVMALAGKLFRRVRAGERRSPLAGGGFAAPQSITVTSSAFTDG
                     GAMPRSSAGKGVGANTSPALRWAGLPAGTRQVVLIIDDVDVPLPRPLLHTVAVIDPTL
                     DRVAAGALQSGTAGMRFIRADLGRRGYAGPRPIPGHGPHRYRFHVFAIDQPIADGVAT
                     VKALLAVMSGHVLARGVLSGTYER"
     gene            complement(2925..3494)
                     /locus_tag="VFI55_14810"
     CDS             complement(2925..3494)
                     /locus_tag="VFI55_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HET7075334.1"
                     /translation="MTVTSLTFQGKVWVFGDNLNTDAMYPAFAMKMDPPEAARHIFYE
                     IRPGWTDQVSPGDIVVAGKNFGLGSSRPVASLFTELGVAGLVAEEFNSLFFRNAVNAG
                     LPAMTLPDATAVFSEGDTGTFDLTEGTWRNDTTGASGSVPKLPDLVLEIIESGGVMPR
                     LAAQGYLPAELGDLLRSSAVAMRGAGSGA"
     gene            complement(3491..4738)
                     /locus_tag="VFI55_14815"
     CDS             complement(3491..4738)
                     /locus_tag="VFI55_14815"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HET7075335.1"
                     /translation="MGMTIIEKTFARKAGRESVSPGDTVVVDVDMTVLIDLQFATMWI
                     QPSRIHDPDKLAVIMDHAVPAPTIKDATGGRHARKFAADFGIERFYDVGRHGICHQVI
                     AENGLARPGEILACTDSHTCAAGAYNTAARGLGPAEVYSIMCTGTTWFQVAPTVRYEL
                     VGAKPNTVSGKDIFLHIANEYGDAANLNLEFGGPGLAGIPMHDRRTIATQGAEVSADF
                     STFEADDVLARFLDDAGVTGYTTAAPDSDATYHDVRHIDLAKLEPYVARPGTVSRNGL
                     PVSQLGRQKVDQAFIGSCANGQLEDLEIAAEVLRGKTVAPGVRLLITPASQAVYRQAM
                     RLGYLQDLADAGAVITNSTCGACFGYHMGVVGPGEVCITSSTRNFTGRMGSAEAKIFM
                     ASPATVAASAITGYISDPRSVTA"
     gene            complement(4729..5616)
                     /locus_tag="VFI55_14820"
     CDS             complement(4729..5616)
                     /locus_tag="VFI55_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HET7075336.1"
                     /translation="MATTPARRRLKELLDTRELIVAPGVFDGISAHLTKRTGHVAAYM
                     TGAGVAASGFGLPDIGLVTATEMADRVRMIVGALGDIPLIADADTGYGAPMNVARTVV
                     SYDAAGVAAIQLEDQVFPKRCGHLPDKQVVDAAVFEQTLAAALDARSDDGLLVVARTD
                     ARAPLGLEAAIARANRYAQAGADIIFVEAPQDTDEIERIGREVDAPLLINLVLGGMTP
                     LESASRLQELGYAIAIHPSNLLMRATFGMLQSLCELNGTDMAAHLPTSPADFFNLVGL
                     AEWRELDDKYAHTKDRSWA"
     gene            complement(5627..7057)
                     /locus_tag="VFI55_14825"
     CDS             complement(5627..7057)
                     /locus_tag="VFI55_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HET7075337.1"
                     /translation="MTAVHRQPATDPNGPTGRLAGWVADLTLDQVPHSVTERAKHLLL
                     DGLGCALVGARLPWSRIATEAVLGLEGSGGTVVIGTGHTTSAPAAAVLNGTFIQGFEL
                     DDFHPLAPLHSCSLLIPALLSTATTRPETTTGAEFLLGAIAGFEVGPRVGYTLHGTQM
                     LDRGWHSGPVFGTHSAAMASGKLRGLPPGQLEDALGLAGTQSAGLMAAQYEAMSKRMH
                     HGLAARNGFYAAGLAAAGYTGIKRVFEREYGGYLSVFGEGHDPDASLLTGQLGQRWET
                     TIIMVKSYAAMGGLHGAIDAARQLRNAVAPEDISKVDITVGETIYKHGWWLPERPLTP
                     IGAQMNIGYATAAALLDGNVLPDQFTPGRLDSDDIWSLLGATSVHLDETLAHADITTK
                     FRTDVALTTHDGAVHHARVDVPHGAPTDPVTNDELVAKFHALADRVTSRERTEAIERA
                     VVCLEDLDDVDHLIDLLADPVASALD"
     gene            7113..7976
                     /locus_tag="VFI55_14830"
     CDS             7113..7976
                     /locus_tag="VFI55_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7075338.1"
                     /translation="MSTMQEGGVEALTTVHKTSGKASPVSTDAGVPLHRQLFLVLHDE
                     IDRGVIAPGEPLPSEQTLCDQFGVSRITVRRALADLAEQGYIERRQGVGSFVREHGPA
                     DASDALGGGGSYMAGLRQSQFETEVDVVELGTRRPPRAIAEALRSSGELLHIVRVRRQ
                     RRTAEPLMVTDAWLPADLAGTLTESALRRAPLYQLLSDAGIAVGRMRHEITAEIAGPR
                     NAHLLDTAIGAALLRVNRLAFVADAPHHHLSVLLSPNRSRVLLSQTGDELEMADGLTI
                     AHDVARPRANE"
     gene            complement(7966..8583)
                     /locus_tag="VFI55_14835"
     CDS             complement(7966..8583)
                     /locus_tag="VFI55_14835"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET7075339.1"
                     /translation="MPATAAADMALVLFAVFGVLGLGWRSWLQYRRTGSTGFRGVSAG
                     GFTEGVAGVGFVIALIVAVSASILQETKVIQPVKALSVVWVQAVGIVIATAGIVATVY
                     AQLEMGDSWRIGVDEKEVTTLVQTGVFGQIRNPIYTAMFTFGVGIALVTPNLVACASF
                     ILLVAMIQLQVRRVEEPYLLRTHGDAFRAYAASVGRFVPGVGLIR"
     gene            complement(8639..9523)
                     /locus_tag="VFI55_14840"
     CDS             complement(8639..9523)
                     /locus_tag="VFI55_14840"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HET7075340.1"
                     /translation="MAARVCVVGSVNMDSTFDVAALPRPGETVLASSWASAPGGKGGN
                     QAVAAARAGAQVQFVGAVGDDPAADQLRAHLRANGVGLDGTVALTGPSGAAIIVVDAG
                     GENTIVVAPGANGRLTLSAAAAREVIGASDVLLTQLEIPVPTAVAAAREARSAGAVVI
                     VNASPAGPDQGSLAELAAVADVVIANETEADGWPWRPAHLVITLGARGARYIGTDGEF
                     AVPAPVVDAVDTTGAGDVFAGVLAANWPPNPGSPAERLAALRRACAAGALATLTPGAG
                     DCAPGAAAIDNALRDGLS"
     gene            10280..10451
                     /locus_tag="VFI55_14845"
                     /pseudo
     CDS             10280..10451
                     /locus_tag="VFI55_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877948.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
     gene            complement(10484..11245)
                     /locus_tag="VFI55_14850"
     CDS             complement(10484..11245)
                     /locus_tag="VFI55_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016886848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS and ANTAR domain-containing protein"
                     /protein_id="HET7075341.1"
                     /translation="MQRHPENESWFAVEQSSGSDSKVDVGEALADTGPHRVGWFRFYF
                     DDDRWEWSPQVEKMHGYLPGSVAPTTEMVLAHKHPDDYQQIAHTLELIRQSRQAFSSR
                     HRIIDVEGRVHHVVVVGDLLRDDDESVIGTHGFYIDVTPSERARQDEVTAAVARITES
                     RSGIEQTKGMLMLVYGIDELTAFELLKWRSQEANVKLRTLAEQIATDFLELSRGETLQ
                     PRSAYDNLVLTAHLRIDAENQSRDANDIPPSEETG"
     gene            11755..>12205
                     /locus_tag="VFI55_14855"
     CDS             11755..>12205
                     /locus_tag="VFI55_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7075342.1"
                     /translation="MKDLKDGAEPASTADPETAPESAPAEKSPKRTRRPLVWTRFRFG
                     IQSKILVAMLLSSILGVSVIGLIGALSGRSALRQVESERLIELRETSKRQVEALFREV
                     TNSLIVHSGGFRVTEATAELTAVFNQLANATITPAQQQALVTYYDNDM"
BASE COUNT         2030 a         3939 c         4074 g         2162 t
ORIGIN      
        1 gacgtgaact ggcggatggt catgggattg ctcggcggcg agtcgggtcc ccgatagtcg
       61 ggaatgccgg agtcggtgga tatccccgca ccggtcagca ccgcgagacg gcggccggcc
      121 agcagtgcga ccagctccgg agcgtccacg gttcaagagt agacgccgcg cgagcgtgcg
      181 tgtctgcgcg ccgacacgcc ggctgaagcg gcggtttgcg cacgctcacg ggtcgcgtgc
      241 tacccggcgg ccccgggttg acactgctcg ggcgacaatg gataggccat ggtttcttac
      301 agggacttct acaatagcta cagccagggt ttcctccgtg tcgcggcgtg cacgcaccgc
      361 accacgatcg gcgacccggc ggccaatgct gcatcggtcg tgcggttagc ccgcgagtgc
      421 cacgacgacg gggtcgcggt ggcgatcttc cctgagctga cgctgtcggg gtactccatc
      481 gaagacatcg tgctgcaaga ccttttgctc gacgatgttg aaaacgcgat aggcgatatc
      541 gtcgccgcct cggccgactt gctgccggtt ttggccgtcg gcgccccgct gcgctaccgg
      601 caccgcatct acaacaccgc agtggtcatt caccgaggcg tggtgctcgg cgtggcgccc
      661 aagtcctacc tgccgaccta tcgggagttc tacgagcgcc gccagatcgc accgggagac
      721 ggcgagcggg gcgtcatccg cattggcggc tcggggcagg tgctggaggc accgttcggc
      781 cccgatctgc tgttcgccgc atccgacgta cccgacttcg tgttgcacgt cgaaatctgc
      841 gaagacatgt tcgtgccgat tccgcccagc gcggaagcgg cgttggcggg agcgaccgtg
      901 ctggccaatc tctccggtag cccgatcacc attggccgcg ccgaggaccg caagctgctg
      961 gcccgctcgg cgtcgtcgag gtgcctggcc gcctacctct acgccgccgc gggggagggc
     1021 gaatccacca ccgacctggc ctgggacggg cagaccatga tttgggagaa cggcgtcctg
     1081 ctcgccgaat ctgaacgctt ccccaggggc gagcgtcgca gcgtcgccga cgtggacacc
     1141 gggctgcttc gcgccgaacg gctgcggatg gggacgttcg acgataaccg gcgccaccac
     1201 cggacgtcgg ccgagtcgtt tcgccggatc gagttccggc tggacccgcc gggcggcgac
     1261 atcgggctgc tgcgggagat cgagcggttt ccgtttgtgc ccgccgatcc acaacggctg
     1321 gaactggatt gctacgaggc ctacaacatc caggtctccg ggctcgagca acggctgcgc
     1381 gcgttgaact atccgaaggt ggtgatcggg atctcgggcg ggctggactc gacgcatgcg
     1441 ctgatcgtcg ccgcgcgcgc gatggatcgt gaagatcggc cgcgcagcga cattctggcg
     1501 ttcacgctgc ccggcttcgc gactggcgag cgcaccaaac gcaatgcggt cgagctgtgt
     1561 cgtgcgctcg gtgtcacctt ctccgaaatc gacatcaccg acaccgcaag gttgatgctc
     1621 aaggagatcg atcatccgtt cgcacacggc gagaaggtct acgacgtcac cttcgagaac
     1681 gtccaggccg gcctgcgcac cgactatctg ttccggctgg ccaaccagcg cggcggcatc
     1741 gtgctgggca ccggtgatct ctccgagctg gggctggggt ggtcgacata cggggtcggc
     1801 gaccagatgt cgcactacaa cgtcaatgcc ggagtgccca agacgctgat ccaacacctg
     1861 atccgctggg tcattgcgtc cgggcagttc gaaacgcggg tggacgaggt gttgcagtcg
     1921 gtccttgaca ccgagatcac ccccgagttg gttcccagcg gcgaggagga agagctgcag
     1981 agcagcgagg cgaaggtcgg accgttcgcc ctgcaagact ttgcgctgtt ccaggtgctg
     2041 cgctacggat tccggccgtc gaagatcgcg tttttggcct ggcatgcgtg gcgtgattcc
     2101 gagcgcggca cctggccgcc cggatatcca atggacaagc gaccgtctta ctcgctcaag
     2161 gagattcggc actggttgca ggtctttatg cagcggttct attcgttcag ccagttcaag
     2221 cggtcggcgt tgccgaacgg gcccaaggtg tcgcaaggcg gcgcgttgtc gccgcggggg
     2281 gattggcggg ccccgtcgga catgtccgcg cgcatctggc tcgacgagat cgaacgcgaa
     2341 gtgcccgaag actagccgag ccgggtggcc ctagcgttcg taggtgccgg acagcacgcc
     2401 tcgggccagc acgtgtccgc tcatcaccgc gagcagcgcc ttcacggtag cgacgccgtc
     2461 tgcaatcggc tggtcgatcg cgaacacgtg gaatcggtag cggtgcggcc cgtggccggg
     2521 aatcgggcgc ggtcccgcat agccgcgacg acccaaatcc gcgcgaatga atcgcattcc
     2581 cgcagtgcca gactgcaacg cgcccgctgc gactcggtcc agcgtcgggt cgatcaccgc
     2641 gacggtgtgc agcaggggac ggggcagcgg aacgtcgacg tcgtcgatga tgagcacgac
     2701 ctgccgcgtc cccgccggta gccctgccca ccgcaacgca ggtgaggtgt tggcgccgac
     2761 gcccttgccc gcgctggatc gcggcatcgc gccgccgtcg gtgaacgccg agctggtgac
     2821 cgtgatggac tggggcgcgg caaacccgcc tccggccagt gggctgcggc gctcgccggc
     2881 ccgaacgcgg cggaaaagct tgcccgccag tgccattacg gccattacgc gccactcccg
     2941 gcgccgcgca tggcgaccgc gctggagcgc agcaggtcgc cgagttcggc cggcagataa
     3001 ccctgggccg ccaaccgggg catcacgccc ccgctttcga tgatctcgag aaccaggtcg
     3061 ggcagtttgg gtacggagcc cgaggcgccc gtggtgtcgt tacgccacgt gccctcggtc
     3121 agatcgaaag tcccggtgtc accctcgctg aacacggcgg tagcgtcggg cagggtcatc
     3181 gccggcaggc cggcgttgac ggcgttgcgg aagaacagtg agttgaactc ctcggcgacg
     3241 agcccggcca cgccgagttc ggtgaacaat gacgcgactg ggcgagacga gccgagcccg
     3301 aagttcttgc ctgccaccac gatgtcgccc ggcgaaacct gatcggtcca gccggggcgg
     3361 atctcgtaga aaatgtgcct ggccgcctcc ggcgggtcca tcttcatcgc gaaggccggg
     3421 tacatggcgt cggtgttgag gttgtcgccg aacacccaca ccttgccctg aaaggtaagc
     3481 gatgtgaccg tcatgccgtc acgctcctgg gatcgctgat atacccggtg attgccgacg
     3541 ccgcgacggt cgccggtgag gccatgaata tcttcgcctc ggcgctgccc atccgtccgg
     3601 tgaagttgcg ggtgctggac gtgatacata cctcgccggg tccgaccaca cccatgtggt
     3661 agccgaaaca ggcgccgcag gtggaattgg tgatgacggc accggcgtcc gcgaggtcct
     3721 gcaggtagcc cagccgcatg gcttggcggt acaccgcctg cgaggcgggc gtgatcagca
     3781 gccgcacgcc cggggctacc gtcttgccgc gcaacacctc tgcggcgatc tcgaggtcct
     3841 cgagttgacc gttcgcgcac gacccgatga acgcctggtc caccttctgc ctgcccagtt
     3901 gcgatacggg caggccattg cggctgaccg tgcccggccg cgcgacatag ggttccagct
     3961 ttgccagatc gatatgccga acgtcgtggt aggtggcgtc ggaatccggt gcggcggtgg
     4021 tgtatccggt cacgccggcg tcgtcgagga agcgggccag cacgtcgtcg gcttcgaacg
     4081 tgctgaagtc ggccgacacc tcggctccct gggtcgcgat ggtgcgccgg tcgtgcatcg
     4141 ggataccggc caggccgggg ccgccgaact ccaggttcag gttggcggcg tcgccgtact
     4201 cgttggcgat gtgcaggaag atgtccttgc cgctcaccgt attcggcttg gcgccaacga
     4261 gttcgtagcg gacggtgggt gccacctgga accacgtcgt gccggtgcac atgatcgaat
     4321 acacctcggc gggcccaagc ccacgggccg cggtgttata ggcgcccgct gcgcaggtgt
     4381 gagagtcggt gcaggccaat atttctccgg ggcgcgccaa cccgttctcg gcgatcacct
     4441 gatggcagat gccgtgccgg cccacgtcgt agaaacgctc gatgccgaag tcggcggcga
     4501 actttcgggc gtgccgaccg ccggtggcgt ccttgatggt cggggcgggc acggcgtggt
     4561 ccatgatgac cgccagcttg tcggggtcgt ggatccggct gggctggatc cacatggtgg
     4621 cgaactgcag atcgatcaac acggtcatgt cgacgtcgac cacgacggtg tcgccgggcg
     4681 agaccgactc gcgaccggcc ttgcgcgcga aggtcttctc gatgatggtc atgcccatga
     4741 gcggtccttg gtgtgtgcgt acttgtcgtc gagttcccgc cattccgcca agccgaccag
     4801 gttgaagaag tcggcggggc tggtgggcag gtgggcggcc atgtccgtgc cgttgagttc
     4861 gcagaggctt tgcagcatgc cgaacgtcgc gcgcatcagc aggttgctcg ggtggatcgc
     4921 gatcgcatag cccagctcct gtagccgcga cgccgactcc agcggcgtca tcccgccgag
     4981 caccagattg atcagcaggg gagcgtccac ttcgcgtccg atgcgctcga tttcgtcggt
     5041 gtcctgcggc gcctcgacaa agatgatgtc ggcgcccgct tgcgcgtagc ggttggcccg
     5101 cgcgatcgcg gcctccagtc caagcggggc acgggcgtcg gtgcgggcca ccaccagcag
     5161 gccatcatcg gaccgggcgt ccagtgcggc cgccagcgtc tgctcgaaca ccgcggcgtc
     5221 gaccacctgc ttgtccggca ggtgtccgca ccgcttcggg aacacctggt cttccagctg
     5281 gatcgcggcc acccctgccg cgtcgtacga aacgacggtg cgcgccacgt tcatgggtgc
     5341 gccatagcca gtgtcggcgt cggcgatcag cgggatgtcg cccagtgcgc cgacgatcat
     5401 ccggacccgg tcggccattt cggtcgccgt gaccagcccg atgtcgggca atccgaagcc
     5461 cgacgccgcc acgccggccc cggtcatgta cgccgcgacg tggccggtcc gtttggtcag
     5521 gtgggcggag atgccgtcga agacccctgg cgcgacgatc agttcgcgcg tgtccagcag
     5581 ctctttcagc cgccggcggg caggtgtggt cgccatgaga gtccccttag tccagtgcgc
     5641 tcgcgacggg gtcagcgagc aggtcgatca agtggtcgac gtcgtcaagg tcctcgagac
     5701 agacgaccgc ccgctcgatc gcttcggtgc gctcccggct ggttacccgg tcggcgagcg
     5761 cgtggaattt ggcgacgagt tcgtcgttgg tgaccgggtc ggtgggcgcg ccgtgcggca
     5821 cgtcgacgcg ggcgtggtgc acggcgccgt catgcgtggt cagcgcgacg tcggtgcgga
     5881 acttcgtggt gatgtcggcg tgggccagcg tttcgtcgag gtgcaccgac gtagccccga
     5941 gcaatgacca gatgtcgtcg gagtccaggc gaccgggggt gaactgatcg ggcagcacgt
     6001 tgccgtcgag cagcgcggcg gcggtcgcgt acccgatgtt catctgcgcc ccgatcggcg
     6061 tcaacggcct ctccggcagc caccaaccgt gcttgtagat agtttcacca acggtgatgt
     6121 cgaccttcga gatgtcctcg ggggcaaccg cattacgcaa ctgtcgggcc gcgtcgatcg
     6181 ctccgtgcag accgcccatc gcggcgtacg acttgaccat gatgatggtg gtctcccagc
     6241 gttggccgag ctgcccggtc agcaacgacg cgtcgggatc gtggccttcg ccgaaaacgc
     6301 tgagatatcc gccatattcg cgctcgaaca cccgcttgat accggtgtag ccggccgcgg
     6361 caagcccggc cgcgtagaac ccgttgcgcg ccgccaggcc gtggtgcatg cgcttgctca
     6421 tcgcctcgta ctgcgcggcc atcagtcccg ccgactgggt gccggccaag ccgagcgcgt
     6481 cctccagctg acccggcggc agaccccgca gcttgcccga cgccatggcc gccgagtggg
     6541 tgccaaagac gggtcctgaa tgccagcccc ggtccagcat ctgggtgccg tgcagcgtgt
     6601 acccgacccg cgggcccact tcgaagccgg cgatcgcccc gagcaggaat tcggcaccgg
     6661 ttgttgtttc cggccgcgtc gttgcggtgg acagcagcgc cggaatgagc agcgagcagc
     6721 tgtgcagcgg tgccaggggg tggaagtcgt cgagctcgaa cccctggatg aacgtgccgt
     6781 tgagcacggc agccgccggc gcgctggtgg tatgtccggt gccgattacg acggtgccgc
     6841 cggagccttc caatccgagt acggcctccg tggcgattcg cgaccacggc aatcgggccc
     6901 caaccagggc gcaacccagg ccgtcgagga ggaggtgttt tgcccgttcg gtgacgctgt
     6961 gcgggacctg atcgagtgtg aggtcggcga cccagccggc cagccttccg gtcgggccgt
     7021 tcggatcggt ggcaggttgc cggtgcacgg cggtcattgg agctcccctc cttgcttcat
     7081 gcttggcttg ttgttataaa catatgacaa gtatgagcac catgcaagag ggcggtgtgg
     7141 aagcattgac caccgtgcac aagaccagtg gcaaggcgtc gccggtgtcc actgatgccg
     7201 gcgtgccgtt gcaccggcaa cttttcttgg tgctgcacga cgagatcgac cggggtgtca
     7261 tcgcccccgg cgagccgttg ccgagcgaac agacgctgtg cgatcaattc ggggtgtctc
     7321 gcatcacagt gcgccgcgcc ctggcggatc tggccgaaca gggatacatc gagcgcaggc
     7381 agggcgtcgg ctcgttcgtg cgcgagcacg gtccggccga cgcgtcagac gctttgggtg
     7441 ggggcggctc gtacatggcg gggctgcgac agagccaatt cgaaaccgag gtcgacgtgg
     7501 ttgaactcgg cacgcggcgg cccccgcggg cgatcgccga ggcgctccgg agttccggtg
     7561 agctgctgca catcgtgcgg gtgcggcggc aacgcaggac cgccgagccg ttgatggtga
     7621 ccgacgcgtg gctgccggcg gacctggccg gcacgttgac cgagtccgcg ctacgacggg
     7681 cgccgctcta ccagctgctg tcggatgccg ggatcgcggt gggccggatg cggcacgaaa
     7741 tcaccgccga gatcgccggc ccgcgcaacg cgcacctgct ggacacggcg atcggcgccg
     7801 cattgttgcg cgtcaaccgc ctggctttcg tggctgacgc gccgcatcat cacctgtcgg
     7861 tgctgctatc gccgaaccgc agtcgtgtgc tgctcagcca gaccggcgac gaactggaaa
     7921 tggccgatgg tttgaccatc gcccacgacg tggccaggcc acgcgctaac gaatgagtcc
     7981 gacaccgggt acgaatcgac ccacgctggc ggcgtaagcg cgaaaggcgt cgccgtgtgt
     8041 ccgcaacagg tacggctctt cgacacgacg gacctggagt tggatcatgg cgacgagcag
     8101 gataaagctg gcgcaggcaa caagattcgg cgtcaccagg gcgatcccga cgccgaaggt
     8161 gaacatggcg gtgtagatgg gattgcgtat ctggccgaag acgccggttt gcactagcgt
     8221 ggtcacctct ttctcgtcaa ctccgatccg ccaggaatca cccatctcga gttgcgcata
     8281 aaccgttgcc acgatgccgg ccgttgcgat gacgatgccg acggcctgga cccacaccac
     8341 gctgagcgcc ttgacgggtt gtatgacctt ggtctcctgc aggatcgacg cgctcaccgc
     8401 cacgatcagc gcgatgacaa agccgacccc ggcgacgcct tccgtaaaac caccggcgct
     8461 gacgccacga aagccggtcg agccggtgcg ccgatactgc agccaactgc gccaacccaa
     8521 tccgagcacg ccgaacaccg cgaacagcac cagggccatg tcggcggcgg ctgtcgctgg
     8581 catcagcagg ctcccacgac cgcagtcatg gccagcgaca gtacatcaaa ggctgtggtc
     8641 atgaaaggcc gtcccgcagc gcgttgtcga tcgctgcggc acccggtgcg cagtcaccgg
     8701 cgcccggcgt caacgtcgcc agcgcacccg ctgcgcacgc acgccgcaac gcggccagcc
     8761 gctcggcggg cgaacctggg ttgggtggcc agttcgcggc cagcacaccg gcgaacacgt
     8821 cgccggcgcc ggtcgtgtcc acggcgtcga ccacaggggc cggtaccgcg aattcgccgt
     8881 cggtgccgat gtagcgggcg ccgcgcgcgc ccagtgtgat caccaagtgg gctggccgcc
     8941 agggccaccc gtcggcttcg gtttcattgg cgatcaccac gtcggcgacg gccgccaatt
     9001 cggccaggga gccttggtcc gggccggccg gtgaggcgtt gacgatgacg accgccccgg
     9061 ccgaacgcgc ttcgcgggcc gccgccaccg ccgtcggcac cggaatctcc aactgggtca
     9121 acagcacatc cgatgcgcca atgacctcgc gcgccgccgc ggcggacaac gtcagccgcc
     9181 cgttggcgcc cggcgcgacc acgatcgtgt tctcgccgcc ggcatcgacc acgatgatcg
     9241 ccgccccgct cggtccggtc aatgcgacgg tcccgtccaa gccaacgccg ttggcccgca
     9301 ggtgagcccg caactgatcg gcggcgggat cgtcgccaac ggcgccgacg aactgcacct
     9361 gggcgccggc gcgggccgct gcgaccgcct gattgccgcc tttgcccccg ggtgcagagg
     9421 cccatgacga cgccaacacc gtctcgccgg gtcggggaag ggcggcgacg tcgaacgtcg
     9481 aatccatgtt cacgctgccc accacgcaga cccgcgccgc cattccgccg acgttagtcg
     9541 gttcgggcta cgcaacgcag gagggtggtt cttcggcaca gaatcacttg cgcctcaggg
     9601 gtttcagtgg tttcagcgca gcgcatgttg tcggcgatcc ggcgtggtat tcgaactcgg
     9661 gttcgagtag ccgggaagtg atccactggg attttgggct gccgaggcga cccgacagaa
     9721 cgcgtcagga tgactgtttg atctgtatct ggtcggtttg tgtgtatccc ggcggtcggc
     9781 cgcacgccgg cacgatgtag acgtgttgaa ctgtgccttc aagggtatct ggatcccacg
     9841 tcatgtggga gttcgtggcg tattggatgt aactgccgtc cgagcaattt acgttgttga
     9901 gcgtgtccag tacccattgg ccatcgacca accgcgcttg gctacccccg gccccggctt
     9961 ttatgtagat gcatccgtca ccgcagggac tgacggtcca cgaggtggta atggttcggc
    10021 ccgccggcgt tgtcgaggtc tcggtgtaat tgccgttcat cgtcggtacg tcagcccacg
    10081 ctgtgctagc tgtaccgaca gctaaagcgg cgaacatagc ggttgcggcg aagccccgtg
    10141 cgacggccat gctctcgttc cttccattga gctgatcaat gcgtcgagca ctgttgatgg
    10201 tgtatcacag gttcagggtt tgtcgcgatt tggcatactt ggccattccc gggcagcgtt
    10261 ttctgacaac ccaggatgac tgttggcgtc ggtaaagctg ttccagcaca acgggttaac
    10321 tgcgtccatc gtcgtgacca ccactctcag agaccttgaa gccgctaccg ggaaagggct
    10381 caccggcggg ggcagtctgc tggcacacca aacgttttgc gtcgcccggg cagcgaattg
    10441 cgctgtacgc cggtgctgct gggccccgtg aaccgccgac tggctatccc gtctcctccg
    10501 atgggggaat gtcgttggcg tcccttgact ggttttccgc gtcgatccga aggtgtgcgg
    10561 tcagcactaa gttgtcgtag gccgaccgag gttggagcgt ctcgccgcga ctgagttcga
    10621 ggaagtcggt ggcgatttgc tcggccaagg tccgcagttt gacgttcgcc tcctgggatc
    10681 gccacttgag cagttcgaag gcggttagtt catcgattcc gtagaccagc atcaacatgc
    10741 ccttggtctg ttcgattccg gaccggctct cggtgattct ggcgacggct gcggtcactt
    10801 cgtcttggcg tgcccgttcg gaaggtgtga cgtcgatgta gaagccgtgc gtgccgatca
    10861 cgctctcgtc gtcgtcgcgc agcaggtcgc caacgaccac gacgtgatgt acccggcctt
    10921 ccacgtcgat gatgcggtgt cgactgctga acgcttgacg actttggcgg atcaactcga
    10981 gggtgtgcgc gatctgctgg tagtcatcgg gatgcttgtg agccagcacc atttcggtgg
    11041 tcggagccac tgaacccggc agatagccgt gcatcttctc cacttggggc gaccactccc
    11101 aacggtcgtc gtcgaagtag aagcggaacc agcccacccg gtgcggtccc gtatcggcca
    11161 atgcctcccc gacatctacc ttgctgtcac tgccgctgga ctgctcaacg gcgaaccaac
    11221 tctcgttttc tggatgtctt tgcatcgcat accccttgtg cggaataccc tctgctgatt
    11281 tgtgtgctcg gcaacctctg gaacatcaga tcctggcggc gaagacatct gcgcgacgag
    11341 ggtcttccgc ccgggggtgt acggagcaat aaatttttta ctactagctg gcacccaggt
    11401 caacccccat tttgggcacg aaaaatggcc gtcgtgtggc aacgcgaagg accgcgacgc
    11461 gatccgaccg tcgactactc gaacccggcg gctggaccac tcgcaaacgc aaagacggca
    11521 gcaccgaatc gatctcccca accactacga ttgcgcccaa cccaggacga acacgttcca
    11581 tcactggcaa aaagtcattg cggaagaagg ggacaccagc cgtaagtccc gtgccgctgg
    11641 tgctgctcac atctgtggca atcgacaata tcgtgatgtt cgcgcgttag tctgctgcgt
    11701 gatcggtgct gaatcccatc gaagggggag gaaagtcgat tgacatctag cgagctgaag
    11761 gacctaaaag acggagccga accggcctcc acagccgacc cggaaaccgc cccggaatcc
    11821 gcccccgctg aaaagtcccc gaagcgtacg cgccgtcctc tggtgtggac gcgtttccgg
    11881 ttcggcatcc aatcgaagat cctggttgcg atgctgttgt cgagcatcct gggcgtgtcg
    11941 gtgatcggcc tcatcggggc tttgtccggc cgcagcgcgt tgcggcaagt cgagtccgag
    12001 cggttgatcg agttgcgaga gacgtcgaag cgccaggtcg aggcgctctt cagggaggtg
    12061 accaattcgc tgatcgtcca cagcggcggg ttcagagtca ccgaggcaac ggcggagctc
    12121 accgcggtgt ttaaccaatt ggccaacgcc acgatcaccc ctgcccaaca acaggcgctc
    12181 gtcacctact acgacaacga catga
//
LOCUS       DASSGQ010000339        11798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1422732,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000339 DASSGQ010000000
VERSION     DASSGQ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11798
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422732"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..928)
                     /locus_tag="VFI55_14860"
     CDS             complement(26..928)
                     /locus_tag="VFI55_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7075343.1"
                     /translation="MSIRSLGYLRIEATDMAAWREYGLKVLGMIEGRGGAEGALYLRM
                     DDFPARLVIVPGEHDRLSEAGWECANAEGLQEIRHRLDVEGTPYKEATAAELADRRVD
                     EMIRFSDPSGNRLEVFHGAALEHRRVVSPYGHKFVTGEQGLGHVVLSTRDDDEALHFY
                     RDVLGFRLRDSMRLPPQLVGRPADGAPAWLRFFGCNPRHHSLAFMPAPTPSGIVHLMV
                     EVEGADDVGLCLDRALRRKVPMSATLGRHVNDLMLSFYMKTPGGFDIEFGCEGRKVDD
                     DDWIARESTAVSLWGHDFTVGFRG"
     gene            complement(925..1020)
                     /locus_tag="VFI55_14865"
                     /pseudo
     CDS             complement(925..1020)
                     /locus_tag="VFI55_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(1015..1158)
                     /locus_tag="VFI55_14870"
                     /pseudo
     CDS             complement(1015..1158)
                     /locus_tag="VFI55_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(1176..2360)
                     /locus_tag="VFI55_14875"
     CDS             complement(1176..2360)
                     /locus_tag="VFI55_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent monooxygenase"
                     /protein_id="HET7075344.1"
                     /translation="MTSIQQRDAQSVLTGIDDLLPQIRGRAQATEDLRRLPDETVQEL
                     EDVGFFRLLQPEQWGGLQCDPGLFYEATRRLASACGSTGWVSSIVGVHNWHLALFDQR
                     AQEEVWGEDSKTRISSSYAPMGAGVVTDGGYLVNGSWNWSSGCDHASWTFVGGPVIKD
                     GRPVDFGSFLIPRPQYRIDDVWHVVGLRGTGSNTLVVKDVFVPRHRFLSYREMNDHTA
                     GGLQNNTAPVYKMPWGTMHPTTISAPIVGMAYGAYAAHVEHQGRRVRAAFAGEKAKDD
                     PFAKVRIAEAASDIDAAWRQLIGNVADEYALLAAGKEIPFDLRARARRDQVRATGRSI
                     ASIDRLFEASGATALSNDAPIQRFWRDAHAGRVHAANDPERAYVLFGNNEFGLPPGDT
                     MV"
     gene            2517..3608
                     /locus_tag="VFI55_14880"
     CDS             2517..3608
                     /locus_tag="VFI55_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HET7075345.1"
                     /translation="MTEAIADEPLGDHVLELQIAEVVAETDDARSLVFAVPDAPGDPD
                     IPLKRLRYAPGQFLTLRIPSDRTGSVARCYSLCSSPFTDDALTVTVKRTADGYASNWL
                     CDHAHPGMRIHVLAPSGNFVPKTLDGDFLLLAAGSGITPIMSICKSALSEGGGEVTLL
                     YANRDDRSVIFADALRELSGKYPDRLTVVHWLESVQGLPSATALAMLAAPYADRPVYI
                     CGPGPFMDASREALAALKVPAPQVHIEVFKSLDSDPFAAIKIEDTDEGADHGAAPATA
                     IVELDGETHTVSWPRNAKLLDVLLANGLDAPFSCREGHCGACACTLRSGKVNMEVNDV
                     LVQQDLDEGLILACQSHPESDSVEVTYDE"
     gene            3671..4204
                     /locus_tag="VFI55_14885"
     CDS             3671..4204
                     /locus_tag="VFI55_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075346.1"
                     /translation="MKRLMTGFAVVGSVLTLLPTPLCAAASNTATTLFPLDGPNQLET
                     HTFLNCFQGDGHCDFTAGADMRTPDGVTGFPPSLWARQTTEIRSSNRMAYLDAHATGQ
                     YERVMKSMGSDEITSVYFGEGPPDKYQTTGRIDSTDWSTGQPKTDVNVIVCTHIQVVY
                     TGVNITSPATCAQTTFS"
     gene            complement(4287..5162)
                     /locus_tag="VFI55_14890"
     CDS             complement(4287..5162)
                     /locus_tag="VFI55_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013830923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075347.1"
                     /translation="MRVVFIGSEAIASADVTRHELQRWYAPIYPGVYAARGQQLSLRR
                     RTEGAWLWSRRRAIVAGVAASALHGAQWIDTDIPIELVWSNTRPPQGIVAREQLLADD
                     ETTRVAGLPVTTPVRTAYDLGRYLARGQAVARLDALMRATPFSAKDVLLLAERYPAAR
                     GVRRLRSVLHLVDGGAASPKETWLRLLLIDAGLPTLTTQIPVVDGYRALAMLDMGWPE
                     FGVGVEYEGDHHRSDRRQYVKDQWRLRRLAEMGWIIIRVIAEDKPEDVIERVRRALLA
                     RGWRPKRAAVVLSAG"
     gene            complement(5266..7431)
                     /locus_tag="VFI55_14895"
     CDS             complement(5266..7431)
                     /locus_tag="VFI55_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET7075348.1"
                     /translation="MVATVADEQFAARALVRDWARNPNSGPGGTTAIRAMEQGHIDAW
                     RPVFAGLADLGLFGVAIPEYLGGAGGTIEDLCAMVEEAAKALVAGPVATTALATVVLS
                     GSVDDQKLLAALASGERFAGVALEASEGSLQFDERASRATGSVPWVLGAAEGAVLIVP
                     ADGRSLLIDTGSDGVRIEPLEAADFSRPLARVVLTSAPATVLTGSVWASPRRVEELAA
                     TLLAAEAAGVTRWSLDTAVAYAKVREQFGKPIGSFQAVKHLCAEMLCRAEQAEVAAAD
                     AARAAADSDQSQFSLAAAVAASIGIAAAKANVKDCIQVLGGIGCTWEHDAHLYLRRAH
                     GIGRFLGGAERWLRRSTALTQAGVRRRLGIDLSEVEGMRPEISGAVAGVAALPEEQRR
                     TALAEAGLLAPHWPVPYGRGASPAEQLLIDQEMSAAGVVRPDLVIGWWAVPTILQHGT
                     GEQVERFVPATMRGEFLWCQLFSEPGAGSDLASLRTKAVRAPASAGGWLLTGQKVWTS
                     AAHKARWGVCLARTDPDAPKHQGITYFLVDMTSPGIEIRPLREITGDSLFNEVFLDDV
                     FVPDAMVVGAVNDGWRLARTTLANERVAMATGTALGNPMEELLKLLARIDLDVAQQDR
                     LGRLIVTAQTGALLDQRIAQLAVGGEDPGAQSSVRKLIGVRYRQALAEYMMDVSDGGG
                     LVQNLAVHDFLNTRCLTIAGGTEQILLTVAAERLLGLPR"
     gene            7770..8369
                     /gene="kstR"
                     /locus_tag="VFI55_14900"
     CDS             7770..8369
                     /gene="kstR"
                     /locus_tag="VFI55_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol catabolism transcriptional regulator
                     KstR"
                     /protein_id="HET7075349.1"
                     /translation="MAVLAESELGSEAQRERRKRILDATMAIASKGGYEAVQMRAVAD
                     RADVAVGTLYRYFPSKVHLLVSALGREFGRIDAKTDRSAVTGATPFQRLNFMVGKLNR
                     AMQRNPLLTEAMTRAYVFADASAASEVDQVEKLIDSMFARAMADGEPTEDQYHIARVI
                     SDVWLSNLLAWLTRRASATDVSKRLDLAVRLLIGDSEKT"
     gene            complement(8391..9479)
                     /locus_tag="VFI55_14905"
     CDS             complement(8391..9479)
                     /locus_tag="VFI55_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7075350.1"
                     /translation="MSPIPRRHATLASLADELNVSRTTISNAFNRPDQLSPELRERVL
                     ATAKRLGYAGPDPLARSLRTRKAGAVGLLMAEPLTYFFSDPAARDFVAGVAQSCEELG
                     QGLLLVAVGPGRSTEDGTAAVLSAGVDGFVVYSACDDDPYLQVVLQRRLPVVIVDQPK
                     GLSGVSRVGIDDRAAMREIADYVLGLGHREIGLLTMRLGRDRRQGLVDGDRLQSPAFD
                     VQRERIIGVWEAMKAAGVHPESLTVVESYENLPTSGGAAAKVALEANPRITALMCTAD
                     VLALSAMDYLRAHGIYVPGQMSVTGFDGVPEAISRGLTTVAQPSLQKGQRAGDLLLKP
                     PRTGLPGLPVVELLDTELIRGRTAGPPA"
     gene            9661..10371
                     /locus_tag="VFI55_14910"
     CDS             9661..10371
                     /locus_tag="VFI55_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015303958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HET7075351.1"
                     /translation="MRQQLAALVLGGVSALAIGCGTTVNGRAVAADTSGPRSQNPVAV
                     SALDGLLLDAGQINAALGATSMNVWFAANAMWDWGSGVNDKSCLAIDGPAQDQVYVNS
                     GWTAMRGQRLDDSADDSKKRDHYAIQAVIAYPSAREANAFYDSSEQSWSACSNRRFSD
                     ATPGQPDTVWTVSGLTTDNGTLSTSQVQEGGDGWACQRALTVRNNVAIDIVACAFNQS
                     GGPAVDVANQIAAKVAKQ"
     gene            10375..11112
                     /locus_tag="VFI55_14915"
     CDS             10375..11112
                     /locus_tag="VFI55_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HET7075352.1"
                     /translation="MRRLLCAVRTATCAVAMAATWCTGCDRTVEGAAFAARTPASAAV
                     TTTVNPSPSPLPVAGLESLLLTADQLSAVVGGANMALIGTATSTSDASKLIDDRTCLG
                     LGSVGDVVSYSNSGYVAMRGNQFSTPSAVQADVTQLVTSFTTAADAQALLQRTRQDWQ
                     GCAGRRYGFHSSNGNHSYFDTGDLRGSSSRIEVSMSQEEDARWGCSHALAVGNNIVAE
                     ARVCQLGKDTAVAVNGLLDQIVAKIPQ"
     gene            11122..>11798
                     /locus_tag="VFI55_14920"
     CDS             11122..>11798
                     /locus_tag="VFI55_14920"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HET7075353.1"
                     /translation="MAGIDLNSDLGESFGVWRLGDDDAMLGIVTSANVACGFHAGDPA
                     GLLRVCRSAAERGVRIGAQVSYRDLAGFGRRFIDVATEDLVADVVYQIGALQAIAHTS
                     GSAVSYVKPHGALYNTIVTNREQGAAVAEAVRMVDPTLPVLGMAGSAFFDEATRRGLR
                     TVAEAFADRAYRPDGRLVSRREPGAVLHDPAAIADRVVSMVTSGHVAATDGSQVAVSV
                     ESVCVHG"
BASE COUNT         2032 a         4065 c         3766 g         1935 t
ORIGIN      
        1 cgcggatcga tcggtgccga catggtcagc cgcgaaagcc gaccgtgaag tcatggcccc
       61 acaggctcac cgcggtgctc tcccgggcga tccagtcgtc gtcatcgact ttcctaccct
      121 cgcagccgaa ttcgatgtcg aacccgccgg gtgttttcat gtagaacgac aacatcaggt
      181 cgttgacatg ccggcccagc gtggccgaca tcggcacctt gcggcgcaac gcccggtcca
      241 ggcacaggcc cacgtcgtcg gcgccttcga cctcgaccat caggtgcacg atgccgctgg
      301 gtgtcggcgc cggcatgaag gccaagctgt ggtgacgcgg gttgcagccg aaaaagcgca
      361 gccaggccgg cgctccgtcg gcgggccggc caaccagctg cggcggcaag cgcatcgagt
      421 cgcggagcct gaagcccagc acatcgcgat agaagtgcaa cgcctcgtcg tcgtctcggg
      481 tcgacaacac cacgtggccc agcccctgtt cgccggtgac gaacttgtgg ccgtaggggc
      541 tgaccacccg gcggtgttcc aaagcggcgc cgtggaagac ctccaggcgg ttgccagacg
      601 ggtcggagaa tcggatcatc tcgtccaccc ggcgatccgc caactcggcc gcggtggcct
      661 ccttgtacgg cgtaccctcc acatcgagcc gatgccggat ttcttgcagg ccctcggcat
      721 tcgcgcattc ccaaccggct tccgagagcc gatcgtgctc accgggcacg atcaccagcc
      781 gggcagggaa atcgtccatc cgcagataga gcgcgccctc ggcaccgccc ctgccttcga
      841 tcatgccgag caccttcagg ccgtactcac gccacgccgc catatcggtg gcctcgatgc
      901 gcagatagcc cagcgaccga atgctcatcg cgcacctccc aggaaatcaa tcgtcagctg
      961 gttgaattcg tcgaactttt ccacctgcac ccagtgccca cattgcccga agacgtgcag
     1021 cagcaccacc gtctggtcgt tgccgacgcc cgcttcgtgg tagtgcagtt tcagggggcc
     1081 gcccagatct gggccgttca cttcggcgaa gcgcgaggtg gactcgaacg tcaattcctc
     1141 gatctggctg acggtcatgg ctcaggcttt gccgttcaaa ccatcgtgtc gccgggcggc
     1201 aacccgaact cgttgttccc aaagaggacg taggcgcgct cggggtcgtt ggcggcgtgc
     1261 acccggccgg cgtgcgcgtc gcgccagaac cgttgaatcg gggcgtcatt ggacaacgcc
     1321 gtggcgccgg acgcctcgaa cagccggtcg atcgaggcga tcgaccgccc ggtggcgcgc
     1381 acctggtcac gtcgtgcgcg ggcacgcagg tcgaacggaa tctccttgcc cgcggccaac
     1441 aatgcgtact cgtcggcgac attgccgatc aactgacgcc acgcagcgtc gatgtcgctg
     1501 gcggcttcgg cgatgcgaac cttggcgaac gggtcgtcct tggccttttc gccggcgaac
     1561 gccgcgcgca cccgcctgcc ctggtgctcc acgtgcgcag cgtatgcgcc ataggccatg
     1621 ccgacgatcg gtgccgaaat ggtggtggga tgcattgtgc cccacggcat tttgtatacc
     1681 ggcgcggtgt tgttctgcag ccctccggca gtgtggtcgt tcatttcccg gtaggacaag
     1741 aaccggtgcc ggggcacgaa gacgtccttg accactagcg tgttgctgcc cgtcccgcgc
     1801 agcccgacca cgtgccacac gtcgtcgatc cggtactggg ggcgcgggat caaaaagcta
     1861 ccgaagtcga ccggacggcc gtccttgatg accgggccgc ctacgaacgt ccagctggcg
     1921 tggtcgcagc ccgacgacca gttccacgat ccgttgacca ggtagccacc gtcggtcaca
     1981 acgcccgcgc ccatcggggc gtacgacgac gagatccggg tcttcgagtc ctcgccccag
     2041 acttcctcct gggcgcgctg gtcgaacagc gccaggtgcc agttgtgcac tccgacgatc
     2101 gagctcaccc agccggtcga gccgcacgcg ctcgccagcc gccgcgtcgc ctcgtagaag
     2161 agccccggat cgcactgcaa cccgccccac tgctcgggct gcaagagcct gaagaagccg
     2221 acgtcctcga gctcttgaac cgtctcgtcg ggcagccggc gcagatcctc cgtcgcttga
     2281 gcgcgtccgc gaatctgcgg aagcagatca tcgatccccg tcagaacaga ctgcgcgtca
     2341 cgctgttgaa tggacgtcac tgaggtttgc ctcccgggcc agactgatac agcagactac
     2401 accagagact agaacacgtt ccgatttgtg tcgagcacgc tattcctgcg cctggtagcg
     2461 atgtgaatcg ggttttctgt aacatgttct agttgcgagg agagggaagg cgggtcttga
     2521 cggaagcaat tgccgacgag ccactcggcg accacgtcct tgaactgcag atcgccgagg
     2581 tcgtcgccga aaccgacgac gcgcgatcac tggtgttcgc ggtgccggac gcgcccggcg
     2641 atccggacat cccgctgaag cgactgcgct acgcgcccgg ccagttcctg acgttgcgca
     2701 tacccagtga ccgcaccggc tcggtggccc gctgctactc gttgtgcagc tcgccgttca
     2761 ccgacgatgc gctcaccgtc acggtcaagc gcaccgcgga cggctacgcg tccaactggc
     2821 tgtgcgacca cgcacacccc ggcatgcgga tccacgtgct ggccccgtcg ggcaatttcg
     2881 tacccaagac gctggacggc gacttcctct tgctggcggc cggtagcggg atcaccccga
     2941 tcatgtcgat ctgcaagtcg gcgctttccg agggcggggg ggaggtgacg ctgctctacg
     3001 ccaaccgcga cgaccgctcg gtgatcttcg ccgacgcgct gcgcgagttg tccggcaagt
     3061 atcccgaccg gctcacagtg gtgcattggc tggagtcggt gcagggcttg cccagcgcga
     3121 ccgcgctggc gatgcttgct gctccctatg cagatcgccc cgtatacatt tgcgggccag
     3181 gcccattcat ggacgcatca cgcgaagcgc ttgcggcgct gaaagtgcct gcgccgcaag
     3241 tgcatatcga ggtgttcaag tcgctggact cagatccgtt cgcggcgatc aagattgagg
     3301 ataccgacga aggtgctgac catggggcgg cgccggccac cgcgattgtc gagctggacg
     3361 gcgagaccca caccgtgtcg tggccgcgca acgccaagct gctcgacgtg ttgctggcca
     3421 acggcctcga cgcgccgttc tcctgccggg agggccactg cggcgcgtgc gcctgcacgt
     3481 tgcgcagcgg caaggtcaac atggaggtca acgacgtgct ggtacagcag gatctggacg
     3541 aggggctgat tctggcctgt caatcccatc ctgaatctga ttcggtagaa gtgacctacg
     3601 acgaatagtc gcggagttaa gttctgacct ggggtcttct cgacggcgga gtaacgggaa
     3661 ggggagcagg atgaagcggc tgatgacggg cttcgcggtc gtcggatcgg tgctgacgtt
     3721 gctgcccacc ccgctctgcg cggcggccag caacaccgcc accaccctgt tcccgctcga
     3781 cggccccaac cagctcgaga cacacacctt cctcaattgt tttcaaggcg acggccactg
     3841 cgacttcacc gccggcgccg acatgcgcac ccccgacggg gtgaccggat tcccgcccag
     3901 cctgtgggcc cgccagacca ccgagatccg gtcatcgaac cggatggcct acctggacgc
     3961 ccacgccacc ggccagtacg aaagggtgat gaagtcaatg ggatccgacg agatcaccag
     4021 cgtctacttc ggtgagggtc cgccggacaa gtatcagacg acgggccgca tcgactcgac
     4081 cgactggtcc accgggcagc ccaaaaccga cgtcaacgtg atcgtctgca cccacattca
     4141 ggtggtctat accggagtca acatcacctc gcccgccacc tgcgcgcaaa ccaccttttc
     4201 gtgatcgccg agcatgtata tctgcagaca aagtgcgcgt aaaggcctgc aggattaaag
     4261 gctcgacgaa acgagccgac tggcagttac ccggccgata ggactacggc tgcccgcttg
     4321 ggtcgccaac ctctagcgag cagcgcccgc ctgacgcgct cgatgacatc ttcgggcttg
     4381 tcctcggcga tcacgcgaat aatgatccag cccatttcgg cgagccttcg tagtcgccac
     4441 tggtccttta cgtattgacg ccgatcgctg cggtggtggt cgccttcata ctcgacaccc
     4501 acgccgaact ccggccagcc catatcgagc atggccaggg cgcggtagcc gtcgaccacc
     4561 ggtatctggg tggtcagggt cggcaagccc gcgtcgatca gcaggagccg cagccaggtc
     4621 tcctttggcg aggcggcacc gccgtcgaca aggtgcaata ccgaccgcag ccgtcgtaca
     4681 ccgcgggcgg cggggtatcg ctcagcaagc agcagcacgt ccttcgcaga aaacggcgtc
     4741 gcccgcatca acgcgtcgag gcgcgcgacc gcctgcccgc gcgccagata ccggcccagg
     4801 tcgtacgcgg ttcgcacggg agtggtgact ggcaatcccg cgacgcgggt ggtttcgtcg
     4861 tcggccagca gctgctcacg cgcgactatc ccctgcggtg gccgcgtgtt cgaccagacc
     4921 aactcgatgg ggatatcggt gtcaatccac tgagcgccat gcaatgccga agccgcgaca
     4981 cctgccacga tcgccctgcg ccgcgaccac agccatgcgc cttcagtcct gcgccgcagc
     5041 gaaagctgtt gaccccgtgc ggcatacact cccgggtaga tcggcgcata ccaacgttgc
     5101 agttcgtgtc gcgtcacatc tgcgcttgcg atcgcttcac tgccgataaa caccacccgc
     5161 atgcccgcat agtggcatca gcgaccgaca cgtcgccgat cctgcaaatc tgcagacaaa
     5221 gtccgcgtaa aggcctgcag aattacagac tcgacgcaaa gcccgctagc ggggcagccc
     5281 gaggagccgt tcggcggcga cggtgagcag gatctgctcg gtaccgccgg cgatggtgag
     5341 gcatcgggtg ttcaggaaat cgtgcaccgc gagattctgg acaaggccgc cgccgtcgga
     5401 cacgtccatc atgtattcgg ccagcgcctg ccggtagcgg accccgatga gcttgcgcac
     5461 actggattgc gccccggggt cctcaccacc gacggccaac tgggcgatgc gctgatccag
     5521 cagcgcaccg gtctgcgcag tgacgatcag gcgtcccaac cggtcctgct gggcaacgtc
     5581 gaggtcgatc cgtgccaata gcttcagcag ctcttccatc gggttaccga gggcggtccc
     5641 ggtggccatc gcgacccgct cgttcgccag tgtggtgcgg gccagccgcc agccgtcgtt
     5701 caccgcgccg accaccatcg cgtcggggac gaacacgtcg tccagaaaca cctcgttgaa
     5761 caacgagtcg ccggtgatct cacgcaacgg ccggatttcg atgccgggcg aggtcatgtc
     5821 caccagaaag taggtgatgc cctgatgttt tggagcgtcc ggatcggtgc gcgccaggca
     5881 cacgccccac cgcgccttgt gcgccgcgga tgtccacacc ttctgcccag tgaggagcca
     5941 accgcctgct gaggctggtg cccgcaccgc cttggtccgc agcgacgcca ggtccgagcc
     6001 ggctcccggc tcggaaaaca gctggcacca aaggaattcg ccacgcatcg tggccggcac
     6061 gaagcgctcg acctgttcgc ccgtaccgtg ctgaaggatg gtcggcaccg cccaccagcc
     6121 gatcaccaag tccgggcgca ccacgccggc cgccgacatc tcctgatcga tcagcaactg
     6181 ctcggccggt gacgcgccgc gcccgtacgg caccggccag tgcggtgcca gcagccccgc
     6241 ttcggccaac gccgtccggc gttgttcctc gggcagcgcc gcgaccccgg cgacggcccc
     6301 ggagatctcc ggtcgcatgc cctccacctc ggacaggtcg atacccaggc ggcggcgcac
     6361 acccgcctgg gtcagcgccg tgcttcggcg cagccagcgc tcggcgccgc ccaggaaccg
     6421 cccgatgccg tgcgcccgcc gcagatacag atgcgcgtcg tgctcccagg tgcagccgat
     6481 gccgccgagc acctgaatgc agtctttgac gttggctttc gcggcggcga taccgatgct
     6541 cgcggccaca gccgccgcga gagaaaactg gctttggtcg gaatccgccg cggcgcgggc
     6601 cgcatcggcg gcggccacct cggcctgctc ggcgcggcag agcatctccg cacacaagtg
     6661 cttgacggcc tggaagctgc cgatcggctt gccgaactgc tcgcgcacct tagcgtaggc
     6721 gaccgcggtg tccaacgacc accgggtcac gccggccgcc tcggccgcca gcagggtcgc
     6781 ggccaattcc tcgacccgcc gcggcgatgc ccaaaccgac ccggtgagca cggtcgccgg
     6841 cgccgacgtc aggaccaccc tggccagcgg ccgggaaaag tcggctgcct ccaacggctc
     6901 gatccgcacc ccgtcgctgc cggtgtcgat cagcagtgac ctgccgtcgg caggcacgat
     6961 caggacggcg ccctccgcgg cgcccaacac ccacggcacg ctgcccgtcg cccgcgacgc
     7021 ccgttcgtca aactgcagag agccttctga ggcttccagc gccactccgg cgaatcgttc
     7081 cccggaggcc agcgccgcca gcagcttttg gtcgtcgacg ctcccggaga ggaccaccgt
     7141 ggccagcgcc gtggtcgcaa ctggcccggc taccagcgct ttagcggctt cctcgaccat
     7201 cgcgcacaga tcctcgatgg tgccgcccgc cccgccgagg tactcgggga tcgcaactcc
     7261 gaacaaccca aggtcggcca gaccggcaaa taccggccgc cacgcgtcga tgtggccttg
     7321 ctccatcgcg cggatcgccg tcgtcccacc cggccccgag ttcggattgc gcgcccagtc
     7381 gcggaccagt gcacgcgcgg cgaactgttc gtcggcgacg gtcgctacca cgtgtgactc
     7441 cttcgcgtcc tgattcgatg tgaccacggg gcgctcgacc ccggttctcc gattgcccgc
     7501 attgcccgcc tctgactcgg gttgctttgg caacccgcat cgtcgacaga ctagaacgtg
     7561 ttctaatagt gccagcgatc gaacgtcaag tcgaacggct ttgcagcagt acatatcgtc
     7621 gtcccggctg tcgggaggtg ggaaatttac gggcggaatg cgtatcgttt gcgtgcgaat
     7681 cacctgcaaa aggagcaacc cctcagatgt cgtctgcgaa cccgaactcg ccttccgttc
     7741 ctgactcgca gccgcgtcag gtcatgaacg tggcggtgct ggctgagtcc gagctcggct
     7801 cggaggcaca gcgggagcgt cgcaagcgca tcctggacgc caccatggcc atcgcgtcca
     7861 agggcggcta cgaggcggtt cagatgcgcg cggtcgcgga ccgggccgat gtggcggtcg
     7921 gaacgctgta tcggtatttc ccgtcgaagg tgcacctgct ggtgtcggcc ctgggccgcg
     7981 agttcggccg catcgacgca aaaaccgacc ggtccgcggt taccggggcc acgccgtttc
     8041 agcggctgaa cttcatggtc ggcaagctga accgcgcgat gcaacgcaat ccgctgctca
     8101 ccgaggcgat gacgcgtgcc tacgttttcg ccgacgcgtc ggcggccagt gaggtcgacc
     8161 aggtcgagaa gctgatcgac agcatgttcg cgcgtgcgat ggccgacggc gaaccgaccg
     8221 aggaccagta ccacatcgcc cgggtgatct cggatgtgtg gttgtcgaac ctgctcgcct
     8281 ggctcacccg gcgcgcctcc gcgaccgacg tcagcaaacg gctggacctg gccgtgcggt
     8341 tgttgatcgg cgactccgaa aagacctgag cacccactgt ccgtgcagac ctaggctggc
     8401 ggcccggcgg tgcggccccg aatcagctcg gtgtccagca gttcgacgac cggcaggcca
     8461 ggcaggcccg tccgtggcgg cttgagcagc agatcacccg cccgctgacc cttctgcagg
     8521 ctcggctgcg ccaccgtggt cagcccgcga ctgatggcct cgggcacgcc gtcgaaacct
     8581 gtgacgctca tctgaccggg cacgtaaatg ccatgtgcgc gaaggtaatc catggcggat
     8641 aaggccagca cgtcggcggt gcacatcagc gccgtgattc gcgggttagc ctccagcgcc
     8701 accttggcgg cggcgccacc cgacgttggc aggttttcgt agctttctac caccgtgagc
     8761 gattccgggt ggacgccagc ggccttcatc gcctcccaca cgccgatgat gcgttcgcgt
     8821 tgcacatcga aggccggcga ctggagccgg tcgccgtcca ccagaccctg ccgacgatcc
     8881 ctacccagcc gcatggtcag caaaccgatc tcccgatgcc ccaacccgag tacgtagtcg
     8941 gcgatctcgc gcatcgccgc cctgtcgtca atacccaccc gggacacccc ggaaaggcct
     9001 ttgggctggt ccactattac gaccggcaaa cgccgctgca gcaccacctg caggtaggga
     9061 tcgtcgtcgc aggccgaata cacgacgaag ccatccacac cggcagacag cacggctgct
     9121 gtcccgtcct ccgtgctacg gcctggtccg acggccacca acagcagtcc ctgccccagc
     9181 tcttcgcacg attgcgccac gcccgcaacg aaatcccgcg ccgccggatc gctgaagaaa
     9241 taggtcagtg gttcggccat cagcaaaccg accgcacccg ctttgcgggt ccgcaacgat
     9301 cgcgccagcg gatcgggtcc cgcgtaaccc agccgcttcg ccgtggcgag cacccgttcg
     9361 cgcagctcgg gcgagagctg atccggtcgg ttgaaggcat tcgagatcgt ggtgcgcgaa
     9421 acattcagtt cgtctgccaa cgacgccaga gtcgcatgcc ggcgcgggat gggactcacg
     9481 tcgggtgagg ctacagccga tcgctgtgta cgcacagatc ggcaccgccg atccgcccga
     9541 ggccgcccgt cgcgaccgtt ccattcgcga tctgcctgtt ttgctggctg gtgagccgaa
     9601 gtcgggagcc cgccacttaa gcttgtcgca ccaggtggcc agcggtcagg ggaggcaaca
     9661 gtgcgccagc aactagccgc cctcgtgctg ggcggggtgt cggcgttggc catcggctgc
     9721 ggcaccacgg tcaacggcag ggccgtcgcc gccgacacct ccgggccccg gagccagaat
     9781 cccgttgcgg tgtcggcgct tgacgggctg ctgctcgacg cgggccagat caacgccgcg
     9841 ttgggcgcga catcgatgaa cgtgtggttt gccgccaatg cgatgtggga ttggggctcc
     9901 ggcgtcaacg acaagagctg cctggcgatc gacgggcccg cgcaagacca ggtctatgtc
     9961 aactcggggt ggacggcgat gcgcggccag cggctcgacg acagcgccga cgactccaag
    10021 aagcgcgacc actacgcgat ccaggccgtg atcgcgtatc cgtccgcgcg cgaagccaac
    10081 gcgttctacg actcctcgga gcaaagctgg tcggcgtgct ccaaccgccg gttttccgat
    10141 gccactccgg gccaacccga cacggtgtgg acggtgagcg gcttaaccac ggacaacggc
    10201 acgctcagta cctcccaggt ccaggaaggc ggggacgggt gggcctgcca gcgtgcgctg
    10261 acggtgcgca acaacgtcgc catcgatatc gtggcgtgcg cgttcaacca atccggtggg
    10321 cccgcggtcg acgttgctaa ccagatcgcg gccaaggtgg caaagcagta ggcgatgcgg
    10381 cggcttttgt gtgccgttcg gacggcaacc tgcgcggtgg cgatggccgc cacttggtgc
    10441 accggctgcg accggacggt cgagggcgcg gcgtttgccg cgcggacccc ggccagtgcc
    10501 gcggtgacga cgacggtcaa cccgtcgccg agcccgctgc cggtggcggg cctggagagc
    10561 ctgctgctga ccgccgacca gctttccgcc gtggtcggcg gcgcgaacat ggcgttgatc
    10621 ggcacggcca cgagcacatc ggacgcctcc aaactcatcg acgatcgcac atgcctggga
    10681 ctgggttcgg tcggagatgt ggtgagttat tccaacagtg ggtacgtcgc gatgcgcggc
    10741 aatcagttca gcacacccag cgccgtgcag gccgacgtca cgcagctggt gacatcgttc
    10801 accaccgccg ccgacgcgca ggcgctactg cagcgcacgc ggcaggattg gcaaggctgt
    10861 gcgggccggc gatacggatt tcactcctcg aacggcaacc acagctactt cgacaccggt
    10921 gacctgcgcg gtagcagttc gcgcatcgag gtgtcgatga gccaggaaga ggacgcccga
    10981 tggggatgct cgcacgcact ggccgtcggc aacaacatcg tcgcggaggc gcgggtctgc
    11041 cagctcggca aggacaccgc cgtggcggtc aacggcctcc tcgaccagat cgtggccaag
    11101 atcccgcaat aagctaggca ggtggccggc atcgacctca actccgactt gggcgagagc
    11161 tttggcgtct ggcgcctcgg tgatgacgac gccatgctcg ggatagtcac cagcgccaac
    11221 gtcgcgtgcg gtttccacgc gggagacccc gccggcctgt tgcgggtgtg ccggtcagcc
    11281 gccgaacgtg gcgtgcgtat cggggcgcag gtgagttatc gggacctggc cggcttcggc
    11341 aggcgcttca tcgacgtggc caccgaagac ctcgtcgccg acgtcgtgta ccagatcggc
    11401 gccctgcagg cgatcgcaca cacgtccggt tccgcggtgt cctacgtcaa accccatggc
    11461 gcgctgtata acacgatcgt taccaatcgt gagcagggcg ccgcggtcgc ggaggcggtg
    11521 cgcatggtgg atcccacact gccggtcctg ggcatggcgg gctcggcctt cttcgacgag
    11581 gcgacccgcc gtggtctgcg aaccgtggcc gaggcgttcg ccgaccgcgc ctaccggccg
    11641 gacggtcggc tggtctcccg tcgcgaaccc ggtgcggtgc tgcacgatcc ggcggcgatc
    11701 gccgaccggg tggtgagcat ggtgacctcg gggcacgtcg cggcgaccga cggcagccag
    11761 gttgccgtca gcgtggaatc ggtttgcgtt catggtga
//
LOCUS       DASSGQ010000340        24493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_236785,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000340 DASSGQ010000000
VERSION     DASSGQ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 24493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24493
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236785"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /gene="galT"
                     /locus_tag="VFI55_14925"
     CDS             <1..733
                     /gene="galT"
                     /locus_tag="VFI55_14925"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HET7075354.1"
                     /translation="AHGRSEVICFSSDHTGSFAQLAPAHARLVVEAWRHRTTELMATP
                     GIEQVFCFENRGEEIGVTLTHPHGQIYGYPYLTPRTAAMLAQARDHRMRDGCNLFADL
                     LGREVADGSRVVARTDLFTAFVPFAARWPVEVHIYPNRFVHNLVELDEGELDGFAQIY
                     LDVLGRFDRMYSAPLPYISALHQFSAGDEQADGYFHVELMSIRRAAGKLKYLAASESA
                     MDAFISDVTPESVAQRLRDLGGQLA"
     gene            730..1824
                     /locus_tag="VFI55_14930"
     CDS             730..1824
                     /locus_tag="VFI55_14930"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET7075355.1"
                     /translation="MTVRYAAPGRINLIGEHTDYNLGFALPIALPQRTVVTFTPDRTD
                     SITVHSQRTQGAFRFPVGTFPGQVTGWAAYVAGVVWALRQAGHPVPGGVMSISSDVAM
                     GSGLASSAALECAALGAIVSAAGGAPIDRIEEARLAQRAENDYVGAPTGLLDQLASLF
                     GEPSKATLIDFRELTVTPVPFDPEPSGVVLLLIDSRARHQHVGGDYAARRASCERAAA
                     DLQAASLRDVWDRGRAALDTVGNPIDARRARHVLTENGRVLDFVATLGDSDFVEAGRI
                     LTASHVSMRDDFNITTEHIDLIADTAVRAGAFGARMTGGGFGGCVIALVPERRADAVA
                     ETLRQTVIAAGHPRPVITRTWADAGAGTPT"
     gene            complement(1853..2560)
                     /locus_tag="VFI55_14935"
     CDS             complement(1853..2560)
                     /locus_tag="VFI55_14935"
                     /inference="COORDINATES: protein motif:HMM:NF004858.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HET7075356.1"
                     /translation="MNTPVEVEISDGIARLTLNSGKVNELSIQTFAALNAALDEAAAR
                     GAVVILTGQPGIFSGGYDLKVMRSGPEAAAELVTAGSSFSRRLLSHPHPVIAACSGHA
                     VANGAFILLSTDYRLGVDGDFKIGLNETAIGMTMHQVGIELARARLTPAAFNRAVLNA
                     EMFDPPAAVTAGFLDRVVDADTLADASTQVAQEMLKLNLVAHRHTKLKARKPLLATLD
                     TAIKNDRAQILMGSEFR"
     gene            complement(2609..3568)
                     /locus_tag="VFI55_14940"
     CDS             complement(2609..3568)
                     /locus_tag="VFI55_14940"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017835221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HET7075357.1"
                     /translation="MTRLFGIERPVMQGGMHWVGFAEMAAAVSNAGGLGIITGLTQRS
                     GENLAKEIARCRDMTDKPFGVNLTFLPSVTPPDYPGFIEAIINGGVTIVETAGNNPKQ
                     HLRTLKQAGITVIHKCTSVRHALKAQEIGCDAVSVDGFECAGHPGEGDIPGMILLPLA
                     GNVLEIPYIASGGMGDARSLVAALAMGAEGINMGTRFIATAEAPVHDNVKQALLAATE
                     YDTRLIMRSLRNTERVLTNEGVERLLEKERTLGDKLVFEDIIAEVAGVYPKVMRDGEM
                     DAGAWSCGLVASRIDDIPTVQELLDRIMAEAEDIIAGRLQGLL"
     gene            complement(3600..3755)
                     /locus_tag="VFI55_14945"
     CDS             complement(3600..3755)
                     /locus_tag="VFI55_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075358.1"
                     /translation="MFLDSTFHRAPNETDTLRHTNAELTSGATTPDNEFCPERATCAG
                     CMVVRWN"
     gene            4057..5883
                     /gene="fadD11"
                     /locus_tag="VFI55_14950"
     CDS             4057..5883
                     /gene="fadD11"
                     /locus_tag="VFI55_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase FadD11"
                     /protein_id="HET7075359.1"
                     /translation="MTPTERASTMCEAFQRTAAIDPDAIALRTPGGTQSLTWREYAAQ
                     VRRVAAGLAGLGVRRGDTVSLMMANRIEFFPLDVGAQHVGATSFSVYNTLPVEQMTYL
                     FENAGTKVAICDEQYVDRVRASGASIEHIVCIDGSPPGTLSVDDLYAAASGDYDFEST
                     WRAVQPDDVITLIYTSGTTGNPKGVEMTHTNLLFEGYALDEVLGIRFGDRITSFLPSA
                     HIADRMTALYAQEMFGTQVTVVTDARAIVTALPDVRPTVWGAVPRVWEKLKAAIEFTV
                     SHEKDDAKRQALQWGMSVAAKRGDALLAGVAMSDEAAAEWAKADELVLSKIRERIGFG
                     ELRWAISGAAPIPKETMAFFTGIGIPITEIWGMSELSCVATAIHPRDARPGTVGKPLP
                     GLEGKIADDGEFLVRGPLVMKGYRKEPAKTAEAIDADGWLHTGDIFEADSDGLLRVVD
                     RKKELIINAAGKNMSPVNIENAILAATPMVGVMIAIGEGRPYNTALLVFDADSVGPYA
                     AQHGLSDASPAALAADSEVIAQIAAGVAAGNQKLSRVEQIKRFRILPTLWEPGGDEIT
                     LTMKLKRKPIMTKHASEIEELYSSKPGPEIHEPAAAPPAQPA"
     gene            complement(5915..6310)
                     /locus_tag="VFI55_14955"
     CDS             complement(5915..6310)
                     /locus_tag="VFI55_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HET7075360.1"
                     /translation="MVIYNCTTTESTLSAQQKCELANEIIRIHSAINHVPVRYINVVF
                     NEQPGDNVYTGGVPASPLLVSSLFRSGHPESETTRLVGEIADAVTRVTGIPGERIISV
                     AQTTPTQFAFVHGRFFPDPSEEDASAAND"
     gene            complement(6540..7025)
                     /locus_tag="VFI55_14960"
     CDS             complement(6540..7025)
                     /locus_tag="VFI55_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007767988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075361.1"
                     /translation="MAEQSYAVDVEHPPSALIRVVNPLLRILLRTPLAGPAGKQFMVV
                     SFTGRKTGRQYSIPVSAHLIDHHLYALTGAPWKQNFRDGAPAQVVYDGKTAAMRGELI
                     RDRAVVADLYHRCAEAYGVQRAQRMMGMKFREPKIPTRDEFDEAVGRLHLTAIGFTAA
                     Q"
     gene            complement(7257..7940)
                     /locus_tag="VFI55_14965"
     CDS             complement(7257..7940)
                     /locus_tag="VFI55_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015292284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqN/LpqT family lipoprotein"
                     /protein_id="HET7075362.1"
                     /translation="MKHLTAATITVALSLALVGCGSENKTSPSTSTSTSVSTTATSGT
                     SGTSATSATPGGQANKTIADYIVETHITETPVHHGDPGPNVDLPVPAGWQINQNAGTS
                     YGGIVLTQPANPADPPTISALLSKLTGDVDAAKIIQYAPGEVQNLPGYQGSGNGEAST
                     LGGFQAWQLNGTYQKDGNTRAVAQKTVVIPSQGAVFVLQLNADSQQSDQGPLMDASNI
                     IDAQTTITP"
     gene            8108..10741
                     /locus_tag="VFI55_14970"
     CDS             8108..10741
                     /locus_tag="VFI55_14970"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HET7075363.1"
                     /translation="MRSRRALIALVAMTAVFMAFIAAAPPHAYDGEPQLVANPVDHVD
                     TLIGTGTGGETVGEIDNFPGATVPFGMVQYSPDTVGNYAGYNYDNPRSTGFSMTHASV
                     GCAAFGDISMLPTTSPIGTQPWNAWERIAHDDTEQGAPGYYSVRFPDTGVTAELTATT
                     RTGVGRFRYPRNGRPALFHVRSGGSLAGNSRATMQIGEDNTTITGWATSGGFCGKNNT
                     YTVYFAMKFSRPFTSYGSWDGYFVYPGARSAASPYGGGYVEFPAGSTVEVRTAISYVG
                     VDGARANLAAEGGAGFDAVRAAAMSQWNAALSSVTVAGRNLGNLATFYTSLYRSLLNP
                     NTFNDADGRYIGFDGVIHTVASGRTQYANFSDWDTYRSLAALQALLFPKQASDMAQSL
                     VNDAEQSGSFPRWAFANSATGEMSGDNVVPLIVNLYVYGAKDFDVHAALRYMVDGARK
                     GGVGLGGYVERPGIATYQALGYAPHTLEFGNNGWIADASITLEWSVDDFAISRFAESL
                     GDAATAAEFQNRAQYWQNLFNPSTHYISPRSTMGLFSTGPGFVDTPSTFGQDGFEEGN
                     AEQYLWWVPHNVVGLATALGGRAAAAGRLDRFTKNLNVGPNEPYLWAGNEPGFGVPWL
                     YNYLGQPWKTQLTVDRVRGLFGPVPDGEPGNDDLGAMSSWYVWAALGLYPSTPGTPNL
                     TVTAPLFDRVVIALPAGKSIRISAPGASGPHHLKYIGGLSVDGQATDHTALPESIIGA
                     GGDVTFSLAAYPDEVWGTAESSAPPSYGARSSAVTVNVSRPIVTIAPGSSGTVTLDAQ
                     RMIDGPGGYTITGTSDSGIRATPATGHFGPDGSAAVDVSISVAPSVPEEYYLVYLDTT
                     VGESIRRSVVLVVALAKAAEP"
     gene            complement(10749..13148)
                     /locus_tag="VFI55_14975"
     CDS             complement(10749..13148)
                     /locus_tag="VFI55_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET7075364.1"
                     /translation="MRVDGRDISVSGSLLQPLTRRTNDIIRLVLATIFLAVVITSSLV
                     TRPRWIALEKSISKVVGVLTPTQSDVVYVAYGFAILALPFVILIGLIVARQWKLLGAY
                     AAAAVLAVLPLSISSNRIAAFRWHFDVSDRLSTVPAQFLDDPRWIAMLAAVLTVSGPW
                     LPARWRHWWWALLLAFVPIHLIVSAIVPARSLVGLAVGWFVGALVVLVVGTPALEVPL
                     DGAVRAMGKRGFLVTALRVVRPAGSGPLVLAASAWDHDGIASGAQAEATIELYGPHQR
                     SGGALVQLWRKLRLRGSETAPLVASMRRAVEHRALMAIAIGDVGVANTSTIAVASLDR
                     GWMLYAHKPIRGTALDECAKTTPVERAWESLRILNEHRISHGDLCRDQITVDDGTVLF
                     GGFGSAEYGATDIQLQSDIAQLLVTTSALYNAESAVGAAIGAFGKDTVLTASRRLTKS
                     AVPKQIRQSIRDAGSVISGTRAEVKRQTGADQIKTETITRFTRSQVIQLVLLGALVYV
                     AYPFISTVPTFFSEVRRANWWWALLGLVISALTYVGAAAALWASADESVNFWKLSIAQ
                     LANTFAATTTPAGVGGLALSTRYLQKGGGLSAVRATTAVALQQSVQVIMHLVLLILFS
                     TVAGASMDLSHYVPDATVLYLIAGVALGIVGTFLFVPTLRRWLATDLRPRMKEVAGDL
                     VEVAREPKRLGLIVLGCAGTTLGSALALWTSVEAFGGGATFVTCTVVTMVGGTLASAA
                     PTPGGVGAVEAALIGGLAAFGVPAAVGVPSVLLYRVLTCWIPVFVGWPVMRWLTANEM
                     I"
     gene            13319..14275
                     /locus_tag="VFI55_14980"
     CDS             13319..14275
                     /locus_tag="VFI55_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007767980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7075365.1"
                     /translation="MARTDNDSWEITESVGATALGVAAARAAETESENPLISDPFARV
                     FLDAAGDGMWSWFAAPDLPAEIVEAEPDLKPRMQGMVDYMAARTSFFDQFFLDATRTT
                     RPGVSQVVILAAGLDSRAWRLPWPDGTTVYELDQPRVLEFKSSTLAENGAQPTCNLVH
                     VPVDLRHDWPAALQEAGFDGSAPSAWSAEGLLPFLPAAAQELLFERVQALSAPGSRIA
                     VEAPGPDFLDEDAEAKQRETMQRVRDLMAKLEPQRDIPDVQDLWYFEEREDVGDWLRR
                     HGWAVTVTEAVDLMARYDRRPPQGIDDAAPRTLFVAAERAGE"
     gene            14283..15236
                     /locus_tag="VFI55_14985"
     CDS             14283..15236
                     /locus_tag="VFI55_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7075366.1"
                     /translation="MSNARTDDDSWEITESVGATALGVASARAAETRSENPLISDPFA
                     QVFLDAAGDGVWNWYSAGQLPAELIEAEPRLPLQMQAMVGYMASRTAFFDRFFLDATR
                     AGIRQAVILAAGLDARSWRLDWRDGTTVYELDQPRVLEFKSSTLAAHGAQPACHRVAV
                     PVDLRHDWPAALQQAGFDPSAPSVWSAEGLMPYLPAAAQELLFDRIQGLAAAGSRVAV
                     EALGPKFLDPEFRTKRRERMDRIRELMAEVAPDREIPRTDELWYFEEREDVGDWFRRH
                     GWDVTVTPSSELMAGYGRAPRPEVEAEVPGNLFVAAQRAGS"
     gene            15410..16132
                     /locus_tag="VFI55_14990"
     CDS             15410..16132
                     /locus_tag="VFI55_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HET7075367.1"
                     /translation="MALQPVIRRSVPEEVFEQIAADVLSGEMQPGEALPSERRLADVL
                     GVSRPAVREGLKRLSAAGLVEVRQGGVTTVRDFRHHAGLDLLPRLLFRGGELDVSVVR
                     SILEARLRNGPKVAELAAERHGPELAELLGKSVRALQTEDDVLEWQLQTLKFWDHVVD
                     GAGSIVYRLMYNAFRAAYKPALDVVGTAMPAEVKCPDAYRELADAICSGDPAGAKRSA
                     EEVLELANTTLLAALEGLGERR"
     gene            16209..17003
                     /locus_tag="VFI55_14995"
     CDS             16209..17003
                     /locus_tag="VFI55_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7075368.1"
                     /translation="MTTNTVAGYLRGQVQPGIDAVGGFYRTCVLTGKALFRRPFQWRE
                     AIEQGWFITSVSLLPTLAVAIPLTVLIIFTLNILLAEFGAADISGAGAALGAVTQLGP
                     LTTVLVIAGAGATAICADLGARTIREEIDAMEVLGIDPIHRLVVPRVVAATIVATLLN
                     GAVITIGLVGGFIFGVFIQHISAGAYVGTLTLVTGLPEVVISVIKSATFGLIAGLVGC
                     YRGLTTKGGPKGVGTAVNETLVLCVIALFAVNVVLTTIGVRFGTGR"
     gene            17005..17892
                     /locus_tag="VFI55_15000"
     CDS             17005..17892
                     /locus_tag="VFI55_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7075369.1"
                     /translation="MAPTAESSAALVLRARYPRTAAGLNRFGGGAARRLEQIGIFARF
                     TRISVTQIWWALHRYRRETLRLTAEIGMGTGAMAVIGGTVAIIGFVTLSGSSLIAIQG
                     FASLGNIGVEAFTGFFAALANTRIAAPIVAGVALAATVGAGATAQLGAMRISEEIDAL
                     EVMGIKSISFLVSTRILGGLVVIVPLYALALDMAYTSGQIVTTVFYGQSNGTYQHYFR
                     TFLRPEDVGWSVVQVIIIAVVVMITHCYYGYTASGGPVGVGQAVGRSMRLSLVSVVVV
                     VLLAELALYGVDPNFNLTV"
     gene            17907..19121
                     /locus_tag="VFI55_15005"
     CDS             17907..19121
                     /locus_tag="VFI55_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HET7075370.1"
                     /translation="MTTPTTLQNRRVLLYAEGVVLLLIGALVLVLVYLQFRGDFTPKA
                     ELTLVASRAGLVMDPGSKVTYNGVQIGRVAGISEIESDGKPAAKLVLDVDPKYLNLIP
                     VNVVASIEAATLFGNKYVSLTSPENPQQQRISPHDVIDVRSVTTEFNTLFETITSIAE
                     KVDPIELNATLSAVAQALDGLGANFGDSIVDGNHILAQLNPRMPQLNHDVRGLADLAD
                     IYARSSPDLWGFLQNAVTTARTLTRQQGDLDAALLAAVGAGKTGEDIFSRGGPYLARG
                     AADLVPTAALLDTYSPELFCMVRNFHDAAPQVANAVGGNGYSLAAAGTVLGAPNPYVY
                     PDNLPRVNAHGGPGGKPGCWQTITRELWPAPYLVMDTGASVAPYNHFALGEPMFGEYV
                     WGRQYGENTINP"
     gene            19118..20149
                     /locus_tag="VFI55_15010"
     CDS             19118..20149
                     /locus_tag="VFI55_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET7075371.1"
                     /translation="MKTTGKAIKLGILWLVLLMFTVTIVVVFGQVRFNHTTGYSALFT
                     HVSGLRAGQFVRAAGVEVGNVSKVTLIDGDQRVLVDFAVDSSLPLDQATTASVRYLNL
                     IGDRYLELKHGDSGQRLTPGATIPLEHTKPALDLDALIGGFRPLFRALDPDKVNSIAQ
                     SIITVFQGQGATIDDILDQTASLTSTLADRDQAIGEIITNLNTVLATTVKHQQEFDQT
                     IDKLEVLITGLKNRADPLAAAAAHISDAAGTLADLLGEDRPLLHSTIGHLEDVQQPII
                     GQLASLDDVLRKLPEAYRIIGRSGGTYGDFFNFYLCDISLKVNGLQPGGPVRTIKLFG
                     QPTGRCTPQ"
     gene            20146..21693
                     /locus_tag="VFI55_15015"
     CDS             20146..21693
                     /locus_tag="VFI55_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HET7075372.1"
                     /translation="MRTLTEFNRGRMGLMGVVITLLAIGVGQSFTSVPMLFATPTYYA
                     EFVDSGGVNTGDKVEIAGVDVGLVRSLAIRGKRVLIGFSLAGKTIGRQSRAAIRTDTL
                     LGRKNMEIEPHGSEPLRPNGFLPSEQTTTPYQVYDAFVDATKAAAGWDVDTTKRSLQV
                     LSETFDETAPHLSAALDGVKAFSDSIGQRGDQLKQLLANANKVARVFGDRSEQVNGLL
                     VNANTLLAAFKERSQALSMLLSNVSGVSTQVSGLLKENPNVNHVLQQLNTVSDELVKR
                     KQDLADVLVLLSRYTAALTEAVGSGPYFKVILANLLPYQILQPWVDAAFKKRGIDPEQ
                     FWRSAGLPAFRFPDPNGTRFANGAPPPAPPVLEGTPDHPAPAVPPGSPCSYTPPADGL
                     PRPGNPLPCAASDQGPFGAVPGGFPAPLDVETSPPHADALPPAPGVPIAGRPGETPPA
                     APGTPTPLPADAPPGARTEPLGPVAGPNPPLPAPPVPAPGWAPPGPGPQLPAPFITPG
                     GTGGSSQ"
     gene            21690..23222
                     /locus_tag="VFI55_15020"
     CDS             21690..23222
                     /locus_tag="VFI55_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Mce family protein"
                     /protein_id="HET7075373.1"
                     /translation="MSTIFDIRNLRLPNVSRRVIVAGALVAVLAIVGVLAGRELYRKL
                     TTTSVVAYFSEAIALYPGDKVQIMGVRVGSIDKIEPAGDKMRITLHYSDKYKVPANAT
                     ASILNPSLVASRTIQLSPPYSDGPVLTDGAVIPIERTQVPVEWDHLRDSINGILRQLG
                     PTPEQPKGPFGDVIESAADNLAGKGEKVNETLSSLSEALTALNEGRGDFVAITRSLAQ
                     FISALYRNDQQFVALNGNLAQFTGWFTESDHDVADTLKRLDDVLGNVRKFVSDNRSTL
                     ARDVNNLADVTNTILQPEPRDGLETALHVLPTFAGNFNNIYSPLHSSLVGLFVFPNFA
                     NPIQFICSAIQAGSRRGYQDSAELCAQYLAPVLDAIKFNYLPFSANLFSSATTLPKDV
                     GYSEERLRPPPGYKDTTVPGIFSRDTPFSHGNHEPGWVVAPGMQGTPVQPFTANMLTP
                     ESLAELMGGENIAPPPPGNNLPGPPNAYDEGNPLPPPWYPQPAPPPPAAAGTAGQTGP
                     GQ"
     gene            23255..24352
                     /locus_tag="VFI55_15025"
     CDS             23255..24352
                     /locus_tag="VFI55_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Mce family protein"
                     /protein_id="HET7075374.1"
                     /translation="MVPVVLTSCTWRGIANVPLPVGHGAASGHMTIYVQMPDTLALNT
                     NSRVRVADVWVGTVRDIRLKNWIATLTLDVDPAVRLPANATAKVGQTSLLGTQHVELA
                     APPNASLQPLKSGDTIPLMNSSAYPTVERTLASVAIILNGGGIANLDVIQTEIIKILD
                     GHADQVREFLGRLATFTAALNRQRDDLTRAIDSTNQLVTIVANRRDTLDRVLTDIPPL
                     IKHFADTRDLFANATESLGRFSDVANRTLADTRPSLHQSLQSLQRPLNQLGRAAPYLI
                     GALRLGLTAPFNIDDVSKVVRGDYVNVSAAVDLTLSTIDNAILSGTGYSGALRALEQA
                     WGRDPATMIPDVRYTPNPNDAPGGPLVERGD"
     gene            24356..>24493
                     /locus_tag="VFI55_15030"
     CDS             24356..>24493
                     /locus_tag="VFI55_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075375.1"
                     /translation="MLTRFIKTQLVLLVVVAVAAVVLLGWYFLRLPSLVGVGRYTLYA
                     QL"
BASE COUNT         4181 a         7996 c         8106 g         4210 t
ORIGIN      
        1 ggcgcacggc cgtagcgagg tgatctgctt ttccagcgac cacaccggtt cgttcgcaca
       61 gttggcaccg gcgcatgccc ggctggtcgt cgaggcctgg cggcaccgca ccaccgagct
      121 gatggccaca ccgggcatcg agcaggtgtt ctgcttcgaa aaccgcggcg aggaaatcgg
      181 ggtgacgctg acccatccgc acggtcagat ctacggctac ccctacctga cgccgcgaac
      241 cgccgccatg ctggcccagg cgcgcgacca tcggatgcgc gacggctgca acctctttgc
      301 cgacctgctg gggcgcgaag tcgccgacgg cagtcgcgtt gtcgcgcgca ccgacttgtt
      361 taccgccttc gtgccgttcg cggcacgctg gcccgtcgaa gtgcacatct acccgaacag
      421 gttcgtgcac aacctcgttg agctcgacga gggcgaactg gatggattcg cgcagatcta
      481 cctcgacgtg ctcgggcggt tcgaccggat gtactcggct ccgctgccgt atatttcggc
      541 gctgcaccag ttcagcgccg gcgacgaaca ggcggacggc tacttccacg tcgagttgat
      601 gtcgatccgg cgcgccgccg gcaagctcaa gtacctggcg gcctccgagt cggcgatgga
      661 cgcgttcatc agcgacgtca cgcccgaaag tgtggctcag cggctcagag atttgggagg
      721 gcagctcgca tgacggtgcg ctacgccgcg ccggggcgga taaacctgat cggtgagcac
      781 accgactaca acctgggctt cgcgctgccg atcgcgctac cccagcgcac tgtggtgacg
      841 ttcacgcccg accgcaccga ctcgatcacc gtgcacagcc agcgcacgca gggcgccttt
      901 cgctttccgg tcggcacctt ccccggccag gtgaccgggt gggccgctta cgtggccggg
      961 gtggtgtggg cgctgcgtca ggccggccac ccggtgcccg gcggtgtgat gtcgatcagc
     1021 agcgacgtgg cgatggggtc gggcctggct tcgtcggcgg cattggaatg cgcggcactg
     1081 ggcgccatcg tgtcggccgc cggcggcgca cctatcgacc gcatcgagga ggcgcggctc
     1141 gcgcagcggg ccgaaaacga ctacgtcggc gccccgacgg gattactcga ccagttggca
     1201 tcgctgtttg gagagccgtc gaaggcgacg ctcatcgact ttcgggaact caccgtcacg
     1261 cccgtgccgt tcgaccccga accttctggc gtcgtgctgc tgctgattga ctcccgtgcc
     1321 cggcatcagc acgtcggcgg cgactacgcg gcccgtcgtg catcgtgtga gcgggcggcc
     1381 gccgatttac aggcggcatc gttgcgtgac gtctgggatc gcgggcgcgc ggcgttggac
     1441 acggtcggca acccgatcga tgcccgccgc gcccgccatg ttctcaccga gaacggaaga
     1501 gtactggatt tcgttgcgac attgggtgat tcggacttcg ttgaagccgg acgcatcctg
     1561 accgcctcac acgtctcgat gcgtgacgac ttcaatatca ccaccgaaca tatcgatctg
     1621 atcgccgaca ccgcggtgcg cgcaggagct ttcggcgctc gaatgaccgg cggcggattc
     1681 ggcggctgtg tgatcgcgtt ggtgccggaa aggcgggcgg acgccgtcgc cgagacgctg
     1741 cgacagacgg tcatcgctgc gggtcacccg cggccggtga tcacccgcac ctgggcggac
     1801 gcgggcgccg gaacccccac ctgagcgagc cggggtggcg ccgatcggcg ggtcagcgga
     1861 attcgctgcc catcaggatc tgcgcgcggt cgtttttgat ggccgtgtcg agcgtggcca
     1921 gcagcggctt gcgggccttg agtttggtgt ggcggtgggc gaccaggttg agtttgagca
     1981 tctcctgggc gacctgtgtg ctggcgtcgg cgagcgtgtc ggcatcgacc acccggtcta
     2041 ggaagccggc ggtgacggcg gcgggggggt cgaacatctc ggcgttgagg accgccctgt
     2101 tgaacgccgc cggggtgagc cgggcgcggg ccaactcgat gccgacttgg tgcatggtca
     2161 tcccgatggc cgtctcgttg agcccgatct tgaagtcgcc gtcaacgccg agccggtagt
     2221 cggtggacaa caggatgaaa gcaccgttgg cgaccgcgtg acccgaacac gcggcgatga
     2281 ccgggtgcgg gtgggacagc aaccgacgcg agaagctgga ccccgcggtc accagctcgg
     2341 cggcggcctc gggtcctgag cgcatcacct tcaggtcgta accgccggag aagataccgg
     2401 gttggccggt gagaatgacc accgcacccc gggccgcggc ctcatccagt gcggcgttga
     2461 gcgcggcgaa tgtctggatg gacagttcgt tgaccttgcc gctgttcagg gtcagccgcg
     2521 cgattccgtc ggagatttct acctcgactg gtgtgttcat gatgcctccg tgatgggtag
     2581 gtcgtggtcg gctgccttac cgcagccctt acagcagccc ttggagccgg ccggcgatga
     2641 tgtcctcggc ctcggccatg atccggtcga gcagctcctg cacggtcggg atgtcgtcga
     2701 tccggctggc caccaggcca cacgaccagg cgccggcgtc catttcgccg tcgcgcatca
     2761 ccttcgggta gacaccggcc acctcggcga tgatgtcctc gaacaccagc ttgtcgccga
     2821 gggtgcgttc cttctccaac agccgctcga cgccttcgtt ggtcagcacc cgctcggtgt
     2881 tgcgcagcga gcgcatgatc agcctggtgt cgtactcggt ggccgccagc agcgcctgct
     2941 tgacgttgtc gtggaccggc gcctccgcgg tggcgatgaa ccgcgtgccc atgttgatcc
     3001 cctcggcgcc catcgccagt gcggccacca gggagcgggc atcgcccatc ccgcccgagg
     3061 cgatgtaggg gatttccagc acgttgccgg ccagcggcag caggatcatg ccggggatgt
     3121 cgccctcgcc ggggtgcccg gcgcactcga aaccgtcgac gctgaccgcg tcacagccga
     3181 tctcttgggc cttgagcgcg tggcgcaccg aggtgcattt gtggatcacc gtgatacctg
     3241 cctgcttgag ggtgcgcagg tgctgcttgg ggttgttgcc ggcggtctcc acgatggtga
     3301 cgccgccgtt gatgatcgcc tcgatgaacc ccgggtagtc cggcggggtg accgagggca
     3361 ggaaggtgag gttgacccca aatggcttgt cggtcatgtc ccgacagcgg gcgatctcct
     3421 tggccaggtt ctcccccgag cgctgggtga gtccggtgat gatgcccagt ccgcccgcat
     3481 tcgagaccgc ggcggccatc tcggcgaagc cgacccagtg catgccgccc tgcatcaccg
     3541 gccgctcgat gccgaacagt ctggtcatcg ctgtttgcat ggtggatcta cctttcttgt
     3601 cagttccagc gcaccaccat gcagccagcg caggtcgcgc gttcgggaca aaactcgttg
     3661 tcgggtgttg ttgcaccact ggtcaattcg gcattggtgt gcctcagcgt gtcggtttcg
     3721 ttgggagcgc gatggaaggt gctgtcgagg aacatggcgg tccgaaagtg tggctggccg
     3781 agccgacatt ccgaaccccg ggactgtcca gagccggcat cgatgtcctc ataatcttgc
     3841 accggagttc tgccgaagac gctatttcgc ggaccaatac ggacccgttt tcgttaggtc
     3901 tgaacacaag acttcatcac gaagcaatgg catcgtcatg gccatgaaaa agtcccaaag
     3961 cgctggtcac ggacctacgc tcagcgatgt agtgggcgat gccgaactgc tcaaaagtcg
     4021 gtaccgcgat cgaaagaaac tcaaaggaga acggaaatga ccccgaccga gcgtgcgtcc
     4081 accatgtgcg aggccttcca gcgcacggcg gcgatcgacc cggacgccat cgctctgcgg
     4141 acacccggcg gcacccagag cttgacgtgg cgcgagtacg cggcgcaggt gcggcgggtg
     4201 gccgccggcc tggcgggttt gggggtcagg cggggtgaca cggtttcgct gatgatggcc
     4261 aaccgcatcg agttcttccc gctcgatgtc ggcgcacaac acgttggcgc cacttcgttt
     4321 tcggtctaca acacgctgcc cgtcgagcag atgacgtatc tgttcgagaa cgccggcacc
     4381 aaggtcgcga tctgcgacga acaatacgtc gaccgtgtcc gtgccagtgg cgcgtccatc
     4441 gagcacatcg tctgcatcga cggttcgcct ccaggcacgc tgtcggtgga tgacttgtac
     4501 gcggccgctt cgggagacta cgacttcgag tcgacgtggc gcgcggtgca acccgacgac
     4561 gtcatcacgc tgatctacac ctcggggacc accggaaacc ccaagggtgt ggagatgacc
     4621 cacaccaacc tgctgttcga gggttacgcc ctcgacgagg tcctcggaat ccggttcggc
     4681 gaccgcatca cgtcgtttct gccctcggca cacatcgccg accggatgac cgcgctctac
     4741 gcccaggaga tgttcggtac ccaggtcacg gtggtcaccg acgcgcgcgc gatcgtgacg
     4801 gcgttgcccg acgtgcggcc cacggtgtgg ggcgcggtgc cccgcgtatg ggaaaagctc
     4861 aaggccgcaa tcgaattcac cgtgagccac gagaaggacg acgcgaaacg gcaggctctg
     4921 cagtggggga tgtcggtggc cgccaaacgc ggcgacgccc tgctagccgg cgtggcgatg
     4981 tccgacgaag cggccgccga atgggccaag gccgacgagt tggtgctatc gaagattcgg
     5041 gagcggatcg gcttcggaga actccggtgg gcgatctcgg gagccgctcc gatccccaag
     5101 gagaccatgg ctttcttcac cggcatcggc atcccgatca ccgagatttg ggggatgtcc
     5161 gagttgagct gcgtggccac cgcgatccat ccgcgggacg cgcgcccggg caccgtgggc
     5221 aagccgcttc ccggcctgga gggcaagatc gccgacgacg gcgagtttct ggttcgcggc
     5281 ccgctggtga tgaagggcta ccgcaaggag cccgccaaga ccgcggaggc tatcgacgcc
     5341 gacggctggc tgcacaccgg cgacatcttt gaagccgatt ccgacggtct tctgcgggtg
     5401 gtcgaccgga aaaaggagtt gatcatcaac gcggccggca aaaacatgtc accggtcaac
     5461 attgagaacg ccatcctcgc cgcaaccccg atggtcgggg tgatgatcgc aatcggtgag
     5521 gggcgcccgt acaacaccgc gctgctggtc ttcgacgccg actcggtcgg cccgtatgcg
     5581 gcccagcatg gcctctccga cgcctcgccc gcggccctgg cagccgactc ggaggtgatc
     5641 gcgcaaatcg ccgccggcgt cgccgcgggc aaccagaaac tctcccgggt ggaacagatc
     5701 aaacgttttc gcatcttgcc caccctgtgg gagccaggcg gcgacgagat aacgctcacg
     5761 atgaaactca agcgcaagcc gatcatgacg aagcacgcaa gcgagatcga ggaactctat
     5821 tccagcaaac cgggaccgga gatccacgag cccgctgccg cgccaccggc acaacccgcg
     5881 tgacgggggc cggcgggttg ggatgcgccc cctgtcagtc gtttgcggct gaggcgtctt
     5941 cttcgctcgg gtcgggaaag aaccggccgt gcacgaaggc gaactgcgtt ggggtggttt
     6001 gcgcgacgga aattattcgc tcccccggga taccggttac ccgggtgacg gcgtcggcga
     6061 tttcgccgac gagacgtgtg gtttcggact ccgggtggcc cgaccgaaac aggctgctga
     6121 ccaaaagcgg gctcgcgggc acaccgccgg tgtaaacgtt gtcgccgggt tgctcgttga
     6181 acacaacgtt gatgtatctg acgggcacgt gattgatcgc ggaatggatc cgaatgatct
     6241 cgttggccag ctcgcacttt tgctgggcac tgagcgtcga ttctgtggtc gtgcagttat
     6301 agatgaccat gctcgatcct tccagtcggg ctcatatcgc aaagctcttc tgtcgtaacg
     6361 cacctggtga agtcaccgtc tgttggcacg gtgcttgggt caaccggttc agcggtgaaa
     6421 tccccacata gggtgagaaa accaaccagg ttggggtggg gcggcactga gcgcgtgccg
     6481 ctgccgaact gagctccgct ggagctcagg taatcgcgtg cgagggtctc gtcgaaaacc
     6541 tactgggccg cagtgaaccc gatggccgtc aggtggagcc ggccgacggc ttcatcgaac
     6601 tcgtcgcgcg tcggtatttt cggttcgcgg aatttcatcc ccatcatgcg ctgcgcccgc
     6661 tgcacgccat aggcctcggc gcagcggtgg tacaggtcgg cgacgacggc gcggtcgcga
     6721 atgagctcgc cgcgcatggc ggcggttttg ccgtcgtaga cgacctgggc gggtgctccg
     6781 tcgcgaaagt tctgcttcca cggagcgcct gtcagcgcgt aaaggtgatg gtcgatcaga
     6841 tgggcactca ccggaatcga atactgccgg cccgtctttc gcccggtgaa gctcaccacc
     6901 atgaactgct tgcctgcggg acctgccaag ggggtgcgca gcagtatccg cagcagcggg
     6961 ttgacgacac ggattagtgc tgacggcggg tgttcgacgt ctactgcata cgactgctct
     7021 gccataccca ccaccctaag ggcgatcggc cgccgcggcg tacgccgaga tggccggcat
     7081 ggtcgtgctg ggagctcgga ccgcagccct cacggcgatc tcggcggttc gacgcgcagc
     7141 gaggaattga tggcccgata cggccgtggc gacccggagg ggctggcgga cggcgcgccg
     7201 cggagcgtgt tcgactccgc ggcgcgatcc gtcttgtcgt tgcggggcag gggtattcag
     7261 ggggtgatgg tggtctgggc gtcgatgatg ttgctggcgt ccatcaacgg gccttgatcg
     7321 ctttgctgcg agtcggcgtt gagctgcagc acgaacaccg caccttggct gggaatcacc
     7381 acggtcttct gcgcgaccgc ccgcgtattg ccatccttct ggtacgtgcc gttcagctgc
     7441 caggcctgga agccgccgag cgtggaggcc tcgccgttac ccgagccctg gtagccgggg
     7501 aggttctgca cttcgccggg ggcgtactgg atgatcttgg cggcatcgac gtcaccggtg
     7561 agcttggaga gcagtgccga gatggtgggc ggatcggcag gatttgcggg ctgggtcaaa
     7621 acgatgccgc cataggacgt tccggcgttc tggttgatct gccaacccgc gggcaccggc
     7681 aggtcgacgt tggggccggg atcgccgtga tggacgggag tctcggtgat gtgggtctcg
     7741 acgatgtagt cggcgatggt cttgttggcc tgcccgcccg gcgtcgcgga cgtcgcggag
     7801 gtcccggacg tcccggacgt cgccgtcgtc gagacggacg tggatgtcga tgtcgacggc
     7861 gatgtcttgt tctcagagcc gcaacccacc agcgccaaac tcagcgccac ggtaatggtt
     7921 gctgctgtca agtgtttcat ctatcaaatc tcccaggaat tgggccggta cctgcgccag
     7981 ccacgcggac tgagactaac cgaagtcgat ccccggcgtt gctacgtcgt gtgcgggccg
     8041 aaccgccgcc gtcagatccc tgtcgaaaca cccacgcgaa gaggcggcta tgcagtaaga
     8101 gacagctatg cggtcgcgaa gggccctcat cgcgcttgtc gccatgacgg ccgtcttcat
     8161 ggctttcatc gccgcggctc cgccacacgc atacgacggc gagccgcagt tggtcgccaa
     8221 tcccgtcgat cacgtcgaca cgctgatcgg caccgggacg ggtggtgaga cggtagggga
     8281 gatcgacaac tttcccggcg ccaccgtgcc gttcggaatg gtgcaatact cgcccgacac
     8341 ggtcggcaac tacgcgggct acaactacga caatccgcga tccaccggat tcagcatgac
     8401 ccacgcctcg gtgggctgcg ccgcgttcgg tgacatctcg atgctgccca ccaccagccc
     8461 tatcggcacg cagccgtgga acgcctggga acggatcgcc cacgacgaca ccgagcaggg
     8521 tgcgcccggc tactactccg tgcggttccc cgacaccgga gtgaccgcgg aactcaccgc
     8581 caccacccgc accggcgtcg gccggttccg ctatccgcgc aatggccggc ccgccctgtt
     8641 ccacgtgcgc tccggcggat cgctggcggg caattcccgc gcgaccatgc agatcggcga
     8701 ggacaacacc acgatcaccg gatgggcgac cagcggcggg ttctgcggca agaacaacac
     8761 ctacacggtg tatttcgcca tgaagttcag ccggccgttc acctcttacg gctcgtggga
     8821 cggctatttc gtctaccccg gggcccgcag cgcggcgtcc ccgtacggcg ggggttacgt
     8881 ggagttcccg gccggctcga cggtcgaggt gcgcaccgcg atctcctacg tcggtgtcga
     8941 tggcgcgcgg gcaaacctgg cggcggaggg cggggcgggc ttcgacgccg tgcgcgccgc
     9001 cgcaatgtcc caatggaacg ccgcgttgtc gtctgtcacg gtggcgggcc ggaatctcgg
     9061 caatctggcg acgttctata cctccctgta ccggtctctg ttgaacccca acactttcaa
     9121 cgacgccgac ggccgctaca tcggattcga tggcgtcatc cacaccgtgg ccagcgggcg
     9181 tacccagtac gccaacttct ccgactggga tacctaccgg agcctggccg ccctgcaggc
     9241 gttgctgttc ccgaagcaag ccagtgacat ggcccagtcg ctggtcaacg acgccgagca
     9301 gagcgggtct tttccgcgct gggcgttcgc gaattcggcc accggcgaga tgagcggaga
     9361 caatgtggta ccgctcatcg tgaatctata cgtttacgga gccaaggact ttgacgtcca
     9421 cgcggcattg cgctacatgg tggacggggc gaggaaaggc ggtgtcgggc tgggcggcta
     9481 tgtggagcgg ccgggcatcg cgacctacca ggcactgggc tatgcaccgc acaccttgga
     9541 gttcggcaac aatggctgga tcgcggatgc ctccatcacg ctggagtggt cggtcgacga
     9601 cttcgcgatc tcgcggttcg ccgaatccct cggcgacgcg gcgaccgccg ccgagttcca
     9661 gaaccgggcg cagtattggc agaacctgtt caatcccagc acccattaca tctcgccccg
     9721 cagcacgatg ggattgtttt ccaccggccc cggattcgtg gatacccctt cgactttcgg
     9781 ccaggacgga ttcgaggagg gcaacgctga gcaatacctg tggtgggtgc cgcataacgt
     9841 cgtcggtctg gcgacggctc tcggcggccg cgccgcggcg gcgggccggc tggaccgctt
     9901 caccaaaaac ctcaacgtgg gccccaacga gccttacctc tgggccggca acgagccggg
     9961 attcggggtg ccgtggctgt acaactacct cggccaacca tggaagactc agctcacggt
    10021 cgaccgggtg cgcgggctgt tcgggccggt gcccgacggc gagccgggca acgacgatct
    10081 cggggcgatg tcgagctggt atgtctgggc cgccctcggc ctgtacccga gcacacctgg
    10141 aacgccgaac ctcaccgtga ccgccccgct gttcgatcgc gtcgtaatcg cccttccggc
    10201 aggcaaatcc attcggatct ccgcgccggg cgcatcggga ccgcaccacc tgaagtacat
    10261 cggcggcctc agcgtcgacg gccaggccac cgatcacacg gcacttccgg agtcgatcat
    10321 cggcgccggc ggcgacgtca cgttttcgct cgccgcctac cccgacgagg tctggggcac
    10381 ggccgaatcc tccgcgccgc cgtcgtacgg cgcgcgcagt tcggcggtga ccgtcaacgt
    10441 ctcccgtccc atcgtcacga tcgcaccggg cagctcgggc accgtgaccc tcgacgcgca
    10501 gcgcatgatc gacggcccag gcggttacac gatcaccggc acgtccgaca gcgggatccg
    10561 ggcgacaccc gccaccggac atttcggacc cgacggatcg gccgccgtcg acgtgtcgat
    10621 ctcggtggcg ccgtcggtgc ccgaggagta ctacctggtc tatctcgaca ccacggttgg
    10681 cgaaagtatc cggaggtcag tcgttttggt cgtcgccctg gccaaggccg ccgaaccctg
    10741 acatgtcgtc agatcatctc gttcgccgtc agccaccgca tcaccggcca gccgacgaag
    10801 acgggaatcc agcaggtcag cacccggtac agcagcacgg acggcacccc cacggccgcg
    10861 ggcacgccga aggcggccag accaccgatg agtgcggctt cgaccgcccc gacgccgccg
    10921 ggcgtcgggg cggcagacgc cagcgttccg cccaccatgg tcaccacggt gcaggtgacg
    10981 aacgtcgcgc cgccgccaaa ggcttcgacg ctggtccaca gggccaacgc cgagccgagg
    11041 gttgttccgg cacagcccag tacgatcagc cccagtcgct tcggttcgcg ggcaacctcg
    11101 acgaggtcac cggccacctc cttcattctc ggacgcaggt cggtcgccag ccagcgccgc
    11161 agcgtgggga cgaacagaaa tgtgccgacg atgcccagcg ccacacccgc gatcaggtag
    11221 agcaccgtgg catcaggtac gtaatgcgac aagtccatcg aggcgcccgc gacggtgctg
    11281 aacaagatca gcaacaccag gtgcatgatc acctgcacgg attgctgcag cgccaccgcg
    11341 gtggtggccc gcaccgcgga cagccctcct cccttctgca ggtaccgtgt gctcagcgcc
    11401 agtccgccga ccccggccgg ggtagtggtc gccgcaaaag tgttggccaa ctgtgcaatt
    11461 gatagcttcc agaagttcac cgattcgtcg gcgctcgccc acaaagcagc tgcggcaccc
    11521 acatacgtca acgcggatat caccaagccc agcagtgccc accaccagtt cgccctcctc
    11581 acttcggaga agaacgtcgg gaccgtgctg atgaacgggt atgcgacata caccagtgct
    11641 cccagcagga ccagctggat gacctggctg cgggtgaacc gggtgatcgt ttcggtcttg
    11701 atctgatccg caccggtttg ccgcttcacc tccgcgcggg tgccggagat gacggagccc
    11761 gcgtcgcgga tcgattgccg gatttgtttc ggtacagccg atttcgtgag ccgccgcgac
    11821 gcggtcaaga cggtgtcttt gccgaatgcg ccaatcgcgg cgcccaccgc ggactccgcg
    11881 ttatacagcg ccgacgtcgt caccaggagt tgcgcgatgt cagattggag ttgaatgtcg
    11941 gtggcgccgt attcggcgct gccgaacccg ccgaacagca cggtgccgtc gtcgaccgtg
    12001 atctggtcgc ggcacagatc gccgtgggag atccggtgtt cgttgaggat tcgcaacgat
    12061 tcccacgcgc gttcgaccgg cgtcgtcttc gcgcattcgt cgagcgcagt tccccggatt
    12121 ggcttgtgtg cgtacaacat ccatccccgg tccaggctgg ccaccgcgat cgtggaggtg
    12181 ttggcgacgc ccacatcgcc gatcgcaatg gccatcagcg cgcgatgctc gacggcacgg
    12241 cgcatcgagg cgaccagagg tgcggtctcg ctgccccgca gccgcagctt gcgccacagc
    12301 tgcacaagtg cgccaccgct acgttgatgc gggccgtaca gctcgatcgt cgcctcggcc
    12361 tgggcgcccg acgcgatgcc gtcgtgatcc caggcggagg ccgcaagaac gagtggcccc
    12421 gaccccgccg ggcggaccac ccgaagcgcg gtcaccagga atccgcgctt gcccatcgcg
    12481 cgtaccgcgc catccagcgg cacctccagc gcgggcgtgc cgaccaccag caccaccagc
    12541 gcgccgacga accagcccac ggcgagcccc acgagcgagc gcgccggcac gatggcgctg
    12601 acgatcaagt ggatcggcac gaacgccaac agcagcgccc accaccagtg ccgccagcgg
    12661 gcgggcagcc aggggcccga cacggtgagc accgccgcga gcatcgcaat ccaccgcggg
    12721 tcgtcgagaa actgcgccgg cacagtgctg agccggtcgg agacgtcgaa gtgccatcgg
    12781 aatgccgcga tgcggttgct gctgatcgac aagggcaaga ccgcgaggac ggcggcggcc
    12841 gcgtacgcgc cgagcaattt ccactgccgt gcgacgatca ggccgatcag gatcacgaac
    12901 ggcagcgcca ggatcgcgaa tccgtacgcc acgtacacca catcggattg ggtaggggtc
    12961 aagaccccaa cgactttgga gatggatttc tccaacgcga tccaccgggg gcgggtgacc
    13021 agcgaactgg tgatcaccac ggcgaggaag atcgtcgcca gcaccagccg gatgatgtcg
    13081 ttggtccgcc gggtgagcgg ttgcagcaag ctgcccgaaa cgctgatgtc gcgcccgtca
    13141 actcgcatgc cgagttcctt cgagatcctg tgcaccggcg ccgcgtattt agcggcccgt
    13201 cgacaactta accggatacc tcggggccag cgcgcatgta cctgaaggac agctccttca
    13261 catgcggcgc gcgtagggtc gactaggtag ttgtgctagc gaagtggagg acgtgagcgt
    13321 ggccagaacc gacaacgaca gctgggagat caccgagagc gtgggcgcga cggcgctggg
    13381 cgtcgcggcg gcccgtgcgg cggaaaccga aagtgagaac ccgctgatca gcgacccgtt
    13441 cgcgcgggtg ttcctggacg cggccgggga cggcatgtgg agctggttcg ccgcccccga
    13501 cctgcccgcc gagatcgtcg aggccgaacc cgacttgaag ccgcggatgc agggaatggt
    13561 cgattacatg gccgcgcgca cgtcgttttt cgatcagttc tttctcgatg caacccgaac
    13621 gacacgcccc ggcgtgtcgc aggtggtgat tctggcggcg ggtttggact cgcgggcgtg
    13681 gcgactgccc tggcccgatg gcaccacggt ctacgaactc gaccagcccc gggtgctgga
    13741 attcaagtcc tcgacgctgg ccgagaacgg cgcgcagcca acctgcaacc tggtgcacgt
    13801 tccggtcgac ctgcgtcatg actggccggc cgcgttgcag gaggccggtt tcgacggctc
    13861 ggcgccgagc gcctggtcgg ccgaggggct gctgccgttc ctgccggcgg ccgcccagga
    13921 gttgctgttc gagcgtgttc aggcgctgag cgcgccgggc agccgtatcg ccgtggaggc
    13981 gcccggccct gacttcctcg acgaagatgc cgaggcaaag cagcgcgaga cgatgcagcg
    14041 ggtgcgcgac ctgatggcga aactggagcc gcagcgcgac atccccgacg ttcaagacct
    14101 gtggtacttc gaggagcgcg aggacgtcgg cgactggttg cgccgccacg gctgggccgt
    14161 gacggtgacg gaggctgtcg acctgatggc ccgatacgac cgcaggcccc cgcaaggcat
    14221 cgacgacgcc gcgccgcgga cgttgttcgt ggccgccgag cgggcggggg agtgagcgcg
    14281 gaatgagcaa cgccagaacc gatgacgaca gctgggagat caccgagagc gtgggtgcga
    14341 cggcgctggg tgttgcgtcc gcgcgcgcgg cggaaacccg cagcgagaat ccattgatca
    14401 gcgatccgtt cgcgcaggtc ttcctcgacg ccgccggcga cggggtatgg aactggtatt
    14461 cggctgggca gctgcccgcc gagctcatcg aggccgaacc ccggttgccg ctgcagatgc
    14521 aagcaatggt cggctatatg gcttcgcgga cagcgttttt cgaccggttc ttcctcgacg
    14581 cgacccgtgc gggcattcgc caggcggtga tcctggcggc gggcctcgac gcgcggtcct
    14641 ggcggctgga ctggcgggac gggaccaccg tctatgagct cgaccagccc cgggtcctgg
    14701 agttcaaatc gtcgacgttg gccgcgcacg gggcacagcc cgcctgccac cgggtcgcgg
    14761 tgccggtgga tctgcggcac gactggccgg cagcgttgca gcaggcgggt tttgacccgt
    14821 ctgcgcccag cgtctggtcg gccgaggggc tgatgccgta cttgcctgcg gcggcacaag
    14881 agttgctgtt cgaccgcatt cagggcctcg ccgccgccgg cagcagggtt gcggtggagg
    14941 cgctggggcc gaagtttctg gacccggagt tccgcacgaa gcgtcgtgaa cggatggatc
    15001 ggattcgcga gttgatggcc gaggtggcgc cggaccgtga gatccccagg acggacgagt
    15061 tgtggtactt cgaggaacgc gaggacgtcg gtgactggtt ccgccgccac ggctgggacg
    15121 tgacggtgac gccgtcctcc gagctgatgg ccggctacgg ccgcgcgccg cgccccgagg
    15181 tcgaggcaga agttccgggg aatttgttcg tggcggccca gcgggcgggt tcgtagcggc
    15241 cccaacgggg cagggcagag tcgaacccgc caccgggagg ttcgacgccc ggatcggcct
    15301 gggggcatcg cgacgttgac ggtcgccgcg cgccgtcgcg gcgacacacg cattggtaaa
    15361 tggtctgacc acttgacgtc tgacctctga tcagtcacac tttgggctca tggcgctgca
    15421 gccggtgatt cgtcgatcgg tgcccgaaga ggttttcgag cagatcgctg ccgatgtgct
    15481 gagcggggag atgcaacccg gcgaggcgtt gcccagcgag cgccgattgg cggacgtcct
    15541 gggggtctct cggcccgctg tccgcgaggg gctcaagcgg ctttcggcgg ccggtctggt
    15601 cgaggtgcgc cagggcggcg tcaccaccgt gcgtgacttc cggcatcacg ccggcctgga
    15661 cctgctgccc cggttgctgt ttcgcggcgg cgagctcgac gtctccgttg tccgcagcat
    15721 cctcgaggcc cggctgcgta acggtccgaa ggtcgcggag ctggcggccg aacggcacgg
    15781 acctgagttg gccgaactgc tcgggaagtc ggtacgcgcg ctgcagaccg aggacgatgt
    15841 gctcgagtgg cagttgcaaa ctctgaagtt ttgggatcac gtggtcgacg gcgccggctc
    15901 gatcgtatat cggttgatgt acaacgcatt tcgcgccgca tataagccgg cgctagatgt
    15961 ggtgggcacc gcgatgcccg ctgaggtcaa atgccccgat gcctaccggg aactggccga
    16021 cgcgatctgc tcgggtgatc ccgccggtgc gaagagatcc gccgaagaag tgctcgaact
    16081 cgcgaacacc acgttgttgg ccgcgctcga gggcctgggg gagcggcgat gagcgtgtcg
    16141 actgcgccgc gggtgatcgg tgccgcgaag cggcgcgcgg aaggtggttg tgccgaggga
    16201 ctgtgccaat gaccactaac accgttgccg gctatctacg cggccaggtc cagcccggga
    16261 ttgacgcggt cggcgggttc taccggacct gcgtgttgac cggcaaagcg ctgtttcggc
    16321 ggcccttcca gtggcgtgaa gcgatcgagc agggctggtt catcacgagt gtctcgttac
    16381 tgccgaccct ggcggtcgca attccgctga ccgtgctcat catcttcacg ctcaacatcc
    16441 tgctcgccga gttcggcgcc gcggacatct caggcgccgg cgccgcgctg ggtgcggtca
    16501 cccagctggg cccgctgacc accgtgctag tgatcgccgg cgccggggcg acagccatct
    16561 gcgccgacct cggtgcccgc accatccgtg aggagatcga cgcgatggag gtgctcggca
    16621 tcgacccgat ccaccggctg gtggtgcccc gggtcgtcgc cgcgaccatc gtcgccacac
    16681 tgctcaacgg cgcggtgata accatcggcc tggtcggcgg tttcatcttc ggtgtcttca
    16741 tccagcacat ctcggccggc gcctacgtcg gcacactcac cttggtcacc ggcctacccg
    16801 aggtggtcat ctcggtgatc aagtccgcca cgttcggcct gatcgccggg ctggtcggct
    16861 gttaccgcgg cctgacgacg aagggcggcc ccaagggcgt cggcaccgcc gtcaacgaga
    16921 cgttggtcct gtgtgtcatt gcgcttttcg cggtcaacgt ggtactgacc acgatcggcg
    16981 tgcgattcgg gacggggcgc tgacgtggcc ccgacggccg agtcgtcagc ggccttggta
    17041 ctgcgcgccc gctacccgcg gaccgcggcc ggcctcaacc gctttggcgg cggcgcggcc
    17101 cgaagactcg agcagatagg cattttcgcg aggttcaccc ggatcagcgt cacgcagatc
    17161 tggtgggcgt tgcatcgcta ccgcagggag acgctccggc tcaccgccga gatcggcatg
    17221 ggtaccggcg cgatggcggt catcgggggc acggtggcga ttatcggttt cgtgacgctg
    17281 tccggcagct cgctgatcgc catccagggc ttcgcgtcgc tgggcaacat cggtgtcgag
    17341 gcgttcaccg gattctttgc cgcgctggcc aatacacgca tcgcggcccc gatcgtcgcc
    17401 ggggtcgcgc tggccgccac ggttggcgca ggggccaccg cccagttggg tgccatgcgg
    17461 atcagcgagg agatcgacgc gctggaagtc atggggatca agtcgatctc gtttctggtg
    17521 tccactcgga ttctgggcgg gctggtggtg atcgtgccgc tgtacgcgct ggccctcgac
    17581 atggcttaca cgtctggcca gatcgtcacg accgtgttct acggacagtc gaacggcacc
    17641 taccagcact acttccggac cttcctgcgc cccgaagacg tgggttggtc agtcgtgcag
    17701 gtcatcatca tcgcggtcgt ggtgatgatc acccattgct attacgggta caccgccagc
    17761 ggtggcccgg tcggcgtcgg ccaggccgtc gggcgatcga tgcgtctctc actggtctcg
    17821 gttgtggtcg tcgtcctgtt ggccgagttg gcgctctacg gcgtcgaccc gaacttcaat
    17881 ctcacggtgt aggcgcgatg acgaggctga cgacgccgac gacgctgcag aaccggcggg
    17941 tcctcctgta cgcggagggc gtggtcctgt tgttgatcgg cgcgttggtt ctcgtattgg
    18001 tgtatctgca gtttcgaggg gacttcacac ccaaggccga gctgaccctg gtggcttcgc
    18061 gggcggggct ggtcatggat cccggatcga aagtcaccta caacggggtg cagatcggcc
    18121 gggtggccgg tatctcggag atcgagtctg acggcaagcc ggcggcgaag ctcgtgctgg
    18181 acgtggaccc gaagtatctc aacctgatcc cggtcaacgt ggtggccagt atcgaggcgg
    18241 ccaccctgtt cggcaacaaa tacgtgtccc tcacctcacc ggaaaaccca caacagcaac
    18301 ggatctcgcc gcacgacgtc atcgacgtgc ggtcggtgac caccgagttc aacacattgt
    18361 tcgagacgat cacctcgatc gccgagaagg tcgacccgat cgagctgaac gccacgctgt
    18421 ccgcggtggc acaggcgctc gatgggctcg gcgcgaactt cggagactcc atcgtcgatg
    18481 gcaatcacat tctggcgcag ttgaacccac ggatgccgca gctcaatcac gacgtgcggg
    18541 ggctggcgga tctcgccgac atctatgcgc gctcgtcgcc ggatttgtgg ggctttctgc
    18601 agaacgcggt gaccacggca cgcaccttga ccaggcaaca gggcgatctg gatgccgcgt
    18661 tgttggcggc ggtgggcgcg ggcaaaaccg gcgaagacat cttcagccgg ggcgggccgt
    18721 atctggcgcg cggcgccgcc gatctggtac ccaccgctgc gctgctcgac acctacagcc
    18781 ccgagctgtt ctgcatggtg cgcaactttc acgatgccgc gccccaagtc gcgaacgccg
    18841 tcggcggcaa cggctattcg ctggcggccg ccggaacggt tttgggggcg cccaacccgt
    18901 atgtctatcc cgacaacctg ccgcgggtga acgcccacgg tggacccggt ggcaaaccgg
    18961 gctgctggca gacgatcacc cgggagttgt ggcccgcgcc ctatctggtg atggacaccg
    19021 gcgcgagcgt cgcgccgtac aaccacttcg cgcttggcga gccgatgttc ggcgaatacg
    19081 tctggggacg ccaatacggg gagaacacga tcaacccatg aaaaccaccg gcaaagccat
    19141 caaactcggc attttgtggt tggtgctgtt gatgttcacc gtgacgatcg tcgtcgtgtt
    19201 cggccaggtg cgcttcaacc acaccaccgg ctattcggcg ttgttcaccc acgtcagcgg
    19261 gctccgggcc ggacagttcg tccgggcggc gggcgttgag gtcggcaacg tctccaaggt
    19321 gacgctgatc gacggggatc aacgggtgtt ggtggatttc gccgtggaca gctcgctgcc
    19381 cctggatcaa gctacgaccg cgtcggttcg ctacctgaac ctgatcggcg accgctacct
    19441 cgagctcaag cacggcgaca gcggtcagcg tctgacaccg ggcgccacga ttccgctgga
    19501 gcacaccaaa ccggctttgg atctcgacgc gctgatcggt gggttccgcc cgcttttccg
    19561 ggcgctggac cccgacaagg tcaacagcat cgcccagtcg atcatcaccg tcttccaggg
    19621 acagggcgca accatcgacg acatcctcga ccagaccgct tcactcacct caacgttggc
    19681 cgaccgggac caggcgatcg gggagatcat caccaacttg aacaccgtgc tggccaccac
    19741 ggtcaagcac cagcaggagt tcgaccaaac aatcgacaaa ttggaggtgc tgatcaccgg
    19801 cctgaagaac agggccgatc ctttggccgc ggccgcggcc cacatcagcg acgccgcagg
    19861 caccctcgcc gacctgctgg gcgaggaccg gccgttgctg cacagcacca tcgggcacct
    19921 cgaggatgtc cagcagccga taatcggtca gctcgcgtcc ctcgacgacg tgttgcgcaa
    19981 gttgccggaa gcctaccgaa tcatcggtcg tagcggcggc acgtacggcg acttcttcaa
    20041 cttctatctg tgcgatatct cgctcaaggt caacggatta cagcccggcg gtccggttcg
    20101 caccatcaag ctcttcgggc agcccaccgg gaggtgcacg ccgcaatgag aaccctgacg
    20161 gaattcaacc ggggccggat gggtctgatg ggagtggtga tcaccctgct cgcaattggt
    20221 gtgggacaaa gctttaccag cgtgccgatg ctgttcgcca cacccaccta ctacgcggaa
    20281 ttcgtcgatt cgggcggcgt caacaccggc gacaaggtgg aaatcgcggg ggtggatgtc
    20341 ggtttggtgc gctcgctggc gatccgcggc aagcgggtcc tgatcggatt ctcgttggcc
    20401 ggcaagacaa tcgggaggca aagccgggcg gcgattcgca ccgataccct acttggccgc
    20461 aagaatatgg agatcgaacc gcacggttca gagccgttgc ggcccaacgg ttttcttcca
    20521 tctgagcaga ccaccacgcc gtaccaagtc tatgacgcgt tcgtcgatgc cacgaaggct
    20581 gcggcgggct gggacgtcga caccaccaaa cgttcgctgc aggtgctgtc ggagaccttc
    20641 gatgagactg ctccgcatct gagtgccgcc ctggacggcg tcaaggcgtt ctccgacagc
    20701 atcggtcagc gtggcgatca gctcaagcaa ctgctggcga acgccaacaa ggtcgcccgc
    20761 gtgttcggcg accgtagcga gcaggtcaac ggactgctgg tcaatgccaa taccctgctg
    20821 gcggcgttca aggaacgcag ccaggcgctg agcatgctgc tgagcaacgt ctcgggggtg
    20881 tcgacccagg tgtccggcct gctcaaggag aaccccaacg tcaatcatgt gctgcagcag
    20941 ttgaacacag tcagcgacga gctggtcaaa cgcaagcaag atctcgcgga tgtgctggta
    21001 ctgctcagca gatacaccgc agccctgacg gaggccgtcg gctccgggcc gtacttcaag
    21061 gtgatcctcg cgaatctgct gccgtaccag atacttcagc cgtgggtcga cgcggcgttc
    21121 aagaagcggg gtatcgatcc cgagcagttt tggcgctccg ccgggctgcc ggcgttccgg
    21181 ttccccgacc ccaacggcac ccggttcgcc aacggtgcgc cgccgccggc gccgccggtg
    21241 ctggagggca cgccggatca tccggcaccc gcagtgccac cgggatcgcc gtgttcctac
    21301 acaccgcccg ctgacggctt gccgcggccc ggaaatccgt tgccgtgcgc ggcgtccgat
    21361 caaggtccgt tcggcgccgt ccccggtggt ttcccggcgc cgctcgacgt cgagacgtcg
    21421 ccgccacacg ccgatgcgct gccgccggcg cccggcgtgc cgatcgcggg ccggccgggt
    21481 gagacgccgc cggccgctcc cggtacaccg acgccgctgc cggcagacgc gcctccgggc
    21541 gcacgcacgg aaccgctcgg gccggtcgcc gggccgaacc cgccgttgcc ggccccgcct
    21601 gttccggcgc cgggctgggc gcctccgggg cccggaccgc agctgccggc gccattcatc
    21661 acgcccggtg ggacgggagg cagcagtcaa tgagcaccat cttcgacatc cgaaacctcc
    21721 ggctaccgaa cgtttctcgc agggtgatag tcgccggagc gttggtggcc gttctggcca
    21781 tcgtcggggt gctcgccgga cgcgagctct atcggaaact gaccaccact tcggtggtcg
    21841 cgtatttttc tgaggcgatc gcgctgtacc cgggcgacaa agttcagatc atgggtgtgc
    21901 gggtcggttc gatcgacaag atcgaaccgg ccggtgacaa gatgcgaatc accttgcact
    21961 acagcgacaa gtacaaggtc ccggccaacg ccaccgcgtc gatcctcaac cccagcctgg
    22021 tggcatcgcg caccatccag ctgtcaccgc cctacagcga tgggcccgtg ctcacggacg
    22081 gcgcggttat cccgatcgag cgcacccagg tgcccgtcga gtgggaccac ctgcgcgatt
    22141 ccatcaacgg gatcctccgg cagctcggac cgacgcccga gcagccgaag gggccgttcg
    22201 gcgacgtcat cgaatccgcc gcggacaacc tggccggcaa gggcgaaaag gtcaacgaaa
    22261 ccctcagcag cttgtcggaa gcattgaccg cgttgaacga aggccgagga gactttgtgg
    22321 cgattacgcg cagcctggca cagttcatca gcgcgctgta ccgcaacgat caacagttcg
    22381 tcgcactcaa cggcaacctg gcccagttca ccggctggtt caccgaatcc gaccatgacg
    22441 tggccgacac actcaagcgg ctcgacgacg tgctgggcaa tgtccgaaag ttcgtaagcg
    22501 acaacaggtc aacgctggct cgcgatgtca acaacctcgc cgacgtgacc aacaccatcc
    22561 tgcaacccga accgcgggac ggcctggaaa ccgcgttaca tgtgctgccc actttcgcgg
    22621 gcaacttcaa caacatctac tccccgctgc acagctcgct ggtggggttg ttcgtgtttc
    22681 cgaacttcgc gaaccccatt cagttcatct gcagtgccat ccaggccggc agccgacggg
    22741 gctatcagga ctccgccgag ctgtgcgcgc agtatctggc gccggttctg gacgccatca
    22801 agttcaacta cctgccgttc agcgcgaact tgttcagctc ggcgacaacc ttgcccaagg
    22861 acgtggggta ctccgaggag cggttgcgtc cgccgcctgg gtacaaggac accaccgtgc
    22921 cagggatctt ctcgcgggac acaccgttct cgcacggcaa ccatgaaccg ggatgggttg
    22981 tcgcaccggg gatgcagggc acgccggtcc agccgttcac ggcgaacatg ctgaccccgg
    23041 aatcgctggc cgagctcatg ggtggtgaaa acatcgcgcc gccaccgccg ggaaacaacc
    23101 tgcccggacc gccgaacgcc tacgacgagg gcaatccgtt gccgccgccg tggtatccac
    23161 agcccgcacc gccaccgccg gccgcggctg gtaccgccgg acagacaggc ccgggtcagt
    23221 gaggcgcgga ttgctgttgc tactggcctt tttcatggtc ccagtggtgt tgacgtcgtg
    23281 cacctggcgt ggcatcgcca acgtgccgct gccggtcggt catggcgctg cctcgggtca
    23341 catgacgatc tacgtgcaga tgcccgacac gctggcgctg aacaccaaca gccgggtaag
    23401 ggtcgccgac gtatgggtcg gcaccgtgcg tgacatcagg ctgaagaact ggatcgccac
    23461 tttgacgctg gacgttgatc cggcggtgag gcttccggcg aacgcgaccg cgaaggtcgg
    23521 ccagaccagc ctgttgggca cccagcacgt cgagttggcc gcaccgccaa acgcgtcgct
    23581 gcagcccttg aagagcggcg acaccattcc tctgatgaac tcctcggcct accccacggt
    23641 cgaacggacg ctggccagcg tcgcgataat tctcaacggc ggcggcatcg cgaacctcga
    23701 cgtgattcaa accgaaatca tcaaaatcct cgacggccac gccgatcagg tccgcgaatt
    23761 cctcgggcgg ctggccacat tcaccgccgc actcaaccgc caacgcgacg acctcactcg
    23821 cgcaatcgat tcgaccaacc aattagtgac gattgtcgcg aaccgcaggg acaccctgga
    23881 tcgggtgctc accgacatcc cgccgctgat caagcatttc gccgacacgc gcgacctgtt
    23941 cgcgaacgcc accgagtccc tgggcaggtt cagtgacgtc gccaaccgga cgctggccga
    24001 tacccggccg agcctgcacc agagcttgca gtcgctgcag cgaccgttga accaactcgg
    24061 gcgcgccgcg ccatacctga tcggcgcgtt gcggctgggc ctcacagcac cgttcaacat
    24121 cgacgacgta tcgaaggttg tccgcggtga ctatgtcaac gtgtcggccg cggtcgacct
    24181 gacgctgtcc accatcgaca acgcgatcct gagcggaacg gggtactccg gggcgttgcg
    24241 cgccctcgag caggcgtggg gacgggaccc cgccaccatg atcccggatg tccgctacac
    24301 gccgaacccg aatgacgctc cgggaggacc gctggtggaa aggggcgact gagagatgtt
    24361 gactcgcttc atcaaaaccc agctggtgtt gttggtggtg gtcgcggttg ccgcggtggt
    24421 gctcctgggc tggtacttcc tgcggttacc cagcctcgtc ggcgtcggtc gatacacgct
    24481 gtatgcccaa ttg
//
LOCUS       DASSGQ010000341        11675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_814774,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000341 DASSGQ010000000
VERSION     DASSGQ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11675
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814774"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VFI55_15035"
     CDS             complement(<1..576)
                     /locus_tag="VFI55_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET7075376.1"
                     /translation="MRTRVAELLGVEFPICAFSHCRDVVAAVTNAGGFGILGAVAHSP
                     RRLESELTWIEEQTDGKPYGVDLLLPPKYVGAEQGGIDNAQARALVPEEYRAFVDDLL
                     ARYGVAEPGEGARASRGGLNVSPKGYQPLLDVAFGHDIRLIASALGPPPADLVERAHD
                     QDVLVAALAGTTHHARRHAAAGVDLIVAQGTE"
     gene            2258..8551
                     /gene="pks2"
                     /locus_tag="VFI55_15040"
     CDS             2258..8551
                     /gene="pks2"
                     /locus_tag="VFI55_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolipid-1 biosynthesis
                     phthioceranic/hydroxyphthioceranic acid synthase"
                     /protein_id="HET7075377.1"
                     /translation="MDRSGVTPVAVIGMACRLPGGINSPDLFWEALLRGDDFVIEVPA
                     DRWDVDYYYDPEPGVPGRSVCKWGSFLDSVGDFDPEFFGITEKEATAMDPQHRLLMET
                     AWEAMEHAGLTKATIAEATGVFIGLNYGDYQFVHVASGAFDGPYGNPGTISCMASGRI
                     SYALGLHGPASTVDTACSSGLFAIHQACRSLNDGESNLAFAGGVNVMMEPRRSASASA
                     GGMLSATGHCHAFDTKADGFVSGEGCVVLLLKRLTDAQRDGDRILAVVRGTAANQDGH
                     TMNIAMPSQEAQAAVYRSALAVAGVDAGTVGMVEAHGTGTPVGDPIEYSSLAEVYGVD
                     SPVVLGASKTNFGHTQAAAGALGLMKAVLAVEHGVVPKNLHFETLPDEMARIDTGLFV
                     PQEITPWPTDNGQPRRAAVSAYGISGTNVHAIVEQAPQPLSPNGAVTRPGRDGALLFP
                     LSSTSAEALRDTAGRLAEWIDTHTGEFTTPDLAYTLARRRGHRSVRTAVLATNTAELV
                     AALREVADGDLPYQEVVGQDDRGPVWVFSGQGSQWAAMGADLLANEPAFAATIAEVEP
                     LIAKESGFSVTEALTSPEKVTGIDRVQPTLFAMQVALAATMKSYGVQPGAVIGHSLGE
                     SAAAVVAGALSLEDGARVICRRSKLMTRIAGAGAMASVELPAQQVLSELMARGINDAV
                     VAVVASPQSTVIGGATQTVRDLVAAWEQREVMAREVAVDVASHSPQVDPILDDLYEAL
                     ADIEPLTPEVPYYSATSFDPREEPYCDANYWVDNLRHTVRFSAAVQAALEDGYRVFGE
                     LSPHPLLTHAVDQTARGLDMSVAALAAMRRDQPLPHGLRGLVGDLYATGAAVDFSVLY
                     PGGHLVDAPLSTWTHRSLLLKSDGQGSRSLGTNVAVHPLMGQHVRLPEEPERHVWQAD
                     IGTATLPWLGDHQVHGTAALPGAAYCEMALVAARAVFGEAAGVRDVRFEQMLLLDDET
                     EISAVATAEVPGSLLFAVATNQEGEQARRASAVLHSLEGTEAEGQPPAQDIEALLAAH
                     PSRIEGHDLRDAMDLCGIHYGPAFTGLAAAHTAEGTGTTVLAEVGLPSVIRTQQSSYG
                     VHPALLDACFQSVAAHPSVANASYGGLLLPLGVRRLRVYGPVHTARYCYSRVTAATSG
                     AVDADLDVLDKHGNVLLTVRGLQMGTGVSPSGERARLMAERLLAVEWQQRQLPEVAAA
                     ETAAWLLISTSDAADLLAAAFTDAMKLHDAECSTLSWHQGADHPATAERLRGQIEKGG
                     FTGVVVLTEPKNDSPEAEGEDGAVRGGDQVHHLVRITRELAEIPGQSPRLYVVTRNAQ
                     KVLSEDRPNLDQGGLRGLLRVIGAEQPHLHTTHVDVDEQTGTEQVVRQLLLVGPEEDE
                     TAWRNDEWYTARLCPAPLRPEERQTTIAVHESDGMRMQIRMPGDLQTMEFVAFDRVPP
                     GPGQIEIAVTASSINFADVLLAFGRYQSLDGILPQLGTDFAGVVTAVGPDVTNHKVGD
                     HVGGMSPNGCWATFVTCDARLATTLPAGLTDAQAAAVTTAHATAWYGLNDLARIKAGD
                     KVLIHSGTGGVGQAAIAIARAAGAEIYATAGSSQRRQLLRDMGIKHVYDSRSVDFADQ
                     IRRDTDGYGVDIVLNSVTGAAQLAGLKLLALGGRFIEIGKRDIYGDTKLGLFPFRRNL
                     AFWGVDLGLMSVSHPQQVSELLSTVYRLTAEGVLPMPESTHYPLAEAATAIRVMSAAE
                     HTGKLILDIPHAGRSSVVLPPEQAPVFRRDGSYIITGGLGGLGLFLAEKMAAAGAGRI
                     VLTSRSAPTQKALETVELVRSIGSDVVVECGDIAEADTAERLVASATATGLPLRGVLH
                     AAAVVEDATLTNITDELIDRDWAPKVYGAWNLHQATTDQPLDWFCSFSSAAALLGSPG
                     QGAYAAANSWLDAFTHWRHAQGLPATAIAWGAWSEIGRGADFAEGTGAAIDPDEGAYA
                     FEELLRHDRAYTGYSPLIGTPWIASFAERSKFVEMFKSAGEKRSGSSKLRAELAELPI
                     DEWPTRLRRLLSEQIGLILRRSIDVDHPLSEYGLDSLGNLELRTHIESQTGVRITSAD
                     ITTVRGLADHLFDKLAPKEEAPA"
     gene            8766..10055
                     /locus_tag="VFI55_15045"
     CDS             8766..10055
                     /locus_tag="VFI55_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HET7075378.1"
                     /translation="MQIGHLRGYVEFRDRGLDYSRAVMGSWDVPGRCDIRAMTYVINA
                     HLRRHATYHSWFEYNDAKNVVRHTLKNPRDIQFAPKEYGVLTQQEWQDHVLATPDPLQ
                     WDCFRFGIIQHEDRFALYAVVDHLHCDPMLIAGLYVEILMNYTALLEGKAPVTLPPAA
                     SYDDFCMRENHCVSSMTLESPEVRKWIEFAEANGGTLPDFPLPLGDQSIPCGGDVHVE
                     RLLGPDQTAEFEALCQQAGARFSGGLFACAALAHYELTGAETYYGLTPTDKRKSPADF
                     MTVGWFTGVVPFTVPVDPNSFEETARAAQASFDANMDLSNVPYDRVLELAPWLRKHGP
                     QFTMMSYMDAGLPPLSAIVATALDGVNATAFTDGRSPAYMYSTVFRLFDEVSIMVSYP
                     NNPVARESVTRYTEAMKSVFDRVVAGSLAGVPVRVAR"
     gene            10162..11412
                     /locus_tag="VFI55_15050"
     CDS             10162..11412
                     /locus_tag="VFI55_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7075379.1"
                     /translation="MKFVLAAHGTRGDVEPCATVGVELRRRGHEVRMAVPPNLVEFVE
                     AAGLAAVSYGPDTREQIVAVADFTHNAFKIQNPVDFVRAGKELFVEGWAGMSRTLTTL
                     ADGADLLLTGQTYHGVVANVAEYYDIPVAGLHHFPVRVNGQIGVPFLPSPAPVVRTTL
                     KAGWWLYSQITKADEDAQRRELGLPRTTVPAAKRMADGGTLEIQAYDQAVFPGLAAEW
                     TGRRPFVGALTLELGAHTDDEVASWIAAGKPPIYFGFGSTPVQSPAETIAMIADACAE
                     LGERALVYSGADDANPTPHPGHVKLVGQVNYSTILPTCRAAVHHGGAGTTAAGLRAGL
                     PTLILWDVADQFIWATQVKRLKVGSAKRLSNITRRSLVRRLGKILAPDYVARARQIAP
                     KMTKPAVGVATAADLLEQTVRVGV"
     gene            complement(11436..>11675)
                     /locus_tag="VFI55_15055"
     CDS             complement(11436..>11675)
                     /locus_tag="VFI55_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014588344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7075380.1"
                     /translation="VAEWEAALAAAPLRKISERAIDDEARRGLAANTQRTQQQIGRRL
                     PAFMNSLIRYGVGVLDWELRRAGGFSYRVYCFVKD"
BASE COUNT         1990 a         3923 c         3821 g         1941 t
ORIGIN      
        1 ctcggtgccc tgcgcgacaa tcaggtcgac tccggccgcc gcgtgccgcc gcgcgtggtg
       61 cgtggtgccg gccagggcgg ccaccagcac gtcctggtcg tgagcacgtt cgaccaggtc
      121 cgccgggggt gggcccaggg cgctggcgat cagccgaatg tcgtggccga aggccacgtc
      181 cagcagcggc tggtaaccct tgggcgagac gttgaggccg ccgcgtgacg cgcgcgcccc
      241 ctcgcccggt tccgcgacgc catagcgggc cagcaagtcg tcgacgaacg cgcggtactc
      301 ctcgggcacc agcgcccggg cctgcgcgtt gtcgatcccg ccttgctcgg cgccgacgta
      361 tttgggtggc agcagcaagt cgaccccgta gggcttgcca tcggtctgct cctcgatcca
      421 ggtcagctcg ctttccagcc gacggggact gtgcgcgacg gcgccgagga tcccgaagcc
      481 gcccgcattg gtcaccgcgg ccaccacatc gcggcagtgg ctgaacgcac atatcggaaa
      541 ctcgacgccg agcaactcgg cgaccctggt tcgcatggcc cgacgctaag agttattgat
      601 tgataagtca atcgattgac ggtggtgcgc ccatgccgga gccatgcccg taccaaccgc
      661 cgtcacaaag ggcgatcggc cgtccggccg ccagccggat atgccgtctt ctgacgggac
      721 gacaatcgct gagaaccgcc gagaacctct gtatcgccgc gcaattcgca tctgcaggcc
      781 cgaccacttt cgccgccggc gtccgggtac cggcgggaca ccgtcgcatt cagggagtca
      841 gcgattcccc gccgccttcg aggcgccgcc ctcggcgcgc ggcccaagcc atcgcatgcg
      901 tgaataccgt tggggccctg atggttacgc cgacgatgac atccgcgtca ctgacggcaa
      961 gcccgacggt caggtccccg gcccgaatcg cgcgacggaa gccgttgcgc cccagggcat
     1021 tttacgcggt gttcagcaca ggcatcccgc cacccgcgtc gcggtgcatc ggaagcatcc
     1081 cccgcatgct gcgcgaggcg tggcgttcgc ggccgcgatg ctcgctgcac gggcagccag
     1141 tgcattcccg atgacgggga cggctctgcg gccacccggt cggcgaccga aacgccccgc
     1201 cgcggcaccg ccggcctcga cgatccgacc gccgaatgcc ccgaatgccc cgaatgcccc
     1261 gaatgaatac aaacctggcg gcgagttcgt ccgcgtatag cccacgaccg gcccgaggcc
     1321 cgccggcggt cggccacccg ccgatgacca ggggaaactt ccaaatgacc aggtcacagg
     1381 gtcccacacc cgccaaagcc caccaacgcc ccaccaagcc cccaccaaag cccacgccat
     1441 cggaccgtcg gacctgttcg gacgcatatt cgagcgttcg cggatagctc gaagagcggc
     1501 cgctttccgg caactcgatc ggtatgtgcg tcaacgggtt cccagaggac gaatcgcgct
     1561 cttcccgaaa atcctcatgc cacatgacca gggacgttgc gctgctgcgc aggacaccgc
     1621 gcagcagcag acgcacgctg cgacgctggc ttcagcactc gcagcggact cgcatccaga
     1681 gggagttcga gatgtgatct tatcgatttc aacgattcac catcgcggtt tcggcaaaac
     1741 acctaatatg gccttaacga gcatcaccgt cgatgcgtag tgcgcgacgc ctattacgtg
     1801 ttcgcctggc ctttttcggg ctgggccagt atgtggtgtt catcaccatg gcgtcgcatt
     1861 cgaaccgacg ggaggaaaca cgcgacccca ataacggcgg actgtcttgg cggacagcgg
     1921 ctctggctgc actccgctac cagcacaacc ttggggtatc gtccgaaaag tatgcgactc
     1981 accgaagtaa ctagactgcc gtcagctacg cgaactctga gattgttaat tttttggacg
     2041 tccgcacgat tcttctacat gtgcgaagct aagccgctac tctgctgatc gcgccaggtt
     2101 gttccggatg gcttcgatag cgggggtaga tgggttgcta gtgctcgcta cgaagttggt
     2161 agccagcact gaaaattcag caatatcttt cgaagccctg ataagagctt gcagcagtga
     2221 taggaacttc aggacaggca gggagaggtg aaggacagtg gataggtcag gtgtgactcc
     2281 ggttgctgtc atcgggatgg cgtgccggtt gccggggggc atcaactccc ctgacctttt
     2341 ttgggaggcg ttgctacgtg gtgatgactt tgtcatagag gttccggccg accgttggga
     2401 cgtcgactac tactacgacc ccgagccggg tgtgcctggt cgttccgtgt gcaagtgggg
     2461 ctccttcctg gacagcgtgg gcgacttcga tccagagttc ttcggcatca ctgaaaaaga
     2521 agcgactgcg atggaccccc agcaccggtt gctgatggaa accgcgtggg aggccatgga
     2581 acacgccggc ctgaccaagg ccaccatcgc cgaagccacc ggtgtgttca ttggattgaa
     2641 ttacggcgac taccaattcg tgcacgtcgc cagcggtgcg ttcgatggac cgtacggaaa
     2701 ccccggcacc atttcctgca tggcatccgg gcggatctcc tacgcgctcg gactccacgg
     2761 tcccgcgtcc acggtggaca ccgcgtgctc ttccggtttg tttgctattc accaagcctg
     2821 ccgcagtctg aacgacggtg aaagcaacct tgctttcgcg ggcggggtga acgtgatgat
     2881 ggagccgcgc aggtccgcct cggcctcggc cgggggcatg ctgtcggcca ctggccactg
     2941 ccatgcgttc gacaccaaag cggacggctt cgtctccggc gagggatgcg tagttctttt
     3001 gctgaagcgc ctcacggatg cgcagcggga tggtgaccgg atcctggctg tcgtgcgtgg
     3061 cacggccgcc aaccaggacg gccacaccat gaatatcgcg atgccgtcgc aggaagcgca
     3121 ggctgccgtt taccgttcgg cgctggccgt ggctggcgtg gacgccggca ccgtcggcat
     3181 ggtcgaggcg cacggcaccg gtaccccggt cggcgatccg attgaatatt cttctctcgc
     3241 agaggtatac ggggtcgaca gccccgtcgt gctgggagcc tcgaagacga acttcggaca
     3301 cactcaggcc gccgccggtg cgctgggtct gatgaaagcg gttctcgccg tagagcatgg
     3361 cgtggttccg aagaacctgc atttcgagac cctgcccgac gagatggccc gcatcgacac
     3421 cggtcttttt gtgccgcaag agattacacc ttggccgacg gacaacggcc agccacgacg
     3481 agcggcggtg tcggcgtacg gaatatccgg cacgaacgtc cacgccatcg tcgagcaagc
     3541 cccccagcct ctttcaccca acggcgcggt aacccggccc ggccgcgacg gagcgctgct
     3601 tttcccgctg tcgtccacct cggccgaggc acttcgggac accgctgggc ggctcgctga
     3661 gtggatcgac acgcacacag gcgaattcac gacaccggac ctggcctaca cgctcgcgcg
     3721 caggcgtgga caccgctcgg tgcggacggc cgtgctggcc accaacaccg ccgaactggt
     3781 ggcggcgtta cgcgaggtcg ccgacggtga cctgccgtat caggaggttg tgggtcaaga
     3841 cgaccgcggt ccggtgtggg tgttctccgg gcagggttcc caatgggcag cgatgggcgc
     3901 cgacctgctg gccaatgagc ccgcattcgc ggcaaccatc gccgaggtgg agccgctgat
     3961 cgccaaggag tccggcttct cggtgaccga agcgctgacc tcacccgaaa aggtgaccgg
     4021 tatcgaccgg gttcagccga ctctgttcgc catgcaggtc gcgctggcag ccacgatgaa
     4081 gtcctacggg gtgcagcctg gtgcggtgat cggccattcg ctgggcgagt ccgctgccgc
     4141 cgtcgtcgcg ggggcgctat ccctcgagga cggggcgcgg gtcatctgcc gccgctcgaa
     4201 gctgatgacg cggatcgccg gtgccggcgc catggcgtcg gtggaactgc ctgcccagca
     4261 ggtcctttct gagctgatgg ctcgcggcat caacgacgcc gtagtcgcgg tggtggcctc
     4321 cccgcaatcc accgtgatcg gtggcgccac ccagaccgtt cgcgatctcg tcgccgcctg
     4381 ggagcagcgt gaggtgatgg cccgcgaggt ggccgtcgac gtggcgtcgc actccccgca
     4441 ggtcgacccg atcctggacg acctctacga ggctctggcg gacatcgagc cgctgacgcc
     4501 cgaggtgccc tactactcgg cgacgtcgtt tgacccgcgc gaagagccct actgcgacgc
     4561 caactactgg gtggacaacc tgcgtcacac ggtgcggttc tcggcggcgg tacaggcggc
     4621 actggaagac ggctaccggg tcttcggcga gctgtcaccc catccgctgc tgacccacgc
     4681 ggtcgaccag accgcccggg gtctcgacat gtcggtggcc gctctggccg ccatgcggcg
     4741 cgaccagccg ctgccccacg gtctgcgcgg cttggtcggc gacctctatg ccacgggtgc
     4801 cgcggtcgac ttctcggtcc tgtaccccgg cggccacctg gtggacgcgc cgctgtcgac
     4861 ctggactcac cggtcattgc tgctgaagag cgacggtcaa ggttcgcgct ccctgggcac
     4921 caacgtcgcg gtgcatccgc tgatgggcca gcatgtgcgc ctgccggagg agcccgagcg
     4981 tcacgtctgg caggccgaca ttggcaccgc gacgctgccc tggctgggcg atcaccaggt
     5041 ccacggcacc gccgccctcc cgggcgcggc ctactgcgag atggcgctgg tcgcggcccg
     5101 tgccgtcttc ggcgaggctg ccggggtccg cgacgtgcgc ttcgagcaga tgctcctgct
     5161 ggacgacgaa accgaaatca gcgcggtcgc cacagcggaa gtgcctggtt cgctgctgtt
     5221 cgcggtggcg accaaccagg agggcgagca ggcgcgtcgc gcctccgcgg tgctgcattc
     5281 cctggaaggc acggaagccg agggccagcc tcccgcgcag gacatcgagg ccctgctcgc
     5341 ggcccatccg agccgcatcg aaggccatga ccttcgggac gcgatggatt tgtgcggtat
     5401 tcactacggc cccgccttca ccggcctggc cgccgcgcac accgccgagg gaaccggcac
     5461 cacggtgctt gccgaggtcg ggttgcccag cgtgatccgg acgcagcaaa gcagttatgg
     5521 cgtacatccc gcgcttctcg acgcctgctt ccagtccgtg gcggctcacc ccagcgtcgc
     5581 gaacgccagc tacggcggtc tgttgttgcc gctgggtgtt cgccggctgc gggtctacgg
     5641 cccggtccac acggcccgct attgctactc gcgggtgacc gcggcgacca gcggcgccgt
     5701 cgacgcggac ctcgacgtgc tggacaagca cgggaacgtc ctgctgaccg tgcgtggcct
     5761 gcagatgggc accggtgtct cgcccagcgg cgaacgggcc cggctgatgg ccgagcggtt
     5821 gctggctgtc gaatggcagc agcggcaact gcccgaggtg gccgcagccg agaccgcggc
     5881 atggctgctg atcagcacct ccgacgccgc ggatctgctg gcggccgcct tcacggacgc
     5941 gatgaagctg cacgacgccg aatgcagcac cctgtcctgg catcagggcg ccgatcatcc
     6001 ggccactgcc gagcggttac gcggacagat cgaaaagggc gggttcaccg gcgtggtggt
     6061 actcaccgag ccgaagaacg acagcccgga agccgaaggc gaggacggcg ctgtgcgtgg
     6121 cggggaccag gtccaccacc tggtccggat cacccgcgaa ctggcggaga ttccgggcca
     6181 gtccccgcgg ctgtacgtcg tcacccggaa tgcgcagaag gtgctgtccg aggaccgtcc
     6241 caatctcgac cagggcggac tgcgggggct gctgcgggtg atcggtgccg agcaaccgca
     6301 cctgcacacc acccacgtcg acgtggacga gcagaccggc accgagcagg tggtgcgcca
     6361 gctgctgctg gtcgggccgg aggaagacga gacggcctgg cgcaacgacg agtggtacac
     6421 cgcgcgcctg tgcccggctc cgctgcgtcc cgaagagcgg caaaccacca tcgcggtcca
     6481 cgagagcgac ggcatgcgga tgcagatccg catgcccggt gacctgcaga cgatggagtt
     6541 cgtcgcgttc gaccgggtgc cgccgggccc cggacagatc gaaatcgcgg tcaccgcgtc
     6601 gagcatcaac ttcgccgatg tcctactcgc gttcggccgg taccagagcc tcgacggtat
     6661 cctgccccag ctgggcaccg atttcgccgg cgtggtgacg gccgtcggac ccgatgtgac
     6721 caaccacaag gtcggtgacc acgtaggcgg catgtcgccc aacggctgct gggccacgtt
     6781 cgtcacctgc gatgcacgcc tggccaccac gctgccggcg ggcctgaccg acgcccaggc
     6841 tgccgcggtg accaccgcgc acgccaccgc ctggtacggc ctgaacgatc tggcccgcat
     6901 caaggctggc gacaaggtcc tgatccactc cggcaccggc ggtgtcggac aggccgcgat
     6961 cgcgatcgct cgcgcggcgg gagccgagat ctacgccacc gccggcagct cacagcgtcg
     7021 acaactgttg cgggacatgg gaatcaagca tgtctacgac tcccgcagcg tcgacttcgc
     7081 cgaccagatc cgccgcgaca ccgacggtta tggcgtcgac atcgtgctca actccgtcac
     7141 cggtgccgca caactcgccg gactgaagct gttggcgctg ggcggacggt tcatcgagat
     7201 cggcaagcgc gacatctacg gggacaccaa gctgggcctc ttcccgttcc gtcgcaacct
     7261 cgccttctgg ggcgtcgacc tgggattgat gtcggtcagc cacccccagc aggtcagcga
     7321 gctgctgagc acggtgtacc ggctgaccgc cgagggcgtg ctgccgatgc cggagagcac
     7381 gcactacccg ctcgcggagg cggccacggc gattcgcgtg atgagcgcgg ccgaacacac
     7441 cggcaagctc atcctcgaca tcccgcacgc gggccgcagc agcgtggtgc ttccgcccga
     7501 gcaggcgccg gtattccggc gcgacgggtc ctacatcatc accggcggcc tcggtggcct
     7561 gggattgttc ctggccgaga agatggccgc cgccggcgcc ggccggatcg tcctcacctc
     7621 gcggtcggcg ccgacgcaaa aggcgttgga gacagtcgaa ctcgtccgtt cgatcgggtc
     7681 cgatgtggtg gtggagtgcg gcgacatcgc cgaggccgac accgccgagc ggctggtggc
     7741 atcggcaacc gccaccggac tgccgttgcg cggcgtgctg cacgcggccg cggtggtcga
     7801 agacgccacc ctgaccaaca tcacggacga gttgatcgac cgtgactggg cgcccaaggt
     7861 gtacggcgcg tggaacctgc accaggccac caccgatcag ccgttggatt ggttctgctc
     7921 gttctcctcg gcggccgcct tgctgggttc gccgggccag ggtgcctacg ccgcggccaa
     7981 cagctggctg gacgccttca cccattggcg gcacgcgcag ggcctgccgg ccaccgcgat
     8041 cgcctggggc gcctggagtg agatagggcg cggtgctgac ttcgcggaag gtacgggcgc
     8101 cgcgatcgat cccgacgagg gtgcctacgc gttcgaggag ctgctgcgcc acgaccgcgc
     8161 gtacacgggc tacagcccgc tcatcgggac accgtggatc gcgtccttcg cggaacgaag
     8221 caagttcgtc gaaatgttca agtccgccgg ggaaaagcgt tcgggttcaa gcaaactgcg
     8281 tgccgaattg gccgagctgc ccatcgacga gtggccgacc cggctgcggc gcttgctctc
     8341 ggagcagatc ggtttgatcc tgcgccgcag catcgatgtg gaccacccgc tttccgagta
     8401 cggcctcgac tcactgggga acctcgaatt gcggacccac atcgagtccc aaaccggggt
     8461 gcgcatcacg tccgccgaca tcaccaccgt tcggggtttg gcggatcacc tgttcgacaa
     8521 gttggcaccc aaagaggaag cgcctgcgtg acggacatcc ggacggcaat gtggcctata
     8581 ttgcgtgagc tgcaacagag gagggaaacg tgttcatagg tggaggctct gagcacgaca
     8641 gcctacgggt aggaaatgcc tacgactggg acccgggtgc gggctgtgtc gtgacgtggc
     8701 agccaacgcc cggctcggct gcgaaagccc gccaggcacc cgtaagcccg gtgccgccca
     8761 gctccatgca gatcggccat ctgcgtggct acgtcgagtt ccgcgaccgc gggctcgact
     8821 attcgcgggc ggtcatgggc tcctgggacg tacccggccg ctgcgatatc cgcgccatga
     8881 cgtatgtgat caatgcgcac ctgcggcgtc acgcgaccta ccacagctgg tttgaataca
     8941 acgacgccaa gaacgtcgtc cgtcacactc tgaagaaccc ccgcgatatt caattcgccc
     9001 cgaaggagta cggcgtgttg acgcagcagg agtggcagga ccacgtcctg gcgacgcccg
     9061 acccgctgca gtgggattgc ttcaggttcg gcatcattca gcacgaggac cgcttcgcgc
     9121 tgtatgcggt cgtcgaccat ctccactgcg acccgatgtt gatcgccgga ctgtatgtcg
     9181 agatcctgat gaactacacc gcgctgctgg agggcaaggc gcccgtcacg ctgccgccgg
     9241 cggccagtta tgacgatttc tgcatgcgtg agaaccactg cgtgtcgagc atgacgctgg
     9301 agtcgcccga ggtgcgcaaa tggatcgagt tcgccgaggc caacggcggg accctaccgg
     9361 atttcccgtt gccactgggt gaccaatcga ttccctgcgg tggagacgtc cacgtggagc
     9421 ggctgctggg cccggaccaa acggccgagt tcgaagccct ctgccagcag gcgggtgccc
     9481 ggttcagcgg tggcctgttc gcctgcgcgg ctctggccca ctacgaattg accggcgcgg
     9541 aaacgtatta cggtctcact cccaccgaca agcgcaagag cccggcggac ttcatgacgg
     9601 tgggttggtt caccggtgtg gtgccgttca ccgttccggt cgacccgaat tcgttcgagg
     9661 aaaccgcccg cgccgcccag gcgtcgttcg acgcgaacat ggacctgtcg aacgtgccct
     9721 acgaccgcgt cttggaattg gcgccctggc tgcggaaaca cgggccccag ttcaccatga
     9781 tgagctacat ggacgcgggg ttgcctcccc tttccgccat cgttgcgacc gccctggacg
     9841 gggtcaacgc gaccgcgttc accgacggca ggagtccggc gtacatgtac tcgacggttt
     9901 tccggctgtt cgatgaggtc tccatcatgg tgtcctaccc gaacaacccg gttgcccgcg
     9961 agtccgtgac gcgctacacg gaggcgatga aatcggtatt cgaccgggtg gttgcgggca
    10021 gcctcgcagg ggttccggtt cgcgtagcca ggtagggcca ggtaggcata gctccacttc
    10081 atccgagcag agcgtccggt gccttgaaat gcgcctgaac gcgcctgcgc ggcaccggaa
    10141 gtgaaaggct gagctgcgcc gatgaagttt gttttggcag cccacggaac tcgcggcgac
    10201 gtcgagccct gcgccaccgt gggtgtggag ctacggcgac gcggccacga agttcgaatg
    10261 gcggtgccgc ccaacctggt tgagttcgtc gaggcggccg gtctcgctgc cgtcagctac
    10321 gggcccgaca cgcgagagca aatagtcgct gtcgcggact tcacccacaa cgccttcaag
    10381 atccagaacc cggtcgactt cgtgcgtgcc ggcaaggagc ttttcgtcga gggctgggct
    10441 gggatgagcc ggacgctgac cacgctggcc gacggggccg acctgctttt gacgggccag
    10501 acctatcacg gggtggtcgc caatgtcgct gagtactacg acattccggt ggcggggctg
    10561 catcacttcc cggtacgggt caacggccag atcggcgttc catttctacc gtcaccggca
    10621 ccggtggtcc gcaccacgtt gaaggcgggt tggtggctgt attcccagat caccaaggca
    10681 gacgaggacg cgcaacgccg tgaactcggc ctgccacgga caacggttcc cgcagcgaaa
    10741 cggatggccg acggcgggac actggaaatc caggcctacg accaagcggt gtttcccgga
    10801 ctggccgcgg aatggaccgg ccggcgcccc ttcgtcggcg ccctgacgct ggagttggga
    10861 gcgcataccg acgacgaggt cgcctcgtgg atcgcggccg gcaaaccccc tatttacttc
    10921 ggttttggta gcacaccggt ccaatccccc gccgagacga tcgcaatgat cgcggacgcc
    10981 tgcgccgagc tgggcgaacg ggccctggtc tattccggcg ccgacgacgc caaccccact
    11041 ccccatcccg gccacgtgaa gctcgtcggt caggtcaact acagcacgat cttgcccacc
    11101 tgccgcgcgg ccgtccacca tggtggcgcc ggtaccaccg ccgcgggcct gcgcgccgga
    11161 ctcccgacgt tgatcctgtg ggacgtggcc gatcagttca tctgggcgac tcaggtcaag
    11221 cgactcaaag tcggctccgc caagcgtctt tcgaacatca cccgcaggtc gctggtcagg
    11281 cggctaggca agatcctcgc gccggactac gtcgcccggg cacgccagat cgccccgaag
    11341 atgaccaaac ccgctgtcgg cgtcgccacc gccgccgatc tgttggaaca gaccgtccgc
    11401 gtgggcgtgt gagacggccg ctacggcttg tcgcatcagt ccttgacgaa gcagtacacc
    11461 cgataggaaa accccccggc gcgacgcagc tcccagtcca aaacgcctac gccataacgg
    11521 atcaaggagt tcatgaacgc cggcaggcgg cggccgatct gctgctgagt ccgttgcgtg
    11581 ttcgccgcca gcccgcgcct ggcctcgtcg tcgatggccc tctcggaaat cttccgcaac
    11641 ggggcggcgg ccagcgccgc ttcccattcg gcgac
//
LOCUS       DASSGQ010000342        18512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1362286,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000342 DASSGQ010000000
VERSION     DASSGQ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18512
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362286"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            657..1460
                     /locus_tag="VFI55_15060"
     CDS             657..1460
                     /locus_tag="VFI55_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HET7075381.1"
                     /translation="MTESPSSYLVLASQRSGSTLLVESLRATGVAGEPQEFFQYLPTT
                     SQAPQPREWFAGVGDESVLSLLDPLDEGKPDLASAEIWRDYIRTVGRTPNGVWGGKLM
                     WNQTPLLLNRAAELPDRSGDGLLAAIRDVVGEEPLLVYVYRPDVVSQAVSFWRAVQTR
                     VWRGRPDPVRDARATYHAGAIAYVVTMLRDQEQGWRNWFAEADIKPMEISYPVLWRNL
                     TQVVATILEELGLDPRLAPPPVLERQADQRSDEWVDRYRADAEREGLQT"
     gene            1510..2442
                     /gene="cysD"
                     /locus_tag="VFI55_15065"
     CDS             1510..2442
                     /gene="cysD"
                     /locus_tag="VFI55_15065"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HET7075382.1"
                     /translation="MTTTVPTDTSETRRVDELRLLEAEAVHIIREVVAELERPVLLFS
                     AGKDSIVLLRLAEKAFRPLPLPFPVMHVDTGHNFPEVIDFRDRRVTGHGHKLIVASVQ
                     ESIDNGRVLDPGPGASRNRAQTRTLLDALEAGGFDAAFGGARRDEERARAKERILSFR
                     DEFGQWDPRAQRPEPWSLYNGRIKRGEQVRVFPLSNWTELDVWRYIELEDLELPPIYF
                     AHEREVFERDGILLAVSDYADPQADETSAVEWVRYRTVGDLTITGAVRSRATDIARVI
                     AEISAATVSERGETRADDRTSAAAMEDRKREGYF"
     gene            2442..4367
                     /gene="cysC"
                     /locus_tag="VFI55_15070"
     CDS             2442..4367
                     /gene="cysC"
                     /locus_tag="VFI55_15070"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013127918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HET7075383.1"
                     /translation="MTATEDTEQIEDVQSPKGITRQLLRIATAGSVDDGKSTLIGRLM
                     HDTDSLPLDHLEAVTDEDGVADLAALSDGLRAEREQGITIDVAYRFFSTESRSYILAD
                     TPGHERYTRNMFTGASNAHVAILLVDARAGVLRQTRRHARIAKLLGIKHYVATVNKID
                     LVDFDEARFAVVQDQLRQLAARLGAVDISVIPIAALHGDNVVHASDRTPWYGGPTLLE
                     YLESVELAAPQAEASRLRLPVQWVSRPTADVRRRYTGRLAAGTLSVGDPVVSLPAGTR
                     STVTALDTLDDDRAIAVAPLSVSIELADDIDVGRGDVLISGADDAVLPVLARELDATV
                     CWFADTPLRAGDRLALKQTTKTVRATVQELHSRLDPETLDELDGPVELALNDIGTVTL
                     RTSSIVVADSYTDNRDSGALILIDETTNDTVGAGTIIEAREVRPGTHPRSDIRWHPSA
                     LDRGHRWQATGQAGATIWFTGLPASGKSTIAVAVERALVESGRVAYLLDGDNLRHGLS
                     DDLGFSPGDRTENIRRVSHLTRLLADAGVVALASLVSPLKSDRETARALNDAAKLPFI
                     EVHVATSLTECEKRDPKGLYARARRGELTGLTGVDAPYEPPENPELVLDTTGASVDEL
                     AARVIALLNEVSPRRRT"
     gene            4423..4554
                     /locus_tag="VFI55_15075"
     CDS             4423..4554
                     /locus_tag="VFI55_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075384.1"
                     /translation="MVDTDTPQWRGWLERVGDDWWATLLAGVIVALAVAGALPKIPW"
     gene            4559..5677
                     /locus_tag="VFI55_15080"
     CDS             4559..5677
                     /locus_tag="VFI55_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HET7075385.1"
                     /translation="MSTTSTVSPEPDEKSAEPTFTSNRPLDYVPGILLLIAVGVLGKY
                     AQVWWNTLAKHHHWTVPDIEYVLWAIVIGLLITNTVGLHPIFRPGVLTYQFWLKVGIV
                     VLGARFVLGDIAKLGALSLVQILVDMTVAGTVIILAARAFGLSGKLGSLLAIGTSICG
                     VSAIVAAKGAIRARNSDVGYAIAAILALGAVALFVLPPLGHAIGLTDREFGLWAGLAV
                     DNTAETTATGYLFSDHAGKIAVLVKSVRNALIGFVVLGFALYWAGRGEADEIAPGVKA
                     RAAFIWDKFPKFVLGFLAVSAIATAGWLTKGQAANLANVSKWAFLLTFAGVGLTTDIR
                     QIARTGWRPLVVAIIGLTVVASVSLGIVLLTSRVFHWV"
     gene            complement(5705..8068)
                     /locus_tag="VFI55_15085"
     CDS             complement(5705..8068)
                     /locus_tag="VFI55_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET7075386.1"
                     /translation="MSNPAGNPHIPASVGDIAKRIFLGKPLITEELTNEKLSNPVALG
                     ALSPDAISSTAYGTEQILIELLPAAGLAAFTLLLPINCVILVILVLVSASYRQVVMAY
                     TRAGGSYIVARENFGPRVAQVAAAALLIDYVVTVAVQSAAGTVAVVSAVPGLGPHSLQ
                     ITVGVVLVICYANLRGLKEAGWQFALPTYFFVVMVGLTIVVGVARATLGDLPVYDPAQ
                     MPGTVPVHQGDGLVMGATILTLLRAFANGGSSLTGVEAISNTVEVFRKPQGRNARRVL
                     TAMACILGFLLAGVAYLTFVTHATPYVAGFPSVLSQVGRAVFGSGVIGNILYILVQTS
                     TAAILFTGANTSFNGFPALASFVAEDRFLPRQLMKRGYRLVFSNGIIALTAFSVVLLL
                     TTGGSVNALVPFYAIGVFTGFSMAGYGMTKHELTHREPGWRRRMAINLSAGILSTIVV
                     GIFAVAKFTEGAWLVVVVFPVLVFILIRLNREYRAEAAILETFRTDRPELVKYARHKV
                     FVFVNSVDLAVIEALRYGRGLRSDELIAVHFMVDAAHAAQLRKRWDHFELDARLRVVD
                     CPDRRITRSAQLLVAKALSEYPNTNVTVLLPRRTYAPLLGRLLHDRTADKIARAVSLV
                     PDAAATIVPYDVQTRIQEAYPERFEQRVARELDKVEAWVLRDEDKDVATYEHPERPRS
                     VARVAGLIPGQRATLEGRVSQVEDVNRGRRTLRQVVVGDESGDIGIAFNPGHGGADIQ
                     PGQLLRITGKVRQSGNRPLSMVDPAYHVVEDPARAAESGDSGQSADA"
     gene            complement(8181..9293)
                     /gene="dapC"
                     /locus_tag="VFI55_15090"
     CDS             complement(8181..9293)
                     /gene="dapC"
                     /locus_tag="VFI55_15090"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HET7075387.1"
                     /translation="MLRPAVSASLPEFPWDTLADAKALAEAHPDGIVDLSVGTPVDPV
                     APLIREALGAASSAPGYPATAGTARLREAAVAALERRFGITGLTEAAVLPVIGSKELI
                     AWLPTLLGLGAADVVVVPELAYPTYDVGARLAGARVLRADSLTQLGPQIPALFYLNSP
                     SNPTGRVLGVDHLRKVVGWARQPGREKGPVLASDECYLGLGWDAQPFSVLHPAVCDGD
                     HTGLLAIHSLSKSSSLAGYRAGFVAGDPALVAELLAVRKHAGMMVPTPVQAAMVAALE
                     DDAHERLQRERYERRRAVLLPALRAAGFTVDDSEAGLYLWATRGEACQDSVRWLAARG
                     ILVAPGEFYGPRGARHVRVALTATDERIAAAVRRLS"
     gene            complement(9321..9647)
                     /gene="fdxA"
                     /locus_tag="VFI55_15095"
     CDS             complement(9321..9647)
                     /gene="fdxA"
                     /locus_tag="VFI55_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET7075388.1"
                     /translation="MTYTIAEPCVDIKDKACIEECPVDCIYEGDRMLYIHPDECVDCG
                     ACEPVCPVEAIYYEDDVPEQWSQYTQINADFFVELGSPGGAAKVGMTENDPQVVKDLP
                     PQGEGD"
     gene            9867..9953
                     /locus_tag="VFI55_15100"
                     /pseudo
     CDS             9867..9953
                     /locus_tag="VFI55_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626310.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
     gene            9950..11977
                     /locus_tag="VFI55_15105"
     CDS             9950..11977
                     /locus_tag="VFI55_15105"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HET7075389.1"
                     /translation="MTDPYPNLLSPLDLGFTTLRNRVVMGSMHTGLEDRAHHVDRLGE
                     YFAERAHGGVGLIVTGGYSPNRSGWLLPFAAELVSLAQARRHRRITTRVHDSGAKILL
                     QILHAGRYAYHPLSVSASSIKAPINPFRPRALSSRGIATTIADVARCAQLAREAGYDG
                     VEIMGSEGYLLNQFLAPRTNKRTDSWGGTPANRRRFPVEIVRRARAAVGSDFIISYRM
                     SMADYVEDGQSWDEIVSLARELESAGVNIINSGFGWHEARVPTIVTSVPDGAFVGISD
                     AIAEQVNVPVVASNRINMPQAAEHILAETRVQLISMARPLLSDPEWVLKARTDRVAEI
                     NTCIACNQACLDHAFAKKTASCLLNPRAGRETQLVLSPARRARSVAVVGAGPAGLAAA
                     LGAAQRGHRVTLFEANDFIGGQFDLARRIPGKEEFSETIRYFATMLDKHGVVLRLGTR
                     ARAAELTCYDYVVLATGVVPRIPAIPGVDHPMVLTYAEAVTGAKPIGPTVAVIGAGGV
                     GFDVSEFLVTDRSPTLDLTRWRAEWGATDRSDARGALTTRLPAPPAREVYLLQRTKGP
                     QGKRLGKTSGWVHRASLKAKGVHQLCGMNYERITDEGLYISFGSFGADRRRLLAVDNV
                     VVCAGQEPVRDLEHALRVAGVDPHIIGGAALATEVDAKRAIKQGTELAARL"
     gene            12171..12506
                     /locus_tag="VFI55_15110"
     CDS             12171..12506
                     /locus_tag="VFI55_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophore-related protein"
                     /protein_id="HET7075390.1"
                     /translation="MVRLSLTKLAAAIGGAAFALTAGAGIASADPLDPVINTTCNYGQ
                     VIAALNATDPAAAADLNGSPVAQGYLRQFLASGPAKRQQMAAQIQAMPQAAPYFNDVL
                     SVAGSCHNY"
     gene            complement(12522..13295)
                     /locus_tag="VFI55_15115"
     CDS             complement(12522..13295)
                     /locus_tag="VFI55_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HET7075391.1"
                     /translation="MKRQIDTDRFPEWQPFVDAVQQRRPDAGTWCEAWTVRDIVIHQA
                     GNAEELARVLGAHLDGEPVGTRTFEEREGPLRALNDADLWDALHDRMATLNEVAAAGD
                     EVPADTDVAWTGRTMKVPWFAEHMREELVLHGWDITGDRDIPAALQAALDQPWMTTHS
                     VLAVGRPLLANGAKQLRAGERIEARLRVPGADDVLLIADADQTSIGLVDSEGPATLET
                     DAAARVLLLWGRRPGDPSRICSRVGPETLGRVRRLLSGY"
     gene            complement(13345..15948)
                     /locus_tag="VFI55_15120"
     CDS             complement(13345..15948)
                     /locus_tag="VFI55_15120"
                     /EC_number="2.5.1.147"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional FO biosynthesis protein CofGH"
                     /protein_id="HET7075392.1"
                     /translation="MPLTPEAPGEEPVALPRPVVPPAVAAAVPFKASASALRRVLRRA
                     RDGVALNIDEAAVAMTARGADLADLCASAARVRDAGLESAGRRGPTGRLPITYSRKVF
                     IPVTHLCRDNCHYCTFVTVPGKLRAQGAGMYMEPDEILDVARRGAELGCKEALFTLGD
                     RPEDRWPEARDWLGERGYDSTLSYVRAMAIRVLEETGLLPHLNPGVMSWSELSRLKPV
                     APSMGMMLETTSRRLFETKGLAHYGSPDKDPAVRLRALTDAGRLSIPFTTGLLVGIGE
                     TLPERADTLHAIRKSHKEFGHVQEVIVQNFRAKEHTAMAAFPDAGIEEYLATVAVARL
                     VLGPGMRIQAPPNLVSREECLALIGAGVDDWGGVSPLTPDHVNPERPWPALDELAAVT
                     AEAGYDLVQRLTAQPKYVQAGTAWIDPRVRGHVMALADPATGLARDVNPVGMPWQEPD
                     DVASSGRVDLHAAIDTEGRNTEARSDLDSAFGDWESIRDHVHELAARAPERIDTDVLA
                     ALRSAERDPAGCTDDEYLALATADGPALEAVAALADSLRRDVVGDDVTFVVNRNINFT
                     NICYTGCRFCAFAQRKGDADAYSLSTDEVADRAWEAHVEGATEVCMQGGIDPELPVTG
                     YADLVRAVKARVPSMHVHAFSPMEIASGVTKSGLSIREWLISLREAGLDTIPGTAAEI
                     LDDEVRWVLTKGKLPTSMWIEIVTTAHQVGLRSSSTMMYGHVDRPRHWVAHLNVLREI
                     QDLTGGFTEFVPLPFVHQNSPLYLAGAARPGPSHRDNRAVHALARIMLHGRISHIQTS
                     WVKLGTERTQVMLRGGANDLGGTLMEETISRMAGSEYGSSKSVAELTAIAEGIGRPAR
                     QRTTTYAPLAA"
     gene            16172..17155
                     /locus_tag="VFI55_15125"
     CDS             16172..17155
                     /locus_tag="VFI55_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014390071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HET7075393.1"
                     /translation="MSFVVAATEALAAAASDLTGIGSTLGAANAAAAASTTGVVASAA
                     DEVSAQVAALLSQHGLGYQQLGSQIADFHEQFVQALSAGANSYAAAESSATQSLASAV
                     NAPAAAMLGNPLIGSGAGAGAAGGPAATARAAVSSAVGRVETAVLGSNFSGAAGLFGP
                     NLLGGSGLLGRGAQQIGAAGQAGALLLRPTGGVSALTAASALLAPAAVTNAAVAPVAS
                     GGIGGAIEAAYLAIEPWVQYGFNLAAYAASFVVGGLAQQINFVYYLFEPLVQAGLFNT
                     IDWLQGTITFAEGLSNFWSVTTASINQFINTEINWVLGYFPPLPPLPPIFP"
     gene            complement(17167..17634)
                     /locus_tag="VFI55_15130"
     CDS             complement(17167..17634)
                     /locus_tag="VFI55_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075394.1"
                     /translation="MGYRLHTLPSRQRPDLTETKPRAASLDDSGATDPAIRFVVLTLL
                     AVDGILSALAGALLLPSYIGSIPFPISALISGLLNGALVWAAGRWAKSPRVAALPLWT
                     WLLTVAVISIGGPGDDVILGGQGLMRYSALLLIVLGVAPPVWVLWRRGRPHWP"
     gene            complement(17727..>18512)
                     /gene="mshB"
                     /locus_tag="VFI55_15135"
     CDS             complement(17727..>18512)
                     /gene="mshB"
                     /locus_tag="VFI55_15135"
                     /EC_number="3.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-
                     D-glucopyranoside deacetylase"
                     /protein_id="HET7075395.1"
                     /translation="LGEEGEVIGDRWAELTVDRADQLGGYRIGELTTALRALGVSAPI
                     YLGGAGRWRDSGMRGTPKRPRQRFIDADERETVGALVALIREQRPHVVVTYDPNGGYG
                     HPDHVHAHAVTVAAVAGAGDGESDYPGEPWTVPKFYWTVIAESAFVAGWRALDREDLL
                     PGWSIPPQAEFDFGYSDYAIDAIIEAAPDAHAAKVAALAAHATQVVVAPTGRACALSN
                     NMVLPILAEEHYVLAAGAAGQRDERGWETDLLAGLDFTAPVRR"
BASE COUNT         3123 a         6298 c         6086 g         3005 t
ORIGIN      
        1 cgtctgccat ttctgttggc gctcctcata caccgcccgt tgttcagtct agactgcgca
       61 atcgagacat tcactcggat gactacaccc atcgaccgcc aacggtgtgc cggttcgata
      121 cggtcaggat cccctgctga ctcgccgccc gtggaaggcc cgggttgagc cgacgggcat
      181 cacccggacg gcaccggaat tcttcccgga cgccccctga aatagggcgc ttgacctgga
      241 ctaattgcgt gttatggacg cggcggcgac ggtatctttc cagccatggc actccactac
      301 gtcgaccccc aatcccgatc tttcggctca cgccgcgcta gcggcgtcga atgtgacacc
      361 ccggccgtcg acgggaccac tgctggacca ctcccagcgg acagggccgg tcaccactag
      421 gccattgcgc tgtgatgatg cccacactcg ggtgaacgcg accagccgcc gcccctgagc
      481 cggcgtggaa actccccggt cggtaacggc tgtgggccga cggctatcat cgcatccggc
      541 gccggccacc tgacgatcgc gcacgagaag ccgcgcgatt ttcgcgcaat agttatcttc
      601 aggctgaagc taattcaacg tcttgcaccg gatttgcctg cggttaggct tcgttggtga
      661 cagaaagccc gtcgtcgtat ttggtgctgg cttcccagcg cagcggcagc acgctgttgg
      721 tggaatcgct gcgcgcgacc ggtgtggccg gcgagcccca ggagttcttt cagtatctgc
      781 cgaccacgag ccaggccccg caaccgcggg agtggttcgc cggggttggg gacgagtcgg
      841 tcctgagcct gttggatccg ctcgacgagg ggaagcccga cctcgcgtcg gcggagatct
      901 ggcgcgacta catccgcacg gtcggcagaa caccgaacgg cgtgtggggc ggcaagctga
      961 tgtggaacca gacaccgctg ttgttgaatc gcgccgccga actgccggat cgctccggcg
     1021 acggtctgct ggccgcgatc cgcgatgtcg tcggcgagga accgctgctt gtgtatgtct
     1081 accggcccga cgtggtgtcg caggcggtgt cgttctggcg cgcggtgcag acgcgggtat
     1141 ggcggggcag gcccgatccg gtccgggacg cgcgtgccac gtatcacgcc ggggccatcg
     1201 cctacgtggt gaccatgctg cgcgaccagg agcaggggtg gcgaaactgg ttcgccgaag
     1261 cggatatcaa gccgatggag atttcctatc cggtgttgtg gcgcaacctg actcaggtgg
     1321 tggccaccat tctggaggaa ctgggcctcg acccacgact ggcgccccct cccgtgctgg
     1381 aacgtcaagc cgaccagcgc tccgacgagt gggtggaccg ctaccgggcc gatgccgaac
     1441 gagaagggct acagacatga cccgcgtcgg cgacgatgca aaacgaagcg atgaggagga
     1501 gcgacgcaga tgaccacgac cgtgcccacg gacacctcag aaacgcggcg ggtcgacgaa
     1561 ttgcggctac tggaagccga agcggtgcac atcatccgcg aggtcgtcgc cgagctggag
     1621 cggcccgtgc tgctgttctc ggcgggcaag gactccattg tgctgcttcg cctggcggag
     1681 aaggcatttc gaccgctgcc gctgccgttc ccggtgatgc acgtcgacac tggacataac
     1741 tttcccgagg tcatcgactt ccgtgaccgc agggtgaccg gccatgggca caagctgatc
     1801 gtcgcgtcgg tgcaggagtc catcgacaat ggccgggtgc tcgaccccgg ccccggggct
     1861 tcgcgcaacc gggcgcagac ccgcacgctg ctcgacgcgc tggaggccgg tgggttcgac
     1921 gccgccttcg gcggggcccg tcgcgacgag gaacgcgcgc gcgccaagga acgcatcctg
     1981 agcttccgcg acgaattcgg ccaatgggat ccccgcgcgc agcgccccga accctggtcg
     2041 ctctacaacg gccggatcaa gaggggcgaa caggtgcggg tgttcccgct gagcaactgg
     2101 accgagctcg atgtgtggcg ctacatcgag ctggaagacc ttgagctgcc gccgatttac
     2161 ttcgctcacg agcgcgaggt gttcgaacgc gacggtatcc tgctggcggt gtcggattac
     2221 gccgatccgc aggccgacga aacgtccgcc gtggagtggg tgcgataccg caccgtgggg
     2281 gacctgacga tcaccggggc ggtgcgttcg agggccaccg acatcgcccg ggtcatcgcc
     2341 gagatctcag cggccacggt ctccgagcgc ggcgagaccc gcgccgacga ccgcacctcg
     2401 gccgccgcga tggaagaccg caagcgagag ggctacttct gatgaccgcg accgaagaca
     2461 cagagcagat cgaagacgtc cagtctccca aggggataac gcgtcagctg ctgcgcatcg
     2521 ccaccgcggg ttcggtggac gacggcaaaa gcactctgat cggccggctg atgcacgaca
     2581 ccgacagcct gccactggac cacctggaag ccgtcaccga cgaagacggt gtcgcggacc
     2641 tcgcggcact gtccgacggt ctgcgtgccg aacgcgaaca gggcatcacc atcgacgtcg
     2701 cgtaccgctt cttttccacc gaaagccgta gctacatcct cgccgacact cccggccacg
     2761 agcgctacac ccgcaacatg ttcaccggcg cctccaacgc gcacgtggcc atcctgctgg
     2821 tcgacgcccg cgccggcgtg ttgcggcaaa cccgcaggca tgcccgcatc gcaaagctgt
     2881 tgggcatcaa gcattatgtg gcaacggtga acaagatcga cctggtcgac ttcgatgagg
     2941 ccaggttcgc cgtggtgcaa gaccagctgc gccagttggc ggcacgcctt ggcgcggtgg
     3001 acatctcggt gatcccgatc gcggccttgc acggggacaa tgtcgtgcac gcttccgacc
     3061 gcacgccgtg gtatggcggc cccacactgc tggagtatct ggaaagcgtc gagctggccg
     3121 cgccgcaggc cgaagcatcg cggctgcgcc tgccggtgca gtgggtgtcg cggccgaccg
     3181 ccgatgtgcg ccgccgctac accgggcggc ttgcggcggg aacactcagc gtcggcgatc
     3241 cggtggtgtc gctgcccgcc ggcacccgct cgacggtgac cgcgctggac acgctcgatg
     3301 acgaccgcgc gatagccgtt gcgccgctgt cggtttcgat tgagctggcc gacgacatcg
     3361 atgtcggtcg cggcgacgtg ctgatcagtg gcgccgacga cgcggtgttg ccggtgctgg
     3421 cccgagagct ggacgcgacc gtgtgctggt tcgccgacac gccgctgcgg gcaggtgacc
     3481 ggttggcgct caaacagacc accaagacgg tccgggcgac agtgcaggag ctgcactcgc
     3541 ggctggatcc ggagacgctc gacgaactcg acgggccagt cgagctggcg ctcaacgaca
     3601 tcggcaccgt gacgctgcgg accagctcga tcgtggtcgc cgacagctac accgacaacc
     3661 gcgacagcgg cgcactcatc ctgatcgacg aaaccaccaa cgacaccgtc ggcgccggca
     3721 cgatcatcga ggcgcgggag gtcaggccgg gcacgcaccc gcgctccgac atccgctggc
     3781 atccgtcggc gctggaccgt ggccaccgct ggcaggccac cgggcaggcg ggcgcgacga
     3841 tctggttcac cgggctgccc gcatccggca agtcgacgat cgcggtggcc gtggagcgcg
     3901 cgcttgtcga atcggggcgc gtggcctacc tgctcgacgg ggacaacttg cgtcacggcc
     3961 tttccgacga cctgggcttc tcgcccggcg accgcaccga aaacatcagg cgagtgagcc
     4021 atttgacccg actgctggcc gacgcgggcg tggtcgcgct ggcgtcgctg gtgtcgccgc
     4081 tcaaatcgga ccgcgagacc gcacgcgccc tcaacgacgc cgccaaactg ccgttcatcg
     4141 aggtccacgt cgccacgtcg ctgaccgaat gcgaaaagcg ggatcccaag ggcctgtatg
     4201 cgcgtgcccg ccgcggcgag ctcaccgggc tgaccggggt cgacgcgccc tacgagccac
     4261 ccgagaatcc ggaactggtg ctcgacacca ccggcgccag tgtcgacgag ctcgccgccc
     4321 gcgtcattgc tttgctcaac gaggtcagcc cgcgccggcg gacctaaatt cagcgcgagc
     4381 taatcacttg gtcagcgggg tcgagcgggc caccatggcg gggtggttga tacggacacg
     4441 ccgcaatggc ggggttggct ggagcgggtt ggggacgact ggtgggcgac gctgctggcc
     4501 ggcgtgatcg tcgcgttggc ggtcgccggt gcgctcccca agattccctg gtgatgcagt
     4561 gagcacaacc agcacagtca gcccggaacc agacgaaaag tcggccgagc ccaccttcac
     4621 cagcaatcgc cccctcgact atgtgccggg tattctgctg ctgatcgcgg tgggggtgct
     4681 gggcaagtac gcccaggtct ggtggaacac actggccaaa caccaccact ggaccgtgcc
     4741 ggacatcgaa tacgtcttgt gggccatcgt gattgggctg ctcatcacca acaccgttgg
     4801 gctgcacccg atctttcggc cgggcgtgct gacctaccag ttctggctga aagtcggcat
     4861 cgtggtgctg ggagcccgct tcgtactcgg cgatatcgcc aaactcggcg cgctgagcct
     4921 ggtgcagatc ttggtcgaca tgactgtcgc gggaaccgtc atcatcctcg ccgcccgtgc
     4981 attcgggctg tccggcaagc tgggctcgtt gctggcgata ggaacctcga tctgcggggt
     5041 gtcggccatc gtggctgcca agggcgcgat ccgggcccgc aactccgatg tcggctacgc
     5101 catcgcggcc atcctggcgt tgggcgcggt ggcactgttc gtgttgccac cactgggaca
     5161 cgctatcggc ctcaccgacc gggaattcgg gctgtgggcc ggcctggcgg tggacaacac
     5221 cgccgaaacc accgccaccg gctacctgtt ttccgatcac gccggcaaga tcgccgtgct
     5281 ggtcaagtcc gtccgcaacg cgctgatcgg attcgtcgtg ctgggattcg cgttgtattg
     5341 ggccggtcgc ggcgaagccg acgagatcgc gcccggcgtg aaagccagag cggcgttcat
     5401 ctgggacaaa ttcccgaaat tcgtgctcgg cttcctagcg gtctcggcga tcgcgaccgc
     5461 gggctggctg accaagggcc aggcggcgaa cctggccaac gtgtcgaaat gggcgttcct
     5521 gctgaccttc gccggtgtcg ggttgaccac cgacatccgc caaatcgcgc gcaccgggtg
     5581 gcgtccgctc gttgtcgcga tcatcgggct caccgtcgtc gcatcggtct cgctgggcat
     5641 cgtcctgctc acctctcgcg tgttccattg ggtctgaaca cagagcagga tctgcgtcgc
     5701 gaagctacgc gtcggcgctc tgcccggagt ctccggattc ggccgcgcgc gcggggtctt
     5761 cgaccacgtg gtatgccggg tcgaccatcg acagcggccg gttgccgctt tggcggacct
     5821 tgccggtgat ccgcaacaac tgaccgggct ggatgtcggc gcctccgtgc ccgggattga
     5881 acgcgatgcc gatgtccccg ctttcgtcgc cgacgacgac ctgtcgcagc gttcgccggc
     5941 cccggttgac gtcctcgacc tgactgactc gcccctccag ggtggcgcgc tgtcccggga
     6001 tcaggccggc caccctggcc accgaccgcg gccgctcggg gtgttcgtag gttgcgacgt
     6061 ccttgtcctc gtcgcgtaac acccacgctt cgaccttgtc cagttcgcgc gcgacacgct
     6121 gctcgaagcg ctccgggtag gcctcctgga tccgggtttg cacgtcgtag gggacgatcg
     6181 tcgccgcggc gtccgggacc aggctgaccg cccgggcgat cttgtccgcg gtgcggtcgt
     6241 gtaacaggcg tccgagcagc ggcgcataag tccgtcgcgg caacagcacc gtcacgttgg
     6301 tatttggata ttcgctgagc gccttggcga ccagcagctg cgcggaccgg gtgatccgcc
     6361 gatccggaca gtccaccacc cgaagccggg cgtcgagttc gaagtgatcc caacgtttgc
     6421 gcaactgcgc ggcgtgggcc gcatcgacca tgaagtgcac cgcgatgagc tcgtcggacc
     6481 gcaggcccct gccgtagcgc agcgcctcga tgaccgcgag gtcaaccgag ttcacaaaca
     6541 cgaacacctt gtgcctggcg tacttcacca actcgggccg gtcggtgcgg aacgtctcga
     6601 gaatggccgc ctcggcgcga tattcgcggt tgagccggat caggatgaac accagcacgg
     6661 ggaacacgac gaccaccagc caggcgccct cggtgaattt cgcgaccgcg aagatgccga
     6721 ccacgatggt cgacaggatc ccggcggaga ggttgatcgc cattcggcgc cgccaacccg
     6781 gttcccgatg ggtgagttcg tgtttggtca tcccataacc ggccatcgag aatccggtga
     6841 acacaccgat cgcatagaac gggaccagcg cgttgaccga gccgccggtc gtcagcagca
     6901 ataccaccga aaacgccgtg agcgcgatga tgccgttcga aaaaaccagg cggtaaccgc
     6961 gtttcatcag ctgccggggc aggaagcggt cttcggcgac aaaactggcc agcgccggga
     7021 atccgttgaa gctggtgttc gcgccggtga acaagatggc ggccgttgac gtctggacga
     7081 ggatgtagag gatatttccg attaccccac tgccgaagac agcgcggcca acctgcgata
     7141 acaccgaagg gaatccagcg acatacggcg tggcgtgggt gacaaaagtg agataggcaa
     7201 caccggccag caagaacccg aggatgcaag ccatggcggt gagcactcga cgtgcgttgc
     7261 ggccctgcgg ctttcgaaag acctcgacgg tattagagat cgcctcgacc cccgtcagcg
     7321 atgaaccgcc gttggcgaac gcgcgcaaca aagtcaggat ggtcgcgccc atcactaagc
     7381 catccccctg atgaactggc acagttccgg gcatctgcgc gggatcgtaa accggtagat
     7441 cgccgagcgt tgcgcgcgca acgcctacaa cgatcgtcag gccgaccatg acgacaaaga
     7501 agtaggtcgg caacgcgaac tgccatcccg cttctttcaa tccgcgcagg ttcgcgtagc
     7561 agatgacaag caccacgccg acggtgatct gcaggctgtg cggacctaat ccgggaaccg
     7621 ccgacaccac cgcgaccgtc ccggccgccg actgaaccgc cacggtgacc acatagtcga
     7681 ttagcagcgc cgcggcggcg acctgcgcca cccggggtcc gaaattctcc cgcgcgacga
     7741 tgtaggaccc acccgcccgg gtgtaggcca tgacaacctg ccggtacgac gcggacacca
     7801 acaccagaat caccagaatc acgcagttga tgggaagcaa cagcgtaaac gccgccaacc
     7861 ctgcagccgg cagtaattcg atcaggatct gctcggtgcc gtaggcggtc gacgagatcg
     7921 catcaggcga aagcgcacct agcgcaacgg ggttggacag cttctcattc gtcagttcct
     7981 cggtgatcag cggctttccc aggaagatgc gcttggctat gtctccaacc gacgcaggga
     8041 tgtgcggatt gcccgccgga ttgctcaccg aacttgtcac gacacattcc ttacccgaga
     8101 gtggagccct taggaagcgt ttgcctaaca actaccgcag cgttgggctg ttcgcctcac
     8161 gagcgtgcca ggcgcccagg ctaactcagc cgccgcaccg cggcagcgat gcgctcgtcg
     8221 gtggcagtca gcgcaacgcg cacatgccgg gcgccgcgcg ggccgtagaa ctcgccgggc
     8281 gcgaccagga tgccgcgggc ggccagccac ctgacgctgt cctgacacgc ctcgccgcgg
     8341 gtggcccaca gatacaggcc ggcttcggaa tcgtcgacgg taaaacccgc cgcgcgcagt
     8401 gccggcaaca gaacggcccg ccgcctttca taccgctcgc gctgcagccg ttcgtgagcg
     8461 tcgtcctcga gtgccgcgac catggcggcc tgcaccggcg tcggcaccat catcccggcg
     8521 tgtttgcgca cggccagcag ctcggcgacc agggccggat cgccggcgac gaatccggcc
     8581 cggtaacccg ccagcgacga gctcttcgac agcgagtgga tcgcgagcag cccggtatgg
     8641 tcgccgtcgc acaccgcggg atgcagcacc gagaacggct gggcgtccca acccaaaccc
     8701 aggtaacact cgtcggaggc cagcaccggg cccttttcgc gtccaggttg ccgcgcccag
     8761 ccgaccacct tgcgcaggtg gtcgacgcct agcacacggc cggtcgggtt gctcggagaa
     8821 ttcaggtaga aaagcgccgg gatttgcggc cccagctggg tcagcgaatc cgcgcgcagc
     8881 acccgggctc cggccagccg ggcccccacg tcatacgtcg gatacgccag ctccgggacc
     8941 acgaccacat cggcggcccc caggcccaac agcgtgggca gccacgcgat gagttctttg
     9001 ctgccgataa ccggcagcac ggctgcttcg gtcaggccgg tgatgccgaa acggcgttcc
     9061 aacgcggcca ccgccgcttc acgcagtcgc gccgtgcccg cggttgccgg gtaccccggc
     9121 gcggagctgg cggcccccag cgcctcgcgg atcagtggcg cgaccgggtc gaccggggtg
     9181 ccaacggaca ggtcgacgat tccgtccgga tgggcctccg ccagcgcctt cgcgtccgcc
     9241 aaggtgtccc agggaaactc cggcagggac gccgaaaccg ccggccggag cacaccgggc
     9301 tcacgcagca cgaggcccgc tcagtcgcct tcgccctgcg gcggcagatc cttgaccacc
     9361 tgcgggtcgt tctcggtcat accgactttc gccgcgccgc cgggcgaccc cagctcaacg
     9421 aagaagtcgg cgttgatctg cgtgtactgg ctccactgtt cgggcacatc gtcttcgtag
     9481 tagatcgcct cgacggggca gaccggctca caggcgccgc agtcgacgca ctcgtcggga
     9541 tggatgtaga gcatccggtc gccctcgtag atgcagtcga ccggacactc ctcgatgcat
     9601 gccttgtcct tgatgtcgac gcagggttcg gcgatcgtgt aggtcacaga cgtctcctca
     9661 aggtctcctc caggtaattg cgtggtgggc tgctgttcta ctcggcgcgg tgcttcgacc
     9721 gtcgccgaga aaggctctcg gttactgata ctagacgttg cacatacacg tcaaccaccg
     9781 ggcacaccat caaaccgcgc ccccgttatg cacccagttg catagtcgcc tgaccgaggc
     9841 atttgagcgg cgtttaagct gcccgcgtgg tgctgcgcgg aggcatccgg gccgaggaga
     9901 gcgccatcga ctggctcgac gaggtcgcca acgcgctgca ggagtcaccg tgaccgatcc
     9961 gtacccaaat ctcctgtcgc cattagacct tggcttcacc acgttgcgca atcgagtggt
    10021 catggggtcg atgcacaccg gcctggaaga ccgggcccac catgtcgatc ggctcggcga
    10081 gtacttcgcc gaacgcgcgc acggaggcgt cgggttgatc gtcaccggtg gctactcccc
    10141 caaccgcagc ggctggctgc tgccgttcgc cgcggagctg gtctccctcg cccaagcccg
    10201 ccggcaccgt cgtatcacca cacgggttca cgactcgggc gccaagatcc tgctgcagat
    10261 cctgcacgcg ggacgctatg cgtaccaccc gctttcggtc agtgcatcgt caatcaaggc
    10321 gccgatcaac ccgttccggc cgcgcgcact gtcgtctcgc ggcatcgcga cgaccatcgc
    10381 cgacgtcgcg cggtgcgccc aactggcgcg agaggccggc tacgacggcg tcgaaatcat
    10441 gggcagcgaa gggtatttgc tcaaccagtt cctcgcaccg cgcaccaaca agcgcaccga
    10501 ttcctggggc ggcacacccg ccaaccgccg tcgattcccg gtcgagatcg tgcgtcgcgc
    10561 ccgtgccgcg gtgggatcag atttcatcat cagctaccgg atgtcgatgg ccgactatgt
    10621 cgaggatggc cagagctggg acgaaatcgt ttcgctggca agggaattag aatccgctgg
    10681 agtaaacatc atcaactccg gattcggatg gcacgaggca cgggtgccca cgatcgtcac
    10741 ctcggtgccc gacggcgcct tcgtcggcat cagcgacgcc atcgccgaac aggtgaacgt
    10801 cccggtggtg gcgtccaacc ggatcaacat gccgcaggcc gccgaacaca tcctggccga
    10861 aacgcgcgtg cagctcattt cgatggcccg gccgctgctg agcgacccgg agtgggtgct
    10921 caaagcgcgg accgaccgcg tcgctgagat caacacctgc atcgcctgca atcaggcttg
    10981 cctggatcac gcgttcgcca agaagacggc ctcgtgcctg ctcaatccgc gagccgggcg
    11041 cgagacgcag ctggtgttgt cgccggcgcg gcgcgcccgc tccgtggcgg tcgtcggtgc
    11101 gggcccggcc ggactggccg cggcgctcgg cgcggcccag cgcggccacc gcgtgaccct
    11161 gttcgaggcc aacgacttca tcggcggcca gttcgacctg gcccggcgca ttcccggtaa
    11221 ggaggagttc agcgagacca tccgctactt cgccacgatg ctcgacaagc acggcgtcgt
    11281 gctgcggctg ggcaccaggg cccgcgccgc ggagctgacc tgctacgact acgtcgtcct
    11341 ggccaccggc gtcgtacccc gcatcccggc gattccgggc gtcgatcacc cgatggtgtt
    11401 gacctacgcc gaggcggtca cgggcgcgaa gccgatcggc cccaccgtgg cggtgatcgg
    11461 cgccggcggc gtcggattcg acgtcagcga attcctggtc accgatcgct cgcccaccct
    11521 cgacctgacc cggtggcgag ccgagtgggg cgccaccgat cggtcggacg cgcgcggcgc
    11581 gctgaccacc cgtttgcccg cgccgcccgc ccgtgaggtc tacctactgc aacgcaccaa
    11641 ggggccgcag ggtaagcggc tgggcaagac cagcggatgg gtacaccggg cgtctctgaa
    11701 agccaaagga gttcatcagc tctgcggaat gaactacgag cggatcaccg acgagggcct
    11761 gtacatcagc ttcggcagct tcggcgcgga ccgccgccgg ctgcttgcgg tggacaacgt
    11821 ggtggtctgc gccggccaag agccggtgcg cgacttggag cacgccctgc gggtcgctgg
    11881 tgtcgacccc cacatcatcg gcggcgcggc gctggcaacc gaggttgacg caaagcgcgc
    11941 catcaaacaa ggcacggagc tggcggcccg gctgtagcgc gcgttttacc gagtgcgatc
    12001 cgcgccaaaa cgtcgctgac ctgcggatag ttatacttag aggttgctat gcgattactg
    12061 ttgggatgga agaaaactca cgggggctgt aatctttccg aagacggcaa ccgttgggct
    12121 gatgtcgtcg ccgtttttga cggtgcattg aatggggaag gtgtccacaa atggtaaggc
    12181 tgtcgttgac caaattagcc gctgcgattg gcggtgcggc attcgcgttg acggctggcg
    12241 ccgggatcgc atcggcggat ccgctggatc cggttatcaa caccacctgc aactacgggc
    12301 aggtgatagc cgccctcaat gcgacggatc cggcggctgc cgcagatttg aatggatcgc
    12361 cggtggcgca gggttacctg cgtcagttcc tcgcgtcggg gccggccaag cgccagcaga
    12421 tggcagcgca gatccaggcc atgccgcagg ctgcaccgta cttcaatgac gtcctttcgg
    12481 tggcgggcag ctgccacaac tactgagcca gcgcaccgac gtcagtaacc gctgagcagt
    12541 cgccgcaccc ggcctagcgt ctcgggtccg acgcggctgc agatccgcga cgggtccccg
    12601 gggcgacgac cccacagcag caacacccgc gcggcggcat cggtttccag tgtcgccggg
    12661 ccctcggagt cgaccagacc gatggaggtc tggtcggcgt cggcgatgag gagaacgtcg
    12721 tcggcgccgg gaactctgag ccgggcctcg atgcgttcgc ccgcccgtaa ttgcttggcc
    12781 ccgttggcca gcagcgggcg tcccacggcc agcacgctgt gcgttgtcat ccacggttgg
    12841 tccagcgccg cttggagggc ggcggggata tcgcggtctc cagtgatgtc ccaaccgtgc
    12901 agaacgagct cttcgcgcat gtgctcggcg aaccacggca ctttcatggt gcgaccggtc
    12961 cacgccacat cggtgtcggc ggggacctca tcgcccgccg ctgccacctc gttcagggtg
    13021 gccatccggt cgtgtagcgc gtcccacaaa tcggcgtcgt tgagggcacg aagtggaccc
    13081 tcgcgctcct cgaaggtgcg ggtgccgacc ggttcgccgt ccaggtgtgc ccccagcacc
    13141 cgggccagct cctcggcatt gccggcttga tggatcacga tgtcacgtac cgtccaggct
    13201 tcacaccagg tccccgcatc aggtcggcgc tgctgcaccg cgtccacaaa gggctgccat
    13261 tcggggaacc ggtcggtatc gatctgtcgc ttcacggctt cgggtatacc ccgcgtcgca
    13321 ggaggccaag ccaagcaatc gcagctacgc cgccagcggt gcgtaagtgg tggtgcgctg
    13381 gcgcgccggg cggccgatcc cctcggcgat cgccgtcagc tccgccacgg acttcgacga
    13441 cccgtattcc gaaccggcca tccgtgagat ggtctcctcc atcagcgtgc cgccgaggtc
    13501 gttggcgcca ccgcggagca tcacctgcgt gcgctcggtg ccgagcttga cccagctggt
    13561 ctggatgtga gagatgcggc cgtgcaacat gatccgcgcc agggcatgca ccgcccggtt
    13621 gtcgcgatgg ctgggtccgg gccgcgccgc tccggccaga tacaacggcg aattctggtg
    13681 cacgaacggc aacggcacga attcggtgaa accgcctgta aggtcctgga tttcgcgtag
    13741 cacgttgagg tgggcgaccc aatgccgtgg cctgtcgacg tgtccgtaca tcatcgtcga
    13801 cgatgaccgc agacccacct ggtgggcggt ggtaacgatc tcgatccaca tcgaggtcgg
    13861 caacttgccc ttggtcagca cccagcgcac ttcgtcgtcc agaatctcgg ctgcggtgcc
    13921 ggggatggtg tccaggccgg cctcacgcag gctgatcaac cattcgcgaa tgctcaaccc
    13981 gctcttggtc acaccgctgg cgatctccat cggcgaaaac gcgtgcacgt gcatcgacgg
    14041 caccctcgcc ttgacggcgc gcaccagatc ggcgtagccg gtgaccggga gctcgggatc
    14101 gatcccgccc tgcatgcaca cctcggtcgc gccttcgacg tgcgcctccc acgcccggtc
    14161 ggccacctcg tcggtggaca acgaatacgc gtcggcgtcg cccttgcgct gcgcgaaagc
    14221 gcagaaccgg catcccgtgt aacagatgtt ggtgaagttg atgtttcggt tcaccacgaa
    14281 ggtcacgtca tcaccgacga cgtcgcgtcg caacgaatcc gccagcgcgg caaccgcttc
    14341 caatgcgggg ccatcggcgg tcgccaacgc cagatactcg tcgtcggtgc agccggcggg
    14401 atcgcgttcg gccgaccgca gcgcggcgag cacgtcggtg tcgatgcgct cgggcgcgcg
    14461 agccgccagc tcgtgcacgt ggtcacggat cgattcccaa tccccgaagg cactgtcgag
    14521 gtcgctgcgg gcttcggtgt tgcgaccctc ggtgtcgatc gcggcgtgca ggtccacccg
    14581 tccggaggac gccacgtcgt cgggctcctg ccagggcatg cccaccggat tgacgtcccg
    14641 ggccagaccg gtcgccggat cggccagcgc catcacgtgg ccccgcaccc gcgggtcgat
    14701 ccacgccgtg ccggcctgta cgtacttggg ctgcgccgtc aaccgctgca ccaggtcata
    14761 tccggcttcg gcagtgacgg cggccaactc gtcgagggcg ggccagggcc gttcggggtt
    14821 gacgtggtcg ggtgtcagcg gtgaaacacc accccagtcg tcgactccgg cgccgatcag
    14881 cgccagacac tcctcgcgcg acaccaagtt gggcggcgcc tggatacgca tgcccggccc
    14941 gagcaccagc cgcgcgaccg ccaccgtcgc caggtactcc tcgatcccgg cgtcggggaa
    15001 agcggccatc gcggtgtgtt ccttggcccg gaaattctgc acgatcactt cctggacgtg
    15061 tccgaactcc ttgtgcgact tgcgaatcgc gtgcagggta tcggcgcgtt cgggcagcgt
    15121 ctcgccgatg ccgaccagca gaccggtggt gaacggaatc gacaaccggc ccgcgtcggt
    15181 gagcgcgcgc agccggaccg ccggatcctt gtccgggctg ccgtagtgcg caagcccttt
    15241 cgtctcgaac agccgccgcg acgtcgtctc cagcatcatg cccatcgacg gcgccaccgg
    15301 cttgagccgc gacagctccg accagctcat cacgcccggg ttcaggtgcg gcagcagccc
    15361 ggtctcctcg agcacccgga tggccatcgc ccgcacgtag gagagcgtcg agtcgtagcc
    15421 ccgttcgccg agccagtcgc gcgcctcggg ccagcggtcc tcgggccggt ctcccagagt
    15481 gaatagcgct tccttgcaac cgagttcggc cccacggcgg gcgacgtcga ggatctcgtc
    15541 gggctccatg tacatcccgg cgccctgggc ccgcagcttg ccgggcacgg tgacaaaggt
    15601 gcagtagtgg cagttgtcgc ggcacagatg tgtgaccggg atgaacacct tgcgcgaata
    15661 ggtgatcggc aaccgtccgg tagggccgcg ccgcccggcc gactccaggc ccgcgtcgcg
    15721 cacccgcgcg gcgctcgcgc acaaatcggc caagtccgcc ccgcgcgccg tcatcgcgac
    15781 ggcggcctcg tcgatgttga gcgcaacgcc atcccgggcc cgccgcaaga cccgccgcag
    15841 cgccgacgcg ctggccttga acgggaccgc ggccgcgacc gcaggcggaa cgacaggcct
    15901 cggtagagcg acgggctcct cgcccggagc ttccggtgtc aggggcacgt tggtgtaact
    15961 tccccacgat cgaatttcat ccgcgcgccc cccaattttg caaacctggg cgacggtgcc
    16021 atatgcgcaa cagtagccgc aggccgctgc cgcgccgcgg tcgccgtccc tgccccgggg
    16081 ccacgcgcag aatgctttgt cagtttggcg gacttcccgc tattgagctg cgatcgcgat
    16141 accatccgac gtagcccagg gaaggtgcca gatgtctttc gtagtcgcgg ccaccgaggc
    16201 gctggctgcg gcggcttcgg atctgaccgg tatcggttcg acgctgggcg cggccaatgc
    16261 ggccgcggcg gcgtcgacaa ccggagtggt cgcgtcggcc gccgatgaag tgtcggcaca
    16321 ggtcgccgcg ttgctttccc agcacgggct ggggtatcag caactgggca gccagattgc
    16381 ggacttccac gagcaatttg tgcaggcctt gagcgcgggc gcgaacagct acgcggcggc
    16441 cgagtcgagc gccacacaga gcctggcgag tgccgttaat gcgcccgccg cggcgatgct
    16501 cgggaatccg ctgatcggaa gcggcgccgg tgcgggggcg gcgggcggtc ccgcggccac
    16561 ggcccgcgcc gccgtctcca gcgccgtggg ccgcgtggag accgccgtac tgggctccaa
    16621 tttctcgggc gccgccgggc tgttcggccc caacctgctg ggcggctccg gtctgctcgg
    16681 gcggggcgcc cagcaaatcg gggccgccgg tcaagccggc gcgctgttgc tgcggcccac
    16741 cggcggagtc agtgccctga ccgcggccag tgcgctgctt gcgccggccg cggtaaccaa
    16801 cgccgcggtg gcaccggtgg catccggggg tatcggcggc gcgatcgagg ccgcctacct
    16861 cgcgatcgag ccgtgggtgc agtacggctt caacctcgcc gcctacgcgg ccagctttgt
    16921 ggtcggtggg ctggctcaac agatcaactt cgtctactac cttttcgagc ccttggtgca
    16981 ggccgggctg ttcaacacga tcgactggct gcagggcacg atcacgttcg ccgaagggct
    17041 gagcaacttc tggtcggtga ccacggcatc gatcaaccag ttcatcaaca ccgagatcaa
    17101 ctgggtgctc ggctacttcc cgccgctccc gccgcttccg ccgatcttcc cgtaacccgg
    17161 tcccagtcat ggccagtggg ggcggccgcg ccgccacaac acccacaccg gcggtgccac
    17221 ccccagcaca atcagcagca gagccgagta gcgcatcaac ccttggccgc ccaagatgac
    17281 gtcgtcgccg ggtccgccaa tgctgatcac cgcgaccgtg agaagccagg tccacagcgg
    17341 cagggcggcc acccgcggag atttcgccca tcgccccgcg gcccacacca atgcaccgtt
    17401 gagcaatcca ctgatcagtg cgctgatggg aaacgggatc gagccgatat aggaaggcag
    17461 cagcagggcg ccggccaggg ccgacagaat cccgtcgacg gccaacagtg tcaagacaac
    17521 gaaacgtatc gcggggtccg tggcgccgct gtcgtcgaga gacgcggcgc gcggcttggt
    17581 ttcggtcagg tcgggacgct gtcgacttgg gagagtatgg agccgataac ccactgcagg
    17641 ctgtccagcc ggtcttgcca gtgctgcagg ttagggtcca ggtcggggtc catgcgaatt
    17701 ccttccgtgg ctgcctgtgt ggcagcctac cgcctcacgg gcgcggtgaa atcaagtccc
    17761 gcaagcagat ctgtctccca gccgcgctcg tcacgctgcc ccgcggcgcc ggccgccagc
    17821 acgtagtgct cttccgcgag gatcggcagc accatgttgt tcgacaacgc gcaggcgcgg
    17881 ccggtggggg cgacgacaac ctgggtggcg tgcgcggcca atgccgccac cttggcggcg
    17941 tgcgcatccg gggcggcttc gatgatggcg tcgattgcgt agtccgaata cccgaagtcg
    18001 aactccgctt gcggcgggat cgaccacccg ggaagcagat cctcgcgatc cagcgcgcgc
    18061 caacccgcca cgaacgcgct ttcggcgata accgtccagt agaacttcgg caccgtccac
    18121 ggctcgcccg ggtagtccga ctccccgtca cccgcccccg ccaccgcggc caccgtcacg
    18181 gcatgggcgt ggacgtggtc ggggtggccg tagccgccgt tggggtcgta ggtcacaacg
    18241 acgtgggggc gctgctcgcg aatgagcgcg acgagggcgc ccacggtctc acgttcgtcg
    18301 gcgtcgatga accgctgtcg aggccgcttc ggggtgccgc gcatgcccga atcacgccat
    18361 cggccggcgc cgccgagata gatcggtgcg ctgacaccca gggcccgcag tgcggtggtc
    18421 agctcgccga tgcgatagcc gccgagttgg tccgcgcgat cgacagtgag ctccgcccaa
    18481 cggtcgccga tgacctcgcc ctcctcaccg ag
//
LOCUS       DASSGQ010000343        12420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_419756,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000343 DASSGQ010000000
VERSION     DASSGQ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12420
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419756"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VFI55_15140"
     CDS             complement(<1..663)
                     /locus_tag="VFI55_15140"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HET7075396.1"
                     /translation="MSPRPRSRKRPRPRRPPPTSLTRPPRISEDVRLRLHIAYDGTDF
                     AGWAAQAGQRTVAGVLDEALTTVFRAPVHLRAAGRTDAGVHATGQVTHVDVPVDAVPN
                     AYPRSPRAGEPEFLPLLRRLGRFLPTDVRVLDIVRAPAGFDARFSALRRHYVYRLSTA
                     PYGVEPHRARYVTAWPRGLDLDAMVAASRDLLGLHDFAAFCRHRDGATTIRDLQRLDW
                     SRD"
     gene            complement(581..1207)
                     /gene="rplQ"
                     /locus_tag="VFI55_15145"
     CDS             complement(581..1207)
                     /gene="rplQ"
                     /locus_tag="VFI55_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET7075397.1"
                     /translation="MPKPTKGPRLGGSSSHQKALLANLATSLFEHGRIKTTEPKARAL
                     RPYAEKLITHAKKGTLHNRREVLKKIRDKDVVHALFAEIGPFFADRNGGYTRIIKVEA
                     RKGDNAPMAVIELVREKTVTSEADRARRVKASKKAAPPAAAAPQTVVEPEAVVEPEAD
                     EASAATEPDEAAAETQADEAAAESPAEEPEAAKAEAAPTDKPDEAAEN"
     gene            complement(1249..2292)
                     /locus_tag="VFI55_15150"
     CDS             complement(1249..2292)
                     /locus_tag="VFI55_15150"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET7075398.1"
                     /translation="MLISQRPTLSEEILTDNRSQFVIEPLEPGFGYTLGNSLRRTLLS
                     SIPGAAVTSIRIDGVLHEFTTVPGVKEDVTDIILNLKGLVVSSEEDEPVTMYLRKQGP
                     GEVTAGDIVPPAGVTVHNPGMHIATLNDKGKLEVELVVERGRGYVPAVQNRASGAEIG
                     RIPVDSIYSPVLKVTYKVDATRVEQRTDFDKLILDVETKSSITPRDALASAGKTLVEL
                     FGLARELNVEAEGIEIGPSPAEADHIASFALPIDDLDLTVRSYNCLKREGVHTVGELV
                     SRTESDLLDIRNFGQKSIDEVKVKLHQLGLSLKDSPPTFDPSEVAGYDVATGTWSTEG
                     AYDDADYAETEQL"
     gene            complement(2378..2983)
                     /gene="rpsD"
                     /locus_tag="VFI55_15155"
     CDS             complement(2378..2983)
                     /gene="rpsD"
                     /locus_tag="VFI55_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET7075399.1"
                     /translation="MARYTGPITRKSRRLRTDLVGGDQAFEKRPYPPGQHGRARIKES
                     EYLLQLQEKQKARFTYGVMEKQFRRYYEEAVRHTGKTGENLLQILESRLDNVVYRAGL
                     ARTRRMARQLVSHGHFTVNGVRVDVPSYRVSQYDIIDVRDTSLNTVPFQIARETAGDR
                     PIPGWIQLVGERQRILIHQLPDRAQIDVPLTEQLIVEYYSK"
     gene            complement(3008..3424)
                     /gene="rpsK"
                     /locus_tag="VFI55_15160"
     CDS             complement(3008..3424)
                     /gene="rpsK"
                     /locus_tag="VFI55_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011207365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET7075400.1"
                     /translation="MPPKKAAASAPKKGQKTRRREKKNVPHGAAHIKSTFNNTIVSIT
                     DPQGNVIAWASSGHVGFKGSRKSTPFAAQLAAENAARKAQEHGVKKVDVFVKGPGSGR
                     ETAIRSLQAAGLEVGAISDVTPQPHNGCRPPKRRRV"
     gene            complement(3428..3802)
                     /gene="rpsM"
                     /locus_tag="VFI55_15165"
     CDS             complement(3428..3802)
                     /gene="rpsM"
                     /locus_tag="VFI55_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET7075401.1"
                     /translation="MARLVGVDLPRDKRMEIALTYIFGVGRTRSNEILAATGIDKDLR
                     SKDLTDDQLTHLRDYIEANLKVEGDLRREVQADIRRKIEIGCYQGLRHRRGLPVRGQR
                     TKTNARTRKGPKRTIAGKKKAR"
     gene            complement(4048..4161)
                     /gene="rpmJ"
                     /locus_tag="VFI55_15170"
     CDS             complement(4048..4161)
                     /gene="rpmJ"
                     /locus_tag="VFI55_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET7075402.1"
                     /translation="MKVNPSVKPICDKCRVIRRHGRVMVICSDPRHKQRQG"
     gene            complement(4212..4433)
                     /gene="infA"
                     /locus_tag="VFI55_15175"
     CDS             complement(4212..4433)
                     /gene="infA"
                     /locus_tag="VFI55_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET7075403.1"
                     /translation="MAKKDGAIEVEGRVVEPLPNAMFRIELENGHKVLAHISGKMRQH
                     YIRILPEDRVVVELSPYDLSRGRIVYRYK"
     gene            4687..6366
                     /locus_tag="VFI55_15180"
     CDS             4687..6366
                     /locus_tag="VFI55_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7075404.1"
                     /translation="MTRPLTGPGSQPRKPVLLTVDDDPAVSRAVARDLRRHYGEDHRI
                     VRAESGADALETLKQLKLRGETVATLIADYRMPQMTGIDFLEQAMDLYPSARRVLLTA
                     YADSHAAIDAINVVDLDHYLLKPWDPPEEKLYPVIDALLDAWKRAPEHPIPHTKVVGH
                     RWSARSWQVRDFLARNGLYYNWFMADEPEGERLLQAAGEDGLRLPVVITERGTALVEP
                     SDAELADTLGLTTTPSQEFYDLVVIGGGPAGLAAAVYGASEGLRTVLIERTATGGQAG
                     QSSRIENYLGFPDGVSGGQLAQRARKQAEKFGAELITARAATALEINGPKRTIRFADG
                     GSIDTHAVIVATGVSYRQLTATGCEELVGRGVYYGAASSIASECDGEEVYVIGGANSA
                     GQAAMHLSREAKSVTILVRAPSLEASMSYYLIQQIEQNPKITVRTCTEVQCATGTDHL
                     EKLTLVDRRTGDTEEVGCGRMFIFIGAAPRTEWLEGVLARDDHGFILTGPDLRNVCGW
                     TLDRPPHHLETSVPGVFVAGDVRADSAKRVAAAVGEGSMAVMLVHRYLAES"
     gene            6403..7917
                     /locus_tag="VFI55_15185"
     CDS             6403..7917
                     /locus_tag="VFI55_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7075405.1"
                     /translation="MESVTAKTPCEPDELRTLFLFEALNDDQLAVLCANGHIKDYQPG
                     PICLEGQPATCFYVLIEGELMMSKLSGGRDIETNRTSQRGVYCGAWRAFTGKKQQNYD
                     ASVRVTKPSRFFVMDAPVFAQFMKDQFPMAVHLLDGIAVGTDRTRRIIDNREKLLALG
                     RLSAGLTHQLNNPAAAIARAGADLRGRIAGMRHKVAMLADGTITAETLNALVRLQEAV
                     AEQVAKSASQHLGAMETADREDAIGEWLEDHGIDGGWDIAPTFVEGGIDTDWLERISA
                     TADELGSASLEQAIRWINYTIESELLMNQIAEASKRISALVSDAKQYSQLDRAPFQVA
                     DVHALLHSTLAMFADRLNKDGSKEGGKDAKTITVVKDFDKSLPEIPCYPGDLNQVWTN
                     IIDNALAAMRDEGGTLTIRTCREGEETIRVEICDTGPGIPDDVRNHIFEPFFTTKPFG
                     EGTGLGLDLAFNIVVKNHRGDLRVESRPGDTRFIVLLPLEAPPATASLTESEPE"
     gene            7946..8800
                     /locus_tag="VFI55_15190"
     CDS             7946..8800
                     /locus_tag="VFI55_15190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET7075406.1"
                     /translation="MTDSHASSASKPKLGRFGSFGRGVTPQQAKEIESLGYGAVWVGG
                     SPPAELAWVEPLLEATTTLQVATGIVNIWSAPAAPVAESFHRIDKAYPGRFLLGIGVG
                     HPEAVTEYRKPYDALVEYLDKLDEYGVPADRRVVAALGPRVLKLSAERSAGAHPYLTT
                     PEHTAQARELIGPSALLAPEHKVVLTTDAEEARSVGRKALDIYFNLANYRNSWKRLGF
                     SDDEVTRPGSDRLVDAVVAYGTPEQIAARLKQHLEAGADHVAVQVLTKDENLVSALAE
                     LAGPLGLS"
     gene            8832..9698
                     /locus_tag="VFI55_15195"
     CDS             8832..9698
                     /locus_tag="VFI55_15195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET7075407.1"
                     /translation="MSEAVSLKPDLGRFGVFLATRSITPELAAAIESLGYGAVWIGGS
                     PDAELAWVDPALTQTTSLQLATGIVNIWSAPAPAVADSFARIEGAHPGRFLLGIGAGH
                     REHTEEYVKPYDALVGYLDELDAAMVPTSRRVLAALGPRVLRLAAERSAGAHPYLTTP
                     EHTAKAREMLGNSVFLAPEHKVVLTTDTGKARAIGRETVDHYLGLSNYVNNWLRLGFT
                     QDDVRRPGSDRLIDAVVAYGTPDAVAQRLNEHVEAGADHVAIQVLGGWDEATLLPALS
                     ELAGPLGLTPPS"
     gene            9725..10720
                     /gene="rfbB"
                     /locus_tag="VFI55_15200"
     CDS             9725..10720
                     /gene="rfbB"
                     /locus_tag="VFI55_15200"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET7075408.1"
                     /translation="MRLLVTGGAGFIGANFVHSTVREHPEDDVTVLDALTYAGRRESL
                     ADVEDAIRLVVGDIADAELVSRLVAESDAVVHFAAESHVDNALDDPEPFLHTNVVGTF
                     AILEAVRRHGVRLHHISTDEVYGDLELDDPQRFTESTPYNPSSPYSATKAGADMLVRA
                     WVRSYGVRATISNCSNNYGPYQHIEKFIPRQITNVLTGRRPKLYGTGANVRDWIHVDD
                     HNSAVRRILDKGRIGQTYLISAEGERDNLTVLRTLLRMMGRDPDDFDHVTDRVGHDLR
                     YAIDPSLLYDELCWAPKHTDFEEGLQTTIDWYRANESWWRPLKDAAEVRYEERGQ"
     gene            10722..11339
                     /gene="rfbC"
                     /locus_tag="VFI55_15205"
     CDS             10722..11339
                     /gene="rfbC"
                     /locus_tag="VFI55_15205"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HET7075409.1"
                     /translation="MKVRELNIPGAFEITPTLHGDSRGLFFEWLTDRGFSSFAGHRLD
                     VRQANCSVSAAGVLRGLHFAQLPPSQAKYVTCVSGAVFDVVVDIRLGSPTFGQWDSVL
                     LDDKDRRTIYISEGLGHGFVALQDNSTVMYLCSAEYDPQREHTICATDPALAIDWPLV
                     DGAAPSLSDRDAVAPTFEEVRASGLLPTWAEAQRFADDLRAASNQ"
     gene            11336..11824
                     /locus_tag="VFI55_15210"
     CDS             11336..11824
                     /locus_tag="VFI55_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LpqH"
                     /protein_id="HET7075410.1"
                     /translation="MKPELLIGLTAATIVAGIAGCSNGNTASGPSSATSSSATTNSSH
                     VSTSSPAAAGPAQVTIGGQPQNVSGLVVCSTNDGKFSIAVGDMATGIIVGLEPDASVV
                     HNAGLGTVDGVVMSFTEGVPGENASATKTGNAYQITGTASGVDNAGRQVTKPFEVDVT
                     CP"
     gene            complement(11831..12202)
                     /locus_tag="VFI55_15215"
     CDS             complement(11831..12202)
                     /locus_tag="VFI55_15215"
                     /inference="COORDINATES: protein motif:HMM:NF026182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HET7075411.1"
                     /translation="MPLYRITTPEGLLSPEAKAALASEITDFHSGMAGLDVAYTKLVF
                     DSFAPGDGFVGGTAAPAVLLTVKIREGRPADYKNKLLFGLKSLLQGATGAQDVDMLLA
                     LEEMPASNAIELGELMPDIGK"
     gene            complement(12210..>12420)
                     /locus_tag="VFI55_15220"
     CDS             complement(12210..>12420)
                     /locus_tag="VFI55_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075412.1"
                     /translation="PISDKRMVGATGGANARFRNHRAREHISEIPRGWVALPSNSRQP
                     TETPPKFEVASQPRQNNAITLSTKG"
BASE COUNT         2097 a         4142 c         4033 g         2148 t
ORIGIN      
        1 gtcgcgcgac cagtccagcc gctgcaagtc gcggatggtg gtggcgccgt cgcgatgacg
       61 gcagaatgcc gcaaagtcgt gcaatcccaa caggtctcgc gacgcggcga ccatcgcatc
      121 gaggtccagg ccgcgcggcc aggcggtcac gtaccgcgcc cgatgcggct ccacaccgta
      181 gggcgccgtc gacagccggt acacataatg acgtcgcagc gcggagaatc gcgcgtcgaa
      241 acccgctggg gcgcggacga tatcgagcac gcggacgtcg gtgggcagaa accggccgag
      301 acggcgcagc aacggcagga attccggttc accggcgcgt ggcgagcgcg gataggcgtt
      361 tggcacggcg tcgacgggca catcgacgtg ggtcacctgc ccggtggcat gaaccccggc
      421 gtcggtgcgc ccggccgcac gcaggtgcac cggcgcgcgg aagaccgtcg tcagcgcttc
      481 gtctagaacg ccggccacgg tacgctgccc ggcctgtgct gcccagcccg cgaaatcggt
      541 tccgtcgtag gcgatgtgca gccgcagacg gacgtcctca ctaattctcg gcggcctcgt
      601 caggcttgtc ggtgggggcc gcctcggcct tggccgcttc cggctcctcg gccggggact
      661 cagccgcggc ctcgtcagcc tgcgtctcag ccgcggcctc gtccggctcg gtggctgccg
      721 aggcttcgtc ggcctccggc tcaaccacgg cctccggctc aaccaccgtc tgcggtgccg
      781 ccgccgcggg cggggccgcc ttcttcgacg ccttcacccg gcgcgcccga tcggcctccg
      841 acgtcaccgt cttctcgcgc accagctcga tcacggccat cggagcgttg tcgcccttgc
      901 gcgcttcgac tttgatgatg cgggtgtagc cgccgtttcg atcggcgaag aacggcccga
      961 tctccgcgaa cagggcgtgc accacgtcct tgtcgcggat cttcttgaga acctctcgac
     1021 ggttgtgcag cgtgcccttc ttcgcgtgcg tgatcagctt ctccgcatag ggccgcagcg
     1081 cacgcgcctt cggctcggtc gtcttgatcc gcccgtgctc gaacagcgac gtggccaggt
     1141 tggccagaag ggccttctgg tgcgacgacg acccgccgag gcgagggccc ttggtgggct
     1201 tgggcattgc tgacgctcct gtctagatgt cggctgagtc cagctccgct agagctgttc
     1261 ggtttcggcg tagtccgcgt cgtcgtaagc gccctcggtg gaccaggtgc cggtggccac
     1321 gtcgtagccc gcgacctccg acgggtcgaa ggtcgggggg ctgtccttga gcgagagacc
     1381 cagctggtgc agcttgacct tcacctcgtc gatggacttc tgcccgaagt tgcggatgtc
     1441 gagcaggtcg gactcagtgc gcgagaccag ctctccgacg gtgtgcacgc cctcgcgctt
     1501 gaggcagttg taggaccgca cggtcagatc caggtcgtcg atcggcagcg cgaacgacgc
     1561 gatgtggtcg gcctccgccg gcgacggccc gatctcgatg ccctcggcct cgacgttgag
     1621 ttcccttgcc agaccgaaca attcgaccag cgtcttaccc gccgacgcga gtgcgtcgcg
     1681 cggggtgatg gagctcttgg tctcgacgtc caggatcagc ttgtcgaagt cggtgcgctg
     1741 ctcgacacgg gtggcgtcca ccttgtaggt caccttgagc accggcgagt agatggaatc
     1801 gactgggata cggccgattt cggccccaga ggcccggttc tgcaccgccg ggacgtagcc
     1861 gcggccgcgc tccacgacga gctcgacctc gagcttgccc ttgtcgttca acgtcgcgat
     1921 gtgcatgccg gggttgtgca cggtcacacc ggcgggcggc acgatgtcac ccgcggtgac
     1981 ctcacccgga ccttgcttgc gcaggtacat ggtgaccggc tcgtcctcct ccgaggacac
     2041 gaccagcccc ttgaggttca ggatgatgtc ggtgacatct tccttcacgc caggcacggt
     2101 ggtgaactcg tgcagcacgc cgtcgatgcg aatgctggtg acggccgcgc cggggatcga
     2161 ggacagcaat gtacgacgca gcgaattgcc aagggtgtaa ccgaatcccg gctccaacgg
     2221 ctcgatgacg aactgggagc ggttgtcggt gaggatctcc tcggacaggg tgggccgctg
     2281 tgagatcagc atggtttttc ttcttctcct tctcggcacc cgctatttga tgccgttggg
     2341 tgtcccgcca ctgcggccgc agtcgcggaa gtctgcgcta cttcgagtag tactcgacga
     2401 tcagctgctc ggtcagtggc acgtcgatct gcgcccggtc cggcaactgg tggatcagga
     2461 tgcgttggcg ctcccccacg agctgaatcc agcccgggat cgggcggtcg cccgccgtct
     2521 ctcgcgcgat ctggaagggc accgtgttca gcgacgtgtc ccgcacatcg atgatgtcgt
     2581 actgcgacac ccgatagctg ggcacgtcga cgcgcacgcc gttgacggtg aagtgtccgt
     2641 ggctgacgag ctgacgagcc atccggcggg tacgcgccag accggcgcgg tacacgacgt
     2701 tgtccagccg gctctccagg atctgcagca ggttctcacc tgtcttgccg gtgtggcgaa
     2761 cggcctcttc gtagtagcgg cggaactgct tttccatcac gccgtaggtg aagcgagcct
     2821 tctgcttctc ctgcagctgc agcagatatt cgctttcctt gatccgcgcg cgaccgtgct
     2881 ggccgggcgg atagggacgc ttctcgaagg cctggtcgcc gccgacgagg tcggtgcgca
     2941 gccggcgcga tttgcgtgtg atgggtccgg tgtaacgagc cattagatat cttcctccta
     3001 gaccctgcta gaccctgcgt cgcttgggcg gacggcagcc gttgtgcggc tgaggggtga
     3061 cgtcagaaat cgcgccgacc tccaggcccg cggcctgcag cgaccggatc gcggtctccc
     3121 gacccgagcc cgggcccttc acgaacacgt cgaccttctt gacgccgtgc tcctgggcct
     3181 tgcgtgcggc gttctcggcg gccagctggg cggcgaacgg cgtggatttc cgcgagccct
     3241 tgaagcccac gtgacccgac gacgcccagg cgatgacgtt gccctgcggg tcggtgatcg
     3301 acacgatcgt gttgttgaac gtgctcttga tgtgcgcggc gccgtgcggg acgttcttct
     3361 tttcccgcct gcgggtcttc tgccccttct tgggcgctga ggcggctgcc ttcttcggtg
     3421 gcatcggtta cctggccttc ttcttgcctg cgatggtgcg cttggggccc ttgcgggtgc
     3481 gcgcgttggt cttggtccgc tggccgcgca ccggcaggcc gcgacggtgt cgcagaccct
     3541 ggtagcagcc gatctcgatc ttgcgacgga tgtcggcctg cacctcccgg cgcaggtcgc
     3601 cctccacctt cagattggct tcgatgtagt cacgcaggtg ggtcagttgg tcgtcggtga
     3661 ggtccttgct gcgcaggtcc ttgtcgatgc cggtggccgc cagaatctcg ttcgagcggg
     3721 tacggccgac gccaaagatg taggtcaggg cgatctccat ccgcttgtcg cgcgggagat
     3781 cgacgccgac tagtcgagcc ataggtggcg tttcctcttt ttctaggcgg aggtttggtc
     3841 ccagtccgtt cccgattgtt cggggcccgg cctccgtccg ggcgtgcatg agctggccca
     3901 tctcattaga tgccagccgc tcattggtgc tgggaggtct gcattcagtt gtcgtacgcc
     3961 ctgaagaggg ctagcggagc gcggcccgat tgttcggctt ccccagaggg aagaacccca
     4021 cctcagaccg cgggtatcgg cgccgaacta tccctggcgc tgcttgtggc gcggatctga
     4081 gcagatcacc atgacccgcc catgccgacg gatcaccctg cacttgtcgc agattggctt
     4141 gacgctcggg ttcaccttca cggctcgttc gatcctgttc tgtctaatcg tcggttattg
     4201 atgcgggctt tttacttgta gcggtacacg atgcggccac gggacaggtc gtagggagac
     4261 aactccacca ccacccggtc ctcgggcagg atgcggatgt agtgctgccg catcttgccg
     4321 ctgatgtggg caagcacctt gtggccgttc tccagctcaa tgcgaaacat cgcattgggc
     4381 aggggctcga ccacgcggcc ctcgacctct atggcgccgt ccttcttggc cataacttcc
     4441 cagaaatcct cgtcgttagt ttcgtatcga tttgcgccct gaccggcgca acatttaccc
     4501 cgacttcaaa cgtgagccgg tgacaggaaa ttcctgggaa ctgggcacgc aaaaaagccg
     4561 gcgcgagcgc cgcaccgtta ctccacgata cccggtcggt cgacgcggcc aaaatcgctc
     4621 gtgaggacgg ccgaagccgc tgcctcgctt cccgcatgtc ggcgggctag agcatactta
     4681 tttccgatga caagacccct tacaggcccc gggtcacagc cacgcaaacc cgtgttgctg
     4741 accgtcgacg atgatcccgc ggtatcgcgc gcagtggccc gcgacctgcg gcgccattac
     4801 ggcgaggacc accgcatcgt gcgggccgaa tccggtgccg acgccctgga aacgctcaag
     4861 caactcaaac tgcggggcga aacggtcgcc acgctcatcg ccgactaccg aatgccccag
     4921 atgacgggca tcgatttcct cgaacaggcg atggacctgt atccctcggc acggcgggta
     4981 ctgctgaccg cctacgccga cagccacgcc gccatcgatg cgatcaacgt cgtcgacctg
     5041 gaccattacc tgctcaaacc gtgggatcca ccggaggaaa agctctatcc cgtcatcgac
     5101 gcgctgctgg acgcctggaa gcgcgccccg gaacacccga ttccgcacac caaggtggtc
     5161 gggcatcgct ggtcggcgcg atcctggcag gttcgcgact tcctggcccg caacggcctc
     5221 tactacaact ggttcatggc cgacgagccg gaaggcgagc gcctgctgca ggcggccggc
     5281 gaggacggcc tgcggctacc ggtcgtcatc accgaacgcg gcaccgccct cgtcgaaccc
     5341 agcgatgccg agctggccga cacgctgggg ctgaccacca caccgtcgca ggagttctac
     5401 gacctcgtcg tcatcggtgg cgggcccgcg ggcctggcgg ctgccgtcta cggtgcctcc
     5461 gagggcctgc gcaccgtgct catcgaacgc accgcgaccg gcggccaggc cggccagagc
     5521 tcgcggatcg agaactacct cggctttccc gacggggtgt cgggcggcca gctggcccag
     5581 cgagcgcgca agcaggcgga gaagttcggt gccgaactca tcaccgcccg cgcggccacc
     5641 gcgctggaga tcaacggacc aaaacgcaca attcggttcg ccgacggcgg ctccatcgac
     5701 acgcatgcgg tcatcgtggc caccggcgtg tcataccgcc aactgaccgc aaccgggtgc
     5761 gaagagctgg tcggccgtgg cgtctactac ggagcggcct catccatcgc ctcggagtgc
     5821 gacggcgaag aggtctatgt catcggcgga gccaattcgg ccggccaggc cgcgatgcac
     5881 ctgtcccgcg aggccaaatc ggtgaccatc ctcgtgcgtg ccccatcgct ggaggcctcg
     5941 atgtcgtact acctgatcca gcagatcgag cagaacccca agatcaccgt ccgcacctgc
     6001 accgaagtcc aatgcgccac cggaacggac cacctggaga aactgaccct ggtcgacaga
     6061 cggaccggcg acaccgagga ggtcggctgc gggcggatgt tcatcttcat cggcgccgca
     6121 ccgcgcacgg agtggctcga aggagtcctc gcccgcgacg accatggctt catcctcacc
     6181 ggcccggatc tgcgcaacgt gtgtggctgg accctggacc gcccaccgca ccacctggaa
     6241 acgagcgtgc cgggagtgtt cgtggccggc gacgtccgcg ccgactccgc caagcgggtc
     6301 gcggccgcgg tcggcgaagg atcgatggcc gtgatgctgg ttcaccgcta cctggctgag
     6361 tcctgatgca ggttctgacg cacgtcctga tggagggttc gagtggagtc cgtaaccgcc
     6421 aagacaccgt gtgagcccga cgagctgcgc accctgtttc tgttcgaggc cctcaacgac
     6481 gaccagctcg cggtgttgtg cgccaacgga cacatcaagg actaccaacc cgggccgatc
     6541 tgcctggaag gccagccggc cacctgcttc tacgtcctca tcgagggcga gctgatgatg
     6601 tcgaagctct ccggcggtcg ggacatcgaa accaaccgga catcgcagcg cggcgtctat
     6661 tgcggggcct ggcgggcgtt caccggtaag aagcagcaga actacgacgc ctcggtgcgc
     6721 gtaaccaagc cgtcgcggtt cttcgtgatg gacgcgcccg tattcgccca gttcatgaag
     6781 gaccagttcc cgatggcggt ccacctgctc gacggcatcg ccgtcggcac cgaccgcacc
     6841 cgccgcatca tcgacaaccg cgagaagctg ctggcgctgg gccggttgtc ggccgggctg
     6901 acccaccagc tgaacaatcc cgccgcggcc atcgcccgag ccggcgccga cctgcgcggc
     6961 cggatcgccg gcatgcgcca caaggtggcg atgctcgccg acggcaccat caccgccgag
     7021 acgctgaacg ccctggtccg gctgcaggag gcggtcgccg agcaagtcgc caagtcggca
     7081 tcgcagcacc tgggcgcgat ggaaactgcc gaccgcgagg acgccatcgg cgaatggctg
     7141 gaggaccacg gcatcgacgg cgggtgggac atcgcaccga cgttcgtcga aggcggcatc
     7201 gacaccgact ggctggagcg catttccgcg accgccgacg agctcgggtc agcatcgttg
     7261 gagcaggcga tccgatggat caactacacc atcgagagcg agctgctgat gaaccagatc
     7321 gccgaagcga gcaaacggat ctcggcgctg gtctccgacg ccaaacagta ttcccagctg
     7381 gaccgggctc ccttccaggt ggccgacgta catgccctgc tacacagcac gctggccatg
     7441 tttgccgacc gtctgaacaa ggatgggagc aaagaaggtg gcaaggacgc caagaccatc
     7501 accgtggtca aggacttcga caagtccctt cccgaaatcc cctgttaccc aggcgatctc
     7561 aatcaagtgt ggaccaacat cattgacaac gccctggccg cgatgcgcga tgagggcggg
     7621 acgctgacca tccgcacctg ccgggaaggc gaggagacga tccgggtcga gatctgcgac
     7681 accgggccgg ggattcccga cgacgtgcgg aaccacatct tcgagccgtt cttcaccacc
     7741 aaaccgttcg gtgaaggtac cggcctcggg ctggacctgg cgttcaacat cgtcgtgaag
     7801 aatcatcgcg gggatctgcg ggtcgagtcg cggcccggcg atacgcgatt catcgtgcta
     7861 ctaccgctgg aggcgccacc ggcgacggca agtcttacgg aatcggaacc ggaatagcgt
     7921 caccgtaggg ccggttggag agaccgtgac ggattctcat gccagttctg caagcaaacc
     7981 caagctaggc cggttcggat cgttcggacg gggcgtgacg ccccagcaag ccaaagaaat
     8041 cgagtccctg ggctacgggg ccgtctgggt gggtggctcc ccgcccgccg agctggcctg
     8101 ggtggaaccc ctgcttgagg cgacgaccac gctgcaggtg gccactggca tcgtcaacat
     8161 ctggtcggcg cccgccgcgc cggtagccga atcgtttcac cgcatcgaca aggcgtaccc
     8221 gggccgcttc ctgctcggca tcggcgtggg ccaccccgag gccgttaccg agtaccgcaa
     8281 gccctacgac gcactggtgg agtacctgga caagctcgac gagtacggcg tgccggccga
     8341 ccgccgcgtg gtggcggccc tgggtccccg ggtgctgaag ctgtcagccg agcgcagcgc
     8401 cggcgcccac ccctacctga ccaccccgga gcacaccgcg caggcccgtg agctgatcgg
     8461 cccgtcggcg ttgctggcgc ccgaacacaa agtggtactg accacggacg ccgaggaagc
     8521 ccgttcggta ggccgcaaag cgctcgacat ctacttcaat ctggccaatt accgcaacag
     8581 ctggaagcgg ttgggctttt ccgacgacga ggtcacccgc ccgggcagcg accgcctggt
     8641 cgacgcggtg gtggcatacg gcaccccgga gcaaatagcg gcgcggctca aacagcacct
     8701 cgaggcgggc gccgaccacg tggctgtgca agtcctcacc aaggacgaaa acctggtctc
     8761 ggcgctggcc gaactggcgg ggccgctcgg actgagctag tgggcgccaa gcatcctcga
     8821 gggggattta gatgagtgag gcggtttcac tgaaacccga tctgggccgg ttcggcgtat
     8881 ttctggccac ccgctccatc acgccggaac tggccgcagc gattgaatcg ctcgggtacg
     8941 gcgccgtctg gatcggcgga tcaccagatg cggagctggc ctgggtcgac ccggccctta
     9001 cgcagaccac gtcgttgcaa ctggccaccg ggatcgtcaa catctggtcc gcgcctgcac
     9061 cggcggtcgc cgactccttt gcacgcatcg aaggcgccca ccccgggagg tttttgctcg
     9121 gcatcggggc gggccaccgc gagcacaccg aagaatacgt gaaaccgtac gacgcgctcg
     9181 tcggttacct cgacgaactc gacgccgcga tggtgccgac cagccgccgg gtgctcgcgg
     9241 cgctcgggcc gcgggtgttg cggctggcgg ccgaacgcag cgccggcgcc cacccgtatc
     9301 tgaccacgcc ggagcacacg gccaaggcgc gcgagatgct gggcaactcg gtgttcttgg
     9361 cgccagagca caaggtggta ctcaccaccg atacgggcaa ggcccgggcg atcggccgcg
     9421 agaccgtcga ccactatctg gggctgagca actacgtgaa caattggctg cggctggggt
     9481 tcacccaaga cgatgtgcgc aggccgggca gcgacaggct gatcgacgcg gtggtggcct
     9541 acggaacccc ggacgccgtc gcgcagcgcc tcaacgagca cgtggaggcc ggcgccgacc
     9601 acgtcgcgat ccaggtgctc ggcggctggg atgaagcgac gctgctgccg gcgctgagtg
     9661 aactggccgg gccgttaggg ctgactcccc caagctgagg actcccctcg gttagggtgt
     9721 ggccatgcga ttgctggtca ccgggggagc cggcttcatc ggcgccaatt tcgtgcacag
     9781 cacggtacgg gagcatcccg aagacgacgt gacggtgctg gacgccctta cctatgccgg
     9841 gcggcgcgaa tcgctggccg acgtcgaaga cgccatcagg ctggtggtgg gcgacatcgc
     9901 cgacgccgag ctggtgtccc ggctggtcgc cgagtccgac gcggtcgtgc atttcgccgc
     9961 tgagagtcac gtggacaacg cgctggacga cccggagccg tttctgcaca ccaacgtggt
    10021 cgggacgttc gccatcctgg aggcggtgcg acgccacggc gtgcggctgc accacatctc
    10081 caccgacgag gtctacggag acttggagct cgatgatccg cagcggttca ccgagtcgac
    10141 tccctacaac ccgtcgagtc cgtattcggc gacgaaggcc ggcgccgaca tgctggtccg
    10201 ggcctgggtg cggtcctacg gggtgcgcgc gacgatctcc aactgctcca acaactatgg
    10261 gccctaccag cacatcgaga aattcattcc ccgtcagatc accaacgtgc tcaccggcag
    10321 gcggcccaag ttgtacggca cgggtgccaa cgtgcgcgac tggatccacg tggatgacca
    10381 caacagcgcg gtgcggcgaa tcctcgacaa gggccggatc ggccagacct acctgatcag
    10441 cgccgagggc gagcgcgaca atctgaccgt gctgcgcacg ctgctgcgga tgatgggtcg
    10501 ggaccccgac gacttcgacc atgtcaccga ccgggtcggc cacgacctgc ggtacgccat
    10561 cgacccctcg ttgctgtacg acgaattgtg ctgggcgcca aagcacaccg acttcgagga
    10621 aggtttgcag accaccatcg actggtatcg tgccaacgag tcgtggtggc gtccgttgaa
    10681 ggacgccgcg gaagtccgct acgaggaacg tggtcagtga gttgaaagtt cgcgaactca
    10741 acatccccgg cgcctttgag atcaccccca ccctccacgg tgattcccgt ggcctgtttt
    10801 tcgaatggct caccgatcgc gggttcagct cgttcgccgg ccatcggttg gacgtgcggc
    10861 aagccaactg ttcggtctcg gcggccggtg tgctgcgcgg cctacacttc gcccagctgc
    10921 cacccagcca ggccaagtac gtgacctgcg tgtccggcgc ggtgttcgac gttgtcgttg
    10981 acatccggct gggctcaccg actttcggcc aatgggactc ggttctgctc gacgacaaag
    11041 accggcggac gatctacatc tccgaaggtc tcgggcacgg tttcgttgcg ctgcaagata
    11101 attcgacggt gatgtacctg tgttcggcgg aatacgaccc gcaacgggag cacaccatct
    11161 gcgcgacgga tccggcgctg gccatcgact ggccgctggt cgacggcgcc gccccgagcc
    11221 tgtccgaccg cgacgccgtg gcgccgacct tcgaggaagt acgcgcttcc ggcctcctgc
    11281 ccacctgggc ggaggcacag cgatttgccg acgatttgcg ggcagcgagc aatcagtgaa
    11341 acctgagttg ctgatcggcc ttaccgcggc aaccatcgtg gcgggcatcg ccggctgttc
    11401 gaacggcaac accgcatccg gcccgtcgag cgcgacgtca tccagcgcaa ccaccaactc
    11461 gtcgcacgtc tcgaccagca gccccgctgc ggccgggccg gcacaggtca ccatcggcgg
    11521 ccaaccccag aacgtcagcg ggctggtggt gtgctcgacc aacgacggca agttctccat
    11581 cgcggtcggc gacatggcca ccggcatcat cgtcggtctc gaacccgatg cgtcggtggt
    11641 ccacaacgca gggctcggca ccgtcgacgg cgtggtgatg agcttcaccg aaggtgtgcc
    11701 cggcgagaac gccagtgcca ccaagaccgg caacgcctac cagatcaccg gcacggccag
    11761 cggcgtcgac aacgcgggcc ggcaggtcac caagcctttc gaagtcgacg tcacctgccc
    11821 ctagctccgc ctacttgccg atatcgggca tcagctcgcc cagttcgatg gcgttgctgg
    11881 ccggcatctc ctcgagcgcc agcagcatgt cgacatcctg cgcgccggtc gcgccctgaa
    11941 gtagcgactt gagcccaaac agcagcttgt tcttatagtc ggccgggcgt ccctcccgga
    12001 tcttcaccgt caagagcacc gctggcgcgg ctgtgccacc gacaaacccg tcaccgggtg
    12061 caaagctgtc gaagacgagc ttcgtgtacg ccacgtcgag tccggccatt ccgctgtgga
    12121 agtcggtgat ctccgaggcg agcgccgcct tcgcttcggg ggaaagcaat ccttccggcg
    12181 tggtgatccg atagaggggc atggtgacat cagcctttcg tcgagagcgt gatcgcgtta
    12241 ttttgtcgcg gctgcgaggc aacttcgaac ttgggtgggg tttcggtggg ttgtcgggag
    12301 ttcgaaggca acgccaccca cccccgcggg atctccgaaa tatgctctcg ggcacggtga
    12361 ttgcgaaaac gtgcatttgc gccgcccgta gcgcctacca tccgcttgtc gctgatcggg
//
LOCUS       DASSGQ010000344         6887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1057990,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000344 DASSGQ010000000
VERSION     DASSGQ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057990"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VFI55_15225"
     CDS             complement(<1..351)
                     /locus_tag="VFI55_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7075413.1"
                     /translation="MNLELTDEQVALRDTTRRYLVEKASISGHVRALLDDPTGSTGAV
                     WRGLADLGTTGLLVPPQYGGSGMTMVEAGIVAEELGAALHPGPWSSSAVAAPRALARL
                     GAGDAAASILSGIAD"
     gene            complement(348..1526)
                     /locus_tag="VFI55_15230"
     CDS             complement(348..1526)
                     /locus_tag="VFI55_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7075414.1"
                     /translation="MDFSYPAEVEQFRAELRDWLSGNLTPELVAARRPSGRDDATFEL
                     LRAWNRTMADAGWAAVSWPREYGGRGATVLEQLVYTEETTRARAPLPLNVIGMNNIAP
                     AIMQYGTESQKLALLPRMMRADDIWCQGMSEPEAGSDLAALRTRAVRDSPYGGDFVVN
                     GQKIWTSLGHRANWCQLYVRTDPQAPKHKGISCLIVDMALPGIEVRPLVTLNGDTDFA
                     EVFFRDVRVPAEALLGPLNGGWQVATTTLSHERAGAARLYAEMQARLEELVADLTAAD
                     AGAAHGVLQDPVTLRRLGEIAVRIKYLEVLCQRSISATLHGESDNAALGSASLAKTVW
                     GEIGQDMAALAFDVLGAQGVAGRWADYRLTSRSLTIAGGTTQINKNVTAQRVLGLPRQ
                     "
     gene            1678..3354
                     /locus_tag="VFI55_15235"
     CDS             1678..3354
                     /locus_tag="VFI55_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7075415.1"
                     /translation="MLASALSGRTLQVAAVRPGEPSWTDGATIFVDAGAQRQLETLAV
                     QASLLAAGSLEPKLLRVLKRRPGATRRYLAVEGRRALAQNADVLPPAAGTLVDSGLAV
                     TADSPAASLHMALSRQAIPDPPDWFGTIHARNLLAANNIRSKAADGAGARRRHAQTPA
                     QFDDHDAAAASQDADIFTSPVGGRGALGRLLGSMLRAVRRLGEGGHPGADAPTHRTFS
                     GIRAGTAALSTAPVGPFDDLAADGDGITYPEWDIHRRCYRPNWCTVREVEPVVRDVGS
                     PDVPHGYGLRRPLARLGLGPDWFHRQVYGDDIDIDAAVEARVAAAAGSLPEEAVYIAP
                     LRRRRDLAVLLLLDVSGSGNEPGAHGGTVHEQQRAAAAALAVSLHALGDRLALYAFHS
                     QGRSSVHVMPVKRFDDDMNASVLRRLYGLTPGAYSRLGAAIRHGAAVLESRGGTSRRL
                     LVVLSDGLAYDHGYERVYGAADARRALAEARGRGVGCVCLTIGAGTDPDELQRVFGSA
                     AHATIARPEELSRVVGPLFRTALRAAEVLRPAARSSLSQISVPRYAARST"
     gene            3369..4181
                     /locus_tag="VFI55_15240"
     CDS             3369..4181
                     /locus_tag="VFI55_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HET7075416.1"
                     /translation="MAVEGVVPRAQPFRPFYVTVGDEEKVFKAAFRQGLSVLLKGPTG
                     CGKTRFLEAMAYDLGRPLITVACHDDLTTADLVGRYLLRGGETEWVDGPLTRAVREGA
                     ICYLDEVVEARQDTTVVLHPLADHRRQLPLERLGVTLDAAPGFCLVVSYNPGYQSVLK
                     DLKDSTRQRMVAIEFGFPAADVEEKIVAHEAGINAETAAELVRLAQAIRRLENGGLRE
                     VASTRVLIAAGRLIAEGLTRSQAARAAIAGPLTDDPHAAAGLSEMIDAYLLD"
     gene            4326..5438
                     /locus_tag="VFI55_15245"
     CDS             4326..5438
                     /locus_tag="VFI55_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7075417.1"
                     /translation="MSYESTAEPIRIGYLFDFLLPQGYPQEMRDDLTRPFDLVFNDGV
                     RERVIDRPVRIVYREVEGLPKGTVKAVIDAYGELVDEGCLVVFGPHITENAVPLREAI
                     EERFRVPAINVCGSDDWLGEWTFAFPQGSMTDEPIFWTDLLAKGGHTQVGVLVEQSLV
                     GESYLKNFRNACRRKGIRIVAEAQVAQTAQDVGTAIRSLHEAKPSALVHCGFGFGVVF
                     INPALAELDWDPPRFTSTAFQNAWINPVMWQAFMGWTGIDQYDEGNGVGQAFLDRYAS
                     EYGRRPQYCVPVVNHDVANALLHAFADAHPLSPRGVKEALERVKMLPAAAGAPGTRVS
                     FGKWTRRAWMGAGYLVARQLDVDGVSSHLVDRFGEE"
     gene            5442..6593
                     /locus_tag="VFI55_15250"
     CDS             5442..6593
                     /locus_tag="VFI55_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spirocyclase AveC family protein"
                     /protein_id="HET7075418.1"
                     /translation="MTTKQTTPSTPSTPSTPSTKESPTRPKKRSVWAIVCILLAAAAL
                     AVVAHYARTGAVSDRIRNPNVVGAPRPVEPLFGYQHWLGLFQIFTIISMAIVIAVYVV
                     VWRRHPAHPILLMGIVTTLIVWQDPIMNWAPYAVYNPQLWHWPEDWPLVSLSPTVEPF
                     LVIGYIMFYLGPYFPAIWILRKIQARRPVDSFVWRHPLITLAAIILPVGIVMDAMLET
                     TLVRTGFYIYDQVIPFGSVFTGKTYQFPFIWETVMVTFVMIPAGVLLYRDDTGRTVAE
                     KLAQRARIFPGRPTLAMFAVMFVLINLAYFAYGTGFAIIKWTKTATSVACPWPYPEAK
                     VYDPQGFYERNGQPGPYSVGIWSTWMSAQPNGRPNVELPADGGRCAPRT"
     gene            6590..>6887
                     /locus_tag="VFI55_15255"
     CDS             6590..>6887
                     /locus_tag="VFI55_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7075419.1"
                     /translation="MSEPRTIVITGASRGLGLASAAHMYQRGWRVVAAMRSPESGLSA
                     LRAKTGAAEDDPRLVAVPLDLTDPASIAAAAKAIEAAAGAPHALVHNAGISAAGA"
BASE COUNT         1086 a         2305 c         2341 g         1155 t
ORIGIN      
        1 gtcggcgatc ccggacaaga tcgaagcggc cgcgtctccg gcaccgagcc tggccagagc
       61 gcgcggcgcg gcgaccgcac tcgacgacca cggccccggg tgcagggcgg cgccgagctc
      121 ttcggccacg atgccggcct cgaccatcgt catgccggaa ccgccgtatt gcggcggcac
      181 caacagcccg gtggtcccga ggtcggcgag gccacgccag acggcgccgg tgctgcccgt
      241 cggatcgtcg agcagcgcac gcacgtggcc cgaaatcgat gctttctcaa caagatagcg
      301 tcgcgtggtg tcgcgcagcg ccacctgctc gtcggtcagt tcgaggttca ttggcgcggc
      361 aatcccagca cgcgttgcgc ggtgacgttc ttgttgattt gggtggtgcc gccggcgatg
      421 gtcagcgagc gcgacgtcag gcggtagtcc gcccatcggc ccgctacccc ctgggctccg
      481 agcacatcga aggccagcgc cgccatgtcc tggccgattt cgccccacac ggtttttgcc
      541 agactggccg acccgagggc ggcattgtcc gactcgccgt gcagagtcgc cgagatcgac
      601 cgctgacaga gcacttcgag atatttgatg cgcaccgcga tctcgccgag gcgccgtagt
      661 gtgaccgggt cttgaaggac gccgtgcgcg gcgccggcat cggccgcggt gaggtcggcc
      721 acgagttcct cgagccgtgc ctgcatctcc gcgtacagcc gcgcggcccc ggcgcgctcg
      781 tggctcagcg tggtggtggc cacctgccac ccgccgttga gcggaccgag caacgcctcg
      841 gccggcaccc gcacgtcacg gaagaacact tcggcgaagt cggtgtcacc gttgagcgtg
      901 acgagcgggc gaacctcgat gcccggcagc gccatgtcga caatcaggca ggagatccct
      961 ttgtgcttcg gagcttgggg atcggtgcgt acgtacagct ggcaccaatt cgcccgatgc
     1021 ccgagcgagg tccagatctt ctgcccgttg accacgaagt cgccgccgta agggctgtca
     1081 cggaccgcgc gggtgcgcag cgcggcgagg tcggatcccg cttcgggttc ggacattccc
     1141 tgacaccaga tgtcgtcggc gcgcatcatc cgggggagca gcgccagctt ctgggattcg
     1201 gtgccgtact gcatgatcgc gggtgcgatg ttgttcatgc cgatgacgtt gagcggcaac
     1261 ggcgctcgcg ctcgggtggt ttcctcggtg tagaccagct gctcgagcac cgtcgcccca
     1321 cgccccccgt attcacgcgg ccacgagacg gccgcccatc cggcgtcggc catcgtgcgg
     1381 ttccacgcgc gcagcagctc gaatgtcgcg tcgtcgcgtc cggagggccg gcgagcggcc
     1441 accagctctg gggtcaggtt tccggacaac cagtcacgca gctcggcgcg gaactgttcc
     1501 acctcggctg ggtatgagaa gtccacgtgc ccttcttgac gtgttgtcgc cctctgcgcg
     1561 attgactgcg gccaagccgg actttacgtt ggaacagata tttggtcaaa cagcagtgtg
     1621 aagaaggacg gcttggcgag cgaccagcac gaccccgatc gcctggcgcg actgggcatg
     1681 ctggcttcgg ccctgtccgg ccgaacgctg caggtggcgg ccgtccgacc tggcgagccg
     1741 tcgtggacgg acggcgccac gatctttgtc gacgccggcg cgcagcgtca gctcgagacg
     1801 ctggccgtgc aggcctcgct gctggcggcg ggaagcctgg agccgaagct gctgcgcgtg
     1861 ctcaagcggc gccccggggc cactcggcgc taccttgccg tggaaggccg ccgcgcgctg
     1921 gcgcaaaacg cggacgtact gccgccagcc gcgggcacgc tcgtcgacag cggccttgcc
     1981 gtcaccgccg attcccccgc cgcctcgctg cacatggcgc tgagccgtca ggcgatcccc
     2041 gaccctcccg actggttcgg aaccatccac gcccgcaatc tgttggcggc gaacaatatc
     2101 cgcagcaagg cggccgacgg ggccggggcg cgccggcgac atgcccagac gcccgcgcaa
     2161 ttcgacgacc acgacgccgc cgccgcgagt caagacgcgg acatcttcac cagcccggtg
     2221 ggcggtcggg gcgcactggg ccggctgttg ggcagcatgc tgcgggcggt ccgtcgtctg
     2281 ggcgagggcg gccatccggg cgcggacgcg ccgacgcacc ggacgttttc gggaatccgc
     2341 gccggcacag cagccctgtc gaccgcaccg gtgggcccat tcgacgacct ggccgctgac
     2401 ggtgacggga tcacctaccc cgagtgggac attcatcggc ggtgttatcg gccgaactgg
     2461 tgcacggtcc gtgaagtgga gcccgtcgtc cgagacgtcg ggtcgccgga tgtcccgcac
     2521 gggtacggac tgcgacggcc actggcccgt ctgggactgg ggccggattg gtttcaccga
     2581 caggtgtatg gcgacgacat cgacatcgac gccgccgtcg aagcgcgggt cgcggcggcc
     2641 gcgggctcgc tgccggagga agccgtttac attgcgccgc ttcgccgccg gcgcgatctc
     2701 gccgtgctgc tgctgctgga cgtctcgggc tccggcaacg aaccgggcgc acacggcggg
     2761 acggtccacg aacagcaacg ggccgccgcg gcggccttag ccgtttctct gcacgccctg
     2821 ggggatcgct tggcgctcta cgctttccat tcgcagggtc gatcgtcggt ccacgtgatg
     2881 ccggtcaagc gtttcgatga cgacatgaat gcgtcggtgt tgcgacgact gtacggcctg
     2941 acgcccggtg cgtattcgag gctgggagcc gcgattcggc acggggccgc ggtcttggag
     3001 agccggggtg gcacgtcgcg ccggctgctc gtcgtgctgt ccgacggatt ggcctacgac
     3061 cacggctacg aacgggtgta cggggcggcc gatgcccgcc gcgccctcgc cgaagcacgc
     3121 ggtcgcggcg tcggatgcgt ctgcctgacc atcggtgccg gcaccgatcc cgacgagttg
     3181 cagcgcgtgt tcggcagcgc cgcccacgcc accatcgccc gcccggagga actgagccgg
     3241 gtggtcgggc ccttgttccg cacggcgctg cgcgccgccg aggtcctccg tcccgccgct
     3301 cgctccagct tgagccaaat atctgttccg cgctacgctg cgcgcagcac gtgaaagggg
     3361 gatgtccgat ggcggtggag ggcgtggtac ccagggcgca gccgttccgt cccttctatg
     3421 tcacggtcgg cgacgaggag aaggtgttca aggctgcgtt ccgccaaggg ctttcggtgt
     3481 tactcaaggg ccccaccgga tgcgggaaga cgcggttcct ggaggccatg gcgtatgacc
     3541 tggggcggcc cctgatcacg gtggcgtgcc atgacgatct caccaccgcc gatctcgtcg
     3601 ggcgctatct gctgcgcggc ggcgaaacgg aatgggttga cggcccgctg accagggcgg
     3661 tgcgcgaggg cgcgatctgc tacctcgacg aggtggtgga ggcccggcag gacaccacgg
     3721 tggtgctgca tcccctggcc gaccaccgcc gccagctccc cctggagcgg ctcggcgtga
     3781 ccctcgatgc cgccccgggg ttctgcctgg tggtgtccta caaccccggc tatcaaagcg
     3841 tgctcaagga tctcaaggat tcgacccggc agcggatggt ggcgatcgag ttcggctttc
     3901 ccgcggccga cgtcgaagag aagatcgtcg cccatgaggc cggcatcaac gccgaaacgg
     3961 ccgccgagct ggtgcgattg gcgcaggcga tccgccgcct ggagaacggc gggttgcggg
     4021 aggtggcctc gacgagggtg ctcatcgccg cgggacggct catcgccgaa gggttgacca
     4081 ggtcgcaggc cgcgcgcgcc gccatcgccg ggccgttgac cgacgacccg cacgccgccg
     4141 ccggactatc ggaaatgatc gacgcctatc tcctcgacta gggcgggcga ccggcgagac
     4201 ttcgtccgca taactcggcg ctggagcgca agcgatgatt gacggtccga catccccacg
     4261 atagggtcag aacaaatttt aggtttgcgc tgacatgccc gacgcacgag gtagcgggag
     4321 gtagattgtc ttacgaaagc accgcggagc cgatcaggat cggctacctg tttgacttcc
     4381 tgctgccgca gggatacccg caggaaatgc gggacgacct cacccggcca ttcgacctgg
     4441 tgttcaacga cggcgtgcgc gagcgggtga tcgaccgacc ggtaaggatc gtgtaccgag
     4501 aagtcgaggg gctgcccaag ggcaccgtca aggcggtcat cgacgcgtac ggcgaactcg
     4561 tcgacgaagg ctgcctcgtg gtgttcgggc cgcatatcac cgagaacgcg gtcccgttgc
     4621 gggaggcgat cgaggagcga ttccgtgttc cggcgatcaa cgtgtgtggc agtgacgact
     4681 ggctggggga gtggaccttc gcgtttccgc aagggtcgat gaccgacgag ccgatcttct
     4741 ggaccgacct gctggccaag ggtggccaca cgcaagtcgg cgtgctggtc gagcaatccc
     4801 tggtcggcga gagttacctg aagaatttcc gaaacgcctg tcgccgcaag ggcatccgca
     4861 tcgtcgccga ggcgcaggtc gcccagacgg ctcaggacgt cggaacggcg atccgcagcc
     4921 tgcacgaggc caaaccgagt gcgctggtgc actgcggctt cggcttcggc gtcgtgttca
     4981 tcaatccggc tctggcagaa ctggactggg acccgccccg gttcaccagc actgcgtttc
     5041 agaacgcctg gatcaatccc gttatgtggc aggcattcat gggctggacc ggaatcgatc
     5101 agtacgacga aggcaatggc gtgggccagg ctttcctgga ccgctacgcg tcggaatacg
     5161 gacgccgccc gcaatactgc gtgccggtgg tgaaccacga cgtggccaac gcgctgctgc
     5221 acgcgttcgc cgatgcccat cccttgagtc cgcgtggcgt gaaggaagcg ctggagcggg
     5281 tgaagatgct gccggccgcc gcgggcgcac ccgggaccag ggtgtcgttc ggaaagtgga
     5341 cccgccgggc ctggatgggc gccggctacc tggtggcccg ccagctggac gtcgacggcg
     5401 tgagttccca ccttgtcgac cgtttcggtg aggaatgacc gatgaccacg aagcagacaa
     5461 caccttcgac accttcgaca ccttcgacac cttcgacaaa agagtcgcct acccgaccga
     5521 agaagcggtc cgtctgggcc atagtttgta tcctgctcgc cgccgcggca ctcgcggtgg
     5581 tcgcgcacta cgcgcgtacg ggcgcggtct cggaccggat tcggaacccg aacgtcgtcg
     5641 gcgccccgcg gccggtcgag ccgctgttcg gctatcagca ttggcttggt ctgttccaga
     5701 tcttcaccat catctcgatg gccatcgtca tcgcggtcta cgtggtggtg tggcgccggc
     5761 atcccgcgca cccaatcctg ttgatgggca tcgtcacaac gttgatcgtc tggcaggacc
     5821 cgatcatgaa ctgggcgccg tacgcggtgt acaacccgca actgtggcac tggccggagg
     5881 actggccgct ggtgtccctt tcgccgacgg tcgagccctt cctggtcatc ggctacatca
     5941 tgttctatct ggggccctac tttccggcca tctggatcct gcgcaagatt caggcccgtc
     6001 ggcccgtcga ctcgttcgtc tggcggcacc cgttgatcac cctggcggcg atcatcctgc
     6061 ccgtcggcat cgtcatggac gcgatgctgg agacgacgct ggtgcgcacc ggcttttaca
     6121 tctacgacca agtcatcccg ttcgggtccg tattcaccgg caagacatac caattcccgt
     6181 tcatctggga aaccgtgatg gtgacgtttg tgatgatccc ggcgggggtg ctgctgtacc
     6241 gcgatgacac cggtcgcacg gtcgccgaga agctcgctca gcgagcccga attttccccg
     6301 gccggccgac gctggcgatg tttgcggtca tgttcgtcct gatcaacctg gcctacttcg
     6361 cctacgggac cggcttcgcg atcatcaagt ggacaaaaac cgccacctcg gtggcctgcc
     6421 cttggccgta tcccgaggcc aaagtctatg acccacaagg cttctacgag agaaacggtc
     6481 agcccggccc gtactcggtc ggcatctggt cgacctggat gagtgcccaa cccaacgggc
     6541 gcccgaacgt cgaactgcct gccgacggcg gaaggtgcgc gccgcgcaca tgagcgagcc
     6601 tcgcaccatc gtcatcaccg gcgcgtcccg cgggcttggc ctcgcatccg cggcacacat
     6661 gtaccaacgg ggctggcgtg tcgtggcggc catgcgctca cccgagtccg gcctttcggc
     6721 gctgcgcgcg aagaccggcg ccgcagaaga cgatccgcga ttggtcgcag tcccgttgga
     6781 tctcaccgac ccggcgtcga tcgcggccgc ggccaaggcg atcgaggcgg ccgccggggc
     6841 cccgcacgcg ctggtgcaca acgccgggat ctccgctgcg ggagcgc
//
LOCUS       DASSGQ010000345        13023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_360815,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000345 DASSGQ010000000
VERSION     DASSGQ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13023
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360815"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VFI55_15260"
     CDS             <1..918
                     /locus_tag="VFI55_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HET7075420.1"
                     /translation="DYVLLHISDTHLVSDGSLYGAVDADGRLGELLEQFKHSGVRPDA
                     IVFTGDLADTGEPEAYRKLRAVVEPFADELGAELVWVMGNHDDRAALRSWLLDEAPSM
                     APLDRVCIVDGLRIITLDTSVPGHHYGEISKSQLDWLAGELEVPAPNGSILALHHPPI
                     PSVLDLAVTVELRDQAALGRVLRGSDVRAILAGHLHYSTNATFVGIPVSVASATCYTQ
                     DLTVAAGGTRGRDGAQACNLVHVYQDTVVHSVIPLGAGRTVGTFVSPAQAQRKITESG
                     MFIEPSARDSLFRHPPMVLTPSVRRTPVD"
     gene            complement(863..2461)
                     /locus_tag="VFI55_15265"
     CDS             complement(863..2461)
                     /locus_tag="VFI55_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stealth family protein"
                     /protein_id="HET7075421.1"
                     /translation="MAKLVSNGGGRPARRPLAPIVVTRQGKIARLESSLTPHEARIED
                     LVFIRKVLTRADIPHLLIRSHKGRPILAINIELREAVQRALVAACATEPMYAKTTDEK
                     GLSPVLVAKGQLSALADPRILRLYRRRIAPGGLRYGPAFGVELQFWAFEDTAIQCPIE
                     NSLTRKMLPRNELKPATVKLYGYKWPTIAGMFTPHASDVTFDVDMVFSWVDGSDPEFQ
                     ARRASQMSQYVAGEGDDAEARIRQIDELKYALRSVNMFAPWVRRIFIATDSRPPAWLA
                     EHPKITVVRAEEHFSDPTALPTYSSHAVESQLHNIPELSEHFLYSNDDMFFGRPLKAS
                     MFFSPGGITRFIEAKTRIGLGANDPARSGFENAARVNRQLIFERFGQVITRHLEHTAV
                     PLRKSVLREMEREFPDDFARTQASQFRSSTDISVTNSFYHYYALMTGRAVQQEKAKVL
                     YVNTTTREGLALLLVLRKKRGYDFFCLNDSSFPEVSAAERADRVISFLDRYFPIPAPW
                     EKVTADVSRPGFAEPMVSTPLEGA"
     gene            2785..3174
                     /locus_tag="VFI55_15270"
     CDS             2785..3174
                     /locus_tag="VFI55_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol carrier family protein"
                     /protein_id="HET7075422.1"
                     /translation="MAARQRADPAKTRQAVLAIAGWLRDESDAAPERDALATAVRLTA
                     RTLAAVAPGRSVEVRIPPFVAVQCVSGPVHTRGTPPNVVETDPRTWLLLATGLLSFEA
                     AKTTGSLRLSGSRAAEIEQWLPLIAVS"
     gene            3313..4848
                     /gene="purF"
                     /locus_tag="VFI55_15275"
     CDS             3313..4848
                     /gene="purF"
                     /locus_tag="VFI55_15275"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET7075423.1"
                     /translation="MAVAEPEASEHDLNSPREECGVFGVWAPGEDVAKLTYYGLYALQ
                     HRGQEAAGIAVADGSQVLVFKDLGLVSQVFDEQTLAAMPGHVAIGHCRYSTTGDTTWE
                     NAQPVFRNTAAGTGVALGHNGNLVNTAELAMRARAAGLLGTRCPAPATTDSDILGALL
                     AHGAADSTLERAALELLPTVRGAFCLTFMDEATLYACRDPYGVRPLSLGRLDRGWVVA
                     SETAALDIVGASFVRDIEPGELLAIDADGVRSTRFANPMPKGCVFEYVYLARPDSTIA
                     GRSVHGTRLEIGRRLARECPVAADLVIGVPESGTPAAVGYAQESGIPYGQGLMKNAYV
                     GRTFIQPSQTIRQLGIRLKLNPLREVIRGKRLIVVDDSIVRGNTQRALLRMLREAGAL
                     EVHVRIASPPVKWPCFYGIDFPSPAELIANAVEDEEEMLEAVRHAIGADSLGYISLRG
                     MVAASEQPASRLCTACFDGKYPIELPRESALGKNVIEHMLANAARGAGLEDLTSDEVP
                     IVR"
     gene            4914..6008
                     /gene="purM"
                     /locus_tag="VFI55_15280"
     CDS             4914..6008
                     /gene="purM"
                     /locus_tag="VFI55_15280"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET7075424.1"
                     /translation="MTDPGKSPGRDPGSQGITYASAGVDIEAGERAVDLFKPLATRAT
                     RPEVRGGLGGFAGLFALRGDYREPVLAASTDGVGTKLAVAQAMDKHDTVGLDLVAMVV
                     DDLVVCGAEPLFLQDYIAVGRTVPERLSAIVGGIAEGCVRAGCALLGGETAEHPGLME
                     PDHYDLSATGVGVVEADDVLGPDRVKPGDVIIAMGSSGLHSNGYSLARKVLLEIDRMN
                     LAGYVEEFGRTLGEELLEPTRIYAKDCLALAAETHVRTFCHVTGGGLADNLQRVIPHG
                     LVAEVDRGTWTPAPVFNMIAQRGRVSREEMEKTFNMGVGMIAVVAPEDTDRARAMLTA
                     RHLDCWELGTVCKGGKEGPRAKLVGQHPRF"
     gene            complement(6074..6268)
                     /locus_tag="VFI55_15285"
     CDS             complement(6074..6268)
                     /locus_tag="VFI55_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3073 domain-containing protein"
                     /protein_id="HET7075425.1"
                     /translation="MGRGRAKAKQTKVARELKYSSPQTDFQRLQRELSGTGAEESGEL
                     DDSDDPGPEDSWDDPDDWRR"
     gene            complement(6416..7510)
                     /locus_tag="VFI55_15290"
     CDS             complement(6416..7510)
                     /locus_tag="VFI55_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HET7075426.1"
                     /translation="MNAVPAPDPGPDAGAVWHYGDPLGEQRAGETDAVLVDRSHRAVL
                     TLTGKDRQTWLHSISTQHVSGLPEGASTHNLSLDGQGRVEDHWIQTELGGTSYLDTEP
                     WRGEPLLEYLRKMVFWSEVTPDSADLTVLSLLGPRLADRTVLDVLGLDGLPAESIAVP
                     LAGGGFARRMPGAPVSQIELDLVIPRAESPGWQARLAEAGVRSAGVWAYEAHRVAALR
                     PRLGVDTDERTIPHEVGWIGGPGEGAVHLDKGCYRGQETVARVHNLGRPPRMLVLLHL
                     DGSVDRPSTGDAVLAGGRAVGRLGTVVEHVDLGPVALALLKRGLPADTELVTGPKAEV
                     AAVIDPDSVPSAGHIGAGRLAVERLRGGAR"
     gene            7702..8568
                     /locus_tag="VFI55_15295"
     CDS             7702..8568
                     /locus_tag="VFI55_15295"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HET7075427.1"
                     /translation="MIVTLDGAIQPPGTPLLHADDLAAVRGDGIFETLLVRDGGACLV
                     ESHLQRLTQSARSMELPEPDLPRWRHSIAVATKQWVSGTADEGAMRLIYSRGREGGSD
                     PTAYVMVNPVADRVTAARRDGVAAITLNRGLPADGIDAMPWLLAGAKTLSYAVNMAVL
                     RHAARQGAGDVIFVSSDGYILEGPRSTVVIATEAGADGNPCLLTPPPWYPILRGTTQQ
                     ALFEVARANGYDCDFRGLRIADLHAAQGIWLISSMTLAARVHTLDGRRLPRSPMAAEF
                     AELVDAAIVSDR"
     gene            complement(8820..9428)
                     /locus_tag="VFI55_15300"
     CDS             complement(8820..9428)
                     /locus_tag="VFI55_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HET7075428.1"
                     /translation="MAAAAERAKVTASRNIPAFDDLPVPADTANLREGANLNNALLAL
                     LPLVGVWRGEGEGRGADGDYRFGQQIVVSHDGGDYLNWEARSWRLDGTGEYQDRGLRE
                     TGFWRFVDDPDDPSESQAIELLLAHSAGYVELFYGRPRTQSSWELVTDALARSRSGVL
                     VGGAKRLYGIVEGGDLAYVEERVDADGGLVPHLSARLSRFAG"
     gene            complement(9642..9944)
                     /locus_tag="VFI55_15305"
     CDS             complement(9642..9944)
                     /locus_tag="VFI55_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1416 domain-containing protein"
                     /protein_id="HET7075429.1"
                     /translation="MCSAPKQGLTLPASVDLEKETVITGRVVDRDGQSVGGAFVRLLD
                     SSDEFTAEVVASATGDFRFFAAPGSWTLRALSPVGNGDAVVSPDGAGIHEVDVKIA"
     gene            complement(9946..10779)
                     /locus_tag="VFI55_15310"
     CDS             complement(9946..10779)
                     /locus_tag="VFI55_15310"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HET7075430.1"
                     /translation="MARSDVLVSADWAASNLDAANVVFVEVDEDTSAYDTGHIAGAIR
                     LDWRTDLQDPVKRDFVDAQQFSKLLSDRGISNDDTVILYGGNNNWFAAYAYWYFKLYG
                     HDKVKLLDGGRKKWELDGRPLSTDAVSRPPTSYTAEAPDNSIRAFRDEVIAAINAKNL
                     VDVRSPDEFSGKILAPAHLPQEQSQRPGHIPGAINVPWSKAANEDGTFKSDEALATLY
                     ANAGLDGTKETIAYCRIGERSSHTWFVLQELLGHKNVKNYDGSWTEYGSLVGAPIELG
                     S"
     gene            complement(10813..11283)
                     /locus_tag="VFI55_15315"
     CDS             complement(10813..11283)
                     /locus_tag="VFI55_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HET7075431.1"
                     /translation="MPSSNTGLQQDRVDVRGPRFAAWVTTAVLLLTLVVSAASPLAAA
                     VILGVQAVVFAIGAVGGPRRHPYGRVFALVVAPRLGPVKEREPVAPLKFAQLVGLIFA
                     VVGTIGFAVGAFLVGVVATAAALVAAFLNAAFGICLGCQLYPLVARFRANPDPA"
     gene            complement(11503..11934)
                     /locus_tag="VFI55_15320"
     CDS             complement(11503..11934)
                     /locus_tag="VFI55_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET7075432.1"
                     /translation="MTTVIVAIVTALLVATLAGWLLTRRSGQLRETHSESAQGTGSDA
                     VDTADLGLSRSGPTVVHFSAPWCGPCDRVRRVVDQVCEDLGDVAHVEIDLDANPATAQ
                     RFSVLSLPTTLIFDVDGRQRYRASGVPKAADLRTALEPLLA"
     gene            complement(11931..12743)
                     /gene="lmeA"
                     /locus_tag="VFI55_15325"
     CDS             complement(11931..12743)
                     /gene="lmeA"
                     /locus_tag="VFI55_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannan chain length control protein LmeA"
                     /protein_id="HET7075433.1"
                     /translation="MRMRKVLIGVLATLTAVAVLAIGALCVDYGTSIYAEYRLSVNVR
                     KAADLGSDPFVAILAFPFIPQAMRNHYSELEIKANAIDHAMVGKATLEATMHSIDLTY
                     ASWLIKPDAKLPVGKLESRIIIDSMHLGRYMGITDLMVEAPPQETNDATGGTTESGIS
                     GSHGLVFSGTPKSANFDRRVSVSADLAIAPDDQATLIVTPTGIVTGPDTANQTVPDDK
                     RDAVLRAFSARLPNQRLPFGVAPQTVGARGSDVIIEGITYGVNVTLEEFKQS"
BASE COUNT         2163 a         4227 c         4365 g         2268 t
ORIGIN      
        1 gattacgttc tcttacacat cagcgacacc catctcgttt cggatgggtc gctctacggc
       61 gcggttgacg ccgacggcag gctgggcgaa cttctcgagc agttcaaaca ctctggggtg
      121 cgtcccgacg cgatcgtgtt caccggcgat ctggccgata ccggcgagcc ggaggcctac
      181 cgcaagcttc gcgccgtggt ggagcccttc gccgacgagt tgggcgccga actcgtctgg
      241 gtgatgggca accacgacga ccgggccgcg ttgcgtagct ggctgctgga cgaggcaccg
      301 tcgatggcgc cgctcgaccg cgtgtgtatt gtcgacggcc tgcgcatcat cacgctggac
      361 acctcggtgc ccggccacca ctacggcgaa atcagcaagt cccaactgga ttggctcgcg
      421 ggcgaattgg aggtgccggc gccgaacggc agcattcttg ccctccacca cccgccgatt
      481 cccagcgtct tggacctggc cgtcaccgtc gaactgcgcg accaggccgc cctggggcgg
      541 gtgctcaggg gtagcgacgt gcgcgcgatt ttggcggggc acctgcacta ctcgaccaat
      601 gcgacgttcg tcggaatccc ggtttcggtg gcgtcggcca cctgctacac ccaggacctg
      661 acagtggccg cagggggaac gcgtgggagg gacggcgcgc aggcgtgcaa cctggtgcac
      721 gtctaccaag acaccgtcgt gcactccgtg attccgcttg gcgccggaag aaccgtgggc
      781 accttcgtat cgcccgccca ggcgcagcgc aagatcaccg aaagcggcat gtttatcgag
      841 ccgtcggcgc gtgattcgct gttcaggcac cctccaatgg tgttgacacc atcggttcgg
      901 cgaaccccgg tcgactgacg tccgccgtca ccttctccca gggtgcgggg atggggaaat
      961 aacggtccag gaagctgatg acacggtcgg cgcgttcggc agcggagact tcggggaagc
     1021 tgctgtcgtt gaggcagaag aagtcgtaac cgcgtttctt ccgcagcacc agaagtaggg
     1081 cgagaccctc gcgggtagtg gtgttgacgt agagcacctt ggccttttcc tgttggaccg
     1141 cgcggccggt catcagcgcg tagtagtggt agaaggagtt ggtcaccgag atgtcggtgc
     1201 tggaacggaa ctgactcgcc tgggtgcggg cgaagtcgtc ggggaattcg cgttccattt
     1261 ccctcagcac gcttttgcgc aacggaaccg cggtgtgctc caggtgccgg gtgatgacct
     1321 gtccgaaccg ctcgaagatc agctgccggt tcacccgggc cgcgttctca aaaccgctgc
     1381 gcgcaggatc gttggcgccc agcccaatgc gcgtcttcgc ctcgatgaac ctcgtgatcc
     1441 cgcccgggga gaagaacatg ctggccttga gtggccggcc gaagaacatg tcgtcgttcg
     1501 agtacaggaa atgctcgctg agctcgggga tgttgtgcag ttggctttcc accgcatgcg
     1561 agctgtaggt gggcagcgcg gtggggtccg agaagtgctc ctcggcgcgc acgaccgtga
     1621 tcttcggatg ctcggccagc caggcaggcg ggcgcgaatc ggtggcgatg aagatccgac
     1681 gcacccacgg cgcgaacatg ttcaccgatc gcagggcgta tttcaactcg tcgatctgcc
     1741 ggatgcgcgc ttcggcgtcg tcgccttcgc ccgccacgta ttgcgacatt tgcgacgccc
     1801 gtcgcgcctg aaactcggga tcgctgccgt cgacccacga gaaaaccatg tcgacgtcaa
     1861 acgtcacatc gctcgcgtgc ggggtgaaca tccccgcgat ggtcggccac ttgtagccat
     1921 agagtttgac ggtcgccggc tttagctcat tgcgcggcag cattttccgg gtcagcgagt
     1981 tttctatcgg gcactggatc gcggtgtcct cgaaggccca gaattgaagc tccacgccga
     2041 acgccgggcc atatcgcaag ccccccggcg cgattcgccg ccgatacagc cgcaggatgc
     2101 gcgggtcggc caacgcggat agctggccct tggcgacgag gacgggagaa agcccttttt
     2161 cgtcagttgt tttggcgtac atgggctcgg tggcgcacgc agccacgagc gcgcgctgga
     2221 ccgcttcgcg tagttcgatg ttgatggcca gaatcggccg gcccttgtga ctccggatca
     2281 gcaagtgcgg aatgtcggcc cgggtcaaca cctttcgtat gaagaccaag tcctcgattc
     2341 gagcttcatg aggggtcaag ctggattcca ggcgggcaat ctttccttgc cgggtaacga
     2401 cgatgggggc gagcgggcga cgagcgggcc gaccaccccc gttagacacc aacttggcca
     2461 tcggaatcac cgcctcacga tgtcgtagct gaagacgtgg ggagctcgaa cactgagcgc
     2521 aggcgcaggg tgcggccccc ctggccgtcg tgggttatgc gtcaatggtg ccggtgcaac
     2581 gacaccacgt gcaaggcagg cgatttagtg attttccgga catctggagg caacctcgtc
     2641 gttgcaccgg tgtcacgtcg ccttcagtgg ccaagctggc ttcggcaagc gtgcgcggag
     2701 cggaattcgg ttgtcgtggt ttctcttcat ggtttgcagg gtgccccaaa ggcgcgaggc
     2761 tcggtgattt tgagaccctg agacatggcc gcgcgtcaga gagccgatcc ggcgaagacc
     2821 cggcaagcgg tgttggcgat cgcgggctgg ttgcgtgacg agtctgatgc ggcgcccgag
     2881 cgggacgcgc tggcgacggc ggtccggctc accgcacgca ccctggctgc ggtggcgccc
     2941 gggcgcagcg tcgaggtccg gatcccgcca tttgtcgcgg tgcagtgtgt ttccgggccc
     3001 gtgcacacgc gcggcacccc gcccaacgtc gtggagaccg atccgcggac gtggctgctg
     3061 ctggccaccg gactgttgtc gttcgaggcg gcgaagacca ccggttcgct gaggttgtcg
     3121 ggctccaggg ccgctgaaat cgagcagtgg ctgcccctta ttgctgtgag ctgaatatca
     3181 ctgtgaactg caggtttagg gttgcgcgac gtgattttcg gcgctcaaca cgcgggccgg
     3241 attcggcgcg aggcgagttg tgcccgtaga ctccgttacg tcaccaaccg ttccccaggg
     3301 agcccccaaa ccgtggccgt cgctgaacca gaagcatccg agcacgacct gaactccccc
     3361 cgtgaagagt gtggcgtttt cggggtctgg gccccaggtg aggatgtcgc caaactcacc
     3421 tattacggcc tatatgccct gcagcatcgc gggcaggaag ccgccggtat cgccgtcgcc
     3481 gacggatccc aggtgctggt cttcaaagac ctcggcctgg tcagtcaggt gttcgacgag
     3541 cagacgctgg cggcaatgcc gggccatgtc gctataggac actgccgcta ctccaccacg
     3601 ggtgacacta cctgggaaaa cgcgcagccg gtgtttcgca acaccgcggc gggcaccggg
     3661 gtcgcgctgg ggcacaacgg gaatctggtc aacaccgctg aacttgccat gcgtgctcgc
     3721 gccgcggggt tgttgggcac ccgatgcccg gccccggcaa cgacggactc agacattctg
     3781 ggcgccctgc tggcccacgg tgcggccgat tccaccctgg agcgggcggc gctcgaattg
     3841 ctgccgaccg tgcgcggcgc gttctgcctg acgttcatgg acgaggccac gctgtatgcg
     3901 tgccgcgacc cgtatggcgt gcggccactt tcgctgggac gactggatcg cggctgggtg
     3961 gtggcgtccg aaacggccgc gctcgacatc gtcggcgcct cgttcgtccg cgacatcgag
     4021 ccgggcgaat tgctggcgat cgacgccgac ggcgtgcgct cgacgcggtt cgccaacccc
     4081 atgcccaagg gttgtgtctt cgagtacgtc tacctggcac ggccggacag cacgatcgcg
     4141 ggccggtccg tgcacggcac ccggctggag atcggtcgcc ggttggcccg tgaatgcccc
     4201 gttgctgccg acctggtgat cggtgtgccg gaatcgggca cccccgccgc ggtcgggtat
     4261 gcgcaggagt ccggcatccc ctacgggcag ggcttgatga agaacgcgta cgtcgggcgc
     4321 accttcattc agccgtcgca gaccatccgc cagctcggca tccgactgaa gctgaacccg
     4381 cttcgcgagg tgatccgtgg caaacggctc atcgtcgttg acgattcgat cgtgcggggc
     4441 aacacccagc gcgcattgtt gcgaatgctg cgtgaggccg gtgccctcga agtgcacgtg
     4501 cgcatcgcct cacccccggt gaaatggccg tgcttttacg gcatcgactt cccgtcgccg
     4561 gccgagttga tcgccaacgc cgtcgaagac gaggaggaga tgctcgaggc cgtgcggcac
     4621 gccatcggcg ccgactcgct gggttacatc tcgctgcggg gcatggtggc ggcctccgag
     4681 cagccggcat cgcggctgtg caccgcgtgc ttcgacggca agtatccgat cgagctgccg
     4741 cgcgagtccg cgctgggcaa gaacgtcatc gagcacatgc tggccaacgc cgcgcgcggc
     4801 gccgggctgg aagacctgac gtccgacgaa gtaccaattg tgcgctgacg gattgcgccc
     4861 gccgtgcaat gttcgatacc cgcggtgagc gccgcccggt agcctttatc gcgatgacgg
     4921 atcccggaaa aagccccgga cgagacccgg gcagccaggg catcacctac gcgtcggcag
     4981 gcgtggacat tgaagccggt gagcgcgccg tcgatttgtt caagccgctg gcaaccaggg
     5041 ccaccagacc cgaggtgcgc ggcggcctgg gcgggttcgc cgggttgttc gcgctgcgcg
     5101 gcgactaccg cgaaccggtg ctggccgcgt ccaccgatgg cgtcggcacc aagctggcgg
     5161 tcgcccaggc tatggacaaa cacgacaccg tcggcctgga cctggtagcc atggtggtcg
     5221 acgacctcgt ggtctgcggc gcggagccgc tgttcctgca ggactacatc gccgtcggcc
     5281 ggaccgtgcc agaacgcctg agcgccatcg tcggcggcat cgccgagggt tgtgtgcgcg
     5341 ccggctgcgc gctgctcggc ggcgagaccg cggaacaccc tggcctgatg gaaccggacc
     5401 attacgacct gtcggccacg ggtgtcggtg tcgtcgaggc cgacgacgtg ctggggcccg
     5461 accgtgtcaa acccggtgac gtcatcatcg cgatgggctc ctccggcctg cattccaacg
     5521 gatactcgct ggcccgcaag gtcctgctgg agatcgaccg gatgaacctg gccggctatg
     5581 tggaggagtt cggccggact ctgggcgaag agctgttgga acccacccgg atttacgcca
     5641 aagactgcct ggcgctggcc gccgaaaccc atgtccgcac gttctgccac gtcaccggtg
     5701 gcgggctggc agacaacttg caacgcgtca tcccgcacgg gctggtcgcc gaggtcgacc
     5761 gcggcacgtg gacgcccgcg ccggtgttca acatgatcgc ccagcgtggc cgcgtctcgc
     5821 gcgaggagat ggagaagacg ttcaacatgg gcgtcggcat gatcgctgtc gtcgcgcccg
     5881 aagacaccga ccgggcccgg gccatgctga ccgcgcggca cctggactgc tgggagttgg
     5941 gcacggtctg caaaggcgga aaagaaggtc cacgggcgaa actggtcggc cagcacccga
     6001 gattctgaag cccgggaatt aggcgggact agcagtgtgc cgatgcgaag cgcatcggca
     6061 cgtgggggcg gttctagcga cgccagtcgt ctgggtcgtc ccaagaatcc tctgggcctg
     6121 gatcgtccga gtcgtccagc tcaccggatt cctcggcgcc cgtccctgac aactcgcgct
     6181 gaagccgctg gaagtcggtt tgcggggaac tgtatttgag ttctcgtgca accttggtct
     6241 gctttgcctt agcccggccg cggcccatgg gggaaccccc tcgcgcaata acggagcggc
     6301 ctaacaagta ggcggctccg atctcttggt ctcgttattg tcctgccgac agtttaccgt
     6361 gccttacggc cgggcgtcgg ctccccccgc ccgctgtggc ggacctcatc gccgattatc
     6421 tcgcaccgcc ccgcaaccgt tcaacggcta accggcccgc gccgatgtgt ccggcggacg
     6481 gcacggaatc ggggtcgata accgcggcga cctcggcctt cgggccggtt accagttcgg
     6541 tgtccgccgg cagtccgcgc ttgagcagcg ccagtgccac cgggccgagg tcgacgtgct
     6601 cgaccacggt tcccagccgc ccgacggcgc gaccgccggc gagcaccgcg tcacccgtcg
     6661 agggccggtc caccgagcca tcgaggtgca gcaacaccag catccggggc ggtcttccca
     6721 ggttgtgcac ccgggcgacg gtctcttgtc cgcgatagca gcccttgtcc aggtgcacgg
     6781 ctccctcgcc ggggccaccg atccaaccca cctcgtgtgg aatcgtgcgc tcgtcggtgt
     6841 cgacgccgag gcgcgggcgc agcgcggcca cccggtgggc ttcatacgcc cacactccgg
     6901 ccgaccgcac gcccgcctcc gccaggcgcg cctgccagcc gggcgactcg gcgcgcggta
     6961 tcaccaggtc gagttcgatt tggctgacgg gggcgccggg catccgccgc gcaaagccac
     7021 caccggcgag tggaaccgcg atcgactcgg cgggcaaacc gtccaggccc agcacgtcca
     7081 gcacggtcct gtcagccagc cgggggccca gcagcgacag caccgtcagg tcggcgctgt
     7141 ccggggtgac ctcggaccag aacaccatct tgcgcaggta ttccagcagc ggctcacccc
     7201 gccacggttc ggtatccaga tagctggtgc cgccgagctc ggtctggatc cagtggtcct
     7261 cgacccggcc ctggccgtca aggctgaggt tgtgcgtgct ggcgccctcg gggagtccgc
     7321 tcacgtgttg ggtggagatg ctgtgcagcc aggtctgccg gtctttgccg gtcagggtga
     7381 gcaccgcgcg gtgcgagcgg tcgacgagca cggcatcggt ttcgcccgcg cgctgctcgc
     7441 ccagcggatc gccgtaatgc cagaccgcgc cggcgtcggg gccgggatcg ggtgcgggga
     7501 ctgcgttcat gtgcgccgct cctctccccc gcaagcggga ggtaccccca catcgctgcg
     7561 ctctgcatcg tcgcaggcgc agttcacaca tcaactgtac ggacgcccga cgactctgcg
     7621 gccggccgca cgttggccgg cgactgtgcg cccagccgca cagcggccgc tattgtgtcg
     7681 tccatgtccg gccagatggg tgtgatcgtc acgctggacg gcgcgatcca gccgccagga
     7741 acaccgttgt tgcacgccga cgacctggca gcggttcgcg gcgacggcat cttcgagaca
     7801 ctgctggtcc gcgatggcgg ggcctgcctg gtcgagtcgc acctgcagcg actgacgcag
     7861 tcggccagat cgatggagct ccccgagcca gacctcccgc gttggcggca ctcgattgcc
     7921 gtcgccacga agcagtgggt ctcgggtacc gccgacgaag gcgcgatgcg cttgatctat
     7981 agccgtggcc gcgagggcgg ttcggacccg accgcctacg tgatggtcaa ccccgtcgcc
     8041 gaccgagtga ccgcggctcg gcgcgacggg gttgcggcga tcacgttgaa ccgcggattg
     8101 cccgctgacg gtatcgacgc gatgccgtgg ctgctggccg gcgccaagac gctgtcctac
     8161 gcggtcaaca tggccgtttt gcgccacgcc gcccggcagg gtgccggcga cgtaattttc
     8221 gtcagctccg acggctacat tctggaaggc ccacgctcga cggtggtgat cgccaccgag
     8281 gccggagccg acggcaatcc ctgcctgctg acacccccgc cgtggtaccc gatcctgcgc
     8341 ggcaccacgc aacaggccct cttcgaggtg gcccgggcca acggctacga ctgcgacttt
     8401 cgcgggctgc ggatcgctga tctgcatgcc gcacaaggga tttggctgat atcgagcatg
     8461 accctggcgg cgcgcgtgca cactctcgat ggccggcgac tgcctcggtc tccgatggcc
     8521 gccgaattcg ccgagctggt ggacgccgcc atcgtcagcg atcggtgagc gccgaattct
     8581 gttgtgggct cgcagaatgg cgggtacggt cggccgtaca cagggaagga ggtggtccgc
     8641 gaaattgata gcttcatgga catgtgaggt ggctacgcgc tagctgcaaa ggctcggaag
     8701 agccggcgat ctacgcgcgc tggcgaattc cccgtagcca cccggccccc gagcttccgg
     8761 tctgtccaac caggagcttt gctcgggggc cgctccatgt cacgacaccc gcggggccgc
     8821 tatccggcga accgcgacaa ccgcgccgag aggtggggga ccaggccgcc gtcggcgtcg
     8881 acccgttcct cgacgtaggc aagatcgccg ccctcgacga tgccgtagag gcgcttggcg
     8941 ccgccgacaa gcacgcccga ccgactgcgg gccaaggcgt cggtcaccag ttcccacgac
     9001 gactgggtgc gcggccgtcc gtagaacaac tcgacgtaac cggccgagtg ggccaacagc
     9061 aactcgatgg cctgcgactc gctcggatcg tcgggatcgt cgacgaaccg ccaaaagccc
     9121 gtctcgcgca ggcctcgatc ctggtactcg cccgtgccat cgagccgcca agaccgggct
     9181 tcccaattca ggtagtcgcc gccgtcgtgc gagaccacga tctgctgtcc gaaccggtag
     9241 tcgccatcgg ccccgcggcc ctcgccttcc ccacgccaca caccaaccag cggcagcagg
     9301 gccaacagcg cgttattcaa gttggcgccc tcgcgcaggt tcgcggtgtc agcgggaacc
     9361 ggcagatcgt cgaaggcggg gatgttgcgg gacgcggtca ccttggcgcg ttcggccgcg
     9421 gcggccatcg cacggtcgcc taagtccgca gattccaccg gcccgtcgag atcctcgtct
     9481 gaggtcacga ctcgtcagtg atgagccgat agagcgtgta gagcgcaaac catgaaatga
     9541 ccacgactgc taagaccagg aggatctcga agaagagcac cacgggcatg agtgtattcg
     9601 cccacgggct gtccggggac catcccggtc agtgtgatct gtcaggcgat ctttacgtcg
     9661 acttcgtgaa tgcccgcgcc gtccggggac accacggcat cgccgttgcc gaccggggac
     9721 agtgctcgca gcgtccaaga tcccggcgcg gcgaagaacc ggaagtcacc ggtggccgac
     9781 gcgaccacct cggccgtgaa ttcgtccgac gagtccagca gccgaacgaa cgcgccgccc
     9841 accgactgcc cgtcgcggtc cacgacgcgg ccggtgatca ccgtttcctt ctccagatca
     9901 acgctggcgg gcaacgtcag tccttgcttc ggtgcagagc acatatcagc ttcccaactc
     9961 gatcggggca cccactaggg agccgtattc cgtccaactg ccgtcgtagt tcttgacgtt
    10021 tttatgcccg agtaattcct gcagaacgaa ccaggtatgc gacgaacgct ctccgattcg
    10081 gcagtacgca attgtttctt tggtgccgtc caatccggcg ttggcgtaga gcgtggccaa
    10141 cgcctcgtcg gacttgaacg tgccgtcctc attagcggcc ttgctccacg ggacgttgat
    10201 cgcgccgggg atgtggccgg gccgctggct ctgttcctgt ggtaggtgcg ccggggcgag
    10261 gatcttgccg gagaactcgt cgggggagcg cacgtccacc aggttcttgg cgttgatggc
    10321 cgcgatgacc tcgtcccgga aggcgcggat gctgttgtcc ggcgcttcgg cggtgtagga
    10381 ggtcggcggc ctgctgacgg cgtcggtgga cagcgggcgc ccgtcgagct cccacttctt
    10441 gcggccaccg tcgagcagct tgaccttgtc gtggccgtac agcttgaaat accagtaggc
    10501 gtaggcggcg aaccagttgt tattcccgcc gtagaggatg acggtgtcgt cgttggaaat
    10561 gccccggtca gacagcagct tggagaattg ctgggcgtcg acgaagtcgc gcttgaccgg
    10621 gtcctgcaaa tcggtccgcc agtccagcct gatcgcgccg gcgatgtggc cggtgtcgta
    10681 agcgctggtg tcctcgtcga cttctacgaa gacgacgttc gcggcgtcca gattgctcgc
    10741 agcccagtcc gcggagacta ggacgtcgga gcgtgccatg gttggaatcc tttcggttgc
    10801 ttctgacgag agttaggcgg gatccgggtt cgcgcggaag cgcgcgacca gcgggtagag
    10861 ctggcagccc aggcagatgc cgaaggccgc attgaggaat gcggccacca gcgcggccgc
    10921 cgtggcgacg acgccgacca agaaggcgcc gacggcgaat ccgatggtgc cgacgaccgc
    10981 gaagatcaga ccgacgagtt gagcgaactt caggggtgcg accggttcgc gctccttgac
    11041 gggtccgagc cggggtgcca cgacgagggc gaacacccgg ccgtagggat ggcggcgtgg
    11101 gcccccgacg gcgccgatgg cgaataccac ggcttgaacg ccgagaatga cagcggccgc
    11161 cagcggactg gcggccgaaa cgacgagcgt gagcaggagg actgcggtgg tgacccaggc
    11221 ggcgaacctg ggtccacgaa cgtcgacgcg gtcctgctgc aggccggtgt tgctgctcgg
    11281 cacgctatta ctcctaggaa ctgtcatttg cgcggaagac ggatggaggc atgccgcgga
    11341 agggctccga agctgctccg agtgcacgta aaggcgcggc tactcagcag ctacagcaac
    11401 aacaacaacc cgcggtgcgg cacagatcga ctgcgcgacg cttggtgagc gagggctcca
    11461 ggcgggttga cacgtcggca agcttaccca ataacgcagt gctcaagcca acagcggttc
    11521 gagcgcagtg cgcaggtcgg ctgccttggg caccccggac gcccggtatc gctgtcgccc
    11581 gtcgacgtca aagatcaacg tggtgggcag cgagagcacc gaaaatcgct gtgcggtcgc
    11641 cgggttggcg tccagatcga tctcgacgtg ggccacgtcg cccaggtcct cgcagacctg
    11701 atcgaccacc cggcggactc ggtcgcacgg tccgcaccac ggggcgctga agtgcacgac
    11761 ggtcggtccg ctacgagaca ggcccagatc cgcggtgtcg accgcgtcgc ttccggtgcc
    11821 ttgagccgac tccgaatgag tttcccggag ctgtcctgag cggcgggtca gcaaccatcc
    11881 cgccagggtt gccacgagga gcgccgtcac tatcgcgacg ataacggtcg tcatgactgc
    11941 ttaaactctt caagcgtgac gtttactccg taggtgatgc cctcgatgat gacgtccgag
    12001 ccacgcgctc ccacggtttg gggcgccacc ccgaacggaa gcctctggtt gggcagcctg
    12061 gcgctgaatg cccgaagcac cgcgtcgcgc ttgtcatctg ggacggtttg gttggcggtg
    12121 tcgggtccgg tgacgatgcc ggtaggcgtg acgatcaacg tggcctggtc gtcgggggcg
    12181 atggcgaggt ctgccgaaac gctgacacgg cgatcgaagt ttgccgattt cggcgtgccg
    12241 ctgaacacca gcccgtggct gccggagatc ccggattcgg tggtgccgcc cgtcgcgtcg
    12301 ttggtttcct ggggtggcgc ctcaaccatc aggtcggtga tgcccatata ccggcccaaa
    12361 tgcatcgagt cgatgatgat ccggctctcc agtttgccca ccggcagctt cgcgtcgggc
    12421 ttgatcagcc acgacgcgta cgtcaggtcg atcgagtgca tcgtggcttc gagggtggcc
    12481 ttgccgacca tcgcgtgatc gatggcattg gccttgatct ccagctcgct gtagtgattg
    12541 cgcatcgcct gcggaatgaa cgggaacgcc aggatcgcga cgaacgggtc ggaacccagg
    12601 tccgccgcct tgcgcacgtt gaccgacagc cggtattcgg cgtaaatgct ggtcccgtag
    12661 tcgacgcaaa gggcaccaat ggcgagcacg gccacagcgg tcagcgtcgc cagcacaccg
    12721 atcagtacct tgcgcatccg catattgtgg cgcacttgcc gccggactcc ttcgcatcgc
    12781 tcctcccgca ccgcccgccg cagctcccga agcgcatcgt cgccacgggg gtttggattg
    12841 cgtggcttct cacacttcgg cccgaagcgc atcgtcgcca caccgaaaaa gcaggtggca
    12901 cgttatcgtt agatgacctg gcaactaacg tctcattgcg tcgtgaattt gggagatgcc
    12961 tttcccacga gctggaaggg gcttgttgga gttattactg ctgacctcgg agctacatcc
    13021 tga
//
LOCUS       DASSGQ010000346        11091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_25656, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000346 DASSGQ010000000
VERSION     DASSGQ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11091
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25656"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VFI55_15330"
     CDS             complement(<1..683)
                     /locus_tag="VFI55_15330"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HET7075434.1"
                     /translation="MTRITTGELRDAVLDEGSFLSWDRELVAVPISKSYERELAEARA
                     ATGMDEAVLTGEGRLFGRRVAVVVCEFGFLGGSIGVAAAERITAAVERATAERLPLLA
                     SPSSGGTRMQEGTVAFLQMVKITAAVRLHKQAHLPYLVYLRNPTTGGVFASWGSLGHV
                     TVAEPRALIGFLGPRVYQLLYGEPFPPGIQTAENLQQHGVIDGIVALDELRHTLDRAL
                     RVMADPPEPL"
     gene            711..1445
                     /locus_tag="VFI55_15335"
     CDS             711..1445
                     /locus_tag="VFI55_15335"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET7075435.1"
                     /translation="MIGVTQAEAVMTIELQRPKRRNALNSELVDELREAIQKAGDDGS
                     TRAIVLTGQGTVFCAGADLSGDAFAADYPDRLIELHKAMDAAPVPVIGAINGPAIGAG
                     LQLAMQCDLRVVAVDAFFQFPTSKYGLALDNWSIRRLSSLVGHGRARAMLLTAEKLTA
                     EVALQTGMANRIGTLPDAQAWAAEVAGLAPLAIQHAKRVLNDDGAFEEPWPLHKELFD
                     KAWGSQDVIEAQVARIEKRPAKFQGA"
     gene            1451..2569
                     /locus_tag="VFI55_15340"
     CDS             1451..2569
                     /locus_tag="VFI55_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7075436.1"
                     /translation="MLRGALRLAAGTASLTAGGWVLRALRGAPAALGASPESIRAVAQ
                     GSPNYSDGQFVNLDPASAVKIDREEMRRIAWELVGGRGGSLPAAPIPLAAPQIFETDP
                     STLAVSWFGHSTALLEIDGYRVLTDPVWSNRCSPSDIVGPQRLHPPPVQLEGLPAVDA
                     VVISHDHYDHLDLDTIIALARTQRAPFFVPLGVGAHLRGWGIPEDRIVELDWHQSAQV
                     DDLTLICVPARHFSGRFFDRDNTLWASWVFVGPTRRAYFGGDTGYTKSFTQIGADHGP
                     FDLTLLPIGAYNTAWPDVHMNPEEAVRAHLDITDPGSGLLVPVHWGTFRLAPHPWAEP
                     VERLLAAATPAQVDVAVPQPGQRIDPAAPRRFNPWWRL"
     gene            2622..4238
                     /locus_tag="VFI55_15345"
     CDS             2622..4238
                     /locus_tag="VFI55_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET7075437.1"
                     /translation="MTKRAARAAVVALLVVLTGCASGQPASSPPPATSDAPPNEVSGL
                     AIPAGRIDDAIAKVDGLVGDLMKRSGIPGMTVAVVHGGKTVYAKGFGVRDASKGDGPD
                     NKVDADTVFQLASISKSVGATVVAHEVSDNVVTWDTPVISKLPWFALNDPYVTSHVTI
                     ADLFSHRSGLPDHAGDKLEELGYDRRQTLERLKYSPLAPFRISYAYTNFGVTGAAEAV
                     AAAAGKPWEDLSDEALYRPLGMASTSSRFADFVARPNHAVNHVKVGDKWEPRFQRDPD
                     PQSPAGGVSSSVNDMARWLIMLLGNGTYHGQRVASPEALSPANTAEVISAPATTPKAR
                     SGFYGYGFNVSVTSSGRAEYSHSGAFALGAATNFVVLPSEDVGIIALTNAAPLGVPET
                     LTAEFMDLVQYGQIREDWANLYQQAFDAMNKPEGSLVGKQAPANPAPARPLSDYTGGY
                     ANDYWGPAEVTERDGALQLAMGPKNQMFPLTHWDGDTFTFPVTGENAPPGTISKAVFS
                     GFPRTTLNLEYFDLGKDLDNDSDRLGTFTR"
     gene            4235..6676
                     /locus_tag="VFI55_15350"
     CDS             4235..6676
                     /locus_tag="VFI55_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET7075438.1"
                     /translation="MTLANTLGLTDADVAQRVAEGKSNAVPELATRSVAAIIRANVFT
                     RINAILSVLLVIVLATGSLINGLFGLLIVANSVIGMVQEIRAKQTLDKLAIVGQAKPV
                     VRRQSGTRTLPPGEVVLDDIIELGPGDQVVVDGEIVEAENLEVDESLLTGEADPIDKV
                     GGDAVMSGSFVVAGVGSYRATKVGPEAYAAKVAGEASKFTLVKSELRNGINQILQFVT
                     YLLVPAGLLTIYTQLFSTHAGWRQSVLRMVGALVPMVPEGLVLMTSIAFAVGVIRLGQ
                     RQCLVQELPAIEGLARVDVVCADKTGTLTENGMRVSDVEKVAGLAAQSETVADVLASL
                     AAADARPNASMQAIGETYHRPPGWIATATAPFKSATKWSGVSYRDHGNWVIGAPDVLL
                     DPGSAAAERAERIGAQGLRVLLLGRGELPVDHPGAPGRVTPVALVVLEQRVRPDAAET
                     LDYFAAQDVSVKVISGDNAVSVGAVAGKLGLRGETMDARQLPTDPAQLAEVLESYTTF
                     GRVRPDQKRSIVHALQSHGHNVAMTGDGVNDVLALKDADIGVAMGAGSPASRAVAQIV
                     LLDNRFATLPYVVGEGRRVIGNIERVANLFLTKTVYSVLLALFVGTECLFSKWLKADP
                     LLYPFQPIHVTIAAWFTIGIPSFILSLAPNNERAHHGFVRRVLTSALPSGLIVGVATF
                     ASYLVAYHGRHATFQQQEQASTAALITLLMSALWVLAVVARPYEWWRVALVVASGAAY
                     VVIFSIPLARKEFLLDPSNAAVTSTALGIGVLGAAAIEATWWIRAKMLGVQPRLWREP
                     AGSVRATSVAGAPAS"
     gene            6738..6926
                     /locus_tag="VFI55_15355"
     CDS             6738..6926
                     /locus_tag="VFI55_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003404724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HET7075439.1"
                     /translation="MGFVDKAKDLLSQNAEKVEAAIDKAGEFVDEKTQGKFSDTIHKV
                     QEEAKKVTGSAGKDDQQN"
     gene            6931..7404
                     /locus_tag="VFI55_15360"
     CDS             6931..7404
                     /locus_tag="VFI55_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7075440.1"
                     /translation="MAKLSGSIDVPLPPEEAWQHASDLSRYKDWLTIHRVWRSALPKE
                     IEKGTIVESIVEVKGMYNRIRWTVVRYKPPEGMTLNGDGVGGVKVKLMAKVTPKNDGS
                     VVSFDVHLGGPALFGPIGMIVAAALRSDIRESLQNFVTVFTRPDPSLNGDRGTGR"
     gene            7388..8635
                     /locus_tag="VFI55_15365"
     CDS             7388..8635
                     /locus_tag="VFI55_15365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019666026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HET7075441.1"
                     /translation="MAPVADRLADLVRATVGVDLPVRIRAWDGSEAGPTDDPAGPLLV
                     VRSRRALRRLLWCPGELGLARAYVTGDIDVEGDLTDGLRRAWRVSGSRRDDTVSQRGW
                     AVAALRAVGLGAVGLPPSPPASEARLSGRLHTLRRDRAAIAHHYDLSNDFYQLLLDDH
                     MAYSSAYFTRQGQSLHDAQTAKLDMICRKLDLKAGAQLLDVGCGWGSLILYAAQRYGV
                     RATGITLSARQRDFIVARAAERGLTDSVEVHLQDYRDFGKLPTSRAAFDGVASIEMGE
                     HVGERLYPTYVEILHRALRPGGRLLLQQMSRRADAAPGGGAFIEAYIAPDMHMRPLWQ
                     TLRYLQDAGFEIRSVAAMREHYARTAEHWMNTLESRYDEFVALLGDEIARVWRLYLAG
                     GRLAFEEGRMGVDQIAAEKAGAR"
     gene            8718..9491
                     /locus_tag="VFI55_15370"
     CDS             8718..9491
                     /locus_tag="VFI55_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7075442.1"
                     /translation="MPIRDGAPLGAPCWIDLTTSDVERAQDFYNTVFGWTFESAGPEY
                     GGYVNAAKDGHRVAGLMANRPEFGSPDNWSTYFLTADINATMSAVTAAGGSVCLETME
                     VPAKGYMGLVADPSGAFFGLWQPLEHRGFEVIGEAGSPVWHQLTTRDYPAAVDFYREV
                     LGWRTEQISDTDEFRYTTAWFGDQQLLGVMDGAGCLPDGVPSNWTIFFGAEDVDKTLQ
                     VIADNGGAVVRGAEDTPYGRLAAATDPTGVIFNLSSLQS"
     gene            9562..9996
                     /locus_tag="VFI55_15375"
     CDS             9562..9996
                     /locus_tag="VFI55_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075443.1"
                     /translation="MTRRLHPGWLVALFALLISASAWMPWLTTAANGGGWASAVGGTH
                     GSLVLPPGFGAGQLIVLLSSTLLVAGAMVGRGLSMRLASVAALVISLLIIALTVWYYK
                     INVHPPVSAEYGLYVGAVGAVCALVCSATAVIGGVASRRSSP"
     gene            complement(10001..10480)
                     /locus_tag="VFI55_15380"
     CDS             complement(10001..10480)
                     /locus_tag="VFI55_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7075444.1"
                     /translation="MDKKKRRHTHWPSRLASIGAIRFARRSSNFEETVRFYRELVGLP
                     LYETFEASYGSNGAIFGLPSWNLTLEIVESVEPVTVDHQEQLCLYFPDIEAQQAAIAR
                     LQDAGMKAVQQHPYWDANGAVTYRDPDGREVVFAPFVFGVNEPGASAASGKHEFPSA"
     gene            complement(10559..>11091)
                     /locus_tag="VFI55_15385"
     CDS             complement(10559..>11091)
                     /locus_tag="VFI55_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HET7075445.1"
                     /translation="WEKLFRFLALDRLQARLHRWRFNLVTGAVQEEQLSESVTEFGTI
                     NADYAAGSYRYTYAATGKSGWFLFDGLVKHDLLTGGQHRVSFGDGVYGSETAMAPRVG
                     GTGEDDGYLVTLTTDMNTDASYCVVFDAARIADGPVCTLLLPERISSGTHSTWAPGHR
                     LRRWHTAESAASTIGL"
BASE COUNT         1822 a         3607 c         3780 g         1882 t
ORIGIN      
        1 agcggctcgg gaggatcggc catcacccgc agcgcgcggt ccagcgtgtg gcgcagctcg
       61 tcaagcgcga caatgccgtc gatcaccccg tgctgctgta ggttctcggc ggtttggatg
      121 cccggcggga acggttcgcc gtacagcagc tggtacaccc ggggcccgag gaagccgatc
      181 agagcgcgcg gctctgcgac ggtgacgtgg cccagcgaac cccacgaggc gaacaccccg
      241 ccggtggtcg ggttgcgcaa gtagacgagg tagggcaggt gtgcctgctt gtggagcctg
      301 accgccgcgg tgatcttcac catctgcaaa aacgcgacgg tgccctcctg catgcgggtt
      361 ccgcccgagc tcggcgaggc cagcaacggc aggcgctcgg cggtcgcccg ctccacggcg
      421 gcggtgatcc gttcggccgc cgccaccccg atcgagccgc cgaggaagcc gaattcgcag
      481 accaccacgg ccacccggcg cccgaacagg cgcccctcgc cggtcagcac cgcttcgtcc
      541 atgccggtgg cggcgcgggc ctcggccagt tcccgctcgt aggacttgga gatgggcacc
      601 gccaccagtt cgcggtccca gctgaggaaa gacccttcgt cgaggaccgc atcgcgcagc
      661 tctccggttg tgatacgagt cacggaacga ggctatatag ggtgggttgc atgatcggtg
      721 tcacccaggc tgaagccgtc atgaccattg agctgcagcg cccgaagcgc cgcaatgccc
      781 tgaattccga gcttgtcgac gagctgcgtg aggccatcca gaaggccggc gacgacgggt
      841 caacgcgcgc gatcgtgctg accggccagg gcacggtgtt ctgcgccggc gcggatctgt
      901 ccggagacgc gttcgccgcc gactatcccg atcggctcat cgagttgcac aaggcgatgg
      961 atgcggctcc ggtgccggtg atcggggcca tcaacggccc cgccatcggc gccggcttgc
     1021 agctggccat gcaatgcgat ctacgcgtcg tcgcggtcga cgccttcttc cagtttccga
     1081 cctcgaaata cggtcttgcc ctggataatt ggagcatccg cagactgtct tcgctggttg
     1141 gtcacgggcg ggcccgcgcg atgttgctga ccgcggagaa gctcaccgcc gaggtggcgt
     1201 tacagaccgg gatggccaac cgaatcggta cgctgcccga cgcgcaggcc tgggcggccg
     1261 aggtagccgg cctggcgccg ctggcgatac agcacgccaa gcgggtgctc aacgacgacg
     1321 gcgcctttga ggaaccctgg ccgctgcaca aggagctgtt cgacaaagcc tggggaagcc
     1381 aggatgtcat cgaagcccag gtcgcgcgga tcgagaagcg acccgccaag ttccaggggg
     1441 cctgaccgtc gtgttgcggg gtgcgctaag actggccgcc ggcacggcat cgttgacggc
     1501 cggcggctgg gtgctgcgcg cactgcgcgg ggcgccggct gccctcggtg cgtctccgga
     1561 gtcgatccgg gcggtggcgc agggctcgcc caactactcc gacggccagt tcgtcaacct
     1621 cgacccggcc tcggcggtca agatagaccg cgaggaaatg cggcgcatcg cgtgggagct
     1681 cgtgggtggg cgcggcggaa gcctgccggc agcgccgatt ccgctggccg ccccgcaaat
     1741 cttcgaaact gaccccagca cgctcgcggt cagctggttc ggccactcga cggcgctgct
     1801 ggagatcgac ggttaccggg tgctcaccga tccggtgtgg agcaaccgct gctcgccgtc
     1861 ggacatcgtc ggaccgcagc gcctgcatcc gccgccggtg cagctggagg ggctgcccgc
     1921 cgtcgatgcg gtggtcatca gccacgacca ctacgaccac ctcgacctcg acaccatcat
     1981 cgcgctggcc cgcacacagc gggcgccgtt cttcgtgccg ctcggcgtcg gcgcgcacct
     2041 gcgcgggtgg ggaataccgg aagaccgcat cgtcgagctg gactggcatc agagcgctca
     2101 ggtcgacgac ctcaccttga tttgcgtgcc cgcgcggcac ttttcgggcc ggttcttcga
     2161 ccgcgacaac accctctggg cgtcgtgggt ctttgttggt ccgactcgtc gcgcctattt
     2221 cggcggcgac accggctaca ccaaaagctt cacgcaaatc ggtgccgacc acggaccgtt
     2281 cgacctgacc ctgttaccca tcggggcgta caacacggca tggcccgacg ttcacatgaa
     2341 tcccgaggag gcggtgcggg cgcatctgga catcaccgac ccggggtcgg ggctgctggt
     2401 gccggtccac tggggcacgt tccggctggc tccgcacccg tgggctgaac cggtcgagcg
     2461 cctgctggcg gccgcgaccc ccgctcaggt cgacgtggcg gtgcctcaac ccggtcaacg
     2521 catcgatccc gccgcgccgc gcaggttcaa cccgtggtgg cggctctgac tctcgccagc
     2581 tgttcgggcc gggtgggcag cgcgcgctaa agtttgcggc catgacgaaa cgcgcggcaa
     2641 gggccgcggt cgttgcgctc ttggttgtgc tgactggatg cgcgtccggg caaccggcat
     2701 ccagcccacc gcccgcaacg tccgacgcgc cacccaacga agtctcgggc ctcgcgatcc
     2761 ccgccggtcg catcgatgat gccatagcaa aagtcgacgg cctggtcggc gacctgatga
     2821 aacgcagcgg cattcccggc atgaccgtag ccgtcgtcca tggcggaaag acggtctatg
     2881 ccaagggttt cggcgtcagg gacgcaagca agggtgacgg gccagacaac aaggtggacg
     2941 ccgataccgt cttccagttg gcatcgattt cgaaatcggt gggcgccacg gtggtggcac
     3001 atgaagtcag cgacaacgtc gtcacgtggg acacgccggt gatctccaag ctgccgtggt
     3061 ttgcactcaa cgatccatac gtcactagcc atgtcaccat cgccgacctg ttctcccatc
     3121 gttccgggct gcccgaccac gccggtgaca aactggagga actgggctac gaccgccggc
     3181 agacgctcga acggctgaag tactcaccgt tggcgccttt tcgaatcagc tacgcgtaca
     3241 ccaacttcgg ggtgaccggc gccgcggagg cggtggcggc cgcggcgggc aaaccgtggg
     3301 aggacctgtc cgacgaggcg ctgtaccgcc cactgggcat ggcgtccacg agttcacgat
     3361 tcgccgactt cgtcgccagg cccaaccatg cggtcaacca cgtcaaagtc ggtgacaaat
     3421 gggagccgcg ctttcaacgc gatccggatc cgcagtcgcc cgcgggcggg gtgagttcgt
     3481 cggtcaacga catggcgcgc tggctgatca tgctgctggg caacggaact taccacggcc
     3541 agcgcgtcgc gtcgccggag gcgctgtcgc ccgccaacac cgcggaggtc atctcggcgc
     3601 cggcgaccac gccgaaggcg cggtccggtt tctacggcta cggcttcaac gtgtcggtga
     3661 cctcgtcggg acgcgccgag tacagccatt ccggtgcttt tgcgttgggg gctgcgacga
     3721 acttcgtcgt gctgccatca gaggacgtcg gcatcatcgc actgaccaac gcggcacccc
     3781 tcggcgtgcc ggagacgctg accgccgaat tcatggatct ggtgcaatac gggcagattc
     3841 gcgaagactg ggccaacctg taccagcagg cgttcgacgc gatgaacaag ccggagggct
     3901 cgttggtggg caagcaggcg ccggccaatc cagcgccggc ccgaccgctg agcgactaca
     3961 ccggcgggta cgccaatgac tactggggtc cggccgaggt gaccgaacgc gacggcgcgt
     4021 tgcaactggc gatggggccg aaaaaccaga tgttccccct tacccactgg gacggtgaca
     4081 ccttcacgtt cccggtgacg ggcgaaaacg cgccgcccgg aacgatttcc aaggccgtct
     4141 tctccgggtt cccgcgtacg acgctgaacc tggagtactt cgacttgggc aaggacttgg
     4201 acaacgactc cgacaggctg ggaaccttca cccgatgaca ctcgccaata cattgggttt
     4261 gaccgatgcc gacgtggcgc agcgggtcgc cgaaggcaag agcaacgccg ttccggaact
     4321 cgcgacccgc agcgtcgcgg cgatcatccg ggccaacgtc ttcacgcgga tcaacgcgat
     4381 cctcagcgtg ctgctggtga tcgtgctggc gaccggttcg ctgatcaatg ggctgttcgg
     4441 cttgctcatc gtcgccaata gcgtcatcgg aatggtccaa gagattcggg ccaagcagac
     4501 gctggacaag ctcgcgatcg tcgggcaggc gaaaccggtg gtgcgcaggc aatccgggac
     4561 gcggacgctg ccgcccggcg aggtcgtcct cgacgacatc atcgagctcg gtccgggcga
     4621 ccaggtcgtc gtcgacggcg agatcgtcga ggcagagaac ctcgaggtcg acgagtcgct
     4681 gctgaccggc gaggccgacc cgatcgacaa ggtcggcggt gacgcggtga tgtccggcag
     4741 ctttgtggtc gccggcgtcg gctcctaccg cgccaccaag gtcggcccgg aagcgtacgc
     4801 ggccaaggtc gcgggggagg ccagcaagtt caccctggtg aaatccgaac tgcgcaacgg
     4861 tattaaccag attctgcagt tcgtcaccta cctgctggtg ccggccggtt tgctgacgat
     4921 ctacacccag ttgttcagca cacacgcggg ttggcggcag tcggtgctgc ggatggtcgg
     4981 cgcgctggtg ccgatggtgc ccgagggcct cgtgctgatg acatcgatcg cgttcgcggt
     5041 tggcgtcatc aggctcggtc agcgtcaatg cctggtccag gaattgcccg ccatcgaggg
     5101 attggcccgg gtcgacgtgg tgtgcgccga caagaccggc accttgaccg agaacggcat
     5161 gcgggtttcc gatgtcgaga aggtcgcggg actggcggcg caatccgaaa cggtcgccga
     5221 cgtgttggcc tcactggccg cggccgacgc ccgccccaac gccagcatgc aggcgatcgg
     5281 cgagacctat caccggccgc cgggctggat cgccactgcc acagcacctt tcaagtccgc
     5341 caccaagtgg agcggggtgt cctaccgcga tcacggcaac tgggtgatcg gtgcgcccga
     5401 tgtgctgctc gatccgggat cggcggccgc cgaacgggcc gagcggatcg gcgcccaggg
     5461 gctgcgggtg ctgctgttgg gccgtggcga gctgcccgtg gaccaccccg gcgcgccagg
     5521 tcgggtcacc ccggtcgcgc tggtggtgct cgagcagagg gtgcgccccg acgccgctga
     5581 gacattggat tacttcgccg cacaggatgt ttcggtcaag gtgatctccg gcgacaacgc
     5641 ggtatcggtc ggtgccgtcg cgggcaagct cggactgcgc ggcgagacga tggacgcgcg
     5701 ccaactcccc accgacccgg cccaactggc tgaggtgctg gagtcctaca ccaccttcgg
     5761 ccgggtgcgg cccgaccaga agcggtccat cgtgcatgcg ctgcaatcgc acgggcacaa
     5821 cgtggcgatg accggagacg gtgtcaacga tgtgctggcc ctcaaggacg ccgacatcgg
     5881 tgtcgcgatg ggtgccggca gcccggcgtc gcgcgcggtg gcgcagatcg ttttgctgga
     5941 caacaggttt gccacgctgc cgtatgtcgt cggcgaaggc cggcgggtga tcggcaacat
     6001 cgagcgggtc gccaacctgt ttctcaccaa gacggtttat tcggtgctgc tggcgctgtt
     6061 tgtgggtacc gaatgcttgt tttccaaatg gctcaaggct gaccccctgc tgtacccgtt
     6121 tcagccgatc cacgtcacga tcgcggcgtg gttcacgatc ggaatcccgt cgttcatcct
     6181 gtcgctggca cccaacaacg agcgggctca ccacggcttc gtgcggcggg tgctgacctc
     6241 cgcgttgccc tcggggctta tcgtcggcgt cgcaaccttc gcctcctacc tggtggctta
     6301 ccacggccgt cacgccacgt tccagcaaca ggaacaggcg tcgaccgcgg cgctgatcac
     6361 actgctgatg tccgcgctct gggtgctcgc tgtggtggcg cgcccctacg agtggtggcg
     6421 agtggcgctg gtggtggcgt ccggcgcggc ctacgtggtg atattcagca tcccgttggc
     6481 ccgaaaggag ttcctgctcg acccgtcgaa cgcggcggtc acctcgaccg cgctgggaat
     6541 cggcgtgctg ggcgccgcgg ccatcgaggc gacgtggtgg atccgggcca agatgttggg
     6601 cgtgcaaccg cggttgtggc gcgagccggc cgggtcggtc cgggcgacta gcgtggccgg
     6661 ggcgccggca agttagggtt ctaccagcca agtaggtttc cccaggcgta ctggggaacg
     6721 tcggggaagg accgcgcatg ggattcgtgg ataaggcaaa agacctgttg tcgcagaacg
     6781 ccgagaaggt cgaggcggcg atcgacaagg ccggcgagtt cgtcgacgag aagacacagg
     6841 gcaagttctc cgacaccatc cacaaggtgc aggaagaggc caagaaggtc accggctcgg
     6901 ccggcaagga cgatcagcag aactgagcgc atggcgaaac tctccggatc catcgacgta
     6961 ccactgcccc ccgaagaggc gtggcagcac gcctccgatc tgtcccgtta caaggactgg
     7021 ctgaccattc accgggtctg gcggagcgcg ttgcccaaag agatcgagaa gggcacgatc
     7081 gtcgagtcca tcgtcgaggt caagggcatg tacaaccgga tcaggtggac ggtcgtgcgc
     7141 tacaagccgc cggagggcat gacgctcaac ggcgatggcg tcggcggtgt caaggtcaag
     7201 ctgatggcca aagttacgcc caaaaacgac ggctccgtcg tcagctttga cgtacacctc
     7261 ggtggcccgg cacttttcgg gccgatcggg atgatcgtcg cggccgccct gcgcagcgac
     7321 atccgcgaat ccctgcagaa cttcgtcacc gtgttcaccc gacccgatcc gagcctcaac
     7381 ggggatcgtg gcaccggtcg ctgaccgcct ggcggacctg gttcgcgcca ccgtcggcgt
     7441 ggacttgccg gtgcggatcc gcgcctggga cggcagcgag gcgggcccca ccgacgaccc
     7501 ggccggcccg ctgctggtgg tgcgctcgcg tcgcgcgttg cgtcgattgc tctggtgtcc
     7561 aggtgaactc ggcctggcgc gggcctatgt gaccggcgac atagatgtcg agggtgacct
     7621 gaccgacggt ctgcgacggg cgtggcgcgt ttccggatcg cggcgggacg acaccgtgtc
     7681 gcagcggggc tgggcggtgg cggcgttgcg cgcggtgggg ctgggtgcgg tagggctgcc
     7741 gcccagccct ccggccagcg aggcgcggct gtcgggccgg ctgcataccc tgcggcgcga
     7801 tcgggcagcg atcgcgcacc actacgacct gtccaacgat ttctaccagc tgctgctcga
     7861 cgaccacatg gcgtactcgt cggcgtattt cacccggcaa ggccagtcgc tgcacgacgc
     7921 gcagaccgcg aagttggaca tgatctgccg caagctcgat ctcaaagcgg gggcgcagct
     7981 gctggatgtc ggttgtgggt ggggatcact gatcctgtac gccgcacagc gttacggtgt
     8041 gcgcgctacc gggatcacgc tgtcggcacg gcagcgcgat ttcatcgttg cccgtgccgc
     8101 tgaacgtggc ctgaccgaca gtgtcgaggt acacctgcag gattaccgcg atttcggcaa
     8161 gctcccgacc tcgcgggcgg cctttgacgg ggtggcttcc atcgagatgg gcgagcacgt
     8221 gggggagcgg ctgtatccga cttacgtcga aatcctgcat cgcgcattgc ggccgggtgg
     8281 ccggctgctg ttgcagcaga tgtcgcgccg cgccgatgcg gcaccgggtg gaggggcatt
     8341 catcgaggcc tatatcgcgc ccgatatgca catgcggccg ctctggcaga cgctgcgcta
     8401 cctacaggac gcagggttcg agatccgttc ggtcgcggcg atgcgtgagc actacgcgcg
     8461 taccgccgag cactggatga acaccctgga aagccgctac gacgagttcg tggccctgct
     8521 gggcgacgaa atcgcgcggg tgtggcgact gtacctggcc ggcggccgcc tcgccttcga
     8581 ggaaggccgg atgggtgtgg atcagatcgc ggcggaaaag gccggcgcgc gatgagtttc
     8641 cggtgcggtt tgagtcgata catgtgaacc ccgatcgcgg ggccgaaaac gccacgacct
     8701 aacaattgga ggccactatg cccatccgtg acggcgcgcc gctgggcgct ccctgctgga
     8761 tcgacctgac aacctcggac gtcgagcgcg cccaggactt ctacaacacc gttttcggtt
     8821 ggacgttcga gtctgccgga cccgagtacg gcggatacgt caacgccgcc aaggacggcc
     8881 accgcgtcgc cggcctgatg gccaaccgcc cagagttcgg gtccccggac aactggtcca
     8941 cctacttcct caccgccgat atcaacgcga ccatgtccgc ggtgaccgcg gcgggtggct
     9001 cggtgtgcct ggaaaccatg gaggtgcccg ccaaggggta catgggtctg gtggccgacc
     9061 cgtcgggcgc gttcttcggg ttgtggcagc ctttggagca ccgcggcttc gaggtgatcg
     9121 gggaggccgg ctctccggtg tggcatcagc tgaccacacg cgactacccc gccgcggtcg
     9181 acttctaccg cgaggtcctc gggtggcgga ccgagcagat ttcggacacc gacgagttcc
     9241 gttacaccac agcgtggttc ggcgaccagc aattgctcgg cgtgatggac ggcgccggct
     9301 gcctgcccga cggtgtcccg tcgaactgga cgatcttctt cggcgccgag gatgtcgaca
     9361 agacgctgca ggtgatcgcc gacaacgggg gcgcggtggt gcggggggcc gaggacaccc
     9421 cctacggccg gttggccgcg gcgaccgacc cgacgggcgt catcttcaat ctgtcctcgc
     9481 tgcagagctg acccggcggc gccacgccag cgtggcgttc gctcacacgg gcgcggagcc
     9541 aggtcggcta atctcgcaac catgacccgc cgcctgcatc ccggttggct ggtggcgctt
     9601 ttcgcgttgc tcatttcggc cagcgcctgg atgccgtggc tgacgacggc ggccaacggc
     9661 ggcggctggg ccagcgccgt cggaggcact cacggcagtc tggtactgcc gccgggtttc
     9721 ggtgcgggcc agctcatcgt gttgttgtcg tcgacgttgc tggtggccgg ggcgatggtg
     9781 ggccgcggtc tatcaatgcg gctcgcttcc gttgccgcgc tggtcatttc gctgctcatc
     9841 atcgcgctca ccgtgtggta ctacaaaatc aacgtccacc cgccggtgtc agccgaatac
     9901 ggcctctacg tcggtgcggt aggcgcggtc tgcgcgctcg tatgttcggc tacggccgtg
     9961 atcggcgggg tggcttcccg ccgatccagc ccctgaggct tcaggccgac ggaaactcgt
    10021 gcttacctga ggcggcgctg gcgccgggct cgttgacgcc gaatacgaag ggcgcgaaga
    10081 cgacctcgcg gccgtccggg tcgcgatagg tgaccgcacc gttcgcatcc caatacgggt
    10141 gctgctggac ggctttcatc cccgcatcct gaaggcgtgc gatcgcggcc tgctgtgcct
    10201 cgatgtcggg gaagtacagg cacagctgtt cctggtggtc cacggtgacg ggctcgaccg
    10261 actcgacgat ttccagcgtc aggttccagc tgggcaggcc gaaaatggca ccgttactgc
    10321 cgtagcttgc ctcgaacgtc tcgtagagcg gcaaccccac cagttcacgg taaaaccgca
    10381 ccgtctcttc gaagttcgac gagcggcgag caaagcgaat ggcgccgatc gacgccaacc
    10441 gtgagggcca gtgtgtgtgg cgacgctttt tcttatccat tgctactccg attcgccggg
    10501 cccgcagatc tgcaggccgc tactcgtgtt tcctggcgtg tcatccgcgc ggtgaccatc
    10561 acagcccgat ggtgcttgcg gctgactccg ccgtatgcca gcgccgcagc cggtgccctg
    10621 gcgcccatgt cgaatgagtg ccgctggaaa tccgttctgg cagaagaagt gtgcacaccg
    10681 gcccgtcggc gatccgggca gcgtcgaaga ccacgcagta agacgcgtcg gtgttcatgt
    10741 cggtggtcag ggtgaccagg tagccgtcgt cctcgccggt gccgccgacg cgcggcgcca
    10801 tcgcggtctc gcttccgtag acgccgtcgc cgaacgagac gcggtgctgg cctccggtga
    10861 gtaggtcgtg tttgaccagc ccgtcgaaca ggaaccagcc cgatttgccg gtcgccgcat
    10921 aggtgtatcg gtaactgccc gcggcgtagt cggcattgat ggtcccgaat tcggtgacgg
    10981 actccgacag ctgttcttct tgcacggcgc cggtgaccag attgaaacgc cagcggtgca
    11041 gccgcgcctg cagacgatcc agcgccagga accgaaacag cttctcccac t
//
LOCUS       DASSGQ010000347        10956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_996436,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000347 DASSGQ010000000
VERSION     DASSGQ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10956
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996436"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /gene="nuoD"
                     /locus_tag="VFI55_15390"
     CDS             <1..1172
                     /gene="nuoD"
                     /locus_tag="VFI55_15390"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HET7075446.1"
                     /translation="GVLRLILEIEGETVISARCGIGYLHTGIEKNLEYRYWTQGVTFV
                     TRMDYLSPFFNETAYCLGVEKLLGITDQIPERVNVIRVMMMELNRISSHLVALATGGM
                     ELGAMTPMFVGFRAREIILSLFEKITGLRMNNAYIRPGGVAQDLPPNAANDIAEALKE
                     LRQPLRQMGELLNENAIWKARTEGVGYLDLTGCMALGITGPILRSTGLPHDLRRSEPY
                     CGYQNYEFEVITDDRCDAYGRYIIRVKEMWESLTIVEQCLDKLRPGPTMIEDRKIAWP
                     ADLTVGPDGMGNSPEHIAKIMGSSMEALIHHFKLVTEGIRVPAGQVYTAVESPRGELG
                     VHMVSDGGTRPYRVHYRDPSFTNLQAVAAMCEGGMVADLIAAVASIDPVMGGVDR"
     gene            1169..1927
                     /gene="nuoE"
                     /locus_tag="VFI55_15395"
     CDS             1169..1927
                     /gene="nuoE"
                     /locus_tag="VFI55_15395"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HET7075447.1"
                     /translation="MTRPQGQPVFIRLGPPPEEPNQFVVEGAPQSYSPEVRARLEVDA
                     KEIIGRYPNKRSALLPLLHLVQSEDSYLTPAGLEFCGEQLGLTGAEVSAVGSFYTMYR
                     RGPTGDYLVGVCTNTLCAVMGGDAIFDTLKEHLGIGNDETTSDGSITLQHIECNAACD
                     FAPVVMVNWEFFDNQTPQSARELVDSLRAGKPEAPTRGAPLCAFRETSRILAGLPDQR
                     PDQGQGGAGAATLAGLQVAKELGMQAPAAPEGQK"
     gene            1924..3264
                     /gene="nuoF"
                     /locus_tag="VFI55_15400"
     CDS             1924..3264
                     /gene="nuoF"
                     /locus_tag="VFI55_15400"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HET7075448.1"
                     /translation="MTSLQATALTPVISRYWDDPESWTLNTYQRNDGYRGMKKALAMA
                     PDEVIGAVKDSGLRGRGGAGFSTGTKWSFIPQGDTGPAAKPHYLVVNADESEPGTCKD
                     IPLMLATPHMLVEGVIIASYAIRASHAFIYVRGEVLPVLRRLQNAVAEAYAAGYLGRD
                     INGSGFDLDLVVHAGAGAYICGEETALLDSLEGRRGQPRLRPPFPAVAGLYGCPTVIN
                     NVETIASVPAIILNGIEWFRSMGTEKSPGFTLYSLSGHVTRPGQYEAPLGITLRELLA
                     YAGGMRAGHQLKFWTPGGSSTPLLTDEHLDVPLDYEGVGAAGSMLGTKALEIFDETTC
                     VVRAVRRWTEFYKHESCGKCTPCREGTFWLDQIYKRLETGSKTNPATVEDIDKLSDIS
                     DTILGKSFCALGDGAASPVMSSIKHFRDEYVAHVEAGGCPFDPRDSMLAADGKA"
     gene            3272..5704
                     /locus_tag="VFI55_15405"
     CDS             3272..5704
                     /locus_tag="VFI55_15405"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HET7075449.1"
                     /translation="MTVSSGAKPETGDAVSQPTMVTLTVDGVEISVPKGTLVIRAAEL
                     IGIQIPRFCDHPLLDPVGACRQCLVEVEGQRKPMASCTTVATDDMVVRTQLTSPAADK
                     AQHGVMELLLINHPLDCPMCDKGGECPLQNQAMSNGRADSRFADVKRTFAKPINISSQ
                     VLLDRERCILCARCTRFSEQIAGDPFIDMQERGALQQVGIYANEPFDSYFSGNAVQIC
                     PVGALTGTAYRFRARPFDLVSSPSVCEHCASGCAQRTDHRRGKVLRRLAGDDPKVNEE
                     WNCDKGRWAFSYANQPDLITTPLIRDADGSLQPASWSHAIAAAVEVLEANRGRTGVLV
                     GGRATWEDAYAYSKFARITLDTNDIDFRARPHSEEEADFLAARIAGRPLSVSYADLES
                     APVVLLVGFEPEDESPIVFLRLRKAARKNALPVYAVAPFATRALQKMSGRLLKTVPGE
                     EAAALDALATGEIGDLLSTPGAVIMVGERLATAPGALSAAARLADATGARLAWVPRRA
                     GERGALEAGALPGLLPGGRLVADDAARAQVAAGWSVDELPAAVGRDVDGILAGAADGT
                     LGALLVGSVEPDDFADPDAVLAALDAAGFVISLELRHSAVTERADIVFPVAPTTQKAG
                     AFLNWEGRFRDFGPALHGTAQRAGQSDHLILDALADEMGVYLGMSSVEEARQEMSALG
                     NWGGKRPAAPQVTSPNPAQPGAGEAVLTGWRMLLDNGRLQDGEPYLAGTARKPFVRLS
                     ADTAAEIGAADGDDVTVSTPRGSITLPLIVTDMPDRVVWVPLNSPGSAVHRQLGVSIG
                     NLVKIVAIGARI"
     gene            5701..6966
                     /gene="nuoH"
                     /locus_tag="VFI55_15410"
     CDS             5701..6966
                     /gene="nuoH"
                     /locus_tag="VFI55_15410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HET7075450.1"
                     /translation="MTAFGHDTWWLVLGKSLAIFVFLMLTVLVAILAERKLLGRMQLR
                     PGPNRVGPKGALQSLADGIKLALKESITPGGIDKFVFFAAPVISVIPAFTAFAFIPFG
                     PEVSVFGHWTPLQLTDLPVAVLFILGLSAIGVYGIVLGGWASGSTYPLLGGVRSTAQV
                     ISYEVAMGLSFATVFLYAGSMSTSQIVAAQDRVWYVFLLLPSFVIYLISMVGETNRAP
                     FDLPEAEGELVAGFHTEYSSLKFAMFMLAEYVNMTTVSALAATMFFGGWHAPWPMNMW
                     ATANTGWWPLIWFTAKVWTFLFIYFWLRATLPRLRYDQFMALGWKLLIPAALVWVMIA
                     AVIRTMRNQGYPHWTPLLVVTSFVVAVTLVLRLRRPFTPPSIRALELQLRRQRAESSA
                     PSPEITEPAFPTPPLPARKVGASKEKARA"
     gene            6959..7513
                     /gene="nuoI"
                     /locus_tag="VFI55_15415"
     CDS             6959..7513
                     /gene="nuoI"
                     /locus_tag="VFI55_15415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HET7075451.1"
                     /translation="MPKFFDAVAGFGVTFGSMFKKTVTEEYPEKPGPVAPRYHGRHQL
                     NRYPDGLEKCIGCELCAWACPADAIYVEGADNTEEARFSPGERYGRVYQINYLRCIGC
                     GLCIEACPTRALTMTNDYEMADDNRADLIYEKDRLLAPLLPEMTVPPHPRASGATDKD
                     YYQGNVTADGLREPQDGPKTGEAQ"
     gene            7534..8307
                     /locus_tag="VFI55_15420"
     CDS             7534..8307
                     /locus_tag="VFI55_15420"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET7075452.1"
                     /translation="MAANLAQDTMVRTSTGEAVTFWLLGALALIGAVGVVLAVNAVYS
                     AMFLAMTMIILAVFYMIEDAMFLGVVQVVVYTGAVMMLFLFVLMLIGVDSAESLKETL
                     GGQRVAAVVIGIGFGVLLLAGIGNAATGGFVGLTAANANGNVEGLAALIFSRYLWAFE
                     LTSALLITAAVGAMVLAHRERFERRKTQRELSEERFRAGGHPTPLPNPGVYARQNAVD
                     VAALLPDGSYSDLSVSPILRRRGADGKEIPAEAVRGGTS"
     gene            8304..8603
                     /gene="nuoK"
                     /locus_tag="VFI55_15425"
     CDS             8304..8603
                     /gene="nuoK"
                     /locus_tag="VFI55_15425"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET7075453.1"
                     /translation="MNPANYLYLSALLFTIGAAGVLLRRNAIVMFMCVELMLNAVNLA
                     FVTFARIHGHLDGQMIAFFTMVVAACEVVVGLAIIMTIFRARKSASVDDANLLKG"
     gene            8614..10500
                     /gene="nuoL"
                     /locus_tag="VFI55_15430"
     CDS             8614..10500
                     /gene="nuoL"
                     /locus_tag="VFI55_15430"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET7075454.1"
                     /translation="MTHFTWLLVALPLAGAAILLFGGRRTDAWGHLLGCAAALAAFAV
                     GATLLADMLGRSGTDRVIHQKVFTWIPVGQLQVDLGLQIDQLSMCFVLLISGVGSLIH
                     IYSVGYMADDPDRRRFFGYLNLFLASMLLLVVADNYVVLYVGWEGVGLASYLLIGFWY
                     HKPSAATAAKKAFVMNRVGDAGLALGMFLMFSTFGTLSYAGVFGAAPAAGKGALTAMG
                     LLLLLGACAKSAQVPLQAWLGDAMEGPTPVSALIHAATMVTAGVYLIVRSNPLYNLSP
                     DAQLGVVIVGAVTLLFGAFIGCAKDDIKRALAASTMSQIGYMVLAAGLGPAGYAFAIM
                     HLLTHGFFKAGLFLGSGSIIHAMHEEQDMRRYGGLRKALPITFATFGLGYLAIIGVPP
                     FAGFFSKDAIIEAALGVGGVRGYVLGGAALLGAGITAFYMTRVMLMTFFGDKRWAPDA
                     HPHEAPAVMAWPMILLAVGSVFSGFLFAYNGTLQHWLEPVVGAHEEAAHALPAWVSTA
                     LALGVVIVGIAVAYRMYGTAPIPRVAPVRVSALTTAARADLYGDAFNEEVFMRPGAEL
                     THALVEFDDEGVDGSVNRLAALVGRTSNRLRRLQTGFARNYALSMLAGAVLLSALILA
                     VRLW"
     gene            10535..>10956
                     /locus_tag="VFI55_15435"
     CDS             10535..>10956
                     /locus_tag="VFI55_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET7075455.1"
                     /translation="MNGAFLNGVPWLSVLWLVPLAGSLLVILMPPGQRQLAKWTGLFF
                     GLLTLAVSIVITLGFKAGGATYQFVESHRWIPAFGATYSLGVDGIAVVLVLLTAVLIP
                     LLQVAGWNDGVERTRGVQAYVALTLAIESMVLISVVAL"
BASE COUNT         1725 a         3635 c         3685 g         1911 t
ORIGIN      
        1 acggggtgtt gcggctgatc ctcgagatcg agggcgagac ggtcatttcg gccagatgcg
       61 gaatcggcta cctgcacacc ggaatcgaga aaaacctcga ataccgctac tggacccagg
      121 gtgtcacctt cgtgacccga atggattacc tgtcaccgtt tttcaacgag accgcctact
      181 gcctgggtgt ggagaagctg ctcggcatca ccgatcagat accggagcgg gtcaacgtca
      241 tccgggtcat gatgatggaa ctcaaccgga tctcgtcgca tctggttgca ttggccaccg
      301 gcggcatgga gctgggcgcg atgaccccga tgttcgtcgg tttccgggcg cgcgagatca
      361 tcctttccct gttcgagaag atcaccggcc tgcggatgaa caacgcctac atccggcccg
      421 gcggcgtcgc gcaggatctt ccgcccaacg cggccaacga catcgcggaa gccctcaagg
      481 agttgcggca accgctgcgc caaatgggcg agctgctcaa cgaaaacgcc atctggaaag
      541 cccgcaccga gggcgtgggc tacctggacc tgaccgggtg catggcgctg ggcatcaccg
      601 gaccgatcct gcgttccacc gggttgcccc acgacctgcg gcgcagcgag ccctactgcg
      661 gctaccagaa ctacgaattc gaggtgatca ccgatgacag gtgtgacgcc tacgggcgct
      721 acatcatccg ggtcaaggag atgtgggagt cgctgacgat cgtggagcag tgtctggaca
      781 aattgcggcc cggcccgacg atgatcgagg accgcaagat cgcgtggcct gccgacctga
      841 cggtgggccc ggacggcatg ggcaactcac ccgagcacat cgcgaagatc atgggcagct
      901 cgatggaagc gctgatccac cacttcaagc tcgtcaccga gggcattcgc gttccggcgg
      961 ggcaggttta caccgcggtg gagtcacccc gcggcgagct gggcgtgcac atggtcagcg
     1021 acggcggcac ccggccctac cgggtgcact accgggatcc gtctttcacc aacctgcaag
     1081 cggtcgccgc gatgtgcgag ggcggaatgg tcgccgactt gatcgccgcg gtcgccagca
     1141 tcgacccggt catgggcgga gtggaccgat gacccgcccg cagggccaac cggtgttcat
     1201 ccggctcggg cccccgcccg aagagcccaa ccagttcgtc gtcgagggcg ccccgcagtc
     1261 ttactcaccg gaggtgcggg cgcggctgga ggtcgacgcg aaagagatca tcggccgcta
     1321 cccgaacaag cgctcagcgc tgctgccgct gctgcacctg gtgcaatccg aggactccta
     1381 cctcacaccg gcgggtttgg agttctgcgg cgagcagctg ggactgaccg gggccgaggt
     1441 gtccgcggtg ggcagcttct acaccatgta ccgccgcggg cccaccggcg attacctggt
     1501 cggggtctgc accaacacgt tgtgcgcggt gatgggcggc gacgcgatct tcgacaccct
     1561 caaggaacac ctcggcatcg gcaacgacga gaccacctcc gacggatcca tcacgttgca
     1621 acacatcgag tgcaacgccg cctgcgattt cgcaccagtg gtgatggtca actgggaatt
     1681 cttcgacaac cagacgccgc agtcggcgcg cgaactcgtc gactcgctgc gcgcgggcaa
     1741 acccgaggcg cccacccgcg gcgctccgct gtgtgccttc cgcgagacgt cgcgcatcct
     1801 ggccggccta cccgaccagc gccccgacca aggccagggt ggcgcgggcg cagcgacatt
     1861 ggcgggtctg caggtggcga aggaactcgg catgcaggcc ccggccgcac cggaaggcca
     1921 gaaatgacct ccctgcaagc caccgcgttg accccggtga tcagccggta ctgggatgac
     1981 cccgagtcgt ggacgctgaa cacctatcaa cgcaacgacg gctaccgggg gatgaaaaag
     2041 gctctggcca tggcgccgga cgaggtgatc ggcgccgtca aggattccgg gctgcgtggg
     2101 cgcggcggcg cgggcttctc gacggggacg aaatggtcgt tcatcccgca gggcgacacc
     2161 ggcccggcgg ccaagccgca ctacctggtg gtcaacgccg acgagtcgga acccggtacc
     2221 tgcaaagaca ttccgctgat gctggcgacg ccgcacatgc tcgtcgaagg cgtcatcatc
     2281 gcctcctacg cgatccgggc cagccacgcc ttcatctacg tccgcgggga agtgctgccg
     2341 gtgttgcgcc gcctgcaaaa cgcggtggcc gaggcctacg ccgccggtta cctgggccgc
     2401 gacatcaacg gctccggctt tgacctggac ctggtggtgc acgccggcgc gggcgcctac
     2461 atctgcggtg aggaaaccgc gctgctggac tccctggagg ggcgacgcgg ccagccgcgg
     2521 ctgcgcccgc cgttccccgc ggtggcagga ctgtacggct gccccacggt gatcaacaac
     2581 gtcgagacga tcgccagcgt cccggcgatc atcctcaacg ggatcgagtg gttccgctcg
     2641 atgggcaccg agaaatcgcc tggcttcacg ctgtattcgc tgtccggaca cgtcacccgc
     2701 ccgggtcagt acgaggcccc gctcgggatc acgctgcgcg agctgctcgc ctatgccggc
     2761 gggatgcgcg cggggcacca gctcaagttc tggacgcccg gggggtcgtc gacgccgctg
     2821 ctcaccgacg agcacctcga cgtgccgctg gactacgagg gcgtgggcgc ggccggctcg
     2881 atgctgggga ccaaggcgct ggagatcttc gacgaaacca cctgcgtggt gcgcgcggtg
     2941 cgccgctgga ctgagttcta caagcacgaa tcctgcggga aatgcacgcc ctgccgggag
     3001 ggcaccttct ggctggacca gatctacaaa cggctggaaa ccggctcgaa aaccaaccca
     3061 gccaccgtcg aggacatcga caagctgtcc gacatctccg acaccatcct cggaaagtcg
     3121 ttctgcgcgt tgggcgatgg cgccgccagc ccagtcatgt cgtcgatcaa gcacttccgc
     3181 gacgagtacg tcgcgcacgt cgaagcaggc ggatgcccgt tcgacccccg agactccatg
     3241 ctcgctgccg acggaaaggc gtgacgcgcc aatgacggta tcttcagggg ccaaacccga
     3301 aacgggcgac gcggtgagtc agccgacgat ggtgacgctg accgtcgacg gtgtcgaaat
     3361 cagtgttccc aagggaactc tggtgattcg cgcggccgag ttgatcggca tccagatccc
     3421 ccggttctgc gaccacccgc tgctggaccc cgtcggcgcg tgccggcagt gtctggtgga
     3481 agtcgaggga cagcgcaagc cgatggcatc gtgcaccacg gtcgccaccg acgacatggt
     3541 ggtgcgcacc caactcacgt cgccggccgc cgacaaagcc caacacggcg tcatggagct
     3601 gttgctgatc aaccatccgc tggactgccc gatgtgcgac aagggcggcg aatgcccgct
     3661 gcagaaccag gcgatgtcca acggccgagc cgattcccgg ttcgccgacg tcaagcgcac
     3721 gttcgccaag ccgatcaaca tctcgtcgca ggtgctgctg gatcgcgagc gctgcatcct
     3781 gtgcgctcgt tgcacgcgat tctcggagca gatcgccggt gacccgttca tcgacatgca
     3841 ggagcgaggc gcgctgcagc aggtcggcat ctatgccaac gagccgttcg actcgtactt
     3901 ctcgggcaat gcggtgcaga tctgcccggt cggggcgctg acgggcaccg cgtaccggtt
     3961 ccgggcgcgc ccattcgatc tggtctcgag cccgagcgtg tgcgagcatt gcgcctccgg
     4021 ctgcgcccag cgcaccgacc accgccgcgg caaggtgctg cgccggcttg ccggcgacga
     4081 cccgaaagtc aacgaggagt ggaactgcga caagggccgc tgggcgttca gctacgccaa
     4141 ccagccggac ctgatcacca ctcccctgat ccgcgatgcc gacggctcgc tgcagcccgc
     4201 gtcgtggtcg cacgcgatcg ccgccgcggt cgaggtcctc gaggcgaacc gcgggcgcac
     4261 gggtgtgctg gtcggcggca gggcgacctg ggaggacgcc tacgcgtact ccaagttcgc
     4321 ccggatcacg ttggacacca acgacatcga cttccgcgct cgtccgcact cggaggagga
     4381 ggcggacttc ctggcggccc gcatcgcggg ccggccgctg agcgtcagct atgccgactt
     4441 ggaatcggcg ccggtggtgt tgctggtcgg gttcgaaccg gaggacgagt cgccgatcgt
     4501 gttcctgcgg ctgcgcaaag cggcccgcaa gaacgcgctc cccgtgtacg cggtcgcccc
     4561 gttcgccacc cgcgcattgc agaagatgtc cggccggttg ctcaaaaccg tccccggcga
     4621 ggaggctgcc gcgctggacg cgctcgcgac cggtgaaatc ggtgatctgc tgtcgacgcc
     4681 cggcgcggtc atcatggtcg gggaacgctt ggcgacggcg cccggcgcct tgtccgcggc
     4741 ggcccggctc gccgacgcca ccggggcccg cctggcatgg gtgccgcggc gggcgggcga
     4801 gcggggcgcg ctggaggccg gtgcactgcc cgggttgctc cccggtggcc gcctggtggc
     4861 cgacgacgct gcccgcgcgc aggtcgccgc cgggtggagc gtcgacgagt tgcccgccgc
     4921 ggtcgggcgt gacgtcgacg gcatacttgc cggcgccgcc gacggaacgc tgggcgcgct
     4981 gctggtcggc agcgtcgaac ccgacgactt cgccgatccc gacgcggtgt tggccgcgct
     5041 ggacgccgcc ggctttgtga tcagcctgga actgcggcac agcgcggtca ccgaacgcgc
     5101 cgacatcgtg ttcccggtcg cgccgacgac gcagaaggcg ggtgcattcc tgaactggga
     5161 gggccgcttc cgcgacttcg ggcccgcgct gcacggtacg gctcagcgag ccggccagtc
     5221 ggaccacctc attctcgacg cactggccga cgagatgggc gtctacctgg ggatgtcctc
     5281 ggtcgaagaa gcgcgtcagg agatgtcggc gctgggcaac tggggcggga agcgtcccgc
     5341 ggccccgcag gtcacaagcc ccaaccccgc ccagcccggc gcgggcgaag cggtgctcac
     5401 cggctggcgg atgctgctcg acaatggccg cctgcaagac ggcgaaccgt atctggccgg
     5461 taccgcgcgc aaaccgttcg tgcggctgtc ggccgacacc gccgccgaga tcggtgccgc
     5521 cgacggcgac gacgtcacgg tcagcacccc gcgcggatcg atcaccctgc cgctgatcgt
     5581 caccgacatg cccgaccggg tggtgtgggt tccgttgaac tcgccgggtt ccgccgtgca
     5641 ccggcaattg ggtgtgtcta tcggcaacct ggtcaaaatc gtggcgatcg gagcgcgcat
     5701 atgaccgcct tcgggcacga cacctggtgg ctggtgctcg gcaagtcgct cgccatcttc
     5761 gtcttcctca tgctgacggt gctggtggcg attctggccg aacgcaagct gctgggccgg
     5821 atgcagctgc ggcccggccc caaccgggtg ggcccgaagg gcgccctgca gagtctggcc
     5881 gacgggatca agttggcgct caaggagagc atcacacccg gcggcatcga caaattcgtc
     5941 ttcttcgcgg cgcctgtcat ttccgtgatc cccgccttca ccgcgttcgc tttcatccca
     6001 ttcggtcccg aggtgtcggt attcggccat tggacgcctt tgcagttgac cgacctcccg
     6061 gtcgcggtgt tgttcatcct gggcctgtcg gcgatcgggg tgtacggcat cgtgctgggc
     6121 ggctgggcct ccgggtccac ctacccgctg ctgggcggcg tgcgctcgac cgcccaggtc
     6181 atctcctacg aggtcgcgat gggcctgtcc ttcgcgacgg tgttcctcta cgccggctcc
     6241 atgtcgacgt cgcagatcgt ggccgcacag gatcgggtct ggtatgtgtt cctgctgctg
     6301 ccctcgttcg tcatctacct catctccatg gtcggtgaaa ccaacagggc gccattcgat
     6361 ttgcccgagg ccgagggtga gctggtcgcg ggattccaca ccgagtactc gtcgctgaag
     6421 ttcgcgatgt tcatgctggc cgagtacgtc aacatgacga cggtttcggc gctggccgcg
     6481 accatgttct tcggcggctg gcatgcccca tggccgatga acatgtgggc gacggccaat
     6541 accggctggt ggccgctgat ctggttcacg gccaaggtat ggaccttcct gttcatctat
     6601 ttctggctgc gcgccaccct gccccggctg cgctacgacc agttcatggc gttgggctgg
     6661 aagttgttga ttccggccgc gctggtgtgg gtgatgatcg cggcggtgat ccgcacgatg
     6721 cgcaaccagg gttacccgca ttggacgccg ctgctggtgg tcaccagctt tgtggtggcc
     6781 gtgacgctgg tgctccggtt gcgaaggccg ttcaccccgc cgagcattcg cgcgctggaa
     6841 ctgcagcttc gccggcagcg cgccgaaagc tccgcccctt ccccggaaat caccgagccg
     6901 gcgttcccca ccccgccgtt gccggcacgc aaggtcggcg cgagcaagga gaaagcacgt
     6961 gcctaagttt ttcgatgccg tagcgggctt cggcgtgacg ttcgggtcga tgttcaaaaa
     7021 gaccgtcacc gaggaatatc cggaaaagcc cggccccgtc gccccgcgct accacggccg
     7081 tcatcaactc aaccggtacc cggacggcct ggagaaatgc atcggctgcg agctgtgcgc
     7141 ctgggcctgc ccggcagacg cgatctatgt cgagggcgcc gacaacaccg aggaagcgcg
     7201 attctcgccc ggcgaacgct acggccgggt gtaccagatc aactacctgc gctgcatcgg
     7261 atgcggtttg tgcatcgagg cctgcccgac cagggcgctg acgatgacca acgactacga
     7321 gatggccgac gacaaccgcg ccgacctgat ctacgagaag gaccgactgc tcgccccgct
     7381 gctgcccgag atgacggtgc cgccgcatcc ccgggcgtcg ggtgccaccg acaaggacta
     7441 ctaccagggc aacgtgacgg cagacgggtt gcgcgagccc caggacggac ccaaaaccgg
     7501 agaggcccag tgaccgcggc gccggcggtg aacctggccg cgaatcttgc gcaagacacg
     7561 atggtgcgca cctccacggg ggaagcggtg acgttctggc tgctgggcgc actggcgctg
     7621 atcggcgccg tcggggtggt gctggcggtc aacgccgtgt actcggcaat gtttctggcg
     7681 atgacgatga tcatcctggc ggtgttctac atgatcgagg acgcgatgtt tttgggtgtc
     7741 gtccaagtcg tggtctacac cggcgcggtg atgatgttgt tcttgttcgt gctgatgctg
     7801 atcggtgtgg actccgcgga atcgctcaag gagacgctcg gcggtcagcg cgtcgcggcg
     7861 gtggtgatcg gcatcgggtt cggtgttctg ctgctggccg gcatcggcaa tgcggccacg
     7921 ggaggttttg tcgggcttac cgccgccaat gccaacggca acgtcgaagg cctggccgca
     7981 ctgatctttt cgcgctatct ctgggcgttc gagctgacca gcgcgttgct gatcaccgcc
     8041 gctgtcggcg cgatggtgct cgcgcatcgg gagcgtttcg agcgccgcaa gacgcagcgc
     8101 gaactgtcgg aggagcgctt ccgcgccggc gggcatccca ccccgctgcc caacccgggc
     8161 gtctacgccc gccagaatgc ggtcgacgtg gccgcgctgc tgcccgatgg gtcgtattcg
     8221 gacttgtcgg tttcccccat ccttcgacgc cgtggcgcgg acggcaaaga gatccccgcc
     8281 gaggccgtga gaggcggcac gtcatgaatc cggccaatta cctctacctt tcggcactgc
     8341 tgttcaccat cggtgcggcg ggtgtgctgt tgcgccgcaa cgccatcgtg atgttcatgt
     8401 gcgttgagct gatgctgaac gccgtcaacc tggctttcgt cacgttcgcg cggatccacg
     8461 gccatttgga cggacagatg atcgcgttct tcaccatggt ggtggccgcg tgcgaggtcg
     8521 tcgtcggcct ggccatcatc atgacgattt tccgtgcccg caaatcggct tcggtcgacg
     8581 acgcgaatct actcaaaggc tgagaacgcc acgatgacac acttcacctg gctgctcgtg
     8641 gcactgccac tggcgggcgc ggcaatcctg ctgttcggcg gcaggcgcac cgacgcgtgg
     8701 ggccacctgc tgggctgcgc cgcggcgctt gcggcattcg cggtgggcgc gaccctgctc
     8761 gccgacatgc tgggccgcag cgggaccgat cgcgtcatcc atcagaaggt cttcacctgg
     8821 atccccgtcg gccagctgca ggtcgacctc gggctgcaga tcgaccagct gtccatgtgt
     8881 ttcgtgctgc tgatcagcgg agtcggatcg ctgatccaca tttattcggt cggctatatg
     8941 gccgacgacc cggaccgccg ccgctttttc ggctatctca atctgtttct ggcctcgatg
     9001 ctgctgctgg tggtcgccga caactacgtg gtgctctacg tcggctggga aggcgtcggc
     9061 ctggcctcct acctgctgat cggtttctgg tatcacaagc cgtcggcggc cacggcggcc
     9121 aagaaggcgt tcgtgatgaa ccgggtcggt gacgcggggc tggcactggg aatgttcctg
     9181 atgttcagca ccttcggaac gctgtcgtac gcgggtgtgt tcggcgccgc gccggccgcc
     9241 ggcaagggcg cgctgaccgc gatgggattg ctgctgctgc tgggcgcctg cgccaagtcc
     9301 gcgcaggtgc cgctgcaggc gtggttgggt gacgcgatgg agggccccac cccggtgtcc
     9361 gcgctgatcc acgccgccac catggtgacc gccggcgtgt acctgatcgt gcggtccaat
     9421 cccctctaca acctgtcgcc cgacgcacaa ctcggcgtgg tcatcgtcgg cgcggtcacc
     9481 ctgctgttcg gggcgttcat cggctgcgcc aaggacgaca tcaaacgcgc cctggcggcc
     9541 tcgaccatga gccagatcgg atacatggtg ctggccgccg ggctgggccc ggccggctac
     9601 gcgtttgcga tcatgcatct gctcacccac ggcttcttca aggccggcct gttcctcgga
     9661 tcgggctcga tcatccatgc gatgcacgaa gagcaggaca tgcgccgcta cggcgggctg
     9721 cgcaaagcac tgcccatcac gttcgccacg ttcggtctcg gatacctggc gatcatcggt
     9781 gtgcctccgt tcgcgggatt cttctcgaag gacgccatta tcgaggcggc gctgggcgtt
     9841 ggcggggtcc ggggttacgt gctgggcggg gccgcgttgc tgggggccgg catcaccgcg
     9901 ttctacatga cccgcgtcat gctgatgacc ttcttcggtg acaagcgttg ggcaccggac
     9961 gcccatccgc acgaggcccc ggccgtgatg gcgtggccga tgatcctgct cgctgtcggg
    10021 tcggtgttct ccggcttcct gttcgcctac aacggcaccc tgcagcactg gctcgagccg
    10081 gtcgtcggcg ctcacgagga ggcggcccac gcccttcccg cctgggtcag caccgcgctg
    10141 gcgttgggtg tcgtcatcgt cggtatcgcg gtcgcctacc gcatgtatgg caccgcaccc
    10201 atcccgaggg tggctccggt gcgagtctcg gcgctcacca ccgccgcgcg cgccgacctt
    10261 tacggcgatg ccttcaacga ggaggtcttc atgcgccctg gcgcggaact gacccacgcg
    10321 ctggtcgaat tcgacgacga gggtgtggac ggctcggtca accggctggc cgcactggtc
    10381 ggccggactt cgaatcgcct gcgacgtctg caaaccggtt tcgcacgcaa ctacgcactg
    10441 tcgatgctgg cgggcgcggt gctgttgagc gcgctgatcc tggcggtgcg gctgtggtga
    10501 accgcgcctg cgacgatgca gtgggggcac gcacatgaac ggggctttcc tcaacggcgt
    10561 tccgtggctg agcgtgctgt ggctggtgcc gctggcgggt tcgctgctgg tcatcctgat
    10621 gccgccgggg cagcggcagc tcgccaagtg gaccggcctg ttcttcggcc tgctgacgct
    10681 ggcggtgtcc atcgtcatca ctctcggctt caaagccggg ggcgccacct accagtttgt
    10741 ggaaagccat cgatggatac cggcgttcgg cgccacgtac tccctcggcg tggacggcat
    10801 cgcggtggtg ctggtgctgc tgaccgcggt gctgatcccg ctgctacagg tggccggctg
    10861 gaacgacggc gtcgagcgca cccggggcgt gcaggcctac gtcgccctga cgctggccat
    10921 cgagtcgatg gtgctgatct cggtggtcgc gctgga
//
LOCUS       DASSGQ010000348        12395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_177507,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000348 DASSGQ010000000
VERSION     DASSGQ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12395
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177507"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VFI55_15440"
     CDS             <1..374
                     /locus_tag="VFI55_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HET7075456.1"
                     /translation="VPRPPYGQGAAAAAAGAQAMIDVSDGLIADLGHVAEASGVGIDL
                     STAALADDHDALTAAAAAVGADPWSWVLAGGEDHALVACFAGPAPAGWRVIGRVLDGP
                     ARVLVDGVQWSGYAGWESFGR"
     gene            384..674
                     /locus_tag="VFI55_15445"
     CDS             384..674
                     /locus_tag="VFI55_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HET7075457.1"
                     /translation="MTVYAIAQLRFTDRAAYDRYQARFMEVFRRHSGTLLAADESPQV
                     IEGRWDREKLVLMSFPDEAAFRGWAQSPEYQDISKDRRAGADTLVLLAKGLQ"
     gene            671..1354
                     /locus_tag="VFI55_15450"
     CDS             671..1354
                     /locus_tag="VFI55_15450"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET7075458.1"
                     /translation="MTARPLSDLVEPGWATALQPVADQVAQLGQFLRAEIAAGRRYLP
                     AGPNVLRAFTYPFDDVKVLIVGQDPYPTPGHAVGLSFSVAPEVRPVPRSLANIFDEYA
                     ADLGHPPPSCGDLTPWAQRGVLLLNRVLTVRPNNPASHRGKGWELVTECAIRALTARS
                     RPLVAILWGRDASTLKPILAEGNGVAIESPHPSPLSASRGFFGSRPFSRANDLLEGMG
                     VEPIDWRLP"
     gene            1360..2292
                     /locus_tag="VFI55_15455"
     CDS             1360..2292
                     /locus_tag="VFI55_15455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HET7075459.1"
                     /translation="MAALRAHRRGGPEVLVVERAPVPVPAADEVLVAVHAAGITFDEL
                     TWEETWTRDGVDRTPVIPSHEVSGVVAEIGSAVTGFKPGDEVYGLIGFDRDGAAADFV
                     AVPAADLAAKPSTVSHTRAAALPLAGLTALQALVDHAAVQPGEAVLVQGGAGGVGGLT
                     VQLAAILGARVTATVRSDAHELVSGFGAQRVIDVRAEALDETGRGFDVVVDTVGGATL
                     DKSFPVLRRGGRLVTLSAPPPPGKADEYGVTATFFIVTPNPDQLAELAALVDGRRLRV
                     EIAQTYPLDMGREAFESGRRPGRRAGKTVLVVRD"
     gene            complement(2328..2522)
                     /gene="rpmB"
                     /locus_tag="VFI55_15460"
     CDS             complement(2328..2522)
                     /gene="rpmB"
                     /locus_tag="VFI55_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009030042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET7075460.1"
                     /translation="MAAVCDICGKGPGFGKSVSHSHRRTSRRWDPNVQTVHIVNRPGG
                     NKRRINVCASCIKAGKVVRG"
     gene            2846..4570
                     /locus_tag="VFI55_15465"
     CDS             2846..4570
                     /locus_tag="VFI55_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET7075461.1"
                     /translation="MGHLGTSEGASNRLLDASALRDWAHTAVSDLITHIDEINRLNVF
                     PVADSDTGANMLFTMRSALAEAGVGTGAAAGPGCVARAAGALSAGALNGARGNSGVIL
                     SQILRGIADVTATAAADAGGELAHIDAAVLGAGLRCGVELVITSMGGDEVPGTIVSVL
                     RAAAEAVDECADEGLAAAITAAGDAAVVALEKTPEQLDVLADAGAVDAGGRGLLVLLD
                     ALRSTITGQAPPRTVYELSPRVPHPETLAEVPAPQFEVMYRVDGCDTPTAEALRNRLE
                     ELGESVAIAGAAAQDADTPDTYSVHVHTDDAGAAVEAGLQAGRLSRIVVSVLSSGAAG
                     MPAGSWTRERAVLALVDGDGAAELFAAEGACVLQSNPEPGGPATDISAHQLVRALVDT
                     GAAQVMVLPNGYVAAEELVAGCTAAIGWGIDVVPIPTGSMVQGLAALAVHESERHAVD
                     DGYTMARAAGVARHGSVRIAAESALTWAGPCRAGDGLGIAGDEVLIVAADAVEAAIGL
                     LDLLLASGGDLVTVLIGSGVECEALGMNATALGDILHRHVHDNHPGTELVTYRTGHRG
                     DLLLIGVE"
     gene            4573..6834
                     /gene="recG"
                     /locus_tag="VFI55_15470"
     CDS             4573..6834
                     /gene="recG"
                     /locus_tag="VFI55_15470"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET7075462.1"
                     /translation="MVALSDRLDYIVGAKAADSLDDVFGIRTVDDLLRHYPRSYTEGA
                     AVRGADGAGEERPEEGEHITIVDTITAAESFPMKKDRKRMCHRITVGSGRSKVTATFF
                     NANYIKKDLTKDTKVMLSGEVGFFKGVMQLTHPAFLILDPPDGRNRGTRSLKSIADAS
                     QAVSGEVLMEAFERRFFPIYPASTKLQSWDIFACVRQVLDVLDPVDDPLPAQLRAQLG
                     LISEDEALRAIHLAESEHERRRARERLTFDEAVGLQWALVARRHGELSESGPPAPPRP
                     DGLAAELSGQLPFELTAGQRDVLDVLSDGLASTRPLNRLMQGEVGSGKTIVAVLAMLQ
                     LVDAGYQCALLAPTEVLAAQHLRSINNVLGPLAMGGQLGGADLECGATRVALLTGSMT
                     AAQKKQVRAEIVSGQVGIVVGTHALLQDAVEFHNLGMVVVDEQHRFGVEQRDQLRAKA
                     RAGITPHLLVMTATPIPRTVALTVYGDLETLTLRELPRGRQPITTNVIFVNDKPGWLE
                     RAWQRIVEEVGGGRQAYVVAPRIDETDQPEKEVKKGAGEGERPPRPSATAEGLFARLS
                     SGELSELRLGLMHGRLPTDEKDAVMAAFRAGEIDVLVCTTVIEVGVDVPNATVMLVMD
                     ADRFGISQLHQLRGRIGRGEHPSLCLLASWVSSGSPAGRRLGAVADTMDGFVLADLDL
                     KERREGDVLGRNQSGRAVTLRLLSLADHGVFIEAARDFCVGAYAQDPSNAELALLAAR
                     FTNTDRIEYLDKS"
     gene            6890..7600
                     /locus_tag="VFI55_15475"
     CDS             6890..7600
                     /locus_tag="VFI55_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HET7075463.1"
                     /translation="MKGKLLLWLSALALLAIVVAYQTLGSLADERAEVAARAEIPTVQ
                     PGSDALAGVGVLPDRTRRNDYRRAVFGDAWTDDNDAPLGRNGCDTRDDILDRDLLDKT
                     FVSIKRCPNAVATGTLHDPYTNATIAFQRGAKVGESVQIDHIVPLAYAWDMGAYDWTF
                     PERLRFANDPANLLAVGGQANQDKGDSPPARWMPPNGAFACQYAIQFIAVLRGYQLPV
                     DQASVGVLRQAAATCPTG"
     gene            complement(7631..8476)
                     /locus_tag="VFI55_15480"
     CDS             complement(7631..8476)
                     /locus_tag="VFI55_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7075464.1"
                     /translation="MTGESGAAIPSIALNDENTMPVLGLGVADLSDDEAERAVSAALE
                     IGCRLIDTATGYGNEAGVGRAIAASGIPRAELFVTTKLATEDHGFSKAPVACSASLQR
                     LGLDYVDLYLIHWPAPAQGKYVDAFGGMIQSRGEGHVRSIGVSNFTEDHLTTVIDLTF
                     VTPAVNQIELHPLLNQAELRKADAQHNVVTQSYTPLVLGRLTDNPTVTSIAGEYGKTP
                     AQVLLRWNLQLGNAVIFRSAKAEHIASNLDVFDFELAAEHMDAINGLNDGTRLRPDPD
                     TYDGS"
     gene            complement(8483..9337)
                     /locus_tag="VFI55_15485"
     CDS             complement(8483..9337)
                     /locus_tag="VFI55_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7075465.1"
                     /translation="MGSSLAKNPQHLVGLNDGNSIPVVGLGVFKIPPTETEQAISAAL
                     RAGYRHIDTAAAYRNERETGRAIADSDVPRDQLYVVTKLANADQGYDSTLTAFDASMD
                     RLGLDYLDLYLIHWPLPALNRFVDTFKAFAHLHDQGRIRSIGVSNFEPEHLTVLIDAT
                     GIVPTVNQIELHPQFPQHDLREVHAQRGIATEAWAPLGRGALLTHPTVTAVAEGRGRT
                     PAQVLLRWHIQLGNIVIPKSVNPTRIASNFDVFDFELTADEMASISSLDDGTRLGPDP
                     RTFNFTGR"
     gene            9336..10454
                     /locus_tag="VFI55_15490"
     CDS             9336..10454
                     /locus_tag="VFI55_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7075466.1"
                     /translation="MTKSLPGASDLQGGAKQPPKGWTARVQSAVTGVGVKILPLIPAA
                     AKRAVLHGHAVIIDGNTFDPTLQLMLTGLHAVGIDGLVVDDDPAASRALLHELIVGLP
                     GPQIHVDVDHLSLPGPGGEIQARHYRPPGGVAAPLLVYYHGGGWVIGDLDVYDAFCRL
                     TCRDAGVHVLSIDYRLAPEHPAPAALEDAYAAFKWAYEHAGELGATPGRVAVGGDSAG
                     GNLAAVVCQQTRDEGGPAPILQWLIYPRTDFTAQSRSLSLFARGFLLTKRDMDWFQAQ
                     YLRGSGIDAGDPRLSPVFAESLSGLAPALIAVAGFDPLRDEGESYATALQAAGTAVDL
                     RYMGTVCHGFVNLFPLGGDSAVATNALISALRAHLTRA"
     gene            10557..11318
                     /locus_tag="VFI55_15495"
     CDS             10557..11318
                     /locus_tag="VFI55_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HET7075467.1"
                     /translation="MADKSKRPPRFDVKSTGKSGRLIQIGGTAIVVIFAVALVFYIVH
                     SHHKKGGATGAGDTVRVTSSKVVNQPGTSNPKAVVSFYEDFLCPACGNFERTFGPTVS
                     NLIDSGAIAAEYSMVSILDNAKNQNYSSRAGAAALCVADESLDAFRRFHTALFTTAVQ
                     PDERGSSFPDNAKLIELAREAGVVGTVPDCINSGKYLAKVTGEAAAAHINATPTIKIN
                     GDDYEPSTPDALVAKIKSIVGDVPGIDTAVAPAAS"
     gene            11360..11962
                     /locus_tag="VFI55_15500"
     CDS             11360..11962
                     /locus_tag="VFI55_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HET7075468.1"
                     /translation="MTPDSGSLTRVPAASAWFVLIAGVVGLVASITLTVEKIKILLNP
                     SYVPSCNINPIVSCGSVMVTPQASVLGFPNPLLGLVGFTVVIVTGVLAVGKVPLPQWY
                     WVGLTVGILIGCVLVHWLIFQSLYRIGALCPYCMVVWVVTVSLLVVVASITFRPALQR
                     SDSGIAWAVYRWRWSIAALWFTAVFLMIMVRFWDYWSTLL"
     gene            11973..>12395
                     /gene="rsmD"
                     /locus_tag="VFI55_15505"
     CDS             11973..>12395
                     /gene="rsmD"
                     /locus_tag="VFI55_15505"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET7075469.1"
                     /translation="MTRIIGGIAGGRRIAVPPKGTRPTTDRVRESLFNIVAARLDLTG
                     LAVLDLYAGSGALGLEALSRGAASALFVESDQRTAAVIARNIETLGLRGATVCRGAVA
                     TVLAAGTASPVDLVLADPPYDVEAAEVDAVLATLTARGW"
BASE COUNT         1938 a         4015 c         4384 g         2058 t
ORIGIN      
        1 tggtgccgcg gccgccctat ggccagggag cggcggccgc ggcggcgggc gcgcaggcga
       61 tgatcgacgt ctcggatggc ctcattgccg acctggggca cgtcgccgag gcgtccggcg
      121 tgggcatcga tctgtccacc gcggccctgg ccgatgacca tgacgcgctg accgcggccg
      181 ccgccgccgt cggcgccgat ccctggtcct gggtgctggc cggcggcgaa gaccacgcgc
      241 tggtggcctg tttcgccggc cccgcaccgg ccgggtggcg ggtcatcggg cgggtgctgg
      301 acgggccggc ccgcgtgctt gtcgacggcg tccagtggag tggttacgcc ggctgggagt
      361 cgttcgggcg ttagggtggc gcggtgaccg tctacgcgat cgcccagctg agattcaccg
      421 accgggcagc ctatgaccgg taccaggcga gattcatgga agtcttccgc cgtcactcgg
      481 gaaccctgct cgccgccgat gagtcgcccc aggtcatcga ggggcggtgg gaccgtgaga
      541 agttggtgct gatgtcgttt ccggacgagg ccgcgttccg cggatgggcg caatcacccg
      601 aataccagga catctcgaag gatcggcgag cgggtgccga cacactggtg ctgctggcca
      661 aagggctgca atgaccgcgc gcccgttgag tgacctcgtc gaaccgggct gggcgaccgc
      721 gctgcagccg gtggccgacc aggtcgccca gctgggccaa ttcctacggg ccgagatcgc
      781 ggccgggcgc aggtacctac ccgccggccc gaatgtgttg cgcgccttca cttatccgtt
      841 cgacgacgtc aaggtcctga tcgtcgggca ggatccgtac ccaacgcccg gacacgctgt
      901 gggcctcagc ttttcggtgg ctcccgaggt gcgtccggtg ccgcgcagcc tggccaacat
      961 cttcgacgag tacgccgccg acctgggcca tccaccgccc tcgtgcggtg atttgacgcc
     1021 ctgggcgcaa cgcggtgtgc tgctgctgaa cagggtgctg accgtgcgcc ccaacaaccc
     1081 ggcgtcacat cggggcaagg gctgggaact ggtgaccgag tgtgcgatcc gcgcgctcac
     1141 cgcgcggtcg cggcccctgg tggcgatcct gtggggccgc gacgcgtcaa cgttgaaacc
     1201 cattctggcc gaaggcaacg gtgtggccat cgagtcgccg cacccgtcgc cgctgtcggc
     1261 gtcgcgcggg ttcttcggct cgcgtccgtt cagccgcgcc aacgacttgc tggagggaat
     1321 gggcgtcgag ccgatcgatt ggcggctgcc ttgacggaga tggctgcgct gcgggcacac
     1381 cgccggggcg gcccggaggt actggtcgtc gagcgcgcac ccgtgccggt cccggccgcc
     1441 gacgaggtct tggtcgcggt acacgccgcg gggatcacgt tcgacgagtt gacgtgggag
     1501 gaaacgtgga cccgcgacgg agtcgaccgg actccggtca tcccgtcgca tgaggtatcc
     1561 ggcgtggttg ctgaaatcgg ttcggcggta acgggtttca agcccggcga cgaggtgtac
     1621 ggactcatcg gattcgaccg cgacggcgct gcggccgatt tcgttgcggt gccggcggcc
     1681 gacctggccg ccaagccgtc gacggtgtcg catacccgcg cggccgcgct gccgctggcc
     1741 ggactgaccg ccctgcaagc cctcgtcgac cacgccgccg tccaacccgg agaagcggtg
     1801 ctggtgcagg gcggggccgg tggagtcggt ggcttgaccg tccagctggc cgccatcctc
     1861 ggcgcacggg tcaccgccac cgtgcgcagc gatgcccacg agctggtgag cggcttcggt
     1921 gcgcagcggg tgatcgatgt gcgcgccgag gcgttggacg agaccggccg cgggttcgac
     1981 gtggtcgtcg acaccgtcgg cggcgcgacg ttggacaagt cctttcccgt gctgcgccgg
     2041 ggcggacggc tggtcacctt atcggcgccc cctccgccgg ggaaggccga cgaatacggg
     2101 gtcacggcaa catttttcat cgtcacaccc aatccagacc agctcgcgga actggctgcg
     2161 ctggtggatg gccgccgact gcgcgtggaa atcgcccaga cgtatcccct cgacatgggg
     2221 cgcgaagcat tcgaaagtgg tcggcgcccg ggccggcgcg ccggaaagac cgtcctcgtc
     2281 gtccgggact gaccagaacc ggctgagggc ggcgacccgc aggcccgcta gccgcggacg
     2341 accttgccgg ccttgatgca ggacgcgcag acgttgatgc gccgcttgtt gccgccgggg
     2401 cggttcacga tgtgcacagt ctgcacgttg gggtcccacc ggcggctggt gcggcggtgg
     2461 gagtgcgaca ccgacttgcc gaagccgggg cctttcccgc agatatcgca cacagcggcc
     2521 atcgttcaaa ctcctcaaat cttctcaggg tccggcacgc aggaaagatc tggacgagcc
     2581 gcgacagccc gggattccgg gccaaaccag gataccgact gcgtgggaaa ccaccaaaac
     2641 gatcaacagc cgccgcacgg ctgacggcgg gtgttgtcgc cccctctggc taggctcagt
     2701 gggcgggttc accgaggcca ggcgggtgct gcggcacggc tggcggccgc gcgtgtcgcc
     2761 gcggggctac gctggcgccc accgggaggc cgacactggc aggcacagga aggcgctggg
     2821 aggcgctggg aggctcggag ggaggttggg tcacttgggc acgtcggagg gcgcgtcgaa
     2881 tcgtctgctg gacgcatccg ccctgcggga ctgggcgcac accgccgtca gtgacctgat
     2941 cacccacatc gacgagatca accggctcaa cgtattcccc gtcgcggatt ccgataccgg
     3001 cgccaacatg ctgttcacca tgcgttcggc gctggccgag gcgggcgtgg ggaccggcgc
     3061 cgctgccggc ccggggtgtg tggcccgcgc cgcgggagcc ctgtcggcgg gcgccctgaa
     3121 cggtgcgcgc ggcaattccg gcgtgatcct gtcgcagatt ctgcgcggga tagccgacgt
     3181 caccgcgacc gcggccgccg atgccggcgg cgagctggcc catatcgacg ccgccgtgct
     3241 cggggccgga ttgcggtgcg gcgtcgagtt ggtcatcacg tcgatgggcg gcgacgaagt
     3301 tcccggaacc atcgtctcag tgctgcgggc cgccgccgaa gctgtcgacg agtgcgccga
     3361 cgaggggctg gccgcggcga tcaccgccgc cggtgacgcg gcggtggtgg cactggaaaa
     3421 gacccccgag cagctcgacg tgctcgccga cgccggggcg gtggacgccg gcgggcgcgg
     3481 cctgctggtc ctgctggacg cgctgcggtc caccatcacc gggcaggcgc cccctcgcac
     3541 ggtgtacgag ctgtcgccgc gcgtgccgca ccccgaaacc ctcgccgaag tccccgcccc
     3601 gcagttcgag gtgatgtacc gggttgacgg ttgcgatacg ccgacggccg aggcgctgcg
     3661 gaaccggctc gaggagctcg gcgaatcggt ggccatcgct ggggcggccg cgcaggacgc
     3721 cgacacgccc gacacctact ccgtccacgt gcacaccgac gacgccgggg ccgcggtgga
     3781 agcgggactg caggccggcc ggctgagccg gatcgtggtc tcggtgctga gctcgggcgc
     3841 cgccgggatg ccggcgggta gctggacgcg ggagcgggcg gtgctggccc tcgtcgacgg
     3901 cgacggtgcc gccgagctgt tcgccgccga gggcgcctgc gtgctgcagt cgaatcccga
     3961 gccgggcgga ccggccaccg atatcagcgc gcaccagctg gtgcgggcgc tggtcgacac
     4021 cggcgccgcg caggtgatgg tgctgcccaa cggctatgtg gcggcggagg agctggtggc
     4081 cggctgcacc gcggccatcg gctgggggat cgacgtggtg cccataccga cggggtcgat
     4141 ggtgcagggg ttggccgcgc tggccgtgca cgaatcggaa cgacacgcgg tcgacgacgg
     4201 atacaccatg gcccgcgcgg cgggcgtggc ccggcacgga tcggtgcgca tcgccgccga
     4261 gagcgccttg acctgggccg ggccgtgccg cgcgggcgac ggcctgggca tcgcgggcga
     4321 cgaggtgctg atcgtggccg ccgacgcggt cgaggcggcg atcgggctgc tggatctgct
     4381 gctggcgtca ggcggcgacc tggtgacggt gctgatcggt tccggcgtcg agtgcgaagc
     4441 cttgggtatg aatgcgaccg cgctcggcga catcctgcac cggcatgtgc acgacaatca
     4501 tccgggaacc gagctggtca cctaccgcac cggccaccgc ggggacctgc tgttgatcgg
     4561 ggtcgagtag cggtggtggc gctgtccgat cggctggact acatcgtggg cgccaaggcg
     4621 gcggattccc tcgacgacgt gttcggcatc cggaccgtcg acgacctgct gcgccactat
     4681 ccgcgcagtt acaccgaagg cgcggccgtg cggggcgccg acggcgccgg cgaggagcgg
     4741 ccggaggagg gggagcacat caccatcgtc gacacgatca ccgctgcgga gtcgtttccg
     4801 atgaagaagg accgcaaaag gatgtgtcac cgcatcaccg tcggatccgg acggagcaag
     4861 gtgaccgcca cgttcttcaa cgcgaattac atcaagaagg acctcaccaa agacaccaag
     4921 gtgatgctgt ccggggaagt cgggttcttc aaaggggtga tgcagctcac gcatcccgca
     4981 ttcctcattc tcgacccgcc ggacggacgc aaccgtggca caagatcgct gaaaagcatt
     5041 gccgacgcgt cgcaagcggt cagcggcgag gtgctgatgg aggcgttcga acgtcgcttc
     5101 ttcccgatct atccggccag caccaagctg cagagctggg acatcttcgc ctgtgtgcgc
     5161 caggtgcttg acgtgctgga tccggtggac gatccgctgc ccgcccaact gcgggcgcag
     5221 ctcgggctga tctccgaaga cgaagcgctg cgcgccatcc atctcgccga gagcgagcac
     5281 gaacgccggc gtgcgcgtga gcggttgacc ttcgacgaag ccgtcggcct gcagtgggcg
     5341 ctggtggccc gccgccacgg tgagctctcg gaatcagggc ctcccgcacc gccgcggccc
     5401 gacggcctgg cggccgaact gtcggggcag ttgccgttcg agctgacggc cggccaacgc
     5461 gacgtcctcg atgtgctgtc ggacggcctg gcgtcgacgc gtccgttgaa ccgcctgatg
     5521 caaggcgaag tcggctcggg caagacgatc gtcgcggtgc tggcgatgct gcaactggtc
     5581 gatgccggct accagtgtgc gctgctggcg ccgacggaag tgcttgccgc acaacatctt
     5641 cgatcgatca acaacgtgct gggtccgttg gcgatggggg gtcagctggg tggggcggac
     5701 ctggagtgtg gggccacccg ggtggcgctg ctcaccgggt cgatgacggc cgcgcagaaa
     5761 aagcaggtcc gcgccgagat cgtcagcggt caggtcggca tcgtcgtcgg cacccacgcg
     5821 ctgctgcagg acgcggtgga gttccacaac ttgggcatgg tggtggtcga cgaacaacac
     5881 cggttcggcg tcgagcagcg agatcagttg cgcgccaagg cccgtgccgg aatcacacca
     5941 cacctgctgg tgatgacggc gacgccgata ccgcgcacgg tcgcgctgac cgtgtacggc
     6001 gacctggaga ccctgacgct gcgtgaactc ccgcgcggac gccaaccgat caccaccaac
     6061 gtcatcttcg tcaacgacaa gcccggctgg ctcgagcgtg cctggcagcg gattgtcgaa
     6121 gaggtcggcg gcgggcgtca ggcctacgtg gtcgcgccgc gcatcgacga gaccgatcag
     6181 ccggagaaag aagtcaaaaa gggggcggga gaaggggagc ggcccccccg accgtcggca
     6241 accgccgagg gcctgttcgc gcgactgtct tccggcgagc tgtcggagct gcggctgggg
     6301 ctcatgcacg ggcggttgcc caccgacgag aaggacgccg tgatggccgc gttccgcgcg
     6361 ggcgaaatcg acgtgctggt ctgcaccacc gtcatcgagg tgggcgtcga cgtccccaat
     6421 gccacggtca tgttggtgat ggacgccgac cggttcggaa tcagccagtt gcatcagctg
     6481 cggggccgca tcggccgtgg cgaacatccg agcctgtgtc tgctggccag ctgggtgtcg
     6541 tcgggctcgc ctgccggtcg gcggctgggt gccgtcgccg acacgatgga cggcttcgtc
     6601 cttgccgacc tggacctcaa ggagcgccgg gagggagacg tgctgggccg caaccagtcc
     6661 ggcagggcgg tcaccctgcg gctgttgtcg ctggccgacc acggcgtgtt catcgaagcc
     6721 gcccgcgact tctgcgtggg ggcctacgcc caggacccgt cgaatgccga attggctttg
     6781 ctggcagcgc gattcaccaa taccgatcga atcgaatacc tggacaagtc atgaccgcca
     6841 gcgacgatgc agtgggggca cctcccgctt gcgggggaga gcggcgctgg tgaagggcaa
     6901 gctgctgctg tggttgtcgg cgctcgcgct gctcgcgata gtggtcgcct atcagacgct
     6961 cggatcgttg gcggacgagc gggcagaagt cgccgcgcgc gccgaaattc ccacggtgca
     7021 acccggtagc gacgcgctgg cgggcgttgg agtgctgccg gaccggaccc gccgaaacga
     7081 ctaccgcagg gcggtgttcg gcgatgcctg gaccgacgac aacgacgctc cgctcgggcg
     7141 caacggatgt gacacccgcg acgacattct ggatcgggac ctcctcgaca agacatttgt
     7201 gtcgatcaag cgctgcccga acgcggtggc gaccggcacc ctgcacgacc cgtacaccaa
     7261 cgccaccatc gcttttcagc gcggtgcgaa ggtcggcgaa tcggtgcaga tcgaccacat
     7321 cgtcccgctc gcctacgcgt gggacatggg ggcctacgac tggacgttcc ccgagcggct
     7381 ccggttcgcc aatgatccgg ccaatctgct ggccgtcgga ggccaggcca atcaggacaa
     7441 gggcgattcg ccgcccgcgc ggtggatgcc gcccaacggc gcgttcgcgt gtcagtacgc
     7501 catccagttc atcgcggtgc tgcgcggtta ccagttgccg gtggaccagg cttcggtggg
     7561 tgtgctgcgt caggccgcgg ccacctgccc gacggggtag cgcgcagggg ctaggaaccc
     7621 ttaggaaccc ttaggagccg tcataggtgt ccggatccgg gcgcaaccgg gtcccgtcgt
     7681 tgagaccgtt gatcgcatcc atgtgctcgg cggccaattc gaaatcgaag acatccaggt
     7741 tgctggcaat gtgctcggct ttggccgacc ggaagatcac cgcattaccc aattgcaggt
     7801 tccaccgcag cagtacctgt gcgggggtct tgccgtattc accggcgatc gaggtcaccg
     7861 tcgggttgtc cgtcaacctg ccgagaacca gcggggtgta ggactgggtg acgacgttgt
     7921 gctgcgcatc ggctttgcgc agctcagctt ggttgagcag cgggtgcagc tcgatctggt
     7981 tgaccgccgg ggtgacaaag gtgaggtcga tgaccgtggt caggtgatcc tcggtgaaat
     8041 tggagacgcc gatcgagcgg acatggcctt ccccgcggga ctggatcatg ccgccgaagg
     8101 catcgacgta cttgccctgc gccggggccg gccagtgaat caggtagagg tcgacgtaat
     8161 cgaggccaag tcgctgcaag ctcgcgctgc aggccaccgg ggccttgctg aaaccgtggt
     8221 cttcggtggc cagcttggtg gtgacgaaca gctctgcgcg gggaatcccg gatgccgcga
     8281 tcgcacggcc gacgccggct tcgttgccgt accccgtcgc ggtgtcgatc agccggcagc
     8341 cgatttccag cgcagccgac acggcgcgtt cagcctcgtc gtcagacaaa tcggcgacgc
     8401 ccaggccaag caccggcatg gtgttctcgt cattgagagc tattgagggt atggcggcgc
     8461 ccgactcgcc agtcaacgtc attcacctac ctgtgaaatt gaaggttcgt ggatctggac
     8521 ccaaccgggt gccatcgtcc agcgaggaga tcgacgccat ttcatcggca gtgagttcga
     8581 aatcgaacac gtcgaagttg ctcgcaatcc gtgtggggtt taccgacttg gggatcacga
     8641 tattacccag ctggatgtgc catcggagca acacctgagc gggtgtccgc ccgcgtcctt
     8701 cggcgacggc cgtgacagtc ggatgagtca acagcgctcc gcggcccagc ggcgcccacg
     8761 cctccgtcgc gatgccgcgt tgggcatgca cttcgcgcaa gtcgtgttgg ggaaactgtg
     8821 gatgcagctc gatctggttg accgtcggaa cgatgccggt ggcatcgatg aggactgtga
     8881 ggtgctcggg ctcgaagttg ctgactccga tggagcggat gcggccctgg tcgtgcaggt
     8941 gggcgaaagc cttgaaggtg tcgacgaacc tgttgagcgc cggcagcggc cagtggatca
     9001 ggtacaggtc gagatagtcg agcccgaggc ggtccatgct cgcgtcgaat gccgtcagcg
     9061 tgctgtcgta accctggtcg gcattcgcca atttggtcac cacgtagagt tggtcgcgcg
     9121 gcacgtcgga atcggcaatc gcccgcccgg tctctcgttc atttcggtag gccgcggcgg
     9181 tgtcgatatg ccggtagccg gcgcgcagcg cggcactgat cgcctgctcg gtctccgtcg
     9241 gagggatctt gaacactccg agcccaacga cggggatcga attgccgtca ttcagcccga
     9301 caaggtgttg agggtttttc gcgagggagc tgcccatgac caagagtctg ccaggcgcgt
     9361 cggacctcca gggtggagca aaacagccgc cgaaggggtg gaccgctcgc gtgcagagcg
     9421 cggtcaccgg cgtcggtgtc aagatcctcc cgttgatccc ggccgccgca aagcgggccg
     9481 tgttgcacgg tcacgcggtc atcatcgacg gcaacacgtt cgaccccacg ctgcagctga
     9541 tgttgacggg tttgcacgcc gtcggtatcg acggcttggt cgtcgacgac gatccggccg
     9601 catcccgtgc cctgttgcac gagctgattg tcgggcttcc cgggccgcag attcacgttg
     9661 acgtggacca cctgtcgcta cccggcccgg gcggcgagat ccaggcgcgg cactaccgcc
     9721 cgcccggtgg ggtggccgct ccgctgctgg tttactacca cggcggtggc tgggtgatcg
     9781 gcgatctgga cgtctacgac gcgttctgcc ggctgacctg ccgtgacgcc ggcgtccatg
     9841 tgttgtcgat cgactaccgg ctggctcccg aacacccggc ccctgccgcg ctggaggatg
     9901 cctatgcggc cttcaagtgg gcgtatgaac atgccggtga actcggcgcg acccccggac
     9961 gggttgcggt cggcggtgac agtgcgggtg gcaacctggc ggccgtcgtg tgccaacaaa
    10021 cgcgcgacga agggggaccc gcccccatcc tgcagtggct gatctaccca cgaaccgact
    10081 tcaccgcgca aagccggtcg ctcagcctgt tcgcccgcgg cttcttgctg accaagcgtg
    10141 acatggactg gttccaagcg cagtacctga ggggttcggg tatcgacgcg ggagacccgc
    10201 ggctgtcgcc ggtgttcgcc gagtcactgt ccgggctggc gccggcgctg atcgcggtcg
    10261 cgggattcga cccgttgcgc gacgaaggcg agagctacgc gaccgcgctg caagccgccg
    10321 gaactgcggt ggacctgcgg tacatgggta cggtgtgcca cggtttcgtc aacctgtttc
    10381 cgctgggcgg cgatagcgcc gtcgcgacca acgcattgat ttcggctctg cgcgctcacc
    10441 tgacccgggc ctgatctgcc ggaccgttct cgccggtaat ctagaggcgc cgttgtccga
    10501 ccgacggacg aacccgcacg cccgaccaat cgagcaccga aggatcagcg aaacctgtgg
    10561 ccgacaaatc caaacgtccc ccgcgattcg acgtgaagtc caccggcaaa tccggccggc
    10621 tcatccagat cggcggcacc gcgatcgtcg tgatcttcgc ggtcgcgctc gtcttctaca
    10681 tcgtccattc gcaccacaag aagggcggcg ccaccggcgc cggagatacg gtgcgggtga
    10741 cgtcgagcaa ggtggtcaac cagccgggga ccagcaaccc caaggccgtg gtctcgttct
    10801 acgaggactt cctgtgcccg gcctgcggta atttcgagcg cactttcggt ccgacggtgt
    10861 ccaacctcat cgattccggc gccattgcgg ccgaatactc catggtgtcg atcctcgaca
    10921 acgcgaaaaa ccagaactat tcctcgcggg cgggcgcggc ggccttgtgt gtcgccgacg
    10981 agtcccttga cgcgtttcgc cggttccata ccgcgctctt caccaccgcc gtccagccgg
    11041 acgaacgggg tagcagcttc ccggacaacg cgaagctgat cgaactcgcc cgcgaagccg
    11101 gcgtcgtggg cacggtcccg gactgcatca acagcggcaa ataccttgcc aaggtcaccg
    11161 gtgaggccgc ggccgcacac atcaacgcaa cgccgaccat caagatcaac ggcgacgatt
    11221 acgagccgtc gacgccggat gcgctggtcg ccaagatcaa gagcattgtg ggcgacgtcc
    11281 cgggcatcga caccgcggtc gcgcccgctg cgtcgtgacc accccggcgt cagcacaacc
    11341 cgccgagcac gccggcgacc tgacaccgga ttcgggttcc ctaacgcgcg tgcccgcggc
    11401 aagcgcgtgg tttgtgctga tcgccggcgt ggtcggcttg gtggcatcga tcacgctgac
    11461 ggtggagaag atcaaaattc tgctcaaccc gtcctacgtg ccgtcgtgca acatcaaccc
    11521 gatcgtgtcg tgcggctcgg tgatggtgac cccgcaggcc tcggttctgg gattccccaa
    11581 cccgctgctc ggtctggtgg gcttcaccgt ggtgatcgtc acgggtgtgc tggccgtcgg
    11641 gaaagtcccg ttgccgcaat ggtattgggt cggattgact gtcgggatcc tgatcggctg
    11701 tgtgttggtg cactggctga tctttcagag cctgtaccgc atcggggcgt tgtgcccgta
    11761 ttgcatggtg gtctgggtgg tcaccgtctc gttgttggtg gtcgtcgcgt cgatcacgtt
    11821 tcgaccggcg ctgcaacgct ccgacagtgg catagcgtgg gcggtttacc gatggcgatg
    11881 gtcgatcgcc gcactctggt tcacggcggt gtttctgatg atcatggtgc ggttctggga
    11941 ctactggtcg acactgctgt gagcgtccgc gaatgacgcg gatcatcggc ggtatcgccg
    12001 ggggccgacg aatcgccgtc ccgccgaagg gaaccaggcc gaccaccgat cgagtacggg
    12061 aatcgctctt caacatcgtg gccgcgcgcc tggacctgac cggtctggcg gtgctggacc
    12121 tatacgcggg ttcgggcgca ctcgggctcg aggcgctgtc gcggggtgcg gcctccgcgc
    12181 ttttcgtgga gtcagatcag cgcaccgcag ccgtcatcgc gcgcaacatc gagaccctgg
    12241 gcctgcgcgg tgcgacggtg tgccggggcg cggtggccac ggtcttggcg gccggcaccg
    12301 cgtcgccggt ggacctggtg ttggccgacc cgccctacga cgtcgaggcg gccgaggtgg
    12361 acgcggtcct ggccaccttg actgcccgcg gctgg
//
LOCUS       DASSGQ010000349         2758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_755262,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000349 DASSGQ010000000
VERSION     DASSGQ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755262"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="VFI55_15510"
     CDS             <1..145
                     /locus_tag="VFI55_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005198505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET7075470.1"
                     /translation="FNGDGAPGQLWVFWLAPLVGGLIGGLIYPLLFERGKAAHAEAPR
                     AAK"
     gene            complement(199..924)
                     /locus_tag="VFI55_15515"
     CDS             complement(199..924)
                     /locus_tag="VFI55_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019927405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7075471.1"
                     /translation="MSGQTIALVTGANKGIGYEIAAGLGALGFSVGVGARDPERREAA
                     VHKLRTGGADAFGVPLDVTDDASVAAAARLVEEYAGRLDVLVNNAGVAGGMPQAPTGV
                     DLADMRNALETNVFGVIRVTNAMLPLLRRSSSPRIVIVSSKVGSLSLQAAPAAETGPI
                     SAVYAPSKTFLNAVTVQYAKELRDTNILINAACPGHCATDLTGFQAPRTPEQGAAIAI
                     RLATLPDDGPTGGFFDDAGVVPW"
     gene            1040..1918
                     /locus_tag="VFI55_15520"
     CDS             1040..1918
                     /locus_tag="VFI55_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7075472.1"
                     /translation="METRELRYFVAVAEELHFGRAAQRLGIAQPPLSRAIQQLERRLG
                     APLLERSHRGIALTEAGLVLLREGRVALEAVDAADRRTRRAAAAVTGQAGVVLVVKAG
                     ASSELLSKLLDAYAAEPDAVPVDVILCGVGEQERLLRDGRADVALLHRPFDSTAGFDT
                     EELYTEGQVAVLPAGHPLTAHTHLQMADITALPNLPMPRWPRPDGSYSDGPGPLVRDL
                     TQLCQLIALGRACAVLPESFRAQLGDDLAAVPVPDAPMVTTVIAWPPHTRSRAVARLV
                     QVATRLGGAIPIQRLA"
     gene            2036..2581
                     /locus_tag="VFI55_15525"
     CDS             2036..2581
                     /locus_tag="VFI55_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HET7075473.1"
                     /translation="MLLLVCLSMFVLATLMSLIGQVSGTPYITGGDSPAGTDCSGLAS
                     WVANAATDRPVFGDRFNTGNEEAALLARGFQYGTAPNALVIGWNGGHTAVTLPDGTPV
                     SSGERGGVHIGGDGAYQRGFTHHMFLPMPPDGDGPGLPPPPDAPPPPDAPAVLVDATA
                     PAIGAPAPDLPSAGDPGIANP"
BASE COUNT          411 a          921 c          939 g          487 t
ORIGIN      
        1 cttcaacggg gacggtgccc ccggtcaact gtgggtgttc tggctggcac cactggtcgg
       61 gggcctcatc ggcggtctca tctacccgct gctgttcgag cgcgggaagg cggcgcacgc
      121 cgaagcgccg cgagcggcca aatgaaggcc gacgcggcgc agtagcggtt caaatcagcg
      181 gcgccgccgg gcccggtgtc accagggcac caccccggcg tcgtcgaaaa acccgccggt
      241 cggtccgtcg tcgggcagtg tggcgagccg gatcgcgatc gccgcaccct gttcgggggt
      301 gcggggggct tggaagccgg tgagatcggt cgcgcagtgg ccggggcagg cggcgttgat
      361 caggatgttg gtgtcgcgca actccttggc gtactggacg gtgaccgcgt tgaggaaggt
      421 cttcgacggc gcgtacacgg cggaaatcgg gccggtctcg gcggccgggg cggcctgcag
      481 tgacagcgag ccgaccttgc tggacacgat cacgatccgc ggcgacgacg aacggcgcag
      541 cagcggcagc atcgcgttgg tgacacggat tacgccgaac acgttggtct cgagcgcgtt
      601 ccgcatgtct gcaagatcaa ccccggtggg agcctgcggc atcccgccgg ccaccccggc
      661 gttgttgacc agcacgtcga ggcggcccgc atattcctcg accagcctgg cggcggcggc
      721 cacgctcgca tcgtcggtca cgtcgagtgg cacgccgaac gcgtcggcgc cgccggtgcg
      781 cagcttgtgc acggcggcct cccggcgctc cgggtcgcgg gcgccgacgc cgacgctgaa
      841 gcccagggcg cccaggcccg ccgcgatctc gtacccgatt cctttgttcg cgccggtgac
      901 cagcgcgatc gtctgtccgc tcatgtagtt catgctggcg cgcgctcggg tgcgtctgcc
      961 aacaccgtat gggtgggcgg cgataccttg ccggtatcga tccatgtcag ggcgaggccg
     1021 tgaccggtac cgtggcggca tggagacgcg ggagctgcgg tacttcgttg ccgtcgccga
     1081 ggagctgcac ttcgggcggg cggctcagcg gctcgggatc gcgcagcccc cgctgtcgcg
     1141 ggcgatccag cagctcgaac ggcggctcgg ggcgccgctg ctggaacgct cccaccgcgg
     1201 aatagccctg accgaggccg ggctggtgct cctgcgcgag ggcagggtgg ccctcgaagc
     1261 ggtcgacgcc gccgaccgcc gcactcgccg cgcggccgcg gcagtgaccg gccaggccgg
     1321 ggtggtgctc gtcgtcaagg ccggagcgtc gagcgaactg ctgtcgaaac tgctcgacgc
     1381 ctatgccgcg gaacccgacg cggtccccgt cgatgtgatc ctgtgcggag tcggcgagca
     1441 ggagcggctg ctgcgcgacg ggcgggccga cgtggcgctg ctacaccgcc cgttcgactc
     1501 caccgccggg ttcgacaccg aggagctcta caccgaggga caggtcgcgg ttctgccggc
     1561 cgggcacccc ctcaccgctc atacccacct gcagatggcc gacatcaccg cgctgccgaa
     1621 cctgcccatg ccccgctggc cgcgccccga tggcagctac tccgacggcc ccggcccgct
     1681 ggttcgagac ctgacgcagc tgtgccagct gatcgcgctc ggccgtgctt gcgcggtcct
     1741 gccggagtcg ttccgcgctc agttgggcga cgaccttgcc gcagtgccag tgcccgatgc
     1801 accgatggtg acgaccgtga tcgcctggcc gccgcacacc cggtcccgag cggtcgcacg
     1861 cctcgtccaa gtcgcgaccc gcctcggtgg agccatcccg attcagcgcc tcgcttaaat
     1921 gctcaacagt tttgtgtcga taaatatctc gcgttgtaat atgtttgctg tagatatagc
     1981 caacaccttt atttaattgc acatgatgcg cgccacaata tttggttatg gctgaatgtt
     2041 gttgctagtg tgcctttcta tgtttgttct agcaacgttg atgtcactca tcggccaggt
     2101 ttccgggacg ccctatatca ctggcggaga ttctcccgcc ggaaccgatt gttcagggct
     2161 tgcctcatgg gttgccaacg cggcaactga ccggcctgtt ttcggggacc gattcaacac
     2221 cgggaatgag gaagccgctt tgctggcgcg gggctttcaa tatggaaccg ctcctaacgc
     2281 cttggtgatc gggtggaacg gtggccacac tgcggtgaca ctgccggacg gaaccccggt
     2341 ttccagcggt gagcgcggtg gcgtgcacat cggtggtgac ggcgcttacc agcgtggatt
     2401 cacgcaccac atgttcctgc cgatgccgcc ggacggtgac ggcccgggcc tgcccccgcc
     2461 gccggacgcg ccgccgccgc cggacgcgcc ggccgttctg gtcgacgcca cggccccggc
     2521 aataggggcg ccggcgcctg atttgccgtc ggccggcgac cccggaattg cgaatccata
     2581 acaattaaat cgcgtgcgtg gagcctccgg cagatcttgc cgagcttgta tcccacccgc
     2641 cggtgctgag tttctgaacc gattcacaat tcgtggattg tgaactggca cggatctgaa
     2701 tcggattgtg tggtgcgtca catatcttcc cctctgggca ccgcacgtcg attcggag
//
LOCUS       DASSGQ010000350         3836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_512950,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000350 DASSGQ010000000
VERSION     DASSGQ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512950"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(334..777)
                     /locus_tag="VFI55_15530"
     CDS             complement(334..777)
                     /locus_tag="VFI55_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7075474.1"
                     /translation="MSAYRTVVVGTDGSDSSMRAVDRAAAIAGADAKLFIASAYLPQH
                     EDTRAADALRDESYKVSGTAPIYAILQDAKERAHAAGAKNVEERSIVGAPVDALVKLA
                     EDEKADLLVVGNVGLSTIAGRLLGSVPANVSRRAKVDVLIVHTTN"
     gene            1100..2674
                     /locus_tag="VFI55_15535"
     CDS             1100..2674
                     /locus_tag="VFI55_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075475.1"
                     /translation="MSMPTLEQRAADVVEQPAPALARRGSLDVWAIATVAAVISGAWA
                     SRPSLWFDEAATITASASRTVPELWKLIGHIDAVHGLYYFLMHGWFALFPPTEFWSRV
                     PSALAVGAAAAGVTVFTKRFVPGRATGACAGVIFAILPRTTWAGIEARSYALAVAAVI
                     WLTVLLIAAVRTNRPRLWLFYALALMLSVLLSLNLLLLVPVYAVMVPLLARTKSPVIR
                     WAVSSAVAVGAMTPFIVFAHGQVGQVNWIYPVSWHYAFDIILRQYFDHSVPFAVLTAV
                     LIVAAAVARLKGARGPAGDVRTLLTVCAAWIVIPTGLIVAYSAISEPVYYPRYLILTT
                     PAMAVIIAVCIVTIARKPRPIAAVLLLFALAGLPDYLFTQRWPYAKEGWDYSQVADLI
                     SSHAAPGDCLMVDNTVPWKPGPVRALLASRPAAFQPLIDVERGAYGPKVGWLWDGHVA
                     VWLTTAKINKCSTIWTITDRDKSLPDHQTGQSGQALPPGTAFGRAPAYRFPGYLGFHI
                     VERWQFHYSQVVKSTR"
     gene            complement(2694..>3836)
                     /locus_tag="VFI55_15540"
     CDS             complement(2694..>3836)
                     /locus_tag="VFI55_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7075476.1"
                     /translation="LLGLALFGASSLACGAAPSMEILIAARAVQGVAGGLLAGLGYAV
                     INAALPRSLWTRASALVSAMWGVATLVGPAMGGLFAQFGLWRWAFVAMAILTVLMATL
                     VPFVLAAGRVDSNAATSTNVPVWSLVLIGAAALAVSVAQIPHNITATAGLLAIGVLLM
                     GLFVLVDWRTRAAVLPPSVFGPGPLKWIYLAMGALMIGAMVDTYVPLFGQRLAHLSPV
                     AAGFLGAALAVGWTVSEIVSASLEKSRTIGRVIAVAPLVVASGLALGAVTQRDNASVG
                     IVALWTLALLVAGVGIGMAWPHLSVRAMDSVSDPAEGVAAAAAINTVQLISGAFGAGL
                     AGVVVNTAGGGDVVAARWLFTVFTALTAGGVFASYRASYGAVRSKR"
BASE COUNT          650 a         1366 c         1195 g          625 t
ORIGIN      
        1 tgggcgaccg gtgcgccgcc gaacaggacg ggcccgatgg ccaaaacggc cgcgaccatc
       61 cgcagcgccg cgtcccgccc cccgcgactg gcgaacgtgt gtgtgcgcat cattggcgtc
      121 ctttcctttt ctcgagctga cgcgtcgacg tcgtgatgaa ttgagaaaag gctcgcaaag
      181 ggccctccac gatttctcaa caaaatcttg agccgcagct caccggccgg atcgccgcgg
      241 cgacgctaag gcagcccgca cccggaaacg gcccgttctc agcggggttg gcactgagtg
      301 ccgacccgtg gggaccgtcg actgaattcg tgcctaattc gtggtgtgca cgatcaacac
      361 gtcgaccttg gctcggcgcg agacgttggc gggcaccgat cccagcagcc ggcccgcgat
      421 cgtgctcagg ccgacgttac cgacgaccag cagatcggct ttttcgtctt cggccagctt
      481 gaccagcgcg tcgaccggcg cgccgacgat cgagcgctct tccacattct tggcgcccgc
      541 cgcgtgcgcc cgctccttgg catcctgcag gatcgcgtat atgggagcgg tgcccgacac
      601 cttgtagctt tcgtcccgca acgcgtcggc ggcccgggtg tcctcgtgct ggggcaggta
      661 cgccgacgcg atgaacaact tggcgtcggc cccggcgatt gcggccgccc gatccaccgc
      721 gcgcattgat gaatccgagc cgtcggtccc gaccaccacg gtccgatagg cgctcattta
      781 ccctccaagt ttcggttggt cagccaaccc aagacagtat cgcgtttgcc ggatgcacag
      841 gagaaagatt tgcgactcag cttggcgtgg cgcgaccggc tttcgcccca ccacgctgca
      901 tcaacgggca aatttcgtca gcgctagatc ggccgaatgc ccattgccaa ggcggaaagc
      961 cttgcattgc ccagcttttc gccaaacgcg ggcgaacacg ccatcaatgc accgtgcgcg
     1021 cgggcgctga tttcgaccgc ctgcgcgcaa agctgatggt gagccggttc atctcgcgcg
     1081 ccttacagtg acaagcatca tgtccatgcc cacccttgag cagcgcgccg cagacgtcgt
     1141 tgagcagcct gcgcccgcac tcgcacgccg cgggtcgctc gacgtgtggg cgattgccac
     1201 ggtcgccgcc gtcatcagcg gcgcctgggc cagtcggccg tcgttgtggt tcgacgaagc
     1261 ggccacgatt acggcttccg cgagccgcac ggtgcctgag ctgtggaaac tgatcggcca
     1321 catcgacgcc gtgcatggtc tctattactt cctgatgcac ggctggttcg cgctatttcc
     1381 gccgaccgaa ttctggtcgc gggtgcccag cgccctggcg gtcggggccg ccgccgcggg
     1441 tgtgacggtg tttaccaagc ggttcgtccc cgggcgtgcc accggggcct gcgccggcgt
     1501 catcttcgcc atcttgccgc gaaccacctg ggcgggtatc gaagcgcgtt cgtatgcgct
     1561 cgcggtcgcc gctgtcatct ggctgacggt gttgctcatc gccgcggtac gcaccaacag
     1621 gccgcgattg tggctgttct acgcgctggc attaatgctg tcggttttgc tgagcctcaa
     1681 cttgctgctg ttggtgccgg tttacgccgt gatggtgccg ctgttggccc gcacgaaatc
     1741 cccggtgatc aggtgggccg tcagctcggc cgtcgccgtc ggtgcgatga cgccgttcat
     1801 cgtcttcgcc cacggccaag tcggccaggt caactggatc tatcccgtca gctggcatta
     1861 cgccttcgac atcattttgc ggcagtattt cgaccacagc gttccgttcg ccgtgctcac
     1921 cgccgtgctc atcgtcgcgg ctgcggtggc ccggctgaag ggtgcgcgag gcccggcagg
     1981 cgacgtgcgc accctgttga ccgtctgcgc ggcgtggatc gtcattccga ccggcctcat
     2041 cgtcgcctac tcggccatca gtgaaccggt gtactacccg cggtacctga tcctgaccac
     2101 gcccgcgatg gccgtcatca tcgccgtctg catcgtgacg atcgcccgca agccgcggcc
     2161 catcgccgct gtcctgctgc tgttcgccct cgccgggttg ccggattacc tcttcacgca
     2221 gcgctggccg tatgccaaag agggctggga ttacagccag gtggccgacc tgatcagctc
     2281 ccatgccgca cccggggact gcctgatggt ggacaacacc gtgccctgga agcccgggcc
     2341 ggttcgcgcc ctgctggcca gccggccggc ggccttccag ccgctcatcg acgtcgagcg
     2401 cggggcttac ggacccaagg tcggctggct ctgggacggc cacgtcgccg tgtggctgac
     2461 gacggccaag atcaacaaat gctccaccat ctggaccatc accgaccggg acaagtcatt
     2521 gcccgatcac cagaccggcc aatccggaca agcactaccg ccgggcaccg cattcgggcg
     2581 tgcgccggcc taccgattcc cgggctacct cggattccac atcgtcgagc gctggcaatt
     2641 ccattactcg caggtcgtca agtccacgcg gtgatgggcg agccggactg aggtcagcgt
     2701 ttgctgcgca ccgcaccgta cgaagcgcga taactggcaa agacaccgcc ggcggtgagc
     2761 gcggtgaaca ccgtgaacaa ccatcgggcg gccaccacgt cgccgccccc ggccgtgttg
     2821 acgacaacac cggccaggcc ggcgccgaat gccccggaga tgagctgcac tgtgttgatc
     2881 gccgcggcgg ccgcgacgcc ttcggccgga tcgctgacgg aatccatcgc gcgcaccgac
     2941 aaatggggcc aggccatgcc gatccccacc cctgccacca gcaacgcgag cgtccagagc
     3001 gcgacgatgc cgaccgacgc gttgtcacgc tgggtgacgg cacccagcgc caggcctgac
     3061 gccaccacca gcggcgcgac ggcgatgacc cgcccgatcg tccgtgattt ctccaacgat
     3121 gcgctgacga tctcggagac cgtccagccg accgcgagcg ccgcacccaa aaatccggcc
     3181 gccaccggtg acaggtgcgc caaccgctga ccgaacaacg gcacgtaggt gtccaccatc
     3241 gcgccgatca tcagcgcccc catggccagg tagatccact tcagcggacc aggaccaaac
     3301 acgctgggcg gcaacaccgc cgcccgggtg cgccagtcga caagcacgaa caatcccatc
     3361 agcaagacac cgatggcgag cagcccggcg gtcgcggtga tgttgtgcgg gatctgggcg
     3421 acgctgaccg ccagcgcggc agcgccgatc aacaccagcg accacaccgg cacgttcgtc
     3481 gaagtcgcgg cgttcgagtc gacccggccg gcggccagca cgaacggcac caacgtggcc
     3541 atcagaacgg tcaggatcgc catcgcgacg aaggcccacc gccacaaccc gaactgcgcg
     3601 aacaggccac ccatcgcggg ccctaccagc gtggcgaccc cccacatcgc cgagaccagc
     3661 gccgaggccc gggtccacag cgagcgcggc agcgcggcgt tgatgaccgc atagccaagg
     3721 ccggcgagca ggccaccggc cacgccttgc acggcgcgtg ccgcgatgag gatctccatg
     3781 ctgggggctg ccccgcacgc caggctggag gcgccgaaca aggccaaccc caacag
//
LOCUS       DASSGQ010000351         5424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1058828,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000351 DASSGQ010000000
VERSION     DASSGQ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058828"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VFI55_15545"
     CDS             complement(<1..719)
                     /locus_tag="VFI55_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET7075477.1"
                     /translation="MSRPTPLHDLRVIEISDRIAGSYCGKLLVDAGAQVRKVEPPQGD
                     WLRRYTAGCSPVPEGAGSPLFSYLNAGKRSLTSAPGTERYRAELAAADVVVVTAGRSQ
                     AAALDIDPQRLLADSPRAIIVTISDFGWTGPYADRAASEFTLQAWAGSPGFRGDPAGP
                     PVATGGDLGEYMGGVFAAFGALAVRRRVEQGGRGEHLDLSVLEAITLMQSSEWLHSQL
                     LCVPPVRRTLEVPSIEPAKDG"
     gene            851..1684
                     /locus_tag="VFI55_15550"
     CDS             851..1684
                     /locus_tag="VFI55_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HET7075478.1"
                     /translation="MTALGIGPDARRRLSAPRIAEIVADELRRQIIDGELADGDLLPR
                     QEVLVDQFNVSLVSLREALRILETEGLVSVRRGNRGGAVVHAPAKTSAAYMLGLLLQS
                     KSVAVADLGMALQELEPACAALAAQRPDRADTLVPELKQVNDSMAEHLDDGRLFTEIG
                     RQFHDLIVRGCGNDTIIAVVGSLEALWTSHERQWADESAARGTYPSLAKRRAVLSTHI
                     KLADTIAEGDVDRARRIAGRHLADTQTYVLSGRPEQRIYALSPQALSRPGSRVQDVRH
                     S"
     gene            1705..2424
                     /locus_tag="VFI55_15555"
     CDS             1705..2424
                     /locus_tag="VFI55_15555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7075479.1"
                     /translation="MRTALVTGGSGGIGKGCGGKLSELGYDVVLVARREGPLRAAAQE
                     IGARHIVADVSDPEEFASAISTLPAIDLVVHAAGTLGGTYARKQTFEQWRDIMSANLD
                     SCFVVTAAALPRMRAGSRLVFISSSAAHDPMPARTAYSASKAGMNAFARALALEVDRD
                     GVSVHIVTPGPVETEMLQDVPFEMYAIQVSDVADAVAWLDTLDPSVDLPEIRLSAVQR
                     GPYARPPIVPTEARRRRRGTL"
     gene            complement(2387..3271)
                     /locus_tag="VFI55_15560"
     CDS             complement(2387..3271)
                     /locus_tag="VFI55_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7075480.1"
                     /translation="MVEWYLFLPQVRLSAADITERARHAEASGFDGMAFIDHLEAPGL
                     PDENIWEAMGIATWVAAKTERLRIGHLVLCDAFRHPAVLAKQAVTLSDASGGRFDLGL
                     GAGSWPAEFARFNVGQQDPVARVGQLKRNLSLIRQYWGDGTGDAAQAGRAAQAPRRSH
                     PIPLVLGGTGPRMMEIVRSYADWWNLPANHIDRLATLAPAAGTARLSVQQMVGFVRAG
                     GDPNAVREVSTRRFGNLGSGLVCGDADELAAHFAGLAARRVERFYVWFADFAAPDSVH
                     EFGESVIKAFPVAGDVPR"
     gene            3349..4425
                     /locus_tag="VFI55_15565"
     CDS             3349..4425
                     /locus_tag="VFI55_15565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7075481.1"
                     /translation="MDVGLTSAQLSLRDSVRDILRTECPTDAARQAITDPERWRALWK
                     TVVDLGWTELAAPDSDDYGPVELAVVLEECGAAIAPIPLLSSVGLAAGALRGTGVDSV
                     LTEIAGGVVATLAVHARGSRLPGAPMTLRHGHLRGRAVAVPNLSQAELIVTLARSDDG
                     VLVAVARRGDGVTALSGDSTDPAQPVADIEIDTQPLAAAPVHIESALTAPLVAAAADL
                     VGLASAALHRSVEHAKTRRQFGTPIGAFQGIKHALADNYVSVQRARSLTYAAAARLAD
                     AGTDPTGDRGAAWTAATLAKAAAGDAATSCARTAVQVHGALGQTWEHDVHLYVRHAWQ
                     GAAMLGDSRALYHQVGRRFAGGAG"
     gene            4422..>5424
                     /locus_tag="VFI55_15570"
     CDS             4422..>5424
                     /locus_tag="VFI55_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7075482.1"
                     /translation="MTAAVGEFGEWLAAFLPKDYYEKYRQYRWDLTLRRDYQRAAFEA
                     GWIQPTWPREHGGRSLGLRDAMEVRLEAATRLAPKLPNIAGPNVAAPGIRQFGTPAQI
                     ERLLVRVLRGDEWWALGMSEPEAGSDFAGLRTRAERDGDAFRVNGHKIWTTQAHESRW
                     CTLYVRTDPAAPKHRGISCLILDLTAPGVRIEPIRMASISDETFCEVFLDDVEVPAEN
                     LLGPLNGGWNVALSSLHHERQMIWIMNWVEIKRGLDSVRAASPNDDIYTELGSLLADA
                     EALRATGYRALGNELGGRPSPEADILKLLGSVTLQRVWELAAVAHGPQSTGDADLLFE
                     "
BASE COUNT          880 a         1883 c         1818 g          843 t
ORIGIN      
        1 tagccgtcct tggccggctc gatcgagggc acctcgagcg tgcggcgcac cggcggaacg
       61 cacaacagct gcgaatgcag ccattcgctg ctctgcatca aggtgatcgc ctcgagcacg
      121 gacaagtcga ggtgttcgcc acgaccgccc tgctcgacgc ggcggcgcac ggccagcgcg
      181 ccgaacgcgg cgaacacacc gcccatgtac tcccccaggt cgccaccggt cgcgacggga
      241 ggcccggcgg gatcgccacg aaagccgggc gagccggccc acgcctgcaa ggtgaactcg
      301 ctggccgcgc ggtccgcata cggtccggtc cagccgaagt cggagatcgt cacgatgatc
      361 gcccgcgggg aatcggccag cagccgctgc ggatcgatgt ccagcgccgc ggcctgcgac
      421 cggccggcgg tgacgaccac gacgtcggcg gcggccagtt cggcgcggta gcgttcggtg
      481 cccggcgccg aagtcaggct ccgcttgccg gcgttgagat agctgaacaa cggcgaacct
      541 gcgccttccg gcaccggcga acacccagcg gtgtaccgtc gcagccaatc cccttgcggc
      601 ggttctactt tgcgcacctg cgccccggcg tcgacgagca gcttcccgca gtagctgccg
      661 gctatccggt cactgatctc gataacacgc aagtcgtgca gcggtgtcgg tcggctcact
      721 tgcacccgcc ctcccgatcg gcgtgcgatc gcaagacgct atttatacca ttactgctaa
      781 aattgctaag ccagtagagc gcgcggctag gttccgcgag ctctttccta ttgtttgcca
      841 cgaggattgg atgaccgccc tgggaattgg ccccgatgcc cgtcgccgat tgtcggcgcc
      901 gcggatcgcc gaaatcgtag ccgacgagtt gcgccgtcag atcatcgacg gcgagctcgc
      961 cgacggcgat ctgctgcctc gccaagaggt gctcgtcgac cagttcaacg tcagtttggt
     1021 gtccctgcga gaagcgctgc ggatcttgga aaccgagggc ctggtgtcgg tccggcgggg
     1081 caaccgcggc ggcgccgtcg tccatgcgcc ggcgaagacc agcgcggcct acatgctggg
     1141 tctgctgctg cagagcaagt ccgttgcggt cgccgacctg ggcatggcgt tgcaagaact
     1201 cgaacccgcc tgcgccgcac tggccgccca gcgccccgat cgggcggaca ccctggttcc
     1261 agaactcaag caggtcaacg attccatggc cgagcatctc gacgatggcc ggctcttcac
     1321 tgaaatcggg cgccagttcc acgatctcat cgtccgcggc tgcggaaacg acaccatcat
     1381 cgcggtcgtc ggcagcctcg aggcgctgtg gaccagccac gaacggcagt gggcggacga
     1441 gagcgcggcg cgcggcacct acccttcgct ggccaaacgc cgcgccgtgc tcagcacgca
     1501 catcaagctc gccgacacca tcgccgaggg cgacgtcgac cgggctcgcc gcatcgccgg
     1561 ccgccacctc gccgacacgc agacctatgt gctgtccggt cggcccgagc aacggatcta
     1621 tgcgctttca ccgcaggctc tgtcacgtcc ggggtcgcgc gtgcaagatg tccggcactc
     1681 ctagcgggga agcccctaac gtccatgcga accgcattgg tcaccggcgg tagcggcggg
     1741 atcggaaagg gatgcggcgg caagctgagt gagctgggtt acgacgtggt gctggtggcg
     1801 cggcgcgaag gcccgctgcg cgccgccgcc caggagatcg gtgcgcgcca tatcgtggcc
     1861 gacgtctccg atccggagga attcgcttct gcgataagca ctttgccggc gattgatctg
     1921 gtggttcacg cggcgggcac gctgggcggg acctacgcgc gcaagcagac attcgagcag
     1981 tggcgagaca tcatgtcggc caatctggac tcgtgcttcg tggtgaccgc cgccgcgctg
     2041 ccacgcatgc gcgcgggctc gcggctggta ttcatctcgt cgtcggcagc tcatgacccg
     2101 atgccggccc gcaccgccta ctccgcatcg aaagcgggca tgaacgcgtt tgcgcgggcc
     2161 ttggcgctgg aggtcgatcg ggacggcgtc agcgtgcaca tcgtcacgcc gggcccggtg
     2221 gaaaccgaga tgctgcagga cgttcctttc gagatgtacg caattcaggt gtccgacgtc
     2281 gcggacgcgg tcgcgtggct ggacaccctc gacccgtcgg tcgacctgcc ggagatccgg
     2341 ctcagcgcag tgcagcgagg cccctacgcg cgaccgccga tcgtgcctac cgaggcacgt
     2401 cgccggcgac ggggaacgct ttgatcaccg attcgccgaa ctcgtgcacg gaatccgggg
     2461 cggcgaaatc ggcgaaccac acgtagaacc gctccactcg ccgggcggcc aggccggcga
     2521 aatgtgcggc cagctcgtcg gcatcgccgc agaccagccc cgaccccaag ttgccgaatc
     2581 gccgggtgct cacttcgcgc acggcgttgg gatcaccgcc cgcccgtacg aagcccacca
     2641 tctgctggac cgatagccgg gcggttcccg ccgccggtgc cagcgtggcc aacctgtcga
     2701 tatggttcgc cggcagattc caccaatccg catagctacg aacgatttcc atcatccggg
     2761 gcccggtacc gcccaagacc agcggaatcg gatgggacct tcgcggagct tgcgcggcgc
     2821 ggccggcctg cgcggcatcc cccgtcccgt cgccccagta ctgccggatc aggcttaggt
     2881 tgcgtttgag ttggccgacc ctggccaccg gatcctgctg accgacattg aatctggcga
     2941 actcggcggg ccaggatccc gcgccgaggc cgagatcgaa gcggccgcct gacgcgtccg
     3001 agagagtgac ggcttgcttg gcgagcacgg cggggtgccg aaaggcgtcg cagagaacca
     3061 ggtggccgat ccgtagccgc tccgtcttcg ccgccaccca ggtggcgatg cccattgcct
     3121 cccagatgtt ttcgtcgggc agcccgggcg cctccaggtg atcgatgaac gccattccat
     3181 cgaaaccgct ggcctcggcg tggcgcgccc gttccgtgat gtcggcagcc gacagccgca
     3241 cctgcggaag gaacagatac cactcgacca cgcgaccacc tgtgctttgc gttccctgcg
     3301 attgcagcgg tgtcgctagt ttactatttt tattatgtta ggggtcttat ggacgtcgga
     3361 ctgacttcag cgcaactgtc gctgcgcgac agcgtgcgcg acatcctgcg caccgaatgt
     3421 cccactgacg ccgcgcgcca ggccatcacg gaccccgagc gctggcgcgc gctgtggaag
     3481 acggtcgtcg acctcggctg gacggaactt gcggcgcctg actccgacga ctacggaccc
     3541 gtcgagctgg cggtggtact cgaggaatgt ggcgccgcca tcgcgccaat cccgttgctc
     3601 agcagcgtcg ggctggccgc gggcgcgctg cgcggcacgg gcgtcgactc agtgctcacc
     3661 gagattgccg gcggcgtcgt cgccaccctg gccgtccacg ccagaggatc gcggctccct
     3721 ggcgcaccga tgacgctgcg tcacggacat ctgcgcggac gcgcggtcgc ggtgcccaac
     3781 ctgtcgcagg ccgagctgat cgtcacactg gcacgatccg acgacggcgt gctggtcgcc
     3841 gtcgcacgcc gcggcgacgg ggtgaccgcg ctgtccggcg actcgacgga ccccgcacaa
     3901 cccgtggccg acatagagat cgacacacag ccgctggccg ccgcgcccgt gcacatcgaa
     3961 tcggcgttga cggcaccgtt ggtggccgcg gccgccgacc tggtcggcct ggcgagcgcg
     4021 gccctgcacc gctcggtcga gcatgcgaag acgcgtcgtc aattcggcac accgatcggc
     4081 gcgttccagg gcatcaaaca cgcgctggcc gacaactacg tcagcgtgca acgcgcgcgc
     4141 agcctcacct acgcggccgc ggcccggctc gccgacgccg gcaccgaccc caccggggac
     4201 cgcggcgccg cctggaccgc cgcgacgctg gccaaggcgg cggccggcga cgcggccacc
     4261 agttgtgcgc gcaccgcggt tcaggtgcac ggcgccctcg ggcagacctg ggagcacgac
     4321 gtccacctct acgtccggca cgcctggcag ggcgccgcga tgctgggcga tagccgcgcg
     4381 ctctaccacc aggtgggccg ccgtttcgca gggggcgcgg gatgacggcg gcggtcggcg
     4441 aattcggcga gtggttggcg gcgtttctgc cgaaggatta ctacgagaag tatcgccagt
     4501 accgctggga cctgacgctg cggcgtgact accagcgggc cgccttcgag gccggctgga
     4561 tacagccgac ctggccccgc gagcacggcg gacgatcatt gggtttgcgc gacgcgatgg
     4621 aggtccggct cgaggcggcg acgcggttgg cgcccaagct gcccaacatc gccggtccca
     4681 acgtcgccgc acccgggatc cgtcagttcg gcacacccgc ccagatcgag cggctgctgg
     4741 ttcgggtgct gcgcggtgac gagtggtggg cgctgggcat gtcggagccg gaagccggat
     4801 cggacttcgc cggcctgcgc acacgtgccg agcgcgacgg ggacgcgttc cgggtcaacg
     4861 gtcacaaaat ctggaccacc caggcccacg agtcgcggtg gtgcaccctg tatgtccgca
     4921 ccgacccggc ggcgcccaaa catcgcggta tctcttgcct gatcctcgac ctgactgcgc
     4981 ccggggtgcg gatcgagccc atccggatgg cgtcgatctc cgacgagacg ttctgcgagg
     5041 tgttcctcga cgacgtcgag gtgccggcgg agaacctgct ggggccactc aacggcggct
     5101 ggaatgtcgc gttgtcgtcg ctgcaccatg agcgtcagat gatctggatc atgaactggg
     5161 tcgaaatcaa gcgcggactc gactcggtcc gtgcggcgag tccaaacgac gacatataca
     5221 ccgagctcgg ctcgctgctc gccgacgccg aggccctgcg ggccaccgga taccgtgcgc
     5281 tgggcaacga actgggtggg cgacccagcc cggaagccga catcctgaaa ctgctcggat
     5341 cggttacctt gcaacgggtt tgggaactcg ccgccgtcgc ccacggacca cagtcgacag
     5401 gcgacgccga tttgctcttc gaac
//
LOCUS       DASSGQ010000352        10058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_238156,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000352 DASSGQ010000000
VERSION     DASSGQ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10058
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238156"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /gene="bfr"
                     /locus_tag="VFI55_15575"
     CDS             complement(<1..398)
                     /gene="bfr"
                     /locus_tag="VFI55_15575"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HET7075483.1"
                     /translation="MQGDPDVLRLLNEQLTSELTAINQYFLHSKMQDNWGFTELAAHT
                     RAESFDEMRHAEAITDRILILDGLPNYQRLFSLRIGQTLREQFEADLAIEYEVLQRLK
                     PGIIMCRAKEDSTSAILLEKIVADEELHVD"
     gene            707..892
                     /locus_tag="VFI55_15580"
     CDS             707..892
                     /locus_tag="VFI55_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7075484.1"
                     /translation="MYVCLCVGATNQTVCDAVARGACTSKQVAAACGAGGDCGRCRRT
                     LRAIIAAARQLEPAPSA"
     gene            complement(911..1663)
                     /locus_tag="VFI55_15585"
     CDS             complement(911..1663)
                     /locus_tag="VFI55_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET7075485.1"
                     /translation="MILQIDDDKRVRTLTLNRPEALNAFNEALYDATAEALLAAADDP
                     EVAVVLLTGNGRGFSAGTDLAEMQARISDPTFTPGKHGFTGLIDALSAFPKPLICAVN
                     GVGVGIGATILGYADLAFMSSTARVKCPFTSLGVAPEAASSYLLPRLVGRQNAAWLLM
                     SSEWVDADEALRMGLVWRVCDPDELLPEARRHAEILAAQPISSLMAVKHTMMEPIRPE
                     IAAASARENAHFAELMGAQANAAALADFTKPR"
     gene            complement(1719..2162)
                     /locus_tag="VFI55_15590"
     CDS             complement(1719..2162)
                     /locus_tag="VFI55_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HET7075486.1"
                     /translation="MTTLRDSVALAAQEHGLAVVSTVRADATVQASLVNVGLLPHPAT
                     GEPVLGFTTYGKVKLANLRARPQLAVTFRNGWQWATVEGRAELAGPDDTRPWLKDADQ
                     LRLLLREVFTAAGGTHDNWDEYDRVMAKERRAVVLIAPSRVYSNG"
     gene            complement(2172..2873)
                     /locus_tag="VFI55_15595"
     CDS             complement(2172..2873)
                     /locus_tag="VFI55_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017487708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075487.1"
                     /translation="MEKVIAVLMRAESDDDWCARQRGPVAEALLNLGIPGLSVNVRDD
                     AVRHSLMTLTTLDPPVAAVVGLWTQQCYGDQMAAALTLLDAECENLAAYLVTESVPLT
                     APPTEPGCRTTGLANIALLRRPAGLDEATWLTRWQRDHTSVAIETQSTFGYTQNWVVR
                     NLTPNAPGIAGIVEELFPAEAITDLRAFFGAADDNDLQNRISQMVASTTAFGANENID
                     TVPTSRYVFKTPFTD"
     gene            2982..4304
                     /locus_tag="VFI55_15600"
     CDS             2982..4304
                     /locus_tag="VFI55_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HET7075488.1"
                     /translation="MTQAPPGTSWVQHAIWWQVYPLGFVGAFPTASEPPQPAEHRLRR
                     LVGWFDHAIALGASGIALGPIFASRTHGYDTTDHYRIDPRLGDDADFDHLVAEAHRRG
                     LRVLLDGVFNHVGVDFALYRDAEHDDAAARWFRVGPDRFHTFEGHSELITLNHDNPEV
                     VDYTVDVLAHWLRRGADGWRLDAAYAVPERFWATTLPRVHEQHPDAWFVGELIHGDYA
                     AVVEAAKFDSATQYELWKAIWSSLNDGNFFELDWALQRHNAFVASFAPLTFIGNHDVT
                     RIASRLDHRGHGAHALVILLTVGGVPSIYAGDELGFCGVKEERYGGDDAVRPEFGSPP
                     LELDASGTEVWALHQYLVGLRRRHPWLHAASTTALRLDNRHYVYETRSGDNVLLVALN
                     IDDEPLTLALSEFGAARARVLAGSAAPPDEVVDTMVVEPHGWRILSPV"
     gene            complement(4301..4738)
                     /locus_tag="VFI55_15605"
     CDS             complement(4301..4738)
                     /locus_tag="VFI55_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HET7075489.1"
                     /translation="MEWASDPFDLKRFVDAQAPVFRTVIDELRGGRKRGHWMWFVFPQ
                     LRGLGSSPMAERYGISSLEEARAYLRHDLLGPRLRECTGLVNQVQGRSVAEIFGSPDD
                     LKLCSSMTLFARATDDNADFVALLDRYYGGRHDPVTVTRLTAP"
     gene            4757..5998
                     /locus_tag="VFI55_15610"
     CDS             4757..5998
                     /locus_tag="VFI55_15610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HET7075490.1"
                     /translation="MAVKRRVPTMRDLAPLIQFKKPVLDSTKRRLDAALTIQDLRRIA
                     KRRTPKAAFDYADGAAEDELSIERARQAFRDIEFHPAILRDVTNVTAGWNVLGQPVVL
                     PFGIAPTGFTRLMHTEGEIAGAQAAARAGIPFSLSTLGTTAIEDLVTAVPQGRKWFQL
                     YMWRDRERSMALVQRAADAGFDTLLATVDVPVAGARLRDSRNGMTIPPTLTLRTVLDA
                     LPHPKWWFDLLTTEPLSFASLDRWPGTVGEYLSTMFDPSLTFDDLEWIKAQWPGKLVV
                     KGIQTLDDARAVVERGVDGIVLSNHGGRQLDRAPVPFHLLPAVARELSRDTEILVDTG
                     IMAGADIVAAIALGARCTLIGRAYLYGLMAGGEAGVNRAIEILQSGIIRTMALLGVTC
                     LEELSPHHVTQLQRLGPLPSA"
     gene            6184..8502
                     /gene="lon"
                     /locus_tag="VFI55_15615"
     CDS             6184..8502
                     /gene="lon"
                     /locus_tag="VFI55_15615"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET7075491.1"
                     /translation="MTEAKSVPVLFTSTIVLPGMVVPIALDDAARAAIDAAQASESGE
                     LLIAPRLDDRYPSYGVIAKILQVGRIAGGGTAAVVRGERRAHIGAGASGPGAALWVEV
                     TEVPATQATDEVKALAAEYKKLLLAMLQRREAWEIVDYVNGLTDPSALADTSGYASYL
                     TDVQKRQLLETVDVAERLRVLIEWTSSHLAEVEVNDKIAEDVREGMEKTQKEFLLRQQ
                     LAAIRKELGEGEPDGSDDYRARVEAADLPEKVREAALREVGKLERASEQSPESGWIRT
                     WLDTVLDLPWNVRTEDSTDLSAAREILDADHHGLDDVKDRIVEYLAVRTRRAQRGLQV
                     VGGRGSGAVMVLAGPPGVGKTSLGESVARALGRKFVRVALGGVRDEAEIRGHRRTYVG
                     ALPGRIVRAIGEAGTMNPVVLLDEIDKVGSDYRGDPSAALLEVLDPAQNHTFRDHYLD
                     LDLDLSDVVFLATANVIENIPSALLDRMELVQIDGYTEDDKVAIARDYLLPRQRERAA
                     LSEDEVVVTDAALRKIAADYTREPGVRQFERLLAKALRKVTTKLAEDSGPKTIDEPDL
                     VGYLGRPRFTPESAERTAVPGVATGLAVTGLGGDVLYIEAGATDGEPGLQLTGQLGDV
                     MKESAQIALSYVRSHASELGVDPKALDRRIHVHVPAGAVPKDGPSAGVTMVTALVSMA
                     TGRQVRSDVGMTGEVTLNGRVLPIGGVKQKLLAAQRAGLATVFIPARNEPDLDDVPAE
                     VLDALTVKPMTDVAEIVAQALEPATLTVTDAA"
     gene            8585..8974
                     /locus_tag="VFI55_15620"
     CDS             8585..8974
                     /locus_tag="VFI55_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET7075492.1"
                     /translation="MNGTMSFKRELVHRVQRMVVNPIGRQLPVTMLETTGRKSGRPRH
                     TAVGGKVVDNQFWMVSEHGEHSDYVYNIKANPAVRVRINGQWRNGTAHLLPDDDPRQR
                     LRSLPRLNSAGVVTMGTDLLTIRVDLV"
     gene            8985..9317
                     /locus_tag="VFI55_15625"
     CDS             8985..9317
                     /locus_tag="VFI55_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HET7075493.1"
                     /translation="MAYDEDLANRIRALLGSERGVEEKRMFGGLALLINGNMSVAASS
                     KGGLMVRVPPEDTDKLLARAHVSPMVMAGRETRGWLRVDADGLGTKRQLQSWVTRGVG
                     YARSLPPK"
     gene            9440..>10058
                     /locus_tag="VFI55_15630"
     CDS             9440..>10058
                     /locus_tag="VFI55_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7075494.1"
                     /translation="MASSTTFVIVGGGLAGAKAVEALRDNDFDGQIILFADEGYPPYE
                     RPPLSKEYLAGKKTLADFTVHNSDWYREHKVDVRLGSRVSSLDPAAHTVGLPDGTTAA
                     YHKLLLATGSASRRPPIPGSDADGVYYLRTYDDAAALNSVLTEGSSLAVVGAGWIGLE
                     VAAGARQRGVGVTVVEAAKQPLAAALGETVGEVFATLHRDHGVDLR"
BASE COUNT         1651 a         3265 c         3493 g         1649 t
ORIGIN      
        1 tagtcgacgt gcagctcctc gtcggccacg atcttctcca ggaggatggc gctggtgctg
       61 tcctccttgg cgcggcacat gatgattccg ggcttcaggc gttgcagcac ctcgtattcg
      121 atcgccaggt cggcctcgaa ttgctcgcgc agcgtctggc cgatccgcaa cgagaagaga
      181 cgctggtagt tcggcaggcc gtctaatatc aggatgcgat cggtgatcgc ctcggcgtgc
      241 cgcatttcgt caaaggactc ggcacgggtg tgtgcggcca actcggtgaa tccccagttg
      301 tcctgcatct tggaatgcag gaaatattgg ttgatcgcgg tgagctcact ggtcagctgc
      361 tcgttgagca ggcgcaaaac atccgggtca ccttgcatgt cactcctagg cgtgtgaagg
      421 ccgtcatgtc caaccgcggc gggtgtgctg ggctgaagca caccacgctg ggttgtagca
      481 gcacgttgcc atgccggctt cgaatctaat gcagcgggtc tgggtcgacg cggtgtttaa
      541 ccacccatcg gcgcgtcagt caacgggcag ccggccgacg caccgcaaac ttaggttagg
      601 tacacctacg ctgacttatg ttaggctcta ctaacatgcg gccggttcgc tccctaatcc
      661 cctggcgtga agcaatgccg gctgcccgcg cgcggagggt gtgctgatgt acgtgtgcct
      721 gtgcgtcggg gccaccaatc agacggtgtg cgacgccgtc gcccggggtg cgtgcacgtc
      781 caagcaggtt gccgctgcgt gcggcgccgg cggtgactgc gggcgctgcc ggcgcacgct
      841 acgcgcgatc atcgccgcgg cgcggcaact cgagcccgcc ccgtcggcat agcgacacag
      901 cccggcgggg tcagcgaggt ttggtgaagt ccgccagggc cgcggcgttg gcctgggcgc
      961 ccatcagttc ggcaaagtgg gcgttttccc gcgcgctggc cgcggcgatc tcgggacgga
     1021 tcggctccat catcgtgtgc tttaccgcca tcaggctcga aatcggctgc gcggcaagga
     1081 tttcggcatg ccgacgggcc tcggggagca actcgtccgg gtcgcacacc cgccagacca
     1141 ggcccatccg cagcgcctcg tcggcgtcga cccattccga ggacatcaac agccaggcgg
     1201 cgttttgccg cccgaccagc cgcggcagca ggtatgacga cgcggcctcg ggcgccacgc
     1261 ccaggctggt gaagggacat ttcacgcgcg ccgtcgacga catgaatgcc agatcggcgt
     1321 acccgaggat ggtggcccca atccccaccc cgacaccgtt gacggcgcag atcagtggct
     1381 tgggaaacgc gctcagcgca tcgatcaggc ccgtgaagcc gtgctttccg ggcgtgaagg
     1441 tcgggtcgct gatgcgcgcc tgcatctccg cgagatcggt gccggcgctg aaaccgcggc
     1501 cgttgccggt gagcaggacg acggcgacct ccggatcgtc ggccgcagcc aacagcgcct
     1561 ccgccgtggc gtcgtagagg gcttcgttga acgcgttgag cgcctcaggc cggttcagcg
     1621 tgagggtgcg gacccgtttg tcgtcgtcga tctgcaggat cacggctgca gcctaacccg
     1681 gttggggcag gccggaacgg tccgcgccgg ccgggggttc agccgttgct gtacacgcgg
     1741 gagggcgcga tcagcacgac cgcgcgccgt tccttggcca tcacccggtc gtactcgtcc
     1801 cagttgtcgt gggtgccgcc ggcggcggtg aagacctcgc gtagcagcag ccgcaactgg
     1861 tcggcgtctt tcagccaagg ccgcgtgtca tccgggccgg ccagctccgc gcggccctcg
     1921 acggtcgccc actgccatcc gttgcgaaag gtgaccgcca gctgcggccg ggcccgcagg
     1981 ttggccagtt tcactttgcc gtaggtggta aagcccagaa ccggctcacc ggttgcgggg
     2041 tgcggcagca gaccgacgtt gaccagcgag gcctgcaccg tcgcatcggc acgcacggtg
     2101 gataccaccg cgagaccgtg ttcttgggcg gccagcgcca ccgaatcgcg cagtgtcgtc
     2161 atatcagttc ctcaatctgt gaacggcgtc ttgaagacgt agcggctggt cggcacggtg
     2221 tcgatgttct cgttggcacc gaatgcggtc gtgctggcta ccatctggct tatccggttc
     2281 tgcaggtcgt tgtcgtcggc ggcaccgaag aacgccctca gatcggtgat cgcctctgcg
     2341 gggaacaact cttcgacgat gcccgcgatt cccggcgcgt tcggggtcag gtttcgtacc
     2401 acccagttct gggtgtagcc gaacgtggac tgtgtctcga tggccaccga cgtgtggtcg
     2461 cgctgccatc gggtcagcca ggtcgcctcg tccaggccgg cgggccggcg cagcagggca
     2521 atattggcca aaccggttgt ccgacaacct ggttcggttg gcggtgcagt cagcgggacc
     2581 gactcggtga ccaggtaggc ggcgagattc tcacattcgg cgtcgagcag ggtcagtgcc
     2641 gccgccatct ggtcgccata acactgctgg gtccacaggc cgaccaccgc cgcaactggg
     2701 gggtccaggg ttgtcagcgt catcagcgag tggcgcacgg cgtcgtcgcg aacgttgacc
     2761 gacagccccg ggatgcccag gttcagcagc gcctcggcga ccggcccccg ttgccgggcg
     2821 caccaatcgt cgtcggattc ggcccgcatc aacacggcga tcaccttctc catacagccg
     2881 aatttactgc cggtaggact cgtcgcgacc gctggacgag cggcggcggc acgccggcgt
     2941 aacgtcgacg aggtgttgcg ggtgctgctc aagaagtcgg catgactcag gccccgcccg
     3001 gaacctcctg ggttcagcat gcgatctggt ggcaggtcta tcccctgggc ttcgtcggcg
     3061 cgtttcccac cgcgtcggag ccgccccagc cggccgaaca ccggctgcgg cggctcgtcg
     3121 gctggttcga ccacgccatc gcactggggg cgtccggcat cgcgttggga ccgatcttcg
     3181 cctcgcgcac acacggttac gacaccaccg atcactaccg catcgatccc cggctcgggg
     3241 acgacgccga cttcgaccac ctcgtcgcgg aagcgcatcg ccgcgggctg cgtgtgctgc
     3301 tcgacggcgt gttcaatcac gtgggcgtgg acttcgcgct ctatcgcgac gcggagcacg
     3361 acgacgcggc ggcgcgctgg tttcgcgtgg gtccggatcg gtttcacacc ttcgagggcc
     3421 actccgagct catcaccctc aatcacgaca accccgaggt ggtcgactac accgtcgacg
     3481 tgctggcgca ctggctacga cgcggtgcgg acggctggcg cctggatgcg gcttacgctg
     3541 tgccggaacg attctgggcg acgacgctgc cgcgggtaca cgagcagcat cccgacgcat
     3601 ggttcgtcgg cgagctcatc cacggcgact acgcggcggt ggtcgaggcg gccaaattcg
     3661 actcggccac ccaatacgag ctgtggaagg cgatctggag cagcctcaac gacggcaact
     3721 tcttcgagct ggactgggcg ctgcagcggc acaacgcgtt tgtggcgagt tttgccccgc
     3781 tgacgttcat cggcaaccac gacgtcaccc gcatcgccag ccggctcgac caccgcggac
     3841 atggggccca tgcgctggtg atcctgctga ccgtcggcgg cgtgcccagc atctacgccg
     3901 gcgacgagct cgggttttgc ggcgtcaagg aagagcggta cggcggggac gacgcggtgc
     3961 gacccgagtt cggctctccc ccactggagt tggacgcgtc cggcaccgag gtgtgggccc
     4021 tgcaccagta cctcgtcggg ctgcgccgtc gccacccgtg gctgcacgcg gcctcgacga
     4081 cggcgctgcg gctggacaat cgacactacg tctacgagac ccgcagtgga gacaacgtgc
     4141 tgctggtcgc gctcaacatc gacgacgagc cgctgaccct ggcgctgtcg gagttcggcg
     4201 cggcacgggc acgggtgctt gctggatcgg cggccccacc cgacgaggtg gtggacacca
     4261 tggtggtgga gccccacggc tggcggatcc tgagccccgt ctagggggcc gtcagccgcg
     4321 tcaccgtcac cgggtcatgc cggccaccgt agtaacggtc gagcagcgcg acgaagtccg
     4381 cgttgtcgtc ggtggcacgg gcgaacaacg tcatcgacga gcacagcttg aggtcgtcgg
     4441 gggaaccgaa gatctccgcg accgaccgac cttgcacctg gttgaccagc ccagtgcact
     4501 cgcggagccg gggcccgagc aggtcgtgtc gcagatacgc gcgtgcttcc tcgagtgagg
     4561 aaatgccgta gcgctccgcc atcgggctgc tgcccaaacc gcggagctga gggaacacga
     4621 accacatcca gtgcccgcgt tttcgtccgc cgcgcagctc gtcgatgacg gtgcggaaga
     4681 ccggagcctg cgcgtccacg aaccgcttca gatcgaaggg gtcgctcgcc cattccatgt
     4741 gactaccgtt gcacatatgg cagtcaaacg acgagtgccc acgatgcgtg atctcgcgcc
     4801 gttgatccag ttcaaaaagc ccgtgctgga tagcaccaaa cgccggctgg atgccgcgct
     4861 gaccatccag gacctgcgac gcatcgccaa acggcgcacg cccaaggcgg cattcgacta
     4921 cgccgacggc gccgccgagg acgagctgtc catcgagcgc gcccgacaag ccttccgcga
     4981 catcgagttt cacccggcga tcctgcgtga cgtcactaac gtgaccgccg gctggaacgt
     5041 cctgggccaa cccgtcgtcc tgccgttcgg gatcgcgccg accggattca cccggttgat
     5101 gcacaccgag ggcgagatcg ccggcgcaca ggcggcggcc agggcgggga tcccgttttc
     5161 gctctccact ctgggcacca ccgcgatcga agacctggtg accgcggttc cccagggccg
     5221 caagtggttt cagctgtaca tgtggcgcga tcgcgaacgt tcgatggcgt tggtgcagcg
     5281 ggcggccgac gcgggattcg acaccctgct ggccacggtc gacgttccgg tcgcgggtgc
     5341 gcgcttgcgc gatagccgca acgggatgac gatcccgccg acgctgacgt tgcgaaccgt
     5401 gctcgacgcg ctgccccacc cgaagtggtg gttcgatctc ctgaccaccg agccgctgtc
     5461 gttcgcgtca ttggatcgct ggccgggcac cgtcggtgag tacttgagca ccatgttcga
     5521 tcccagcctc accttcgatg acttggagtg gatcaaggct caatggccgg gcaagctcgt
     5581 ggtcaagggg atccaaacgc tcgacgacgc gcgggccgtc gtcgagcgcg gcgtcgacgg
     5641 catcgtgctg tccaatcacg gtggccgcca actagaccgg gcgccggtgc ccttccacct
     5701 gctgcccgcg gtggcgcgcg agctgagtag ggacaccgag atcctggtgg acaccggcat
     5761 catggccggc gccgacatcg tggcggcgat cgcgctgggg gcccgctgca cgctgatcgg
     5821 acgggcctat ctgtatgggc tgatggccgg cggtgaggcc ggtgtcaacc gcgcgatcga
     5881 gatcctgcag agcgggatca tccggacgat ggcactactc ggtgtcacct gcctcgagga
     5941 actctcgccc caccacgtga cgcagctgca gcggctgggt ccgcttcctt cagcgtgacc
     6001 ggattgcgtt aggcctccgg ccccgtcgcc agcgccgtca tcagcgccca aattgccgtg
     6061 agggctgtgg ttcgggcgcg aatacgaccg tgtcggcaat ctcgttgttt ttggacttcc
     6121 cgtccgggaa caaaccggcg gtcagctggg ttaagcgcat cagactcaag ttttggagga
     6181 ttgatgaccg aagccaaatc agtgccggtg ttgttcacca gcacgatcgt gttgccagga
     6241 atggtggtgc cgatcgcgct ggacgacgcc gcccgggcgg cgatcgatgc cgcgcaggcc
     6301 agtgaatccg gggaactgct gatcgctccc cggctggatg accgttaccc gtcgtacggc
     6361 gtgatcgcga agattctgca ggtcggacgc attgccggcg gtggaaccgc ggcggtggtg
     6421 cgcggcgagc gacgcgcgca catcggagcg ggggcttccg ggcccggggc ggcgctgtgg
     6481 gtcgaggtga ccgaggtgcc cgcgacccag gccactgacg aggtcaaggc cctggcggcg
     6541 gagtacaaga agctgctgct ggccatgctg caacggcgcg aggcctggga gatcgtcgac
     6601 tacgtcaacg gcctgaccga cccgtcggcg ctggcggaca cgtccggtta cgcgtcctac
     6661 ctgaccgatg tgcagaagcg gcagctgctg gaaacggtgg acgtcgccga gcggttgcgg
     6721 gtgctgatcg agtggaccag ctcgcatttg gccgaggtcg aggtcaacga caagatcgcc
     6781 gaggacgtgc gggaaggcat ggagaagacg cagaaggagt tcctgctgcg tcaacagctg
     6841 gccgccatcc gcaaggagct gggcgagggc gaacccgacg gatccgacga ctaccgggcc
     6901 cgggtggagg ccgccgacct gcccgaaaag gtgcgggaag cggcgctgcg cgaggtcggg
     6961 aaactggaac gggccagcga gcaaagcccg gaaagcggct ggatccgcac ctggctggac
     7021 accgtgctcg acctgccgtg gaacgtgcgg accgaggact cgaccgacct cagcgcggcc
     7081 cgggaaattc tggacgccga ccaccacggg ttggacgacg tcaaggaccg catcgtggaa
     7141 tacctggccg tgcgcacccg ccgcgcccaa cgtgggctgc aggtggtcgg cggccgcggc
     7201 tcgggcgcgg tgatggtgct ggccggtccg ccgggcgtcg gcaagacctc gctgggtgag
     7261 agcgtggccc gggcgctggg ccgcaagttc gtgcgggtcg cgctgggcgg tgtgcgcgac
     7321 gaggccgaga tccgcgggca ccggcgcacc tatgtgggtg cgctgccggg ccggatcgtg
     7381 cgtgcgatcg gcgaggcggg gacgatgaat cccgttgtgc tgctggacga aatcgacaag
     7441 gttggttccg attaccgggg cgacccgagt gcggcattgc tggaggtgct cgacccggcg
     7501 cagaaccaca cgttccgcga tcactacctg gatctggacc tggacctgtc cgatgtggtg
     7561 ttcctggcca ccgccaacgt gatcgagaac atcccgtcgg cgttgctgga ccgcatggag
     7621 ctggtgcaga tcgacggcta cacggaggac gacaaggtcg ccatcgcccg tgactacctg
     7681 ctgccgaggc agcgggagcg ggcggcgctg agcgaggacg aggtcgtcgt gaccgacgcc
     7741 gcgctgcgca agatcgccgc cgactacacc cgtgagccgg gggttcggca gttcgagcgg
     7801 ctgctggcca aggcgctgcg gaaggtgacg accaagctcg ccgaggacag tgggccgaag
     7861 acgatcgacg agcccgatct ggttggctac ctgggccggc cgcgtttcac gcccgagtcg
     7921 gccgaacgga ccgcggtgcc cggcgtggcc accggtctgg ccgtcacggg tctgggcggc
     7981 gatgtgctct acatcgaagc cggggccacc gacggtgagc cgggtctgca gttaaccggt
     8041 caactgggcg acgtgatgaa ggagtcggcg cagatcgcct tgtcgtacgt gcggtcgcac
     8101 gcctcggagt tgggcgtcga tcccaaggcg ctggaccggc gcatccacgt gcacgtgccc
     8161 gcgggcgcgg tgcccaagga tggcccgtcg gccggggtga cgatggtgac ggccctcgtc
     8221 tcgatggcga cggggcgtca ggtgcgctcc gacgtgggca tgaccggcga ggtcacgctg
     8281 aacggccggg tgctgccgat cggcggcgtg aagcagaagc tgctggccgc ccagcgtgcc
     8341 ggtctcgcaa cggttttcat cccggcgcgc aacgaacctg acctggatga cgtcccggcc
     8401 gaggtgctcg atgcgctgac ggtcaagccg atgaccgacg tcgccgagat cgtcgcgcag
     8461 gcgctggaac ctgcaacctt gacggtaacc gacgccgcct gacccgcagg ctcaggtcgc
     8521 gcctaggccc acgctcgtga cacgggcgtg ggcgtaaggc gttttcatgg cgtttttgat
     8581 ggcgttgaat gggaccatga gtttcaaacg ggagttagtg catcgcgtcc agcgaatggt
     8641 ggtcaacccg atcggccggc agttgccggt aaccatgctc gaaaccaccg gccgcaagtc
     8701 gggacgaccg cgccacacgg cggtgggcgg aaaggtcgtc gacaaccagt tctggatggt
     8761 ctccgaacac ggcgagcatt cggactacgt ctacaacatc aaggccaacc cggcggtgcg
     8821 agtccgcatc aacggccagt ggcgcaacgg aacggctcat ctgctgcccg acgacgaccc
     8881 acggcagcgg ctgcgcagcc tacctcggct caacagcgcg ggcgtggtca cgatgggcac
     8941 cgacctgctg accatccgcg tggacctggt ctgacgcaag gccgatggcc tacgacgaag
     9001 acctggccaa ccggatccgc gcactgctgg gctccgagcg gggcgtcgag gaaaagcgca
     9061 tgttcggcgg tctggcgtta ctgatcaacg gcaacatgtc cgtcgccgcc agcagcaagg
     9121 gtgggctgat ggtgcgcgtg ccgcccgagg acaccgacaa actgctggcg cgcgcccacg
     9181 tcagccccat ggtgatggcg ggccgggaaa cccgcggctg gttacgcgtt gacgccgacg
     9241 gcctgggaac caaacggcag cttcagagtt gggtgacccg cggcgtcggc tacgcgcgca
     9301 gcctgccgcc aaaataagtg ccccggtttg cgccgcgcgc caatgcgcgt atggccggtt
     9361 gaccccgtga cgcgtgggct tttgggctga tccgggccgg gtaggctgag gattccgaag
     9421 gcagaatgga gcagcagtca tggccagctc gaccacattc gtcatcgtcg gaggcggact
     9481 tgccggggcc aaagcggtag aagccctgcg cgacaacgac ttcgatggcc agatcattct
     9541 gttcgccgac gagggctacc cgccctacga gcggcccccg ctgtcgaaag agtatctggc
     9601 cggcaagaag acgctggccg acttcaccgt gcacaactcc gactggtacc gcgagcacaa
     9661 ggtcgacgtg aggctgggct cgcgggtgtc gtcgctggat cccgccgcgc acaccgtcgg
     9721 gctgcccgac ggcactacgg cggcctacca caagctgctg ctggcgacgg gatccgcgtc
     9781 gcggcggcca ccgatacccg gctcggatgc cgacggggtg tactacctgc gtacctacga
     9841 cgacgccgct gcgctgaatt ccgttctgac cgaagggtct tcgcttgcgg tggtgggcgc
     9901 cggctggatc ggcctggagg tggccgccgg tgcccgtcag cgcggagtcg gcgtcaccgt
     9961 cgtcgaggcg gccaaacagc cactggcggc cgcgctcgga gagacggtcg gcgaagtgtt
    10021 cgccacactg caccgcgatc acggcgtaga cctgaggc
//
LOCUS       DASSGQ010000353        22533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_238443,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000353 DASSGQ010000000
VERSION     DASSGQ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 22533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22533
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238443"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VFI55_15635"
     CDS             <1..381
                     /locus_tag="VFI55_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyacetophenone monooxygenase"
                     /protein_id="HET7075495.1"
                     /translation="ITVADVPNLFFLLGPNTGLGHNSVVFMIESQIHYVADAITTCDK
                     FGAQALAPTRAAQDRFNDELQHKLAGSVWNSGGCSSWYLDEHGRNTVLWGGYTWQYWR
                     ETRSVKPAEYQFFGVGSGVRASIA"
     gene            complement(336..1277)
                     /locus_tag="VFI55_15640"
     CDS             complement(336..1277)
                     /locus_tag="VFI55_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7075496.1"
                     /translation="MNSSNRADRQELIVVTGASTGMGAATARELARNGFHVLAGVRRD
                     ADADALAADGLEGLEPRILDITVESDVAAIAERVAHDPLHRPLRALVNNAGIAIHGPV
                     ETLPLTQWRRQFEVNLFGHIAMTQALLPALLRSSGTVVNISSIGGKFALPTYGAYAGS
                     KFALEAVSDSLRREVGDARIKVVVVEPGAVRTEMAERGIATAEGLKANLTAAQLERYG
                     GLIAAIAAQARSFNDIGVSAERAAQVIAKAATASRPRTRYTIGRDAAILALISRVVSD
                     RALDRIVRRNLRSFVTSSQSSSPHAIDARTPEPTPKN"
     gene            1382..1969
                     /locus_tag="VFI55_15645"
     CDS             1382..1969
                     /locus_tag="VFI55_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7075497.1"
                     /translation="MATRSESAAATRRSLLEAAGVLLDLGGVEAVTLREVGARSGVSR
                     SAAYRHFADKESLLSVLAANALSELGDALEVLAASDDSPETVLRSALLSLITIGRTRP
                     HLYRLMFTTPTGDPSAAVRAAERTQDLFLDIVARITGLRQAGRYGALLLTSAHGITGL
                     DLSGHLDLDKWHTNAEELVDTLISLLPKASHSVNG"
     gene            complement(1947..2498)
                     /locus_tag="VFI55_15650"
     CDS             complement(1947..2498)
                     /locus_tag="VFI55_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020724599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7075498.1"
                     /translation="MVRTPDSERRRQLLDALVEEFAAGGVGDRSLREVAGAVGTSHRM
                     LLHHFGSREDLLLAVVEEVERRQMGLLSEIPATAAEGFAAMWANLHRPELRRLERLFF
                     ECYARAAQGERPFDRMVPAAVDGWLSAVAAAADGPTDPAMARLGLAVTRGLLLDLVAT
                     NDDAGVDAAAQAFVEQLSRLPSD"
     gene            complement(2482..2928)
                     /locus_tag="VFI55_15655"
     CDS             complement(2482..2928)
                     /locus_tag="VFI55_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020724600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7075499.1"
                     /translation="MFAEDSVEIDAPPQLVWEVFTDVERWPQWTASVTSLVGLEGPGL
                     AVGRRFAIKQPGMQKLVWQVTEIDAGSSWTWVQRSPGVNVVARHYVSARPDGGTFVRQ
                     QLDQGGALGALVGRLMVKKTKRFLELEAQGLKARSEQLSRADGPHA"
     gene            3160..4134
                     /gene="nrdF"
                     /locus_tag="VFI55_15660"
     CDS             3160..4134
                     /gene="nrdF"
                     /locus_tag="VFI55_15660"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HET7075500.1"
                     /translation="MSEKMKLIDRVSAINWNRLQDDKDAEVWDRLTGNFWLPEKVPVS
                     NDLPSWGTLTPSEKQLTMRVFTGLTLLDTIQGTVGAVSLIPDALTPHEEAVYTNIAFM
                     ESVHAKSYSSIFSTLCSTAEIDDAFRWSEENVNLQRKAEIVMQYYRGDEPLKRKVAST
                     LLESFLFYSGFYLPMYWSSRAKLTNTADMIRLIIRDEAVHGYYIGYKFQRGLALADDA
                     KRAELKDYTYELLFELYDNEVEYTQDLYDEVGLTEDVKKFLRYNANKALMNLGYEALF
                     PRDETDVNPAILSALSPNADENHDFFSGSGSSYVIGKAVNTEDEDWDF"
     gene            complement(4241..4693)
                     /locus_tag="VFI55_15665"
     CDS             complement(4241..4693)
                     /locus_tag="VFI55_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7075501.1"
                     /translation="MTQRLETRLNPHAPIVLSLFRIFFGLLFLCHGTSLLFGRPADTV
                     AATGSLVWISGLIELTTGLLITLGLFTRPAAFIASGEMAVAFFTQHLPHGFWPISNGG
                     ELSVLYCWAFFLLVFTGPGAYALDTRLGRRGPAGVRNDLRRSPRRWRR"
     gene            4872..5192
                     /locus_tag="VFI55_15670"
     CDS             4872..5192
                     /locus_tag="VFI55_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HET7075502.1"
                     /translation="MSETKITVIYDNPSDPAAFEADYQSEQLEAARRIPGYLRLEASK
                     VWPKEDGSPTPAYRMIDLYYPDYAAVSAAVTTPEAGVFFAAMGRLATGGVRVLVSEVE
                     VPQH"
     gene            5807..6214
                     /locus_tag="VFI55_15675"
     CDS             5807..6214
                     /locus_tag="VFI55_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HET7075503.1"
                     /translation="MTSTQTSHKHAKFLRSVIRWLDVGYPQGVPGPDQVALFALLRST
                     PLTDEQLEEVVHNVIAKHRGEDEPVTHDEIAEFITKTTHYEPGRENIQRVAATLAAAG
                     WPLAGIDVSEVVPGDEDGEAAEQIASARASSEP"
     gene            complement(6203..7243)
                     /locus_tag="VFI55_15680"
     CDS             complement(6203..7243)
                     /locus_tag="VFI55_15680"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET7075504.1"
                     /translation="MSTVSAYAATSATEPLTKTTITRREPGPRDVAFDIKFAGICHSD
                     IHTVKAEWGQPNYPVVPGHEIAGVVTAVGREVTKFKVGDRVGVGCMVNSCGECSSCKA
                     GLEQYCKPGATFTYNSIDKDGTPTQGGYSQAIVVDENFVVRIPDALPLDKAAPLLCAG
                     ITLYSPLRHWKAGTNTRLAVIGLGGLGHMGVKLGAALGAEVTVLSQSLKKMEDGLRLG
                     ASHYYATADPDTFRELRGSFDLILNTVSANLNLGDYLNLLDVDGTLVELGIPEHPMEV
                     PAFPLALARRSLSGSNIGGIAETQEMLDFCAEHDVTPEIEVIGPDYINEAYERVLASD
                     VRYRFVIDTSGL"
     gene            complement(7368..8447)
                     /locus_tag="VFI55_15685"
     CDS             complement(7368..8447)
                     /locus_tag="VFI55_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7075505.1"
                     /translation="MVLAAAALAAVAAVACAGCGSSNPGPKASTPSLITPTTQIGGAG
                     VLGNDRHPDESCARDAAAADPGPPTRQAHNAAGVTPDAAQVPAEPQRIVVLSGDQLDA
                     VCALGLQSRVVGAALPDGSTSQPSYLGNAVHGLPGVGTRTNPDLGAIAAAHPDLILGS
                     QGLTPKLYPQLAAIAPTVFTAAPGAAWEDNLRGVGAATAREAAVDGLISAFAQRAGQI
                     GASHDAGHYQASIVQLTTDAIRVYGADNFPASVLAAVGVDRPAAQRFTDKAYIEFGAT
                     DADLAKNPDLSAVDADVVYVSCATPAAAERAAGVFDSGPWRKLSATHDNRVYVVNDEV
                     WQTGEGVIAARGIVDDLRLVNAPIN"
     gene            8655..10400
                     /gene="ctaD"
                     /locus_tag="VFI55_15690"
     CDS             8655..10400
                     /gene="ctaD"
                     /locus_tag="VFI55_15690"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HET7075506.1"
                     /translation="MTAEAPPLGELEAVRPYPARTGPKGNLVYKLITTTDHKMIGIMY
                     CVACICFFFIGGLLALLMRTELAAPGLQFLSNEQFNQLFTMHGTIMLLFYATPIVFGF
                     ANLVLPLQIGAPDVAFPRLNAFSFWLFLFGATMGAAGFITPGGAADFGWTAYTPLTDA
                     IHSPGPGGDLWIMGLIVAGLGTILGAVNMITTVVCMRAPGMTMFRMPIFTWNILVTSI
                     LVLVAFPLLTAALFGLAADRHLGAHVYDPANGGVLLWQHLFWFFGHPEVYIIALPFFG
                     IVSEIFPVFSRKPIFGYTTLVYATLSIAALSVAVWAHHMFATGAVLLPFFSFMTYLIA
                     VPTGIKFFNWVGTMWKGQLTFETPMLFSIGFMVTFLLGGLTGVLLASPPLDFHVTDSY
                     FVVAHFHYVLFGTIVFATFAGIYFWFPKMTGRLLDERLGKLHFWLTFIGFHTTFLVQH
                     WLGDEGMPRRYADYLPSDGFQGLNVVSTVGAFILGASMFPFAWNVFKSWRYGEIVTVD
                     DPWGYGNSLEWATSCPPPRHNFTELPRIRSERPAFELHYPHMVERLRAEAHVGRAHGP
                     KSGDVTRADDVNVRT"
     gene            10437..11672
                     /gene="serB"
                     /locus_tag="VFI55_15695"
     CDS             10437..11672
                     /gene="serB"
                     /locus_tag="VFI55_15695"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HET7075507.1"
                     /translation="MSTPSKVSVLITVTGVDQPGVTSALFEVLSRHGVELLNVEQVVI
                     RGRLILGVLLCCPPEVADGDELPKDVESAVRGLGLDVSIERSDDVPIIRQPPTHTIFV
                     LGRPVTARAFGAVAREAAALGVNIDVIRGVSDYPVTGLELRVSVPPGVNGKLKTALNR
                     VAADERVDVAVEDYGLVWRTKRLIVFDVDSTLVQGEAIEMLAARAGAQGQVAAITEAA
                     MRGELDFAESLQQRVATLAGLPATVIDEVADQLELMPGARTTLRTLRRLGFRCGVVSG
                     GFRRIIEPLAEELKLDYVAANDLEIIDGTLTGRVVGPIIDRPGKAKALREFAQQAGVP
                     LEQTVAVGDGANDIDMLATAGLGVAFNAKPALREVADASLSHPYLDTVLFLLGVTRGE
                     IEAADAIDGQLRRVEIPPD"
     gene            11757..12599
                     /locus_tag="VFI55_15700"
     CDS             11757..12599
                     /locus_tag="VFI55_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7075508.1"
                     /translation="MPETGSEPADPDLLIDFRNVSLRRGGNVLVGPVDWAVELDERWV
                     IIGPNGAGKTSLLRIAAAAEHPSSGIAFVLAERLGRVDVSELRARIGLSSSALAQRVP
                     GDEVVRDLVVSAGYAVLGRWRERYDEVDYRRAIDMLESLGAEHLADRTFGTLSEGERK
                     RVLIARALMTDPELLLLDEPAAGLDLGGREELVARLTDLAADPDAPAIVLVTHHVEEV
                     PPGFSHCMLLSEGRVVAAGLLPDVLTAENLSTAFGQSIALDVVDGRYFARRVRTRAAH
                     RRHS"
     gene            12647..13453
                     /locus_tag="VFI55_15705"
     CDS             12647..13453
                     /locus_tag="VFI55_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7075509.1"
                     /translation="MLVRDESPVGSGAGLAVFLMRRHSQMEFAAGVMVFPGGGVDDRD
                     RNADIAWAGPPPQWWAQRFGIEPDLAEALVCAAARETFEESGVLFAGPVGRSTSAPDG
                     AGGVVGDASVYRDYRHELASGALSFADFLRRENLVLRSDLLRPWANWVTPAAERTRRY
                     DTYFFVGALPEGQRADGENTESDRADWTTPRAAIDDFAAGRNFLLPPTWTQLDSLAGR
                     TVADVLAVERQIVTVQPHLEIQGDNWVFEFFDSDRYDEARKAGGSTGWPL"
     gene            13462..14238
                     /locus_tag="VFI55_15710"
     CDS             13462..14238
                     /locus_tag="VFI55_15710"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET7075510.1"
                     /translation="MREFVHVVVSDGSQDAGLGMLLVSRPPTNAMTRQVYREIVAAAG
                     ELGRRDDVAAVILFGGHEIFSAGDDMPELHTLTAPEAEIAARVRAEAVDAVAAIPKPT
                     VAAITGYALGAGLTLALAADWRVSGDNVKFGATEILAGLIPAGGGMARLTRAAGVSRA
                     KELVFSGRFFDAEEALALGLIDEMVAPDGVYDAAAAWARRFLDGPRQALAAAKAGINA
                     VFDEVAGLEPADRVAAEGRRYAEVFAAAHDGADKSGRHGR"
     gene            14250..15224
                     /locus_tag="VFI55_15715"
     CDS             14250..15224
                     /locus_tag="VFI55_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7075511.1"
                     /translation="MTMSSTDAVPNPRATAEQVEAARHDSKLAQVLYHDWEAESYDEK
                     WSISYDQRCIDYARDCFDGVVPDEVRHELPYDRALELGCGTGFFLLNLIQAGVARRGS
                     VTDLSPGMVKVATRNGKSLGLDIDGRIADAEGIPYDDNTFDLVVGHAVLHHIPDVELS
                     LREVIRVLKPGGRFVFAGEPTTVGNGYARALADLTWNVTIRAVRLPGLDSWRRPQAEL
                     DENSRAAALEWIVDLHTFEPADLERMATSAGAAEVQTATVEFTAAMLGWPLRTFEATV
                     PPGKLGWGWARFAFTSWKTLSWVDANIWRRVMPKGWFYNVMITGVKPS"
     gene            15221..16423
                     /locus_tag="VFI55_15720"
     CDS             15221..16423
                     /locus_tag="VFI55_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7075512.1"
                     /translation="MSSGLSFGTDDVGYLRSDSGAAALAAVAELDLSDASRIADVADV
                     RARFGDRAAVLIETTLLRRRAPEKLAQLSGGVSNWLFTDEALQQATAAPVALHRAGRL
                     AAGGPDVVVHDATCSIGTELAALRDSGVAAVGSDIDPVRLAMARHNLGAAADLCRADA
                     LYPVTRGTVVVVDPARRVGGRRRMRPEDYQPGLGRLIDTYRGRDLVVKCAPGIDFDQM
                     RRLGFDGEIEVTSYRGSVREACLWSAGLAQPGVGRRASLLDRGEQITDAEPDDCPVRP
                     VGRWIVDPDGAVVRAGLVRHYGARHGLWQLDPDIAYLSGDRLPPGVRGFEVVEQVVFE
                     ERRLRRALSALDCGALEILVRGVRVDPDALRRRLRPRGGSPLSVVITRIGAGAAGRAA
                     AFVCRPSR"
     gene            16494..17180
                     /locus_tag="VFI55_15725"
     CDS             16494..17180
                     /locus_tag="VFI55_15725"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008126 - acetylesterase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HET7075513.1"
                     /translation="MRYLIVAAVLTAAALLGWPAAAAPPPPCAGLGGTVDATELCHVH
                     ATGPTYMLNMAFPVDYPDEQALTDYITQNRDGFVNVAQTSGSRDQPYQMEATTEQHTA
                     GQPPHNTRSVVLKFFQDLGGAHPSNWFKAFNYNLGTKQPITFDNLFPPGTTPLDTIFP
                     IVQRDLERQNALGVAILPSTGLDPSHYQNFAITDDQLIFYFAPGEMLPALAGPVQAQV
                     PRNAIPPLAV"
     gene            complement(17186..17587)
                     /locus_tag="VFI55_15730"
     CDS             complement(17186..17587)
                     /locus_tag="VFI55_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6264 family protein"
                     /protein_id="HET7075514.1"
                     /translation="MPRPDPNHDPAPQNPASAHARRGHGSRPADVAATLVLLIVHGLL
                     FGATVVLLGLFVMGTDACGAVKCGDPAWVDRAMTLGIWAGAAVLIADFLVAGYLLLRR
                     RMAFFVPIIGCVAQVVLAILAAALESLAGPV"
     gene            complement(17574..18848)
                     /locus_tag="VFI55_15735"
     CDS             complement(17574..18848)
                     /locus_tag="VFI55_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7075515.1"
                     /translation="MLAAALTVGLGGCGNTDSWVDASPAQGWPAQYGDAANSSYTATS
                     GASKLSLQWTRSVKGSLAAGPALSARGYLALNAQTPAGCSLMQWENNDKGRQRWCVRL
                     FQGGGFAGPLFDGFDNLYVGQPGAFISFPVTQWTRWRKPVIGMPATPRFLGHGQLLVT
                     THLGQVLVFDAHRGEVVGSPLDLVEGIDPTDATRGLADCAPARQGCPLAAAPAFSTAN
                     GTVVLGVWQPGAPAAGLVGLKYHPGQTPLLVREWTSDAVGAGVIASPVLSADGSTVYV
                     DGRDQRLWALSAADGKVKWSVPLGFLAQTPPAVTPQGLIVSGGGPETRLAAFKDAGDH
                     ADPVWRRDDVTPLSSPSVAGVGVGYTVVSGPPADGEPGMSVLVFDPANGHTLNSYPLP
                     AATGYPIGVSVGNDRRVVAATSDGQVYSFAPA"
     gene            19095..19832
                     /locus_tag="VFI55_15740"
     CDS             19095..19832
                     /locus_tag="VFI55_15740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015288424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET7075516.1"
                     /translation="MTSMWGAPFHRRWRGSNLRDPRQARFLTLASLKWVLANRAYTPW
                     YLVRYWRLLKFKLANPHIITRGMVFLGKGVEIHATPELAEFEIGRWVHIGDKNTIRAH
                     EGSLRFGDKVVLGRDNVINCYLDIEFGDSALMADWCYVCDFDHRMDDINVPIKDQGII
                     KSPVRIGPDTWIGVKATVLRGTSIGRGCVVGSHAVVRGVIPDYSIAVGAPAKVVKNRR
                     LSWETSAAQRAELAAALSDIERKKAAR"
     gene            complement(19837..20382)
                     /locus_tag="VFI55_15745"
     CDS             complement(19837..20382)
                     /locus_tag="VFI55_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016719280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HET7075517.1"
                     /translation="MAHSIVRTLLVAGAAAGLAAGTGACSCSIGSSSHAVSKTDIANQ
                     ITSKLTDAAGNKPDSVTCPSDLPAKVGAQLNCTMKVKDQTYTVNVTVTNVNGDDVKFD
                     LAETVDKNQVANVISYELTQQLGRKPDSVTCPDNLKGVQGATLRCELTDSDQKYGVAV
                     IVRNVDAGNVNFDFKVDDHPE"
     gene            complement(20490..21817)
                     /locus_tag="VFI55_15750"
                     /pseudo
     CDS             complement(20490..21817)
                     /locus_tag="VFI55_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978299.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
     gene            complement(21832..22311)
                     /locus_tag="VFI55_15755"
     CDS             complement(21832..22311)
                     /locus_tag="VFI55_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HET7075518.1"
                     /translation="MKKSTLAATTLAVATAAGTGSIASPKRVPAWYSRLRKPSYQPPG
                     AAFPVVWTTLYGNIAATSAVAIDRLRAAGQHDKARRYAAALSLNLLLNAGWTWLFFHY
                     HKLGASALGAAALTASSADLARRAAEADRRARSALSAYPLWGAFATVLATHVWRLNR"
BASE COUNT         3642 a         7477 c         7623 g         3791 t
ORIGIN      
        1 atcaccgtcg ccgacgtgcc caacctgttc ttcctgctgg ggcccaacac cggactcggg
       61 cacaactccg tggtgttcat gatcgaatcg cagatccact acgtcgccga cgccatcacg
      121 acctgcgaca agtttggggc gcaggcgctg gccccgacgc gcgccgcgca ggacaggttc
      181 aacgatgagc tgcaacacaa gctcgccggc tcggtgtgga acagcggcgg ctgcagcagc
      241 tggtatctcg acgagcacgg ccggaacacc gtgctctggg gcggctacac ctggcagtac
      301 tggcgggaga cccgctcggt caaacccgcc gagtatcagt tcttcggggt tggttcggga
      361 gttcgcgcgt caatcgcgtg aggtgagctc gactgcgagc ttgtgacaaa ggaccgaagg
      421 ttgcggcgca ctatgcggtc caaggcccgg tcggagacga cgcggctgat cagcgcgagg
      481 atcgcagcgt cgcgtccgat ggtgtagcga gtgcggggac gcgacgcggt cgccgccttg
      541 gcgatcactt gtgcggcgcg ctcggccgaa acgccgatgt cgttgaacga tcgggcttgt
      601 gccgcgatgg ctgcgatgag accgccgtag cgttcgagtt gggcagcggt caggttggcc
      661 ttcagcccct ccgcggtagc gattccgcgc tccgccatct cggtcctgac tgcgccgggt
      721 tcgacgacga cgaccttgat ccgggcatca ccgacctcac ggcgtagcga gtcgctgacc
      781 gcctcgaggg cgaacttcga accggcgtat gcgccgtaag ttggcagggc gaacttgccg
      841 ccgatggaac tgatgttcac cacggtgccc gagcttctca gcagggccgg caacagcgcc
      901 tgggtcatcg cgatgtgacc gaacagattg acctcgaact gccggcgcca ttgcgtcagc
      961 ggcagcgttt cgaccggccc atggatggcg atacccgcgt tgttgaccag cgcgcgaagg
     1021 gggcgatgca gcggatcgtg cgcgacacgt tcggcgatcg cggccacgtc cgattcgacg
     1081 gtgatgtcga ggatgcgcgg ttctagcccc tcaagcccgt cggccgctag cgcgtcggcg
     1141 tcggcgtcgc ggcgtacacc ggcgagcacg tgaaaaccgt tgcgggccag ttctcgagcc
     1201 gtggctgcgc ccatacctgt cgaggcgccg gtcacgacga tcagttcttg acggtcggcg
     1261 cgattcgagg aattcatggc ggtctccgtc cggtggcggt cagcatttga gttgacgctg
     1321 tcatctcaaa ctaacacttg agttgacgtt gtcaactcaa gcaccggctt atgctcttcg
     1381 gatggcgacg cgatccgaga gtgctgcggc cacccgccgt tcgctgctcg aggcggcggg
     1441 ggtcctactc gacctcggcg gagtcgaggc cgtgacgctg cgcgaggtgg gtgctcgcag
     1501 cggcgtatcg cgctcggcgg cgtatcgaca cttcgcggac aaggaatctc tgctcagcgt
     1561 cctggccgcg aacgcgttga gcgaattggg tgatgcgctc gaggtgttgg ccgccagcga
     1621 cgactcgccc gagacggtat tgcgatccgc tctgctttcg ctgatcacca tcggccgcac
     1681 ccggccccac ctgtaccgcc tgatgttcac caccccgacg ggtgatccga gcgccgcggt
     1741 ccgggcggcc gaacgcacac aggacctgtt tctcgacatc gtggctcgca tcaccggcct
     1801 gcggcaggcg ggacgctacg gagcgctcct gttgaccagc gcccacggca tcacgggctt
     1861 ggatttgagc ggccacctgg acttggacaa gtggcacacc aacgccgagg aactcgtcga
     1921 cacgctcatc tcgctgctgc cgaaagctag tcactcggta aacggctgag ctgctcgaca
     1981 aaggcctggg cggcggcgtc cacgccggca tcatcgttgg tggccaccag atccagcagc
     2041 agcccccggg tgacggccag cccgagccgg gccatcgcag ggtctgtcgg gccgtccgcc
     2101 gcggccgcga ccgcgctcag ccagccgtcg acggcggcag ggaccatccg gtcgaacggc
     2161 ctctcgccct gggcggcgcg ggcgtaacac tcgaagaata ggcgttccag ccggcgtagc
     2221 tcggggcgat gcaggttggc ccacatggca gcgaagcctt cggcggccgt ggccggtatt
     2281 tcggacagta ggcccatctg gcggcgctcg acctcctcga cgacagccag gagcagatcc
     2341 tctcgagacc cgaagtggtg cagcaacatt cgatggctgg tgccgaccgc gccggctacc
     2401 tcgcgcagtg agcggtcgcc tacgccgccc gccgcgaact cctcaacgag cgcgtcgagc
     2461 agctgccggc gacgctcgga gtcaggcgtg cggaccatcg gcgcggctga gctgctccga
     2521 ccgggccttg aggccctggg cctccagttc gaggaagcgt ttggtcttct tgaccatcaa
     2581 ccgcccgacc agcgcgccca gggccccgcc ctggtcgagt tgctggcgca cgaaggtgcc
     2641 gccgtcgggt cgggcgctca catagtggcg agcgacgacg ttcacgccgg gggagcgttg
     2701 cacccacgtc caggacgagc ccgcgtcgat ctcggtgacc tgccagacca acttctgcat
     2761 gccgggctgc ttgatcgcga accgtctgcc gacggcgagg ccgggtcctt ccagcccgac
     2821 cagagaggtc accgaggcgg tccactgggg ccagcgctcg acgtcggtga agacctccca
     2881 taccagttgc ggcggcgcat cgatttcgac agagtcttcg gcaaacatgt accaaatggt
     2941 acatatattg tccggttttg cctaggcctt cacctggcgg gggatgccga tcactccgtt
     3001 gggccggcct gcgctgacgc gagccgtgga aatggcgtgg cgcttggccg cacgacacgg
     3061 caaacacaag ctctggtgtt gcaccggctt gtcatacccc aggggtagtg tcggaagtcc
     3121 aagtaccgca ggcaaaacgt ctggtgaagt ggggttctgg tgagcgaaaa gatgaagttg
     3181 attgaccgcg tctcggcgat caactggaac cggctgcaag acgacaagga cgccgaggtc
     3241 tgggaccggc tgacgggcaa tttctggctg ccggagaagg tgccggtgtc caacgacctc
     3301 ccgtcgtggg gcacgctgac gcccagcgag aaacagctga cgatgcgggt gttcaccggg
     3361 ctgacgctgc tggacaccat tcagggcacg gtcggagcgg tcagcctgat tcccgacgcg
     3421 ctgaccccgc acgaagaggc cgtctacacc aacatcgcct tcatggagtc ggtgcacgcc
     3481 aagagctaca gctcgatctt ctccacgctg tgctcgaccg ccgagatcga cgacgccttc
     3541 cgctggtcgg aggagaacgt caacctccag cgcaaagccg agatcgtgat gcagtactac
     3601 cgcggcgacg agcccctgaa gcgcaaagtg gcctccacgc tgctggaaag cttcctcttc
     3661 tactccgggt tctacctgcc catgtactgg tcgagccggg ccaagctgac caacaccgcc
     3721 gacatgatca ggctgatcat ccgcgacgag gccgtgcacg gctactacat cggctacaag
     3781 ttccagcgtg gcctggcgct ggccgacgac gccaagcgcg ccgagctgaa ggactacacc
     3841 tacgaactgc ttttcgagct gtacgacaac gaggtggagt acacccagga cctctacgac
     3901 gaggtcgggc tgaccgagga cgtcaagaag ttcttgcgct acaacgccaa caaggcgctg
     3961 atgaacctgg gctacgaggc actgttcccg cgcgacgaga ccgacgtcaa cccggcgatc
     4021 ctgtcggcgt tgtcgcccaa cgccgacgag aaccacgact tcttctccgg ctcgggctcg
     4081 tcgtacgtca tcggcaaggc cgtcaatacc gaggacgagg actgggattt ctgaatctgc
     4141 tggtcagatg ccctgtcacg cccgaatact gccgtgccgc aagcgcttca ggagttccca
     4201 cataacctgg gtgccgacgt gacgtctggg ttctacgttg ctatcgacgc cagcgccggg
     4261 gcgatctacg caaatcgttt cttactcccg cggggccacg tcgccccaac ctggtgtcca
     4321 gtgcgtaggc accgggcccg gtgaacacca gcagaaagaa cgcccagcag tacaggaccg
     4381 agagttcacc gccattgctg atcggccaga acccgtgcgg aagatgttgg gtgaagaacg
     4441 cgacagccat ctcgcccgag gcgatgaaag ccgctggacg cgtgaacagt ccgagagtga
     4501 tcaagaggcc ggtggtcaat tcgatcaacc cggagatcca gacgagcgag ccggtggccg
     4561 cgacggtatc agccggccgg ccgaacagca gggacgtgcc gtggcacagg aacaggagtc
     4621 cgaagaagat ccggaagagg ctcaagacta tcggcgcatg cgggttgagt cgggtttcca
     4681 gccgttgggt catggggctg acgatacgca agatagcgcc ccgggaagcg agcaatcttt
     4741 tgccaggtca ggtacaccgg ccgggctcga aagtctggcc cccggaacct cctgcgccca
     4801 agaccaggct ccttcatcga aggtagggtc cagcacaggc tcgacttcat cgacgacgcg
     4861 agggaaatcc attgtccgaa accaagatca cggtcatcta tgacaacccc tccgacccgg
     4921 ccgccttcga agccgactat cagtcggagc aattggaagc cgcgcggcga ataccggggt
     4981 atctccggct cgaggcgtcg aaggtctggc cgaaagaaga tgggagtcct acgcctgctt
     5041 accgcatgat cgacctgtac tacccggatt acgctgccgt cagtgcggcg gtgactacac
     5101 cggaagccgg cgtgttcttt gccgctatgg ggcgactcgc caccggcggc gtacgtgtac
     5161 tcgtctccga ggtcgaggtg ccgcagcact gatcgcgagc cgcctaacac caacggcgcc
     5221 aaagcggctg caccacgcgt gatgctcggg agcggtctgc gtcagtggta gcacgatctc
     5281 gcgcgcggca ttatgggtaa ccgtgccgca atgggaacca ctcgagccga atccaaacga
     5341 tccgctgcgc taccgcatgg tcagcgatgg gcatgactac cgcattctcg atgaccccga
     5401 aacggggcga tcgggtcgcc cgtggattct ggcgatcgga cagctccacc aagatggctt
     5461 gcacgtactc gtccatctaa cggaccacgc caacgtcgac gatgcgaagc gagccgccga
     5521 gatatggagg ggcccaccgg cagcgcgatc aggccgccac caccgagaag agtaggggcg
     5581 gcccgaccag cgagtcggac gccccattcc atgtcgcacg atccgacgag tgcgtcgttt
     5641 cagcgctgcc aactgggggc cggattagaa ccacgacttc ttctccggtt ccggatcgtc
     5701 gtcagtcacc gagaacgaag actgggaatt ctaagtggcg atgaacggcg tgtgaattgt
     5761 cgcagggtgt aacgcgacga tgacgcccag ccatgacact aatggggtga cctcaaccca
     5821 gacttcgcac aagcacgcca aatttctgcg ttcggtgatc cggtggctgg acgtgggcta
     5881 cccccaaggc gtccctgggc cggaccaagt cgcactgttc gccctgttgc gcagcacccc
     5941 gttgaccgac gagcaactcg aagaagtcgt gcacaacgtc atcgcgaagc accgaggcga
     6001 agacgagccg gtcacccatg acgagatcgc cgagttcatc accaagacga cccactacga
     6061 gcccggccgg gaaaacatcc agcgcgtagc cgccacgctg gcggccgcgg ggtggccgct
     6121 ggccggtatc gacgtcagcg aagtagtgcc cggtgacgag gacggggaag cggccgagca
     6181 gatcgcttcg gcgcgcgctt cgtcagagcc ctgacgtgtc gatcacgaag cggtagcgca
     6241 cgtcgctggc gagcacgcgc tcgtacgctt cgttgatgta gtccggcccg atgacctcga
     6301 tctcgggcgt gacgtcgtgc tcggcgcaga aatccagcat ctcctgcgtc tcggcgatgc
     6361 cgccgatgtt ggagccggaa aggctgcgcc gggccagtgc cagcgggaat gccggcacct
     6421 ccatggggtg ctcagggatc ccgagttcga ccagcgtgcc gtcgacgtcc agcaggttga
     6481 ggtagtcgcc gagattcagg ttcgccgaaa ccgtattgag aatcaggtcg aagctgccgc
     6541 gcagctcgcg gaaggtgtcc gggtcggccg tcgcgtagta gtggctggca cccaggcgca
     6601 gtccgtcctc cattttcttc agcgactgcg acagcaccgt cacctcggcg cccagcgccg
     6661 cgcccagctt gacgcccatg tgcccgaggc cgcccaggcc gatcaccgcg agccgcgtgt
     6721 tcgtccccgc cttccagtgc cgcagcggcg agtacagcgt gatgccggcg cacagtagcg
     6781 gcgccgcctt gtccagtggc agggcgtcgg gtatgcggac gacgaagttc tcgtcgacga
     6841 cgatcgcctg gctgtagccg ccctgggttg gcgtgccgtc cttgtcgatg gagttgtagg
     6901 tgaaggtggc gccgggtttg cagtactgct ccagcccggc cttgcagctg ctgcattcgc
     6961 cgcaggagtt gaccatgcag cccaccccga cgcggtcgcc gaccttgaat ttggtgactt
     7021 cgcggcccac cgcggtcacc acgccggcga tctcgtggcc ggggaccacg gggtaattcg
     7081 gttggcccca ctcggctttg acggtgtgga tgtccgagtg gcagatcccc gcgaatttga
     7141 tgtcgaacgc cacgtcgcgc gggccgggct cgcgacgggt gatggtggtc ttggtcaggg
     7201 gttcggtggc cgatgtggcg gcgtaggcgg aaacggtgct catcaagtaa tcctcttcga
     7261 tctagtcagt ccccgaaaag cttagctaag gtaatgttct caccctaacc gcggggtggg
     7321 gcccggacat cagcttagga cgatgtacgc ccatcggccg ccgggcgtca gttgatcggg
     7381 gcgttgacca accgcaggtc gtcgacgatg ccgcgggccg cgatcacgcc ctcgccggtc
     7441 tgccatacct cgtcgttgac gacgtaaaca cggttgtcgt gggtggccga aagcttgcgc
     7501 cacgggccgc tgtcgaagac gccggcggcg cgctcggcgg cggccggcgt ggcgcacgac
     7561 acatagacga cgtcggcgtc gacggccgag agatccggat tcttcgcgag gtcggcatcg
     7621 gtggcgccga attctatgta cgccttgtcg gtgaaccgct gtgccgccgg ccggtccacc
     7681 cccaccgccg ccagcacact ggccgggaag ttgtcggccc cgtacacccg aatggcgtcg
     7741 gtggtcagct gcacgatcga cgcctggtag tgacccgcgt cgtggctggc cccgatctgg
     7801 ccggcccgtt gcgcgaaggc gctgatcaac ccgtcgacag cggcttcccg tgcggtcgcg
     7861 gcaccgacgc cacgcaggtt gtcctcccag gccgctccgg gtgccgcggt gaacactgtc
     7921 ggcgcgatcg cggccagctg cgggtacaac ttaggcgtca acccctgcga tccgaggatc
     7981 agatcggggt gagccgccgc gatggcgccc aggtcggggt tggtgcgggt gccgacgccg
     8041 ggtaggccgt gcacggcatt gcccaggtag gacggctggc tcgtggagcc gtccggcaac
     8101 gcggcgccga ccacccgcga ttgcaggccc agcgcgcaga cggcgtcgag ctggtcaccg
     8161 gagagcacca cgatgcgctg cggctccgcg gggacctgcg cggcgtccgg ggtgactccc
     8221 gccgcgttgt gggcctgtcg tgtcggcggt cccggatcgg ccgcggcggc gtcgcgcgcg
     8281 cacgactcgt ccggatggcg atcgtttccc aatacccccg cgccgccgat ctgggtggtg
     8341 ggggtgatca gcgacggagt cgacgctttg ggacccggat tggatgaccc gcagccggcg
     8401 caggccaccg ccgccaccgc ggccagcgcg gcggcggcca gcaccagccg cgtgggtcgg
     8461 atctcgccgg attgcacgag tccgacgcta acatccgccc agcttggacc cgtccggcga
     8521 aacgatccac cacctccggt gaaccattta cgacagtttg taggaccaga cctaccgtcg
     8581 ccttgatcgg gagctaagat tcctgtcatt actgggttct ggcccctgtc cgacacggat
     8641 gtttggagga gctgttgaca gccgaagccc ccccgttggg agaactcgag gccgtccgtc
     8701 catacccggc ccggaccggt cccaaaggga acctcgtcta caagctgatc accaccaccg
     8761 atcacaaaat gatcggcatc atgtactgcg tcgcgtgcat ctgcttcttc ttcatcggcg
     8821 gcctgctggc gctgttgatg cgtaccgagc tggccgcgcc gggtctgcag ttcctgtcga
     8881 atgagcagtt caaccagctg ttcaccatgc acggcacgat catgttgctg ttctacgcga
     8941 ccccgatcgt gttcggcttt gccaacctgg tgctgccgct gcagatcggc gcacccgacg
     9001 tggcgttccc ccgcctcaac gcgttctcgt tctggctgtt cctgttcggc gccacgatgg
     9061 gcgcggccgg cttcatcacg cccggcgggg ccgccgactt cggctggacg gcctataccc
     9121 cgttgaccga tgccatccac tcgcccggcc cgggcggtga cctgtggatc atgggtctga
     9181 tcgtcgccgg tctgggcacc atcctgggcg cggtcaacat gatcaccacc gtggtgtgca
     9241 tgcgcgcccc cggcatgacg atgttccgga tgccgatctt cacctggaac atcctggtga
     9301 cctcgatcct ggtgcttgtc gcgttcccgc tgctcaccgc ggccctgttc gggctggccg
     9361 ccgaccgcca cctcggtgcc cacgtctacg acccggccaa cggcggagtc ctgctgtggc
     9421 agcacctgtt ctggttcttc ggccatcccg aggtctacat catcgcgctg ccgttcttcg
     9481 gcatcgtctc ggagatcttc ccggtctttt cccgcaagcc gatcttcggt tacaccacgc
     9541 tggtctacgc gacgctgtcg atcgccgcgc tgtcggtcgc ggtgtgggcg caccacatgt
     9601 tcgccaccgg agccgttctg ctgccgttct tttcgttcat gacgtatctg atcgcggtgc
     9661 cgacggggat caagttcttc aactgggtcg gcacgatgtg gaagggccag ttgacgtttg
     9721 agacgccgat gctcttctcg atcggcttca tggtcacctt cctgctgggc ggtctgaccg
     9781 gtgtgctgct ggccagcccg ccgctggact tccacgtcac cgacagttat ttcgtggtcg
     9841 cgcacttcca ctacgtgctc ttcggcacca tcgttttcgc caccttcgcc ggcatctatt
     9901 tttggttccc gaagatgacc ggccgcctgc tcgacgagcg actgggcaag ctgcacttct
     9961 ggctgacgtt catcggcttt cacacgacgt tcctggtgca gcactggttg ggcgacgaag
    10021 gtatgccgcg ccgctacgcc gactacctgc ccagcgacgg cttccagggc ctgaacgtcg
    10081 tctccactgt gggggcattc atcttgggtg catcgatgtt cccgttcgcg tggaacgtgt
    10141 tcaagagctg gcgctacggc gagatcgtga cggtcgacga cccgtggggc tacggcaact
    10201 cgctggaatg ggcgaccagt tgcccgccgc cgcggcacaa cttcaccgag ctgccccgga
    10261 tccgttcgga gcggccggca ttcgagttgc actacccgca catggtggaa cggcttcggg
    10321 ccgaggcgca cgtggggcgg gcgcacgggc ctaaaagcgg cgatgtcacc agagccgacg
    10381 acgtcaacgt tcgcacctag cgggcgaaag ccgccccaaa cgaggctaag tcgcgggtga
    10441 gcacaccatc aaaggtgtcg gtactgatca cagtcaccgg cgtcgatcag ccgggcgtga
    10501 cgtctgccct gttcgaggtg ctctcccggc atggggtaga gctgctcaac gtcgaacagg
    10561 tggtgatccg gggccgcctg atcctgggtg tgttgctgtg ctgcccgccg gaagtggccg
    10621 acggcgacga gctgcccaag gacgtcgaat ccgccgtccg cggactgggt ctcgacgtca
    10681 gcatcgagcg cagcgacgac gtgccgatca tccggcaacc gccgacccac accattttcg
    10741 tgctgggccg gccggtcacg gcccgggcgt tcggagcggt ggcccgggag gcggcggcgc
    10801 tgggcgtcaa catcgacgtc attcgcggtg tctccgacta cccggtgacc ggcctggagt
    10861 tgcgggtctc ggtgccgccg ggtgtcaacg gcaagttgaa aaccgcgctg aaccgggtgg
    10921 ccgccgacga gcgtgtcgac gtggccgtcg aggactacgg cctggtctgg cgcaccaaac
    10981 ggctcatcgt gttcgacgtc gactcgacgc tggttcaggg tgaggccatc gagatgctgg
    11041 ccgcccgtgc cggagcccag gggcaggttg ccgcgatcac cgaggccgcg atgcgcggcg
    11101 agctggattt cgcggagtcc cttcagcaac gggtcgcgac cctggcgggc ctgcccgcca
    11161 cggtgatcga cgaggtcgcc gatcagctgg agctgatgcc cggcgcgcgg accacgcttc
    11221 gcacgttgcg gcgcctgggt tttcgctgcg gagtggtctc cggcgggttt cgccggatca
    11281 tcgaaccgtt ggccgaggaa ctgaagctgg actacgtggc cgccaacgac ctggagatca
    11341 tcgacggcac cctcaccggc cgggtggtcg ggccgatcat cgatcggccc ggcaaggcca
    11401 aggcgctgcg cgagttcgca cagcaggccg gcgtgccgtt ggagcagacg gtcgccgtcg
    11461 gcgacggcgc caacgacatc gacatgctgg ccacggccgg actgggtgtg gcgttcaacg
    11521 ccaagccggc gctgcgtgag gtcgccgatg cgtcgttgag ccacccgtac ctcgacacgg
    11581 tgctgtttct cctgggtgtg acgcgcggcg agatcgaggc cgccgacgcg atcgacggtc
    11641 agctgcgacg ggtcgaaatc cccccggact agtcgcgcgc gcgggcggcc ccatccggtt
    11701 gcggggatgc acgacccggc aggtatccga gcgtcgcggt acggcacgat ggtcgggtgc
    11761 ccgaaaccgg aagcgagccg gccgaccccg atctgctgat cgacttcaga aacgtgtccc
    11821 tgcgacgcgg cgggaacgtc ctggtcgggc ccgtggattg ggcggtggaa ctcgacgagc
    11881 gctgggtgat catcgggccc aacggcgccg gcaagacatc gctgctgcgc atcgccgcgg
    11941 cggccgagca cccgtcgtcg ggcatcgcct tcgtgctcgc cgagcggctg ggccgggtcg
    12001 acgtttcgga gttgcgtgcg cggatcgggc tcagttcgtc ggctctggcg cagcgggtgc
    12061 ccggcgatga agtggtgcgc gatctcgtcg tctcggcggg ctatgcggtg ctgggtcggt
    12121 ggcgggaacg ctacgacgaa gtcgactacc ggcgggcaat cgacatgctg gaaagcctcg
    12181 gcgccgagca cctcgcggac cgcaccttcg ggacgttgtc ggagggggag cgcaagcggg
    12241 tgctgatcgc ccgcgcattg atgaccgacc ccgaactgct gctgctcgac gaacccgccg
    12301 ccggcctgga cctgggcggc cgcgaggagt tggtcgcccg gctgaccgac ctggccgcgg
    12361 accccgacgc ccccgcgatc gtgctggtca cccaccacgt ggaggaggtc ccccccggct
    12421 tcagccactg catgctgttg tcggagggcc gggtggtcgc cgccggtctg ctgcccgacg
    12481 tgctgaccgc cgagaacctg tccaccgcat ttggccagtc catcgccctg gacgtcgtcg
    12541 acgggcgcta tttcgcgcgg cgcgtgcgca cccgcgcggc tcaccggagg cattcgtgac
    12601 ggagtcgagc gagccgccgc tgacaccgcg gccggccgcg accgtgatgc tggtccgcga
    12661 cgaaagtcca gtcggctcgg gggccggctt ggccgtcttc ctcatgcggc ggcactcgca
    12721 gatggagttc gccgcgggag tgatggtgtt tcccggcggc ggcgtcgacg accgcgaccg
    12781 caacgcggac atcgcgtggg ccgggccacc gccgcagtgg tgggcgcagc ggttcggcat
    12841 cgaacccgac ctggccgagg cgctggtctg cgcggccgcc cgggagacct tcgaggaatc
    12901 gggcgtgctg ttcgccggac cggtcggccg gtcaacttcg gcaccggacg gcgccggcgg
    12961 ggtcgtcggc gatgcctcgg tgtatcgcga ctaccgtcac gagctggcct cgggcgcact
    13021 gtctttcgcg gacttcctgc gccgcgaaaa cctggtgctg cgatccgatc tgttgcggcc
    13081 gtgggccaac tgggtcactc ccgcggccga gcgcacccgc cgttacgaca cctatttctt
    13141 tgtgggagcg ctgccggagg gtcagcgggc cgacggtgag aacaccgaat ccgaccgcgc
    13201 cgactggacg acgccgcggg ccgcgatcga cgacttcgcc gccggtcgca acttcctgct
    13261 gccgcccacc tggacacagc tcgactcgct ggccggtcgc accgtcgccg atgtgttggc
    13321 cgtcgagcgc cagatcgtca cggtgcagcc acatctcgag atccagggcg ataactgggt
    13381 gttcgaattc ttcgattccg accgctacga cgaggcacgg aaagcgggcg gatccacggg
    13441 ttggccgctg tgacggctcc ggtgcgtgag ttcgtccacg tggtggtcag cgacggctcg
    13501 caggacgccg gcctgggcat gctgctcgtg tcccggccgc cgaccaacgc gatgacccgg
    13561 caggtctacc gggagatcgt tgccgcggcc ggggagctgg gacggcggga cgacgttgcg
    13621 gcggtcatcc tgttcggcgg tcacgagatt ttctccgccg gcgacgacat gcccgagctg
    13681 cacacgctga cggcgccgga ggccgagatc gcggcccgcg tccgtgccga ggccgtcgac
    13741 gccgtcgcgg cgatccccaa gccgacggtg gccgcgatca ccggatacgc gctcggcgcc
    13801 ggcctcacgc tggccctggc cgccgactgg cgggtcagcg gcgacaacgt gaagttcggc
    13861 gcgaccgaga tcctggccgg cctgatcccc gccggcggcg gaatggcccg cctgacccgc
    13921 gccgccggcg tgagtcgcgc caaggagctg gtattcagcg ggcgtttctt cgacgccgag
    13981 gaggctctgg ccttgggctt gattgatgag atggtggccc ccgacggcgt gtacgacgcc
    14041 gccgcggcgt gggcacgccg ttttctcgac ggcccgcggc aggcactggc cgccgccaaa
    14101 gccggcatca atgccgtctt tgacgaagtc gcgggcctgg aaccggccga tcgcgtcgcg
    14161 gccgagggcc gccgctatgc ggaggtcttc gccgctgctc acgacggtgc cgacaagtct
    14221 ggccggcacg gtcgttaggc ttgccctcca tgacgatgag ttcgactgac gccgtcccca
    14281 acccgcgtgc caccgccgag caggtggaag ccgcacggca cgacagcaag ctggcccagg
    14341 tgctctacca cgactgggaa gccgagagtt acgacgaaaa atggtcgatc tcctacgacc
    14401 agcgctgcat cgactacgcc cgggattgct tcgacggcgt cgtgcccgac gaggtccggc
    14461 acgagctgcc ctacgaccgt gccctggagc tgggctgtgg gaccgggttc ttcctgctca
    14521 atctgatcca ggcgggggtc gcccggcgcg gttcggtcac cgacctttcg cccggcatgg
    14581 tcaaggtcgc cactcgcaac gggaagtcgc tggggctgga catcgacggc cggatcgcgg
    14641 acgccgaggg catcccgtat gacgacaaca cctttgatct cgtggtcggg cacgccgtgc
    14701 tgcatcacat tcccgacgtc gaactgtcgc tgcgcgaggt gatccgagtc ctcaagcccg
    14761 gcggccggtt cgtgttcgcc ggtgagccga caaccgtcgg caacggatat gcccgtgcgc
    14821 tggcggacct cacctggaac gtcaccatcc gtgcggtcag gctgcccgga ctcgacagct
    14881 ggcgccgtcc gcaggctgag ctcgacgaga actcgcgtgc ggcggccctg gagtggatcg
    14941 tcgacctgca cacgttcgaa cccgcggacc tggagcgcat ggccaccagc gccggcgccg
    15001 ccgaggtcca gaccgccacc gtggagttca ccgccgccat gctgggctgg ccgctgcgca
    15061 cgttcgaggc gacggtgccg ccgggcaaac tgggctgggg ctgggcgagg ttcgccttca
    15121 ccagttggaa gacgctgagc tgggtggacg ccaacatctg gcgtcgcgtg atgcccaagg
    15181 gctggttcta caacgtgatg atcaccgggg tcaagccgtc ctgagcagtg gcctcagctt
    15241 cggtacggac gacgtcggtt acctacggtc ggactcgggt gccgcagcgc tggccgcggt
    15301 cgccgagctc gacctgtcag acgccagccg gatcgccgac gtcgccgatg tgcgtgcccg
    15361 gttcggcgac cgggcggcgg tgctgatcga gacgacgctg ctgcgccgta gggctcccga
    15421 gaagctggcg cagctaagtg gtggggtgtc gaactggctg ttcaccgacg aggctctgca
    15481 gcaggccacc gcggcgccgg tggccctgca ccgggccggg cggctggccg cgggtggccc
    15541 agacgtcgtg gtccatgacg cgacctgttc gatcggcacc gaactggccg cgctgcgcga
    15601 ctcgggtgtc gcggcggtgg gcagcgacat cgacccggtc cggctggcga tggcccgcca
    15661 caacctgggg gcggctgccg acctctgccg cgccgatgcg ctgtatcccg ttacccgcgg
    15721 cacggtggtc gtggtcgacc cggcgcgacg tgttggcggg cggcggcgca tgcgccccga
    15781 ggactaccag ccgggcctgg gccggctgat cgacacctac cgggggcggg atctcgtcgt
    15841 aaagtgcgct cccggaatag atttcgacca gatgcgacgg ctcggtttcg acggcgaaat
    15901 cgaggtcacg tcgtatcgcg gttcggtccg ggaggcgtgc ctgtggtcgg cgggactggc
    15961 gcagcccggt gtcggtcgcc gggcgagcct ccttgatcgc ggcgaacaga ttaccgacgc
    16021 cgagcccgac gactgcccgg tgcggccggt cgggcgatgg atcgtcgacc ccgacggcgc
    16081 ggtggtccgg gccggcctgg tgcgccacta cggcgcgcgg cacggactgt ggcaactcga
    16141 ccccgacatc gcctacctgt ccggggaccg gctgccgccg ggtgtccggg gcttcgaggt
    16201 ggtcgagcag gtggtgttcg aggagcgccg gctgcggcgg gcgttgtcgg cgctcgattg
    16261 cggtgcgctg gagatactgg tccgcggtgt gcgggtcgat cccgacgcgc tgcggcgccg
    16321 gctgcggccg cgtggcggca gtccactctc ggtggtcatc acccgcatcg gcgccggggc
    16381 cgccggccgc gcggcggcct tcgtttgccg tccgtctcga tagtgcgcgg tacgctggct
    16441 gccgtcactc ttcccgccgc ccagcggttt tagtccgcga ggagatttcg acgatgcgtt
    16501 atctgatagt ggccgcggtt ttgactgccg cggccttgct gggctggccg gccgccgcgg
    16561 cgccgccgcc gccgtgtgcc ggcttgggcg gcaccgtcga tgcgacggag ctgtgtcatg
    16621 tgcatgccac cggccccacg tacatgctca acatggcgtt ccccgtcgac taccccgacg
    16681 agcaggcgtt gaccgactac atcacgcaaa accgcgacgg gttcgtcaac gtcgcgcaga
    16741 cttccggaag ccgcgaccag ccctaccaga tggaagccac caccgagcaa cacaccgccg
    16801 gccagccgcc acacaacacc cgcagcgtcg tactcaagtt ctttcaggac ctgggcgggg
    16861 cgcacccgtc caattggttc aaggcattca actacaacct cgggaccaag cagcctatca
    16921 ccttcgacaa tcttttcccg cccggcacga cgccgctgga caccatcttt ccgatcgtgc
    16981 agcgcgacct ggagcggcaa aacgcgttgg gggtggcgat attgccgtcg acggggcttg
    17041 acccgtcgca ctatcaaaac ttcgccatca ccgatgacca gctgatcttt tacttcgccc
    17101 ccggcgagat gctgccggcg ctcgccggac cggtccaagc ccaggtgccg cgcaacgcga
    17161 tcccgccgct tgcggtctga cgaacctaaa cgggcccggc gagcgactcc agcgccgcgg
    17221 cgaggatcgc cagcacgacc tgcgcgacgc aaccgatgat cgggacgaag aacgccattc
    17281 tgcggcgcag gaggagatag ccggcgacca ggaagtcggc gatcaggact gcggcgccgg
    17341 cccaaatccc caatgtcatg gcgcggtcga cccaggcggg atcgccgcac ttcacggcac
    17401 cgcacgcgtc ggttcccatg acaaacaggc ccagcagcac gacggtcgcg ccgaacagga
    17461 gtccgtggac tatgagcaga accagggtcg cggcgacgtc cgcgggccgg ctgccgtggc
    17521 cgcgacgagc atgcgcgctc gccgggttct gcggcgcggg gtcgtggttg gggtcaggcc
    17581 ggggcaaagc tatagacctg accgtcgctg gtggccgcca ccactcggcg gtcgttgcca
    17641 accgacaccc cgatggggta tccggtggcc gccggaagcg gatagctgtt gagcgtatgg
    17701 ccgttggccg gatcgaagac caggaccgac atgccgggtt cgccgtccgc gggcgggccg
    17761 ctgaccacgg tgtagccgac gccgacgccg gccacgctcg gggacgacag cggagtgacg
    17821 tcatcgcggc gccagaccgg atcggcgtga tcgccggcgt ccttgaatgc cgcgagccgg
    17881 gtttcgggcc cgccgcccga gacgatcagc ccctgcgggg tgaccgccgg cggcgtctgc
    17941 gccaggaagc ccagcggcac cgaccacttc actttcccgt cggcggcgga cagggcccac
    18001 agccgttggt cgcggccgtc gacgtagacc gtggacccgt cggcggacaa caccgggctg
    18061 gcgatgacgc cggcgcctac cgcgtcgctg gtccactccc gcaccaggag cggggtctgc
    18121 cccgggtgat atttgagccc gaccagtccc gccgccggag cgcccggctg ccagacgccc
    18181 agcaccaccg tcccgttggc ggtcgagaag gccggcgccg ccgcgagcgg gcagccctgc
    18241 cgggcgggcg cgcagtcggc caacccgcgc gtcgcgtcgg tggggtcgat gccctccacc
    18301 aggtcgagcg ggctgccgac cacctcgccg cggtgggcgt cgaaaaccag cacttgaccg
    18361 aggtgggtgg tgacgagcag ttggccgtgt cccaggaacc gcggggtggc cggcatcccg
    18421 atcaccggct tgcgccaccg cgtccactgg gtcaccggaa aggagataaa tgcgcccggt
    18481 tgaccgacgt agaggttgtc gaagccgtcg aacaacggtc cggcgaagcc gccgccctgg
    18541 aaaagccgaa cgcaccagcg ttgccggcct ttgtcgttgt tttcccactg catcagcgaa
    18601 cacccggccg gggtctgcgc gttgagggcg agatatccgc gtgcgctcag cgccggaccg
    18661 gccgccaagc tacctttgac cgaccgcgtc cactgcaacg agagcttgct cgcaccgctc
    18721 gtcgcggtgt agctgctgtt ggccgcgtcg ccgtattgtg ccggccagcc ttgcgcgggc
    18781 gacgcgtcga cccacgagtc ggtgttgccg caaccgccga gtccgaccgt gagcgcggcc
    18841 gccagcatcg ccgccgacca acgccgaagc ccactgacga gttgtggggc aagcacctcg
    18901 gtttcccaaa tccttccgcg acgcgggcag cgatcgagta caggtgaggg taactcgtgg
    18961 cgactcgggt gtatcggctt gcccgccttc tcagcccgcc gtttcggcgg gcatcgtcgg
    19021 cggcggtgtc tggttgcccg cctccttaac ccgccgtttc ggcgggcatc gtcggcgggc
    19081 taggctttcc ggccatgacg agcatgtggg gtgccccgtt ccatcgccga tggcgtggat
    19141 cgaacctgcg ggaccctcgc caagccaggt ttctcacgct ggcgtcgctg aaatgggtgc
    19201 tggccaaccg cgcgtacacc ccgtggtacc tggtgcgcta ctggcggttg ctgaagttca
    19261 agctggccaa cccgcacatc atcacccgcg ggatggtgtt cctcgggaag ggtgtggaga
    19321 tccatgccac acccgaactg gccgagttcg agatcggccg ctgggtgcac atcggggaca
    19381 agaacaccat ccgcgcacac gagggctccc tgcggttcgg cgacaaggtg gtgctgggcc
    19441 gggacaacgt gatcaactgc tacctcgaca tcgagttcgg ggattcggcc ctgatggccg
    19501 actggtgcta cgtctgcgat ttcgaccacc gcatggacga catcaacgtc ccgatcaagg
    19561 atcagggcat catcaagtcg ccggtccgaa tcgggccgga cacctggatc ggggtcaagg
    19621 cgaccgtgct gcgcggcacg tccatcgggc gcggctgcgt ggtggggtcg cacgccgtgg
    19681 tccgcggcgt catccccgac tactcgatcg ccgtcggcgc gccggccaag gtggtcaaga
    19741 accggcggct gtcctgggag acctcggccg cgcagcgtgc tgagctggcc gccgctctgt
    19801 ccgatatcga gcgcaaaaag gcggccaggt aggcgatcat tccgggtggt cgtccacctt
    19861 gaaatcgaag ttgacgttcc cggcgtcgac gtttctgacg atcacggcta cgccgtactt
    19921 ctgatccgag tcggtgagtt cacaccgcag cgtcgcgccc tggacaccct tcaagttgtc
    19981 cgggcaggtc accgaatcgg gcttgcggcc caactgctga gtcagctcgt agctgatcac
    20041 gttggcgacc tggttcttgt cgacggtctc cgccaggtcg aacttgacgt cgtcgccgtt
    20101 gacgttggtc acggtcacgt tgacggtgta ggtctggtcc ttgaccttca tcgtgcaatt
    20161 cagctgtgcc ccgaccttcg ccggcaggtc gctggggcac gtcaccgaat cgggtttgtt
    20221 gccggcggca tcggtcaact tggaagtgat ctggttggcg atatcggttt tgctgacggc
    20281 gtgcgaggac gatccgatcg aacaggaaca ggctccggtg ccggccgcca acccggcggc
    20341 agcgcccgca accagcaacg tccgaacgat cgaatgagcc attagtgcct cccgagccaa
    20401 ccggtaactg tgaatcggct gataattgca tgccaaattg cttcgggtag gtgaattcgc
    20461 taaaccttca tcgaacgacg atgggtcgct cagatgctct gataccgccg cagggcttct
    20521 gcccgctcga caccgtggtc gacgatcggc gctgggtacg cctgcggccg ttcgcctttg
    20581 cgcaggtgcg ggtcgtcggc cgagcgcagc tcgggaaccc agcgccggat gtaggagccc
    20641 gacggatcga acttctcgcc ctgcgcagcg gggttgaaca cccggaagta cggcgcggcg
    20701 tcggtaccgc atcccgcgca ccactgccat ccgtgctggt tgctggccag gtcgccgtcc
    20761 acgagttgat ccaggaacca ttcggcgccc cactgccacg gcaaatggag gtccttgacc
    20821 agaaacgacg ccacggtcat ccgcacccgg ttgtgcatga agccggtctc gcggagctga
    20881 cgcatcccgg catcgacgaa gggaaacccg gtcttgccgg acttccaggc ctcgaagcgg
    20941 cgttcggctt cggctccggt gtcgttcgcg atggcgtcga aatcgctgtt ccaattgcgc
    21001 caggcgctgg ccggccagtg atgcagtaca tcggcgtaga agtcccgaaa tgccaactcg
    21061 cgcagatacg ctcgtgcccc cgcgccacgc aagtcgaggt cggcgaccat ggtgcgggga
    21121 tggatggtgc cgaacttcag gtgcgccgac atccggctgg tgccgttgcg atcgggcctg
    21181 tcgcggtcac gatcgtagcg ttctaatccg ttgtccacga acgacttcca aagccgggtt
    21241 gccgccgcct caccggcgcc gacgtcgagt gcagcgcctg aatccgggat cgtgcaggtc
    21301 tgattcccag ctgcgcagga tccagccagc gcgcggactg tgcgcccgat ttcgccgacg
    21361 cccgccagcc gacctcgcgc caccggcgga gaaacggtgt gaacacccgg tagggggagc
    21421 cgtccttctt ggtgacacgc cccggtgaaa ccaggtacgg cgaacccgtc gcccgcaacg
    21481 gcacagaatc cagcgcctcg cgcactcgtt cgtcacgccg ctttccgaac ggtgcgaaat
    21541 cctccgaaat gtgcaccgcc gatgcgtcga tttccttggc aatgcgcgga atctgccgct
    21601 cgggctgtcc ccggaccacg agcagccgac cgtccaggtc gtctcgcagt tgccgcagcg
    21661 agtcgcccag gaattgcagc cgtcgccggc ccgacgaagc ctccagtcgc ggatcgagca
    21721 caaagcaggc gaggacttcg ccgtcgtcgg ccgcggccgt cagggcgggg tgatcgccca
    21781 gccgcaggtc gcggcgaaac cacaacagcg tcggcatgat tcgatgcttc ctcagcggtt
    21841 gagccgccag acatgggtgg caaggacggt ggcgaaggcg ccccacaacg ggtaggcgga
    21901 cagcgccgat cttgcgcgcc ggtcggcctc cgcggcccgg cgggcaagat cggcgctgct
    21961 ggccgtcagc gccgcggcgc caagagcgga ggcgccgagc ttgtgatagt ggaagaagag
    22021 ccaagtccat ccggcgttca gcagcaggtt caggctgagc gccgcggcgt agcggcgtgc
    22081 cttgtcgtgc tgacccgcgg cgcgcaaccg gtcgatggcc accgcggagg tggccgcgat
    22141 gttgccgtaa agcgtggtcc agaccacagg gaacgcggcg ccgggcggct ggtaggacgg
    22201 cttgcgcagc cgcgagtacc aggcgggtac ccgcttcggg ctggcgatgc taccggtgcc
    22261 ggccgccgtt gcgacggcga gcgttgtggc ggccagagtc gacttcttca cactgtgctc
    22321 cgttctcgtg aggccgaatc cgcttggttt tcactatctt tggctggaag tcatcccgac
    22381 ggcaggcttg cgccgccgct gccggcgtcg ccgtcgcacc cattaccgag catttcagtt
    22441 atttaaccat taagcaagtt aactaatgcc ggagcgccga aatcggtggc ccagcggtga
    22501 cgccgctgtg ggcctgatcg agcagcgccc gcg
//
LOCUS       DASSGQ010000354        13369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_178501,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000354 DASSGQ010000000
VERSION     DASSGQ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13369
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178501"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VFI55_15760"
     CDS             <1..823
                     /locus_tag="VFI55_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7075519.1"
                     /translation="CVASMLIAQAAMRPVRRLEEGTRKISSGDYDINIPVMAHDEIGD
                     LTAAFNEMSRNLAIKEELLNEQRSENNRLLLALMPESIVRRYRDGEATIAEKHQDVAI
                     IYADIVGLDEILNDLPGDELVTIVDDLFRQFDSAAETLGVERIRTFHNGYLASCGVIT
                     PRLDSTHRSVEFALEMRRIIERFNNQTGHDLGLRVGINTGNVISGLVGRSSLVYDMWG
                     AAVSLAYQMHRGAPQPGIYVSSQVYEAMRDVRQFTPAGTISVGGTDQTIYRLSER"
     gene            823..2250
                     /locus_tag="VFI55_15765"
     CDS             823..2250
                     /locus_tag="VFI55_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET7075520.1"
                     /translation="MNAWHSSWFYWAIGIAIGLPLGLIILTELHHALVRRGSRLAAQV
                     GLLRNYVLPLGALLLLLVKASQIPAGEVPVRMLTTIFSFLLLVLLLSGFNATVFDGAP
                     EESWRKRLPAIFLDVGRFAVIGLGLALILSYVWGVRVGGLFTALGVTSVVIGLMLQNS
                     VGQIVSGLFMLFEQPFRINDWLDTGTARGRVVEVNWRAVHIETGGGMRIMPNSALATT
                     SFTNLSRPPGAYKLAMTTTFSAADPPDQVCAMLSRVACALPQLKVDSVPRSVPVGGAE
                     YRTTIGLNSPTDDGTARATFLRWIWYAARREGLHLDDSADDYSTPERVEKAVRTVAAP
                     ALRLNADEQRSLHSCARLVRYGADEIVEYAGQVPAGMTFLVAGRVQLTATADDGSVVP
                     ISVLVEGSFLGLTALTRQPNMGSAYALEEVTALEIDREHLEHLVMREPLLLQNFGHIL
                     DERQSKVRRARSGERVGDAPVRPRS"
     gene            2317..3564
                     /locus_tag="VFI55_15770"
     CDS             2317..3564
                     /locus_tag="VFI55_15770"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET7075521.1"
                     /translation="MSLQAPSRPDLRRDVHNAARRARVAARALALLPTAAKDAALKSA
                     AAAIVDHTGQILTANAQDLDAARVAGTPAAMLDRLALDPKRVDGIAAGLRQVAGLPDP
                     VGEVLRGYTLPNGLQLRQQRVPLGVVGMIYEGRPNVTVDAFGLALKSGNAVLLRGSSS
                     AAKSNQALVDVLRASLAGEDLPADAVQLLSAADRSTVTHLIQARGLVDVVIPRGGASL
                     IDAVVRDAQVPTIETGVGNCHVYVHEGADLAMAERILLNSKTRRPSVCNAAETLLVDA
                     KIAREAVPRLVAALRDAGVTVHLDAAEDDLRREYLSMDIAVAVVDGVDAAIAHINEYG
                     TGHTEAIVTTNMAAAQRFTDGVDAAAVMVNASTAFTDGEQFGFGAEIGISTQKLHARG
                     PMGLPELTSTKWIAWGDGQIRPA"
     gene            3599..4474
                     /locus_tag="VFI55_15775"
     CDS             3599..4474
                     /locus_tag="VFI55_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET7075522.1"
                     /translation="MSVPARPAHLFADIDDVSRKLAETGYLPDTATATAVFLADRLGK
                     PLLVEGPAGVGKTELARAVAQATGSGLVRLQCYEGVDEARALYEWNHAKQILRIQAGS
                     GDWEATKTDVFSEEFLLSRPLLTAIRRTEPTVLLIDETDKADIEIEGLLLEVLSDFAV
                     TVPELGTLTAARAPFVLLTSNATRELSEALKRRCLFLHIDFPSPDLERRILLSRVPEL
                     PAHLAEELVRIIGVLRGMQLKKVPSIAETIDWGRTLLALGLDTIDDAVVAQTLGVVLK
                     HQSDQQRASGELRLN"
     gene            4474..5922
                     /locus_tag="VFI55_15780"
     CDS             4474..5922
                     /locus_tag="VFI55_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7075523.1"
                     /translation="MAARRTRPTRPLAPHGLPGHLVGFVEALRAHGISVGPSETVDAG
                     RVMATIGLGDREVLREGIACAVLRRPEHRETYDAMFDLWFPAALGTRAVVLDDAADGA
                     DGGGALPPDDVEAMRQMLLDLLTQNPDLADMDERLVAMIARIVEAYGKYSSSRGPSFS
                     SYQALKAMALDELEGKLLAGLLAPYGDEPTPTQEQIAKALAAQRISQLRKMVDAETKR
                     RTAEQLGRDHVQMYGIPQLSENVEFLRASGEQLRQMQRAVAPLARTLATRLAARRRRA
                     RAGSIDLRKTLRKSMSTGGVPIDVVLAKPRPARPELVVLCDVSGSVAGFSHFTLLLVH
                     ALRQQFSRVRVFAFIDTTDEVTHMFGPEADLAVAIQRITREAGVYGRDGHSDYGNAFV
                     SFVQANPNALSPRSSLLVLGDGRTNYRDPAIDVLADMVTASRHAHWLNPEPKHLWGSG
                     DSAVPRYQEVITMHECRSAKQLAAVVDGLLPV"
     gene            6342..7022
                     /gene="nadD"
                     /locus_tag="VFI55_15785"
     CDS             6342..7022
                     /gene="nadD"
                     /locus_tag="VFI55_15785"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET7075524.1"
                     /translation="MQNQLRRLGVMGGTFDPIHYGHLVAASEVADLFDLDEVVFVPSG
                     QPWQKSRHVSAAEDRYLMTVIATASNPRFSVSRVDIDRIGPTYTRDTLRDLHALNPDS
                     ELYFITGADALASILSWQGWEELFTLARFIGVSRPGYELRHDHITGVLGDLTEGALTL
                     VEIPALAISSTDCRRRAEQRRPLWYLMPDGVVQYVSKRRLYRQSRDNKAKRSTNSPAS
                     RLATGNNT"
     gene            7019..7423
                     /gene="rsfS"
                     /locus_tag="VFI55_15790"
     CDS             7019..7423
                     /gene="rsfS"
                     /locus_tag="VFI55_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET7075525.1"
                     /translation="MTATPEAIDMATVAAAAAAAKLASDVVVIDVSGQLVITDCFVIA
                     SASNERQVNAIVDEVEEKMRKAGYRPARREGAREGRWTLLDYRDIVVHIQHQDDRNFY
                     ALDRLWGDCPVVPVDSAEDPGRGDQADSASAQ"
     gene            7420..8094
                     /gene="gpgP"
                     /locus_tag="VFI55_15795"
     CDS             7420..8094
                     /gene="gpgP"
                     /locus_tag="VFI55_15795"
                     /EC_number="3.1.3.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate phosphatase"
                     /protein_id="HET7075526.1"
                     /translation="MRIRRLVMLRHGQTDFNAGSRMQGQLDTELTELGRAQAVAAAEV
                     LGKMQPLLIVSSDLRRAYDTAIKLGERTGLKVSVDDRLRETNLGDWQGMTHAQIDAEA
                     PGARLAWREDATWAPHGGESRVDVAARSLPLVGELVSGEPEWGDSNQPERPVVLVTHG
                     GLIAALSAALLRLPVANWPILGGMGNASWVQLSGHSEDSDVDFEPIRWRLDVWNASAQ
                     VSNDVL"
     gene            8084..8833
                     /gene="octT"
                     /locus_tag="VFI55_15800"
     CDS             8084..8833
                     /gene="octT"
                     /locus_tag="VFI55_15800"
                     /EC_number="2.3.1.273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016414 - O-octanoyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosylglycerate octanoyltransferase"
                     /protein_id="HET7075527.1"
                     /translation="MSSDPAVSSRPALLVFADSLAYYGPTGGLPADDSRIWPNIVASQ
                     LDWDLELVGRIGWTCRDIWWAATQDPRAWAALPRAGAVIFATGGMDSLPSVLPTALRE
                     LIRYVRPPWLRRWARDGYGWLQPRLSPVARSALPPHLSAEYLELTRGAIDFNRPGIPI
                     VASLPSVHIAETYGKAHQGRAGTVAAITEWAQGHNVPLVDLKAAVAEQIMSGRGNPDG
                     IHWNFEAHQAVAELMLKALAEAGVPNEKPRG"
     gene            8963..9796
                     /locus_tag="VFI55_15805"
     CDS             8963..9796
                     /locus_tag="VFI55_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET7075528.1"
                     /translation="MTVVVVTDASSRLPGDLLEKWSIRVVPLHILLDGHDLRDGVDDI
                     PEDIYKQHATTAAATPAELADTYQQALADSGGDGVVAVHISSALSGTCGAAQRTAADL
                     DPSVRVIDSRSAAMSTGFVALAAARAAAAGADLDAVTEAASSAVSRSHAFVVVHRLDN
                     LRRSGRIGGATAWLGTALALKPLLRIDDGKLVLAQRVRTVSHATAAMIDRVCEVVGEG
                     SAALAVHHVANPDGADEVAAALAERLPNCEPAIVTALGPVLALHVGAGAVAVCLELAA
                     T"
     gene            complement(9813..12719)
                     /locus_tag="VFI55_15810"
     CDS             complement(9813..12719)
                     /locus_tag="VFI55_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET7075529.1"
                     /translation="MSDLNNKSHVTTEDQDTGPIGTPSRAKPERGHRPVIPHTIRIFA
                     VPIILGWVFVTVLVNVIVPRLEIVGEAHSAPMTPLDAPSMKAMMLLGHNFHEFDSNST
                     VMIVLEGQQPLGDEAHRYYDSLVRQLRADPQHIQHIQDFWGDRLTAAGAQSADAKGAY
                     VMLNLAGNQGTTQANDSVDAVRKVIDRNQPPPGVKAYVTGPAALSDDMHLIGNASLAK
                     ITLFTLGAIAIMLLLVYRSIVTTLVQLFMTFVALGCSRGVVAVLAYHNAFGLTTFAAN
                     ILTMLAIAAGTDYGIFLIGRYQEALRAGEDRETAYYTTFRGVAPVVLGSGLTIAGATY
                     CLSFARLPWFNTMGAPVAIGMLVVVLAGLTLGPAVVFVGSRFHLFESKRAAKGGRLWR
                     RVGTAVVRWPAAVLAVSSAIVLVGMVALPSYKTSYNDRYYLPTSAPSNLGQAAADRHF
                     SQARMNPDMLMVEADHDMRNPADMLVLDRVAKNVMRTVGIAMVQDITRPLGIPIQHSS
                     IPFQNSMQSQTTMQNMAFLKDRMADILKLADQLQVNIDVTERQYAVTQDLSKAADDSA
                     RTTAETRDITDSLRNHIADFDDFWRPIRAYFYWDKHCFDIPICWSLRSIFESFDGIDQ
                     LAEKFHDLTADIQHTASATHELMTLFPTLIATLKTTRGITLTLYQTFSAMINQIEAMS
                     NTAVVMGQSFDASKNDDFFYLPPEAFDNPDFQTGLRMFLSPDGKSARFFITHQGDPMT
                     PEGISRVDSERTAAQEGLKQSSLSDAKVYLGGTAATFKDMADGEKYDLMIAVVSALTL
                     IFMIMLLLTRSVVAALVIVGTAASSIAASFGLSVLIWQDLFGIKIHWIVAALSVIILL
                     AVGSDYNLLLVSRFKEELHGGIKTGIIRSMAGTGGVVTAAGLVFAFTMASMLGSDLRV
                     LGQFGSTVCIGLLLDTLVVRTLLMPSIATLLGRWFWWPQVVHPRGDNARRPVRA"
     gene            complement(12716..13144)
                     /locus_tag="VFI55_15815"
     CDS             complement(12716..13144)
                     /locus_tag="VFI55_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmpS family transport accessory protein"
                     /protein_id="HET7075530.1"
                     /translation="MVRVVKRAWIPLLLIVVLAVSALVVTRLHKIFGSQDLNANAGAG
                     IEIVQFNPKVVVYEVSGPPGATANINYWDENANTHQVDAAPLPWSTTISTTLPAVSAN
                     IMAQSDGSRISCTITVDGVVRDRQNSDGHNAQTFCLVKSA"
BASE COUNT         2251 a         4301 c         4518 g         2299 t
ORIGIN      
        1 ctgcgtggcc tcgatgctga tcgctcaggc cgcgatgcgg ccggtgcggc gcctggagga
       61 gggcacgcgg aagatcagct ccggcgacta cgacatcaac attcccgtga tggcacacga
      121 cgaaatcggt gatctcaccg cggcgttcaa cgagatgagc cggaatcttg cgatcaaaga
      181 ggagctgctc aacgagcagc gcagcgaaaa caatcgcctg ttgcttgcgc tgatgcccga
      241 gtcgattgtc cgacggtacc gcgacggtga ggcgaccatc gccgagaaac accaggacgt
      301 cgccatcatt tatgccgaca tcgtcgggct cgacgagatc ttaaacgatc ttcccggcga
      361 cgaactggtc acgattgtcg acgatctgtt ccggcagttt gattcggcag ccgaaaccct
      421 tggcgtggag cggattcgca cattccacaa tggctacctc gccagctgtg gcgtcatcac
      481 tccgcgattg gacagcactc accggagcgt tgagttcgcc ctcgaaatgc ggcgcatcat
      541 cgagagattc aataaccaga ccggtcacga cctcggcctt cgggtcggca tcaacaccgg
      601 caacgtcatc agcgggctgg tcggtcggtc cagcctggtc tacgacatgt ggggcgccgc
      661 ggtgagtttg gcctaccaaa tgcaccgcgg cgcaccgcag cccggcatct atgtcagctc
      721 tcaagtctat gaagcgatgc gcgacgtgcg tcagttcacg cccgccggca cgatttcggt
      781 cggtggaacc gaccagacga tctaccgact gtcggagcga tgatgaacgc gtggcactcc
      841 tcatggtttt actgggcgat tggcatcgcg atcggattgc cgctcgggct gatcattctc
      901 accgagctgc accatgcgct cgtccgcagg ggtagccgcc tggccgcgca ggtaggcctg
      961 ctgcgcaact acgtattgcc gctcggcgcg ctactgctgc tgctcgtcaa ggcctcgcag
     1021 atcccggccg gagaagtgcc ggtgcggatg ctgacaacga tcttcagctt cctgctgctg
     1081 gtgctgttgc tctccgggtt caacgccacg gtgttcgacg gcgcgcccga ggagagctgg
     1141 cgtaaacgat tgcccgccat cttcctggac gtcggtcgct tcgcggtgat cggcctcggg
     1201 ctggcgctga tcctgtcgta tgtctggggt gtccgggtcg gcggcttgtt caccgcgttg
     1261 ggcgtcacgt cggtcgtcat cggcctgatg ctgcagaact ccgtcggcca gatcgtgtct
     1321 ggcttgttca tgctgttcga gcagccgttc cggatcaacg actggctgga cacggggacg
     1381 gcgcggggac gcgttgtcga agtgaactgg cgggcagtgc acatcgaaac cggcggcgga
     1441 atgcgcatca tgccgaactc ggcgctggcc acgacgtcgt tcaccaatct cagtcggccg
     1501 ccgggcgcct acaaattggc gatgacgacg accttttcgg ccgccgaccc gcccgatcaa
     1561 gtttgcgcga tgctgtcgcg tgttgcttgc gcgctgccgc agctcaaggt cgacagcgtg
     1621 ccccgctcgg tgcccgtcgg gggtgccgaa taccgcacga cgatcgggct gaactcgccc
     1681 accgacgacg gcacggcgcg cgccacattt ctgcgctgga tctggtacgc agcgcggcga
     1741 gaggggctac atctcgacga cagcgccgac gactactcga cgcccgaacg ggtggagaag
     1801 gctgtgcgca ccgtggcggc gccggcgttg cggctgaacg ctgacgaaca gcggtcgctg
     1861 cattcgtgcg ccagactggt ccgctacggt gccgacgaaa tcgtcgagta cgccggtcag
     1921 gtgcccgcgg gaatgacgtt cctggtcgcc gggcgcgtgc agttgaccgc gacggccgac
     1981 gacgggtcgg tggtgccgat cagcgtcctg gtcgagggct cattcctcgg cctgaccgcg
     2041 ttgactcggc aaccgaacat ggggagcgca tacgcgctcg aggaagtcac cgcgttggag
     2101 atcgatcgcg aacacctcga gcatcttgtc atgcgtgaac cgttgctact acagaacttt
     2161 ggccacatcc tcgacgagcg gcaaagcaaa gtgcggcggg cccgcagcgg agagcgggta
     2221 ggcgacgcac cggtgcgtcc gcgctcctga ggtgtgatgc cagcacccgg gtgggccagg
     2281 ctgaccgggc cggccatgga ccgataccct ggataaatga gtctgcaagc tccatcgcgc
     2341 cccgacctgc gccgggacgt ccacaacgcc gcacgccgcg cgcgcgtcgc ggcccgggcg
     2401 ctggcgctgt tgccgacggc cgccaaggat gcggcgctga agagcgcggc agccgcgatc
     2461 gtcgaccaca ccgggcagat cctgacggcc aacgcccagg atctcgatgc tgcccgggtc
     2521 gcgggcacac cggccgcgat gctcgaccga ttggcgctgg accctaagcg cgtcgacggc
     2581 atcgccgcgg gtctgcgaca ggtggccggg ttgcccgacc cggtcgggga ggtgctgcgg
     2641 gggtacaccc tgccgaacgg gctgcaactg cgccagcagc gggttcccct gggcgtggtc
     2701 ggcatgatct acgagggccg gcccaacgtc accgtcgacg ccttcggatt agcgctcaag
     2761 tccggcaatg cggtgctgct gcgcggaagt tcgtcggcgg ccaagtccaa ccaggcgctc
     2821 gtcgacgtgt tgcgtgcttc gctggccggc gaggacctgc ccgccgacgc ggtccagttg
     2881 ctttcggccg ccgatcgatc caccgtcacc cacctgatcc aggcccgcgg tttggtcgac
     2941 gtggtgatcc cgcgcggggg agcgagcctg atcgacgcgg tggtccgcga cgcgcaggtg
     3001 cccaccatcg aaaccggcgt cggcaactgc cacgtctatg tacacgaagg cgccgacctg
     3061 gccatggcgg aacggatcct gctgaactcc aagacccggc ggcccagcgt ctgcaacgcc
     3121 gccgagacgc tgctcgtcga tgccaagatc gcccgcgagg ccgtgccccg gctggtggcc
     3181 gccctgcggg acgccggcgt gacggtgcac ctggacgccg ccgaagacga cctgcgccgc
     3241 gaatacctgt cgatggacat cgcggtggcg gtggtcgacg gcgtcgatgc cgcgatcgct
     3301 cacataaacg agtacggcac cggacacacc gaggccatcg ttaccaccaa catggctgcg
     3361 gcccaacggt ttaccgacgg ggtcgacgcg gcagcggtga tggtgaacgc ctcgacggcg
     3421 ttcaccgacg gtgagcagtt cggtttcggt gccgagatcg gcatctccac gcagaagctg
     3481 cacgcacgcg gaccgatggg gttgccggaa ctgacctcga ccaagtggat tgcgtgggga
     3541 gacgggcaga tccgcccggc ctgacactcc cgaccaaaag ctggaggaga acacgattgt
     3601 gagcgtgccc gcccgccccg cccatctgtt cgccgacatc gacgacgtct cacgcaagtt
     3661 ggccgagacc ggctatctgc cggacaccgc caccgcgacg gcggtgtttc tggccgaccg
     3721 gctcggcaag ccgctgctcg tggagggccc cgccggcgtc ggcaagacgg agctggcccg
     3781 cgccgtcgcc caggccaccg gctccggcct ggtgcgcctg cagtgctacg agggcgtgga
     3841 cgaggcgcgt gcgctgtacg agtggaacca tgccaagcag atcctgcgca tccaggccgg
     3901 ctcgggcgat tgggaagcca ccaaaaccga tgtcttcagc gaggagttcc tgttatcccg
     3961 tccgctgctg acggccatcc ggcgcaccga gccgacggtg ctgctgatcg acgagacgga
     4021 taaggccgac atcgagatcg aaggcctgct gctggaggtg ctgtccgatt tcgcggtgac
     4081 cgtgcccgaa ttgggcacgc tcaccgcggc gcgggcaccg ttcgtgttgc tgacctccaa
     4141 cgcgacccgc gaactgtcgg aggcgctcaa gcgtcgctgc ctgttcttgc acatcgactt
     4201 cccgagcccc gacctggagc ggcgcatcct tctatcccgc gtccccgagc tgcccgcgca
     4261 cctcgctgag gagctggtgc gcatcatcgg tgtgctgcgc gggatgcaac tcaagaaggt
     4321 gccgtccatc gccgaaacca tcgactgggg ccgcacgctg ctcgccttgg gcctggacac
     4381 gatcgacgac gcggtcgttg cccagacgct cggggtggtc ctcaagcatc aatccgatca
     4441 gcagcgcgcg tccggggagt tgaggctgaa ctgatggccg cccgccgcac ccgcccgacc
     4501 aggccgctcg ccccgcacgg gctgcccggc cacctggtgg gttttgtgga ggcgcttcgt
     4561 gcacatggga tttcggtggg gccatcggag acggtggacg ccgggcgggt gatggccacc
     4621 attggcctcg gcgatcgcga ggtgctgcgt gagggcatcg cctgcgcggt gctgcgccgt
     4681 cccgaacacc gcgagaccta cgacgccatg ttcgacctgt ggtttcccgc ggcattgggc
     4741 actcgggcgg tggttctgga cgacgctgcg gacggggcgg acggtggcgg cgcgttgccg
     4801 cccgacgacg tcgaggcgat gcgtcagatg ctgctggatc tgctcaccca aaaccccgac
     4861 ctcgccgaca tggacgagcg cctggtcgcg atgatcgcgc ggatcgtgga ggcctacggc
     4921 aaatacagtt ccagtcgcgg cccgtcgttt tcgtcgtatc aggcgctcaa ggcgatggcg
     4981 ctggacgaac tggagggcaa gctgctggcg ggactgctcg ccccgtacgg tgacgagccc
     5041 acacccaccc aggagcagat cgccaaagcg ctagcggcgc agcgtatttc acagttgcgc
     5101 aagatggtcg acgcggagac caagcgccgc acggctgagc agctgggccg cgaccacgtt
     5161 cagatgtacg gtatcccaca gctttcggag aacgtggaat tcctgcgcgc ctcaggtgag
     5221 cagctgcgcc agatgcaacg tgccgtggcg ccgctggccc gcactctcgc aacccggctg
     5281 gcggcccgcc ggcgccgcgc ccgcgccgga tcgatcgatc tgcgcaagac gctgcgcaag
     5341 tcgatgtcca ccggtggcgt gccgatcgac gtggtgctgg ccaagccccg ccccgctcgc
     5401 ccggaactgg tcgtgctgtg cgacgtgtcc ggatcggtcg ccgggttcag ccacttcacg
     5461 ctgctgctgg tccacgctct gcgccaacag ttctcgcgag ttcgcgtctt tgccttcatt
     5521 gacaccaccg acgaggtgac gcacatgttc gggccggaag ccgacctggc ggtggcgatc
     5581 cagcggatca ccagggaggc cggtgtctac ggccgcgacg gccattccga ctacggcaat
     5641 gcgttcgttt cgttcgtgca ggccaacccg aacgcgctgt cgccgcgcag ctcgctgctg
     5701 gtgctcggcg acgggcgcac caactaccgc gacccggcca tcgacgtgct ggccgacatg
     5761 gtgaccgcca gccggcacgc gcactggctc aaccccgaac ccaaacatct gtggggcagc
     5821 ggcgactcgg cggtgccgcg ctaccaagag gtgatcacga tgcacgagtg tcggtccgcc
     5881 aagcaattgg cggcggtggt cgacgggctg ctgccggtgt gagaccgcgc agcgtcgctg
     5941 tcgcggcagt tggcaccgca tctcgtcaca tcggccacac gcgtctccgt ctggacggac
     6001 ccgcgttgtg cccgcctcac agtgactgga gcagggcttc cctttcgagt gtgcaggggc
     6061 gtcgaccgtc ggcccggcgc catcgagtct gcgatgagcg cgcgatcccc ggcttcaggt
     6121 gggcgctaca cgcagagtcg aatccctcag tacagactcg atgcataatg cgagccgtca
     6181 agctctcgcc cattcgaaat gggtcgccga gacatttggg tgtcgtagcg aggcggcaaa
     6241 attcatatcg cccgctgcac aagcgcatgg ggcgcggcgt cggtcgggtg gccgagccgg
     6301 gaaatcgggt gtgatcaccg cctgaagtaa gctggcacat cgtgcaaaat cagcttcgca
     6361 ggttgggagt aatgggtggg accttcgatc ccatccatta cggccacctg gttgccgcca
     6421 gtgaggtcgc ggacctgttc gacctcgatg aagtggtgtt tgtgcccagc gggcagccat
     6481 ggcaaaagag ccggcacgtg tccgctgccg aggatcggta tctgatgacg gtgatcgcca
     6541 ccgcgtcgaa tccacgtttc tcggtgagcc gggtggacat cgaccgcatc gggcccacct
     6601 acaccaggga cacgctgcgc gatctgcacg ccctcaaccc cgactccgag ctgtacttca
     6661 tcaccggcgc cgacgcgctg gcctccattc tgtcgtggca agggtgggaa gagctcttca
     6721 cgctggcgcg cttcatcggc gtcagccggc ccggctacga gctgcggcac gatcacatca
     6781 ccggcgttct gggcgacttg accgagggcg ctttgaccct ggtcgagatc ccggcgctgg
     6841 ccatctcgtc gaccgactgc cgtcggcgcg ccgagcagcg ccggccgctg tggtacctga
     6901 tgcccgacgg agtcgtgcaa tacgtctcca agcgccggct ctaccgccag tctcgtgaca
     6961 acaaggccaa gaggtccaca aattcgccgg cctcgcgcct ggccaccggg aacaacacgt
     7021 gaccgccacc ccggaagcga tcgatatggc gaccgtggcc gccgccgcgg ccgccgcaaa
     7081 gctggccagc gacgtggtcg tcatcgatgt gtcgggccag ctggtcatca cggactgctt
     7141 cgttatcgcc tcggcgtcca atgagcggca ggtcaacgcc atcgtcgacg aggtcgagga
     7201 gaaaatgcgc aaggcgggct accggcccgc gcgtcgcgaa ggtgcccggg aagggcgctg
     7261 gaccctgctg gactaccgcg acatcgtcgt gcacatccag catcaggacg accgcaactt
     7321 ctatgccctg gaccggctgt ggggtgattg cccggtggtg ccggtggatt cggccgaaga
     7381 tcccgggcgg ggcgatcaag cggactcggc gagcgcgcaa tgagaatccg ccgtctggtg
     7441 atgctgcggc acgggcagac cgatttcaac gccggtagcc ggatgcaggg ccagctggac
     7501 accgagctca ccgagctggg ccgcgcgcag gcggtcgcgg ccgccgaggt gctgggcaag
     7561 atgcagccgc tgctgatcgt ctcgtcggac ctgcggcgtg cctacgacac ggcgatcaag
     7621 ctgggggagc ggacggggct gaaggtctcg gtggacgacc ggttgcggga gaccaacctc
     7681 ggcgactggc agggcatgac gcacgctcag atcgacgccg aagcaccggg ggcccggctg
     7741 gcatggcgcg aagatgcgac gtgggcgccg cacggcgggg aaagcagggt cgacgtcgcg
     7801 gcccgcagtc tgccgctggt cggcgagctg gtatccgggg aaccggaatg gggcgactcg
     7861 aatcaaccgg aacgaccggt ggtgttggtg actcacggcg ggctgatcgc cgcgttgtca
     7921 gctgcgttgc tgaggcttcc cgtcgccaac tggccgatcc tgggcgggat gggcaatgcc
     7981 agttgggtgc agctcagtgg gcattccgaa gactccgatg tcgacttcga acccattcgg
     8041 tggcgcctgg acgtgtggaa cgcttcggcg caggtgtcca acgatgtcct ctgatcccgc
     8101 cgtgtcgtca aggccggcac tgctggtctt cgccgactcg ctggcctact acgggcccac
     8161 cgggggcctg cccgcggacg attcgcgtat ctggcctaat attgttgcgt cgcaattaga
     8221 ttgggatttg gaactggtcg gccgaatcgg ctggacctgc cgggacatct ggtgggcggc
     8281 gacccaggat ccacgggctt gggcggcgct gcccagggcg ggcgcggtga tcttcgcgac
     8341 cggcgggatg gattcgttgc cgtcggtgtt gccgaccgcg ctgcgcgagt tgatccgcta
     8401 cgtgcggcca ccgtggctgc ggcgctgggc acgcgatggc tatggttggt tgcagccgag
     8461 gctgtcgccg gtggcgaggt ctgccttgcc gccgcatcta agcgcggaat acctcgaact
     8521 gacgcgtggc gcaatcgatt tcaatcgccc cggcatcccg attgtggcgt cgctgccgtc
     8581 ggtgcacatc gccgagacat acggcaaggc ccaccagggc cgtgcgggga ccgtggccgc
     8641 gatcacggaa tgggcgcagg gccacaatgt tcccctggtg gatctcaaag ccgctgtggc
     8701 cgaacagatt atgagtggac gtggcaatcc tgacggcatt cactggaatt tcgaagccca
     8761 ccaggcggtc gcggaactga tgctcaaggc gctggccgaa gccggggttc cgaacgagaa
     8821 gccacgcggg tagcagcatg gcccgcgccg acgacgatgc agtgggggca ccgcccgctt
     8881 gcgggggacg atgcgatgag gtgggggcac gcccccacga gtgggtggta cccccacccg
     8941 tttgcggggg agagcggcgc tcgtgaccgt cgtggtggtg accgacgcgt cgtcgcggct
     9001 gccgggcgat ttgctggaaa agtggtcgat acgcgtcgtt ccgttgcaca tcctgctgga
     9061 cggccacgac ctgcgcgatg gcgtggacga catccccgag gacatctaca aacaacacgc
     9121 caccaccgcg gcggccaccc cggccgaact ggccgacacc taccagcagg cgttggccga
     9181 cagcggcggg gacggcgtgg tggcggtgca catttcgtcg gcgttgtcgg gaacctgcgg
     9241 cgccgcccaa cgaaccgccg ccgacctcga cccgagcgtg cgggtcatcg actcgaggtc
     9301 ggccgcgatg agcaccggct tcgtggcgct ggccgccgct cgggcagcgg cggcgggcgc
     9361 cgacctggac gccgtcacgg aggctgccag ttcggccgta tcccgcagcc atgcgttcgt
     9421 ggtggtgcac cggctggaca atctgcgccg cagcgggcgg atcggcgggg ccacggcctg
     9481 gctgggcacc gcgctggcac tcaaaccgct gttgcgtatc gacgacggca aactcgttct
     9541 ggcccaacgg gtgcgcaccg tcagccacgc gacggcggcg atgatcgatc gggtctgcga
     9601 ggtcgtcggt gagggctccg ccgccctggc ggtgcatcac gttgccaatc cggacggcgc
     9661 cgacgaggtg gcggcggcgc tggcggagcg gctaccgaac tgcgagccgg cgatcgtcac
     9721 cgcgttgggg ccggtgctcg ccctgcacgt cggcgccggg gccgtcgccg tctgtctcga
     9781 actggcggcg acctaacccg gcaatcagcg cgctaggcgc gcaccggtcg tcgggcgttg
     9841 tcaccgcgcg ggtgaacaac ctgcggccac cagaaccagc gccccagcaa cgtggcgatc
     9901 gacggcatca acaaggtgcg cacgacgagc gtgtcgagca gcagaccgat gcagacagtc
     9961 gagccgaact gacccagcac gcgcaaatca ctgccgagca tcgacgccat ggtgaacgcg
    10021 aacaccagac ccgctgccgt caccaccccg ccggtaccgg ccatcgaccg gatgatcccg
    10081 gtcttgattc cgccgtggag ctcttccttg aagcgggaga ccagcagcag gttgtagtcg
    10141 gacccgacgg ccaacaggat gatgacggac agcgccgcca cgatccagtg gatcttgatg
    10201 ccgaacagat cctgccaaat gagcacggac agcccgaatg acgcggcgat cgagctcgcc
    10261 gcggtgccta cgatcaccag cgcggccacc acgcttcggg tgagcagcag catgatcatg
    10321 aagatcaggg tcagcgccga taccaccgcg atcatgaggt cgtatttttc gccgtcggcc
    10381 atgtccttga aggtcgcggc ggtcccgccg agatagacct tggcgtccga cagcgaggat
    10441 tgcttgagtc cctcctgcgc ggccgtgcgc tcggaatcga cacgcgaaat gccttccggc
    10501 gtcatgggat cgccctggtg ggtgatgaag aagcgtgccg acttaccgtc cggcgacaag
    10561 aacatccgaa gccccgtctg gaagtccggg ttgtcgaacg cctccggcgg caggtagaag
    10621 aagtcgtcat tcttggaggc gtcgaagctt tgccccatga cgaccgccgt gttgctcatg
    10681 gcctcgatct gattgatcat cgccgagaac gtctggtaga gcgtcagcgt gatgccccgg
    10741 gtggtcttca gcgtcgcgat cagcgtgggg aacagcgtca tcagctcgtg cgtggccgag
    10801 gcggtgtgct ggatgtcagc cgtgaggtcg tggaacttct cggcgagctg gtcgatcccg
    10861 tcaaacgact caaatatgga tcgcaaagac cagcagatgg gaatatcgaa gcagtgcttg
    10921 tcccaataga agtaggcccg gatcggccgc cagaagtcgt cgaaatccgc gatgtggttc
    10981 cgcaaactat cggtgatatc ccgtgtttcc gccgtggtcc tggcgctgtc gtcagcagcc
    11041 ttggaaaggt cttgcgtcac cgcgtactgg cgttccgtga cgtcgatgtt gacctgcagc
    11101 tggtcggcca acttgaggat gtcggccatg cggtctttga ggaaggccat gttctgcatg
    11161 gttgtctggc tttgcatgct gttctggaac ggtatcgagc tgtgttgaat cggaatgccc
    11221 agcggccgag tgatgtcctg gaccatcgca atccccacgg tgcgcatcac atttttcgcc
    11281 acgcggtcca gcacgagcat gtcggcagga tttcgcatgt catggtcggc ctcgaccatc
    11341 aacatgtcgg gattcattcg cgcctgagag aaatgccggt ccgcggccgc ttgcccgaga
    11401 ttggacgggg ctgacgtcgg caggtaatag cgatcgttgt agctcgtctt gtagctcggc
    11461 agggcgacca tgccgaccag tacgatcgcg gaactcacgg ccagaaccgc tgcgggccaa
    11521 cgcaccacgg cggtgcccac ccgccgccac agccggcccc ccttggccgc ccgtttggac
    11581 tcgaacaggt ggaaccgact gcccacgaaa acaaccgcgg gacccagcgt gagtccggcc
    11641 aacaccacga ccagcatccc gatcgcgacg ggtgcgccca tcgtgttaaa ccagggcagc
    11701 cgggcgaagc tcaggcaata ggtcgctccg gcgatcgtca ggcccgaccc caacacgacc
    11761 ggagccaccc cgcggaatgt ggtgtagtag gcggtttctc gatcttcgcc ggcgcgcagt
    11821 gcctcttgat agcggccgat gaggaagatc ccgtagtccg tcccggccgc gatcgccaac
    11881 atggtcagga tgttggcggc gaacgtggtg agcccgaatg cattgtgata cgcgagaacc
    11941 gcgacgactc cccgcgaaca ccccagtgcg acgaaggtca tgaacagctg gaccagcgtg
    12001 gtgacgatgg agcggtagac cagcagcaac atgatcgcga tggcacccag ggtgaacagg
    12061 gtgatcttgg ccaggctcgc gttgccgatg aggtgcatgt catcgctgag cgcggcggga
    12121 ccggtgacgt aggccttcac cccgggcggt ggctggttgc ggtcgatcac cttgcgcacg
    12181 gcatccacgg aatcgttcgc ttgcgtcgtg ccttggttgc cggcgaggtt caacatgacg
    12241 taagcgccct tggcgtcggc gctctgtgcc ccggcagccg tcagccggtc tccccagaag
    12301 tcctggatgt gttggatgtg ctgcggatcc gcccgcaatt ggcgaaccag gctgtcgtag
    12361 tagcggtgcg cctcgtcgcc gaggggttgc tgaccctcca ggacgatcat cacggtgctg
    12421 ttggagtcga attcgtggaa gttgtgaccc agcagcatca tcgccttcat ggacggcgcg
    12481 tccagtggcg tcatcggcgc cgagtgcgct tcgccgacga tttccaaccg cgggacgatg
    12541 acgttcacca ggaccgtcac aaatacccag cccaggatga tgggcaccgc gaagatgcgg
    12601 attgtgtggg ggatgaccgg ccggtggcca cgctccggct tggcacggct cggggtcccg
    12661 atcgggccgg tgtcttggtc ttcggtggtc acatggctct tgttattcag atcgctcatg
    12721 cggacttcac caggcagaag gtctgggcgt tgtggccgtc ggagttttgc cggtcgcgca
    12781 cgacgccgtc cacggtgatc gtgcagctga ttcggctgcc gtcgctttgc gccatgatgt
    12841 tggcgctcac cgccggcagc gtggtcgaga tcgtggtcga ccacggcagc ggggccgcat
    12901 cgacctgatg tgtgttggcg ttctcgtccc agtagttgat gttcgcggtg gcacctggtg
    12961 ggccggacac ctcgtagacg acgacctttg ggttgaactg cacgatctcg atgccggcgc
    13021 cggcgttggc gttgaggtcc tgcgagccga agattttatg cagccgggtc acgaccaggg
    13081 ccgagacggc caggaccacg atcaacagca ggggtatcca tgcgcgtttg acgacgcgaa
    13141 ccatggcacc ggtgtttggt ctagccctca cctgaccgcc ttccgttcgc cgatctcgcc
    13201 tcgcaacgcc tctgggaacg gttgcttccc tcgtcatccc caccgatttc agctgaatct
    13261 tcgctcggct aagcatgtta atccttagtg cacgggagcg ttcctcgggg ccgttcaatg
    13321 tggccaacgg catagctggc cgcctttcta tcgggccacg tggccggtg
//
LOCUS       DASSGQ010000355         9940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_421434,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000355 DASSGQ010000000
VERSION     DASSGQ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9940
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421434"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..465)
                     /locus_tag="VFI55_15820"
     CDS             complement(88..465)
                     /locus_tag="VFI55_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016719164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET7075531.1"
                     /translation="MRWIVDGMNVIGSRPDGWWKDRGRAMVALVDSLDRWAQLHQDPV
                     TVVFERPPSTPVTSSVIEVAYAPRGAPNSADDEIVRRVRADARPHEIHVVTSDKGLTR
                     RVAGVGASVYPAAAFRDLIDPHG"
     gene            complement(909..2216)
                     /locus_tag="VFI55_15825"
     CDS             complement(909..2216)
                     /locus_tag="VFI55_15825"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HET7075532.1"
                     /translation="MACCDYPTVAEGCDGAFTVDASRVTFGRGCLAEVGDRATALGLR
                     RVALFSDAGVSRLPVFETAYESLRAAGVDVITYTHVHVEPTDASFLDGARFAQEWNPD
                     GYVSLGGGSVIDTCKAANLYATHPADLLTYVNAPIGEGKPIPGPLKPHLACPTTAGTG
                     SEVTGIAIFDYLSLSAKTGIASHALRPTEAVVDPDCTASLPAEVVAAAGLDVLSHALE
                     SYTARPYVHRRAPERPSARPMSQGANPWSDLGCREALSLLGQFLERAVRDAADTEARE
                     QMMWAATLAGIAFGNAGVHAPHGMAYAVAGLVRDFRPTGYPPDEPLVPHGMAVIVNAP
                     SAFRYTAEVNPVRHLEGARLLGADTRGADATDAGEVLAAELIRIMRAVGMPNGLSGVG
                     YTEDDVDALTEGAYPQQRLLQNSPREMTKPVLAGLFRQAMRYW"
     gene            2310..3431
                     /locus_tag="VFI55_15830"
     CDS             2310..3431
                     /locus_tag="VFI55_15830"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7075533.1"
                     /translation="MESCYGDVPLDDGIRSRRVDGVNGLSMHILEAGWHQPDRPVVLL
                     LHGFPELAFSWRKLMPLVARRGYHVIAPDQRGYGATTGWNADYDGDLTPFRLRHLVRD
                     VLALLSALRHQRVAAIVGHDLGSPVAAWCALLHPEIFGCVALLSAPFAGPPRPGPDPW
                     PRIDAALAALRPPRKHYQRYYATPQANSDMHTSAQGLSAFLRAYFHSKSHDWPHNHPH
                     ALKSWRAEDLAAMPHYYVMELDKGMAATVAEMAPSPEEVARCSWLTDAELDVYVRTFG
                     RNGFQGALNWYRNTFDAAAVAELQAFNGRRIEVPACFIAGRSDWGTYQTPGALEAMGS
                     TCAAFDGPSFIEEAGHWLQQEQPQRLDAELGEFLARHAL"
     gene            3499..4401
                     /locus_tag="VFI55_15835"
     CDS             3499..4401
                     /locus_tag="VFI55_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7075534.1"
                     /translation="MRSCKILVTGVTGQVAAPVAQALAVDNEVWGIARFANSAARETL
                     ENAGVRCHTVNLTTGDFAGLPADFDYVLNLAVAKSGNWDKDLRANAESVGLLMGYCRS
                     AKAFLHCSSAAVYDPPDDEPRTERAALGDNHRYLFPTYSISKIAGEVVARSTARILGV
                     STTIARLNVPYGDNGGWPYYHMEMMLAGIAIPVPPGGPARYNPIHEDDIIATIPKLLE
                     VASVPATTVNWCGDQTVSLQEWCGYLGSLVGAQPVFEESEQALRGNPTDPTRMHELIG
                     GTTVDWRDGMRRMAAKFHPELVGS"
     gene            4533..4976
                     /locus_tag="VFI55_15840"
     CDS             4533..4976
                     /locus_tag="VFI55_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7075535.1"
                     /translation="MAGRPEQHLRELALLRRVRDRIDRDYAQPLDVEALARGVNMSAG
                     HLSREFRRAYGESPYSYLMTRRIERAMALLRRGDLSVTEVCFAVGCSSLGTFSTRFTE
                     LVGMPPSTYRRQAADGTAGLPACVAKQVTRPIRNREAPVTGPYLA"
     gene            4981..5391
                     /locus_tag="VFI55_15845"
     CDS             4981..5391
                     /locus_tag="VFI55_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7075536.1"
                     /translation="MDITIHSSMLPSDDPEASLAFYRDILGFEVRLDVGKGKMRWLTI
                     GPPNQPDTSIVLSPPAVNPGITDDERRTIAEMMAKGTYAMLLLATKDLDGAFESLQAG
                     DAEIVQEPTEQPYGIRDCAVRDPAGNLIRIQQLR"
     gene            complement(5494..5748)
                     /locus_tag="VFI55_15850"
     CDS             complement(5494..5748)
                     /locus_tag="VFI55_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075537.1"
                     /translation="MKIVPTSIEEQINRVDRQRSLYIGISAGLVAVWSVFRLIWALYL
                     GITFGWFFGSMMVQLVLWGVIGAVAGVAAVGFLTRYSKGS"
     gene            complement(5880..7343)
                     /locus_tag="VFI55_15855"
     CDS             complement(5880..7343)
                     /locus_tag="VFI55_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET7075538.1"
                     /translation="MPNDVADPDAAVPQLRRDLSNRHIQLIAIGGAIGTGLFMGSGRT
                     ISHAGPAVLVVYAIIGFFVFFVLRAMGELLLSNLNYKSFVDFAADLLGPAAGFFMGWS
                     YWFAWIVTGIAEIVAITGYSKFWWPGLPAWLPVLVTVGLVLALNLSRVRNFGETEFWF
                     ALIKLAAIVCFIVAGAVLLATNFVSPQGDRATIENLWNDGGFFPTGFMGVVSGFQIAF
                     FAFVGVELVGSAAAETANPRRTLPRAINAVPLRVAIFYIGALLAILTVVPWRRFAGGE
                     SPFVMMFSLAGFAAAASIVNFVVITAAASSANSGVFSTGRMLFGLAEEGSAPAVFGRL
                     NRGGVPAVALLLTGVLLLTAIPLLYVGGSVMGAFTLVTTVSSLLFMFVWAMIVVSYLV
                     YRRRYPRRHAESTYKLPGGVVMCWAVLAFFAFVVWTLYTERETAVALAWFPMWFVALA
                     AGWLIVRRRPGRAERYRRFQAGMNDRIHEDTESVTAS"
     gene            7574..8257
                     /locus_tag="VFI55_15860"
     CDS             7574..8257
                     /locus_tag="VFI55_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075539.1"
                     /translation="MNAMSRLVLTPIAVLTMAVFLPIFMAVFPLAGSALADPAPTQVI
                     TTVPVGPNGQSISGYHEEPGQGNVVTVNDCTTPSPSAMAANIYYCSPSAAGAGTCWPS
                     TPGSLLCVDDPWDMRLHRVSYDGALPPVQPTATPDPFALVLDDGTRCLLRNGGAWGGR
                     ADGYVGVYGCGPHSNLAVLWLPSQGAGTCIDRSAPVWTVKVGPLSTPETPLPPPQTRA
                     VTAVWFAGD"
     gene            complement(8290..9138)
                     /locus_tag="VFI55_15865"
     CDS             complement(8290..9138)
                     /locus_tag="VFI55_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HET7075540.1"
                     /translation="MPEMDRRRMMLTTGIGVLAAALPMPEARAFPTPRDAPAGRMPPP
                     RAPSGQSGSYVFQDEFDGPAGSAPDSSKWTVATAREVIQDPTFWERPENIGQYRDDRR
                     NVFLDGNSNLVLRAAKDGPTYFSGKVQSTWRGGVGHTWEARIKLNCLTAGAWPAYWLG
                     NQDQGEIDVMEWYGNGNWPAATTVHAKANGGEWKTHNLALDSGWHTWRTQWDEAGIRF
                     WKDYADGAQPYFDVPASSLPDWPFNGPGYTVYPVFNLAVAGSGGGDPGPGTYPADMLV
                     DWIRVW"
     gene            9350..9595
                     /locus_tag="VFI55_15870"
     CDS             9350..9595
                     /locus_tag="VFI55_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075541.1"
                     /translation="MFQLLWLVLGGVEIVANTSEATVIFLQSHPAWIAAQRRAQERSE
                     AMHRHPSFRRHRIGAPSADVVPAVRNFKVYSSTDTPA"
BASE COUNT         1701 a         3419 c         3227 g         1593 t
ORIGIN      
        1 cggcgcctct cagcacccgt tccacatccg cgtgatgatg tcggcggccg gagggaggtc
       61 gtcgatgacg cgtcgacctt gtcagcatca gccgtgcggg tcgatcaggt cacggaaggc
      121 cgccgcggga tagacggagg cgcccacgcc ggcgacccgg cgggtcagcc ctttgtccga
      181 tgtcaccaca tgaatttcgt gcggccgggc gtcggctcgc acccgccgga cgatttcgtc
      241 gtcggccgaa ttcggcgcac ccctcggcgc atacgcaacc tcgatcactg acgaggtgac
      301 gggggttgac ggtggccgct cgaacaccac ggtcacgggg tcttggtgaa gctgcgccca
      361 tcggtcgagg ctgtcaacga gcgccaccat ggcgcggccg cggtccttcc accagccgtc
      421 cgggcgactc ccgatcacgt tcatgccgtc gacgatccag cgcaccaacc caccgtaggt
      481 cggccgtcat ctgcgctgaa ccggtggcgg aagtccgggc aaccaggcgg acagaccctc
      541 ctggataccc cggtggatct ggtcccgcac ggctgggctg tcggctggag gtgcctgtgg
      601 gcagctgcag ctcagggtgc cgaatgggtt cgagcccgcg cccggcgggc tcggctcggc
      661 tgcggccggt gtcgctgccg caacccagga gcccagcgcg atcgcaacgc cgatcactat
      721 tttcgtgatc agtgggcggt gccgtgtggt catgtcgtcc tctttctcga atttccgagc
      781 gcttgtcgag tacgacgttc cggcgatgcg attgggttca atgcgcgttc agactgctcc
      841 cgcagcgccg ccgccagccc gggtccggcc gcgctggacg tcggaccagt cctgggtgcg
      901 cgagtgtctc accaatagcg catggcttgc cgaaacaggc ccgccagaac cggtttggtc
      961 atctctcgcg ggctgttctg cagcaggcgc tgctgcgggt aagcaccctc cgtcagggcg
     1021 tccacgtcgt cctcggtgta gcccaccccg ctcagcccgt tcggcatgcc gaccgcccgc
     1081 atgatgcgga tgagttcggc ggcaagcacc tcgcccgcat ccgtcgcatc ggcgccccgc
     1141 gtatccgcgc cgagcaaccg cgcgccctcc aggtggcgta ccgggttgac ctccgcggtg
     1201 tagcgaaacg ccgagggcgc gttgacgatc acggccatgc cgtgcgggac cagcggctcg
     1261 tcgggcggat atcccgtcgg gcggaaatca cgtaccagcc cggccaccgc ataggccatg
     1321 ccatgcggcg cgtgtacccc cgcgttaccg aatgcgatgc cggcaagcgt tgcggcccac
     1381 atcatctgct cacgggcctc ggtgtcggcg gcgtcgcgca ccgcgcgctc caaaaactgc
     1441 cccagcaggc tcagcgcctc gcggcagccc aggtcgctcc acggattggc gccctgactc
     1501 atgggccgcg cgctggggcg ttcgggagcg cgccggtgca cgtagggccg cgcggtgtag
     1561 gactccagcg cgtgcgacag cacgtcgagc cccgccgcgg cgacaacttc ggcgggcaga
     1621 ctggcggtgc agtcggggtc gactactgcc tcggtgggac gcagcgcgtg agaagcgatg
     1681 cccgtctttg ccgacagcga cagatagtcg aagatcgcga tgccggtgac ctcgctgccg
     1741 gtgccggccg tggtggggca cgccagatgc ggcttgagcg gccccggtat cggtttgccc
     1801 tcgccgatcg gcgcgttgac gtaggtcagc aggtcggcgg gatgcgtggc gtacaggttg
     1861 gcggccttgc acgtgtcgat gaccgagccg ccgcccagcg acacatagcc gtcggggttc
     1921 cactcctgcg caaaacgcgc gccgtcgaga aatgacgcat cggtgggctc gacgtgaacg
     1981 tgtgtgtagg tgatcacgtc cacgccggcc gcgcggagcg actcgtacgc cgtctcgaac
     2041 accggcaatc tcgacacgcc ggcatcggag aacaacgcca cccggcgcag cccgagcgca
     2101 gtggccctgt cacccacctc cgccaggcag ccacgtccga aagtgacccg cgacgcgtcg
     2161 acggtgaatg cgccgtcgca gccctcagca acagttgggt aatcgcagca cgccatcggg
     2221 tcctcctcag ccgcttgcgt cggcaatcta tcgcacggcc ggctttcgtc ggaaaaggcc
     2281 ggggctgcgt cggtattgtt ggccgggtca tggagagttg ctacggcgat gtgcccctgg
     2341 acgacggcat tcgctcacgc cgcgtcgacg gcgtgaacgg tctatccatg cacatcctcg
     2401 aggcggggtg gcaccagccc gaccggccgg tggtgttgct gctgcacggc ttccccgagc
     2461 tggccttcag ctggcgcaag ctgatgcccc tcgtcgctag gcgcggctat cacgtgatcg
     2521 caccggacca acgcggttac ggtgcgacga ccggctggaa cgccgactac gacggcgacc
     2581 tgactccatt tcgcttgcgg cacttggtgc gtgacgtgct cgccttgctg tcggcactgc
     2641 ggcatcaacg cgtggccgcc atcgtcggcc atgatttggg ttcgccggtc gccgcgtggt
     2701 gcgccctgct ccatccagag atcttcggct gcgtggcgtt gttgagcgca ccgttcgccg
     2761 gaccaccacg cccgggcccc gatccctggc cgcggatcga cgccgccctg gccgccctgc
     2821 gaccaccacg caagcactac cagcgctatt acgctacgcc ccaggcgaat tccgacatgc
     2881 acaccagcgc ccagggcctc agcgctttcc tgcgcgccta ttttcacagc aaaagccacg
     2941 actggccgca caatcatccg cacgccctga agtcttggcg cgcagaggat ctggccgcca
     3001 tgccgcacta ctacgtgatg gaactcgaca agggcatggc ggccaccgtg gccgagatgg
     3061 caccctcgcc cgaagaagtc gcacgatgca gctggctcac cgacgcggag ctcgacgttt
     3121 atgtccgcac gttcggccgc aacggctttc agggtgccct caactggtac cgcaacacgt
     3181 ttgacgccgc cgcggtcgcc gaactacagg ccttcaacgg acgccgcatc gaggtgcccg
     3241 cgtgcttcat cgccggtcgc agcgactggg ggacctacca gactcccggc gcgctcgagg
     3301 ccatgggctc gacgtgcgcg gccttcgacg gaccatcctt catcgaggag gccggacatt
     3361 ggttgcagca ggagcaaccg cagcgtctcg acgccgagct gggagaattc ctcgcgcgcc
     3421 acgcgctgtg agggcgccgg ggcggggccg gcccgaaggc ggcgtcccgc ttggacaggc
     3481 gctcgctacc atcggcccat gcgcagctgc aagatcctcg tcaccggcgt aaccggccag
     3541 gtcgcggctc ccgtcgccca ggcgctggcg gtcgacaatg aggtctgggg tatcgcacgc
     3601 ttcgccaaca gcgcggcgcg ggagaccctc gagaacgcgg gggtgcgatg ccataccgtc
     3661 aacctcacaa ccggcgactt cgctggtctc cccgccgatt tcgactacgt cctcaacctg
     3721 gcggtggcca agagcggcaa ttgggataag gatctgcggg ccaacgcgga atcggtcggc
     3781 ctgctgatgg ggtactgccg cagcgcgaag gccttcctgc actgttcctc ggcggccgtc
     3841 tacgacccgc ccgacgacga gccgcggacc gagcgagccg cactgggcga taaccacagg
     3901 tatttgtttc ccacctattc catctccaag atcgccggcg aggtcgtcgc ccgctcgacg
     3961 gcgcgcattc tcggcgtgtc caccaccatc gcccgcctca acgtcccgta cggcgacaac
     4021 ggcgggtggc cgtactacca catggagatg atgctggccg gcatcgccat cccggtcccg
     4081 ccgggcggcc cggcccgcta caacccgatc cacgaggacg acatcatcgc caccatcccg
     4141 aagctgctcg aggtggcctc ggtcccggcg accacggtca actggtgtgg cgaccagacc
     4201 gtcagcctcc aggaatggtg tggctatctc ggttcgctcg tcggcgcaca gccggtgttc
     4261 gaggagagcg agcaagcgct gcgcggcaat cccaccgatc cgacccggat gcacgaactc
     4321 atcggcggca cgacggtcga ctggcgcgac gggatgcgcc gcatggcggc caagttccac
     4381 ccggagctgg tcggctccta gcggacgtcg cccagcgcgt tcaggctttc atggccactg
     4441 tggagcccct ggacagcgac aacgccccgc ttcccgtcgc ggaggggccg caatcctagg
     4501 caacccctgc cgaaggtggc accatcgcgc aggtggccgg tagacccgag cagcacctgc
     4561 gcgagctcgc gctgctgcgc cgcgtgcgcg accgcatcga ccgggactac gcgcagccgc
     4621 tggacgtcga ggcgctcgcc cgtggggtga acatgtcggc ggggcacctc agccgggagt
     4681 tccggcgcgc ctacggggag tcgccctatt cgtatctgat gacgcggcgc atcgagcgtg
     4741 cgatggcgct gctgcggcgc ggcgacctca gcgtcaccga ggtctgcttc gcggtcggtt
     4801 gctcgtcgct gggcacgttc agcacccggt tcaccgagct ggtagggatg ccacccagca
     4861 cgtaccggcg ccaggcagcg gacggcacgg cggggctacc ggcgtgcgtg gcgaaacagg
     4921 tgaccagacc gatcaggaat cgagaagcgc ccgtcaccgg gccctaccta gcctgagagc
     4981 atggacatca ccattcactc gagcatgctt ccgtccgacg atcccgaggc ctcgctggcc
     5041 ttctatcgcg acattttggg cttcgaggtt cgcctcgacg tcgggaaggg caagatgcgc
     5101 tggctcacaa tcggaccgcc gaaccagccc gacacatcga tcgtcttgag cccgccggcc
     5161 gtcaacccgg gcatcaccga cgacgagcgc cgcaccatcg ccgagatgat ggccaagggc
     5221 acctacgcca tgctgctgct ggccaccaag gatctcgacg gcgccttcga gagcctgcag
     5281 gccggcgatg ccgagatcgt ccaggagccc accgagcagc cgtacggaat tcgcgactgc
     5341 gccgtccgcg atcccgccgg caacctcatc cgcatccagc agctgcgctg agcgcccccg
     5401 gcacggtggc gaccggtccg ggtgccgaac ctccacatgg gaaagttccg aaccccggtc
     5461 gggttcggaa ctttccgtcg cggcgcaccc ggttcacgac cccttgctgt agcgggtcag
     5521 gaacccgacg gccgcgacac cggcgacggc accgatcacg ccccacagca ccagctgcac
     5581 catcatcgat ccaaagaacc aaccgaacgt gattcccagg tacagcgccc agatcagacg
     5641 gaacacactc cagaccgcaa ccaggcccgc gctgatcccg atgtagaggc tgcgttgccg
     5701 gtcgacgcga ttgatctgtt cttcgatgct ggtgggaacg atcttcattt ctccgacttc
     5761 ctttcatgac gcgaagcccg gttggcgtcg tcgttgcgcc aagtacaggc cgcgcggcgg
     5821 gctaccgatc ccaacaacgc gcgccgacgc gagggctcaa ccccgcggaa ccgagggcgt
     5881 caggaggcgg tcaccgactc ggtgtcctcg tggatgcggt cattcatgcc ggcctggaag
     5941 cggcggtacc tctcggcgcg gccgggccgg cgccggacga tcagccaacc cgcggcgagg
     6001 gccacgaacc acatcgggaa ccacgccaag gcgaccgcgg tttcccgttc ggtgtacagc
     6061 gtccagacca cgaacgcgaa gaaggcgagg accgcccagc acatcaccac gccaccgggc
     6121 agcttgtagg tcgactcggc gtgacgtcgc gggtaccggc ggcggtagac gaggtagctg
     6181 acgacgatca tcgcccacac gaacatgaac agcagcgagg aaacggtggt caccaacgtg
     6241 aaagcaccca tcaccgaacc gccgacgtag agcagcggaa tggccgtcag cagcaagaca
     6301 cccgtgagca gcagggcaac cgccggcacg ccaccgcggt tcagccggcc gaagaccgcc
     6361 ggggcgctgc cctcttctgc gaggccgaaa agcattcggc cggtggagaa gaccccggag
     6421 ttcgccgagg aggcggccgc ggtgatcacc acgaagttga cgatcgatgc ggcagccgca
     6481 aaccccgcca gggagaacat catcacgaac ggggactcgc cgccggcgaa ccgccgccac
     6541 ggcacgacgg tcaggattgc cagcagcgcg ccgatgtaga agatcgccac gcgcagcggg
     6601 acggcgttga tggcacgggg gagggtgcgg cgggggttgg ccgtctcggc cgccgcgctg
     6661 ccgacaagct ccacgccgac gaaggcgaag aaggcgatct ggaatccact caccactccc
     6721 atgaatcccg tcggaaagaa tccgccgtcg ttccacaggt tctcgatcgt cgcgcggtca
     6781 ccttgcggcg aaacgaagtt ggtcgccagc aggacggcgc cggcgacgat gaagcagacg
     6841 atcgcggcga gcttgatcaa ggcgaaccag aactcggtct ccccgaaatt gcggacccgg
     6901 gacaggttca acgcaagaac cagtccgacc gtgacgagga ccggcagcca ggccggcagg
     6961 ccgggccacc agaatttcga gtagccggtg atcgccacga tttccgcgat accggtgacg
     7021 atccaggcga accagtacga ccagcccatg aaaaaacccg ccgcggggcc caacagatcg
     7081 gccgcgaagt cgacgaacga cttgtaattc aggttggaca acagcagttc gcccatcgcc
     7141 cgcaacacga agaagacgaa gaacccgatg atcgcgtaca cgaccagtac cgccggcccg
     7201 gcgtgggaga tcgttcgccc ggaccccatg aacaggccgg tgccgatcgc gcctccgatt
     7261 gcgatcagct gaatatgccg gttggacagg tcccgacgca gttgcggaac agcggcatcg
     7321 gggtcggcaa catcgttcgg catgggtgat gtcctcgtaa tcaggaacgg gtgtaatcgg
     7381 gaacgggtgt caatcaggaa cggtttgtgt cctgcgctaa gcgttgccgc agccagcaac
     7441 atgatttcgc attttcaagc tccacgggtc acgtcggcgg caattctcta cacgcacgat
     7501 gtcgggcgat ctaccctttc ggaatagggg ccgctggatc gggctggatc gggctggata
     7561 ggaaggtggc tcgatgaacg cgatgtcgcg acttgtcctg acgcccatcg ccgtgctgac
     7621 gatggccgtt ttcctgccca ttttcatggc tgttttcccg ctggccgggt cggcactcgc
     7681 cgatccggca ccgacccagg tgatcacgac ggtgccagta gggcccaacg gccaatcgat
     7741 cagcggctac cacgaggaac ccggacaggg caacgtcgtc accgtcaacg actgcacgac
     7801 gccgtcgccg tcggcgatgg ccgccaatat ctactactgc tcgccctccg cggccggtgc
     7861 cggcacctgc tggccgtcga cgcccgggtc actgttgtgc gtggacgatc cctgggatat
     7921 gcgcctgcac cgggtgtcct atgacggcgc gctcccgccc gtgcagccga ccgccacgcc
     7981 ggacccgttt gcgctggttc tcgacgacgg cacccgttgc ctgctccgca acggcggcgc
     8041 ctggggcggc cgcgccgacg gctacgtcgg cgtctacgga tgcggcccgc actcgaacct
     8101 cgcggtgctg tggctgccca gccagggcgc cggaacctgc attgaccgct cggcgccggt
     8161 gtggacggtc aaggtcgggc cactgagcac accggagacc ccgcttccgc cgccccaaac
     8221 ccgcgcggtg acggccgtgt ggttcgccgg agactgagcc cgctgccggc tcagtgagcg
     8281 gtgttccgac taccagacgc ggatccagtc cacgagcatg tccgcgggat aggtgcccgg
     8341 cccggggtcg ccaccaccgg acccggccac cgcgaggttg aacaccgggt acaccgtgta
     8401 gcccgggccg ttgaacggcc agtccggtag ggagctcgcc ggcacgtcga agtacggttg
     8461 cgcgccgtca gcgtagtcct tccaaaagcg aattccggct tcgtcccact gggttcgcca
     8521 ggtgtgccac ccgctgtcca gcgcgaggtt gtgcgtcttc cattcaccgc cgttcgcctt
     8581 cgcgtggacc gtggtcgccg cgggccaatt cccgttgccg taccactcca ttacgtcgat
     8641 ttcgccctga tcctgatttc ccagccagta ggcgggccag gcgccggcgg tgaggcaatt
     8701 gagcttgatc cgcgcttccc aggtgtgccc gacaccgccc cgccacgtgc tttgaacctt
     8761 gccgctgaag taagtgggac cgtccttcgc ggcgcgcagg acgagattgg aattcccgtc
     8821 gagaaagacg ttgcggcgat cgtcgcggta ctgcccgatg ttctcgggcc gctcccagaa
     8881 cgtcggatcc tggatcacct cgcgggccgt cgcgacggtc cacttcgacg agtcgggggc
     8941 ggagcccgcc ggcccgtcga actcgtcctg aaaaacgtag gagccgctct ggccgctggg
     9001 cgccctgggc ggcggcattc gacccgcggg ggcatcccgt ggggtcggaa aagcccgagc
     9061 ctcgggcatc ggcagcgcgg ccgccagcac gccgatcccg gtcgtcagca tcatgcggcg
     9121 gcgatccatc tccggcataa gcaacgcacc ctagcagcca acgctgtacg gggtcgaaac
     9181 aggaattatg tcggtggccg tcgctagcgt gtggatcgcg gtcggcccga aacggacgac
     9241 ggcacggtag cggctcccga aaccggcgaa ttcacggcta attctcatct tccgggatgg
     9301 tttggtctca ggattggccg ggcattctgg aattacgaac cgataaccag tgttccagct
     9361 tttgtggctc gttttgggag gggttgaaat cgtggcgaat acatcggaag cgacggttat
     9421 tttccttcag tctcatccgg cttggatcgc ggctcagcgc cgcgcgcagg agcgctccga
     9481 agcgatgcac cgtcatccgt cttttcgccg acaccgcatc ggggcgccga gtgccgatgt
     9541 ggtccccgcg gtccgcaact tcaaggtcta ctccagcacc gacacgccgg cctgaacatt
     9601 tcgtcggatt tttccgggtt gcaaccggcc ccaataactt tttgaccgcg tggcactcgc
     9661 gttatcggca ctcaccggcg ttatgcaggt gacgccccat ttgaacacgc cgcattcgcc
     9721 ggcgtgttgg cgtcctcagt gatcggtttt ccgcggcggc gggggttgtg gctaaccgcc
     9781 ttgcgcggca atgtaatttc tcgggctgcg caatgacgag tacgtcggca taattctccg
     9841 ggtgccgcag acacgcagag cgaaggcgct cgccaaccca gcccagcccc acggtgcgct
     9901 tattcacgaa ttggagcgct acatcgaggc ccaccatccc
//
LOCUS       DASSGQ010000356         5185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_86791, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000356 DASSGQ010000000
VERSION     DASSGQ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86791"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VFI55_15875"
     CDS             complement(<1..417)
                     /locus_tag="VFI55_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HET7075542.1"
                     /translation="MQRRIMGIETEFGVTCTFHGHRRLSPDEVARYLFRRVVSWGRSS
                     NVFLRNGARLYLDVGSHPEYATAECDSLVQLVTHDRAGEWVLEDLLVDAEQRLADEGI
                     GGDIYLFKNNTDSAGNSYGCHENYLIVRAGEFSRISD"
     gene            complement(552..1334)
                     /gene="prcA"
                     /locus_tag="VFI55_15880"
     CDS             complement(552..1334)
                     /gene="prcA"
                     /locus_tag="VFI55_15880"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HET7075543.1"
                     /translation="MSFPYFISPEQAMRERSELARKGIARGRSVVALAYSGGVVFVAE
                     NPSRSLQKISELYDRVGFAAAGKYNEFDNLRRAGIQFADTRGYAYDRRDVTGRQLANV
                     YAQALGSIFTEQAKPYEVELCVAEVAHYGETRPPQLYRITYDGSIADEPHFVVMGGIT
                     EPITTALKESYAENADLRDALGIAVAALRAGSPDASNGDGNPLGVASLEVAILDASRP
                     RRAFRRINRTTLETLLRKRDATKSEDSASDGEASDSNGESSD"
     gene            complement(1331..2218)
                     /gene="prcB"
                     /locus_tag="VFI55_15885"
     CDS             complement(1331..2218)
                     /gene="prcB"
                     /locus_tag="VFI55_15885"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HET7075544.1"
                     /translation="MTWPFSDRLFTNSAFPGNPAVDLSSFADFLRRQAPELLPPSLSG
                     GAQPGGVGAQLPHGTTIVALKYPGGVVIAGDRRSTQGNMIAGRDVRKVYVTDDYTATG
                     IAGTAAIAVEFARLYAVELEHYEKLEGVPLTFAGKVNRLAIMVRGNLAAAMQGLVAVP
                     LLVAYDIHASDPQSAGRIVSFDAAGGWNLEEEGYQSVGSGSIFAKSSMKKLYSQVTDV
                     DSALRVVVEALYDAADDDSATGGPDLVRGIYPTAVTIDAEGAADVPEGRIAELAREVI
                     QSRSRADTFGPDAESRGEK"
     gene            complement(2215..2409)
                     /locus_tag="VFI55_15890"
     CDS             complement(2215..2409)
                     /locus_tag="VFI55_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HET7075545.1"
                     /translation="MAQEQTKRGGGGGDDDDFSDATAAGQERREKLSEETDDLLDEID
                     DVLEENAEDFVRAYVQKGGQ"
     gene            complement(2537..4045)
                     /gene="dop"
                     /locus_tag="VFI55_15895"
     CDS             complement(2537..4045)
                     /gene="dop"
                     /locus_tag="VFI55_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pup deamidase/depupylase"
                     /protein_id="HET7075546.1"
                     /translation="MQRIIGTEVEYGISSPSDPTANPILTSTQAVLAYAAAAGIQRAK
                     RTRWDYEVESPLRDARGFDLSRSAGPPPVVDADEVGAANMILTNGARLYVDHAHPEYS
                     APECTDPLDAVIWDKAGERVMEAAARHVASVPGAAKLQLYKNNVDGKGASYGSHENYL
                     MARQTPFSAIIAGLTPFLVSRQVVTGSGRVGIGPSGDEPGFQLSQRSDYIEVEVGLET
                     TLKRGIINTRDEPHADADRYRRLHVIIGDANLAETSTYLKLGTTALVLDLIEQGPAHG
                     IDLSDLMLARPVHAVHAISRDPSLRVAVALVDGRELTGLALQRIYLDRAAKLVDSRDP
                     DPRAADVVETWAHVLDQLERDPMDCAELLDWPAKLRLLEGFRQRENLSWSAPRLHLVD
                     LQYSDVRLDKGLYNRLVARGSMKRLVTEHQVLDAVDNPPTDTRAYFRGECLRRFGADI
                     AAASWDSVIFDLGGDSLVRIPTLEPLRGSKAHVGALLDSVDSAVELVEQLTS"
     gene            4115..>5185
                     /locus_tag="VFI55_15900"
     CDS             4115..>5185
                     /locus_tag="VFI55_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075547.1"
                     /translation="MDPSPRRGPDGPAARTAGLRKTGSSAAERWFLERGLPSVLTRRA
                     RWRRLWPRSAPILAAYGTLQACVLSVYLITGGHDVEITGEPTTSELIVLAIVGVTLPM
                     MAVVGWLVSRIQGGGTRAAIATLAVVVVACVGTTVGGLAELVQEAIVVAAVLVLTGCG
                     AGAVVGWALRMMLSHLATVGALVVRALPVVLLTALVFFNGYVWIMAATISGDRLGLAM
                     AFLVSIAAAFVISATRERVRPMLRSPTTALPNDPKNSERLADTPFANMPDASDDVPLK
                     RAERLNLVFLLATSQLAQILVVAVVTATIYLMLGLIVLTPSLLNEWTHTYKSTATVLG
                     FTLPVPDSLIRMALFLGALTFMY"
BASE COUNT          803 a         1739 c         1731 g          912 t
ORIGIN      
        1 atcggagatc cgggagaact cgccggcgcg cacgatcaga tagttctcgt ggcagccata
       61 cgagttgccc gccgagtcgg tgttgttctt gaacaagtag atgtcgccgc cgatgccctc
      121 atcggcgagc cgctgctcgg cgtcgacgag cagatcctcc agcacccatt caccggcgcg
      181 gtcgtgggtg accagctgca ccaggctgtc gcattcggcg gtggcgtatt ccgggtggct
      241 gcccacgtcg aggtagaggc gcgcgccgtt gcgcaagaag acattggagc tgcggcccca
      301 cgacaccacc cgccggaaca agtagcgggc cacctcgtcc ggagagaggc ggcgatggcc
      361 gtggaacgtg caggtgacac cgaactcggt ctcgatgccc ataattcgcc gctgcacgta
      421 atcgagggta ctggttgtct gaccgctaag gtgagcaagc cgcgccctgg gatggtatta
      481 ggggcagcgg gtcgtccgcg ctcggcctcc accgccgagc ggtgggttgc gggttctctg
      541 gcggggcggg tctagtccga ggattcgccg ttcgaatccg acgcttcgcc gtctgaggct
      601 gagtcctcgg atttcgtcgc atcccgcttc cgcagcaagg tttccaaggt ggtgcgattg
      661 atccgccgaa aggcccgccg cggccggctg gcgtccagaa tggccacctc gaggctggcc
      721 acaccgaggg ggttcccgtc gccgttcgag gcgtccgggc tacccgcccg cagcgccgcc
      781 accgcgatcc ccaacgcgtc acgcaggtcg gcgttctcgg cgtacgactc cttgagcgcg
      841 gtggtgatcg gctcggtgat cccgcccatg accacgaagt gcggctcgtc ggcaatcgac
      901 ccgtcatagg tgatccggta cagctggggc ggtctggtct cgccgtagtg cgcgacttcg
      961 gcgacgcaca actcgacctc gtacggtttg gcctgctcgg tgaagatgct gcccagcgcc
     1021 tgcgcgtaga cgttagccag ttgccggccg gtgacgtcgc ggcgatcgta ggcgtagccg
     1081 cgggtgtcgg cgaactggat gcccgcgcgg cgcaagttgt cgaactcgtt gtacttgccc
     1141 gccgcggcaa agcccacgcg atcgtagagc tcgctgatct tctgcagcga ccgcgacggg
     1201 ttctccgcga cgaacaccac accaccggag taggccagcg cgaccacgct gcggccccgc
     1261 gcgatgcctt tgcgtgcgag ctcgctgcgc tcgcgcatcg cctgctcggg cgagatgaaa
     1321 tacggaaagc tcacttctca ccccgggact cggcgtcggg gccgaaagtg tccgcccgcg
     1381 aacggctttg gatgacctct cgagccagtt cggcgatgcg accctccggc acgtcagccg
     1441 caccttcggc gtcgatggtg accgccgtgg gatagatgcc gcggaccagg tccggcccgc
     1501 cggtcgcgga atcgtcgtcg gcggcgtcgt agagcgcctc cacgactacc cgcagcgcgg
     1561 aatcgacgtc agtgacttgc gaatacaact tcttcatcga cgacttggcg aagatcgagc
     1621 ctgaccccac cgactggtag ccctcttcct cgaggttcca gccgccggcg gcgtcgaacg
     1681 agacgatgcg gccggcgctt tgggggtccg acgcgtggat gtcatacgcc accagcagcg
     1741 gcacagccac cagcccctgc attgccgccg cgaggtttcc gcgcaccatg atcgcgagcc
     1801 gattgacctt gccggcaaac gtgagcggga caccttcgag cttctcgtag tgctcgagtt
     1861 ccacggcata cagacgggcg aactccacgg cgatcgcggc ggtgcccgca atgcccgtcg
     1921 cggtgtaatc gtcggtgacg tacaccttgc gcacatcgcg cccggcgatc atgttgccct
     1981 gcgtcgagcg ccggtcaccg gcgatgacga caccaccggg gtacttcaac gcgacgatgg
     2041 tggtgccatg cggcagctgc gctccgacac caccgggttg cgcaccgccg ctgagacttg
     2101 gcggcagcaa ttccggcgcc tgccggcgca ggaagtcagc gaacgaggac aggtctacag
     2161 caggattccc gggaaatgcg gagttagtga acaggcgatc agagaacggc caggtcactg
     2221 tccgcccttt tggacatacg cacggacgaa gtcctcggcg ttctcctcaa ggacgtcgtc
     2281 aatctcgtcg agcagatcgt cggtctcctc ggacaacttc tcgcgacgct cctggcccgc
     2341 ggccgtggcg tcgctgaagt cgtcgtcgtc gccgcctcca ccaccacgct tcgtctgctc
     2401 ctgcgccatc gccgcctcct gctcggtcac cggctcattg ccgacttttg ccggctttct
     2461 cgggggctgc gccgtcgcgc ccgccggtat gtctctaccc taccggtcaa ccccgacgtt
     2521 tcccgaccta acgggcttag ctagttagtt gttctaccag ctcgacggcg ctgtccaccg
     2581 agtccagcag cgctcccacg tgcgctttac tgccccgcag cggctccagc gtcgggattc
     2641 gtaccagtga atcgccaccg agatcgaaaa tcaccgagtc ccagctggcc gcggcgatat
     2701 cggccccgaa tctgcgcaga cattcgccgc ggaagtacgc gcgggtatcc gtcggcgggt
     2761 tgtcgaccgc gtcgagcacc tggtgttcgg tgaccagacg cttcatcgag ccgcgggcga
     2821 ccagccggtt gtagaggccc ttgtccagcc ggacgtcgga atactgcagg tcgaccaggt
     2881 gcaaccgggg cgccgaccag ctcaggttct cccgctgccg gaacccctcg agcagtcgca
     2941 gcttggcggg ccagtccagc agctcagcgc agtccatcgg gtcgcgctcg agctggtcga
     3001 gcacgtgtgc ccaggtctcg acgacgtccg ccgcgcgcgg atcagggtcg cggctgtcga
     3061 ccagcttggc cgcccggtcg agatagatcc gttgcagcgc aaggccggtc agttcgcggc
     3121 cgtccaccag ggccacggcg acccgcagcg acgggtcgcg gctgatggca tggaccgcat
     3181 gcaccggccg cgccagcatc aggtcggaca ggtctatccc gtgcgcgggg ccttgctcga
     3241 tcaaatcgag caccagcgcc gtggtgccca gtttgaggta ggtcgacgtc tcggccaggt
     3301 tggcatcgcc gatgatcacg tgcagccggc ggtaccggtc ggcgtcggcg tgcggttcgt
     3361 cgcgggtgtt gatgatgccg cgcttcagcg tggtctccag cccgacctcg acctcgatgt
     3421 aatcagaacg ctgggacagc tggaagccgg gctcgtcgcc ggagggcccg atgccgaccc
     3481 ggcccgagcc ggtcaccacc tggcgggaca ccagaaacgg ggtgagcccg gcgatgatcg
     3541 ccgagaacgg cgtctggcga gccatcaggt agttctcgtg cgacccgtag gacgcgcctt
     3601 tgccgtcgac gttgttcttg tagagctgca gcttcgcggc tccgggcaca ctggcgacgt
     3661 gccgggcggc ggcctccatc acccgttcgc cggccttgtc ccagatcacc gcgtccagcg
     3721 ggtcggtgca ctcgggcgcg gagtattcgg ggtgcgcgtg gtcgacgtag agccgtgccc
     3781 cgttggtcaa gatcatgttg gcggcgccga cctcgtcggc gtcgacgacg ggcggcggtc
     3841 cggccgaacg gctcaggtcg aagccgcggg cgtcccgcaa cggcgattcc acctcatagt
     3901 cccagcgggt tcgcttcgcg cgctgaatcc cggcggccgc cgcgtaggcc aagactgcct
     3961 gggtcgaggt gaggatcggg ttggcggtcg ggtcggacgg cgacgagatg ccgtattcga
     4021 cctccgtccc gataatccgt tgcatccgtt aagcctaggc cgggcgacga tgcgccccgc
     4081 gcagcggggc gatccgccgc tagggtgagc cggcatggac ccatccccgc gccgcgggcc
     4141 ggacggccca gcagcgcgca ccgccgggtt gcgcaagacg gggagcagcg cggccgaacg
     4201 ctggtttcta gagcgcggtt tgccgtccgt gctgaccagg cgggcccgat ggcgacggct
     4261 ctggccgcgg tcggccccca tcctcgccgc gtacggaaca ctgcaggcct gtgtcctgtc
     4321 ggtctacttg atcaccggcg gccacgacgt cgagatcacc ggggagccga caacctccga
     4381 attgatcgtg ctggcgatcg tcggggtgac cctcccaatg atggccgtcg tcggctggct
     4441 ggtgtcgcgg atccaaggcg gtggcacccg tgccgctata gcgaccctgg cggtggtcgt
     4501 cgtggcatgc gtcggaacca ccgtcggtgg actcgcggaa ttggtgcagg aagccatcgt
     4561 cgtggccgcg gtgctggtgc tgaccgggtg cggcgccggc gcggtcgtcg gatgggcgct
     4621 acggatgatg ctgtcgcacc tcgcgaccgt gggagcactg gtggtccggg cattgccggt
     4681 cgtcctgctg accgccttgg tgttcttcaa cggctacgtc tggatcatgg ccgcgacgat
     4741 cagcggagac cggctggggc tggcaatggc atttctggtc tccatcgccg cggcgttcgt
     4801 catctccgcc acccgagaac gggttaggcc gatgctgcgg tcgccgacga cggcgctgcc
     4861 gaatgacccc aaaaactctg agcggctcgc cgacacgccc ttcgcgaaca tgccggatgc
     4921 ttctgatgac gtgccgctga aaagggccga acgcctcaac ctcgtcttcc tgctggccac
     4981 ctcacaactg gcacagatcc tggtggtggc agtcgtcacc gcgacgatct acctgatgct
     5041 tgggctgatc gttctcactc catcgctgct caacgaatgg acccacacat acaagagcac
     5101 ggcgacggtg ttgggattca cgctgccggt gccggattcg ctgatccgta tggcgctctt
     5161 cctcggcgcg ttgacgttca tgtat
//
LOCUS       DASSGQ010000357         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1425073,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000357 DASSGQ010000000
VERSION     DASSGQ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425073"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VFI55_15905"
     CDS             complement(<1..880)
                     /locus_tag="VFI55_15905"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET7075548.1"
                     /translation="MGGAVERQPRSHEVTRGYERAPARAMLRAVGMTDEDFVKPQIGV
                     ASSWNEITPCNLSLDRLAKRAKDGVRAAGGFPLEFATISVSDGISMGHEGMRASLVSR
                     EVIADSVECVMHAERFDGAVLLAGCDKSEPGMLMAAARLDLAAVFLYAGSILPGRLGD
                     RDLTIVDVFEGVGACALGKITREELDAIERAACPGEGACAGMFTANTMASAAEALGMS
                     LPGSAAPPATDRRRDGYARRSGEAVVGLLRRGITARDILTKEAFENAIAVVMAFGGST
                     NAVLHLLAIAHEADVKL"
     gene            complement(997..2133)
                     /locus_tag="VFI55_15910"
     CDS             complement(997..2133)
                     /locus_tag="VFI55_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075549.1"
                     /translation="MRRPLLLAAVLLLLASGAPPAAAALPASSHARVRIPVKTANASQ
                     AVTPVQIYGAWHCGNDYCTWGAVRSIAEFDSQNHWLIDRGDGRPSVNMVILSFVNPLS
                     LLHQTTDAATLNGVPRGMTPEIVNYFTSRGVRVMLSIGGITYTDDWDAALAENPTLFG
                     QRAAAVASQLGVGIEIDYEQNTDPNLTALQAFIDAYRAINPYDATGTRPQARLTIDVA
                     AGDRWLIALNRKATADWLRTDTPVLDYANAMVPARPASATSAQASWQEHVDGKPQYSP
                     PVPPLAPAKFTGGIYIAYGGKALPECIDYNTSVQRATAPYVQTVVPNGAGTTAGMLGF
                     MFWAAERPSTRGIGTVPPHTCEGGVGAGATALTIPVPMPALRQS"
     gene            complement(2246..>2686)
                     /locus_tag="VFI55_15915"
     CDS             complement(2246..>2686)
                     /locus_tag="VFI55_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7075550.1"
                     /translation="RDHPDIGVLVLSQYIETRYAAQLLAFNAAGVGYLLKDRVADVAD
                     FVDALTRVATGGTALDPDVVSQLLGAHQRARGLAELTPREREVLALMAEGRSNTGIAA
                     ALTVTGGVVEKHVAGIFSKLGLPQAEGDNRRVLAVLRYLGSPPT"
BASE COUNT          411 a          880 c          948 g          447 t
ORIGIN      
        1 acagcttcac gtcggcctcg tgtgcgatgg ccagcaggtg caggacggca ttggtcgagc
       61 cgccgaatgc catcaccacc gcgatcgcgt tctcgaacgc ctccttcgtg aggatgtcgc
      121 gggcggtgat gccgcgccgc aacagaccga cgaccgcttc accgctgcgg cgtgcgtatc
      181 cgtcgcgccg acggtcggtg gccggcggtg cggcgctgcc gggcagcgac atgcccagcg
      241 cctcggccgc gctggccatg gtgttggcgg tgaacatgcc cgcgcaggcg ccctcgcccg
      301 ggcaggccgc gcgttcgatc gcgtccaact cctcgcgcgt gatctttccg agtgcgcagg
      361 cgccgacgcc ctcgaacacg tcgacgatgg tcaggtcccg gtccccgagc cgaccgggaa
      421 ggattgatcc cgcgtacagg aataccgcgg ccaggtccag gcgcgcggcg gccatcagca
      481 tgcccggctc ggacttgtcg cacccggcga gcaggacagc gccgtcgaat cgttcggcgt
      541 gcataacgca ctccaccgaa tccgcgatca cctcacggct caccagcgac gcccgcatcc
      601 cctcgtggcc catcgagatg ccgtccgaca ccgagatcgt ggcgaactcc agcggaaacc
      661 cgccggccgc gcgtaccccg tccttggctc gcttcgcgag ccggtccagc gacaggttgc
      721 acggcgtgat ctcgttccac gacgaggcca ccccgatctg cggcttgacg aaatcctcgt
      781 cggtcatacc gaccgcccga agcatcgcac gcgccggggc acgctcatac ccgcgagtga
      841 cctcatgact gcgcggctgg cgttccacag ctccacccat agccggacct tacgacgccc
      901 gccgctacga tgcgacgccg ccacgatcca ggatcgtcac gtccttcggg gcatggcgac
      961 gcggcccccg attcgccggt tgttcgctat tcgatgtcag ctctggcgga gtgccggcat
     1021 cggcaccggg atggtcagcg cggtggctcc cgcaccgaca ccgccttcgc aggtgtgcgg
     1081 aggcacggtt ccgatgcccc gggtagaggg tcgctcggcg gcccagaaca tgaagccgag
     1141 catgccggct gtcgtaccgg caccgttggg cacaacagtc tgcacgtaag gggcggtggc
     1201 ccgctgtacc gaggtgttgt agtcgatgca ttcgggtagg gctttgccgc cgtacgcgat
     1261 gtagatcccg ccggtgaatt tggctggggc cagcggtggg acaggcgggc tgtactgcgg
     1321 cttgccgtcg acgtgctcct gccagctcgc ctgtgcgctg gtcgccgagg cgggccgggc
     1381 gggcaccatg gcgttggcgt agtcgagtac cggggtgtcg gtacgcagcc agtcagcggt
     1441 cgccttgcgg ttcagcgcaa tgagccaccg gtcgccggcc gcgacatcga tggtgagccg
     1501 ggcctggggt cgggtaccgg tggcgtcata cgggttgatg gcgcggtagg cgtcgatgaa
     1561 tgcctgcagg gcggtgaggt tcgggtcggt attctgttca tagtcgatct cgatgccgac
     1621 gccgagctgg ctggccacgg cggcggcccg ctgcccgaac agcgtcgggt tctcggcgag
     1681 cgcggcgtcc cagtcgtcgg tgtaggtgat accaccgatg gagagcatca cccggacgcc
     1741 gcgcgaggtg aagtagttga cgatctcggg ggtcatgccg cgcggtacgc cgttgagcgt
     1801 cgcagcgtcg gtggtctggt gcagcagcga aagcgggttg acgaagctga ggatgaccat
     1861 gttgacggac ggtcgaccgt cacctcggtc gattagccag tggttctggc tgtcgaactc
     1921 ggcgatgctg cggaccgcac cccaggtgca atagtcattg ccgcagtgcc aggcgccgta
     1981 gatctgcacg ggggtgacgg cctggctcgc gttggcggtc ttgaccggaa tgcgtacgcg
     2041 ggcatgcgag ctggccggga gagcggctgc ggcgggtgga gcgcctgatg ccagtaggag
     2101 cagaaccgcc gccagcagta ggggccgtcg catggtgacc tcccgggcag atcagtgcac
     2161 cttgtttaca gtaggttctc acatgcgtca atattctgtc cgggtccaga gccgggcacc
     2221 gcgcagatcc actgcgccgg acgggtcacg tgggtgggga tccgaggtag cgcagaaccg
     2281 cgagcactcg ccggttgtcg ccttcggctt gcggcaggcc caacttgctg aagatgccgg
     2341 ccacgtgctt ctcgacgacc ccaccggtaa cggtgagggc ggccgcgatc cctgtgttgg
     2401 aacgcccttc agccatcagg gccagcacct cgcgttcccg cggcgtcagc tcggcgaggc
     2461 cacgcgcgcg ttgatgggcg cccagcagtt ggctgaccac gtccggatca agcgcggtac
     2521 cgccagtggc gacccgggtc agcgcgtcga cgaagtcggc cacgtcggcg acgcggtcct
     2581 tgagtagata tccgacgccg gcggcgttga aggccagcag ctgggcggcg taccgggtct
     2641 cgatgtactg agatagcacg aggaccccga tgtccgggtg gtcgcg
//
LOCUS       DASSGQ010000358         3484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_391670,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000358 DASSGQ010000000
VERSION     DASSGQ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391670"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VFI55_15920"
     CDS             complement(<1..449)
                     /locus_tag="VFI55_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7075551.1"
                     /translation="MTAAGLACGACGIELPPNSKFCNECGSPVAVPGTTAEYKQVTVL
                     FADVVRSMDLAAALGAERLREIMTTLVERSAAVVQRYGGTVDKFTGDGIMAVFGAPIA
                     LEDHAIRACWAATDIQKETERLAAEVERSDGTTVKLRIGLNSGQVIVG"
     gene            645..965
                     /locus_tag="VFI55_15925"
     CDS             645..965
                     /locus_tag="VFI55_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075552.1"
                     /translation="MLEVQVGTVEVDVPRSIGYFGGLVVATGLGLIEPPLAQFIAAVP
                     VFKVLTNTALPKAVRIVVEVLEGAAKPVGSDAEGVIALEDQQQSDTAVVHVPALRKRS
                     ASRR"
     gene            complement(962..3334)
                     /locus_tag="VFI55_15930"
     CDS             complement(962..3334)
                     /locus_tag="VFI55_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HET7075553.1"
                     /translation="MADEKSAAQALEAPAEPSLVAEPVAETPEPPVETLKTTSSASRR
                     VRARLARRMMAQRGTLNPVLEPLVAVHREIYPKANLSMLQRAFEVADQRHATQLRHSG
                     DPYITHPLAVANILAELGMDTTTLVAALLHDTVEDTGYTLEALSEEFGEEVGHLVDGV
                     TKLDRVELGNAAEGETIRKMITAMARDPRVLVIKVADRLHNMRTMRFLPPEKQARKSR
                     ETLEVIAPLAHRLGMASVKWELEDLSFAILHPKKYEEIVRLVAGRAPSRDTYLAKVRA
                     EITNTLNASKIKATVEGRPKHYWSIYQKMIVKGRDFDDIHDLVGVRILCDEIRDCYAA
                     VGVVHSLWQPMAGRFKDYIAQPRYSVYQSLHTTVVGPEGKPLEIQIRTRDMHRTAEYG
                     IAAHWRYKEAKGRNGVPHPHAAAEIDDMAWMRQLLDWQREAADPGEFLESLRYDLAVQ
                     ELFVFTPKGDVITLPSGSTPVDFAYAVHTEVGHRCIGARVNGRLVALERKLENGEVVE
                     VFTSKAPNAGPSRDWQQFVVSPRAKTKIRQWFAKERREEALEAGKDAMAREVRRGGLP
                     LQRLVNGESMASVARELHYTDVSALYTAIGEGHVSARHVVQRLLAELGGIDQAEEELA
                     ERSTPTTMLRRPRSNDDVGVSVPGAPGVLTKLAKCCTPVPGDQIMGFITRGGGVSVHR
                     TDCTNATSLQQQSERIIEVLWAPSASSVFLVAIQVEALDRHRLLSDITRVLADEKVNI
                     LSASVTTSGDRVAISRFTFEMGDPKHLGHLLNVVRNVEGVYDVYRVTSAA"
BASE COUNT          555 a         1162 c         1158 g          609 t
ORIGIN      
        1 ccgacgatga cctggcccga gttcagccca atccgcagct tcaccgtggt tccgtcagac
       61 cgctcgactt cggccgcgag ccgctcggtc tccttctgga tgtccgtcgc ggcccagcac
      121 gcgcggattg cgtggtcctc caaggcgatt ggcgccccga aaaccgccat gatgccgtca
      181 ccggtgaatt tgtcgacagt accgccgtag cgctggacga cagcggccga acgctcgacc
      241 agtgttgtca tgatttcgcg cagccgctct gcgcccaacg ccgctgccag atccatcgag
      301 cgcaccacgt cggcgaacag caccgtcacc tgcttgtact ccgccgttgt gccggggacg
      361 gcgaccggcg acccacactc gttgcaaaac ttcgaattcg gcggcaattc gataccgcac
      421 gcaccgcaag ccaatcccgc cgccgtcatg ccagtaccac ctatcgcggt caaaccccga
      481 tccgccaaga ataggggcgg ttggcggtga acaacacgct caattccgtg cgagtgggct
      541 aggcgcgacc gcacggagta cccgaagact tttcggcatg agtatcagga gggcgtcatg
      601 gccaccagta gcgccgtgag atccggttcg gcgcaccgga aaacgtgttg gaagtccagg
      661 tgggcaccgt cgaagtcgac gtgccaaggt cgatcggcta tttcggcggc ctggtggtgg
      721 ccaccgggct gggcctgatc gagccgccgc tggcgcagtt catcgcggcg gtcccggtct
      781 tcaaggtgtt gacgaacacc gcgctgccca aggcggttcg catcgtcgtt gaggtgctcg
      841 agggggcggc caaaccggtc ggcagcgacg cggaaggcgt catcgcgttg gaggaccagc
      901 aacagtccga caccgcggtc gtgcacgtgc cggcgctgcg caagcggtcg gccagccggc
      961 gttaagcggc ggatgtcacc cggtacacgt cgtagacgcc ttcgacgttg cgcacgacgt
     1021 tgagcagatg cccgagatgc ttcgggtcgc ccatctcgaa ggtgaagcgg ctgatcgcca
     1081 cccggtcacc ggaggtggtg accgacgccg acaggatgtt gaccttctcg tcggccagca
     1141 cccgcgtgat gtccgagagc aacctgtgcc ggtcgagcgc ctcgacctga atggccacca
     1201 gaaacaccga ggacgccgac ggtgcccaca gcacctcgat gatgcgctct gactgctgtt
     1261 gcagcgacgt cgcgttggtg cagtcggtgc ggtgcacact gaccccgccg ccgcgggtga
     1321 tgaaccccat gatctgatcg ccgggcacgg gcgtacagca cttggccagc ttcgtcagca
     1381 ctccgggcgc gcccgggacc gagaccccga cgtcgtcgtt gctgcggggg cggcgcagca
     1441 tggtcgtcgg cgtggaacgc tcggccagct cctcctcggc ctggtcgatg ccgccgagct
     1501 cggccagcaa ccgctgcacg acgtgccggg cggacacgtg cccctcaccg atcgcggtgt
     1561 agagcgcgga cacgtccgtg tagtgcagct cacgggcgac ggaggccatg gattcgccat
     1621 tgaccaagcg ctgcaacgga agtccgccgc ggcgtacctc gcgcgccatg gcgtccttgc
     1681 cggcctcgag ggcttcttcc cgccgttctt tggcgaacca ctgccggatc ttggtcttgg
     1741 cgcgcggcga caccacgaac tgctgccagt cccgcgacgg gccggcattg ggcgccttgg
     1801 acgtgaagac ctcgacgact tcgccgtttt ccagcttgcg ttccagcgcc accaggcgcc
     1861 cgttgacccg cgcgccgatg cagcggtggc cgacctcggt gtggacggcg taggcgaagt
     1921 ccaccggtgt cgacccggac ggcagggtga tcacgtcgcc cttgggggtg aacacgaaaa
     1981 gctcttgcac cgcaaggtcg taacgcaacg actccaggaa ctcgccgggg tcggcggcct
     2041 cccgctgcca gtcgagcagc tggcgcatcc aggccatgtc gtcgatctcg gcggcggcgt
     2101 gtggatgcgg aacgccgttg cggcccttgg cttccttata gcgccagtgc gcggcgatgc
     2161 cgtattcggc ggtgcggtgc atgtcgcggg tgcggatctg gatctccagc ggtttgccct
     2221 ccgggccgac gacggtggtg tgcagcgact ggtacacgct gtagcgcggt tgggcgatat
     2281 agtccttgaa ccgcccggcc atcggctgcc acagcgaatg caccacgccg acagcggcgt
     2341 agcagtcccg gatctcgtcg cacaggatgc ggacgccgac caggtcgtgg atgtcgtcga
     2401 agtcgcggcc cttgacgatc atcttctggt agatcgacca gtagtgcttg gggcggccct
     2461 ccacggtcgc cttgatcttc gacgcgttca gggtgttggt gatctccgcg cggaccttgg
     2521 ccaggtaggt gtcccgggac ggcgcgcgac cggcgaccag ccgcacgatc tcctcgtact
     2581 tcttggggtg caggatcgcg aacgacaggt cttccagctc ccacttgacg ctggccatgc
     2641 ccagccgatg tgccagcggc gcaatgactt ccagcgtctc gcgggacttg cgcgcctgct
     2701 tttccggggg caggaagcgc atggtgcgca tgttgtgcag gcgatcggcg accttgatca
     2761 ccagcacccg cgggtcgcgc gccatggcgg tgatcatctt gcggatggtc tcgccctccg
     2821 cggcgttgcc cagctcgacc cgatccagct tggtcacgcc gtcgacgagg tggcccacct
     2881 cttcgccgaa ttcctcgctc aacgcctcga gcgtgtagcc ggtgtcttcg acggtgtcgt
     2941 gcagcaacgc ggccaccagg gtggtggtgt ccatgcccag ttcggccagg atgttggcga
     3001 ccgccagcgg gtgggtgatg tacggatcac cggaatggcg cagctgagtg gcgtggcgct
     3061 ggtcggcgac ctcaaaggcc cgctgcagca tcgacaggtt cgccttggga tagatctccc
     3121 ggtgaacggc caccagtggc tcgagcacgg gattgagggt gccgcgctgg gccatcatcc
     3181 gccgggccag ccgggcccgg acccgacgag acgcactgct ggtcgtcttc agggtctcga
     3241 ccggcggctc cggcgtctcg gcgaccggct ccgcaacgag cgagggttcc gccggcgcct
     3301 caagagcttg tgccgcgctc ttctcgtccg ccacgttggt cacctccgac ctcgaggata
     3361 tcgccaaagt gaggcaaggc cccgctcctc agcagaccgt tcgttcctct cccgatgggc
     3421 gcaagccgtc gcgcgaccga ccaataggag ggtttcattt gtgatagcgc cgttgatagc
     3481 acaa
//
LOCUS       DASSGQ010000359        18054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_179005,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000359 DASSGQ010000000
VERSION     DASSGQ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18054
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179005"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..930)
                     /locus_tag="VFI55_15935"
     CDS             complement(70..930)
                     /locus_tag="VFI55_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7075554.1"
                     /translation="MSHSTSLQGKTMFISGASRGIGLEIAKRAAQDGANIALVAKTAE
                     PHPKLPGTVFTAAKELEDAGGQALPIVGDVRDPDSVDSAVAKAVEQFGGIDICVNNAS
                     AINLGSIAEVPMKRFDLMNGIQVRGTYAVSQACIPHMQGRENPHILTLSPPVLLDKKW
                     LKPTAYMMAKYGMTLCALGIAEELREAGIASNTLWPRTLVATAAVQNLLGGDAAMGRA
                     RKPAVYADAAYVILNKPSREYTGNTALCEDVLVESGVTDLSVYDCVPGSTLGVDFWVD
                     AVNPPGYTGP"
     gene            1194..1952
                     /locus_tag="VFI55_15940"
     CDS             1194..1952
                     /locus_tag="VFI55_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7075555.1"
                     /translation="MLVSPVALSVLAGDTAAEGTVLTEMADSEDGGDGVAGPEITKAA
                     ASAATPEETREESDAELTARFERDAIPLLDQLYGGALRMTRNPADAEDLLQETMVKAY
                     AGFRSFRAGTNLKAWLYRILTNTYINSYRKRQRQPAEYPTDEITDWQLASNAEHSSTG
                     LRSAEVEALEGLPDNEIKQALQALPEEFRMAVYYADVEGFPYKEIAEIMDTPIGTVMS
                     RLHRGRRQLRGLLADVAKERGFTRGQQAHEEVSS"
     gene            1949..2251
                     /gene="rsrA"
                     /locus_tag="VFI55_15945"
     CDS             1949..2251
                     /gene="rsrA"
                     /locus_tag="VFI55_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HET7075556.1"
                     /translation="MSDFAGPADLCPNDHSHGDVGCAEVIRQVWLLLDGECKPDTREK
                     LRRHLEACPGCFQHYGLEERIKALIAIKCSGEKAPAGLRERLRLEIQRTTIVRRLP"
     gene            2563..2778
                     /locus_tag="VFI55_15950"
     CDS             2563..2778
                     /locus_tag="VFI55_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HET7075557.1"
                     /translation="MAEDVRAEIVASVLEVVVSEGDQIGKGDIVVLLESMKMEIPVLA
                     EIDGTVSKVSVSVGDVIQAGDLIAVIS"
     gene            2836..4338
                     /locus_tag="VFI55_15955"
     CDS             2836..4338
                     /locus_tag="VFI55_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HET7075558.1"
                     /translation="MSTLGDLLAEHTMLPGNAVDHLHAVVGEWQLLADLSFADYLMWV
                     RRDDGVLVCVAQCRPNTAPTVLQTDSVGSVVAAERLPLVANTFACGVAVRENDAVQEH
                     SWQLPGPHVEASPVRYGGHVVAVLTRHQTAIAADRTSGQLETAYQQCATDLVQMLAEG
                     TFPDVGDVVMSRSTPRAGDGFIRLDVDGVVAYASPNALSAYHRMGLTSDLEGHHLIEV
                     TRPLISDPFEAQEVAEHVRDLLAGGKSMRMEVDAGGATVLLRTLPLVVHGVGAGAAVL
                     IRDITEVKRRDRALISKDATIREIHHRVKNNLQTVAALLRLQARRTVNAEGREALIES
                     VRRVSSIALVHDALSMSVDEQVNLDEVIDRILPIMNDVASVDRPIRINRVGDLGVLDS
                     DRATALIMVITELVQNAIEHAFDPAAHEGSVTIRAERSARWLDVVVHDDGRGLPDGFS
                     LEKSDSLGLQIVRTLVSAELDGTLGMRQAPARGTDVVLRVPIGRRARLLL"
     gene            complement(4434..4688)
                     /gene="whiB1"
                     /locus_tag="VFI55_15960"
     CDS             complement(4434..4688)
                     /gene="whiB1"
                     /locus_tag="VFI55_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator WhiB1"
                     /protein_id="HET7075559.1"
                     /translation="MDWRHKAVCRDEDPELFFPVGNSGPALAQIADAKLVCNRCPVTT
                     ECLGWALNTGQDSGVWGGMSEDERRALKRRNARTKARSGV"
     gene            complement(4960..5937)
                     /locus_tag="VFI55_15965"
     CDS             complement(4960..5937)
                     /locus_tag="VFI55_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET7075560.1"
                     /translation="MRAVLIVNPTATSTTPAGRDLLAHALKSQLQLTVEHTNHRGHAA
                     ELAHAAAADGVDLVVVHGGDGTVSGVVNGLLGRPGAPGPIGPVPAVAVVPGGSANVLA
                     RSLGISKDPVAATNQLIQLLDDYRRHPTWRRIGLIDCGEQWAVLNAGMGVDAEVVAAV
                     EARRDKGFKVTPMRYWRVAVPVAIAFSRREPTLTLELPGRDPVAGVHFVWVSNTTPWT
                     YSDNTPLVTNPGCSFESGLGVFALTSMKVFPTLRLVRQILSKGPKPNVKQLVRDDDAA
                     CLRVTSTEAPIASQFDGDYLGLRQTMTFRSVPDVLPVVAPPRKKALDLR"
     gene            6022..6504
                     /locus_tag="VFI55_15970"
     CDS             6022..6504
                     /locus_tag="VFI55_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075561.1"
                     /translation="MRAAVRREVPGYADAVNPSASPPAPVRGAGVIVAVQGVAALVVA
                     VVLVVRAIAGADQRVVNGLGTAVWFVLVGGVVFAAGRALVVGKRWGRGLAVVTQLLLL
                     PVAWYLAVGSHQPAFGIPVGVVAVTVLVLLFSPAALRWAAGGGQEDSASSPNPGPDSR
                     "
     gene            complement(6456..6938)
                     /locus_tag="VFI55_15975"
     CDS             complement(6456..6938)
                     /locus_tag="VFI55_15975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7075562.1"
                     /translation="MTEIIRRAIPEDAADITDMIHALAEFEHAPDQCTVTEKQISTAL
                     FGDAPTVYGHVAELDGEIAAMGLWFLNFSTWDGVAGIYLEDLFVRPRFRRRGLARALL
                     AALAGVCVDHGYTRLSWAVLDWNRDAIALYDGVGAEPQREWITYRLSGPGLGELAESS
                     "
     gene            complement(6935..8035)
                     /locus_tag="VFI55_15980"
     CDS             complement(6935..8035)
                     /locus_tag="VFI55_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HET7075563.1"
                     /translation="MRPGEPPFALCGPTGTLIADGVRSRYSDVRAAQTALRSGEVPIV
                     LGALPFDVERPAALLVPGTVVSSDGPSDWPTGPLPPVRIAAAVPTPADYRDRISRARD
                     ELAAPGNPLRKVVLARALQLVADAPLDARVILGRLIAADPTAYGYLVDLTAAGDEYVG
                     TALVGASPELLVSRSGDRVVCQPFAGSAPRAADPGLDAANGAALAGSAKNRHEHQLVI
                     DTMRAALEPLCDDLMIAPEPQLTRTAAVWHLCTPISGRLRDKSTTAIDLALALHPTPA
                     VGGVPTKAATELIAELEGDRGFYAGAVGWCDAVGDGHWVVSIRGAQLSADRRTALARA
                     GGGIVAESDPDDEVSETTTKFATILSALEVDR"
     gene            complement(8150..8740)
                     /locus_tag="VFI55_15985"
     CDS             complement(8150..8740)
                     /locus_tag="VFI55_15985"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014712238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HET7075564.1"
                     /translation="MGLHNHRLVLLRHGETEWSKSGQHTGRTDVELTEAGQTQAKLAG
                     RVLGELDLVDPLVISSPRQRTIATAELAGLSIDEVSPLLAEWDYGSYEGLTTQEIQKS
                     VPDWLVWTYGCPGGESVAQVGERADHAVALALASMDARDVVFVSHGHFSRAVITRWVE
                     LPLLEGSRFGMATASIGVCGFEHGVRQLVALGLTGQ"
     gene            8907..9707
                     /locus_tag="VFI55_15990"
     CDS             8907..9707
                     /locus_tag="VFI55_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET7075565.1"
                     /translation="MTKTRVLAVANQKGGVAKTTTVASLGAAMVAAGRRVLLVDLDPQ
                     GCLTFSLGQDPDKLPISVHEVLLGEVEPSAALVTTIEGMTLLPANIDLAGAEAMLLMR
                     AGREYALKRALAKLSDQFDVVVIDCPPSLGVLTLNGLTAADEVIVPLQCETLAHRGVG
                     QFLRTVADVQQITNPDLRLLGALPTLYDSRTTHTRDVLLDVADRYDLPVLAPPIPRTV
                     RFAEATASGSSVMAGRRNKGSAAYSQLAQALLKHWKTGKALPTFVVEV"
     gene            complement(9728..10963)
                     /locus_tag="VFI55_15995"
     CDS             complement(9728..10963)
                     /locus_tag="VFI55_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075566.1"
                     /translation="MVRPERRTKGDIMAAAAIAAIVAVSASLIWWTSDARATISRPAV
                     VPAPAPTPAREVPATLNQLWTAASPATTAPIVVSGTVATGDGRRVDGRDPGTGQSRWS
                     YARDSDLCGVSWVYHYAVAVYHDDRGCGQVSTLDGATGHRGAARSGYADPRVRLSSDG
                     TTVLSAGDTRLELWRSDMVRMLAYGETDARVKPSSRGLHSGCRLISAAASSSAVSVLE
                     SCAQQADLRLVLLRPGKEDDEPQQHVVSEPGIGADSGARVLTVWENNTAVYLPAMSGA
                     QPRVDVIDDTGTTVSSTLLPKAPSSSATVSQAGNVVTWWTGDSLMVFEASNLTLRYTI
                     AAGDDTAPLGPGVMMAGRLLVPVTGAIGVYDPVSGANERYIPVNRAPGTSQPGAAVVP
                     AVSGSRVFEQRGDTLVALG"
     gene            complement(10978..12594)
                     /locus_tag="VFI55_16000"
     CDS             complement(10978..12594)
                     /locus_tag="VFI55_16000"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7075567.1"
                     /translation="MTALTSKTELTPPTTFAELGVRDEIVRALTEKGIESPFAIQELT
                     LPLALAGDDVIGQARTGMGKTLAFGVPMLQRISSGTGVRPLTGAPRALVVVPTRELCL
                     QVTDDLAAAAKYLTAQKGDTAGERPLSVVSIYGGRAYEPQIDALRAGADVVVGTPGRL
                     LDLCQQGHLQLGGLSMLVLDEADEMLDLGFLPDIERILRQIPADRQSMLFSATMPGPI
                     ITLARTFMVQPTHIRAEAPHSAATHDTTEQFVYRAHALDKVELVSRVLQAEGRGATMI
                     FTRTKRTAQKVADELAERGFKVGAVHGDLGQIAREKALKAFRNGDVDVLVATDVAARG
                     IDIDDITHVINYQIPEDEQAYVHRIGRTGRAGKTGIAVTLVDWDELPRWTMIDKALGL
                     ESPDPAETYSNSPHLYAELGIPADAGSTVGVPRKSQARRRDPERNGAGEGPNPEKSGG
                     TRPGARRRRTRGGQAATGHAAGDGVVEARTNGDARTNGDARTNGDAPAETPSGAAAGK
                     SGPSRRRRRRRKPTEGTAATAATAAPAHAN"
     gene            12858..13565
                     /locus_tag="VFI55_16005"
     CDS             12858..13565
                     /locus_tag="VFI55_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HET7075568.1"
                     /translation="MTRPTWKPSSADPVDVSTTSRLTADHPGVNELFAVLAYGEIAAF
                     YRLTDEARMAPNLRGRISMASMAAAEMKHYELLRDSLESRGVDVVSAMSKYVSALENY
                     HRLTMPSTWLEALVKTYVGDALAADLYLEIADGLPDEVADVVRSALAETGHSLFVVAE
                     VRAAVTASSKQRSRLALWSRRLFGEAITQAQYVLAEHDELVDLVVSGPGGLGQLSAFF
                     DRLQDTHDRRMRDLGLS"
     gene            complement(13575..14261)
                     /locus_tag="VFI55_16010"
     CDS             complement(13575..14261)
                     /locus_tag="VFI55_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmpS family transport accessory protein"
                     /protein_id="HET7075569.1"
                     /translation="MVDDPVHDYPDDDSYDGADSDVDDGYDELAWAPNGRWRPVTAIL
                     GAVVALGAVATAVIINSGDSASIKATVGAPPPLTSVPSAPKATAPPSASLSPAPRTSP
                     GPLSPETVTTVPPSMAPTETPTAAPPPIGAPPPVELNPRTVVYSVTGAKQLLDLVTVV
                     YTDARGYPRTEFNVSLPWSKAIVLHPGVQTESVVATSFYGRLNCSIVNAAGQTVLAST
                     GNGIMTTCTL"
     gene            14500..14757
                     /locus_tag="VFI55_16015"
     CDS             14500..14757
                     /locus_tag="VFI55_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HET7075570.1"
                     /translation="MEVKIGITESPRELLLTSTQTPDEVEELVTSALRDESGLLSLTD
                     DRGRRFLVQTSKIAYVEIGVADSRRVGFGIGESAARAARSE"
     gene            complement(14744..15430)
                     /locus_tag="VFI55_16020"
     CDS             complement(14744..15430)
                     /locus_tag="VFI55_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7075571.1"
                     /translation="MSDLAKSAARRAVRPVDRGRPGEGVATANRRGNRLPRDERRGQL
                     LVVASDVFVDHGYHAAGMDEIADRAGVSKPVLYQHFSSKLELYLAVLQRHVENLVSGV
                     QQALSTTTDNRRRLHAAVQAFFDFIEHDSQGYRLIFENDYVTEPEVAAQVRVATESCT
                     DAVFALISQDSGLDPHRARMIAVGLVGISVDCARYWLDSDRPISKADAVDGTVAFAWG
                     GLSHVPLTRS"
     gene            15626..16651
                     /locus_tag="VFI55_16025"
     CDS             15626..16651
                     /locus_tag="VFI55_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3152 domain-containing protein"
                     /protein_id="HET7075572.1"
                     /translation="MTSQRPMRSSGRVPVLRDEWREPLRALRDPIAGDAGRPRVDRDR
                     QRQWRKQTWLGRFVATYGWRAYALPVLVVLTVLVIYQTVTGTSATQPAASQPIQGPPA
                     IGSVGTSIIDAPPRGLAAFDANLPAGTLPDGGPFTEAGDKTWHIVPGTTPQIGQGTAK
                     VFRYTVEVENGIDSTMFGGDNAFAEMVDQTLANPKGWTHNPQFAFIRIDDGKPDFRIS
                     LVSPVTVRDGCGYEFRLETSCYNPVYGPDRQARVFINEARWVRGAMPFEGDVGSYRQY
                     VINHEVGHAIGYVRHEPCEQQGALAPVMMQQTFSTSNDDAAKFDPDVIKADGKTCRFN
                     PWPYPIA"
     gene            16789..17964
                     /gene="moeZ"
                     /locus_tag="VFI55_16030"
     CDS             16789..17964
                     /gene="moeZ"
                     /locus_tag="VFI55_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/sulfurtransferase MoeZ"
                     /protein_id="HET7075573.1"
                     /translation="MPPLVEPAPRLSRDEVARYSRHLIIPGLGIDGQKRLKNARVLVI
                     GAGGLGAPTLLYLAAAGVGTIGIVDFDVVDESNLQRQIIHGVADVGRPKAQSARDSIA
                     AINPLVHVRLHEFRLDRDNAVDLFKQYDLIVDGTDNFATRYLVNDAAVLAEKPYVWGS
                     IYRFEGQVSVFWEDAPNGRGLNYRDLYPEPPPAGMVPSCAEGGVLGIVCASIASVMGT
                     EAIKLITGIGEPLLGRLMIYDALEMSYRTITIRKDPSAAFRPKITELIDYEQFCGVAS
                     DDAARVATGSAITPRELRELLDSGKKLALIDVREPVEWDIVHIDGAQLVPQSSINSGE
                     GLALLPHDRMPVLYCKTGVRSAEALATVKKAGFSDAVHLQGGIVAWAEQMQPDMVMY"
BASE COUNT         2881 a         5855 c         6237 g         3081 t
ORIGIN      
        1 aaatttcttg gatgaaccag gagagcgtcc ctaagccatt cccgtcgcgt caggcggcgg
       61 gagcggtgcc tatgggccgg tgtacccggg cgggttgacg gcatccaccc agaagtcgac
      121 gcccagcgtc gaaccgggca cgcagtcata caccgacaag tcggtgacgc cggattccac
      181 cagcacgtct tcgcacagcg ccgtgttgcc ggtgtactcg cgggacggct tgttgaggat
      241 gacgtaggcc gcgtcggcgt acaccgccgg cttgcgggct cggcccatcg ctgcgtcacc
      301 gccgagcagg ttctgcaccg cggcggtggc caccagcgtg cgcggccaca gcgtattcga
      361 tgcgatgccc gcctcacgca gctcttcggc gatccccagc gcgcacagcg tcatgccgta
      421 cttggccatc atgtaagcgg tcggcttgag ccatttcttg tccagcagga ccggcggtga
      481 cagcgtcagg atgtgcgggt tctcccggcc ctgcatatga ggaatacagg cttgtgagac
      541 cgcgtacgtg ccgcgcacct ggatgccgtt catcaggtcg aaccgcttca tcggcacttc
      601 ggcgatggac cccaggttga tggccgacgc gttgttgacg cagatgtcga tgccgccgaa
      661 ctgctcgacg gccttggcca ccgccgagtc gaccgaatcc gggtcgcgga cgtccccaac
      721 gattggcaaa gcctgtccgc ccgcatcttc gagctccttc gcggcggtga acaccgtacc
      781 cggcagcttc gggtgcggtt cggcggtctt agcgaccagc gcgatgttgg cgccgtcttg
      841 agcggcgcgt ttggcgatct cgaggccgat gccgcgactc gcgccagaga tgaacatggt
      901 cttgccctgg agggacgtgg agtgggacat ggcgtcacat taacgccctg cgccgctcgg
      961 ccggaccggg ggaccgcact ctctgatccc gggtcggcgc ggccccgaaa ccacgcggga
     1021 aatagccggg aaatagcacg gtggcaaacg ccgttgatgg aggtggttct gatgcaggtg
     1081 aggcaggccc ggcagacact gtcgtcggca gcctttagat tgggaggagc agtctggtgt
     1141 cagcggcgat gagcctgttg gaccgtccaa cgggagccga gcggttggct cgcgtgcttg
     1201 tgagtccagt ggcactttcg gtgttggccg gcgacaccgc agcagaaggg accgtgttaa
     1261 ccgaaatggc cgactccgaa gatgggggag acggggtggc ggggccagag attaccaagg
     1321 ccgccgcgtc tgccgcgact cctgaagaga ctcgcgaaga gagtgacgcc gaacttacgg
     1381 cgcgtttcga gcgcgacgcc attccgctgc tggaccagct ctacggtggt gcgctgcgca
     1441 tgacgcgcaa tcccgccgac gcggaggacc tgctgcagga gacgatggtg aaggcctacg
     1501 ccggattccg gtcattccgg gccggcacca atctcaaggc gtggctttac cggattttga
     1561 ccaataccta tatcaacagc tatcgcaaga ggcagcgtca accggcggag tatccaaccg
     1621 acgagatcac cgactggcaa ttggcgtcca acgccgagca ttcgtcgacc ggcctgcgtt
     1681 cggcggaggt ggaagcgctc gaggggctgc ccgacaacga gatcaaacag gcgctgcagg
     1741 cgttgcccga agagttccgg atggcggttt actacgccga cgtcgaaggt ttcccttaca
     1801 aggaaatcgc tgagatcatg gatacgccga tcggcacggt aatgtcgcgg ctgcaccgcg
     1861 gcaggcgtca gctgcgtggc cttttggccg atgtggccaa ggagaggggt ttcacccgcg
     1921 gtcagcaggc gcacgaggag gtgtcgtcat gagcgatttc gccggccccg ccgacctgtg
     1981 ccccaacgac cattcccacg gcgatgtcgg ctgcgccgag gtgatccgtc aggtctggct
     2041 tctgctcgac ggtgagtgca agcccgacac tcgcgagaaa ctgcgccgac acctcgaggc
     2101 atgccccggg tgcttccagc attacggcct agaggagcgg atcaaggcgt tgatcgcgat
     2161 aaagtgcagc ggggagaagg cgcccgcggg cttacgcgaa cgcctcaggc tcgaaattca
     2221 gcggacaacc atcgtgcggc ggttgcccta gcccgatcag gtgttcggcc gcttgccgtg
     2281 gttggccttg ctgtacttac ggtcgcgctt cttgcggcca cgcttggcca tatccctcga
     2341 acctccgaca atcggtcgtc atatcgggcg tctgacaccc gttaggctgt cgcaagtctc
     2401 tcatgggacg cccgagacca cggcctcggc ccggctgcgg cttgttcagg gccggcatgc
     2461 cgcggtcaag gccggccctg tggttcgata tacctgagcc tgatgaaagc ttgatgcacc
     2521 agcagccagc atgcgcaaat ggccgatacg agtggggtga agatggccga agatgttcgc
     2581 gccgagatcg tggccagcgt gctcgaagtc gttgtcagcg agggtgacca gatcggcaag
     2641 ggcgacatcg tggtgctgct ggagtcgatg aagatggaaa tccccgtcct ggcggaaatc
     2701 gacggcaccg tgagcaaggt gagtgtgtcg gtgggcgatg tcattcaggc gggcgacctg
     2761 atcgccgtga tcagctagcc ccaaaaactt ggtatccgtc acaccatccg ccggggaacg
     2821 actcgggagc cattgatgtc cactctcggt gacctgctcg ccgaacacac gatgctgccg
     2881 ggcaatgcgg tggaccacct gcacgcggtg gtcggggagt ggcagctgct ggccgacctg
     2941 tcgttcgccg actacctgat gtgggtgcgc cgcgacgacg gggtgctggt ctgcgtggcg
     3001 cagtgccggc ccaacaccgc gccgacggtt ctgcagaccg attcggtggg cagcgtcgtc
     3061 gccgccgagc ggctgccgct cgtcgccaac accttcgcat gcggtgtcgc ggtgcgggaa
     3121 aacgatgcgg tacaagaaca ttcgtggcaa ctgcccgggc cgcacgtcga ggcttccccg
     3181 gtacggtatg gcggccatgt ggtggcggtg ctgacgcggc accagaccgc gatcgcggcc
     3241 gaccgcacct ccggccaatt ggagaccgca taccagcaat gcgctacgga tcttgtccag
     3301 atgcttgccg agggcacgtt ccccgacgtg ggcgacgtgg tcatgtcgcg gtcgaccccg
     3361 cgggcgggcg acggcttcat ccgcctcgat gtcgacggtg tggtggccta cgccagtccc
     3421 aacgcgctgt ccgcctacca ccggatgggg ctgacgtccg acctggaggg ccaccacctc
     3481 atcgaggtga cacggccgct gatctccgat ccgttcgagg cgcaggaagt cgccgagcat
     3541 gtgcgggact tgctggcggg cgggaagagc atgcggatgg aggtcgacgc ggggggcgcc
     3601 acggtgctgc ttcggacgct gccgctggtg gttcacggcg tcggcgccgg tgctgcggtg
     3661 ctgatccgcg acatcacgga ggtcaagcgt cgcgaccggg ccctgatatc caaggacgcc
     3721 accatccggg agatccacca ccgggtgaag aacaatctgc aaaccgtggc ggcgctgctg
     3781 cgcctgcagg cccggcggac ggtcaacgcc gagggccggg aagcgctgat cgagtcggtg
     3841 cgccgggttt cctcgatcgc gttggtgcac gacgcgctgt cgatgtcggt ggacgagcag
     3901 gtgaacctcg acgaggtcat cgaccggatt ctgccgatca tgaacgacgt ggcgtcggtg
     3961 gacagaccga tccggatcaa ccgtgtcggc gaccttggcg tgctggactc ggaccgcgcg
     4021 acggccctga tcatggtgat caccgagctc gtgcagaacg cgatcgagca cgcattcgat
     4081 ccagcggccc atgaggggtc ggtgacgatt cgtgcggagc gttcggcgcg ctggctcgac
     4141 gtggtcgtgc acgacgacgg gcgcggcctg cctgatgggt tcagcctgga gaaatccgac
     4201 agcctgggcc tgcagatcgt ccggaccctg gtgtcggcgg aactcgacgg cacgctgggc
     4261 atgcggcaag cccccgcccg cggcaccgat gtggtgttac gggtaccgat cggtcggcgg
     4321 gcccgattgc tgctgtaaaa gccctacgga tccgtgcggc ccgacgcctg tcgcggcccc
     4381 acgcaacagt gcggccccga caaattgtcg gggccgcact gtcacgaact gggtcagact
     4441 ccgctgcggg ccttcgtccg ggcattgcga cgcttgagcg cgcgtcgctc atcttcgctc
     4501 atgccgcccc agacgcccga gtcctggccg gtattcagag cccagccgag gcactctgtg
     4561 gtcaccgggc accgattgca gaccagtttc gcgtcagcga tctgcgcgag cgccggcccg
     4621 ctgttcccca cagggaagaa cagctccgga tcctcgtcac gacagaccgc cttgtggcgc
     4681 caatccataa ctagttactc ctcactgagt gcgcagcaag gcgcacggcc ttttttcttc
     4741 ggctgttaac gcgtgcataa gaaatgggcc cgtgccccta catgactttc tgcatgcaac
     4801 ctttcgatcg tttcacaggc tcaacagatg tcaatagcgt gagcaccgcc gtgggctatc
     4861 tcacttcagt gatttcttag ccgagccctc actcagttct actacagtaa ccggctgaat
     4921 ttcacgaaga attttctctg gcgaccgttc catccatcgt taccgcaggt caagcgcttt
     4981 tttgcggggc ggggcaacca ccggaagcac gtcgggaacc gacctgaatg tcatggtttg
     5041 gcgcaacccg aggtaatctc catcgaactg actggcgatc ggtgcttcgg tcgaggtcac
     5101 tcgcaggcaa gcggcgtcgt cgtcgcgaac gagttgtttg acgtttggct tcggcccctt
     5161 cgagaggatc tgccgaacca atctgagggt gggaaacacc ttcatgctgg tgagtgcgaa
     5221 cactccgagg cccgattcga agctgcagcc ggggttcgtc accaacggtg tgttgtcgct
     5281 ataggtccac ggggtggtgt tggacaccca gacgaagtga acgccggcaa cggggtcgcg
     5341 gccgggcaat tccagcgtga gagtgggttc gcgacggctg aaggcgatcg cgacgggcac
     5401 cgccacccgc caatagcgca tgggagtgac tttgaagccc ttgtcgcgtc gcgcttccac
     5461 cgcggcgacg acctccgcat cgacgcccat gcccgcattt aggaccgccc attgttcgcc
     5521 gcagtcgatc agcccgatcc ggcgccacgt cgggtgacgg cggtagtcgt cgagcagctg
     5581 gatgagttga ttggtggcgg cgaccgggtc cttggaaatc cccagcgacc gcgccagcac
     5641 attcgccgac ccgcccggca ccaccgcgac cgcaggcacc gggccgatgg ggcccggagc
     5701 gccggggcgg cccagcaagc cgttgaccac tccgctcacc gtgccgtcac cgccgtgcac
     5761 gacgaccagg tcgaccccgt ccgcggcggc cgcgtgtgcg agctcggcgg cgtggccacg
     5821 gtggttggtg tgctcaacgg tgagctgcag ttggctcttg agtgcgtggg ccagcaggtc
     5881 gcgaccggct ggggtggtgg aagtggctgt tgggttgacg atgagcacgg cccgcatgat
     5941 ggacgagctt aagcggtgcg acgatgcgcg ccgctgtgcg gcgcgaggag gagcatcgca
     6001 gtcgggagca gcggtgcgac gatgcgcgcc gctgtgcggc gcgaggtgcc tggctacgct
     6061 gacgctgtga atccgtcggc gtcgccaccg gcccccgtgc gcggtgcggg cgtcatcgtg
     6121 gcggtacagg gtgtggcagc gctggtggtg gcggtggtgc tggtggtgcg ggcgatcgcg
     6181 ggagccgacc agcgtgtggt caacggcctg ggcacggcgg tctggttcgt tctcgtcggc
     6241 ggcgtggtgt tcgcggccgg acgcgcgctg gtcgtcggta agcgttgggg ccgcgggctg
     6301 gcggtagtca cccagttgct cctgctgcct gtcgcgtggt acctcgcggt gggctcgcat
     6361 cagccggcct tcgggattcc cgtgggcgtc gtggcggtga cggtgctggt cttgctcttc
     6421 agtccggcgg ccctgcgttg ggccgccggc ggcggtcagg aggattcggc cagctcaccc
     6481 aaccccgggc cggacagccg ataggtgatc cactcgcgct gcggctccgc gccgaccccg
     6541 tcgtacagcg cgatggcatc gcggttccag tccagcaccg cccacgacaa ccgcgtgtag
     6601 ccatggtcga cgcacacacc ggccagtgcg gccagcagcg cacgggccag gccccggcgg
     6661 cggaacctcg gcctgacgaa caggtcttcg aggtagatgc ccgcgacacc gtcccacgtg
     6721 gagaagttga ggaaccacag acccatggcg gcaatctcgc cgtcaagttc ggcgacatgg
     6781 ccgtacaccg tgggggcgtc accgaaaagc gcagtggaaa tttgtttttc ggtgacggtg
     6841 cattgatcgg gggcgtgttc gaattcggcc agcgcgtgga tcatgtcggt aatgtcggcg
     6901 gcgtcttccg gtatggcgcg gcgaatgatt tctgtcatcg gtcaacttcc agcgcgctga
     6961 gtatcgtggc gaatttcgtt gtggtttctg atacttcgtc gtcggggtcg gattcagcga
     7021 cgatgccgcc gccggcgcgg gccagcgccg tgcggcggtc tgccgagagc tgcgcaccac
     7081 ggatcgacac cacccagtga ccgtcgccga cggcgtcgca ccagcccacg gctccggcat
     7141 aaaagccgcg gtcgccctcc aactcggcga tgagctcggt cgcggccttc gtcggcaccc
     7201 cgccgaccgc gggggtgggg tgtaacgcca acgccagatc gattgcggtg gttgacttat
     7261 cgcggagccg gccgctgatc ggcgtacaca gatgccagac cgccgcggtg cgcgtcagct
     7321 ggggctccgg cgcgatcatc aggtcgtcac acaatggctc cagggcggcg cgcatggtat
     7381 cgatcaccag ctgatgctcg tggcggttct tggccgatcc ggccagtgcg gcgccattgg
     7441 cggcgtcgag tcccgggtcg gcggcgcgtg gggccgaacc ggcgaacggc tggcacacga
     7501 cacggtcgcc ggaccgcgac accaggagtt ccgggctggc gcccaccagc gccgtgccaa
     7561 cgtactcgtc gccggcggcg gtcaggtcga cgagatagcc gtaggcggtc gggtcggccg
     7621 cgataaggcg gcccaggatg acgcgggcat ccagcggagc gtcggcgacc agttgcaacg
     7681 cgcgggccag caccaccttg cgcaacgggt tccccggtgc ggcgagttcg tcacgtgcgc
     7741 ggctgatccg gtcgcggtag tcggccggcg tcggcacggc ggcggctatg cgcacgggag
     7801 gtagcgggcc cgtcggccag tccgacggtc cgtcggagct gacgaccgtg cccggcacca
     7861 gcagggccgc cggcctttcc acatcgaaag gtaaggcgcc caacactatt gggacttccc
     7921 ccgaccgcag cgccgtttgg gccgcgcgca cgtcgctgta gcggctccgc accccgtcgg
     7981 ctatcagggt ccccgtcgga ccgcacagcg cgaatggcgg ctcgcccggt ctcaattgcc
     8041 cggctccttt tcgcgccgct ggacggcgct catcgtcgcc gggcccggtc tcaattgccc
     8101 ggctcctttt cgcgccgctg gacggcgcgc atcgtcgccg ggccgacact cactgaccgg
     8161 tcaatccgag cgctacgagt tgtcgcaccc cgtgctcgaa cccgcacacg ccgatcgagg
     8221 ccgtggccat gccgaagcgg ctgccctcga gcaacggcaa ctcgacccag cgggtgatca
     8281 cggcgcggga gaagtggcca tggctcacga acaccacgtc gcgcgcgtcc atgctcgcca
     8341 gcgccagcgc gaccgcgtgg tcggcgcgct caccgacctg cgccacgctc tcaccaccgg
     8401 ggcatccgta cgtccacacc agccaatcgg gcaccgattt ctggatctcc tgggtggtca
     8461 ggccctcgta ggacccgtag tcccactcgg cgagtagcgg agatacctcg tcgatgctga
     8521 gtccggcgag ctcggcggtc gcaattgtgc gctgacgtgg gctactgatc accagcgggt
     8581 cgacaaggtc gagttcaccc agcacccgcc cggccagctt ggcctgcgtc tgcccggctt
     8641 cggtcaactc gacgtcggta cggccggtgt gctggcccga tttcgaccac tcggtctcgc
     8701 cgtggcgcag cagcaccagc cggtgattgt gcaagcccat gccgacagat tctgccggac
     8761 gacataccgt ggcgtcggcc gaggcgcttg atggccgaac atgccaggat gggcactgtg
     8821 atcgcgcggc gacgatgcag aggagaccga gtgataacga gtgatggggc gggagcggct
     8881 cccgtgtgcg ggggagagcg gcgcccgtga ctaagacccg agtgctggcg gtggccaacc
     8941 agaagggcgg cgtagccaag acgacgacgg ttgcctcgct gggtgcggcg atggtggctg
     9001 cgggccggcg cgtcttgctg gtcgatctgg atccccaggg ctgtttgacc ttctcgctcg
     9061 gccaggatcc cgacaaacta ccgatatccg tgcacgaggt cctgctgggt gaggtcgagc
     9121 cgagcgccgc actggtcacg accatcgaag gcatgacgct gctgccggcc aacattgacc
     9181 tggcgggcgc cgaggcgatg ctgctgatgc gggccggccg cgaatacgcg ctcaaacgcg
     9241 cgctggccaa actttccgac cagttcgacg tggtggtcat cgattgcccc ccgtcgttgg
     9301 gtgtactcac cctcaacggg ctgacggccg ccgacgaggt gatcgtcccg ctgcagtgtg
     9361 agacgctcgc gcatcgcggc gtcggacagt tcctgcgcac ggtcgccgac gtccagcaga
     9421 tcaccaaccc ggatctgcgg ctgctgggcg cgctgccgac gctgtacgac tcgcgcacca
     9481 cgcacacccg cgacgtgctg ctcgatgtcg ccgaccgcta cgacctgccg gtgctcgccc
     9541 cgcccatccc ccgaacggtg cgcttcgccg aggccaccgc ctcgggctcg tcggtgatgg
     9601 ccggacgcag gaacaaaggt tcggcggcct acagccagct ggcgcaggcg ctgctcaagc
     9661 attggaagac cggaaaagca ctgccgacgt tcgtcgtcga ggtttaacgg gatcgctcga
     9721 cggggcctca gcccagggcc accagggtgt ccccgcgctg ctcgaagacc cgggatcccg
     9781 acacggccgg caccaccgcc gcgccgggct gcgaggtgcc gggcgcccga ttcaccggta
     9841 tgtagcgttc attcgcgccg ctgaccgggt cgtagacgcc gatcgcgccg gtgaccggca
     9901 cgagcagccg tcctgccatc atcacgcccg gtcccaacgg cgccgtgtcg tcgcccgcgg
     9961 cgatcgtgta gcgcaaagtc agattgctcg cctcgaaaac catcagagaa tcgccggtcc
    10021 accaggtcac cacgttcccc gcttgcgaca ccgtcgccga actcgatggc gccttgggca
    10081 gcagcgtgct cgacacggtg gtgccggtgt cgtcgatcac gtcgaccctg ggctgcgcgc
    10141 cggacattgc gggcagatag accgccgtgt tgttctccca gacggtcagc acgcgcgcgc
    10201 ccgaatcggc cccgatcccg ggttcggaca ccacgtgttg ttggggttcg tcgtcctcct
    10261 tgccgggacg caacaggaca agccgcaggt cggcctgctg cgcgcagctc tccagcaccg
    10321 ataccgccga cgagctggcc gccgccgaaa tcagccggca tccggagtgc agtccccgcg
    10381 acgacggttt cacccgggcg tcggtctcgc cgtaggccag catccgcacc atgtccgagc
    10441 gccacagttc cagccgggtg tcgccggccg acaagaccgt ggtgccgtca gaagataagc
    10501 gcacccgcgg atcggcgtag ccgctgcggg cggccccgcg atggcctgtg gcgccgtcga
    10561 gggtgctgac ctgcccgcat ccgcggtcgt cgtggtagac ggcgacggcg tagtggtaga
    10621 cccaggacac cccgcacagg tcgctgtcac gggcgtagct ccagcgggac tggccggtgc
    10681 ccgggtcgcg cccgtcgacc cggcggccgt cgccggtggc gaccgtccca ctcaccacga
    10741 tgggggcggt ggtggctggg ctggcggcgg tccacaactg attcaaggtg gccggcactt
    10801 cgcgggccgg tgtcggggcg ggcgccggaa cgacggcggg ccggctgatg gtggcccggg
    10861 cgtcgctggt ccaccagatc agggacgcgg acacggcgac gattgccgcg atcgccgcgg
    10921 cggccattat gtcgcctttg gtccggcgtt cgggtctgac catgggccag cgcgggctca
    10981 gttggcgtgt gcgggggctg cggtggccgc ggtggccgcc gtgccctcag tgggcttgcg
    11041 gcgccggcgg cggcgccggc tgggcccaga cttgcccgcg gcggcgccgg acggcgtctc
    11101 ggcgggggcg tcgccattgg ttcgggcgtc gccgttggtt cgggcgtcgc cgttggttcg
    11161 ggcctcgacg actccgtcgc cggcggcatg cccggtggcg gcttggccgc cacgggtgcg
    11221 ccggcgccgg gcgccgggac gcgtaccgcc cgacttctcc gggttagggc cttcaccggc
    11281 gccgttgcgc tcggggtcgc ggcgtcgggc ctgcgacttg cggggaacac caacggtgct
    11341 gcccgcgtcg gcggggatgc ccaactcggc atacaggtgc ggcgagttcg aataggtctc
    11401 ggccggatcc ggggactcca ggcccagcgc cttgtcgatc atcgtccagc ggggcagctc
    11461 gtcccagtcg accagggtga cggcgatacc ggtcttgccg gcgcgaccgg tacggccgat
    11521 ccggtgcacg taggcctgtt cgtcttcggg gatctggtag ttgatgacgt gggtgatgtc
    11581 atcgatgtcg atgccgcggg cggcgacgtc ggtggccacc agcacgtcga cgtccccgtt
    11641 tcggaacgcc ttgagtgctt tctcgcgggc gatctgcccg agatcgccgt gcacggcgcc
    11701 cactttgaag ccccgctcgg ccagctcgtc ggcgaccttc tgggcggtgc gcttggtgcg
    11761 ggtgaagatc atcgtcgcgc cgcggccctc ggcctgcagc acccggctga ccagctcgac
    11821 cttgtccagc gcgtgagcgc gatagacgaa ctgctcggtg gtgtcgtgcg ttgccgccga
    11881 gtgcggcgcc tcggcgcgga tgtgcgtggg ctggaccatg aaggtgcggg ccagcgtgat
    11941 gatcgggccg ggcatggtcg ccgagaacag catcgactgc cggtctgcgg gaatttggcg
    12001 caggatgcgc tcgatgtcgg gcaaaaagcc caggtcaagc atctcgtcgg cctcgtcgag
    12061 caccagcatc gacagcccgc ccagctgtag gtgcccctgc tggcacaggt ccagcagccg
    12121 acccggggtg cccaccacga cgtcggcgcc ggcgcgcagc gcgtcgatct gcggctcgta
    12181 ggcgcgtccg ccgtagatgg acaccaccga tagtggacgt tctccggcgg tgtcgccctt
    12241 ttgggcggtc aggtatttgg cggcggcggc caggtcgtcg gtgacctgca ggcacagctc
    12301 ccgggtgggc accacgacca gtgcccgcgg ggcgccggtc agcggccgga caccggtgcc
    12361 gctgctgatg cgttgcagca tcgggacgcc gaaggcgagg gttttaccca ttccggtgcg
    12421 ggcttggccg atgacgtcgt cgccggccag cgccagcggc agggtcagtt cttgaatagc
    12481 aaaggggctt tcgatgccct tttcagtgag tgcgcggaca atttcgtcgc gaacgccaag
    12541 ttcggcaaat gttgttgggg gggtcagttc agttttgctc gtgagtgcgg tcatacgtgg
    12601 atggcaagcc cttcggtgac ggtatccgtg ttgtggtcgg tttctctcgc ggtcacgcgc
    12661 gcacgagttc cgactccgga tacgtcgccg agtcgcggcc cctgctcgat ctgagcgacg
    12721 ctcaggccag gcgggtcagc agtgcacgca catttcgccg gtactgacgg caggaaaaca
    12781 gccaatacct acatcaatca taaccggtcg acagccggca cccctttttg gcttggtccg
    12841 cctactagag tgttgccatg acccggccca cttggaagcc ctcctccgcc gatccagtgg
    12901 acgtctcgac cacctcacgg ctgacggctg atcacccggg cgtcaacgaa ttgttcgcgg
    12961 tgctggcata tggcgagatc gcggcctttt accggctcac cgacgaggcg cggatggcgc
    13021 ccaatctacg gggacggatc tcgatggcga gcatggcggc cgccgagatg aagcactacg
    13081 agctgttgcg cgactcgctg gaaagccgtg gtgtggacgt ggtttcggcg atgtcgaagt
    13141 acgtttcggc gctggaaaac taccaccggc tgaccatgcc gagtacgtgg ctggaagcgt
    13201 tggtgaagac ttacgtcggc gatgccctgg cggcggatct atatctggag atcgccgatg
    13261 ggctgcccga cgaggtcgcc gacgtggtgc gctccgcgct ggcggagacc ggacactcgc
    13321 tgttcgtcgt cgccgaggtg cgcgccgccg tcaccgccag tagtaagcag cgcagccggc
    13381 tggcgctgtg gtctcgtcgc ctgttcggcg aagcgatcac tcaggcccaa tacgtgctgg
    13441 ccgagcacga tgagctggtc gacctggtgg tgtcgggtcc cggcggcctg ggccagctca
    13501 gcgccttctt cgaccgcctg caggacacgc acgaccgacg catgcgcgac ctcggcctga
    13561 gctgacctcg ccgctcagag cgtgcaggtc gtcatgattc cgttgccggt cgacgccagc
    13621 acggtctgac cggcggcatt gacgatcgag caattgagcc ggccgtagaa gctggtcgcg
    13681 acgaccgact cggtctggac gccgggatgc aggacgatcg ccttcgacca cggcagcgac
    13741 acgttgaact cggtccgcgg gtagccgcgg gcgtcggtgt acaccacggt caccaggtcc
    13801 aggagctgtt tcgcgccggt cacgctgtag acgacggtgc gcggattcag ctcgaccggt
    13861 ggcggtgcgc ctatcggggg cggcgcggcg gtgggcgttt cggtcggcgc catgctgggc
    13921 ggcacggtgg tgaccgtctc gggcgacaac ggcccggggg aggtgcgggg cgcggggctg
    13981 agcgacgcgc tgggcggggc ggtggccttg ggtgctgacg gcacggaggt caggggcggc
    14041 ggcgcgccca cggtggcctt gatcgacgcg ctgtccccgc tgttgatgat gacggcggtc
    14101 gcgacggctc ccagggccac cacagcgccg agaatcgccg tgaccgggcg ccaccggcca
    14161 ttgggcgccc acgccagctc gtcgtagccg tcatcgacgt cggagtcggc tccgtcgtag
    14221 ctgtcgtcgt cggggtagtc gtgcaccggg tcatcgacca ggccgacgcg atcggtaaac
    14281 gtgtttctga tggtgccgat gctactgatg tgtcatctgg tgatggggtg acgtgcggcg
    14341 tgttggccca gctgggagct aatcgttatc ttcggccatc ccgtttcgct taccgcgaac
    14401 gtcggggtcc gcggggcatc tgcggcagag cgcgctcgcc gcgtccacta gcctgctgct
    14461 gacacgtctg cgactgcgaa accaacggaa ggggccaccg tggaggtcaa gatcggtatc
    14521 acggagagtc cgcgcgagct gctcctcacc agtacgcaga cacccgatga agtcgaagaa
    14581 ctcgtgacca gcgcactgcg cgacgaatcg ggcctgctca gcttgaccga cgacaggggc
    14641 cgtcgcttcc tggtccagac ctccaagatc gcctatgtcg agatcggtgt cgccgactcc
    14701 cgccgggtcg gcttcggaat cggggaatcg gccgcgaggg ccgctaggag cgagtaagcg
    14761 gcacgtgtga caacccgccc caggcgaacg caacggtgcc gtcgacggcg tccgccttgg
    14821 aaatcggacg gtcagaatcc agccagtacc gggcgcagtc gacgctgatc ccgaccagtc
    14881 ccaccgcgat cattcgggca cggtgcgggt caagtcccga atcctggctg atcagggcga
    14941 acacggcgtc ggtgcacgat tcggtggcga cgcgcacctg ggcggcgacc tcgggttcgg
    15001 tgacatagtc gttctcgaaa atcagccggt acccctggct gtcgtgctcg atgaagtcga
    15061 agaacgcctg gacggccgcg tgtaaccgcc gccggttatc ggtggtagtg ctcaatgcct
    15121 gctgcacacc ggagaccagg ttttcgacgt gccgttgcag caccgccaga tacagctcga
    15181 gcttgctcga aaagtgttgg taaagaacgg gtttgctgac gcccgcccgg tcggcgatct
    15241 cgtccatgcc ggcggcgtgg tagccgtggt cgacaaagac atcgctggcg acgaccagca
    15301 attggccacg acgctcgtcg cgaggcaagc ggttgccgcg gcggttggcg gtcgccacgc
    15361 cctctccggg ccgaccgcgg tcaaccggtc tgacggcacg ccgtgccgct gacttggcga
    15421 gatcgctcat caggtcctca tctgattgtg cgcaaccggc catcggactc caggccgctc
    15481 gcgcgcagct cgtcggatcg acattactac ccgaaacgcc gtggtgatca tcatcgtggc
    15541 cgtcgccgct ggcacaaacc ccgtcggccg ccgcattcgg gcgcgccaag ggcggccacc
    15601 gggtgggctg tgcaatcctg gggaaatgac gtcccagcgg cccatgcgca gcagcggccg
    15661 agtcccggtg ctgcgggacg agtggcgcga accgcttcgg gccctgcgcg acccgatcgc
    15721 cggggatgcc ggacggcccc gggtcgaccg cgaccggcag cgccagtggc gcaaacaaac
    15781 gtggttggga cggttcgtag ccacttatgg gtggcgcgcg tacgcgttgc cggttctggt
    15841 cgtgctcacc gtgttggtca tctaccaaac cgtgaccgga accagcgcaa ctcaaccggc
    15901 ggcgagtcaa cctatccagg gcccgccggc gatcggctcg gtgggcacgt cgatcatcga
    15961 cgcaccacct cgcggcctgg ccgccttcga cgccaacctg ccggccggga cgctgccgga
    16021 cggcgggcca ttcaccgagg cgggcgacaa gacgtggcat atcgtgccgg gcacgacgcc
    16081 acagatcggc cagggcaccg ccaaggtgtt ccggtacacc gtcgaggtcg agaacggcat
    16141 cgactccacg atgttcggcg gcgacaacgc cttcgccgag atggtcgacc agacgctggc
    16201 caatcccaag ggctggacgc acaacccgca attcgcgttc atccggatcg acgacggcaa
    16261 acccgacttc cggatctcgc tggtgtcccc ggtgacggtg cgcgacggct gcggttacga
    16321 attccggctg gagacgtcct gctacaaccc ggtgtacggg cccgaccggc aggcgcgcgt
    16381 cttcatcaac gaggcgcgct gggtgcgcgg agccatgccc tttgaaggcg acgtaggttc
    16441 ctaccggcag tacgtgatca accatgaggt cggccacgcc atcgggtacg tgcgtcacga
    16501 accgtgcgag cagcagggtg ctctggctcc ggtgatgatg cagcagacat tctccacgtc
    16561 caacgacgac gccgccaagt tcgaccctga cgtgatcaag gccgatggga agacctgccg
    16621 attcaacccc tggccgtacc cgatcgccta gcggggcaat cggatctagc gggtacctcg
    16681 ttgccgtcta tccgcacaac tgctgtgatg gttgcagacg acacgtgcag aagttgcgtc
    16741 gcgcaagcta gccgaggaga tgtttcggtg tcgacgtcgc aggcatcact gccgccgctg
    16801 gtcgaacccg caccacggct gagtcgcgac gaggtagccc gctacagccg ccacctgatc
    16861 attcccggcc tgggtatcga cggtcagaag cggctcaaaa acgcccgggt gctggtgatc
    16921 ggcgccggcg gactcggcgc acctaccttg ctctacctgg cagcggccgg agtcggcacc
    16981 atcggcatcg tcgatttcga cgtcgtcgac gagtcgaacc tgcagcgcca gatcatccac
    17041 ggtgtggccg acgtcggacg gcccaaggcc cagtcggcgc gcgactcgat cgccgcgatc
    17101 aacccactgg tccacgtgcg gctgcacgag ttccggctgg atcgagacaa cgccgtcgac
    17161 ctgttcaagc agtacgacct gatcgtggac ggtaccgaca acttcgctac ccggtatctg
    17221 gtcaacgatg ccgcggtgct ggctgaaaag ccgtatgtgt gggggtcgat ctaccgattc
    17281 gagggccagg tctcggtgtt ctgggaggac gcgcccaacg gtcggggcct caactaccgc
    17341 gatctctacc cggagccgcc gcccgccggc atggtgccct cgtgcgccga aggcggcgtc
    17401 ctgggcattg tctgcgcctc gatcgcgtcg gtgatgggca ccgaggcgat caaactgatc
    17461 accggcatag gtgaaccgtt gctcggccgg ttgatgatct acgacgcgct cgagatgagc
    17521 tatcgcacca tcacgatccg caaggacccg tcagcggcat tcaggccgaa gatcaccgaa
    17581 ctcatcgact acgagcaatt ctgtggcgtg gcgtccgacg atgctgcccg ggtggccacc
    17641 ggctccgcca tcaccccgcg ggagttacgt gagctgctgg attccggcaa gaagctggcc
    17701 ctgatcgacg tccgtgagcc cgtcgagtgg gacatcgtgc acatcgacgg cgcacagctg
    17761 gtcccgcagt cgtcgatcaa ctcgggtgag ggtctggcgc tgctaccgca cgaccgcatg
    17821 ccggtgctct attgcaagac gggtgtgcgc tcggccgagg cgctggccac cgtgaagaag
    17881 gccggattct ccgatgcggt ccacctgcag ggcgggatcg ttgcctgggc cgaacagatg
    17941 cagcccgaca tggtgatgta ctgaccgatg ccggtggtct tgtctggtgg cgacccgccg
    18001 cgcccggctt cgccgcgctt gcggtcgccc ctgtgttgtt tggtggcgac ccgc
//
LOCUS       DASSGQ010000360         6929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_330995,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000360 DASSGQ010000000
VERSION     DASSGQ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330995"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VFI55_16035"
     CDS             <1..626
                     /locus_tag="VFI55_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HET7075574.1"
                     /translation="LPPSVLAREVDDDEAIRELTLRAATALGLGTEADIRDYFRLSAQ
                     QVKPALAALLAAGEIERVEVDGWSAPAYLCAGRTVPRRDRGTALLCPFDPLIFFRPRV
                     ERLFGFHYRIEIYTPAAKRQYGYYVWPLLMDGRLVARVDLKADRAANSLRVVGAFGEP
                     DVPRAQVAAALAGELESMASWLGLGGFSVSARGDLAPELRAVTKRAG"
     gene            626..2044
                     /locus_tag="VFI55_16040"
     CDS             626..2044
                     /locus_tag="VFI55_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HET7075575.1"
                     /translation="MAPSTTKELKATLWKAAEKLRGSLSAGQYKDVVLGLVFLKYVAE
                     PHWKPLVDNATAPNIGRLVDEAMTAVMTANPALAGTLPQLYENLDQRRLGELMLVLDS
                     ARFSRQGRHRARDLMGEVYEYFLGSFARAEGRRGGEFFTPPSVVRVIVEVLEPSGGRV
                     YDPCCGSGGMFVQTEHFIDEHDGDPNCVSIYGQESVEQTWRMAKMNLAVHGIDATGLG
                     PACGDTFVNDQHAGVQMDYVMANPPFNIKDWARDERDPRWCFGVPPASNANYAWIQHI
                     LSKLAPGGRAGVVMANGSMSSNSGGEGDIRARIVEADLVSCVVALPGQLFRSTGIPVC
                     LWFFATDKTAGIDRSGQLLFIDARRLGYLVDRAERALTDEEIVRIGDTFRAWSGSASA
                     TAKGLTYQDLPGFCKSASLDDVRAADYALTPGRYVGAPAVEDDGEPIDEKVARLTKDL
                     LSALDESARLEEVVRDQLERLR"
     gene            2041..3096
                     /locus_tag="VFI55_16045"
     CDS             2041..3096
                     /locus_tag="VFI55_16045"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020724791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET7075576.1"
                     /translation="MKVKLGDHLDFSNGSSVPRRASDGRFRVYGANGAIGYAARHNAS
                     GPLIVVGRVGTYCGSLRYCDSDVWVTDNALVCRAKDPQETRYWYYALHTCGLNEHRAG
                     SGQSLLNQRILRDVSVCTVAAHERRRIAESLGALDDKIAANERVIAAAESLMIAIVES
                     VSECAPLSTLAGRSTTLLRPREFDDVVAHFSLPAFDDGAKPRLVAAGAVKSGKFRLTG
                     PCVLVAKLNPRIPRIWDVASLPPQMALASTEFVVLRPIGLDTSALWSALRQPDVVETL
                     QQWVAGTSGSHQRVQPRDLLQLRVRDVRRLGAAQSRTIAGLGALCHARRAECVRLSAL
                     RDALLPLLISGRVRVAR"
     gene            3291..4043
                     /gene="thyX"
                     /locus_tag="VFI55_16050"
     CDS             3291..4043
                     /gene="thyX"
                     /locus_tag="VFI55_16050"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HET7075577.1"
                     /translation="MAETAPLRVQLIAKTEFMAPPEVPWTTDADGGAALVEFAGRACY
                     QSWSKPNPKTATNAGYIKHIIDVGHFSVLEHASVSFYITGISRSCTHELIRHRHFSYS
                     QLSQRYVPEGKSQVVVPPGMEDDPELQQILTAAADASRASYTELLAKLEAKFADQPNA
                     VLRRKQARQAARAVLPNATETRIVVTGNYRAWRHFIAMRASEHADVEIRRLAIECLRQ
                     LAGVAPTVFADFEVSTLADGTEVATSPLATEA"
     gene            4149..5051
                     /gene="dapA"
                     /locus_tag="VFI55_16055"
     CDS             4149..5051
                     /gene="dapA"
                     /locus_tag="VFI55_16055"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET7075578.1"
                     /translation="MTTVGFDAPARLGTLLTAMVTPFGADGSLDTTAAGRLAKHLVDG
                     GCDGLVVSGTTGESPTTSDDEKRELVRVVLEAVGDRARVIAGAGTYDTAHSIRLAKAA
                     AAEGAHGLLLVTPYYSKPPQSGLRAHFTAVADATELPVLLYDIPGRSGTAIEPDTIRA
                     LASHPNIVGVKEAKADLHNSAQIIAETGLAYYSGDDALNLPWLAMGATGFISVIAHLA
                     SGQLRELLSAFNSGDIGTARKIHVAVAPLCNAMARLGGVTMSKAGLRLQGIDVGDPRL
                     PQMPATREQIDALAADMRAASVLR"
     gene            5071..6747
                     /locus_tag="VFI55_16060"
     CDS             5071..6747
                     /locus_tag="VFI55_16060"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET7075579.1"
                     /translation="MNEDLAPPGPLAPGGLRVTALGGISEIGRNMTVFEHLGRLLIID
                     CGVMFPTHDEPGVDLILPDLRHIEDRLDDVEALVLTHAHEDHIGAIPFLLRLRPDIPV
                     VGSRFTLALVAAKCREYRIKPVFVQVAEGQHSTHGVFQCEYFAVNHSIPDALAIAVHT
                     GAGTVLHTGDIKLDQLPPDGRPTDLPGMSRLGNAGVDLFLCDSTNSEIPGVGPSESEV
                     GPSLHRLIRGADGRVIVACFASNVDRVQQIIDAAVALGRRVSFVGRSMVRNMGIAREL
                     GFLRVDDSDLIDISAAEMMAPDQVVLITTGTQGEPMAALSRMSRGEHRSITLTAEDLV
                     ILSSSLIPGNEEAVYGIIDSLAKVGARIVTNQQVRVHVSGHAYAGELLFLYNGVRPRN
                     VMPVHGTWRMLRANAKLAARAGVPEESILLAENGVSVDLVAGKAAIAGAVPVGKMFVD
                     GLITGDVGDITLGERLILSSGFVAVTVVVKRGTGLPAVVPQLLSRGFSEDPKALEPAV
                     RKVEAELEQLAAAGVTDPVRIAQGVRRIVGKWVGETYRRQPMIVPTVLEV"
     gene            complement(6843..>6929)
                     /locus_tag="VFI55_16065"
     CDS             complement(6843..>6929)
                     /locus_tag="VFI55_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075580.1"
                     /translation="ELMRRYVAPTGRKLKGSQLEWSAYAEKI"
BASE COUNT         1110 a         2302 c         2367 g         1150 t
ORIGIN      
        1 tgctgccgcc gagcgtgctg gctcgcgagg tcgacgatga cgaggcgatc cgcgagctca
       61 cgctgcgcgc cgccaccgcg ctgggcctgg gcaccgaggc cgacatccgc gattacttcc
      121 ggctgtcggc ccagcaggtc aaacccgctc ttgccgcgct gctggccgcc ggtgagatcg
      181 agcgggtcga ggtcgacggc tggtccgcgc ccgcgtatct gtgcgccggc cggacggtgc
      241 cgcgccgcga ccgcggcacc gcgctgctgt gcccgttcga cccgctgatc ttcttccggc
      301 cccgagtcga gcgactgttc ggatttcact accgcatcga gatctacacc ccggcggcca
      361 agcgccaata cggctactac gtgtggccgc tgctgatgga cggccggctg gtcgcgcggg
      421 ttgacctcaa ggccgaccgg gccgccaact cgctgcgcgt cgtcggcgca ttcggcgagc
      481 cggacgtgcc ccgagcgcag gttgccgcgg cgctggccgg tgagctcgag tcgatggcgt
      541 cctggctggg cctgggcggc ttcagcgtgt cggctcgggg tgatttggcg ccagagctac
      601 gcgcggtcac caagcgggcc ggctgatggc cccgtcgaca acgaaagagc tcaaggccac
      661 gctgtggaag gccgccgaaa agctgcgcgg atccctctcg gccggacagt acaaggacgt
      721 ggtcctcggg ctggtgttcc tgaagtatgt cgccgagccg cattggaaac ccttggtgga
      781 caatgcaaca gctcccaaca tcggtcggct cgtcgacgag gccatgactg ccgtgatgac
      841 ggcgaatccc gcgctggccg gaacgctgcc gcagctctac gaaaacctgg atcagcgccg
      901 gctcggggaa ctgatgctcg tgctcgacag tgcgcgattc agccggcagg gcaggcaccg
      961 cgcgcgagac ctgatggggg aggtgtacga gtacttcctg ggcagcttcg cccgtgcgga
     1021 gggcaggcgg ggcggcgaat tcttcacccc gcccagcgtt gttcgggtga tcgtcgaggt
     1081 cctcgaaccg tcgggcggac gggtgtacga cccgtgctgc ggatcgggcg gaatgttcgt
     1141 gcagacagag catttcatcg acgagcacga cggcgatccc aattgcgtct cgatctacgg
     1201 ccaggagagt gtcgagcaga cctggcggat ggccaagatg aacctcgccg tccacggcat
     1261 cgacgccaca ggcctgggcc cggcctgtgg cgacacgttc gtcaacgatc aacacgcggg
     1321 cgtccaaatg gactacgtga tggccaatcc gccgttcaac atcaaggact gggcccgcga
     1381 cgaaagagac ccgcgctggt gcttcggtgt tccgccggcc agcaatgcga actacgcctg
     1441 gatccagcac attctgtcca agctggcacc gggcgggcgg gccggggtgg tgatggccaa
     1501 cggctcgatg tcatcgaact cgggcgggga aggcgacatt cgcgcgcgga tcgtcgaggc
     1561 ggacctggtg tcctgcgtgg tcgcgctgcc cgggcagctg tttcgcagca cgggaattcc
     1621 ggtgtgtctg tggttcttcg cgacggacaa gacggctggc atcgaccggt cgggtcagct
     1681 gctgttcatc gatgcccgca ggctcggcta cctggtggat cgcgccgagc gcgcactgac
     1741 cgacgaggag atcgtccgga tcggcgacac cttccgcgca tggtccggat cggcgtcggc
     1801 caccgccaaa ggccttacct accaagacct tccgggattc tgcaaatccg cgtcgctgga
     1861 cgatgtcagg gccgcggact acgcgctcac gcccggccgg tatgtcggtg cgcctgccgt
     1921 cgaggacgac ggggagccga tcgatgagaa ggtcgcgcgg ctgaccaaag acttgctgtc
     1981 ggcgctcgac gagtccgcgc gactcgaaga ggttgtgcgc gaccaactgg agcgcctgcg
     2041 gtgaaggtca agctgggtga ccatctcgac ttcagcaacg gaagcagcgt gccgaggcga
     2101 gcgtcggacg ggcgctttcg cgtctacggc gccaacgggg ctatcggtta cgcggcacgc
     2161 cacaacgcga gcggtccgct gatcgtcgtc gggcgcgtcg gaacgtactg cggcagcctg
     2221 cggtattgcg attcggacgt ctgggtcacc gacaacgcgc tggtgtgccg ggccaaggac
     2281 ccgcaagaga cgcgatattg gtattacgcg ctgcatacct gcgggctcaa cgaacaccga
     2341 gcgggatcgg gacagtccct gctcaaccag agaattcttc gtgacgtgtc ggtttgcacc
     2401 gtcgcagcac acgaacgccg ccggatcgcc gagtcgctcg gtgccctcga cgacaaaatt
     2461 gccgccaacg agcgcgtcat cgcggccgct gaaagcttga tgatcgccat cgtggagtcc
     2521 gtctccgagt gtgcgccgct gtccacgctg gccggccggt cgacgacgct tctgcggccg
     2581 cgggagttcg acgacgtggt agcacacttc agccttccgg cgttcgacga cggagcgaag
     2641 ccccgactcg tcgccgccgg agccgtgaag agcggaaagt tccgcttgac cgggccctgc
     2701 gtattggtcg ccaagctgaa tcccaggatt ccgcgcatct gggatgtagc gagcctgccg
     2761 ccacagatgg cattggcgag cacggagttc gtggtgctga ggccgatcgg tctcgataca
     2821 tcggcgctgt ggtcagcgct gcggcaacct gatgtcgtgg agacgctgca gcagtgggtc
     2881 gccggcacct cggggagtca ccagcgggtg cagccgcggg acctgcttca gcttcgcgtc
     2941 cgggatgtcc gacgtctggg tgccgcgcag tcgcggacga tcgccggcct gggtgcgttg
     3001 tgtcatgcgc gtcgcgccga atgcgtgcgg ctgtccgcgt tgcgcgatgc cctgctcccg
     3061 ctgctgatat cgggaagggt tcgggtcgcg cgctgagact gcgctcacgg gcttgatagc
     3121 caggccgtcg gattgcccgg ctcctcagcg tggctcgctc ggaggtttag ggtcgccggg
     3181 ctgggtcgga ttgcccggct cctcggcgtg ccccgcatcg tcgccgggct gggtgggatt
     3241 gcccggctcc tcagcgtgtc ccgcatcgtc gccgggctaa ggtgagcgcc gtggccgaga
     3301 ccgcgccgct gcgcgtgcag ctgatcgcca aaaccgagtt catggcgccg ccggaggtgc
     3361 cgtggacgac cgacgccgac ggcggcgccg cgctcgtcga gttcgccggc cgggcctgct
     3421 atcagagctg gtcaaagccc aatcccaaga ccgcgaccaa cgccggctac atcaagcaca
     3481 tcatcgacgt cggacacttc tcggtgctgg agcatgcgag cgtgtcgttc tacatcaccg
     3541 gcatctcgcg atcctgcaca cacgagctga tccggcatcg gcatttctcg tattcgcagc
     3601 tgtcgcaacg ctatgtgccg gaggggaaat cgcaggtcgt cgtcccaccg ggcatggagg
     3661 acgacccaga actgcagcag atcctgaccg cggccgccga cgccagccgc gccagctaca
     3721 ccgagctgct ggccaagctg gaagccaagt tcgctgacca acctaacgcc gtgctgcgcc
     3781 gcaagcaggc gcgtcaagcc gcccgcgcgg tgctgcccaa cgccaccgaa acgcgcatcg
     3841 tcgtgaccgg caactaccgg gcgtggcggc acttcatcgc catgcgggcc agcgagcacg
     3901 ccgacgtgga aatccggcgc ctggccatcg agtgtctgcg ccagctcgcg ggcgtcgcgc
     3961 ccacggtgtt cgccgacttc gaggtctcca cgctggccga cggtaccgag gtcgccacca
     4021 gtcctttggc tacggaggcc tgaaccgcgc gcgtgcacgg atgcacggac ttctcggcgt
     4081 gtcgcggtac caacacgcac gctcggcggc tgccaaatgc ccttgccccg ccaggtaacc
     4141 tgtgaaccgt gaccaccgtc ggattcgacg cccccgcccg cctgggaacc ctgctgactg
     4201 cgatggtgac accgtttggc gcagatggct ccctggacac caccgccgca gggcggctgg
     4261 cgaagcacct ggtagacggg gggtgcgacg gcctggtggt ctcggggacc accggtgagt
     4321 cgccgaccac cagcgacgac gagaagcgcg agctggtgcg cgtcgtgctg gaggcggtcg
     4381 gcgacagggc ccgcgtcatc gccggcgccg gcacctacga caccgcccac agcatccggc
     4441 ttgccaaggc cgctgcagcc gagggcgcgc acggtctgct cttggtcacc ccgtactact
     4501 cgaagccgcc gcaaagcgga ctgcgcgcac acttcacggc tgtcgccgac gcgaccgagc
     4561 tgccggtact gctctacgac atcccgggac ggtcggggac agcgatcgag ccggacacga
     4621 tccgcgcgct ggcgtcgcat ccgaacatcg tcggcgtgaa ggaggccaaa gccgacctgc
     4681 acaatagcgc gcagatcatc gccgagacgg ggctggccta ctactcgggc gacgacgccc
     4741 tgaacctgcc ctggctcgcg atgggggcca ccggcttcat cagcgtgatc gctcatctgg
     4801 cgtcgggtca gctacgggaa ttgttgtccg ccttcaattc cggcgatatc ggcaccgccc
     4861 ggaagatcca cgtcgcggtc gccccgctgt gcaacgcgat ggcccgcttg ggcggggtga
     4921 cgatgtcgaa ggcgggtctg cgcctgcaag gcatcgacgt cggcgatccc cggctgccgc
     4981 aaatgcccgc gacgcgggag caaatcgacg cgctggccgc cgacatgcgc gcggcctcgg
     5041 tgctgcggtg agccagaggg tgagccggac gtgaacgaag acctcgcccc gccaggtcct
     5101 ttggcgccag gcgggttgcg ggtcaccgcg ctgggcggga tcagcgaaat cggccgcaac
     5161 atgacggttt tcgagcacct cggtcgcctg ctgatcatcg actgcggagt gatgttcccc
     5221 acccacgacg agccgggcgt cgacctgatc ttgccggacc tgcgccatat cgaggaccga
     5281 ctcgacgacg tcgaggcgct ggtgctcacc catgcgcacg aggaccacat cggggccatc
     5341 cccttcttgc tcaggctgcg gccggacatc ccggtcgtcg gatcgaggtt caccctggcg
     5401 ctcgtcgccg cgaaatgccg cgagtaccgc atcaagccgg tgttcgttca ggtcgccgag
     5461 ggacaacaca gcacgcacgg cgtgttccag tgcgaatact tcgccgtcaa ccactcgatt
     5521 cccgacgcgc tggccatcgc ggtccacacc ggcgcgggaa ccgttttgca caccggcgac
     5581 atcaagctcg accagcttcc gcccgacggc cggcccaccg acctgcccgg catgtcccgg
     5641 cttggcaacg cgggtgtgga cctgttcctg tgcgactcga ccaattccga gatccccggt
     5701 gtcgggccgt cggaaagcga agtcggtccg agtttgcacc ggctgatccg cggcgccgac
     5761 ggtcgcgtca tcgtggcgtg tttcgcctcc aatgtcgacc gggtgcaaca gatcatcgac
     5821 gccgccgtgg cattgggccg ccgagtgtcg ttcgtcgggc ggtcgatggt gcgcaacatg
     5881 ggcatcgccc gggaactggg tttcctgcgg gtggacgatt ccgacctcat cgacatctcc
     5941 gccgccgaga tgatggcacc cgatcaggtg gtgctgatca ccaccggcac ccagggcgag
     6001 ccgatggcgg cgttgtcacg gatgtcgcgc ggcgaacacc gcagcatcac gctgacggcg
     6061 gaggatctgg tgatcctgtc gtcgtcgctg atacccggca acgaggaagc ggtatatggg
     6121 atcatcgact cgctggccaa ggtcggggcc cgcatcgtca ccaaccagca agtgcgcgtt
     6181 catgtttcgg gtcacgcata cgcgggcgag ctgcttttcc tctacaacgg ggtgcgtccg
     6241 cgcaacgtga tgcccgttca cggcacctgg cggatgctgc gcgccaacgc caagctggcc
     6301 gcgcgcgccg gggtgccgga ggagtcaatc ctgttggccg agaacggtgt cagcgtcgac
     6361 ctggtcgccg gcaaggccgc catcgccggg gcggtgccgg tcggcaagat gttcgtcgac
     6421 ggactgatca ccggcgacgt cggcgatatc accttggggg agcggctcat cttgtcgtcg
     6481 ggtttcgtcg cggtgaccgt ggtggtcaag cgtggcaccg gcctcccggc ggttgtgccg
     6541 caactgcttt cacgcggctt ctccgaagac cccaaagcac tcgagccggc ggtgcgcaag
     6601 gtcgaggcgg agctggagca gctggccgcc gcgggcgtca ccgatccggt gcgcatcgca
     6661 cagggcgtgc gccgcatcgt cggcaagtgg gtcggcgaaa cctatcgcag gcagccgatg
     6721 atcgtgccga ccgtcttgga agtttgagtc gctagcttga attgcccgac tcgccccctt
     6781 gccgctgcgc ggctgggggt acccccacct catacggcta cgccgtatgc atcgtcgtcg
     6841 ggctagatct tctccgcgta agccgaccat tccagctgcg agcctttgag tttgcggccg
     6901 gtgggcgcga cgtagcggcg catgagttc
//
LOCUS       DASSGQ010000361        11167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1456060,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000361 DASSGQ010000000
VERSION     DASSGQ010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11167
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456060"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFI55_16070"
     CDS             <1..322
                     /locus_tag="VFI55_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075581.1"
                     /translation="ADHGALQKQLDHGHRWPVPIKKSELRVEHEPSLPLTAVPVQKLG
                     AICFDPYPQPTGIAGCPNVGGAQLAPYVRTSFDMEIALHWSTTWTEKPTDLAALLDRL
                     GRHV"
     gene            714..2492
                     /locus_tag="VFI55_16075"
     CDS             714..2492
                     /locus_tag="VFI55_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7075582.1"
                     /translation="MEGTPFGRYRLVELLGHGGTGEVWRAFDTATDRVVALKVLPANF
                     ADDQRFRERFRREATAAAGLVEPHVIPIYDFGEIEGRLFVNMRLIQGRDLRSLLGRGS
                     LVPHRAVGIVGQIAAALHAAHQIGLVHGDVRPSNILVADDDVADDDVAYLIDFGIARV
                     AAETEPAGAGASISAWAYMAPERFQKGAVDARADIYALACVLHECLTGQPPFAGDTLE
                     QIAFAHMRQPPPRPSELRDGIPAAMDQVVAVGLAKDPEQRYATTEDLAHAARAVLPTV
                     VRQTSRSTPSATRVRVAATPPEQPARADMAQGDASTTSGGGSVTASADTAKATEIEES
                     IPDASVLQDVHTAAKVSMGAAPPPASAGNGRPGEPGSTLEATPSTEQFVFQRQDRRHR
                     VAAGAAALFLVVVGIAVVVIVLGNRPPSGAGSASSTSTTDVTGPSPPASPMSTESTTP
                     SISPPDRLRAMLPTDLRCKVDTGRFADAPVSIAQAVCPYGGPDLIRYWLFADKDTMYR
                     SFNKLNKGLAETFVACPGMKEGPQDWQNAADPQQSGKVTCSVFPSPVNFIRWTIDSQL
                     VMGSIGGEHYGQTSDQLLQWWRAHYE"
     gene            2625..4982
                     /gene="pcrA"
                     /locus_tag="VFI55_16080"
     CDS             2625..4982
                     /gene="pcrA"
                     /locus_tag="VFI55_16080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HET7075583.1"
                     /translation="MSVHATSNTEAKSDPEVDRLLDDLNPQQRQAVVHEGSPLLIVAG
                     AGSGKTAVLTRRIAYLIAARGAGVGQILAITFTNKAAAEMRERVVRLVGNRARAMWVS
                     TFHSTCVRILRNQASLIKGLNSNFSIYDADDSRRLLQMIGRDMGLDIKRYSPRLLANA
                     ISNLKNELIDPDQALANLANLSDASDELSRTVASVYGEYQRRLRTANALDFDDLIGET
                     VAVLQAFPQIAQYYRRRFRYVLVDEYQDTNHAQYVLVRELVGRGSTDSQETSDEVPPA
                     ELCVVGDADQSIYAFRGATIRNIEDFERDYPDATTILLEQNYRSTQNILSAANSVIAR
                     NTGRRAKRLWTDAGAGELIVGYVADNEHDEARFVAEEIDALAENGDITYNDVAVFYRT
                     NNSSRSLEEVFIRAGIPYKVVGGVRFYERKEIRDIVAYLRVLDNPGDAVSMRRILNTP
                     RRGIGDRAEACVAVYAENTGASFADALVAGAQGKVAMLNTRAEKAIAGFVELLDDLRG
                     HLADDLGELVELVLERTGYRRELESSTDPQELARLDNLNELVSVAHEFSTDQALDTSE
                     AAALAEDNEDVPDTGVLAAFLERVSLVADADEIPEHGAGLVTLMTLHTAKGLEFPVVF
                     VTGWEDGMFPHMRSLDDPTELSEERRLAYVGITRARHRLYVSRAIVRSSWGQPMLNPE
                     SRFLREIPQELIDWRRSAPVSSFSAPVSGAGRFGAPRPAPARSAAGKRPLLVLQPGDR
                     VTHDKYGLGRVEEVSGVGESAMSLIDFGSSGRVKLMHNHAPVSKL"
     gene            complement(5101..5682)
                     /locus_tag="VFI55_16085"
     CDS             complement(5101..5682)
                     /locus_tag="VFI55_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075584.1"
                     /translation="MTAPNDPPAPPNPEPAAPPSESRTRIIRRAPTGPVPIVPESPTT
                     HILRQHPPYQGPPAAVRRGTSSALSSDEPSDRTAVAACAVSIVSGWATSVVATDLIAG
                     WWRTDRLFCIAVAFLALVFSVTTVTGVIMLLQRRALGRYLIVAGAAVAVLTYGGVFIA
                     GARVAWIVHALPVLPIASVVLAMHPRTTRWLEG"
     gene            complement(5684..6778)
                     /locus_tag="VFI55_16090"
     CDS             complement(5684..6778)
                     /locus_tag="VFI55_16090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020786107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET7075585.1"
                     /translation="MPQHRFARSSALPRTVRAHREHWQPRHHNEVTEILPLDGFDFDD
                     LDFVDDFADLDGLDFSNDSTFDNEAQVLLAPELDDLAEIDNLTPQWLTAPTVVLARVP
                     AEPDLRLEKLEAAHALDVTDVIPAVRRGGQHRKQPTSAARGRLLIGAMAAGAAAAAAH
                     TATSHADTPKTEAVLTANASALTGGAGSNTIRGAQVVALQPAANGAVHNEELEKGMAF
                     ANDRAQREARLQQPLYVMPTHGIFTSNFGYRWGVLHAGIDLANSIGTPILAVSDGVVI
                     DAGPTAGYGMWVKLRHADGTVTLYGHVNTTLVSVGQSVMAGDQIATMGNRGNSTGPHL
                     HFEVLQGGTERIDPVPWLAKRGLSVGNYAG"
     gene            7042..8205
                     /gene="sucC"
                     /locus_tag="VFI55_16095"
     CDS             7042..8205
                     /gene="sucC"
                     /locus_tag="VFI55_16095"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HET7075586.1"
                     /translation="MDLFEYQAKELFVKHDVPSSPGRVTDTAEGARAIAEEIGRPVMV
                     KAQVKVGGRGKAGGVKYAATPDDAYEHAKNILGLDIKGHIVKKLLVAEASDIAEEYYI
                     SFLLDRANRTYLAMCSVEGGMEIEEVAATKPDRLAKVAVDAVKGVDLATARSIAERGH
                     LPAEVLDAAAVTISKLWDLFVGEDATLVEVNPLVRTPDNQILALDGKVTLDNNADFRH
                     PDHAEFEDRDATDPLELKAKEHDLNYVKLDGQVGIIGNGAGLVMSTLDVVAYAGEKHG
                     GVKPANFLDIGGGASAAVMAAGLDVILGDEQVKSVFVNVFGGITACDAVATGIVKALE
                     ILGDEANKPLVVRLDGNNVEEGLRILNDANHPLVTLVATMDEAADKAAELASA"
     gene            8271..9173
                     /gene="sucD"
                     /locus_tag="VFI55_16100"
     CDS             8271..9173
                     /gene="sucD"
                     /locus_tag="VFI55_16100"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HET7075587.1"
                     /translation="MSIFLNKDNKVIVQGITGGEATVHTARMLKAGTQIVGGVNARKA
                     GTTVTHENKDGQAVELPVFGSVAEAMEKTGADVSIIFVPPKFAKDAIIEAVDAEIPLL
                     VVITEGIPVQDTAYAWAYNLDKGGKTRIIGPNCPGIISPGQALVGITPANITGAGPVG
                     LVSKSGTLTYQMMYELRDFGFTTSIGIGGDPVIGTTHIDAIEAFEKDPDTKIIVMIGE
                     IGGDAEERAADFIKANVSKPVVGYVAGFTAPEGKTMGHAGAIVSGSSGTAAAKKEALE
                     AAGVKVGKTPSETAALAREILKSL"
     gene            complement(9402..10730)
                     /locus_tag="VFI55_16105"
     CDS             complement(9402..10730)
                     /locus_tag="VFI55_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HET7075588.1"
                     /translation="MSYVIAAPEFVTAAASDLANIGSAVSQANAAASVQTSSVLAAGA
                     DEVSAAIASLFGAHGQAYQALSAQATDFHQQFVQLMTAGAGQYASTEAANANPLVAIE
                     QQLLGVINTPTQLLLGRPLIGNGANAAAGSGANGGDGGLLWGNGGAGGSASTSHLTGG
                     NGGAAGLFGNGGPGGVGESGGGRGGNGGAGGLFFGTGGAGGAGGGVFGASAAGLGGNG
                     GAAGLFGVGGAGGAGGVSNHTDVVGGTGGAGGSGGLIGTGGAGGRGGSGGGAGGLGGT
                     GGAGGSGGILFGNGGIGGAGGDTPLQAAGNGGNGGDGGFLYGQGGAGGAGGSDPILAG
                     GGGNGGPGGNGGKVIGSGGEGGAGGLGQPGGNGGAGGNAGILVGNGGAGGAGGDVSGH
                     GNNGGNAGLIGNGGNGGNGGNGGIVGGPGGQGGSALLFGADGTPGTPGAT"
     gene            complement(10928..>11167)
                     /locus_tag="VFI55_16110"
     CDS             complement(10928..>11167)
                     /locus_tag="VFI55_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HET7075589.1"
                     /translation="GGTGGVGGDAGLLFGNGGAGGSGGSATTGGAGGNGGRAALLIGF
                     GGNGGNGGAGTTTSGPGGQGGDGGQLIGVPGKPGT"
BASE COUNT         1979 a         3666 c         3672 g         1850 t
ORIGIN      
        1 agcggaccac ggggctctgc aaaagcagct agaccacggg caccgctggc cggtacccat
       61 aaaaaaatcg gagctgcgcg tcgagcacga accgagtctg ccactaacgg cagtgccggt
      121 gcaaaaactg ggtgcaattt gcttcgatcc ctaccctcag ccgactggca tcgccggctg
      181 cccgaatgtc ggcggtgcgc agctcgcgcc atacgtgagg acatcctttg acatggaaat
      241 cgccttgcac tggtccacca cctggacgga gaaaccgact gacctcgccg cccttctcga
      301 ccggctcgga cgacatgtgt aagcggcccc caccgccgtt tcagggtcgc gcgccggtag
      361 tgggacctcc tgccatcggc accatgactg cccaagtccg agatgtcctc agcgaccacc
      421 aatcgctcgc ccgcacgggt gaggtgatta ccagcgctga gatggcgcca accgcgtacg
      481 accaaatcga ccgggcccga acagaactga acgccgtctc gaaacagacg acgtacgatg
      541 gtctcgagac ttcgcatcag actggcggac atcgacggca gcagtgttcg tatctgtagc
      601 cgtcggtcgg atgtcgcccc gcttgccggg cttaagggcg atcggctgcg ggggcgtcag
      661 caatagcgat atcgtgcttg caaattgctg gcctttcgct ttaggaggct tgggtggagg
      721 gcacgccttt cggtcgctac cgtctcgtgg agttgctagg ccacggcggc acgggcgagg
      781 tgtggcgcgc attcgacacc gccaccgacc gggtggtggc tcttaaggta ctgcccgcga
      841 acttcgcgga cgaccagcga ttccgggagc ggtttcgacg cgaggccacc gcagcagcgg
      901 ggctcgtcga gccccatgtc atcccgattt acgacttcgg cgaaattgag ggccgtttgt
      961 ttgtgaacat gcggttgatc cagggccggg acctgcgaag cttgctgggc cgaggttcac
     1021 tcgtaccaca ccgggcggtg gggattgttg gccagatcgc cgcggcgctg cacgccgcgc
     1081 accagatcgg gctggtgcac ggcgacgtta ggccgtccaa catccttgtt gctgacgacg
     1141 acgttgccga cgacgacgtc gcttacctga tcgactttgg gattgctcgt gtcgccgcag
     1201 agaccgagcc tgcgggcgcc ggcgcgtcta tcagcgcctg ggcctatatg gcccccgaac
     1261 ggttccagaa aggcgccgtg gacgcacgtg ccgatatcta cgcgctggcc tgcgtgctgc
     1321 atgagtgcct gaccggccag ccgccctttg cgggtgacac ccttgagcag atcgccttcg
     1381 ctcacatgcg tcagccaccc ccacgaccat cggagctgcg ggacgggatt cctgcagcga
     1441 tggaccaggt cgttgctgtt ggactagcca aagaccccga gcagcgctac gccacgaccg
     1501 aagacttggc gcacgcggcg cgggccgtat tgcctacggt ggttcgacag actagccgat
     1561 caacaccatc cgccacccgt gtccgcgtcg ccgctacacc cccagagcag ccggctcgcg
     1621 cagatatggc ccagggcgac gcgtcgacaa cttcgggtgg aggatcggtg accgcatctg
     1681 cggacaccgc gaaggccacc gagatcgaag agtcgatacc cgatgcgtca gtgctccaag
     1741 acgttcacac cgcggccaaa gtaagcatgg gggcagcgcc gccgcccgcc tcggctggga
     1801 acgggcgccc tggggagccc ggatcgacct tggaagcgac tccatccacc gagcagtttg
     1861 tgtttcagcg gcaagaccgt cgccataggg tcgctgctgg tgcggcggct ctgtttttgg
     1921 ttgttgtggg gattgcagtg gtcgttatcg ttcttggtaa tcggccaccg tcgggtgctg
     1981 gtagcgcgtc ttcgacgtct acgacggatg tcactgggcc cagcccgccg gcatcgccga
     2041 tgagtaccga gtcgacgaca cccagtatta gcccgcccga ccggttgaga gccatgcttc
     2101 ccactgacct gcggtgcaag gttgacacgg gtaggttcgc cgatgcaccc gtatcgatag
     2161 cacaggcagt gtgcccttat ggaggcccag atctgatccg ttactggctc tttgccgata
     2221 aggacacgat gtaccgctcc ttcaacaaac taaacaaagg gctcgccgag actttcgtcg
     2281 cctgtcccgg aatgaaggag ggaccgcagg actggcaaaa cgcagcggat ccgcaacaaa
     2341 gcggcaaagt cacgtgttca gtcttcccct ctcccgtgaa cttcattcgc tggacgatcg
     2401 actcgcagtt ggttatgggc agcattggcg gggaacatta cgggcaaacc tccgaccagc
     2461 tattgcaatg gtggagggct cactacgaat agcgaatcgg cagccgactt gccttgcctg
     2521 aacgcccctg aatttgggct tttacgcaga ggtgcggtgc taaaagggcc ggctgcgcgg
     2581 gcgcaaccgt cgtcgtcggc gggcggcggt aaatttggac cgacatgagt gtgcacgcaa
     2641 ccagtaatac tgaagccaag tccgaccccg aagtggatcg actgctcgac gacctcaacc
     2701 cgcaacagcg ccaggcggtg gtgcatgagg gttcgccgct gctgatcgtg gccggcgccg
     2761 gctcggggaa gacggcggtg ctgacgcggc gcatcgcgta cctgatcgcg gcgcggggcg
     2821 ccggggtggg ccagattctg gcgatcacct tcaccaacaa ggccgccgcg gagatgcgcg
     2881 aacgggtggt acggctggtg ggcaaccgcg cccgcgcaat gtgggtatcc acgtttcact
     2941 cgacgtgtgt gcgcatcctg cgcaaccagg cgtcgctgat caaaggactc aactccaact
     3001 tttcgatcta cgacgccgac gattcgcggc gtctgctcca gatgatcggc cgcgacatgg
     3061 ggctggacat caagcgctac tcgccgcgcc tgctggccaa cgcgatctcc aatttgaaga
     3121 acgagttgat cgaccccgac caggccctag ccaacttagc caacctgtcg gacgcttccg
     3181 atgagttgag ccgcaccgtt gcgtcggtgt acggcgaata ccagcggcgg ctgcggacgg
     3241 ccaatgcgct ggacttcgat gatctgatcg gcgaaaccgt cgcggtgcta caagccttcc
     3301 cgcagattgc ccagtattac cggcgccggt tccggtacgt cctggttgac gagtaccaag
     3361 acaccaacca cgcccagtac gtgctggtgc gcgaactggt cggtcggggc agcacggatt
     3421 cgcaagagac atcggacgaa gtgccgccgg cggagttgtg tgtggtcggc gatgccgacc
     3481 agtcgatcta cgcgttccgc ggcgccacca tccgcaacat cgaagacttc gagcgcgact
     3541 accccgacgc gacaaccatt ctgctggaac agaattaccg ctcgacgcaa aacatcctgt
     3601 cggccgccaa ctcggtgatc gcccgtaaca ccggacgccg tgcgaaacgc ctgtggaccg
     3661 acgcaggggc cggggagctg attgtcggct atgtcgccga caacgagcat gacgaggcca
     3721 ggttcgtcgc agaggagatc gacgccctcg ccgagaacgg cgatatcacc tacaacgacg
     3781 tggccgtgtt ctaccgcacc aacaactcgt cgcggtcgtt ggaggaggtc ttcatccgcg
     3841 ccggaattcc gtacaaggtc gtcgggggag tgcgcttcta cgagcgtaag gagattcgcg
     3901 acatcgtcgc ctatctgcgg gtgctggaca accccggcga cgcggtcagc atgcggcgca
     3961 tcctcaacac accgcgtcgc ggcatcggcg atcgcgccga ggcttgtgtg gcggtgtacg
     4021 ccgaaaacac cggcgccagt ttcgccgacg cactggtggc cggggcgcag ggcaaagtcg
     4081 cgatgctgaa tacccgcgcg gagaaggcga ttgcgggctt tgtcgagctg ctcgacgacc
     4141 tgagggggca cctcgccgac gatctcggtg aactagtgga gttggtgctc gaacgcacgg
     4201 gataccgccg tgagttggag tcctccaccg atccgcagga attggcccgg ctggacaatc
     4261 tcaacgaatt ggtcagcgtc gcacacgaat tcagcaccga ccaggccctt gacaccagcg
     4321 aagcggccgc gcttgctgag gacaacgaag acgtgccgga caccggcgtg ctggcggcgt
     4381 tcctggagcg ggtgtcattg gtcgccgacg cagacgaaat cccggagcac ggcgccgggc
     4441 tggtcaccct gatgacgctg cacaccgcga agggtctgga gtttccggtg gtgtttgtca
     4501 ctggctggga ggacgggatg ttcccgcaca tgcggtcgct ggatgatccc accgaactct
     4561 ccgaagagcg gcggctggcc tacgtcggca tcacccgggc ccgtcaccgg ctgtatgtga
     4621 gcagggccat cgtccggtct tcgtgggggc agccgatgct caaccccgaa tcgcggttcc
     4681 tgcgcgagat cccccaggag ctcatcgact ggcggcgcag tgcgcccgtg tcgtcgttca
     4741 gcgcgccggt cagcggtgcc ggccggttcg gcgcgccgcg tccggcgccg gcccgctcgg
     4801 cggcgggcaa acgtcccctg ctggtgctgc agcccggcga tcgggtgact cacgacaagt
     4861 acgggctggg ccgcgtcgaa gaggtctccg gggtgggcga atcggcgatg tcactgatcg
     4921 acttcggcag ttcgggacgg gtgaagctga tgcacaacca cgccccggta agcaagcttt
     4981 agcccggcga cgatgcgggc cgcatcgcgg cccgtggtgg gggtaccgcc cgcttacggg
     5041 ggagagctgg gcgatcgagc tttagcgctg ggcaatcagg tttagcgcgg cgagcccggc
     5101 ttatccctcc agccagcgtg tggttcgcgg gtgcatcgcc agcaccacac tggcgatcgg
     5161 tagcaccggc agggcgtgca cgatccacgc gacgcgcgct ccggcgatga acacgccacc
     5221 gtacgtcagc accgcgaccg cagcgccggc cacgatgagg tagcgcccga gggcccggcg
     5281 ctgcagcaac atgatcacgc cggtgacggt ggtgaccgaa aacaccaggg cgagaaaggc
     5341 gaccgcgatg cagaacaggc ggtcggttcg ccaccacccg gcgatcaggt cggtggccac
     5401 caccgacgtc gcccatccgc tcacgatgct gaccgcgcaa gcggcgacgg cggtccggtc
     5461 actcggctca tcgctcgaca gagccgagct ggttccccgc cggaccgctg ccgggggacc
     5521 ctgatagggc ggatgctgcc ggagaatgtg agtggttggg gactcaggca ctatgggcac
     5581 cggcccggtc ggtgcccgcc ggatgatccg agtccgagat tccgacggcg gcgcagcagg
     5641 ctcggggttg ggaggagcag gggggtcgtt cggcgcggtc acttcagcca gcgtagttgc
     5701 cgacgctgag gcctcgcttt gccaaccacg gcacggggtc gattctttcg gtgccgccct
     5761 gcagcacctc gaaatgcaga tgcgggccgg tcgagttgcc acggttgccc atggtggcta
     5821 tctggtcgcc ggccatcacg ctctggccga cgctgaccag cgtggtgttg acgtggccgt
     5881 agagcgtgac cgtgccgtcg gcatggcgca acttgaccca catcccgtag ccggcggtcg
     5941 ggcccgcgtc gatgactaca ccgtcggaga cggcgaggat cggcgttccg atcgagttgg
     6001 ccaggtcgat accggcgtgc agcacacccc agcgataccc gaagttggag gtgaagatcc
     6061 cgtgggtggg catcacatac agcggctgct gcagtcgtgc ctcacgctgc gcgcggtcgt
     6121 tggcgaaggc catccccttt tccaactcct cgttgtgcac cgctccgttc gccgcgggct
     6181 ggagcgcgac cacctgggcg ccgcggatcg tgttgctgcc ggcgccgccg gtgagcgccg
     6241 acgcgttggc ggtcaggacg gcctcggtct tcggggtatc ggcatggctg gtggccgtgt
     6301 gtgcggccgc agccgccgcc ccggccgcca tggccccgat cagcagtcgg cctctggccg
     6361 cgctggtggg ctgcttgcga tgctgaccgc cacgccgcac cgctgggatg acgtcggtga
     6421 cgtcgagagc atgagccgcc tcgagcttct ccagcctgag gtccggctcg gcgggtacac
     6481 gggccagaac tacggtggga gcggtcaacc actgcggcgt cagattgtcg atctcggcca
     6541 aatcgtcgag ttcgggggcc aacagcactt gtgcttcgtt gtcgaaggtc gagtcgttgc
     6601 tgaagtccag gccgtcgagg tcggcgaaat catccacgaa gtcaaggtcg tcgaagtcga
     6661 atccgtcgag cgggagaatt tcggtgactt cgttgtgatg gcgcggttgc cagtgctcgc
     6721 gatgggcgcg aacggtcctc ggcagggccg aagaacgagc gaaacggtgc tggggcaagc
     6781 tgaattgtcc tcgtatcgtg accataacgt tatctggacc ctaggaaggt agccgttgac
     6841 cgccagaact ggcaacttgg ggccaaaatc cgcccgacat tgtgatttgg atcacacctg
     6901 ccgtatgcct gttctgtcca acatggtgga cgcgacggta gcagtgattc ggtgggtgga
     6961 tacagtgcca gcgtgcgagt tgccgattcc ggcaccgcca acggataggc ctaccagcaa
     7021 gtcgacgaag acagtgagcc catggacctt ttcgagtatc aagcaaaaga attgttcgtc
     7081 aagcacgacg tgccgagctc gcccggtcgg gtgaccgaca ccgccgaggg cgctcgggcc
     7141 atcgccgaag agatcggtcg cccggtgatg gtcaaggcac aagtcaaggt cggcggtcgt
     7201 ggcaaggccg gtggtgtcaa gtacgccgct actccggatg acgcctacga gcacgccaag
     7261 aacatcctcg gcctggatat caagggccac atcgtgaaga aactgctggt cgccgaggcc
     7321 agcgacatcg ccgaggagta ctacatctcc ttcctgctcg accgtgccaa ccgcacctac
     7381 ctggcgatgt gctcggtcga gggtggcatg gagatcgaag aagtggccgc caccaagccc
     7441 gaccggctcg ccaaggtcgc ggtggacgcc gtcaagggcg tcgacctggc gaccgcgcgt
     7501 tccatcgccg agcggggtca cctgccggcc gaggtgctcg acgccgccgc ggtgaccatc
     7561 tccaagctgt gggacctgtt cgtcggcgag gacgcgacgc tggtcgaggt caatccattg
     7621 gtgcgcactc ctgacaacca gatcctggcg ctggacggca aggtcaccct cgacaacaac
     7681 gccgacttcc gccaccccga tcacgccgaa ttcgaggacc gcgatgccac cgacccgctg
     7741 gagctgaagg ccaaagagca cgacctgaac tacgtcaagc tggacggcca ggtcggcatc
     7801 atcggcaacg gcgcgggtct ggtgatgtcg acgctcgacg tcgtcgcgta tgcgggggag
     7861 aagcacggtg gagtcaagcc cgccaacttc ctcgacatcg gtggcggcgc ctcggcggcg
     7921 gtgatggccg cggggttgga cgtgatcttg ggtgacgaac aggtcaagag tgtgttcgtc
     7981 aacgtcttcg gaggcatcac cgcctgtgac gcggtggcca ccggaatcgt caaggcgctg
     8041 gagatccttg gcgatgaagc caacaagccg ctcgtggtgc ggctggacgg caacaacgtc
     8101 gaggaaggcc ttcggattct gaacgatgcc aaccacccgc tggtgacgct ggtggcgacc
     8161 atggacgagg ccgccgacaa ggccgccgag ctggcgagcg cctgacgtgg cgatcgcaag
     8221 agcggcgaag ccggtcgtag cgggtcgaca ctaagaaagg acttgtggaa atgtcgatct
     8281 ttttgaacaa ggacaacaag gtcatcgtcc agggcatcac cggcggcgag gccaccgtcc
     8341 acaccgcccg aatgctcaag gcgggcaccc agatcgtggg cggtgtgaac gcacgcaagg
     8401 cgggcaccac cgtcacgcat gagaacaaag acggccaagc cgtcgagctg ccggtgttcg
     8461 gcagcgtcgc ggaggcgatg gaaaagaccg gcgccgacgt gtcgatcatc ttcgtgccgc
     8521 cgaagttcgc caaagacgcc atcatcgagg ccgtcgacgc cgaaataccg ctgttggtgg
     8581 tgatcaccga gggaattccg gtgcaggaca ccgcatacgc ctgggcctat aacctcgaca
     8641 agggcggcaa gacccgcatc atcggcccga actgccccgg catcatcagc cccggccagg
     8701 cgctggtcgg catcaccccg gccaacatca ccggcgcggg ccccgtcggg ctggtgtcca
     8761 agtcggggac gctgacctac cagatgatgt acgaactgcg cgatttcggg ttcaccacat
     8821 cgatcgggat cggcggcgac ccggtgatcg gcaccaccca tatcgacgcc atcgaggcgt
     8881 tcgagaagga tcccgacacg aagatcatcg tgatgatcgg cgagatcggc ggcgacgccg
     8941 aggagcgggc ggcggacttc atcaaggcca acgtgtccaa gccggtcgtc ggctacgtcg
     9001 cgggattcac cgcaccggaa ggcaagacca tgggacacgc gggcgccatc gtgtcggggt
     9061 cgtcgggcac cgcggccgcc aagaaggaag ccttggaagc ggccggcgtc aaggtcggca
     9121 agacgccgtc ggagacggcg gcgctggccc gggagatcct gaagagcctg taacggctgg
     9181 cggttgcgct gagcggtggc ggtgttcagc cttagatgcc gccaccgtcg ccgcagttgg
     9241 ccatcagttt ggcgttgccg ccctcgtcgg cggcccgcag tggaggcctt cgccgggcgc
     9301 cggatcggag ctcccgcagc gacggtgtcg aggtcgagaa ggtgccgccg gattttgcgt
     9361 ctcagttgcg attaaaggcg ggccctggct cggcccttga tttaggtggc ccccggggtc
     9421 ccgggcgtcc catccgcgcc gaatagcaac gccgagccgc cttgcccacc tgggcccccg
     9481 acgatgccgc cgttgccgcc gttgccgccg ttgccgccgt tgccgatcag ccccgcgttg
     9541 ccaccgttat tgccatgccc gctgacgtcg ccgccggcgc cgccggcgcc accgttgcca
     9601 acgaggatgc cggcgttgcc gccggccccg ccgttaccgc ctggctggcc caacccgcct
     9661 gccccgccct cgccaccgga cccgatgacc ttcccgccgt tcccgccggg tccaccattg
     9721 ccgccgccgc cggccaaaat tgggtcgctt ccgccggcgc ctccggcgcc tccctgaccg
     9781 taaaggaatc cgccatcgcc gccgttgccg ccgttgccgg cggcctgcaa gggggtatcc
     9841 ccgcccgcac cgccgattcc gccgttgccg aacaggatgc cgccacttcc gccggccccg
     9901 ccggttccgc caaggccgcc agcgcctcct ccgcttccgc cgcggccacc agcgccaccg
     9961 gtgccgatca ggccgccgct gccacccgcg ccgccggtcc cgccgacaac gtcggtgtga
    10021 tttgagacgc cgccggctcc accggcgccc ccgacgccga acagtcccgc agccccgcca
    10081 ttgccgccga gtccggcggc tgaggcgcca aagacgccgc cgccggcccc gccggcgccg
    10141 ccggtgccga agaatagccc gccggctccg ccgttgccgc cccggccgcc gccggattcg
    10201 ccgacgccgc ccggcccgcc gttgccgaac aacccggcgg cgccaccgtt accgcccgtc
    10261 aggtgggacg tgcttgcgga cccgcctgcg ccgccattgc cccacagcaa tccgccatcc
    10321 ccgccgttgg ccccagagcc cgccgccgcg ttggcgccgt tgccgatcag cgggcgtccc
    10381 aaaagcagtt gggtgggcgt attgatcacg ccgagcagtt gctgctcgat ggccaccagc
    10441 gggttggcat tggcggcctc ggtgctggcg tattgtcctg cacccgcagt catgagctgg
    10501 acaaactgct ggtgaaagtc cgtggcctga gcgctgagcg cctgataggc ctgtccatgc
    10561 gcgccgaaaa gcgaggctat ggccgccgat acctcgtcgg caccggctgc caacacgctc
    10621 gacgtctgga ccgaagctgc cgcgttggcc tggctgacgg ccgaaccgat gttggccaaa
    10681 tccgaagccg ctgctgtcac aaattccggc gccgcgatca cgtacgacaa ctgaagcctc
    10741 ccatataagt gatgggtatt caggcgtgac gatccggctt cgtcaggtcg gattgctgac
    10801 aaatcagtta gctgatcata tcgccatcac ttacaaacca ttgtgaattc aaggaaacaa
    10861 ccggtcccgc caggagacgc aaaagccccc gacacgccga gcgtgcgggg gcttttacgt
    10921 ctgctggtta cgtgccgggc ttgcccggca ccccgatcag ttgcccgccg tcgccgcctt
    10981 ggccgccggg gcccgaggtt gttgtcccgg ccccgccgtt gccaccattt ccgccgaaac
    11041 caattagtag tgcggcgcgt ccaccgttgc cgccggcgcc gccggtggta gcgctgcctc
    11101 cggagccgcc agcgccgccg ttgccaaaca gcaagcccgc gtcaccgccg acacctcctg
    11161 taccacc
//
LOCUS       DASSGQ010000362         6327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1395244,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000362 DASSGQ010000000
VERSION     DASSGQ010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395244"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..344
                     /locus_tag="VFI55_16115"
     CDS             39..344
                     /locus_tag="VFI55_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075590.1"
                     /translation="MNPGVDRITIARTAEPVPYWLECRSSERAVALIKIEFMGYQLRR
                     QVDLRPASRCTGKFELPEISLPKSIGGAISLNRELPAYASTATGRPESLSENRLFNI"
     gene            424..828
                     /locus_tag="VFI55_16120"
     CDS             424..828
                     /locus_tag="VFI55_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013829573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4267 domain-containing protein"
                     /protein_id="HET7075591.1"
                     /translation="MSIDRAALLAGSIRFASGVSFLVDPLRANRLWGDPEEPTPTARL
                     LLRSMGYRDALIGGLLVAAALRGKDTRGWFLASGGADAADLLGGMSVHRELKPAQRLV
                     GLGGAVVGVGVGLWGATRRTPSPAGPLTGGRS"
     gene            complement(858..2369)
                     /locus_tag="VFI55_16125"
     CDS             complement(858..2369)
                     /locus_tag="VFI55_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET7075592.1"
                     /translation="MTASVAVIGAGPAGLIAARWLLSQGFEPTIFERETMLGGQWAGL
                     DGRSGVWPSMHTNSSRVLTTFSDLEHDTDLVYPSNREILDYLRRYAETFGLASRIQFG
                     TRVELLSRGNRRDGSGWIVRHAGTDTRFERVVVATGRFHSPAIPYVPGLETFAGSAEA
                     KSTYHYRGASAYQGKRVLVAGCAISALEIASELAQLGTARVVLTQRRQRYVLPKFAAG
                     VPSDHRIYTRYGTLANETLPAAEIDRQLKEIVLASSGSPEQYGAPTPDPSLFVAGVTL
                     NQHYLPLVAEGRITVRPWMESIAGSTVAFGDGHAEEFDGIVFGTGFNLHLPFLDEDIQ
                     RILDLDAVHMDAHHYTFHPDLPGLAFMGMWDQSGGYFVPLELQARWVAYTWGGTISSP
                     TESNQRLAIKAYRSKRGTSQKTRMNLVAVNFARAAGVEPAVDNWPHLRRALLFGPLAP
                     SCFRLEGPDAAADAPTRFARDAAAFGAIVSNELTERERSQWSLVETASTRGQP"
     gene            complement(2486..3961)
                     /locus_tag="VFI55_16130"
     CDS             complement(2486..3961)
                     /locus_tag="VFI55_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HET7075593.1"
                     /translation="MSVTPDTTVALQHRFLRELPEMAVRWQADTAPDLRLLVLNERLA
                     SDLGLDSAWLQTSDGLRFLAGNLVPEGAAPVAQAYAGHQFGGFVPRLGDGRALLLGEL
                     VDDDGRMRDIHLKGSGPTPFARGGDGLAAVGPMLREYLVSEAMHALGVPTTRSLAVVA
                     TGRSVQRDTLLPGAVLARVASSHLRVGSFQYAASTGDINLLRRLADHAIARHHPGAAE
                     ADRPYVALFEAVVAAQASLIARWMLIGFVHGVMNTDNMTISGETIDYGPCAFMEAYDP
                     DTVFSSIDSWGRYAYGNQPAIAGWNLARFAESLLPLLADDVQDAIAIAEESFGVFGPE
                     YESVWSSGMRAKLGLSAHLGADVVTTLVDELLPLLRQSNVDYTSFFRHLGQAARRGAE
                     SDAEPARGLFIDLAGFDDWMSRWRALGPDAELMDRTNPIYIPRNHLVEEALTAATAGD
                     LDPLGTLLAAVTAPCDKRPGLERYASPAPEDFGTYQTFCGT"
     gene            4079..5014
                     /locus_tag="VFI55_16135"
     CDS             4079..5014
                     /locus_tag="VFI55_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HET7075594.1"
                     /translation="MTTDHAMYEHVDGPAADPADIGSRIDPVLARSWLLVNGAHADRF
                     QSSAHSRADIVVLDIEDAVAPKDKQAARDNVVRWLTADNTDWVRVNGFGTPWWADDLA
                     ALAGTPIGGVMLAMVESVDHVTETAQRLPNVPIVALVETARGLERITEIAAAKGTFRL
                     AFGIGDFRRDTGFGEEPTTLAYARSRFTIAAKAANLPSAIDGPTIGSNALKLIEATAV
                     SVEFGMTGKICLTPDQCAVVNEGLSPSQDEIAWAKEFFAEFERDGGEIRNGSDLPRIA
                     RATKILKLARAYGIEVSEFEDERVHSPAPTDTYHY"
     gene            complement(5022..5825)
                     /locus_tag="VFI55_16140"
     CDS             complement(5022..5825)
                     /locus_tag="VFI55_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7075595.1"
                     /translation="MTNAQSSHSAAEAASGSLAGKVAVITGAARGIGRQIGRVYAAHG
                     ATVVLSDVDEAAAEAAASELPGAAAFRCDVRDQNSVAELFERAGNEYGKVDIAVANAG
                     IATVSPLLEMSFDTWRTMMSINLDGVFLTVQHAARAMIATQTAGSIITMASVTAMAGV
                     PLVGHYAAAKAAVVSLTKTAALELRPTGIRVNAVLPGFAETELVTANRQRYEAGLGVD
                     FDGLIRQGQGGYVTVEDVAALALFLAGDRSRFCTGGAFVMDGGLTASLI"
     gene            complement(5836..>6327)
                     /locus_tag="VFI55_16145"
     CDS             complement(5836..>6327)
                     /locus_tag="VFI55_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7075596.1"
                     /translation="EIAKPITELTDDEFGMVLDINVLGTFRFTKAAVPALAAANGSAI
                     VNLSSVAGTAGSPLLTAYCASKGAVIRFTESAAVELRGAGIRVNAVCPGLIQTEMADR
                     LVAPIEAVMPITFDELVNLKQGRLGTPEEVAETVAFLASDEAGFVTGAHYLVDGGMTA
                     SLF"
BASE COUNT         1072 a         2074 c         2134 g         1047 t
ORIGIN      
        1 caaaataatc cgctaaccgt tcgcgtacac ccgctttcgt gaatccgggg gtcgatcgaa
       61 tcacgattgc gcgcacagca gagccggttc catattggct ggaatgccgt tccagcgaac
      121 gcgccgtggc actcatcaag atcgagttta tggggtatca actgcgacgg caggtggatc
      181 ttcgacccgc aagtcgctgc acaggcaaat ttgaattacc tgaaataagt ttgccgaaat
      241 ccattggcgg cgcaatctcg ctgaatcgcg aactgccggc gtatgcttct acagcgaccg
      301 gccgcccgga gtcgttatct gaaaaccgcc ttttcaacat ttgacaaata gcgtacctga
      361 ccggaacggc gctattcctg ttgaaccgat gccaaataag cgatttccat ttaggctgcg
      421 gccatgtcaa tcgaccgggc cgcgctcctc gccggcagca tccgattcgc ctcgggcgtc
      481 tccttcctcg tcgacccgct gcgcgcgaat cggctctggg gcgatccgga ggagccgacg
      541 ccgacggcgc gactgctgct gcggtcaatg ggctatcgcg atgcgttgat cggtggactg
      601 ctggtggcgg ccgcgcttcg cggcaaggac acccgaggct ggttcctagc ttccggcggc
      661 gccgacgcgg ccgacctgct cggcgggatg agcgtccatc gtgagttgaa gcccgcgcag
      721 cggctcgtcg ggctgggtgg tgcggtcgtc ggtgtcggcg tcgggctctg gggcgcgacg
      781 cgccggaccc cgagccccgc cggcccgctg acgggcgggc ggtcgtgagc atgcactcga
      841 ggaacgactg cgccgatcta cggttggccc cgggtggagg cagtttccac gagcgaccac
      901 tgactccgtt cacgctcggt cagctcgttg gacacgatcg caccgaaggc cgcggcgtcg
      961 cgtgcgaacc gggtcggcgc atcggccgcc gcgtccgggc cttcgagccg aaaacaactc
     1021 ggtgccagcg gaccgaacag gagggctcgc cgcaggtgcg gccagttgtc gaccgccggc
     1081 tccaccccgg cggcgcgggc aaagttcacg gccaccagat tcatccgggt cttctgtgac
     1141 gtccctcgct tcgagcgata agccttgatc gccaatcgct ggttgctttc cgtaggcgac
     1201 gagatggtgc cgccccacgt gtacgcgacc cacctggctt gaagttcaag cggcacaaag
     1261 tatccgcccg actggtccca catgcccata aaggccagcc ccggaaggtc gggatggaac
     1321 gtgtagtggt gggcgtccat atgcaccgcg tcgaggtcga ggatccgttg gatgtcttca
     1381 tccaggaagg gcagatgcag gttgaatccg gtaccgaaca cgatgccgtc aaattcctcg
     1441 gcgtgcccgt cgccgaaggc caccgtggag ccagcaatcg actccatcca gggacgcacc
     1501 gtgatccggc cctccgccac cagcggtagg tagtgctggt tgagggtgac cccggccacg
     1561 aagagggacg ggtccggcgt cggcgcgccg tactgctcgg ggctcccgct cgacgcgagg
     1621 acgatctcct tcagttgccg gtcgatctcg gccgccggca gcgtctcgtt cgccagcgtg
     1681 ccgtatcgcg tatagatgcg gtgatcggag gggactccgg cggcgaactt gggcaggacg
     1741 tacctctggc gccgctgggt cagcacgacg cgtgccgttc cgagttgggc gagttctgac
     1801 gcgatctcga gggcgctgat cgcgcagccc gccaccagaa cgcgcttgcc ttgataagcg
     1861 gaggcccctc ggtagtggta ggtagatttc gcttccgcgg acccggcgaa tgtctccagt
     1921 cccggcacat aggggatggc gggggagtgg aacctgcccg tcgccactac cactcgctcg
     1981 aagcgcgtgt cggtgccggc gtgacgcacg atccatccgg acccgtcgcg tctgtttcct
     2041 cggctgagga gttcgactcg tgtgccgaac tggatgcgcg aagcaaggcc gaaggtttcg
     2101 gcatagcggc gcaaatagtc gagaatctca cggttggacg ggtagacgag gtcggtgtcg
     2161 tgctcgaggt cgctgaaggt cgtgaggacg cgactgctat tcgtatgcat gctgggccac
     2221 acgccgcttc ggccgtccag gcccgcccac tgcccgccga gcatggtttc ccgttcgaag
     2281 atcgtcggct cgaacccctg cgacagcagc caacgcgccg cgatcagccc ggccgggccc
     2341 gccccgatca ccgcgacgct tgcagtcatg ctggaactct cgcacgggtc tgacttgatc
     2401 gccacgatct ggtgacaacc acgtagttgt atttggcgcg aatgtcaact ggtgcaagcg
     2461 catcggcgat gccgcagttg gtgaatcagg tcccgcagaa ggtttgatag gtgccgaagt
     2521 cctccggcgc gggactggcg taacgctcga gccccggtcg cttgtcgcac ggggcggtca
     2581 ccgcggcaag gagcgtcccg agcggatcga gatcgccggc cgtcgccgcg gtgagcgcct
     2641 cctcgaccag atggttgcgc ggaatgtaga tcgggttggt gcggtccatc aattcggcgt
     2701 ccggacccag ggcccgccag cgcgacatcc agtcgtcgaa gccggccagg tcgatgaaga
     2761 ggccgcgcgc gggttcggca tccgattccg cgccccgccg ggccgcctgg ccgaggtggc
     2821 ggaagaacga cgtgtagtcg acgttgctct gcctcagcag gggcagcaac tcgtccacca
     2881 acgtcgtgac gacatcggct ccgaggtgcg cggacaaacc cagcttggcg cgcatgccgg
     2941 acgaccacac cgactcgtac tcggggccga agaccccgaa cgactcctcg gcgatcgcga
     3001 tcgcgtcctg gacgtcgtcg gcaagcaacg gaagaaggga ttcggcgaac cgggcgaggt
     3061 tccagccggc gatggcgggc tggttgccgt aggcgtagcg cccccaggag tcgatcgaac
     3121 tgaagaccgt atcgggatcg taggcctcca tgaaggcgca gggtccgtag tcgatggttt
     3181 cgcccgagat cgtcatgttg tcggtattca tcaccccgtg gacgaagccg atcagcatcc
     3241 atcgggcgat gagcgatgcc tgggcggcga ccaccgcttc gaacagcgcg acgtagggcc
     3301 ggtctgcctc cgccgcgccc ggatggtgcc gggcgatagc gtggtcggcc aggcgccgca
     3361 gcaggtttat atcgccggtc gatgctgcgt actggaagct gccgacccgc aggtggctgc
     3421 tggcgacgcg ggcgagcacg gcgccgggca gtagcgtgtc gcgctggacc gagcggccgg
     3481 tagccactac cgccagcgag cgcgtcgtgg ggacgcccaa cgcgtgcatc gcctcactga
     3541 ccaggtattc gcgcagcatc ggtcccaccg cggccaggcc gtcaccaccg cgggcgaacg
     3601 gggtggggcc ggagcctttg aggtggatgt cacgcatgcg gccgtcgtcg tcgacgagct
     3661 cgcccagcag cagcgcccgc ccgtcgccca accgcgggac gaagccgccg aactgatgcc
     3721 cggcgtaggc ctgagccacc ggtgcggcac cctcggggac caggttgccc gccaggaaac
     3781 gcagcccatc ggaggtttgc agccatgcgc tatcgaggcc gaggtctgag gccagccgct
     3841 cgttgaggac gagcagccgc aagtcgggag ccgtgtccgc ctgccagcgc acggccatct
     3901 ccggcaactc gcgcaagaac cggtgttgta acgcgacggt cgtgtccggt gtgacgctca
     3961 cgtcaccgag actacggaaa gtacggtccg ccgaagtgga tgcggggcat cccgatctcc
     4021 ccgctccggg catgccgcta cggtttaggg aacacgcgcc aacacccacg ggatgaagat
     4081 gacgacagat cacgcaatgt atgaacacgt ggacggtccc gcggcggatc ccgccgacat
     4141 cggctcacgc atcgatcctg ttctcgcccg cagttggctc ctggtcaatg gcgcccatgc
     4201 cgatcgattc cagtcgtcgg cgcactcgcg cgccgacatc gtggtgctcg acatcgagga
     4261 cgcggtcgcg cccaaagaca agcaagccgc ccgcgacaac gtggtgcggt ggctcaccgc
     4321 cgacaacacg gactgggtcc gggtcaacgg cttcggcacc ccgtggtggg cggacgattt
     4381 ggcggcgctg gccggcaccc cgatcggcgg cgtgatgctg gcgatggtcg aatcggtgga
     4441 ccacgtcacc gagaccgcgc agcggctgcc caacgtgccc atcgtggcgc tggtcgaaac
     4501 ggcccggggc ttagagcgca tcaccgaaat cgccgcggcc aaggggacct tccggctggc
     4561 cttcggcatc ggcgatttcc gccgtgacac cggattcgga gaagagccga ccaccctggc
     4621 ttacgcgcgg tcgcggttca cgatcgcggc caaggcggcc aacctgccca gtgcgatcga
     4681 cgggccgacg atcggctcca acgcgttgaa gctcatcgag gcgacggcgg tttccgtcga
     4741 attcgggatg accggcaaga tctgcctcac gccggaccaa tgcgccgtgg tgaacgaggg
     4801 actgtccccg tcacaggacg aaatcgcttg ggcaaaggag tttttcgccg agttcgaacg
     4861 cgacggggga gagatccgca acggctccga cctgccgcgc atcgcccggg ccaccaagat
     4921 cctcaagctg gcccgcgcct atggcatcga ggtgtccgag ttcgaggacg aacgggttca
     4981 ctcgcccgct ccgacggaca cctaccacta ctgagcgaca gtcagatgag gctcgcggtg
     5041 agcccgccat ccatcacgaa ggcgccgccg gtgcagaacc tcgaccggtc gcccgcgagg
     5101 aagagcgcca gggcggccac gtcttccacg gtgacataac ctccctggcc ctgtcggatc
     5161 aggccatcga agtcgacgcc caatccagcc tcgtaccgct ggcggttggc cgtgacgagt
     5221 tcggtctcgg caaagcccgg cagaaccgcg ttcactctga ttccggtcgg ccgcagttcg
     5281 agcgccgccg tcttggtcaa gctcacgacg gcggccttcg ccgccgcata gtgcccgacc
     5341 agcggaacgc ccgccatcgc cgtcaccgat gccatcgtga tgatggatcc cgcggtctgg
     5401 gtggcgatca tcgcccgcgc cgcgtgctgc acggtcagga aaacgccgtc caggttgatc
     5461 gacatcatgg tgcgccaggt gtcgaaggac atctccagca gcggtgaaac cgtggcgatt
     5521 ccggcgttgg cgacggcgat gtcgaccttg ccgtactcgt tgccggcccg ctcgaagagt
     5581 tcggcgacgc tgttctggtc acgtacgtcg cagcggaacg ccgccgcccc cggcagctcg
     5641 ctggcggccg cctcggcggc tgcctcgtcg acgtcggaca gcaccacggt cgcgccgtgg
     5701 gcggcgtaga cccggccgat ctggcgaccg ataccgcgag ccgcgccggt gatcacggcg
     5761 accttcccgg ccagcgagcc ggaagcggcc tcggccgcgc tgtggctgga ttgcgcattg
     5821 gtcatggttg ctctcctaga aaaggctggc ggtcatgccg ccgtcgacga ggtagtgggc
     5881 gccggtgacg aaacccgctt cgtccgaggc gaggaaggcg acggtctcgg ccacctcctc
     5941 gggggtgccg aggcggccct gcttgaggtt gacgagctcg tcgaaggtga tcggcatgac
     6001 cgcttcgatc ggcgcgacca gccggtcggc catctcggtc tggatcaagc ccgggcacac
     6061 cgcgttgacg cgaatcccgg ccccccgaag ctcgaccgcc gcggattcgg tgaagcggat
     6121 gacggcgcct ttcgatgcgc agtacgcggt gagtaacggg ctgcccgcgg tgccggcgac
     6181 ggaggacaga ttcacgatcg cgctgccgtt ggccgctgcc agggcgggca ctgctgcctt
     6241 ggtgaaccgg aacgtcccca ggacgttgat gtcgagcacc attccgaatt cgtcgtcggt
     6301 gagttcggtg attggcttgg cgatttc
//
LOCUS       DASSGQ010000363         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_118513,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000363 DASSGQ010000000
VERSION     DASSGQ010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118513"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VFI55_16150"
     CDS             <1..404
                     /locus_tag="VFI55_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET7075597.1"
                     /translation="WRRRLDVSAMARSAECLLGEHDFAAFCRPRMGSSTIRTLQSLDV
                     RRESCGEVSCTAQADAFCHSMVRSLVGALLAVGDGRRPPEWVSTLLARTSRAHEIAVA
                     AAQGLTLVAVGYPRPDRYAAQAAAARRRRGS"
     gene            complement(409..999)
                     /locus_tag="VFI55_16155"
     CDS             complement(409..999)
                     /locus_tag="VFI55_16155"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7075598.1"
                     /translation="MPRRATRTAPTVILHGLHGNEPEHWQTWLAAQLQDTGREVRYPE
                     LPDVNQPERLAWLEVLRTTLDGLTDGEFDVVCHSLGCLLWLHHVAGGTSAPRPARVAL
                     VAPPSPRSTVPELADFLPVPLVIDELRRAADGTVLACGTDDPYCPEGAQVAYGLPLRL
                     PTTVVPGGGHLNVAAGYGPWPAMLDWCGRDKLAFLV"
     gene            1663..>2098
                     /locus_tag="VFI55_16160"
     CDS             1663..>2098
                     /locus_tag="VFI55_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075599.1"
                     /translation="MQAATQAVADAWVATRPTVNGKCVTVTVDQENPSAAVQSLGVLP
                     ANTVWIPDSRFWADQLRAASPTLRSAVTISGSVGSSPLVVATAPARATSLAAAAKAGW
                     AGVLMGSAVVAVPDPLSTTEGALSALAVQSAVGSPANSSAMLV"
BASE COUNT          337 a          693 c          707 g          361 t
ORIGIN      
        1 gttggcgccg tcggttggat gtcagcgcga tggcccgcag cgcggagtgc ttgctcggcg
       61 agcacgactt cgccgcgttc tgccgtccgc ggatgggcag ttcgacgatt cggacactgc
      121 aatcgctcga cgtccgccgt gagtcctgtg gggaggtgag ctgcactgcc caagccgacg
      181 cgttttgcca ttccatggtg cgatcgttgg tcggggcgtt gcttgcggtc ggtgatggcc
      241 gtcgcccacc agagtgggtg agcaccctgc tggcccgcac ctcccgggcc cacgagatcg
      301 cggttgctgc cgcccagggg ctgacgctcg tggccgtcgg gtatccgcgg ccagatcggt
      361 acgccgctca agccgccgcc gcccgcagac gacgaggcag ctagtggctc acaccaggaa
      421 cgccagcttg tctcggccgc accagtccag catggccggc catggtccat atccggccgc
      481 cacgttcaga tggccaccgc cgggtaccac tgttgtcggc agccgcaacg gaagtccata
      541 ggcgacctgt gcaccctcgg ggcagtaggg atcatccgtt ccgcaggcca gcacagttcc
      601 gtcggccgcc cggcgcagtt cgtcaataac caacgggacg ggcagaaagt ccgccagctc
      661 cggaaccgtc gagcgcgggg acggtggggc gacaagagcc acgcgcgcgg gtcgcggcgc
      721 actcgtaccg ccggcgacgt ggtgcagcca cagcaggcag ccgagcgaat gacagacgac
      781 gtcgaattct ccatcggtca agccatcgag ggtggtgcgg aggacctcga gccacgccag
      841 ccgctcgggt tggttcacat cgggtagttc gggatatcga acctcgcggc cggtgtcctg
      901 caactgcgcg gccagccagg tttgccagtg ttcgggttcg ttgccgtgca gtccgtgcag
      961 gatcaccgtg ggcgctgtcc gggtagcacg tctgggcacg tccggcagcg tagatcacgc
     1021 gtccgctgat gatcttgacg gttgggcttc tcagcagtcc caccagcacc aaccgtgcgc
     1081 ccggacgcga cgcccggaat cgcgaggcac ccgaaaagtg ccgaccgcgg aggatcacca
     1141 ttcggatacg gaccgcgaaa tcgcggctgt cagtaactgg cgagtgacaa atccaccggt
     1201 tgaagttcgg gtaggtcgct cggcgtacag ctaggccaca acgcaggcga tcggtattcg
     1261 cacggtgatc gtgctggtac cactggttac attcgccgac ccgaacgccc gatgccgcgt
     1321 cgtaggtgtc tcgtacttcc tgaagatgat gagtagtatc ccgcggatat ccacggtgat
     1381 cgggcaactc cgcccgtccg ggtctagctg gccttggagg cgccacttgc gatgactggc
     1441 aggcacagtg ctcggatcgc aagccgtgcc cgtcttcggg ctccttcatc acacagaggt
     1501 cggcgcgtcg ccttgatagc cgccgtggct gtcgtgatcg ccgcggcagc ggtgaccatc
     1561 acggtcttgc gaacgggcca accgtccgga cgacctgccg ccgcgacaag ctctcacccc
     1621 tgcttgaacg gagtgccgct cagcgtcatt actgcgccgc aggtgcaagc ggcgacccag
     1681 gccgtcgccg acgcgtgggt tgcgaccagg ccgaccgtga acggaaagtg cgtcacggtc
     1741 acggtcgatc aggaaaaccc aagtgcggca gtccaatcgc tgggcgtatt gccagccaac
     1801 acggtctgga ttcccgactc tcgcttctgg gcggaccagt tgcgtgccgc cagcccgacg
     1861 ctccgctcgg cggtcacgat cagcggctcg gtcggcagct cgccgctcgt ggtggcaacg
     1921 gcgccggccc gcgcgacgtc gctcgccgct gcggcgaagg cgggatgggc cggcgttctg
     1981 atggggtcgg ccgttgttgc cgtacccgat ccgctgagca caaccgaggg cgcattgtct
     2041 gctcttgctg tgcaatcggc cgtcgggtcg cccgcgaact cttcggccat gctggtcg
//
LOCUS       DASSGQ010000364         2311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_604634,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000364 DASSGQ010000000
VERSION     DASSGQ010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604634"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VFI55_16165"
     CDS             <1..659
                     /locus_tag="VFI55_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7075600.1"
                     /translation="AFDEAAVIAEVIADVRLVFDNVVCVDDGSTDDTGEIARRSGAHL
                     VRHPINLGQGAAIQTGVEYARKQPGAQVFATFDADGQHRVKDLIAMIDRLCIGDVDVV
                     IGTRFGRQQGSRPPFLKRVVLQSAARLSRRGRRLGLTDTNNGLRVFNRTVADGLNITM
                     SGMSHANEFVMLIAENRWRVVEQPVEVLYTEYSKSKGQPLLNGVNIIFDGFLRGRIPR
                     "
     gene            673..1014
                     /locus_tag="VFI55_16170"
     CDS             673..1014
                     /locus_tag="VFI55_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HET7075601.1"
                     /translation="MNWIQVLLIGSIIALLVYLLRSRRNARSRAWVKVGYIAFVLAGV
                     YAVLRPGDTTVVAHWFGIGRGSDLMLYALIMAFSFTTLSTYMRFKDLELRYARLARAV
                     ALEGAQAPEYS"
     gene            complement(1011..1955)
                     /locus_tag="VFI55_16175"
     CDS             complement(1011..1955)
                     /locus_tag="VFI55_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7075602.1"
                     /translation="MRALVTGAAGFIGSTLVDRLLADGHTVVGLDNFATGRTSNIEYL
                     AGNPAYVFVQADIVTADLLAILGEHQPEVVFHLAAQIDVRRSVADPQFDASVNVIGTV
                     RLAEAARSTGVRKVVHTSSGGSIYGTPSQFPTPETVPTDPASPYAAGKMTGEIYLNTF
                     RHLYGLDCSHIAPANVYGPRQDPHGEAGVVAIFAQALLLGKPTKVFGDGTNTRDYVYV
                     DDVVNAFVKASGPVGGGQRFNIGTGIETSDRQLHSAVAAAVGGPDDPEFAPARLGDLR
                     RSSLDNGLAERVLGWHPQVQLDEGVRRTVEYFRKLQTV"
     gene            1978..>2311
                     /locus_tag="VFI55_16180"
     CDS             1978..>2311
                     /locus_tag="VFI55_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075603.1"
                     /translation="MRATRLPPVAVAAAALITVQLGIRAVLAFGGYFYWDDLILIGKA
                     GTQGMLSPSYLFDDHDGHVMPAAFLVAGAIIRLAPLDWTGPAISLLVLQLLASLALLR
                     ALCVILGRR"
BASE COUNT          387 a          737 c          771 g          416 t
ORIGIN      
        1 ccgccttcga cgaagccgcc gtcatcgccg aggtgatcgc cgatgtgcgc ttggtcttcg
       61 acaacgtcgt ctgcgtcgac gacgggagca ccgacgacac cggcgagatc gccaggcgat
      121 ccggcgcaca tctggtgcgc catcccatca accttggcca gggtgcggcc atccagaccg
      181 gcgtcgagta cgcccgcaaa cagcctggcg cgcaggtctt tgccacgttc gacgccgacg
      241 gtcagcatcg cgtcaaagac ctgatcgcga tgatcgaccg gctctgcatc ggtgacgtcg
      301 acgtcgtcat cgggacccga ttcggcaggc aacagggcag ccgaccgccg tttctgaaac
      361 gcgttgtcct gcaatccgct gcacggctga gccggcgcgg tcggcggctt ggcttaaccg
      421 acaccaacaa tgggctgcgg gtgttcaaca ggacggtcgc cgacggcctg aacatcacca
      481 tgagtggcat gagtcatgcc aatgagttcg tgatgttgat cgccgaaaat cgttggcggg
      541 tggttgaaca gccggtcgag gtgctgtaca cggagtattc gaaatcgaag ggccagcccc
      601 tgctcaacgg cgtgaacatc atcttcgacg ggttcctgcg agggaggatt cccagatgaa
      661 agggactgaa ggatgaattg gatccaggtg ctgctgatcg ggtcgatcat cgcattgctg
      721 gtctatctgc tgcggtcccg caggaatgcg cggtcccggg cctgggtcaa ggtcggatat
      781 atcgcgttcg tattggccgg cgtctatgcc gtgctccggc ccggcgacac cacggtggtc
      841 gcacactggt tcggcatagg ccgcggcagc gacctgatgc tctacgcgtt gatcatggcg
      901 ttcagcttca cgacgctgag cacctacatg cggttcaaag acttggagtt gcgctacgcg
      961 cgactggccc gcgctgtggc gttggagggc gcacaggcgc ccgagtacag ctagaccgtc
     1021 tgcagctttc ggaaatactc gaccgtgcgg cggacaccct cgtcgagttg aacttgcggg
     1081 tgccagccca aaacccgttc ggccaagccg ttgtccagag acgaccgccg cagatcgccg
     1141 aggcgcgcag gagcgaactc cgggtcgtcg ggccccccga cggccgcggc caccgccgaa
     1201 tgcagttggc ggtccgaggt ttctattccg gtgccgatgt tgaaacgctg cccaccgccc
     1261 accggcccgg atgccttgac gaaggcgttc acgacgtcgt cgacgtacac gtagtcgcgg
     1321 gtgttggtgc cgtcgccgaa caccttggtg ggcttaccca agagcagcgc ttgcgcgaag
     1381 atcgccacca cgcccgcctc gccgtgcggg tcctggcggg ggccgtagac gttggcgggc
     1441 gcgatgtgtg agcaatccaa cccatacagg tgtctaaagg tgttcaggta gatctcgcct
     1501 gtcatcttgc ccgccgcgta tggcgatgcc gggtcggtgg gaaccgtctc gggggtcggg
     1561 aactgcgacg gggtgccgta gatggatccc cccgacgagg tatgcacgac tttgcggaca
     1621 ccggtggacc gtgcggcttc ggccaggcgc accgtgccga tgacgttgac cgaagcgtcg
     1681 aattggggat cggccaccga gcgccgcacg tcgatttgcg ccgccaggtg gaacaccacc
     1741 tccggctggt gctcgcccag gatggcgagc agatcggcgg tcacgatgtc ggcctgcacg
     1801 aaaacgtacg cggggttgcc ggccagatac tcgatgttgg acgttcgccc ggtcgcgaaa
     1861 ttgtccagtc ccacgaccgt gtgaccatcg gccagcagac ggtcgactag cgtcgacccg
     1921 atgaaaccgg ctgccccggt aaccagtgcg cgcaccagga caccataccg gcgggcggtg
     1981 cgggcgaccc gcctacctcc ggttgccgtc gccgccgccg cgctgatcac ggtgcagctg
     2041 gggatacgcg cggtgctggc gttcggcggt tacttctact gggacgacct cattctcatc
     2101 gggaaggccg gcacccaggg catgttatcg ccgtcgtacc tgttcgacga ccatgacggc
     2161 cacgtgatgc cggccgcctt cctggtcgcc ggcgcgatca tccggctggc gccgctggac
     2221 tggacggggc cggcgatcag cctgctggtg ctgcagttgc tggcctcgct ggccctgctg
     2281 cgcgcgctgt gcgtcatcct cggccggcgc t
//
LOCUS       DASSGQ010000365         6879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1152131,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000365 DASSGQ010000000
VERSION     DASSGQ010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152131"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VFI55_16185"
     CDS             complement(<1..632)
                     /locus_tag="VFI55_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7075604.1"
                     /translation="MTTNRGRRSDLSCQRGISRQTFLRGAAGMLASGAVFGTARAAAD
                     PAAGWGGLASSIGGSVVLPANGAQFATGKQVFNSFYNNSNPAAVVTVSSQSDVQRAVA
                     FATANGLKVAPRGGGHSYIGASTANGTMVLDLRGLPGGPNVDGGSVTVAPATDLYAVH
                     QALAGAGRAIATGSCPTVGIAGLALGGGIGPDCRHAGLTCDALQSATVVLP"
     gene            912..1322
                     /locus_tag="VFI55_16190"
                     /pseudo
     CDS             912..1322
                     /locus_tag="VFI55_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167979.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
     gene            complement(1325..1630)
                     /locus_tag="VFI55_16195"
     CDS             complement(1325..1630)
                     /locus_tag="VFI55_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3017 domain-containing protein"
                     /protein_id="HET7075605.1"
                     /translation="MSARAIQRGSIGTALRAQWPIVVVGMIFVTAFVWVGAKFWRRGA
                     LLIGIGAGVAAVLRLVLSDERAGLLVVRGKGIDFATTATVAAAMVYIAWTIDPLGTG"
     gene            complement(1627..2472)
                     /locus_tag="VFI55_16200"
     CDS             complement(1627..2472)
                     /locus_tag="VFI55_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HET7075606.1"
                     /translation="MGAITLDGKATRDEIFVDLKERVAALTASGRIPGLGTILVGDDP
                     GSQAYVRGKHADCAKVGITSIRRDLPADISAAQLDETIDELNANPECTGYIVQLPLPR
                     HLNENAALERVDPDKDADGLHPTNLGRLVLMEPAPLPCTARGIVHLLRRYDVEIAGAH
                     VVIIGRGVTVGRPLGLLLTRRSENATATLCHTGTRDLPALTRQADIIVAAVGVPHLLT
                     ADMVRPGAAVVDVGVSRTESGLVGDVHPDVWEVAGHVSPNPGGVGPLTRAFLLTNVVE
                     LAEGR"
     gene            2513..3718
                     /locus_tag="VFI55_16205"
     CDS             2513..3718
                     /locus_tag="VFI55_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HET7075607.1"
                     /translation="MSDVPDVFSPARLGPLTLRNRVIKAATFEARTPDALVTDDLIEY
                     HRRPAAGGVGMTTVAYCAVSPGGRTGGNGIWMRPEAVPGLRRLTEAIHAEGAAVSAQI
                     GHAGPVADARSNKATALAPVRFFNPIGMRFAKKATRDDIDNVVAAHANAARLAIDAGL
                     DAVEIHLGHNYLASAFLSPLLNRRDDEFGGSLENRARVARGMVMAVRRAVDKEGAKIA
                     VTAKLNMADGVRGGITIEESLTTATWLQDDGGLDAIELTAGSSLVNPMYLFRGDAPVK
                     EFAGAFKPPLRWGMRMTGKKFLREYPYRDAYLLRDAKLFRAELTMPLILLGGITNRET
                     MDLAMTEGFQFVAMARALLAEPDLINRIVADGARHSVHSACTHCNRCMPTIYSRTHCV
                     VTGAPDATG"
     gene            3748..6471
                     /locus_tag="VFI55_16210"
     CDS             3748..6471
                     /locus_tag="VFI55_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7075608.1"
                     /translation="MTPPAPPTLTVRHDGSERTFAAGHDVVVGRDLRADMRITHPLIS
                     RAHLLLRFDQGRWLAIDNGSLNGTFANGRRVPVVDIHDGQSVNIGNPDGPQLTFEVGR
                     HQGMAGRPPRTESMGIPVAAPASAAPWPASQTSGQPGPPVAAPPAPPPATPGRGPQWA
                     SPAGRRPHVGPHPPPGQPVYPTSGGGRPPAHPTGAPPPPPNFQLHPPMAAAGAPQAAP
                     HTHMSPSVAKPPEVGSLATKMFQALLPSRSGALPKPTESVTIGRATDNDIVIQDVLAS
                     RHHAFLTQTPLGTEIRDAHSVNGTFVNGVRVGSALLTEGDVVTIGNVDLVFARDTLVR
                     RTEAATRTGGLEVNSVCYTVDHGKQLLDHITLTARPGTLTAIIGGSGAGKTTLSRLIA
                     GYTSPSSGTVTFEGHNIHAEYASMRSRIGMVPQDDVVHRQLTVNQALGYAAELRLPPD
                     TSKADREQVVAQVLEELELTKHADTRVDKLSGGQRKRASVALELLTGPSLLILDEPTT
                     GLDPALDRQVMMMLRQLADAGRVVLIVTHSVSYLDVCDQLLLVAPGGKTAFLGPPSQI
                     GAAMGTTNWADIFAQVGADPDEANRRFLAENQPPPAPASDVSPGDLGEPVHTDVFHQF
                     STIARRQVRLVVSDRGYTVFLALLPFLIGILTLTVRGNSGYGMSDPLGNNPAQPDQIL
                     VMLNVGAVFMGTALTIRDLIGERPIFRREQAVGLSTGAYLGAKIVVFSAFAIVQAAIA
                     TVISVVGWGRPLSGAVVLGNVDVELFVTVAATCVASALLGLALSALARSQDQIMPMLV
                     VAIMSQLVFSGGMIWVTDRAVLDQLSWATPARWGYAASASTIDTHRLVPGPTDPKDQH
                     WNHTASAWLFDMGMLGVLCVAYTAIVWWRIRLKRHTPSRGHLLQRNRPKKE"
     gene            6468..>6879
                     /locus_tag="VFI55_16215"
     CDS             6468..>6879
                     /locus_tag="VFI55_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET7075609.1"
                     /translation="MNQPAQPVLTVRLGQSEGNFAAGRDVVIGSDVRADLRVAHPLIA
                     RAHLLLRFGNGKWIAVDNNSLNGVFLNGQRVPTVDIQDGQSINIGKPDGPRVTFQVGH
                     HRGNVGLLPTMATIPTPGGPPPAPPQPPIPPGPRG"
BASE COUNT         1144 a         2477 c         2237 g         1021 t
ORIGIN      
        1 ggcaacacca cggtggccga ctgcagcgca tcacaagtca ggcccgcatg ccggcaatcg
       61 ggcccgatcc caccgcccag cgccagcccc gcgatgccga cggttgggca actgccggtg
      121 gcaatcgcgc gcccggcgcc ggccaatgcc tggtgaaccg cgtacagatc ggtcgccggc
      181 gcgaccgtga cgctgccgcc gtcgacgttc ggcccgccgg ggagcccgcg caggtccagc
      241 accattgtgc cgttggcggt cgacgcgccg atgtacgaat gccccccgcc gcgcggggcg
      301 accttgagcc cgttggccgt tgcgaacgcg acggccctct gcacgtccga ttgcgacgag
      361 accgttacca ccgcagcggg attggagttg ttatagaacg aattgaaaac ttgtttgccc
      421 gtggcgaatt gagcgccatt ggcgggcaac accacgctgc ctccgatcga ggaggccaaa
      481 ccgccccagc cggcggcagg gtccgcggcg gcccgcgcgg tgccgaaaac cgctccgctc
      541 gctagcattc cggcggcgcc gcgaagaaac gtctggcgcg agatgccccg ctggcaggaa
      601 aggtccgacc gacggccgcg attcgtcgtc atggcccgga ttcttaacca tccagcaggc
      661 gaaaatccgg cctctcagca cgtgtcagat cacagtgtgg cgtcgatccg tcggtggaag
      721 gagcagaccc ggtcggccgc tcctgcacgg gctttcgccc attaaccgat gctgtgcgtg
      781 cgcggccctt acaaaacgct tattgggcaa agctttatcg cactgtgttg taaatgtgac
      841 agttatagac cagagttcct aacgtgattt gagtgtccca tgtgcactca gatgcggatg
      901 gggaggctgg catgaaggca ccggtaatag ctcaacgatg gatccggttg tatcgccatc
      961 agccgctgac cattcgcctg ctggcgctgg ccgcagccct ggtcaccgcg gcggcggcat
     1021 tcacggcgcc ggccgaggcg gaccccgccg acgacgactt catcgacgcg ctgaaccacg
     1081 ccggcgtcga gttcggcgaa ccgggaaacg cgatggccgt aggccaggcg atctgcccga
     1141 tgctcgccca gccaggcgga aacttcgccg ccgtcgcggc cagcgtcagc caccggggca
     1201 tgtcaccgca gatggcgcgg atgttcacca ccatcgcgat tcaggtgtac tgcccgcagg
     1261 agatcgcaaa cctggcgggc ggcaacgtgc atggcctgcc gcaagtcccg gggatgccag
     1321 ggatttagcc ggtgcccagc gggtcgatgg tccacgcgat atagaccatc gccgccgcga
     1381 ccgtcgctgt cgtcgcgaag tcgatgccct taccgcggac gaccaacaat ccggcacgct
     1441 cgtcggacaa caccaagcgc aggaccgccg ccaccccggc tccgatgccg atcagcaggg
     1501 cgcctcgacg ccagaacttg gcgcccaccc acacgaaagc cgtcacgaag atcatcccca
     1561 ccaccacgat cggccactgc gcccgaagcg cggtcccgat agacccccgc tgaatggccc
     1621 gcgcgctcat cgcccctcgg ccagctcaac gacgttggtc aacaaaaacg cccgggtcaa
     1681 cgggccgacg ccaccgggat tcggcgacac gtggccggcc acctcccaca cgtcgggatg
     1741 cacgtcgccg acgagtccgc tctcggtgcg gctgacgccg acgtcgacga ccgcggcgcc
     1801 gggacgcacc atgtcggcgg tcagcaggtg cggcacaccg acggcggcca cgatgatgtc
     1861 cgcctgccgg gtcaacgcgg gcaggtcgcg agttccggtg tggcacaacg tcgcggtcgc
     1921 gttctcggag cgacgggtca gcagcagccc caacgggcgc ccgaccgtca caccacgccc
     1981 gatgatcacc acgtgcgccc cggcgatctc gacgtcgtag cgacgcagca ggtgcacgat
     2041 gccgcgcgcg gtgcacggca gcggcgccgg ttccatcagc acgagccggc cgaggttggt
     2101 cgggtgcagc ccgtcggcgt ccttgtccgg gtcgacgcgc tccagagctg cgttctcgtt
     2161 gagatgcctg ggcagcggca actggacgat gtagccggtg cattcggggt tggcgttgag
     2221 ttcgtcgatg gtctcgtcga gctgagcggc gctgatgtcg gcgggcaggt cacgacgaat
     2281 cgacgtgatg ccgaccttcg cgcagtcggc gtgcttgccc cgcacgtagg cctgcgagcc
     2341 ggggtcgtcg ccgaccagga tggtgcccag tccgggtatg cgacccgatg cggtcagtgc
     2401 cgccacccgt tccttcaggt cgacgaagat ctcgtcgcgg gtggccttgc cgtccagcgt
     2461 gattgcgccc acgcctgaca gtctggcacg cccgccgcgg tacgctgccc gcatgtccga
     2521 cgtcccggac gtattcagtc cggccaggct gggtccgctg acgctgcgca accgtgtcat
     2581 caaagccgcg acattcgaag cgcgcacgcc cgacgcgctg gtgaccgacg acctcatcga
     2641 gtaccaccgg cggccggcgg ccggcggggt cggcatgacg accgtcgcct actgcgccgt
     2701 ctcccccggc gggcgcaccg gcggcaacgg gatctggatg cgacccgaag cggtgcccgg
     2761 attgcggcgg ctgaccgagg cgatccacgc cgagggcgcc gcggtcagcg cacagatcgg
     2821 ccacgccggc ccggtggccg acgcccgctc caacaaggcc accgccctgg cgccggtgcg
     2881 cttcttcaac ccgatcggga tgcggttcgc caagaaggcg acccgcgacg acatcgacaa
     2941 cgtggtggcc gcgcacgcaa acgccgcgcg gctcgccatc gacgccggcc tcgatgcggt
     3001 cgaaatccat ttaggccaca actatctggc gagcgcgttt ctgtcaccgc tgctcaaccg
     3061 tcgcgacgac gaattcggcg gatcactgga aaaccgcgcc agggtggccc gcggaatggt
     3121 gatggccgtt cgtcgcgccg tggacaaaga gggagcgaag atcgcggtca ccgccaagct
     3181 gaacatggcc gacggtgtcc gcggcggcat caccatcgaa gagtcgctga ctaccgccac
     3241 ctggctgcag gacgacggcg ggctggacgc gatcgagctc accgccggca gctcgctggt
     3301 caacccgatg tacctgttcc gcggcgacgc ccccgtcaag gagttcgcgg gcgctttcaa
     3361 gccgccgctg cgctggggca tgcgcatgac aggcaagaag ttcctccgcg agtaccccta
     3421 ccgcgacgcg tacctcttgc gcgacgccaa gctgttccgc gccgagctga cgatgccgct
     3481 gattctgctc ggcggtatca ccaaccgcga aacgatggac ctcgcgatga ccgagggttt
     3541 ccagttcgtc gcgatggccc gggcgctgct ggccgagccc gacctgatca atcggatcgt
     3601 cgccgacggc gcccggcaca gcgtccattc ggcgtgtacg cactgcaatc ggtgcatgcc
     3661 gacgatctac agccggaccc actgcgtcgt gacaggggcc cccgacgcta ccgggtgaaa
     3721 gccctcccac cggctagagt tgtggtaatg actccaccag ccccgccgac gttgaccgtc
     3781 cgccatgacg gctccgaacg caccttcgcg gcgggtcacg acgtggtggt ggggcgcgat
     3841 ctgcgcgccg acatgcgcat cacgcaccca ctgatttcgc gcgcccacct attgctgcgc
     3901 ttcgaccagg gcaggtggct ggcgatcgac aacggctcgc tcaacggcac cttcgccaac
     3961 ggccgccgcg tgccggtggt cgacatccac gacggccaga gcgtcaacat cggaaacccc
     4021 gacggtccac agctgacctt cgaggtcggg cgccatcaag ggatggccgg acgcccgccc
     4081 cgcaccgagt cgatgggcat acccgtggcg gctccggcgt cggcggcacc gtggccggcc
     4141 tcgcagacgt cgggtcagcc gggtcctccc gtcgcggctc cgcccgcgcc cccgcccgcc
     4201 actccaggcc gagggccgca atgggcctcg ccggcggggc ggcgaccgca cgtcggtccg
     4261 cacccgccgc cgggccaacc cgtgtatccg accagcggtg gggggcggcc acccgcgcat
     4321 ccgaccggcg cgccgccacc gccacccaac ttccagctgc acccgccgat ggccgccgcg
     4381 ggggcgccgc aggcggcacc gcacacccac atgtcgccca gcgttgccaa gccacccgag
     4441 gtgggcagcc tggcgaccaa aatgttccag gcgttattgc cgtcccggtc cggtgcgctg
     4501 ccgaagccga cggagtcggt caccatcggc cgggccaccg acaacgacat cgtcattcag
     4561 gacgtcctgg cctcgcgcca tcacgcattc ttgacccaga cgccgctggg caccgagatc
     4621 cgcgacgcgc acagcgtcaa cgggaccttc gtcaacggtg tccgggtcgg gtcggcgctg
     4681 ctgaccgagg gcgacgtggt caccatcggc aacgtcgacc tggtgttcgc ccgcgacacg
     4741 ttggtccgcc gcaccgaggc cgcgacgcgc accggcggcc tggaagtgaa ctccgtctgc
     4801 tacacggtcg accacggaaa gcaactgctc gaccacatca cgctgaccgc ccgtcccggg
     4861 acgctgaccg ccatcatcgg cgggtcgggc gccggcaaga ccacgctgtc gcggctgatc
     4921 gccggataca ccagccccag ctcgggcacg gtgacgttcg agggccacaa catccacgcc
     4981 gagtacgcgt ccatgcgcag ccgcatcggg atggtcccgc aggacgacgt cgtgcaccgc
     5041 cagctgaccg ttaaccaggc cctgggctat gccgccgaac tgcgcctgcc gcccgacacc
     5101 agcaaggccg accgcgaaca ggtcgtcgcg caggtcctcg aggagctcga gttgaccaag
     5161 cacgccgaca cccgcgtcga caagctgtcc ggcggccagc gcaaacgcgc ctcggtggca
     5221 ttagagctgc tcaccggacc gtcgctgtta atccttgacg agccgaccac cggcctggat
     5281 cccgcgctgg atcgccaggt gatgatgatg ctgcggcagc tggccgacgc cggccgtgtg
     5341 gtcctgatcg tcacccactc ggtgtcctac ctcgatgtct gcgaccagct gttgctggtc
     5401 gcccccggcg gcaagacggc attcttgggc ccgcccagcc agatcggcgc ggcgatggga
     5461 accaccaact gggcggacat cttcgcccag gtgggcgccg atcccgatga ggccaaccgt
     5521 cgcttcctgg ccgaaaacca accgccgccg gcacctgcgt ccgacgtcag cccgggcgac
     5581 ttgggcgaac cggtgcacac cgacgtgttc caccagttct ccacgatcgc gcgccgccag
     5641 gtgcggctgg tggtctccga ccgcggctac acagtcttcc tggcgctgct gccgttcctc
     5701 attggaatcc tcaccctcac cgtgcgcggc aacagcggat acggaatgtc agacccgctg
     5761 ggcaataatc ccgcgcagcc cgaccagatt ctggtcatgc tgaatgtcgg cgcggtattc
     5821 atgggcaccg cgctgaccat tcgcgacctc atcggcgagc gtcccatctt ccgccgcgaa
     5881 caggcggtcg gtctgtcgac cggggcatat ctgggcgcca agatcgtggt gttcagcgcg
     5941 ttcgcgatcg tgcaggccgc aatcgcaacg gtgatctcgg tggtcggctg gggcagaccg
     6001 ctgtcgggcg ccgtggtgct gggcaacgtc gacgtcgaac tgttcgtcac cgtggccgcg
     6061 acctgtgtag cctccgcact cctcggactg gccctgtcgg cactcgcccg ctcgcaagac
     6121 cagatcatgc cgatgctggt ggtcgccatc atgtctcagc tggtgttctc cggcggcatg
     6181 atctgggtca ccgaccgcgc cgtgctcgat cagttgtcgt gggccacgcc cgcccgatgg
     6241 ggttacgccg cgtcggcgtc gacgatcgac acccaccgcc tggtaccggg cccgaccgac
     6301 cccaaagacc agcactggaa ccacaccgcg agcgcgtggc tgttcgacat ggggatgctc
     6361 ggggtgttgt gcgtcgccta taccgccatc gtgtggtgga ggatccggct gaagcggcac
     6421 acgcccagcc gcggccacct tctgcagagg aatcggccga agaaggaatg aatcagcccg
     6481 cgcagcccgt cctcacggtt cggttgggcc aatcggaagg taacttcgcg gccgggcgcg
     6541 acgtggtgat cggcagcgat gtgcgtgccg acctgcgcgt ggcgcacccg ctcatcgcac
     6601 gcgcgcacct gctgctgcgg ttcggcaacg gcaagtggat cgcggtcgac aacaattcgc
     6661 tgaacggggt cttcctcaac ggccagcggg tgccgacggt cgacatccag gacggccaga
     6721 gcatcaacat cggcaagccc gacggacctc gcgtcacctt ccaagtcgga caccaccggg
     6781 gcaacgtcgg gctgctaccg acgatggcga cgatcccgac gcccggcggc ccgccgcccg
     6841 cgcctccgca accccccatt ccgccgggcc cacgcggcg
//
LOCUS       DASSGQ010000366         5268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1516152,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000366 DASSGQ010000000
VERSION     DASSGQ010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516152"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /gene="dxr"
                     /locus_tag="VFI55_16220"
     CDS             complement(<1..1030)
                     /gene="dxr"
                     /locus_tag="VFI55_16220"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HET7075610.1"
                     /translation="MTNQTIPAAEGRLRVLVLGSTGSIGTQALEVIAANPDRFELVGL
                     AAGGTKLDTLLRQRAETGVTNIAIADDRAARLAGQEVGKVPFHGPEAVTRLVQETDAD
                     VVLNALVGALGLRPTLAALESGARLALANKESLVAGGPLVLRAAQPGQIVPVDSEHSA
                     LAQCLRAGTPDEVAKLVLTASGGPFRGWTAADLEGVTPEQAGAHPTWSMGPMNTLNSA
                     SLVNKGLELIETHLLFGIPYDRIEVVVHPQSIVHSMVTFIDGSTIAQASPPDMKLPIS
                     LALGWPHRIGGAAPACDFTTACSWEFEPLNSDVFPAVELARHAGQTGGCLTAVYNAAN
                     EEAAEAFLQ"
     gene            1172..1426
                     /locus_tag="VFI55_16225"
     CDS             1172..1426
                     /locus_tag="VFI55_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2631 domain-containing protein"
                     /protein_id="HET7075611.1"
                     /translation="MASTEVEHYNGVDVAEVPSAAWGWSKINTRTWQGVGLFSVIFLL
                     AMLRGNHVGHVEDCFLIGFAVLTAVFLVRDLWGRRRGWIR"
     gene            complement(1419..2237)
                     /locus_tag="VFI55_16230"
     CDS             complement(1419..2237)
                     /locus_tag="VFI55_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HET7075612.1"
                     /translation="MPTFATPQPIAVRIEVGSGSIRLAASDRDDTVVDVRPHDESRNS
                     DVWAAEHTRVDFRDGNLVVLGAKRGFPLVRGGAIDLRIALPSHSRLHASLASADLRAE
                     GEFADVQIASASGDVEVDSVAGKLKVAQASGSFTVHAALGGVSVATASGDVTVGDLDG
                     DLKLKAASGSLSVDRLRGHVNSRTASGSVNVAVAVLGGVSARSASGEVAIGVAEGTAV
                     RLDIVSGSGVVTNELQPSDGPEHGDETFVLNVRTGSGEVNIHRANPAHETAGAT"
     gene            complement(2272..2799)
                     /locus_tag="VFI55_16235"
     CDS             complement(2272..2799)
                     /locus_tag="VFI55_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET7075613.1"
                     /translation="MDLQPYVDAVRHELNIAAAAAGTEAQDLADRLSAPLESAMRLAL
                     LEALSAAAEEITRELAPGSVEVRLRGRDPEFMVRASFDEAPDDSAFVAQYDDDRGTWR
                     VTLRLPEGLRSAVEAAARQDGSSLNSWLVRAAAVASQGAGHGSGPAAGHGATTKSDTG
                     RPGRSTGHRVTGWVR"
     gene            complement(2859..3761)
                     /locus_tag="VFI55_16240"
     CDS             complement(2859..3761)
                     /locus_tag="VFI55_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HET7075614.1"
                     /translation="MNHGLIGLAFAAGLVAALNPCGFAMLPAYLLLVVRGQRSGESST
                     SPRALALVGRAMAATVGMALGFVTVFGLFGALTISAASAVQRYLPYATVLIGVLLVAL
                     GIWLLSGRELTALTPRQLGPRWAPQVRQRSRFAGMYGYGVSYAIASLSCTIGPFLAVT
                     AAGFRGGSVLTGVSIYLAYVAGLTLVVGVLAIAAASASSALADRLRRVLPFVNRISGV
                     LLVLVGLYVGYYGVYELRLFSALTTRANPQDAVITAAGRLQGALAGWVHQYGVLPWVV
                     ALSVLLIGAFAGAWYRRARRPHQH"
     gene            complement(3766..4275)
                     /locus_tag="VFI55_16245"
     CDS             complement(3766..4275)
                     /locus_tag="VFI55_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HET7075615.1"
                     /translation="MIIRLFPPLRLVVAAIFAAALLAGLIDAPRAVAADERLQFTGTT
                     LSGAPFNGASLQGKPAVLWFWTPWCPFCNAEAPGVSQVAAANPAVTFVGVAAHSDVAA
                     MQNFVSKYNLNFTTLNDTDGAIWARYNVPWQPAYVFYRADGGSTFVNNPTSAMSQQEL
                     AGRVAALTH"
     gene            complement(4407..>5268)
                     /gene="rlmN"
                     /locus_tag="VFI55_16250"
     CDS             complement(4407..>5268)
                     /gene="rlmN"
                     /locus_tag="VFI55_16250"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET7075616.1"
                     /translation="VADVTCDEGQTRKTLWRALDGATVESVLMQYPQRNTVCISSQAG
                     CGMACPFCATGQGGLTRNLSTAEILEQVRAGAAALRDDFGPPSQRLSNVVFMGMGEPL
                     ANYARVLAAVRRITGTPPDGFGISARSVTVSTVGLVPAIRKLADERLGVTLALSLHAP
                     DDELRDTLVPVNNRWKISEALDAARYYADVTGRRVSIEYALIRDVNDQPWRADLLGKR
                     LHRALGPLVHVNLIPLNPTPGSDWDASPKAAEREFVRRVRSSGVSCTVRDTRGREISA
                     ACGQLAAGGG"
BASE COUNT          872 a         1807 c         1783 g          806 t
ORIGIN      
        1 cttgaaggaa tgcctctgcc gcctcttcgt tggcggcgtt gtagacggcg gtcaggcagc
       61 cgccggtctg gccggcatgc cgggccagct cgaccgcggg gaagacgtcg ctgttcagcg
      121 gctcgaattc ccagctgcag gcggtagtga agtcgcaggc aggagcggcg ccgccgatcc
      181 ggtgcggcca gcccagggcc agcgaaatgg gcagcttcat gtccgggggg ctggcttggg
      241 cgatggtcga accgtcgatg aacgtgacca ttgagtgaac gatcgactgc gggtgtacga
      301 cgacctcgat gcggtcgtag gggatgccga acaacaggtg cgtttcgatg agctccaagc
      361 ccttgttgac cagcgacgcc gagttcaggg tgttcatcgg ccccatcgac caggtcggat
      421 gggcgcccgc ttgctcgggg gtcacgccct cgaggtcagc ggcggtccac ccgcgaaacg
      481 gcccgccgga ggccgtcagc accagcttgg cgacctcgtc gggcgtgccg gcgcgcaggc
      541 actgagccag cgcggagtgt tcggagtcga cgggcacgat ctggcccggc tgcgccgccc
      601 gcaacaccag cggccccccg gcgaccagcg actccttgtt ggccagcgcc agtcgggccc
      661 ctgactccag cgcggccagt gtcggtcgca ggcccaacgc cccgaccagc gcgttgagga
      721 cgacgtcggc gtcggtttcc tggaccaggc gggtgaccgc ctcgggtccg tggaagggga
      781 ctttcccgac ttcttggccg gccagccgcg cggcgcggtc gtcggcgatg gcgatgttgg
      841 tgaccccggt ctccgcccgc tgccgcagca gggtgtccag ttttgtgccc cctgcggcca
      901 gcccgaccag ctcgaatcgg tccggattgg ccgcgatgac ctccagcgcc tgggtcccga
      961 tcgagccggt gctgcccaac accagcacgc gtaggcggcc ctcggccgcc gggatcgtct
     1021 ggttggtcac tcactcattg tgccgtcccg cgacgccgcg ggccatgaag cggaccgccg
     1081 tacgcgggac ggcacgatcg ctgtcgggcc tggcagtggt gtgccagaat ctccatgcag
     1141 agcagccgat ccgatccgaa ggggattcgc cgtggccagc accgaggtgg agcactacaa
     1201 cggcgtcgac gtcgccgagg tcccgtcggc ggcatggggg tggagcaaaa tcaacacccg
     1261 cacctggcag ggagtcgggc ttttctcggt catcttcctg ctggcgatgc tgcgcggcaa
     1321 ccacgtcggc cacgtcgagg actgcttcct gatcggattt gccgtgctca cggccgtttt
     1381 cctggtgcgc gacttgtggg gccgccggcg cggctggatc aggtagctcc agccgtctcg
     1441 tgcgccgggt tcgcccgatg gatgttcacc tcaccggagc cggtacgcac gttgaggacg
     1501 aaggtttcgt cgccgtgctc ggggccgtct gacggctgca attcgttggt gacgacgccg
     1561 gatccgctga cgatgtccag ccggaccgcg gtgccctcgg caacgccgat cgcgacctca
     1621 ccgctggcgc tgcgggccga cacgccgccg aggacggcga cggccacgtt gaccgatccc
     1681 gacgcggtcc gcgaattcac gtgaccgcgc agccggtcga cgctcagcga gccgctggcg
     1741 gccttgagct tcaggtcgcc gtcgaggtcg ccgacggtca cgtcgccgga agctgtggca
     1801 accgaaacac cgcccagtgc ggcatgcacg gtgaacgacc cggatgcctg ggccactttg
     1861 agctttcccg cgacggagtc gacttcgaca tcgccgctgg ccgaagcaat ctggacgtcg
     1921 gcgaattctc cctcggcacg cagatcggcc gacgcgagcg atgcgtgcag ccgcgaatgt
     1981 gacggcagcg cgatccgcag gtcgatggcc ccgccccgga ccagcgggaa gccgcgtttg
     2041 gcgcccaaaa cgaccaggtt gccgtcgcgg aagtcgaccc gggtgtgttc ggcggcccag
     2101 acgtcggagt tgcgtgattc atcgtggggc cgcacgtcca ccacggtgtc gtcgcggtca
     2161 ctggccgcga gccggatcga cccgctgccg acttcgatcc gcacggcgat cggctggggg
     2221 gtagcaaagg tcggcatttc tcgtctcctt cgtcgatggt gtcaggtggt gtcaacgcac
     2281 ccagccggtc acgcggtgac cggtggatcg gcccgggcgg ccggtgtcgg atttggtggt
     2341 ggcgccgtga ccggccgctg gaccggaccc gtgacccgcc ccttgactgg ccacagccgc
     2401 tgcccgcacc agccaggagt tcaacgacga cccgtcctgg cgagcggccg cctcgaccgc
     2461 agaccgcagc ccttcgggca accgcagagt gacccgccag gtgccgcggt catcgtcgta
     2521 ctgggcaaca aatgcgctgt catcaggcgc ttcgtcgaac gaagcgcgga ccatgaactc
     2581 ggggtcgcgg ccgcgcagcc ggacctccac ggatccgggt gccagctcgc gggtgatctc
     2641 ttcggcggcg gccgacagcg cttccagcag cgccagccgc atcgccgatt ccagcggagc
     2701 gctcagccgg tcggccaagt cctgcgcttc cgtgccggcg gccgccgcgg cgatgttcag
     2761 ctcgtggcga acggcgtcga catacggttg caaatccata gtgtcatggt gacatcactt
     2821 tgatgtcatt acaagtgtcg caatggtgca ggatggcgtc agtgttgatg cgggcgccgc
     2881 gcccgccggt accaggcgcc ggcgaacgcg ccgatcagca gcacggacag cgcgaccacc
     2941 catggcagga cgccatattg gtgcacccag ccggctagcg cgccttgcag gcgacctgcc
     3001 gcggtgatca cggcatcctg gggattcgcg cgggtagtca gggcactgaa gagacgcagc
     3061 tcgtagacgc cgtagtagcc gacatacagg ccgaccagca ccagcagcac gccgctgatc
     3121 cggttgacga acggcaatac tcgccgcagg cggtcggcca gagccgagct ggccgacgcc
     3181 gccgcgatgg caaggacgcc cacgacgagg gtcaggcccg cgacgtaggc gagatagata
     3241 gacacgcccg tgaggacgga cccaccccgg aacccggcgg cggtgaccgc cagaaacggc
     3301 ccgatagtgc acgacaggga ggcgatcgca tagctgacgc cgtacccgta catacctgca
     3361 aaccgagacc gttgccgcac ctgcggggcc cagcgcgggc cgagctggcg cggcgtgagc
     3421 gccgtcagct ctcgacccga tagcagccaa atccccaggg cgacgagcaa tacaccgatt
     3481 agcacggtgg cgtagggcag gtaccgctgt accgcggacg ccgccgagat ggtcagcgca
     3541 ccgaacaatc cgaacaccgt cacaaagccg agcgccatgc cgaccgtggc tgccatcgca
     3601 cggccgacca gcgccaatgc ccgcggcgac gtcgacgatt cgccagaccg ctgcccgcgt
     3661 accaccagaa ggaggtacgc gggcagcatg gcgaacccac aggggttcag cgctgccacc
     3721 aatccggccg cgaacgcaag accgatcagg ccgtggttca cggggtcagt gggtcagcgc
     3781 cgccacccgg cccgccagct cctgctggga catcgccgag gtggggttgt tcacgaacgt
     3841 cgagccgccg tccgcccggt agaacacgta cgccggctgc caagggacgt tgtagcgggc
     3901 ccagatcgcg ccgtcggtgt cgttgagagt agtgaaattc aggttgtact tggagacgaa
     3961 gttctgcatc gcggcgacgt ccgagtgcgc cgcgacgccg acgaaggtga cggccggatt
     4021 tgccgccgcc acctggctga cgccgggagc ttcggcattg cagaacggac accacggcgt
     4081 ccagaaccac agcaccgcag gcttcccctg caggctggcg ccattgaacg gcgcaccgct
     4141 gagcgtcgtt ccggtgaact gcagacgctc gtcggcggct accgcgcgcg gcgcatcgat
     4201 cagcccggcc agcagtgcag ccgcgaaaat ggcggccaca accaatctga gcggagggaa
     4261 caggcgaata atcatgtggc atacctcgtt tgctggtgtt atgcattaca cgacgggata
     4321 gccggctttc gggttcatcg gccccccgct acccgcctcc ggcggccagc tgtccgcagg
     4381 cccagcccga ttcgcctgcg ctccagctac ccgcctccgg cggccagctg tccgcaggcg
     4441 gcgctgatct cacggccgcg cgtgtcgcgc accgtgcatg acaccccgct ggaacggacg
     4501 cgccggacga attcccgttc cgcggccttg gggctggcat cccaatcact gcccggcgtc
     4561 gggttgagcg ggatcaggtt cacgtgtacc agcggcccga gggcgcggtg tagtcgctta
     4621 cccagcagat cggcccgcca tggttggtcg ttgacgtcgc ggatcagtgc gtactcgatc
     4681 gatacccgcc gaccggtcac atcggcgtag taccgggccg catcgagggc ctcactgatc
     4741 ttccaccgat tgttgaccgg aaccagggtg tcgcggagtt cgtcgtcggg tgcgtgtaac
     4801 gacagcgcca acgtcacccc gagccgttcg tcggccagtt tgcggatcgc cggaaccaag
     4861 cccaccgtcg acactgtcac cgagcgcgcc gatatcccga acccgtcagg cggcgtcccg
     4921 gtgatccgtc gcaccgccgc gagcaccctg gcgtagttgg ccagcggctc ccccatgccc
     4981 atgaagacga cattcgacag ccgctgactg ggcgggccga agtcgtcgcg caacgccgcg
     5041 gcgcccgcac gcacctgctc cagaatttcg gcggtcgaca ggttgcgggt caacccgccc
     5101 tgaccggtcg cacagaacgg acaggccatg ccgcagccag cctgcgacga gatgcacacc
     5161 gtattgcgct gcggatactg catcaggacc gattcgacgg tggcaccgtc gagggctctc
     5221 cacaacgtct ttcgcgtctg gccttcatcg cacgtgacat cggcgacc
//
LOCUS       DASSGQ010000367         7437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_757334,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000367 DASSGQ010000000
VERSION     DASSGQ010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7437
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757334"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2040)
                     /locus_tag="VFI55_16255"
     CDS             complement(<1..2040)
                     /locus_tag="VFI55_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7075617.1"
                     /translation="MISGELLTGRDGELGIIRRALSGAGNRSGVVIAGAAGVGKTWLA
                     REVLRRAEAAGERTKWIVGTESAHALPLGAFIGSLGEAMSDPLTNVRRVINSYVAQQR
                     RGRVVVGIDDAHLLDALSALVVHELAQSGGTRLVVTVRTGSDEPDAVTALWKDGLLAR
                     LDLEPLSAAATREVIESALGGPVDARSAARFRRLTGGNSLFLRQLLADQIAAGRMRNS
                     AGVWMWDGDVAVSASLSDTVARQMGRLNPRIALVVDTLSQCEPLAVDVLCDLVRRRDL
                     AVAEGMGLVSVERTGGGLMARLAHPLFGELRRASAGEMYLSAIRGRLATRLAETGDTD
                     MRAIVRRGLLTLESDLDPEPELYLKSARHAMTLLDLDLADRFATAATQAGAPGASGVR
                     AMNLFLLGRGEQTETVLRDMVDRPEDCVPDCHHWATVRAANLVWSLANPADAAVILNG
                     LASPNSKAPETPEQVAERLAVQACVDAVSARCELAAERARAALDSPDLSDFHAMMASV
                     ALIMAMGALGEVDELAGVAEQALRRATTSFQASHLRFWCAAVYGRACRLTGRIDEFVS
                     TAKQVADSARDVPGLAYANLAFLLGNAELARGAVADSARLVHEAVAGVQINSITTGLR
                     PASYFALAEAHAKLGQPGEANDAVARARAAVPPDFLFMDTALSLAGGWAMVASGCL"
     gene            2193..3686
                     /locus_tag="VFI55_16260"
     CDS             2193..3686
                     /locus_tag="VFI55_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7075618.1"
                     /translation="MAFVQLNSSVEAMGDVRMKPDSKANVGEHAVVLGASVAGLLAAR
                     SLADFFDTVTVVERDALTGHLTADDATRRGVPQGRHVHALLARGAQVIEEFFPGVLGE
                     LVRDGAQYFDGRDLSRLYYSVGGHLMVRTGSAPSFTGYGATRPFLEAHIRGRVRDIPN
                     VSLLDEHDIVELTSTPDHRRVTGARVVSRRSGAHATLHADLVVDATGRASRTPAWLEG
                     LGYHHPTEDRVVMQLTYASQLLRMPADAPHELGFLIGVAPGRPRGVGLFRCENDLWMF
                     TVMGVAGHQPDQDLAAMCEFVEDFAPAQLLAAVRGAQPVGQPARHKTPCSRWRRYDQM
                     QRFPEGLLVTGDAVCSFNPIYGQGMTLAALEASVLRDCLSCGTEDLARRFFLASAAPI
                     RQAWQLSTNSDLALPEIGGTPPLVARLLNRYVESVLSAAESDAVVVNQFGRVTSLVDP
                     ATRLLRPKFVWRMARANYRCRRRAIAQDEDHNEGHIEGHIAFADATP"
     gene            complement(3697..4695)
                     /locus_tag="VFI55_16265"
     CDS             complement(3697..4695)
                     /locus_tag="VFI55_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET7075619.1"
                     /translation="MRERLVIAVLALAVSLFASPPTASAQAAGFDLQAHRGGRGETTE
                     ESLRAFAKSLELGVSTLELDIVITKDGQPMVWHDPTIDATKCADTGPAFAGDPAYPYV
                     GKLVHELTLAQIGTLDCGRQLADFPRAEVVRGNKIATLPQVFALADSYQAGYPAGLRY
                     NIETKVEADQPGRSADPQQFVDVILAAVRSAGEVDRVEIQSFDWRTLPMVHQAEPSIP
                     LVALYDETTWVPDSPWLGGVNPANPALGGDPALGALLVGANMVSPNYTLVTGKPYVDH
                     AHSLGLKVIPWTVDDADAMREQIAYGDDGIITDYPTLLRGVLAELGMPLPPAYHRD"
     gene            complement(4701..5630)
                     /gene="rlmB"
                     /locus_tag="VFI55_16270"
     CDS             complement(4701..5630)
                     /gene="rlmB"
                     /locus_tag="VFI55_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET7075620.1"
                     /translation="MAGNSRRQGAVRKSGTKKGPTVGSGGQRRRGLEGRGPTPPAHLR
                     PNHPAAKRAQPQPRRPAKRADETETVLGRNPVLECLRAGVPATALYVALGTEADERLT
                     ESVARAADLGIAILEVPRTDLDRMTTNHLHQGIALQVPPYNYAHPDDLLAAAAATPPA
                     LLVALDNISDPRNLGAIVRSVAAFGGHGVVIPQRRSASVTAVAWRTSAGAAARIPVAR
                     ATNLTRALKNCADQGLRVIGLDAGGDTAIDELDGTDPLVVVVGSEGKGLSRLVRQTCD
                     EVVSIPMAGHAESLNASVAAGVVLAEIARQRRS"
     gene            complement(5631..7037)
                     /gene="cysS"
                     /locus_tag="VFI55_16275"
     CDS             complement(5631..7037)
                     /gene="cysS"
                     /locus_tag="VFI55_16275"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET7075621.1"
                     /translation="MTDRARLRLHDTAAGAVRDFVPLRPGHVSIYQCGATPQGLPHIG
                     HVRSGVAFDILRRWLIARGYDVAFIRNVTDIEDKILAKAAAAGRPWWEWAATHERAFT
                     AAYDALDVLPPSAEPRATGHISQMIELMDRLIETGHAYAGGGDVYFDVLSYPEYGQLS
                     GHRIDDVHQGEGVATGKRDQRDFTLWKGEKPGEPSWPSPWGRGRPGWHIECSAMARAY
                     LGPEFDIHCGGMDLIFPHHENEIAQSRAAGDGFARYWMHNGWVTMGGEKMSKSLGNVV
                     SIPAMLQRVRPAELRYYLGSAHYRSMLEFSDTALQDAVKAYVGVEEFLHRVRGRVGAV
                     EPGEPTPKFAEALDDDLAVPAALAEVHHTRAEGNRALDAGDHQDALARAGAIRAMMGI
                     LGCDPLDERWESRDETSAALAAVDVLVQAELQKREEAREKRNWALADEIRDRLKYAGI
                     EVTDTADGPQWTLGGDGK"
     gene            complement(7100..>7437)
                     /locus_tag="VFI55_16280"
     CDS             complement(7100..>7437)
                     /locus_tag="VFI55_16280"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HET7075622.1"
                     /translation="CDALLSAAGLGDVGAVFGVGDPRWEGVSGADMLRHVVDLISEHG
                     YRVGNAAVQVIGNQPKIGPRRVEAQQLLSGLLRAPVSASATTTDGLGLTGRGEGLAAI
                     ATALLVPVG"
BASE COUNT         1162 a         2636 c         2486 g         1153 t
ORIGIN      
        1 caagcagccg cttgccacca tcgcccaccc gccggccaac gataacgccg tatccatgaa
       61 cagaaagtcg ggcggcacgg ccgcccgcgc cctggcgacc gcgtcattcg cctcgccggg
      121 ctgtccgagc ttggcgtgcg cctccgccag cgcgaagtag ctcgccggcc gcaaacccgt
      181 tgtgatggag ttgatttgga ccccggcaac tgcttcgtgc accagccggg ccgagtcggc
      241 gacggcgccg cgagccagtt cggcgttccc caacaggaag gccaggttcg cataggccag
      301 accgggtacg tctcgggccg agtcggccac ttgctttgcg gtgctgacga attcgtcgat
      361 acgcccggtc agccggcagg cgcggccgta cacggcggcg caccagaagc gcaggtggga
      421 cgcctgaaac gacgtcgtcg cgcgccgcag cgcctgttca gcaacgccgg cgagctcgtc
      481 gacctccccc agcgcgccca tggccatgat cagcgcaacc gacgccatca tcgcgtgaaa
      541 gtccgacaaa tcgggggaat ccagtgcggc ccgggccctt tcggccgcga gctcgcagcg
      601 cgccgacacc gcgtccacgc aggcctgcac cgccagacgc tccgccacct gttccggcgt
      661 ctcgggcgcc ttggaattcg gggaggcaag accgttgagg atcaccgccg cgtcggcggg
      721 gttggcgagc gaccacacca ggttggccgc ccgcaccgtc gcccagtgat gacagtcggg
      781 cacgcaatcc tcggggcgat cgaccatgtc gcgcaacacc gtctcggtct gctcgccgcg
      841 tccgagcagg aacagattca tcgcccgcac cccggacgcc ccgggcgccc cggcctgagt
      901 ggcggcggtg gcgaaccggt cggcaagatc cagatccagc agcgtcatgg catgccgtgc
      961 cgatttcaga tacagctcgg gctcgggatc gaggtcggac tcgagggtca gcagcccgcg
     1021 gcgcacgatg gcccgcatgt cggtgtcacc ggtttcggcc agccgtgtgg ccagcctgcc
     1081 ccggattgcc gacaagtaca tctcgccggc gctcgcgcgg cgaagctcgc cgaacagcgg
     1141 gtgcgccagc ctggccatca gcccgccgcc ggtgcgttcg acgctcacca ggcccatccc
     1201 ctcagcgacg gccaggtcgc ggcggcgcac caagtcgcac agcacatcga cggccaacgg
     1261 ctcacactgc gacagcgtgt cgaccaccag cgcgatccgc gggttgagcc gacccatctg
     1321 gcgcgccacc gtgtcggaca ggctcgccga gaccgcgacg tcgccgtccc acatccatac
     1381 accggccgaa ttccgcatcc gcccggccgc gatctggtcg gccagcagct gccgcaagaa
     1441 cagcgagttc ccaccggtca accgtcggaa ccgggcggcg ctgcgggcgt cgaccggacc
     1501 gcccagcgcg ctctcgatca cctcgcgcgt cgccgcagcc gacagcggtt cgaggtcaag
     1561 gcgggccagc agcccgtcct tccacagcgc ggtcaccgcg tcgggttcgt cgctgccggt
     1621 gcgcaccgtg accaccagcc gggtcccgcc cgactgcgcc agctcgtgca cgacgagggc
     1681 ggacagcgcg tccagcaggt gcgcgtcatc gatgcccacc accacccgac cccgacgctg
     1741 ttgggcgacg taggagttga tcacgcgccg cacgtttgtc agcggatccg acatcgcttc
     1801 gcccagcgag ccgatgaatg cgccgagagg cagggcatgc gccgattcgg tgccgacgat
     1861 ccatttcgtg cgctcgcccg ccgcctcggc gcgccgcagc acttcgcggg ccagccaggt
     1921 cttgcccaca ccggccgctc cggcgataac cacgccggag cggttgccag ctccacttag
     1981 ggcacggcga atgatcccta gctcaccatc gcgtccggtc agcagctcgc cgctgatcac
     2041 gcggcgacta tagccggtag atcggcgttg ggtgggccgg attgtgcacg tgttcgatga
     2101 gcttttcggg cgaccggcgt gtggtgcgcg cgtatccggg ccacaggtcg ggtagtcgcc
     2161 tacgctatgc gcgcccagga tgcgctgtga gcatggcttt tgtgcagcta aacagctcgg
     2221 tggaggcgat gggcgatgtt cgaatgaagc ccgacagtaa agcaaacgtc ggcgagcacg
     2281 cggtggtgtt gggagcgagc gtggccggtc tgctggcggc ccggtcgctc gccgatttct
     2341 tcgacacggt cacggtggtg gagcgcgacg cgctgacggg acacctaacc gccgacgacg
     2401 ccactcgccg aggtgtgccg cagggccggc acgtacacgc cctgctggcc cgcggcgcgc
     2461 aggtgatcga ggaatttttc cccggcgtgc tcggcgagtt ggtccgtgac ggcgcgcagt
     2521 acttcgacgg ccgggacctg tcacggctgt actacagcgt gggcggacac ctgatggtgc
     2581 gcaccggcag cgcgccctcg ttcaccggtt acggcgcaac ccggccattc ctggaggcgc
     2641 atatccgtgg ccgagtccgc gacatcccca atgtcagcct gctcgacgag cacgacatcg
     2701 tggagttgac ctcgacgccc gaccatcgac gcgtcaccgg agcacgggtg gtcagccgcc
     2761 gcagcggagc gcacgccacg ctgcacgccg acttggtggt cgatgccacc gggcgcgcct
     2821 cccgcacccc ggcgtggctg gagggcctgg ggtaccacca cccgaccgag gaccgggtgg
     2881 tgatgcagct gacgtacgcg agccagctgc tgcgtatgcc cgcggatgca ccgcacgaac
     2941 tcggcttcct catcggggtc gcaccgggca ggccgagagg ggtcggcctg ttccgctgcg
     3001 agaacgacct gtggatgttc accgtgatgg gcgtagccgg tcaccagcct gaccaggacc
     3061 ttgcggcaat gtgcgagttc gtcgaggact ttgcccccgc ccaactcctc gccgcggtgc
     3121 gcggtgcaca acccgtcggc caaccggcgc ggcacaagac gccctgcagt cgatggcgcc
     3181 gttacgacca gatgcagcgc ttccccgaag gcctgctggt caccggcgac gccgtatgca
     3241 gcttcaaccc gatctacggc cagggtatga cgcttgccgc actcgaggcg tcggtgctgc
     3301 gtgactgttt atcctgcggc acagaggatt tggcacggcg gttcttcctc gcttcggcag
     3361 caccgatccg gcaggcctgg caactgtcga ccaactccga cctggctctg cccgaaatcg
     3421 gcggcactcc accgctggtc gcgcggctgc tcaacagata cgtcgaatcc gtgctcagcg
     3481 cagccgaatc cgacgccgtg gtcgtcaatc agttcggcag agtgacctcg ctggtggatc
     3541 ccgccacccg gctgttgcgc ccaaagttcg tatggcgcat ggcccgcgcc aactatcgct
     3601 gtcgccggcg tgccatcgca caggacgagg atcataacga gggacacatc gagggacaca
     3661 tcgccttcgc cgacgcgacg ccgtgagagc ggctacctaa tcccggtggt aggccggcgg
     3721 cagcggcatg ccgagctcgg ccagcacacc gcgcagcagc gtggggtagt cggtgatgat
     3781 gccgtcgtcc ccgtaggcga tctgctcgcg catggcgtcg gcatcatcga cggtccaggg
     3841 aatgaccttg agccccaacg aatgcgcgtg atcgacatag ggtttgccgg tgaccagcgt
     3901 gtagttgggc gataccatgt tggcgcccac cagtagcgcc ccgagcgccg ggtcaccccc
     3961 taacgccggg ttggcggggt tgacgccgcc cagccacggc gaatccggca cccaggtcgt
     4021 ctcgtcatac aaggccacca acggaattga cggctctgcc tggtgcacca tcggcaacgt
     4081 tcgccagtcg aagctctgaa tctcgacgcg atcgacctcg cccgcggacc tcaccgcggc
     4141 cagtatcacg tcgacgaact gctgggggtc cgccgatcga cccggctgat cggcctccac
     4201 cttggtttcg atgttgtagc gcaaccccgc cgggtacccg gcctgatagg agtcggcgag
     4261 cgcgaatacc tgcggcaagg tcgctatctt gttgccccgc accacttccg cccggggaaa
     4321 gtcggcgagc tgccgaccgc agtcgagggt accgatctgc gccagcgtca gctcgtgcac
     4381 gagctttccg acgtagggat acgcggggtc gccggcgaac gcggggccgg tgtcggcgca
     4441 cttcgtcgcg tcgatcgtcg ggtcgtgcca caccatgggt tggccgtcct tggtgatgac
     4501 gatgtcgagt tcgagtgtgc tgacgccgag ttcgagcgac ttggcaaacg cgcgcagcga
     4561 ttcttcggtg gtctcgccgc gcccgccgcg atgggcctgc aggtcgaaac ctgctgcctg
     4621 cgcggatgcc gtcggcggcg acgcgaaaag cgagacggcc agcgccagga cggcgataac
     4681 caagcgttca cgcatcggcg tcagctccgg cgctgccggg cgatttcggc gagcaccacc
     4741 ccggcggcca ccgacgcgtt gagcgattcg gcgtggccgg ccatcggaat cgacaccacc
     4801 tcgtcgcagg tttgccgcac cagacgggac agccccttgc cttccgaccc cactacgacc
     4861 accagtgggt ccgtcccgtc caattcgtcg atcgcggtgt cgcccccggc gtccaacccg
     4921 atcacccgaa gcccttgatc ggcacagttt ttcagcgccc tcgtgaggtt ggtggcccgc
     4981 gccaccggga tccgggcggc cgccccggca ctggtgcgcc acgccaccgc cgtcaccgag
     5041 gccgaacgcc gctgcgggat taccacgccg tggccgccga acgccgccac cgaacgaacg
     5101 atcgcaccga ggttgcgcgg gtcggagata ttgtccagcg ccaccaacag cgccggcggc
     5161 gttgcggcgg cggccgcgag cagatcatcg gggtgggcgt agttgtacgg cgggacctgc
     5221 aaggcgatgc cctgatgcag gtggttggtg gtcatccggt ccaggtcggt acgcggtacc
     5281 tccaggatcg cgatgcccaa atcggccgcg cgggcgacgg attcggtgag ccgctcgtcg
     5341 gcttcggtac ccagggcgac gtagagcgca gtggccggca caccggcacg caggcactcc
     5401 agcaccggat tgcggcccag caccgtttcg gtctcatcgg cgcgcttagc cggccgacgc
     5461 ggctgaggct gggcgcgctt ggccgccggg tgattggggc gcaggtgcgc ggggggcgtg
     5521 gggccgcgcc cctccagccc gcggcggcgc tggccgcccg agccgacggt ggggcccttc
     5581 ttggtgccgg atttgcgcac cgcgccctgg cgccgcgagt tgccggccat ctacttgccg
     5641 tcaccaccca acgtccactg cggtccgtcg gcggtatcgg tgacctcgat gccggcgtac
     5701 ttgaggcgat cccggatctc gtcggcgagt gcccagttgc gcttctcgcg cgcctcttcc
     5761 cgcttttgca actcggcctg caccagcacg tcgacggcgg ccagagcggc agaggtctcg
     5821 tcgcgggatt cccagcgctc atcgaggggg tcgcagccca ggatgcccat catcgcccgg
     5881 atggcgccgg cacgcgccag tgcgtcttgg tggtcgccgg cgtccagcgc gcggttgcct
     5941 tcggcgcggg tgtggtgcac ttcggccagc gcggccggga ccgccagatc gtcgtcgagc
     6001 gcctcggcga acttcggggt cggttcgccg ggctcgacgg caccgacccg gccgcggaca
     6061 cggtgcagaa actcctcgac accgacatag gccttgaccg cgtcctgcag ggcggtgtcg
     6121 gagaactcca gcatcgaccg gtaatgagcg ctgcccaggt agtagcgcag ttcggccggt
     6181 cgcacccgct gcagcatcgc cgggatggac accacgttgc ccagcgactt gctcatcttc
     6241 tcgccgccca tcgttaccca accgttgtgc atccagtagc gggcgaatcc atccccggcg
     6301 gcgcggcttt gcgcgatttc gttctcgtgg tgcgggaata tcaaatccat tccaccgcaa
     6361 tggatgtcga attccggccc gagataggcg cgggccattg ccgagcattc gatatgccag
     6421 cccggacgtc cgcgccccca cggcgagggc cacgacggtt cgccgggctt ttcgcccttc
     6481 cacagggtga agtcgcgctg gtcacgcttg ccggtcgcca caccctcgcc ttgatggacg
     6541 tcgtcgatcc ggtgcccgga cagttggccg tactccgggt agctgagcac gtcgaagtag
     6601 acgtcgccgc cgccggcata ggcgtggccc gtctcgatca ggcgatccat cagttcgatc
     6661 atctggctga tgtgcccggt ggcgcggggc tccgccgacg gcggcagtac gtccaatgcg
     6721 tcataggccg cggtgaacgc ccgctcatgg gtggccgccc actcccacca cggccggccc
     6781 gccgcggccg ccttggccaa aatcttgtct tcgatgtcgg tcacgttgcg gatgaatgcg
     6841 acgtcgtagc ctcgcgcgat cagccagcgg cgcaggatgt cgaaggccac accgctgcgg
     6901 acatgcccga tgtgcggtag tccctgcggg gtggcgccgc actggtagat ggagacgtgc
     6961 ccgggccgca gcggaacgaa atcgcgcacg gcaccggccg ccgtgtcgtg tagccgcaaa
     7021 cgggcgcgat cggtcacggc gtgccagctt acctggccaa ttgccgcaac cagcgaccgc
     7081 gagtgccccg gccggtcagc tacccgacgg ggaccagcag cgcggtggcg atcgcggcca
     7141 ggccctcgcc tcgaccggtc aggccgagcc cgtcggtggt cgtcgccgac gccgataccg
     7201 gcgcccgcag cagaccggac aacagctgct gcgcctcgac gcggcgggga ccgatcttcg
     7261 gctggttgcc gatcacctgc actgcggcgt tgccgacgcg atagccgtgt tccgatatca
     7321 ggtcgacgac gtggcgcagc atgtcggcgc cgctcacgcc ttcccaacgc ggatcgccaa
     7381 ccccgaacac ggcgccgaca tcgcccaggc ccgcggcgga caacagcgca tcgcaca
//
LOCUS       DASSGQ010000368         7234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_726760,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000368 DASSGQ010000000
VERSION     DASSGQ010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7234
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726760"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VFI55_16285"
     CDS             <1..247
                     /locus_tag="VFI55_16285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7075623.1"
                     /translation="ELTELHIHPRAQGRGLGEALARRLLAGRAEENVLLSTPEINGEP
                     NRAWRLYRRLGFTDIIRGYRFTGDPRAFAILGRALPL"
     gene            450..1103
                     /locus_tag="VFI55_16290"
     CDS             450..1103
                     /locus_tag="VFI55_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3153 domain-containing protein"
                     /protein_id="HET7075624.1"
                     /translation="MLLLLVPLATACLRVRASITVSPDDLVSGEIIAAAKPKTPKDTG
                     PQLDTNNLPFSQKVAVSNYDSDGYVGSQAVFSDVTFAELPQLANMNSNAAGVNLSLRR
                     NGNLVILEGRADLTSVTDSDADVELTVAFPGVVTSTNGDRVESDVVSWKLKPGVVSTM
                     TANARYTDPNTRNFTGAGIWLGVASFAVAGVVALLAWMSRDRSPRFTAGGDQPPSSG"
     gene            complement(1100..1996)
                     /locus_tag="VFI55_16295"
     CDS             complement(1100..1996)
                     /locus_tag="VFI55_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycobacterial-type methylenetetrahydrofolate
                     reductase"
                     /protein_id="HET7075625.1"
                     /translation="MTLNTIALELVPPNVADGRERALEDARKVVRHSADFGLEGRIRH
                     AMIPGIIAEEDDRPVAMEPKLDVLDFWSIIKPELPGFRGLCTQVTAFMDDATLGRRLS
                     DLSAAGMEGVVFVGVPRTMNDGEGSGVAPTDALSIYREQVANRGVIVIPTRDSEQGRL
                     NFKCDQGATYGMTQLLYSDAIVGFLTEFAKNTDHRPEILLSFGFVPKVESRIGLINWL
                     IQDPGNAAVADEQAFVKRLAGGEPAEKRRLMVDLYKQVIDGVAELGFPLSIHLEATYG
                     NSGPAFQTFAEMLAYWSPAPEV"
     gene            2261..3310
                     /gene="idsA2"
                     /locus_tag="VFI55_16300"
     CDS             2261..3310
                     /gene="idsA2"
                     /locus_tag="VFI55_16300"
                     /EC_number="2.5.1.1"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004161 - dimethylallyltranstransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0033384 - geranyl diphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (2E,6E)-farnesyl/geranyl
                     diphosphate synthase"
                     /protein_id="HET7075626.1"
                     /translation="MTGAITEQLRRYLQDRRAETAYIGGDYETLTAALEDFVLNGGKR
                     LRPAFAYWGWRAVAPADPDPEALRLFSALELLHACALVHDDVLDESSTRRGRPTTHVH
                     FAALHRDRGWRGSPDRFGTSAAILLGDLALAWADDIVFALELPPEALRRVHRVWADIR
                     TEVLGGQYLDIVAEASAAESVASAMNVDIYKTACYTVARPLQLGVAAAADRPDVQAVF
                     GQFGTDVGVAFQLRDDVLGVFGDPAVTGKPSGDDLRSGKRTVLLAEAVELATRSDPVA
                     ANLLRSSIGAHLTDAQVAQLRDVIESVGALAAAEQRIAALTRRALATLAAAPIDAAAK
                     AGLSELVRMATNRSA"
     gene            3315..4871
                     /locus_tag="VFI55_16305"
     CDS             3315..4871
                     /locus_tag="VFI55_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-(1->6)-mannopyranosyltransferase A"
                     /protein_id="HET7075627.1"
                     /translation="MTTPADSSMHSPAADSQPTKFSVAQHFSRLRAFATTAEARPAKL
                     GLLGSVLITLGGLGAGSTKPHDPLLEALHLSWLRFGHGLVLSSIVLWTGVGLMLIAWV
                     QLGRHTLAGKTSEFTMRATTGFWLAPLLLSVPVFSRDTYSYLAQGALLRDGFDPYAVG
                     PIANPNVLLDNVSPIWTITTAPYGPAFILVAKLVTMIVGNHVIAGTMLLRLCMLPGLA
                     LLMWAAPRMAHHLGTSGALALWICVLNPLVLIHLMGGVHNEMLMVGLMAAGIALTFQG
                     RHVLGVVLITVAIAVKATAGISLPFLVWVWMRHLRDQRGYRPARAFLVATTTSALIFA
                     VVFGILSAVAGVGLGWLTALAGSVKIINWLSVPTAVANLIHALCGRFFPVDFYATLRV
                     TRLIGVVIIAVSLPILWWRFRRDDRAALTGIAWSMLVVVLFVPAALPWYYSWPLAVAA
                     PLAQSRRAVAAIAGLSTWVMVIFKPDGSHGMYSWLHVSLATACALAAWYALHRSPEPV
                     ADRDRKPVAA"
     gene            complement(4880..5284)
                     /locus_tag="VFI55_16310"
     CDS             complement(4880..5284)
                     /locus_tag="VFI55_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2175c family DNA-binding protein"
                     /protein_id="HET7075628.1"
                     /translation="MSSIPAGDDVLDPEEPTYDLTRVAQLLGVAVSKVHQQLREGHLL
                     AVRRGGGVVVPQVFFTNSGEVVKSLPGLLTILHDGGYHETEIVRWLFTPDPSLTVTRD
                     GTRDAISNARPVDALHAHQAREVVRRAQAMAY"
     gene            5353..6570
                     /locus_tag="VFI55_16315"
     CDS             5353..6570
                     /locus_tag="VFI55_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7075629.1"
                     /translation="MAGAGTGDPLEGTLLDGRYLVQSKIGSGGTSTVYRGLDVRLDRP
                     VALKVMDSRYAGDDQFLTRFQLEARSVARLKNPGLLAVYDQGLDARHPFLVMELVEGG
                     TLRELLTERGPMPPHAVVAVLRPVLGGLGAAHRAGLVHRDVKPENVLISDDGEVKIAD
                     FGLVRAVAAAGITSASVILGTAAYLSPEQVRDGNAGPRSDVYSTGILTYELLTGRTPF
                     TGDSTLSIAYQRLDTDVPPPGAAIDGVPPQFDEFVRRATTRDSAVRYADALEMGAELD
                     AIADELALPDFRVPAPRNSAQHRSAALHHSRMSQRPPVAAPVPHPTRQLTRGPGDWSE
                     PRRPADLDAEPDDYEYEAISGEFAGIAMSEFVWARQHARRMVLVWVAVVLAATGLVAT
                     AAWTIGSNLRGLL"
     gene            complement(6613..>7234)
                     /locus_tag="VFI55_16320"
     CDS             complement(6613..>7234)
                     /locus_tag="VFI55_16320"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET7075630.1"
                     /translation="AMLRLSDAEDGEQQLYDLSAHTVWIGERTRQLDGAHVAFAEVIA
                     NPIGVKIGPTMTPEVAVEYVERLDPHNKPGRLTLVTRLGNNKVRDLLPPIVEKVQATG
                     HQVIWQCDPMHGNTHESSNGYKTRHFDRIVDEVQGFFEVHRALGTHPGGIHVEITGEN
                     VTECLGGAQDISDTDLAGRYETACDPRLNTQQSLELAFLVAEMLRD"
BASE COUNT         1091 a         2519 c         2401 g         1223 t
ORIGIN      
        1 cgagttgacc gagttgcaca tccatccccg cgcccagggc cgcgggctcg gcgaggcgct
       61 ggcccgccgg ctgctcgccg gccgcgccga ggagaacgtc ctgctgtcca cgccggaaat
      121 caacggagag cccaaccgcg cgtggcgttt gtaccgccgg ctgggcttca ccgacatcat
      181 ccgcggctac cgcttcaccg gcgacccccg ggcattcgcg atcctgggcc gcgccctccc
      241 gctttagcgg cccggtagcg acctggtcgc ggccccgcag cagcccacgc cagcacgcac
      301 gacgcgccga cagcgcgaga ctggatgacc cgccgaagcg gcacacccgg atctggcacg
      361 atgacctggt gcgcgccagc cgtcccccgc tcctgacccg acgatcccga ggatttactg
      421 cccggcggcg ccgcgtgctg gccatcgcga tgctgctcct gctggtgccc ctggccaccg
      481 cgtgcctgcg ggtcagggcg tcgatcacgg tctcgccgga tgacctggtg tccggagaga
      541 tcatcgcggc ggccaagccg aagacgccca aggacaccgg tccgcagctg gacaccaata
      601 acctgccctt cagccagaag gtggcggtgt cgaactacga cagcgacggc tacgtgggtt
      661 cgcaggcagt gttctccgac gtgaccttcg ccgagttgcc gcagctggcg aacatgaact
      721 ccaacgccgc cggcgtgaac ctgtcgctgc gccgcaacgg caatctggtc atcctggagg
      781 ggcgggcaga cctgacatcg gtgaccgact ccgacgccga cgtcgagttg accgtcgcct
      841 tccccggcgt ggtgacctcc accaacggtg accgcgtcga gtccgacgtg gtgtcgtgga
      901 agctgaaacc cggcgtggtg agcacgatga ccgccaacgc ccgctacacc gaccccaaca
      961 cccgtaactt caccggggcg ggcatctggc tcggcgtcgc gtccttcgcg gttgccggcg
     1021 tggtggcgct gctggcgtgg atgagccgcg accgctcccc gcggttcacc gccgggggcg
     1081 accaaccgcc cagctcgggc taaacctccg gcgcgggtga ccagtaggcc agcatctccg
     1141 cgaacgtctg gaacgcgggc ccggaattcc cgtacgtcgc ctcgagatgg atgctcagcg
     1201 ggaaaccgag ctcggcgacc ccgtcgatca cctgcttgta caggtcgacc atgaggcggc
     1261 gcttctcggc cggctcgccg ccggccagcc tcttgacgaa cgcttgctcg tcggccaccg
     1321 cggcgttgcc cggatcctga atcagccagt tgatcaggcc gatccggctc tcgaccttcg
     1381 gcacgaagcc gaacgacagc aggatttcgg gccggtgatc agtgttcttg gcgaactcgg
     1441 tcaggaaacc cacgatcgcg tcggaataca acagctgtgt catcccgtag gtcgcgccct
     1501 gatcgcattt gaaattcagc cggccttgtt cgctgtcgcg ggtcgggatc acaatcacgc
     1561 cgcggttggc cacctgctcg cggtagatcg ataaggcgtc ggtcggcgcg acaccggaac
     1621 cttcgccgtc gttcatcgtg cgcggcaccc cgacgaagac cacgccctcc attccggcgg
     1681 cggacagatc gctgagccga cggcccagcg tcgcgtcgtc catgaacgcg gtcacctgcg
     1741 tgcacagtcc gcggaacccg ggcagctccg gtttgatgat cgaccagaag tccagcacgt
     1801 ccagtttcgg ttccatcgcc acgggacgat cgtcttcctc ggcgataatt ccggggatca
     1861 tcgcgtgccg gatccgaccc tcgaggccga agtcggccga gtggcgcacc actttccgcg
     1921 cgtcttcgag tgcccgctcc cgaccgtctg cgacgttcgg cggcaccaac tcgagcgcga
     1981 tggtgttcag ggtcacgcgg ctcctctccc tctctcgact ccatcacacg actttgccca
     2041 caccgttgcc cggcggcggc taagccgcgc ggcgcccgac accccgccag cataggcgtg
     2101 ggggtgccgc ccgcttgcgg ggcaatggtg aggcggtagc agcgagcggg accgccgaaa
     2161 cgacatacac tgtcggccct agaaccaata cacatcacgc cgagcagaaa ggggagccgc
     2221 gctgagccta aaatccgcag aagcagcagc aaccctcgag ttgaccggcg cgatcaccga
     2281 gcaacttcgg cggtatctgc aggaccgccg cgccgagacc gcctacatcg gaggcgatta
     2341 cgagaccctg accgccgccc tcgaagactt cgtgctcaac gggggcaagc ggctgcgacc
     2401 cgcgttcgct tattggggct ggcgcgcggt ggcacccgcg gatcccgatc ccgaagccct
     2461 gcggctattt tccgcgctgg agttgctgca cgcctgcgcg ctggtgcatg acgacgtgct
     2521 cgacgaatcg tccacccgcc gcggcaggcc gacgactcac gtccacttcg cggcgctgca
     2581 ccgcgaccgt ggctggcggg gctcaccgga tcggtttggc acgtcggcag cgatcctgct
     2641 cggcgacctg gcgttggcct gggccgacga catcgtcttt gcgctggagc tgccccccga
     2701 ggccctgcgg cgcgtgcacc gcgtgtgggc cgacatccgc accgaggtgc tcggcgggca
     2761 gtacctcgac atcgtcgccg aggccagcgc cgccgagtcc gtcgcgtcgg cgatgaacgt
     2821 cgacatctac aagaccgcct gttacacggt ggcgcggccg ctgcaactcg gggtggccgc
     2881 cgccgcggac agacccgatg tgcaagccgt cttcgggcaa ttcgggaccg acgtcggcgt
     2941 ggcgttccag ctgcgtgacg acgtgctggg cgtgttcggg gatccggcgg tgaccggcaa
     3001 gccgtccggt gacgacttga gatcgggcaa acggaccgtg ctgctggccg aggccgtgga
     3061 gctggcaaca aggtcagatc cggtggcggc caacctgtta cgcagctcga tcggcgcgca
     3121 cttgaccgac gcacaggtgg cccaactgcg agatgtcatc gagtcggtgg gcgcgctggc
     3181 ggcggccgag cagcgcatcg ccgcgttgac ccgacgggcg ctggccacgc tcgcggccgc
     3241 tcccatcgac gcggcggcca aagcggggct ctccgaactg gtccggatgg ctacgaatcg
     3301 gtccgcctga gccgatgacg acgcccgctg attcgagtat gcacagcccg gccgctgact
     3361 cgcagccgac gaaattctct gttgctcaac acttttcgcg tctacgtgcg ttcgccacca
     3421 ccgccgaggc ccgaccggcg aagcttggct tgctgggctc ggtactgatc accctgggcg
     3481 gcctgggagc gggcagcacc aagccgcacg acccgctgct cgaagcgttg cacctgtcgt
     3541 ggctgcgttt cggccacgga ctggtgctct cgtcgatcgt gttgtggacg ggcgtggggc
     3601 tcatgctgat cgcgtgggtg caactgggcc gacacacgct ggccgggaag acatccgagt
     3661 tcaccatgcg ggccaccacc ggtttctggc tggcgccgct gctgctgtcg gtgccggtct
     3721 tcagtcgcga tacctactcg tatctggccc agggcgcgct cctgcgcgac ggcttcgacc
     3781 cctatgcggt gggcccgata gccaatccga atgtgttgct ggacaacgta agtcccatct
     3841 ggaccatcac cacggcgccc tacggcccgg cgttcatcct ggtcgccaag ctcgtgacga
     3901 tgatcgtcgg caatcacgtg atcgccggga ccatgttgct gcgcctgtgc atgctgcccg
     3961 gactggcact gctgatgtgg gccgcccccc ggatggccca ccatcttggt accagcggcg
     4021 cgctggcgct gtggatctgc gtgctcaacc cactggtgct catccacctg atgggcgggg
     4081 tacacaacga gatgctgatg gtgggcctga tggccgccgg gatcgccctg accttccaag
     4141 gccggcacgt gctgggcgtc gtcttgatca cggtcgcgat cgcggtcaag gccaccgccg
     4201 gaatctcgtt gccgttcctg gtctgggtgt ggatgaggca tctgcgcgac caacgcggat
     4261 atcgccccgc gcgggcgttc ctggtggcca ccacgacctc cgcgctgatc ttcgccgtcg
     4321 tgttcgggat tctgtccgcc gtggccggtg tcggtctggg gtggctgacc gcattggccg
     4381 gatcggtgaa aatcatcaac tggctcagcg tgccgaccgc ggtcgccaac ctcatccacg
     4441 ccctgtgcgg caggttcttc ccggtggact tctacgccac gctgcgagtc acccggttga
     4501 tcggcgtggt gatcatcgcg gtgtcattgc cgatcctgtg gtggcggttc cggcgtgacg
     4561 accgcgccgc actgaccgga atcgcgtggt cgatgctggt ggtggtgctg ttcgtgcccg
     4621 ccgccctgcc gtggtactac tcctggccgc tggcggtggc ggccccgctg gcccagtcac
     4681 gacgggcggt cgcggccatc gccggcctgt cgacctgggt gatggtgatc ttcaaacccg
     4741 atggctcgca cggcatgtac tcgtggctgc acgtttcgct ggccaccgcg tgcgcgctgg
     4801 ccgcctggta tgcgctgcac cgctcgccgg agccggtggc tgaccgggac cgaaagcccg
     4861 tcgccgcgta gtcaggccgt cagtaggcca tggcttgcgc ccggcgcaca acctcgcggg
     4921 cctggtgggc gtgcagggcg tcgacgggac gggcgttgct gatggcgtcg cgggtgccgt
     4981 cgcgcgtgac cgtcagcgac gggtcggggg tgaacaacca gcggacgatc tcggtctcgt
     5041 ggtaaccgcc gtcgtgcagg atcgtcagca gccccggcag gctcttgacc acctcaccgg
     5101 agttggtgaa gaacacttgc ggtaccacca ccccgccgcc gcggcgaacc gcgagcaggt
     5161 ggccttcccg cagctgctgg tgcaccttgc tgaccgccac gccgagcaac tgcgcgacgc
     5221 gggtcaggtc gtaggtgggc tcctcaggat ccaagacgtc gtcgccggcc ggaatactgc
     5281 tcaccgcgcc agtgtaggcc cccgatcgcc cggttgagcc cgtgtttgac gccctacacc
     5341 tacgatggcc cagtggccgg agctgggacg ggagacccat tggagggcac gttgctggat
     5401 ggccgctacc tcgttcagtc caagatcggc agcggcggca cctcgacggt gtaccgcggc
     5461 ctcgacgtcc ggctggaccg cccagttgcg ttgaaggtca tggactctcg ctacgccggc
     5521 gatgatcaat tcctgacccg cttccagctc gaggcccgta gtgtcgcccg gttgaaaaac
     5581 cccggcctgc tcgccgtcta tgaccagggc ctggatgccc ggcacccgtt tctggtgatg
     5641 gagctcgttg agggcggaac gctgcgcgag ctcttgaccg agcgcggtcc gatgccgccc
     5701 cacgcggtgg tggcggtgct tcgtccggtg ctgggcgggc tgggcgccgc acatcgggcg
     5761 ggcctggtgc atcgcgacgt caagccggag aacgtcctga tctccgatga cggcgaggtc
     5821 aaaatcgccg atttcgggtt ggtccgcgcc gtggcggccg ctggaatcac ctccgccagc
     5881 gtcattctgg gcaccgccgc ctatctgtcg cccgagcaag tgcgcgacgg caacgccggt
     5941 ccgcgcagcg acgtctactc gaccgggatc ctcacctatg agctgctgac cgggcgcaca
     6001 ccgttcaccg gcgactcgac attgtcgatc gcctaccaac ggctggacac cgatgtgccg
     6061 ccgcccggcg cggcgatcga cggcgtgcca ccgcaattcg acgaattcgt gcggcgggcg
     6121 acgacgcgcg actcggccgt ccgatacgcc gacgcgctcg agatgggcgc cgaactggac
     6181 gcgatcgccg acgaactcgc cctgcccgac ttccgggtgc cggcgccgcg taactcggcc
     6241 caacaccgat cggcggcact gcaccacagc cggatgagcc agcgcccgcc cgtcgcggca
     6301 ccggttcccc accccacccg tcagctgacc cgcgggcccg gggattggtc cgaaccccgg
     6361 cgcccggcgg acctcgacgc tgaacccgac gactacgaat acgaggcgat ctcaggcgaa
     6421 ttcgcgggca tcgcgatgag cgagttcgtc tgggcccgtc agcacgcccg ccggatggtg
     6481 ttggtctggg tggcggtcgt attggcggcc accggactgg tggcgaccgc ggcgtggacg
     6541 atcggcagca atttgcgcgg actgctctag cttcgtcgac gcaccgacat gcacgctcgg
     6601 tgcctgcgcc tgctagtcgc gcagcatttc ggcgaccagg aacgccaact ccagcgactg
     6661 ctgggtgttc agccgcgggt cgcacgccgt ctcgtagcgg ccggccaggt ccgtgtccga
     6721 aatgtcttgc gcgccaccca agcactcggt gacgttctcg ccggtgatct ccacatggat
     6781 gccaccggga tgggtaccca gcgcccggtg cacctcgaag aatccctgca cttcgtcgac
     6841 gatgcggtcg aagtgccggg tcttgtagcc gttggacgac tcgtgggtgt tgccgtgcat
     6901 cggatcgcac tgccagatca cctggtgccc ggtggcctgg accttctcga cgatcggcgg
     6961 cagcaggtcg cgcaccttgt tgttgcccag cctcgtcacc aacgtcagcc ggcccggctt
     7021 gttgtgcgga tcgaggcgct cgacgtattc gacggccact tccggggtca tcgtcgggcc
     7081 gatcttgacc ccgatcgggt tggcgatcac ctccgcgaac gcgacatggg cgccgtccag
     7141 ctggcgggta cgctcgccga tccacacagt gtgcgccgac aggtcataca gctgctgctc
     7201 cccgtcctcg gcgtccgaga ggcgcaacat ggcg
//
LOCUS       DASSGQ010000369         3654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_28130, whole
            genome shotgun sequence.
ACCESSION   DASSGQ010000369 DASSGQ010000000
VERSION     DASSGQ010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28130"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="VFI55_16325"
     CDS             <1..1320
                     /locus_tag="VFI55_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014350509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7075631.1"
                     /translation="EVGGGTRSSEAIVPGLLHDNCSAIHPMAVGSQFFKDFNLDRYGL
                     AWRWPEIDCVHPLDDGNAGVLYRSVEQTAAGLGRDGSRWQRAFGYSAARFDALSEDIM
                     GPLLRIPHHPLMLARFGAPTVLPASTFARLFRTEEGRALFGGVAAHAFRPLHYPMTSA
                     IGLGIIAAGHRHGWAVAEGGSQSIANAMVALLSDLGGKIETGVRVQTATQLPPADVTL
                     FDLAPSGVAGILGDRLPRRISRAFNRFRRGPGAFKVDFAVEGGVPWTNPHAHLAGTVH
                     LVGNYSELAATEREIHAGRMPERPFVLIGQQYLADPQRSVGNVHPVWSYAHVPNGYAG
                     DATEAIIAQIERFAPGFRERVVGFAVRSTTQMAAYNLNYDGGDILTGAKDIRQMTFGP
                     RVTLSPYTIGVPGMYICSAATPPGPGAHGMCGANAAKLALAYLARRA"
     gene            complement(1348..2223)
                     /locus_tag="VFI55_16330"
     CDS             complement(1348..2223)
                     /locus_tag="VFI55_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HET7075632.1"
                     /translation="MTGSYEYTFEDIQNRPVAVVGAGTLGRRVAVMFASRGGTVRIFA
                     RRHEQLAAATQYVTETLPKVVADRGFGEVGGATAAGSLEEALDDAWLVVESVPEKLEI
                     KIPLWGLIDQLAPPDTIFATNSSSYPSRLMAENVRDKRRLCNMHFYMPPEINAVDLMS
                     DGETDRGLLDMLLRVLPEFGVYPFEALKECTGFIFNRIWAAIKRESVAVVAEGVARPE
                     DVDGMFRVNWGVPAGPFQMMDLVGLDVVLDIENHYAEEFPYLPKGVRDLLQSYVDAGK
                     LGVKTGEGFYTYPKP"
     gene            2436..>3654
                     /locus_tag="VFI55_16335"
     CDS             2436..>3654
                     /locus_tag="VFI55_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016882611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7075633.1"
                     /translation="MADVDQHASHRDRVPDIPAELRDAGFENAAEIGRGGFGVVYRCD
                     QPMLDRVVAVKVLIADLDLENLDRFLREQHAMGRLSGHPNIVTILQVGTTTSGRPFIV
                     MPFHAKNSLEALIRRHGPLDWRETLRIGVKLAGALEAAHLAGILHRDVKPGNIMLSEY
                     GEPELTDFGIARMAGGFETATGVITGSPAFTAPEVLEGKTPTPASDVYSLGATLFCAL
                     TGHAAFERRTGEKVIAQFIRITSQPIPDLRESGFPPEVAAVIERGMEREPGARPASAA
                     AFGEELREVQRTGGVAVDEMAHPVELGVERRTSPVTASQARRDTPTTMKATLTPPTPA
                     TKYRPPVATRSLVARERLIEVLRAGDRRRFVLIHAPSGFGKSTLAVQWRDELTRDGVA
                     VGWLTIDDDDNNVV"
BASE COUNT          591 a         1222 c         1233 g          608 t
ORIGIN      
        1 gaggtcggcg gcggcacccg cagcagcgag gccatcgttc cgggcctgct gcacgacaac
       61 tgctcggcga tccaccccat ggccgtcggc tcccagttct tcaaagactt caacctggac
      121 cggtacggcc tggcgtggcg gtggccggag atcgattgtg tccatcccct cgacgacggc
      181 aacgccggcg tgctgtaccg ctccgtggag cagaccgcgg ccggtctcgg tcgcgacgga
      241 tcgcggtggc aacgcgcgtt cggctactcg gccgcccggt tcgacgcgct cagcgaggac
      301 atcatgggac cgctgctgcg gatcccgcac cacccgctga tgctggcccg cttcggtgcg
      361 cccaccgtcc ttcccgcgtc cacgttcgcg cggctgttcc gcaccgagga ggggcgcgcc
      421 ctgttcggag gcgttgcggc ccatgccttc cggccgctgc actaccccat gacgtcggcc
      481 atcgggttgg gcatcatcgc cgccggccac cggcacggct gggcggtggc cgagggcggg
      541 tcgcagtcga tcgccaacgc gatggtggca ttgctgagcg atttgggcgg caagatcgag
      601 accggcgtcc gggtgcagac ggcgacgcag ctgccacccg cggacgtcac gctgttcgat
      661 ctggcgccgt ctggcgtcgc cgggatcctc ggggaccgtc tcccacgccg aatttcgcgt
      721 gccttcaaca ggtttcggcg cgggccgggt gcgttcaagg tcgacttcgc cgtcgagggc
      781 ggcgtgccct ggacgaatcc gcatgcccac ctggccggca cggtgcacct ggtggggaac
      841 tactccgaac tcgccgcgac cgagcgcgag atccacgccg gccgcatgcc cgagcgcccg
      901 ttcgtgctga tcgggcagca gtatctggcc gatccgcaac gctcggtggg caacgtgcat
      961 ccggtgtgga gctacgcgca cgtcccgaac ggctacgccg gtgacgcgac ggaggcgatc
     1021 atcgcccaga tcgagcggtt cgccccgggt ttccgggagc gcgtcgtggg gttcgcggtg
     1081 cgatcgacga cgcagatggc cgcatacaac ctcaactacg acggcggcga catcctgacc
     1141 ggcgccaagg acattcgtca gatgacgttc gggcctcgag tcacgctgtc gccgtacacc
     1201 attggagtgc ccggcatgta catctgctcg gcggcgaccc cgccggggcc cggcgcccac
     1261 ggcatgtgcg gcgccaacgc cgcgaagctc gcgctggcgt acctggctcg gcgagcgtag
     1321 cgcgacagcg gttcagggtt tgggatttca gggtttggga taggtgtaga agccctcgcc
     1381 ggtcttgacg ccgagcttgc cggcatcgac gtaggactgc aacaagtccc gcacgccctt
     1441 tggcagatac gggaactctt cggcgtagtg gttctcgatg tcgagaacga cgtcgagtcc
     1501 aaccaggtcc atcatctgga atggcccggc cggcacgccc cagttcaccc tgaacatgcc
     1561 gtcgacgtcc tccggacggg ccacgccctc ggcaaccacg gccaccgact cgcgcttgat
     1621 ggccgcccag atccgattga agatgaaccc agtgcactcc ttgagcgcct cgaagggata
     1681 gacaccgaac tcgggcagca ccctcagcag catgtcgagc aggccgcggt cagtctcgcc
     1741 gtccgacatc agatcgacgg cgttgatttc cggtggcata tagaagtgca tgttgcacaa
     1801 ccgccgcttg tcgcgcacgt tttcggccat caggcgagac gggtatgacg atgagttggt
     1861 cgcgaaaatg gtgtccggcg gcgccagttg gtcaatcagg ccccacagcg ggatcttgat
     1921 ttcgagcttc tccgggacgg actcgacgac aagccaggcg tcgtccaacg cctcttcgag
     1981 tgaccccgcg gccgtcgcgc cgccgacctc gccaaagccc cggtcggcca ccaccttcgg
     2041 cagcgtctcc gtcacgtact gcgtggcggc ggcgagctgt tcgtggcgcc gcgcgaatat
     2101 tcgcacggtt ccgccgcggc tggcgaacat caccgctacg cggcgaccca gtgttcccgc
     2161 accgaccacc gccaccgggc ggttctggat gtcttcgaat gtgtattcgt acgagccagt
     2221 cacacacgca tgctagatcc cggaagcgag cctgcaacca tcgccaggcg acaaagatgg
     2281 ccgcagtgtt gtccgcggtg gacgacgcgg gcagcgagcg gcctgatcta ctcgggccgg
     2341 tgcccgcacg ccgccccact ctcagcccac cctcgcgact tttttgtagc cggaaacaga
     2401 ggtcgacggt cgcccgcgtg ggatagtggc gattaatggc cgacgtcgat caacatgcgt
     2461 cgcatcggga tcgggtccct gacatacccg cggaactgcg cgacgcgggc ttcgagaatg
     2521 ccgccgaaat tgggcggggc ggcttcggtg tggtgtatcg ctgtgatcag ccgatgctgg
     2581 accgcgtggt cgccgtcaag gtgttgatag ctgacctcga cctggagaac ctcgaccgct
     2641 tcctgcgcga gcagcacgcg atgggtcggt tgtccggcca ccccaacatc gtgacgatat
     2701 tgcaggtcgg caccaccacc tctggtcggc cgttcatcgt gatgcccttc cacgcaaaga
     2761 attcactgga ggcgctgatc cgtcggcacg ggccgctgga ttggcgtgag acgctgcgca
     2821 tcggcgtcaa gctggcggga gcgctcgaag ccgcccatct ggcgggcatc ctgcatcgcg
     2881 acgtcaagcc gggcaacatc atgctgagcg agtacggcga accggagctg accgacttcg
     2941 gcatcgccag gatggccggc ggcttcgaga cggcgaccgg tgtgatcacc ggttcgccgg
     3001 cgtttaccgc acccgaagtg ctcgagggca agacgcccac acccgcatcg gacgtgtatt
     3061 cgcttggcgc gacgctgttt tgcgcactga ccggccacgc cgcgttcgag cgtcgcaccg
     3121 gcgagaaggt gatcgctcag tttattcgca tcacgtcgca accgattccg gatctgcggg
     3181 agagtggttt cccgccggag gtggcggcgg tcatcgagcg gggcatggag cgcgaacccg
     3241 gggcgcgtcc ggcgagcgca gcggcgttcg gcgaggagct gcgcgaggtt cagcgcacgg
     3301 gcggtgtggc cgtcgacgag atggcccatc ccgtcgaatt gggggtggag cgccggacgt
     3361 ctccggtgac cgcgtcacag gcacggcggg atacccccac cacgatgaag gccacgctga
     3421 cgcccccgac tccggcgacg aaataccggc cgccggtggc gacccgatcg ctggttgcgc
     3481 gcgagcggct gatcgaggtg ttgcgggcgg gcgatcggcg acggttcgtc ctcatccacg
     3541 ccccttcggg cttcggcaag agcacactgg cggtccagtg gcgagacgaa ctcacgcgcg
     3601 acggcgtcgc ggttggctgg ctgacgatcg acgacgacga caacaacgtg gtgt
//
LOCUS       DASSGQ010000370        10454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1243057,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000370 DASSGQ010000000
VERSION     DASSGQ010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10454
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243057"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFI55_16340"
     CDS             <1..499
                     /locus_tag="VFI55_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075634.1"
                     /translation="GTPGSHWASVEPSSPPAGERSGRRRRSRHSGPEELLDGAPVEAG
                     ASQPPAESGRRARSRHSAEYRDYGVRNFAAMSESPPPAPVSAPAAAPPPLPANAPAAP
                     PPPPQMASPPAAEPVPRHGGADPSDESDSSEDGPPTGGQSVADLFARLHVEPSGGGRR
                     RRRDG"
     gene            616..1524
                     /locus_tag="VFI55_16345"
     CDS             616..1524
                     /locus_tag="VFI55_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HET7075635.1"
                     /translation="MVQFDGLRPARLKVGIISAGRVGSALGVALERADHVVVACSAVS
                     HASRQRAQRRLPDTPVLSPPDVAANAELLLLAVPDSELGGLVSGLAATSTVRPGTIVV
                     HTSGANGVDILAPLAPRCVPLAIHPAMTFTGSDEDISRLPDTCFGITAADEVGYAIGQ
                     SLVLEMGGEPFCVREDARVLYHAALAHAGNHVVTVLADALEALRAALRGSELLGQQAV
                     DEQPGGLAERIVGPLARAALENTLQRGQAALTGPVARGDAVAVAEHLAALADVGPELA
                     QAYRVNALRTAQRAHAPDDVVEVLAP"
     gene            1521..2468
                     /gene="panC"
                     /locus_tag="VFI55_16350"
     CDS             1521..2468
                     /gene="panC"
                     /locus_tag="VFI55_16350"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET7075636.1"
                     /translation="MKPAKPPAFKPGQLNVYSTPRDVTDVSRALRHTGRRVMLVPTMG
                     ALHHGHIALVRAAKRVPGSVVVVSIFVNPLQFGAGEDLDAYPRVLDDDLALLRAEDVE
                     IVFAPTAAAMYPNGLRTTVHAGPLAAELEGGPRPSHFTGVLTVVCKLLQIVCPDRVFF
                     GEKDYQQLVLVRQMVADLNIDVAVVGVPTVREVDGLAMSSRNRFLDPVQRDLAGALSA
                     ALTAAAHAAPGGAAAALNAARAVLDAVPAISVDYLELRDADLGPVRSDAPGRLLVAAR
                     LGTTRLLDNIAIHIGADKTAPAPVGPDGYRDTVESRWRN"
     gene            2468..2899
                     /gene="panD"
                     /locus_tag="VFI55_16355"
     CDS             2468..2899
                     /gene="panD"
                     /locus_tag="VFI55_16355"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HET7075637.1"
                     /translation="MLRTMLKSKIHRATVTQADLHYVGSVTIDADLMDAADLLEGEQV
                     TIVDIDNGARLVTYAITGERGTGVIGINGAAAHLVHPGDLVILLAYGTMEDAEARAYE
                     PRILFVDAANKQIDLGHDPAFVPDLEIPGTAELLDPRIGAR"
     gene            2902..3726
                     /locus_tag="VFI55_16360"
     CDS             2902..3726
                     /locus_tag="VFI55_16360"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET7075638.1"
                     /translation="MLLAIDVRNTHTVVGLLSGSKEHAKVVQQWRIRTESEATADELA
                     LTIDGLIGDDSERLTGAAALSTVPSVLHEVRIMLDQYWPSVPHVLIEPGVRTGIPLLV
                     DNPKEVGADRIVNCLAAFHKFGKAAIVVDFGSSICVDVVSAKGEFLGGAIAPGVQVSS
                     DAAAARSAALRRVEMVRPRSVVGKNTVECMQAGAVFGFAGLVDGLVTRIREDVDGFSA
                     DLGDQVATVATGHTAPLLLPELHTVNHYDQHLTLHGLQLVFERNLDTQRGRLKTAR"
     gene            3848..5368
                     /gene="lysS"
                     /locus_tag="VFI55_16365"
     CDS             3848..5368
                     /gene="lysS"
                     /locus_tag="VFI55_16365"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET7075639.1"
                     /translation="MSAASDTGADVPEQFRIRRDKRARLLAEGRDPYPVAVVRTHTLA
                     EVRAAHPDLPIDCETDDVVGVAGRVVFARNSGKLCFATLQDGDGTSLQVMISLDKVGR
                     EALDAWKADVDLGDIVYVHGNVISSRRGELSVLADSWQMASKSLRPLPVAHKEMSEEL
                     RVRQRYVDLIVRPQARAVARQRIGVIRAIRNSLERRGFLEVETPMLQTLAGGAAARPF
                     TTHSNALDIDLYLRIAPELFLKRCVVGGFDKVFELNRVFRNEGADSTHSPEFSMLETY
                     QTYGTYDDSAIVTRQLIQELADEVIGTRQLPLPDGSVYDIDGEWASIQMYPSLSAALG
                     EEITPGTTVDHLCAVAQRLGLEIGPQITGNRDYVHGKLVEELWEHTVGNTLSAPTFVK
                     DFPVGTTPLTRAHRSIPGVTEKWDLYLRGVELATGYSELNDPIVQRERFAEQARLAAA
                     GDEEAMVLDEDFLTALEYAMPPCTGTGMGIDRLLMSLTGLSIRETVLFPIVRPHSS"
     gene            5490..5828
                     /gene="lsr2"
                     /locus_tag="VFI55_16370"
     CDS             5490..5828
                     /gene="lsr2"
                     /locus_tag="VFI55_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein Lsr2"
                     /protein_id="HET7075640.1"
                     /translation="MAKKVTVTLVDDFDGAGAADETVEFGLDGVTYEIDLSSKNAAKL
                     RGDLKQWVAASRRVGGRRRGRSGSGRGRGAIDREQSAAIREWARRNGHNVSTRGRIPA
                     DVIDAFHAAT"
     gene            6109..8655
                     /gene="clpC1"
                     /locus_tag="VFI55_16375"
     CDS             6109..8655
                     /gene="clpC1"
                     /locus_tag="VFI55_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpC"
                     /protein_id="HET7075641.1"
                     /translation="MFERFTDRARRVVVLAQEEARMLNHNYIGTEHILLGLIHEGEGV
                     AAKSLESLGISLEGVRSQVEEIIGQGQQAPSGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLVKLGAELTRVRQQVIQLLSGYQGKEAAEAGTGGRGG
                     ESGSPSTSLVLDQFGRNLTAAAMEGKLDPVIGREKEIERVMQVLSRRTKNNPVLIGEP
                     GVGKTAVVEGLAQAIVHGQVPETLKDKQLYTLDLGSLVAGSRYRGDFEERLKKVLKEI
                     NTRGDIILFIDELHTLVGAGAAEGAIDAASILKPKLARGELQTIGATTLDEYRKYIEK
                     DAALERRFQPVQVGEPTVEHTIAILKGLRDRYEAHHRVSITDGAIAAAATLADRYIND
                     RFLPDKAIDLIDEAGARMRIRRMTAPPDLRDFDEKIAEARREKESAIDAQDFEKAASL
                     RDREKQLVGQRAEREKQWRSGDLDVVAEVDDEQIAEVLGNWTGIPVFKLTEAETTRLL
                     RMEDELHKRIIGQEDAVRAVSKAIRRTRAGLKDPKRPSGSFIFAGPSGVGKTELSKAL
                     ANFLFGDDDALIQIDMGEFHDRFTASRLFGAPPGYVGYEEGGQLTEKVRRKPFSVVLF
                     DEIEKAHQEIYNSLLQVLEDGRLTDGQGRTVDFKNTVLIFTSNLGTSDISKPVGLGFT
                     QGGGDNNYERMKQKVNDELKKHFRPEFLNRIDEVIVFHQLTRDEIIRMVDLMIGRVAG
                     QLKSKDMELVLTDKAKSLLAKRGFDPVLGARPLRRTIQREIEDQLSEKILFEEVGPGQ
                     VVTVDVDNWDGEGAGEDVVFTFTGTRKPPTEPDLAKAGAHTAGGPGPSAQ"
     gene            8737..9117
                     /locus_tag="VFI55_16380"
     CDS             8737..9117
                     /locus_tag="VFI55_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HET7075642.1"
                     /translation="MLIVTTNDLPGWEIQRVCGEVFGLTVRSRNAFAQFGAGLKSMFG
                     GELAGMTKNLAESRNEAMGRLIAEARNRGGNAIVAMRFDTTEIGDVWTEICAYGTAVQ
                     AVPVTDAARYTANQLGYGGPAPQH"
     gene            9263..9478
                     /locus_tag="VFI55_16385"
     CDS             9263..9478
                     /locus_tag="VFI55_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtB-domain containing protein"
                     /protein_id="HET7075643.1"
                     /translation="MSNPQATGARVRSVDLSATSATLWLTATAFLALLAIYFVGVDQG
                     AVSLFGSDMHVHEFVHDARHLLGFPCH"
     gene            9500..10291
                     /locus_tag="VFI55_16390"
     CDS             9500..10291
                     /locus_tag="VFI55_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HET7075644.1"
                     /translation="MEKRLIARGLLAGFAGALLAFLFARLCAEPVIGRAVAFEDGRTD
                     AENAHGVHEHGVDLFSRGVQSNVGLGFGVLIFGVAMGALFAVLFCVVYSRVRSLEPRV
                     LSLLLAAGAFVAVYLVPFVKYPPNPPAVGQPDTIRARTGWYLVMVLASVVLAIAAVWL
                     ARRLAARFGAWDGRLLASASYVVAVAVVMVLLPTVDETPQPLRDASGAIIYPGFPADV
                     LYEFRLLSLGTQLVLWTTIGLVFATLAGRVLGERAASGRAPSIAA"
     gene            10288..>10454
                     /locus_tag="VFI55_16395"
     CDS             10288..>10454
                     /locus_tag="VFI55_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7075645.1"
                     /translation="MTEVVRLTLVSHAMTDAMAAGRFPGDEPLNGVGRRQVEAVAAQI
                     NRDVRQLTGPER"
BASE COUNT         1783 a         3352 c         3576 g         1743 t
ORIGIN      
        1 gggaacgccg ggcagccact gggccagtgt cgagccgtcc agtccaccgg ccggcgaacg
       61 gtcgggccgg cgccgccggt cccggcattc cggccccgag gaattgctcg acggcgcgcc
      121 cgtcgaagcc ggggcctcgc agccacccgc cgagtccggt cgccgggcgc ggtcgcggca
      181 ttcggcggag taccgggact acggcgtgcg caacttcgcg gccatgagcg agtccccgcc
      241 gcctgcgccc gtgagtgcgc cggctgccgc gccgccgccc cttcccgcga atgcgccggc
      301 cgcgccgccg ccgccaccgc aaatggcctc gccgccggcc gcggagcccg tgccccggca
      361 cggcggcgcc gacccttcag acgagtcgga cagctccgag gacggcccgc caaccggtgg
      421 tcagtcggtt gccgatctct tcgcgcggct gcacgtcgag ccgtctggag gcggccggcg
      481 tcgtcgccgc gacgggtgaa tcggcagccc ggtcgcattg gtgccgcctg acgaatcgac
      541 tacagtctta gtgaccgtcc ggtacccgca gcacgggact ggaacgaacg aaagacacag
      601 gtgaggtcgt ctgcgatggt gcagttcgac ggtttgcgcc cggccaggct caaggtgggg
      661 atcatctcgg ccggccgggt aggttccgcg ctgggtgtcg cgctggaacg cgccgaccac
      721 gtcgtggtgg cttgtagtgc cgtctcccat gcgtcgcggc agcgggcgca gcgccggctg
      781 cccgataccc cggtgctctc accaccggat gtggccgcca acgccgaatt gctgctgctg
      841 gcggttcccg acagcgaact gggcggcctg gtgtccgggc tggccgcgac ctcgacggtg
      901 cgacccggca cgatcgtggt gcacacgtcc ggcgccaacg gggtcgacat cctcgcaccg
      961 ctggcgccac gctgcgtacc gctggccatt catccggcga tgacgttcac cggctccgac
     1021 gaggacatca gccggctgcc cgacacctgc ttcgggatca ccgcggccga tgaggtcggg
     1081 tacgcgatcg ggcagtcgct ggtcctcgag atgggcgggg agccgttctg cgtccgcgag
     1141 gacgcccgcg ttctctacca cgccgcgttg gcgcacgcgg gcaatcacgt cgtgacggtc
     1201 ctggccgacg cgctggaggc gttgcgggca gcactgcgcg gcagcgaact gctcgggcaa
     1261 caagctgtgg acgaacagcc gggtggcctt gccgaacgca tcgtcgggcc gttggcccgg
     1321 gcggcgctgg agaacacgct gcagcgcggt caggcggcgc tgaccgggcc ggtcgcccgc
     1381 ggtgacgcgg tggcggtcgc cgagcatttg gccgcgctgg ctgacgtcgg gccggaactg
     1441 gcgcaggcct accgggtgaa cgccctgcgg accgcccagc gcgcgcacgc ccccgacgac
     1501 gtcgtcgagg ttctggcgcc ttgaaaccag ctaagccgcc cgccttcaag ccgggccaac
     1561 tcaacgtcta ctcgacgccg cgcgatgtca ccgacgtcag ccgtgccctg cgccacaccg
     1621 gccgccgggt gatgttggtg cccaccatgg gcgcgctgca tcacggacac atagccctgg
     1681 tgcgggcggc caagcgggtc ccggggtcgg tggtcgtggt ctcgatcttc gtcaacccgc
     1741 tgcaattcgg cgccggcgaa gacctcgacg cctatccccg ggttctcgac gacgatttgg
     1801 cgctgctgcg cgccgaagac gtcgagatcg tgttcgcgcc caccgccgcg gccatgtatc
     1861 cgaacggtct gcgcaccacc gtgcatgccg gcccgctggc cgccgaactc gagggtggcc
     1921 cccggccgag ccatttcacc ggcgtgctga cggtggtgtg caagctgttg cagatcgtgt
     1981 gtcccgaccg ggtgttcttc ggtgagaagg actaccagca gctggtcctg gtacgccaga
     2041 tggtcgccga cttgaacatc gacgtcgccg tggtcggcgt cccgacggtg cgcgaagtcg
     2101 acgggctggc gatgtcttcg cgcaaccgct ttctcgaccc ggtgcagcgc gacttggccg
     2161 gggcgctttc ggcggctctg acggcggcgg cgcatgcggc gccgggcggc gcggcggcgg
     2221 cgctgaacgc cgcccgcgcg gtgctggacg ccgttcccgc gatcagtgtc gactacctgg
     2281 agctgcgcga tgcggatctg ggcccggtac gcagcgacgc acccggccgg ctgctggttg
     2341 ccgcccggct gggcactacc cgactgctgg acaacatcgc cattcacatc ggagcagaca
     2401 aaactgcgcc ggcaccggta gggccggacg gatatcggga caccgttgaa tcacgttgga
     2461 ggaattgatg ttacggacca tgctcaagtc gaagatccac cgcgccaccg tcacgcaggc
     2521 tgacctgcac tacgtcggct cggtgaccat cgacgccgac ctgatggacg ccgccgacct
     2581 gctggagggc gaacaggtga ccatcgtcga catcgacaac ggtgcccgac tcgtcaccta
     2641 tgcgatcacc ggcgagcgcg ggaccggggt gatcggaatc aacggtgccg cagcgcatct
     2701 ggttcacccc ggggatctgg tgatcttgct ggcgtacggg acgatggaag acgcggaggc
     2761 gcgcgcctac gagccgcgga tccttttcgt cgacgccgcc aacaagcaga tcgacctggg
     2821 acacgatccg gctttcgtgc cggatttgga gattccgggg acagccgagc tgttagaccc
     2881 ccggatcggt gcgcggtagc ggtgctgctg gccatcgacg tccggaacac gcacaccgtc
     2941 gtcggactgc tgtcgggctc caaggagcac gcgaaagtcg tgcagcaatg gcggattcgt
     3001 accgaatccg aggccaccgc cgacgaactg gcgctgacca tcgacggcct gatcggcgac
     3061 gactccgagc gcctcacggg cgcggccgcg ctctcgacgg tgccgtcggt gctgcacgag
     3121 gtgcggatca tgctggatca gtactggccg tcggtaccgc acgtgctgat cgagccgggg
     3181 gtgcgcaccg gaatcccgct gctggtcgac aatcccaagg aagtgggtgc cgaccggatc
     3241 gtcaactgcc tggcggcgtt tcacaagttc ggtaaggcgg ccatcgtcgt cgatttcgga
     3301 tcctcgatct gcgtggacgt ggtgtcggcc aagggcgagt tcctgggcgg cgccatcgcc
     3361 cccggcgtgc aggtctcgtc ggatgccgcc gcggcccgat ccgcggcgtt gcgtcgcgtc
     3421 gaaatggtgc gtccccgttc ggtggtcggc aagaacaccg tcgaatgcat gcaggccggt
     3481 gcggtgttcg gcttcgccgg gctggtcgac ggattggtca cgcgcatccg cgaagacgtc
     3541 gacggcttct ccgccgacct gggcgatcag gtcgcgaccg tggccaccgg ccataccgcc
     3601 cccctgctgc tgcccgagct gcacaccgtc aaccactacg accagcatct gacgctgcat
     3661 ggcctgcagc tggtcttcga acgcaacctg gacacccagc gtggccggct gaagacggca
     3721 cgctgacggg gccgcaccac cgagtcgatt ctgcgtacag gaaccccctc gagcgcggat
     3781 tcgcgcgccc agcgcaggct cgacgccctc agcgcagact cgagtccgtc ctttaagctg
     3841 gcacgtcgtg agtgccgcct cagacacggg agcagacgtt ccagagcagt tccggatccg
     3901 ccgggacaag cgggcgcgcc tgttggcgga ggggcgtgac ccctatccgg ttgccgtcgt
     3961 gcgcacccac accctggccg aggtgcgcgc cgcccacccc gatctgccga tcgactgcga
     4021 gaccgacgac gtcgtcggcg tcgcgggccg ggtggtcttc gcacgcaact ccggaaaatt
     4081 gtgctttgcg acactccagg acggcgacgg caccagcctg caggtgatga tcagcctcga
     4141 caaggtcggt cgagaagcgc tggacgcatg gaaggccgac gtcgatctcg gcgacatcgt
     4201 ctacgtgcac ggcaacgtga tcagctcgcg acgcggcgag ttgtcggtcc tggccgactc
     4261 ctggcagatg gcgtcgaagt cgttgcggcc gctgcccgtc gcgcacaagg agatgagcga
     4321 agagttgcgg gtgcggcagc gctatgtcga cctgatcgtg cgtccgcagg cgcgcgcggt
     4381 ggcccggcag agaatcggcg ttatccgcgc gatacgcaac tcgctggaac gccgcggttt
     4441 tctcgaagtc gaaaccccta tgttgcagac gctggccggt ggtgccgcgg cccggccgtt
     4501 caccacacat tccaatgcgc tggacatcga tctctacctg cggatcgcgc cggaactgtt
     4561 cctgaagcgc tgcgtcgtcg gtggattcga caaggtcttc gaactaaatc gcgtgttccg
     4621 aaacgaagga gccgattcga ctcattctcc ggaattctcc atgctggaga cctaccagac
     4681 ctacggaacc tatgatgatt cggccatcgt cacgcgccag cttattcaag agctagccga
     4741 cgaggtgatc ggtaccagac aactgccgtt gcccgacggc agcgtgtacg acatagacgg
     4801 agaatgggct tccatacaaa tgtatccgtc gctgtcggcg gcgctcggcg aggagatcac
     4861 acccggcact acggtcgatc acctgtgtgc tgtcgcgcag agattgggat tggagatcgg
     4921 cccgcagatt accggcaacc gcgactacgt gcacggcaaa ctcgtcgagg aactgtggga
     4981 acacaccgtg ggcaacaccc tgtcggcgcc caccttcgtc aaggattttc cagtcggcac
     5041 aacacctttg acgcgcgcac accgcagcat cccgggcgtt accgagaagt gggacctcta
     5101 ccttcgtggc gtcgagctgg ccaccgggta ctccgaatta aacgatccga tcgtgcagcg
     5161 cgaaagattc gctgagcaag cccgactcgc ggccgcgggc gacgaagagg ctatggtgct
     5221 tgacgaagac tttctgacgg ctctcgagta tgcgatgcca ccctgcaccg gaaccggaat
     5281 gggtatcgat cggttgttga tgtctttgac cgggctgtca attagggaga cagttttgtt
     5341 cccgattgtt cgaccgcact ccagttgagt tcaactgtac gtcttgcgct cagattgagt
     5401 atgctgccct cttatggcag attggtgacc gggggaacaa tccaatctgc tccaagcaac
     5461 tgctcaggac gaaacgcgag ggtaagcaaa tggcgaaaaa agtgaccgtc accttggtcg
     5521 atgatttcga cggtgcgggc gcagccgacg aaacggtcga atttgggctt gacggggtga
     5581 cctatgagat tgatctttcg tctaagaatg ccgcgaaact ccggggagat ctgaagcaat
     5641 gggtggcggc cagccgtcgg gtgggcggtc gccggcgcgg tcgttccggt tcgggacgcg
     5701 gacgtggtgc gatcgaccgt gagcagagcg ccgcgatccg ggaatgggct cgtcgaaatg
     5761 ggcacaacgt gtcgacgcgg ggccgcatcc cggccgacgt gatcgacgca ttccacgcgg
     5821 cgacctaaca aaacgcttac cggcgcccgg gccccagggt ctgggcgccg gttgtttgtc
     5881 atttcgctcg cggcgaaatg attgaaggtc aacgcgggaa acggtttggt gtttttcctc
     5941 gtttatgtat tgcgcctgta gtcgcaactt ggggatgcgg gctggaagca ctgggcaccg
     6001 caaggttagg aaccctggca ggccgaccat tacagtggat ggcaggtatg caggagccga
     6061 cccgaggccg accaggtacc gatggtgagg gagagcaggt aaccaccgat gttcgaaaga
     6121 tttaccgacc gtgcccgcag ggtcgtcgtc ctggcgcaag aagaggccag gatgctcaac
     6181 cacaactaca tcggcaccga gcacatcttg ttgggcctga tccacgaggg cgaaggcgta
     6241 gccgccaagt ctctggagtc gctgggcatc tcgctggagg gcgtccggag ccaggtcgag
     6301 gagatcatcg gccaggggca gcaggccccg tcggggcata tcccgttcac cccgcgcgcc
     6361 aagaaggttc tcgagctgag cctgcgcgag gcgctgcagc tgggccacaa ctacatcggc
     6421 accgagcaca tcctgctggg cctgatccgc gagggtgagg gcgtggccgc gcaggtgctg
     6481 gtcaagctgg gggccgagct gacccgggtg cgccagcagg tcattcagct gctgagcggc
     6541 taccagggca aggaggccgc tgaggccggg accggcggcc gtggcggaga atcggggagc
     6601 ccgtcgactt cactggttct cgaccagttc gggcgcaacc tgacggccgc cgcgatggaa
     6661 ggcaagctcg acccggttat cggccgcgag aaggaaatcg agcgggtgat gcaggtgttg
     6721 agccggcgca ccaagaacaa cccggtgctg atcggcgagc ccggcgtcgg caagaccgcc
     6781 gtcgtcgagg gcctggcgca ggccatcgtg cacggccagg tccccgagac gctgaaggac
     6841 aagcagctct acaccctcga cttgggatcg ctggtggcgg gcagccgtta ccgcggtgac
     6901 ttcgaggaac gcctcaagaa ggtgctcaag gagatcaaca cccgcggcga catcatcctg
     6961 ttcatcgacg agctgcacac gctggtcggt gccggtgccg ccgagggcgc gatcgacgcc
     7021 gcgtcgatcc tgaagccgaa gctggcccgc ggtgagctgc agacgatcgg cgccaccacg
     7081 ctcgacgagt accgcaagta catcgagaag gacgccgctc tggagcgccg cttccagccg
     7141 gtgcaggtgg gggagccgac ggtggagcac accatcgcga tcctcaaagg tctacgcgac
     7201 cgctacgagg cccaccaccg ggtgtcgatc accgatggcg cgattgctgc cgcggccacc
     7261 ctggccgacc gctacatcaa cgaccggttc ctgcccgaca aggcgatcga cctgatcgac
     7321 gaggcgggcg cccggatgag gatccgccgg atgaccgcgc cgccagacct gcgtgatttc
     7381 gacgagaaga tcgccgaggc gcgccgcgag aaggaatcgg cgatcgacgc ccaggacttc
     7441 gagaaggcgg ccagcctccg cgatcgtgag aagcagctgg tgggccaacg ggctgaacgc
     7501 gaaaagcagt ggcgttctgg cgatctcgac gtggtcgccg aggtggacga cgagcagatc
     7561 gccgaggtgc tgggcaactg gaccggtatc ccggtattca agctcaccga ggccgaaacg
     7621 acacgcctgc tgcgcatgga agacgagttg cacaagcgga tcatcgggca agaggatgcc
     7681 gtgcgggctg tctctaaggc catccgccgc acccgcgccg ggttgaaaga ccccaagcgc
     7741 ccatcggggt cgttcatctt cgccggcccg tccggtgtcg gtaagaccga gctgtccaag
     7801 gcgttggcca acttcttgtt cggcgacgac gacgcgctca tccagatcga catgggcgag
     7861 ttccacgacc ggttcaccgc gtcgcggctg ttcggtgccc cgccgggata cgtcggctac
     7921 gaggagggcg gccagctcac cgagaaggta cggcgcaagc cgttctcggt ggtgctgttc
     7981 gacgagatcg agaaggcgca tcaggagatc tacaacagtc tgttgcaggt cctcgaggat
     8041 ggccggctca ccgacggcca gggccgcacg gtggacttca agaacaccgt gctgatcttc
     8101 acctccaacc tcggcacgtc cgacatctcc aagccggtcg gcctcgggtt cacccagggc
     8161 ggcggtgaca acaactacga gcggatgaag cagaaggtca acgacgagct caagaagcac
     8221 ttccggccgg agttcctcaa ccgcatcgac gaagtcatcg tcttccacca gctgactcgg
     8281 gacgagatca tccggatggt cgacctgatg atcggccggg tcgccggcca gctcaagagc
     8341 aaggacatgg agctggtgct gaccgacaaa gccaagtcgt tgctggccaa acgcggcttc
     8401 gacccggtgc tgggtgcgcg tccgctgcgg cgcaccatcc agcgcgagat tgaggaccag
     8461 ctctcggaga agatcctgtt cgaggaggtc gggccgggac aggtcgtcac ggtcgacgtg
     8521 gacaactggg atggcgaggg cgccggcgag gacgtggtgt tcacgttcac cggcacccgc
     8581 aagccgccga ccgagccgga cctcgccaag gccggagccc acaccgcggg cggtccgggg
     8641 cccagcgcac agtagtagcg gtttgccaaa acgggcggtt agtctctgac gggactaccg
     8701 cccgtttttg ctgcgctcca agagaagggt ctgcctgtgc tgatcgtcac caccaacgac
     8761 cttccgggct gggagatcca gcgcgtgtgt ggcgaggtgt tcgggctgac cgttcgctcc
     8821 cgaaatgcgt tcgcacaatt cggtgcgggg ctgaagtcca tgttcggcgg tgagctcgcc
     8881 ggaatgacga agaatctggc cgagagccgc aacgaagcga tggggcggct gatcgccgag
     8941 gcgcgcaacc gcggcgggaa cgcgatcgtc gcaatgcggt tcgacacgac cgaaatcggc
     9001 gacgtgtgga ccgagatatg cgcttacggc acggcggtgc aggcggtccc ggtcaccgat
     9061 gccgccaggt acaccgcgaa ccagctcggc tacggcggtc cggctccaca acattgaccc
     9121 gcggtttaca ctgacttctg tcctaacgag catcggaatc tggtgaaagt ccagaacggt
     9181 cgcgccactg tccaaagtca gacccgaagc tcgttggcca gccatcccca ggtgggacgc
     9241 gatattccca gaaggagccg atgtgtccaa tcctcaggcg accggcgccc gtgtccggtc
     9301 ggtcgatcta tcggcaacaa gtgcgacgct ctggttgacg gcgaccgcgt ttctggcgct
     9361 gttggcgatc tacttcgtcg gcgtcgatca gggcgcggtg tcactgttcg gtagtgacat
     9421 gcatgtgcac gagttcgtgc acgacgcgcg ccatctactc ggcttcccct gccattaaaa
     9481 ccggggaaga ccgggctacg tggagaagcg tctgatcgca cgcggtctcc tggcgggctt
     9541 cgccggcgcg ttgctggcgt tcctgttcgc gcgcctgtgc gccgagcccg tcatcggccg
     9601 cgccgtcgct ttcgaggacg gtagaaccga tgccgagaat gcgcacggtg tgcacgagca
     9661 cggcgtggat ttgttcagcc gcggcgtgca atccaacgtc gggctgggtt tcggtgtgtt
     9721 gattttcggt gtcgcgatgg gcgcgctctt cgcggtgctg ttctgcgtcg tctattcgcg
     9781 tgtgagaagc cttgaaccac gcgtactttc gctgctgctc gcggcgggcg cattcgtcgc
     9841 ggtgtacctg gtgccgtttg tgaagtatcc gccgaacccg cccgcggtgg gtcagccgga
     9901 cacgatccgg gcccgcaccg gctggtatct ggtgatggtg ctggcgtcgg tggtgctggc
     9961 gatcgccgcg gtgtggctgg cccggcggct ggccgcccga ttcggtgcgt gggacggtcg
    10021 actgctcgca tcggcgtcgt atgtggtggc ggtcgcggta gtgatggtgc tgttgccgac
    10081 ggtggacgag acgccccaac cactgcgcga cgcgtcgggg gcgatcatct atcccggctt
    10141 ccccgccgac gtcctctatg aattcaggct gctgtcactg ggcacccagc tggtgctctg
    10201 gacgacgatc ggcctggtgt ttgccacact tgccggtcgg gtgctgggtg agcgggccgc
    10261 gagcggacgg gcaccgagca tcgccgcgtg accgaggttg tccggctgac gttggtgtcg
    10321 cacgcgatga ccgatgccat ggcggccgga cggtttcccg gcgacgagcc gctcaacggc
    10381 gtcggccgtc ggcaggtcga agccgttgcg gcccagatca atcgcgacgt ccgccaactc
    10441 accggacccg agcg
//
LOCUS       DASSGQ010000371         2309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1152492,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000371 DASSGQ010000000
VERSION     DASSGQ010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152492"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1754)
                     /gene="pknB"
                     /locus_tag="VFI55_16400"
     CDS             complement(<1..1754)
                     /gene="pknB"
                     /locus_tag="VFI55_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HET7075646.1"
                     /translation="MTTPQHLSDRYELGDILGFGGMSEVHLARDVRLHRDVAVKVLRA
                     DLARDPSFYLRFRREAQNAAALNHPSIVAVYDTGEADTPTGPLPYIVMEYVDGVTLRD
                     IVHNDGPLPARRAIEIIADACQALNFSHQNGIIHRDVKPANIMISTTNAVKVMDFGIA
                     RAIADSGNSVTQTAAVIGTAQYLSPEQARGEPVDARSDVYSLGCVLYEILTGEPPFTG
                     DSPVAVAYQHVREDPVPPSKRHEGISPDLDAVVLKALAKNPENRYQTAAEMRADLVRV
                     HNGEPPEAPKVFTDAERSSLLSSASGGHGGPRTDPLPRQRFDDTDRDRTGGSIGRWVV
                     AVAVLAVLTIVVVIAFNTFGGTTRDVQVPDMRGEVSADAIAALQNRGFKTRTLQKPDS
                     TVPPDHVINTDPGANASVSAGDEITINVSTGPEQREVPDVSSLTYADAVTKLKSAGFS
                     KFKQANSPSTPELSGKVIGTNPPANQTSAITNVVTIVVGSGPETKLVPDVAGQNVDLA
                     QKNLTVYGFSKFSQAQVDSPRPVGEVIGTNPPKGQTVPVDSVIELQVSKGNQFLMPDL
                     TGMFWTDAEPRLRALGWTG"
     gene            complement(1970..2309)
                     /locus_tag="VFI55_16405"
                     /pseudo
     CDS             complement(1970..2309)
                     /locus_tag="VFI55_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein kinase"
BASE COUNT          327 a          721 c          836 g          425 t
ORIGIN      
        1 cctgtccagc ccagcgcgcg taaccgcggt tcggcgtcgg tccagaacat gccggtcagg
       61 tcgggcatca ggaactgatt gcctttggac acctgcagct cgataaccga gtccaccggt
      121 accgtctggc ccttgggcgg attcgtcccg atcacctcgc cgaccggccg ggggctgtcc
      181 acctgcgcct ggctgaactt gctgaagccg tagacggtca ggttcttttg cgcgaggtcc
      241 acattctggc cggcgacgtc gggaaccagc ttggtctcgg gccccgagcc aacgacgatg
      301 gtgaccacgt tggtgatcgc cgacgtctgg ttggccggcg ggttggtccc gatgaccttg
      361 cccgacagtt ccggggttga cggcgagttc gcctgcttga acttgctgaa tcccgcggat
      421 ttgagcttgg tgaccgcgtc ggcgtaggtc aacgacgaga cgtccggcac ctcgcgctgc
      481 tcgggtccgg tggagacgtt gatggtgatc tcgtcgccgg cgctgaccga agcattggcg
      541 ccggggtcgg tgttgatgac gtgatcgggc ggtaccgtcg agtcaggctt ctgcagggtg
      601 cgcgtcttga agccccggtt ctgcaatgcg gcgatcgcgt cggcggacac ttcgccccgc
      661 atgtcgggaa cctggacgtc gcgggtggtg ccaccgaacg tgttgaaggc gatgacgacg
      721 acaatcgtca acaccgccag caccgcgacc gcgacaaccc aacgaccgat cgaaccgccg
      781 gtccgatcac ggtcggtgtc gtcgaaacgc tggcgaggca gcggatcggt gcgcggacct
      841 ccgtggccgc ctgacgccga ggacagcagc gagctccgct cggcgtcggt gaacaccttc
      901 ggcgcctctg gtggctcacc gttgtgcacc cggaccaggt cggcccgcat ttccgccgcg
      961 gtttggtagc ggttttccgg attcttggcc agcgccttga ggacgacggc gtcaaggtcg
     1021 ggcgagatgc cctcatgccg tttcgacggt ggaaccgggt cttcgcggac gtgctggtag
     1081 gcgaccgcga cgggcgaatc accggtgaaa ggcggttcac cggtgaggat ttcgtaaagg
     1141 acgcagccga gcgagtagac gtcggagcgg gcgtcgaccg gttcgccgcg ggcttgttcg
     1201 ggcgatagat actgcgcggt cccgatcacg gccgcggtct gggtgacgct gttgccgctg
     1261 tcggcaatcg cccgcgcaat gccgaagtcc atcaccttca ccgcgttggt ggtgctgatc
     1321 atgatgttcg ccggcttgac gtcgcggtgg atgatgccgt tctggtggct gaagttcagc
     1381 gcctggcagg cgtcggcgat gatctcgatg gcccgccggg ccgggagcgg gccatcgttg
     1441 tgcacgatgt cgcgcaaggt gacgccgtcg acgtactcca tcacgatgta gggcagcggg
     1501 ccggtgggcg tgtccgcctc gccggtgtcg tacaccgcga cgatggacgg gtggttcagc
     1561 gccgcggcgt tctgcgcttc acggcggaag cgcaggtaga agctggggtc gcgggccagg
     1621 tcagcgcgca gcaccttgac ggcgacgtcc cggtgtaaac gaacgtctcg ggccaggtga
     1681 acctcggaca ttcccccgaa gccgaggatg tcgcccagtt catagcgatc ggacaggtgc
     1741 tgcggggtgg tcattgcggt gtctcgtgtt ggggcggcga tgggctgagc gccggtgaca
     1801 caatccagct cgatccggtg atccggcgct ccgccccgcg cttgccgtgc ccccacccct
     1861 gggtgcttcg gcctgcgtcg gccgggctgg atcccattgc ccggctcctc ctcaggccgc
     1921 tgtggcctgc atcgttgacc gggctggata gccgcggcct gctgtggaat ccagaattac
     1981 ctatgctcgc acgatccgtc caattgagcc gcagacccgg tccggcgccg gaaggcgtct
     2041 tgctgggcgg tggcggcgcg gtcgcgggtg gggtccccgt gtcggtgacc gtcgggggcg
     2101 gtgtctgctg ttcgttttct gtgcgggaat tgatgacgat gagcaccgcg atgatgatcg
     2161 ccagtgctcc aagcacgccc gcggcccaca gcagcgcgcg ctgacccgac gagaaggtgc
     2221 gccgagctgg cgccggccgg tggccgccgg tggtgggccg ggaccggcgg gtcgtggggg
     2281 tgcgggcggg cacggccgcc accctggtc
//
LOCUS       DASSGQ010000372        10622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_149602,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000372 DASSGQ010000000
VERSION     DASSGQ010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10622
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149602"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFI55_16410"
     CDS             <1..190
                     /locus_tag="VFI55_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7075647.1"
                     /translation="TGEVRKDLDVKAAGEWFARILFSLFSTPSPIRDMDDPEVAAEFV
                     RAHLVRGFAGDRPRPRRT"
     gene            complement(273..770)
                     /locus_tag="VFI55_16415"
     CDS             complement(273..770)
                     /locus_tag="VFI55_16415"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7075648.1"
                     /translation="MPSSHWHKLYTADINASPAVLFGLVADMPNYGRWLPGSASYGST
                     TDVEPYPVRLGSRYRDGKPGEPGKDWRGEVIGFQPPGSIDFQHTIYVRQLRAIIDTHI
                     HYSFEPAGGATRVRRWLLLAIDMPLLLRPLRRPIISAFDKENLRTIAALAKYAEAHRD
                     EWPGD"
     gene            complement(1255..2625)
                     /locus_tag="VFI55_16420"
     CDS             complement(1255..2625)
                     /locus_tag="VFI55_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPE domain-containing protein"
                     /protein_id="HET7075649.1"
                     /translation="MDFAVLPPEINSGHMYAGPGSGSMVAAAAAWDGLAAQLHSTAAS
                     YSSVISGLTAGWQGASSAAMAAAAAPYAAWISGTAAAAEQAAAQARAAASAYQTAFAA
                     TVPPPVIAANRSQLAALMATNLFGQNTPAIAATEAQYADMWAQDAAAMYGYAGSAALA
                     TQLTPFTEPPQTTNTAGAAGQAAAIAHAVGTSAGTNAQSALTQLISAVPQILQSVANP
                     PSSITTIAGAAESIGGLITGPAQGAPVVTVTSALGAYSSYLGGILDAAGPAGASSPGL
                     GALAAGLESGMPAPGSASFGAAGSATSAGMGSAGAVGALSVPPSWAAATTMVRLAATV
                     LHGTSAAAAPAVTVESAGSASGQLALASVAGAALGASVPRAISPTAIRGGRPTSDKDS
                     QTPDKLKRVLAELSQKPESVQHWHTDKAHLESLLDQLSKKPGIHAVHLSAGDKPRATP
                     PKAQWG"
     gene            complement(2637..2936)
                     /locus_tag="VFI55_16425"
     CDS             complement(2637..2936)
                     /locus_tag="VFI55_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HET7075650.1"
                     /translation="MSFVTTQPALLTEAAGNLTGIGSAMNTENSAAAARTTAVVPAAA
                     DEVSALSAAQFAVHAQMYQAISAQAAAIHEMFVNTLLTSAGSYAATEAANAVAAT"
     gene            complement(3210..4430)
                     /locus_tag="VFI55_16430"
     CDS             complement(3210..4430)
                     /locus_tag="VFI55_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPE family protein"
                     /protein_id="HET7075651.1"
                     /translation="MDFGALPPEINSARMYAGPGAGPMLGAAAAWDGLAAELHSTAAS
                     YGSVITGLAVGWQGPSSASMAAAVAPYTAWMSATAAQAEQTATQARAAVAAYETAFAA
                     TVPPPIVAANRALLVSLIATNFLGQNTPAIAATETHYAEMWAQDATAMFGYAGSSATA
                     SQVTPFTEPPQTANPAAAGGQAAAVAHAVGTSAGTDTQSVLTQLMSAVPQSLQSLASP
                     AVSAAADPPSPLSILGSLTGPFSPVSLASLGLGGYLDSAEPYGLASVGSAVAGLSQKV
                     SALPAAGSGGLLEGGLGSGTRVVGSTGGVSADIGRASLVGRLSVPQSWASAAPAIRPV
                     AAVLPDSGLGAAPVMVAADDQGGLFSNMALSSVAGRAMAGTGGTAARFIGVGGGAYSG
                     QATTATIIVIPADE"
     gene            4648..5148
                     /locus_tag="VFI55_16435"
     CDS             4648..5148
                     /locus_tag="VFI55_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7075652.1"
                     /translation="MAKHSTSPGHSGLGSVPIPAAALGGPISHAIFRIARLHKMMAGN
                     LLRRLGLHPGQEMVMMYLWELGPQRQTDLIRLMDSDAATMTRTIQRLEHAGFVRRSPC
                     ADDKRAYLIESTAAGRALRPQVEQIWIQLEEIVVGGAGERERQTTQRVLKSLEERLTH
                     AAESRV"
     gene            complement(5791..6759)
                     /locus_tag="VFI55_16440"
     CDS             complement(5791..6759)
                     /locus_tag="VFI55_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HET7075653.1"
                     /translation="MAGVFANTLRPAILAAGRRQTLRRAAERLPVTRSVVHRFVPGET
                     IASALDSVAALCDSGRYASIDYLGEDVTDADDADAAVQIYLDLIAELGRRNDPADGRV
                     RPLEVSVKLSALGQSLERDGEKIARENAWSICAAAQRAGVWVTVDAENHTVTDSTLSI
                     VRDLRADFPWLGTVLQAYLRRSLGDCEEFAVAGARIRLCKGAYDEPASVAYRDDAGVT
                     DSYLRCLRVLMAGRGYPMVASHDPVVISAVPEIAAQSGRGAGDFEYQMLYGIRDGEQR
                     RLAAAGNQVRVYVPFGTQWYGYFMRRLAERPANLTFFLRALAQRGH"
     gene            complement(6759..8390)
                     /gene="pruA"
                     /locus_tag="VFI55_16445"
     CDS             complement(6759..8390)
                     /gene="pruA"
                     /locus_tag="VFI55_16445"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HET7075654.1"
                     /translation="MDAITQVPMPANEPVHDYAPRSPERSRLIAELTALAADPIDLPH
                     VIGGKHRMGDGERVDVVPPHRHAARLGTLTNAVHADATAAIEAAMSAKKSWAAMPLDE
                     RAAVFLRAADLLAGPWREKIAAATMLGQSKSVYQAEIDSPCEQIDFWRFNVAFARQIL
                     AQQPISGPGEWNRSDYRPLDGFVYAITPFNFTSIAGNLPTAPALMGNTVVWKPSITQT
                     LSAYLTMQLLEAAGLPPGVINLLTGDGFAVSDVALADPRLAGIHFTGSTATFQHLWQQ
                     VGANIGRYHSYPRLVGETGGKDFVVAHASARPDVLCTALIRGAFDYQGQKCSAASRAF
                     IAHSVWQQMGDAFLGATAALRYGDVTDLTNYGGALIDRRAFVKNVNAIERAKGTPGVT
                     VAVGGEYDDGVGYFVRPTVLLSDDPTDEAFATEYFGPLLSVHVYPDDQYERILDVIDT
                     GSRYALTGAVIADDRAAVLTAQDRLRFAAGNFYVNDKPTGAVVGRQPFGGSRGSGTND
                     KAGSVLNLLRWTSARTLKETFVPATDHNYPHMGSD"
     gene            8475..10082
                     /locus_tag="VFI55_16450"
     CDS             8475..10082
                     /locus_tag="VFI55_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7075655.1"
                     /translation="MRAAGVGLGQLLLALDATLVSLVAAPRGLDLPVGSAALIDSDDV
                     RLGLAAAAGAADVFFLLGVGDDEALRWVDKQARERVPVATFVKQPSDALVARAVAAGS
                     AVVAVEPRARWERLYQLVNHVLEHHGDRGDPVDDSGTDLFGLAQSLADRIHGMVSIEN
                     AQSHVLAYSASNDEADELRRLSILGRAGPPEHLEWIDQWGIFDALRSSGEVVRVAERP
                     ELGLRPRLAIGIYQPSADARRPPVFAGTIWVQQGSQPLADDAEEMLRGAAVLAARIMS
                     RLAARPSTHAQRVQQLLGFADGEPDDLAAIARELGLAADGAAALIGWDTPGSPGAGAR
                     QVRLGDVLALSASAFRRDAQVATRGSRIYVLLPQTQTRSVTSWVRGTISALRTELCVE
                     LRAAIAAPVASLADVAAARAEVDRVLDSAERHPVSIGQVTSLAEARTTVLLDEVVTLV
                     GADERLVDPRMRDLRARDPVLAETLRAYLDSFGDIGAAALWLQVHPNTVRYRVRRIEK
                     LLSTSLSDPEVRLLFSLGLRVLERTRS"
     gene            complement(10429..>10622)
                     /locus_tag="VFI55_16455"
     CDS             complement(10429..>10622)
                     /locus_tag="VFI55_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075656.1"
                     /translation="LEKYVAAASTTRVQVKQFLWGVREEFRCPSGGARRLNLGCPRAA
                     SQQGILTVLNRHTIGGRWV"
BASE COUNT         1771 a         3503 c         3535 g         1813 t
ORIGIN      
        1 caccggcgaa gttcgcaagg acctggatgt gaaagccgcc ggtgaatggt tcgcgcgcat
       61 actgttctcg ctgttcagca cgccgtcgcc gattcgggac atggacgatc cggaagtggc
      121 tgccgagttc gtgcgcgcgc atctggtgcg cggttttgcc ggcgatcgtc cacggccgag
      181 gcggacgtag gtctcgaccg acgttggctg ccggcggctt aactccggcg gagccccggg
      241 gggccccgga gccccggcga ccttgcgttc ggtcagtcgc ccggccattc gtctcgatgc
      301 gcctcggcat acttggcgag cgcggcaatg gtccgcaggt tctctttgtc gaacgcgctg
      361 atgatcggcc ggcggagggg acgaagtaga agcggcatgt cgatggccag cagcagccag
      421 cgccttaccc gggtcgcgcc acccgcgggt tcgaacgaat agtggatgtg ggtgtcaatg
      481 attgcccgca attgccgcac atagatcgtg tgctggaagt caatcgatcc tggtggttga
      541 aatccgatga cttcgccccg ccagtctttg ccgggttcgc cgggtttgcc gtcgcgatag
      601 cggctgccca gccgcaccgg atacggctcg acgtcggtgg tgctgccgta cgacgccgag
      661 cccggcaacc agcgaccgta gttgggcatg tcggccacaa gcccgaacag caccgcaggc
      721 gacgcgttga tatcggccgt gtacagcttg tgccaatgcg agctgggcat gtcagctcct
      781 ttcggctacc gaggctacta gtgtgcatca gcggcttaaa acgtgcggcc tgccggtgaa
      841 tagtttgcgc gcatatgttc tcgctgttca gcgcaccctt gtcgacgctg aacatagaca
      901 acccccaagt ggcagccgaa ttcgtgcgcg cacgcgtgat acgcgggttc gccggcgatc
      961 gcgcgcggcc gaggcgcggt taggcttcaa ctgcgctgtt gggaccaaac ggtctcggca
     1021 acatcaaccg gtcggccctg gccgggcagt tcgtgcggtg gcgaacgctc gagcagactt
     1081 tgggcggcgt ggcagttttc ctaggggtag agcctaatcg cagcacaatg gcctagacgc
     1141 tgtgcgagtc tctcatgcga acagtcagca gcgtcgacac tgaccgcgag atacgcgcca
     1201 aaaacccaac acgctgcgga tgacacagaa tgtgggacat tgatccgatt gcggttatcc
     1261 ccactgtgcc ttgggcggtg tggcgcgggg cttgtcgccg gccgacaaat gcaccgcgtg
     1321 gatgcccggc ttcttcgaca gctggtccag cagactttcc aggtgagcct tgtcggtgtg
     1381 ccagtgctgc accgattccg gtttctgcga cagttcggcg aggacacgtt tgagtttgtc
     1441 cggcgtctgg ctgtccttgt ccgaggtggg tcggccgcct cggattgctg tcgggctgat
     1501 ggcacgagga acgctggcgc ctaaggcggc accggccaca ctcgccaggg ccagctgacc
     1561 agacgcggac cctgcgctct ctacggtgac cgccggagcc gcagccgcac tggttccgtg
     1621 taacacggtg gcggccagtc tgaccatcgt ggtggccgcc gcccagctcg gcggcaccga
     1681 cagcgccccc accgcgcccg cgctgcccat gcccgccgag gttgcggagc cggccgcacc
     1741 gaagcttgcc gagcccggcg cgggcattcc tgattccagc cccgccgcca aagctcccaa
     1801 ccccggactc gatgctccag ctggacccgc tgcgtccaat atgcctccca aatagctgga
     1861 ataagcgccc aaggcagagg tgacggtgac taccggggca ccttgcgcgg gaccagttat
     1921 aagtccgccg atagactcgg cagcgccggc gatcgtcgta atcgatgacg gcgggttggc
     1981 gacgctttgc agaatctgag gcactgccga gatcagttgt gtcaatgccg attgggcgtt
     2041 ggtgccggcc gatgtgccaa cggcgtgggc gatcgcagcg gcctggccgg ccgcccccgc
     2101 cgtgttggtg gtctgcggcg gctcggtgaa cggtgtcaac tgggtggcga gcgccgcgga
     2161 gccggcgtag ccgtacattg cggcggcgtc ttgggcccac atgtcggcgt actgcgcctc
     2221 ggtggccgcg atcgccgggg tgttttggcc gaacaggttg gtcgccatga gcgccgccag
     2281 ctggctgcgg ttggcggcaa tcaccggcgg gggtaccgtc gcggcaaacg cggtctggta
     2341 ggcgctcgcc gccgccctgg cttgcgcagc ggcctgttcg gcggccgcgg cggtgccact
     2401 gatccacgcc gcatagggtg cggccgcggc cgccatcgcc gccgacgacg cgccctgcca
     2461 ccccgccgtc aatcccgaaa tcaccgaaga gtaggacgcc gcggtggagt gcaattgggc
     2521 ggctagcccg tcccaggccg ccgcggccgc caccatcgac cccgagcccg gaccggcata
     2581 catgtgaccg gagttgatct ccggcggtag caccgcgaag tccatcgcca acgcccctag
     2641 gtcgccgcga ccgcgttggc ggcctcggtg gcggcgtagg acccggcgct ggtgagcagc
     2701 gtgttgacaa acatttcgtg aatcgcggcg gcctgtgcgc tgatcgcctg atacatctgg
     2761 gcgtgtacgg caaactgcgc cgcgctcagc gccgaaacct catcggccgc agcgggaacc
     2821 accgccgtcg ttcgagccgc tgcggccgag ttctccgtat tcatcgctga gccgataccc
     2881 gtcagattgc cggcggcctc agtcagcagt gcgggttgtg tagttacgaa agacatgtgt
     2941 cctcctttgt cttgtctgtt tgagtcttcg tggatcaccc ggaggtcgcg cggcgcaact
     3001 gggtgtcaaa cttttgtcga ccggcaatcg gcccaaaatc cgctcgcagc aaccatgttg
     3061 gggagaatcg cccggcactt tgcctgccac gcggtggcgg cggccggacg gtggagccgt
     3121 acgcgtcggt atcgccgaac tggaagtgga tcatcgtcga cgtgtctcca cgggtgtggt
     3181 taggcggcta gacaggtgcc gtgcggcggt cattcgtctg ccgggatcac gatgatggtg
     3241 gcggtggtgg cctgaccgga gtacgcaccg ccgcccacgc cgatgaaacg ggcagcggtg
     3301 ccgccggtgc cggccatggc gcgtccagcc acactcgaca gggccatatt gctgaacaag
     3361 ccgccctggt catccgcagc gaccatgacc ggggcggcac ccagaccgga gtcgggcagc
     3421 accgcggcga ccggcctgat cgccggggcg gccgaggccc aactttgcgg caccgacagg
     3481 cgcccgacca agctggcgcg accgatatcc gccgagaccc cgcctgttga gccgaccacc
     3541 cgcgtcccgg aacccagccc gccctccagc aaccccccgc tgcccgccgc tggcaatgcc
     3601 gacaccttct gggataagcc ggcgacggcg ctcccgaccg aggccaggcc gtatggctcc
     3661 gcggaatcca gataaccgcc gaggcccagg ctggccagcg agaccggcga gaatggcccg
     3721 gtgagactgc caagtatcga cagcggcgat ggcggatcag ccgccgctga tacggcgggc
     3781 gatgccaggc tttgcagcga ctgcggcacc gccgacatca gttgggtcaa taccgattgg
     3841 gtatcggtgc cggccgacgt gccgacggcg tgggcgaccg cggcggcctg gccacccgca
     3901 gcagccgggt tggcggtctg cggcggctcg gtgaacggcg tcacctggga ggcggtcgcc
     3961 gacgagccgg cgtagccgaa catcgccgtg gcatcctggg cccacatctc ggcgtagtgc
     4021 gtctcggtgg ccgcgatcgc gggggtgttc tgcccgagga agttggtcgc gatcagcgac
     4081 accaacagag cacggtttgc cgcgacaatc ggtggaggca ccgttgccgc gaacgcggtc
     4141 tcataggcgg cgacggccgc cctggcctgg gtggcggtct gctcggcctg cgcggcggtg
     4201 gcgctcatcc atgccgtata cggtgcgacc gctgccgcca tcgacgccga cgacggaccc
     4261 tgccacccga cagctagccc tgtgatcacc gacccgtaag acgccgcggt ggagtgcaat
     4321 tcggcggcca acccgtccca ggccgctgcg gcacccagca tcggtcctgc gcctggacct
     4381 gcatacattc gcgcggagtt gatctctggc ggtaaagctc cgaaatccat cactgccctc
     4441 ctgcccgctt gcgcgaaagc tctcccacgg gggcgccgac gtccgccgtg aaaccaagtg
     4501 gctcgggtcg accgctcatg gttgcctccc tctcatcaga ttacctgttc gaacagttat
     4561 attgcaatac agatattcca aactttttga tatgtctcta atagttgtta agctgaccgg
     4621 gcaggatcat ggagggtgag acggcaaatg gcaaagcaca gcacgtcacc ggggcacagc
     4681 ggactgggga gtgttcccat cccggcggcc gcacttggtg ggccgatcag tcatgcgatc
     4741 ttccggatcg cgcgccttca caagatgatg gccggcaatc tgctacgtcg cctcggcctg
     4801 caccctggcc aggagatggt gatgatgtac ctgtgggaac tgggtcccca gcgccagacc
     4861 gacctgatcc gcttaatgga ctcagacgcg gccaccatga ccaggactat ccagcgcctc
     4921 gagcacgccg gtttcgtccg gcgctcgccc tgcgccgacg acaagcgggc ttacctcatc
     4981 gaatcaaccg cagcgggtcg cgcgctgcgc ccgcaggttg agcagatatg gatccagctc
     5041 gaggagatcg tcgttggtgg cgctggtgaa cgcgagcggc agacaactca gcgcgtccta
     5101 aaaagcttgg aggaacggct tacccacgcc gcagagtctc gggtctagct ggtttgtacc
     5161 gatcgatcat ggctttgtcg cgcggtatgg ccgcttcacg gccacgcttc gatcaacggg
     5221 catgacacgc cgaggcccgt tcgctatgcg ggcgcgtcac gcgcgggcgc acagttacgc
     5281 agcttggtcg gagattgcca cgaggacgac ggctgtctta tcctcccggg ccgttgcggc
     5341 cgggcccgca ccacacccgt tgcaggcaag cacatttcgc gcgaggggac cgtgccagac
     5401 accggtagcc ggggacgaac cccagcattg gttaccggtg tctggagcgg cggctaggtc
     5461 accaagtgaa ccgtggcggc cctccgtcac gctgatcagt gggtcagggc ggcgtcgagg
     5521 ttgttcaaca ccccgagctg agcaaagaag atcgccacgg agatggttcc actcaaatca
     5581 ccggaatcga tgtacgcccg tcaaacctgg cacccggcgt tccggtcggc gacagcctgc
     5641 cgacgtgcac gaccaccggc agcgccaacg caccagaggt catatcacca gtacctgtga
     5701 acggcttccc gggcatcttt aacaccgtta cgttcccttc ccgctcggtc cgatttacat
     5761 caatttctga ccgacgattg caacggcgac tcagtgaccg cgctgcgcca atgcccgcaa
     5821 aaagaacgtc aggttggccg gtcgctcggc cagccgccgc atgaagtagc cgtaccactg
     5881 ggtgccgaat ggcacgtaca cccgcacctg gttgccggcc gcggccagcc gtcgctgctc
     5941 gccgtcgcgg atgccgtaca gcatctggta ttcgaaatcc ccagcaccac ggcctgattg
     6001 ggcggcgatc tcgggcaccg cggagatgac gacgggatcg tgcgaggcga ccatcggata
     6061 cccgcggccg gccatcagga cccgaagaca ccgcagatag gagtcggtga ccccggcgtc
     6121 gtcccggtag gccaccgatg cgggttcgtc ataggcgccc ttgcagagcc gtatccgggc
     6181 ccccgcgacg gcgaattcct cgcaatcccc caggctgcgt cgcagatagg cctgtagaac
     6241 tgtgcccagc cacgggaaat ccgcccgcaa atcgcggacg atcgacagcg tcgaatctgt
     6301 cacggtgtgg ttctcggcat ctaccgtcac ccacacgccg gcgcgctgcg ccgcagcgca
     6361 aatggaccag gcgttctccc gcgcgatctt ctcgccgtcg cgctcgagcg actgtcccag
     6421 cgccgacagc ttgaccgaca cctccaatgg ccggacgcgg ccatcggcgg gatcgttgcg
     6481 gcggcccaac tcggcgatca ggtccaggta gatctgcact gcggcatcgg cgtcatcggc
     6541 gtcggtgaca tcttcgccga ggtagtcgat gctggcataa cgtcccgaat cacacagcgc
     6601 agcaacacta tccagcgcgc tggcgatcgt ctcaccgggc acgaaccggt gcaccacgct
     6661 acgggtgacc ggcaaccgtt cggcagcgcg gcgcaacgtc tggcgccggc ctgcggccag
     6721 aatcgccgga cgaagggtgt tggcgaacac cccggccatc agtcggaccc catgtgcggg
     6781 tagttgtggt cggtcgccgg aacgaatgtt tccttgaggg tgcgtgccga tgtccagcgc
     6841 agcaggttca gcaccgatcc ggccttgtcg ttggtacccg agccgcgcga acccccgaac
     6901 ggctgacgcc cgaccaccgc gccggtcggc ttgtcgttga cgtagaaatt ccccgcggcg
     6961 aagcgcagcc gatcctgtgc ggtcagcacg gcagcacggt cgtcggcgat gaccgcgccg
     7021 gtcaacgcgt agcgggatcc ggtatcgatg acgtcgagaa ttcgttcgta ctgatcgtcg
     7081 gggtagacat gcaccgacag cagcggaccg aaatactcgg tcgcgaacgc ctcgtcggtc
     7141 gggtcgtcgg acagcaacac ggtcgggcgc acgaaatacc cgacgccgtc gtcgtattcg
     7201 cccccaaccg cgacggtcac gccgggcgtg cctttcgccc gctcgatggc gttgacattc
     7261 ttgacgaagg cacgccggtc gatcagcgcc ccgccgtagt tggtcagatc ggtgacatcg
     7321 ccgtagcgca gcgcggcggt cgcacccagg aaggcgtcgc ccatctgctg ccacaccgaa
     7381 tgcgcgatga atgcccgtga cgccgccgag catttctgac cctggtagtc gaacgcgcca
     7441 cgaatcaacg ccgtgcacaa cacgtccggc cgtgccgatg cgtgtgcgac gacaaagtct
     7501 ttgccgccgg tctcgccgac caggcgcgga tagctgtggt agcggccgat attggcgccc
     7561 acctgctgcc acagatgctg aaacgtggcc gtcgacccgg tgaagtggat accggccaat
     7621 cgcggatcgg ccagtgccac gtcggaaacc gcgaagccgt cgccggtcag caggttgatc
     7681 acgccgggcg gcaagcccgc ggcctccagc agttgcatgg tcaggtacgc cgacagcgtc
     7741 tgggtgatcg atggcttcca caccactgtg tttcccatca gcgccggcgc cgtcggcaga
     7801 ttgccggcga tcgaggtgaa gttgaacggc gtgatcgcat agacgaagcc gtccagcggg
     7861 cggtagtcac tgcgattcca ctcgccgggt ccgctgatcg gctgctgcgc caggatctga
     7921 cgggcgaatg ccacgttgaa ccgccagaaa tcaatctgct cgcaaggcga atcgatctcg
     7981 gcctggtaga cggacttgga ttggccgagc atggtcgcgg cggcgatctt ctcccgccac
     8041 ggcccggcca gcaggtcggc ggcccgcagg aacaccgcgg cgcgttcatc gagaggcatt
     8101 gccgcccaag actttttcgc ggacatcgcg gcctcgatcg ccgccgtcgc atccgcgtgg
     8161 acggcgttgg tcagggtccc cagcctggcg gcatgtcggt ggggcgggac gacatcgacg
     8221 cgctcaccgt cgcccatgcg gtgcttgccg ccgatgacgt gcgggaggtc aatcggatcg
     8281 gcggccagcg cggtcaactc ggcaatcagc cggctgcgtt ccggggatcg gggtgcatag
     8341 tcgtgcaccg gctcgttggc cggcatcggt acctgggtga tggcatccat gctcccaagg
     8401 atccccggca cggcacacca aattattggc ggatcagaca agatatacat gatcgggtag
     8461 tacgatcgga caatatgcgg gcggctggtg tcgggctggg tcagctgttg ctggcgctgg
     8521 acgcgacgct ggtcagcctg gtggcagccc cgcggggact ggaccttccg gtcgggtcgg
     8581 cggcgctgat cgactccgat gatgtgcggc tgggcctggc cgccgcggcg ggcgcggccg
     8641 acgtcttctt cctgctcggt gtcggcgacg acgaagcgct gcggtgggtt gacaagcagg
     8701 cacgcgagcg ggtgccggtg gcgaccttcg tcaagcagcc gtcggacgcg ctggtagcca
     8761 gggcggtcgc ggccgggtca gcggttgtcg ccgtggagcc gcgggcccgt tgggagcggc
     8821 tgtaccagct ggtcaaccac gtgttggaac atcacgggga ccgtggcgac ccggtggatg
     8881 actcgggtac cgacctgttc ggtttggccc agtcgctggc cgaccgcatc cacggcatgg
     8941 tcagcatcga aaacgcccaa tcacacgtgc tggcctattc ggcttccaac gacgaagccg
     9001 acgagctgcg ccgcctttcc atcctgggcc gcgccgggcc acccgagcat ctggagtgga
     9061 tcgaccaatg gggcatcttc gatgcgctgc gatccagcgg cgaggtggtg cgtgtcgccg
     9121 agcgtcccga gttggggctg cgtccgcggc tggcgatcgg gatttaccag ccatccgccg
     9181 atgcgcggcg gcctccggtg ttcgccggca ccatctgggt gcagcaaggt tcgcaaccgc
     9241 tggccgacga cgccgaggag atgttgcggg gtgcggcggt gctggccgcc cgcatcatgt
     9301 ctcggctggc ggcccggccg tccacccatg cgcagcgggt gcagcagctt ctcggattcg
     9361 cggatggcga gcccgacgac ctcgccgcga tcgcccgcga acttggtctc gccgccgatg
     9421 gcgccgccgc cctgatcggt tgggacaccc caggcagccc cggagccgga gcccgtcagg
     9481 tgcggctggg tgacgtcttg gcgttgagcg ccagtgcttt tcgccgcgat gcgcaggttg
     9541 cgacgcgtgg ctcgcgaatc tatgtgctgc tgccccagac tcagacccgg tcggtcacct
     9601 cgtgggtccg cggcacgatc agcgcgctgc gcaccgaact ctgcgtcgag ctaagggccg
     9661 ccatcgccgc cccggtcgcg agcttggccg acgtggccgc ggcgcgggcg gaggtggacc
     9721 gcgtgctcga tagcgctgag cgacacccgg tttcgatcgg ccaggtgacc tcgctggctg
     9781 aagcacgcac caccgttttg ctcgacgagg tcgtcactct ggtcggcgcc gacgagcggc
     9841 tggtcgatcc gcggatgcgc gacctgcgcg cgcgggaccc cgtgctggcc gagacgctgc
     9901 gggcctacct ggacagcttc ggtgacatcg gcgccgccgc gctgtggctt caggtgcatc
     9961 ccaacaccgt tcgctatcgc gtgcgccgga tcgagaagtt gttgtcgacc tcgctgtccg
    10021 atcccgaggt gcggctgctg ttttcgctcg ggctgcgggt cttggagaga acccggtctt
    10081 agagagaacc gccggtgtcg gtgccacttg cgattaggcg cgccgctgat gacgccgagg
    10141 atgaccggca agacgcacgt acacccagtt gatggcccag ccgtgaccga tcgcgataag
    10201 cactaggccg acgatgaaaa cgatattgcc cagaagtgga cggtcctcgc ccaagcgctt
    10261 cccccacacc gaggccgcag caccagccgg acccatcacg aaccaccaaa aggggtgccg
    10321 cctgataagg ctcaatgagc ttctgtcgtc tgccatttct gttggcgctc ctcatacacc
    10381 gcccgttgtt cagtctagac tgcacaatcg agacattcac tgggatgact acacccatcg
    10441 accgccaatg gtgtgccggt tcaatacggt caggatcccc tgctgactcg ccgcccgtgg
    10501 acagcccagg ttgagccgac gggcaccacc cgaagggcac cggaattctt cccggacgcc
    10561 ccaaagaaat tgcttgacct ggacgcgtgt tgtggacgcg gcggcgacgt atttttccag
    10621 cc
//
LOCUS       DASSGQ010000373         4227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_999597,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000373 DASSGQ010000000
VERSION     DASSGQ010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999597"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /gene="rpsA"
                     /locus_tag="VFI55_16460"
     CDS             <1..171
                     /gene="rpsA"
                     /locus_tag="VFI55_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216146.1"
                     /note="in Escherichia coli this protein is involved in
                     binding to the leader sequence of mRNAs and is itself
                     bound to the 30S subunit; autoregulates expression via a
                     C-terminal domain; in most gram negative organisms this
                     protein is composed of 6 repeats of the S1 domain while in
                     gram positive there are 4 repeats; the S1 nucleic
                     acid-binding domain is found associated with other
                     proteins; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET7075657.1"
                     /translation="KMHTAQMEKFAAAEAAGHGGGSSSPGNGVSSEKSAGGSLASDAQ
                     LAALREKLAGNA"
     gene            196..1437
                     /gene="coaE"
                     /locus_tag="VFI55_16465"
     CDS             196..1437
                     /gene="coaE"
                     /locus_tag="VFI55_16465"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET7075658.1"
                     /translation="MLRIGLTGGIGAGKSALSATFAECGAIIVDGDVIAREVVQPGTE
                     GLASLVDAFGADILLADGSLDRPALAAKAFRDDEARGVLNGIVHPLVAKRRTEIVAAV
                     AEDAGDVVVVEDIPLLVESGMAPLFPLVVVVHADIDVRVRRLVEQRGMSEDDARARIA
                     AQADDEQRRAVADVWLDNSGGPGELVGRAHDVWNNRILPFAHNLSARQAARAAARLVP
                     PDPSWPDQARRILARLNTTCGHRAVRIDHIGSTAVPAFPDFQAKDVIDIQITVESLAV
                     ANELAGPLLSAGYPRIERITQDVAKAPRVDARSTVERYDHNDDPSLWHKRIHASADPG
                     RPTNVHIRVDGWPNQQFALLFVDWLAANPDVRADYLAVKRAAEQRAHGDTADYVAVKE
                     PWFLDAYPRAWQWADSTGWRP"
     gene            complement(1455..1895)
                     /locus_tag="VFI55_16470"
     CDS             complement(1455..1895)
                     /locus_tag="VFI55_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HET7075659.1"
                     /translation="MRAVEEYTVRPWGLYVARPTPGRAQFHYLESWLLPSLGLRATVF
                     HFNPGHERDHDYYLDVGEYTPGPRVWRADDHYLDIEVRTGSEATLADVDELLDAVRHG
                     LLNPEDAERAVQRAVAAVDGLARNGYDLHGWLAGKGMELTWRGA"
     gene            complement(1993..>4227)
                     /locus_tag="VFI55_16475"
     CDS             complement(1993..>4227)
                     /locus_tag="VFI55_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7075660.1"
                     /translation="PPPRQPRQAPPQRIHTSGMPAPARPPIGTPPDQVTVVNTGSVAR
                     SHPTPAAGSDTGPPAQLESTPAAESTVINAKTADVSNLATRFVKLLTPRSSAAAGTTG
                     AMTIGRAADNDFVVSDVLASRHHATLVPTPLGTEIRDNSVNGTFVNGTRVGSAILTDD
                     DVVTIGNVDLVFRDGTLVPGSAAAARTGGLEVRNVKYVVDNGKQLLDNISLSARPGTL
                     TAVIGGSGAGKSTLARLIAGYARPTSGSVTFEGHDIHADYASLRSRIGMVPQDDVVHR
                     QLTVNQALGYAAELRLPPDTSKADRAKVVTQVLGELDLTKHADTRVDKLSGGQRKRAS
                     VALELLTGPSLLILDEPTSGLDPALDHQVMTMLRQLADAGRVVIVVTHMLSYLDVCDQ
                     LLLVAPGGKTAYCGPPDQIGDAMGTSNWAKIFTKVGADPEEANRRFIAQRDAQKRPQK
                     LLPDSTDEPDDLGKPVHTSVRRQISTVARRQVRLVVADRAYFIFLAVLPFILGVLSLT
                     VPGTSGFGVPGPGASTPDESAQILNLLLLAAAFMGTALTIRDLVGERAIFQREQAVGL
                     STSAYLLAKSMVYCGFAIVQSAITTTIVVIGKGAPTQGAVLLGHGTFGATIELFLTVA
                     GTCVTSAILGLAISSLVRSSEQIMPLFVVSIMAQLVLCGGMVPVTGRLGLDQLSFVMP
                     ARWGYAAAASTVDIRHLVPASLLPQDRFWQHTSKIWLTDMGMLAALSVFYAGFVRWKL
                     RLRR"
BASE COUNT          675 a         1387 c         1493 g          672 t
ORIGIN      
        1 aagatgcaca ccgcgcagat ggagaagttc gcggcggccg aagcggccgg acacggcggt
       61 ggcagctctt cgcctggcaa cggtgtttct tcggagaagt cggcgggtgg atcgctggcc
      121 agcgacgccc aattggcggc cctgcgggaa aagctcgccg gcaacgcata gtcccgcgcc
      181 catcgcattc gcgcagtgct gcgtatcggg ttgaccggtg gcatcggcgc cgggaaatcg
      241 gcgctgtccg ccacgttcgc ggaatgcggc gcgatcatcg tcgacggcga tgtcatcgcg
      301 cgtgaggtgg tgcaaccggg caccgaaggc ctggcgtcgc tggtcgatgc gttcggtgcc
      361 gacatcctgc tcgcggacgg atcattggac cggccggcct tggctgccaa ggcgtttcgg
      421 gacgacgagg cccgcggcgt gctcaacggg atcgtccacc cactggtggc caagcgccgc
      481 acggagatcg tcgctgcggt agcggaagat gccggggacg ttgttgtcgt cgaagacatt
      541 ccgctgctgg tggaatccgg gatggcgccg ttgttcccgc tggtcgtcgt ggtgcatgcc
      601 gacatcgacg tgcgggtgcg acggctggtc gagcaacgcg gcatgtctga ggacgacgcc
      661 cgcgccagga ttgccgcaca ggccgacgac gagcagcgtc gtgccgtcgc cgacgtctgg
      721 ctggacaact cgggcggccc aggggaattg gtggggcggg cccatgacgt gtggaacaac
      781 cggattctgc cgttcgccca caacctgagt gcgcggcagg ccgccagggc agcagcgcgg
      841 ctggtgccgc cggatccgag ttggcccgat caggcgcggc ggatcctggc ccgcctgaac
      901 accacctgcg gccaccgggc cgtgcggatc gaccacatcg gctcgaccgc ggtgccggca
      961 ttcccggatt tccaagccaa ggacgtcatc gacatccaga tcaccgtcga atcactcgcg
     1021 gtcgccaacg aactcgccgg accgctgttg tccgccggct acccgcgcat cgagcgcatc
     1081 acccaggatg tggccaaggc ccccagggtc gacgcccgca gcaccgtcga acgctatgac
     1141 cataacgacg atccgtcatt gtggcacaag cggattcacg catccgcaga tccgggtcga
     1201 ccgaccaacg tgcatatccg ggtggatggc tggcccaatc agcagttcgc cctgctgttc
     1261 gtcgactggc tggcggccaa ccccgacgtg cgcgccgact acctggccgt caagcgtgcg
     1321 gccgaacagc gcgcgcacgg ggacaccgct gactacgtcg ccgtcaagga gccgtggttt
     1381 ctcgacgcct acccgagggc gtggcagtgg gccgactcga ccgggtggcg gccctaggcg
     1441 ggttctgaaa cccgtcacgc tccgcgccag gtgagctcca tccctttgcc ggcgagccag
     1501 ccgtgcaggt catacccatt gcgggccagg ccgtcgacgg cggcaacggc acgctgcacc
     1561 gcccgttcgg cgtcttccgg gttcagcagg ccgtggcgga cggcgtccaa cagctcgtcg
     1621 acatcggcca gcgtcgcctc cgacccggtg cggacctcga tgtcgaggta atggtcgtcg
     1681 gcacgccaca cccgcgggcc gggagtgtat tcgcccacgt cgaggtagta gtcgtgatcg
     1741 cgttcgtggc ccgggttgaa gtggaagacg gtggcgcgca agcccaacga cggcagcagc
     1801 cacgattcga ggtaatggaa ctgcgcccgg ccgggcgtgg gacgggcgac atagagcccc
     1861 cacgggcgca ccgtgtactc ctcgacggcc cgcacaatgc ccttcggatc ggtattggtg
     1921 cgggcgacga ggtcgaacgt ctcatgcttc ggtggatgga tgatagtcag ccttccggcc
     1981 ctcaacggcc actcaacgtc ggagccggag tttccaccgc acaaagccgg catagaacac
     2041 cgacagcgcg gccagcatgc ccatgtcggt cagccagatc ttcgacgtgt gctgccagaa
     2101 ccggtcctgg ggaagcagcg aagccggaac caggtggcgt atgtcgaccg tcgacgccgc
     2161 cgcggcgtag ccccagcgcg cgggcattac gaaggacagc tggtcgaggc cgagccggcc
     2221 ggttaccggg accatcccgc cgcacagcac cagctgcgcc atgatcgaca ccacgaacag
     2281 cggcatgatc tgctcgctgg agcggaccag cgacgagatg gccagtccaa ggatcgccga
     2341 ggtgacacag gttccggcga cggtcaagaa cagctcgatc gtggccccga aagtgccgtg
     2401 acccagcagc accgccccct gggtcggcgc gcccttgccg atcaccacga tcgtggtggt
     2461 gatcgccgat tgcacaatgg cgaacccgca gtacaccatc gacttggcga gcagataggc
     2521 cgacgtcgac aaccccaccg cttgttcgcg ctggaagatg gcccgttcgc cgaccaggtc
     2581 tcggatcgtc agcgcggtgc ccatgaacgc ggcggcgagc agcagcaggt taagtatctg
     2641 cgcggattcg tcgggcgtac tggcgcccgg cccgggcacg ccgaatccac tggtgccggg
     2701 cacggtcagc gacagcacgc ccaggatgaa cggcagcacg gccaggaaga tgaagtaggc
     2761 gcggtcggcc accaccaacc ggacctgccg gcgggcgacc gtcgagatct ggcggcgcac
     2821 gctggtgtgt accggtttgc ccaggtcatc aggctcgtcg gtgctgtcgg gcagcagctt
     2881 ctggggtcgc ttctgggcat cccgctgggc gatgaaccgc ctgttggctt cttcggggtc
     2941 ggcgcccacc ttggtgaaga tcttggccca gttgctggtg cccatcgcgt caccgatctg
     3001 gtcgggcggg ccgcagtagg ccgtcttgcc gcccggtgcc accaaaagca gctggtcgca
     3061 gacgtcgagg taggacagca tgtgcgtcac gacgatcacc acgcgaccgg cgtcggccaa
     3121 ctggcgcagc atcgtcatga cctggtggtc cagcgccggg tccagacccg aggtcggctc
     3181 gtcgaggatc agcaatgagg gcccggtgag caattccaat gcgaccgaag cgcgtttgcg
     3241 ttgaccgccc gacagtttgt cgacgcgggt gtcggcgtgt ttggtcaggt cgagttcgcc
     3301 cagcacctgg gtgaccactt tggcccggtc ggctttgctg gtgtcgggcg gcagccgcag
     3361 ctcggcggcg tagcccaggg cctggttcac cgtcagctgg cggtgcacca cgtcgtcctg
     3421 cgggaccatg ccgatgcggc tgcgcaacga ggcgtagtcg gcgtggatgt cgtggccctc
     3481 gaaggtgacc gagccggaag tggggcgagc gtagccggcg atcagcctgg ccagcgtgct
     3541 ctttccggcg cccgacccgc cgatgaccgc ggtcagggtg ccgggcctgg cggacaggga
     3601 gatgttgtcc agcagttgtt tgccgttgtc gacgacgtac ttgacgttgc gcacctcgag
     3661 cccgcccgtg cgcgccgccg ccgcactgcc cgggaccagc gttccgtcgc ggaacacgag
     3721 gtcgacgttg ccgatggtga cgacgtcgtc gtcggtcagg atcgccgatc cgaccctggt
     3781 cccgttgacg aaggtgccgt tgacgctgtt gtcccggatc tcggtgccga gcggagtggg
     3841 caccaacgtc gcgtggtggc gtgaggccaa gacgtcggag acgacgaagt cgttgtcggc
     3901 ggcgcggccg atcgtcatgg cgcccgtcgt gccggcggcc gccgacgagc gcggcgtgag
     3961 tagcttcaca aaacgcgtcg ccaggttcga cacgtcggcc gtcttggcgt tgatgacggt
     4021 ggactcggcg gccggcgtgg actcgagctg ggccggcggc ccggtgtcgc ttccggccgc
     4081 tggcgtcggg tgcgaccggg ccaccgaacc ggtgttcacc acggtcacct gatcgggcgg
     4141 cgtcccaatg ggcggccggg ccggtgcggg catccccgac gtgtggattc gttgcggcgg
     4201 cgcctggcgc ggctgcctcg gcggcgg
//
LOCUS       DASSGQ010000374         2682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1304237,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000374 DASSGQ010000000
VERSION     DASSGQ010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304237"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /gene="hisS"
                     /locus_tag="VFI55_16480"
     CDS             <1..1034
                     /gene="hisS"
                     /locus_tag="VFI55_16480"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET7075661.1"
                     /translation="TLRPEGTAGVVRAVIEHGLDRGALPVKLCYAGPFFRYERPQAGR
                     YRQLQQVGVEAIGVDDPALDAEVIAVADAGFRSLGLDGFRLEITSLGDESCRPQYREL
                     LQDFLFALDLDDETRRRAEINPLRVLDDKRPEVRAMTAAAPVLLDHLSDAAKQHFDTV
                     LAHLDALGVPYIINPRMVRGLDYYTKTTFEFVHDGLGAQSGIGGGGRYDGLMHELGGQ
                     DLSGIGFGLGVDRTLLALRAEGKTVGETTRCDVFGVPLSEQAKLRLAVLSGELRASGL
                     RVDMAYGDRGLKGAMRAADRSGARIALVLGDRDIESGTVGVKDLATGEQVSVPLDSVV
                     SELLSRLSP"
     gene            1121..2482
                     /locus_tag="VFI55_16485"
     CDS             1121..2482
                     /locus_tag="VFI55_16485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HET7075662.1"
                     /translation="MSSSGRAEILEVFDALDADMDRVCGLSFEALTTPERLRILERLE
                     KIARRLPVPGHALLNQLVQQATPAELGGKLSHAVADRLRITRGEASRRVAEAADLGPR
                     QAITGAPLAPRLAATAAAQRHGRVGAGHVAVIRRFLEELPCWVDASTRRRAEADLAAA
                     ATGHRPEVVGKIADRLTCYLNPDGDFRDEDRARRRGITVGSQDGDGMSRISGWLTPEA
                     RATMEAVLAKLAAPGMCNPDDDTPVVDGAPGQDAVQRDTRSGAQRNHDGLNAALRAVL
                     ASGKLGQHNGLPASIILTTTLADLQAAAGKALTGGGTLLPMSDVIRLARHAHHYLAIF
                     DNGKALALYHTKRLASPGQRIVLYAKDRGCTAPGCDVAGYYCEVHHVKDWASTHCTDI
                     NQLTLACGPNHKLLDKGWTTRKNAHRDTEWIPPPHLDHGQPRVNTFHHPEKLLRADGE
                     DDP"
     gene            complement(2500..>2682)
                     /locus_tag="VFI55_16490"
     CDS             complement(2500..>2682)
                     /locus_tag="VFI55_16490"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306650.1"
                     /note="catalyzes the cleavage of carbon-halogen bonds in
                     aliphatic compounds forming a primary alcohol and a
                     halide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HET7075663.1"
                     /translation="PKLFVNAEPGAILTGRIRDYVRTWANLSEITIPGVHFIQEDSPD
                     EIGSAIAQFVRELRAN"
BASE COUNT          445 a          898 c          911 g          428 t
ORIGIN      
        1 tgacgctccg gccggagggg accgccgggg tggtgcgcgc ggtgatcgag cacggcctgg
       61 accgcggcgc gctgccggtg aaactctgtt acgcggggcc gtttttccgc tacgagcggc
      121 cgcaggccgg ccgctatcgc cagctgcaac aggtcggggt ggaggcgatc ggcgtcgacg
      181 acccggcgct ggacgccgag gtgatcgccg tggccgacgc cggattccgc tcgctgggcc
      241 tggacgggtt ccggctggag atcacctcgc tgggggacga gagctgccgc ccgcaatacc
      301 gggaactgtt gcaggacttc ctgtttgcgc tcgacctcga cgacgaaacc cggcgacgcg
      361 ccgagatcaa cccgctgcgg gtgctcgacg acaagcggcc cgaggtgcgg gcgatgacgg
      421 ccgccgcgcc ggtgctgctc gatcacctgt ccgatgccgc caagcagcac ttcgacaccg
      481 tgctggcgca cctggatgcg cttggcgtgc cgtacatcat caacccgcgg atggtgcgcg
      541 ggctggacta ctacaccaag accacgttcg agttcgtgca cgacggtttg ggcgcgcaat
      601 ccggcatcgg cggcggtggc cggtacgacg gcctgatgca cgagctcggc ggacaagacc
      661 tgtccggcat cggatttggg ttgggcgtgg accgcaccct gctggcgctg cgcgccgagg
      721 gcaagaccgt aggggagacg acccgctgcg acgtgtttgg ggtgccgctg agcgagcagg
      781 ccaagctgcg gctggcggtg ctgtccggtg agctgcgcgc ctccggtctc cgcgtggaca
      841 tggcctacgg tgaccgcgga ctcaagggcg cgatgcgcgc cgccgaccgt tctggtgccc
      901 gtatcgcatt ggtgttgggt gaccgcgata tcgagtccgg aacggtgggg gtcaaggacc
      961 tcgcgaccgg cgagcaggtg tccgtgccgt tggattcggt tgtctccgag ttgctttcgc
     1021 ggctcagccc ctagcctggc tgccgtaatc gcttgcgcca cttgagtatc ggtggtttcg
     1081 gcgggggatt tgtgtcggtg gccgcggata ttattcgaac atgagttcga gtggtcgtgc
     1141 ggagatctta gaggtcttcg acgcgctcga cgccgacatg gaccgcgtct gcgggttgtc
     1201 gtttgaggcg ttgaccaccc cggagcgatt gcgaatcctg gagcgcctgg aaaagatcgc
     1261 gcggcggctg ccggtaccgg gccatgcatt gctcaatcag ctggtgcagc aagccacccc
     1321 cgccgagttg ggcggcaaac tgtcccatgc cgtggccgac cggttacgca tcacccgtgg
     1381 cgaggccagc cggcgggtgg ccgaggccgc cgacttgggg ccgcgtcagg cgatcaccgg
     1441 tgcgccgttg gcgccacggc tggccgccac cgccgcggca caacgccacg gtcgggtcgg
     1501 cgccggccat gtggcggtca ttcgccggtt tttggaggag ttgccctgct gggtcgacgc
     1561 gtccacccgc cgacgcgccg aggccgatct ggccgccgcg gccaccgggc atcgccccga
     1621 ggtggtcggc aaaatcgccg accggctcac ctgttacctc aacccggacg gcgacttccg
     1681 cgacgaggac cgcgcccgtc gccgcggcat caccgtgggc agccaagacg gtgacggcat
     1741 gtcgcggatc agcggctggt taacccccga ggcccgcgcc accatggagg cggtgttggc
     1801 caagttggcc gccccgggca tgtgcaaccc cgacgacgac acaccggtag tcgacggggc
     1861 acctggccag gacgcggtgc agcgtgacac ccgcagcggt gcccagcgca atcacgacgg
     1921 gctcaacgcg gccctgcggg cggtcttggc cagcggaaag ctcggccagc acaacggatt
     1981 accggcatcg atcatcctga ccaccacact ggccgacctc caagccgcgg ccggcaaggc
     2041 gctgaccggc gggggcaccc tgctgccgat gagcgacgtc atccgtctgg cccgccacgc
     2101 ccatcactac ttggcgatct tcgataacgg caaagccctc gcgctctatc acaccaagcg
     2161 gttggcatcc cccggccagc gaatcgtcct ctatgcaaag gaccggggct gcacagcacc
     2221 gggctgcgat gtagccggct actactgcga ggtccaccat gtcaaggact gggccagcac
     2281 ccactgcacc gacatcaacc aactcaccct ggcctgtgga cccaaccaca aactcctcga
     2341 caaaggctgg accacccgca aaaacgccca ccgcgacacc gaatggatcc ccccacccca
     2401 cctggatcac ggccaaccac gagtcaacac attccatcac cccgaaaaac tcctgcgcgc
     2461 ggacggtgaa gacgatccgt gacgatcgaa accgtcccct taattggcgc gtaactctcg
     2521 gacgaactgc gctatggccg agccgatctc gtccgggctg tcttcctgga tgaaatgcac
     2581 tccgggaatg gtgatttcac tgaggttggc ccaggtgcgg acatagtcgc ggatgcggcc
     2641 ggtaaggatg gcgccgggtt cggcattgac gaacagcttg gg
//
LOCUS       DASSGQ010000375         2926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1213710,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000375 DASSGQ010000000
VERSION     DASSGQ010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213710"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFI55_16495"
     CDS             complement(<1..402)
                     /locus_tag="VFI55_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HET7075664.1"
                     /translation="MPPHAAEPHADEPRADADAGADADARPRVMLLGSGESSRELAVA
                     LRHLGAQVIAVDDYANAPAHRVADQSLILPMTDPDGLSDAIARLQPDFVVTATDKLSG
                     PARGALERFTAAEPDDQFTELVPNARAVRLTG"
     gene            complement(538..1194)
                     /locus_tag="VFI55_16500"
     CDS             complement(538..1194)
                     /locus_tag="VFI55_16500"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET7075665.1"
                     /translation="MTDYSFARPKELDPEYEHHGGFPEYGPASPGTGFGRFVAAMRRL
                     QDLAVSADTGAGVWEEAADRALALVELLAPFQADEGKAPAGRTPDLPGMGSLLLPPWT
                     LARYGPDGVEMTGYFTRFHVGGNHAVHGGVLPLLFDHVFGMVSHAAGRPISRTAFLHV
                     DYRNVTPIDTPLLVRGRVTSTEGRKAFVAAELVDPGGRGDTVLAEANGLMVRLLPGQP
                     "
     gene            complement(1191..2489)
                     /locus_tag="VFI55_16505"
     CDS             complement(1191..2489)
                     /locus_tag="VFI55_16505"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET7075666.1"
                     /translation="MPAIVLIGAQWGDEGKGKATDLLGGRVQWVVRYQGGNNAGHTVV
                     LPTGENFALHLIPSGVLTPGVTNVIGNGVVVDPGVLLSELHSLEDRGVDTTKLLISAD
                     AHLLLPYHVAIDKVTERYMGSKKIGTTGRGIGPCYQDKIARMGIRVADVLDPQLLTHK
                     IEAALELKNQILVKIYNRKALEPHQLVETLLAQADGFRHRIADTRLLLNNALEAGETV
                     LLEGSQGTLLDVDHGTYPYVTSSNPTAGGAAVGSGIGPTRITTVLGILKAYTTRVGSG
                     PFPTELFDANGEYLSKTGGEFGVTTGRRRRCGWFDAVIARYATRVNGITDYFLTKLDV
                     LSSLETVPVCVGYRIDGSRTRDMPMTQSDLQRAEPIYEELPGWWEDISAAREFDDLPA
                     KARDYVLRLEELAGAEVSCIGVGPGRDQTIVRRDVLAARP"
     gene            2671..>2926
                     /locus_tag="VFI55_16510"
     CDS             2671..>2926
                     /locus_tag="VFI55_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50"
                     /protein_id="HET7075667.1"
                     /translation="MKACGNTGADIPTVAVLVFGDRWAQSSLAGLPTHRAETPADVDA
                     AISGCRRLVVVGADADLATVLTRLLRTDRLDIEVAYAPPRR"
BASE COUNT          447 a          993 c         1002 g          484 t
ORIGIN      
        1 tccggtgagc cggacggcgc gagcgttggg caccagttcg gtgaactggt cgtccggctc
       61 ggcggcggtg aagcgctcga gtgcaccgcg ggccgggccg gagagtttgt cggtggcggt
      121 caccacgaag tctggctgca gccgggcgat cgcatcagat agcccgtcgg ggtcggtcat
      181 cgggaggatc aacgactggt cggccactcg gtgcgccggc gcgttcgcgt agtcatcgac
      241 ggcgatcacc tgcgcgccga gatgccgcag ggccacagcc aactcccggc tcgactcacc
      301 ggaacccagc agcatcaccc ggggcctggc gtcggcgtca gcgccggcat cggcgtcagc
      361 gcgcggctcg tcggcgtgcg gctcggcggc gtgcgggggc acgacagtca ccgtcgtgtt
      421 gtcgtcttcg gcagccgccg gttgcccacc gggctcgtgc tgtccttcgg tcacgccgtc
      481 agtcaccgct caaggatagg tccgccgcgg gcggcggcga ccaagcgtct tgccacctca
      541 gggctggccg ggcagtagcc gaaccatcag gccgttggct tcggccaaca ccgtgtcgcc
      601 acgcccgccc gggtcaacca attccgcggc cacgaaagcc ttgcggcctt cggtgctggt
      661 cacacgccca cgcaccaaca acggtgtatc gatcggggtg acgttgcggt agtcgacatg
      721 caggaaagcc gtccggctga tcggccgtcc cgctgcatgc gagaccatcc cgaacacgtg
      781 gtcaaacagc agcggcagta caccgccgtg gaccgcgtgg ttgccgccca cgtgaaaccg
      841 cgtgaaatag cccgtcattt cgacgccgtc ggggccgtac cgtgccaacg tccacggcgg
      901 cagcagcagg ctgcccatgc cgggtaggtc gggcgtccgc cccgccggcg ccttgccctc
      961 gtcggcctgg aacggggcca gcagctccac cagcgccagg gcacggtcag ccgcttcttc
     1021 ccataccccg gctccggtgt cagcggaaac cgcgagatcc tgcagccgac gcatggccgc
     1081 cacgaagcgg ccgaatcccg tgccggggct cgcggggccg tattccggaa agccgccgtg
     1141 gtgttcgtat tcggggtcga gttctttggg gcgcgcgaac gaataatccg tcacgggcgg
     1201 gccgccagga cgtcgcggcg cacgatggtc tggtcacgtc cgggaccgac accgatgcac
     1261 gaaacctccg ctccggcaag ctcttccagt cgcaacacgt agtcgcgcgc cttggcgggc
     1321 aagtcgtcga actcgcgggc cgccgagatg tcctcccacc agccgggcag ctcttcgtag
     1381 atgggttcgg cgcgctggag atcgctttgc gtcatcggca tatcgcgggt ccgcgacccg
     1441 tcgatccggt agccgacgca gacgggcacc gtttccaggc tggacagcac atccagcttg
     1501 gtcaggaagt agtcagtgat gccgttgacc cgggtggcgt agcgggcgat gacggcgtcg
     1561 aaccaaccgc agcgccgccg ccgtccggtg gtgacgccga attcgccgcc ggtcttggac
     1621 aggtactcgc cgttcgcgtc gaacagctcg gtggggaacg ggccggaacc cacgcgagtg
     1681 gtgtaggcct tgaggattcc cagcacggtg gtgatccgcg tcggtccgat cccggacccg
     1741 acggccgcgc cgcccgccgt tggattcgac gaggtcacat agggatacgt gccgtggtcg
     1801 acgtcgagca gggtgccctg cgagccttcc agcaagaccg tttcgccggc ctccagcgcg
     1861 ttgttgagca gcagccgggt gtcggcgatg cggtgccgga agccgtccgc ctgcgccaac
     1921 agcgtctcga ccagctggtg gggctccagc gcctttcggt tgtagatctt gaccaggatc
     1981 tggttcttga gttccagcgc cgcctcgatc ttgtgggtca acagctgcgg gtcgaggacg
     2041 tcggcgaccc gaatgcccat gcgcgcgatc ttgtcctggt agcagggccc gatcccgcgg
     2101 ccggtggtgc cgatcttctt gctgcccata taccgctcgg tgaccttgtc gatggcgacg
     2161 tggtagggca acagcaggtg ggcgtcggcg gagatcagca gcttggtggt gtcgacgccg
     2221 cggtcttcca ggctgtgcag ttcgctgagc agcacacccg gatccacgac cacgccgttg
     2281 ccgatgacgt tggtgacacc gggcgtcagc actcccgacg ggatcaggtg cagcgcgaag
     2341 ttttcgccag tgggtaacac gaccgtgtgc ccggcgttgt tcccgccctg ataccgcacg
     2401 acccactgca cgcgcccacc aagcaggtca gtggccttac ctttgccctc gtcgccccac
     2461 tgggcgccga tgaggacgat tgccggcatg actttgctcc cgcctaatcc cacgagttga
     2521 tgcctgttta ttgtggtcca gccggtgccc caccttagcc cagcgacgat gcgggccgcc
     2581 cgaggcccgc tgtgggggtc cccccagccg cgcagcggca agggggagag ccgggcaact
     2641 cagcttaggc caggaggttg cgaggaaccc atgaaagcct gcggcaacac tggcgcggac
     2701 atcccgactg tggcggtgtt ggtgttcggt gatcggtggg cgcaatcgtc attggccggc
     2761 ctgcccaccc accgagccga gaccccagcc gatgtcgacg cggcgatcag cgggtgccga
     2821 cgcctggtcg tcgtgggcgc cgatgccgac ctggccaccg tgctgacccg gttactgcgc
     2881 accgaccggc tcgacatcga ggtggcgtat gcgccgcccc gccgca
//
LOCUS       DASSGQ010000376         9984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_667232,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000376 DASSGQ010000000
VERSION     DASSGQ010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9984
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667232"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFI55_16515"
     CDS             complement(<1..527)
                     /locus_tag="VFI55_16515"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HET7075668.1"
                     /translation="MMTKLLVASRNPKKLAELRRVLDGAGLSGLTLVSLNDVAPFDEA
                     PETGATFEDNALAKARDAFAATGLASVADDSGLQVAALGGMPGVLSARWSGAHGDDAG
                     NTALLLAQMRDVPDERRGAAFVSACALVSSAGEVVVRGEWPGTIAREPRGDGGFGYDP
                     IFVPDGPDVGPRTAA"
     gene            694..1137
                     /locus_tag="VFI55_16520"
     CDS             694..1137
                     /locus_tag="VFI55_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET7075669.1"
                     /translation="MRQRPDTETLKALNNNITDEFRTNAGKVGGRFEGNELLLLTTKG
                     AKSGEPRVAPLVVFRIDGKLLIVAGYGGADVNPAWVHNLRANPRARVEVATDSFDVVA
                     HELDSFEREAIIPKINTTVPAFAFANYQSKTTRTIPIFELQKDES"
     gene            complement(1231..2010)
                     /gene="rph"
                     /locus_tag="VFI55_16525"
     CDS             complement(1231..2010)
                     /gene="rph"
                     /locus_tag="VFI55_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET7075670.1"
                     /translation="MSKREDGRLDDELRPVVITRGFTDHPAGSVLIEFGHTKVMCTAS
                     VTEGVPRWRRGTGLGWLTAEYAMLPSATHTRSDRESVKGRPSGRTQEISRLIGRSLRA
                     CIDLAALGENTIAVDCDVLQADGGTRTAAVTGAYVALADAVTYLSAAGKLSDPRPLSC
                     AIAAVSVGVVDGRVRVDLPYDEDSRAEVDMNVVATDTGTLVEIQGTGEGATFPRSTLD
                     KLLDMALGACDELFAAQREALELPYPGVLPSGPLPPKAFGS"
     gene            complement(2045..2827)
                     /locus_tag="VFI55_16530"
     CDS             complement(2045..2827)
                     /locus_tag="VFI55_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-degrading phosphodiesterase"
                     /protein_id="HET7075671.1"
                     /translation="MSVRITVLGCSGSVVGPDSPASGYLLRAPDTPPLVLDFGGGVLG
                     ALQRYADPGSVHVLLSHLHADHCLDMPGLFVWRRYHPSRPLGKSLLYGPSDTWSRLGA
                     ASSPFGGEIDDCSDIFDVHHWVDGEPVTFGGLTVVPRVVAHPTESYGLRITDASGASF
                     VYSGDTGICDELVELAHDADVFLCEASWTHGPDRPHALHLSGAEAGRVAAQAGVHELL
                     LTHIPPWTSREDVISEAKAEFDGPLHAVVCGETFDIRRSERV"
     gene            complement(2902..3720)
                     /gene="murI"
                     /locus_tag="VFI55_16535"
     CDS             complement(2902..3720)
                     /gene="murI"
                     /locus_tag="VFI55_16535"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET7075672.1"
                     /translation="MTSPLAPVGVFDSGVGGLTVARSIIDQLPDEDLIYVGDTANGPY
                     GPLTIPEVRAHALAIGDDLVDRGVKALVIACNTASAACLRDARERYDVPVVEVILPAV
                     RRAVVTTRSGRIGVIGTRATINSHAYQDAFAAARDTEITAVACPRFVDFVERGVTSGR
                     QVLGLAEGYLEPLQRAQVDTLVLGCTHYPLLSGLIQLAMGENVTLVSSAEETAKEVVR
                     VLTERDLLRPHDAPPATRVFEATGDPEEFTKLAARFLGPAVTGVQPIHRQSRVY"
     gene            complement(3717..4382)
                     /locus_tag="VFI55_16540"
     CDS             complement(3717..4382)
                     /locus_tag="VFI55_16540"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET7075673.1"
                     /translation="MTSGYPHTPTGQAKKRPRWLVGGATILTFVALLYLVELIDQLTG
                     HSLDANGIRPLESDGLWGIVFGPILHANWQHLMANTIPLLVLGFLMTLAGLSRFVWAT
                     AIVWILGGFGTWLIGDIGSNCGPTDHIGASGLIFGWLAFLMVFGIFVRRFWDIVIGLA
                     VLVVYGGVLLGAMPVLGRCGGVSWQGHLCGAIAGVVAAYLLSAPERKARAQRKALPPP
                     LKT"
     gene            complement(4565..5608)
                     /locus_tag="VFI55_16545"
     CDS             complement(4565..5608)
                     /locus_tag="VFI55_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET7075674.1"
                     /translation="MNSITDVGGIRVGHYQKLDPDASLGAGWACGVTVVLAPPGTVGG
                     VDCRGGAPGTRETDLLDPANTVRAVDAVLLAGGSAYGLAAADGVMRWLEEHGRGVGMD
                     GGVVPIVPGAVIFDLPVGGWDCRPTAEFGYAACVAADQATDGEAMAVGTVGAGVGARA
                     GVLKGGIGTASVVLPSGVSVGAVVAVNSAGDVVDRTTGLPWMADLIEAFGLTRPPDEQ
                     IDAFAKLPSPLSPLNTTIAVVATDAALSPAGCRRVAIAAHDGLARTIRPPHTPLDGDA
                     VFALATGAVEVAPGGGSNTMPAAFSPETGLVTEIGIAAADCLARAVLVGVLAAESVAA
                     IPTYRQMLPGAFG"
     gene            complement(5625..6206)
                     /locus_tag="VFI55_16550"
     CDS             complement(5625..6206)
                     /locus_tag="VFI55_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 domain-containing protein"
                     /protein_id="HET7075675.1"
                     /translation="MRKWKRVETASGPRFRSALAPHEAALLKNLVSAMIGLLDERESS
                     GPSDELEVITGIKTGNAEPPNDPTLRRLLPDFYRPDGDDSLDGNISDSLNAALRSLHE
                     PEIIDAKRVAAQQVLDTVPDHGGRFELTEADANAWVAAVNDVRLTLGVMLEIGPEGPD
                     RLPAGHPLAVHFDVYQWLTVLQEYLVLVLMGPR"
     gene            complement(6232..6537)
                     /gene="clpS"
                     /locus_tag="VFI55_16555"
     CDS             complement(6232..6537)
                     /gene="clpS"
                     /locus_tag="VFI55_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HET7075676.1"
                     /translation="MVVASAPTKPGSTGQRESAPVDAAVSPWVTIVWDDPVNLMTYVT
                     YVFQKLFGYSEPHATKLMLQVHNEGRAVVSAGSRETMEVDVSKLHAAGLWATLQQDR"
     gene            6593..7918
                     /locus_tag="VFI55_16560"
     CDS             6593..7918
                     /locus_tag="VFI55_16560"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET7075677.1"
                     /translation="MSDPVLAGLLTDKYEFTMLAAALRDGTADRRATFELFARRLPDG
                     RRYGVVAGTGRLLEVLPQFRFDDDACQLLAEFLDTDTVRYLRDFRFAGDIDGYAEGEL
                     YFPGSPILSVSGSFAECVVLETLALSIFNHDTAVASAAARMVSAAGERPLIEMGSRRT
                     HERAAVAAARAAYIAGFAASSNLEAQRRYGIPTEGTSAHAFIMLHTRRDDPPEITELA
                     AFRAQVDALGAHTTLLVDTYDVTTGVANAVTAAGAALGAVRIDSGELGVLARQVREQL
                     DGLGATQTRIVVSGDLDEFSIAALRADPVDSYGVGTSLVTGSGAPTANMVYKLVEVDG
                     MPVQKRSTHKESHGGRKAALRLSRPTGTITEEVVYPAGRPPATTEPSRVLTTPLVRGG
                     RVIGHEAHVGRCDLAAARKLVASGLRCLPWEGLNLSHGDPAIPTTQITA"
     gene            7971..9980
                     /locus_tag="VFI55_16565"
     CDS             7971..9980
                     /locus_tag="VFI55_16565"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HET7075678.1"
                     /translation="MSVSVHELLAIAVAALGGSERSGQQQMAAAVAQAFETGEHLVVQ
                     AGTGTGKSLAYLIPAIVRSLSGGSPVVVSTATIALQRQLVDRDLPRLADSLADALPRS
                     PRFALLKGRRNYLCLNKIHNGSAADSDDGVGGDRPQEELFDAMAATALGRDVQRLTVW
                     AATTDSGDRDDLKPGVADRSWSQVSVSARECIGVARCAFGSECFSERARGEAGRADVV
                     VTNHALLAIDAVAEAAVLPEHELLVVDEAHELVDRVTSVATAELTPAALGAVARRITR
                     LVDPELTQRLDATTANFAAAIHDAQIIKPGRIDRLDDEMATYLTALRDVASAARSAID
                     TTNDAKAASARAEAVAALSEISDTASRILTSFAPAIPDRTDVVWLEHEDNRGSPRPVL
                     RVAPLAVAGLLRNQVFARSTTVLTSATLTIGGSFDAMAAAWGLAGPDSAEYPPWRGLD
                     VGSPFHHAKAGILYVAAHLPPPGRDGTGSAEQLTEIAELITAAGGRTLGLFSSMRAAR
                     LAAEAMRERLSTPVLCQGDDGTSALVEQFSADAETSLFGTLSLWQGVDVPGPSLSLVL
                     IDRIPFPRPDDPLLGARQRAVAARGGNGFMTVAAAHAALLLAQGSGRLLRRVTDRGVV
                     AVLDSRMVTAGYGRYLRASLPPFWQTTDAAQVRGALQRLRAATEA"
BASE COUNT         1717 a         3493 c         3264 g         1510 t
ORIGIN      
        1 tgcgccgcgg tgcggggccc gacgtcaggg ccgtccggaa cgaagatcgg gtcgtagccg
       61 aatccgccgt caccgcgcgg ctcccgggca atggtgccgg gccactcccc gcgcacgacg
      121 acttcgcccg cgctcgaaac cagtgcgcag gcggacacga acgccgcgcc gcgccgttcg
      181 tcgggcacgt cacgcatctg cgccaacagc agcgcggtgt tgccggcgtc gtcgccgtgg
      241 gcacccgacc agcgcgcgga cagcacgccg ggcatgccgc ccagcgcggc cacctgcaga
      301 ccggagtcgt cggccacact ggccagacct gtcgcggcga acgcatccct ggccttggcc
      361 aacgcgttgt cctcgaaagt cgcgccggtc tccggcgctt cgtcgaatgg cgcgacgtcg
      421 ttcagcgaaa ccagcgtcaa gcccgacagc ccggctccgt ccagcacgcg gcgtagttcg
      481 gccagcttct tggggttgcg gctggcgacc agcagcttgg tcatcatcta ttcctaaaca
      541 tgtgtctggg ccgttgccga tgcaccccgg ccaccttacg gacggcgaga cgctgggtcg
      601 gcgacgcgaa tcgcattaag cagtgccggc acgatgtcca acgcggcacg ctccgcaagg
      661 aatagacagg gcgacgaagt agcttcgtcc agagtgcgcc aaagacccga taccgagacg
      721 ctcaaggcgc tcaataacaa catcaccgac gagtttcgca ccaacgccgg caaggtcggc
      781 gggcgattcg aaggcaacga gctgttgttg ctcactacga agggcgcgaa gtccggcgag
      841 ccgcgcgtcg cgccgctggt ggtcttccgt atcgacggca agctgctgat cgtcgcgggt
      901 tacggtggcg ccgacgtcaa cccggcgtgg gtacacaacc tgcgggccaa cccgcgggcg
      961 cgcgttgaag tcgccaccga ctcgttcgac gtcgtcgcgc acgagctcga ctcgttcgaa
     1021 cgtgaggcga tcatccccaa gatcaacacg accgtacccg cgttcgcgtt cgcgaactac
     1081 cagtcaaaaa ccacacgcac catcccaata ttcgagttgc agaaggacga gtcatgaccc
     1141 accggaaggg gtctcgtctt ggccgcggtg tcggcggtgg taaaccacgc ggcagttagg
     1201 ccaaggtcag ggtagcgacc agcggcgggg tcagctgccg aacgccttcg gcggtagcgg
     1261 tccgctgggc agcacacccg ggtacggcaa ctccagcgcc tcgcgctgtg cggcgaacaa
     1321 ctcgtcgcag gcacccagcg ccatgtcgag cagcttgtcc agcgtcgagc gcgggaacgt
     1381 cgcgccctcg ccggtcccct ggatctccac cagggtcccg gtgtcggtcg ccacgacgtt
     1441 catgtcgacc tcagcgcgcg agtcttcgtc gtagggcagg tccacccgga ccctgccgtc
     1501 gacgacaccg acgctgaccg ccgcgatcgc acaggacagg ggccgtggat ccgaaagctt
     1561 gcccgccgcc gacaggtagg tgaccgcgtc ggccaacgcg acgtaggcgc cggtgaccgc
     1621 cgcggtacgg gtgccgccgt cagcctgcaa cacatcgcag tcgactgcga tcgtgttctc
     1681 ccccagcgcc gccaggtcga tgcaagcccg cagcgaccgg ccgatcaggc ggctgatctc
     1741 ctgggtacgt ccgctgggcc ggcctttcac cgattcgcgg tcagagcgag tgtgggtggc
     1801 cgatggcagc atcgcgtact ccgcggtcag ccaccccaga cccgtgcccc tacgccagcg
     1861 tggtacccct tcggtaacgc tggcggtgca catgaccttg gtgtgaccga attcgatcag
     1921 taccgaaccc gccgggtggt cggtgaaacc gcgggtgatg actactgggc gaagctcgtc
     1981 gtcaagtcgg ccgtcttctc gtttggacac ccagtaaccc taagccaacc ctcgcccgag
     2041 gggctcagac cctttcagag cggcggatat cgaacgtctc accgcacacc accgcgtgta
     2101 ggggaccatc gaactcggcc ttggcctcgc tgatcacgtc ctcacgcgaa gtccacggcg
     2161 ggatgtgcgt cagcagcagc tcgtgaacac ccgcttgcgc cgcaaccctg ccggcctccg
     2221 cacccgacag atgcagcgcg tggggacggt ccggcccgtg tgtccaggag gcctcacaca
     2281 ggaaaacgtc ggcgtcgtga gccagctcga cgagctcatc gcagatcccg gtgtcaccgc
     2341 tgtagacaaa cgaggcaccg ctggcatcgg tgatcctcag gccgtaagac tcggttgggt
     2401 gggccaccac ccgtggcacc accgtgagcc caccgaacgt caccggctca ccgtcgaccc
     2461 agtgatgaac gtcgaagatg tccgagcaat cgtcgatctc cccaccgaag ggcgacgacg
     2521 cggcccccaa ccgagaccag gtatcgctcg ggccatagag caacgacttt ccgagcgggc
     2581 gcgacggatg gtagcgacgc cacacgaaca ggcccggcat atccagacag tggtcggcgt
     2641 gcaggtgaga caacaacaca tgcacggagc ccgggtcggc ataccgctgc agcgcaccaa
     2701 ggacaccccc gccgaagtca agtaccaaag gcggagtatc cggcgcccga agcagataac
     2761 ccgatgcagg cgaatccggc cccacgacgc taccggagca gccgagcacg gttattcgca
     2821 cggacactac tgtgccatgc ccgataccac gatggagaaa acatcgccgc attggtgtga
     2881 tgagaatctg gtcgcgcgca cctagtagac acgagattga cgatggattg gttgcacacc
     2941 ggtgaccgcg ggacccagga accgcgccgc cagtttggtg aactcctcgg ggtcgccggt
     3001 ggcttcgaac actcgggtgg cgggcggcgc gtcatgcgga cgcagcagat cccgttcggt
     3061 gagcacccga accacttcct tggcggtttc ctcggcgctc gaaaccaatg tcacgttctc
     3121 gcccatggcc agctggatca gcccggacag caatggatag tgcgtgcagc ccagcaccag
     3181 ggtgtcgacc tgggcgcgct gcagcggctc cagatacccc tcggccagtc cgagcacctg
     3241 acggccgctc gtcacgccgc gctcgacgaa gtcgacgaac cgcgggcagg ccaccgcggt
     3301 gatctcggtg tcgcgggcgg cggcgaacgc gtcctggtag gcgtgggagt tgatggtcgc
     3361 ccgcgtgccg atcaccccga tgcggccgct acgggtggtg acgacggcgc ggcgcaccgc
     3421 cggcaagatc acctcgacga cgggcacgtc gtagcgttcg cgagcatcgc gcaggcacgc
     3481 agcggacgcg gtgttgcagg cgatcaccag cgccttgaca ccacggtcga ccaggtcgtc
     3541 gccgatggcc agggcgtgcg cacgaacctc cgggatcgtg agcggtccgt aggggccgtt
     3601 ggcggtgtcg ccgacgtaga tgaggtcctc gtcgggcagt tggtcgatga tcgaccgcgc
     3661 gacggtcagt cccccgacgc cggagtcgaa aaccccgacg ggcgccagtg gcgaggtcat
     3721 gtcttcagtg gcggcggaag ggcttttctc tgagcgcgag ccttgcgttc gggggcggag
     3781 agcagatacg ccgccacgac gccggcaatg gcaccacata gatggccctg ccacgacacg
     3841 ccaccgcatc tgccgagcac cggcatggcg ccgagcaaga caccgccgta gacgaccagc
     3901 accgccagcc cgatgacgat gtcccagaat ctccgcacga aaatcccgaa aaccatcaag
     3961 aaagccagcc agccgaagat caggcccgag gctccgatgt ggtcggttgg accgcagttg
     4021 ctcccgatgt cgccgatcag ccaggtgccg aatccgccca gaatccacac gatcgcggtg
     4081 gcccagacaa atcgggacaa tccggccagc gtcatgagaa accccagcac cagcagcggg
     4141 atcgtgttgg ccatcagatg ctgccagttg gcatgcagaa tcggcccgaa gacgattccc
     4201 cacaggccgt cgctttccag cggcctgatg ccgttggcgt ccagggaatg cccggtcagc
     4261 tggtcgatca gctcaacgag gtagagcagc gccacgaagg tgaggatcgt ggccccgccc
     4321 accaaccagc gcggccgctt cttagcctgc ccggttggcg tatgcggata tcccgaggtc
     4381 atgagcgcag ctctaccgcg cgagcgggtg ttgcccccac ctcatcgcct cctctgtcgc
     4441 cgccgacgcg gatcacgtcg acagccgtcc ttctcagcgt ccacggcatc tttcaggctc
     4501 atcgtcaagg ctaccggcat acgcgccggt cgaggtcggc acggatcaac acctggacga
     4561 cccgtcagcc gaacgcgccc ggcaacatct ggcggtaggt cggtatcgcg gctaccgatt
     4621 cagcggcgag caccccgacc aacaccgcgc gtgccaggca atcggccgcg gctatcccta
     4681 tctcggtgac gagccccgtc tcgggcgaga aagcggcggg catcgtgttc gaaccgcccc
     4741 ctggagccac ctcgaccgcc ccggtagcca gtgcgaagac cgcatcgccg tccagcggag
     4801 tgtgtggggg ccggatggtg cgggccaggc cgtcgtgggc ggcgatcgcg acgcgccggc
     4861 accccgccgg gctcagcgcg gcgtcggtcg ccaccaccgc gatcgtggta ttcagcgggc
     4921 tcaacgggga cggcaatttc gcgaacgcgt cgatctgctc gtccggcggt cgcgtcaggc
     4981 cgaacgcttc gatcaagtcc gccatccacg gcagaccggt cgtccggtcg acgacgtcgc
     5041 cggccgagtt caccgcgacg accgcgccga cgctcacgcc cgacggcagg acgacagacg
     5101 ccgtcccgat accgcctttg agcacaccgg cccgcgctcc cactcccgcg ccaacggtcc
     5161 cgacggccat cgcttcgccg tcagtcgcct ggtcggcggc cacacacgcc gcgtagccga
     5221 attcggccgt cggacggcag tcccagccgc cgacgggcag atcgaagatc accgcgcccg
     5281 gcacgatggg caccacgccg ccatccatgc ccacaccacg cccgtgttct tccagccagc
     5341 gcatgacgcc gtccgcggct gccagaccgt aggcgctgcc gccggcaagt aacacggcgt
     5401 cgaccgctcg cacggtgttg gccggatcca gcagatcggt ctcccgggtg ccgggcgcac
     5461 ccccgcggca atcgacacca ccgacggtcc cgggcggagc cagcacgacg gtgacgccgc
     5521 acgcccaacc ggcgcctaac gacgcgtcgg ggtccagctt ctggtagtgg ccgacacgga
     5581 tacccccgac atcggtgatg gaattcatcc ggccgccatc gagatcatcg gggccccatc
     5641 aacaccaaga ccaggtattc ctgcaacacg gtcagccact ggtagacgtc gaagtgcacc
     5701 gccagcggat ggccggccgg caggcgatcc gggccctctg gcccgatctc gagcatgacc
     5761 cccaacgtca gccgaacgtc gttgaccgcc gcgacccagg cgttcgcgtc cgcctcggtc
     5821 agctcgaacc ggccgccgtg gtccggcacg gtatccaata cctgctgtgc cgcaacgcgt
     5881 ttggcgtcga taatctccgg ctcgtgcaga ctccgcagcg cggcgttgag gctgtcggag
     5941 atattcccat ccaacgagtc gtcgccgtcc ggccgataga aatccggtag cagccgacgg
     6001 agcgtcggat cgttcggagg ttcggcattt ccggtcttta tgccggtgat cacttcgagt
     6061 tcgtccgacg gcccagagga ttcgcgttcg tcgagcaggc cgatcatcgc actgacgagg
     6121 tttttcagca gtgcagcctc atgaggagcc aaggcagacc gaaaacgggg accgctggcg
     6181 gtctcgacgc gcttccactt gcgcacaagg agtcccggac gccctcgaat ctcaccggtc
     6241 ctgctgcagg gtcgcccaca aaccggcggc atgcagcttg gacacgtcga cttccatggt
     6301 ttcccggctg cccgccgaca ccaccgccct gccttcgttg tgtacctgca gcatcagctt
     6361 cgtggcgtgc ggttcgctgt agccgaacaa cttctggaac acatacgtca cgtaggtcat
     6421 caggttgacg gggtcatccc acacgatggt gacccacgga ctgaccgcgg cgtctaccgg
     6481 ggcggactcg cgttgcccgg tactgcccgg cttggtgggc gctgacgcaa caaccatgct
     6541 ctacacgata ccgacggagg tacctgttgc cgccagccgt taccgttgcg gaatgagtga
     6601 cccggtcctg gctgggctgc tgaccgacaa atacgagttc acgatgttgg cggcggccct
     6661 gcgcgacggc accgcggacc ggcgggccac gttcgaactg ttcgcgcgca gactccccga
     6721 cggccgtcgt tacggagtgg tcgcgggaac gggccgactg ttggaagtgt tgccgcagtt
     6781 caggttcgac gatgacgcat gccaattgct ggccgaattc ctagacaccg acacggtgcg
     6841 atacttgcgc gacttccggt tcgccggcga catcgacggc tatgccgaag gcgagctgta
     6901 cttccccggg tcgcccattc tctcggtgag tggcagcttc gccgaatgtg tggtgctgga
     6961 aaccctggcg ttgtcgatct tcaaccacga cacggcggtc gcgtcggcag ccgcacggat
     7021 ggtcagcgcc gcaggggaac gcccgctgat cgagatgggg tcacgccgaa cccacgaacg
     7081 cgcggcggtc gcggcggccc gtgccgcgta catcgccggc ttcgccgcct cctccaatct
     7141 ggaagcccaa cggcggtacg ggatacccac cgagggcacg tccgcgcacg cgttcatcat
     7201 gttgcatacg cgccgcgatg atccgcctga gatcactgaa ctggcggctt ttcgagccca
     7261 ggtcgacgcg ctgggcgccc acaccacgct gttggtggac acctacgacg tgacgaccgg
     7321 ggtggccaat gccgtgaccg ctgccggcgc cgccctcggt gcggtccgca tcgactccgg
     7381 cgagttgggt gtgctggccc gccaagtgcg cgagcaactc gacggcctgg gagccaccca
     7441 aacccgcatc gtggtgtccg gcgatctgga cgagttctcc atcgccgcat tgcgtgccga
     7501 cccggtggac agctacggcg tcggcacgtc gctggtgacc ggctcgggcg ccccgaccgc
     7561 gaacatggtc tacaagctgg tcgaagtcga cggcatgccg gtacagaaac gcagcaccca
     7621 caaggaatcc cacggcggcc gcaaagcggc gctgcgcctg tcccgcccga ccggcaccat
     7681 caccgaagag gtggtgtacc cggcgggccg tccgcccgcg acgaccgaac catcgcgggt
     7741 gttgaccaca ccgctggtgc gcggcggccg ggtgataggc cacgaagccc acgtcggtcg
     7801 gtgcgatctc gccgcggcgc gcaagctggt cgcgtcgggg ttgcgctgcc tgccgtggga
     7861 gggattgaac ctgtcgcacg gggaccccgc gatcccgacc acacagatca ccgcctgacc
     7921 tatacggtcg acgcaccgga tgcacaacca agggagcgac cacgtccgac gtgtcagtgt
     7981 ccgtgcacga gctgctggcc atcgccgtgg ccgccctcgg cggcagcgag cgcagcgggc
     8041 agcagcagat ggcagccgcc gtcgcgcagg ctttcgaaac cggtgagcac ctcgtggtgc
     8101 aggccggcac cggtaccggc aagtcgctcg catacctgat tcccgcgatc gtccgctcgc
     8161 tcagcggcgg ctcgcccgtc gtggtgtcga ccgccacaat cgcgttgcag cgccaactcg
     8221 tcgatcgcga cctgcccagg ctggccgatt cgctggccga tgcgctgccc cgcagcccgc
     8281 gattcgcgct gctgaagggg cgacggaact acttgtgcct gaacaaaatc cacaacggca
     8341 gcgccgccga cagcgacgac ggagttggcg gcgacaggcc gcaggaggaa ctcttcgacg
     8401 cgatggcggc gactgccctg ggccgcgatg tgcaacggct cacggtgtgg gcggcgacaa
     8461 ccgactcggg tgaccgcgac gatctcaagc ccggcgtcgc ggaccgatcc tggtcccagg
     8521 tcagcgtttc cgcgcgggaa tgcatcggtg tagcccgatg cgcgtttggc tccgagtgct
     8581 tctccgaacg ggccaggggt gaggccggcc gtgctgatgt cgtcgtcacc aaccacgcgc
     8641 tgctggccat cgacgccgtc gccgaggcgg cggtgctgcc ggaacacgag ctgctggtgg
     8701 tcgacgaggc gcacgagttg gttgaccggg tgacgtcggt cgccaccgcg gagctgacgc
     8761 cggccgccct cggtgcggtg gcccggcgga tcacccgact ggtggatccg gaactcaccc
     8821 agcggctgga tgcgacgacc gccaacttcg ccgcggcgat ccatgacgct cagataatca
     8881 agcccgggcg catcgatcgc ctcgacgacg agatggcgac ttacctgaca gcgctgcgcg
     8941 acgtggcctc cgcggcacgc tcggcgatcg acaccacgaa tgacgctaag gcggcgtcgg
     9001 cacgcgccga agctgttgcg gcactgagcg agatatcaga caccgcatcg cggatcctga
     9061 cctcgttcgc ccccgccatc cccgaccgca ccgacgtggt ctggctcgag cacgaggaca
     9121 accggggttc acctcgcccg gtgttgcggg tggccccgct ggcggtcgcc ggcttgctgc
     9181 gcaaccaggt attcgcgcgt tcgacgacgg tgctgacatc ggcgaccctg acgatcggcg
     9241 gatccttcga cgcgatggcc gcggcctggg gcctggccgg accggactcc gctgagtacc
     9301 caccctggcg tggtctcgac gtcggctcac cgttccatca cgccaaggcg ggcatcctct
     9361 acgtggccgc gcacttgccg ccgccgggcc gggacggtac cggctcggcg gagcagctga
     9421 cggagatcgc cgagctcatc accgctgccg gcgggcgcac gctgggactg ttctcgtcga
     9481 tgcgcgccgc gcggctcgcg gccgaggcca tgcgcgaacg gttgtcgacg ccggtgctgt
     9541 gccaaggcga cgacggcacc tccgcgctgg tcgagcaatt cagtgctgac gccgagacct
     9601 cgctgttcgg aaccttgtca ctgtggcagg gcgtcgatgt gcccgggcca tccctgtccc
     9661 tggtcctgat cgaccgaatc ccgttccccc ggccggacga cccgctgttg ggcgcgcggc
     9721 agcgcgcggt ggccgcacgc ggcggcaacg gtttcatgac cgtcgccgcc gcccatgcag
     9781 cactattgct ggcgcagggg tcgggtcggc tgctgcgccg cgtcaccgac cgcggagtgg
     9841 tcgcggtgct cgactcccgg atggtcaccg ccggctacgg gcgctacctg cgagcctcgc
     9901 tgccgccgtt ctggcagacc accgacgccg cgcaggtccg cggggcgctg cagcggctgc
     9961 gcgccgcgac agaggcctga gcgg
//
LOCUS       DASSGQ010000377         7089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_879728,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000377 DASSGQ010000000
VERSION     DASSGQ010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7089
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879728"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VFI55_16570"
     CDS             complement(<1..464)
                     /locus_tag="VFI55_16570"
                     /inference="COORDINATES: protein motif:HMM:NF013127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HET7075679.1"
                     /translation="MSFVIAVPESVAAAASDLASIGSTIGAANAAALGPTLGVLPAAA
                     DEVSAAVTSLFTAHAHGYQALSAQAAAFHDQFVRLMNAGAGQYGAAEAANASPLQAAE
                     QNLLNAVNAPTQALVGRPLIGNGANGAAGTGANGGAGGILIGNGGAGGSGAA"
     gene            1153..2313
                     /locus_tag="VFI55_16575"
     CDS             1153..2313
                     /locus_tag="VFI55_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HET7075680.1"
                     /translation="MSYLAAAPEFLARAASDLSNVGSQLIAANAAATAPTTAMVPAAG
                     DEISAAITSLFAGQARAYQALNSQAAAFHAQFVQTLNSAGGSYAAAEAANGSLLQTAE
                     QDALAAINAPTNALLGRPLIGDGANGTAANPNGGAGGLLFGNGGAGFSQAGNPGVGGG
                     AGGGAGLIGNGGLGGAGGVGAAGGAGGRGGYTYGNGGGGGNGGAASRAGGLNAFTGGT
                     GGTGGVGGAAGLWGAGGSGGQGGGAGPNSATLSTGQTTPFVLNGGNGGDGGRGGWLHG
                     QGGAAGQGGTGSPSQTVSLTVSGTSTMNFTILNNTPGSNVAVGFLPGTNVFSVSPSSA
                     SPPPGGTAPVTVNFQPGFTSGTVTCFQVVGGTFVPFAGTAGHAGNAGANGLL"
     gene            complement(2541..3629)
                     /locus_tag="VFI55_16580"
     CDS             complement(2541..3629)
                     /locus_tag="VFI55_16580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7075681.1"
                     /translation="MMVAETSYRTAQVTGADSYWLADHLTSVMPRAVMTAKHNGIARL
                     VPDVDAFLEPWTTLGALAGRHRFNRMQLGTCVTDTGRRNPAVTAQAAATLHLMTRGRA
                     ILGIGTGERVGNEPYGVDWSKPVARFEEALATIRALWNSNGQLVNRDSEWFPLRNAIF
                     TLPPVKGSWPQIWIASHGPRMLRATGRFADGWFPVSFFSPERYADGLQQVQAAADNAN
                     RDPRSIIAANTQFVVTGRSSAEVEEALDSVPVRAFALNIPAWAWAEHGAQHPLGESFV
                     GFQDILPQTFDEQTALAHAGAAPKSLIKQYALTGTPGEIIDRLAVWRDHGVRYPVLWN
                     ISVVQPKLSRGLSSALPFMKILRGIRRL"
     gene            complement(3730..4516)
                     /locus_tag="VFI55_16585"
                     /pseudo
     CDS             complement(3730..4516)
                     /locus_tag="VFI55_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148214.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(4533..4606)
                     /locus_tag="VFI55_16590"
     tRNA            complement(4533..4606)
                     /locus_tag="VFI55_16590"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4572..4574),aa:Gly,seq:ccc)
     gene            4662..5234
                     /gene="dcd"
                     /locus_tag="VFI55_16595"
     CDS             4662..5234
                     /gene="dcd"
                     /locus_tag="VFI55_16595"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HET7075682.1"
                     /translation="MLLSDRDLRAEIAAGRLGIDPFDDTLVQPSSIDVRLDCMFRVFN
                     NTRYTHIDPAKQQDELTSLVEPVDGEPFVLHPGEFVLGSTLELFTLPDNLAGRLEGKS
                     SLGRLGLLTHSTAGFIDPGFSGHITLELSNVANLPITLWPGMKIGQLCILRLTSPAEH
                     PYGSSRVGSKYQGQRGPTPSRSYQNFIRST"
     gene            5367..>7089
                     /locus_tag="VFI55_16600"
     CDS             5367..>7089
                     /locus_tag="VFI55_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075683.1"
                     /translation="MAPASIHMVLLEGENADGVTVEVDEFAVRAADDSATASASDQVI
                     AAILGTREGAADAGLELSAVGVAWTDQLAAAALRDALTAHKLENVMLVSAFLAATALA
                     QSVGGAMGYERTAVLFVEPDTATLAIIETSDGSIADLYKEQIDAASYDDATAQLTGMI
                     VGLEELETQSGGLFLVGSGVDVAPIKPALQSATELFVSVPEEPETALARGAALASANA
                     PLFASSTAALAYAQDPGTGAVDPYALPEYLGVAGLPVGAQLGDDDLAYSAFPDEDAES
                     PTVLIERLFAPERAQPRRRPVLLIGSGLAVVGISAVLALEIALAIGIRTTVALRPTPS
                     QNLIVPTQQVPVEAVAPQPKLSMAQPMVAPRPVNLPAAPLPAAPPIPAAAAPVVPVPV
                     VAPPLAPIPMPPLHLPIPDPIASPPLMPHLAAPQPMVPQEPVHVPQPQSPWQGNGTMP
                     HSPPHQVPPAEGSSPRPISPGGEGGGHFGPPGSGPGGEGGGHFPAPGAGPGGPGGGEP
                     TGPGSFGGGGGPFGGGGPFGGGGHGGGGHGGGESTGPGSFGGGGGPFGGGGPFGGGGH
                     GGGESTGP"
BASE COUNT         1176 a         2360 c         2352 g         1201 t
ORIGIN      
        1 ctggccgcgc cggacccgcc ggccccgccg ttgccgatca atatcccgcc ggccccgccg
       61 ttagccccgg tccccgcggc cccattggcg ccgttgccaa tcagcgggcg ccccaccaac
      121 gcctgggtcg gcgcattcac agcgttcagc agattctgct cggcggcctg cagcggcgac
      181 gcgttggccg cctcggcggc cccatactgc cccgcgccgg cgttcatcag tcgcacaaac
      241 tggtcatgaa acgccgccgc ctgggcactc agcgcctgat acccgtgggc gtgcgcggtg
      301 aacagcgacg tgacagccgc cgacacctca tcggcggccg ccggtaacac ccccagcgtc
      361 gggcccaacg ccgccgcgtt ggctgcccca atcgtcgaac cgatactcgc caaatccgaa
      421 gccgccgccg ccacagactc cggcaccgcg atcacaaacg acatctaaga cttcccccta
      481 ggtctcgact gataagtcat accgacccag ggacgcaaaa cacaaccctg aatctgcgcc
      541 tcacattgga gcgcgaaggc cttcggcggg gaggtcagac cgccgccacg acctgccgcg
      601 aaggcgggcg aatcgtgtga aatcgcgttg agcggaacca cgatcgcgtt acgatccgca
      661 ccgacttggt tcgccgtcgg actagcgagc agccgatgac gggaatcgag tccgcgtatt
      721 cagcttggag atcggtaaga gcgcggcggg aactgtaata gccgaaaaat tgattgacca
      781 gaggatagaa aatattgtcg aacggatcat caacaagatg caggaaaaag ggggagcacc
      841 tttttgagtg attcaaagat cctcggtgtc tcgctgtact tgtgtccgta gagagaagct
      901 tcgctgaaac cggtgacgag gggattgctc gtcgatctcg caggcaaacc tacgcgaatc
      961 aacatagtgc gccccgaaac tgaccactcg gcatctcaac gcgctgagtg ttatcttccg
     1021 aattgctcga atgacccatc ccctcgcgct ggacgttccc aatctgggaa tccgcatacg
     1081 ctagacgttc ttcacgttca tccgataaat atctgatttg tcgatgtgtc gacggctcgg
     1141 tgggaggaag cgatgtcgta tctggcggcg gctccggagt ttctggcgcg agcggcatcg
     1201 gatctgtcta acgtcggctc gcagctgatc gcggcgaatg cggcggcgac ggccccgact
     1261 accgcaatgg tgcccgctgc cggcgatgag atatcagcgg ccatcacgtc gctgtttgcc
     1321 ggccaggcgc gggcctatca ggcgctcaac agccaggccg cggcgtttca tgcccagttt
     1381 gtgcagacgt taaacagcgc gggcggctcg tatgcggccg ctgaggccgc caacggctcg
     1441 ctgttgcaga ccgcggagca agacgctttg gcggcgatca acgcacccac caacgcgttg
     1501 ctcgggcgcc cactgatcgg tgacggtgcc aatgggacgg cagccaaccc caacggcggg
     1561 gccggcggat tgttgttcgg caacggcggc gccggcttct cgcaagcggg caaccccgga
     1621 gtgggcgggg gcgccggtgg cggcgcgggg ctgatcggca acggcggtct cggcggagcg
     1681 ggtggcgtcg gcgcagcggg cggagccggc ggtcgcggcg gctacacata cggaaacggc
     1741 gggggcggcg gcaacggcgg ggcggcctcg cgggccggtg gcttaaacgc ttttaccggc
     1801 ggtaccggcg gtaccggcgg agtcggtggg gccgcggggt tgtggggcgc tggcgggagc
     1861 ggcgggcagg gcggcggcgc cggacccaac agtgcgacgc tgtccaccgg ccagaccacc
     1921 cccttcgtcc tgaacggcgg caatggcggc gatggcgggc gcggcgggtg gctacacggt
     1981 cagggtgggg ccgccgggca aggggggacc ggcagcccat ctcaaaccgt gagcctcacc
     2041 gtcagcggga cctccacgat gaacttcacg atcctcaaca acacccccgg aagcaatgtc
     2101 gccgtcggat tccttccggg caccaatgtc ttctccgtga gtccatcgag cgcgagcccg
     2161 cctcccggcg gcacggcccc agtgacggtc aacttccagc ccggctttac cagcggtacc
     2221 gtcacttgct ttcaagtagt aggcggtact tttgtgcctt ttgcggggac cgcgggccat
     2281 gccggcaacg ccggggccaa cgggttgctg tagagccaaa ccagggcgac cagattgggc
     2341 ccacggcgac gcgatggccg cgctcggggc aatgaggagc cctatgcaga gcgacaacgt
     2401 aattcagttg cacgggtagg tttgctgctg tgctcggtgt gtgcgtgcct agtttgtgga
     2461 cggccagagt acccatgcgc cgacgttgag gcggcccagc aaacgtgcaa tccacttggc
     2521 ccgagcgagg ttcggtgacc tcacagccgc cggattccgc ggaggatctt catgaagggc
     2581 aaggcgctgg acaggccgcg gctgagtttg ggttggacga cgctgatgtt ccacagcacg
     2641 gggtagcgca cgccatggtc gcgccacacg gctagccggt cgatgatctc gccgggcgtt
     2701 ccagtgagag catattgctt gatcagggat tttggcgccg cgccggcatg tgccagcgcg
     2761 gtctgttcat cgaacgtttg cggcagaatg tcctgaaagc cgacgaagct ctcgccgagg
     2821 gggtgctggg cgccgtgctc ggcccacgcc caggccggaa tattgagcgc gaaagcccgg
     2881 accggcaccg agtccaaggc ttcttcgact tctgctgaac tgcgtccggt cacgacgaac
     2941 tgcgtgttcg ccgcgatgat cgagcgagga tcccggtttg cgttgtcggc ggccgcctga
     3001 acctgctgca aaccgtctgc gtatcgttcc gggctgaaaa acgacaccgg gaaccaccca
     3061 tcggcaaagc ggcccgtcgc acgcagcatc cgcggtccgt gcgaggcgat ccagatctgc
     3121 ggccagctac ctttcaccgg gggcagggtg aaaatcgcat tgcgcagggg gaaccattcc
     3181 gaatctcggt tcaccagctg tccgttcgag ttccacagcg cgcggatcgt tgctagcgcc
     3241 tcctcgaacc tggccaccgg cttggaccaa tccaccccgt acggctcatt gcctacccgc
     3301 tccccggttc cgatcccgag gatcgcccgg cctcgggtca tcaggtgcag tgtcgccgcg
     3361 gcctgcgccg tcacggccgg gttccggcgg ccggtgtcgg tgacgcaggt gcccaactgc
     3421 atccggttga atcggtggcg gccagcaagg gccccgaggg tcgtccaagg ctcaaggaat
     3481 gcgtccacat cgggcaccag gcgggctatc ccgttgtgct tcgctgtcat caccgcccgc
     3541 ggcattacgc tggtgaggtg gtcggccagc cagtacgaat cagcaccggt cacctgagcc
     3601 gtccggtagc tcgtctcggc caccatcatc ggccccaacc ggctgtgcat tggtagttct
     3661 gcgacaccaa acttcactgc gacgtacccc tagattgcgt ggcggaagta tcgcatggaa
     3721 cccgtgtatt caccccaggg tgcgttggta gcggcgcatc ccagcgatcc acccgctcat
     3781 agtcggctcc cttctgccga tacatggtca gcacctcagg gtgcggcagc atcaggaacg
     3841 tcccgtcggc gatggcctga accacgaggg cggccacggt gtctgggccg atcaccgcac
     3901 cggcaagggc ggcggcggcc gcccgaaact ctgcgtccgg ggattcggtc atgccgcgca
     3961 gcagcggggt ttccacaccc atcgggcata cgcagctcac accgattccc ttgtcgccgt
     4021 atttgatggc cagccattcg gcgaacccga cggcggcgtg cttggtcacg ctgtagggcg
     4081 ccccaccgag ttgggtcagc aggccggccg ccgaggcgac ggcgacgaaa tgtccgctgg
     4141 cacgctcaag ccattccggc acaacagcct tggctgcgcg gacgtgggcg cgcaggttga
     4201 tgtcgatgat ccggtcccag gcgccttcgt cgtcgccgag gccggattgg ccggcgatgc
     4261 cggcattggc gacgtagatg tccaccgggc cgaacgttcg gcgtgcagtg tcgagcaggg
     4321 tcgcgatgcc gtccgtgctc gcggcgtctg cggacaccgc gacgcccccg gtcatcgcag
     4381 cggtctcggc agccgaacct gcgtcgatgt cgccggccac caccgaggcg cccgccgatg
     4441 ccagccccac cgcaatggac ctgccgatgc cggagcccgc tccggtgacc accgccaccg
     4501 atccgtcgat cttcacgggc acatggtcag cgtggagccg atgacgggaa tcgaacccgc
     4561 gtattcagct tgggaagctg atgttctgcc attgaactac atcggcgcgg ttgcctggag
     4621 aggctagcac ccgccgtcgt gccagcagat aggctcagcg ggtgctgctc tccgatcgcg
     4681 acctccgggc cgaaatcgcc gccggccggt tgggcatcga cccgttcgac gacaccttgg
     4741 tgcagccgtc cagcatcgac gttcgcctcg actgcatgtt ccgggtgttc aacaacaccc
     4801 gctacaccca catcgacccg gcaaagcagc aggacgagct gaccagcctg gtcgaacccg
     4861 tcgacgggga accgttcgtg ctgcatccgg gcgaattcgt gctgggctcg acgctggagc
     4921 tgttcaccct gcccgacaac ctcgcggggc gcctggaggg caagtcgtcg ctgggccgcc
     4981 tgggcctgct gacgcattcg acggccggct tcatcgatcc cggcttcagc ggtcacatca
     5041 cgctcgagct gtccaacgtc gccaacctgc cgatcaccct gtggccgggc atgaagatcg
     5101 gtcaactgtg cattttgcgg ttgaccagcc cggccgagca cccctacggc agttcgcgcg
     5161 tggggtcgaa gtaccagggt cagcgggggc cgaccccgtc gcggtcctat cagaacttca
     5221 taagaagtac ctaggttcgt cagcgcgctt gcacagcatt tctgcgggat gatcaattgg
     5281 tccgtggtta ctcctccgcc cgaccatggc gtggttggca atacctttgg agggggtttg
     5341 tattggatat cgtgctcggg gtttcgatgg caccggcgtc gatccacatg gtgctcctgg
     5401 agggcgaaaa cgctgacggc gtaacggtag aagtggacga gtttgcggtc cgcgctgctg
     5461 acgactcggc cactgcgagc gcgtccgatc aggtaatcgc cgccatcctg ggcactcggg
     5521 agggcgcggc cgatgccggc cttgagctgt cggctgtcgg ggtggcgtgg accgatcaac
     5581 tcgccgctgc cgctttgcgt gacgcgctga ccgcccacaa gctcgagaat gtgatgctgg
     5641 tctcagcgtt tctggccgcg acagcgctag cccaatccgt cggcggcgca atgggttacg
     5701 agcgcaccgc ggtgcttttc gtggagcctg acaccgcgac gctggccatc atcgagactt
     5761 cggacggttc catcgcggat ctctacaaag agcagatcga tgccgcgtcg tacgacgacg
     5821 ccaccgcgca actcaccggc atgatcgtcg ggctagagga gctggaaacg cagtcgggcg
     5881 gcctgttttt ggtgggctcg ggcgtcgacg tcgccccgat aaagccggcc ctgcagtctg
     5941 cgacggagct tttcgtgagc gttccggagg agccggagac cgcgctggcc cgcggcgccg
     6001 ccctggcatc ggcgaacgcg ccgttgttcg cctcgtcgac ggccgcgctg gcttacgctc
     6061 aagaccccgg aaccggtgcg gtcgacccat acgcactgcc cgaatacctc ggtgtggcag
     6121 ggcttcccgt tggtgcacag ctcggcgacg acgacctcgc ctacagcgct ttcccggacg
     6181 aggacgccga atcgccaacg gtcctcatag aaaggctctt cgctcccgaa cgagcccaac
     6241 cgcgtcgcag gccggtgcta ctgatcggca gcgggctggc ggtcgttggc atcagtgcgg
     6301 tgctggcgct ggaaattgcg ctggcgatcg gcatccgcac caccgtcgct ttgcggccaa
     6361 cccctagtca aaacctcatc gtcccaacgc aacaggtgcc ggtcgaggcg gtcgccccgc
     6421 agccgaagct cagcatggcg cagccgatgg tggcgcccag gccggtgaac ctgcccgcgg
     6481 ccccgttgcc cgccgcgcct ccgattcccg cggctgccgc tccggtggtg ccggttccgg
     6541 tggtggcccc tccgctggcg ccaattccga tgccaccgct ccacctgccg attcccgacc
     6601 cgatagcgag cccaccgctg atgccacacc tggcggcacc gcaacccatg gtgccccaag
     6661 agccggtgca tgtcccgcag ccgcagtcgc cgtggcaagg caacggcaca atgccacatt
     6721 cgccacccca ccaggttcct ccggcagaag ggtcgtctcc caggccgatc agccccggcg
     6781 gcgagggtgg cggacacttc gggccccccg gctcaggccc gggcggcgaa ggaggcggac
     6841 actttccggc cccgggcgcc ggtcccggcg gccccggcgg cggtgagccc accggacctg
     6901 gttcgtttgg cggtggcggc ggccccttcg gcggcggcgg accgttcggc ggtggtggtc
     6961 atggcggtgg tggtcatggc ggcggtgagt ccactggacc tggttcgttt ggcggtggcg
     7021 gcggcccctt cggcggcggc gggccgttcg gcggtggtgg tcatggcggc ggtgagtcca
     7081 ccggacctg
//
LOCUS       DASSGQ010000378        13698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_758361,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000378 DASSGQ010000000
VERSION     DASSGQ010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13698
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758361"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFI55_16605"
     CDS             <1..439
                     /locus_tag="VFI55_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET7075684.1"
                     /translation="MVICGKADVVHDDPAGPVIHSATRSIVVPSVIQLRSYVRVPYRA
                     RVPMTRAALMHRDRFCCAYCGAKADTVDHVVPRSRGGDHSWENCVACCSTCNHRKGDK
                     LLTELGWALRRVPLPPTGQHWRLLSTVKELDPAWARYLGEGAA"
     gene            519..755
                     /locus_tag="VFI55_16610"
     CDS             519..755
                     /locus_tag="VFI55_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075685.1"
                     /translation="MSLVEVHLYVLGIPLLLVIVLSVLIWSKKGPHPATYELSEKWTH
                     PPILWAAVDEDVGGAHGGHGTSEFSVGGGASGTW"
     gene            742..1209
                     /locus_tag="VFI55_16615"
     CDS             742..1209
                     /locus_tag="VFI55_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5130 domain-containing protein"
                     /protein_id="HET7075686.1"
                     /translation="MARGEVATIARSELPKGSVITTSGRISGVTEPGAVSSDYPFPIT
                     DLVAIDDALKYASRESGARFAIYLGDLGSDTAARAREIWAKVPTPDNAVLLAVSPNQS
                     AIEVVYGSAVRGRGAESAAPLGVAAASSAFEQGHLVEGLVSAVVELSDNISRA"
     gene            complement(1297..3927)
                     /gene="pepN"
                     /locus_tag="VFI55_16620"
     CDS             complement(1297..3927)
                     /gene="pepN"
                     /locus_tag="VFI55_16620"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HET7075687.1"
                     /translation="MALPNLTRDQAVERAALITVDSYQINLDVTDGPDGSDDRSAPGE
                     RTFRSTTTVVFEALAGADTVIDIAADTVRSATLNGRELDVSGYDESTGIPLRGLADRN
                     VVVIDTDCSYSNTGEGLHRFVDPVDGETYLYSQFETADAKRMFACFDQPDLKATFNLR
                     VLAPKHWKVISNGATESIESVGDGENSAEKSVHTFATTPRMSTYLVALIAGPYAEWKD
                     SYVDEHGEIPLGIYCRASLAGHMDAERLFTQTKQGFGFYHNNFGLPYAFGKYDQLFVP
                     EFNAGAMENAGAVTFLEDYVFRSRVTRASYERRAETVLHEMAHMWFGDLVTMTWWDDL
                     WLNESFATFASVLCLSEATEFTEAWTTFANVEKSWAYRQDQLPSTHPIAAEIPDLAAV
                     EVNFDGITYAKGASVLKQLVAYVGLEQFLAGLRDYFRAHAFGNATFGDLLAALENASG
                     RDLSTWGQQWLKTTGLNTLRPDFDVDADGKFTRFAVTQSGATPGAGETRVHRLAVGIY
                     DDDGSGKLVRVHQVELDVDGPETKVPALVGVSRGKLILVNDDDLTYCSVRLDPHSLQT
                     ALARIADIAEPLPRTLAWSAAWEMTREAELRARDFVALVSGGVHAETEVGVAQRLLLQ
                     AQTALGSYAEPAWAREHGWPQFADRLVELARAAQPGSDHQLAFVNTLCSSVLSPRHVK
                     TLAALANSDPAGLGLAGLEIDTDLRWRIVTALATAGNIDADGPATPFIDAEVQRDPTA
                     AGKRHGAQAAAARPQLQVKEDAWTSVVEDDALPNITARAMIAGIAAPGQGELLQPFVA
                     RYFAAIPRVWARRSSEVAQTVVFGLYPHWDISDAGVAAADEFLTAPESEVPPALRRLV
                     LEGQAGVKRALRARAFDGAN"
     gene            4059..4682
                     /locus_tag="VFI55_16625"
     CDS             4059..4682
                     /locus_tag="VFI55_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2466c family mycothiol-dependent reductase"
                     /protein_id="HET7075688.1"
                     /translation="MPEKAPETGQADFWFDPLCPWCWITSRWILEVQKVRDIDVHFHV
                     MSLAVLNENREDLPEQYRENMKRAWGPVRVAIAAEQAHGAGVLAPLYTAMGTRIHNEG
                     NKDLDEVIKQSLADAGLPAELAAAAETDAYDDALRKSHHAGMDAVGDDVGTPTIHVNG
                     VAFFGPVLSKIPRGEEAGQLWDASVTFASYPHFFELKRTRTEPPEFD"
     gene            4753..5232
                     /locus_tag="VFI55_16630"
     CDS             4753..5232
                     /locus_tag="VFI55_16630"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016888270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HET7075689.1"
                     /translation="MRIYLGSDHAGYELKQQIIEHLKKTGHEPIDCGAFSYDPEDDYP
                     AFCIAAATRTVADPDSLGIVLGGSGNGEQIAANKVPGVRCALAWSVETATLARQHNDA
                     QLIGIGGRMHTVEEALTFVDAFVNTPFSKAERHQRRIDILAEYESTHQAPPVPGAGA"
     gene            5254..6069
                     /locus_tag="VFI55_16635"
     CDS             5254..6069
                     /locus_tag="VFI55_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET7075690.1"
                     /translation="MPEGHTLHRLARLHQRRFGGAPVAVSSPQGRFASSAAAVDGRVL
                     RRTSAWGKHLFHHYAADAIVHVHLGLYGAFTEWPVPAGSTGLPLPEPVGQVRMRMVGA
                     DYGTDLRGPTVCEVIDEGRVSDVLAKLGPDPLRSDADPSWAWGRIVKSRRPVGALLMD
                     QTVIAGVGNVYRNELLFRHRIDPFRPGRQISEAEFDAAWTDLVALMKVGLRTGRIVVV
                     CPEHDHGPPSYLPGRPRTYVYRRAGEQCRVCGGPIRTVVLEGRNVFWCPACQS"
     gene            complement(6564..7760)
                     /locus_tag="VFI55_16640"
     CDS             complement(6564..7760)
                     /locus_tag="VFI55_16640"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7075691.1"
                     /translation="MSDLKTKPGIKGSCASDFVAVRDAFERNFVEGKEVGAAVAAWVD
                     GDLVVNLWGGSADAAGTRPWRQNTLTTVLSGTKGLSSTCVHQLADRGELDLQAPVARY
                     WPEFAQAGKEAITLAMVLGHRSGVIAPRAPLSWEEVTDWDFVCDQLAAAEPWWEPGTA
                     QGYHMTTFGFIVGEVFRRVTGRTIGQYLRTEIAEPVGADVHIGLSSADQRRCAERVNK
                     PHMRDLLADLSVPSHPTSLAEHPKAGLAVAMGFVPDDEVSSYDLPLWRQLEFPGTNAQ
                     VSALGLATFYNALAQEKLLSREHMEVVRQCQGGSETDLVLGPRVADHGWGLGYMLNQH
                     CVNGPNPRIFGHGGLGGSFGFVDLEHRIGYAYVANRFDATKANADPRSLALSNEIYAA
                     LGVSSH"
     gene            complement(7792..7862)
                     /locus_tag="VFI55_16645"
     tRNA            complement(7792..7862)
                     /locus_tag="VFI55_16645"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7828..7830),aa:Gly,seq:tcc)
     gene            8008..8084
                     /locus_tag="VFI55_16650"
     tRNA            8008..8084
                     /locus_tag="VFI55_16650"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8042..8044,aa:Pro,seq:tgg)
     gene            8163..9635
                     /gene="tig"
                     /locus_tag="VFI55_16655"
     CDS             8163..9635
                     /gene="tig"
                     /locus_tag="VFI55_16655"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET7075692.1"
                     /translation="MKSSVEQLSPTRVRINVEVPFTELEPEFQRAYKELAKQVRLPGF
                     RPGRAPAKLLEARFGREALLDQVVSEALPDRYGQAVAESEVHPLGQPNIEVTKKEYGQ
                     ELAFTAEVDVRPKISLPDLGALKVSVDPIEVGDDDVDAELESLRARFGTLTGVDRPVA
                     LGDFVSIDLSATVDGEDVANAAAEGLSYEVGSGRLIEGIDDALVGLSVDESREFTAKL
                     ATGEHAGKDAQVTVTVRSIKERELPDPDDEFAQLASEFDTIDELRSNLRDQVRHAKRA
                     QQAERIRNATLDALLEQVEVPLPEAIVQAQFDSAVEGALSSFNHDEIKFAEVLRGQGK
                     SRQQFEVDARNAAETDVQRQLLLDALADDLAVEVSQNDLTERLVVTSRQYGIEPQQLF
                     SYLQENNQLPAMFADVRRGLAIAAAVAAATVTDNDGNVIDTSDFFPARGGQEADSLSQ
                     EADEAEPVGENDEAEPVGEAEEAEPVAKAEAQDASVDETK"
     gene            9762..10352
                     /gene="clpP1"
                     /locus_tag="VFI55_16660"
     CDS             9762..10352
                     /gene="clpP1"
                     /locus_tag="VFI55_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent CLP protease proteolytic subunit
                     ClpP1"
                     /protein_id="HET7075693.1"
                     /translation="MRSNSQGLNLTDSVYERLLSERIIFLGSEVNDEIANRLCAQILL
                     LAAEDATKDINLYINSPGGSISAGMAIYDTMVLAPCDIATYAMGMAASMGEFLLAAGT
                     KGKRYALPHARILMHQPLGGVTGSAADIAIQAEQFAVIKKEMFRLNAEFTGQPIERIE
                     ADSDRDRWFTAPEALEYGFVDHIITRATHITNGEAQ"
     gene            10349..10993
                     /gene="clpP2"
                     /locus_tag="VFI55_16665"
     CDS             10349..10993
                     /gene="clpP2"
                     /locus_tag="VFI55_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent CLP protease proteolytic subunit
                     ClpP2"
                     /protein_id="HET7075694.1"
                     /translation="MSTQHPNIQPQARYILPSFIEHSSFGVKESNPYNKLFEERIIFL
                     GVQVDDASANDIMAQLLVLESLDPDRDITMYINSPGGGFTSLMAIYDTMQYVRADIQT
                     VCLGQAASAAAVLLAAGTPGKRMALPNARVLIHQPSLSGVIQGQFSDLEIQAAEIERM
                     RTLMETTLARHTNKDPAVIRKDTDRDKILTAEDAKEYGIIDTVLEYRKLSAQTA"
     gene            complement(11011..11892)
                     /gene="mmuM"
                     /locus_tag="VFI55_16670"
     CDS             complement(11011..11892)
                     /gene="mmuM"
                     /locus_tag="VFI55_16670"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HET7075695.1"
                     /translation="MALPGESVLIGDGGLATELEARGHDLSDPLWSAKLLAEAPREIV
                     EVHAAYFRAGASIATTASYQASFEGFAARGIGRREAGELLRRSVELAKTARDEARADG
                     LADDLCVAASVGPYGAALADGSEYRGRYGLSSAALMRWHQPRLEILAAAGPDVLALET
                     VPDVDEAEALVNLVRSLGVPAWLSYTIDGTRTRAGQPLAEAFAVAAGVDEIVAIGVNC
                     CTPDDVLPAIRLANIGKPVIVYPNSGERWDGRAWTGPRRFSAQLAPQWVAAGARVIGG
                     CCRVGPGDIAELARAVA"
     gene            12234..13514
                     /gene="clpX"
                     /locus_tag="VFI55_16675"
     CDS             12234..13514
                     /gene="clpX"
                     /locus_tag="VFI55_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET7075696.1"
                     /translation="MARIGDGGDLLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEELADADDVKLDELPKPAEIRDFLEGYVIGQDTAKRTLAVAVYNHYKRIQAGEKS
                     RDSRAEPVELTKSNILMLGPTGCGKTYLAQTLAKMLNVPFAIADATALTEAGYVGEDV
                     ENILLKLIQAADYDVKRAETGIIYIDEVDKIARKSENPSITRDVSGEGVQQALLKILE
                     GTQASVPPQGGRKHPHQEFIQIDTTNVLFIVAGAFAGLEKIIYERVGKRGLGFGAEVR
                     SKAEIDTTDHFADVMPEDLIKFGLIPEFIGRLPVIASVTNLDMDSLVKILSEPKNALV
                     KQYIRLFEMDGVELEFTDDALEAIADQAIHRGTGARGLRAIMEEVLLPVMYDIPSRDD
                     VAKVVVTKETVQDNVLPTIVPRKPSRSERRDKSA"
BASE COUNT         2393 a         4451 c         4644 g         2210 t
ORIGIN      
        1 catggtgatc tgcggcaagg ccgacgtggt gcacgacgac ccggcggggc cggtcatcca
       61 ctccgcgacc aggtcgatcg tggtgccctc ggtgatccag ctgcggtcct acgtgcgggt
      121 tccataccgg gcccgcgtgc cgatgacccg cgccgcgctc atgcaccgcg accgtttctg
      181 ctgtgcgtac tgcggggcga aggcagacac cgtcgatcac gtggtgccac gcagccgcgg
      241 cggtgaccac tcgtgggaga actgcgtcgc gtgctgttcg acctgcaacc atcgcaaggg
      301 cgacaagctg ctcaccgaac tcggttgggc actgcgccgc gtgccgctgc cgccgacggg
      361 acagcactgg cggctgctgt cgacggtcaa ggaactggac ccggcctggg cccgatacct
      421 cggtgaaggc gcggcctgaa ccctgccgtc cgggcgtcgc gcacaatctc gtgtcgcagg
      481 ggtgcgggta gctcacccgg tgggatacgg tttgcgtcgt gagccttgtc gaagtccacc
      541 tgtatgtgct gggaatcccg ctgttgctgg tgattgtgct gtcggtgctg atctggtcga
      601 agaagggacc ccaccccgcg acctacgagc tgtcggagaa gtggacccat ccgccgatcc
      661 tgtgggcagc tgtcgacgag gacgtcggcg gcgcgcacgg cggacacggt acgtcggagt
      721 tctcggttgg aggtggagcc agtggcacgt ggtgaggtag cgacgatcgc ccgcagcgag
      781 ctgcccaagg gctcggtgat caccaccagc gggcggatct cgggagtgac cgagcccggg
      841 gcggtgtcct ccgattaccc attcccgatc acggacctcg tcgccatcga cgacgcactg
      901 aagtacgcct cgcgggaatc gggggcacgg ttcgccatct acctcggcga cctgggcagc
      961 gatacggcgg cacgggctcg cgagatttgg gccaaggtgc ccacgccgga caacgcggtg
     1021 ctgctcgccg tgtcgcccaa ccagtccgcc atcgaggtgg tctacggctc cgcggtcaga
     1081 gggcgtggcg ccgagtccgc cgcaccactc ggggtggccg ccgcttcgtc ggcattcgag
     1141 cagggtcacc tggtcgaggg gctggtcagc gccgtcgtgg agctcagcga caacatctcg
     1201 cgggcttagt cctccgggtc gccctgagcg cgcgagcgtg cgcgaaatgc cgggcaaggc
     1261 ggcgtgtcgt cgtgcggaca cgcacgctcg cgcggcctag ttggcgccgt cgaacgcgcg
     1321 ggcccgcagg gcccgcttca ccccggcctg gccctcgagc accagccgcc gcaaggccgg
     1381 cggcacctcc gactcagggg cggtcaggaa ctcgtcggcg gccgcgacgc cggcgtcgct
     1441 gatgtcccag tgcggataca ggccgaacac cacggtctgg gcgacctcgc tggatcgccg
     1501 cgcccagacc cggggtattg ccgcgaagta gcgcgcgacg aaaggctgga gcagttcacc
     1561 ttgtcccggc gccgcgatgc ccgcgatcat cgcgcgggcc gtgatgttgg gcagggcgtc
     1621 gtcctcgacg accgaggtcc aggcgtcctc cttgacctgt aattgcgggc gcgccgccgc
     1681 ggcctgagcg ccgtgacgtt tgccggctgc ggtcgggtcg cgctgcactt cggcgtcgat
     1741 gaacggtgtc gcgggcccgt cggcgtcgat gttccccgcg gtggccagcg cggtcacgat
     1801 gcgccagcgc aggtcggtgt cgatctcgag gccggccagg cccaggccgg cgggatcact
     1861 gttggccagc gccgccaagg tcttcacgtg ccgcggggac aagaccgacg agcacagcgt
     1921 gttgacgaac gcgagctggt gatccgatcc gggctgcgcg gcacgcgcca gttccaccag
     1981 ccggtcggcg aactgcggcc aaccgtgctc acgcgcccaa gccggctcgg cgtaggaccc
     2041 gagcgccgtc tgggcctgca gcagcagccg ttgcgccacc cccacctcgg tctcggcgtg
     2101 cacgccgccg gagaccagcg ccacgaagtc gcgggcccgc agctcagcct cccgggtcat
     2161 ctcccacgcc gctgaccacg ccagcgtgcg gggcagcggc tcggcgatgt cggcgatccg
     2221 ggcaagcgcc gtctgcagcg aatgcgggtc cagccgaacc gagcagtagg tcaggtcgtc
     2281 atcgttgacc agaatcagct tgccgcgtga gacaccaacc agcgcaggga ctttcgtctc
     2341 cggcccgtcg acgtcgagtt ccacctggtg tacgcggacc agcttgccgg atccgtcatc
     2401 gtcgtagatc ccgaccgcca gccggtgcac ccgggtttca ccggcgccgg gcgtcgcgcc
     2461 gctctgcgtc accgcgaacc gggtgaactt gccgtccgcg tcgacgtcga agtcgggccg
     2521 cagcgtgttc agcccggtgg ttttcagcca ctgctgaccc caagtcgaca agtcacggcc
     2581 ggatgcgttc tccagcgcgg cgagtaggtc gccgaacgtc gcattgccga atgcgtgggc
     2641 gcgaaagtaa tcgcgcagcc ccgccaggaa ttgctccagc cccacgtagg cgacgagctg
     2701 cttgaggacg gaggcgcctt tggcgtaggt gatgccgtcg aaattcacct cgacggcggc
     2761 caagtcgggg atctccgcgg cgatcgggtg cgtcgacggc agctggtcct ggcggtacgc
     2821 ccaggacttc tcgacgttcg cgaacgtcgt ccaggcctcg gtgaattcgg ttgcctcgct
     2881 taggcacaac accgacgcga aggtcgcgaa cgactcgttg agccacaaat cgtcccacca
     2941 ggtcatcgtg accaggtctc cgaaccacat gtgcgccatc tcgtgcagca ccgtctcggc
     3001 gcgtcgctcg taggacgctc gggtcaccct gctgcggaac acgtagtcct cgagaaaggt
     3061 gaccgcgccg gcgttctcca tcgcgccggc gttgaattcg gggacgaaca gctgatcgta
     3121 cttgccgaac gcgtagggaa ggccaaagtt gttgtggtag aagccaaatc cctgcttggt
     3181 ctgggtgaac agccgctcgg cgtccatgtg tccggccagc gaggcgcggc agtagatgcc
     3241 tagcgggatc tcgccgtgtt cgtcgacgta ggagtctttc cactcggcat acggtccggc
     3301 gatcaacgcc accagataag tgctcatccg cggcgtggtg gcaaaggtgt gcacactctt
     3361 ttcggcggag ttctctccgt ccccgacgga ctcgatggac tcggtggcgc cgttggagat
     3421 caccttccag tgcttgggag cgagcacccg caggttgaac gtcgccttga ggtcgggctg
     3481 gtcgaagcag gcgaacatgc gtttggcatc cgcggtttcg aactgcgagt acaggtaggt
     3541 ctcgccatcg acggggtcga cgaagcggtg cagtccctcg ccggtgttgg agtagctgca
     3601 gtcagtgtcg atgacgacga cgttgcgatc ggccagtccg cgcagcggga tgccggtcga
     3661 ctcgtcgtat ccggagacgt ccagttcgcg tccgttcagg gtggcgctgc gaacggtgtc
     3721 ggcggcgatg tcgatgaccg tgtcggcccc ggcgagtgcc tcgaagacca cggtggtggt
     3781 ggaccggaaa gttcgttcgc cgggcgcgct tcgatcgtct gagccgtccg ggccgtcggt
     3841 gacgtcgagg ttgatctggt agctgtcgac ggtgatcaac gcggcgcgtt cgacagcctg
     3901 gtcccgagtg aggtttggaa gggccacgcg tccaacttaa acgtgtcggc gaacactggg
     3961 taaccgaaga ggtggggcgg gaacacgtgc cgcgtgacta cggttgtgct gaggggcgtg
     4021 attaccgtct ctaaatattc ccggagagga atgctgcgat gcccgaaaag gcccccgaaa
     4081 caggccaggc tgacttctgg ttcgacccgc tgtgtccatg gtgctggatt acgtcgcgct
     4141 ggatcctcga ggtgcagaaa gtccgcgata tcgacgtgca cttccacgtg atgagcctcg
     4201 cggtgctcaa cgaaaaccgc gaagacctgc ccgagcagta ccgcgagaac atgaagcggg
     4261 catggggacc ggtgcgggtg gcgatcgccg ccgagcaggc ccacggtgcc ggtgtgctgg
     4321 cgccgctgta tacggcgatg ggcacccgga ttcacaacga gggcaacaaa gacctcgacg
     4381 aggtcatcaa acagtcgctc gctgacgccg gtctgccggc cgagttggcc gcggcggccg
     4441 aaaccgacgc gtacgacgac gcgctgcgca agagtcacca cgcggggatg gacgcggtcg
     4501 gcgacgacgt cggcacgccg accatccacg tcaacggtgt ggcgttcttt gggccggtgc
     4561 tctcgaagat cccgcgcggc gaggaagcgg gccaactctg ggacgcctcg gtgaccttcg
     4621 cgtcctaccc gcactttttc gagctcaagc ggacccgcac cgagccaccc gaattcgact
     4681 gaccgccaaa cactcagcga cgtaaggcct cgcgcgttgc gtcgccgagt tcggcgggta
     4741 aggatgtcgg gcatgcgcat ctacctgggt tctgaccatg ccggctatga gctcaagcag
     4801 cagatcatcg agcacctcaa gaaaacaggg cacgagccga tcgattgcgg tgccttcagc
     4861 tacgaccccg aagacgacta cccggcgttt tgcatcgccg ccgcgacccg cacggtggcc
     4921 gaccccgaca gcctgggcat cgtgctgggg ggatcgggca atggtgaaca gatcgccgcc
     4981 aacaaggtcc cgggggtccg ctgcgcgctg gcgtggagtg tggagaccgc gacactggcc
     5041 cgtcagcaca acgatgccca gctgatcggc atcggtgggc gcatgcacac cgtcgaggag
     5101 gcgctgacgt tcgtcgacgc cttcgtcaac acgccattct cgaaagccga acgccatcaa
     5161 cggcgcatcg acattctcgc cgagtacgaa agcacccatc aggcgccgcc ggtgcccggc
     5221 gcgggggcct gagcccagaa ctgagagtcg caagtgcccg aagggcatac cctgcaccgg
     5281 ctggcccggc tgcaccagcg ccgcttcggc ggggcaccgg tcgcggtgtc cagcccgcag
     5341 ggccggttcg ccagttcggc ggccgcggtg gacggccggg tgctgcggcg gaccagcgcc
     5401 tggggtaagc acctgtttca tcactacgcc gccgacgcga tcgtgcacgt gcatctcggt
     5461 ctgtacggcg ccttcaccga atggccggtt cccgcaggct cgaccgggct accgctgccc
     5521 gaaccggtcg gacaggttcg gatgcggatg gtcggtgccg actacggcac cgacttgcgc
     5581 ggtccgacgg tgtgcgaagt catcgacgaa ggccgggtca gcgacgtgct ggccaagctc
     5641 ggtcccgacc ccttgcgcag cgacgccgac ccgtcgtggg cgtggggccg aatcgtcaag
     5701 tcccgcaggc ctgttggcgc gctgttgatg gaccagacgg tgatcgccgg ggtgggcaac
     5761 gtttaccgca acgagttgct gttccgccac cgcatcgacc ctttccggcc cggccgacag
     5821 atcagcgagg cggagttcga tgcggcgtgg acggacctgg tggcgttgat gaaggttggt
     5881 ctgcgcaccg gcaggatcgt ggtggtgtgc cccgaacatg accacggtcc gccgtcgtac
     5941 ctgcccgggc ggccccggac gtatgtgtac cggcgcgcgg gcgagcagtg ccgggtgtgc
     6001 ggggggccga tccgcacggt ggtgctggag ggacgcaacg tgttttggtg cccggcctgc
     6061 caaagctgag cgtgtgtacc cctcggggcg aaactcgccg gtcattggtc gatcgcccac
     6121 aggaaactct cagcaagaaa cgctggcgtc tatagtttga cttccacatc gcgtgggcaa
     6181 ccgttgttct cgtgaaaact attcgacgtt tggttgcaac gtttttcgcc atagccgtcg
     6241 gactggggct ggcggggctt ggcgccgcca cggaggccca agcgcagccg gggcccttcc
     6301 cgcaatggtg cccgggggag ttctgggacc cgggctgggg cgataactgg gattggggcc
     6361 gctgccacga ctggcggggg ggacccggag gctgggaccg tgaccacgcg gggcctggat
     6421 gggatcggga ccacccggga cctggcggac cttggcagcc gaacccctgg caacggtaat
     6481 tccagggtaa tttcagcgct gttcgatgaa cccgggggcc gcgagccgcc tgtggtgcgg
     6541 ttcgcggccc ataattttcc aggtcagtgc gagctgactc cgagcgcggc gtagatctcg
     6601 ttactgagcg cgaggctgcg agggtcggcg ttggctttgg tggcgtcgaa acggttcgcc
     6661 acgtaggcgt agccgatccg gtgttccagg tcgacgaagc cgaatgagcc gccgaggccg
     6721 ccgtgcccga agatccgcgg gttggggccg ttcacgcagt gctggttgag catgtagccc
     6781 agaccccagc cgtggtcggc cacccgcggc ccgagcacca gatcggtctc cgagccgccc
     6841 tggcactgcc ggaccacctc catgtgttcg cggctgagca gcttctcctg agcgagcgcg
     6901 ttgtagaacg tggccaatcc cagcgcggac acctgcgcgt tggttccggg gaattcgagc
     6961 tggcgccaca gcgggaggtc gtaggagctg acctcgtcgt cgggcacgaa tcccatcgcc
     7021 acggccaggc ctgccttggg gtgttcggcc agggaggtgg gatggctggg gacggacagg
     7081 tcggcgagca aatcgcgcat gtgcggcttg ttgacccgct cggcgcagcg acgctggtcg
     7141 gccgatgaca agccgatgtg gacgtcggcg ccaacgggct cggcgatctc ggtgcgcagg
     7201 tattgaccga tcgtgcggcc cgtcacacgc cggaacacct cgccgacgat gaatccgaag
     7261 gtcgtcatgt ggtagccctg ggcggtgccc ggctcccacc acggctccgc ggccgctaac
     7321 tggtcgcaga cgaagtccca gtcggtgacc tcctcccagc tcagtggggc gcgcggcgcg
     7381 atgacaccgg atcggtgccc gagcaccatg gccagcgtga tcgcttcctt gccggcctgg
     7441 gcgaactcgg gccaatagcg ggccaccggc gcctgcaggt ccagctcgcc gcggtcggcc
     7501 agttgatgga cgcaggtgga cgaaagaccc ttggtgcccg acagcaccgt ggtcagggtg
     7561 ttctgtcgcc agggccgggt tccggcagcg tcggccgagc cgccccacag gttgacgacg
     7621 aggtccccgt caacccacgc cgcgaccgcg gcgccgacct ctttaccctc cacgaagttg
     7681 cgctcgaacg cgtcgcgcac ggcgacgaag tccgacgcgc atgagccctt gatgccaggt
     7741 ttcgttttca ggtcgctcat ggcgcctttc tttcgagcag aacttagccc gagcgggcga
     7801 cgggaatcga acccgcgtag ctagtttgga agactagggc tctaccattg agctacgccc
     7861 gcaagtttta gtcgagcgcg actgtatctg gcggcgcaca tcaaatctaa tcgacgcggg
     7921 tttgtgaccg ctctgtgaga tctcgagctt cctgcgaagc cggtggccaa cctggtgatg
     7981 aagggccgta ggatcgcgag gtcagcgcgg ggtgtagcgc agcttggtag cgcatccgct
     8041 ttgggagcgg aaggccgcag gttcaaatcc tgtcaccccg accagcgcga ccagcctgta
     8101 ccgcacctgg gcggcggtta gggcccaccg acagccagga acgatcaccg aggagcacat
     8161 ccgtgaagag cagcgtcgag cagttgagcc ccacccgggt gcgcatcaac gttgaggtgc
     8221 ctttcaccga actggagccc gaattccagc gggcctacaa agagctggca aagcaggtgc
     8281 ggctgcctgg cttccggccc ggcagggcac cggcgaagtt gctggaagcc cggttcggca
     8341 gggaagcgtt gctcgatcag gtcgtcagcg aggcgctgcc cgaccgctac ggacaggccg
     8401 tcgccgagtc ggaagttcat ccgctcggcc agcccaacat cgaggtcacc aagaaggagt
     8461 acggccagga gctggcgttc accgccgagg ttgacgtccg ccccaaaatc agcctgcccg
     8521 acctcggcgc gctgaaggtc tcggtggatc cgatcgaggt cggcgacgac gatgtcgacg
     8581 ccgagctgga gtctctgcgg gcccggttcg gcaccctgac cggggtggac cggccggtgg
     8641 ccctcggcga cttcgtgtcg atcgacttgt cggccaccgt cgatggcgag gatgtggcga
     8701 acgcggccgc ggagggactg tcctacgagg tcggttcggg caggttgatc gaaggcattg
     8761 acgatgcgct ggtcggtttg tccgtcgacg agtcccggga attcaccgcg aaattggcga
     8821 cgggcgaaca cgccggcaaa gacgcgcagg tcaccgtcac tgtcaggtcg atcaaggaac
     8881 gcgagctacc ggaccccgac gacgagttcg ctcagctggc cagcgagttc gacaccatcg
     8941 acgagctgcg gtccaacctg cgcgaccagg tgcgtcacgc caagcgcgcc cagcaggccg
     9001 agcggattcg caacgccacg ctggacgctc tccttgagca ggtcgaggtg ccgctgccgg
     9061 aggcgatcgt gcaggctcag ttcgacagcg ccgtcgaggg cgcgctgagc agcttcaacc
     9121 acgacgagat caagttcgcg gaggtgctgc gggggcaggg caagtcacgc cagcagttcg
     9181 aggtcgacgc tcgcaacgcc gccgagacgg acgttcagcg gcagctgttg ctggatgcgc
     9241 tggccgacga tttggcggtc gaggtgagtc agaacgacct caccgaacgg ctggtggtga
     9301 cgtcccggca atacggcatc gagccgcagc agttgttctc ctacctgcag gagaacaacc
     9361 agctcccggc catgttcgcc gacgtgcggc gcggcctcgc catcgccgcg gccgttgcgg
     9421 cggcgaccgt taccgacaac gacggcaacg tcatcgacac cagcgacttc ttccccgcgc
     9481 gcggcggcca ggaggccgac agtttgagcc aggaggccga cgaggccgag ccggtgggtg
     9541 agaacgacga ggccgagccg gtgggtgagg cggaggaggc cgaaccggtc gctaaggccg
     9601 aagcgcagga cgcctcggtc gacgaaacga aatgaccccc tgtgacgctg tgagcgaacg
     9661 cgcccatttc agggggtgcg ggaccggcgg acagcgggct tggttggtta gtgtcggtgc
     9721 atacggaatt cgagaaagca ggtaacccag tcgtgactga catgcgttcg aactcgcagg
     9781 gtctcaacct cacggactcg gtctatgagc gcttgctctc cgagcgcatc atcttcctgg
     9841 gctcagaggt caacgacgag atcgccaacc ggttgtgcgc gcagatcttg ttgctcgccg
     9901 ccgaagacgc caccaaggac atcaacctct acatcaattc acccggtgga tcgatcagtg
     9961 ccgggatggc catctacgac accatggtct tggcgccgtg cgacatcgcc acttatgcga
    10021 tgggaatggc cgcctcgatg ggcgaattcc tgttggcggc gggcaccaag ggcaagcgct
    10081 acgcgttgcc gcacgcccgc atcctgatgc accagccgtt gggtggcgtg accggtagcg
    10141 cggccgatat cgcgatccag gccgagcagt tcgccgtcat caaaaaggag atgttccggc
    10201 tcaacgccga attcaccggg cagccgatcg aacgtatcga ggcggattct gatcgcgacc
    10261 gctggttcac cgccccggaa gccctggaat acgggttcgt cgaccacatc attacccgcg
    10321 ccacccacat cactaacgga gaagcccagt gagtacccag caccccaaca tccagcccca
    10381 ggcgcgctac atccttccgt cgttcatcga gcactcgagc ttcggcgtca aggaatcaaa
    10441 cccctacaac aagctgttcg aagaacgcat catcttcctc ggcgtgcagg tcgacgacgc
    10501 gtcggcgaac gacatcatgg cgcagctgct ggtcctggag tcgctcgacc ccgaccgcga
    10561 catcaccatg tacatcaact caccgggcgg cgggttcacc tccctgatgg cgatttacga
    10621 caccatgcag tacgtgcgcg ccgacatcca gacggtgtgc ctgggccagg ccgcatcggc
    10681 ggcggcggtg ctgctggccg ccggcacacc cggtaagcgg atggcgctgc ccaacgcgcg
    10741 ggtgctgatt caccagccgt cgctgtccgg cgtgatccag ggccaattct ccgatctgga
    10801 gatccaggcc gcagagatcg agcggatgcg caccctgatg gagaccacgc tggcccgcca
    10861 caccaataag gatcccgcgg tcattcgcaa agacaccgac cgggacaaga tcttgaccgc
    10921 cgaagatgcc aaggaatacg ggatcatcga caccgtgctg gagtaccgca agctttccgc
    10981 gcagaccgcc tgaggtccgg cgagcggacc ttacgccacg gccctcgcca gttcggcgat
    11041 gtcgcccggc cccacccggc agcatccgcc gatgactcga gcgccggccg ccacccactg
    11101 gggcgcaagc tgcgcactaa acctgcgcgg tcctgtccac gcccgcccgt cccatcgctc
    11161 accgctgttg gggtagacga tgacgggctt gccgatgttc gccaagcgga tcgcgggcag
    11221 cacgtcgtcg ggcgtgcagc agttgacgcc gatcgcgacg atctcgtcga ccccggcggc
    11281 caccgcgaac gcttcggcga gcggttgtcc ggcgcgggtc cgggtcccgt cgatggtgta
    11341 gctgagccag gccggcaccc ccagcgaacg caccaggttg accagcgctt cggcctcgtc
    11401 gacgtcgggc accgtttcga gggcgagcac gtccgggccg gcggcggcca ggatctccag
    11461 ccggggctga tgccaccgca tcaacgccgc gctggacaag ccgtagcgac cgcgatattc
    11521 ggacccgtcg gccagcgccg cgccgtacgg gccgaccgag gcggcaacgc aaaggtcatc
    11581 tgcgaggcca tctgcgcggg cctcgtcgcg cgcggtcttg gcgagttcga cgctgcggcg
    11641 cagcagctcg ccggcctcgc gacgtccgat gccgcgggcc gcaaagccct cgaacgatgc
    11701 ctggtagctc gccgtcgtcg cgatcgacgc accggcgcga aagtacgcgg cgtgcacctc
    11761 gacaatctcc cgcggagcct ccgccaacag cttggccgac cacaacgggt cggacaggtc
    11821 gtgaccgcgc gcttcgagct cggtggccag gcctccatcg ccgatcagca cggattctcc
    11881 aggcagagcc aaccccactc agccactgta aggttgttgc ttgaaggtcc gcccaaacct
    11941 cgcttgaccg ccgggttggg gtcggcacct tcagtggcac cagggtctgg cggcgcctcc
    12001 agcctcggtc gggtaacgac cgcgacacgc caaagacacg gattgcgcgt ctcggcgagt
    12061 gacgggcacc gtctgcctat atgtttcatt cagacggaca ggcatcaggc catgaatacc
    12121 aaggacgcga ttcggcgcta atggtcgcgc cgggcccgag caagcgggta gcgtcgtgga
    12181 ttacatgcgg catagcaaga cgaacggcga acaggaagta ggccctcagc accatggcgc
    12241 gcattggaga cggtggcgac ctgcttaagt gctcgttctg cgggaagagc caaaagcaag
    12301 tcaagaaact cattgccggc cccggtgtgt acatctgtga tgaatgcatc gatctctgta
    12361 acgagatcat cgaagaggag ctcgccgacg ccgacgacgt caagctcgat gaactgccca
    12421 agcccgctga gatccgggat ttcctcgagg gatacgtcat cgggcaggac accgccaagc
    12481 ggacgctggc cgtcgcggtc tacaaccact acaagcggat ccaggccggt gagaagagcc
    12541 gtgactcccg ggccgagccg gtggagctga ccaagtccaa catcctgatg ctcggaccga
    12601 ccggctgcgg gaagacctac ctggctcaga cgctggccaa aatgctcaac gtgccgttcg
    12661 ccatcgccga cgccaccgcg ctgaccgagg ccggttacgt cggcgaggac gtcgagaaca
    12721 tcttgctgaa actcatccag gccgcagact acgacgtcaa gcgtgccgag accgggatca
    12781 tctacatcga cgaggtcgac aagatcgccc gcaagagcga gaacccgtcg atcacccgcg
    12841 acgtctccgg cgagggcgtg cagcaggcac tgctgaaaat cctggagggc acccaggcgt
    12901 cggttccccc gcagggcggc cgcaagcacc cgcaccaaga gttcatccag atcgacacca
    12961 ccaacgtgct gttcattgtc gcgggtgcgt tcgccgggct ggaaaagatc atctacgagc
    13021 gcgtcggcaa gcgtgggctg ggcttcggcg ccgaggtccg ctccaaggcc gagatcgaca
    13081 ccaccgacca cttcgccgac gtgatgcccg aggatctcat caagttcggc ctgatccccg
    13141 aattcatcgg ccggctgccg gtcatcgcgt ctgtgaccaa cctggacatg gactcgttgg
    13201 tcaagatcct gtccgagccg aagaacgctt tggtcaagca gtacatccgg ctcttcgaga
    13261 tggacggcgt ggaactggag ttcaccgacg acgcgctgga ggcgatcgct gaccaagcga
    13321 tccaccgcgg caccggcgcc cgtggcctgc gggcgatcat ggaagaagtc ctgctgccgg
    13381 tcatgtatga catcccgagc cgcgacgacg tcgccaaagt tgtggtgacg aaggagaccg
    13441 tgcaggacaa cgtgctgccg accatcgtgc cgcgcaagcc gtcgcgcagt gagcgccgcg
    13501 acaagagcgc ctagcccggc gacgatggcg gcagggattg ccgcgtgagg agtcgggcaa
    13561 tccagcccta gcccggcgac gatggcggca gggactgccg tgtgaggagt cgggcaatcc
    13621 agccctagcc aggcgacgat ggcggcaggg attgccgcgt gaggagtcgg gcaatccagc
    13681 cctagcccgg cgacgatg
//
LOCUS       DASSGQ010000379        18533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_451816,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000379 DASSGQ010000000
VERSION     DASSGQ010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18533
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451816"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VFI55_16680"
     CDS             complement(<1..835)
                     /locus_tag="VFI55_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075697.1"
                     /translation="MQLTDLARWTPVRFDFSGPAPALYWADLSAERFTEPFFDQTVDR
                     WASGPHARPLVKTGLDALVALDGEPSLDPAGMIFHLSRCGSTLVSRLLGTLPGVVVVA
                     EPAPLNTLLALDPDRVDEAALARAVRLMVRALGRRRHGDERRLVLKCTSWNVRRRAVL
                     AAAFPETPWIWVQRDPTLVLASLLAKPPGWLGLQAAPQRAARRFGLDPAAVPTMSRAE
                     FAARTLGAMLEAAADRAGRLCIDHADLPNAVWQRVAPHFGLEADPAAIQRMIEESRLY
                     SK"
     gene            complement(855..1994)
                     /locus_tag="VFI55_16685"
     CDS             complement(855..1994)
                     /locus_tag="VFI55_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7075698.1"
                     /translation="MEAFLDKIWAATHLHVDRHSAGYFDSLLPGPSALDELLELYRVE
                     PSAVRLMREKDKRGSDSYRLVDGNLDLVGIRNDFADGFTIVVDGVERYVRAIASLAHS
                     VEVELNFPTQVNAYITPPGSRGLVPHYDDHDVLILQIQGSKIWHLYDGTEIPPHEMQR
                     RDKAVPADGLPSPTDLRLQVGDLLYLPRGLVHAADTDAEPSIHLTVGIHAPTALTLAI
                     GALYSLSFHDDRLNAQLPPRHLDDPAVHAKLGALLRDAVRTVEDPGAIAQGLDALADV
                     LVRHGRCPPVGKISNAAGIDGQTLVRKYQPLYSRVKAVANGVALQFATLSIDAGPNHK
                     AAMLFISNSTEPFRVRDLPELGAKQQIELARSLIVGGFLVRLSDG"
     gene            2376..4292
                     /locus_tag="VFI55_16690"
     CDS             2376..4292
                     /locus_tag="VFI55_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPE domain-containing protein"
                     /protein_id="HET7075699.1"
                     /translation="MNFQVLPPEINSLLMFTGAGSAPMLAAAAAWDGLASELGSAASS
                     FGSVTSVLAAQSWQGPASTAMLAAASPYAGWLNVAATQAQRAASQAKAVVGVFESAQA
                     ATVHPLVVAANRDEFVQLVQSNFLGQNAPAIAAAEARYEEMWAADVAAMAGYHGGASA
                     AAAQLSSWQGALPALPGMSQVASAVAGSPVGAAAAAVSMEASTVPGLPALNTGLGNIG
                     SWNLGGGNIGNTNLGSGNVGNTNLGSGNIGSANLGSGNIGNTNLGSGNQGSNNFGLGN
                     IGNTNLGSGNVGSNNFGLGNIGNLNLGGGNQGSNNVGGGNLGSNNFGSGNTGNSNVGF
                     GNLGSNDFGFGNSGSGDIGIGLTGNNQEGINFAGLLNSGAGNFGFGNAGNNNIGFFNS
                     GDGNFGIFDSGNGNFGFANSGNTNTGFWNGGSFNSGFGNAADLNFGFANAGPGNMGFG
                     NAGSGNVGVGNAGLFNYGNFNSGGVLGGAGNTGGFNSGNVDTGWFNSGNTNTGLLNSG
                     NLDTGVGSSADLTGLVTNSGFGNSGTGNSGFYNSGDFSSGLLNNGSQSAGLFNTGDVQ
                     TGIFNTGGSNTGFFNRDFDNVGFGNTGQLNSGFSNSGTGDSGFENSGNYDSGALNRGD
                     YQAGILGNGTPDSG"
     gene            4422..5327
                     /locus_tag="VFI55_16695"
     CDS             4422..5327
                     /locus_tag="VFI55_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7075700.1"
                     /translation="MRSEGDTWDITTSVGSTALFVATARALEIQKPDPLAVDPYAEIF
                     CRAVGGPPADVLDGKDPDHPLKTADFGEHFVNFQGARTKYFDEYFGRAVDAGVRQVVI
                     VAAGLDSRAYRLDWPDGTTIFELDRPEVLDFKREVLTSHGAQPRAERREIAVDLREDW
                     PQALRDRGFDTAAPSAWIVEGLLIYLPATAQEQLFTGIDSLAGPGSHVAIEDGAPMAA
                     EEFSAAVEEERAAAAEGDGRVFFQLVYNERHAPATEWFGKRGWTAVGTPLVDYLRETG
                     RPVPGPDTEAGPMIARITLVSAVKG"
     gene            5566..7080
                     /locus_tag="VFI55_16700"
     CDS             5566..7080
                     /locus_tag="VFI55_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPE family protein"
                     /protein_id="HET7075701.1"
                     /translation="MTAPVWMASPPEVHSALLSSGPGPGSLLTAAGSWSSLSATYASV
                     ADELSAILAAVQAGAWEGPSAEQYVAAHVPYLMWLMQASADSAATAAQHETAATAYTV
                     ALATMPTLVELAANHAIHAVLVATNFFGINAIPIALNEAEYAWMWVQAAVAMVQYELT
                     SFSAVTSAPQTETPPQIVKPIQSSDSSDTGGSVQDIVDDDSGDPYQLSWWVNRFFEVS
                     ETLERDILEFPQNPSQAIAQLESDVPGLVADEVGHAAEAYQAFFPEINALALTVSAFN
                     PGFLGGLGGLGALDSIQPDAVAAAPAPTPETPSVPVGSSSSPVLSTAPGPAPSSAPAS
                     APTTAPAQAPATGAPPPPPAATAGAAYPYLVGGPTMGAGAGISSSAQRKALEPETAAA
                     AAAAAASALAKQRARRRRRAGMRGYGDEFMDMNVEVEPDWGNPSAAGPVASTVASDRG
                     AGKLGFAGTTRKETIAEAGGLATLAGDEFGGGPSVPMVPRTWDHDRVPGEGDRS"
     gene            7323..9182
                     /gene="eccA"
                     /locus_tag="VFI55_16705"
     CDS             7323..9182
                     /gene="eccA"
                     /locus_tag="VFI55_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion AAA-ATPase EccA"
                     /protein_id="HET7075702.1"
                     /translation="MERGDTGVLAAQQVNSRSDGRVDGDTVSRFATCCRALGIAVYER
                     QRQADLAAARSGFTALTRIAHEQCDAWTGLAAAGDVSLRVLEATSRTATTAGKLQREV
                     ELAPGALGFRYDTGLYLQFRATTPDDFHLAYAAALASPEAGRFADADRIVSGLIERRP
                     SWREARWVAVVINYRAERWSDVVKLLTPIVNDTDLDETFSHAAKITLGTALARLGMFA
                     PALSYLEEPEGPVVVAAVDGALVKALVLRAHVDEESANEVLQDLYAAHPENEQVEQAL
                     SNTSFGIVTTTAARIDARTDPWDPATEPSVDDFVDPAAHERKAVLLHEAERQLAEFIG
                     LDEVKNQVSRLKSSVAMELVRKQRGLAVAQRAHHLVFAGPPGTGKTTIARVVAKIYCG
                     LGLLKRENIREVHRADLIGQHIGETEAKTNAIIDSALDGVLFLDEAYALVATGAKNDF
                     GLVAIDTLLARMENDRDRLVVIIAGYRADLDKFLDTNEGLRSRFTRNIDFPSYVPAEL
                     VEIAHKMAEQRDSVFEPAALGHMEALFSKLAAESTPDTNGISRRSLDIAGNGRFVRNI
                     VERSEEEREFRLDHSEHAGTGEFTDEELMTITDQDVEKSVEPLLRGLGLSVPS"
     gene            9179..10816
                     /gene="eccB"
                     /locus_tag="VFI55_16710"
     CDS             9179..10816
                     /gene="eccB"
                     /locus_tag="VFI55_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccB"
                     /protein_id="HET7075703.1"
                     /translation="MTTNEPDPERGGARRSFSSRTPVNDNPDQVIYRRGFVTRHQVTG
                     WRFVMRRIASGIALHDTRMLVDPLRTQSRAVLMGALILITGLAGCFVFSLIRPSGQAG
                     NNAVLADRSTDALYVRVGDQLHPVLNLTSARLIVGRPVDPTAVKSSELDRFPRGNLIG
                     IPGAPERMVQNTSHDANWTVCDAVSGSGQGGHGAHSTGVTVIAGPPDSQGARAAKLGS
                     GQAVLVDLSGDMGTSTWLLWDGRRSQVDLANHAVTSALGLGAGVPAPRPIAFGLFNAI
                     PEGPPLAAPTIPNAGAPANFSVPAPIGAVVASYGLDQNSSGGVRYYAVLPDGLQPISP
                     VLAAILRNTNSYGLDQPPRLGADQIAKLPVSRMLDTARYPDQQVALVDATKDPVTCAS
                     WSRPAGAASSSMSLLSGSALPVPDAVHTVDLVGAGYQGVASRVALAPGTGYFAQTVDG
                     DAASPGAGSLFWVSDTGVRYGIDTETGEAANPAGGHGKPVEALGLSAPPVPVPWSILS
                     LFATGPALSRADALLAHDGLAPDSRPGRPVAAEGAPR"
     gene            10813..14808
                     /gene="eccCa"
                     /locus_tag="VFI55_16715"
     CDS             10813..14808
                     /gene="eccCa"
                     /locus_tag="VFI55_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccCa"
                     /protein_id="HET7075704.1"
                     /translation="MSRLIFEARRRLAPPAVRKGTITIEAPPELPRVIPPSLLRRAMP
                     YLIVILIIGMVVALVATGMRVISPQTLFFPFVLLLAATALYRGNDNKMRTEEVDAERA
                     DYLRYLSVVRDNIRTQAAEQRAAAQWSHPEPTELATVPGSRRQWERDPHDPDFLVLRA
                     GRHQVPLATALRVNDAADEIDLEPVSHSALRSLLDTQRTVPDVPTGIDLTKVSRITVL
                     GEPDEVRGALRAWIAQAVTWHDPTVLGVALATRNLEGPDWSWLKWLPHIDIPGELDGV
                     GPARFLAANADDLVALLEPALVERPAFTGGRSDALRHLLIVVDDPDYDLNASALAVGR
                     AGVTVVHRSAKPPHREQYSDPEKPILRIAHGAIERWQTGGWQPYIDAADDLGPDDASH
                     LARQLSRWDSNPTHAGLRSAATRGASFTTLLGIPDASQLDVPTLWAPRRRDDELRVPI
                     GVTATGEPLIFDLKDEAEGGMGPHGLMIGMTGSGKSQTLMSILLSLLTTHSADRLIVI
                     YADFKGEAGADSFRNFPQVVAVISNMAEKKSLADRFADTLRGEVARRETLLREAGRQV
                     QGSAFNSVTEYENARESGAAGAVDLPPIPTLFVVADEFTLMLADHPEYAELFDYVARK
                     GRSFRIHILFASQTLDVGKIKDIDKNTSYRIGLKVASPSVSRQIIGVEDAYHIESGKE
                     HKGLGFLVPAPGATPIKFRSTYVDGIYDPPQAAKSIVVQSILEPKLFSAGRVEPDQDT
                     LISSAGEEETAGPPRKLIATIGEQLERYGPRAPQLWLPPLDEPIPLTAVLARAGIPRQ
                     EWRWPLGEIDKPFEMRRDPLVFDATSSAGNVVIHGGAKSGKSTALQTFILSAASLHSP
                     RDVTFYCLDYGGGQLRAVEDLAHVGSVASGLEPERIRRTFGELEQLLLSRLRREVFKD
                     RNGSAPDDGFGEVFLVIDNLYAFGRDNTDQFNTRNPLLAKVTELVNVGLAYGIHVIVT
                     TPSWLEVPLAMRDGLGLRLELKLHDPRDSNVRVIGALRRPADAVPHDQPGRGLTMAAE
                     HFLFAAPELDQVGAINARYPGTAAPPVRLLPVNLAAGAVRPLYRGPEQVVIGQREEDL
                     APVVLDFTENPLLMAFGDSKSGKTTLLRHIVRTIREHSTEDQIAFTVLDRRLHLVDEP
                     LFPDNEYTANIDRILPAMLGLSNLIESRRPPAGLSAAELSRWTYQGHTHYLIIDDVDQ
                     IPDSPVTSGPYIGQRPWTPLIGQLSQAADLGLRVIVTARASGSGHTLMTSPLLRRFND
                     LQATTLMLSGNPQDSGKIRGQRFNRLPAGRAILLGDSDSPTYVQLVNPLIGESVLASE
                     AQAEGQQKEWP"
     gene            14805..15113
                     /locus_tag="VFI55_16720"
     CDS             14805..15113
                     /locus_tag="VFI55_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HET7075705.1"
                     /translation="MTLRVLPEGLAATSAAVEALTARLAAAHAAAAPAITAVVPPAAD
                     PVSLQTAAGFSAQGQEHAAIAAQGVEELGRAGVGVGQAGASYLAGDAAAAATYGMAGG
                     "
     gene            15116..16687
                     /locus_tag="VFI55_16725"
     CDS             15116..16687
                     /locus_tag="VFI55_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPE family protein"
                     /protein_id="HET7075706.1"
                     /translation="MTGPLVPFGSIVWMASPPEVHSALLSTGPGPGSLLAAAGAWSSL
                     STEYASAAAELSGLLGAVQAGAWQGPTAERYAAAHLPYLEWLQQASADSAGVAAQHEV
                     AATAYTAAVAAMPTLAELAANHVVHGVLVATNFFGLNTIPIALNEADYVRMWVQAATT
                     MAGYQAVAGAALASAPRSIPAPNVVNPGVGETGNAAAATTQGGAATQAAQSGSSLNLS
                     DLLSQLLSEYTNFYGGMYQELGSFLQNPIGNSVQLIEAFLTNPSEALVLYGPLLFAIA
                     WQAFSWVGASITYPSLFLSPFLGLVRPLTLPLALANAVEVAPTLTEPVAGGVAVTAQP
                     VSVQPVVALAPTAAPAAGIPASTPAGAGAAATPAPGAATGSFAYVVGGGGNPETGFGP
                     TLTDRGGTKAPAATIPAAAAAVPSAAQSRARRRRRAAMREYGDEFLDMDSDIGVAPDY
                     GQPEQLVSAMASGNGAGTLGFAGTAHREAALPAAGLTKLAGDEYGGGPRMPMLPGTWE
                     QDNHGAEPGRGGSSN"
     gene            16742..17035
                     /locus_tag="VFI55_16730"
     CDS             16742..17035
                     /locus_tag="VFI55_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HET7075707.1"
                     /translation="MSLLDAHIPQLMAAQSAFSAKAALMRSTISQAEQEALSAQSFHV
                     GEASAAFQAAHARFVEAASRINALLDIAQANLGDAAGTYVAADAAAASGYTAF"
     gene            17068..17358
                     /locus_tag="VFI55_16735"
     CDS             17068..17358
                     /locus_tag="VFI55_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HET7075708.1"
                     /translation="MSEIMYNYPAMLGHAGDMSGYAGTLQGLGADIASEQAALSNAWQ
                     GDTGMTYQVWQAQWNQAMESLVRAYQSMASTHEANTMAMYARDAAEAAKWGG"
     gene            17379..18278
                     /locus_tag="VFI55_16740"
     CDS             17379..18278
                     /locus_tag="VFI55_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESX secretion-associated protein EspG"
                     /protein_id="HET7075709.1"
                     /translation="MNAEPNAVELTVDQAWFIAETIGAGSFPWVLAITAPYCDAAHRE
                     AFCERQRSELIRMGLLSQTGLLNPAVADWIRVVCFPDRWLDLRYVGHAPATGSGPATD
                     DLLRGVVAQRVGAADRAAKTRKTVVALRSAQLITFTAMDIDDARALVPVLGVGLAQRP
                     PARFDAFSMPTRVGARADERLRSGVALAEVVDYLGIPASARPVVESVFCGPRSYVEIV
                     AGCRRDGRHSTTEVGLSVVDSAAGRVLVSPSRAFDGEWVSTFSPGTPFAVAVAIEQLT
                     AELPDGQWFPGQRLARDFSAQHA"
     gene            18316..>18533
                     /locus_tag="VFI55_16745"
     CDS             18316..>18533
                     /locus_tag="VFI55_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HET7075710.1"
                     /translation="MASALFEGPVTSGAVMPIVRVAILADSRLTEMALPVELPLREIL
                     PAVRRLVVPSESNGDGGAPDLGASHLSLA"
BASE COUNT         3100 a         6066 c         6363 g         3004 t
ORIGIN      
        1 ccttggagta gagccgcgac tcctcgatca tgcgctggat cgccgcggga tcggcctcga
       61 gcccgaaatg cggggcgacc cgctgccaca ccgcgttcgg caggtccgca tggtcgatgc
      121 agagccggcc cgcgcgatcg gcggctgcct ccaacatcgc gcccagcgtt cgggccgcga
      181 actcggcacg cgacatcgta ggcaccgcgg ccggatccag gccgaaccgc cgcgcggccc
      241 gttgcggcgc ggcctgcagc cccaaccagc ccggcggctt ggcgagcagc gacgcgagca
      301 ccagggtcgg gtcgcgctgc acccagatcc acggtgtttc ggggaaggcg gcggcgagaa
      361 cggcgcggcg gcgcacgttc cagctggtgc acttgagcac caaccgccgc tcgtcgccgt
      421 gtcggcggcg gccgagcgcc cgcaccatga gccgcacggc gcgggcaagc gctgcctcgt
      481 cgacccgatc gggatcgagc gccaggagag tgttcagcgg cgccggctcg gcgacgacga
      541 caacgccggg gagcgtgccc agcagccggg acaccagcgt ggacccgcac cgcgaaaggt
      601 gaaagatcat gcccgccggg tcgagcgacg gctcgccatc gagagcgacg agcgcgtcga
      661 gcccggtctt gaccagtggc cgggcatggg ggccggacgc ccagcggtcg acggtctggt
      721 cgaagaacgg ttcggtgaag cgctccgccg acaggtcggc ccaatagagg gccggggcgg
      781 gcccggaaaa gtcgaacctg actggggtcc agcgggcgag gtcggtgagc tgcatcagcg
      841 ggcggctatc tggatcagcc gtcggacagc cggacgagga atccgccgac gatcagcgat
      901 cgggccagtt cgatctgctg cttcgcacca agctcgggca gatcccgaac ccgaaacggc
      961 tcggtactgt tcgagatgaa cagcattgcg gctttgtggt tgggaccggc atctatcgac
     1021 aacgtggcaa actgcagcgc aacgccgttg gcgacagcct tcacacgcga gtagagcggt
     1081 tgatacttcc tgaccagtgt ttgtccgtcg attccggcgg cgttcgagat ctttccgaca
     1141 ggtgggcatc ggccgtgacg gaccaagaca tcggccaacg cgtcgaggcc ctgcgcgatg
     1201 gcgccaggat cctcgacggt cctgacagcg tcacgcagga gggcaccgag tttcgcgtgc
     1261 acggccgggt catccagatg ccgtggcggt agctgggcgt tgagacggtc atcgtggaaa
     1321 ctcagcgaat agagcgcccc gatggcaagc gtgagtgcgg tgggtgcgtg gattcccacg
     1381 gtcaaatgga tggacggctc tgcgtctgtg tcggccgcgt gcaccaagcc tcgcggcagg
     1441 tacagcaggt cgccgacctg taagcgcaag tcggtcggcg acggcaggcc gtcggcggga
     1501 accgctttat cacgacgctg catctcgtga ggtggtatct cggtgccgtc gtacaagtgc
     1561 cagatcttcg atccctggat ctgcaggatc aacacgtcgt ggtcgtcgta gtggggaacg
     1621 agccctcgcg atccgggtgg cgtgatgtaa gcgttgacct gtgtcgggaa gttcagctcg
     1681 acctcgacgg aatgggccag cgaagcaatc gcacgcacgt agcgctcaac gccgtccaca
     1741 acgatcgtga agccatcggc aaagtcgttg cgaatgccga cgagatcgag gttgccatcg
     1801 acaagccgat agctatcgga gccccgcttg tctttctccc tcatcaggcg cacagcggac
     1861 ggctcgacgc gataaagctc caagagctcg tccagcgctg acggacccgg cagcaggctg
     1921 tcgaagtaac cggcagagtg ccggtcgaca tgcaagtgcg ttgctgccca gatcttgtcg
     1981 agaaaggctt ccaccgtgat gggcgcaagc agccaagcta gcgacggttc gatggctgac
     2041 ctccccaaac gtcttcctaa gcgcggtggt gaggaggcta gcaacgtaag ctccgcactg
     2101 gctgtgagtc gaatggcgcg aagagagcga cgccacggac atcagcaagg cctagtagcg
     2161 ccagatgtcc gagcgtgctc tccgttgggc cgcaacggtt aatcctcagg gttgaaaagt
     2221 cttcgcggaa ctacgcgaac cctttttccg accaacacag ggcttgatgc gacactccgc
     2281 cggtgaggta tcggggccgg tgaggtaacg gggctgaatc ccggtcggaa gcgggaggct
     2341 tggcgcttgc gattcgacgg aaggggagcg ggctgatgaa ttttcaggtc ttgccgccgg
     2401 agatcaattc gttgctgatg ttcaccggtg cgggctcggc cccgatgttg gcggcagcag
     2461 cggcctggga tgggttggct tccgaattgg gttcggcagc gtcgtcgttt gggtcggtga
     2521 cgtcggtttt ggccgctcag tcgtggcagg gcccggcgtc gacagcgatg ctggctgcgg
     2581 cgtctccgta tgcgggctgg ctcaatgtgg cggcgactca agcccagagg gcggccagcc
     2641 aggccaaggc cgtggtcggt gtgtttgagt cggctcaggc agcaacggtg catcccctgg
     2701 tggtggcggc taatcgcgat gaattcgtgc aattggtgca atcgaacttt ctcgggcaga
     2761 acgcgccggc gattgcggct gccgaggctc ggtacgaaga gatgtgggcc gccgatgtgg
     2821 ccgcgatggc gggttatcac ggcggagcct cggcggcggc tgcgcagttg tcatcgtggc
     2881 agggtgcgtt gccggctctg ccgggtatga gtcaggtggc cagtgcggtg gcaggtagcc
     2941 cggttggagc tgctgcggct gcagtgtcga tggaggcttc cactgttccc ggtctgccgg
     3001 ccctcaacac cgggctgggc aacatcggca gctggaacct gggcggcggc aatattggta
     3061 ataccaacct gggcagcggc aacgtcggca acaccaacct gggcagcggc aacatcggta
     3121 gtgccaacct gggcagcggc aatatcggca acacgaacct tggcagcgga aaccaaggca
     3181 gcaacaactt cggccttggg aacatcggca ataccaacct gggcagcgga aacgtgggca
     3241 gcaataactt tggccttggg aacatcggta atctcaacct aggtggcgga aaccagggca
     3301 gcaacaacgt cggcggcggg aacctcggct ccaacaactt cggtagcgga aacaccggca
     3361 attccaacgt cgggttcgga aatctcggca gcaacgattt cggcttcggc aatagcggca
     3421 gcggggatat cggcatcggg ctcaccggca acaatcagga gggcatcaac ttcgcgggat
     3481 tgctgaactc gggggcagga aacttcggct tcgggaacgc gggtaacaac aacatcggtt
     3541 tcttcaattc cggtgatgga aacttcggca tctttgactc cggcaacggc aattttggtt
     3601 tcgcgaactc aggcaatacc aacacgggtt tctggaatgg ggggagcttc aactctggct
     3661 tcggcaacgc ggctgacctc aacttcggct tcgcgaacgc gggtccgggg aacatgggct
     3721 tcgggaacgc gggtagcggc aacgttggcg tcggtaacgc gggccttttc aactacggca
     3781 atttcaactc gggcggcgtg ctggggggtg cgggcaacac gggtggcttc aactcgggca
     3841 atgtggacac gggctggttt aactcaggta acaccaacac gggtctgctc aactcgggca
     3901 atctggacac gggcgtcggc agctcggctg acctgaccgg gctagtcacc aactcaggct
     3961 tcggcaactc cgggaccgga aactcaggtt tctacaattc gggcgacttt tcctccggct
     4021 tgctgaacaa tggctcgcaa agcgccggct tgttcaacac gggcgatgtg cagacgggca
     4081 tatttaacac gggcggctcg aatactggct tcttcaaccg ggattttgac aacgtcggct
     4141 tcggaaacac tggccagctg aacagcggtt tttccaactc cggcaccggt gacagcggct
     4201 ttgaaaactc aggcaattac gattcgggcg ccctcaaccg gggggactat caggccggta
     4261 ttcttgggaa cggtaccccg gattcgggct gaactttgct gtgatgtcgt gcgcgcagcg
     4321 gaacggaacg tatcctcgcc aacatcccag catgaccaac cggtgtccgc gaccaggcca
     4381 ccggaaggga cgcggcagcc cctcggcgta atgtcgggcg ggtgcgtagc gaaggcgata
     4441 cctgggatat cacgacgagt gttggttcga ccgcgttgtt cgtagccacg gcgcgcgcgc
     4501 tggagataca aaagcccgac ccgctggccg tcgacccgta tgcggagatt ttctgccgcg
     4561 ccgtcggcgg tccccccgcc gacgtgctgg acggaaagga tccagaccac ccactgaaaa
     4621 ccgccgactt cggggagcac ttcgtcaact ttcagggcgc tcgcaccaag tacttcgacg
     4681 aatacttcgg tcgggcagtc gacgcgggtg tgcggcaggt ggtcatcgtg gcggccggac
     4741 tggactcccg cgcttaccgc ctggactggc cggacggcac gacgatcttc gagttggacc
     4801 gtcccgaggt cctcgacttc aagcgtgagg tgctcaccag ccacggcgcc caaccccgcg
     4861 ccgagcgtcg agaaatcgct gtcgatctgc gggaggactg gccacaagcc ttgcgggaca
     4921 ggggctttga taccgccgcg ccgtcggcat ggatcgtcga aggtctactg atttatctcc
     4981 cggccaccgc gcaggagcag ttgttcaccg gcatcgactc actcgccggc cccggcagcc
     5041 atgtcgccat cgaggatggc gccccgatgg ccgccgagga attctccgcc gcggtcgaag
     5101 aagaacgagc ggctgccgcc gaaggcgacg gacgagtgtt tttccagctg gtctacaacg
     5161 agcgtcatgc gccggccacc gaatggttcg gcaagcgggg ctggaccgcg gtcggcaccc
     5221 cgttggtcga ttacctacgc gaaaccgggc ggccggttcc cggaccggac accgaagccg
     5281 gcccgatgat cgcgcgcatc accctggtga gcgccgtcaa ggggtaagcc gcttggatcg
     5341 acccgtgctc atcgattggt ggcgggtgtc gaaaaaatct cgaaccgggc gggcggccgc
     5401 gggaactttt ctgcgtcggt aaccgactac tagccgcgtg ctaattacgc cgcctaccag
     5461 ccaaggcggt cgtcgattcc cccaaggagt ccctgctgtg gcattacggg ttgttgcaca
     5521 cggatttgcc atgtgcgcgt cgtattcggt cgcggacggc ggagcatgac cgcgcccgtg
     5581 tggatggctt cgcccccgga ggtccattcg gccttgctga gcagtggtcc cgggccgggc
     5641 agcctgctga cggcggctgg gtcgtggagt tcgttgagcg cgacctatgc atcggtcgct
     5701 gacgagctca gcgcgatcct ggcggcggtc caggccgggg cgtgggaagg gccgagtgcc
     5761 gagcaatacg tggccgccca cgttccttat ttgatgtggc tgatgcaagc cagcgccgat
     5821 agcgcggcca cagccgccca acacgagact gcggccaccg cctacaccgt cgcacttgcc
     5881 acgatgccga ccttggtgga gttggccgcc aaccatgcga tccacgcggt attggtggcg
     5941 acgaacttct tcgggatcaa tgcgattccg atcgcgctca acgaggccga gtatgcgtgg
     6001 atgtgggtcc aagcggccgt cgcgatggtc cagtatgagt tgacatcttt ctcagcggtg
     6061 acttcggcgc cgcaaacgga aacgccgccc caaatcgtca aacccatcca gagcagcgac
     6121 agcagcgaca ctggcggcag tgtgcaggac atcgtcgacg acgattccgg cgacccatac
     6181 caattgagtt ggtgggtgaa tcgattcttc gaagtctcgg aaaccttaga gcgggacata
     6241 ctcgaattcc cgcagaatcc gtcccaagcg atagcgcagc tggagtccga cgttccggga
     6301 ttggtagctg acgaggttgg ccacgcagcg gaggcttacc aggcgttctt cccggagatc
     6361 aatgcgcttg cgctcaccgt gtcggccttc aaccccgggt tcttgggcgg cttgggcggg
     6421 ctgggcgcgc tggattcgat tcagcccgac gcggttgccg cggcgccggc acccacgccc
     6481 gagaccccca gtgtgccggt ggggagtagt agctctccgg tcctgagtac tgcgccgggt
     6541 ccggcgccgt cgtcggcccc ggcttcagct ccgacgaccg cgccagccca ggcgcctgcg
     6601 accggagctc caccgccacc gccggcggct acggcggggg ccgcctaccc gtacctggtg
     6661 ggcggtccga ccatgggtgc cggcgcggga atcagcagca gtgcccaacg caaggcgctg
     6721 gagcccgaga ccgccgcggc cgccgcggcg gctgcggcat cagcactggc gaagcagagg
     6781 gcccgccggc gccggcgggc ggggatgcga gggtacggcg acgagttcat ggatatgaac
     6841 gtcgaggtcg agccggactg gggcaacccg tcggctgccg ggccggtcgc gtcgaccgtg
     6901 gcttcggatc ggggtgccgg aaagctcggg ttcgccggca cgacacgcaa ggagaccatc
     6961 gccgaggccg ggggactggc cacgctggcc ggtgacgagt tcggcggcgg accgtcggtc
     7021 cccatggttc cgcggacctg ggaccacgac cgagtgcccg gtgaggggga ccggagctga
     7081 acttcgttcg ggagacccac gcgtcctttt gctaatgata atgattttca gtaagctgcg
     7141 ctgcttagtg gagtccgccg aaaaggcgac cgatgatcgc ccagaatgcc caggtcagtg
     7201 ccaccgaaag ggaagccgag ccgtgagccg acggattcta ggaacctcga cagttagctt
     7261 atgcaatgct aacttcaggc gggtcaggtt aggggacact acgtacggga gaagaagctg
     7321 ccgtggaacg cggtgacacg ggcgtgctcg cagctcagca ggtcaattct cggtcggacg
     7381 gcagggtcga cggcgacacc gtcagccggt tcgccacgtg ttgccgtgcg ctgggcatcg
     7441 cggtgtacga gcgccagcgt caggccgacc tcgccgccgc ccgctccggt ttcaccgcgc
     7501 tgacccgcat cgcccacgag cagtgcgacg cctggaccgg gctggcggcc gccggtgacg
     7561 tatccctcag agtgctcgag gccacttcgc gcacggcgac cacggcgggc aagctgcagc
     7621 gcgaggtgga gctggcgccc ggggcgctgg gcttccgcta cgacaccggc ctgtacctgc
     7681 agtttcgggc caccactccc gacgatttcc acctcgccta tgcggcggcg ctggcctcgc
     7741 ccgaggccgg tcggtttgcc gacgccgacc ggatcgtttc cggcctcatc gaacgccggc
     7801 cgagctggcg tgaggcccgc tgggtggccg tcgtcatcaa ctaccgcgcc gaacgctggt
     7861 cggacgtcgt caagctgctg accccgatcg tcaacgacac cgatctcgac gagaccttct
     7921 cccacgccgc caagatcacc ctgggcaccg cgctggcgcg gctgggcatg ttcgccccgg
     7981 cgctctcgta tctcgaggaa cccgagggcc ccgtcgtggt ggcggcggtc gacggcgcac
     8041 tggtcaaagc gctggtgctg cgcgcgcacg tcgacgagga gtcggcgaac gaggtgctgc
     8101 aggatttgta cgccgctcat cccgagaacg aacaggtcga gcaggccctg tccaacacca
     8161 gcttcgggat cgtcacgacc accgcggccc gcatcgatgc ccgcaccgat ccatgggacc
     8221 ccgcgaccga gccgagcgtc gacgacttcg tcgaccccgc ggctcacgaa cgcaaggccg
     8281 tgctgctgca cgaggccgag cgccagctgg ccgaattcat cggcctggac gaggtcaaaa
     8341 accaggtgtc gcggctgaag agctcggtgg ccatggaact ggtgcgtaaa cagcgcggtc
     8401 tcgcggtggc gcagcgcgcg caccacctcg tgttcgccgg cccgcccgga acgggcaaga
     8461 ccaccatcgc ccgcgtggtc gccaaaatct attgcggcct agggcttttg aagcgggaga
     8521 acatccggga agtgcaccgc gccgacctca tcggtcagca catcggcgag accgaggcca
     8581 aaaccaacgc gatcatcgac agcgcgctcg atggcgtgct gttcctcgac gaggcttacg
     8641 cgctggtggc caccggcgcc aagaacgact tcgggctggt ggccatcgac accctgctgg
     8701 cccggatgga aaacgaccgc gaccggctgg tggtcatcat cgccggctac cgggcggacc
     8761 tggacaagtt cctcgacacc aacgagggcc tgcggtcccg gttcacccgc aacatcgact
     8821 tcccctccta cgtgccggcg gagctggtcg agatcgcgca caagatggcc gagcaacgcg
     8881 acagcgtctt cgaacccgcc gcgctcggcc acatggaggc gctgttctcc aaattggccg
     8941 cggagtcgac gcccgacacc aatggaatct cgaggcggag cctcgacatc gccggcaacg
     9001 gccggttcgt ccgcaacatc gtcgaacgct ccgaagaaga acgcgaattc cggctcgatc
     9061 actcggaaca cgcgggaacg ggcgaattca ccgacgagga gctgatgacc atcaccgacc
     9121 aggacgtcga gaagtcagtg gagccgttgc tgcgtggcct cgggttgtcg gtgccgtcat
     9181 gacgaccaac gagccggacc ccgaacgcgg cggagcgcgg cgatcgttct cttcccgaac
     9241 cccggtcaac gacaatcccg accaggtgat ctaccggcgc ggattcgtca ctcgacacca
     9301 agtgacgggc tggcggttcg tgatgcgccg gatcgcttcc ggtatcgcgc tgcacgacac
     9361 caggatgctg gtcgatccgc tgcgcaccca gtcgcgcgcg gtgctgatgg gcgcgctcat
     9421 cctgatcacc ggcctggcgg gctgcttcgt gttctcgctg atccggccca gtgggcaggc
     9481 gggcaacaac gccgtgctcg ccgaccggtc gacggacgcg ctctatgtcc gggtgggcga
     9541 ccagctgcac ccggtgctca acctgacctc ggcgcgcttg atcgtcggtc gtcccgtcga
     9601 cccgaccgcg gtgaaaagca gtgagctgga ccgctttccg cgcggcaacc tgatcggcat
     9661 tcccggcgcg ccggagcgga tggtgcagaa cacctcgcac gacgcgaact ggacggtgtg
     9721 cgacgcggtc agcgggagcg gtcagggggg gcacggcgcc catagcaccg gcgtgaccgt
     9781 gatagcgggc ccgcccgaca gccagggcgc gcgggcggcc aagctcggat ccggacaggc
     9841 tgtcctggtc gacttgtccg gtgacatggg caccagcacc tggctgttgt gggacggcag
     9901 gcgcagccag gtcgacctgg ccaaccacgc ggtcaccagc gcgctgggcc tgggagccgg
     9961 tgtgcccgcg ccgcggccga tcgcattcgg gctgttcaac gcgatcccgg aagggccgcc
    10021 gctggcggcg ccgaccatcc ccaacgccgg tgcgccggcg aacttttccg tgccggctcc
    10081 gatcggggcc gtggtggctt cctatgggtt ggaccagaat tcgtcgggcg gggtgcgcta
    10141 ttacgcggtg ctcccggacg ggctgcagcc catctcgccg gtgctcgccg cgatcctgcg
    10201 taacaccaat tcctatgggc tggaccagcc gccgcggctc ggcgcggacc agattgccaa
    10261 gctgccggtg tcgcggatgc tggacaccgc gcgctacccg gaccagcagg tcgcattggt
    10321 cgacgcgacc aaggaccccg tcacctgtgc gtcctggagc aggccggccg gggccgccag
    10381 cagctcaatg agcctgctgt ccggctcggc gttgccggtg cccgacgcgg tacacaccgt
    10441 cgacctggtc ggcgccggct accagggcgt cgccagccgc gtcgccctgg cgccgggcac
    10501 cggctatttc gcccagaccg tcgacggcga cgcggcctca ccgggcgccg ggtccctgtt
    10561 ctgggtgtcc gacaccgggg tgcgctacgg catcgacact gagactggcg aggcggccaa
    10621 tcccgccggc ggccatggca aacccgttga ggcccttggc ctgagcgcgc cgccggtccc
    10681 cgtcccctgg tcgatcctgt cactgttcgc gaccggcccg gcgctgtcgc gtgccgacgc
    10741 cctgctcgcc cacgacggcc tggcgcccga cagcaggccc ggtcgcccgg tcgcggccga
    10801 aggagcgccc cgatgagccg actgattttc gaggcccgcc ggcggctggc gccaccggcc
    10861 gtccgcaaag gcaccatcac catcgaggca ccgcccgagc tgccccgggt gattccgccg
    10921 tcgctgctgc ggcgcgcgat gccgtacctg atcgtgatcc tgatcatcgg catggtggtt
    10981 gcgctggtcg ccacgggcat gcgggtgatc tccccgcaga ccctgttctt cccgttcgtg
    11041 ctgctgctgg cggccaccgc gctgtatcgc ggcaatgaca acaagatgcg caccgaggag
    11101 gtcgacgccg aacgggccga ctatctgcgt tacctgtcgg tggtgcggga caacattcgc
    11161 acccaggccg ccgagcagcg cgccgccgcg cagtggtccc accccgagcc gacggagttg
    11221 gcgacggtgc cgggctcgcg ccgtcagtgg gagcgtgacc cgcacgaccc cgacttcctg
    11281 gtgctgcgcg ccggccgtca ccaggtgccg ctggccaccg cgctgcgggt caacgacgcc
    11341 gccgacgaga tcgacctgga accggtgtcg cacagcgcat tacgcagcct gctcgacacc
    11401 cagcgcaccg tccccgacgt gccgaccgga atcgatctga ccaaggtctc gcggatcacg
    11461 gtcctgggtg agccagatga ggtgcgcggt gcactgcgcg cctggatcgc tcaggcggtg
    11521 acgtggcacg acccgacggt actcggggtg gcgctggcca cccgcaactt ggagggcccg
    11581 gactggtcct ggctgaagtg gttgccgcac atcgacattc ccggcgaact cgacggtgtc
    11641 ggcccggccc gattccttgc ggccaacgcc gacgacctgg tcgcgctgct ggaacccgcg
    11701 ttggtcgaac gcccggcgtt caccggcgga cgctccgatg cgctgcggca tttgctgatc
    11761 gtggtggacg accccgacta cgacctgaac gcctcggcgc tcgcggtcgg ccgggcgggc
    11821 gtcacggtgg tgcaccgctc ggccaaaccc ccgcaccgcg aacagtattc ggatcccgag
    11881 aagccgatcc tgcgcatcgc gcacggcgcc atcgagcgct ggcagaccgg cggctggcag
    11941 ccctatatcg acgccgccga cgacctgggg cccgacgacg cctcccacct ggcccggcag
    12001 ctgtcgcggt gggactccaa cccgacgcac gccgggctgc gctcggcggc cacccgcggc
    12061 gccagtttca ccacgctgct gggcatcccg gacgcgtcgc agctggacgt gcccacgctg
    12121 tgggcgccgc gccgccgcga cgacgagttg cgcgtaccga tcggtgtgac ggcgaccggc
    12181 gagccgctga tcttcgacct caaggacgaa gccgagggcg gaatgggtcc gcacggcctg
    12241 atgatcggta tgacggggtc cgggaagtcg cagaccctga tgtcgatcct gttgtcgctg
    12301 ttgactactc attcggcgga ccggttgatc gtcatctacg ccgacttcaa gggcgaggcc
    12361 ggcgccgaca gcttccgcaa cttcccgcag gttgtcgcgg tgatctcgaa catggccgag
    12421 aaaaagtcac tggccgaccg gttcgccgac acgctgcgcg gtgaggtggc ccgccgcgag
    12481 acgctgctgc gcgaagccgg ccgccaggtt cagggcagtg cgttcaactc ggtgaccgag
    12541 tacgagaacg ctcgcgaatc cggggcagcg ggcgcagtcg atctgccgcc gattccaacg
    12601 ctgttcgtgg tcgccgacga gttcaccctg atgctggccg atcacccgga atacgcggag
    12661 ctgttcgact acgtggcgcg caagggccgc tcgttccgca tccatatcct gttcgcgtcc
    12721 cagacgctgg atgtgggcaa gatcaaggac atcgacaaga acacctccta ccgcatcggg
    12781 ttgaaggtgg ccagtcccag cgtctctcgc cagatcatcg gcgtcgagga tgcctatcac
    12841 atcgagtccg gcaaagagca caagggcttg ggcttcctgg tgcccgcccc gggcgcgacc
    12901 ccgatcaaat tccgcagcac ctacgtcgac ggcatctacg atccgccgca ggcggccaaa
    12961 tccattgtgg tgcagtcaat tttagagccg aagctgttca gcgccgggcg ggtggagccg
    13021 gaccaggaca cgctgatctc cagcgccggc gaagaggaaa cggccggacc gccgcgcaag
    13081 ctcatcgcga ccatcggcga gcaactggag cgctacgggc cgcgggcacc gcagttgtgg
    13141 ctgccgccgc tcgatgaacc gattccgctg accgcggtgc tggcgcgcgc cgggataccg
    13201 cgacaggagt ggcggtggcc gctcggtgag atcgacaagc cattcgagat gcgccgcgac
    13261 ccgctggtgt tcgacgccac gtcgtcggcc ggcaacgtgg tcattcacgg cggcgccaag
    13321 tccgggaaat cgactgcgct gcaaacgttc atcctgtcgg cggccagcct gcactcgccc
    13381 cgcgacgtca cgttctactg cctcgactat ggcggcgggc agttgcgggc ggtggaagat
    13441 ctggcacacg tcggcagcgt cgcctcgggg ctggagcccg agcgcatccg ccgcaccttc
    13501 ggtgagctgg agcagctgtt gctgtcccgg ctgcggcggg aggtattcaa ggacagaaat
    13561 ggttcggccc ccgacgacgg cttcggcgag gtgttcctgg tcatcgataa cctctacgcg
    13621 ttcggccgtg acaacaccga ccagttcaac acccgtaacc cgttgctggc caaggtgact
    13681 gagctggtca acgtcggcct ggcgtacggc atccacgtga tcgtcaccac ccccagctgg
    13741 ctggaggtac cgctggcgat gcgcgacggc ctcgggctgc ggctcgagct caagctgcac
    13801 gatccgcggg acagcaacgt gcgggtgatc ggcgccctgc gtcgtccggc cgacgcggtg
    13861 ccgcacgacc agccgggccg cgggctgacg atggcggcgg agcatttcct gttcgcggct
    13921 ccggagctcg atcaggtggg ggcgatcaac gcccgctacc ccggcacggc agcgccgccg
    13981 gttcggctgc tgcccgtcaa cctggccgcc ggcgccgtca ggccgctcta ccggggcccc
    14041 gagcaggtgg tcatcggcca gcgcgaagag gatctcgcgc ccgtcgtcct cgacttcacc
    14101 gagaaccccc tgttgatggc gttcggcgac agcaagtcgg gtaagaccac gctgctacgc
    14161 cacatcgttc gcactatccg cgagcactcc accgaggacc agatcgcgtt caccgtgctg
    14221 gatcgccggc tgcacctggt ggacgagccg ttgttccccg acaacgagta caccgccaac
    14281 atcgaccgga tcctgccggc gatgctcggt ttgtccaacc tcatcgagtc acggcggcca
    14341 ccggcgggat tgtcggccgc cgaactgtcc cgctggacgt atcagggcca cacgcattac
    14401 ctgatcatcg acgacgtcga ccagataccg gattcgccgg tgacgagcgg tccctacatc
    14461 gggcagcggc cctggactcc cctcatcggg cagctctcgc aagccgccga tctggggctg
    14521 cgggtgatcg tcacggcgcg cgccagcggg tccgggcaca cgttgatgac cagcccgttg
    14581 ctgcgccggt tcaacgacct gcaggcgacc accttgatgc tttcgggcaa cccgcaagac
    14641 agcggcaaga tccggggcca gcgattcaac cggttgcctg ccggtcgggc gatcctgttg
    14701 ggcgacagcg acagcccgac ttacgtgcag ttggtcaacc cgctcatcgg ggagtccgtg
    14761 ctggcaagtg aagcgcaagc agagggacaa cagaaggagt ggccatgacg ttgcgagtgc
    14821 ttcccgaggg cctggccgcc accagcgcgg cggtggaggc gttgacggcg cggctggcgg
    14881 ccgcgcacgc ggcggcggcc ccggcgatta ccgcggtggt gcccccggcg gcggatccgg
    14941 tgtcgctgca gacggccgcc ggcttcagcg cccagggcca ggagcacgcc gccatcgcgg
    15001 cacagggtgt cgaagagctg ggccgcgccg gtgtgggtgt gggtcaggcc ggggcaagct
    15061 acctcgccgg tgacgccgcg gccgccgcta cctatgggat ggcaggcggc tgacaatgac
    15121 tggcccgttg gtcccctttg ggtccatcgt gtggatggct tcgccgccgg aagtgcattc
    15181 ggcgttgctg agcaccggac cgggaccggg gtcgctgttg gcggccgccg gcgcctggtc
    15241 ctcgctcagc accgaatacg cttcggcggc agccgaactc agcgggctcc tgggggcggt
    15301 tcaggcgggg gcgtggcagg ggccgactgc cgagcggtat gcggcggctc acctgccgta
    15361 cctggagtgg ttgcagcagg ccagcgctga cagcgccggt gtcgccgcac agcacgaggt
    15421 cgcggcgacc gcgtacaccg ccgccgtggc ggcgatgccg acattggcgg aattggcggc
    15481 caaccacgtc gtgcatgggg tgctggtagc gacgaatttc tttgggctca acacgattcc
    15541 gatcgcgctc aatgaggccg actatgtgcg gatgtgggtc caggccgcca ccacgatggc
    15601 gggttaccag gccgtcgcgg gcgcggcgct ggcgtcggcg ccgcgcagca ttccggcgcc
    15661 gaatgtggtg aaccccggtg tcggtgaaac cggcaacgcg gcggccgcta ccacccaggg
    15721 cggcgcggcc acccaggcgg cccagtccgg ctcgtccctg aacctctcgg acctgctgtc
    15781 gcagctgctg tcggagtaca ccaatttcta cggggggatg tatcaggagc tcggttcgtt
    15841 cctccagaat cccatcggaa attcggtgca gctcatcgag gcgttcttga cgaatccgtc
    15901 tgaggcgctg gtgctctacg gtccgctgtt gtttgcgatc gcctggcagg ccttctcctg
    15961 ggtcggtgcg tcgatcacgt atccgtcgct gtttctcagc cccttcctgg ggctcgtccg
    16021 gccgctgacg ttgcccctgg cgctggcgaa tgcggtcgaa gtggcgccga cgttgacaga
    16081 acccgtcgcg ggcggcgtcg cggtgaccgc ccaaccggta tcggtacagc cggtggtggc
    16141 gctggcccca acggccgcgc ccgcggcggg gatcccggcg tcgacgcccg cgggtgcggg
    16201 tgcggccgct acacccgccc cgggggcggc taccggcagt ttcgcctatg tggtgggcgg
    16261 cggcggtaat ccagaaactg gctttgggcc gacgttgacc gaccgtggtg ggaccaaggc
    16321 accggcggcc accattcccg cggccgccgc ggcggtaccg agtgccgcgc agtcccgggc
    16381 ccgccggcgc cggcgggcgg caatgcgcga gtacggcgat gaattcttgg acatggattc
    16441 cgacatcggc gttgcgcccg actacggcca acccgagcag ctggtctccg ccatggcttc
    16501 cggaaatggc gccggcacgt tgggctttgc cggcacggcg cacagagaag cggctttgcc
    16561 ggccgcggga ttgaccaaac tggccggcga cgaatacggc ggcgggccgc ggatgccgat
    16621 gctgccgggc acgtgggaac aggacaacca cggcgctgaa ccggggagag ggggctcaag
    16681 caattagcac gatgctcagc aatgcgccag tgcaccgaaa ttcgaaaggg aaggaatcgt
    16741 tatgagtctg ttggacgcgc atattccgca gttgatggcc gcgcagtcgg cgttcagcgc
    16801 caaggcggcg ttgatgcgca gcacgatcag ccaggccgag caggaagcgc tgtcggcgca
    16861 gtcgtttcac gtgggggagg cgtcggcggc gtttcaggcc gcgcacgccc gctttgtgga
    16921 agccgcgagc cggatcaacg cgctgctgga catcgcccag gccaaccttg gggacgccgc
    16981 gggcacctat gtggccgccg acgccgcggc cgcttccggc tacacggcgt tctgaccaga
    17041 ccgctactaa ccgcgaaggg acttgcgatg tcagagatca tgtacaacta cccggcgatg
    17101 ttgggtcacg cgggggacat gtcgggctac gccggcacgc tgcaggggct gggcgccgat
    17161 atcgccagcg agcaggccgc gctgtctaat gcctggcagg gcgataccgg catgacctat
    17221 caggtgtggc aggcccagtg gaaccaggcg atggaatctc tggtgcgggc ctaccagtcg
    17281 atggccagca cgcacgaggc caacacgatg gccatgtacg cccgtgacgc cgccgaagcc
    17341 gccaaatggg gcggctagtt cccgccgaga cgacgtaaat gaacgcagaa cccaacgccg
    17401 tcgagctgac ggtcgatcaa gcgtggttca tcgcggaaac cattggggcg ggcagcttcc
    17461 cgtgggtgct ggcgatcacc gcgccctatt gtgatgccgc gcaccgggag gcgttctgcg
    17521 aacgtcagcg gtccgaactg atccggatgg gtctgttgtc gcaaaccggg ctcctcaatc
    17581 cggccgtcgc cgactggatc cgggtggtgt gctttccgga ccggtggctc gacctgcgtt
    17641 acgtggggca cgccccagcc accggctcgg gcccggctac cgacgacctg ctgcgcggcg
    17701 tcgtcgcgca gcgcgtcggc gccgccgatc gggccgccaa gacccgtaag acggtggtcg
    17761 cgttgcgcag cgcgcagctg atcacgttta ccgccatgga tatcgacgac gcccgggcgc
    17821 tggttcccgt cctcggtgtt gggttggcgc agcggccgcc ggcgcgattc gacgcgttca
    17881 gcatgcccac gcgcgtcggc gcccgggccg acgagcggct gcggtccggc gttgcgctgg
    17941 ccgaagtcgt tgactatctg ggcatcccgg catcggcgcg accggtggtg gagtcggtgt
    18001 tctgcggtcc gcgcagctac gtcgagatcg tcgccggctg ccgccgcgac gggcggcact
    18061 ccaccaccga ggtcggcttg agcgtcgtgg acagcgccgc gggccgggtg ctggtcagcc
    18121 cgtcccgcgc cttcgacggg gagtgggtct cgacgttcag cccggggacc ccgttcgccg
    18181 tcgccgtcgc gatcgaacaa ctgaccgccg aactgcctga cggccaatgg ttccccggcc
    18241 agcggctggc ccgagatttt tctgcccaac acgcataact gaaacccgat agaaagagag
    18301 cacgatgtcc caggaatggc ctcagcactt ttcgagggcc cggtgacgtc cggcgccgtc
    18361 atgccgatcg tccgcgttgc cattctcgcg gacagcaggt tgacggagat ggccctgccc
    18421 gtcgagttgc cgctgcgcga aatactgcct gcagtgcggc gtttggtggt cccctccgag
    18481 tcgaacggtg acggcggtgc gcctgacctc ggggcttccc acctgagtct ggc
//
LOCUS       DASSGQ010000380         6454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_333141,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000380 DASSGQ010000000
VERSION     DASSGQ010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6454
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333141"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..857
                     /locus_tag="VFI55_16750"
     CDS             132..857
                     /locus_tag="VFI55_16750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HET7075711.1"
                     /translation="MSPRRWLRAAAVIGATAMLLASSCTWQLSLFIPEGVPPPAGDPV
                     PPVNTHASGRPADQLRGWALPRSVALEIPVIALEAYAYAARVAEVENPKCHIAWTTLA
                     GIGQVESHHGTYRGAKLAPNGDVSPPIRGVRLDGSGGTLRIVDTADGDGAADVVRAMG
                     PMQFIPETWRLYGVDAHNDGVVSPDNIDDAALAAAGYLCWRGKDLATARGWITALRAY
                     NNSGVYARAVRDWATAYAAGHPL"
     gene            936..2225
                     /gene="eno"
                     /locus_tag="VFI55_16755"
     CDS             936..2225
                     /gene="eno"
                     /locus_tag="VFI55_16755"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET7075712.1"
                     /translation="MPIIEQVGAREILDSRGNPTVEVEIALIDGTFARAAVPSGASTG
                     EHEAVELRDGGRRYGGKGVQKAVQAVLDEIGPAVIGLNADDQRLVDQALVDLDGTPDK
                     SRLGANAILGVSLAVAKAAADSADLPLFRYIGGPNAHIMPVPMMNIVNGGAHADTGVD
                     IQEFMVAPIGAPSFAEALRWGAEVYHALKSVLKSQGLSTGLGDEGGFAPDVAGTTAAL
                     DLIGRAIEKAGFKPGVDVALALDAAATEFYTEGTGYTFEGSTRTAEQMTEFYAGLLGA
                     YPLVSLEDPLAEDDWEGWAALTAAIGDRVQIVGDDIFVTNPERLEEGIEKGVANALLV
                     KVNQIGTLTETLDAVALAHHSGYRTMMSHRSGETEDTTIADLAVAVGSGQIKTGAPAR
                     SERVAKYNQLLRIEEALGDAARYAGDLAFPRYALDSK"
     gene            2261..2962
                     /locus_tag="VFI55_16760"
     CDS             2261..2962
                     /locus_tag="VFI55_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET7075713.1"
                     /translation="MPEAKRPDSKRRSPASRPGKAGDAIRRPRATKPSKPSQTANHAT
                     RNPTRTVQERVVEPIKRQVAESVEQRSDQRLGFTARRAAVLAALICVLTLTIAGPVRT
                     YFAQRTEMDQLAASEAALRRQIADLEQKKVKLGDPAYIAAQARERLGFVMPGDIPFQV
                     QLPPTAASPQPGGAEAAKPANNDPWYTSLWHTIADAPHLPPANAPSPQVPPPGEPGSS
                     GPVPPDAPNPTAPGG"
     gene            2955..3455
                     /locus_tag="VFI55_16765"
     CDS             2955..3455
                     /locus_tag="VFI55_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003901072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HET7075714.1"
                     /translation="MVDRADLEAVARQLGREARGVLEIAYRCPNGEPGVVKTAPKLPD
                     GTPFPTLYYLTHPVLTAAASRLETTGLMGEMTERLRQDPELAAAYRRAHDSYLAERDA
                     IEPLGTTFSAGGMPDRVKCLHVLIAHSLAKGPGVNPLGDEALAILVREPRFKSDASGL
                     VGEPWR"
     gene            3446..4390
                     /locus_tag="VFI55_16770"
     CDS             3446..4390
                     /locus_tag="VFI55_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020786178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HET7075715.1"
                     /translation="MAVRAAAIDCGTNSIRLLIADASDGQLRDLHRETRIVRLGQGVD
                     ATGQFAPEAIARARAALADYASLLSRHGVRRVRMVATSAARDVTNRADFFAMTAEVLG
                     AVIPGAVAEVITGAEEAELSFRGAVGELDKAAAPFVVVDLGGGSTEIVLGSDRVVASY
                     SADIGCVRLTERCLHSDPPTPEEVQAARQVVRERLDVALGVVPVEGARTWVGLAGTMT
                     TLSALAHNMTAYDAAAIHLSRVSGGDLLAVCDRLIGMTRSQRAALGPMHEGRADVIGG
                     GAIVVEELARELRARAGIDELTVSEHDILDGIVLSIAG"
     gene            4493..4786
                     /locus_tag="VFI55_16775"
     CDS             4493..4786
                     /locus_tag="VFI55_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011742165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075716.1"
                     /translation="MNQPLRGIELRYVLTMHLAVHGPATITEMIDALGWHGFCVPGRV
                     SKVVSDALRWEIGRGRVRRLGRGLYGPGRIPRATEYRIHQRVLALRAAATLSL"
     gene            complement(4839..5519)
                     /locus_tag="VFI55_16780"
     CDS             complement(4839..5519)
                     /locus_tag="VFI55_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7075717.1"
                     /translation="MTRVLVIDDEPHILRALRINLSVRGYEVVTASTGAGALRAAAEQ
                     KPDVVILDLGLPDISGIEVLAGLRGWLTAPVIVLSARTDSSDKVEALDAGADDYVTKP
                     FGMDEFLARLRAAVRRNATASEVDQPVVETEAFTVDLAAKKVTRDGVEVHLTPTEWGM
                     LEVLVRNRGKLVGREELLKEVWGPAYATETHYLRVYLAQLRRKLEDDPSHPKHLLTES
                     GMGYRFEA"
     gene            complement(5516..>6454)
                     /locus_tag="VFI55_16785"
     CDS             complement(5516..>6454)
                     /locus_tag="VFI55_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7075718.1"
                     /translation="NGGKPYVVACVGNEPCVTVDTADTAIEVGDDEFWMLLAGKKLSA
                     RDRRVLGAVANQAAGLVKQRELAEEAGRTQAIVQADELRRSLLSAVSHDLRTPLAAAK
                     VAVSSLRAEDIAFSPEDTADLLATIEESIDQLTALVGNLLDSSRLAAGVVRPELRRVY
                     VEETVQRALVSIGKGATGFYRSAIDRVKVDVGDAVVMADPGLLERVLANLIDNALRYA
                     PNCVVRVNAGRVGDRVLINVIDEGPGIPKGSEDQIFEAFQRLGDHDNTTGVGLGMSVA
                     RGFVEAMGGTIAAGDTPGGGLTVVVDLAAPQPVGVR"
BASE COUNT         1061 a         2164 c         2227 g         1002 t
ORIGIN      
        1 agctaatcgc taattcggcg aaatccgggc cgcctcggtg cacgcggcgc ccaatgtcgc
       61 cggttagtcc cggaacccat gggacgatgg tcgtggccga agtgtgtgca ggggagctcg
      121 aaggaaacat ggtgtcgccg agacgttggt tgcgtgcggc cgcggtgatc ggcgcgaccg
      181 cgatgcttct ggcctccagc tgcacctggc agctcagcct gttcatcccc gagggcgtgc
      241 cgccgccggc cggagatccg gtgccgccgg tgaacactca cgccagcggc cggcccgctg
      301 atcagctgcg cggatgggcc ctgccacggt cggtggcgct ggagattccg gtcatcgcac
      361 tggaggccta cgcctacgcc gcccgggtcg ccgaggtcga gaacccgaag tgccatatcg
      421 cgtggaccac gctggcgggc atcggccagg tcgagagcca ccacggcacc taccggggcg
      481 cgaagctggc gccgaacgga gatgtgagtc ctcccattcg gggcgttcgc ctggacggca
      541 gcggcggcac cctgcgcatt gtcgacaccg cggacgggga tggggccgcc gacgtggtgc
      601 gagcgatggg accgatgcag ttcattccgg agacgtggcg gttgtacggc gttgacgcgc
      661 acaacgacgg cgtggtcagc ccggacaaca tcgacgacgc ggcgctggcc gcggcgggct
      721 atctgtgctg gcgcggcaaa gacctggcga cggcgcgcgg gtggataacc gcgctgcgcg
      781 cctacaacaa ctccggcgtc tacgcgcggg cggtgcggga ctgggcgacc gcctacgcgg
      841 cgggtcaccc gctgtagcag gatgtatcgc tgacccaggc tttacgctga agacggcctc
      901 ggggccgtca ccagctacga cacaaggaga acccagtgcc gattatcgag caggtcggag
      961 cccgcgagat cctcgactcc cggggtaacc cgacggtcga ggtcgagatc gccctgatcg
     1021 acgggacgtt tgcccgcgcg gcggtgccgt ccggcgcgtc cacgggcgag cacgaggccg
     1081 tcgagttgcg cgacggcgga aggcgttacg gcggcaaggg cgtgcaaaag gccgtgcaag
     1141 ccgtgcttga cgagatcggt ccggccgtga tcgggctcaa cgccgacgat cagcggttgg
     1201 tggaccaggc gctggtggac ctcgacggca cccccgacaa gtcacggctg ggtgccaacg
     1261 ccatcctggg tgtctccctg gcggtcgcga aagcggccgc cgattccgcc gatctgccgc
     1321 tgttccgcta tatcggcggg ccgaacgcgc acatcatgcc ggtgccgatg atgaacatcg
     1381 tcaacggtgg tgcccacgcc gacaccgggg tggacatcca ggagttcatg gtggcgccga
     1441 tcggcgcgcc cagcttcgcc gaggcgttgc gctggggcgc cgaggtgtac cacgcgctca
     1501 aatcggtgct gaaaagtcag gggctgagca ccggcctggg cgacgagggc ggcttcgccc
     1561 ccgacgtggc cggcaccacc gcggcgctgg atctgatcgg ccgggccatc gaaaaggcgg
     1621 gtttcaaacc cggcgtcgac gtggcattgg cgctcgacgc ggccgccacc gagttctaca
     1681 ccgagggcac cggctacacc ttcgagggca gcacccgcac cgccgagcag atgaccgagt
     1741 tctacgcggg cctgctcggc gcctatccgt tggtctccct cgaagacccg ctggccgaag
     1801 acgattggga ggggtgggca gctctgaccg cggcgatcgg tgaccgcgtg caaatcgtgg
     1861 gcgacgacat cttcgtcacc aatcccgaac ggctcgaaga aggtatcgaa aagggtgtgg
     1921 caaacgcgtt gctggtcaag gtgaatcaga tcggcacgct gaccgagacg ctcgacgccg
     1981 tcgcgctcgc ccaccacagc gggtatcgca cgatgatgag ccaccgtagc ggcgagacgg
     2041 aggacaccac aatcgccgac ctggcggtgg ctgtcggcag cggtcagatc aagaccggcg
     2101 cgcccgcccg cagtgaacgg gtcgccaaat acaatcagct gctgcgcatc gaggaggccc
     2161 tcggcgacgc cgcgcgctac gccggtgatc tggcctttcc gcgatacgct ctggactcca
     2221 aatagcttga cggccaacgg aagacgcggc tagatcgcca atgcccgaag cgaaacgccc
     2281 agattcgaag cgccgctccc cggcatcgcg tccggggaag gccggcgatg cgatccggcg
     2341 ccctcgcgca accaagccgt ccaaaccgtc gcaaaccgcc aaccacgcca ccagaaaccc
     2401 gacgcgcacg gtgcaggagc gggtcgtcga gcccatcaag cggcaggttg ccgaatccgt
     2461 cgagcagcgt tccgaccagc ggctgggttt cacggcgcga cgggcagcgg tgctggccgc
     2521 gctgatctgc gtcctgacgt tgaccatcgc cggcccggta cgcacctatt tcgcgcagcg
     2581 caccgagatg gatcagttgg cggcaagcga agccgcgctg cgccgtcaga tcgccgacct
     2641 ggagcagaag aaagtcaagc tcggcgaccc ggcctacatt gccgcccagg cccgcgagcg
     2701 ccttggcttc gtgatgcccg gggacatccc gttccaggtt caactcccgc ccacggccgc
     2761 gtcgccacaa cccggggggg ccgaggcggc aaaaccggcc aataacgacc cgtggtacac
     2821 ctcgctgtgg cacaccatcg cggatgcccc gcacctgccg ccggccaacg cgccttcgcc
     2881 gcaggtcccg ccgccggggg aacccgggtc atccggtccg gtgccgccgg acgcaccgaa
     2941 tcccacggct cccggtggtt gatcgtgccg acctggaagc ggtggcgcgc caactcgggc
     3001 gtgaggcccg cggggtgctc gagatcgcct atcggtgtcc caacggtgaa cccggcgtcg
     3061 tgaagaccgc gccgaagctt cctgacggca cgccctttcc gacgttgtac tacctgacgc
     3121 atcccgtgct gaccgcggcc gcgagcaggc tggagaccac gggattgatg ggggagatga
     3181 ccgaacggtt gcggcaggac cccgaactgg ccgccgccta tcggcgagcc cacgactcgt
     3241 atctggccga acgcgatgcg atcgagccgc tggggaccac gttttccgcc ggaggaatgc
     3301 ccgaccgggt caagtgcctg cacgtgttga tcgcgcattc gctggccaag ggcccgggtg
     3361 tgaatccgct tggcgacgag gcattggcga ttctggtccg cgagccgcgc ttcaagtcag
     3421 acgcctccgg actggtgggt gaaccgtggc ggtgagggcg gccgcgatcg attgcggcac
     3481 caactcgatc cggttgctga tcgccgacgc gagcgatggg cagctacgcg atctgcatcg
     3541 cgagacgcgg atcgtgcgcc ttggtcaggg cgtcgacgcg acgggtcagt ttgcgccgga
     3601 agcgattgcc cgagcccggg ccgcgctggc cgactacgct tcgctgctga gccggcacgg
     3661 tgtacggcgg gtgcggatgg tcgccacgtc cgcggcccgg gatgtcacca atcgcgccga
     3721 tttctttgcg atgacggctg aggtgctggg cgccgtgata cccggcgcgg tcgcggaggt
     3781 catcaccggc gccgaagagg ccgagctgtc gttccgcggg gccgtcggcg aattggacaa
     3841 ggccgcagca cctttcgtcg tcgtcgacct gggcggcggc tccaccgaga tcgtgctcgg
     3901 cagcgaccgg gtggtggcca gctactcggc cgacatcggc tgcgtccggt tgaccgaacg
     3961 ctgtttgcac tccgaccccc cgacaccgga ggaagtccag gccgcccgcc aggtggtgcg
     4021 cgagcggctc gacgtcgcgc tgggcgtggt acccgtcgag ggcgcccgaa cctgggtcgg
     4081 gctcgccgga acgatgacca cgctgtccgc gctggcgcac aacatgacgg cgtatgacgc
     4141 tgcggccatt catctttcgc gtgtcagcgg cggcgacctg ctggcagtgt gtgaccggct
     4201 gatcggcatg acgcgatccc agcgcgccgc gctggggccg atgcatgagg gccgggccga
     4261 cgtcatcggc ggtggcgcga tcgtggtcga ggagttggcg cgcgagctgc gcgcccgcgc
     4321 cggcatcgac gagctaaccg tcagcgagca cgacatcctg gacggcatcg tgctgtcgat
     4381 cgcgggctga accacatacg ccacacgcgt atctcggcaa gtcgacacac tttttgcccg
     4441 cctcacagcg acaacgcctg tcgctgtcgt accgccatgg gattgttcga cgatgaacca
     4501 accattgcgg gggatcgagc ttcgttacgt gctcacgatg cacctggccg tgcacgggcc
     4561 ggcgaccatc accgagatga tcgatgcgct tggctggcac ggcttttgcg tccctggcag
     4621 ggtatcgaag gtggtgtcgg atgcgttgcg gtgggagatc gggcgcggca gggtgcgccg
     4681 gctgggacgc ggactgtacg gcccgggccg gataccccgc gccaccgaat atcggatcca
     4741 ccagcgcgtg ctggccttgc gcgcggcagc gacgttgtcg ctgtgaggcg ggcaaaaagt
     4801 gtgtcgactt gcggagaggc ccgtgtggcg tatgtgactc aggcctcgaa gcggtagccc
     4861 atgcccgatt cggtcagcag atgcttggga tgtgacgggt cgtcttcgag cttgcgtcgc
     4921 agctgcgcca gatacactcg caggtaatgg gtttcggtgg catacgcggg accccacacc
     4981 tccttgagga gttcctcgcg gccgaccagc ttgccccgat tgcgcaccag cacctcgagc
     5041 atgccccact cggtcggagt gaggtggacc tcgacaccgt cccgggtgac cttcttggcg
     5101 gccaggtcga cggtgaacgc ctcggtttcg acgaccggct ggtcgacttc ggacgccgtg
     5161 gcgttgcggc ggaccgccgc gcgcagccgg gccagaaact cgtccatccc aaagggtttc
     5221 gtgacgtagt catcggcgcc ggcgtccagg gcctcgacct tgtccgacga atcggtgcgc
     5281 gccgacaaca cgatcaccgg tgccgtgagc cagccgcgca gaccggccag cacctcgatg
     5341 cccgatatgt cgggcagccc gaggtcgagg atcaccacgt caggcttctg ctcggcggcg
     5401 gcgcgcagcg ctccggcacc ggtcgacgcg gtgaccacct catagccccg gacggacagg
     5461 ttgatgcgca acgcgcgcag gatgtgcggc tcatcgtcga tcaccaacac gcgagtcatc
     5521 gcacaccgac cggttgtggc gcagccagat ccaccacgac ggtaagtcca ccgcccgggg
     5581 tgtcgccggc cgcgatggtg ccgcccattg cttcgacaaa gccccgcgcc accgacattc
     5641 ccaaacccac cccggtggtg ttgtcgtgat cgccgagtcg ctggaaggcc tcgaagatct
     5701 ggtcctcgga acctttcggg atgcccggac cttcgtcgat gacgttgatc agaacgcggt
     5761 cgcccacccg tcccgcgttg acccgaacca cgcaattggg cgcgtaccgc agcgcgttgt
     5821 cgatcagatt cgccaagact ctttcgagca gcccggggtc ggccatcacc acggcgtcgc
     5881 cgacgtcgac cttgacgcga tcgatggcgg atcggtagaa accggtggcg cccttgccga
     5941 tgctgaccag cgcgcgttgc accgtctcct ccacatacac ccgtcgcagt tccgggcgca
     6001 ccacgccggc ggccagacgc gaggaatcga gcaggtttcc gaccagagcg gtcagctggt
     6061 cgatcgactc ctcgatggtg gccagcaagt cggcggtgtc ctccggggag aacgcgatgt
     6121 cttcggcgcg caagctcgac acggcgacct tggccgccgc cagcggcgtg cgcagatcgt
     6181 ggctgaccgc ggacagcagc gagcggcgca gttcgtctgc ctgcacgatc gcctgggtgc
     6241 ggcccgcttc ttcggccagc tcgcgctgct tgaccagacc ggcggcctgg ttggcgaccg
     6301 cgccgagcac ccggcggtca cgtgcggaga gcttcttgcc ggccagcagc atccagaact
     6361 cgtcgtcgcc gacttcgatc gcggtgtcgg cggtgtcaac cgtcacgcaa ggttcgttgc
     6421 ccacacaggc gactacgtag ggctttccgc cgtt
//
LOCUS       DASSGQ010000381         3604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1457969,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000381 DASSGQ010000000
VERSION     DASSGQ010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457969"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VFI55_16790"
     CDS             <1..737
                     /locus_tag="VFI55_16790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075719.1"
                     /translation="GYRRIHGELAALGIKIAASTVWEILKNAGIDPVPGRDRQTWTTF
                     LRSQAHAILAADFFETRTVNGGRLYVVAVIEHATRRVRFLGATAHPTAGWTTKLARNL
                     VMDFHDASMTVKDLIRDRDSKYTTAFDAVFVDEGIATVKTGIRVPRMNAIMERWVRTC
                     RAELLDRTLIVNQAHLLHEHGEVRSLLQRAPAPPHAPRRGTPTPAPPTDHRTGPTRPT
                     RHRTTRPTRRHPPRIPTRRLSCPDDI"
     gene            1253..3442
                     /locus_tag="VFI55_16795"
     CDS             1253..3442
                     /locus_tag="VFI55_16795"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7075720.1"
                     /translation="MSTAKHAKSALIAALTPMVVLGVAAQPAMAQAPAPPPIKSYVGD
                     PGRLGDPASWRTPEFNRDIGLVSIGAEFAYAAGYAGGGMNIGLVDSGFFAGHVREHGS
                     LDTNYAVGDRYFSVVAQGGETGPTPGFYDPAFNDTHGTHVSGTVGASRDGVGETQPGG
                     PEANMHGVAFNSNVYLGNTHKTDGVFYGQLPPNATPEQTPDNGYIGNVYRAVNAAQTA
                     NGKPVRIVSSSWGSAPPTENYNTYDGLNAAWRYLSTPDGVADANGNTVHWLNGAIDVA
                     RTGTIIGFSAGNAGVANPTPRGAAPYFMPDLEGRWFTTSGINNNIGRTFNPDGSILVP
                     GQQRFNQCGVAKWSCATAPSTAINSTTVVVVDGVPQPRYQAFSGTSMAAPHSAATLAL
                     IMERFPHMTNEQALYTMFTTGRQNSTINDAAGNAVPNPTAGQIVQVPDRRNGWQTPSL
                     REAMKGPGQLLGPFDVNTQGDTDAWSNDISDVAIRARQQEDADEAAAWEATKIAKGWT
                     NGLPANASDADKSDFAIGTRREQARNERVYVGSLTKRGAGTLFLTGNDSWHGTSTVLG
                     GKLSVVGSHASSIDVRGGTLGGSGSVAGSIDVAGGALQPGLGPDEATGVSDVHIAPGN
                     VLTVGGNVSIGRDGRLAVTIRGDRDYTSVQAAGDLVLDGELTLDVRGALTPGTVLTIM
                     NGKSISGNFHAVPEMRVLLAGGYLFRVSYKDNSVTLTVLRAVPSEAN"
BASE COUNT          718 a         1236 c         1125 g          525 t
ORIGIN      
        1 gggggtacag acgcatccac ggcgaactgg ccgccctggg catcaagatc gccgcatcga
       61 cggtctggga aatcctcaag aacgcaggta tcgatcccgt gcccggccgc gaccgccaga
      121 cctggactac gttcctacgc agccaagcac acgcgatcct cgccgccgac ttcttcgaaa
      181 cccgaaccgt gaacggcggc cgcctatacg tcgtcgccgt catcgaacac gccacccgcc
      241 gcgtccgatt cctcggtgcc accgcgcacc ccaccgcggg ctggacgacg aaactcgccc
      301 ggaatctggt catggacttc cacgacgcca gcatgacggt gaaggacctg atccgcgacc
      361 gcgacagcaa gtacaccacc gccttcgacg cggtcttcgt agacgaaggc atcgccaccg
      421 tgaagactgg cattcgcgtg ccgcgcatga acgcaatcat ggagcgctgg gtgcgcacct
      481 gccgagccga actgctcgac cgaactctca tcgtgaacca ggcgcacctg ctgcacgagc
      541 atggcgaagt acgaagcctt ctacaacgag caccggcgcc accgcacgct ccgcgccgcg
      601 gcacccctac gcccgctccc ccgaccgatc accgaaccgg accgactcgg ccgactcgac
      661 atcgaacgac gcgaccgact cggcggcatc ctccacgaat accgacacgc cgcctgagct
      721 gcccggacga catttaggca cgtacaggtc aacagcgggg ctgatcgggg aagataccgg
      781 cagcagcacg actcctgatg atcttctggc ctaggaaccg aaagatccgc agaagtcgtg
      841 ctcgcctgct gtcatacccc atgacacagg cgattcagtc atgtaaccgg acaaatagaa
      901 acaacgacgg ggccgcggag cagtagtagg ctgtcgatgc ttatgtcgcc taccggcggc
      961 caccgagtgt ccgatgtatt acgtcgatac gcgggcatga tgccgatcaa tatcattttc
     1021 ggtaccgcct ggattcccgg catctccggc ctcgcaagcc gaacgaatct cgcccgtccg
     1081 aattagccac ggaatgcatc gtcgcaagca agccgcctcg ctctcctgac gggcatcaca
     1141 aaaattcgca catcaattga aaataaaagc aaatatccgt ctatgttttg accgatacgc
     1201 cagaccgagg ctcaccctat tccgcacgga acgatcttgg aggaggccga aaatgtccac
     1261 cgcgaaacat gcgaagtcgg cgctgatcgc ggcgctgaca cccatggtgg tgctcggcgt
     1321 tgccgcgcag ccggcgatgg cgcaggcgcc cgcgccgcca ccgatcaagt cctacgtggg
     1381 agaccccggc cggctcggcg acccggccag ttggcgtacg cccgaattca accgggatat
     1441 cggcctggtg tcgatcggag cggagttcgc ctacgccgcc gggtacgccg gcggtgggat
     1501 gaacatcggt ctcgtcgact cgggcttctt cgccgggcac gtacgcgagc acggcagcct
     1561 cgacacgaac tacgcggtgg gtgaccgcta cttctcggtg gtagcccaag gtggcgagac
     1621 gggtccgacc ccggggttct acgacccggc gttcaacgac acccacggca cgcacgtcag
     1681 cgggaccgtg ggcgcaagcc gcgacggtgt cggcgagacg cagcctggcg gacccgaggc
     1741 gaacatgcac ggcgtcgcgt tcaacagcaa cgtgtacctg ggcaacaccc acaagacgga
     1801 cggcgtgttc tacggccaac taccgccgaa cgcgaccccc gagcagacgc cggacaacgg
     1861 ctacatcggc aacgtctacc gggccgtgaa cgccgcgcag acggcgaacg gcaagcccgt
     1921 ccggatcgtc agcagcagtt ggggcagcgc gccgcccacg gagaactaca acacctacga
     1981 cggtctgaac gcggcatggc gctacctgtc cacgccggat ggcgtcgccg acgcgaacgg
     2041 caacaccgtg cactggctga acggagccat cgacgtcgcc cgcaccggca cgatcatcgg
     2101 gttcagcgcc ggcaacgccg gcgtggcgaa cccgacgccg cgcggcgccg caccgtactt
     2161 catgccggac ctggagggcc gctggttcac cacgtcgggg atcaacaaca acatcgggcg
     2221 cacgttcaac cccgacggtt cgatcctggt cccgggacag caacgattca accagtgcgg
     2281 tgtcgcgaag tggtcctgcg cgacggcgcc gagcaccgcg atcaacagca ccaccgtggt
     2341 agtcgtcgac ggcgtgccgc agccgcgcta tcaggccttc tcaggaacgt cgatggccgc
     2401 cccgcactcg gcggccacgc tggcgttgat catggagcgc ttcccgcaca tgaccaatga
     2461 gcaggcgctc tacacgatgt tcaccaccgg tcggcagaac agcacgatca acgacgcggc
     2521 gggcaacgcc gttccgaatc ccacggcggg ccagatcgtg caggttcccg accggcgcaa
     2581 cggctggcag acgccaagcc tgcgggaggc catgaagggt ccgggccagc tgctcgggcc
     2641 gttcgacgtc aacacgcagg gagacaccga cgcatggtcg aacgacatct ccgacgtcgc
     2701 gatccgcgca cgccagcagg aagacgccga cgaagcggcc gcctgggagg ccaccaagat
     2761 cgcaaagggt tggacgaacg gcctgcccgc caacgccagc gacgccgaca agtccgactt
     2821 cgccatcggc acccgccgcg agcaagcacg caatgaacgc gtctacgtgg gcagcctcac
     2881 caagcgcggc gcgggcaccc tgttcctcac cggcaatgac agctggcacg gcacgagcac
     2941 cgtcctcggc gggaagctct cggtcgtggg ctcgcacgcg agctcgatcg acgtacgcgg
     3001 cgggacgctc ggcggcagcg gcagcgtcgc cggcagcatc gacgtcgccg gtggcgcgct
     3061 gcagcccggt ctcggacccg acgaagccac gggcgtctcc gatgtgcaca tcgctcccgg
     3121 caatgtcctg accgtcggcg ggaacgtgag catcggcagg gacggtcgcc tagcggtgac
     3181 catcaggggt gaccgcgact acaccagcgt ccaggccgca ggtgatctcg tactggacgg
     3241 ggagctgacc ctggatgtgc gcggagccct gaccccgggc accgtactga cgatcatgaa
     3301 tggcaagtcg atcagcggca atttccatgc ggtgcccgaa atgcgcgtcc tgctcgcggg
     3361 cggctacctg ttccgggtgt cctacaagga caacagcgtg acgctgaccg tcctgcgcgc
     3421 ggtaccgtcc gaggcgaact gagccctgat cgttttgttc gactcggctt gaccgggatc
     3481 gagtcgtggt gaagggacat ggggcctggt ggcagggagg agactgtggg ccgtgacgcc
     3541 cacggccccg tggttgtctc tgtttgtccg gttacatgac ttaatcgcct tgtgttatgg
     3601 agtg
//
LOCUS       DASSGQ010000382        15041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1244444,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000382 DASSGQ010000000
VERSION     DASSGQ010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15041
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244444"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFI55_16800"
     CDS             <1..310
                     /locus_tag="VFI55_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075721.1"
                     /translation="PPRPETAYGEWKAGWVDFDGTSLQIGAARGDPGPFVNGDGAQLA
                     SGDSLSYDDYRCRADQAGVFCVNYAHQSAVRFATAGIQPFGCLRSVPPPDGVGTAFSC
                     "
     gene            complement(338..3016)
                     /locus_tag="VFI55_16805"
     CDS             complement(338..3016)
                     /locus_tag="VFI55_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar epimerase family protein"
                     /protein_id="HET7075722.1"
                     /translation="MRIAVTGASGVLGRGLAGHLLSQGHDVVGLARHRPQSWSNEAEF
                     VAADIRDAGAVKRFVAGADVVAHCAWAKNPGPEDRISQQVNVGGALNVLEAMAETGTG
                     RIVFPSSAQVYGRRTAPQAESDRITPVSADGLHMARVEEMLAASDVESVAIRCALIVG
                     RNVDNWVRRLMAMPAFPDRAIDQPLQLVHLDDALRLLARAVLDAEIDSGAVNLAAPGA
                     PTLRQIAAGLGRPVVWSRGPLAALRRGGSTSHTELELVQSAPLMDTSLLRDGWGFQPA
                     WSADECVEDLALAVRGRVTVGKWVLSLPWRLTNISDLPAADAPAEDGVVPRLAGPEGV
                     NGEFDTPIDPRFPTYLATNLSEALPGPFSPSSASVTVRGLRAGGVGIAERLRPGGMIQ
                     REIAVRTVAVFAHRLYGAITSAHFMAETVPFAKPSTIVGNSGFFGPSMASLPIFGEER
                     LPLRSSRARRRLRTLRNVGVFGVNLVGLSAGSPLDTQAFVADVDRLERLAGGDLAALD
                     DRRLGSLILLARDHVVHGWVLASGSFMLCAAFNVLLRGLCGRDTAPPAGPELVSARSV
                     EAVQRLVAAARREPDVMRLLNQPGKHLDVLAAQAPAFHSAVLAELALIGHRGPAEVEM
                     ASTSYADDPELLVRMVARTINAPPAPQPVHAPIPLHAKPVALLAARQLRDREVRRDKM
                     VRAIWVLRGLLREYGRRLSDAGVFDSADDVFYLLVDELHEIERLPPDAAQLVARRRAE
                     QRRLAGVVPPTVFSGNWLPSTTSSPALSSGETLRGVGVCGGRVRGRVRIVRPETIDDL
                     QPGEILVAEVTDVGYTAAFCYAAAVVTELGGPMSHAAVVAREFGFPCVVDAQGATRSL
                     PPGALVEVDGTTGEIHVLELTPDDSPPLSARERGGS"
     gene            complement(3123..>15041)
                     /locus_tag="VFI55_16810"
     CDS             complement(3123..>15041)
                     /locus_tag="VFI55_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HET7075723.1"
                     /translation="GRCKSYAGAADGVGWSEGGAVLVVERLSDARRLGHPVLAVVRGS
                     AVNQDGASNGLTAPNGPSQQRVVRSALANAGLSAADVDVVEGHGTGTTLGDPIEAQAL
                     LATYGQDRAEPLWLGSIKSNMGHTQAAAGVAGVIKMVQAMRHETLPATLHVDEPSSHV
                     DWTAGAVSLLTEARPWKAESANGRVRRAGVSSFGISGTNAHVIVEAPPTEQRAVASTT
                     PVVPWVISAKSDAALRAQAARLAEHVGAHPELDVADVGWTLAGRSTFEHRAVVVDGDR
                     DRLLAGLHELAADEPVSVIRGTAAPAGKTVFVFPGQGSQWLGMGVELLDTAPVFARQM
                     RECAEAFTEFVDWSLIDVLRGAPDAPGMDRVDVVQPVLFAVMVSLAELWKSVGVRPDA
                     VIGHSQGEIAAAYVAGALSLRDAARVVTLRSKLLRSLARPGGMLSIACSTERARELLA
                     PYGNRVSIAAVNGRSAVVVSGEVAALDELAGFCADLELRTHRIDVDYASHSVEVEAIH
                     GELVRVLAGIDPRSSRTAFFSTVTGSRLDTAGLDPEYWYRNIRQTVQFEQAVRSACEH
                     GYRTFIESSPHPALIAGVEDTFTDCTNGDTEAIVVATLGRDDGGLQRFLTSAATAFVA
                     GVAVDWRGVLTGAGFVELPTYAFDRRRFWLSGEGLGADAAGLGLAAGEHALLGAVVEL
                     PASGGVVLTGRLSPNVQGWLADHAVAGVVVFPGSGFVELAIRAGDEVGCEVVDELTLQ
                     APLIVPAAASGLASVAVQVVVGAAEAPSPAQREVSPLARGGSGRRSVSIFSRADAGAG
                     WVCHAEGTLSSGSVEPSVDLSEWPPVGAVAVSLADGYERLHARGYAYGPAFRGLTAAW
                     VRGDEVFAEVRLPDAAGGVGGFGVHPALLDAAMHAFLMPKVLAGEAAGPDTEVALPFS
                     WQGVSLHAAGASAVRARIAPAGPAGAAAVSIELADGLGLPVLSVGAMVARSVSERQLR
                     AAVSGSGPDRLFQVMWSPATATAPAGGRAPAHQVFESVAAEGDPVTQTYQRTHQALAA
                     VQSWLTGSDSGVLLVVTRGAMVLPGEDVTDLAGAAVWGLVRSAQTEHPGRIVLVDCDT
                     PLDDSAGDTVAAVLAVGEPQVLLRGGTVYTPRVHGSRAADGLLVPPGDGAPWRLGLSS
                     AGTFENLRLEPVPNADAPLQPGQVRVAMRAIAANFRDIMITLGMFTHDALLGGEGAGV
                     VVDVGPGVTEFAVGDSVFGFFPDGSGTLVAGDVRLLLPMPAGWSYAEAAAISAVFTTA
                     YYAFVHLADVKPGQRVLVHAGTGGVGMAAVQLARHLGLEVFATASRGKWDTLRATGFD
                     EDHISDSRSLEFEDKFRAVTGGRGFDVVLDSLAGEFVDASLRLVAPGGVFLEMGKTDI
                     RDPGVIAQEYPGVRYRAFDLFEPGRPRMHQYMLELAELFDAGVLRPLPVTTFDVRRAP
                     AALRYLSQARHVGKVVMTMPTAPGGALGAGTVLITGGTGMAGSALARHVVTRHGVRNL
                     VLLSRSGPDAPGAAELVNELRAAGAQVRVVACDAADREALAKLIADIPERQPLTGVIH
                     AAGVLDDAVVTSLTPERMDVVLRAKVDAAWHLHELTRDLDVSAFVMFSSMAGLVGASG
                     QGNYAAANSFLDGLAVHRRAHGLSAMSLGWGLWDQASAMTRGLDAAGLARLGRDGVLA
                     LSSDEALELFDTALVVDEPFLAPARIDLAALRAHAVAVPPMFTDLVNAPARRQVDDSL
                     AAAKSKSALAHRLHGLPEPEQHAIVLDLVRSHIATVLGNITPDAIDPDKAFQELGFDS
                     LTAVEMRNRLKSATGLSLSPTLIFDYPTPGGLAKYMRAELAGVPQEIKHTPAVRSAGD
                     DPIAIVGMSCRYPGGVNSPDDLWDMLIQGRDVLSEFPNDRGWDLAGLFNPDPDVPGAC
                     YTRTGGFVDGVADFDPAFFGVGPSEALAMDPQQRMFLELSWEALERAGIEPGGLRGSA
                     TGVFAGVMTQGYGMFAAEPVEGFRLTGQLSSVASGRVSYVLGLEGPAVSVDTACSSSL
                     VALHMAVQSLRSGECDLALAGGVTVNATPDIFVEFSRWRGLSPDGRCKAFAGAADGTG
                     FSEGGGMLVVERLSDAQRLGHPVLAVVRGSAVNQDGASNGLTAPNGPSQQRVVRAALA
                     NAGLTATEVAVVEGHGTGTTLGDPIEAQALLATYGQGRTEPLWLGSIKSNMGHTQAAA
                     GVAGVIKMVLAMRHEMLPATLHVDEPSPHVDWTAGSISLLDEARPWPPNGRVHRAGVS
                     SFGISGTNAHVIVEAAPAVPEREAGPSLPVVPWVISAKSAPALRAQAARLAEHVRARS
                     EREIAPEIRDVGATLAGRSMFEHRAVVVGADRDRLLAGLDELVADEPVSVIRGTATPA
                     GKTVFVFPGQGSQLLGMGMGLHAGYPVFAEAFNTVVGELDRHLLRPLREVMWGHDESL
                     LNTTEFAQPALFAIEVALFRLLESWGVKPDFVMGHSVGELSAAHVAGVLSLQNAAVLV
                     AARGRFMQALPAGGAMFAAQATEEEVRPLLNAEVGIAAINGPASVVISGDERAATAIA
                     DQLRADGHRVHRLAVSHAFHSPLMDPMIDEFRTVAAGLAVGQPSLPIVSNVTGQLAGD
                     DFASAAYWQRHIREAVRFADSVRFAHSAGATRFFEVGPGSGLTASIEEVLSGDGQPPV
                     ITMSALRKDRPEPATLTNAVAQGFVAGMDVNWRAALGSADFVELPTYAFERRRFWLSA
                     AGAAADAAGLGLAAGDHALLGAVVELPASGGVVLTGRLSPAVQGWLADHTVGGVVLFP
                     GAGFVELAIRAGDEVGCGVVDELNLAAPLVLPPGGSVAVQVVVDGPDESGTRAVSVFS
                     RADTGSGWLLHAEGALSAGSVEPSADLSVWPPVGAVAVDVGDGYQRLADRGYGYGPAF
                     RGLTSMWRRGDEVFAEVTLPADAGLAVAGFGVHPVMLDAALHAVILASADDELGHGSV
                     LVPFSWQHVSLHAAGASAVRARIVPTGPSSVSIDLADGLGLPVLSVGSMVARPVSDRQ
                     LLAAVSNTGPDRLFEVVWSALPSSAAQPVLVRTWGTTEPETGETEPAAMLFESVPVVG
                     DVAAGVRAAAQSVLEVLRSWLARDGSGMLVVSTRGAVGMPGEDVTDLAGAAVWGLVRS
                     AQTEHPGRIVLVDSDAPWDDSAGAAVAAVLAPGEPQVLLRGGRVYTARVRGSRAVSGV
                     LVPPGDGPWRLGMSSRGTFENLRLERIPDAEAPLQPGQVRVALSAIAANFRDVMIALG
                     LYPDPDAIMGVEASGVISETSPETGAEIASGTGRFAVGDRVMGLFPDGTGTVAVTDER
                     LLVKVPVGWSHTAAATTSVVFATAFYALADLARVKPGQRVLVHAAAGGVGMAAVQLAR
                     HLGLEVFATASRGKWDTLRAMGFDEDHISDSRSLEFEEKFRAVTGGRGFDVVLDSLAG
                     EFVDASLRLVAPGGVFLEMGKTDIRDPGVIARQYPGVRYRAFDLFEAGPDRIRQILDE
                     LAALFGDEVLRPLPVTTFDVRRAPAALRYLSQARHVGKVVMTMPTAPGGALGAGTVLI
                     TGGTGMAGSALARHVVTRHGVRNLVLLSRSGPDAPGAAELVEELGASGARVQVLACDA
                     ADREALAKVIADIPVQRPLSAVIHAAGALDDAVVTSLTPERMDVVLRAKVDAAWNLHE
                     LTRDLDVSAFVMFSSMAGLVGASGQGNYAAANSFLDGLAVHRRAHGLPAISLGWGLWD
                     QASAMTGGLATVDFKRFARDGIVAMSSAEALQLLDTAMIVDEPFMLPAHIDFAALRVK
                     FDGGTLPPMFVDLINAPTRRQVDDSLAAAKSKSALLQRLEGLPEDEQHALLLDLVRSH
                     IATVLGNASPEAIDPDKAFQELGFDSLTAVEMRNRLKSATGLGLSPTLIFDYPNSAAL
                     AGYMHRELVGASEQRAPAAAPGEAEIQRVVGSIPVKRLRQAGVLELLLALANEADGGA
                     LQGHTAATAAAAEKGSEKDIAKDIADMDLDDLVNAALMDDDE"
BASE COUNT         2592 a         5617 c         4818 g         2014 t
ORIGIN      
        1 cccgccgaga cccgagacgg cctacggcga atggaaagcg ggctgggtcg acttcgacgg
       61 aacgagcctg caaatcggag cggcccgcgg cgatccgggt ccgttcgtca acggcgacgg
      121 cgcccagctg gccagcggag actcgttgtc atatgacgac taccgttgcc gcgccgatca
      181 ggcgggcgtg ttctgcgtga actatgccca ccagtcggcg gtacggtttg ccaccgccgg
      241 catccagcct ttcggttgcc tgcggtcggt gccgccgccg gatggcgtcg gtaccgcgtt
      301 cagctgctga acctgcgacg gcttggcata tgcggtttca gctgccgccg cgctctcggg
      361 ccgacagcgg tgggctgtcg tcaggggtca gttcaagcac gtgaatctcg cccgtggtcc
      421 cgtcgacctc gacgagcgcc cccggcggca gggatcgggt ggcgccctga gcgtccacca
      481 cacaagggaa cccgaactcg cgggccacca cggcggcgtg cgacatcgga ccaccgagct
      541 cggtcaccac ggcggcggcg tagcagaacg cggcggtgta gccgacgtcg gtgacttccg
      601 cgacgaggat ctcgccgggt tgcaggtcat cgatggtctc gggccgcaca atccgcaccc
      661 ggccgcggac gcgtcccccg cagacaccga ctccacgcag ggtttccccg ctggacaggg
      721 ccggtgacga cgtggtggac ggcagccaat tcccgctgaa aaccgttggc ggaacgacgc
      781 cggccagcct gcgctgttcg gcccgccgcc gggccaccag ctgcgcggcg tccggcggga
      841 gccgctcgat ttcatggagc tcgtcgacca gcagatagaa cacgtcgtcg gcgctgtcga
      901 acactccggc gtcggacaac cggcgcccgt actcccgcag caggccgcgc agcacccaga
      961 tggcccggac cattttgtcg cggcggacct cacggtcgcg cagttggcgt gccgccagaa
     1021 gcgctacggg tttggcgtgc aacggaatcg gggcatgcac gggctgcggc gcgggaggcg
     1081 cgttgatcgt tctggccacc atgcgaacca gcagttcggg gtcgtcggcg taactggtcg
     1141 atgccatctc gacctccgcc ggaccgcggt gtccgatcaa tgccagctcg gccagcaccg
     1201 cggaatgaaa cgccggtgcc tgcgcggcaa gcacgtcgag gtgcttgccg ggttggttca
     1261 gcaggcgcat cacgtcgggt tcgcgccgcg cggcggccac cagccgctgc accgcctcca
     1321 ccgaccgcgc gctgaccaat tccggtccgg cgggcggggc ggtgtcgcgc ccgcacaagc
     1381 cgcgcaacag cacgttgaac gccgcacaca gcatgaacga ccccgaggcg agcacccagc
     1441 catggacgac gtggtcgcgc gccaacagga tcaggctgcc cagccggcga tcgtcgagtg
     1501 cggcgaggtc gccacccgcg aggcgctcga gacggtcgac atcggcaacg aaggcctggg
     1561 tgtcgagggg ggaaccggcg ctcaggccga ccaggttgac gccgaatacc ccgacgttgc
     1621 gcagggtgcg caatcgcctt cgggcacggc tggatcgcag gggtagacgc tcctcaccga
     1681 agatcggcag ggacgccatg ctgggaccga agaacccgct gttgccgaca atcgtcgacg
     1741 gcttggcgaa cggcacggtt tcggccatga aatgcgccga cgtgatggcg ccgtacagcc
     1801 ggtgggcgaa caccgcgacg gtccgcaccg cgatctcccg ctggatcatc ccgcccggcc
     1861 gcaatcgctc ggcgatgccg acgccaccgg cgcgcaggcc gcgcacggtc accgacgccg
     1921 atgacggcga gaacgggccg ggcagcgcct ccgacagatt ggtggccagg tacgtcggga
     1981 aacgcgggtc gatcggcgta tcgaactcgc cgttgactcc ttctggccca gccaatctgg
     2041 gcaccactcc gtcctcggcg ggtgcgtcgg cggcaggcag atccgagatg ttggtcagcc
     2101 gccagggcag cgatagcacc cacttgccca ccgtgacccg gccgcgcacc gccagcgcga
     2161 ggtcttcgac gcattcgtcg gcgctccacg ccggttgaaa tccccatccg tcgcgaagca
     2221 gcgaggtgtc catcagcggg gcgctctgca ccagttccag ctcggtgtgc gaggtcgacc
     2281 cgcctcgccg cagcgcggcg agcgggccgc gcgaccacac gaccgggcgc cccagtcccg
     2341 cggcaatctg acgcagtgtc ggcgcacccg gggcagcgag attgactgca ccgctgtcga
     2401 tttcggcgtc taggaccgcg cgggccaaca accgaagcgc gtcgtcgagg tgcaccagct
     2461 gcagcggctg gtcgatggct cggtcgggaa acgcgggcat cgccatcagc cggcgcaccc
     2521 aattgtcgac gttgcgaccc acgatcagcg cgcagcggat cgcgaccgat tcgacgtcgg
     2581 aggccgcgag catttcttcg acgcgggcca tatgcaggcc gtcggcagac accggggtta
     2641 tccggtccga ctcggcctgc ggggcagttc gccggccgta gacctgtgcc gacgaaggga
     2701 aaacgatgcg cccggttccg gtctcggcca tcgcctcgag aacgttgagg gcgcctccga
     2761 cgttgacctg ttggctgatt cggtcctcgg ggcccgggtt ctttgcccat gcgcaatggg
     2821 cgacgacgtc cgcgccggcg acgaagcgct tgaccgcgcc cgcgtcccgg atgtccgccg
     2881 cgacgaattc tgcttcattc gaccaacttt ggggtcggtg acgcgcaagc ccgacgacat
     2941 cgtggccttg gctgagcaag tggccggcca gaccgcggcc gagcacaccg ctggccccgg
     3001 tgaccgcgat cctcactgac gcccctcgtt catgcgcgcc ccgttacgtg gcaagcgaac
     3061 tcggaccccc cacccacttg aggggcttgc ccccaacgca tcgccgccgt cgcagctcac
     3121 ggctactcgt cgtcgtccat caacgccgcg ttgaccaggt cgtcgaggtc catgtccgcg
     3181 atgtcttttg cgatgtcttt ttcgctgccc ttttcggcgg ccgcagcggt ggccgccgtg
     3241 tgtccctgca acgcaccgcc gtcggcctcg ttggccaacg ctagcaacag ctccaagact
     3301 ccggcctggc gaagccgctt gaccggaatc gatccgacga cgcgctggat ttcggcttcc
     3361 ccgggtgccg cggccggggc gcgctgctcc gatgcgccga cgagttcgcg gtgcatgtag
     3421 ccggccaggg cggccgagtt cgggtagtcg aagatgagcg tcggtgaaag cccaaggccg
     3481 gtagcggatt tgagccggtt gcgcatctcg actgcggtca gcgagtcaaa gcccaactcc
     3541 tggaacgcct tgtccgggtc gatggcttcg gggctcgcgt tgcccagcac ggtggcgata
     3601 tgggaacgca ccaagtccag cagaagggca tgttgctcgt cctcgggcag cccttcgagg
     3661 cgttgcagca gggccgattt cgatttcgcg gcggccagcg agtcgtccac ctggcgccgc
     3721 gttggcgcgt tgatcaagtc gacgaacatc ggcggcagcg tgccgccgtc gaacttgacc
     3781 cgtaacgccg cgaaatcgat atgggcgggc agcatgaacg gctcgtcgac aatcatcgcg
     3841 gtgtcgagca actgcagggc ttcggcggag gacatcgcca cgatgccgtc ccgggcgaac
     3901 cgcttgaaat cgacagtcgc cagcccgcct gtcatggcgc tggcctgatc ccacagaccc
     3961 cagcccagcg agatcgccgg cagcccatgc gcccgccgat gcacggccag cccgtccagg
     4021 aaggagttgg ccgccgcata gttaccctga cccgacgcac ccaccaaccc ggccatcgac
     4081 gaaaacatca cgaacgccga cacatccaga tcgcgggtca actcgtgcag gttccacgcc
     4141 gcatccacct tggcccgcaa caccacatcc atccgctcgg gcgtcagcga cgtcaccacc
     4201 gcgtcatcca gcgcacccgc cgcatggatc accgccgaca gcggacgctg gaccgggata
     4261 tcggcgatca ccttggccaa cgcctcccga tcggccgcat cgcaggccaa cacctgcacc
     4321 cgggcgcccg aggcgcccaa ttcctccacc agctccgcgg cccccggcgc gtcggggcca
     4381 ctgcgactga gcaacaccag gtttcgcacc ccgtgccggg tcaccacgtg acgggccaac
     4441 gccgaacccg ccatcccggt cccgccggtg atcaacaccg tgcccgcacc cagggcacca
     4501 cctggcgcag taggcatcgt catgacgacc ttgccgacat ggcgggcctg actcagatac
     4561 cgcagcgcgg ccggcgcccg ccgcacgtca aatgtcgtca cgggcaaggg ccttagcacc
     4621 tcgtcgccga acagcgcggc cagctcgtcg aggatctgcc gaatgcgatc cggtccggct
     4681 tcgaagaggt cgaaggcgcg gtagcgcacg ccgggatatt gccgggcgat cacgcccggg
     4741 tcgcggatgt cggtcttgcc catctccaaa aacaccccac cgggtgcgac cagtctcagg
     4801 gaggcgtcga cgaattcccc ggccagcgag tccagcacca cgtcgaagcc gcgaccaccg
     4861 gtgaccgcgc ggaacttctc ctcgaactcc aggctgcgcg aatcggagat gtggtcctcg
     4921 tcaaatccca tggcccgcaa cgtatcccac ttaccgcggc tagccgtggc gaacacctcc
     4981 agccccagat gccgggccaa ctgcaccgcc gccatcccca ccccgccggc ggcggcgtgc
     5041 accaacaccc gctggccggg cttgacacgg gccaggtctg ccagcgcgta gaaggcggtg
     5101 gcgaaaacca ccgacgtggt ggcggccgcg gtgtgcgacc acccgaccgg caccttgacc
     5161 agcagccgct catcggtcac ggcgacggtg ccggtgccgt cggggaacag ccccatcacc
     5221 cggtcaccga cggcgaaacg gccagttccc gaggcgattt cagccccagt ttcagggctg
     5281 gtttctgaga tcacgccgga ggcctcgacg cccatgatcg catcggggtc gggatagaga
     5341 cccaaggcga tcatgacatc acggaagttg gccgcgatgg ccgacaacgc cacccgaacc
     5401 tgcccgggct gcagcggcgc ttcggcgtcg gggatgcgct ccagccgcaa attttcgaag
     5461 gtcccgcggc tgctcatgcc caaccgccac ggtccgtctc ccggcggtac caggacacca
     5521 ctgaccgcac ggctgccccg cacccgggcc gtgtaaaccc gtccgccgcg caacaacacc
     5581 tgcggctcgc cgggcgccaa taccgcggcc accgccgcac cggctgaatc gtcccagggc
     5641 gcatcgctgt cgacgagcac gatccggccg ggatgctcgg tctgcgccga ccgcaccaac
     5701 ccccacaccg cggcgcccgc caaatccgtg acgtcctccc ccggcatccc caccgcgccc
     5761 cgggtcgaca ccaccagcat ccccgacccg tcgcgcgcca accaggaccg caacacttcc
     5821 agcaccgatt gtgcggccgc ccgcacgccg gcggcgacgt ccccgaccac tggcacggac
     5881 tcaaacagca tcgccgcggg ctcagtctcg ccggtttcgg gttccgttgt gccccaagtg
     5941 cgtaccaaca ccggttgcgc cgcggacgat ggcagtgccg accagaccac ctcgaagagc
     6001 cggtccggac cggtgttgga caccgcggcc agcagctgcc ggtcgctcac cggccgcgcc
     6061 accatcgacc ccaccgacag caccggcagc cccaacccat cggccaagtc gatcgacacc
     6121 gaggaagggc ccgtcggcac gattcgcgcg cgtaccgccg acgcaccggc ggcgtgcaac
     6181 gacacgtgct gccacgagaa cggcaccagc accgaaccgt ggccaagctc atcgtcagcg
     6241 gacgccagga tcaccgcgtg caacgccgca tccagcatca ccgggtgcac gccgaacccg
     6301 gccaccgcca acccggcatc ggcgggcagc gtcacctcgg cgaacacctc atcgccgcga
     6361 cgccacatcg acgtcaggcc ccggaacgcc ggcccgtaac cgtacccacg atcggccaac
     6421 cgctggtacc cgtcgccgac atccaccgcg acggcaccca ccggcggcca caccgacaga
     6481 tcggcgctcg gctcgaccga ccccgcgctc agcgcgccct cggcgtgcag cagccagcct
     6541 gaaccggtgt cggcacgcga gaacaccgaa accgcacggg tgccagactc atccgggccg
     6601 tcgacgacga cctgcaccgc caccgaaccc ccgggcggca acaccagcgg ggccgccaaa
     6661 ttcaactcgt cgaccacgcc gcagcccacc tcgtcgccgg cccggatggc caactcgacg
     6721 aaccccgcgc cggggaacag caccacgcca ccgacggtgt ggtcggccaa ccagccctga
     6781 acggcgggcg acaaccggcc ggtcaacacc accccgcccg aggcgggcag ctccaccacc
     6841 gcgcccagca gcgcgtggtc gccggccgcg agtcccaagc cggcggcgtc ggctgccgca
     6901 ccggcagcgg acagccaaaa ccgccgtcgc tcaaaggcat aggtgggtaa ttccacgaag
     6961 tcggcgctgc cgagtgcggc gcgccagttc acgtccatcc cggcgacaaa cccttgcgca
     7021 acggcgttgg tcagggttgc cggctcgggg cgatccttgc gcagcgcgga catcgtgatc
     7081 acgggcggtt gcccgtcgcc ggagagcact tcttcgatgg aggccgtcag gccgctgccg
     7141 ggcccgactt cgaagaaacg ggtggcaccg gcggagtgcg cgaagcggac gctgtcggcg
     7201 aagcgcaccg cttcgcgaat gtgccgctgc cagtacgccg ccgacgcaaa atcgtccccg
     7261 gccagctggc cggtcacgtt cgacacgatc ggcaggctgg gctgcccgac ggcgagcccg
     7321 gcagcgaccg tcctgaattc gtcgatcatc ggatccatca acggcgagtg gaacgcgtgg
     7381 gacacggcca gtcggtggac ccggtggccg tcggcgcgga gttggtcggc gattgccgtg
     7441 gccgcgcgtt cgtcacccga aatcaccacc gaggcaggac cgttgatcgc cgcgatgccg
     7501 acctcggcgt tcagcagcgg ccgcacttcc tcctcggtgg cctgtgcggc gaacatcgcc
     7561 ccgccagcag gcagcgcctg catgaaccgg ccacgggccg cgaccagcac cgcggcattc
     7621 tgaagcgaca gcaccccggc gacgtgggcc gccgacagct cgccgaccga gtggcccatc
     7681 acgaaatcgg gcttgacgcc ccaggattcc agcagccgga acagggcgac ttctatcgca
     7741 aacagcgccg gctgcgcgaa ttcggtggtg ttcaacaggc tttcgtcgtg gccccacatc
     7801 acttcgcgta atggccggag caggtgccgg tccagctcgc ccaccacggt gttgaatgcc
     7861 tcggcgaaca ccgggtatcc ggcgtgcaat cccattccca tgccgagcag ttgggagcct
     7921 tggccgggga acacgaagac ggtcttgccc gcgggtgtgg cggtgccccg aatgaccgat
     7981 acgggctcat cggcgaccag ctcgtccagc ccggccagca accggtcccg gtcggcaccc
     8041 accacgacgg cccgatgctc gaacatcgat cggcccgcca acgtcgcgcc cacatcgcga
     8101 atttcagggg cgatttcacg ctcgctccga gcccgcacat gctcggccag ccgcgccgcc
     8161 tgagctctca acgccggagc cgatttcgcc gagatcaccc acggcaccac cggcaggctc
     8221 ggtcccgcct ctcgctccgg aaccgcgggg gcggcctcca cgatcacgtg cgcattggtg
     8281 ccgctgatac cgaacgacga cacgcccgcg cggtgcaccc ggccgttcgg cggccagggc
     8341 cgcgcctcgt ccaacaggga tatcgagccc gccgtccagt caacgtgggg gctgggttcg
     8401 tcgacatgca gggtggccgg caacatctcg tggcgcatgg ccaacaccat cttgatcaca
     8461 ccggccacgc ccgcggcggc ctgcgtgtga cccatgttcg acttgatcga gcccagccag
     8521 agcggctcgg tgcggccttg tccgtacgtc gccaacaacg cctgggcctc aatgggatcg
     8581 cccaacgtgg tgccggtgcc gtggccttcg accacggcca cttcggttgc cgtcaacccg
     8641 gcgttggcca gcgccgcccg caccacccgc tgctgtgatg ggccattggg cgcggtcaac
     8701 ccgttcgacg caccgtcctg gttgaccgcc gagccccgca ccactgccag caccggatgg
     8761 cctaaccgct gcgcatccga cagccgctcc accaccagca taccgccgcc ctcggagaat
     8821 ccggtgccgt cggcggcccc ggcaaacgcc ttgcagcgtc cgtccggtga gagtccgcgc
     8881 cagcggctga attccacgaa gatgtcgggt gtggcgttga cggtgacacc gccggccagc
     8941 gccaggtcgc actcgcccga gcgcagcgac tgcaccgcca tgtgcaacgc caccaacgac
     9001 gacgaacacg ccgtatccac cgacaccgcc gggccctcca gccccagcac atacgacacc
     9061 cgccccgagg ccacgctgga cagttgcccg gtcagccgaa agccttccac cggctcggcg
     9121 gcgaacatcc cgtagccctg cgtcattacg ccggcgaaca cgccagtcgc gctgccgcgc
     9181 aggccaccgg gttcaatccc ggcccgctcc aaggcctccc aggacagttc gaggaacatg
     9241 cgctgctggg gatccatcgc cagcgcctcg ctgggcccca ccccgaagaa ggcgggatcg
     9301 aagtcggcga cgccgtccac gaagccgccg gtgcgcgtgt agcaggcgcc gggaacatcg
     9361 ggatcgggat tgaacagtcc ggccaggtcc cagccgcggt cgttcgggaa ctccgacaac
     9421 acgtcgcggc cctggatcag catgtcccac aggtcgtcgg gggaattcac cccgcccggg
     9481 tagcggcacg acataccgac gattgcgatc gggtcgtcac cggcggagcg aaccgcgggc
     9541 gtgtgcttga tttcttgtgg gacaccggcg agttcggctc gcatgtactt ggccaggccg
     9601 ccgggtgtcg ggtagtcgaa gatgagggtg ggtgaaagcg acaggccggt ggcggatttg
     9661 agccggttgc gcatctcgac agcggtcagc gagtcaaagc ccaactcctg gaacgccttg
     9721 tccgggtcga tggcgtcggg ggtgatgttg cccagcacgg tggcgatgtg ggaacgcacc
     9781 aggtccagca cgatcgcgtg ctgttcgggc tcgggcagcc cgtgcaggcg atgcgcgaga
     9841 gccgatttcg atttggcggc ggccagcgag tcgtcgacct gacgacgggc gggcgcgttg
     9901 acaagatcgg tgaacatggg cggcaccgcg acggcgtgtg cgcgcagcgc ggccaaatcg
     9961 atgcgggcgg gcgccaggaa tggttcgtcg acaaccagtg cggtgtcgaa caattcgagc
    10021 gcctcgtccg acgatagggc caacaccccg tcgcggccga ggcgggccag gccggcggcg
    10081 tccagtccgc gggtcatggc gctggcctga tcccacagac cccagcccag cgacatcgcc
    10141 gacagcccgt gcgcccgccg atgcacagcg agcccgtcca ggaaggagtt ggccgccgca
    10201 tagttaccct gacccgacgc acccaccaac ccggccatcg acgaaaacat cacgaacgcc
    10261 gacacatcca gatcgcgagt caactcgtgc aggtgccacg ccgcatccac cttggcccgc
    10321 aataccacat ccatccgctc gggcgtcagc gacgtcacca ccgcgtcgtc gagcacaccg
    10381 gccgcgtgaa tcaccccggt cagcggttgc cgctccggga tatcggcgat cagcttggcc
    10441 aacgcctccc gatcggccgc atcgcacgcc accacgcgca cctgagcgcc ggcggctcgc
    10501 aactcgttga ccagctccgc ggcccccggc gcgtcggggc cactgcgact gagcaacacc
    10561 aggtttcgca ccccgtgccg ggtcaccaca tgacgggcga gcgccgaacc cgccatcccg
    10621 gtcccgccgg tgatcaacac cgtgccggca cccagggcac cacctggcgc agtaggcatc
    10681 gtcatgacga ccttgccgac atggcgcgcc tgactcaaat accgcaatgc cgccggcgcg
    10741 cgccgcacgt cgaacgtcgt gaccggcaag ggccgcagca ccccggcgtc aaacagctcg
    10801 gcgagctcga gcatgtactg gtgcatgcgg ggacggcccg gttcgaaaag gtcgaaggcg
    10861 cgatagcgca caccgggata ttcctgagcg atcacacccg ggtcgcggat gtcggtcttg
    10921 cccatctcca agaacacccc accgggtgcg accagtcgca gtgaggcgtc gacgaattcg
    10981 ccggccagcg agtccagcac cacgtcgaag ccgcgaccac cggtgaccgc gcggaacttg
    11041 tcctcgaact ccaggctgcg cgagtccgag atgtggtcct cgtcaaatcc cgtggcccgc
    11101 aacgtgtccc acttaccgcg gctggccgtg gcgaacacct ccagccccag atgccgggcc
    11161 aactgcaccg ccgccatccc caccccgccg gtgccggcat gcaccaacac ccgctgcccc
    11221 ggcttcacgt cggccaagtg cacgaaggcg tagtacgcgg tggtgaaaac ggctgagatg
    11281 gcggcggctt cggcgtagga ccagccggct ggcatcggaa gcagcagccg cacgtctccg
    11341 gcgaccaggg tgccgctgcc gtccgggaag aatccgaaga ccgaatcgcc gacggcgaat
    11401 tcggtgaccc cgggccccac gtcgaccacc acgccggcgc cttcgccacc gagcaacgcg
    11461 tcgtgggtga acatgcccag ggtgatcatg atgtcgcgga agttggcggc gatggcacgc
    11521 atggcgaccc gaacctggcc gggctgcagg ggcgcgtcgg cattgggaac cggctccagg
    11581 cgcagattct cgaaggtgcc cgcactgctc aggcccaggc gccacggtgc cccatcgccc
    11641 ggcggcacca acaggccgtc agccgcgcgg ctgccgtgca cccgcggtgt gtagaccgtc
    11701 ccaccgcgca gcaacacctg cggctcgccc accgccaaga ccgcggctac ggtgtccccg
    11761 gctgaatcgt caaggggcgt atcgcaatcg accagcacga tccggccggg atgctcggtc
    11821 tgtgccgacc gcaccaaccc ccacacggcc gcaccggcca aatcggtgac gtcctccccc
    11881 ggcaacacca tcgcgccccg ggtgaccacc agcagcactc ccgagtcact cccggtcagc
    11941 cacgactgga ccgcggcgag cgcctggtgg gtgcgctggt aggtctgggt taccggatcc
    12001 ccttcggcgg caacggattc gaacacttgg tgggccgggg cgcggccccc ggccggcgcg
    12061 gttgccgtcg ccggtgacca catcacctgg aacagccggt cggggcccga gcccgacacc
    12121 gccgcccgca gctgccgttc gctgacggag cgggccacca tcgcgcccac cgacaacacc
    12181 ggcaatccca acccgtcggc cagctcgatc gacaccgctg cagccccggc cggaccggcc
    12241 ggcgcgatcc gcgcccgcac ggccgaggcg cccgcggcgt gcaacgacac gccctgccag
    12301 gagaacggca acgccacctc ggtgtccggg ccggcggcct cgccggccaa caccttgggc
    12361 atcaagaagg cgtgcatcgc cgcgtccagc agcgccgggt gcacaccgaa gccgccgacc
    12421 ccgccggccg cgtccggcaa ccgcacctcg gcgaacacct cgtcgccgcg tacccacgcg
    12481 gcggtcaatc ccctgaacgc cggaccgtag gcgtagccgc gggcgtgcag tcgctcatag
    12541 ccgtcggcca ggctgacggc gaccgcaccc accggcggcc actccgacag atccacgctg
    12601 ggctcgacgc tcccggagct caacgtcccc tcggcgtggc acacccaccc ggcgccggcg
    12661 tcggcgcggg agaagatcga cacgctgcgg cgcccggatc ccccgcgagc gagcggggat
    12721 acctcccttt gggcgggtga cggcgcttcg gcggcgccca cgaccacctg caccgcaacc
    12781 gatgccaacc cggaagccgc ggcgggcact atcagcggcg cctgcagcgt cagttcgtcg
    12841 accacctcgc agccgacttc gtcgccggcg cggatcgcca actccacgaa gcccgaaccc
    12901 gggaacacca ccacgccggc gacagcgtga tctgccagcc atccttgaac gttgggcgac
    12961 aaccggccgg tcaacaccac accacccgag gcgggtagct ccaccaccgc gccgagcaac
    13021 gcgtgctcgc cggcggccag ccccaagccg gcggcgtcgg ctccgagacc ttcgccggag
    13081 agccagaatc gccgccggtc aaaggcatag gtgggcagct ccacgaagcc cgcgccggtc
    13141 aggacgccgc gccagtccac ggcaacgccc gcgacgaacg cggtggcggc ggaggtcagg
    13201 aaccgctgca ggccgccgtc gtcgcggccc agggtggcaa cgacgatggc ctcggtgtcg
    13261 ccgttggtgc agtcggtgaa ggtgtcttcg acaccggcga tcaacgcggg atgaggactg
    13321 gattcgatga atgtgcggta gccatgttca cacgcgctgc gcaccgcctg ttcgaactgc
    13381 accgtctgac ggatgttgcg gtaccagtat tcgggatcta aacccgctgt gtccaaacga
    13441 cttccggtca ccgtggagaa gaacgccgta cgcgaggacc gcggatcgat gccggccagc
    13501 acccggacca gttcaccgtg aatcgcctcg acctcaaccg aatgggaggc atagtcgacg
    13561 tcgatccggt gggttcgcag ctccaggtcg gcacagaagc cggccagttc gtccagagcg
    13621 gccacctcac cggacaccac cacggccgac cggccgttga cggcggcgat gctgactcga
    13681 ttgccgtagg gcgccaacaa ctcccgggcc cgttcggtgc tgcaggcgat ggacaacatg
    13741 cccccgggcc gcgccagcga cctcagcagc ttgctgcgca gggtgaccac ccgcgcggcg
    13801 tcgcgcagcg acagcgcgcc cgcgacgtag gccgcggcga tttcgccttg cgagtgaccg
    13861 atcacggcgt ctggacgcac ccccaccgac ttccacagtt cggccaggga caccatcacc
    13921 gcaaacagca ccggctgcac cacgtccacc cggtccatgc ccggagcatc cggcgcgcca
    13981 cgcagcacgt cgatcagcga ccagtccacg aactccgtga atgcctccgc gcactcccgc
    14041 atctgtcgcg cgaataccgg tgcggtatcc agcaattcga cacccatgcc cagccattga
    14101 gagccttggc cggggaaaac gaagacggtc ttgcccgcgg gggcagcggt gccccgaatg
    14161 accgatacgg gctcatcggc ggccagctcg tgcagcccgg ccagcaaccg gtcccgatcg
    14221 ccgtcgacga cgacagcccg atgctcaaag gtcgaccggc ccgccaacgt ccacccgacg
    14281 tcggcaacgt caagctctgg atgcgcgccc acatgctcgg ccaaccgagc ggcctgggcc
    14341 cgcaacgcgg cgtcggattt cgccgaaatc acccacggca ccaccggcgt cgtggaggcg
    14401 acggcacgct gttcggttgg aggtgcctcg acaatcacat gcgcgttggt gccgctgatc
    14461 ccgaacgacg acacgcccgc gcggcgcacc cggccgttcg ccgactcagc cttccaaggc
    14521 cgggcttccg tcagcagtga caccgccccc gccgtccaat ccacgtgcga gctgggctca
    14581 tcgacatgca atgtcgccgg caacgtctcg tggcgcatcg cctgcaccat cttgatcaca
    14641 ccggcgacac cggcggcagc ctgcgtgtga cccatgttcg atttgatcga ccccagccac
    14701 agcggctcgg cccgatcctg gccgtaggtc gccagtagcg cctgggcctc gatcggatca
    14761 cccagggtcg tcccggtgcc gtggccctcc accacatcca cgtcggcggc cgagagcccg
    14821 gcgttggcca gcgcggagcg caccacccgc tgctgcgacg gaccgttggg cgccgtcaac
    14881 ccgttcgacg cgccgtcctg attgaccgcc gaaccccgca ccaccgccaa caccggatga
    14941 cccaaccgcc gcgcgtccga caaccgctcg acgaccagca ccgccccgcc ctcggaccag
    15001 ccgaccccgt cggccgcacc ggcgtacgac ttgcatcggc c
//
LOCUS       DASSGQ010000383         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1123168,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000383 DASSGQ010000000
VERSION     DASSGQ010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123168"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /gene="coaA"
                     /locus_tag="VFI55_16815"
     CDS             complement(<1..687)
                     /gene="coaA"
                     /locus_tag="VFI55_16815"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HET7075724.1"
                     /translation="MPRPSEPSPYVEFDRKRWRALRMSTPLALTEEELFGLRGLGEQI
                     DLLEVEEVYLPLARLIHLQVAARQRLFAATAEFIGEPPQNSDRPVPFVIGVAGSVAVG
                     KSTTARVLQALLARWDHHPRVDLVTTDGFLYSNTELFQRNIIHRKGFPESYNRRALMR
                     FVTSVKSGAEYACAPVYSHLKYDIIPGAKYVVRRPDILILEGLNVLQTGPTLMVSDLF
                     DFSLYVDARIE"
     gene            815..2095
                     /gene="glyA"
                     /locus_tag="VFI55_16820"
     CDS             815..2095
                     /gene="glyA"
                     /locus_tag="VFI55_16820"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177787.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET7075725.1"
                     /translation="MSAPLDEVDPDIAELLGKELNRQRDTLEMIASENFVPRSVLQAQ
                     GSVLTNKYAEGLPGRRYYGGCEYVDVVENIARDRAKALFGSDFANVQPHSGAQANAAV
                     LQALMSPGERLLGLDLANGGHLTHGMRLNFSGKLYENAFYGVDPTTHLIDMDAVRAQA
                     LEFRPKVIIAGWSAYPRILDFPAFRSIADEVDAKLWVDMAHFAGLVAVGLHPSPVPHA
                     DVVSTTIHKTLGGGRSGMILGKQEYAKAINSAVFPGQQGGPLMHVIAGKAVALKIAGT
                     PEFADRQRRTLSGARILADRLLASDVAKAGVSVVSGGTDVHLVLVDLRNSPLDGQAAE
                     DLLHEVGITVNRNAVPNDPRPPMVTSGLRIGTPALATRGFGDAEFTEVADVIAAALVA
                     AGSADVAGLRQRVTRLARELPLYDGLEDWSLVDR"
     gene            2165..2992
                     /locus_tag="VFI55_16825"
     CDS             2165..2992
                     /locus_tag="VFI55_16825"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HET7075726.1"
                     /translation="MAQKPVANALTQELEPVVEANMDRHLNTDDIWFAHDYVPFERGE
                     NFAFLGGRDWDPSQMTLPRVFTDACEILLLLKDNLSAHHRELVDHFILEDWWGRWLGR
                     WTAEEHLHAIALREYLVVTREVDPVANEDARVQYVMKGYRAETYTQVETLVYMAFSER
                     SHAVFCRNLAARLEEPILAGLVDRIARDETRHEEFFSNLVAHCLEYTRDETIAAIAAR
                     AADLQVVGADIDAYQDKVQNVADAGIFGPAELRQAISDRITEWGVADEPALKQFVIA"
BASE COUNT          519 a          999 c          988 g          488 t
ORIGIN      
        1 ttcgatccgg gcatccacat acagcgagaa gtcgaacagg tccgacacca tcagggtcgg
       61 acccgtctgc aaaacgttga ggccctcgag gatcaagatg tccggacggc ggaccacgta
      121 tttggcgccg ggaatgatgt cgtatttcag gtgtgaatac accggcgcgc acgcgtactc
      181 ggcaccggat ttgaccgagg tgacgaaccg catcagcgcg cgacggttgt agctctctgg
      241 aaaacccttg cggtggatga tgtttcgctg aaacagctcc gtgttcgagt acagaaagcc
      301 gtcggtggtc accaggtcca cccgggggtg gtgatcccac cgggccagca gcgcctgcag
      361 cacgcgcgcg gtggtcgact tgcccaccgc cacgctgccg gccacgccga tgacgaacgg
      421 caccggccgg tccgagttct gcggaggttc gccgatgaat tccgcggtag cggcgaacag
      481 ccgctggcgc gcggcgacct gaaggtgaat cagacgcgcc agcggtaggt aaacctcttc
      541 gacttccagc aggtcgatct gctcaccgag tccgcgcagg ccgaacaatt cttcttcggt
      601 gagggccagc ggcgtcgaca tgcgaagcgc gcgccatcgc ttcctgtcga actccacata
      661 gggactcggc tcgcttggcc gcggcatagt gccagtgttg caggggcgga cgtgcgcccg
      721 acgcctgggc tggctaaaag cgccgctgga tagactggcg tccgtgaccg ctgcacctga
      781 cgcccgaacc gctgcctccg acaccgcatc ggtgatgtcc gctccgcttg acgaggtcga
      841 ccccgatatc gccgagttgc tgggcaagga gctcaaccgg caacgcgaca ccctggagat
      901 gatcgcgtcg gagaacttcg tgccgcgctc ggtgctgcag gcacagggca gcgtgctgac
      961 caacaagtac gccgaggggc tgcccggccg gcgctactac ggcgggtgcg agtacgtgga
     1021 cgtggtggag aacatcgccc gcgaccgcgc caaggcgttg ttcggctccg acttcgccaa
     1081 cgtccaaccg cattcgggcg cgcaggccaa cgccgccgtc ctgcaagccc tgatgtcgcc
     1141 gggggagcgg ctgctgggtc tggatctcgc caacggcggc cacctgaccc acggcatgcg
     1201 gctgaacttc tcgggcaagt tgtatgaaaa cgccttctac ggcgtcgatc cgacgacgca
     1261 cctgatcgac atggacgcgg tgcgcgccca ggcgctcgaa tttcgtccga aggtgatcat
     1321 cgccgggtgg tcggcgtacc cgcgcattct ggacttcccg gcgttccggt cgatcgccga
     1381 cgaggtggac gccaagctgt gggttgacat ggcgcatttc gcgggcctgg tcgccgttgg
     1441 gctgcacccc tccccggtgc cgcacgccga tgtggtctcc acgaccatcc acaagacgct
     1501 cggcggaggg cgttccggaa tgatcctggg caagcaggag tacgccaagg ccatcaactc
     1561 ggcggtgttt cccggccagc agggcggacc gctcatgcat gtcatcgccg gcaaggccgt
     1621 ggcgctgaag atcgccggga cgccggagtt cgccgaccgc cagcggcgca cactgtccgg
     1681 tgcccggatc ctggccgacc ggctgttggc gtccgacgtc gccaaagccg gtgtgtcggt
     1741 ggtcagcggc ggcaccgacg tccacctggt gctggtcgac ctgcgtaact ctccgctgga
     1801 cggccaggcc gccgaggact tgctgcatga ggtcggcatc acggtcaacc gcaatgccgt
     1861 ccccaacgac ccgcggccgc cgatggtgac ctcgggcctg cggatcggca cccccgcgct
     1921 ggccacccgc ggcttcggcg acgcggagtt caccgaggtc gccgacgtca tcgccgcggc
     1981 gctggtggcc gctggttcgg ctgatgtcgc ggggctgcgg cagcgggtga cgcggctggc
     2041 cagggagctc ccgctgtacg acggactcga ggactggtcg ctggtcgacc ggtaatacga
     2101 caagtggccg cgacacggcg cgacctattt tttggaacct gtgtatggag gttacagtta
     2161 ccgcatggca cagaaacctg tcgctaacgc gctgacccag gaactcgaac cggtcgtcga
     2221 agcgaacatg gaccgacacc tcaacaccga cgacatctgg ttcgcccacg attacgtgcc
     2281 attcgagcgc ggcgagaact tcgccttcct cggcggccgc gattgggacc cgtcccagat
     2341 gacgctgccg agggtcttca ccgacgcctg cgagatcctg ctgctgctca aggacaacct
     2401 gtcggcacac caccgcgagc tcgtcgacca cttcatcctc gaggactggt gggggcgctg
     2461 gctgggccgg tggaccgcgg aggagcacct gcacgccatc gcgctgcggg agtacctcgt
     2521 ggtgacccgg gaggtggacc ccgtcgccaa cgaagatgcc cgagtccagt acgtgatgaa
     2581 gggctaccgc gccgagacct acacgcaggt cgaaaccctg gtctacatgg cgttcagcga
     2641 gcgcagccac gccgtcttct gccgcaatct ggccgccagg ctcgaggagc cgatcctggc
     2701 cgggctcgtc gaccggatcg cccgcgatga gacgcgtcac gaggagttct tctcgaatct
     2761 cgtcgcacac tgcctcgagt acacccgcga cgagacgatc gcggcgatcg ccgcgcgcgc
     2821 ggccgacctc caagtcgtcg gcgccgacat cgacgcttac caggacaagg tgcaaaacgt
     2881 tgccgacgcg ggcattttcg gccccgcgga gctgcggcag gcgatctctg accgcatcac
     2941 cgaatggggt gtggccgacg agcccgccct caagcaattc gtcatcgcct agcc
//
LOCUS       DASSGQ010000384         7783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_241377,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000384 DASSGQ010000000
VERSION     DASSGQ010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7783
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241377"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="VFI55_16830"
     CDS             complement(<1..139)
                     /locus_tag="VFI55_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019250109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7075727.1"
                     /translation="MRRPVGTPRPLLRAAVEFINAANGLRPLARKGYATVVVFWFGWP
                     TS"
     gene            complement(139..243)
                     /locus_tag="VFI55_16835"
                     /pseudo
     CDS             complement(139..243)
                     /locus_tag="VFI55_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(308..1123)
                     /locus_tag="VFI55_16840"
     CDS             complement(308..1123)
                     /locus_tag="VFI55_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET7075728.1"
                     /translation="MTDSDSPDTGDGDIDKFDPGLTRRLMALMRPFVKTYFRSEVRGL
                     DSFPPGGALVVGNHSGGMFPMDVPIFSVDFYDKFGYDRPVFTLSHDILFMGPTGQLFR
                     RTGYIRANRENAATALRSGGVVVVFPGGDYDAYRPTLSENVIDFNGRTGYVSTAIDAG
                     VPIVPAVSIGGQETQLYLSRGTWLAERMGLKRLLRSDILPLSFGFPFGFSAAIPPNLP
                     LPTKIVTQVLDPIDITAQFGEHPCVDDVDEHVRSVMQQALKELAAQRRFPILG"
     gene            1582..1923
                     /locus_tag="VFI55_16845"
     CDS             1582..1923
                     /locus_tag="VFI55_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075729.1"
                     /translation="MASLILNRPAARVECSVKAADTADYRQAEYFLGLLAQNRGHIDH
                     RIGDYRKAIAAAEAVGDSEAAIGLRRMARIEEQDRHTVDVLIANLRRRFPLAVPGEVP
                     PVPRRARLAVR"
     gene            2068..3828
                     /locus_tag="VFI55_16850"
     CDS             2068..3828
                     /locus_tag="VFI55_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HET7075730.1"
                     /translation="MGFMSPELPDVDPDTWQARPRAARLQITTRHWVEHGFGTPYAVY
                     LLYLIKIGVYVAAPAAIISLTPGLGGLGRIGDWWTQPIVYQKFVIFTLLFEVLGFGCG
                     SGPLTGRFMPPVGGFLYWLRPNTIRLPPWPGKVPLTGGDTRTVVDVALYAVVLIGGAW
                     ALLSPGQGGPVTATGDVGLIDPVLVAPPIVALAILGLRDKTIFLAARGEHYWLKLIVF
                     FFPFTDQIAAFKLIMLGLWWGAATSKLNHHFPYVVSVMTSNNALLRSRLFNPLKRMLY
                     VDPVNDLRPSWIPKVMAHVGGTTAEFLVPGTLVLIADGQPWRWFLIGFMVIFHLNILS
                     NLPMGVPLEWNVFFIFSLFYLFGHYGAIHALDLRSPLLLAILIVALVIVPITGNLFPQ
                     KISFLPAMRYYAGNWATSVWCFRAGAEDKIEANIVKSSALVINQLAKLYDADTAEIMS
                     DKTAAFRAMHTHGRALNGLLPRAVGGEANEADYSVREGEIVAGPLVGWNFGEGHLHNE
                     QLLEAVQRRCHFEEGDVRVIVLEGQPIHIQRQWYRILDAKTGLIEAGWVDVADMLTRQ
                     PWPEPGDAFPVHVTRQHVTR"
     gene            3979..4794
                     /locus_tag="VFI55_16855"
     CDS             3979..4794
                     /locus_tag="VFI55_16855"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET7075731.1"
                     /translation="MRAVVRCVSAVIGVAATVVPGPPGVSLAAANQSHGFAVASVLPT
                     RGEVVGVAHPVMVTFVAPVDNLAERHAAERAIELKSAPPMTGKFEWLDNREVQWVPDR
                     FWPAHSTVALSVDGESTEFKTGAAVVGVASISEHTFTVTIDGVEATPPPPLPAPHHRP
                     HWGEEGVMPASMGRPEFATPVGSYTVLSKERSVIMDSSSVGIPVDDPDGYRIPVDYAV
                     RITSRGLFVHAAPWAVNSLGFDNVSHGCISLSPEDAEWYYNTVNIGDPVIVQE"
     gene            complement(5021..5878)
                     /locus_tag="VFI55_16860"
     CDS             complement(5021..5878)
                     /locus_tag="VFI55_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020724965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-xylosidase"
                     /protein_id="HET7075732.1"
                     /translation="MTVSDRRNIKVVALAGLCGAAIALSPDAAAAPLKTGGYACVQGM
                     AGDTAALAAAGGAAGAGGAAAACRCATAPATAGGPAAAGAQAATAGAAGAGGPAAAGG
                     PAPAGGPAAAGGPTPAGAPVTVGDVCTASAPLTDMAGVPLVAPGPVPVVPVGAPLIAL
                     GPPVPAAAPVPVGAPVPVGAPIPVGAPVPVGAPVPIGAPVPIGAPVPIGAPVPIGAPV
                     PIGAPVPIGAAVPVGAPVLAGVPVGAPLPIGAPLIDMSGSYRGKGDPIGPAPTGGPVA
                     GQPIAPGPS"
     gene            6353..7384
                     /gene="gap"
                     /locus_tag="VFI55_16865"
     CDS             6353..7384
                     /gene="gap"
                     /locus_tag="VFI55_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET7075733.1"
                     /translation="MTVRVGINGFGRIGRNFYRALLAQQAQGLGSSGADIEVVAVNDI
                     TDNATLAHLLRFDSILGRLPYDVSLEGDDTIVVGTAKIKALEVREGPAATPWADLGVD
                     VVVESTGLFTDAAKAKGHLEAGAKKVIISAPATNPDITIVLGVNDDKYDGSQNIISNA
                     SCTTNCLAPLAKVLHDEFGIVKGLMTTIHAYTQDQNLQDGPHKDLRRARAAALNIVPT
                     STGAAKAIGLVMPELKGKLDGYALRVPIPTGSVTDLTADLSKSASADEINAAFKAAAE
                     GRLKGILKYYDAPIVSSDIVTDPHSSIFDSGLTKVIDNQAKVVSWYDNEWGYSNRLID
                     LVALVGKSL"
     gene            7390..>7783
                     /gene="pgk"
                     /locus_tag="VFI55_16870"
     CDS             7390..>7783
                     /gene="pgk"
                     /locus_tag="VFI55_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET7075734.1"
                     /translation="MTSSVHTLSDLLKQGVSGRGVLVRADLNVPLDDHGVITDAGRII
                     ASVPTLKSLLDAGAKVVVAAHLGRPKAGADPKLSLAPVAAALGEELGQHVQLAGDVVG
                     ADALARAEGLTDGDILLLENVRFDPRETS"
BASE COUNT         1329 a         2570 c         2550 g         1334 t
ORIGIN      
        1 ccgacgtcgg ccagccgaac cagaacacca cgacggtggc gtagcccttg cgggccagcg
       61 gccgtaatcc gttggcggcg ttgatgaatt ccacggcggc gcgcagcagc ggccggggcg
      121 tgccgaccgg ccgccgcatc agccggagac ccgggcttgc aattcggccc gcagaatctt
      181 gccggtgctg gcgcgcggaa gttcgtcgag gaccgcgatt tcgcggggga ccttgcagtt
      241 ggcccattcc gtcgctacgc atggcggcgg ccatcttgag ggcctggccg agacgagatg
      301 ccatggctca gcccaggatt gggaatcgac gctgggccgc gagttccttg agggcttgct
      361 gcatcaccga tcgcacgtgc tcgtcgacgt cgtcgacgca cgggtgctcg ccgaattgcg
      421 cggtgatgtc gatcggatcc agcacctgcg tcacgatctt ggtgggcagc ggcagattcg
      481 gcgggatcgc ggcgctgaac ccgaacggga agccgaacga gagcggcagg atgtcgctgc
      541 gcagcaaccg tttcaggccc atccgctcgg cgagccacgt accgcgggac aggtagagct
      601 gggtctcctg cccgccgatg gacacggcgg gcacgatggg tacgccggcg tcgatcgccg
      661 tgctgacgta ccccgtgcgc ccgttgaagt cgatgacgtt ctccgacagc gtgggccgat
      721 acgcgtcgta gtcgccgccg ggaaagacga ccaccacgcc gcccgaccgc aaggccgtcg
      781 ccgcgttctc ccggttggcc cggatgtagc cggtgcgccg gaacagctgc cccgtcggac
      841 ccatgaacag gatgtcgtga ctcagcgtga aaaccggccg gtcgtagccg aacttgtcgt
      901 agaagtcgac gctgaagatc gggacgtcca tcgggaacat gccaccggag tggttaccga
      961 ccaccagcgc cccgccgggc gggaaggaat ccaggccgcg cacctccgac cggaagtatg
     1021 tcttcacgaa cgggcgcatc agagccatca ggcgccgggt caggcccggg tcgaatttgt
     1081 cgatatcccc atccccggtg tccgggctgt cgctgtcggt cacgttcctc cctcgaggcc
     1141 ccccgaggcc gcgttgccgc gtccagttac ccgatggtag cgagcgggca cagggcgact
     1201 ttcaggtgtt tcgtctgagt tcggcgccga tcgggtattg cccgggggtg cgcgccgccg
     1261 cacgacgact tcgctgaggt gtctgggacc cgaatgcggg caccgccgcc atcgggcagc
     1321 gttgtgccgc gcgcacaaaa cgggcgctga ggttgatcgt ccgcgcatat ggtgaccgtc
     1381 gggatcccgg ggttagtgtt acccacgctc aacgcccatc ctgcggaaag agaccagatg
     1441 gacttcgccg gcttgtccga agaccgtgct gcacgcccca tcccagggca caggttgcat
     1501 ggcgcatcgg accggcgtgg gcgcagacct cgcagcggct cggcccgtcc cggaccggtc
     1561 gacgtacgtc aaggaggggc catggcttcg ctgattttga accgccccgc cgcccgcgtc
     1621 gagtgctcgg tcaaggcggc tgacacggcg gattaccggc aagccgagta cttcctgggt
     1681 ctgctcgctc agaaccgcgg tcacattgac caccgcatcg gtgactaccg caaggcgatc
     1741 gccgccgcag aggccgtcgg cgacagcgaa gcggccattg gcttgcgccg catggcgcgt
     1801 atcgaagaac aggaccggca caccgttgac gtgctgatcg cgaacctgcg ccggcgattc
     1861 ccgttggcgg tgccgggcga ggttcccccg gtccctcgga gggcgcggct cgcggtccgt
     1921 tgacggcgga gatccaccgg acggattgac gcaagcatgg caaaccagga cctcgtcaat
     1981 accggtcaat accggcatct tcgcggtgcg gtggcgatag attgaagtgg ccatcaggtt
     2041 ccagctccga taggaacgag gccgctcatg ggattcatgt caccggaact gccggacgtc
     2101 gaccctgaca cgtggcaggc ccggccacgg gcagcgcgac tgcaaatcac gacccggcac
     2161 tgggtggaac acggcttcgg aaccccgtac gcggtgtatc tgctttacct gatcaagatc
     2221 ggcgtttacg tcgcggctcc cgctgcgatc atctcgctga ccccgggcct gggcgggctg
     2281 ggccgaatcg gcgactggtg gacgcaaccg atcgtgtacc agaagttcgt catcttcacg
     2341 ttgctgttcg aggtcctggg cttcggctgc gggtccggcc cgctgaccgg gcggttcatg
     2401 ccgccggtcg gcggtttcct atactggttg cgccccaaca cgattcggtt gccaccctgg
     2461 ccgggcaagg tcccgctgac cggcggtgat acccgtaccg tggttgacgt tgccctgtat
     2521 gcggtcgtgt tgatcggcgg cgcgtgggcg ctgttgtcgc ctggtcaggg tggaccggtc
     2581 actgccacag gcgatgtcgg tttgatcgac ccggtactgg tggcgccgcc gatcgtcgcc
     2641 ctggcgattt tgggtttgcg cgacaaaacg atctttctgg cggcccgcgg tgaacactat
     2701 tggctgaagt tgattgtgtt cttctttccc ttcaccgatc agatcgcggc cttcaagctc
     2761 atcatgcttg gcttgtggtg gggggcagcg acttccaagc tgaaccacca ttttccctac
     2821 gtcgtctcgg tgatgacgag caacaacgcg ctactgcgca gcaggctttt caatccgctc
     2881 aagcgcatgc tctacgtcga cccggtcaac gacctacggc cttcctggat ccccaaggtt
     2941 atggcccatg tcggcggtac cacagcggaa ttcctggttc cggggactct ggtgttgata
     3001 gccgacggac aaccgtggcg atggttcctc atcgggttca tggtgatctt ccacctcaac
     3061 atcctgtcca acctcccaat gggagttccg ttggagtgga acgtgttctt catcttctcg
     3121 ctgttctatt tgttcggtca ctatggcgcc atccacgccc tcgacctgcg gtcgccgctg
     3181 ctgttggcca tcttgatagt cgcgctggtg atcgtgccga tcacgggaaa ccttttcccg
     3241 caaaagattt cgttcctgcc agcgatgcgc tactacgccg gcaactgggc gacgagcgta
     3301 tggtgcttcc gcgccggtgc cgaagacaag atagaagcca acatcgtcaa aagctcggcg
     3361 ctggtcatca atcaattggc caagctctac gatgcggaca ccgccgagat catgagcgac
     3421 aagacggcgg cgtttcgcgc gatgcacact catggcaggg cgctcaacgg gttgttgccc
     3481 cgcgccgtgg ggggtgaggc caacgaggcg gactacagcg tgcgggaggg cgaaatcgtt
     3541 gccgggcctt tggtcgggtg gaatttcggc gaaggccatc tgcacaacga gcagctgctg
     3601 gaggcggtgc aacggcgctg ccacttcgag gagggcgacg tgcgcgtcat cgtcctggag
     3661 ggccagccga ttcacatcca gcggcagtgg tatcggatcc tcgatgcgaa gacggggctg
     3721 atcgaggcgg gttgggtcga cgtggcggac atgttaaccc gccagccgtg gcccgagccc
     3781 ggtgacgcgt tccccgtcca cgtcaccagg caacacgtca cacgatgacc accgcggttg
     3841 cggcgatggc tcgcggccgg ctgtagccga aacgtgactg agatttgatc gaatcgctca
     3901 cgcgcccttg catttccact ggtcgtagca cccataccat cactattgca actctgtaaa
     3961 gcagaaggaa ttgaagccat gcgtgcggtt gttcggtgcg tttctgcggt tatcggggtc
     4021 gctgcgaccg tggttccggg gccacccggc gtaagcctgg ccgcggcgaa ccagtcgcac
     4081 ggcttcgcgg tcgcgtcggt tctgccgaca cgcggcgagg tggtgggtgt ggcgcaccct
     4141 gtgatggtga ctttcgttgc gcccgttgac aacttagccg aacggcatgc ggccgagcga
     4201 gccattgagc tcaaatcggc gcccccgatg accggcaagt tcgaatggct cgacaaccgt
     4261 gaggtgcagt gggttcccga ccgattctgg ccggcccaca gcacggtggc gctgtcggtg
     4321 gacggtgagt ccacggaatt caaaacgggt gctgccgtgg tcggtgttgc cagcatctcc
     4381 gagcacacgt tcaccgtgac catcgacgga gtcgaggcga caccgccgcc gccactgccc
     4441 gcgccgcacc accggcccca ctggggagaa gaaggggtga tgccggcgtc gatgggcagg
     4501 ccggagttcg cgacgcccgt cggctcctac accgtcttgt ccaaggaacg ctcggtgatc
     4561 atggattcga gcagcgtcgg catccccgtc gacgatccgg acgggtaccg catcccagtg
     4621 gattacgccg tgcgtatcac cagccgcggc ctcttcgtgc atgcggctcc ctgggcggtc
     4681 aattcactcg ggttcgacaa tgtcagccac ggctgcatca gcctgagccc tgaagacgcc
     4741 gagtggtact acaacaccgt caacattggc gacccggtca tcgtgcagga ataacggcgc
     4801 aggaataact gcgccgacag ccacggcctc gagaggctcc gcacctagac tcggcaccga
     4861 agcgatgtgc ggccgcgtgc cccgggaggc gctgcgggct gcccgtcggt gactcccgga
     4921 tcagggcata acccgacgac gggacgggcc cgccgggtgg tcacccggcg ggcccgcacg
     4981 atcgggttag cgggtcgggc tcagcccgcg ccccggcaac ctacgaggga ccgggcgcaa
     5041 tgggctgacc ggcgactggt ccaccggtcg gcgccggacc aatcgggtca cctttgcccc
     5101 gatagcttcc ggacatgtcg atgagtggag cgccaatcgg cagcggggca cccaccggaa
     5161 cgccggccag caccggcgcc cccaccggca ccgcagcacc tatcggcact ggtgcaccta
     5221 tcggcactgg cgctccgatc ggcactggcg caccgatggg cactggcgca ccgatgggca
     5281 ctggtgcacc gatgggcact ggcgctccga cgggcacggg cgctccgacg ggtatgggtg
     5341 ctccgacggg cactggtgcc cccaccggca ccggcgccgc cgccggcacc ggcggcccga
     5401 gcgcaatcag cggagcgcca accgggacca ccggaaccgg cccaggcgca accagcggaa
     5461 caccagccat gtcggtgaga ggagcgctgg ccgtgcatac gtcgccgacc gttaccggtg
     5521 ctccggcggg tgtcggtcct ccggccgctg ccggtcctcc cgcgggtgcc ggtccgcccg
     5581 cggcggcggg tcctccggcg cctgccgccc cggcggtggc tgcctgtgct ccagcggctg
     5641 cgggtccgcc ggctgtcgcc ggtgcggtgg cgcagcggca ggcggccgcc gccccgccgg
     5701 cgcccgccgc tcctccggcg gccgccagcg ctgcggtatc acccgccatc ccctggacac
     5761 acgcatagcc gccggttttc agcggggcgg ccgccgcatc cgggctcaac gctatagcgg
     5821 ccccgcacaa tccagcgagg gcaactactt tgatgttgcg ccgatcagag accgtcatcg
     5881 acgtcgactc ctttagcagt cagtgtttcc gcaatcaaat ggccagcagc accgcatagc
     5941 cgtatcgcgg tcgtgtcgaa tgtagggctg gtcaatgctg ttagacatgc gacgattgcg
     6001 gcgcttatcg aaccgataca aaggagacat cctccggtta ccgccgctgt ttgcgccccg
     6061 gtcaggcgag gtcaacggcg ccacaacacc ccagcccgtc ccggtcacgt cgtgcggtcc
     6121 ggacgcacgg gcagaaaatc gttcggtggc agggcatgtg ggcgtcgtca cataggtccg
     6181 tcgagcccat cgatgccctt cgctcatatc cgtcgcaacc cgcactcacc ccacccggtt
     6241 tttcgctgcg gcagcaagtc gaccactctt accggcgcgc aaccgaggtg attactgatc
     6301 taggctgacg acgagcgctt atgagcggac acaggaagac agggagagac acgtgacggt
     6361 ccgggtaggc atcaacggct tcggtcgaat cgggcgcaac ttctaccggg ccttactggc
     6421 tcaacaggcg cagggcttgg gcagttccgg ggccgacatc gaggtcgtcg ccgtcaacga
     6481 catcactgac aacgccacgc tggcgcacct gcttcgattc gactcgatcc tgggccggct
     6541 gccctacgac gtcagcctcg aaggtgacga caccatcgtc gtcggtaccg cgaaaatcaa
     6601 ggcgctcgag gtaagagagg gcccggcggc aacgccgtgg gcggacctcg gcgtcgacgt
     6661 ggtcgtggaa tccacggggc tgttcaccga cgcggccaag gccaaaggtc acttggaggc
     6721 cggcgccaag aaggtgatca tctcggcacc cgccaccaac cccgacatca ccatcgtcct
     6781 gggcgtcaac gacgacaaat acgacggcag tcagaacatc atctccaatg cctcctgcac
     6841 gacgaattgc cttgcgccac tggccaaagt gctgcacgac gagttcggca tcgtcaaggg
     6901 tctgatgacc accatccacg cctacaccca ggaccagaac ctgcaggacg gaccgcacaa
     6961 ggacctgcgt cgcgcgcgcg ccgccgcgct gaacatcgtg ccgacttcca ccggtgcggc
     7021 caaggccatc gggctggtga tgccggagct gaagggcaag ctcgacgggt acgcgttgcg
     7081 ggtgccgatt cccaccggct cggtcaccga cctgaccgcc gatctgtcca aatccgcgag
     7141 cgccgacgag atcaacgcgg cattcaaggc cgccgccgaa ggcaggctca agggcatcct
     7201 gaagtactac gacgcgccga tcgtctccag cgacatcgtc accgacccgc acagttcgat
     7261 tttcgactcc ggcctgacca aggtgatcga caatcaggcc aaggtggtgt cctggtacga
     7321 caacgaatgg ggttactcca accgcctcat cgatctggtc gcgctggtcg gcaagtcgct
     7381 gtaatcgtga tgacgtcctc cgtccacacg ctctcggacc tgttgaagca gggggtttcg
     7441 ggtcgtggcg tgctggtgcg cgccgacctc aacgtcccgc tcgacgatca cggcgtcatc
     7501 actgacgccg ggcgcatcat cgcgtcggtt ccgacgctga agtcgcttct cgacgccggc
     7561 gccaaggtgg tggtcgccgc acacctgggt cgtcccaagg ccggcgccga tccgaagttg
     7621 tcgctggcgc ccgtggcggc ggcgctcggc gaggagctcg gccagcacgt gcagttggcc
     7681 ggcgatgtcg tcggcgccga cgcgctggcc cgcgccgagg ggctgaccga cggcgacatc
     7741 ctgttgctgg aaaacgtccg cttcgatccc cgcgaaacca gca
//
LOCUS       DASSGQ010000385        22170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_120822,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000385 DASSGQ010000000
VERSION     DASSGQ010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 22170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22170
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120822"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2068
                     /locus_tag="VFI55_16875"
     CDS             <1..2068
                     /locus_tag="VFI55_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HET7075735.1"
                     /translation="PATAIEERMCAIFARLFGWERVGAEDSFFALGGHSLLAARLVAQ
                     IRAEFSVECNVRAVFDAPTPAGLARLVERFRAGVDADINSADAADLSEPQARRPELGE
                     AIRPERPPLSYSQLAMWFQYRMRGANDVFNMALALRFTGPLDAAVLTRALNDVVARHE
                     ALRTNFVEHEGVPYQHVHPTLEFEMATAEFTAEFAAEEIDEAIAELRRHAFTLESEPL
                     VRPTLLTLGPDSHVLLLLVHHIVTDHTSLGVLFEDLVDAYRARLAGKAPQWATQPLQF
                     ADYAVWQRNAFDPPSDWGKAELTYWRDVLAALPFDISVVPDHTRPPVLGRRGEVVNFT
                     VSADRRAALTRLAEQHGTTEFTVYQAVLAVLLHNFGGGTDIAIGSPAASRVDLATANL
                     IGPSANVVVLRTDLSDDPSPRTAVARSRDTVLNALAHQELPIERLVEALNPPRSPSRN
                     HPLFQNSIHFRGEDWALLPRDLTPDGETTVSPLPMDFEISMLDLNVGMNVTPDGALDV
                     RVVANADLYEPRTVGLVAAALDAAFDAFATTPDSPVSTLELLPAADLEALLAPPTPTD
                     VPRSQPVTGGSAETERALIALLEELLDITGVDLEDNFFAIGGDSIISIQLAARATAQG
                     LNLTPAMVFEHMTIAELAAAVDAASSRPVAESDSAPERQSVPMSASGLDTDALAELNA
                     SWLRQS"
     gene            2076..6590
                     /locus_tag="VFI55_16880"
     CDS             2076..6590
                     /locus_tag="VFI55_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET7075736.1"
                     /translation="MTTAPPQIEDVLALSPLQEGLFALSRLAEDSIDLYTMQLRVDID
                     GPVDVELLRRSAAAMLDRHPNLRAAFWDRDVPKPVQIVPARAELPWTERTATPAEFDS
                     IARSERRRPFELSRGPALRVVVLSVPGETRRRMIFTAHHILMDGWALAVFFTEMLAVY
                     RAGGSPHALPAPRPYRDYILWLAQQDRPAAVAKWTHYLGGVSAPLMVADGTVVARDGV
                     PEQTELLLSEADTARLRNWAGRNGLTLNTVVLFAWAVVLSRLADRRDVVFGTVVSGRP
                     EDLAGVESMVGLFINSVPVVHQVSGTVSVVEQCTQLQREASAMRDIGYLNLSELQRAH
                     GRGTLFDTLFVFENAPVSEAIRTVTTPDGARFSPVEMESLTHYPLTVVAHLRGDALVV
                     LVEAIHEALPHLPAAQICERLRGVLLQLPDIGDETPDVLDVLTATERAEFCELATRSV
                     PLAEGTVWEMFERQVHATPDADALTAGSGERYSYAELREQACRLARELADHGVGPETV
                     VALALPRSTRSIVAILAVLAAGGAYLPIDIALPEGRIESIVSQANPVLVIASAGCAGL
                     VGARIPTLIIDDPIVAERISRRPAAAPVVHRHPQQCAYVIFTSGSTGEPKGVIGTNAA
                     ILSYFADHRERFYRNGLGRPMRIAHAWSLSFDASWQPMVGLLDGHCLHLFDAEEMRDA
                     DRLVEGIAGYRVDMIDTTPSMLGQLRTAGLLERGLAVLALGGEAIDSTLWERLRELSP
                     TAVYNCYGPTEMTVEAVVGRVKDYPAPTIGTTNAGTFGYVLDSALRLVPNGVVGELYL
                     SGAQLARGYVSQSALTAASFVADPFRPGQRMYRTGDLVRRLPHGGYAYLGRGDSQVKI
                     RGYRVEIGEIEAALRRRPEVRDAAVSVLRREESTALVGFVVWQRGVDANPERLRTSLT
                     EDLPSYMIPARVVGLSELPVNANGKLDGHALDRLAQDVISQTTGGAVVAPTETERLLC
                     EVFREQFNGVVPHLDDDFLSFGLDSIVAISLVHKARRHGLALSPRMVFAAPTIRQLSA
                     AIDARLGADTAIANDEYGEVLPLPIVSWLYEYGNYRRFTNSVLLRLPAEIDRSSIELM
                     LQLLLDGHDTLRSTLADTPAGPRLITREPGVVVAADVLTRVQLPSDAELGSTITAAAR
                     RAMDKIDPHAGAMVQAVWFSGAESGEVLLLTVHHLAVDVVSWHVMLGDIAAAWRSVKA
                     GATPKTLPEFTSYRRWSELMWERAATPEVRAQRGYWISQLREPDSALGVRHPDPTRDT
                     WSSLRVAEVLTPVADTERVLGTLTRDEGLREFLLAVTTMAIASWRRERAQDPAAGALV
                     ALDGHGRADSVLDTDTTNTVGWFTTAFPVRLGAGAAAVDIDQAEDDPGAARALFDSVA
                     AHLAAIPNDGLDYGLLHYVDRVPQLQEAAEPQIMFSYLGRLDLSGAADQPWSPLTGSH
                     ISALPVDPEPDLPLRFALYISAHVGGTPEGSQLITSVLWSDALFTPSDIDRLTHFWQR
                     GIAVLARGLA"
     gene            6612..10124
                     /locus_tag="VFI55_16885"
     CDS             6612..10124
                     /locus_tag="VFI55_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020725933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET7075737.1"
                     /translation="MEPVGNERISRDEIRATVAAQLDCPADAVADDDDLIRLGLNSIR
                     MMALAGSWRKRGADVTFAQLAATPTIDSWYGLLGTDEQLGIAEPRTAEPAESSTEEAP
                     YPLATMQHAYWIGRSDEQELGGVAAHLYVEFDGEAIDPERLQGAVSDLVAAHPMLRTR
                     FLPDGTQQTMAKPGRPVFAVVDLRGQSSASIESALAELRDRKTHQRLGIEDGQVIDVT
                     LTRYDGDRTRLHLDIDMLAGDAMSYRVLVSDLAESYRGAALTAPGYSYRRYRTERRVD
                     TTARERDRQWWQQRLAEMPGAPELPTVPVGDRRTPHRTVRYHHWLAPEARQRLTAAAH
                     ARGVTPAMALAAVFADTIGGWSAQSRFLMNVPLFQRESVHHDIDRVVGDFTSSIMLDV
                     DVTENVSVAQRSRTIQRSMYESGSHAAYSGLEVLRDLGRFRGEPVLAPVVFTSALDLG
                     ELFCDNVTQTFGEPVWIVSQGPQVLLDAQVTELRGGLLLNWDVRESAFPQGLIDSMFA
                     CFTETVERLAEGDAGWEAEAAVRLPSAQAQVRAAINATDGPVSGRCLHQGFFEHARTN
                     PDAPAVVWGLGDAPGDIDGAWSYGELAEGALAVAGALRARGVRRGDTVAVQLPKGRDQ
                     ILAVLGVLAAGASYVPIGFDQPDGRRVKILQTADVVAALTVEGSDMGRGISCLSIGAA
                     RACAQPLEAPDFPDTGEIAYVIFTSGSTGVPKGVDVTHGGAMNTVDAVNEWFEVGSAD
                     RVLALSALEFDASVYDIFGMFSVGGSVVAVDAEQRAAATMWVDLLRHHRVSILNCVPS
                     MLDMILELGGDRLGDSVRAVTLGGDWVDADLARRLRKQVPGCRFSGLGGATETSIHNT
                     ICEVAGEPPEYWATVPFGVPLRNVRCRVVAPSGRDCPDWVAGELWVGGANVAAGYRND
                     AQKTAERFVRHDGIRWYKTGDMARYWPDGTIEFLGRADNQVQIRGYRVELGEVETALR
                     TVPGVRHAVAAVVGGNAPKLVAAVAADPAEVGDIAAAVSDLLPRYMIPTRTAFLERIP
                     LTPNGKLDRRAVVALLENQADDALGAHARAPRNDLEAALAGIVAEVLGGTEHASVGVH
                     DDFFALGGDSVLATSVIARVRDWLEIDHAVVADLFATRTVAGLADRLQTREVARGTPE
                     RLTMIARHYLEIAALTDEEVLAEG"
     gene            10341..10880
                     /locus_tag="VFI55_16890"
     CDS             10341..10880
                     /locus_tag="VFI55_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020786783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075738.1"
                     /translation="MTMKSIATGVLAVAAIGAAAAGVTSPASMTPVPVQLAAGGAPYG
                     PASPASGWYPQPQDPPPVPGASTPTSDQLTGLLNSLADPGVPFANKSNLVEGGISGTE
                     AHLADHELKKAAKNGDLPLTFNVTNIQPGAPGAATADVAVSGPKPPSPVTQNVTFVNQ
                     GSWMLSRASAMELLQAAGH"
     gene            10911..11402
                     /locus_tag="VFI55_16895"
     CDS             10911..11402
                     /locus_tag="VFI55_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075739.1"
                     /translation="MKSVTTSLAALAVIGGAAAGIASVAPSSDLSAVQLAAIGAPLPQ
                     DPPPGLPSPDQLSGLCNQATDPGVSYTTKDNLVQGGIGPEEGHVADHDLRKAYREGKF
                     PEQFAVTNIQPAGPNAVTADVAITGPKFAGPVTKNLGFVNEGGNWILQHDSAIGLIQA
                     ATG"
     gene            complement(11399..12268)
                     /locus_tag="VFI55_16900"
     CDS             complement(11399..12268)
                     /locus_tag="VFI55_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7075740.1"
                     /translation="MPDGALTARFEAARPQLGAIAYRMLGSIDDAQDAVQEAWLRLSR
                     ANAGIANVDAWLTTVVARICLNTLRDRHARGREEPVAHLPDPIVDVEGEFDPEHRAML
                     ADAVGLALFVVLDTLPPAERLAFVLHDVFAVPFDQIAPIVDRTPEATRKLASRARRRI
                     EQAHPAPDGDMAAQREAVDAFFAAGRTGDFDRLVSVLHPDVVLRGDFGPDAVFRTEGA
                     SAVAKLARSYAGHEREARAATINGAAGAVIFVGGRATAIMGFVVRAGRVTAVDVLADP
                     ARLARIDLSAVTG"
     gene            complement(12280..12888)
                     /locus_tag="VFI55_16905"
     CDS             complement(12280..12888)
                     /locus_tag="VFI55_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7075741.1"
                     /translation="MPRLNGVSDRDAGPGAKIAFFFTKRKLAKMTGLETAGMLEPLRM
                     YAHIPRLLNAYGRLEQAESKLDILSPRHRALAELKSATTVRCEYCIDLGSQIARQWGL
                     TDEELLALSDYQNASCFSDLDKLILEYATAISRTPVEVSDRLFDALRAHFDTPQLVGL
                     THVITLGNLRARFNVALDIGSSGFSGNRVCALPDAFPDAGRR"
     gene            complement(12985..13332)
                     /locus_tag="VFI55_16910"
     CDS             complement(12985..13332)
                     /locus_tag="VFI55_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HET7075742.1"
                     /translation="MIFKGVRDGKPYPDHGLSYKDWSHIPPQQIRLDELVTTTTVLAL
                     DRLLSEDSTFYGDLFPHAVRWKGITYLEDGLHRAVRAALRNRVVLHARVFDMEVPLSQ
                     QTHVDRDSRTRRF"
     gene            13487..14530
                     /gene="hrcA"
                     /locus_tag="VFI55_16915"
     CDS             13487..14530
                     /gene="hrcA"
                     /locus_tag="VFI55_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET7075743.1"
                     /translation="MGSADDRRFEVLRAIVADFVATKEPIGSKSLVERHNLGVSSATV
                     RNDMAVLEAEGYITQPHTSSGRVPTEKGYREFVDRLDDVKPLSSAERRAIQSFLESGV
                     DLDDVLRRAVRLLAQLTRQVAVVQYPTLSTSTVRHLEVVALSPARLLMVVITDSGRVD
                     QRIVELGDVIDDHQLSQLREMLGQALVGKRLSVASTAVADLAGDLVGRSGLASNLSNA
                     VGRSATVLLESLLEHTEERLLMGGAANLTRNAADFGGSLRSILEALEEQVVVLRLLAA
                     QQQAGKVTVRIGHETAVEQMVGTSMVSTAYGSSDTVYGGMGVLGPTRMDYPGTIASVA
                     AVALYIGEVLGAR"
     gene            14603..15745
                     /gene="dnaJ"
                     /locus_tag="VFI55_16920"
     CDS             14603..15745
                     /gene="dnaJ"
                     /locus_tag="VFI55_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET7075744.1"
                     /translation="MARDYYGLLGVGRNASDADIKRAYRKLARELHPDVNPDEVAQAK
                     FKEISVAYEVLSDPEKRRIVDLGGDPMENAASAGGFGGFGGLGDVFEAFFGGGFSGGA
                     ASRGPIGRVRPGSDSLLRMRLDLEECASGVTKQVTVDTAVLCDRCQGKGTNADSVPVP
                     CDTCGGRGEIQTVQRSLLGQMVTSRPCPTCRGVGVVIPDPCHQCVGDGRVRARREISV
                     KIPAGVGDGMRVRLAAQGEVGPGGGPAGDLYVEVHEQAHDIFVREGDDLHCTVSVPMV
                     DAALGATVSVEAILDGTTEITIAPGTQPGSVITLRGQGMPHLRSSARGNLHAHVEVVV
                     PTRLDHHDTELLRELKNRRGRDVPEVRSTHAGGGLFSRLRETFTGR"
     gene            15771..16583
                     /locus_tag="VFI55_16925"
     CDS             15771..16583
                     /locus_tag="VFI55_16925"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET7075745.1"
                     /translation="MVATLFYVDALPDTGALAVVDGDEGFHAATVRRIRPGEELVLGD
                     GAGGLARCQVERAGRDGLHARVLGRWSVAPGRPAVTVVQALPKSERSELAIELATEAG
                     ADAFLAWQAARCVATWEGARVDKGLRRWRAVARSAARQSRRAHIPPVEGVLSTAGLIQ
                     RVRDEVAAGAAVLVLHESATDGLADVFPDIAGTQANSLLLLVGPEGGIGPDEIAALTE
                     AGAVAVRLGPQVLRTSTAAAVALGALGVLTPRWDVPVESRNTESEPNDGMHR"
     gene            16682..17740
                     /locus_tag="VFI55_16930"
     CDS             16682..17740
                     /locus_tag="VFI55_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET7075746.1"
                     /translation="MTPREISAAAASGALQADAQVRSSIDVPPDLVVGLLGSADENLR
                     ALERTLNADLHVRGNAVTLSGEPADVALAERVISELVAIVAGGQPLTPEVVRHSVAML
                     VGTGNESPAEVLTLDILSRRGNTIRPKTLNQKRYVDAIDANTIVFGVGPAGTGKTYLA
                     MAKAVRALQTKQVSRIILTRPAVEAGERLGFLPGTLSEKIDPYLRPLYDALYDMMDAE
                     LLPKLMSAGVIEVAPLAYMRGRTLNDAFIVLDEAQNTTAEQMKMFLTRLGFGSKIVVT
                     GDMTQIDLPGGARSGLRSAIDILENVEDIHIAELTSVDVVRHRLVSEIVDAYARYEEP
                     GSGMNRAARRASGVRNRR"
     gene            17803..18336
                     /gene="ybeY"
                     /locus_tag="VFI55_16935"
     CDS             17803..18336
                     /gene="ybeY"
                     /locus_tag="VFI55_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET7075747.1"
                     /translation="MSIEVSNESGIDVSEAELVSVARFVIASMDVNPAAELSMVLLDT
                     AAMADLHMRWMDLPGPTDVMSFPMDELEPGGRPDAPEPGPSMLGDIVLCPEFAAEQAA
                     AAGHSLGHELALLTIHGVLHLLGYDHAEPDEEKEMFGLQERLLEEWVAEQVEAYHNVL
                     QEERDRRLLDKSRYFDN"
     gene            18333..19649
                     /locus_tag="VFI55_16940"
     CDS             18333..19649
                     /locus_tag="VFI55_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET7075748.1"
                     /translation="MTGLSQLFGAIALVALGGLFAAIDAAISTVSLARVQELVRDGRP
                     GAVSLCKVVTERPRYINLMVLLRLTCEITATVLVVVFLYDNFGMSWALFGTATTMVVV
                     SFVVIGVGPRTLGRQHAYPIALATALPLRAISWLLMPLSRLLVVLGNAVTPGRGLRNG
                     PFASEIELREVVDLAQQRGVVAADERRMIQSVFELGDTPAREVMVPRTEMIWIESDKL
                     AVQATNLAVRSGHSRIPVIGENVDDVVGVVYLKDLVQQTFLSADGGQDTTVSHVMRPA
                     VFVPDSKPLDALLREMQHDRNHMALLVDEYGAIAGLVSIEDVLEEIVGEIADEYDQAE
                     TAPIEDLGDKCFRVSTRLPIEDVGELYGVEFDDDLDVDTVGGLLALELGRVPLPGSEV
                     VSHGLRLRAEGGTDDRGRLRVTTVLLSPAESDSLDGPNGGEPNQHD"
     gene            19621..19962
                     /locus_tag="VFI55_16945"
     CDS             19621..19962
                     /locus_tag="VFI55_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET7075749.1"
                     /translation="MVGSPINMTEQLAAEDAKLVVLARAAMARAEADSAAAVRDVDGR
                     TYAAAPVDLPALKLTGLQAAVAAAASSGATGLAAAVLVAASADDPGIEAVRKLSPTAV
                     IIVTDRAGVVL"
     gene            19965..20873
                     /gene="era"
                     /locus_tag="VFI55_16950"
     CDS             19965..20873
                     /gene="era"
                     /locus_tag="VFI55_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET7075750.1"
                     /translation="MTEFRSGFVCLVGRPNTGKSTLTNALVGTKVAITSTRPQTTRHT
                     IRGIVHRENFQMILVDTPGLHRPRTLLGQRLNDLVRDTYAEVDVIGLCIPADEALGPG
                     DRWIVDQIRSVARTATVQKLVVIVTKIDKVPKDRVAAQLMAVSELVNDSAEIVPLSAV
                     TGEQLEVLIDVLAAALPPGPAYYPDGELTDEPEEVLMAELIREAALEGVRDELPHSLA
                     VVIDEVSPREGRDDLIDVHALLYVERDSQKGIVIGKGGARLREVGTAARAQIEKLLGT
                     KIYLDLRVKVAKNWQSDPKQLGRLGF"
     gene            complement(20912..>22170)
                     /locus_tag="VFI55_16955"
     CDS             complement(20912..>22170)
                     /locus_tag="VFI55_16955"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET7075751.1"
                     /translation="TAPLLGIPIAVKDDVDVAGVPTAFGTEGYVAPAAHDAEVVRRLK
                     AAGAVIVGKTNTCELGQWPFTSGPGFGHTRNPWSRRHTPGGSSGGSAAAVAAGLVTAA
                     IGSDGAGSVRIPAAWTHLVGIKPQRGRISTWPLPEAFNGITVNGVLARTIADAALVLD
                     AASGNVEGDRHKPPPITASDYVGIAPGPLNIALSTRFPYTGFRAKLHPDILTATRAVR
                     RQLELLGHTVVPGNPDYGLRLSWDFLARSTSGLRDWEERLGDGVILDPRTLSNLRMGH
                     VLGQAVLRSARRHEAASQRRVGSIFDIVDVVVAPTTAQPPPLARAFDRLGGLGTDRAM
                     IAACPLTWPWNVLGWPSINVPAGFTSEGLPIGVQLMGPANSEGLLISLAAELEAVCGW
                     STRQPAIWWNAGTDVPPAPSSAPSRR"
BASE COUNT         3594 a         7185 c         7632 g         3759 t
ORIGIN      
        1 accggcgacg gccatcgagg agcgaatgtg cgcgatcttc gcccggctgt tcggttggga
       61 gcgagtcggt gccgaggact cgttctttgc cctgggcgga cattcactgc tggcggcccg
      121 cctggtggcg cagatccgcg cggaattttc cgtcgagtgc aacgtccgcg ccgtgttcga
      181 tgcacccacc ccggcgggat tggcgcgact ggtcgaacgg ttccgcgccg gggtcgacgc
      241 cgacatcaac tcggcggacg cggcggacct gtcggagccg caggcacgtc gccccgaact
      301 cggcgaggcg atccgccccg agcggcctcc gttgtcctac tcccagctcg ccatgtggtt
      361 ccaatatcgc atgagaggcg ctaatgacgt cttcaacatg gcactggcgc tgcgcttcac
      421 cggtccactc gacgctgctg tactcaccag ggcgctcaac gacgtagtgg cccgccacga
      481 ggcgctccgc acgaacttcg tcgagcacga aggcgttccg tatcagcacg tgcacccaac
      541 gctcgagttc gagatggcga ccgccgaatt cacggccgag ttcgccgccg aggaaatcga
      601 tgaggcgatc gccgaactgc gtcgccacgc gttcacgctg gagtccgagc cgctggtcag
      661 gcccactctg ttgacccttg gccccgattc gcatgtgctg ctcttgctgg tccaccacat
      721 cgtcaccgat cacacctcgc tgggcgtgct gttcgaggac ctggtcgatg cgtatcgggc
      781 acggttggcg ggaaaggctc cgcagtgggc gacgcagcct ctgcaattcg cggattacgc
      841 agtgtggcaa cggaatgcgt tcgacccgcc gagtgactgg gggaaagcgg agctgacata
      901 ttggcgcgat gtgctggccg cgctgccctt cgacatctcc gtcgtgcccg accacacccg
      961 cccgccggtc ctgggaaggc gcggggaggt ggtgaatttc acggtctctg cggaccgtcg
     1021 cgcagccctc accaggctgg ccgagcaaca tggcaccacc gaattcacgg tctatcaggc
     1081 cgttctcgcg gtgttgttgc acaatttcgg cggcggcacc gacatcgcga tcggcagccc
     1141 ggcggcgtct cgggtcgacc tggccaccgc gaacctgatc ggccccagcg ccaacgtagt
     1201 ggtgctacgc accgacctgt ccgacgatcc gagtccgcgc accgcggtcg cacgcagccg
     1261 cgacacggta ctcaatgcgt tggcgcacca ggaacttccg atcgaacggt tggtggaggc
     1321 gctcaacccg ccacgctcgc cgtcccggaa tcacccgctg ttccagaact cgatccactt
     1381 ccgcggcgag gactgggcgc tgctgccgcg tgatctcacc ccggacggtg agaccacggt
     1441 ctccccgctt cccatggact ttgagatctc aatgctcgac ttgaacgtgg gcatgaacgt
     1501 gacaccggac ggcgcgctcg acgtgcgggt cgtcgccaac gccgacctct atgaacctcg
     1561 gaccgtcggg ctcgtcgccg cggctctgga tgccgcgttc gacgcgttcg cgacgacacc
     1621 ggattcgccg gtatccacgc tggagttgct gcccgcggcc gacctggaag cgctgctcgc
     1681 cccgccgacc ccgaccgacg tgccgcggtc gcaaccggtc accggcggtt ccgcggagac
     1741 cgaacgggcc ttgatcgcac tgctggagga actgctcgac atcaccggcg ttgaccttga
     1801 ggacaacttc ttcgccatcg gcggtgacag catcatctcc atccaattgg cggcgcgggc
     1861 caccgcccag gggttgaacc tgacgccggc aatggtgttc gagcacatga ccatcgccga
     1921 gctggccgcg gccgtcgacg cagcatccag tcgtcctgtg gccgaatcgg attcggcgcc
     1981 ggagcggcaa tccgtgccga tgagtgcctc ggggctggac accgatgcgc tggccgagct
     2041 caacgcatcc tggctcaggc agtcgtgagg cggtcgtgac cacagctccg ccgcagatcg
     2101 aggacgtcct tgccctcagc ccgctgcagg aagggctgtt cgcgctgtct cgactggccg
     2161 aggacagcat cgacctctac accatgcagt tgcgggtcga tatcgacggg cccgtcgacg
     2221 tggaactgct tcgccgcagc gccgccgcca tgctggaccg gcacccgaac ctgcgcgccg
     2281 cattctggga tcgcgatgtc ccgaagccag tacagattgt ccccgcccgc gccgagctcc
     2341 cctggaccga acgtaccgca acgccagcgg aattcgactc catcgcacgc tccgagcggc
     2401 gtcggccctt cgagttgagt cgcggcccgg cgttgcgcgt cgtcgtgctc tccgtccccg
     2461 gcgagaccag gcgccggatg atcttcaccg cgcaccacat tctgatggac ggctgggcac
     2521 tggccgtgtt cttcaccgag atgctggccg tgtatcgggc ggggggatcg ccccacgcac
     2581 taccggcccc gcgcccgtat cgcgactaca tcctgtggct cgcccagcag gacaggccgg
     2641 cggcagtcgc caagtggacc cactacctgg gtggcgtgtc cgcaccgctc atggtggccg
     2701 acgggacggt cgtcgcgcgc gacggcgtgc cggagcagac ggaactgttg ctgtcggagg
     2761 ccgatacggc gcggttgcga aactgggccg gacgcaacgg cctcaccctc aacacggtgg
     2821 tgcttttcgc gtgggccgtc gtgctgagca ggctcgccga ccgccgcgat gtcgttttcg
     2881 gcacggtggt gtccggccgt ccggaggatc tggcgggtgt tgagagcatg gtcggcctgt
     2941 tcatcaacag cgtgccggtg gtgcatcagg tgagcggcac ggtctcggtg gtcgaacaat
     3001 gcacgcagtt gcagcgtgaa gcctcggcga tgcgcgacat cggatatctc aatctgtccg
     3061 aattgcaacg cgcacatggc cgtggcacgt tgttcgacac cctgttcgtc ttcgagaacg
     3121 cgccggtctc ggaagccatc aggacggtga ccacacccga cggcgcccgc ttttccccgg
     3181 tcgagatgga aagcctcacc cattacccgt tgaccgtcgt cgcccacctg cgaggcgatg
     3241 cgctggtggt gctcgtcgag gcgattcacg aagcgctacc gcatcttccg gcggcgcaaa
     3301 tctgcgaacg tctgcgtggc gtgctgctgc aacttcccga catcggcgat gagacccccg
     3361 acgtgctgga cgtactcacc gcaaccgagc gcgccgagtt ctgtgagctc gcgacccggt
     3421 cggtacccct cgccgaggga acggtgtggg agatgttcga gcggcaggtt cacgccacac
     3481 cggacgccga cgcgctcacc gcaggcagtg gggaacgtta cagctatgcc gaactacgcg
     3541 agcaggcctg ccggctcgcg agggagctgg ccgaccatgg ggtaggcccg gaaaccgtcg
     3601 tggcactggc cctgccgcgt tcgacgcgat ccatcgtggc catcctggcc gtgctcgccg
     3661 ccggcggcgc ctacctgccg atcgacatcg cgctgccgga ggggcgcatc gaatccattg
     3721 tgagccaggc caatccggtc ctcgtcatcg cctcggccgg ctgcgccggg ttggtcgggg
     3781 cgcgcattcc cacgttgatc atcgatgatc ctattgtagc cgaacgtatt tcgcggcgtc
     3841 ccgccgccgc gccggtggtc catcggcatc cgcagcagtg cgcgtacgtc atcttcacct
     3901 cgggctccac tggtgagccc aagggtgtca tcggcaccaa tgcggcgata ctcagctatt
     3961 tcgccgacca ccgggagcgc ttctaccgca acggattggg ccgcccgatg cgtatcgcgc
     4021 acgcgtggtc attgagtttc gatgcgtcct ggcagcccat ggtcggcctg ctggacggcc
     4081 attgcctgca cctgttcgac gccgaggaga tgcgcgacgc ggaccggctg gtggaaggca
     4141 tcgccggcta ccgggtcgac atgatcgaca ccaccccgtc gatgttgggg caattgcgca
     4201 ccgccggact gctggagcgc ggtcttgcgg tgctggccct gggcggcgaa gcgatcgaca
     4261 gcacactatg ggaacggctg cgcgaactgt cgccgaccgc ggtctacaac tgttacgggc
     4321 ccaccgagat gacggtcgag gcggtggtgg gacgcgtcaa ggactatccg gcgccgacga
     4381 tcggcacgac caacgccgga accttcggct atgtgctgga ttccgcgctg cggttggtgc
     4441 ccaacggcgt ggtgggcgag ctctatttgt cgggcgcgca gctggcccgc ggctatgtca
     4501 gccagtccgc gctgaccgca gcaagtttcg tcgcggaccc cttccgtccc ggccagcgca
     4561 tgtaccgcac cggcgacttg gtgcgtcgcc tgccgcacgg cgggtacgcc tatttggggc
     4621 gcggcgacag ccaggtgaag attcgcggct accgtgttga gatcggcgag atcgaggccg
     4681 cgctgcgtcg tcggcccgag gtgcgcgatg cggccgtctc ggtgctgcgc cgcgaggagt
     4741 ccaccgcctt ggtgggattc gttgtgtggc agcggggtgt ggacgccaac ccggaacggc
     4801 tgcgcacttc gctcaccgaa gatcttccgt catacatgat tccggcccgc gtcgtcgggc
     4861 tatctgaact gccggtgaac gccaacggca agttggacgg ccacgcattg gatcggctgg
     4921 cgcaggacgt gatctcgcag accaccggcg gcgcggtggt ggcgcctacc gagaccgaac
     4981 gattgctctg cgaagtgttc cgggagcagt tcaacggggt tgtgccgcac ttggacgacg
     5041 acttcttgtc gttcggttta gacagcatcg tggcgatctc cttggtccac aaggcgcgcc
     5101 ggcatggtct cgcgctgagc cctcggatgg tgttcgccgc gccgacaatt cgccaactgt
     5161 cggccgcgat cgacgcgcgg ctcggcgcgg acaccgcgat cgccaacgac gagtacggcg
     5221 aagtgctgcc gttgccgatt gtgtcctggt tatacgagta cggaaactac cgccgcttca
     5281 ctaatagcgt cttgctccga ctgcccgccg aaatcgaccg ttcgtcaatc gaattgatgc
     5341 tgcaactgtt gctggatgga cacgacactc tgcgatcgac gctggctgac acccccgcgg
     5401 ggccacgcct gatcactcgc gaacccggcg tggtggttgc cgccgacgtg cttacccgtg
     5461 tgcagctacc gagtgatgct gaattgggct caaccattac agctgccgcg cgacgggcga
     5521 tggacaagat tgacccgcac gcgggtgcga tggtgcaagc ggtgtggttc tccggcgccg
     5581 agagcggtga ggtcctgttg ctcactgtgc accacctggc ggtagacgtg gtttcctggc
     5641 acgtcatgct gggtgatatc gcagcggcat ggcgctcggt gaaagccggc gcgacgccga
     5701 aaacactgcc agagttcact tcttaccgcc ggtggtcgga gctcatgtgg gaacgggcgg
     5761 ccactccgga ggtgcgagcc cagcgcggct actggatctc acagcttcgt gagcccgatt
     5821 ccgcattggg tgtgcgacat ccggacccga cccgtgacac ctggtccagt ctgcgagtag
     5881 ccgaggttct caccccggtt gccgacaccg agcgcgtgct cggcactctc accagagacg
     5941 agggtctgcg cgaattcctg ctggccgtaa ccacaatggc cattgcgagt tggcgccgag
     6001 agcgtgcgca ggatccggct gcgggcgcgc tcgtcgcgct ggacggccat ggccgcgctg
     6061 actccgtgct ggacaccgac accactaaca cggtcggttg gtttacgact gcgttcccgg
     6121 tgcggcttgg ggcgggtgcg gcggccgtcg atatcgatca ggccgaggat gatccggggg
     6181 cggcccgggc actgtttgat tcggtggccg cccatcttgc cgcgatcccg aacgacggtc
     6241 tggattacgg tctgcttcat tacgtcgacc gcgttccgca gttgcaggag gccgccgaac
     6301 cgcagatcat gttcagctat ctgggtcgtc tggacctcag tggtgccgcg gatcaaccgt
     6361 ggtcaccact caccggatcc cacatcagtg cgctgccggt tgaccccgaa ccagatctgc
     6421 cactgcgttt tgctctctac atcagcgcgc atgtaggtgg tacaccggag ggctcccaac
     6481 tgatcaccag cgtgctctgg agtgatgccc tgttcacgcc gtccgatatt gaccgtctga
     6541 cgcatttctg gcagcgcggc atcgccgtgc tcgctcgcgg gttggcatag acaggcacag
     6601 gagggaccgg aatggagccg gttggcaacg agcggatcag cagggacgag atcagggcta
     6661 ccgttgctgc ccagctcgat tgccctgccg acgcggtcgc cgatgacgac gacctcatcc
     6721 ggttgggtct gaactcgatc cgcatgatgg cgctggcggg gagctggcgt aaacgcgggg
     6781 ccgatgtcac cttcgcccaa ctcgccgcca ccccgacgat tgactcctgg tatggcctgc
     6841 ttggcacgga cgagcagctc ggcatagcgg agccccgcac cgcggagccc gccgaatcgt
     6901 cgaccgaaga agcgccgtat ccgttggcca ccatgcagca cgcgtactgg atcggtcgct
     6961 ccgacgagca ggagctgggc ggcgtcgccg cccacctcta cgtcgaattc gacggcgagg
     7021 caatagatcc cgaacggcta caaggcgcgg tgtccgactt ggtggccgcg catcccatgc
     7081 ttcgtacgcg atttctcccg gacgggacgc aacagacgat ggccaagccg ggcaggccgg
     7141 tgttcgcggt ggtcgacctc cgcggccaga gttcggcgtc gatcgaatcc gcgttggcag
     7201 agttgcgcga ccgtaaaacc caccagcgtc tgggaattga agacggtcag gtcatcgacg
     7261 tcacgctgac ccggtatgac ggggaccgca cccggctgca cctcgacatc gacatgctgg
     7321 ccggcgacgc gatgagctac cgcgtgctgg tctccgatct cgccgagtcg taccgcggtg
     7381 cggccctgac cgcgcccggc tacagctacc ggcgttatcg caccgagcgc cgggtggaca
     7441 ccacagcacg cgagcgagac cgacagtggt ggcagcagcg gttggcggag atgcccggtg
     7501 cacccgaact cccgacagtt ccggtggggg accgccggac gccgcatcgc accgtccggt
     7561 atcaccactg gttggcgccg gaagccaggc aacgcctgac ggccgcggct cacgcgcgcg
     7621 gtgtcactcc cgcgatggca ttggcggcgg tcttcgccga caccattggt ggctggtcgg
     7681 cacaaagcag gttcctgatg aatgtgccgt tattccaacg tgaatcggtg caccacgaca
     7741 tcgaccgcgt ggtgggggac ttcacctcgt cgatcatgtt ggacgtcgac gtcaccgaaa
     7801 atgtctcagt ggcgcagcgg tcccggacga ttcagcgcag catgtacgaa agtggttcac
     7861 acgcagcgta ttccggactc gaggtgctgc gcgacctggg tagattccga ggtgaaccgg
     7921 tgctcgcgcc cgtcgtgttc accagcgccc tggacctggg tgagttgttc tgtgacaacg
     7981 tgacccagac attcggtgaa ccggtgtgga tcgtttctca ggggcctcag gtgttgctcg
     8041 acgcccaggt caccgagctg cgcggtggac tgctgctcaa ttgggacgtc cgtgagtcgg
     8101 cgtttccgca aggcctgatc gattccatgt tcgcctgctt caccgagaca gtcgaaaggc
     8161 tcgccgaggg ggatgcgggc tgggaggccg aagccgcggt gcggttgccg agcgctcagg
     8221 cgcaggtacg cgcggcgatc aacgcgacgg acggtccggt cagcgggcgg tgcctgcacc
     8281 agggtttctt cgagcacgcc cgcacgaacc cggacgcgcc ggccgtggtg tggggtctgg
     8341 gcgacgcacc gggagacatc gatggtgcgt ggagctacgg cgaactcgcc gagggcgctc
     8401 tggcggtggc cggtgcgctg cgtgcgcgtg gtgtgcggcg cggcgacacc gtcgccgtcc
     8461 agttacccaa gggccgcgat cagatcctgg ccgtcctcgg tgtgcttgcc gcgggcgcat
     8521 cgtacgtgcc gatcggattt gaccagccag atggacgacg cgtcaagata cttcagaccg
     8581 cggacgttgt cgccgcgctg accgttgagg gctcggatat gggtcggggt atttcgtgtc
     8641 tgtcgatcgg cgcggcgcgc gcctgtgcgc agccgctcga ggcgccggat ttcccggaca
     8701 ccggcgagat cgcctacgtc atcttcacgt ccggttcgac cggtgtgccc aagggggtcg
     8761 atgtcacgca cggcggggcg atgaacaccg tcgacgcggt caacgagtgg ttcgaggtcg
     8821 gcagcgccga tcgggtgctg gcgctgtccg ccctcgagtt cgacgcctcg gtttacgaca
     8881 tcttcggcat gttctcggtg ggcggctcgg tggtcgcggt ggatgccgag cagcgggccg
     8941 ccgctaccat gtgggtggat ttgcttcgcc accacagggt ttcgattctc aactgtgtgc
     9001 ccagcatgtt ggacatgatc ttggagctcg gcggcgatcg gctgggcgat tcggtgcggg
     9061 ccgtcacgct cggtggtgac tgggtggacg ccgatctggc tcgcaggctg cgcaagcagg
     9121 tgccgggttg tcggttctcc ggtcttggcg gtgccaccga gacttccatc cacaacacca
     9181 tctgtgaggt ggccggcgaa ccgcccgagt attgggcgac ggtgccgttc ggcgttccgt
     9241 tgcgcaatgt gcggtgtcgg gtcgtggcgc cgtcaggtcg cgattgtccc gactgggttg
     9301 ccggtgaatt gtgggtcggc ggcgcgaatg tcgcggcggg ttaccgcaac gacgcgcaaa
     9361 agaccgccga gcgtttcgtc aggcacgacg gtattcgctg gtacaaaacg ggcgacatgg
     9421 ccaggtactg gcccgatggc accatcgaat tcctcggtcg cgccgacaac caagtgcaga
     9481 ttcgcgggta ccgggtcgaa ctcggtgagg tagagaccgc gcttcgcacg gtgccgggag
     9541 tccgccatgc cgtcgcagcc gtcgtcggcg gcaatgcccc gaaactcgtc gccgccgtcg
     9601 cggccgaccc ggctgaagtc ggcgacatcg cggcggccgt ctcagacctg ctgccgagat
     9661 acatgattcc cactcgcacc gcgtttctgg aacggattcc attgaccccg aacggcaaac
     9721 tggaccggcg tgccgtcgtt gcactgttgg agaaccaggc cgacgacgcc ttgggtgccc
     9781 atgcgcgcgc cccgcgcaat gacctcgagg ccgcgctcgc cggcatcgtc gccgaggtgc
     9841 tgggcggcac cgagcacgct tcggtcggcg tgcacgacga ttttttcgca ttgggcggcg
     9901 attcggtgtt ggccaccagc gtcatcgccc gggtgcgtga ctggctggaa atcgatcatg
     9961 cggtggtcgc ggacctgttc gcgacgcgaa ccgtcgccgg cttggccgat cggttgcaga
    10021 cgcgggaggt tgcgcgcggc acgccggaac gcctgacgat gatcgctcgc cactacctgg
    10081 agatcgcggc attgaccgac gaggaggtcc tcgccgaggg ctgacccatg ctggcgacgc
    10141 tgtgtggaaa ccaggtccgg cagtgcggac gacccgtacc gtggcggcac accttggcca
    10201 taccgcaacc gctcggagac ttaaaacggc ggtaacagag cgcacaattg caacacggga
    10261 cacgcggggg cgcagtgccg tgtcagggct actgaaagct actgaaagct attgaaagct
    10321 attgggggaa accaaccgtg ctgaccatga aatccatcgc cacgggtgtg ctggcagtgg
    10381 ccgcaatcgg agccgcggcg gccggcgtga catcgcccgc gtccatgacg cccgtcccgg
    10441 tgcaactcgc cgcgggcggc gcgccttatg gaccagcttc ccccgcaagc gggtggtacc
    10501 cccagccgca ggacccgccg ccggttccgg gcgccagcac cccgaccagt gaccagctga
    10561 ccggcctgct gaacagcctt gccgaccccg gcgtgccgtt cgcgaacaag agcaatttgg
    10621 tcgagggcgg catcagcggg acggaagccc atctcgccga ccatgagctg aagaaggccg
    10681 ccaagaacgg cgacctgccg ttgacgttca acgtcaccaa catccaaccg ggggcgccgg
    10741 gcgcggcaac ggctgatgtc gcggtctcgg gtccgaagcc cccgagcccg gtcacacaga
    10801 atgtaacctt cgtcaaccag ggcagctgga tgctgtcgcg tgcctcggca atggagctgt
    10861 tgcaggccgc gggacactga acggcactaa acggcactga agggaaaacc gtgaaatccg
    10921 taactaccag cctggcggcc ttggccgtca tcggcggcgc agccgcaggc atcgcctctg
    10981 tcgcaccgtc gagtgatctt tctgccgtgc aactggcggc gatcggcgcg ccgcttccgc
    11041 aggacccgcc gcccgggctg ccgagcccgg accagttgtc cggcctctgc aaccaggcca
    11101 ccgatcccgg cgtgtcgtac acgaccaagg acaatctggt ccaaggcggc atcggtcccg
    11161 aagagggcca tgtcgccgac cacgacctgc gtaaggccta ccgggaaggg aagtttccgg
    11221 agcagttcgc cgtgacgaac attcagccgg ccggccccaa cgcggtgacc gcggacgtcg
    11281 ccatcacggg tccgaagttc gccggaccgg tcaccaagaa cctcgggttc gtcaacgaag
    11341 gcggcaactg gatactgcaa cacgactccg cgatcggtct gatacaggcg gcgactggct
    11401 agccagttac cgcgctcaag tcgatccgcg cgaggcgtgc ggggtcggcg aggacgtcga
    11461 ccgccgtgac gcggccggcg cgcaccacaa aacccatgat ggccgtcgcg cggccgccca
    11521 cgaagatgac cgcacccgcg gcgccgttga tggtggctgc ccgcgcctcg cgctcgtgtc
    11581 ccgcgtaact gcgggccagc ttggccaccg cggacgcgcc ctcggtgcgg aacacggcgt
    11641 ccgggccgaa gtcgccgcgc aagaccacgt cgggatgtaa caccgacacc agccggtcga
    11701 agtcgccggt gcgccccgcc gcgaagaacg cgtcgaccgc ttcgcgttga gcggccatat
    11761 ccccgtcggg agcggggtgg gcctgctcga tgcggcggcg cgcccggctg gccagcttgc
    11821 gcgtcgcctc gggcgtccga tccacgatgg gtgcgatctg gtcgaacggg accgcgaaca
    11881 cgtcgtgcag cacaaacgcc agccgttcgg ccggcggcaa cgtatccagc acgacgaaca
    11941 acgccagccc caccgcgtcg gccaacatcg cacgatgctc ggggtcgaac tcgccctcca
    12001 cgtcgacgat cgggtccggc aggtgcgcga ccggttcctc gcggccgcgg gcgtgccgat
    12061 cgcgcagcgt gttcaggcag atgcgggcca ccacggtggt cagccaggca tcgacgttgg
    12121 cgatcccggc gttcgcgcgg ctcagccgca gccacgcctc ctgcacggcg tcctgggcgt
    12181 cgtcgatcga gcccagcatg cggtacgcga tcgcgcccag ctgcgggcgg gccgcctcga
    12241 agcgtgccgt cagtgctcca tccggcatat ccggcgcggt cacctgcgac cggcgtccgg
    12301 gaaagcgtcg ggtagggcgc atacccgatt gccggaaaag cccgacgagc cgatgtcgag
    12361 cgcaacgttg aaacgggcac gaaggttgcc cagcgtgatg acatgggtaa gcccgaccag
    12421 ttgcggggtg tcgaaatgcg cgcgcagcgc gtcgaaaagc cggtcgctca cctcgaccgg
    12481 ggtgcggctg atcgcggtcg cgtactccag gatcagcttg tcgaggtcgg agaagcacga
    12541 ggcgttctgg tagtcggaca gggcgagcag ttcctcgtcg gtgagtcccc actggcgggc
    12601 gatctgcgaa ccgaggtcga tgcagtactc gcagcgcacc gtcgtcgccg acttcagctc
    12661 ggccagcgcg cggtggcggg ggctgaggat atccagcttc gattcggcct gttccagcct
    12721 gccgtacgcg ttgagcagtc gcgggatgtg cgcatacatc cgaagcggtt ccagcattcc
    12781 ggccgtctcc aggccggtca tcttggccag cttgcgcttg gtgaagaaga aggcgatctt
    12841 ggcgcccggg ccggcgtcgc ggtcggacac tccgttgaga cggggcatgg cgatcctcct
    12901 cggaagtcgc ggctggacct tgaccgggtc ctcactacgt cgacacctgt cgacgcacaa
    12961 acgtgaccgc gttgaccgcc gggctcagaa tcggcgcgtg cgcgagtccc gatccacatg
    13021 cgtctgctgg ctcagcggca cctccatgtc gaatacccgg gcatgcagca cgacgcgatt
    13081 ccgcagcgcg gcgcgtaccg cacggtgcag gccgtcctcg agataagtaa tgcccttcca
    13141 ccgcacggcg tgcgggaaaa gatccccgta gaaggtggag tcctccgaga gcagacggtc
    13201 cagcgcaagc acggtcgtcg tggtgaccaa ttcgtcgagc cggatttgct gcggaggtat
    13261 gtgggaccag tctttgtacg acagcccatg gtcgggatac ggtttgccgt cgcgcacgcc
    13321 tttgaaaatc attggcgggt cttccctggt gcgtcgcccg agcccgacga atacatgctg
    13381 gacaggctag tcgggaattc gctcgcggtc gccgcgagag gaccgtaaat ggcagttcac
    13441 accgcttgag accgtaaaat ggatgcgatc aaggaggtgg gagccgatgg gaagtgccga
    13501 cgaccgtcgc ttcgaggtgc tgcgcgccat cgtcgccgac ttcgtcgcca ccaaggaacc
    13561 gatcggctcg aagtccctgg tggagcgtca taacctgggt gtctcgtccg ccacagtccg
    13621 caacgacatg gcggtgctgg aggctgaggg ctacatcacc cagccgcata ccagctctgg
    13681 gcgggtaccc accgaaaagg gctaccgcga gttcgtcgac cggctcgacg acgtcaagcc
    13741 gctttcatcg gccgagcggc gcgcaattca aagctttctg gagtccggtg tcgacctcga
    13801 cgacgtgctg cggcgcgcgg tacggctgct ggcacaactg acccgccagg tggccgtggt
    13861 tcagtacccg acgttatcga cgtcgacggt tcgccacctg gaagtggtcg cgctgtcacc
    13921 ggcccggttg ctgatggtgg tcatcacgga ctcaggccgg gtggaccagc gcatcgtcga
    13981 actcggtgat gtgatcgacg accaccagct ttcccagctg cgtgagatgc tcggccaggc
    14041 gctggtcggc aagaggctgt cggtcgcgtc gaccgcggtg gcagacttgg ccggtgatct
    14101 cgtcgggcgc agcgggctgg ccagcaacct gagcaacgcg gtgggccgct ccgcgacggt
    14161 gttgctggag tcgctgctag agcacactga agagcgcctg ctgatggggg gcgccgccaa
    14221 cctgacccga aacgccgcgg atttcggcgg ttcgctgcgg tccatcctgg aggccctcga
    14281 ggagcaggtg gtggtgctgc gattgctggc ggcccagcag caagccggca aagtgacggt
    14341 gcgcatcggc cacgagacgg ccgtcgagca gatggtgggc acctcgatgg tgtccaccgc
    14401 ctacggcagc tcggataccg tgtacggcgg tatgggtgtg ctggggccca cccggatgga
    14461 ctatccggga actatcgcca gcgtcgccgc ggttgctctt tatattggcg aagttctggg
    14521 tgctcgatga acgcgcgccc gctgtgggat ttgtgaacag ccgcttagat gcggcttgag
    14581 aagctctatt aggagagtcg gcgtggcacg cgattattac gggctgctgg gcgttggtag
    14641 aaacgccagt gatgcggata tcaaacgcgc gtaccgcaag ctggcgcgcg aattgcatcc
    14701 cgacgtcaat cccgatgagg ttgcgcaggc gaagttcaag gaaatcagcg tcgcctacga
    14761 ggtgctgagc gatcccgaga aacgccggat cgtcgacctg ggcggcgatc cgatggaaaa
    14821 cgccgcgtcg gccggcggtt tcggtggttt cggtggcttg ggcgatgtgt tcgaggcttt
    14881 cttcggcggc ggcttcagcg gaggtgcggc ctcgcgcggt cctatcggcc gggttcggcc
    14941 ggggtcggat tcgctgctgc ggatgcggct ggatctcgaa gagtgcgcca gcggtgtgac
    15001 caagcaggtc accgtcgaca ccgcggtgtt gtgcgatcgg tgccagggca agggcaccaa
    15061 cgccgattcc gtgcccgtcc cctgcgacac ctgcggtggt cgcggggaga ttcagacggt
    15121 gcagcgttcg ttgctgggcc agatggtgac gtcacggccg tgtcccacct gccgcggcgt
    15181 cggggtggtc atccccgacc cgtgtcacca atgcgtgggc gacgggcggg tgcgcgcccg
    15241 ccgcgagatc agcgtcaaga ttcccgccgg tgtcggcgat ggcatgcgcg ttcggctggc
    15301 cgcccaaggc gaggtcgggc ccgggggagg gcccgcgggt gacctgtatg tcgaggtcca
    15361 tgagcaggcc catgacatct tcgtgcgcga gggcgacgac ctgcactgca cggtttcggt
    15421 gccgatggtc gacgcggcgc tgggcgccac cgtcagcgtc gaggccatcc tggacggaac
    15481 caccgagatc accatcgcac ctggcacgca gcccggttcg gtcatcacgc tgcggggtca
    15541 ggggatgccg cacctgcggt cgagcgcacg gggcaatctg catgcccatg tcgaggtggt
    15601 ggtgcccacc cggctggacc accatgacac cgaactgctg cgtgagctga agaatcgccg
    15661 cggccgcgat gtgcccgaag tccgttcgac ccacgccggc ggcgggctgt tcagccggct
    15721 ccgcgagacc ttcaccgggc gctaatcggg agcgttgagg gcccgcgcag atggtggcga
    15781 cgctgttcta cgttgacgca ctgcccgata ccggtgccct ggcggtcgtc gacggcgacg
    15841 aagggtttca cgccgccacc gtgcggcgga tccgacccgg cgaggaactg gtgctcggcg
    15901 acggcgctgg aggcctcgcc cgctgtcagg tcgagcgcgc gggccgcgat ggcctgcacg
    15961 cgcgggtgct gggacgctgg agcgtcgcgc ccgggcggcc ggcggtgacc gtggtgcagg
    16021 cgctgcccaa gtccgagcgt tcggagttgg cgatcgaact ggcgaccgaa gccggtgccg
    16081 acgccttcct cgcctggcag gccgcccgct gcgtggccac ctgggaaggt gcccgagtcg
    16141 acaagggtct gcgccggtgg cgtgccgtcg cccgttcggc ggcccggcaa tcccgccgcg
    16201 cgcacatccc gccggtcgag ggcgtgctgt ccaccgcggg cctgatccag cgggtgcgtg
    16261 acgaggtagc ggccggcgcc gcggtcctgg tcttgcacga gtcggcgacc gatgggcttg
    16321 cggatgtctt tccggacatc gccggcacgc aagcaaactc gctgttgctt ctggtcggtc
    16381 ccgaaggcgg tatcgggccc gatgaaatcg cggcgttaac cgaggcgggc gcagtggcgg
    16441 tgcggcttgg tccgcaggtg ttacggacgt cgaccgctgc cgcggtggcg ttgggcgcgc
    16501 tgggtgtgct gaccccgcga tgggacgtgc cggtggagag tcgcaacacc gaaagtgaac
    16561 ccaacgacgg gatgcatcgg tgattccctc gtcgaccgtt gggtcaccgt cccggccggc
    16621 ggtagactgg ggcagccgag cagacccctg acaagccgag aaagcaggca tcggaaacca
    16681 cgtgacgccc cgcgagatca gcgctgccgc cgcatccgga gccctccagg ccgacgctca
    16741 agttcgcagc agcatcgacg ttccgcccga tctcgtggtg ggactgctgg gttcggcaga
    16801 cgagaacctg cgcgccctgg aacgcacgct gaacgccgac ctgcatgtgc ggggcaatgc
    16861 cgtcaccctc tctggtgagc cggccgacgt ggcgctggcg gagcgggtga tctcggagct
    16921 ggtcgcgatt gtggccggcg gccagccgtt gacgccagag gtggtgcgcc acagcgtcgc
    16981 catgctggtt ggcaccggca acgagtctcc ggccgaggtg ctcaccctgg acatcttgtc
    17041 gcgccgggga aacacgatcc ggcccaagac cttgaaccag aagcgctacg tcgacgccat
    17101 cgacgccaac accatcgtgt tcggtgtagg ccccgcgggc accgggaaga cgtatctggc
    17161 gatggccaag gccgtccgcg cgctgcagac caagcaggta agccggatca tcctgacccg
    17221 accggcggtg gaagccggtg agcgccttgg atttctgccg ggcacactga gcgagaagat
    17281 cgacccgtac ctgcggccgc tgtatgacgc gctgtacgac atgatggatg ccgagctcct
    17341 cccgaagctg atgtccgccg gggtcatcga ggtcgcaccg ctggcataca tgcggggccg
    17401 gaccttgaat gacgccttca tcgtgctcga cgaggcgcaa aacaccaccg ccgagcagat
    17461 gaagatgttc ctcacccggc tcggtttcgg atccaagatc gtcgtcaccg gggatatgac
    17521 ccagatcgac ctccccggcg gtgcccgatc cggcctgcgc tcggcgatcg acatcctcga
    17581 gaatgtcgag gacatccaca tcgcggagct gaccagcgtg gatgtggtgc gccaccggct
    17641 ggtctcggag atcgtcgacg cctatgccag gtacgaggag cccggttcgg ggatgaaccg
    17701 ggccgctcgg cgagcgtccg gtgtccgcaa tcgccgatga tgggtgcggt gaacattctt
    17761 atgagcgcat ctgtgagcat ccgtgagtag ctatgagcat ctatgagcat tgaggtatcc
    17821 aacgagtcgg gcatcgacgt ctccgaagcc gaactggtca gcgtcgcgcg atttgtgatc
    17881 gccagcatgg acgtcaaccc cgccgccgag ctgtcgatgg tgttgttgga taccgcggcg
    17941 atggccgacc tgcacatgcg ctggatggac ctgcccggac cgaccgacgt gatgagcttt
    18001 ccgatggacg agctcgagcc gggcggccgc cccgacgccc ccgagccggg gccgtcgatg
    18061 ttgggcgaca tcgtgctgtg cccggaattc gctgccgagc aggcggccgc ggcgggtcac
    18121 agcctcggac acgagttggc gctgctgacg attcacggtg tgctgcatct gctcggctac
    18181 gaccatgcgg agccggacga ggaaaaagag atgttcggcc tgcaggagcg gctgctcgag
    18241 gagtgggtcg ccgaacaggt cgaggcctac cacaacgtcc tccaggagga gcgggaccgc
    18301 cggttgctgg acaagtcaag gtatttcgac aattgaccgg cctgtcccag ttgttcggcg
    18361 cgatcgcttt ggttgctctg ggcggccttt tcgcggcgat cgacgccgcc atcagcacgg
    18421 tgtcgctggc acgggtgcag gaactggtcc gcgacgggcg ccccggcgcg gtgtcgctgt
    18481 gcaaggtcgt gaccgagcgg ccgcgctaca tcaacctgat ggtgttgctg cggctgacct
    18541 gtgagatcac cgcgaccgta ttggtggtgg tgttcctcta cgacaacttc ggcatgagct
    18601 gggcgttgtt cggcaccgcg accaccatgg tggtggtcag cttcgtcgtg atcggagtgg
    18661 gcccacgcac cctcggccgc cagcacgcgt atcccatcgc gttggcaacg gcgttgccgc
    18721 tgcgggcgat ctcgtggctt ttgatgccgc tcagccgatt gctggtggtc ctcggaaacg
    18781 cggtcacacc ggggcggggc ttgcggaacg ggccgttcgc atccgagatc gaactgcgcg
    18841 aagtcgtcga tttggcccag cagcgcggcg tggtcgccgc cgacgagcgc cggatgatcc
    18901 agtcggtctt cgaactcgga gacaccccgg cccgcgaggt gatggtgccg cgcaccgaga
    18961 tgatctggat cgaaagcgac aagttggccg ttcaggcaac gaacttggcg gtacgcagcg
    19021 ggcattcccg cattccggtg atcggcgaaa acgtcgacga cgtcgtcggg gtcgtgtacc
    19081 tgaaagacct tgtccagcag acgttcttgt cggccgatgg cggccaggac accacggtgt
    19141 cgcacgtgat gcgcccggcg gtgttcgtgc ccgactccaa gccgctggac gcgctgctgc
    19201 gtgagatgca gcacgaccga aaccacatgg cgctgctcgt cgacgaatac ggcgcgatcg
    19261 ccggactggt cagcatcgaa gacgtgctgg aagagatcgt cggcgagatc gccgacgagt
    19321 acgaccaggc ggagacggcg cccatagaag acttgggcga caaatgtttt cgagtttcga
    19381 cgcggctgcc gatcgaagac gtcggcgaac tgtacggcgt ggaattcgac gacgatctcg
    19441 acgtcgacac cgtcggcggc ctgctggccc tggaattggg ccgcgttccg ttgccggggt
    19501 ccgaggtggt gtcacatggc ttgcggttgc gcgccgaggg cggcaccgat gatcgggggc
    19561 ggcttcgggt caccaccgtc ctgttgagcc cggccgaatc cgacagtctc gatgggccga
    19621 atggtgggga gcccaatcaa catgactgag cagttggccg ccgaggacgc caagttggtg
    19681 gtgctggcgc gggccgcgat ggcgcgcgcc gaagccgaca gcgccgccgc ggtgcgggat
    19741 gtcgacggcc gcacgtatgc ggctgcgccg gtggacctgc cggcgttgaa gttgaccgga
    19801 ttgcaggcgg cggtggccgc ggccgcatcc agtggtgcga ccgggctggc cgccgccgtc
    19861 ctggtggcag catccgccga tgaccccggg atcgaggcgg ttcgcaaact gtcgccgacg
    19921 gccgtcatca tcgtcaccga ccgggccggt gtcgtgctgt gagcatgacc gaattccgtt
    19981 cgggcttcgt atgtctggtc ggccgtccca ataccggtaa gtcgacgctg accaacgcgc
    20041 tggtcggcac caaggtggcg atcacctcga cgcggccgca gacgacgcgg cataccattc
    20101 gcgggatcgt gcaccgggag aacttccaga tgatcctggt cgacaccccg ggcctgcacc
    20161 ggccgcgcac cctgctgggc cagcggctga acgacctggt ccgcgacacc tacgccgagg
    20221 tcgacgtcat cgggctgtgc atcccggccg atgaggcgct cggtccgggc gaccggtgga
    20281 tcgtcgacca gatccgttcg gtcgcgcgca ccgcgaccgt ccagaagctc gtcgtcatcg
    20341 tcaccaagat cgacaaggtg cccaaggatc gcgtcgccgc gcagctgatg gcggtcagtg
    20401 agctggtgaa cgattcggcc gagatcgtgc cgttgtcggc ggtgaccggc gagcagctcg
    20461 aggtgttgat cgacgtgctc gccgcggcgt tgccccccgg acccgcctat taccccgacg
    20521 gtgagctgac cgacgaaccc gaagaggtcc tgatggcgga gctcatccgc gaggccgcgc
    20581 tggagggggt ccgcgacgag ctgccccatt cgctggcggt ggtgatcgac gaggtttccc
    20641 cgcgggaggg gcgcgacgac ctgatcgacg tgcacgcgct gctgtacgtc gagcgggaca
    20701 gccagaaggg catcgtgatc ggcaagggtg gcgcccggct gagggaagtg ggtaccgccg
    20761 cgcgcgccca gatcgagaag ctgctgggca ccaagatcta tctcgacctg cgcgtcaagg
    20821 tcgccaagaa ctggcagagc gaccccaaac agctcggtcg gcttggcttt tgacgctgcg
    20881 ggctcgcggc gctgccgcgc ggtcccggca gtcaccttct cgagggcgcc gagctgggcg
    20941 cgggaggcac gtctgtgccc gcgttccacc agatcgcggg ctgcctggtc gaccacccgc
    21001 agacggcctc cagctccgcc gccaacgaga tcaggaggcc ctcgctgttt gccggcccca
    21061 tcagctgcac accgatcggc aaaccttcgg aagtgaagcc cgccggcacg ttgatggatg
    21121 gccaccccag gacgttccac ggccacgtca acgggcacgc ggcgatcatg gcgcggtcgg
    21181 tgccgaggcc gcccaaccgg tcgaacgcgc gcgccaaggg cggcggctgg gcggtggtcg
    21241 gtgccaccac cacgtcgacg atgtcgaaga tcgagcccac ccggcgctgg gaggcggctt
    21301 cgtgccggcg cgcgctgcgc agaaccgctt gtcccagcac atggcccatc cgcaggttgg
    21361 acaaggtgcg gggatccagg atgacgccgt cacccagccg ctcctcccag tcccgcaggc
    21421 ccgaggtgga ccgggcgaga aagtcccacg acaatcgcag gccgtaatcg gggttgccgg
    21481 gcaccacggt gtggccgagt agctcgagct gcctgcgcac ggcccgcgtc gcggtcagga
    21541 tgtccgggtg caacttggcc cgaaagccgg tgtaggggaa tcgtgttgac agcgcgatgt
    21601 tcagcgggcc gggcgcgatt ccgacatagt cggatgccgt gatcgggggt ggtttgtgcc
    21661 ggtcaccctc gacgtttccg gatgcggcgt cgagcaccag cgcggcgtcg gcgatcgtgc
    21721 gggccagcac gccattgacc gtgatgccgt tgaatgcctc cggcaacggc caggtggaga
    21781 tgcggccacg ttgcggcttg atacccacca ggtgcgtcca cgcggcgggg atgcggacgc
    21841 tgcctgcgcc gtcggagccg atcgcggcgg tgaccaggcc cgcggcgacc gcggccgcgc
    21901 tgccgcccga cgatccgccc ggggtgtgcc ggcgcgacca gggattacgg gtgtgaccga
    21961 atccgggtcc gctggtaaac ggccattggc ccagttcgca ggtgttggtc ttgccgacga
    22021 tcaccgcgcc ggccgctttg aggcggcgca ccacctcggc gtcgtgggcg gcgggtgcga
    22081 catatccctc ggtgccgaaa gccgttggca ccccggcaac gtcgacgtcg tccttgaccg
    22141 cgatcggaat gcccagcagc ggggccgtgt
//
LOCUS       DASSGQ010000386        28659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_789315,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000386 DASSGQ010000000
VERSION     DASSGQ010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 28659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28659
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789315"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VFI55_16960"
     CDS             <1..479
                     /locus_tag="VFI55_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7075752.1"
                     /translation="RLLENAIARMLRGDPGVRLRSIAMRPDCQLDVAGLWGVLRINRY
                     TRMNGTARITDIGDYLRIPFEVLEPTFSSLVAAGYAQRDGEAMYLTPAGAQQVDYVYS
                     LLLAWFVDKLAGSPGFEGQPDRDEVRAALERIAHRVLAQRDWQDDELTAVTDSAGP"
     gene            515..1009
                     /locus_tag="VFI55_16965"
     CDS             515..1009
                     /locus_tag="VFI55_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7075753.1"
                     /translation="MSRIGTFADDDVYGWATKSPELGGALANFSRVVYTKNRLPLRTR
                     ELARAVIAHSNECVVCVNTRDADGPAAGVDEELYYHATEWRTWTGYSEQERLAAEFAH
                     RFATEHTALRDDEDFWSRCNEQFSEELLADLALSCALWVGMGRVLRTLDIGQACKLTL
                     PSRA"
     gene            1089..2441
                     /locus_tag="VFI55_16970"
     CDS             1089..2441
                     /locus_tag="VFI55_16970"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HET7075754.1"
                     /translation="MTATPLAAAAIAQLEAEGVDTVIGTVVNPAGLTQAKTVPIRRTN
                     TFADPGLGASPSWHAFAIDQSGIAFTDDVGVVGDQRLRIDLSALRIVGDGLAWAPASF
                     FEQDGTPVAACGRGTLGRVEAALKGAGIEAAIGHEIEFLLVDPHGGRLPSALWAQYGL
                     AGVLEHEAFVRDVNASATAAGVGIEQFHPEYGANQFEISLSPLPPVAAADQLALTRLV
                     IGRAARRHGLRVSLSPVPFAGSVGSGAHQHFSLVTPEGPVFSDGTGAAGMTPAGESAV
                     AGVISGLPEAQGILCGSIVSGLRMQPGNWSGAYACWGIENREAAVRFVKGGPGSPRGG
                     NVEVKIVDPSANPYLASAAILGLALDGIKRRAALPPETTVDPAKLSDSDRQRAGIVRL
                     PDDQVEVITALDNSTLLRSILGDPVVDMVVAVRRLEQERYADLGPEQLADKFRMAWSL
                     "
     gene            2438..3550
                     /locus_tag="VFI55_16975"
     CDS             2438..3550
                     /locus_tag="VFI55_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7075755.1"
                     /translation="MSVPGLSALAQHIADVALIDQHVHGCWLTAGDRIRFENALNEAN
                     TEPLADSGFDSQLGFAVRAQCGPVIGLPKHADPQAYWERRGQFSETELAGLFLPTAGV
                     SDWLVDTGIGGDVAGVLQLSALSGNRAHEIVRLEQVAEQAARAPGDYASAFAEIMHRR
                     AASAVASKSILAYRGGFDGDLTEPSASQVAEAAGRWRDRGGIRLQDRVLLRFGLHQAL
                     RLGKPLQFHVGFGDRDCDLHHANPLLLLDFLRQSGGTPIVLLHCYPFEREAGYLAQAF
                     NNVYVDGGLSVNHLGARAPAFIARLLELAPFGKLLYSSDGFGPAELHFLGAALWRNGI
                     HRVLREFVADGDWSESDAIRVVDLIAHDNAARVYRV"
     gene            3734..4024
                     /locus_tag="VFI55_16980"
     CDS             3734..4024
                     /locus_tag="VFI55_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075756.1"
                     /translation="MNTPSPGPGWWLASDGQWYPQRWENTFIYNTNESLPALLDELSS
                     LAKTYGEQGWEIVNSSVQRTQVAHHFKGYDKEGDLYFEWSIVCSMKRPLRPA"
     gene            4209..6488
                     /locus_tag="VFI55_16985"
     CDS             4209..6488
                     /locus_tag="VFI55_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET7075757.1"
                     /translation="MLHAIARLALATPRRIIGIALLVMVVSAIVGIPVTKNLSPGGFR
                     DQTAESWRAAQVLSDKFGQGDMRLIVAVTSDAGVHGEAADVAAAQLVAYLASFPFVTG
                     VKSAWTMPPAAAQQLISRDGKTGLVVAGITGGESGAQKHAKTITDRLPALTGVTVTVG
                     GDAVGNLQMIDQTKKDLLVMEAISLPLCFLVLVWVFGSMFAAALPLAVGVFAILGAMA
                     VLRAVALATDVSVFALNLILAIGLALAVDYTLLIITRFRDELADGAPRDAALRSTLAT
                     AGRTVLFSAMTVALSMAAMALLPIYFLKSFAVAGVAVIAFTAIASVTITPAAIVLLGA
                     RLDSPDVRTAARRLLRRPEPVQRRIEETFWYRGAKAAMRRAIPVGLAIIALLLILGAP
                     FLGAKWGYLDDRVLPSSTSARQVGDELRRDFATNSLTDVIVVLPNVVGITNPELDRYA
                     SELSLVPDVSAVSSPDGAFSGGRPVGPPAADAGSTDGSAFLTVSSAAPLYSPASEAQL
                     DRLHAVATPGGRDVQLTGWAQVNRDSARAVTRPLPLVLSLIGAITFLLLFMLTGSVVI
                     PLKALLLNLLSLTATFGALVWIFQDGHLGGFGTTATGTLVASVPVLLFCLAFGLSMDY
                     EVFLISRISEFWQASAKTRADNDESVARGLARTGRVVTAAALLMAVSFAPLMAANVSV
                     TRIFGVGIPLAVLMDATLVRMLLMPAFMRVLGRANWWAPAPLARLHQRFGISESGPRQ
                     TSPVASKAQLVSAVARSSK"
     gene            complement(6425..6895)
                     /locus_tag="VFI55_16990"
     CDS             complement(6425..6895)
                     /locus_tag="VFI55_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7075758.1"
                     /translation="MTEVSTIEVRGVVIGLWQALSRRDWDAVKTHLAEDCLYVDMPLP
                     VAAARGPEDILNRLKIGLEPLAGYENHDGVLVVDGPDAMYEHSETWTFPTGERGVLKF
                     VTVHKVALRNGEAKVIVWKDYWDMNSLVGFAPPNYFEDLATADTSWAFDATGLV"
     gene            6961..7881
                     /locus_tag="VFI55_16995"
     CDS             6961..7881
                     /locus_tag="VFI55_16995"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET7075759.1"
                     /translation="MTPLQRYIAEEIATDHADGLLSRREVMRRLALLGVGTAAASALI
                     AACSHQKPPAPPSASSSEMITTPPGSTQPPGMADALPTEPISWPGPQGELHAAWAQAA
                     SPRGGVLVVHENKGLNDWVRSVAGRLAGAGYSALAIDLLSEEGGTDSFRDPAQATAAL
                     GKVPPERFTHDLNSGVAQLRVRVPGTKVAVVGFCFGGGLVWQLLTSGALAVSAAVPFY
                     GPLPDNPDFAGSKAAVLAIYAALDSRVTGSQAAAKAALDAAGLVNEIVVEPNADHAFF
                     NDTGPRYNAAAAADAWQRLLDWFARYLTAP"
     gene            7898..8248
                     /locus_tag="VFI55_17000"
     CDS             7898..8248
                     /locus_tag="VFI55_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075760.1"
                     /translation="MLTRRQKILIAIALVAILAGGCGALVRGFWGVSQDCHDWVKGNG
                     YQLVHNDWWAKSRGCVARTPAGDELYHDEGLGDKAIGWAWQCGIFAAGTLPAVVIVAV
                     IAFRGRRNPRAQDP"
     gene            complement(8286..9644)
                     /locus_tag="VFI55_17005"
     CDS             complement(8286..9644)
                     /locus_tag="VFI55_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HET7075761.1"
                     /translation="MSAYERMRAAHLNAVQAGLEDHVTRLDWSRQRIERYQSWRLRSL
                     LAFARERSPFHARRLRGLDPARASVADLASLPMMTKQDARERWDAIVTVPDLDRDRAE
                     RILAEQQWFSYTAADQQIFSSGGSSGVRGVYVWDWQFFVSAACLAWRMQAREELRGTK
                     VSPPARLAVLQAGVPPHASTPLFDMPTTPGMETVVIAAGAPFDEVLASVAIARPTHLV
                     GYPSVIGRLARAALAGELRIAPVRVSTNSEPLLDEDQRAIHEAWHAPIHNLWGSTEIG
                     VQAVGCGRDQGLHICEDEVVLERVDDNGKPVGPGEPAARTLATGLANRTFPFIRYDLG
                     DQVTLLPGQCACGSAFTRIADIGGRRDDDFCYGATTVPAIAFRHVLGTDPRIAEYQVT
                     QTPAGADIVVVGNPNREVLISSLVAALRRFGLAEPTIRIHVADSLRRHQASGKLRRFV
                     PL"
     gene            complement(9663..11021)
                     /locus_tag="VFI55_17010"
     CDS             complement(9663..11021)
                     /locus_tag="VFI55_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7075762.1"
                     /translation="MKERLHWLAMHGFVRGVAAIGVRRGDLQARLIADPTLAADPVPF
                     YDELRALGPLVKGRVNYLTADHALAHELLRSDDFRVVNMGSNLPAPLRWLERRTRDDL
                     LHPLRAPSLLAVEPPDHTRYRKTVSAVFTPRAVAALRDRVEQTAGNLLDQLANPSSVS
                     GVSSVSSASDVVDIVRRYCSQLPVAIISDILGVPEQDRRRVLEFGELAAPSLDIGLPW
                     RQYRSVQRGIAGFNYWLAGHLRELRRNPSDNLMSQLIQTAESGSAETHLDQAELQGIA
                     GLVLAAGFETTVNLLGNGIRMLLDAPWHLDTLRQRPELWLNAVEEILRLDSPVQLTAR
                     VALDDVTVAGMRIQRGELVVVYLAAANRDPSVFPDPHRFDIERPNAGRHLAFSGGRHF
                     CLGAALARAEGEVGLRTFFDRFPEARAAGTGSRRDTRVLRGWSALPVALGPARSMAPA
                     DR"
     gene            complement(11078..11491)
                     /locus_tag="VFI55_17015"
     CDS             complement(11078..11491)
                     /locus_tag="VFI55_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003899062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LpqH"
                     /protein_id="HET7075763.1"
                     /translation="MQNRLLAVAFVVVAGAAGCAQAETTPRKGAQLSIGGVSRTTRPP
                     ACSQLQSYRTIDLRDQNGQVEAVVLLSGYRAIPQWVKIRNIDGFTGSFWQGGVGDAHA
                     DVTNTAYTITGTAYGINSSDPNKAVTTDFKITAEC"
     gene            complement(11503..12336)
                     /locus_tag="VFI55_17020"
     CDS             complement(11503..12336)
                     /locus_tag="VFI55_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7075764.1"
                     /translation="MKSVFITGAGSGMGREGAKLFHAKGWRVGAVDRNDDGLATLKQE
                     LADGRLWIRAVDVTEKAALEGALADFCDGNAGGGLDMMWNNAGIGESGWFEDVPYDAA
                     MRVVEVNFKAVLTGAYAALPYLKKTPDSLMFSTSSSSGTYGMPRIAVYSATKHAVKGL
                     TEALSVEWQRHGVRVADVLPGLIDTAILTNTTNHSDDAGATMTAEELRASAPTKGMFR
                     LMPATSVAEAAWQAYQNPKRLHWYVPESIRFIDLLKGLSPEFVRGRIVRSLPGLMPKR
                     Q"
     gene            complement(12377..12850)
                     /locus_tag="VFI55_17025"
     CDS             complement(12377..12850)
                     /locus_tag="VFI55_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7075765.1"
                     /translation="MCLDQAMEGSATVHMAAPAGKIWDLIADVRNTARFSPEVFEAEW
                     LGDATGPELGAKFRGHVRRNEIGSVYWTTCEVTACEPGREFGFAVLLGDRPVNNWHYR
                     LAPSGGGTDVTESFRLNPAPWLGVYWLFGGFLRKRRNIRDMTKTLTRIKNVVEAG"
     gene            complement(12934..13347)
                     /locus_tag="VFI55_17030"
     CDS             complement(12934..13347)
                     /locus_tag="VFI55_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015627732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HET7075766.1"
                     /translation="MRKLCKPLVKSAMVGGIVAVSLSSSAGVANAAPAAPNWDAIAQC
                     ESGGNWQANTGNGEYGGLQFKPGTWAQYGGVGNPAAASRDQQIAVANRVFAEDGLDPW
                     PKCGSASGLPIGLYSHPAQGIKQIINGLIQAAMPH"
     gene            complement(13478..14047)
                     /locus_tag="VFI55_17035"
     CDS             complement(13478..14047)
                     /locus_tag="VFI55_17035"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET7075767.1"
                     /translation="MTDTHRHAARVAWSGLAVLLAVLPAAQARADPAGALTDLVDAAA
                     QRLEVAEPVAAFKWRSQAPIEDPARVQEELRALSSANSPDPGYVTRVFGDQINATEAI
                     EHSRFAEWKLNPAAVPGSPPDLSASRSTIDGLNQTMLTQIAADWELLHAPACPTQLDV
                     ARSDVIRDRQLDGLYQRALLLATQSYCQG"
     gene            14136..14753
                     /locus_tag="VFI55_17040"
     CDS             14136..14753
                     /locus_tag="VFI55_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075768.1"
                     /translation="MPWTVDMRRPGEVGGKRAEWLPAWQVRALTAAALTISLLLGGAL
                     VLVDRLHSTTSDVLDHPADPVTDDQSEAQVVESTKQIVTLTGLRTTSAGYTLMSCKDR
                     QDPPYQGAIYLNFALPSATRPDTYFPTIAATLVSHGWTEGLPPNDHPFARIFSKDAVT
                     VTVYRHSDDPGLGVLRAYGQCRNINDHHKDATTWTDVTAQFTSSA"
     gene            complement(14827..15819)
                     /gene="ag85B"
                     /locus_tag="VFI55_17045"
                     /gene_synonym="fbpB"
     CDS             complement(14827..15819)
                     /gene="ag85B"
                     /locus_tag="VFI55_17045"
                     /gene_synonym="fbpB"
                     /EC_number="2.3.1.122"
                     /EC_number="2.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol
                     acyltransferase/mycolyltransferase Ag85B"
                     /protein_id="HET7075769.1"
                     /translation="MTEASEKIRAWGRRLLVGAAAAVTLPGLIGLAGGAATAGAFSRP
                     GLPVEYLQVPSGAMGRDIKIQFQSGGDGSPAVYLLDGLRAQDDYNGWDINTPAFEWYY
                     KSGLSVIMPVGGQSSFYSDWYNPACGKAGCTTYKWETFLTSELPGWLSANRSVKSTGS
                     AAIGISMAGSSAMILAAYHPGQFIYAGSLSALMDPSQGMGPSLIGLAMGDAGGYKAND
                     MWGPSSDPAWQRNDPSLQIPKLVGNNTRLWIYCGNGTPSELGGANMPAEFLENFVRSS
                     NLKFQDAYNAAGGHNAVFNFNANGTHSWEYWGAQLNAMKPDLQGTLGATPGGGG"
     gene            16546..17658
                     /locus_tag="VFI55_17050"
     CDS             16546..17658
                     /locus_tag="VFI55_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016706292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075770.1"
                     /translation="MDAVLGLSVTPTTVGWVLAEGHSADGTILDHRELPLHAGDGVAA
                     VSTAEQVADEVLRVNELAAASDHHLRVIGLTWNDEASAQAALLVEALTDAGFDNVVPV
                     RLIEAVETLARAIAPVIGYEQTAVCVVEPEWATVVMVDTHNGETQTVVKHVRGGFDGL
                     TRWLSGMFDQDGWRPGGVVAVGADSEINGFSWQLEKALPIPVFAQTMAQVTIARGAAL
                     AAAASTDFTDELLVAHIGEPAVAPARPRKLSHAGALTALAAGAITFVTSLSLAVGIQL
                     APTSEPAQKHVVHEPTPQIAEAVAPPAEAAPPAPQSKAPAGAPAQEAGAPGDQDGAPA
                     DQQSDPNARQPYLTRVLEHIPGDAGDSAAPTQPQSP"
     gene            complement(17746..18303)
                     /locus_tag="VFI55_17055"
     CDS             complement(17746..18303)
                     /locus_tag="VFI55_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET7075771.1"
                     /translation="MTDAWTLDASDGELVIHTGVAGRAARMGHRLTIAMKRWQATVGW
                     AGAEPVTAELIVEIDSFEVLRGEGGVKALSGPEKVLVKSNAMKSLDAAHYPQIRFIAD
                     GAEQIAKIADGYRLTGMLHIRGRSREQVIDLRTEDLGTAWRMSVESAVRQTDYGIKPY
                     SLLMGSLQVADEVSLSFTAVHAKDG"
     gene            complement(18317..19330)
                     /locus_tag="VFI55_17060"
     CDS             complement(18317..19330)
                     /locus_tag="VFI55_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HET7075772.1"
                     /translation="MATHKAVQVSSAGAALELVDVETVPPGRGEVRLTVTACGICGTD
                     QHFVEGGFPGMSWPLTPGHEIAGAIAELGEGVEEFAVGDRVAVGWFGGCCFHCDPCRK
                     GSFIHCVNGKVPSWQYPGGYAESVTAPANALARIPSELSDVEAAPLGCAGVTTYNALR
                     HTKALPGDRVAILGVGGLGHLGVQFSRAMGFETIAIARGTAKAEDARELGAHHYIDST
                     SGDVAEAMRKLGGAAVVLATAGNSPAMAATVGGLLPQGELVTIGVTPEPLPISPVQLI
                     TPGLSIVGHPSGTSKDVEDTMHFAVLSGVRAWTQELPLAQAAEGYAAMEHGRAHYRTV
                     LTM"
     gene            complement(19355..20626)
                     /locus_tag="VFI55_17065"
     CDS             complement(19355..20626)
                     /locus_tag="VFI55_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7075773.1"
                     /translation="MPSPAIPNLPPGFDFTDPDIYAQRLPVEELSEMRRNAPIWWNEQ
                     PIGTGGFDDGGFWVVSKHKDVKEVSLRSDVFSSLQKTALPRYKEGTVREQIDTGKFVL
                     LNMDAPQHTRLRKIISRGFTPRAVERLRDDLNERARRIVEAAAAEGSGDFVEQVSCEL
                     PLQAIAGLLGVPQEERMKLFQWSNQMVGDQDPEFATNDGLTASIQLITYAMQMAADRA
                     NNPGPDIVTKLVEADIDGHKLSDDEFGFFVILLAVAGNETTRNSITQGMMAFTDFPDQ
                     WELYKRRRPVTTADEIVRWATPVTSFQRTALEDYELSGVRIKKGQRVVMVYRSANFDE
                     DVFDDPFSFNILRDPNPHVGFGGTGAHYCVGANLARMTIDLMFNAIADAMPDLEPVGK
                     PERLRSGWLNGIKHWQVDYHTDRAAKCPVAH"
     gene            complement(20656..20793)
                     /locus_tag="VFI55_17070"
     CDS             complement(20656..20793)
                     /locus_tag="VFI55_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075774.1"
                     /translation="MSVLQTGAMPETHRGCNTLQFGFGDLALVLPNRGVAMVYLAEQD
                     D"
     gene            20821..21417
                     /locus_tag="VFI55_17075"
     CDS             20821..21417
                     /locus_tag="VFI55_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET7075775.1"
                     /translation="MHIVLSDFMSLDGVVQSPGAQGEDTDGGFAHGGWSMRYFEPETM
                     GPAVTETMASTDALLFGRRTWQTMAAAWPTRAGDPFADRMNEMPKYVASRTLTRDDLT
                     WPGSTLLPANDATGAIGAIRELREQDGGAIQVMGSASLAAQLIEHDLVDEYRLMIEPV
                     LLGGGKRVFPDDGRARALELVSATTTGTGVLICTYRRV"
     gene            21546..21950
                     /locus_tag="VFI55_17080"
     CDS             21546..21950
                     /locus_tag="VFI55_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075776.1"
                     /translation="MIREFLAVAALAGGTVSVAPCAAADPHYDGDVPGMNYQALLGAP
                     CDNYERFIFGRGPSGQAEACHFPPPNQFPAATIGYWVISYPLYGVQQAGAKCPGSQAA
                     AQSAAGLPMLCLGNQGWQEGWFTGAGFFPPAG"
     gene            22042..22353
                     /locus_tag="VFI55_17085"
     CDS             22042..22353
                     /locus_tag="VFI55_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075777.1"
                     /translation="MHNYPSYPSVDRPAGSPIPRWLRFVLASDRAGSAWYIGTGFFFA
                     PALALLSPWPAVTAVLWGVIGLAGLWLGLLGVAMAVGLARILRSGAEIPEEYWRTLVN
                     Y"
     gene            22427..22648
                     /locus_tag="VFI55_17090"
     CDS             22427..22648
                     /locus_tag="VFI55_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075778.1"
                     /translation="MTFNPKDAVDAAKDIATNAVEKASDIVEHAGDIIRGDIKGGASG
                     IVQNSIDIATHAVDRAKEVFTGKGEADPE"
     gene            complement(22684..23814)
                     /locus_tag="VFI55_17095"
     CDS             complement(22684..23814)
                     /locus_tag="VFI55_17095"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HET7075779.1"
                     /translation="MHTAICDDLAIEFPIFAFTHCRDVVVAVSRAGGFGVLGAVGFTP
                     EQLEIELNWIDEHIGDHPYGVDIVIPNKYEGMDSHLSADELAETLRKMVPQQHLDFAK
                     KILADHGVPVENADEDTLQLLGWTEATATPQVEMALKHPKVAMIANALGTPPADMIKH
                     IHDSGRKVAALCGSPSQARKHADAGVDIIIAQGGEAGGHCGEVGSIVLWPQVVKEVAP
                     VPVLAAGGIGSGEQIAAALALGAQGAWTGSQWLMVEEASNTPVQQAAYAKASSRDTVR
                     SRSFTGKPARMLRNDWTEAWEQPDNPKPLGMPLQYMVSGMAVRATNKYPNETVDVAFN
                     PVGQVVGQFTKVEKTAAVIDRWVQEYLAATTRLDELNAAAAV"
     gene            complement(23948..24412)
                     /locus_tag="VFI55_17100"
     CDS             complement(23948..24412)
                     /locus_tag="VFI55_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075780.1"
                     /translation="MATLLAACGGFLLAVLWMDLIFDVQVLGHRDAGEELPEPVLASI
                     AGYYHRATTTSRPMSRLIVVVMVVLLGALAFQAARGNDPAWLLPISAALAGVPVALAL
                     THTVPNAIQLGHRADSAAEQTRLARSICRDHLICAGCMLTFLVLWIAYTATV"
     gene            24735..27374
                     /locus_tag="VFI55_17105"
     CDS             24735..27374
                     /locus_tag="VFI55_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020786886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7075781.1"
                     /translation="MTFLFTDVEGSTRRWEADADTMRVALAAHDEVLRQAIEAHGGWM
                     FKHTGDGVCAAFASPRSALDAAVAAQRALELPVRMGIATGEAELRGADYFGVVLNRAA
                     RVMAAGHGGQILVADSTAGLLSGADLVDLGARRLRDLPTAIGLFQVRAPGLRTEFPPP
                     RVLDSSVGNLRPQASSFIGRESELAEVQASLRGRRFETLTGVGGVGKTRLALELATRL
                     ADEFPDGVWVFELANVSDPAAVPDAVASVLGVSQQPGKTLADSVAIALEGRVRLLVFD
                     NCEHVLDAAADLIETILRHSTTVTILATSRERLGLAQEQLWPVPSLDVRAGIDSAAVT
                     LFVERARSVAPNFSLTRPDEASAVLEICRRLDGIPLAIELAASRMASMTAIEVRDRMD
                     QRFRLLVGSRRGLGRHQTLRDAVAWSYDYLDDAEKALLERCSVFAGGFDLDGACAVVG
                     PDGPDDLAVLDLLDALVRKSLLVADRSSARTRFSMLETIRQFADAQLVATGAATQARA
                     AHARYFAGREADVLALWDSPRQREAYTWFIVELANLRTAFRWAAEQGDLDVATTIATY
                     ATFLGLWVENYEPIAWAEESIEPALAVDHPRLAFLYTLASHCWYPGRLEEAIHYSDAG
                     QVVMRSDGRGVPFGFEGVLRNPYLAIGQPGRCAEWCRARLADGPDTHTFTRATLTIAL
                     TMAGAGEEARAAANGLIDAADATHNPYTISFALWAYGYTFRDAAPAEALDAMRRGLAI
                     AQDSGNRLTETRLTGTLSRLEAQYGDPLAALDYLTLVMRNYHDAGNTAMMRTPLAVLA
                     ALIDRLGRYEPAAAIAGFAASPLTAVAVPEFNTAIAHLRDALGGPAYESIARKGQQMT
                     TAAMAAYAYDQIDQARTGLTHPN"
     gene            27554..28579
                     /locus_tag="VFI55_17110"
     CDS             27554..28579
                     /locus_tag="VFI55_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7075782.1"
                     /translation="MRTVVVDGTRSIRVDIRPDPALPGADGAIVSVTAAGICGSDLHF
                     YEGEFPFAEPVALGHEAVGTVIETGSRVSTVEVGDRVMVSSVAGCGSCPGCATKDPVM
                     CYSGLQIFGSGALGGAQADLLAVPAADFQLLKIPEGINDEQALLLTDNLATGWAAAQR
                     ADTSFGSTVTVIGLGAVGLCALRSAFVQGAATVFAVDRVDGRLDRAARWGATPLKAPA
                     AEAIIAATRGRGADSVIDAVGTDASMTDALNAVRPGGTVSVVGVHNLQPFPLPALSCL
                     MRSITLRLTTAPVQRTWPELIPLLQSGRLDVDGIFTTTLPLDEAAKGYATAESRSGDV
                     VKILLRP"
BASE COUNT         4665 a         9578 c         9563 g         4853 t
ORIGIN      
        1 aacgcctgct ggagaacgcc attgcacgca tgctgcgggg cgatcccggg gtgcgactgc
       61 gcagcatcgc gatgcggccc gattgccagc tcgacgtcgc cgggctgtgg ggcgtcctgc
      121 gcatcaatcg ctacacccgg atgaacggga cggcccggat caccgacatc ggcgactact
      181 tgcggatacc tttcgaggtg ctggaaccca ccttctccag cctggtcgcc gccgggtacg
      241 cgcagcgcga cggcgaagcg atgtacctga ccccggccgg ggcccagcag gtcgattacg
      301 tgtactcgct gctgctggcc tggttcgtcg acaagctggc cggatcgccg ggcttcgagg
      361 gacaaccgga ccgcgatgag gtgcgggccg cgctcgaacg tatcgcgcat cgcgttctgg
      421 cgcaacgcga ttggcaggac gacgagctca ccgcggtcac cgactcggcc ggtccgtagc
      481 ccggcgatgt gcggggcggg cgtagcatcg cgccatgagc cgaatcggaa cattcgctga
      541 cgacgacgtc tacggctggg ccaccaaatc cccggagctc ggcggggcgc tcgccaactt
      601 cagccgcgtc gtgtacacca agaaccggct gccgctgcgc acccgtgagc tcgcgcgcgc
      661 ggtgatcgca cacagcaacg aatgcgtcgt gtgcgtcaac acccgggacg ccgacgggcc
      721 cgccgccggc gtggacgagg agctctacta ccacgcgacg gagtggcgca cctggaccgg
      781 ctacagcgaa caggagcggc tggccgccga gttcgcccac cgattcgcca ccgagcacac
      841 tgcgctgcgc gacgacgaag acttctggag caggtgcaac gagcagttct ccgaggagtt
      901 actcgccgac ctggccctgt cgtgcgcgtt gtgggtcggc atgggacggg tcttgcggac
      961 cctggacatc ggccaggcct gcaagctgac gttgcccagc cgcgcgtagg cgccggcgcg
     1021 cgacatccgc gcacttcagc acctgctcag tccctgcacc ggttgatccg cactgcagaa
     1081 tggctgccat gacagccacc ccgctcgccg ccgccgcgat cgcccagcta gaggccgaag
     1141 gcgtcgacac ggtcatcggc accgtcgtga accccgccgg gctgacgcaa gccaaaacgg
     1201 tccccatccg gcggaccaac acgttcgccg atcccggcct gggcgccagt ccctcgtggc
     1261 atgccttcgc gatcgaccag agcggcatcg cgttcaccga tgacgtcggt gtggtgggcg
     1321 atcagcgtct gcgcatcgac ctttcggcat tacggatcgt cggcgacggg ttagcctggg
     1381 cgcccgcctc gtttttcgag caggacggca cacccgtggc ggcgtgcggc cggggaacgc
     1441 tgggccgggt ggaggccgcg ctcaagggtg ccggcatcga ggcggcgatc ggtcacgaga
     1501 tcgagttcct tttggtcgac ccgcacggtg gccggctgcc ttcggcgctg tgggcgcagt
     1561 acggcctcgc cggcgtcctc gagcacgagg cgttcgtccg tgacgtcaac gcgtcggcca
     1621 ccgcggccgg cgtcggaatc gagcagttcc atcccgaata cggggccaac cagttcgaga
     1681 tctcgctgtc cccgttgccg ccggtggccg cggccgatca actggcattg acccgcctcg
     1741 tgatcggccg ggccgcccgc cggcacgggc tccgcgtcag cctgtcaccg gtgcccttcg
     1801 cgggcagcgt cggatcgggt gcacaccaac acttttcgct ggtcacgccg gaaggtccgg
     1861 tgttttccga cgggaccggc gcggcgggta tgacgccggc gggggagagc gcggtcgcgg
     1921 gggtcatcag cggcctaccc gaagcccagg gcatcctgtg cggatcgatc gtctcgggtt
     1981 tgcggatgca gcccggtaac tggtccgggg cctacgcgtg ctggggtatc gagaaccgtg
     2041 aggcggcagt gcgattcgtc aaaggcggtc cgggcagccc gcgcggcggc aacgtcgaag
     2101 tcaaaatcgt cgatccgtcg gcgaacccgt acctcgcgtc cgcggcgata ctcgggctgg
     2161 cgctggacgg gatcaagcgt cgggcggcac tgccacccga aaccaccgtc gacccggcaa
     2221 agctttccga ttcggaccgc cagcgcgccg gtatcgtgcg cctgcccgat gaccaggtgg
     2281 aagtcatcac cgcacttgac aattcgacac tgctacggag catcctgggc gatccggtgg
     2341 tcgacatggt agtcgcggtc cgccgcctgg agcaggagcg ctacgccgac ctcggccccg
     2401 agcagctggc cgataagttc cgcatggcct ggagcctttg agcgtgcccg gcttgtcggc
     2461 tctggcgcaa cacatcgccg acgtggcgtt gatcgaccag cacgtccacg gatgctggtt
     2521 gacggccggg gaccggatcc ggttcgagaa cgccctcaac gaggccaaca ccgaaccact
     2581 tgcagactcg ggtttcgatt cccagctcgg cttcgccgtg cgcgcgcagt gcggccccgt
     2641 catcggcttg cccaaacacg ctgacccgca ggcatattgg gagcgtcgcg gccaattcag
     2701 cgagaccgag ctggccggcc tgttcttgcc gaccgccggg gtgagcgact ggctggtgga
     2761 cacggggatc ggcggcgatg tcgcgggcgt cttgcagctg agcgcactgt ccggcaaccg
     2821 cgctcacgag atcgttcgtc tggaacaggt ggccgagcag gccgcgcggg cgccgggcga
     2881 ctacgcctcg gcgttcgcgg agatcatgca ccggcgcgcg gccagcgcgg tcgcctccaa
     2941 gtcgatcctg gcctaccgcg gcggattcga cggcgatctg accgagccgt cggcgtcgca
     3001 ggttgccgag gcggccggcc ggtggcgcga tcgcggtggt atccgattac aggaccgggt
     3061 gctgctgcgt ttcgggttgc atcaggcgct gcgccttggc aagccgctgc aattccacgt
     3121 cggcttcggc gaccgggact gcgatctgca ccatgccaac ccgcttctgc tgctcgactt
     3181 ccttcgacaa tccgggggca ccccgatcgt gctgttgcac tgctacccct ttgagcgcga
     3241 agccggatat ctggcgcagg cgttcaacaa cgtctacgtc gacggcgggt tgagcgtcaa
     3301 ccatctggga gctcgggcgc cggcattcat tgcccggctg ctggagctgg cgccgttcgg
     3361 caagctcctg tactcatcgg acggcttcgg tcccgcggaa ctgcactttc tcggtgcggc
     3421 cttgtggcgc aacggtattc accgtgtgct cagggagttt gtcgccgacg gcgactggag
     3481 cgagagcgac gccatccggg tggtcgacct gatcgcccac gacaacgccg cccgcgtcta
     3541 tcgcgtgtga cgcacggtgc ccggccggtg ccggggagcc tgggggacgt gtcgcagtgg
     3601 acccgcaaac aggttgtgtc gcgggttgtt aaacccgatc tcgaccagcg ggaccggcgg
     3661 gtcgccagta ttacctgaaa tgtcactgaa tgatcttttt ccagctgctc agggctccgt
     3721 aggatgccgg gacatgaaca ctccttcgcc cgggcctggt tggtggctgg cttcagacgg
     3781 acagtggtat ccgcaacggt gggagaacac cttcatctac aacacgaacg agtccttgcc
     3841 cgcgctgttg gacgagctga gctcactggc gaaaacctat ggggaacaag ggtgggagat
     3901 cgtcaactcc tcggtgcaac gtacccaggt cgcccaccac ttcaagggct atgacaaaga
     3961 gggtgacttg tatttcgaat ggagcatcgt ctgtagcatg aagcggcctc tgcggcctgc
     4021 gtaagcgttt gggcttcggc accgccggat ccgtcgcgac ggcgcgctga ctactcacga
     4081 caacaccggc gatgccgcct gattcaggta gtcggctacg cgtgtcagtg ccatccggtc
     4141 gcttctacgg tgcgttcatg gcagctccca ccgccaattc ctgaaccgcc gcgccggaag
     4201 gtccgtgaat gctgcacgca atcgcacgac tggccctcgc cacgccccgc cggatcatcg
     4261 gtatcgcgtt gctcgtcatg gtggtctcgg cgatcgtcgg catcccggtc accaagaacc
     4321 tgtccccagg cggtttccgg gatcagacgg ccgaatcatg gcgcgccgcg caggtactgt
     4381 ccgacaagtt cggtcaaggt gacatgcggc tgatcgtcgc ggtcacctcc gacgccggcg
     4441 tacacggcga ggctgcggac gtcgccgccg cccagctcgt cgcgtacctg gcgagcttcc
     4501 cgttcgtgac gggggtgaag tccgcatgga cgatgccccc ggcggcggcg cagcaattga
     4561 tcagccgcga cgggaagacc ggactggtcg tcgcgggcat caccggcggt gaaagtggtg
     4621 cccaaaagca cgccaagacc attaccgatc ggttgccggc cttgacgggc gtcacggtga
     4681 ccgtgggcgg cgacgcggtg ggcaacctgc agatgatcga ccagaccaag aaggacttgc
     4741 tggtgatgga agcgatctcg cttccgctgt gtttcctagt cctggtttgg gttttcggta
     4801 gcatgttcgc ggcggcgctg ccgttggcgg tcggtgtgtt cgcgattctg ggcgcgatgg
     4861 cggtcctgcg tgcggtggct ttggccaccg acgtatcggt cttcgcgttg aacctgatcc
     4921 tcgcgatcgg tctggcgttg gccgttgact acacgctgct gatcatcacc cgctttcgcg
     4981 acgaactcgc cgacggtgca ccgcgcgacg cggcactgcg gagcacgttg gccaccgccg
     5041 gccggacggt gctgttttcg gcgatgacgg ttgccctctc gatggccgcg atggcgctgt
     5101 tgccgatcta ctttttgaag tcgttcgcgg tggcgggcgt cgcggtgatc gcgttcaccg
     5161 ccattgcgtc ggtgacgatc accccggcgg ccatcgtgct gctcggcgcg cgccttgact
     5221 cgcccgacgt ccgcacagcc gcgcggcgcc tgctccgccg accggagccg gtacaacgca
     5281 ggatcgagga gaccttctgg taccgcggcg cgaaagccgc gatgagacgg gcgattccgg
     5341 tcgggctggc gatcatcgcg ctgctgctga tccttggcgc acctttcctc ggggccaaat
     5401 ggggttacct ggacgatcgc gtgttgccca gttcgacctc ggcccgtcag gtcggggacg
     5461 agctgcggcg cgatttcgcc accaactcct tgacggatgt gatcgtcgtg ctccccaacg
     5521 ttgtcgggat cactaatccg gagctggacc ggtatgcatc ggagctgtcg ttggtgccgg
     5581 atgtgtcggc ggtgtcgtcc cccgacggcg ccttttccgg aggccgtccg gtggggccgc
     5641 ccgcagcgga tgcgggctca accgatggca gcgccttcct caccgtcagt tcggccgcac
     5701 ccctgtactc gccggcgtcg gaggcccagc tggatcggct gcacgccgtc gcaactcccg
     5761 gcggccggga tgtccagctg acggggtggg cacaagtcaa tcgcgacagt gcgcgagcgg
     5821 tgaccaggcc tctgcccctt gtgctttcgt tgatcggggc gatcacgttc ctgctgctgt
     5881 tcatgctcac cggcagcgtg gtcatcccgc tcaaggcatt gctgctcaac ctgctgtcgt
     5941 tgaccgcgac attcggcgca ctggtgtgga tcttccagga tggtcatctc ggggggttcg
     6001 gcacgacggc caccgggaca ctggtcgcca gtgtgccagt attgttgttc tgcttggcat
     6061 ttggtctctc gatggattac gaggtgtttc tgatctcgcg aatctccgag ttttggcagg
     6121 catctgcaaa gacccgagcc gacaacgacg agagcgtcgc ccgtggcctg gcccgcacgg
     6181 ggcgcgtggt gaccgccgcg gcgctactga tggcggtgag tttcgccccg ttgatggccg
     6241 cgaatgtctc ggtcacacgg atattcgggg tcggaatacc gttggccgtg ctgatggacg
     6301 ccaccttggt ccggatgctg ttgatgccgg ccttcatgcg ggttctcggc cgcgcgaatt
     6361 ggtgggcgcc cgcaccgctc gcccggctac accagcgctt cggcatcagc gaatccggcc
     6421 cgcgtcaaac cagcccggtg gcgtcgaaag cccagctggt gtcggccgtt gcgaggtcct
     6481 cgaagtaatt cgggggcgcg aagccgacga ggctgttcat gtcccaatag tccttccaga
     6541 caatcacttt cgcctcacca ttgcgcaggg ccaccttgtg cacggtgacg aacttcagca
     6601 cgcctcgctc cccggtcggg aacgtccacg tttcggaatg ctcgtacatc gcgtcggggc
     6661 catcgaccac cagcacgcca tcgtgattct cgtaaccggc cagcggctcg agcccgatct
     6721 tgagccggtt caggatgtcc tccgggccgc gtgccgcggc caccggtagc ggcatgtcga
     6781 cgtacaggca gtcctcggcc aagtgcgtct tgaccgcgtc ccagtctcgt cgcgaaagtg
     6841 cctgccagag cccgatcacg acgccccgga cctcgatcgt ggaaacctct gtcatagcgg
     6901 ccaataaact cggtcaccga ataggtgtca accacagttt taccgcgcat actcgccagc
     6961 gtgaccccgt tgcagcggta catcgccgag gagatcgcga ccgaccacgc cgacggcctg
     7021 ctgagccgac gcgaagtcat gcgacgcctg gcgctgctcg gggtggggac ggcggccgcc
     7081 tccgcactga tcgcggcctg ttcccaccag aaaccaccgg cgccaccatc ggcgtcgtct
     7141 tccgaaatga tcaccacccc accgggttcg acgcaaccgc cgggaatggc cgatgcgctg
     7201 ccgacggagc cgatcagctg gccgggtccg cagggcgagc tgcacgccgc atgggcccaa
     7261 gcggccagtc cccggggcgg cgtgctggtc gtccacgaga acaagggatt gaacgactgg
     7321 gtacggtcgg tggcgggccg cctggccgga gccgggtact cggcgctggc catcgacctg
     7381 ttgtccgaag agggcgggac ggacagcttc cgggatccgg ctcaggcgac cgccgccctg
     7441 ggcaaggtcc cgccggagcg gttcacgcac gacctgaact cgggtgttgc gcagctgcgg
     7501 gtgcgtgttc cgggcaccaa ggtggccgtg gtcggcttct gcttcggcgg cggattggtg
     7561 tggcagctgt tgacctccgg cgcgctggcg gtttcggccg ccgtcccgtt ctacggtccg
     7621 ctgccggaca acccggactt cgccggatcc aaggccgcgg tgctggcgat ctacgccgcc
     7681 ctcgactccc gcgtcaccgg gtcccaggct gcggccaaag cggccctgga tgctgccggg
     7741 ctggtcaacg agatcgtcgt cgaacccaac gccgaccacg cgttcttcaa cgacaccgga
     7801 ccccgctaca acgccgccgc ggccgccgac gcgtggcaac ggctgctgga ttggttcgcg
     7861 cgttacctca ccgcgccgtg atcggcgggg cagttccgtg ctgacccgaa ggcagaagat
     7921 tctcatcgcc atcgccctcg tcgcaatctt ggccggcggc tgcggggcgc tcgttcgcgg
     7981 attctggggt gtcagccagg actgccacga ctgggtgaag ggcaacggct accagctcgt
     8041 gcacaacgac tggtgggcca agagccgcgg ctgcgtggcc cgaacacccg ccggcgacga
     8101 gctctaccac gacgaaggac tcggcgacaa agcgatcgga tgggcttggc aatgcgggat
     8161 tttcgccgcc gggacgttgc ccgcggtggt catcgtcgcg gtgatcgcgt tccggggccg
     8221 gagaaatccg cgcgcacagg acccgtagca gtcgagggag ccaccgaccc tgccggcccg
     8281 cggtgctaga ggggaacgaa tcgcctcagc ttcccgctgg cctggtgtcg ccgcaaactg
     8341 tcggccacgt ggatccggat cgtcggctcg gccagcccaa accgccgcag cgcggcgacc
     8401 agcgaggaaa tcagcacctc gcggttcggg tttcccacca ccacgatgtc ggctcccgcg
     8461 ggcgtctgcg tgacctgata ttcggctatg cggggatcgg tgcccagcac atgacgaaac
     8521 gcgatcgccg gcaccgtcgt tgcgccgtaa cagaagtcgt cgtcgcggcg cccgccgatg
     8581 tcagcgatgc gtgtgaaggc gctgccgcat gcgcactgac ccggcagtaa cgtgacctga
     8641 tcgccgaggt cgtagcgaat gaacgggaaa gtccgattgg ccagtccggt ggcaagcgtc
     8701 cgtgctgccg gctcgccggg cccgaccggc ttcccgttgt catcgacacg ctcgaggacg
     8761 acttcgtcct cgcagatgtg cagcccctgg tcgcgtccgc agccgaccgc ctgcaccccg
     8821 atttcggtgg acccccatag gttgtggatc ggtgcatgcc acgcctcgtg gatcgcccgc
     8881 tggtcctcgt ccagcagcgg ctcagagttg gtgctcacgc gtaccggcgc gattcggagt
     8941 tcgccggcca gggcggcgcg ggcaagccgc ccaatcacgg acggataacc caccaggtgg
     9001 gtgggtcggg caatcgccac cgaggcaagc acctcgtcga acggcgctcc cgcagcgatc
     9061 accaccgttt ccatgcccgg tgtggtcggc atatcgaaca acggcgtgct ggcatgcgga
     9121 ggcactccgg cctgcagcac cgcaagccgc gcgggaggcg agaccttcgt gccgcgaagt
     9181 tcttcgcgcg cctgcattcg ccatgccagg caggcagccg agacaaagaa ctgccaatcc
     9241 cacacgtaga caccgcgcac cccgctggag ccgcccgagc tgaagatctg ttgatctgct
     9301 gcggtatacg aaaaccattg ctgctcagca agaatgcgtt cggcgcggtc gcggtcgagg
     9361 tcgggtaccg tgacgatggc gtcccaccgc tcccgggcgt cctgctttgt catcatcgga
     9421 agcgacgcca aatcggcgac cgaggcacgg gccgggtcga gcccccgtag ccggcgggcg
     9481 tgaaacggtg accgctcccg cgcgaaggca agcagggatc gcagccgcca actctggtat
     9541 cgctcaatcc gctggcggga ccagtccagc cgcgtgacgt ggtcttcgag accggcctgc
     9601 accgcattca ggtgcgccgc ccgcatccgc tcgtaggctg acatcgcggc caatgcggac
     9661 ccctagcggt cggcgggagc catggaccgt gccggcccca acgccaccgg cagcgccgac
     9721 caaccgcgca gcacccgcgt gtcccgtcgg cttccggtgc ccgccgcgcg cgcctcgggg
     9781 aaccggtcga agaacgttcg gagcccgact tcgccctcgg cccgcgcaag ggcggcaccg
     9841 aggcagaagt gccgaccgcc ggagaacgcg agatgccttc ccgcatttgg tcgttcgatg
     9901 tcgaaccggt gcggatcggg gaacaccgac ggatcccggt tggcagcggc caggtaaacc
     9961 accaccagct cgccacgttg gatccgcatg cccgccaccg tgacgtcgtc gagcgccacc
    10021 cgcgcggtga gctgcaccgg cgaatccagc cgcaggatct cttcgacggc gttgagccac
    10081 aactcaggac gttggcgcag cgtgtccagg tgccagggtg cgtccagcaa catgcgaatc
    10141 ccgttgccca gcaggttcac cgtcgtctcg aagccggcgg ccaacaccag gcccgcgatg
    10201 ccctgcagtt cggcctggtc gagatgtgtt tcggccgagc cgctttcggc cgtctggatc
    10261 agctggctca tcaaattgtc gctggggttg cgccgcaact cccgcaggtg gccggcgagc
    10321 cagtagttga atccggcgat accccgctgc acgctccggt actgccgcca cggcaatccg
    10381 atgtccaggc tgggcgcggc cagctcaccg aattccagca cgcggcggcg gtcctgctcg
    10441 ggcacgccca ggatgtcgct gatgatggcg accgggagct gggagcaata ccgcctgacg
    10501 atgtccacca cgtcggaggc agaggagaca gaagagacac cggagacaga ggacgggttc
    10561 gccagctgat ccaacagatt gcccgccgtc tgttcgaccc gatcgcgcag cgcggcgacc
    10621 gcccgggggg tgaacacggc cgacaccgtc ttgcggtaac gggtgtggtc cggcggttcg
    10681 acggccagca acgagggcgc gcgcagcgga tgcaaaaggt cgtcgcgagt gcggcgctcc
    10741 agccaacgca acggtgccgg caggttcgac cccatgttca ccacgcggaa gtcatcggat
    10801 cgcagcagct catgggccag cgcgtggtcg gcggtcagat agttgacgcg gcccttcacc
    10861 aacgggccca gcgcccgaag ttcgtcgtag aacggcaccg gatccgccgc cagcgtcgga
    10921 tcggcgatca acctggcctg caagtcaccc cgccgcacac cgatcgccgc gacaccccgg
    10981 acaaacccgt gcatcgcgag ccagtgcaat cgctccttca ccggtcctcc atcggtcggt
    11041 ggtcttcacc cagcgtaggc cggtcggctg ggacgactca gcactcggcg gtgatcttga
    11101 agtccgtcgt caccgcttta ttgggatcgc tgctgttgat gccgtaggcg gtgccggtga
    11161 tggtgtacgc ggtgttggtg acgtcggcgt gcgcgtctcc caccccgcct tgccagaagc
    11221 tgccggtgaa cccgtcgatg ttgcggatct tcacccactg cgggatggcc cggtaaccac
    11281 tgagcaggac caccgcctcg acttggccgt tctggtcgcg caggtcgatg gtccggtagg
    11341 actgcagttg gctgcacgcg ggaggacgcg tcgtgcgaga gacgccgccg atgctgagtt
    11401 gggcgccttt gcgcggcgtc gtctccgcct gcgcgcaccc cgccgcgccg gcgacgacga
    11461 cgaaagcgac tgcgagcaac cggttctgca gtggtcgccc cttcactgcc gcttcggcat
    11521 cagtccgggc agggatctga cgatgcgacc ccgcacgaac tctgggctca gacccttgag
    11581 caggtcgatg aagcgaatgc tctcgggcac ataccaatgc aaccgcttcg gattctggta
    11641 ggcctgccac gccgcctccg ccacgctggt cgcgggcatc agccgaaaca tgcctttcgt
    11701 cggcgcgctg gcgcgcagtt cctcggcggt catcgtggcg cccgcgtcgt cggagtggtt
    11761 ggtggtgttg gtcaggatgg cggtgtcgat caggcccggg agcacgtcgg cgacccggac
    11821 accgtgtcgc tgccactcca cgctgagcgc ctcggtcaac cctttgaccg cgtgctttgt
    11881 cgccgagtag accgcgatgc gcggcatgcc gtaggtgccc gaggaggacg atgtcgagaa
    11941 catcaggctg tcgggcgtct tcttgagata gggcagcgcg gcgtaggcgc cggtcaacac
    12001 cgccttgaag ttaacctcga cgacgcgcat tgcggcgtcg tacggcacgt cctcgaacca
    12061 gccggattcg ccgatgccgg cgttgttcca catcatgtcg agcccgccgc cggcattgcc
    12121 gtcgcagaag tcggccaacg caccctccag ggcagccttc tccgtcacgt cgacggcgcg
    12181 gatccacagc cggccatcgg caagctcctg cttcagcgtc gccaggccat cgtcgttgcg
    12241 gtccaccgca cccacccgcc aacctttggc gtggaagagc ttcgctccct cacggcccat
    12301 gccactgccc gcgccggtga tgaagaccga tttcacgttg ccttctgcga cacgcggcac
    12361 gaaagccttt ccccggtcag cccgcttcga ccacattttt gatgcgcgtc agcgtcttgg
    12421 tcatatcgcg gatgttgcgg cgcttccgca gaaagccccc gaacagccag tacacgccca
    12481 gccacggcgc cgggttgagc cggaacgact cggtcacgtc ggtgccgccg ccgctgggcg
    12541 ccaatcggta gtgccagttg ttcaccggtc tgtcgccgag taacaccgcg aatccgaact
    12601 cacggccggg ctcgcaggcg gtgacctcgc acgtcgtcca gtacaccgac cctatctcgt
    12661 tgcgccggac gtgaccgcgg aatttggcac cgagctccgg gccggtcgca tcgcccagcc
    12721 actccgcctc gaagacttcg ggggagaacc gcgcggtgtt gcgaacgtca gcgatcagat
    12781 cccagatctt gcctgcgggc gccgccatgt gaacagtcgc cgagccttcc atggcctgat
    12841 ccaaacacag cttcgcgctc tgccatagcc cctttaacga ccacacaagc ccccatcaac
    12901 gaccacggaa gcccctatta gcggcacccg gcgctagtgc ggcatggccg cctgaatgag
    12961 gccgttgatg atctgcttga tgccctgtgc gggatgcgag tagaggccga tcggaagtcc
    13021 ggaggcggag ccgcacttcg gccacgggtc cagcccgtct tcggcgaaaa cccggttcgc
    13081 cactgcgatc tgctgatccc tggacgcggc cgcggggttg ccgacaccgc cgtactgggc
    13141 ccaggtgccc ggcttgaatt gcagcccacc gtattcgccg tttccggtgt tggcctgcca
    13201 gttgccaccg gactcgcatt gcgcgatggc gtcccagttg ggggccgcgg gcgcggcgtt
    13261 ggccacaccg gcggacgaag acaacgacac tgcaacgatc ccgcccacca tggcggactt
    13321 gacgagaggc ttgcagagtt ttctcatgcc aagcaattcg gccgccgtat ccgaagcgtt
    13381 accgatttga aaatccggcg agatacggtt ctagcagcgg aaatagacta aaaagccgca
    13441 aaacgagggt tttagaaatt ggtgagaatt aagtaaatca accctggcaa tatgactggg
    13501 tagccaacaa gagggcgcgc tgataaagac cgtcgagttg gcggtcgcgt atgacgtcgc
    13561 tcctggcgac gtcgagctgc gtcgggcagg ccggcgcatg caacagttcc cagtcggcgg
    13621 cgatctgcgt cagcatcgtt tggttgaggc cgtcgatggt tgaccgcgac gccgacaggt
    13681 ccgggggcga acccggcacg gccgccggat tcaatttcca ctccgcgaac cggctgtgtt
    13741 cgatcgcctc ggtggcgttg atctgatcac cgaagacacg cgtgacgtaa cccggatcgg
    13801 ggctgttggc ggagctcaac gcgcgcagct cctcctggac gcgagccgga tcctcgatgg
    13861 gagcctgcga acgccatttg aaggcggcca ccggctcggc gacctcgagc cgctgggcgg
    13921 cggcgtcgac caggtcggtg agcgccccgg cgggatcggc ccgtgcctgc gctgccggca
    13981 gcaccgcgag cagcaccgcc agcccggacc acgcgactcg ggcggcgtgg cggtgggtgt
    14041 cggtcatgcc gatgattctg acccgcttcc ggcccggatc gctccacccg aaaaagttcc
    14101 gccgatcgtt ttcttcggag ttagcctggc cgtagatgcc ctggactgtt gacatgcgcc
    14161 gccccggcga ggtcggcggc aaacgcgcag aatggttgcc tgcgtggcag gttcgcgcgc
    14221 tgacggcggc ggcgctgacg atctcgttgc tgctgggcgg ggcgttggtt ctggtggaca
    14281 ggctgcattc gaccacgtcc gacgtcctcg accatcccgc cgacccggtc accgacgacc
    14341 agtccgaggc tcaggtggtc gagtcgacca aacaaatcgt caccctgacc gggctgcgga
    14401 ccacctcggc gggctacacg ttgatgtcgt gcaaggaccg ccaagacccg ccttatcagg
    14461 gcgcgatcta cctgaacttc gcgctgccct cggcaacccg ccccgacacc tactttccga
    14521 cgatcgcggc gacgctcgtc agccacggct ggaccgaggg cctgccaccc aacgatcacc
    14581 cgttcgccag gatcttctcc aaggacgccg tcactgtgac tgtttatcgg cacagcgatg
    14641 acccgggcct gggtgtcctg cgtgcctacg ggcagtgccg gaatatcaac gatcaccata
    14701 aagacgcgac gacgtggacc gatgtcaccg cccagttcac cagtagcgcg tgaggatccc
    14761 taacgcgcta ctgcgcccga cgggctgtcg tcaggcgcag cagcgatcgt ccgggagggt
    14821 gcggggttat ccgccgccac cgggcgtggc gcccaaggtg ccctgcaggt cgggcttcat
    14881 ggcgttgagc tgggctcccc agtactccca gctgtgcgtt ccgttggcgt tgaagttgaa
    14941 cacggcgttg tgaccgccgg ccgcgttgta ggcgtcctgg aacttcaggt tgctgctgcg
    15001 aacgaagttc tccaggaact cggccggcat gttggccccg cccaactccg atggcgttcc
    15061 gttgccgcag tagatccaca gccgggtgtt gttgccgacc agcttgggga tctgcaggct
    15121 ggggtcgttg cgctgccacg cggggtcgct cgacggtccc cacatgtcgt tggccttgta
    15181 gccgccggcg tcacccattg cgaggccgat cagagagggc cccatcccct gagaggggtc
    15241 catcaaagcc gacagtgagc cggcgtagat gaattggccc gggtggtaag cggccagaat
    15301 catcgccgag gagccggcca tcgagatgcc gatcgcggcg ctaccggtgg acttgacgct
    15361 cctattcgcg gacagccagc ccggcagctc gctggtcagg aaggtctccc acttgtaggt
    15421 cgtgcagccg gccttgccac aggccgggtt gtaccagtcg ctgtagaagc tggactgccc
    15481 accgaccggc atgatgaccg acaggcccga cttgtagtac cactcgaacg cgggggtgtt
    15541 gatgtcccac ccgttgtagt cgtcctgtgc ccgtaacccg tcgagcaggt acaccgcggg
    15601 tgagccgtct ccgccgcttt ggaactggat cttgatgtcg cggcccatgg ctcctgaggg
    15661 cacctgcagg tattcgactg gcaggccagg gcgcgagaac gcccccgcgg tcgccgcgcc
    15721 cccggcaagg ccgatcaggc ccggcagggt gacagccgcc gccgcaccga ccaaaagccg
    15781 ccgcccccag gcgcggatct tctcgctcgc ttctgtcata cctgcgcccc tgtcgtttgg
    15841 cttgtcgtgc cctcggctgg aatttaccgt gctaatgcgc caaatgtcga ttgggacacg
    15901 aagtagtctc agccgagtta cggattccgc cccgaatttc acaactcgga taagcggacc
    15961 gcaaaggtcg ttcaggtccc cgcaccagcc tcggccgccg cggccccttt cacaaaaacc
    16021 ccaggcagca ggcccgcgcg gggcgggcgc gaccggaaca tcggcggctg gctgcgtaag
    16081 ggacttcgaa accaaacggc ttcggatcga aatgggcgag gcgatttcaa cgagcggcgt
    16141 cacggggcgt cggcgtgcca cccccagtgg cgtcgaagtc cgcctcccac gcgttcgatt
    16201 gccgccgtcg aaccgtcgac gcccacgacg gaattatttg tcggggcggt aaatagttga
    16261 gcggggcggc ggaagcggaa cggcggagcg cgtaaagcct tggccgactc cggcgaaata
    16321 cggcgccgcc gagtgccgct tgtctgccgg cctgcgcgta tgaccgggat ttcggaccgg
    16381 ggaagccgaa gttgcgcgga ccgcgtaggc tctgtcgcag caagccgatg gagaccacgc
    16441 aacccggtca attccggcgc tcacgaacca ccgcaccggc ggattcatgg tgcgcgttgt
    16501 gcggctgagg gcgtcggctg tccgggaccg attgaggtgc agattttgga cgcggtactt
    16561 gggctatccg tgacgccgac caccgtcggg tgggtcctcg ccgaaggaca cagcgcagac
    16621 ggcaccatcc tggaccatcg ggaattgccg ctgcatgccg gcgatggagt ggccgcagtg
    16681 agtaccgcag agcaagtggc cgacgaggta ttgcgggtga acgagcttgc ggcggcgagc
    16741 gatcaccatc tgcgtgtcat cggactgacc tggaacgatg aggcgtccgc ccaggcggcg
    16801 ctgttggtgg aggcgctgac cgacgcgggg ttcgacaacg ttgtgccggt tcggctgatc
    16861 gaggcggtgg agaccctggc cagggcgatc gcaccggtca tcggttacga gcagacggcg
    16921 gtctgcgtcg tcgagcccga gtgggcgacc gtcgtcatgg tcgacaccca caatggcgag
    16981 acccagacgg tcgtcaagca cgtccggggc ggcttcgacg gcttgacccg ctggctcagc
    17041 ggaatgttcg accaggacgg atggcgccct ggtggtgtgg ttgccgtcgg cgcggacagc
    17101 gaaatcaacg gattctcgtg gcagctggaa aaggctttgc ccataccggt tttcgcgcag
    17161 accatggccc aagtcacgat tgcgcggggc gcggcactgg ccgcggccgc aagcaccgac
    17221 ttcaccgacg agctactggt ggcgcacatc ggtgagcccg ccgtggcgcc tgcgcggccc
    17281 cggaagttgt cgcacgccgg agcgctgacc gcattggccg ccggtgcgat cacgttcgtc
    17341 acgtcgctat cgctcgcagt gggtattcag cttgccccga caagcgagcc cgcacagaag
    17401 cacgtagtgc acgagccgac gccgcagatc gcggaggccg ttgcaccgcc cgccgaggcg
    17461 gcaccgccgg cgccccagtc aaaggcgccc gcgggcgcac cggctcagga ggcaggcgcg
    17521 ccgggcgacc aggatggcgc tcccgccgac cagcaatcgg acccgaacgc gcgtcagccc
    17581 tacctgacgc gggtgctgga gcacatcccc ggcgatgccg gtgattctgc ggcaccgacc
    17641 cagccgcagt cgccgtagtc gccccggtcc caacgagggt ggcacgacgg ttccggtcgc
    17701 gccggaccgc ggtgcagccg cgccgagcgg aactgccagt ggagttcagc cgtccttggc
    17761 gtgaaccgcg gtgaacgaca aagacacctc gtcggcaacc tgcagggagc ccatcagcag
    17821 cgagtagggc ttgatgccgt aatccgtttg ccggaccgcg gattcgacag acattcgcca
    17881 cgccgtaccg agatcctctg tgcgcaggtc gattacctgc tctcgggacc tgccgcgaat
    17941 gtgcagcatc ccggtgagcc ggtacccgtc ggcgatcttg gctatctgct ccgcgccgtc
    18001 cgcgataaag cggatctggg gatagtgcgc ggcgtcgagc gacttcatcg cgttggactt
    18061 caccagtacc ttttccggcc cggacaacgc cttgactccg ccctcgccgc gcagcacctc
    18121 aaaggaatcg atctcgacga tcagctccgc tgtgaccggc tcggcgcccg cccaccccac
    18181 cgtggcctgc cagcgcttca tcgcgatcgt gagccgatga cccattcgcg ccgccctgcc
    18241 ggcgacgccg gtgtgaatca ccaattcacc gtcggatgcg tccagggtcc acgcgtcggt
    18301 cacagaccga ctgtagtcac atggtcaaaa cggtgcggta gtgcgcgcgg ccgtgttcca
    18361 tggccgcgta gccctccgcc gcctgggcca gcggcagttc ctgggtccac gcccgcacgc
    18421 ccgacaagac cgcgaaatgc attgtgtctt cgacgtcttt cgacgtgccc gagggatggc
    18481 cgacaatgct gagtcccggt gtgatcagct gcaccggact gatcggcagc ggttcgggag
    18541 tgacgccgat ggtgaccagt tcgccttgtg gcagcagccc gccgaccgtc gcggccatgg
    18601 cgggggagtt gccggcggtg gccagcacaa ccgcggcgcc gcccagcttg cgcatggctt
    18661 cggccacgtc accgctggtg gagtcgatgt agtggtgagc gcccaattcg cgcgcgtctt
    18721 cggctttggc ggtgccgcgg gcgatcgcga tcgtttcgaa acccatggcc cgggagaact
    18781 gcacgccaag gtggccgagc ccgccgacgc cgaggatcgc gacccggtca ccgggcaacg
    18841 ccttggtgtg ccgcagtgcg ttgtaggtcg tcacacccgc acagcccaat ggggccgctt
    18901 cgacgtcgga gagctcggag ggaatccggg ctaaggcgtt ggccggcgcg gtcaccgatt
    18961 ccgcataccc gcccgggtac tgccaactgg gcaccttgcc gttgacgcag tggatgaacg
    19021 agcctttgcg gcaggggtcg caatgaaagc agcaaccgcc gaaccatccc acggcgacac
    19081 gatcaccgac cgcgaactcc tcgacgccct caccgagttc ggcgatggct ccggcgattt
    19141 cgtgtccggg ggtcagcggc cacgacatgc cgggaaagcc accctcgaca aaatgctggt
    19201 cggtgccgca gatgccgcag gcggtgacgg tgaggcgcac ctcgccgcgg ccgggcggca
    19261 ccgtctccac atccaccagc tccagggcgg cgcccgccga cgagacctgg acagctttgt
    19321 gcgtggccat acgcctacct cgccgttcgg tttgctagtg ggcgacaggg catttggctg
    19381 cccggtcggt atggtaatcg acctgccagt gcttgatccc gttgagccaa cccgaccgca
    19441 gtcgctcggg cttgccgacc ggttccagat cgggcatggc gtcggcgatc gcgttgaaca
    19501 tcaagtcgat cgtcatccgc gccaggttgg cccccacaca gtagtgcgca ccggttccgc
    19561 cgaatcccac gtgcgggttg gggtcgcgca ggatgttgaa gctgaacggg tcgtcgaaga
    19621 cgtcctcgtc gaaattggcc gagcggtaga ccatcaccac ccgctgcccc ttcttgatcc
    19681 gcacaccgga cagttcgtag tcctccagcg cggtccgctg gaacgacgtg accggggtgg
    19741 cccagcggac gatctcatcg gccgtggtga ccggacgcct tcttttgtag agctcccact
    19801 gatcggggaa gtcggtgaag gccatcatgc cctgggtgat ggaattgcgg gtggtctcgt
    19861 tgcccgcaac cgccaacaga atgacgaaga agccgaactc gtcgtcggac agtttgtgtc
    19921 cgtcgatgtc ggcctcgacc agtttggtga caatgtcagg cccggggttg ttcgcccggt
    19981 cggcggccat ttgcatcgca taggtgatca gttggatcga cgccgtgaga ccgtcattgg
    20041 tggcgaactc gggatcctgg tcgcccacca tctggttgga ccactgaaag agcttcatgc
    20101 gctcctcctg cggcacaccc agcaatccgg cgatggcctg caacggaagc tcgcacgaga
    20161 cctgctcgac gaagtcaccg gaaccttcgg ccgccgcggc ctcgacgatg cgcctggcgc
    20221 gctcgttgag gtcgtcgcgc aaccgctcga cggcacgggg ggtgaagccc cgggagatga
    20281 tcttgcgcag ccgggtgtgc tgcggcgcgt ccatgttgag cagcacgaac ttgccggtgt
    20341 cgatctgctc gcggaccgtg ccctccttgt agcgggggag ggcggtcttc tgcaggctgg
    20401 agaagacatc gctgcgcagc gagacctcct tgacgtcctt gtgcttggac accacccaga
    20461 agcctccgtc gtcgaagcca cccgtgccga taggctgttc gttccaccag atcggagcat
    20521 ttcggcgcat ctcggagagt tcttccaccg gcagtcgctg ggcgtagatg tcggggtcgg
    20581 tgaagtcgaa tccgggcggc agattgggaa tcgccggaga tggcatggtg acccactcct
    20641 tcaatacgaa gtcttctagt cgtcctgctc tgccaaataa accattgcca caccacggtt
    20701 gggaagcacc aacgcgagat cgccaaagcc gaactgcaac gtgttgcagc cgcgatgagt
    20761 ttccggcatt gcgccggtct gcaacaccga cacaaccgag caactgaaag gacatggccg
    20821 atgcacatcg ttttgagcga cttcatgagt ctggacggag tcgtccaatc cccgggcgcc
    20881 caaggtgagg acaccgacgg tggattcgca cacggcggct ggtcgatgag gtacttcgag
    20941 cccgaaacca tgggtccggc ggtcaccgag accatggctt ccaccgatgc cctgctgttc
    21001 ggccggcgca cctggcaaac gatggcggcc gcctggccga cccgcgccgg ggacccgttc
    21061 gccgaccgga tgaacgagat gcccaagtac gtggcgtccc gcacgctcac ccgagacgac
    21121 ctcacctggc ccggctcgac tctgctgccg gccaacgacg caacgggcgc gatcggcgcg
    21181 atacgcgagc tgcgcgaaca agacggaggc gcgatacagg tgatgggtag cgcgtcgttg
    21241 gccgcgcagt tgatcgagca tgacctcgtc gacgaatacc gcctgatgat cgagccggtc
    21301 ctgctcggcg gcggcaagcg agtgttcccg gacgacggcc gggcccgcgc actcgagctg
    21361 gtgtcggcga cgacaaccgg gaccggtgtt ctgatctgca cataccggcg tgtttgacca
    21421 gcgtgggtgc ccgcaaactc ctaccgcgaa aacgaccttg tggcacgcgg cacagtcgat
    21481 atgctttggt ccaggtcacc ggcgcgccga ggggccggat gacgctgacg cgaagggtgt
    21541 aacgcatgat ccgcgaattt ctcgccgtcg ctgcgctcgc aggcggcacc gtcagtgttg
    21601 cgccgtgcgc ggccgccgat ccgcattacg acggtgacgt gccaggcatg aactatcagg
    21661 cgttgctcgg tgcgccgtgc gacaactacg agcgcttcat cttcggacgg ggccccagcg
    21721 gccaggccga ggcctgtcat ttcccgccgc ccaaccagtt cccggcggcc accatcggct
    21781 actgggtcat ctcctacccg ctctacgggg tccaacaggc cggcgcgaag tgcccgggct
    21841 cgcaagctgc ggctcagtcg gccgccggac ttccgatgct gtgcctggga aaccaagggt
    21901 ggcaagaggg atggttcacc ggggcagggt tcttcccgcc tgcgggatga ccggctgtcc
    21961 tccggcccag cagtacggcg cgcccgcttc tccacgagcc acgtcttgga tgcggtatca
    22021 aggaggaaga ggtgaatagt cgtgcacaat tacccctcct acccctcggt cgaccgtccc
    22081 gccggatccc cgatcccacg ctggctgcgc ttcgtcctcg cgtccgaccg agccggctcg
    22141 gcctggtaca tcgggaccgg ttttttcttc gcgccggcgc tcgccctgct gtcgccgtgg
    22201 ccggcggtca ccgccgtgct ctggggagtg atcgggctgg ccggactgtg gttgggcctg
    22261 ctcggcgtcg cgatggcggt ggggctggcg cggatattgc ggtccggcgc cgagatcccc
    22321 gaagagtatt ggcgcacctt ggtgaactac tgactaacag ggaccgcggg agccccccgc
    22381 tacgtctaga gtcgaaccga aacttgtgca acgaacggag atttccatga ccttcaatcc
    22441 caaagacgca gtcgacgcgg ccaaggacat tgcgaccaat gcggtcgaaa aggcttcgga
    22501 tatcgtcgaa cacgccggcg acatcatccg cggcgacatc aagggcggcg ccagcgggat
    22561 cgtgcagaac tcgatcgaca tcgcgaccca cgcggtcgac cgggccaagg aagtgttcac
    22621 cggcaaaggc gaagccgacc ccgagtaagc gggctgaaaa ggccccagcc ggccccagcc
    22681 ggcctagacg gcggccgcag cgttcagctc atccagcctg gttgtcgcgg cgaggtactc
    22741 ctgcacccag cggtcgatga cggccgccgt cttttccacc ttggtgaact ggccgacaac
    22801 ctgaccgacg gggttgaacg ccacgtcgac ggtctcgttc gggtacttat tggtggcacg
    22861 cacggccatg ccggacacca tgtactgcag cggcatgccg agcggcttgg ggttgtcggg
    22921 ctgttcccac gcctcggtcc aatcgttgcg cagcatccgg gccggtttgc cggtgaacga
    22981 acggctacgc acggtatcgc ggctgcttgc cttggcgtag gccgcttgct gaaccggggt
    23041 gttcgaggct tcctcgacca tcagccactg cgagccggtc cacgcccctt gggcacccag
    23101 cgccaaagcc gcggcgatct gttcaccgct gccgatgccg ccggcggcaa ggaccgggac
    23161 tggtgcgacc tccttgacga cttgcggcca caacacaatt gaccccacct cgccgcagtg
    23221 tcccccggcc tcgccgccct gcgcgatgat gatgtcgacg cccgcgtcgg cgtgcttgcg
    23281 ggcctgcgac ggtgagccgc acaacgcggc caccttgcgg ccggagtcgt ggatgtgctt
    23341 gatcatgtcg gcggggggag tgccgagtgc gttggcgatc atcgccacct tcgggtgctt
    23401 gagcgccatt tcgacctgag gggtggccgt cgcttccgtc cagccaagca attgcaaggt
    23461 gtcctcatcg gcgttttcca cggggacacc gtgatcggcg aggatctttt tggcgaagtc
    23521 cagatgctgc tggggcacca ttttgcgcag cgtttcggcg agctcgtccg cggacaaatg
    23581 cgagtccatg ccctcgtact tgttcgggat cacgatgtcg acgccgtagg ggtggtcgcc
    23641 gatgtgctcg tcgatccagt tcagctcgat ctctagctgc tcgggcgtga acccgaccgc
    23701 gcccagcacg ccgaaaccgc cggccctgct caccgcgacc acgacgtcgc ggcaatgggt
    23761 gaaggcgaag atcgggaact cgatagcgag gtcgtcgcaa atcgcggtgt gcatgcgtgc
    23821 tcctggaaag cttgacccgg tgaaaagaga tccgaaacga aatggaacgt gttctagttt
    23881 agtacggacg tgattgacgt ggccagccgc ccttgatcag tgcgtcgcaa ctacgacgcg
    23941 tcacccgtca gaccgtcgcg gtgtaggcga tccacaggac gaggaacgtg agcatgcagc
    24001 ccgcgcagat caggtgatcg cggcagatgg atcgcgccag tcgggtctgt tcggcggcgc
    24061 tgtccgcgcg gtgtccgagt tggatcgcat tggggaccgt gtgagtcaag gccagcgcca
    24121 ccgggacgcc cgcgagcgcg gccgatatcg gcagcaacca cgccggatcg ttcccccgag
    24181 cggcctgaaa tgcgagcgcg cccaacagca cgaccatgac aacgacgatc agccggctca
    24241 ttggtcgcga tgtggtcgtt gcgcgatggt agtaacccgc gatcgacgcg aggacgggct
    24301 cgggcaattc ctcgccggca tcgcggtgtc cgagaacctg gacgtcgaaa atgagatcca
    24361 tccacaagac ggcgagcagg aatccaccgc atgccgcgag cagcgtggcc accacgctca
    24421 cctcccgttc gccgactgac cgccgcccgg cgcggtgcgc caccattgcc gcgggcggac
    24481 gacgaccgcc ttattgtttc gcagcgctcc gctgcgtccc agagcgggtt ttgccggcgg
    24541 gccggtccgg tcgcgacgat ttcgctcaat caccgatgtt tgggtggtcg tgaatttgcc
    24601 gcgcggggtt gcggggatgg cacgatatcg aggtgagcga cgcgcggaaa ggcgacgcgg
    24661 gaaacgggct gcaccgccag cgcggcgccc cgaccgacgc gttgctggat cgcgcggtgg
    24721 ttccggtggg ggtggtgacg ttcctgttca ccgatgtgga gggttcgacc cgtcggtggg
    24781 aggccgacgc cgacacgatg cgggtcgccc tcgccgccca cgacgaagta ctgcgccaag
    24841 caatcgaggc gcacgggggc tggatgttca agcacaccgg cgatggggtg tgcgcggcgt
    24901 tcgcgtcgcc gcggtccgcg ctggatgccg cggtggccgc gcagcgggcg ctcgagttgc
    24961 cggtgcggat gggcatcgcg accggcgagg cggagctgcg cggggcggac tacttcggcg
    25021 tggtgctgaa ccgtgccgcg cgggtgatgg ccgcggggca cggcggtcag atcctggtgg
    25081 ccgattcgac cgcgggactg ctcagcgggg ccgacctcgt cgacctgggc gcgcggcggc
    25141 tacgggatct gccgacggcg atcgggctct tccaggtccg cgccccgggc ctgcgcaccg
    25201 agttcccgcc cccgcgcgtg ctcgattcga gcgttgggaa tctgcggccg caggcgagca
    25261 gcttcatcgg ccgcgaatcc gagcttgccg aggtgcaagc gtcgctgcgg ggccgccggt
    25321 tcgagacgct gacgggggtg ggcggggtgg gcaaaacccg cctggctttg gaactcgcca
    25381 cccgcctggc cgacgaattc ccggacggcg tctgggtttt cgaactcgcc aacgtcagcg
    25441 atccggccgc ggtgcccgac gcggtggcgt cggtgctggg cgtcagccaa cagccgggga
    25501 agaccttggc cgacagcgtg gccatcgcgc tggaaggcag ggttcggctg ctggtgttcg
    25561 acaattgcga acacgtgctc gacgccgcgg ccgatctgat cgagaccatc ctcaggcact
    25621 ccacgacggt gacgatcctg gccaccagcc gggagaggct ggggctcgcc caggagcagt
    25681 tgtggccggt gccgtcgctg gatgtgcgcg ccgggatcga ctccgccgcg gtgactttgt
    25741 tcgtcgaacg cgcccgtagc gtggcgccga atttctccct gaccaggccc gacgaggcga
    25801 gcgcggtgct ggagatctgc cgtcgcctgg acgggattcc gttggccatc gagctggcgg
    25861 cctcgcgaat ggcgtcgatg acggcgatcg aggtgcgcga tcggatggat cagcggttcc
    25921 ggctgctggt cggatcgcgg cgtggcctgg gccgccacca gacgcttcgg gatgcggtcg
    25981 cgtggtccta cgattacctc gacgatgccg agaaggcgct gctggaacgg tgttcggtgt
    26041 tcgccggcgg gttcgacctc gacggcgcat gcgcggtcgt cggccccgac ggcccggacg
    26101 atctggccgt cctggatctg ctcgatgccc tggtgcgcaa gtcgctgctg gttgccgatc
    26161 ggtcatcggc gcggacccgg ttttccatgc tggagacgat ccgccagttc gccgacgcgc
    26221 agctcgtcgc aaccggcgcc gcgacgcagg cccgggccgc acacgcgcgc tacttcgccg
    26281 ggcgcgaagc cgacgtcctg gccctgtggg atagcccccg gcaacgggag gcctacacct
    26341 ggttcatcgt cgagctggcc aatctgcgca cggcctttag gtgggccgcc gagcagggcg
    26401 acctcgacgt cgccaccacc atcgcgacgt acgcgacgtt cctcggcctg tgggtggaga
    26461 actacgagcc gatcgcctgg gccgaagagt ccatcgagcc cgccctcgcc gtcgaccatc
    26521 cccggttggc gttcctgtac accttggcat cgcactgctg gtatccggga cgcctggagg
    26581 aggccatcca ctacagcgac gccggacagg tggtgatgcg cagcgacggc cgcggggtgc
    26641 cgttcggctt cgagggtgtg ctgcgcaatc catacctggc catcggccag cccggacgat
    26701 gcgccgagtg gtgccgggcc cggctcgccg acggtcccga cacccacaca ttcaccaggg
    26761 cgaccctgac catcgcgctg acgatggccg gtgccggtga ggaggcccgg gccgccgcga
    26821 acggcctgat cgacgcggcc gacgccaccc acaatccata cacgatctcg ttcgcgctgt
    26881 gggcctacgg ctacaccttc cgcgacgccg ctcccgccga agcgctggac gcgatgcgcc
    26941 ggggcctggc gatcgctcag gacagcggca accggttgac cgagacacgc ctgaccggga
    27001 cgctgtcccg gctcgaggcc cagtacggtg atccgctggc cgcgctcgac tacctcacgc
    27061 tggtgatgcg caattaccac gacgcgggca acaccgccat gatgcgcacc ccactggcgg
    27121 tcctcgccgc gttgatcgac cggctcggac gctacgaacc ggcggccgcc atcgccggtt
    27181 tcgccgccag cccactaacc gcggtggccg tccccgagtt caataccgcg atcgcccatc
    27241 tgcgcgatgc gctgggtggt ccggcctacg aatcgatcgc ccggaagggc caacagatga
    27301 ccaccgccgc catggccgcc tacgcctacg accagatcga tcaggcccgt acgggactca
    27361 cacacccgaa ctgaaccgcc ggaccgcgac gttcccgcaa ccgggacccg gaaacgggtc
    27421 ttgatctgct ccagcgcagg ggggtagcgt tgtgctgcca attacgaaac tatgcgtagc
    27481 gtaatttggg acgggtctca atttcttacg cgacagaacg agtgagtgcg acacagaaag
    27541 tgaacacgcc actatgcgca ccgtagttgt cgatgggacc cgcagcatcc gggtcgatat
    27601 ccgtcccgat cccgcgcttc cgggggccga cggcgcgatc gtctccgtca ccgccgcggg
    27661 catctgcgga tccgacctgc acttctacga gggcgaattt ccattcgccg agcccgtcgc
    27721 cctcggccac gaagcggtcg gcaccgtcat cgaaaccggt tcacgggtaa gcactgtcga
    27781 ggtaggcgac cgggtcatgg tgtcctcggt ggccgggtgt ggctcctgcc cgggctgcgc
    27841 caccaaagac ccggtcatgt gttactccgg cctgcagatc ttcggctccg gcgcgctcgg
    27901 cggcgcgcag gccgatctgc tcgccgtgcc cgccgccgac ttccagctgc tcaagatccc
    27961 ggagggaatc aacgacgagc aggcactgct ccttaccgac aacctggcca ccggttgggc
    28021 ggcagcgcag cgggccgaca cctcgttcgg cagcaccgtg acggtgattg gcctgggagc
    28081 ggtgggcctg tgcgcgctgc gcagcgcatt tgtccaaggc gccgcaacgg ttttcgctgt
    28141 cgatcgggtc gacggccggc tggaccgagc tgcccggtgg ggcgcaacgc cgctcaaggc
    28201 gcccgcggcc gaggccatca tcgccgcgac ccgaggccgc ggtgcggact cggtgatcga
    28261 cgccgtcggc acggacgcat ccatgaccga cgcgctcaac gcggtgcggc cgggcggcac
    28321 cgtctcagtt gtcggtgtgc acaatctgca accgttcccg cttccggccc tgtcctgctt
    28381 gatgcgcagc atcacgctgc ggctgaccac cgcgccggtt cagcgcacct ggccggagtt
    28441 gatcccactg ctgcagtccg gccggctcga cgtcgacggc atcttcacca cgacactgcc
    28501 tttggacgaa gccgccaaag gttacgcgac cgccgaatcg cggtccggcg acgtcgtgaa
    28561 aatcctgctg cggccttagt cagcttccgt cccggcggac gtgcttggcc agggaacgca
    28621 aggcccgcac cggcccgcgg cggccggacg caaggcaca
//
LOCUS       DASSGQ010000387         3267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1214833,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000387 DASSGQ010000000
VERSION     DASSGQ010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214833"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..947)
                     /locus_tag="VFI55_17115"
     CDS             complement(207..947)
                     /locus_tag="VFI55_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HET7075783.1"
                     /translation="MTALVLTAHGSRDPRSAANARAVARRVAAMRPGLEVRLAFCELN
                     TPSLVDVLDGLPDCGREAPKAIVTPLLLANAYHARIDIPRQIAGCPAAERVWRAPVLG
                     EDPRLVSLLRRRVVELGVSRLDERLGVLVVAIGSSDPVANARTAQVAAKLMAGTRWAG
                     ATTAFVTRPEPSLAEGVDELHRRGARRVVIAPWFLAPGRLPDRVRVFANDAGIDMAQP
                     LGAHGSVAETVLDRFDRALKARAEHVAA"
     gene            complement(944..1684)
                     /locus_tag="VFI55_17120"
     CDS             complement(944..1684)
                     /locus_tag="VFI55_17120"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HET7075784.1"
                     /translation="MTNATRRTEPELRELAARGAAELEGASATDLLRWTEDNFGGVNG
                     PRGWATCNYVVASNMQDAVLVDLAARVRPGVPVIFLDTGYHFVETIGTRDAVESVYDI
                     RVLNVTPEQTVSEQDELLGKDLFARDPGECCRMRKVVPLGKTLRGYSAWVTGLRRVEA
                     STRANAQLISFDEAFKLVKINPLATWTDQDVQDYIAEHDVLVNPLVHEGYPSIGCAPC
                     TAKPVEGADPRSGRWQGLAKTECGLHAS"
     gene            complement(1681..>3267)
                     /locus_tag="VFI55_17125"
     CDS             complement(1681..>3267)
                     /locus_tag="VFI55_17125"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003899303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HET7075785.1"
                     /translation="EMKQAGAPLDVRERIENVYAKDGFDSIDKSDLRGRFRWWGLYTQ
                     REQGYDGTFTGDENIELLEAKYFMMRVRCDGGALSAAALRTLGQISTEFARDTADISD
                     RENVQYHWIEVENVPEIWRRLDEVGLQTAEACGDCPRVILGSPLAGESLDEVLDPTWA
                     IDEIVRRYIGKPDFADLPRKYKTAISGLQDVVHEVNDIAFIGVNHPEHGPGLDLWVGG
                     GLSTNPMLGQRVGAWVPLDEVPEVWAAVTSIFRDYGYRRLRAKARLKFLIKDWGVERF
                     REVLETEYLKRPLIDGPAPEPVKHPIDHVGVQRLKNGLNAVGVAPIAGRVSGTVLTAV
                     ADLAERAGSDRIRFTPYQKLVILDVADDKLDGFVSGLEALGLQTRPSHWRRNLMACSG
                     IEFCKLSFAETRVRAQGLVPELEGRLEDINSALDVPVTVNINGCPNSCARIQIADIGF
                     KGQMVDDGHGGSVEGFQVHLGGSLGLDSGFGRKLRQHKVTSDELGDYIERVVRNFVKH
                     RAEGERFAQWVVRAEEGDLR"
BASE COUNT          539 a         1144 c         1036 g          548 t
ORIGIN      
        1 atccaggccc tgaccacaca gctccacaaa atgaccacca gcaagtttca acccgccacc
       61 cgggcccgag tccccaagcg cgcacaatca catgaggcaa cggaaccgcc ctcgcgcgca
      121 tcttgacgtg aggcaacagg caccgacaaa accttcgccg tgaaatcgct gaaactaaag
      181 tggcacaagt ggtttgatct gaccgatcag gccgcgacgt gttcggctcg agccttcagg
      241 gcgcgatcaa agcggtccag caccgtctcg gccaccgacc cgtgcgcgcc cagcggttgc
      301 gccatgtcga tgccggcgtc gttcgcgaaa acccgcaccc ggtccggcag ccggcccggt
      361 gccaggaacc acggcgcgat gaccacccgt cgggcgcccc gtcggtgtag ctcgtccacg
      421 ccttcggcca gcgaaggttc cggacgggtc acgaacgcgg tcgtcgcgcc cgcccaccgg
      481 gtgcccgcca tcagcttcgc cgctacttgt gcggtgcggg cattggccac cgggtcggat
      541 gaaccgatcg ccaccacgag cacacccagc cgctcgtcga gacgggaaac ccccagctcc
      601 actacccgtc ggcgcagcag tgacaccagc cgcggatcct cgcccagcac gggcgctcgc
      661 cacactcgtt ccgcggccgg gcagccggcg atctggcgcg ggatgtcgat gcgggcgtgg
      721 tacgcgttcg ctagcagcaa cggggtgacg attgccttgg gcgcctcgcg gccgcagtct
      781 ggcaggccat ccaggacgtc gaccaggctg ggcgtgttga gctcgcagaa ggccagccgc
      841 acctcgaggc ctggccgcat ggccgcgacc cggcgggcga cggcccgtgc gttggccgcc
      901 gaccgtggat cccgactccc gtgggccgtc agcaccaggg ccgtcacgag gcgtgcaaac
      961 cgcattccgt cttggccagt ccctgccagc gtccgctgcg cgggtcggcg ccctcgacgg
     1021 gcttggccgt gcagggtgcg cagccgatcg acggatagcc ttcgtgcaca aggggattga
     1081 ccagcacgtc gtgctcagcg atgtagtcct gcacgtcctg gtcggtccag gtggccaggg
     1141 ggttgatctt gaccagtttg aacgcctcgt cgaagctgat cagttgggca ttggcgcgcg
     1201 tcgacgcctc gacccggcgc agcccggtga cccaggcgga atagccgcgc aacgtcttac
     1261 ccagcggcac caccttgcgc attcggcagc actcgcccgg gtcgcgggcg aacaggtcct
     1321 tgcccagcag ttcgtcctgc tcggaaaccg tctgctccgg agtgacattg agcacccgga
     1381 tgtcgtagac cgactcgacg gcatcccggg tcccgatggt ttccacgaag tggtagccgg
     1441 tgtccagaaa gatcaccggt acgcccggtc gcaccctggc ggcgagatcc accagcacgg
     1501 cgtcctgcat gttggaggcc accacgtagt tgcacgtcgc ccagccgcgc ggcccgttca
     1561 caccaccgaa gttgtcctcg gtccaccgca gcaggtcggt ggcgctggcg ccctcgagct
     1621 ccgcggcacc ccgagcggcc agctcacgca actcgggctc ggtacgtctg gtcgcgttgg
     1681 tcatcgcagg tcgccttcct cggcgcgaac gacccactgc gcgaatcgtt caccctcggc
     1741 gcggtgtttg acgaagttgc gcaccacccg ctcgatgtag tcgcccagct catcgctggt
     1801 gaccttgtgc tgacgcagct ttcggccgaa accgctgtcc aggccgaggc tgccgcccag
     1861 atgaacctgg aatccctcga ccgagccacc gtgaccgtcg tcgaccatct ggcccttgaa
     1921 cccgatgtcc gcaatttgga ttcgcgcgca cgagttcggg cagccgttga tgttgacggt
     1981 taccggaaca tcaagcgcgg agttgatgtc ctcaagccgg ccctccaact ctggcaccaa
     2041 accctgtgcc ctgacccggg tttcggcaaa ggacaatttg cagaactcga tcccgctgca
     2101 cgccatcagg tttcggcgcc agtgcgacgg ccgcgtctga aggcccagtg cctcgaggcc
     2161 cgagacaaac ccgtcgagct tgtcgtcggc cacgtcgagg atgaccagct tctggtatgg
     2221 ggtgaaccgg atccggtccg aaccggcccg ctcggccagg tccgccaccg cggtgagtac
     2281 ggtgccggag acccgtccgg caatcggggc gacgccgacc gcgttgagcc cgttcttcag
     2341 ccgctgcacg cccacgtgat cgatcggatg cttgaccggc tcgggggccg gaccgtcgat
     2401 cagcggccgc ttgaggtact cggtctcgag aacttctcgg aatctttcga caccccagtc
     2461 cttgatgagg aacttcagtc gtgccttggc ccgcagccgc cgatagccgt agtcgcgaaa
     2521 tatcgacgtc accgccgccc acacctcggg cacctcgtcc agcgggaccc aggcgccgac
     2581 ccgttgcccc agcatggggt tggtggatag gccaccgccg acccacaggt ccaggccggg
     2641 cccgtgctcg ggatggttga cgccgatgaa tgcgatgtcg ttgacctcgt gcaccacatc
     2701 ctgcaggccg gagatcgccg tcttgtattt gcgcggcagg tcggcgaagt cgggcttgcc
     2761 gatgtagcgg cgcacgatct cgtcgatcgc ccaggtggga tcgagcacct catcgagcga
     2821 ctcgccggcc agcggtgagc ccagaatgac gcgggggcag tcaccgcacg cctcggcggt
     2881 ctgcagtccg acctcgtcaa gccgccgcca gatttcgggg acgttctcca cctcgatcca
     2941 gtggtactgc acattctcgc gatcggagat gtcggcggtg tcgcgcgcga attctgtcga
     3001 gatctggccc agcgtgcgca gcgcggccgc ggagagcgcg ccgccgtcgc agcgcacccg
     3061 catcatgaag tatttggctt cgagcagctc aatgttctcg tcgccggtga aagtgccatc
     3121 gtagccctgc tcgcgttgcg tgtagaggcc ccaccagcgg aagcgcccgc gcaggtcgga
     3181 tttgtcgatg ctgtcgaagc cgtccttggc gtagacgttt tcgatgcgtt cccgcacgtc
     3241 gagcggagcg ccggcttgct tcatctc
//
LOCUS       DASSGQ010000388         8461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_1093030,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000388 DASSGQ010000000
VERSION     DASSGQ010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8461
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093030"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFI55_17130"
     CDS             complement(<1..352)
                     /locus_tag="VFI55_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075786.1"
                     /translation="MSADSQLAAVLGGRAVGLPRRSVIEAAWRSIGPGVEVFSGDDGG
                     PLSRTVKRIIDPLVLRLRANPQYSAPVVAPETAVAMHELIVASGAELRSAAAWFEILK
                     LERRRQRIRTGNAQE"
     gene            436..831
                     /locus_tag="VFI55_17135"
     CDS             436..831
                     /locus_tag="VFI55_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075787.1"
                     /translation="MKKLTVLLLGTEIAVLATAVVVVVVALLLVSRVGLGRRQVADGR
                     TVGVGTVRAIGNAGGAPAAGERQIFIEVVAVSGETFIGKLARCDRDQDVSMLRPGLVV
                     LVAFNPAAREELSLPDDVLAVHASWLASI"
     gene            complement(835..2205)
                     /locus_tag="VFI55_17140"
     CDS             complement(835..2205)
                     /locus_tag="VFI55_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HET7075788.1"
                     /translation="MTETDRLTLVLRFADVGVATYASLRVVGQPSRTVTWVVEQPILL
                     AALQELADALPDPHGSESGRDAIERALTRGPFAAPDTELTVAYILGVLLIGASGWQLL
                     TECLASPRAVLFISPSARLARVPWGLLAIPQSGPSTEELIRARNDAVTHAGPMPARIP
                     WQLADIGAHTEGYRLMELVDVLMTVPPNIVHSPRVPAGWDARRDGPPVLVLDPRVPGQ
                     RPDSALGSVLGRPSSQTPLARHFADLMQRRRVLPDVDDVVELFRRQDADRAWLANLLA
                     HTPSRLMYVGHASSAPREADHAGRADRAALHLACTATIPGDADAIGDHRPLTASDLMS
                     MRLPMPPRVALLACGSGGDYQFDEATGLVAATILGGAQLVTATLWSLPTAAAHRRFGS
                     AAEEPADPMADLVIAVDRAHAQDDAGCAVNRWQRAQLRLWRDGVLTASPLYWGALVTF
                     AVDGAR"
     gene            complement(2202..3953)
                     /locus_tag="VFI55_17145"
     CDS             complement(2202..3953)
                     /locus_tag="VFI55_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075789.1"
                     /translation="MSIPGELQNARRLAFAADEMRARDLLLSLIPQIEREDRDDLMLE
                     VLAQLGEIYLARGANDGVREAIRRIRDCLASYSAIAAGTMPHTAEQVRMSDVEMAHMI
                     CRYSRRAQFLQAGLAAAQGDHEAAAAALTMLCDNESGQDFADLIDEHAYLRTYGQILC
                     ATALCDDDLYVPSVSLWEQALYGIDRVRNESEFADHLLISAATAYGRFCVETGRLPEA
                     EPWLRRAEARARAKGWELATARTQLERAAARWSVGDHVATEQLVSAAYPVIARHARAH
                     DVSRCWLYLGLTRLAVGALEAADQCWEHAERHWRELGKPLHTHRILLQRSWIEIFRGR
                     FAAAVELIAQAREFLDSSPRSSWLQYARLDSHLGNVWRADALTDLGFDAAGEASDTWR
                     EAEARYAKALGVTHAEVGSPQYLRAMTKLEQAAELKVPAALAVDSVRYSIADADARSR
                     WATWVSAPMLAGAFAVAWEWDNTELLSELVEYHSARGTFSTEPHARNHRPEIGEWTST
                     ATSPVLLDTGDGPATALAAAGPSPHRGVSLTRLGPLPPLQMQPAAPPILSHYRALALQ
                     RYGREVTAGEPAWSTWP"
     gene            complement(4048..4545)
                     /locus_tag="VFI55_17150"
     CDS             complement(4048..4545)
                     /locus_tag="VFI55_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LpqH"
                     /protein_id="HET7075790.1"
                     /translation="MKEGNAVKRLLAGAIGTLGCASALVACSTGGHTASPASSTAPSV
                     ASGSGAQVKVGGSDLPGLNPASVTCVRTGGKIDIGSGSTGGAQQALAVVMTDANPPTV
                     DSLALVVDGNALSVSNMGAKVGSANVAVDGKTYTITGQAQGADMKNPMAGMVTKDFNI
                     KVTCG"
     gene            4666..5043
                     /locus_tag="VFI55_17155"
     CDS             4666..5043
                     /locus_tag="VFI55_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075791.1"
                     /translation="MASVKWLEEPEAHDFPAAADYLALLADADTVDRVTAKLKAATVT
                     HKKAKDILRAARLFLLPEDNAHVASDLAKIKKGQPLSPILLVRGDFTKGVPLQIADGY
                     HRVCASFHTDENTDIPVVIVPSS"
     gene            5058..5804
                     /locus_tag="VFI55_17160"
     CDS             5058..5804
                     /locus_tag="VFI55_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET7075792.1"
                     /translation="MIDLQAAGAVRVLTLSSGAVNALDVELLQELAGALRGLRDSGAP
                     ALVVTGAGRVFSAGVDLNRVVDGPAGYTDRLVPAISEAIGALFGYPGPTVAAINGAAI
                     AGGCVLACACDRRLMSPQARIGASEVRVGVPFPVAALEIMRYACGDHAEDVLLGGRVY
                     QGAEATANGLVHRVVTDDLIGAAVAEAADLDGIPVDAYRHTKAQLRAPALARMREGDS
                     VDAEGRRLWAADETRRGIAAFLEGLRRRDG"
     gene            complement(5801..6589)
                     /locus_tag="VFI55_17165"
     CDS             complement(5801..6589)
                     /locus_tag="VFI55_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075793.1"
                     /translation="MGRKVAIPWHASFSVAAGVLYFFFVLPRWPELMGDTTHSLGTAL
                     RIVTGVLIGLAALPVVFTQMRTRKPELGTPQLALTIRVWSIIGQALAGALIISTAVSE
                     IWLSLDVAGQWLFGIYGAAAAIGLLGIFAFYLSFVAELPPPPPKPIKPKKAKQPKKRG
                     LRRKQADEADETEDIDETEEADEADETEAEEPVKVAAEPEAEPEAATVEAPKAEHTEE
                     TEADEETEDTEDTRGGLRNRRPTGKGSHRLRRSRPAAGSRSTNN"
     gene            complement(6704..6792)
                     /locus_tag="VFI55_17170"
     tRNA            complement(6704..6792)
                     /locus_tag="VFI55_17170"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6756..6758),aa:Ser,seq:gga)
     gene            6880..6974
                     /gene="ffs"
                     /locus_tag="VFI55_17175"
     ncRNA           6880..6974
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFI55_17175"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            7144..8433
                     /locus_tag="VFI55_17180"
     CDS             7144..8433
                     /locus_tag="VFI55_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7075794.1"
                     /translation="MSFDSLSPVELAAAHTRNQQNYAELQAKKLALDLTRGKPSAAQL
                     DLSNQLLSLPGDDYRDGEGTDTRNYGGLHGLPELRAIFGELLGIPVPNLIAGNNSSLE
                     MMHDLVVFSMLYGGVDSRRPWKDEPSVKFLCPVPGYDRHFAITETMGVEMIPIPMLQD
                     GPDVDLIEELVAVDPAIKGMWSVPVFGNPTGVTYSWETVRRLVQMRTAAPDFRLFWDN
                     AYAVHTLTHDFVRQVDVLGLAAQAGNPNRPYVLASTSKITFAGSGVSFLGGSLGNIAW
                     YLQYAGKKSIGPDKVNQLRHLRFFGDADGVRLQMLRQQQILAPKFALAAEILDQRLSD
                     SKIASWTEPKGGYFISLDVLPGTARRTVALAKDAGIAVTEAGASFPYRKDPDDKNIRI
                     APTFPSLPSLRDAVDGLATCALLAASESLVSTASSVS"
BASE COUNT         1312 a         2855 c         2871 g         1423 t
ORIGIN      
        1 gttcctgggc gtttccggtg cgaatccgct gcctgcggcg ctccagtttg agaatctcga
       61 accatgctgc ggcagaacgt aattcggccc cgcttgcgac aatcagttca tgcatcgcga
      121 cggccgtctc gggggcgacg acgggggccg aatactgggg gttcgcgcgc agccgcagca
      181 ccaacgggtc gatgatccgt ttgacggtcc ggctcaacgg gccgccgtcg tcgccgctga
      241 acacctcgac accggggccg atcgaccgcc aggccgcctc gatcaccgac cggcgcggca
      301 ggcccaccgc ccgtccaccc aagacggccg ccaactgcga atcggcgctc atcagcacag
      361 gataggggca ttctggaaat atcggttcga gaaaagttgg gttcggtagc cggccggaag
      421 cgggagcctg gccgcatgaa gaagctgacc gtcctgctgt tgggcaccga aatcgcggtg
      481 ctggcaaccg ctgtggtcgt tgtggtggtt gccttgttgc tggtgtcccg agtcggcttg
      541 ggtcggcgtc aggttgccga cggccgtacc gtcggagtcg gcaccgtgcg cgcgatcggc
      601 aacgccgggg gcgcccccgc cgccggcgag cgtcagattt tcatcgaggt ggtggcggtg
      661 tccggcgaga cgttcatcgg caagctggcg cgttgtgacc gcgatcagga cgtgtcgatg
      721 ctgcgccccg gtctcgtggt gctggtggcg ttcaatcccg ctgcccgcga ggagctttcg
      781 ctgcccgacg acgtgctggc cgtccatgcg tcatggctgg cgtcgatctg agccctagcg
      841 cgcgccgtcc accgcgaaag tgaccagcgc tccccagtac agcgggctgg cggtgagtac
      901 gccgtcgcgc cagagccgca gctgggcgcg ctgccatcga ttcaccgcgc atccggcgtc
      961 gtcctgtgca tgtgcacgat ccactgcgat gaccagatcg gccatgggat cggcgggttc
     1021 ctcggctgcc gacccgaacc gccgatgagc cgcggcggta ggcagcgacc acaaggtggc
     1081 agtcaccaac tgggcgccgc ccaggatcgt cgcggccacc agaccggtcg cctcgtcgaa
     1141 ttgatagtcc ccgcccgatc cgcatgccaa cagggcgacc cgcggcggca tcggcaaccg
     1201 catcgacatc aggtccgacg cggtgagcgg gcggtggtca ccgatggcgt ctgcatctcc
     1261 gggaatggtt gccgtgcagg ccaggtggag cgccgcgcga tcggcgcggc cggcatgatc
     1321 ggcttcgcgt ggtgccgaac tcgcgtggcc gacgtacatg agcctgctcg gcgtatgggc
     1381 gagcaggttg gccagccagg cgcggtcggc atcttggcga cggaacagct cgaccacgtc
     1441 gtcgacgtcc ggcagcaccc ggcgacgttg catcaggtcg gcgaagtgcc gcgccagcgg
     1501 ggtctgcgac gagggcctgc cgagcaccga gccgagcgcg gagtccgggc gctgtcccgg
     1561 cacccgcggg tcgaggacca gtaccggcgg accgtcgcgc cgggcgtccc agccggccgg
     1621 cacccgcggc gagtggacga tgttgggcgg caccgtcatc agcacgtcta ccaattccat
     1681 cagccggtag ccctcggtat gggcgccgat gtcggcgagc tgccacggga ttcgggcggg
     1741 catcggaccg gcgtgggtga ccgcgtcgtt gcgggcacgt atcaactcct cggtgctcgg
     1801 cccggattgc ggtatcgcca gcagccccca gggcacccgc gccagccggg cgctcggcga
     1861 gatgaacagc accgcgcgcg gcgaggccag gcactccgtc agcagctgcc agccggacgc
     1921 gccgatcagc agcacaccca ggatgtaggc gaccgtcagt tcggtgtccg gcgccgcgaa
     1981 ggggccgcgg gtcaatgctc gctcgatggc gtcgcgcccg ctctcggacc cgtgcggatc
     2041 gggcaacgcg tccgcgagct cctgcagcgc cgcgagcaga atcggttgtt cgacaaccca
     2101 agtgacggtt ctcgacggct gccccaccac ccgcaagctg gcgtaggtgg cgacgcccac
     2161 gtcggcgaat cgcaggacca gggtcagccg gtccgtctcg gtcatggcca ggtcgaccag
     2221 gctggttcgc cggccgtgac ctcgcggccg tatcgctgca acgccagcgc gcgatagtga
     2281 ctcaggatcg gcggcgcagc cggctgcatc tgcaatggcg gcaaggggcc gagccgggtc
     2341 agcgacactc cgcgatgtgg cgatggcccg gccgcggcga gcgccgtggc ggggccatcg
     2401 ccggtgtcga gaagaaccgg cgacgtggcc gtggatgtcc attcgccgat ttctggccgg
     2461 tggtttcggg cgtgtggttc ggtgctgaaa gttcctcgcg cgctgtgata ctcgaccagc
     2521 tcactgagca actcggtgtt gtcccactcc caggcgacgg cgaaggcacc agccagcatg
     2581 ggcgccgaaa cccatgttgc ccagcgtgat cgcgcatcgg cgtcggcgat cgaataccgc
     2641 accgagtcga ccgcgagcgc ggccggcacc ttgagttcgg cggcctgttc gagcttcgtc
     2701 atggcgcgca ggtattgcgg gctaccgacc tcagcgtggg tgacgccgag cgccttggcg
     2761 taccgggcct cggcttcccg ccacgtgtcc gaagcctcac ccgcggcgtc gaaaccaagg
     2821 tccgtcaggg cgtcggcccg ccacacgttc cccaggtggc tgtcgaggcg cgcgtactgc
     2881 agccagctgc tgcgcggcga cgaatcgagg aattccctgg cttgggcgat cagctccacc
     2941 gcggccgcga accggcctcg aaatatctct atccagctgc gctgcagcag aattcgatgg
     3001 gtgtggagcg gctttcccaa ttctcgccag tggcgctcgg catgctccca gcactggtcg
     3061 gcggcctcca atgctccgac cgccaatcgg gtgaggccga gatacagcca gcagcgggac
     3121 acgtcgtgcg cccgggcgtg ccgagcgatg acggggtagg cggccgagac gagctgctcg
     3181 gtggccacgt ggtcgcccac cgaccagcgc gccgcggctc gctccaattg tgtcctcgcg
     3241 gtggccaatt cccatccctt cgcccgtgcc cgggcctccg cgcgacgcaa ccatggttcg
     3301 gcttccggca atcggccggt ctcgacgcag aaccggccgt atgcggtggc cgcggatatc
     3361 aggagatggt cggcgaactc gctttcgttc cggacgcggt ctatgccgta cagcgcttgc
     3421 tcccacaacg agaccgatgg cacgtacagg tcgtcgtcgc acagcgcggt cgcgcacaag
     3481 atttggccgt aggtgcgcag gtatgcgtgc tcgtcgatga gatcggcgaa gtcttgcccg
     3541 gattcgttgt cgcacaacat cgtcagcgcc gcagcggcgg cttcgtggtc gccttgcgcg
     3601 gcggccagtc ccgcctgcag aaactgtgcc cgtcgcgagt agcgacagat catgtgcgcc
     3661 atctcgacgt cggacatgcg cacctgttcg gcggtgtgcg gcatcgtgcc ggcggcgatc
     3721 gcggaatagc tggccaggca atcccggatg cgccggatcg cctcgcgcac tccgtcattc
     3781 gcgccgcgcg caaggtagat ctcaccaagc tgcgcaagaa cctccagcat gagatcgtct
     3841 cggtcttccc gctcgatctg cggaatcagg gacagcagca gatcccgggc ccgcatttcg
     3901 tcggcggcaa acgccaacct gcgggcgttc tgcaattcgc cggggatcga cacggctgca
     3961 tcataagtcc gccgtcgtgg acacggcgag ggcccggagc ggcggacgcg tgagtgaggg
     4021 cacgtccgcc gcccggagtc cgatcgatca accgcacgtc accttgatgt tgaagtcttt
     4081 ggtgaccatt ccggccatcg ggttcttcat gtcggcaccc tgcgcctgac cggtgatggt
     4141 gtaggtctta ccgtccaccg ccacgttggc ggagccgacc ttcgcgccca tgttgctcac
     4201 cgacagcgcg ttcccgtcga cgaccagcgc cagggaatcg accgtcgggg gattggcgtc
     4261 ggtcatcacc acggccagcg cctgctgcgc gccaccggtg gatccgctgc cgatgtcgat
     4321 cttgccgccc gtcctgacgc aggtcaccga ggcggggttc aggcccggca ggtcggaacc
     4381 gcccaccttg acttgcgcgc cgctgccgct ggcgacgctg ggcgccgtgc tcgatgcggg
     4441 gctggccgtg tgtccacctg tcgagcaggc caccagcgct gacgcacagc caagggtccc
     4501 gatcgctccc gcgagaagac gtttcactgc atttccttcc ttcatgcggc gccccattgg
     4561 cgtcgcgatg cacgcaagta cagaacgccc gcggcgctac cgatcccaat ttcctggttc
     4621 aggcggtagg ctgcgtcgac acggcccaac gaagggagac gcccgatggc cagcgttaag
     4681 tggctggaag agcctgaggc acatgacttt ccggcggcag ccgactatct cgctctgctc
     4741 gcggatgcgg ataccgtcga tagggtgacg gcaaagttga aagccgcaac cgtcacccac
     4801 aagaaggcca aggacattct ccgggccgcg cgcctgttcc tgttgcccga ggacaatgcc
     4861 cacgtcgcct cggacctggc caagatcaag aagggccaac cgttgtcgcc catcctgctg
     4921 gtgcgcggcg atttcaccaa gggtgttccg ctgcagatcg ccgacggcta ccaccgggtg
     4981 tgcgcgagct ttcacaccga cgagaacacc gatatcccgg tcgttatcgt gccgtcgagc
     5041 tgacgaccgg cggcatcgtg atcgatctcc aggccgcggg cgcggtgcgg gtgttaaccc
     5101 tttcctccgg ggccgtcaac gccctcgacg tcgagttgct gcaagagctg gccggcgcgc
     5161 ttcgcggcct gcgggactcc ggtgcccccg cactcgtcgt caccggtgcc ggccgggtat
     5221 tcagcgcggg agtcgatctc aaccgcgtgg tggatggccc tgccggctac accgatcggc
     5281 tggtcccggc gatctccgag gcgatcgggg cgctgttcgg ttatccgggg ccgacggtgg
     5341 ccgcgatcaa cggcgccgcc atcgccggcg gctgtgtgct ggcgtgcgcc tgcgaccgcc
     5401 ggctgatgag cccacaggcg cggatcgggg cgtccgaggt ccgcgttggg gtgccgtttc
     5461 cggtcgccgc gctcgagatc atgcgctacg cgtgcggaga ccacgccgag gacgtgctac
     5521 tcggtggccg ggtctaccag ggggccgagg ctaccgccaa cgggcttgtc caccgtgtcg
     5581 tcaccgacga tctcatcggg gcggcggtag cggaggccgc ggaccttgac ggcatcccgg
     5641 tcgacgccta tcgccatacg aaagcccagc tgcgagcccc cgcactggca cggatgcgcg
     5701 agggtgacag cgtcgacgcc gagggccgcc ggttgtgggc cgccgacgag acgcggcggg
     5761 gcatcgcggc attcctcgag ggtcttcgcc gccgcgacgg ctagttgttc gtcgagcgcg
     5821 aacccgccgc ggggcggctg cgccgaagcc gatgcgaccc tttcccggtc ggccgacggt
     5881 tgcgcaggcc accgcgtgtg tcttcggtgt cttcggtttc ttcgtcggct tcggtttctt
     5941 cggtgtgctc ggctttcggc gcctcaacgg tggcggcttc gggctccgcc tcgggctcgg
     6001 cggccacctt gaccggctct tcggcctcag tctcgtcggc ttcgtcggcc tcttcggtct
     6061 cgtcgatgtc ttcggtctcg tcggcctcgt cggcctgctt gcggcggagg cctcgcttct
     6121 taggctgctt ggctttcttc ggcttgattg gtttcggcgg tggcggcggc aactcggcga
     6181 cgaacgacag ataaaacgcg aagatcccga ggaggccgat cgcggccgcc gcgccgtaga
     6241 tcccgaacaa ccactggccg gcgacatcca ggctgagcca aatctcgctg accgcggtgc
     6301 tgatgatcag cgcgccggcc agcgcctggc cgatgatcga ccagaccctg atggtgagcg
     6361 ccagctgcgg cgttcctaac tccggctttc gggtccgcat ctgggtgaac acgacgggca
     6421 gcgctgccaa gccgatcagt acgcccgtca cgatccgaag cgcggtcccc agggaatggg
     6481 tggtatcgcc catcagctcg ggccagcggg gcagcacgaa aaagaagtag aggacgccgg
     6541 cagcgaccga aaacgacgcg tgccaaggta tggcgacctt gcgccccata cgcctcctca
     6601 tgctggtcct gagccttgtt ggtggtaagc cagcctaaag gccggtttaa ccgattccaa
     6661 gcaacgcccc tgctcggccg cgtcttgaac cgagtgcgtt cgttggcgga ggatgcggga
     6721 tttgaacccg cgagggctgt taacccaacc cgcgttccag gcgagcgcca taggccacta
     6781 ggcgaatcct ccgccgccat ggtagccgag tgggttgggt tgccaagacg actgctgccg
     6841 gttgcacgcg ggttgccatg ggccaggtac tacactcgcg atggaccccg cgcggcgtct
     6901 atcctgtgaa ctcccccagg gccggaaggc agcaagggtc aatgggctct gtcgggtgcg
     6961 cggggtcccc taggcttgat gagtggcgac ccgctgcgcc cggctccgcc gcgcttgcga
     7021 tcaccactag gctcgatgag tggcgacccg ctgcgcccgg ctccgccgcg cttgcgatca
     7081 ccactaggtt cggggccctg ccggccccgg aactcccagc gatcgacagc gaaagggccc
     7141 atggtgtcgt tcgattccct cagccctgtt gagctggccg cggcgcacac ccgcaaccag
     7201 cagaattacg cggagcttca ggccaagaag ctggccttgg atttgacccg gggcaaaccg
     7261 tcggccgcgc agctcgacct gtcgaaccag ctgttgagcc tgcccggaga cgactaccgc
     7321 gacggcgagg gcaccgacac ccgcaactac ggcggcctgc acggcctgcc cgagcttcgg
     7381 gccattttcg gggaattgct cggcatcccg gtgccgaact tgatcgcggg gaacaactcc
     7441 agcctggaga tgatgcacga cctggtcgtc ttctcgatgc tgtacggcgg cgtggattcg
     7501 cggcggccct ggaaggacga gccgagcgtc aagtttctct gccccgtccc cggctacgac
     7561 cggcactttg ccatcaccga gaccatgggc gtcgagatga tcccgatccc catgctgcag
     7621 gacggtcctg acgtcgacct gatcgaagag ctggtcgccg tcgaccccgc aatcaaaggg
     7681 atgtggtcgg tgccggtgtt cggcaacccc accggggtca cctactcctg ggaaacggtg
     7741 cggcggctcg tccagatgcg gacggcggcg cccgatttcc ggctgttctg ggacaacgcg
     7801 tacgcggtgc acacgctgac acacgacttc gtccgccagg tcgacgtgct tgggctggcg
     7861 gcccaagccg gcaacccgaa ccggccatac gttttggcgt ccacttccaa gatcaccttc
     7921 gccggctccg gtgtcagctt tctgggcgga tcgctgggca acatcgcctg gtatctgcaa
     7981 tacgcgggga agaagtcgat cggcccggac aaggtcaacc aactgcggca tttgcgcttc
     8041 ttcggcgatg ccgacggggt acgcctgcag atgctgcggc agcagcagat actggccccg
     8101 aaattcgcgc tcgcggccga gatcctggat cagcggctga gcgattccaa gatcgcctcc
     8161 tggaccgagc ccaagggcgg ctatttcatc agcctcgacg tgctgcccgg caccgcccgg
     8221 cggactgtcg ccctggccaa ggacgccggt atcgcggtga ccgaggcggg cgcgtcgttc
     8281 ccctaccgca aggatccgga cgacaagaac atcaggattg cgcccacgtt cccgtcgcta
     8341 ccgagcctgc gtgatgcggt cgacgggttg gcgacctgcg cgctgctggc ggcgtccgag
     8401 tcgctggtct ccaccgcgtc cagtgtgagc tgagggcggg aaatccgccg gcgaaatcgc
     8461 g
//
LOCUS       DASSGQ010000389        15970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U1037 k141_182213,
            whole genome shotgun sequence.
ACCESSION   DASSGQ010000389 DASSGQ010000000
VERSION     DASSGQ010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963202
            Sequence Read Archive: SRR25158204
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:35:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,454
            CDSs (total)                      :: 3,419
            Genes (coding)                    :: 3,356
            CDSs (with protein)               :: 3,356
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 11 of 63
            Pseudo Genes (multiple problems)  :: 12 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15970
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182213"
                     /isolate="SMAG_U1037"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32305556 N 113.6236111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            369..771
                     /locus_tag="VFI55_17185"
                     /pseudo
     CDS             369..771
                     /locus_tag="VFI55_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167375.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            917..1765
                     /locus_tag="VFI55_17190"
     CDS             917..1765
                     /locus_tag="VFI55_17190"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HET7075795.1"
                     /translation="MAFERHRLPTLIGQGRTGKVIAAAAAAIAFAPVSVLATTPAVAQ
                     AAPWAQATLPGPPPTMTIPTVAALEDSGRPWWSMGLHYGVLVIGVCFVAWMIHRFAKT
                     WRADLTAAPAPAPARRSLGRGGFEGVLRARKKTALVEAGHRCAIPTCRHPTTEIAHIV
                     PPSEGHDDSFENLIALCPKCQKKEIDAQSMRMYKRNLAILNSRYSDFERRVFDQIAET
                     DQRSFTVEAGFEIPLFHAVNDGLLRRVELAPVGIQRGEATHYTYEVTDEGLDFVNRYV
                     CGEDIS"
     gene            complement(1888..3276)
                     /locus_tag="VFI55_17195"
     CDS             complement(1888..3276)
                     /locus_tag="VFI55_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7075796.1"
                     /translation="MVVAVMVSMVAFLDSTVVNLALPATERDLGGGMCLQQWVVDGYL
                     LALAATILPGGSVSDQFGRVSVMRLGLVAFGVGSALAATAASPAMLIAGRLIQGFGAA
                     FLVPGSLALINSVFDPAHRTAAIGSWTAWTGTAFAVGPLLGGLAVDLLSWRWIYALSA
                     VPMVIGFALTFWLPPVPGPARRARVDIPGAALSAAGLGATVYALIESGKRGWAAPLVA
                     PSLVVGITALLAFVVWERRAPHPLVPPQLFRVRNFAGANLTTSFVYGALTMSSLAISL
                     YTQEVAGYCATVAGLVTLPSPVISFLFAKRVGHLAARIGPRNFLMAGPALAGFGLLLI
                     RPSAHAFDIVTDLLPGMIVLAIGMVVTNTPLTAVSLSSVDAAHSGIAAAMQNAVGRTS
                     ALTSVASIGLIAAGTLTDASFTRLLQVAAVLFFIGAVISRVTVTNPARPTAPTDARAT
                     PAVADGVAGRAA"
     gene            complement(3813..3884)
                     /locus_tag="VFI55_17200"
     tRNA            complement(3813..3884)
                     /locus_tag="VFI55_17200"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3850..3852),aa:Val,seq:cac)
     gene            4061..4133
                     /locus_tag="VFI55_17205"
     tRNA            4061..4133
                     /locus_tag="VFI55_17205"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4094..4096,aa:Gly,seq:gcc)
     gene            4162..4235
                     /locus_tag="VFI55_17210"
     tRNA            4162..4235
                     /locus_tag="VFI55_17210"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4194..4196,aa:Cys,seq:gca)
     gene            4250..4324
                     /locus_tag="VFI55_17215"
     tRNA            4250..4324
                     /locus_tag="VFI55_17215"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4282..4284,aa:Val,seq:gac)
     gene            5055..6953
                     /locus_tag="VFI55_17220"
     CDS             5055..6953
                     /locus_tag="VFI55_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7075797.1"
                     /translation="MPRDLGFVTSVAGQLMEANASTANSVCEQVLARLVDKFDADSGY
                     LRHNDQTIGASRLIAEWPPRPNRPHTDLLPIVDFTSADDVFEPCAHGKEPLVIQPPEP
                     LVIRPLASTNYAYRALRRQITGRRRIATPSLAAAPLVSKGETTGVLGFVNLRGRKWKQ
                     QEVRTLGAVATLFAQLQVRIAAEERLYYLADHDDLTGLHNRRSVVALLSERLAAGRPG
                     PVAVLYLDLDRLKSINDYFGHAAGDFFLRVFAERLEASMRELGTIGRIGGDEFLVFPD
                     RAMLTGTAESLAHRLQTTLRGLLKIGDDVITPSVSIGVGVGRPGRDSAADVLRRADEA
                     ALTAKRAGGNQVAVCTTDISRNVVFRNDIELHLQAGIDNDALLLHYLPEVDLLTRVIL
                     GAEALVRWQHPTRGLLLPDSFIGVAESTNLAAGLGRWVMRSACAELSRWRSNGVGQGA
                     VMRINVSPVQLITRGFVRSVADTIDEFGIDAGSVCLEITERAVVHDIETTRKTLSELK
                     DVGVQIAIDDFGTGYAVLSHLKSLPVDMLKIDTGFVRDLGNNTSDLAIVRAIIGLAEA
                     FGLQLVAEGVETPAAVQTLIEHGCHRAQGFLFSRPVAGDAMESLLSARRMPMTWLTDD
                     ETRSAAAS"
     gene            7612..7926
                     /locus_tag="VFI55_17225"
     CDS             7612..7926
                     /locus_tag="VFI55_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075798.1"
                     /translation="MIPPHNDSIWPDDTKRAAVTLALAAATTSGMQFDDVIEAIEEAL
                     NSGRPLQFVMELIRLLTAGLGEEFSNSGTATAYQQLAAQCQAAQDDMRSRGEWRNDDR
                     PD"
     gene            complement(7958..8263)
                     /locus_tag="VFI55_17230"
     CDS             complement(7958..8263)
                     /locus_tag="VFI55_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075799.1"
                     /translation="MRHNTISGHYGAHHTVAPLFWVVAGIIVVVAFGDALAVLAIVAA
                     IAATAWLLYQEFRLERRAVTGNDTTTAYVTNLRPALTGRRDLTKTSAHASWRGRSAA"
     gene            complement(8447..9931)
                     /locus_tag="VFI55_17235"
     CDS             complement(8447..9931)
                     /locus_tag="VFI55_17235"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7075800.1"
                     /translation="MRFESVFDAVIVGAGFGGIGAAIQLKRLGYDDFVILDREADLGG
                     TWHVNRYPGVAVDIPSTTYAYWFEPNPYWSRLFAPGAELKRYAEHVADKYDVRRHMRF
                     NQIVQGARWVEDAHVWQVSLAGGQTLTTRILITATGFLSQPRIPDIPGLDSFDGKMIH
                     TAAWDDSYRFAGRRVGLIGTGATAVQLIPELAKRVADLTVYQRTPVHTVPKVDFAIPA
                     GVQRMFARMPLAQRLFRWITDTFFELITVTALRYRQFRRLGVDGGDLAAIHRFVSVRD
                     AELRRQLTPDYNFGCKRPTWSNDYYGVFTKPHVHLQTNPIERVEPDGVVTADGRKDVI
                     DTLVLATGFDLWDANFPAIEVIGRRGRNLGKWWRESRFQAYQGVSVPWFPNFLNLVSP
                     YAFSGLSFFNTIEYQMRHMDRLLGEVKRRGATTFEVTEEANARFLDRMTQLLVDSVFR
                     WGDCATSRSYYFNRSGEATLLRPTLTRNAVREASTFPLHDYTIA"
     gene            complement(9955..10953)
                     /locus_tag="VFI55_17240"
     CDS             complement(9955..10953)
                     /locus_tag="VFI55_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7075801.1"
                     /translation="MDISCAFATSSDTPAHVELAEALGYRRAWLYDSPALYPDVWMIL
                     TRCAERTSRIGLGPGVLVPSLRHPMVNAAAIAQLVDQAPGRVVVAIGSGLTGRMALGQ
                     RPMPWRKVAEYVRCVKALLAGETAEWEGAKIRMLHLPGFGAQRPVEVPILIGAGGPKG
                     LAVAAELGDGVFSAGIPQPDAAKVADWQALLCLGTVLDEGEEVMSPRAVDAAGPGAVV
                     IYHTVYEHGGAAAVDHLPGGRSWREAIEACPEDERHLAIHAGHLVQANPHDEPHVADL
                     LPFASSRAMTGTAEQITEKIAGFAAQGVTEIVYQPAGSDIERELRVFASAAGLGNS"
     gene            complement(10992..12167)
                     /locus_tag="VFI55_17245"
     CDS             complement(10992..12167)
                     /locus_tag="VFI55_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7075802.1"
                     /translation="MKFVLAGYGSRGDVEPCATVGRELLRRGHSVCMAVSPDMTGFVE
                     SAGLTAIAYGPDSRKQMKTAADFISTAANPISALPAALQDLTEVWTAKTTTLTSLAEE
                     ADLLLAGINEQSLAATVAEYCRIPLAALHFFPACILPAGIHSGMTNIIADAQRSALGL
                     PEATESAAASLEIQAYDELCLPGPAAEWVGTDGRRPFVGALTLEMATDGDDEVLSWIA
                     GGAPPICFGWGSTPIASPAHTIAMIGAACARLGERALICGGPNDLTNLPRFDDVKVVV
                     AVNHATVLPACRAVVHHGGAGTTAAGLRAGIPALILWLWLDQPVWAAGLERLGVGSGR
                     PFAATTEESLVADLGCIRTPEYVARAREVAAEMTKPAESVGRAADLLEDAARLGRTD"
     gene            12265..13233
                     /locus_tag="VFI55_17250"
     CDS             12265..13233
                     /locus_tag="VFI55_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET7075803.1"
                     /translation="MHVINGVRDPAASFPLDTAADDTAERRQANRALAVSAVGLALTG
                     LVELAIALISGSVALLGDALHNLSDVSTSALVFIGFRASRKVATERYPYGQERAEDLA
                     GVGVAVVIWGSAVVAGFESVTKLLRHGGTGHLGWGIAAAAAGIAGNQLVARYKLVVGR
                     RIRSATMVADAKHSWLDALSSAGAMLGLIGVALGWGWADAVAGIVVTGFICHVGWEVT
                     ADIAHRLLDGVDPEIITTAEAVATTVPGVLHAHARARWTGRTLRVEVEGFLAHDTPLA
                     EADRIGRNVASTLAPRIPEMHSFSWAARAASHESLCAGNDSTVSLS"
     gene            complement(13261..13899)
                     /locus_tag="VFI55_17255"
     CDS             complement(13261..13899)
                     /locus_tag="VFI55_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075804.1"
                     /translation="MKVSVAAAGWVTSTLVVAVGVAGCGGDKSSGPSSATSTTSTAAS
                     SSAASSSGTPTPSSGAAQPSDYSGLLIKPADIVVPGDTFTLMQTLPVPNPAGVEGVFM
                     NQGGTRKVDDTIYVYPDAGAAAQALDQSAKGIQELSVKAPPTPADVGTGGQMAVGPSP
                     DGAKAKGIVMFTEGKVFTVLEFESPQNDPVQPDFVLDLGRKQDAAIKSAPPA"
     gene            complement(14019..14957)
                     /locus_tag="VFI55_17260"
     CDS             complement(14019..14957)
                     /locus_tag="VFI55_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET7075805.1"
                     /translation="MRVDPGPLTWAALVQTWHLDLASAAVIVLVAGGYAWCYRRARSA
                     EQPVRPAQAACFGVGIVLWVLATASVIGVYAYALFWMRALQVLMLLYVVPFFIAVGKP
                     VTVVRDALGPAGRERIDRWLATPFARVLAHPLTTSLAMLGTPWLLYLTPWYTASLENE
                     WIGAPTRILLVAIGVSYFYARLQADPVPRKYPQLISLLITVAEALGDGVLGLVVWQGS
                     LIGAGYYSELHRSWGPDQRLDQTIGAGVLWILGDLVGWPFVLLLMRALSRDEKAHAVA
                     VDAELDSAAVTRTETAAPSGLWWENDPELRERFRRR"
     gene            15054..15668
                     /locus_tag="VFI55_17265"
     CDS             15054..15668
                     /locus_tag="VFI55_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7075806.1"
                     /translation="MAAIFIVLDVFVGILALLGVIALGSRLGARRRTPGVKGVLLVSE
                     RLAPRRKNVARFQTRLRMVGPVVRYEVALDLEADGQRFETSSAQPATRPSMGCDDEEL
                     SWSFEVPEDDVDRLWVIASWVEGRGHDLRSAALTQQLSTSETYEWKWYLRSLGGWQKR
                     RTATTPTATEPGRGPLELGHAPDHRRESPPAAEPDTTEEEPGHE"
     gene            complement(15700..>15970)
                     /locus_tag="VFI55_17270"
     CDS             complement(15700..>15970)
                     /locus_tag="VFI55_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HET7075807.1"
                     /translation="RADSNDDAFLASLQAAGITYPDPGRVIAAGRWVCQAVDQGTQMV
                     DIVKTVQAQNSGLHGDNAAKFTAIAANVYCPTALTTNGGKPGGDS"
BASE COUNT         2874 a         5238 c         5196 g         2662 t
ORIGIN      
        1 catcctcaga atcctcgggc cgtggttctg gtggccaaca ctggtgcgcg cccgcccagt
       61 accgcagcac taaaggttgc ggtcccacgc cggacgattc tttccgattg cctcagtcgg
      121 tggtgtgaac gatcagtaga tcggtcttgg cgctgcggga gaccacccgc aggtatcagc
      181 cggggtcgtt gccgctgatg tgagaagtat cccttagtag ccgctagcgt tccttcgctg
      241 agcagggtac tctcttctaa atagtgaacg taaccgttta caatctcgca aggaccggag
      301 ccaccatgta caccgcaaca tcgcctgctg atgaatccac acatcggttg gaaaggccgc
      361 tcgacgagcg gcgactgcgc ttgacgccga tggcaggcgg gacgatcgcg ctggtcgcgg
      421 ggatcgcctt cttgacgatc accctgccgg cgaacgacgt agcgctggga ttcgatgtct
      481 tgcttgccgc attcatggcc atcaccgcgg tgctcgtttt cgttcgtcgg cagttggtgc
      541 cgccgaccgc gttcacgacc ggtgtgttgc tgatctgcga tgcgtggttc gacgtgatga
      601 cggccggccc ccacgacctg tgggcatccg cgctgactgc gacgctcgtc gagctgcccg
      661 tcgcgatcat gctgatcgcc atcgcgctgc gcatgtcgcg gctgaaccgt gacgcggttg
      721 tggctgctcg atcccgggat gccgccctgg cggccacctc tgctgccgta gaccacgcct
      781 acgcctgatc ggcgggacgt gtcggtgccg cacctggtgc cgctgccgtc caccgtcgcc
      841 ccggttggag tattttgagc tagtgggtcg ctagcatgtc cgaccaggca gggcgtggga
      901 aaggccaaag ggcaagatgg cgttcgagcg tcaccggctg ccgacgctga tcggccaggg
      961 cagaaccggc aaggttatcg cggcggctgc agccgccata gcgttcgcgc cggtctcggt
     1021 gctagccacc acacccgctg tcgcacaggc cgctccatgg gcgcaggcca cgctgccagg
     1081 tcccccgccc acgatgacga ttccgaccgt ggccgctctg gaggactccg gccgtccctg
     1141 gtggagcatg gggctgcact acggggtgtt ggtgatagga gtgtgctttg tcgcctggat
     1201 gatccatcgc ttcgcgaaaa cgtggagggc ggatctgaca gccgcgccgg cgccggcgcc
     1261 ggcccggcgg agtctcggcc gtggtgggtt cgaaggcgtg ctgagagccc ggaaaaaaac
     1321 ggcgttggtc gaggccggcc atagatgcgc catccccacc tgtcggcacc caacgaccga
     1381 gatagcccac atcgtgcccc cgtcagaggg ccatgacgac tcgttcgaga acctcattgc
     1441 gttatgcccg aaatgtcaaa agaaagaaat cgacgcgcag tcaatgcgca tgtataagcg
     1501 caatctcgcg atcttgaaca gccgctactc tgatttcgag cgccgggtgt tcgaccagat
     1561 tgccgagacc gaccagagat ccttcactgt cgaggcgggt ttcgagatcc ctctcttcca
     1621 cgcggtcaac gacgggcttt tgagacgagt tgaactagcg ccggtaggga tccagcgggg
     1681 tgaagcaacc cattacacgt acgaggtcac cgatgagggc ctggacttcg ttaaccgata
     1741 cgtctgtggt gaagacatat cgtgaggtcc cggcgttgaa atacaacgca tttcgcgaaa
     1801 ccgcgcgttt ctgaaatctg ttgtctcgca acaagattgt tttcaaacca ctgcgggtga
     1861 agaacggcta gcggagcgcc cgggccgcta cgccgccctg ccggccacac cgtccgccac
     1921 tgccggagta gcacgggcat ccgtcggcgc ggtgggccgg gccgggttgg tgacggtgac
     1981 gcggctgatc acggcgccga tgaagaacaa gaccgcggca acttgcagca gccgcgtgaa
     2041 gctggcatcg gtgagagtgc cggcggcgat cagtcctatg gacgccacgg aggtgagcgc
     2101 agacgtccgc cccaccgcgt tttgcatcgc ggccgcgatc ccgctgtgcg cggcatcgac
     2161 ggacgacagg ctcaccgcgg tcagcggcgt gttggtgacg accatgccaa tggcgagcac
     2221 gatcatcccc ggcagcaggt ctgtcacgat gtcgaatgcg tgcgcggagg ggcggatgag
     2281 tagcagccca aatccggcca gcgccggacc cgccatcagg aaatttcgtg gtccgatccg
     2341 cgcagccaag tggccgacgc gcttggcgaa cagaaacgat atgacggggc tgggcaaggt
     2401 gaccagaccc gcgacggtcg cacagtagcc ggcgacttcc tgtgtgtaca gggagatggc
     2461 aagcgagctc atcgtcaggg cgccgtagac gaatgacgtg gtcagattgg cgccggcgaa
     2521 gttgcggacc ctgaacagct gcggcggcac caaggggtgc ggggcgcggc gctcccagac
     2581 cacgaacgcc agcaatgctg tgatcccgac gaccagcgac ggcgcgacga gaggggcggc
     2641 ccatccgcgc ttccccgatt cgatgagtgc gtacaccgtg gcgccgagtc ccgccgcgga
     2701 caacgccgca ccagggatgt cgacccgcgc gcgtcgagcc ggcccaggta ccgggggaag
     2761 ccaaaacgtc aaggcgaagc cgatgaccat cggcaccgcc gacaaggcgt agatccaacg
     2821 ccagctcaat aggtccacgg ccagcccgcc cagcagcgga cccacagcga aggctgtgcc
     2881 cgtccacgcg gtccaggacc cgattgcggc agttcggtgc gcggggtcga acacggagtt
     2941 gatcagcgcc agcgagccgg gtaccaaaaa cgccgccccg aaaccctgga tgaggcgccc
     3001 ggcgatcagc atcgccggcg aagcggcggt ggccgccaac gccgagccga ccccgaaggc
     3061 caccagcccg agacgcatga ccgacactcg gccgaactga tcggagacag aaccgcccgg
     3121 caaaatcgtg gccgcgagag ccagcaggta accgtcaacg acccactgct gcaagcacat
     3181 gccgccgccg aggtcgcgtt cggtggccgg cagcgccagg ttcaccacgg tgctgtccag
     3241 gaatgcgacc atcgagacca tgaccgccac caccatgacg cggcttgtcg gcgccgcatc
     3301 gcggagttgg gagggccgac ctaccgggcg cgcggatgcg ggcaaaccga tccccgtagc
     3361 gaggcgtgtc gtcgtcatgg cagcgtcaca gcgtggttgt ggcgccggcg ccacaccttc
     3421 gcgagcgggt cgattcccct tcgaggctct ctcaccgtgg tcctccaaca acaatttcga
     3481 tagacccgcc cgctgcgtgc ccgcaattga atagggccgg gggttacgcg cgacccgacg
     3541 tgccgaaagc tgggcactgg tcgggcgggc aggtgcggcg cgcatctgtg attcgagcac
     3601 ggccctgacg gaattcgacc ttcgcagcac cgggatacgg ccgcctccgg cagcttgacg
     3661 cggcgcggct gtcgcctgga tcgtgtggcg gatgcgttcg acggcgtcgg ccactatcgg
     3721 aaagcgccgg ctggacggcg gacagcgtca ggcgcttgtg gcgatggatc gacgttcggg
     3781 cgtcgaaaag cgtatgactg gactgatggg ggtggtcccg gctgggatcg aaccagcgac
     3841 cttccgcgtg tgaagcggac gctcttccac tgagccacgg gaccggcgcc gaggggcgaa
     3901 cgacgtcgaa gactagcatg gcccggacgt cgcaggacgc gccgcgtgcg tcgcgagtgc
     3961 ttgctagcct aggtttgcaa cttaccaaga gatttgtgtg ggcccgctcg ggtggactat
     4021 cgtcgtcctt cgcaccgggc gacgatctcg tccgttgcgc gcggatgtag cgcagttggt
     4081 agcgcatcac cttgccaagg tgagggtcgc gggttcgaat cccgtcatcc gctcgaaggt
     4141 gcaagtggca tcaatcccag cggtggagtg gccgagtggt gaggcaacgg cctgcaaagc
     4201 cgtgcacacg ggttcgattc ccgtctccac ctccaagttc gaactcccag cgcgattagc
     4261 tcagcgggag agcgcttccc tgacacggaa gaggtcactg gttcaatccc agtatcgcgc
     4321 accaaagttt gcgcaggtaa agcacggttt atgacctgtc cgtcgacagg ttgcaaccga
     4381 tccacccgcg cccgggctcc cccaagcacg accaaccgcc gtgtcttttg ccggcggaga
     4441 cagggtttct cggctgtcaa acagcatccg cgaggtcgtc gccgtcgcga ctaagtgccg
     4501 cgcgtgaggc gtcgggccgc atccatcgcc agagccaacc aacgatccac ttggcgaggg
     4561 ctttgacgat ttccgtcaca aatcatttac cccaccaccg caataccgcg gctggtcgca
     4621 tttttggagc ctgatcggtc cgaatagggc acgtcgcagc tgttgctgcg accgtcaccg
     4681 ttcgcgctac ggtggcagtg ctcgccctga gggctcacct ggatcgtctt agaacaaatg
     4741 tcttcagttt ctcgaatatt gttgccgaac ttgagtttca caaccggcgc tcgccgaggg
     4801 gccaagcgat cgcgcggtga tgggactata gtcactcctg ccggcgtggc gaaggtccca
     4861 gcgctgaaac tcgcgttcgc cacccgcgca aaagggttgg tctccgcgtc attcgaacaa
     4921 cggcaaccac ctgaatgttc gaggagccca acgatgaccg agacgcagcg caagaccggg
     4981 ccggcatggg ccgaattctg tgatgatgcg ctcgaatcgg ttgcgatcgt ccgcaccgct
     5041 gcaatcgagt caccgtgccg cgcgacctag ggtttgtcac ctccgttgcc ggtcaactga
     5101 tggaggcaaa tgcctccact gcgaacagtg tgtgcgaaca ggtattggcg aggctcgtcg
     5161 acaagttcga cgcggacagc ggctacttgc gccacaacga ccaaaccatc ggggcctcga
     5221 ggttgatcgc ggaatggcca ccgcgaccca accgtcccca cacggatctc ctgccaattg
     5281 tggacttcac cagcgctgac gacgtcttcg agccgtgcgc ccatggcaaa gaaccgctgg
     5341 tgattcagcc cccagaaccg ctggtgatta gaccgctggc ctcgacaaac tatgcctaca
     5401 gggctttgcg gcgccagatc acgggaaggc gccgcatcgc cacgccctcg ctcgccgccg
     5461 caccgctggt ttcaaaaggg gagacgaccg gtgtgcttgg cttcgttaac ttgcgtggca
     5521 ggaagtggaa gcagcaagag gtgcgcacac tcggggcggt cgccacttta tttgcacagc
     5581 tccaggtccg cattgccgcc gaagaaaggc tttactacct ggctgatcac gacgatttga
     5641 ccggtctgca caatcgtcga tcggttgttg cgctgctttc cgaaagactc gcggcgggac
     5701 gccccggacc cgtcgcggtc ttgtatctcg acctcgaccg actgaagtcg attaatgatt
     5761 acttcggcca cgccgcaggc gattttttcc tccgggtatt cgccgaacga cttgaggcga
     5821 gcatgcggga gctgggcaca atcggtcgga tcggcgggga cgagttcctg gttttcccgg
     5881 accgggcgat gctaaccgga accgcggaat cactcgcgca ccggcttcag acgacgttgc
     5941 gcggtctcct gaagatcggt gacgatgtga tcacaccctc cgtcagcatt ggggtggggg
     6001 tgggcaggcc gggccgcgac agtgccgcgg acgtactccg gcgtgccgac gaggctgccc
     6061 tgacggccaa gcgcgcgggc ggcaaccagg tcgccgtgtg caccactgac atctcccgca
     6121 acgtcgtctt ccgcaacgac atcgaactcc atctgcaagc gggcatcgac aacgacgctc
     6181 tgctgctgca ttacctaccc gaagtcgacc tgctgacccg cgtgatccta ggggccgagg
     6241 cgctcgtccg ttggcagcac ccgacgcgcg ggctgctgct gccggattcg ttcatcggtg
     6301 ttgccgaatc gaccaacctc gccgctgggt taggccgatg ggtgatgcga agtgcctgcg
     6361 ccgaattgag ccggtggcga tccaacgggg tggggcaagg cgcggtaatg cgcatcaacg
     6421 tgtcgcccgt ccaactgatc acccgcggct tcgttcgtag tgttgccgac accattgacg
     6481 agttcggcat cgacgctgga tcggtatgcc tcgaaatcac cgagcgggcc gttgtgcacg
     6541 acatcgaaac cactcgcaaa acgctgtcgg agctcaagga cgtcggggtc cagattgcca
     6601 tcgacgactt cggcaccggc tatgcggtcc tatcgcacct gaagtccctt ccggtcgaca
     6661 tgctcaagat cgacaccggg ttcgtccgcg acttgggcaa caacaccagc gatctggcca
     6721 tcgttcgcgc catcatcggg ctcgccgagg cctttggtct gcaactggtc gccgaaggcg
     6781 tcgagacgcc ggctgccgta cagactttga tagaacacgg atgtcaccgc gcgcagggtt
     6841 tcctgttctc gcgccccgtg gcgggcgacg ccatggagtc attgctgtcc gcgcgccgga
     6901 tgccgatgac ctggctcact gacgacgaga cgcggagcgc ggccgcaagc tagggccgcc
     6961 caacagcgat gcccgtgccg gaaggctcac ctaccgtccc tcacattctc gaggtcgatg
     7021 tgccagatcc agttaccgaa gagcggtcgg tcaaaggcaa gcgcgccgag cggaccgatg
     7081 acgaagatag agagcaacgt ccatatcatt tcagccatta gtgactcccc tccgacaccg
     7141 atagcttcga attctgattg gtgagacggt tcataccttc gaatggttca ccgccccagc
     7201 ggcgttcagt agaggactcg atgttgtggg gggcacaaag cttcgttgtg gggcgccggc
     7261 ggtcccgcaa aaggcggcga cgatcaacgc tccggcgact gtcgataaag atatgccgca
     7321 tctcttgcct cgctctgtgc ctgacccacc gcgtgacgcg atagccgtta acttgtgaaa
     7381 cacctgagcg accggagggc tgcccaatga ggcgcgatcg tgaccgattt cactgggcgg
     7441 taagaggctg aagtgtcggc ggcggatttg ggggaattgc cctccgcgca tccgcgacgg
     7501 tctagtgtca ggcgcgtggc gggtagcccg cgagttgtcg aacgacgtgc cgcgcccggc
     7561 ccaaactaag cccggcccac catctttggc ggcgccgaaa ggaaattgga gatgataccg
     7621 ccgcacaacg attcgatctg gcccgacgac accaaacgcg cggccgtgac actcgcactt
     7681 gctgcggcca ccacgagcgg catgcagttc gacgacgtga tcgaagcgat cgaggaagcg
     7741 ctgaacagcg gacggcccct gcagttcgtg atggagttga tccggctgct caccgccggg
     7801 ctgggcgaag agttcagcaa ttccggcacc gcgaccgcct atcagcagct ggccgcacaa
     7861 tgccaagcgg ctcaagacga tatgcgttca cgcggtgaat ggaggaacga cgacaggcct
     7921 gattagcggc gcgtgcctaa agccaggcgc taccggccta tgcggcgctg cggccgcgcc
     7981 aggacgcgtg cgccgaggtc ttcgtcagat cgcgtcgacc ggtaagtgcc gggcgaagat
     8041 ttgtcacgta cgcggtggtc gtgtcgtttc cggtgaccgc gcggcgctcg agcctgaatt
     8101 cctggtagag caaccacgcg gtggccgcaa tcgcggccac gatcgccaac acagccaaag
     8161 cgtcgccgaa agccaccacc acgatgatgc ccgcgacgac ccagaacaag ggcgccactg
     8221 tgtggtgtgc tccatagtgc ccgctgatcg tgttgtgccg catatctgcc tcgctcgaaa
     8281 tcgaagtcgg cgcatctgat gcgcaactac gtgctacaac gcctgagcga tgccagggct
     8341 gcccaatctc gcgtggtggt gacgcatgtc actggggcgc atcaacaacg ggcacgtccg
     8401 gcgccggcca attgcgggtg tgccggcgcg acgagcctgt cggcacctac gcgatcgtgt
     8461 agtcgtgcag cgggaaggtc gacgcttcgc gcaccgcatt tcgtgtcaac gtcggccgca
     8521 acagcgttgc ttcaccactg cggttgaagt aatacgaccg cgacgtcgca cagtcgcccc
     8581 aacgaaacac ggagtcgacc agcagctgtg tcatccgatc gagaaatcgg gcattggcct
     8641 cttcggtcac ctcgaaagtt gttgcacccc tgcgcttgac ctcgccgagc agccgatcca
     8701 tatgtcgcat ctgatactcg atggtgttga agaaggagag cccggagaaa gcatatggac
     8761 tgaccaggtt caaaaaattg gggaaccagg gcacggacac gccctggtag gcctgaaacc
     8821 tgctctcgcg ccaccatttt cccaagttac ggccacggcg gccgatcacc tcgatggccg
     8881 ggaaattggc gtcccaaagg tcaaaaccgg tagccagcac gagcgtgtcg atgacgtcct
     8941 tgcggccgtc ggcggtgacg acaccgtccg gctcgacccg ctcgatcggg tttgtctgca
     9001 ggtgcacgtg cggtttggtg aacactccgt agtaatcgtt agaccaggtg ggccgcttgc
     9061 aaccgaagtt gtagtccggt gtcaattggc ggcgcagctc cgcatcgcgg accgacacga
     9121 accggtggat cgcggccagg tcgccgccgt cgacgccgag ccggcggaac tgtcgatagc
     9181 gcagcgcggt taccgtaatc agctcgaaaa aggtgtcggt gatccagcgg aacaggcgct
     9241 gcgccaaagg cattcgggca aacatccgtt gcacccccgc agggatcgcg aaatcaacct
     9301 tgggcaccgt gtggaccggg gtgcgttggt agacggtgag atcggcgacc cgtttggcca
     9361 gctcaggaat gagttgcacc gcggtcgccc cggtcccgat cagtccgacc cggcgccccg
     9421 caaagcggta gctgtcgtcc caggcggcgg tgtggatcat cttgccgtcg aagctgtcga
     9481 gcccggggat gtccggaatg cgcggttgcg acaggaagcc ggtcgcggtg atcagtatgc
     9541 gggtagtcag ggtttggccg ccggccagcg atacctgcca cacgtgggcg tcctcaaccc
     9601 agcgggcgcc ttgaacgatc tgattgaacc gcatgtggcg gcgcacgtcg tacttgtcgg
     9661 cgacgtgctc ggcgtagcgt ttgagctcgg cgcccggcgc gaacaaccgt gaccagtacg
     9721 ggttgggctc gaaccagtac gcataggtgg tcgacgggat atcaacggcc acacccggat
     9781 agcggttcac atgccaggtg ccgcccaggt cagcctcccg gtcgaggatg acgaagtcgt
     9841 cgtacccgag gcgcttgagc tggatcgcag cgccgatgcc gccgaagccg gcccccacaa
     9901 tgactgcgtc gaagaccgat tcgaaacgca cggtattcac aaatcactcc gtgctcacga
     9961 gttacccaga cctgcggcgg acgcgaatac gcgcagttcc cgctcgatgt cggatccggc
    10021 gggttggtag acgatctcgg tgactccttg cgcggcgaat ccggcgatct tctctgtgat
    10081 ctgctcggcc gtgccggtca tcgccctcga gctggcaaac gggagcaggt cggcaacatg
    10141 gggctcgtca tggggattgg cctgcacgag atgccccgca tggatggcaa gatgccgctc
    10201 gtcttcgggg caggcctcga tcgcctcgcg ccagcttcgt ccgcccggca ggtggtcgac
    10261 cgcggcggcg ccgccgtgct cgtacacggt gtggtaaatg accaccgcac cgggccccgc
    10321 ggcatcgacg gcacgcggcg acatcacttc ctcgccctcg tcgagcaccg tgccgagaca
    10381 cagcagcgcc tgccagtcgg ccaccttcgc ggcgtctggc tgcggtatgc cggcggagaa
    10441 cacgccgtcg cccagctcgg cggcaacggc caaccccttc gggcctccgg cgccgataag
    10501 tatcggcacc tcgaccggtc gctgcgcccc gaagccgggc aagtggagca tgcggatctt
    10561 cgcgccctcc cactccgcgg tttcgccggc cagcaaagcc ttcacgcagc gcacgtactc
    10621 ggcgaccttc cgccatggca tcggccgctg ccccagagcc atccgaccgg taagtcccga
    10681 gccgatcgcc accaccaccc gcccgggcgc ctggtccacc aactgggcga tcgccgcggc
    10741 gttgaccatg ggatgccgca ggctgggaac gagcacaccc ggtccgaggc cgatgcgcga
    10801 cgtgcgctcc gcacaccggg tcaggatcat ccacacgtcc gggtagagcg cgggagagtc
    10861 gtagagccac gcccgccggt agcccagtgc ttcggccaac tccacgtgag ccggagtgtc
    10921 cgacgacgtc gcgaacgcac aagagatgtc catgctcaaa cactaagcgc tggcggagcc
    10981 gccgagccgg atcagtccgt gcgcccgaga cgggcggcgt cttccaggag atcggcggcg
    11041 cgcccgacgc tttcggcggg tttggtcatc tcggcggcga cctcgcgggc ccgagcgacg
    11101 tactccgggg tgcggatgca gcccaggtcc gcgaccagtg attcctcggt ggtggccgcg
    11161 aacgggcgcc cggagccgac tcccagccgt tccagaccgg ccgcccacac cggctgatcc
    11221 agccagagcc agaggatcaa cgcggggatt ccggcccgca ggcctgcggc cgtggtaccc
    11281 gcgcccccgt ggtggacgac cgcccggcac gccggaagaa ccgtcgcgtg attcaccgcc
    11341 accacgacct tgacgtcgtc gaaacgcggg aggttagtaa ggtcattcgg gccgccgcaa
    11401 atcaacgccc gctcacccaa ccgcgcacac gccgcgccga tcatggcgat cgtgtgagcg
    11461 ggcgacgcga tcggcgtgct gccccacccg aagcagatcg gcggcgctcc cccggcaatc
    11521 caggacaaca cctcgtcgtc gccgtccgtc gccatctcca atgtgagcgc cccgacgaag
    11581 ggccgccgcc cgtccgttcc cacccattcg gccgcggggc cgggcaggca cagctcgtcg
    11641 taggcctgga tctccagcga ggccgccgcg gattccgtgg cctccggcaa gccgagggca
    11701 ctgcgctgtg cgtcggcgat gatattcgtc atgcccgagt ggatccctgc gggcaaaatg
    11761 cacgccggga aaaagtgcag cgcggccagc gggatgcggc aatactcggc gacggtggcg
    11821 gccagcgact gctcattgat gcccgccaac agcaggtcgg cctcttctgc cagtgacgtc
    11881 agagtggtgg tcttggcggt ccacacctcc gtgagatcct gcagcgctgc gggcaacgcg
    11941 ctgatcgggt tcgccgcggt gctaatgaaa tccgcggccg ttttcatctg cttccgcgag
    12001 tcgggtccgt aggcgatcgc ggtaaggccc gccgactcga cgaagcccgt catgtccggc
    12061 gataccgcca tgcacacgct gtgaccccgg cgcagcagct cgcgaccgac ggtcgcgcag
    12121 ggctcgacat cgccgcgact tccatagcct gccagcacga atttcatcgg cgcgaccgta
    12181 gcattccggg gcggtcacgg cgagatagcc tgggtgggct gcgcaacccg gcggcccgac
    12241 ggtttgacgg gcatgcttac acgtatgcat gtgatcaacg gtgttcgcga ccccgcggcg
    12301 agttttcccc tcgacaccgc ggcggacgat acggccgagc gtcgccaggc caaccgcgcc
    12361 ctcgccgtca gcgccgtcgg attggcattg accgggctcg tcgaactggc catcgcgctg
    12421 atctccgggt cggtcgcgct gctcggcgat gcactgcaca acttgtcgga cgtgtctacc
    12481 agcgccctgg tgttcatcgg attcagggca tcccgcaagg tcgctaccga gcgttatccc
    12541 tacggacagg aacgggcaga agacctggcc ggcgtgggtg tggccgtggt gatttggggc
    12601 agcgcagtgg tcgccggctt cgaaagcgtc acgaagctgc tccgccatgg cggaaccggc
    12661 cacctgggct ggggcatcgc ggcggcggcc gccggcatcg cgggcaatca gctggtggcc
    12721 cgctacaaac tggtcgtggg ccggcggatt cgctcggcga ccatggtggc agacgccaag
    12781 cattcctggc tggacgcgct gtcgtcggcc ggggcgatgc tcggcctgat cggcgtggca
    12841 ctcggctggg gttgggccga cgcggttgcc ggaatcgtcg tcaccggatt catctgccac
    12901 gtcggctggg aggtgaccgc cgacatcgcg caccgcctac tggatggagt cgaccccgaa
    12961 atcatcacga cggccgaagc ggtcgccacc acggtccccg gcgttctgca cgcccatgcc
    13021 cgggcccgct ggaccgggcg gaccctgcgc gtcgaagtgg aaggcttcct cgcccacgac
    13081 accccacttg cggaggccga tcgaatcggt cgcaacgtcg cttcgacgct ggcgccgcga
    13141 attcccgaga tgcacagctt cagctgggcc gcgcgagccg cttctcacga atcactgtgt
    13201 gctggaaacg attccacggt ctcgctcagc tgacgcgcgc tgcccaaatc ggctcgacag
    13261 ctatgcgggc ggcgcgctct tgatggccgc atcctgcttg cgaccgagat cgagcacgaa
    13321 atcgggttgc acggggtcgt tctgcgggct ctcgaactcg aggacggtga agaccttgcc
    13381 ttcggtgaac atcacgatcc ctttggcctt ggcaccgtcc ggcgacggcc ccaccgccat
    13441 ctggccaccg gtgcccacgt cggccggcgt cggcggggcc ttcacgctca gctcctgaat
    13501 gcccttggcg ctctggtcga gcgcctgtgc cgccgcaccc gcgtcgggat acacgtaaat
    13561 ggtgtcgtcg accttgcggg tgccgccctg attcatgaac acgccttcaa cccccgccgg
    13621 gttgggcacc ggcagcgtct gcatcagagt gaaggtgtcg ccgggcacca cgatgtcagc
    13681 gggcttgatc agcagaccgc tgtaatcgga aggctgggcc gcaccgctgc tcggtgtcgg
    13741 tgtcccggac gacgacgcgg cggacgacga cgccgcagtc gaagttgtcg acgttgccga
    13801 cgaggggcct gacgatttgt cgccgccaca cccggcaacc cccaccgcga ccactaaagt
    13861 gctcgtcacc caaccggcgg ccgcaaccga taccttcatc accattgact ccttcgattc
    13921 cggtcggcgg ttgccggcgt ctcgcgcaac ataccggacc cgctggcgcc gtggcggtcg
    13981 gcgtggacat cggcgcgcaa ctttagggaa tggcgaagtc agcgtcgacg gaatcgctcg
    14041 cgcagttccg ggtcgttctc ccaccacagc cccgatggcg cggcggtttc ggtccgggtc
    14101 actgccgccg agtccagctc ggcgtcgacc gcgacagcgt gcgccttctc gtcgcgggac
    14161 agggcgcgca tcagcaacaa cacgaacggc cacccgacca ggtcgccgag aatccacagg
    14221 acgccggcgc cgatggtctg atcgagccgc tggtcggggc cccacgaccg gtgcaattcg
    14281 gagtaatagc ccgcgccgat caacgagccc tgccagacca ccagccccag cacaccgtca
    14341 ccgagggcct cggcgacggt gatcagcaac gagatcagct gcgggtactt gcggggcacc
    14401 gggtccgcct gcagtcgggc gtagaaatag ctaaccccta tggctaccaa gaggattcgc
    14461 gtgggtgcgc cgatccattc gttttccagg gacgccgtgt accacggcgt caggtagagc
    14521 agccacggcg tgccgagcat cgccagagac gtcgtcagcg ggtgggcaag gacccgcgcg
    14581 aacggggtcg ccagccaccg gtcgatgcgc tcacggcccg cgggacccag cgcatcgcgc
    14641 accacggtga ccggcttgcc gaccgcgatg aagaacggca cgacgtaaag cagcatcaac
    14701 acctgcagcg cacgcatcca gaacaaggcg tacgcataaa cgccgatcac gctcgccgtc
    14761 gccagcaccc acaacacgat gcccaccccg aagcaggccg cctgcgcggg tcgcacgggt
    14821 tgctcggcgc ttcgggcgcg ccgatagcac cacgcatatc cgccggccac caacacgatc
    14881 accgcagcag aggccaggtc cagatgccag gtctggacta aggcggccca tgtcagcggc
    14941 cccggatcga cgcgcatggc cactagcgta ttgctgcccg cgaacgtggc aagcaggtcg
    15001 gcgctcctcg ccgatcgcgc gcccgcggtc ggcgaggacc ctaagttata cgggtggctg
    15061 ccatcttcat cgtgctcgac gtctttgtcg gcatcctcgc gctgctcggg gtcatcgcgc
    15121 tggggagtcg gctcggtgca cgacgccgca cgccgggcgt caaaggcgtc ctattggtga
    15181 gcgaacgcct ggcgccgcgc cgcaagaatg tcgcgcgatt ccagacgagg ctgcggatgg
    15241 tcgggccggt ggtgcgctac gaggtcgccc tcgacctgga agccgacggc cagcgattcg
    15301 aaacatcctc ggcccagcct gcaacacgtc cgtcgatggg ctgcgacgac gaggaattgt
    15361 cgtggagctt cgaggttcct gaagacgacg tcgaccggct ctgggtgatc gcctcctggg
    15421 tggaggggcg cgggcacgac ctgcgcagcg ccgcgctcac gcaacagttg agcacgtcgg
    15481 aaacctacga gtggaagtgg tatctgcggt ccctcggcgg ctggcaaaag cggcgaacgg
    15541 ccacgacgcc cacggcgacc gagcccgggc gggggccgct ggaactgggt cacgcacccg
    15601 accatcgacg tgaatcacca cccgctgccg agcccgacac caccgaagag gagcccggtc
    15661 acgagtgagt cgccgggcgg gtgcattccg ctggatccgc tatgaatcgc cgccgggctt
    15721 accgccgttg gtcgtcagcg ccgtcgggca gtagacgttg gcggcgatgg ccgtgaactt
    15781 ggccgcgttg tcgccgtgca gcccagagtt ctgggcttgc accgtcttga cgatgtcaac
    15841 catctgcgtg ccctgatcca ccgcttggca cacccatctg ccggccgcga tgacccggcc
    15901 ggggtcgggg tacgtgatgc ctgccgcctg cagtgacgcg aggaatgcgt catcgttgct
    15961 gtcggcgcgc
//
